LOCUS       DASPGN010000001         7276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_936768, whole genome shotgun sequence.
ACCESSION   DASPGN010000001 DASPGN010000000
VERSION     DASPGN010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936768"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VD788_00005"
     CDS             complement(<1..424)
                     /locus_tag="VD788_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74672.1"
                     /translation="MKRRAAVWAGGLALLGLCWARCSPAPPDPPDPRGLDVEPLVEQR
                     IVEATAAVRAQPESAEAWGRLGVIYDIHRLGDRALPCYDRAASLDPEDWRWPYFIGLT
                     HRESDQQAALRAFERAVELRPDYPPLRFYLGYGYLLSER"
     gene            complement(421..2133)
                     /locus_tag="VD788_00010"
     CDS             complement(421..2133)
                     /locus_tag="VD788_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HXV74673.1"
                     /translation="MQAPRSIERFRLWVSVAAASIVVAACGSGRPDASAAARFVDVTE
                     RVGLDFDHDRADRDHFMPDSLAAGCALLDYDADGDLDVYIVNGRYENGSVRHPAGANR
                     LYRQEADGSFTDVTEPSGAGDSGYGMGVAAGDIDNDGFVDLYLTNYGPDALYRNNGDG
                     TFTDVTAAAGVDNPRWATSAGFFDYDADGDLDLFVANYVAFEPSVRAVDLAGASEYPG
                     PECCPAEPDVLFRNTGDGTFEDVSRATGISHAIGRGLGVALIDLNEDGRLDIYVANDR
                     ENNRAWIQTADGRFVDRAVEMGIAVNLYGAAEASMGVAIGDADGDRRVDLFLTHLYQE
                     TNTLYLHTTPDRFEDATIGSGLGQVSIDFTGFGTAMIDYDLDSDLDLLVVNGRVLRSA
                     THPRASIDPHWRPYAEENLLFRNEGRARFEPAPRECGTLCSEIEVSRGLASGDVDDDG
                     AIDLLTTSGDGRVRLYRNTGPGTAHWLTLRVVEHNRDAYGATVLLDAGGTELRRDVLA
                     TDSYLSSRDPRVHFGLGGTMRIDRVRVRWVDGTVELFDVRELDRAYRLVRGEGRPASR
                     EGRR"
     gene            complement(2182..2697)
                     /locus_tag="VD788_00015"
     CDS             complement(2182..2697)
                     /locus_tag="VD788_00015"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HXV74674.1"
                     /translation="MNQKGMTLVELVVVVALVAAVALASAIYAVSWISRERARSAVYQ
                     LQSFMQLARIEAVSRNQDCRFLVDVSTRTLQVLDSNGTSTAADDILLHETRLPEVVTF
                     ARPDSGNAVTLSQIGSTSVYETVFSSDGVVSLGTGIVALYGGEEFNRVQVQGAGGVMV
                     QQWDGSTWGSV"
     gene            2901..3503
                     /gene="lexA"
                     /locus_tag="VD788_00020"
     CDS             2901..3503
                     /gene="lexA"
                     /locus_tag="VD788_00020"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXV74675.1"
                     /translation="MGRTPPGQTRQRILRFVRDRLLRGAPPTVREVQRQFGFRSVQTA
                     RQHLESLVEQGSLAKEQGKARGYRLPADETPTVLVPLLGRVPAGPSDLAVEELDDYLP
                     IRSRRAPGELFALRVRGESMKDAGILPDDVVVVRRQKTARSGEIVVALIGDEATVKRL
                     HIRGRRVELRPENPRFEPIVPRSGEMTLLGRVIEVRRSLE"
     gene            3500..4135
                     /locus_tag="VD788_00025"
     CDS             3500..4135
                     /locus_tag="VD788_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74676.1"
                     /translation="MNGLGVFLPPELDRHGVRRGVARSETPVRWQLSVFSGRFGEISG
                     DEAGVGLSLVFRLVLEAQRGGEPAVWIGRRDSVFFAPDVAETGVDLAALPVIRVADPL
                     SAARVADHLLRSGAFGLTVIDLGAHATLPLHAQVRLVGLAKKHHTALVCLTEKEGHRP
                     SLGSLVSLRVHAVRTQREGTRFHCEAQILKDKRRGPHWRHREIYRGPDGLC"
     gene            4122..5600
                     /locus_tag="VD788_00030"
     CDS             4122..5600
                     /locus_tag="VD788_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74677.1"
                     /translation="MACVDLPAFPLQLLLQRRPEWAAHPVAVVDADKPQGTILWVNER
                     ALAHRIRPGMRYAAGLSLAAALRAAVVPSAEIEHEIDGLVGRLRRFTPNVEPSAEEPG
                     VFWLDASGLERLHDSLRGWAGAVRAGLQQVGFRSTVVVGFSRFGTYALAKTKRDTVVI
                     GSEREEQSAAFRVPLDRLAIPPAARDALERLGVTTVGRLAELPVEGVERRFGPRVYRL
                     HRMATGERREPLQPVPPPPPSTVRTTLDHPETDVLRLLVVIERLLHPLLRASADRAAA
                     LVELQLGFRFESLGDHIETVRPAAPTLQAKQLLELIRLRLQAVRRLPDGVTEIVLISR
                     ETAATTRQLRLFAGKPKRDLEAGNRALARVRAELGDRAVVRAQLREAHMPEGRIVWEP
                     LDALQPAAPRQIDTGAMVRRIHTRPVPLPPRPRQEPDGWLLRGLQQGPVVRVSGPYVV
                     CGGWWNRPIHREYHFAETRKGELLWVYYDRVRRRWYLQGRVE"
     gene            5604..>7276
                     /locus_tag="VD788_00035"
     CDS             5604..>7276
                     /locus_tag="VD788_00035"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXV74678.1"
                     /translation="MSPSPVSYAPLWCKSNFSFLEGASHPEELVETCSAMGLEAMALT
                     DRDGVYGVVEAHVKARELGVKLILGAEITIEDGSSIVLLAIGREGYANVCRLITLGRR
                     RSPKGESRVGWREVCEHAGDVIGLWGGDRSLLVGEPDPFFVAHELREAFGDRLYALIA
                     RHRRDDEPAREGRLRQRAERYDLPTAAAVEVLYHKRSRRELQDVLTCLRYRVKLSEAG
                     RSIKANAEHALRAPHAFARLFHDDPASVARTRDVAGRCDFSLARLRYRYPSERLPDGK
                     TSSVWLRELTLAGARERYGRQLPAEVVEQLDKELQLIDELDYCGYFLTMWEIVRFCRE
                     RGILCQGRGSAANSAVCYCLRITAVDPVRLGLLFERFLSRERAEPPDIDLDIEHDRRE
                     EVIQHVYAKYGRSHAAMVANFIRYRPRSAVRDVGKVLGIAETSLDRLSKLLPHYSGVP
                     QEVMVQAGLDPEQPVHADLLRLSAELVDFPRHLSIHPGGFLLGHEPVSRIVPIENATM
                     PDRTVIQWDKDCLEELGLFKVDLLGLGALRQLHLGFDLLERHYGTVWSMA"
BASE COUNT         1123 a         2397 c         2529 g         1227 t
ORIGIN      
        1 gacgctcgga caacaggtag ccgtagccga ggtagaagcg gagcggggga tagtcgggcc
       61 gcagctcgac ggcgcgctcg aacgcacgca acgccgcttg ctggtcgctc tcgcgatggg
      121 tcaggccgat gaagtacggc cagcgccagt cctcggggtc gaggctcgcg gcgcggtcgt
      181 agcacgggag cgcccgatcg ccgagccggt ggatatcgta gatcacgccg aggcggcccc
      241 acgcctcggc cgactccggt tgggcgcgaa cggccgcggt cgcctcgacg atccgttgct
      301 cgacgagcgg ttcgacgtcg agcccccgag gatcgggtgg atcgggcgga gcgggcgagc
      361 acctggccca gcacaacccc aaaagcgcga gccctccggc ccagacggct gctcgccgtt
      421 tcatcgtcgt ccctcccgcg aggcggggcg tccctcgccg cggaccagtc gataggcacg
      481 atccaactcg cgcacgtcga acagctccac ggttccgtcg acccagcgta cacgcacgcg
      541 gtcgattcgc atcgtcccgc cgagaccgaa atggacccgc gggtcgcggc tcgacaggta
      601 gctgtcggtg gcgagcacgt cacgccgcag ctcggtgccg cccgcgtcga gcagcacggt
      661 cgcgccgtat gcgtcgcggt tgtgctcgac cacgcgcagc gtcagccaat gggccgtccc
      721 gggacccgta ttacggtaga gtcgcactcg cccgtcgccg ctcgtcgtca gcaggtcgat
      781 cgccccatcg tcgtcgacgt cgcccgaggc caggccacgg ctgacctcga tctccgagca
      841 cagcgtgccg cactcccgcg gtgccggctc gaaccgggcg cgaccctcgt tgcggaacag
      901 caggttctct tcggcgtacg gccgccagtg cgggtcgatg ctcgctcgcg gatgcgtcgc
      961 cgagcgcagc acccgaccgt tgacgaccaa gaggtcgaga tccgaatcga gatcataatc
     1021 gatcatcgcc gtaccgaaac cggtgaagtc gatgctgacc tggcccagtc cgctgccgat
     1081 cgtcgcgtcc tcgaagcgat ccggtgtcgt atgcaggtag agcgtgttgg tctcctggta
     1141 caggtgggtc aggaacaggt cgactcgccg gtcaccgtcg gcatcgccga tcgccacgcc
     1201 catgctcgcc tcggccgccc catagaggtt gaccgcgata cccatctcca ccgcgcgatc
     1261 gacgaagcga ccgtcggcgg tttggatcca ggcgcggttg ttctcgcggt cgttcgccac
     1321 gtagatgtcg agccggccgt cctcgttcaa gtcgatcagg gcgaccccca ggccgcgtcc
     1381 gatcgcgtga gagatcccgg tcgctcgact gacgtcctcg aacgtcccgt cgccggtgtt
     1441 gcggaagagc acgtcgggtt cggcaggaca gcactcgggt cccgggtact cggaagcccc
     1501 ggccaggtcg accgcgcgca ccgacggttc gaaggcgacg tagttggcga cgaacaggtc
     1561 gaggtcgccg tcggcgtcgt agtcgaagaa accggcggag gtcgcccagc gcggattgtc
     1621 gaccccggcc gccgcggtga cgtcggtgaa cgtgccgtcc ccgttgttgc gatacaacgc
     1681 gtcgggcccg tagttcgtca gatagaggtc gacgaacccg tcgttgtcga tgtcgccggc
     1741 ggcgaccccc atgccgtacc cgctgtcgcc cgcaccggac ggctcggtga cgtcggtgaa
     1801 cgacccgtcc gcttcctgac gatagagccg gttggccccc gcgggatggc gcacgctgcc
     1861 gttctcgtag cggccgttga cgatgtagac gtcgagatcg ccgtccgcgt cgtaatcgag
     1921 caacgcgcaa ccggccgcca gactgtccgg catgaagtga tcccggtcgg cgcggtcgtg
     1981 gtcgaagtcg agaccgaccc gctcggtgac gtcgacgaag cgcgccgccg cgctcgcgtc
     2041 gggccggccg ctcccgcacg cggcgacgac gatcgacgcc gcagcgaccg acacccagag
     2101 ccgaaaccgc tcgatcgacc gcggggcctg catccctggg aatcgtagca ccggacccag
     2161 gaggacggat cggatgggcc gttagacact accccaggtg ctgccgtccc actgctgcac
     2221 catcacgcca cccgcaccct ggacctggac gcggttgaat tcctcgccgc cgtacagcgc
     2281 gacgatgccg gtgccgaggg agaccacgcc gtccgagctg aagaccgtct cgtagacgct
     2341 cgtcgagccg atctgggaca gcgtcacggc gttgccgctg tccggtcggg cgaaggtgac
     2401 gacctccggc agtcgggtct cgtgcagcag gatgtcgtcg gcggcggtgg acgtcccgtt
     2461 cgagtcgagg acctgcaggg tgcgcgtgga gacatcgacg aggaaacggc agtcctggtt
     2521 gcgactgacg gcctcgatac gcgcgagctg catgaagctc tgcaactggt agacggccga
     2581 gcgagcccgc tcgcgggaga tccacgagac ggcgtagatc gcgctcgcca gcgcgaccgc
     2641 ggcgaccagc gccaccacga ccacgagctc gaccagcgtc atacctttct ggttcatcgg
     2701 tcggtcctcg gccccatcct tgaactgaat ctattcgcgg cccccccgga ttcaattaca
     2761 ggaatcgtaa gtcgtgacct ggcggtggtc ggatcgcccg gggaatttgt aactattcgg
     2821 gagtcggtgg atcgggattg ccataccata catttgtgtg gtatgatcga gctaccggaa
     2881 caacaacgga gtgtgccgtc atgggacgta ccccgcccgg acagacgcgt cagcggatcc
     2941 tgcggttcgt tcgcgacaga ctgttgcgtg gagccccgcc cacggtccgc gaggtgcagc
     3001 ggcagttcgg tttccgttcc gtgcagaccg cgcgccagca tctcgagtcg ctggtcgagc
     3061 agggcagcct ggccaaagag cagggcaagg ctcgcggcta tcgtctgccg gccgatgaaa
     3121 cgccgacggt gctcgtgccg ctactcggtc gcgtgccggc cggtccatcg gacctcgccg
     3181 tcgaagagct cgacgactat ctgcccatcc ggtcgcgacg cgcgccggga gagttgttcg
     3241 ctctccgcgt ccgcggcgaa agcatgaaag acgcgggcat cctgcccgac gacgtggtgg
     3301 tggtgcggcg tcagaagacc gctcggtcgg gagagatcgt cgtcgccttg atcggtgacg
     3361 aagccacggt caagcgcctg cacatccgtg gtcggcgagt cgaattgcgt ccggagaatc
     3421 cgcgtttcga gccgatcgtc ccgcggtcgg gcgagatgac gctgctgggc agggtgatcg
     3481 aggtccggag gtcgctcgaa tgaacgggct cggagtgttc ttgccgccgg agctcgatcg
     3541 ccacggtgtg cggcgtggcg tggctcgcag cgagactccg gttcgctggc agctttccgt
     3601 cttcagcgga cgcttcggcg aaatctcggg cgacgaggcg ggcgtcggct tgagcctggt
     3661 cttcaggttg gtgctcgagg cgcagcgcgg gggcgagccc gccgtctgga tcggccggcg
     3721 cgacagcgtg ttcttcgcac ccgacgtggc agagaccggt gtcgacctgg ccgcgctacc
     3781 cgtcattcgg gtcgccgatc cgttgtcggc cgcccgggtc gccgatcatc tcttgcgctc
     3841 cggtgccttc ggcctgacgg tcatcgacct gggggcgcac gccaccctgc cgctccacgc
     3901 gcaggtccgt ctcgtcgggc tggccaagaa acaccacacg gccctggtgt gtcttaccga
     3961 gaaggaaggg caccgcccgt cgttgggctc actcgtctcg ttgcgtgtcc atgcggtacg
     4021 cacgcaacgg gaaggaactc gtttccactg cgaagcgcag attctcaagg acaagcgccg
     4081 cgggccgcac tggcggcacc gggagatcta tcgtgggccg gatggcctgt gttgacctgc
     4141 cggccttccc gttgcaactg ttgttgcaac gccggccgga gtgggccgcc cacccggtgg
     4201 cggtcgtcga tgccgacaag ccccagggaa cgatcctgtg ggtcaacgaa cgggcgctgg
     4261 cgcatcggat ccgtcccggg atgcgctacg ccgcgggtct gtcgctggcc gccgcgttga
     4321 gagcggccgt cgtgccgtcc gccgagatcg agcacgagat cgacggcttg gtcgggcggt
     4381 tgcggcgatt cacgccgaac gtcgagccgt cggcggaaga gccgggcgta ttctggctcg
     4441 acgcgagcgg gctcgagcgg ttgcacgact cgttgcgtgg atgggccggg gcggtccgtg
     4501 ccgggttgca acaggtgggg ttccgctcca ccgtggtggt cggtttcagt cggttcggaa
     4561 cgtacgcctt ggccaaaacc aagcgtgaca cggtggtcat cggctccgag cgcgaagagc
     4621 agtcggcggc gttccgcgtc ccgctcgatc ggttggcgat tcctcccgcg gcacgagacg
     4681 cgctcgagag gttgggggtg accaccgtcg gacgtttggc cgagctgccg gtggaggggg
     4741 tcgaacgacg cttcggccct cgggtctacc ggttgcatcg tatggccaca ggcgagcggc
     4801 gtgaacccct gcagccggtg cccccgcctc ctccgtccac ggtgcgcacg acgctcgatc
     4861 atcccgagac cgacgttttg cggctgctgg tcgtgatcga gcgtctgctg cacccgctgt
     4921 tgcgagcgtc ggccgatcgg gccgcggcgc tggtcgaatt gcagctcggc tttcgtttcg
     4981 agagcctggg cgatcacatc gagaccgtcc ggcccgcggc acccaccctg caggcgaagc
     5041 aattgctcga gctgatccgt ttgcgattgc aggcggtgcg acgtttgccc gacggggtca
     5101 cggagatcgt gttgatctca cgcgagaccg cggcaaccac gcgtcagcta cgactgttcg
     5161 ccggcaagcc gaagcgcgac ctcgaagccg gcaaccgcgc gttggcccgc gtgcgtgcgg
     5221 agttgggtga tcgggccgta gtgcgcgcgc agctacgcga agcgcacatg ccggaagggc
     5281 gtatcgtctg ggagccgctc gacgcgttgc aacccgccgc tccgcgccag atcgacaccg
     5341 gcgcgatggt gcgcaggatc cacacccgac cggtgcccct gcctccgcgg ccgcgtcagg
     5401 agccggatgg ttggctgttg cgcgggctgc agcaaggtcc ggtggtgcgg gtgtccggtc
     5461 cctacgtcgt ctgcggcggt tggtggaacc gcccgatcca tcgcgaatac cacttcgccg
     5521 agacacggaa gggcgagttg ctgtgggttt attacgatcg tgtccgacgt cgctggtacc
     5581 tccagggtcg ggtcgaatga ccgatgagcc cctctccggt gagctacgcg ccgctgtggt
     5641 gtaagagcaa cttctcgttt ctcgaggggg cgagccaccc cgaggagctg gtcgagacct
     5701 gctcggcgat gggtctcgag gcgatggcgt tgaccgatcg ggatggggtc tacggcgtcg
     5761 tcgaagcgca cgtcaaggct cgcgagctgg gcgtcaagct gatcctcggc gccgagatca
     5821 cgatcgagga cgggtcgtcg atcgtgctgt tggcgatcgg tcgcgagggg tacgccaacg
     5881 tctgccgcct gatcacactc ggacgccgtc gctcgcccaa gggtgaaagc cgcgtcggct
     5941 ggcgtgaggt gtgcgagcac gccggcgacg tgatcggcct gtggggcgga gaccgcagtc
     6001 tgctcgtcgg cgagcccgac ccgttcttcg tggcgcacga gctgagagag gccttcggcg
     6061 accgactgta cgccctcatc gctcgccacc gtcgtgacga cgagccggcg cgcgagggcc
     6121 ggctgcgcca gcgcgccgaa cgctacgatt tgccgacggc ggcggccgtc gaggtgctgt
     6181 accacaaacg atcccgccgc gagttgcagg acgtgttgac ctgcctgcga tatcgagtca
     6241 agctgagcga ggccggccgc tcgatcaagg ccaacgccga gcacgcgctc cgagctccgc
     6301 acgccttcgc ccgcctgttc cacgacgacc cggcgtcggt agcgcgcacg cgcgatgtcg
     6361 ccggtcgttg cgatttctcg ctggctcgac tgcggtaccg ctatccgtcc gagcgacttc
     6421 ccgacggcaa gacctcgtcg gtctggctgc gagagctgac tcttgccggg gcccgcgagc
     6481 gttacgggcg acagctaccg gcggaggtgg tcgagcagct cgacaaggag ctgcagctga
     6541 tcgacgagct cgactactgc ggctacttcc tgacgatgtg ggagatcgtt cgtttctgcc
     6601 gcgagcgggg gatcctgtgc caagggcgag ggtcggcggc caattcggcc gtctgttact
     6661 gcctgcggat cacggccgtc gatccggtgc gcctgggcct gctgttcgag cggttcctct
     6721 cccgcgagcg cgccgagccg ccggatatcg atctggacat cgagcacgac cgccgcgagg
     6781 aggtgatcca acacgtctac gccaagtacg gtcgaagcca tgccgcgatg gtggccaact
     6841 tcatccgtta tcggccgcgc tcggcggtac gcgacgtagg aaaggtgctg gggatcgccg
     6901 agacctcgct cgaccggttg tccaagctgc tgccgcacta cagcggggtg ccgcaggagg
     6961 tgatggtcca agccggtctc gatccggagc aacccgtgca cgccgatctg ctgcggctgt
     7021 ccgccgagct ggtcgacttc ccccgccacc tgtcgattca tccgggtggt ttcctgttgg
     7081 gtcacgaacc ggtctcgcgg atcgtcccga tcgagaacgc gacgatgccc gaccgcacgg
     7141 tgatccagtg ggacaaggac tgcctggaag agctggggtt gttcaaggtc gatctgctcg
     7201 gtctcggggc gctgcggcaa ctgcacctgg gtttcgatct gctcgaacgg cactacggaa
     7261 cggtgtggag catggc
//
LOCUS       DASPGN010000002        13993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_468594, whole genome shotgun sequence.
ACCESSION   DASPGN010000002 DASPGN010000000
VERSION     DASPGN010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 13993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13993
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468594"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1946
                     /locus_tag="VD788_00040"
     CDS             <1..1946
                     /locus_tag="VD788_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV74679.1"
                     /translation="TQGGFPKLVLEAKREFASIYGLQADYWRHFTPEESPEVLAYLKA
                     NLKDLATHYHAEYQGIAEGDEDGKLASFSEARRWYGDYLASFPTDAETPSINYRLADL
                     LLEHDQFGDAARQYERTAYAYEPHEQSAAAGYAAVYAYREQLGVASDEQKEAVQQDTV
                     LSSLRFADAFPEHEHAATVLGAAADDMYEMKDYRAAIESAQRVIDSYPAADGAIRRSA
                     WIVVAHGSFELAEYPQAERAYAQVLAVTPEDDESHAAFVDNLAASIYKQGEAANAAAD
                     HRAAADHFLRIRSAAPTSTIRATAEYDAGVALIALESWTEAAGVLEAFRSTHPEHEMQ
                     REATRLIAYAYRENGQLSRAAGEFDRLASESDDPAVRSEALLVAGDLYQQSDAPDRAL
                     QVYTRYVDEFPQPVEMALETRFKIAEIHKAAHDEWRYHAELAAIVQLDAEAGAHRTAR
                     TRTLAGRSALVLAERLYHEFVAVKLSQPFEVSLQEKKRRMDAVIETMGGLVAYEIGEV
                     TAAATYYMAETYFDFSRSLVDSERPVDLEPADLEQYELALEEEAFPFEEKAIDVHEKN
                     IELLQAGLFNGWTEKSLGKLAELVPGRYAKNEMSSDFAGSIDSYVYRSPLCQLAGTTD
                     SVEATQLAPTAIDELVVDDANPQ"
     gene            1927..2628
                     /locus_tag="VD788_00045"
     CDS             1927..2628
                     /locus_tag="VD788_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV74680.1"
                     /translation="MMRTRSDTGSAKALCRSVPWLALVLAVGCSTGDRRPVTAYESTP
                     SDEPQDELAALIDVVQAADGFTITQQVEVPDDVRADYDAAIRMLEVARYEPAIALLLD
                     VTESEPGVTAAHIALGIAYAQVGKLDDAEAGLRRALELNPLHPVAHNELGLVQRRKGQ
                     FAESRASYEAALAQFADFHYAHRNLAILCDLYLGDYPCALEHYEAYGRIVPDDAEVVK
                     WIADLRNRTTPQENP"
     gene            2625..3002
                     /locus_tag="VD788_00050"
     CDS             2625..3002
                     /locus_tag="VD788_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74681.1"
                     /translation="MSRILLFATLLLATSVVPADDVVEAESAAGKSETQVQDEAPETD
                     GATSASEAQPQKEDGPKLVSGMSILGNQEAPKSLVIVPWKSSEIGDSIGISTMLDDSR
                     QPIDKEVFMRALSYYEIRSETTP"
     gene            3124..3723
                     /locus_tag="VD788_00055"
     CDS             3124..3723
                     /locus_tag="VD788_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV74682.1"
                     /translation="MYPILIVFAIGIAIAIERYITLTIVRRRNEGMWTRLQPALASGD
                     FDKARQMTDKDDSTVSRLLAMGLARQGAVRRREEIEIAMEESMMEIIPQLEKRTAYVA
                     LGASIATLLGLLGTIIGLIQAFTAVANANPAEKADLLSASISVAMNTTAFGLIAAIPL
                     LVTHSVLIAKTGEIVDSLEMASVKALNVITSMAKRQAAV"
     gene            3729..4211
                     /locus_tag="VD788_00060"
     CDS             3729..4211
                     /locus_tag="VD788_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV74683.1"
                     /translation="MPRAHHYRRRHKEAPELDMTTFMNLMVVLVPFLLITAVFSRITI
                     VELDLPSAQSAAPTEPTFRVEVVVREAGLEIMDGAQVIAAIPKTGDGYDLSTLSDYLV
                     AIKREYPQKDDASVLLEPDIPYDHLVQVMDTVRSVELLDGGQTMRADLFTAISIGDAP
                     "
     gene            4235..4705
                     /locus_tag="VD788_00065"
     CDS             4235..4705
                     /locus_tag="VD788_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV74684.1"
                     /translation="MARRKRAASKLNLTPLMDVFTVLVFFLMVNSAAVETLQQPKQIK
                     LPESVVEAKPRETVVIFVGTDQVLVQGVPVARIADIQATDNVEIEPISVRLAELSESV
                     IGLSTQAVAESQEVTVLADKSIPFSVLKKIMATCTAQGYTRVSLAVVQKSTQST"
     gene            4730..6202
                     /locus_tag="VD788_00070"
     CDS             4730..6202
                     /locus_tag="VD788_00070"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HXV74685.1"
                     /translation="MKSQQEQALRAKVEQVRGRLQGLTADLRVVDDEVERLAPQRTRH
                     ELLDQACSSLEKLGELGASSLFWGEQTAPSRIVEQLRGVRSRVSAFQAQLGELDERRQ
                     AILVQVGREEEVLEILGADIYYVREEEERRKLEWVVEREIGPVPPRLQRMAWARGGED
                     DRRFRRSLAASLLVGLLLGLVFPMIDLPLPKRFVPEEMPERLAQLVRQELVKPIPPPP
                     LVAETPPDQTPPEQQESKPIEQPEPAEQPPQVPAIADAEPSELPGQLGAETPSVAAAA
                     PGKTVEKAGILAFKDEFASLARDNVAPRLGADARYGDAADASRASSAARSMLTTNAPG
                     SSRGIDVSSLSRSVGGGGGGDGTGDGGGGGMQGVQVGRASSSIASIGGGGRPLARGGP
                     GPSRTDEEIQIVFDRYKASFYRLYNRELRNDPTLRGQMVLRLTIEPDGSVSMCKLQST
                     DMDAPALAAQVVERVLTINFGAKDVEALTIVYPIDFLPAA"
     gene            6231..11381
                     /locus_tag="VD788_00075"
     CDS             6231..11381
                     /locus_tag="VD788_00075"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74686.1"
                     /translation="MALRNSIGAKAGGGRTSFLLLLFALLLGASSDAQAIDCVADAGG
                     VIDGFVNYPVPPPQINIDGPCTIRNYTASNPLTSNISWFGNNPTSWLLIFDNVVHTGN
                     MSCNLAAQGNRIWFVNSASTTVQSNCLSLLIPVEKIDKANPPGPPFVTVGVPFSWTLT
                     IPVLFDPGTGTVINNQGSVNDLHSITVWDDLNATGVDLTYVSHTLTWLDDGTQVPHTF
                     TNVGGFLTFDNIPVTTAGRQFVIELTVVLEDTPVNAPGTQFVNTATWDFGRLIEGVFY
                     EPLPGENGISPPLTIAAPVLVMDKTGPATMNLGQSGDFVLDVQNTGSIDAWDVALRDL
                     FPDGPTGGMCDLQPQIVSAQVFAADGITPVPGKGPLAAGVDYTTTWGGAPSCRLDLTM
                     LTAAARIGPSERLIVRYRTQLDADTLDGAALTNVAGAIEWFNGASGNPERVATNRTLT
                     NGTVGVQDHEDAHTVTVALTGYFFEKTAANLTSGVDPTSTAAPGDTLRYTVRFRATDQ
                     ALGDFSIHDELDALNAPPAFVPGTLTLVSYPPGADISGTSSTGGADGTGVIDVGNLSV
                     PLDGEVVIEFDITLASTLDNGTVVTNQSAVRLTSGATFTLSDDPNVNGTADPLVPGDE
                     DPTRVTIVSAPDFRIHKISTDLTGDPNVLLAGETLRYTITVQNVGNEDALDVVLRDAV
                     PVNTTYVAGSTTLNGAAVADVGGLSPLVNGMLIHSPADPTPGSMPADASGNPANVATI
                     TFDVVVDTDVVDGTIISNQGFVSAVDVGIVDQPSDDPDTPIADDPTLDVVGNLPLLYA
                     EKRVALFGDLGSPGIVDPGDVLRYTITVHNSAAIPATGIVLTDSVPANTTYVADSTLL
                     NGLAVGQPDGGVAPLASGIDISSSDLTPPLPGPGQGTISPGATAVLQFDLRVDNGTPA
                     GTLISNQAVVDSVELPDLLTDGDGDPATGPEPTVVVVGDGQLLSITKQVAVVGGGPAV
                     PGAELEYVVRVVNIAAVPALGVVLTDDLDAVQPGQLTYVDLSATMNGSATGVSFAGST
                     ITADYDAVNGPLAPGEAVVLRFRAILDPALLNGTVVTNTGVVSWNTPAQTASASVSIV
                     VGGIPGFAALNGSAWHDADFDDARDSTERALAGWSVELYGDGQLLHSALTDANGVYRI
                     VGVEPNDVSGTVYELRFSAPGSGASTAMLGQAASPFTNGMQRIGDILVPPDANLQGLD
                     LPIDPNGVVYNSVARIPVAGATLTLLDAGGGSPLPANCFDDAAQQGQVTLGDGYYKFD
                     VNFSDPACPPGAGYVIGVTPPPGTTYVAGYSQIIPPASDPSTAAFSVPACPGTVDDTV
                     PSTAQFCEVQPSEFAPASSVAARAAGTVHHVHVTLDGSQAPGSSQIFNNHLPLDPEVN
                     GSIAISKTTPLLYVTRGQQVPYTLRLNNLAGTLLTDVSIVDRFPAGFAYVEGSAVLDG
                     VPTEPTVAGRELSWSGLVVAGTEERTVKLLLAVGAGVSEGDYVNRAQVLNGVTGSAMS
                     GEATATVHVVPDPTFDCTDVTGKVFNDANRNGMQDDGEDGLPGVRVATARGLLATTDQ
                     HGRYHITCATTPNEIRGSNFVLKLDDRTLPSGFRMSTEPVRIKRATRGKVLRINFGAS
                     IHRVVGIDLSDAAFEPGTTEIRVQWRPRLDLLLDELRKAPSVLRLSYVADTEDAALVK
                     RRIDAVERQVTEAWDAASHGYRLTIEPEIFWRLGAPSRQPDGRLSDGR"
     gene            11387..>13993
                     /locus_tag="VD788_00080"
     CDS             11387..>13993
                     /locus_tag="VD788_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXV74687.1"
                     /translation="MGTHRHLLLIAGLLIAVPATADPVETTEPGESVERHLSDDQTFR
                     QWVQDPDLLDSERGDRLEVRQVVGEDLETVKLKNVVPPIRFKSGVAEIPPDYVDRLAK
                     VLASMRDRRNVRLHLVGHADSQPLSDALVRVFGDNAGLSRERAGEVAEYFKTALALPP
                     EAITYEWAGDAQPIASNLTDEGRALNRRVEVEVWYDEVREALRDEEVVVSDEIKRIKV
                     CRMETVCKLRYREGHARRARVRNLVVPLRYEDETTPISEDFLHQIRQALVNLRDKRNV
                     AVRFIGHTDDAPLTGRNERIYGDQLSLSKARALRVALAIQEALTLPTSAIESDGRGAS
                     RPLASNETAQGRKLNRRVEVEFWYDDPLQELPDEPQVCPGDVGEEMVTKVYDPPWGRI
                     PTLELVDGRPIVPPGYAADLHRALSDIADRTNARLRFIGYTKNERLDRRTASVYGDDI
                     GLSAARARRTMETVKQDPLLSSAPSEHEGRGYVHSDDVVNAGFIQGERSFVRVQVVYD
                     EPMPLDDYEGVDITRITRELRPKSAYELNVMRITVDGKPIDDPNRSSSDVQRCTDVAL
                     DDARIRFHFDNLASERRLAVAADPVAVAVSDPGDGPVASVVRFRMYSNYSSFIERAEI
                     RIFEQQQSLDAVPLEVIAIDDSGLAEWQPVVEPPAAPTRELAYVLRVYDSKGNFDETD
                     VRPLWLYREPAPDEVVPAAAETGPPDELLAAYGESDVERSRIRLGGSGTVRVQGSGIP
                     AGHTVWVAGRRVPVDPHGSFVAEEILPSGAHTVEVAVLDDAGSGSLYLRDLEFKRTDL
                     FYLGLADLTLSDTSANGPVEMLEGQDAPQPFDSSIDGRLAFFANGRVREHWQLTASAD
                     TREGPVEDLFSNF"
BASE COUNT         2402 a         4794 c         4737 g         2060 t
ORIGIN      
        1 tcacccaggg cgggttcccc aagctggtgc tcgaggcgaa gcgtgagttt gcctcgatct
       61 acggactgca ggccgactac tggcggcact tcacccccga agaatcgccg gaggtgctgg
      121 cctacctgaa ggcgaacctg aaggatctcg cgacgcacta ccacgccgag tatcagggca
      181 tcgcggaagg ggacgaggac gggaagctgg cgagcttcag cgaggcgcgc cgatggtacg
      241 gcgactacct cgcgtcgttc ccgacggacg ccgagacacc ctcgatcaac taccggctcg
      301 cggacctgct gctcgagcac gaccagttcg gcgacgcggc cagacaatac gagcgcacgg
      361 cctacgccta cgagccgcac gagcagtcgg cggccgcggg ctacgccgcg gtctacgcct
      421 accgcgagca gctcggggtt gcgagcgacg agcagaagga ggcggtccag caagacaccg
      481 tcctgagctc gctccgtttc gccgacgcct tccccgagca cgagcacgcc gcgacggttc
      541 tgggagcggc ggccgacgac atgtacgaga tgaaggacta ccgcgccgcg atcgagtccg
      601 cccagcgcgt gatcgacagc tatccggcgg cggacggcgc catccggcgc tccgcgtgga
      661 tcgtcgtcgc gcacggctcg ttcgagctcg ccgagtaccc gcaggccgaa cgggcctacg
      721 cccaggtgct ggcggtcacg ccggaggatg acgagtcgca cgccgccttc gtcgacaatc
      781 tcgccgcctc gatctacaag cagggcgagg ccgccaacgc cgcggcggat catcgcgcgg
      841 cggccgacca cttcctgagg attcgttcgg cggcgccgac gtcgaccatc cgggcgacgg
      901 ccgagtacga cgcgggcgtc gcgttgatcg cgctcgagag ctggaccgag gcggcgggcg
      961 tactcgaagc attccgcagc acccatcccg agcacgagat gcagcgcgag gccacccgac
     1021 tgatcgccta cgcctaccgg gagaacggcc agttgtcgcg cgccgccggc gaattcgacc
     1081 gcctcgcctc cgagtccgac gatccggcgg tccgcagcga ggcgctgctc gtcgccggag
     1141 acctgtacca gcagtccgat gccccggatc gcgcgcttca ggtctacacg cgttacgtcg
     1201 atgagttccc ccagccggtc gagatggcgc tcgagacccg gttcaagatc gccgagatac
     1261 acaaggcggc acacgacgag tggcggtatc acgcggagct ggccgcgatc gtccagctcg
     1321 acgcggaagc cggggcgcac cggacggctc gaacccggac gctcgccgga cggtcggcgc
     1381 tggttctcgc ggagcggctc taccacgagt tcgtcgcggt caagctctcg cagccgttcg
     1441 aggtcagcct gcaggagaag aagcggcgca tggatgccgt gatcgagacg atgggcgggc
     1501 tggtggccta cgagatcggc gaggtgaccg cggccgcgac ctactacatg gccgagacct
     1561 acttcgattt cagtcgctcg ctggtcgact cggagcggcc ggtcgacctg gaaccggcgg
     1621 atctcgaaca gtacgagctg gccctcgagg aagaggcgtt tccgttcgag gaaaaggcga
     1681 tcgacgtgca cgagaagaac atcgagctgt tgcaggccgg tctgttcaac ggctggacgg
     1741 agaagagcct cggcaagctc gccgagctgg tcccggggcg ctacgcgaag aacgagatga
     1801 gcagcgactt cgccggctcg atcgacagtt atgtctaccg atcgccgctg tgtcagctag
     1861 cgggcacgac cgactcggtc gaagcgaccc agctggcacc tacggcgatc gacgagttgg
     1921 tggtggatga tgcgaacccg cagtgacacc ggctcggcga aggcgctgtg ccgcagcgtg
     1981 ccctggctgg ccctggtgct ggccgtgggc tgctcgaccg gcgatcggcg cccggtgacc
     2041 gcctacgagt cgacgccgtc ggacgagccg caggacgagc tggccgcttt gatcgacgtc
     2101 gtgcaggccg ccgacgggtt caccatcacg cagcaggtgg aggttccgga cgacgtgcgc
     2161 gccgactatg acgcggcgat ccgcatgctc gaggtggcac ggtacgagcc ggcgatcgcc
     2221 ttgctgctcg acgtgacgga gagcgagccc ggggtgacgg cggcgcacat cgcactcggc
     2281 atcgcatacg cgcaggtcgg caagctggac gacgccgagg ccggcctgcg acgggcgctc
     2341 gaactgaatc cgctgcaccc cgtcgcccac aacgagctcg gtctggtcca gcggcgcaag
     2401 ggacagttcg ccgagtcgcg ggccagttat gaggcggcgc tggcgcagtt cgccgacttt
     2461 cattatgcgc acaggaacct cgccatcctg tgtgacctgt atctcgggga ttacccctgc
     2521 gcgctcgagc actacgaggc gtacggccgc atcgttccgg acgacgccga ggtcgtcaaa
     2581 tggattgccg acctgcgcaa ccgcacaacc ccacaggaga acccatgagc cggatcttgt
     2641 tgttcgcgac gctgctgctg gcgaccagtg tcgtgccggc agacgacgtg gtcgaggccg
     2701 agtccgccgc cggcaagagc gagacgcagg tgcaggacga agcgcccgag acggacggcg
     2761 ccaccagtgc cagcgaggcg cagccgcaga aggaagacgg tccgaagctg gtttccggca
     2821 tgtcgatcct gggcaatcag gaggcgccga agtcgctcgt catcgttccg tggaagagct
     2881 cggagatcgg cgactcgatc ggcatctcga cgatgctcga cgactccagg caaccgatcg
     2941 acaaggaagt cttcatgcgg gcgctgagct actacgagat caggtcggaa acgacgccgt
     3001 aggcgtcggt caagtttcgt cgttggtcga taaccgaagg gactcgagag gctagttgca
     3061 ggaggttcga cgatggatgc gatctattca gtggtccggt tcttcgtggc ggggggtcca
     3121 ttcatgtacc ccatcttgat cgtgttcgcg atcggcatcg cgatcgcgat cgagcgctac
     3181 atcacgttga cgatcgtccg gcgccggaac gaaggcatgt ggacccgact ccagcccgcg
     3241 ctcgccagcg gggacttcga caaggcgcgc cagatgaccg acaaggacga ctcgacggtc
     3301 tcgcgactgt tggcgatggg cctcgcgcgc cagggcgcgg tccgacgccg ggaggagatc
     3361 gagatcgcga tggaggagag catgatggag atcatcccgc agctcgagaa gcgcacggcc
     3421 tacgtcgcac tcggcgcgag catcgccacc ctgctcggcc tgctgggcac gatcatcggt
     3481 ctgatccagg cgttcaccgc ggtggcgaat gccaatccgg cggagaaggc cgacctgctg
     3541 tccgccagca tctcggtcgc gatgaacacg accgcgttcg ggctgatcgc cgcgatcccg
     3601 ttgctcgtca cgcattcggt gctgattgcc aagacgggcg agatcgtcga cagcctcgag
     3661 atggcgtcgg tcaaggcgct caacgtcatc acctcgatgg cgaaacgcca agccgcggtg
     3721 taacgacgat gcccagagcc caccactacc gccgtcgcca caaggaggcg ccggagctcg
     3781 acatgaccac cttcatgaac ctgatggtcg tgctggtgcc gttcctgctc atcaccgccg
     3841 tcttctcgcg gatcacgatc gtcgagctcg acctgcccag cgcgcagagc gccgcgccga
     3901 ccgaaccgac cttccgggtc gaggtggtcg tccgcgaggc agggctcgag atcatggatg
     3961 gcgcgcaggt gatcgccgcg atcccgaaaa cgggcgacgg ctacgacctg tcgacgctgt
     4021 ccgactacct cgtcgcgatc aagcgcgagt atccgcagaa ggacgacgcg tcggtgttgc
     4081 tcgaacccga catcccgtac gaccacctgg tgcaggtgat ggacaccgtg cggagcgtcg
     4141 agctgctcga cggcggacag acgatgcggg ccgacctgtt caccgccata tcgatcgggg
     4201 atgcgccatg aagacatcga ggcggatcgc acgcatggcg cgcagaaagc gcgccgcatc
     4261 gaagctgaat ctgacgccgc tgatggacgt cttcaccgtc ctcgtcttct tcctgatggt
     4321 caactcggcc gccgtcgaga cgctgcagca gcccaagcag atcaagctgc ccgagtcggt
     4381 cgtcgaggcg aagccgcgcg agaccgtcgt gatcttcgtc ggcacggacc aggtgctggt
     4441 gcagggcgtt ccggttgcgc gcatcgccga catccaggcg accgacaacg tcgagatcga
     4501 gccgatcagc gtacggctcg ccgaactgag cgaaagcgtc atcggcttga gcacgcaggc
     4561 ggtcgcggag agtcaagagg tgacggtgct ggcggacaag tcgatcccgt tcagcgtgtt
     4621 gaagaagatc atggccacct gcacggcgca aggttatacg cgagtgtcgc tcgcggtggt
     4681 ccagaaatcc acacagtcga cctaagacag atctgaccag gtgcacggca tgaagtcgca
     4741 gcaagaacag gcgctacgcg cgaaagtcga gcaggtccgc gggaggctcc agggtctgac
     4801 cgccgacctg cgcgtcgtcg acgacgaagt cgagagactc gccccgcagc ggacgcggca
     4861 cgagctgttg gatcaggcgt gcagctcgct cgagaagctg ggcgagctcg gtgcgtcctc
     4921 gctcttctgg ggcgagcaga ccgcgccgtc ccgcatcgtg gagcagctgc gcggcgtgcg
     4981 cagccgagtc agcgccttcc aggcgcagct cggcgagctc gacgagcgac gccaggcgat
     5041 cctcgtccag gtcggccgcg aggaggaggt tctcgagatt ctcggcgcgg acatctatta
     5101 cgttcgcgaa gaggaagagc gccgcaagct cgaatgggtc gtcgaacggg agatcggccc
     5161 cgtgccgccc cggctacagc ggatggcttg ggcccgcggc ggcgaggacg accggcgctt
     5221 ccgcaggtcg ctggccgcat cgctcttggt cggcctgctg ctcggcctgg tctttccgat
     5281 gatcgacttg ccgctgccca agcgcttcgt gccggaggag atgcccgagc gtctggcgca
     5341 gctcgtccgg caagagctgg tgaagccgat accgccacca ccgctcgtcg ccgagacacc
     5401 gcccgatcag accccgcccg agcaacagga gtcgaagccg atcgagcagc cggaaccggc
     5461 cgagcagccg cctcaggtac cggcgatcgc cgacgccgaa ccgtccgagc tgccgggcca
     5521 gctcggggcg gagacgccgt ccgtcgcggc ggcggcgcca gggaaaacgg tggagaaggc
     5581 cggcatcctc gcgttcaagg acgagttcgc cagcctggcg cgggacaacg tcgcgccgcg
     5641 cctgggagcc gatgcgcgct atggcgatgc cgccgacgcg agccgtgcat cgtcggcagc
     5701 gcgctcgatg ttgacgacca acgcgccggg ctcgagccgc ggcatcgacg tctcatcgct
     5761 gagtcgcagc gtgggcggcg gcggcggggg agacggcacc ggcgacggcg gcggcggcgg
     5821 aatgcaaggc gtgcaagtcg gccgcgcgag cagctcgatc gcttcgatcg gcggcggtgg
     5881 tcggccgctg gcgcgcggcg ggccggggcc ttcgcggacc gacgaagaga tccagatcgt
     5941 cttcgaccgc tacaaggcgt cgttctaccg gctgtacaac cgggagctgc gcaatgaccc
     6001 gaccttgcgc ggacagatgg tgctgcgcct gacgatcgag ccggacggca gcgtgtcgat
     6061 gtgcaagctg cagtcgaccg acatggatgc gccggcgctc gccgctcagg tcgtcgagcg
     6121 cgtgctgacg atcaacttcg gcgccaagga cgtcgaggcg ctgacgatcg tctacccgat
     6181 cgatttcctg ccggcggcgt gaacccggcg gctgaagaac aggggcatct gtggctctgc
     6241 gcaattcaat cggtgcgaag gcgggcggcg ggaggacgag cttcctgctg ctgctcttcg
     6301 cgctgctgct cggtgcgtcg tccgacgcgc aggcgatcga ttgcgtcgcc gatgccggcg
     6361 gggtcatcga cggattcgtc aattaccccg ttccgccacc gcagatcaat atcgacggcc
     6421 cttgcaccat ccggaactac accgcctcca acccgctgac cagcaacatc agctggttcg
     6481 gcaacaaccc cacgagctgg ctgctcatct tcgacaacgt cgtccacacc ggcaacatgt
     6541 cgtgcaacct cgcggctcag ggcaaccgga tctggttcgt caacagcgcg tccaccacgg
     6601 tccagtccaa ctgcctgagc ctgctgatcc cggtcgagaa gatcgacaag gcgaacccgc
     6661 cggggccgcc gttcgtcacc gtcggagtcc ccttcagctg gacgctgacg atcccggtcc
     6721 tgttcgaccc gggcaccggc accgtgatca acaaccaggg aagcgtcaac gacctgcaca
     6781 gcatcacggt ctgggacgac ctcaacgcga cgggggtgga cctgacctac gtgagccaca
     6841 cgctcacctg gctcgacgac ggcacccagg taccgcacac gttcaccaac gtcggcggct
     6901 tcctgacctt cgacaacatc cccgtcacga cggccgggcg gcagttcgtc atcgagctga
     6961 cggtcgtgct cgaagacacg ccggtgaacg cgccggggac gcagttcgtc aacacggcca
     7021 cgtgggactt cgggcggctc atcgagggcg tgttctacga gccactaccg ggagagaacg
     7081 gcatcagtcc gccgctgacg atcgccgcac cggtgctggt catggacaag acgggcccgg
     7141 cgacgatgaa cctcggacaa tcgggcgatt tcgtcctcga cgtccagaac accgggtcga
     7201 tcgacgcctg ggacgtcgcg ctgcgcgacc tgttcccgga cggcccgacc ggcgggatgt
     7261 gcgatctcca gccgcagatc gtgagcgcgc aggtgttcgc ggccgacggc atcacccccg
     7321 tgccgggcaa gggcccgctc gccgccggcg tcgactacac gacgacctgg ggcggcgcgc
     7381 ccagctgccg cctcgacctg acgatgctga cggccgcggc ccggatcggc ccgagcgagc
     7441 ggctgatcgt ccgctaccgg acccagctcg acgccgacac gctggacggc gccgcgctta
     7501 ccaatgtcgc cggcgcgatc gagtggttca acggcgccag cggcaacccc gagcgcgtcg
     7561 ccaccaaccg gaccctgacc aacggcacgg tcggcgtcca ggaccacgag gacgcccaca
     7621 ccgtgacggt ggcgctcacg ggctacttct tcgagaagac cgcggcgaac ctgaccagcg
     7681 gcgtcgatcc cacatcgacc gcggctcccg gcgacaccct gcgctacacg gtgaggttcc
     7741 gcgccaccga tcaggcgctc ggcgatttca gcatccatga cgagctggac gccctcaacg
     7801 cgccgccggc cttcgtgccc ggcacgctga cgctcgtcag ctatccgccg ggcgccgaca
     7861 tcagcggcac cagcagcacg ggcggcgccg acggcaccgg tgtcatcgac gtcggtaacc
     7921 tgagcgtgcc tctcgacggc gaggtggtga tcgagttcga catcacgctg gcctcgacac
     7981 tcgacaacgg cacggtcgtc accaaccagt cggcggtccg gctcacgagc ggcgcgacct
     8041 tcaccctgag cgacgacccg aacgtcaacg gtacggccga tcccctcgtc ccgggggacg
     8101 aagaccccac ccgggtgaca atcgtgtcgg cgccggactt ccgcattcac aagatctcga
     8161 ccgatctgac cggcgacccg aacgtcctgc tcgccggcga gacgctgcgc tacacgatca
     8221 cggtccaaaa cgtcggcaac gaagacgcac tcgacgtggt gctgcgcgac gcggtgccgg
     8281 tgaacacgac ctacgtcgcc ggcagcacga cgctgaacgg tgcggcggtc gcggacgtcg
     8341 gcggcctgtc cccgctggtc aacggcatgc tgatccactc gccggccgat ccgacgccgg
     8401 gctcgatgcc cgccgatgcc tcgggcaacc cggcgaacgt cgcgaccatc accttcgacg
     8461 tggtcgtcga tacggacgtc gtcgacggca cgatcatctc gaaccagggc ttcgtcagcg
     8521 cggtcgacgt cggcatcgtc gaccagccgt ccgacgaccc ggacacgccg atcgccgacg
     8581 acccgacgct cgacgtcgtc ggcaacctgc cgttgctcta cgccgaaaaa cgcgtcgctc
     8641 tgttcggcga cctgggctcg ccgggcatcg tcgatccggg cgatgtgctg cggtacacga
     8701 tcaccgtaca caattccgcg gcgattccgg cgaccggcat cgtcttgacc gactcggttc
     8761 cggcgaacac gacgtacgtc gccgactcca cgctgttgaa cggcttggcg gtcggccagc
     8821 cggacggcgg cgtcgccccg ctcgcctcgg gaatcgacat cagctcgtcc gacctgacgc
     8881 cgccgctgcc cggtccggga caaggcacga tctcgcccgg tgccaccgcg gtcctccagt
     8941 tcgacctgcg cgtcgacaac ggcacgccgg cgggcacgct gatcagcaac caggccgtgg
     9001 tcgacagcgt cgagctgccg gacctgttga ccgacggcga tggcgacccc gccacgggtc
     9061 ccgagccgac ggtcgtcgtc gtcggtgacg gccagctgct gtcgatcacc aagcaggtcg
     9121 ccgtggtcgg tggcggcccg gcggtccccg gcgcggaact cgaatacgtc gtgcgcgtcg
     9181 tgaacatcgc cgccgtgccg gccctcggcg tcgtcctcac cgacgacctc gacgcggtgc
     9241 aacccggcca gctgacctac gtcgacctat cggcgacgat gaacggttcg gcgacggggg
     9301 tcagcttcgc cggctcgacg atcaccgccg actacgacgc ggtcaacgga ccgctcgcgc
     9361 cgggcgaggc cgtcgtgctc cgcttccggg cgatcctcga tccggcgttg ttgaacggca
     9421 cggtggtgac gaacaccggc gtcgtcagct ggaacacgcc ggcacagacc gcgagcgcga
     9481 gcgtctcgat cgtcgtcggc ggcatccccg gcttcgccgc gctcaacgga tcggcctggc
     9541 acgacgccga cttcgacgat gcccgggatt ccaccgagcg cgcgctggcc ggctggtcgg
     9601 tcgagctgta cggcgatggc cagctgctgc attcggcgct gaccgacgcc aacggcgtct
     9661 atcgcatcgt aggcgtcgag ccgaacgacg tgagcggaac cgtctacgag ctgcggttca
     9721 gcgcgcccgg ctcgggcgcg agcaccgcga tgctgggcca ggccgcctcg ccgttcacga
     9781 acgggatgca acggatcggc gacatcctcg tgccgcccga cgccaacctg caggggctcg
     9841 acctgccgat cgatccgaac ggcgtcgtct acaactcggt cgcgcgcatt cccgtcgccg
     9901 gagcgacgct gaccctgctc gatgccggcg gcggctcgcc cttgccggcg aactgcttcg
     9961 acgacgcggc ccagcagggg caggtgacgc tcggggacgg ctactacaag ttcgacgtca
    10021 acttcagcga tccggcctgc ccgcccggcg ccggctacgt catcggcgtg acgccgccgc
    10081 ccggaacgac ctacgtcgcg ggctactcgc agatcatccc gccggcgtcc gacccttcga
    10141 ccgcagcgtt ctcggtgccg gcctgcccgg ggaccgtcga cgacaccgtt ccgagcacgg
    10201 cccagttctg cgaggtccaa ccgtcggagt tcgcgccggc ctcatcggtc gcggcacgcg
    10261 cggcgggcac ggtccaccac gtccacgtga cgctcgacgg cagccaagcg cccggctcga
    10321 gccagatctt caacaaccac ctcccgctcg accccgaggt caacggctcg atcgcgatct
    10381 cgaagacgac gccgctgctc tacgtgacgc gcgggcagca ggttccctac acgctgcggc
    10441 tgaacaacct ggccgggacg ttgctcaccg acgtgagcat cgtcgaccgc ttcccggccg
    10501 gcttcgccta cgtcgagggc tccgccgtcc tcgacggcgt gccgaccgag ccgaccgtcg
    10561 ccgggcgcga gctgagctgg agcggtctgg tcgtcgcggg caccgaggag cggacggtca
    10621 agctgctgct cgccgtcggc gccggtgtca gcgagggcga ctacgtcaac cgcgcgcagg
    10681 tgctgaacgg cgtcaccggc agcgcgatgt ccggtgaagc gaccgccacg gtgcacgtcg
    10741 tgcccgatcc gacgttcgat tgcaccgacg tgaccggcaa ggtcttcaac gacgcgaacc
    10801 gcaacgggat gcaggacgac ggtgaggacg gcctgcccgg cgtgcgcgtg gccacggcac
    10861 ggggcctgct cgcgacgacc gatcagcacg ggcggtacca catcacctgt gcgaccacgc
    10921 cgaacgagat ccgcggcagc aacttcgtgc tgaagctcga cgaccggacg ctgccgagcg
    10981 gcttccgcat gtcgaccgag ccggtgcgga tcaagcgtgc cacgcgaggc aaggtgttgc
    11041 ggatcaactt cggtgcgtcg atccaccggg tggtcggcat cgatctgtcc gatgcggcgt
    11101 tcgagccggg cacgaccgag attcgcgtcc agtggcgacc gcgcctcgac ttgctgctcg
    11161 acgagctgcg caaggcgccg tcggtgctgc gtctgtccta cgtcgccgac acggaggacg
    11221 cggcgctggt caaacggcgg atcgatgcgg tcgagcgaca ggtgaccgaa gcctgggacg
    11281 ccgcgagcca cggctaccgg ctcaccatcg agcccgagat cttctggcgc ctcggtgccc
    11341 cgtcgcggca gcccgacggt cgcctgtcgg acggtaggtg aacggcatgg gcacgcaccg
    11401 ccatctactg ctgatcgccg gcttgctgat cgccgtcccc gcgacggccg atccggtcga
    11461 aacgaccgag ccgggcgagt cggtcgagcg acatctgtcg gacgatcaga ccttccggca
    11521 atgggtgcag gacccggatc tgctcgacag cgagcggggc gatcgactgg aggttcggca
    11581 ggtcgtcggc gaggacctcg agaccgtcaa gctgaagaac gtcgtcccgc cgatccggtt
    11641 caagtcgggt gtggcggaga ttccaccgga ctacgtcgat cggttggcca aggtgctcgc
    11701 gagcatgcgc gatcgcagaa acgtccgcct ccatctcgtc gggcacgcgg actcgcagcc
    11761 gctgtccgac gcgctggtgc gcgtcttcgg cgacaacgcg ggtctgtccc gcgagcgcgc
    11821 cggcgaggtg gcggagtact tcaagaccgc gctcgccttg ccgcccgagg cgattaccta
    11881 cgagtgggcc ggcgacgcgc agccgatcgc atcgaacctg accgacgagg gccgcgcgct
    11941 caaccggcgg gtcgaggtcg aggtatggta tgacgaggtc cgggaggccc tgcgcgacga
    12001 agaggtcgtc gtctccgacg agatcaagcg aatcaaggtc tgccggatgg agaccgtctg
    12061 caagctgcgc taccgggaag gtcatgcgcg gcgggcgcgc gtccgtaacc tcgtcgtgcc
    12121 gctgcggtac gaagacgaaa ccacgccgat ctccgaggac ttcctccacc agattcggca
    12181 ggcgctggtc aacctcaggg acaagcggaa cgtggcggtg cggttcatcg gccacaccga
    12241 cgacgcgccg ctgaccggcc gtaacgagcg catctacgga gaccagctgt cgctgtcgaa
    12301 ggccagagcg ctccgcgtcg cgctggcgat ccaggaagcc ctgaccttgc cgacctcggc
    12361 gatcgagagc gacggtcgcg gcgcctcgcg gccgctcgcg tcgaacgaga ccgcgcaggg
    12421 gcgcaagctg aatcgtcgcg tcgaggtcga gttctggtac gacgacccgc tgcaggagct
    12481 gccggacgag ccgcaggtct gtcccggcga cgtcggcgag gaaatggtga ccaaggtcta
    12541 cgaccccccc tggggccgga tcccgacgct cgagctggtg gacggccggc cgatcgttcc
    12601 gcccggctac gcggcggacc tccatcgcgc gctgagcgac atcgcggatc ggaccaacgc
    12661 gcgactgcgc ttcatcggtt acacgaagaa cgagcgcctc gaccgccgca ccgcgtcggt
    12721 ctacggcgac gacatcggtc tgtccgccgc acgggcgcgt cgcacgatgg agacggtcaa
    12781 gcaggatccg ttgttgtcga gcgccccctc cgagcacgag ggacgcggct acgtccattc
    12841 cgacgacgtc gtcaacgccg ggttcatcca gggcgaaagg tcgttcgtgc gcgtacaggt
    12901 cgtctacgac gaaccgatgc cgctcgacga ctacgagggt gtcgacatca cccgcatcac
    12961 gcgagagctg cgtccgaaga gcgcctacga gctcaacgtg atgcgcatca ccgtcgacgg
    13021 caagccgatc gacgacccga atcgcagctc ctccgacgtc cagcgctgca ccgacgtcgc
    13081 gctcgacgac gcccggatcc gcttccactt cgacaatctc gcgtccgagc ggcggctcgc
    13141 cgtcgcggcg gatccggttg ccgtggcggt gagcgacccc ggggacggcc ccgtcgcatc
    13201 ggtcgtgcgt ttccggatgt acagcaacta ctcgagcttc atcgagcgtg ccgagatccg
    13261 aatcttcgag cagcagcagt cgttggacgc cgtgccgctc gaggtgatcg cgatcgacga
    13321 ctccggcctg gcggagtggc agccggtggt ggaaccgccg gccgccccga cccgcgagct
    13381 cgcgtacgtg ctgcgggtct acgactcgaa gggcaacttc gacgagacgg acgtgcggcc
    13441 gctgtggttg taccgcgagc cggcgcccga cgaagtcgtc ccggccgccg ccgagaccgg
    13501 tccgcctgac gagctgctgg cggcctacgg cgagagcgac gtcgagcgca gccggattcg
    13561 gctgggcggc agcggcacgg tcagggtgca gggcagcggc ataccggcgg gtcacacggt
    13621 gtgggtcgcg ggtcgccgag ttccggtcga cccgcacggc agcttcgtcg ccgaggaaat
    13681 cctgccgagc ggggcgcata cggtcgaggt cgcggtgctc gacgacgccg gcagcggctc
    13741 gctctatctg cgcgacctgg agttcaagcg caccgatctg ttctacctcg gcctcgccga
    13801 cctcacgctg tccgacacca gcgccaacgg cccggtcgaa atgctcgagg gccaggacgc
    13861 gccgcagccc ttcgactcgt cgatcgatgg ccggctagcg ttttttgcca acggcagggt
    13921 ccgcgagcac tggcaattga cggcgagcgc ggatacccgc gaaggtccgg tcgaggatct
    13981 gttcagcaac ttc
//
LOCUS       DASPGN010000003         4172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_703015, whole genome shotgun sequence.
ACCESSION   DASPGN010000003 DASPGN010000000
VERSION     DASPGN010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703015"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VD788_00085"
     CDS             complement(<1..468)
                     /locus_tag="VD788_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXV74688.1"
                     /translation="MLEPPRPRRWLARVRVGSVFGLLALLVVCARPTPLSVTLGFAVC
                     MTGEFIRIWAAGHLHKTVELVTSGPYRFTRNPLYLGRLLIFSGICVMSELPNRLHLVL
                     LALGWVVFFAYYLPRKERVERGRLRQLHGARFERYEQAVPALFPSPRPHLDRAS"
     gene            complement(510..1199)
                     /locus_tag="VD788_00090"
     CDS             complement(510..1199)
                     /locus_tag="VD788_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74689.1"
                     /translation="MKGSTATPPAVPAGRLRHAPVTRRIPGRNYTKGEITLRTLDGKR
                     FAVKDYRPRPFLMRQTLGRYLVGRECRAYHAAGRIDGLARYAGRVDRFALATEWVEGR
                     TLNGFAPGAQPADLFERLAGLLRALHERGVALADLHRRDVIVGPRGELTVVDLAAAWM
                     LKPGAARAARAAFVRLCCQDEIALARMRAHYHGEDEAAVLAALDPAAVARYRRARAVK
                     SFVDRVRRGGS"
     gene            1250..1975
                     /locus_tag="VD788_00095"
     CDS             1250..1975
                     /locus_tag="VD788_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74690.1"
                     /translation="MKETTTPTITPRRETGNHGRAPRLLPTREQLAGARLFRKGQRTK
                     ADLLRVETADGPLIVKDFGGKPRWVRHGGRWMIARELRAYRRLTGCLGVTRALGRVDR
                     YALALDYLDATQLAFSAQRTVDGAGKLAQLRAIVDGLHARGVVHCDLRSRDNVLVDPI
                     GRLYVVDFAAAVCLRPGSLVHRLLFARLAAIDRSACLKWKRILDAGPLTDDERAFVDR
                     FRAWRPFWIHRREAWRGKTRPPA"
     gene            1972..2751
                     /locus_tag="VD788_00100"
     CDS             1972..2751
                     /locus_tag="VD788_00100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV74691.1"
                     /translation="MNDAVVGLDLSIVAPVYNERPNLEPFVAEVEAALRDSGMSHEIL
                     LVDDGSTDGSSELIDRLAGQTTRVRAIHLRANRGQTAAFDAGFKYARGRIVVTIDADL
                     QNDPRDIIGLVAELERYDAAVGYRRTRQDRWLRRVSSTIANRVRNSLSGDDIIDTGCS
                     LKAFRRETLSSLKLYTGMHRFLPTLLRIEGYRVVQRPVAHRPRHAGHSKYGVRNRAVR
                     AFVDLLAVRWMKRRHLGYEIVTPQATDQRDESSPASSSRAK"
     gene            complement(2706..3947)
                     /locus_tag="VD788_00105"
     CDS             complement(2706..3947)
                     /locus_tag="VD788_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV74692.1"
                     /translation="MSKRISLALVASASFSGALAAGTLEFETPRHLSTVLGPTEIRLR
                     LSDVDASTAEQMVIRVDGQLVASLTRPPWTAAFDAGDGTRGHRIEAILSLTDGSVLRA
                     AVRTSPLRIDETLDVDLVSLYPLVLDPAGGYVRGLSAEDFAVLEDGQPQRIQRFTTEQ
                     RPIRLAFVIDTSLSMSKGGRLASAKSAALGFLEVLSDRDDALVVAFADRVELLQDVTQ
                     DRVQLGAAIESTTANGGTALYDAIWRTCRRLEDFDGRRVMVLLSDGRDEAANGFEPGS
                     LHTLEEARLQAVKSEVMIFAIGLGRDLDSEFAREWTRSGVLDTSGSRVSLKRILETLA
                     VHSGGRLLLTQSASKLRRAFAAVADDLRNQYSLAYTPSNDRDDGSYRKIEVTVPGRQV
                     EVVVRKGYFARDEEAGDDSSR"
BASE COUNT          676 a         1420 c         1450 g          626 t
ORIGIN      
        1 ggatgcgcga tcgaggtgtg gccgcgggct gggaaacagc gccgggaccg cttgctcgta
       61 tctctcgaaa cgcgcgccgt gcagctgccg cagccggcca cgttcgaccc gttccttgcg
      121 cggaaggtag taagcgaaaa agacgaccca gccgagggcg agcaggacga gatggagcct
      181 gttcggcagc tcgctcatca cgcagatccc gctgaagatc aacaatcgcc cgagatagag
      241 tgggttgcgc gtgaagcggt aggggcccga tgtcaccagc tcgaccgtct tgtgcagatg
      301 gccggcggcc cagatccgga tgaactcgcc ggtcatgcaa acggcgaagc cgagcgtgac
      361 ggacagtggc gtcggccgtg cgcaaaccac gagcaacgcc aacagaccga agaccgagcc
      421 gacgcggact cgcgcgagcc agcgacgcgg tcgcggtggt tcgagcatga cgcccagcat
      481 agctcagtgc cgatcgcccg gcaccgacct cagctcccgc cgcgacgcac gcggtcgacg
      541 aacgacttga ccgcacgcgc gcgacggtag cgggcgaccg cagccggatc gagcgcggcg
      601 agcacagcgg cttcgtcttc accgtggtaa tgggcgcgca tccgcgcaag cgcgatctcg
      661 tcttggcagc agaggcgcac gaacgccgcg cgggccgcgc gcgcggcacc gggcttcagc
      721 atccacgccg ccgccaggtc aacgacggtc agctcgccgc gcgggccgac gatcacgtcc
      781 cgccggtgca ggtcggccaa ggcgacgcct cgctcgtgca gcgcccgcag caagccggcc
      841 aaccgctcga acagatcggc gggttgcgcg cccggcgcaa agccgttcag cgtacgaccc
      901 tccacccatt cggtcgccag tgcgaagcgg tcgactcggc ccgcgtatcg cgcgagcccg
      961 tcgatccgcc ccgcggcgtg gtacgcgcga cactcgcgtc cgacgaggta gcgccccagt
     1021 gtttggcgca tcagaaacgg gcgtggccga tagtccttga cggcgaaccg cttgccgtcg
     1081 agcgtgcgaa gagtgatctc gcccttggtg tagttgcgac cggggatgcg tcgcgtgacg
     1141 ggggcgtggc gcagccggcc ggcaggcacg gcgggcggcg tcgctgtgct acccttcacc
     1201 cgaacctcga accgtgtcgt gacgccgcgc catctccgag aagacgcgtt tgaaggaaac
     1261 gacgacccca acgataacac cgcgccggga gacgggcaat cacggtcgtg ccccgcggct
     1321 gctgccgacg cgagagcagt tggccggtgc tcggctgttt cgcaagggac agcgaacgaa
     1381 ggccgacctg ttgcgagtcg aaacggccga cggaccgctg atcgtcaagg attttggcgg
     1441 caagccgcga tgggttcgtc acggcggacg atggatgatc gcacgggagc tccgcgccta
     1501 ccgccgattg accggctgcc tcggcgtcac ccgcgcgctc ggccgggtcg atcgttacgc
     1561 gctcgcgctc gactacctcg atgcgaccca actcgccttc tccgcacagc gcacggtcga
     1621 cggtgcgggc aagctggctc aattgcgggc gatcgtcgac gggctgcacg cgcggggagt
     1681 ggtgcattgc gatctgcgct cgcgcgacaa cgtgctcgtc gacccgatcg gccgattgta
     1741 cgtcgtcgac ttcgccgcgg cggtctgcct gcggccgggc tcgctcgtgc atcgtctgtt
     1801 gttcgcccgt ttggccgcga tcgaccgctc ggcctgtctc aagtggaagc ggatcctcga
     1861 cgccggcccg ctgaccgacg acgaacgggc gttcgtcgat cggttccgcg cgtggcggcc
     1921 gttttggatc caccgacgcg aggcctggcg cggcaagacg agaccgcccg catgaacgac
     1981 gcggtcgtcg ggctcgatct gagcatcgtc gctccggtct acaacgagcg ccccaacctc
     2041 gagccgttcg tcgccgaggt cgaggcggca ttgcgcgatt ccggcatgag ccacgagatc
     2101 ctgctggtcg acgacggcag caccgacggc tcctcggagc tgatcgatcg attggccggg
     2161 cagacgaccc gcgtccgtgc gatccatcta cgggcgaacc gtggccaaac cgcggcgttc
     2221 gacgcggggt tcaagtacgc gcgcggcagg atcgtcgtca cgatcgacgc ggatctgcag
     2281 aacgacccgc gcgatatcat cggcctggtg gccgagctcg agcgttacga cgcggccgtc
     2341 ggctaccgcc gtacgcggca ggaccgttgg ctgcgtcgcg tttcctcgac gatcgcgaat
     2401 cgcgtgcgca acagtctgag cggtgacgac atcatcgaca ccggctgttc gctcaaggcg
     2461 tttcgacgcg agacgttgtc gtccctcaag ctgtacactg gcatgcatcg gttcctgcct
     2521 acgttgttgc gcatcgaggg ctaccgcgtc gtccaacggc cggtggcgca ccgcccgcgg
     2581 cacgccggcc acagcaagta cggcgtgcgc aaccgcgccg tgcgtgcctt cgtcgacttg
     2641 ctggcggtgc gctggatgaa acgacgccac ctggggtacg agatcgtcac cccgcaggcg
     2701 acggatcagc gcgacgagtc gtcgccggcc tcctcgtccc gcgcgaagta gcccttgcgc
     2761 acgacgacct ccacctggcg gcccggcacc gtcacctcga tcttgcggta gctgccgtcg
     2821 tcgcggtcgt tgctcggggt gtaggccaac gagtactggt tccgcaggtc gtcggcgacg
     2881 gcggcgaacg ctcgccgcag cttgctcgcg ctctgcgtca gcagcaggcg gccgccggag
     2941 tgcacggcga gcgtctcgag gatccgcttc aacgagacgc gtgacccgct ggtgtcgagc
     3001 actcccgacc gagtccactc gcgtgcgaac tcgctgtcga ggtctcgccc gaggccgatg
     3061 gcgaagatca tcacctcgct cttgaccgct tgcagtcgag cttcctccag cgtgtgcagg
     3121 ctgcccggtt cgaacccgtt ggccgcctcg tcccgcccgt ccgacagcag gaccatcacc
     3181 cggcggccgt cgaaatcctc cagccggcgg caggtgcgcc agatcgcatc gtagagcgcc
     3241 gtgccgccgt tggccgtggt cgactcgatc gcggcgccga gctgcacccg gtcctgcgtg
     3301 acgtcctgca gcaactcgac gcggtcggcg aatgccacga ccagcgcgtc gtcgcgatcg
     3361 ctgagcacct cgagaaaacc gagggcggcg ctcttcgcgc tggccaggcg accgcccttc
     3421 gacatgctga gcgacgtgtc gatgacgaac gccaggcgga tcggacgctg ctcggtggtg
     3481 aagcgttgga tccgctgcgg ctgcccgtcc tcgagaacgg cgaagtcctc ggcggacaga
     3541 ccgcgaacgt agccgccggc cgggtcgagc acgagtggat agagactgac caggtcgacg
     3601 tcgagtgtct cgtcgatgcg caacgggctg gttcggaccg cggcacgcag gacgctgccg
     3661 tcggtcagcg agaggatggc ttcgatccgg tgaccgcggg tgccgtcacc ggcgtcgaac
     3721 gccgcggtcc acggggggcg ggtcagcgag gcgacgagct gaccgtcgac gcggatgacc
     3781 atctgctcgg cggtcgaggc gtcgacgtcg gacaggcgca ggcgaatctc ggtcggcccg
     3841 agcacggtcg acaggtgccg cggggtttcg aactcgaggg ttccggctgc gagcgcgccg
     3901 ctgaacgaag cagatgccac gagtgccagc gatatacgct tcgacacggc ccgggatcga
     3961 caggtctggc gaccattcat cgcgtgacat catatcgggc gcgatgaccg ttcgcccgtc
     4021 ggcttcgccg ccgatcaggg gtggggttgg gccggcagcc gcagaccgtt cgccggcacc
     4081 gctggggtgc ccgatcgcgt gccggcgcgc gtgagcagga acaggccgac gccgggtgcc
     4141 tcgaccagga cgaagaagac cgcgcggaag gc
//
LOCUS       DASPGN010000004         7506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_614, whole genome shotgun sequence.
ACCESSION   DASPGN010000004 DASPGN010000000
VERSION     DASPGN010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7506
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1946
                     /locus_tag="VD788_00110"
     CDS             33..1946
                     /locus_tag="VD788_00110"
                     /inference="COORDINATES: protein motif:HMM:NF005749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HXV74693.1"
                     /translation="MQLNPHVPAGPLEQKWDKHKAEILLVNPANKRKFEVIVVGTGLA
                     GASATASLAELGYNVSAFCFHDSPRRAHSIAAQGGINAAKNYQADGDSVFRLFYDTVK
                     GGDFRAREANVYRLAEISVEIIDQCVAQGVPFAREYGGLLANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALERQIAAGKVEMFNRHEMLDLIVIDGRARGIVTREMTSGAVASHV
                     ADAVVLATGGYSNVFYLSTNAKACNTTAIWRAFRRGAGFANPCFTQIHPTCIPSSGEY
                     QSKLTLMSESLRNDGRIWVPKRRGDRRAPGEIPESERDYYLERFYPSFGNLSPRDISS
                     RRAKQMVDEGHGVGPQANGVYLDFADAIDRLGRRLIAERYGNLFDMYARITGENPYEV
                     PMRIYPAPHYTMGGLWVDYNLMSTVPGLFVIGEANFSDHGANRLGASALMQGLADGYF
                     ILPYTIGDYLARHAPDGIGADHAACTTVEADVRRRLEALLTSGGKRPSTGLHRELGRV
                     MWEHCGMARNARGLGAAIEALRELRPRFWREVRVPGAGEDVNQALETAGRVADFFELA
                     ELMCLDALQRDESCGAHFREDHQTSEGEAQRDDEHFSHVTVWQYTGEDQPPRSWLEPL
                     HFDDVTPTQRSYK"
     gene            1946..2683
                     /locus_tag="VD788_00115"
     CDS             1946..2683
                     /locus_tag="VD788_00115"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HXV74694.1"
                     /translation="MKLKLHVWRQPSRSAQGRFAEYEAQEISEHASFLEMLDLVNERL
                     IAAGEEPIAFEHDCREGICGSCGMMVNGVPHGPHEGIAACQLHMRSFADGDEIWIEPW
                     RATAFPIVRDLVVDRSSLDRVIQAGGFISINTGNAQDANAVPIPKDVADTAMDAAACI
                     GCGACVAACPNASAMLFTAAKVGHLNALPQGQPERRRRVVGMVARMDQEGFGNCTNHG
                     ACERACPKQISIEFIARLNRDLLKASF"
     gene            complement(2680..3387)
                     /locus_tag="VD788_00120"
     CDS             complement(2680..3387)
                     /locus_tag="VD788_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HXV74695.1"
                     /translation="MAKPNLSEIVERQARVWEVRERIAREGGEAARRALAHLAEGPWI
                     SLSKQWGCGARAIAAKLAEALHWQWFDKEIVAAIAEHSHTLQSIVSRLDEHAIGTIND
                     YLAQMTVPNDPGQVNYLREMVRVVSGLAHNGNAIILGRGANFFLKPQFGLRVRLVAPL
                     EARVRRLVEEREVDEKTARAAIKTQDDGQRAFVRQVFRADVDDATGYDLVVNTADLGL
                     DVAARVIEAALRRKLQA"
     gene            complement(3502..4425)
                     /locus_tag="VD788_00125"
     CDS             complement(3502..4425)
                     /locus_tag="VD788_00125"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXV74696.1"
                     /translation="MSEPVSRFPVGRRDTGKRLDRFLHERIPGLSRTRIQRVIRARVR
                     LSWGATPRPATPVRAGEEVTIVPRPRDEVVRDLRLDILARGDGWLAVDKPPRIPVHPV
                     HDTVDNSVIRLLRRQLDDTQLRLVHRLDAETSGVLLVARTVEASRRLARAFMERTVGK
                     HYLALVRGNPVADGGTIDLPIGPARGSRVWIRLEAGHGKPARTDWRVARRFGERTLVR
                     LSPATGRRHQLRVHMTALGHPILGDILYGRTDDDYLRMVRGEGDARASGGPRRQLLHC
                     SRLEFEDPGSGARCAVSAALPADFVEQLAAR"
     gene            complement(4422..4859)
                     /locus_tag="VD788_00130"
     CDS             complement(4422..4859)
                     /locus_tag="VD788_00130"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HXV74697.1"
                     /translation="MAGSPRSGAGSPFEETAGRVDIALAVVLRDGRFLVARRQPGRHL
                     AGRWEFPGGKIGAGEPPADAARRELREETGLDAGVLEPLAVVVHDYADRPLRFHVYLV
                     REPSGEPRWDGEREWGWKTLDELTGLDMPEANRQMLRALRWRL"
     gene            complement(4861..5715)
                     /locus_tag="VD788_00135"
     CDS             complement(4861..5715)
                     /locus_tag="VD788_00135"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HXV74698.1"
                     /translation="MRIDKIGVVGAGQMGNGIAHVAAQAGLAVRLHDVSDERVAAGLR
                     AIDNNLQRAVDRGKLAAGDKAAVLARIDSTTELSRFADCDLVVEAIVERFETKAAVFA
                     ELDRVCRRGVILASNTSSISITKLGGCTERPAQVIGMHFMNPVPVMKLVEVIRGLATA
                     GETTRAVVELAERMDKTAVEVADSPGFVSNRVLMPMINEAIFCLYEGVGTAESIDQVM
                     KLGMNHPMGPLTLADFIGLDTCLAILEVLYEGFADPKYRPCPLLVKHVEAGWLGRKTG
                     RGFYDYRS"
     gene            complement(5735..6916)
                     /locus_tag="VD788_00140"
     CDS             complement(5735..6916)
                     /locus_tag="VD788_00140"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXV74699.1"
                     /translation="MKRPVILSAVRIPTGRFIGSLAPLSATRLGQLVVAEAVRRAGLA
                     PQQVDEVIMGNVVGAGLGQNPARQAALGAGLPDTVAAMTINKVCGSGLKAVVLAAQAI
                     KSGDAELIVAGGMESMSNAPYLLPGAREGYRMGSNSVVDSVIHDGLWDAYHDYHMGNT
                     GEVVAERFHVSREQQDQWALDSHRKAIAAIDAGEFKREILAVEIPARKGDPLLFDTDE
                     PPRRETTIEALRKLKPAFKKDGTVTAGNAPGLSDGASALLIASAERAAELGLTPRAEI
                     VGYATSGLAPELVMMAPEKAIRRVWEKSGWSNDDVDLYEINEAFAVQQVALARELGID
                     PDKHNVRGGGVALGHPIGASGARILTTLLHALEDRGQRRGIAALCLGGGNAVALGIEL
                     S"
     gene            complement(7026..7367)
                     /locus_tag="VD788_00145"
     CDS             complement(7026..7367)
                     /locus_tag="VD788_00145"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HXV74700.1"
                     /translation="MTDCLFCRIVAGTIRSKKVYENDEILAFHDANPQAPTHILVIPK
                     PHIESLDAVRDGDAELLGRLLLAIRDLAAEAGIAAGYRLVSNCGAPAGQSVFHLHFHL
                     LGGRPLGWPPG"
BASE COUNT         1167 a         2665 c         2520 g         1154 t
ORIGIN      
        1 cttacttccc tatccggtcg cgggtttctg acatgcagct gaacccacac gtcccggccg
       61 gcccgctcga gcagaagtgg gacaagcaca aggcggaaat cctgctcgtc aatccggcca
      121 acaagcgcaa gttcgaggtc atcgtcgtcg gcaccggttt ggccggcgct tcggcgaccg
      181 cctcgctcgc cgagctcgga tacaacgtgt ccgctttctg ctttcacgac agcccgcggc
      241 gcgcgcacag catcgccgcg cagggcggca tcaacgcagc caagaactac caggccgacg
      301 gtgacagcgt ttttcgcctc ttctacgaca cggtgaaggg cggcgatttc cgcgcgcgcg
      361 aggcgaacgt gtaccgcctg gccgagatca gcgtcgagat catcgaccag tgcgtggcgc
      421 agggtgtacc gttcgcccgc gagtacggcg gactgctcgc caaccggtcg ttcggcggcg
      481 cgcaggtctc gcgcacgttc tacgcgcgtg gccagacggg acaacaactg ctgctcggcg
      541 cctaccaggc gctcgagcgg cagatcgccg cgggcaaggt cgagatgttc aaccggcacg
      601 agatgctgga cctgatcgtg atcgacgggc gcgcgcgtgg catcgtcacc cgcgagatga
      661 cgagcggcgc ggtcgcctcg cacgtcgccg atgcggtcgt gctggcgacc ggcggttact
      721 cgaacgtctt ctacctgtcg accaacgcca aggcctgcaa cacgaccgcg atctggcggg
      781 cgtttcgtcg cggggccggc ttcgccaacc cgtgcttcac ccagattcac ccgacctgca
      841 tcccgtcgtc cggcgagtac cagtcgaagc tgacgctgat gagcgagtcg ctgcgcaacg
      901 acgggcgcat ctgggtgccg aaacgccgag gcgaccggcg ggcaccgggc gagatccccg
      961 agtccgagcg cgactactac ctcgaacggt tctaccccag cttcggcaac ctcagcccgc
     1021 gcgacatctc ctcgcgccgc gcgaagcaga tggtcgacga aggtcacggc gtcggaccgc
     1081 aggccaacgg cgtctatctc gacttcgccg acgcaatcga ccggctgggc cgccggctga
     1141 tcgccgagcg ctacggcaac ctgttcgaca tgtacgcgcg gatcaccggc gagaacccgt
     1201 acgaggtgcc gatgaggatc taccccgcgc cgcactacac gatgggcggg ctgtgggtcg
     1261 attacaacct gatgagcacc gtaccggggc tgttcgtcat cggcgaggcg aacttttccg
     1321 atcacggcgc caaccgtctg ggtgccagcg ccttgatgca ggggctggcc gacggctact
     1381 tcatcctgcc gtacaccatc ggcgactacc tggcacggca cgcgccggac gggatcggag
     1441 cggaccacgc ggcgtgtacc accgtcgaag ccgatgtgcg gcggcgactc gaagccctgc
     1501 tgacctcggg cgggaagcgg ccgtcgaccg ggctgcatcg cgagctcggt cgcgtgatgt
     1561 gggagcattg cgggatggcg cgcaacgcgc gaggtctcgg cgcggcgatc gaggcgcttc
     1621 gggagctgcg accgcgcttc tggcgggagg tgcgcgtccc cggtgcggga gaagacgtca
     1681 accaggcgct cgaaacggcg gggcgggtcg ctgacttctt cgagctggcc gagctgatgt
     1741 gcctcgacgc tttgcaacgc gatgaatcat gcggagcgca ctttcgcgaa gaccaccaga
     1801 ccagcgaggg cgaggcgcaa cgcgacgacg agcacttctc tcacgtcacc gtttggcagt
     1861 acaccggcga ggatcaaccg ccgcgctcgt ggctggaacc gctgcacttc gacgacgtga
     1921 cgccgacgca gaggagctac aagtgatgaa gctcaagctc cacgtctggc gccagccgag
     1981 ccggtcggcg caggggcgat tcgccgaata cgaagcgcag gagatcagcg agcatgcgtc
     2041 gtttctcgag atgctggacc tggtgaacga gcggctgatc gccgcgggcg aggagccgat
     2101 cgcgttcgag cacgactgcc gcgagggtat ctgcggctcg tgcgggatga tggtcaacgg
     2161 cgtcccgcac gggccgcacg agggcatcgc cgcgtgccag ctgcacatgc gcagcttcgc
     2221 ggacggcgac gagatctgga tcgagccgtg gcgcgccacg gcattcccga ttgtccgcga
     2281 tctggtcgtc gaccgcagct cgctcgaccg ggtgatccag gcgggcggct tcatctcgat
     2341 caataccggc aacgcgcagg acgcgaacgc ggtgccgatc cccaaggacg tcgccgatac
     2401 cgcgatggac gcggcggcct gcatcggctg cggggcgtgc gtcgccgcct gtccgaacgc
     2461 ctcggcgatg ctgttcaccg cggccaaggt cggtcacctc aacgcgctgc cccagggtca
     2521 acccgagcga cggcgacggg tcgtcggcat ggtggcgcgg atggaccagg agggattcgg
     2581 caactgcacc aaccacggcg cgtgcgaacg ggcctgcccg aaacagatca gcatcgagtt
     2641 catcgcccga ctgaaccgcg acctgttgaa ggcgtcgttc taggcctgga gcttgcgtcg
     2701 cagtgcggcc tcgatcacgc gggccgcgac gtcgagcccc agatcggccg tgttgaccac
     2761 cagatcgtag cccgtcgcgt cgtcgacgtc ggcgcggaac acctggcgga cgaatgcccg
     2821 ctggccgtcg tcctgggttt tgatcgcggc acgcgcggtt ttctcgtcga cctcccgctc
     2881 ctcgaccagc cgacgcacgc gcgcctcgag cggcgcgacg agacgcacgc gcaagccgaa
     2941 ttgcggcttg agaaagaagt tggcgccgcg accgaggatg atcgcgttgc cgttgtgcgc
     3001 caggccgctg acgacccgca ccatctcccg caggtagttc acctgacccg ggtcgttcgg
     3061 caccgtcatc tgcgccaggt agtcgttgat cgtgccgatc gcatgctcat ccaaccgcga
     3121 gacgatcgac tgcagcgtgt ggctgtgctc ggcgatcgcg gcgacgatct ccttgtcgaa
     3181 ccactgccag tgcagcgcct cggccagctt ggccgcgatg gcgcgcgccc cgcagcccca
     3241 ctgcttggac agggagatcc acggtccctc ggccaggtgc gcgagcgccc gccgagcagc
     3301 ctctccgccc tcacgcgcga tccgctcgcg cacctcccag actcgcgcct gacgctcgac
     3361 gatctcgctc aggttcggct tggccaccgc gacacctgcc ttcccctgtc cagtgtagcg
     3421 cgccgcgggc accgcggcag cggcggccga ccgcgtccga agacgggtgc ggggcgatcg
     3481 ggcaccacgc cggcccgcgg cctaccgcgc cgcgagctgc tcgacgaaat cggcgggcag
     3541 cgccgcggag accgcgcacc gcgcgccgct ccccggatcc tcgaattcca atcggctgca
     3601 gtgcagcagc tggcgtcgcg gtccgccgga ggcgcgggcg tcgccctcgc cgcgcaccat
     3661 tcgcaggtag tcgtcgtcgg tccgcccgta gaggatgtcg cccaggatcg ggtgcccgag
     3721 cgcggtcatg tggacccgta gctggtgacg gcgtcccgtc gcgggcgaca gccggaccag
     3781 ggtccgttcg ccgaagcgac gcgcgacgcg ccagtcggtg cgggccggct tgccgtggcc
     3841 ggcttcgagc cggatccaca cccgcgaccc gcgcgccggg ccgatcggca ggtcgatcgt
     3901 cccgccgtcg gcgaccgggt tgccgcgcac cagtgccagg tagtgcttgc cgaccgtcct
     3961 ctccatgaac gcgcgggcca ggcggcgcga cgcttcgacg gtgcgggcca ccagcagcac
     4021 gcccgaggtc tcggcgtcga gacggtggac cagcctcagt tgcgtatcgt cgagctgtcg
     4081 gcgcagcagg cggatcacgc tgttgtccac cgtatcgtgg actggatgga ccgggatccg
     4141 cggcggcttg tcgacggcga gccagccgtc gcctcgggcg agaatgtcga ggcgtaggtc
     4201 gcggaccacc tcgtcacgcg ggcgcggaac gatcgtgacc tcctcccccg cgcgtaccgg
     4261 cgtcgccggc cgcggagtcg cgccccacga caggcggacc ctcgcgcgta tcacccgctg
     4321 gatgcgtgtc cgagacaggc cgggtatgcg ttcgtggagg aaccggtcca gtcgcttgcc
     4381 cgtgtcgcgc cgcccgacgg ggaagcgcga gaccggctcg ctcacaaccg ccaccgcagg
     4441 gcacgcagca tctgccggtt ggcctccggc atgtcgaggc cggtcagctc gtcgagcgtc
     4501 ttccagcccc actcgcgttc gccgtcccac cggggctcgc cgctcggctc gcggaccagg
     4561 taaacgtgga accgcagcgg ccgatcggcg tagtcgtgga cgaccacggc caacggctcg
     4621 agcacgccgg cgtcgagccc ggtctcttca cgcagctcgc gacgagcggc gtcggccggc
     4681 ggctcgcccg ccccgatctt gccgccggga aattcccagc gcccggcgag gtgccgaccc
     4741 ggttggcggc gcgcgacgag gaagcgaccg tcacgcagca cgacggcgag cgcgatgtcg
     4801 acgcgcccgg ccgtttcctc gaacggcgag cccgctccgc ttcgcggcga ccccgccatc
     4861 tcaggatcga tagtcgtaga acccgcgacc ggtcttgcgt ccgagccacc cggcttcgac
     4921 atgcttgacc aacagcgggc acgggcggta cttcgggtcg gcgaaaccct cgtacagcac
     4981 ctcgaggatc gccagacagg tgtcgaggcc gatgaagtcg gcgagcgtca gcggccccat
     5041 cgggtggttc atcccgagct tcatcacctg gtcgatcgat tccgccgtgc ccaccccctc
     5101 gtacagacag aagatcgcct cgttgatcat cggcatcagc acgcggttgc tgacgaaccc
     5161 cggcgagtcc gcgacctcga ccgccgtctt gtccatccgc tcggccagct cgaccacggc
     5221 ccgagtcgtc tcgcccgcgg tggccaaccc gcgaatcact tcgaccagct tcatcaccgg
     5281 aaccgggttc atgaagtgca tgccgatcac ctgtgccggc cgctcggtgc acccgccgag
     5341 cttggtgatc gagatcgacg acgtgttcga ggccaggatc acgccccggc ggcagacccg
     5401 atcaagctcg gcgaagaccg ccgccttggt ctcgaaccgc tcgacgatcg cctcgacgac
     5461 gaggtcgcaa tcggcgaagc gcgacaactc ggtcgtcgag tcgatgcgtg ccagtacggc
     5521 ggccttgtcg cctgccgcga gcttgccacg gtcgaccgcg cgttgcaggt tgttgtcgat
     5581 cgcgcgcagt ccggccgcga cgcgctcgtc gctgacgtcg tgcagtcgga cggccaaacc
     5641 ggcttgcgcg gcgacgtgcg cgatgccgtt gcccatctgt ccggcgccga ccacgccgat
     5701 cttgtcgatt cgcatctcgc ttcccccgtt cggctcagct cagttcgata cccagcgcca
     5761 cggcgttgcc gccgccgagg cacagcgcgg cgatcccgcg acgctgcccg cgatcctcga
     5821 gcgcatggag cagggtggtc aggatccgcg ctccgctggc gccgatcggg tggccgagcg
     5881 ccaccccgcc gccccgcacg ttgtgcttgt ccgggtcgat gcccaactcg cgcgcgagcg
     5941 ccacctgctg cacggcgaac gcctcgttga tctcgtacag gtcgacgtcg tcgttcgacc
     6001 aaccgctctt ctcccacacg cggcggatcg ccttctccgg tgccatcatc accagctccg
     6061 gcgccaggcc gctcgtcgcg tagccgacga tctcggcgcg cggggtcagc cccagctcgg
     6121 ccgcccgttc ggcggaggcg atcaagagcg ccgaggcacc gtcgctcaac ccgggcgcgt
     6181 tgcccgcggt caccgtcccg tccttcttga acgccggctt cagcttgcgc agcgcctcga
     6241 tcgtcgtctc gcgtcgcggc ggctcgtcgg tgtcgaacaa cagcggatct cccttgcgcg
     6301 ccggaatctc gacggccagg atctcgcgct tgaactcgcc ggcgtcgatc gccgcgatcg
     6361 ctttgcggtg gctgtcgagc gcccactggt cttgctgctc gcgcgagacg tggaagcgct
     6421 cggcgacgac ctcgccggtg ttgcccatgt ggtagtcgtg gtaggcgtcc cacagtccgt
     6481 cgtgaatcac cgagtcgacg acgctgttcg accccatccg gtacccctcc cgtgcaccgg
     6541 gcagcaggta gggcgcgttg ctcatcgatt ccatcccgcc tgcgacgatc agctccgcgt
     6601 cgcccgactt gatcgcctgc gccgcgagca cgacggcctt cagaccgctg ccgcacacct
     6661 tgttgatcgt catcgcggcg acggtatccg gcaggccggc ccccagcgcg gcctgccggg
     6721 ccgggttctg gccgagaccg gcgccgacga cgttgcccat gatcacctcg tcgacctgct
     6781 gcggcgcgag accggcgcga cgcaccgcct cggcgacgac aagctgcccc aggcgggtcg
     6841 cggagagcgg cgcgagcgac ccgatgaacc tcccggtcgg gatgcggact gcggagagga
     6901 tgacggggcg tttcatcagc ggaccctcct ggcatcagcg gcatggcggg gaaacgcagg
     6961 aattctatcc gccgtgcgac ggaccaacaa ggcggccaat cgacccgaac gacccgttgg
     7021 gatctttatc ccggcggcca gcccagcggc cgcccgccga gcaggtggaa gtgcaggtga
     7081 aacacgctct ggccggcggg cgcaccgcag ttgctgacga gccggtagcc ggcagcgata
     7141 ccggcctcgg cggcgagatc gcggatggcg agcagcagcc gtccgagcag ctcggcgtcg
     7201 ccgtcgcgca ccgcgtcgag cgattcgatg tgaggtttcg gaatgaccag gatgtgcgtc
     7261 ggtgcctgcg ggttggcgtc gtggaaggcg aggatctcgt cgttctcgta gaccttcttc
     7321 gaccggatcg ttccggcgac gattctgcag aacagacagt cggtcatcgg atcctttcaa
     7381 ccgaggcgcc caggcccgcc agcttggact ccatcgattc gtagcctcga tccaggtggt
     7441 agatacgatc gacgaccgtc gcgccctcgg cgaccagcgc ggccagcacc agaccggccg
     7501 acgccc
//
LOCUS       DASPGN010000005        11080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1522878, whole genome shotgun sequence.
ACCESSION   DASPGN010000005 DASPGN010000000
VERSION     DASPGN010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11080
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522878"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VD788_00150"
     CDS             complement(<1..459)
                     /locus_tag="VD788_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74701.1"
                     /translation="MFCPVCKDEFRPGFTECAGCGVALVERSDVGPAAGRGGRVSEAA
                     AARLHPPMVDYCGFLTLDEARDARSRLGRHRVRCEIVIRESTGSPGHVAVDEEYWLRV
                     ERDRYKEVIDHLGFDAADSTPPDETFTCGECGSEVASEESFCPDCGARFED"
     gene            656..1231
                     /locus_tag="VD788_00155"
     CDS             656..1231
                     /locus_tag="VD788_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV74702.1"
                     /translation="MSDQRDGETPVQHALEAKAATEPDLERVYRDHHRRVLQAAYRVT
                     GSAQDAEDVLQTVFLRLVRREGSSPLSDSLGNYLHRAAVNAALDVVRSRKASRSTPLD
                     SVEPTLAAGSETTPDAIQSEVEIKQRIRTALARMSAKAGEIFVLRYFEGYGNHEIATM
                     LGTSRSTVNVILHRTREKLRGELASYLGETP"
     gene            1228..3984
                     /locus_tag="VD788_00160"
     CDS             1228..3984
                     /locus_tag="VD788_00160"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXV74703.1"
                     /translation="MNRNADDFDRELDQALDRIRRDELPEATRQAISERVWARVGSAI
                     ADAAPDESEPHRIRDCSDFQALIPAYLRGGLNEAKSLLLSDHVTSCVPCRKTLIQARD
                     ARRRARAATAGATEPGWAARWSWRLAAAAVIVVALVGLSVKTDLFTVRTGGVIFIEAI
                     DGDVFQITGDGPVPLQVGDSLSFEDGRLIRTGRDSGAIFRTSDDSVVEVSERAELSVQ
                     DRRKLWNVRNPDSVIELNRGSVIVEASEQGSGHLYVDTPDAMISVTGTVFAVNRGIKG
                     SRVSVLEGEVAVNHSGGTHVLHPGEQATTQPGLGQVPVEDEIAWSRQLDKHLALLREF
                     AELGREIDRAVVPPNLRYGTALLDRVPDDTFVYVAIPNLSSTISQAYDLMRDKISGNG
                     LLNQWWEESIVTSGADAQIELAMEKIRSYGPQIGEEIVVTVGGIDRAHGDPSVLILAE
                     LVEPEAFLQMVRTDLTGLRLLVGDGSIPDVAIVDDTRAMATGSDLYLWVRDGLLAVTS
                     DTQSLQRFADAVEHGGASPFYGSSFHTSLAQRYHDGVQWVIGVDMERLELGQDSGPAR
                     QLEALGILDMQHLIGEHEEDGVHAQTRAELTFDQPRRGIAAWLAEPAPMGAMDYVSRN
                     AYFAAGFVMKEPVQVVDEMIDFLGSEDTGFAAGLEEFEREAAIDIRNDIAAAIGGEFA
                     FALESPVLPTPSWKLIVEVYDPVALQNTLEWAVERVNVALAEQFDSAKGLTLEQSDRG
                     RLTYYELRSLDTGISAYYTFVDGYMIASASRALIERSLQFRNSGLSLASSAAFTSLLP
                     VDAEPNFSAVVYQDWGPILGPLTGGLTKAGRAMGPEGQKLFDSLGSLSGPNLTLAYGE
                     ESRITLINTTEGGLLGRGLTSILSLDSLLSVRQLVDRAVQEQASDPSDGAPQGVGRAD
                     RERG"
     gene            3991..4917
                     /locus_tag="VD788_00165"
     CDS             3991..4917
                     /locus_tag="VD788_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV74704.1"
                     /translation="MNPVIELDGLGVRFGRHQILKDLRGDLRGRAIGLLGPNGAGKTT
                     LVHTLLGFHQATEGTARVLGHDIKETAAIRSGIGYMPENDAFIAGMSAVRLVRLMAEL
                     SGLPPREALERAHEALFYVGLGEARYRNVETYSLGMKQMAKLAQAIVHGPRLLFLDEP
                     TNGLDPPHRRRMLDLIREIKQAGEVRIVLSSHLLKDVEEVCDEVLILKQGQVARYCDL
                     EAERQANRKFIEIETTGSNAVFASEIAKLGCDYALDGDKRLKTVLPQGVEIRDLYRVA
                     ADHQIRIRRLDYRRDSLKDIFLRAMESNRGGL"
     gene            4904..5788
                     /locus_tag="VD788_00170"
     CDS             4904..5788
                     /locus_tag="VD788_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74705.1"
                     /translation="MAVYERTYRRYEGELTPSRARITVLPRYAYGEILKSKLFMAFVV
                     VCLTWSFALACVLYIPHNLGLLKIEALGLDPEEVGRFFALWIDPNFFFRFFMWPTFFM
                     ALVVTLVVGPVLVTSDLRNNGLALYFSRPLGRAQYALGKALVLAILLSILTWIPGFLL
                     FLFQSYLEGFDWLRQNYRVGLGIVLGHWIWITVLCLISLAISAYVKWRPVARLTLILL
                     IFVAGGLSALTNFLLGTEWASVINISDMMLVIWANLFGVDPPVSTPAWVAWISPVLFC
                     LVSVWLLSRKLRPYEVVK"
     gene            5785..6747
                     /locus_tag="VD788_00175"
     CDS             5785..6747
                     /locus_tag="VD788_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV74706.1"
                     /translation="MSATERLADSGIVFDNVSKFYGEVLGINRVHLSIPPGITSLVGP
                     NGSGKTTVMNLMTGLLQPSQGEIRVLGIRTDDPEQLFRHVGYCTQYDSFPRGFTGEEF
                     VHAYLRVHGYSAAAAQAKTRKALERVGMTAAAARRVGGYSKGMRQRIKLAQAIAHDPS
                     VLVLDEPLNGLDPMAREEMLDLFRGLAAQGNHVIISSHILHEVDFISDQVILLNEGYV
                     IAEGNIRSVRSEMQKHPLQILIRCDRPAALASRVFEQDGVVEARIDGERGGLLVKTRD
                     ADSFYLAFNRIVLDNDFKIEVVTPADEDVDAVYQYLIGGMKDKA"
     gene            6744..7562
                     /locus_tag="VD788_00180"
     CDS             6744..7562
                     /locus_tag="VD788_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74707.1"
                     /translation="MTTDRLAQLTGVMRLELKKGLLGRRAVPIYLIGLVPLAPVLMFV
                     LVSSLTEIPREFVGLGGATLFFILQFEFILRLIIYFGCVWVFMNLFRGEVIDRSLHYY
                     FLTPIRREILVAGKYLSGWLTATVVFGGATAVCFVIVYAYLGAAGGGDVLGAATILTL
                     LRYLAVVTLACLGYGAVFLVVGLFLRSIIIPAFLIFVWEMANPVLPAALKKVSVLFYL
                     QGLRPLPPPDAPIQIIAEPVSIWIAIPGFLVFTAVTLFAAALRIRRMEIAYGSD"
     gene            complement(7565..8260)
                     /locus_tag="VD788_00185"
     CDS             complement(7565..8260)
                     /locus_tag="VD788_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74708.1"
                     /translation="MTDESRSAWFYVGLGCGGCALLVGVLAIVTVVGGIRFARSVERD
                     LEDPVARTEKAKVLLGTDGLPEGYQAVMSLSVPFVFEMVILADKAAEAGHGLADDTEK
                     LFIYVELIRGDRNWREYASGGGDPSDVLSGQGLRMRGGSEEIGRGTLVSNDSTVDYVS
                     RRGTLNVEDDPFDGIVTFLFVRCEGSKKMRVGVWANRDPAPDARSDELDLRGTCADPQ
                     RIESFLRPLDLCG"
     gene            complement(8257..8844)
                     /locus_tag="VD788_00190"
     CDS             complement(8257..8844)
                     /locus_tag="VD788_00190"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HXV74709.1"
                     /translation="MTAGGLSVVGAQVASGIGLAACAGLRAFLPLLVVGVAGRSGWIP
                     LSEPFAWLESTPALVVFGVAVVLEIVADKVPVVDHALDGLQAWVKPVAGTILAASVLV
                     ELSPLQATVLGLITGGAVAETVHLTKAGLRLASTTTTAGVANPLLSIAEDLGAAAGSL
                     LAIVVPLLVVLVALVTIVIAWRGVRRFSRRRGATR"
     gene            complement(8861..9100)
                     /locus_tag="VD788_00195"
     CDS             complement(8861..9100)
                     /locus_tag="VD788_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74710.1"
                     /translation="MQTVSESQRLIAMLNAIDVGELDGIRAKLASARQACIRLDQVEL
                     ADRLHEAERALFEADLKTYRKRIATVIARLGHLKE"
     gene            complement(9114..9530)
                     /locus_tag="VD788_00200"
     CDS             complement(9114..9530)
                     /locus_tag="VD788_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXV74711.1"
                     /translation="MTAGELMRTEIVSLHEDDAIDEAFDLLVGEHIHGAPVVGADGAL
                     IGVVSQLDIYFGKMTREREAGKRKGKAGLLRVRDVMTAPALSATEDTDVADLCRMMYK
                     LRIHRVPIVRESRVCGIVSSMDVCEAVASGRLVASV"
     gene            9679..>11080
                     /locus_tag="VD788_00205"
     CDS             9679..>11080
                     /locus_tag="VD788_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HXV74712.1"
                     /translation="MLKRIRDLLIGKNRTSAGNPPSTGTVEALAEPPPEPAVRRGPEV
                     VHRPIAREELDPDAVKIIQRLTRFDHGAYLVGGCVRDLLLDRKPKDFDIGTSATPRQI
                     KRLFRNSRIIGRRFRLAHIYFQNGKIIEVATFRAQDDGDAVGDGGNGRGDLLIRDDNQ
                     FGTMEQDALRRDFTINSLFYDVNAETVLDHSDGLGDLRRRTIRTIGDPDVRFKEDPIR
                     ILRAIKFAARLDFDIERETRRALYATRDEIPKAAAPRILEEINRFCRGGAARRSFELM
                     RENGVFEIVLPELAAAYGDDERVWKLLYRFLERFDARIGGGGGEVQTGQILAALLLPA
                     LMSDLGFHFDGSVARPESSDLGAAIDRYRRPLAARLRASRRDQEQCRQLLLTLERLVP
                     RDRLHRNTKRALSRRLALADAMDLLHGLGAVLGGEFSASARAWEGEAGGRDRRSHGDS
                     RGTPAVPSDPAPPARGT"
BASE COUNT         1867 a         3758 c         3625 g         1830 t
ORIGIN      
        1 gtcctcgaac cgagccccac aatccgggca gaacgactcc tcggacgcga cctccgaacc
       61 gcactccccg caggtgaagg tctcgtccgg cggggtggaa tcggcggcgt cgaaaccgag
      121 atgatcgatg acctctttgt agcgatcacg ctcgacgcga agccagtact cctcgtcgac
      181 cgcaacgtgc ccgggcgagc cggtcgattc ccggatcacg atctcgcagc ggacgcggtg
      241 gcgcccgaga cggctcctgg cgtcgcgtgc ttcgtccagt gtgaggaagc cgcagtagtc
      301 gaccatcggc gggtggagcc gggccgcggc cgcctcggac actcgcccgc cgcgtcccgc
      361 cgccggtccc acatccgatc gttcgacgag cgccaccccg caaccggcgc actcggtgaa
      421 cccgggccgg aactcgtcct tgcagaccgg gcaaaacatg cggcgaaggc tagcctggat
      481 ccagtggact gtcaaaaacc ggatacgaac ggtcgccccg agcccaccga ccggtctggc
      541 cgagccgttt cccggggaat cggggtatcc tcgcgatgga gcgagcctcc gttttcccgc
      601 attcccgcgt cgggcggtga ccccggttaa tcaccgggtc gggaggaccg tctttatgag
      661 tgaccaacgc gacggagaga ctcccgtgca acacgcgctc gaagccaaag ccgcgaccga
      721 acccgatctc gaacgcgtct accgggacca ccatcgtcgg gtactccagg cggcctaccg
      781 ggtgaccggc agcgcccagg acgcggagga cgtgctgcaa accgtgttcc tgcggctcgt
      841 ccgacgcgag gggagctcgc cgttgtccga tagtctgggc aactacctgc atcgggcggc
      901 ggtcaacgcg gcgctcgacg tcgttcgctc gcgtaaggcc agtcgttcga ccccgctcga
      961 cagcgtcgaa ccgacgctcg ccgccggatc cgagaccacg ccggacgcga tccagagcga
     1021 ggtcgagatc aagcaaagga tccgaacggc actggcgaga atgagcgcca aggccggtga
     1081 gatattcgtg ctccgctact tcgagggata cggcaaccac gagatcgcga ccatgctcgg
     1141 tacctcgcga agcacggtca acgtgatcct gcaccgtacc agggagaaac ttcgtgggga
     1201 gctcgcttcc taccttggag agacgccatg aaccgcaatg ccgacgactt cgatcgcgag
     1261 ctggatcaag ccctcgaccg catccgtcgg gacgagttgc ccgaggcgac ccgccaggcg
     1321 atctccgagc gcgtgtgggc tcgcgtcggt agcgcgatcg ccgacgccgc gcccgacgag
     1381 tcggagccgc accggatccg cgactgctcg gacttccagg cgctgatacc ggcctacctg
     1441 cgcggcggcc tcaacgaggc gaagtcgctg ctgctcagcg accacgtgac gagctgcgtg
     1501 ccgtgccgaa aaaccctgat ccaagcgcgc gacgcgcggc gccgtgcgcg ggccgcgaca
     1561 gccggcgcga ccgaacccgg ctgggccgcg cgttggagtt ggcggctggc cgccgccgcg
     1621 gtcatcgtcg tcgcgttggt cggcctcagc gtcaagaccg acctgttcac cgtacgcacc
     1681 ggcggcgtga tcttcatcga ggcgatcgac ggcgacgtct tccagatcac cggagacggg
     1741 ccggtgccgc tgcaagtcgg cgactcgctc agcttcgagg acggcaggct gattcgcact
     1801 ggccgtgact ccggcgcgat cttccgaacc tcggacgact cggtcgtcga ggtcagcgag
     1861 cgagccgagc tctccgttca ggaccggcgc aagctgtgga acgtgaggaa ccccgactcg
     1921 gtcatcgagc tgaaccgcgg gagtgtcatc gtcgaggcgt ccgaacaggg ttcgggtcat
     1981 ctgtacgtcg acacgccgga cgcgatgatc agcgtcacgg gtacggtctt cgcggtcaat
     2041 cgcgggatca agggctcgcg ggtgtcggtg ctcgagggtg aggtcgcggt gaatcactcc
     2101 ggcgggacgc acgttctgca cccgggagag caggcgacga cccagcccgg gctcggacag
     2161 gtccccgtcg aggacgagat cgcctggagc cggcagctcg acaagcacct cgcgctcctg
     2221 cgcgagttcg ccgagctcgg ccgagagatc gaccgtgcgg tcgtaccgcc gaacctgcgc
     2281 tatggaaccg cgctcctcga tcgtgtcccc gacgacacct tcgtctacgt cgcgattccg
     2341 aatctcagct cgacgatcag ccaggcctac gacctgatgc gggacaagat ctccggcaac
     2401 gggttgctca accaatggtg ggaagagtcg atcgtcacga gcggcgccga cgcgcagatc
     2461 gagctggcga tggagaagat ccgcagctac ggaccccaga tcggcgaaga gatcgtcgtg
     2521 acggtgggcg gcatcgaccg ggcgcacggc gatccgagcg tgttgatcct ggccgagctc
     2581 gtcgaacccg aggccttcct gcagatggtc cgtaccgacc tgacgggtct gcggttgctc
     2641 gtcggcgacg ggtcgattcc cgacgtcgcc atcgtcgacg acacgcgcgc gatggctacc
     2701 ggcagcgatc tgtacctgtg ggtgagggac gggctgctcg ccgtcacgtc ggacacccag
     2761 tcacttcagc gcttcgccga cgcggtcgag cacggcgggg ccagcccgtt ctacggctcc
     2821 tcgttccaca cgagcctggc ccagcgctac cacgacggcg tgcagtgggt gatcggggtc
     2881 gacatggaac ggctcgaact cggccaggac agcggaccgg cccgccagct cgaggcgttg
     2941 ggcatcctcg acatgcaaca tctgatcggc gagcacgaag aagacggtgt gcacgcccaa
     3001 acccgcgccg agctgacctt cgatcagccg cgccgcggga tcgcggcgtg gctcgccgaa
     3061 cccgccccga tgggtgcgat ggactacgtc tcgcgcaacg cgtacttcgc ggccggcttc
     3121 gtcatgaagg aaccggtgca ggtcgtcgac gagatgatcg acttcctcgg ctcggaggac
     3181 acgggcttcg ccgccggtct cgaagagttc gagcgcgagg ccgcgatcga catccggaac
     3241 gacatcgccg cggcgatcgg cggcgagttc gccttcgcgc tcgagagccc ggtgttgccg
     3301 acgccttcgt ggaagctgat cgtcgaggtc tacgacccgg tcgcgctgca gaacacgctc
     3361 gagtgggccg tcgagcgggt caacgtcgcg ctggccgagc agttcgactc ggcgaagggg
     3421 ctcaccctcg aacagtccga ccgcggccgg ttgacctact acgagctgag atcgctcgac
     3481 accggcatca gcgcgtacta cacgttcgtc gacggttaca tgatcgcgtc ggcctcgcgg
     3541 gcgttgatcg agcggtcgct ccagttccgc aactccggtc tttcgctggc gtcgagcgcg
     3601 gcgttcacgt cgctgttgcc ggtcgacgcc gagccgaact tctcggcggt cgtctaccag
     3661 gactggggac cgatcctcgg cccgttgacc ggtgggctca ccaaggccgg ccgcgcgatg
     3721 ggccccgagg gccagaagct gttcgacagc ttgggctcgc tcagcggtcc caatctgacc
     3781 ctggcctacg gcgaggagag ccgcatcacg ctgatcaaca cgaccgaggg cggtctgctc
     3841 ggccgcgggt tgacctcgat cctcagcctc gacagcctgc tgagtgtgag acaattggtc
     3901 gaccgagcgg tgcaggagca ggcttccgat ccgtctgacg gagcgccgca gggagtgggg
     3961 cgtgccgacc gagaacgcgg ctgagcgacg atgaacccgg tcatcgaact ggacggtctg
     4021 ggcgttcggt tcggtcgcca tcagatcctg aaggatctgc gcggcgacct tcgtgggcgc
     4081 gcgatcggcc tactcggacc gaacggggcg ggcaagacga cgctggtcca cacgctgctc
     4141 ggtttccacc aggcaaccga gggtacggcc cgcgtcctcg gtcacgacat caaggagacc
     4201 gccgcgattc gctcggggat cggctacatg ccagaaaacg acgcgttcat cgccgggatg
     4261 tcggccgtcc ggctggtccg gttgatggcc gagctgagcg ggctgccgcc gcgcgaagcg
     4321 ctggagcgag cgcacgaagc gctgttctac gtcgggctgg gcgaggcgcg ctaccgcaac
     4381 gtagaaacct actccctcgg catgaaacag atggccaagc tggcccaggc gatcgttcac
     4441 ggaccgcggc tgctgttcct cgatgagccg accaacggtc tcgacccgcc gcaccggcgg
     4501 cggatgctcg acctgatccg tgagatcaaa caggccggcg aggtgcgcat cgttctatcg
     4561 tcccacctgc tgaaggacgt cgaggaggtc tgcgacgagg tactgattct caagcagggc
     4621 caggtcgcgc gctactgcga cctcgaggcc gagcgacagg cgaaccgcaa gttcatcgag
     4681 atcgagacga cgggcagcaa cgccgtgttc gccagcgaga tcgcgaagct cggctgtgac
     4741 tacgcgctcg acggcgacaa acggctcaag acggtgctgc cgcagggcgt cgagattcgc
     4801 gacctgtatc gggtggccgc cgaccatcag atccggattc gccggctcga ttaccggcgc
     4861 gattcgctga aggacatctt cctgcgcgcg atggagtcga accgtggcgg tctatgagcg
     4921 gacctatcgc cgatacgagg gcgagctgac gccgagccgc gcgcggatca ccgtcctgcc
     4981 gcggtacgcc tacggcgaga tcctgaagtc caagctgttc atggcgttcg tcgtggtctg
     5041 tttgacctgg tcgttcgcgc tggcctgcgt gctctacatc ccgcacaacc tcgggctgct
     5101 gaaaatcgag gccctcggcc tcgacccgga ggaggtcggc cggttcttcg cgctctggat
     5161 cgaccccaac ttcttcttcc gcttcttcat gtggccgacg ttcttcatgg cgctcgtcgt
     5221 cacgctcgtc gtcgggccgg tactggtgac gtcggatctg aggaacaacg ggctggcgct
     5281 ctacttttcc cgcccgctgg ggcgagcgca gtacgcgctg ggcaaggccc tggtgctcgc
     5341 gatcctgctc tcgatcctca cctggatccc gggattcctg ctcttcctgt tccagagcta
     5401 cctcgaaggg ttcgactggc ttcgacagaa ctaccgggtc ggcctcggca tcgtcctcgg
     5461 acactggatc tggatcacgg tcctgtgcct gatcagtctc gcgatctccg cttacgtcaa
     5521 gtggagaccg gtcgcgcggc tgacactgat cctgctgatc ttcgtcgccg gcggactgtc
     5581 cgcgttgacg aacttcctgt tgggaacgga gtgggccagc gtcatcaaca tctcggacat
     5641 gatgctcgtg atctgggcca acctgttcgg tgtcgacccc ccggtgtcga caccggcatg
     5701 ggtcgcgtgg atctcgccgg tgctcttttg cctggtgtca gtctggctcc tgtcgcgaaa
     5761 actccgcccg tacgaagtgg tcaaatgagc gcgaccgagc gactggccga cagcggcatc
     5821 gtcttcgaca acgtgtcgaa gttctacggc gaggtgctcg gaatcaaccg agtgcacctg
     5881 tcgatcccac cgggcatcac cagcctcgtc ggtccgaacg gttccggcaa gacgaccgtg
     5941 atgaacctga tgaccggcct gctgcagccg agccaaggcg agatccgggt gctcggtatc
     6001 cgcaccgacg acccggaaca gctgttccgc cacgtcggtt actgcacaca gtacgactcg
     6061 ttccctcgcg gcttcaccgg tgaagagttc gtccatgcct acctcagggt gcacggctac
     6121 tccgcggcag cggcccaagc caagacgcgc aaggcgctcg aacgggtcgg catgaccgct
     6181 gcggcggcgc gacgggtcgg cggctacagc aagggaatgc ggcagcggat caagctggcc
     6241 caggcgatcg cccacgatcc gtccgtgctt gtgctcgacg aaccgctcaa cggactcgat
     6301 ccgatggccc gcgaggagat gctcgacttg ttccgtggac tggccgcgca ggggaaccac
     6361 gtcatcatct cgagccacat cctgcacgag gtcgacttca tctcggatca ggtgatcctg
     6421 ctcaacgagg gctacgtgat cgccgagggg aacatccgca gcgtgcgcag cgagatgcag
     6481 aagcacccgc tgcagatcct gatccgctgc gaccgaccgg cggcgctcgc ctcgcgcgtc
     6541 ttcgaacagg acggtgtcgt cgaagcgcgg atcgacggag aacggggcgg actgctggtc
     6601 aagacgcggg acgccgacag cttttatctg gcgttcaacc gcatcgtgct ggacaacgat
     6661 ttcaagatcg aggtcgtcac gccggccgac gaggacgtcg acgcggtcta tcagtatctg
     6721 atcggcggaa tgaaggacaa ggcatgacga ccgaccgcct ggctcaactc accggggtga
     6781 tgcggctcga gctgaagaag ggcctgctcg ggcgccgtgc ggttccgatc tacctgatcg
     6841 gtctcgtccc gctggcgccg gtgttgatgt tcgtgctcgt cagcagcctg accgagatcc
     6901 cgcgtgagtt cgtcggcctc ggcggcgcga cgctgttttt catcctgcag ttcgagttca
     6961 tcctgcgact gatcatctac ttcggctgtg tctgggtctt catgaacctg ttccgcggtg
     7021 aagtgatcga ccgcagcctg cactactact tcttgacgcc gatccgccgc gagatcttgg
     7081 tcgccggcaa gtatctgtcg ggctggttga cggcgacggt cgttttcggc ggtgcgacgg
     7141 cggtctgctt cgtgatcgtc tacgcctatc tcggcgcggc cggcggcggc gacgtgctcg
     7201 gcgcggcgac gatcctcacg ctgttacgct acctcgcggt cgtgacgctg gcctgtctcg
     7261 gatacggagc ggtcttcctg gtggtcggtc tgttcctgcg atcgatcatc atcccggcct
     7321 tcctgatctt cgtctgggag atggccaatc ccgtgctgcc cgcggcgctg aagaaggtca
     7381 gcgtgctgtt ctacctgcag ggcctgcgcc cgcttccgcc acccgatgcc ccgatccaga
     7441 tcatcgcgga accggtgtcg atctggatcg cgattccggg cttcctcgtc ttcaccgccg
     7501 tcaccctgtt cgccgccgcg ctccgcatcc gccgcatgga gatcgcctac ggctcggatt
     7561 aggcctagcc gcagaggtcg agcgggcgca ggaacgactc gatccgctgc gggtcggcgc
     7621 aggtaccgcg gagatcgagc tcgtcggagc gggcgtcggg agccggatca cgattggccc
     7681 agacgccgac ccgcatcttc ttgctcccct cgcaacggac gaacaggaag gtgacgatcc
     7741 cgtcgaacgg gtcgtcttcg acgttcagcg tcccgcgccg actgacgtag tcgaccgtcg
     7801 agtcgttcga gacgagcgtc ccccgaccga tctcttccga cccccctctc atgcgaaggc
     7861 cctgtccgga cagcacgtcg gacgggtcgc caccacccga ggcgtactcg cgccagttgc
     7921 gatcgccgcg gatcagctcg acgtagatga acagcttctc ggtgtcgtcg gccaacccgt
     7981 ggccggcctc cgccgccttg tccgccagga tcaccatctc gaagacgaac gggaccgaca
     8041 gcgacatgac cgcctggtac ccctcgggca acccgtcggt tcccagcagc accttggcct
     8101 tctccgtccg cgccaccggg tcctcgaggt cccgctcgac gctccgggcg aagcggatcc
     8161 cgccaacgac cgtgacgatc gccagcacgc cgaccaacag cgcgcagccg ccgcagccga
     8221 ggccgacgta gaaccaggcc gagcgcgact cgtcggtcat cgggtcgccc cgcgccgtcg
     8281 ggagaaccgt cgtacccccc gccaggcgat cacgatcgtc accagcgcga cgagcacgac
     8341 gagcagcggc accacgatcg ccagcaggct gcccgccgcc gcgccgaggt cttccgcgat
     8401 cgacagcagc gggttggcca cgccggcggt cgtcgtggtg gaggcgaggc gtaggccggc
     8461 cttggtcagg tggacggtct cggcgaccgc accgccggtg atcagcccga gcaccgtcgc
     8521 ctgcaacggc gacagctcga ccagcacgct cgcggcgagg atcgtgccgg cgaccggctt
     8581 gacccacgcc tgcagaccat cgagcgcgtg gtcgacgacc gggaccttgt ccgccacgat
     8641 ctcgagcacg acggccacgc cgaacacgac gagcgccggc gtgctctcga gccaggcaaa
     8701 cggctcggaa agcgggatcc agcccgatcg ccccgcgacg ccgaccacga gcaacgggag
     8761 gaaggcgcgc aacccggcgc aagccgccag cccgatcccg gaggcgacct gcgccccgac
     8821 cacggagagc ccgcctgccg tcaactcgcc gtcgatcgct tcactccttg agatgaccca
     8881 accgcgcgat gaccgttgcg atccgcttgc ggtaggtctt cagatcggcc tcgaacaggg
     8941 cccgctcggc ctcgtgcagc cgatccgcca gctcgacctg atccagccgg atgcacgcct
     9001 ggcgggcgct cgcgagcttg gcgcggatcc cgtcgagctc gccgacgtcg atcgcgttga
     9061 gcatcgcgat caaccgctgc gattccgata cggtctgcat cccggtaccc cactcaaacc
     9121 gacgcgacga ggcgtccgga cgcaaccgcc tcgcagacat ccatcgagct cacgatcccg
     9181 caaacgcggg attcgcgtac gatcggaacg cggtggatcc gcagcttgta catcatgcgg
     9241 cacagatcgg cgacgtcggt gtcctcggtc gcggagagcg ccggggcggt catcacgtct
     9301 cgcacgcgca gcagccccgc cttacccttg cgcttgccgg cttcccgctc gcgggtcatc
     9361 tttccgaagt agatgtcgag ctggctgacc acgccgatca gcgccccgtc ggcgccgacg
     9421 accggcgccc cgtggatgtg ctcaccgacc aggaggtcga acgcctcgtc gatcgcatcg
     9481 tcctcgtgca acgacacgat ctcggttctc atcagctcgc cggccgtcat cgaaatacct
     9541 cccgctaccc cgattcgctc gctattattc gcgtcggttc cgggaatttc cagtccgctc
     9601 aggagtcggg tgcgcgatat ccgtggaatt gtcggaatcg ttgttggtat attggtagcg
     9661 cggccgccat acgagtagat gctgaaacga atcagggatc tgctgatcgg aaagaaccgg
     9721 acctcggccg gcaacccgcc gtcgaccggc acggtcgaag ctctcgccga accgccgccg
     9781 gaaccggcgg ttcgtcgcgg ccccgaggtc gttcaccggc cgatcgcgcg cgaagagctg
     9841 gatcccgatg cggtcaagat catccagcgc ctgacccgtt tcgatcatgg cgcctacctc
     9901 gtcgggggat gcgttcggga cctgttgctc gatcgcaagc cgaaagactt cgacatcggc
     9961 acctccgcca cgccacgcca gatcaagcgg ctgttccgga actcgcggat catcggacgg
    10021 cgtttccggc tggcccacat ctacttccag aacggcaaga tcatcgaagt cgccaccttc
    10081 cgcgcacagg acgacggtga cgcggtcggg gacggtggca acggccgcgg cgatctgttg
    10141 attcgcgacg acaaccagtt cggaacgatg gaacaagacg cactgcgccg cgacttcacc
    10201 atcaactccc tgttctacga cgtcaacgcg gaaacggtgc tcgaccacag cgacggcttg
    10261 ggggatctgc ggcggcggac gatccgcacc atcggcgacc cggacgtgcg cttcaaggag
    10321 gatccgatcc gcatcctgcg ggcgatcaag ttcgccgcgc ggctcgactt cgacatcgag
    10381 cgggaaacgc gacgtgccct gtacgcgacc cgcgacgaga tcccgaaagc cgcggcaccc
    10441 cgcattctcg aagaaatcaa ccgcttctgc cgcggcggtg cggcgcggcg ctcgttcgag
    10501 ctgatgcggg agaacggcgt cttcgagatc gtgctgcccg agctggccgc ggcctacggc
    10561 gacgacgagc gagtctggaa gctgctctac cggttcctcg agcgattcga cgcgcggatc
    10621 ggcggcggcg gcggcgaggt tcaaacgggg cagatcctgg cagcgctgct gttgcccgcg
    10681 ttgatgagcg atctcggttt tcacttcgac ggatcggtcg cccgccccga gtcgtccgat
    10741 ctcggcgccg cgatcgaccg ctaccggcgt ccgctggcgg cgaggctgcg cgcttcgcga
    10801 cgcgatcagg agcagtgccg gcagctgttg ctcacgctcg aacggctggt gccgcgcgat
    10861 cgactccacc gcaacacgaa gcgcgccctt tcccggcgac tcgcgctcgc cgacgcgatg
    10921 gatctgctcc acgggctggg tgcggtgctc ggtggagagt tctcggcgag cgcgcgggcg
    10981 tgggagggcg aggccggcgg acgcgaccgg cggtcgcacg gcgactcgcg tggcacaccc
    11041 gcggtcccga gcgatccggc gccgcctgcg cgagggaccg
//
LOCUS       DASPGN010000006         3351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1288941, whole genome shotgun sequence.
ACCESSION   DASPGN010000006 DASPGN010000000
VERSION     DASPGN010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3351
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1288941"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VD788_00210"
     CDS             complement(<1..651)
                     /locus_tag="VD788_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXV74713.1"
                     /translation="MIEYDPETIEPRWQAEWQRTQAFRVGSASPGGTRFYCLEMLPYP
                     SGKLHMGHVRNYSIGDAIARYQRMLGRQVMHVIGWDAFGMPAENAAIQHGENPASWTR
                     RNIEIMRGQLKRLGTSYDWEREIATCDPRFYRWNQWFFLKMLERGQAYRARRRLNWCD
                     HCATVLANEQVVDGRCWRCDGPVRHREFEQWFLRISDYAQELLDGLQDLDDWPAKVR"
     gene            complement(648..1073)
                     /gene="nusB"
                     /locus_tag="VD788_00215"
     CDS             complement(648..1073)
                     /gene="nusB"
                     /locus_tag="VD788_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXV74714.1"
                     /translation="MGRRRQAREHALQMLFQIDVTGAPPDEVFREFWSDQPVDAGTRE
                     FSESLVLGVITDRPALDEWIRGSTAHWRIERMAVVDRNVLRMAGHELRQRPAVPAAVI
                     IDEAIEVAKKFGGEDSGAFVNGILDTIRRRIEAESGADR"
     gene            complement(1083..1550)
                     /gene="ribH"
                     /locus_tag="VD788_00220"
     CDS             complement(1083..1550)
                     /gene="ribH"
                     /locus_tag="VD788_00220"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXV74715.1"
                     /translation="MARTLEGVLDARGIRVALVVSRFNDAITNRLLIGAEDCLERHGG
                     TAEHRTIVRVPGSWEIPQAARKLATAGQHDAIIALGALIRGETPHFDLLAAEVAKGLA
                     QVAADSGVPVTFGVLTTDTVDQAMDRSGAKFGNKGWDAALSAIEMVNLFRRMA"
     gene            complement(1639..2520)
                     /gene="mtnP"
                     /locus_tag="VD788_00225"
     CDS             complement(1639..2520)
                     /gene="mtnP"
                     /locus_tag="VD788_00225"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HXV74716.1"
                     /translation="MSSAEPTRIAIIGGSGLYELAGLDEVGERVIETPFGAHSPGLFV
                     GRLEDRPVAFLARHGRAHRLLPAEINYRANIFALKTLGVERIVSASAVGSMRTGIRPQ
                     DVVLVDQFIDRGQHRQATFFGDGVVAHISFADPVCPELRAALTGIAGRLGARAHDGGT
                     YLCIEGPAFSTRAESRLYRSWGVDVIGMTNMHEAKLAREAEICYATMALVTDYDCWHD
                     GEDDVSVAALLDNLRANGELAAGVVRQAVRSMPVERSACGCSTALEHAILTPPGSIPH
                     GTLRRLEPILGKYVRVP"
     gene            2707..>3351
                     /locus_tag="VD788_00230"
     CDS             2707..>3351
                     /locus_tag="VD788_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74717.1"
                     /translation="MSRSFRWNGSSSGGVVLSVTALVAISAVALAVDGQLARSGPPAV
                     SLAVGAVICARLADRLAAEGAIRLALAFVLASLVALPPMASVAVAGLAGVAGRGRGVG
                     LSGGDRPRALVSLVVGVATTEVLGLGLGGRLPREAWGGLPWLVVGFVVLHLVDRATPG
                     AEPQRRLARRSRWLLASCNLPLAWFLVRIHFEFHWAEALIVVAVVTGFQAALARL"
BASE COUNT          499 a         1172 c         1109 g          571 t
ORIGIN      
        1 ccgcaccttg gccggccaat cgtcgaggtc ctgcaggccg tcgagcagct cctgcgcgta
       61 atccgagatg cggaggaacc actgctcgaa ctcgcgatgg cggacaggcc cgtcacagcg
      121 ccagcagcgg ccgtcgacga cctgctcgtt ggccagcacg gtcgcgcagt ggtcgcacca
      181 gttcaatcgc cgccgggcgc gataggcctg tccgcgttcc agcatcttga ggaagaacca
      241 ctggttccat cggtagaacc gcggatcgca ggtcgcgatc tcgcgctccc agtcgtagct
      301 ggtgccgagt cgcttgagct ggccgcgcat gatctcgatg ttccggcgag tccagctcgc
      361 cgggttctcg ccgtgctgga tcgccgcgtt ctcggcgggc atgccgaacg cgtcccagcc
      421 gatcacgtgc atcacctgac ggccgagcat ccgctggtag cgggcgatcg cgtcgccgat
      481 cgagtagttt cgcacgtgtc ccatgtgcag cttgccgctc ggatacggca gcatctcgag
      541 gcagtagaac cgcgtcccgc cgggcgacgc cgaccccacg cgaaaggcct gcgtacgctg
      601 ccactccgcc tgccaccgcg gctcgatcgt ctcggggtcg tattcgatca tcggtccgca
      661 cccgattcgg cctcgatccg ccggcgaatc gtgtcgagaa tcccgttgac gaaagcgccg
      721 ctgtcctcgc cgccgaactt cttcgccact tcgattgcct cgtcgatgat caccgccgcc
      781 ggcaccgccg gtcgctgcct cagctcgtgg ccggccatcc gcagcacgtt gcggtcgacg
      841 accgccatcc gctcgatccg ccagtgcgcg gtcgatccgc ggatccactc gtcgagcgcc
      901 ggtcggtcgg tgatcacacc gagcaccaac gactcggaaa actcgcgggt cccggcatcg
      961 accggctggt cgctccagaa ctcgcggaac acctcgtccg gcggtgcgcc cgtgacgtcg
     1021 atctggaaca gcatctgcag cgcgtgctca cgcgcctgcc ggcgacgacc caacgtccct
     1081 tcctacgcca tccggcggaa caggttgacc atctcgatcg cggacagcgc cgcgtcccaa
     1141 cccttgttgc cgaacttggc gcccgatcga tccatcgcct gatcgaccgt gtccgtcgtc
     1201 agcaccccga aggtgaccgg gactccggag tcggccgcga cctgggccag acctttcgcg
     1261 acctcggccg ccagcagatc gaagtgcggt gtctccccgc ggatcagcgc gccgagcgcg
     1321 atgatggcgt cgtgctggcc agcggtcgcc agcttgcgcg cggcctgcgg gatctcccac
     1381 gagccgggca cgcggacgat cgtccggtgt tcggccgtcc cgccgtggcg ctcgaggcag
     1441 tcctccgccc cgatcagcag gcggttcgtg atcgcatcgt tgaaccggga gacgacgagc
     1501 gcgacccgga tgccccgcgc atcgagaacg ccttcgagcg tgcgtgccat cggtgtgccc
     1561 tcaaaccgct ccgggatccg gtaccgaaag tccttcaaaa agaataattt tgcagtatag
     1621 gaagcgtcgg ggacgaggtc aaggaacgcg aacgtacttg ccgaggatcg gctcgaggcg
     1681 ccgcagcgtc ccgtgcggga tcgacccagg cggcgtgagg atcgcgtgct cgagcgcggt
     1741 cgagcaaccg catgccgagc gttcgaccgg catcgatcga accgcctggc gcacgacccc
     1801 cgccgccagc tcgccgttgg cacgcaggtt gtcgagcagc gccgcgaccg aaacgtcgtc
     1861 ctcgccgtcg tgccagcagt cgtagtcggt cacgagcgcc atcgtcgcgt aacagatctc
     1921 ggcctcccgc gccaacttgg cctcgtgcat attggtcatt ccgatcacgt cgacgcccca
     1981 ggagcgatag agccgggact cggcccgggt cgagaaggcc ggcccttcga tgcagaggta
     2041 ggtgccgccg tcgtgggcgc gcgcgccgag ccggccggcg atgcccgtga gcgccgcccg
     2101 gagctcgggg cagaccggat ccgcgaacga gatgtgcgcg accacgccgt cgccgaaaaa
     2161 ggtggcctga cgatgctggc cgcgatcgat gaactggtcg acgaggacga cgtcttgcgg
     2221 ccggatcccg gtgcgcatcg atccgaccgc cgacgcggag acgatgcgct cgacacccaa
     2281 cgtcttgagc gcgaagatgt tggcgcgata gttgatctcg gcgggcagca atcggtgtgc
     2341 cctaccgtga cgcgcgagga acgcgacggg ccgatcctcg aggcgtccga cgaacaggcc
     2401 cggtgaatgc gctccgaacg gcgtttcgat cacccgctcg ccgacctcgt cgagcccggc
     2461 gagctcgtag agaccgctcc cgccgatgat cgcgatcctc gtgggttcgg cggaggacac
     2521 ggcatacctc gttctgaatt acaattgtta aaaagacgtg tgggacgacc atccacgcat
     2581 cgtcgttccc ggcacgattc tacgtgatcc actcccgatt ggcactggca caaactcgaa
     2641 tcgtacgtac cgtatactcg tcctgttgtt ataagcatgt ttctatcagg cataggcgca
     2701 tatgtagtgt cgaggtcgtt tcgctggaac ggttcgtcgt ccgggggtgt cgtcctgtcg
     2761 gtcaccgccc tggtggcgat ctccgccgtc gcgctcgcgg tcgacggcca gcttgctcgg
     2821 tccgggccgc cggccgtcag cctcgccgtc ggcgcggtga tctgcgcgcg gttggccgac
     2881 cgactcgcgg cggagggggc gatccgcctg gcgctcgcgt tcgttctcgc atccctcgtc
     2941 gcgttgccgc cgatggcgtc ggtggcggtc gcgggactcg ccggcgtcgc cggtcggggt
     3001 cgcggagtcg ggctctcggg cggggaccgc ccgcgggcgc tcgtctcgct ggtcgtcggg
     3061 gtcgccacca ccgaggtgct cgggctcggg ctcggcggcc ggctgcccag ggaagcctgg
     3121 ggcggtctgc cgtggctcgt ggtcggcttc gtcgtgctac acctcgtcga ccgcgcgaca
     3181 cccggcgccg aaccgcagcg ccgcctggcg agacggtcgc ggtggctgct cgcctcatgc
     3241 aacctcccgt tggcctggtt cctcgttcgg atccacttcg agttccactg ggccgaggcg
     3301 ctgatcgtgg tcgccgtcgt cacgggcttc caggcggcgc tcgcccggct c
//
LOCUS       DASPGN010000007         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_997, whole genome shotgun sequence.
ACCESSION   DASPGN010000007 DASPGN010000000
VERSION     DASPGN010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..971)
                     /gene="meaB"
                     /locus_tag="VD788_00235"
     CDS             complement(42..971)
                     /gene="meaB"
                     /locus_tag="VD788_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HXV74718.1"
                     /translation="MPTKEALLAGDVRALARAISLVEDQQPAAVELLRAIFPHTGAAT
                     VIGLTGAPGSGKSSLVDRLAARYRATDHKVGIIAVDPSSAFSGGAILGDRIRMQSHTA
                     DPSVFIRSMATRGHLGGLARATNDAIDLMDAAGFDPVIVETVGVGQDEVDVVKTADVV
                     AVVLVPGMGDDIQAIKAGILEIADLFVINKADHKGADRLQADLAYMLELTDRETAKPE
                     ILRTVAIRNEGIDRLHDGIASFMSNTGRSRRRERRRDRAEARFRAVLVERLLRRVVER
                     ALPADELGRLIDAIAARELDPYTAAERVTRSLE"
     gene            complement(978..2123)
                     /locus_tag="VD788_00240"
     CDS             complement(978..2123)
                     /locus_tag="VD788_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXV74719.1"
                     /translation="MDFELSDEQQMLRETVREFANAEVAPHVMEYDEKQEFPRELILK
                     AGELGLLGIIFPEEYGGAGLGYVEYALVVEELSRVDGSVGISVAAHNGLCANHIYLAG
                     TERQKQDFLTPLASGTRIGGWSLTEPTAGSDASGTKTTAREDGDHWILNGAKTFTTHG
                     SVGDVIVVFGVTDADRGNRGISAFIVERGHPGFRPGKKENKMGLRASDTAEVVMEDCR
                     VPADQLLGERGQGFIDAMRVLDGGRISIAALALGMARGAYEAALKYSKEREQFGRPIA
                     EFQAIRFMLAEMATKIDAAALLTYRAAWMKDRGRKVTKEAAMAKLFASEIGVEVADKA
                     LQVFGGYGYVKDFPVEKYYRDMKLCTIGEGTSEIQRLVISREILKSL"
     gene            complement(2212..>2489)
                     /locus_tag="VD788_00245"
     CDS             complement(2212..>2489)
                     /locus_tag="VD788_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74720.1"
                     /translation="LVEDHVRARRLADALAEHPDFRIDPAGVQTNIVVAGLREPASTD
                     RVLRRLAQAGVLAGAMGPGRIRFVTHLDVDDRGLSRAIDVVARLRSD"
BASE COUNT          391 a          898 c          790 g          410 t
ORIGIN      
        1 gccgaccgcg attccgatgt ggtcgatctt tcgcgccatc gtcactccag cgaccgcgtc
       61 acgcgctcgg ccgcggtata ggggtcgagc tcgcgcgcgg cgatcgcgtc gatcagacgc
      121 ccgagctcgt cggccggcag cgcgcgctcg acgacccgtc gcagcaggcg ctcgaccagc
      181 accgcgcgaa aacgggcttc ggcccgatcg cgtcgccgct cgcgacggcg cgatcggccg
      241 gtgttcgaca tgaacgaggc gatgccgtcg tgcagtcggt cgatcccttc gttgcggatc
      301 gcgaccgtcc gcaggatctc gggcttcgcg gtctcgcggt cggtcagctc caacatgtag
      361 gccagatcgg cctgcagccg gtcggccccc ttgtggtccg ccttgttgat gacgaacagg
      421 tcggcgatct cgaggatgcc ggccttgatc gcctggatgt cgtcgcccat tcccgggacg
      481 agcacgaccg cgacgacgtc ggccgtcttg accacgtcga cctcgtcctg gccgacgccg
      541 acggtctcga cgatcaccgg gtcgaagccg gccgcatcca tcagatcgat cgcgtcgttg
      601 gtcgcccgcg ccaacccgcc gagatggccg cgcgtcgcca tcgagcgaat gaaaacgctc
      661 ggatcggccg tgtggctctg catccggata cgatcgccca agatcgcgcc tcccgagaac
      721 gcgctcgacg gatcgacggc gatgatgccg accttgtggt ccgtcgcgcg atagcgcgcc
      781 gccagccgat cgaccagcga cgacttgccc gagccgggcg ccccggtcag accgatcacc
      841 gtcgcggcac cggtgtgcgg gaagatcgcc cgcagcagtt cgaccgccgc cggctgctgg
      901 tcttcgacca gactgatcgc acgcgccaac gcgcgaacgt ccccggctag cagtgcttcc
      961 ttggtcggca tcgacgctca gaggctcttg aggatctccc gggaaatcac caagcgctgg
     1021 atctcgctcg ttccctcgcc gatcgtgcac agcttcatgt cgcgatagta cttctcgacc
     1081 ggaaagtcct tgacgtagcc gtatccgccg aagacctgca gcgccttgtc ggccacctcg
     1141 acgccgatct cggaggcgaa cagcttggcc atcgccgctt ccttggtgac cttccgcccg
     1201 cggtccttca tccaggcggc gcggtaggtc agcagcgccg cggcatcgat cttggtggcc
     1261 atctccgcca acatgaaccg aatcgcctgg aactcggcga tcggccgacc gaactgctcc
     1321 cgttccttcg agtacttgag cgcggcttca tacgccccgc gggccatccc gagcgcgagc
     1381 gccgcgatcg agatccggcc accgtcgagc acgcgcatcg cgtcgatgaa accctgcccg
     1441 cgctccccga gcaactgatc ggcgggaacg cggcagtcct ccatcaccac ctcggcggtg
     1501 tccgaggcgc ggagccccat cttgttctcc ttcttgccgg ggcgaaaacc gggatgcccg
     1561 cgctcgacta tgaacgccga gatgccgcgg ttgccgcgat cggcgtcggt gacgccgaag
     1621 acgacgatca cgtcaccgac cgagccgtgc gtggtgaacg tcttcgcccc gttgaggatc
     1681 cagtggtcgc cgtcctcgcg cgcggtggtc ttggtgccgc tggcgtccga gcccgcggtc
     1741 ggctcggtca gactccaccc gccgatccgc gtgccgctgg cgagcggggt gaggaagtcc
     1801 tgcttctgtc gctcggtccc ggcgaggtag atgtggtttg cgcaaagccc gttgtgcgcg
     1861 gcgaccgaga tgccgaccga gccgtcgacg cgggacagct cctcgaccac caatgcgtac
     1921 tcgacgtagc ccaagccggc gccgccgtac tcctccggga agatgatgcc gagcaggccg
     1981 agctcgcccg ccttcaggat cagctcgcgc gggaattcct gcttctcgtc gtactccatc
     2041 acgtgcggcg cgacctcggc gttcgcgaac tctcgcacgg tctctcgtag catctgctgt
     2101 tcgtcgctga gctcgaagtc cacggttgtt tctccggaaa gcgaagggtc atcggcgaaa
     2161 atgaggacga ttatgacaca acgccgggaa cgacgtcgcg cctgcgcggc gtcagtcgct
     2221 gcgcagccgc gccacgacgt cgatcgcccg cgacagcccc cgatcgtcga cgtcgagatg
     2281 cgtgacgaaa cggatccggc ccggccccat cgcgccggcc aacacacccg cctgcgccag
     2341 ccgtcgcagc acgcggtcgg tcgacgccgg ctcgcgcaac cccgcgacaa cgatgttcgt
     2401 ctgcacgccg gccggatcga tgcgaaagtc gggatgctcg gccaacgcgt cggccagacg
     2461 ccgagcccga acgtgatcct cgaccagcc
//
LOCUS       DASPGN010000008         9463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_469458, whole genome shotgun sequence.
ACCESSION   DASPGN010000008 DASPGN010000000
VERSION     DASPGN010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469458"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VD788_00250"
     CDS             <1..931
                     /locus_tag="VD788_00250"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXV74721.1"
                     /translation="PASDEFLIIGHESLGVVEQVGPAVDELRPGDLVVATVRRPGHSL
                     YDQIGLQDMTTDDVYYERGINLRHGFLAEYYAESADYLVRVPRACRKVGVLLEPLSVA
                     EKGIAQAYEIQRRLRVWRPHRAAVMGAGTLGLLATMVLRLRGLDVTVFGLATPPYLNG
                     ELAQELGARYVSTRQTSIADGAQRYGPFDLVFEATGNSGVVFESASALAKNGVLVLTS
                     ITGGARRLEIPADRINLEFVLGNKVMVGSVNAHREHFELGAGDLATCNAEYPGWLDKL
                     LTHPVRGLANYGQMLQLLTDGRDVIKVFVDVAH"
     gene            970..1212
                     /locus_tag="VD788_00255"
     CDS             970..1212
                     /locus_tag="VD788_00255"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV74722.1"
                     /translation="MRCSEFVDFLDRYVERRLDASESEEFDRHLRVCPDCVRFLENYR
                     QTIELVRVASRDDDRIPAEVPRELIAAVRAAIRRRE"
     gene            1209..2294
                     /locus_tag="VD788_00260"
     CDS             1209..2294
                     /locus_tag="VD788_00260"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXV74723.1"
                     /translation="MSGRPVRVEVRYPLRDGRMLLRTSLDWDRDLPPVAVDRAGGRYR
                     FELELERDAFVYFKPLIDTGSEPRWSRGEDYLLLSAGDRPLVIHPFFTDDAGCSVCEL
                     AERRSEESGRSHGFRVFLPPGYHENTAERLPVIYMQDGQNLFFGEEAFAGRHWRVAET
                     LDLLTSMNLVQRAIVVGVHPAARETDYTSPGYEAYGRYLVDELVPWIDANYRTCRRPE
                     QTAVIGSSLGGVVSLYLAWQWPRVFGLAACMSSTFGWADDLMRRISVERRRRLRIYLD
                     SGWPRDNYEVNRLMRELLLHRGYVLGRDLLYLAFPQAPHDETAWSTRLHLPLQFLFGP
                     AAAPPSLRSGLRASEKNRTVLFPASSS"
     gene            complement(2230..7746)
                     /locus_tag="VD788_00265"
     CDS             complement(2230..7746)
                     /locus_tag="VD788_00265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXV74724.1"
                     /translation="MFSGLELREELYRTAAQVVRRARGSQAQASWLVRLPLRQPPDPD
                     DLIELEAEYRRLAGLAVAQIPRPVELIRRDGLAALLLEDIGGRPLADVLRLGPLPLDS
                     SLAIAVDLATALGALHGRNLTHRFLDPTVVVWAANAGRSQIIDLGQEGLTRGRGTPIE
                     SQPAIALRYISPEQSGRMNRDLDYRSDFYSLGVILYQLLAGRVPFPFDDPLELIHCHI
                     ARAPTALGDLDRAVPPALEVVVGKLLEKNAEQRYQSARGIIADLTLCLEQWRASGRID
                     PFAPGRHDVPERFSVVQKLYGRESELDRLIRAFERTAAGRPGVMLVCGPPGVGKTALI
                     RELYKPITATRGYLLTGKVDQLARTVPYAPIVEAIRSLVQLLLTEDDARLDRIRTALD
                     NELGPTVGVLCEVVPELELIVGARPASPAVTPLEAQNRLTLVFQRFLSVIADPTRPLA
                     VFLDDLQWIDDATLRLLPDLLGSPLIRGLFVIGAYRDNEVGAEHPLLATVAELRRRGC
                     AVESLRLGALRIGELARLVGDTLQRPADACGSLVETIESKTAGNPFFAIQLLRSLHQR
                     GVIVVDPDHAWWSYDESALASLPISDNVVELMAQRIGDMPVETRELLSLGACIGASFD
                     LPTLAVVSRRPVDRIVDRLRPALDAGLILAAGTPAGRFRFLHDRVQLAAYGLIPEDRR
                     APTHVTIGRLLLAGSGGRRSGGRLFDIVSHLNIGRRLLDDPAERVELARLNLDVGRAA
                     KASAAFAESRSYLLSADELLDAAHWEWDYPLAFEVHRELAEAEYLCGDFGAAEALADE
                     LMRRAADDLDRARVHELRVALFENMSRPPQAIAAGRDGLALLDSRIPETDEAKRETLA
                     SELDRIRFGLQGRAIAALVELPEVRDERIRLVMQFLTRLWAPVYVSGDLVLCNLLSAR
                     LVSLSLEHGNSSQSAYGYVTHAITVGPGHGNYGAAYEWGLLALAVNERFGDRQLRAKI
                     QQQFGAHVNLWRAPFASCLDHAREAYRAGVEVGDLAYASYGILVESWYAFLTGPDLEQ
                     FERRTRESVGTLEGLHMAGIAELEQAIAHWARALRGATFDPTRLSSPDFDAGAFASGH
                     AATPFFMAILELMELQLAYTFGQYERAERVAASVEPRLAAVEGTVWIPLHDLYCGLAL
                     ARMAGSDPAADPQRLDRAQGLARRLGRLAEHCPPNFRAPALLVEAELARVTGRDGQAL
                     RCYEEAIDAASRGGVLMFQALAHELYAGYWIDRGNPRIAGIYLRDAHEQYRRWGAVAK
                     LRELERRHGELLAGPDGSRLGGTGRTLPIDLASTIKSARALTADILTDDYFERLVRIV
                     IENAGAGRGLLLCEQDGTLTVKAEATLAERPVEPSGVDSAAAERRLPSSIAQYVWKTG
                     KVVLLRNAAEDHRFVGDPYIAAHNPRSVLCLPLVHQGRRCGLLYLENNVATDAFGPER
                     LAVMEVLAAQAAVSLEVARLYGEMKAEVSHRRSVEQSLRHAVDELERLKRRLQADNVY
                     LQEQIQQANSFDTIIGGSPELRRCLNQVELVAPTDATVLILGETGTGKELVARAIHDR
                     GPRRERPLVKVDCSSISAGLVESELFGHVRGAFTGAHDHRTGRFQLADGGTIFLDEIG
                     ELAPETQVKLLRVLQEREFEPVGSNKTVRVDVRVIAATSRDLPSEIAAGRFRQDLFYR
                     LNVFPVDLPPLRARRRDIPELVTHFLARAGRRYGKPVSGISEQALDLLVGYDWPGNVR
                     ELQNVVERATVVCRGDRLEPESILLPGRAAAAATAPVTVAPVPSAGHDHRAASLEELE
                     RTHITSVLRRTGGVIEGPRGAARILDLSPSTTRFKMKKLGIRRSDFSPTPEGRS"
     gene            8010..8651
                     /locus_tag="VD788_00270"
     CDS             8010..8651
                     /locus_tag="VD788_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV74725.1"
                     /translation="MHQARSSAAALDSADDPRTDEVLIAEIARGDETAFEALVQRHAG
                     QLLAVARRMLRDAEEARDAVQEALLSVYRHAATFERHCRATTWLHRIVVNAALMRLRG
                     RKRRRELSIEDLLPKFAEDGHHAHPIAAWPADVTEELEREQRATIVRRCIDRLPEAYR
                     TVLLLREIDGLNAAETARLLRTSANAVNIRLHRARLALRTLIDPILRGETVVG"
     gene            8736..9128
                     /locus_tag="VD788_00275"
     CDS             8736..9128
                     /locus_tag="VD788_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74726.1"
                     /translation="MNAISKLTLVLILILAWVGPTRAEHTSTRFAGAKVNSGTVSHAV
                     IDGADVLTLSDDFVVPDAPAPHWQVVDSRGNVYLLQRLVVKGDVEHRSINLPAQIDDV
                     ARVQIWCAWAEALLGEAVFEPAIERSRR"
     gene            9125..>9463
                     /locus_tag="VD788_00280"
     CDS             9125..>9463
                     /locus_tag="VD788_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HXV74727.1"
                     /translation="MNGDGRRHLPEYLIEAAGLGLFMLSAATFATLLESAQSPVRAAI
                     GDPWLRRLLMGTAMGLTLVLIVYSPWGRRSGAHINPAVTLTFYRLGKVSSRDATHYIV
                     AQVVGGVAGLM"
BASE COUNT         1469 a         3287 c         3244 g         1463 t
ORIGIN      
        1 ccccgcgagc gacgagtttc tgatcatcgg tcacgagtcg ctcggcgtcg tcgagcaagt
       61 cggaccggcg gtggacgagc tccgtcccgg cgacctcgtc gtcgccaccg tgcgccgtcc
      121 gggtcactcg ttgtacgacc agatcgggtt gcaggacatg acgaccgacg acgtctacta
      181 cgagcgcggc atcaacctgc gtcacgggtt cctcgccgag tactacgcgg agagcgccga
      241 ttatctggtc cgggtgccgc gcgcctgccg caaggtcggt gtgctgttgg aaccgctcag
      301 cgtggcggag aaggggatcg ctcaggccta cgagatccag cgtcggctgc gcgtctggcg
      361 cccgcaccgg gccgcggtga tgggggccgg cacgctcggc ttgctggcga cgatggtgct
      421 ccgcctgcgc ggtctggacg tgaccgtctt cgggctggcg accccgccgt acctcaacgg
      481 cgagctggca caggagctgg gagcgcgcta cgtcagtacg cgccagacct cgatcgccga
      541 tggggcacaa cgctacggac cgttcgatct ggtcttcgag gcgaccggga actccggcgt
      601 ggtgttcgaa tcggcctccg cgttggccaa gaacggggtg ctggtgctga ccagcatcac
      661 cggcggcgcg cgacggctcg agataccggc cgatcgcatc aacctggagt tcgtcttggg
      721 caacaaggtc atggtcgggt cggtcaacgc ccaccgcgag cacttcgagc tcggagccgg
      781 cgacctggcc acgtgcaacg cggaataccc gggatggttg gacaagctgt tgacccaccc
      841 ggttcgcgga ctcgccaact acggccagat gctgcagctg ttgaccgacg gccgcgacgt
      901 gatcaaggtg ttcgtcgacg tcgcccattg accagaccac ccgcctgccg acgtgagctg
      961 gagcgcgtga tgcgatgctc ggaattcgtc gactttctcg accgctacgt cgagcgccgg
     1021 ctcgacgcga gcgagagcga ggagttcgat cgccacctcc gcgtctgtcc cgactgcgtg
     1081 aggttcctcg agaactaccg gcagacgatc gagctcgtgc gggtcgcatc cagggacgac
     1141 gaccgaatcc cggcggaggt cccgcgggag ctgatcgcgg cggtccgcgc cgcgatacgg
     1201 cggcgcgaat gagtggccgt ccggtgcggg tcgaggttcg gtacccgtta cgcgacgggc
     1261 gtatgctgtt gcgcacttcg ctcgattggg accgggacct gccgccggtc gcggtcgacc
     1321 gagccggtgg ccgctatcgc ttcgagctcg agctcgagcg cgacgcgttc gtctacttca
     1381 agccgttgat cgacaccgga agcgagccgc gttggtcccg cggtgaagac tacctgctgc
     1441 tttccgccgg cgatcgcccg ctcgtgatcc acccgttctt caccgacgac gccggctgca
     1501 gcgtgtgtga gctcgccgag cgtcgatcgg aggagtcggg acgctcccac ggcttccgcg
     1561 tattcctgcc acccggctac cacgagaaca cggccgagcg cctgcccgtg atctacatgc
     1621 aggacgggca gaatctgttc ttcggcgaag aagccttcgc cggccggcac tggcgcgtcg
     1681 ccgagaccct cgacctcttg acctcgatga acctggtcca gcgtgcgatc gtggtcggcg
     1741 tccatccggc ggcccgcgag accgactaca cctcaccggg gtacgaagcc tacggtcgat
     1801 acctggtcga cgagctggtg ccgtggatcg acgcgaacta ccgcacctgc cgccgccccg
     1861 agcagacggc ggtgatcggc tcctcgctcg gtggcgtggt gtcgctgtac ctcgcctggc
     1921 agtggccgcg cgtcttcggc ttggccgcct gcatgtcgag caccttcggc tgggccgacg
     1981 acctgatgcg gcggatctcg gtcgagcgcc ggcgcaggct gcgcatctac ctcgacagcg
     2041 gctggccgcg ggacaactac gaggtcaatc ggctgatgcg agagctgctg ctgcaccgcg
     2101 gctacgtcct cggacgcgac ctgctctacc tggcctttcc gcaggcgcct catgacgaga
     2161 cggcgtggtc gacacgcctg catctgccac tgcaattcct gttcggcccg gcagcagcgc
     2221 cgccatcgct caggagcggc cttcgggcgt cggagaaaaa tcggaccgtc ttattcccag
     2281 cttcttcatc ttgaaccggg tcgtgctcgg cgagagatcg aggattcgcg cggctccgcg
     2341 cggcccttcg atcacaccgc ccgtccgccg cagcacgctc gtgatgtggg tccgctcgag
     2401 ctcctcgagc gaggccgcgc ggtgatcgtg gcccgcgctc ggcaccggcg cgaccgtcac
     2461 gggtgccgtg gccgctgcgg ccgcgcgccc gggtaacagg attgattccg gctccagtcg
     2521 gtcgccgcgg cagacgaccg tggcgcgctc gacgacgttt tgcagttcgc ggacgttgcc
     2581 cggccaatcg tagccgacga gcaggtcgag cgcctgctcg ctgatgccgc tgaccggttt
     2641 gccgtaccgc cggccggcgc gcgccaggaa atgggtgacc aactcgggga tgtcccgccg
     2701 ccgcgcccgt aacggcggca ggtcgaccgg gaagacgttg agccggtaga acaggtcctg
     2761 acgaaagcga cccgcggcga tctcgctcgg caggtcgcgg ctggtggcgg cgatcacgcg
     2821 gacgtcgacg cggaccgtct tgttgctccc gaccggctcg aactcgcgtt cttgcagcac
     2881 gcgaagcagc ttgacctgcg tttccggcgc gagctcgccg atctcgtcga ggaagatcgt
     2941 tccgccgtcg gccagctgga accgcccggt gcggtggtcg tgcgccccgg tgaacgcgcc
     3001 tctcacgtgg ccgaacagct cgctctcgac gagacccgcc gagatcgagc tgcagtcgac
     3061 cttgaccaat ggccgctcgc gtcgtggtcc gcgatcgtgg atcgcccgcg ccacgagctc
     3121 cttgccggtg ccggtctctc cgaggatcag caccgtcgca tcggtcggcg cgaccagttc
     3181 gacctggttg aggcagcgtc gcagctccgg gctgccgccg atgatcgtgt cgaaggagtt
     3241 cgcctgctgg atctgctcct ggagatagac gttgtccgcc tgcagccggc gcttgagacg
     3301 ctccagctcg tcgacggcgt gtcgcagcga ctgctcgacc gaccgccggt ggctcacttc
     3361 ggccttcatc tcgccgtaca accgggcgac ctcgagcgaa accgcggcct gcgcggccag
     3421 cacctccatc accgccagcc gctcggggcc gaaggcgtcg gtggccacgt tgttctcgag
     3481 gtacagcagg ccgcagcggc ggccctgatg gaccagcggt aggcacagca cggagcgcgg
     3541 gttgtgggcg gcgatgtacg gatcgccgac gaaacggtga tcctcggccg cgttccgcag
     3601 caggacgacc ttgcccgtct tccacacgta ctgcgcgatc gacgacggca ggcgtcgttc
     3661 ggccgccgcg gagtcgaccc cgctcggctc gaccggtcgc tcggccaggg tcgcctcggc
     3721 cttgacggtc agcgttccgt cttgctcgca caacaacagg ccgcgaccgg ccccggcgtt
     3781 ctcgatgacg atgcgcacga gacgttcgaa atagtcgtcg gtgaggatgt cggcggtcag
     3841 cgcccgcgcc gacttgatcg tcgaggcgag gtcgatcggc aacgtgcgac ccgtgccgcc
     3901 gagacgactt ccgtccggcc ccgcgagcag ttccccgtgc cgtcgctcca gctcgcggag
     3961 cttggccacc gccccccaac ggcgatattg ctcgtgtgcg tcgcgcaggt agataccggc
     4021 gattcgcggg ttgccgcggt cgatccagta acccgcgtac aactcgtggg ccagcgcctg
     4081 gaacatgagg actccgccgc gcgaggccgc atcgatcgcc tcctcgtagc agcgcagcgc
     4141 ctgtccgtcc cgaccggtca cccgcgccag ctccgcctcg accaacaggg ccggggcgcg
     4201 aaaattcggc gggcagtgct cggcgagccg gcccagccgc cgggccagac cttgcgcacg
     4261 gtcgaggcgt tgcggatcgg ccgccgggtc cgatccggcc atcctcgcca gcgccagacc
     4321 gcagtacagg tcgtgcagcg gaatccagac cgttccctct acggcggcca accgcggctc
     4381 gacgctcgcc gccacacgct cggcacgctc gtattggccg aaggtgtagg cgagctgaag
     4441 ttccatcagc tccaggatcg ccatgaagaa cggcgtcgcc gcgtggccgg aggcgaaggc
     4501 gcccgcgtcg aaatccggac tgctcagtct cgtcgggtcg aaggtcgcgc ctctcagcgc
     4561 acgcgcccag tgagcgatcg cctgctcgag ctcggcgata cccgccatgt gcagaccctc
     4621 gagcgtgccg accgactcgc gcgtccggcg ctcgaactgc tcgaggtccg gaccggtgag
     4681 gaacgcatac cagctctcga cgaggatccc gtaggaggcg taggccaggt cgccgacctc
     4741 gaccccggcg cggtaggcct cgcgcgcgtg atcgagacag ctcgcgaacg gcgcgcgcca
     4801 caggttgacg tgtgccccga actgctgctg gatcttggcg cgcagctggc gatcgccgaa
     4861 ccgctcgttg acggcgagcg cgagcaaacc ccactcgtac gccgcgccgt agtttccgtg
     4921 cccgggcccg accgtgatcg cgtgcgtcac gtaaccatag gccgactgcg acgagttgcc
     4981 gtgttccagc gagagcgaga cgagtcgcgc ggacagcagg ttgcacaaca ccaagtcacc
     5041 cgagacgtaa accggtgccc acagccgagt caggaactgc atcaccaacc ggatccgctc
     5101 gtcgcggacc tccggcagct cgacgagcgc tgcgatcgcg cgaccctgca ggccgaaccg
     5161 gatacgatcg agctccgacg ccaacgtctc gcgtttggcc tcgtcagtct cggggatccg
     5221 gctgtcgagc aacgcgagcc cgtctcgacc ggcagcgatc gcctgcggcg gacgcgacat
     5281 gttctcgaac agcgcgaccc gcagctcgtg gacacgtgct cgatcgaggt cgtcggccgc
     5341 ccgtcgcatc agctcgtcgg cgagggcttc ggccgcgccg aagtcgccgc acaggtactc
     5401 ggcctcggcc agctcgcgat ggacttcgaa ggccagcgga tagtcccact cccagtgtgc
     5461 ggcgtccaac agctcgtccg cactcagcag atacgacctc gattcggcga acgccgccga
     5521 cgccttggcc gcccgtccca cgtcgaggtt caaccgcgcg agctcgacgc gttcggccgg
     5581 gtcgtcgagc agccgccggc cgatgttgag atggctgacg atgtcgaaca gccgaccgcc
     5641 ggagcgccgg ccgccgctac ctgcgagcag cagccgaccg atcgtcacgt gcgtcggcgc
     5701 gcgccgatcc tcggggatca gcccgtaggc cgccagctgc acacggtcgt gcaggaagcg
     5761 gaaccgtccg gcaggcgtgc cggcggccag gatcaaccct gcgtcgagcg ccggccgcag
     5821 ccgatcgacg atccgatcga ccggacgccg gctgacgacc gccagcgtcg gcaggtcgaa
     5881 gctcgctccg atgcaggcgc ccaacgagag cagttcgcgc gtctcgaccg gcatgtcgcc
     5941 gatgcgctgg gccatcagct cgacgacatt gtccgagatc ggcaggctgg ccaacgcgga
     6001 ctcgtcgtag ctccaccacg catgatccgg atcgacgacg atcacgcccc gctggtgcag
     6061 gctgcgtagg agctggatcg cgaagaacgg gttcccggcg gtcttggact cgatcgtctc
     6121 gaccaacgac ccgcacgcgt ccgccgggcg ctgcagcgtg tcgccgacca atcgcgccag
     6181 ctcgccgatg cgcaaagccc cgagacggag cgactcgacg gcgcagccgc gccgccgcag
     6241 ctcggcgaca gtcgccagca gcgggtgttc ggcgccgacc tcgttgtccc gataggcacc
     6301 gatgacgaac agcccgcgga tcaacgggct ccccagcagg tccggcagca ggcgcagcgt
     6361 ggcatcgtcg atccattgca ggtcgtcgag gaagacggcc agcggccgcg tcggatcggc
     6421 gatcaccgac aggaatcgct gaaagacgag cgtcaagcgg ttctgtgcct cgagcggtgt
     6481 gacggccggc gaggccggcc gggccccgac gatcagctcg agctcgggta cgacctcgca
     6541 cagcacgccg acggtcgggc ccagttcgtt gtcgagcgcg gtccggatgc ggtcgagtcg
     6601 cgcgtcgtct tcggtcagca acagctgaac gagcgatcgg atcgcttcga cgatcggcgc
     6661 gtacggaacg gtgcgcgcca gttgatcgac cttgccggtc agcagatagc cgcgcgtcgc
     6721 ggtgatcggt ttgtacagct cgcggatcag cgcggtcttg ccgacgccgg gcggcccgca
     6781 gaccagcatc acgcccgggc ggcccgccgc cgtccgctcg aacgcgcgaa tcaagcgatc
     6841 gagctccgac tcgcgtccgt agagcttctg caccacgctg aaccgttccg gtacgtcgtg
     6901 gcgccccgga gcgaacgggt cgattcgacc actcgcccgc cactgctcga ggcagagggt
     6961 gaggtcggcg atgatcccac gcgcgctttg ataacgctgc tcggcgttct tctcgagcag
     7021 cttgccgacc accacctcca gcgccggagg cacggcccgg tcgagatcgc ccagcgcggt
     7081 cggtgcccgc gcaatgtggc agtggatcag ctcgagcggg tcgtcgaagg ggaacggcac
     7141 gcggccggcg agcaactggt agaggatcac gccgagcgag tagaaatcgc tgcggtagtc
     7201 gagatcgcgg ttcatccgac ccgactgttc gggggagatg tagcgcagcg cgatcgccgg
     7261 ttgactttcg atgggcgtgc cgcgaccgcg ggtcagcccc tcctgaccga ggtcgatgat
     7321 ctgactccgg cccgcattcg ccgcccagac cacgaccgtc gggtcgagga agcggtgggt
     7381 caggttccgg ccgtgaagcg cgccgagcgc ggtggccaga tcgacggcga tcgccagcga
     7441 gctgtcgagc gggagcggtc cgagccgaag cacgtcggcc aacgggcggc cgccgatgtc
     7501 ctcgagcagc agcgccgcga gaccgtcgcg ccggatcagc tcgaccggcc gcgggatctg
     7561 ggcgacggcg agtccggcga gccggcggta ctccgcctcg agctcgatca ggtcgtcggg
     7621 atcgggcggc tggcgcagcg gcaggcggac gagccagctg gcttgcgcct gcgagccgcg
     7681 ggcgcggcgc accacctgcg cagcggtgcg gtacagttcc tcgcgcagct ccagaccaga
     7741 aaacacgccg gcctcacccg ggccacgccc ccttgcggtc gattggccgg attatacagc
     7801 cggcggccgc aacatccggt ggcacggccg caagatgctg cgggcgcccg gttccccacc
     7861 gcgggctcgg acacggaatc gggccgaacc gtgcgggtct ccctcttgtt cggaccgggc
     7921 acgcggcttg ctgtcccgaa cgggcaccat ccgccccgtg gctggcgaat ctcgcagccg
     7981 gcggggtccg gcggtcggag gtgggggcga tgcaccaagc aagaagctcc gcagcggcac
     8041 tcgattcggc cgacgacccg cggacggacg aagtgttgat cgccgagatc gctcgcggcg
     8101 acgagaccgc tttcgaagcc ctggtccagc gtcatgccgg ccagctcttg gccgtcgcac
     8161 gccggatgct ccgcgacgcc gaggaggcgc gcgacgcggt acaggaagcg ttgctgtccg
     8221 tctaccgtca tgccgctacg ttcgagcgtc attgccgcgc gacgacctgg ctccaccgca
     8281 tcgtcgtcaa cgccgcgttg atgcggctgc gcggccggaa gcgacggcgc gagctgagca
     8341 tcgaagacct gttgccgaag ttcgccgagg atggccatca cgcccacccg atcgccgcct
     8401 ggcccgccga cgtcaccgag gagctcgagc gcgagcagcg cgcgacgatc gtccggcgct
     8461 gtatcgacag gctgccggag gcgtaccgca cggtgctgct gctgcgggag atcgacggtc
     8521 tgaacgccgc ggagaccgcc cggttgctgc gcaccagcgc caacgccgtc aacatccgcc
     8581 tccaccgcgc acggctcgcg ctgcgcacgt tgatcgaccc gatcctccgc ggcgagaccg
     8641 tggtcggcta gcttcgcgaa agagcgcacg gcgccacggc atccaatccg acgtcgctgc
     8701 ggcgcgcacg tcgcccgccg acggaggatc aagcgatgaa tgcgatctcg aagctgaccc
     8761 tggtgctgat cctgattctg gcgtgggtcg gcccgacccg cgccgagcac acgtcgacga
     8821 ggttcgccgg ggccaaggtg aactcgggaa ccgtctcgca cgcggtcatc gacggtgccg
     8881 acgtgttgac gttgtccgac gattttgtcg tacccgacgc accggcgccc cactggcagg
     8941 tcgtcgactc gcgcgggaac gtctatctgc tgcaacgtct ggtggtcaag ggcgacgtcg
     9001 agcatcgctc gatcaacctc ccggcccaga tcgacgacgt cgcgcgcgtg cagatctggt
     9061 gcgcttgggc cgaggcgctg ctgggcgagg cggtgttcga gccggcgatc gagcgatcgc
     9121 ggcgatgaac ggcgacggga gacggcatct gcccgagtac ctgatcgagg cggcggggtt
     9181 ggggttgttc atgctctccg ccgccacgtt cgcgacgctg ctcgaatccg cgcaatcgcc
     9241 ggttcgtgcg gcgatcggcg acccgtggct gcgccgtctg ctgatgggaa cggcgatggg
     9301 actgaccctg gtgctgatcg tctactcgcc gtggggccgc cgttccggcg cgcacatcaa
     9361 cccggcggtc acgttgacct tctaccgctt gggcaaggtg tcgtcgcgag acgcgacgca
     9421 ttacatcgtg gcgcaggtcg taggcggcgt ggccggactg atg
//
LOCUS       DASPGN010000009         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_586716, whole genome shotgun sequence.
ACCESSION   DASPGN010000009 DASPGN010000000
VERSION     DASPGN010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586716"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..743
                     /locus_tag="VD788_00285"
     CDS             153..743
                     /locus_tag="VD788_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV74728.1"
                     /translation="MRFTGSTLGPSVPRRGNLLTRQAGRAVLSALGWRLEGRWPDLRR
                     MVVVLAPHTSNWDFFLAICVVVALGVRAHWIGKHTLFRWPFGRLMRYLGGTPIVRDAS
                     IGRVAQIVEQIKRSDEFILGVAPEGTRRRAPRWKSGFYHVAVGAGVPILLAYLDYSQR
                     VVGLGPVFHPTGDERSDLASIRSFYDKRWARFPDQV"
     gene            complement(759..>2104)
                     /locus_tag="VD788_00290"
     CDS             complement(759..>2104)
                     /locus_tag="VD788_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74729.1"
                     /translation="ARLAPDAPSRRELTADPRERAYLEAIELLYAEGDKKERDLAYAD
                     AMRALSERYPDDIEARAFYALAILGTAQGVRDFRVYMHAGAVAEEVFAASPRHPGAAH
                     YLIHSYDDPVHAPLGLRAARVYAQIAPAASHAQHMISHIYLALGHWEESVDANVKSFD
                     VSVSRRERRQLGVDSLNYHSLHWLEYSFLQLGRFDDAWRTLTAMRDYATESGSARALW
                     HHAAMRAAWIVETGGRRATDEIRTDATQVTGAAADLFASGYAAVLAGQPERAVAAVGR
                     MDARFDSAAAGHLCGQTDGYEDTSQRDLVAVEVMRTSLRALIELDRGDTDAAVALLDQ
                     ATATEDAMELDFGPPVIVKPSHELYGEVLLRLDRPAEARVQFDRSLERAPRRSRSLAG
                     LAIAAARIGDGESLASACDELKAVYAGADAAVTLPEACGGAATAAPAAFTRAVAR"
BASE COUNT          310 a          722 c          749 g          323 t
ORIGIN      
        1 gctcatcgcg ctccccgtct ggtacacggc gatcgaggac ttggcggcga cgctggccgg
       61 gatgctgccg gtcggcgcga ccgcgcggct ggcccgccgg cgcgccgttc tgtccgactg
      121 aacgccccga agctgtgtca caatcgagcc ggttgaggtt caccggctca acactcgggc
      181 catcggtacc gcgccgaggc aacctgctga cccgccaggc gggacgcgcc gtgctgtcgg
      241 ctctcggttg gcggctcgag ggacgttggc cggacctgcg gcggatggtc gtggtgctgg
      301 cgccgcatac atcgaactgg gatttcttcc tggcgatctg tgtcgtcgtc gctcttggcg
      361 tgcgggcgca ttggatcggc aagcacacgc tgttccgctg gccgttcggg cggttgatgc
      421 gctatctcgg cggcacgccg atcgtccgcg acgcgtcgat cggccgcgtg gcacagatcg
      481 tcgagcagat caaacggagc gacgagttca ttctcggtgt cgcgcccgaa ggcacacgac
      541 gacgtgcccc acgctggaag tcgggcttct atcacgtcgc ggtcggcgcg ggggtaccga
      601 ttctgttggc ctacctcgac tactcgcagc gcgtggtcgg actcggaccg gtgtttcacc
      661 caaccggcga cgagcggagc gatctggctt cgatccggtc gttctacgac aagcggtggg
      721 cccggttccc ggaccaggtg tgaagcggcg cgcaggcgtc atcgggcaac ggcacgggtg
      781 aacgcggccg gtgccgccgt cgcggcgccg ccgcacgcct cgggcagcgt caccgccgca
      841 tcggcgccgg cgtagaccgc cttcaactcg tcgcaggccg acgcgagcga ctcaccgtcg
      901 ccgatgcgag cggcggcgat cgccaggccg gcgagcgagc gcgacctgcg cggtgcacgt
      961 tcgagcgatc tgtcgaactg gacgcgggct tccgccggac ggtcgagccg cagcaacacc
     1021 tcgccgtaca gctcgtgcga cggcttgacg atcaccggcg gaccgaagtc gagctccatc
     1081 gcgtcctcgg tcgcggtcgc ctgatcgagc aacgcgaccg cggcgtcggt gtcgccccga
     1141 tcgagctcga tcagcgcgcg caggctggtc cgcatcacct cgacggcgac gaggtcgcgc
     1201 tgcgacgtgt cctcgtaacc gtccgtctgg ccgcagaggt gccccgcggc ggcactgtca
     1261 aaacgcgcgt ccatccggcc gacggcagcg accgcccgtt ccggctggcc ggccagcacg
     1321 gcggcgtagc ccgaggcaaa cagatcggcg gccgctccgg tcacctgcgt cgcatcggtc
     1381 cggatctcgt cggtcgcccg acggccgccg gtctcgacga tccacgccgc gcgcatcgcc
     1441 gcgtgatgcc acagcgcacg cgcagagccg ctctcggtcg cgtagtcgcg catcgccgtc
     1501 aacgtgcgcc aggcgtcgtc gaagcggccc agctgcagga aggagtattc gagccagtgc
     1561 agcgaatggt agttgagcga gtcgacgccg agctgcctcc gctcgcggcg cgagacggag
     1621 acgtcgaacg acttgacgtt ggcgtcgacc gactcctccc agtggccgag cgccaagtag
     1681 atgtgcgaga tcatgtgctg ggcatgcgag gcggccggcg cgatctgagc gtagacccgt
     1741 gccgcacgca agccgagcgg tgcgtgaacc ggatcgtcgt aggaatggat caggtagtgc
     1801 gccgcgccgg gatgacgcgg gctcgcggcg aacacctcct cggcgaccgc gccggcatgc
     1861 atgtagacgc gaaagtcacg cacgccctgg gccgtgccga ggatcgcgag cgcgtagaac
     1921 gcgcgggcct cgatgtcgtc gggatagcgt tcggacaatg cccgcatcgc gtcggcgtag
     1981 gccaggtcgc gttccttctt gtcgccctcg gcgtagagca gctcgatcgc ctcgaggtac
     2041 gcgcgctcgc gtggatcggc cgtcagctcg cgccgcgacg gcgcatcggg agcgagacgc
     2101 gcaa
//
LOCUS       DASPGN010000010         7909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_704124, whole genome shotgun sequence.
ACCESSION   DASPGN010000010 DASPGN010000000
VERSION     DASPGN010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704124"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..506)
                     /locus_tag="VD788_00295"
     CDS             complement(114..506)
                     /locus_tag="VD788_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74730.1"
                     /translation="MLARYKIYRGARPADDPAPHGTRQDTREHTRHPLRPDAEQVARF
                     LDDPDDPHWRIFRRAYLALLEDRFAADRAPFDALAREATEGDVFIGCNCPTRNNPDVG
                     RCHTVLALGFMRAKYPRLRVRCPDDRRR"
     gene            complement(506..1732)
                     /locus_tag="VD788_00300"
     CDS             complement(506..1732)
                     /locus_tag="VD788_00300"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HXV74731.1"
                     /translation="MTAVRVSNARVRRALLWINGLSPGPEPDVLPLPGPRVGSDWTRR
                     MVARLGFVQVDPISAVERAQHHILFTRNPHYRQPDLDRALEIDRVLFENWTHDAAILP
                     VEYFPYWRHYMRRFRRFEIHAGYRRYFAPVTDAGAKRVLARLRREGPLLPRDLGDERA
                     NHVDPSFPAPTLAKITLEWLWRTGRVAVCRREGRQKVYDLTERVIPARYHEERVGRDA
                     YVDWACRESLTRLGAGSPAQIARLFDAVSKEQAAAWCRRRLGREVVELRVDGADGSAG
                     GPSYAVERSVAMWSSLPAPDSELRLLNPFDPLIHDRKRTLRLFGFDYSVEIWVPPNKR
                     RYGYYVMPILEGERFTGRVDAKTDREAGLLRVLGLWWEPGVHPTRKRMAALEGELTAL
                     ARFAGAGRVEFARGIR"
     gene            complement(1756..3570)
                     /locus_tag="VD788_00305"
     CDS             complement(1756..3570)
                     /locus_tag="VD788_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74732.1"
                     /translation="MILYWSRGAAAARRFDRCTRLQQRPPAVTVVARGVRWPPVDGSP
                     GWAARALLGGAGPACLAIGLLAAVRSSWVTDDAFISFRYAENLVGGLGLVFNAGERVE
                     GYTNFLWTMWCAAGLGLGIDPVPWALASGVACYGASIVLLVVHHLRLRAALGSTGLTL
                     PVAAVVAALHRDWAVWATGGLETAAFTLLALIGFLLLVGRDGPTTGCALAAGAVLALA
                     AMTRPDGVIFAAVAGPWLLFFAGSTARSRWREAAAFVVGFAVLFLPFLSWRLAYYGEL
                     VPNTYHAKSAALAWYGQGWHYLRLFFTKYWVLALAAPLAAVSALRLRAKPVWFAQAAL
                     AAACALAYTFWVWRVGGGFMFARLLVPTLPFWAILLDLALLPLASRRAWQPAATSAVA
                     LALLFTPQPIEGRRIVHGIADEPRHYARDAVAEVDRRAAILRRYFAGLDVTVGFTGSE
                     ARLVYRAAVPRAIECEAGLTDPVIARRPLERRGRPGHEKHATLSYLVDVRRTHFVLNR
                     HAWRIIGAENRVPPVPVEMDGVRAWLLHWDPRLVAELRGRGARVPDFPAALDRIIAGL
                     DRLEPLAVEALYRDLCRFYFDHVDDPARRQAFTRHLRS"
     gene            complement(5249..7126)
                     /locus_tag="VD788_00310"
     CDS             complement(5249..7126)
                     /locus_tag="VD788_00310"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV74733.1"
                     /translation="MASATLALGLSQALAYLWNEDLWWYLASGRRILAERWVPDRDTF
                     LLATGEAAGWVTHSWLWTVVVAAVDELAGLRGLSLLSGLLVAVLVVLVFARGPLDRFG
                     LLDGALALVVLSAGNQRLLLRTEVAGWLLAALFVLLLERDRKPTSRTLLAMLAIQWLW
                     ANLHGGFYLGILIVLAYAAGGWLSGDPRRPAWWWPPAFAAVSLITPSLWHGELGYAFM
                     VMSRHGYLLEWRPTFEQLALREPWIWLLCATSGAASLIRLRSPRLGSRALLLGGFGVI
                     GLFALRFVPAFAVVWAVVVVLNLHDRGWRGSAPRRPPWRVAHGFAAATFCLLCLAAAA
                     ELWRLRPAFELGQSRDTWASLAPGSTAAAAADFIERERLPAPIFNDMELGGYLIHRLH
                     PGYRLFIDTRQLDLRRVERYIEMVTREAAWAEAQREFGFRTVVLSNPATVSPLALRAF
                     LLRDPEWELVFLDAQAAVFVREPPAALPRVLDVPPFLPADSRPGRWGTVVARVLLRQD
                     PWALAAHLLAVLGDLGRLDELERHATRLLETRPADPELLRLRGAALLFGGDPRRAVDD
                     LARSSALRPGHVETRYLLARALADDGRPDQASVEVDRVLAAEPDHRGALRLRRTLAVP
                     P"
     gene            complement(7182..>7909)
                     /locus_tag="VD788_00315"
     CDS             complement(7182..>7909)
                     /locus_tag="VD788_00315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HXV74734.1"
                     /translation="TRVEHRPSYALAIVDLESGESVKAETGAMVSMSGNVRVESGMQG
                     GLFKSALRKVLGGESFFVNTFTADGGPGEVTFAPSLPGDVDEVRLNGELYVQSGSYLA
                     GGPTIDIDIKWGGARSFFAAEGLFLLHLRGQGEVMLSSFGAIHRVELDGARSLMVDTG
                     HVVAFEPSVSWDVRKVSGLFTSLISGEGLVVEFSGRGKVWLQTRSNQAFLDWLLPRLP
                     TSRTSSGGRGDVGGILGNILRGS"
BASE COUNT         1237 a         2852 c         2785 g         1035 t
ORIGIN      
        1 gctatcgcgt gcggatgagc aacgaagtgc agctcgacgt cggcggcgga ggaccggggt
       61 tccacgtcaa gacgttcaac ggcgacgtct ggatccgcaa agcgaactga ccgtcagcgt
      121 cgcctgtcgt caggacagcg cacccgcagg cgtgggtact tggcccgcat gaacccgagc
      181 gccagcaccg tgtgacagcg gccgacgtcg gggttgttcc gcgtcgggca gttgcagccg
      241 atgaagacgt caccctcggt cgcttcgcgc gccagcgcgt cgaacggcgc gcggtcggcg
      301 gcgaatcgat cctcgagcag cgccaggtac gcgcgccgga agatgcgcca gtgcggatcg
      361 tccgggtcgt cgagaaagcg cgccacctgc tcggcgtcgg gtcgcagcgg gtgtcgcgtg
      421 tgctcgcgcg tgtcctgtcg cgtcccgtgc ggcgccggat cgtccgccgg ccgtgcgccg
      481 cggtagatct tgtaccgagc gagcatcaac gtatcccgcg tgcgaactcg acccgcccgg
      541 cgccggcgaa ccgcgcgagc gcggtcagtt cgccctcgag cgccgccatc cgctttcgcg
      601 tcgggtgcac gccgggttcc caccacagac cgaggacccg cagcaggccc gcctcgcggt
      661 cggttttggc gtcgacgcgc ccggtgaagc gctcgccttc gaggatcggc atcacatagt
      721 aaccgtaccg gcgtttgttc ggcggcaccc agatctcgac cgaatagtcg aagccgaaca
      781 gccgcagggt gcgcttgcga tcgtggatca acggatcgaa cgggttcaac agacgcagct
      841 cgctgtcggg tgccggcagc gacgaccaca tggcgaccga ccgttcgacg gcgtacgacg
      901 ggccgccggc cgagccgtcc gcgccgtcga cgcgcagttc gacgacctcg cgcccgagac
      961 ggcgacgaca ccaggccgcc gcctgctctt tggagaccgc atcgaacagg cgcgcgatct
     1021 gcgcggggct gccggcaccg agacgtgtca gactctcgcg gcaggcccag tcgacgtagg
     1081 cgtcacgacc gacccgttcc tcgtggtaac gcgccgggat cactcgctcg gtcaggtcgt
     1141 agaccttctg gcgcccctcg cgacggcaga ccgcgacccg gccggtgcgc cacagccact
     1201 cgagcgtgat cttggccagc gtcggtgccg ggaagctcgg gtcgacgtgg ttggcccgct
     1261 cgtcccccag atcgcgcggg agcagcggtc cttcccgccg cagccgggcc agcacgcgct
     1321 tcgcaccggc gtcggtgacc ggcgcgaagt agcgacgata gccggcatgg atctcgaaac
     1381 ggcgaaagcg gcgcatgtaa tgccgccagt agggaaagta ctcgaccggc aggatcgccg
     1441 cgtcgtgcgt ccagttctcg aacagcaccc ggtcgatctc gagcgcccgg tcgagatcgg
     1501 gttggcggta gtgtggattg cgcgtgaaca ggatgtggtg ctgggcccgc tcgacggccg
     1561 agatcgggtc gacctggacg aagccgagcc gcgctaccat ccgccgcgtc cagtccgatc
     1621 cgacgcgggg accgggcagc ggcaagacgt ccggctccgg cccgggtgac aggccgttga
     1681 tccagagcag cgcgcgacga acgcgggcgt tggagacccg gacggcggtc atcacgcgat
     1741 tctacccgcc gcgcgtcagc tccgcagatg gcgcgtgaac gcctgccggc gcgccgggtc
     1801 gtcgacgtgg tcgaagtaga agcggcacag gtcgcggtac agcgcctcga cggcaagcgg
     1861 ctcgagccgg tcgagcccgg cgatgatccg atcgagcgct gccgggaagt cgggcacccg
     1921 cgcaccgcgg ccgcggagct cggcgacgag ccgcggatcc cagtgcaaca gccaggcgcg
     1981 gacaccgtcc atctcgaccg ggaccggcgg gacccggttt tcggcgccga tgatccgcca
     2041 cgcgtggcgg ttgagcacga agtgggtgcg ccgcacgtcg accaggtagg agagcgtggc
     2101 atgcttctcg tgacccggcc ggccgcgtcg ttccagcgga cggcgtgcga tgaccgggtc
     2161 ggtcagaccg gcctcgcact cgatcgctcg cggaaccgcc gcgcggtaga ccagtcgtgc
     2221 ctcggagccg gtgaacccga cggtcacgtc gaggccggcg aagtagcgtc gcaggatcgc
     2281 cgcgcggcgg tcgacctcgg ccaccgcgtc gcgggcgtag tggcgcggct cgtcggcgat
     2341 cccgtggacg atgcgtcgtc cctcgatcgg ctgcggcgtg aacagcaggg cgagcgcgac
     2401 ggccgaggtc gcggccggtt gccaggcgcg acgggaggcg agtggcaaga gcgcgagatc
     2461 gagcaggatc gcccagaacg gcaacgtcgg cacgagcaat cgcgcgaaca tgaacccgcc
     2521 gccgacgcgc cagacccaga acgtgtaagc caaagcgcaa gccgcggcga gcgccgcttg
     2581 cgcgaaccag accggtttcg cgcgcaggcg gagcgccgag accgcggcga gcggcgcggc
     2641 gagtgccagc acccagtact tggtgaagaa cagacgcagg tagtgccagc cctgtccgta
     2701 ccaggccagc gccgcggatt tggcgtggta ggtgttcggc accagctcgc cgtagtaggc
     2761 gagccgccac gacaagaacg gcaggaacag cacggcgaaa cccacgacga acgccgccgc
     2821 ctcacgccag cgggagcgcg ccgtcgatcc cgcgaagaac aggagccacg gccccgccac
     2881 tgcggcgaag atcacgccgt cggggcgcgt catggcggcc agcgccagta ccgcgccggc
     2941 cgcgagcgcg caacctgtcg tcggcccgtc ccgaccgacg agcagcagga agccgatcag
     3001 cgcgagcagc gtgaacgccg ccgtctcgag cccgccggtg gcccagaccg cccagtcacg
     3061 atggagggcg gcgacgaccg cggcgacggg cagcgtgagc ccggtcgagc cgagggccgc
     3121 gcgcagcctc aggtgatgga ccacgagcag cacgatcgac gcgccgtagc aagcgacgcc
     3181 cgacgccagc gcccacggaa ccggatcgat gccgagaccg agcccggccg cgcaccacat
     3241 cgtccagagg aagttcgtgt agccctcgac gcgctcgccg gcgttgaaca ccaagccgag
     3301 cccgccgacg agattctcgg cgtagcgaaa cgagatgaac gcgtcgtcgg tgacccaact
     3361 cgaccggacc gcggcgagca ggccgatcgc gaggcacgcc ggccccgctc ccccgagcag
     3421 tgctcgggcc gcccaacccg ggcttccgtc gaccggcggc caacgaacgc cgcgcgcgac
     3481 gaccgtcacg gccggcggcc gctgttgcag acgggtacaa cgatcgaaac gccgtgcggc
     3541 cgcggctcca cgcgaccagt ataggatcat cgggcgccgg tggtcgacgt gttacccttc
     3601 gcggtcggac ggctgcaggg ccgcttttgg gggtaacgct gaccggcaac gagaagccac
     3661 gacgccggcc cgtcggtccc gccgtctggc tctggatcgt cgcggtcgtc atcgcgacct
     3721 gcctgcccta tctgcggctc gcggccggcg ccgagcgcta cgccgcggcc ggtgaccgct
     3781 acgattggct gctcccgccg tacgccgagg acggtctggc ctataccgca tgggtcaagc
     3841 aggcggcgga cggcgcgtgg ctgttcgagg tgaagttcac ccagctcccg catcgcgcag
     3901 tcctgttcca accggtcttt ctcgtcgcgg gctggctcgc cgcgctcacc ggctcgagcg
     3961 ccggcttcgc gctgttcctc gcgcgcgtcg tcgcggtgac gctgttcctg ctggcgctcc
     4021 accagttcgc cgcggtcttt ctcggcgacg ggagcgagcg cgccgtcgcg ctcgcgctcg
     4081 tcagcctgtc ctccgggttc ggcggctggc tgccgcccgg cgcggcgccg tcggccgacc
     4141 tgtggatgcc ggagatcaac acgttgtgga cgttggtctg gaacccggtg ttcgtcgtcg
     4201 ggctgacgct gatgctgctc gcgatcggtc cggtggcgcg ggcgaccgcc tgccggccgc
     4261 agcccaaccg acgaccgttc ttgctcgccg gcgcgtcgtg cggcctgctc gcgttcgtcc
     4321 acggctacga cgtgccgctg gtcgtgctcg tcgtgacggc ggtgacggtc gttcgtctcg
     4381 gccgcgccgc cacctggccg ctggcatcgt tcttctccct ggcgctgccg ccggcgttgc
     4441 tgcagtggct gctcgccgct cagcacccgg tgctgtcggc acacggtctg aagggcgcga
     4501 tgacgagccc gccgccggcc gtctacctgc tggccttcgg tctaccgggg ctgctcgccg
     4561 cctacggtgc gatccgcgtg tggcgcgcgg gcgtgctcga ccgtttcgcc ccgctcgtga
     4621 tctgggtcgt cgcctcgttc gcgctggcct acggtccgtt ctggttccaa cgcaagttga
     4681 tcatggggat ccacctcccg ctgtgcctgc tggcggccgt cggtctgtcc gacgcggcgc
     4741 gtcgtctggc cgcgggccgt cgcgggctgt tcgtcccggt cgtcggcgcg gtcgtgctcg
     4801 cctccgccgc gacccatctc cgcgtggcgc ggtcgatcga ggccgagctg cgtcacgaca
     4861 cgagcgcata cttcgtcccg gcctcgctcg accgcgcgtt gcgctttctc gacgagcgga
     4921 ccgaccccgc ttcgacggtt ctcgcgcact accgggcggc ccgtcacatc cccgggacga
     4981 gcggcaacac ggtgacgctc ggtcactggg cccagtcggt cgatctcgaa agccacatgc
     5041 gttggctggg acgtctgttc gcaccggccg cgggcgacgg caacgacaaa cggcggatgc
     5101 tgcgcgggtc gccgatcgac tacgtgctgg tcgagcccgc gatggtcgag cagtggctga
     5161 acggccgcgt cccacgctgg ctcgaggcgg tcgggccgac cgtctacgac gcgggtgacg
     5221 tgcgcgtcat cgaggtctcg cgatcggctc acggcgggac ggcgagcgtc cgccgtagcc
     5281 gcagcgcccc gcgatggtcg ggctcggcgg ccagcacgcg gtcgacctcg accgacgcct
     5341 ggtcgggacg gccgtcgtcg gccaacgccc tggcgagcag gtaacgcgtc tcgacgtggc
     5401 cgggccgcag cgcgctgctg cgggccaggt cgtcgaccgc ccgccgcggg tcgccgccga
     5461 acagcagcgc cgcgccgcgc agccgcagca gctccgggtc cgccggtcga gtctcgagca
     5521 atcgcgtggc gtggcgttcg agctcgtcga gacgaccgag gtcgccgagc acggcgagca
     5581 ggtgtgccgc cagggcccac gggtcctgcc gcagcaagac ccgtgcgacg accgtgcccc
     5641 agcggccggg ccggctgtcg gccgggagga acggcggcac gtcgagcacc cgcggcagcg
     5701 cggccggtgg ttcgcgtacg aagaccgccg cctgcgcgtc gagaaacacg agctcccact
     5761 ccgggtcgcg cagcaggaac gcgcgcagcg ccagcgggct gacggtcgcg ggattcgaca
     5821 gcacgacggt gcggaagccg aactcgcgct gcgcctcggc ccaggccgct tcccgggtca
     5881 ccatctcgat gtagcgctcg acccgccgca ggtcgagctg ccgcgtatcg atgaacagac
     5941 gatagccggg gtgcagccgg tggatcaggt agccgccgag ctccatgtcg ttgaagatcg
     6001 gcgcgggcag gcgctcgcgt tcgatgaaat cggcggccgc cgccgcggtc gatcccggcg
     6061 cgagcgaagc ccaggtgtcg cgcgattgcc ccaactcgaa cgccggccgc agccgccaca
     6121 gctcggccgc ggcggcgaga cacagcaggc agaaggtggc ggcggcgaaa ccgtgcgcca
     6181 cgcgccacgg cggtcgccgc ggcgccgagc cccgccaacc gcgatcgtgc aggttcaaca
     6241 cgaccacgac ggcccaaacg acggcgaacg ccggaacgaa ccgcagcgcg aacagtccga
     6301 tcacgccgaa gcccccgagc agcagcgccc gcgagccgag cctcggcgag cgcaggcgaa
     6361 tcaggctggc cgcaccgctc gtcgcgcaga gcagccagat ccacggctca cgcagcgcca
     6421 actgctcgaa cgtcgggcgc cactcgagca ggtagccgtg acgcgacatc accatgaagg
     6481 cgtaacccag ctcgccgtgc cacagcgacg gcgtgatcag cgacacggcg gcgaacgccg
     6541 gcggccacca ccaggccggc cgacgcgggt cgcccgacag ccatcccccg gcggcgtagg
     6601 ccaacacgat caggatgccg aggtagaagc caccgtgaag attggcccac agccactgga
     6661 tggccagcat cgcgagcaac gtgcgcgagg tcggcttgcg atcgcgttcg agcagcagga
     6721 cgaagagcgc cgcgagcagc cagccggcga cctcggtgcg cagcagcagc cgctggttgc
     6781 cggcggacag cacgaccagt gcgagcgcgc cgtcgagcag gccgaagcgg tcgagcggtc
     6841 cgcgggcgaa caccagcacg acgagcaccg cgaccagcag ccccgacagc agcgacagac
     6901 cgcgcagtcc ggccagctcg tcgaccgccg cgacgaccac cgtccacaac caggagtggg
     6961 tgacccaccc cgcggcctcg cccgtcgcca ggaggaacgt gtcgcggtcg ggaacccacc
     7021 gctcggcgag gatgcgccgc ccgctggcga ggtaccacca caggtcctcg ttccacagat
     7081 aggccagcgc ctgagacagg ccgagcgcca gtgtcgcgct cgccaccgga atcgcggcga
     7141 cgcgcctcgc gccgaccacc gccggcgacg gccgccccgg ctcagctacc gcggaggatg
     7201 ttgccgagga tcccaccgac gtccccacgc ccgccgctcg aggtgcgcga ggtcggcagt
     7261 cgcgggagca gccagtcgag aaacgcctgg ttcgaccgtg tctgcagcca gaccttgccc
     7321 cggccgctga actcgacgac caacccctcg cccgagatga gcgaggtgaa cagcccggaa
     7381 accttgcgca cgtcccagct cacggacggc tcgaacgcga ccacgtgtcc ggtatcgacc
     7441 atcagcgacc gcgcgccgtc gagctcgacg cggtggatcg cgccgaacga gctgagcatc
     7501 acctcgccct gtccgcgcag gtggagcagg aacaggcctt cggcggcgaa gaacgaacgc
     7561 gcgccgcccc acttgatgtc gatgtcgatc gtcggtccgc cggcgagata cgagccggac
     7621 tgcacgtaca gctcgccgtt cagccggacc tcgtcgacgt cgccgggaag actgggcgcg
     7681 aaagtcactt cgcccgggcc gccgtcggcg gtgaaggtgt tgacgaagaa cgactcgccg
     7741 ccgagcacct tgcgcagcgc cgacttgaac aaaccgccct gcattccact ctcgacgcgc
     7801 acgttgccgc tcatcgacac catcgcgccg gtttccgcct tgaccgactc tccactctcg
     7861 agatcgacga tcgcgagcgc gtaggacgga cgatgctcga ccctggttc
//
LOCUS       DASPGN010000011        12901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_704544, whole genome shotgun sequence.
ACCESSION   DASPGN010000011 DASPGN010000000
VERSION     DASPGN010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12901
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704544"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /locus_tag="VD788_00320"
     CDS             complement(<1..1806)
                     /locus_tag="VD788_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV74735.1"
                     /translation="MGILRAASLACFILASAAHADEMPATARSCWPGEPGTDPRACAP
                     NDEGYRDAWEFWSTIPESIDLERMHPAERALGSIGISLDRAWQHTIGRDDVVIAVLDS
                     GIRWDYADLINKIYINRGELPPPAGSTGHDADGNGLFDVRDYHGDQRVGDRNRNGVID
                     AGDLILAFSDCEDNDGNGYPDDIAGYDFFGRDHCGFEGGDNDPFDVTDFGHGTGIGST
                     AAAETDNLVRDAGVCPRCRVLPVRVGDSFVVDVNRFARGLTYAVRAGASVVASALGSY
                     NNTPAGREAIDLAYRHGVPVIASAADEFAYHHNYPSVYNHALYVNAIRFNHADDYREA
                     STFWGVNPCTNFGARVWITVPAESCSSGATSRLAGVAGLVESIARDAGHGDLHAEEVY
                     QILRASADDLDNTDPDWGAMRYTARPGFDQFYGYGRLNAWRAVTMAEQGRIPPIADLA
                     DPDWFAVVSPKREPRLKIHGTIETRGKRPASYRLEYGLGVEPAEGDYVKVAEGSIEGS
                     YSGRLGVLDFTRLPLPTGPSPRDRDERDRYSVTLRLSVADGRGPEAEARRSFFVFDDP
                     SWKTHFPLALGASGEAGPLLVDLDGDGRDEIVLPTA"
     gene            1871..2803
                     /locus_tag="VD788_00325"
     CDS             1871..2803
                     /locus_tag="VD788_00325"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HXV74736.1"
                     /translation="MGGERNVSIRLAIAVLGAAAACHVPAVAGGLEPIATLGHPPLGE
                     VSGIARSSRPGVFWVHNDSGDRARVFAVTLDGEPVVPIDPDRRRAKRDWPGVELPGAT
                     NVDWEDIAFEDGTLYIADTGNNGNARRDLGLYLVREPDPHAAHRTEAVRFLPLRYPDQ
                     TAYPAEDWEFDCEALFVDRGTPYFLTKHRAAGQWLGFLPGTKLYRLDTRSTEDNVLTL
                     VGKRADLMMPTAADLSPDGRQLAVLTYMDLWIFDRPAEGDDWLSGPARRLPLDRARAR
                     TNEAIAWADDRTVIIANEERRLFRLDVAELEPPD"
     gene            complement(2812..3090)
                     /locus_tag="VD788_00330"
     CDS             complement(2812..3090)
                     /locus_tag="VD788_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74737.1"
                     /translation="MRSATLKITVVAVGFAAATVGVLVFAMLGQAQVECEVCITFHGR
                     TQCRQAAGPDRESAIRTATDNACAFLAAGMSDSISCANTPPDVVTCDD"
     gene            3305..3541
                     /locus_tag="VD788_00335"
     CDS             3305..3541
                     /locus_tag="VD788_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74738.1"
                     /translation="MERLGTIRLSPDGTCTLDTPGRELQFLDARVGHNVLLFLIAWND
                     VGVFSVDTQQALEALRRKHRIRRVLRAPPASPVD"
     gene            3698..3856
                     /locus_tag="VD788_00340"
     CDS             3698..3856
                     /locus_tag="VD788_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74739.1"
                     /translation="MEVPRERYREIERVIASEESPVGIDAKKTHVIIIHKLTEIERRL
                     AELERKIG"
     gene            complement(3876..4487)
                     /locus_tag="VD788_00345"
     CDS             complement(3876..4487)
                     /locus_tag="VD788_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74740.1"
                     /translation="MVSMPARRALASALVVVSAACSAPLVWSAGRDSVVVTGGRLAGQ
                     PIRPDRWVIRVVAGGPIAGDVELVAFRAGDETDRPGELGYTWTWGDRRESIVLVGDLA
                     PGANELRAVLELAAPPSAGTYYILFGHASGVDLARAFSGTGSLDDPPLWGDGNDMHDL
                     DGDDLSFARRNGYVGDWLRLVAGDYEFGRIAVAPVRVVVLPAK"
     gene            complement(4578..6809)
                     /locus_tag="VD788_00350"
     CDS             complement(4578..6809)
                     /locus_tag="VD788_00350"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HXV74741.1"
                     /translation="MRQLSDRPTVSLGFLVVGLATLAYGSGPTDGDSRQPTDGQRRML
                     QLFSKIRELTPSQNPYLGDERTRGLRRQVAAFPADVPVLERVRYHLKLGRDELRLGNP
                     TDAIAQYTAAMALVPGLDPAQQRVVRNMLSYHLGVANLRWGENLNCVSRHTADSCLLP
                     IRGGGVHTDQTGSRAAIEYFNVVLENSSPDSDEYVAARWLVNIAFMTIGEYPDAVPQR
                     FRIDPAVFASDEPFPRFVNEAAELGLDRFDLSGGAIADDFDGDQDFDLVVSSWDPAGG
                     LHYYRNDGGGRFAEQTADAGLAPLIGGLNLVHADYDNDGDLDLLVLRGAWLRGEFARQ
                     PNSLLRNDGAGRFVDVTFEAGLGEVHHPTQTAAWADFDLDGDLDLYVGNESTADTAHP
                     GQLFRNEGDGTFLDVAVRAGVQNFGYAKGVAWGDYDADGYPDLYVSNLASANRLYHNN
                     GDGTFTDVADRAGVALPLDSFPTWFFDFDNDGALDLFVAGYDQADGPSRLAGVAASYL
                     GLPHDAETHRLYRGDGRGGFTDVTAQTGLDRVAMTMGANYGDLDNDGFLDFYLGTGYP
                     DYEALMPNVMYRNVGGRKFADVTTAGGFGHLQKGHAVVFADLDGDGDQDVFEQMGGAY
                     PGDAFGNVFYRNPGHGRNHVELRVVGVRSNRFGLGTRVRLAIVEGETRREIHRHVASG
                     GSFGANPYTLHVGIGQATRVESIEVFWPATGATQRFGSVPAGARVVVTEARDRLQIAE
                     VGG"
     gene            complement(6943..8214)
                     /locus_tag="VD788_00355"
     CDS             complement(6943..8214)
                     /locus_tag="VD788_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV74742.1"
                     /translation="MSGSGADPIAFLAAIPAFDGLPRELLGAMHERMEPRTFDKGDLL
                     IRQDDTGEFLLVLTEGTADVSVRQSDGTRRTIGRVKAGDIVGEMALITGESRGADVEA
                     RERVRALALAATAFDRLARRTPLLGVVLTNLIADRLGDTSGDVLGGKTLNGYRIDRCV
                     GRGGMAVVYEARRDADDRLVALKMMSHRLVYDSAALSRFRQEADVVERLEHENIARVF
                     SRFSAFGTHFLVMEFLDGTTLDALRERGKPMPEALARPMVGQLSRALGYVHGRGMVHR
                     DLKPGNVMITVDGRVKLMDFGLVKPLVITDERTATHEMKLVGTPSYMAPEQFSGEEFD
                     HRVDIYALACLTLSMLLGEKVFRATNLLDLARRKLAYGVPPRREIGGGVSEEMHEFIV
                     RGMDPRQERRLASLVAYEGWAGPVDLSALDD"
     gene            8368..10716
                     /locus_tag="VD788_00360"
     CDS             8368..10716
                     /locus_tag="VD788_00360"
                     /inference="COORDINATES: protein motif:HMM:NF041328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74743.1"
                     /translation="MLGKRPSSVRAFVVFGGALLVPMAGIPVVLSAEQAIDLDGNPLN
                     GKESRVVTRVLQSYPVKIENVIYNNSHGKAFIFDWSGAGPGGFGSFVVAGPDVGTKWE
                     WTTLSQVYSILSPATFAPEDRGLPTVGGQPGGIQAGGDPGVFSLPGKSIYPTTVTVSS
                     ASLTSSLVTFFSPEQEVASCGGSFSAGRFEQTISNRSGGAVSFSVEQQDCCPEPQMTI
                     CQDGCRFYLTDPDNCGGCGIECGEGEICSAGSCELVCTGGTTECDGACVDLSNDPLNC
                     GACGNQCEADQFCGEGTCQDICAPGLTLCGDLCVDLTSDPLNCGACGNTCGSNDICAA
                     GLCETCRPPRPTACDNECVNTNTDPYHCGGCGMACDFTNCPSTGSGTCSQGTSCVCLE
                     SGGSTAEAVLYSPIDIPGALEPRAPRRQRIGRAGPRAPAAIEAAATRGLQAIEAPVCD
                     LEPLEMTIEDGETFSRCLDGARIGKEVFTTAQIQRDGEIIGGGPCAVIVPDTQAVIPP
                     FLPAPVDVDVLDQSGDGLLQPGESGTLWLTVANLGDSPFDDPVAVLSSPPDSFNPAPV
                     VFANDASAYGGFPAFVENGDCTNEPQFESRTNLVGFEVTIPEEQEADVGRVFQLSLEG
                     SADGPAVVMPVVIGIGAQCDPAVTLDGETFDGLAGFLEPVNAMLRPRNGDPQFSPGDF
                     NRGKTVPLKMRLICGERTLGPTEIDPTPEIVGLVHDSLGPQPLAAINADNGANPDNPF
                     FVCGTNRCEFQLRTTDLPVGDLVIEVKLPDSRVFHAGFTLRP"
     gene            10760..12811
                     /locus_tag="VD788_00365"
     CDS             10760..12811
                     /locus_tag="VD788_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74744.1"
                     /translation="MIVLPTLVLAAAGAFAAPLPARVVDVQADDPRGGEPPELPFGIR
                     GWGQGAARVAAPSASSFSLETPADRAVTVAQRISGATMAGDVVGFALAPGRTTAVYIA
                     DQEVAGRFELYSAPLDASAAPVKISTGLTFDGAEGVSAFRITPDGTRVVFLADPDGGP
                     GQDDLYSVPIGGGSTPVRLNASGQRPVTAFGLAPNSATVVYFGVGTTGAFELFAATIG
                     SAASGIQLSDVGSTNPAGSVVFATIGPDSARAVYAADPVTTGVFQWHSVSLAAAGPGT
                     DATLSAGLEGVGLHAIRPDGARVAYTADENLAGVFELFEIPIAGGARIRLNPAMAGAG
                     VRALEYSPDGSRIAYLADQNTSDVVEVYGAQSGAAGSGARVSNPMFGTQSADTLNVGP
                     DNATVLFEADANVDETFELFHATLGSAAGSATLYPVSAPDNAGSFGDRGTPIIGDRAV
                     FPLLGTTVDLIGVPFDGSAPARVINDELATGQTVRNAFLPPAATTLLMFGSGPDGATS
                     TDRVWVASIARDLPQEQINATAGAGTIGVTGYAIDDGQAYAVYAQDQQTAGKIELFSR
                     PLDSDADGVASPDDNCPFDANASQQPVVFAQTVFATGTHGFGWAQPTDARWVRGPLDQ
                     LDVYATNGSGTAVGAVAFTDETSPAPGSGLYYLFAPDCAGRSYQTAPGGEPARDLATF
                     P"
BASE COUNT         2014 a         4438 c         4489 g         1960 t
ORIGIN      
        1 ggccgtcggc agcacgatct cgtctctccc atcgccgtcg agatcgacca gcagtggacc
       61 ggcctcgccg gaggcgccga gcgcgagcgg aaaatgggtt ttccacgacg gatcgtcgaa
      121 gacgaagaag ctgcgccgcg cctcggcctc ggggccgcgc ccgtcggcga ccgagagccg
      181 cagcgtcaca ctgtatcgat cgcgctcgtc ccgatcgcgc gggctggggc cggtgggcag
      241 cggcagtcgc gtgaagtcga ggacgccgag tcggccgctg tacgatccct cgatcgagcc
      301 ttccgccacc ttgacgtagt cgccctcggc gggctcgacg ccgagcccgt actcgaggcg
      361 gtagctcgcc gggcgcttgc cgcgcgtctc gatcgtgccg tggatcttca accgcggctc
      421 gcgtttcggt gagacgacgg cgaaccagtc agggtcggcc agatcggcga tcggcgggat
      481 ccgcccctgc tcggccatcg tcaccgcacg ccacgcgttg agacggccgt aaccgtagaa
      541 ctggtcgaag ccggggcggg ccgtgtagcg catggcgccc cagtccggat cggtgttgtc
      601 cagatcgtcg gccgacgcgc gcaggatctg atagacctcc tccgcgtgca ggtcgccgtg
      661 accggcgtcg cgcgcgatcg actcgaccaa ccccgcgaca ccggcgaggc gcgacgtcgc
      721 acccgaggag cagctttccg ccggcaccgt gatccacacg cgagcgccga agttggtgca
      781 cggattgacg ccccagaagg tcgatgcctc gcggtagtcg tccgcatggt tgaaacggat
      841 cgcgttgacg tagagcgcgt ggttgtagac gctcgggtag ttgtggtggt aggcgaattc
      901 gtcggccgcg gacgcgatca ccggaacgcc gtggcggtag gcgaggtcga tcgcttcgcg
      961 cccggccggc gtgttgttgt agctgccgag cgccgaggcg acgaccgatg cgccggcccg
     1021 tacggcgtag gtcaagccgc gcgcgaagcg gttgacgtcg acgacgaagc tgtcacccac
     1081 gcggacgggc agcacgcggc aacgcgggca gaccccggca tcgcggacga ggttgtcggt
     1141 ctcggcggcc gcggtcgagc cgatgccggt gccgtgaccg aagtcggtga cgtcgaacgg
     1201 gtcgttgtcg ccaccctcga agccgcagtg gtctcggcca aagaaatcgt agcccgcgat
     1261 gtcgtcgggg tagccgttgc cgtcgttgtc ctcgcagtcg gagaaggcga ggatcaggtc
     1321 gcccgcgtcg atgaccccgt tccggttccg atcgccgacc cgctggtcgc cgtggtagtc
     1381 gcggacgtcg aacagcccgt tgccgtcggc gtcgtggccg gtcgacccgg cggggggcgg
     1441 cagctccccc cgattgatgt agatcttgtt gatcagatcg gcgtagtccc agcggatccc
     1501 gctgtcgagc acggcgatga cgacgtcgtc gcggccgatc gtgtgctgcc aggcgcggtc
     1561 gagcgaaatg ccgatcgagc cgagcgctcg ttcggcggga tgcatccgct cgagatcgat
     1621 cgactcggga atcgtgctcc agaactccca ggcgtcccga tagccttcgt cgttgggtgc
     1681 gcaggcccgg gggtcggtcc cgggctcgcc cggccagcac gagcgggcgg tcgccggcat
     1741 ctcgtcggcg tgcgccgcgc tcgccaagat gaagcatgcg agcgacgccg cgcggaggat
     1801 ccccatgcac gggaatctag gccggcgccc gggcggcgca acccgcggcg aagtagctta
     1861 tgctgtccga atgggtggcg aacggaacgt ctcgatccgg ttggcgatcg ccgtgctcgg
     1921 tgcggcggcg gcgtgccacg tgcccgccgt cgcgggaggt ctcgagccga tcgctacact
     1981 cggccatcca ccgctgggcg aggtcagcgg catcgcccgg tcttcccggc cgggcgtgtt
     2041 ctgggtgcac aacgacagcg gcgaccgggc ccgcgtcttc gccgtgacgc tcgacgggga
     2101 gcccgtcgtc ccgatcgatc ccgatcgccg gcgggcgaag cgcgactggc ccggcgtcga
     2161 gctgccgggc gcgaccaacg tcgactggga agacatcgcg ttcgaggacg gcacgctcta
     2221 catcgccgac accggcaaca acggcaacgc gcgtcgcgat ctcggtctgt acctggtgcg
     2281 cgagccggat ccgcacgccg cgcaccgcac cgaagccgtc cggttcctgc cgctgcgcta
     2341 ccccgatcag acggcctacc cggccgagga ctgggaattc gactgcgagg cgctgttcgt
     2401 cgaccgcggt acgccgtact tcctgaccaa gcatcgcgca gccggtcagt ggctcggctt
     2461 ccttccgggg accaagttgt accggctcga cacgcgatcg accgaggaca acgtgctgac
     2521 gctggtcggc aagcgggcgg acctgatgat gccgaccgcc gccgatctgt cgcccgacgg
     2581 caggcagctc gccgtgctga cctacatgga tctctggatc ttcgaccgtc cggcggaggg
     2641 cgacgactgg ctgtccggac cggcgcgacg cctgccgctc gatcgcgccc gcgcgcggac
     2701 gaacgaggcg atcgcctggg ccgacgaccg caccgtgatc atcgccaacg aagaacgacg
     2761 cctgttccgg ctcgacgtcg ccgagctcga gccgccggac tgaaatcggc gtcagtcgtc
     2821 gcaggtcacg acatcgggcg gcgtgttggc gcagctgatg ctgtcggaca tcccggcggc
     2881 gaggaacgcg caggcgttgt cggtcgcggt gcggatcgcc gattcccgat cgggcccggc
     2941 ggcctgcctg cactgcgtcc ggccgtgaaa ggtgatacag acctcgcatt cgacctgcgc
     3001 ctgccccagc atggcgaaaa cgagcacgcc gacggtggcg gcagcgaagc cgacggcgac
     3061 gaccgtgatc ttgagcgttg ccgagcgcat ccagcttcat ggtaggaggg gcaggcgacg
     3121 atgtaaaggc atcgcgaatc gcgacgggtc aagatcgccg accggcgcag tcgtcgtcgg
     3181 cctcggtttc cagccacgac cggcaacgtt ccctgagggg tccccagggt tgaacgtcgc
     3241 gcccccggcc cggagaacgg aattatgccg atcggtccac gagcgtttca tcaaggaccg
     3301 tccggtggag aggctcggca cgatccgtct gagcccggac gggacttgca cgctcgatac
     3361 gccgggtcgg gagctgcagt tcctggacgc gcgcgtcggc cacaacgtgc tcctgttcct
     3421 gatcgcatgg aacgacgtcg gcgtgttctc ggtggacacc cagcaggcgc tggaagcgct
     3481 gcgccgcaag catcggatcc ggagggtgct acgcgcgcct cctgcgtccc cggtagattg
     3541 acgccctgct cgggagcgcg taggctccgg gaatcggacc aagcatgccg ttttgaactg
     3601 ggtgtccacg aaaccgccgg aggcccggtt cacgacggcg atcgacacag ccgggcccgg
     3661 gccgctgccg agcgtacgtc tttcggagga acggagaatg gaagtaccga gggagagata
     3721 ccgggagatc gaacgggtga tcgcgagcga ggagagcccc gtcggcatcg acgcgaagaa
     3781 gacgcacgtg atcatcattc acaagttgac ggagatcgag cggcggctcg cggagctcga
     3841 acggaagatc ggctagcgtc tgaggccgga gcggctcact tcgcgggcag gaccaccacc
     3901 cgcaccggtg cgacggcgat ccggccgaac tcgtagtcgc ccgccaccag ccgcagccag
     3961 tcgccgacgt agccgttgcg ccgggcgaac gacaggtcgt cgccgtcgag atcgtgcatg
     4021 tcgttgccgt cgccccacag cggcgggtcg tcgagcgacc cggttcccga gaaggcgcgc
     4081 gccaggtcga caccgctcgc gtggccgaac aggatgtagt aggtcccggc cgacggcggc
     4141 gcggcgagct cgagcaccgc ccgcagctcg ttcgcccccg gagcgagatc gccgacgagc
     4201 acgatcgact cgcggcgatc gccccaggtc caggtgtagc ccaactcgcc gggccgatcg
     4261 gtctcgtcgc ccgcgcggaa cgccaccagc tcgacgtcgc cggcgatcgg gccgccggcc
     4321 acgacccgga tcacccaacg atcgggacgg atcggttgac cggccaatcg gccgccggtc
     4381 accaccacgc tgtctcgccc cgccgaccag acgagcgggg cggaacaggc cgccgacacg
     4441 acgacgagcg cggaggccag cgcccggcgt gcgggcatcg agaccatcgt ttccctcccg
     4501 ggtgggacgg gcgtaattgt acgcaaatcg agcgaacgcg gggtggagtt tcgcggggcc
     4561 gtcggggtct ggacggtcta cccgccgacc tcggcgatct ggagccgatc gcgggcttcg
     4621 gtgacgacga cgcgcgcgcc cgccggcacg ctgccgaatc tctgcgtggc gccggtcgcc
     4681 ggccagaaga cctcgatcga ctcgaccctc gtcgcctggc cgatgccgac gtgcagcgtg
     4741 tagggattgg cgccgaagct gccgccgctc gcgacgtgac ggtggatctc acgccgcgtc
     4801 tcgccctcga cgatcgcgag acgcacccgc gtaccgagtc cgaaccggtt cgagcgtacg
     4861 ccgaccactc gcagctcgac gtgattccgc ccgtgccccg ggttgcggta gaacacgttg
     4921 ccgaacgcgt cgccggggta ggcgccgccc atctgctcga agacgtcttg gtccccgtcc
     4981 ccgtccaagt cggcgaagac gaccgcgtgg cctttctgga ggtgaccgaa accgccggcg
     5041 gtggtcacgt cggcgaactt cctcccgccg acgttgcggt acatcacgtt gggcatcagc
     5101 gcttcgtagt cggggtagcc ggtgccgagg tagaaatcga gaaacccgtc gttgtcgaga
     5161 tcgccgtaat tggcgcccat cgtcatcgcc acccgatcga gacccgtctg ggcggtcacg
     5221 tcggtgaacc cgccgcgacc gtccccgcgg tacagtcggt gcgtttcggc gtcgtggggc
     5281 aggccgaggt agctcgcggc gacgccggcc agcctcgacg gtccgtcggc ctggtcgtaa
     5341 ccggcgacga acagatcgag cgcgccgtcg ttgtcgaaat cgaagaacca cgtcgggaag
     5401 ctgtcgagcg gcagcgcgac gcccgctcgg tcggcgacgt cggtgaacgt gccgtcaccg
     5461 ttgttgtggt agagacggtt ggccgaggcc aggttcgaca cgtagagatc gggatagccg
     5521 tcggcgtcgt agtcgcccca ggccacgcct ttggcgtagc cgaagttctg gacgccggca
     5581 cggaccgcga cgtcgaggaa cgtcccgtcc ccctcgttgc ggaacagttg acccgggtgg
     5641 gccgtgtcgg cggtcgactc gttgccgacg tagaggtcga gatcgccgtc gaggtcgaag
     5701 tcggcccacg ccgcggtctg cgtcgggtga tggacctcgc ccagccccgc ttcgaacgtc
     5761 acgtcgacga accgccctgc gccatcgttg cgcagcaacg agttgggctg gcgggcgaat
     5821 tcgccgcgca gccaagcccc gcgcagcacg agcaggtcga ggtcgccgtc gttgtcgtag
     5881 tcggcgtgca cgaggttcag tccgccgatc aacggggcga gaccggcatc ggccgtttgc
     5941 tcggcgaacc tgccgccgcc gtcgttgcgg tagtagtgca aaccgcccgc cggatcccag
     6001 ctcgacacca cgaggtcgaa gtcctggtcg ccgtcgaagt cgtcggcgat cgccccaccg
     6061 ctaaggtcga accggtcgag tcccagctcg gcggcctcgt tgacgaaacg ggggaacggc
     6121 tcgtccgacg cgaagaccgc cggatcgatc cgaaagcgct gcggcaccgc gtccggatac
     6181 tcgccgatcg tcatgaacgc gatgttgacc agccagcgcg cggcgacgta ctcgtccgag
     6241 tcgggcgagc tgttctcgag caccacgttg aagtactcga tcgccgcacg cgatccggtc
     6301 tggtcggtgt ggacgccgcc gccgcgaatc ggcaacagac agctgtcggc ggtatggcgc
     6361 gagacgcagt tgagattctc gccccagcgc aagttggcca cgccgaggtg gtaggacagc
     6421 atgttgcgca cgacgcgctg ctgcgcgggg tcgagcccgg gcacgagcgc catcgccgcg
     6481 gtgtattgcg cgatcgcgtc cgtcgggttc ccgagacgga gctcgtcgcg cccgagcttg
     6541 aggtgatagc gcacccgctc gagcacgggc acgtcggccg ggaaggcggc gacctgccgg
     6601 cgcaacccgc gtgtacgctc gtcaccgagg tacggattct gcgacggcgt cagctcgcgg
     6661 atcttgctga acagttggag catccgtcgc tggccgtcgg tcggctggcg cgaatcgccg
     6721 tcggtcggtc cgctcccgta tgccagcgtc gcgagaccca ccacgaggaa cccgagcgat
     6781 acggtcggcc gatcggagag ctggcgcaat gatggtccac gcattcgcga cggagcgatg
     6841 caccgtttta tacgcgtcgg agcggggggc agtttccgca gagcccgaaa acggcgccag
     6901 gattctgtcg ccgcctttgt aacagcggtg taaacacggg cgtcagtcgt cgagtgcgct
     6961 aaggtcgacc gggccggccc atccttcgta ggccacgagc gacgcgaggc gacgctcctg
     7021 ccggggatcc atcccgcgga cgatgaactc gtgcatctct tcgctgaccc caccgccgat
     7081 ctcacgcctc ggcgggacgc cgtacgccag cttgcgccgg gccagatcga gcaggttggt
     7141 cgcgcggaac accttctcgc cgaggagcat cgacagcgtc aggcaggcca gggcgtagat
     7201 gtccacccga tggtcgaact cctcgccgga gaactgctcg ggagccatgt aggacggcgt
     7261 gccgaccagt ttcatctcgt gcgtcgccgt ccgctcgtcg gtgatcacga gcggcttgac
     7321 cagtccgaaa tccatcagct tgacgcggcc gtcgacggtg atcatcacgt tgccgggttt
     7381 gaggtcccgg tggaccatgc cgcggccgtg gacgtagccc agcgcccgcg acagctggcc
     7441 gaccatcggt cgcgccagcg cttccggcat cggcttgccc cgctcgcgca gcgcgtcgag
     7501 cgtcgtccca tcgagaaact ccatgacgag gaagtgggtc ccgaacgcgg aaaagcggct
     7561 gaacaccctg gcgatgttct cgtgctccag tcgctcgacg acatctgcct cttgtcggaa
     7621 gcgggacaac gccgccgagt cgtagaccag ccggtggctc atcatcttca gcgccacgag
     7681 ccgatcgtcc gcgtcgcggc gcgcttcgta gacgaccgcc atcccgccgc gcccgacgca
     7741 gcgatcgatg cgatagccgt tcagcgtctt gccgccgagc acgtcccccg acgtgtcgcc
     7801 cagccgatcg gcgatcaagt tggtcagcac gacgcccaac aggggcgtgc gtcgcgccag
     7861 ccggtcgaac gccgtcgcgg cgagggccag cgcccggacg cgctcccgcg cctcgacgtc
     7921 ggcgccgcgc gactcgcccg tgatcagcgc catctcgccg acgatgtctc ccgccttgac
     7981 ccgaccgatc gtccgccggg tcccgtcgct ctgccgcacc gagacgtcgg cggtaccttc
     8041 tgtgagcacc agtaggaact caccggtatc gtcctgccgg atcagcaggt cgcccttgtc
     8101 gaacgttcgc ggctccatcc gctcgtgcat cgcccccaac agctcccgcg gcagcccgtc
     8161 gaacgccgga atcgcggcga gaaaggcgat cggatcggca ccagacccgc tcatgcccgc
     8221 atggtacacg aacaccggac gaaacccgga cggcctcgat acgccaagtt cgacatgggc
     8281 agcgggagcg gttctcggta caatctcgtc caggcggggc ctgggggcag tcggccgcac
     8341 aagccaggct tcattcgtgg gggagctatg ctcggcaagc gtccgtcgtc cgtgcgtgcg
     8401 ttcgtcgtct tcggtggggc actgctggta ccgatggcgg ggatcccggt cgtcctctcc
     8461 gccgagcagg cgatcgatct cgacgggaat ccgctgaacg gcaaggaaag ccgagtggtg
     8521 acccgggtcc tgcagagcta cccggtcaag atcgagaacg tcatctacaa caactcgcac
     8581 ggcaaggcat tcatcttcga ttggtcgggc gccgggcccg gcgggttcgg ctcgttcgtc
     8641 gtcgcggggc cggacgtcgg taccaagtgg gagtggacga cgctgtcgca ggtctattcg
     8701 atcctcagcc cggccacctt cgccccggag gaccgcggcc tacccaccgt cggcggccag
     8761 ccgggcggca tccaggccgg cggcgacccc ggcgtcttct cgttgccggg caagtcgatc
     8821 tacccgacga ccgtcaccgt gtcgagcgcg agcctgacct cgagcttggt gaccttcttc
     8881 agccccgaac aggaggtcgc cagttgcggc ggctcgttca gcgccggccg cttcgagcag
     8941 acgatcagca accgctcggg cggcgcagtc agcttctcgg tcgagcagca ggactgctgc
     9001 cccgagccgc agatgacgat ctgccaagac gggtgccggt tctatctgac cgacccggac
     9061 aactgcggtg ggtgcgggat cgagtgcggg gaaggggaga tctgctccgc cggatcctgc
     9121 gagctggtct gcaccggcgg cacgaccgag tgcgacgggg cgtgcgtcga cctgtcgaac
     9181 gacccgctca actgcggtgc ctgtggcaac cagtgcgagg ccgaccagtt ctgtggcgag
     9241 ggcacgtgtc aggatatctg tgcgccggga ttgacgctct gcggcgacct gtgcgtcgac
     9301 ttgacgagcg acccgctcaa ctgcggcgcc tgcggcaaca cgtgcggctc gaacgacatc
     9361 tgcgccgccg gtctgtgcga gacctgccgg ccgccccggc cgaccgcctg cgacaacgag
     9421 tgcgtcaaca ccaacaccga cccgtaccac tgcggcggtt gcggcatggc ttgcgatttc
     9481 accaactgcc cgtcgaccgg cagcggaacg tgcagtcagg gaacttcgtg cgtgtgtctc
     9541 gagagcggcg gctcgaccgc cgaggcggtg ctgtattcgc cgatcgacat tccgggcgcg
     9601 ctcgagccgc gcgcgccgcg ccggcaacgc atcgggcgag cggggccgcg cgccccggcg
     9661 gcgatcgagg cggcggcgac acgcggcttg caggcgatcg aggctccggt ctgcgatctc
     9721 gagccgctcg agatgacgat cgaggacggc gagacgttca gccgctgcct cgacggagcg
     9781 cgcatcggca aggaggtctt cacgaccgcg cagatccaac gcgacggcga gatcatcggt
     9841 gggggaccgt gtgcggtgat cgtacccgac acgcaggccg tcattccgcc gttcctcccc
     9901 gcgccggtcg acgtcgacgt gctcgaccaa agcggtgacg ggctgctgca gcccggcgag
     9961 tcgggcacgc tgtggctgac cgtcgccaac ctgggcgatt ctccgttcga cgacccggtc
    10021 gccgtgctga gcagccctcc cgactcgttc aatcccgcgc cggtggtctt cgccaacgac
    10081 gcatcggcct acggcggctt cccggcgttc gtcgagaacg gggattgtac gaacgagccg
    10141 cagttcgagt cgaggaccaa cctggtcggc ttcgaagtga cgatccccga ggagcaggag
    10201 gccgacgtcg gccgcgtgtt ccagctgtcg ctcgagggta gcgcggacgg gccggcggtc
    10261 gtgatgccgg tcgtgatcgg catcggggcc cagtgcgacc ccgccgtgac gctcgacggc
    10321 gagaccttcg acgggctcgc agggttcctc gagccggtca atgcgatgct gcggccgcgg
    10381 aacggcgacc cgcagttctc gcccggcgat ttcaaccggg gcaagaccgt tccgctgaag
    10441 atgaggttga tctgcggcga gcgcacgctg gggccgacgg agatcgaccc gacacccgag
    10501 atcgtcgggc tcgtgcacga ctcgctcgga ccgcagccgc tcgcggcgat caatgcggac
    10561 aacggtgcga accccgacaa cccgttcttc gtctgcggta cgaaccgctg cgagttccag
    10621 ctgcgaacga ccgatctccc ggtcggcgat ctcgtgatcg aggtcaagct gcccgactca
    10681 cgcgtgttcc acgccggttt caccctgcga ccctgagcga ataccgatgc gacgaacgac
    10741 atcacgaagc tgcacagcgt tgatcgtcct gccgaccctg gtcctcgccg ccgcgggcgc
    10801 gttcgccgcg ccgctgccgg cacgggtcgt cgacgtccaa gccgacgacc cgcgcggtgg
    10861 tgagccgccc gagcttccct tcgggatccg cggctggggt cagggcgcgg cgcgagtcgc
    10921 cgcgccgtcg gcgtcgtcgt tctcgctcga gaccccggcc gatcgagcgg tcaccgtcgc
    10981 gcagaggatc tccggggcga cgatggcggg cgacgtcgtc ggcttcgccc tcgcccccgg
    11041 tcggacgacg gcggtctaca tcgcggacca ggaggtcgcg ggtcggttcg agctgtacag
    11101 cgccccgctc gacgcgtcgg cggcgccggt gaagatcagc accgggctga cgttcgacgg
    11161 cgcggagggt gtcagcgcgt tccgcatcac gcccgacggc acgcgcgtcg tgttcctcgc
    11221 cgatcccgac ggcggccccg gccaggatga cctctacagc gtgccgatcg gcggtggctc
    11281 gaccccggtg cggctgaacg cgtccggcca gcgtccggtc acggcgttcg ggctggcgcc
    11341 gaacagcgcg accgtcgtct acttcggtgt cggcaccacc ggggcgttcg aactgttcgc
    11401 cgcaacgatc ggctcggccg cgtccggcat ccagttgagc gacgtagggt cgaccaaccc
    11461 ggcgggctcc gtcgtcttcg ccacgatcgg tcccgacagc gcacgcgcgg tctacgccgc
    11521 cgatccggtg acgaccggcg ttttccaatg gcacagcgtt tcgttggccg ccgcggggcc
    11581 gggaacggac gcgacgctca gcgccgggct agagggcgtc ggattgcacg cgatccgccc
    11641 ggacggcgcg cgtgtggcat acaccgccga cgagaatctg gccggcgtgt tcgagctgtt
    11701 cgagatcccg atcgcgggcg gggcgcggat ccggctgaat ccagcgatgg ccggtgccgg
    11761 ggtgcgcgcg ctcgagtaca gccccgacgg cagccgcatc gcctatctcg ccgaccagaa
    11821 cacgtcagac gtggtcgagg tgtacggcgc ccagtcgggc gcggccggat cgggcgcccg
    11881 ggtgagcaac ccgatgttcg ggacgcagtc ggcggatacg ctgaacgtcg ggccggacaa
    11941 cgcgaccgtg ttgttcgagg ccgatgcgaa cgtcgatgag acgttcgagc tgttccacgc
    12001 cacgctcggc tcggcggccg gctcggcgac gctctatccc gtcagcgcac cggacaacgc
    12061 gggtagcttc ggcgatcgcg gcacgccgat catcggcgac cgcgcggtct tcccgctgct
    12121 cggaacgacg gtcgatctga tcggggtccc gttcgacggc agcgcaccgg cccgcgtgat
    12181 caacgacgag ctggcgaccg ggcagaccgt tcgcaacgcg tttctcccgc cggcggcgac
    12241 gacgctgttg atgttcggca gcggtcccga cggggccacc tcgaccgatc gcgtctgggt
    12301 cgcctcgatc gcgcgcgact tgccgcaaga gcagatcaac gccaccgcgg gcgccggcac
    12361 gatcggcgtg acgggctacg cgatcgacga cggtcaggcc tacgccgtct acgcccagga
    12421 ccagcagacc gcgggcaaga tcgagctgtt cagccgcccg ctcgacagcg acgccgacgg
    12481 cgtcgcgagc cccgacgaca actgcccgtt cgacgccaac gcgtcgcagc aaccggtcgt
    12541 cttcgctcaa accgtattcg cgaccgggac gcacggcttc ggctgggccc agccgaccga
    12601 cgcgcgctgg gtgcgcggcc cgctcgatca gctcgacgtc tatgcaacca acggctccgg
    12661 cacggcggtc ggcgccgtcg cgttcaccga tgagacgagc cccgcgccgg gcagcggttt
    12721 gtactacctg ttcgcgccgg actgcgcggg acgaagctac cagacggcgc cgggtgggga
    12781 gccggcgcgc gatctggcca cgttcccgta ggctgccgtc cgctcacagc ggaagctcga
    12841 ggccctcgtg ggcgacctcg gtcgcgctga actcctgtcg cgcgacctgt cgcagccggt
    12901 c
//
LOCUS       DASPGN010000012         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_119726, whole genome shotgun sequence.
ACCESSION   DASPGN010000012 DASPGN010000000
VERSION     DASPGN010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119726"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..2121)
                     /locus_tag="VD788_00370"
     CDS             complement(322..2121)
                     /locus_tag="VD788_00370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HXV74745.1"
                     /translation="METTVPLDETWRLEDIYADDAAFLAARRELEAVVPTLDRWRGRL
                     AGDPAVLADALDVVTDAYRRFASLRCYASLRSDRDTRIAANHARRQQLDLLAAELSSR
                     LAYLRPEILAAEPRMIEQAIVAEPRLAPHAFFLRDLMRQRQHVLGPSEERLLSHASLM
                     GGSADALYNVLYNAELPRPELRLSDGQTVRLTPVNFHVHRSAAERRDRLRIFPAYFGA
                     YASFQDTLGQNLFNAVKAHLFRVRSRGYDSCLAAALDGNNIPVVVYRNLIEQVRRRFH
                     VMHDYFRLRAEALGLERLEYPDIYCPITRSRPPRFTSTEARRVVRESLAPLGARYTAA
                     VDRAFGERWLDWHPAPGKRSGAYATGWAYDVHPYVLLNFTGDYESVSTLTHEMGHAIH
                     SHMSNAAQPFATADYPIFVAEVASTLNETLLSRHLFDRAADRDERLFLLGSQLDGFRG
                     TLFRQTMFAEFELAIHEQVERGEVLTGESLNATYLALLRDYHGHDDGVVHIGDEYALE
                     WATVPHFYYDFYVFQYATGIVAASALADAVLAQGAPAVERYLAFLAAGGSDYALDLLR
                     EAGVDLESADPYDATFATIERQVGHARALLGDR"
     gene            2517..2699
                     /locus_tag="VD788_00375"
     CDS             2517..2699
                     /locus_tag="VD788_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74746.1"
                     /translation="MLYGGRVVVGSASAPTVVVDGDGRGPYLARLSVADGHGGEDLSY
                     VVVPAERRARGTGGGS"
     gene            complement(2678..2763)
                     /locus_tag="VD788_00380"
     tRNA            complement(2678..2763)
                     /locus_tag="VD788_00380"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2728..2730),aa:Leu,seq:caa)
     gene            2964..3509
                     /locus_tag="VD788_00385"
     CDS             2964..3509
                     /locus_tag="VD788_00385"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXV74747.1"
                     /translation="MPYRARKEQLLNFKVGDKVVYPNHGVGVIEQVARRSIGETDASF
                     YRLRIVATDSTVMVPVENTETVGLRKVLSRRETSRVLKALKEGDVTTYDDWKGRFQAN
                     SEKMRTGDVRAVAEVLKSLTMLNEVKPLSYRERKMLDRARFLLVSELAEASGKGADEV
                     ESQIDTALDECLKGAQAKTEH"
BASE COUNT          622 a         1160 c         1312 g          527 t
ORIGIN      
        1 agctgagcag catctggacc cacagcacga agtcgaaggc gccgaccccg gcgagccggt
       61 agcaaccggc gagcacgatc ggaaacagcg gcgcctgatg gaagaccgcc tcgtcgccga
      121 gaccaccggt cgcgacgcgc gcggcccagt cgtggtagaa cagggcgtcc gccacgagcc
      181 cgtcgtgcag cggatcgtcg cggtactgta gccaaacgac cgcatgaacg gcgagcgccg
      241 cgacgaaaac gagcgccgac ggggttgcgg ccgaacgggg tttcgaccca cgtccgccgg
      301 caccgcctcg cttggcgcga gtcaccggtc gccgagcagc gcccgcgcgt ggccgacttg
      361 tctctcgatc gtcgcgaacg tcgcgtcgta tggatcggcc gactcgaggt cgactccggc
      421 ctcacgcagc aggtcgagcg cgtagtccga tccgccggcg gcgagaaaag cgagatagcg
      481 ctcgacggcc ggcgcccctt gcgcgagtac cgcgtcggcc agcgcgctcg cggcgacgat
      541 cccggtcgcg tattggaaga cgtagaagtc gtagtagaag tgcgggaccg tcgcccactc
      601 gagggcgtat tcatcgccga tgtggacgac gccgtcgtcg tggccgtgat agtcgcgcag
      661 cagtgcgagg taggtcgcat tgagcgactc gccggtcaag acctcgccgc gttcgacctg
      721 ctcgtggatc gccagctcga actcggcgaa catcgtctgg cggaacaacg tcccgcggaa
      781 cccgtcgagc tggctgccca agaggaacag ccgctcgtcg cgatccgccg cgcggtcgaa
      841 caggtgtcgc gacaacagcg tctcgttcaa cgtcgacgcc acctcggcga cgaagatcgg
      901 gtagtcggcg gtcgcgaacg gttgcgccgc gttactcatg tgggaatgga tggcgtggcc
      961 catctcgtgg gtcaacgtcg agacgctctc gtagtcgccg gtgaagttga gcaggacgta
     1021 ggggtggacg tcgtaggccc agccggtcgc gtaggcgccc gagcgttttc cgggcgccgg
     1081 gtgccagtcg agccaacgct cgccgaacgc gcgatcgacg gcggccgtat agcgtgcccc
     1141 gagcggggcg aggctttcgc gcacgacgcg gcgcgcctcg gtgctggtga agcgcggcgg
     1201 gcgcgaccgc gtgatcggac agtagatgtc gggatactcg agccgctcga ggccgagcgc
     1261 ctcggcccgc aagcggaagt agtcgtgcat cacgtggaat cgtcgacgga cctgctcgat
     1321 cagattgcgg tagacgacga ccgggatgtt gttcccgtcg agcgccgcgg cgaggcacga
     1381 gtcgtagccc cggctgcgga cgcggaacag atgtgccttg accgcgttga acagattctg
     1441 accgagcgtg tcctggaacg aagcgtacgc gccgaagtac gcggggaaaa tgcgcagccg
     1501 gtcgcgccgc tcggcggccg agcggtggac gtggaagttg accggcgtca gccggacggt
     1561 ctgtccgtcc gacagtcgca gctcgggccg gggaagctcg gcgttgtaaa gcacgttgta
     1621 gagcgcatcg gccgagccgc ccatcaggct ggcgtgagac agcaggcgct cctcggacgg
     1681 accgagcacg tgctggcgct ggcgcatcag atcgcggagg aagaaggcgt gcggcgcgag
     1741 gcgcggctcc gcgacgatcg cctgctcgat catccgcggc tccgcggcga ggatctcggg
     1801 acgcagatag gcgagccgac tgctcagttc ggccgcgagc aggtcgagct gctgacgcct
     1861 cgcgtggttc gccgcaatgc gcgtgtcgcg gtccgaacgc agcgacgcgt agcaacgcag
     1921 ggaggcgaag cggcgatagg cgtccgtcac cacgtcgagc gcgtcggcca acaccgccgg
     1981 atccccggcc agccggccgc gccagcgatc gagcgtcggg acgaccgcct cgagctcgcg
     2041 gcgcgcggcg aggaacgccg cgtcgtcggc gtagatgtcc tcgagcctcc acgtctcgtc
     2101 gagcggtacg gtcgtctcca ccggtctctc cggccgtgcc ccacctcgtc gcggacctac
     2161 cacgcgaggc gggagcgggg gagtggcgac gataaggcac tccccggcgg tgggcaagtc
     2221 ggcgcaacac ccgcccgtga cgtacgtaga accggggcga gggaggagcg gccacgcacc
     2281 cgagggagat caccccggca cggatcgcct cgatcgcggt cgcgggcgtc tgtctcgcgt
     2341 tttcggccgg tccccgaacg aacggttccg gcggaatcgg ggcggccgag ggcgccgagc
     2401 cgggcaaccg ctccccgctg gccgtcatcc gcgtcacaca cggcgcgggg gagcggacga
     2461 cgctcgccgc gacggggtcg ggcgatccgg acggggacgc gctgcgctac gagtgggtgc
     2521 tgtacggcgg ccgggtcgtc gtcggatcgg cgtcggcacc cacggtcgtc gtggacggcg
     2581 acggccgcgg cccgtacctg gcccggctga gcgtggccga cggacacggg ggcgaggact
     2641 tgtcctacgt cgtggtcccg gccgagcggc gggcgcgtgg taccggaggt gggagttgaa
     2701 cccacacgag gttgccctcg ggggattttg agtcccctgc gtctgccatt ccgccactcc
     2761 ggcgtcgaga cccgatctta tcgcacccac gcgcgacgat ccaagtcgcg tgcctcgcgt
     2821 cccgcggcga tcgcaacgaa tcggacgcag tcgtggcgag ggtcggccgg agacggtggc
     2881 ggatccggtt aagtagttga cacagcttgc aattatcgat accattaaga cggccaggag
     2941 tagattccgg ccctctgtcc tccatgccat accgcgctcg aaaggaacag ctcttgaatt
     3001 tcaaagtcgg agacaaggtc gtctatccca atcacggtgt cggggtgatc gaacaggtgg
     3061 cgcgccgctc gatcggcgaa acggatgcgt cgttctaccg gttgcgcatc gtcgcgaccg
     3121 acagcacggt gatggtgccg gtcgaaaata ccgagacggt agggctccgc aaagtgctgt
     3181 cgcggcgcga gacgagtcgc gtgctcaagg cgctcaagga aggtgacgtt accacctacg
     3241 acgactggaa gggtcgattt caggcgaatt ccgagaaaat gcggacgggc gacgtcaggg
     3301 cggtcgcaga agtgctcaag agcctcacga tgctcaacga ggtcaagccg ctgtcgtacc
     3361 gtgagcgcaa gatgctcgac cgggcgcggt tcctgctcgt gtcggagttg gccgaggcga
     3421 gcggcaaggg cgccgacgag gtcgaatcgc agatcgacac ggcactcgac gaatgcctga
     3481 agggggcgca ggccaagacc gagcactgac ccgaggaacc gtcagattct tcgatcctac
     3541 cgacccggcc cgcacggccg ggtcgtttcg tttcggcgtc cgggcggagg cgcgagcttg
     3601 gacaacctgg tgctgatccg g
//
LOCUS       DASPGN010000013        11649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_236987, whole genome shotgun sequence.
ACCESSION   DASPGN010000013 DASPGN010000000
VERSION     DASPGN010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11649
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236987"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VD788_00390"
     CDS             complement(<1..896)
                     /locus_tag="VD788_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74748.1"
                     /translation="MMPGWPPGRREIALVLACAALASCGTGGPAVEPGPVRLTGDWLG
                     QPAPGDRAARFAPGIVSTGMYTRDLSATPDGDEVYFTVMLPAFEFSTILFVRRDADGV
                     WGEPQVAPFSGSYRDLEPFVTPDGSKLLFVSFRPDDQRGEPSSESDVWVVDRSGDGWG
                     DPRPLGVPVNTDGEEYFPSTTRDGTLYFTRRRADGDEAIYRAEWRDERYAEPERLGPE
                     VNAGRARFNAFVSPDERFLIVPIFGLDDSLGGTDYYVNFRTPDGHWTPPFHLGAAVNS
                     ESRLEYSASLSPDGRYLFFMSAR"
     gene            complement(893..2314)
                     /gene="lpdA"
                     /locus_tag="VD788_00395"
     CDS             complement(893..2314)
                     /gene="lpdA"
                     /locus_tag="VD788_00395"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXV74749.1"
                     /translation="MADGAQAELVVVGAGPGGYAAAFLAADLGLDVTLIDPEPNPGGV
                     CLYRGCIPSKALLHVAKLIGEARQAASWGVTFGEPRIDLERLRGFKQGVVERLTRGLG
                     ELARKRKVEHLRGRARFTAPDRLTVEANDGRRSALGFRHAIIATGSRPVQPAGFPENT
                     ELLLDSTGALDLPDVPRRLLVVGGGYIGLELGSVYAALGSEVSLVEMTDGLLPGVDRD
                     LVRPLANRLGRELSSIRLATKVVEMTPDGDGLRVRLESGDGESVEQRFDRALVSVGRR
                     PNSEQLGLEQTAVVVGESGFIQVDEQLRTAEPTIFAIGDVVGQPMLAHKASHEGRTAV
                     EAIAGHKVAFEPAAIPAVVFTDPEVAWCGLTEDQAKAEGRKVSIARFPWGASGRALTL
                     DRDDGLSKLVIDGESERILGVGLVGAGAGELIAEGVLAVEMGAVAADVKLTIHPHPTL
                     SETMMEAAEVFYGQSTHVYRPKR"
     gene            complement(2317..3645)
                     /locus_tag="VD788_00400"
     CDS             complement(2317..3645)
                     /locus_tag="VD788_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HXV74750.1"
                     /translation="MEEVRLPELGENIEVVEVAAVLVAVGDRVNKDQPLVEVETEKAN
                     LEVPAPAAGTVAELLVRAGDEIRVGQVILKLGNALDPDPAPGAEDAEPVAEAREEPAP
                     ADDAEQPPASDDRAAEAPAAESVIEFPKRDAGRKREIGPTVPAAPSVRRLARELGLDI
                     QQVSGSGPAGRISRDDVKAHAKQLLLGLASGAAPAVAVAATRLPDFRQWGEIATEPLS
                     NVRRATAHNMAAAWATVPHVTQFDRADVTQLESMRKRFNEREEAAGRKLTMTAIVIKT
                     VAAALRRYPKFNSSLDLMNDVIVYKKYCHVGVAVDTERGLLVPVIRDVDRKSVVQVSG
                     ELSDLARRARDKKLAPDEMAGAGFTVSNLGGLGTSYFSPIVNWPQVAILGVGRAEQQA
                     VFDQGEFRPHLMMPLSISYDHRVIDGAEAARFLRWLAEALEQPLLLLLDE"
     gene            complement(3666..6347)
                     /gene="aceE"
                     /locus_tag="VD788_00405"
     CDS             complement(3666..6347)
                     /gene="aceE"
                     /locus_tag="VD788_00405"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HXV74751.1"
                     /translation="MAQTLKDEAQREFEAIEEQEWLESLDDVIRRGGESRVRELLRAL
                     QRRAAEFGISLPFSANTPYLNTIARGDQPRFPGHREIERRIKSLIRWNAMAMVVRANR
                     DESGIGGHISTYASAATLYEVAFNHFFKGRDHEQGGDQIFFQGHASPGIYARAFLEGR
                     LTRQQLENFRRELKPGGGLPSYPHPRALPEFWEFPTVSMGLGPIMAIYQARFNRYLED
                     RGLIERTGRVWAFLGDGETDEPESLGAISLAVRAKLDNLIFVINCNLQRLDGPVRGNG
                     KIIQELEAVFRGAGWNVIKVIWGDSWDPLLKRDDDGLLAKRMEQVVDGDYQNYSVKPG
                     SFIREHFFGSHPKLQALVEHLTDEELAKLRRGGHDPEKVYAAYRAAVEHRDAPTVVLA
                     KTVKGYGLGEAGEGKNITHQQKKLNLEELREFRSRFGIPISDDDVGEAPFYRPSDDSA
                     EIRYLRERRMQLGGYVPARSHDTPPIDPPAAEVFDEFYGGSDEREASTTMVMVRLLAK
                     LLKDENVGRLIVPIVPDEARTFGMEALFRQVGIYSHVGQRYEPVDRETLLYYKEATDG
                     QILEEGITEAGSISSFIAAGTAYATHGVNTIPLFIFYSMFGFQRVGDLVWAAGDMGCR
                     GFLVGGTAGRTTLAGEGLQHQDGHSHVLAYTHPHVVAYDPAFAYELAVIVDEGIRRMY
                     VERENVCYDLTVENEPYVMPPMPEGVRDGIIRGAYRFRVAGKKTQAKQVRLLGSGAIL
                     NEVLEAQRILDERFGVASEAWSVTSYQQLLRQALEVARWNRLHPASTPRQAYVSRCFG
                     ASNDGLVVAASDYLKALPYSLRSWIGGDFVGLGTDGFGRSERRSALREYFEVNARHIA
                     WAVIESRVRTGALDAQMLERAKHELEIDTDKPDPLAL"
     gene            6574..8934
                     /locus_tag="VD788_00410"
     CDS             6574..8934
                     /locus_tag="VD788_00410"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HXV74752.1"
                     /translation="MLDVRIARAGSAFASLALVSAGAALPCVAETVFPIGRRDRPVVA
                     VPRLDRAPTLDEFVEMRPPADLAEHLAHITGFLQRQPDDGRPASQRTDVYLGHDDEVL
                     YVVFVAWDDDPRKIRAHLSRREQVFADETVEIQLDTFDDEQRAFSFLTNPFGIQWDAI
                     WTEGQGFDESWDTVWQSKGVLTDRGYVVWMAIPFKSLRFRPRPEQEEHTWGVVLVRDI
                     PRNNETSFWPQVTSTIEGRLNQAATARGFAGVAPGRNLWLIPYAAGAREDFTPQPDAP
                     TSEFEERDVGLDAKWVVRDSLTLDATINPNFAQIESDDAQVTINRRFEVFFPERRPFF
                     LENADYFRTPLDLLFTRRIVDPEYGARLTGQLGAYKLGAFVIDDEFPGRLAAAPAALA
                     GERAINGIFRLRRDLPNQSNVGLLLTARELDRSYNRVGGVDSRVKLGSNWDTQLQAVF
                     SSSRLQDFTEPTTPDAQLDDSAWSASFNRSGRKLNAHLHYLDIGPEFRTELGFIPRTD
                     VRDAHAEVSYNFWSEDKALIRVQPLLFVQRIDNHDGVRLDESVSPVISWEFRRRTTVG
                     LFGRAARERLLKCRDYFDPTCDSALPPPGSVVLPADLDFDVGEVGLSFDTAFVAAVEL
                     DLNYEIGRGINLRPVAETAPAPADVRELAAELTLRLGRRWRIVTEWLRSELDDRASGR
                     RIFFNEILRSRFDWQLNPRLSLRAITRYDRTEVEPSLTAIRPENALNGDLLATYLVNP
                     WTAFYVGYNTNRTQTGLDSDGNPVLLDERFTNSNLAFVKVSYLFRP"
     gene            complement(8916..9647)
                     /locus_tag="VD788_00415"
     CDS             complement(8916..9647)
                     /locus_tag="VD788_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXV74753.1"
                     /translation="MRWLVGDVHGCARELDRLLREVRFDPSRDELWSVGDLVNTGPDS
                     LAALRLWRELGGRGVLGNHDIYALRVAAGMRARRPDTLDELLGADDHQLLLDALRALP
                     VLVRLSGDERGPPVWLVHAGLHPGWSDLAAVARRINRLPHDDGWTTSPWVSFAVHVRC
                     CTADGEMSEDPGPPTACAEPFRPWDSFYTGDSLVVHGHWALRGHYRGPRTMSLDSGCV
                     YGGGLTAWCQEEDRLVRVPSYGRKR"
     gene            complement(9650..10600)
                     /locus_tag="VD788_00420"
     CDS             complement(9650..10600)
                     /locus_tag="VD788_00420"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXV74754.1"
                     /translation="MAAGSDAQFPQETGERGEFIRQRSAFRQWVTADPGADHPAEPGR
                     YHLYVSYACPWAHRTIIFRRLKRLERVVSMTVVDPVRDERGWAFTAGPGCGPDPINGF
                     RFLAEAYRATDPTFHARPSVPVLWDKKTGRIVSNESSEIIRMFNREFDEWADRDVDFY
                     PEAWRGEIDGINDVVYTHVNNGVYRCGFATTQAAYDEAFEALFTTLDDLERRLAHRRY
                     LVGDRLTEADWRLFTTLIRFDPVYVGHFKCNLRRLVDYPALWAFTRELYQHEQIASTV
                     DFDHIKRHYYVTHRRLNPSGIVPNGPRIDYDEPHGRDRLG"
     gene            complement(10683..>11649)
                     /locus_tag="VD788_00425"
     CDS             complement(10683..>11649)
                     /locus_tag="VD788_00425"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74755.1"
                     /translation="VVWLGLKHRNPLVFTAANPAIEAGGFIAESKYRILQSLRGAGSA
                     VARARLLPERDSPAARLEAAKRFVEEQALDWPVVLKPDAGQRGSGVAVVRDEQALASY
                     LAAADFDVLVQEYVPGHELGVFYYRYPGEPHGRIFSITDKRMPYVTGNGRDTLERLIL
                     LDGRAVCMAEHYLAARADQLFCVPAEGERVRLVELGTHCRGAVFLDGRRFITPDLERE
                     IERISRTFDGFFFGRYDLRGDLGEIERGRGFKVIELNGVTSEATHIYDPARSLFGAYA
                     ILFRQWSIAFEIGRRNHDSGVPVASLRLLIDSIKRYRRLSRSHPG"
BASE COUNT         1814 a         4120 c         3897 g         1818 t
ORIGIN      
        1 cgcgcgctca tgaagaacag atagcgcccg tccggactca gcgaggccga gtactcgagg
       61 cgcgattccg agttgaccgc ggcacccaga tggaaaggcg gggtccagtg cccgtcgggg
      121 gtgcggaagt tgacgtagta gtccgtgcca cccaggctgt cgtccaggcc gaagatcggc
      181 acgatcagga atcgctcgtc cggcgagacg aaggcgttga accgcgcgcg gccggcgttc
      241 acctcggggc cgagccgctc cggctcggcg tagcgctcgt cgcgccactc cgcacgatag
      301 atcgcctcgt cgccgtcggc gcggcggcgg gtgaagtaca gcgttccgtc gcgcgtcgtc
      361 gacgggaaat actcttcgcc gtcggtgttg accggcacgc cgagcgggcg tgggtcgccc
      421 caaccgtcac cgctgcggtc gaccacccag acgtcgctct cgctcgacgg ttcgccgcgt
      481 tgatcgtccg ggcgaaacga aacgaacagc agcttcgagc cgtccggcgt gacgaacggc
      541 tcgagatcgc gatagctgcc cgagaacggg gcgacctgcg gttcgcccca caccccgtcg
      601 gcgtcgcggc ggacgaagag aatcgtcgag aactcgaagg ccgggagcat gacggtgaaa
      661 tagacctcgt cgccgtcggg ggtcgcgctc agatcccgcg tgtacatccc ggtcgagacg
      721 atacccggcg cgaaacgtgc ggcacggtca ccgggcgcgg gctgaccgag ccagtcgccg
      781 gtcaaccgaa cggggccggg ctcgaccgcc gggccgcccg tgccgcagct ggcgagtgcc
      841 gcacaggcga ggacgagcgc gatctcgcgc cggcccggcg gccaccccgg catcatcgct
      901 tcggccggta gacgtgagtg ctctgaccgt agaacacctc ggccgcttcc atcatcgtct
      961 cggacagcgt cgggtgcggg tggatcgtca gcttgacatc ggccgcgacg gcacccatct
     1021 cgaccgccag cacgccttcg gcgatcagct cgccggcgcc cgcgccgacg agaccgacgc
     1081 cgaggatccg ctcgctctcg ccgtcgatca ccagtttcga cagaccgtca tcccgatcga
     1141 gcgtgagcgc gcggcccgac gcgccccacg ggaagcgcgc gatcgagacc ttgcgaccct
     1201 cggccttggc ctgatcctcg gtcagcccgc accaggcgac ctcgggatcg gtgaatacca
     1261 cggccgggat cgccgccggc tcgaacgcga ccttgtgtcc ggcgatcgcc tcgacggccg
     1321 tccggccctc gtgcgacgcc ttgtgggcca acatcggttg gccgacgacg tcgccgatgg
     1381 cgaagatcgt cggttcggcg gtgcgcagct gctcgtcgac ctggatgaag ccggactcgc
     1441 cgacgaccac cgcggtctgc tcgagaccga gctgttcgga attcggccgc cggccgacgg
     1501 agaccagcgc gcgatcgaag cgctgttcga ccgattcgcc gtcgccgctc tcgagtcgca
     1561 cgcgcaagcc gtcgccgtcg ggcgtcatct cgacgacctt cgtcgcgagg cggatcgagc
     1621 tcagctcgcg cccgagcctg ttcgcgagcg ggcggaccag gtcccgatcg acgccgggca
     1681 gcagcccgtc cgtcatctcg accaggctca cctcgctccc cagcgccgcg tagaccgaac
     1741 ccagctcgag accgatgtat ccgccgccga cgaccaacag ccggcgcggt acgtccggca
     1801 ggtcgagcgc accggtcgaa tcgagcagca gctcggtgtt ctcgggaaaa ccggccggct
     1861 ggaccggccg cgaaccggtg gcgatgatcg cgtggcggaa gccgagcgcg ctgcgccggc
     1921 cgtcgttcgc ttcgacggtg agccggtcgg gcgcggtgaa ccgcgcgcgg ccgcggagat
     1981 gctctacctt gcgcttgcgg gccagctcgc ccagcccgcg ggtcagccgc tcgacgacgc
     2041 cctgcttgaa gccgcgcagg cgctcgaggt cgatccgcgg ctcgccgaat gtcacgcccc
     2101 aactcgccgc ctgacgcgcc tcgccgatca gcttggccac gtgcagcagc gccttggacg
     2161 ggatgcagcc gcggtagaga cagaccccgc cggggttcgg ctcgggatcg atcagcgtca
     2221 cgtcgagacc cagatcggcg gcgaggaacg cggcggcgta ccctccgggc ccggccccga
     2281 cgaccaccag ttcggcctga gcgccgtcgg ccatcgtcat tcgtccaaca gcaacaacag
     2341 cggttgctcg agcgcctcgg ccagccagcg caggaagcgc gcggcctccg cgccgtcgat
     2401 cacccggtgg tcgtaggaga tcgacagcgg catcatcaag tgcggccgga actcgccctg
     2461 gtcgaacacc gcctgctgct cggcacggcc gacgcccagg atcgcgacct gcggccagtt
     2521 gacgatcggc gagaaatagc tcgttcccag accgccgagg ttcgacacgg tgaaaccggc
     2581 tcccgccatc tcgtcggggg cgagcttctt gtcgcgcgcg cggcgggcga ggtcgctcag
     2641 ctcgcccgac acctgcacca cgctcttccg gtcgacgtcg cggatcaccg ggacgagcaa
     2701 gccacgctcg gtatcgaccg cgacgccgac gtgacagtac tttttgtaga cgatcacgtc
     2761 gttcatcagg tcgaggctcg agttgaactt gggatagcga cgcagcgccg ccgcgaccgt
     2821 cttgatcacg atcgcggtca tcgtcagttt gcgtcccgcc gcctcctcgc gctcgttgaa
     2881 gcgcttgcgc atcgactcga gctgcgtcac gtcggcgcgg tcgaactgcg tgacgtgcgg
     2941 gacggtggcc caggccgccg ccatgttgtg ggccgtcgcg cgcctgacgt tgctcaacgg
     3001 ctcggtggca atctcgcccc attgtcggaa gtcgggcaga cgcgtcgcgg cgacggcgac
     3061 cgccggtgcc gcgcccgagg cgagaccgag cagcagctgc ttggcgtggg ccttcacgtc
     3121 gtcgcgcgaa atacgccccg ccggaccgct ccccgacact tgctggatgt cgaggccgag
     3181 ctcgcgtgcc aggcggcgga ccgagggtgc cgcggggacg gtcggcccga tctcgcgctt
     3241 gcggccggca tcgcgcttgg ggaactcgat caccgactcg gcggcaggcg cctccgcggc
     3301 tcggtcgtcg ctcgccggcg gttgctcggc atcgtcggcc ggggccggtt cctcacgggc
     3361 ctcggccacg ggctcggcgt cctccgcgcc tggcgcgggg tcggggtcga gcgcgttgcc
     3421 gagcttgagg atcacctgcc cgacgcggat ctcgtccccg gcgcgaacca acagctcggc
     3481 gaccgttccc gccgcgggcg ccggcacctc gagattggcc ttctcggtct cgacctcgac
     3541 cagcggctgg tctttgttca cccgatcgcc caccgcgacc agcacggccg ccacttcgac
     3601 gacctcgatg ttctcgccca gctcgggtag tctcacctct tccacgtcgg catcctcgat
     3661 ccgcatcaaa gcgccagcgg atccggcttg tcggtgtcga tctcgagttc gtgcttggcg
     3721 cgctccagca tctgcgcgtc gagcgcgccg gtgcgcacgc gcgactcgat gacggcccag
     3781 gcgatgtgcc gtgcattgac ctcgaagtac tcgcgcaacg ccgaccgccg ctcgctgcgg
     3841 ccgaacccgt cggtacccag accgacgaag tccccgccga tccagctacg caacgagtac
     3901 ggcagcgcct tcagataatc ggaggccgcc acgaccaagc cgtcgttgct cgcgccgaag
     3961 cagcggctga cgtaggcctg gcgcggcgtg ctcgccgggt ggagccggtt ccagcgcgcg
     4021 acctcgagcg cctggcgcag cagctgctgg tagctcgtca ccgaccaggc ctcgctggcg
     4081 acaccgaacc gctcgtcgag aatgcgctgg gcctcgagta cctcgttcaa gatcgcaccg
     4141 ctgccgagca ggcggacctg tttggcctgc gttttcttac cggcgacgcg gaagcgatag
     4201 gcgccgcgga tgatgccgtc gcgcacgccc tccggcatcg gcggcatcac gtacggctcg
     4261 ttctcgaccg tcaggtcgta gcagacgttc tcgcgctcga cgtacatccg ccggatgcct
     4321 tcgtcgacga tcacggccag ctcgtaggcg aacgccgggt cgtaggccac gacgtgcgga
     4381 tgggtgtagg ccagcacgtg cgagtgcccg tcctggtgct gcaggccctc gccggccagc
     4441 gtcgtgcgcc ccgccgtgcc gccgacgagg aatccgcgac agcccatgtc gccggcggcc
     4501 cagaccagat cgccgacgcg ttggaatccg aacatcgagt agaagatgaa cagcgggatc
     4561 gtgttgaccc cgtgggtcgc gtaggccgtg ccggcggcga tgaacgagct gatcgacccg
     4621 gcctcggtga tgccctcttc caggatctga ccgtcggtcg cttccttgta gtacagcagc
     4681 gtttcccggt cgaccggctc gtaacgctgg ccgacatgcg agtagatccc gacctggcgg
     4741 aacagcgcct ccatgccgaa ggtgcgggcc tcgtccggca cgatcggcac gatcaaccgg
     4801 ccgacgttct cgtccttcaa cagcttggcc agcagacgca ccatcaccat cgtcgtcgac
     4861 gcttcgcgtt cgtccgagcc accgtagaac tcgtcgaaca cctccgctgc gggtgggtcg
     4921 atcggcggcg tgtcgtggct gcgggccggc acgtagccac cgagctgcat ccgccgttcc
     4981 ctgagatagc ggatctccgc gctgtcgtcg gacggtcggt agaacggcgc ttcgccgacg
     5041 tcgtcgtcgg agatcgggat gccgaagcgg gaacggaact cccgcagctc ctccaggttg
     5101 agtttcttct gctgatgggt gatgttcttg ccctcgcccg cttcacccaa cccgtagccc
     5161 ttgaccgtct tggccagcac gacggtcggc gcgtcgcggt gctcgacggc ggcgcggtag
     5221 gcggcataga ccttttccgg atcgtgaccg ccgcggcgca acttggccag ctcctcgtcc
     5281 gtcaggtgct cgaccagcgc ttgcagcttg gggtgcgagc cgaagaaatg ctcgcgaatg
     5341 aacgaccccg gcttgacgct gtagttctga taatcgccgt cgacgacctg ctccatccgc
     5401 ttcgccagca ggccgtcgtc gtcgcgcttg agcagtgggt cccagctatc gccccagatc
     5461 accttgatca cgttccagcc cgcgccgcgg aagaccgcct cgagctcttg gatgatcttg
     5521 ccgttgccgc gcaccgggcc gtccaatcgc tggagattgc agttgatgac gaagatcagg
     5581 ttgtcgagct tggctcgcac ggcgaggctg atcgcgccga gcgactccgg ctcgtccgtc
     5641 tcgccgtcgc cgagaaacgc ccagacgcgg ccggtgcgct cgatcaaacc ccgatcctcg
     5701 agatagcggt tgaaccgcgc ctggtaaatc gccatgatcg gtccgaggcc catcgacacc
     5761 gtcgggaact cccagaactc gggcaacgcg cgcgggtgcg gataggacgg cagcccgcct
     5821 cccggcttca actcgcggcg gaagttctcg agctgctgcc gcgtcaacct cccttcgagg
     5881 aaggcgcgcg cgtagatccc gggcgaagcg tgaccctgga agaagatctg gtcgccgccc
     5941 tgctcgtggt cgcgcccctt gaagaagtgg ttgaacgcga cctcgtagag cgtggccgcc
     6001 gaggcgtagg tcgaaatgtg gccaccgatc ccgctctcgt cgcggttggc ccgcacgacc
     6061 atcgccattg cgttccagcg gatcaggctc ttgatccgac gctcgatctc gcggtggccg
     6121 ggaaaccgcg gctggtcgcc gcgcgcgatc gtgttgaggt agggcgtatt ggcggaaaaa
     6181 ggcaacgaga tgccgaactc cgccgcccgg cgctgcaacg cgcgcagcag ctcgcgcacg
     6241 cggctctcgc cgccgcggcg gatcacgtcg tccagcgact caagccattc ctgttcctcg
     6301 atcgcctcga actcgcgttg cgcctcgtcc ttgagggtct gtgccatcta cgcctctctc
     6361 cgcgactcca cgccgacggt tgtcaccggg ccgactccct atcctatcgg aatcgcgcgc
     6421 cgtcgtgcac ggcccggggc aggaaccccg ggctcgtgtg ccgggcccgc cgggtccgcg
     6481 cggtaaaatt gtgtaaaact gccggccgat cgccacttcc gggtcggtcc ttccccgaca
     6541 atcgagcggt ggtgcgaacg acggtgtggg tcgatgcttg acgttcggat cgcgcgagca
     6601 gggtcggcgt tcgcgagctt ggcgctcgtc tcggccggtg ccgcgctccc ctgcgtcgcg
     6661 gagaccgtct tcccgatcgg ccgccgcgat cggccggtgg tcgcggtgcc gcggctcgat
     6721 cgcgcaccga ccctcgacga gttcgtcgag atgcgcccgc cggccgatct cgccgagcat
     6781 ctcgcgcaca tcaccggctt cctgcagcgc cagccggacg acggccgacc ggccagccaa
     6841 cggaccgatg tctacctcgg ccacgacgac gaggtgctgt acgtcgtctt cgtcgcctgg
     6901 gacgacgacc cgcgcaagat ccgggcccac ctcagccgcc gcgagcaggt gttcgccgac
     6961 gaaacggtcg agatccagct cgacaccttc gacgacgagc agcgggcctt ctccttcctc
     7021 accaacccgt tcggcatcca gtgggacgcg atctggaccg agggccaggg gttcgacgag
     7081 tcgtgggaca ccgtctggca gtcgaagggc gtgctgaccg accggggcta cgtcgtctgg
     7141 atggcgattc cgttcaagag cctgcggttc cggccgcggc ccgagcaaga agaacacacc
     7201 tggggggtcg tgctggtgcg ggacatcccg cgcaacaacg aaacctcgtt ctggcctcag
     7261 gtcacgagca cgatcgaggg gcggctgaac caggcggcca ccgcacgggg gttcgccggc
     7321 gtggcgccgg ggcgcaacct ctggctgatc ccctacgccg ccggcgcccg tgaggacttc
     7381 actccgcagc ccgacgcgcc gaccagcgag ttcgaggagc gcgacgtcgg gctcgacgcc
     7441 aagtgggtcg tgcgcgacag cctgacgttg gacgcgacga tcaaccccaa cttcgcccag
     7501 atcgaatcgg acgacgcaca ggtgacgatc aaccgccggt tcgaagtgtt cttccccgag
     7561 cgccggccgt tcttcctcga gaacgcggac tacttccgca ctccgctcga cctgttgttc
     7621 acgcggcgga tcgtcgatcc ggagtacggc gcccggctga ccggccaact cggcgcctac
     7681 aagctcggcg ccttcgtcat cgatgacgag tttcccggcc gcctcgccgc cgcgcccgcc
     7741 gcactggcgg gcgagcgcgc gatcaacgga atttttcggc tgcgtcgcga cctgcccaac
     7801 cagtcgaatg tcggcctgtt gctcaccgcc cgtgagctgg accgatccta caatcgcgtc
     7861 ggcggcgtcg actcgcgcgt caagctcggc agcaactggg acacccaact acaggccgtg
     7921 ttctcctcgt cgcggctgca ggacttcacc gagccgacca cgccggacgc gcagctcgac
     7981 gacagcgcgt ggagcgcgtc gttcaaccgc agcgggcgca agctcaacgc tcatctgcac
     8041 tacctcgaca tcggccccga gttccgcacc gagctgggct tcatcccccg caccgacgtg
     8101 cgcgacgccc acgccgaagt gagctacaac ttctggtccg aggacaaggc gctgatccgc
     8161 gtgcagccgc tgctgttcgt ccagcgcatc gacaaccacg acggggtgcg gctcgacgaa
     8221 tcggtcagtc cggtgatctc gtgggagttc aggcggcgca cgaccgtcgg cctgttcggc
     8281 cgcgcggcgc gcgagcggct gctcaagtgc cgggactact tcgacccgac ctgcgacagc
     8341 gcgctccctc cgcccgggtc ggtcgtgctg ccggccgacc tcgacttcga cgtcggcgag
     8401 gtcggcctgt cgttcgacac cgctttcgtc gcggcggtcg agctggacct gaactacgag
     8461 atcggccgcg ggatcaacct gcgtccggtc gccgagaccg cgccggcgcc ggccgacgtg
     8521 cgggagctgg ccgccgagct gacgctgcgg ctcgggcgcc gctggcgcat cgtgaccgag
     8581 tggctgcgct cggagctgga cgatcgcgca tccggccgtc gcatcttctt caacgagatc
     8641 ctccgctcgc gcttcgactg gcaactgaac ccgaggttgt cgctgcgcgc gatcacacgc
     8701 tacgaccgca ccgaggtcga gccgtcgttg acggcgatcc gccccgagaa cgcgctcaac
     8761 ggcgaccttc tcgcgaccta cctggtcaac ccgtggaccg cgttctacgt cggctacaac
     8821 accaaccgca cccagaccgg gctcgactcg gacggcaacc cggtcttgct cgacgagcgg
     8881 ttcaccaact cgaacctggc cttcgtcaag gtctcctacc tctttcggcc gtaggacgga
     8941 acgcgcacga ggcggtcctc ctcctgacac cacgccgtca gcccgccgcc gtagacacag
     9001 cccgagtcga gactcatcgt gcgcggcccg cggtagtggc cgcgtagggc ccagtggccg
     9061 tggacgacca gcgaatcacc cgtatagaag ctgtcccacg ggcggaacgg ctcggcacag
     9121 gccgtcggcg ggccgggatc ttcgctcatc tcgccgtccg ccgtgcagca gcggacgtgg
     9181 accgcgaacg agacccacgg gctggtcgtc cacccgtcgt cgtgagggag tcggttgatt
     9241 cgccgggcga ccgcggcgag atcggaccag ccggggtgca gcccggcgtg aaccagccag
     9301 accggcggtc cgcgctcgtc gcccgagagc cgaacgagga ccggcagcgc ccgcagcgca
     9361 tcgagcagca gctggtggtc gtccgctccg agcagctcgt cgagcgtgtc gggccggcgg
     9421 gcgcgcatcc cggcggcgac gcgcaacgcg tagatgtcgt ggttgccgag cacgccccgg
     9481 ccgcccagct cgcgccacag ccgcagtgcg gccagcgagt cggggccggt gttgaccagg
     9541 tcgccgaccg accacagctc gtctcgcgac gggtcgaagc ggacctcgcg cagcagccgg
     9601 tcgagctcgc gcgcgcagcc gtggacgtcg ccgaccagcc agcgcacgat cagccgagcc
     9661 ggtcgcgccc gtgcggctcg tcgtagtcga tccgcggccc gttgggcacg ataccggaag
     9721 ggttcagccg gcgatgggtg acgtaatagt ggcgcttgat gtggtcgaag tcgacggtgc
     9781 tcgcgatctg ctcgtgctgg tacaactcgc gcgtgaacgc ccacaacgcc gggtagtcga
     9841 ccagtcgccg caggttgcac ttgaagtgac cgacgtagac cgggtcgaat cgaatcagcg
     9901 tggtgaacag ccgccagtcg gcctcggtca gtcgatcgcc gacgagataa cgccgatgcg
     9961 ccagccgccg ctcgaggtcg tcgagcgtgg tgaacagcgc ctcgaacgcc tcgtcgtagg
    10021 ccgcttgggt cgtggcaaac ccgcagcggt agaccccgtt gttgacgtgg gtgtagacga
    10081 cgtcgttgat cccgtcgatc tcgccgcgcc aggcctcggg atagaagtcg acgtcgcggt
    10141 cggcccactc gtcgaactcg cggttgaaca tgcggatgat ctcggacgat tcgttgctga
    10201 cgatgcgtcc ggtcttcttg tcccacagca ccggaacact cgggcgcgcg tggaacgtcg
    10261 gatcggtcgc gcggtaagcc tcggccagga agcggaagcc gttgatcggg tcggggccgc
    10321 acccgggacc ggcggtgaat gcccagccgc gctcgtcccg aaccgggtcg acgacggtca
    10381 tcgagacgac ccgctcgagc cgcttcagcc gacggaagat gatcgtgcgg tgcgcccacg
    10441 ggcaggcgta ggagacgtac aggtggtagc gccccggctc cgccggatgg tcggccccgg
    10501 ggtcggccgt gacccactgg cggaacgcgc tccgctgccg gatgaactcg ccgcgctcgc
    10561 cggtttcttg ggggaactgt gcgtcgctgc cggcggccat cgcttctcct cgcgagtcgt
    10621 tcattctagc gaatcccgcg tgcttcgcat ccggggcgct gcaccgcgtc gaacgcggcg
    10681 gatcaaccgg gatgcgagcg tgacagccgg cggtagcgct tgatgctgtc gatcagcagg
    10741 cgcaacgacg cgaccgggac acccgagtcg tggttgcgcc ggccgatctc gaacgcgatc
    10801 gaccactggc ggaacaggat cgcataggcg ccgaacaacg aacgcgcggg gtcgtagatg
    10861 tgggtcgcct cggacgtgac cccgttcagc tcgatcacct tgaacccgcg accgcgctcg
    10921 atctcgccga ggtcgccgcg caagtcgtag cgaccgaaga agaaaccgtc gaacgtccgg
    10981 ctgatccgct cgatctcgcg ctcgaggtcg ggcgtgatga accggcggcc gtcgagaaag
    11041 accgctccgc gacagtgagt ccccaactcg acgagccgga cccgttcgcc ttcggccgga
    11101 acgcaaaaca gctgatcggc ccgcgccgcg agatagtgct cggccatgca gaccgcgcgc
    11161 ccgtcgagca ggatcagacg ctcgagcgta tcgcgcccgt tgccggtgac gtacggcatt
    11221 cgcttgtcgg tgatcgagaa gatccgaccg tgcggctcgc ccgggtagcg gtagtagaac
    11281 acgccgagct cgtggcccgg aacgtactcc tgcaccagca cgtcgaagtc ggccgccgcg
    11341 aggtagctgg ccagcgcctg ttcgtcgcgc acgacggcga cgcccgaacc gcgttggccg
    11401 gcgtctggtt tgagcacgac cggccagtcc agcgcctgct cctcaacgaa gcgcttggcc
    11461 gcttcgagcc gcgccgcggg gctgtcccgc tcgggcagca gtcgcgctcg ggcgaccgcc
    11521 gaacccgcgc cgcgcagact ctgcaggatg cggtacttcg actcggcgat gaaccccccc
    11581 gcttcgatcg cgggattggc ggcggtgaag acaagcggat tgcggtgctt gagaccaagc
    11641 cagacgacg
//
LOCUS       DASPGN010000014        18617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_353925, whole genome shotgun sequence.
ACCESSION   DASPGN010000014 DASPGN010000000
VERSION     DASPGN010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 18617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18617
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353925"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2334)
                     /locus_tag="VD788_00430"
     CDS             complement(<1..2334)
                     /locus_tag="VD788_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXV74756.1"
                     /translation="MTTPSAGPPKLGHKHRILLRIERFSRQHYGVVFSLALLALVGGI
                     WLGSRLKLESDILALIPEGNRQVDTLRKALADFGSIDYLLVLLEAGEGEGPDELEDFA
                     DRFADRLVGLDELIEYVEYRFELDDDFLELFYRNAVLFLPPDRLGELAAKLTDEAIAE
                     RLSRLKLELSSPMASLTQDLVVNDPLRLMPLFLNRLEGNRGVLKVDLSDGYYLSQDAR
                     TLIMLVKPVGPSQNLEFDRRLMGAVRALEDEVRRELALEASAGEGGPHRVGVRYGGNY
                     AIALDEEALIRQDVSRNLIVSLIAVSALYWLCYRRFAALLYSSLPLLVGQALTFAVAF
                     FALRQLNASSSAFTALLMGLGTDFVIVMYARYVEERRRGRTLAEATELMIGETGLGVF
                     TGAITSAGTFYAMCTSEFQGLWDLGFLIGGGILLCAVAIVFMLPAMIKWNEGVRPRRT
                     DSLRKLHLQSFGLERLITLSVRYRRLTLVSLALITAVSGYLATRLGFDDTITVLRSER
                     SEALKVQDDVRKMFGASLSYMMAIAEGETLDEAIALTSRIDERLQPFIEDGTVASSDS
                     VLSYLPPLDEQLQVLAALREDETGAFDVERIRAALASGLAQQRFRPDAFSEFTDRLET
                     FLRPERPVRIEDLQQQGLQRLLDRYVALDDGRHRVVTYMFLTDRRWKREAPPGLVDAL
                     TAGDRDIVVTGTNILGQELRGLFAREAPRAVLLGLAVVFLLLWVDFRSIRLTAIAMMQ
                     LICGVTIMLGVMKVAGINLNYVNAFVATMILGVGIDYS"
     gene            2507..3727
                     /gene="rocD"
                     /locus_tag="VD788_00435"
     CDS             2507..3727
                     /gene="rocD"
                     /locus_tag="VD788_00435"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HXV74757.1"
                     /translation="METTRAKTEQFIRQEDKYGAHNYHPLDVVIHRAEGVWVWDVEGN
                     RYLDFLAAYSAVNQGHAHPRIRQAMIEQSGLVTLTSRAFRNDRLGPLYEQVSRMTGYH
                     RMLPMNTGAEAVETAIKAARKWGYKVKGVAAGKAEILVFTGNFHGRTTTIVGFSDDAQ
                     YREGFGPFTPGFTLLPYGDLAAARQAMNPSVVGVLVEPIQGESGIVIPPAGYLEGLEQ
                     ACREHNALLICDEIQSGLGRTGKLFAFEHEGVRPDIVIIGKALAGGFYPVSAILADDE
                     VMGVFHPGDHGSTFGGNPLGCAIAGAALDVLVEEKMIENSAKLGAYFLDKLRSIESRN
                     IEEIRGRGLWIGLQLTKQAGGARRYCEALEREGLLCKETHVDTIRFAPPLSITRDEID
                     WALERIRKVFGELG"
     gene            3727..4569
                     /locus_tag="VD788_00440"
     CDS             3727..4569
                     /locus_tag="VD788_00440"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV74758.1"
                     /translation="MAGQASTVDIEGVALHLAHPDEIAIEWVGQHETMRQLLAAWSVI
                     DQRDVPMSPRLLGKPGVGKTTLAYAAAKRLGRDAYIFQATMDTRPEDLLITPVVDKQG
                     SIRYVASSLVTAMIRGDVAILDEGNRMSEKSWASLAPLLDNRRYVESIVAGVKVAAHA
                     EFRIVATMNDDASTYELPEYIHSRLQPQIHLDFPDGDEEKRILQANLPFSDDRLLDYV
                     TRFLQAAHAADETYTVRDGVNVARYALKLIDGRAASDAACRSALAEAIRRILDDDALR
                     HFDR"
     gene            complement(4611..5855)
                     /locus_tag="VD788_00445"
     CDS             complement(4611..5855)
                     /locus_tag="VD788_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74759.1"
                     /translation="MSMSRRERSLVHALMLILFAAAPMIAMTGSVAADTPSSPPVKDC
                     GAKPEDSAADPDVYSRTLCRQDAVVAQLEHLVDVNLTGSPLASSPGMTAQRRDLAARI
                     KQEARMLHGKLRVDDFETAAKGVAKYRKAEGCEWVPLVDDNDNGICDWPDEQCQALNY
                     PEKSCDPRAKKDNPNNERYICAENCATWALRVSDEQEAEHVAQDLERTYEEIEKSVLE
                     VNDRLAFVNALLVEDASAVDRSAESCDDIYALPDGLVEAMYFLRGAHAGLAMVADIGA
                     ALNNQTVVVVAVAAGFGGGGGGNGSALSAILAGAEGLADIAHKAVQAEVETRTSLVQD
                     RTFACLQETIASLQVVNGKLDQTATELADKLDRNRQEILASQAALEARIEQARAELAV
                     RLDQITVLLSTPLGQRPGYPQP"
     gene            6096..7457
                     /locus_tag="VD788_00450"
     CDS             6096..7457
                     /locus_tag="VD788_00450"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXV74760.1"
                     /translation="MTRQVLLTTVRGALAAISLTMACAPADDPPVAETMPPADIVADG
                     PLAARIDAYAAPLAARGEFSGVVLVGSRDAILFARAYGMSDYTSDAPNRLETGFRVAS
                     ITKTFTSAAVLRLRDEGKLELDDRLSRFAPDFPRGDEITIRHLLLHRSGLRNPDYLEA
                     FERSIGLDELVDRIAARGLAFDPGTDGAYSNAGYNVLAHVVERASGTTYEDYLRQTIL
                     APLGMETTDHDGSPARGYLPAPPPVIRVVPPEADPVGFMIGSGSLVSTAPDLWRWARA
                     VADERIVGWKDLEWPYGWGRVEIGEHEGIEQTGATTGFMSSLLVFPDGDRFVVVLHNV
                     EYGPWTSFGKDIAAIAFGEERAPPPAREPYALDPGSLEQYAGRYSEADTTLTVRAEEG
                     QLRMYYNDWPIGKYLVPTSASRFAPLGDGGEIVFAGERDGVFSELSWRFGDSGTTYRR
                     GDR"
     gene            complement(7474..8991)
                     /locus_tag="VD788_00455"
     CDS             complement(7474..8991)
                     /locus_tag="VD788_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74761.1"
                     /translation="MRSSVRSVAITLTLLLPGALPPDDKSPPAEPVAVVTTHQLRIDG
                     KSIPYTATAGWLILRDDSGQPTARFGYTAYAVKDVSEPSLRPVLFAFNGGPGSSSIWL
                     HMGILGPRRVVVADPGFTPPPPVGIVDNAHTVLDVTDIVMIDPVGTGYSKVLGQGKIE
                     DHWGVDQDVRSVSRFIVRWVEQNARWRSPKYILGESYGGIRAAGLAYHLQDTHRMSIN
                     GVVLVSPFMHFGFGADGIGLDLPHALYLPGLAAAAWYHERLDDAAPVLREFLDEVERF
                     AIDEYLPALLRGYTLPLEEKREIAARAAAFTGTSTDYWLKADLRVSHGQFLQELGRDR
                     GTTTGRIDARFTGPALNPLAESQDYDPFFPAIGPAFTAGFKDYYRRELEFAVEDEYVV
                     MTRAWREWDWAHAQPGSDGVFAPPFPNVLPDLAHAMKMNPGLGVLVQQGYYDLATPYF
                     IADYYVRHLDIPDEARQRVRLAYYEAGHMMYLHPPSMAQYREDLVRFVRETDRVD"
     gene            9032..10012
                     /locus_tag="VD788_00460"
     CDS             9032..10012
                     /locus_tag="VD788_00460"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXV74762.1"
                     /translation="MVTDVGGMEFARTIKLAPRTPARLGGVMRAETEIDYAERITRVL
                     LHIQGNLDAPLDLERLAAIAHFSPFHFHRVFSGMVGESVKRHVRRLRLERAAMRLKHA
                     RQPVTRIALEAGYQSHEAFTRAFRSLFGLSPTQFRAATDPVARAGAQARVRYAPDGSV
                     AGFASIGPNDVPVDVEIRELAPMRAAFIRHVGPYDEVGSTWEQLIDWVGRECSFGPRL
                     RFFGACWDDPEVTPARSLRYDACVTVDDDVTAAGAVGVQRVAGGRFAVALHEGPYRGL
                     AASYAKLCGLWFPASGERLGDPPSLEFYLDDPESTPPEELLTEVWMRIAD"
     gene            10028..10648
                     /locus_tag="VD788_00465"
     CDS             10028..10648
                     /locus_tag="VD788_00465"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HXV74763.1"
                     /translation="MASETPEKIDLFKLHKDDYRASGTPELVFVGTANYLVVRGHGEP
                     GGECFESRVQALYGMAYTLKFTGKAAGHDFVVGKLEGLYGVDGQSIDDLARLAPQRWN
                     WRLMIRVPDRIRPQHVTEARRTLDEKGKAGDFDAVTLESIDEGRCVQMLHVGPYEQES
                     RTVAAMRELARRNGLAAHTWHHEIYLSDPRRVPAAKLRTILREPVR"
     gene            complement(10694..10888)
                     /locus_tag="VD788_00470"
                     /pseudo
     CDS             complement(10694..10888)
                     /locus_tag="VD788_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            complement(10871..13810)
                     /locus_tag="VD788_00475"
                     /pseudo
     CDS             complement(10871..13810)
                     /locus_tag="VD788_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
     gene            complement(13851..16025)
                     /locus_tag="VD788_00480"
     CDS             complement(13851..16025)
                     /locus_tag="VD788_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV74764.1"
                     /translation="MNAQPDLIGKTLARFKILDRLGSGGMGEVYLAEDATLDRRVALK
                     VLPAALAGDPNGLRRFEREAKTIAALNHPNIVTIHSVEESEGLHFLTMELVEGRSLEQ
                     RVDPGGMALPDFLRIARPLVDAVCAAHEKGITHRDLKPANVMIGDDGRVKVLDFGLAK
                     LHDGPRADDITGIQTQGLTQDGMIVGTVPYMSPEQVQGRAADHRSDIFSLGVIFFELI
                     TGKRPFRGASSADLISSILRDTPEPVSDVRVGLPNHLGRIIRRCLNKDPQDRYQTARD
                     LLNELKELRDERVSVDPDVHSIAVLPFANMSPDPAQEYFCEGIAEELINGLGRIKNLR
                     VASRTSAFQFKGSKHDIREIGDRLNVGKVVEGSVRKAGDRVRINAQLINVSDGYRLWS
                     ERYDRKLEDIFAIQDEIAENIVRALEVTLSPKERRAIQNVATRDVRAYDFYLRGRKYF
                     YEFDRRNWELARQMFERAIELDPSYALAYAGIADCWSFLFMYSGSSERERQFAEQASA
                     KALELDPDLAEAHASAGMVLSLADKHDEAGRHFERAIQLNAKLYDAYYFYARASVGQG
                     NFAKAAELYEKAIDVRPDDYQARLLLPQVYIRLERRADANQAQLAGIGAARRHLELNP
                     DDVRALYLCGGALVEVGEVDAGLELVDRAVTIAPNETGLLYNVACIYATIERTKEALD
                     HLERAVAAGYNHKAWLENDSDFDSVRDHPRFRAILNLIDVRR"
     gene            16211..17431
                     /locus_tag="VD788_00485"
     CDS             16211..17431
                     /locus_tag="VD788_00485"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXV74765.1"
                     /translation="MANVLVIQPGFPLEIPYYVRGLARAGARVLGVGDQPAESLPAPA
                     RESLAAYLQVGNLWDAAGLIDALRRWDVPVGFDRVEALWEPAMEIAAEIRRAFGLPGL
                     DAEHTTWFRDKHRMRQRLEQAGVRNIRFARVRGADEVPEAAERVGFPLIVKPVAGAGA
                     SHTYRVDGRDELIRLLPALGSVGELAVEEFVDGTEFTFDTLCAGGRILFHNILRYTPT
                     MLESRSNEWISPQNMVLRDLDRPEFRRAFRLGEQVLRALEFRSGITHMEWFLRPDGEA
                     VFCEIAARPPGGMTGELMNYCCDFDVYRALGSTILHGTIDQPIERKYNVAMVFKRAIG
                     QGRIRKIEGLAELYRRFGPHVVRHELLPLGARRRDWKQTLISDGFVILRHPDLAQAER
                     MSRFVAENVRLYAG"
     gene            complement(17435..17974)
                     /locus_tag="VD788_00490"
     CDS             complement(17435..17974)
                     /locus_tag="VD788_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV74766.1"
                     /translation="MLADELRVRILEALCDDEPRTTKQVAKRLGEKPTRLYHHVDALE
                     RVGLIRLAETRQVRGTVEKYYRAVAKVFRADSSLFRTDDAGEAAKQVVGVVQTMMLNT
                     AEDLRRLIDAGHDVAGSDEALLSYTEVHASEEQIRRLREKLMAVLHDVGETGCDEESG
                     GPGKPRRFRLTLAFFPLDL"
     gene            18171..>18617
                     /locus_tag="VD788_00495"
     CDS             18171..>18617
                     /locus_tag="VD788_00495"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV74767.1"
                     /translation="MKVDCSERRLAPFFVVWSGQAVSLIGTQAVQFALVWWLTVRTGS
                     ATVLATATFVALAPQIVLGPFVGALVDRWNRKWIMLASDGAMAAASALLALSFLDGSA
                     TSGQVFALLFVRALGAVFHGTAMTAATSMMVPVTQLTRIQGLNQMLQ"
BASE COUNT         2903 a         6259 c         6353 g         3102 t
ORIGIN      
        1 gctgtagtcg atgccgacgc cgaggatcat cgtggcgacg aaggcgttga cgtagttcag
       61 gttgatgccc gcgaccttca tcacacccag catgatcgtc acgccgcaga tcagctgcat
      121 catcgcgatg gcggtcaagc ggatgctgcg gaaatcgacc cacaacaaca ggaagacgac
      181 cgccagaccg agcagcaccg cccgcggtgc ctcgcgcgcg aacagcccgc gcagttcctg
      241 acccagaatg ttggtgccgg tgacgacgat gtcgcggtca ccggcggtca gcgcgtcgac
      301 cagacccggc ggtgcctcgc gcttccagcg ccgatcggtc aggaacatgt aggtcacgac
      361 ccgatgccgc ccgtcgtcca gcgcgacgta gcggtcgagc agtcgctgca gcccttgctg
      421 ttgcaggtcc tcgattcgca cgggtcgctc ggggcggagg aacgtctcca accggtcggt
      481 gaactcggag aacgcgtcgg gacgaaagcg ctgctgcgcc agccccgacg cgagcgcggc
      541 gcggatccgc tcgacgtcga atgcgcccgt ctcgtcttcg cgtagcgccg cgagcacctg
      601 gagctgctcg tcgagcggcg gaaggtagct cagcacggaa tcggacgagg ccaccgttcc
      661 gtcctcgatg aacggctgca gccgctcgtc gatccggctg gtcagcgcga tcgcctcgtc
      721 gagcgtctcg ccctcggcga tcgccatcat gtaggacaac gaggcgccga acatcttgcg
      781 cacgtcgtct tggaccttga gcgcttccga tcgctcgctg cgcagcaccg tgatcgtgtc
      841 gtcgaagccc aaccgggtgg cgaggtagcc gctcaccgcc gtgatcagcg ccaggctgac
      901 gagcgtcagt cggcggtagc gcaccgacag ggtgatcagc cgctccagac cgaacgattg
      961 caggtgcagc ttccgcaacg agtcggtccg acgcgggcgc acgccctcgt tccacttgat
     1021 catcgccggc agcatgaaga cgatcgcgac ggcgcacagc agaatgccgc cgccgatcag
     1081 gaagccgaga tcccacagcc cctgaaactc gctggtgcac atcgcgtaga acgtgccggc
     1141 cgacgtgatc gcgccggtga acacgcccag cccggtctcg ccgatcatca actcggtcgc
     1201 ctcggccagc gtgcggccgc gccggcgctc ttcgacgtag cgcgcataca tcacgatgac
     1261 gaagtcggtt cccagaccca tcagtaacgc ggtaaaggcg gaggacgacg cgttcagttg
     1321 gcgcagcgcg aagaacgcga cggcgaaggt cagcgcctgg ccgaccaaca gcggaagact
     1381 ggagtagagc agcgcggcga agcgccggta gcacagccag tagagcgccg aaaccgcgat
     1441 cagcgagacg atcaggttgc gcgatacgtc ctgccggatc agcgcctcct cgtccaacgc
     1501 gatcgcgtag ttgccgccgt agcgcacgcc gacgcgatgc ggaccgccct cccccgccga
     1561 cgcttccagc gcgagctcgc ggcgtacctc gtcctcgagc gcccgcaccg cgcccatcag
     1621 cctgcggtcg aactcgaggt tctgcgacgg cccgaccggc ttgaccagca tgatcagcgt
     1681 gcgcgcgtcc tgtgacaggt agtagccgtc ggacagatcg accttgagca ccccgcggtt
     1741 gccctcgagc cgattgagaa acagcggcat cagccgcagc gggtcgttga cgacgaggtc
     1801 ctgggtcaag gacgccatcg gcgagctcag ctcgagcttc aagcgcgaca gccgttcggc
     1861 gatcgcctcg tcggtcagct tggccgccag ctcgcccagc cgatccggtg gcaggaacag
     1921 cacggcgttg cggtagaaca gctcgaggaa atcgtcatcg agctcgaatc ggtactcgac
     1981 gtactcgatc agctcgtcga gaccgaccag tcggtcggcg aaacgatcgg cgaagtcttc
     2041 cagctcgtcc ggcccctcgc cctcgccggc ctcgagcagc acgagcaggt agtcgatcga
     2101 gccgaaatcg gccaacgcct tgcgcagcgt atcgacctgg cggttgccct cggggatcag
     2161 cgcgaggatg tccgactcga gcttcagccg cgagccgagc cagatcccgc cgaccagcgc
     2221 caacaacgcg agcgagaaga cgacgccgta gtgctggcgg ctgaaacgct cgattcgcag
     2281 cagaatccgg tgcttgtgcc cgagcttggg cgggccggcc gacggcgtcg tcatcctcca
     2341 cgggttcctg tcggcacgtt catccgcgcg attattgaat tacgctccgc gtgcgtcaag
     2401 cgcaggcgcg cggcgcacgg tccgccgcgc ggcgggggtc gcgcgtgcgt ggtattctgc
     2461 tgaacttccg cccccaggtc ggggagccgc ctaccgaggt gtgacgatgg agacgacgcg
     2521 agccaagacc gagcagttca tccgtcaaga ggacaagtac ggcgcccaca actaccatcc
     2581 gttggacgtg gtgatccacc gcgccgaggg cgtgtgggtc tgggacgtcg agggcaatcg
     2641 ttatctcgat ttcctcgccg cctactcggc ggtcaaccaa ggtcacgccc atccacgcat
     2701 ccgccaggcg atgatcgagc agagcggtct cgtcacgctg acctcgcgcg ccttccgcaa
     2761 cgaccggctc ggcccgctgt acgagcaggt ctcgcggatg accggctacc accgcatgtt
     2821 gccgatgaat accggcgcgg aggcggtcga gacggcgatc aaggcggccc gcaagtgggg
     2881 ctacaaggtg aagggcgtcg ccgccggcaa ggccgagatc ctcgttttca ccggcaactt
     2941 ccacggtcgc accacgacga tcgtcggctt ctcggacgac gcgcagtatc gcgaaggctt
     3001 cgggccgttc acgcccggct tcaccttgct gccgtacggc gacctcgccg ccgcgcgcca
     3061 ggcgatgaac ccgtcggtgg tcggcgtgct ggtcgagccg atccagggcg agagcggcat
     3121 cgtcattcct cccgccggtt acctcgaggg gctcgagcag gcctgccgcg agcacaacgc
     3181 gctgttgatc tgcgacgaga tccagtccgg gctcggccgg accggcaagc tgttcgcctt
     3241 cgaacacgag ggcgtgcggc ccgacatcgt catcatcggc aaggcgctgg ccggcggctt
     3301 ctacccggtc tcggcgatcc tggccgacga cgaggtgatg ggcgtgttcc accccggcga
     3361 ccacggctcg accttcggcg gcaacccgct cggttgcgcg attgccggcg cggcgctcga
     3421 cgtgctcgtc gaggagaaga tgatcgagaa ctcggcgaag ctgggtgctt actttctcga
     3481 caagctgcgt tcgatcgaga gccgcaacat cgaggagatc cgcggtcgcg ggctgtggat
     3541 cgggctgcag ctgaccaagc aggccggcgg ggcgcggcgt tactgcgagg cgctcgaacg
     3601 cgagggcctg ctgtgcaagg aaacccacgt cgatacgatt cgcttcgcgc ccccgttgtc
     3661 gatcacgcgc gacgagatcg actgggcgtt ggagcggatc cgcaaggtct tcggcgaact
     3721 cggctgatgg ccggtcaggc ttcgaccgtc gacatcgagg gggtcgcgct tcacttggcg
     3781 cacccggacg agatcgcgat cgagtgggtc ggccagcacg agacgatgag acaactgctg
     3841 gccgcctgga gcgtgatcga ccagcgtgac gtgccgatga gcccgcggct gctcggcaaa
     3901 ccgggtgtcg gcaagacgac gttggcctat gccgccgcca agcggctggg gcgcgatgcc
     3961 tacatcttcc aggcgacgat ggacacgcga cccgaggatc tgctgatcac ccccgtcgtc
     4021 gacaagcagg gctcgatccg ctacgtcgca tcgtcgctcg tgaccgcgat gatccgcggc
     4081 gacgttgcga tcctcgacga ggggaaccgg atgtcggaga aatcgtgggc cagtttggca
     4141 ccgctgctcg acaaccggcg ctacgtcgag tcgatcgtcg ccggggtcaa ggtcgcggcg
     4201 catgcggagt tccgcatcgt ggcgacgatg aacgacgacg cctcgaccta cgagcttccc
     4261 gagtacatcc actcgcgact gcagccgcag atccacctcg attttcccga cggcgacgag
     4321 gaaaagcgga tcctgcaggc caacctaccg ttctcggacg accgcttgct ggactacgtc
     4381 acgcggttcc tgcaagcggc acacgcggcg gacgagacct acaccgtccg cgacggtgtc
     4441 aacgtcgcgc gctacgcgct gaagctgatc gacgggcgag ccgcgagcga cgcggcctgc
     4501 cgatcggcgc tggccgaggc gatccggcga atcctcgacg acgacgcgct gcgccacttc
     4561 gaccggtagc gtgtcggcca tcgccgcggt cggtccggcg gcatcctcca tcagggttgc
     4621 ggataacccg ggcgctgtcc cagcggcgtg ctgagcaata ccgtgatctg atcgagccgg
     4681 accgccagtt ccgcccgtgc ctgctcgatc cgcgcctcga gcgccgcttg actcgcgagg
     4741 atctcttggc gattccggtc cagcttgtcc gccagctcgg tcgccgtttg atcgagcttg
     4801 ccgttgacga cctgcagact cgcgatcgtc tcctgcagac aggcgaaggt gcgatcctga
     4861 acgagggacg tgcgggtctc gacctcggcc tggaccgctt tgtgcgcgat gtcggcgagg
     4921 ccctccgccc cggcgaggat cgcgctcagc gccgagccgt tcccgccgcc gccgccgccg
     4981 aagccggccg caacggcgac gacgacgacg gtctggttgt tgagcgcggc tccgatgtcg
     5041 gcgaccatcg ccagccccgc gtgtgcccca cgcaggaagt acatcgcctc gaccagtccg
     5101 tccgggagcg cgtagatgtc atcgcacgat tcggccgacc ggtcgaccgc cgacgcgtct
     5161 tcgacgagca gcgcgttgac gaatgccaac cggtcgttca cttcgaggac gctcttctcg
     5221 atttcctcgt acgtacgttc gaggtcctga gcgacgtgct cggcctcctg ctcgtcgctg
     5281 acgcgcagcg cccacgtggc gcagttctcc gcgcagatgt accgctcgtt gttcgggttg
     5341 tccttcttcg cccgcggatc gcagctcttt tcggggtagt tcagcgcctg gcattgctcg
     5401 tccggccagt cgcagatccc gttgtcgttg tcgtcgacca acggaaccca ctcgcagcct
     5461 tcggccttcc ggtacttggc gacacccttg gcggcggtct cgaagtcgtc cacgcgcagc
     5521 ttgccgtgga gcatgcgtgc ttcctgtttg atccgcgcgg cgaggtcgcg gcgttgggcg
     5581 gtcatcccgg gcgacgaggc cagcggggag cccgtcaagt tgacgtcgac caggtgttcg
     5641 agctgggcca ccacggcgtc ctgcctgcac agcgtgcggg agtagacgtc ggggtccgcc
     5701 gcggagtctt cgggcttcgc accgcaatcc ttgaccgggg gagacgaagg ggtgtcggcc
     5761 gcgaccgacc ccgtcatcgc gatcatcggg gccgcggcga acaagatcag catcagcgcg
     5821 tgaaccaggg atcgctctct tcgcgacatg ctcatgacgt gtgactcctt tcgtgcgtct
     5881 cgtcgcttcg ctgtcggacc ccgcaggagt cggtcgtcga taccacttcg tcggaggcgc
     5941 ggacccgttg gcaccagtgg atccctccgg gcccgcgctg tcaacgtcgt ttcaccggca
     6001 attcggtccg ggtcggtcca caatccgacg gcggcttggc cctcgtcgaa cccgagccct
     6061 gccccgtcgt acactcgacc gggaggacaa ggacgatgac ccgacaggta ctgctcacga
     6121 ccgtgcgcgg cgcgctcgcg gcgatctcgc tgacgatggc ctgcgctccc gccgatgacc
     6181 cgccggtggc cgagacgatg ccgcctgcgg acatcgtggc cgacggaccg ctcgcggcaa
     6241 ggatcgacgc ctacgccgct cccctcgccg cgcgcgggga gttcagcggc gtcgtgctcg
     6301 tcggctcacg cgacgcgatc ctgttcgcca gagcctacgg catgtcggac tacacgagcg
     6361 acgcacccaa ccggctggag accggtttcc gcgtcgcctc gatcaccaag acgttcacct
     6421 cggcggccgt tcttcgacta cgcgacgagg gcaagctcga gctggacgat cggctgagcc
     6481 gtttcgcgcc cgacttcccg cgcggggacg agatcacgat ccggcatctg ctgctccatc
     6541 gctcgggctt gcggaacccg gactacctgg aagccttcga gcggagcatc ggtctcgacg
     6601 agctggtcga tcgcattgcc gcgcgggggc tggctttcga ccccggtacc gacggcgcct
     6661 actcgaacgc cggctacaac gtgctggccc acgtggtgga gcgagcgtcc ggaacgacct
     6721 acgaagacta tcttcgccag acgattctcg ctccgctggg gatggagaca accgaccacg
     6781 acggctcccc cgcccgcggc tacctgccgg ccccgccgcc ggtgattcgg gtcgtcccgc
     6841 cggaggcgga cccggtcggg ttcatgatcg gcagcggctc actcgtgtcg acggcgcccg
     6901 acctgtggcg atgggcgcgc gcggtcgccg acgaacgtat cgtcggctgg aaggacctcg
     6961 agtggcccta cggctggggc agggtcgaga tcggcgagca cgaggggatc gagcagaccg
     7021 gggccaccac cggcttcatg tcgagcctgc tcgtcttccc cgacggcgat cgattcgtcg
     7081 tcgtgttgca caacgtcgag tacggaccgt ggacctcgtt cggcaaggac atcgccgcca
     7141 tcgccttcgg cgaggagcgc gcgccgccgc cggcccgcga gccgtacgcg ctcgaccccg
     7201 gctcgctcga gcaatacgcc ggacggtatt cggaggcgga cacgacgctg acggttcgcg
     7261 ccgaggaagg acagctgcgg atgtactaca acgactggcc gatcggcaag tatctcgtcc
     7321 cgacctccgc gtcacgcttc gcgccgttgg gcgacggggg cgagatcgtc ttcgccggcg
     7381 agcgcgacgg cgtcttctcc gagctgtcgt ggcgcttcgg cgactcgggc acgacctacc
     7441 gccgcggcga ccggtgatcc ggcgcgcggt gcgtcagtcg acgcggtccg tctcccgcac
     7501 gaagcgtacc aggtcctcgc ggtactgggc catcgacggc ggatgcaggt acatcatgtg
     7561 gccggcttcg tagtacgcca ggcggacgcg ttgcctcgcc tcgtcgggga tgtccaggtg
     7621 ccgcacgtag tagtcggcga tgaagtaggg cgtggccagg tcgtagtaac cctgctggac
     7681 cagcacgccg agtccggggt tcatcttcat cgcatgcgcg agatccggca ggacgttggg
     7741 gaacggcggt gcgaagacgc cgtcggaccc gggctgtgcg tgcgcccagt cccattcccg
     7801 ccaggcccgg gtcatgacga cgtactcgtc ctccaccgcg aactcgagct cgcgccggta
     7861 gtagtccttg aaccccgcgg tgaacgccgg cccgatcgcg gggaagaacg ggtcgtagtc
     7921 ctggctctcg gccagcgggt tgagcgccgg tccggtgaag cgggcgtcga tccgaccggt
     7981 ggtcgtgccc cggtcgcggc cgagctcttg caggaactgt ccgtgcgaaa cacggaggtc
     8041 ggccttgagc cagtagtcgg tgctcgtacc ggtgaacgcg gcggcccggg cggcgatctc
     8101 ccgcttctcc tcgagtggca gcgtgtagcc gcgcaacagc gcaggcagat actcgtcgat
     8161 cgcgaagcgc tccacctcgt cgagaaactc gcgtagtacc ggcgcagcgt cgtcgaggcg
     8221 ctcgtggtac caggcggccg cggccagccc gggcagatag agcgcgtgcg gcaggtcgag
     8281 accgatgccg tcggcgccga agccgaagtg catgaacggc gaaaccagaa ccacgccgtt
     8341 gatgctcatg cgatgggtgt cctgcaggtg gtaggcgagc ccggcggcac ggattccgcc
     8401 gtagctttcg cccaggatgt acttcggcga gcgccaccgc gcgttctgct cgacccaccg
     8461 gacgatgaac cgcgacaccg agcggacgtc ctgatcgacg ccccagtgat cctcgatctt
     8521 gccttggccg aggaccttgg agtatccggt tccgaccgga tcgatcatga cgatgtccgt
     8581 cacgtcgagc acggtatggg cgttgtcgac gatcccgacg ggcggcggcg gcgtgaaccc
     8641 ggggtcggcg accaccacgc ggcgcggtcc gaggatcccc atgtgcagcc agatcgagga
     8701 cgagccggga ccgccgttga aggcgaacag gaccggccgc agcgacggtt cggagacgtc
     8761 tttgacggca taggcggtgt agccgaagcg ggccgtcggc tgaccggaat cgtcgcgcag
     8821 gatgagccag ccggcggtgg cggtgtaggg gatcgacttg ccgtcgatcc gcagctggtg
     8881 tgtggtgacg acggcgacgg gctcggccgg cggcgacttg tcgtcgggcg gcagcgcgcc
     8941 gggaagcaac aacgtcaggg tgatcgcgac ggaacggacc gaactgcgca tcgacgtacc
     9001 tctccgtccg gggcgacggg atcgagatcg aatggtaaca gacgtcggcg gcatggagtt
     9061 cgcaagaacg atcaagctcg cgccgcggac tcctgctagg ctcggaggcg tgatgcgggc
     9121 cgagacggag atcgactatg ccgagcggat cacgcgagtg ctgctccaca tccaagggaa
     9181 cctcgatgcg ccgctcgacc tggagcggct cgccgcgatc gcccatttct cgcccttcca
     9241 cttccatcgc gtcttcagcg ggatggtggg agagagcgtg aagcgtcacg tgagacggct
     9301 gcggctcgaa cgcgccgcga tgcgcctcaa gcacgcgcgc cagccggtga cgcgaatcgc
     9361 gctcgaggcg ggctatcagt cccacgaggc gttcacgcgg gcgtttcgct cgttgttcgg
     9421 gttgtcgccg acccagttcc gcgccgcgac cgatccggtc gcgcgggccg gcgcacaggc
     9481 tcgcgtgcgg tacgcgcccg acggaagcgt ggccggcttc gcctcgatcg gcccgaacga
     9541 cgtgccggtc gacgtcgaga tccgcgagtt ggcgccgatg cgggccgcgt tcattcgcca
     9601 tgtcggcccg tatgacgagg tcggttcgac gtgggagcag ctgatcgact gggtcggccg
     9661 tgaatgctcg ttcgggccgc ggctgcgatt cttcggcgcg tgctgggacg atccggaggt
     9721 gacgccagcg cggagcctgc gctacgacgc ctgtgtcacg gtcgacgacg acgtgaccgc
     9781 ggcgggagcg gtcggcgttc agcgggtcgc cggcggtcgc ttcgccgtcg cgctgcacga
     9841 gggcccgtac cgcgggttgg ccgccagcta cgcgaagctg tgcggattgt ggttccctgc
     9901 gagtggcgag cgactgggcg acccgccttc gctcgagttc tacctcgacg atcccgagtc
     9961 gactccgccc gaggagctgc tgaccgaagt gtggatgcgg atcgccgatt gaacggggag
    10021 gatgacgatg gccagcgaaa cacccgagaa gatcgacctg ttcaagctgc acaaggacga
    10081 ctaccgggcg tcgggcacgc cggagctggt tttcgtcggc acggcgaact acctagtcgt
    10141 ccgcggtcac ggcgagcccg gcggcgagtg cttcgagtcg cgggtccagg cgctgtacgg
    10201 catggcctac acgctgaagt tcacgggcaa ggccgcgggt cacgacttcg tcgtgggcaa
    10261 gctcgagggt ctgtacggcg tcgacgggca gtcgatcgac gatctcgcgc ggctggcacc
    10321 gcagcggtgg aactggcgtc tgatgatccg cgtgccggac cggatccgcc cgcagcacgt
    10381 gaccgaggcg aggcggacgc tcgacgagaa gggcaaggcg ggtgacttcg acgcggtgac
    10441 gctggaatcg atcgacgagg gccgctgcgt ccagatgctg cacgtcggac cgtacgagca
    10501 agaatcgcga accgtcgcgg cgatgcggga gctggcccgg cgaaacgggc tcgcggcgca
    10561 cacgtggcac cacgagatct acctcagcga tccgcgtcgg gttccggcgg ccaagctacg
    10621 caccatcctg cgcgagcccg tgcggtaagc gaccgacgag cctccgccgc gtacgacggt
    10681 cggactacgg gccgtcgcag gcgtcgcccc ggccgtcgcc gtcgctgtcc gcctggtcgg
    10741 ggttgggaac gtcggtgcag ttgtcacaac gattgccgac cccgtcgccg tccccgtcgg
    10801 cctggttctg tgtcggcagg tcgggacagt tgtcgcacgc gtcgccgaag ccgtcgccgt
    10861 cccggtcgag ctgaccgatg ttggccacgg tcggacagtt gtcgacttcg tcgaagtgcc
    10921 cgtcaccgtc gccgtcaccc aacagctggc agtcgtcgcc gactccgttc tggtcggagt
    10981 ccgtttgata cggattgaac tgccccgggc aattgtcggc gcagtccagc aggccgtctt
    11041 cgtcgacgtc ggtgtccggg acgaagcagc cgcagacgcc cgcggcgaac ttggccggat
    11101 cgctcggaca ctcgtcgcag acgttgccgg caaagtcgag gtccccgtcg gcctgggtca
    11161 cgtccggcgt cacgctgcag ttgtcggcct tgtcctcgac gccatcggcg tcgaagtccg
    11221 cgaagccggc ggggcactcg accgtcaccg acagcggaat cgtctcgctc ccgtccccca
    11281 tcgggccgtc gccgcacagg ttgcggccgg cgacgaggta gtagatcacc tcgccgttcg
    11341 ccggcgggat cagatcgtcc gtcgcggcga gacccggcac ctcggcggcg aggcagctcg
    11401 gcgagcccgg ttggcggtcg agaccgatca agccgcgata gacgttcgag gtgtgtccgg
    11461 ccgccccgcg gccccacgac agaacgaggc cgttcccctt gcccagccgc aggctggagc
    11521 cgaccccccc cggtgcggcg gcgacgccga cggtcgccgg cgcacagtcg tagacgtccg
    11581 gcacgccgtc gttgtcgccg tccggcaacg gatcacacgc gtctcccgga ccgtcgccgt
    11641 cgccgtccgg ctggcccggg ttgaaggtgc tcgggcagtt gtcgcacgcg tcaccgacgc
    11701 cgtcgagatc gaagtcggct tgatcggcgt tcgccgccgc gggacagttg tcgcacggat
    11761 cgccgtcgcc gtcgccatcg aggtcggact ggctcgtgtt cgcgatcgcg acacagttgt
    11821 cgagctgatt gatcacgccg tcgccgtccc gatcgccgtc gggatcacac gcgtcgccgg
    11881 cgccgtcccc gtcggcgtcg gtctgggcgg ggttgggcac gctgccacaa ttgtcgcact
    11941 tgtcgcccac gccgtcggca tcgccgtcgg cctggttgac gttcttgtcg agcggacagt
    12001 tgtcgcaggc gtctcccgcg ccgtcggcgt cgccgtcgcc ctgcgccggg ttgggcgaca
    12061 tcgggcagtt gtcgtacgcg tcgcccacgc cgtcgccgtc gtcgtctccc gcgacctcgc
    12121 aggcgttgcc gatgccgtcc tggtcggcgt cgagctgcgc cggattcgcc accgacgggc
    12181 agttgtccac atcgccgcac agcaggtcgt ggtcggcgtc atcgtcgggg tcgttcgggc
    12241 actcgtcgca cgcatcgccc ggcccgtcct ggtcgctgtc gagctgcgcc gggttgtcga
    12301 ccgacggaca gttgtcgacc tcgccgcaaa caccgtccgc gtcgaggtcg ttttgcggat
    12361 cggcgggaca gctgtcgcga aaatcgcagc ggccgtcccc gtcggtgtcg gcgtcgggac
    12421 actgcttgag gatggtgaat cgatcgcgga cggtcgcggt cgaatcgatc aagctcgcat
    12481 cgagccgatt gccgtcgacc tccagcacga ccgagccgag gatgttcagc gagacgatca
    12541 tcgccgggtg atcgagcgcg ccgccggagt acatccccga gctgccggcg acggtgtaga
    12601 ccgacccgtc tctcggtgtc gatccgtagg gcgttccgct gcgcggagcc ttggtgtacg
    12661 cgccgtcccc ggccgggtcg ccgtcgccgc tgtcgatctt catcccttcg acgaaagtgg
    12721 tcgaatcccc atagtgcccg tcgatcaggt aggaacgctc gtaggagtgg ctgtgtcccg
    12781 cgagcaccag gtcgacgccg tagtcctcga gaaccggaac ggcgttctct cgcatttcga
    12841 ccagctcggt atcgaagccc gacggattgt cggagtcgtg agagcccttg ctgtagggcg
    12901 ggtggtgcca gaaggcgatg atccagtcct gtgtcgtcgc cgccagatcg acctcgagcc
    12961 atgtcagcat cgcgcttccg agcagacggt tcgagaccat cgagtcgagc acgacgaagt
    13021 ggatgttcgc ccagtcgaac gagtagtact gctccgtgcc cgaggacacc ccgccggctt
    13081 cgccggccgt tggcagcgtg aagatgtcgg cgtagggcca ggtgccgccg tccggcaggt
    13141 cgtggttgcc gatcgtcggc cagagcaccg aggtgcgcaa cctttccggg tagatctcga
    13201 acatcgccgc ctggtagtcc gcgtccgtgc cgtcggggta ggcattgtcg ccgagcatca
    13261 accaggtgtc ggtgtgcgcg gttccgctga cgaactcgta ggcgtcccgt acgtcgatcg
    13321 cgccggccga ggcggtgccc gagtctccca gcacccagat ccgtgtcggc gccggcgtgc
    13381 cggcgagcgg cggcgtgacg aagtagtggc tgccgtcgtc cggcgtcaac acctcggaca
    13441 gcgtcccgac gccatagcag tagtgggtgc cgggggtcag gccggtcagg cgcacctcgt
    13501 gctcggtgtc gaggatcaga tccgagacga tcgagttgaa acagctcggc ggccggccgt
    13561 agtagacgac gctttccgcg gggacgtccg tccgccaacg cacgacgacg ctcgtcggcg
    13621 ttcccgactg gaggtagggc ccgcgcacga tcccgatctc gctcggggtg ccggcgcgag
    13681 ccggctggac aagcggcaac agcgcggcga gcacgacgag cgcgggcacg gtccgccacg
    13741 acgcctttgg gccgtcaacc ttccgcaccg atcggttccc ccggagtctc tgcggaggat
    13801 tacttaccac caagaggtgg aaacggccac cgcgaccgcg ggggtccggg ctagcggcgg
    13861 acgtcgatca ggttgaggat cgcccggaac cggggatggt cgcgcacgga gtcgaagtcg
    13921 ctgtcgtttt ccagccaagc cttgtggttg tagcccgccg cgacggcgcg ctccaggtgg
    13981 tcgagcgcct ccttcgtgcg ttcgatcgtg gcatagatgc aggcgacgtt gtagagcagc
    14041 ccggtttcgt tgggggcgat cgtcaccgcg cggtcgacca gctccagccc ggcgtccacc
    14101 tctccgacct cgaccagcgc cccgccgcag aggtagagcg cccgcacgtc gtccgggttc
    14161 agctcgaggt gccggcgggc cgcgccgatc ccggccagct gggcctggtt ggcgtccgcc
    14221 cggcgctcga ggcggatgta gacctggggc agcagcagac gagcctggta gtcgtcgggg
    14281 cggacgtcga tcgccttctc gtacagctcc gcggccttgg cgaagttgcc ctgcccgacg
    14341 ctggcacgag cgtagaagta gtaggcgtcg tacagcttgg cgttgagctg gatcgcccgc
    14401 tcgaagtggc ggccggcctc gtcgtgctta tcggcgagcg acagcaccat cccggccgac
    14461 gcatgcgcct cggcgagatc cggatcgagc tcgagcgcct tggcgctggc ctgctcggcg
    14521 aactgtcgct cgcgctcgct gctgccggaa tacatgaaca ggaacgacca gcagtcggcg
    14581 atgccggcgt aggccagcgc atagctcggg tcgagctcga tcgcgcgctc gaacatctgg
    14641 cgggccagct cccagttgcg acggtcgaac tcgtagaagt acttgcgccc gcgcaggtag
    14701 aagtcgtagg cccggacgtc gcgggtcgcg acgttctgga tcgcgcgccg ctccttcgga
    14761 ctcagcgtga cctccagcgc ccgcacgata ttctcggcga tctcgtcctg gatggcgaag
    14821 atgtcttcca acttgcgatc gtaacgctcg gaccacagac ggtagccgtc gctgacgttg
    14881 atcagctggg cgttgatccg cacccgatcg cccgccttgc gcacgctgcc ctcgacgacc
    14941 ttaccgacgt tcagccggtc gccgatctcg cggatgtcgt gcttggatcc cttgaactgg
    15001 aaggccgagg tgcgcgaggc cacgcgcagg ttcttgatcc gccccaaacc gttgatcaac
    15061 tcttccgcga tcccttcgca gaagtactcc tgcgcgggat cggggctcat gttggcgaac
    15121 ggcagaacgg cgatcgagtg gacgtcggga tcgacggaga cgcgctcgtc cctcagctcc
    15181 ttcaactcgt tcaacagatc gcgtgcggtc tgatagcggt cctgcgggtc tttgttgaga
    15241 catcgtcgga tgatccgacc caggtggttc ggcaggccga cacggacgtc cgagaccggc
    15301 tccggcgtgt cgcgcaggat cgaggagatc aggtcggccg agctcgcgcc gcggaacggg
    15361 cgctttccgg tgatcaactc aaagaagatg acaccgagcg agaagatgtc cgagcggtgg
    15421 tcggccgcgc gcccctgtac ctgttcgggc gacatgtacg gaaccgtccc gacgatcatc
    15481 ccgtcctggg tcagcccttg ggtctggatc ccggtgatgt cgtccgcgcg cgggccgtcg
    15541 tggagcttgg ccagaccgaa gtcgagcacc ttgacccgac cgtcgtcgcc gatcatgacg
    15601 ttggccggct tgaggtcgcg gtgcgtgatt cccttttcgt gcgcggcgca gaccgcgtcg
    15661 accagcggcc gggcgatccg caggaaatcc ggcaacgcca tcccgcccgg gtcgacgcgc
    15721 tgctcgagac tgcgaccctc gaccagctcc atcgtgagga agtgcaggcc ctcgctctcc
    15781 tcgaccgagt ggatcgtcac gatgttcggg tggttgagcg ccgcgatcgt cttggcctcg
    15841 cgctcgaagc ggcggagtcc gttggggtcg ccggcgagcg ccgccggcag caccttcagc
    15901 gcgacccggc ggtcgagcgt cgcgtcttcg gcgagataga cctcgcccat tccaccggaa
    15961 ccgagtcggt cgaggatctt gaagcgcgcg agcgtcttgc cgatcaggtc gggttgcgcg
    16021 ttcacttgga cgctccgtcg caccgtcgaa agaggtgcga ctaagaatac ggggggaccc
    16081 ggggacggtc aagaattgcg gagcgtccga tcgtcgcacc gtcgccggcg ccggacgacc
    16141 gagcgctcac ctcccttgag cgggtcgcac ggctgagtta cgctcgcgcg accgtgggcc
    16201 gaggtgatgc gtggcgaacg tcctcgtgat tcaaccgggc ttcccgcttg agatcccgta
    16261 ctacgtgcgc gggctggccc gcgccggggc ccgcgtgctc ggcgtcggcg accagcccgc
    16321 cgagagcctg cccgcgccgg cacgggaatc gctcgcggcc tacctgcagg tcggcaacct
    16381 gtgggacgcc gcgggcctga tcgatgcgct gcgccgctgg gacgtgcctg tcgggttcga
    16441 ccgggtcgag gccctgtggg agccggcgat ggagatcgcc gcggagatcc gccgcgcgtt
    16501 cgggctcccg gggctggacg ccgagcacac gacctggttt cgcgacaagc accggatgcg
    16561 acagcgcctc gagcaggctg gagtgcgcaa catccgcttc gcccgcgtgc gaggcgccga
    16621 cgaggtgccg gaggctgccg aacgggtcgg cttcccgttg atcgtcaagc ccgtggccgg
    16681 tgccggcgcg agccacacct accgcgtcga cgggcgggac gagctgatcc gcctgctgcc
    16741 cgcgttgggc tccgtcggcg agctggccgt cgaggagttc gtcgacggca ccgagttcac
    16801 cttcgacacg ctgtgcgccg ggggccgcat cctgttccac aacatcctgc gttacacgcc
    16861 gaccatgctc gagtcgcggt cgaacgagtg gatcagcccg cagaacatgg tcctccgtga
    16921 tctcgatcgc cccgagttcc ggcgggcctt tcgtctcggc gagcaggtgc tgcgcgcgct
    16981 cgagttccgc agcgggatca cccacatgga gtggttcctc aggccggacg gcgaggcggt
    17041 cttctgcgag atcgccgccc gccctcccgg cggcatgacc ggcgagctga tgaactactg
    17101 ctgcgacttc gacgtctacc gcgcgttggg ctcgacgatc ctccacggaa cgatcgacca
    17161 gccgatcgag cggaagtaca acgtggcgat ggtcttcaag cgcgcgatcg gacagggccg
    17221 catccggaag atcgaagggc tggcggagct ctaccggcgg ttcggcccgc acgtcgtgcg
    17281 gcacgagctg ctgccgctgg gcgcgcggcg acgcgactgg aagcagacgc tgatctcgga
    17341 cggcttcgtc atcctgcgcc accccgacct ggcgcaagcg gaacggatgt cccgcttcgt
    17401 cgcggaaaac gtgcggctct acgcgggcta gccgtcaaag gtcgagcggg aagaaggcca
    17461 gcgtgagccg gaagcgccgt ggtttgccgg gcccgcccga ctcctcgtcg cagcccgtct
    17521 cgccgacatc gtgcagcacc gccatcagct tctcgcggag tcgccggatc tgttcctcgc
    17581 tcgcgtgcac ctcggtgtag ctcagcaacg cctcgtcgga gccggcgacg tcgtggccgg
    17641 cgtcgatcag gcgccgcaga tcctcggcgg tgttgagcat catcgtctgg accactccga
    17701 ccacctgctt cgccgcctcc cccgcgtcgt ccgtgcggaa cagactcgag tcggcgcgaa
    17761 agaccttggc gacggcgcgg tagtacttct cgacggtgcc ccgcacctga cgggtctctg
    17821 ccaagcggat caagcccacc cgctcgagcg cgtcgacgtg gtggtacagg cgtgtcggct
    17881 tctcgcccaa ccgcttcgcg acctgcttcg tcgtccgcgg ctcgtcgtcg cacaacgcct
    17941 cgaggatccg cacgcgcaac tcgtcggcga gcaccttgac ctgctcgagg tcgcggagcg
    18001 tgtagacctc tgcccgatcg ctccgatcgt ccgctttcgg cttgatttcg ctcgtttgtg
    18061 gtccctccga cggagatttt accagatcct ggattgaatt ctattattca gttagatatg
    18121 aataatacgt agttatcttt taatcaaatt atccagaacg gggggtcccc atgaaggtcg
    18181 attgctcgga acgccgcttg gcgccgttct tcgtcgtatg gagcggccag gccgtctcgt
    18241 tgatcggtac gcaggccgtg cagttcgccc tcgtctggtg gctgaccgtc cggaccggat
    18301 cggccaccgt gctcgcgacg gcgaccttcg tcgcgctcgc gccgcagatc gttctcggac
    18361 cgttcgtcgg cgcgctggtc gaccgctgga accgcaagtg gatcatgctc gcgtccgatg
    18421 gcgcgatggc cgctgcctcg gcgctgctcg ccctgtcgtt tctcgacggc agcgcgacca
    18481 gcgggcaggt gttcgccttg ctcttcgtgc gtgcgttggg tgcggtgttc catggcacgg
    18541 cgatgaccgc ggcgacgtcg atgatggtgc cggtcaccca gctgacgcgg atccaaggtc
    18601 tcaatcagat gctgcag
//
LOCUS       DASPGN010000015         4279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_237569, whole genome shotgun sequence.
ACCESSION   DASPGN010000015 DASPGN010000000
VERSION     DASPGN010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237569"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VD788_00500"
     CDS             complement(<1..722)
                     /locus_tag="VD788_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74768.1"
                     /translation="MDDSNEPPPPAHVPPPAAAYRWLGVFESLAVLLACVAVILALSV
                     AELWLLAPRLYTAEFELVEQRHVGDGRASTTEELLEQLSRLGLDADADVRERRGRTLL
                     SISGLRSADTIDGTVNGLLVESGFDAAEGRIRTGLDWQGMITRHPVLILGAQAIVLIA
                     FGLAFSRLRVGPARERPRARASVAVAFGVAAGLAGFACALILGLLQELLGLSIEEQAW
                     LLELLRDRDSLFAVAPLVAVVAP"
     gene            complement(931..1602)
                     /locus_tag="VD788_00505"
     CDS             complement(931..1602)
                     /locus_tag="VD788_00505"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXV74769.1"
                     /translation="MLALLDHGNEARLKWTALGIAIAIHLVLMLINFPEIRQAVQPRP
                     QGPVLIVRKYIPPPPKLERPQRTVVRRELTRRIPIPDPTPDEPEPIREPEPDIVVPPL
                     PEDVEVLIGVPEPPGPGPGEGPRIAGTGGVTMPALIPESKIAPQYPELARVARIEGRV
                     ILQAVICPDGSVSDLVVVRCSHPRLGFEEAAVDAVRQWRYTPATLDGRPVSVYFTVLV
                     DFELL"
     gene            complement(1640..1951)
                     /locus_tag="VD788_00510"
     CDS             complement(1640..1951)
                     /locus_tag="VD788_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HXV74770.1"
                     /translation="MPRPRKRRTCRAHSGDRVFKPRSAPMGRLETVRLELSELEAMRL
                     CDLEGLNQTRSGERMGISRGTVQRLLKSGRLKVVKTLLDSHALVIEEGGDHEDLFSEE
                     T"
     gene            2070..>4279
                     /locus_tag="VD788_00515"
     CDS             2070..>4279
                     /locus_tag="VD788_00515"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HXV74771.1"
                     /translation="MATIDSTPEFVEDAYRRIEEKLEIVRRRLGRSLTFAEKVLLGHL
                     DDPEHADLRTGESYIQLRPDRVAMQDATAQMALLQFAQADIPHVAVPTTVHADHLIRA
                     YRGANQDMQVAIDENNEVYAFLQSASARYGIGFWQPGAGIIHQVVLENYAFPGGLMIG
                     TDSHTPNAGGLGMFASGVGGADAVDVMAGFPWEVRYPNLIGVRLTGKLDGWAAPKDVI
                     LKLAGILTVTGGTNAVVEYFGPGAASISCTGKATITNMGAEIGATTSIFPFDASMGRY
                     LRGTERAALAELAERHPHLLQADDDVARDPDKFFSRVIEIDLSSLEPHLVGPHTPDLA
                     RPVSEVARAVEAEGYPNDISVALIGSCTNSSYEDISRVADVVGQAQAHGMQRAAIPLM
                     VTPGSEQVRATIERDGQMARLESFGATVLANACGPCIGQWQRDEIKPGQRNTIVTSFN
                     RNFPRRNDGNAETMAFIASPEIVAAYALAGKLEFDPRRDEVRVPGGAAFRLSPPAPAP
                     DLPADGFVRDASGYVEPPRDGASVEVRIAAGSQRLQRLAPFADWDGRDFDRLPLLLKV
                     KGKCTTDHISPAGPWLRFRGHLDNISDNMFTGASNSFTEKPGTAINTLDEDTVQPVPK
                     VARAYKEAGIPWVVIGDDNYGEGSSREHAAMEVRFLGGRAVIARSFARIAETNLKKQG
                     VLALTFDNPADYDKFGPHDRVSVRGLASFAPEQPLHVEIEHPDGSRDTITVRHS"
BASE COUNT          715 a         1479 c         1428 g          657 t
ORIGIN      
        1 ggggcgacca ccgcgaccag cggcgcgacc gcgaacagac tgtcacggtc ccgcagtagt
       61 tcgagcaacc aggcttgctc ctcgatcgac aggcccaaca actcctgcaa cagtcccagg
      121 atcaacgcgc aagcgaaacc ggccagcccc gccgcgacgc cgaaggcgac cgcgaccgac
      181 gcacgcgccc gcgggcgttc gcgagccggc cccacccgca accggctgaa cgccagaccg
      241 aaggcgatca gcacgatcgc ctgggcgccg aggatcagaa ccgggtgtcg cgtgatcatg
      301 ccctgccaat cgagcccggt gcggatccgg ccctcggcgg cgtcgaagcc ggattcgacc
      361 agcagcccgt tgaccgtgcc gtcgatcgta tcggccgagc gcaggcccga gatcgacaac
      421 agggtgcggc cgcgacgctc gcgcacgtcg gcgtcggcgt cgagaccgag ccgcgaaagc
      481 tgttcgagca gttcctcggt ggtgctggct cgcccgtcac cgacgtgacg ctgctcgacc
      541 agctcgaact cggccgtgta gaggcggggc gccaacagcc acaactcggc gacgctgagg
      601 gcgaggatca ccgccacgca ggccagcagc accgcgagcg attcgaacac gccgagccag
      661 cggtaggccg ccgctggcgg gggtacgtgg gcgggcggtg gtggctcgtt cgaatcgtcc
      721 acggtggcgg ttttctcctg gaccgtaaga ctctacacgt gccggccgcc gctggcttgc
      781 tttttctccg tccggatcta gattggactt cgcggcggcc gacggccgtc gcaacggatc
      841 gagtgagcat acggcatcca tctcccccca atggtgtcgt agcatccggg cgttcgtcga
      901 gaaatcggcg aacgccctac tttttcgcgc ttacagcagc tcgaagtcga cgagcaccgt
      961 gaagtagacc gagaccggtc tgccatcgag cgtcgccggc gtatagcgcc attggcgcac
     1021 cgcgtcgacc gcggcctcct cgaagccgag cctggggtgg ctgcagcgca cgacgaccag
     1081 atccgacacg ctgccgtcgg gacaaatcac cgcctgcaag atgacccttc cttcgatccg
     1141 tgccactcgg gccaactccg ggtactgcgg ggcgatcttc gactccggga tcagcgcggg
     1201 catcgtgacg cccccggtac ccgcgattcg cggcccttcg cccgggcccg ggccgggagg
     1261 ctcgggaacg ccgatcagca cctcgacgtc ctcggggagc ggcgggacga cgatgtcggg
     1321 ctcgggctcg cgaatcggct ccggttcgtc gggggtcgga tccgggatcg ggatgcgacg
     1381 cgtgagttcc cggcgtacga cggtgcgctg cgggcgctcg agtttcggtg gtggcgggat
     1441 gtacttgcgg acgatcaaca ccggcccctg gggccgcggc tgcacggctt gccggatctc
     1501 ggggaagttg atcagcatca gcaccaggtg gatcgcgatc gcgatcccca gggcggtcca
     1561 tttcaagcgg gcctcgttgc cgtgatccaa gagcgccagc atgccggcga ccgaggaacg
     1621 atcgggcccc gcccggatgt caggtttcct ccgaaaaaag gtcttcatgg tcgccacctt
     1681 cctcgatgac cagcgcgtgg ctgtcgagca gggtcttgac gactttcaag cgcccgctct
     1741 tcagcagccg ctggacggtt ccccgagaga ttcccatccg ctcccccgag cgcgtctggt
     1801 tcaggccttc gagatcgcaa agccgcatcg cctcgagctc gctcagctcg agccggaccg
     1861 tttcgagccg gcccatcggt gcgcttcgcg gcttgaacac ccggtcgccc gagtgggccc
     1921 ggcaggtgcg tcgtttcctc ggtctcggca tcggacccga cctccattcc gtgcatatgc
     1981 acaataccac attgccgtcg tgcactccag ggtgcggccg tttcccgctt tgtatagagt
     2041 gggcgcagtc acgcaaaggg gctcgaccga tggccacgat cgacagcact cccgagttcg
     2101 tcgaagacgc ctaccgccgg atcgaagaga agctcgagat cgtccgccgc cggctcggtc
     2161 gatcgctgac cttcgcggag aaggtgttgc tcgggcatct ggacgatccc gagcacgccg
     2221 acctgcgaac cggcgaaagc tacatccagc tgcgaccgga tcgcgtcgcc atgcaggatg
     2281 cgaccgcgca gatggccctg ctccagttcg cccaggcgga cattccccac gtggccgtcc
     2341 cgacgacggt tcacgccgat cacctgatcc gcgcctatcg tggtgccaac caggacatgc
     2401 aggtcgcgat cgacgagaac aacgaggtgt acgcgttcct gcagtcggct tccgctcgct
     2461 acggaatcgg cttctggcaa cccggcgccg ggatcatcca ccaggtggtg ctcgagaact
     2521 acgcgttccc cggcgggctg atgatcggca ccgattcgca cacgccgaac gccggtggcc
     2581 tcgggatgtt cgcctcgggt gtcggcggcg ccgatgccgt cgacgtgatg gccggattcc
     2641 cgtgggaggt gcgctacccg aacctgatcg gggttcgcct caccgggaag ctcgacggct
     2701 gggcggcacc caaggacgtg atcctcaagc tggccgggat cctgacggtc acgggcggaa
     2761 cgaacgcggt cgtcgagtac ttcggtccgg gtgcggcctc gatctcgtgc accggcaagg
     2821 cgacgatcac gaacatgggc gccgagatcg gcgcgacgac gtcgatcttc ccgttcgacg
     2881 cgtcgatggg gcgttatctg cgcggcaccg agcgagcggc gctcgccgag ctggccgagc
     2941 gccatccgca tctgctccag gcggacgacg acgtcgcccg cgatcccgac aagttcttct
     3001 cgcgcgtgat cgagatcgac ctgtcgtcgc tcgaaccgca cctcgtcgga ccgcacacgc
     3061 cggacctcgc ccggccggtc tccgaggtcg cgcgcgcggt cgaggccgag ggctacccga
     3121 acgacatctc cgtcgcactg atcgggtcgt gcacgaactc gtcgtacgaa gacatctccc
     3181 gcgtggccga cgtcgtcggg caggcgcagg cgcacggaat gcaacgcgcg gcgatcccgt
     3241 tgatggtgac gccggggtcg gagcaggtgc gcgcgacgat cgagcgcgac ggtcagatgg
     3301 cgcggctcga gtcgttcggt gcgacggtac tggccaacgc ctgcggcccg tgcatcggcc
     3361 aatggcagcg ggacgagatc aagcccggtc agcgcaacac gatcgtgacc tcgttcaatc
     3421 gcaacttccc ccgccgaaat gacggcaacg ccgagacgat ggcgttcatc gcgagcccgg
     3481 agatcgtcgc cgcctacgcg ctcgcgggta agctcgagtt cgaccctcgg cgcgacgagg
     3541 ttcgggttcc gggcggggcg gcgttccggc tcagccctcc ggccccggca cccgacctgc
     3601 cggccgacgg cttcgtgcgc gacgcgagcg ggtacgtcga gccgccgcgg gacggcgcct
     3661 cggtcgaggt gcgaatcgcc gcgggcagtc aacgcctgca gcgcctcgcc ccgtttgccg
     3721 actgggacgg gagggatttc gaccgtctgc cgttgctgct caaggtgaaa ggcaagtgca
     3781 ccaccgacca catctcgccc gccgggccct ggttgcgctt ccgcggccac ctggacaaca
     3841 tctcggacaa catgttcacc ggcgccagca acagcttcac ggagaagccg ggcacggcga
     3901 tcaacacgct ggacgaagac acggtgcagc ccgtccccaa ggtcgcgcgg gcctacaagg
     3961 aggccggcat cccctgggtg gtcatcggcg acgacaacta cggggagggc tccagccgcg
     4021 agcacgcggc catggaagtg cgcttcctgg gcgggcgcgc ggtcatcgcc cgatcgttcg
     4081 cgcgtatcgc ggagacgaac ctgaagaagc agggcgtgct ggcgctgacc ttcgacaacc
     4141 cagcggatta cgacaagttc ggcccgcatg accgcgtctc cgtccggggc ttagccagct
     4201 tcgcgccgga acagccgctg cacgtggaga tcgaacaccc ggacggcagc cgcgacacga
     4261 tcaccgtgcg ccactcgtt
//
LOCUS       DASPGN010000016        16832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_939709, whole genome shotgun sequence.
ACCESSION   DASPGN010000016 DASPGN010000000
VERSION     DASPGN010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 16832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16832
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939709"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1006
                     /locus_tag="VD788_00520"
     CDS             131..1006
                     /locus_tag="VD788_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HXV74772.1"
                     /translation="MHGKDHPTHLQHHFDTPLQQFDSAKLGMWLFLATEILLFGGLFC
                     AYAVYRGNHPEIFMWSHHLLDTRLGAINTVILLCSSLTMAWAVRCSQLGQQRGLIILL
                     LLTLSGGFGFLGIKYVEYSSKYKHGLLWGAEFQPDPHYLAEHFGLGPGAVGHHDDATP
                     GGDDPHEAERPAAGEAATTGHDLRDGVTGERSTIAPAAAGPLGRAPATEATAHAMPQP
                     QNVHVFFSIYFLMTGLHGLHVVAGMAVLAWLILGAIRGRFSSDYFTPVDLGGLYWHLV
                     DLIWIFLFPLLYLIH"
     gene            complement(1064..1825)
                     /locus_tag="VD788_00525"
     CDS             complement(1064..1825)
                     /locus_tag="VD788_00525"
                     /inference="COORDINATES: protein motif:HMM:NF033580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HXV74773.1"
                     /translation="MSDDHWERIRHHFPEEHIPTGRPGRKPIPARRVLEGTLWILNTG
                     AQWHMLPQCYPNYKTVHRRFQQWCQSEVLRNVLTDLANTLRDQGGIDESECFIDAMFA
                     SAKGGGNEIGPTKRGKGVKIMGIVDRHGLPLAVSTHAANHHEVTLVQLSFDFYMIEAK
                     PENLIDDRAYDSDKLDRELKKDGIEMIAPHRSNRKMRTQDGRRLRRVQRRWLVERFFA
                     WLQWQRRLLVRWEYYAENFLGFVQLASISILLKRF"
     gene            complement(1901..5788)
                     /locus_tag="VD788_00530"
     CDS             complement(1901..5788)
                     /locus_tag="VD788_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXV74774.1"
                     /translation="MRATTIVLALLLETGLAPVGATDVAIADLDGDGTLDAAVGTPDG
                     VLRLHRGDPGSLFPAAGRAAAPFGPAGSPAHVPIPVDRLLAGDFDADGRTDLRAAARG
                     HAEMFLLRVDRAGNLAGPERVALPGALTAIGIGELDRVDGLADLAVAVVAGDGAELLL
                     YRGAAGALRSAPESAPLPTHVDAIVLGRLDGDSYADVAVLAGSEIRMLSGRSPRAPKP
                     TRTWIPLAPDGAARDMLQLPSSTLRARRVAAARLDRDAVDDLVTVTEDGELAVELSGR
                     ATTFVVTGTADGGAGSLRDAIASANASAGADVIEFAIPGAGPHTITPAIPLPVIAEAV
                     VIDATTQPGYAGTPLVVLDGSGAGFAGLNVTGGSSVIRGLAIGNLSTGIVLSGAGGNF
                     VEACHIGIDPSGTTSMPNTTGVIVSASGDNVVGGTADAAANVISGNSHSGVSVGSAAR
                     VEGNRIGTDVTGTVAVPNDHGVLVLAGGATVGGSTPGSGNLIAGNAGSGVQIQGADDV
                     VVQGNAVGVDATGTTPLGNGEGIGVVSSDRATIGGTAPGTGNQVSGNTTSPSSGIGIH
                     VSDSADTLIRGNRIGTSATGNLPLGNNTGIAVDGASTFTEVGGGLPGSVNVIGGNHVV
                     GVSVSGAHDTRIAGNLIGTTLDGLSALGNGLWGVRVRGVSTGTRIGGITPDDRNVVAG
                     SGEFAIAVEGDSGPPTGTVIEGNFIGTDVTGLASPGAAGSGILLADAPDVRIGGSDPF
                     ARNVIVGHTGCGIVLFGPSSGETVVQNNLVGLAADGLTEIPNLTGVCFNAASGYSIGA
                     AGSGNVISGSNSAGVYVSQDSGGNFVVGNLIGLDASGVLARPNLVGVEVNGPSTTVAQ
                     NTISGNTGSGIWLGGQTADSALVANNTLGETLSGAPAGNLVGIYSFRANNALIANNQV
                     RYNLDGGIVVREAISNQLTANLIADNGGLGIDLDDDGVSPNDPGDVDGGANFLQNFPV
                     LTTVTYCGVQLDTSGTLDSAPSSTYDIELFLSRGCDPSGQGEGDGLYDDVPSLATDAG
                     GSAGFSLSTFRILPAGLPLFASATATGFDGNTSEFSACVPLTDDRPGEVAGVAWPGKT
                     EMTWTPLPGVTGYRVYRGDRATLPALATPESDSSLIWTGGASTTGPSLSDMPAAGGFH
                     WFLVRATGSCGEGSPGSGRPAWRLHDSAGTISSCSHDLCTVGPVLAPDCSPCARQICA
                     ADPYCCDTEWDLQCVAEVQTICGSLTCAANAGACAHPQCTPGVALTAGCDSPPLPSSC
                     VAAVCALDGYCCSTEWDEVCVHEVPLICDGNCD"
     gene            6022..6267
                     /locus_tag="VD788_00535"
     CDS             6022..6267
                     /locus_tag="VD788_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXV74775.1"
                     /translation="MRSTIDAAGRIVIPKEIRDRLGLDGGRVVEIREREGRIEIEAAT
                     TSISLVKRAEGSVAVAEEKLPPLTDEIVRDTLERTRR"
     gene            6264..6668
                     /locus_tag="VD788_00540"
     CDS             6264..6668
                     /locus_tag="VD788_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXV74776.1"
                     /translation="MIAVDTSVVVAAFASWHEGHRAASAVLARHPRVPAQVLIETYSV
                     LTRLPPPHRAPASLVAEFLAERFRSGPITLPASKYRRVIEQAASAGIHGGSVYDAVIA
                     ATARHANARLLTRDRRATPTYDRLGVDYELLS"
     gene            complement(6690..6833)
                     /locus_tag="VD788_00545"
     CDS             complement(6690..6833)
                     /locus_tag="VD788_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74777.1"
                     /translation="MIQSGEAAVFLGGKKHTVLEPGDYFGEISARSCARTASSAGSCS
                     RAS"
     gene            complement(6854..9406)
                     /locus_tag="VD788_00550"
     CDS             complement(6854..9406)
                     /locus_tag="VD788_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV74778.1"
                     /translation="MPLERGLRLGEYEILEPIGAGGMGEVYRARDHRLDRDVAIKVLP
                     EAVAEDAAALARFEREAKAVASLSHPSILAIHELGSAEGRRFAVTELLEGQTLRELLA
                     ERALPARKAADLGRQTARGLAAAHDRGVVHRDLKPENLFVTRDGRIKILDFGLAGRTE
                     DEPPPAEATRTSLTAPGTILGTVGYMSPEQVRGEPVDHRSDIFSFGAVLYEMLTGKRA
                     FARDTKAETMTAILKEEPPEISASGVHVPHTLQTILLRCLEKRADERFQSAHDLAFSL
                     QDTSEAGAPAVAGVARKRPSRLLAAALLLVGLAGGAALATLFRKPPPEPPRFTTLTAR
                     RGAVTSARFVPGARTVYYGAAWDGAPTHVYTISAASLGPRELPFGNAEVLSVSPSGEL
                     ALSLGRHYTVGWEAIGTLARAPGEGSAPRPILEDVLEADWGPDGEELAVVHEVDGVVR
                     LEYPIGKVIYESPGWISSPRTHPDGRRVAFVDHPVRGDNQGEVAIVDREGKVERLGRN
                     GSQGLAWSPDATEVWACRGGQIMGLRPGAAPRSIFAFPGVAYLLDVVPGGNVLMAVAD
                     LRREMRGRARGSPGEINLGWLDWSQLSAFSADGSIAIFEEGNQGTREGYPIYVRSTDG
                     SPPVQVGNGIALAISPDKSRVLAVRSPFTTPQVVIQPTGPGDAITLDTGELRPKSTGV
                     WLPDDSAVVLVAQHPGDVGDRLYELSFEPGSMPHALGEETVMTGRSALAVSPDSRFVA
                     AKTSDGKIKLFPVDGGEPRVVPGAGDADEAPLRFSADGRSLYVAGRSTLPTPILRIDL
                     ATGRRTRVRDLAPGDPAGVFSVDQVQISGDGESYAYSYRRISSRLVLVEGLR"
     gene            complement(9414..12113)
                     /locus_tag="VD788_00555"
     CDS             complement(9414..12113)
                     /locus_tag="VD788_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV74779.1"
                     /translation="MNLAPGTRLLHYRLIDKIGEGGMGAVWRARDTTLEREVAIKVLP
                     EQVAHDGERLSRFDREAKLLAQLNHPNIAAIYGFHRAGEDNLAFLAMELVEGEDLARR
                     LARGPLPVDEALDVFRQIAEALEAAHERGVVHRDLKPANVKLTPDGKVKVLDFGLAKA
                     MSPDTASGGSDVSLSPTMTSDRTRAGVILGTAAYMSPEQARGRSLDRRTDLWSFGCVM
                     HECLTGTQIFRGETVSDSLAAILRKEIDWSELPAGVAPGVRRLVARCLTRDPRKRLQD
                     AGDARVELEQAIEEPSAAGVAVAPPVPLRVVRLVPWLVAVLAVVVAVSLWLRGSQPVS
                     SGASIARRLTIPVPGPTRFGDLAASPPAISPDGRLIVFGVTDKGETKLWMRPLSDFGA
                     RPVAGAEGGQYAFWSPDSKHVGFFRGGRLRRIELATGREQAIGGEGSSYPRGASWNAR
                     GQIVFAPNSNAGIYLIDAAGGEPRPITTPDPAISDSSHRWPWFLPDGEHFLFTLWTND
                     PEARRQHGGVYVGSIAGDEPPRRLVPDASNAAFVPPDHLLLARGDALVAFPFDVTALR
                     VEGDPAVVADDVLGNNSNGYSAFSVSEDGTLVYATGQAVVSSSLRWYDRTGAVTDTPV
                     EPASFRNTRLAPGAGRAATVIPGPTGDGEIWIVDLVRGVRMRLAHGAVTYEYPVWSRD
                     GSRILFAGQIGAGLDLFTRRSDGSGAEEPVLVDGRDKSLYDWSRDGKYAAYWPTGAGG
                     GTPDIWIYSIERQAAVPLITGEPVYEDARFSPDGRWIAYVSDDSGRREVFVQALEGED
                     RTTAGARWQASTAGGVFPHWRDDGRELLYLDLQQRVVAVSIETAGSELEIGAPVELFA
                     LDGVVIDMDATGDHRRLLVAAEESVRTEPLRVVLDWPAEIAGR"
     gene            complement(12345..13025)
                     /locus_tag="VD788_00560"
     CDS             complement(12345..13025)
                     /locus_tag="VD788_00560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV74780.1"
                     /translation="MIVAAPIRVAAVEDDPRFRKTLETLMGHAPGFELCASFASAEEI
                     LREAVRRERDGRIHGWDLVLMDISLGGLNGIRATERLRELDAKLPIVVLTVFEEPNVI
                     VEAIAAGASGYLLKKTSARELLFQLRSVVGGGAPLTPAVASTLLDLVRSGPVARSGVE
                     PARLELTEREQQVLRCLVDGCKYQAAAERLGVSIETVRTHVRGVYAKLQVHSVASAVR
                     EAIRRGLV"
     gene            complement(13022..15928)
                     /locus_tag="VD788_00565"
     CDS             complement(13022..15928)
                     /locus_tag="VD788_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV74781.1"
                     /translation="MRGPRSARLSRATAALCAALIATWTAVPPAVGSERYRRDWSDHP
                     GMPRGIRDLDYDDRGFLWLASEEGIHRFDGQEVVPWSPPGVREVVGINRGPGGRLVAF
                     AEQGAAWEVVASGVEALDHAGGTRLGNVHDADYGPDGMLWVCDDAGLFRRDAVGSWSR
                     VALPPPEGQVPIRVRGAPGGGAFVGTNRGAFVRVGADGAVQLLFAEPSGLVTRIAVKD
                     GRTQAFALRYGTEHGIYLVEDGAARLVWRPASGPRWTGLAFRDDTLWVASTVESAAIL
                     DDGRRVEPLGPEHGFPPGGDLVVDHEKSLWLTSLRGTYQFPEPDVELVTEMFGTREVH
                     RVRSDVVVGRWRGPHRLEADGGWRALGPAGYEIFDVGGVAPWGSTWWVAVREPRTPRH
                     RATLLEIRRDGWTEWVVRDYGVFDGAYATDERGKFWIVFDDTLWRVDRAGAAPRPVAK
                     LALEKAVPTGLVVDGGRARAAFRYQPYCEARLNAAGDALDGAWECETIEGARELLASI
                     EVDGDPWLATWDRGVVRRRGGTWETIVGEEHLGTPTVRGISTSPRGGVWAMSYLDRVR
                     VELRDGRAEVVERLGPWIGVPNWMTFSAREQENGTVWLTGMTSAVRIPAHARARPTDP
                     PPIFATGFRADGRDLAPDAAHELPASTRRIELRWSSPAFRDPASLRFEVRAGESGAWV
                     PTRERSFRFAGLEPGDHRIEVRASLDGKRWSAEPAGFRFSIRSPLRQRPAFWLGLLAV
                     VAVGGLLVQALRARQHLRLERQRTEIAMNLHDELGAGLASIGLLADLAGGELDPAERR
                     EVAGRVGEISRGLSRSLSDIVWTLRPNSTDLAGFALFLRQRAADLLSAGETVVEFDFP
                     EPPPAVRLQLAVRRQIHAIASEALHNAARHAGATRVCVGLSRDGAAWILRVEDDGVGF
                     AGDGGGGRVDSDGLGLESMRRRAQAIGARLTVDSAAGAGCRLELRFRPHVEGRR"
     gene            16253..16600
                     /locus_tag="VD788_00570"
     CDS             16253..16600
                     /locus_tag="VD788_00570"
                     /inference="COORDINATES: protein motif:HMM:NF039191.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
                     /protein_id="HXV74782.1"
                     /translation="MRTVTDEDPVSFDELRRLAAGRFGDLVKAVVDVERRIMLVDADL
                     HADQEAELLAGGSQQQDVWGINLYPDLPESDWLEFDSMINLRPSFGNRSRGVDDPAVR
                     ERITRLVVGLVRR"
     gene            16597..>16832
                     /locus_tag="VD788_00575"
     CDS             16597..>16832
                     /locus_tag="VD788_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74783.1"
                     /translation="MTDAKHRDLTAERWRTFTLAEQLGNVGSEVSRTLKWRSRNPEIA
                     ERAMARALELIDLTLDDPRQRLSVARLRELCRVR"
BASE COUNT         2431 a         6018 c         5947 g         2436 t
ORIGIN      
        1 ggccgcgcgc cggcctgcgc ggctgcgtca tgtactggca cttcctcgac ggcgtctggc
       61 tcgtgctgtt cgggttcttg tggttcaccc gctgaccgcg aacgggtcga gcgaggcata
      121 ggcgatcggc atgcacggca aagatcatcc gacccatctg caacatcact tcgacacccc
      181 gctgcagcag ttcgactcgg ccaagctggg catgtggttg ttcctggcga ccgagatcct
      241 gctattcggc gggctgttct gcgcgtacgc cgtctaccgc ggcaaccacc ccgagatctt
      301 catgtggtct caccacctgc tcgacacgcg gctcggcgcg atcaacaccg tgattctgct
      361 gtgcagcagc ctgacgatgg cctgggccgt ccgctgctca cagctggggc agcagcgtgg
      421 tttgatcatc ttgctgctgc tgacgctgtc gggcggcttc ggcttcctcg gcatcaagta
      481 cgtcgagtac tcgtccaagt acaagcacgg gctgctgtgg ggcgccgagt tccaacccga
      541 cccgcactat ctggccgagc acttcggcct gggtcccggc gccgtcgggc accacgacga
      601 cgcgaccccc ggcggggacg acccgcacga ggccgagcgc ccggccgccg gcgaggcggc
      661 cacgaccggc cacgacctgc gggacggcgt caccggcgag cgctcgacga tcgcgccggc
      721 ggccgccgga ccgctcggcc gcgcccccgc gaccgaggcg accgctcacg cgatgccgca
      781 gccgcagaac gtccacgtct tcttcagcat ctacttcctg atgacgggtc tgcacggcct
      841 gcacgtggtc gccggcatgg cggtcctcgc ctggttgatc ctcggcgcga tccgcggacg
      901 gttctcgagc gattacttta cgccggtcga cctcggcggt ctgtactggc acctggtcga
      961 tctgatctgg atcttcctct tcccgctgtt gtacctgatc cactgatcgc gccggcccgc
     1021 cttgcccgcc ccgccgatcc gagaccggac actagaacct atctcaaaac cgtttgagca
     1081 ggatcgagat tgaagccaac tggacgaatc cgaggaagtt ctcagcgtag tactcccagc
     1141 gaaccaggag tcgtcgctgc cactggagcc aggcgaagaa gcgctcgacc agccagcggc
     1201 gctgaacgcg gcgaaggcgt cgaccgtctt gtgtccgcat cttccgattg gaccgatgcg
     1261 gcgcaatcat ctcgatgcca tccttcttca gttcgcggtc gagcttgtcg ctgtcgtagg
     1321 cgcgatcgtc gatcagattc tcgggcttgg cttcgatcat gtagaagtcg aagctcagtt
     1381 gaaccagcgt cacttcgtgg tgattcgccg catgcgtgct gactgccaag ggaagaccgt
     1441 gtcgatcaac gatgcccatg atcttcacgc ctttgccgcg tttcgtcggt ccgatctcgt
     1501 taccgccacc cttggccgaa gcgaacatcg cgtcgatgaa acactccgac tcgtcgatcc
     1561 cgccttggtc gcgcaacgtg ttggcgagat ccgtcaagac attgcggaga acttcgctct
     1621 gacaccactg ttgaaaccga cgatgcaccg tcttgtagtt cggataacac tgcgggagca
     1681 tatgccactg cgcgccggtg ttcaggatcc acaacgtgcc ttcgagaaca cggcgcgctg
     1741 gaatcggctt ccttcctgga cgacctgtag gaatgtgctc ttcggggaag tgatgccgaa
     1801 tgcgctccca atgatcgtcg ctcaagcgaa acatcgagtc gcatcttacc tcaactggat
     1861 tccgcggcag acagtcgatt gccgggtttt gagataggtt ctagtcgcag ttgccgtcgc
     1921 agatcagcgg gacctcgtgc acgcagacct cgtcccactc cgtggagcag cagtagccgt
     1981 ccagcgcgca gaccgcggca acgcagctcg agggaagcgg cggactgtcg cagcccgcgg
     2041 tcagcgccac ccccggcgtg cactgcggat gcgcgcaggc gccggcgttg gcggcgcagg
     2101 tgaggctgcc gcagatcgtc tgcacttccg ccacgcactg caagtcccac tcggtgtcgc
     2161 agcagtaggg atcggccgcg cagatctgcc gggcgcacgg gctgcagtcg ggagctagca
     2221 cgggaccgac cgtgcagagg tcgtgggagc aggagctgat tgttcccgcg ctgtcgtgga
     2281 ggcgccacgc cggacggccg gatccgggcg acccctcgcc acacgacccg gtcgcgcgca
     2341 cgaggaacca gtggaatcca cccgccgcgg gcatgtccga cagggaggga ccggtcgtgg
     2401 atgcgcctcc ggtccagatc agtgacgagt ccgactccgg ggttgcaagc gcggggagcg
     2461 tggccctgtc gccgcggtac acgcggtagc ccgtgacgcc gggcagcggc gtccacgtca
     2521 tctcggtctt gccgggccac gcgacgccgg cgacctcccc gggccggtcg tcggtcagcg
     2581 gaacgcaggc ggagaactcg gacgtgttgc cgtcgaagcc ggtggccgtg gcgctggcga
     2641 acagcggcag gcccgcgggg aggatccgga aggtcgaaag ggagaagccc gccgacccgc
     2701 cggcgtcggt cgccagcgat ggcacgtcgt cgtagagccc gtcgccctcg ccctggccgc
     2761 tcggatcgca accccggctg aggaacagct cgatgtcgta ggtggaggaa ggtgcgctgt
     2821 cgagggtccc ggaggtgtcg agctgcaccc cgcagtacgt cacggtcgtc agcaccggga
     2881 agttctgcag gaagttggcg ccgccgtcca cgtctcccgg gtcgttcggg ctgaccccgt
     2941 cgtcgtcgag gtcgatgccc aggccgccgt tgtcggcgat gaggttggcg gtcagctggt
     3001 tcgagatcgc ctcccggaca acgatgcctc catcgaggtt atagcggacc tggttgttcg
     3061 cgatcagtgc gttgttggcc cggaaggagt agatccccac gagattcccg gccggtgctc
     3121 ccgagagcgt ctcgcccagg gtgttgttcg cgacgagggc ggagtccgcc gtctgcccgc
     3181 cgagccagat gccgctcccc gtgttgcccg agatggtgtt ctgcgccacg gtggtggagg
     3241 ggccgttgac ctcgacgccg acgaggttcg ggcgggccaa gacgcccgag gcgtcgagcc
     3301 cgatgaggtt gccgacgacg aagttgccgc cgctgtcctg ggagacgtac acgccggcgc
     3361 tgttgctccc cgagatcacg ttgccggatc ccgcggcacc gatactgtag ccggacgccg
     3421 cgttgaagca gacgccggtg agattgggaa tctccgtgag gccgtcggcg gcaagaccga
     3481 ccaggttgtt ctgcacgacc gtctctccgg aggacgggcc gaagaggacg atgccgcagc
     3541 ccgtgtgtcc gacgatcacg ttccgggcga aggggtcgct gccgccgatg cggacgtccg
     3601 gggcgtccgc gagcaggatc ccactcccgg cggcgccggg ggaggcgagg ccggtgacgt
     3661 cggtgccgat gaagttgccc tcgatcaccg tgccggtcgg gggtccgctg tcgccctcga
     3721 cggcgatggc gaactccccg gaaccggcca ccacgttcct gtcgtcgggc gtgattcccc
     3781 cgatgcgggt gccggtgctc acgcctcgga cgcggacgcc ccacagtccg ttcccgaggg
     3841 cggagagtcc gtcgagcgtt gtcccgatca gattgcccgc gatccgcgtg tcgtgggccc
     3901 cgctgacact cacgccgaca acgtggttgc ctccgatcac gttcacgctt ccgggcaggc
     3961 cgcccccgac ctcggtgaac gtgctggcgc cgtccaccgc gatgccggtg ttgttgccga
     4021 ggggcaggtt gccggtcgcg ctggtgccga tgcggttgcc gcggatcagg gtgtcggcag
     4081 aatcgctgac gtggatcccg atgccactgg acggcgaggt agtgttgccg gacacctggt
     4141 tgccggtgcc cggagcggtt ccaccgatcg tggcgcggtc ggaggagaca acgccgatcc
     4201 cctcgccgtt gcccaggggc gtcgtgccgg tcgcgtccac ccccacggcg ttgccctgca
     4261 cgacaacgtc gtccgcgccc tggatctgca caccggagcc ggcgttcccg gcgatgagat
     4321 tcccggaacc gggcgtgctg cctccgacgg tcgcgccgcc ggccagcaca agcacgccgt
     4381 ggtcgttggg gacggccacg gtccccgtga cgtcggtccc gatccggttc ccctcgaccc
     4441 gtgccgccga gccgacgctc acgccggagt ggctgttgcc ggagatgacg ttggccgccg
     4501 cgtccgccgt gcccccgacc acgttgtccc cgctcgccga gacgatgacg ccggtggtgt
     4561 tcggcatcga agtcgttccc gacgggtcga tgccgatgtg gcaggcctcg acgaagttac
     4621 cgccggcgcc ggagagcacg atgcccgtcg agaggttgcc gatggcgagt ccgcggatca
     4681 ccgaggagcc gccggtcacg ttgagaccgg cgaagccggc tccggagccg tccagcacga
     4741 ccaggggcgt gcccgcgtag cccggctgcg tcgtggcgtc gatcactacg gcctccgcga
     4801 tcaccggcag cggtatcgcc ggcgtgatcg tgtgcggtcc ggcgccgggg atggcgaact
     4861 cgatcacgtc ggcgccggcc gaggcgttcg cgctcgcgat ggcgtcccgc agcgatccgg
     4921 cgccgccgtc ggcggtgccg gtaaccacga acgtcgtcgc ccgcccgctc agctccactg
     4981 cgagctcacc gtcctcggtg acggtgacga gatcgtcgac ggcgtcgcga tcgagccgcg
     5041 cggcggcgac acggcgcgcg cgcagggtgc tcgacggaag ctgcagcatg tcgcgggcgg
     5101 cgccgtcggg cgcgagcggt atccacgtgc gcgtcggctt cggtgcacgc ggggagcggc
     5161 ccgacaacat ccggatctcg ctcccggcca gcaccgcgac gtcggcgtag gaatcgccgt
     5221 cgagccggcc gagcacgatc gcgtcgacgt gcgtcggaag cggcgccgac tcgggagcgc
     5281 tgcggagcgc cccagcggct ccgcgataca gcaggagctc ggctccgtct cccgcgacga
     5341 cggcgaccgc gaggtcggcc agtccgtcga cccggtccag ctcgccgatg ccgatggcgg
     5401 tcaacgcgcc cggcagcgcg acgcgctcgg gcccggcgag gttcccggcc ctgtcgacgc
     5461 gcagcaggaa catctcggcg tggccgcggg cagcggcccg caggtccgtc cggccgtcgg
     5521 cgtcgaagtc gcccgccagc agccggtcca ccgggatcgg tacgtgcgcg ggcgacccgg
     5581 cgggaccgaa gggcgctgcg gcccggcccg cggccggaaa gagcgagccc ggatcgccgc
     5641 ggtggagccg gaggactcca tcgggggtgc ccaccgcggc gtcgagcgtc ccgtccccgt
     5701 ccaggtccgc gatcgccacg tcggtcgcgc ccaccggggc aagccccgtt tccagcagga
     5761 gcgccagcac gatcgtcgtc gctctcatgg acctcgcctc caccggaacg ccccggctgt
     5821 ggcgaaagta ccgtcttaga acgcgcggag aaatcccccg atcatgtgag aagctcgggg
     5881 agctttccag aatcaggtct tggagccgcg ggctacgcgg aggggcggtg ccagggtcgc
     5941 tgccgccggc attgctgacc gtcgctcgcc tgggtgcttc gatgcctggc gcgtgggatg
     6001 cttggatggt atttttgtgg catgcgatcc accatcgacg ctgccggccg catcgtcatt
     6061 cccaaggaga tccgggatcg tctgggcctc gacggcggtc gggtcgtcga gatccgcgag
     6121 cgggagggcc gcatcgagat cgaggccgcg acgacctcca tctccctcgt caagcgcgcc
     6181 gaggggtcgg tcgcggtagc cgaggagaaa ctccctcccc tcaccgacga gatcgtccgc
     6241 gacacgctgg aacgtacgcg acggtgatcg ctgtcgacac gagcgtcgtc gtcgcggctt
     6301 tcgcgtcgtg gcatgaaggc catcgagccg cgtcggcggt gctggcgcgt catcctcgag
     6361 tgccggcgca ggtgctgatc gagacctact cggttctgac gcgcctcccc cctccgcacc
     6421 gcgctccggc gagcttggtg gcggaattcc tggccgagcg gttccgaagc gggccgatca
     6481 cgttaccggc gagtaagtac cggcgggtca tcgagcaggc ggcgtccgcc ggcattcacg
     6541 ggggcagcgt ttatgacgcg gtgatcgcgg cgacggcgcg ccatgcgaac gcccggctct
     6601 tgacgcgcga ccgccgagcg acaccgacgt acgatcgcct gggcgtcgac tacgagctcc
     6661 tgagctgacc gcggccggca agccgaggcc taggaagccc tggagcagct tccagccgat
     6721 gatgccgttc gcgcgcacga ccgggcggag atctcgccga agtagtcgcc gggctcgagc
     6781 accgtgtgct tcttgccgcc gaggaacacc gcggcctcgc cggactggat cacctcccct
     6841 cgaggccgtc gcctcagcgc aacccttcca ccaggaccag ccgcgagctg atcctgcggt
     6901 acgagtaggc gtaggactcg ccgtccccgc tgatctgcac ctggtcgacg ctgaacacgc
     6961 ccgcggggtc gcccggcgcg agatcgcgga cgcgggtgcg ccttccggtc gccaggtcga
     7021 tgcgcaggat cggtgtcggc agggtcgagc ggccggcgac atagagcgag cggccgtcgg
     7081 cgctgaagcg cagcggggcc tcgtcggcgt cgcccgcgcc cggaaccacc cgtggctcgc
     7141 ctccgtcgac cgggaacagc ttgatcttgc cgtcgctcgt cttggccgcc acgaagcgcg
     7201 aatcggggga gacggcgagg gccgagcggc cggtcatcac ggtctcctcg ccgagggcgt
     7261 gcggcatgga gccgggctcg aacgaaagct catagagacg atcccccacg tcccccgggt
     7321 gctgggcgac caggaccacg gccgaatcgt cggggagcca gacgccggtg gacttggggc
     7381 gaagctcgcc cgtgtcgagc gtgatcgcgt ctccgggacc ggtcggctgg atcacgacct
     7441 gcggcgtcgt gaacggcgag cgcaccgcga gcacgcggct cttgtcgggc gagatcgcga
     7501 gcgcgattcc gttgccgacc tggaccggcg gcgatccgtc ggtcgatcgc acgtagatcg
     7561 ggtagccctc cctggtgccc tgattgccct cctcgaagat cgcgatcgag ccgtcggccg
     7621 agaacgcgct gagctgactc cagtcgagcc agcccaggtt gatctccccg ggcgagcccc
     7681 gggcacggcc gcgcatctcc cgccgcagat cggcgacggc catcagcacg ttgccgccgg
     7741 gcacgacgtc gaggagatag gccacgcccg ggaaagcgaa gatcgaccgc ggcgcggcac
     7801 cggggcgcag ccccatgatc tggccgcccc gacacgccca gacctcggtc gcgtcggggg
     7861 accacgcgag accttgcgag ccgttgcgac ccaggcgctc gaccttcccc tcgcggtcga
     7921 cgatcgcaac ctcgccctgg ttgtctccgc gcacgggatg gtcgacgaag gcgacgcgac
     7981 ggccgtcggg atgcgtgcgc ggcgagctga tccagccggg tgactcgtag atcaccttgc
     8041 cgatcgggta ctcgagccgc acgaccccgt cgacctcgtg cacgacggcg agctcctcgc
     8101 cgtcgggtcc ccagtcggcc tcgagcacgt cttcgaggat cgggcggggc gccgagccct
     8161 cgcccggggc gcgagccagc gtgccgatcg cctcccaccc gaccgtgtag tgccggccga
     8221 gcgagagtgc gagctcgccc gacggcgaga cggacagcac ctcggcgttt ccgaacggca
     8281 gctcgcgcgg gccgagcgac gccgccgaga tcgtgtacac gtgggtcggc gcgccgtccc
     8341 aggcggcgcc gtagtacacg gtcctcgcgc cgggaacgaa gcgggcactc gtgaccgctc
     8401 cccggcgcgc ggtcagggtc gtgaaccgcg gcggctcggg cggcggcttc cggaaaaggg
     8461 tggcgagagc cgcaccgccg gcgagtccga cgagcagcag cgccgcggcg agcagccgac
     8521 tcggccgctt gcgggccaca ccggcgaccg ccggtgcgcc ggcctccgag gtgtcctgca
     8581 ggctgaacgc caggtcgtgc gccgactgga accgctcgtc cgcgcgcttc tcgaggcagc
     8641 gcaggaggat cgtctggagc gtgtgcggga cgtggacgcc cgaggccgag atctcgggcg
     8701 gctcttcctt gaggatcgcc gtcatcgtct cggccttcgt gtcgcgcgcg aacgcccgct
     8761 tgcccgtgag catctcgtag agcacggcgc cgaaggagaa gatgtcggag cggtggtcga
     8821 caggctcgcc gcgcacctgc tcgggcgaca tgtagccgac ggtgccgagg atcgtgcccg
     8881 gcgccgtcag gctcgtccgg gtcgcctcgg ccggcggcgg ctcgtcttcg gttcgacctg
     8941 cgagaccgaa gtcgaggatc ttgatccgcc cgtcacgcgt gacgaacagg ttctcgggct
     9001 tcaggtcgcg gtgcaccacg ccacggtcgt gcgccgcggc gaggccgcgc gccgtctggc
     9061 ggcccaggtc ggccgccttg cgcgccggca gggcccgctc ggcgagcagc tcacggagcg
     9121 tctgcccctc gagcagctcg gtgacggcga agcggcgtcc ctcggccgac ccgagctcgt
     9181 ggatcgccag gatgctcggg tgcgagagcg acgcgaccgc cttggcctcg cgctcgaagc
     9241 gcgcgagcgc cgccgcatcc tcggccaccg cctccggcag caccttgatc gccacgtcgc
     9301 ggtcgagccg gtggtcgcgc gcgcggtaga cctcgcccat ccccccggcc ccgatcggct
     9361 cgaggatctc gtactcgccc aggcgtaggc cgcgctcgag tggcatgccg ccctcagcgt
     9421 ccggcgatct cggcgggcca gtcgagcacg acgcggagcg gctcggtcct gacgctctcc
     9481 tccgcggcga cgagcaggcg ccggtggtcg cccgtggcgt ccatgtcgat gaccacgccg
     9541 tcgagggcga agagctcgac cggcgcgccg atctcgagct cgctgcctgc ggtctcgatc
     9601 gacaccgcca cgacgcgctg ctggagatcc aggtacagca gctcgcggcc gtcgtcccgc
     9661 cagtgcggga acactccgcc cgcggtcgac gcctgccagc gcgcccccgc cgtcgtccgg
     9721 tcctcgcctt cgagtgcctg aacgaagacc tcgcggcggc cggaatcgtc cgacacgtag
     9781 gcgatccagc gcccgtcggg cgagaagcgg gcgtcctcgt agacgggttc gccggtgatc
     9841 agcggaacgg cggcctgccg ctcgatcgag tagatccaga tgtccggcgt tccgccgcct
     9901 gcaccggtcg gccagtaggc tgcgtacttg ccgtcgcgcg accagtcgta cagagacttg
     9961 tcccgtccgt cgaccagcac gggctcctcg gctcccgagc cgtcggagcg ccgtgtgaag
    10021 aggtcgaggc ccgcaccgat ctgccctgcg aacaggattc ggctgccgtc cctcgaccag
    10081 accgggtatt cgtaggtcac cgcaccgtgt gcgagccgca tccgcacccc gcgcacgaga
    10141 tcgacgatcc agatctcgcc gtcgcccgtc gggcccggga tcaccgtggc ggcgcgtccg
    10201 gctcccggcg ccagcctcgt gtttcggaac gacgcgggct cgaccggcgt gtcggtaacc
    10261 gcgcccgtgc ggtcgtacca ccgcaacgac gacgagacga cggcctggcc cgtggcgtag
    10321 acgagcgtcc cgtcctccga gaccgagaac gcgctgtatc cgttcgagtt gttccccagc
    10381 acgtcgtcgg ccacgaccgc cggatcgccc tcgacccgaa gcgcggtgac gtcgaacgga
    10441 aaagcgacga gggcatcgcc ccgcgccagg aggaggtgat cgggcgggac gaacgccgcg
    10501 ttcgacgcat cgggcacgag gcgccgcggc ggctcgtccc ccgcgatcga accgacgtag
    10561 acgccgccgt gctgccggcg cgcctcgggg tcgttggtcc acagcgtgaa caggaagtgc
    10621 tcgccgtcgg gcaggaacca cggccagcgg tggctgctgt cggagatcgc gggatcgggc
    10681 gtcgtgatcg ggcgcggctc acccccggcg gcgtcgatca gatagatgcc ggcgttcgag
    10741 ttgggcgcga agacgatctg cccgcgcgcg ttccacgacg cgccgcgcgg gtaggacgag
    10801 ccctcgccgc cgatcgcctg ctcgcgcccg gtcgccagct cgatgcgccg gagccggccc
    10861 ccgcggaaga aaccgacgtg cttgctgtcg ggagaccaga aggcgtactg cccaccctcg
    10921 gctccggcga ccggtcgcgc tccgaagtcg ctcagcggcc gcatccacag tttcgtctcg
    10981 cccttgtcgg tcacaccgaa cacgatgagg cgaccgtcgg gcgagatcgc cggcggcgac
    11041 gcggccagat cgccgaaccg ggtcggaccg ggcacgggga tggtgagacg gcgcgcgatc
    11101 gatgcgccgg aggagaccgg ctggctgcca cggagccaca gagaaactgc gacgacgacg
    11161 gccaggacgg cgacgagcca gggtacgaga cgaacgacgc ggagcggaac gggcggagca
    11221 acggcgacgc cggcggcaga aggctcctcg atcgcctgct cgagctcgac gcgcgcatcg
    11281 cccgcgtcct gcaggcgctt acgcgggtcg cgcgtcaggc agcgggcgac cagccggcgc
    11341 acgcccggcg ctacaccggc gggcagctcc gaccagtcga tctccttgcg cagaatcgcg
    11401 gccagcgaat ccgacaccgt ctccccgcgg aagatctgcg tgccggtcag gcactcgtgc
    11461 atcacgcagc cgaaggacca gagatcggtg cgccggtcga gcgaccggcc gcgcgcctgc
    11521 tcgggcgaca tgtacgcagc ggtcccgagg atcaccccgg cgcgcgtgcg gtcgctggtc
    11581 atcgtcggcg acagggacac gtcgctgccg cccgacgcgg tgtcgggcga catcgccttg
    11641 gccaggccga agtcgagcac cttcaccttg ccatcgggcg tcagcttgac gttcgccggc
    11701 ttgaggtcgc ggtgcacgac gccgcgctcg tgcgcggcct cgagggcttc cgcgatctgg
    11761 cggaagacgt cgagcgcctc gtcgaccgga agcggccccc gtgcgagccg ccgggccagg
    11821 tcctcgccct cgacgagctc catcgccagg aacgcgaggt tgtcctcgcc tgcgcggtgg
    11881 aacccgtaga tcgcggcgat gttggggtgg ttgagctgcg ccagcagctt ggcctcgcga
    11941 tcgaagcgcg agagccgctc gccgtcgtgg gcgacctgct cgggcagcac cttgatcgcg
    12001 acctcgcgct cgagcgtcgt gtcgcgcgcg cgccacaccg cgcccatgcc cccctcgccg
    12061 atcttgtcga tcaggcggta gtgcagcagc cgtgttccag gtgcgagatt catggtcggg
    12121 cggagtttag cgcggggggc cggttgaagc tattgaagga ctcatccgtg atcgccggcg
    12181 acggcctgct gggcggcgga gcagggtgca cgggccgaga cgttcgccct cctgcgcgaa
    12241 tccgctgcta ggctggacga atcgtgaagc gctccagcgg tcctcgctgc ggacgacgag
    12301 cgaggcggcg cagtctctcg gtcctgcagg atcgaggtag ggcgtcagac gagcccgcgg
    12361 cggatcgcct cgcgcaccgc ggacgcgacg gagtgcacct gcagcttcgc gtagacgccg
    12421 cgcacgtgcg tgcgcacggt ctcgatgctg acgccgagcc gctccgccgc cgcctggtac
    12481 ttgcaaccgt cgaccaggca ccgcaggacc tgctgctcgc gctcggtgag ttcgaggcgc
    12541 gccggctcga cgccggagcg ggcgaccggc cccgagcgca cgaggtcgag cagggtgctc
    12601 gccaccgccg gcgtcagcgg ggcaccgccg ccgacgacgc tgcgcagctg gaacagcagc
    12661 tcgcgcgcgc tggtcttctt cagcaggtag ccgctggcgc cggcggcgat cgcctcgacg
    12721 atcacgttcg gctcttcgaa gacggtcagc acgacgatcg gcagcttcgc gtcaagctcg
    12781 cgcagacgct ccgtcgcgcg gatgccgttc agcccgccca ggctgatgtc catcagcacc
    12841 agatcccagc cgtggatgcg gccgtcgcgc tcgcggcgca cggcctcgcg caggatctcc
    12901 tccgccgagg cgaagctggc gcacagctcg aaacccggcg cgtgccccat cagcgtctcg
    12961 agcgtcttcc ggaagcgggg gtcgtcctcg acggcggcga cccggatcgg tgctgcgacg
    13021 atcatcggcg gccctccacg tgcggtcgga agcgcagctc cagccggcag cccgcgccgg
    13081 ctgcggagtc gactgtcagg cgggcgccga tcgcctgggc gcgccggcgc atgctctcca
    13141 ggccgagccc gtcggaatcg acgcgtccgc cgccgccgtc tcccgcgaac ccgaccccgt
    13201 cgtcctcgac gcgcaggatc cacgccgcgc cgtcgcggct cagcccgacg cacacgcgcg
    13261 tcgccccggc gtgccgggcc gcgttgtgca gcgcctccga ggcgatcgcg tggatctggc
    13321 ggcgcaccgc gagctgcagg cgcaccgccg gcggcggctc agggaagtcg aactcgacga
    13381 cggtttctcc cgcgctcaac aggtcggccg cgcgctggcg caggaacagc gcgaatcccg
    13441 cgaggtccgt cgagttcgga cgcagcgtcc agacgatgtc ggagagcgag cgagagagcc
    13501 cgcgcgagat ctcgccgacg cgccccgcga cctcgcggcg ctccgcgggg tccagctccc
    13561 cgcccgccag gtcggcgagg agaccgatgc tggcgagacc cgcccccagc tcgtcgtgga
    13621 ggttcatcgc gatctccgtt cgttgtcgct ccagccggag atgctgccgg gcgcgcagcg
    13681 cctgcacgag cagcccgccg accgcgacga cggcgagcag acccagccag aacgcgggcc
    13741 gctgccggag gggggagcgg atcgagaacc ggaagcccgc cggctccgcg gaccagcgct
    13801 tgccgtcgag cgacgcgcgc acctcgatgc ggtggtcgcc cggctcgagg ccggcgaagc
    13861 ggaacgagcg ctcgcgggtc ggcacccacg cgccgctctc gccggcgcgg acctcgaagc
    13921 gcagcgacgc cgggtcgcgg aacgccggcg acgaccagcg cagctcgatg cgacgcgtcg
    13981 aggcgggcag ctcgtgtgcg gcgtcggggg ccaggtcgcg accgtcggcg cggaacccgg
    14041 tggcgaagat cggcggcgga tcggtcggtc gcgctcgcgc gtgtgcgggg atgcgcaccg
    14101 ccgaggtcat gccggtcagc cagacggtgc cgttctcctg ctcgcgcgcc gagaacgtca
    14161 tccagttggg cacgccgatc cacggcccca gccgctcgac cacctcggcg cgcccgtcgc
    14221 gcagctcgac gcgcacgcgg tcgaggtagc tcatcgccca gacgccgccg cgcggcgagg
    14281 tcgagatgcc gcgcaccgtg ggcgtgccga ggtgctcctc gcccacgatc gtctcccacg
    14341 tgccgccgcg gcgccgcacg actccgcggt cccacgtcgc cagccaggga tcgccgtcga
    14401 cctcgatgga ggcgaggagc tcgcgcgcgc cctcgatcgt ctcgcactcc caggcgccgt
    14461 cgagggcgtc gcccgcggcg ttcagccgcg cctcgcagta gggctggtac cggaacgccg
    14521 cgcgggccct cccgccgtcg acgacgagcc cggtcggcac ggccttctcc agcgcgagct
    14581 tcgccacggg ccgcggcgcc gcgcccgcgc ggtcgacgcg ccacagcgta tcgtcgaaga
    14641 cgatccagaa tttcccgcgc tcgtcggtcg cgtaggcgcc gtcgaagacg ccgtagtcgc
    14701 ggacgaccca ctcggtccag ccgtcgcgac ggatctcgag cagcgtggcc cggtgccgcg
    14761 gcgtgcgcgg ctcccggacg gcgacccacc acgtcgagcc ccacggcgcg acgcccccga
    14821 cgtcgaagat ctcgtagccc gcgggcccca gcgcacgcca gccgccgtcg gcttccagtc
    14881 ggtgcggtcc ccgccagcgc ccgaccacga cgtcagagcg gacgcggtgc acctcgcgcg
    14941 tgccgaacat ctcggtcacc agctcgacgt cgggctcggg aaactggtag gtcccgcgaa
    15001 ggctcgtcag ccagaggctc ttctcgtgat cgacgacgag gtcgccgccg ggcgggaacc
    15061 cgtgctccgg ccccagcggc tcgacgcgcc ggccgtcgtc gagaatcgcc gcggactcga
    15121 ccgtcgaggc gacccacagg gtgtcgtctc ggaaggccag acccgtccag cgcggcccgg
    15181 acgcggggcg ccaaacgagc cgcgccgcgc cgtcctcaac caggtagatt ccgtgctccg
    15241 tcccgtagcg cagcgcgaac gcctgcgtgc gcccgtcctt gaccgcgatg cgtgtgacga
    15301 ggcccgacgg ctcggcgaac aggagctgca ccgcgccgtc cgcgccgacg cggacgaagg
    15361 cgccgcggtt cgtgccgacg aacgcgccgc cgccgggagc gccgcgcacg cggatcggga
    15421 cctggccctc gggcggcggg agcgccacgc ggctccagct ccccacggcg tcgcggcgga
    15481 agaggcccgc gtcgtcgcag acccagagca ttccgtcggg cccgtagtcc gcgtcgtgga
    15541 cgttgccgag tcgcgtgccg ccggcgtggt ccagcgcctc gacgcccgac gcgacgactt
    15601 cccaggctgc accctgctcg gcgaaggcga cgaggcggcc gccgggcccg cggttgatgc
    15661 cgacgacctc gcgcaccccg ggcgggctcc agggcacgac ctcctgcccg tcgaagcggt
    15721 ggatgccctc ctcgctcgcc agccagagga acccccggtc gtcgtagtcg aggtcgcgga
    15781 tcccccgcgg catcccgggg tggtccgacc agtcgcgccg gtaccgctcg ctgccgacgg
    15841 ccggcggcac cgcggtccac gtcgcgatca gcgcagcgca cagcgcggcg gtcgcgcggg
    15901 aaagccgggc gctgcgcgga cctcgcatgc cggaagcata cgggagtccg tgccccggcg
    15961 agccccccgg ggggcgcttc acacgatcat gcgaggtcgc gaatcgcggg tctgcgtacg
    16021 cgccccgggt cgaaaccggg acgctgccat gctcgcacac cgcacatcgc tcctcgcttg
    16081 tctctgcgcc ggtctcgtcg cgtggatcgt gccctcggcg ccgctgtcgc cgctgttctc
    16141 gcccgtcggg cgggaacgcc gaccggccgc tgctcgtccg ggggttgccg gtggggcacg
    16201 gcgcagcagc cgggtcgcct cagcccatcg gccatgttac ggttcccttc cgatgcgaac
    16261 cgtgaccgac gaagatcccg tttccttcga cgaactgcgg cgcctggccg ccggacgctt
    16321 cggcgatctg gtcaaggccg tggtcgacgt cgagcgccgg atcatgctcg tcgacgcgga
    16381 cctgcatgcc gaccaagagg ccgagcttct cgccgggggg tcgcaacagc aggatgtgtg
    16441 ggggatcaac ctctatccgg acctcccgga aagcgactgg ctcgagttcg attcgatgat
    16501 caacctccgc ccctccttcg gcaatcggtc ccgtggcgtc gacgatcctg ccgtccgcga
    16561 gaggatcacg cgcctggtgg tcgggctggt gcgccggtga ccgacgcaaa gcatcgggat
    16621 ctcacggcgg agcgctggcg gacgttcacg ctggccgagc agctgggaaa cgtcggcagc
    16681 gaggtcagca ggacgctgaa gtggagatct cgtaatcctg aaatcgccga gcgcgcgatg
    16741 gcgcgcgccc tggagttgat cgacctgacg ctcgatgatc cccgccagcg cttgtcggtt
    16801 gctcgactga gggagctctg ccgggtccgg ga
//
LOCUS       DASPGN010000017         3516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1525372, whole genome shotgun sequence.
ACCESSION   DASPGN010000017 DASPGN010000000
VERSION     DASPGN010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525372"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VD788_00580"
     CDS             <1..489
                     /locus_tag="VD788_00580"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXV74784.1"
                     /translation="RVARTNAGGEFFVGNLRPGIYHVELSPDNLPIELAPQLRSLNAE
                     VSGAAVTRVDFIVRPEFGIAGRVRDASGAPVSGMHVEILDGSSAVVVTAVTDRFGLFR
                     VDGLPIGSYLLRPRPRDLAGLDAGAVERVVRITDDFLFGQDLTLPVVVESATTDSDPR
                     SG"
     gene            complement(509..3193)
                     /locus_tag="VD788_00585"
     CDS             complement(509..3193)
                     /locus_tag="VD788_00585"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HXV74785.1"
                     /translation="MRLKIVCSVALATLLFGVQTPAAKKDDKPAEAAKSEKAEGGKPA
                     GEGEGEGAGKKKGEKSFAEVVEDFDKIEGLFTFYRDDKTGKVLMAIEPEQLDRTYMMS
                     ITRAAGDGYFFDSGAMLSDFPFSMKRVGSTVQFLHDNVYFRADEAAAIHRAIPRGLSD
                     SLVGAAKIEGEPHPDSGAVLVDPTGVFVQDIAAVGYIFKEYIKPHAYSFDKANSYFET
                     VRSFPENSEIDVVARFESSAPKNVPTLPDSRSFRHVYHFSLSELPQTSFEPRLADDRV
                     GHFLTMHQDYTSLLEETPYVRYVNRWHLEKAEPKFDRSKPRQPIVYWLENTIPVEYRA
                     AVEQGVLVWNDAFEKVGFKDAIVVKQMPDDADWDPADVRYNVVRWIVQPGGGYAVGPS
                     RTNPFTGQIYDADIRISADMVRSVWAGYERLAQPVAMTIGDRIALDLGLMAGPADGLC
                     DYQVGLRDQAAFGYSVIAARVGADQPVDAERYVNDFLIHVVAHEVGHTLGLRHNFKAS
                     TIHDNDALQTTTAAERGLTGSVMDYSPVNIAPDGVGQGEYWQTRLGPYDYWAIEYAYR
                     PYDPDGGVSEKAMLDQIASKVADPMLAYATDEDTMGGGRGMDPSSNRWDLGDDPISYY
                     TGRVAIARELWDKLEGKFEQPGERYNKLRQVFAQGVNQYFIAVLNVPKYVGGVYQYRD
                     HVGDPGGRLPLQVVPAAEQREALRFLTEEIFSTTAFEFPPTLFEKLAPERFWDFAGSL
                     FEQQRLDYPVHDVVAAIQGVPLDHLYNGLVMSRIVDSELRASNGEDPLTLAEMFDEVR
                     RAIWSELGDASDVDSFRRSLQRAHLQKLIALVVNPPNGTPEDATTLARADLRALTASI
                     GRSLNNTDLDPTTRAHLDETAARIDAALAASIERQLKM"
BASE COUNT          544 a         1169 c         1170 g          633 t
ORIGIN      
        1 agggtggctc ggacgaacgc gggtggcgag ttcttcgtcg gcaacctgcg accggggatc
       61 tatcacgtcg agctgagccc ggacaacctg ccgatcgagc tcgcgccgca gctgcgctcg
      121 ctgaacgcgg aggtatcggg agcggcggtg acccgcgtcg atttcatcgt gcgacccgag
      181 ttcggcatcg ccggccgcgt tcgtgacgcg agcggggcac cggtctccgg gatgcacgtc
      241 gagatcctcg acggttcgtc ggcggtcgtc gtgacggcgg tgaccgaccg cttcggtctg
      301 ttccgcgtcg acggcctgcc gatcggcagc tacctgttgc gcccgcgtcc ccgcgacctg
      361 gccggactcg atgccggcgc ggtcgaacgc gtggtgcgca tcaccgacga cttcctgttc
      421 ggccaggacc tgacgctgcc cgtggtcgtc gaatcggcga ccacggacag cgatccgaga
      481 tcgggctagt ccccggccgg tccgcgcttc acatcttcag ctgacgctcg atcgacgccg
      541 cgagcgccgc gtcgatacgc gccgccgtct cgtcgaggtg cgcgcgggtg gtcggatcga
      601 gatcggtgtt gttcagcgat cgcccgatcg acgcggtcag cgcgcgcagg tcggcgcggg
      661 ccagggtcgt cgcgtcctcg ggcgttccgt tgggaggatt gacgaccagt gcgatcagct
      721 tctgcaggtg ggcgcgctgc agcgagcggc ggaagctgtc gacgtcggac gcatcaccga
      781 gctccgacca gatcgcgcgg cggacctcgt cgaacatctc ggccagcgtc aacggatctt
      841 cgccgttcga ggcgcgcagc tcggagtcga cgatgcggct catcaccaga ccgttgtaca
      901 ggtggtcgag cggcaccccc tggatcgcgg cgacgacgtc gtggaccgga tagtccagtc
      961 gttgctgctc gaacaacgat ccggcgaaat cccagaaccg ctcgggtgcg agcttctcga
     1021 acagggtcgg cgggaactcg aacgccgtgg tcgagaagat ctcctcggtc aggaagcgca
     1081 gcgcctcgcg ttgctcggcg gcggggacga cctgtaacgg cagccggccc cccggatcgc
     1141 cgacgtggtc ccgatactgg tacactccgc cgacgtactt cggcacgttc aggacggcga
     1201 tgaagtactg gttcaccccc tgcgcgaaga cctgccgcag cttgttgtag cgctcgcccg
     1261 gttgctcgaa cttcccttcc agcttgtccc acagctcgcg ggcgatcgcc acgcgtccgg
     1321 tgtaatacga gatcggatcg tcaccgagat cccagcggtt ggacgacgga tccatccccc
     1381 gcccaccgcc catcgtgtct tcgtcggtgg cgtaggccaa catcgggtcg gcgaccttcg
     1441 acgcgatctg gtcgagcatc gccttctcgc tgacgccccc gtcggggtcg taaggccggt
     1501 aggcgtactc gatcgcccag tagtcgtacg ggccgagtcg ggtctgccaa tactcgccct
     1561 gccccacgcc gtccggcgcg atgttcaccg gcgagtagtc catcaccgat ccggtcaggc
     1621 cacgctcggc cgcggtcgtc gtctgcagcg catcgttgtc gtggatcgtg ctggccttga
     1681 agttgtgccg caggcccagc gtgtgaccga cttcgtgagc cacgacgtgg atcaggaagt
     1741 cgttgacgta ccgttccgcg tcgaccggct ggtcggcgcc gacgcgagcg gcgatcacgc
     1801 tgtagccgaa cgcggcctga tcgcgcaatc cgacctggta gtcgcagagc ccgtcggcgg
     1861 ggccggccat cagtccgaga tccagcgcga tgcggtcgcc gatcgtcatt gcgacgggtt
     1921 gagcgagccg ctcgtagccg gcccagacgc tgcggaccat gtccgcgctg atgcggatat
     1981 ccgcgtcgta gatctggccg gtgaacggat tggttcgcga cggtccgacg gcgtacccgc
     2041 cgccgggctg gacgatccat cgcacgacgt tgtagcgcac gtcggccgga tcccagtcgg
     2101 cgtcgtccgg catctgcttg acgacgatcg cgtccttgaa gccgaccttc tcgaacgcgt
     2161 cgttccagac gagcacgccc tgctcgaccg ccgcccggta ctcgaccggg atggtgttct
     2221 ccagccagta gacgatcggt tgccgcggct tcgacctgtc gaacttcggc tcggcttttt
     2281 cgaggtgcca gcggttgacg taccgcacgt acggtgtctc ctcgagcagc gacgtgtagt
     2341 cctggtgcat cgtcaggaag tggccgacgc ggtcgtcggc cagacgaggc tcgaagctgg
     2401 tctgcggtag ctcggacagg ctgaagtgat agacgtggcg aaagctgcgc gaatcgggca
     2461 gcgtcggcac gttcttcggc gccgagctct cgaaccgcgc tacgacgtcg atctcgctgt
     2521 tttccgggaa cgagcggacc gtctcgaagt agctgttggc cttgtcgaag ctgtaggcgt
     2581 gcggcttgat gtactccttg aaaatgtagc ccacggcggc gatgtcctga acgaagacgc
     2641 cggtcggatc gacgaggacc gcgccgctgt cgggatgcgg ctcgccctcg atcttcgccg
     2701 cgccgacgag cgagtcggac aggccgcgag ggatggcgcg gtggatcgcc gccgcctcgt
     2761 cggcacggaa atagacgttg tcgtgcagga actgcacggt gctgccgacg cgcttcatcg
     2821 agaacggaaa gtcgctgagc atcgcgccgg agtcgaagaa gtaaccgtcg cccgcggcgc
     2881 gcgtgatcga catcatgtag gtcctgtcga gctgttcggg ttcgatcgcc atcaagacct
     2941 tgccggtctt gtcgtcgcga tagaaagtga acagcccctc gatcttgtcg aagtcctcga
     3001 cgacctcggc gaagctcttc tcgcctttct tcttgccggc tccttcgccc tcgccctcac
     3061 ccgcgggctt gccgccctcg gccttctcgc tcttggccgc ctccgcgggc ttgtcgtcct
     3121 tcttggccgc cggcgtctgg actccgaaca acagcgtcgc caacgcgacc gaacacacga
     3181 tcttcaaacg catgggcctg cctccttccg cacgcgagtg gttcccagcc agtgtccggg
     3241 ggccggcggc gccggccagc cgatccgcgc aagatcagca gtctacacgc tcttatacgc
     3301 tccgcccccg gtcgagtttg acggcgaccg gcgattggca ttccgagcga acggaaccga
     3361 cgttggagga gcgcacccgg attcgctaga ctgacgatcc gcgggtcgga cgggccgagg
     3421 gggttcagga caccgatgca ggagtccgaa aaactgcggt tctcgacggt gcccgatccg
     3481 cccaagccgg gtgccgaacc gaccttcggc gattac
//
LOCUS       DASPGN010000018         4930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_3396, whole genome shotgun sequence.
ACCESSION   DASPGN010000018 DASPGN010000000
VERSION     DASPGN010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3396"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4930)
                     /locus_tag="VD788_00590"
     CDS             complement(<1..>4930)
                     /locus_tag="VD788_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74786.1"
                     /translation="VDFLGIVASPDTTLTIEWSDMSDPDTLQVILPPNLFQLPDFTNM
                     DAGTLVSLLGSISNWLEDFRRSFDAEDIPLIGPALDEALRFADLFSDTLLFDDHDDDD
                     DATGTSKLLDAQNRPTFDTVQELVVRLTEIFGAGDYLTYDELAQTLTFALEIGDKDSA
                     TNFGVLDVPIQFDLLQALAPVAELSSDSQIQLSAGGGITLNIGVYLGDEGGTPLANDT
                     LLSSLKGGIAFSQARVVAAPNEVTTTYGRLSGNAKFKLSVDGGDEEEIVIDNDDTEDN
                     SSVDDLVEDINDALALAGLDAQVLAERAEDEDGNPINKINLKALGGVSSLVLTAQQGD
                     PAITQIGFRASMTAQPDADDGDQLKIKGQGDVSGLMGRLSGDAVMQVALDTVNGGAPV
                     IVTVDADDTSTNRNILDVIVDVQRALDDAGFEEEIEVTSLGKNLIFRTLNPAATSLSI
                     TVAGGNPAQQLGLGTSNIGNSHDLVITTSDGVHHAITFGAEVDTLGEVLSEISSQTGG
                     AVTGSFSDGDTRILLTDNLRQTTAVFKVNNAVGSTAAFDLAIFGVDTVEPEPEEDPRD
                     FRIEGGQLGGVDPLDRLFIQNAQAQATAAIVTPQRNANGEIEDTDGDGSTTDGLDAEA
                     RFGFVGIHGHGGISNLAGDGPLTVSVDVGLKPIGAPAFDPQAKITLKDLIENVSDIGS
                     FLDGPNLDGSGKLELAVSVTPSFSEIETGAEPTLTIELTSLAEILNGDDDGDAGYTIT
                     TEGFEDLLNFDDIGFSDILAALRALVDFLEQFEEFGFLNEDIPLINVSINDMLSFAED
                     FEAALDEVESNPAGTVQVLETKLKEAFGIPQSSNLLELALVDGSILRIDLNFEPSFSE
                     SLPVSLELPVDTGPIDLSGAANLRAEGELDLQLAVGIDLNNLANFWVFEETGIAGSLS
                     ASADDISFTAALGGIGARIVDGQAEITGDFNLGLDLDPLAGLTAGSGADRRILLTDLL
                     GNFGEAMDASLTGGVTALLPVYFPTESSFRGNLEVGGALALTPEDGLQVSGTLTGEDV
                     VADGFLRIPDDIFSIDFSQFSALDNLLLIIDGVDGFLGLLQDLFDGEVAGFTLPLVGD
                     QLADAADVIEDFRKDFIDGLRDAVETAADPDENYISNALFDLLHNDLHILADRNEDGA
                     ITVADIQLQTNVDEAGVEPEDVFMQWNLVLGGTLLDAGAGLDFDIGIPGLGLETRGEI
                     NVAVDWELALGFGVGISRGGFYIDTSDVNELELNVDVTLPDAGLTGRLLFLQLDADNN
                     GPTGLGATFGVDVFRQGGGNPLLGIAELGELGVDIGVAVDAIIDLGLELKLNSDLVPG
                     SGKVFPKIVGDFYLEFAIGDRGAGELVGFDEIGNAFEEGLKVVEFRDIGLDLGSFISD
                     FLGPIVEEVQKFTEPFQPVIDVVTAPIPVVSDLAGRDISLVDLAGMTGYVNPDLIYAI
                     ADVITLINSIPEIDPNDPALILPFGDFTIYDIAGGPGSTPSLWDPGFSQEDRDAIALP
                     DINADDIAGALDGLADGAPPSSQQAASFTNGLADKDFGDFISFPIFQDPSQIFGLLMG
                     NDISIIEIDLPEFSFEFTYSQFFPIYGPLGVALGVSAGIVIDPPAFGYDTLGLRNFFD
                     SGFRDPLALFDGLFLSSLEPIVELTASISASA"
BASE COUNT          755 a         1575 c         1748 g          852 t
ORIGIN      
        1 tcggccgatg ccgaaatgct ggcggtgagc tcgacgatcg gctccagcga gctcaggaac
       61 aggccgtcga acagcgcgag cgggtcgcgg aagccgctgt cgaagaagtt gcgcaggccg
      121 agcgtgtcgt agccgaaggc cggcgggtcg atgacgatgc ccgccgagac gcccagggcg
      181 acgccaagcg ggccgtagat cgggaagaac tgcgagtagg tgaactcgaa cgagaactcg
      241 ggcaggtcga tctcgatgat cgagatgtcg ttgcccatca gcaggccgaa gatctggctc
      301 gggtcctgga agatcgggaa gctgatgaag tcgccgaagt ccttgtcggc gagcccgttg
      361 gtgaacgagg ccgcctgctg gctggacggg ggagcgccgt ccgccaggcc gtcgagcgcg
      421 ccggcgatgt cgtcggcgtt gatgtccggc agagcgatcg cgtcgcggtc ctcctggctg
      481 aagcccgggt cccacaggct cggcgtgctg cccggcccgc cggctatgtc gtagatggtg
      541 aagtcgccga acggcaggat cagcgccggg tcgttcgggt cgatctcggg gatgctgttg
      601 atcagcgtga tgacgtcggc gatggcgtag atcaggtccg ggttgacgta cccggtcatg
      661 ccggcgaggt cgaccaggga gatgtcgcgc ccggccagat cggacaccac cgggatcggc
      721 gccgtcacga cgtcgatcac cggctggaac ggctcggtga acttctgcac ctcctcgacg
      781 atcggcccga ggaagtcgga gatgaagcta ccgaggtcca ggccgatgtc gcggaactcg
      841 acgaccttca ggccctcctc gaacgcgttg ccgatctcgt cgaagccgac cagctcgccc
      901 gcgccgcggt cgccgatggc gaactcgagg tagaagtcgc cgacgatctt cgggaacacc
      961 ttgcccgagc ccggcaccag gtcgctgttg agcttgagct cgagcccgag gtcgatgatc
     1021 gcgtcgaccg cgacgccgat gtccacgccg agttcgccga gctcggcgat gcccagcagc
     1081 gggttgccgc cgccttgccg gaaaacgtcg accccgaacg tcgcgcccag gccggtcggg
     1141 ccgttgttgt cggcgtcgag ctgcaggaac agcagcctgc cggtcaggcc ggcgtcgggc
     1201 agcgtgacgt cgacgttgag ctcgagctcg ttcacgtcgg aggtgtcgat gtagaagccg
     1261 ccgcgcgaga tgcccacgcc gaagcccagc gccagctccc agtcgaccgc gacgttgatc
     1321 tcgccacgcg tctccaggcc gaggcccggg atgccgatgt cgaagtcgag ccccgccccc
     1381 gcgtcgagca gcgtgccgcc gagcacgagg ttccactgca tgaagacgtc ttcgggctcg
     1441 acgccggcct cgtcgacgtt ggtctgcagc tggatgtcgg cgacggtgat ggcgccgtcc
     1501 tcgttgcggt ccgcgaggat gtgcaggtcg ttgtgcagca ggtcgaacag tgcgttggag
     1561 atgtagttct cgtccggatc ggccgccgtc tccaccgcgt cgcgcaggcc gtcgatgaaa
     1621 tccttgcgga agtcctcgat cacgtcggcc gcgtcggcca gctggtcgcc gaccagcggc
     1681 agcgtgaagc cggccacctc gccgtcgaac aggtcctgca gcaggccgag gaagccgtcg
     1741 acgccgtcga tgatcagcag caggttgtcg agcgcgctga actggctgaa atcaatgctg
     1801 aagatgtcgt ccggaatgcg caggaagccg tcggcgacca cgtcctcgcc ggtgagcgtg
     1861 ccgctgacct gcaggccgtc ttcaggggtg agcgcgagcg cgccgccgac ctcgaggttg
     1921 ccgcggaagc tcgactcggt cgggaagtac accggcagca gggcggtcac cccgccggtg
     1981 aggctggcgt ccatcgcctc gccgaagttg cccagcaggt cggtgagcag gatgcgccgg
     2041 tccgcgccgc tgcccgccgt cagcccggcg agcggatcga gatccaggcc gaggttgaag
     2101 tcgccggtga tctcggcttg gccgtcgacg atgcgcgcgc cgatgccgcc gagcgccgcg
     2161 gtgaacgaga tgtcgtcggc cgaggcgctc aggctgccgg cgatgccggt ctcctcgaac
     2221 acccagaaat tcgccaggtt gttgaggtcg atgcccaccg cgagctgcag gtcgagctcg
     2281 ccctcggcgc gcaggttggc ggcgccggac aggtcgatcg gcccggtgtc gaccggcagc
     2341 tcgaggctga ccggcagcga ctcggagaac gagggctcga aattcagatc gatgcgcagg
     2401 atgctgccgt cgaccagggc gagctcgagc aggttgctcg actgcgggat gccgaaggcc
     2461 tccttgagct tggtctccag cacctgcacc gtcccggcgg ggttgctctc gacttcgtcg
     2521 agcgcggcct cgaagtcctc ggcgaacgac agcatgtcgt tgatgctgac gttgatgagc
     2581 gggatgtcct cgttgaggaa gccgaactcc tcgaactgct cgaggaagtc gaccagcgcg
     2641 cgcagcgcgg cgaggatgtc gctgaagccg atgtcgtcga agttgaggag gtcctcgaag
     2701 ccctccgtgg tgatggtgta gcccgcgtcg ccatcgtcgt cgccgttgag gatctcggcc
     2761 aggctggtca gctcgatcgt cagcgtcggc tcggcgccag tctcgatctc gctgaagctc
     2821 ggcgtgaccg acaccgccag ctcgagcttg ccgctgccgt cgaggtttgg gccgtcgagg
     2881 aagctgccga tgtcggaaac gttctcgatc aggtccttca gcgtgatctt cgcctgcggg
     2941 tcgaaggcgg gggcgccgat cggcttgagg cccacgtcga cggaaaccgt cagcggcccg
     3001 tcgcccgcga ggttggagat gccgccgtgg ccgtggatgc cgacgaagcc aaagcgcgcc
     3061 tcggcatcga ggccgtcggt ggtcgagccg tcaccgtcag tgtcctcgat ctccccgttc
     3121 gcgttgcgct gcggcgtgac gatcgccgcg gtggcctgcg cctgcgcgtt ctggatgaac
     3181 aggcgatcca gcgggtcgac gccgccaagc tgtccgccct cgatgcggaa gtcgcgcgga
     3241 tcttcctcgg gctcgggctc caccgtgtcg acgccgaaga tggcgaggtc gaacgcggcg
     3301 gtcgagccga ccgcgttgtt gaccttgaag accgccgtgg tctgccgcag gttgtcggtc
     3361 agcaggatgc gggtgtcgcc gtcgctgaag ctgccggtga ccgcgccgcc ggtctgcgag
     3421 ctgatttcgc tcagcacctc gcccagcgtg tcgacctccg cgccgaaggt gatggcgtgg
     3481 tgcacgccgt cgctggtggt gatgaccagg tcgtgcgagt tgccgatgtt cgaggtgccc
     3541 aggccgagct gctgcgccgg gttgccgccc gccacggtga tgctcaggct ggtcgccgcc
     3601 ggattgagcg tcctgaagat gaggttcttg ccgagcgagg tgacctcgat ctcctcttcg
     3661 aagccggcgt cgtccagcgc gcgctgcacg tcgacgatga cgtcgaggat gttgcggttg
     3721 gtggacgtgt cgtccgcgtc gaccgtcacg atcaccggcg caccgccgtt caccgtgtcg
     3781 agcgccacct gcatcaccgc gtcgccggac aggcggccca tcagccccga gacgtccccc
     3841 tggcccttga tcttgagctg gtcgccgtcg tccgcgtccg gttgcgcagt catgctcgcg
     3901 cggaagccga tctgcgtgat ggcgggatcg ccctgctggg cggtgagcac aagcgagctc
     3961 acgccgccga gcgccttcag atttatcttg ttgatcgggt tgccgtcctc gtcttcggcg
     4021 cgctcggcca gcacctgcgc gtcgagcccg gcgagcgcca gggcgtcgtt gatgtcctcg
     4081 acgaggtcgt ccaccgagct gttgtcctcg gtgtcgtcgt tgtcgatgac gatttcctct
     4141 tcgtcgccgc cgtcgaccga gagcttgaac ttggcgttgc ccgacagtcg tccgtaggtg
     4201 gtcgtcacct cgttgggggc ggcgaccacg cgcgcctggc tgaaggcgat gccgcccttc
     4261 aggctcgaga gcagggtgtc gttggcgagg ggcgttcccc cttcgtcgcc cagatacacg
     4321 ccgatgttca gggtgatgcc gccaccggcg gacagctgga tctggctgtc gctcgagagc
     4381 tccgcgaccg gcgccagcgc ctgcagcagg tcgaactgga tcggcacgtc gagcacgccg
     4441 aagttggtgg cgctgtcctt gtcgccgatc tcgagcgcga aggtgagggt ttgtgccagc
     4501 tcgtcgtagg tgaggtaatc acccgcgccg aagatctccg tcagccgcac caccagctcc
     4561 tgcaccgtat cgaacgtcgg gcgattctgg gcgtccagca gcttgctggt gcccgtcgcg
     4621 tcgtcgtcgt cgtcgtggtc gtcgaacagc agcgtgtcgg agaacaggtc ggcgaacctg
     4681 agcgcctcgt cgagcgccgg gccgatcagc ggaatatcct cggcgtcgaa gctgcggcgg
     4741 aagtcctcca gccagttgga gatggaaccg agcaggctga ccagcgtccc ggcatccatg
     4801 ttggtgaaat cgggcagctg gaacaggttc ggcggcagga tgacctgcag ggtgtcggga
     4861 tcggacatgt ccgaccactc gatggtcagc gtggtgtccg ggctggcgac gatgccgagg
     4921 aaatcgactt
//
LOCUS       DASPGN010000019         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1174767, whole genome shotgun sequence.
ACCESSION   DASPGN010000019 DASPGN010000000
VERSION     DASPGN010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174767"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VD788_00595"
     CDS             <1..378
                     /locus_tag="VD788_00595"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXV74787.1"
                     /translation="EPLARFRGYAVAGVPPEIMGIGPVEAVPKLLARQRLTLDRIDVI
                     ELNEAFAAQSLSVLGRLGLAADDPRLNPNGGAIALGHPLGCTGAKLTATALHQLQRCG
                     GRFALVTMCIGLGMGAAGLFERC"
     gene            378..1427
                     /gene="thiL"
                     /locus_tag="VD788_00600"
     CDS             378..1427
                     /gene="thiL"
                     /locus_tag="VD788_00600"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HXV74788.1"
                     /translation="MSAGPQRVAEIGEFELIERLRRLAGPPTEDVVVGIGDDVAVIAT
                     STGRRTVVTCDAQVEGTHFDLARRDPERLGRKAAAINLSDIAAAGGRPRHALVSLVLA
                     PETPVVLVESLYRGLVAELSRFGACIVGGNLASGERLVVDVTLFGDVEPRRLLRRSGA
                     RVGDRLLVTGTLGAAAAGLRLASEPSLGVSPSARQRADEAFETPRARIGEVQTALDCG
                     GVHAAIDVSDGLAADLAHLCDASDVGVELDAASLPIDPAAREVARAAGLDVLHLALGG
                     GEDYELLLAVGPAAVDRLSSAVAERTGTPITPIGTIVDRTAGRRLVGPGGTSPLGRLG
                     FRHFETNGPSQNSGS"
     gene            complement(1409..>3416)
                     /locus_tag="VD788_00605"
     CDS             complement(1409..>3416)
                     /locus_tag="VD788_00605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HXV74789.1"
                     /translation="LRFSDRILIERGIRGSLAIDGPARVAAANELVFDDPRIAPADWT
                     PRLSVLSLDIETDPTASRLLSVALEGCGISDVLLLTPPGYDCPAGAHPCADERELLAE
                     TAARVRRADPDVITGWNVVDFDLTVLARRAASVGIELRLGRHDGMTRVRAGRAGGPAA
                     ARAWISGRVVLDGIALTRGAFIRLDSYSLDNVARDILGKRKTLGGENRATEILRRFEA
                     DRPALVDYNRHDARLVLEVLDRLGLVELAVERSLLTGLTPDRVAGSIAAFDFLYLSEL
                     HRRRIVAPTVGGATARPIEASGGHVLDPQPGLYRNVIVLDFNSLYPSVIRTFQIDPLG
                     YVARPAAGDDLIVAPNGAAFRREHGVLPALLDQLMPRRERAKARGDRVTSQAIKILMN
                     SFYGVLGTSACRFASAALANAVTGFGRELLLWAKACVERDGQRVIYGDTDSLFVLAGT
                     DDAARARAIADRLAERINGELARFVADNYRVDSRLRIEFERLYLRMLLPRLRGRTSGA
                     RKRYVGLVDGAAPTVVFTGMEVVRSDWTALARSVQRELYERLFGDRPVDAYLRETVAQ
                     LRAGAHDGELVYRKRLRRPLDSYVSSTPPHVAAARQLARAPRHRVSYVMTVSGPEPIE
                     RHRSPIDHEHYVQKQLRPVAEPVLDLLGLDFDRVVGDPRQLPLF"
BASE COUNT          493 a         1202 c         1243 g          478 t
ORIGIN      
        1 gagccgctcg cgcgcttccg cggatacgcg gtcgccggcg tgccgcccga gatcatgggg
       61 atcggtccgg tcgaagccgt gccgaagctg ctggcgcgcc agcggttgac gctcgaccgc
      121 atcgacgtga tcgagctgaa cgaggcgttc gccgcgcaaa gcctgtcggt gctggggcgg
      181 ctcggcctcg ccgccgacga cccgcggctc aatccgaacg gcggggcgat cgcgctcggc
      241 catccgctcg gctgcaccgg ggccaagctg acggcgaccg cgctgcatca actgcagcgc
      301 tgcggtgggc gcttcgcgtt ggtcacgatg tgcatcgggc tcgggatggg agcggccgga
      361 ctgttcgagc gctgctgatg tccgccggac cacaacgcgt agcggagatc ggcgagttcg
      421 agctgatcga gcgcctgcga cgtctggccg gaccgccgac ggaggacgtc gtcgtgggaa
      481 tcggtgacga cgtcgccgta atcgcgacct cgaccggacg ccgaacggtc gtgacctgcg
      541 acgcacaggt cgagggaacg cacttcgatc tggcgcgccg cgaccccgag cgactgggtc
      601 gcaaggccgc cgcaatcaac ctcagcgaca tcgcggcggc cggcggacga ccgcgtcacg
      661 cgctggtctc gctcgtgctc gctccggaga cccccgtcgt gctggtcgag tcgctgtatc
      721 gcgggctcgt cgccgagctg tcacgattcg gcgcttgcat cgtcggcggc aacctggcct
      781 ctggcgagcg gctcgtcgtc gacgtgacgc tgttcggcga cgtcgagccc cgccggctgc
      841 tccggcgcag cggcgcccgc gtcggcgacc ggctgctcgt cacgggaacg ctgggtgcgg
      901 cggccgccgg tttgcgtctg gcctccgagc cgtcgctcgg cgtttcgccg tcggcgcggc
      961 agcgggccga cgaagcgttc gagactccgc gggcgcggat cggcgaggtc cagacggcgt
     1021 tggactgcgg cggcgttcat gccgcgatcg acgtcagcga cggcctggcc gccgacctgg
     1081 cccatctgtg cgacgcgagc gacgtcgggg tcgagctcga cgctgcgtcg ctgccgatcg
     1141 atcccgcggc ccgcgaggtc gcgcgcgccg cggggctcga cgtcctgcac ctggcgctcg
     1201 gcggcggcga ggactacgag ctgttgctcg ccgtcggccc ggccgcggtc gaccggctgt
     1261 cgtccgcggt cgccgagcga acggggacgc cgatcacgcc gatcgggacg atcgtcgacc
     1321 ggaccgctgg ccggcggctc gtcgggccgg gaggcacgag cccgctcggc cgcttgggct
     1381 tccgccactt cgagacgaac ggcccgtctc agaacagcgg tagctgacgc gggtcgccga
     1441 cgacgcgatc gaagtcgaga ccgagcagat cgagtaccgg ttcggccacc gggcggagct
     1501 gcttctgcac gtagtgctcg tgatcgatcg gcgagcgatg gcgctcgatc ggttccggac
     1561 cggagaccgt catcacgtag ctcacgcggt gccgcggggc gcgggccagc tggcgcgccg
     1621 ccgcgacgtg cggtggcgtc gagctgacgt acgagtcgag cggtcggcgc aggcgcttgc
     1681 ggtagaccag ctcgccgtcg tgcgcgccgg cgcgcaactg agccaccgtc tcgcgcaggt
     1741 acgcgtcgac cgggcgatcg ccgaacaacc gctcgtacag ctcgcgttgc accgaacgcg
     1801 ccagagccgt ccagtcgctc cgcacgacct ccatcccggt gaagaccacg gtcggagcgg
     1861 caccgtcgac cagcccgaca taccgcttgc gcgcgccgct ggtcctgccg cgcagacgcg
     1921 ggagcagcat ccgcaggtaa agccgctcga actcgatccg cagtctgctg tcgacgcggt
     1981 agttgtcggc gacgaagcgg gccagctcgc cgttgatccg ctcggcgagc cgatcggcga
     2041 tcgcgcgcgc ccgcgccgcg tcgtccgtgc cggcgagcac gaacaagctg tccgtgtccc
     2101 cgtaaatgac ccgctgtccg tcgcgctcga cgcacgcctt ggcccacagc agcagctcgc
     2161 ggccgaatcc ggtgaccgcg ttggccagcg ccgccgacgc gaatcggcag gccgaggtgc
     2221 ccagcacccc gtagaacgag ttcatcaaga tcttgatcgc ctggctcgtc acgcgatcgc
     2281 ccctcgcctt ggcccgctcg cgacgcggca tcaactggtc gagcaacgcg ggtagcacgc
     2341 cgtgctcgcg acggaaggcc gcgccgttcg gggcgacgat caggtcgtcg cccgccgccg
     2401 gccgagcgac gtagcccagc ggatcgatct gaaacgtccg gatcacgctg gggtacaggc
     2461 tgttgaagtc gagcacgatg acgttgcgat agagccccgg ctgcgggtcg aggacgtggc
     2521 caccgctggc ctcgatcggc cgcgccgtgg cgccgccgac cgtcggcgcg acgattcgcc
     2581 ggcggtgcag ctcgctgagg tagaggaagt cgaacgcggc gatcgacccg gcgacgcggt
     2641 cgggcgtcaa gccggtgagc aggctccgtt cgaccgcgag ctcgacgagc cccagccgat
     2701 cgagaacctc gagcaccaga cgggcgtcgt gacggttgta gtcgacgagc gcgggccgat
     2761 cggcttcgaa gcgccggagg atctcggtcg cgcggttttc cccgccgagt gtcttgcgct
     2821 tgccgagaat gtcccgggcg acgttgtcga gagagtagct gtcgaggcgg atgaacgccc
     2881 cgcgcgtcaa ggcgatgccg tcgagcacga cgcggccgct gatccatgcc cgcgccgcgg
     2941 ccgggccgcc cgcgcggccg gcccgcacac gcgtcatccc gtcgtgacgc ccgagtcgaa
     3001 gctcgatgcc gacagacgcg gcacggcgcg ccagtaccgt caggtcgaaa tcgacgacgt
     3061 tccagccggt gatcacgtcg ggatcggccc gccgcacgcg ggccgccgtc tcggccaaca
     3121 gttcccgttc gtcggcgcag ggatgagctc ccgccggaca gtcgtagccg ggcggagtca
     3181 gcaacagcac gtcggagatg ccgcagcctt cgagcgcaac ggacaacagc cgcgacgcgg
     3241 tcgggtcggt ctcgatgtcg agcgagagca cggacaaccg cggcgtccag tcggccggag
     3301 cgatccgcgg gtcgtcgaag acgagttcgt tcgccgcggc cacgcgcgcc ggcccgtcga
     3361 tcgcgagcga gccgcggatg ccgcgttcga tcaggatgcg atcgctgaac cgcagg
//
LOCUS       DASPGN010000020         8527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_589661, whole genome shotgun sequence.
ACCESSION   DASPGN010000020 DASPGN010000000
VERSION     DASPGN010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589661"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..1229
                     /locus_tag="VD788_00610"
     CDS             276..1229
                     /locus_tag="VD788_00610"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV74790.1"
                     /translation="MAVPPHRVVRTLAFAVVVATTVSPASAQAPGENAFDTEYLAPVP
                     DTDDRGGRVNFRFELVSEVVLPGPLSAAGPRPLPGGVEIGVDGGKAIAAWMPESDLEL
                     TPRAPAAPEIEQPPFGLSADGRFRGLPIEEGWILAQRRCPRCKSGWRTRWKIRAPGSQ
                     FARPLVTEHRIYYGTTDNRVYCVKRKSGHRVWVSDVRGRVLRPLELLEVSAPPHPRYF
                     DRNRPLTMGVILVIPDGGAELIALEAKSGERLASYELPENELLVGGAVAHDGQVVVAA
                     QRYSAEDAALLVLGLVPVEGSDAPTPEVAVNDSVPGGSVRD"
     gene            complement(1246..1842)
                     /locus_tag="VD788_00615"
     CDS             complement(1246..1842)
                     /locus_tag="VD788_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXV74791.1"
                     /translation="MTDAVLLGMTDRSPDGTIRVLSPAIGWWCDPPHRGAWVGPGSSI
                     GTIRRLNRRYRLVLPEGAAGRVAGAASTERVRAVEFGQLLFELAPVGESTGAVPSEDE
                     ALLGHPPGAGLEPGMLAIVAPSDGVFYRRASPEAPPFVEVGARVRLGQPVGLVEVMKT
                     FNQIVYDGPAFPMEAEVLELRARDAEEVRAGQILLVVR"
     gene            complement(1839..3563)
                     /locus_tag="VD788_00620"
     CDS             complement(1839..3563)
                     /locus_tag="VD788_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXV74792.1"
                     /translation="MAKVILEPIGKPLHRCYPDEVRRRQQRSMERLNAALDEHRARVR
                     AGWGDEYAQRVEAKGKLTTWDRIARLKDPDSPVLSLGTLVNHGRTFGADERTSPGAGV
                     ATAFVRVHARWLVVIANDNTVASGSWWPQTPEKIQRAQEIALRLRLPVVYLVDCSGLF
                     LPEQANTFPGRTGAGAIFRMNSRLSAAGVPQIAGVHGDCIAGGGYMPIISDVVYMTEQ
                     AYMVIAGAALIKGAKAASIRSVDIGGPDVHVHQSRCADHRVPDDAVLIDCIRNEIGKL
                     PSSACEYYRYGAEPAPPGFAPIELDGIFPDDHRMSYDVRQVLARLVDHSLFWEVLPQV
                     GREMVCGVGRVDGLYAGFIANNPELTAHPVERDLQRPGGILYREGIAKISQFSRTCND
                     DGLPIVWLQDISGFDIGVEAERLGLLGYGSNLIYTNSTNDTPMFTVLLRKASGAGYYA
                     MAGLPYRPLVQLATPITRLSVMEGRTLAIGTFRTKLDERFEVVADSEEEAQKIRRGMR
                     EVEQRIERDMDPVRAASRRDVDEVIKPSELRTYLEALIAMSYQACGYRRVKNPRIWSM
                     HDLDVLTS"
     gene            complement(3570..4928)
                     /locus_tag="VD788_00625"
     CDS             complement(3570..4928)
                     /locus_tag="VD788_00625"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXV74793.1"
                     /translation="MFRSVLIANRGEIASRIVRACRELGIRPVVAYSEADRDAPWLSE
                     ADAAVCIGAARSDASYLDATAVLQAAEQTACQAIHPGYGFLAENAVFAARCEQQGIAF
                     VGPRSAAIRRMGSKALAKRTMAAHGVATIPGSATTLSAADAAAVAEQVGYPVLLKAAA
                     GGGGKGMRRCETAAELPRAFEQAALEADKAFGDPSLYLEKQVRGARHVEFQILCDAYG
                     RGIHLGERDCSIQRKHQKLVEEAPSPAVGESERSEIGMRVARVLAAIGYRGAGTVEFL
                     RDPAGRFYFMEMNTRLQVEHPVTEMITGVDLVVEQLCIAANQPMRRTQDEVKLEGHAI
                     EFRINAEDPQADFAPDPGRVDTLSVPSIDRDGVQVRWDSAIRPGYRIPPYYDSMVGKL
                     IVHGPDRPTALAGAEQALAALQIGGVKTTIGFHRRLLTDRRFRAGHYDIDFLARRGAG
                     KR"
     gene            5040..6248
                     /locus_tag="VD788_00630"
     CDS             5040..6248
                     /locus_tag="VD788_00630"
                     /inference="COORDINATES: protein motif:HMM:NF005744.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXV74794.1"
                     /translation="MKPRTALSDRAGRMPASPIRRLAPLADAASRAGRRVLRLNIGQP
                     DIPTPVEMMSVLREYDEPNLPYGPSQGLPELIEALRLYHRGIGLELEAEHLFVTTGGS
                     EALLFVFAAIADPGDELLVFEPFYTNYGGFAELVGVKTVPVTTRAVDGYHLPPRQQIE
                     AALTDRTRAIVICNPNNPTGTVYTDDELERIAAICRDRGLYLVADEVYREFTYDGRVH
                     RSALTLSGLDQQVVLTDSLSKRVSMCGARVGWIATYNRRMLDAVLRMGQARLCPPTLE
                     QLVGARLTRVPSSYTERVVAEYQRRRDVVVDALQSMDGVTVRRPEGAFYVCPKLPIDD
                     AQSFCEFLLRDFSLDGDTVILAPADGFYATPGLGRDEARIAYVLDTALLDRAMRVLAA
                     ALEAYPARVC"
     gene            complement(6245..7216)
                     /locus_tag="VD788_00635"
     CDS             complement(6245..7216)
                     /locus_tag="VD788_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74795.1"
                     /translation="MGKRIEPADLSRVTTYPLAERANKVDLSRFGRPLDERASLFDFL
                     DSLPDLLAVRSLRGLARAIVDARQADRAVVWAMGAHPIKVGLNPILIRMMDRGFVTAL
                     ALNGAGVIHDWETAAIGGTSEDVAEGLDDGSFGMVEETGRAINAAAREAAEQGIGLGE
                     AVGRGIAQSALPHRDSSVLAAAYRLGLPATVHVAVGGDIVHMHPSADGAAIGAASFTD
                     FKLLAGVLTEIESGVFVNCGSAVQLPEVFLKALTVARNLGHRATPLTTANLDMQRHYR
                     TEQNVLRRPTRGGGRSFSLIGHHEINVPLLAAAIDREFSRRAKPARR"
     gene            complement(7443..8231)
                     /locus_tag="VD788_00640"
     CDS             complement(7443..8231)
                     /locus_tag="VD788_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXV74796.1"
                     /translation="MADPRVLYSTDGGVATLELNDPPANTYSYEMMRELDDAILRARF
                     DSAVHVIVLRGHGDRFFCAGADVGMLRAADPHYKYHFCLHANETLCRLEATPKLVIAA
                     LNGHTVGGGLEVALAADLRVARHGGGKIGLPEVALGVLPGTGGTQRLSRIVGKARAIE
                     MMTTGQLIDFERASQLGLVQHVFPAERFEAELAEFARQFTPPSRAARAVGLIKRAVQS
                     GAEASLSEGLALERELQQQLFQSADAAEGLAAYVEKRKPRFDGR"
     gene            complement(8278..>8527)
                     /locus_tag="VD788_00645"
     CDS             complement(8278..>8527)
                     /locus_tag="VD788_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HXV74797.1"
                     /translation="TSMFEPVHGSAPKYAGTDRANPFAAILTVAMMLDHTGLATAAAR
                     IEGAVAACLGAGECTIDLGGRLGTAATGDAVARRVTDG"
BASE COUNT         1288 a         3055 c         2879 g         1305 t
ORIGIN      
        1 cgcgcgaaca gctccgtcgc ctcgaagatc ggcgtacgga tctcggcgaa gccgtagcgg
       61 gcgaagatcg cgcgcgccga gctctcgagg aactgccagc gagcgatctc gtcgggcagg
      121 atgtcacgcg tgccgcgcag ggctcggtag tgcttgttca ccgcttcgtc gagtggttgg
      181 acaagcgccg cttatatcac atcggagcaa ggcggccaag cgatgggaca gcgacgatca
      241 gcgggcgccg gcgagttccg cgctacaatc gtcgcgtggc cgttccgccc caccgtgtcg
      301 ttcggacgct ggcgttcgcc gtcgtcgtgg cgacgaccgt ctcgccggcg agtgcccaag
      361 caccgggtga gaacgcgttc gataccgagt acctggcccc cgtccccgac accgacgatc
      421 gcggcggccg agtcaatttc cgcttcgaac tggtcagcga ggtcgtgctg ccggggccgc
      481 tgtccgcggc cggtccccgc ccgctgcccg gcggcgtcga gatcggcgtc gacggcggaa
      541 aagcgatcgc ggcgtggatg cccgagtccg atctcgagct gaccccacgc gccccggcgg
      601 cgccggagat cgagcaaccg ccgttcgggc tgtctgcgga tggccggttt cgaggtctcc
      661 cgatcgagga gggctggatc ctggcacagc ggcgctgccc acgttgcaag tcgggttggc
      721 gcacgcgctg gaaaatccga gcgccgggca gtcagttcgc ccggccgctg gtcaccgagc
      781 accggatcta ctacggcacg accgacaatc gcgtctactg cgtcaagcgc aagtcgggtc
      841 accgagtctg ggtcagcgac gtccgcggcc gggtgctgcg gccgctcgag ctgctcgagg
      901 tctccgctcc accccacccg cggtacttcg accggaatcg accgttgacg atgggcgtga
      961 tcctcgtgat cccggacggc ggcgccgagc tgatcgcgct cgaggccaag tccggtgagc
     1021 ggctggccag ctacgagctg cccgagaacg aactgctggt cggcggcgcg gtcgcccacg
     1081 atggccaggt cgtggtcgcc gcccagcgct acagcgcgga ggacgccgcg ctgctcgtgc
     1141 tggggctggt gccggtcgag ggcagcgacg cgccgacgcc ggaggtcgcg gtgaacgaca
     1201 gcgtgccggg tggttcggtg cgggattgac ggcggcgctg ctcggtcagc ggacgacgag
     1261 caggatctgc ccggcgcgca cttcctcggc gtcccgcgcg cgtagctcga gcacctccgc
     1321 ctccattggg aacgccggtc cgtcgtagac gatctggttg aaggtcttca tcacctcgac
     1381 gagcccgacc ggctgaccca atcggacgcg ggcgccgacc tcgacgaatg gcggcgcctc
     1441 cggcgacgcg cgacggtaga acacgccgtc gctcggtgcg acgatcgcca gcatccccgg
     1501 ttcgaggccg gcgcccggcg ggtgcccgag cagcgcctcg tcctcgctcg gcacggcccc
     1561 cgtgctctca ccgaccggcg cgagctcgaa cagcagctgg ccgaactcga ccgcgcgcac
     1621 ccgctcggtc gaggcggcac cggccacgcg gccggccgcc ccctcgggca acaccaagcg
     1681 gtagcgccgg ttgagccggc ggatggtgcc gatcgagctg cccggtccga cccacgcgcc
     1741 gcggtgcggt ggatcgcacc accagccgat agcgggcgac agcacgcgga tcgtcccgtc
     1801 gggcgatcgg tcggtcatgc cgagcagaac cgcgtccgtc atgaggtcag cacgtcgagg
     1861 tcgtgcatgg accagatccg cgggttcttc acgcgtcggt agccacaggc ctggtagctc
     1921 atcgcgatca gcgcttcgag ataggtccgc agctcggacg gcttgatcac ctcgtcgacg
     1981 tcgcggcgcg acgccgctcg caccggatcc atgtcccgct cgatccgctg ctcgacctcc
     2041 cgcattccac gccggatctt ctgcgcctcc tcctcgctgt cggccaccac ctcgaaccgc
     2101 tcgtcgagct tggtgcggaa cgtgccgatt gccagcgtac ggccctccat caccgacaac
     2161 cgggtaatcg gcgtcgccaa ctggaccaac ggccggtagg gtagcccggc catcgcatag
     2221 tagccggctc cggaggcttt gcgcagcagc acggtgaaca tcggcgtgtc gttcgtcgag
     2281 ttggtgtaga tcaggttcga gccgtagccg agcaagccca agcgttcggc ctcgacgccg
     2341 atgtcgaatc cggagatgtc ctgcaaccag acgatcggca ggccgtcgtc gttgcaggtc
     2401 cgcgagaact gcgagatctt ggcgatcccc tcgcgataga ggatacctcc cgggcgctgc
     2461 aggtcgcgct cgaccgggtg cgcggtcagc tcggggttgt tggcgatgaa tcccgcgtac
     2521 aggccgtcga cccgcccgac gccgcacacc atctcgcgtc cgacctgcgg caggacctcc
     2581 cagaacaacg aatggtcgac caagcgggcc agcacctgac ggacgtcgta gctcatccgg
     2641 tggtcgtcgg ggaaaatacc gtcgagttcg atcggggcga agccgggcgg tgccggctcg
     2701 gcgccgtagc gatagtactc gcatgcgctc gacggcagct tgccgatctc gttgcggatg
     2761 caatcgatca gcacggcgtc gtccgggacg cggtgatcgg cgcagcggga ctggtggacg
     2821 tggacgtcgg gtccgccgat gtcgaccgag cggatgctcg ccgccttggc gcccttgatc
     2881 aatgccgcgc cggcgatcac catgtaggcc tgctccgtca tgtagaccac gtcgctgatg
     2941 atcggcatgt agccgccgcc ggcgatgcag tcgccgtgga cccccgcgat ctgcggtaca
     3001 ccggcagccg acagccgtga gttcatccgg aagatcgccc ccgcgccggt ccggccgggg
     3061 aaggtgttgg cctgctccgg caggaacaac ccggagcagt cgacgagata gacgaccggc
     3121 agccgcagtc gcagtgcgat ctcctgggcc cgctggatct tttcgggtgt ctgcggccac
     3181 cacgaaccgg acgcgaccgt gttgtcgttg gcgatcacga ccagccaacg cgcgtgcaca
     3241 cgcacgaagg cggtggcgac gccggccccc ggtgaggtgc gctcgtcggc gccgaacgtg
     3301 cggccgtgat tgaccagcgt gccgagcgag agcaccggac tgtccgggtc tttcagcctg
     3361 gcgatgcggt cccaggtcgt cagcttgccc ttggcctcga cccgttgcgc gtattcatcg
     3421 ccccagcccg cacggacgcg cgcccggtgc tcgtcgagcg ccgcgttcag ccgttccatg
     3481 gagcgctgct ggcgccgccg cacctcgtcg gggtagcacc ggtggagcgg cttaccgatc
     3541 ggctcgagga tcaccttggc catctcgacc tagcgctttc cggccccgcg acgtgcgagg
     3601 aagtcgatgt cgtaatgccc ggcacggaag cgacggtccg tgagcaaacg acggtggaaa
     3661 ccgatcgtcg tcttgacacc gccgatctgt agcgccgcca gcgcctgctc ggcaccggcc
     3721 agcgccgtcg gtcggtcggg cccgtggacg atcagcttgc cgaccatcga gtcgtagtac
     3781 ggcggaatcc ggtagcccgg tcggatcgcc gaatcccacc gcacctgcac gccgtcgcgg
     3841 tcgatcgacg ggacgctcag cgtgtcgaca cgacccggat cgggcgcgaa atccgcctgc
     3901 ggatcctcgg cattgatgcg gaactcgatc gcgtggcctt cgagcttgac ctcgtcttgc
     3961 gtgcggcgca tcggttggtt ggcggcgatg cacaactgct cgacgacgag gtcgacgccg
     4021 gtgatcatct ccgtgaccgg atgctcgacc tgcaatcggg tgttcatctc catgaagtaa
     4081 aagcgaccgg cgggatcgcg caggaactcg accgttcccg caccacgata gccgatcgcc
     4141 gccagcacgc gcgccacccg catgccgatc tcggaccgtt cgctctcgcc gacggccggc
     4201 gacggggctt cctcgacgag cttctggtgc ttgcgctgga tcgaacagtc gcgctcgccg
     4261 aggtggatgc cccggccgta ggcgtcgcac aggatctgga actcgacgtg ccgcgcgccg
     4321 cgcacctgct tctccagata gagcgacggg tcaccgaacg ccttgtccgc ctcgagcgcg
     4381 gcctgctcga acgcccgcgg cagctcggcg gcggtctcgc atcggcgcat ccctttgccg
     4441 ccgccgcccg ccgcggcctt gagcaacacc gggtagccga cctgctccgc caccgccgcg
     4501 gcgtcggcgg cggacaacgt cgtcgccgac ccgggtatcg tcgcgacgcc gtgcgcggcc
     4561 atcgtccgct tggcgagtgc cttgctgccc atgcggcgaa tcgcggcgga gcggggaccg
     4621 acgaaggcga tgccctgctg ctcgcaacgc gcggcgaaga ccgcgttctc tgccaagaag
     4681 ccgtagcccg gatggatcgc ctggcacgcc gtctgttcgg cggcctgcag caccgcggtc
     4741 gcgtccaaat aggatgcgtc cgaccgcgcc gcgccgatgc aaacggcggc gtcggcctcg
     4801 ctcaaccacg gagcgtcgcg atcggcttcg gaatacgcca cgaccggacg gatgcccagc
     4861 tcgcggcatg cgcggacgat gcgcgacgcg atctcccccc ggttggcgat caacacgctg
     4921 cggaacacgc ggcccccttc gtcggcctga gtctagcctc cgcggcggac tcgcggcaaa
     4981 cctaccgccg ggcgatcccg ttcgctacac tggaactgct cgtcgaggga ggagggtcga
     5041 tgaagccgcg aaccgctctg tccgaccggg ccggccgaat gccggcctcg ccgatccggc
     5101 ggctcgcgcc gttggccgat gccgccagtc gcgccggacg acgcgtcctg cgactgaaca
     5161 tcggccaacc ggatatcccc acaccggtcg agatgatgtc cgtgctgcgc gagtacgacg
     5221 agccgaacct gccctacggc ccgtcgcagg gtctgcccga actgatcgaa gcgctgcgtc
     5281 tctaccaccg gggcatcgga ctcgagctcg aggcggaaca cctgttcgtc accaccggcg
     5341 gtagcgaggc gctgctgttc gtcttcgccg cgatcgccga tcccggcgac gagctgctgg
     5401 tgttcgagcc gttctacacg aactacggcg gattcgccga gctcgtcggc gtcaagaccg
     5461 tcccggtgac cacacgcgcc gtcgacggct accatctccc cccgcgccag cagatcgagg
     5521 cggcgttgac cgaccggacc cgcgcgatcg tcatctgcaa cccgaacaat ccgacgggaa
     5581 cggtctacac cgacgacgag ttggagcgga tcgcggcgat ctgccgcgat cgcgggctgt
     5641 atctcgtcgc ggacgaggtc taccgcgagt tcacctacga cggccgggtg caccgctcgg
     5701 cgctgacgct gtccggtctc gaccagcagg tcgtgctgac cgacagcctg tcgaagcgcg
     5761 tgtcgatgtg tggagcccgc gtcggctgga tcgcgaccta caaccggcgg atgctcgacg
     5821 cggtgctgcg catgggccag gcccgcctct gcccgccgac gctcgagcaa ctggtcggcg
     5881 cccgactgac ccgggttcct tcgagctaca ccgagcgggt cgtcgccgag taccagcgca
     5941 ggcgcgacgt cgtcgtcgat gccttgcagt cgatggacgg tgtgacggtg cggcgcccgg
     6001 agggcgcgtt ctacgtctgc cccaagctgc cgatcgacga cgcgcagagc ttctgcgagt
     6061 tcctgctgcg ggacttctcg ttggacggcg ataccgtgat actggcgccg gcggacgggt
     6121 tctacgcgac cccgggtctc gggcgcgacg aggcccgcat cgcatacgtg ctcgacaccg
     6181 ctctgctcga ccgcgcgatg cgcgtactgg ccgccgctct cgaggcctac cccgcgcgcg
     6241 tctgctagcg gcgtgccggc ttcgcccgtc gggagaactc gcgatcgatc gcggcggcca
     6301 gcagcggcac gttgatctcg tgatgcccga tcaggctgaa cgaccgcccg ccgccgcggg
     6361 tcggacgccg gagcacgttc tgctcggtgc ggtagtgccg ctgcatgtcg agattggccg
     6421 tcgtcagcgg tgtcgcgcgg tggccgagat ttcgcgccac ggtcagcgcc ttgagaaaga
     6481 cctcgggtag ctggacggcg gagccgcagt tgacgaagac gcccgattcg atctcggtca
     6541 gcacgcccgc cagcaacttg aaatcggtga agctcgccgc gccgatcgcc gccccgtcgg
     6601 ccgaggggtg catgtggacg atgtcgccgc cgaccgcgac gtgaacggtc gccggtagtc
     6661 ccagtcggta ggctgcggcc agcacgctcg agtcccggtg cgggagcgcc gattgcgcga
     6721 tgccgcgccc gaccgcctcc cccagaccga tgccctgctc cgccgcctca cgggccgcgg
     6781 cgttgatcgc gcgtcccgtc tcctcgacca tgccgaagct gccgtcgtcc aacccctcgg
     6841 cgacgtcctc cgacgtgccg ccgatcgccg ccgtctccca gtcgtggatc accccggccc
     6901 cgttcagcgc cagcgccgtc acaaagccgc gatccatcat gcggatcagg atcggattca
     6961 aaccgacctt gatcgggtgc gcgcccatcg cccagaccac cgcccggtcg gcctgccgcg
     7021 cgtcgacgat cgcgcgcgcc aaaccgcgca gcgaccggac ggcgagcaga tcgggcagcg
     7081 aatcgaggaa gtcgaacaac gacgcccgct cgtccagcgg ccggccgaac cgggacagat
     7141 cgaccttgtt cgctcgctcg gcgagcgggt aggtcgtgac gcgagacaaa tcggccggct
     7201 cgatccgctt ccccatctcg accccctact tcagacgcta cccgcctggc cctcgcgtcg
     7261 cgtgatgacg gcgacgccgc tggaatcgct ccagacgtcg atcgacgtgc ggcacatgtc
     7321 gagcgatcga tcgaccgcca acacgcaacc ggccgcctcg acgagggaac cgcccgaaag
     7381 ctcgtcgcgc ggtttgtccg acgacgtccg gggcctgcag cagactgcgc acggccgggg
     7441 cgctaccgtc cgtcgaaccg cggtttgcgc ttctcgacgt aggccgccag tccctcggcg
     7501 gcatcggcgc tctggaacag ctgctgctgc agctcccgct cgagcgccag accctcgctc
     7561 agcgaggcct cggcacccga ttgcactgcg cgcttgatca gcccgacggc ccttgccgcc
     7621 cgcgagggcg gcgtgaactg ccgcgcgaac tccgcgagct cggcctcgaa gcgctcggcc
     7681 ggaaagacgt gttggaccag gcccagctgg ctggcccgct cgaagtcgat caactgaccg
     7741 gtcgtcatca tctcgatcgc gcgggccttg ccgacgatcc gactcaggcg ctgggtcccg
     7801 ccggtcccgg gcagtacccc gagcgcgacc tcgggcagac cgatcttccc accgccgtga
     7861 cgtgcgacgc gcaggtccgc ggccagcgcg acctccagcc cgccaccgac ggtgtggccg
     7921 ttgagcgccg cgatcaccag cttgggcgtc gcctcgaggc ggcagagcgt ctcgttggcg
     7981 tgcaggcaga agtgatactt gtagtgtggg tccgcggcgc ggagcatccc gacatcggcc
     8041 ccggcgcaga agaagcgatc gccgtgaccg cggagcacga tgacgtggac cgcgctgtcg
     8101 aagcgggccc gcaggatcgc gtcgtccaat tcgcgcatca tctcgtagct gtaggtgttg
     8161 gccggcgggt cgttcagctc gagcgtcgcg acgccgccgt cggtcgagta cagcacgcgt
     8221 ggatcggcca tctcaccccc caagcgccgg ccgccgactc gatcgcggcg tggcgcgtca
     8281 accgtcggtc acacgccgcg caaccgcgtc cccggtggcc gcggtcccca gccggccgcc
     8341 gaggtcgatc gtgcactccc cggcgcccaa gcaggccgcc accgcaccct cgatccgcgc
     8401 cgcggccgtc gccagcccgg tgtgatccag catcatcgcg acggtcagaa tcgccgcgaa
     8461 cgggttggcg cggtccgtgc cggcgtattt gggcgccgag ccgtgcaccg gctcgaacat
     8521 cgaggtc
//
LOCUS       DASPGN010000021         7355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_589933, whole genome shotgun sequence.
ACCESSION   DASPGN010000021 DASPGN010000000
VERSION     DASPGN010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7355
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589933"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VD788_00650"
     CDS             complement(<1..783)
                     /locus_tag="VD788_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HXV74798.1"
                     /translation="MFPNPHETATEEKRSALAITAYSACNGLGTTTDEVLAALAASRS
                     GLRPPPLPVDFATLCGVVDESLPAVSGRFSAYDSRQARIALTTLDPIRATLDAAIRRW
                     GRERIGLVLATSTGGIAVSEEAHRSLRRTGRVPPDYDFGRRHAFFAWAELIRLVTGVA
                     GPGHVVSTACSSGGKALASARRLIFAEIVDAVLVGGVDSLALTTICGFGGLGVLSAEP
                     CRPFSARRNGMNIGEGGAMLLLERAGQARATLLGTGESSDARN"
     gene            899..2224
                     /locus_tag="VD788_00655"
     CDS             899..2224
                     /locus_tag="VD788_00655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXV74799.1"
                     /translation="MAFREDPAAGAATDYDVAVIGGGPAGATAALCAARKGMRVALLE
                     QTKHPRFHIGESFLPRNMTLLRELGLEERVKRLPQTLKLGASFALGHETTPTDFFFEG
                     GLLEATPDTFNIERAPFDRLLLDCAREAGAQVVEDCTVREISRLSEHGVVLETHHGDG
                     GRATVRARHLIDASGQSTVVGRQLKTRRTLPDRRRVAYYGHFRDVERFTDVKAGFPLV
                     VMCREGWFWFIPIDEERMSIGLVVDADLAARAGVPAREMLAWGIARCPLARVRVRHAE
                     WPPRNHVTADFSYTCKPYAGEGYFLVGDAATFVDPIFSTGVCLGMMSARTAVETIERI
                     RDGRLSPHRGRSTYCRYVEQSSSVFFRLVRSYYRHSFRELFLHGYGPLRMHCAVISIL
                     CGHVFPRPRLALRWRLRLFEYLVKLQRLVPIVPRREQFSLLTGTLVSGR"
     gene            complement(2228..3385)
                     /locus_tag="VD788_00660"
     CDS             complement(2228..3385)
                     /locus_tag="VD788_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HXV74800.1"
                     /translation="MDRGASIPRRRWRVGRALSELHYRLRTEGDSDLRTAGSVGLGTV
                     VGCLPVYGAHLLLCVALARLLRVSRIKTYLAAHVNNPLSAPWLLYLEYGIGHQVFGGE
                     WPRVSVAGLRSVGIAELGRDLIVGSLVVGVALGAVMAGLAYAVRVRARAEGLFDRLRE
                     ETSRRYLDAGMFHWEFVRGKLRYDPVYRTILETGVLPADGRLVDLGCGRGIVLALLGT
                     ARALHRTGGWTTELPAPPSRLRLAGVERRESTARVARLATGGEVPIEVEDLCDHEPAA
                     ADAVLLLDVLHYLSAEHQARLIRRIAAKLPRGGIVLIREADAARGLRFRFSAWSERLA
                     AILRLRWRQRFCYRAARSWCELIESAGFETRVWPMWGRTPHGNVLIVAHKT"
     gene            complement(3396..4229)
                     /locus_tag="VD788_00665"
     CDS             complement(3396..4229)
                     /locus_tag="VD788_00665"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXV74801.1"
                     /translation="MRGRSTAVGESKGSVVLRSRFVRFSLGLHVLAALVLLVSLRPWR
                     EVVAVVLIDHLVLVGASLWPRSSLVGPNLCRLPSDAEANAVGLTFDDGPDPEVTPAVL
                     EMLDRHRVAATFFCIGRRAARHPELVAEIARRGHRVENHSYRHVQWFCFLGPAALRRE
                     IDRAQDALGRQAGAAPRYFRAPAGLRSPWLDPVLVSRGLELVSWSRRGFDTVTRDADR
                     VVRRLLRRVAPGDILVLHDGSSARDRHGRPVVLSTLRALLVELEHRGLRAVAVPPAAR
                     R"
     gene            complement(4233..5678)
                     /locus_tag="VD788_00670"
     CDS             complement(4233..5678)
                     /locus_tag="VD788_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXV74802.1"
                     /translation="MRRDPLVEAFDRLVKRRTGPPIVVSQRGSVTPVEIDRAARSIGR
                     LVRDTGIDPGAPLGVVAGNGAGFLAILLAGLRLGHAPILIDRQVTDLELHRVLERLDA
                     GGHVRCVAAFSRGTQAFRIERRSRRTRDRLHTRSPAVIKLTSGSTGSPRGILTSTEAL
                     LSDDEALDRSMEIRSSDRLLAAVPFSHSYGLSSLVLPALVRGAPLVVPEPTGIFAPLE
                     AARDFGATIFPTVPAYLAALLRVSGAPPLVDSVRRVISAGARLMPEVAAGFRERFGRG
                     VHAFYGASECGGIAYDPTGTAAERETVGTPVSGVRVDLEAVDGLPADRCRRIVVRSPA
                     VADGYLPDRDARLGGGLFVSDDLGVRRDDELALVGRLNRLINVRGNKVDPTEVERVIS
                     ELSVVDEVRVVGVVNEKTGDHRVSVVVACRPGAVSPDEIRAWCRGRLSGFKLPRSIHV
                     LERIPRDERGKVDRASLARLARASSPRPKRG"
     gene            5808..6095
                     /locus_tag="VD788_00675"
     CDS             5808..6095
                     /locus_tag="VD788_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HXV74803.1"
                     /translation="MPADSSPTVDDLKRLIISSLGLEGLTPEAIDDDLPLFGEGLGLD
                     SVDALEIIVALEKRLGIKVGSHQIEPADFATVGSIHRFIEKQRARAAGPPG"
     gene            6092..7315
                     /locus_tag="VD788_00680"
     CDS             6092..7315
                     /locus_tag="VD788_00680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXV74804.1"
                     /translation="MSRDARLRVVVTGVGVVSAWGWGADRFWRGVLSGKHALGRLTRF
                     EPDEYATRIAGEVPLPAEAVPARPRLSFADRFALAAAREAVGHAGLDDGRSRVGTGVF
                     FGSSTGGMYEGEAYYRSLKDRRGGRVRIAALASQQISAPAEAVARELGVCGPVETMSS
                     ACASSTLSIGAAVDAVRDGSVRVALAGGADSLCRTTYGGFNALRAVDERPCRPFRADR
                     AGLSMGEGAAVLVLEREADAIERGAVPLAEAIGSGASADANHMTAPDSTGRVPAQAMD
                     RALADAGVHPDEVDFINAHGTGTELNDLAEYRALEQVFGTRATEIPLTSTKGSVGHLL
                     GSAGAIEAVATVLCLHHRQVHPTPGGGAIDPRTPVRLVTDSALSVHPLEVGLSLNLAF
                     GGCNAALVFSRWQGE"
BASE COUNT         1127 a         2631 c         2578 g         1019 t
ORIGIN      
        1 gttgcgggcg tccgagcttt cgccggtccc gagcagcgtg gcgcgcgcct ggcccgctcg
       61 ctcgagcagc agcatcgcac cgccctcgcc gatgttcatc ccgttccgcc gcgcggagaa
      121 cggccggcac ggctccgccg acagtacgcc caacccgccg aacccgcaga tcgtggtcag
      181 cgccaggctg tcgaccccgc cgacgaggac ggcatcgacg atctcggcga agatcaagcg
      241 acgcgccgag gccagcgcct tgccgccgga cgagcacgcg gtcgagacga cgtggcccgg
      301 accggcaaca ccggtcacga gccggatcag ctcggcccag gcaaagaagg cgtgccgtcg
      361 cccgaagtcg tagtccggcg gcacgcgacc cgtgcgccgc aacgagcggt gggcctcctc
      421 ggagacggcg atccctccgg tgctcgtcgc cagcaccagc ccgattcgct cccgccccca
      481 gcgccggatc gcggcgtcga gcgtggctcg aatcggatcg agcgtcgtca aggcgattcg
      541 tgcctggcgg ctgtcgtagg cggagaatcg gccggagacc gccggcaacg attcgtcgac
      601 gacgccgcac aaggtggcga agtcgaccgg caacggcggc ggccgcagcc cgctccggga
      661 ggccgcgagc gcggcgagca cctcgtcggt cgtcgtgccg agcccgttgc atgccgagta
      721 ggcggtgatc gcgagcgcgg agcgtttttc ctcggtggcc gtttcgtggg gattcgggaa
      781 cacgcctcga gctactccac atccgtcccg gctcgacgga gtgtagcgcg attgtcaccc
      841 cggcgcggcg ccccgatcgt gtaagatcgg ccggagttcc gaatctttgg gaagtcggat
      901 ggccttccgc gaagatcccg cggccggcgc ggcgaccgac tacgacgtcg cggtgatcgg
      961 cggcggaccg gcgggcgcca ccgccgcgct ctgtgccgcc cgcaagggga tgcgcgtcgc
     1021 gctcctcgag cagacgaagc accctcggtt ccacatcggt gagtcgttcc tgccgcggaa
     1081 catgacgctg ctgcgcgagc tgggactgga agaacgggtc aagcggctgc cccagaccct
     1141 caagctcggc gcctcgttcg cgctgggtca cgaaacgacg ccgaccgact tcttcttcga
     1201 gggcgggctg ctcgaggcga ctcccgacac cttcaacatc gagcgcgctc cgttcgaccg
     1261 gctgttgctc gattgcgcga gggaggccgg ggcacaagtc gtcgaggatt gcaccgtgcg
     1321 cgagatctcc cggctctccg agcacggcgt cgttctcgaa acgcaccacg gcgacggcgg
     1381 tcgcgccacg gtgcgtgccc gccatttgat cgacgcgagc ggccagtcga ccgtcgtcgg
     1441 ccggcagctc aagacgcgac gcaccttgcc cgatcgccgg cgggtcgcct actacgggca
     1501 ttttcgcgac gtcgagcgct tcaccgatgt caaggccggg ttcccgctgg tggtcatgtg
     1561 ccgcgagggg tggttctggt tcatcccgat cgacgaggag cggatgagca tcggtctggt
     1621 ggtcgacgcc gacctcgccg cgcgggccgg cgtcccggcg agggagatgc tggcctgggg
     1681 gatcgcgcgc tgtccgctgg cgcgcgtcag ggtcagacac gcggaatggc cgccgcggaa
     1741 ccacgtgacg gcggatttca gctacacctg caaaccgtac gcgggcgagg gctacttcct
     1801 cgtcggcgac gccgccacct tcgtcgatcc gatcttctcg accggcgtct gcctcggcat
     1861 gatgtcggcg cggacggccg tcgagacgat cgagcggatt cgcgacggcc ggctgtcacc
     1921 gcaccgcggt cgatcgacct actgtcgcta cgtcgagcag agctcgtcgg tcttcttccg
     1981 gctggtgcgc agctactatc gccactcctt tcgcgagctg ttcctgcacg gctacggccc
     2041 gctgcggatg cactgcgcgg tcatctcgat cctgtgcggc cacgtcttcc ccaggccgag
     2101 gctcgcactc cgctggcgac tgcgcctgtt cgagtacctg gtcaagctgc agcgcctcgt
     2161 accgatcgtc ccgcgacgcg agcagttctc gctgttgacg gggacgctcg tgtcgggccg
     2221 ctagccgcta cgtcttgtgc gccacgatca gcacgttgcc gtgcggggtg cgcccccaca
     2281 tcggccaaac gcgggtctcg aagcccgcgc tctcgatcaa ctcgcaccag gatcgcgccg
     2341 cgcgatagca gaaccgctgt cgccaacgca ggcggagaat cgccgccaga cgctcgctcc
     2401 aggcgctgaa gcggaagcgc aggccgcggg cggcatcggc ttctcgaatc aacacgatac
     2461 ccccgcgtgg cagcttcgcg gcgatccgcc ggatcagccg ggcctggtgc tcggcggaca
     2521 ggtagtgcag cacgtcgagc agcagcaccg cgtcggccgc cgccggctcg tgatcgcaca
     2581 gatcctcgac ctcgatcggc acctcgccgc ccgtcgccag cctcgcgacc cgcgccgtcg
     2641 attcccgtcg ctcgacgccg gcgagccgca gccgcgacgg aggggcgggc agctccgtcg
     2701 tccaccctcc ggtccggtgc agcgctcgtg cggttccgag tagggccagc acgatcccgc
     2761 gcccgcagcc gagatcgacc aagcggccgt cggcgggcaa cacacccgtc tcgaggatcg
     2821 tgcggtaaac cgggtcgtac cgcagcttgc cgcgcacgaa ctcccaatgg aacatgcccg
     2881 catcgagata acgccgggag gtctcctcgc gcagcctgtc gaacaggccc tccgccctgg
     2941 cgcgaacgcg gacggcgtac gccagccccg ccatcactgc gcccaacgcc acgccgacca
     3001 ccaggctgcc gacgatcagg tcgcgaccga gctcggcgat gccgaccgag cgcagccccg
     3061 cgacgctgac ccgcggccat tcgccaccga acacctgatg cccgatcccg tactcgagat
     3121 agagcaacca cggcgccgac agcgggttgt tgacgtgcgc cgcgagatag gtcttgatcc
     3181 ggctgacccg caacagccga gccagggcga cgcacagcaa gagatgcgcg ccgtagaccg
     3241 gcaggcagcc gacgaccgtg cccagcccca ccgagccggc cgtgcgcagg tcggaatcgc
     3301 cctcggttcg cagccggtag tgcagctccg acagcgcccg gccgacgcgc catcgccgcc
     3361 gggggatcga cgctccgcgg tccaacgcga tccgctcagc gccgggcggc cgggggtacg
     3421 gccaccgcac gcagcccgcg atgctcgagc tcgacgagca gggcgcggag tgtcgacagc
     3481 acgaccgggc gcccatgccg gtcccgcgcc gaagagccgt cgtgcaggac gaggatgtcg
     3541 ccgggcgcga cccggcgcag caggcgccgc acgacccggt cggcgtcgcg ggtcacggtg
     3601 tcgaacccgc gccgcgacca gctcaccaac tcgagcccgc gcgagacgag caccgggtcg
     3661 agccaggggc tcctcaggcc cgccggcgcg cggaagtagc ggggggcggc gcccgcctgc
     3721 cgcccgagcg cgtcctgggc acgatcgatc tcgcggcgga gggccgcggg tccgaggaag
     3781 cagaaccact ggacgtgacg atacgagtga ttctccacgc ggtggccgcg tcgcgcgatc
     3841 tccgcgacga gctccggatg acgcgccgcg cgccggccga tgcagaagaa ggtcgccgcg
     3901 acgcgatggc ggtccagcat ctcgagcacc gcgggcgtca cctcgggatc gggcccgtcg
     3961 tcgaaggtca gcccgaccgc gttcgcttcc gcgtcagacg gcagcctgca caggttcggc
     4021 ccgacgagtg agctgcgcgg ccagagactc gccccgacca gcacgagatg atcgatcagc
     4081 acgacggcga ccacctcccg ccatggccgc agcgacacga gcaagaccaa cgccgcgagc
     4141 acgtggagcc cgagcgagaa ccgtacgaac cgcgatcgca gcacgacgct gcctttcgat
     4201 tcgccgaccg cggtcgatcg cccccgcacc gtctaacctc gcttcggtcg cgggctcgac
     4261 gcacgggcaa ggcgcgccaa ggacgcgcga tcgaccttgc cgcgctcgtc ccgcggaatc
     4321 cgctcgagca cgtggatgct tcgcggcagc ttgaagccac tgagacgtcc gcgacaccag
     4381 gctcggatct cgtccgggga gaccgcgccc ggtcgacagg cgaccaccac gctcacgcgg
     4441 tggtcaccgg tcttctcgtt gacgacgccg acgaccctga cctcgtcgac gacggacagc
     4501 tcggagatca cgcgctcgac ctcggtcggg tcgaccttgt tgccacggac gttgatcaga
     4561 cggttcaacc ggccgaccag cgccagctcg tcgtcacgtc gcacgccgag gtcgtccgaa
     4621 acgaacaacc caccgccgag acgagcgtcc cggtcgggca ggtagccgtc ggccaccgcg
     4681 ggcgagcgca cgacgatccg ccgacatcga tccgcgggga gtccgtcgac ggcctcgaga
     4741 tcgacgcgga cgcccgacac cggtgtgccg accgtctccc gctctgccgc cgttcccgta
     4801 ggatcgtagg cgattccgcc gcactcgctc gcgccgtaga aggcgtgcac gccgcgaccg
     4861 aaccgctcgc ggaacccggc ggcgacttcc ggcatcagcc gcgccccggc cgagatcacc
     4921 cgacggaccg aatcgaccag cggcggcgcg ccgctcaccc gcagcaacgc ggcgaggtag
     4981 gccggaacgg tcgggaagat cgtggcgccg aaatcgcggg cggcttcgag cggtgcgaag
     5041 atcccggtcg gctcgggcac gacgagcggt gctccgcgga cgagcgccgg gagcacgaga
     5101 ctcgacaggc cgtagctgtg ggagaacggg acggcggcga gcagtcggtc ggaggagcgg
     5161 atctccatcg atcgatcgag cgcctcgtcg tcactgagca gcgcttcggt cgaggtcagg
     5221 atcccgcgcg ggctccccgt cgagcccgac gtgagcttga tcaccgcggg cgaccgagtg
     5281 tgaaggcgat cgcgcgtgcg gcgcgagcga cgctcgatgc ggaacgcctg cgttccccgc
     5341 gagaaggcgg cgacgcaccg aacgtggccg ccagcgtcca gtcgctcgag gacgcgatgc
     5401 agctcgagat cggtcacctg ccgatcgatc agtatcggcg cgtgcccgag acgcagcccc
     5461 gcgaggagga tcgccaggaa gccggctccg ttgccagcga cgacgccgag cggcgccccc
     5521 gggtcgatgc cggtgtcccg cacgagccgt ccgatcgagc gcgcggcccg atcgatctcg
     5581 accggggtga cgcttccgcg ttgggacacg acgatcggcg gcccggttct tcgtttgacc
     5641 agccgatcga acgcttcaac gagtggatcg cgcctcatca agaacgggtc cgagtcgccg
     5701 ggcgactcat tgtagacgac gaagtcgacg tagtatactc ggctgccggt cgccgtcgtc
     5761 gggtcgatgc ctgttctgct ctcgcgatcg cgtccgaggt ggatgtcgtg cccgcagatt
     5821 cctcgccgac ggtcgatgac ctcaagcgac tgatcatctc cagcctcggg ttggagggtc
     5881 tgacgccgga ggcgatcgac gacgacctgc cgctgttcgg cgaaggactg gggttggatt
     5941 cggtcgacgc gctcgagatc atcgtcgcgc tcgagaaacg actcgggatc aaggtcggca
     6001 gccaccagat cgagccggcg gacttcgcca cggtcggaag catccaccgc ttcatcgaaa
     6061 agcagcgagc ccgggcggcg gggccccctg gatgagccgg gacgcacgcc tccgtgtcgt
     6121 ggtgaccgga gtcggcgtcg tatccgcgtg gggatgggga gccgaccggt tttggcgtgg
     6181 cgtgctctcc ggcaagcacg cgctcgggcg gttgacgcgt ttcgagcccg acgaatacgc
     6241 gacccgcatc gcgggcgagg tcccgctccc cgccgaggcc gtgcccgctc gaccccggct
     6301 gagctttgcc gaccgtttcg cactggccgc ggcgcgtgag gcggtcggcc acgcgggcct
     6361 cgacgacggg cggtcccgtg tcggcaccgg tgtcttcttc ggcagcagca cgggcgggat
     6421 gtacgagggc gaggcctact accggtcgtt gaaggaccgg cgcggcgggc gggtacgcat
     6481 cgccgcactc gcctcgcagc agatcagcgc accggccgag gcggtggcgc gcgagcttgg
     6541 cgtgtgtggt ccggtcgaga cgatgtcgtc ggcctgcgcc tcgtcgacgc tttcgatcgg
     6601 agccgccgtc gatgccgtcc gtgacggctc ggtgcgggtc gcgctcgccg gcggagcgga
     6661 cagcctgtgc cgcacgacct acggcgggtt caacgccttg cgcgccgtcg acgagcgacc
     6721 atgccgtccc ttccgcgccg atcgagcggg cctgtcgatg ggcgagggtg cggcggtgct
     6781 ggtgctcgag cgggaggcgg acgcgatcga gcggggagcg gtgccgctcg cggaggcgat
     6841 cgggtcgggg gcgtcggcgg atgcgaacca catgaccgct cccgactcca ccggccgcgt
     6901 ccccgcgcag gcgatggacc gggcgctcgc cgacgccggg gtgcacccgg acgaggtgga
     6961 cttcatcaac gcgcacggga cggggacgga gctgaacgac ctggccgagt accgggcgct
     7021 cgagcaggtg ttcggcacgc gggcgacgga gatcccgctc acctcgacca agggttccgt
     7081 cgggcacctg ctgggcagcg ccggcgcgat cgaggccgtc gcgaccgtgc tgtgcctcca
     7141 tcatcggcag gttcacccga cgccgggcgg cggggcgatc gacccgcgca caccggtccg
     7201 tctcgtgacc gactccgcgt tgtccgtgca cccgctcgaa gtcggcctgt cgctcaacct
     7261 cgcgttcggc ggctgcaacg cggcgctcgt cttctcgcgg tggcagggcg aatgagcgct
     7321 ccgcgcgcga tcggagtgac cggcgtcggc gtgct
//
LOCUS       DASPGN010000022         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_355622, whole genome shotgun sequence.
ACCESSION   DASPGN010000022 DASPGN010000000
VERSION     DASPGN010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355622"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /gene="thiC"
                     /locus_tag="VD788_00685"
     CDS             complement(<1..1705)
                     /gene="thiC"
                     /locus_tag="VD788_00685"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXV74805.1"
                     /translation="MRPIESPSPVWSRVYVSGERDGVRVPMREIRLTAPHGPVRLYDT
                     RGPYGDERHPVDLRSGLPALRRGWIRARGDVERLAGPSSRYRRERDADPGKDAIRFPE
                     PRAPYRARTGRIVTQLHYARRGEITPEMEFVALREGVEPELVRDEVAAGRAIIPANVN
                     HPESEPMVIGRRFLVKINANIGNSAVSSSIDEEVEKMVWAARWGADTVMDLSTGDDIH
                     ETREWIIRNAPLPIGTVPIYQALEKVGGVAEDLGWELYRDTLIEQAEQGVDYFTVHAG
                     VRLAHVPLTANRVTGIVSRGGAIMAKWCLAHHRESFLYTRFDEICEILAAYDVSFSLG
                     DGLRPGSIADANDRAQFAELETLGELTKTAWKHDCQVMIEGPGHVPMHLIKENMDRQL
                     ELCHEAPFYTLGPLTTDVAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPDKDD
                     VRQGIIAYKIAAHAADLAKGHPTARRWDDALSKARFEFRWEDQFNLSLDPDTARAYHD
                     ETLPADGAKVAHFCSMCGPKFCSMEITQQVRDYARAEGIDEGAARVRGMREKSREFRR
                     RG"
     regulatory      complement(1738..1849)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1896..2447)
                     /locus_tag="VD788_00690"
     CDS             complement(1896..2447)
                     /locus_tag="VD788_00690"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXV74806.1"
                     /translation="MDAARRIDRKKIYDGRVVRLSVDTVRLPNGKRAELELVRHRGAA
                     AIVPLDDDGQVLLVRQYRYAARRWLLEVPAGTLERQEPPERCARREVEEETGYRPSRI
                     VPLGWIFTTPGFTDEKIWLYLATDLSPTGARLDEDEVLTVERVPLADAVERAASGEIV
                     DAKSICALSRAARALGFRVATGS"
     gene            2523..>3639
                     /locus_tag="VD788_00695"
     CDS             2523..>3639
                     /locus_tag="VD788_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HXV74807.1"
                     /translation="MTHAVYLDYNATTPVAPEVADAVDRANRELWGNPSSVHEYGRAA
                     RRAVEQAREQVARLLGCAADEIVFTSGGTESDNAAIFGVAEALGTRGRHLVISAVEHA
                     AVDAACRSLEQRGYEVTRIGTDASGRVSAAEVEAALRPDTILVSLIHGQNETGVLQPV
                     EEVGRIARSRAIVFHTDTAQSVGKLAVDVDRLPCDLATVAGHKLYGPKGVGALYVRRA
                     TPFRGLLRGAAHEAGRRSGTENVGGIVGLGVACELATRELPARTRHLAALRERLSARL
                     CASVPDLVVHGAGAPRLPNTLSVAFPGATGAEVAARAAGVAVAAGAACHSGTAHVSRV
                     LTAMGVSRELALATLRLSVGCPTTEDDVDAAAESIARA"
BASE COUNT          505 a         1297 c         1256 g          581 t
ORIGIN      
        1 cgccgcgccg ccggaactcg cgtgactttt ctcgcatgcc acgcacccgg gcggccccct
       61 cgtcgattcc ctcggcgcgc gcgtagtcgc ggacctgttg cgtgatctcc atcgagcaga
      121 acttcgggcc gcacatcgaa cagaagtgtg cgaccttcgc cccgtcggcc ggcagcgtct
      181 cgtcgtggta agcccgggcg gtgtcggggt cgagcgacag gttgaactgg tcctcccagc
      241 ggaactcgaa gcgggccttg gacagcgcgt cgtcccagcg gcgcgcggtc gggtgtccct
      301 tggccagatc ggcggcgtgg gcggcgatct tgtaggcgat gatcccctgg cgcacgtcgt
      361 ccttgtcggg cagaccgaga tgctccttgg gggtcacgta gcacagcatc gcggtgccgt
      421 accaaccgat catcgccgcg ccgatcgccg acgtgatgtg gtcgtacccc ggcgcgacgt
      481 cggtcgtcaa cgggccgagc gtgtagaacg gcgcttcgtg gcacagctcg agctggcggt
      541 ccatgttctc cttgatcagg tgcatcggca cgtgacccgg gccctcgatc atcacctggc
      601 agtcgtgctt ccacgccgtc ttcgtcagct cgcccagcgt ctcgagctcg gcgaactgcg
      661 cgcggtcgtt cgcgtcggcg atcgagcccg gccgcaaccc gtcgccgagc gagaagctga
      721 cgtcgtaggc ggcgaggatt tcgcagatct cgtcgaaccg cgtgtagagg aagctctctc
      781 gatggtgggc gaggcaccac ttggccatga tcgccccacc gcgactgacg attccggtca
      841 cccggttcgc cgtcaggggt acgtgggcca gacggacacc cgcgtggacc gtgaagtagt
      901 cgacaccctg ctcggcctgc tcgatcaggg tgtcacgata gagctcccag ccgagatcct
      961 ccgcgacccc tccgaccttc tcgagcgcct ggtagatcgg cacggtcccg atcggcagcg
     1021 gagcgttgcg gatgatccac tcgcgcgtct cgtggatgtc gtcaccggtc gacaggtcca
     1081 tcacggtgtc ggcgccccag cgggcggccc acaccatttt ctccacctcc tcgtcgatcg
     1141 acgacgagac cgcggagttg ccgatgttcg cgttgatctt gaccagaaag cgtcggccga
     1201 tcaccatcgg ctcgctctcc gggtgattga cgttggccgg gatgatcgcc cgcccggccg
     1261 cgacctcgtc gcggacgagc tcgggctcga caccctctcg cagcgcgacg aactccatct
     1321 cgggcgtgat ctcgccgcgg cgcgcgtagt gcaactgggt gacgatgcgc cctgtgcgcg
     1381 cgcgatacgg ggcgcgcggc tccggaaacc ggatcgcgtc cttgcccgga tcggcatcgc
     1441 gctcgcgccg gtagcgggag ctcggtccgg ccagccgttc gacgtctccg cgcgcgcgga
     1501 tccaaccccg acgcagggcg ggcagcccgc ttcgcaggtc gaccggatgg cgctcgtcgc
     1561 cgtagggacc gcgggtgtcg tacaaacgga cgggcccgtg gggcgcggtc aaccggatct
     1621 ctcgcatcgg cacgcggact ccgtcacgct cgccggaaac gtagacgcgg ctccagacgg
     1681 gggaggggct ttcgatcggt ctcatcggtc ggctccttcg ttccagtacg acgggtggga
     1741 cggcaccgga gtgttgcctg cgctggcatt atccaggtca ggtcatagcg gtcggcagcg
     1801 cctcgccgcc ctctcagccc ggttacgccg agctcccgcg catcggaacc ccatggtagt
     1861 acccctgcgc cgtgcagcca agccacccgt tccgctcacg aaccggtcgc gacccgaaag
     1921 ccgagggccc gcgccgcgcg cgacaacgcg caaatcgact tggcatcgac gatctccccg
     1981 ctcgcggcac gctcgacggc gtccgccagg ggcacacgct cgacggtcag cacctcgtcc
     2041 tcgtccagcc gggcaccggt cggcgacagg tcggtcgcca gataaagcca gatcttctcg
     2101 tcggtgaagc ccggggtggt gaagatccag ccgagcggga cgatcctcga tggccggtaa
     2161 cccgtttcct cctcgacctc gcgccgggcg cagcgttcgg gtggctcctg ccgctcgagc
     2221 gttcccgccg gtacctccaa cagccatcgc ctcgccgcgt agcgatactg gcggaccagc
     2281 agtacctggc cgtcgtcgtc gagcggtacg atggccgcgg cgcctcggtg ccgaaccagc
     2341 tcgagctcgg cccgcttgcc gttcggcagg cgcacggtgt cgaccgacag tcgaacgacc
     2401 ctgccgtcgt agatcttctt tcggtcgatc cgtctggcgg cgtccatcgc tgctcctgtt
     2461 ccgggatccc gccgctcccg ccgacggtgc ggcataatgg cctcttgacc gggagaggcc
     2521 cgatgaccca cgcagtctac ctcgattaca acgcgacgac gccggtcgcg ccggaagtgg
     2581 ccgacgcggt cgatcgcgcg aatcgcgagc tgtggggcaa cccgtcctcg gtccacgagt
     2641 acggccgcgc cgcgcgccgg gcggtcgagc aggcccgcga gcaagtggcc cggctgctcg
     2701 gttgcgccgc cgacgagatc gttttcacga gcggcggcac cgaatcggac aacgcggcga
     2761 tcttcggcgt ggcggaggcg ctcggcaccc gcgggcgaca tctcgtgatc tcggcggtcg
     2821 agcacgcggc ggtcgacgcg gcctgccgat cgctcgagca gcgcggatac gaggtcacgc
     2881 ggatcggcac cgacgcgagc ggccgggtct ccgccgccga ggtcgaagcc gcgctgcggc
     2941 cggacacgat cctggtgtcg ctgatccacg ggcagaacga gaccggggtg ttgcagccgg
     3001 tggaggaggt cggtcgcatc gctcgctcgc gcgcgatcgt cttccacacc gataccgcgc
     3061 aatcggtcgg caagctggcg gtcgacgtcg accgattgcc gtgcgacctg gcgaccgtcg
     3121 ccggtcacaa actctacggg ccgaagggcg tcggcgcgct ctacgtgcga cgcgcgacgc
     3181 cgtttcgcgg tctgttgcgc ggcgcggcgc acgaggccgg gcgtcgctcg gggacggaga
     3241 acgtcggagg gatcgtcggg ttgggggtgg cgtgcgagct cgccacgcgc gagctacccg
     3301 cccgcacccg gcatctcgcc gcgctgcgag agcggctgtc cgcccgtctg tgcgcgtcgg
     3361 tcccggacct cgtggtccac ggggcgggcg cacctcgctt gccgaacacg ctgtccgtcg
     3421 cgtttcccgg agccaccggt gccgaagtcg ccgcccgcgc ggcgggcgtc gccgtcgctg
     3481 ccggcgccgc atgccattcc ggcacggcgc acgtctcccg cgtgctgacc gcgatgggcg
     3541 tgtcgcgcga gctcgcgctc gccacgctgc ggctgtccgt cggctgcccg acgaccgagg
     3601 acgacgtcga cgcggcggcc gagtcgatcg cgcgcgccg
//
LOCUS       DASPGN010000023         3127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_472681, whole genome shotgun sequence.
ACCESSION   DASPGN010000023 DASPGN010000000
VERSION     DASPGN010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472681"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1803
                     /locus_tag="VD788_00700"
     CDS             <1..1803
                     /locus_tag="VD788_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HXV74808.1"
                     /translation="VAAFVPSLPADPVIAGATIFWHLLLFGQLFFGAPPRRRQPARAW
                     VERADPVVDRSAPAVRHLLAVSVAISVAALGYRIGDRGTAQVICLLTGAAALGFAAPF
                     LVRRLGARSVAAFAIVAVLVAALAAAGDPGVALSLFALAQSCTLAWLWTRTGLFEDLL
                     DLFYRRPAHLVVASFLSLIALGSLFLSFPAASSGGTAVPLLDAVFTATSAACVTGLIV
                     LDTPADFSSFGHAVILVLIQAGGLNIMVLSAFAAGLLGRNLGFRGEGALRDMLALHPG
                     RSVRRLVLFIGLGTLAWESLGAAALSGLYLRQGVETGTAIWYGLFHAVSAFCNAGFSL
                     HSNSMVGFAADPAVLAVIATLITAGGLGFGVLGYAWTRLARRRRDAESLHARTVLLVS
                     LALVVSGAVVYCVVEWNHTLADLSPVDRVANALFQSVTLRTAGFNSVEFERLRPATLF
                     MMLAWMVVGASPGGTGGGIKTTTAAVLIGSVLALLSRRERAVLFRRRISLDTVFRSGA
                     IATAAAVAVAAGAFALLATQGQRFEVLLFEAVSAFGTVGLSLGATPSLDGTGKVIVMC
                     LMFAGRIGPLTLALLVGRASASRVSYPDSRIMVG"
     gene            1816..2472
                     /locus_tag="VD788_00705"
     CDS             1816..2472
                     /locus_tag="VD788_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HXV74809.1"
                     /translation="MARQFAVIGLGAFGRQVAIQLERLGHEVLAIDLDERLVNAVAEE
                     IEASVRADATDESALREMHVDGVSCAVVAIGAEAIEASILATTLLRQIGVPQIVARAV
                     NPLHARALRAVGAHAVVRPEEEMGERLARRLAQPNVLERIELGRDAELAEVPVPGEFV
                     GKSLIDLEVRRRHAVTVVAIRRGDRILATLEGSERMEAGDVLVVIGAPAAIEKLASRA
                     "
     gene            2476..>3127
                     /locus_tag="VD788_00710"
     CDS             2476..>3127
                     /locus_tag="VD788_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74810.1"
                     /translation="MKLHTRLLLAYGYLVTLVLVGAAGAALGFFSLGNRIGAVLDDNF
                     ESVRASMEMLEALEHQQNAVLTALLETARAPGDVESSERAFLGALARARRNVTEKQER
                     PALETIAARYDDYREARQRLLAEPPERPFAAYRSECVPLIESVQEGVRRLLELNHGAM
                     VQADRDAQATAARRAIGYAAMTAVALLSMGWLARGLRVNLVARLEDLRAVAQAIGRG"
BASE COUNT          363 a         1052 c         1150 g          562 t
ORIGIN      
        1 gtcgcggcct tcgttccctc gctgccggcg gatccggtga tcgccggcgc cacgatcttc
       61 tggcacctgc tgctgttcgg ccagctgttc ttcggagcac cgcctcgacg tcgtcaaccg
      121 gcccgcgctt gggtcgagcg cgccgacccg gtcgtcgatc gcagcgcgcc ggcggtgcga
      181 cacctgctcg cggtctccgt cgcgatctcg gtcgccgcgc tcggctatcg catcggcgat
      241 cgcgggacgg cgcaggtcat ctgtctgttg acgggcgccg cggcgctcgg tttcgcggca
      301 ccgtttctcg tccggcgtct gggcgcccgc tcggttgccg cgttcgcgat cgttgcggtc
      361 ctcgtcgccg cgctggcggc ggccggcgat cccggtgtcg ccctgtcgct attcgcactg
      421 gcccagagct gtacgctggc gtggctttgg acgcgaacgg ggctgttcga ggacctgttg
      481 gacctgttct accgacgccc cgcgcacctc gtcgtcgctt cgttcctgtc gttgatcgcg
      541 ctcggatcgc ttttcctcag cttccccgcg gcctcctccg gcggaacggc cgtgcccctg
      601 ctcgacgcgg tcttcaccgc aaccagcgcc gcgtgcgtga ccggcctgat cgtgctcgac
      661 acaccggctg acttttcgtc gttcggccac gcggtgatcc tggtgttgat ccaggccggc
      721 gggctgaaca tcatggtgct gtcggccttc gccgccggcc tgctgggccg caacctcggc
      781 ttccgcggcg agggggcgtt gcgcgacatg ctcgccctcc atcccggacg gtccgtccgc
      841 cggctcgtgc tgttcatcgg cctcggcacc ctggcgtggg agtcgctcgg ggcagcggcg
      901 ctgtcgggcc tctacctgcg gcagggcgtc gagacgggga cggcgatctg gtacgggctg
      961 ttccacgcgg tgtcggcctt ctgcaacgcc ggcttctcgt tgcactcgaa ctcgatggtc
     1021 gggttcgccg ccgaccccgc ggtgctggcg gtgatcgcga cgttgatcac cgccggcggg
     1081 ctgggcttcg gcgtgctcgg ctacgcatgg acgcgactgg cccgccgtcg ccgggacgcc
     1141 gagtcgctcc acgcccggac ggtgctcctc gtttcgctcg cgctggtggt gagcggggcg
     1201 gtcgtctact gcgtcgtcga gtggaaccat acgctggccg atctgtcccc ggtcgaccgc
     1261 gtggccaacg cgctgttcca gagcgtgacg ctgcgcacgg ccggcttcaa ctcggtcgag
     1321 ttcgagcggc tccggccggc gacgctgttc atgatgctgg catggatggt cgtcggcgcg
     1381 tcccccggtg gcaccggcgg tgggatcaag accacgacgg cggccgttct gatcggctcg
     1441 gtgctcgccc tgctgtcgcg gcgcgagcgg gcggtgttgt tccgtcggcg gatctcgctc
     1501 gacacggtct tccgcagtgg tgcgatcgcc accgccgcgg cggtcgccgt cgcggccggc
     1561 gcgttcgccc tgctcgcgac acaggggcag cgattcgagg tcctgctgtt cgaagcggtc
     1621 agtgcgttcg gtacggtcgg gctgtcgctc ggtgcgacgc cgagtctcga cggaaccggg
     1681 aaggtcatcg tgatgtgcct gatgttcgcc gggcggatcg gcccgctgac gctcgcgctg
     1741 ttggtcgggc gcgcgtccgc cagccgcgtg agctaccccg actcgcggat catggtcggt
     1801 tgatcggaga gagtgatggc gagacagttc gcagtgatcg gtctgggcgc gttcggccgt
     1861 caggtggcga tccagctcga gcggctcggg cacgaggtat tggcgatcga tctcgacgag
     1921 cgactggtga acgcggtcgc cgaggaaatc gaggcgtcgg tgcgtgcgga tgcgaccgac
     1981 gagagcgcgc tgcgcgagat gcacgtcgac ggcgtctcgt gtgcggtcgt cgcgatcggt
     2041 gcggaggcga tcgaagcgtc gatcctggcg acgacgctgt tgcggcagat cggcgtcccg
     2101 cagatcgtcg cccgcgcggt caacccgctg cacgcgcgcg cgctgcgcgc ggtcggcgcg
     2161 cacgccgtcg tccgtcccga agaggagatg ggcgaacggc tggcgcgccg tctggcgcag
     2221 cccaacgtgc tggagcgcat cgagctcggc cgtgacgcgg agctggccga ggtacccgtt
     2281 cccggcgagt tcgtcggcaa gtcgttgatc gacctcgagg ttcgccgccg gcacgcggtc
     2341 accgtcgtcg cgatccggcg cggggatcgg atcttggcca cgctcgaagg ctccgagcga
     2401 atggaagccg gcgatgtcct cgtcgtaatc ggcgctccgg cggcgatcga gaagctcgcc
     2461 tcgagagcct gagcgatgaa actgcacacg cggctgttgt tggcatacgg ctacctggtg
     2521 acgctggtgc tggtcggcgc cgcgggggcc gcgctcggct tcttcagcct cggtaaccgc
     2581 atcggcgcgg tgctggatga caacttcgag agtgtgcggg cctcgatgga gatgctcgaa
     2641 gcgctggagc atcagcaaaa cgccgtgttg accgcgctgc tcgaaaccgc ccgggcgccc
     2701 ggtgacgtcg agtcctccga gcgggcgttt ctcggcgcgc tggcccgggc tcggcgcaac
     2761 gtcaccgaga aacaggagcg gccggcgctc gagacgatcg ccgcgcggta cgacgactat
     2821 cgcgaggcgc gacagcggtt gctcgccgaa ccgcccgagc ggccgttcgc cgcctaccgc
     2881 agcgagtgcg tgccgttgat cgagtcggtc caggaaggag tgcgccggct gttggagctg
     2941 aaccacgggg cgatggtgca ggccgatcgc gacgcgcagg cgaccgccgc gcgccgggcg
     3001 atcggctacg ccgcgatgac cgccgtcgcg ttgctgtcga tgggctggct cgcccgcggt
     3061 ctgcgggtga acctggttgc gcgtctcgag gatctgcgcg cggtggccca agcgatcggt
     3121 cgcgggg
//
LOCUS       DASPGN010000024         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_239109, whole genome shotgun sequence.
ACCESSION   DASPGN010000024 DASPGN010000000
VERSION     DASPGN010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239109"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VD788_00715"
     CDS             complement(<1..1216)
                     /locus_tag="VD788_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXV74811.1"
                     /translation="MDHGSPTGHPDPEGELRLERRVAELEQLLDNVDGIVWTGDPGGL
                     RFRYVSRRAERLLGYPVEQWIEEPQFWLNHMHPDDREWVPSLAYSQSRVGQAHEFEFR
                     MIAADGRTIWLRNVVTVAKQEEGGPVLRGLMIDISARKESERRTERRNAALHELARSE
                     ALDSGDFDLFTRLAIQTAADVLDVDRIGVWLFNDDRSILRCESLYERGKDRFSTGLEL
                     QAAAYPRYFAALADGRFVAADDALTDPTTSEFAESYLAPLGITSMLDAPIRRGQKLIG
                     VVCHEHVGPARRWLHDEREFAASIAHFVSHALEARHRSRAEQDVRRGQQRLLEQKLLE
                     KERVERELARTREHLVRQTRLSTIGQIAASIAHELRNPLGAIRNATFYLRRHVPREDP
                     KWVKHVGIIEQEV"
     gene            1351..1584
                     /locus_tag="VD788_00720"
     CDS             1351..1584
                     /locus_tag="VD788_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74812.1"
                     /translation="MPRHPHQYDVPDPATELQRLAQRVASMIVTSSYSDLECALAERE
                     LRMECLRLLPDCMDLFDMVYVSRFQRLREQFRH"
     gene            complement(1544..2482)
                     /locus_tag="VD788_00725"
     CDS             complement(1544..2482)
                     /locus_tag="VD788_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74813.1"
                     /translation="MRRRIRLPAIALFLATGAGAAAAERYEIRFNRPMAVGDRFLISA
                     TGSLTNTTTMIRDEAVVRHDDLSIRIELQALIEILEVDERGRPLRESLGVDFLVYGAG
                     DAPTQALPTGSVIIAETVGSRTDFRLEHGELPAMARAALQIVVGTYTGDVPDDDLIFG
                     TTEPQPVGGSWGVDAAAFARAMRANGVVLGSDGLDGTVELVGVERIEGVECLRVEAVV
                     KVDGLATRDVAQGIEQLHGSAEVELTGLFPTDLGRPRMHDSKTVGMELVVKSTAGPMT
                     GTTMRSTSLRQGKKTMIPFARRQVSDETAPASAGSD"
     gene            2592..3476
                     /locus_tag="VD788_00730"
     CDS             2592..3476
                     /locus_tag="VD788_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXV74814.1"
                     /translation="MNKTLIVCNPISGGGRGRKRGVQLEQRLRLAGREFESFVTERAG
                     DARRRAATVEADVDRIVVIGGDGTLNEVLNGLRDPSRVPITQLATGTANLLAHDLRLP
                     FRAEGMGRLLDDGSIRYVDMGQAGDRRFLLVVSAGFDAMVTHEAQRGRSGPSGYLSWI
                     VPTIRTLRGYRPPNLEVKVDGGPSVRGELVIVSNVRNYGGIFTVADRASCDSGHLDVC
                     VLKRASLPGLARAAFSGLTGGLSSSPGVVYLTGRRIELDADRPVPVELDGDPFGTTPV
                     EIELTPRCVPVVVPAFPV"
     gene            complement(3473..>3815)
                     /locus_tag="VD788_00735"
     CDS             complement(3473..>3815)
                     /locus_tag="VD788_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74815.1"
                     /translation="VVSYDGTVGGKSDARVVSLDIEFDAADEPPRAASELLARLRRAA
                     PGPDLIGRSPDEATSWIDRDCTVRVETSRRAPDWWAPTDEARYHVRIESIDPSGDALV
                     GRDRRGAGTPD"
BASE COUNT          560 a         1329 c         1304 g          622 t
ORIGIN      
        1 ccacctcctg ctcgatgatc cccacgtgct tgacccactt cgggtcctcg cgcggcacgt
       61 gtcgccgcag gtagaacgtc gcgttgcgga tcgcgccgag cgggttgcgc agctcgtggg
      121 cgatgctggc cgcgatctgc ccgatcgtgc tcaggcgtgt ctgacgaacg agatgttcgc
      181 gggttcgggc gagctcgcgc tccacccgtt ccttctccag cagtttctgc tccagcagtc
      241 gttgctggcc gcgccgcacg tcctgctcgg cacggctccg atggcgcgcc tcgagcgcgt
      301 gagagacgaa atgggcgatc gacgcggcga actcccgctc atcgtgaagc cagcgacgcg
      361 ccgggccgac gtgctcgtgg cagaccacgc cgatcagctt ctgaccgcgt cggatcggcg
      421 cgtccagcat cgaggtgatt ccgagcgggg ccaggtagct ctcggcgaac tcgctggtcg
      481 tcggatcggt cagcgcgtcg tcggcggcga cgaacctgcc gtcggccagt gcggcgaagt
      541 agcgtgggta ggcggcggcc tgcagctcga gaccggtcga gaagcggtcc ttgccgcgct
      601 cgtacaggct ctcgcagcgc aggatcgatc gatcgtcgtt gaacagccac accccgatgc
      661 ggtcgacgtc gagcacgtcc gcggcggttt ggatcgccag ccgggtgaac aggtcgaaat
      721 cgccggaatc gagcgcctcg ctgcgtgcca gctcatgcag cgcggcgttg cgtcgctcgg
      781 tccgccgctc ggattccttg cgcgccgaga tgtcgatcat cagcccgcgg agcacgggcc
      841 cgccctcttc ttgcttcgcg actgtaacga cgttgcgcaa ccaaatggtg cgaccgtcgg
      901 cggcgatcat gcggaactcg aactcgtgag cctgacctac acgactctgc gagtaggcga
      961 gactcggtac ccattcccgg tcgtcggggt gcatgtggtt cagccagaac tgcggttcct
     1021 cgatccactg ttcgaccggg tagccgagca aacgctcggc gcggcggctg acgtagcgga
     1081 agcgcaatcc gccggggtcg ccggtccaga cgatcccgtc gacgttgtcg agcagctgct
     1141 ccagttcggc gacccgtcgc tccagacgca attcaccctc ggggtccgga tgaccggtcg
     1201 gggagccgtg gtccatgacc caccaccttc gcgggactgg ttcgtgtggg aatcgtacca
     1261 ccgaccgccc tgccaccgaa gcaccgcgcg tgaccgggct ggcacctccc gccggctcgc
     1321 cttagattct gagcgaggga ggtcggacgc ttgccgcgtc atccgcacca gtacgatgtc
     1381 cccgatccgg ccaccgagct ccagcgtctg gcgcagcggg tggcctcgat gatcgtcacc
     1441 tcgagctact cggacctcga gtgcgcgttg gccgagcgcg agctgcggat ggaatgcctg
     1501 cggctgctcc ccgactgcat ggacctgttc gacatggtct acgtcagtcg cttccagcgc
     1561 ttgcgggagc agtttcgtca ctgacctgcc ggcgggcgaa cgggatcatc gtcttcttcc
     1621 cctgacgcaa cgaggtcgaa cgcatcgtcg tcccggtcat cggcccggcg gtgctcttga
     1681 ccaccagctc catgccgacc gttttgctgt cgtgcatgcg cggtcggccg aggtcggtcg
     1741 ggaagagccc ggtcagctcg acctcggccg acccgtgcag ctgctcgatc ccctgcgcga
     1801 cgtcgcgggt cgccaggccg tcgaccttga ccacggcctc gacccgcagg cactcgacac
     1861 cctcgatccg ctcgaccccc accagctcga ccgtcccgtc gagaccgtcg ctgccgagca
     1921 ccacgccgtt ggcgcgcatc gcgcgggcga acgccgcggc gtcgacgccc cagctcccgc
     1981 cgaccggctg cggctcggtc gtgccgaaga tcaggtcgtc gtcggggacg tcgccggtgt
     2041 aggtaccgac cacgatctgc agcgccgcgc gggccatcgc cggcagctcg ccgtgctcga
     2101 ggcgaaagtc ggtccgcgag ccgaccgttt cggcgatgat caccgacccg gtgggcaacg
     2161 cctgcgtcgg cgcgtcaccg gccccgtaga cgaggaagtc gacgccgagc gactctctga
     2221 gcggccgccc gcgttcgtcc acctcgagga tctcgatcag cgcctggagc tcgatccgaa
     2281 tcgaaagatc gtcgtgacgc acgaccgctt cgtcgcggat catcgtcgtc gtgttggtca
     2341 ggctgccggt ggccgagatc aggaagcggt cgcccacggc catcgggcgg ttgaagcgga
     2401 tttcgtagcg ttccgcggcc gccgcacccg cgccggtcgc gaggaacaag gcgatcgccg
     2461 gcaatcggat ccttcgtcgc accacacctc ccggccgcaa ccaccgatcg agtcggacag
     2521 gttacctcaa gccaccggcg gattcgacac gccccgctcc gtcgacgggt tccgcgctag
     2581 aatcgcgcgc ggtgaacaaa acgctgatcg tctgcaaccc gatttcgggc ggtggtcgcg
     2641 ggcgcaaacg cggtgtgcag ctcgagcagc ggctgcggct cgccggccgg gaattcgagt
     2701 cgttcgtgac cgaacgggcc ggtgacgcgc gccgccgcgc cgccacggtc gaggcggatg
     2761 tcgatcggat cgtcgtcatc ggcggagacg gcacgttgaa cgaagtgctg aacgggctgc
     2821 gcgacccatc gcgcgtgccg atcacacaac tcgcgaccgg caccgcgaat ctgctggcgc
     2881 acgacctgcg gctgccattc cgcgccgaag gcatgggccg cttgctcgac gacggatcga
     2941 tccgctatgt cgacatggga caggccgggg accgacggtt cctgctcgtc gtcagcgccg
     3001 ggttcgacgc gatggtgacg cacgaagcac agcgcgggcg gtccgggccg tcgggctatt
     3061 tgagctggat cgtcccgacg atccgcacgc ttcgcggcta ccggccgccg aacctcgagg
     3121 tcaaggtcga cggcggcccg tcggtccgcg gcgagttggt gatcgtcagc aacgtacgga
     3181 actacggcgg gatcttcacc gtcgcggacc gcgcgagctg cgactcgggt cacctggacg
     3241 tctgcgtgct gaagcgtgcg tcgttgcccg ggctggcgcg ggccgcgttc tcggggttga
     3301 ccggcggtct ctcgtcgagt ccgggcgtgg tctacctgac tggccgacgc atcgagctgg
     3361 acgccgaccg gccggtaccg gtcgagctcg acggcgaccc gttcggcacg accccggtcg
     3421 agatcgagtt gacgccgcgc tgcgtgccgg tcgtggtacc cgccttcccg gtctagtccg
     3481 gcgtgccggc gccgcggcga tcgcgcccga ccagcgcatc gccggacggg tcgatcgact
     3541 cgatgcggac gtggtagcgc gcctcgtcgg tcggcgccca ccagtcgggg gcacgacgag
     3601 aggtttcgac ccgcaccgtg caatcgcgat cgatccagga ggtcgcctca tccggcgagc
     3661 gcccgatcag gtcgggaccg ggcgcggcac ggcggagacg tgcgagcaac tcgctcgcgg
     3721 ctcgcggcgg ctcgtcggcc gcgtcgaact cgatgtcgag cgagacgacg cgcgcgtcgc
     3781 tcttgccgcc gaccgtgccg tcgtaggaga cgacg
//
LOCUS       DASPGN010000025         7462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_707508, whole genome shotgun sequence.
ACCESSION   DASPGN010000025 DASPGN010000000
VERSION     DASPGN010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707508"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VD788_00740"
     CDS             complement(<1..543)
                     /locus_tag="VD788_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74816.1"
                     /translation="MGSPDDGGRIERLDRESLEARVRQAAERLVPDRVIDDLEIRHLT
                     HVITECSLLLADVYSDERLRRIMRPPTELIAGLIDQLAREQATDAGRVLQRVRHLPDD
                     VRVVGDKALFDLGLLGRREVKGYDLVDLGSRAYRSAGEALELLAEDRQLRDFFKKNRL
                     LMLPLEEEVVFLRQCSAQFEM"
     gene            792..1079
                     /locus_tag="VD788_00745"
     CDS             792..1079
                     /locus_tag="VD788_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXV74817.1"
                     /translation="MKVKPLYDRLLVTRVDEKEVKQGGIIIPDSAKEKPMEGKVVAVG
                     SGRVEKDGKTVPMQVKVGDRILFGKYAGTEIKIEDKEHVILREDEVLGIIQ"
     gene            1185..2822
                     /gene="groL"
                     /locus_tag="VD788_00750"
     CDS             1185..2822
                     /gene="groL"
                     /locus_tag="VD788_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXV74818.1"
                     /translation="MAAKTIAYAEDARQAMLRGVNRLADAVKITLGPKGRNVVLEKKF
                     GSPLSTKDGVSVAKEIELENPNENMGAQLVREVASKTSDVAGDGTTTATVLAQAIFRE
                     GVKAVTAGGNPMDIKRGIDLAVESAVDAIKKSSRPVAGKAIAQVGSISANSDEMIGKI
                     IAEAMDKVGKDGVITVEEAKGLETSLEVVEGMQFDRGYLSPYFVTDSERMEAVLENVN
                     ILIHEKKISSMKDLLPILEQIAKTGKPLVIIAEDIEGEALATLVVNKLRGTLHVAAVK
                     APGFGDRRKAMLQDIATLTGGTCITEDLGIKLENIKLTDLGKAKKVVIDKDNTTIVEG
                     GGKAKDIAGRVAQIRNQIEETTSDYDREKLQERLAKLVGGVAVIKVGAATESEMKEKK
                     ARVEDAMHATKAAVEEGIVAGGGVALLRAQKGLDKLVKTEKNEDRQMGIRIVQRSLEE
                     PIRQIANNAGMEGSVVVNEVRGLDETKGFDAQNEKYTDMINAGIIDPTKVVRSALQNA
                     ASIAGLMLTTEALVSEVPEDEKKSAPAMPGGDMGGGF"
     gene            2955..6260
                     /locus_tag="VD788_00755"
     CDS             2955..6260
                     /locus_tag="VD788_00755"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74819.1"
                     /translation="MHARRPLVPLVCLLLLSAPVLAKKDKDKPEGEGPFKTSTFAGLA
                     FRGIGPAVTSGRISDIAVDPTDPARYFLAVASGGVWRTVNSGTTWEPVFDDQGSYSIG
                     CVTIDPHDPLTVWVGTGENNSQRSVSYGDGVYRSTDGGTRWTRMGLELSEHIAKIVVD
                     PRTSDTVWVAAQGPLWAPGGERGLYKSTDGGASWQLSLEIGENTGVTDVVLDPRNPDV
                     VYAAAYQRRRHVWTLIDGGPESAIFKSTDGGASWTKLTNGLPEADLGRIGLAVSPADP
                     DVVYAIVEAERDEGGFFRSTDAGGNWTKRSEYVSDSPQYYQEIVADPRDPDRVYSLDT
                     WMMVSEDGGENFRRVGNRSKHVDDHALWIDPHNTDHLRAGCDGGLYESWDRGANWAFT
                     ANLPLTQFYRVTPDNDWPFYNVYGGTQDNFTLGGPARTISLNGIANGDWIVTLGGDGF
                     EPQIDPDNPDIVYSQYQYGGLSRFDRRSGERIDIQPQVGPDEDPLRWNWDSALIISPH
                     SSTRLYFAAQRVFRSDDRGDHWRPISGDLTRQLARDSLEVMGRVQKADAVAKGASTSP
                     YGNIVALSESARLEGLIYAGTDDGLVQLLEPETGEWRAASGIDGVPELSYVSDLQTSL
                     HRTDTVFAALDNHKSGDFAPYLFRSDDRGKSWKSIVGDLPARGTVYTVVEDDVRPELL
                     FAGTEFGVYFTIDGGRRWIRLKGGIPVIAVRDLEIQRRETDLVVGTFGRGIYILDDYS
                     PLRRIDAGLLERPAALFAPRESWMFMPSLPLGLPAKSFQGDDYYAAPNPAFGAVLTYY
                     LKDGLETRAERRRRTEKETEQQGRPYDYPEDEELRAEMLEHDPAVLLTIRDAEGRVVR
                     RLDGPVTAGIHRIAWDLRFPPSTPVRLDAGDPDPFDPPPVGPMVLPGRYTVELATVVD
                     GVVDTLGAPETIVATPLGLATLAAGDRAGLLEFQRRTADLQRAVLGAVRVVDETERRI
                     DHLRRAIHDTPGADPDLAVELRAIENRLAALTIGLSGDPLIRRYNNPAPRSVVDRVER
                     IVSSQWTSTAPPTGTNLDAYRIAGEGFAPLLEELRAIVEGDLPRIERRLDQVGAPWTP
                     GRVPRWSMDDARP"
     gene            6461..>7462
                     /locus_tag="VD788_00760"
     CDS             6461..>7462
                     /locus_tag="VD788_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74820.1"
                     /translation="MAIFVLTLLGLVGVALLHLTSTEMTMSKRALDLKQAFYLAEAGE
                     ERARTTLYLADTNQDFSDELASYAGANGVFDLDVSNLRLVFDGAGAVTGVTGVGDDTP
                     VHAITAFASGFYAAYVTNDPAEGITTTTDANDLVMITAVGAGRDRAVEVVQAIVERWQ
                     IVPQLPPATITLLGDQPLFDGGTSDPHDYSGEDCPGGIPNFNVPTVGTIGSDAELQSE
                     LGIVEPDGPDYDSGPFSQQETFVDLTNLADPMLVANGFTGLDPMWTDCEALHDLVEEL
                     RKIADVECSAGTACTIPSQLTSNVIFVNGDYEINPPGGAGTLVVTGELEYDGRAGWEG
                     "
BASE COUNT         1393 a         2459 c         2525 g         1085 t
ORIGIN      
        1 catctcgaac tgcgcggagc attgccggag gaagacgacc tcctcctcca gcggcagcat
       61 caacaaccgg ttcttcttga agaaatcgcg cagctgccgg tcctcggcca gcagctcgag
      121 cgcctcgccg gccgagcggt aggcccggct gccgagatcg accagatcgt aacccttgac
      181 ctcgcgacgg ccgagcagcc ccagatcgaa cagcgccttg tcgcccacca cgcgaacgtc
      241 gtccggcagg tgtcggaccc gctgcagtac cctccccgca tcggtcgcct gctcgcgggc
      301 caactgatcg atcagtccgg cgatcagctc ggtcggcggt cgcatgatcc gacgcagccg
      361 ttcatcggag tagacgtccg ccaacaacaa ggagcattcg gtgattacgt gggtcaggtg
      421 gcggatttcc aggtcatcga tgacccggtc cgggacgagt cgttccgcgg cttgccgtac
      481 ccgggcctcg agcgattcac ggtcgaggcg ttcgatcctg ccaccgtcgt cgggagagcc
      541 catggcgcag ctccgtcgtg ccccaagcct tcccgttaag atggcgccaa tccgagggaa
      601 tggccacgag aaccgaagga aagatgcggc aagcggcccg ctcgacgcgc tcgacgagat
      661 tcttccgggg gtcccttgac accctgtcgg gggaatccta tagtgttggc actctggcgc
      721 ctagagtgcc aggatagtca gcgtaaaccc aaacaaaata acaacttagt tcaagaaaag
      781 gaggaagcac gatgaaggta aagccgctct acgaccgctt gctggttacc cgcgtcgacg
      841 agaaggaggt gaagcaaggc ggcatcatca tccccgacag cgcgaaagag aagccgatgg
      901 aaggcaaggt cgtcgcggtc ggatccggcc gggtggaaaa ggacggcaag accgtcccga
      961 tgcaggtcaa agtcggcgac cgcatcctgt tcggcaagta cgccggcacc gagatcaaga
     1021 tcgaggacaa agagcacgtc attctgcgtg aggacgaggt gctcggcatc atccagtgag
     1081 cgtctgacgc gcccgcgggc gcgttttttc tgcccgccgg acggaacggc cgttcggttc
     1141 acgccgggca accagaatcc gattttcgag gaggaacgtc agaaatggct gcaaagacca
     1201 ttgcctacgc tgaagacgcc cgccaagcga tgctgcgcgg ggtcaaccgc ttggccgacg
     1261 cggtgaagat caccctgggt ccgaagggcc gcaacgtggt gctggagaag aagttcggct
     1321 cgccgttgtc caccaaggac ggcgtctccg tggcgaagga gatcgagctc gaaaacccga
     1381 acgagaacat gggcgcccaa ctcgtgcgcg aggtcgcgag caagacctcg gacgtcgccg
     1441 gtgacggcac gacgacggcg acggtgctgg cccaggccat cttccgcgag ggcgtgaagg
     1501 ccgtcacggc cggcggcaac ccgatggaca tcaagcgcgg gatcgacctg gccgtcgagt
     1561 cggcggtcga tgcgatcaag aagagctcgc gtcccgtcgc gggcaaggcg atcgcgcagg
     1621 tcggcagcat ctcggccaac agcgacgaga tgatcggcaa gatcatcgcc gaggcgatgg
     1681 acaaggtcgg caaggacggc gtgatcaccg tcgaagaggc caagggtctc gaaacctcgc
     1741 tcgaggtggt cgaaggcatg cagttcgacc gcgggtatct gtcgccctac ttcgtcaccg
     1801 attccgagcg gatggaagcc gtgctcgaga acgtcaacat cctgatccac gagaaaaaga
     1861 tctcgtcgat gaaggacctg ttgccgatcc tcgaacagat cgccaagacc ggcaagccgc
     1921 tggtgatcat cgccgaggac atcgagggtg aggcgctggc cacgctggtc gtcaacaagc
     1981 tgcgcggcac gttgcacgtc gcggcagtca aggctcccgg cttcggcgat cgtcgcaagg
     2041 cgatgctgca ggacatcgcc accctgaccg gcggtacctg cattaccgag gacctgggca
     2101 tcaagctcga gaacatcaag ctgaccgacc tgggcaaggc gaagaaggtc gtcatcgaca
     2161 aggacaacac gacgatcgtc gagggcggcg gcaaggccaa ggacatcgcc ggtcgcgttg
     2221 cgcagatccg caatcagatc gaggagacga cctccgacta cgatcgcgag aagctgcagg
     2281 agcgtctggc gaagctggtc ggcggcgtcg cggtgatcaa ggtcggtgcg gcgaccgaat
     2341 cggagatgaa ggaaaagaag gcccgggtcg aggacgcgat gcacgcgacc aaggccgcgg
     2401 tcgaagaggg catcgtcgcc ggcggcggcg tggcgctgtt gcgcgcgcag aaaggcctcg
     2461 acaagctggt caagaccgag aagaacgaag accggcagat gggcatccgc atcgtgcagc
     2521 ggtcgctcga ggagccgatc cggcagatcg cgaacaacgc cggcatggaa ggctcggtcg
     2581 tggtcaacga ggtccgcggt ctcgacgaga ccaaaggctt cgacgcacag aatgaaaagt
     2641 acaccgacat gatcaacgcg ggaatcatcg acccgaccaa ggtcgtccgc tcggcgttgc
     2701 agaacgccgc gtcgatcgcc ggcctgatgc tgacgaccga ggcgctcgtc agcgaggtac
     2761 ccgaggacga gaagaagagc gcgccggcga tgcccggtgg cgacatgggc ggcggcttct
     2821 gattccattc gctcgcggtt gcacgttcga gccaagcccg gcccccgcgg ccgggctttt
     2881 ttcttggccg gcgggctggg ctaagatcgc acgcccgcgg agcagacgcg ctccgccgga
     2941 ttcggaggtc gccgatgcac gcccgccgtc cgctcgtccc gctggtctgt ctgctgctgc
     3001 tgtccgcgcc ggtgctcgcc aagaaggaca aggacaagcc ggagggcgag ggtccgttca
     3061 agacgtcgac cttcgccggg ctcgcgttcc gcggtatcgg cccggcggtg acttcgggcc
     3121 gaatctcgga catcgccgtc gacccgaccg accccgcgcg ctacttcctc gcggtcgcct
     3181 cgggcggcgt gtggcgcacg gtcaattccg ggacgacctg ggaaccggtc ttcgacgacc
     3241 agggctcgta ctcgatcggc tgcgtgacga tcgacccgca cgatccgctg acggtctggg
     3301 tcggcaccgg cgagaacaac agccagcgca gcgtctccta cggtgacggg gtctaccgct
     3361 cgaccgacgg cggcacgcgc tggacgcgaa tgggtctcga actctccgag cacatcgcca
     3421 agatcgtggt cgatccgcgc acgtccgata cggtctgggt ggcggcccag ggcccgctgt
     3481 gggcgccggg cggcgagcgg ggactgtaca agtcgaccga cgggggcgcg agctggcagt
     3541 tgtcgctcga gatcggcgag aacaccggtg tgaccgacgt ggtgctcgac ccgcgcaacc
     3601 cggacgtggt ctacgccgcg gcctaccagc gacgccggca cgtctggacc ttgatcgacg
     3661 gaggccccga atcggcgatc ttcaaatcga ccgacggggg cgcgagctgg accaaactga
     3721 cgaacggtct gcccgaggcc gacctgggaa ggatcggact cgcggtctca cccgccgatc
     3781 ccgacgtcgt ctatgccatc gtcgaggccg agcgcgacga gggcggtttc ttccgatcga
     3841 ccgacgccgg aggcaactgg accaagcgca gcgaatacgt cagcgacagc ccccagtatt
     3901 accaggagat cgtcgccgac ccgcgcgacc ccgaccgcgt ttactcgctc gatacgtgga
     3961 tgatggtcag cgaggacggc ggcgagaact tccgccgggt gggcaaccgc tcgaagcacg
     4021 tcgacgacca cgcgttgtgg atcgatccgc acaataccga ccacctgcgc gccggctgcg
     4081 acggcggtct gtacgagagc tgggatcggg gagccaattg ggcctttacc gccaatctcc
     4141 cgctgaccca gttctatcgc gtcactcccg acaacgactg gccgttctac aacgtctacg
     4201 gcggcaccca ggacaacttc acgctcggcg ggccggcgcg cacgatcagc ctcaacggga
     4261 tcgccaacgg ggactggatc gtcacgctcg ggggcgacgg gttcgagccg cagatcgacc
     4321 cggacaaccc cgacatcgtc tactcgcagt accagtacgg cggcttgtca cggttcgacc
     4381 gaaggagcgg cgagcgcatc gacatccagc cgcaggtcgg cccggacgag gacccgctgc
     4441 gttggaactg ggattcggcg ctgatcatca gcccgcactc ttccacgcgg ctctacttcg
     4501 cggcgcagcg cgtttttcgc agtgacgatc gcggcgacca ctggcgtccg atcagcggcg
     4561 atctgacccg gcagctggcg cgcgactcgc tcgaggtgat gggacgggtc cagaaggcgg
     4621 acgccgtcgc caagggtgcg tcgacgtcgc cgtacggcaa catcgtggcg ctcagcgagt
     4681 cggcgcggct cgaagggctg atctacgcgg gcaccgacga cggcctggtc cagctgctcg
     4741 agcccgagac cggcgaatgg cgcgcggcca gcgggatcga cggcgtgccg gagctgagtt
     4801 acgtgagcga cctccagacg tcgctgcacc gaaccgacac cgtcttcgcc gcgctcgaca
     4861 accacaagag cggcgatttc gcgccgtatc tttttcgcag cgacgaccgc ggcaagagct
     4921 ggaagtcgat cgtgggcgat cttcccgcgc ggggcacggt ctacaccgtg gtcgaggacg
     4981 acgtccggcc ggagctgctg ttcgccggta ccgagttcgg tgtctacttc acgatcgacg
     5041 gtggtcggcg ctggatccgc ttgaagggcg gcataccggt catcgcggtg cgcgacctcg
     5101 agatccaacg gcgcgagacc gatctggtgg tcggcacctt cggccggggc atctacatcc
     5161 tcgacgatta ctcgccgctg cggcggatcg acgccgggtt gctcgagcgg ccggcagcgc
     5221 tgttcgcccc acgcgagagc tggatgttca tgccgtcgct gccgctcggg ctgccggcca
     5281 agtcgttcca gggcgacgac tactacgccg cacccaaccc agcgttcggc gccgtgctga
     5341 cctactacct gaaagacggg ctcgagacgc gcgccgaacg gcgccgccgg accgagaagg
     5401 agaccgaaca gcagggccgg ccctacgact acccggagga cgaggagtta cgggccgaaa
     5461 tgctcgagca cgacccggcg gtgctgctga cgattcgcga cgccgagggg cgggtcgtca
     5521 gacggctcga cggacccgtg acggcaggca tccaccggat cgcgtgggac ctgcgcttcc
     5581 cgccgtcgac accggtccga ctcgacgccg gcgaccccga cccgttcgat ccgccgccgg
     5641 tcgggccgat ggtgctgccc ggacgctaca cggtcgagct cgcgacggta gtcgacgggg
     5701 tcgtcgacac gctcggcgcg ccggagacga tcgtcgccac cccgctcggc ctggcgaccc
     5761 tcgcggccgg ggatcgcgcg ggcctgctcg agttccaacg ccggacggcc gacctgcagc
     5821 gtgccgtgct cggggcggtg agggtcgtcg acgagaccga gcgccggatc gaccacctgc
     5881 ggcgagcgat ccacgacacg ccgggtgccg accccgacct ggcggtcgag ctgcgcgcga
     5941 tcgagaatcg gctcgccgcc ttgacgatcg gcttgtcggg agacccgctg atccgtcgtt
     6001 acaacaaccc cgcgccccgc tcggtcgtcg accgcgtcga gcggatcgtg tcgagccaat
     6061 ggacctcgac cgcgccgccg acggggacca acctcgacgc ctaccggatc gccggcgaag
     6121 gcttcgcgcc gctactcgag gagctacgag cgatcgtcga gggcgatctc ccgcggatcg
     6181 aacggcggct cgaccaggtc ggcgcgccgt ggacgccggg gcgggtcccg cgatggtcga
     6241 tggacgacgc gcgaccctag aactactgac ggaatgccgc gggaatcggc ggcggcgaac
     6301 ccgccggtta cggagattac tatggtggcg cgcgggatcg tccgtactgg ctctctcaga
     6361 cgcaactcac cgcacgagag ggagcaacgc cgtgtcgacc caacaccgtc aaccccaagt
     6421 aaccccggcc cgggacggag agcgaggctc ggcgctgatc atggcgattt tcgtactcac
     6481 gctgctcggc ctggtcggcg tcgcgctgtt gcacctcacc tcgaccgaga tgacgatgag
     6541 caagcgcgcg ctcgacctca agcaggcgtt ctacctggcg gaggccggtg aggagcgcgc
     6601 acgaacgacg ctgtacctcg ccgacacgaa ccaggacttc tcggacgagc tggcgagcta
     6661 cgccggagcg aacggcgtgt tcgacctcga cgtcagcaac ttgaggctgg tcttcgatgg
     6721 cgcgggcgcc gtcaccggcg tgaccggcgt cggagacgat accccggtcc acgcgatcac
     6781 cgcgttcgcc agcggattct acgcggccta cgtgaccaac gacccggccg aggggatcac
     6841 gacgacgacc gacgccaacg acctggtcat gatcacggcc gtcggcgccg gccgggatcg
     6901 cgcggtcgag gtcgtgcagg cgatcgtcga gcgctggcag atcgtaccgc agctgccgcc
     6961 ggcgacgatc acgctgctcg gcgaccaacc gctgttcgac ggcggcacca gcgacccgca
     7021 cgactactcg ggcgaggact gcccgggcgg gatcccgaat ttcaacgtcc cgaccgtcgg
     7081 cacgatcggc tcggacgccg agctccagtc cgagctgggc atcgtcgagc cggacggccc
     7141 ggactacgat tccggcccgt tcagccagca agaaacgttc gtcgacctga ccaacctggc
     7201 cgatccgatg ctggtggcca acggcttcac cgggctcgac ccgatgtgga ccgactgcga
     7261 agcgctgcac gatctcgtcg aggagctgcg caagatcgcc gacgtcgagt gctcggccgg
     7321 gaccgcgtgc accataccgt cgcagctgac gtcgaacgtg atcttcgtca acggcgacta
     7381 cgagatcaac ccgcccggcg gggccggcac gctggtcgtg accggagagc tggaatacga
     7441 cggccgcgcc ggctgggaag gc
//
LOCUS       DASPGN010000026        18542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_473212, whole genome shotgun sequence.
ACCESSION   DASPGN010000026 DASPGN010000000
VERSION     DASPGN010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 18542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18542
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473212"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VD788_00765"
     CDS             complement(<1..697)
                     /locus_tag="VD788_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXV74821.1"
                     /translation="MKDRPLGRTTRIAGLLAPAVAWLIIVPLPTAAAASEVPPLVTDR
                     PDVTESSETVRRGSVQVEAGYGFARFDGETERLDVTAFPSTLVRVGLDPRIELRLEWD
                     GLISESREAGGQRTEETGSGNMALGVKIKLREEQGALPQLAMLVDAVLPTGSQTFRAD
                     RIDPAIRLAGSNRLTGRLGLSYNVGVGALTVEEAPADLDTEVLGRYSVALGIGLSERW
                     GAFVEAFGFLPAGD"
     gene            complement(848..1162)
                     /locus_tag="VD788_00770"
     CDS             complement(848..1162)
                     /locus_tag="VD788_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74822.1"
                     /translation="MKRASIVLALVLGLVAMPILAGDAAKGKTHDVTVEFVSYDAEGK
                     TVTFKTEAGEQKTAPVQDSVVKMLEKVHAGDKVVLTCQDSESGEHEAVTAVKPAKAEK
                     KA"
     gene            complement(1296..3536)
                     /locus_tag="VD788_00775"
     CDS             complement(1296..3536)
                     /locus_tag="VD788_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HXV74823.1"
                     /translation="MIRREDALDYHGGARPGKIEIRSTKPCLTPRELRLTYLPGASFP
                     TEEIVKNPAAAFRYTGRGNLVGVITNGTAVPGLGDVGPLAAKPMQEGVAVLFKRLADI
                     DVFDVELDARDPASFVDAVRMLEPTFGGINIKDIGAPDGLYVYDRLVEALNIPVFHEN
                     LYSTAVVAVAALLNAVDLTEKRIGEIRVVMCGAGTVGTGCGRLMLRLGVEADNLWVYD
                     VNGLLHPDRDDLHPYQRTFCRDVPARELAEGIRGADVFVGASAGDVLTLEMIRSMGRF
                     PLVLALATPEPEIDYEAARGSRQDVIVATSQGMCPNAVLDLLSFPYIFRGALDVRATR
                     ITEEMMLAAARSLAELARDDVPEEVERAYGNQRFTFGPEYLLPKPIDPRILVRESAAV
                     AQRAIEQGVAGSPLGREAHEESLVVRFGTGRETLRRLILRARQNRLRIVFSEGSNETI
                     LRACALLLDESIADPVLVGGEREIGEAVDRLGLDLSGITIADPARSPRVEHYVDEYFA
                     MRQRRGVMRHAAERRLRERDYFAAMMVRAGDADMMIAGVSTHYAETLRTIVEMIGTAA
                     GVGRISSHYLILLPSDVVVLADCAVNIDPDPEQLAEIALLAARMTRSLGIEPRVAMLS
                     FSNFGSVRHPAADKVRRATEIARRRAPELMVDGEIQLAAARDAALRSRYFPFSALEQD
                     ANVLVFPDLQSGNLALQALQQMGEAVAIGPVLMGTRRPVHVVQYGFSVEDVFNLATVG
                     VVEAGA"
     gene            complement(3533..7789)
                     /locus_tag="VD788_00780"
     CDS             complement(3533..7789)
                     /locus_tag="VD788_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXV74824.1"
                     /translation="MDATASRNDETFATTEPILERVAARCGRTLAQQVVAEIGSEGLN
                     LGLTAERFIGISIDGSLPFLVTPDEAPLLEACDGARRVSDSWLFVGHGGDPARSADPT
                     PDDALVKRFREFNLAHADVLEQIVDTAPLTRQRVMTILERMDRIVGDFIALFASHADE
                     CVILPEVHATIARRILAELDRDAAEAGRIAELSRLVQTFDDPASLGAVRTLHGLKRYL
                     HQRGLDLGMQLVRGRQSADRTVGLAFAAEGRVVRCIKRIRYADFNPAETAPADRSIPW
                     PVRLAIDAFGRQLLHGQEKFPHLEVFCYGNEVHYYLSFWNHPALLRVDFSPPLRGGMI
                     DLAYYGVSNYDEAVHPNPSLDAIRLLLRGLDFDVDVEYSKIHARLDKERTIDPARLRD
                     RAEAMLRLAPYLMDLDWTIGSLALDAEARLKVAEAWAESFRVWGVLPMYRLLTNDRRG
                     IVVAVEHDALGEHERVWSGAGPYRDRFHPPPTEGAFAEVRAALVRVGVEVPVATDDRR
                     PIGQLALERRLLGPLREASARGTSPEHPVERFAKMLAGGPAALADGVRAARLVPALSD
                     SVRFETIGTLNEYEVERARIELWGRALDLFALRDSIGIARLASFAWPSGTDFPAVDAA
                     ALLCRANYLTPGAELAAHVDDRETAELAERLRRPNPRPRSTAGSGRRLLRGRQASPGV
                     AAGRVVFATPGGAAERVDRAILVAPTVGPEDNNLIYRAAGVVTTGGGILSHAGLLAVQ
                     FGKPALIVAGRWVRDERDATALLYTVGQFDEDERMLAGCRIRIRRAPRERECRLEQGD
                     LVVLDAGEGTLEVLGQDPLALALHQEIGRLAEVARRASETTSDRQRLSLRGDRLRTER
                     QIARLLERLADADVARYAVRALLAGPFVPRLVCAALDNPRTGDDSRACLIEAIDEMVR
                     RDRVTRDELQRRVPSSTSLFEILSLRLDVLDARRSLEQGLATLAACAIDHPADGARPI
                     DELDGIVRSRLETLRAACATEIRAAAARGADFGLRHLLRQLERYDLVLRTPAERTARH
                     RPTRAALEANDDRVRRRLAGRRVLADHESGFESVQRIGWKAANLAEIGRLCGGALVPP
                     WFVVTQRAFDDALRSPVRERGAEADGAAPHERTLGASIAAALARREWSLEQRSRRIRQ
                     LWEHAELPHDVVAEVVAAYHRLIGEAPDAGVDADGGFVAIRSSSVEEDAETAARAGEF
                     ETYLFVRGDASVVTHLKRAWSGLWTERAIHNRAALGSSVPFPGGGIIVQRIVRARAAG
                     VLQTVNVGKGAPNELVVNAGLGLGEGVVGGLVLADQFTVAKDTGPPGGALRFHCVIAD
                     KAERIVFNRRLGAGTVRQPTAGGERLRPALDYAELHELVRAALDLERGYGYPLDIEFA
                     FEGSRLWILQARPVATYLSALRETLDRRPVSAKVSSRRPS"
     gene            complement(7906..8862)
                     /locus_tag="VD788_00785"
     CDS             complement(7906..8862)
                     /locus_tag="VD788_00785"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HXV74825.1"
                     /translation="MIDRASRLVLPALFCLATITFSSCGTGGPPAPATGAAEPGSDTH
                     PAWSPDGATVAYISNRGGVRAGKPINFEVYRVALDGSADRRLTSNQEFEADVAWSPDG
                     SEIVFKSYRDGNDEVYAMDADGGAQRNLTRSPAADGGASWSPDGSLLVFQSDRDGTGE
                     DRLHLMGPDGSNVRRLPLDPGPGHSPVWSPDGARIAFVSNRDGNGEIYVIDADGSNLR
                     RVTTDPRENGYPRWSPDGTTIAHTAGSFATDRWVVILTDADGGSSRQVVDDADSGNVS
                     WSPDGKRLLFARYSVYGDNGGDDSRLFVLDLDTGRETRVPNR"
     gene            complement(8859..9377)
                     /locus_tag="VD788_00790"
     CDS             complement(8859..9377)
                     /locus_tag="VD788_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV74826.1"
                     /translation="MVPEIDRAVRDYEAGRISRRELVGTLCAVVTAAVAGASAPSPAD
                     EPRSTFRSVGLNHVALRVTDIERSRRFYVEHLGLRVMRESESNCFLACGANHFVALFR
                     ASAAGLDHYCYTIDGYEAGAVMERLRAAGLAPERHDDRVYFDDPDGLTVQLSGEWDDY
                     PGGRPAGGGGGR"
     gene            complement(9421..10770)
                     /locus_tag="VD788_00795"
     CDS             complement(9421..10770)
                     /locus_tag="VD788_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXV74827.1"
                     /translation="MSMPPTTVTPLATYRNETPYPFCPGCGHGPILDRLNEALVSLQL
                     DPRRVVLVSDIGCSGLSDQYFVTSAFHGLHGRSITYASGIKLARPDLKVIVLMGDGGT
                     GIGGTHLLSAARRNIGVTVLVFNNFNFGMTGGQHSTTTPTGAITATTPGGNLERPLDL
                     CATVAVNGAGFVWRGTSFDRDLAERIAEAIRLEGFSLLDVWELCTAYYVPSNKASKRT
                     LAKTLAELGLEAGVLCRAERPEYTAARRAAGTDERSEPPLAPRPIEPRFASTLTRPFR
                     LIVAGSAGGKVRSAARLVAEAALGCGLRAALRDDYPITVKTGHSLSELVLSPEPIHYT
                     GIERADALVVLTPEGRAALGDRLARMEPSGRVFHFPTVTDLPTQAGLTPLDPGRLPAG
                     RAALFALAATVRALGILPLEALEEAARRGDARFARETLEAIAAARLAGLYKYSPSPP"
     gene            complement(10767..11897)
                     /locus_tag="VD788_00800"
     CDS             complement(10767..11897)
                     /locus_tag="VD788_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74828.1"
                     /translation="MRELIDGSTAIVRGALDAGCNFFAGYPITPASPILSAMLRELPR
                     RGGVAIQAEDEIASIGMCIGATLAGSRAMTATSGPGLSLYSENLGLAIMGEVPLVVVD
                     IQRLGPATGGATTVGQGDAQFARWGTAGGWPVIVLAPSSLVGCYTSTRRAFDLAERFR
                     CPVLLLGDKELGLTSGTIEPEELDAVPVRERAVAPGPAGAGLPAPRPYRFEPAASVPP
                     MVHYGGEAVVRFTGSTHDEHAFITKNPATVARLNEHLRAKIEDHAGEIELCERDLEPG
                     ARTLVVSWGVTAGAVREAVRSVRAAGRRVSSLVLESLWPFPDRALAEAVGDVERVVVA
                     ELNQGQLRREVERVVPRGVAVVGLHRVDGRLITPSEVEERVR"
     gene            complement(11978..13888)
                     /locus_tag="VD788_00805"
     CDS             complement(11978..13888)
                     /locus_tag="VD788_00805"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXV74829.1"
                     /translation="MRSIRETRRKRTGALTGLISVLALATAAGALAQDTSRPVLLGKH
                     WIAVTGKPQGATAGAMIFQQGGNAVDAAAAMLAATATMWDVLSWGGETQALIYNPHTR
                     EVLGINALGVAPTGATPEFFRERGMSYPPEFGPLAATTPGTPGGLMVMVAEYGKLRLA
                     EVLEPAIQLADGYPIEASQSANIERRREILEQWPDSRRVLLPHLDPADPSRRAAPAPG
                     EIFRQVELKATLEKLVEAEARALAAGKSRKDAIYAAYDRFYRGDIAEEIVRAAREHGG
                     LFTADDLADWEVRIEKPVSTDYRGIDVYKLTTWTQGPVMLQTLNILEGFDLRAMGYNS
                     TRYIHTLYQAMRLAYADRDFYYGDPYFPPAEPVAGLLSKEYAAKRRELISLDRVIDEI
                     KPGDPYPFQDETNPFEPLRRQWTPIPPAADAEGVEGFQQAGRISHDEGFFAGTTSIQA
                     ADAEGWVVSVTPSGGWIPAFVAGNTGVGLSQRMQSFVLDERLNPYNVVAPGKRPRVTL
                     TPTLALEDGRPLVAFSVQGGDSQDQNLLQFFLNIVEWEMNVQQAAEGRGNILSYQMQS
                     SFGAHRAEPGRLELTPLVPSYTREQLAKMGYDVELIERTYNPTTAIWFDREHGAMHGA
                     ASDYGDDYGIAW"
     gene            14267..18400
                     /locus_tag="VD788_00810"
     CDS             14267..18400
                     /locus_tag="VD788_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV74830.1"
                     /translation="MTTPGSLTARLNDGQFRELPLRHTDVRTEITSFVARTTVEQVFA
                     NPFDRPIEAVYLFPLGHEAAVDDFELECNGRIVRGEIHPRDEAHRIYEEARVQGHSAA
                     LLDQQRPNVFLQRVANLMPSSVIRVRIRTVELLRYRYGVYEYLFPTVVGPRYTPGAGP
                     DGEYASLEPERLGPGERSGHDIDIHVELHAGVPIADIVSPNHTVYVRQLSETTAGIDL
                     AEISRIPNKDFVLRWDVSSEHPATALLTHRSAGEGFFTLLLQPRGQVEIEEAAPKEIV
                     FVIDTSGSMDGTPIEMSKRFVTDALRTLGPNDRFNVVQFAGSSSVLFSDSVAGDSKSI
                     GRAIEYIEQLRGHGGTELLGGLRKAVDTKPAAGQYRQIVFLTDGLIGNDDRVIGEVSK
                     LPEQYRIFTVGLGESVNHHLLTRMAEVGHGASARIVAEADGWDALKEFREWVTRPYLT
                     DIEIDWGGLPLGDLVPRQQRDLYSGQTLYLIGRYYGHARGEIVLRGYEGGSYWEQTVV
                     VDLPEVDEANGVLASIWGRRRIGELMLAMQENGEEKTRWLVTDLAMRYRLMSPYTSFV
                     AVDHSTVVNEDAGWVSVEQALPSIEVTRVETISGAETRERVVVTATSAVVDLSSAAMT
                     TRFTDEFMRDLPVPGRFYQNVLTLAPGVQDAGGDGSPNVHGSRARSFRTVVDGIANVD
                     LLTGQMMSQIQPNSIEEMEVIAAGAGVEFGRAQGGFTRILQTQGGNQFEGLVELGYRV
                     PVGDGAGWNGSSRPGWQAGIQLSGPIVKNRLWYRLSHDGLDRDQPIDDTRSVTAVPTR
                     ASAHSDRITWQVSPRNKLTVDLAKERVSLDNVGLSSWIGPDSTRSVRSGGPRYVLSWI
                     VPYSPKILVQASVAYQSSEFEVVPSRPGAANDCIASLAAEPDRTACLDLTAAVVSGPA
                     HHEWEQDRSRLSVSGQSTIYAGRAIGATHQLELGFEVHDDRFDRTIVRGPTLIRGSDT
                     DGSGRLTGRIADDPIGRDGARGTLWGVWAQDQLKPSQNLTLTLGLRADRYELEAVGYA
                     PFDPNLPAEASARRAQNLLIAATNVSPALSVAWDPWMDGKSKFFLSAKRVHDELSPAV
                     LLIEQEPLTRDVDLTRVTVDPERATGPQNVQIVDRDLEAPYLDEWTVGFERQIFTETS
                     IRATLIAREYRDRLGFVDLNRTLLSPYWGEIVSIGNLHSARYRAFRLEWTRRQYRNWE
                     LQADYTYSVLEGDAQDLDLLVGDERSRFEVAPGYQAGDQRHFFRADATWITPWGIRLG
                     GALRVGSGLPFSRVESSWRPVGETAVPVPRFVHPSGRRNDLRNPPTWTVDFRMARDFS
                     AGRAVDIRVALEVFHLFGNDPYLVYNDARGSGQRIDGVDELTRFSRREVQLGVELRF"
BASE COUNT         2864 a         6535 c         6306 g         2837 t
ORIGIN      
        1 cgtcgcctgc cgggaggaaa ccgaacgcct cgacgaacgc cccccaacgc tcggacagac
       61 cgataccgag ggcgacgctg tatcggccga gcacctcggt atcgagatcc gccggggcct
      121 cttcgaccgt caacgcgccg acgccgacgt tgtagctcag gccgagccgc ccggtcaggc
      181 ggttcgagcc ggccagccgg atcgccgggt cgatgcggtc ggcgcgaaag gtctggctcc
      241 ccgtcggcag gaccgcgtcg acgagcatgg caagctgcgg caaggcgccc tgctcctcgc
      301 gcagcttgat cttgacgccg agcgccatgt tgcccgagcc ggtctcctcg gtgcgctggc
      361 ctcccgcctc acggctctcg ctgatcagac cgtcccactc caagcgcagc tcgattcgcg
      421 gatcgagacc gacgcgcacg agcgtgctcg ggaacgcggt gacgtcgagc cgctccgtct
      481 cgccgtcgaa tcgggcgaag ccgtagcctg cctcgacctg gacggagccc cggcgcacgg
      541 tttcgctcga ctcggtcacg tcgggacgat cggtcacgag cggcggcacc tcggacgccg
      601 ccgccgccgt cggaagcgga acgataatca gccaagcgac ggccggcgcc agcagaccgg
      661 cgattctcgt cgtgcgaccg aggggtcgat ccttcatttt gtgtcgccga atcggaattt
      721 ttgatctatc aaaaccgatc gatcggggac actaccatgc ggctcgacgg ccggtcaacg
      781 tctcgcgaaa caaaaaaggc gcgcggggag accccgcacg ccctcatcga tcggtgctcg
      841 gcccggttca ggctttcttt tcggccttgg ccggcttcac cgccgtcacc gcttcatgct
      901 cgccggactc gctgtcctgg caggtgagca cgaccttgtc accggcatgc accttctcca
      961 gcatcttgac gacgctgtcc tggaccggcg cggtcttctg ttcgccggcc tcggtcttga
     1021 aggtcacggt cttcccttcc gcgtcgtacg acacgaactc gacggtcacg tcgtgggtct
     1081 tgcccttcgc cgcgtcaccg gccagaatcg gcatggccac gagacccaac accaacgcca
     1141 atacgatcga agctctcttc atcgaagtcc tcctcgcggg aaccgcgctg cgtcccgctg
     1201 gttcatccgc caggctagcg gatccccgcc gcgcggtcca cccggcgcgg gtcgccggtg
     1261 aaaaacagcg gaattcctgg caaaaaacga ctccctcacg ctcctgcctc gaccacgccg
     1321 acggtcgcca agttgaacac gtcctcgacc gagaagccgt actggacgac gtggaccggc
     1381 cggcgggtgc ccatcaacac cgggccgatc gccaccgcct cgcccatctg ctgcagcgcc
     1441 tggagcgcga ggttgccgga ctgcaggtcg ggaaagacca gcacgttggc gtcctgctcg
     1501 agcgccgaga acgggaagta gcggctgcgc agcgcggcgt cgcgtgcggc ggccagttgg
     1561 atctcgccgt cgaccatcag ctcgggcgca cgtcggcggg cgatctccgt cgcccggcgg
     1621 accttgtcgg ccgccgggtg gcggacgctg ccgaagttcg agaacgacag catcgccacg
     1681 cgcggctcga tgcccaaaga gcgggtcatc cgcgcggcga gcagcgcgat ctcggccagt
     1741 tgctcggggt ccgggtcgat gttcaccgcg cagtcggcca gcacgacgac gtcgctcggc
     1801 agcaggatca ggtagtggct cgagattctc ccgacccccg ccgcggtacc aatcatctcg
     1861 acgatcgtgc gcagggtctc ggcgtagtgc gtcgaaacgc ccgcaatcat catgtccgcg
     1921 tcgcccgcgc gcaccatcat cgccgcgaag tagtctcgct cgcgcaacct gcgctcggcg
     1981 gcgtgacgca tcacccctcg gcgttgacgc atcgcgaagt actcgtcgac gtaatgctcg
     2041 acgcgcgggc tgcgcgccgg gtcggcgatc gtgatgccgc tcaggtcgag accgagacga
     2101 tcgaccgcct cgccgatctc ccgctcgccg ccgacgagga cggggtcggc gatcgactcg
     2161 tccaacagca gcgcacaggc gcgaaggatc gtttcgttgc tgccctcgga gaagacgatt
     2221 ctaagtcggt tctggcgcgc gcgcaggatc aggcggcgca gcgtttcgcg tccggtgccg
     2281 aatcggacga ccagactctc ctcgtgtgcc tcgcggccga gcgggctgcc ggcgacgccc
     2341 tgctcgatcg cccgctgggc caccgccgcc gactcgcgca cgaggatgcg tgggtcgatc
     2401 ggtttcggca gcaggtactc gggtccgaag gtgaagcgct ggttgccgta ggcgcgctcg
     2461 acctcctccg gcacgtcgtc ccgcgccagc tcggccagcg accgcgcagc ggcgagcatc
     2521 atctcttcgg tgatgcgcgt ggcacgcacg tcgagtgcgc cgcggaagat gtaggggaag
     2581 ctcaacaggt cgagcaccgc gttcggacac ataccctggc tcgtcgccac gatcacgtcc
     2641 tgccggctgc cccgggccgc ttcgtagtcg atctcgggtt cgggcgtcgc cagggccagc
     2701 accaacggaa agcggcccat cgagcggatc atctcgagcg tcagcacgtc gccggccgag
     2761 gccccgacga agacatcggc gccgcgaatc ccctcggcca gctcgcgtgc gggaacgtcg
     2821 cggcagaacg tgcgctggta cgggtgcagg tcgtcgcggt cgggatgcaa cagaccgttg
     2881 acgtcgtaga cccacaggtt gtcggcctcg acgccgagcc gcagcatcaa tcggccgcag
     2941 cccgtgccga ccgtcccggc gccgcacatc acgacgcgga tctcgccgat tcgtttctcg
     3001 gtcaggtcga ccgcgttgag cagcgccgcg acggcgacga cggccgtgct gtacaggttc
     3061 tcgtggaaca ccgggatgtt gagcgcctcg acgagacgat cgtagacgta cagcccgtcc
     3121 ggtgcgccga tgtccttgat gttgatgccg ccgaaggtcg gctcgagcat gcgcaccgcg
     3181 tcgacgaagc tcgccggatc ccgcgcgtcg agctcgacgt cgaagacgtc gatgtcggcc
     3241 agacgcttga acaggaccgc cacgccttcc tgcatcggct tggccgccag cggacccacg
     3301 tcgcccaagc ccggcacggc agtcccgttg gtgatcacgc cgaccaggtt gccccgcccc
     3361 gtgtaacgaa aggccgccgc cgggttcttg acgatctcct cggtcgggaa cgacgccccc
     3421 ggcaggtagg tcaatcgcag ctcgcgcggc gtcagacagg gcttggtcga gcggatctcg
     3481 atcttccccg gacgggcgcc cccgtggtag tcgagcgcat cttcacgccg aatcatgacg
     3541 gtctccttga gctgaccttc gcactgaccg gccggcggtc gagcgtctcg cgcagcgccg
     3601 acaggtaggt cgcgacgggt cgtgcctgca ggatccacag ccgcgatccc tcgaatgcga
     3661 actcgatgtc gagcgggtag ccgtagccgc gctcgagatc gagcgccgcc cgcaccagct
     3721 cgtgcagctc ggcgtagtcg agcgcgggcc gcaaccgttc gccgccggcc gtcggctggc
     3781 gtaccgtgcc ggcgccgagc cgccggttga acacgattct ctcggccttg tccgcgatca
     3841 cgcaatggaa gcgcaacgcc ccgcccggcg ggccggtgtc cttggccacc gtgaactgat
     3901 cggccaacac caggccgccg acgaccccct cgccgaggcc cagccccgcg ttgaccacca
     3961 gttcgttcgg cgcgcccttg ccgacgttga ccgtctgcag cacgccggcc gcgcgggctc
     4021 gcacgatgcg ctgcacgatg atgccccctc ccggaaacgg cacggacgag ccgagcgcgg
     4081 cgcggttgtg aatcgcccgt tcggtccaca gcccgctcca cgcccgcttg aggtgagtca
     4141 ccaccgaggc gtcgccgcgg acgaacaggt acgtctcgaa ctcgccggcg cgggccgccg
     4201 tctcggcgtc ttcctcgacg ctcgacgatc ggatcgcgac gaaaccgccg tcggcgtcga
     4261 cgccggcatc cggcgcctct ccgatcagcc ggtggtaggc cgccacgacc tccgcaacca
     4321 cgtcgtgcgg aagctcggcg tgctcccaca gctggcggat ccgccgtgat ctctgctcga
     4381 ggctccactc gcgtcgagcg agcgcggccg cgatcgacgc gccgagcgtg cgttcgtgag
     4441 gcgccgcgcc gtccgcctcc gcgccccgtt cccgcaccgg cgaacgcagc gcgtcgtcga
     4501 aggcccgctg ggtcacgacg aaccacggcg ggaccagcgc gcccccgcac aaccggccga
     4561 tctcggcgag gttggccgcc ttccagccga tccgctgcac cgactcgaag ccgctctcgt
     4621 ggtcggcgag cacccggcga ccggccagcc gccggcgcac gcggtcgtcg ttcgcctcga
     4681 gcgccgcgcg cgtcggtcga tggcgcgcgg tccgttcggc gggcgtgcgc aggacgaggt
     4741 cgtagcgctc gagctgtcgc agcaggtgcc gcagtccgaa gtccgctccg cgagcggccg
     4801 cagctcggat ctcggtcgcg cacgccgccc gcagcgtctc gagcctcgac cggacgatcc
     4861 cgtcgagttc gtcgatcggt cgggcaccgt cggccgggtg gtcgatcgcg caggccgcga
     4921 gcgtcgccaa tccttgctcg agcgatcggc gcgcgtcgag cacgtcgaga cgcagcgaca
     4981 ggatctcgaa cagcgaggtc gacgacggga cgcgccgttg cagctcgtct cgcgtcacgc
     5041 ggtctcggcg caccatctcg tcgatcgcct cgatcagaca ggcgcggctg tcgtcaccgg
     5101 tccgcgggtt gtcgagcgcc gcgcagacga gccgcggaac gaagggcccg gcaagcagcg
     5161 cgcgaacggc gtaacgcgcc acgtcggcgt ccgcgagccg ctcgagcagt ctcgcgatct
     5221 gacgctcggt ccgcagccga tcgccgcgga ggctcaagcg ctgccggtcc gacgtcgtct
     5281 cgctcgcacg ccgggcgacc tcggccaatc gtccgatctc ctggtgcagc gccagcgcca
     5341 gcgggtcctg cccgagcacc tcgagcgtgc cttcgccggc gtcgagcacc acgagatccc
     5401 cttgttccag gcggcactcg cgctcccgcg gcgcccgtcg aatgcggatc cggcagccgg
     5461 cgagcatccg ctcgtcctcg tcgaactgac cgacggtgta gagcagtgcc gtcgcgtcgc
     5521 gttcgtcgcg gacccagcgg ccggcgacga tcagcgccgg tttgccgaac tgcaccgcca
     5581 gcaagccggc gtggctcagg atcccgcccc ccgtcgtgac cacccccgcg gcgcggtaga
     5641 tcagattgtt gtcttcggga ccgaccgtcg gggcgacgag gatcgcgcga tcgacccgct
     5701 cggccgcgcc gcccggggtg gcgaagacga cccgccccgc ggcgacgccc ggcgaggcct
     5761 gacgaccgcg cagcaggcgc cgccccgacc ccgcggtcga tcgcgggcgc gggttcggcc
     5821 gcctcagccg ctcggcgagc tcggcggtct cgcggtcgtc gacgtgggcc gcgagctcgg
     5881 ctcccggcgt gaggtagttg gcgcggcaaa gcagcgccgc ggcgtcgacc gccgggaagt
     5941 ccgtgccgga cggccacgcg aaggaggcca accgggcgat gccgatcgaa tctcgcagcg
     6001 cgaacaggtc gagcgctcgt ccccacaact cgattcgtgc gcgctcgacc tcgtattcgt
     6061 tgagcgtgcc gatcgtctcg aagcgcacgc tgtccgacag agccgggacc aaccgcgcgg
     6121 cccggacgcc gtcggccagc gccgcgggtc cgccggccag catcttcgcg aaccgctcga
     6181 ccggatgctc gggcgaggtc ccgcgtgccg acgcctcgcg cagcgggccg agcaggcgac
     6241 gctcgagcgc gagttgcccg atcggtcgcc ggtcgtcggt cgccaccggc acctcgaccc
     6301 cgactcgcac gagcgccgcc cggacctcgg cgaaagcgcc ctccgtcggc ggcgggtgga
     6361 atcgatcgcg gtacgggccc gcgcccgacc agacccgctc gtgctcgccg agcgcgtcgt
     6421 gctcgaccgc gacgacgatc ccgcgacgat cgttcgtcaa cagacggtac atcggcagca
     6481 cgccccacac acggaacgac tcggcccatg cctcggcgac cttgaggcgc gcctcggcat
     6541 cgagggccaa cgagccgatc gtccagtcca ggtccatcag gtacggcgcg agtcgcagca
     6601 tcgcttcggc ccgatcgcgc agccgcgccg ggtcgatcgt ccgctccttg tccagccgcg
     6661 cgtggatctt cgagtattcg acgtcgacgt cgaagtccag accgcgcaac aacagccgga
     6721 tcgcatcgag cgacgggttg ggatggacgg cctcgtcgta gttgctgacc ccgtagtagg
     6781 cgagatcgat catccctccc cgcagcggcg gagagaagtc gacgcgcagc agggcgggat
     6841 gattccagaa ggacaggtag tagtggacct cgttgccgta acagaagacc tcgaggtgcg
     6901 ggaacttctc ctgcccgtgg agcagctgac gtccgaaggc gtcgatcgcc aagcgcaccg
     6961 gccagggtat cgatctgtcg gccggagcgg tctccgccgg gttgaagtcc gcgtagcgga
     7021 ttcgcttgat gcagcgcacc acccgccctt cggcggcgaa cgccaggccg accgtgcgat
     7081 ccgccgattg ccggccgcgc acgagctgca tgccgaggtc gagcccgcgc tggtgcaggt
     7141 agcgcttgag accgtgcagc gtgcgaaccg cgccgagcga cgcggggtcg tcgaaggtct
     7201 ggaccagccg cgacagctcc gcgatccggc ccgcctccgc cgcgtcgcgg tcgagctcgg
     7261 cgaggatgcg ccgtgcgatc gtcgcgtgga cctcgggcag gatcacgcac tcgtcggcgt
     7321 gcgaggcgaa cagcgcgatg aaatcgccga cgatgcggtc catccgctcg aggatcgtca
     7381 tcacgcgctg tcgcgtcagc ggcgccgtgt cgacgatctg ctcgagcacg tcggcatgag
     7441 ccaggttgaa ctcgcggaac cgcttcacca gcgcgtcgtc gggcgtcggg tcggcgcttc
     7501 gggcgggatc gccgccgtgg ccgacgaaca gccaagagtc ggagacgcgg cgcgctccgt
     7561 cgcaggcctc gagcaacgga gcctcgtccg gagtgacgag gaacggaagc gacccgtcga
     7621 tcgagatccc gatgaaccgc tcggcggtca agccgaggtt cagcccttcg gatccgatct
     7681 cggcgacgac ctgctgtgcc agcgtccgac cgcaccgtgc cgcgacgcgc tcgaggatcg
     7741 gctcggtggt tgcgaacgtc tcgtcgttac ggcttgcggt ggcatccacg tcggtctacc
     7801 gcacgagctg cctccgctcc caacctacaa ccgcggaccg acggtgtcga taccgggggc
     7861 cgcgacaatg tagagtctca gggtcgccga tcccccgggg cgacgtcatc gattggggac
     7921 gcgcgtctcc cgcccggtat cgaggtcgag gacgaacaga cggctgtcgt cgccgccgtt
     7981 gtcaccgtac acgctgtaac gcgcgaacag cagtcgcttg ccgtcgggcg accacgagac
     8041 gttgccgctg tccgcatcgt cgacgacctg gcgcgagctg ccaccgtcgg cgtcggtgag
     8101 gatcacgacc caccggtcgg tggcgaagct cccggcggta tgagcgatcg tcgtaccgtc
     8161 cggcgaccac cgcgggtagc cgttctcgcg cggatcggtc gtgacgcgac gcaggttcga
     8221 gccgtcggcg tcgatcacgt agatctcgcc gttgccgtcg cgattcgaga cgaacgcgat
     8281 ccgcgcgccg tcgggcgacc agacgggcga gtgcccgggg ccggggtcga gcggcagccg
     8341 acgcacgttc gagccgtccg gccccatcag atgaagccga tcctctcccg tcccgtcgcg
     8401 gtccgactgg aagaccagca gcgagccgtc cggcgaccag ctcgcgccgc cgtccgcggc
     8461 cggcgagcgg gtcaggttgc gctgggcacc gccgtcggca tccatcgcgt agacctcgtc
     8521 gttcccgtcg cggtacgact tgaacacgat ctcgctgccg tccggcgacc aggccacgtc
     8581 ggcctcgaac tcctggttcg aagtgagacg ccggtccgcc gacccatcga gcgcgacgcg
     8641 gtagacctcg aagttgatcg gcttgcccgc tcgcacgccc ccgcggttgg agatgtacgc
     8701 gacggtcgcg ccgtccggcg accaggccgg atgggtgtcg ctgcccggct cggccgcgcc
     8761 ggtcgccggg gccggcgggc ccccggtacc acagctcgag aacgtgatcg tggcgaggca
     8821 gaagagagcg ggcaggacga ggcgcgaggc tcggtcgatc accggccgcc ccctccgccc
     8881 gcggggcgcc cgcccgggta gtcgtcccat tcgccggaga gctgcacggt caggccgtcc
     8941 ggatcgtcga agtagacgcg atcgtcgtgg cgttcgggtg cgagcccggc cgcacgcagg
     9001 cgttccatca ccgctcccgc ctcgtacccg tcgatcgtgt agcaatagtg atcgagtccc
     9061 gccgcgctcg cgcggaacag ggcgacgaag tggttcgcac cgcaggccag gaagcagttg
     9121 ctctcgctct cgcgcatcac gcgcagcccg aggtgctcga cgtagaatcg gcgagagcgc
     9181 tcgatgtcgg tgacgcgcag ggcgacgtga ttcaatccga ccgagcggaa cgtgctccgc
     9241 ggctcgtcgg ccgggctcgg cgcgctcgca ccggcgaccg cggcggtcac gaccgcgcac
     9301 agcgttccga ccagctctcg gcgggagatc cggccggctt cgtagtctcg tacggctcga
     9361 tcgatctcag gtaccatgat cacctcgacc gacctctcgc cgccgatgct aacgctcgaa
     9421 tcacggcggc gagggtgaat acttgtaaag ccccgcgagg cgcgccgcgg cgatcgcctc
     9481 cagcgtctcg cgtgcgaacc gcgcgtcccc gcggcgcgcg gcttcctcga gcgcttcgag
     9541 cggcagaatg ccgagcgccc gcaccgtcgc agccagggcg aacagcgccg cgcggccggc
     9601 cggcaggcgg cccgggtcga gcggcgtcag gccggcctgc gtcggcaggt cggtcacggt
     9661 cgggaagtgg aacacgcgcc ccgagggttc catccgggcg agccggtcgc cgagcgcggc
     9721 gcgtccctcg ggcgtcagta ccaccagcgc gtccgcacgt tcgatcccgg tatagtggat
     9781 cggctcgggc gagaggacca gctcggacag gctgtggccg gtcttcaccg tgatcgggta
     9841 gtcgtcgcgc agggcggccc gcagcccgca gccgagcgcc gcttccgcga cgaggcgcgc
     9901 ggccgagcgc accttgccgc cggccgagcc ggcgacgatc agccggaacg gtcgcgtcag
     9961 cgtcgaggcg aagcgcggct cgatcgggcg gggcgcgagc ggcggctcgc ttcgctcgtc
    10021 cgtgccggcg gcgcgacggg ccgcggtgta ctccgggcgc tcggcccgac agagcacgcc
    10081 cgcttcgagt cccagctccg cgagcgtctt ggccagcgtg cgcttcgacg ccttgttcga
    10141 cggcacgtag tacgcggtgc acagctccca gacgtcgagc agcgagaagc cctcgagacg
    10201 gatcgcctcg gcgatacgct cggcgaggtc acggtcgaag ctggtgccgc gccagacgaa
    10261 ccccgctccg ttgaccgcga ccgtggcgca caggtcgagc ggccgctcga ggttgccgcc
    10321 gggcgtggtc gccgtgatgg cgccggtcgg cgtggtggtc gagtgctggc cgcccgtcat
    10381 gccgaagttg aagttgttga acaccagcac cgtcacgccg atgttgcgac gcgcggcgct
    10441 cagcaggtgc gtcccgccga ttcccgtgcc cccgtctccc atcagcacga tcactttcag
    10501 gtcggggcgc gccagcttga tcccggaggc gtaggtgatg ctgcgtccgt gaagaccgtg
    10561 gaacgcgctg gtcacgaagt actggtccga cagacccgag cagccgatgt cgctgaccag
    10621 cacgacacgc cgggggtcga gctgcagcga gacgagggcc tcgttcaatc ggtccagaat
    10681 cggcccgtgc ccacagccgg ggcagaacgg gtagggcgtt tcgttgcgat aggtcgcgag
    10741 cggcgtgacc gtggtgggcg gcatgctcat cgcacgcgct cctcgacctc ggacggtgtg
    10801 atcaagcgac cgtcgacgcg gtgcaggccg accaccgcga cgccccgcgg cacgacccgc
    10861 tcgacctcgc gccgcagctg tccctggttc agctcggcga cgacgacgcg ctcgacgtcg
    10921 ccgaccgcct cggcgagcgc tcgatcgggg aacggccaca ggctctcgag cacgagcgac
    10981 gagactctac ggccggctgc tcgcacgctc cgtaccgcct cgcgcaccgc gccggccgtg
    11041 acgccccagc tcacgaccag tgtccgtgcg ccgggctcga ggtcgcgctc gcacagctcg
    11101 atctcgcccg cgtggtcctc gatcttggcc cgcaggtgct cgttgagccg cgcgaccgtg
    11161 gcggggttct tggtgatgaa cgcatgctcg tcgtgggtcg agccggtgaa gcgcacgacg
    11221 gcctcaccgc cgtagtgcac catcggcggc acggaggcgg cgggctcgaa acggtagggc
    11281 cgtggcgcgg gcaggcccgc cccggcaggg cccggcgcca cggcgcgctc gcgcacgggc
    11341 acggcatcca actcctcggg ctcgatcgtc ccgctcgtca ggcccagctc cttgtcaccg
    11401 agcagcagca cgggacagcg gaagcgctcg gcgaggtcga aggcccggcg cgtcgacgtg
    11461 tagcaaccga cgagcgacga gggcgccagc acgatcaccg gccagccgcc ggcggtgccc
    11521 cagcgcgcga actgcgcgtc gccctggccg accgtcgtcg cgccgccggt cgccgggccc
    11581 aggcgctgga tgtcgacgac caccagcggc acctcgccca tgatcgcgag acccaggttc
    11641 tcgctgtaca gactcaagcc cggaccgctc gtcgccgtca tcgcgcgcga gccggccagc
    11701 gtcgcgccga tgcacatgcc gatcgacgcg atctcgtcct cggcctggat cgcgacaccg
    11761 cctcgccggg gcagctcgcg cagcatcgcg ctcaggatcg gcgacgccgg cgtgatcgga
    11821 tagccggcaa agaagttgca acccgcgtcg agcgccccgc ggacgatcgc ggtcgagcca
    11881 tcgatcaact cgcgcacaac ggccctcgct cggccggcgc ggtgccccgc accgccctgg
    11941 gacccacgat actactcgcg acgacggccg ccagcgatca ccaggcgatc ccgtagtcgt
    12001 cgccgtagtc gctggccgcc ccgtgcatcg cgccgtgctc ccgatcgaac cagatcgcgg
    12061 tggtcgggtt gtaggtgcgc tcgatcagct cgacgtcgta gcccatcttc gccaactgct
    12121 cgcgggtata gctcggaacc agcggtgtga gctcgagtcg ccccggctcg gcccggtggg
    12181 cgccgaagga actctgcatc tggtagctga ggatgttgcc gcggccttcg gcggcctgct
    12241 gcacgttcat ctcccactcg acgatgttga ggaagaactg caacaggttc tggtcctgcg
    12301 agtcgccacc ctggaccgag aacgccacca gcggccggcc gtcctccagc gcgagggtcg
    12361 gcgtcaacgt gacgcgtggt cgcttgcccg gcgcgacgac gttgtacgga ttcaaccgtt
    12421 cgtccagcac gaagctctgc atacgctggc tcagacccac ccccgtgttg ccggcgacga
    12481 aggccgggat ccaaccaccg gacggggtga ccgaaaccac ccagccttcg gcatcggcgg
    12541 cctggatcga ggtcgtgccg gcgaagaagc cctcgtcgtg gctgatccgt ccggcctgtt
    12601 ggaagccctc cacgccctcg gcgtcggccg cgggtggaat cggcgtccat tgccggcgca
    12661 gcggctcgaa cgggttggtc tcgtcctgga acgggtaggg atcgcccggc ttgatctcgt
    12721 cgatcacgcg atccagcgaa atcagctcgc ggcgctttgc ggcgtactcc ttcgacagca
    12781 ggccggcgac cggctcggcc ggcgggaagt acgggtcgcc gtagtagaaa tcgcgatcgg
    12841 cgtaggccag cctcatcgcc tggtacagcg tgtggatata gcgcgtcgag ttgtagccca
    12901 tcgcccgcag atcgaagcct tccaggatgt tcaacgtctg cagcatgacc ggtccctgcg
    12961 tccaggtggt cagcttgtag acgtcgatgc cgcgatagtc ggtgctgacc ggcttctcga
    13021 tccgcacttc ccagtcggcc aggtcgtcgg ccgtgaacag cccgccgtgt tcgcgcgcgg
    13081 cccgcacgat ctcctcggcg atgtcgccgc gatagaagcg gtcgtaggcg gcatagatcg
    13141 cgtccttgcg gctcttgccc gcggccagcg ctcgagcctc ggcctcgacc agtttctcca
    13201 acgtggcctt cagctcgacc tggcggaaga tctcgccggg cgccggcgcg gcccgccgcg
    13261 acgggtcggc gggatcgaga tgcggcagca gcacccggcg tgagtcgggc cattgctcga
    13321 ggatttcgcg ccggcgttcg atgttggccg actgcgaggc ttcgatcggg tagccatcgg
    13381 ccaactggat cgcgggttcc agcacctcgg ccaggcgaag cttgccgtac tcggcgacca
    13441 tcaccatcag cccgcccggc gtccccggcg tggtcgcggc gagcgggccg aactcgggtg
    13501 ggtacgacat accccgctcg cggaagaact ccggggtggc gccggtcggt gccacgccca
    13561 gcgcgttgat gcccagcacc tcccgcgtat gggggttgta gatcagcgcc tgcgtctcgc
    13621 caccccagct cagcacgtcc cacatcgtcg cggtggcggc cagcatcgcc gcggcggcat
    13681 cgaccgcgtt gccgccctgc tggaagatca tcgcgccggc ggtcgcaccc tgcggtttcc
    13741 cggtcactgc gatccagtgt ttgcccagca gcacgggccg actggtgtcc tgggccaggg
    13801 caccggccgc ggtcgccagc gccaggacgg agatcagacc cgtcagggcg ccggttcgct
    13861 tccttcgcgt ctctcgaatc gagcgcatgg cgacctttcg tgagaacggc acgaatcgat
    13921 ccgacattct attcccccac cgaggttcgg cgtcgtcgcc cggctccatc gacacggctc
    13981 cgagagctta catccaccag ctcgctttcg tagcgtggtc gttcggtccg cggcgaatcc
    14041 aggccgcaca tcgttcacac gacggatctc gcgcgtgcgg ttcgaactca gcttcaagcg
    14101 ctccgcgagc ccgacgtcgt tccgtgatcg caccacaaga gagatacaca gctcgcgatc
    14161 atcaggaggc aacaccgtgc atcgcacgac ccttcgcttg ctcttctcgt cgctcgtaat
    14221 ggccgcgacg gccaacggcg ccgacgcatc tcttggcgag gagtcgatga cgactcccgg
    14281 atcgctcacg gcgcgtctga acgatggtca gtttcgcgag ctccccctcc ggcacaccga
    14341 cgtccggacc gagatcacgt cgttcgtcgc gcgcacgacg gtcgagcagg tgttcgccaa
    14401 cccgttcgat cgaccgatcg aggcggtcta tctcttcccc ctcggtcacg aggccgcggt
    14461 cgacgacttc gagctcgagt gcaatggcag gatcgtgcgc ggggagattc atccgcggga
    14521 cgaggcacac cgtatctacg aggaggcgcg ggtccagggt cacagcgcag cgttgctcga
    14581 ccagcagcga ccgaacgttt tccttcaacg ggtcgccaac ctgatgccct cgagtgtgat
    14641 ccgtgttcgt atccgcacgg tcgagctgct gcgctaccgg tacggcgtct acgagtacct
    14701 gtttccgaca gtcgtcgggc cgcgttacac gccgggagcc ggtccggacg gagagtacgc
    14761 ttcgctcgag ccggagaggt tggggccggg agagcgttcg ggtcacgaca tcgacatcca
    14821 cgtggaactg cacgccggcg ttccgatcgc cgacatcgtc agcccgaacc acacggtcta
    14881 cgtccgtcag ctctcggaga caaccgccgg gatcgacctg gccgagatct cgcggatccc
    14941 caacaaagac ttcgtcctac gctgggacgt gtcgtcggag cacccggcga cggccctact
    15001 cacccatcga agtgccgggg aaggcttctt cacgctgctg ctccaaccca ggggtcaggt
    15061 cgagatcgag gaggccgcgc cgaaagagat cgtgttcgtg atcgatacgt ccgggagcat
    15121 ggacggcacg ccgatcgaga tgtcgaagcg gttcgtgacc gacgcgctgc ggaccttggg
    15181 gccgaacgat cgattcaacg tggtgcagtt cgccggcagc tcgtcggtac tcttcagcga
    15241 ttcggtagcg ggcgactcga aatcgatcgg gcgggcgatc gagtacatcg agcagctgag
    15301 aggccacggc ggaaccgagc tcctcggagg gctcaggaaa gcggtggata ccaagcccgc
    15361 cgccggacag taccggcaga tcgtgttcct gaccgacggt ctcatcggga acgacgatcg
    15421 ggtgatcggc gaagtctcga agctccccga gcagtaccgg atcttcaccg tcggactcgg
    15481 cgaatcggtc aatcaccacc tactgacccg catggcggag gtcggtcacg gagcgtccgc
    15541 ccggatcgtg gccgaggccg acgggtggga cgcactgaag gagtttcgcg aatgggtcac
    15601 gcgaccgtat ctcaccgaca tcgagatcga ctggggcggt ctgccgctgg gcgatctcgt
    15661 tccccgccag cagcgcgacc tctattcggg ccagaccctg tacctgatcg gccggtacta
    15721 cggccacgcc cgcggggaga tcgtgctccg cgggtacgaa ggcgggagct actgggagca
    15781 gacggtcgtg gtcgaccttc ccgaggtcga tgaagcgaac ggggtgctgg cctcgatctg
    15841 gggtcgccgt cggatcggcg agctgatgct cgcgatgcag gagaacggcg aggagaaaac
    15901 ccgctggctc gtgaccgatc tggcgatgcg ctaccggttg atgagcccct acacctcgtt
    15961 cgtcgcggtc gatcactcga ccgtggtgaa cgaggacgcc gggtgggtca gcgtggagca
    16021 ggcccttcct tcgatcgaag tgacgcgcgt cgagacgatc agtggagcgg agactcgaga
    16081 gcgggtggtc gtgacggcga cctcggcggt cgtcgacctg agctcggcgg caatgaccac
    16141 gagattcacc gacgagttca tgcgggacct tccggtaccc ggccggttct atcagaacgt
    16201 gttgaccttg gctccgggcg tgcaggacgc gggcggggac ggcagcccca acgtccacgg
    16261 atcacgcgct cgcagcttcc ggacggtcgt cgacggaatc gcgaacgtcg acctgctgac
    16321 cggtcagatg atgtcccaga tccagccgaa ctcgatcgag gagatggaag tgatcgcggc
    16381 cggggccggc gtcgagttcg gccgggcgca gggtgggttc acccggatcc tgcagacgca
    16441 aggcggcaac cagttcgagg gactcgtcga gctgggctat cgcgtcccgg tcggcgatgg
    16501 agcgggctgg aacggctcgt cgcgaccggg ctggcaggcc gggatccagc tttccggacc
    16561 gatcgtcaag aaccgcctgt ggtatcgcct gtcccacgac gggctcgacc gagaccagcc
    16621 gatcgacgac acgcggagcg ttaccgcggt tccgacgagg gcatcggctc actcggaccg
    16681 catcacctgg caggtctcgc cgcggaacaa gctcaccgtc gatctggcga aggagcgcgt
    16741 aagcctcgac aacgtggggt tgagctcgtg gatcggaccg gactcgacgc gatcggtccg
    16801 cagcggcggt ccgcgctacg tcctgtcctg gatcgttccg tactcgccga agatcctggt
    16861 ccaagccagc gtcgcctacc agtcgagcga gttcgaggtc gtgccgtcgc gaccgggcgc
    16921 agccaacgat tgcatcgcgt cgctcgccgc ggagccggat cgaaccgcgt gtctcgatct
    16981 gacggccgcg gtcgtgtccg gaccggcgca ccacgaatgg gagcaggacc gcagtcggct
    17041 ttccgtctcg ggacagtcga cgatctacgc cggtcgggcg atcggcgcca cgcaccagct
    17101 cgaactcggg ttcgaggtgc acgacgatcg tttcgatcga acgatagtcc gggggccgac
    17161 gctgattcga gggagcgata ccgacgggtc cgggcgcctc accggccgaa tcgccgacga
    17221 cccgatcggg cgggatgggg cgcgggggac gctctggggc gtgtgggctc aggatcagct
    17281 caaaccgagc cagaacctga cactgacgct ggggctccgg gccgatcgct acgaattgga
    17341 ggcggtcggc tacgccccgt tcgatccgaa tctgcccgcg gaggcgtcgg cacgccgcgc
    17401 tcagaacttg ctcatcgcgg cgacgaacgt ttcgcctgcc ctcagcgtcg cgtgggatcc
    17461 gtggatggac ggcaagtcga agttcttcct ctccgcgaaa cgggtccacg acgagctgag
    17521 tccggccgtt ctgctgatcg agcaggagcc tttgacccgt gacgtcgacc tgacacgggt
    17581 cacggtcgat ccggagagag cgaccgggcc gcagaacgtg cagatcgtcg atcgcgacct
    17641 cgaggcgccg tacctggacg aatggaccgt cggcttcgag cgccagatct tcaccgagac
    17701 gagcatccgc gcgacgctga tcgcccggga gtaccgcgac cggctcggct tcgtcgatct
    17761 caatcggacc ctcctgtcgc cgtattgggg cgagatcgtc tcgatcggca acctccattc
    17821 cgcgcgctac cgtgcgtttc gactcgagtg gacgcgccgc cagtaccgca actgggagct
    17881 tcaggcggac tacacctact ccgtgctcga gggcgatgcg caagatctcg acttgctggt
    17941 gggcgacgag cgttcccggt tcgaggtcgc gccgggttac caggccggtg accagcgcca
    18001 ctttttccga gcggatgcga cgtggatcac cccgtggggt atccgcctcg gtggcgcgct
    18061 ccgagtcggg tcgggtctac cgttctcccg ggtcgagtcg agctggcgcc cggtcggcga
    18121 gaccgccgta ccggtgccgc gattcgtcca cccctccggg cggcgcaacg atctgcgcaa
    18181 ccctccgacc tggaccgtcg acttccgcat ggcgcgtgac ttctccgccg gacgcgcggt
    18241 cgacatccgg gtggcgctcg aggtgttcca cctgttcggc aacgacccgt atctcgtgta
    18301 caacgacgcg cgcggctccg gacagcgcat cgacggcgtg gacgagctca cccggttctc
    18361 acgacgcgag gtccagctcg gcgtcgagct gcgtttctga gggccgcgcg ccgaccgcgc
    18421 gcacgggatc gttgctcggc cggccgcgcg ccccatattg gacgtatgcg actcacgccg
    18481 gctgccttcg cactcggttt ggcgctcgcc gggcccgcga tcgcggatga cggcgcgcgc
    18541 tg
//
LOCUS       DASPGN010000027         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1059340, whole genome shotgun sequence.
ACCESSION   DASPGN010000027 DASPGN010000000
VERSION     DASPGN010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059340"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /gene="ruvA"
                     /locus_tag="VD788_00815"
     CDS             <1..372
                     /gene="ruvA"
                     /locus_tag="VD788_00815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HXV74831.1"
                     /translation="ALAFLSGIGADELRDCVDRQDRARLQRIPGVGRKTADRVLLELR
                     DKLGDRPGEVAGAEHRTAGAGRAGSERTAILDDAVSALVNLGYTRETAARVVERAAAA
                     VDGELPTLEQLLKRALATLAG"
     gene            398..1390
                     /gene="ruvB"
                     /locus_tag="VD788_00820"
     CDS             398..1390
                     /gene="ruvB"
                     /locus_tag="VD788_00820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXV74832.1"
                     /translation="MSGLVDAGEDAYERTLRPRRLDEYVGQPQIKANLDVFIRAARQR
                     GEPLDHVLLYGPPGLGKTTLAHVIANEMDAPVRITSGPSIEKAGDLAAIVTNLQHGEV
                     LFVDEIHRLGRTLEEILYPAMEDGVLDLVIGNGPGARSVRLELPRFTLIGATTRFGLL
                     SPPLRDRFGIIHHLDYYGVEELAYIARRSAGILGVGVDEPGAVEISKRSRGTPRVTNR
                     LLRRVRDFVQVAGGETITRDAAVDALERLAVDRFGLDPLDRKILGIVLDKFDGGPVGL
                     STIAAAVGEDAGTIEEIYEPYLMQIGFLDRTPRGRRVTRQARAHLDRPEPPSLF"
     gene            complement(1469..>3230)
                     /locus_tag="VD788_00825"
     CDS             complement(1469..>3230)
                     /locus_tag="VD788_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74833.1"
                     /translation="FDGTTTPQSITLDLDLDASGGVCNTPPCMFEEGWENLPNDNCAD
                     DGGTACRGFQAINKDFDLQDLVSADGYRCQYHDPDWPNSLAYQGGVTDCFLGEGSAHA
                     ARFWWQINTPADPNGGKRYSGQNSLYMGDFGTGPDTQTTPISVLEFLQMIDPIPLGLA
                     GDPPELSFVHQVDFMDDRNVNVLPGQSPARGIVSLQLADTDGDPVGNWIKLQPYLNIY
                     DTQAQDQYFNCSFDPIDDGNDEDSFFDPTDPDRRLGPSTSCSPEMAFVYMGETFSTFS
                     EGNLAKAEGPGLAGADLKGTWVESKFNLERFRGRSVRLRFMYTDLKTSAGTTWESLFA
                     LNPGPGDDGWWIDDLRVTNTLDTPADILNDDKDNSGLPASCGTNCTTITMAVTRDPVS
                     TLTAPGQVVEFDATNTSANRCLDGTLQYQFWRDGNANNMGGDPLDTLLRGWTDNPVLI
                     DAPTSTTNYVVEARCSTATTCVNRSAINVAVNCPASGALVFPTITSLDGDSLMWTGSE
                     TYNWARGNLITLDGSYTPTGSAIGVGPGTSFSISGDVVAPGAGNWYLFRRPGALGGGG
                     GFCNDLGVSWGTLLRDANLP"
BASE COUNT          518 a         1016 c         1146 g          550 t
ORIGIN      
        1 gcactggcgt tcctgtcggg aatcggcgcg gacgagctac gcgactgcgt cgatcggcag
       61 gatcgcgctc gcttgcagcg catcccgggt gtcggcagga agacggcgga ccgcgtgctg
      121 ctcgagctgc gcgacaagct cggcgatcga cccggtgagg tcgccggcgc ggaacacagg
      181 acggccggtg ccggtcgcgc cggcagcgag cgaaccgcga tcttggacga cgcagtctcg
      241 gcgctcgtca acctcgggta cactcgggag acggcggcga gggtcgtcga gcgcgccgcg
      301 gccgcggtcg acggggagct accgacgctc gagcagttgc tcaagcgggc actggcgacg
      361 ctcgcaggat gatcgcgctg tgtcggacga acggatcgtg agcggattgg tggacgcggg
      421 cgaggacgcc tacgagcgta ctctcagacc gcggcggctc gacgagtacg tcgggcagcc
      481 acagatcaag gcgaacctcg acgtcttcat ccgggccgcg cgtcagcgcg gcgaaccgct
      541 cgaccacgtc ctgttgtacg gtccgccggg tctgggaaag accaccctgg cacatgtcat
      601 cgccaacgag atggatgccc ccgtacgaat cacgtcgggg ccgtcgatcg agaaggcggg
      661 ggatctcgcc gcgatcgtga cgaatctgca gcacggggag gtgctgttcg tcgacgagat
      721 ccatcggctg ggtcggacgc tcgaggagat cctctacccg gcgatggaag acggtgtgct
      781 cgacctggtg atcggcaacg gtccgggcgc ccggagcgtg cgtctcgagc tgccccgctt
      841 cacgctgatc ggcgcgacga cgcgcttcgg gctgctctcg cccccattgc gcgatcgctt
      901 cggcatcatc catcatctcg attactacgg ggtcgaggag ctggcgtaca tcgcgcggcg
      961 ctcggccggg atcctcggcg tgggggtcga cgagccgggc gcggtcgaga tctcgaagcg
     1021 gtcgcgcggc acgccgcggg tgacgaaccg cttgttgcgg cgcgtgcgcg atttcgtcca
     1081 ggtcgccggc ggcgaaacga tcacccgtga cgcggcggtc gatgcgctcg agcggctggc
     1141 ggtcgatcga ttcggtctcg acccgctgga tcgcaagatc ctggggatcg tcctcgacaa
     1201 gttcgacggc gggccggtgg gactgtcgac gatcgccgcc gccgtcggcg aggatgccgg
     1261 cacgatcgag gagatctacg agccctatct gatgcagatc ggctttctcg atcgaacccc
     1321 gcgcggccgg cgggtcacac ggcaggcccg ggcccacctc gaccgccccg agcccccgtc
     1381 gttgttctga ccgcgacgag cggaaccaaa aaaagagggg agggaccctc ggtccctccc
     1441 ctcggtttgg cgcttgttgc gttgcgcgtt acggcagatt cgcgtctcgc agcaacgtac
     1501 cccagctgac gccgagatcg ttgcagaagc cgccgcctcc accgagagct cccggtctgc
     1561 ggaacaggta ccagttgcca gcaccggggg cgacgacgtc gcctgagatg ctgaacgacg
     1621 tcccgggacc gacgccgatc gccgagccgg tcggcgtgta gctgccatcg agcgtgatca
     1681 ggttaccccg cgcccagttg taggtctcgc tgcccgtcca catcaagctg tcgccgtcga
     1741 gcgaggtgat ggtcgggaag accaaggcgc cgctggccgg acagttgacg gcgacgttga
     1801 tcgcgctccg gttcacgcac gtcgtcgcgg tcgagcaacg cgcctcgacg acgtagttcg
     1861 tcgtcgaggt cggagcgtcg atcagcaccg ggttgtcggt ccatccgcgc agcagcgtgt
     1921 cgagcggatc gccacccatg ttgttggcgt tcccgtcccg ccagaattgg tactggaggg
     1981 taccgtccag gcaacgattg gcgctcgtgt tcgtcgcatc gaactcgacg acctgaccgg
     2041 gcgccgtcaa cgtggagacc gggtcgcgtg tgaccgccat cgtgatcgtg gtgcagttgg
     2101 tgccgcagga cgccggcaag ccgctgttgt ccttgtcgtc gttcaggatg tcagccggcg
     2161 tgtcgagcgt gttggtcacc cggaggtcgt cgatccacca gccgtcgtca ccggggccgg
     2221 ggttcaacgc gaacaacgat tcccacgtcg tgccggcgct cgtcttgagg tccgtgtaca
     2281 tgaaccgcag gcgaacgctc cgaccgcgga accgctcgag gttgaacttg gactcgaccc
     2341 aggtgccctt gagatcggca cccgccaggc ccggaccttc cgccttggcg aggttgccct
     2401 cgctgaaggt ggagaacgtc tcgcccatgt agacgaaggc catctcgggc gagcacgagg
     2461 tggacggacc gaggcgccga tcgggatcgg tcgggtcgaa gaacgagtct tcgtcgttgc
     2521 cgtcgtcgat cgggtcgaag ctgcagttga agtactggtc ctgcgcctgc gtatcgtaga
     2581 tgttgagata cggctggagc ttgatccagt tgccgacggg atccccgtcg gtgtcggcca
     2641 actgcaacga gacgatgccg cgtgccgggg actgacctgg aagcacgttg acgttgcggt
     2701 cgtccatgaa gtcgacttgg tggacgaacg acagctccgg aggatcgccc gcgagaccga
     2761 gcgggatcgg atcgatcatc tggaggaact cgaggacgga gatcggagtg gtctgggtgt
     2821 cggggccggt gccgaaatcg cccatgtaga gcgagttctg cccgctgtag cgcttgccgc
     2881 cgttggggtc ggcaggtgtg ttgatctgcc accagaagcg cgcggcgtgg gcgctgccct
     2941 cgccgaggaa gcagtcggtg acgccaccct gatacgccag cgagttcggc cagtcgggat
     3001 cgtggtactg gcagcgatag ccgtcggccg agaccagatc ctggaggtcg aagtccttgt
     3061 tgatggcctg gaagccgcgg cacgccgtcc cgccgtcgtc ggcgcagttg tcgttgggca
     3121 ggttctccca gccctcctcg aacatgcagg gcggagtgtt gcagacgcca ccgctggcat
     3181 cgaggtcgag gtcgagcgtg atgctctgcg gcgtcgtcgt cccgtcgaac
//
LOCUS       DASPGN010000028        12321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_356408, whole genome shotgun sequence.
ACCESSION   DASPGN010000028 DASPGN010000000
VERSION     DASPGN010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12321
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356408"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2678)
                     /locus_tag="VD788_00830"
     CDS             complement(<1..2678)
                     /locus_tag="VD788_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXV74834.1"
                     /translation="MRIGALAIVSLLSVTAAQSAVIRVAPGGDDANNGSTWTEAKRTI
                     GAAIGAANTGDEIWVAAGTYAETIRNRTSGGIAVDVALYGGFAGNEATRDERDFVARA
                     TIIDGGGLGSVVTIDALAGPDMRVDGFRIRNGQATFGGGVQVTGAAPTITNNDVLGNQ
                     ADHGGGIMVWGYRTIPPVAHARIVSNVIQVNRGGSGGGGVAVVGASPEIRGNTILRNT
                     TGGYGGGIGVWVSESSKVARPWIANNFIFENAANRTTAGMTVGGGGIFATERNIGGEP
                     VSFGICTPRIEDNLIAANAAIACGGGIAIVNADTESAPITNNTVVANSGSGICWGNSG
                     PTIVNNIVALNTWGVEEDVGNPVAETIRFNDVWGNSVHGENTDYFQLADRSGIDGNIS
                     TDPLLVKYGAWRLRLQPGSPCSDAGDDSVVAGGREDVDGQPRILGAHVDIGADEQDGT
                     QWAEIPGIVRVRPGGNDAADGSSWELAKATLQGAIDSVWAAGGGEVWAAQGTYAEHPI
                     LSAWVGLYGGFAGNETERGQRDPQTHVAEIDGGGVPPVISCGLSGYRVGAIDGFRITG
                     GGNYTGGTTLPPPSSPAGQGGGIRCAVSSPVIRGNEIAGNSLGDPNTAPFEGPGVGGG
                     ISLVGSHALIDGNIFADNEVLNRSSVGGAVYCEWSVVDLAHNTIGRNHAPEGAAVYCT
                     AARPLLFNNLISQNENYYWPPLYFGSTTGAVSLHLCWDLDVVFNYFVGNIADAGGALY
                     LDQPRRGTVANNLFVGNHAYLRQLSSGGEGRAIWLLVGSAPEEALSVSGNTFAGNTAT
                     SFYSGELGGAIAVLPLSAMATIANNVMAFNSSGIYQRPGFATNPVLSRNDLYNTGANY
                     VGLPPGGTDLELDPLFADAANGDYRLQSVSPLVDEG"
     gene            complement(2826..3716)
                     /locus_tag="VD788_00835"
     CDS             complement(2826..3716)
                     /locus_tag="VD788_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74835.1"
                     /translation="MMLPNRDLVGSTLAGLLATLSAGVATAEVSVRVVDDDCASALIS
                     VIIEDADPVGWSVWSSPKQYAETTTVINPTGEIRGDLFPDVIFDPDACVVQLTWPYRV
                     GPDYTDVAFMEVTNGVIGPYVFLTSDTQVESDPRVFTATDGSARVVWWVAGTPDAVYL
                     AARPGDASQWEPNLRVTPETEGGRRPSAAIFDGTTYVAYERPVGSVTELVVAPMSPDG
                     TFAGELVATAGELPLDPVLHVDDGAMWLDWRQDLDTVGYAVFVDGVWSDPFQIPRPGG
                     SWLEMEAARKTVRRTVLQGD"
     gene            complement(3713..4867)
                     /locus_tag="VD788_00840"
     CDS             complement(3713..4867)
                     /locus_tag="VD788_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV74836.1"
                     /translation="MGNFVRSVREAQNLSQDEVAEITQGQPWALSRSAISGIERGRSL
                     PSVEALVGLSRALHFDPNEVIERVERALHPGVTSPSEQADERRAEELFWAGRHRDALM
                     LYDALIAAVEARDEAGDSATTEKRALYEIRRAACLRRCGMLLSAKAAAERGVSLAAGH
                     PSLQADGYLVLSATHRQRGNLPLAKDMADRAVRLTRGSDSPGEARALLQAGACLFDLG
                     RFREALRTLLEARKSAVKHRDHRHLSHISGNIGMCWVGLDDRARAKRWVRRAYLLARE
                     HELPALEASWLVELGRFSLDEGRTDDAAGYARAAVDIARSRQNWLTLLRAEWLRHLVK
                     QRTSPGRADRRRIVRLRALLQRLTPHATDPTVKEVVGYLRAGLQNDRGER"
     gene            5156..5338
                     /locus_tag="VD788_00845"
     CDS             5156..5338
                     /locus_tag="VD788_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74837.1"
                     /translation="MRKIKATLVTAMLLAGSTAAFAQPEVPDTPTDPSGIEGFVMALL
                     ENVRAFVEGLIGTLPV"
     gene            5404..8010
                     /locus_tag="VD788_00850"
     CDS             5404..8010
                     /locus_tag="VD788_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV74838.1"
                     /translation="MPLLTAVVLSALIAPSIPAAPAARCDAAAVGLAVGCVPVRDVTP
                     RSDSSGAPRYHIARFRSVGELADFRRDHREIVLDYLPVNALVVRLTAELAAEIAAHPG
                     LDGLEPYRSSYKLHPALTRPGARGGLAPNGRLAVALFEDDSVDAVVRSVRSRGIEVEQ
                     IVVSPYRVTLYLVAAEPVWDAELSRLTREEAVRFIEPAPWPRTMNSNTDEVLQGGFVG
                     AGRPYRDAGITGAGQEASVMDSGLDVDTIWFADLPTDAGVPGPGHRKVASYVAWGQGT
                     IGTCSCVDISPIPAQPFSLSHGTAVTAGILANRTDFGLTDVVEGMAPGARVHFQDIGD
                     SLGGAGTCGGGALYPPSDLTLPLVDASNRGARVHNLSWGTIQGDTYSASAIDVDAFLW
                     THPESVVVLPGGDCGPEGGAQCPSPGFASIGTPATAKNDITVGGADQDPIQDDVDSSS
                     SRGPETVTSGRTGPTVTMIDTDTVRAHAIASVNAPQDLGANCTFATGDCDIEGAPGAA
                     CVAFGLSGTSYATGFTSGAALLVRDYLARGFYPSGAANAGNELTGPSGAAIKALIVNS
                     AEFMTGAGAGTDRFNNDQGYGRVLLSNTLPLANDAATPAGLALWDFGRAVGLDGSEPE
                     RYHLFEVIDTSREVRVTLAYYDAPSPAGSAGALINDLSLEITSPDGKSYRGNHFSGAS
                     TPADAGVEDSLNPTEMVVIDPNALVPGRYVATVRVGAAGVPEPHPSFGGQPYALVATG
                     GLVQRDLPHLGEVDDGAGSAAAPLVAGFDGTQVVLDWSASCLPGDYIVYKGTLASLSG
                     SSTSQATVLAASTGGLPGATDAALEGNHYYVVAPHDGAFEGSYGRDSDLAERPQSVNS
                     PFLRSLEPCSPP"
     gene            complement(8052..10691)
                     /locus_tag="VD788_00855"
     CDS             complement(8052..10691)
                     /locus_tag="VD788_00855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV74839.1"
                     /translation="MIDPARWERVQALFHRVADRPAAERAAILDAERAADPTLVAEVE
                     GLLEEDARAGSALDRDLAHVAARVLGSPVPHGTFGPYRLGGLLGEGGMGVVYRAERDD
                     LGSVAAIKILRDAWLSPARRERFRAEQRTLAQLNHPSIAQLHHADALPDGTPWFVMEY
                     VEGVPLTDYCREHGTSIEGRLELFRAVCEAVQYAHGQAVIHRDLKPSNILVKADGTVK
                     LLDFGIAKHLASEHQPIDQTRTALRMMTPAYASPEQLRGERVGIHSDIYSLGVILYEL
                     LTGRPPFDLSGLTPAEAISRLVDHDPERPSVVAARVTASPIERPRFRPGRYGSWSDLD
                     VLCLTAMHRDPLRRYPTVDALIGDVGRYLAGEPLLARPGVVGYRIGKFIRRHRVSVVA
                     AVGIAAVLVGMATYYTTRLARARNEAVAEAARTQRIQRFLTNLFEGGDEAAGPAAEMR
                     VVTLIERGVIEARSLAAEPVIQAELYETLGRIYHKLGDLDRADELLEASLERRRELPG
                     DRRIDEAESRIALGLLRSDQAQYDEAETLCREGLATLVAARPRDHPSIAAAQTALGHV
                     LIERGSYDDAAANLELAAAIRERHREETPELADTLFELANAHFYRGRLAEAEAVMGRV
                     LVLSRGLYGDRHPHVAEDLVNLGAIRHEQGDYAGAEQHYRDALEINREWYGDDHPATA
                     SAQIMLGRSLVYAERFDEAQRELSEALATQERVYGPDHPRVASALNDLGSIALQRDDY
                     PAAEAAFRRTVAIQRAAHPQGHYLVGTAVSNLASVFMARGHWTEAEPQFREAIEIYVA
                     TLSAEHLNVGIARIKLGRVLLRQGRHEEALAQTLAGHETLTRQTDPAVSWLRAAREDL
                     VAEYDALGRAAEADAYRAPVASP"
     gene            complement(10688..11242)
                     /locus_tag="VD788_00860"
     CDS             complement(10688..11242)
                     /locus_tag="VD788_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HXV74840.1"
                     /translation="MVDESNDDPFDAAVHDRETLDLLFGATYEELRRLATTVRRGDPS
                     ATLNPTALVNEAWFKLAQSPPVGTVSRLHFKRIAARAMRQVLVEAARRRKTDKRGGGL
                     APVTFDESLGAVDVADDLLALDASLAALARVNPRQAAIVESRYFGGLEVGETAGLLGV
                     SEATVLRDWRAARAWLAREIGRRS"
     gene            11414..11725
                     /locus_tag="VD788_00865"
     CDS             11414..11725
                     /locus_tag="VD788_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74841.1"
                     /translation="MQIERSPFLSLDRSQVIGYFKAAGSRDPDVLHTHKASLISLAKF
                     PKLAGTYLMVVGGLCTLLILLAPIGLPLLGLGWWTRRRGVRNLETVESGFTEFMRGAA
                     A"
     gene            complement(11748..>12321)
                     /locus_tag="VD788_00870"
     CDS             complement(11748..>12321)
                     /locus_tag="VD788_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HXV74842.1"
                     /translation="LLLVLVLDPFGNVPFFIVALDRVEPARRRIVLVRELLIALAALV
                     AFLFVGEHVLALLQISEPSLGVAGGVILFLIALRMIFPRARVDLDEEADSEPFVVPLA
                     IPYVAGPSALASVMFIMNREPERWPVWLAATVLAWLVTASTLYFAQGLRRMLGRRGLI
                     AVERLMGMVLITLAVQMMLNGLAAFLGDAA"
BASE COUNT         1847 a         4345 c         4195 g         1934 t
ORIGIN      
        1 tctccttcgt cgaccagcgg ggagacggat tgcaggcggt agtcgccgtt ggccgcgtcg
       61 gcgaacaacg ggtcgagctc taggtccgtg ccgccgggcg gaaggccgac gtagttcgcg
      121 cccgtgttgt agagatcgtt gcgtgacagc acgggattcg tggcgaagcc aggccgctgg
      181 tagattcccg acgagttgaa cgccatcacg ttgttggcga tcgtcgccat cgccgacagc
      241 ggaagcacgg cgatcgcacc accgagctcg ccgctgtaga agctcgtcgc ggtgttaccc
      301 gcgaacgtgt tgccggacac cgacaacgcc tcctccggag cgctgccgac gaggagccaa
      361 atcgcgcggc cctcaccgcc gctcgacagc tgccgcaggt aagcgtggtt tccgacgaac
      421 aggttgttgg ccaccgtgcc gcgccgaggc tgatcgaggt agagcgcccc accggcgtcg
      481 gcgatgttcc cgacgaaata gttgaagacc acgtcgagat cccagcacag atggagcgag
      541 accgcgcccg tcgtcgaacc gaagtagagc ggcggccagt agtagttctc gttctgcgag
      601 atcaggttgt tgaacagcag tggccgcgcc gccgtgcagt agaccgcagc tccctcgggc
      661 gcgtggtttc gtccgatcgt gttatgcgcc aagtccacga ccgaccactc gcagtagacc
      721 gcgccgccga cgctcgagcg gttcagcacc tcgttgtcgg cgaagatgtt gccatcgatc
      781 agcgcgtggc tgccgacgag gctgatccca ccgccgacgc ccggcccctc gaacggtgcc
      841 gtgttcggat caccgagcga gttgcctgcg atctcgttgc cacggatgac cggcgagctg
      901 acggcgcaac ggatcccgcc accctggccc gccggcgagg acggcggagg cagcgtggtg
      961 cctccggtgt agttaccgcc gcccgtgatg cggaagccgt cgatggcgcc gacacggtag
     1021 ccggacagtc cgcacgaaat gaccggcggc actcccccgc cgtcgatttc cgcgacgtgg
     1081 gtctgcggat cgcgctgacc gcgctcggtc tcgttgcccg cgaagccgcc gtagagaccg
     1141 acccaggcgg agagaatcgg atgctcggcg taggttccct gggcggccca gacctcaccg
     1201 ccgcccgccg cccagacgct gtcgatcgcc ccttgcaacg tggctttggc cagttcccac
     1261 gaggagccgt cggcggcgtc gttgcctccg gggcgcacgc ggacgatccc cggaatttcg
     1321 gcccactggg tgccgtcctg ctcgtcggca ccgatgtcga cgtgcgcgcc gagaatccgc
     1381 ggctggccgt cgacgtcctc gcgaccgccg gcgacgaccg agtcgtcacc ggcgtccgaa
     1441 cagggtgacc ccggctgcag tcgcaggcgc cacgccccgt atttcaccag cagcgggtcg
     1501 gtcgagatgt tgccgtcgat tccgctccgg tccgcgagtt ggaagtagtc ggtgttctct
     1561 ccgtggaccg agttgcccca gacgtcgttg aaacggatcg tctccgccac ggggttgccg
     1621 acgtcctcct cgacacccca cgtgttgagc gcgacgatgt tgttgacgat cgtcgggccc
     1681 gagttgcccc agcagatgcc cgaaccgctg ttggccacca ccgtgttgtt cgtgatcgga
     1741 gccgactcgg tgtcggcgtt gacgatcgcg atgcctccac cgcaggcgat ggcggcgttg
     1801 gccgcgatca ggttgtcctc gatccgcggc gtacagatgc cgaagctgac cggctcacca
     1861 ccgatgttgc gctcggtcgc gaagatcccg cccccgccga cggtcatgcc ggccgtcgtg
     1921 cggttcgccg cgttctcgaa gatgaagttg ttcgcgatcc acggccgagc gaccttcgac
     1981 gattcgctga cccagacgcc gatgcccccg ccgtagccgc cggtcgtgtt gcgcaggatg
     2041 gtgttgccgc ggatctcggg cgaggcgccg acgaccgcga ccccgccgcc gccgctgccg
     2101 ccgcggttga cctggatcac gttcgagacg attcgcgcgt gcgccaccgg cgggatcgtg
     2161 cgatagcccc agaccatgat cccgccgccg tggtcggcct ggttgccgag gacgtcgttg
     2221 ttggtgatcg tcggtgccgc accggtgacc tggacgccgc cgccgaacgt cgcctgtccg
     2281 ttgcggatcc ggaagccgtc gacgcgcatg tcgggaccgg ccagcgcatc gatcgtcacg
     2341 accgaaccga gcccgcctcc gtcgatgatc gtcgcccgtg cgacgaagtc gcgctcgtcc
     2401 cgcgtcgcct cgttgccggc gaaccctccg tagagcgcga cgtcgaccgc gatgccgccg
     2461 gacgttcggt tgcggatcgt ctccgcgtag gtcccggcgg cgacccagat ctcgtcgccc
     2521 gtgttcgccg cgccgatcgc cgccccgatc gttcgcttcg cctccgtcca ggtcgatccg
     2581 ttgttcgcgt cgtcacctcc gggcgcgacg cggatcaccg ccgactgcgc cgcggtgacc
     2641 gacagcagcg acacgatcgc aagcgcaccg attctcatgg ggtcccccgc ggtgggggat
     2701 caggtcccgt gcgtaggagg cgggcccgcc cggtctcccc cgcccgacgt ccaccgggcc
     2761 gggagtggca cgatcataac gcgcgcgcgt cgggggtgcc cgccggtcga ggagccagat
     2821 gatcctcaat cgccctgaag aacggtgcgc cggaccgtct tgcgggctgc ctccatctcg
     2881 agccaggacc cacccggccg cggtatctga aacgggtcgc tccaaacgcc gtcgacgaac
     2941 acggcgtagc cgaccgtgtc gaggtcctgt ctccaatcca gccacatcgc tccatcgtcg
     3001 acgtggagga cgggatcgag cggaagctcg cccgcggtcg cgacgagctc gcccgcgaac
     3061 gtcccgtcgg gcgacatcgg cgcgacgacg agctcggtca cgctccccac cggccgctcg
     3121 tacgcgacgt aggtcgtccc gtcgaagatg gcggcggacg gacggcgccc gccctccgtc
     3181 tccggcgtga cccgcaggtt cggttcccac tgactcgcgt caccggggcg tgcggcgagg
     3241 tagacggcgt cgggtgttcc cgccacccac cacacgaccc gcgccgaccc gtccgtcgcc
     3301 gtgaacacgc gcggatcgga ttccacctgc gtatcgctcg tcaggaacac gtagggcccg
     3361 atcaccccgt tcgtcacctc catgaaagcg acgtcggtgt agtcgggtcc gacgcggtac
     3421 ggccaggtca gctgcacgac gcatgcgtcg ggatcgaaga tcacgtcggg gaacaggtca
     3481 ccgcggatct ccccggtggg gttgatcacg gtggtcgtct cggcgtactg cttcggcgat
     3541 gaccaaacgg accacccgac cggatccgcg tcctcgatga tcacggatat cagcgccgac
     3601 gcgcagtcgt catcgacgac acggaccgaa acctcggcgg ttgccacgcc ggcggacagt
     3661 gtcgcgagca gcccggccag cgtcgagccc acgaggtcgc ggttcgggag catcatcgtt
     3721 ctcctcgatc gttctggagt ccggctcgca ggtagcccac gacttccttc accgtcgggt
     3781 cggtcgcatg cggggtcagc cgttgcagca gcgcgcgcag ccgaacgatc cgcctgcggt
     3841 cggctcgtcc cggactcgtc cgctgcttga cgagatgacg aagccactcg gcgcggagca
     3901 aggtcaacca gttctgtcgc gacctcgcga tgtcgaccgc cgcccgagcg tagcccgcgg
     3961 cgtcgtccgt ccgtccctcg tcgagcgaga agcggccaag ctcgacgagc cagctcgctt
     4021 cgagggccgg caactcgtgc tcgcgcgcca gtaggtaggc acgtcgaacc catcgcttgg
     4081 cacgggcacg atcgtcgagc ccgacccaac acataccgat gttgccgctg atgtgcgaga
     4141 gatggcggtg gtcgcgatgc ttcaccgccg acttgcgggc ctcgagcagc gttcgcagcg
     4201 cttcacgaaa acggccgaga tcgaacaggc aggcgcccgc ttgaagcagc gcgcgggctt
     4261 caccaggtga atcgctgcca cgggtcagcc gtaccgcacg gtcggccatg tccttggcga
     4321 ggggcaggtt tccccgctgc cgatgggtcg cggacagcac gagataacca tcggcctgca
     4381 gcgacggatg gccggcggcg agcgacacgc cgcgctccgc cgcggccttg gccgacagca
     4441 gcatcccgca tcgacgcagg caggctgccc ggcgaatctc gtacaacgca cgcttttcgg
     4501 tcgtcgccga atcgcccgcc tcatcccgcg cctcgactgc ggcgatcaac gcgtcgtaga
     4561 gcatcagcgc gtcgcggtgg cgaccagccc agaacagctc ctccgcgcgt cgttcgtcgg
     4621 cttgctcgct cggcgacgtg acgccggggt gtagcgctcg ctcgacgcgt tcgatcactt
     4681 cgttcgggtc gaagtgcaga gcccgcgaca gtccgacgag cgcttcgacg ctcggcaagc
     4741 tccggcctcg ctcgatgccg ctgatcgcgg aacgcgagag cgcccacggt tgaccctgtg
     4801 tgatctcggc cacttcgtcc tgactcaagt tctgtgcttc gcggacggag cgcacgaaat
     4861 tccccacgac gaccgtgacg tctttggacg tggttcgacg gcgcggtttc ttccgagcca
     4921 actacccctc ccgtcgcggt cgcggcaccc gccgcttcac tgcttgtacg gcgccgcgac
     4981 gtctggtgtc aaggaatcta acacgcgtcg cgccgcgtgc acggtcgcta catttgcgtc
     5041 ggatccgatc gctacatctg cgtccgcatc gattccaaat tgcgttgcgt cctcgagtct
     5101 cccccgtact cttccccgcg agagaaggcg actcgaaaca aaggaggacc gtgctatgcg
     5161 taagatcaag gccacgctgg tgaccgcgat gctgttggcg ggttcgaccg ccgcgttcgc
     5221 gcaaccggaa gtacccgata ctccgacgga cccgagcggc atcgaaggtt tcgtgatggc
     5281 cctgctcgag aacgttcggg cgttcgtcga aggactgatc ggcactctcc ccgtctgagg
     5341 cgaaccggcg aaaccgccgt ggcgagggct ccggctcgcc ggctgcgggc cggagcactc
     5401 gtcatgccgc tgctgaccgc tgtcgttctc tccgcgctca tcgctccctc cattcccgct
     5461 gcgcccgccg cgcgctgcga tgcggccgcg gtggggctgg ccgtcggttg cgttcccgtg
     5521 cgagacgtca cgccgcgcag cgattcctcg ggtgctccgc gctaccacat cgctcgcttc
     5581 cgctccgtcg gggagctcgc cgacttccgc cgcgaccatc gcgagatcgt cctcgattac
     5641 ctgcccgtga acgcgctggt ggttcggctg accgcggagc tcgcggcgga gatcgccgcg
     5701 catcccgggc ttgatggact cgagccgtac cgctcgtcgt acaagctgca tcccgcgttg
     5761 acccggcccg gcgcgcgcgg cgggctcgcg ccgaacggcc ggctcgctgt ggcgttgttc
     5821 gaggacgatt cggtcgatgc cgtggtgcga tcggtgcgga gtcgtgggat cgaggtcgag
     5881 caaatcgtcg tcagtccgta ccgcgtcacg ctctacctcg tggccgccga accggtgtgg
     5941 gacgccgagt tgtcgcgcct gacgcgcgag gaggccgttc gcttcatcga gcccgcgccg
     6001 tggccgcgca cgatgaactc gaatacggac gaggtgctgc agggcggctt cgtcggtgcc
     6061 ggcagaccgt atcgggacgc cggaatcacc ggagcagggc aggaagcatc ggtgatggac
     6121 tcggggctcg acgtcgacac gatctggttc gccgatttgc cgaccgatgc gggcgtgccc
     6181 ggccctggcc atcgcaaggt cgcgtcatac gtggcttggg gccagggtac gatcggaacg
     6241 tgttcgtgcg tcgacatctc gccgattccc gcacaaccgt tctcgctctc ccacgggacc
     6301 gcggtgaccg ccggcatcct ggccaaccgc accgacttcg ggctgaccga cgtcgtcgaa
     6361 gggatggcac cgggcgcgcg cgtccacttt caagacatcg gcgacagcct gggcggcgcc
     6421 ggcacgtgcg gcggcggcgc actctatccg ccgtcggacc tgacgctgcc gctcgtcgat
     6481 gcgtcgaacc gcggagctcg cgttcacaac ctcagctggg ggacgatcca gggggacacg
     6541 tactccgcgt cggcgatcga cgtcgacgcc tttctctgga cacatccgga atcggtggtc
     6601 gtccttcccg gcggcgactg cggtccggaa ggaggtgcgc agtgcccgtc cccgggcttc
     6661 gccagcatcg gcacgccggc gacggcgaag aacgacatca cggtaggcgg cgctgatcaa
     6721 gatcccatac aggacgacgt cgattcctcg tcgagtcgtg gcccggagac cgtgacgagc
     6781 ggtcgcactg ggccgacggt gacgatgatc gacaccgaca cggtgcgggc gcacgcgatc
     6841 gcgagcgtca acgcgccgca ggacctcggt gcgaactgca ctttcgcgac gggcgactgc
     6901 gacatcgagg gagctccggg ggcagcttgc gtcgccttcg gtttgtcggg gacgagctat
     6961 gccaccgggt tcacaagcgg cgcggcgctg ttggtccgcg actacctggc ccgtggcttc
     7021 tacccgtcgg gcgcggccaa cgccgggaac gagctgaccg gtccgtcggg cgcggcgatc
     7081 aaggcgctga tcgtgaacag cgccgagttc atgacgggcg ccggcgccgg gaccgaccgc
     7141 ttcaacaacg accaaggata cggtcgcgta ctgctgagca acacgcttcc gctcgcgaac
     7201 gacgcggcga ccccggcagg tctcgcgttg tgggacttcg gccgcgcggt cggtctcgac
     7261 gggagcgagc ccgagcgcta tcacctcttc gaggtcatcg acacctcgcg ggaagtccgg
     7321 gtcacgctcg catactacga cgctccgtcc ccggccggtt cggcgggagc gttgatcaac
     7381 gacctgtcgc tcgagatcac ctcaccggac ggcaagtcgt accgtggcaa tcatttctcc
     7441 ggcgcctcca ccccggccga cgccggcgtc gaagacagcc tcaatccgac cgagatggtg
     7501 gtgatcgatc cgaacgccct ggtgccggga cgctacgtcg ccacggtcag ggtcggtgcg
     7561 gccggtgtgc ccgaaccgca cccgtcgttc ggcgggcagc catatgccct cgtcgcgacc
     7621 gggggtctgg tgcagcgcga cctgccgcac ctgggcgaag tcgacgacgg cgcggggtcg
     7681 gcggcggcgc cgctcgtcgc cggcttcgac gggacacagg tcgtcctcga ctggtcggcc
     7741 tcctgtctgc ccggggacta catcgtctat aaggggacgc tggcctcgct ttccggttcc
     7801 tcgacatcgc aggccacggt gctggccgcg tctaccggcg gcctgcccgg cgcaaccgac
     7861 gccgccctcg aagggaatca ttactatgtc gtcgcgccgc acgacggcgc cttcgagggt
     7921 tcctacggcc gcgactccga tctggccgag cggccgcaga gcgtgaactc gcccttcttg
     7981 cgttcgctcg agccttgctc cccgccctag agcccgcgtt acgagttcgc ccgcgccggc
     8041 ggccgggtcg gtcaggggct cgcgaccggc gcccggtagg cgtcggcctc ggcagcgcgt
     8101 cccagcgcgt cgtactcggc gaccaggtcc tcgcgggccg cacgcagcca gctcacggcg
     8161 ggatcggtct gccgcgtcag ggtctcgtga ccggcgagcg tttgcgcgag cgcctcttcg
     8221 tgccggccct ggcgtagcag cacgcgaccg agcttgatcc gcgcgatacc gacgttgagg
     8281 tgctcggccg acagcgtggc gacgtagatc tcgatcgcct cgcggaactg gggctcggct
     8341 tcggtccagt ggccgcgagc catgaagacg ctcgccaggt tcgacaccgc ggtgccgacg
     8401 agatagtgcc cctgcgggtg ggccgcgcgc tggatcgcga ccgtccggcg gaaggcggcc
     8461 tcggccgccg gatagtcgtc gcgctgcagc gcgatcgagc cgaggtcgtt cagcgcggat
     8521 gcgacccgcg ggtggtccgg accgtagacg cgctcttggg tcgcgagcgc ctcgctcagc
     8581 tcgcgctgcg cctcgtcgaa ccgctccgcg taaaccagcg agcgaccgag catgatctgc
     8641 gccgaagcgg tcgccggatg atcgtcgccg taccactccc ggttgatctc gagcgcgtcg
     8701 cggtagtgct gctcggcgcc agcgtaatcg ccctgctcgt gccggatagc cccgaggttg
     8761 accaggtctt ccgcgacgtg cgggtgccgg tcgccgtaga ggccgcgact gagcacgagc
     8821 acccgcccca tcaccgcctc ggcctcggcc aagcgcccgc ggtagaaatg agcgttggcc
     8881 agctcgaaca gcgtgtcggc cagctcgggt gtttcttcgc gatgccgctc gcggatcgcg
     8941 gccgccagct cgaggttcgc cgccgcatcg tcgtacgatc cccgctcgat cagcacgtga
     9001 ccgagggcgg tctgtgccgc ggcgatcgac gggtggtcgc gcggccgcgc ggcgaccagc
     9061 gtggcgagcc cctcgcggca cagcgtctcg gcctcgtcgt actgcgcctg gtccgagcgc
     9121 agcaggccga gagcgatccg gctctccgcc tcatcgatcc ggcggtcgcc gggcagctcg
     9181 cgccgacgct cgagtgatgc ctcgagcagc tcgtcggcgc ggtccagatc acccagcttg
     9241 tggtagatcc ggccgagcgt ctcgtacagc tcggcctgga tcaccggctc ggcggcgaga
     9301 ctgcgcgcct cgatcacgcc tcgctcgatc aacgtcacga cgcgcatctc ggcggccggt
     9361 cccgccgcct cgtcgccacc ctcgaacagg ttggtgagga agcgctggat gcgctgcgtt
     9421 cgcgccgcct cggcaacggc ctcgttgcgc gcgcgagcta ggcgcgtcgt gtagtaggtc
     9481 gccatgccga ccagcaccgc ggcgatgccg accgcggcga caacgctcac gcgatggcgg
     9541 cgaatgaact tgccgatgcg gtagccgacg acgccggggc gggcgagcag cggttccccg
     9601 gccaggtaac gcccgacgtc gccgatcagc gcgtcgaccg tcgggtaacg ccggagcgga
     9661 tcccggtgca tcgccgtcag gcacagcacg tcgaggtcgg accacgagcc atagcgcccc
     9721 ggccggaagc gtggccgctc gatcggcgac gcggtgaccc tcgcggcaac gaccgacggg
     9781 cgctcggggt cgtgatcgac caggcgcgag atcgcctcgg ccggcgtcag ccccgagagg
     9841 tcgaacggcg gtcgaccggt cagcagctcg tagaggatca cgccgagcga gtagatgtcg
     9901 ctgtggatcc cgacgcgctc gccgcgaagc tgctcgggcg aggcgtaagc cggcgtcatc
     9961 atccgcagcg cggtacgcgt ttgatcgatc ggctgatgct cgctcgcgag gtgcttggcg
    10021 atgccgaaat cgagcagctt caccgttccg tcggccttga ccaggatgtt cgacggcttg
    10081 agatctcgat ggatcaccgc ctggccgtgg gcgtattgca ccgcctcgca gaccgcgcgg
    10141 aacagctcga gccgtccctc gatcgaggtg ccgtgctcgc ggcagtagtc ggtcagcggc
    10201 actccctcga cgtactccat cacgaaccac ggcgtcccgt cgggtagcgc gtcggcgtga
    10261 tgcagctgag cgatcgacgg gtggttcagt tgcgcgagcg tccgctgctc ggcgcggaac
    10321 cgctcgcgcc gcgccgggga caaccaggcg tcgcgcagga tcttgatcgc ggcgacgctg
    10381 ccgaggtcgt cccgttccgc gcggtagacg acgcccatcc caccttcgcc gagcagaccg
    10441 ccgaggcggt acggtccgaa ggtcccgtgc ggaacgggcg agcccagcac ccgagcggcg
    10501 acgtgcgcga ggtcgcgatc gagcgccgag ccggcgcgcg cgtcttcctc gagcagtccc
    10561 tcgacctcgg cgacgagcgt cgggtcggcg gcgcgctcgg cgtcgaggat cgccgcgcgc
    10621 tcggcggccg gccggtcggc gacgcggtgg aacagggcct gcacccgctc ccagcgggcc
    10681 ggatcgatca cgaccgccgc ccgatctcgc gggcgagcca agcgcgggcc gcgcgccagt
    10741 cgcgcagcac ggtcgcctcg gacacgccga gcagcccggc cgtctccccg acctcgagac
    10801 cgccgaagta gcggctctcg acgatcgccg cctgacgcgg gttgacccga gccagcgccg
    10861 cgaggctggc gtcgagcgcc agcaggtcgt cggccacgtc gaccgcgccg aggctctcgt
    10921 cgaaggtgac gggtgccagt ccgccgccgc gcttgtcggt cttgcggcgt cgcgccgcct
    10981 cgaccagcac ctggcgcatc gcccgggcgg cgatccgctt gaagtgcagg cgcgagaccg
    11041 taccgacggg tggcgactgg gccagcttga accacgcctc gttgacgagt gccgtcgggt
    11101 tgagcgtcgc gctcgggtcg ccccgacgta ccgtcgtggc caaccgcctc agctcctcgt
    11161 aggtcgcgcc gaacagcagg tcgagcgtct cgcggtcgtg aaccgccgca tcgaacggat
    11221 cgtcgttcga ctcgtcaacc atcgcccacc cgccggccgg acgtacgccg ccggatcgtc
    11281 ccccgttggc cggaaagcta cgccactccg tacccgttcg gcaatcggat ttctcggagg
    11341 cgcccgctga cggtcggcgc cgagcgccga cgcaatgccg ggtgagttcg aatcaaccga
    11401 ggaggagcct ctcatgcaga tcgaaagaag cccgttcctg agtctcgacc gctcgcaggt
    11461 gatcggctac ttcaaggccg ccggttcccg cgaccccgac gtgctgcaca cgcacaaggc
    11521 gagcctgatc tcgctcgcca agttcccgaa gttggcgggc acctacctga tggtggtcgg
    11581 cggtctgtgc acgctgctga tcctgctggc gccgatcgga ctcccgctgc tcgggctcgg
    11641 ctggtggacg cggcggcgcg gcgtgcgcaa cctcgagacg gtcgagagcg gcttcaccga
    11701 gttcatgcgt ggcgcggcgg cgtaggctcc agggccagcc cggtgcctca ggccgcgtcc
    11761 ccgaggaatg cggccaggcc gttgagcatc atctggaccg cgagcgtgat cagcaccata
    11821 cccatcagcc gctcgacggc gatcaggccg cgccgaccca gcatgcgtct gagaccctgt
    11881 gcgaagtaga gcgtcgaggc cgtcacgagc caggcgagga cggtcgctgc gagccacacc
    11941 ggccagcgtt cgggctcccg attcatgatg aacatcaccg aggccagggc cgacggcccc
    12001 gcgacgtagg ggatggcgag cggtaccaca aacggctcgc tgtcggcctc ctcgtcgaga
    12061 tcgacccgcg cccgcggaaa gatcatccgc aacgcgatca ggaacaggat cacgcccccc
    12121 gcgacgccga gcgacggctc ggagatctgc agtagcgcga ggacgtgctc gccgacgaac
    12181 aggaacgcga cgagcgcggc gagtgcgatc agcagctcgc ggacgaggac gatccggcgg
    12241 cgcgccggct cgacgcggtc gagcgccacg atgaagaacg ggacgttccc gaacgggtcg
    12301 agcacgagga ccagcagcag g
//
LOCUS       DASPGN010000029         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_122419, whole genome shotgun sequence.
ACCESSION   DASPGN010000029 DASPGN010000000
VERSION     DASPGN010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122419"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VD788_00875"
     CDS             complement(<1..651)
                     /locus_tag="VD788_00875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV74843.1"
                     /translation="MVGLERSVLPLVGEEDFGLTSAAAILAFVVAFGAAKALTNLAAG
                     DLAERFGRKRLLVAGWVVALPVPLLIGLAPSWWFIVAANLLLGVNQGLAWSMTVVMKI
                     DLAGPARRGLALGFNEAAGYLGVAVTAFATGALAASYAPRTVVWVGAALIAVTGLLIS
                     VLAVRDTSAHVTLEQRRHGAHQPAHANLRSAFAHASLRDPILRACCQAGLVNNLNDA"
     gene            complement(729..1484)
                     /locus_tag="VD788_00880"
     CDS             complement(729..1484)
                     /locus_tag="VD788_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV74844.1"
                     /translation="MLLRPFLNDAGSCASYLFGCTSHAKLAVVDPHADFVEDYVAAAA
                     AIGAPIVAVFETHVQADHVSGLPGLVERTGATAYLPAGAGVAFDHVALEDGEVVELGN
                     TLVTAIATPGHAPAHHAYTVADRRRGSDEPWLVFSGDSLLIGDVGRPDLHVVGDARGQ
                     ARRLHASLRRLLELPDPVVLFPSHYGGSVCGRGLSGNPVSSIGFERSHNPLLALIDPE
                     AFADALLAEMPPRPVDQERIVAANRSGEVGALA"
     gene            complement(1496..2161)
                     /locus_tag="VD788_00885"
     CDS             complement(1496..2161)
                     /locus_tag="VD788_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXV74845.1"
                     /translation="MASGSAKVALFDALASVAQALGSGRRAEIVDVLAQGERSVEDLA
                     GEISQSVANTSQHLQVLARAGLVRTRREGNRVFYRLADDRVSELWAAVRDVAGRHVAE
                     VSVLADEYLGGRDGVEQLSAVELEQRLARGDVVVLDVRPEPEFLAGHIVGARSAPLSE
                     LASLAPKLPRRREIVAYCRGPYCVYADDAVRLLRARGLRARRLDVGFPEWRRAGLPVE
                     ITH"
BASE COUNT          399 a          813 c          779 g          293 t
ORIGIN      
        1 ggcgtcgttg aggttgttca caagccctgc ttgacagcag gcacgcagga tcgggtcgcg
       61 caggctcgcg tgcgcgaagg cgctgcggag gttggcatgt gcgggctggt gcgctccgtg
      121 gcgacgctgc tcgagcgtca cgtgcgcgga ggtgtcgcgt accgcgagca cgctgatcag
      181 caggccggtc accgcgatca gcgccgcgcc gacccagacg acggtgcgag gcgcgtagct
      241 ggcggccaac gcgcccgtcg cgaaggcggt caccgcgacg ccgagatagc cggctgcctc
      301 gttgaagccg agcgcaagcc cccgccgcgc cggaccggcg aggtcgatct tcatcacgac
      361 cgtcatcgac caggcaagcc cctgattgac accgagcaac aggttggcgg cgacgatgaa
      421 ccaccaggac ggggcgagac cgatcagcag cggcaccggt agcgcgacga cccatcctgc
      481 aaccaggagc ctcttgcggc cgaaccgctc ggccagatcg cctgcggcga ggttggtgag
      541 agcctttgcc gcgccgaacg cgacgacgaa cgcgaggatg gcggccgccg acgtgaggcc
      601 gaagtcctcc tcgccgacga gcggcaggac gcttcgctcg aggccgacca tgccgccgac
      661 aagcgcgttc agcgcgacga gcagcccgaa ctggcgggcg ttcgctcgca tgccgagtcg
      721 gaccgcggtc atgcgagggc gccgacctca ccggagcggt tggcggcgac gatgcgctcc
      781 tggtcgaccg gccgcggcgg catctcggcg agcaacgcgt cggcgaacgc ctccggatcg
      841 atcaatgcca gcagcggatt gtgggatcgc tcgaacccga tcgaggagac cgggttgccg
      901 gacagcccac ggccgcagac cgagccgccg taatggctcg ggaagagaac caccgggtcc
      961 ggcagttcga gcagccgccg cagactcgcg tgcaaccggc gtgcctggcc gcgcgcgtca
     1021 ccgacaacat gcaggtcggg gcgaccgacg tcgccgatca gcagcgagtc gccgctgaac
     1081 accagccacg gctcgtcgct gccgcgccgc cggtcggcga ccgtataggc gtggtgggct
     1141 ggtgcgtgtc caggtgttgc gatcgcggtc acgagcgtgt ttccgagctc gacgacctcg
     1201 ccgtcctcga gcgcgacgtg gtcgaaggca acgcccgcac cggccggcag gtaggcggtg
     1261 gcgcccgttc gctccaccag cccgggcaga ccggacacgt ggtcggcctg gacgtgcgtc
     1321 tcgaacacgg cgacgatcgg cgcgccgatc gccgcagcag cggcgacgta gtcctcgacg
     1381 aagtcggcgt gcgggtcgac gaccgcaagc ttcgcatggg aggtgcagcc gaacagatag
     1441 ctggcgcagg agccggcgtc gttcagaaaa ggtctcagca gcatgtggtc tcctctcagt
     1501 gggttatctc gacgggcagg ccggcacgcc gccactccgg aaagccgacg tccaggcggc
     1561 gcgccctgag gccgcgcgct cgcagcagac ggacggcatc gtcggcgtac acgcagtacg
     1621 ggccccggca gtacgcgacg atctcgcgcc gccgcggcag cttgggcgcc agtgaagcga
     1681 gctccgacag cggcgccgaa cgcgcaccga cgatgtgccc cgcgaggaac tcgggctcgg
     1741 gacgcacatc gagcacgacc acgtcgccgc gcgccagccg ctgctcgagc tcgacggccg
     1801 agagctgctc gacgccgtcc cgcccgccca gatactcgtc ggcgagaacg ctcacctcgg
     1861 ccacatggcg acccgcgaca tcgcggaccg ccgcccacag ctcgctaacg cgatcgtcag
     1921 cgagacggta gaagaccctg tttccctcac gccgcgtccg aaccagaccc gctcgtgcaa
     1981 gcacctggag atgctgcgac gtgttcgcga cgctctgcga gatctcgccc gcgaggtcct
     2041 cgaccgagcg ctcgccctgc gccagcacat cgacgatctc cgcccgccga ccactcccga
     2101 gcgcctgcgc gaccgacgcc agcgcatcga acaacgcgac cttcgccgaa ccgctcgcca
     2161 catactcaag ttatcacttg agcacttgag aatcaaccga tgcgggtttt ccacccttcg
     2221 ggatacggcc gttccccaat gccgcgacaa cggacgccgg acacgatcac agcgatgtga
     2281 agac
//
LOCUS       DASPGN010000030         9450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_5795, whole genome shotgun sequence.
ACCESSION   DASPGN010000030 DASPGN010000000
VERSION     DASPGN010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9450
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5795"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="VD788_00890"
     CDS             <1..1242
                     /locus_tag="VD788_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXV74846.1"
                     /translation="ENADFAQACADAGVVFVGPPPAAMRAVGDKVRARQLMIDAGVPV
                     VPGTPPLGDDLPTIVAQAAGIGYPVLLKAAAGGGGKGMRVVHRADQLASMLALARGEA
                     RSAFGDDTVFIEKFVERPRHIEVQILADSHGDVVWLGERECSIQRRHQKLIEESPSPA
                     VDGATRARLGQLAVRAVRAAGYVNAGTVEFLRAADGSFYFMEVNARLQVEHPVTEEVT
                     GIDLVRSMLDIAAGGRLPIAQDELRIDGHAIECRIIAEDPSRNFTPSPGTIRGLRPPG
                     GLGVRYDDGTYPGYTVPVFYDPMIGKLICWGRQRREAIARMARALDELRIDGLTTSVS
                     FHRKVMEHPAFRAGELHTAFLDEHPELLSPHDDPWLNEIAVVAAAVSHFRGIEARSAR
                     GGGSADHGSAWKRHGRRGWRR"
     gene            1239..1730
                     /locus_tag="VD788_00895"
     CDS             1239..1730
                     /locus_tag="VD788_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXV74847.1"
                     /translation="MKITAKAGQQVLEVEIERSNGRYVVEVDGVRHLVDAHKLEADFY
                     SLLTGGRSYEVSVEVRGEAYHVRHGAAEQRVTLTDPSRRAREGGPSAHGPEKVVSMMP
                     GKVVRVLVAPGERVAAGQALMVVEAMKMENEISSTRDGTVKSVDVERGQRVEGGALLA
                     VIE"
     gene            complement(1746..2021)
                     /locus_tag="VD788_00900"
     CDS             complement(1746..2021)
                     /locus_tag="VD788_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXV74848.1"
                     /translation="MNKATLIEKIAKDTGITKVAAASAIESMIDGVSGALAKDQRVTL
                     VGFGSFGVSRRRARAGRNPQTGETIKIRAKKSVRFKPGKQLETVLNR"
     gene            complement(2108..2497)
                     /locus_tag="VD788_00905"
     CDS             complement(2108..2497)
                     /locus_tag="VD788_00905"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXV74849.1"
                     /translation="MGESGFDRRRHPRVETRIQVNGSPEDGAVVARMVTDNLSLAGLQ
                     CTSSADFPEMTRLAVRLMLPPIRGGGNEVEPVDVEAVVVRRTDLAASSGDARYRLALF
                     FTRIDDRSKERLVSFLEARKRGAVVSS"
     gene            2544..3347
                     /gene="larB"
                     /locus_tag="VD788_00910"
     CDS             2544..3347
                     /gene="larB"
                     /locus_tag="VD788_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HXV74850.1"
                     /translation="MERDALEKMLRRVRAGRLSVRQALDELATFPIADLGFATVDTHR
                     ALRRGFPEVVYGPGKTAEQIAAIVRSIDACGQTALVTRVDPEIAREVIERQPELRYLE
                     LPRALVLERRRRRGGRPGVVVVTAGTSDLPVAEEAALTAELMGERVERIRDVGVAGLH
                     RLTRHRATLAAARVIVAVAGMEGALPSVVAGLTDCPVIGVPTSTGYGAGAGGQAALLA
                     MLNSCASGLTVVNIDNGFGAGFAASLINRRSKIKRKNTPTRHTRKNNTP"
     gene            3546..3752
                     /gene="rpsU"
                     /locus_tag="VD788_00915"
     CDS             3546..3752
                     /gene="rpsU"
                     /locus_tag="VD788_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXV74851.1"
                     /translation="MPLIKVREDESLENALKRFKRKCEKSGILTEIKKRQHYEKPSVK
                     RKRKALAARKKLLKRLAQERRLNG"
     gene            3823..5610
                     /gene="dnaG"
                     /locus_tag="VD788_00920"
     CDS             3823..5610
                     /gene="dnaG"
                     /locus_tag="VD788_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HXV74852.1"
                     /translation="MGGHWVGGHFPKTVIEAVRDAADIVQVVSEYVPLKRAGSRWKGI
                     CPFHQEKTPSFSVDPTNQLFYCFGCGTGGDLFKFVMLYDKLDFPEVVETIARRFGVPL
                     PEPDPRVSRTTDKRERLFAINRDSADFYAGLLRGDAGARCRAYLERRGLDQPTIERLG
                     LGYAPDSWDALARQLAARRYGEDELLEAGLVVQGKSGRGVYDRFRDRLIFPIRDVRGR
                     TVAFGGRAIGDAEPKYINSPETPTYTKGDQLYGLDLARDAIRREGFAVVVEGYLDLAA
                     LLQAGIDQVVASLGTAFTAAQSRLLARYTDRVLVSYDGDAAGAEATTRSLDLLLEKGF
                     EVRVVELPAGKDPDDFVRDEGPGAYERLLRQAPEYLEFLIRRQQASRDLSRPQEKVAA
                     VNAVLPHLAKLGTSIERSEWAGRLADTLQVDDELVLAELRGAARAARASVRQPVRDRP
                     SPRWSEGLLVRLLLENDEQRARFAERTDLDDDIESMTVAQIVKTIRRLIDDGTEVSYR
                     TVLQSLSDEADRELLTGIAFRDDPVEGTIDDCLWVSERDRLRRLQREAVRRLGELEGE
                     SPAGDSAREDVDEMLARVQEIARQRDALY"
     gene            5641..7332
                     /gene="rpoD"
                     /locus_tag="VD788_00925"
     CDS             5641..7332
                     /gene="rpoD"
                     /locus_tag="VD788_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXV74853.1"
                     /translation="MGKKRSDNASLGVEEKYEEVRQLVNMGRERGFLALDEINELLPD
                     DVSTSTDEIEEVFAVFEAHGIEVVDAETKEQLKVTVSTDPKSKTDVPPALLEKTNDPV
                     RMYLREMGTVPLLTRQGEVSIARRIERGERRVVNALARSDYVVREIQALGELIRAGHA
                     SPNWFSDGDDGGDDKAPQKRLAHARRTIVKINKHVDEIGSQQKRLRRLKPGGKTYREA
                     LRGIARERVRVAQQLRQMRLVPQQIDTLARAIVDSSQKVRRHERSIQDLTLRMKSYRD
                     QNLRREVRRKIEQLRADIRTIVSDLGVDRRSLNLEASLILRGAREAKRAKSELVEANL
                     RLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMIETINKLIRTQRGLVQELGREPSPDEIAQKMGIPVAKVRKVLKI
                     AQEPISLETPIGEEEDSHLKDFIEDRGAISPVDAVMHVNLRDQTQSVLKTLTPREEKV
                     LKMRFGVGGGSEHTLEEVGQNFTVTRERIRQIESKALRKLRHPSRSKALRIFMGE"
     gene            7356..8063
                     /locus_tag="VD788_00930"
     CDS             7356..8063
                     /locus_tag="VD788_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXV74854.1"
                     /translation="MANDDRFRESTRDRLRRLRLFGELEDEALDRLAGLSRILHLERK
                     QNLFAEGEPYRGIFVVLDGLVVVYKLSTDGRMLILEVCRPGDAFAWGPLFEQEDAGYP
                     ASARATRASEILFLPKQAFTPFLKRHPEVAWELLKDSAARMKELNLRLEGVTLREVSS
                     RVAQYLLRELEAGGVAGDAQPELELPLTKGSIASYLGTVHETLSRTFAKLIRDKVIAV
                     HGSRVKILDPKKLERLL"
     gene            complement(8064..8432)
                     /locus_tag="VD788_00935"
     CDS             complement(8064..8432)
                     /locus_tag="VD788_00935"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HXV74855.1"
                     /translation="MGWLATGAIVAGSAVALGAFGAHGLKTRVAAELLAVFETGVRYQ
                     MYHGLGLLAVGLAAARWGGAAIQLAGWLFLAGVALFSGTLYLMTWTGARWLGAVTPIG
                     GLLLIAGWIVLAVGALRAGG"
     gene            complement(8450..>9450)
                     /locus_tag="VD788_00940"
     CDS             complement(8450..>9450)
                     /locus_tag="VD788_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXV74856.1"
                     /translation="RPVEQVARAALPVTMHALWLERRRLRLRDDLERPDGGGGEAVVR
                     VRCAGVCGTDLELERGYYPFTGVPGHEFVGIVDRGPDGWVGRRVVGEINASCGACAVC
                     VAGRANHCGRRTVLGIVGRNGAFAEYLSLPVANLHAVPDDLPDDVAVFTEPLAAALRI
                     GQQLRIGAEHEVLVVGDGKLGQLVARGLARTGCRLFVVGRHDGKLALLERAGIRGGRA
                     DAVVAGGYDVAVECTGNPDGFAIARDALRPAGTLVLKSTYAGRLTLDASSLVVDEITL
                     VGSRCGPFAPALRLLAGGEIDPRPLIHGRYPLERAIEAFEHAARPGVLKILIDVER"
BASE COUNT         1609 a         3190 c         3291 g         1360 t
ORIGIN      
        1 gagaacgccg acttcgcgca ggcctgcgcc gacgcaggcg tcgtcttcgt cggtccgccg
       61 ccggcggcga tgcgcgcggt cggcgacaag gtgcgggccc ggcagctgat gatcgacgcc
      121 ggcgtgccgg tcgttcccgg caccccgccg ctcggcgacg acctgccgac gatcgtggcc
      181 caggccgccg gcatcggcta cccggtgttg ctcaaggccg ccgccggcgg cggcggcaag
      241 ggcatgcgcg tcgtccaccg cgccgaccag ctggcctcga tgctggcgtt ggcccgcggc
      301 gaggcgcggt cggccttcgg tgacgacacc gtcttcatcg aaaagttcgt cgaacggccg
      361 cgtcacatcg aggtgcagat cctggccgac tcgcacggcg acgtcgtctg gctcggcgag
      421 cgcgagtgct cgatccagcg gcgccaccag aagctgatcg aggagtcgcc ctcgccggcg
      481 gtcgatggcg cgacccgggc tcgcctcggc cagctggcgg tgcgcgcggt gcgcgccgcg
      541 ggctacgtca acgcgggcac cgtcgagttc ctgcgcgccg cggacggctc gttctacttc
      601 atggaagtca acgcgcggct acaggtcgag caccccgtca ccgaggaggt gacgggcatc
      661 gacctcgtgc gcagcatgct cgacatcgcg gcgggcggcc ggctgccgat cgcccaagac
      721 gagctgcgga tcgacggcca cgcgatcgag tgccggatca tcgccgagga cccgtcgcgc
      781 aacttcacgc cgtcgccggg gacgatccgc gggctcagac ctcccggcgg actcggcgtg
      841 cgctacgacg acgggaccta tccgggctac accgtgccgg tgttctacga cccgatgatc
      901 ggcaagctga tctgctgggg gcgccaacgc cgggaggcga tcgcgcgcat ggcgcgcgcg
      961 ctcgacgagc tgcgcatcga cgggttgacg acctcggtca gcttccatcg caaggtgatg
     1021 gagcaccccg cgttccgcgc cggcgagctg cacaccgcat tcctcgacga gcatcccgag
     1081 ctgctgtcgc cgcacgacga tccgtggctc aacgagatcg cggtcgtcgc ggcggcggtc
     1141 agtcattttc gcgggatcga ggcgcgctcg gctcgtggcg gcggctccgc cgaccacggc
     1201 tcggcctgga agcgccacgg gcggcgcggg tggcggcgat gaagatcacg gccaaggccg
     1261 gccagcaggt cctcgaggtg gagatcgaac gatcgaacgg ccgctacgtc gtcgaggtcg
     1321 acggtgtacg ccatctcgtc gacgcgcaca agctcgaggc cgacttctac tcgctgctga
     1381 ccggcggacg ctcctacgag gtgtcggtcg aggtccgcgg cgaggcctac cacgtgcgcc
     1441 acggcgcagc cgagcaacgg gtcacgctga ccgatccgag ccgtcgcgcg cgcgagggcg
     1501 gaccgtccgc gcacgggccg gagaaggtcg tctcgatgat gccgggcaag gtcgttcgag
     1561 tgctcgtcgc gcccggcgag cgggtcgcgg ccggtcaggc gttgatggtg gtcgaggcga
     1621 tgaagatgga aaacgagatc tcgtcgacgc gagacggcac ggtcaagagc gtcgacgtcg
     1681 agcgcgggca acgcgtcgag ggtggcgcgc tgctggcggt catcgagtga tggtccgggc
     1741 gccggtcagc ggttgagcac cgtttccagc tgtttgcccg gtttgaagcg aacggacttt
     1801 ttcgctcgga tcttgatcgt ttccccggtc tgaggattgc ggccggcgcg ggccctgcgc
     1861 cggctgacgc cgaagctgcc gaacccgacc agcgtcactc gctggtcctt ggccaacgcg
     1921 ccgctgacac cgtcgatcat gctctcgatc gcggacgccg ccgcgacctt ggtgataccc
     1981 gtgtccttcg cgatcttctc gatcaatgtc gccttgttca tcccatcacc tcccttcgca
     2041 tcgtgcgggg ccatgaacga tcgatcacgg cggtcgcgcg attgtaatcg cgcacgggag
     2101 agaggtgtca agacgagacg acggctccgc gctttcgcgc ctcgaggaag ctgaccagac
     2161 gctccttcga gcggtcgtcg atccgggtga aaaacaacgc cagccggtag cgcgcatcgc
     2221 cgctcgacgc ggccaggtcg gtgcggcgca cgacgacggc ctcgacgtcg accggttcga
     2281 cctcgttgcc cccgccgcgg atcggtggca acatcaaccg aacggcgagg cgggtcatct
     2341 cggggaaatc ggcgctcgac gtacactgca acccggcaag cgacagattg tcggtcacca
     2401 tgcgcgcgac gaccgctccg tcctccggac tgccgttgac ctggatccgg gtttcgactc
     2461 tgggatgccg acgacgatcg aagcctgatt cgcccaagat gccacctccg aggggcgcat
     2521 cctagcacga cggagaaagg ccgatggaac gcgatgcgct cgagaaaatg ctgcggcggg
     2581 ttcgcgcggg acggctgtcg gtccggcagg cgctcgacga gctggccacc tttccgatcg
     2641 ccgatctcgg cttcgccacg gtcgacacgc accgtgcgct gagacgcggc ttcccggagg
     2701 tggtctacgg cccgggcaag accgccgagc agatcgccgc gatcgtgcgc tcgatcgacg
     2761 cctgtggtca gacggcgctc gtcacccgcg tcgacccgga gatcgcgcgc gaggtgatcg
     2821 agcggcagcc cgagctgcgc tacctcgaac tgccgcgcgc gctggtgctc gagcggcgcc
     2881 gtcggcgcgg cggacgcccc ggagtggtcg tagtcaccgc cggcacgtcg gacctgcccg
     2941 tcgccgagga agccgcgctg accgccgagc tgatggggga gcgggtcgag cggatccgcg
     3001 acgtcggcgt ggccgggctc caccgcctga cgcggcaccg cgccacgctc gccgcggcgc
     3061 gggtgatcgt ggccgtcgcc gggatggaag gggctctgcc gtcggtcgtg gccgggctga
     3121 ccgactgccc ggtcatcggt gtcccgacga gcaccggcta cggcgccggg gccggcgggc
     3181 aggcggcgct gctcgcgatg ctcaactcgt gcgcgtcggg gctgaccgtc gtcaacatcg
     3241 acaacggctt cggcgccggt ttcgccgcct cgttgatcaa ccgacggtcg aaaataaagc
     3301 gaaaaaacac cccgacccgg cacacgcgca agaacaacac cccctaattc ttccaattgc
     3361 cttgccttcg ccatatttct gtcgtaaaag tagacaacga catcgcacta cgccgagaat
     3421 gcttcaaaaa gcggcgagaa tttgactagc tggggtcccg ttgctaacat tttccgactt
     3481 gtccgggacg aattatttga agcccgacac gcccgacacc atcaggaaag cggggtgatg
     3541 gaacgatgcc gctgatcaag gtgcgcgagg acgagagcct cgagaacgcc ttgaaacgct
     3601 tcaagcggaa gtgcgaaaag tctgggatct tgaccgagat caagaagaga cagcactacg
     3661 agaagccgag cgtcaagcgc aagcgaaagg cgctggccgc tcggaagaaa ctgctgaagc
     3721 gtctggcgca ggagcgcagg ctgaacggtt gatcgttgtc atcgccccgg ggaccgtcga
     3781 gcggcgtcct gccacctcgc ctgtcacccg gggctcgttc ggatgggtgg tcattgggtg
     3841 ggaggccact ttcccaagac ggtgatcgag gcggtccgcg acgcggcgga catcgtccag
     3901 gtcgtctcgg aatacgtccc gctcaagcgg gccggttcgc ggtggaaggg aatctgcccg
     3961 ttccaccagg agaagacacc ctccttttcg gtcgatccga ccaaccagct gttctactgt
     4021 ttcggctgcg gcacgggtgg cgatctgttc aagttcgtca tgctgtacga caagctcgac
     4081 ttccccgagg tcgtcgagac gatcgcccgg cgcttcggcg tgccgctgcc cgagcccgac
     4141 ccgcgcgtct cccgcaccac cgacaagcgc gagcgcctgt tcgcgatcaa ccgcgattcc
     4201 gccgacttct acgccggtct gctgcgcggc gatgccggtg cccgctgccg cgcctatctc
     4261 gaacgacgcg gcctcgacca gccgacgatc gagcgtctcg gtctgggcta cgcacccgat
     4321 tcgtgggacg cgctcgcgcg gcagctcgcc gcgcggcgtt acggcgagga cgagctgctc
     4381 gaggcgggcc tcgtcgtgca aggcaagagc ggtcgtggcg tctacgaccg cttccgcgac
     4441 cggctgatct tcccgatccg cgacgttcgc gggaggacgg tcgcgttcgg cgggcgcgcg
     4501 atcggcgacg ccgagcccaa gtacatcaac tctcccgaga ctccgacgta taccaaaggg
     4561 gaccagctgt acggcttgga cctggcgcgc gacgcgatcc gtcgcgaggg cttcgccgtc
     4621 gtcgtcgagg gctatctcga tctggccgcg ctgctgcagg ccggcatcga tcaggtcgtc
     4681 gcgtcgctcg gcaccgcgtt cacggcggct cagtccaggt tgctcgcgag gtacacggac
     4741 cgcgtgctgg tctcctacga cggggatgcc gcgggggccg aggcgacgac gcgctcgctc
     4801 gacctgttgc tcgagaaagg gttcgaggtt cgcgtggtag agctgcccgc cggcaaggac
     4861 cccgacgatt tcgtccggga cgagggtccg ggggcctacg agcggctgct gcgccaggcg
     4921 cccgagtacc tcgagttcct catccgccgc cagcaggcct cccgcgatct gagccgcccg
     4981 caggaaaaag tcgcggcggt caacgcggtg ttgccgcatc tggccaagct cggcacgtcg
     5041 atcgaacggt cggagtgggc cggtcggctg gccgatacgc tgcaggtcga cgacgagttg
     5101 gtgctggcgg agcttcgcgg cgcggcgcgc gcggcgcgcg ccagcgtcag gcaaccggtc
     5161 cgcgaccggc cgtcgccgcg gtggagcgag ggcctgctgg tgcggctgct gctcgagaac
     5221 gacgagcagc gcgctcgctt cgccgagcgc accgatctcg acgacgacat cgagtcgatg
     5281 accgtcgcgc agatcgtcaa gacgatccgt cgtctgatcg acgacgggac cgaggtgtcc
     5341 taccgcacgg tgctgcaatc gttgtccgac gaggcggacc gcgagctgct caccggcatc
     5401 gcgttccgcg acgacccggt cgagggaacg atcgacgact gcctgtgggt ctccgagcgg
     5461 gatcgcctcc ggcgattgca acgggaagcg gtccgccgac tgggagagct cgagggcgag
     5521 tcgccggccg gggattccgc gcgcgaagac gtcgatgaaa tgctggcccg agtgcaagag
     5581 atcgcaagac agcgcgacgc actttactga tccgatcatc gtgggagcga tacggaaacg
     5641 atgggcaaaa aacgaagcga caacgccagt ttgggcgtcg aagaaaagta cgaagaggtc
     5701 cgccaactgg tcaacatggg ccgcgagcgc ggcttcctcg cgctggacga gatcaacgag
     5761 ctgctgcccg acgacgtcag cacgtcgacc gacgagatcg aagaggtgtt cgccgtcttc
     5821 gaggcgcacg gcatcgaggt ggtcgacgcc gaaacgaaag aacagctgaa ggtcacggtc
     5881 agcaccgacc cgaaatcgaa gaccgacgtg ccgccggcgt tgctcgagaa gacgaacgac
     5941 cccgtccgca tgtacctgcg cgagatggga accgtgccgt tgctgacccg ccagggcgaa
     6001 gtgtcgatcg cccgccgcat cgagcgcggc gagcgtcgcg tggtcaacgc gctggcccgc
     6061 agcgactacg tcgtccgcga gatccaggcg ctcggcgagc tgatccgcgc cggccacgcc
     6121 tcgcccaact ggttctcgga cggcgacgac ggcggcgacg acaaggcacc gcagaagcga
     6181 ctggcccacg cgcgccgcac gatcgtcaag atcaacaagc acgtcgacga gatcggttcg
     6241 cagcagaagc ggctgcgacg gctcaagccc ggcggcaaga cctatcgcga ggcgctgcgc
     6301 gggatcgcgc gcgagcgcgt ccgcgtcgcg cagcagctgc ggcagatgcg gctcgtgccg
     6361 caacagatcg acacgctggc ccgcgcgatc gtcgattcga gccaaaaggt gcgccggcac
     6421 gagcgcagca tccaggacct gaccctgcgc atgaagagct atcgcgatca gaacctgaga
     6481 cgcgaggtgc ggcgcaagat cgagcagctt cgcgcggaca tccgcacgat cgtcagcgat
     6541 ctgggggtcg accgccgctc gctcaacctc gaggcgtcgc tgatcctgcg cggcgcgcgc
     6601 gaggcgaagc gggccaagag cgagctggtc gaggccaatc tgcgtctggt cgtctcgatc
     6661 gccaagaagt acaccaaccg cgggctgcag ttcctcgacc tgatccagga aggcaacatc
     6721 ggcctgatga aggcggtcga caagttcgag taccgccgcg gctacaagtt ctcgacctac
     6781 gccacctggt ggatccgcca ggcgatcacc cgcgcgatcg ccgaccaggc gcgcacgatc
     6841 cgcattccgg tgcacatgat cgaaacgatc aacaagctga tccgcacgca gcgcgggctg
     6901 gtgcaagagc tgggtcgcga gccgagcccc gacgagatcg cgcaaaagat gggcatcccg
     6961 gtggccaagg tgcgcaaggt gctgaagatc gcgcaagagc cgatctcgct cgaaacgccg
     7021 atcggcgagg aagaggacag ccacttgaaa gacttcatcg aagaccgggg agcgatttcg
     7081 ccggtcgatg cggtgatgca cgtcaacctg cgcgatcaga cccagtcggt gctgaagacg
     7141 ctgacgccgc gcgaggagaa ggtgctcaag atgcgtttcg gcgtgggcgg cggcagcgag
     7201 cacacgttgg aggaggtcgg gcagaacttc accgtgaccc gcgagcgaat ccgtcagatc
     7261 gaatcgaagg cgttgcgcaa actgcggcac ccgtcgcgca gcaaggcgtt gcgtatcttc
     7321 atgggcgagt agcgcgggcg gtacggcgga ccgcgttggc aaacgacgat cggttccgcg
     7381 aaagcacccg cgaccggctc aggcggttgc ggctgttcgg cgagttggag gacgaggcgc
     7441 tcgatcggct ggccgggctg tcgcggatcc tgcacctcga acgcaagcag aatctgttcg
     7501 cagagggcga gccgtatcgc ggcatcttcg tcgtgctcga cggattggtc gtcgtctaca
     7561 agctgtcgac cgacggccgg atgctgatcc tcgaagtctg tcgcccggga gatgcgttcg
     7621 cctggggacc gttgttcgag caggaggacg cgggctaccc ggccagcgcc cgcgccacgc
     7681 gcgcgagcga gatcctgttc ctgccgaagc aggccttcac cccgttcctc aagcggcacc
     7741 cggaggtcgc ctgggagctg ctcaaggact cggcggcgcg gatgaaggag ctgaacctgc
     7801 ggctcgaggg cgtgacgctg cgcgaggtca gctcgcgcgt ggcccagtac ctgctgcgcg
     7861 agctcgaggc gggcggggtg gccggcgacg cacagcccga gctcgagctg ccgttgacca
     7921 aggggtcgat cgcgagctac ctcggcacgg tgcacgagac gctgagccgg accttcgcca
     7981 agctgatccg cgacaaggtg atcgcggtcc acggcagccg ggtcaagatc ctcgacccga
     8041 aaaagctcga acggctgctc tgatcagccg ccggcgcgca gcgcgccgac cgcgagcacg
     8101 atccagccgg cgatcagcag cagcccgccg atcggagtga cggcgcccag ccagcgcgcc
     8161 ccggtccagg tcatcaggta gagcgtgccc gagaagagcg cgacacccgc gagaaacagc
     8221 cagccggcga gctggatcgc ggcgccgccc caacgcgcgg cggccaaacc gacggccagc
     8281 aggccgaggc cgtggtacat ctgatagcgc acgcccgtct cgaagacggc gagcagctcc
     8341 gcggcgacgc gggtcttcag cccgtgcgcg ccgaacgccc ccagcgccac cgccgacccg
     8401 gcgacgatcg cgcccgttgc caaccacccc atcgttccct ccgcacgcgc tagcgctcga
     8461 cgtcgatcag gatcttgagc acgccgggcc gcgccgcgtg ctcgaacgcc tcgatcgccc
     8521 gttcgagcgg gtagcgaccg tggatcaacg gccgcgggtc gatctcaccg ccggccagca
     8581 gacgcagcgc gggggcgaac ggaccgcagc gcgagccgac gagcgtgatc tcgtcgacca
     8641 cgagcgacga cgcgtcgagc gtcagccggc cggcgtaggt gctcttgagt accagcgtac
     8701 cggccggccg cagcgcgtcg cgcgcgatcg cgaagccgtc cgggttgccg gtgcactcga
     8761 ccgcgacgtc gtacccgccg gcgacgacgg cgtcggcccg gccgccacgg atccccgccc
     8821 gctcgagcaa cgccagcttg ccgtcgtgac gcccgacgac gaacagccgg cagcccgttc
     8881 gggccagtcc gcgagcgacg agctggccga gcttgccgtc gccgacgacg agcacttcgt
     8941 gctccgctcc gatccggagc tgctggccga tacggagcgc cgcggccaac ggctcggtga
     9001 agacggcgac gtcgtcgggt aggtcgtcgg gcaccgcgtg caggttggcg acggggagcg
     9061 acaggtactc ggcgaatgct ccgttgcgac cgacgatgcc gagcacggtg cggcgcccgc
     9121 agtgattggc gcgccccgcg acgcagacgg cgcacgctcc gcagctcgcg ttgatctcgc
     9181 cgacgacgcg ccggccgacc caaccgtccg gcccccgatc gacgatgccg acgaactcgt
     9241 gaccggggac gccggtgaac gggtagtagc cgcgctcgag ctcgagatcg gtgccgcaca
     9301 cgccggcgca gcggacgcgc acgaccgctt caccgccgcc gccgtccggc cgctcgagat
     9361 cgtcacgcaa gcggagcctg cgccgctcga gccacaaggc gtgcatcgtg acggggagcg
     9421 ccgctcgcgc tacctgctcg acgggtcggg
//
LOCUS       DASPGN010000031         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_708464, whole genome shotgun sequence.
ACCESSION   DASPGN010000031 DASPGN010000000
VERSION     DASPGN010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708464"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VD788_00945"
     CDS             <1..462
                     /locus_tag="VD788_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74857.1"
                     /translation="VTRDASGRPTAARLLRSWRETVKIAGRDESRRVEIVYDYAAGVA
                     RRRSYAADGALIADQPLAAQPQPSPEEVSDAVALLRADADLGVLAERVGAVFDGGFTL
                     EEGEGKPCGPGARCLQIFMLSQSRFALHRRTAVDVRAERIAHRSYQPPSGE"
     gene            1393..2214
                     /locus_tag="VD788_00950"
     CDS             1393..2214
                     /locus_tag="VD788_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HXV74858.1"
                     /translation="MAAYADGESVADEDLVFWYRSSAAHAGGTIEHCEHTGPLLVPIG
                     DWLDPDGDKRVNPDDNCPFLANTSQFDADLDGVGDDCDNCVATQNADQRDLDEDGFGD
                     ACDDDRDGDGVLDASDNCPDDSNADQRDFDLDSLGDPCDPDRDGDGLPNLVDNCPDDP
                     SADQLDLDLDGLGDICDPDDDGDGLLDSVETGTGIYVSPEDTGTDPRRADTDGDGVSD
                     GDEVNGEIFTDPNDPDDNPTAIPAVSGWGMLVLGALLLAVAAAFRLRARRLRSTR"
     gene            complement(2336..>3400)
                     /locus_tag="VD788_00955"
     CDS             complement(2336..>3400)
                     /locus_tag="VD788_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HXV74859.1"
                     /translation="LAMGYTPASWIVGSGVALGLGISFVVVTRGVHWLNVSNAIVAPG
                     LAALIAFMLYMLIAEHGWREILAAEPLRPFPNPIVNYVVAVEIGIAGGIGWWGGLGFL
                     ARNTRTRRNAVYPVLLQMGLFQALACAVALFSALVVQSDDPTEWMVPLGGPVLGVLAL
                     CFVALANVTSSSVSMFASGLALRHIERFRLMPWSTLMQLTVVPCLPFIVWPQQLYDLG
                     DAFLTYNGTLFAPVAGILFTDYFLLRRQKLDLRSIFSDDPSAAYHYARGYNWVALASV
                     VLGQAVYLSLYNPLTSEVTEIFRFLPASIASFALPAASYAIGVRTLRARTGRSDAPAR
                     APAARDPQSTSIRAISMPNI"
BASE COUNT          580 a         1174 c         1186 g          460 t
ORIGIN      
        1 gtgacgcgcg acgcgagcgg acggcccaca gcggcgcgac tgctgcgcag ctggcgcgag
       61 accgtgaaga tcgccggccg cgacgagtcg cgccgggtcg agatcgtcta cgactacgcg
      121 gcgggcgtcg cgcggcggag aagctatgcg gccgatggcg cgctgatcgc cgatcaaccg
      181 ctcgccgcac agccgcagcc gtcaccggag gaagtgtcgg acgcggtcgc gctgctgcgc
      241 gcggatgccg acctcggcgt tctcgcggag cgcgtcggcg cggtcttcga cggcggcttc
      301 acgctcgagg aaggcgaggg caagccgtgc ggccccggcg cacgctgcct gcagatcttc
      361 atgctctcgc agtcgcgctt cgcgctgcat cggcgcacgg cggtcgacgt gcgcgcggag
      421 cgcatcgcgc accgcagcta ccagccccct tcgggcgaat gagctgacgt gaccgccgcc
      481 tcccgcgcgc tcgccgtgct cgccattgcg gcaggcgcgc tcgtcgctgc gcgccccgcg
      541 agcgcggggc agccgagctg cacgggcgac gagcagctga tgcagtggcc caccaacgat
      601 ccgctctggg agatgtgctg gctgcgcccc gcgctcagca gcggcggcaa cggctcgggg
      661 ctcgagctgc gcgacgtcta ctaccgcggc cagctggtgc tcaagcgcgc gcacgcgccg
      721 atcctctccg tcaagtatct ccgcggcggc tgcggctgct tccgcgactg gagcttcgag
      781 gaggcgccgt tcgtcgccga caacgtgctc tcccctggat acgccgagcc gacgtcgccg
      841 cccgtgacgg tctgcgaggc gggcggaggc ggcggcgacg tcggcagctt cacgggcgtc
      901 gccgccgaga agagcgccag ctcgctcatc ctgacgacgc agatgcaggc cggctggtac
      961 cgctacaaga tgagctggac gttcttcgcc gacggccgga tcatgccgtt cttcggcttc
     1021 gcggcggtcg atgcattctg catcaccttc tcccacgacc accacaacta ctggcgcctc
     1081 gacttcgaca tccagggagc ggaccgcgac agcgtggtcg agatcaacgc aggagccgcg
     1141 ccgctcgcga tcgaaaccga gaccacgcgc aactggatcg acgcgagcac cgcctgggag
     1201 atccgcgatt cgcggacgaa cctcggctac cgcctgctgc cgggcggcgc tccggactcc
     1261 gtagatctgg agctgcccgt cgacgagttc tcggaggccg atgcctggat cctgcgctac
     1321 aagcccggcg ccgagctcga ggacctcgac atcccgcccg acagcacgga gtactcgtac
     1381 tgccgcaatc gcatggccgc ctacgcggat ggcgagagcg ttgcggacga ggatctggtc
     1441 ttctggtacc gcagcagcgc ggcccacgca ggcggaacga tcgagcactg cgaacacacg
     1501 ggcccgctgc tggtgccgat cggcgactgg ctcgatccgg acggcgacaa gcgcgtgaat
     1561 ccggacgaca actgcccgtt cctcgcgaac accagtcagt tcgacgcgga tctcgacggg
     1621 gtcggagacg actgcgacaa ctgcgtggcc acccagaacg cggaccagcg cgatctcgac
     1681 gaggacgggt tcggcgatgc gtgcgacgac gaccgcgacg gcgacggcgt cctcgacgcg
     1741 tccgacaatt gccccgacga ttcgaacgcc gaccagcgcg atttcgatct cgattccctc
     1801 ggcgatccgt gcgatccgga ccgcgacggc gacgggctgc ccaacctcgt cgacaactgc
     1861 cccgacgacc ccagtgccga tcagctcgat ctcgatctcg acgggctcgg cgacatctgc
     1921 gaccccgacg atgacggaga cgggctgctc gacagcgtcg agaccggaac cggcatctac
     1981 gtttcgcccg aagacaccgg caccgacccg cggcgcgcgg ataccgatgg cgacggagtc
     2041 agcgacggtg acgaggtcaa cggtgagatc ttcaccgacc cgaacgaccc cgacgacaac
     2101 cccactgcga tcccagcggt ctccggctgg gggatgctcg tgctcggcgc gctgctgctc
     2161 gcggtcgccg cggcgttccg gctgcgcgcg cgccgcttgc gttcgacccg gtgaactcgc
     2221 ccgcgccagc ggcggattcc tgattggaaa gcggaacggc ccccggtcga acccgtgcgg
     2281 tgaactgggc cccggccagc ggcggatttc tgataagaaa gcggaactgc ccgggctaga
     2341 tgttgggcat cgaaatcgct cggatcgacg tcgactgcgg atcgcgcgcg gccggcgcgc
     2401 gcgcgggcgc atcgctgcgg ccggtgcgcg cccgaagtgt gcgcacgccg atcgcgtagc
     2461 tcgccgcggg cagcgcgaag ctcgcgatcg aagcaggcag gaaccggaag atctcggtga
     2521 cctccgacgt gagcgggttg tagagagaca ggtagacggc ctgcccgagc acgacggatg
     2581 cgagcgccac ccagttgtag ccgcgcgcgt agtggtaggc ggcgctcggg tcgtcgctga
     2641 agatcgatcg cagatcgagc ttctgtcgtc gcagcaggaa gtaatccgtg aacaggattc
     2701 ccgcgaccgg cgcgaacagc gtgccgttgt aggtgaggaa cgcgtcgccg agatcgtaga
     2761 gctgctgcgg ccagacgatg aacggcaggc acggcacgac ggtgagctgc atcagcgtcg
     2821 accagggcat caaccggaag cgctcgatgt gccgcagcgc gagcccgctc gcgaacatcg
     2881 agaccgagct ggaggtcacg ttggcgagcg cgacgaagca cagtgcgagc acgccgagca
     2941 cgggtccgcc gagcgggacc atccattcgg tcgggtcgtc ggactgcacg accagtgccg
     3001 agaacagcgc caccgcgcac gcgagcgcct ggaacaggcc catctgcagc agcaccgggt
     3061 agaccgcgtt gcgacgcgtc cgcgtgttgc gcgcgaggaa gccgagccct ccccaccagc
     3121 cgatgccgcc ggcgatgccg atctcgaccg cgaccacgta gttgacgatc ggattcggaa
     3181 acggacgcag cggctcggcg gcgaggatct cgcgccagcc gtgctcggcg atcagcatgt
     3241 agagcatgaa cgcgatcagc gcggcgaggc ccggggcgac gatcgcgttc gagacgttga
     3301 gccagtggac gccgcgcgtg acgacgacga acgagatgcc gaggccgagc gcgacgcccg
     3361 atccgacgat ccagctcgcg ggcgtgtaac ccatcgcgag
//
LOCUS       DASPGN010000032         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1410996, whole genome shotgun sequence.
ACCESSION   DASPGN010000032 DASPGN010000000
VERSION     DASPGN010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410996"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="VD788_00960"
     CDS             complement(<1..1457)
                     /locus_tag="VD788_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HXV74860.1"
                     /translation="MGIRPTRVSNDAAGGAAAWLLVLVAATIAAAGVAWVLVTMFERK
                     QEARSPFVRVVEVSEISSDPEPWGLNWPHQFDDYKRTAGDRFYGGSSAMPESKLDAQP
                     WLRRLYAGYAFSIDYREARGHAYMLYDQRVTERVTQRSQSGACLHCHASVPVTYRRIG
                     LEAMGRPADAEALAERFDMDAVIRGFREVSTKPYAEVLAMVAATPDGTPDENEPLFPA
                     PSAADHPAVAMPEGHAGPWEAHPVACIDCHDPKSMALRVTRPGFVLGIAALAASGAEV
                     PHLPSIERWREGSRELPYDPNRDASRRELRSYVCGQCHVEYYCADKMVLTFPWGNGLR
                     ADDLERFWEEQSFPDGSRFYDFRHGETGAPVFKVQHPEFELWSQGVHARSGVSCADCH
                     MPYERQGAMKVSSHNVRSPMQNINSACQQCHNVEESILRENVAAIQSRTMALMDRAAE
                     AMTDMLDAIREAQAAGASEEALAEIFALQRKAMWRL"
     gene            complement(1486..1974)
                     /gene="nrfH"
                     /locus_tag="VD788_00965"
     CDS             complement(1486..1974)
                     /gene="nrfH"
                     /locus_tag="VD788_00965"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HXV74861.1"
                     /translation="MARRRHDRRDTRERGSLPLAAACAVGALAGLGGFVVVYGDGTAY
                     LSSDPAACTNCHVMQGHFDSWQQSTHRHVTVCNDCHLPPGVLDKWLTKTDNGVMHSYA
                     FTTGDFPDPIRIKPRNARRTQRSCLKCHGELVHALGPIDRSGEMPACVRCHASVGHAQ
                     RR"
     gene            complement(2014..>3291)
                     /locus_tag="VD788_00970"
     CDS             complement(2014..>3291)
                     /locus_tag="VD788_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HXV74862.1"
                     /translation="FGAQVVSIVLALAGSVIVARTLGPNGKGVLAVFGLVLQTAAAFG
                     TFGTAFALTFLAGKRRHRPAQLFGASLVLAGLLWLPAAALAAVVEDWALATALTGLSP
                     EAYRLAVALVGPAYAVLLFQAILLGEGRVERVALISTESAAVTTAILLWALIVADRGL
                     DGAIAASAAGTVYAMVRLGAVLARAHGIGFADTRAILRFAVPFGLKGYVGSLASHAWL
                     RADVYLLNALAGPAAVGQYSIATNMAERIWMLEGAIVQSAAPEVIRRDHDRAAELTGR
                     VARHLLVLSVLAAAALAAVSPWIVPLFFGEAFRPAIVPLLILLPGVVALSVARVFSGY
                     YSGQLGRPSTVSLVAGVTALIALGLYVLVIPRWGLNGAAIASTAAYAVPLAIYLFIVP
                     RATGIPVARLLVLSGGDVRELVARTRRLFHPSG"
BASE COUNT          528 a         1192 c         1106 g          465 t
ORIGIN      
        1 agccgccaca tggctttgcg ctgaagcgcg aaaatctccg ccagcgcctc ctcgctcgct
       61 cccgcggcct gggcctcgcg gatggcatcg agcatgtccg tcatcgcctc cgccgcccgg
      121 tccatgaggg ccatcgtgcg cgactggatg gcggcgacgt tttcgcgaag gatgctctcc
      181 tccacgttgt ggcactgctg gcaggcgctg ttgatgttct gcatcgggct ccgcacgttg
      241 tggctggaaa ccttcatggc gccctgtcgt tcgtagggca tgtggcagtc ggcgcagctc
      301 acgccgctgc gggcgtggac gccctggctc cacagctcga actccgggtg ctgcaccttg
      361 aacaccggtg caccggtctc gccgtggcga aagtcgtaga accggctgcc gtccgggaac
      421 gactgctcct cccagaaccg ttcgaggtcg tccgcgcgca acccgttgcc ccacggaaag
      481 gtcaacacca tcttgtccgc gcagtagtac tcgacgtggc actggccgca gacgtacgag
      541 cgcagctcgc gccggctggc gtcgcgattc ggatcgtacg gcagctcgcg cgagccctcg
      601 cgccagcgct cgatgctcgg caggtgcggc acctccgcgc cgctcgccgc cagggcggca
      661 atgccgagga cgaaccccgg tcgtgtcacc cgcagcgcca tcgacttcgg gtcgtggcag
      721 tcgatacagg ccaccggatg agcctcccac ggtcccgcgt gaccctcggg catcgcgacc
      781 gccgggtggt ctgcggccga cggtgccggg aacagcggct cgttctcgtc cggcgtcccg
      841 tcgggcgtcg ccgccaccat cgccagcacc tcggcgtagg gcttggtgct cacctcgcgg
      901 aagccgcgga tcaccgcgtc catgtcgaac ctctcggcca gcgcctcggc gtccgccggc
      961 cggcccatcg cctcgagccc gattcggcgg taggtcaccg gcaccgacgc gtggcagtgc
     1021 aggcaggcgc cggattgcga gcgctgggtc acgcgctcgg tgacccgctg gtcgtagagc
     1081 atgtacgcgt gtccgcgggc ctcgcgatag tcgatgctga acgcgtaacc cgcgtacagc
     1141 cgtcggagcc agggttgggc gtcgagctta ctctcgggca tcgcgctcga gccaccgtag
     1201 aaccggtcgc ccgcggtgcg tttgtagtcg tcgaactggt gcggccagtt gagtccccac
     1261 ggctccgggt cgctgctgat ctcgctgacc tccaccacgc gaacgaacgg cgagcgagcc
     1321 tcctgcttgc gctcgaacat cgtcaccagc acccaggcga cgcctgccgc ggcgatcgtc
     1381 gccgcaacca gcaccagcag ccatgccgcg gcgccccccg cggcgtcgtt cgaaacccgt
     1441 gtcggtcgga tgcccatctc gcctcccccg cgacagacct ggggtctatc gtcgttgcgc
     1501 gtgtccgacg ctggcgtgac agcgcacgca tgccggcatc tccccgctgc ggtcgatcgg
     1561 ccccagcgcg tgcacgagct cgccgtggca cttcagacag ctgcgctgcg tgcggcgcgc
     1621 gttgcgcggt ttgatccgga tcgggtcggg gaaatcgccg gtcgtgaacg cgtacgagtg
     1681 catcacaccg ttgtcggtct tggtcagcca cttgtcgagc acacccggtg gaaggtgaca
     1741 gtcgttacag acggtgacgt gacggtgggt cgactgctgc cacgagtcga agtgaccttg
     1801 catcacgtga cagttggtgc aagcggccgg gtcgctgctc aaataggccg tgccgtcgcc
     1861 gtagacgacg acgaacccgc cgaggccggc gagtgcgccg accgcgcacg ccgcggccag
     1921 cggcaaggag ccgcgctctc gcgtgtcgcg tcggtcgtgg cgccggcgag ccatcgagcg
     1981 gcctccgtga cccgccacga taacatgccc ggttcatccg gacggatgaa acaagcgtct
     2041 cgtgcgcgcc accagctccc gcacgtcccc acccgacagc acgagcagcc gcgcgaccgg
     2101 gatgccggtc gcgcgcggga cgatgaacag gtagatcgcg agcggcaccg cataggcggc
     2161 ggtcgaagcg atcgccgcgc cgttcagccc ccaacgcgga atgaccagca cgtagagccc
     2221 caacgcgatc aacgcggtca ccccggcgac gagcgagacg gtcgatggcc gaccgagctg
     2281 gcccgagtag taaccgctga agacccgcgc gacggaaagc gcaacgacgc cggggagcag
     2341 gatcagcagt ggcacgatcg ccggacggaa cgcctcgccg aagaagagcg ggacgatcca
     2401 cggcgagacc gccgccagcg ccgccgcagc cagcacgctg agcacgagca ggtggcgtgc
     2461 cacccgcccg gtcagctcgg ccgcgcggtc gtggtcgcgc cggatcactt ccggcgccgc
     2521 ggattggacg atcgcaccct cgagcatcca gatccgctcg gccatgttgg tcgcgatcga
     2581 gtattgtccg acggccgcag gaccggcgag cgcattcaac aggtagacgt ccgcgcgcag
     2641 ccaggcgtgg ctggccagcg agccgacgta ccctttcagt ccgaacggca cggcgaatcg
     2701 gaggatcgcg cgcgtgtcgg cgaagccgat gccgtgggcg cgcgccagca ccgcgcccag
     2761 gcggaccatc gcgtagaccg tccccgcggc actggccgcg atcgctccgt cgaggccgcg
     2821 atccgcgacg atcagcgccc agagcaggat cgcggtcgtg accgccgcgc tctcggtgct
     2881 gatcaacgcg accctctcca ctcgtccttc ccccagcagg atcgcctgga acagcagtac
     2941 cgcgtaggcc ggtccgacga gggcgaccgc gaggcggtag gcttccggac tcagaccggt
     3001 caacgccgtc gccagcgccc agtcctcgac gaccgccgcc agtgccgcgg ccggcagcca
     3061 caacaagccc gccagcacga gcgaggcccc gaacagctgc gccggacgat ggcgtcgctt
     3121 cccggcgaga aaggtcaggg cgaacgccgt gccgaaggtg ccgaacgcgg ccgcggtctg
     3181 caacaccaga ccgaagacgg cgagcacgcc cttgccgttc ggccccagcg tgcgcgcgac
     3241 gatgaccgag ccggccagcg cgagcacgat cgacacgacc tgcgcaccga a
//
LOCUS       DASPGN010000033        18320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_591578, whole genome shotgun sequence.
ACCESSION   DASPGN010000033 DASPGN010000000
VERSION     DASPGN010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 18320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18320
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591578"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4173
                     /locus_tag="VD788_00975"
     CDS             <1..4173
                     /locus_tag="VD788_00975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV74863.1"
                     /translation="VDLACLSYGLVDVMVPGNATEAQVGFILRHAAARTVVVSGASQL
                     RKIAVNRKALPALRHVVTLQPTADRGERVLTLDRLIQLGTGVDADQLDRRRNAVRVDD
                     LATLMYTSGTTGTPKGIQFSQRNLVFKRFARGLAIPGIGDGDVFLCFLPLFHTFGRFL
                     EMLGCVFWGATYCFLDNPSIESLIGAMGRVRPSVFISVPKKWIQLYESIAALADPVQA
                     SDAELSAATQRLTGGRLRWGLSAAGHLDPDVFRFFQRQGVELMSGFGMTEATGGITMT
                     PPGEYRDDSLGLPLPGIELKLAPDGELCIRGPYVMMGYLDEDGSETSFDDEGWLHTGD
                     LMQQDADGHLRLVDRKKEIYKNIKGETIAPQRIENMFRDFESVGRAFLVGDHRENNTL
                     LIHPNYQYKELDFASLPPQQVKDHFRSLVVSVNKFLAPFERIVDFAVVERDLSADHGE
                     LTAKGTPRRKRVVENFRDTIALLYRRTRLDVGGLELNLPNWVLQMVGLTTQDIRVGAD
                     HIDFPQRRGRLTVRPYGRGMTLIGSCIYRHPVGPLNLGDLLSTPGLWLGNEQLVAMVG
                     FGSLARQRPRRGDDRIEWAGRDRPSRPSARDRQTVARARQRDVHELADLHHAALLLAA
                     PETEPAREALQLIASILADEGHRLSQPAQALLLRAADAAHDDVARRAFQILVPATKDD
                     RFAELLERFLTARPAVMNADTREELSRKYLTPAKIDAFVSCAERACLDSAAGADERAG
                     AMLRFLSAYGASHPASYRRLRNFLVRMREFAVDDRVRAEAARSIGALRDGFRSWLGPT
                     SAMAVDTETGREYRWDDVIVFDDEVPDEDRERILAALRDTALLREASFLFAKGILIRL
                     DDIPPGGVWIRLLGRRHGKSVYRLTVQTRFHGALDLALNLNHSLSADLIDDEVGWLMI
                     AAGADDGQPLVEEFGGYWPEQELWSEEFVSGETLNRALRRLARRSDDQERLRQLWPFL
                     AWTGLSAYVDFWHRTGGRWEIADPDLANVIVPTDDYHTGVRIVSVSSRRPHVDVASMM
                     RGFVDDFIEPTVQQYPVLRDVVGWDVVFSSLFEVVGEERGLELMAGLVERDDDHRDSM
                     SAALVRYVDTVRVRGFLPRRLFFAAKRYRRWASLSTDATPQARARTLQELYDTYGLQQ
                     LTRDYPEVRLRYFRETVFREGPEALLTGLDRLIGQLRKRELVGDQLIDAVADLRARLK
                     LDENDDYFLARVSLPYLRPEDAADFVSSDSGGRHQSEIVVALEDHDGNVIRVRHALTP
                     KEVGKLHRLFQAAKVDVTFRPEHRYLVAINDRQQIIGGIYYEIDESGASAHLEKIVVS
                     DAYRRKGVADGLMNELFNRLRSAGMKTVTTGFFRPGYFYSYGFRIEKRYAGLVKSLTE
                     ETTARAGATPAS"
     gene            complement(4166..5479)
                     /locus_tag="VD788_00980"
     CDS             complement(4166..5479)
                     /locus_tag="VD788_00980"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase, eukaryotic"
                     /protein_id="HXV74864.1"
                     /translation="MPTLKKKLADKIPAMREAVRALLADHGDRKISDVSISQAYGGMR
                     GVKSLVTETSALDPDKGIRFRGYSIPEIQRVLPRPAGVDQVLPEGLFHLLLTGEVPSL
                     DEVREITTAWRSHEELPEHTKAALDALPTDTHPMTQFSLGILSLQHESIFAREYRNGV
                     AKQDLWQPMFDDAMVLLGRLPVLAAYIYRRSYKRGRHIPAGGSELDWAANLAHMLGVE
                     ASQFKELMRLYLTIHADHEGGNVSAHTTHLVGSALSDPYLALSAGMNGLAGPLHGLAN
                     QEVVRWIFEMKSELGNAPSKAAIEQYVWQTLDGGRVVPGYGHAVLRQTDPRYMAQREF
                     ALEHLPGDENFRLVSLLYEIVPGILREQGKAKNPWPNVDAHSGCLLIHYGVVEYDFYT
                     VLFGVSRAMGVLSSLVWDRALGLPLERPKSVTTEWLQAQVSETVS"
     gene            complement(5524..7239)
                     /gene="uvrA"
                     /locus_tag="VD788_00985"
     CDS             complement(5524..7239)
                     /gene="uvrA"
                     /locus_tag="VD788_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXV74865.1"
                     /translation="MRRAELEKLMAVRPCSDCRGARLRPESLAVRVGGLDIARHTRMS
                     VAEALTFYESLELTAHELAIAKSILKEIGERLGFLNNVGLSYLTLDRSATTLSGGESQ
                     RIRLATQIGSRLTGVLYILDEPSIGLHQRDNRRLLDTLTQMRDLGNTVIVVEHDEETI
                     RSADYVVDLGPGAGELGGEVVAAGTPSQVARCRRSLTAAYLSGRETIPIPERRREGNG
                     NAITVHGARQHNLKSIDASFPLARLTCVTGVSGSGKSTLVNEILHRALARALHGSEDE
                     PGEHDKITGIGQIDKVIDIDQSPIGRTPRSNPATYVGLFSAVRELFALVPEARMRGYK
                     PGRFSFNVRGGRCESCDGDGVLKIEMHFLPDVYVTCDVCRSHRYNRETLEILYKGRNI
                     ADVLAMTVREALDFFDPVPRIREKLQTLFDVGLGYIRLGQAATTLSGGEAQRIKLSKE
                     LSRRATGRTLYLLDEPTTGLHFDDIKKLLAVLNRLVDSGNTVVVIEHNLDVIKTADWV
                     IDLGPEGGDDGGRIVTQGTPEHVARSTGSHTGAFLSGVLRARLAAGRGQARLTPPSAQ
                     TTLTH"
     gene            complement(7325..7411)
                     /locus_tag="VD788_00990"
                     /pseudo
     tRNA            complement(join(7325..7359,7378..7411))
                     /locus_tag="VD788_00990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /pseudo
                     /anticodon=(pos:complement(7379..7381),aa:Leu,seq:caa)
     gene            7678..7977
                     /locus_tag="VD788_00995"
     CDS             7678..7977
                     /locus_tag="VD788_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74866.1"
                     /translation="MPFGLDHDQPVRHRQRGLDRIGQPALDVGPHDQPVDDDLDRVLF
                     LLVELDLVRQVAHVAVDPRANETLPRRVLELLAVDPLAAADDRRQHLDPAILRCA"
     gene            8478..9173
                     /locus_tag="VD788_01000"
     CDS             8478..9173
                     /locus_tag="VD788_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74867.1"
                     /translation="MKRSSLGTLFQLSLFTVWLSSGVAARPTEPVAALFVPDLDRAGE
                     LTLEGATWARKATDHTVLLRPLDDRERLRYIENRTGVAVDPFSTPPGRQPRYLTYLLV
                     IENRGDSEVGFNALDCWLKTNRDEIRTPVGLTDLSFDYQLAGLTLPAAYERIATALLE
                     GGRIVRPGETISGLLVYHVTHPRTKRFRIEVDLIPSSGEVIRFSAPYRKDGDSGKGST
                     AARGETGAGGEER"
     gene            9173..10108
                     /gene="folD"
                     /locus_tag="VD788_01005"
     CDS             9173..10108
                     /gene="folD"
                     /locus_tag="VD788_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HXV74868.1"
                     /translation="MTAETRGPRTLDGKRTARQIRDEVARGCAALKRDRGIVPGLTVV
                     LVGDDPASEVYVRNKELAAAKAGMSSRIERLPAATSEARLLDLVDRLNVDPQVHGVLV
                     QLPLPGEIDTQRVIQAIDPAKDVDGLHPENAGRLLAGLPGFVPCTPAGVIELLKRHDI
                     PLAGRHAVVIGRSNIVGKPMALLLLRENCTVTVCHSRTRELAEVAARGEILVAAVGRV
                     GLVTAEFIRPGAAVVDVGIHRVTDEDEVRRLFGDDAARLAAVREKGLTLAGDVNPLDA
                     AARAAWLTPVPGGVGPLTIAMLLRNTLESARSATV"
     gene            10135..10749
                     /gene="coaE"
                     /locus_tag="VD788_01010"
     CDS             10135..10749
                     /gene="coaE"
                     /locus_tag="VD788_01010"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXV74869.1"
                     /translation="MLHVGLTGGIAAGKSTVAAELARLGAEVVDADEVVHALLAPQGS
                     AVAPVVARFGQRFRDTSGGIDRAALAELVFADPAARRDLETILHPQVRDEAARRFGRA
                     AKERRCRIGVFDAALLVETGAYRDYDRLIVVRCPPQVQILRLVRRAGLTEQEARARIA
                     AQAPLADKLVVADYVIDTGGTLQQTIEHTHRVWRLLERDARERG"
     gene            complement(10759..11148)
                     /gene="panD"
                     /locus_tag="VD788_01015"
     CDS             complement(10759..11148)
                     /gene="panD"
                     /locus_tag="VD788_01015"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HXV74870.1"
                     /translation="MNRTMLRAKIHRVAVTECDVTYEGSLTLDAELIERAGMLPFERI
                     EVYDVDNANRFSTYLIAGRRGSGECCVNGAAAKLVDVGDKLILASFTTLPDELAVEHR
                     PTIVLVGEGNRVKSHKQTETAGVRVEH"
     gene            complement(11213..12073)
                     /gene="panC"
                     /locus_tag="VD788_01020"
     CDS             complement(11213..12073)
                     /gene="panC"
                     /locus_tag="VD788_01020"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HXV74871.1"
                     /translation="MDIVRRVQSMRETARQERAKGRRIGLVPTMGYLHEGHVGLIRRI
                     HEQADVVVVSIFVNPTQFGPEEDFDSYPRDLTRDTDLCIRENVDYLFTPSSDEIYPPG
                     PRTYVDIPELGTRLEGASRPGHFRGVATVVVKLLNIVRPHVTAFGEKDAQQLAVVRRL
                     IDDLRLGIELLSVPTARDERGLALSSRNRYLSAEQYEAALAIPRALEAARETTSAAEA
                     DAARVTSAARAVLEAEALLETDYVELVHPTTLEPVAGELASGLLLVAVRCGGTRLIDN
                     TMIEPTEQPG"
     gene            complement(12083..12907)
                     /gene="panB"
                     /locus_tag="VD788_01025"
     CDS             complement(12083..12907)
                     /gene="panB"
                     /locus_tag="VD788_01025"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXV74872.1"
                     /translation="MSTFQSQPRRVMTVPRFVKRKRIGPPLVMLTAYDLPTARAAEAG
                     GVDALLVGDSLGNVLLGERDTLTVTLDQMIHHARAVGRARQSALLIVDMPWMTYHVSI
                     EETLRNAARIIRETSADAVKLEGGANRVPAIRALLDAEIPVMGHLGLTPQSVLRMGGY
                     KVQGRSEAAVERLVEDAQRIEQAGVFGLVLEGVPSAVAARITGTVAVPTIGIGAGDHC
                     DGQVLVFHDLLGMSPEPGPKFVRRYAEIHDAQVDAIRRWAGDVRQRTFPGDAESYG"
     gene            complement(12969..13607)
                     /locus_tag="VD788_01030"
     CDS             complement(12969..13607)
                     /locus_tag="VD788_01030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HXV74873.1"
                     /translation="MKFSTIAVEGPIGVGKTSFVNLLAKKFDAQVVLENLDNPFLKNF
                     YEDEPGAAFQVQLFFLLLRYRQLQELSQRDLFHQVTICDYIFPKDKIFAYLNLDDSEL
                     LIYDKLYAMLEDQVPKPDLVIFLQAETRTLVERIARRNRDYEAQISETYINEVNKAYN
                     YFFFHYAQTPLLVIDTTSIDFVHHGEHLDELVEQIRKMDRGVQYYRPLGSSA"
     gene            complement(13639..14148)
                     /gene="folK"
                     /locus_tag="VD788_01035"
     CDS             complement(13639..14148)
                     /gene="folK"
                     /locus_tag="VD788_01035"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HXV74874.1"
                     /translation="MIGYIALGSNLGDRGANLAAALRRLSASGLPPYASSTVWETEPV
                     DSPSPRWFWNMAVEVRTDRPPLELLDTLLSIERQAGRERTERNAPRPIDLDLLWLGGI
                     ELEHDRLSLPHPRMWRRRFVLGPLAEIAPDLVNEASGRSVAEELDALPRNPRAQRLGA
                     LASCGRLPL"
     gene            14309..14848
                     /locus_tag="VD788_01040"
     CDS             14309..14848
                     /locus_tag="VD788_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV74875.1"
                     /translation="MTTTDKKPADNASHHQQYEEALNRFGVAIGLLQSGAVDEARQAF
                     IELERANAEEPALADRARSYRKICERRLAPPPAPPQDADGLYVAGVIASNNGDYDEAL
                     ALLERALRQTPDSPKVLYARASTWSLKGNAEAAVAELRRAIAVDPRVRFQAANDPDFE
                     PIREEPAFIDIIEPTPSGA"
     gene            14852..16267
                     /gene="glmU"
                     /locus_tag="VD788_01045"
     CDS             14852..16267
                     /gene="glmU"
                     /locus_tag="VD788_01045"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HXV74876.1"
                     /translation="MAATGGGLRVLVLAAGRGKRLRSKSIKLVHSVAGSPMVAHVLDA
                     VARLRPQRIVTVIGFQADEVRAALDGHRSAFVVQREQRGTGHAVLQAARAVGASGDGP
                     LLIVNGDMPTIRPSTLRRFVSHHRRSRAALSFMTAELDEPTGYGRVVRDGGGRVVRVV
                     EQADATAAEKRIREVNCGIYCASPATLFRTLRRLTPDNAQGEYYLTDAVHVLLEDGGA
                     VAAVRHDDAEELLGVNTRQELSRASRTLYARKAEALQDQGVTLLDAERSWIDPRARIG
                     RDTVIYPGVLIEGETVIGRDCVIGLGCRIADSTIGDDVAVRDYSLLERARVGTRAVVG
                     PFARLRPDSWLDPDSRVGNFVELKKTRLGRGSKANHLAYLGDATIGPDCNVGAGTITC
                     NYDGRNKFPTVLGRGVFIGSDTQLVAPVRLGDEAYVGAGSTVTEDVPAGALAISRQPQ
                     TNVEGWVGRRKRRDRARSTKK"
     gene            16373..18214
                     /gene="glmS"
                     /locus_tag="VD788_01050"
     CDS             16373..18214
                     /gene="glmS"
                     /locus_tag="VD788_01050"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXV74877.1"
                     /translation="MCGIVGYIGAKNPVEVLVEGLRRLEYRGYDSAGVAVVDRAGVLG
                     IRRAPGKLRDLERVLAADPLDGVYGIGHTRWATHGRPSEENAHPHRDCSGRLVVIHNG
                     IIENYVELKHDLERRGHRFVTETDTEIVAHALEEELKNGADDLVVALRRVLPTLRGIY
                     ALVAMSADQPQTVVGARQGPPLVVGIGKGEFFLASDIPAILTHTKDVVFMDDGEVVVL
                     RPSGATFLDAEGRSVERPVTRVPWDPIMAEKGGYKHFMLKEIHEQPRAVRDTLLGRVS
                     LESAAIHLDEIGLSDDYLRKVQRMAIVACGTSWHAGLVGKFWIEHLAGLPVEVDYASE
                     FRYRSPLVDKLTLPLYISQSGETADTLAALREAKRRGAETLAICNVQGSMVTREASGT
                     IYTHAGPEIGVASTKAFTSQLVAVILLALKLGSARGRLDERSMLEIVRALYHIPAQME
                     QHLGDDSAIVELAKTFHNRRDFLYLGRGVNYPIALEGALKLKEISYIHAEGYPAGEMK
                     HGPIALIDEEMPVVAVAPHDSVYEKMLSNIEEVKARSGIVLAVTDRDDADLAHKVDAV
                     IRVPRTHELLSPLLTALPMQLLAYHIALLRGCDVDQPRNLAKSVTVE"
BASE COUNT         2964 a         6092 c         6210 g         3054 t
ORIGIN      
        1 gtcgatctgg cctgcttgag ctacgggctg gtcgacgtga tggtgccggg caacgcgacc
       61 gaggcccagg tcgggttcat cctgcgacac gccgcggcgc gaaccgtcgt cgtgtcggga
      121 gcgtcccagc tgcgcaagat cgcggtcaac cgcaaggcgc tcccggcact gcgccacgtg
      181 gtgacgctgc agccgaccgc ggaccgcggc gagcgcgtgt tgacgctcga ccgactgatc
      241 cagctcggaa ccggcgtcga cgcggaccag ctcgaccgcc ggcgcaacgc ggtgcgcgtc
      301 gacgatctcg cgacgctgat gtacacgtcg ggaacgaccg gtacgccgaa gggtatccag
      361 ttctcgcaac gcaacctggt gttcaagcgg ttcgctcgcg gactggccat tcccggtatc
      421 ggcgacggtg acgtcttcct ctgttttctt ccgctgtttc acacgttcgg ccggttcctc
      481 gagatgttgg gatgcgtctt ctggggcgcg acctactgct ttctcgacaa cccgtcgatc
      541 gagtccctga tcggcgcgat ggggcgtgtc cggccgagcg tcttcatcag cgtgccgaag
      601 aagtggatcc agctttacga gtcgatcgcc gcgctggccg atcccgtgca ggcgagcgac
      661 gccgagctga gcgcggcgac gcagcgcctg accggcggcc ggctgcgctg gggtttgtcg
      721 gccgcgggtc atctggatcc cgacgtcttt cggttcttcc agcgtcaggg tgtcgagctg
      781 atgagcgggt tcggcatgac cgaagccacg ggtggtatca cgatgacgcc gcccggggag
      841 taccgtgacg actcgctcgg cctgccgctg ccggggatcg aactcaagct cgcgcccgac
      901 ggcgagttgt gcatccgcgg accgtacgtg atgatgggtt acctcgacga ggacggctcc
      961 gaaacgtcgt ttgacgacga aggatggttg cacaccggcg atctgatgca acaggacgcc
     1021 gacggtcacc tgcgtctggt cgaccgcaag aaggaaatct acaagaacat caagggcgag
     1081 acgatcgctc cccagcggat cgagaacatg tttcgcgatt tcgaatcggt cggacgggcg
     1141 ttcctcgtcg gcgatcaccg ggaaaacaac acgctgctga tccacccgaa ctaccagtac
     1201 aaagagctcg acttcgcctc gctgccgccg cagcaggtca aggatcactt ccgttcgctg
     1261 gtcgtttcgg tcaacaagtt cctcgccccg ttcgagcgca tcgtcgactt cgcggtggtc
     1321 gagcgcgacc tgtccgccga ccacggagaa ctgaccgcca aaggcacgcc gcggcgcaaa
     1381 cgggtggtcg agaacttccg cgacacgatc gcattgctct accgccgcac gcggctcgac
     1441 gtcggcgggt tggagctgaa cctgcccaat tgggtgctgc agatggtcgg gctgacgacg
     1501 caggacatcc gagtcggtgc ggaccacatc gactttccgc agcgccgggg acggttgacc
     1561 gtgcggccgt acggtcgcgg catgacgctg atcggctcgt gcatctaccg ccatccggtc
     1621 ggaccgctca acctgggcga cctgctcagc acgcccgggc tctggctggg caacgagcaa
     1681 ctggtcgcga tggtcggttt cggctcgctc gcccgacaac ggccgcggcg cggggacgac
     1741 cggatcgaat gggccggccg cgacagacca tccaggccgt cggcgcgcga ccggcagacg
     1801 gtcgcgcgtg cccgccagcg cgacgttcac gagctcgccg acctgcatca cgcggccctg
     1861 ttgctcgcag caccggagac cgagccggcg cgggaggccc tgcaactgat cgcctcgatc
     1921 ctcgccgacg agggtcatcg actgtcccag cccgcgcagg ccttgctgct gcgcgccgcg
     1981 gacgcggcgc acgacgacgt ggcgcggcgg gcgttccaga tcctcgtacc ggcgaccaag
     2041 gacgatcgct ttgccgagtt gctcgaacgg ttcttgacgg cgcggccggc agtgatgaac
     2101 gccgacaccc gcgaggaact gtcgaggaag tacctgaccc cggcgaagat cgacgccttc
     2161 gtctcctgcg ccgagcgcgc ctgcctcgac tccgcggccg gggcggacga acgggcgggg
     2221 gcgatgctgc ggttcctgtc ggcctacggc gcgtcgcatc cggccagcta tcgccgtctg
     2281 cgcaattttc tcgtgcggat gcgggagttc gccgtcgacg accgcgtccg ggccgaggcg
     2341 gcccggtcga tcggagcgtt gcgcgacggg ttccgctcgt ggctcggtcc tacctcggcg
     2401 atggcggtcg acacggagac cggtcgcgag taccgctggg acgacgtgat cgtcttcgac
     2461 gacgaggtgc cagacgagga ccgcgagcgc atactcgccg cgctacgcga cacggccctg
     2521 ttgcgtgaag cgtcgttcct gttcgcgaag ggtatcctga tccggctcga cgacatcccg
     2581 ccgggcgggg tgtggatccg gctgctcggc cgccggcacg gcaagtcggt ctaccggctg
     2641 acggtgcaga cgcggttcca cggcgcactc gatctggcgc tcaacctcaa ccattcgctg
     2701 tcggcggatc tgatcgacga cgaggtcggc tggctgatga ttgccgccgg cgcggacgac
     2761 ggacaaccgc tggtcgagga gttcggtggc tactggccgg agcaggagct gtggagcgag
     2821 gagttcgtca gcggcgagac gctgaatcgc gcgctgcggc ggttggcgcg gcgcagcgac
     2881 gaccaggaac gactgcgcca gctttggccg tttctcgcct ggaccgggct gtcggcatat
     2941 gtcgatttct ggcaccgcac cggcggacgg tgggagatcg cggaccccga cctggccaac
     3001 gtcatcgttc cgaccgacga ctatcacacc ggtgtgcgaa tcgtctcggt ctcgtccagg
     3061 cgtccgcacg tcgacgtcgc gtcgatgatg cgcggcttcg tcgacgattt catcgagccg
     3121 accgtgcagc agtacccggt gctgcgcgac gtcgtcggtt gggacgtcgt tttctcgtcg
     3181 ctgttcgagg tggtcggcga agagcgtggt ctcgagctga tggcggggct ggtcgaacgg
     3241 gacgacgacc accgcgactc gatgtccgcc gcgctcgtcc gctacgtcga cacggtacgc
     3301 gtgcgcggct tccttccacg ccgactgttc ttcgccgcca agcgctaccg gcgctgggcg
     3361 tcgctgagca ccgacgcgac gcctcaggcg cgagcgcgga cgctgcagga gctgtacgac
     3421 acgtacgggc tacagcagct gacccgtgat tatcccgagg ttcggctgcg ctattttcgc
     3481 gagaccgtct ttcgcgaggg gcccgaagcg ttgctcaccg gcctcgatcg gctgatcggc
     3541 cagctgcgca aacgagagtt ggtcggcgat caattgatcg acgccgtcgc cgatctgcgg
     3601 gcgcgactca agctcgacga gaacgacgat tacttcttgg cgcgagtgtc gctaccctac
     3661 ctccgtccgg aggacgccgc cgacttcgtc agcagcgaca gtggcggccg gcaccagagc
     3721 gagatcgtcg tcgcgctgga agaccacgac ggcaacgtga tccgcgtccg ccacgcactg
     3781 acgcccaagg aggtgggcaa gctgcaccgg ctgttccagg cggccaaggt cgacgtcacc
     3841 tttcgtcccg agcatcgcta cctggtggcg atcaacgacc gacagcagat catcggcggg
     3901 atctactacg agatcgacga gtccggagcg tcggcccacc tggagaagat cgtcgtcagc
     3961 gacgcctacc gccgcaaggg agttgccgac ggactgatga acgagctgtt caaccggctg
     4021 cgcagcgccg gcatgaagac ggtcaccacc ggcttcttcc ggccgggcta cttctactcg
     4081 tacgggttcc gcatcgagaa gcgctacgcc ggcctggtca agtcgctgac cgaggagacc
     4141 acggctcgag ccggcgcaac gccggctagc tgacggtctc ggagacctgt gcctgcagcc
     4201 actcggtggt gacgctcttc ggccgctcga gcggcagtcc gagcgcccgg tcccacacca
     4261 gcgacgagag cactcccatc gcgcgcgaca cgccgaacaa cacggtgtag aagtcgtact
     4321 cgacgacccc gtagtggatc agcaggcagc cggagtgggc gtcgacgttg ggccacgggt
     4381 tcttggcctt gccctgttcg cgcaggatcc cggggacgat ctcgtacagc aggctgacca
     4441 gccggaagtt ctcgtcgccc ggtagatgct cgagcgcgaa ctcgcgctgg gccatgtagc
     4501 gcgggtcggt ttgacgcagc accgcgtggc cgtaacccgg cacgacccga ccgccgtcga
     4561 gcgtctgcca aacgtattgc tcgatcgcgg ccttgctcgg ggcgttgccg agctccgact
     4621 tcatctcgaa gatccaacgg accacttcct ggttcgccag cccgtgcagc ggcccggcca
     4681 acccgttcat tccggcggac agcgccagat acggatcgga caacgcggat ccgacgaggt
     4741 gtgtcgtgtg cgcgctcacg ttgccgccct cgtggtcggc gtggatcgtc agatacagcc
     4801 gcatcagctc cttgaactga ctggcctcga cccccaacat gtgcgcgagg ttggccgccc
     4861 agtcgagctc ggatccgccg gcggggatgt gccgtccgcg cttgtagctg cgccggtaaa
     4921 tgtaggcggc gagcacgggc aggcgtccga gcagcaccat cgcgtcgtcg aacatcggtt
     4981 gccaaaggtc ttgcttggcg acaccgttgc ggtattcccg ggcgaagatc gactcgtgct
     5041 gcaacgacag aatgccgagc gagaactggg tcatcgggtg cgtgtcggtc ggcagcgcgt
     5101 cgagcgcggc cttggtatgc tcgggcagct cttcgtggct gcgccaggcg gtggtgatct
     5161 cccgcacctc gtcgagcgac gggacctcac cggtcagcag cagatggaac aacccctccg
     5221 gcagcacctg atcgacgcct gcgggtcggg gtaggacccg ttggatctcc ggaatgctgt
     5281 aaccgcggaa gcggatcccc ttgtccgggt cgagcgccga ggtctcggtg accaggctct
     5341 tcaccccgcg catcccgccg taggcctggg agatcgacac gtcggagatc ttgcggtcac
     5401 cgtggtcggc gagcagcgcg cgcacggcct cgcgcatcgc ggggatcttg tcggccagct
     5461 ttttcttgag cgttggcatc ggcgtcctcc tgcgacgtca cagcgcgaag cggtcgaggg
     5521 gtctcaatga gttagagtag tttgggccga cgggggtgtc aaccgagcct gtccgcgccc
     5581 agcagccaaa cgcgctcgta agactccaga aaggaacgct ccggtgtgag agccggtgct
     5641 gcgggcgacg tgctcgggtg tgccctgggt gacgatgcgt cctccgtcgt ccccaccttc
     5701 ggggcccagg tcgatcaccc agtcggcggt cttgatcacg tcgagattgt gctcgatgac
     5761 gacgacggtg ttcccggaat cgacgaggcg gttgaggacg gcgagcagtt tcttgatgtc
     5821 gtcgaagtgc aatcccgttg ttggctcgtc gagcaggtac aacgtgcgcc cggtcgcgcg
     5881 gcgcgacagc tccttggaca gcttgatgcg ctgggcttcg ccgcccgata acgtggtggc
     5941 ggcctgtccc aggcggatgt accccaggcc gacgtcgaac agcgtctgga gcttctcccg
     6001 gatccgcggg acggggtcga agaagtcgag cgcctcgcga acggtcatcg ccagcacgtc
     6061 ggcgatattc cgccctttgt agaggatctc cagcgtctcg cggttgtagc ggtggctccg
     6121 gcaaacgtcg caggtgacgt agacgtcggg caggaagtgc atctcgatct tgagcacgcc
     6181 gtcgccgtcg caggactcgc aacggccgcc ccggacgttg aacgagaatc gaccgggctt
     6241 gtagccgcgc attcgggcct ccggcaccag tgcgaacagc tcccgcacgg ccgagaacag
     6301 cccgacatag gtggccgggt tggagcgtgg tgtgcggccg atcggtgatt ggtcgatatc
     6361 gatcaccttg tcgatctgtc cgattcccgt gattttgtcg tgctcgcccg gctcgtcctc
     6421 cgacccgtga agcgcacggg ccaacgcgcg gtggagaatc tcgttgacca acgtgctctt
     6481 gcccgacccg gaaacgccgg tgacgcaggt gagtcgggcc agcggaaaac tggcgtcgat
     6541 cgacttgagg ttgtgctgcc tcgccccgtg aaccgtgatc gcgttgccgt tcccctcccg
     6601 tcgccgttcg gggatcggga ttgtctcgcg gccggacagg taggctgcgg tcagcgaccg
     6661 tcgacagcgc gccacctgtg acggcgttcc ggcggcgacg acctcaccgc ccagctcgcc
     6721 ggcccccggc cccagatcga cgacgtagtc ggcgctgcga atcgtctcct cgtcgtgctc
     6781 gaccacgatc accgtgttgc ccaggtcgcg catctgcgtc agcgtgtcga gcagccggcg
     6841 gttgtcgcgc tggtgcaatc cgatcgacgg ctcgtcgagg atgtagagca cgccggtcaa
     6901 ccgcgacccg atctgggtcg ccaagcgtat gcgctggctc tcgccccccg acaacgtcgt
     6961 cgccgatcga tccaaggtca ggtagctcag cccgacgttg ttgaggaaac ccagtcgctc
     7021 gccgatctcc ttgaggatcg acttggcgat ggcgagctcg tgggcggtaa gctcgagcga
     7081 ctcgtagaac gtcagcgcct cggcgacgga catccgcgtg tgccgggcga tgtcgagacc
     7141 tccgacccgc accgcgagac tttcggggcg caaccgggcg ccccggcaat cggagcacgg
     7201 ccggaccgcc atcagctttt cgagctcggc gcggcgcatc tcggaggtcg tctcgcgata
     7261 gcgccgctcg aggatcgcca gcgcccctct ccaacgcttg cgaaactcgt aggtcgagcg
     7321 tgtgccctgc cagcgaaagt cgaactcgcg accgccggca ccctcccaaa acaccttttt
     7381 gaagcgggcc ggccacttgc ggtacggcag cccgggatcg accttgtacg agcggaacag
     7441 gctcgcctcc agcatctggt accagccggc gtcgccccag gccagcgccc cgtcacggag
     7501 cgcacggccg tcgtcgacga tcagacgcga cttggcgaac gacttcttga cgcccagccc
     7561 gtcgcaaccc gggcaagcgc cgtacggcga gttgaacgag aacatgcggg gcgccaactc
     7621 ggggacggag acgccgcagt tgggacaggc gagatgacgc gagaacagta ggtcgccttg
     7681 cccttcggcc tcgatcacga ccagcccgtc cgccaccgcc agcgcggtct cgaccgaatc
     7741 ggtcagccgg ccctggacgt cgggccgcac gaccaaccgg tcgacgacga tctcgatcgt
     7801 gtgctttttc tgcttgtcga gctcgatctc gtccgccagg tcgcgcacgt cgccgtcgac
     7861 ccgcgcgcga acgaaaccct gccgcgccgc gtcctcgagc tccttgcggt agatcccctt
     7921 gcggccgcgg acgatcggcg ccagcacctg gatcctgcga tcctccggtg tgcgtagcac
     7981 cgcatcgacg atctgctgga cggactggga gcgaatcgcc ttgccgcact tgtggcagtg
     8041 cggccgcccg gcccgcgcga acagcagccg caggtaatcg tagatctcgg tcaccgtgcc
     8101 gacggtcgag cgcgggttgc gcgccgtcgt cttctgctcg atcgagatcg ccggcgacag
     8161 gccctcgatc gattcgactt cgggcttttc catctgttcg aggaactgac gcgcgtaggc
     8221 cgacaacgat tcgacgtagc gccgctggcc ctcggcgtac aacgtgtcga acgcgagcga
     8281 ggacttgccc gacccggaga cacccgtgat caccaccagc ttgttgcgcg gtagcgtcag
     8341 gtcgatacgt ttgaggttgt gttcgctcgc tccgcgaatt acgatctcgc cggtactcat
     8401 gcggcgtgga ttctatcgcc attcggcggt tttgtccgac cggatcgggc gcaacccgct
     8461 gtgctattct cccgcgggtg aagcggagct ccttgggcac gttgtttcag ctgtcgctgt
     8521 tcaccgtctg gttgtcgagc ggggttgcgg cccggccgac cgagcccgtc gccgctttgt
     8581 tcgtgccgga cctcgaccgt gccggtgagc tgacgctcga gggcgcgacc tgggcccgca
     8641 aggccaccga tcacacggtg ctgctgaggc cgctcgacga tcgggagcgc ctgcgctaca
     8701 tcgagaaccg gaccggcgtc gccgtcgatc cgttctcgac gcctccgggt cgccagccgc
     8761 gctacctgac ctacctgctc gtgatcgaga atcgaggcga cagcgaagtc ggattcaacg
     8821 cgctcgactg ctggttgaag accaatcgcg acgagatccg cacgccggtc gggctgaccg
     8881 acctcagctt cgactaccag ctcgccggat tgacgctgcc ggcggcctac gaacggatcg
     8941 cgacggcgtt gctcgagggc ggacggatcg ttcggcccgg cgaaacgatc agcgggctgt
     9001 tggtctacca tgtgacccac ccgcggacaa agcgctttcg gatcgaggtc gacctgatac
     9061 cgtcgtccgg cgaggtgatc cgcttcagcg cgccgtaccg gaaggacggt gattccggca
     9121 agggctcgac cgcggcgcgg ggcgagaccg gtgcgggagg cgaggagcgg tgatgacggc
     9181 cgagacgcga gggccgagga cgctcgacgg caagcggacg gcacggcaga tccgggacga
     9241 ggtggcccgc ggctgcgccg cgctgaagcg cgaccgagga atcgtcccgg gactgaccgt
     9301 cgtgctggtc ggcgacgacc cggcgtccga ggtctacgtc cgcaacaaag agctcgcagc
     9361 ggccaaggcc gggatgagct cgcggatcga acgtctgccg gccgcgacca gcgaggcgcg
     9421 tctgctggac ctcgtcgacc ggttgaatgt cgatccgcag gtccacggag tcctggtcca
     9481 gctcccgctt cccggcgaga tcgacacgca gcgcgtgatc caggcgatcg atccggcgaa
     9541 ggacgtcgac gggctgcacc cggagaacgc cgggcgtttg ctggccgggc ttcccggttt
     9601 cgttccgtgc acaccggccg gcgtgatcga gctgctgaag cgccacgaca tcccgctcgc
     9661 cggccgccac gcggtggtga tcgggcgcag caacatcgtc ggcaagccga tggcgctctt
     9721 gctgctccgc gagaactgca ccgtcaccgt ctgccactcg cgcacccgcg agctggccga
     9781 agtcgccgcc cgcggcgaga tcctggtggc cgcggtcggt cgcgtgggat tggtcacggc
     9841 ggagttcatc agaccgggtg cggcggtcgt cgacgtcggc atccatcggg tcaccgacga
     9901 ggacgaggtg cgacggctgt tcggcgacga cgcggcgcgc ctcgcggcgg tgagagagaa
     9961 gggcctgacg ctggcgggcg acgtcaaccc gctggacgcc gccgctcgtg cggcgtggtt
    10021 gaccccggtg cccggcggcg tcggcccgct gacgatcgcg atgctgttga ggaacacgct
    10081 cgaatccgca cgtagcgcga cggtctgaaa cgcgccgtgt gcccagcgcc ggcaatgctc
    10141 cacgtcgggc tcaccggcgg tatcgccgcc ggcaagtcga cggtcgcggc cgagctggcc
    10201 cgactcggcg ccgaggtggt cgacgccgac gaggtggtgc acgcgctgct ggcgccccag
    10261 gggtcggccg tcgccccggt cgttgctcgc ttcggccagc gattccgcga cacctcgggc
    10321 ggcatcgatc gcgccgcgct cgccgaactg gtgttcgccg acccggccgc gcgacgcgac
    10381 ctcgagacga ttctccaccc gcaggtgcgc gacgaggcgg cgcgccgctt cggccgggcc
    10441 gcgaaagaac gacgttgccg gatcggcgtc ttcgacgccg cgttgctcgt cgagaccggg
    10501 gcgtaccgcg actatgaccg gttgatcgtc gtgcgctgtc cgccgcaggt ccagatcttg
    10561 cggctcgtgc gtcgcgccgg actgaccgag caggaggcac gtgcgcgcat cgccgcccag
    10621 gcgccgctgg cggacaagct ggtcgtcgcc gactacgtca tcgataccgg cggcacgctg
    10681 cagcagacca tcgagcacac gcatcgggta tggcgtctgc tcgagcgcga cgcgcgcgag
    10741 cgtggctgac ctcgcccgtc agtgctcgac acgcacgccg gccgtctcgg tctgcttgtg
    10801 cgatttgacg cgattcccct cgccgaccaa cacgatcgtc ggccggtgct cgaccgccag
    10861 ctcgtcgggt agcgtggtga acgaggcgag gatcaacttg tcgccgacgt cgaccagttt
    10921 cgccgccgcc ccgttgacgc agcattcgcc gctgccgcgc cggccggcga tcaggtaggt
    10981 cgaaaaacgg ttggcgttgt cgacgtcgta gacctcgatg cgctcgaacg ggagcatccc
    11041 ggcacgctcg atcagctcgg catcgagcgt cagactgcct tcgtaggtca catcacattc
    11101 ggtcacggcg acgcggtgga tcttggcgcg cagcatcgtt cggttcatct tcggctcctc
    11161 tgcgagtcgc cctccactgc gggatggacg ctcgacacct agggctcggg gctcacccgg
    11221 gctgttcggt cggttcgatc atcgtattgt cgatcagacg tgttccgcca caacggacgg
    11281 cgaccagtag caacccgctc gcgagttcgc cagcgaccgg ctcgagcgtc gtgggatgga
    11341 ccaactcgac gtagtccgtc tcgagcaacg cctcggcctc gagcacggcc cgcgcggcgg
    11401 aggtaacccg agcggcgtcc gcctcggcgg ccgacgtcgt ttctcgcgcc gcctccagtg
    11461 cgcggggaat ggccagcgcc gcctcgtatt gctcggccga caggtagcgg ttccgactcg
    11521 acaacgccag tccgcgttcg tcgcgggcgg tcggcacgct cagcagttcg atacccagcc
    11581 gcagatcgtc gatcagccgg cggacgacgg cgagctgctg ggcgtccttc tcgccgaacg
    11641 cggtgacgtg cggccgcacg atgttgagca gcttgaccac gaccgtcgcc acgccgcgga
    11701 aatggccggg tcggctcgcc ccctcgagcc gtgtccccag ctcggggatg tcgacgtagg
    11761 ttcgcggacc cggcgggtag atctcgtcgg acgacggcgt gaacaggtaa tcgacgtttt
    11821 cgcggatgca caggtcggta tcgcgtgtca ggtcgcgtgg gtagctgtcg aagtcctcct
    11881 ccgggccgaa ctgcgtggga ttgacgaaga tcgacaccac gaccacgtcg gcctgttcgt
    11941 ggatccgccg aatcagcccg acgtggcctt cgtgcaggta acccatcgtc ggcacgagac
    12001 cgatccgccg gcccttggcg cgctcctggc gtgcggtctc ccgcatcgac tgaaccctgc
    12061 ggacgatatc catcgcgtcg tgtcagccgt agctctcggc gtcgccggga aaggtgcgtt
    12121 ggcgtacgtc gcccgcccaa cgccggatcg cgtcgacctg ggcgtcgtgg atctcggcgt
    12181 agcgccggac gaacttcggc ccgggctcgg gcgacatgcc gagcaggtcg tggaacacca
    12241 gcacttggcc gtcgcagtgg tcgccggcac cgatgccgat cgtcgggacc gcgacggttc
    12301 cggtgatgcg tgccgcgacc gcggacggta cgccttcgag caccagtccg aacacgcccg
    12361 cctgttcgat ccgttgcgcg tcctcgacca gccgctcgac cgccgcttcg ctgcgccctt
    12421 gcaccttgta gccacccatg cgcagcaccg actgcggcgt cagcccgagg tggcccatca
    12481 ccggaatctc cgcatcgagc aacgcgcgga tcgccggcac ccgattggcg ccaccctcga
    12541 gcttgaccgc atcggcgctg gtctcgcgga tgatgcgggc ggcattccgc agcgtctcct
    12601 cgatcgacac gtggtaggtc atccacggca tgtcgacgat gagcaacgcg ctctgccggg
    12661 ctcgcccgac ggcacgcgcg tggtggatca tctggtcgag cgtgaccgtc aacgtgtcgc
    12721 gctcgccgag caagacgttg cccagcgaat cgccgacgag caatgcatcg accccgccgg
    12781 cctccgcggc acgcgccgtc ggcaggtcgt acgccgtcag catgacgagc ggcgggccga
    12841 ttctcttacg tttgacgaag cgcggaacgg tcatcacgcg ccggggttgg gactggaacg
    12901 tagacattgg cggcctccgc ggactccccg ccgaaaactc ggtcgaggga cctgtcagcc
    12961 tcggctcgtc acgcggacga gcccagtggt cggtagtact gtaccccacg gtccattttt
    13021 ctgatctgct ccaccagttc gtccaggtgc tctccgtgat gcacgaagtc gatcgatgtg
    13081 gtgtcgatca ccagcagcgg cgtctgcgcg tagtgaaaga agaagtagtt gtaggctttg
    13141 ttgacctcgt tgatgtacgt ttcggaaatc tgcgcctcgt aatctcggtt gcgtcgcgcg
    13201 atgcgctcga ccagcgttcg ggtctcggcc tgcaggaaga tgacgagatc cggcttgggc
    13261 acctggtctt cgagcatcgc gtagagcttg tcatagatca gcaactcgct gtcgtcaagg
    13321 ttgaggtagg cgaagatctt gtctttgggg aaaatgtagt cgcaaatcgt gacctggtgg
    13381 aacagatcgc gctgggagag ctcctgcagt tggcggtagc gcagcagcag gaagaacagc
    13441 tggacctgga acgccgcgcc cggttcgtcc tcgtagaagt tcttcaggaa cggattgtcg
    13501 agattctcca gcacgacctg ggcgtcgaat tttttcgcca gcaagttgac gaacgacgtc
    13561 ttacccaccc cgatcggtcc ttcaaccgcg atcgtactga acttcattga ccggagagtc
    13621 tcctgcaggt gggcgcgatt ataagggcag ccttccgcac gaggcaagag cgccgagccg
    13681 ctgcgctcgt gggttgcgcg gcaacgcatc gagttcttcg gcgacgctcc taccgctggc
    13741 ctcgttgacc aagtccggtg cgatctccgc cagcggcccg agcacgaatc gacgccgcca
    13801 catgcgcgga tggggtagcg acagccggtc atgctcgagt tcgatgccgc cgagccagag
    13861 caggtcgaga tcgatcgggc gcggcgcgtt ccgctcggtt cgctcgcgac cggcctgccg
    13921 ctcgatcgat agcaacgtat cgagcagctc gagtggcggt cgatcggtac gcacctcgac
    13981 ggccatgttc cagaaccagc gcggcgacgg cgagtcgacg ggctcggtct cccagaccgt
    14041 cgagctcgcg tacggcggca gaccgctggc cgacaggcga cgaagcgcgg cggcgaggtt
    14101 cgcgccccga tcgccgagat tcgagcccaa tgcgatgtag ccgatcacgc ggcggatgat
    14161 agcagcggag ccaggccgtt ctgcgcggcg gccggttgaa acctcccggc ggcgtcgcta
    14221 tcatgagcac tcttctggcg aaagccgggc aaatccggcg cttacccggt gatgccgacc
    14281 tcgcatcggg ctcggaggct tccgtatcat gacgaccacc gacaagaaac cggcggacaa
    14341 cgcatcgcac caccaacagt acgaagaggc gctgaaccgg ttcggcgtcg cgatcggcct
    14401 gctgcagagc ggcgccgtcg atgaagcgcg gcaggcgttc atcgagctcg aacgagcgaa
    14461 cgccgaggaa ccggcgctgg ccgaccgcgc gcgcagctac cgcaagatct gcgagcgcag
    14521 gctggccccg ccgcctgcgc cgccgcaaga cgccgacggg ttgtacgtcg ccggtgtgat
    14581 cgcgtccaac aacggcgatt acgacgaggc actggcgctg ctcgagcgtg cgctccggca
    14641 gacaccggac tcgccgaagg tgctctatgc gcgcgcgtcg acctggtcgc tgaagggcaa
    14701 cgccgaggcg gccgtggccg aattgcggcg cgcgatcgcc gtcgatccga gggtgcgatt
    14761 ccaggccgcc aacgaccccg acttcgagcc gatccgcgaa gaacccgcct tcatcgacat
    14821 catcgagccg acacccagcg gagcgtaacg catggctgcc accggggggg ggctcagagt
    14881 actggtcctg gcggccggtc gtggcaagcg cctgcgctcg aagtcgatca agctcgtcca
    14941 ttcggtggcc gggagtccga tggtcgctca cgtgctcgac gcggtggctc gcctgcggcc
    15001 gcaacggatc gtcaccgtca tcggcttcca ggccgatgag gtccgggcgg cgctcgacgg
    15061 ccaccgcagc gctttcgtcg tccagcgcga gcaacgcggc accggtcacg ccgtgctgca
    15121 ggcggcgcgg gcggtcggtg cttcgggcga cggcccgctg ttgatcgtca acggggacat
    15181 gccgacgatc cgcccatcga cgcttcgtcg attcgtctcg caccaccggc ggagccgggc
    15241 cgcgctcagc ttcatgacgg ccgagctcga cgaaccgacg ggttacgggc gcgtcgtccg
    15301 cgacggcgga gggcgggttg ttcgcgtcgt cgaacaagcg gacgccaccg ccgcggagaa
    15361 acggattcgc gaggtcaatt gcggaatcta ctgcgcgtca ccggctacgc tgtttcgcac
    15421 gctgcggcgg ctgactcccg acaacgccca gggcgaatac tacctcaccg acgcggtgca
    15481 cgtgctgctc gaagacggcg gcgcggtggc cgccgtccga cacgacgacg cggaggagct
    15541 gctcggggtc aatacacggc aggagttgtc ccgcgcttca cgcacgctgt acgcacgcaa
    15601 ggccgaagcg ctccaagacc agggcgtgac gttgctcgac gccgagcgca gctggatcga
    15661 cccgcgtgcc cgcatcggac gcgacacagt catctacccg ggcgtgctga tcgagggcga
    15721 aacggtgatc ggtcgcgact gcgtcatcgg ccttggctgc cggatcgcgg attcgacgat
    15781 cggcgacgac gtcgccgtgc gcgattacag cctgctcgag cgggcccggg tcgggacgcg
    15841 ggccgtcgtc gggccgttcg cgcggctgcg gcccgattcg tggctcgacc ccgacagtcg
    15901 cgtcggcaac ttcgtcgagc tgaagaagac gaggctcggt cgcgggagca aggcgaacca
    15961 cctggcctac ctcggcgatg cgacgatcgg tccggactgc aacgtcggcg ccggcaccat
    16021 cacctgcaac tacgacggaa ggaacaagtt ccccaccgtc ctcggacggg gggtgttcat
    16081 cggcagcgac acgcaactgg tcgcgccggt gcggctgggc gacgaggcgt acgtcggcgc
    16141 cggttcgacg gtaaccgagg acgtaccggc cggggcgttg gcgatcagcc gccaaccgca
    16201 gaccaacgtc gagggctggg tcgggcgacg caaacggcgg gacagggcgc gcagcaccaa
    16261 aaagtagccg gtcgcgccct ggcgtggaat cggcgcacgt gcacgccgta tattgacgct
    16321 gccgaacggg gtgtgcgcga acgatccgag tttgtcggct ggagatcctg gcatgtgcgg
    16381 aatcgtcggg tatatcggtg ccaagaaccc ggtcgaggtg ctcgtcgaag ggctgcggcg
    16441 tctcgagtac cgtggatacg actcggccgg tgtcgcggtg gtcgaccgcg ccggcgtgtt
    16501 gggcatccgc cgcgccccgg gaaagctgcg cgacctcgaa cgggtgctgg cggccgaccc
    16561 gctcgacggc gtctacggca tcggtcacac ccgctgggcc acgcacgggc gcccgtccga
    16621 ggagaacgcc cacccgcacc gcgactgttc cggccggctg gtcgtcattc ataacgggat
    16681 catcgagaac tacgtcgagc tgaagcacga tctcgagcga cgggggcacc ggttcgtgac
    16741 cgagaccgac accgagatcg tggcgcacgc gctcgaggaa gagctgaaga acggcgcgga
    16801 cgacttggtc gtcgcattgc gtcgagtgct acccacgttg cgcgggatct acgcgctggt
    16861 cgcgatgtcc gccgatcaac cccagaccgt cgtcggtgcg cgtcaaggcc ccccgctggt
    16921 cgtcggcatc ggcaagggcg agttctttct cgcgtcggac atccccgcga tcctgaccca
    16981 caccaaagac gtcgtgttca tggacgacgg cgaagtggtc gtgctgcgtc cgtcgggagc
    17041 gacctttctc gacgccgagg gtcgttcggt cgagcgaccg gtgacacggg ttccgtggga
    17101 tccgatcatg gccgagaaag gcggctacaa gcatttcatg ctcaaggaga tccacgagca
    17161 gccgcgcgcg gtgcgcgata cgctgctcgg tcgtgtctcg ctggagtcgg cggcgatcca
    17221 cctcgacgag atcgggctga gcgacgacta cctgcgcaag gtccaacgga tggcgatcgt
    17281 cgcctgcggc acatcgtggc acgccggact ggtcggcaag ttctggatcg agcatctggc
    17341 cggtctcccg gtcgaggtcg attacgcctc cgagttccgc tatcgttcgc cgctggtcga
    17401 caagctgacg ctgccgctct acatcagcca gtccggcgag acggccgaca cgctcgccgc
    17461 actgcgcgag gccaagcggc gcggcgccga gacgctcgcc atctgcaacg tccagggctc
    17521 gatggtcaca cgggaggcgt cgggcacgat ctacacccat gccgggcctg agatcggcgt
    17581 cgcctcgacg aaggcattca cgagccagct cgtcgcggtg atcctgctcg cgctgaagct
    17641 gggcagtgcg cgcgggcgtc tggacgagcg atcgatgctc gagatcgtgc gggcgctgta
    17701 ccacatcccg gcccagatgg agcagcacct cggcgacgac tcggcgatcg tcgaattggc
    17761 gaagacgttc cacaaccgtc gcgactttct ctacctcggc cgcggcgtga actacccgat
    17821 cgcgctcgag ggcgcgctca agctcaagga gatcagctac atccacgccg agggttaccc
    17881 ggccggcgag atgaagcacg gaccgatcgc gctgatcgac gaggaaatgc cggtggtcgc
    17941 cgttgcccct cacgattcgg tctacgagaa gatgctgtcc aacatcgaag aggtcaaagc
    18001 ccgctccggt atcgtcctgg ccgtgaccga ccgcgacgac gcggacctgg cgcacaaggt
    18061 cgacgcagtg atccgcgtgc cgcggaccca cgagctgctg tcaccgctgc tgaccgcgct
    18121 gccgatgcag ctgttggcgt atcacatcgc gctgctccgc ggttgcgatg tcgatcaacc
    18181 gcgcaacctg gcgaagagcg ttaccgtcga gtaacgcggg ccgcggtcgg ttcgcggctt
    18241 ttccgtgccg gagttggtac aatgcggtct ttgtccacgt gggaggccgc gtgattcccc
    18301 cggagcaccc aggtttggcg
//
LOCUS       DASPGN010000034         2905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1294955, whole genome shotgun sequence.
ACCESSION   DASPGN010000034 DASPGN010000000
VERSION     DASPGN010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294955"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VD788_01055"
     CDS             complement(<1..208)
                     /locus_tag="VD788_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74878.1"
                     /translation="MLAKLASLRLALPMLTTLLVITIGAGVSGVASSLLRRLGDEQAI
                     ERVRTAGLAAERELDQVGRDVEVGV"
     gene            complement(210..1079)
                     /gene="cysW"
                     /locus_tag="VD788_01060"
     CDS             complement(210..1079)
                     /gene="cysW"
                     /locus_tag="VD788_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HXV74879.1"
                     /translation="MKEVTLASRGKPREPRAPSATEPLWVRSLLVAVALGFLLLFLVL
                     PLSLVFAKAFADGGAAWAAAVTDPVARASLRLTLLTAAIVLPVNVVFGVAAAWATARF
                     RFRGRNLILTLIDLPFSVSPVVSGMVFVLLFGASGWFGPWLQQHGIQIVFAVPGIVLA
                     TLFVTAPFVARELVPSLEAQGIVEEEAALTLGAGGFRTFVSISLPKVRWALLYGIVLC
                     TARAIGEFGAVSVVSGHIRGRTTTVPLHVEMLYNEYQFTAAFAVASMLAVLALVTLML
                     KYLIERRSVQGRR"
     gene            complement(1076..1894)
                     /gene="cysT"
                     /locus_tag="VD788_01065"
     CDS             complement(1076..1894)
                     /gene="cysT"
                     /locus_tag="VD788_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HXV74880.1"
                     /translation="MAARGRVLPGFGLALGITLTYLGLVILIPLSTIPLKTATMGWDR
                     FVEVVSSPRALAAYRLSFGAALAAAAINAVFGFLVAWVLERYSFPGRRLIDGLVDLPF
                     ALPTAVAGIALTTLYAPGGWIGRWLEPAGLKVAYTPLGVVVALTFIGLPFVVRSVQPV
                     LGALGAELEEAAASLGAGRLAIFRRILLPRLVPAIVTGSTLAFARGLGEYGSVIFIAG
                     NLPLESEITPLLIVIRLEEFDYPAATAIAFVFLVMSFVALLSIHLLSHWRGRRS"
     gene            complement(1919..>2905)
                     /locus_tag="VD788_01070"
     CDS             complement(1919..>2905)
                     /locus_tag="VD788_01070"
                     /inference="COORDINATES: protein motif:HMM:NF008106.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXV74881.1"
                     /translation="IVACVAATLAGAATAADLTLLNVSYDPTREFYQEVNQAFAEHWR
                     ATTGRSVSIEQSHGGSGKQARSVLDGLAADVVTLALGADIDAIASNGMIARDWQRRLP
                     GNSCPYTSTIVFLVRQGNPKKVRDWDDLAKPGVQVITPNPKTSGGARWNYLAAWGWAL
                     ARPGGTEAGARALLAAIYKNVPILDTGARGSTVTFVERGIGDVLIAWENEALLATRKF
                     EKGKFEIVVPTISIRAEPPVTWVDTVVRRKGTADLARAYLEFLYTPEIQQAAARHFYR
                     PYDETVAERFKERFPVVEMFTIDEVFGGWTNAQRVHFADGGEFDRLYRGAAR"
BASE COUNT          508 a         1025 c          983 g          389 t
ORIGIN      
        1 gcactccgac ctcgacgtcg cgcccgacct gatcgagctc ccgttctgcg gcgaggcccg
       61 ccgtgcggac ccgttcgatc gcctgctcgt cgcccaggcg tcgcagcagc gagctcgcca
      121 ctccgctgac gcccgcgccg atcgtgatga ccaggagcgt cgtcagcatc ggcagcgcga
      181 gccgcagact tgccagcttg gccagcacgt cagcgacgtc cctgcaccga ccggcgctcg
      241 atcaggtact tgagcatcag cgtcaccagc gcgagcacgg cgagcatcga ggccaccgcg
      301 aaggctgcgg tgaactggta ctcgttgtac agcatttcga cgtggagcgg gaccgtcgtc
      361 gttcgcccgc ggatgtggcc ggagacgacg gacaccgcac cgaactcccc gatcgcccgt
      421 gcggtacaca gcacgatacc gtacagcaac gcccagcgga ccttcggcag gctgatcgag
      481 acgaacgtcc tgaatccgcc ggcgccgagg gtcaacgcgg cctcctcctc gacgatgccc
      541 tgcgcctcga ggctgggtac cagttcgcgg gcgacgaacg gcgcggtcac gaacaacgtg
      601 gccaggacga tcccggggac ggcgaacacg atctggatcc cgtgctgctg cagccacggc
      661 ccgaaccagc cgctggcgcc gaacagcagg acgaacacca tgccggagac gaccggggaa
      721 acgctgaacg gcaggtcgat cagcgtcagg atcagattcc ggccgcggaa gcggaaccgg
      781 gccgtggccc atgccgcggc gaccccgaag acgacgttga ccggcagcac gatcgccgcg
      841 gtcaacagcg tcaaccgcag cgaggcgcgc gccaccggat cggtgacggc ggcggcccag
      901 gcggccccgc cgtccgcgaa cgccttggcg aagacgaggg agagcggcag gaccaggaac
      961 agcagtagga agccgagcgc gacggcgacg agcagcgagc ggacccacag cggctcggtg
     1021 gcggacggcg cgcgcggctc ccgcggcttc ccgcgactgg cgagcgtgac ctccttcatg
     1081 atctccggcc tcgccaatgg ctcagcagat ggatcgacag cagcgcgacg aacgacatga
     1141 cgaggaaaac gaacgcgatc gcggtcgccg cgggataatc gaactcctcg agccggatca
     1201 cgatcaaaag cggtgtgatc tccgactcga gcggcagatt gccggcgatg aagatgaccg
     1261 acccgtactc gccgaggccg cgggcgaagg ccagggtcga gccggtgacg atcgccggta
     1321 cgagtcgcgg cagcaggatg cgccggaaga tcgccaagcg gccagcaccg agactggctg
     1381 cggcctcttc gagctccgct ccgagcgctc ccagcaccgg ctggaccgag cggacgacga
     1441 acggcaaacc gatgaaggtc agcgcgacga cgaccccgag cggcgtgtag gcgaccttca
     1501 gcccggcggg ctcgagccag cggccgatcc agcctcccgg agcgtacagc gtcgtcagcg
     1561 cgatgccggc gaccgccgtt ggcaacgcga acggcaggtc gaccagcccg tcgatcagcc
     1621 ggcgcccggg gaacgagtag cgttcgagca cccaggcgac gaggaagccg aagaccgcgt
     1681 tgatcgccgc cgctgcgagc gcggcaccga agctcagccg gtaggcggcg agtgcccgag
     1741 gcgacgaaac gacctcgacg aaccggtccc agcccatcgt ggccgtcttg agcgggatcg
     1801 tgctgagcgg gatcaggatc accagtccca ggtaggtcag cgtgatcccc agtgccagtc
     1861 cgaagccggg aagcacgcgc cctcgcgccg ccatttcgtc gcctcgaccc cgccgcggct
     1921 agcgcgcggc gcctcggtac agtctgtcga actcgccgcc gtcggcgaag tgcactctct
     1981 gcgcgttggt ccagccgccg aacacctcgt ctatcgtgaa catctcgacg accgggaacc
     2041 gttccttgaa gcgctcggcg acggtctcgt cgtacggccg gtagaagtgg cgggccgccg
     2101 cctgctggat ctcgggggtg tagaggaact cgaggtacgc gcgcgcgagg tcggcggtgc
     2161 ccttccgccg gactaccgta tcgacccacg tcaccggcgg ttcggcccgg atgctgatcg
     2221 tcggaacgac gatctcgaac ttccccttct cgaacttccg cgtcgcgagc agcgcctcgt
     2281 tctcccacgc gatcagcacg tcgccgatcc cgcgctcgac gaacgtcacc gtcgatccgc
     2341 gtgcgccggt atcgaggatc gggacgttct tgtagatcgc cgcgagcagc gcccgcgcgc
     2401 cggcctcggt cccgcccggc cgcgcgagcg cccagcccca ggcggcgagg tagttccagc
     2461 gcgcgccgcc cgatgtcttc gggttgggcg tgatcacctg gacgcccggt ttggccaggt
     2521 cgtcccaatc gcggaccttc ttcggattgc cctggcgaac gagaaagacg atggtcgagg
     2581 tgtacgggca cgagtttccc ggcaatcgtc gctgccagtc ccgcgcgatc atgccgttgg
     2641 acgcgatcgc gtcgatgtcg gcccccaggg ccagcgtgac gacgtcggcc gcgagtccgt
     2701 ccaagaccga tcgggcctgc ttgcccgacc caccgtgcga ttgctcgatg ctcacggatc
     2761 gcccggtcgt ggcccgccag tgctcggcga acgcctgatt cacctcctgg tagaactcgc
     2821 gcgtcggatc gtaggacacg ttcagcagcg tgaggtccgc cgcggtcgcc gcgccggcga
     2881 gcgtcgcggc gacacacgcc acgat
//
LOCUS       DASPGN010000035        16638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1529177, whole genome shotgun sequence.
ACCESSION   DASPGN010000035 DASPGN010000000
VERSION     DASPGN010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 16638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16638
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529177"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VD788_01075"
     CDS             <1..811
                     /locus_tag="VD788_01075"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HXV74882.1"
                     /translation="AAALPDAPPHPSGSFPLVIDYALAKGLAHEAFGHAAEADGYRSS
                     VLARDGRFRVGETVGPEHVSIVDEPISGDHAFQPYSANAVTRERAVIVDRGVLVDGLS
                     DPWSAEAGGVRLTGAARAESFHDAPQPRMTNIRIETREPLPAPGEFEAYGPQEVRELL
                     RMAGLFARHPRWVFLSGYSGGQVNTTSGDFVFNCKAIYDLDDRGVALFKPAIFSGSMF
                     GALQSVREAFGPLKLDALGYCGKWGQSVASSGGSHYFLALEPDPSVRLGGR"
     gene            808..2127
                     /locus_tag="VD788_01080"
     CDS             808..2127
                     /locus_tag="VD788_01080"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HXV74883.1"
                     /translation="MKLDPLLDALSGRFAGGRAVRAWRLYAADGRRVSLGVKDRDYGG
                     PHAPLALARSGGVRYLLVWDDGRVSRGYLERCHFEESPSRALAEALRAAYDDPDAAQV
                     LGPTPLPEVELHDPATAAAVDGDLSLLARRLDLVRERTAGDRVDTWSGSFSFGEGGFR
                     LLTSAGFDESGCGTSFGWHVTLNGEASDGFSARAPEIDAAFEARLGRLLARAGELSRP
                     AATIAAGIRPVVLAPRVVESYVLETLLANLDGSTVSNGESHFRREQFGSGEPILRDDM
                     ELRIDPLQPLRSGAYRCTREGVPAARCSYITNGCLEHPVAGLKYARRLGIRPTPVPYA
                     SDTLFFSGRRSLTETESLERAAGGALILSVLGVHTQDAASGDFSLSAPQALGLTPAGF
                     AGRLKATISGNLFDLLSDPATEFVRFDGEHTPGLLVSCRLDPSEPTA"
     gene            2222..4837
                     /gene="clpB"
                     /locus_tag="VD788_01085"
     CDS             2222..4837
                     /gene="clpB"
                     /locus_tag="VD788_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HXV74884.1"
                     /translation="MQFDKLTIKLQQALQQSQRLAAERGHAEVRPLHLLHELTVQEHG
                     VVVPILQRLGADPQALGSAVVQRLGKIARVAGGTQPGPSRSLTETLDSAERIAEEFQD
                     DYVSTEHALLAMARSDDETAELLVQFGATPDGVLAALRHVRGTARVTDANPEEKYQAL
                     KRYARDLTALARNGKLDPVIGRDDEIRRTMQVLCRRTKNNPVLIGDPGVGKTAIVEGL
                     ARRIADGDVPETLKDRSLVALDLGALIAGSKYRGEFEDRLKAVLKEIEASDGRIMLFI
                     DELHTLVGAGAAEGAVDAANLLKPALARGQLRCIGATTVQEYRKHIEKDAALERRFQP
                     VQVGEPSVEDTISILRGLRERYEVHHGVRIQDAALVSAAVLSDRYITDRFLPDKAIDL
                     IDEAAAQLRMQIDSVPEEIDEVQRRVLQLEVEKQALKRERDAASKDRAKKIDKELADL
                     VERLGAMKQRWANEKSVISRLSEINQRLERCKLEAERAEREADLQRAAELRYGELPKL
                     QAEQEREQRRLSELQQAGALLHEEVGEQEIATIVSKWSGVPVAKLLEGEVDRLVHLED
                     QLHARVVGQQAAVAAVATAVRRARAGLKDPDRPVGSFLFLGPTGVGKTELAKALAVSL
                     FADEKAMVRLDMSEYSERHAVARMIGAPPGYVGYDEGGQLTEAVRRRPYSVILLDEIE
                     KAHPEVFNVLLQVLDDGRLTDGKGRTVDFRNAILIMTSNIASQYILEHAGESTERLQR
                     HVDLELHRIFRPEFLNRLDETIVFSSLSKDELLSIVDLQLAQLNRTVADRRLKIVADE
                     GAKRRLAELGYDPAYGARPLRRAIQKRILDPLAGGLLEGRFAAGDTIRVVADGTELTL
                     RREAAAPAATDPVSA"
     gene            4862..5317
                     /locus_tag="VD788_01090"
     CDS             4862..5317
                     /locus_tag="VD788_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXV74885.1"
                     /translation="MVIQRWDPLRDLMELQDRMKQLFADALSRSAGTVGAGEGGGNGW
                     VPPIDLFEEEERYVLRADLPGVSPEAVEIQIENGLLTLGGERKVDSTVSPDSFLRIER
                     PYGRFAAQLSLPPSVETRSIRAAHRNGVIEIVLPKRKDAPPSRIEVAAD"
     gene            5402..6247
                     /locus_tag="VD788_01095"
     CDS             5402..6247
                     /locus_tag="VD788_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HXV74886.1"
                     /translation="MNTSNMIRTTLLLGLMTGLFLAIGYALGGQAGLVIAFALAAVMN
                     FGSYWFSDKIVLASHRAQPVDARQAPRLHAIVDRLVARAGLPKPRLYVLPERAPNAFA
                     TGRDPEHAAVAVTQGLLELMDEEEVEGVVAHELAHVKNRDILISSIAATLAGAVTMLA
                     TMARWGAIFGGGGRDDDNRGGLIGLIAMSILAPIAALVIQMAVSRSREYAADATGARM
                     IGHPHGLARALGKLDQYSKRIPLHSSPASSHMFIVMPLSGRAVMSLFSTHPPIEQRIR
                     RLLGR"
     gene            6286..6918
                     /locus_tag="VD788_01100"
     CDS             6286..6918
                     /locus_tag="VD788_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXV74887.1"
                     /translation="MKDEQPTEKPPAAKGNGEERDGAFRFEDRRRWAQGSETEPNEAQ
                     TSPRTPTLIDEYRERAESAEQKLQEYIEAFKEFRREQDSFRERLGRDVDRRVELKFGS
                     LVHDLLEILDNLDRALEHVQDVPEAAELARGVELARNSFLETLERNGVERVEPHGAEF
                     DPNESEAVRLDAVDDPRLSGKVTEVVRPGYRLGERIVRPARVAVGRHDGP"
     gene            complement(6925..8316)
                     /locus_tag="VD788_01105"
     CDS             complement(6925..8316)
                     /locus_tag="VD788_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HXV74888.1"
                     /translation="MDERAGELTRKSIHVAFGGLALALRWLEPWQAAAGAFAAALFNL
                     LALHRLTRGALLRAGERAGGFSWGVAGYPCAVLALIVVFRERLELAAAVWGLVAFGDG
                     MAAITGVVVGGPRLAWNRRKTVVGSLAFIAWGTAGSALLLRWTQLAALDGDPGGRIGA
                     SFLATGVADERWLLGGCLVAAIAAAGVESLDSGVDDNLTVPLVGGAVLSVMSVVDPGR
                     WPEIVRLAAERLPLGFPLLAAAAGAAFSLRIVTVGGAIAGLGLAAILLAALGWGSLTI
                     FALVVVIAAIATRLGRARKEGAGLAEGSAGRRGAAQALANTSAAAIFAILAAVTPSDP
                     VFRIALTASLATALADTVATEIGQAYARRAWALVPPKPVPLGTPGAVSSVGTLAAAAA
                     AATIAATGVVCDMTPLAASAAVAAGAWLAAMAESLVGGLWRSARPADHGLLNFANTLC
                     GAGAAVALYQLGR"
     gene            complement(8309..9181)
                     /gene="rfbD"
                     /locus_tag="VD788_01110"
     CDS             complement(8309..9181)
                     /gene="rfbD"
                     /locus_tag="VD788_01110"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HXV74889.1"
                     /translation="MVEPEPILVVGGSGLLGEALQSELAARGRRSSAPTRRQLDLTDP
                     RRVDAWLDRHRPAAVINAAAYSDVTRAERPDQCEQVFLLNRDAPADLALRCRRRGIAF
                     VYISTDFVFDGTANRPYVEGDEPRPLQVYGRSKLAGERAVSRAHPESLIVRTSTLFGP
                     GRRPRPHYVDAVLAQARKGGRVEVVRQPVSSPTFTSDLAAALVELVDRGSTGVLHLVN
                     DGACSRLELARAAVRIVGFAERVEVVERPTPDDGPQRPAYSVLDTTELAARLGRRLRS
                     WDEALAAYLAPRDG"
     gene            complement(9221..11611)
                     /gene="lptD"
                     /locus_tag="VD788_01115"
     CDS             complement(9221..11611)
                     /gene="lptD"
                     /locus_tag="VD788_01115"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HXV74890.1"
                     /translation="MILFPSPTTIRIRALLWVVLLTVPSLATSQSDDRATPPEREPET
                     LPRNEMLKDLEIQVWGPWRQTAERDYRFDGKVTIIWREQQIQADRLTLTDGRYIEATG
                     NILVVWEGNRISGARLTYDLDAERGMIEKAIGQVQGDFLFWAKRAEKIGDRTVKLESA
                     TVTTCTQPVPYWSFSVTSATVTIERYARMWNVVARASSVPFFYSPYLIWPVKQDRSLG
                     LLLPEFGTNEKLGQTISLPLFIPIGRSADVTLFGEYFTDAGFGMGGDFRMIPNRRGAA
                     RLQGFYIDDQVSTDTTGELIGDRYNLVYQQTQEFTNGFRMTADINAVSDFNYYTDYER
                     ELNLISTPQILQRVEFSRNSSWTSINARELRREQLFADGSTLLQTTLPELEFRGRSNR
                     LGKSPVYLQYEASAASILQREDDVSGRPPFRAEYLRGDLFPTVSLPWSPTPWLDVTPR
                     AFYRFTYYTERQTMTSNPFNPAGPSVRSTLDQDLIRNLWGANLLLVGPKLARIYGQPN
                     RRQFKHAIEGRVTYGYEDSFARADEIIRFDEVDVFNGAGNAMRYSIAQRLFVKRPRAA
                     ETIVIDSNEAIVLPDGTVSSTDPSAPPAPEPSDAPPPPNEAVEVANLEIGQSRSFDQD
                     LSTADLDRDGVVEATSPYSSVGITARYNPDATTTVDLRGDYDILWKQFSGATLSGGIR
                     RQLAQVRFSLVHRNGLGVDAATLAPNTDDTQFRLTTGLSFWRGKFRLLLDGTVDFDPQ
                     PGQDTVPSRAWRLEYSTQCCTVYLESLDRTYTGSEREDFRFRVDLKGIGKILQVTY"
     gene            complement(11694..12671)
                     /locus_tag="VD788_01120"
     CDS             complement(11694..12671)
                     /locus_tag="VD788_01120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV74891.1"
                     /translation="MPRLELASRAQIDGIARESYLLWGGGLSLADYRELWDEVGATPW
                     CRDHAAYYVWRDGRGRALSSMKVYRPALRLGDRVGRASVFGAVFTPTVFRRRGFASAM
                     LAAGVERSREQGDLAALLFSDIGTRFYADLGFRALPAPEYLGRLTPGEPAVEEIGLRP
                     VLASELELFQRAHAASTRSRPIAILRDAAHWKFLWVRSRAFFGRVRGAGIRHEWRIAS
                     RCGRFAGYVIAVEGRGEWNVREVASVDGDPRGMAAILMRAADDAYRRGCRRVYGWLPP
                     DLADQLGAWSLDRRERRRAVPMLRPGPVTVDPEPLLGAPANHIPFQDQF"
     gene            complement(12771..12992)
                     /locus_tag="VD788_01125"
     CDS             complement(12771..12992)
                     /locus_tag="VD788_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74892.1"
                     /translation="MNIFELRRAYHNSLSNMRGWMGDDSLSGRLTVLDRLSILDAWQQ
                     EMVEFFEKNGHCFVCNRRLERCRCPKVAS"
     gene            complement(13323..14498)
                     /locus_tag="VD788_01130"
     CDS             complement(13323..14498)
                     /locus_tag="VD788_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV74893.1"
                     /translation="MTRKADYRVIESEETAEVIVRQSPAEIESVQPTIAAHQRAVEPG
                     KPTAAILPIYLREMGATPLIDENQEVDLARELQDAREALAKIALKLPAAAKAYCLEGD
                     VDGPKRGREWPLDDMETFYRKLLRYGRESGKEARTLALVSQAKRQKHHVNHARDALIL
                     ANLRLVVHIAKKYLNHGISFMDLIQEGNIGLMKAVEKFEYERGNKFSTYAYWWIKQAI
                     ERAIADKARVIRIPVHVNEKIKKISRISKELAETLGRRPTPPEIARKLRMPIAKVEEI
                     LGVVPEPQALEDMSSEDDSPGLLRFIADPNAPSPLERTVDRELREKVEGTLKVLTSRE
                     EEIIRLRFGIGREMPYTLEEIGRVMGLSRERVRQIEATALKKIQAAEESRDLREFLG"
     gene            14849..15736
                     /locus_tag="VD788_01135"
     CDS             14849..15736
                     /locus_tag="VD788_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXV74894.1"
                     /translation="MDDLKVSIAQIAPKLGDVEANVELHLQAIDAARDAASDLVVFPE
                     LSLTGYHLGDQVPELALGDDSAPLRAIADASRAIDVVFGYVEDAPGHRFFNAAAYLSA
                     GEPVHVHRKVYLPTYGMFQEGREFAAGARLRAFDTRHGRLGLLICEDLWHPTSGWLLT
                     QQGAEVVITVGNGPTRGTRPGDGITSVGVWRDLVRTNAQFGTSFFLFVNRVGCEDGLT
                     FGGGSTVADPFGRIVLELPPLEPALGRVELTSDVVRRARATYPLLRDSDLELVDRELK
                     RIRSARYGITIEDRREPHE"
     gene            15729..16580
                     /locus_tag="VD788_01140"
     CDS             15729..16580
                     /locus_tag="VD788_01140"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXV74895.1"
                     /translation="MSDGTIPEAVDGDLAIDPDWTTRILARFVASEVGRTGKRSVVVG
                     LSGGIDSAVSAALAARALGPARVHCLLMPHRDSNPHSERDARRFAEQLGSPSETVDIS
                     PLVDGFVAVSGEVGRLRLGNVMARARMIVLFDRSAALDALVLGTSNKTELLLGYGTVH
                     GDMASAVNPIGDLYKTQIRALAAHLDVPEAIRNKPPSADLWPDQSDEEDLGFRYDEVD
                     RLLALLVDARIDRREAVRRGFAPGFVDGVIRQIVRSQFKRLPPVIAKVSSRSIGWDFR
                     YPRDWLS"
BASE COUNT         2746 a         5623 c         5639 g         2630 t
ORIGIN      
        1 cgccgcggcg ctgccggacg cgccgccgca tccatcgggc agcttcccgc tcgtcatcga
       61 ctacgcgttg gccaaggggc tggcgcacga agcgttcggc cacgccgcgg aggccgacgg
      121 ctatcgctcg tcggtactgg cgcgcgacgg ccgcttccgc gtcggcgaga cggtcggccc
      181 agagcacgtg tcgatcgtcg acgagccgat cagcggagac catgcgttcc aaccgtacag
      241 cgccaacgcc gtaacgcgcg agcgagcggt gatcgtcgat cgcggcgtgc tggtcgacgg
      301 gctgtccgat ccatggtcgg ccgaggcggg tggcgtgcgc ctgaccggcg cggcccgcgc
      361 cgagtcgttc cacgacgcgc cgcaaccacg gatgaccaat atccgcatcg agacccgcga
      421 accgctgccc gctcccggcg agttcgaggc ctacggaccg caagaggtgc gagagttgct
      481 ccgaatggcg gggttgttcg cgcgccaccc ccgttgggta ttcctttccg gttacagcgg
      541 cgggcaggtc aacacgacgt ccggggactt cgtcttcaat tgcaaggcga tctacgacct
      601 cgacgatcgg ggagtcgcgt tgttcaagcc ggcgatattc tccggatcga tgttcggcgc
      661 gttgcagtcg gtgcgcgagg ccttcggtcc gctcaagctc gacgcactcg gctactgtgg
      721 taagtggggc cagagcgtcg cgtcgagcgg agggagccac tacttcctcg cgctcgaacc
      781 cgatccgtcg gttcgactcg gcggccgatg aagctcgatc ccctgctcga cgcgctctcg
      841 gggcgtttcg ccggcggccg cgccgtccgc gcctggcggt tgtacgccgc cgacggccgg
      901 cgcgtctcgc tcggcgtcaa ggaccgcgat tacggcgggc cgcacgcgcc gctcgcgctc
      961 gcccgctccg gtggcgtgcg ctacttgctg gtttgggatg acggccgggt cagccggggc
     1021 tacttggagc gctgccattt cgaggaatcc ccgtcgcgtg cgctggcgga agcgctgcga
     1081 gcggcctacg acgacccgga cgcggcgcag gtgctcggcc cgaccccgtt accggaagtc
     1141 gagttgcacg acccggcgac cgccgccgcc gtcgacggcg acctctccct gctcgcacgg
     1201 cgactcgacc tcgtgcgcga acggaccgcc ggcgatcgag tcgacacgtg gagcggttcg
     1261 ttcagcttcg gcgagggagg attccgcttg ttgacctcgg ccggtttcga cgagagcggg
     1321 tgcggaacct cgttcggttg gcacgtcacg ctcaacggcg aggcgagtga cggattctcg
     1381 gcccgtgctc cggagatcga tgccgcgttc gaggcgcgcc tcggccgttt gctcgcgcgc
     1441 gccggcgagc tgagccgccc cgccgccacg attgcggcag ggattcgccc ggtggtgctc
     1501 gcgccgcgcg tcgtcgagtc ctacgtcctc gagaccctgc tggccaacct cgacgggtcg
     1561 acggtgtcca atggggagag ccacttccgt cgcgagcagt tcggctcggg cgagccgatc
     1621 ctgcgcgacg acatggaact gcgaatcgac ccgctgcagc cgttgcgtag cggcgcctat
     1681 cgctgcacgc gcgaaggcgt gcctgcggcg cggtgcagct acatcaccaa cggttgcctc
     1741 gaacaccccg tcgccgggct gaagtacgcg cgccggctcg ggatccgccc gaccccggta
     1801 ccctatgcgt cggacacgct gttcttctcc ggccggcggt cactgacgga gaccgaatcg
     1861 ctcgaacgag ccgcgggcgg agcgttgatc ctgtcggtcc tcggcgtcca cacgcaggat
     1921 gccgcgagcg gtgacttctc gctttcggca ccgcaggcgt tggggctcac gccggccggc
     1981 ttcgccggcc gactcaaggc gacgatctcg ggcaacctgt tcgacctgct gtcggatccc
     2041 gcgaccgagt tcgtccggtt cgacggcgaa cacacgccgg ggctgctcgt ttcgtgccga
     2101 ctcgacccca gcgaaccgac cgcctaacac gtgcccgcgc aaggggtttc cccggttgcg
     2161 ccaccggcct tgacacaccc cggccggggt cccatgaacg ggtcgttcgg tggagtgtcg
     2221 catgcaattc gacaagctga cgatcaagct ccagcaagcc ctgcaacagt cccagcgtct
     2281 cgccgccgag cgcggtcacg ccgaggtgcg cccgctgcac ttgcttcacg agctgaccgt
     2341 gcaagagcac ggcgtcgtcg ttccgattct gcagcgactc ggcgccgatc cgcaagcgct
     2401 cggcagcgcg gtggtgcagc ggctggggaa gatcgcacgg gtcgcgggcg gcactcagcc
     2461 cggaccctcc cgctcactga ccgaaacgct cgacagcgcg gagcggatcg ccgaggaatt
     2521 ccaggacgac tacgtctcga ccgagcatgc cctgctggcg atggcccgaa gcgacgatga
     2581 gaccgcggag ctgctcgtcc agttcggtgc cacgccggac ggcgtgctgg cggcgctgcg
     2641 ccacgtgcgc ggaaccgccc gggtgaccga cgccaatccc gaggagaagt accaagcgct
     2701 gaaacgctat gcccgcgatc tgaccgcact ggcgaggaat ggcaagctcg acccggtcat
     2761 cggccgcgac gacgagatcc ggcggacgat gcaggtcctg tgtcggcgga cgaagaacaa
     2821 cccggtgctg atcggtgacc ccggggtggg caagaccgcg atcgtcgagg gcctggcacg
     2881 ccgcatcgcg gacggcgacg tacccgaaac gctcaaggac cgttcgctgg tcgccctcga
     2941 cctcggtgcg ttgatcgcgg gatcgaaata ccgcggcgag ttcgaggacc ggctgaaagc
     3001 ggtgctcaag gaaatcgagg cctccgacgg gcgcatcatg ttgttcatcg acgagctgca
     3061 cacgctggtc ggcgccgggg cggccgaggg cgcggtggac gccgcgaacc tgctgaaacc
     3121 cgcgttggcg cgtggccagc tgcgctgcat cggtgcgacg acggtccaag agtatcgcaa
     3181 gcacatcgaa aaggacgcgg cgctcgagcg gcgcttccag ccggtccagg tcggcgagcc
     3241 gagcgtcgag gatacgatct cgatcctccg cgggctgcgc gaacgttacg aggtgcacca
     3301 cggcgtgcgc attcaggacg ccgcgctcgt atcggccgcc gtgctgtccg accgttacat
     3361 caccgaccgc ttcctgcccg acaaggcgat cgacctgatc gacgaggcgg cggcgcagct
     3421 gcggatgcag atcgattcgg tgcccgagga gatcgacgag gtccagcggc gcgtgctgca
     3481 gctcgaggtc gaaaagcagg cgctcaaacg ggaacgcgac gcagcctcca aggaccgggc
     3541 gaagaagatc gacaaggagc tcgccgacct ggtcgaacgc ctcggcgcaa tgaagcagcg
     3601 gtgggcgaac gagaagagcg tgatctcacg gttgagcgag atcaaccagc gtctcgagcg
     3661 gtgcaagctc gaggcggagc gcgccgagcg cgaagccgac ctccaacgcg ccgccgagct
     3721 gcgctacggc gagttgccga agctgcaggc cgaacaggag cgcgagcaac gtcgcttgag
     3781 cgaactccag caagcgggcg cgctgttgca cgaggaagtc ggcgagcagg agatcgccac
     3841 gatcgtgtcg aagtggagcg gggtcccggt ggcgaagctg ctcgagggcg aggtcgacag
     3901 gttggtccac ctggaagacc agctccatgc ccgggtcgtc ggccagcaag cggccgtcgc
     3961 cgcggtggcg accgcagtgc gacgcgcgcg cgccggcctc aaggaccccg accggccggt
     4021 gggcagtttc ctgttcctcg gaccgaccgg ggtgggcaag accgagttgg ccaaagcgct
     4081 cgccgtcagc ttgttcgcgg acgagaaggc catggtgcgt ctcgacatgt ccgagtactc
     4141 ggagcgacac gctgtggcgc gcatgatcgg cgcgcctccg ggctacgtcg ggtacgacga
     4201 gggcggacaa ctgaccgagg ccgtgcgacg gcgtccgtac tcggtgatcc tgctcgacga
     4261 gatcgagaag gcgcacccgg aggttttcaa tgtcctgctt caggtgctcg acgacgggcg
     4321 gctgacggac ggcaagggac ggaccgtcga cttcaggaac gcgatcctga tcatgacgtc
     4381 gaacatcgcc tcgcagtaca tcctggaaca cgccggtgag agcaccgaac gactgcaacg
     4441 gcacgtcgat ctcgagttgc accggatctt ccgtcccgag tttctcaaca ggctcgacga
     4501 gacgatcgtc ttcagctcgc tgtccaaaga tgaactgctg tcgatcgtcg acctgcagtt
     4561 ggcacaattg aatcgaactg tggccgaccg ccggttgaag atcgtcgcgg acgagggcgc
     4621 caagcggagg cttgccgagc tgggctacga cccggcctac ggggcccggc cgttgaggcg
     4681 cgcgatccag aagcgaatcc tggatccgtt ggccggcggt ctgctcgaag ggcgcttcgc
     4741 cgcgggcgac accattcgcg tcgtcgccga cggaaccgag ctgaccctcc gccgtgaggc
     4801 cgcggccccc gccgcgaccg acccggtttc ggcgtagaat gtcgagcgga ggtagtgacc
     4861 gatggtgatt cagcgatggg atccgctgcg cgacctgatg gagctgcagg accggatgaa
     4921 gcagttgttc gccgacgcgc tgtcgcgctc ggccgggacg gtcggcgccg gtgaaggcgg
     4981 cggcaacggc tgggtcccgc cgatcgatct gttcgaggag gaggaacgtt acgtcctgcg
     5041 tgccgacttg ccgggtgtga gccccgaggc ggtcgagatt cagatcgaaa acgggctgct
     5101 gacgctcggc ggcgaacgca aggtcgactc gacggtgtcg ccggactcgt tcctgcggat
     5161 cgagcgtccc tacggacggt ttgccgccca gctgtctctt ccgccgtccg tcgagacccg
     5221 ctcgattcgc gcagcccatc gcaacggcgt gatcgagatc gtcctgccca aacgaaagga
     5281 cgctccgccc agccggatcg aagtggccgc cgactgaacg agggcgcggc ccggccgatc
     5341 gaccacgaga acaaccgacc agaccgccgg ctcgccgacg agcggcggtg ggagtgaagc
     5401 gatgaacacg agcaacatga tcaggaccac gctgctgctg gggctgatga ccggcctctt
     5461 tctcgcgatc ggctacgcgt tgggcggcca ggcggggctc gtcatcgcgt tcgcgctggc
     5521 cgcggtgatg aacttcggat cgtattggtt ttccgacaag atcgtgctcg ccagccaccg
     5581 tgcacagccg gtcgatgccc gacaggctcc acggctccac gcgatcgtcg accgcttggt
     5641 cgcgcgggcc ggtttgccga agccgcgtct gtacgtgctg cccgagcgcg ccccgaacgc
     5701 cttcgcgacc gggcgcgatc ccgaacacgc cgccgtcgcg gtgacgcagg gtctgctcga
     5761 gctgatggac gaggaggagg tcgagggggt cgtcgcgcac gagctggcgc acgtcaagaa
     5821 ccgcgacatc ctgatctcct cgatcgccgc gacgctggcc ggcgcagtga ccatgctggc
     5881 gacgatggcg cgctggggcg cgatcttcgg cggcgggggc cgggacgacg acaaccgggg
     5941 aggtctgatc gggctgatcg cgatgtcgat cctggcgccg atcgccgcgt tggtcatcca
     6001 gatggcggtt tcccgctcgc gcgagtacgc agccgacgcg accggcgcgc ggatgatcgg
     6061 ccacccccac ggtctggccc gcgcactggg caagctcgac caatactcca agcgtatccc
     6121 gctccactcc tccccggcga gtagccacat gttcatcgtc atgccgctgt cgggccgggc
     6181 ggtgatgagc ttgttcagca cgcatccgcc gatcgagcag cggatccgcc ggttgctcgg
     6241 acgctagctc gagcaccggg ctcaaacgcg agaaagaggg aaacgatgaa agacgagcaa
     6301 ccgactgaga agccaccggc cgcgaaaggc aacggcgagg aacgggacgg cgcgttccgg
     6361 ttcgaagacc ggcggcgttg ggcgcaggga tcggagacgg agccgaacga agcgcagacg
     6421 tcgccgcgaa ctcccacgct gatcgacgaa taccgcgaac gagcggaaag cgccgaacag
     6481 aagctgcagg agtatatcga agcgttcaag gagttcaggc gggagcagga ttcgtttcgc
     6541 gagcggctcg ggcgcgacgt cgatcgtcgg gtcgagctga agttcgggtc tctggttcac
     6601 gacctgctcg agatcctcga caacctcgat cgcgccctcg agcacgtaca agacgttccg
     6661 gaagccgcgg agctggctcg cggggtcgag ctcgcgcgca attcgttcct cgaaacgctc
     6721 gagcgcaacg gcgtcgagcg cgtcgagccg catggtgcgg agttcgatcc gaacgagtcg
     6781 gaggccgtgc ggttggacgc cgtcgacgac ccgcggttgt cgggcaaggt gacggaagtg
     6841 gtccgccccg gttaccgtct gggggagcgc atcgtccggc cggcgcgtgt cgcggtcggc
     6901 cgccacgacg gcccttgagt cgcctcagcg acccagctgg tacagcgcga ccgccgctcc
     6961 ggccccgcac aacgtgttgg cgaagttgag cagtccgtgg tcggccggtc gcgccgagcg
     7021 ccacaggcca ccgacgagcg actcggccat cgcagcgagc caggcgccgg cggccaccgc
     7081 cgccgaggcg gcgagcggcg tcatatcgca gaccaccccc gttgcggcga tcgtagcggc
     7141 ggccgcggcc gcggcgagcg ttccgacgga agataccgcc cccggagttc ccagcgggac
     7201 gggtttcggc ggaaccaggg cccacgccct ccgagcgtaa gcctgaccga tctcggtcgc
     7261 gacggtgtcg gccagtgccg tcgcgagtga ggccgtcagc gcgatccgaa acaccggatc
     7321 ggacggggtg acggcggcca gtatcgcgaa gatcgccgcc gccgaggtgt tggccagcgc
     7381 ctgcgccgcc ccgcgccgcc ccgccgaccc ctcggccagc cccgcaccct ccttgcgcgc
     7441 gcgaccgagc cgcgtggcga tcgcagcgat cacgacgacc agagcgaaga tcgtcaacga
     7501 gccccagccg agggccgcga gcaggatcgc agcgagaccg agaccggcga tcgcaccgcc
     7561 gacggtgacg atccgcagcg agaacgcagc gccggcggcc gccgccagca acgggaaccc
     7621 gagcggcagt cgttcggcgg ccagccgaac gatctccggc caccggccag gatcgacgac
     7681 gctcatcacc gacagaaccg cgcccccgac gagcggcacg gtcaggttgt cgtcgacccc
     7741 gctgtcgaga ctctcgacgc cggccgcggc gatcgccgcg accaggcaac cgccgagcaa
     7801 ccagcgctcg tcagccacgc ccgtcgcgag gaacgacgcg ccgatgcgtc cgccgggatc
     7861 tccgtcgagc gccgcgagct gcgtccaacg caacagcagc gccgaccccg ccgtacccca
     7921 ggcgatgaag gccagcgacc cgacgaccgt tttccgtcga ttccacgcaa gccgcggccc
     7981 accgacgacg acgcccgtga tcgcggccat cccgtcaccg aacgcgacca aaccccagac
     8041 ggcggccgcc agctccagcc gctcgcggaa gacgacgatc agcgcgagga ctgcgcacgg
     8101 gtaacccgcg acgccccagg agaacccgcc cgcgcgctcc cccgcccgca gcagagcgcc
     8161 acgggtcagc ctgtggagcg ccagcagatt gaacagcgcg gcggcgaacg cgccggccgc
     8221 ggcctgccac ggctctaacc atcggagcgc gagcgccagc ccgccgaatg cgacgtggat
     8281 cgacttccgg gtcagctctc ccgcgcgctc atccatcgcg cggagcgagg tatgcggcca
     8341 gcgcttcgtc ccacgaccgc agtcggcgac cgagccgtgc cgcgagctcg gtcgtgtcga
     8401 gcacgctgta tgccgggcgc tgaggtccgt cgtccggcgt cggtcgctcg acgacttcga
     8461 cacgctcggc gaatccgacg atccgcaccg cggcacgcgc cagctcgaga cggctacagg
     8521 cgccgtcatt gaccaggtgc agcacgccgg ttgagcctcg gtcgaccagt tcgacgagcg
     8581 ccgcggcgag gtccgaggtg aaggtcgggg acgagaccgg ttggcgtacc acttcgacac
     8641 gccccccttt tctcgcctgg gccagcaccg cgtcgacgta atgcggtcga ggacgacgtc
     8701 ccgggccgaa cagcgtcgag gtgcgcacga tcagcgattc cgggtgggcg cgcgacaccg
     8761 cccgctcacc ggccagcttg gagcgtccgt agacctgcag cggacggggt tcgtcgccct
     8821 cgacgtaggg ccggttcgcc gtgccgtcga agacgaagtc ggtcgagatg tagacgaagg
     8881 cgatcccgcg gcgccggcag cggagcgcca gatcggcggg tgcgtcgcga ttcagcagga
     8941 atacctgttc gcactgatcg ggcctctccg cgcgcgtcac gtcgctgtag gccgcggcat
     9001 tgatcaccgc cgccggacgg tggcggtcga gccaggcgtc gacccgccgc gggtcggtca
     9061 ggtcgagctg gcggcgggtc ggcgcggacg agcggcgccc gcgcgccgcc agctcgctct
     9121 gcagtgcctc gcccagcagg ccggacccgc cgacgaccag gatgggttcg ggttccacca
     9181 ttccgacgga ctgtatcatc cgtccccccg gacaaacgtg ctagtaggtg acctgcagga
     9241 tcttgccgat gcccttcagg tcgactcgaa agcggaagtc ctcccgttcc gaaccggtgt
     9301 aggtccggtc gaggctctcg agatagaccg tgcagcactg ggtgctgtac tcgagccgcc
     9361 acgcgcgcga cgggaccgtg tcctggccgg gctgcgggtc gaaatcgacc gtcccgtcga
     9421 gcagcaagcg gaacttgccg cgccagaacg acaaaccggt ggtcaggcgg aactgcgtgt
     9481 cgtcggtgtt cggtgcgagt gtcgccgcat cgacgccgag cccgttgcga tggaccaacg
     9541 agaagcggac ctgcgccagc tgtcgtcgaa tcccgccgga cagcgtcgca ccgctgaatt
     9601 gcttccacag aatgtcgtag tcgccacgca agtcgaccgt ggtcgtcgca tccggattgt
     9661 agcgggcggt gatccccacg ctcgaatacg ggctcgtcgc ctcgacgacg ccgtcccggt
     9721 cgaggtccgc tgtgctcagg tcctggtcga acgagcggct ttggccgatc tcgaggtttg
     9781 cgacctcgac cgcctcgttc ggcggaggag gagcgtcact cggctccggg gcgggcggcg
     9841 ccgaggggtc ggtcgagctg acggtcccgt caggcagcac gatcgcctcg ttcgagtcga
     9901 tgacgatcgt ctccgcagct cgcgggcgct tgacgaacag acgctgcgcg atcgagtagc
     9961 gcatcgcgtt acccgcgccg ttgaagacgt cgacctcgtc gaaccggatg atctcgtccg
    10021 ccctggcgaa ggagtcctcg tagccgtagg tcacccgacc ctcgatcgcg tgcttgaact
    10081 ggcggcgatt cggctgcccg tagatgcgcg ccagcttcgg cccgacgagc agcaggtttg
    10141 ccccccacag attgcgaatg aggtcctgat cgagggtcga ccgcacgctc ggcccggccg
    10201 ggttgaacgg gttggatgtc atcgtctgcc gttccgtgta gtaggtgaag cgatagaacg
    10261 cgcgtggcgt gacatcgagc catggggtcg gcgaccaggg caaggacacc gtcgggaaca
    10321 gatcgccgcg caagtactcc gctcgaaacg gtgggcggcc gctgacgtcg tcctcgcgtt
    10381 ggaggatcga agcggccgat gcctcgtact gcagatagac cggcgacttg cccaaccggt
    10441 tgctccgacc gcgaaactcg agctcgggca gcgtcgtctg cagcaacgtc gacccgtcgg
    10501 cgaacaactg ctcgcgtctc aactcgcgcg cgttgatgct ggtccagcta ctgttgcgcg
    10561 agaattcgac gcgctgcaga atctgcggcg tcgagatcag gttcagctcg cgttcgtagt
    10621 cggtgtagta gttgaagtcg ctgaccgcat tgatgtcggc ggtcatgcga aagccgttgg
    10681 tgaactcctg ggtctgctga tagacgaggt tgtagcggtc gccgatcagc tctcccgtcg
    10741 tatcggtcga gacctggtca tcgatgtaga acccctgcag gcgcgccgct ccgcgccggt
    10801 tcgggatcat gcgaaagtcg ccgcccatgc cgaatccggc gtcggtgaag tattcgccga
    10861 acagcgtgac gtccgcgctg cgtccgatcg ggatgaacag gggcagggaa atggtctggc
    10921 ccagtttctc gttggtaccg aactccggca gtagcagtcc caacgatcgg tcctgcttca
    10981 ccggccagat caagtacggc gagtagaaga acggcacgct gctcgcccgc gccacgacgt
    11041 tccacattcg cgcgtagcgc tcgatcgtaa ccgtcgcgct ggtcaccgag aacgaccagt
    11101 agggcacggg ttgggtgcac gtcgtgaccg tcgccgactc gagcttcacc gtccggtcgc
    11161 cgatcttctc ggcccgcttg gcccagaaca ggaaatcgcc ctgaacttgg ccgatcgcct
    11221 tttcgatcat ccctcgttcg gcgtcgagat cgtaggtcaa gcgcgcgccg gagatacggt
    11281 tgccctccca caccaccaga atgttgccgg tggcttcgat gtagcggccg tcagtcagcg
    11341 tgagtcggtc agcctggatc tgctgctcgc gccagatgat cgtgaccttg ccgtcgaagc
    11401 ggtagtcccg ctccgcggtc tgacgccacg gtccccagac ctggatctcg aggtctttga
    11461 gcatctcgtt gcgcggcagg gtctcgggct cgcgctccgg tggcgtagcc cgatcgtcgg
    11521 attgggaggt cgcgagcgat ggaacggtca gcaggaccac ccacagcagc gcccggatcc
    11581 ggatggtcgt gggggatggg aagaggatca tcaggcgacc aatcggtcca gtttcgcgcg
    11641 atggcgggtc agtttagctt cacgctcgcc acggaacaag cgcgccgcgc cgatcagaac
    11701 tggtcctgaa aggggatgtg gttcgccggc gcgccgagca gtggctcggg atcgacggtc
    11761 accggaccgg gtcgcagcat cggcaccgcg cggcgccgct cacgtcggtc gagcgaccag
    11821 gccccgagct ggtccgccag atcgggtggc agccagccgt agacccggcg acagcctcgg
    11881 cggtaggcgt cgtccgcggc ccgcatcagg atcgcggcca tcccgcgcgg atcgccgtcg
    11941 accgaagcga cctcacgcac gttccactcg cctcgtcctt cgacggcgat gacgtagccg
    12001 gcaaaccggc cgcatctcga cgcgatgcgc cactcgtgtc ggatcccggc tccgcggacc
    12061 cggccgaaga aagcgcgcga gcggacccag aggaacttcc agtgggccgc gtcgcgcagg
    12121 atcgcgatcg gcctcgaccg tgtcgatgcg gcgtgggccc gttgaaacag ctcgagctcc
    12181 gatgcgagaa ccggccgcag tccgatttcc tcgaccgcgg gctcgcccgg cgtcagccgg
    12241 cccagatact cgggcgccgg cagggcgcgg aaaccgaggt cggcatagaa ccgcgtaccg
    12301 atgtcggaga agagcagtgc ggcgaggtca ccctgctcgc gcgatcgttc gaccccggcg
    12361 gcgagcatcg ccgaggcgaa tcctcgtcgg cgaaataccg tcggggtgaa caccgccccg
    12421 aagacggagg cccgaccgac ccggtcgccc agacgcagcg ccggacgata gaccttcatg
    12481 ctgctcagtg cgcggccgcg cccgtcgcgc caaacgtagt acgccgcgtg gtcgcggcac
    12541 cacggcgtcg ccccgacctc gtcccacagc tcccggtagt cggcgagaga cagccctccc
    12601 ccccacaaca ggtacgattc gcgagcgatt ccgtcgatct gcgctcggga agccagctcg
    12661 aggcgaggca tccgcacctt cccccgatcg gtcgcgaatg ccgaccggtt cccgtcgagt
    12721 gtagcggggg acgagacgaa acgggagccg cgagccgccg gcgcgatcgg tcaggaagcg
    12781 accttcgggc agcgacaacg ttcgaggcgg cggttgcaga cgaaacaatg gccgtttttt
    12841 tcgaagaact cgaccatctc ctgctgccac gcgtcgagaa tcgacaagcg gtcgagtacc
    12901 gtcaacctgc cggagagcga atcgtcgccc atccaacccc gcatgttcga caacgaattg
    12961 tggtacgcgc gcctgagttc gaagatgttc atcgtgattc catcccgatt cgacgggcgt
    13021 cgatcatcga gaggcaatgt acatcgctcc ctcgggacgg tctagaacga gcacacggcc
    13081 gtgctgcgga tccaccgcgc gccggtcaga cctgtggaaa agctgtgagc ccggcctcgg
    13141 cccgatcggg aaccggcgct tctacccgcc gcccggcgca actcggggta tcaggcaagt
    13201 acttgaacaa caaggagttg ggtgaggtcc cgcggccccg gccccgacgc ttgcgcggct
    13261 cccggccgtc gtgcccggat ttccacaggt gtcgaagccg cgagcggccg acgacgatcg
    13321 cgctagccga ggaactcccg caggtcacgg ctttcctccg ccgcttggat cttcttgaga
    13381 gcggtcgcct cgatttggcg cacccgctcc cgcgacagac ccatcacccg gccgatctcc
    13441 tcgagggtgt acggcatctc gcgcccgatg ccgaaccgga gccgaatgat ctcctcctcg
    13501 cgggaggtca gcaccttgag cgtgccctcg accttttcgc ggagctcgcg gtcgaccgtg
    13561 cgttcgagcg gcgacggggc gttcgggtcg gcgatgaacc gcagcagtcc ggggctatcg
    13621 tcctccgacg acatgtcctc gagcgcctgg ggctcgggca cgacgcccag gatctcctcg
    13681 accttggcga tcggcatcct cagcttgcgt gcgatctcgg gcggggtcgg ccggcgaccg
    13741 agggtctcgg cgagctcctt cgagatgcgg gagatcttct tgatcttctc gttgacgtgg
    13801 accggaattc gaatcacccg cgccttgtcg gcgatcgcgc gctcgatcgc ctgcttgatc
    13861 caccagtagg cgtacgtcga gaacttgttg ccgcgctcgt actcgaactt ctcgaccgcc
    13921 ttcatcaatc cgatattgcc ttcctggatc agatccatga aggaaatgcc gtggttcaag
    13981 tacttcttcg cgatgtgcac gacgagtcgc agattggcca ggatcagcgc gtcgcgggcg
    14041 tgattgacat ggtgcttttg ccgcttggcc tgcgagacga gcgccagggt cctggcttcc
    14101 ttgccgctct cgcgcccgta acgcagcagc ttgcgataga acgtctccat gtcatcgagc
    14161 ggccactccc gaccgcgctt cggcccatcg acatctccct cgagacagta ggctttggcc
    14221 gccgccggga gcttgagcgc aatcttggcc agcgcctccc gcgcgtcttg cagttcgcgc
    14281 gcgagatcga cctcctgatt ctcgtcgatc agcggcgtgg cacccatctc gcggaggtag
    14341 atcggcagga tcgccgcggt cggcttgccc ggctcgaccg cgcgctggtg agcggcgatc
    14401 gtcggctgaa ccgactcgat ctcggccggg ctctgccgga cgatcacctc ggccgtctcc
    14461 tccgactcga tcactcgata atctgccttg cgcgtcatgg gctcggactc catttccgac
    14521 attcggaact cgacgggcgc cggacgacgt gtagctcctc gcaggctgga gtatgaaaaa
    14581 tccgggccaa gtttccgacg atcgtgacgc tttcgcggtt aggggcttcc tacgttgttc
    14641 cgctacgccg ttccgaatca taacaccgaa taccagtgac atttcttctt attcctaaag
    14701 gtggtcggga cacgccgccg ctcgctcacc acactcctac tggtccattt tgaccgattt
    14761 atcgccttcg tcggcgaatc acgccgaaac ggcatctcgg tcccctaccc gcctcatgtg
    14821 ttaccgtccg gatcggggat catcggcgat ggacgatctg aaggtgtcga tcgcacagat
    14881 cgcgcccaaa ctgggcgacg tcgaagcgaa cgtcgaactg catctccaag cgatcgacgc
    14941 cgcgcgcgac gccgcatccg atctggtcgt cttccccgag ctgtcgttga ccggctatca
    15001 cctcggggac caggtcccgg agctcgcgct gggggacgat tcggctccgt tacgggcaat
    15061 cgccgacgca tcccgcgcga tcgacgtcgt gttcggttac gtcgaggacg cccccggtca
    15121 tcgcttcttc aacgccgcgg cgtacctgtc ggcgggagag ccggtccacg tccaccgcaa
    15181 ggtctacttg ccgacctacg gcatgttcca ggaggggcgc gagttcgcgg cgggcgctcg
    15241 tcttcgggcg ttcgacacgc gacacggtcg cctcggcctg ttgatttgcg aagacctgtg
    15301 gcacccgacg agcggatggc tcttgaccca gcagggcgcg gaagtcgtga tcaccgtcgg
    15361 caacgggccg acgcgcggta cacgtccggg ggacggaatc accagcgtcg gcgtctggcg
    15421 cgacttggtc cgaaccaatg cccagttcgg caccagtttc ttcctgttcg tcaatcgcgt
    15481 cggctgcgag gacgggctca ccttcggcgg cggctcgacc gttgccgatc cgttcggtcg
    15541 gatcgtgctc gagctacccc cgctcgaacc cgctctcggg cgggtcgagc tgacgtccga
    15601 cgtggtgcgg cgcgcgcgcg cgacctaccc gctgctgcgg gattccgatc tcgagctggt
    15661 cgaccgcgaa ctgaaacgga ttcgctccgc tcgctacggc atcacgatcg aggatcgacg
    15721 agagccgcat gagtgacggg acgatacccg aggcggtcga cggcgacctc gcgatcgatc
    15781 ccgactggac gacgcggatc ctcgcccgct tcgtcgccag cgaggtcggc cgaacgggca
    15841 agcgctcggt cgtcgtcggt ctgtcgggcg ggatcgactc ggccgtgtcc gcggcgctcg
    15901 ccgcgcgcgc cctgggtccg gcccgcgtgc actgcttgct gatgccgcat cgcgacagca
    15961 atccgcactc cgagcgagac gcgcgacgct tcgccgagca gctcggcagc ccgtcggaga
    16021 cggtcgacat ttcgccgctc gtcgacgggt tcgtcgcggt ctcgggagag gtcggtcgcc
    16081 tgcgactggg caacgtgatg gcacgtgccc gcatgatcgt gctgttcgat cgctcggcag
    16141 cgctcgacgc gctggtgctg ggcacgagca acaagaccga gctgctgttg ggatacggga
    16201 cggtgcacgg ggacatggcc tcggccgtca acccgatcgg cgacctgtac aagacgcaga
    16261 tccgcgcgct cgccgctcat ctcgacgtgc cggaagcgat ccggaacaag cccccgtcgg
    16321 ccgatctatg gcccgaccag tcggacgagg aagacctggg attccgctac gacgaagtcg
    16381 atcgactgct ggcgctactg gtcgacgcgc ggatcgatcg gcgcgaggcg gtgcgtcgcg
    16441 gttttgcgcc gggcttcgtc gacggcgtca tccggcagat cgtgcgctcg caattcaagc
    16501 ggctgccccc ggtgatcgcg aaggtctctt cccgatcgat cggctgggat ttccgctacc
    16561 cgcgggattg gctgagctga cccgctcagt ccccgtcggc tgtcgcggtg gagaggattc
    16621 ccgacgcgcg cgcgatgc
//
LOCUS       DASPGN010000036        10904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_592820, whole genome shotgun sequence.
ACCESSION   DASPGN010000036 DASPGN010000000
VERSION     DASPGN010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10904
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592820"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VD788_01145"
     CDS             <1..614
                     /locus_tag="VD788_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXV74896.1"
                     /translation="LILGPLHAVPLLVALELVAGARLVPEAWSASEARATASLAIPAC
                     LAIPVGASLLGALPAATARRAIALVVLGLVAALATGYRRRGPLRRRVAVAAGTASGLL
                     SGFAGVGGPPIVLLVLSGAEQAARQRARLILYFAATQVAATLVFAWRGWIDGVVLWHL
                     LLVAPGFLLGTHIGARRFEPANERRYRRVGLGLLALAAIPALW"
     gene            complement(631..1377)
                     /locus_tag="VD788_01150"
     CDS             complement(631..1377)
                     /locus_tag="VD788_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXV74897.1"
                     /translation="MHVKVVSYNIHRAIGVDRRFRPRRVSEILRHHDADIVLLQEVDE
                     GVPRSRELDLAREFAVDLDYPHYAVGHNVALRKGRYGNATLSRFPILRERNIDLTIDG
                     RKRRGCQHTTLEIERVAGQPQHLEVFNLHLGLSARERARQIGRLVHSQEYAGLDPRRP
                     SLIGGDFNDWRGRLHPIFTEILDFACATRRRSQGHSFIRTFPSFSPTGGLDRLYYRGP
                     IRLVWARPCRLAASRMASDHLPVIAEFVLR"
     gene            1471..2436
                     /locus_tag="VD788_01155"
     CDS             1471..2436
                     /locus_tag="VD788_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74898.1"
                     /translation="MTSREIPIIVLAGSDPRPAALPERGRDKHALGGFKAVDVSIAGR
                     PLIRAVVDCLAASGWFRPVYVAGPREVYSGKTGPATIVDTNGSFGENVRHAIECVGPQ
                     HPDRPVAFTVCDILPEPGALAALRAEYGRSAPCDLWFPLVRADQPDRLGASAWKPAYR
                     IVEHEGDRASRVLPGHLVIADVAALRLKFLYRLIDLGYRTRNRSIDQRRGEMVRGVLA
                     DMLYQDLLNVARLRVPSLTWSVLRASIPASNLLRRGAITRRQLEETLRRIMVKRRHRR
                     AHPERRVCVPIVDALFLARDIDTEEEAGELGGTLPDEDAGVGPGC"
     gene            2476..4566
                     /gene="fusA"
                     /locus_tag="VD788_01160"
     CDS             2476..4566
                     /gene="fusA"
                     /locus_tag="VD788_01160"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXV74899.1"
                     /translation="MKQVDLSKLRNIGISAHIDSGKTTLTERVLFYTSRIRAIHDVKG
                     KDGVGAKMDSMELERERGITIASAATYCMWNDHQINIIDTPGHVDFTIEVERSLRVLD
                     GAILVLCAVAGVQSQSLTVDRQMRRYRVPRLAFVNKADRSGANPFRVTEQLRDRLQLN
                     AVMMQIPIGLEADHAGVVDLVTMRAVYFEGENGRDVREDEIPARLVDEARNRRQVLLD
                     AASMFDDELLEAALEDRATPEMIHAAVRKGTIALELCPVFVGSAYKNKGVQPLLDGVT
                     RYLPDPTEVSNEAISLESGTEERFKIEVRPEGKLLSLAFKLEEGRFGQLTYLRIYQGT
                     LARGDTIYNSRTGNEERVGRLVRMHADEMEEIDRAAAGDIVALFGIDCASGDTFTSDR
                     WRVAMSSIYVPEPVIHLSIKPIDRKAEGNLSKALTRFAKEDPTFRRRVDEESGETIIS
                     GMGELHLDVYVERMKREYNAAVETGAPQVAYREAISQRADFDYTHKKQTGGSGQYGRV
                     VGYIEPLENGEYEFVNEVRMGAIPTEFIPSVDKGFRSMLRKGGLIGFPITGLRVVIND
                     GAAHSVDSSDNAFQAAARGALRATYLKAKPQILEPIMSVAVEGPGEFQGAFVKTVMQR
                     RGIIVGTTETEGFVRVEADVPLSEMFGYSTVLRSATQGQAEFSMEFSRYAPVPDERAE
                     ELIATYGSSKQGKS"
     gene            4580..5281
                     /locus_tag="VD788_01165"
     CDS             4580..5281
                     /locus_tag="VD788_01165"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HXV74900.1"
                     /translation="MHRKELNERSPLRVLDQSIHGGLGRGNIGVVIARHGVGKTAFLV
                     GVALDDLMRGRNVLHVSLEQNADKVRTYYDEIFAELCHTERLEDVWTVRLSLERHRRI
                     HSYLSGTFTLAKLHDALEFMGRDGQFVPHTLVIDGYDLGSATTADLAGLREIARQFDV
                     EMWMSAVTSRGSGRNQRGIPEPVAHYEDAFDVVLTMAHDGKAVHVGLHKDHDNREVPE
                     LKLALDPTTMLLIRE"
     gene            complement(5235..6173)
                     /locus_tag="VD788_01170"
     CDS             complement(5235..6173)
                     /locus_tag="VD788_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXV74901.1"
                     /translation="MENRGFEYRERVDRRADGRTLLEHLARRYTHSSRERWAERILSG
                     QVLVDGERSPPDRLLGRGQRVVWRRPGWVEPAVPLGFAVLFEDDRLLAVAKPAGLPTL
                     PGGGFLEHTLLALVRRHYPSASPLHRLGRWTSGVVLCARSREAGAALSAAWRDRRVIK
                     RYRALAVGRPARRRFRVDYPIGPVAYPPLGTLFAASPAGKPATSVVEVVEQRQGEFLA
                     DVTIETGRPHQIRIHLAAAGHPLAGDPLYAVGGLPCATGDALPGDPGYRLHAASIEFE
                     HPASGARVTVACRTPVDLCPSVTRGSAASSSGRAPV"
     gene            6291..7532
                     /locus_tag="VD788_01175"
     CDS             6291..7532
                     /locus_tag="VD788_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXV74902.1"
                     /translation="MSQNRIVLVIDYDPRSIQATAEPLRRAGFQVEVRNDGEAGLRAF
                     RELSPALVLIEPMIPRKHGFQVCREIKRSPIGRTTPVVITTAFYRGRRHELAARREYG
                     CDDYLEKPLGAEELLARCRALLERSPVVVSAGGGPREARPLDQLTGMPEPPEMPEPDQ
                     LDRPAFPSLGEITDDEIEARLDGLIDDGEASAVDEPRLDPAAPPAWVAKEKATRRAPV
                     GASRVVVSAALVDAGGASPMARRPRWPLWVAVVAVVCLLTGGAMLWWLADTAPQLGPP
                     LQGSLPATATAEVPPSTGTGLHPSTAREVQPSGGRDGSSLARVHELSGVDRAPVPLRR
                     DPPRYPPPARETARQGTVAMRLLVDETGAVREVELTDGTASDELNHAALDAARGWSYT
                     PASKDGANVAVWIEESVEFKL"
     gene            7620..8615
                     /gene="add"
                     /locus_tag="VD788_01180"
     CDS             7620..8615
                     /gene="add"
                     /locus_tag="VD788_01180"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HXV74903.1"
                     /translation="MTDSLSTLHKVELHQHVDGSIPVRTTWRLMRRHGLAPVARLREM
                     RRLLQLQPDERGSLLSYLDKFHYPMWITQFYENIIDVTYEIIKAAHATGVRLLELRYS
                     PAIHTFAGLSLRQSIRGVLSGMNRAQRELPDLELGLIVISMRQHGPHIAKIIARQALA
                     EAQHLHKRCGVVGFDLAGAERGNPPRLFREAYAIAREGRLGLTVHAGEDEGPRRIWEA
                     IDDLGADRIGHGCSAIEDEALMARLARDRILVECCPTSNYQTGAVEPGRPHPILRFLE
                     RGIPVAVCTDNTTVSATDQNRENGLLLADLGLEQLARIHRDAADYSFIRGYRARR"
     gene            8792..>10904
                     /locus_tag="VD788_01185"
     CDS             8792..>10904
                     /locus_tag="VD788_01185"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXV74904.1"
                     /translation="MKGHVTKVAVLGSGVMGGTIAAHFANAGIPALVLDIVPDAPPPD
                     GGHAAVGPGDRTVRNRLAEAALARLSGMRPAALFTASRRRLLEAGNLEDDLGRLAEAD
                     WVIEVVREEMAIKKKLLAAVVPHLRADALLTSNTSGLSLTEMAESLPTSVRPRFAGTH
                     FFNPPRYMRLLELIPTRFTSSDVVAALAEIASVRLGKGVVLAKDTPNFIANRIGIHSL
                     MATIRVMQESGMTIEQVDALTGKAMARPKTATFRLADLVGVDVLALVADNVRRRTNDE
                     SNELFAVPDFLRRMIERGLLGDKSGGGFYKKVGGPDKKILALDLDTLEYRPTVKPAFP
                     ELAAALAAGRLDQRLQELVRGPGEAARVAWRIVAPTLVYAASRLGEIADDAETIDRAM
                     RLGYNWALGPFEVWDALGFRATAERLERDGYVTPDWVRALVVSGAESLNRVDGNRVST
                     PTFEPGRSAPVDRDPRAVSVELLRRAGGELRRNPSASLLDAGDGVLLLEFHSKMNAID
                     EHTIEMISTAAEEAERNWIALVVANDGESFCAGANLLMLAGLAEAGDWESIERIVRSF
                     QAANDRLERCAVPVVVAPHGIALGGGCEVVLAGHAIRAAAETYLGLVEVGAGLVPAGG
                     GCMRLYRNLLDRTSDRDPYPALETAFRTIGMAKVAGSAEEARELGFLRDGDGSSMNPE
                     HRIAQARVAARALADTGHAPPQ"
BASE COUNT         1717 a         3693 c         3866 g         1628 t
ORIGIN      
        1 gcctgattct gggaccgctc cacgccgtac cgctgctcgt cgcgctcgag ctcgtcgcgg
       61 gggcgcgact ggtgccggag gcgtggagcg cgagcgaggc acgggcgacc gcgtctctgg
      121 cgattccggc gtgtctcgcg atcccggtcg gggcgtcgct gttgggcgcg cttcccgcgg
      181 cgacggcacg ccgcgccatc gcgctcgtcg tgctcggact cgtcgcggcg ctcgcgaccg
      241 gctaccggcg acgaggaccg ttgcgccggc gggtcgccgt cgcggcaggg accgcgagcg
      301 gtctgctctc cggcttcgcc ggcgtcggcg gtccgccgat cgtgctgctg gtgctgtcgg
      361 gggcggagca ggccgcgagg cagcgggcca ggctgatcct ctacttcgcc gcgacgcagg
      421 tcgcggcgac cctggtcttc gcctggcgcg gctggatcga cggcgtcgta ctgtggcacc
      481 tcctgctcgt cgcgcccggc ttcctgctcg gcacccacat cggcgcccgc cggttcgagc
      541 ccgccaacga gagacgctac cgccgcgtcg gtctcgggtt gctcgcgctg gcggcgattc
      601 cggcgttgtg gtgacttccc ggccgcgaca ctaacgcagg acgaactcgg cgatcaccgg
      661 caggtggtcg ctcgccatgc gggatgcggc cagccggcag gggcgggccc acaccagtcg
      721 aatcggaccg cggtagtaga gtcggtcgag gccccccgtc ggtgagaacg aggggaaggt
      781 gcggatgaat gagtggccct gcgagcgccg tcgggtcgca caggcgaaat cgaggatctc
      841 ggtgaagatc ggatgcaggc ggccacgcca atcgttgaag tcgccgccga tcagagacgg
      901 ccgacgcggg tcgagcccgg cgtactcctg cgagtggacg aggcggccga tctgccgcgc
      961 acgctcccgc gccgacagac cgaggtggag gttgaacacc tcgaggtgtt gcggctgccc
     1021 cgcgacgcgc tcgatctcga gcgtcgtgtg ctggcaaccc cggcgcttgc gaccgtcgat
     1081 cgtcagatcg atgttgcgct cgcgcaggat cgggaagcgg ctgagcgtcg cgttgccgta
     1141 gcgacccttg cgcagcgcga cgttgtggcc gacggcgtaa tgcgggtagt ccaggtcgac
     1201 ggcgaactcc cgcgccaggt cgagctcgcg cgaacggggc accccttcgt cgacctcctg
     1261 cagcagaacg atatcggcgt cgtggtggcg gaggatctcg ctcactcgcc gtggtcgaaa
     1321 acgccggtcg acgccgatcg cccggtggat gttgtaggag acgaccttga cgtgcacctg
     1381 agcttccctg tcccgtcgcg gctcgccccg gcctacctcg gatcggacgg gcgattgtga
     1441 cacgattcgt gcgaccatag cgcgagggaa atgacgagcc gagagattcc gatcatcgtc
     1501 ctggccggca gtgatccgcg ccccgcggca ctgccggaac gcggccgcga caagcatgcg
     1561 ctcggcggct tcaaggccgt cgacgtgtcg atcgccggcc ggccgctgat ccgcgcggtc
     1621 gtcgattgcc tggcggcgag cggctggttc cgcccggtct acgtcgccgg acctcgcgag
     1681 gtctattcgg gcaagaccgg gccggcgacg atcgtcgaca ccaacggctc gttcggggag
     1741 aacgtgcgcc acgcgatcga gtgcgtcggg ccgcagcacc ccgatcgacc ggtcgcgttc
     1801 accgtctgcg acatcctgcc cgaacccggc gcactcgccg cgctgcgtgc cgagtacggt
     1861 cggagcgccc cgtgcgacct gtggttcccg ctcgtgcgtg ccgaccagcc cgaccgtctc
     1921 ggcgcctccg cctggaagcc ggcctaccgc atcgtcgaac acgaaggcga cagagcgagc
     1981 cgcgtgctcc ccggccatct cgtcatcgcc gacgtcgccg cgttgcgctt gaagttcctc
     2041 taccgtctga tcgacttggg ctaccgcacg cgcaaccgct cgatcgacca gcgtcgcggc
     2101 gagatggtcc gaggcgtgtt ggccgacatg ctctaccagg acctgctcaa cgtcgcgagg
     2161 ctgcgcgtgc cgagcctgac ttggtcggtg ttgcgcgctt cgattcccgc ctccaacctg
     2221 ctgcgtcggg gtgcgatcac acgccgccag ctcgaggaga cgctgcggcg gatcatggtc
     2281 aagcggcggc atcggcgcgc ccaccccgag cgccgagtct gcgtgccgat cgtcgacgca
     2341 ctgttcctgg cccgggatat cgacaccgag gaagaggccg gcgagctcgg cgggacgctc
     2401 cccgacgagg atgcgggagt cgggccgggc tgctagaatt cgccgctcgt caaccacgaa
     2461 cggcgagaac aaggcatgaa acaagtcgac ctgagcaagc tgcgcaacat cggcatcagc
     2521 gcccacatcg actcgggcaa gacgaccctg accgaacgcg tcctgttcta caccagccgc
     2581 atccgcgcga tccacgacgt caaaggcaag gacggcgtgg gcgccaagat ggactcgatg
     2641 gagctcgaac gcgagcgcgg catcacgatc gcctcggcgg cgacgtactg catgtggaac
     2701 gaccaccaga tcaacatcat cgacacgccg ggccacgtcg acttcacgat cgaggtcgag
     2761 cgatcgctgc gcgtgttgga cggcgcgatt ctcgtgctgt gtgcggtggc cggcgtccag
     2821 tcgcagtcgt tgaccgtcga tcgccagatg cgccggtacc gggtgccgcg tctggccttc
     2881 gtcaacaagg ccgatcgttc cggcgccaat ccgttccgtg tgaccgagca actgcgcgac
     2941 aggctgcagc tcaacgcggt gatgatgcag atcccgatcg gtctcgaggc ggaccacgcc
     3001 ggcgtcgtcg acctggtgac gatgagggcg gtctacttcg aaggggagaa tggtcgcgac
     3061 gtccgcgagg acgagatccc ggcccggctg gtcgacgagg cacggaaccg gcggcaggtc
     3121 ctgctcgacg ccgcgtcgat gttcgatgac gagctgctcg aggcggcgct cgaggatcgc
     3181 gcgacaccgg agatgatcca cgccgccgtg cgcaagggaa cgatcgcgct cgagctgtgc
     3241 ccggtcttcg tcggctcggc ctacaagaac aagggcgtgc agccactgct cgacggcgtc
     3301 acgcgctacc tacccgaccc gaccgaggtc agcaacgagg cgatctcgct cgagagcgga
     3361 accgaggagc ggttcaagat cgaggtccgg ccggagggca agctgctgtc gctggcgttc
     3421 aagctcgaag agggccggtt cggccagttg acctacctgc gcatctacca ggggacgctg
     3481 gcccgcggag ataccattta caatagccgc accggcaacg aggaacgggt cgggcggctg
     3541 gtgcgcatgc acgcggacga gatggaagag atcgaccggg cagccgccgg cgacatcgtc
     3601 gcgctgttcg gcatcgattg cgcctcgggc gacaccttca cctccgaccg ttggcgggtg
     3661 gcgatgagct cgatctacgt gcccgagccg gtgatccatc tgtcgatcaa gccgatcgac
     3721 cgcaaggccg agggcaacct gtccaaggcg ctgacgcggt tcgccaagga agacccgacc
     3781 tttcgtcggc gggtcgacga agagtccggc gagacgatca tctcggggat gggcgagctg
     3841 cacctcgacg tctacgtcga acgcatgaag cgcgagtaca acgcggcggt cgagaccggt
     3901 gcgccgcagg tggcctatcg cgaagcgatc agccagcgcg ccgacttcga ctacacgcac
     3961 aagaagcaga ccggcggatc gggtcagtac ggccgggtcg tcggctacat cgagccgctg
     4021 gagaacggcg agtacgagtt cgtcaacgag gtgcgcatgg gggcgatccc gacggagttc
     4081 atcccgtcgg tcgacaaggg cttccgttcg atgctgagga agggcgggtt gatcggattt
     4141 ccgatcaccg gtttgcgggt cgtgatcaac gacggcgccg cccattccgt cgactcgtcg
     4201 gacaacgcgt tccaggccgc ggcacgcggc gcactgcgcg cgacgtacct caaggccaag
     4261 ccgcagattc tcgagccgat catgtcggtt gccgtcgaag ggccgggaga gttccagggt
     4321 gcgttcgtca agacggtgat gcagcgtcgc gggatcatcg tcggcacgac cgaaaccgag
     4381 gggttcgtcc gtgtcgaagc cgacgtcccg ctgtcggaga tgttcggcta ttcgaccgtc
     4441 ctgcgttctg ccacgcaagg tcaggcggag ttcagcatgg agttctcgcg atacgcgccg
     4501 gtccccgacg agcgcgccga ggaactgatc gcgacctacg gttcgtccaa gcagggtaag
     4561 tcctagcgca gggagcaaca tgcaccggaa ggagctcaac gaacgcagcc cgttgcgggt
     4621 tctcgaccag tcgatccacg gtggtctggg tcggggcaac atcggcgtgg tcatcgccag
     4681 gcacggcgtc ggcaagacgg cgttcctcgt cggcgtggcg ctcgatgacc tgatgcgcgg
     4741 gcggaacgtg ttgcacgtgt cgctcgaaca gaacgcggac aaggtgcgga cgtactacga
     4801 cgagatcttc gccgagctgt gtcacacgga gcggctggag gacgtctgga cggtgcgtct
     4861 gtcgctcgag cgccatcgcc gcatccacag ctacctctcc ggcacgttca cgctggccaa
     4921 gctgcatgat gcgctcgagt tcatgggtcg cgacggccag ttcgttccgc acacgctcgt
     4981 catcgacggt tacgatctcg gcagcgcgac caccgcggac ctcgcggggt tgcgcgagat
     5041 cgcgcgccag ttcgacgtcg agatgtggat gtcggcggtc acctcgcgag ggtccgggcg
     5101 caaccaacgc ggaatcccgg agcccgtcgc gcactacgaa gacgcgttcg acgtggtgct
     5161 gacgatggcc cacgacggca aggcggttca tgtcggcctg cacaaggacc acgacaaccg
     5221 cgaggtcccg gagctcaaac tggcgctcga cccgacgacg atgctgctga tccgcgagtg
     5281 accgaaggac acaggtccac cggggtccgg caggcgacgg tgacccgcgc accgctcgcc
     5341 ggatgctcga actcgatcga cgcggcgtgc agccgataac ccggatcgcc cggcagcgcg
     5401 tctccggtcg cgcagggcaa cccgccgacc gcgtagagcg gatcgcccgc gaggggatga
     5461 ccggccgccg ccaggtggat ccggatctgg tggggtctcc cggtctcgat cgtcacgtcg
     5521 gcgagaaatt cgccctgccg ctgctcgacc acctcgacca cgctggtcgc gggcttcccg
     5581 gccggcgagg cggcgaacaa cgtgccgagc ggcgggtagg cgacgggtcc gatcgggtaa
     5641 tcgacgcgaa accgccggcg cgccggccgg ccgacggcca gcgcccgata gcgcttgatc
     5701 acgcgccggt cgcgccaggc ggcagacagc gcggcgccgg cctcgcgcga gcgcgcgcac
     5761 agcaccacgc ccgaggtcca gcgtccgagc cgatgcagcg gcgacgcgct cgggtaatgc
     5821 cgccggacga gcgcgagcag cgtgtgctcg agaaaaccgc cgccggggag ggtcggcagt
     5881 ccggccggct tggccaccgc cagcaaccga tcgtcctcga acagcacggc gaagccgagc
     5941 gggacggcgg gctcgaccca tcccggccgg cgccaaacga cccgttggcc gcggccgagc
     6001 agccggtcgg gagggcttcg ttcgccgtcg accaggacct gcccgctgag gatccgctcc
     6061 gcccatcgct cgcgcgagct gtgggtgtat cgccgtgcca gatgttcgag cagggtccga
     6121 ccgtcggccc gtcggtcgac ccgttcccgg tactcgaagc ctcggttctc cacggcctct
     6181 ctcctccgac cggccgaagc gtactacgcc ggcggttgaa agggcagacc gtgaagccta
     6241 agttgggcga tgacgtggtg cggccgcggg ttgcgccgcc gggaaaccag atgtcgcaga
     6301 atcgaatcgt cctggtcatc gactacgacc cgcgcagcat ccaggcgacc gcggagccgt
     6361 tgcggcgcgc cggttttcag gtcgaggtgc ggaacgacgg cgaggccggg ctccgcgcgt
     6421 ttcgcgagct gtcgccggcg ctggtgctga tcgagccgat gatcccacgg aagcacggtt
     6481 tccaggtctg tcgtgagatc aagcgaagcc cgatcggtcg caccacaccg gtcgtgatca
     6541 cgaccgcgtt ctaccgcggg aggcggcacg aactcgccgc gcgccgggag tacggctgcg
     6601 acgactacct ggaaaagccg ttgggcgcgg aggagctgtt ggcccgatgc cgcgcgctgt
     6661 tggaacggtc gccggtcgtc gtctcggccg gcggtgggcc gcgtgaggcg cgcccgctcg
     6721 accagctgac cgggatgccg gaaccgccgg agatgccgga gccggatcag ctcgaccggc
     6781 cggcgttccc ctcgctgggc gagatcaccg acgacgagat cgaggcccgg ctcgacgggc
     6841 tgatcgacga cggcgaggcg tcggcggtgg acgaaccgcg gctcgacccc gccgcgccgc
     6901 cggcgtgggt cgcgaaggag aaggcgaccc gccgcgcccc cgtcggcgcc tcccgcgtcg
     6961 tcgtctcggc cgcgttggtc gatgccggtg gcgcctcgcc gatggcgcgc cggccgcgct
     7021 ggccgctgtg ggtcgccgtc gtcgcggtcg tttgcctgct gacgggcggc gcgatgctct
     7081 ggtggctcgc ggacacggca ccgcagctcg gtccgcctct ccaaggctcc ctcccggcga
     7141 ccgccactgc ggaggtcccg ccgtcgacgg ggaccgggct ccacccgtcc acggcgcgcg
     7201 aggtgcagcc gagcggcggc cgggacggat cgtcgctcgc tcgggtgcac gagctgtcgg
     7261 gcgtcgatcg cgccccggtc ccgctgcggc gcgacccgcc gcgctatccg ccaccggcac
     7321 gcgagacggc ccggcagggg accgtggcca tgcgcctgtt ggtcgacgag accggtgccg
     7381 tgcgcgaggt ggaactgacc gacgggacgg cgagcgacga gctgaatcac gccgcgctcg
     7441 acgcggcgcg cggctggagc tacacaccgg catcgaagga cggcgcgaac gtcgcggtgt
     7501 ggatcgaaga gtcggtcgag ttcaagctgt gagccgaccc tccctgcgga acgcgatcac
     7561 aggcagtact gggacggcct tctgagcgac gtccgcctgc cacccgcgga gttcgacgga
     7621 tgaccgattc cctcagcacg ctgcacaagg tcgagctgca tcagcacgtc gacggctcga
     7681 tccccgtgcg tacgacctgg cgcttgatgc gccgccacgg gctcgcaccg gtggcgcggc
     7741 tgcgcgaaat gcggcgcctg ttgcaattgc agccggacga gcggggctcg ctgctctcct
     7801 acctcgacaa gttccactat ccgatgtgga tcacgcagtt ctacgagaac atcatcgacg
     7861 tcacctacga gatcatcaag gcggcccacg ccaccggcgt gcgactgctc gagctgcgtt
     7921 actcaccggc gatccacacc ttcgccggcc tgtcactgcg gcagtccatc cgcggcgtgc
     7981 tgtccgggat gaaccgcgcc cagcgcgagc tgcccgacct cgagctgggc ttgatcgtga
     8041 tctcgatgcg gcagcacgga ccgcacatcg ccaagatcat cgcccgccaa gcgttggccg
     8101 aagcgcagca cctgcacaag cgctgcggcg tggtcggctt cgatctggcg ggggccgagc
     8161 gtggcaaccc accccggctt ttccgcgagg cgtacgccat cgctcgcgag ggacggctgg
     8221 ggttgacggt gcacgcgggg gaggacgagg gaccccggag gatctgggag gcgatcgacg
     8281 acctcggcgc ggaccgcatc ggccatggct gctcggcgat cgaggacgag gcgctgatgg
     8341 cgcggctggc gcgtgaccgc atcctggtcg aatgctgccc gacgtccaac taccagaccg
     8401 gggcggtcga acccggacgg ccgcacccga tcctgcgctt cctcgagcgc ggcatcccgg
     8461 tcgccgtctg tacggacaac accacggtca gcgcgaccga ccagaaccgc gagaacggtc
     8521 tgttgctcgc cgatctcggt ctcgagcagc tcgcgcggat ccaccgcgac gcggccgact
     8581 acagtttcat ccgcggctat cgcgcgcggc ggtgagatgg tcgccgtctt gccgcgttgc
     8641 ggcaaggagt tggtcaatcc gcggaaacac cgtaagtgac tttagtaccg atgtttagtg
     8701 ttctggacgc cgcggtgcgt tattgacacc cacacgatcg aaacctaaca tcgaccgtgg
     8761 ccgccgaccg attgctcgcg gaggaaacgc catgaagggg cacgtcacca aggtcgccgt
     8821 cctgggctcg ggggtgatgg ggggcacgat cgccgcgcat ttcgccaacg cgggtatccc
     8881 cgccctcgtc ctcgacatcg tgccggacgc gccgccgccg gacggcgggc acgccgcggt
     8941 cgggccgggc gatcgcaccg tacggaaccg gctggccgag gcggcgctcg cgcggttgtc
     9001 cgggatgcgc ccggccgcgt tgttcacggc ctcgcgccgg cgactgctcg aggccggaaa
     9061 cctcgaggac gatctgggtc ggctggccga ggccgattgg gtgatcgagg tcgtgcggga
     9121 ggagatggcg atcaagaaga agctgctggc ggccgtcgtc ccgcacctgc gggccgacgc
     9181 gctgctgacc agcaacacct cgggtctgtc gctgaccgag atggccgaat cgctccccac
     9241 gtccgtccga ccgcgtttcg ccggaaccca cttcttcaat ccgccgcgct acatgcgttt
     9301 gctcgagctg atcccgacac gcttcacgtc gtccgacgtg gtcgccgcgc tcgccgagat
     9361 cgcgtcggtg cggctgggca agggcgtggt gctggccaag gacacgccga acttcatcgc
     9421 caaccgaatc ggtatccact cgctgatggc gacgatccgc gtgatgcagg aaagcggtat
     9481 gacgatcgag caggtcgacg cgctcaccgg caaggcgatg gcgcggccga agaccgcgac
     9541 cttccgcctg gcggacctgg tcggcgtcga cgtgctggcg ctggtcgccg acaacgtgcg
     9601 ccggcggacg aacgacgaga gcaacgagct gttcgccgtg ccggacttcc tgcgccggat
     9661 gatcgagcgc gggctgctcg gcgacaagag cggcgggggt ttctacaaga aagtcggagg
     9721 accggacaag aagatcctgg cgctcgacct cgacacgctc gaataccgcc ccaccgtcaa
     9781 gccggcgttc cccgagctcg ccgccgccct cgctgccggt cggctggacc agcggctgca
     9841 ggagctggtt cgtggtccgg gcgaggcggc gcgcgtcgcc tggcggatcg tcgcgcccac
     9901 gctggtctac gccgcgtcgc ggttgggcga gatcgccgac gacgcggaga cgatcgaccg
     9961 cgcgatgcgg ctcggctaca actgggcgct cggtccgttc gaggtatggg acgcgctcgg
    10021 cttccgcgcc acggccgaac ggctcgagcg cgacggctac gtcaccccgg actgggtccg
    10081 ggcgctcgtc gtctccggcg ccgagtcgct caaccgggtc gacggcaacc gtgtttcgac
    10141 tccgaccttc gagccggggc ggtcggcccc ggtcgaccgc gatccgcgcg cggtctcggt
    10201 cgagctgctg cgacgcgccg gaggcgagct gcggcggaac ccgtcggcca gcctgctcga
    10261 cgcgggtgac ggtgtcctgc tgctcgagtt ccactcgaag atgaacgcca tcgacgagca
    10321 cacgatcgag atgatctcga ccgccgcgga ggaagccgag cgaaactgga tcgcactggt
    10381 cgtggccaac gatggcgaga gcttctgtgc cggcgccaac ctgctgatgc tggccggtct
    10441 ggccgaagcc ggggactggg agtcgatcga gcggatcgtg cggagcttcc aggccgcgaa
    10501 cgatcgtctg gagcgctgtg cggtgccggt cgtcgtcgca ccgcacggga tcgcactcgg
    10561 gggagggtgc gaggtcgtgc tcgcgggcca tgcgatccgc gccgccgcgg agacctacct
    10621 cggtttggtc gaggtcggcg ccgggctggt ccctgccggc ggcggatgca tgcggctgta
    10681 ccgcaacctg ctcgatcgga cgagcgaccg cgacccgtac ccggcgctcg aaacggcatt
    10741 ccggacgatc gggatggcca aggtagcggg cagcgccgag gaggcgcggg agctgggctt
    10801 cctccgagac ggtgacggct cgtcgatgaa ccccgagcac cggatcgcgc aggcgcgcgt
    10861 cgcggcgcgc gcgctggccg ataccggcca cgccccgccg caag
//
LOCUS       DASPGN010000037         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1178178, whole genome shotgun sequence.
ACCESSION   DASPGN010000037 DASPGN010000000
VERSION     DASPGN010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178178"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2021
                     /locus_tag="VD788_01190"
     CDS             <1..2021
                     /locus_tag="VD788_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV74905.1"
                     /translation="GVYQVTMDGYLLSANPALIAMLGYASLEELRTVGATDGLYVDPE
                     LRRRLIEQLKQDGKLVEAEYQLRRRDGSVITITENARLNRDERGQPVGFEGTITDITE
                     RKRAEQQFYEEKERAEVTLQSIADAVITTDRSGTVDYVNPIAEKMTGWPSDEACGRNV
                     TEIVKLVKDGGQEVIDDPVSHALASGDPIHLADQTALVSRRGLEVPIQATVAPIRDRA
                     GQTGGAVIVFSDVSRERRMRRLLSYQAAHDALTGLINRREFESRLTSAIESARMNQGV
                     RHAMIYVDLDQFKVVNDTCGHPAGDQLLRQVTGLLQTRVRANDVLARLGGDEFGVLLE
                     HCTPDQALRIADMLRQSIHDLRFQWGTNTMQIGASIGIVEIDHRTETVATLLSAADIA
                     CYSAKDGGRNRVTVYDPASASARHREMRWMARLTHARDEGRLDIMFQPIVRVAKQSKQ
                     RPHYELLLRLHDENGAMVLPDEFIPAAERFNLMPSLDRWVIEKVLRDYVPSTRDGVEE
                     AAFTVAVNLSGTTLSDPSVLEFLVDALEAHEPTPGVLCFEVTETAAITNLGHASYLMR
                     EMASRGCLIALDDFGSGLSSFNYLRTLPVNYLKIDGKFVQNVATDPVDRSMVEAIVQI
                     GRAIGIETVAERVETPAVLEVLKRIGVGYAQGFLLGRPSPMAVFPHRR"
     regulatory      2110..2196
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2278..>2736
                     /locus_tag="VD788_01195"
     CDS             2278..>2736
                     /locus_tag="VD788_01195"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HXV74906.1"
                     /translation="MEAVRILRALVSSILPAAAILLAMTAAVPDAFAGPGDCKRKGRR
                     SIGCTVGANQPPTINGTPPAQATAGQAYSFTPSATDPEGQSLSFSIANKPPWASFSAS
                     TGRLTGTPSATDLGEHADISIQVSDGRLTSTLGPFYIVVNSGNQAPSIAGA"
BASE COUNT          478 a          952 c          924 g          382 t
ORIGIN      
        1 agggcgtcta ccaggtgacg atggacggtt acctgctgtc cgcgaacccg gcgctgatcg
       61 ccatgctcgg ctacgcctcg ctcgaggagc tgcgcacggt cggcgcgacc gacgggctgt
      121 acgtggaccc ggagctgcgc cgccggctga tcgaacagct caagcaggac ggcaagctgg
      181 tcgaggcgga gtaccagctg cggcgccgcg acggctcggt catcacgatc accgagaacg
      241 cgcgcctgaa ccgcgacgag cgcggccagc cggtggggtt cgagggcacg atcaccgaca
      301 tcaccgagcg caagcgtgcc gagcagcagt tctacgaaga gaaggagcgg gccgaagtca
      361 cgctgcagtc catcgccgac gccgtgatca cgaccgaccg ctcgggcacc gtggattacg
      421 tcaacccgat cgccgaaaag atgaccggct ggccctcgga cgaggcctgc gggcggaacg
      481 tcaccgagat cgtgaagctc gtgaaggacg gcggccagga ggtcatcgac gacccggtca
      541 gccatgcgct cgcgagcggc gacccgatcc acctcgcgga ccagacggcg ctcgtgagcc
      601 gccgcggcct cgaggtgccg atccaggcga ccgtggcgcc gatccgcgac cgcgccggcc
      661 agaccggggg cgcggtcatc gtgttcagcg acgtgagccg ggagcgccgc atgaggcggc
      721 tgctgtccta ccaggcggcg cacgacgcgc ttaccggcct gatcaaccgg cgcgagttcg
      781 agagccggct caccagcgcc atcgagtcgg cgcgcatgaa ccagggcgtg cggcacgcga
      841 tgatctacgt ggacctcgac cagttcaagg tcgtcaacga cacctgcggc cacccggccg
      901 gcgaccagct gctgcgccag gtcaccgggc tgctgcagac gcgcgtgcgg gcgaacgacg
      961 tgctcgcgcg cctgggcggc gacgagttcg gcgtgctgct cgagcactgc acgcccgacc
     1021 aggcgcttcg gatcgccgac atgctgcgcc agtcgatcca cgacctgcgc ttccagtggg
     1081 gcacgaacac gatgcagatc ggcgccagca tcggcatcgt cgagatcgac catcgcaccg
     1141 agactgtggc gacgctgctg tcggccgcgg acatcgcctg ctactcggcc aaggacggcg
     1201 gccgcaaccg ggtgacggtc tacgacccgg cgagcgcgtc cgcgcgccac cgcgaaatgc
     1261 gctggatggc gcgcctgaca catgcgcggg acgagggccg gctggacatc atgttccagc
     1321 cgatcgtgcg ggtcgcgaag cagtccaagc agcggccgca ctacgaactc ctgctgcgcc
     1381 tgcacgacga gaacggcgcg atggtgctgc cggacgagtt cattcccgcc gccgagcgct
     1441 tcaacctgat gccctcgctg gaccgctggg tgatcgagaa ggtcctgcgc gactacgtgc
     1501 cctccacgcg cgacggcgtg gaggaggcgg ccttcacggt cgcggtcaac ctgtcgggaa
     1561 cgacgctctc cgacccgagc gtcctcgagt tcctggtcga cgcgctggaa gcccacgagc
     1621 cgacgccggg cgtgctctgc ttcgaggtga ccgagacggc cgcgatcacc aacctcggcc
     1681 acgccagcta cctgatgcgc gagatggcga gccgcggctg cctgatcgcg ctcgacgact
     1741 tcggcagcgg gctctcgtcg ttcaactacc tgcgcacgct gccggtgaac tacctcaaga
     1801 tcgacggaaa gttcgtgcag aacgtcgcga ccgacccggt cgaccgcagc atggtcgagg
     1861 cgatcgtgca gatcggacgg gcgatcggca tcgagaccgt cgccgagcgc gtggagacgc
     1921 cggccgtgct ggaggtcctc aagcgcatcg gcgtcggtta cgcccaggga ttcctgctcg
     1981 gccggccgtc gccgatggcg gtcttcccgc accgccggtg agccaatctc cggaactgtg
     2041 aacgaatcgc cggtttcccg gcgatctccc gcgtgattgc ggtcccgttc ggggccacaa
     2101 tgaagtctca gtgaaacaag gcaaacccgt cgaaaggcgg ggacgcaaag cctccggtct
     2161 gtcggcccaa cggcccagga tagcggggtt gccacttgaa cacgcgatca cgcgcgtcct
     2221 gcggcgcccc gtcgttcaaa gccaggtcga catcccggca cgacacgggt ggtcgacatg
     2281 gaagctgtcc gaattcttcg agccctggtc tccagcatat tgccggctgc ggcgatcctc
     2341 ctggcaatga ccgcggcagt gccagacgct ttcgccggtc ctggcgactg caaacgcaag
     2401 gggcggcgca gcatcggctg caccgttggc gccaaccagc cgccgacgat caacggcacc
     2461 ccaccggcgc aggcgacggc cggccaggcc tattccttca cgccgtcggc caccgacccg
     2521 gaaggccagt cgctgagctt cagcatcgcc aacaagccgc cgtgggcctc gttcagcgcg
     2581 tccacggggc gactgacggg cacgccctca gccaccgacc tgggcgagca cgccgacata
     2641 tcgattcagg tcagcgatgg ccggctgacc tcgacgcttg ggccgttcta catcgtcgtg
     2701 aattcaggca accaggcacc gagcatcgcg ggcgcg
//
LOCUS       DASPGN010000038         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_593402, whole genome shotgun sequence.
ACCESSION   DASPGN010000038 DASPGN010000000
VERSION     DASPGN010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593402"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VD788_01200"
     CDS             <1..849
                     /locus_tag="VD788_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74907.1"
                     /translation="VILVLGVTAVTRATAPPAQIAVSPSKLELVLRDRPATETLTLMN
                     LGDEQVEIDVAVANWDLDQDNRVRLLEPDEQSLDQWMVINPLHFVLPPGKSQAVRFSI
                     RPRVAPVPGEHRAMIYFNQVLPATNESVVRVRFSVGVAIYAHAGEPTRDGTLNDVMVV
                     SGSNPPVARLDVSSVGSAHVRVAGQYAIYPASSYPGTVLTSWSPETGPQEIETPAAAI
                     VAGALPSKPVLPRTRRQLVLQTFERLAPGEYVLDLNGELHGRPIDLAVPFTIGDPRLV
                     AGSGPD"
     gene            877..>2980
                     /locus_tag="VD788_01205"
     CDS             877..>2980
                     /locus_tag="VD788_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74908.1"
                     /translation="MRNANRVGRRVPVRGIVSLALSLVAVAVYGGETPDERLDTERLL
                     VGAVVGQVEVGPLDVWRVGDDYLIPVEEFADYAGCRLQRDDYGITLRTPLGDARLDAD
                     ELREHEGALYVSARVLEQQLATSVSFDAARYALRFDLPWRPSDGERRVLPIEHEADVS
                     APSASLSTIYADVRHTRFGSREFTQGGTTLGGALGGGYWRVRYQSDFDDQHTLRDYAW
                     IYRGDDHLVLAGNQRVRIHPLLRGIEFTGVQYARTNQDLDLFARRSTPSELLSRQMQP
                     IATFEGYGPAAGFAELWIDGVFVDREPIGLDRRYEFLDVSIGARQSSHVEIRLFDRHN
                     PTVPVEIVEEHRTASAFLLPGGASMHMTGGGIAGNAVQNQLDPLVDDERFAGFYQTRY
                     GLSDRLTLEGALQGGDDGAQLFGGYTLRLNRTFVLGLGVGSSTENAHGYSVDIEGLPL
                     PFRVLGRLQESEAGFDPLYPFYQRDHYLEVGYSGVRNLDVAVIGRSRLNSTVDVDYLL
                     PAASWRPTDAVWLNARPDLDGDYRYDLLYRFRPLTRFSLSSVKDRRLAQLWHGLRPNL
                     QLSANADVGGRYPERYAVLAHGFGRSRWRPSWAAGPLWTGGRPGYLLDGRLTLSPGLV
                     AQARLESSAIGTDEAATSEPRLLVNLFADLGLSGGRLVAASTTAVREGRGGIAGVVHV
                     DAPRGFPRFDLGDLPVSLD"
BASE COUNT          430 a         1064 c         1019 g          467 t
ORIGIN      
        1 gtcatcctgg ttctcggagt caccgccgtt acccgcgcca cggcaccgcc ggcgcagatc
       61 gcggtcagcc cgtcgaagct cgagctggtg ctgcgcgacc gcccggcgac cgagacgctc
      121 accttgatga acctcggcga cgaacaggtc gagatcgacg tcgccgttgc caactgggat
      181 ctggaccagg acaaccgggt ccgcctgctc gaacccgacg agcagtcgct cgatcagtgg
      241 atggtgatca acccgctgca cttcgtcctg ccgccgggca agtcccaggc cgtgcggttc
      301 tcgatccgcc cgcgggtggc gcccgtcccg ggcgagcacc gcgcgatgat ctatttcaac
      361 caggtgctgc ccgccacgaa cgagtcggtc gtgcgcgtcc gcttcagcgt cggcgtcgcg
      421 atctacgcgc atgccggcga accgacgcgc gacgggacgt tgaacgacgt gatggtggtg
      481 tcgggcagca atccgccagt ggcgcggctc gacgtgtcga gcgtcggctc ggcccacgtc
      541 cgcgtggccg gccagtacgc gatctacccg gcgtccagct atcccggtac cgtgctgacg
      601 tcgtggtccc ccgagaccgg gccgcaggag atcgagaccc ccgcggcggc gatcgtcgcc
      661 ggcgcgctac cgtccaaacc ggtgctgccc cgaacgcgcc gtcagctggt cttgcaaacc
      721 ttcgagcgtc tggcgcccgg cgagtacgtg ctcgacctca acggcgagct gcacggccgg
      781 ccgatcgatc tcgccgttcc gttcacgatc ggggacccga gactggtggc cggcagcggc
      841 ccggactgat cgcagtctgc ggttcggaag ctgtcgatga gaaacgcgaa cagggtcggc
      901 cggcgggttc cggtccgcgg aatcgtctcg ctggcgctgt cgctggtcgc ggtcgccgtg
      961 tacggtggcg aaaccccgga cgaacggctc gataccgagc ggctgctggt cggggccgtc
     1021 gtcggccagg tcgaggtcgg tccgctcgac gtgtggcgcg tcggcgacga ctacctgatc
     1081 ccggtcgagg agttcgccga ctacgccggc tgccgactgc aacgcgacga ctacggcatc
     1141 acgctccgga cgccgctcgg cgatgcgcga ctcgacgccg acgagctgcg cgagcacgag
     1201 ggcgcgctct acgtatccgc ccgcgtgctc gagcagcagc tcgcgacctc ggtctcgttc
     1261 gatgccgcgc gctacgcgct ccgcttcgat ctgccttggc ggccgtcgga tggtgaacgc
     1321 cgcgtcctac cgatcgaaca cgaggccgac gtgtcggcgc cgtcggccag cctgtcgacg
     1381 atctatgccg acgtgcgaca cacgcggttc ggctcgcggg agttcaccca gggcggcacg
     1441 acgctcggcg gcgcgctcgg cgggggctat tggcgcgtac gctatcaatc ggacttcgac
     1501 gaccagcaca cgctgcgcga ctacgcgtgg atctaccgtg gagacgatca cctcgtgctg
     1561 gcgggcaacc agcgcgtccg catccatccg ttgctgcgcg gcatcgagtt caccggcgtg
     1621 cagtacgcgc gcaccaacca ggatctcgac ctgttcgcgc gacggtcgac acccagtgag
     1681 ctgttgtcac ggcagatgca gccgatcgcc accttcgagg ggtacgggcc cgcggccggc
     1741 ttcgccgagc tgtggatcga cggggtgttc gtcgaccgcg agccgatcgg gctcgaccga
     1801 cgatacgagt tcctcgacgt gtcgatcggc gcgcggcagt cgagccacgt cgagatccgg
     1861 ctgttcgacc gccacaaccc gaccgttccg gtcgagatcg tcgaggagca caggacggcg
     1921 tcggccttcc tgctgccggg gggcgcgtcg atgcacatga ccggtggcgg tatcgcgggc
     1981 aacgccgtcc agaaccagct cgacccgctc gtggacgacg agcgtttcgc cggcttttat
     2041 cagacccgct acggtctctc cgacaggctg acgctcgaag gcgcgctcca aggcggcgac
     2101 gacggggcac agctgttcgg cggctacacc ctccggctca atcgcacctt cgtcctgggg
     2161 ctcggcgtcg gcagctcgac cgagaacgcc cacggctaca gcgtcgacat cgaagggctc
     2221 ccgctgccgt tccgcgtact cggtcgactg caggagtcgg aggccggctt cgacccgctc
     2281 tacccgttct accagcgcga tcactatctc gaggtcggct actcgggcgt gcgcaacctc
     2341 gacgtcgccg tcatcggtcg cagccggttg aactcgaccg tcgacgtcga ctacctgctg
     2401 ccggcggcct cgtggcgccc gaccgacgcg gtgtggctca acgcgcgacc cgatctcgac
     2461 ggagactatc gctacgatct gctctaccgc ttccgcccgc tgacgcggtt cagcctcagc
     2521 tcggtcaagg atcgacggct ggcgcagctc tggcacgggc tccggccgaa cctgcagctg
     2581 agtgccaacg ccgacgtcgg gggccgctac ccggagcgct acgccgtcct ggcgcacggt
     2641 ttcggccgct cgcgctggcg cccgagctgg gccgccggac cgctgtggac cggcgggcga
     2701 ccgggctacc tgctggacgg ccggctgacg ctcagccccg ggctcgtcgc gcaggcgcgt
     2761 ctcgagagca gcgcgatcgg tacggacgag gccgccacga gcgagccgcg gctgctggtc
     2821 aatctgttcg ccgacctcgg actgagcggc ggccgcctcg tcgcggcgag cacgaccgcc
     2881 gtgcgcgagg gccgcggagg catcgccgga gtcgtgcacg tcgacgcccc gcgcggcttc
     2941 ccccggttcg atctggggga cctaccggtc tcgctcgacg
//
LOCUS       DASPGN010000039         4115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1530200, whole genome shotgun sequence.
ACCESSION   DASPGN010000039 DASPGN010000000
VERSION     DASPGN010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530200"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VD788_01210"
     CDS             complement(<1..459)
                     /locus_tag="VD788_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HXV74909.1"
                     /translation="MPADIFATKGIEYLLVIVFLAALVVFWRLLARMPAPALARAGRS
                     VESAAGRWFELRSERLYHQGHTWVDRERPDVVRVGVDDFARRLIGAPQRVDLPRPGTR
                     LRPGERGFRLIAGGKAIDVLSPVAGRVVERNEEVRRTPALVDDDPYDRGWL"
     gene            complement(461..1714)
                     /gene="hybB"
                     /locus_tag="VD788_01215"
     CDS             complement(461..1714)
                     /gene="hybB"
                     /locus_tag="VD788_01215"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HXV74910.1"
                     /translation="MHTQRKPSDVGRFLLAELRPKGRLLTPFNLVSIPVIALGAVLLV
                     IRFARGLGSVTNLNQEYPWGLWIGFDVITGVAFAAGAYVVTFIVYILNMKKYHAIVRA
                     TILNGLLGYLFYAGALLLDLGRPWNVINPIIGNSFGVSSVLFLVAWHFLLYMIAEAIE
                     FSPAVAEWLGWRRLRKLMASLTLGAVILGITLSTLHQAGLGALYLMAKGKLHPLWYSE
                     FIPLLFFVSSIFAGLSMVIFEGSISQRVFGHRLSSDHRASHDGILLGLARICAGAMFV
                     YLFLQTLVFVHGQRWEYLATGWGAWYLLELVGFVLVPLVLFARASLLGRPRAVRLAAA
                     LTLAGIVLNRLNVTIIGFKWYEAARYVPSWMEVAVTLAVVLAEIWAFRWIVNRMPVLG
                     ESPAWATDEDRAEPSAPPLRLVKEA"
     gene            complement(1716..2582)
                     /locus_tag="VD788_01220"
     CDS             complement(1716..2582)
                     /locus_tag="VD788_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXV74911.1"
                     /translation="MNRRSFLKTVGVSGAMAMTGSAATGGATDHADAERESLAVLVDT
                     TRCAGCRNCELACAEANGLPVPDTGDDSLLERERKPGPGQWSVINRFETDAGEVFVKR
                     QCMHCLQPACASACLTKAMLKTEQGPVVWREEKCMGCRYCMMSCPFDMPTFEYDSAVP
                     RIEKCRMCFDRLARGARPACVENCPAEALTFGPRHELLVEARRRFYAEPGRYVDRIYG
                     EHEVGGTGWLYLSAVPFEQIGFRQDLGDVAYPELTKGFLYGVPVVLTLWPAFLLALSQ
                     ATRDRCHTPTEG"
     gene            complement(2699..3379)
                     /locus_tag="VD788_01225"
     CDS             complement(2699..3379)
                     /locus_tag="VD788_01225"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74912.1"
                     /translation="MTAGVVAYKICDRGFDCEHCPLDIGLRGADWPLVPRRGRIRPET
                     TAFRFPDDRLYLPAHTWARPVDEDRVRCGIDAFGAGLLRHASALVLPAAGATVEQGRV
                     GFWLSAHGRPVPFLSPVSGRVERRNRLAQKRPILVARASYDEGWLLEVHCRDWSEQRS
                     RLVDSARIKQRTRRDMQLLGDQLSRSLAPPGSSLGRTMADGGQPLFDFLDAIDPERFR
                     KLIMPLLV"
     gene            complement(3471..>4115)
                     /locus_tag="VD788_01230"
     CDS             complement(3471..>4115)
                     /locus_tag="VD788_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74913.1"
                     /translation="DYIVHRRAAGREHEPLAADAAVVPVSIESRLLAVPGDVYLSRGH
                     SWVRPRRDRTVRVGAGRLPLHALGPPDALQLPPVGTRVHAGAALVTLSRGARRLTLRS
                     PVDGRIESINREALARPQRVGRRPYDRGWLCTVRPTDLSRALRELLVAEEATDWLRRE
                     LGRLRDLVSGLAQPAAAAVPLRLDGGVPVEGLAGLLDEAQWGEVQSFFDRPSGD"
BASE COUNT          724 a         1438 c         1390 g          563 t
ORIGIN      
        1 cagccagcct cggtcgtacg ggtcgtcgtc gacgagcgcc ggcgtgcggc ggacttcctc
       61 gtttcgctcg accacgcggc ccgcgaccgg cgacagcacg tcgatcgcct tgccgccggc
      121 gatcagacga aagccgcgct cgcccggccg cagccgcgta cccggccgcg gcaggtcgac
      181 ccgctgcgga gcgccgatca gcctccgcgc gaagtcgtcg acgccgacgc gaaccacgtc
      241 gggccgctcg cggtcgaccc aggtgtgacc ctggtggtag aggcgttcgg aacgcagctc
      301 gaaccaacgg cccgccgcgg actcgaccga ccggccggcg cgggccagcg ccggcgccgg
      361 catgcgcgcc agcaaacgcc agaacacgac gagcgcggcg agaaacacga tgacgagtag
      421 gtactcgatg cctttggtgg cgaagatgtc ggcgggcatt tcacgcctcc ttgaccagtc
      481 tgagcggcgg tgcgctcggc tccgctcgat cctcgtcggt ggcccacgcg ggagactcgc
      541 cgagcaccgg cattcgattg acgatccaac gaaaggccca gatctcggcc aagaccaccg
      601 ccagtgtcac ggcgacctcc atccagctcg gcacgtagcg cgcggcttcg taccacttga
      661 atccgatgat cgtcacgttc agccggttga gcacgatgcc ggccagcgtc agcgccgcgg
      721 cgagtctgac cgcgcgcggt cggccgagga gcgaagcgcg ggcgaacagc acgagtggaa
      781 ccagcacgaa gccgaccagc tcgagcaggt accacgcgcc ccaacccgtc gccaggtatt
      841 cccagcgctg cccgtgcacg aagaccagcg tctggaggaa caggtagacg aacatcgctc
      901 ccgcgcagat gcgtgccagc ccgagcagga tcccgtcgtg cgacgcgcgg tggtcactcg
      961 acaaccggtg gccgaagaca cgctggctga tcgatccctc gaagatcacc atcgacagcc
     1021 cggcgaagat gctcgagacg aagaacagca gcgggatgaa ctccgagtac cacagcgggt
     1081 gcagcttgcc cttggccatc aggtacagcg cgcccagccc ggcctggtgc agcgtcgaca
     1141 gcgtgatacc gaggatcacg gcgcccagcg tcagcgatgc catcagcttg cgcaggcggc
     1201 gccagccgag ccactcggcg acggcgggag agaactcgat cgcctcggcg atcatgtaca
     1261 acaggaaatg ccacgccacc aggaacagca ccgagctgac tccgaaggaa ttgccgatga
     1321 tcggattgat cacgttccat gggcggccca ggtcgagcag cagcgcgccg gcgtagaaca
     1381 ggtatcccag caacccgttg agtatcgttg cccgcacgat cgcgtggtac ttcttcatgt
     1441 tcaggatgta gacgatgaag gtcacgacgt aagccccggc ggcgaacgcc acaccggtga
     1501 tcacgtcgaa gccgatccaa agtccccacg ggtactcttg attcaggttc gtcaccgagc
     1561 cgagcccgcg cgcgaagcga atcacgagca acacggcgcc gagcgcgatc acggggatcg
     1621 acaccaggtt gaacggggtc agcagtcggc ccttgggcct caactcggcg agcaagaacc
     1681 gcccgacgtc gctcggtttt cgttgcgtgt gcatctcaac cctcggttgg cgtgtggcag
     1741 cggtccctcg tcgcctgact caacgccagc aggaatgccg gccagagcgt cagcacgacg
     1801 ggcacgccgt agaggaatcc cttggtcagc tcggggtagg cgacgtcgcc gaggtcttgg
     1861 cggaagccga tctgctcgaa cggtacggcc gacaggtaga gccaacccgt gccgccgacc
     1921 tcgtgctcgc cgtagatccg atcgacgtag cgccccggct ccgcgtagaa ccggcgccgg
     1981 gcctcgacca acagctcgtg gcgcggaccg aacgtcagcg cctcggccgg gcagttttcg
     2041 acgcaggccg gtcgcgcgcc gcgcgccagt cggtcgaagc acatccgaca tttctcgatc
     2101 cgcggcacgg cgctgtcgta ctcgaacgtc ggcatgtcga acgggcacga catcatgcaa
     2161 tagcggcagc ccatgcactt ctcttcgcgc cagacgaccg ggccctgttc ggtcttcagc
     2221 atcgccttgg tcagacaggc cgacgcgcac gccggctgca ggcagtgcat gcactgccgc
     2281 ttgacgaaca cttcgccagc gtcggtttcg aagcggttga tcaccgacca ttgaccgggg
     2341 ccgggcttgc gctcccgttc gagcagcgag tcgtcgccgg tgtccggaac cggaagtccg
     2401 ttggcctccg cgcaggccag ctcgcagttg cggcagccgg cgcagcgcgt cgtgtcgacg
     2461 aggacggcca gcgactcgcg ctcggcgtcg gcgtgatcgg tcgcgccgcc cgtcgcggcg
     2521 gaaccggtca tcgccatcgc gccggatacg ccgactgtct tgaggaagct gcggcggttc
     2581 atcacacggt cctcggcggc tcgaagtgcg agtgtttcgg ccgcgagtcg atcgtcagga
     2641 acaacccgac ggttagcagc acgaacagcg tgaccatgat tcgttccttc cgccgcgctc
     2701 acacgagcag cggcatgatc aacttgcgaa agcgctccgg gtcgatcgcg tcgagaaagt
     2761 cgaacagcgg ttgcccgccg tcggccatcg tccgaccgag cgacgatccg ggcggggcaa
     2821 gcgagcgcga tagctgatcg ccgagcagct gcatgtcgcg ccgggtccgt tgcttgattc
     2881 gcgcggaatc gaccagccgg gagcgttgct cggaccaatc gcggcagtgg acctcgagca
     2941 gccagccctc gtcgtacgac gctcgggcga cgaggatcgg tcgcttctgg gccagccggt
     3001 tgcggcgttc gacacgaccc gacaccggcg acaggaacgg gacggggcgg ccgtgcgcgc
     3061 tgagccagaa gccgacgcgc ccctgttcca cggtcgctcc cgccgccggc agcaccagcg
     3121 ccgacgcgtg gcgcaacagt cccgcgccga acgcgtcgat cccgcaccgg acacggtctt
     3181 cgtcgacagg ccgggcccag gtgtgcgccg ggagatacag tcggtcgtcg gggaagcgga
     3241 acgccgtcgt ctccggtctg atccggccgc gacgggggac gagcggccag tcggcgccgc
     3301 gcaagccgat gtcgagcggg caatgctcgc agtcgaagcc gcggtcgcag atcttgtacg
     3361 cgacgacccc ggcggtcatc catacgcacg gctcggcgga gtccggcggg cgttccgatc
     3421 ggcgatcatc agctgctcgg taacccatcg catcctcgaa ccgcgacggt tcagtcgcct
     3481 gacggacgat cgaagaaaga ctggacctcg ccccactgag cctcgtcgag cagaccggcg
     3541 agtccctcga ccgggacacc accgtcgagt cggagcggca cggccgcggc cgcgggttgg
     3601 gccagaccgg aaaccaggtc gcgcagccga cccagctcgc gccgcagcca gtccgtcgcc
     3661 tcctcggcga cgagcagctc gcgcagggcg cgcgagagat cggtcggtcg gacggtgcac
     3721 agccatccgc ggtcgtacgg ccgccgcccc acccgttgcg gccgagccag cgcttcgcga
     3781 ttgatcgact cgatccgtcc gtcgaccggc gagcgcagcg tcaaccggcg agcgccgcgc
     3841 gagagcgtga ccagcgccgc gccggcgtgc acgcgcgtgc cgaccggcgg gagctgcagc
     3901 gcgtccggcg gaccgagcgc gtgcagcggc agacgacccg cgccgacccg caccgtgcgg
     3961 tcgcgacggg gtctgaccca gctgtgaccg cgcgacaggt agacgtcgcc ggggacggcc
     4021 agcagccggg attcgatcga aacgggcacg accgcggcat ccgcggcgag cggctcgtgc
     4081 tcgcgcccgg ccgcccgtcg atggacgatg tagtc
//
LOCUS       DASPGN010000040         5374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_593591, whole genome shotgun sequence.
ACCESSION   DASPGN010000040 DASPGN010000000
VERSION     DASPGN010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593591"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VD788_01235"
     CDS             complement(<1..895)
                     /locus_tag="VD788_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HXV74914.1"
                     /translation="MFDNLSERLQGIFKKLSGQARIGEAVLGETLREVRLALLEADVH
                     VTVVKQLLAAVRERALGDEVLRSLGPGQQVIKIVRDELETLLGGAGLAKLEFSAKPPS
                     VFLMVGLQGSGKTTTSAKLGAWLKKGGHYPYLVPVDVYRPAAIEQLVRVGTQAGLKVH
                     GHDGSGQPLDIAREGIAHARRSGFDTVIVDTAGRLHVDEALMAEIRGLAHGLLPSEIL
                     FVADAMTGQDAVRSAGDFNAALDLTGIVLTKLDGDARGGAALSIRHVTGVPIKFVGTG
                     ERVDEFEPFHADRMVGRILGMG"
     gene            complement(989..2209)
                     /locus_tag="VD788_01240"
     CDS             complement(989..2209)
                     /locus_tag="VD788_01240"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF006803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HXV74915.1"
                     /translation="MSETGFNRIEEVIADLRNGRMIIVIDDEDRENEGDLVCAAEFVT
                     PETINFMATHGRGLICMPMTQDRLDSLEIPLMVQDKTNTARRGTAFCVSIEARHDITT
                     GISAADRATTIRKAVDPATKPSDLSRPGHVFPLLARPGGVLKRAGHTEASVDLCRLGG
                     LRPAAVVCEVMNPDGTMARLPELRIFARRHGLKLITIAEIISYRMRTERLVKRVASPD
                     LPTALGDWRIHAFHYELENQTHVALVMGQPKPDQPVLVRVHSECLTGDVFGSTRCDCG
                     PQLHAAMDRIAAEGCGAILYLRQEGRGIGLANKLRAYELQDRHHKDTVEANLLLGFEA
                     DHRDYGVGAQILYDLGIRRLRLMTNNMGKYVALKGYGLELVERVSLELPPHERNRDYL
                     RTKKQKMGHLLESV"
     gene            complement(2215..2817)
                     /locus_tag="VD788_01245"
     CDS             complement(2215..2817)
                     /locus_tag="VD788_01245"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXV74916.1"
                     /translation="MFSGLIEAVGRVTDVAPRHGATRIGIESSLPAAELVDGESIAVD
                     GVCLTVAERRTDRFLADVVAETLARTTLGRLRPGDPVNLERSLRLGDRVGGHLVLGHV
                     DGTVRVRAVRRRGDDYRMRIDLPAALAPYVPYKGSVALQGVSLTVAGVGRGGFEVALV
                     PHTLEWTTLGRFRAGETMNFEVDLLARYLERLIAQRRTDG"
     gene            complement(2819..3961)
                     /gene="ribD"
                     /locus_tag="VD788_01250"
     CDS             complement(2819..3961)
                     /gene="ribD"
                     /locus_tag="VD788_01250"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HXV74917.1"
                     /translation="MSAGRDWTGRERDWMAQALALAALADGQTSPNPLVGCVLVKQDR
                     VVGRGFHRAPGEPHAEAIAVREAGGEARGATLVVNLEPCAHVGRTLPCSEALIASGVA
                     RVLASMQDPNPLVNGRGFEQLRRAGIEVRVGLLEDEARRLNEPFIRWHERGLPMVTLK
                     AAISADGMLAARDGCSRWISGPEARRFAHRLRWRHDAVLVGVGTVRRDDPRLTVRLGG
                     REAARRRAVFSRSLDLDPRAAVFDESTSSAGRTLVFTPAGADGSRAARLASAEVVELS
                     AGDDAGFVRRALEQLAARGIQSVLVEGGGQTLASFVASGLAQRVALFTSARMIGARGA
                     RPLLDGDAAAAPDEAWRVAREQIVPLGDDLLLLGRLVGPARPGRER"
     gene            complement(3958..4872)
                     /gene="ftsY"
                     /locus_tag="VD788_01255"
     CDS             complement(3958..4872)
                     /gene="ftsY"
                     /locus_tag="VD788_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HXV74918.1"
                     /translation="MALTVLGKLRNGLARTRATIASTLQSVVGSREVDEEAIDELEAS
                     MLAADLGPELTAQVLDEIRARAAAGSLDGAGLTGAVRDVLRGALPDRRPAAAGTTPHV
                     IFVVGVNGGGKTTTVGKLAARERRAGRRVIVAAADTFRAAAIDQLERWVERADAELIK
                     HREKADPAAVVFDALAAARGRGVETVIVDPAGRLHTKTNLMAELGKLARVAAREIPGA
                     PHEVLLVIDATTGQNGLAQAEQFTRAAELSGVVLTKLDGTAKGGVALAIHRRLGVPIR
                     YVGVGEAVDDLLEFDPDAFVDGLLGGER"
     gene            complement(4877..>5374)
                     /locus_tag="VD788_01260"
     CDS             complement(4877..>5374)
                     /locus_tag="VD788_01260"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV74919.1"
                     /translation="EAFETALEHDPFLTDARNFLGAVYTEMGRIDDAEYQFREALEDP
                     AYPTPEKVYLNLALAYVAQGRDEEAISELRTAVELNPKFYKAHYELASLLDRVGRIDE
                     AAREYEVARPDYRNVGEFHYRLGFVYFRLGDRARARESLERAIEVAPGSNSAAQADDI
                     LKMMN"
BASE COUNT          792 a         1961 c         1825 g          796 t
ORIGIN      
        1 cccccatgcc gaggatgcgc ccgaccatcc ggtcggcgtg aaacggctcg aactcgtcga
       61 cgcgctcgcc ggtgccgacg aacttgatcg ggacgccggt cacgtggcgg atcgacaacg
      121 cggcaccgcc gcgcgcgtcg ccgtcgagct tcgtcagcac gatcccggtc aggtcgagcg
      181 cggcgttgaa gtcgccggcg gaccgcacgg catcctgacc ggtcatcgcg tcggcgacga
      241 acaggatctc ggacgggagc aggccgtggg ccagcccgcg tatctccgcc atcagcgcct
      301 cgtcgacgtg caatctcccg gcggtatcga cgatcacggt gtcgaaaccc gagcggcgag
      361 cgtgggcgat cccctcgcgc gcgatgtcga gcggctggcc gctaccgtca tgcccgtgga
      421 ccttcagacc ggcttgcgtg ccgacccgca ccagctgctc gatcgccgcg gggcggtaga
      481 cgtcgacggg aacgagatac ggatagtgcc cgcctttctt cagccacgcc ccgagcttgg
      541 cgctcgtcgt cgtcttgccc gagccctgca ggccgaccat caggaacacg gacgggggct
      601 tggcggaaaa ctcgagtttg gccaggccgg cgccaccgag cagcgtctcg agctcgtcgc
      661 ggacgatctt gatcacctgc tggcccggac cgaggctccg cagcacctcg tcgccgagcg
      721 ccctttcgcg gaccgccgca agcagctgct tgacgaccgt gacgtggacg tcggcctcga
      781 gcagcgcgag gcgcacctcg cgcagcgtct cgcccagcac ggcttcgccg atccgtgcct
      841 gtccgctgag cttcttgaag atgccctgca gccgttctga caagttgtcg aacaaggcag
      901 ccccgtcgcg agccgaaccg cgcgatcggc ggattatatc ggccggccag aggaccgtcc
      961 agcgagcggc cgggcgcgga acgccccgtc agaccgactc gagcaggtgt cccatcttct
     1021 gcttcttggt acggaggtag tcgcggttcc gctcgtgcgg cggaagctcg agcgacacac
     1081 gctcgaccag ctccaggccg tagcctttga gcgcgacgta cttgcccatg ttgttcgtca
     1141 tcagccgcag ccggcggatg cccaaatcgt acaggatctg cgcgccgacg ccgtagtcgc
     1201 gatggtcggc ctcgaagccg agcaggaggt tggcctcgac ggtgtccttg tgatggcggt
     1261 cctgcagctc gtaggcgcgc agcttgttgg ccagtccgat gccgcggccc tcctggcgca
     1321 ggtagaggat cgcgccgcag ccctcggcgg cgatgcggtc catcgccgcg tgcagctgcg
     1381 gaccgcaatc gcaacgcgtc gagccgaaga cgtcgcccgt caggcactcg gagtggacgc
     1441 ggaccagcac cggctgatcg ggtttcggct gacccatgac cagcgccacg tgggtctggt
     1501 tctcgagctc gtagtggaag gcgtggatcc tccagtcacc gagcgccgtc ggcaaatccg
     1561 gcgacgcgac gcgcttgacc agacgttccg tgcgcatgcg gtagctgatg atctcggcga
     1621 tcgtgatcaa cttcaggccg tgccggcggg cgaagatccg cagctcgggc aaccgcgcca
     1681 tcgtgccgtc ggggttcatc acctcgcaga cgacggccgc cggccgcaaa ccgcccaggc
     1741 ggcacaggtc gaccgaggcc tcggtgtggc cggcgcgctt gagcacgccg ccgggccggg
     1801 cgagcaacgg gaagacgtgt cccgggcgcg acaggtcgga cggcttggtc gcgggatcga
     1861 ccgccttacg gatcgtcgtc gcccgatccg cggccgagat cccggtcgtg atgtcgtgtc
     1921 gcgcctcgat cgagacgcag aacgccgttc cgcggcgggc ggtgttggtc ttgtcctgga
     1981 ccatcagcgg gatctcgagc gaatcgaggc gatcctgggt catcggcatg cagatcaagc
     2041 cgcgcccgtg cgtcgccatg aagttgatcg tctccggcgt gacgaactcg gcggcgcaaa
     2101 ccagatcgcc ctcgttctcg cgatcctcgt cgtcgatgac gatgatcatc ctgccgttgc
     2161 gcaggtccgc gatgacctct tcgatgcggt tgaacccggt ctccgacatg agcttcaccc
     2221 gtccgtccgg cgttgtgcga tcagcctttc aagatacctc gccagcagat caacctcaaa
     2281 gttcatcgtc tcgccagccc gaaagcggcc cagagtggtc cattcgagcg tgtgtgggac
     2341 cagcgcgacc tcgaagccgc cgcgaccgac ccccgcgacc gtcagcgaga cgccctgcag
     2401 cgcgaccgac cccttgtacg gtacgtacgg cgccagggcg gccggcaggt cgatgcgcat
     2461 ccggtaatcg tcgccccgac gtcgaaccgc tcggacccgc accgtgccgt cgacgtggcc
     2521 caggaccagg tgcccgccga cgcggtcgcc caggcgcagg gagcgctcga ggttgacggg
     2581 atctcccgga cgcaggcgtc cgagcgtcgt ccgcgccagc gtctcggcga cgacgtcggc
     2641 gaggaaccgg tcggtccgac gctccgcgac ggtcagacag acgccgtcga cggcgatgct
     2701 ctcgccgtcg accagctcgg ccgcgggcag gctcgactcg ataccgatcc gcgtcgcgcc
     2761 gtggcgtggg gcgacgtcgg tgacccggcc gaccgcctcg atcagcccgc tgaacatctc
     2821 atcgctcccg cccgggtcgt gccggaccga ccagccgccc caacaggagc aggtcgtcgc
     2881 ccagcgggac gatctgctct cgcgcgacgc gccaggcctc gtccggcgcc gcggccgcgt
     2941 ccccgtcgag gagcggccgc gcgccgcgcg caccgatcat gcgcgcggaa gtgaacaacg
     3001 cgacgcgctg agcgagcccg ctggcgacga acgatgccag cgtttgtcca ccaccttcga
     3061 cgagcaccga ttggatcccg cgggcggcca gctgctcgag cgcccggcgg acgaagccgg
     3121 cgtcgtcacc cgccgacagc tcgacgacct cggcgctcgc gagacgcgcc gcgcgagagc
     3181 cgtcggcgcc ggccggcgtg aaaaccagcg tgcgtccggc cgagctcgtc gactcgtcga
     3241 agaccgccgc ccggggatcg agatcgagac tgcgcgagaa gaccgcgcgc cgacgcgcag
     3301 cctcgcggcc gccgagccgt acggtcagcc gcgggtcgtc acggcgaacg gttcccacgc
     3361 cgaccagcac cgcgtcgtgt cgccagcgca ggcggtgcgc gaaccgccgg gcctcgggtc
     3421 cgctgatcca gcgcgagcag ccgtcgcgtg ccgccagcat gccgtcggcc gagatcgcgg
     3481 ccttgagcgt gaccatcggc agcccccgct cgtgccagcg gatgaacggc tcgttcaacc
     3541 gacgtgcttc gtcctcgagc aagccgaccc ggacctcgat tccggcgcgg cgcagctgct
     3601 cgaagccgcg accgttgacc agcgggttgg gatcctgcat cgacgccagc acccgcgcca
     3661 cgcccgacgc gatcagcgcc tcactgcagg gcagggtacg tccgacatgg gcgcacggct
     3721 cgagattgac gaccagcgtc gccccgcgcg cctcgccacc ggcctcccgc accgcgatcg
     3781 cctcggcgtg cggctcaccg ggcgcgcggt ggaaaccgcg gccgacgacg cggtcctgct
     3841 tgaccagaac gcagccgacg agtggattgg gcgaggtctg gccgtcggcc agcgcggcca
     3901 gcgccagcgc ctgggccatc cagtcgcgct cgcggccggt ccagtcccgg cccgcgctca
     3961 ccgctcgccg cccagcaggc cgtcgacgaa ggcgtcggga tcgaactcga gcaggtcgtc
     4021 gaccgcttcg ccgacgccga cgtagcgaat cggtacgccg agccggcgat ggatcgcgag
     4081 cgcgaccccg cccttggccg ttccgtcgag cttggtcagc accacgccgc tcagctcggc
     4141 cgcgcgggtg aattgctcgg cttgggccag cccgttctgc ccggtggtgg cgtcgatgac
     4201 gagcagcacc tcgtggggcg cccccgggat ctcgcgtgcc gccacgcgcg ccagcttgcc
     4261 gagctcggcc atcaggttgg tcttggtgtg cagccggccg gcggggtcga cgatcaccgt
     4321 ctcgaccccc cggccacgcg ccgcggccag cgcgtcgaag acgaccgccg ccggatcggc
     4381 cttctcgcgg tgcttgatca gctcggcatc cgcgcgctcg acccaccgtt cgagctgatc
     4441 gatcgccgcc gcacggaagg tgtcggcggc ggcgacgatc acccgacgcc ccgcgcgccg
     4501 ctcccgcgcg gccagcttgc cgaccgtcgt cgtcttgcct ccgccgttga cgccgacgac
     4561 gaagatcacg tgcggcgtgg tgccggccgc tgccggccgt cgatcgggga gcgctcctcg
     4621 cagcacgtcg cgcaccgcgc cggtcagccc cgcgccgtcg agcgacccgg ccgcggcacg
     4681 cgcgcggatc tcgtcgagga cctgcgcggt cagctcgggc cccaggtccg ccgccagcat
     4741 gctggcctcg agctcgtcga tcgcctcctc gtcgacttcg cgcgagccga cgacgctctg
     4801 caacgtggag gcgatggtgg cgcgcgtgcg ggccagtccg ttgcgcagct tgccgagaac
     4861 cgtcagtgcc atcgcttcag ttcatcattt tcagaatatc gtcggcttgc gccgcgctgt
     4921 tgctgccggg agcgacctcg atcgcccgtt ccaggctctc ccgggcgcgc gcccgatcgc
     4981 ccagccgaaa gtagacgaat ccgagccggt agtggaactc gcccacgttg cggtagtccg
     5041 gacgcgcgac ctcgtactcg cgcgcggcct cgtcgatccg gccaacccgg tcgagcaacg
     5101 acgccagctc gtagtgcgcc ttgtagaatt tcgggttgag ctcgaccgcg gtgcgcagct
     5161 cggagatcgc ctcctcgtcg cgaccctggg cgacgtaggc cagtgcgaga ttcaggtaga
     5221 ccttctccgg cgtcgggtag gccgggtcct cgagcgcctc gcgaaactgg tactcggcgt
     5281 cgtcgatccg acccatctcg gtgtacaccg cgccgaggaa gttgcgtgcg tcggtcaaga
     5341 acgggtcgtg ctcgagcgcg gtctcgaacg cctc
//
LOCUS       DASPGN010000041         4212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_593722, whole genome shotgun sequence.
ACCESSION   DASPGN010000041 DASPGN010000000
VERSION     DASPGN010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593722"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1221)
                     /gene="prfA"
                     /locus_tag="VD788_01265"
     CDS             complement(148..1221)
                     /gene="prfA"
                     /locus_tag="VD788_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014457754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXV74920.1"
                     /translation="MKDKLDELERRSDELSGALANPGITSDRERYRSTSRAYAELGPI
                     VESYAAYRRAVAELDGARELLRSADDAEMRAMAADEVKRLEQRLDQIEQRLRVAMLPS
                     DPNDAKNVVLEIRAGTGGDEATLFAAELFRMYRRYAEEVGWKVDTTSLSVSEVGGIKE
                     VIALVEGERVYSRLKYESGVHRVQRVPETEAQGRIHTSAVTVAVMPEADEVEVDIDEK
                     ELRIDTFCSSGPGGQSVNTTQSAVRITHLPTNIVVQCQDEKSWHKNKARAMTVLRSRI
                     YDRMLDEQRRAIAAERKGMVGTGDRSEKIRTYNFPQNRVTDHRIGLTLHNLPVVMNGA
                     VGEILDALSAHDQAEKLKQEVGR"
     gene            complement(1262..1468)
                     /gene="rpmE"
                     /locus_tag="VD788_01270"
     CDS             complement(1262..1468)
                     /gene="rpmE"
                     /locus_tag="VD788_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HXV74921.1"
                     /translation="MKKGIHPDYNEVEVHCACGNSFKTRSTVESLRVEICSECHPFFT
                     GKQKLVDSAGRVERFQRRYAKPGS"
     gene            complement(1568..2821)
                     /gene="rho"
                     /locus_tag="VD788_01275"
     CDS             complement(1568..2821)
                     /gene="rho"
                     /locus_tag="VD788_01275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXV74922.1"
                     /translation="MRIKELKAMEVGELIKIAGDLDVAGAGNLPYRELLYKVIQASAS
                     KNGSIIAEGVLEKLNDGYGFLRAPEASYLPGPDDIYVSPSQIRRFGLLTGDTVIGQIR
                     LPKPGERYFALGRVDEVNGSGPDASRKRLSFDKLTPLYPQERIRMEVTGDENISGRVM
                     DLMTPLGKGQRGLIVSPPRTGKTMLLQAIANAVAVNHPEVALIMLLIDERPEEVTDME
                     RTVKGEVISSTFDEPATRHVQVAEMVMEKAKRLVEAGVDVILLLDSITRLARAYNAVI
                     PASGRVLSGGLDANALQKPKRFFGQARNVEEGGSLTILATALIDTGSRMDDVIFEEFK
                     GTGNMEINLDRKLADRRVFPAVDLLRSGTRKEELLLSKLELDRTWVLRKVLQQMSPVE
                     AMELLRDKMLKTASNAEFLSSMASN"
     gene            complement(2818..3423)
                     /gene="yihA"
                     /locus_tag="VD788_01280"
     CDS             complement(2818..3423)
                     /gene="yihA"
                     /locus_tag="VD788_01280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HXV74923.1"
                     /translation="MKITSCRFVCSAYGPRDEPSDGAPEVVFLGRSNVGKSSLINRLL
                     ESRGLARTSSRPGRTQSVNFYRVNDAYYFVDLPGYGYARVPEAVRRNWQPMIEGYLGR
                     HRHAIGLAIVAVDARHRPRRLDLTMRDWLEAESIDYLVVATKADKLSGTGRVLARRSL
                     AREMDGSPVGGPVLFSAETGLGNKEVWRHLDRTLLQEKGNS"
     gene            complement(3459..>4212)
                     /locus_tag="VD788_01285"
     CDS             complement(3459..>4212)
                     /locus_tag="VD788_01285"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HXV74924.1"
                     /translation="ICRKLARKHVAGELESPVVVTPVSVVELLGVPRYHRPSEKPRPP
                     AVGAATGLAWTEVGGEILTIEATRMRGRGTTTLTGQLGEVMQESAQAALSYIRTRAEA
                     LDIDPGFHRKYDLHVHIPEGAIPKDGPSAGIALATALASLLSDEAVRGEVAMTGEITL
                     RGNVLPIGGVKEKVLAAYRYGFKTIILPKDNEKDLSEIPEDIASQLEFRLVENMDEVL
                     GAALVKGPAALGLPASEAKDSLDEASDGSVTH"
BASE COUNT          636 a         1486 c         1332 g          758 t
ORIGIN      
        1 ggtcgagcga atcgtcgcgt cggacgagca gcgccccgcg gtcggtgccg agcagctccg
       61 ccatcaacca ctccgcctcg agccgcggtc gagcgacgcc ggcctcggcc agcgccgtcg
      121 cggcctgggc cagtgcccgt ccgatcgtca gcggccgacc tcttgcttga gcttttccgc
      181 ctggtcgtgt gcggacaacg cgtcgagaat ctcgccgacg gccccgttca tcaccaccgg
      241 caggttgtgg agcgtcagtc cgatgcgatg atcggtcact cggttctgcg ggaagttgta
      301 ggtgcggatc ttctcgctgc ggtcacctgt cccgaccatc cccttgcgct cggcggcaat
      361 cgcgcgccgc tgttcgtcga gcatccggtc gtagatccgc gagcgcagca cggtcatcgc
      421 acgggccttg ttcttgtgcc acgacttttc gtcctgacac tgcacgacga tgttggtcgg
      481 caggtgggtg atgcggaccg ccgattgcgt cgtgttcacg ctctggccgc ccggtcccga
      541 cgagcagaag gtgtcgatcc gcagctcctt ctcgtcgatg tcgacctcga cctcgtcggc
      601 ctcgggcatc accgccacgg tgacggccga ggtgtggatt cgcccctgcg cctcggtctc
      661 gggcacgcgt tgcacgcggt gcacgccgct ctcgtacttg aggcggctgt agacccgctc
      721 gccctcgacc aacgcgatca cctctttgat accgccgacc tcgctgacgg agaggctggt
      781 ggtatcgacc ttccagccga cctcctcggc gtagcgccgg tacatgcgaa acagctcggc
      841 ggcgaacagc gtcgcctcat cgccgcccgt gccggcgcgg atctcgagca cgacgttctt
      901 ggcgtcgttg gggtcactcg gcagcatcgc gacgcgcaac cgctgttcga tctggtcgag
      961 ccgttgctcg agccgcttga cctcgtcggc ggccatcgcg cgcatctcgg cgtcgtcggc
     1021 cgagcgcagc agctcgcggg cgccgtcgag ctcggcgacg gcgcggcggt aggcggcgta
     1081 gctttcgacg atcggcccga gctcggcgta ggcgcgcgag gtggagcggt agcgctcccg
     1141 gtcggaggtg attccggggt tggccagcgc tccggacagc tcgtcggacc gacgctcgag
     1201 ctcgtcgagc ttatccttca aacgggggtc gagcgatccg cccatcggtc aaccccacgg
     1261 gtcaggagcc cggcttggcg tagcgccgct ggaaccgctc cacccgtccg gcggagtcga
     1321 cgagcttctg cttgccggtg aagaacgggt ggcattccga gcagatctcg acgcgcaacg
     1381 actcgaccgt cgaacgcgtc ttgaacgaat tgccgcaggc gcaatggacc tcgacctcgt
     1441 tgtagtcggg gtggatccct tttttcatgg cgagccctcg aaacccttgc gatcgaacgt
     1501 ggggaaggac ggtccgctcc cgttaaacga aggattatac gatgcggagg cgccgtgcgc
     1561 caaccggcta gttgctggcc atgctcgaca ggaattccgc gttggaggcg gtcttcagca
     1621 tcttgtcccg caacaactcc atcgcctcga ccggcgacat ctgctgcaac accttgcgca
     1681 gcacccaggt acgatcgagc tcgagcttgc tcaacagcag ctcttccttg cgggttcccg
     1741 agcgcaacag atcgaccgcc gggaataccc gccggtcggc gagcttgcgg tcgaggttga
     1801 tctccatgtt gcccgtcccc ttgaactcct cgaagatcac gtcgtccatg cgggagccgg
     1861 tgtcgatcag cgccgtggcc aggatcgtca acgacccccc ctcctcgacg ttgcgcgcct
     1921 gaccgaagaa gcgcttcggc ttctgcagcg cgttggcgtc gaggccgccc gacagcacgc
     1981 gtcccgaagc cggaatcacc gcattgtacg cgcgcgccag ccgcgtgatc gagtcgagca
     2041 acaggatcac gtcgacgccg gcttcgacca gccgcttggc cttctccatc accatttcgg
     2101 cgacctgcac gtgccgcgtc gccggctcgt cgaaggtcga cgagatcacc tcacccttga
     2161 ccgtgcgctc catgtcggtg acttcctcgg ggcgctcgtc gatcaacagc atgatcaacg
     2221 caacctcagg gtggttgacc gccacggcgt tggcgatcgc ttgcagcagc atcgtcttgc
     2281 cggttcgcgg gggggagacg atcaggccgc gctgaccctt gcccagcggc gtcatcaggt
     2341 ccatcacgcg ccccgagatg ttctcgtcgc cggtgacctc catcctgatc cgctcctgcg
     2401 ggtagagcgg ggtcagcttg tcgaagctga gccttttgcg cgaggcgtcg ggccccgagc
     2461 cgttcacctc gtcgacccgt cccagcgcga aatagcgctc acccggcttg ggcagacgga
     2521 tctggccgat gacggtgtcg cccgtcagca ggccgaagcg ccgaatctgc gacggcgaga
     2581 cgtagatgtc gtccggcccc ggcaggtagc tggcctccgg cgcacgcagg aacccgtagc
     2641 cgtcgttcag cttttcgagc acgccctcgg cgatgatcga cccgttcttc gaggcgctgg
     2701 cctggatcac cttgtacaac agctcgcgat agggcaggtt gcccgcgccg gccacgtcca
     2761 gatcgcccgc gatcttgatc agctctccga cctccatcgc cttcaattcc ttgattctca
     2821 tgagttgccc ttttcctgca gcaatgtccg gtcgagatga cgccacacct ctttgttccc
     2881 cagaccggtc tcggccgaaa acagcaccgg cccgccgacc ggcgatccgt ccatctcacg
     2941 cgccagcgag cgtcgcgcga gcacccggcc ggtgcccgac agcttgtcgg ctttggtcgc
     3001 caccaccagg tagtcgatcg actcggcctc caaccagtcc cgcatcgtca ggtcgagccg
     3061 gcggggtcga tggcgggcat cgacggcgac gatcgccagc ccgatcgcgt ggcgatggcg
     3121 ccccaagtaa ccctcgatca tcggctgcca gttacgccgc accgcctccg gcacgcgcgc
     3181 gtagccgtac ccgggcaggt cgacgaaata gtacgcatcg ttgacacgat agaaattcac
     3241 gctctgtgtc cgcccgggac gggacgacgt ccgcgccagc ccccggctct cgagcagtcg
     3301 attgatcagt gacgacttgc cgacgttcga tctgccgaga aacaccactt cgggtgcccc
     3361 gtccgacggc tcgtctcgcg ggccgtacgc cgaacagacg aaacggcacg atgtgatctt
     3421 cacgggcctc gcgcaatccg atctcgccga gcttcgtttc agtgcgtgac cgatccgtcg
     3481 gatgcctcgt cgagcgagtc tttggcttcg ctggccggca ggccgagggc ggccggtccc
     3541 ttgaccagcg ccgcacccag cacctcgtcc atgttctcga cgaggcggaa ctccaactgg
     3601 ctcgcgatgt cctcgggaat ctcggacagg tccttctcgt tgtccttcgg caggatgatc
     3661 gtcttgaagc cgtagcggta ggccgccagc accttctcct tgacgccgcc gatcggcagc
     3721 acgttgccgc gcagcgtgat ctcaccggtc atcgccacct cgccgcggac ggcctcgtcg
     3781 ctcaacagcg aggcgagcgc ggtggcgagc gcgatgcccg ccgacggccc gtccttgggg
     3841 atcgcgcctt ccgggatatg cacgtgcaga tcgtatttgc ggtggaaccc cggatcgatg
     3901 tcgagcgcct cggcgcgggt ccggatgtag gacagcgcgg cctgcgccga ttcctgcatc
     3961 acctcgccga gctggccggt cagcgtcgtc gtgccgcgcc cgcgcatgcg cgtggcctcg
     4021 atggtcagga tctcgccgcc gacctcggtc caggccagcc ccgtggccgc ccccaccgcc
     4081 ggcgggcgcg gcttctcgct cggccggtgg tagcgcggca ccccgagcag ctcgaccacc
     4141 gagaccggcg tgacgacgac cggcgactcg agctccccgg ccacgtgctt gcgcgccagc
     4201 ttgcggcaga tc
//
LOCUS       DASPGN010000042         7092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_711960, whole genome shotgun sequence.
ACCESSION   DASPGN010000042 DASPGN010000000
VERSION     DASPGN010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7092
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711960"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="VD788_01290"
     CDS             complement(<1..976)
                     /locus_tag="VD788_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXV74925.1"
                     /translation="MSPPAARRSRRMRSIALTVICCLAASPVAIAEEPQPEPGVTVRY
                     DDGLLVETGDGNFSTRINWRAQFRLTDLSSDELVGEEDGVEEESGFLIRRARFKMKGH
                     AYRPWLKFKFEYGIAASVMLTLLFDIEQSEELALWVGQYKVPYNRERVDSSGEQQFVD
                     RSIVNTPFTIDRQSGLTLTGRLFAGKRTDSSYAVGWYTGTGRGGELDNEQQGMYVGRW
                     QWNFLGRLLPFSQSDIARYPEGRGSLALAAASNTGRFTAFSSSGGGQLPGFDETDEPG
                     RYRLDQWMAELAYMRRGLSLQSEYHFKKIDDRVAQQITELEGWYGQVGY"
     gene            complement(1131..1679)
                     /locus_tag="VD788_01295"
     CDS             complement(1131..1679)
                     /locus_tag="VD788_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74926.1"
                     /translation="MDGVIGVLGAAWGAAGVIAILSFAVYRLLPRAIEAFEMGLTPVQ
                     WLITVAVCLAMAYSEGYRGFQLHFSPRTAARVRYLRDRPHGLRALFAPLFAMGFFHAT
                     RRTRWTAYGITCAVIFFVVLVQRLDQPWRGIVDAGVVVGLSWGVLSFAGFVIRALTRP
                     TYPVSPEVSAERRESPTTGSSH"
     gene            complement(1690..2580)
                     /locus_tag="VD788_01300"
     CDS             complement(1690..2580)
                     /locus_tag="VD788_01300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV74927.1"
                     /translation="MRIDTIDLQFEGTPQVIAAFLLRGAGGPVLIETGPGSTLATLHR
                     GLAALGVTAGDVRHVLLTHIHLDHAGAAGWWARHGATVYVHPAGAPHLIDPSKLIRSA
                     ERIYGDRMESLWGEILPAPAERVVALADGATIEVEGLSISALATPGHAAHHHVFRIGS
                     VAFTGDAAGIRLPGSEWIDLPAPPPEFDREAWRGSLDRLRQAGLTEIHRTHFGRCSTV
                     AADLAAFEAVMERGTEAIRELLEQGVDRDEMVRRFGAQMRARARELGIDGRALRAYEL
                     ANPRSMSVDGITRYWRKRAA"
     gene            2823..3728
                     /locus_tag="VD788_01305"
     CDS             2823..3728
                     /locus_tag="VD788_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV74928.1"
                     /translation="MVRTNQPGSAPAALIDVTKRYGNVVALDRLSLSIDRGRVTAVLG
                     PNGAGKTTAVRLMLGAQRPTSGRVELFGRSPADAESRMRVGAMLQVAKVPETLRVAEH
                     VALFSSYYPKRVPLDEVLEVVGLAGLADRAYGKLSGGEQRRLAFALALCGDPEMLVLD
                     EPTVGMDVEARRSFWERVRALADDGRTILLTTHYLEEADALADRIVVLNHGRIVADGS
                     AGEIKARVTARRIRCRSRLGLDEIRRLDRVELARSDRQALEIFTSSPESVVRELLARD
                     PTLTDLEVTSAALEDAFLALTRGAA"
     gene            3725..4540
                     /locus_tag="VD788_01310"
     CDS             3725..4540
                     /locus_tag="VD788_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV74929.1"
                     /translation="MMTQLELAIAADDAPRPRRSLARIFWLEAKLEFLKTWRVPAFTL
                     PAIGFPAVFYLLFGVAMKFGEGSPIHMATYLLATYGCFGVIGAALFGFGVGVATERGQ
                     GWMLTKRVSPMPIAAYFTAKLAMSLVFGAIIIALLSALGVLFGGVRLPLATWVPLIAI
                     LAGGALPFCALGLLIGYLVGPNSAPGVVNLIYLPLAFASGLWIPIVALPGIFRTLAHA
                     LPPYHYSQLALKTVGFDVGEPAWVHLVYLVVFTAVALGLARLAYRRDEGKTYG"
     gene            4565..5662
                     /locus_tag="VD788_01315"
     CDS             4565..5662
                     /locus_tag="VD788_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXV74930.1"
                     /translation="MRLLPSDPDLGWTPYAWLVYLPIFYVRPILAGAGVVEWAATIVA
                     TAIFFVLYFRGYWYRGRVLLPYVIGLASLGAVFSPFNPGASVFFVYAAAFIGFAGSPR
                     SGVRVLSMLLVFVAAEAWLLDVEPSGWIPGLVFSALIGGVNIHYAEAKRRNARLGLAH
                     EEVERTATAAERERIARDLHDLLGHSLSVITLKSELAGKLIDRDPERARREITEIEQI
                     SRGAMAEVRKVVTGFRARQLGVELTHARLALASADVDLEVESSPYDLSPAQESVLALA
                     LREAVTNVVRHAGATRCTVRMRQERGEFRLVVQDDGRGGNASPGSGLRGMAERVEALG
                     GTLERDGSNGMRLTVSLPLEPPRKVLGAGGR"
     gene            5659..6261
                     /locus_tag="VD788_01320"
     CDS             5659..6261
                     /locus_tag="VD788_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV74931.1"
                     /translation="MIRILIAEDQALVLGALGALLEIERDIEVVGRAGDGRQALELAE
                     RLAPDVVLTDIEMPRMTGLELVAAMRERRLAARAIIVTTFARPGYLRRALEAGAAGYL
                     LKDSPAEELAAAVRRVHAGGRAIDPGLAAEAWTEPDPLTARERQVLRLAGEGWTNARI
                     SKELHLSEGTVRNYLSDAIGKIGAANRVEAARIAQEKGWL"
     gene            6258..7022
                     /locus_tag="VD788_01325"
     CDS             6258..7022
                     /locus_tag="VD788_01325"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HXV74932.1"
                     /translation="MRALATAAAIFATCAAGESGTVVDVVDGDTLRLSSETGTSTVRL
                     LGVDTPELGRADHTAEFLAKEAHAFTRALVDGRRVRLERDATGDTVDRYGRALGYVFL
                     DDGRLLNALILSEGYGQAYTRFPFSRIDEFRRLEREAREAGRGLWDPRRVPTLAAADA
                     LAHAGRTATVCDRVASTRYLRSASGEPTFLNLARPYPDQPLTVVIWGADRPNFDEPET
                     RLRDRTICVTGKIRRFGGHAEIAVNDPSQIVVGPPS"
BASE COUNT         1078 a         2462 c         2470 g         1082 t
ORIGIN      
        1 aatagccgac ctggccgtac cagccctcga gctcggtgat ctgctgggcc acgcggtcgt
       61 cgatcttctt gaagtggtac tcgctctgca gcgacagacc gcgacgcatg taggccaact
      121 cggccatcca ctggtcgagg cggtagcgac ccggttcgtc ggtttcgtcg aatcccggca
      181 gctggccgcc accggaggaa gagaaggcgg tgaaacggcc ggtgttggat gccgccgcca
      241 acgccaacga gccgcgcccc tcggggtatc gcgcgatgtc ggactgggag aacggtaaga
      301 gccgcccgag gaagttccac tgccagcggc cgacgtacat cccttgctgt tcgttgtcga
      361 gttcgccacc gcgaccggtg ccggtatacc agccgaccgc gtagctcgaa tcggttcgct
      421 tgccggcgaa cagccgtccg gtcaacgtca gccccgattg ccggtcgatg gtgaacgggg
      481 tgttgacgat cgaccggtcg acgaactgct gctcgcccga cgagtcgacg cgctcgcggt
      541 tgtacgggac cttgtactgg ccgacccaca gcgccagctc ctcggactgc tcgatgtcga
      601 agagcagcgt caacatcacg ctggccgcga tgccgtactc gaacttgaac ttgagccatg
      661 ggcggtaggc gtggcctttc atcttgaaac gcgcgcgccg gatgaggaac ccgctctctt
      721 cctcgacgcc gtcctcctca ccgaccagct cgtcgctcga cagatcggtc aggcggaact
      781 gcgcccgcca gttgatccgc gtcgagaagt tgccgtcacc ggtctcgacc agcaggccgt
      841 cgtcgtagcg tacggtcaca cccggctcgg gctgcggctc ctcggcgatc gccacgggcg
      901 acgctgcgag gcagcagatc accgtcagcg cgatcgagcg catccgtctc gagcgacggg
      961 cggcgggagg tgacatcgaa tcctctccag gtcggtccag gcaaagggac cgattggagc
     1021 ggacgcgaat cagacttctg tgaagactct gttaaggatc ggtaaacttt gcatcgcgaa
     1081 cgtcgcacga ctcggcgacg acgaccgccg gtcgaggaga gccggatcct ctagtgcgac
     1141 gagcccgtgg taggcgactc ccggcgctcg gccgacacct cgggcgagac gggataggtc
     1201 ggccgtgtca gcgcgcggat cacgaacccg gcgaacgaca acacgcccca gctcagaccg
     1261 accaccaccc cggcgtcgac gatgccacgc cacggctgat cgagtcgctg gaccagcacg
     1321 acgaagaaga tgacggcgca ggtgatgccg taggccgtcc accgggtcct gcgcgtcgcg
     1381 tggaaaaaac ccatcgcgaa cagcggtgcg aacagcgcgc gcaagccgtg cgggcggtcg
     1441 cgcaggtagc gtacgcgcgc cgccgtgcgc ggggagaagt gcagctggaa cccgcggtag
     1501 ccctcgctgt aggccatcgc caggcagacc gcgacggtga tcagccactg gaccggcgtc
     1561 agtcccatct cgaacgcctc gatcgcccgc ggcagcagcc ggtagaccgc gaacgacagg
     1621 atggcgatca cgcccgcagc gccccacgcc gctccgagca cgccgatcac accgtccact
     1681 ccgcgctctc tacgcggcgc gcttgcgcca gtagcgggtg atgccatcga ccgacatcga
     1741 ccgcgggttg gccagctcgt aggcccgcag cgcccggccg tcgatgccca gttcgcgggc
     1801 ccgcgcgcgc atctgcgcgc cgaaacgccg caccatctcg tcgcgatcga caccctgctc
     1861 gagcagctcg cggatcgctt ccgtgccgcg ttccatcacc gcctcgaacg cagcgagatc
     1921 ggccgccacg gtcgagcacc ggccgaagtg ggtccggtgg atctcggtca gcccggcctg
     1981 ccgcagacga tcgagcgaac cgcgccaagc ttcgcgatcg aactcgggcg ggggagcggg
     2041 cagatcgatc cactcgctgc ccggcaggcg gattcccgcc gcatcgccgg tgaacgcgac
     2101 cgagccgatg cgaaagacgt ggtggtgcgc ggcatgaccc ggcgtggcga gcgccgagat
     2161 cgacaggccc tcgacctcga tcgtcgcccc gtcggcgagc gcgaccaccc gctcggccgg
     2221 cgcgggcagg atctcacccc acagcgattc catccgatcg ccgtagatcc gctcggcgct
     2281 ccggatcagc ttggacgggt cgatcaggtg cggcgctccg gccggatgca cgtagaccgt
     2341 cgctccgtgg cgcgcccacc agcccgccgc acccgcgtgg tcgaggtgga tgtgggtcag
     2401 cagcacgtga cgcacgtcgc cggccgttac gccgagcgcg gcgaggccgc ggtgcagcgt
     2461 ggcgagcgtc gagcccggac cggtctcgat cagcacggga ccaccggcgc cgcgcagcag
     2521 gaaagccgcg atcacctgcg gcgttccctc gaactggagg tcgatggtgt cgatacgcat
     2581 ctccccatcc tgccacgagc cggcggctta cggcgcgcgg acctgaacga cggttcgatc
     2641 tcgaccggaa tgcccggcgg cggccagccc cgtcgcagcg cacacctccg gcgaagaccg
     2701 acaagactag cagcaagccg ccgtgtgaca aacgtcagcg aaactggtga cggatgacaa
     2761 tggtcgagac ccgccgccgg gatcacaatc gcgtcgtacc gtaacgacgg gaggagatgc
     2821 agatggttcg aaccaatcaa cccgggtcgg cgccggcggc cctgatcgac gtgaccaagc
     2881 gctacggcaa cgtcgtcgcg ctcgaccgtc tcagtctttc gatcgaccgg ggacgcgtca
     2941 ccgcggtgct cggtcccaac ggcgccggca agacgacggc cgtacggctg atgctcggtg
     3001 cgcaacggcc caccagcggg cgagtcgagc tgttcgggcg ttcgccggcg gatgccgaga
     3061 gccggatgcg cgtcggagcg atgctgcagg tggccaaggt gccggagacg ctaagggtcg
     3121 ccgagcacgt cgccctgttc tcgagctatt acccgaaacg ggtcccgctc gacgaagtgc
     3181 tcgaggtcgt cgggctcgca gggctggccg atcgcgccta cggcaagctg tcgggcggcg
     3241 agcaacgccg gctggccttc gcgctcgcgc tgtgcggcga cccggagatg ctggtgctcg
     3301 acgagccgac cgtcggcatg gacgtcgagg cgcgtcgctc gttctgggaa cgcgtccgcg
     3361 cgctggccga tgacggccgc acgatcctgc tgacgaccca ctacctcgag gaggccgacg
     3421 cgctggccga ccgcatcgtc gtgctcaatc acggtcggat cgtcgccgac ggctcggccg
     3481 gcgagatcaa ggcgcgcgtg accgccaggc gaatccgctg ccgcagccgt ctggggctcg
     3541 acgagatccg gcgtctggac cgggtcgagc tggcacgcag cgaccgacag gcgctcgaga
     3601 tcttcacctc gagcccggag agcgtcgtgc gcgagctgtt ggcgcgcgac ccgacgctga
     3661 ccgatctcga ggtaacgagc gccgcattgg aagacgcctt cctggccttg acgagaggag
     3721 ccgcatgatg acccaactgg aactggcaat cgccgcagac gacgcaccgc gcccgcgccg
     3781 cagcctggcg cgcatctttt ggctggaagc gaagctcgag ttcctcaaga cgtggcgcgt
     3841 gccggcgttc accctgcccg cgatcggctt ccccgccgtc ttctacctgc tgttcggggt
     3901 cgcgatgaag ttcggcgagg ggagcccgat tcacatggcg acctatctgc tcgcgaccta
     3961 cggctgcttc ggcgtgatcg gcgccgcgct gttcggattc ggcgtcggcg tggcgaccga
     4021 gcgcggccag ggttggatgc tgaccaagcg ggtcagcccg atgccgatcg ccgcgtactt
     4081 caccgccaag ctggcgatga gtctggtctt cggcgcgatc atcatcgcgc tgctgtcggc
     4141 gctcggcgtg ctgttcggcg gagtccgtct gccgctcgcg acctgggtgc cgttgatcgc
     4201 gatcctcgcc ggcggcgcgc tgccgttctg cgcgctcggg ctgctgatcg gctatctcgt
     4261 cggtcccaac tcggcaccgg gggtcgtcaa cctgatctac ctgccgctgg cgttcgcctc
     4321 gggtctgtgg atcccgatcg tcgcgctgcc gggaatcttc cgcacgctgg cccacgcgct
     4381 accgccgtac cactattcgc agctcgcgtt gaagaccgtc ggcttcgacg tcggcgagcc
     4441 ggcgtgggtc cacctcgtct acctggtcgt cttcaccgcc gttgcgctcg gtctggcccg
     4501 actggcctac cgccgtgacg agggcaagac ctacggttga tcggttcgag gtagactctc
     4561 cccgatgcgt ctgctcccca gcgatcccga cctcggctgg accccgtacg cctggctggt
     4621 atacctgccg atcttctacg tgcggccgat cctcgccggg gcgggcgtgg tcgagtgggc
     4681 ggcgacgatc gtcgcgaccg cgatcttttt cgtgctctat ttccgcggct actggtaccg
     4741 gggtcgcgtg ctgttgccgt acgtcatcgg gttggcgtcg ctcggtgccg tcttctcgcc
     4801 gttcaatccg ggcgcctcgg tcttcttcgt ctatgccgcg gcgttcatcg gcttcgccgg
     4861 gtcgccgcga tccggcgtcc gggtgctgtc gatgctgctc gtgttcgtcg ccgccgaagc
     4921 ctggctgctc gacgtcgagc cctcgggctg gatccccggt ctggtcttct ccgccctgat
     4981 cggcggtgtc aacatccact acgccgaggc caagcgccgc aacgcacgcc tcggcttggc
     5041 gcacgaggag gtcgagcgga cggcgaccgc cgccgaacgc gagcggatcg cgcgcgatct
     5101 gcacgacctg ctcggccaca gcctgtcggt gatcacgctg aaatccgagc tggccggcaa
     5161 gctgatcgac cgcgaccccg agcgcgcgcg tcgcgagatc accgagatcg agcaaatctc
     5221 gcgcggggcg atggccgaag tgcgcaaggt ggtgacgggg ttccgcgcaa ggcagctcgg
     5281 cgtcgagttg acccacgccc ggctggcgtt ggcctccgcg gacgtcgacc tcgaggttga
     5341 aagctcgccc tacgacctgt cgcccgcgca ggagagcgtg ctggcgctcg cgctgcgcga
     5401 ggcggtgacc aacgtcgtgc gtcacgcggg ggcgacgcgc tgcacggtgc gcatgcggca
     5461 ggagcgcggc gagttccgcc tcgtcgtgca ggacgacgga cgtggcggca acgcgtcgcc
     5521 cggctccggc ctgcgcggga tggccgagcg ggtcgaggcg ctcggcggca ccctcgagcg
     5581 agacggatcg aacggcatgc gactgaccgt gagcttgccg ctcgagccgc cgcgcaaggt
     5641 gctcggcgcg ggcggccgat gatccgcatc ctgatcgccg aggaccaggc actggtgctc
     5701 ggtgcgctcg gcgcgttgct cgaaatcgaa cgtgacatcg aggtcgtcgg ccgcgcgggc
     5761 gacgggaggc aggcgctcga gctggccgaa cggctcgcgc ccgacgtcgt gctgaccgac
     5821 atcgagatgc cgcgcatgac cggtctcgag ttggtcgcgg cgatgcgcga gcgtcggctg
     5881 gcggcacgcg cgatcatcgt gacgaccttc gcgcgccccg gctacctgag gcgagcgctc
     5941 gaggccggtg ccgccggtta cctgctgaag gactcgccgg ccgaggagct ggcggcggcg
     6001 gtccgccgcg tacacgccgg cggccgcgcg atcgacccgg gcctcgccgc cgaggcctgg
     6061 accgagcccg acccgctgac ggcgcgcgag cgccaggtgc tgcgtctggc cggcgagggc
     6121 tggaccaacg cgcgcatctc caaggagttg cacctgagcg agggaacggt gcgcaactac
     6181 ctgtcggacg cgatcggcaa gatcggcgcg gccaaccgcg tggaggccgc acgcatcgcg
     6241 caggagaagg gctggttgtg agggcgctgg ccacggcggc cgcgatcttc gccacctgcg
     6301 ctgcgggcga gagcggaacc gtcgtcgacg tcgtcgacgg cgacacgctg cgcctgtcgt
     6361 cggagacggg cacgtcgacg gtgcgattgc tcggtgtcga cacgccggag ctcggccggg
     6421 cggaccacac ggcggagttc ctcgcgaagg aggcccacgc cttcacccgc gcgctggtcg
     6481 acggccggcg tgtgcggctc gaacgcgacg cgacgggtga caccgtcgat cgctacggcc
     6541 gggcgctggg ttacgtcttc ctcgacgacg gccgcctgct caacgcgctg atcctgtccg
     6601 aaggctacgg ccaggcctac acgcgcttcc cgttctcgcg gatcgacgag ttccggcgtc
     6661 tcgagcggga ggcgcgcgag gcgggacgcg gactgtggga tccgcgccgg gtgccgacgc
     6721 tcgctgcggc cgacgcgctg gcgcacgccg gccgaaccgc gaccgtctgc gaccgcgtgg
     6781 cgtcgacgcg ttacctgcgc tcggcgagcg gcgagccgac cttcctcaac ctggcacgcc
     6841 cgtatcccga ccagccgctg acggtggtga tctggggcgc cgatcggccc aacttcgacg
     6901 agcccgaaac gcggttgcgc gaccggacga tctgcgtgac gggcaagatc cgccggttcg
     6961 gcggtcatgc cgagatcgcc gtcaacgatc cctcccagat cgtcgtagga ccgccgagct
     7021 gacgacggtg gcccgtgcgt ttcagtcggc agccggcctg agcgccggcg tcgagtggtc
     7081 gggcggcgtc ac
//
LOCUS       DASPGN010000043         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_360676, whole genome shotgun sequence.
ACCESSION   DASPGN010000043 DASPGN010000000
VERSION     DASPGN010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360676"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..900
                     /locus_tag="VD788_01330"
     CDS             118..900
                     /locus_tag="VD788_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV74933.1"
                     /translation="MGVRVVPLGSGSRGNSTLVEADGSRVLVDAGLSGRTLVQRLDGI
                     GIAPQRLTAIVLSHEHSDHAMGAERLSKRFGVPLVCSRATLDALDRSHRHFADWVELR
                     DGEELDLDGVSLTHFPIPHDAAKPVGFVLRAGGVRIGIATDLGHATTLVVERLRGCEV
                     LMIESNHDERMLRDGPYPWQLKQRVASRMGHLSNGDAARLVRAVAGPDCRALVLAHLS
                     EKNNTPELARAAATRALAEAGANRVTMRVASSRRPTPPLELR"
     gene            914..>2161
                     /gene="purB"
                     /locus_tag="VD788_01335"
     CDS             914..>2161
                     /gene="purB"
                     /locus_tag="VD788_01335"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXV74934.1"
                     /translation="MIERYTRAEMGALWTERAKFDSWLEVELAVCEVRAERGEIPADE
                     LEQIRANASFDIARIEEIERTVAHDVIAFLTSVAERIGPAARHVHYGLTSSDVVDTAQ
                     GLRTVRAADLLLRGLDRLIAVLKSQAARHKDTLMVGRTHGIHAEPYTLGLKFAGWYTE
                     ALRNRERLRAAREGIRVGKLSGSVGTYAHLGPEVELLVMDRLGLRVEPVSTQVVPRDR
                     HAAFVASLGVLASSLDRITTEIRHLQRSDVREVEEPFAGGQKGSSSMPHKRNPVKSEN
                     VSGLARIVRSWVQAALEDVPLWHERDISHSSVERVMLPDVTILCDFMLHRVSDILERL
                     HVYPERMRQNMAITRGLIFSQAVLLALTRHGMGRDDAYAVVQDNAMRTWAGEGDFRDL
                     LAADERLRNVIGEEQLAQCFDPQR"
BASE COUNT          302 a          724 c          804 g          331 t
ORIGIN      
        1 cggcgaccgg atcgcgcagc tcgtggtgca gcagcttccg cgggtgcgcc tggtgcaggt
       61 cgacgagctg ccggcctcgg cgcgaggcgc cggcgggttc ggtcatacgg ggtcgtgatg
      121 ggcgtgcggg tcgttccact cggcagcggg tcgcgcggca actcgacgtt ggtcgaggcc
      181 gacggttccc gcgtgctggt cgatgcgggt ctttccgggc gcaccttggt ccaacggctc
      241 gacgggatcg gcatcgctcc ccagcggctg acggcgatcg tgctgtccca cgagcattcg
      301 gatcacgcga tgggggccga gcgactgtcc aagcgcttcg gcgtaccgct ggtctgctcg
      361 cgcgcgacgc tcgatgcgct cgaccgttcg caccggcact tcgccgactg ggtcgagctg
      421 cgcgacggcg aggagctcga tctggacggg gtctccttga ctcactttcc gatcccgcac
      481 gatgccgcca agccggtcgg cttcgtgctg cgcgccggcg gggtgcgcat cggcatcgcg
      541 accgatctgg gtcacgccac gacgctcgtc gtcgagcggc tgcgcggctg cgaggtgctg
      601 atgatcgagt cgaaccacga cgagcggatg ctgcgcgacg gcccctaccc gtggcagctc
      661 aagcaacggg tggccagtcg gatgggccac ctgtcgaacg gcgacgcggc gaggttggtt
      721 cgcgcggtgg ccgggccgga ctgccgcgcg ctggtgctcg cgcacctgtc ggagaagaac
      781 aacacgcccg agctggcccg cgccgccgcc acgcgcgcgc tggcggaagc gggagccaac
      841 cgcgtgacga tgcgcgtcgc gtcgtcgcgc cggccgactc cgccgctcga gctgcgatga
      901 agggagtcgc gtcgtgatcg agcggtacac gcgcgccgag atgggcgcgc tctggaccga
      961 gcgggcgaag ttcgacagct ggctcgaggt cgagctggcg gtctgcgagg tgcgggccga
     1021 gcgcggcgag attcccgccg acgagctgga gcagatccgc gccaacgcgt cgttcgacat
     1081 cgcccggatc gaggagatcg agcggacggt ggcccacgac gtcatcgcct tcctgacgag
     1141 cgtggccgag cggatcgggc cggcggcgcg tcacgtgcac tacgggctca cctcgtcgga
     1201 cgtggtcgac acggcgcagg ggctgcgcac ggtccgtgcc gccgatctgc tgctccgcgg
     1261 cctcgaccgg ttgatcgccg tgctcaagag ccaggcggcg cgccacaagg acacgctgat
     1321 ggtcggccga acccacggga ttcacgccga gccctacacg ctggggctca agttcgccgg
     1381 ctggtacacc gaggcgctgc gcaaccgcga gcgtctgcgc gcagcgcgcg aggggatccg
     1441 cgtcggcaag ctgtcgggct ccgtcggcac ctacgcccac ctcggtcccg aggtcgagct
     1501 gttggtgatg gaccggctcg gcctgcgtgt cgagccggtc tcgacgcagg tcgtgccccg
     1561 ggatcggcac gcggcgttcg tcgcgtcgct cggcgtgttg gcctcgtcgc tcgaccggat
     1621 cacgaccgag atccgtcacc tccagcgctc ggacgtgcgc gaggtggagg agccgttcgc
     1681 cggcggacag aagggctcgt cgtcgatgcc gcacaagcgc aacccggtca agagcgagaa
     1741 cgtgtcgggc ctggcgcgca tcgtgcgctc atgggtccag gccgcgctcg aggacgtgcc
     1801 gttgtggcac gagcgcgaca tctcgcactc gtcggtcgag cgggtgatgc tgcccgacgt
     1861 gacgatcctc tgcgatttca tgttgcaccg cgtcagcgac attctcgagc gactgcacgt
     1921 ctaccccgag cgaatgcggc agaacatggc gatcacgcgc ggcctgatct tctcgcaggc
     1981 ggtgctgctg gcgctgacgc ggcacggcat gggccgcgac gacgcctacg cggtcgtcca
     2041 ggacaacgcg atgcgcacgt gggccggcga gggcgacttc cgcgatctgt tggcggcgga
     2101 cgagcggctg cgcaatgtga tcggcgagga gcagctggca cagtgcttcg atccgcagcg
     2161 c
//
LOCUS       DASPGN010000044        10293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_244381, whole genome shotgun sequence.
ACCESSION   DASPGN010000044 DASPGN010000000
VERSION     DASPGN010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10293
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244381"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /gene="uppS"
                     /locus_tag="VD788_01340"
     CDS             complement(<1..656)
                     /gene="uppS"
                     /locus_tag="VD788_01340"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HXV74935.1"
                     /translation="MIDTAELAGSGDEERRLVSQLDLDRLPRHVAVIMDGNGRWAHDR
                     RLPRVAGHRAGIDAVREIVEVSARLRLEVLTLYAFSLENWKRPPAEVSTLMELLKEYV
                     RRELPRLHENDIRLRVIGRIDDLPDGPRGELDHALEQTRDNRGLQFNIALSYGGRAEI
                     VDACRRILRLGLPPERVDERLLASQLYTAGQPDPDLLIRTSGELRVSNFLLWQIAYAE
                     "
     gene            complement(769..1770)
                     /gene="galE"
                     /locus_tag="VD788_01345"
     CDS             complement(769..1770)
                     /gene="galE"
                     /locus_tag="VD788_01345"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HXV74936.1"
                     /translation="MNGKILVTGGAGYIGSHIVRMLAESGSEIVIVDDLSEGHAAAVP
                     GIPLVRADFADAAMLDSILGDGKVAAIVHMAAFCEVGQSVKDPASYYRNNLSRSLDLL
                     DAAVRHGVRDVVFSSTAAVYGEPDALPITEIHDQRPTNPYGETKLAVERALGWYYGAY
                     GLRSVSLRYFNAAGAHPSAEIGEDHANETHLIPRLLLGVLHGSEAVPIFGRDYPTPDG
                     TCVRDYIHVVDLARAHLLALEALATGRVGCEAFNLGNGAGFSVLDVIETVGRITGTRP
                     ATRDAERRAGDPASLVASSARIAARLGWRPSFDTLDEIVGSAWGWHRGHPRGYATNG"
     gene            complement(1767..2561)
                     /gene="truA"
                     /locus_tag="VD788_01350"
     CDS             complement(1767..2561)
                     /gene="truA"
                     /locus_tag="VD788_01350"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXV74937.1"
                     /translation="MTLRRIRLELSYDGTDFAGWQWQDGRRTVQGVVEQALRRLAGDR
                     VVRLRAAGRTDAGVHARAQVADCLIDSRLDDDTLARALGRMLPPDVRPRSVATVDRGF
                     SAMRQAVRKTYRYRVDRSRFGNPLIARFALHCPDPLDAQRIERTLAMLVGRRDWSGFA
                     DSRCRVENRVRDLTEATFREHDGDEASFHFTADGFLTYMVRNMVGTVLEIGRGRMPVE
                     VIGRMLASRDRRLGAATAPARGLCLWEVVYARRPAIPAERGGTSDE"
     gene            complement(2558..3484)
                     /locus_tag="VD788_01355"
     CDS             complement(2558..3484)
                     /locus_tag="VD788_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HXV74938.1"
                     /translation="MAARRLNGGRRRFLAPGDAIGVVATGFAVRPEKLDAGLARLHRW
                     GYRVVTGAHLRDRDGYLAGSDRGRGDDLAAMLRNTEPSAIWFARGGYGTARLLDRVPW
                     AALRRDPKLLIGYSDLTALFNAVVSRTASVCLYGPVVAELGDRASWHAPSLDALSRGR
                     PYRLRLRAGQVLAPGRAAGTLVGGNLTVLTHLLGTRYAPDFRGRVLFLEETGEETYRV
                     DRMLTHLRQSGALDRVAAVLVGAIDVPPRRRFPPDRSLAAVVEERLAPLGVPVLTGIP
                     AGHLRAKRTIPLGARAVIDTDELAIRFEPRRA"
     gene            complement(3557..3961)
                     /gene="rnpB"
                     /locus_tag="VD788_01360"
     ncRNA           complement(3557..3961)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VD788_01360"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3990..4634)
                     /locus_tag="VD788_01365"
     CDS             complement(3990..4634)
                     /locus_tag="VD788_01365"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HXV74939.1"
                     /translation="MRVLTVNPNHPRDDRLAEAVAALETGGLVALPTETLYGLAVDAF
                     DVAAVERVNRVKGKPAGSPMLLLISDPAQVKAVSPVVDDRFRELTELFWPGPLTLVVR
                     ARRDLPAAVTGGTGTVAVRVPGLALPRRLAGALGRPITGPSANRHRQPACRTALEVVE
                     VFGEVIDLVLDGGPTAAGKPSTILDISGPEPRILREGLLPASSLQPFIPELNAS"
     gene            4714..5511
                     /locus_tag="VD788_01370"
     CDS             4714..5511
                     /locus_tag="VD788_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXV74940.1"
                     /translation="MLGKRHPLIRRIRDLKRDPTLRRRERLYVAEGLHLALEAVRSSA
                     AIESFVVSPRLSADDEGRELLGRLRGSGARVEETSDETLESIQDARSPQPVVGLLRFP
                     ADAGAAVRSVLAAAELVLVLDSIQDPGNLGSIVRSADAAGCDACFVIGRSADPLHPRA
                     VRGAMGSVGRLWPRPAPSDELFGALAASGHRVVGSDPHDGAPYDAEDLTGRMALVLGG
                     EAAGISRTARGRLTRTVRIPMRAGIDSLSVAAAGAVLLFEAARQRRR"
     gene            complement(5496..7901)
                     /locus_tag="VD788_01375"
     CDS             complement(5496..7901)
                     /locus_tag="VD788_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXV74941.1"
                     /translation="MVQPPVALAAGLFERANATALALGGLLGLVLLLALSVLLLIRSL
                     ARRSRTEDVTRLVHALEELRSGSYAARVDLDPGSPLSVVADAIHRAGHDVKLRLAEAA
                     ADRERLQLVMQAFQDSAVLTIDLDGDIRDFTESAEQMLGWSADEARGRPSSMIFEEDA
                     FREFVPQLSRRATFDGGVISRSLLRRKNGSQFPAEVTVRPIRSKKGESTGFLMLLRDV
                     TRQAGLEQELRLSEERHRRLVESLTDGVAIVSRGTLVYVNPAFSAMTGLPIETLIGTR
                     LRDRVVTRDVLMIDARLEALELGGAGTDEMRCTLAGADDRPRADVRIHATSVEFEGGI
                     GVLLLVHDETAARRIETELRRNESQLDAVLEATRDGMLVLVDRGRGGVVQMTNRAFLR
                     IFGLREPQVLGQPEGRLIRALREREDGAAAVAELLASGTVEPNGRPVRLGGAAPKDLS
                     VATFALVDRDGKRIGRVVVCRDTTRQRDAERELQQHAEKLQLSNVELERALAELDRAN
                     RDLASRTEDLGKLNRELQRLDELRQKLLGNLSHELQAPLVAIRGYTEMTLKERLGPIT
                     AEQRKGLTLSLGNIDRLIGLIDSLTHAPDLAELKPTTFLLRELIEQTADLLRREMQAK
                     GIHFELSMSDDGARVQADRAKVEQVFINLLSNAIKFNKPGGSIEITVGPGEPRYLEVA
                     VRDSGKGIPAADLPRVFDRHYRVATTGGGESGRGLGLAIVREILSLHGCRVRAESEPG
                     AWTRVSFTLPRAPEGDREPDRRDAAEAGTPAAGDDEASASDRSTRAAPRPRFRVIRRY
                     RRR"
     gene            complement(7960..9279)
                     /locus_tag="VD788_01380"
     CDS             complement(7960..9279)
                     /locus_tag="VD788_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV74942.1"
                     /translation="MSPNGERTEPERWHYLKLRSVLYDRITGLPSFPVLIDRLRGWLE
                     LRRAIGVIHVEAENLSLVESLYGWQVFDRILERVAGVLTSGVRELCGADAALGLNGVA
                     GDRFALFVASRLDGGDVDTRYLAETAETLRRRLDRAFAGDDFAGLNPKLEFRLGHAML
                     SHDPFFRFERQLYTAIDQARSADHRRLGRQLRGHGDDLLRILRDAELRTVFQPVVDLR
                     SGATLGYEAFARGPVDTALEAPRMLFRVSDRLGVSRDLDSLCRKLAVEAAARLPRPGK
                     VFLNGLPDSFRAVPTAAAAWPERLRPLRDEPERWVLEFSERAADGDPEGFVSTLDRLK
                     RHGLAVSVEDIGTGFTSQAILEELRPDFLKLDVTLVRKIDQNLIKQELLRSLVRIATR
                     IGASVIAEGVETEQEARALTEAGARYGQGHLFARPTSRIEELRGRVP"
     gene            complement(9281..9697)
                     /locus_tag="VD788_01385"
     CDS             complement(9281..9697)
                     /locus_tag="VD788_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV74943.1"
                     /translation="MQPRPSIMVVDDQADIRELTALLLEGAGYDVVAKPSGADALRHL
                     VAHSCQLVLLDINMPEMDGWETLRLIRADDSLADLPVVMFSVKDRIHDRVLGMQEGAV
                     DYITKPFEVDQLLYRVRRVLEARRGGPRPPTSQPVA"
     gene            9820..>10293
                     /locus_tag="VD788_01390"
     CDS             9820..>10293
                     /locus_tag="VD788_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV74944.1"
                     /translation="MSKIPFSIALLALAAVAFAGAAAEKATDDWTQWGGPDHEFRATS
                     AGLAKQWSADGPRELWTRELGDGYSAILYQDGKLYTMYRSGDEEAVVCLNAKTGKTVW
                     EHRYRAAPAEGHVSQFGEGPRSTPLIAGGMIYTIGVAGDLYALDKSNGKPVWNHDL"
BASE COUNT         1543 a         3640 c         3616 g         1494 t
ORIGIN      
        1 atctcggcgt aggcgatctg ccacaacagg aaattggaga cgcgcagctc tccgctggtg
       61 cgtatcaaca ggtccgggtc gggttggccc gcggtgtaga gctggctggc cagcagccgc
      121 tcgtcgacgc gctcgggcgg caggcccaac cggaggatgc ggcgacaggc atcgacgatc
      181 tcggcccgcc cgccgtagga cagcgcgatg ttgaactgca atccgcggtt gtcccgcgtc
      241 tgttcgagcg cgtggtcgag ctcgccgcgc gggccgtcgg gcaggtcgtc gatccgcccg
      301 atcacccgca gccggatgtc gttctcgtgc agcctcggca gctcgcggcg cacgtactcc
      361 ttgagcagct ccatcagcgt cgagacctcg gccggcgggc gtttccagtt ctcgagcgaa
      421 aaggcgtaga gcgtcagcac ctcgagccgc agacgcgcgc tgacctcgac gatctcacgg
      481 acggcgtcga taccggcgcg atgcccggcg acacgcggta gacgccggtc gtgggcccag
      541 cggccgttgc cgtccatgat gacggcgacg tggcgtggca ggcggtcgag gtccaactgc
      601 gagaccagtc gtcgttcctc gtcgccggaa cccgccagtt ccgcagtgtc gatcatccgc
      661 tgcctaccgc gggtgcgaag tgcggccgtt cgatcggagc acccccgcct ccccgggcgc
      721 atactaatgc cgaaccgtcc gtccatcaac cacggtcggc gcggtcgctc agccgttcgt
      781 cgcgtagccg cgcgggtggc cgcggtgcca gccccatgcg ctgcccacga tctcgtcgag
      841 cgtgtcgaag ctcggccgcc atcccaatcg cgcggcgatc cgcgccgagg aagccacgag
      901 cgacgccggg tcaccggcgc ggcgctccgc atcgcgagtc gccggtcgcg tgccggtgat
      961 ccggccgacc gtctcgatca cgtcgagcac cgagaagccc gcgccgttgc cgaggttgaa
     1021 cgcctcgcag ccgacgcgac cggtcgcgag ggcctcgagc gccagcaggt gcgcccgcgc
     1081 caggtcgacg acgtggatgt agtcgcgcac gcaggttccg tcgggcgtcg ggtaatcgcg
     1141 cccgaagatc ggaaccgcct ccgacccgtg gagcaccccc aacaacagcc gcgggatcag
     1201 atgggtctcg ttggcgtggt cctcgccgat ttcggccgac gggtgtgcac cggcggcgtt
     1261 gaagtagcgc agcgagaccg agcgcagacc gtaggcgcca tagtaccagc cgagcgcccg
     1321 ttcgaccgcg agcttggtct ccccgtacgg gttcgtcggt cgttggtcgt gaatctcggt
     1381 gatcggcagc gcgtccggtt cgccgtagac cgccgcggtg ctcgagaaca ccacgtcgcg
     1441 cacgccgtgc cgtacggccg cgtcgagcag atcgagcgac cgcgagagat tgttgcgata
     1501 gtacgatgcg ggatccttga ccgattgacc gacctcgcag aaggcggcca tgtggacgat
     1561 cgcggcgacc ttcccgtcgc cgaggatcga atcgagcatc gccgcgtcgg cgaagtcggc
     1621 tcgcaccagc gggatccccg ggacggccgc ggcgtggccc tccgacaggt cgtcgacgat
     1681 gacgatctcg ctgccgctct ccgccagcat gcgcacgatg tgactaccga tgtagcccgc
     1741 cccgcccgtg accaggatct tgccgttcat tcgtcgctcg tgcctcccct ctcggccggg
     1801 atcgccggac ggcgagcata cacgacctcc cacagacaca gtccccgagc cggcgcggtc
     1861 gctgcgccga gccgccggtc gcgcgacgcg agcatgcgac cgatcacctc gaccggcatc
     1921 cggccacgac cgatctcgag cacggttccg accatgttgc gcaccatgta ggtcaggaag
     1981 ccgtcggcgg tgaagtggaa cgacgcctcg tcgccgtcgt gctcgcggaa cgtcgcctcg
     2041 gtcaggtccc gcacccggtt ctcgacccgg cagcgcgagt cggcgaaccc cgaccagtca
     2101 cgacgtccga ccagcatcgc gagcgtccgc tcgattcgct gggcgtcgag cggatcgggg
     2161 caatgcaggg cgaaccgcgc gatcaacgga ttgccgaagc gggagcggtc gacgcgatag
     2221 cggtaggtct tgcgcacggc ctgccgcatc gcgctgaacc cgcgatcgac cgtcgccacc
     2281 gagcgcggcc gcacgtccgg cgggagcatc cggcccagcg cgcgggccaa cgtgtcgtcg
     2341 tcgagccgac tgtcgatcag gcagtccgcg acctgcgcgc gcgcgtgaac cccggcgtcg
     2401 gtacgcccgg cggcgcggag ccgcacgacg cgatccccgg ccagtcgcct cagcgcctgc
     2461 tcgacgacgc cctggaccgt gcgccgcccg tcctgccact gccaaccggc gaaatcggta
     2521 ccgtcgtagc tcagttcgag ccggatccgc cggagcgtca tgcccgccgc ggctcgaaac
     2581 gaatggcgag ttcgtccgtg tcgatcacgg cgcgcgcgcc gagcgggatc gtccgcttgg
     2641 cgcggagatg accggccggg atgcccgtca gcacgggaac cccgagcggc gccagtcgct
     2701 cctcgacgac ggcggcgagc gagcgatcgg gcgggaaccg gcggcgcggt gggacgtcga
     2761 tcgcgccgac gagcaccgcc gcgacgcggt ccagcgcgcc ggactgccgc aggtgggtca
     2821 gcatgcggtc gacgcgatag gtctcctccc cggtctcctc gaggaacagc acccggccgc
     2881 ggaagtccgg tgcgtaacgc gtaccgagca gatgggtcag caccgtcagg ttgccgccga
     2941 cgagcgtgcc ggcggctcgg cccggagcga gcacctggcc ggcgcggagc cgcagccggt
     3001 agggcctgcc gcgtgacaac gcgtcgagcg acggggcgtg ccagctggcg cgatcgccca
     3061 gctcggcgac gaccggtccg tacagacaga cggaagcggt gcgggagacg acggcattga
     3121 acagtgcggt caggtcgctg tagccgatca gcagcttcgg atcgcgacgc agcgcggccc
     3181 acggcactcg gtcgagcagc cgcgccgtcc cgtagcctcc gcgggcgaac cagatcgccg
     3241 acggctcggt gttgcgcagc atcgcggcca ggtcgtcgcc gcggccgcgg tcggaaccgg
     3301 cgagataccc gtcgcggtcg cgcaggtgtg cgccggtgac gacgcggtag ccccagcggt
     3361 gaagccgagc gagcccggcg tcgagcttct cggggcggac ggcgaatccg gtggcgacca
     3421 cgccgatcgc gtcgcccggc gcgagaaaac gtcgcctacc gccgttgagc ctgcgagccg
     3481 ccacgccgaa caccgccacc gaagttgccg ccgggccgcc ccccggccaa ccggagggtg
     3541 cggcccgacg gctcgccgcg cgagccgcac gtaagccgaa tcctgtctcc ccgtgagggg
     3601 cggcgaccat tcgtctggga gcgggatcac tcccgccctc tagcgaccta cccgggagca
     3661 tcggccgggc cgacctcgag cgctccccta ttcggtcttg ctccgcgtgg ggtttaccga
     3721 tccaccggcg tcaccgccgg tgctggtgcg ctcttaccgc accgtttcac ccttgccttc
     3781 cgttgcccga gcccgtcgcg cgttggctcg cgacgcggca acggacggcg gtctgctctc
     3841 tgttgcactt tccgtcccgt caccgggcct gggcgttacc cagcacgctg ccctgtggag
     3901 ttcggacttt cctcttcggc gcgggtttcc ccgaccccga aacgatcgcc tgtccgcctc
     3961 gcacgacggc gtcattatag cggaggcgct caggaggcgt tgagctccgg gatgaacggc
     4021 tgcaacgacg aggccggtag cagaccctcg cgcaggattc gcggctcggg tcccgagatg
     4081 tcgaggatcg tcgacggctt gccggccgcg gtcgggccgc cgtcgagcac caggtcgatc
     4141 acctcgccga agacctcgac cacctcgagc gccgtgcgac acgccggctg acgatggcgg
     4201 ttcgcgctcg gaccggtgat cgggcggccg agcgctccgg ccagccgccg cggtagggcc
     4261 agacccggga cgcgcaccgc gaccgtcccc gtgccgccgg tgaccgccgc gggcaggtcg
     4321 cgacgggcgc ggacgacgag cgtcagcggc ccgggccaga acagctccgt cagctcgcga
     4381 aaacggtcgt cgacgacggg cgacaccgcc ttgacctgtg ccgggtcgga gatcagcaac
     4441 agcatcggcg agccggcggg cttacccttt acgcggttga cgcgctcgac ggccgcgacg
     4501 tcgaacgcat cgacggccag cccgtagagc gtctcggtcg gtagtgcgac gaggccgccg
     4561 gtctcgagcg cggcgacggc ttcggcgagt cggtcgtcgc gcgggtggtt cgggttgacg
     4621 gtgagaacgc gcaaatcgcc ggctccgtcg cttggtggac gcgcgggacg agtttaccat
     4681 cagaccgtcg accgccgaca aggagaacgg ccggtgctcg gaaaacgcca cccgctgatc
     4741 cggaggattc gcgacctgaa gcgcgatccg acgctgcggc gccgcgaacg gctctacgtc
     4801 gccgaagggc tgcacctcgc gctggaagcg gtgaggtcgt ccgccgcgat cgagtcgttc
     4861 gtcgtctccc cgcggctgtc ggccgacgac gaggggcgtg agctgctcgg ccgcctgcgc
     4921 ggcagcggag cgcgggtcga ggaaaccagc gacgagacgc tcgagtcgat ccaggacgcc
     4981 cgctcaccgc agcccgtcgt cgggctgctg cgctttccgg ccgacgccgg cgccgccgtc
     5041 cggtcggtgc tcgccgccgc cgagctcgtg ttggtcctcg attcgatcca ggaccccggc
     5101 aacctcggct cgatcgtacg ctcggcggat gccgcgggct gcgacgcgtg cttcgtcatc
     5161 ggtcgatcgg ccgacccgct ccacccgcgg gcggtgcgcg gggcgatggg ctccgtcggc
     5221 cggctgtggc cccgcccggc gccgtcggac gagttgttcg gcgcgctggc cgcgagcggc
     5281 catcgcgtcg tcggcagcga tccgcacgac ggagcgccgt atgacgcgga ggacctgacc
     5341 ggccgcatgg cgctggtgct cggcggggag gcggccggca tcagccgcac cgcgcgcggg
     5401 cggctgacgc ggacggtgcg catcccgatg cgcgccggga tcgacagtct gtcggtggcc
     5461 gccgcgggtg cggtgttgtt gttcgaggcc gcgcgtcagc gacgccggta gcggcggatc
     5521 acgcggaagc gcggccgggg cgcggcccgc gtcgatcgat ccgaggccga cgcctcgtcg
     5581 tcgcccgctg ccggcgtgcc cgcctccgcc gcgtcccggc ggtcgggctc ccgatcgcct
     5641 tcgggcgcgc gcggcagggt gaacgagacg cgggtccacg cgcccggctc gctctcggcg
     5701 cgcacgcggc agccgtgcag actgaggatc tcgcgcacga tcgccaaccc gagcccgcgg
     5761 ccggactccc cgcccccggt cgtcgcgacc cggtagtggc gatcgaagac gcggggcagg
     5821 tccgcggcgg ggatgccttt gcccgagtcg cgcacggcga cctcgaggta ccgcggttcg
     5881 cccggcccga ccgtgatctc gatcgatccg cccggcttgt tgaacttgat cgcgttcgac
     5941 agcaggttga tgaacacctg ctcgaccttc gcgcgatcgg cttggacccg tgcgccgtcg
     6001 tcgctcatcg acagttcgaa gtgaatgccc ttggcctgca tctcgcgacg cagcaggtcg
     6061 gcggtctgct cgatcagctc gcgcagcaga aaggtggtcg gcttcagctc cgccaagtcc
     6121 ggagcgtggg tcaagctgtc gatcaggccg atcagccggt cgatgttgcc caggctcagc
     6181 gtcagcccct tccgctgctc ggccgtgatc ggtcccaacc gctccttgag cgtcatctcg
     6241 gtgtaaccac ggatcgcgac cagcggagcc tgcagctcgt gcgacagatt gcccagcagt
     6301 ttctgccgca gctcgtcgag ccgctgcagc tcgcgattca acttgcccag atcctccgta
     6361 cgcgaggcga gatcgcggtt ggcgcgatcg agctcggcca acgcccgctc gagctcgacg
     6421 ttgctcaact gcagtttctc ggcgtgctgt tgtagctcgc gctcggcatc ccgctggcgg
     6481 gtcgtgtcgc ggcagacgac gacccggccg atgcgtttgc cgtcgcgatc gaccagggcg
     6541 aaggtcgcga ccgacaggtc cttgggcgcg gcgccgccca gacgcacggg ccgtccgttc
     6601 ggctcgaccg tgccggaggc cagcaactcg gcgacggccg ccgcaccgtc ctcgcgttcg
     6661 cgcaaggcgc ggatcagccg gccctcgggt tgcccgagca cctgcggctc acggagaccg
     6721 aagatgcgca ggaacgcgcg gttggtcatc tggacgacgc cgccgcgccc gcggtcgacc
     6781 agcacgagca tgccgtcgcg tgtcgcctcg agcacggcgt cgagctgcga ttcgttgcgg
     6841 cgcagctcag tctcgatccg tcgcgcggcg gtttcgtcgt ggaccagcag cagtacgccg
     6901 atcccgccct cgaactctac gctcgttgcg tggatccgta cgtcggcccg cggtcgatcg
     6961 tcggcgccgg ccagcgtgca gcgcatctcg tcggtcccgg cgccaccgag ttcgagcgcc
     7021 tcgagccgcg cgtcgatcat caatacgtcg cgcgtcacca cgcggtcgcg cagccgcgtg
     7081 ccgatcagcg tttcgatcgg cagcccggtc atcgcgctga acgcggggtt gacgtagacc
     7141 aacgtccctc ggctgacgat cgcgacgccg tcggtcaggc tttcgaccag ccggcgatga
     7201 cgctcctccg acaggcgcag ctcctgttcc agtccggcct gacgggtcac gtcgcgcaac
     7261 agcatgagga agccggtcga ttcgcccttc ttcgatcgga tcggacggac ggtgacttcg
     7321 gcggggaact gactgccgtt cttccgccga agcaggctgc gcgagatgac tccaccgtcg
     7381 aaggttgccc gccgcgagag ctgcggcacg aactcgcgga acgcgtcctc ctcgaagatc
     7441 atcgacgacg gtcggccgcg cgcctcgtcg gcggaccacc ccagcatctg ttccgcgctc
     7501 tcggtgaaat cgcggatgtc gccgtcgagg tcgatggtga gcaccgcgct gtcctggaac
     7561 gcctgcatga cgagctgcag gcgttcgcga tcggcggcgg cctcggccag ccgcagcttg
     7621 acgtcgtgcc ccgcccggtg gatcgcatcg gccaccacgc tcagcggcga gccggggtcg
     7681 aggtcgacac gcgcggcgta gctgcccgag cgcagctcct cgagcgcgtg gaccagccgg
     7741 gtgacgtcct cggtgcgcga gcggcgcgcc agcgaacgaa tcagcagcag gacggacagc
     7801 gccaacagca gcaccaggcc gagcaggccg ccgagcgcca gggcggttgc gttcgcccgt
     7861 tcgaacaggc cggcggccag cgcgacaggt ggttgaacca tcgtccatcc cgacggacga
     7921 ggcccggcgg accggacctc cgggagacgt ccggtcgggc tagggcacgc gcccgcgcaa
     7981 ctcttcgatc cgcgacgtcg gtcgggcgaa cagatgccct tgaccgtatc gcgcccccgc
     8041 ctcggtcagc gcccgagcct cttgctcggt ctcgacgccc tcggcgatga cggaggcccc
     8101 gatacgagtc gctatacgca ctaaggaacg caaaagctct tgctttatca ggttctggtc
     8161 gattttccgc accagcgtga cgtccagttt caggaagtcc gggcgcagtt cctcgaggat
     8221 cgcctggctg gtgaatccgg tgccgatgtc ctcgaccgag acggcgagac cgtggcgttt
     8281 caaccggtcg agcgtcgaaa cgaaaccctc cgggtcgccg tcggcggcgc gctccgagaa
     8341 ctcgagcacc catcgctccg gctcgtcacg gagcggccgc agtcgctcgg gccaagcggc
     8401 cgcggcggtc ggaacggcgc ggaaactgtc gggcaggccg ttgaggaaga ccttgcccgg
     8461 ccgggggagg cgcgccgcgg cctcgaccgc cagcttccgg cagagcgagt cgaggtcgcg
     8521 cgagaccccg agccggtccg acacgcggaa cagcatccgg ggcgcctcga gcgcggtgtc
     8581 gaccggaccg cgggcgaacg cctcgtagcc gagggtcgcg ccggatcgta gatcgacgac
     8641 cggctggaag acggtacgca gctcggcgtc acgcaggatg cggagcaggt cgtcgccgtg
     8701 accgcgcagc tgtcgaccca agcgccggtg gtcggccgag cgcgcctggt cgatcgcggt
     8761 atacagctgg cgctcgaacc gaaagaacgg gtcgtggctg agcatcgcgt gacccaggcg
     8821 gaactcgagc ttggggttga gtccggcgaa gtcgtcgccg gcgaatgccc ggtcgagccg
     8881 tctgcgcagc gtctccgccg tctcggccag gtagcgggta tcgacgtcac cgccgtcgag
     8941 gcgggaggcg acgaacagcg cgaagcgatc gccggcgacg ccgttcaggc ccaacgccgc
     9001 gtcggcgccg cacagctcgc gcacgccgct cgtcagcacg ccggcgaccc gctccaggat
     9061 gcggtcgaac acctgccagc cgtagagcga ctcgacgagg ctcaggttct ccgcctcgac
     9121 gtggatcacg ccgatcgccc ggcgcagctc gagccagccc cgcagacggt cgatcagcac
     9181 cgggaaggaa ggcagccccg tgatccggtc gtagagcacg ctgcgcagct tcagatagtg
     9241 ccagcgctcg ggttccgtcc gctctccgtt cgggctcacc tcacgccacc ggctggctgg
     9301 tcggcgggcg cggcccgccg cgccgcgcct cgagtacccg ccggacgcga tagagcagct
     9361 gatcgacctc gaacggcttg gtgatgtagt ccaccgcgcc ttcctgcatg ccgagcaccc
     9421 gatcgtggat tcggtccttg accgagaaca tgacgaccgg caggtcggcc agcgagtcgt
     9481 cggcgcgaat cagccgcagc gtctcccacc cgtccatctc cggcatgttg atgtcgagca
     9541 gcaccagctg gcacgagtgc gcgaccagat ggcgcaacgc gtcggcgccc gacggcttgg
     9601 caacgacgtc gtaaccggcg ccctcgagca gcagggccgt cagctcgcgg atatcggcct
     9661 gatcgtcgac gaccatgatc gagggtcgcg gttgcatcgg acgcccattc tatgtcagcg
     9721 tccgctacgg tgcgaggccg tcctgccgga agcgggttga acccggcgcc gggtgatccg
     9781 tacaaaggtg gagcaccata acgatttggg ggtgggaaga tgagcaagat tccgttctcg
     9841 atcgcactgc tcgcgctggc cgccgtggcc tttgccgggg cggcggcgga gaaggcgaca
     9901 gacgactgga cccagtgggg cggaccggat cacgagttcc gcgccacgag cgccggcttg
     9961 gccaagcagt ggtcggccga cggaccgcgc gagctgtgga cgcgagagtt gggcgacggg
    10021 tactcggcga tcctctatca ggacgggaag ctgtatacga tgtatcgcag cggagacgag
    10081 gaagcggtcg tctgcttgaa cgccaagacc ggcaagaccg tgtgggagca ccgttaccgg
    10141 gcggcccccg ccgaaggcca cgtcagccag ttcggcgaag gcccgcgttc gacaccgctg
    10201 atcgccggcg ggatgatcta cacgatcggt gtcgcgggag acctgtacgc gctcgacaag
    10261 tcgaacggca agccggtgtg gaaccacgac ctg
//
LOCUS       DASPGN010000045         7318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_9626, whole genome shotgun sequence.
ACCESSION   DASPGN010000045 DASPGN010000000
VERSION     DASPGN010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7318
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9626"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VD788_01395"
     CDS             <1..516
                     /locus_tag="VD788_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74945.1"
                     /translation="LVEVTIEGLGTIREGFDGEVGWSTDPMSGPQLKRGQHLLQTKEQ
                     ALYTSVLFPADQYKMREVVERVEFDGRPAWKIRFVSTNDNESFGYFDVDSGLQLGAEL
                     TQRTPMGDIPVRTSFRDYREFDGVMIPTRIEQDLGPAGTQIIEVAKVTSGGVDASRFA
                     LPAEIKALTEQ"
     gene            complement(563..1663)
                     /locus_tag="VD788_01400"
     CDS             complement(563..1663)
                     /locus_tag="VD788_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV74946.1"
                     /translation="MAMTEKQGGSDVRASSTVAVPLSAAGEYELTGHKWFCSAPMSDA
                     FLTLARTAAGLSCFLVPRWRPDGTRNPFLIQRLKDKLGNRSNASAEVEYHATWAARVG
                     EDGRGVRTIIEMVHQTRLDAATAPVGMMRWALAEARHHAEHRRAFGARLIEQPLMRNV
                     LADLALEREAGLALVMRVAQAFDRAATDGAERSFARLATAAAKYLTNKRAAAFVCEAM
                     ESVGGGGYVEESLLPRLYREAPLNGIWEGSGNVICLDVLRTIEREPQAVATLLAELDL
                     VAGHHPVFDRALAGLRRQLADPHEAEWRARRLTERIALLLEAALLVRHAPSEVAEAFC
                     ATRLDGGAGPRVYGTLPAGTAVDRLLDRALPA"
     gene            complement(1721..2221)
                     /locus_tag="VD788_01405"
                     /pseudo
     CDS             complement(1721..2221)
                     /locus_tag="VD788_01405"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2244..5609)
                     /locus_tag="VD788_01410"
     CDS             complement(2244..5609)
                     /locus_tag="VD788_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV74947.1"
                     /translation="MSLPDFSVRRPVTILVTTVALLIFGYLSWSNIPIELLPDLSYPT
                     LTVQTEYADAAPTSVEQFITRPLEEAVGVISGVREMRSVSRAGLSEIVLEFEWDERME
                     FAAMEVREKLGLVELPREAERPRVLRFDPTLDPIVRLAFTGDRPPVDLRQIADRWLKP
                     RLEAIQGVAAAKVRGGLEPEIQVQADEDRLAALGLTLDDLAQALRAENVNRPGGRLKD
                     WGAVYLVRTLHEFTDLDQLRRTVVRESPAGRVRVEDVAQVFRGHRDPQEITYNGGART
                     VEIALHRDGSSNTIAVSRAIRQALSGPTGLRAQLSDDLELTYLSDQSIYIVEAIGQVW
                     SAALIGGLLAILVLYFFLRDVRATVIIGLSIPISVVVTFLPMYKSGVTLNIMSLGGLA
                     LGVGMLVDNSIVVLEAIDRKRREGSVGKDAATEGARNVVGAVTAATLTTVSVFLPIVF
                     VRGVAGQLFYDLAVTVCLALVMSLFVSLTLIPMLSAFELGRVRRTSLEQAFGATSGDS
                     PWRTIGQLLRIPRARPAAGRLGRALRGSAVALRFLLRLVVSLPLYLIAVIVRGLARLV
                     VAAIVWSWSVVSRLIDWLFLPATWLFDGIGRAYPRGLAGALRARRGVLAAGFAAFVLA
                     AAAVPLLGTNLVPDLSQGEFAFRIRLPEGTPLEASAETIERIERRCLDDGRFERVFSV
                     VGNWPSTASGRQTVGENLAQINFVLPDGTPAQDEAAAIEEVRRVLAVFPRVEAELAHP
                     SVLSVRPPLAVNLFSENLDDLDLAAVRVAEMIWTIAAVEDVATSSEPGNPEIRIELDR
                     ERAADLDVQAEPLSRSLQRQIGGEIVGQFRELEQRLDIRLRALEAWRDTAGEVEDLRY
                     RLENGTSVPVSAFASVTLERGPAAIYRNGGARVATVTAKLDSTDLGRSMDELKTRLAG
                     LELPESVVAESAGQDKELQVSFRSLRLALALAVFMVYVVMAVQFESLRHPFVILLSVP
                     LGIIGVVLGLFLTDTSVSVLALIGAVMLAGIVVNNAIVLVDAINLLRREQGMAPYDAV
                     IEAGRLRLRPILMTTLTTVLGLTPLALGIGAGAEIQAALARTVVGGLLASTLVTLVLI
                     PVTYVTTTGLFETVRSTERAREPVPGGSPASVG"
     gene            complement(5596..6789)
                     /locus_tag="VD788_01415"
     CDS             complement(5596..6789)
                     /locus_tag="VD788_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV74948.1"
                     /translation="MRIPPQRERRTRPARAAVAAVSLGVVVGAGLIVGATGCGGANEA
                     QSRPGASAASHSEAAEEKVADDAIAVAVAKVARAPLSSLYSTSATLRADKRATVTART
                     RGVVEQLLVEEGDWVAAEQPVAVLENDEQRIAFEQAAAISATKDREYARAESLHAQGL
                     LSEEEYETTRREAVELRHAAELAELNLSRTVIRATFAGQVLTRHLDPGATVADGTPVY
                     DIADLDPLYADIQVPERQIEQLLPGQTVRLLNETDQPVAEASIERIAPAVDPTTGTVK
                     VTVSVSYDRELRPGSFVRVGIVTDTHEHALVVPRSALVSEGRRWHLFRIKDGNDSAVE
                     LIEITRGFEESDRVEVLGVAGEVRPLREGDRVVVRGASSLSADSVVQIIEETGDDSGA
                     SGVAA"
     gene            7002..>7318
                     /locus_tag="VD788_01420"
     CDS             7002..>7318
                     /locus_tag="VD788_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74949.1"
                     /translation="MRASNRGIAGLAGAALFLIAHAATPAQDEEPPKLLGRCTTSQLD
                     QEPYAEWFRAGYADYRPNAEVLASLRRLDLDRLGIDVFFGTWCGDSRRELPRLLKLLD
                     ELD"
BASE COUNT         1189 a         2614 c         2477 g         1038 t
ORIGIN      
        1 ctggtcgagg tcacgatcga ggggctcggc acgattcgcg agggcttcga cggcgaggtc
       61 ggctggtcga ccgacccgat gagcggcccg cagctcaagc gcggacagca cctgctgcaa
      121 acgaaagagc aggcgctcta cacgagcgtg ctgttcccgg ccgaccagta caagatgcgc
      181 gaggtcgtcg agcgggtcga gttcgacggc cggccggcgt ggaagatccg tttcgtctcg
      241 accaacgaca acgagtcgtt cggctacttc gacgtcgaca gcggcctgca gctcggagcc
      301 gagctgaccc agcgcacgcc gatgggcgat atcccggtcc ggaccagctt ccgcgactac
      361 cgggagttcg acggtgtgat gatcccgacg cggatcgagc aggacctcgg tccagccgga
      421 acgcagatca tcgaggtcgc caaggtcacg agcggaggtg tcgacgcctc gcgcttcgcc
      481 ctgccggccg aaatcaaggc gctgaccgag cagtgacgag ctcgcggctc gcttcgcgtc
      541 ggtccggccg gcggcgtccg tctcacgccg ggagggcgcg atcgagcagg cggtcgacgg
      601 ccgtcccggc cggcagcgtg ccgtagaccc gcgggccggc gccaccgtcg agccgggtgg
      661 cgcagaacgc ctcggccacc tcggacggcg cgtggcgcac gagcagagcg gcctcgagca
      721 gcagcgcgat ccgctcggtc agccggcgtg cgcgccattc ggcctcgtgc gggtctgcga
      781 gctggcggcg gagcccggcc aacgcgcggt cgaagaccgg gtggtggccg gccacgaggt
      841 cgagctcggc cagcagcgtc gcgaccgcct gcggctcgcg ctcgatcgtg cgcagcacgt
      901 cgaggcagat cacgttgccc gagccctccc agatgccgtt gagcggggcc tcgcggtaca
      961 gccgcggcag cagcgattcc tcgacatagc cgccgccgcc gaccgactcc atcgcctcgc
     1021 agacgaacgc cgccgcgcgt ttgttcgtca ggtacttggc cgccgccgtc gcgagccgcg
     1081 cgaacgaacg ctcggccccg tcggtcgccg cgcgatcgaa ggcctgcgcc acacgcatca
     1141 ccagcgccag cccggcctcg cgctcgagcg ccaggtcggc cagtacgttc cgcatcaacg
     1201 gctgttcgat caaccgggcg ccgaacgcgc ggcggtgctc cgcgtgatga cgcgcttcgg
     1261 ccagcgccca gcgcatcatt ccgaccggcg ccgtcgccgc gtcgagccgg gtctggtgca
     1321 ccatctcgat gatcgtgcgc acgccgcgtc cgtcctcgcc gacgcgggcg gcccaggtgg
     1381 cgtggtactc gacctcggcc gacgcgttcg accggttgcc gagcttgtcc ttcagccgct
     1441 ggatcaggaa cggattgcgt gtgccgtccg ggcgccagcg cggcacgagg aaacagctga
     1501 gccccgccgc ggtgcgggcc agcgtgagga aagcgtcgga catcggcgcc gagcagaacc
     1561 acttgtggcc ggtcagctcg tactccccag cggccgacaa cggcacggcg accgtgctgc
     1621 tcgcgcggac gtccgagccg ccttgcttct cggtcattgc catgccgaag gtgacacccc
     1681 gtttctctcg cgccggcagg cagcgcgcgt cgtagaccgg tgccgacagc tttggttccc
     1741 actgctcggc cagctccggc tgatgacgca gcgcgggcac gaccgcatgg gtcatcgtga
     1801 tcgggcagca gacgccctac tcgggctgcg tcagcatgta gatcgccgcg gcgtgggcca
     1861 ccgcgccgcc gtcgccggcg tgccgccagg cgatcgagtg cacgccatgc tcgatcgcca
     1921 tcgccatcag ctgatggtac gccgggtggt aggacacctc gtcgattcgc cggccgcgtc
     1981 ggtcgaacgt gaccagacgc ggcggatggc ggttggcctc gaaacccagt tggcgagcgt
     2041 gctcgctgcc gagccgcggg ccgagcgccg cggcccacga cccgacccac gccggcgcgt
     2101 gccgcgtcac cgtctcgacc agcgcggcat cgccggccca caggtcgagg tcgccgatct
     2161 ccggaggctg gttggtcacg tcgtgggtcg gcagccggct gacggggtcg cgcggaggca
     2221 tgacgcagag caggttacca gggtcagccg accgatgccg gtgagccgcc gggcaccggt
     2281 tcgcgcgccc gctcggtcga gcgcaccgtc tcgaacaacc cggtcgtcgt cacgtaggtc
     2341 accgggatca gcaccagcgt gaccagcgtc gaggccagca gaccgccgac caccgtccgc
     2401 gccagcgcgg cctggatctc ggcaccggcg ccgatgccga gcgccagcgg cgtcagtccg
     2461 agcaccgtcg tcagcgtcgt catcaggatc gggcgcagcc gcagccgccc ggcttcgatc
     2521 accgcgtcat acggcgccat cccttgctcg cgccgcagca ggttgatcgc gtcgaccagc
     2581 acgatcgcgt tgttgaccac gatgccggcg agcatcaccg cgccgatcag cgccagcacg
     2641 ctgacgctcg tgtcggtcag gaacagaccg aggacgacac cgatgatccc gagcggcacc
     2701 gagagcagga tcacgaacgg gtggcgtaac gactcgaact ggaccgccat cacgacgtag
     2761 accatgaaaa cggcgagcgc cagcgccaag cgcaggctgc ggaacgagac ctgcagctct
     2821 ttgtcctgtc cggccgattc cgcgacgacg ctctccggca gctcgagccc ggccaagcgg
     2881 gtcttcagct cgtccatcga gcgcccgagg tccgtcgaat cgagcttggc cgtgaccgtc
     2941 gccacgcgcg cgccgccgtt gcggtaaatc gccgccggtc cgcgctcgag cgtgaccgac
     3001 gcgaatgccg agaccgggac gctcgttccg ttctccaacc gatagcgcag atcctcgacc
     3061 tctcccgcgg tgtcgcgcca ggcttcgagc gcccgcagac ggatgtcgag ccgttgttca
     3121 agctcgcgaa actgaccgac gatctcaccg ccgatctgcc gttgcagcga gcgtgacagc
     3181 ggttccgcct gcacgtcgag gtcggccgca cgctcgcgat cgagctcgat ccggatctcc
     3241 gggttgcccg gctcggacga ggtcgcgacg tcctcgacgg ccgcgatggt ccagatcatc
     3301 tcggccaccc gtaccgcggc gagatcgaga tcgtcgaggt tctccgagaa caggttgacc
     3361 gccagcggcg ggcgcaccga cagcaccgac ggatgcgcca gctcggcctc gacgcggggg
     3421 aagaccgcca gcacccggcg cacttcctcg atcgccgcgg cctcgtcttg cgccggtgtc
     3481 ccgtcgggca ggacgaagtt gatctgggcc agattctcac cgacggtctg gcgaccggag
     3541 gccgtcgacg gccagttgcc gacgacggag aagacacgct cgaaccggcc gtcgtcgaga
     3601 cagcgccgct cgatgcgttc gatcgtctcc gcactggcct cgagcggagt gccttcgggc
     3661 aagcggatgc ggaaggcgaa ctcgccttgc gacaggtcgg gtaccagatt ggtgccgagc
     3721 agcggcacgg cggccgccgc caggacgaat gcggcgaagc cggcggccag cacgccgcgg
     3781 cgcgcccgca gcgcaccggc caacccccgc gggtaggccc gtccgatgcc gtcgaacagc
     3841 cacgtcgccg gcaggaacag ccaatcgatc agacgcgaca cgacggacca cgaccagacg
     3901 atggccgcga ccacgagccg ggccagcccc cgcacgatca ccgcgatcag gtacaacggc
     3961 agcgaaacga cgaggcgcag caggaaccgg agcgccaccg ccgatcctct cagcgcgcgc
     4021 cccagccgac ccgctgccgg cctcgctcgc gggatccgca gcagctggcc gatcgtgcgc
     4081 cacggcgagt cgccgctcgt cgcgccgaac gcctgctcga gcgacgtgcg tcgcactcga
     4141 cccagctcga acgccgacag catcggaatc aaggtcaacg agacgaagag cgacatcacc
     4201 agcgcgagac agacggtcac cgccaggtcg tagaacagct gccccgccac gccgcggacg
     4261 aagacgatcg gcaagaagac cgaaacggtg gtcagcgtcg ccgcggtcac cgcgccaacg
     4321 acgttccgcg cgccttccgt cgccgcgtcc ttgccgaccg acccttcgcg ccgtttgcga
     4381 tcgatcgcct cgagcacgac gatcgagttg tcgacgagca tgccgacgcc gagcgccaag
     4441 ccgccgagcg acatgatgtt cagcgtgacc ccgctcttgt acatcggcag gaacgtgacg
     4501 acgaccgaga tcgggatcga caggccgatg atgacggtcg cgcgaacgtc gcgcaggaag
     4561 aaatagagca cgaggatcgc gagcaggccg ccgatcagcg ccgcgctcca cacctggccg
     4621 atcgcctcga cgatgtagat cgactggtcg ctgaggtagg tcagctcgag gtcgtcgctg
     4681 agctgcgcgc gcagcccggt aggacccgac aatgcctgcc gaatcgcacg ggagactgcg
     4741 atcgtgttgg acgagccatc gcggtgcagg gcgatctcga cggtccgagc gccaccgttg
     4801 taggtgatct cctgcggatc gcggtgccca cggaacacct gcgccacgtc ctcgacgcgg
     4861 acgcggcccg ccggcgactc gcgcacgacc gtgcgtcgta gttgatcgag atcggtgaac
     4921 tcgtgcagcg tgcggacgag gtagaccgct ccccaatcct tcagccgccc tccgggacga
     4981 ttgacgttct cggcacgcaa cgcctgcgcc aggtcgtcga gcgtcaaacc gagtgccgcc
     5041 aaccggtcct cgtcggcctg cacctggatc tccggctcga gcccgccccg caccttggcc
     5101 gcggcgacgc cctggatcgc ctcgagccgc ggcttgagcc agcgatcggc gatctgacgc
     5161 aagtcgaccg gcgggcggtc gccggtaaac gccagacgga cgatcgggtc gagggtcgga
     5221 tcgaaacgaa ggacgcgcgg ccgttccgcc tcgcgcggca attcgaccag ccccagcttc
     5281 tcccgcacct ccatcgcggc gaattccatc cgctcgtccc attcgaactc gagcacgatc
     5341 tcggacagac cggcgcggga gaccgaacgc atctcgcgta cgcccgagat caccccgacc
     5401 gcttcctcca gcgggcgggt gatgaactgc tcgaccgagg tcggtgcggc gtcggcgtat
     5461 tccgtctgga ccgtgagcgt cgggtaggac aggtccggca gcagttcgat cgggatgttg
     5521 gaccacgaca ggtagccgaa gatcaacagc gcaaccgtcg tcaccaggat cgtgaccggg
     5581 cgtcggaccg agaaatcagg cagcgacacc gctcgctccg gagtcgtcgc cggtttcctc
     5641 gatgatctgc acgaccgaat cggcgctcag cgacgacgcg ccgcgaacga caacgcggtc
     5701 gccctcccgc agcggcctga cctcgcccgc gacgccgagg acctcgacgc gatcgctttc
     5761 ctcgaagcca cgggtgatct cgatcagctc gaccgccgag tcgttgccgt ccttgatgcg
     5821 aaagaggtgc cagcgccggc cctcggagac cagcgccgag cggggaacga ccagtgcgtg
     5881 ctcgtgggtg tcggtgacga tgccgacgcg gacgaagctg ccgggtcgca actcgcggtc
     5941 gtagctcacc gagacggtga ccttgaccgt gccggtcgtc ggatcgaccg cgggcgcgat
     6001 acgctcgatg gaagcctccg cgacgggttg gtcggtctcg ttgagcaggc ggacggtctg
     6061 tccgggcagc aactgttcga tctgacgctc gggtacctgg atgtcggcgt acagcgggtc
     6121 gagatcggcg atgtcgtaga ccggcgttcc gtcggcgacc gtggcgcccg ggtcgaggtg
     6181 tcgtgtcagt acctgtccgg cgaaggtcgc ccgaatcacc gtgcgcgaca agttgagctc
     6241 ggcgagctcg gcggcgtgcc gcaactcgac cgcctcgcgt cgcgtcgttt cgtactcttc
     6301 ctcgctcaac agcccctgcg cgtgcagcga ttcggcgcgg gcgtactcac gatccttggt
     6361 cgccgagatc gccgcggcct gctcgaacgc gatgcgctgc tcgtcgttct cgaggaccgc
     6421 cacgggctgc tcggcggcga cccagtcacc ctcttcgacg agcagttgct cgaccacgcc
     6481 ccgcgttcgc gcggtcaccg tcgcgcgctt gtcggcgcgc agggtcgcgc tggtggaata
     6541 cagcgatgag agcggtgccc gcgcgacctt ggcgaccgcg acggcgatcg cgtcgtccgc
     6601 gaccttctct tcggccgcct cgctgtgcga cgcggccgaa gcccccgggc gcgactgggc
     6661 ctcgttggcg ccgccgcaac cggtcgcacc gacgatcaac cccgctccga cgacgacgcc
     6721 cagggagacc gccgcgactg ccgcgcgagc gggtcgcgtg cgccgttccc gctgcggtgg
     6781 gatgcgcatc ggtgtccctc ccctccagac ctcgctgaac taaacggccc gggtcgacga
     6841 aaggtttcgc ggaagccagg aatcggctac caaggtactt ctgcgcgacc gatccgtcga
     6901 ccccctcgcg ccggccgctt tttacctacg cgttacaacc cgaggggtcg cgcgacgtgt
     6961 aagacttccc catcggcgcg agcccgccga cggaggttac gatgcgagcg agcaaccgtg
     7021 gaatcgccgg cctggccggc gccgcgctgt tcctgatcgc ccatgccgcg acgccggcgc
     7081 aggacgagga gccgccaaag ctgctcggac gatgcaccac gagccaactc gaccaggaac
     7141 cgtatgccga gtggtttcgc gcgggctacg cggactaccg gccgaacgcg gaggtgctcg
     7201 cgtcgctcag acggctcgat ctcgatcgcc tcggaatcga cgtcttcttc gggacgtggt
     7261 gcggggacag ccggcgtgag ctgccgcggc tgctcaagct gctcgacgag ctcgactt
//
LOCUS       DASPGN010000046         3010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_712487, whole genome shotgun sequence.
ACCESSION   DASPGN010000046 DASPGN010000000
VERSION     DASPGN010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712487"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VD788_01425"
     CDS             <1..792
                     /locus_tag="VD788_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74950.1"
                     /translation="WGEAGTLRLPLGQPVELLGRSFTYTGHVDGSEPQDRWRIEVARG
                     AQRPVAAEVAMYRTAGGRDSQIMHKPAILRGLSGDLYLAPAGLESLAEHQQVELVKGE
                     PLPVGPARLTFLQFETHGMGGGGMTVVARVLLEQEGRSETLRLPFTMAGGRTETPAVE
                     PTIESAFASLQLASMAVEQQRILVHVDVPADRATQTLALNVSTKPMIGLLWAGTILLG
                     CGCLLAWTRRLVDERAIARAVADSARAEVVAKAGPRRAPRPAHAR"
     gene            853..1539
                     /locus_tag="VD788_01430"
     CDS             853..1539
                     /locus_tag="VD788_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV74951.1"
                     /translation="MTDRTLLDFDGVWKSYSRPGGRQLHALREVTVAAGAVDSIAIVG
                     RSGSGKSTFLHLAAAIDEPTRGRIRFAGRDIGAMSERERTLHRRENIGLVFQFFHLLP
                     HLNVRDNVALPAIIAGEPRARYVERAELLLARVGLADRVTDDVLRLSGGEMQRVAICR
                     ALLRRPRLLLADEPTGNLDDDTGRSVMDLLLALVREEQSALIYVTHSRETAARADRTW
                     RMHSGTLDPS"
     gene            1536..>3010
                     /locus_tag="VD788_01435"
     CDS             1536..>3010
                     /locus_tag="VD788_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV74952.1"
                     /translation="MIGYFVRSLGAHFRSGRTLFLLSVLGVALGVASVLSIQLINLNA
                     LGAFEASAEAISGDADLSILPSTAAMSEAVYLDVLGVAGVEAAWPLVRLDVALRGVED
                     YHLELLGVDLFAPMRLPWEVAPQAAGDPLTTPGWVAVSPGLAGRFGWSLGDRFEVSSG
                     STVVELVVGALVDFREISPAASSRMVVIDIAQAQHLFGMRGVVQQIDLRLRDGAVADE
                     VRERLAVRVDPAAHVRTPEQRRLQTAELLGAFRLNLTALSLISLFVGAFLVYSATRSS
                     LARRHAEFGLLRSIGATGGQTFGLILAEVGLLGLLGVLLGLPLGYWVARRNVEIVSAT
                     LSNIYLLREIESLVVPFRWFALAAAIGIGGALVGAVGPATDFSRRDPRVLLAAYSLRD
                     SAIRWSRPLFVAGCATMLLGYLTHRLVPADWRPAGFLVALALVFGIPMLAPWTIERVT
                     AAMHSSRFGLLHGVKALGLELQSSAFAVAALAVAVTMLVGVT"
BASE COUNT          378 a         1044 c         1086 g          502 t
ORIGIN      
        1 tggggcgagg ccggcacgct gcggctgccg ctcgggcaac cggtcgaact gctcgggcgg
       61 agcttcacct acaccggtca cgtcgacggc tccgagcccc aggaccgctg gcgcatcgag
      121 gtggcgcgcg gcgcccagcg accggtggcg gccgaggtcg cgatgtaccg caccgccggt
      181 ggccgcgaca gccagatcat gcacaagccg gcgatcttgc gcggtctgtc gggcgatctc
      241 tatctggcgc cggccggact cgagtcgctc gccgagcatc agcaggtcga gctggtcaag
      301 ggggagccgc tgccggtcgg tccggcgcgg ctgaccttcc tgcagttcga gacgcacggg
      361 atgggcgggg gcggcatgac cgtcgtggcc cgcgtgctgc tcgagcagga aggtcgtagc
      421 gagaccctgc ggctgccgtt cacgatggcc ggcggtcgga ccgagacgcc cgccgtcgaa
      481 ccgacgatcg agtcggcctt cgccagcctg cagctggcga gtatggcggt cgagcagcaa
      541 cggatcctgg tgcacgtcga cgtcccggcc gaccgtgcaa cccagacgct ggcgctgaac
      601 gtcagcacca agccgatgat cgggctgctc tgggccggca cgatcctgct gggttgcggc
      661 tgtcttttgg catggacacg gcggttggtc gacgagcgcg cgatcgcgcg cgccgtcgcc
      721 gacagcgcgc gcgccgaggt cgtcgccaag gccgggccgc gccgagcgcc gcgaccggct
      781 cacgcccgct gacgccgttg gcggagtccg ttgccgcccg ataccttaag gggtccggcg
      841 cgagacggac ccatgacgga tcggactctg ctcgacttcg acggagtgtg gaagagctac
      901 tcccgacccg ggggccgcca gcttcatgcg ctgcgcgagg tgacggtcgc cgccggcgcc
      961 gtcgacagca tcgcgatcgt cgggcgcagc ggtagcggca aatccacctt ccttcacctg
     1021 gcggcggcga tcgacgagcc gacgcgcggc aggatccgtt tcgccggacg cgacatcggc
     1081 gcgatgagcg agcgcgaacg gacgctgcac cggcgcgaga acatcggtct cgtctttcag
     1141 ttctttcatc tgctgcccca tctgaacgtg cgtgacaacg tcgccttgcc ggcgatcatc
     1201 gccggcgagc cccgcgcccg atacgtcgaa cgtgcggagc tgctgctggc ccgcgtcggc
     1261 ctggccgacc gcgtcacgga cgacgtcctg cggctgagcg gcggcgagat gcagcgcgtc
     1321 gcgatctgcc gcgcgctgct caggcggccg cggctgttgc tggccgacga gccgaccggc
     1381 aacctggacg acgacaccgg ccgcagcgtg atggacctgc tgctcgcttt ggtgcgcgaa
     1441 gagcaaagtg cgctgatcta cgtcacccac agtcgcgaga cggccgcgcg cgccgaccgc
     1501 acctggcgca tgcacagcgg cacgctggac ccgagttgat cggctacttc gtccggtcgc
     1561 tcggcgccca ctttcgttcg gggcgcacgc tgttcctgct cagcgtgctg ggtgtcgcgc
     1621 tcggcgtggc ctcggtgttg tcgatccagc tgatcaacct caacgcgctc ggcgcgttcg
     1681 aggcgagcgc cgaggcgatc agcggcgacg cggacctgtc gattcttccg tcgaccgcgg
     1741 cgatgtccga agcggtctac ctcgacgtgc tcggcgtcgc cggcgtcgag gcggcctggc
     1801 cgctggtacg gctcgacgtc gcgctgcgcg gcgtcgagga ttatcacctc gagctgctcg
     1861 gcgtcgatct gttcgcgccg atgcggctgc cgtgggaggt cgccccgcag gccgcgggtg
     1921 atccgctgac gacaccggga tgggtcgccg tctcgccggg gctagccggc cgtttcggct
     1981 ggtcgctcgg cgatcgcttc gaggtgtcga gcggatcgac ggtcgtcgag ctggtcgtcg
     2041 gcgcgctggt cgatttccgc gagatcagtc cggcggccag cagccggatg gtggtgatcg
     2101 acatcgcgca ggcacagcac ctgttcggca tgcgcggcgt cgttcagcag atcgaccttc
     2161 gcctgcgcga cggcgcggtc gccgacgagg ttcgcgagcg cctcgccgta cgcgtcgacc
     2221 cggcggcgca cgtccgcaca cccgagcaac gccggctgca gacggcggag ctactcggcg
     2281 cgttccggct gaacctgacc gcgctgagcc tgatcagcct gttcgtcggc gcgtttctcg
     2341 tctactcggc gacgcgctcg tcgctcgctc gccgacacgc cgagttcggt ctgctgcgat
     2401 cgatcggtgc gacgggcggt cagaccttcg gcctgatctt ggccgaggtc ggcctgctcg
     2461 gcctgctcgg cgtcttgctc gggctgccgc tcggctactg ggtcgcccgg cgcaacgtcg
     2521 agatcgtcag cgccacgctg agcaacatct acctgctgcg ggagatcgag tcgctggtcg
     2581 taccgttccg ctggttcgcg ttggcggcgg cgatcggcat cggcggggca ttggtcggcg
     2641 cggtcgggcc ggcgacggac ttcagtcggc gcgacccgcg cgtgctgctc gctgcgtact
     2701 cgctgcgcga ttcggcgatc cgctggtcgc gtccgctgtt cgtcgccggc tgcgcgacga
     2761 tgctgctcgg ctacctgacc caccggctgg ttccggccga ctggcgcccg gccggctttc
     2821 tcgtcgcgct cgctctcgtc ttcggtatcc cgatgctcgc gccgtggacg atcgaacggg
     2881 tgacggcggc gatgcacagc tcgcgcttcg gcctgctgca cggggtcaaa gcgctcggtc
     2941 tcgagctgca gtcgtcggcg ttcgccgtcg cggcgctcgc cgtcgcggtg acgatgctcg
     3001 tcggcgtcac
//
LOCUS       DASPGN010000047         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_9879, whole genome shotgun sequence.
ACCESSION   DASPGN010000047 DASPGN010000000
VERSION     DASPGN010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9879"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2324)
                     /locus_tag="VD788_01440"
     CDS             complement(<1..>2324)
                     /locus_tag="VD788_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV74953.1"
                     /translation="LGAIRGPELRYDVRSEARFDQIETIRDLDIGRRGLRLRDVARVE
                     LREPLLDYGRHLNRDFAIGFDVIKEASANTVETVDQLMAKIDEIGRDPELGGINLVVW
                     QNQGEEIRNSLRGLRNAGIFGGLLAVGVLYFFLRRLKTTLIVAVAIPFSLLVTCGARF
                     MFGMEFNVLTMLGLMLGVGMLVDNAVVVIENIYRLQAQGMSPDRAARLGARQVALAVL
                     AATATTIIVWSWLFVLEPNEMKIYIGAVAAVICISVVCSLIISMTFIPLAAARYVSDE
                     PIRRGFFVGRLVPRYRAILGWTLQHRALTLIALFGLAASAAIPIGLIEKTGQPKMQQR
                     EVLIVYQVADASSLEVLEGYVNRVEDWLEANKQELGYSDIYSWYTEGQNNCNTRVYLP
                     EGKNTETAFKRLEDKLRRGLPTVAGVEFEFGEHGGRHRGPRRGGMVSVAVHGEDPEYL
                     QELAYDVEGRLRGIEHVDEIYGPSLNGREEVQVLIDAERARALGIEPETIADTVGFAF
                     RGQHLRRFQGRSNEIEVIIGLPETIRPGLAAITDLPIPQPGGEFVSLGAVAEIDVTRT
                     PPFLNRVDRKTTSWVTLQFDEEQTTTSEIRSEVETRLAGLRLPEGYAWDWGQEMHDDD
                     EALAIMLRGIVISIAVVLLLMAALFESLSQPLAILITLPLALFGAFWTLWLAGFVFEI
                     LGFIGVIILIGVVVNNGIVMVDHVNGLRRSGTERGAALIEGCGDRLRPVLMTVITTVV
                     GLIPLAVSEFTVAGVYIQSMAVAMIGGLISSTVF"
BASE COUNT          412 a          787 c          742 g          383 t
ORIGIN      
        1 tgaaaacggt cgacgagatc aaaccgccga tcatcgccac cgccatcgac tggatgtaga
       61 cgccggccac cgtgaactcg gataccgcca gcgggatcag accgaccacg gtcgtgatca
      121 ccgtcatcag caccggccgc aggcgatcgc cgcagccctc gatcagggcg gccccgcgct
      181 cggtgccgga gcggcgcagg ccgttcacgt gatcgaccat cacgatgccg ttgttgacga
      241 cgacaccgat cagaatgatc acgccgatga agccgaggat ctcgaagacg aagccggcga
      301 gccacagcgt ccagaacgcg ccgaacaggg cgagcggcag cgtgatcagg atcgcgagcg
      361 gctgactcaa cgactcgaac aacgcggcca tcagcagcag caccacggcg atcgagatga
      421 cgatgccgcg aagcatgatc gccagcgcct cgtcgtcgtc gtgcatctcc tggccccagt
      481 cccaggcata gccctcgggc aggcgtaggc ccgcgagacg cgtctcgacc tcgctccgga
      541 tctcgctcgt cgtggtctgt tcctcgtcga actgcagggt gacccacgag gtggtcttgc
      601 ggtcgacgcg attgagaaac ggcggcgttc gtgtcacgtc gatctcggcg acggcgccga
      661 ggctgacgaa ctcgccaccg ggctgcggga tcggcaagtc cgtgatcgcc gccagtccgg
      721 ggcggatcgt ttcgggcagc ccgatgatca cctcgatttc gttcgagcgc ccctggaagc
      781 gacgtagatg ctggccgcgg aaggcgaaac cgaccgtgtc cgcgatcgtc tcgggctcga
      841 tgccgagcgc ccgcgcgcgc tccgcatcga tcagcacctg gacttcctcg cgtccgttca
      901 acgacggacc gtagatctcg tcgacgtgct cgatcccgcg cagccggcct tcgacgtcgt
      961 aggccagctc ctgtaggtac tcggggtcct cgccatggac cgcgacactc accattccgc
     1021 cgcggcgcgg cccgcggtgc cggccgccgt gttcgccgaa ctcgaactcc acgccggcga
     1081 ccgtcggcag cccccggcgc agcttgtctt cgagtcgctt gaacgccgtc tccgtgttct
     1141 tcccttccgg cagatagacc cgcgtgttgc agttgttctg cccttcggta taccaggaat
     1201 agatgtccga gtagcccagt tcttgcttgt tggcctcgag ccagtcctcg acgcggttga
     1261 cgtatccttc cagcacctcg aggctcgaag cgtcggccac ttggtagacg atcagcactt
     1321 cgcgttgctg catcttgggc tgtccggtct tctcgatcag tccgatcggg atcgcggcac
     1381 tcgcggccaa gccgaacagc gcgatcaggg tcagcgcgcg gtgctggagc gtccaaccca
     1441 ggatcgcgcg gtagcgtggc accagccggc ccacgaagaa cccccgccgg atcggctcgt
     1501 ccgagacgta gcgggctgcg gcgagcggga tgaacgtcat cgagatgatc agcgagcaga
     1561 cgaccgagat gcagatcacc gcggccaccg cgccgatgta gatcttcatc tcgttcggct
     1621 cgagcacgaa caaccacgac caaacgatga tggtggtcgc ggtggcggcc agcaccgcga
     1681 gcgccacctg tcgggccccg agccgggcgg cgcggtcggg cgacatgccc tgcgcctgca
     1741 gccggtagat gttctcgatc acgacgaccg cgttgtcgac cagcatgccg acgccgagca
     1801 tcaatccgag catggtcaag acgttgaact ccatgccgaa catgaacctc gccccgcagg
     1861 tgacgagcag cgagaacgga atggcgaccg ccacgatcaa cgtcgtcttg agccgtcgca
     1921 agaagaagta gagcacgccg acggcgagca acccgccgaa gatgccggca ttgcgcaggc
     1981 cgcgcaggga gttgcggatc tcctcgccct ggttttgcca gacgacgagg ttgattccgc
     2041 cgagctccgg atcgcgtccg atctcgtcga tcttggccat cagctgatcg accgtctcga
     2101 ccgtgttggc cgaggcctct ttgatcacgt cgaagccgat ggcgaagtct cgattgaggt
     2161 ggcgcccgta gtcgagcagc ggctcgcgca gctcgacccg cgccacgtcg cggagtcgca
     2221 ggcctcgcct gccgatgtcg aggtcgcgaa tcgtctcgat ttggtcgaac cgtgcctcgc
     2281 tgcgcacgtc gtagcgcagc tcggggccgc ggatcgcgcc gagg
//
LOCUS       DASPGN010000048         7750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_244887, whole genome shotgun sequence.
ACCESSION   DASPGN010000048 DASPGN010000000
VERSION     DASPGN010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244887"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VD788_01445"
     CDS             <1..651
                     /locus_tag="VD788_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74954.1"
                     /translation="FHPQRRRREMRRAAIVSAGCLLSAMFSGGCGPKYETVTYPPRID
                     LSQHELIGVIEFDSDEAGELAPLATRRFTESARRDQGPVRVVWLDAERRRPGPDRIRE
                     LGREHGLRTIVVGRITVSDVKPRVSVSQSIASGSLSALIEATLAVELIETATGASLWN
                     ASASAQDTVGNLSLVGGKSVSFDAQDPDDAYGGLIDALVAQVTRDFHATRGRQPIR"
     gene            complement(660..1589)
                     /locus_tag="VD788_01450"
     CDS             complement(660..1589)
                     /locus_tag="VD788_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HXV74955.1"
                     /translation="MDRASRIFVAGHRGMVGSAVVRLLRQSGYTGILTRTRDELDLLD
                     QRRVRRFFRELQPRYVVLAAARVGGIQANNDFQADFLYENLVIQTNVIGAAVDEGSDK
                     LLFLGSSCIYPRMATQPIDEAALLTGPLEPTNEGYAIAKIAGMKLCEKIVLQHGRRFV
                     SAMPTNLYGPGDDYRSDQAHVIPMLLRRIHRAKATGAPTVTIWGSGSPLREFLHVDDL
                     ARALLLMLERYEEPTHVNIGSGEEISIRELAALIAETVGYRGELVWDRSKPDGTPRKL
                     LDSSRIRALGWSPRIGLRDGLASTYREALAALD"
     gene            complement(1595..2599)
                     /gene="gmd"
                     /locus_tag="VD788_01455"
     CDS             complement(1595..2599)
                     /gene="gmd"
                     /locus_tag="VD788_01455"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXV74956.1"
                     /translation="MKKALITGITGQDGSYLAELLLDKGYEVHGIIRRSSSFNTRRID
                     HIYQDPHLNNVRLFLHFGDLTDSTNLNRLLRTVRPDEIYNLGAQSHVRVSFDIPEYTA
                     DVAALGTIRLLDGIVDAGLDTRFYQASSSEMFGTATEVPQRETTPFRPRSPYAYAKLF
                     AHWATVNYREAYGLHASSGILFNHESPRRGETFVTRKITAALARILKGEQQQLFLGNL
                     DARRDWGYAVDFVEAMWLMLQTEQPDDYVIATGETRSVREFLDAAFGLVDRDWNDYVR
                     IDERYFRPTEVDLLVGDAAKARARLGWHPRVGFDELVRLMVREDLRGVGLDPDRWLKE
                     "
     gene            complement(2641..3393)
                     /locus_tag="VD788_01460"
     CDS             complement(2641..3393)
                     /locus_tag="VD788_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HXV74957.1"
                     /translation="MGETRLRVYLFEGSNACLTCTLMLGHKRLAYERVDLPPALHALI
                     VRALGFPRTTVPAMWIGDRRVQGTLDISAALDREFPDEPLYPSDPAARSRVREAEVWG
                     EKLQNACRRIFYAAARRDHRVFPAFISRGGLSAATAWAVRAATPLIIRVAIVGHGASD
                     ERARRDLERLPDNLDRIDAYLASDVLGGERLNAADYQIAPNVRAMLQFADLAPLVDGR
                     PAAAWSMRVVPAYGRPVGAALPDDWIRPLRRR"
     gene            3537..4658
                     /locus_tag="VD788_01465"
     CDS             3537..4658
                     /locus_tag="VD788_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74958.1"
                     /translation="MVATYRFRVLDVAVALCGPDEVLRPIVRAYGRFRVSEANGSPPS
                     HRIRLEGLEADSFLDGGETVPRIRGASLTLQLYERFLNAIFDRVSSHAVLHAGALVDG
                     RGRALLISGPSGFGKTSLTLELLGRGLRFLSDDYAPLELASGRIQPYPRTIGLLPDGA
                     IRAPERFAEAAADPALPRLLGKALIDVGEVLGEQAIAGEPAPVGDVVVLDASGPARDD
                     RRSIVQFGVWPRGVADCAREIAGIPGVVVLGRHDRADITVWQVALDESKLPTPRFSQL
                     LERDFIAYSEVRPSVTPDFAAPPRLAPLTRREAALSLCREIQNRRPRGRLMQSYGGDT
                     TALFLDVAERLAPARCHRLEVGALRPTADLIERLARGTG"
     gene            complement(4675..5178)
                     /locus_tag="VD788_01470"
     CDS             complement(4675..5178)
                     /locus_tag="VD788_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74959.1"
                     /translation="MRTTVVVTAVLLAGLTAAPLGAQTGIGDEASPWGLRVGLATDPD
                     QVVGGVNFLETPVATNLFIVPNAEIGFGDDAIVLTGTAPVHYRFVVDGSIRPYAGGGV
                     TVGWIDIDLPDNANPNADDDGEFEIAFRGTGGIIWRLNSGTEMFAELNLIAGDLYDAQ
                     VMLGWRF"
     gene            complement(5293..7152)
                     /locus_tag="VD788_01475"
     CDS             complement(5293..7152)
                     /locus_tag="VD788_01475"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXV74960.1"
                     /translation="MRQVTAGITTPNRLVCLTALAAALALAGCGAHRAATAPDGATDL
                     TADGAIADAGEEAGDADAPPDIASLTTAPIEEPLVEPASDEIWSPLTDDPLAVVDEPA
                     APEATASDPAQLVAEALEAVDAARTSWEQGDISSALATLDSAYGLLLQIPDDEPDLTQ
                     EKEDLRHLISRRVVEIYRSRLTSAVDLASPIPVQVNAYVEREIQSFQGGERQFFIDSY
                     RRSGLYRPMIVRMLRERGLPEELSWLPLVESGFKTRALSRARALGMWQFISSTGYRYG
                     LTRSHWIDERMDPEKSTGAAIEYLTELHGMFGDWMLALAGYNCGENRVVSVIRRQRDD
                     YLDHFWDVFEQLPRETARYVPRFLATLLIVRNPEQYGFELPELLPPVGYETVSVTRHV
                     RLDELGRGLGLDNDVLVELNPELRRGVTPDTTYALRVPLAAAPTFQARLAELPVYTPP
                     EDTYTIHRVRRGETLSTIARRHGSSINAIVQANNLRSRNRIREGQRLRIPTQGATASA
                     PAGAAGTTGDPVSLTHSVRRGDSLWKLASRYGTTVDRIKRDNGLRSDRLYVGQRLRIS
                     SGPPVGSRTYSVQRGDTIGRIARAHSVSLHAVLQANGLSRSSTIYPGQVLIIP"
     gene            complement(7491..>7750)
                     /locus_tag="VD788_01480"
     CDS             complement(7491..>7750)
                     /locus_tag="VD788_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74961.1"
                     /translation="PHRRADSTAGQTGERSVAAAIERSLPLLVEDGERWLAGEVPVQA
                     GRGCVFCHHVGLALWSHREAVRSGVVSEDPSVADLRRRAVE"
BASE COUNT         1147 a         2703 c         2673 g         1227 t
ORIGIN      
        1 ttccacccgc agcgaaggag acgcgagatg aggcgagcgg cgattgtctc ggccggatgt
       61 ctgctctcgg cgatgttctc cggcggatgt ggcccgaagt acgaaacggt aacctacccg
      121 ccgcggatcg acctgtcgca acacgagctg atcggcgtga tcgagttcga ctccgacgag
      181 gccggcgagt tggcaccgct cgccacgcga cgattcaccg agtcggcccg gcgcgaccag
      241 ggaccggtcc gcgtcgtgtg gctcgacgcc gagcggcgac gcccggggcc ggatcgcatc
      301 cgcgagctgg ggcgtgagca cgggttgcgc acgatcgtcg tcgggcggat caccgtctcc
      361 gacgtcaagc cgagagtctc ggtctcgcag tcgatcgcgt ccggcagcct gtcggccttg
      421 atcgaggcga cgctcgccgt cgaactgatc gagaccgcga ccggcgcttc gttgtggaac
      481 gcgtccgcca gcgcgcagga cacggtcggc aatctgagcc tggtcggcgg caagtcggtg
      541 agcttcgacg cccaggatcc ggacgacgcc tacggcgggt tgatcgatgc gctggtcgcg
      601 caggtgaccc gcgacttcca cgccacccgg ggccggcaac cgatccgctg accgccgtct
      661 cagtcgagcg cggccagcgc ctcgcgatag gtcgaggcca ggccgtcgcg cagcccgatc
      721 cgcggcgacc aaccgagcgc gcggatgcgc gaggaatcga gcagcttgcg cggcgtcccg
      781 tcgggcttcg accggtccca caccagctcg ccgcgatagc cgaccgtctc cgcgatcagc
      841 gcggccagct cgcggatcga gatctcctcg ccggagccga tgttgacgtg tgtcggctct
      901 tcgtagcgct cgagcatcag cagcaacgcg cgggccagat cgtcgacgtg gaggaactcg
      961 cgcaacgggc ttccgctacc ccaaatcgtc accgtcggcg cgccggtcgc cttggcgcgg
     1021 tggatcctgc gcagcaacat cgggatcacg tgggcctgat ccgaccggta gtcgtcgccc
     1081 ggcccgtaca ggttggtcgg catcgccgag acgaagcgcc gcccgtgctg cagcacgatc
     1141 ttctcgcaca gcttcatccc ggcgatcttg gcgatcgcgt agccttcgtt ggtcggctcg
     1201 agcgggccgg tgagcagggc ggcctcgtcg atcggctggg tcgccatccg cgggtagatg
     1261 cacgagctgc cgaggaacag cagcttgtcg ctgccctcgt cgaccgcggc cccgatcacg
     1321 ttggtctgga tcaccaggtt ctcgtagagg aagtccgcct gaaagtcgtt gttggcctgg
     1381 atcccgccga cgcgtgccgc cgcgaggacg acgtaacgcg gctgcagctc gcggaagaag
     1441 cgtcgaaccc gccgctgatc gagcaggtcc agctcgtcgc gggttcgtgt gaggatgccg
     1501 gtgtatccgc tctgtcgcag gagccgtacg accgccgagc cgaccatccc gcggtgcccg
     1561 gcgacgaaga tgcgactcgc ccgatccatc ccggctactc cttgagccaa cggtccgggt
     1621 cgagaccgac gccccgcagg tcttcgcgga ccatcaaccg caccagctca tcgaagccga
     1681 cgcgcgggtg ccagcccagc cgagcccggg ccttcgccgc gtcgcccacc agcagatcga
     1741 cctcggtcgg ccgaaagtaa cgctcgtcga tccgcacgta gtcgttccag tcgcggtcga
     1801 ccaggccgaa ggccgcatcg aggaactcgc gcaccgagcg cgtctcgccg gtagcgatca
     1861 cgtagtcgtc gggctgttcg gtctggagca tcaaccacat cgcctcgacg aaatctacgg
     1921 cgtatcccca gtcgcgcctg gcgtcgaggt tgccgagaaa cagctgttgt tgctcgccct
     1981 tgaggatgcg tgccaacgcc gcggtgatct tgcgcgtgac gaaagtctcg ccgcgccggg
     2041 gcgattcgtg attgaacagg atcccgctcg atgcgtgcag gccgtaggcc tcgcggtagt
     2101 tgaccgtggc ccagtgggcg aacagcttgg cgtaggcgta ggggctccgc ggccgaaacg
     2161 gcgtcgtctc gcgttgcggg acctcggtcg ccgtgccgaa catctcggag ctcgaagcct
     2221 ggtagaagcg ggtgtcgagg ccggcgtcga cgatcccgtc gagcagccgg atcgtgccga
     2281 gcgcggcgac gtcggcggtg tactccggaa tatcgaagct gacccggacg tggctttgcg
     2341 ctcccaggtt gtagatctcg tcggggcgta ccgtccgcag caggcggttg aggttggtcg
     2401 aatcggtcag gtcgccgaag tgcaggaaca gacgaacgtt gttcaggtgc gggtcctgat
     2461 agatgtggtc gatccgtcgc gtgttgaacg agctcgatcg ccggatgatc ccgtggacct
     2521 cgtagccttt gtcgagcaac agctcggcga ggtacgaacc gtcctgaccg gtgatgcccg
     2581 tgatcagcgc ctttttcatc cccgccgtcc ctcgaccagt tcgccccgac ccatcctaac
     2641 tcatcgtcgg cgcagcggtc ggatccagtc gtcgggcagc gccgcgccga ccggccggcc
     2701 gtaggccggc acgacccgca tcgaccaggc ggctgccggc cgcccgtcga cgagcggcgc
     2761 caggtcggcg aactggagca tcgcgcgaac gttgggggcg atctgataat ccgccgcgtt
     2821 gagccgctcg ccgcctagca cgtcgctcgc gaggtaggcg tcgatgcgat cgaggttgtc
     2881 cggcaggcgt tcgaggtccc gccgagcgcg ctcgtcgctc gcgccgtgcc cgacaatcgc
     2941 gacgcggatg atcagcggcg tcgccgcacg cacggcccag gcggtcgctg ccgacaggcc
     3001 gccgcgtgag atgaatgccg ggaagacgcg atggtcccgc cgcgcggcgg cgtagaagat
     3061 cctgcggcac gcgttctgca gcttctcgcc ccagacctcc gcttcgcgca cccgcgaccg
     3121 cgcggcggga tccgacggat agagcggctc atcgggaaac tcgcggtcca acgccgccga
     3181 gatgtcgagc gtgccctgca cccgccggtc gccgatccac atcgccggaa ccgtcgtccg
     3241 cggaaagccg agtgcgcgta cgatcaaggc gtgcagcgcc ggcggcagat cgacccgctc
     3301 gtaggccagc cgcttgtggc cgagcatcag cgtgcaggtc aggcacgcgt tcgacccctc
     3361 gaacagatag acccgaagcc gagtctcgcc caccgtttgg ctctccgcgc ggcgcggcag
     3421 ccgctccgca cgcgccgacc gtacgccagc tcgacgccac gcgcaaccac cgcgcggccc
     3481 cggcgccgca cggtcacgtt gtccattgcg gcgagagcgc atagaatgcg cctccgatgg
     3541 ttgccaccta ccgttttcgc gtgctcgacg tcgccgtcgc gctctgcgga ccggacgagg
     3601 tcctgcgccc gatcgtgcgc gcgtacggtc ggttccgcgt gagcgaggcg aacgggtcac
     3661 cgccttcgca ccggattcgg ctcgaggggc tcgaggccga ctcgtttctc gacggcggcg
     3721 agacggttcc gcggatccgc ggcgcgagcc tgacgctcca gctctacgag cgattcctca
     3781 acgcgatctt cgatcgcgtc tcgagtcacg ccgtgctgca cgcgggcgcg ttggtcgacg
     3841 gccgcgggcg ggcgctgttg atctccggtc cgtcggggtt cggcaagacc tcgctgacgc
     3901 tcgagctgct cggtcgcggc ctgcgcttcc tcagcgacga ctacgcaccg ctcgagctcg
     3961 cgagcggacg gatccagccg tatccgagaa cgatcggtct gttgcccgac ggcgcgatcc
     4021 gcgcgcccga gcggttcgcc gaggcggcgg ccgacccggc gctgccgcgg ctgctcggca
     4081 aggcgctgat cgacgtcggc gaggtgctgg gcgagcaggc gatcgccggt gagcccgctc
     4141 cggtcggaga cgtcgtcgtg ctcgacgcga gcggcccagc ccgggacgac cgccgctcga
     4201 tcgtccagtt cggcgtctgg ccgcgcgggg ttgccgactg cgcgcgggag atcgccggga
     4261 tacccggcgt ggtggtgctc gggcgccacg accgcgccga catcacggtg tggcaggtcg
     4321 cgctcgacga aagcaagctc ccgacgccgc gcttctccca gctgctcgag cgcgacttca
     4381 tcgcgtactc ggaggtccga ccgtcggtga cgcccgattt tgccgcgccc ccgcgtcttg
     4441 caccgctgac ccgacgcgag gcggcgctgt cgctgtgccg cgagatccag aaccggcgac
     4501 cgcgcggccg gttgatgcag agctacggcg gtgacacgac cgcgctgttc ctcgacgtgg
     4561 ccgagcggct ggcgccggcg cgctgtcacc ggctcgaggt cggcgcgctg cgcccgacgg
     4621 ccgacctgat cgaacgactc gctcgcggga ccggctgagc cggccaccgg cgtctcagaa
     4681 gcgccagccg agcatcacct gtgcgtcgta caggtcgccg gcgatcaggt tcagctcggc
     4741 gaacatctcc gtgccgctgt tcaaccgcca gatgatcccg cccgtgcccc tgaacgcgat
     4801 ctcgaactcc ccgtcgtcgt cggcgttggg gttcgcgttg tccggcaggt cgatatcgat
     4861 ccagccgacc gtcacgcccc cgccggcgta ggggcggatg ctgccgtcga cgacgaaacg
     4921 atagtgcacc ggggccgtcc cggtcaggac aatcgcgtcg tcgccgaaac cgatctccgc
     4981 attgggtacg atgaacaagt tggtcgcgac cggtgtttcg aggaagttga caccaccgac
     5041 gacctggtca ggatcggtcg ccagcccgac gcgcagcccc cacgggctcg cttcgtcgcc
     5101 gatgcccgtt tgcgccccga gcggagccgc tgtcagcccc gcgagcaaga ccgccgtcac
     5161 gactaccgtg gttctcaccg ctcctcctcc ctgccattcg cgaccgcgaa acggggccgc
     5221 gtcacccgac gatctcgcat cctagcagcg attcggtcgc cgggaaacga aacggtgcgg
     5281 ttgccccggc gatcagggga tgatcagcac ctgtcccgga tagatcgtgc tcgagcggga
     5341 cagcccgttg gcctgcagca cggcgtggag cgacacgctg tgcgcccgcg cgatcctgcc
     5401 gatcgtatcg ccgcgctgca cgctgtaggt ccgcgaaccg accgggggtc cgctgctgat
     5461 tctcagccgc tggccgacgt agagccgatc gctccgcagg ccgttgtcgc gcttgatccg
     5521 gtcgacggtc gtgccgtagc gcgaggcgag cttccacaag ctgtcgccgc gccggacact
     5581 gtgtgtgagc gagacggggt cgccggtcgt cccggcggcg ccggccgggg cgctcgccgt
     5641 cgcgccttgc gtcgggatcc tgagccgctg accctcgcgg atgcggttgc ggcttcgcag
     5701 gttgttcgct tgcacgatcg cgttgatcga cgagccgtgg cgacgggcga tcgtcgacaa
     5761 cgtctcgccg cgtcgcacgc ggtggatcgt ataggtgtcc tccggcgggg tgtagaccgg
     5821 cagctccgcc agccgggcct ggaacgtcgg cgcggcggcg agcgggaccc gcagcgcgta
     5881 ggtcgtgtcc ggcgtgacgc cgcggcgcag ctcgggattc aactcgacga ggacgtcgtt
     5941 gtcgagcccg agcccgcgcc ccagctcgtc cagtcggaca tggcgcgtga cgctgaccgt
     6001 ctcgtagccg acggggggca acagctccgg cagctcgaac ccgtactgct ccgggttgcg
     6061 cacgatcaac agcgtggcga ggaagcgcgg cacgtagcgt gccgtctctc tcggcagttg
     6121 ctcgaacacg tcccaaaaat gatcgaggta gtcgtcccgc tggcgccgga tcaccgacac
     6181 cacacggttc tcgccgcagt tgtagccggc cagcgcgagc atccagtcac cgaacatccc
     6241 gtgcagctcg gtcaaatact cgatcgcggc gccggtcgac ttctccgggt ccattcgctc
     6301 gtcgatccag tgcgagcgcg tgagaccgta gcgataaccg gtcgacgaga tgaactgcca
     6361 catgcccaac gcgcgcgcgc gggacagcgc gcgcgtcttg aacccgctct cgaccagcgg
     6421 cagccagctc aactcctcgg gcagcccgcg ctcgcgcagc atgcgcacga tcatcggccg
     6481 gtagagaccg gagcgccggt aggaatcgat gaagaactgg cgctcgccgc cctggaagct
     6541 ctggatctcc cgctcgacgt aggcattgac ctgaaccggg atcggcgagg ccaggtcgac
     6601 cgcgctcgtc aggcgcgatc ggtagatctc gaccacgcgt ctcgagatca ggtgtctgag
     6661 gtcttctttt tcttgtgtca gatccggctc gtcgtcgggg atctgcagca gcagaccgta
     6721 ggccgaatcg agcgtggcga gcgccgagga gatgtcgccc tgctcccagg aggtgcgcgc
     6781 cgcgtcgacc gcttcgagcg cctccgccac caactgcgcc gggtcgctcg cggtcgcctc
     6841 gggcgccgcc ggttcgtcga cgacggcgag cggatcgtcg gtcagcggcg accagatttc
     6901 atccgacgcc ggttcgacga gcggctcttc gatcggcgcg gtggtcaacg aagcgatgtc
     6961 gggtggtgcg tccgcgtccc cggcttcctc gccggcgtcc gcgatcgcgc cgtcggcggt
     7021 gagatccgtc gcgccgtcgg gggcggtcgc cgcgcggtgg gcgccgcaac cggcgagcgc
     7081 gagcgcggcg gcgagcgccg tcagacaaac gagtcggttg ggagtggtga taccggccgt
     7141 gacttgtctc aatgcttcac ccgagtttcg tggttttggt cgaacgtggt cgtctcccgg
     7201 tcgaatctgg cggtcgagcc gcggccggtc aagtcttcca agttatccgc agccgcgccg
     7261 acccgcatac cggatcgacg cacggcacgc ggccgcacga cgggccgggc tcgcgagcga
     7321 gcccgtgctc cgggcgaccc ggtcgctatc tcattccgtt tcagtcggtt gcggctcgtc
     7381 gcgcgcgcga caggccgaca cgcggtcccc cagtagcggt agatgtagtc tcccgcgctc
     7441 gacggcgagg tgctggtctg ttcgctcggc gtgagccaac tcccgtcggg ttactcgacg
     7501 gcacggcggc gcaggtcggc gaccgacgga tcttcgctga cgacgcccga gcgcacggcc
     7561 tcgcggtggc tccacaaggc gagtccgacg tggtggcaga agacgcagcc gcggcccgcc
     7621 tggaccggga cctcgccggc gagccagcgc tcgccgtcct cgaccagcaa cggcaagctg
     7681 cgctcgatcg cggcggcgac cgatcgctcg ccggtctggc ccgccgtcga gtccgcccgc
     7741 cggtggggcc
//
LOCUS       DASPGN010000049         6504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_595750, whole genome shotgun sequence.
ACCESSION   DASPGN010000049 DASPGN010000000
VERSION     DASPGN010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6504
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595750"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(582..2201)
                     /locus_tag="VD788_01485"
     CDS             complement(582..2201)
                     /locus_tag="VD788_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV74962.1"
                     /translation="MSARSGDRAPLDDPLWALVHATSVHSGRRFFRELTRTLAAALGL
                     RIAAVSERIDGGRIRALSVWNCDDWDAEPEYLAAGSPCGVVIDSLRPQLIERGARDRF
                     PDDELLERWRIEGYAGAPLIDAGGRAIGLLCVMDPEPIDDDPRIREILEVFASRAALE
                     LDRSRALVELECQRAFLRQVLDIDPSLIFAKDRDGRFTLVNQALADVYGTTIEELIGK
                     TDADFNPKSEEVEFFRRMDLEVMDTLRERFIPEEPLTDACGALRWVQTIKRPIIDANG
                     ASNQVLGVATDITELKRTREQLLERQRRQHERVQAELDKVKQELVRKTRLAAIGQVAA
                     SIAHELRNPLGAISNSIFFLGRHYPGIDDKWRRHLDIIRQEVTASDRIVTNLLDMSRA
                     ADPVKESVELGGLVREVFDRLVGDQAVALHVDVEPDPFPVRADPAQLRLVLGNLILNA
                     LQSMPKGGHVWVTGRREAAGVVVTVRDAGVGIDATIREEVFEPLFTTKAKGTGLGLAI
                     CREIVERHKGSIRLLEVAGGAAFELRFPHDG"
     gene            2331..3284
                     /locus_tag="VD788_01490"
     CDS             2331..3284
                     /locus_tag="VD788_01490"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HXV74963.1"
                     /translation="MNASSRHPPDARWPELPYEPWKRTAATLQLWTQIVGKIRATLTP
                     WTNHSWHVTLYVSDRGLTTGPIVHGERTFSIEFDLIGRRLVVSTCDGERALVPLEPQT
                     TADFYRRTMRALHDLQLTVAIHRYPNEVPEPRRFDLDREHASYDAEAAERFWRVLLQV
                     DRVFRVFRAGFIGKVSPVHFFWGSFDLAVTRFSGREAPEHPGGIPNLPDWVTREAYSH
                     EVSSAGFWPGADALPQPIFYSYAYPAPDGFAGARVLPDAASWNAELGEFVLAYDAVRR
                     SSSPDRTLLDFLRTTYEAAADLGRWDRAALERTFDLPRRRA"
     gene            3325..4869
                     /locus_tag="VD788_01495"
     CDS             3325..4869
                     /locus_tag="VD788_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HXV74964.1"
                     /translation="MSPKRPAPAPRGGGRLIARTLGVIESVGNALPHPATLFAMLAAA
                     VVLASGLFHRIGVSAVHPGTKELVEPVSLISVWGLHEMLTKMVTNFTEFAPLGTVLVS
                     LLGIGIAERSGLIGTALRLLVLSAPRGLLTFVIVFAGILSNTASEVGYVLLVPLGAMI
                     FLAVGRNPIAGLAAAFAGVSGGYSANLLLGTLDPLLAGLSEEAARIIDPAYTVNPACN
                     WYFMFVSTFFIAAAGTWVTEKIIVPRLGDWKGDEQVEAIQTLGPREKRGLVRAAVVAA
                     LFVAFLLAGTVPDDGFLRDPRTGELLRSPFTGGIVAFIFIGAALTGVAYGWGARTLRN
                     DSDVIDGMAKSMNTLSVYVVLCFFAAQFVAYFKWTNLGLIFAIHGADLLGKSGLGDIP
                     LILAFVALSAIINLAMGSASAKWAIMAPVFIPMFMLLGYSPELVQATYRVGDSATNVI
                     SPLMSYFPLVVAFMKRYDPNAGIGTVVATMLPYSVTFFIVWTALLIVWFLLGIPVGPG
                     APLKIG"
     gene            4913..>6504
                     /locus_tag="VD788_01500"
     CDS             4913..>6504
                     /locus_tag="VD788_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV74965.1"
                     /translation="MQFLKFFGRYARRYTGWILLAAISIPLYGLGSAGMVAMIEPIFA
                     EVLLAGDAASEALPGLATRAEEAATETGPITGFNLRQLGNRGYRALKRAFGVDQTNVV
                     WFVPLLFFAVYLIRALMAFLSQYSFRHIGLGVVNDIRNELYRRLLAQSSSFHSTHPSG
                     ELISRLLTDVGMLQAAISNRLLDLLQESVTLIALLLLLLSTDFRLAVWCLVVTPLVLF
                     PLIRFGESMRRASRRGQERMAGVTALLSEGLRGHRVVKAFGMEEFEARRFARATRAHL
                     KVILRGQVISSVSSPVIESIAALGSVALFVYAGLQIRQGNLTAPLLVQFVANLMLMYD
                     PIRKLNNVNLVLQQARAAMKRVRALLDAPNEVTDRPGAITIDGVHQGIRFDGVHFGYG
                     TEPVLRGIDLDIRPGEIVALVGPSGAGKTSLVNLLPRFFDPTAGRVTLDGRDIRDITL
                     ASLRRQIGIVTQETMLFNDTIRDNIAYGRADVPLDQVREAARAAFADEFIMRQPAGYD
                     TVIGESGSHLSGGQRQRLSIARA"
BASE COUNT          945 a         2309 c         2150 g         1100 t
ORIGIN      
        1 ctatctcacc gacgatcgct ggttcgaggg gctgcgtgaa gacccgcgtt tcctggccct
       61 gctcgagcgc atgcgcggct cggccgggcg ggaatgaaac gggtgagccg acgcggctca
      121 gggtccgagt aatcggcgct acgtcacacc aagagtgcgg gaacgaggcg ctcgccaatc
      181 ctgacccggc tcatgtccat ccccttcttc ccgagacgtc aatcccgatg cgtcggagga
      241 agatcggatg cttcgcatcg cccaggaaca ggaatccgac aagtcgtggt cggccggacg
      301 atcagcggga agtcctcggt ccctggaccg gagtgtcgat ctcgccgacc cggtcgtggt
      361 ggcggagcga cgagccctcg atgtccttcc ggtagatctt gcctcccacg gaaccgttcc
      421 gtcgagccag tagaggcggt cgttccggat gtcgatcgcc atcgcgcgcg ggtccggggc
      481 gcgactcgtg tccaggaaat ggaccggccc ctcgcgcgcg gacccgtcga gattggcgcg
      541 cgagatccga tcggtgccgt gcccttcaag gccggggcct gtcacccgtc gtgcgggaat
      601 cgcagctcga acgccgcacc gcccgcgacc tcgagcaacc ggatcgaacc cttgtgacgc
      661 tcgacgatct cgcggcagat cgcgagaccg agaccggtcc ccttggcctt ggtggtgaac
      721 agcggttcga acacctcctc gcggatcgtc gcatcgatcc ccaccccggc gtcgcgcacc
      781 gtgaccacca cgccggcggc ctcacgccgt cccgtgaccc acacgtggcc gcccttcggc
      841 atcgactgca acgcgttgag gatcaggttg cccagcacca gacgtagctg ggccgggtcg
      901 gcccgcaccg ggaacggatc gggctcgacg tcgacgtgca gcgcgaccgc ttgatcgccg
      961 accagccgat cgaatacctc gcgcaccaga ccgcccagct cgaccgactc cttgaccggg
     1021 tcggccgcgc gggacatgtc gagcaggttg gtcacgatgc ggtccgacgc cgtcacctct
     1081 tgccggatga tgtcgagatg ccggcgccat ttgtcgtcga tcccgggata gtgacgcccg
     1141 agaaagaaga tcgagttgct gatcgcgccc aacgggttgc gcagctcgtg ggcgatgctg
     1201 gccgcgacct gtccgatcgc cgccaaccgg gtcttgcgca ccagctcctg tttgaccttg
     1261 tccagctcgg cctgcacccg ctcgtgctgg cggcgctgcc gttcgagcag ctgctcgcgc
     1321 gtgcgcttca gctcggtgat gtccgttgcc acgccgagca cttggttcga cgccccgttc
     1381 gcgtcgatga tcggtcgctt gatcgtctgc acccaacgca gcgccccgca cgcgtcggtc
     1441 agcggctcct ccggaatgaa ccgctcgcgc agcgtgtcca tcacctcgag gtccatccgc
     1501 ctgaagaact cgacctcctc cgacttcgga ttgaagtcgg cgtcggtctt gccgatcagt
     1561 tcctcgatcg tcgttccgta gacgtcggcc agcgcctggt tgaccagcgt gaaccgcccg
     1621 tcgcgatcct tggcgaagat caggctggga tcgatgtcga gcacctgtcg gagaaaggcc
     1681 cgttggcact cgagctcgac cagcgcgcgc gagcgatcga gctcgagcgc ggcgcgcgaa
     1741 gcgaagacct cgaggatctc gcggatgcgc ggatcgtcgt cgatcggctc gggatccatc
     1801 acgcacaaca gaccgatcgc tcgcccgccg gcgtcgatca acggcgcgcc ggcgtaaccc
     1861 tcgatccgcc atcgctcgag caactcgtcg tcgggaaacc gatcgcgggc cccgcgctcg
     1921 atcagctgcg gccggagcga atcgatgacg acgccgcacg gcgagccggc cgcgagatac
     1981 tcgggctcgg cgtcccaatc gtcgcagttc cagaccgaca gcgcgcggat ccgcccgccg
     2041 tcgattcgtt cgctgaccgc ggcgattcgt agaccgagcg ccgcggccag cgtccgcgtc
     2101 agctcgcgga agaagcgccg gccggagtgg accgaggtgg cgtggaccag ggcccacagc
     2161 ggatcgtcga gcggcgcgcg gtcacctgat cgagcactca tcggcgggct cccggaagct
     2221 gctccagagc ataacaccgg ccggcggccg actcggcgtg acccggcttg gatcgccggt
     2281 ccgctcgggt tatcatcacc gaccgctccg acagcgagcc gaggtccaga ttgaacgcga
     2341 gttcccgcca cccgccggac gcccgctggc ccgagctgcc gtacgagccc tggaagcgga
     2401 cggcggcgac gctgcagttg tggacgcaga tcgtcggcaa gatccgcgcg acgctgacgc
     2461 cgtggaccaa ccactcctgg cacgtcacgc tgtacgtctc cgatcgcggg ctgacgaccg
     2521 gcccgatcgt ccacggcgag cgcacgttct cgatcgagtt cgacctgatc ggccggcggc
     2581 tcgtcgtgtc gacctgcgac ggcgagcgag cgctcgtccc gctcgagccg cagacgacgg
     2641 ccgacttcta ccggcggacg atgcgtgcgc tccacgatct gcagctcacg gtcgcgatcc
     2701 accgctaccc caacgaggtg cccgagccac ggcgcttcga tctggatcga gagcacgcga
     2761 gctacgacgc cgaagccgcc gagcgattct ggcgcgtcct gctgcaggtc gaccgcgtct
     2821 tccgcgtctt ccgcgccggt ttcatcggca aggtcagccc cgtccacttc ttctggggca
     2881 gcttcgatct ggcggtcacg cgcttttccg gccgcgaggc gccggagcac ccgggcggca
     2941 tcccgaacct gcccgactgg gtcacacgcg aggcctactc gcacgaggtg tcgagcgcgg
     3001 gtttctggcc gggcgccgac gcgctcccgc agccgatctt ctactcctac gcttatccgg
     3061 cgccagacgg cttcgccggc gcgcgcgtgc tacccgacgc ggcgtcgtgg aacgccgagc
     3121 tgggcgagtt cgtcctggcc tacgacgccg tgcggcgatc gtcctcgccc gaccggacgc
     3181 tgctcgactt cctgcggacg acctacgagg ccgcggccga cctcggccgc tgggaccgcg
     3241 ccgcgctcga acggactttc gaccttcctc gccgccgcgc ttgagtcgcg accagcggcc
     3301 cgacgccggc cacggagacc accgatgagc ccgaagcgac ccgcacccgc gccccgaggc
     3361 ggcggtcgcc tgatcgcgcg tacgctcggg gtgatcgaga gcgtcggcaa cgcgctgccc
     3421 catccggcga cgttgttcgc gatgctggcc gcggcggtcg ttctggcatc gggtctgttc
     3481 caccggatcg gcgtgtcggc ggtgcatccc ggcaccaagg agctcgtcga gccggtcagc
     3541 ctgatcagcg tgtggggcct gcacgagatg ctgacgaaga tggtgaccaa cttcaccgag
     3601 ttcgccccgc tcggcaccgt gctcgtctcg ctgctcggca tcggcatcgc cgagcgcagc
     3661 gggctgatcg gcacggcgct gcggctgctg gtgctgtccg ccccgcgcgg cctgctgacg
     3721 ttcgtcatcg tcttcgccgg tatcctgtcg aacaccgcga gcgaggtcgg ctacgtgctg
     3781 ctcgtcccgc tcggcgcgat gatcttcctc gccgtcggcc gcaacccgat cgcggggctc
     3841 gccgccgcgt tcgccggggt gtcgggcggc tacagcgcca acctgctgtt ggggacgctc
     3901 gacccgctgc tcgccggatt gtccgaggag gcggcgcgca tcatcgaccc ggcgtacacg
     3961 gtcaacccgg cctgcaattg gtacttcatg ttcgtctcga ccttcttcat cgcggcggcc
     4021 ggcacgtggg tcacggagaa gatcatcgtg ccgcggctcg gtgactggaa gggagacgag
     4081 caggtcgagg cgatccaaac gctcggcccg cgggagaagc gcggcctggt ccgggccgcc
     4141 gtcgtcgccg cgctgttcgt cgccttcctg ctcgcgggca cggtgcccga cgacggcttc
     4201 ctgcgcgatc cgcgaaccgg ggagctgctg cgctcgccgt tcaccggcgg catcgtcgcg
     4261 ttcatcttca tcggcgccgc gctgaccggc gtggcctacg gctggggggc gcggacgctg
     4321 cgcaacgact cggacgtgat cgacggcatg gccaagtcga tgaacactct cagcgtctac
     4381 gtcgtgctgt gctttttcgc cgcacagttc gtcgcctact tcaagtggac gaacctcggc
     4441 ctgatcttcg ccatccacgg cgccgacctg ctcggcaagt cggggctggg cgacatcccg
     4501 ctgatcctgg cgttcgtcgc gctgtcggcg atcatcaacc ttgcgatggg cagcgcctcc
     4561 gccaagtggg cgatcatggc gccggtcttc atcccgatgt tcatgctgct cggctactcg
     4621 cccgagctgg tccaggcgac ctaccgcgtc ggcgacagcg cgaccaacgt gatctcgccg
     4681 ctgatgtcct actttccgct ggtggtcgcc ttcatgaagc gctacgaccc gaatgccggc
     4741 atcggcacgg tggtcgcgac gatgctgccc tactcggtca ccttcttcat cgtctggacc
     4801 gcgctgctga tcgtctggtt cctgctggga atcccggtcg gcccgggcgc gccgttgaag
     4861 atcggctgac gcgcggccgg gctgctacga tagcggcgcc cgggaggacg acttgcagtt
     4921 cctcaagttc ttcggccgct acgcccgacg ctacaccggc tggatcctgc tcgcggcgat
     4981 cagcatcccg ctctacggtc tcggctcggc cggcatggtc gcgatgatcg agccgatctt
     5041 cgccgaggtg ctgctcgccg gggacgccgc gtccgaggcg ctgccgggtc tcgcgacccg
     5101 cgccgaggaa gccgcaaccg agaccggccc catcaccggt ttcaacctgc gtcagctcgg
     5161 caaccgcgga taccgcgcgc tcaaacgcgc cttcggcgtc gaccagacca acgtcgtctg
     5221 gttcgtcccg ctgctgttct tcgcggtcta cctgatccgc gcgctgatgg ccttcctcag
     5281 ccagtactcg ttccggcaca tcgggctggg cgtggtcaac gacatccgca acgagctgta
     5341 tcgacggctg ctcgcgcagt cgagctcgtt ccactccacg cacccgtcgg gcgagctgat
     5401 cagccgcctg ctgaccgacg tcggcatgct gcaggccgcg atcagcaacc ggctgctcga
     5461 tctgctgcag gagtcggtca cgctgatcgc gctgttgctg ctgctgctgt cgaccgactt
     5521 ccggctggcc gtctggtgtc tcgtcgtcac gccgctcgtg ctgttcccgt tgatccgctt
     5581 cggcgagagc atgcggcggg ccagtcgtcg cgggcaggag cggatggcgg gggtcaccgc
     5641 cctgctgtcc gagggtctgc gcggccaccg cgtggtcaag gcgttcggca tggaggagtt
     5701 cgaggcgcgg cgcttcgccc gcgcgacgcg cgcgcacctg aaggtgatcc tgcgcggtca
     5761 agtgatctcg agcgtgtcga gcccggtgat cgaatcgatc gcggcgttgg gctcggtcgc
     5821 gctgttcgtc tacgccggct tgcagatccg ccagggcaac ctgaccgcgc cgctgctcgt
     5881 gcagttcgtc gccaacctga tgctgatgta cgacccgatc cgcaagctga acaacgtcaa
     5941 tctggtgctg cagcaggcac gcgccgcgat gaagcgggtg cgcgcgttgc tcgacgcacc
     6001 gaacgaggtc accgatcgtc cgggtgcgat cacgatcgac ggcgtgcacc aggggatccg
     6061 cttcgacggc gtccatttcg gctacggcac cgaacccgtg ctgcgcggga tcgacctcga
     6121 catccggccc ggggagatcg tcgcgctggt cggtccgtcg ggcgccggca agacgtcgct
     6181 ggtcaacctg ttgccgcggt tcttcgaccc gaccgcgggg cgggtcacgc tcgacgggcg
     6241 cgacatccgc gacatcacgc tggccagcct gcgcagacaa atcggcatcg tcacgcagga
     6301 gacgatgctg ttcaacgaca cgattcgcga caacatcgcc tacgggcgcg ccgacgtccc
     6361 gctcgaccag gtgcgcgagg cggcccgcgc cgcgtttgcc gacgagttca tcatgcgcca
     6421 gccggccgga tacgacacgg tgatcggcga gtcggggtcg cacctttccg gtgggcagcg
     6481 ccagcggctg tcgatcgccc gcgc
//
LOCUS       DASPGN010000050         6704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1064670, whole genome shotgun sequence.
ACCESSION   DASPGN010000050 DASPGN010000000
VERSION     DASPGN010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064670"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VD788_01505"
     CDS             complement(<1..205)
                     /locus_tag="VD788_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74966.1"
                     /translation="MHRISICSALVAAVAAFVTIDAADRTFAEIRAEGEARAFPLVVE
                     YFETDPRTRWDEVTSGAALERLRY"
     regulatory      complement(407..627)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            694..2112
                     /locus_tag="VD788_01510"
     CDS             694..2112
                     /locus_tag="VD788_01510"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HXV74967.1"
                     /translation="MSSPRIVCIALALALAGLVPAGCGPSDARPNVIVIMVDTLRADY
                     LGAYGFDGEVSPNVDALARESVQFDRCLAQGPWTAPSIASFLTSLHPQNHCVGNGYGL
                     FCEATDDEFVWSSLSERAVTLAEVFGAAGFDTAAFVGNWVVKSKFGFAQGFDSYFEPE
                     ASEQHVDNDAILFERARSWLEQRDASRPFLLYLHLMDVHAPYRVTHADIAVLRDSPSL
                     GDDREITDEEQSKRPGHLGLRIEWSSGAAQRSLRNWRTAYAAGVRGFDRGLGGFLDYL
                     RARGLLDDSIVVLTSDHGEEFLEHGEWEHGKTYYNEALRVPLLIRMPDLSLGGRRVDA
                     VVSLIDVMPTLIGLVGIDVERGTMAGTDLGRLWSGGQLPAAASFASALPQETASLAVQ
                     DRRWKLILDLETGSHRLFDLDHDPGELRDRSADESEQVARMKQRVEEHLETLERFPSF
                     RPTPTPLSDEDIERLRSLGYLR"
     gene            2124..2795
                     /locus_tag="VD788_01515"
     CDS             2124..2795
                     /locus_tag="VD788_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HXV74968.1"
                     /translation="MSVDRDSAGCGVYEGERLNSISHLCGAGLALVALIVLVVIAARQ
                     GDPWKIVSFAVYGASLVSLYLFSTLYHGVRGRAKRLFRRLDHNAIYLLIAGTYTPFVL
                     VTLRGGWGWSLFGVIWGLALVGIAGETLSRGAGKGLRVAVYLAMGWLIVIALRPLLER
                     LPDGAIALLAAGGVLYSVGVVFYALDKRIAHGHAIWHLFVIAGSAAHFFAVLLYVALL
                     PAEAG"
     gene            2792..3274
                     /locus_tag="VD788_01520"
     CDS             2792..3274
                     /locus_tag="VD788_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV74969.1"
                     /translation="MSTRAMLPILATLWLGSAVAADFDAGMAAYRAGDYQQAHDQWLP
                     LAEAGSAHAQFNLGLLYRYAKGREADPQQAAEWFLAAAESGLAAAQYELAEIYEAGEG
                     IERDVVEAHKWFKLAGEQKYDDAKKRRKRLASRMTSSEIALAEMWAREWKKTQKARAD
                     "
     gene            complement(3279..4775)
                     /gene="glpK"
                     /locus_tag="VD788_01525"
     CDS             complement(3279..4775)
                     /gene="glpK"
                     /locus_tag="VD788_01525"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HXV74970.1"
                     /translation="MARPCILSIDQGTTSSRAIAFDATPSIVALAQREFPQIYPAPGL
                     VEHDPEAIWSTTLEVASEVAAAVARRGFDVAAIGITNQRETTVVWDRETGRPIHNAIV
                     WQDRRTAEACERLRAAGLEPEIHARTGLLIDPYFSASKLRWLLDTVDGARAGAETGRL
                     AFGTIDTFLIWRLTEGTVHATDPTNACRTGLYNIHEMDWDDELLRIYDVPHRLLPEVR
                     DCDADFGVAAARLIGTAVPIRGVAGDQQAAAIGQCCFRDGDIKCTFGTGSFVVLNTGE
                     RPVASRNRLLTTVGSRIAGTTQYALEGSIFVAGAAVQWLRDGLGVIDDAAQTERLAAG
                     LADNGGVYLVPAFTGLGAPHWRADVRGAIFGLTRDSGPAQLARAALEAVGFQTHDLLR
                     VMAEDGVKPVALKVDGGMAANDWFVRFLAGVLDLPVDRPRIVETTALGAAYLAGRGAG
                     VFGSREQFADEWQREARFEPAWPRAERERLLAGWRESVDRLLYRPPPR"
     gene            complement(4788..5288)
                     /locus_tag="VD788_01530"
     CDS             complement(4788..5288)
                     /locus_tag="VD788_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74971.1"
                     /translation="MTETFKLKPIHRDAIDEALRKAEQYRLLNQPGQAESICRDILRV
                     DADNQRALTIIILALTDQFGRSHGSPSVKTAREYTAKLSDEYQRLYYGGLICERQARA
                     RLQRGMAGAFAYGGLREAMELYERAERVRPAGNDDAILRWNSCVRTIRRRNLEPQAAE
                     EEPLLE"
     gene            complement(5306..6064)
                     /locus_tag="VD788_01535"
     CDS             complement(5306..6064)
                     /locus_tag="VD788_01535"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HXV74972.1"
                     /translation="MTAGHRVFRIWRGDAHGGAFRDCTVEVDPGMVVLDVIHRIQAHE
                     LPDLAVRWNCKAGKCGSCSMEINGRPRLACMTRMNVFDPGETITVQPLKTFPVVRDLV
                     TDVSWNYEQNKRIPPFKPRPAGADGQHRIYQEDVDRVQEFRKCIECFLCQDVCHVLRD
                     RDGKRRFVGPRLMIRLAGLEMHPLDDDDRLEQIKNEFGSGMCNITKCCTDVCPEHIGI
                     TDNGIIPIKERVVDRYYDPLRVIAGRIFGRFGGG"
     gene            complement(6061..>6704)
                     /locus_tag="VD788_01540"
     CDS             complement(6061..>6704)
                     /locus_tag="VD788_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74973.1"
                     /translation="HGANRLGGNSLSDLIVLGCRAGRGAADYASGLSGSPRADDDQVV
                     AAFRRATQPLHRDAGENPYLLHDQLQQIMDAKVGIVRHAAELQQGIDQLAELGRRVEQ
                     VKAVGSSQYNPGWHEALAMRSLIVVAEAVALSAMTREESRGAHTRLDFDGERDEWLRF
                     NVVVRRADDGRMQVERVARPEPPAELARIAYAKIEDLEREIAAEATAAPRAGR"
BASE COUNT          978 a         2360 c         2287 g         1079 t
ORIGIN      
        1 tgtagcgcag ccgctcgagc gcggcgccgc tcgtgacctc gtcccaccgc gtgcgaggat
       61 cggtctcgaa gtactcgacc acgagcggga acgcgcgcgc ttcgccctcc gctcgaatct
      121 cggcgaacgt tcggtcggcc gcgtcgatcg tgacgaacgc ggcgaccgca gccactaacg
      181 cggaacagat cgagatccgg tgcatccatc tgcctcccgg acgaacgccg atcccgcggc
      241 gcgccggcca ggggccgatc gacggacgca gccgcagggc ggcgcccgtc gtgcaacgtg
      301 tgtcgcgaaa caaaaaaccc cggcggcgac cgcagcgccg cgcggggcgt tgttcggagc
      361 gctcgcgctc cacacactcg acggcggaac ctgcccctgg tcggttcagc ctttccggcg
      421 tgcgggtcgg tctcctggct cgcgggtcat ccggcttgtc gtgccttccc ggggtcgtta
      481 cgaccccagt ggcttacccc gtcgttccgt tgggacgaaa cgacgctcga cgattccgtc
      541 accgctcaca gtcgccgggc ggcaggggct tcgcacccct ttcccgttac ccgccacctc
      601 gtggcgcccc aggtataccc gaatgcggca cgcgaagcaa ccccggaggc ggcttgccgc
      661 cgatccggcg cctcgtatac tcctcggacg atcatgtcgt caccgcggat tgtgtgcatc
      721 gcgctcgcgc tcgcgctcgc gggactcgtc ccggccggtt gcggcccgtc cgacgcgcga
      781 ccgaacgtga tcgtcatcat ggtcgacacc ttgcgagccg actatctcgg cgcctacggc
      841 ttcgacggcg aggtctcgcc gaacgtcgat gcgctggcgc gcgagtcggt gcagttcgac
      901 cgttgcctgg cgcaaggtcc ttggaccgcg ccgtcgatcg cgtcgttcct gacctcactc
      961 cacccgcaga accactgtgt cggcaacggc tacgggctgt tctgcgaggc gaccgacgac
     1021 gagttcgtct ggtcgtcgct ctccgagcgc gccgtcacgc tcgccgaggt gttcggcgcc
     1081 gccggcttcg acacggccgc gttcgtcggc aactgggtcg tcaagtccaa gttcggtttc
     1141 gcccaaggtt tcgacagcta cttcgagccc gaggcgtcgg agcagcacgt cgacaacgac
     1201 gcgatcctgt tcgagcgggc gcgaagctgg ctcgagcaac gggatgcgag ccgtccgttc
     1261 ctgctctacc tccacctgat ggacgttcat gcgccctacc gcgtgaccca cgcggacatc
     1321 gcggtgctac gcgacagccc gagcctgggc gacgatcgcg agatcaccga cgaggagcag
     1381 tccaagcggc cggggcacct cgggctgcgc atcgagtggt cgtccggcgc cgcccaacgc
     1441 agcctgcgca actggcgcac ggcctacgcc gcgggtgtgc gcggcttcga ccgggggctc
     1501 ggcgggtttc tcgactacct gcgcgcccgc ggtctgctcg acgacagcat cgtcgtgctg
     1561 acttcggacc acggcgagga gtttctcgag cacggcgagt gggagcacgg caagacctac
     1621 tacaacgagg cgctgcgtgt cccgctactg atccgcatgc cggacctctc actcggcggc
     1681 cggcgggtcg acgccgtggt cagcctgatc gacgtgatgc cgaccttgat cggactggtc
     1741 gggatcgacg tcgagcgcgg gacgatggcc ggtaccgacc tggggcggtt gtggagcggg
     1801 ggccagctcc cggcggcggc cagcttcgcc tccgcgctgc cgcaggaaac cgcgagcctc
     1861 gcggtccagg accggcggtg gaagttgatc ctcgacctcg agaccggcag tcatcgtctg
     1921 ttcgacctgg accacgaccc cggcgagctg cgcgaccggt cggcggacga gagcgagcag
     1981 gtggcgcgga tgaaacaacg cgtcgaagag cacctcgaga cactcgagcg tttcccttcg
     2041 ttccgcccga cgccgacgcc gttgtccgac gaggacatcg agcgactccg ttcgctgggc
     2101 tacctgcgct agattcggct accatgtcgg tcgaccgcga ttcggcgggg tgcggagtgt
     2161 acgagggcga gcggctcaac agcatcagcc atctgtgcgg cgccgggctc gcgctcgttg
     2221 cgctgatcgt cctggtcgtg atcgcggcgc gccagggcga cccgtggaag atcgtcagtt
     2281 tcgccgttta cggcgcatcg ctcgtctcgc tctacctgtt ctcgaccctc taccacggcg
     2341 tgcgtggtag ggcgaaacgg ctgttccgcc ggctcgatca caacgcgatc tacctgttga
     2401 tcgccgggac ctacacccca ttcgtgctgg tgacgctgcg cgggggctgg ggctggtcgt
     2461 tgttcggtgt gatctggggg ctggcgctgg tcgggatcgc cggggagacg ctgtcgcgcg
     2521 gcgccggcaa agggctccgc gtcgcggtct acctggcgat gggctggctg atcgtgatcg
     2581 cgctgcgccc gctgctcgag cggctcccgg acggggcgat cgcgctgctc gccgcgggag
     2641 gcgtgctgta cagtgtcggc gtggtgttct acgcgctcga caagcggatc gcccacggcc
     2701 acgccatctg gcacctgttc gtgatcgccg gcagcgccgc ccatttcttc gcggtactgc
     2761 tctacgtcgc gttgctgccg gcggaggccg ggtgagcacc cgcgcgatgc tgccgatcct
     2821 cgccacgctc tggctggggt cggcggtcgc ggccgacttc gacgcgggga tggcggccta
     2881 ccgcgccggc gactaccagc aagcccacga ccaatggctg ccgctggcgg aggcgggctc
     2941 ggcgcatgcc cagttcaacc tggggctgct gtaccgctac gccaagggtc gcgaggccga
     3001 tccccaacaa gcggccgagt ggttcctcgc ggccgcggaa tccggtctgg cggcggcgca
     3061 gtacgagctg gccgagatct acgaggcggg cgaggggatc gagcgagacg tcgtcgaggc
     3121 gcacaagtgg ttcaagctcg ccggcgagca gaaatacgac gacgcgaaga agcgacgcaa
     3181 gagactcgcg tcccgcatga cctcctccga gatcgcactg gccgagatgt gggcgcgcga
     3241 gtggaagaag acgcaaaagg cgcgcgccga ctgacggatc agcgcggcgg cggccggtag
     3301 agcagccggt cgaccgactc gcgccagccg gcgagcaagc gctcgcgctc ggctcgcggc
     3361 caggcgggct cgaagcgcgc ctcgcgctgc cattcgtcgg cgaactgctc gcgcgacccg
     3421 aacacaccgg cgccgcggcc ggccaggtag gccgcaccga gcgcggtggt ctcgacgatc
     3481 cgcggccggt cgaccggcaa gtcgagcacg ccggccagga agcgaacgaa ccagtcgttg
     3541 gccgccatcc cgccgtcgac cttgagcgcg accggcttca cgccgtcctc ggccatcacc
     3601 cgcaacaggt cgtgggtctg gaaaccgacg gcctcgagcg ccgcgcgcgc gagctgcgcc
     3661 ggcccgctgt cgcgcgtcag cccgaagatc gccccgcgca cgtccgcccg ccaatgcggc
     3721 gcgcccaacc cggtgaacgc cgggacgagg tagacgccgc cgttgtcggc cagcccggcg
     3781 gcgaggcgtt cggtctgggc cgcgtcgtcg atcacgccga gcccgtcgcg caaccactgg
     3841 accgccgcgc ccgcgacgaa gatcgagcct tcgagcgcgt actgcgtggt accggcgatc
     3901 cgactaccga cggtcgtcag caaccggttg cgcgaggcga ccggccgctc gccggtgttg
     3961 agcacgacga agctcccggt gccgaacgtg cacttgatgt cgccgtcacg gaagcagcac
     4021 tgcccgatcg ccgccgcctg ttggtcgccg gccaccccgc gaatcggcac ggcagtgccg
     4081 atcagccgtg cggcggcgac gccgaagtcg gcatcgcagt ctcgcacttc gggcagcagg
     4141 cggtgcggca cgtcgtagat gcgcagcaac tcgtcgtccc agtccatctc gtggatgttg
     4201 tacaacccgg tgcgacaggc gttcgtcggg tcggtggcgt ggaccgtgcc ttcggtcagg
     4261 cgccagatca ggaaggtgtc gatcgtaccg aaggcgagcc ggccggtctc ggcgccggcc
     4321 cgagccccgt cgaccgtgtc gagcagccag cgcagcttgc tcgccgaaaa gtacggatcg
     4381 atcaacaaac cggtgcgcgc gtggatctcg ggctcgagcc ccgcggcccg caatcgctca
     4441 cacgcctcgg cggtgcgccg gtcctgccag acgatcgcgt tgtggatcgg ccggccggtc
     4501 tcgcggtccc agacgaccgt cgtctcgcgc tggttggtga tgccgatcgc ggcgacgtcg
     4561 aacccgcggc gagcgaccgc cgcagcgacc tcgctcgcga cctcgagcgt cgtcgaccag
     4621 atcgcctcgg gatcgtgctc gaccaggccc ggcgcggggt agatttgcgg aaactcacgc
     4681 tgggccagcg cgacgatcga cggcgtcgcg tcgaacgcga tcgcccggct gctcgtcgtg
     4741 ccctggtcga tcgacaggat gcagggtcgg gccaacggcc gctccgtcta ctcgagcagc
     4801 ggctcttctt ccgccgcctg cggctcgagg ttgcgccggc ggatcgtcct cacacagctg
     4861 ttccagcgca ggatcgcgtc gtcgttgccg gcggggcgca cccgctcggc ccgctcgtac
     4921 agctccatcg cctcgcgcag cccgccgtag gcgaacgcgc ccgccatccc gcgctggagc
     4981 cgggcgcgcg cctgtcgctc gcagatcaag ccgccgtagt acagacgctg gtactcgtcc
     5041 gacagcttgg ccgtgtactc ccgcgcggtc ttcacgctgg gcgagccatg cgaccgcccg
     5101 aactggtcgg tcaacgccag gatgatgatc gtcaacgcgc gctgattgtc ggcgtcgacc
     5161 cgcaggatgt cacgacagat gctctcggcc tggcccggtt ggttcagcag ccggtactgc
     5221 tcggccttgc gcaacgcctc gtcgatcgcg tcgcgatgga tcggtttgag cttgaacgtc
     5281 tctgtcaccg tgaccccctt tcgagtcagc cgccgccgaa gcgtccgaag atccggccgg
     5341 cgatcaccct cagcgggtcg tagtagcgat cgaccacccg ttccttgatc ggaatgatgc
     5401 cgttgtcggt gatgccgata tgctccggac agacgtcggt gcagcacttg gtgatgttgc
     5461 acatccctga cccgaactcg ttcttgatct gttccaaccg atcgtcgtcg tcgagcggat
     5521 gcatctcgag gccggccaat cggatcatca gccgcggacc gacgaaccga cgtttgccgt
     5581 cgcggtcgcg cagcacgtgg caaacgtcct ggcacaggaa gcactcgatg cacttgcgga
     5641 actcctgcac gcggtcgacg tcttcctggt agatgcggtg ctggccgtcg gcgccggcag
     5701 gccgcggctt gaacggcgga atccgcttgt tctgctcgta gttccacgag acgtcggtga
     5761 ccaggtcgcg gaccaccggg aaggtcttca acggttggac ggtgatcgtt tcgccggggt
     5821 cgaacacgtt catccgcgtc atgcacgcca gccgcggcct gccgttgatc tccatgctgc
     5881 acgaaccgca cttgccggcc ttgcagttcc agcggacggc gaggtccggc agctcgtggg
     5941 cctggatgcg gtggatcacg tcgagcacga ccatcccggg atcgacctcg accgtgcagt
     6001 cgcggaacgc cccgccgtgc gcgtcgccgc gccagatgcg aaacacccgg tgcccggccg
     6061 tcatcgtccg gcccgtggcg cggcggtcgc ctccgcggcg atctcgcgct cgaggtcttc
     6121 gatcttggcg taggcgatac gcgccagctc ggccgggggc tccggccgcg cgaccctctc
     6181 gacctgcatc cgcccgtcat ccgcgcgccg aacgacgacg ttgaatctca gccattcgtc
     6241 gcgctcgccg tcgaagtcga gacgcgtgtg ggcaccgcgg ctttcctcgc gtgtcatcgc
     6301 cgagagcgcc accgcctccg cgacgacgat cagcgaccgc atcgccagcg cctcgtgcca
     6361 gcccggattg tattggctcg aaccgaccgc cttgacctgt tcgacgcgcc ggccgagctc
     6421 ggcgagctgg tcgatcccct gctgcagctc ggcggcgtga cggacgatgc cgaccttggc
     6481 gtccatgatc tgctggagct ggtcgtgcag caggtagggg ttctcgccgg cgtcgcggtg
     6541 gagcggctgc gtcgcccgcc ggaacgccgc gacgacctga tcgtcgtcgg cgcgcggcga
     6601 gccggacaag ccacttgcgt agtccgccgc gccgcgtccg gcgcgacagc ccagtacgat
     6661 caggtccgac aacgagttgc cgcccaggcg gttcgcgccg tgca
//
LOCUS       DASPGN010000051         9859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1298806, whole genome shotgun sequence.
ACCESSION   DASPGN010000051 DASPGN010000000
VERSION     DASPGN010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1298806"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..1915
                     /locus_tag="VD788_01545"
     CDS             233..1915
                     /locus_tag="VD788_01545"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV74974.1"
                     /translation="MSCKIAPGLFVTVCCLLAGAPLIAEERFPDTGTGAQLFEGLGTY
                     HREFTTVSDEARQYLDQGMVWLQAFNYDEAERSFREAARLDPGCAMAWWGVAYAAGPS
                     YNHAQMSDQRAETCHEAIQNARARIENTSPVERALIEALSERYEMPYPIEDDSRLDQA
                     YADAMAKVWDQFSDDADVGMLYAEALMVRTPWALYNIDREPAEDTPMIEALLERVLEL
                     NPNHAGAAHLYIHAVELSESPERGLVVADRLSTLVPLSGHLLHMPSHIYTRTGDWHRA
                     IEQNALAMEADVEYRAKSPDQLVQHMYMVHNNHIRAYGAMMVGREAEALEASRRMWSQ
                     IPADLLEEVAHEVDAWMCSVYDVYKRFGRWDEMLAEPAPPDYMPFTLATWHAHRAISY
                     AAKREFDAAKREFTAYRRVRDNIRPEHLSNSYSVKAARGRLKVIEHFVPGEIALQQGK
                     LRTAIRHLERARIAEDKLGYSGEPPDYLQPIRHTLGAVYMMAGHLDKAEKAYREDLAE
                     FPANGWSLYGLSRVLRRQGRTEDAEQLEEEFRQAWAEADGPITTSCRCIPRI"
     gene            complement(1986..3797)
                     /locus_tag="VD788_01550"
     CDS             complement(1986..3797)
                     /locus_tag="VD788_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HXV74975.1"
                     /translation="MHEAGFNDLRQFIERLRRDGDLAVVEAEVDPRLEVAEIHRRVIA
                     ADGPALLFRNVRGAGFPLATNLFGTARRAELAFGKRPERLIRRLVELVERAIPPTVGK
                     LWGARDVARDLLRVGTRKRRSGPVCAVSTREVDLGRLPALTCWPEDGGPFITLPLVYT
                     EHPDTGGPNLGIYRLQVHDRRSTGMHWQIGKGGGFHYAVAEGRGDDLPATVFLGGPPA
                     LILAAIAPLPENVPELLLASLIAGRRLSTCDGPGAHRLLADAEFALSGRVPAGERRPE
                     GPFGDHYGYYSLRHDYPVFRVEEISHRRDAIFPATVVGKPRQEDFFIGDLLQELLSPL
                     FPLVMPAVEQLWSYGETGYHSLAAAVVKQRYKREAMVSAFRILGEGQLSLTKFLIVLD
                     RPTDLRDFPGTLVRLLERTTPESDLYVLANLSMDTLDYTAPRVNEGSKAVWLGLGEPR
                     RSLPRAFEAGVAPPPEVTDVRVFCPGCLVVGGPSRVDDERAAERLARHRAFADWPLVV
                     LTDEPRRAARSAVNFLWTTFTRFEPAADIHAAETRVIRNHLAFSPPILIDARLKPGFP
                     DELFADEVTAATVTRRWPEYFPGGQEMGDSSQAHLTR"
     gene            4174..5379
                     /locus_tag="VD788_01555"
     CDS             4174..5379
                     /locus_tag="VD788_01555"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HXV74976.1"
                     /translation="MKRKPPRPTDGEPPEPLASFAERREFCQFGERDAELLAELAPTF
                     ELAVPGLVDRFYEHLVGTPALRRFLDQPATVERLKKAQVAYLLSLTHGRYDDDYAASR
                     LRIGQVHERIGLEPQWYLGGYGPQIDMLLPHVHRHFAHRPADAVRASTALCKLIFLDM
                     QLALDAYYGIRRRKELSRSEQLAAVGELAASIAHEVRNPLAGMKGALQVLRGELAVKP
                     QNLEVVDELLAQIARLEDLVKDLLTFARPSSVSIRQFELQEVLDRLLRMYKDEADAAR
                     ITIDRNYAPDSRWVSADPLQMEQVFFNLIHNAFQAMEDGGRLTVSTTKNAGGVVIRFR
                     DTGKGIVPGDLTRIFQPFFTTKHRGSGLGLPIVKKIIDAHGGTIEVSSKPGQGTVATI
                     TLPGSGEVE"
     gene            5376..6776
                     /locus_tag="VD788_01560"
     CDS             5376..6776
                     /locus_tag="VD788_01560"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV74977.1"
                     /translation="MMAHENILIVEDEKLIRWSIKSRLEDEGYTVAEVDTGKKAFDLW
                     DQGDFDLMLLDFRLPDTTGVEILRRVKQQSPETSVVMMTAYGTVESAVEAMKLGAFDY
                     LTKPVNLEELVVIVEKALETTSLRREVRRLRNEHREIHGNVQLIGRSRAMLEIIELVE
                     KVCASQATTVLLEGDSGTGKNVVAKAIHYGSPRVDKPFVTITCSALTETLLESELFGH
                     ERGAFTDAKTQKKGLLEVADGGTAFLDEIGEMGMAMQAKLLRFLEEKTFKRVGGTRDI
                     EVEVRIIAATNRDLHEAVREGRFREDLYYRLKVIPIRLPSLRERRDDIPLLVQHFLDH
                     FNQEFRKNTTRVEPAAMERLKAYDWPGNIRELRNFIERVMILESKECIELADLPRAMR
                     DGLSAVEPTPAADSDPSPISLGAMTLEEMERAAIVQALEQVSQNQVRAAKLLGITRDT
                     LRYRMKKFGLLDGLRE"
     gene            complement(6793..7908)
                     /locus_tag="VD788_01565"
     CDS             complement(6793..7908)
                     /locus_tag="VD788_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV74978.1"
                     /translation="MKSSVVTFPIPPGAPRRQTDAAAPGGHDALPVYLRDMSAIPLLD
                     ARRERELFAALDRAKTGLIALGAELPARYRARRASQPHDADGAHASWPLERAERVHHG
                     YERWARERPREADAERVARARRLRHQLESAKEELILANLRLVVHLAKRYAGHGVGLLD
                     LIQEGNIGLMKAIEKFEYSRGNKLSTYAHWWIKQSIVRALADKSRLIRLPVHLHDRRR
                     KVTQTATLLGQTLGRDPLPIEIARKLSLSLEAVEKVLRMIAEPVALEDERGEESGPSP
                     VDTVEDTTAVSPWVQLAHRDMSARVEQTLQSLPPREEQVLRLRFGIGDDRPYTLDEIG
                     RILRVSRERVRQIQAAAIRRLKASHELDDYRDLRLEA"
     gene            complement(8135..8500)
                     /locus_tag="VD788_01570"
     CDS             complement(8135..8500)
                     /locus_tag="VD788_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV74979.1"
                     /translation="MRVIPTSILVVDDEKLIRWSLREHLRHAGYDVVLADSGRETMKT
                     LDERVALVLLDLRLPDVGGLELCDEILRRRPGCRVIMMTAQWTPELVREALAHGVSDV
                     LQKPFDLDEMTRAVATALA"
     gene            complement(8559..>9859)
                     /locus_tag="VD788_01575"
     CDS             complement(8559..>9859)
                     /locus_tag="VD788_01575"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HXV74980.1"
                     /translation="MNWLDDYKAKLRQPAEAVARITSHSNVYYGGNAAIPSALVRALA
                     ERRDELRDVTLHHVLLLGDDPLSSPGMEGHFRHNSLFVGPADRAAVNEGRADYLPIFL
                     HQIPRIFLEGRVELDVAMVMLSPPDEHGFMSLGVETLASKTACRAAKTVIAQVNEQMP
                     RVLGDSFVHVSRVDAIVERNEQLPTLIAKPATAVEIAIARNVVELIEAGSTIQMGIGG
                     IPDAVYAAIDGPLDLGIHTEMISDGAMRAIERGVVTGNRKTLHPGKAVITFALGSREL
                     YDFLDNNPFIEAHPVEHVNDPFVVGQNDNLVAVNSAIEIDLTGQVCSDSIGPRIYSGF
                     GGQVDFIRGAARSKGGKPIIAIPSTAQRGEASRIVPFLKEGAGVVTSRADVHYVVTEH
                     GVTNLFGKNLRERVEALIGIADPRFQQDLENAARERRLLP"
BASE COUNT         1730 a         3298 c         3311 g         1520 t
ORIGIN      
        1 cgaacgacgc gagccatccg atcctccctt ggcgctacgc gatggggatt gcaggaaaaa
       61 aacgtgaacg tgaactccgc gcggagcata taccaagtgg ggttcgcttg ttgctgagtg
      121 cgtctgcgcg tcgacccatg aggatggcca gcgggaggta tctggcatcc ccgaacttcg
      181 agagctttac ccgaccccgc gacgtgccgt ctcgcagggg tatactctgc gaatgagctg
      241 caaaatcgca ccgggccttt ttgtgaccgt ttgctgtctc ctcgccggcg ctccgttgat
      301 cgcggaagag cgattcccgg acaccggcac cggcgctcag ctcttcgagg ggctcgggac
      361 ctatcatcgg gagttcacga cggtgtccga cgaggcccgg cagtacctgg accaaggcat
      421 ggtctggctg caggccttca actacgacga ggccgagcgc tcgttccgcg aggcggctcg
      481 gttggatccc ggctgcgcga tggcatggtg gggcgtcgcc tatgccgcag gccccagcta
      541 caaccacgca cagatgtccg accaacgtgc cgagacgtgc cacgaggcga tacagaatgc
      601 ccgggcccgc atcgagaaca cgagcccggt cgaacgtgct ctcatcgagg cgttgagcga
      661 gcgttacgag atgccctacc cgatcgagga cgattctcgc ctcgatcagg cttacgccga
      721 tgcgatggcg aaggtctggg accagttttc cgatgatgcg gacgtcggca tgctctacgc
      781 cgaagcgctg atggtccgaa cgccctgggc gctctacaac atcgatcgcg agccggcgga
      841 agacacgccg atgatcgaag cactcctcga acgcgtgctg gagctgaacc cgaaccacgc
      901 cggcgcggcg cacctttaca tccacgccgt ggagttgagc gagtcgcccg aaaggggcct
      961 ggttgtggcg gaccggctca gcaccctcgt ccccctgagc gggcatctgc tgcacatgcc
     1021 ctcgcacatc tacacgcgca caggcgactg gcaccgcgcc atcgagcaga atgccctggc
     1081 catggaagcc gacgtggagt atcgggccaa gtcacccgat cagctcgtgc agcacatgta
     1141 catggtgcac aacaaccaca tccgggcgta cggcgcgatg atggtcggcc gcgaagcgga
     1201 agcgctcgag gcctcgcggc ggatgtggtc gcagatcccg gcggaccttc tggaagaggt
     1261 tgctcacgag gtggacgcct ggatgtgttc cgtctacgac gtctacaaac gctttggtcg
     1321 ctgggacgag atgctggccg agcccgctcc gccggactat atgcccttca cgctcgccac
     1381 ctggcacgcc caccgtgcca tctcgtatgc cgcgaagaga gaattcgatg cggccaagcg
     1441 agaattcacg gcttaccgtc gcgttcgcga caacatccgg ccggagcatc tgtccaactc
     1501 ctactcggtc aaggctgccc gagggcgtct caaggttatc gagcacttcg tgccgggaga
     1561 gattgccctg caacagggga agctgcgcac ggccatccga cacttggaac gggcgagaat
     1621 cgccgaggac aagctgggct acagcggcga acctccggat tacctccaac cgatccggca
     1681 caccctgggc gcggtctata tgatggccgg gcacctcgac aaagctgaga aggcctaccg
     1741 agaggacctc gcggagtttc ctgccaacgg ttggtcgctc tacggcctga gccgcgtcct
     1801 tcgtcgccag ggacggaccg aggacgccga acagctggaa gaagagttcc gccaggcctg
     1861 ggccgaagcc gacggaccga tcaccacgag ctgccgctgc attccgcgga tctagccggc
     1921 cgacccggac agggtcctgc gggcccgtca gcgggtggta cgagtgcagg cacggctgaa
     1981 agtgattagc gcgtaagatg cgcctgggaa gagtctccca tttcctggcc gccgggaaaa
     2041 tactccggcc agcgccgcgt gacagtcgcc gcggtcacct cgtcggcgaa cagctcgtcg
     2101 gggaaccccg gcttcagtcg cgcgtcgatc aggatcggcg ggctgaacgc gagatggttg
     2161 cggatgaccc gcgtctcggc ggcgtggatg tcggcggccg gttcgaaccg ggtgaaggtc
     2221 gtccacagga agttgaccgc gctccgcgcc gcacgtcgcg gctcgtcggt caacacgacg
     2281 agcggccagt cggcgaacgc cctgtgccgc gcgaggcgct cggccgctcg ctcgtcgtcg
     2341 acgcgcgacg gaccgccgac gaccaggcag cccggacaga agacccgcac gtcggtcacc
     2401 tccggcggcg gcgccacgcc cgcctcgaac gcccgcggca gcgaccggcg cggctcgccc
     2461 agaccgagcc acaccgcctt cgatccttcg ttcacccggg gcgcggtgta gtcgagggtg
     2521 tccatcgaca gattggccaa cacgtacagg tcgctctccg gcgtcgtgcg ttcgagcagc
     2581 cgcacgagcg tcccggggaa gtcgcgcagg tcggtcggcc ggtcgaggac gatcaagaac
     2641 ttggtcagcg acagctgtcc ctcgcccagg atacggaacg ccgagaccat cgcctcgcgc
     2701 ttgtaccgtt gcttgaccac cgccgccgcc agcgagtggt aacccgtctc gccgtaggac
     2761 cacagctgct cgacggccgg catcaccagc ggaaacaacg gcgacagcag ctcctgcagc
     2821 aggtcgccga tgaagaagtc ctcctgccgc ggtttgccga cgacggtggc ggggaagatc
     2881 gcgtcgcggc ggtgcgagat ctcctcgacg cggaagaccg ggtagtcgtg acgcagcgaa
     2941 tagtaaccgt agtggtcgcc gaacggccct tcgggccggc gttcgcccgc cgggacgcgc
     3001 ccgctcagcg cgaactcggc gtcggcgagc agacggtggg cccccggtcc gtcgcaggtc
     3061 gaaagccgcc gcccggcgat cagcgacgcg agcagcagct ccggcacgtt ctccggcagc
     3121 ggtgcgatcg cggccaggat caacgccggc gggccgccga ggaagacggt cgccggcagg
     3181 tcgtcgccgc gaccctcggc caccgcgtag tggaagccgc cgcccttgcc gatctgccag
     3241 tgcatgccgg tcgaacggcg atcgtggacc tgcagccggt agatgccgag attgggacca
     3301 ccggtatccg ggtgctcggt gtagaccaac ggcagcgtga tgaacgggcc accgtcctcg
     3361 ggccagcagg tcaaggcggg cagccgaccg aggtcgacct cgcgggtcga gacggcgcac
     3421 accggccccg agcggcgctt ccgcgtgccg acccgcagca ggtcccgcgc gacgtcgcgc
     3481 gcgccccaca gcttgcccac cgtcggcggg atcgccctct cgaccagctc gaccaggcgc
     3541 cggatgagac gctccgggcg tttgccgaag gccagctcgg cccgcctcgc ggtcccgaac
     3601 aggttggttg cgagcgggaa gcccgcgcca cggacgttgc ggaacagcag agccggtcca
     3661 tcggcggcga tcacccggcg gtggatctcg gccacctcga ggcgcgggtc gacctccgcc
     3721 tccaccaccg ccaaatcccc gtcacgccgc agccgctcga tgaactggcg cagatcgttg
     3781 aagccggcct cgtgcatggc accggatcct acacagcccg caccacggcc gcaaaagggc
     3841 agggcgcccc cttcgtcatg cagcgaaggg gacgccggag gagggaaggg agaacaatcg
     3901 ggggaagtcc ccggcactca gggcccttga gagagaagcg gtcagaagag gactggcact
     3961 gcggggactt ccccttccgg ccgacacaga cagcaaatcc cgggccatcg gtcggacccc
     4021 cgagaagttc gcgatttcgc gtcgagccgg caacggctcc caccgaccct gtgagcgatg
     4081 tcgcccaact gcggcattct gctcagcctg cggccgatcc tgctaacctc gtatcgaccg
     4141 agcctccccg ctcggtccgg cggaaggagt gacgtgaagc ggaagccgcc cagaccgacc
     4201 gacggcgaac cgcccgagcc gctggcctcg ttcgccgagc gccgcgagtt ctgccagttc
     4261 ggcgagcggg acgccgagct gttggccgag ctggcgccga ccttcgagct cgcggtgccc
     4321 gggctcgtcg accggttcta cgagcacctg gtcgggacgc cggcgctgcg ccggttcctc
     4381 gaccagcccg cgacggtcga gcgcctcaag aaagcgcagg tcgcctattt gttgagtctg
     4441 acgcacgggc gctacgacga cgactacgcc gcgagccgcc tgcgcatcgg ccaggtccac
     4501 gaacggatcg gcctcgaacc gcagtggtac ctcggcggct acggcccgca gatcgacatg
     4561 ctgctcccac acgtccatcg ccacttcgcg caccgccccg cggacgcggt gcgcgcgtcg
     4621 acggcgctgt gcaaattgat cttcctcgac atgcagctcg cgctcgatgc ctactacggc
     4681 atccgccgtc gcaaggagct gagtcgctcc gagcagctgg cggccgtcgg cgagctcgcg
     4741 gccagcatcg cacacgaggt ccgcaacccg ctcgccggca tgaagggcgc gctgcaggtg
     4801 ctgcgcggcg agctggccgt caagccgcaa aacctcgaag tggtcgacga gctgttggcg
     4861 cagatcgcac gtctcgagga cctggtcaag gacctgttga ccttcgcgcg tcccagctcg
     4921 gtctcgatcc gccagttcga gctgcaggag gtgctcgatc gattgctgcg gatgtacaag
     4981 gacgaggccg acgcggcgag aatcacgatc gaccgcaact acgcacccga tagccggtgg
     5041 gtctcggccg acccgttgca gatggagcag gtcttcttca acctgatcca caacgcgttc
     5101 caggcgatgg aggacggcgg caggctgacc gtctcgacga cgaagaacgc gggcggagtc
     5161 gtgatccggt tccgcgacac gggcaagggc atcgtgcccg gggatctgac gcgcatcttc
     5221 cagccgttct tcaccaccaa gcaccgcggc tccggcctcg gcctgccgat cgtgaagaag
     5281 atcatcgacg cgcacggcgg cacgatcgaa gtttcgagca aacccgggca gggaacggtg
     5341 gcgacgatca cgctgccggg ctccggcgag gtcgaatgat ggcacacgag aacatcctga
     5401 tcgtcgaaga cgaaaagctg atccgctggt cgatcaagtc gcggctggag gacgagggct
     5461 acacggtcgc ggaagtcgat acggggaaaa aggcgttcga cctgtgggac cagggggact
     5521 tcgatctgat gctgctcgat tttcgcttgc ccgacaccac cggtgtcgag atcctgcgac
     5581 gggtcaagca gcagtcgccc gagacctcgg tcgtgatgat gacggcctac ggcacggtcg
     5641 agagcgccgt cgaggcgatg aagctcggag cgttcgatta cttgacgaag ccggtgaacc
     5701 tcgaggagct ggtggtgatc gtcgagaagg cgctcgagac gaccagcctg cgccgcgagg
     5761 tgcggcggct gcgcaacgag caccgcgaga tccacggcaa cgtccaactg atcggccgca
     5821 gccgggcgat gctcgagatc atcgagctgg tggagaaggt ctgcgccagc caggcgacga
     5881 ccgtcctgct cgagggcgac agcgggaccg ggaagaacgt cgtggccaag gcgatccact
     5941 acggcagccc gcgggtcgac aagccgttcg tgacaattac ctgttcggcg ctgaccgaga
     6001 cgctgctcga gagcgagctg ttcggccacg agcgcggagc gttcaccgac gccaagaccc
     6061 agaagaaggg gttgctcgag gtggccgacg gcggcaccgc gttcctcgac gagatcggtg
     6121 agatgggcat ggcgatgcag gccaagctgt tgcgctttct cgaggagaag acgttcaagc
     6181 gggtcggcgg aacgcgcgat atcgaggtcg aggtacgcat catcgcggcg accaatcgcg
     6241 acctgcacga ggcggtccgc gaggggaggt tccgcgagga tctgtactac cgcctgaagg
     6301 tgatcccgat ccggctgccc agcttgcgcg aacgtcgtga cgacatcccg ttgctggtcc
     6361 agcattttct cgatcacttc aaccaggagt tccgcaagaa cacgacgcgc gtcgaacccg
     6421 ccgcgatgga gcggctcaag gcctacgact ggccgggcaa catccgcgag ctgcgcaatt
     6481 tcatcgagcg cgtgatgatc ttggagagca aggaatgcat cgagctcgcc gacctgcctc
     6541 gcgcgatgcg cgacgggctg tcggccgtcg agccgacccc cgccgccgac tcggacccga
     6601 gcccgatctc gctcggggcg atgacgctcg aagagatgga gcgcgccgcg atcgtgcagg
     6661 cgctcgagca ggtcagccag aatcaggtac gcgcggcgaa gctgctcggc atcacgcgag
     6721 acaccctgcg ctaccgcatg aagaagttcg gtctgctcga cggtctgcgc gagtgagctg
     6781 cgccgagcgc gctcaagcct cgagccgcag atcgcgatag tcgtccagct cgtgcgaggc
     6841 cttgagccgg cgaatcgcgg cggcctggat ctgccggacc cgctcgcgcg agacgcgcaa
     6901 gatccgaccg atctcgtcca gcgtgtacgg ccggtcgtcg ccgattccga agcgcagacg
     6961 cagcacctgc tcctcacgag gcggcaacga ctgcagcgtc tgctcgacgc gcgcgctcat
     7021 gtcgcggtgg gccaactgga cccacggcga caccgcggtg gtatcctcga cggtgtcgac
     7081 cggcgacgga ccgctctcct cgccgcgctc gtcctccagc gcgaccggct cggcgatcat
     7141 gcgcagcacc ttctccaccg cttcgagcga cagactgagc ttgcgcgcga tctcgatcgg
     7201 cagcgggtcg cggcccagcg tctggccgag cagcgtcgcc gtctgcgtga ctttgcgccg
     7261 gcggtcgtgc aggtggaccg gcaggcggat cagccgggac ttgtcggcca gtgcccggac
     7321 gatcgactgc ttgatccacc agtgggcgta ggtcgacagc ttgttgccgc gggagtactc
     7381 gaacttctcg atcgccttca tcagaccgat gttgccctcc tggatcaggt ccagcagacc
     7441 gacgccgtgg ccggcgtagc gtttggccag gtggacgacg agacgcaggt tggccaggat
     7501 cagctcttcc ttggccgact cgagttggtg gcggagacga cgcgcccgcg cgacccgttc
     7561 ggcgtccgcc tcgcgcggac gctcgcgcgc ccagcgctcg tagccgtggt ggacccgttc
     7621 ggctcgctcg agtggccagc tggcgtgggc gccgtcggcg tcgtgcggtt ggctggcacg
     7681 ccgcgctcga tagcgggccg gtagctcggc gccgagagcg atcagcccgg tcttggcccg
     7741 gtcgagcgcg gcgaacaact cccgctcgcg cctggcgtcg agcaacggga tcgcgctcat
     7801 gtcgcgcagg tagaccggga gcgcatcgtg cccgccgggg gcggccgcgt cagtctgccg
     7861 ccgcggcgca cccggcggta tcgggaaggt aacgaccgag cttttcacgt gaccgccttt
     7921 ccgttcatat acgctgaatg actgcgatcg ttgaggacag caacccgcat gccagaaaac
     7981 ggcgtcgctt gcgggccgtt tcggtcccaa ttcccgccga tcggcggcca tcacgaccgg
     8041 acgagcgccg gccgcgccag ccttggcgcg aattgaccgt cgagacccgc tcgattgagg
     8101 taaatcccgc agaccgaccg gcggaaaacc ccacctaggc caacgcggtg gcgacggcgc
     8161 gggtcatctc gtcgaggtcg aacggctttt gcaggacgtc ggagacaccg tgggccagcg
     8221 cctcccgcac cagctccggc gtccactggg cggtcatcat gatcacgcgg cagccgggac
     8281 gtcgcctgag gatctcgtcg cacaactcga gcccgccgac gtcgggcaag cgcaaatcga
     8341 gcaacaccag cgccacgcgc tcgtccagcg tcttcatcgt ctcgcgaccg gagtcggcga
     8401 gcacgacgtc gtaaccggcg tgccgaagat gctcgcgcag cgaccagcgg atcagcttct
     8461 cgtcgtcgac gaccagaatc gatgtgggga tgacccgcat gacgatgcaa ctcccgtcgt
     8521 gatcgctgga gcggcagtct gcaaatgtcg tacccgactc acggcaacag ccgtcgctcg
     8581 cgggcggcgt tttccagatc ctgctggaat cgcgggtcgg cgataccgat caacgcctcg
     8641 acgcgctcgc gcaggttctt gccgaacagg ttggtcaccc cgtgctcggt gacgacgtag
     8701 tgcacgtcgg cccggctggt cacgacaccg gcgccctctt tcagaaacgg cacgatccgc
     8761 gaggcttcgc cccgttgggc ggtcgacggg atggcgatga tcggtttgcc gcccttggaa
     8821 cgtgccgcgc cccggatgaa atcgacctgg ccgccgaagc cggaatagat ccgcgggccg
     8881 atcgaatcgg agcagacctg gccggtcagg tcgatctcga tcgcagaatt gacggcgacc
     8941 aagttgtcgt tctgtccgac gacgaacgga tcgttgacat gttcaacggg gtgcgcctcg
     9001 atgaacgggt tgttgtcgag aaagtcatac agctcgcgac tgcccagcgc gaaggtgatg
     9061 acggccttgc cggggtgcag cgtcttgcgg ttgccggtga ccacgccgcg ttcgatcgcg
     9121 cgcatcgccc cgtccgagat catctcggta tggatgccca gatcgagcgg cccgtcgatc
     9181 gcggcgtaga ccgcgtcggg aatgccgccg atccccatct ggatcgtcga tccggcctcg
     9241 atcagctcca ccacgttcct ggcgatcgcg atctcgacgg cggtcgccgg cttggcgatc
     9301 agcgtcggca gctgctcgtt ccgctcgacg atcgcgtcga cgcggctgac gtggacgaac
     9361 gaatcgccga gcacgcgcgg catctgctcg ttgacctgag cgatgacggt cttggccgcg
     9421 cggcacgccg tcttcgacgc cagtgtctcc actccgaggc tcatgaagcc gtgctcgtcg
     9481 ggaggcgaca gcatcaccat cgccacgtcg agctcgaccc ggccttcgag aaagatgcgg
     9541 gggatctggt gcaggaagat cggcaagtag tcggcgcggc cctcgttgac cgccgcgcgg
     9601 tcggccgggc ccacgaacag cgagttgtga cggaaatggc cctccatccc gggcgaggac
     9661 aacgggtcgt cgccgagcag cagcacgtga tgcagcgtca cgtcccgcag ctcgtcgcgc
     9721 cgctcggcca gcgcccgcac gagcgccgaa ggaatcgccg cgttgccgcc gtagtagacg
     9781 ttgctgtggc tggtgatgcg agcgacggcc tcggccggtt gtctgagctt ggccttgtag
     9841 tcgtcgagcc agttcatcg
//
LOCUS       DASPGN010000052         4937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_596107, whole genome shotgun sequence.
ACCESSION   DASPGN010000052 DASPGN010000000
VERSION     DASPGN010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596107"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..1037)
                     /locus_tag="VD788_01580"
     CDS             complement(324..1037)
                     /locus_tag="VD788_01580"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXV74981.1"
                     /translation="MSEQDQSFRLDDKLAAEDRLYDEMFHSRDRGIIWKALAAAIVIH
                     VAVLIINFPTFKRAIVPQRAENVIIVKKYVPPPPKVERRQVVKKQLTKKVPIPDPTPD
                     EPEPIREPEPEIEPEPVPPDVEFLIGVPEPPPESGPLIAGAGDVTNPELIAESKLEPE
                     YPELARVARLEGNVILQAIIKSDGTVGDVEVLRTNRPGMGFEDAAIKAVRQWRYKPAL
                     QNGKPVEVYFTVFVDFKLH"
     gene            complement(1117..1590)
                     /locus_tag="VD788_01585"
     CDS             complement(1117..1590)
                     /locus_tag="VD788_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV74982.1"
                     /translation="MKLKRKPENAEIPTASMADIAFLLIIFFMVTAVFSATKGLEFKL
                     PQDEQNQPPEEEEATFIKVEADSSLTVDCTPMELEEILDYLEERLRRNPEKPVILYTD
                     AYAPYQAMISVYDVLGSAEKTRGFKVKNISVPTQSEVAEYIELFGFNPFESQCAG"
     gene            complement(1603..2001)
                     /locus_tag="VD788_01590"
     CDS             complement(1603..2001)
                     /locus_tag="VD788_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV74983.1"
                     /translation="MIPTASMADIAFLLIIFFMVTTVHEVDRTSVQLPVAKIREEAEK
                     GSAIVVVHKNKEGDIAYKFSDGEVMSQAVGGPEDIYLEASRLTYVDKTKQFILKADGD
                     IHFEKVDELLDQMRRGGVQKVLLLTQQKTD"
     gene            complement(2031..2711)
                     /locus_tag="VD788_01595"
     CDS             complement(2031..2711)
                     /locus_tag="VD788_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV74984.1"
                     /translation="MDTLQNLPGDLLKYYLDGGAVMHMISVLSLFSLTTIIYKLIVFR
                     RVKTNVNEFIAKVRSNLLKGNMKGAIQACEEQRGPVAAIIKTGLLKYNDNSPREQIEK
                     TMESASIHEVAYLEKYLTVLATITNIAPLLGFLGTVVGMILSFEVIAEAGLNDPGRVA
                     KGISVALLTTAYGLIVAFVTQPFYNFFTSKVATYTREIETSVNILFETFDEMDRMSGQ
                     KSAVSPVR"
     gene            complement(2955..4526)
                     /locus_tag="VD788_01600"
     CDS             complement(2955..4526)
                     /locus_tag="VD788_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV74985.1"
                     /translation="MDKRVVLSLVLSAALGVGATAAQEPGTIELSLDETLRMALENNL
                     ELVSARYAPAFAEQDVRVQRSNFDAAFEAFYDRSESSNAVTSLATVTGDKQDMLSLGI
                     QHNLTMGANYTVGFQTLHQEQTGPQVVAPGAYFSGVAMSLTMPILKGFGTTVTTETLV
                     LAQQNHEISMTDLEAQAETVIQTVEGAYWDVVAAREALRIERLALARAQDLLELNRKK
                     VEVGTLAPIEITQAEAGVASQEEGVIVAETTLQDAQDELLRLMAIPDGDPLWETQIQN
                     STRPVFDEREVDVDAAIELALANRAEVAAAEQTLVSRELSQRVARRQVRHQLDFSANY
                     TPQGSSLDAQALIDPITGNLLIPEQNAELEESIARIYNGDLFNWNARLTYRVPIGNRA
                     AKAQYARAQLATDQAGVDLLNQEQTVRVEVRRAARGVTSGIKRVEAARKNVELQQKKL
                     DAEQKKFENGMSTSFEVLTFQNDLADAELGEIRARLDYIKSVAALERSQGTLLEARGL
                     RLDVEPASSPDRSQP"
     gene            complement(4613..>4937)
                     /locus_tag="VD788_01605"
     CDS             complement(4613..>4937)
                     /locus_tag="VD788_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HXV74986.1"
                     /translation="LRLVEHVCDRVAVMYLGRIVEHAPAAALYRRPLHPYTQALLAST
                     PSLEPGRTTSSAVRGETPSATRPPAGCPFHPRCPVAVGECATKNPRLVEAEPEHRVAC
                     HLVGQ"
BASE COUNT          779 a         1629 c         1580 g          949 t
ORIGIN      
        1 ggcggcctcg gcgtacttgc cggccttgaa gttgctcagg ccctgattca aggcgtcgtt
       61 ggccgcggcc ggcgggacga tcgcgactgc gaacagcacc ccgacgacgg tgagcttgac
      121 gattgccttc gtgctgaaca tcgctggtcc tccccggact tctgttcgtt gcggcgcggg
      181 actcggcccg cccactgacg accctcgcaa cttgcgggcc aggtcgaagc ggggcggccg
      241 accccgcgga aatcccttct accgacagcc taccagacgg gccgcgcgcg gcccgtcggc
      301 accgatgtgc catttcggca cgatcagtgg agtttgaagt cgacgaagac ggtgaagtag
      361 acctcgaccg gcttgccgtt ctgcagcgcc ggcttgtagc gccactgccg gacggccttg
      421 atcgcggcgt cctcgaaccc catgccgggc cggttggtgc gcagcacttc gacgtcgccg
      481 accgtcccgt ccgacttgat gatcgcctgc aggatcacgt tcccttcgag tcgcgccacg
      541 cgggccagct cgggatactc gggctcgagc ttgctctcgg cgatcagctc ggggttggtc
      601 acgtcgccgg caccggcgat cagcgggccc gactccggcg gcggctccgg aacgccgatc
      661 agaaactcga cgtccggcgg tacgggctcg ggctcgatct cgggctcggg ctcgcgaatc
      721 ggctcgggct cgtccggcgt cgggtcgggg atcggcacct tcttcgtcag ttgcttcttg
      781 acgacctgcc ggcgctcgac cttcggcggc ggcggcacgt acttcttcac gatgatgacg
      841 ttctcggccc tctggggcac gatcgcccgc ttgaacgtcg gaaaattgat gatcagaacc
      901 gcgacgtgga tcacgatcgc ggcggccagg gccttccaga tgatgccccg gtcgcgcgag
      961 tggaacatct cgtcgtagag ccggtcctcg gcggccagct tgtcgtccag ccggaacgac
     1021 tggtcctgtt cgctcatcgt cgccctcccg catgccccgc tcgaccgctg tccgtcatga
     1081 tacccgccgt ccggccacgg cgcccgccgg tcacgatcag cccgcgcact gggactcgaa
     1141 cgggttgaag ccgaagagct cgatgtactc ggcgacttcg ctctgcgtcg gcacggagat
     1201 gttcttgacc ttgaacccgc gggtcttctc ggccgagccg agcacgtcgt aaacggagat
     1261 catcgcctgg tacggggcgt aggcgtcggt gtagaggatc accggctttt ccgggttgcg
     1321 ccgcaggcgt tcctcgaggt agtcgaggat ctcctcgagc tccatcgggg tgcagtcgac
     1381 ggtgagcgac gagtcggcct cgaccttgat gaaggtcgct tcctcttctt cgggcggctg
     1441 gttctgctcg tcctgcggca gcttgaactc gagacccttg gtcgcggaaa acaccgcggt
     1501 caccatgaag aagatgatca gcaggaacgc gatatcggcc atcgacgccg tcgggatctc
     1561 ggcattctcc ggcttgcgtt tgagcttcat cgcgggtgcc ccctagtcgg tcttctgctg
     1621 cgtcagcagc agcaccttct gcacgccccc gcggcgcatc tgatcgagca gctcgtcgac
     1681 cttctcgaag tggatgtcgc cgtcggcctt gaggatgaac tgcttggtct tgtcgacgta
     1741 ggtcagccgc gaagcctcga ggtagatgtc ctcgggaccg ccgacggcct ggctcatcac
     1801 ttcgccgtcg gagaacttgt aggcgatgtc tccttccttg ttcttgtgga cgacgacgat
     1861 cgccgagccc ttttccgcct cctcgcggat cttggccacc ggcagctgaa cgctggttcg
     1921 gtcgacctcg tggaccgtgg tcaccatgaa gaagatgatc aacaggaacg cgatgtcggc
     1981 catcgaggcc gtcgggatca atgcttccgc cgctcgtgag cgccggacct tcatcgcacc
     2041 gggctcaccg cggacttctg gccgctcatg cggtccatct cgtcgaaggt ctcgaacagg
     2101 atgttgaccg aggtctcgat ctcgcgcgtg taggtcgcga ccttgctggt gaagaagttg
     2161 tagaacggct gggtgacgaa cgcgacgatc aggccgtagg ccgtggtcag cagcgcgacc
     2221 gagatgccct tcgccacgcg gccggggtcg ttcagccctg cctccgcgat cacctcgaac
     2281 gacaggatca tcccgacgac ggtgccgagg aagcccaaca gcggcgcgat gttggtgatc
     2341 gtcgccagca cggtcaggta cttctcgagg taggccacct cgtggatcga tgccgactcc
     2401 atcgtttttt cgatctgttc tcgcggcgag ttgtcgttgt acttcagcaa cccggtcttg
     2461 atgatcgcgg ccaccggccc gcgctgttcc tcgcaggcct ggatcgcgcc cttcatgttg
     2521 cctttgagca ggttcgatcg aaccttggcg atgaactcgt tgacgttggt cttgacccgg
     2581 cggaaaacga tcagcttgta aatgatcgtg gtcaacgaaa acaacgacag gacggagatc
     2641 atgtgcatca ccgccccgcc gtcgaggtaa tatttcagca gatcgccggg caagttttgt
     2701 agggtatcca cgaccgctcc tccgaatccc agggctcagc gccagatgag ccggcctgat
     2761 cggcagctca ccggcatcgc atctccaaac atcaatgtcg acaacaattt agaccacgta
     2821 cgctcgtcga aacgctagcc atgctacgtc agcccccata cactgtcaag agaatggaag
     2881 ccggggcgcg gagcaaccgc agcagcggtt ccgcccgttt cttgcggctt ttttgcgcgg
     2941 ggatcggccg cgggctacgg ctgcgagcga tcgggcgacg acgccggctc gacgtcgagt
     3001 cgaaggccgc gcgcttcgag cagcgtgccc tgtgaccgct cgagggcggc caccgacttg
     3061 atgtagtcga gtcgcgcgcg gatctcgccc agctcggcgt cggccaggtc gttctggaag
     3121 gtcaacacct cgaacgacgt gctcatgccg ttctcgaatt tcttctgctc ggcgtcgagc
     3181 ttcttctgtt gcagctcgac gttcttgcgc gcggcctcga cgcgtttgat gccggaggtc
     3241 acgccgcggg cggcccgacg aacctcgacg cgcaccgtct gctcctggtt caacaggtcg
     3301 accccggcct gatcggtcgc gagctgcgcg cgcgcgtact gcgccttggc cgcgcggttg
     3361 ccgatcggga cacgataggt cagccgcgcg ttccagttga acaggtcgcc gttgtagatc
     3421 cgcgcgatcg actcctcgag ctcggcgttc tgctccggta tcaacaggtt gccggtgatc
     3481 gggtcgatca gcgcctgcgc gtcgaggctc gagccctgcg gcgtgtagtt ggcggagaaa
     3541 tcgagctggt ggcgcacctg gcgccgggcg acgcgctggc tcagctcgcg gctcacgagc
     3601 gtctgctcgg ccgcggccac ctcggcgcga ttggccagcg cgagctcgat cgccgcgtcg
     3661 acgtcgacct cgcgttcgtc gaacaccggt cgcgtcgagt tctggatctg agtttcccac
     3721 agcggatcgc catcggggat cgccatcaat cgcagcaact cgtcctgagc gtcttgcagc
     3781 gtggtctcgg cgacgatcac accttcttcc tgcgaggcaa caccggcctc ggcctgggtg
     3841 atctcgatcg gcgccagcgt gccgacctcg accttcttcc ggttgagctc gagcaggtcc
     3901 tgtgcgcgcg cgagcgccaa ccgctcgatg cgcagcgctt cgcgtgcggc gacgacgtcc
     3961 cagtaggcgc cttcgacggt ctggatcacg gtctcggcct gcgcctcgag atcggtcatg
     4021 ctgatctcgt ggttctgctg ggccagcacc agtgtttcgg tggtgacggt ggtgccgaag
     4081 cccttgagga tcggcatcgt caggctcatc gccacgccgc tgaaatacgc gcccggcgcg
     4141 acgacctggg ggccggtctg ttcttgatgc aacgtttgaa agccgaccgt gtagttcgcg
     4201 cccatcgtca ggttgtgctg gatgccgaga ctgagcatgt cctgcttgtc acccgtcacc
     4261 gtcgccaagc tcgtcacggc gttcgagctc tcggagcgat cgtagaacgc ctcgaacgcc
     4321 gcgtcgaagt tggaccgttg gacgcgcaca tcctgctcgg cgaacgccgg cgcgtagcgc
     4381 gccgagacca gttcgaggtt gttctcgagc gccatgcgca gggtctcgtc gagcgacagc
     4441 tcgatcgtcc cgggctcctg cgccgcggtc gcaccgactc ccaacgccgc cgacagaacc
     4501 aggctcaaca cgactcgctt gtccatgacc cccctccggg accggtctcc gacctgaatt
     4561 ccgactgcgg cggtcgattc gccaatacta cgcgcaccaa cctccaaagg tttcattgcc
     4621 cgacgaggtg acaggctacc cgatgttcgg gctccgcttc taccagtcgc gggtttttcg
     4681 tagcacattc accgacggcg acgggacagc gcggatgaaa cgggcacccg gcaggcggac
     4741 gggtggccga cggcgtctcg ccgcgcacgg ccgacgacgt cgtgcgtccc ggctcgagcg
     4801 acggcgtcga ggccagcagg gcctgtgtgt acggatggag cggacgtcgg tagagcgccg
     4861 cagccggggc gtgctcgacg atccgaccca gatacatcac cgcgacgcgg tcacagacgt
     4921 gctcgaccag acgcagg
//
LOCUS       DASPGN010000053         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_10958, whole genome shotgun sequence.
ACCESSION   DASPGN010000053 DASPGN010000000
VERSION     DASPGN010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10958"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..925)
                     /locus_tag="VD788_01610"
     CDS             complement(236..925)
                     /locus_tag="VD788_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXV74987.1"
                     /translation="MRKVPVTPLVLVLLAVVCSTARLSPALAADSERAERWQFQIPIT
                     FTSSETFDGEADSFLEVSDDVGWGFGFGYHANERLYVGFDLTFIQASYDASVEFDLDN
                     DMVSDGTVTLGGTLDASAAQFAGQYNFLDNTITPFVRGNFGWTYIDSNIPSGPTEGVC
                     WWDPWWGYVCDTWQPTYDDTSFSYGLGVGVRADVSQSFFLDLSYNQFWIDLSESTPSF
                     NGVRLNFGWMF"
     gene            complement(1008..1526)
                     /locus_tag="VD788_01615"
     CDS             complement(1008..1526)
                     /locus_tag="VD788_01615"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXV74988.1"
                     /translation="MQSKATTVAQYLAELPADRRAALSAVRKVILANLDRDFEEGMQY
                     GMIGYYVPHRVFPAGYHCDPRQPVPFAALASQKNHMSLYLMAAYGEGLDEAWLRKAWA
                     ETGKKLDMGKCCIRFKKVEDLALDVVAEAIRRVPARAYVAQYESALRTAKKSPTKKGA
                     PKRGRRRSPSLA"
     gene            complement(1529..2842)
                     /locus_tag="VD788_01620"
     CDS             complement(1529..2842)
                     /locus_tag="VD788_01620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXV74989.1"
                     /translation="MPVTDPDVVVIGGGPAGSTVSTLLARSGYRVQLFERECFPRFHI
                     GESLIPETYWVLKRLGMLDKMKQSRFVKKYSVQFVSANGKQSAPFYFWDNKPHECSQT
                     WQVVRSEFDLMMLQHAREHGVDVHEGVRVTDVRFEGDRATGVEVRGPGGSDREVRARV
                     VVDASGQTGVLQKKLGLRVWDPVLDKGAIWTYWQGAYRDSGRDEGATVVLQTEDRRGW
                     FWYIPLHDDRVSVGVVAPFDELFRGRTSHEETYQRELDRCPAARERVARAHRVTGYFA
                     TRDYSYRATRVAGEGWVLIGDAFGFLDPLYSSGVLLALRSGEMAAEAIAGGLASGDLS
                     AARLGSWGPRFNRGVDRMRRLVCEYYDGFSFGKFVRAHPQLRGTVTDLLIGDLFEDRV
                     DGVFRAIEGMYPEGKQPPPPWNHAVSDLDVENKSHELVLPDGPRP"
     gene            2990..3370
                     /locus_tag="VD788_01625"
     CDS             2990..3370
                     /locus_tag="VD788_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74990.1"
                     /translation="MIGTRRLAALCLTLLVVGCAKRRSIEYEMHWSDLVEPDHIVLIF
                     AEFPNHYLDLVSGDLRAYLDTLPDDRVRVELELTTRLGCVERIRLVRIDDWRGAAKEW
                     AGSGWVEREDPSPWDALGCRVPWW"
     gene            3445..>3902
                     /locus_tag="VD788_01630"
     CDS             3445..>3902
                     /locus_tag="VD788_01630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV74991.1"
                     /translation="MPLRPGETLLHYRLVEQIGEGGMGLVWRAEDTTLDRQVAIKILP
                     ERFAADPSRLSRFEREAKALAALNDPGIASIYSVHLDAEVPFLAMELVEGEALRSRIG
                     PAGMPLDELLELAISLADAVAAAHAAGIVHRDLKPDNVMRTARRRVKVLD"
BASE COUNT          623 a         1334 c         1315 g          630 t
ORIGIN      
        1 cttacatcga gcactggaac acgaacccga cccccttcgt gtggaccaaa tgcccggcgg
       61 acatcatccg caaggccgtg cgccgttaac atgatttcgg ttacgcgaca ctaggtctgc
      121 cggggctcgt cgagctcgag ctggatcgag agcacgaccc cagaacgcga gccactgcga
      181 tgcaccgaag atcgggcgtc gacccaacgg atgacgaagc ctcgcatcag accgctcaga
      241 acatccagcc gaagttgagc cggactccgt tgaacgacgg tgtcgactcg ctgaggtcga
      301 tccagaactg gttgtagctc aggtcgagga agaagctctg cgacacgtcg gcgcgcacgc
      361 ccactcccag cccgtaggag aacgacgtgt cgtcgtaggt cggctgccag gtgtcgcaaa
      421 cgtagcccca ccacggatcc caccagcaga caccctcggt cggtcccgac gggatgttgg
      481 aatcgatgta ggtccacccg aagttgcccc gtacgaacgg cgtgatcgtg ttgtcgagga
      541 agttgtactg tccggcgaac tgcgccgcac tggcgtcgag cgtgccgccg agggtcaccg
      601 tcccgtccga caccatgtcg ttgtcgaggt cgaactcgac ggacgcgtcg tagctcgcct
      661 ggatgaaggt caggtcgaac ccgacgtaca gccgctcgtt ggcgtgatag ccgaagccga
      721 atccccagcc gacgtcgtcg ctcacctcga ggaacgagtc ggcctcgccg tcgaaggtct
      781 cggacgaggt gaaggtgatc gggatttgga actgccagcg ctcggcgcgc tcgctatcgg
      841 ccgcgagcgc gggcgacagc cgggcggtcg agcagacgac ggcgagcaag acgagaacca
      901 acggcgtcac cgggactttg cgcatggatc ctcctgattg cgggaagcgc gacgtagact
      961 ccgcggaacg atgaccccat cccagagcgc cgacagtata gccggtccta cgccaacgac
     1021 ggcgaccggc gcctcccgcg cttcggcgcg cccttcttcg tcggcgactt cttcgcggtg
     1081 cggagcgccg actcgtactg cgcgacgtac gcccgcgccg gcacccgccg gatcgcctcg
     1141 gccaccacgt cgagcgcgag gtcctcgacc ttcttgaagc ggatgcagca cttgcccatg
     1201 tcgagcttct tgccggtctc ggcccacgcc ttgcgcaacc aggcctcgtc gagaccttcg
     1261 ccgtaggccg ccatcaggta gagcgacatg tgattcttct gcgaggccag cgccgcgaac
     1321 ggcacgggtt ggcgcgggtc gcagtggtag cccgccggga agacgcggtg cggcacgtag
     1381 tagccgatca tgccgtactg catcccctcc tcgaagtccc ggtcgaggtt cgccaggatc
     1441 accttgcgca cggcggagag ggccgcccgg cggtcggcgg gcagctcggc caggtactgg
     1501 gcgacggtcg tggctttgga ttgcatggtc agggcctcgg tccatcgggc aggaccagtt
     1561 cgtgactctt gttctcgacg tcgagatcgg acacggcgtg attccacggc ggcggcggct
     1621 gcttgccctc ggggtacatt ccctcgatgg cgcggaagac gccgtccact cggtcctcga
     1681 acaggtcgcc gatcaacagg tcggtcacgg tccctcgcaa ttgcgggtgg gcgcggacga
     1741 acttgccgaa gctgaacccg tcgtagtact cgcaaaccag acgacgcatg cggtcgacgc
     1801 cgcggttgaa ccgcgggccc cagctgccga gccgggcggc cgagaggtca ccgctggcga
     1861 gacccccggc gatcgcctcg gcggccatct ctcccgagcg cagcgcgagc agcacgcccg
     1921 acgagtatag cggatcgagg aagccgaagg cgtcgccgat cagcacccaa ccttcgcccg
     1981 cgacgcgcgt cgcgcggtag gagtagtcgc gggtggcgaa gtagccggtc acgcggtgtg
     2041 cgcgtgcgac gcgctcgcgc gccgcgggac accggtcgag ctcgcgctgg taggtctcct
     2101 cgtggctggt ccgcccgcgg aacagctcgt cgaacggcgc gaccacaccg acgctgaccc
     2161 gatcgtcgtg cagcgggatg taccagaacc agccccgccg gtcctcggtc tgcaggacga
     2221 cggtcgcccc ttcgtcccgc ccgctgtcgc ggtacgcccc ctgccagtag gtccagatcg
     2281 cacccttgtc gagcaccggg tcccagacgc gcaagcccag ctttttttgc agcacgccgg
     2341 tctgaccgct cgcgtcgacg acgacccgcg cccgcacctc gcggtcgctg ccgcccggcc
     2401 cgcggacctc gacgccggtc gcgcgatcgc cctcgaaccg cacgtcggtg acgcggacgc
     2461 cttcgtgcac gtcgacgccg tgctcccgcg cgtgctgcag catcatcagg tcgaactcgc
     2521 tgcgcacgac ctgccaggtc tgcgagcatt cgtgcggctt gttgtcccag aagtagaacg
     2581 gggccgattg cttgccgttg gcgctgacga actgcacgct gtacttcttg acgaaccggc
     2641 tctgcttcat cttgtcgagc atgcccagcc gcttcagtac ccagtaggtc tcggggatca
     2701 gcgattcgcc gatgtggaat cgcgggaagc actcgcgctc gaacagctgg acgcggtaac
     2761 cgctccgagc gagcagggtc gagacggtgg agccggccgg ccccccgccg atgacgacga
     2821 catccggatc ggtgactggc atcggcgtgt tcatctccgg tagttacgcg ggagcggccg
     2881 aggggttcaa ccgcggcccg cgtccgcgtc cagtcattat gccgggctcc cgctcttccg
     2941 ccaatcgcgc gggtcgggcc cggggcgccg ggccccctat aatcgagcga tgatcgggac
     3001 acgccggctg gctgcgttgt gtctgaccct gctcgtcgtc ggctgcgcca agcggcggtc
     3061 gatcgagtac gagatgcact ggagcgatct cgtcgagccg gaccacatcg tgttgatctt
     3121 cgccgagttt ccgaatcact atctggatct ggtctccggc gatctgcgcg cctacctcga
     3181 caccctgccc gacgatcgcg tgcgcgtcga gctcgagctg acgacccgtc tgggctgcgt
     3241 cgagcggatc cggttggtcc gcatcgacga ttggcgcggg gcggcgaagg agtgggccgg
     3301 gtccggctgg gtcgagcgcg aggacccgtc cccgtgggac gcgctcggct gccgggttcc
     3361 gtggtggtga gccctccccg ccggtccgtc agcggcggcg gccctgtaca atccacgcat
     3421 gcgccgcgtg tcgcgtgacg gttgatgccc ttgcgccccg gagaaaccct gctgcactac
     3481 cggctcgtcg agcagatcgg cgagggcggc atgggactgg tctggcgcgc cgaagacacg
     3541 acgctcgacc gccaggtggc gatcaagatc ctccccgagc ggttcgccgc cgacccgtcg
     3601 cgtctgtcgc gcttcgagcg cgaggcgaag gcgctcgccg cgctgaacga tccgggcatc
     3661 gcgtcgatct actcggtgca cctcgacgcg gaggtgccat tcctggcgat ggagctggtc
     3721 gagggcgagg cgctgcggag ccgcatcggt ccagcgggca tgccgctcga cgagctgctc
     3781 gagctggcaa tctcgctggc ggacgcggtc gccgcggccc acgccgcggg gatcgtccac
     3841 cgcgacctga agccggacaa cgtgatgcgg acggcgaggc gccgggtcaa ggtgctcgac
     3901 tt
//
LOCUS       DASPGN010000054         5030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_830983, whole genome shotgun sequence.
ACCESSION   DASPGN010000054 DASPGN010000000
VERSION     DASPGN010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830983"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..296
                     /locus_tag="VD788_01635"
     CDS             57..296
                     /locus_tag="VD788_01635"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HXV74992.1"
                     /translation="MSESNQRAPAGPPSPKAFIGAGIEMAVAIVLFMYVGYRLDGWLA
                     SAPWCFVGGALVGVAVAFYGFFKRVRPAGHDDPEG"
     gene            303..716
                     /locus_tag="VD788_01640"
     CDS             303..716
                     /locus_tag="VD788_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74993.1"
                     /translation="MRGGAAGRYALGSLTTAVSAAIGMLLVVGLDPRALGWALAGWAW
                     MTLVGVGGGAWIAARHGDPGPGFLKALGTCILSRLAGAAGGAALAASHGADAVWPYLA
                     GLGTGFVPLQVFEIGWFLRRSRGWTVARGGDALGH"
     gene            700..1566
                     /gene="atpB"
                     /locus_tag="VD788_01645"
     CDS             700..1566
                     /gene="atpB"
                     /locus_tag="VD788_01645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HXV74994.1"
                     /translation="MLSGTETEAAQQAAEHAEQGFDPGSSILHHILDANEIEVFVPTL
                     KIHLPQLELFGVDVSITKHVVMMWVAALLLIGLTRVAVRQCGDPVPRGLRNAFEVLVL
                     FIRNEVARKSIGAGADRYVPYLLTTFFFILSCNLLGLMPGAATATGNISVTAALAIVA
                     FVVIQLGGIGEHGLIRHFANLVPHGLPFWLVPIMFVLELLSTFIKPFALCIRLFANMM
                     AGHVVILAFISLIFILGAMFSPVVGLAASPLAVFFALFVYVLEILVAFLQAYIFTMLT
                     ANFVGMSVHPAH"
     gene            1630..1860
                     /gene="atpE"
                     /locus_tag="VD788_01650"
     CDS             1630..1860
                     /gene="atpE"
                     /locus_tag="VD788_01650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HXV74995.1"
                     /translation="MEGLGLAYLGAGIGAGIAIIGGGIGIGLLANGALQGISRQPQAA
                     ATIQTAMLIAAALIEGIAFLALVICFLLAIKA"
     gene            1872..2468
                     /gene="atpF"
                     /locus_tag="VD788_01655"
     CDS             1872..2468
                     /gene="atpF"
                     /locus_tag="VD788_01655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HXV74996.1"
                     /translation="MRSLKRYALPLGLAAAALPAAAAEGGGSSLIEPKFGTIFWTLIT
                     FGALVFVLGRYAWKPLLGALREREGSIEESLEQARCDRQQAESLLAQQRELVEQSRRE
                     RAAAVEHGRRDAEKLKADILDEARAQRQKMLEQTQLQIEAGMRQARAELRSDTVDLAL
                     AAAKKLLVRSLDETAHRTLIEEYLAELERSAGTESRPS"
     gene            2473..3579
                     /gene="aroB"
                     /locus_tag="VD788_01660"
     CDS             2473..3579
                     /gene="aroB"
                     /locus_tag="VD788_01660"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HXV74997.1"
                     /translation="MDELVLSLRSAPRRTCRIHVGVRVLDRLVEDLAADPPGRPLVVI
                     SDETVARLHGRTFVARLTGRVAAELLTFPPGEAHKTRETKAALEDALLAREVGRDAAI
                     VAFGGGVTGDVAGFVAATWQRGVPVVQVPTSLVAMVDAALGGKTAVNLLAGKNLVGAF
                     HQPHAIWADVGLLDTLPDGELRQGLAEVVKSACIGDPRLFRWLERAVDALLGRDRAAL
                     CRVVGDCLRIKARTVARDERERGRRAVLNFGHTVGHAIEVATGYAIGHGDAVAIGMCV
                     EAELATEATGFARADARRLTRLLERFGLPTRPPADLPPEALARATRRDKKARAGRVYY
                     ALPRSIGRMIAGPRVTVEVGHEALVAALGRIGSN"
     gene            3688..>5030
                     /locus_tag="VD788_01665"
     CDS             3688..>5030
                     /locus_tag="VD788_01665"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV74998.1"
                     /translation="MKLGRYEIESELGRGAMGRVLLAHDPEIDRKVAIKIVQSLQGLA
                     GADRGEARDRFLREARSAGKLLHPGIVTVFDVGEVQGTPYLAMEYVQGRTLDAYCRPD
                     GLLPVRQAVEMVAGAAEALSYAHAAGVVHRDIKPANLMRTGESSVKIMDFGLARGAEA
                     QVTHDGALLGTPSYMSPEQIRGRAVDGRSDLFSLGVVLYELLTGVKPFPGDSVSSVIY
                     RVVHEQPHDAAEVNPRVPPALGRLLRRALDKRVDQRFQTGDELAAALRKIAATLDVAP
                     GSAGGSDSGRPAGPAPPRRPAAGRSIPPSPRPTVKRSSPLPFVLAIAAVLALLAGGSY
                     YYRKSLGFDGLFDRPPVVYQTRVTVEPAETAVTVNGEPFDPTGDGVVQFTAGGPPTTV
                     IARHACREERHELGAEQAGGELAIVFEPVRLAYELALDGVGAEVELNGEPVGRTPV"
BASE COUNT          671 a         1692 c         1838 g          829 t
ORIGIN      
        1 gaggcaggtg ccgaaacggc gcggcgttgc tacagtacga ctcttcggac gccgtcgtga
       61 gcgagtcgaa ccagcgtgcg ccagcaggtc ccccgagccc gaaggctttc atcggagcgg
      121 ggatcgagat ggcggtcgcg attgtgctat ttatgtacgt cggctaccga ctcgacggct
      181 ggctggccag cgcaccgtgg tgtttcgtcg gcggggcgct ggtgggggtg gcagtcgcgt
      241 tctacggctt tttcaagcgc gtccgaccgg ccgggcacga cgacccagag ggatagagca
      301 cgttgagagg cggggcagcg gggcggtacg cgctgggcag cctgacgacc gccgtctcgg
      361 cggcgatcgg gatgctgctc gtggtcggtc tcgacccgcg ggcgctcggt tgggcgctcg
      421 cgggctgggc ctggatgacg ctcgtcggcg tcggcggcgg cgcgtggatc gccgcacgcc
      481 acggcgaccc gggtccgggc tttctcaagg cgttgggaac gtgtatcctg tcgcgcttgg
      541 ccggcgcggc cggcggcgcg gcgctcgccg cctcccacgg cgcggacgcg gtgtggccct
      601 atctcgccgg cctcgggacg gggttcgtgc cgctgcaggt gttcgagatc ggctggttcc
      661 tcagacgatc gcggggatgg accgtggcgc ggggtggtga tgctctcggg cactgagacc
      721 gaagcggcgc agcaggccgc cgagcacgcg gagcagggct tcgacccggg ctcgtcgatc
      781 ctgcaccaca tcctcgacgc caacgagatc gaggtcttcg ttccgacgct caagatccac
      841 ctgccgcagc tcgagctgtt cggcgtcgac gtctcgatca ccaagcacgt cgtgatgatg
      901 tgggtcgccg cgttgctgct gatcggtctg acgcgggtcg cggtccgcca atgcggcgac
      961 ccggttccgc gcgggctgcg caacgcgttc gaggtgctcg tcctgttcat ccgcaacgag
     1021 gtcgcgcgca agtcgatcgg ggccggtgcc gaccgctacg tgccgtacct gttgacgacg
     1081 ttcttcttca tcctctcgtg caacctgctc gggctgatgc ccggtgcggc gacggcgacc
     1141 ggcaacatct cggtcaccgc ggcgctggcg atcgtcgcct tcgtcgtgat ccagctcggc
     1201 ggcatcggtg agcacggtct gatccgacac ttcgccaacc tggtgccgca cggactgccg
     1261 ttctggctcg tgccgatcat gttcgtgctc gagttgctca gcaccttcat caagccgttc
     1321 gcgctctgca tccgtctgtt cgccaacatg atggccggac acgtggtgat cctggccttc
     1381 atttctttga tcttcatcct cggcgcgatg ttctcgccgg tcgtcggctt ggccgcttca
     1441 ccgctggccg tcttcttcgc gctgttcgtc tacgtgctcg agatcctggt ggccttcctc
     1501 caggcgtaca tcttcaccat gttgaccgcc aacttcgtcg gaatgtcggt acatcccgca
     1561 cactgatcga atcgattcga gcgaaccgcg cgacaggtcg agagagagca acaaaggagg
     1621 accggattca tggaaggact aggtcttgcg tatctgggcg cgggcatcgg cgccgggatc
     1681 gccatcatcg gtggcgggat cgggatcggc ctgttggcca acggtgcgct gcagggcatc
     1741 tcgcggcagc cgcaggcggc ggccacgatc cagacggcaa tgctgatcgc ggcggcgctg
     1801 atcgagggca tcgcgttcct ggcgctggtg atctgcttcc tgctcgcgat caaggcctga
     1861 cgggtcggcg tgtgcgttcg ctgaaacgat acgcgctgcc gttgggcctg gctgccgcag
     1921 ccctgccggc cgcggcggcc gagggcggtg gttcgtcgct gatcgagccc aagttcggca
     1981 ccattttctg gacgctgatc accttcggcg ccctcgtctt cgtcctcggc cgttatgcct
     2041 ggaagccgct gttgggcgcg ctgcgcgagc gcgaggggtc gatcgaggaa agcctcgagc
     2101 aggctcgttg tgaccgccag caggcggagt cgctgctcgc acagcagcgg gagctggtcg
     2161 agcagtcgcg acgcgaacgc gcggcggccg tcgagcacgg gcggcgcgat gccgagaagc
     2221 tcaaggccga catcctcgac gaggcgcgcg cgcagcgcca gaagatgctc gagcagaccc
     2281 agttgcagat cgaggcgggt atgcgacagg cacgtgccga gctgcgatcc gacacggtcg
     2341 acctcgcgct cgccgcggcg aagaagctcc tggtccgatc gctcgacgag acggcccacc
     2401 gcacgctgat cgaagagtac ctggccgagt tggagcggtc cgcgggcacg gagtcgcgac
     2461 cttcctaggg ctgtggacga actggtcctt tcccttcgct cggcgccacg ccgcacctgc
     2521 cgaatccacg tcggcgtgcg ggtcctcgat cgtctggtcg aggatctcgc cgccgacccg
     2581 ccggggcggc cgctcgtcgt catctcggac gagacggtcg cgcggctgca cggccggacg
     2641 ttcgtcgcgc ggctgaccgg gcgggtcgcc gcggagctct tgacctttcc ccctggggag
     2701 gcgcacaaga cgcgcgagac gaaggccgcg ctcgaggacg cgctgctcgc gcgcgaggtc
     2761 gggcgcgacg cggcgatcgt cgccttcggc ggcggcgtga ccggggacgt ggccggcttc
     2821 gtcgcggcga cctggcagcg cggcgttccc gtcgtccagg tgccgacctc gctcgtcgcg
     2881 atggtcgacg ccgcgctcgg cggcaagacc gcggtcaacc tcctcgccgg caagaacctg
     2941 gtcggggcgt tccaccaacc gcacgcgatc tgggccgacg tcgggttgct cgacacgctg
     3001 ccggacggag agctccgcca aggattggcc gaggtcgtca agtcggcctg catcggtgac
     3061 ccgcggctgt tccgctggct cgagcgtgcg gtcgacgcgc tgctcgggcg ggaccgcgcc
     3121 gcgctctgtc gcgtcgtcgg cgattgcctg cggatcaagg cgcggaccgt ggcgcgggac
     3181 gagcgcgagc gaggccgccg ggccgtgttg aacttcgggc acaccgtcgg acacgcgatc
     3241 gaggtcgcga cgggctacgc gatcggtcac ggcgacgcgg tcgcgatcgg catgtgcgtc
     3301 gaggccgagt tggcgaccga ggcgaccggg tttgcgcgcg ccgacgcgcg ccggctgacc
     3361 cggctgctcg agcggttcgg cctgccgacg cgcccgccgg ccgacctccc gcccgaggcg
     3421 ctcgcccgag ccacccgccg cgacaagaaa gcgcgcgccg ggcgggtgta ctacgcgctg
     3481 ccgcggtcga tcggccggat gatcgccggg ccgcgggtca cggtcgaggt cgggcacgaa
     3541 gcgttggtcg cggcgctggg gcggatcggc tcgaattgac ttatcccgag gggcgggtta
     3601 gctttttgaa cgtcgcgtcc cgggtgtggt gatgccccgt ctttcggcaa tccgccacac
     3661 cgcgatcgtg ccccggcgaa gcaacccgtg aaactcggac gttacgagat agagagcgag
     3721 ctcggacgtg gcgcgatggg gcgtgtgctg ctggcgcacg acccggagat cgaccgcaag
     3781 gtggcgatca agatcgtcca gagcctgcag ggtctggcgg gggcggaccg cggcgaggcg
     3841 cgcgatcggt tcctgcgcga ggcgcgctcg gccggcaagt tgctgcatcc cggcatcgtc
     3901 acggtcttcg acgtcggcga ggtccaaggc actccgtacc tcgcgatgga gtacgttcag
     3961 ggtcgcacgc tcgacgccta ctgccggccg gacggcctgc tgccggtgcg gcaggcggtc
     4021 gagatggtcg ccggcgcggc cgaggcgctg tcgtacgcgc acgccgcggg tgtcgtccat
     4081 cgcgacatca agccggccaa cctgatgcgg accggcgagt cgtcggtcaa gatcatggat
     4141 ttcggtctgg cgcgcggcgc cgaggcccaa gtgacgcacg acggcgccct gctcggcacg
     4201 ccgagctaca tgtcgcccga gcagatccgc gggcgcgccg tcgatgggcg cagcgacctg
     4261 ttctcgctcg gggtcgtgct gtacgagctg ctgaccggcg tcaagccctt tcccggcgat
     4321 tccgtgtcgt cggtcatcta ccgcgtcgtc cacgagcagc cgcacgatgc ggcggaggtc
     4381 aacccgcggg tgccgccggc gctcgggcgg ctactgcgcc gtgcgctcga caagcgcgtc
     4441 gatcagcggt tccagaccgg cgacgagctc gccgccgcgc tgcgcaagat cgcggcgacg
     4501 ctcgacgtcg cgccggggtc ggccggagga tccgactccg gccgaccagc cggtccagcg
     4561 ccaccccgcc ggccggcggc ggggcgttcg atcccgccgt cgccgcgccc gacggtcaag
     4621 cgcagctcgc cgctgccgtt cgtgctggcg atcgcggcgg tgctggcctt gctcgccggc
     4681 ggttcttact actaccgcaa gtcgctcggg ttcgacggtc tgttcgatcg tcctccggtc
     4741 gtctaccaga cgcgcgtgac cgtcgagccg gccgagacgg ccgtgaccgt caacggcgag
     4801 ccgttcgacc ccaccggtga cggcgtggtc cagttcaccg ccggcgggcc gccgacgacc
     4861 gtgatcgcgc gccacgcgtg ccgcgaggag cgtcacgagc tgggtgccga gcaggcgggc
     4921 ggcgagctcg cgatcgtgtt cgagccggtc cggctggcct acgagctcgc gctggacggc
     4981 gtcggggcgg aggtcgagct caacggcgag ccggtcggcc ggaccccggt
//
LOCUS       DASPGN010000055         4152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_479603, whole genome shotgun sequence.
ACCESSION   DASPGN010000055 DASPGN010000000
VERSION     DASPGN010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479603"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VD788_01670"
     CDS             <1..1000
                     /locus_tag="VD788_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV74999.1"
                     /translation="DEAGNFEGRSIPNLLGGTLEQRAGEIGMPLDQLVERLGRLHDRL
                     RAARERRVRPGTDDKILTAWNGLMVSAFARAYQAFGREADLDAARAALGFVASRMLDD
                     DRVRVSYRAGRAKLNGYLDDYAFLGRGALDLYEASFDDRWLTLAERLARSLSTHFADR
                     DQGGFWFVSHDHEGLIARNRSAQDGALPSGAGVAVELLLRLAAHLDDDPFRELAERAL
                     AAYRPAVDRAPSAFASLLIAADWARGPLTEVAIVGASADRGAMRELLDVVHARFRPRL
                     VLAAGSPVDGGPRRPLLAGKQPLDGRPTAFVCRDYACRQPVTTAAALAAELDRIDG"
     gene            complement(1030..2100)
                     /locus_tag="VD788_01675"
     CDS             complement(1030..2100)
                     /locus_tag="VD788_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXV75000.1"
                     /translation="MANVLVIGRDRPSADEIRAILRQDGHDVTCTDAISGWQHVESDV
                     KPELVVAAVPSPSDVLVDDSRDAASGFPAPLLFVLEGPRSREHPYMDDRLVDRIVTPY
                     LDEELLARVDALVRVRRVVDGNALDAGVRPRPVSARLRDVGRRVVNTLTTGLSHRERP
                     LEAYLEVAARVARWADRRDAYMPGHAERVTTLCAMMADALAFDDTETGTLMRAAMLHD
                     IGKVSLPVEVLHQKSPLLDEQMRMIRTHPRKGAALIHELDPDDDVAEAILYHHERPDG
                     FGYYGTDGDRVPRAARALAVAEVYDGMTSSSALRPLPRDEALDRLHAFRGVSLDSDCV
                     EALVDRLGPTAGPGAGSIPSVG"
     gene            complement(2142..2603)
                     /locus_tag="VD788_01680"
     CDS             complement(2142..2603)
                     /locus_tag="VD788_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75001.1"
                     /translation="MTSFGRWTPRASARTRLLLAAITWTAVGAGLCWVGTAWIAVAEG
                     ALRWGLLAAAVAVGLLKARVVLAGVAGANARRIAAAGDGYCLWGYLTWRAWLFVGAMM
                     LFGATLRRLPLARPWLGSVYLAAGTALIAGSIVSWRFWSRHRPRRSPGPPA"
     gene            2703..3164
                     /locus_tag="VD788_01685"
     CDS             2703..3164
                     /locus_tag="VD788_01685"
                     /inference="COORDINATES: protein motif:HMM:NF017876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HXV75002.1"
                     /translation="MPESWSLVIALLLAVLVGASLPVLFQLFLTLGTARRTIRQLGPK
                     LSEMLSELHHTTGRLNRATSGLDHSARRASSLFEAAGELGDSIKKLNRSMRPAVMFGT
                     AIGPALAVAIKTMVDRYAAGDAAAEGATADPRPAGSTGETRPDTSKETNDE"
     gene            3157..3426
                     /locus_tag="VD788_01690"
     CDS             3157..3426
                     /locus_tag="VD788_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HXV75003.1"
                     /translation="MSDNNRYGGAHLLLAFIAGGITGAAVALLSAPQSGAESRDTLSR
                     LAKNAQGKAVRVPTALRRAYHEASEAAKKAFTESFAESASEKTEG"
     gene            complement(3380..>4152)
                     /locus_tag="VD788_01695"
     CDS             complement(3380..>4152)
                     /locus_tag="VD788_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV75004.1"
                     /translation="MRRTARQAIDAGWVVARMNLRNCGGTEPLSTTLYNAGQEQDVGC
                     VLAALDRGGAPRPLAVAGFSLGGNLVLLHAGRSADSCVADAVIAVNPPIELERCADAI
                     EMPRNRVYQAYYVRRLCTQLRRIAAVRSVPGDPPVPAEIRTVRRFDDRYTAPDAGFDS
                     AAAYYRASSAAPVLDAVRRPALIVSAEDDPFVPHEMFMRHHALPHFAFVHPAGGGHCG
                     YIQSGRPWFWAAHALLGFLDSAPVSPRSSPTRSRRTIR"
BASE COUNT          643 a         1497 c         1462 g          550 t
ORIGIN      
        1 cgacgaggcg ggcaatttcg aggggcggag catccccaac ctgctgggcg ggacgctcga
       61 gcagcgagcc ggtgagatcg gcatgcccct cgaccagttg gtcgaacgcc tcggccggct
      121 acacgaccgc ctgcgtgcag cgcgggaacg gcgcgtccgc cccggaaccg acgacaagat
      181 cctgaccgcg tggaacgggc tgatggtctc ggcgttcgcg cgggcgtacc aggcgttcgg
      241 tcgagaggcg gacctcgacg ccgcgcgagc cgcgctcggc ttcgtcgcct cgcggatgct
      301 cgacgacgat cgagtgcgcg tctcctatcg cgccggccgt gccaagctca acggctacct
      361 cgacgactac gcgttcctcg gccgcggcgc gctcgatctg tacgaggcct cgttcgacga
      421 ccgttggctg acgctggccg agcggctcgc gcgcagcctg tcgacccact ttgccgatcg
      481 cgatcagggc ggcttctggt tcgtctcgca cgaccatgag ggtctgatcg cgcgcaatcg
      541 ctcggcgcag gacggcgcgc tgccgtcggg cgccggtgtc gcggtcgagc tgctgctgcg
      601 gctcgccgcc catctcgacg acgatccgtt ccgcgagctg gcggaacggg cgctcgcggc
      661 ctaccgtccg gcggtcgatc gcgccccgtc ggcgttcgcc tcgttgctca tcgccgccga
      721 ctgggcccgc gggccgctga ccgaggtcgc gatcgtcggc gcgtccgcgg accgcggcgc
      781 aatgcgcgag ctgctcgacg tggtccatgc ccgattccgt ccgcggctcg tgctcgccgc
      841 aggatcgccg gtcgacggcg gaccgcggcg accgctgctc gcgggcaaac agccgcttga
      901 cggccgaccg accgcgttcg tctgtcgcga ctatgcctgc cggcagccgg tgacgacggc
      961 cgcggccctg gcggccgagc tcgatcgcat cgacggctag gcgggccagg ccgggagccg
     1021 agccagccgt cagcccaccg aggggatcga tccggcaccg gggcccgcgg tcggaccgag
     1081 gcggtcgacc agtgcctcga cacaatcact gtcgagactg acgccgcgga acgcgtgcag
     1141 gcggtccagg gcctcgtctc gcggcagcgg gcgcagggcg ctcgagctcg tcatgccgtc
     1201 gtagacctcg gcgaccgcca gcgcgcgcgc cgcgcgcggg acccgatcgc cgtccgtgcc
     1261 gtagtaaccg aaaccgtcgg ggcgctcgtg gtgatagaga atcgcttccg ccacgtcgtc
     1321 gtcgggatcg agctcgtgga tcaacgccgc ccccttgcgg gggtgcgtgc ggatcatccg
     1381 catctgctcg tcgagcagcg ggctcttctg gtgcagcacc tcgacaggca gcgacacctt
     1441 gccgatgtcg tgcagcatcg cggcgcgcat cagcgtgccc gtctccgtgt cgtcaaaggc
     1501 gagtgcatcg gccatcatcg cgcacagggt ggtgacgcgc tcggcatgcc ccggcatgta
     1561 cgcgtcgcgg cgatcggccc agcgcgcgac cctcgccgcg acctcgaggt atgcctcgag
     1621 cggccgctcg cggtgcgaca ggccggtggt cagggtgttg accacccgtc gtccgacatc
     1681 ccgcagccgc gcggagaccg gacggggccg gacgcctgcg tcgagcgcgt tgccgtcgac
     1741 gacccgacgg acgcggacga gcgcatcgac ccgggcgagc agttcctcgt cgagataggg
     1801 cgtgacgatc cgatcgacca gacgatcgtc catgtagggg tgctcgcgcg agcgcggccc
     1861 ctcgagcacg aacagcagcg gggccggaaa acccgaggcc gcgtcgcgcg agtcgtcgac
     1921 gaggacgtcc gacggcgacg gtacggccgc gacgaccagc tcgggcttca cgtcggactc
     1981 gacgtgctgc caccccgaga tcgcgtcggt gcacgtcacg tcgtgcccgt cctgtcgcag
     2041 gatcgcccgg atctcgtccg ccgacggacg gtcccggccg atgaccaaga cgttggccat
     2101 gaccacctcc gcaagcgaat ataggccgcg gagcccggga gtcacgccgg cgggccgggg
     2161 ctgcggcgag ggcggtgtcg gctccagaat cgccaggaaa cgattgaccc ggcgatcagc
     2221 gccgtcccgg ccgccagata gaccgacccg agccacgggc gggccagcgg cagccgcctg
     2281 agcgtggcgc cgaacagcat catggcgccg acgaacagcc acgccctcca cgtcagatag
     2341 ccccagagac agtacccgtc gcccgcggcg gcgattcgtc gagcgttggc ccccgcgacc
     2401 ccggccagta cgacacgcgc cttgagcagg ccgaccgcga ccgccgccgc gagcagcccc
     2461 caacgcagcg ctccttccgc caccgcgatc cacgcggtgc cgacccagca cagacccgca
     2521 ccgaccgcgg tccaggtgat cgccgccagc agcaaccgcg ttcgcgccga cgcccgcggc
     2581 gtccatcgcc cgaacgacgt catcgaccgg tgcctccggc ccggaaggcc cgcgatacgt
     2641 cagacgttgt tgctaatatg gagatccgcc gacggattcg ccccaacgat tcgaggtgcc
     2701 acatgcctga gtcctggtca ctcgtcatcg cgttgctgct cgcggttttg gtcggggcca
     2761 gtctacccgt cctgttccag ctctttctga ccctgggcac ggcgcggcgg acgatccggc
     2821 agctcggccc caagctgtcg gagatgctga gcgagttgca ccatacgacc ggacgactca
     2881 accgggccac ctccggactc gaccacagcg cgcgacgcgc ctcgtcgctg ttcgaggcgg
     2941 cgggcgaact cggcgattcg atcaagaagc tgaaccgatc gatgcggccg gccgtgatgt
     3001 tcgggacggc gatcggcccg gcgctggcgg tggcgatcaa aacgatggtg gaccgctacg
     3061 ccgcgggcga cgccgccgcg gagggagcga cggcggaccc acgaccggcc ggctcgaccg
     3121 gtgaaaccag acccgacacc tcaaaggaga cgaacgatga gtgacaacaa ccgctacggt
     3181 ggagcccatc tgctgctggc gttcatcgcg ggcggcatca ccggcgccgc ggtggcgctg
     3241 ttgtccgcgc cgcaaagcgg tgcggagagc cgcgacacgc tgtcccgcct ggcgaagaac
     3301 gcacagggca aggcggtccg ggtgccgacg gcgctgcgcc gggcctacca cgaggcgagc
     3361 gaggcagcca agaaggcgtt caccgaatcg ttcgccgaga gcgcgtcgga gaagaccgag
     3421 ggctgaccgg cgccgagtcg agaaagccga gtagggcgtg ggccgcccag aaccacggcc
     3481 gccccgactg gatgtaaccg cagtgacctc cgccggcggg gtggacgaac gcgaaatgcg
     3541 gcagagcgtg gtgccgcatg aacatctcgt gcggcacgaa cgggtcgtcc tcggcagaga
     3601 cgatcagcgc ggggcggcgg acggcgtcga gcacgggcgc cgcgctcgac gcccgatagt
     3661 acgccgcggc ggaatcgaat cccgcgtcgg gcgcggtata gcgatcgtcg aaccggcgaa
     3721 cggtgcggat ctccgcgggc accggcgggt cgcccgggac cgaccggacg gccgcgatcc
     3781 ggcgcagctg agtacacagc cgccgtacgt agtacgcctg gtagacccgg ttccgcggca
     3841 tctcgatcgc gtccgcgcaa cgctcgagct cgatcggcgg attgacggcg atgacggcgt
     3901 ccgcgacgca cgaatcggcc gaccggccgg cgtggagcag cacgaggttc ccgcccagcg
     3961 agaaccccgc gaccgccagc ggccgcggtg cgccaccgcg gtcgagcgct gccagcacgc
     4021 aaccgacgtc ctgctcctgg ccggcgttgt acagcgtcgt gctcaacggc tccgtcccgc
     4081 cgcaattgcg caggttcatc cgtgcgacga cccaccccgc gtcgatcgcc tgccgcgcgg
     4141 tgcgccgcat gt
//
LOCUS       DASPGN010000056         6921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1416030, whole genome shotgun sequence.
ACCESSION   DASPGN010000056 DASPGN010000000
VERSION     DASPGN010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416030"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="VD788_01700"
     CDS             <1..1284
                     /locus_tag="VD788_01700"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HXV75005.1"
                     /translation="PIRDAVESLVEMRRLLPLVELVLDGRVWPVLDDRLKTVAEIVVE
                     EGRALEPELPERRVRLDPLLRVVPVAGYDEVVRRLRSLAERAAGIRLHDGCRLERTLV
                     ALGRDPARESAKPGYRLEPNMRSDDAMKSIHRVLLDTMQRNEGGIRADIDSEFLHDFR
                     VAVRRTRSGLAQVPDVFPRAVVSGYRERFAWLGERTGPLRDLDVHRLTLRQVRRELPE
                     ELRHALDPFEAELGRRRAEEHGRLVHVLDSARYAQLVERWRGFLNKPSPRRTSLGNAM
                     RPIRAVAGERILEAWRKVIKRGHAIEPDSPARRLHRLRISCKKLRYLLEFFAELYPEA
                     ELARVIRPLKKLQNNLGEFNDLAVQRREVLDWRGAAQAAAPTADETEAAAALLLDRID
                     RDSRRTRAQFDERFAAFETARIGTLFARLFGERAG"
     gene            1281..2063
                     /locus_tag="VD788_01705"
     CDS             1281..2063
                     /locus_tag="VD788_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXV75006.1"
                     /translation="MRVLAVYSIKGGVGKTATAVNLAYLSARAGARTLIWDLDPQAAT
                     TFYFRVAPKVSGGIRKLVRGKRAIDRLIRGTDYDGLDLLPADFSYRNLDLELDATRKP
                     RSRLAKRIRPLRRRYDHLYIDCAPNISLASEGIFEAADALLVPIIPTTLSQRSLRQLL
                     GHLDQQGPKRLSVLPFFCMVDRRKSLHRQICAADGGGSVRYLAARIPYSSEVERMGAR
                     RAPLATYAPACEAAQAYEALWREVLGHTGSWLGWFRTDRFGF"
     gene            complement(2068..3027)
                     /locus_tag="VD788_01710"
     CDS             complement(2068..3027)
                     /locus_tag="VD788_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV75007.1"
                     /translation="MASFGEKLRRARESRSITLEEIADGTKIGVRYLRALEENDFEPL
                     PGGVFDRGYVRAFAQAVGLDPDATVRAFLAERRSAEPDPPEDRTIEALHLAADRNAAA
                     SSATFPRLSLPISLWTALIAVGAVAALGLGAWGIAALIDSGESGGASGRPPAPSSAPA
                     GPAVEGGQAEAAGELRETADPPSDDRPPRAVEPAADVDPAPVPEAATPPEPVPARPAP
                     VPEPPPEEPERDTAPVDAEANGGLRARIVIGRPVTGSLNCDNRRVEMLDGLRAGTELQ
                     LTCRRYLLLNVADGGAIRVGLNGAPPQPLASDGERLHGYRITP"
     gene            complement(3137..3586)
                     /locus_tag="VD788_01715"
     CDS             complement(3137..3586)
                     /locus_tag="VD788_01715"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HXV75008.1"
                     /translation="MNGMNHRCAALWLACAIGSAAFLPAAAEEGRAESHLSVPESGVG
                     SGIADRELEGEGDRFAEGTAVVFWTRVVGGQEGERIRHVWLREGQEVISIGLTLGGPQ
                     WRTYSRKTLHPGSAGNWTVEARDGEDRVIARAEFVCVGDESATEPGE"
     gene            complement(3583..6891)
                     /locus_tag="VD788_01720"
     CDS             complement(3583..6891)
                     /locus_tag="VD788_01720"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXV75009.1"
                     /translation="MRSLSRLLASLLLAGVFPGARAEPPLGYYREPAIHGETIVFAAE
                     GDLWKVGLDGGRARRLTSHPGDEGRSSISPDGRTVAFVAEYEGPSELYTMPFDGGLPT
                     RRTYGASGVSVAGWTPDGRVLFSTQSFATLPDWQLVLLDPDDGALERIPLAQAADGVF
                     DDAGTTLYFTRLPFQGSHTKRYRGGTVEQLWRWADGADEAEPLTTDYAGTSKSPMYWR
                     GRVYFVSDRDGTMNLWSMTVDGGGLRQHTTHVGWDVASPSLSDGTIVYQLGADLHAYR
                     IEAARDAVIGIRLDTDLDQTRERWVDEPMEYVTAYHPSPDGKRVVLTARGRVFVAPQR
                     QGRFVEVTRSAGVRYRDARFLPDGETLVALSDASGEVELWRLPANGIGEETRLTSDGD
                     VLRWEVVPSPDGRYLAHHDKSQRLWIYDTERRTNRLIDENRVADFRQLAWSPDSRWLA
                     YVAAADNMFSRIKIWGVASGTSRELTSDRFDSYEPVFSPDGDWLYFLSDRELSSIVGN
                     PWGNYQPEPYLDKTTRICLVALRPGLRSPFAPPDELHDDVADEEQERDDDDDEDEKKG
                     RKQAGGEREPVKIDFDGIERRVGFVPLPPGNYGSLALNDKHLFWLSRKAGESTSTLVG
                     VKISRQPLEVETLVDDVRGFEPSGDGKKLLLRKDDKLYVIDAEPKKAELDKRDVDLAG
                     WRLSIIPRDEWRQMFVEAWRLERDYFYDPGMHGVDWKAMLDKYRPLVDRVTTRAELSD
                     LIARMVSELSALHIFVSGGDMRDGPDEIAPASLGAVLERDEPAGGYRVAHIYRSDPDK
                     PQAASPLARPGVELGEGDVIDAVNGRGTLDVPDIGVLLRGQAGRQLLLGVRPAGGSKR
                     REVIAVPMTVSEAADLRYHEWEYTRRLEVERKGEGRIGYLHLRAMGGGDYTSWAEGFY
                     PVFDRQGLIIDVRHNRGGNIDSWIISRLLRKAWFHWSQRTGYAPSWNMQYAFRGHVVV
                     LCNERTASDGEAFSEGVKRLDLGTVIGTRTWGGEIWLTGSNRLVDRGIATAAEFGVYG
                     PEGEWLIEGHGVEPDIVVDNLPHATFNGEDAQLDAAIDWLEKRIAEQPVERWSPPAYP
                     DKSAGDDGDPTRKE"
BASE COUNT          979 a         2487 c         2410 g         1045 t
ORIGIN      
        1 ccgatccgag acgcggtcga atcgctggtc gagatgcgcc gcctgctgcc gctcgtcgag
       61 ctggtgctcg acggccgcgt ctggccggtg ctcgacgacc ggttgaagac cgtcgccgag
      121 atcgtcgtcg aggagggacg cgcgctcgag ccggagctgc ccgagcgcag ggtgcggctc
      181 gatccgctgc tgcgggtcgt gccggtggcc ggctacgacg aggtggtgcg gcggctgcga
      241 tcgctggccg agcgggcggc gggcatccgg ttgcacgacg gctgccggct cgagcggacg
      301 ctcgtggcgc tcggccgcga cccggcacgc gagagcgcca agccgggcta tcgcctcgag
      361 ccgaacatgc gcagcgacga cgcgatgaaa tcgatccatc gtgtcttgct cgatacgatg
      421 cagcgcaacg agggcggcat ccgcgccgat atcgacagcg agttcctcca cgacttccgc
      481 gtcgccgtgc gccgcacgcg ctcgggcctc gcccaagtgc ccgacgtctt tccccgggcc
      541 gtggtcagcg gctaccgcga gcgcttcgcc tggctcggcg agcggaccgg accgctgcgc
      601 gacctcgacg tgcaccgatt gacgttgcgc caggtgcggc gcgagctgcc cgaggagctg
      661 cggcacgcgc tcgatccgtt cgaggccgag ctggggcggc gccgcgccga ggagcacggg
      721 cggctggtcc acgtgctcga ctcggcgcgc tacgcgcagc tcgtcgagcg ctggcgcggg
      781 ttcctgaaca agccgtcgcc gcgacggacg tcgctcggga acgcgatgcg accgatccgc
      841 gcggtcgccg gcgagcgcat cctcgaggca tggcgcaagg tgatcaagcg cgggcatgcg
      901 atcgagcccg attcacccgc ccggcgactt caccggctgc gcatcagctg caagaagctg
      961 cgctacctgc tcgagttctt cgccgagctc taccccgagg ccgagctggc ccgggtgatc
     1021 cggccgctga agaagctgca gaacaacctc ggggagttca acgatctggc ggttcagcgc
     1081 cgcgaggtgc tcgactggcg gggcgcggcc caggcggcag cgccgacggc cgacgagacg
     1141 gaggccgccg cggcgctgct gctcgaccgc atcgaccgcg actcgcgccg cacgcgggcg
     1201 cagttcgacg agcgattcgc ggcgttcgag acggcgcgga tcggcacgct gttcgctcgt
     1261 ctgttcggcg agcgcgcggg atgagggtcc ttgccgtcta cagcatcaag ggcggcgtcg
     1321 gcaagacggc gaccgcggtc aacctggcct acctgtcggc gcgcgccggg gcgcggacgc
     1381 tgatttggga tctcgacccg caggcggcga cgacgttcta cttccgcgtc gcccccaagg
     1441 tcagcggggg aatccgcaag ctggtccgtg gcaagcgagc gatcgaccgg ttgatccgcg
     1501 gcaccgacta cgacgggctc gacctgttac cggccgactt cagctaccgc aatctcgatc
     1561 tcgagctcga cgcgacccgc aagccgcgct cgcgactcgc caagcgcatc cggccgttgc
     1621 gccggcgcta cgaccacctg tacatcgact gcgcgccgaa catctcactg gcctccgagg
     1681 gaatcttcga ggcggccgac gcgctgctgg tgccgatcat cccgaccacg ttgtcgcaac
     1741 gctcgctgcg ccagttgctc ggccacctcg accagcaggg tcccaagcgg ctgagcgtgc
     1801 tcccgttctt ctgcatggtc gaccgacgca agagcctgca ccgccagatc tgcgccgcgg
     1861 acggcggcgg atcggtgcgc tatctcgccg cccgcatccc gtactcgagc gaggtcgagc
     1921 ggatgggcgc gcggcgcgca ccgctcgcga cctatgcgcc ggcctgcgag gccgcgcagg
     1981 cgtacgaggc gctgtggcgc gaggtgctgg gccacacggg aagctggctc ggctggttcc
     2041 gcaccgatcg cttcgggttc tgatcggtca aggagtgatg cggtagccgt ggagtcgctc
     2101 gccgtcgctc gcgagcggct gcggcggagc gccgttcaag ccgacccgta tcgccccgcc
     2161 gtcggcgacg ttgagcagca ggtagcgccg gcaggtcagc tgcagctcgg tcccggcgcg
     2221 gagaccgtcg agcatctcga cgcggcggtt gtcgcagttg agcgagccgg tcaccggacg
     2281 accgatgacg atgcgggcac ggaggccgcc gttggcctcg gcgtcgaccg gcgccgtatc
     2341 gcgctcgggc tcctcgggcg gcggctcggg cacgggagcg ggacgtgccg gcacgggctc
     2401 gggaggcgtc gccgcctcgg gcaccggggc ggggtcgacg tcggctgcgg gctcgacggc
     2461 tcgcgggggt cgatcatcgc tcggcgggtc ggccgtctcc cgcagctcgc cggccgcctc
     2521 ggcctgcccg ccttcgaccg cggggcccgc gggagcggag ctcggtgccg gcgggcggcc
     2581 gctagcacct cccgactcgc cggagtcgat gagcgccgcg atgccccatg cacccaaccc
     2641 cagcgcggcg acggcgccga ccgcgatcaa cgcggtccac agcgagatcg gcagtgacag
     2701 ccgggggaac gtcgccgacg acgcggcggc gttgcggtcc gcggcgaggt gcagcgcctc
     2761 gatcgtgcgg tcctccgggg gatcgggctc ggcggagcgg cgctcggcga ggaacgcgcg
     2821 gaccgtcgca tccgggtcga gtccgaccgc ctgcgcgaac gccctgacgt agcctcgatc
     2881 gaagaccccg ccgggtagcg gttcgaagtc attctcctcg agggcgcgca gataacgcac
     2941 gccgatcttc gtcccgtcgg cgatctcctc gagcgtgatc gaacgggact cgcgggcgcg
     3001 tcgcagtttc tcgccgaaac tggccatccg gagatccggg gacgaatccg ggaaggccgg
     3061 cccactccgg cctccgagcc gccccgcacc ccccaattat aggtcgcccg cggcgaggag
     3121 gcttgtcgcg gccgggctat tccccgggtt ccgtcgccga ttcgtcaccg acgcagacga
     3181 attcggccct ggcgatcacg cggtcctccc cgtcgcgcgc ctcgaccgtc cagttccccg
     3241 ccgagccggg gtggagcgtc ttccggctgt aggtccgcca ctgggggccg cccaaggtga
     3301 gcccgatcga gatcacctct tggccttcgc ggagccacac gtgccggatc cgctcgccct
     3361 cctggccacc gacgacgcgg gtccagaaca cgaccgccgt accctcggcg aagcggtcgc
     3421 cctcgccctc gagctcccgg tcggcgattc cgctaccgac gccggactcg ggcacggaca
     3481 ggtgggattc ggctcggccc tcctcggccg ccgccggcag gaacgccgcc gacccgatgg
     3541 cgcaggcgag ccacaacgcc gcacaacgat ggttcatgcc gttcattcct ttctcgtggg
     3601 gtcgccgtcg tcgccggccg atttgtcggg gtacgctggt gggctccacc gttcgaccgg
     3661 ttgctcggcg attcgtttct cgagccagtc gattgccgcg tcgagctggg catcctcgcc
     3721 gttgaacgtg gcgtgcggca gattgtcgac gacgatgtcc ggttcgacgc cgtgaccctc
     3781 gatcagccat tcgccctcgg gcccgtagac gccgaactcg gcggcggtcg cgatgccgcg
     3841 gtcgaccagg cgattgctgc cggtcagcca gatctcgcca ccccaggtgc gcgtgccgat
     3901 caccgtcccc aggtcgagtc gtttcacgcc ctcgctgaag gcctcgccgt ccgatgcggt
     3961 gcgctcgttg cacagcacca cgacgtggcc gcggaaggcg tactgcatgt tccacgacgg
     4021 agcgtagccg gtgcgctggc tccagtggaa ccacgccttg cgcagcagcc ggctgatgat
     4081 ccagctgtcg atgttgccgc cgcgattgtg acgcacgtcg atgatcagcc cctgtcgatc
     4141 gaagaccggg tagaagccct cggcccacga cgtgtaatcg ccgccaccca tcgcgcgcag
     4201 atgcaagtag ccgatgcgcc cctcgccctt ccgctcgacc tcgagccgtc gcgtgtactc
     4261 ccactcgtgg tagcgcagat cggcggcttc gctgacggtc atcggcaccg cgatcacctc
     4321 gcgtcgcttc gatccgccgg ccggccgcac gccgagcagc agctggcggc cggcctggcc
     4381 gcgcagcagc acgccgatat cgggcacgtc gagcgtcccg cggccgttga ccgcatcgat
     4441 cacgtcgccc tccccgagct cgacgccggg ccgcgccagc gggctcgccg cctgcggctt
     4501 gtccgggtcg gagcggtaga tgtgcgcgac gcgatagccg ccagccggct cgtcccgctc
     4561 gagcaccgcc ccgagcgagg ccggcgcgat ctcgtccggc ccgtcgcgca tgtcgccgcc
     4621 cgagacgaaa atgtgcaacg cgctcagctc ggagaccatc cgggcgatga gatcggacag
     4681 ctcggcgcgc gtcgtcacgc gatcgaccaa cggacggtac ttgtcgagca tcgccttcca
     4741 gtcgacgccg tgcatcccgg ggtcgtagaa atagtcgcgt tcgagccgcc aggcctcgac
     4801 gaacatctgg cgccactcgt cgcgcgggat gatcgacagc cgccagccgg cgaggtcgac
     4861 gtcacgcttg tcgagctcgg ccttcttcgg ctcggcgtcg atgacgtaca gcttgtcgtc
     4921 cttgcgcagc agcagcttct tgccgtcgcc cgacggctcg aaaccacgca cgtcgtcgac
     4981 cagcgtctcg acctcgagcg gttggcggga gatcttgacg ccgaccagcg tgctcgtcga
     5041 ctcgccggcc ttgcgcgaca gccagaacag gtgcttgtcg ttgagcgcca ggctgccgta
     5101 attgcccggc gggagcggga cgaatccgac gcgccgctcg atgccgtcga agtcgatttt
     5161 gaccggctcg cgctcgccgc cggcttgctt gcgccccttc ttttcgtcct cgtcgtcgtc
     5221 gtcgtcgcgc tcttgctctt cgtcggccac gtcgtcgtgc agctcgtcgg gcggcgcgaa
     5281 cggcgagcgc agtccgggcc gcagggcaac gaggcagatc cgggtcgtct tgtcgagata
     5341 gggctcgggc tggtagttgc cccacgggtt gccgacgatc gagctcagct cgcgatcgga
     5401 caggaagtac agccagtcgc cgtcgggact gaacaccggt tcgtagctgt cgaaccggtc
     5461 actcgtcagc tcgcgactcg tccccgacgc gacgccccag atcttgatcc gcgagaacat
     5521 gttgtcggca gcggcgacgt aggctaacca gcgcgaatcg ggcgaccagg cgagctgccg
     5581 gaagtcggcc acccggttct cgtcgatcaa gcgattggtc cggcgctcgg tgtcgtagat
     5641 ccacaggcgc tgggacttgt cgtggtgggc caggtatcgc ccgtccggcg acggcacgac
     5701 ctcccagcgc agcacgtcgc cgtcgctcgt cagccgcgtc tcctcgccga tcccgttggc
     5761 cggcagccgc cacagctcta cctcgcccga cgcgtcggac aacgcgacga gcgtctcgcc
     5821 gtccggcagg aagcgcgcgt cgcgataacg cacgcccgcg ctgcgggtga cctcgacgaa
     5881 tcgcccctgg cgctggggcg cgacgaacac ccggccgcgc gcggtcaaca cgacccgctt
     5941 gccgtcgggc gacgggtggt aggcggtgac gtactccatc ggctcgtcga cccagcgttc
     6001 ccgcgtctgg tcgagatcgg tgtcgagccg gatcccgatc accgcgtcac gcgcggcttc
     6061 gatgcggtag gcgtggaggt cggcgccgag ctgatagacg atcgtcccgt ccgacagcga
     6121 cggcgaggcg acgtcccagc cgacgtgcgt cgtgtgctga cgcagcccac cgccgtcgac
     6181 cgtcatcgac cacaggttca tcgtgccgtc ccggtcggag acgaagtaga cgcggccgcg
     6241 ccagtacatc ggtgacttgc tcgttccggc gtagtcggtc gtcagcggct cggcttcgtc
     6301 ggcaccgtcg gcccagcgcc acagctgctc tacggtgccg ccccggtagc gcttggtgtg
     6361 gcttccctgg aacggcagtc gggtgaagta cagcgtcgtc ccggcatcgt cgaacacccc
     6421 gtcggcggcc tgcgccagcg ggatccgctc gagcgcaccg tcgtcggggt cgagcagcac
     6481 gagttgccag tcggggagcg tggcgaacga ctgggtcgaa aagagcacgc gaccgtcggg
     6541 cgtccagccc gcgacggaca ccccgctcgc gccgtacgtc cggcgggtag gcagcccgcc
     6601 gtcgaacggc atcgtgtaca gctcgctcgg cccctcgtac tcggcgacga aggcgaccgt
     6661 tcgcccgtcg ggcgagatcg acgaccgacc ctcgtccccc gggtggctgg tcagccgccg
     6721 ggcgcgaccg ccgtccagcc cgaccttcca cagatcgcct tcggcggcga agacgatcgt
     6781 ctcgccgtgg atcgcgggct cgcggtagta gccgagcggg ggctcggcgc gtgcacccgg
     6841 gaagacgcct gccagcagca acgacgcgag caaccgcgac agcgaacgca tgaccgacct
     6901 ccagacccgc cgggcgtcgg c
//
LOCUS       DASPGN010000057         5717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_713990, whole genome shotgun sequence.
ACCESSION   DASPGN010000057 DASPGN010000000
VERSION     DASPGN010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713990"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..827)
                     /locus_tag="VD788_01725"
     CDS             complement(42..827)
                     /locus_tag="VD788_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXV75010.1"
                     /translation="MNFAGAARLGLATQFAWFHGETEPAVSLICDRLVPAAADGLART
                     GIDRDDIERYLGIIERRVASHRTGAEWLIQSLGEMRGQGTIGERLTALTAATVKRQAD
                     NRPVSEWETARIDEAGGWRRTYLKVEQYMTTDLFTVGEDESLELVANVMAWKRIRHIP
                     VEDNRHRLIGLVSYRSLLSHFAKQGTRQADRHVAVSRLMKRDPITISPDQSILDAITT
                     MRENKIAALPVVKDGRLLGIITERDFMNMAAELLYEQIGRLTD"
     gene            complement(736..1965)
                     /locus_tag="VD788_01730"
     CDS             complement(736..1965)
                     /locus_tag="VD788_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75011.1"
                     /translation="MGRQTIDSEVGAKRRREFMRCLLDDLTALEFMLERDMIEADLRR
                     IGAEQELFLVDSHYRPAPAALAILGAIDDPRFTTELGLFNLEINLSPRVYGGRCLSEL
                     EQELLEATAKLNETARKQGVAPVLCGILPTLRKSDLGLDNMTPTERYRALNEAMSTLR
                     GGAYEFHIKGLDELLLKHDSVMLESCNASFQVHFQVGGREFPNLYNIAQVVAAPVLAA
                     GANSPLLFGRRLWRETRIALFQQAIDTRSSMHFLRERSPRVHLRRPLGRTVGARAVPG
                     RHLTIPHAGHGGERAGRDRDARGGSDPRTAVVVSVQQHGLPLEPRMLRRDRRQAALTH
                     RESRAPVRTDRRRRSGQRGLLVRLDRRAGEQDRRRDPADLVRGRQDELRRSGASRARH
                     AVRLVPRRNRTGGQPDL"
     gene            complement(2086..2997)
                     /locus_tag="VD788_01735"
     CDS             complement(2086..2997)
                     /locus_tag="VD788_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75012.1"
                     /translation="MSTEPFRGRLERLAVSPGALTADLVVGRWIVRFEGLADADRDEL
                     GHRWGPFLREPAGTEPALRIRVLDAGADAWLGPPRVRGERYRLELEGDRPAGLIVSHN
                     FALDRNVTGGAPWRAGISAPTREPRGRIVENVARLALAELALAAGGFALHAAGVLHAA
                     KAWLYCGPSRSGKSTAVALSVPARSLGDDFAVLAPRAGEWSAAAVPFDGAERVGRAAP
                     RGEFPVAAAWRLYRGDGTRVERPEPRIATASLMACAAFPWLYPDRAADLLDRIDRFAA
                     ETGFAHLHFSSGSDLYRELLALTPSGR"
     gene            3086..4360
                     /locus_tag="VD788_01740"
     CDS             3086..4360
                     /locus_tag="VD788_01740"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HXV75013.1"
                     /translation="MRLKDTRPLNRSVLLNYFLAAATPRSGWQVGMELEKMGRDAASG
                     AALPYDGDGPSVLSVIEAIRAARGGDPILEADKLIGLDAGWGTISLEPGGQVEWSSRP
                     YASLDDLRAALDEHLRTMVLVGRQVGVRWVGEAVDPRLPVEAMPWMPKARYEIMRPYL
                     GERGELAHRMMTQTASIQCAFDYSDPDDWARKFRAAALVTPLAVALFANSSRIDDRDS
                     GYVSFRQAIWRRTDPARCGLPAIVFDDGFGPEAWLDWVLEVPTMFLHRARGLVPVGGV
                     PFSRLLERVGCTAVKQEDWETHISTVFTEVRSYDYIEVRSADLQADPLAFSVPSFWTG
                     LLYHADSLDAALELRRVVRSHADWLSAMDSAARHGLVGTIGGHPIGALAAEMLSASLK
                     GLRGGAACAGSSGAAARAVELLAEHRSLEVAA"
     gene            4427..>5717
                     /locus_tag="VD788_01745"
     CDS             4427..>5717
                     /locus_tag="VD788_01745"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HXV75014.1"
                     /translation="MNAIATYLERNRERHLAELVELLSIPSISTDSSHAGDVRRAAEW
                     LHRRLDRAGCSLVETIATGGHPIVYGEWSGAPGRPTILVYGHYDVQPVDPLHQWTSPP
                     FEPEVRDGQLYARGAADDKGQMIVHVNALEAHLAVAASAPVNVKFLIEGEEEIGSKHL
                     EPFVRDNRDRLACDAIVVSDTTMFAKGIPSICYGLRGLTYLEIAVRGSDRDLHSGSYG
                     GAVINPANALVGMLARLKDAKGRVTIPGFYDKVRRLSVPERRSFNALPHSDRRFQQAI
                     GAPALFGESGYSTLERVWVRPSLDINGIWAGFTGEGAKTVIPAEAHAKLSMRLVPDQT
                     PKEIARKAMAYLKQLAPAAVKVTVREVHGGDAWVAVTDHRALVSASAAVKRAFGREPV
                     YVREGGSIPIVATFKKLLRVPVVLMGLGLPDDGLHAPN"
BASE COUNT          871 a         1963 c         1983 g          900 t
ORIGIN      
        1 gggcctcgac cgggccagtg gattcgtcgg acacctgcgg ctcagtctgt cagccggccg
       61 atctgctcgt acaacaactc ggccgccatg ttcatgaagt cacgctcggt gatgataccg
      121 agcaaccgcc cgtctttgac gacgggtagc gccgcgatct tgttctcccg catcgtcgtt
      181 atcgcgtcga ggatcgactg gtcgggagag atcgtgatcg gatcgcgttt catcagccgc
      241 gagacggcga cgtgtcggtc cgcctggcgc gtcccctgct tggcaaagtg cgacaacaac
      301 gaccggtagg ataccagacc gatcagccgg tgccggttgt cctccaccgg aatgtgccgt
      361 atccgcttcc aggccatcac gttcgccacc agctcgagcg attcgtcctc gccgacggtg
      421 aacaggtcgg tcgtcatgta ctgttcgacc ttgaggtagg tccggcgcca tccgccggcc
      481 tcgtcgattc gtgccgtctc ccattcgctg accggacggt tgtcggcctg gcgcttgacg
      541 gtcgccgccg tcaacgcggt caaccgctcg ccgatcgtgc cctggccgcg catctcgccg
      601 agcgattgga tcagccactc cgcgccggtg cggtggctcg ccacgcgtcg ctcgatgatc
      661 cccaggtatc gctcgatatc gtcgcgatcg atgccggtgc gcgccagtcc gtcggccgcc
      721 gcgggcacca accggtcaca gatcaggctg accgccggtt cggtttcgcc gtggaaccag
      781 gcgaactgcg tggcgagccc gagacgcgcc gctccggcga agttcatctt ggcgtcctcg
      841 aacgagatca gctgggtcac gtcgtcggtc ttgctcgcca gcgcgccgat caagccgaac
      901 caaaaggccg cgttggccac ttcgtcggcg acggtcggtc cggacgggag cacgcgattc
      961 tcgatgcgta agtgcggctt gccgtcggtc acgccgtagc atgcgcggtt ccagcggtag
     1021 accgtgctgt tgaacagaca caacgaccgc agttcgggga tcgctcccgc ctcgagcatc
     1081 gcggtcgcgt ccggctcgtt ctccgccgtg accagcgtgc ggaatcgtga gatgtcgtcc
     1141 tggaacagct cgagcgccga ccgttcgacc caacggtcgc cgaaggtgaa cccgcgggct
     1201 gcgctcgcgg aggaagtgca tcgagctccg ggtgtcgatc gcctgctgga acagcgcgat
     1261 ccgcgtctcg cgccacagcc ggcggccgaa caacagcggc gaattcgcac cggccgccag
     1321 taccggggcc gcgacgacct gagcgatgtt gtacaggttg gggaactcgc gcccgcctac
     1381 ctgaaaatgg acttggaagc tcgcgttgca cgattcgagc atcaccgagt cgtgcttgag
     1441 cagcagctcg tcgagaccct tgatgtggaa ctcgtacgca cctccccgga gcgtgctcat
     1501 cgcttcgttc aacgcgcgat agcgctcggt cggggtcatg ttgtcgagcc ccaggtcgga
     1561 cttgcgcaac gtcggcagga tgccgcacag caccggcgcg accccctgct tccgcgcggt
     1621 ctcgttcagc ttggccgtcg cctcgagcag ttcctgctcc agctccgaca ggcatcgccc
     1681 gccgtagacc cgcggcgaga ggttgatctc gaggttgaac agaccgagct cggtggtgaa
     1741 gcgcgggtcg tcgatcgcgc cgaggatcgc cagcgccgcc ggcgccggac ggtagtgcga
     1801 gtcgacgagg aacagctcct gttccgcgcc gatgcggcgc aggtcggctt cgatcatgtc
     1861 acgctcgagc atgaactcga gtgcggtcag atcatcgagc aggcaacgca tgaactcgcg
     1921 tcgccgcttc gcgccgactt cgctgtcgat cgtctgacgg cccactggcc tcccgcggcg
     1981 ttggacgccg gccgtgaggg accgccgctc tctcgccccg atcctatccg tcacacgcgg
     2041 acggcgtcaa tccacaccgt tgtttacggc gggacgcgcg cgccgtcaac gaccgctcgg
     2101 cgtcagagcg agcagctcgc ggtacagatc cgagccggac gagaaatgga gatgagcgaa
     2161 ccccgtctcc gccgcgaacc ggtcgattcg atcgaggagg tcggcggcgc ggtcggggta
     2221 gagccagggg aacgccgcgc acgccatcag cgaggccgtt gcgatccggg gctccgggcg
     2281 ctcgacccgc gtgccgtcgc cccgatacag acgccaggcg gccgcgacgg gaaactctcc
     2341 ccgcggcgcc gcacgcccga cccgctcggc gccgtcgaac gggacggcgg cggcggacca
     2401 ctcgccggca cgcggcgcga gcacggcgaa atcgtcgccg aggctccgtg ccgggacgct
     2461 caacgccacg gccgtcgact tgcccgaacg cgacggcccg cagtagagcc acgccttggc
     2521 cgcgtgcagt accccggcgg cgtgcagggc gaacccaccg gcggcgagcg ccaactcggc
     2581 cagcgccagg cgggcgacgt tttccacgat gcgcccgcgc ggttcccgcg tcggtgcgct
     2641 gatgccggcg cgccacggtg cgccacctgt cacgtttcga tcgagcgcga agttgtgcga
     2701 gacgatcagg ccggcgggcc ggtctccctc gagttcgaga cggtagcgct cgccgcgcac
     2761 tcgcggcgga ccgagccagg cgtccgcgcc ggcgtcgagc acgcggatcc gcagggctgg
     2821 ctccgtcccc gcgggctcgc gcaggaacgg tccccagcgg tgaccgagct cgtcgcggtc
     2881 cgcgtcggcc agaccctcga aacggacgat ccagcgaccg acgaccagat cggccgtcag
     2941 cgcgccggga gagaccgcca gccgctctaa ccggccgcgg aacggttccg tactcaacga
     3001 cgtttcctcg cgtgggcgga ggctcgagat ccgcgccggc ggcgagccgc gcgacacggg
     3061 ttagaatagc gcgtgagagg gagcaatgcg cctcaaagac acgcgaccgc tgaaccgctc
     3121 ggtcctgctc aactacttcc tcgcagcggc gacgccgcgg agcggctggc aagtcggcat
     3181 ggagctggag aaaatggggc gcgacgcggc gagcggcgcg gcgctgccct atgacggtga
     3241 cgggccgtcc gtgctgtcgg tgatcgaggc gatccgggcg gcgcgcggcg gcgatccgat
     3301 tctcgaggcg gacaagctga tcggtctcga cgccggctgg ggcacaatct cgctcgagcc
     3361 gggcgggcag gtcgagtggt cctcgcggcc gtacgcctcg ctcgacgacc tgcgggccgc
     3421 gctcgacgag caccttcgca cgatggtcct cgtcggacga caggtcggcg tgcgctgggt
     3481 cggcgaagcc gtcgatccgc ggctgccggt cgaggcgatg ccgtggatgc ccaaggcgcg
     3541 ctacgagatc atgcgaccgt atctcggcga gcgcggcgag ctggcgcacc ggatgatgac
     3601 ccagaccgcg agcatccagt gcgcgttcga ttactccgac cccgacgact gggcgcgcaa
     3661 gttccgcgcc gccgcgctgg tgacgccgct ggccgtcgcg ttgttcgcga actcgtcgcg
     3721 gatcgacgac cgcgacagcg gctacgtctc gttccgacag gcgatctggc gtcggaccga
     3781 tcccgcgcgg tgcgggctgc ccgcgatcgt cttcgacgac gggttcggcc cggaggcttg
     3841 gctggactgg gtgctcgagg tcccgacgat gttcctgcac cgggctcgcg ggctggtacc
     3901 cgtcggcggc gtgccgttct cccggctcct cgagcgcgtc ggctgcaccg cggtcaagca
     3961 ggaagactgg gagacccaca tctcgacggt gttcaccgag gtacgctcct acgactacat
     4021 cgaggtgcgc agcgccgacc ttcaagccga tccgctggcg ttttcggtgc cttcgttctg
     4081 gaccgggctg ttgtaccacg cggactcgct cgacgccgcg ctcgagctgc ggcgagtcgt
     4141 gcgcagccac gccgactggc tgtcggcgat ggacagtgcc gcgcggcacg gcctggtcgg
     4201 gacgatcggc ggccacccga tcggcgcgct ggccgccgag atgctgtcgg cctcgctcaa
     4261 ggggttgcgc ggcggggcgg cttgcgccgg aagctcgggg gccgccgccc gcgccgtcga
     4321 gttgctggcc gagcatcgct ccctcgaggt cgccgcctga cgcgccgcga tcgcggccgc
     4381 cgcccgagtc cgcaacgacc tgttcgatcc agctggagac cgatcgatga acgccatcgc
     4441 cacctacctc gagcggaatc gcgaacgcca cctagcagag ctggtcgagc tgctgtcgat
     4501 cccctcgatc agcaccgact cgtcgcatgc cggcgacgtg cgccgcgcgg cggagtggct
     4561 ccaccggcgg ctcgatcgtg ccggctgctc gctcgtcgag acgatcgcca ccggaggcca
     4621 cccgatcgtc tacggcgagt ggtcgggggc ccccggtcgc ccgacgatcc tcgtctacgg
     4681 tcactacgac gtccagccgg tcgatccgct gcatcagtgg acgagcccgc cgttcgagcc
     4741 ggaggtgcgg gacggccagc tctacgcgcg cggcgcagcg gacgacaagg gccagatgat
     4801 cgtacacgtc aacgcgctcg aggcgcacct ggcggtcgcc gcgagcgcgc cggtcaacgt
     4861 gaagttcctg atcgagggtg aagaggagat cggttcgaag cacctcgaac cgttcgtccg
     4921 cgacaaccgg gaccggctgg cctgcgacgc gatcgtcgtt tccgacacga cgatgttcgc
     4981 caaggggatt ccgtcgattt gctacgggtt gcgcggtttg acctacctcg agatcgccgt
     5041 gcgcggcagc gatcgggacc tgcattcggg cagctacggc ggggccgtga tcaatccggc
     5101 caacgcactg gtcggtatgc tggccaggct gaaagacgcc aagggtcggg tgacgatccc
     5161 ggggttctac gacaaggtac ggcgactgtc ggtgcccgag cgccggtcgt tcaacgccct
     5221 gccccactcg gatcgcaggt tccagcaggc gatcggtgcg ccggcgctgt tcggcgagtc
     5281 gggctattcg acgctcgaac gcgtctgggt gcggccgtcg ctcgacatca acgggatctg
     5341 ggccgggttc accggtgagg gcgcgaagac ggtgattccg gccgaggcgc acgccaagct
     5401 gtcgatgcgg ctggtgcccg atcagacgcc gaaggaaatc gcgcgcaagg cgatggccta
     5461 cctcaagcag ctggcgccgg cggcggtcaa ggtgaccgta cgcgaggtgc acggtggcga
     5521 tgcttgggtt gcggtgacgg accaccgcgc gctggtcagt gcatccgccg cggtcaagcg
     5581 tgccttcgga cgcgaaccgg tctacgtgcg cgagggcgga tcgattccga tcgtcgccac
     5641 cttcaagaag ctgctgcggg tcccggtcgt gctgatgggc ctcggcttgc ccgacgacgg
     5701 gcttcacgcg ccgaacg
//
LOCUS       DASPGN010000058         2650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_128178, whole genome shotgun sequence.
ACCESSION   DASPGN010000058 DASPGN010000000
VERSION     DASPGN010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128178"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VD788_01750"
     CDS             complement(<1..424)
                     /locus_tag="VD788_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HXV75015.1"
                     /translation="MATTDKAPVVDVAQRVEFGKNANRRLRAAGNIPGNVYGLGLEPY
                     SVSVSPRRIDEILKSASGRNSIIRLSLAGGKASRAVMLREIQRDPVSEQLLHIDFVRV
                     DENKPVHVRVPVHLVGTPIGVKNEGGVLDFVHRDVEVSC"
     gene            complement(448..1389)
                     /locus_tag="VD788_01755"
     CDS             complement(448..1389)
                     /locus_tag="VD788_01755"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXV75016.1"
                     /translation="MKSELKVFSGNGNPTLARAICEYLRLPLGKWQLTRFADGEVYCQ
                     ILENVRGTDVFVIQPTCRPVNEHLVELLIAVDALKRSSAARITAVLPYYGYARQDRKD
                     KPRVPISAKLVADLLQAAGVDRVLAMDLHAPAIQGFFDVPVDHLLAAPVLLDWIDRQR
                     YDNLMIVSPDAGGVERARFYAKRLGADLAIIDKRRVVANVAETMNVIGDVDGRTCVIV
                     DDLVDTAGTLVGSAKALLDHGAVSVVACFTHALLSGPALERVALPEIERVIVTDTIPL
                     RDEAAEVENITVLSVASLLGEAIARIHSNSSVSSLFV"
     gene            complement(1449..1523)
                     /locus_tag="VD788_01760"
     tRNA            complement(1449..1523)
                     /locus_tag="VD788_01760"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1489..1491),aa:Gln,seq:ttg)
     gene            complement(1721..1963)
                     /gene="spoVG"
                     /locus_tag="VD788_01765"
                     /pseudo
     CDS             complement(1721..1963)
                     /gene="spoVG"
                     /locus_tag="VD788_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
     gene            complement(1983..>2650)
                     /locus_tag="VD788_01770"
     CDS             complement(1983..>2650)
                     /locus_tag="VD788_01770"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75017.1"
                     /translation="LGGTVGAALHLRKRIPVQGGLGGGSSDAAGTIRLLERLWGHELR
                     TETRWRVAAALGADIPFFLTGGTARGRGRGDRIEPLPPLAEQSLVLGLPPFGVPTADA
                     FREVHARLTLPGIGVSLPRLSAHKWPGEKDFSFAANDLELVVFPRYPELRRFRDALLS
                     AGARVALLSGSGSTLFGVFDDGPAALDGAARLGRGFPAWNVVPTRTVDRPAGEPDDAG
                     GDG"
BASE COUNT          447 a          923 c          854 g          426 t
ORIGIN      
        1 gacaggagac ctcgacgtcg cggtggacga agtcgagcac gccaccctcg ttcttgactc
       61 cgatcggcgt tccgaccagg tgcaccggca cccgtacatg caccggtttg ttctcgtcga
      121 cgcggacgaa atcgatgtgc agcagttgct cgctcaccgg atcacgttgg atctcgcgca
      181 gcatcaccgc gcgcgaggcc ttgcccccgg caagcgacag ccggatgatc gagttccgac
      241 cgctggcgct cttcaggatc tcgtcgatcc ggcgcggact gacgctgacc gagtacggct
      301 cgagacccag cccgtagacg ttgcccggga tgttgccggc ggcgcgcagg cgccggttgg
      361 cgttcttgcc gaactcgacg cgctgcgcaa cgtcgacgac gggtgcttta tcggttgttg
      421 ccataccttg cgttccttcc tcgaggttca aacgaacagg gagctgaccg agctattgct
      481 gtggatccgc gcgatggcct cgcccaacag gctggccacc gacagcacgg tgatgttctc
      541 gacctcggcc gcctcgtcgc gcagcgggat cgtgtcggtc acgatcaccc gctcgatctc
      601 cggcagcgcc acgcgctcga gcgcgggacc cgacagcaac gcgtgggtga agcaggccac
      661 gaccgatacc gcgccatggt ccaacagcgc cttggccgag ccgacgagcg tgcccgccgt
      721 atcgaccagg tcgtcgacga tgacgcaggt ccggccgtcg acgtcgccga tgacgttcat
      781 cgtttcggcg acgttggcca ccacgcgacg cttgtcgatg atcgccaggt cggcgcccag
      841 acgcttggcg tagaaccgcg cgcgctcgac gccaccggcg tccggggaga cgatcatcag
      901 gttgtcgtac cgctgtcgat cgatccagtc gagcaggacg ggcgccgcta gcaagtgatc
      961 gaccggtacg tcgaagaaac cctgaatcgc gggtgcgtgt aggtccatcg ccagcacccg
     1021 atcgacgccg gccgcctgca gcagatcggc gaccagtttc gccgagatcg gcacccgcgg
     1081 cttgtccttg cggtcctgcc gggcataacc gtagtacgga agcaccgccg taatccgcgc
     1141 ggcgctcgag cgcttcagcg catcgaccgc gatcaacagc tcgaccaagt gctcgttgac
     1201 cggccggcag gtgggttgga tgacgaagac gtcggtgccg cgaacgttct cgaggatctg
     1261 acagtagacc tcgccgtcgg cgaaacgggt gagctgccac ttgccgagcg ggaggcggag
     1321 atactcgcaa atcgcccgcg ccagggtggg gttgccgttg ccgctgaaga ccttgagctc
     1381 gctcttcatc cgtcgtctcg ccgcgagggc ccgaactcac tcggctcgcc gcccgcttgg
     1441 acgcgaggtg gctggggcgg gaggattcga acctccggat gccgcttcca aagagcggtg
     1501 ccttaccact tggcgacgcc ccaacgcttc ggacgccccc gacggccacc cgatctccac
     1561 gggatcacga ttccccgggg ttgccgaagg gcggttcgac gaacccgggg cgttcgactc
     1621 tatcacgcgg gcccgagggc tcgccgccct cgtctggccc acgcgcggcg tcgagatcgc
     1681 gcaccggcac gtcggccgag cctcccgagt cggcgacgac gcgccgatac tcaccgatga
     1741 tggtctcctc gatgcggcgc cgggtctcgt tgttcaacgg gtgcgcgatg tcgcgaaagg
     1801 tcccgttcct gcgtttcttg ctcggcatcg aaacgaacaa gccgtggttg ccctggatca
     1861 ccttgatgtc gctgaccgcg aagcagtcgt cgaacacgat ggagacgaag gctttcagct
     1921 tgtcttcgtc gacgagaaaa acccggatct gcgtgatctc catctgaccg acccccgccc
     1981 gttcatccgt ccccacccgc gtcgtccggt tctcccgcgg gtcggtcgac cgttcgcgtc
     2041 gggacgacgt tccaagcggg aaacccccgg ccgagtcgcg ccgcaccgtc gagcgcggcc
     2101 ggaccgtcgt cgaacacccc gaacagggtc gaaccgctgc cactcagcaa ggccacccgc
     2161 gcgcccgcac tcaacagcgc gtcgcgaaaa cgtcgcaact ccggataccg tggaaagacc
     2221 acgagctcca gatcgttcgc cgcgaacgag aagtcttttt ctcccggcca cttatgcgca
     2281 gagaggcgag gcaaactaac accgattccg gggagtgtca accgggcgtg cacctcgcga
     2341 aaggcgtcgg cggtcgggac tccgaacggc ggaagaccga ggaccagcga ctgctcggcc
     2401 agcggcggga ggggctcgat ccggtcgccc cgtccgcgtc ctcgggccgt cccgcccgtc
     2461 aggaagaacg gaatgtcggc cccgagcgcg gcggcgactc gccagcgggt ctccgtgcga
     2521 agctcgtgtc cccacaatcg ctcgaggagt cggatcgtac cggcggcgtc cgagctgcct
     2581 ccgcccagcc cgccctggac cgggatccgc ttgcgcagat ggagcgccgc gccgaccgtg
     2641 ccgcccaaac
//
LOCUS       DASPGN010000059         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1651393, whole genome shotgun sequence.
ACCESSION   DASPGN010000059 DASPGN010000000
VERSION     DASPGN010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1651393"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VD788_01775"
     CDS             complement(<1..351)
                     /locus_tag="VD788_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV75018.1"
                     /translation="MANGKRKASAEAGTPADGAMPGAERVVGVLVVDDEFSVRDSLEG
                     WFRKDGYRTSSAKDAHHALRKLQDEPWDVVLLDIKMPGMDGMELQRRIHEIDPEVVVI
                     MITAYASVESAVQAL"
     gene            complement(320..2002)
                     /locus_tag="VD788_01780"
     CDS             complement(320..2002)
                     /locus_tag="VD788_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV75019.1"
                     /translation="MTALRPDRAGLAWVKSLYGSLSLSLFFWLFGTIIVAFACFAWVT
                     LRYSEQQWNRNVLAGASRFSDLIQSSTHYSMLLNRKADVRHIIETVAREPGVEGVRIY
                     DKLGTVVFSADRDEIGRAVDLQAEACVICHQAGRPLGKVPADTQARVYHDPTRGRVMG
                     LIDPIENSRECAAAGCHAPVGEQTVLGVLDVKMSMAEADATLAALRRQIAVAGALLGL
                     LAGVAAALLTYRNVHRPVHELIRGTRRVAGGDLDTVIRVERSNEIGQLAEAFNSMTGD
                     LRRARAELTEWSDRLESRLLSRTEELNRTQRQVVHMEKMASLGKLAATVAHELNNPLA
                     GILNYARLVDRSVGELDIREADRQELSRCLRLIQKEADRCGTTVRNLLLFARHSGAEP
                     ALVPLAPVIGRSLQLIRHHLEMSGIELVSRLFESGDDSIICDANQIEQAVVALLVNAV
                     EAMPDGGRLTVAADGRASSVEIRITDEGCGIPREIQPQIFEPFFSTKSRAGGVGLGLS
                     VAYGIVKRHGGEIDVQSEVGRGTTFVIELPRRPSPHDTQESHDGKRETESVG"
     gene            complement(2064..>2292)
                     /locus_tag="VD788_01785"
     CDS             complement(2064..>2292)
                     /locus_tag="VD788_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75020.1"
                     /translation="LVKVRLSNPKAALSNLLSGKLAVDWMEQSATALRERMTGRVGPV
                     LQDGGVPVSGIARELSEGEWERVAREFLLTD"
BASE COUNT          364 a          811 c          746 g          371 t
ORIGIN      
        1 caacgcctgg accgcgctct ccaccgacgc gtaggccgtg atcatgatga cgacgacctc
       61 ggggtcgatc tcgtggatgc gccgctgcag ctccatcccg tccatcccgg gcatcttgat
      121 gtcgagcagc accacgtccc acggctcgtc ctgcagcttg cgcagcgcgt ggtgggcatc
      181 cttggccgac gaggtgcggt agccgtcctt gcggaaccag ccctcgagcg agtcgcgaac
      241 cgagaactcg tcgtcgacga cgagtacgcc gacgactcgc tcggccccgg gcatggcgcc
      301 gtcggccggc gttccggctt cagccgacgc tttccgtttc ccgtttgcca tcgtggctct
      361 cctgcgtgtc gtgcggagac ggtcggcgcg gcagctcgat gacgaacgtc gtgccccggc
      421 cgacttccga ctgcacgtcg atctcgcccc cgtgtcgctt gacgatgccg taggccaccg
      481 acaggccgag accgacgccg ccggcacggc tcttcgtcga aaagaacggc tcgaagatct
      541 gcggctggat ctcgcgcggg ataccgcacc cctcgtcggt gatgcggatc tcgaccgacg
      601 aggcacggcc gtcggccgcg accgtcagcc gcccgccatc gggcatcgcc tcgacggcgt
      661 tgaccaagag cgcgaccacc gcctgctcga tctggttcgc gtcgcagatg atgctgtcgt
      721 ccccggattc gaacaagcgg ctgaccagct cgataccgct catctcgagg tgatggcgga
      781 tcagctgcaa cgaccggccg atgaccggcg ccaacggcac cagcgccggc tcggccccgg
      841 agtgacgtgc gaacagcagt agattgcgca ccgtggtgcc gcagcggtcg gcctcctttt
      901 ggatcaacct caggcagcgc gacagctcct gacggtcggc ctcgcggatg tcgagctcgc
      961 cgacgctgcg gtcgacgagc cgcgcgtagt tgaggatccc ggccagcggg ttgttcaact
     1021 catgcgcaac cgtcgccgcg agcttgccca gcgacgccat cttctccatg tgaacgacct
     1081 ggcgttgggt gcggttcagc tcctcggtcc ggctgagcag tcgcgactcc agccggtccg
     1141 accactcggt cagctccgcg cgcgcacgcc gtagatcgcc ggtcatgctg ttgaacgcct
     1201 cggcgagctg cccgatctcg ttgctgcgct cgacgcggat cacggtatcg aggtcgccgc
     1261 cggcgacgcg acgcgtaccg cggatcaact cgtggaccgg gcggtgcacg ttacggtagg
     1321 tgagcaaggc cgccgcgaca ccggcgagca gtccgagcaa cgcgccggcg acggcgattt
     1381 ggcgccgcag cgcggccagc gtcgcgtcgg cctcggccat cgacatcttg acgtcgagca
     1441 cgccgagaac ggtttgttcg ccgaccggcg catggcaacc ggcggcggcg cactcgcggg
     1501 agttttcgat cggatcgatc agccccatca cccgaccgcg cgtcgggtcg tgatagaccc
     1561 gcgcttgcgt atcggccgga accttgccga gcgggcgccc ggcctgatgg cagatcacgc
     1621 aggcctcagc ctgcaggtcg accgcccggc cgatctcgtc ccggtcggcc gagaagacga
     1681 ccgtgcccag cttgtcgtag atccgcacgc cctcgacacc cggttcgcgg gccacggtct
     1741 cgatgatgtg ccgcacgtcc gccttgcggt tgagcagcat gctgtagtgg gtcgagcttt
     1801 ggatcaggtc gctgaaacgg ctggcgccgg ccagcacgtt gcgattccac tgctgctcgg
     1861 agtaacgcag cgtgacccag gcgaagcagg caaaggcgac aatgatggtc ccgaacagcc
     1921 agaagaaaag actcaggctc agcgagccgt agagcgattt gacccaggcc aatcccgccc
     1981 gatcgggacg tagtgcggtc attgcacctg cctacaccgt cggcgaacca cgggcggtta
     2041 agcgggcaag gaccgtgcca gggtcagtcg gtcagcagga actcccgtgc gacccgctcc
     2101 cactcgccct cggacagctc gcgtgcgatt ccgctgaccg ggaccccgcc gtcctgcagc
     2161 accggtccga cgcggccggt catccgttcg cgcaacgccg tcgcgctctg ctccatccag
     2221 tcgacggcca gcttgccgga cagcaggttc gacagcgccg ccttggggtt gctcagccgc
     2281 accttgacca gc
//
LOCUS       DASPGN010000060         5345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1065876, whole genome shotgun sequence.
ACCESSION   DASPGN010000060 DASPGN010000000
VERSION     DASPGN010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065876"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1140)
                     /gene="yvcK"
                     /locus_tag="VD788_01790"
     CDS             complement(124..1140)
                     /gene="yvcK"
                     /locus_tag="VD788_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HXV75021.1"
                     /translation="MRREKTDPNRLPDRVVALGGGTGLPAVLRGLRQLVVDGEMADLT
                     AIVAMSDDGGSSGRLRRSRGLPPPGDVRNCLVALSAEEDLLAGLFQHRYSGAEELGGH
                     NLGNLILAALAEQTGSFLKAVEVSSRVLRTVGRILPVTLEDVSLVATLEDGSRLVGET
                     AIGLCRKQIRSVTLHPRTARPAPESVEAIVQADLVVIGPGSLFTSVLPTLVLDDVARA
                     LRDTRAVRVLVGNLVSERGEAAGLTLDDHVRIIEAHAGGAVVDAILVHHGSIDDATLA
                     RYQAEGASPLVLQDGGPAGPRVIRRNLLAEGVKLRHDPAATAAGLLLAWHELSRPNPV
                     RQRS"
     gene            1260..2351
                     /locus_tag="VD788_01795"
     CDS             1260..2351
                     /locus_tag="VD788_01795"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXV75022.1"
                     /translation="MRTVIHPADEIVRRVLRDPGQMDSLSAPQWDQLLRRARRAGVLY
                     RIAVLADGALHGDGVPDRVRHQLQSARMVAARHDRSVRWEVARIRRALRPTGERFVLL
                     KGAAYVIAGLPAASGRISTDVDVLVAEDKLAVVEAALLGAGWTAMKLHPYDQRYYRRW
                     MHELPPLRHATRGTVVDVHHTILPRTSRFRPKGSKLLENAVRIDDDVSVLAAEDMVLH
                     AATHLFADGDLAGGLRELTDIDAMLRHFPTTVDRFWHRLVPRSLELDLQRPLFYALRF
                     CRRSLDTPVPDEVVAASADGRPSWPALTVMDRLVDRALLPEADGNSFRTEASLWMLYV
                     RSHWLRMPPWLLSRHLTRKAIRRLFGRSG"
     gene            complement(2702..3667)
                     /locus_tag="VD788_01800"
     CDS             complement(2702..3667)
                     /locus_tag="VD788_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75023.1"
                     /translation="MPVEALEKRRLVQADDHHVAFPHDLVAGRSHQRGDMRNTLFDES
                     TIRTEQSRDVDFRIVDLQVESLADEPLGHRDQRAFPQIVGAALEAETDHADRSAPASQ
                     HHLDRVLDVKVVALLDRRQHRRVDVAPPGRVEQRPQVLRQTRATEREPRLEIGRRDVE
                     LPVVADQRHDRMRVDADGLADSPDLVGEGDLQRVEGVAGVLQHLGGRDRGADELARQV
                     PEQLAQQILRTLSVDADDGERRAIVILDRRAFAEELGLKAEAEAHAGGHSGVLLDQRP
                     DQLVDRARQHGRTHDEQVAFALRADRLAELLRESADRGLVLATVR"
     gene            complement(3786..5009)
                     /locus_tag="VD788_01805"
     CDS             complement(3786..5009)
                     /locus_tag="VD788_01805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV75024.1"
                     /translation="MPRRLIFVSPRFLFPADSGGKIRTTQILRGMKHGPFELTLVSPE
                     PPAAGDRFRRELDEVCDRFVGWNETRRGPLFRLTRLRHALAREPIPVATDRSRDGRAA
                     IERQLASEPDVAVFDFAHAAVLAPDAIDVPTVLFTHNVESWIFRRHAEVASNPVVRWA
                     WRRQWRKMERFEQAALARFDTVVAVSDGDARFFRERYGANNVRLIPTGVDLGYFGYEP
                     PGDGDGVVFIGAMDWLANQDGVGWLLDEVWPRIAASRPTADMRVVGRQPPASLVRRAD
                     GQRWSFTGFVDDVRTHVRGRSVCVIPLRVGGGTRIKAFEAMAMGCPVVSTSIGVEGLP
                     LEDGVHYLRADTADEFSGAVLRLLVDSQLRTRISAAARRHVEEHFSARRAASVFQDIC
                     LETSNDHRSGYQQTP"
     gene            5052..>5345
                     /locus_tag="VD788_01810"
     CDS             5052..>5345
                     /locus_tag="VD788_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75025.1"
                     /translation="MSGFCGWVGLEASPEPGRHDLERMARWLNRFDGTVVDEFVDRSV
                     AVASASLTGRPALARHADLVAVLEGEPRFADADLDAEARRRGAAAAMLLGYRKH"
BASE COUNT          833 a         1820 c         1851 g          841 t
ORIGIN      
        1 tccagggatt caagccgttg catcacgcgg agcttctcgc acggcgggtc ctgcgctcgg
       61 tcggcctgcg ctacccggag ctctgcattc tcgcgtccta catgaggcgc tgaggccggc
      121 gggtcacgag cgttggcgga cggggttcgg ccgcgaaagc tcgtgccagg ccaacagcag
      181 gcccgccgcg gtcgcggcgg ggtcgtgtcg cagtttgacg ccttcggcga ggagattgcg
      241 gcgaatcacg cggggcccgg ccggaccgcc gtcctgcagg acgagcgggg aggcgccctc
      301 ggcctggtaa cgcgccaacg tggcgtcgtc gatcgaaccg tggtggacca ggatggcgtc
      361 gacgacggca ccgccggcgt gagcctcgat aatgcgcacg tgatcgtcga gggtcaatcc
      421 cgccgcctcg ccgcgctcgc tgacgagatt gccgacgaga acccggaccg ctctcgtgtc
      481 gcgaagcgct ctcgcgacgt cgtccagcac cagcgtgggc agcacgctcg tgaacaagct
      541 gccggggccg atgacgacca ggtcggcctg gacgatcgct tcgaccgact ccggcgcggg
      601 acgagccgtg cgtggatgca gcgtgaccga acgaatctgt ttgcggcaca gcccgatcgc
      661 ggtttcgccg acgagtcgcg aaccgtcctc gagcgttgcg accaagctca cgtcctcgag
      721 cgtcaccggc aggatccgac cgacggtccg cagcacgcgg ctcgacacct cgaccgcctt
      781 gaggaagctg ccggtctgct ccgcgagcgc ggcgaggatc aggttgccga ggttgtgacc
      841 gccgagctcc tccgctccgc tgtagcggtg ctggaacagc ccggcgagca ggtcttcctc
      901 ggcggacagc gcgaccagac agtttctgac gtcgcccggc ggtggaagcc ccctggatcg
      961 acgcaggcgc cccgagctgc cgccgtcgtc gctcatcgcg acgatcgcgg tcagatccgc
     1021 catctccccg tcgacgacga gctgcctcag accgcgcagg accgcgggca atccggtgcc
     1081 gccgccgagc gcgacgaccc gatcaggaag tcgattcgga tcggtcttct cgcggcgcat
     1141 gcgggaggtc catcatcgat gattccgcaa gcaatgtacg agtcgctccc ggtcgagtca
     1201 ataccgcggc ccggcgagac ttgcgcgagg gcggtcgcgg actctacagt ccgggctcgg
     1261 tgagaacggt gatccatccg gccgatgaga tcgttcgccg ggtcctgcgc gatccgggac
     1321 aaatggactc gttgagcgcg ccccagtggg accagttgct gcggcgcgca cggcgcgcag
     1381 gagtgctgta tcgaatcgcc gtgctggccg acggcgcgct tcacggggac ggcgtgccgg
     1441 acagggttcg gcaccagctg cagtccgccc gcatggtcgc ggcccgccac gaccgttcgg
     1501 ttcgctggga agtcgcccgc atccgtcgcg cgctgcggcc gaccggagag cgcttcgtgc
     1561 tgctcaaggg cgcggcgtac gtgatcgccg gtctccccgc cgcatccggc cgaatctcga
     1621 ccgacgtcga cgtgttggtc gccgaggaca agctcgccgt cgtcgaggcg gcgttgctcg
     1681 gggccggctg gacggcgatg aagctccacc cctacgacca gcgctactac cgtcgctgga
     1741 tgcacgagct gccgccgctg cgtcacgcca ctcgcggcac cgtcgtcgac gtgcaccaca
     1801 cgatcctccc gcggacgtct cgttttcgtc ccaaaggcag caagctgctc gagaacgccg
     1861 ttcgcatcga cgacgacgtc tccgtcctgg ccgcggagga catggtgctc cacgccgcga
     1921 cgcatctgtt cgccgacggc gacctggccg gcgggttgcg cgagctgacc gacatcgacg
     1981 cgatgctccg gcactttccc accacggtgg accggttctg gcatcgtctg gtcccgcgat
     2041 ccctcgagct cgatctgcaa cggccgctgt tctacgccct gcgcttctgc cggcgttcgc
     2101 tcgacacccc cgtgccggac gaggtcgtcg cggcgtcggc agacggccgg ccgtcatggc
     2161 cggcgctcac cgtgatggac cggttggtcg atcgggcact cttacccgag gccgacggca
     2221 acagcttccg aaccgaagcg tcgttgtgga tgctgtacgt gaggtcccac tggctgcgca
     2281 tgccgccctg gctgctgagc cggcacttga cccggaaggc gattcgccgt ttgttcggtc
     2341 ggtcgggcta ggaacccttg ccgaccataa tgcgctattc tgtcgcttcg accgccggct
     2401 tccggtcgag gcggtcggtg gtaccaagga ctcgcgaatg agaatcagca tcttcggtct
     2461 cggatacgtc ggagccgttt ccgcgggttg tctggcccgc gacggtcaca ccgtggtcgg
     2521 ttgcgacatc gatccggaca agctgcagct gttccgcgac gggcgcacac cgatcatcga
     2581 agcgggaatg gaacagctga tcgcgaccgc cgtggggtcc gggcgactca ccgtcaccga
     2641 ctccgccgtc gatgccgtcc gggagacgga ggtctcgttc atctgcgtcg ggacgccgtc
     2701 cctaccgaac ggtggccagg accagaccgc gatccgccga ctcgcgcagc agctcggcga
     2761 ggcgatccgc tcgaagagcg aacgccacct gttcgtcgtg cgttcgaccg tgctgccggg
     2821 cacggtcgac gagctgatcc ggccgctgat cgagcaacac tccggaatga ccgccggcgt
     2881 gggcttcggc ctctgctttc agcccgagtt cctccgcgaa ggctcgtcga tcaaggatta
     2941 cgatcgcccg ccgttcaccg tcgtcggcgt cgacggagag cgttcggagg atctgctgcg
     3001 cgagctgttc gggcacttgc cttgcgagtt catcagcacc tcgatcgcga ccgccgagat
     3061 gctgaagtac gcctgcaacg ccttccacgc gctgaagatc accttcgcca acgagatcgg
     3121 gagaatctgc caggccgtcg gcgtcgactc gcatgcggtc atgtcgctgg tctgcgacga
     3181 ccggcagctc aacatctcgc cggcctatct caagccgggg ttcgcgttcg gtggctcgtg
     3241 tctgccgaag gacctgcggg cgttgctcta cacggccagg cggcgcgacg tcgacgcgcc
     3301 gatgctggcg gcggtccagc agagcaacaa ccttcacgtc gagcacgcga tcgagatggt
     3361 gctgcgaagc cggcgccgat cggtcggcat gatcggtctc agcttcaaga gcggcaccga
     3421 cgatctgcgg gaaagcccgt tggtcacgat ggccgagcgg ttcatcggca agggactcga
     3481 cctgaagatc cacgatccgg aagtcaacgt cgcgcgactg ctcggtgcga atcgtcgatt
     3541 catcgaagag agtattccgc atatcgcctc gttgatgcga ccgacctgcg acgaggtcgt
     3601 gcgggaaggc gacgtggtgg tcgtcggcct gaacgagccg acgtttttcg agagcctcta
     3661 ccggcattcg cgcgacgacc agctcgtact cgatctggtg cggatcccgc agaccgagcg
     3721 gatccgcacc agatcgagta cgagctggtg cggatcccgc agaccgagcg gatccggggc
     3781 gagtatcacg gggtttgctg gtagccgctc ctgtggtcgt tcgaggtctc gaggcagatg
     3841 tcctggaaca cgcttgctgc gcgacgagcg gagaagtgct cctcgacgtg ccggcgcgcg
     3901 gcggcggaga tccgcgtacg gagctgcgag tccacgagca gccgcagcac ggcgcccgag
     3961 aactcgtcgg cggtgtcggc gcgcaggtaa tggacgccgt cctcgagcgg caggccctcg
     4021 acgccgatcg acgtcgagac caccgggcaa cccatcgcca tcgcctcgaa cgccttgatc
     4081 cgcgtaccgc cgccgacgcg cagcggaatc acgcagaccg agcgtcctcg cacatgggtc
     4141 cgaacgtcgt cgacgaagcc ggtgaagctc cagcgctggc cgtcggcgcg gcggacgagg
     4201 gacgccgggg gctgccggcc gacgacacgc atgtcggcgg tcgggcgcga ggccgcgatc
     4261 cgcggccaga cctcgtccaa cagccagccg acgccgtcct ggttcgccag ccagtccatc
     4321 gcgccgatga acacgacgcc gtccccgtcg cccggaggct cgtacccgaa gtaaccgagg
     4381 tcgacaccgg tggggatcag ccgcacgttg ttcgcgccgt aacgctcgcg gaagaagcgt
     4441 gcgtcgccgt cggagaccgc gacgaccgtg tcgaagcgcg ccagcgcggc ttgctcgaaa
     4501 cgctccatct tgcgccactg gcggcgccag gcccagcgaa cgacgggatt ggaggcgact
     4561 tcggcgtgac ggcggaagat ccagctttcg acgttgtggg tgaacaacac ggtcgggacg
     4621 tcgatcgcgt cgggcgccag caccgccgca tgcgcgaagt cgaagaccgc gacgtccggc
     4681 tcggacgcga gctggcgctc gatcgccgcg cggccgtcgc ggctcctgtc cgtcgcgacc
     4741 gggatcggtt cgcgcgccag cgcgtggcgc agccgcgtca gccggaagag cggcccgcgc
     4801 cgcgtctcgt tccagccgac gaaccgatcg cagacctcgt ccagctcgcg gcggaaacga
     4861 tcgccggcgg cgggcggttc cggcgagacc agcgtcagct cgaacggtcc gtgcttcatg
     4921 ccgcggagga tctgcgtcgt gcggatcttg ccgccggaat cggcggggaa gaggaatcgg
     4981 ggcgagacga agatcagtcg gcgcggcacg gtgcagtcgc ctcggtcgga accagtgcgg
     5041 gggtttgacg catgagcgga ttctgcggct gggtcgggct cgaggcaagc cccgaaccag
     5101 gccggcacga cctggagcgc atggcgcgtt ggctgaaccg cttcgacggc acggtcgtcg
     5161 acgagttcgt cgaccggtcg gtcgcggtgg ccagcgcctc gctcaccggc cggccggccc
     5221 tcgcccggca cgccgacctc gtcgcggtgc tggagggcga gccgcggttc gccgacgccg
     5281 atctcgacgc cgaggcccgc cggcgtgggg cggcggcggc gatgctcctc ggctatcgga
     5341 agcac
//
LOCUS       DASPGN010000061         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_11792, whole genome shotgun sequence.
ACCESSION   DASPGN010000061 DASPGN010000000
VERSION     DASPGN010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11792"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..539)
                     /locus_tag="VD788_01815"
     CDS             complement(78..539)
                     /locus_tag="VD788_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXV75026.1"
                     /translation="MSKQRKRAVVVVALGASVLCVVGAEGPSSDDVIAERQHTMESIQ
                     AALKEVVSIVRKEASFDAEVVRGSGTKIAELLDQASKLFPEGSEKGGIETWAKPEIWS
                     RPEGFVEALERTRDAAVSLAEVTEESKLGPALGQLGNGCKGCHDTYRRPKE"
     gene            complement(619..1143)
                     /locus_tag="VD788_01820"
     CDS             complement(619..1143)
                     /locus_tag="VD788_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXV75027.1"
                     /translation="MSDEPEVERYLDPVRRELARRLPPAELESRVVFAVRNARRRRRG
                     WRWAAAVLAGSALFGAGWISGASGLDPRAKTAPDGKYALLLFSIDDRPGADANGRVRE
                     YAEWARRQRDLGRVLDGHKLADEGRTLNSGSSTRLSGYFVVAAPDLDTALEIAKSCPH
                     AAHGGRIEVRAIER"
     gene            complement(1136..1726)
                     /locus_tag="VD788_01825"
     CDS             complement(1136..1726)
                     /locus_tag="VD788_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV75028.1"
                     /translation="MSQSLTEGIGPAAEDDDASLAAVWLRSGADEPFRRLYRRHTPYL
                     YRVAIGLLGGRVDEAQDAVQEAWVRASSRLASFRWRSSLRTWLSGIVVNCCREARKSR
                     SYGRRSLRVAGTESDRAGPGEEASLESIDVLYAVGTLPDGAREVLILHSIFGHSHAEV
                     ASILGISAGTSKSQLSYARARLRERLGHGPSEGSHE"
     gene            complement(1745..2173)
                     /locus_tag="VD788_01830"
     CDS             complement(1745..2173)
                     /locus_tag="VD788_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV75029.1"
                     /translation="MRNATLLATVLLLAALPSSAAELEWKGTLLVQLAVKDLDRSLAF
                     YTEVLGWELISRNDELHWAKLRPIGCDAVVGVGESPRIEGSGTASLNFGVIDVDAARA
                     VLESRGVVFSGETRTIPGVVRLADFADPDGNRFRLAQALE"
     gene            complement(2239..>2554)
                     /locus_tag="VD788_01835"
     CDS             complement(2239..>2554)
                     /locus_tag="VD788_01835"
                     /inference="COORDINATES: protein motif:HMM:NF019469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1646 family protein"
                     /protein_id="HXV75030.1"
                     /translation="LGADPSQPALLFVVTAVLSNLVSNVPATMLLLPTATHPLGGPIL
                     ALASTLAGNMLIVGSIANIIVVDQAARHGLRIGWREHARVGVPVTVGSLLLAGAWLFL
                     VA"
BASE COUNT          400 a          914 c          867 g          373 t
ORIGIN      
        1 acgaggaaga gcgcgacgag cagaaccgtg gcgaccccgg caccggccgc gatccaccgc
       61 ccccgagcgg acaagcgcta ctctttcgga cgacgatagg tgtcgtggca gcccttgcag
      121 ccgttgccga gctggccgag cgccggtccg agcttcgact cttcggtcac ttcggcgagc
      181 gacaccgcgg cgtcgcgcgt ccgctcgaga gcctcgacga agccctcggg tcgggaccag
      241 atctccggct tggcccaggt ttcgatccct cccttctcgc tgccctcggg aaacagcttc
      301 gacgcctggt cgagtagctc cgcgatcttc gtgccgctgc ctcgcacgac ctcggcatcg
      361 aacgaggcct ccttgcggac gatcgacacg acctccttca gcgcggcctg gatcgattcc
      421 atcgtgtgct ggcgctcggc gatgacgtcg tcgctcgacg ggccctcggc cccgacgacg
      481 cacaggaccg acgcgccaag cgccacgacc acgacggccc tctttcgctg cttggacatc
      541 tccccctcct cgttgccgcc gacgaacgga atcggcgaag cgccgattgt acgccaactc
      601 tcggggaggg cacggtcttc agcgctcgat cgcccgtacc tcgatccgcc cgccgtgggc
      661 ggcgtgagga cagctcttcg cgatctcgag cgcggtgtcg aggtccggcg cggcgacgac
      721 gaagtagcca ctcagccggg tggacgatcc cgagttgagc gtgcgccctt cgtcggcgag
      781 cttgtgtcca tcgaggacgc gtccgaggtc ccgctgccgt cgggcccatt cggcgtattc
      841 gcgcacccga ccgttcgcgt cggcgcccgg tcggtcgtcg atcgagaaga gcaagagggc
      901 gtacttgccg tcgggcgctg tcttggcccg gggatcgagc cccgacgcgc cggagatcca
      961 ccccgcgccg aacagcgccg aaccggcgag aaccgccgcg gcccatcgcc agccccttcg
     1021 tcgtcgtcgc gcgtttcgca ccgcgaagac gacccggctc tcgagctccg ccggcgggag
     1081 ccgccgcgcg agttcccgcc gaacgggatc gaggtagcgt tcgacttccg gctcgtcact
     1141 catggcttcc ctcgctcggg ccgtgtccca gccgttcgcg caagcgtgct cgcgcgtagc
     1201 tcagctgact tttcgacgtt cccgcggaga tgccgaggat cgaggcgacc tcggcgtggc
     1261 tgtgaccgaa gatggaatgg agaatcagga cctcgcgcgc gccgtcgggc agcgtgccga
     1321 cggcgtagag cacgtcgatc gactcgaggc tcgcctcctc gccgggtccc gcgcgatccg
     1381 actccgtgcc ggcgacccgc aaggaccgcc gcccgtacga cctcgacttt ctcgcctcgc
     1441 gacagcagtt gacgacgatc ccgctcaacc aggtgcgcag cgagctccgc cagcggaagc
     1501 tcgccagacg cgacgaggcg cggacccacg cctcctgcac cgcgtcctgc gcctcgtcga
     1561 cgcggccgcc gagcaagccg atcgcgacac ggtagaggta cggcgtgtgc cggcggtaga
     1621 gccgacgaaa cggctcgtcg gcgcccgagc gcaaccaaac ggccgcgagg ctcgcgtcgt
     1681 cgtcttcggc ggcggggccg attccttcgg tcaacgactg gctcaccagg gctccgccgg
     1741 tcgtctattc gagggcctgg gcgagacgaa accggttgcc atcaggatcg gcgaagtcgg
     1801 cgagccggac aacccccgga atcgtccgcg tctcgcccga gaagacgact ccgcgcgatt
     1861 cgagcacggc gcgcgccgca tcgacgtcga tcacgccgaa gttgagcgag gcggtgccgc
     1921 tgccctcgat ccgcggggac tcgccgacgc cgaccacggc gtcgcagccg atcggccgga
     1981 gcttcgccca gtgcagttca tcgtttcgcg agatcagctc ccagcccagg acctcggtgt
     2041 agaacgcgag cgatcggtcg aggtccttca ccgccagttg gacgagcagc gtccctttcc
     2101 attcgagctc ggcggcgctc gacggcaggg cggcgagcag caggacggtg gccagcaggg
     2161 tggcgttgcg catgattcac ctccgcgaga tgagtggcgc gaaccggtcg ggtggttgga
     2221 acgggacccg gccggaggtc acgcgacgag gaacaaccag gccccggcca gcagcagcga
     2281 gcccacggtc accgggacgc cgacccgcgc atgctcgcgc cagccgatgc gcaggccgtg
     2341 gcgcgccgcc tggtcgacga cgatgatgtt ggcgatgctg ccgacgatca gcatgttccc
     2401 ggccagcgtg ctggccagcg cgaggatcgg accaccgagc ggatgggtcg cggtgggaag
     2461 caacagcatc gtggccggta cgttcgacac caggttcgac aggaccgcgg tgacgacgaa
     2521 cagcagggcc ggctgcgacg ggtcggcgcc gagc
//
LOCUS       DASPGN010000062         7289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_948533, whole genome shotgun sequence.
ACCESSION   DASPGN010000062 DASPGN010000000
VERSION     DASPGN010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948533"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="VD788_01840"
     CDS             complement(<1..955)
                     /locus_tag="VD788_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75031.1"
                     /translation="MRSFGLHVVRCLAIVAAATAAVAQAPPGEYGNLDLIQITHEQIA
                     SGDLTLNQIRDNGMRVFTIPFNKLDGYGDGPMNLDDTVSPGGRPTLQGNGTFLRVNGL
                     DAQTCLECHSLTSTATVPPTLGIGGVGGSSTNAMIMPTAMDPADLEDLDSAAGFNGRF
                     ANPPFLFGAGGVELLGLEMTRDLQQLKQWALDNPGTTVPLVTKGISFGSIVADAFGEI
                     DYSAVEGVEHDLIVRPFGRKGEFPTTRDFDIAAMQFHFGMQPVEVVGEGSDADGDGVV
                     DEILVGELSALHVFVSSMPRPEKDKPTGQTTRGFSRFQEIGC"
     gene            1195..2373
                     /locus_tag="VD788_01845"
     CDS             1195..2373
                     /locus_tag="VD788_01845"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HXV75032.1"
                     /translation="MIAGVVKETFPGERRVAVTPHVLPQLAKAGVELLVESGAGLDAG
                     FPDSVYGEKGAKVVAARSAVFERADAILQVRGYGANPERGAADLESMRPGLVLIGAFE
                     PLTAGEQVKAVAERGVTLFALELVPRITRAQSMDILSSMATIAGYKAVLLAAQQLPKM
                     FPMMMTAAGTVKPAKVFVIGAGVAGLQAIASSRRLGAVVEAYDLRPAVREQVESLGGK
                     FVEMELDTRAAEGAGGYARAMDEEFYRKQRELMTRVVAEQDVVISTAAVPGRQAPVLI
                     TEEMVRGMQPGSVIVDLAAERGGNCELTRAGEIVETHGVTIIGPVNLPATVPYHASQM
                     FAKNITTFLLNMVKDGRLQIDKDDEIVRESLVVRDGEIANPRVREAVGLAAAASGSES
                     "
     gene            2408..2689
                     /locus_tag="VD788_01850"
     CDS             2408..2689
                     /locus_tag="VD788_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HXV75033.1"
                     /translation="MEVFVGVLTVFVLAIFVGFEIITKVPPTLHTPLMSGSNAISGIT
                     IVGALLLAGQIGNSPVAKVLAAVAVFFAAINVVGGFLVTHRMLEMFRKK"
     gene            2699..4294
                     /locus_tag="VD788_01855"
     CDS             2699..4294
                     /locus_tag="VD788_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HXV75034.1"
                     /translation="MIPPQLRDVVYLVAAVLFILDLKWMAHPRTAVRGNLAGVAGMAL
                     AIFATLASRPLEWPYILVATLAGSLVGVVSASRVKMTAMPEMVGLFNGFGGGASALVA
                     GSALIATFSAVAGGTGMAQQAGDLQMRIATVASGIIGSVTFFGSYVAFGKLAEFLAVR
                     WKLHPWQRVIKYGFSLAVVGGGIAFALGVAVIPHGLTSRPGLLSLLVIVCAGAIGAVL
                     LLGKDRFPGIDALKVITAVASVGVGVLVVLRPEDFVMFTLVVALAGLLGVLLTFSIGG
                     ADMPVVIALLNSYSGLAAAATGFVINNTVLIISGSLVGASGIILTGIMCRAMNRSLAN
                     VLFATLGPTADTPDAEEVYKTVKATSAEEIAMLLDGAQRVVVVPGYGMAVAQAQHAVR
                     GLAQLLEARGASVEYAIHPVAGRMPGHMNVLLAEANVEYEKLREMDDINPEMEQIDVC
                     LVLGANDVVNPVARTDPNSPIAGMPIIDVDKARTVVVIKRSLSPGFAGIPNPLFARDN
                     TLMFFADGKKAVQDIVAAVKEAA"
     gene            complement(4436..5497)
                     /locus_tag="VD788_01860"
     CDS             complement(4436..5497)
                     /locus_tag="VD788_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HXV75035.1"
                     /translation="MEQLLPEGWFAGLPQWALLLITAASAGVLIKGADWLVEGAAGIA
                     YHFGISQVIVGATVVSLGTTSPEAAVSVVAAWSGEPGLALGNAIGSVIADSGLIFGLG
                     CLLVVLPADRFVLQRQGWVQFGSGSLLCGLCYATWAWAGDDATLPRWVGGMLLALLIV
                     YLWVSVCWSRRHPQGEPFQVHEKEDVVAGLRRPVPMLIGVGLAGIVLVLLASRTMVLS
                     VTELAEEHWHVPKLVIAATLVALGTSLPELVVGMTSILKGHNELLVGNVIGADILNVL
                     FVVGAASLAAPLPLLEHGSSLPSILFLLHLPTMLLILVLFRLFIRSACHHRRFRRWHG
                     VPLVLLYVAYTVAQYVLAR"
     gene            complement(5500..6750)
                     /locus_tag="VD788_01865"
     CDS             complement(5500..6750)
                     /locus_tag="VD788_01865"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HXV75036.1"
                     /translation="MTIFALIAILLTVTALLSWVNARLIHLPPTIGVMTAALCLSLLL
                     IALGRVGLGVDRWAEALFAHVDFDTVLMQGMLSFLLFAGALHVDLGALLERKWTIASL
                     ATVGVMISTFLVGTTFFYVLQAFGDPIPYTWALLFGALISPTDPVAVLGILKNANAPH
                     SLEIHIVGESLFNDGVGVVVYAVLLGFVVDPEPFSLPATLGLLAEEALGGAMVGAALG
                     WLAYRLLREIDDYSVEILITLALVSGGYALAGAIHTSGPIAMVVAGLFIGNHGRAFGM
                     SERTREHLDAFWEMLDELLNALLFVMIGLELLVLDVERVYLAIGLVAIAIVLLARTVS
                     VAVPISAIRAFRPIEAQTIRVLSWAGIRGGISIALALGLPASPARDLILVVTYTVVVF
                     SILVQGLTVGRVVRWVTANTRTGA"
     gene            complement(6747..>7289)
                     /locus_tag="VD788_01870"
     CDS             complement(6747..>7289)
                     /locus_tag="VD788_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HXV75037.1"
                     /translation="IERSVDDRTILASNTSSLSIDRIADATRLPRRVIGLHFFNPVHL
                     MPLLEIVRGSRTADEIVERAIELGTTIGKQPIVVRDSPGFASSRLGLALGLEAMRMLE
                     QGVASADDIDKAMTLGYRHPMGPLRLTDLVGLDVRLAIAEYLHRELGSDAFRPPEILR
                     RMVARGDLGKKSGRGFYEWE"
BASE COUNT         1251 a         2408 c         2504 g         1126 t
ORIGIN      
        1 cgcagccgat ctcctggaag cggctgaagc cccgcgtggt ctggccggtc ggcttgtcct
       61 tctcggggcg gggcatgctc gacacgaaga cgtgcagggc cgaaagctcg ccgacgagga
      121 tctcgtcgac cacgccgtcg ccgtccgcgt cgctgccctc gccgacgacc tcgaccggct
      181 gcatgccgaa gtgaaactgc atcgccgcga tgtcgaaatc acgcgtcgtc gggaactcgc
      241 ccttgcgacc gaacggccgg acgatcaggt cgtgctcgac accctcgacc gccgagtagt
      301 cgatctcgcc gaatgcgtcg gcgacgatcg agccgaagct gatgcccttg gtcacgagcg
      361 gcaccgtcgt tccggggttg tcgagcgccc actgcttcag ctgctgcaga tcccgcgtca
      421 tctcgagacc gagcagctcg acgccgccgg caccgaacag gaacggcggg ttggcgaacc
      481 gtccgttgaa gcccgccgcg ctgtcgagat cttcgaggtc ggcggggtcc atcgccgtcg
      541 gcatgatcat cgcgttggtc gacgacccgc cgacgccgcc gatgccgagc gtcggtggga
      601 cggtggcggt gctggtcagc gagtggcact cgaggcaggt ctgcgcgtcc aggccgttga
      661 cgcgcaagaa ggtcccgttg ccctgcagcg tcgggcgccc gcccgggctg accgtgtcgt
      721 cgaggttcat cgggccgtcg ccgtaaccgt ccagcttgtt gaacgggatg gtgaagaccc
      781 gcatcccgtt gtcgcggatc tggttcagcg tgaggtcgcc cgaggcgatc tgctcgtgcg
      841 tgatctggat cagatcgaga ttgccgtatt ctcccggcgg agcttgggcg acggccgcgg
      901 tcgcggcggc gacgatcgcc aggcagcgga cgacgtgaag cccgaatgaa cgcatcggaa
      961 tcccccctcc aggccaaggc ccgggcgcgc gcgccacggg cgaagatacc gtcaacccgg
     1021 agcggaagca ctcggtttcg ggcgaacgcg acggcggggc gaggtaagcg gccggcggag
     1081 tccagaatca ggtgtatcgt cggctcggga ttctgtagaa ttcgtctctt ttgagcgggg
     1141 cgctgcctcg tcgtcgcagc ggtgcccgga gcacggaaag caggcaggaa tacgatgatc
     1201 gctggagtcg tcaaagagac cttccccggc gagcggcggg tcgccgtcac cccgcacgtg
     1261 ctgccccagc tcgccaaggc cggggtcgag ctcctcgtcg agtcgggtgc cggtctcgac
     1321 gccgggtttc ccgactcggt ctacggcgaa aaaggagcca aagtcgtcgc cgcgcggagc
     1381 gcggtcttcg aacgggccga cgccatcctc caggttcgcg gctacggtgc gaaccccgag
     1441 cgcggtgcgg cggacctcga atcgatgcgg ccgggcctgg tgctgatcgg ggcgttcgag
     1501 ccgttgaccg cgggtgagca ggtgaaggcg gtggcggagc gcggcgtcac gctgttcgcg
     1561 ctcgagctcg tgccgcgcat cacgcgggcg cagagcatgg acatcctgtc gtcgatggcg
     1621 acgatcgccg gctacaaggc ggtgctgctc gccgcgcagc agttgccgaa gatgttcccg
     1681 atgatgatga ccgccgccgg cacggtgaaa ccggcgaagg tcttcgtcat cggcgccggt
     1741 gtcgccggcc tgcaggcgat cgccagctcg cgccgactcg gtgcggtggt cgaggcctac
     1801 gatctgcggc cggcggtgcg cgagcaggtc gagagcttgg gcgggaagtt cgtcgagatg
     1861 gagctcgaca cgcgcgcggc cgagggcgcc ggtggctacg cccgcgcgat ggacgaggag
     1921 ttctaccgca aacaacgcga gctgatgacg cgggtggtag cggagcagga cgtcgtcatc
     1981 tcgacggcgg ccgtgccggg tcggcaggcg cccgtgctga tcaccgagga gatggtgcgc
     2041 ggcatgcagc ccggctcggt gatcgtcgac ctggcggccg agcgcggcgg caactgcgag
     2101 ctgactcgcg ccggcgagat cgtcgagacc cacggtgtga cgatcatcgg cccggtcaac
     2161 ctgcccgcga ccgtgccgta ccacgcgagc cagatgttcg ccaagaacat cacgactttc
     2221 ctgctcaaca tggtcaagga cggtcgactg caaatcgaca aggacgacga gatcgttcga
     2281 gagtcgctcg tggtgcggga cggcgagatc gccaacccgc gggtgcgcga ggcggtcggg
     2341 ctggccgccg cggccagcgg gtccgagtcc tgagcgtcgt tctttatccg gtagaggact
     2401 gaacgggatg gaagtattcg tcggtgtcct gacggtcttc gtgctggcga ttttcgtcgg
     2461 cttcgagatc atcaccaagg ttcccccgac gctgcacacg ccgctgatgt ccggatcgaa
     2521 cgcgatctcc ggcatcacga tcgtcggcgc gttgttgctc gcggggcaga tcggcaactc
     2581 gccggtggcg aaggtgctgg cggcagttgc cgtcttcttc gcggcgatca acgtcgtcgg
     2641 cgggtttctc gtgacccacc gcatgctcga gatgttccgc aagaagtagg gtgcggcgat
     2701 gattcccccg cagctgagag acgtcgtcta cctcgtggcg gccgtgctgt tcatcctcga
     2761 cctgaagtgg atggcgcacc cgcgaaccgc cgttcgcggc aatctcgccg gtgtcgccgg
     2821 tatggcgctc gcgatcttcg cgaccttggc gagccggccg ttggaatggc cgtacatcct
     2881 cgtcgcgacg ctggccgggt cgttggtcgg cgtcgtgtcg gcgtcgcggg tcaagatgac
     2941 cgcgatgccg gagatggtcg ggctgttcaa cgggttcggc ggcggtgcct cggcgttggt
     3001 cgcaggctcc gcgttgatcg cgaccttcag tgcggtcgcc ggcggaacgg gcatggcgca
     3061 acaggccggc gacctgcaga tgcgcatcgc gaccgtcgct tcgggcatca tcggttcggt
     3121 cacgttcttc ggcagctacg tggcgttcgg caagctggcc gagttcctcg ccgtccgctg
     3181 gaagctccac ccctggcagc gggtgatcaa gtacggcttc tcgctggcgg tggtcggggg
     3241 cgggatcgcg tttgcgctgg gcgttgccgt gatcccccac gggctgacca gccgacccgg
     3301 gctgctctcg ctgctggtca tcgtttgtgc gggcgcgatc ggcgccgtgc tgttgctcgg
     3361 aaaagaccgc ttccccggga tcgacgcgct gaaggtgatc accgccgtcg cgtcggtcgg
     3421 cgtcggcgtg ctggtcgtgc tgcgtcccga ggactttgtc atgttcacct tggtcgtcgc
     3481 actcgccgga ctgctcggcg tcctgctgac gttcagtatc ggtggcgccg acatgccggt
     3541 cgtcatcgcg ctgttgaact cgtattcggg gctggccgcc gcggccaccg gcttcgtcat
     3601 caacaacacg gtgctgatca tctccggctc gctcgtcggc gcgtcgggaa tcatcctcac
     3661 cggcatcatg tgccgtgcga tgaaccgctc gctggccaac gtgttgttcg cgacgctggg
     3721 cccgacggcc gacactcccg acgccgaaga ggtctacaag acggtgaagg cgacctcggc
     3781 ggaggagatc gcgatgctgc tcgacggcgc ccagcgcgta gtcgtcgttc cgggatacgg
     3841 catggcggtc gcccaggccc agcacgcggt gcggggtctc gcgcaactgc tcgaagcgcg
     3901 aggcgcctcc gtcgagtacg cgatccaccc ggtggccggg cgtatgcccg ggcacatgaa
     3961 cgtgctgctg gccgaggcca acgtcgagta cgagaagctg cgggagatgg acgacatcaa
     4021 ccccgaaatg gagcagatcg acgtctgcct cgtgctcggc gcgaacgacg tggtcaaccc
     4081 ggtggcccgc accgatccga acagcccgat cgccggcatg ccgatcatcg acgtcgacaa
     4141 ggcgcgaacc gtcgtcgtga tcaagcgcag tctcagcccg ggcttcgccg gcatacccaa
     4201 cccgctgttc gcgcgggaca acacgttgat gttcttcgcc gacggcaaga aggccgtaca
     4261 ggatattgtt gcggcggtca aggaagcggc ttagacttac cgtgttctgt ttcgattctt
     4321 cctgcgtcga cgcgccctgc caaactacct cccccggcgg ggcgcttttt tttgcctgcc
     4381 gcgcgctcgc tgcctacgga cggtggcgcg acggaatgat ggctccggga cagacctacc
     4441 gcgccaggac gtactgcgcg acggtgtagg cgacgtagag tagaaccagc ggaacgccgt
     4501 gccagcgtcg gaagcgccgg tggtgacagg ccgaccggat gaacaggcgg aacaacacca
     4561 ggatcaacaa catcgtcggc agatgcagca ggaacaggat gctcggcagc gagctgccgt
     4621 gctcgagcag cggtaacggc gccgcgagtg acgccgcccc gacgacgaac agcacgttga
     4681 ggatgtcggc gccgatcacg ttgccgacca acagctcgtt gtggcccttg aggatgctgg
     4741 tcatgccgac gaccagttcg ggcagcgagg tccccagcgc gacgagcgtc gccgcgatga
     4801 cgagcttcgg aacgtgccaa tgctcctcgg ccagctcggt gacggacagg accatcgtgc
     4861 ggctggcgag cagcaccaac acgatgccgg cgaggccgac accgatcagc atcggcacgg
     4921 gtcgccgcag cccggcgacc acgtcctcct tctcgtggac ctggaacggc tcgccctgcg
     4981 gatgccggcg gctccagcac accgagaccc acaggtagac gatcaacagc gcgagcagca
     5041 tgcccccgac ccagcgcggc agggtggcgt cgtctccggc ccacgcccac gtcgcgtagc
     5101 acagaccgca caacaacgat ccggagccga actggaccca accctggcgc tgcagcacga
     5161 agcgatcggc cggcagcacg accaacagac atcccaggcc gaagatcaac ccgctgtccg
     5221 cgatcaccga gccgatcgcg tttcccaacg cgaggccggg ctcgcccgac caggcggcga
     5281 cgaccgacac cgccgcctcg gggctcgtcg ttccgagcga gacgaccgtg gcgccgacaa
     5341 tcacctggga gatgccgaag tggtaggcga tgccggcggc gccctcgacc aaccaatcgg
     5401 cgcccttgat cagcacgccg gccgacgcgg cggtgatcaa cagcagcgcc cactgcggca
     5461 gcccggcgaa ccatccctcg ggaagcagct gctccacgtc taggcgcctg tccgagtatt
     5521 cgcggtgacc cagcgcacga cgcggcccac ggtcaagccc tggaccagga tcgagaagac
     5581 cacgacggtg taggtgacga cgaggatcag gtcgcgcgcg ggagaggccg gcagccccag
     5641 cgccagcgcg atcgagatac cgccacgaat gccggcccag ctgagcacgc ggatcgtctg
     5701 cgcttcgatc ggccggaacg cccgtatcgc gctgatcggc accgcgacgc tgacggtccg
     5761 cgccagcagc acgatcgcga tcgcgaccag accgatggcg agatagaccc gttccacgtc
     5821 gagcaccagc agctccaaac cgatcatgac gaacagcagc gcgttgagga gctcgtcgag
     5881 catctcccag aaggcatcga gatgctccct cgtccgctcg gacatgccga acgcgcggcc
     5941 atggttaccg atgaacaagc cggcgacgac catcgcgatc ggccccgacg tgtggatcgc
     6001 gccggccaac gcgtagccgc ccgaaaccag cgccagcgtg atcaggatct cgaccgagta
     6061 gtcatcgatc tcgcgcagca gccggtaggc caaccagccg agcgcggcgc cgaccatcgc
     6121 gccaccgagc gcctcttcgg cgagcagtcc gagtgtcgcc ggcaacgaaa acggctcggg
     6181 gtcgacgacg aagccgagca gcaccgcgta gacgacgacg ccgacgccgt cgttgaacag
     6241 cgactcgccg acgatgtgga tctcgaggct gtgcggagcg ttcgcgttct tcaggatgcc
     6301 gagcacggcg accgggtcgg ttggcgagat caacgcgccg aacagcagcg cccaggtgta
     6361 agggatcgga tcgccgaacg cctgcagcac gtagaagaac gtggtcccga ccaagaaagt
     6421 cgagatcatg acgccgacgg tcgcgagcga cgcgatcgtc cacttgcgct cgagcaacgc
     6481 gccgaggtcg acgtgcaacg caccggcgaa caacaggaag ctgagcatgc cttgcatcaa
     6541 caccgtgtcg aagtcgacgt gggcgaacaa cgcctcggcc cagcgatcga cgccgagacc
     6601 gacgcggccg agggcgatca acagcagcga aaggcagagc gccgcggtca tcaccccgat
     6661 cgtcggcggc aggtggatca agcgagcgtt gacccagctg agcaacgcgg tgaccgtcaa
     6721 caggatcgcg atcagcgcga agatcgtcat tcccattcgt agaagccgcg accgctcttc
     6781 ttgcccagat cgccacgcgc gaccatccgg cgcaggatct ccggcggccg gaaggcgtcg
     6841 gagccgagct cgcggtggag gtactcggcg atcgccagcc gtacatcgag gccgaccaga
     6901 tcggtcaggc gcaacggtcc catcgggtgg cggtagccga gggtcatcgc cttgtcgatg
     6961 tcgtcggccg aggccacgcc ctgctcgagc atccgcatcg cctcgagccc gagcgccagg
     7021 ccgagccggc tcgaggcgaa gccgggcgag tcgcggacga cgatcggctg cttgccgatc
     7081 gtcgtgccga gctcgatcgc ccgctcgacg atctcgtccg ccgtccggct gccgcgaacg
     7141 atctcgagca gcggcatcag gtgcaccggg ttgaagaagt gcagaccgat gacacgtcgc
     7201 ggcagccggg tcgcgtcggc gatccggtcg atcgacaacg acgacgtgtt gctggccagg
     7261 atcgtccgat cgtcgaccga gcgctcgat
//
LOCUS       DASPGN010000063         3978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_831833, whole genome shotgun sequence.
ACCESSION   DASPGN010000063 DASPGN010000000
VERSION     DASPGN010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831833"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="VD788_01875"
     CDS             <1..1536
                     /locus_tag="VD788_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HXV75038.1"
                     /translation="LDELSSMQLDLASLSACASPDACGIAFTAAGGGDEAGWSLSYAG
                     DIDHDGHDDLLIGAPGASPAGRAQAGAVYLIYGPLEAGTIDLSEVGDPSMPGIETRGL
                     RFDGENPGDRAGEAVSWWEDVNADGVDDLLIGAPGATLLDPTGTPVAGAGIVYAIHGG
                     TRNLDDSATPGVIELSRVANTEPDEVTGVVFLGSAEHRHAGASVTGAADTDGDEEPDI
                     YIGGDDEVWFISGLDPKTTSGSSPMDRDRGGVGLSRMIRVLGPGSVADVFPATAFVAG
                     SDGDLGPLTVGPAGDIDGDGIDDVVIGAAEADPAGRVDAGKAYLVLGSRVRREAVVEL
                     SEVGRTIPGLVIDGSEAGDALGRSVGGGFDVNGDGIDDGLVGAPLADSRPDTPPDAGE
                     AYVISPVAPGEVPGLQLSRGPVATELEWVPPHRALRYNVYRGTLADLRAAGVVRTSDV
                     PGTFECALNTDADGDGLPDTVDSQTPSPGEGYLYLVSGRNLSGEGPLGPLPAARINDG
                     QCP"
     gene            1571..>3978
                     /locus_tag="VD788_01880"
     CDS             1571..>3978
                     /locus_tag="VD788_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV75039.1"
                     /translation="MKLEPERWRRIEPLLAEALERDAAERSAWIEAACGEDVGLRAEL
                     RALVAAGDRGDGPLDRSAGEVVRELLEATLGFDDAGDAAVAFTGPKRIADYEIVRELG
                     HGGMGTVFEADESRMNRRVALKVLSRHHATSAKAGLRFEREAWIAGKLDHPHLVKVFD
                     RGVWQELAFYSMELVEGGSLHDVIDNMKRWGRDDRIGLEFGTREYVDWSITQIITAAR
                     ALDYAHRHHVIHRDVKPMNLLWSREHATVKVADFGLAIDSDVSRLTTTGKLVGTLAYM
                     APEQLLGRTDAIGAGTDVYALGATLFQLLTLELPYGGESQQLYMNAVLTSEARRPSKL
                     NRRVSRDLEAVIGRALEKNPRDRYASAAAFADDLENVLRFRPVLAQPPTWLNTLGKWV
                     RRRPVHAALVAVLLVVVPALGVLGLRARQHARVQRQLQVEELWTGARWLFLNRRDREF
                     LERTERILALDPGHLEARRARAGAWLNLALETDGDERRTTEERALDEVLRIQQLEPER
                     AWPHALEAIVLERLGRDEAAERARQAARTLASDPPHPDDLMFRARQIRGTGDLDETLR
                     LLDELLARAPSNLAAIQFRGHVLAESGRLEPAIADFRFGTVLAPDDFYGYYQLGRLYV
                     EAGEVERGEEQLRQALELARDDELFLENLSDEYLQWGRRALADGQTRRAADCFIAAEH
                     HARRSIELGPTLVWAHVNLGASLMERYRLAEPQVPELANEAVEHYARASALLREPGTD
                     RDVSMHAAVLVNTCDAMIQLGQLERALESCHEVVERFPDDPLGHYNLAGVYALLGRTD
                     EALRS"
BASE COUNT          589 a         1396 c         1449 g          544 t
ORIGIN      
        1 ctcgacgagc tgtcgtcgat gcagctcgac ctggcgagcc tgtccgcgtg cgcatcgccc
       61 gacgcctgcg ggatcgcctt caccgcggcc ggtggcggcg acgaagccgg ctggtcgctg
      121 tcctacgccg gcgacatcga ccacgacggc cacgacgacc tgctgatcgg agcgccgggc
      181 gcgagccccg cagggcgcgc gcaggccggg gccgtgtatc tgatctacgg cccgctcgag
      241 gccgggacga tcgacctgtc cgaggtcggt gatccatcga tgccgggcat cgagacacgc
      301 ggtctgcgct tcgacggtga gaatccgggc gaccgcgcgg gcgaggcggt ctcgtggtgg
      361 gaggacgtca acgccgacgg cgtcgacgac ctgttgatcg gcgcgccggg cgccacgctg
      421 ctcgatccga cggggacgcc cgtcgccggc gccgggatcg tctacgcgat ccacggcggc
      481 acacgcaacc tcgacgactc ggcgaccccc ggagtgatcg agctgtcgcg cgtggccaac
      541 accgagcccg acgaggtgac cggggtcgtc ttcctcggtt cggcggagca ccgccacgcc
      601 ggggcttccg tcaccggcgc cgccgacacc gatggcgacg aggagccgga catttacatc
      661 ggcggcgacg acgaggtttg gttcatctcg gggctcgatc ccaagaccac gtccggcagc
      721 tcgccgatgg accgcgatcg gggcggcgtg ggcctgtcgc ggatgatccg ggtgctcggc
      781 ccaggaagcg tcgccgacgt cttcccggcc accgcgttcg tcgccggcag cgacggtgac
      841 ctgggtccgc tcacggtcgg tcccgccggc gacatcgacg gcgacggcat cgatgacgtc
      901 gtgatcggcg ccgccgaagc cgatccggcc ggccgggtcg acgcgggcaa ggcctacctc
      961 gtgctcggca gccgcgtccg gcgcgaggcg gtcgtcgagc tgtcggaggt cggccggacg
     1021 ataccgggtc tcgtgatcga cgggtcggag gccggcgacg ctctgggtcg ctcggtcggc
     1081 ggcggcttcg acgtcaacgg cgacgggatc gacgacggcc tggtcggtgc tccgctcgcc
     1141 gactcgcggc cggacacccc gcccgacgcg ggcgaagcct acgtcatctc gcccgtggcg
     1201 cccggcgagg tgcccggact tcagctgtcc cgaggcccgg tcgcgacgga gctcgagtgg
     1261 gtgccgcccc accgcgcgtt gcgctacaac gtctaccgcg gcacgctcgc cgacctgcgg
     1321 gcggcgggcg tcgtccgcac ctctgacgtc ccgggcacct tcgagtgcgc gttgaacacg
     1381 gatgccgacg gggacggtct gcccgacacc gtcgactccc agaccccgtc gccgggtgag
     1441 ggttacctgt atctcgtcag cggccggaac ctgagcggcg aaggtccgtt ggggccgctg
     1501 ccggccgcac gcatcaacga cggccaatgt ccgtgagcat cgtcacgcct cgccccggga
     1561 gacccgttcc gtgaagctcg agcccgagcg ctggcgcagg atcgagccat tgctcgccga
     1621 agcgctcgag cgcgacgccg ccgaacgctc ggcctggatc gaggccgctt gtggcgaaga
     1681 cgtcgggctg cgcgcggagc tccgggcgct cgtggccgcc ggcgaccgtg gcgacgggcc
     1741 gctcgaccgc agcgccggcg aggtggtgcg cgaactgctc gaggccaccc tgggcttcga
     1801 cgacgccgga gacgcagcgg tggcgttcac cggtcccaaa cgtatcgccg actacgagat
     1861 cgtgcgcgag ctcggccacg ggggcatggg caccgtgttc gaggccgacg agagccggat
     1921 gaaccggcgc gtcgcgctca aggtgctctc gcggcatcac gcgacatcgg cgaaggcggg
     1981 cttgcgcttc gagcgcgaag cctggatcgc cggcaagctg gaccatcctc acctggtcaa
     2041 ggtcttcgat cggggcgtgt ggcaggagct cgcgttctac tcgatggagc tcgtcgaggg
     2101 cggttcgctg cacgatgtga tcgacaacat gaagcgctgg ggacgggacg accgcatcgg
     2161 cctcgaattc ggaacgcgcg agtacgtcga ctggagcatc acacagatca tcaccgcggc
     2221 ccgcgcgctg gactacgcgc accgccacca cgtgatccac cgcgacgtga agccgatgaa
     2281 cctgctgtgg agccgcgagc acgcgacggt caaggtcgcc gatttcggtc tggcgatcga
     2341 cagcgacgtc agccgactga cgacgaccgg gaagctggtg ggcacgctgg cctacatggc
     2401 cccggagcag ctcctgggcc ggaccgacgc gatcggcgcg ggcacggacg tctacgccct
     2461 gggggccacg ctgttccagc tcttgacgct cgagctgccc tacggcggcg agtcccagca
     2521 gctgtacatg aacgccgtgc tgacgtccga ggcgcggcga ccgagcaagc tcaatcggcg
     2581 cgtcagccgc gacctcgagg cggtgatcgg cagggcgctg gagaagaacc cgcgcgaccg
     2641 ctacgcctcg gccgcggcgt tcgccgacga cctggagaac gtgctgcgct tccgaccggt
     2701 cctggctcag ccgccgacct ggctgaacac gctgggcaag tgggttcgac gcaggccggt
     2761 gcacgcggcg ctcgtcgcgg tgctcctcgt cgtcgtaccg gcactgggag tcctcggcct
     2821 gcgcgcgcgc cagcacgcgc gagtgcagcg ccaactgcag gtcgaagagc tgtggaccgg
     2881 cgcgcggtgg ctgttcctca atcgacgcga ccgggagttc ctcgagcgca ccgagcgcat
     2941 cctcgcgctc gatccgggcc acctcgaggc gcgtcgagcg cgcgccgggg cctggctgaa
     3001 cctcgcgctc gagacggacg gcgacgagcg gcgcacgacg gaggagcgcg cgctcgacga
     3061 agtgctgcgc atccagcagc tcgagccgga gcgcgcgtgg ccccacgcgc tcgaagcgat
     3121 cgtcctggaa cgactgggcc gggacgaggc ggccgagcgt gcacgccagg cggcgcggac
     3181 cctcgcctcg gacccgccgc atcccgacga cctgatgttc cgcgcgcgcc agatccgcgg
     3241 cacaggcgac ctggacgaga cgttacggct gctggacgag ctcttggccc gagcgccgag
     3301 caatctcgcg gcgatccagt tccggggcca cgtgctcgcg gagagcggcc ggctcgagcc
     3361 ggcgatcgcc gacttccgct tcggcacggt gctggcaccg gacgacttct acggctacta
     3421 ccagctcggc cggctgtatg tcgaggccgg cgaggtcgag cgtggcgagg agcagctgcg
     3481 ccaggcgctg gaattggcgc gcgacgacga gctgttcctc gagaacctgt cggacgagta
     3541 cctgcagtgg ggccgtcgcg cgctcgccga cggtcagacc cggcgcgccg ccgactgttt
     3601 catcgcggcg gagcaccacg cgcggcgcag catcgagctc gggccaacgc tcgtctgggc
     3661 gcacgtcaat ctcggcgcga gcctgatgga gcgctaccgc ctcgccgagc cgcaagtgcc
     3721 ggagctggcg aacgaagccg tcgagcacta cgcgcgagcg agcgcgctgt tgcgagagcc
     3781 ggggaccgat cgcgacgtct cgatgcacgc cgcggtgctg gtcaacacgt gcgacgcgat
     3841 gatccagctc ggacagctcg agcgcgcgct cgagagctgt cacgaagtgg tcgagcgctt
     3901 tcccgacgac ccgctgggcc actacaacct ggccggggtc tacgcgctgc tcggccggac
     3961 cgacgaggcg ctgcggtc
//
LOCUS       DASPGN010000064         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_831938, whole genome shotgun sequence.
ACCESSION   DASPGN010000064 DASPGN010000000
VERSION     DASPGN010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831938"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2037)
                     /locus_tag="VD788_01885"
     CDS             complement(<1..>2037)
                     /locus_tag="VD788_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75040.1"
                     /translation="NRYGRVRGGVFPRSVHEQNEVSVPLPAVGLTIDGNPVSSDVAGR
                     FDYGGGAAVSGLDGPWFATDCQGCLDPTQAHFQFDLGAGYVDFGIGGVDQFGNGFSTR
                     AERNTFYHLNQVRRIALKWLPGLPWLFTSVTSVANIDRACNAFYDGNVNFFRASGICN
                     NTGEIADVITHEWGHGIDEFTLLGDLATGEGTADVVALHSSHSPVIGEGFRFTGEPVR
                     NLDPAGPRGLLTTTNLGAQCTQTGYLGPLGYEAHCEGEIYGQTAWELSQALVAKYGHH
                     TGWRVSERLFYTSLPDAGGYLPSDASPIYAAYLNADDDDGNLANGTPNAAEIFDAFDR
                     HGIAGAAVPSSTACTPPAQPAVSVVPTCGGFDLSWAPVAGAEQYRIFRGELLADQALF
                     PVVTLSGDRTSHVDLEVAEELDYWYVVTAVDSAGCESTIEAPVAVRLPERPRLSIVAA
                     IVDDTPRGNRSGFADPGEEVDLVVDLQNLGSAGASSVGGTITPLAAGIELLDAEAAWP
                     DLAPGATAANLDTLRFRADSACGSLLAFEFVPYSAAGCAPDPSYFTIELGDGGVCDPT
                     PACFVAPTFAGLDGVAPGGCGESWLTWPVGQSHCENATLAYNVYRSTTAGFVPSAATL
                     VASGVAATTFQDTLLVPDQTYHYIVRADDSRSGEDANLAAQSIVAPGPPDVTAP"
BASE COUNT          316 a          689 c          744 g          288 t
ORIGIN      
        1 ccggggcggt gacgtccggc ggccccggcg cgacgatcga ctgcgcggcg aggttcgcgt
       61 cctcgcccga gcgcgaatcg tcggcgcgaa cgatgtagtg ataggtctgg tccggcacga
      121 gcagcgtgtc ctggaaggtc gtggcggcca cgccggaggc gacgagcgtc gccgccgacg
      181 gaacgaagcc ggccgtcgtg ctgcgataga cgttgtaggc gagggtcgcg ttctcgcagt
      241 gagattgacc gacaggccag gtgagccagc tctctccgca gccgccgggc gcgacgccgt
      301 cgagcccggc gaaggtcggc gcgacgaaac aggcgggcgt cgggtcgcac acgccgccgt
      361 caccgagctc gatggtgaag tacgacgggt ccggcgcaca gccggccgcg ctgtacggca
      421 cgaactcgaa ggccagcagg ctcccgcagg cggaatcggc acgaaagcgc agcgtgtcga
      481 ggttggcggc ggtcgcgccc ggagccaggt cgggccaggc cgcctccgcg tccagcagct
      541 cgataccggc ggcgagcggc gtgatcgtcc cgccgaccga cgaggcaccg gcactaccca
      601 gattctgcag gtcgacgacc aggtcgactt cctcgcccgg gtcggcgaaa ccggagcggt
      661 tcccgcgcgg cgtgtcgtcg acgatcgcgg cgacgatcga cagcctcggc cgctcgggga
      721 ggcgcaccgc gaccggagct tcgatcgtcg actcgcatcc ggcggagtcg accgccgtca
      781 ccacgtacca gtagtccagc tcctcggcga cctcgaggtc gacgtggctc gtgcggtcgc
      841 cggagagcgt gacgacggga aacagcgcct ggtccgccag cagttcgccg cggaagatcc
      901 ggtactgctc cgcgccggcg accggcgccc agctcaggtc gaagccgccg caggtcggca
      961 ccacgctgac cgccggttgg gccggcggcg tgcaggcggt cgacgacggc accgccgcac
     1021 cggcgatgcc gtgtcgatcg aaggcgtcga agatctcggc ggcgttcggc gttccgttgg
     1081 ccaggttgcc gtcgtcgtcg tccgcgttga ggtaggccgc gtagatcggc gacgcatcgc
     1141 tcggcaggta gccaccggcg tcgggcagcg aggtgtagaa cagccgttcg gacacgcgcc
     1201 agccggtgtg gtgcccgtac ttggcgacga gggcctggga cagctcccac gccgtctgcc
     1261 cgtagatctc cccttcgcag tgcgcctcgt agccgagcgg gccgagatag ccggtctgcg
     1321 tgcactgcgc gccgaggttg gtcgtcgtca gcaggccgcg cggcccggcc ggatccaggt
     1381 tgcggaccgg ttcgccggtg aagcggaagc cctcgccgat caccggactg tggctcgagt
     1441 gcaaggcgac gacgtccgcg gtgccctcgc ccgtcgccag gtcgccgagc agcgtgaact
     1501 cgtcgatgcc gtgcccccac tcgtgcgtga tcacgtcggc aatctcgccg gtgttgttgc
     1561 agattccgct cgcccggaag aaattgacgt tgccgtcgta gaacgcgttg caggcgcgat
     1621 cgatgttggc gaccgacgtg acgctcgtga agagccacgg caggccgggt agccacttca
     1681 acgcgatgcg acgcacctgg ttcaggtggt agaaggtgtt gcgctcggca cgggtcgaga
     1741 agccgttgcc gaactggtcc acgcccccga tgccgaagtc gacgtagccg gcgccgaggt
     1801 cgaactggaa gtgggcctgg gtcggatcga ggcagccctg gcaatcggtc gcgaaccacg
     1861 ggccgtccag tccggagacg gccgcgccgc caccgtagtc gaaccgaccg gcgacgtccg
     1921 agctcaccgg attgccgtcg atcgtcagcc cgaccgccgg cagcgggacc gaaacctcgt
     1981 tctgttcgtg gaccgatcgg ggaaagaccc cgccgcgcac gcggccgtaa cggttga
//
LOCUS       DASPGN010000065         3583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1534224, whole genome shotgun sequence.
ACCESSION   DASPGN010000065 DASPGN010000000
VERSION     DASPGN010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534224"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VD788_01890"
     CDS             <1..406
                     /locus_tag="VD788_01890"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HXV75041.1"
                     /translation="IVVAIIGIIAAIAIPNLLNAIDRGKQKRTMADLRSIGTAIESYA
                     IDNVSYPTAGTNLASLTAVLEPLYLRQAPTNDGWNRTFSVACAATEYTVCSGGKDGGA
                     CVNDAGGTTTSFNDSITFANGQFVQWPEGAQQ"
     gene            485..3559
                     /locus_tag="VD788_01895"
     CDS             485..3559
                     /locus_tag="VD788_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV75042.1"
                     /translation="MRGTSLGRPGSLRRLGSSLACAVVFGAPSNAEPPEPPPFYAIRD
                     VRVVSGAGETIERADVLLADGLIEAVGRDLEIPADAWVVDGDGLMLFPGLIDAMTDFG
                     LPARADDDDEGGREPGDAAEIRGPEDRPRTTPWLAAADILKEDARTKKWREAGFTGAV
                     TAPGEGLVAGQAAWINLGDESDAEQAVVATPVAMRLDFSTPRRSRSYPSSLMGVLSYI
                     EQLLSDTAHYGAAHEAYRANPLGVERPPYDRALESLDAAIEAGIPFLMPGQLGREIDR
                     AVRLAGDHGLPLVIYGGQGAYARVDALRDAGVPVLVSLNWPEAEKDRDPEADTPFRTH
                     YHRRFAPTTPAALVHAGVRIAFYSDGLALPSEVFDRVRVAIDAGLGQHEALAALTSGP
                     AGIYRVADRLGTIERGKIANLVLATDWPWAEGAEIKAVFVDGRKYEKHESDEPSEPPS
                     ADVSGTWAMTLTTPRGSREMTARLKMAADGKVTGELSGERGTTPVEQGRMSAELLRFK
                     TTRTMGGRSITSLWSLTVEGEGLAGSATAGPMSMDVVGRRTAAGEPADDDRAAGEPAV
                     SLEEFAVALALYQGPVARNDRFAITNATIWTVSGETIEHGTVVVEGGKIVAVGREASV
                     GDGTRVIDGAGGHLIPGIVDAHSHIAIEGGVNEGSLAVTAMVAVGDVVNPDDVAIYRA
                     LAGGVTTANLLHGSANPIGGQNQVIKLRWGADAAGLAFDGAPPGIKFALGENPKRSNF
                     RGARVAERYPQTRMGVMDVIRQAFNDAVQYRLEWEEYQRGERPLAPRRDLELEALVEI
                     LDGQRLVHSHCYRADEILQLIRLAEEYGFRIATLQHVLEGYKVADEIAEHGAGASTFS
                     DWWGYKVEAYDAIPYNAALMSERGVLVSVNSDSGEEMRHLNQEAAKAVKWGGMDEIDA
                     LALVTRNPAIQLGIGDRVGSIEVGKDADLVLYDGHPLSMRSVVQKTFVDGDLYFDREH
                     DRQRQAAIDAIEAELAPAAKDDEGKAPPDEAVSKPSPAVAWQDGGYSCREER"
BASE COUNT          581 a         1183 c         1304 g          515 t
ORIGIN      
        1 gatcgtcgtc gcgatcatcg ggatcatcgc ggcgatcgcg atcccgaact tgctgaacgc
       61 gatcgaccgc ggcaagcaga aaagaacgat ggccgacctg cgctcgatcg gcaccgcgat
      121 cgagtcgtac gccatcgaca acgtgagcta ccccaccgcc ggcaccaatc tcgcaagcct
      181 gaccgcggtg ctcgagccgt tgtatctgcg ccaagctccg acgaacgacg gctggaaccg
      241 gacgttcagc gtggcctgcg ccgcgaccga gtacaccgtc tgttccggcg gaaaggacgg
      301 cggggcgtgc gtcaacgacg cgggcggcac gacgacgagc ttcaacgact cgatcacctt
      361 cgccaacggg cagttcgtcc agtggccgga gggcgcgcag caataatccg acgctggcct
      421 tcgggccgcc cggtcgttta caatccggcg ttccacgttc gtcgcactct cgaggagtcg
      481 cgtaatgcgc ggtacgtcgc tcggcaggcc gggttccctt cggcggctcg gttcgtcgct
      541 ggcgtgcgcc gtcgtcttcg gcgctccgtc gaatgccgag ccgccggagc caccgccgtt
      601 ctacgcgatc cgcgacgtgc gcgtggtgtc cggagcgggc gagacgatcg agcgggccga
      661 cgtgctgctg gccgacggct tgatcgaagc ggtcgggcgg gacctcgaga ttccggccga
      721 cgcatgggtc gtcgacggtg acgggctgat gctctttccg gggctgatcg acgcgatgac
      781 cgatttcgga ctgcccgcgc gagccgacga cgacgacgag ggcgggcgcg agcccggtga
      841 cgccgccgag attcgcggcc cggaggaccg cccgcggacg acgccgtggc tcgcggccgc
      901 ggatatcctg aaagaagacg ctcggacgaa gaaatggcgc gaggccggct tcaccggcgc
      961 cgtgacggcg ccgggcgagg gtctcgtcgc cggccaggcc gcctggatca acctcggcga
     1021 cgagtccgat gccgagcagg cggtcgtcgc gacgcccgtc gcgatgcgac tcgacttctc
     1081 cacgccgcgg cgctcgcgca gctacccgtc gtcgctgatg ggcgtgctgt cctacatcga
     1141 gcaactgctg tcggacacgg cgcactacgg tgcggcccac gaggcctatc gggccaaccc
     1201 gttgggcgtc gaacggccac cctacgaccg cgcgctcgag tcgctcgacg cggcgatcga
     1261 ggccggcatc ccctttctga tgccgggtca gctcgggcgc gagatcgacc gtgcggtccg
     1321 cctggccggc gaccacgggc ttccgctcgt gatctacggc ggacagggcg cctacgcccg
     1381 cgtcgacgcg ttgcgcgacg ccggcgtgcc ggtgctggtc tcgttgaact ggcccgaggc
     1441 cgagaaagac cgcgacccgg aggcggatac gccttttcgc acccactacc accggcgctt
     1501 cgcgccgacg acgcccgcgg cgctggtgca cgccggcgtg cggatcgcgt tctattccga
     1561 cggcctggcc ctgccgagcg aggtcttcga ccgggtgcgc gtcgcgatcg acgccggcct
     1621 cggccagcac gaagctctcg ccgcgctgac gagcgggccg gccgggatct accgagtcgc
     1681 cgatcgtctc ggcacgatcg aacggggcaa gatcgcgaac ctcgtgctcg ccaccgattg
     1741 gccgtgggcc gagggcgcgg agatcaaggc ggtgttcgtc gacggtcgca agtacgaaaa
     1801 gcacgagagc gacgagccga gcgagccgcc gtcggccgac gtcagcggaa cctgggcgat
     1861 gacgctgacc acgccgcgcg gcagccgcga aatgacggcg cggttgaaga tggcggccga
     1921 cggcaaggtc acgggcgagc tgtcgggcga gcgaggtacg acaccggtcg aacaagggcg
     1981 gatgagcgcc gagctgctgc ggttcaagac gacccgtacg atgggcggtc gctcgatcac
     2041 ctccttgtgg tcgttgacgg tcgagggcga gggtctggcg ggatcggcca cggccgggcc
     2101 gatgtcgatg gacgtcgtcg gccggcgcac cgcggcgggc gagccggccg acgacgaccg
     2161 ggcggccggt gagcccgcgg tctcgctcga ggaattcgcc gtcgcgctcg cgctctatca
     2221 gggcccggtc gcacgcaacg accgcttcgc cattaccaac gccacgatct ggacggtgtc
     2281 gggcgagacg atcgagcacg gcacggtcgt ggtcgagggc ggcaagatcg tcgccgtcgg
     2341 ccgcgaggcg tcggtcgggg acgggactcg cgtgatcgac ggcgcgggcg gccacctgat
     2401 acccggcatt gtcgatgcgc actcgcatat cgcgatcgaa ggcggggtga acgagggctc
     2461 gctggcggtc accgcgatgg tcgccgtcgg cgacgtggtc aatccggacg atgtcgcaat
     2521 ctaccgcgcg ttggccggcg gggtcacgac ggcgaacctg ctccacgggt cggccaaccc
     2581 gatcgggggc cagaaccagg tgatcaagct gcgctggggc gcggacgccg cgggcctcgc
     2641 gttcgacggc gcaccgcccg gaatcaagtt cgcgctcggc gagaatccca agcggtcgaa
     2701 cttccgcgga gcgcgtgtcg cggagcgcta cccgcagacg cggatggggg tgatggacgt
     2761 catccgccag gctttcaacg atgccgtgca gtaccgactg gagtgggagg agtaccaacg
     2821 gggggagcgc ccgctcgcgc ctcgacgaga cctcgagctc gaggcgctgg tcgagatcct
     2881 cgacggccag agattggttc attcgcactg ctaccgcgcg gacgagatcc tgcagttgat
     2941 ccggctcgcc gaggagtacg gctttcgcat cgccacgctc cagcacgtgc tcgagggcta
     3001 caaggtggcc gacgagatcg ccgagcacgg cgccggcgct tcgactttct ccgattggtg
     3061 gggttacaag gtcgaggcct acgacgccat cccctacaac gcggcactga tgtccgagcg
     3121 cggcgtgctg gtttcggtca attccgacag cggcgaggag atgcgccacc tgaaccagga
     3181 ggcggcgaag gcggtgaaat ggggcgggat ggacgagatc gacgcgctgg cattggtcac
     3241 gcgcaacccg gcgatccagc tcggcatcgg cgatcgggtc ggttcgatcg aagtcggcaa
     3301 ggatgcggat ctggtgctgt acgacggcca cccgctgtcg atgcgctcgg tcgtgcagaa
     3361 gaccttcgtc gacggcgatc tctacttcga ccgagagcac gaccggcaac gccaggccgc
     3421 gatcgacgcg atcgaagccg agctggcgcc ggccgcgaag gacgacgagg ggaaagcgcc
     3481 gccggacgag gcggtgtcga agccgtcgcc cgccgtcgcc tggcaggacg gcggctacag
     3541 ctgccgggag gaacgatgat gcgcagcgga ggttcggcga tgc
//
LOCUS       DASPGN010000066        16918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_363378, whole genome shotgun sequence.
ACCESSION   DASPGN010000066 DASPGN010000000
VERSION     DASPGN010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 16918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16918
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363378"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VD788_01900"
     CDS             <1..891
                     /locus_tag="VD788_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75043.1"
                     /translation="SLRDYGARLEFEPGSLERIEARRAALARLMLRYGADERAVLDAR
                     AAAARELEAIAAHDDRVARAAERLREAERDYAACAVELGQRRRTAAGALGAAVEKQLT
                     ALALGRARFSVGVTAANGEAVEVAGFGALVFHPRGAERAEFRLAANPGEPERPLHKVA
                     SGGELSRVMLGLHAAVDGAGEDRVLIFDEIDSGVGGRVADAVGARLASLARRGQVLCV
                     THLPQVAVYADAHFSVRKWVEGDRTHTAVERLAPERRIEELARMLGGKRATDASRRHA
                     AELLDGAGRGGAGRAPRGRA"
     gene            888..1391
                     /gene="coaD"
                     /locus_tag="VD788_01905"
     CDS             888..1391
                     /gene="coaD"
                     /locus_tag="VD788_01905"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXV75044.1"
                     /translation="MKKRIAVYPGTFDPVTNGHLDLADRGRRHFDRLVMAILHNDDKQ
                     PLFTVQERIGFLRDAVRGWGNVDVDSFDGLLVDYARRIGASVIVRGIRALTDFEYEMQ
                     MAMMNRRLAPDLEAVFLLPSEEYSFVSSRLVREVASLGGAIERLVPANVARALAERYP
                     QDRRGPD"
     gene            1424..2782
                     /locus_tag="VD788_01910"
     CDS             1424..2782
                     /locus_tag="VD788_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HXV75045.1"
                     /translation="MRRFTLLVVLAAAAVIDLPAADTPVAGETGPIVRLIEFDGPITP
                     ISALRITQAIDEAERAGDAFVLIELDTPGGLVDSMETIVKRMLAAELPIVVWVGPSGA
                     KAASAGFFLLLSADVAAMAPGTRTGAASGVHGFGSSEDGDVMLRKVNEDLAALIRSIA
                     DRRGRDVGAAERAVFDAKSYEEQVALELGLVDLIAPSRDGLLQLLDGMEITRFDGSTS
                     TIRTAGARFEVTEFSLRHEFMELLSVPAVAAFLFFAGLLGIYVEFTHPGVVFPGVVGA
                     LCLLLFALSARVLPISTIGVLLILLGVLMFVLEIKVTSFGMLTVGGAVALAIGGWMLV
                     EGPIPELRVPPTFLVPLVVAVTAVCAFVLRLAVAAQRLRVTSGREGLSGEIGTVSVDL
                     APDGKVFVHGELWNAVADGGSLPRGTRVRVVRVDEMMLTVTPVETTGPDGVPPIGESV
                     RT"
     gene            2791..3540
                     /locus_tag="VD788_01915"
     CDS             2791..3540
                     /locus_tag="VD788_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HXV75046.1"
                     /translation="MNPFTLGIVAVFVFLGMLVTYAIRILPEYERGVVFRLGRLRPSD
                     YGPGIFLLIPVVDKMVRVSLRTVVHDVPPQDVITRDNVSVKVNAVVYFRVMNPRKAIV
                     EVENYHYATSQLSQTTLRSVLGQVELDELLSEREKINHELQAILDKQTDPWGIKVSAV
                     EVKHVDLPSEMQRAMAKQAEAEREKRAKIIHAQGESQAASTLAQAAAELSKNPSSMQL
                     RYLQTLTEIASERNSTLIFPLPVELLRSFIK"
     gene            3568..4644
                     /locus_tag="VD788_01920"
     CDS             3568..4644
                     /locus_tag="VD788_01920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXV75047.1"
                     /translation="MSSVGKKTVEDPALAGLAGILDPPFVEALVAAFRSLEVSGHAKA
                     MLDTADSWCRPRFGVAYALGEATRSLVPVTSTAGDEEDPRFLAFSPIAVSRLAQLVGE
                     TATSWEGFGPARGLVGEWSGDEPRATLTLPLVSGKGRLAAAIVLLLDSAPDGETIERL
                     ATLLRLTRPALDNALRMLAMRDLVIKDDTAQCFNRRHFEESLPEELARATRYRSPLSL
                     IFLDMDNLKEVNTRFGHAMGSQTLHEVSLRVRSKTRKFDKLFRFGGDEFCILLPETDW
                     QGALEVAERVREAIVSRPFLESHVNDGQVVAMSASLGIAAFPLHARSRVELIQRADRA
                     MQRVKKSTKNAVGIAQIEEAENVE"
     gene            4634..5212
                     /locus_tag="VD788_01925"
     CDS             4634..5212
                     /locus_tag="VD788_01925"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXV75048.1"
                     /translation="MSNDGGNGEEVREFRLEGLTLVVVATALVLALGGAFMLGRWVER
                     GSAPPSSGGTAAAGEDPLANLARADEPADVDETSDIFDRADETATEPEREVRSDSGVT
                     RGDAPASAPTESSGNYFVQVWAGRDRDAAELLVDKLERDGYAVTIVSDRVEGDTLFKV
                     RVGGYPTEAEARRTSGELEGKGYRGAWVTSAS"
     gene            5355..6425
                     /gene="prfB"
                     /locus_tag="VD788_01930"
     CDS             5355..6425
                     /gene="prfB"
                     /locus_tag="VD788_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HXV75049.1"
                     /translation="MRCGGSFDVAGAGASLAELDGRLAQPEVWSDPDRAKTLQQQRSR
                     LLERIESAERLGTLLEEAQTMLELAREGESVGDELIAAVDSLESVVEGVELETMLAGE
                     HDAADAIVEIHPGAGGTESQDWAAMLLRMYTRWAESRGFDVRLLDYQDGDEAGIKSAT
                     LEVSGPNAYGYLRVERGVHRLVRISPFDAQGRRHTSFASVDVIPETDEAIQVEIEDKD
                     VRVDTYRASGAGGQHVNKTDSAVRLTHAPTGIVVTCQNERSQHRNKEVAYNILRAKLY
                     DLAQREAEARLAAEVGEKKKIEWGSQIRSYVLAPYRLVKDHRTNHEVGNADRVLDGDL
                     DDFIRAALMAQREGERPRAATD"
     gene            6442..7512
                     /gene="hrcA"
                     /locus_tag="VD788_01935"
     CDS             6442..7512
                     /gene="hrcA"
                     /locus_tag="VD788_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HXV75050.1"
                     /translation="MGVRGIADWSPIELGRHQADVLREIVRQYILTGEPVGSASVGRS
                     AKLALSPASIRNVMGELESLGLLSQPHTSAGRVPTDRAYRIYVDRLIHRTRIKPEQAE
                     AIVRALTATGGEIEGLLGEASRQLSLLSNQVGLVLAPELDRLIVDQIEFVRLDSGRVM
                     AVLVTRSGVIHNRIIHVAEPIDPPELERIGRYITGEFGGRTLPAMRELLEQRLGQDRL
                     AYDRAMARALELGREIAGSDDIESALFVDGASNLLHKRDLADIGVLQSLFRTLEDKKT
                     LIDLLGRLIESRGVRVVIGEENPLADLNRCSLVASNYHAGDRVIGTLGIVGPVRMPYG
                     RAMALVEHLSGVLTRVFSEPRN"
     gene            7617..8306
                     /gene="grpE"
                     /locus_tag="VD788_01940"
     CDS             7617..8306
                     /gene="grpE"
                     /locus_tag="VD788_01940"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXV75051.1"
                     /translation="MSDSERDLADRWDEHDDDEIEIIEIVGLDDDSPAASRVDRLALD
                     GGDDDELVIELDDDEERADPIAAPSDVPDPTVVDDNERFARLSADFENLRKRMDRERV
                     AHERHAGAQLVARLLPVLDNFERALSNAREAQTDPRFSEGVAMIFRQLLDELRKEGLV
                     AVDTVGEPFDPAVHEAVATTEHEQLPPNTVVEELQRGYLLHDRLLRPALVKVTVDPAN
                     DRAPGEPPKDR"
     gene            8310..10166
                     /gene="dnaK"
                     /locus_tag="VD788_01945"
     CDS             8310..10166
                     /gene="dnaK"
                     /locus_tag="VD788_01945"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXV75052.1"
                     /translation="MGKAIGIDLGTTNCCMAVMEGGKPRVIATREGARTMPSIVAFSS
                     RGERLVGQIAKRQALTNPHNTIYAVKRLVGRKFESPEVAKAMQLVPYEIVPSKNRDAW
                     IRVRDRDFSPPEMAGFLLAFLKETAEDYLGTEVSEAVITVPAYFDDAQRQATKDAGRI
                     AGLHVLRIVNEPTAASLAYGLDQGTQSRRIAVYDLGGGTFDISILQLGEGVFEVKSTS
                     GDTFLGGEDFDQRIVDWLVTMFREESRIDLRTDRMALQRLKEAAEKAKCELSHEITAE
                     INLPFISADETGARHLHTTLSRQKLEELVDDLVDKTRGPCEEALKLAGMKPADLDEVL
                     LVGGQTRMPKVVETVRDIFGREPSRDINPDEVVGIGAAVQAGVLKGDVKDLVLLDVTP
                     LSLGIETRGGMFTTIIERNATIPTRKSKIFTTVADNQTKVQIHVLQGEREIAAHNKSL
                     GQFELVGLPPAPRGATQIEVSFDIDSNGIVSVQARDLATQREQKILVTPSSGLTEEEI
                     GVIIDDAKKHSEEDRKRAEFIRARARLEGLVESNEKTYNEFGSMLAPDQQAAARKILD
                     RAKKALESGSAAECTEALEKIAEMGRILSEVILYDPGTFSSVDKSEVDSGEA"
     gene            10170..11276
                     /gene="dnaJ"
                     /locus_tag="VD788_01950"
     CDS             10170..11276
                     /gene="dnaJ"
                     /locus_tag="VD788_01950"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXV75053.1"
                     /translation="MSERDYYEILGVERDADLAAIKKAYRRAALRFHPDKNPGDRQAE
                     ESFKDAAEAYAVLSNEDKRQLYDRFGRSGLSGNTGFGGFDADVFGDFSDILGDLFGFG
                     SVFGGGRRRARAGTGRDLRYDLEIEFKEAVIGLQTQIVVPRLERCDTCEGRGAQPAGI
                     ESCGPCRGRGQVAFQQGFFTIARPCAHCGGSGRRITDPCGECRGQGRVQRERTLTVRI
                     PAGVDHGTRIRLVGEGEGGVGGGPPGDLYVVLGVADDPFFERDGLDLHCEIEIGMAQA
                     ALGSRVEVRRIEGAETVEIPAGTQSGSQIRLRGQGVPALDGRGRGDQVVTVRVRTPKR
                     LSAAQRELFEKLAELERTESEDPGLFDRVKKIFN"
     gene            11289..12203
                     /locus_tag="VD788_01955"
     CDS             11289..12203
                     /locus_tag="VD788_01955"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HXV75054.1"
                     /translation="MTAERSSGDGERSGAAGRPRVWSAVVIELPAALVDEALAIVASD
                     SSGAELGEDGVLRRLAVWFERDEAAEGALARVRRWLSRRGLTADARLERVEDGHWAER
                     YQAGLVPFDLGARFIVDPSGTELDEPRAGVRTYIHLAPGGAFGTGEHPTTRLCVGLLE
                     SLVAPGTRWLDLGCGTAILSVVARYLGASRVAAVDLDGEACQVARQVLARNGLSRQVL
                     LWNGSLAELAPDCWDGVVANVELPFFLENSARLGRLPAVGGLLIASGFLGDDARLVAL
                     RLGRAGLSPIRIEIDPPWAALAARRVAR"
     gene            12200..12949
                     /locus_tag="VD788_01960"
     CDS             12200..12949
                     /locus_tag="VD788_01960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HXV75055.1"
                     /translation="MSSRRWRARHRPLPDTAGATIRLDRDESHHVHRVLRLGRGASLG
                     VFDGLGLEWDGVIDGIDDGIVVVRLVRRLDEVVEPPVELVLFQTVCRNERMEWVIQKT
                     TELGIAAIRPISAERAEARPPTAARVARWRRIALEAAKQCGRRTVPGIEPADALAAPA
                     AGVLPLLFDTGRDCRPLAERLVPLPDGPVWLAVGPESGFSADEHCRHVGEGWLPTGLG
                     PRTLRAETAGIVAAAIVLHLCGDLGRASDAG"
     gene            13011..14195
                     /locus_tag="VD788_01965"
     CDS             13011..14195
                     /locus_tag="VD788_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXV75056.1"
                     /translation="MHINDLLKSATDQGSSDLHLKVGSYPVIRVDGRLRPMTDQKRLM
                     QEDTIAMAFSIMSARQKQKFKDHFEIDMAYSVPGLGRFRVNVFQQRGTVGLVLRVIPI
                     KILSIRELHLPTVLERIAHERRGLILVTGTTGSGKSTSLAAMIDFVNSTRTEHIMTIE
                     DPIEFLHRDKKSLVNQREVEVDTKSFAFALRSALRQDPDVILVGEMRDYETIETALNA
                     AETGHLVLSTLHTVDATETINRVISVFPPHQQKQVRLQLGAVLRAIISMRLVPRKDGN
                     GRVPAVEVLRSTAYIRDCVENKEKTKLIASAIAAGTSQYGMQTFDQSLYNLLRADLIS
                     FEEALRQATNPDEFKLKVAGIQSASDMATEAMEAMLQQSQAGGGTHNPRDPDSPFEFS
                     NS"
     gene            14208..14696
                     /locus_tag="VD788_01970"
     CDS             14208..14696
                     /locus_tag="VD788_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HXV75057.1"
                     /translation="MIDGDDRYDRVEAHALRLLAGRDRTAVELRRHLTAADHPPDEVE
                     RVITRLRQAGYLDDGRLALAAILADVAAGRAAARIDRRLLERGVEPALIERAWDAAAA
                     DHAVDRARLLAAALERRLVHVARPLDRRAIRRVYNAMLRAGFDADEVAAALEPHLPGD
                     AG"
     gene            14730..>16918
                     /gene="alaS"
                     /locus_tag="VD788_01975"
     CDS             14730..>16918
                     /gene="alaS"
                     /locus_tag="VD788_01975"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXV75058.1"
                     /translation="MTANETRKRFLSFFADRGHRVVPSSPLVLPNDPTLLFANAGMNQ
                     FKDVFTGREVRDYLRAASSQKCLRVSGKHNDLETVGRTPRHHTFFEMLGNFSFGDYFK
                     KEAVEYAWELVTGVFGLPEERLWASVFGGTATLPADEEAFRLWTETIGLPAERVLRLG
                     EKDNFWRMGETGPCGPCSELHFDLGADLASVEPGSNPATDDRRFLEIWNLVFMQFDQQ
                     SDGALVPLPAPSIDTGMGLERIASVLQGKRSNYDTDLFRPILQAVAERAAVRYGADEE
                     TDLALRVIADHGRAFCFLTADGVVPANDNRGYVLRRLLRRAIRFGRKLGIDEPFLHHV
                     TPAVVDGLGDHYPELLAARDAMLEIGKLEEQRFADTLSTGLQVLEQALAGTEPDRSTG
                     RPVLAGADLFRLYDTFGFPLDLARDVADERGIELDVAGFEREMAKQRARAQASWKRVK
                     NERASGVYSELVDRVQSRFAGYSVERLDGVPVAAVVRDGRPVDVLEGGEEGEVVLELT
                     PFYAEAGGQVADTGFLAAPSGHAEVIDVQRPLPGLIVHRVRLVSGALENRERVTAEID
                     TRRREATRRNHTATHLLHAALREVVGTHVKQAGSLVAPDRLRFDFSHFAGLADHALAD
                     VELLINRKVMEDLAVETDEMDLDDAIAAGAMALFGEKYGERVRVVRIGDFSLELCGGT
                     HTARTGEIGLVKLLHERGVASGTRRVEAVSGEGALSAFGDDQRVVRAL"
BASE COUNT         2667 a         5636 c         6050 g         2565 t
ORIGIN      
        1 tcgctgcgcg actacggtgc acggctcgag ttcgagccgg ggagcctcga gcggatcgag
       61 gcgcgtcggg cggcgctcgc ccggctgatg ctccgctatg gcgcggacga acgggcggtg
      121 ctcgacgcgc gcgccgcggc ggcccgcgag ctcgaagcga tcgcggcgca cgacgaccgc
      181 gtggcgcggg ctgcagaacg actgcgcgag gccgagcgcg actacgccgc ctgcgccgtc
      241 gagctcggcc agcgccgccg gaccgccgcc ggcgcgctcg gcgccgccgt cgaaaaacag
      301 ctcaccgcac tcgcactggg cagggcccgc ttctcggtcg gcgtcaccgc cgcgaacggc
      361 gaggcggtcg aggtcgccgg cttcggtgcg ctcgtcttcc atccgcgcgg cgccgagcgc
      421 gcggagtttc gcctggcggc caacccggga gagccggaac gaccgctgca caaggtcgcg
      481 tcgggcggcg agctgtcccg cgttatgctg ggcctgcacg ccgccgtcga cggggcgggg
      541 gaggaccgtg tgctgatctt cgacgagatc gacagtggcg tcggcggacg ggtggccgac
      601 gcggtcggcg cgcgactggc gtcgctcgcg cgccgcggtc aggtgctgtg tgtcacccac
      661 ctgccgcagg tggcggtcta cgccgacgcg cacttttcgg tccgcaagtg ggtcgaaggc
      721 gatcggacgc ataccgccgt cgagcgattg gcgcccgagc gacggatcga agagctggcg
      781 cgtatgctcg gcggcaagcg ggcgaccgac gcctcgcggc gccacgccgc cgagctgctc
      841 gacggcgcgg gccgcggcgg tgccggacga gcgccgcggg ggcgggcgtg aaaaagcgga
      901 tcgcggtcta ccccggcacc ttcgatccgg tgacgaacgg ccacctcgac ctcgccgacc
      961 gcggccggcg tcatttcgac cggttggtca tggcgatcct gcacaacgac gacaaacagc
     1021 cgttgttcac cgtccaggag cggatcgggt tcctgcgcga tgccgttcgc ggctggggca
     1081 acgtcgacgt cgacagcttc gacgggctgc tcgtcgacta cgcgagacgg atcggcgcgt
     1141 cggtgatcgt gcgcggcatc cgcgcactga ccgacttcga gtacgagatg cagatggcga
     1201 tgatgaaccg gcgtctcgcg cccgatctcg aggcggtatt cctgctgccg tccgaggagt
     1261 actcgttcgt gtcgagccgg ctggtgcggg aggtggcttc gctcggcggc gcgatcgagc
     1321 gtttggtgcc ggcgaacgtc gcccgggcgc tcgccgagcg ttatccgcag gatcggcggg
     1381 gaccggattg agctttgccg gcgggcctcc tatactcctc gtcatgaggc ggttcacgct
     1441 gctcgtcgtg cttgccgccg ccgccgtcat cgacctcccg gcggccgaca ccccggtggc
     1501 gggcgagacc ggcccgatcg tccggctgat cgagttcgac ggcccgatca ccccgatcag
     1561 cgcgctgcgc atcacccagg cgatcgacga ggcggagcgg gccggcgacg cgttcgtctt
     1621 gatcgagctc gacacgccgg gtggcctcgt cgattcgatg gagacgatcg tcaagcgcat
     1681 gctcgcggcc gagctcccga tcgtcgtctg ggtcgggccg tcgggcgcca aggccgcgtc
     1741 cgccggtttc ttcctgctgc tgtcggccga cgtggccgcg atggcacccg gcacgcgcac
     1801 cggcgcggcg agcggggtcc acggcttcgg cagcagcgag gacggcgacg tcatgctgcg
     1861 caaggtcaac gaggacctgg cggcactgat ccgttcgatc gcggatcggc gcggacgcga
     1921 cgtcggggcg gccgagcggg cggtgttcga cgccaagtcc tacgaggaac aggtcgcgct
     1981 cgagctcggc ctggtcgacc tgatcgcgcc gtcgcgcgac gggctgctgc agttgctcga
     2041 cgggatggag atcacgcggt tcgacggctc gacgagcacg atccgcaccg cgggagcgcg
     2101 cttcgaggtg accgagttct cgctgcggca cgagttcatg gagctgctgt cggtccccgc
     2161 cgtcgccgcg ttcctgttct tcgccggcct gctcgggatc tacgtcgagt tcacccaccc
     2221 cggcgtcgtc ttccccggcg tcgtcggcgc gctgtgcctg ctgctgttcg cgttgtcggc
     2281 gcgtgtgctg ccgatctcga cgatcggggt cttgctgatc ttgctcggcg tgctgatgtt
     2341 cgtgctcgag atcaaggtga cgtcgttcgg catgttgacc gtcggggggg cggtcgcgct
     2401 cgcgatcggt ggctggatgc tcgtcgaggg tccgatcccc gagcttcgcg tgccgccgac
     2461 cttcctcgtc ccgctcgtcg tcgccgtcac cgcggtctgc gccttcgtgc tccggctcgc
     2521 agtggcggcg caacggctgc gcgtgacctc ggggcgggaa gggctgtcgg gcgagatcgg
     2581 cacggtcagc gtggatctcg cgcccgacgg caaggtcttc gtgcacggtg agctgtggaa
     2641 cgccgtcgcc gacggcggga gcctaccgcg cggaacgcgc gtccgcgtcg tgcgcgtcga
     2701 cgagatgatg ctgaccgtga caccggtcga gacgaccggc ccggacggag tcccgccgat
     2761 cggtgagtcg gttcgaacct aggaggagag atgaatccgt tcacgctcgg gatcgtcgca
     2821 gtcttcgtgt tcttggggat gctcgtaacc tacgcgatcc gcatcctccc cgagtacgag
     2881 cgcggggtgg tgttccgcct cggccggctg aggccgagcg actacggacc ggggatcttc
     2941 ttgctgatcc cggtcgtcga caagatggtg cgggtttcgc tgcggaccgt cgtccacgac
     3001 gtaccgccgc aggacgtcat cacgcgggac aacgtttcgg tcaaggtcaa cgcggtcgtc
     3061 tatttccgcg tgatgaaccc ccgcaaggcg atcgtcgagg tcgagaacta ccactacgcc
     3121 accagccagt tgtcgcagac cacgctgcgc tcggtcctgg ggcaggtgga gctggacgaa
     3181 ctgctgtccg agcgcgagaa gatcaaccac gagctgcagg cgatcctgga caagcaaacc
     3241 gacccgtggg gcatcaaggt ctcggcggtc gaggtgaaac acgtcgattt gccctcggaa
     3301 atgcagcgcg cgatggccaa acaggccgag gccgagcgcg aaaagcgggc caagatcatc
     3361 cacgcccagg gcgagtccca ggccgcctcg acgctggccc aggcggctgc cgaactcagc
     3421 aaaaacccct cctcgatgca gcttcggtac ctccagacct tgacggagat cgcctcagag
     3481 cgaaattcaa ctctgatctt cccgctcccg gtggagctgc tgcggtcgtt catcaagtag
     3541 acgggggacc gtgagggaca ggcgcgcgtg agctcggtcg gcaagaaaac ggtcgaggac
     3601 ccggccctgg ccggcctggc cggcatcctc gatccgccgt tcgtcgaggc cctggtggcg
     3661 gccttccgct cgctcgaggt ctccgggcac gccaaggcga tgctcgacac cgcggacagc
     3721 tggtgccggc cgcgcttcgg cgtggcctac gcgctcggcg aagcgacgcg ctcgctcgtc
     3781 ccggtgacct cgacggcagg tgacgaggag gacccgcggt tcctcgcctt ctcgccgatt
     3841 gccgtctcac gtctggccca gctcgtcggc gagacggcca ccagctggga gggcttcggg
     3901 ccggcccgtg ggctggtcgg cgaatggtcc ggcgacgaac cgcgcgcgac gctgaccttg
     3961 ccgctggtca gcggcaaggg aaggctggcc gccgcgatcg tgctgttgct cgattccgcg
     4021 ccggacggcg agacgatcga gcgcttggcg acactgctgc gcctgacgcg cccggcgctc
     4081 gacaacgcgc tccgcatgct cgcgatgcgc gatctggtca tcaaggacga caccgcccag
     4141 tgcttcaacc gacgccactt cgaagagagc ctgccggaag agctggcccg cgccacccgc
     4201 taccgctcgc cgttgtcgct gatcttcctc gacatggaca atctcaagga ggtcaacacg
     4261 cggttcggtc atgcgatggg cagccaaacg ctccacgagg tctcgctgcg cgtgcggtcg
     4321 aagacccgca agttcgacaa gttgttccgc ttcggcggcg acgagttctg tatcctgctg
     4381 ccggagaccg attggcaagg tgcactcgag gtcgccgagc gcgtccgcga ggcgatcgtc
     4441 agccggccgt ttctcgagag ccacgtcaac gacggtcagg tcgtcgcgat gtcggcgtcg
     4501 ctcgggatcg ctgcattccc gcttcacgcc cgctcgcggg tcgagctgat ccagcgtgcc
     4561 gaccgagcga tgcagcgcgt caagaagtcg acgaagaacg ctgtcgggat cgcacagatc
     4621 gaggaggcag agaatgtcga atgacggggg gaacggcgag gaggtccgcg agttccggct
     4681 cgaggggctg acactggtcg tggtcgccac cgcactggtg ctcgcgctcg gtggcgcgtt
     4741 catgctgggc cgctgggtcg agcgcggcag cgcgccgccg tcgtccggcg ggactgccgc
     4801 ggccggcgag gacccgctgg ccaacctggc tcgggccgac gagccggccg acgtcgacga
     4861 gacgtccgat atcttcgacc gtgccgacga gaccgccacg gagccggagc gcgaggtccg
     4921 gagcgattcg ggcgtaacgc gcggcgatgc gccggcgtcg gcgccgaccg agtccagcgg
     4981 aaactacttc gtccaggtct gggccggccg cgaccgcgac gcggcggaac tgttggtcga
     5041 caagctcgaa cgggacggct acgccgtgac gatcgtctcc gatcgcgtcg agggtgacac
     5101 cctgttcaaa gtgcgcgtcg gcggctaccc gaccgaggcc gaggcccgcc ggacttcggg
     5161 ggagctcgag ggcaagggtt accgcggcgc ttgggttacg agcgccagct gaccccggcc
     5221 gaaaccctgt gatatgttga ggttcgcccg gtcgatcggt tcgatcgacc accgggcgat
     5281 ggaggattcc gtgttggcca cgaccgatat tcaagggttc tccgagcgca tcgcgcggct
     5341 gggcgagaag gtggatgcgc tgcggaggtt cctttgatgt ggccggcgcg ggcgcgtcgc
     5401 ttgcggagct ggacgggcgt ctggcgcaac ccgaggtgtg gagcgacccg gaccgggcca
     5461 agacgctcca gcagcaacga tcgcggttgc tcgagcggat cgagtcggcc gagcgactgg
     5521 ggacgctgct cgaggaggcc cagaccatgc tcgagctggc ccgggagggg gagagcgtcg
     5581 gggacgagct gatcgccgcc gtcgattcgc tcgagagcgt cgtcgagggc gtcgagctcg
     5641 agacgatgct ggccggcgag cacgacgcgg ccgacgcgat cgtcgagatc cacccggggg
     5701 cgggcggaac cgagtcgcag gactgggccg cgatgctgct ccggatgtac acgcgttggg
     5761 cggagagccg cgggttcgac gtgcggctgc tcgactacca ggacggcgac gaggcaggga
     5821 tcaagagcgc gacgctcgag gtcagcggtc ccaacgccta cggctacctg cgtgtcgaac
     5881 gtggggtcca ccggctggtg cggatctcgc cgttcgacgc ccagggccgg cgccacactt
     5941 cgttcgcgtc ggtcgacgtg atccccgaga ccgacgaggc gatccaagtc gagatcgagg
     6001 acaaggacgt ccgcgtcgac acgtatcgcg cctcgggcgc cggtggccag cacgtgaaca
     6061 agaccgactc ggcggtccgg ttgacacacg cgccgaccgg catcgtcgta acctgccaga
     6121 acgagcggtc ccagcatcgc aacaaggaag ttgcgtacaa tatcctccgc gcgaagctgt
     6181 acgatctggc gcaacgggaa gccgaggcgc gtctcgccgc cgaggtcggc gagaaaaaga
     6241 agatcgaatg gggtagtcag atccgatcgt acgtgctggc gccctaccgg ctggtcaagg
     6301 accaccggac gaaccacgag gtgggcaacg ccgaccgcgt gctcgacggt gatctcgacg
     6361 atttcatccg ggcggcgttg atggcgcagc gcgaggggga gcggccgcgg gccgccaccg
     6421 actagcgggg cgatggtgat catgggagtc aggggcatcg cggactggag cccgatcgag
     6481 ctcggccgac accaggccga cgtcctgcgc gagatcgtgc ggcagtacat cctcaccggc
     6541 gagccggtcg ggtcggcgtc ggtcgggcgc tcggcgaagc tcgccttgtc tcccgcatcg
     6601 atccgcaacg tgatgggcga gctcgagtcg ctcggtctgc tcagccagcc gcacacctcg
     6661 gccggccgcg tgccgaccga ccgcgcctac cggatctacg tcgaccgtct gatccaccgg
     6721 acgcggatca aacccgagca ggccgaggcg atcgttcggg cgctgaccgc caccggcggc
     6781 gagatcgagg ggctgctcgg cgaggcctcg cgccagctgt cgttgctttc gaaccaggtc
     6841 gggctggtgc tggcgcccga gctcgatcgg ctgatcgtcg atcagatcga gttcgtccgt
     6901 ctcgactccg gccgggtgat ggcggtgctc gtcacccgct cgggcgtgat ccacaaccgg
     6961 atcatacacg tcgccgaacc gatcgacccg cccgagctgg agcggatcgg ccgttacatc
     7021 accggagagt tcggcgggcg caccttgccc gccatgcgcg agctgctcga gcaacggctg
     7081 ggacaggacc ggctggccta tgaccgggcg atggcgcgcg ccctcgagct ggggcgggag
     7141 atcgcgggct cggacgacat cgagagcgcg ttgttcgtcg atggcgcgtc gaatctgctg
     7201 cacaagcggg atctggcgga catcggcgtg ctgcagtcgc tgtttcgcac gctcgaggac
     7261 aagaaaacgc tgatcgacct cctcgggcga ctgatcgaat cgaggggggt gcgcgtcgtg
     7321 atcggcgagg agaatccgct ggccgatctc aatcggtgct cgctcgtcgc gtcgaactac
     7381 catgccggcg accgcgtcat cgggacgctg ggcatcgtcg gaccggtccg catgccctac
     7441 ggccgggcga tggcgttggt cgagcacctg tcgggggtac tgacccgggt cttctcggag
     7501 ccgcggaatt gacgccggga cctccgggga caacattcag gacgtggcgg gcgggcacgc
     7561 ccggctgagg gagcagggtc gatccgccgc acccgcggcg acgggcatcg aggacaatgt
     7621 cggattccga gcgggacctg gccgatcgct gggacgagca tgacgacgac gagatcgaga
     7681 tcatcgagat cgtcggactc gacgacgatt cgccggcggc aagccgggtc gatcgcctcg
     7741 cgctcgacgg aggcgacgac gacgagctgg tcatcgagct cgacgacgac gaagagcgcg
     7801 ctgacccgat cgccgcgccg agcgacgtgc cggacccgac cgtggtcgac gacaacgagc
     7861 ggttcgcccg tttgtcggcg gacttcgaga acctgcgcaa gcgcatggac cgcgagcggg
     7921 tggcgcacga acgccacgcg ggggcacagc tcgtggcgcg tctgctgccg gtgctcgaca
     7981 acttcgagcg cgcgctgtcg aacgcccgcg aagcgcagac cgatccgcgc ttctccgagg
     8041 gcgtggcgat gatctttcgt caactgctcg acgagctccg caaggagggt ctggtggcgg
     8101 tcgacacggt cggcgagccg ttcgacccgg cggtgcacga ggccgtcgcg acgaccgagc
     8161 acgaacagct cccgcccaac accgttgtgg aggagctgca acggggttat cttctgcacg
     8221 atcgtttgtt gcgtcccgcg ctggtcaagg tcaccgtcga tcccgccaac gatagggcgc
     8281 ccggcgagcc ccccaaggat cgttgagcca tgggtaaggc gatcggcatc gacctcggaa
     8341 cgaccaactg ctgcatggcg gtgatggaag gcggcaagcc gcgcgtcatc gccacccgcg
     8401 agggtgcccg gacgatgccg tcgatcgtcg ctttctcctc gcgcggcgag cggctcgtcg
     8461 gccagatcgc caagcgccag gcgctgacca acccgcacaa cacgatctac gccgtcaaac
     8521 ggctggtcgg ccgcaagttc gagtcgcccg aggtggccaa ggcgatgcag ctcgtcccgt
     8581 acgagatcgt accgtcgaag aaccgcgacg cgtggatccg ggtccgggac cgtgatttct
     8641 cgccacccga gatggccggc tttctgctgg ccttcctcaa ggaaaccgcc gaggactatc
     8701 tcgggaccga ggtatcggaa gcggtgatca ccgtaccggc ttacttcgac gacgcgcagc
     8761 gacaggccac gaaggacgcc gggcgcatcg ccggcttgca cgtgctgcgg atcgtcaacg
     8821 agccgacggc cgcctcgctg gcctacgggc tcgaccaggg cacgcagtcg cgccggatcg
     8881 ccgtctacga cctcggcggc gggaccttcg acatctcgat cctccaactg ggcgagggcg
     8941 tgttcgaggt caagtcgacc tcgggcgaca ccttcctcgg cggcgaagac ttcgaccagc
     9001 gcatcgtcga ctggctggtg acgatgtttc gcgaagagtc ccgcatcgac ctgcggaccg
     9061 accggatggc gctgcaacgg ttgaaggagg cggccgaaaa ggccaagtgc gagctgtcgc
     9121 acgaaatcac ggccgagatc aacctgccgt tcatcagtgc cgacgaaacg ggggcccgtc
     9181 acctgcacac gactctctcg cgccagaagc tcgaggagct cgtcgacgat ctcgtcgaca
     9241 agacccgcgg tccgtgcgag gaagcgctga agctggccgg gatgaagccg gccgacctcg
     9301 acgaagtgct gctggtcggc gggcagacgc ggatgcccaa ggtcgtcgag acggtccggg
     9361 acatcttcgg ccgcgagccc tcccgcgaca tcaatccgga cgaggtcgtc ggcatcgggg
     9421 ccgcggttca ggcaggcgtg ttgaagggcg acgtcaagga cctggtgctg ctcgacgtca
     9481 cgccgctgtc gctcggcatc gagacgcgcg gcggcatgtt caccacgatc atcgagcgca
     9541 acgcgacgat ccccacgcgc aagtcgaaga tcttcaccac ggtcgccgac aatcaaacca
     9601 aggtgcagat ccacgtgctc cagggcgaac gcgagatcgc cgcgcacaac aagtcgctcg
     9661 gtcagttcga gctggtcggg cttccgccgg caccgcgcgg cgcgacgcag atcgaggtct
     9721 cgttcgacat cgactcgaac ggcatcgtca gcgtccaggc gcgcgatctg gcgacgcagc
     9781 gcgagcagaa gattctggtc acgccgtcga gcggtctgac ggaagaggag atcggcgtga
     9841 tcatcgacga tgccaagaag cactcggagg aggaccgcaa acgcgcggag ttcatccgtg
     9901 cccgagcgcg tctcgagggg ttggtcgaga gcaacgagaa gacctacaac gagttcggct
     9961 cgatgctggc cccggaccag caggccgcgg ctcgcaagat cctggaccgg gcgaagaagg
    10021 ccctggaaag cggaagcgcc gccgagtgta ccgaagccct ggagaagatc gccgagatgg
    10081 gtagaatcct ctccgaggta atcctctatg atcccggtac attctcctcc gtcgacaaat
    10141 cggaggtcga ttcgggcgag gcgtgacgcg tgtctgagcg agactactac gaaatcctcg
    10201 gagtcgagcg tgacgccgac ctggcggcga tcaagaaggc gtaccgccgc gcggcgctcc
    10261 gcttccatcc cgacaagaac ccgggcgacc ggcaagccga ggagagcttc aaggacgcgg
    10321 ccgaggccta cgcggtgctg tccaacgaag acaagcgcca actctacgat cgcttcggtc
    10381 gcagcgggct gagcggcaat accggcttcg gtggtttcga cgcggacgtc ttcggtgatt
    10441 tcagcgacat cctgggcgac ctgttcggct tcggcagcgt cttcggcgga ggtcgcagac
    10501 gcgcacgcgc cgggacgggc cgcgacctgc gctacgacct cgagatcgaa ttcaaagagg
    10561 cggtgatcgg cctccagacc cagatcgtcg tcccgcggct cgagcgctgc gacacgtgcg
    10621 aaggacgcgg ggcgcaaccg gccggcatcg aatcctgcgg cccgtgccgc gggcgcggac
    10681 aggtcgcgtt ccagcagggg ttcttcacca tcgcgcgtcc gtgcgctcac tgtggcggca
    10741 gcggtcgccg tatcaccgac ccgtgcggcg agtgccgggg tcagggccgc gtccagcgcg
    10801 agcggaccct gaccgtccgc atcccggccg gtgtcgacca cgggacgcgg atcaggctcg
    10861 tcggcgaggg cgagggcggc gtcggcggcg ggccgcccgg cgacctgtac gtggtgctgg
    10921 gcgtcgccga cgatccgttc ttcgagcggg acggtctcga tctccattgc gagatcgaaa
    10981 tcggcatggc gcaggccgcg ctcggctcgc gggtcgaggt acggcgcatc gagggcgcgg
    11041 agacggtcga gatcccggcc ggcacgcagt cgggcagcca gatccgtctg cgtggccagg
    11101 gcgtcccggc actcgacggc cgcggccggg gcgaccaggt ggtcaccgtc cgcgttcgca
    11161 cgccgaagcg gttgagcgcc gcgcagcgcg agctgttcga gaagctcgcc gaactggaac
    11221 ggaccgaatc ggaggacccc ggtctgttcg atcgcgtcaa gaagatcttc aactgagcac
    11281 cggccaccat gacggcggag cgatcgagcg gcgacgggga acggtccggc gccgcgggtc
    11341 gcccgcgcgt ctggtcggcc gtcgtcatcg agctgccggc cgcgctggtc gatgaggcgc
    11401 tggcgatcgt cgcgtccgat tcgagcggcg ccgagctggg cgaggacgga gtgctccgcc
    11461 ggctcgccgt ctggttcgag cgggacgagg ccgcggaggg ggcactggcg cgagtccgcc
    11521 gctggctctc gcggcgcggg ctgacggccg acgcccgtct cgaacgggtc gaggacggcc
    11581 actgggccga gcgctaccag gcgggtctgg tgccgttcga cctcggcgcg cgcttcatcg
    11641 tcgaccccag cgggacggag ctcgacgagc cccgggccgg ggtacgaacc tacatccatc
    11701 tcgcgccggg cggcgcgttc ggcaccgggg agcatccgac gacccggctg tgcgtcggtc
    11761 tgttggaatc gctggtcgcc ccgggcaccc gctggctcga tctcggctgc ggaacggcga
    11821 tcctgtccgt cgtcgcccgc tacctcggcg catcgcgcgt ggccgcggtc gacctcgacg
    11881 gcgaggcctg ccaggtcgcg cggcaggttc tcgcgcgcaa cggactgtcg cggcaggtac
    11941 tgctgtggaa cggatcgctg gcggagctcg cgcccgattg ctgggacggc gtcgtcgcca
    12001 acgtcgagct gccgtttttc ctcgagaact ccgcgcggct cgggcgcctg ccggccgtcg
    12061 gcggcttgct gatcgcgagc gggttcctcg gcgacgacgc gcgcctcgtc gcgctgcgtc
    12121 tggggcgcgc cggtctcagc ccgatccgca tcgagatcga tccgccctgg gcggcgctcg
    12181 ccgcacgtcg cgtcgcgcga tgagctcccg acgttggcgc gcccgtcacc ggccgctccc
    12241 cgacacggcg ggtgcgacga tccgcctcga ccgcgatgaa tcgcaccacg tgcaccgcgt
    12301 gctgcgtctg gggcgcggtg cctcgctcgg cgtcttcgac ggtctcggtc tcgagtggga
    12361 cggcgtgatc gacgggatcg acgacggcat cgtcgtcgtc cgcttggtcc gccggctcga
    12421 cgaggtggtc gaaccgccgg tcgagctggt gctgttccag acggtctgcc gcaacgagcg
    12481 aatggagtgg gtgatccaga agacgaccga gctgggcatc gccgcgatcc gtccgatttc
    12541 ggccgagcgc gccgaggcgc gaccaccgac ggccgcgcgg gtcgcccgtt ggcgcaggat
    12601 cgcgctcgag gcggccaagc agtgcggccg gcgtaccgtg ccgggcatcg aaccggccga
    12661 cgcgctcgcc gccccggccg cgggggtgct gccgttgctg ttcgatacgg gccgggattg
    12721 ccggccgctc gccgaacgcc tggtacctct cccggacggt ccggtctggt tggcggtcgg
    12781 ccccgagagc ggcttttccg cggacgagca ctgccgccac gtcggcgagg gctggttgcc
    12841 gaccggtctc gggccccgca cgctgcgcgc ggagacggcg ggaatcgtcg ccgcggcgat
    12901 cgtgctccac ctgtgcggag acctgggccg ggcgagcgac gccggttgac tcgccgatcg
    12961 ctcggactaa attgaccgag gaccgtcgaa tcgtccgggt aggaggtccg atgcacatca
    13021 acgacctgct caagagcgcg accgaccagg gctcgtccga cctgcacctc aaggtcggca
    13081 gctacccggt gatccgcgtc gacgggcggc tgcgtccgat gaccgaccag aagcggctga
    13141 tgcaggaaga cacgatcgcg atggcgttca gcatcatgag cgcccgtcaa aagcagaagt
    13201 tcaaggacca cttcgagatc gacatggcct actccgtgcc ggggttggga cgcttccgcg
    13261 tcaacgtgtt ccaacagcgc gggacggtcg ggttggtgct gcgcgtgatc ccgatcaaga
    13321 tcctctcgat ccgcgagctg cacctgccga ccgtgctcga gcgcatcgcg cacgagcggc
    13381 gcggcctgat cctcgtcacc gggacgacgg gatcgggcaa gtcgacttcg ctggccgcga
    13441 tgatcgactt cgtcaacagc acgcgcaccg agcacatcat gacgatcgaa gacccgatcg
    13501 agttcctgca ccgcgacaag aaaagcctgg tcaaccaacg cgaggtcgag gtcgacacca
    13561 agtcgttcgc gttcgccctg cgctcggcgt tgcgccagga ccccgacgtg attctggtcg
    13621 gcgaaatgcg cgactacgaa acgatcgaga cggcgctgaa cgcggccgag accggacacc
    13681 tcgtgctgtc gacgttgcac accgtcgacg cgaccgagac gatcaaccgg gtgatctcgg
    13741 tcttcccgcc gcaccagcag aagcaggtgc gcctgcagct cggcgccgtg ctccgcgcga
    13801 tcatctcgat gcgactggtg ccgcgcaagg acggcaacgg ccgggtcccc gccgtcgagg
    13861 tgctgcgctc gacggcctac atccgcgact gtgtcgagaa caaagaaaaa accaagctga
    13921 tcgcctccgc catcgcggcc ggtacgtcgc agtacggcat gcagaccttc gaccagtcgc
    13981 tgtacaatct gctgcgggcc gacctgatca gcttcgagga agcgctgcgt caggcgacga
    14041 acccggacga gttcaagctc aaggtggcgg gaatccagtc ggcctccgac atggcgaccg
    14101 aggcgatgga ggcgatgctc cagcagtcgc aggccggcgg cggcacccac aacccgcgcg
    14161 atccggactc gccgttcgag ttctcgaata gctgatagat tgtcgccgtg atcgacggcg
    14221 acgaccgcta cgaccgcgtc gaggcccacg cgctccgcct gctcgcgggg cgcgaccgga
    14281 cggcggtcga gctgcgacgg cacctgacgg ccgccgacca tccgcccgac gaggtcgaac
    14341 gcgtgatcac ccggctgcgg caggccggct acctcgacga tggccgcttg gcgctcgccg
    14401 cgatcctggc ggacgtcgcg gccggtcgcg ccgcggcgcg gatcgatcgt cggctgctcg
    14461 agcgcggggt cgagcccgcg ctgatcgaac gcgcgtggga cgccgccgcg gccgaccacg
    14521 cggtggaccg cgcgcgactg ctcgccgcgg cgctcgagcg gcggctggtc cacgtggcac
    14581 ggccactcga ccggcgggcc attcgccgtg tatataatgc gatgcttcgc gcgggattcg
    14641 acgcggacga ggtcgcggcc gccctggagc cccaccttcc cggcgacgcc ggctgacgcg
    14701 atccgacgac ctccaccgag agccgaacga tgactgccaa cgagacccgc aaacgattcc
    14761 tgagcttctt cgccgatcgc ggtcatcgcg tcgttccgag ctcgccgttg gtcctgccca
    14821 acgatccgac attgctgttc gccaacgccg ggatgaacca gttcaaggat gtcttcaccg
    14881 gacgcgaggt gcgcgattac ctgcgggccg cgagctcgca gaaatgcctg cgcgtctcgg
    14941 gcaagcacaa cgacctcgaa accgtcgggc gtacgccgcg gcaccacacc ttcttcgaga
    15001 tgctcggcaa cttctccttc ggcgattact tcaagaagga ggcggtcgag tacgcgtggg
    15061 agctcgtgac cggcgtcttc gggttgcccg aggagcgttt gtgggccagc gtgttcggcg
    15121 gcaccgcgac cctgccggcg gacgaggagg ccttccgcct gtggaccgag acgatcggcc
    15181 ttccggccga acgcgtactt cggttgggcg agaaggacaa tttctggcgc atgggcgaga
    15241 ccggcccgtg cgggccgtgc agcgagctgc acttcgacct gggcgccgac ctggcatcgg
    15301 tcgaacccgg ctcgaacccc gcgaccgacg accggaggtt cctcgagatc tggaacctcg
    15361 tcttcatgca gttcgatcag cagagcgacg gagcgctggt gccgcttccc gcgccgagca
    15421 tcgacacggg gatggggctc gagcgcatcg cctcggtgct gcagggcaag cgcagcaact
    15481 acgataccga cctgtttcgg ccgattctgc aggcggtcgc cgagcgggcc gcggtacgct
    15541 acggcgcgga cgaggaaacg gatctggcgc tacgcgtgat cgccgatcac ggtcgcgcgt
    15601 tctgcttctt gaccgccgac ggcgtggtac cggccaacga caaccgcgga tacgtcctgc
    15661 gccgcctgct gcgccgcgcc atccggttcg ggcgcaagct gggcatcgac gaaccgttcc
    15721 tccatcacgt cacgccggcg gtggtcgacg ggttgggcga tcactacccc gagttactgg
    15781 ccgcccggga tgcgatgctc gagatcggca agctcgaaga gcagcggttc gcggacacgt
    15841 tgtccaccgg actccaggtg ctcgagcagg cgttggcggg caccgagccg gatcgatcga
    15901 ccgggcggcc ggtgctggcc ggtgccgacc tgttccggct gtacgacacc ttcggcttcc
    15961 cgctcgacct ggcgcgggac gtcgctgacg aacgcgggat cgagctggac gtggccggtt
    16021 tcgaacggga gatggccaag caacgcgcgc gggcgcaggc gtcgtggaag cgcgtcaaga
    16081 acgagcgggc gagcggcgtc tacagcgagc tggtcgaccg ggtccagagc cggttcgccg
    16141 gctactccgt cgagcgtctc gacggcgtgc cggtcgccgc ggtcgtccgc gacgggcggc
    16201 cggtcgacgt gctcgagggc ggcgaggaag gcgaggtggt gctcgagctg acgccgttct
    16261 acgccgaggc cggcgggcag gtggccgata ccggtttcct cgcggcgccg tcggggcacg
    16321 ccgaggtcat cgacgtccag cgcccgctgc ccgggctgat cgtccatcgc gtgcggctgg
    16381 tgtcgggcgc gctcgagaac cgggaacgcg tgacggccga gatcgacacg cgcaggcgcg
    16441 aggcgacgcg acgcaaccac acggcgaccc acctgctgca cgccgcgctg cgcgaggtcg
    16501 tcggcacgca cgtcaagcag gccggctcgc tcgttgcgcc cgaccgactg cgtttcgact
    16561 tcagccactt cgcggggctt gccgatcacg cgctggccga cgtcgagttg ttgatcaacc
    16621 gcaaggtgat ggaggacctc gcggtcgaga ccgacgagat ggatctcgac gacgcgatcg
    16681 ccgcgggggc gatggcgctc ttcggcgaga agtacggcga gcgggtccgc gtcgtgcgga
    16741 tcggcgactt ctcgctcgag ctgtgcggcg ggacccacac ggcgcgaaca ggcgagatcg
    16801 gactggtcaa gctcctgcac gagcgcggcg tcgcctccgg aacccggcgc gtcgaggcgg
    16861 tcagcggcga gggtgcgctg tcggccttcg gcgacgacca gcgggtggtc cgcgccct
//
LOCUS       DASPGN010000067         3419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_949217, whole genome shotgun sequence.
ACCESSION   DASPGN010000067 DASPGN010000000
VERSION     DASPGN010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949217"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VD788_01980"
     CDS             <1..322
                     /locus_tag="VD788_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXV75059.1"
                     /translation="IFTERDALYRACDGDGVTADRARRTLVREVMSQPVKTVRRQQSL
                     AEAIERMVREGCRHLVVVDRNGALKGLLTTNDLIQFVTERFPEETVNLPPRLHQRYLT
                     PEGA"
     gene            327..866
                     /locus_tag="VD788_01985"
     CDS             327..866
                     /locus_tag="VD788_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXV75060.1"
                     /translation="MICPACDHDNIAGVDQCADCGTDLAGLEAPMWDLDPNDALLARP
                     LEQLPLKPALVVRSGSSVADAIELMCERREGCVFVVDPRDRLVGVLTERDVSTRVVVR
                     GRDPRSLPIDQVMTREPVTMRRDDPLAWALHRMGVDGHRHLPVVDGGRLLGFVSARRV
                     LGAFLDAPAGGVTGRGAAE"
     gene            899..1735
                     /locus_tag="VD788_01990"
     CDS             899..1735
                     /locus_tag="VD788_01990"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HXV75061.1"
                     /translation="MRLPLARMLTMAVLSGALSAASTPTVPSAFVDPGELRVRENLVE
                     RPGRELFDWGRERLAELGVAGLPATDQATRIARYIHDRYAFTPDRPPDLATFVADAAG
                     NCYAHARLGAFLLRLAGIPTRFVYEVHLERKTPQASADARERGIGLFGQYHNDHFWLL
                     YHDGARWVPFDSTLGIADYDEFVRVKIDSPQGGVANPPFLLWREPAVAGAPMENFTGT
                     FWREIGRETLPRVTQADWQALLDTVGRLALADLMRPLRPDHEQAIGRVGRAFFGAGGT
                     PR"
     gene            complement(1732..>3419)
                     /locus_tag="VD788_01995"
     CDS             complement(1732..>3419)
                     /locus_tag="VD788_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXV75062.1"
                     /translation="ARGRSRIVSDGELLRCFRAFLLLARQFGVDRGGGEPALAELEVN
                     PFAFRQQQLVPLDGRGRLGPAPRALPARPPAEVERLLEPRSIAVLGVSASAVNFGRII
                     LRNVIGSGFPRERIRVIKRGRAEIDGVRCLPQIASLPEPVDLLVIAASSRQLPQIVRE
                     TIASGRVRAAIMIPGGVGETERSGEIAAQVRETIAEVRRRGDGGPVFLGPNSLGLVSR
                     PGSYDTLFIPPSRLDKRWAAPARPVALVSQSGAFIVSRMSNLETLDPAFAVSLGNQID
                     VTASDLVAALAERDDVQTIGVYAEGFPDLDGLEFVRQVRRVSGAGKCVVFYKAGRTEP
                     GRNAVAGHTATVAGDYEVCQAAVEQAGGLVTDTFKEFEQLVELSTALHRKRVAGVRVG
                     VVSNAGFETVGMADAIQGQRYRVQIAPLGGDSVRRLAEVLDRHGLGPLVNARNPLDVT
                     PMAGERVYEDVVGVMMDCDTVDAVVVGIVPLTASLATAPDEIEKPASLARRLPELFGR
                     YDKPLVAVVDSGPLYDPFVRALRVGGVPVFPSADQAIRSLGRYLCHRVESSRG"
BASE COUNT          501 a         1241 c         1200 g          477 t
ORIGIN      
        1 catcttcacc gagcgcgacg cgctctaccg tgcgtgcgat ggcgacggcg tcaccgccga
       61 ccgggcgcgg cggacgctgg tgcgagaggt gatgagccag ccggtcaaga cggtgcggcg
      121 gcagcagtca ctggcggagg cgatcgagcg gatggtgcgc gagggttgtc gccatctggt
      181 ggtcgtcgat cgcaacggcg cgctcaaggg gctgctgacg acgaacgatc tgatccagtt
      241 cgtcaccgag cgttttcccg aggagacggt caacctgccc ccgcggctgc accagcgcta
      301 cctgactccc gagggggcgt gagcggatga tctgcccggc ctgcgaccat gacaacatcg
      361 ccggcgtcga ccagtgcgcc gactgcggca cggacctggc agggctcgaa gcgccgatgt
      421 gggacctcga tccgaacgac gcactgctcg cccgcccgct cgagcagctg ccgctcaagc
      481 ccgcgctcgt cgtgcggagc ggcagctcgg tggcggacgc gatcgagctg atgtgcgagc
      541 ggcgcgaggg ttgcgtgttc gtcgtcgatc cacgtgaccg tctggtcggc gtgctgaccg
      601 agcgcgacgt cagcacgcgg gtcgtcgtcc gcgggcgtga tccacgttcg ctgccgatcg
      661 accaagtgat gacgcgcgag ccggtcacga tgcggcgcga cgatccgctg gcctgggcgc
      721 tccaccggat gggggtcgac ggccaccgcc acttgccggt cgtcgacggc gggcggctgc
      781 tcggcttcgt ctccgcgcgt cgcgtcctcg gagccttcct cgacgcgccg gccggcggtg
      841 taaccggacg aggtgccgcg gagtagaaac cggtgtcccc tgccgggagg aggcgccgat
      901 gcgactgccg ctcgcccgca tgctgacgat ggccgtgctg tccggggcgc tgtcggccgc
      961 ctcgaccccc acggtgccgt ccgccttcgt cgacccggga gagctgcgcg tgcgggagaa
     1021 cctggtcgaa cgccccggac gcgagctgtt cgactggggc cgggagcgac tggccgagct
     1081 cggcgtcgcc ggactacccg caaccgacca ggcgacgcgc atcgcccgtt acatccacga
     1141 ccgctacgca ttcacaccgg atcgaccgcc cgacctcgcg accttcgtcg ccgacgccgc
     1201 gggcaactgc tacgcacacg cccggctcgg ggcattcctg ttgcgcttgg ccggtatccc
     1261 gacgcgcttc gtctacgagg tgcacctcga gcgcaagacg ccccaggcct ccgccgacgc
     1321 ccgcgagcgc gggatcgggc tgttcggtca gtaccacaac gaccacttct ggctgctgta
     1381 tcacgacggc gcgcgctggg tccccttcga ctcgaccctg ggcatcgccg actacgacga
     1441 gttcgtccgg gtcaagatcg actctccgca gggcggcgtc gccaacccgc cgttcctgct
     1501 gtggcgcgag cccgcggtcg ccggcgcacc gatggagaac ttcaccggga cgttctggcg
     1561 cgagatcggc cgcgagacgt tgccccgcgt gacgcaggcc gattggcagg cgttgctcga
     1621 caccgtcggg cgtctggcgc tggccgacct gatgcggccg ctgcgacccg accacgagca
     1681 ggcgatcggg cgcgtcgggc gggcgttctt cggcgccggc ggtacgccgc gctagccgcg
     1741 gctcgattcg acccgatgac acaggtaacg gccgagcgag cgaatcgcct ggtcggcgga
     1801 ggggaagacc gggacgccgc ccacccgcag cgcacgcacg aacgggtcgt agagcggccc
     1861 ggagtcgacg accgcgacca gcggtttgtc gtagcgaccg aacagctccg gcaggcgtcg
     1921 cgccagcgac gcgggttttt cgatctcgtc cggcgccgtc gccagcgacg cggtcaacgg
     1981 cacgatgccg acgacgaccg cgtcgacggt gtcgcagtcc atcatcacgc cgacgacgtc
     2041 ctcgtagacc cgctcgccgg ccatcggcgt cacgtccaac ggattgcgtg cgttgaccag
     2101 cggccccagt ccgtgccggt cgagcacctc ggccagccgt ctcacggaat cgccaccgag
     2161 cggcgcgatc tgcacccggt agcgctgtcc ctggatcgcg tcggccatgc cgaccgtctc
     2221 gaacccggcg ttcgacacca cgccgacgcg caccccggcg acccgcttcc ggtgcagcgc
     2281 ggtcgacagc tcgaccagtt gctcgaactc cttgaacgta tcggtgacga gccccccggc
     2341 ctgctcgacg gcggcctggc agacttcgta gtcaccggcg accgtcgcgg tgtgcccggc
     2401 gacggcgttc cgccccggct cggtccggcc cgccttgtag aagacgacgc acttgcccgc
     2461 cccgctgacc cggcgcacct ggcgaacgaa ctcgagtccg tcgagatcgg ggaatccctc
     2521 ggcgtagacg ccgatggtct gcacgtcgtc gcgctcggcg agggcggcca cgaggtcgct
     2581 cgcggtcacg tcgatctggt tgccgagcga cacggcgaat gcgggatcga gcgtctcgag
     2641 attgctcatc cggctgacga tgaacgcccc gctctgcgag acgagcgcga ccggacgggc
     2701 cggcgccgcc caacgcttgt cgagtcgcga cggcgggatg aacagggtgt cgtagctgcc
     2761 cggccgtgaa acgaggccga ggctgttcgg cccgagaaag accgggccgc catcgccgcg
     2821 ccgccggacc tcggcgatcg tctcgcgcac ctgggccgcg atctccccgc tgcgctccgt
     2881 ctcgccgacg ccgcccggga tcatgatcgc cgcacggacc cggccggagg cgatcgtctc
     2941 gcggacgatc tggggcagct ggcgcgagga ggcggcgatc accagcagat cgaccggttc
     3001 cggcagcgaa gcgatctgcg gcagacagcg cacgccgtcg atctcggcac gaccgcgttt
     3061 gatcacgcgg atccgctcgc gcgggaagcc cgagccgatc acgttgcgca ggatgatccg
     3121 gccgaagttg acggcgctgg ccgacacgcc gaggacggcg atcgatcgcg gctcgagcag
     3181 ccgctcgacc tcggccggcg gacgcgccgg cagggcgcgc ggcgccggcc ccagccgccc
     3241 gcgtccgtcc agcggcacga gctgctgctg ccggaaggcg aacgggttga cctcgagctc
     3301 ggccagcgcc ggctcgcctc ccccgcgatc gacgccgaat tgcctggcca acaacaggaa
     3361 ggcgcggaag cagcgcaaca gctcgccgtc cgagacgatc cggctccgtc cgcgggccc
//
LOCUS       DASPGN010000068         6725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_832702, whole genome shotgun sequence.
ACCESSION   DASPGN010000068 DASPGN010000000
VERSION     DASPGN010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6725
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832702"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VD788_02000"
     CDS             complement(<1..705)
                     /locus_tag="VD788_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV75063.1"
                     /translation="MPHAGPRPDHPHESFPERALDGTIDARFAAIVARHGERTAVEDG
                     ARSLSFRDLDQAAARVAAALAAARGPAGDDSRPVALLLEQGADFISALIGVLRAGRFA
                     VPLDPRNPASRNRLILGDSTATAIVASARTSELARALSAGVPPILLDALREPNQAPKR
                     VDRTADAEAILLYTSGSTGRPKGVIQTHRNLLHNTLRHTNEFRIVPGDRFSLLYPCGV
                     YGGVRDIFNALLNGACL"
     gene            complement(718..1977)
                     /locus_tag="VD788_02005"
     CDS             complement(718..1977)
                     /locus_tag="VD788_02005"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXV75064.1"
                     /translation="MSRRLTYERVRLPLLMRACNRVARGAARLGLAAAPMRAESILRR
                     ATKRSGLADFGDGDVGEPLAVLVDSLEHEAALTPLGHKSIEFALVETLAKRLRIVDHV
                     ARHPAVGRQTIERPVFVVGFPRSGTTLLFNLLAQDPAARPLLGWEASEPLPRRPPRPG
                     RRDPRIRRHRRSLRRLDHIVPELRKVHELTAEGPEECNLLLMRSLVTWFYGLLTRVPS
                     YERWLASRPRSTFNAAYRLHRAQLQALQHQRRGERWLLKSPLHLNTLDALVEVYPDAC
                     IVQTHRDPAKVHPSSCSLIALTRSIFSDRIDPVAIGREALDEGLRRVDRLLAMRETLS
                     ADRVYDVRYAELTDDPPRTLRAIYDHFGLSWPDGMERRVRGWLASSPRHKHGVHRYAL
                     EAFGTTAAEIDRLSSGYRERFGVPRER"
     gene            complement(1974..6659)
                     /locus_tag="VD788_02010"
     CDS             complement(1974..6659)
                     /locus_tag="VD788_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HXV75065.1"
                     /translation="MNPRDVVVVGMACVFPGADDVATFWRNVVAGKDAIGDVPAGRWP
                     GSRLTELPPDHEGHVGCRRGGFISTPYLFDPLRHRVMPRVARHGDVDQFILLDVIADA
                     LADAAIGEGAACRATTDVILGRGNYTSNKMLEVFLRSDGCDRILAFLGRRLPQLTDAD
                     LAALERELRASLPPRDPDGMATAITNLTASRAANRLDLGGAAFVVDAACASSLVAMEI
                     GVERLREGKCDVAVVAGINFTNVPGFWYLFSQLGAFSPSGQIRPFDRRADGILIGEGA
                     GAVVLKRAERARRDGDRVYALVKGLGSASDGKAAGVLAPSSGGQVRALERAYHDAGLE
                     PEVIGLLEAHGTGTPTGDRVEIETLRRVFGPPLAGVPTRVMGSVKSMIGHAMPAAGIA
                     SFIKVALALTGKILPPSLHCDQPEPALAELPFFLSPVARPWLQSPKDGPRRAAVNAFG
                     FGGINAHVIVEEVLPENGRPTSVAVPGPPAADLPPLRPRPLRPGIRPPTELLAFSADD
                     RPALAGRLRQVARFLECEGRPFDLADLSHTLAAETDTRARCRLALIRPGLDGLAGELV
                     KLAARVEGGAGIATAEGAGLYYGDGEATPAGHIAAVFPGIAFPGLTGEFPDHLLTNCM
                     HFPVAREVFDLVERRDEHPDDPLPTSLILVPPPHLDESRRARIRNRFAPAAAVPEGQL
                     DRSPPAERNLSHMGTLVNNWAGWQILRSLDIEIDMLCGQSLGDLSAVLSAGMADFEES
                     IPNFWKAFNLEIPYVGSGAILMVGAAEEQLTPLLDGLADVSIAIHLSPSVLVVGGSER
                     GIGLLAERLRGASIFSQKLPFPPIHTPRLLEFQEALAAAMGETAPLRPARVTIYSAVL
                     AAPMPDDPEQVAELLRSNVSRPIRFWQTQRRMVDDGARFIVQIGSGTLAASSRAVLDR
                     SDVKCIAMDVGERHPVTQIQTLCAQLFADGLQLKLDGLFAARQPRMLALTAPAPADPP
                     SPTALPLTLYWPPLWADEEAPAGPAPVAASEPATMLPFLGRVERFEPGRRIAAVTRLE
                     LDEHRYLAHHVFLNITGVKSLRDCGPVLPLTFTLEMLAETAACLAPGLGLIGLTRVRA
                     RRWIGFDGVDAIDVRVEAELDGADADCLSISARATFDGEVAAEAVIRFAARYRQTIDL
                     AFHQPVNPRRFPLTAKQVYDGRYFFHGPLFHCVEALDVMSDRGATGRMVVAASRGLFR
                     SLPNPQLLLDPVALDGAGQVVGAHFLERDISLLPVSVDAIEFYRPAPAPGTRTPVHVE
                     FTEIDLEGRRAKSNIEVQDGEGRVWFRVLGWQDVLYLCRPSWTESQRRPERATLSTRC
                     EIDGVPADVVVMRVTREDVRHVPPAWLAKSFLHADEERRVPWGAGQDPRTHLPWILGR
                     VAAKDAARAWRARTAPGSPMAHPVEVVLANDGAGRPHVEAIAGAGPVPVISLSHGEAG
                     AVAIAADRPVGIDVEAIDAGAALTLADFATDAERGLLERLAGMTDDPSRVTRLWCAKE
                     AAAKLVGSGLGGRPKEFEAVDADSRGRLTIAHRPTGRRIDVRTSVDDRLLLAVAVAAT
                     GVAPGRAGR"
BASE COUNT          986 a         2403 c         2442 g          894 t
ORIGIN      
        1 gaggcaggcg ccgttgagca gcgcgttgaa gatgtcgcgc acaccgccgt agaccccgca
       61 cggatagagc agactgaagc ggtcgccggg gacgatgcga aactcgttgg tgtgccgcag
      121 cgtgttgtgc aggaggttgc ggtgggtctg gatcacgcct ttcggccggc cggtcgaccc
      181 ggaggtgtag agcaggatcg cctccgcgtc cgcggttcgg tcgacccgct tcggtgcctg
      241 gttcggctcg cggagcgcgt cgagcaggat cggcggcacg cccgcggaga gcgcgcgggc
      301 gagctcgctc gtccgcgcgc tggcgacgat cgccgtcgcg gtcgaatcgc cgaggatcag
      361 ccgattgcgt gaggcgggat tgcgcgggtc gagcgggacg gcgaaccgtc cggcgcgcag
      421 gacgccgatc agcgcactga tgaaatcggc gccctgctcg agcaacagcg cgaccggccg
      481 cgagtcgtcg ccggccgggc cgcgcgccgc ggccagcgcc gccgccacgc gcgcggcggc
      541 ttgatccagg tcgcggaagc tcaagctgcg cgcgccgtcc tcgaccgcgg ttcgctcgcc
      601 gtgccgtgcg acgatcgcgg cgaacctcgc gtcgatcgtg ccgtcgagcg cccgctcggg
      661 gaacgactcg tgagggtgat ccggtcgagg tccggcgtgc ggcacggcag cctccgctca
      721 acgctcccgc ggcacgccga agcgctcgcg atagccggag gacagtcgat cgatctcggc
      781 cgccgtcgtg ccgaacgcct cgagcgcgta gcggtgcacg ccgtgcttgt gccgggggct
      841 gctcgccagc caaccgcgca cgcgccgttc catcccgtcg ggccagctca gtccgaagtg
      901 gtcgtagatc gcgcggagcg tccgcggcgg atcgtccgtc agctcggcgt agcgtacgtc
      961 gtagacgcga tcggccgaca gcgtctcgcg catcgcgagc aatcgatcga cccgccgcag
     1021 tccctcgtcc aacgcctcgc gcccgatcgc gaccgggtcg atccggtcgg agaagatgct
     1081 gcgcgtcagc gcgatcaggc tgcagctcga tggatggacc ttcgccgggt cgcggtgggt
     1141 ctgcacgatg caggcgtccg ggtagacttc gaccagcgcg tcgagcgtgt tgaggtgcag
     1201 cgggctcttg agcagccatc gctcaccgcg gcgttggtgc tggagcgctt ggagctgcgc
     1261 acggtgcagg cggtaggccg cgttgaaggt cgaccgcggc cgcgaagcca gccaccgttc
     1321 gtagctcggt acgcgtgtca gcagtccgta gaaccaggtg acgagcgatc gcatcaacag
     1381 caggttgcac tcctcggggc cctcggccgt cagctcgtgc accttgcgca gctcgggaac
     1441 gatgtggtcc aaccgccgca ggctgcggcg atgacgccgg atgcgcgggt cgcggcggcc
     1501 gggccgcggc ggccggcggg gcagcggctc cgacgcctcc caacccaaaa gcggtcgggc
     1561 ggcagggtcc tgcgccagca ggttgaacag caacgtcgtc ccgctgcgcg ggaagccgac
     1621 gacgaagacc gggcgttcga tcgtctgtcg accgaccgcc ggatgccgcg cgacgtggtc
     1681 gacgatgcgc agccgcttgg cgagcgtctc gaccagtgcg aactcgatgc tcttgtggcc
     1741 gagcggcgtc agcgccgcct cgtgctccag cgagtcgacc agcaccgcga gcggttcgcc
     1801 gacgtcgccg tcgccgaagt cggcgagccc acttcttttc gtcgcccgcc gcaggatcga
     1861 ctcggcgcgc atcggcgcgg ccgccaggcc caggcgagcc gccccgcgcg ccacgcggtt
     1921 gcaggcgcgc atcaacagcg gcaggcgcac gcgctcgtag gtcaatcgcc ggctcatcga
     1981 ccggcacgac ccggcgccac accggtcgcg gcgaccgcca ccgccaacaa caggcggtcg
     2041 tcgaccgacg tgcgcacgtc gatccgccgg ccggtcgggc ggtgcgcgat cgtcaggcgt
     2101 ccgcgcgagt cggcgtcgac cgcttcgaat tccttcggtc ggccgccgag accgctgccg
     2161 accagcttgg cggcggcctc tttcgcgcac cacagccgcg tcacgcgcga cggatcgtcc
     2221 gtcatcccgg caaggcgctc gagcagaccg cgctcggcgt cggtcgcgaa gtcggcgagc
     2281 gtcagcgcgg cgcccgcgtc gatcgcctcg acgtcgatcc cgaccggacg gtcggcggcg
     2341 atcgccaccg cgcccgcttc gccgtgggac aggctgatca ccggcacggg gcccgcgccg
     2401 gcgatcgcct cgacgtgcgg gcggccggcg ccgtcgttgg ccagcacgac ctcgaccgga
     2461 tgggccatcg gcgagccggg ggccgtccgc gcgcgccatg cgcgtgccgc gtccttcgcc
     2521 gccacccgac cgaggatcca cgggaggtgg gtccgcggat cttggcccgc accccagggc
     2581 acgcgtcgtt cttcgtccgc gtgcaggaac gacttggcca gccacgcggg agggacgtga
     2641 cgtacgtcct cgcgcgtcac gcgcatcacg accacgtcgg ccggaacgcc gtcgatctcg
     2701 cagcgggtgg agagcgtggc tcgctcgggt cgccgctggc tctcggtcca gctcggccgg
     2761 cagaggtaga gcacgtcctg ccagccgagc acgcggaacc agacccggcc ttcgccgtcc
     2821 tgcacctcga tgttcgactt cgcccgccga ccctcgaggt cgatctcggt gaactcgacg
     2881 tgcaccgggg tccgcgtgcc cggggccggc gccgggcggt agaactcgat cgcgtcgacg
     2941 ctgaccggca gcagactgat gtcgcgctcg aggaaatggg cgccgaccac ctgacccgcc
     3001 ccgtcgaggg cgaccggatc gagcagcagc tgcgggttcg gcagcgagcg gaacagaccg
     3061 cgcgacgccg ccacgaccat ccgacccgtc gcgccgcgat cgctcatcac gtcgagcgcc
     3121 tcgacgcagt ggaacagcgg tccgtgaaaa aaatagcggc cgtcgtagac ctgcttggcc
     3181 gtcagcggaa agcggcgcgg attgaccggc tggtggaagg cgaggtcgat cgtctgccgg
     3241 taacgcgccg cgaaccggat caccgcctcg gccgccacct cgccgtcgaa ggtggcccgc
     3301 gccgagatcg acaggcaatc ggcgtcggcg ccgtcgagct cggcctcgac gcgcacgtcg
     3361 atcgcgtcga caccgtcgaa accgatccag cggcgagcgc gcacccgcgt cagcccgatc
     3421 agaccgaggc ccggtgcgag acaggccgcc gtctcggcca gcatctcgag cgtgaaggtc
     3481 agcggcagca ccggcccgca gtcgcgcagc gacttgaccc cggtgatgtt gaggaagacg
     3541 tggtgcgcca ggtaacggtg ctcgtcgagc tcgagtcggg tgacggcggc gatgcgtcgg
     3601 cccggctcga agcgctcgac gcggccgaga aacggcagca tcgtcgccgg ctcggacgcc
     3661 gcgaccggcg cgggcccggc gggtgcttcc tcgtcggccc acagcggcgg ccagtacaac
     3721 gtcagcggca gcgcggtcgg cgacggcggg tcggccggcg cgggcgcggt caacgcgagc
     3781 atccgcggct gccgcgcggc gaacaggcca tcgagcttca attgcaggcc gtcggcgaac
     3841 aactgggcgc acaacgtctg gatctgcgtc accggatgcc gctcgccgac gtccatcgcg
     3901 atgcacttga cgtcggagcg gtcgagcacc gcgcggctgc tcgccgccag cgtgccggag
     3961 ccgatctgga cgatgaaccg cgcaccgtcg tcgaccatcc gccgctgcgt ctgccagaaa
     4021 cggatcggtc gcgacacgtt cgagcggagc agctcggcga cctgctccgg gtcgtccggc
     4081 atcggcgcgg cgaggacggc ggagtagatc gtgacccgcg ccggtcgcag cggcgccgtc
     4141 tcgcccatcg cggcggcgag cgcctcctgg aactcgagca gccgcggcgt gtggatcggg
     4201 gggaacggca gcttctgcga aaagatgcta gcgccccgca ggcgctcggc gagcaggccg
     4261 atgccacgct ccgagccgcc gacgaccagc accgacggcg acaggtggat cgcgatcgac
     4321 acgtcggcca gcccgtcgag cagcggggtc agctgctctt cggcggcgcc gaccatcagg
     4381 atcgcgccgc tgccgacgta ggggatctcg aggttgaacg ccttccagaa gttggggatg
     4441 ctctcctcga aatcggccat cccggccgac agcaccgccg acaggtcgcc gaggctctgc
     4501 ccgcacaaca tgtcgatctc gatgtcgagg ctccgcagga tctgccagcc ggcccagttg
     4561 ttgaccagcg tgcccatgtg cgacaggttg cgttccgccg gcggagaccg gtcgagctgg
     4621 ccctcgggta ccgcggcggc cggtgcgaag cggttgcgga tcctggcgcg gcggctctcg
     4681 tcgagatgcg gagggggcac gaggatcagg ctggtcggca gcggatcatc cggatgctcg
     4741 tcgcgtcgct cgacgagatc gaacacctcc cgagcgacgg ggaagtgcat gcagttggtc
     4801 aacagatggt cggggaactc cccggtcaga ccggggaagg cgatgccggg aaagaccgcg
     4861 gcgatgtgac ccgccggcgt ggcctcgccg tcaccgtagt acagccccgc gccctcggcg
     4921 gtcgcgatcc cggcaccgcc ctcgacgcgg gcggccagct tgacgagctc gccggccagt
     4981 ccgtcgagcc ccggtcggat cagcgccagc cggcagcggg cgcgggtgtc ggtctcggcc
     5041 gcgagcgtgt gcgacaggtc ggccaggtcg aacgggcgcc cctcgcactc gagaaaccga
     5101 gcgacctggc gcagtcggcc ggcgagcgcc ggccgatcgt cagcggagaa ggccagcagc
     5161 tcggtcggcg ggcggatgcc cgggcgcaac ggcctcggcc gcagcggcgg caggtcggct
     5221 gcgggcggac cgggcacggc gacgctcgtc gggcgtccgt tctccggcaa gacctcctcg
     5281 acgatgacgt gggcgttgat gccgccgaaa ccgaacgcgt tgaccgccgc gcggcgcgga
     5341 ccgtctttcg gcgactgaag ccacgggcgc gcgaccgggc tcaggaagaa cggcagctcg
     5401 gcgagcgccg gttccggctg gtcgcagtgc aggctcggcg gcaggatctt gccggtcaac
     5461 gccagcgcga ccttgatgaa cgacgcgatc ccggcggccg gcatcgcgtg accgatcatc
     5521 gacttgaccg agcccatcac gcgggtcggc acgccggcca gcggtgggcc gaagacccgc
     5581 cgcagcgttt cgatctcgac gcggtcgccg gtcggtgtcc cggtgccgtg ggcttcgagc
     5641 agcccgatca cctccggctc gagcccggcg tcgtgatagg cccgctcgag cgcccgcacc
     5701 tggccgccgc tcgacggcgc gagcacgccg gccgccttgc cgtcgctcgc gctgcccaac
     5761 cccttgacca gcgcgtagac ccggtcgccg tcgcggcgcg cccgttccgc gcgcttcagc
     5821 accacggcac cggcgccctc gccgatcagg atcccgtcgg cccgccggtc gaacgggcgg
     5881 atctgtccgc tcggcgagaa cgctccgagc tgcgagaaca gataccaaaa cccgggaacg
     5941 ttggtgaagt tgatcccggc gacgacggcg acgtcgcact ttccctcgcg cagacgctcg
     6001 acgccgatct ccatcgcgac cagcgacgag gcacaggccg cgtcgacgac gaacgccgcc
     6061 ccgcccagat cgagccggtt ggccgcgcgg ctcgccgtca ggttggtgat cgccgtcgcc
     6121 atcccgtcgg gatcgcgcgg cggcaggctc gcgcgcagct cccgctcgag cgcggccagg
     6181 tcggcatccg tcagctgcgg caggcgccgg ccgaggaagg cgaggatgcg gtcgcagccg
     6241 tccgagcgca ggaagacctc gagcatcttg ttcgaggtgt agttgccgcg accgagtatc
     6301 acgtcggtgg tcgcgcggca cgccgcgccc tcgccgatcg ccgcgtcggc cagcgcgtcg
     6361 gcgatgacgt cgagcaggat gaactggtcg acgtcgccat gtcgcgcgac gcgcggcatg
     6421 acgcggtggc gcagcggatc gaacaggtac ggcgtggaga tgaagccacc gcgccggcag
     6481 ccgacgtggc cctcgtggtc gggcggtagc tcggtgagac ggcttcccgg ccagcgcccg
     6541 gcgggcacgt ccccgatcgc gtccttgccc gcgacgacgt tgcgccagaa cgtggccacg
     6601 tcgtcggcgc cgggaaagac gcacgccata ccgacgacga cgacatcccg cgggttcatc
     6661 gctcccgtgt ttcgtaggtc gcgcgcgcca tccgccgggc attgcaggcc agcacgacct
     6721 ccggc
//
LOCUS       DASPGN010000069         6293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_949407, whole genome shotgun sequence.
ACCESSION   DASPGN010000069 DASPGN010000000
VERSION     DASPGN010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6293
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949407"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VD788_02015"
     CDS             <1..1107
                     /locus_tag="VD788_02015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV75066.1"
                     /translation="SALALPWLVKFLWAPLVDRYGSVRFGHYRSWIVPLQLLTVLAVV
                     ALSRVDLGRGLTGLLVAAGAFMLCSATQDIATDGLAVRSLRREERGLANGIQVGGYYL
                     GQILGGGLILIVFSRFGWSAAVLSMALFLALPLWPTTRFREPGHPPGRTPGVRVDWKA
                     LGRFATRSGAGVWILILLLYRSGEAMALTMVNPMLVDLGLTLDEIGLLLGLAGSLASF
                     AGAVGGGLWIARVGRRRSLVVFGLLQAVALGGYLLPASGFTWLPAIYAVTMTAACCSG
                     MATAALYTNMMDRCDPATGATDFTLQQSLCAVGPLVAASLSGFSAASLGYAMHFGMTV
                     AVALIAVGVVAAGLTEASGAPRPAVGAVSTAADA"
     gene            1165..2829
                     /gene="asnB"
                     /locus_tag="VD788_02020"
     CDS             1165..2829
                     /gene="asnB"
                     /locus_tag="VD788_02020"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF006949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HXV75067.1"
                     /translation="MCSILCVFDIQHDAAALRARALELSRRLRHRGPDWTGIFSSERA
                     ILAHERLAIVDVEHGAQPLESGDGRYVLAVNGEIYNHAALERALARPYEFRTKSDCEV
                     ILPLFAQRDTGFLDELNGIFAFAIYDRDEDRYLLARDPIGVMPLYYGHDEHGRVWVAS
                     EMKGLIDVCRSFAEFPPGHYLDSRAGEPVPYYRPVWRDYAAVRGQAPDLARLRTSLEA
                     AVERQLMCDVPYGVLLSGGLDSSILSAVCHKFYKTRVENGGRSEAWWPQLHSFAIGLV
                     GSPDLAAARKAAAAIGTIHHEHTYTIQQGLDAVSDVIYHLETYDVTTVRAATPMFLLA
                     RRIKAMGIKMVLTGEGSDEIFGGYLYFHKAPDAEEFHAETLRKLDQLHLFDCLRANKA
                     LAAWGVEGRVPFLDREFLDVAMGFDACHKMCGGDRIEKHVLREAFAGYLPQEILWRQK
                     EQFSDGVGYAWIDTLKRVAEEQIGDQQFARAARRFPSHVPATKEAYLYRAIFEGHFPG
                     EAAAATVPHGMSIACSTPTAIRWDEMFRTRADPSGRAMKGVHRDAF"
     gene            2933..3328
                     /locus_tag="VD788_02025"
     CDS             2933..3328
                     /locus_tag="VD788_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75068.1"
                     /translation="MRDSTKQTPVWARTLDTIAARIEQAPAARKRDLDPGDRVIVATR
                     NSVYCLQALGGDLFEVSGGWFERNPDSRLITVNGCTYGGLAIHTDIVAARGLFLEFGN
                     NVSTTRIQRVLVVRGNRQSAAAGHSNLPN"
     gene            complement(3710..5140)
                     /locus_tag="VD788_02030"
     CDS             complement(3710..5140)
                     /locus_tag="VD788_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75069.1"
                     /translation="MSRIRRVAVVLCAIALLLPGSVLAVTREIADERDSPLTFRRSVI
                     NQLTHDLFWDPYDELGRRPFNLPFVNIDRHGNLSPWPDQEGDHRHYVNALVGNNGNTN
                     VQQGSDVFSGAYIRQPGSAFNWGISASLFADDNENTGTNPVDSIRVTNSLTGYDLRFA
                     AAYVLNESFTIGGGIDLSGRDDEETASSFELGIGGFNGTDEVTRSGFGFDVGVRHFLD
                     ERSSWQAQLIYGNGSTEQNEFAESLDDTGAIVARTDILNYDITDTTIVLGGAYNRRFQ
                     ALRGEWQAGLTLGQIESSLDNSDLAFDEGGPLVTLLGQDAVTETSIVASFGSINLAGP
                     STQVFTGAELTLSTLDGSTEIGDGVNTSSESIDDTLTALGLKLGIRQPLGTDKLRLIA
                     STRADFLDREMKDVFDASTSTDGSTRTTTEYAIGVEGVFLNMTFDLAWRFGEEVALSP
                     APGESTGLSRRVIEFDRLIAAAIFAW"
     gene            complement(5186..>6293)
                     /locus_tag="VD788_02035"
     CDS             complement(5186..>6293)
                     /locus_tag="VD788_02035"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HXV75070.1"
                     /translation="GTLVEPTTLQQYEQRTVRLPLGLFSHSDQAMHWQTSLPDQRSAI
                     GWAGRMADLLHGINDNSNISMNISLGGSNVFQSGMNTFHYTISQDGSFGLRDYGAPSP
                     SAMVRTEAIDGLLGLTYQNLFERTFAARMRGAIDAHLEFSAAIESLPPLQTVFSDTGL
                     SQRLRMVAQTIQARELLGFRRQTFFVEQGGWDHHDEVLVNQENQLPVVSAALSEFQSA
                     MYELGVADEVTVFTASDFGRTLTSNGRGSDHAWGGNMLVLGGAVRGGDFYGSYPELYQ
                     GNSLDTGRGRLIPTTSCDEYFAELALWFGVPRTDLELVLPNIRRFYDPASAAPPLGYL
                     VGPSMGRNVTPTDRRDRRLELITPRNSRRGAARS"
BASE COUNT          887 a         2185 c         2195 g         1026 t
ORIGIN      
        1 agcgcgctgg ccctcccgtg gctcgtcaag tttctctggg cgccgctcgt cgatcgctac
       61 gggtcggtcc gcttcggcca ttaccgctcg tggatcgtcc cgctccagct gctgacggtg
      121 ctcgcggtcg tcgcgttgag ccgggtcgac ctcggccgcg gcctgaccgg gctgctcgtc
      181 gccgccggcg ccttcatgct ctgctcggcg acgcaggaca tcgcgaccga cggactcgcc
      241 gtgcgctcgc tgcgccgcga agagcggggt ctggccaacg gcatccaggt cggcggctac
      301 tatctcgggc agatcctcgg cggcggtttg atcctgatcg tcttcagccg cttcggctgg
      361 agcgccgccg tgctgtcgat ggcgctgttc ctcgcgctgc cgctctggcc gaccacccgc
      421 tttcgcgagc ccggccatcc gccgggccgg acgcccggcg tgcgcgtcga ctggaaggcg
      481 ctcggtcggt tcgccacgcg ctcgggtgcg ggcgtctgga tcctgatcct gctgctctac
      541 cgctcgggcg aggcgatggc gctgacgatg gtcaacccga tgctcgtcga cctcggcctg
      601 acgctcgacg agatcggcct gttgctcggt ctcgcgggct cgttggcctc gttcgccggc
      661 gccgtcggtg gcgggctgtg gatcgcgcgg gtcggtcggc ggcgatcgct ggtcgtgttc
      721 ggcctgctgc aggcggtggc gctcggcggc tacctgctgc ccgcctccgg tttcacctgg
      781 ctccccgcga tctatgccgt gacgatgacc gccgcctgct gctcggggat ggcgaccgcc
      841 gcgctctaca ccaacatgat ggaccgctgc gacccggcga ccggcgccac cgacttcacg
      901 ctgcagcagt cgttgtgcgc ggtcgggccg ctggtcgcgg cgtcgctcag cggcttctcc
      961 gcggcgtcgc tcggctacgc gatgcacttc ggcatgacgg tcgcggtcgc gctgatcgcg
     1021 gtcggtgtcg tcgccgccgg gctgaccgaa gcgagcggcg ctccgcgccc ggcggtcggc
     1081 gccgtgtcga ccgcggccga cgcctgattg cgtcgcgggc cgcccggcct accttagaat
     1141 ggagcgtgat cgcgaggcga gccgatgtgt tccattctct gtgtcttcga catccagcac
     1201 gacgccgcgg cgctccgcgc ccgcgcgctc gagctgtcgc ggcgcctgcg tcatcgcggc
     1261 ccggactgga ccggcatctt ctcgagcgag cgtgcgatcc tggcccacga gcggctggcg
     1321 atcgtcgacg tcgagcacgg ggcgcaaccg ctcgagagcg gggacggccg ctacgtgctc
     1381 gccgtcaacg gcgagatcta caaccacgcc gcgctcgagc gagcgctcgc ccggccgtac
     1441 gagttccgga ccaagtccga ctgcgaggtg atcctgccgc tgttcgccca gcgtgacacc
     1501 ggcttcctcg acgagctgaa cggcatcttc gccttcgcga tctacgatcg cgacgaggac
     1561 cgctacctgc tggcgcgcga tccgatcggc gtgatgccgt tgtactacgg ccacgacgag
     1621 cacggtcggg tgtgggtcgc ctccgagatg aaggggctga tcgacgtctg tcggtcgttc
     1681 gccgagttcc cgccgggaca ctacctcgac agccgcgccg gcgagccggt gccctactac
     1741 cggccggtct ggcgcgacta cgccgcggtg cgcggccagg cgcccgatct cgcccggctg
     1801 cgcacgtcgc tcgaggccgc cgtcgagcgg cagctgatgt gcgacgtgcc gtacggcgtg
     1861 ctgctgtcgg gcggcctcga ctcgtcgatc ctctccgccg tctgccacaa gttctacaag
     1921 acgcgcgtcg agaacggcgg gcgcagcgag gcctggtggc cgcagctcca ctcgttcgcc
     1981 atcggcctcg tcggctcgcc cgacctggcc gccgcgcgca aggccgccgc ggcgatcggc
     2041 acgatccacc acgagcacac ctacacgatc cagcaggggc tcgacgccgt ctccgacgtg
     2101 atctaccacc tcgagaccta cgacgtcacg accgtccgcg cagcgacgcc gatgttcctg
     2161 ctggcgcgcc ggatcaaggc gatggggatc aagatggtgc tgaccggcga gggatcggac
     2221 gagatcttcg gcggctacct ctacttccac aaagcgcccg acgccgagga gttccacgcc
     2281 gagacgctgc gcaagctcga ccagctccac ctgttcgact gcctgcgggc caacaaggcg
     2341 ctggcggcgt ggggcgtcga ggggcgcgtg ccgtttctcg accgcgagtt cctcgacgtc
     2401 gcgatgggct tcgacgcctg ccacaagatg tgcggcggcg atcggatcga aaagcacgtg
     2461 ctgcgcgagg cgttcgccgg gtacctgccg caggagatcc tctggcggca gaaggagcag
     2521 ttctcggacg gcgtcggcta cgcctggatc gacacgctca agcgcgtggc cgaggagcag
     2581 atcggcgacc agcagttcgc ccgggccgcg cgccgctttc cctcgcacgt gccggcaacc
     2641 aaggaggcct acctgtaccg cgcgatcttc gaggggcact tccccggcga ggccgcggcg
     2701 gccaccgtgc cgcacggcat gagcatcgcc tgcagcacgc cgaccgcgat ccgctgggac
     2761 gagatgttcc gcacgcgcgc cgacccgtcg ggacgcgcga tgaagggtgt ccaccgcgac
     2821 gccttttgac tgcgacccgc gcccggtccg tcgtgacttc gcctcacgat cgacgtatat
     2881 tacgcagccg agcaggttcg gtttagaatg gtggtaccgg gtgaggtgcc cgatgcgcga
     2941 ttcgaccaaa cagacgccgg tctgggccag aacgctcgac acgatcgcgg cccgcatcga
     3001 gcaggcgccc gcggcgcgca agcgcgacct cgaccccggc gatcgcgtga tcgtcgcgac
     3061 gcgcaactcg gtctactgcc tgcaggcgct cggcggcgat ctgttcgagg tgtcgggcgg
     3121 ctggttcgag cggaaccccg actcgcgact gatcacggtc aacggctgca cctacggcgg
     3181 gctggcgatc cacaccgaca tcgtcgccgc gcgcgggctg ttcctcgagt tcggcaacaa
     3241 cgtctcgacg acgcgcatcc agcgcgtgct ggtcgtgcgc ggcaaccggc agtcggccgc
     3301 ggccggccac tcgaacctgc ccaactgaac ggggtgcgca cccccgcttc gctgccggcg
     3361 atcgtcgttt gggcgatcgc cctgcccgcg gtggcggggg cgggcgcggc cggcgatccg
     3421 ttggtcgtcg cgcccgaggc ctacgagctg cgcgccgaga acggatcgtt ccgcgtgctc
     3481 gagctgagcc tggcgccggg cgagcgggac gagttccacg gccacccgcc cggcgtctat
     3541 tacgtgatcg agggcggccg cgtgcggatc cacacgccgg cgggcgcgcc gcgcgacgtc
     3601 gagctcccga cgggccacgt cggcttccaa tccgccgtcg tctcgcaccg catcgagaac
     3661 atcggcccga cgacgattcg cgtgctgctg atcgaggacc ggcgcccggc taccaggcga
     3721 agatcgccgc ggcgatcagc cgatcgaact cgatcacgcg ccgcgacagg ccggtgctct
     3781 cgcccggcgc cggcgacaac gcgacctctt cgccgaagcg ccaggccagg tcgaaggtca
     3841 tgttgaggaa gacgccctcg acgccgatcg cgtactcggt cgtcgtacgt gtgctgccgt
     3901 cggtcgacgt gctggcgtcg aacacgtcct tcatctcgcg gtcgagaaag tcggcgcggg
     3961 tgctggcgat cagccgcagc ttgtcggtgc cgagcggctg gcggatgccg agcttgaggc
     4021 cgagggcggt cagcgtgtcg tcgatcgact cgctcgacgt gttgacgccg tcaccgatct
     4081 cggtcgagcc gtcgagcgtc gacagcgtca gctcggcgcc ggtgaagacc tgggtcgacg
     4141 ggcccgccag gttgatcgag ccgaacgagg cgacgatcga agtctcggtg accgcgtcct
     4201 gacccagtag ggtgacgagc gggccgccct cgtcgaacgc caggtcgctg ttgtcgagcg
     4261 agctttcgat ctggcccagc gtcaagcccg cctgccactc gccgcggagc gcctggaagc
     4321 gccggttgta ggccccgccg aggacgatcg tcgtgtcggt gatgtcgtag ttgaggatgt
     4381 ccgtgcgggc gacgatcgcg ccggtgtcgt cgagcgactc ggcgaactcg ttctgctcgg
     4441 tcgagccgtt gccgtagatc agctgcgcct gccagctcga ccgctcgtcg aggaagtggc
     4501 gcacgccgac gtcgaagccg aagcccgagc gggtcacctc gtcggtgccg ttgaacccgc
     4561 cgatccccag ctcgaacgag ctggccgtct cctcgtcgtc gcggccgctg aggtcgatgc
     4621 cgccgccgat ggtgaagctc tcgttcaaca cgtaggccgc ggcgaagcgc aggtcgtagc
     4681 ccgtcagcga gttcgtcacc cggatcgagt cgaccggatt ggtgccggtg ttctcgttgt
     4741 cgtcggcgaa cagcgaggcc gagatacccc agttgaacgc cgagccgggc tggcggatgt
     4801 aggcgcccga gaagacgtcc gagccctgct ggacgttggt gttgccgttg ttgccgacca
     4861 gcgcgttgac gtagtggcgg tggtcgcctt cctggtcggg ccacgggctg aggttgccgt
     4921 ggcgatcgat gttgacgaac ggcaggttga acggacgccg cccgagctcg tcgtacggat
     4981 cccagaacag gtcgtgcgtc aactgattga tcaccgatcg ccggaaggtc aatggcgaat
     5041 cgcgctcgtc ggcgatctcg cgcgtgacgg ccaggaccga acccggaagc aacagggcga
     5101 tcgcgcacag cacgaccgcg acgcgtctga ttctgctcac ctcaagacct cccctgtcgt
     5161 aaagacccct tcggtcgttc gtcgctcagg acctcgcggc gccccggcga ctgttgcgcg
     5221 gtgtgatcag ctcgagccgc cgatcgcgcc ggtcggtcgg cgtgacgttg cggcccatcg
     5281 acggtccgac gagataaccc aaaggcggcg cggccgaggc cgggtcgtag aaccggcgga
     5341 tgttcggcag caccagctcg agatcggtcc gtggcacgcc gaaccacagc gccagctcgg
     5401 cgaagtactc gtcgcacgag gtcgtcggga tcagccggcc gcgaccggtg tcgagcgagt
     5461 tgccctggta cagctcggga tagctgccgt agaagtcgcc gccgcgcacc gcgccgccca
     5521 gcaccagcat gttgccgccc caggcgtggt ccgagccgcg gccgttcgag gtcagcgtgc
     5581 ggccgaagtc ggaggcggtg aagaccgtca cctcgtcggc cacgccgagc tcgtacatcg
     5641 cgctctggaa ctcgctgagt gcggcgctga cgaccggcag ctggttctcc tggttgacca
     5701 gcacctcgtc gtggtggtcc cagccgccct gctcgacgaa gaacgtctgc cggcggaagc
     5761 cgagcagctc gcgcgcctgg atcgtctgcg ccaccatgcg gagccgttgc gacaagccgg
     5821 tgtcggagaa gaccgtctgc agcggcggca gcgactcgat cgccgcactg aactcgaggt
     5881 gcgcgtcgat cgcgccgcgc atgcgggcgg cgaaggtccg ctcgaacagg ttctggtagg
     5941 tcagcccgag caggccgtcg atcgcctcgg tccgcaccat cgcgctcggc gacggcgcgc
     6001 cgtagtcgcg cagcccgaag ctgccgtcct gactgatcgt gtagtggaag gtgttcatcc
     6061 cggattggaa gacgttgctg ccgccgagcg agatgttcat cgagatgttc gagttgtcgt
     6121 tgatgccgtg gagcaggtcg gccatccgcc cggcccaacc gatcgcgctg cgctgatcgg
     6181 gcagcgacgt ctgccagtgc atcgcctggt ccgagtgcga gaacagaccg agcggcaggc
     6241 ggacggtgcg ctgctcgtac tgctgcagcg tcgtcggctc gaccagcgtg ccg
//
LOCUS       DASPGN010000070         3617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_481574, whole genome shotgun sequence.
ACCESSION   DASPGN010000070 DASPGN010000000
VERSION     DASPGN010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481574"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="VD788_02040"
     CDS             complement(<1..406)
                     /locus_tag="VD788_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75071.1"
                     /translation="MPARARLAVFAAPFFAETTVRFVRAAAGLPGVRLAVLSQDPLDR
                     MPSDLRRAIAAHQRIPDALDATAIADAVAELASVAGRPVDRLVGALEQLQVPLAQVRE
                     RLGIDGMGVEAALNFRDKSRMKDVLRAAGVPCA"
     gene            502..1956
                     /locus_tag="VD788_02045"
     CDS             502..1956
                     /locus_tag="VD788_02045"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HXV75072.1"
                     /translation="MRICFPASEVAPFSKTGGLADVSGALPRELARAGHEVRVFTPLY
                     DAIDRGRHRIVPFDPVRRVAVALGRRTYFFSLATTESPTPGHRVFLVDCPELFGRGEI
                     YSTAPDEPQRFALFSRAVFESCQRMRWAPEVLHCHDWHTALIPLLRKTVYAWDDAIFG
                     GCRTMLTIHNLGYQGLFGADVVDAVGLGDQRALVDQDDLSHGRFNFLRTGLLHADLVS
                     TVSPTYAREIQTAAYGMGLAPLLRARGDRLVGILNGVDHDEWDPESDPFLPHHFSANN
                     LAGKRLIRRDLLERLRLSDVADAPLVAIVSRLVPQKGFDIAFPVLPPLLAGRRIGLVV
                     LGQGDAHYHDFFAGLERRFSGRVVFHQGYSTELAHVIEAAADIFLMPSQYEPCGLNQL
                     FSLRYGTIPLVRRTGGLADSVEHFDGRANSGTGFVFEHYTSDGLAWALEQALATFGDK
                     PRWRRLVVNAMSVDFSWRHQAQRYVETYERLTGG"
     gene            1987..3207
                     /locus_tag="VD788_02050"
     CDS             1987..3207
                     /locus_tag="VD788_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75073.1"
                     /translation="MHVLFVEPGFPYNQREFVRALYMAGAAVTGIGERPLGALDEELK
                     NWMVGYEQIPSVVHEPPLLDAVTRCQRRGWVDRLEATIEAHVMAAAKVREARGIPGTS
                     VRTTFLCRDKPAMKQALREAGIPCAASTGTASIDEALEFARATGYPIIVKPRDAAGAS
                     GTHRVGDDGELRSVLERCGVGAGASVALEEFIEGHEGFYDTVCRDGRVRHEFVTHYFP
                     NVLEAMRTRWISPQFVATNRIGADGYTEVKQLGQRVIEALGIDTSATHMEWFFGPNGL
                     KFSEIGCRPPGVGAWDLYCAANEMDLYLEWALAVVGRPPARAASRRYSAGIIALRPDQ
                     DGVIAGYSGLERMHQLYRDQLIDSHFPPAGTPTAPVEAGYMANAWVRMRHPDYDELRG
                     MLDDVGRTVKVHAR"
     gene            3409..>3617
                     /locus_tag="VD788_02055"
     CDS             3409..>3617
                     /locus_tag="VD788_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HXV75074.1"
                     /translation="MGRRSKLDELLERRILVLDGAMGTMIQRQKLDESDFRGELFRDH
                     ARDLKGCNDVLSLTRPELIERIHRD"
BASE COUNT          544 a         1229 c         1239 g          605 t
ORIGIN      
        1 gggcgcacgg cacgccggcc gctcggagca cgtccttcat cctggatttg tcgcggaaat
       61 tgagcgcggc ctcgaccccc atcccgtcga tgcccaggcg ctcgcgtacc tgggccagcg
      121 gcacctgcag ctgctcgagc gcgccgacca accggtcgac cggccgcccg gccaccgacg
      181 ccagttcggc gaccgcgtcc gcgatcgccg tggcatcgag cgcgtcgggt atgcgttggt
      241 gcgccgcgat cgcgcggcga aggtccgacg gcatacggtc gagcggatct tggctcagca
      301 cggccaagcg aacaccgggc agcccggcgg cggctcgaac gaaacgcacg gtggtctccg
      361 cgaagaacgg cgccgcgaat acggccagtc gtgcgcgggc tggcatcggt cttccctgtg
      421 ttagcatgtc gcgtccgatt ctagcctgcg cgcggcgcac ccggaccgct ttccacgcca
      481 tactggagat ccgcccgacc gatgagaatc tgttttccgg cgtccgaggt ggcgccgttt
      541 tcgaagacgg ggggtctggc cgacgtttcc ggcgcgctgc cgcgcgagct ggcgcgcgcc
      601 ggtcacgagg tccgcgtctt caccccgttg tacgacgcaa tcgaccgtgg ccggcaccgg
      661 atcgtcccgt tcgacccggt gcgccgggtc gcggtcgcgc tcggacggcg gacctacttc
      721 ttctcgctgg cgacgaccga gagcccgacg ccgggacatc gcgtgttcct ggtggactgt
      781 cccgagctgt tcggccgcgg cgagatctac tcgacggcac ccgacgagcc ccagcgcttc
      841 gcgctgtttt cgcgcgcggt gttcgagagc tgccagcgca tgcgctgggc tcccgaggtc
      901 ctgcactgcc acgactggca caccgccctg atcccgttgc tgcgcaagac ggtctatgct
      961 tgggacgatg cgatcttcgg cggctgtcgc acgatgctga cgatccacaa cctgggctat
     1021 cagggtctgt tcggcgccga cgtggtggac gccgtggggc tcggcgacca gcgcgcgctc
     1081 gtcgaccaag acgacctgtc ccacggccgg ttcaacttcc tgcgtaccgg tctgctccac
     1141 gcggatctgg tcagcacggt cagtccgacc tacgcccgcg agatccagac cgcggcctac
     1201 gggatggggc tggcgccgct gctacgggcg cggggcgacc ggctggtcgg catcctgaac
     1261 ggggtcgacc acgacgagtg ggacccggaa tccgacccgt tcctgcccca tcacttctcg
     1321 gcgaacaacc tcgccggcaa gcggttgatc cggcgcgacc tgctcgagcg gctgcgcctg
     1381 tccgatgtcg ccgacgctcc gctcgtcgcg atcgtctcgc gcctcgtccc gcagaagggc
     1441 ttcgacatag ccttccccgt tctgccgccg ttgctcgccg gacgacggat cggactggtg
     1501 gtgttggggc agggtgatgc tcactaccac gatttcttcg ccgggctgga acggcgcttt
     1561 tccggccgcg tggtcttcca tcagggatac agcaccgagc tggcgcacgt catcgaagcc
     1621 gcggcagaca tcttcttgat gccctcacaa tacgagccat gcggcttgaa ccagctgttc
     1681 agcttgcgct acgggacgat tccgctggtt cgtcgcaccg gcgggctggc cgacagcgtc
     1741 gagcacttcg acgggcgagc gaacagcgga accggtttcg ttttcgagca ctacacgagc
     1801 gacggtctgg cgtgggcgct cgagcaagcg ctcgcgacct tcggtgacaa gccgcgctgg
     1861 cgacgcctgg tggtcaacgc gatgtcggtc gacttctcct ggcgtcacca ggcccagcgt
     1921 tacgtcgaga cgtacgagcg gttgaccggc ggctgaccgg gtcaccgcgg ggtgggaggt
     1981 cgtgtgatgc acgtactgtt cgtcgagccg ggctttccct acaaccagcg ggagttcgtc
     2041 cgcgcgctct acatggcggg agccgccgtc accggtatcg gtgagcgccc gctcggcgcg
     2101 ctcgacgaag aactgaagaa ctggatggtc ggctacgagc agattcccag cgtggtccac
     2161 gaaccgccgc tgctcgacgc ggtcacgcgc tgccagcgtc gtggatgggt cgaccggctg
     2221 gaggcgacga tcgaggcgca cgtgatggcg gcggccaagg tgcgcgaggc gcgcggcatt
     2281 cccggaacca gcgtgcgcac gacctttctc tgccgtgaca agccggcgat gaagcaggcg
     2341 ctgcgcgagg cgggcatccc ctgcgccgcg tcgacgggca ccgcctcgat cgacgaggcg
     2401 ttggaattcg cccgcgcgac gggctacccg atcatcgtca agccgcggga tgccgccggc
     2461 gcttcgggga cgcatcgcgt cggcgacgac ggcgagctgc ggagcgtgct cgagcgatgc
     2521 ggcgtcggcg ccggtgcgtc ggtcgcgctg gaggagttca tcgagggtca cgaggggttc
     2581 tacgacaccg tctgtcgcga tggccgcgtg cggcacgagt tcgtcacgca ctatttcccg
     2641 aacgtcctcg aagcgatgcg tacgcgctgg atctcgccgc agttcgttgc gaccaatcgc
     2701 atcggagccg acggctacac cgaggtcaag caactcggcc agcgggtaat cgaagcgctc
     2761 gggatcgaca cgtcggcgac ccacatggaa tggttcttcg gtcccaacgg gctgaaattc
     2821 tccgagatcg gctgccggcc gccgggcgtc ggtgcctggg atctgtattg cgcggccaac
     2881 gagatggacc tgtacctcga gtgggccctg gccgtcgtcg gccgcccgcc tgcccgcgcg
     2941 gcctcccgac gctactcggc gggcatcatc gccctgcgtc cggaccagga cggcgtcatc
     3001 gccggttact ccggcttgga gcgcatgcac cagctctacc gcgaccagtt gatcgacagt
     3061 cactttccgc cggcgggcac gccgaccgca ccggtcgagg ccggttacat ggccaacgcc
     3121 tgggtccgca tgcgccaccc cgactacgac gagctgcgcg ggatgctcga cgacgtggga
     3181 cgcacggtca aggtgcacgc gcgatgaacg accgcggggt cgagccttgc cccgtctgcg
     3241 gcgcgccgcg ggtgcgggtc aagtgtaagc tgaccgcgcg aagtgccgtg ccgtgatcgc
     3301 caaccgcaac ggcgattgac tcggcgggac ggtctgtcat aacccttgcc cgcgcgatcc
     3361 gccggtcgga tgctctgcta agctggttcg tgccggagga gtcggatcgt gggtcgacgt
     3421 agcaaactcg acgagctgct cgagcgacgc attctcgtgc tcgacggcgc gatgggcacg
     3481 atgatccagc ggcagaagct ggacgaatcg gactttcgcg gtgagctctt ccgcgaccac
     3541 gcgcgcgatc tgaagggctg caacgacgtg ctgtcgctga cccgtcccga gctgatcgag
     3601 cggatccacc gcgacta
//
LOCUS       DASPGN010000071         6892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_364283, whole genome shotgun sequence.
ACCESSION   DASPGN010000071 DASPGN010000000
VERSION     DASPGN010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364283"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1070)
                     /locus_tag="VD788_02060"
     CDS             complement(45..1070)
                     /locus_tag="VD788_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXV75075.1"
                     /translation="MPPRAPVGAARLRLVLGSLLLVLAGVGLAAASLGAGRLSLLALW
                     QSVSADERIARTILLDVRLPRVALAGLLGGALTVAGLVFQALLRNPLADPYILGVAGG
                     ASIGGVLALVLGPATWLYGAGLPAAAFCGALAALLLIERIATVGGRLTVYTVLLTGAI
                     FNAFSAAVIYLLQSMASLDQLHSIVFYLMGRIPSLGWHSIGAIAVPIVAAVLLLAARA
                     RDYNVLTLGEEGAAYLGVDVERLKRQTFVVGSLLTALTVSVAGMIGFVGLIVPHLLRL
                     LFGPDHRLLIPAGFLGGASFLILADLVSRTLISPDELPVGVVTALVGGPFFLYLLRRR
                     GRGRVFD"
     gene            complement(1070..1960)
                     /locus_tag="VD788_02065"
     CDS             complement(1070..1960)
                     /locus_tag="VD788_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HXV75076.1"
                     /translation="MRGRTLASTIAAAGMLTACGQPEVVRQADGTPQRVIVLAPAAAE
                     MLDALGLAGRVVAVGEYGPWPESMADRPSVGGYMSPNVELAVALRADRVLTTASEAAT
                     EIHAGLEQVGIEVEPLDTSTYDGVFSALERVGQLFEREEQAGRLATELRARLDRIARA
                     ASGAPARRVLFVVGRDPLYVAGPGSHLDQMIRLVGGVNVAADAGAPYRRLSLEAVLER
                     RPEIIIDTSDNGAGAKRGRVAGTWGEWDFLPAVRHDRVYQVDPGRLVIPGLRLAEMTE
                     LVGKLVQPEIFGEAEPRELD"
     gene            complement(1967..2674)
                     /locus_tag="VD788_02070"
     CDS             complement(1967..2674)
                     /locus_tag="VD788_02070"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HXV75077.1"
                     /translation="MLGRRPFDRLTDGLGSRYAALLVVVLVSIAIHPLVGVHRWVGWL
                     RDLTYVALVVAVLQAVARHVRVYYASLALGGCAAVVSILTDSFGFDELYPYGAVLRLS
                     LSAVVVVVILVDVFGRDEIDTDAVFGAACAYLLAGLGCGALYALVDWFLPGSFDAPMQ
                     PQAVTDRFGPETVESRLVYFSLVTMTTIGYGDVTPVSPPARTLAALQGLLAQLYVAII
                     VARLVGLELVTRSRRNR"
     gene            complement(2679..3749)
                     /locus_tag="VD788_02075"
     CDS             complement(2679..3749)
                     /locus_tag="VD788_02075"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXV75078.1"
                     /translation="MEHAERRGRDGTTRWLILAASFVVIIAGIKAAASLLLPFIVALF
                     LAMVSLPLLTWLHARRVPSGLAVLITVTVVAAVVFSLGALIGGSISSFTDAAPRYRLR
                     LEQMLVELLDWLTARGLTIPQDYTREIVRPGAMMDLAATTLRGVASVLSNLLLVFLTV
                     VFIYLEAAGFPAKLQAAFGRAGGVERYERIRREINRYLSFKTLISLVTGVLIGSAMAV
                     IGVDFPVVWGTLAFLLNYIPNLGSIIAAIPPVLLAAVQLGWGEAFAAALVFLVVNLTL
                     GSLIEPYLMGRRLGLSTLVIFVSLVFWGWVWGPVGMLLSVPITMIIKITLENSTDLKW
                     IAVLLDAAPPPEEPIAEEAERT"
     gene            complement(3777..5201)
                     /locus_tag="VD788_02080"
     CDS             complement(3777..5201)
                     /locus_tag="VD788_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HXV75079.1"
                     /translation="MTGLLPDGLRPSYLHLRAAPDPLALFRGLYDGQHHRFLYESRGE
                     RGGRGRYSFLGGRPLAIFRSRDRRIEIERDGRKLVLDADPLSVLKEMLTGGVECLPIA
                     TFPGGAVGYLGYDLVHRLVPVPGGERDDLGLPDAQFLFPGEVVIVDHLDEVVHVVVYG
                     TADERDRCNEIGALARRSAAAVRVERDAAATADPDDGPEAFRTSPAAGGFRESVRRAK
                     DYIRQGEIYQVVLSQRFDFPLRASPLAIYAALRRENPSPYLYLLDFDGMQLAGSSPEV
                     LVKLTGRRAVTRPLAGTRPRGATPERDASFERELRQDPKERAEHVMLVDLARNDLGRV
                     CRVGSVTVDEFLEVERYAKVMHLVSNVEGRLRDDRDAFDLLAATFPAGTVSGAPKVRA
                     MQIIRELERFRRGPYAGAIGYFGFHGDMDMCIAIRTLLAHRGRGYLQAGAGIVADSDP
                     DAEQAEIWNKARGTFRALARAGTG"
     gene            complement(5198..6229)
                     /gene="aroF"
                     /locus_tag="VD788_02085"
     CDS             complement(5198..6229)
                     /gene="aroF"
                     /locus_tag="VD788_02085"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXV75080.1"
                     /translation="MLVVMKQGVSEAEVERVCRAVRELGLDPHPIPGRSRTAVGITGN
                     LEPVDPARLAALPGVVELISVTKPYKLTGREMKPEDTVVRVGDVEIGGAELVVIAGPC
                     SVESDEQTLTTARAVRAGGARMLRGGAFKPRTSPYSFQGLGQQGLHILEAARRETRLP
                     VVSEAVDIESFDAVERSVDVIQIGSRNMQNYSLLRRAGRSPKPILLKRWMSGTLKEFL
                     MAAEYVLAAGNANVVLCERGIRAFSDYSRYTLDISIVPELKRLTHLPVIVDPSHAAGK
                     RDLVIPLARAAVAAGADGLMVEVHPQPEAALSDGAQALTPALFEKLMREIDRLAPALD
                     RSTSKPAGA"
     gene            complement(6217..>6892)
                     /gene="trpA"
                     /locus_tag="VD788_02090"
     CDS             complement(6217..>6892)
                     /gene="trpA"
                     /locus_tag="VD788_02090"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HXV75081.1"
                     /translation="HRIFGWGADVVEVGVPFSDPVADGPVIQRAAHDALAGGTRLRRI
                     LDALESDRPPGPVVLMSYLNPLLALGRQLAAERIGRAGVSGLIVPDLPADEARPWLES
                     TRLHGIELIPLVAPTSDERRIASVLACSDGLLYYVSVTGTTGVRRELDAGLPDALDRL
                     RRSTGRPVAAGFGISTPEHVQRLRRHCDGVVVGSRIVEAIRRDEDLAELIGRLKEATR
                     GGRCWS"
BASE COUNT         1197 a         2448 c         2362 g          885 t
ORIGIN      
        1 gaccgtagga aaacgacagc ccgcgggccg acaccatcgg cgtctcagtc gaagacacga
       61 ccgcgccccc gtcgtctgag caggtagagg aagaacgggc cgccgaccaa tgcggtgacc
      121 acgccgaccg gaagctcgtc cggtgagatc aacgtacgcg acaccagatc ggcgaggatc
      181 aggaagctcg cgccgccgag gaatcctgcg ggtatgagca gccgatggtc ggggccgaac
      241 aacaggcgca ataggtgagg tacgatcagg ccgacgaagc cgatcatccc cgcgaccgac
      301 accgtcaacg cggtcagcag cgagccgacg acgaaggtct gtcgcttcag ccgctcgacg
      361 tccacgccga ggtaggccgc gccttcctcg ccgagcgtca ggacgttgta gtcgcgggcg
      421 cgggccgcga gcagcagtac cgccgcgacg atcgggacgg cgatcgcgcc gatcgagtgc
      481 cagccgagcg acgggatgcg acccatcagg tagaagacga tgctgtgcag ctgatcgagc
      541 gacgccatgc tctgcaacag atagatcacg gccgcgctga acgcgttgaa gatcgcgccg
      601 gtcaacagga cggtgtagac cgtcaaccgg ccgccgacgg tcgcgatgcg ctcgatcagc
      661 agcaacgcgg ccagtgcccc gcagaaggcc gcggccggca gacccgcacc gtagagccac
      721 gtcgccggcc cgagcaccag cgccagcacg ccgccgatgc tcgctccgcc ggcgacgccc
      781 aggatgtagg gatcggccag cgggttacgc agcaacgcct ggaacacgag gccggccacg
      841 gtcagcgcgc cgcccaagag cccggccagc gcgacccgcg gcagccggac gtcgagcagg
      901 atggtgcggg cgatccgctc gtcggccgag accgactgcc acagggccag cagcgacagg
      961 cgccccgcac cgaggctggc ggcggccaac cccacgccgg ccagcacgag caacagcgag
     1021 ccgagcacca ggcgtagccg cgcggcaccc accggcgccc gaggcggcat cagtcgagct
     1081 cccgaggctc tgcctcgccg aagatctcgg gttggaccag cttgccgacg agctccgtca
     1141 tctcggccag cctgagaccg ggaatgacca ggcggcccgg gtcgacctgg tacacccggt
     1201 cgtggcggac ggccggaagg aagtcccact caccccacgt tccggccacg cgtccgcgct
     1261 tcgcgcccgc gccgttgtcc gaggtatcga tgatgatctc gggtcgccgc tcgaggaccg
     1321 cctcgagcga tagccggcga tagggcgcgc cggcgtcggc ggccacgttc accccaccga
     1381 ccagccggat catctggtcg aggtgcgagc cggggccggc gacgtagagc gggtcgcgac
     1441 cgacgacgaa cagcacccgt ctcgcgggtg ctccgctcgc ggcgcgcgcg atccggtcga
     1501 gtcgcgcgcg aagctcggtc gccagccggc cggcctgctc ctcccgctcg aacagttgcc
     1561 cgacgcgctc gagcgcggag aagaccccgt cgtacgtgct ggtgtcgagc ggctcgacct
     1621 cgatcccgac ctgctcgagc ccggcgtgaa tctcggtcgc cgcctcgctc gccgtggtca
     1681 gaacccgatc ggcccgcagg gcgacggcga gctcgacgtt gggcgacatg tagccgccga
     1741 cgctcggtcg gtcggccatc gactcgggcc acggtccgta ctccccgacg gcgacgacgc
     1801 gtcccgccag accgagggcg tcgagcatct cggccgcggc cggcgcgagc acgatcacgc
     1861 gttgcggcgt cccgtccgcc tggcgcacga cctcgggctg gccgcacgcc gtcagcatcc
     1921 cggcggcggc gatcgtcgat gcgagcgttc gacctctcat cgcgcctcac cgattccggc
     1981 ggctacgcgt caccagttcc aggccgacca accgcgcgac gatgatcgcg acgtacagct
     2041 gcgcgagcag gccctggagc gcggccagcg tgcgggcggg cggcgagacg ggcgtgacgt
     2101 cgccatagcc gatcgtcgtc atcgtgacga ggctgaagta gaccaaccgc gactcgaccg
     2161 tttccggccc gaaccgatcg gtcaccgcct ggggttgcat cggagcgtcg aacgagccgg
     2221 gcaggaacca gtcgaccagc gcatagagcg cgccgcagcc cagcccggcc aacaggtagg
     2281 cgcaggcggc gccgaagacg gcgtcggtgt cgatctcgtc gcgaccgaat acgtcgacca
     2341 ggatgacgac gacgacgacc gcgctcagcg agaggcgcag cacggcgccg tacggataga
     2401 gttcgtcgaa gccgaacgag tcggtcagga tgctgaccac cgccgcgcag ccgccgagcg
     2461 ccaggctcgc gtaatagacc cgtacgtgtc gtgcgacggc ctgcagcacc gcgacgacga
     2521 gcgcgacgta ggtcagatcg cgcaaccagc cgacccagcg atggaccccg accagcgggt
     2581 ggatcgcgat cgacaccaac accacgacga gcagtgcggc ataacggctg cccagcccgt
     2641 cggtcaggcg gtcgaacggg cgtcgtccga gcatcccctc acgtcctctc cgcttcttcg
     2701 gcgatcggtt cctcgggggg cggagcggcg tcgagcagta cggcgatcca cttcaggtcg
     2761 gtggagttct cgagcgtgat cttgatgatc atcgtgatcg ggaccgacag cagcatcccg
     2821 accggccccc agacccaccc ccagaatacc agcgagacga agatcaccaa cgtcgacaga
     2881 cccaggcgcc gtcccatcaa gtagggctcg atcaacgaac cgagcgtcag gttgacgacg
     2941 aggaagacga gggccgcggc gaacgcctcg ccccaaccga gctgaaccgc ggcgagcagt
     3001 acggggggga tggcggcgat gatcgaaccc aggttgggaa tgtagttcag caggaaggcg
     3061 agcgtgcccc acaccaccgg gaagtcgacg ccgatgaccg ccatcgccga accgatcaag
     3121 acgccggtca cgaggctgat cagcgtcttg aagctgaggt agcggttgat ctcccggcgg
     3181 atccgctcgt agcgctcgac gccacccgcg cggccgaacg ccgcctgcag cttggccggg
     3241 aacccggctg cttcgaggta gatgaacacg accgtcagga acacgagcag caggttcgac
     3301 agcacggagg ccacgccgcg cagtgtcgtc gcggccaggt ccatcatcgc gccggggcgc
     3361 acgatctcgc gcgtatagtc ctgcgggatc gtcaggcccc gcgccgtcag ccaatcgagc
     3421 agctcgacca gcatctgctc gagccgcagc cggtaacgcg gcgcggcgtc ggtgaaactc
     3481 gaaatcgatc cgccgatcag cgcaccgagg ctgaacacga ccgcggcgac gacggtgacc
     3541 gtgatcaaga ccgcgagccc cgacggtacg cgcctggcgt gcagccaggt cagcagcggc
     3601 agactcacca tcgccagaaa gagcgcgacg atgaacggga gcagcaggct ggcggccgct
     3661 ttgatcccgg cgatgatgac gacgaacgat gccgcgagga tcagccaacg ggtcgtcccg
     3721 tcccgtcccc gtcgctccgc gtgctccatc ggcacactat atacgaggac cgccgatcat
     3781 ccggtgccgg cccgcgccag cgcgcggaag gtaccgcggg ccttgttcca gatctccgcc
     3841 tgttcggcgt cgggatcgga gtcggcgacg atgcctgccc cggcctgcag gtagccccgc
     3901 ccgcgatggg ccaacagcgt acggatcgcg atgcacatgt ccatgtcacc atggaagccg
     3961 aagtaaccga tcgcaccggc gtacggcccg cggcgaaagc gctcgagctc gcggatgatc
     4021 tgcatcgcac ggaccttggg cgccccggaa accgtcccgg cggggaaggt cgcggccagc
     4081 aggtcgaacg cgtcgcggtc gtcgcgcaac cgaccctcga cgttgctgac gaggtgcatc
     4141 accttggcat accgctcgac ctcgaggaac tcgtcgaccg tgacactgcc gacgcggcag
     4201 actcgaccga gatcgttgcg cgccagatcg accagcatca cgtgctcggc ccgctccttc
     4261 gggtcctgcc gaagctcccg ctcgaaggac gcgtcccgct cgggcgttgc cccgcgagga
     4321 cgcgtgccgg ccagcggccg cgtgacggca cggcgtccgg tcagcttgac gagcacctcg
     4381 ggggacgagc cggcgagctg catcccgtcg aaatcgagca ggtacaggta gggtgacgga
     4441 ttctcccggc gcagcgcggc gtagatcgcg agcggcgacg cgcgcaacgg gaagtcgaag
     4501 cgctgcgaga gcacgacctg atagatctcg ccctgccgga tgtagtcctt ggcgcgccgg
     4561 accgactcgc gaaagccgcc ggccgcgggc gacgtgcgga acgcctcggg gccgtcgtcg
     4621 ggatccgccg tcgccgccgc gtcgcgctcc acccggacgg cggccgcgga tcgtcgtgcc
     4681 agcgccccga tctcgttgca gcggtcgcgt tcgtccgccg tgccgtaaac cacgacgtgg
     4741 acgacctcgt cgagatggtc gacgatgacg acctcgcccg gaaagaggaa ctgcgcgtcg
     4801 ggaagtccga gatcgtcccg ctcgccgccg gggaccggga cgagccgatg aacgaggtcg
     4861 tagccgaggt agccgaccgc gccgccgggg aaggtggcga tgggcaggca ctcgacgccg
     4921 ccggtgagca tctccttcag caccgacaac gggtcggcgt cgaggacgag cttccgcccg
     4981 tcacgctcga tctcgatccg tcggtcgcgg ctgcggaaga tcgccagcgg acgaccgccg
     5041 aggaacgagt agcgaccccg tccgccgcgc tcaccgcgag actcgtagag gaatcggtga
     5101 tgctggccgt cgtacagacc gcggaacagc gcgagcgggt cgggtgccgc gcgcaggtgc
     5161 aggtaggacg gtcgcaatcc gtccggcagc agaccggtca cgcgcccgcg ggtttcgacg
     5221 tcgagcgatc gagcgccggt gccaggcgat cgatctcgcg catcagcttc tcgaacaggg
     5281 cgggggtgag cgcctgcgcc ccgtcggaca gggcggcctc gggctgcggg tggacctcga
     5341 ccatcagccc gtcggcgccg gccgcgaccg ccgcgcgggc cagcgggatg accaggtcgc
     5401 gcttgccggc ggcgtggctc gggtcgacga tcacgggaag gtgggtcaac cgcttcaatt
     5461 ccggcacgat gctgatgtcg agcgtgtacc tcgagtagtc gctgaacgcg cggatgcccc
     5521 gttcgcacag gaccacgttc gcgttgcccg cggccagcac gtactcggcg gccatcagga
     5581 actccttgag cgtgccggac atccagcgct tgagcaggat cggtttcggc gagcggccgg
     5641 cccgtcgcag cagcgagtaa ttctgcatgt tgcgtgagcc gatctggatc acgtcgaccg
     5701 agcgctcgac ggcgtcgaag ctctcgatgt cgacggcctc ggagacgacg ggaagtcgag
     5761 tctcgcgccg tgcggcctcg aggatgtgga ggccctgctg gcccagaccc tggaacgaat
     5821 agggcgacgt gcgcggcttg aacgcaccgc cgcggagcat ccgcgcccca ccggcgcgaa
     5881 ccgcccgcgc cgtcgtcaac gtctgctcgt cggactcgac cgagcacgga ccggcgatga
     5941 ccaccagttc cgctccgccg atctcgacgt cgccgacacg cacgaccgtg tcctcgggct
     6001 tcatctctcg cccggtgagc ttgtacggct tggtcaccga gatcagttcg accacaccgg
     6061 gcagtgccgc cagccgggcc ggatcgaccg gctccaagtt gccggtgatc ccgaccgcgg
     6121 tccgcgaccg acccgggatc ggatgcgggt cgagccccag ctcgcgcacc gcgcgacaaa
     6181 cccgctccac ctcggcttcg ctcacgccct gcttcatcac gaccaacatc ggccacccct
     6241 cgtcgcttcc ttcaatcggc cgatcaactc ggccaggtcc tcgtcgcggc ggatcgcctc
     6301 gacgatacgg ctgccgacga cgacgccgtc acaatgtcgt cgcagccgct gaacgtgttc
     6361 cggtgtcgag atgccgaacc cggccgcgac cggccgaccg gtcgagcgtc gcaggcggtc
     6421 gagcgcgtcg ggcaggccgg cgtccaactc gcgccgtacg cccgtcgtgc cggtgacgct
     6481 gacgtagtac agcagtccgt cggagcaggc gagcacgctc gcgatgcgcc gttcgtcgga
     6541 ggtcggtgcg acgagcggga tcagctcgat cccgtggagg cgcgtcgact cgagccacgg
     6601 cctggcctcg tcggccggca ggtccggaac gatcagcccc gacaccccgg cgcgcccgat
     6661 gcgctccgcc gccagctggc ggccaagtgc cagcagcggg ttgagatagg acatcagcac
     6721 caccggaccc ggtggacggt ccgactcgag cgcatcgagg atccgacgca gccgcgtgcc
     6781 gccggccagc gcgtcgtgcg ccgcgcgctg gatcaccggt ccgtcggcca cggggtcgct
     6841 gaacgggaca ccgacctcga ccacgtccgc cccccagccg aagatccggt gc
//
LOCUS       DASPGN010000072         3337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1301191, whole genome shotgun sequence.
ACCESSION   DASPGN010000072 DASPGN010000000
VERSION     DASPGN010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301191"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VD788_02095"
     CDS             complement(<1..364)
                     /locus_tag="VD788_02095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXV75082.1"
                     /translation="MGVSPHPDTNLGRHGQASFEDMSPWVRPPANPRPALREEVRADV
                     VVIGAGYTGLSTALSLAAAGADVVVLERDFAGAGASGRNAGHLTPTIGKDLPTLLKLF
                     GRERAARLVRFADDAVAHT"
     gene            452..1789
                     /gene="leuC"
                     /locus_tag="VD788_02100"
     CDS             452..1789
                     /gene="leuC"
                     /locus_tag="VD788_02100"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXV75083.1"
                     /translation="MDAPRNIYEKIWDRHLVHHEPGQDAILYIDRHLVHEVTSPQAFE
                     GLRLAGRRVRRPELTFATMDHNIPTTGRTHSIEDPLSRRQVEALEANCREFGVTLFGL
                     EDRRSGIVHIIGPELGITQPGQTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTL
                     LQRKAKTMEVRIDGSLPTGVTPKDVILAVIGKLGTAGGTGYVIEYTGDVVRSLSMEGR
                     MTICNMSIEAGARAGLISPDRTTFEYLRGREYVPKDEPYDRVCARWATWASDPGCRYD
                     KTVTIDAADIANADARQATAKALEYMGLAEGIAINSVEINRVFIGSCTNARIEDLREA
                     AKVVKGYRLSSKIDEALIVPGSQQVKAQAEKEGLDRIFVDAGFEWRDSGCSMCLGMNP
                     DQLRPGDRCASTSNRNFEGRQGKGGRTHLVSPAMAAAAAVEGRFTDVRSWEFH"
     gene            1789..2382
                     /gene="leuD"
                     /locus_tag="VD788_02105"
     CDS             1789..2382
                     /gene="leuD"
                     /locus_tag="VD788_02105"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXV75084.1"
                     /translation="MERFTRIEGIVAPLEATNVDTDQIIPKQFLKRIERTGYEDFLFF
                     DWRYLDDGKTPDPRFEPNADRYRGASILLTKDNFGCGSSREHAPWALYDYGFRAILAP
                     SFADIFYSNCFNNGMLPIMLANAEIDRLFGEVRAQVGYRLTIDLPSQTITKPGGATIP
                     FEIDPFLKERLLNGWDQIGLTLRREQEIDDYERRHGI"
     gene            2554..>3337
                     /locus_tag="VD788_02110"
     CDS             2554..>3337
                     /locus_tag="VD788_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75085.1"
                     /translation="MVGAASLLAGALAHPTDSFGQVCGDPVALTAGASSRPAVITAPD
                     ALFVLRIAVALGSCDLCVCDVNNSGGITAGDALLVLGAAVGQPVVLACPSCVPVSTTS
                     TTLCFELEEDNLNFGVTEDSSGVPDSAVATEAAQSEHPADLFSSPGSGAHAPGAAGDN
                     SLTLEDVDLGLAPGDELDAISFGPVAVDPFEVHFSLRDSPRVMGVEGTDVRIQSETEV
                     VPGDVYTSTGHPSPVRGSNAISLDEIALGLQPHDLTSDPTDDL"
BASE COUNT          564 a         1140 c         1085 g          548 t
ORIGIN      
        1 cggtgtgggc gaccgcgtcg tcggcaaagc gcaccaagcg ggctgcgcgt tcgcgaccga
       61 acagcttgag cagtgtcggc aaatccttgc cgatcgtggg cgtcagatga ccggcgttgc
      121 ggccgctcgc gcccgctccc gcgaaatcgc gctcgagaac gacgacgtcg gcgcccgcgg
      181 cggccagcga caacgccgtc gacagcccgg tgtaacccgc accgatgacg acaacgtccg
      241 cgcgcacctc ctcgcgtagg gcgggacgcg gattcgccgg gggtcgcacc cacgggctca
      301 tgtcctcgaa ggacgcctgc ccatgccggc cgaggttggt gtcgggatgc ggtgacacgc
      361 ccattcaggc tgccgcttac gaccggatga caccagaaga ccgggcaggc cgattgattt
      421 gtaccctggc cgcgtgttct ttaaagatcg gatggacgcc ccacgcaaca tctacgagaa
      481 gatctgggac cggcacctgg tgcatcacga gccgggccag gacgcgattc tctacatcga
      541 ccgtcacctc gtccacgagg tcacgtcgcc gcaggcgttc gagggcctgc gcctggccgg
      601 ccgccgtgta cgccggcccg agctcacgtt cgcgaccatg gatcacaaca ttccgaccac
      661 cgggcgcacc cattcgatcg aggatccgct gtctcggcgc caggtcgaag cactcgaagc
      721 gaactgccgg gagttcggcg tgacgctgtt cggcttggag gataggcgca gcggtatcgt
      781 ccacatcatc ggcccggagc tcggcatcac gcagcccgga cagacgatcg tctgcggcga
      841 ctcgcacacc tccacgcacg gtgctttcgg cgcgctggcc ttcggcatcg gcacgagcga
      901 ggtcgagcac gtcttggcga cccagacgct gctgcagcgc aaggccaaga cgatggaggt
      961 ccgcatcgat ggctcgctgc cgacgggggt gacgccgaag gacgtcatcc tggccgtgat
     1021 cggcaagctc ggcacggcgg gcggcaccgg ctacgtgatc gagtacaccg gcgacgtcgt
     1081 gcgctccctc tcgatggaag gtcgcatgac gatctgcaac atgtcgatcg aggccggcgc
     1141 acgtgccggc ttgatctcgc ccgaccggac cacgtttgag tacctgcgcg ggcgcgagta
     1201 cgtgcccaag gatgagccgt acgatcgggt gtgcgcgcgc tgggctacct gggcgtccga
     1261 ccccggctgc cgctacgaca agaccgtcac gatcgatgcc gccgacatcg cgaacgccga
     1321 cgcgcgacag gcgacggcca aggcgctcga gtacatgggg ctggccgagg ggattgcgat
     1381 caactcggtc gagatcaacc gcgtgttcat cggctcgtgc acgaacgcgc gcatcgagga
     1441 cctgcgcgaa gccgccaagg tggtgaaggg ctaccgattg agctcgaaga tcgatgaggc
     1501 gctgatcgtg ccgggctctc agcaggtcaa agcccaggcg gaaaaagagg ggctcgatcg
     1561 gatcttcgtc gacgccggct tcgagtggcg cgactcgggc tgctcgatgt gcctcggcat
     1621 gaatcccgac cagctgcgtc cgggcgaccg ctgcgcttcg acgtccaacc gcaacttcga
     1681 aggccgccaa ggcaagggcg gacgcaccca cctggtgagc ccggcgatgg ccgccgccgc
     1741 cgctgtcgaa ggacgcttca ccgacgtcag gagctgggag tttcactgat ggagcgtttc
     1801 accaggatcg aggggatcgt cgccccgctg gaggcgacca acgtcgatac cgaccagatc
     1861 atcccgaagc agttcctcaa gcgcatcgag cgcaccggct acgaggactt cctgttcttc
     1921 gactggcgct atctcgacga cggcaagacg cccgatccgc gcttcgagcc gaacgccgac
     1981 cgctaccgag gcgcgtcgat tctcttgacc aaggacaact tcggctgcgg ctcctcgcgc
     2041 gagcacgccc cctgggcgct ttacgactac ggcttccgtg cgatcctcgc accctcgttc
     2101 gccgacatct tctactcgaa ctgcttcaac aacggcatgc tgccgatcat gctggcgaac
     2161 gcagaaatcg accgcctgtt cggcgaggta cgcgcacagg tcggctaccg gctgaccatc
     2221 gacctgccga gccagaccat aacgaagccg ggcggcgcga cgattccgtt cgagatcgac
     2281 ccgttcctca aggagcgtct gctcaacggc tgggaccaga tcggcctgac gctgcgccgc
     2341 gagcaagaga tcgacgacta cgagcgccgc cacggcatct gaatactccg agtcgcctcc
     2401 gatcgggcgg gcgacgcttg tgcggcgtgg actagcgcga gcccggccgc tgcgctatca
     2461 gacccgtcga gaaaaacgcg cgtggaccct ttcgtcggtc gttctcgctt tcgctctcgc
     2521 ccggccgcct gctcgcggtc gagcttttcg ctcttggtcg gtgccgcctc cttgcttgca
     2581 ggcgcgctgg cgcatcccac ggattctttt ggccaggttt gtggcgatcc ggtcgccttg
     2641 acggcggggg cgtcctcgag accggccgtc atcaccgcac ccgatgcgtt gttcgtgctg
     2701 cgcatcgcag tcgccctcgg cagctgtgac ttgtgcgtgt gcgacgtcaa caacagcggt
     2761 ggcatcacgg ccggtgacgc cctgctcgtg ctcggcgcgg cggtcggaca gcccgtggtg
     2821 ctcgcttgcc cctcctgcgt tccggtatcc acgacgtcga ccaccctgtg cttcgagctc
     2881 gaggaggaca acctgaactt cggcgtgact gaggacagca gcggcgtgcc cgactcggcg
     2941 gtggcgaccg aagctgccca atccgagcac cccgccgatc tgttctccag cccgggcagc
     3001 ggtgctcacg caccgggcgc tgccggtgac aactcgctga ccctcgagga cgtcgatctc
     3061 ggccttgctc ctggcgacga gctcgacgcg atctcgtttg gcccggtcgc ggtcgacccc
     3121 ttcgaggtgc acttctcctt gcgagattcg ccccgtgtga tgggagtaga ggggaccgac
     3181 gtccgcatcc agagcgagac cgaggtggtg ccgggcgacg tctataccag caccggccac
     3241 cccagcccgg tgcgtggcag caacgcgatc tcgctcgacg agatcgccct cgggctgcag
     3301 ccgcacgacc tgacgagcga tccaaccgac gatctgg
//
LOCUS       DASPGN010000073         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1418075, whole genome shotgun sequence.
ACCESSION   DASPGN010000073 DASPGN010000000
VERSION     DASPGN010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1418075"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3140)
                     /locus_tag="VD788_02115"
     CDS             complement(<1..>3140)
                     /locus_tag="VD788_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75086.1"
                     /translation="GDVDVCATLGNPDQGMPVRLSDTIALGMDEIEARISADGTRVVY
                     RSDRDASGAFRLYSVPITGGTPVELNPALVAGGGVEQFEISADGTLVVYLADQEVDDR
                     VELYRVPIDGGAATKLNTALGATGDVVEFALAGSRVVYRADPAADTLFDLFSVPIDGG
                     AVVPLSNAAGSGADVVAFKVSPDTSQVAYFRVERVFSTAVQLFSTTVASAANVVLDTF
                     APSGDFVGFEISPDSTRVVYLADADVDGVDELFRATIGVAGSAVRLNPVLSGGRDVTD
                     FGISGTSGRVVYVADQASDEVFELFSVPLGGGAADRLNDPLPAGGDVFAFRSTDGGSR
                     VVYLADQLANDVYGLFAKDVTGGTRDTLSATGVSVIDFAQPRGARVLYRADAGDGVPR
                     LFSAALDGGGTPVELSGSLVAGGGARLYGSTADGLTAVFDADALVVNRYEVFAVPAAG
                     GQRARLSGPMPPGGSQVGLLRVSPDGARVVYLADQETDQLFDLYAALIRTDRDGDGVW
                     DPCDVCPGIADATQADADGDGAGDSCDNCDLAYNPGQEDGDGDGVGDACDPCPEDFGG
                     FLDSDGDGVGDACETCPLDPRIVGGTPLVVDGPGLGTAVVAGSGNFAYTPDGERIVYA
                     LQNVAATWEVRAAPRSGGPATLLFTSPLEPERLAVSPDGGRVAIRWRDDPGLPASLAS
                     VALGGGPATVLHDPLPPGVGVTDFEFTADGARVVYIAAQDTHGVPELYSVPVAGGSPV
                     RLHPGLPAGRQVVDFALSPSSDRVAYVADTEGAYELYSVAVTGGAVVKLNRPLVAGGN
                     VDGSPVVSGDGQRVVYRADQDTDEVFELYTVPLLGGVATRLSAPLLAGSIDPYGVAWI
                     LLGDGQTVFYLAEADTPGVRELYAVPATGGGSTRVNTPLVPGREIVSVSERSGDTALY
                     VADQDTDDVYELYRASIAGGGGTRISAPQVPGGDVSDAAISPDGTRVVYLADQDTNDL
                     SELYGVALAGGPAVKLNDLTSGAGIVGFTFAAGDTVVFLESAAAQALYSSRSDGSGAT
                     RLHDPFPATAEFVAAA"
BASE COUNT          479 a         1141 c         1102 g          418 t
ORIGIN      
        1 atcgccgccg ccacgaactc ggcggtcgcg gggaacggat cgtgaagccg cgtcgcccca
       61 ctgccgtcgg agcgcgagga ataaagcgcc tgtgcagcgg ccgactcgag gaacacgacc
      121 gtgtcgccgg cggcgaaggt gaagccgacg attccggcgc cggaggtgag atcgttgagc
      181 ttgaccgccg gaccgccggc gagcgcgacc ccgtacagct cggacaggtc gttcgtgtcc
      241 tggtcggcca ggtagacgac acgcgtgccg tcggggctga tcgccgcgtc cgagacgtca
      301 cccccgggca cctgcggggc actgatccgc gtcccgccgc caccggcgat cgatgcgcgg
      361 tacagctcgt agacgtcgtc cgtgtcctgg tcggcgacgt acagcgccgt gtcccccgac
      421 cgttcgctga cggagacgat ctcgcgtccc gggacgagcg gcgtgttgac ccgcgtcgat
      481 cctccacccg tggccgggac ggcgtacagc tcgcgcactc cgggggtgtc cgcttcggcg
      541 aggtagaaca cggtctgccc gtcaccgagc agaatccatg cgactccgta cggatcgata
      601 ctgcccgcga gcagcggcgc gctgagccga gtggccaccc cgccgagcag cgggaccgtg
      661 tacagctcga agacctcgtc cgtgtcctgg tcggcgcggt agacgacgcg ctgcccgtct
      721 ccactgacga ccggcgaccc gtcgacgttc ccgccggcga cgagcgggcg gttgagcttc
      781 acgaccgccc ccccggtgac ggcgaccgag tacagctcgt acgcaccctc ggtgtcggcg
      841 acgtacgcca cgcggtcgct cgacggcgag agcgcgaagt cgaccacctg gcgacccgcc
      901 ggaagcccgg ggtgtaaccg caccgggctt ccgccggcga ccggcacgga gtagagctcg
      961 ggtaccccgt gcgtgtcctg ggccgcgatg tacacgacgc gcgctccgtc ggcagtgaac
     1021 tcgaagtcgg tcacgccgac tcccggcggc agcgggtcgt ggagcacggt cgccgggccc
     1081 ccgccgagcg ccacgctcgc gagcgacgcc gggaggcccg gatcgtcgcg ccagcggatc
     1141 gccacacggc cgccgtccgg gctgaccgcg agccgttccg gctcgagcgg ggaggtgaag
     1201 agcagggtgg caggcccgcc ggacctcggg gccgcccgaa cttcccacgt cgcagcgacg
     1261 ttctgcagcg cgtagacgat acgctcgccg tccggtgtgt aggcgaagtt tcccgagccg
     1321 gcgacgacag ccgttcccag cccggggccg tccacgacga gtggcgtgcc gccgacgatc
     1381 cgcggatcga gcgggcaggt ctcgcaggcg tcgcccacgc cgtccccgtc gctgtcgagg
     1441 aacccgccga aatcctcggg gcacggatcg caggcgtccc cgaccccgtc gccgtcgccg
     1501 tcttcctgcc ctgggttgta cgcgaggtcg cagttgtcgc aagagtcccc cgcgccgtca
     1561 ccgtcggcgt cggcctgggt cgcgtcggcg atgcccggac agacgtcgca cgggtcccaa
     1621 acaccgtccc cgtcgcggtc ggtgcggatc aacgccgcgt agaggtcgaa gagctggtcg
     1681 gtctcctgat ccgccaggta gaccacgcgc gcgccgtccg gcgacacccg gagcagaccg
     1741 acctgcgagc caccgggcgg catcggtccg ctgagccgag cacgttgacc accggccgcc
     1801 gggacggcga agacctcgta gcgattgacg accagcgcat cggcatcgaa caccgcggtg
     1861 agaccgtcgg cggtcgaccc gtagagccgc gctcctcctc cggcgacgag ggagcccgag
     1921 agctcgacgg gcgtgccgcc gccgtcgagc gcggcgctga acagacgggg tacaccgtct
     1981 ccggcgtcgg cgcggtacag cacacgagct ccgcgcggct gggcgaagtc gatcacgctc
     2041 accccggtcg cgctcagcgt gtcgcgcgta ccgccggtca cgtccttggc gaagagcccg
     2101 tagacgtcgt tcgccagctg gtcggcgagg tagaccaccc ggctgccgcc gtcggtcgat
     2161 cggaaggcga acacgtcgcc accggccggg agcgggtcgt tgaggcggtc cgcggcgcct
     2221 ccgccgaggg gtaccgagaa caactcgaac acctcgtccg acgcctgatc cgcgacgtag
     2281 accacgcgcc cgctcgtgcc gctgatgccg aagtcggtga cgtcgcggcc gccggagagc
     2341 accgggttga gccggaccgc cgatcccgcg acgccgatcg tggcccggaa cagctcgtcg
     2401 accccgtcga cgtcggcgtc ggccaggtag acgactcggg tcgaatcggg gctgatctcg
     2461 aagccgacga aatcgcctga tggagcgaag gtgtcgagca cgacgttcgc ggcgctcgcg
     2521 accgtcgtgc tgaagagctg aaccgcggta gagaagaccc gctcgacgcg gaagtaggcc
     2581 acctggctcg tgtccgggct gaccttgaac gccaccacgt cggcaccgga tccggcggcg
     2641 ttgctcagcg gcacgaccgc cccgccgtcg atcggcacgc tgaacaggtc gaagagcgta
     2701 tccgccgccg gatccgcccg gtagaccacg cggctgccgg cgagcgcgaa ctcgaccacg
     2761 tcacccgtgg cgccgagcgc cgtgttgagc ttcgtcgccg ccccgccgtc gatcggcacg
     2821 cggtaaagct ccacccggtc gtcgacctcc tgatcggcga ggtagaccac gagtgtaccg
     2881 tcggcgctga tctcgaactg ctccacgccg cccccggcga cgagcgcggg attgagctcg
     2941 accggcgtgc cgccggtgat cgggacgctg tagagccgaa acgccccgct ggcgtcacga
     3001 tcggagcggt agacgacccg agtcccgtcc gcgctgatcc gcgcctcgat ctcgtccatt
     3061 ccgagggcga tggtgtccga caggcggacc ggcattccct ggtcgggatt gcccagcgtc
     3121 gcgcagacgt cgacgtcgcc
//
LOCUS       DASPGN010000074         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_481896, whole genome shotgun sequence.
ACCESSION   DASPGN010000074 DASPGN010000000
VERSION     DASPGN010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481896"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1652
                     /gene="pyk"
                     /locus_tag="VD788_02120"
     CDS             243..1652
                     /gene="pyk"
                     /locus_tag="VD788_02120"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXV75087.1"
                     /translation="MARKAKIAATLGPASDATQAMAQLVRSGLDVARLNFSHGSPDEH
                     AARVERLRLVARAAERIVGVMADLQGPRFRVGTLPGGSLTLADDAVVELVAGRKRAPH
                     GALPVSHAALARDVKQRQAILIDDGRIRLQVERVRDDRVRCRVVRGGKVAERKGINLP
                     GTDLSVPTITPKDRRDLETAVEIGADWLAVSFVRSARDVRQARRLLAKAGSTMPVMAK
                     IERPEAIDRLDEILDAADGVLVARGDLGVELAPEDVPILQKQIIEAANAAGKPVVTAT
                     QMLDSMRHSAQPTRAEASDVANAVLDGSGCLLLTAETAVGEYPVEAVRMMARIIERAE
                     NAGRTRVEDGPHANLSIAVATAHAGCRAAAEVGARYLVAFTSSGSTAFHVSRFRPITP
                     LVACTTRFEIARRMTLLWGVTPVVVERRANFEGLVETVDDNLRRAGLAEAGDVVVLIA
                     GAPVGMPGTTNAIKVHRLG"
     gene            1658..>2090
                     /locus_tag="VD788_02125"
     CDS             1658..>2090
                     /locus_tag="VD788_02125"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXV75088.1"
                     /translation="MRATVSVNGELSDGGSAVVSVFDQGFLYGEGVYETIRTYGGVPF
                     LFDRHTARLRRSASLLALDVPFDDDELRARVDRTVDAHRPVAEAYVRILLTRGVGELS
                     YDPRTCRGPTLVIIVKDFVAPSARAYSEGIALVLAGVRRNHP"
BASE COUNT          303 a          724 c          769 g          294 t
ORIGIN      
        1 gcccgccgtc gtgtccgccc cgccggtggc gccaccgatc gacaacgaca ggcaacacgc
       61 cacggccgcg acccgcatcg gccacctcct cgctcggtcc ggcgagcagc gtagcccgtg
      121 acggcccgca ccgctgtaaa atcgaccgtg acatcggtaa agcgatgtaa acgaccgacg
      181 tcggctcgcg gtcgccgcgc ggcatcgaaa cgcgctgcga cccccgggga gttccgctcc
      241 gtatggcgag aaaggccaag atcgccgcga ctctcggtcc ggcgagcgac gccacgcagg
      301 cgatggcgca gctggtccgg tcggggctcg acgtcgcgcg gctcaacttc tcgcacggct
      361 cgcccgacga gcatgcggcg cgcgtcgagc gcttgcgcct cgtggcgcgc gccgccgagc
      421 ggatcgtcgg cgtaatggcc gatctgcagg gcccgcgctt tcgcgtcggg actctgccgg
      481 gcggatcgct gacgctggcc gacgacgcgg tggtcgagct cgtcgccggg cggaagcgcg
      541 cgccgcacgg cgccctgccg gtctcgcacg ccgcgctcgc acgcgacgtg aagcagcgac
      601 aggcgatcct gatcgacgac ggccggatac ggcttcaggt cgagcgggtg cgtgacgacc
      661 gcgtgcgctg ccgcgtggtg cgcggcggca aggtcgccga gcgcaagggg atcaacctgc
      721 cgggcaccga tctttccgtg ccgacgatca cgccgaagga tcgccgcgac ctcgagacgg
      781 cggtcgagat cggagcggac tggctcgccg tctcgttcgt gcggagcgcg cgcgacgtcc
      841 gtcaggcgcg ccgcctgctg gcgaaagccg ggtcgacgat gccggtgatg gcgaagatcg
      901 agcggccgga ggcgatcgac cggttggacg agatcctcga cgcggccgac ggagtgctcg
      961 tggcgcgggg cgacctcggc gtcgagctcg cgcctgagga cgtgccgatc ctgcagaagc
     1021 agatcatcga ggcggcgaac gcggccggca aacccgtggt gaccgcgacc cagatgctcg
     1081 acagcatgcg tcactccgcg cagccgacac gggccgaggc gtcggacgtc gccaacgccg
     1141 tgctcgacgg cagtggctgt ttgttgttga cggccgagac ggcggtcggc gagtatccgg
     1201 tcgaggccgt gcgcatgatg gcgcgcatca tcgagcgcgc ggagaacgcg ggtcgcacgc
     1261 gcgtcgagga cggcccgcac gcgaacctgt cgatcgcggt cgcgacggcc cacgccggct
     1321 gtcgcgccgc ggccgaggtc ggcgcgcgat acctcgttgc cttcaccagc agcggctcga
     1381 cggcgttcca cgtctcgcgt ttccgcccga tcaccccgct cgtggcctgc acgacccgct
     1441 tcgagatcgc gcgccggatg acgttactct ggggagttac gccggtggtg gtcgagcggc
     1501 gggcgaactt cgagggtctg gtcgagacgg tcgacgacaa cctgcgccgg gccggactgg
     1561 ccgaagcggg cgacgtcgtg gtgctgatcg cgggcgcacc ggtcggcatg ccgggcacga
     1621 ccaacgcgat caaggtccat cggttggggt gagccgaatg cgagcgacgg tgtcggtcaa
     1681 cggggagctg tcggacggcg ggtcggccgt cgtctccgtc ttcgatcagg ggttcctcta
     1741 cggcgaaggc gtctacgaga cgatccgcac ctacggcggg gtgccgttcc tgttcgaccg
     1801 acacacggcg agactgcgcc gctcggcgag tctccttgcg ctcgacgtgc cgttcgacga
     1861 cgacgagctc cgtgcgcggg tcgaccggac ggtcgatgcg caccgcccgg tcgcggaagc
     1921 ctacgtccgc atcctgttga cgcgcggcgt cggcgagctg agctacgacc cgcggacctg
     1981 ccgggggccg acgctcgtga tcatcgtcaa ggacttcgtc gccccgtcgg cccgcgccta
     2041 cagcgagggc atcgcgctgg tgctggccgg agtccggcgc aaccatcccg
//
LOCUS       DASPGN010000075         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_599058, whole genome shotgun sequence.
ACCESSION   DASPGN010000075 DASPGN010000000
VERSION     DASPGN010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599058"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VD788_02130"
     CDS             complement(<1..301)
                     /locus_tag="VD788_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HXV75089.1"
                     /translation="MATVRVRFAPSPTGFLHVGGARTALFNWLFARKHGGQFILRIDD
                     TDRQRNVEEALAPILHGLRWCGIDWDEGPEAGGACGPYYQSQKSQRYREAVQTLLD"
     gene            complement(327..418)
                     /locus_tag="VD788_02135"
     tRNA            complement(327..418)
                     /locus_tag="VD788_02135"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(382..384),aa:Ser,seq:gct)
     gene            complement(500..587)
                     /locus_tag="VD788_02140"
     tRNA            complement(500..587)
                     /locus_tag="VD788_02140"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(551..553),aa:Ser,seq:tga)
     gene            complement(574..2295)
                     /locus_tag="VD788_02145"
     CDS             complement(574..2295)
                     /locus_tag="VD788_02145"
                     /inference="COORDINATES: protein motif:HMM:NF011291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HXV75090.1"
                     /translation="MSRRDETTRSPNESRPRDFIRARISEDVAAGKNGGRVHTRFPPE
                     PNGYLHIGHAKAIVLNFGVADEYSGLCNLRFDDTNPTKEEVEYERAIQDDVRWLGYDW
                     GGRLYFASDYFERLYGFAVDLIEAGKAYVDSQSADEIRNNRGTLTEPGRASPYRRRSV
                     ADNLDLFARMRAGEFADGAHVLRAKIDMASPNLNMRDPTLYRIRRTHHHRTGDDWVIY
                     PMYDFTHPISDALEGVTHSLCSLEFEDHRPLYDWVLDNVDLGCRPQQIEFARLNLSYT
                     VLSKRKLLRLVKQGLVSGWDDPRMPTLRGMRRRGYPSSAIRHFVERVGVAKRDSVVDL
                     AQLEFSVREELNRSSPRVMAVLRPMRVVIENYPEGQVEQLEAVNNPENAADGTRLVPF
                     SRVLYLERDDFREDPPRKWFRLAPGREVRLRYAYFITCREVIRNAAGEVEELRCSYDP
                     QTRGGNAPDGRKVRGTLHWVSAAHAVPAEARLYDRLFVKEDPDVDQDFTASLNPDSLE
                     IVRGCRVEPSLVDAEPGDRVQFERLGYFSVDDRRGEQGQLVFNRTVTLRDSWAKIERS
                     DARGRDG"
     gene            complement(2292..3578)
                     /gene="serS"
                     /locus_tag="VD788_02150"
     CDS             complement(2292..3578)
                     /gene="serS"
                     /locus_tag="VD788_02150"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXV75091.1"
                     /translation="MLELSYIREQSDRVRQSLADRGESLSLEPLLELDRVRREMLVEV
                     ERLKQTRNERSREIGSIVKSGGDAEPAKREVRELGERITVVERRLDETSAELDRRLTE
                     LPNVPHDSVPIGRQPQDNPVVRRWGEKPEFAREPLPHWEVGTRLGILDFERAAKISGA
                     RFAVLVGDGALLERALIQFMLDLHTREHGYTEVLPPFMVNSRSLFGTGQLPKFAGDLF
                     RTDPGELWLIPTAEVPLTNLHADEILTADELPRAYCAYTPCFRSEAGSYGKDVRGLIR
                     QHQFDKVELVRFCEPERSYEDLEALTGHAERVLQRLELPYRVVTLCTGDLGFSAAKTY
                     DLEVWLPAQRCYREISSCSNFEAFQARRAKIRYRPAQGDKPRPLHTLNGSGLAVGRTM
                     VAILENCQQADGSVRIPEVLRTYMHGKTRIEPRGTE"
     gene            3682..>3950
                     /locus_tag="VD788_02155"
     CDS             3682..>3950
                     /locus_tag="VD788_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75092.1"
                     /translation="MDKFRITGGQRLRGRVEIGGAKNAALPALAASLLTTERVEIDNL
                     PRVRDVRTMLRVLEQLGATTVADSPSRCAVEISSVGSFEAPYDLV"
BASE COUNT          614 a         1331 c         1338 g          667 t
ORIGIN      
        1 tgtcgagcag ggtctgtacc gcctcccggt agcgctggga cttctgcgac tggtagtacg
       61 gcccgcaggc gccgccggcc tccggcccct cgtcccagtc gatgccgcac cagcgcagcc
      121 cgtgcaggat cggcgccagc gcctcctcga cgttgcgctg ccggtcggtg tcgtcgatgc
      181 gcaggatgaa ctggccgccg tgcttgcgcg cgaacagcca gttgaacagc gccgtgcgtg
      241 cgccgccgac gtggaggaaa ccggtgggcg acggtgcgaa gcgaacgcga acggttgcca
      301 tcggtttaac cctgccgatc acccgctggc ggagagggag ggattcgaac cctcggaagg
      361 gtttgggccc ttcaacggtt tagcaaaccg tcccgttcag ccactccggc acctctcctg
      421 cgggtcgtct ctccgggcaa aggccggatt gtagcgcacg cgccgggcga gtcaaccgag
      481 ggcgcgcgcc gatcgacgat ggcggaggga gcgggattcg aacccgcgag cccgtgaagg
      541 cttccggttt tcaagaccgg tggtatcaac cactcaccca tccctcccgc gtgcgtcgga
      601 cctttcgatc ttggcccagc tgtcgcgcaa cgtgacggtg cgattgaaca ccagctgacc
      661 ctgctcgccg cgccggtcgt cgacggaaaa atagccgagt cgctcgaact gcacgcggtc
      721 cccgggctcg gcgtcgacga gcgacggctc gacacgacag ccgcgcacga tttcgagcga
      781 gtcggggttg agcgaggcgg tgaagtcctg gtcgacgtcc gggtcttcct tgacgaacag
      841 acggtcgtag agtcgcgcct cggccggcac ggcgtgcgcc gccgacaccc agtgcaaggt
      901 gccgcggacc ttgcgcccgt cgggcgcgtt gccgccgcgg gtttgcgggt cgtagctgca
      961 gcgcagctcc tcgacctcgc ccgccgcgtt acggatcacc tcgcggcagg tgatgaagta
     1021 ggcgtagcgc aggcgcacct cacgaccggg agcgagtcga aaccacttgc gcggcggatc
     1081 ctcgcgaaag tcgtcgcgct ctaaatagag cacccgcgag aacggcacga gacgcgtgcc
     1141 gtccgccgcg ttctcggggt tgttgaccgc ctccagctgt tcgacctgtc cctccgggta
     1201 gttctcgatc accacgcgca tcggccgcag cacggccatc acgcgcggcg agctgcgatt
     1261 cagttcctcg cgcacgctga actcgagctg cgccaggtcg accacgctgt cgcgcttcgc
     1321 caccccgacg cgctcgacga agtggcggat cgcggagctg gggtagcctc ggcgccgcat
     1381 cccgcgcaag gtcggcatgc gaggatcgtc ccatccggag accagcccct gcttgaccag
     1441 acgcaatagc ttccgcttgc tcagcaccgt gtagctcagg ttgagccgag cgaactcgat
     1501 ctgctgcgga cggcagccga ggtcgacgtt gtccagcacc cagtcgtaca acgggcggtg
     1561 gtcttcgaac tcgagcgagc agagcgagtg ggtcaccccc tcgagcgcgt cggagatcgg
     1621 gtgggtgaag tcgtacatcg ggtagatcac ccagtcgtcg ccggttcgat ggtggtgcgt
     1681 gcgccggata cggtacaacg tcgggtcgcg catgttgagg ttgggcgacg ccatgtcgat
     1741 tttggcgcgc aacacgtggg cgccgtcggc gaactcgccc gcccgcatgc gcgcgaacag
     1801 gtcgaggttg tccgcaaccg accgtcgccg atacgggctc gcgcggccgg gttcggtcag
     1861 cgtgccgcgg ttgttgcgaa tctcgtcggc gctctggctg tcgacatagg ccttgccggc
     1921 ttcgatcaaa tcgacggcga agccgtacaa ccgctcgaag tagtcggagg cgaagtacag
     1981 ccggccgccc cagtcgtagc ccagccagcg cacgtcgtcc tggatcgccc gctcgtattc
     2041 cacttcttcc ttggtcggat tggtgtcgtc gaaccgaagg ttgcacagac ccgagtactc
     2101 gtcggcgacg ccgaagttca acacgatcgc cttggcgtga ccgatgtgca gataaccgtt
     2161 cggctcgggc ggaaagcgcg tgtgcaccct tccgccgttc ttgccggcgg cgacgtcttc
     2221 gctgatgcgg gcgcggatga agtcgcgcgg tcgcgactcg ttcggggaac gcgtcgtctc
     2281 gtcgcgccgg ctcattccgt acctcgcggc tcgattctcg tcttgccgtg catgtaggtt
     2341 cgcagcacct ccgggatccg caccgacccg tccgcctgct ggcagttctc caggatggcg
     2401 accatcgtgc ggccgacggc cagacccgag ccgttgagcg tgtgcagcgg ccgcggcttg
     2461 tcgccctggg ccggccgata gcggatcttg gcccgccgcg cctggaacgc ctcgaaattg
     2521 gagcaggacg agatctcacg atagcagcgt tgcgcgggca gccacacctc gaggtcgtag
     2581 gtcttggcgg cggagaagcc caggtcgccg gtgcacaggg tgacgacgcg gtagggcagc
     2641 tccagccgct gcagcacccg ctcggcgtgg ccggtcagcg cctcgaggtc ctcgtaggac
     2701 cgttccggct cgcagaatcg caccagctcg accttgtcga actggtgctg gcggatcagg
     2761 ccgcggacat ccttgccgta cgacccggct tcgctgcgga agcacggcgt gtaggcacag
     2821 taggctcgcg gaagctcgtc ggcggtcagg atctcgtcgg cgtggaggtt ggtcagcggg
     2881 acctcggcgg tcgggatcaa ccacagctcg cccggatccg tccggaacag gtcgccggcg
     2941 aacttgggca gttgacccgt accgaacaac gagcgcgaat tgaccatgaa cggcggcagc
     3001 acctcggtgt aaccatgctc gcgcgtgtga aggtcgagca tgaactggat caaggcgcgc
     3061 tcgagcaacg caccgtcgcc gaccagcacg gcgaaacgcg ctccgctgat cttggccgcc
     3121 cgttcgaaat cgaggatgcc gagccgcgtg ccgacctccc agtggggcag cggctcgcgg
     3181 gcgaattcgg gcttctcgcc ccaacgacgc acgacggggt tgtcctgcgg ctgccggccg
     3241 atcggcacgc tgtcgtgcgg gacgttcggc agctcggtca gccgccggtc gagctccgcg
     3301 ctcgtctcgt ccagccgtcg ctcgaccacg gtgatgcgct cgccgagctc gcgcacctcg
     3361 cgtttggccg gctcggcgtc gccgcccgac ttgacgatcg atccgatctc gcgcgaccgc
     3421 tcgttgcgtg tttgcttgag ccgttcgacc tcgaccaaca tctcgcgacg cacgcggtcg
     3481 agctcgagca gcggttcgag cgacaacgac tcgccgcggt cggcgagcga ctgccgaacc
     3541 ctgtcgcttt gctcgcgaat gtagctcagc tccagcacgg tcccacttcc gcacggtccg
     3601 tcgggcgaag gccgcccatt gtagcagcgc tgccggccgg cgggccggtg ctatcatcgt
     3661 ggttctcacc gaggaatcct cgtggacaag ttccggatca ccggtggcca gcggttgcgt
     3721 gggcgggtcg agatcggcgg ggccaagaac gccgcgttgc cggcgctggc cgcctcgctg
     3781 ttgaccaccg agcgcgtcga gatcgacaac ctgccgcgcg tgcgcgacgt gcgcacgatg
     3841 ttacgcgtgc tcgagcagct cggcgcgacg accgtcgccg attcaccgag ccgctgcgcg
     3901 gtcgagatct cgagcgtcgg gtcgttcgag gcaccctacg acctggtcaa
//
LOCUS       DASPGN010000076         6828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_248311, whole genome shotgun sequence.
ACCESSION   DASPGN010000076 DASPGN010000000
VERSION     DASPGN010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248311"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VD788_02160"
     CDS             <1..793
                     /locus_tag="VD788_02160"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HXV75093.1"
                     /translation="VVGPFRAQPSEFAKWTTCLVLAVYLARRVRGNMGLRQLVEMSLI
                     VAAPVGLILLQPDMGTALIFLPIFLTGLLIGGIRWKVLVGVAVAGLLLTPLAWTQLKD
                     YQKERILNVLEPERDPSGVGYQVRQSKIAIGSGGMIGKGLFNGTQGQLNYLPAQHTDF
                     ILSGFSEETGFVGAVGLLGLFYYLLWRGIVAARSAQDRLGTYLCLLMVAWVVGQMAIN
                     VGMILGRLPTIGVPLPFLSYGGSALISAICGMALIVNVRTRRFVN"
     gene            874..3594
                     /locus_tag="VD788_02165"
     CDS             874..3594
                     /locus_tag="VD788_02165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HXV75094.1"
                     /translation="MSVSTRDPRLDDAVFDQILASVQRPSRYIGGEWNQVVKDHGDVD
                     LTFALAFPDVYEIGMSHLGYRILYSLINSRDDTVAERVCCPWPDMADRLREHGRPLVS
                     LESATPLGRFDVVGFSLQYEMTFTNVLEMLDLARIPLRASARREGDPLIVVGGPVVFN
                     VEPLADFVDLVFVGDGETLVPDFLDRLRSLKRDRADRRTVIRECAQIEGIYAPGLYSV
                     ERDPVNRLLIPQPAAGVPYPVMRRIVMDLDDHPFPDRIIVPHGEIVHDRVSVEVMRGC
                     PVGCRFCQAGYIYRPTRERDPNQVRDTVIRSVRATGYDQFSLSSLNTGEYGSIHPVMF
                     DLMDRFEPEKVSISLSSMHASTITPELAQQVRRVRKSGFTIAPEAGTQRMRDVINKNL
                     SEEQILTACRLAFEAGWDHVKLYFMLGLPTETDADVDGMVDLAHEICAIGRRSRGGRT
                     RKPLVTLSASSFIPKPETPFQWLGMERTEELYRKQHRIAERVKRGVRFKHHQCETSFL
                     EGVFSRGDRELGRVLERAFRLGARFDGWEEQFNRRAWDDAFRAEGVDPEFYAYRDLSP
                     EAPLPWHAVHSRVNRKWLALELRRALEQATLSVCGPTDCHGCAPFARDCVKGTVAETT
                     GRQLRSDLPLLATPAAPGPGSPAAVERAPALPRTARGVDAELPSPETRRYRGRFSKTG
                     RLRFLGHLDLTRLLLRALRRAGFELVYTSGFNPKPRVGFGPALAVGIASEAEYIDFET
                     TRPLPADDSARSVNGALPDGLRFDAIVEIDAKARSLGETIAAARYRVESLADVAEARG
                     AIDRWRERLPLEVRRKRKDGKVITFDLEREVLELASLDDGGARITLAMQRDGASIRPE
                     EALEAILAKPSSSSIGLVREELLVNWHGRWVNPILAARAGAAVDVRRAVR"
     gene            3572..4816
                     /locus_tag="VD788_02170"
     CDS             3572..4816
                     /locus_tag="VD788_02170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXV75095.1"
                     /translation="MSVELFASRIDRRLSVAVREDGRLVELRVEPDGDEPRSGRILRA
                     RVTKVLPGLEAAFVDVGGERGAFLHASELPPWEPPDAAPPIERRLHEGQVLTVQVERE
                     AVGEKGARLTCRLALPGPALVLLPGVGSLAVSRRIPQREERARLSRLLEQLPRSAGGW
                     IARTAASGRELPALAAEAASLVERWRDAQQRAGAPNARLPVVLLREPSLLEQTVQDIP
                     ADRLARIVVDDETDRATLLRASPPLDAALARRIETYAGRDSLFANAGIDAEIDRALRP
                     RVWLRSGGYIVIEETEALVSIDVNTAKHTGRTDLEQTVLETNLEAADEVARQLRLRGL
                     GGIIVIDMIDMSEPDSRQRVVERFRRALLADPARTRVVGLSELGLLELTRQSKRPGLA
                     RQLTERCGACNGSGRRRVDRPR"
     gene            complement(4834..5316)
                     /locus_tag="VD788_02175"
     CDS             complement(4834..5316)
                     /locus_tag="VD788_02175"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HXV75096.1"
                     /translation="MSADGRGVRDSLVEMTEIVLPEDTNAKGSIFGGRVLALIDKCAA
                     IVGMRHSRSDVVTVSLDTVTFLNKVRVGHVLMLSGRINAAFGSSMEIEVSVASEDPRT
                     GEHKLTTTALATIVAVDGRGRPKRVAALRLDDDEQRQRAEAAARRRRARLDARVAGGD
                     "
     gene            5455..6738
                     /locus_tag="VD788_02180"
     CDS             5455..6738
                     /locus_tag="VD788_02180"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75097.1"
                     /translation="MNSFKPAIAMLLLLLLATTAAWGQAAVAEARGAVEDEDGNPIVG
                     ATIRFRPQGNPEQVYDGKTNKKGRYYIPGMFNPSEEGMWFVEVEAEGYLPVKMTVETR
                     NAQRVLMGDVRTHQIKAQAKLPEVMIRAFGLGQVDLVMAPEAEVLASAQAAATATATA
                     EAGPAAAAPKVDPWDSALDRVSSGDLAGSLEFFDKAIEEQPDSAERAEAYAKVLYQMK
                     RYDEAEVQALRAKELAPGDVDVLMVLYSIHVGAGDLAAAEEVLVEAGQASPDDPRILR
                     QLAYVAGESGNAQAAIEANLRLTEIDPADADAWLSLADLYASTGQMEQSAAAYEQVIA
                     LDPGGAPQVFFNLGALLMNKPDRTDADTERAVTAFRKAIELKPDYREAHKQLAFALLG
                     TGDRAGAKSQLEAYVALAPNAPDSAQMKALIQSLQ"
BASE COUNT         1053 a         2327 c         2385 g         1063 t
ORIGIN      
        1 ggtcgtcggc ccgtttcggg cgcagccgtc cgagttcgcc aagtggacga cctgtctggt
       61 gctggccgtc tacctcgcgc gccgggtgcg cggcaacatg ggtttacggc agctcgtcga
      121 gatgagtctg atcgtcgcgg ccccggtcgg tctgatcctg ctccagcccg acatgggaac
      181 ggcgctgatc ttcctgccga tcttcctcac cgggttgctg atcggcggca ttcgctggaa
      241 ggtgctcgtc ggtgtcgcgg tcgccgggct gctgctgact ccgctggcgt ggacccaact
      301 gaaggactac cagaaggaac ggatcctaaa cgtcctcgaa ccggagcgcg accccagcgg
      361 agtcggctac caggtgcgcc agtcgaagat cgcgatcggg tcgggcggga tgatcggcaa
      421 ggggctgttc aacggcacgc aaggacagct gaactacctg ccggcccagc acaccgattt
      481 catcctgtcg ggtttctcgg aagagaccgg attcgtcggc gccgtcgggc tgctcggact
      541 gttctactac ttgttgtggc gcggaatcgt cgcggcgcgc tcggctcagg atcgactcgg
      601 cacctacctc tgcttactga tggtcgcctg ggtcgtcggc cagatggcga tcaacgtcgg
      661 catgatcctc ggccggctgc cgacgatcgg cgtcccgctc ccgttcctgt cctacggcgg
      721 gtcggcgctg atctccgcga tctgcggcat ggcgctgatc gtcaacgtca ggacccgccg
      781 cttcgtcaac tgagccggcg gccagactgc ccaccggacc cgccgccggc ctgctaagat
      841 cggtcaggag agcgacaccc ggagggaacg gtcatgtccg tatcgacgcg cgatccgcgt
      901 ctcgacgacg cggtcttcga tcagatcctg gccagcgtgc agcgcccgtc gcgctacatc
      961 ggcggcgagt ggaaccaggt ggtcaaggac cacggcgacg tcgacctgac cttcgccctc
     1021 gcctttcccg acgtctacga gatcggtatg tcccacctcg gctaccgcat cctctactcg
     1081 ttgatcaaca gccgtgacga cacggtcgcc gagcgggtct gctgcccgtg gccggacatg
     1141 gccgatcggt tgcgcgagca cggacgcccg ctggtctcgc tcgagagcgc gaccccgctc
     1201 ggccggttcg acgtcgtcgg cttctcgttg cagtacgaaa tgaccttcac caacgtgctc
     1261 gagatgctcg atctggctcg tatcccgttg cgcgcatcgg cccggcgaga gggcgacccg
     1321 ctgatcgtcg tcggcgggcc ggtggtgttc aacgtcgagc cactggccga cttcgtcgac
     1381 ctggttttcg tcggcgatgg cgagacgctg gttcccgact tcctcgaccg cctgcgcagc
     1441 ctcaagcgcg accgcgccga tcgacggacc gtcatccgcg aatgtgcgca gatcgagggg
     1501 atctacgcgc cggggctcta ttccgtcgag cgcgatccgg tcaaccgcct gttgatcccg
     1561 cagcccgcag ccggtgtgcc gtatccggtg atgcgacgta tcgtgatgga cctcgacgat
     1621 catccgttcc ccgaccgcat catcgtgccg cacggcgaga tcgtgcacga ccgcgtctcg
     1681 gtcgaggtga tgcgcgggtg cccggtcggc tgtcgcttct gccaggccgg atacatctat
     1741 cgtccgacgc gcgagcgcga tccgaaccag gtgcgcgaca cggtgattcg ctcggtgcgc
     1801 gccacgggct acgatcagtt ctcgctgtcg tcgctgaaca ccggcgagta cggttcgatc
     1861 catccggtga tgttcgattt gatggaccgc ttcgagccgg agaaggtttc gatctcgctg
     1921 tcgtcgatgc atgcttcgac gatcacgccg gaactggcgc agcaggtgcg ccgcgtccgc
     1981 aagtccggct tcaccatcgc gccggaagcc ggtacgcagc ggatgcgcga cgtgatcaac
     2041 aagaacctga gcgaggagca gatcctcacc gcgtgccggt tggcgttcga ggccggttgg
     2101 gaccacgtca agctctattt catgctcggg ctcccgaccg agaccgatgc cgacgtcgac
     2161 ggcatggtcg acctggccca cgaaatctgc gcgatcggtc gccgcagccg cggcgggcgg
     2221 acgcgcaagc cgctggtgac gctgtcggcg tcgtcgttca tcccgaagcc cgaaacgccg
     2281 ttccaatggc tcggcatgga gcgtaccgaa gagctctacc gcaagcagca tcgcatcgcc
     2341 gaacgggtca agcgcggtgt gcggttcaag caccaccagt gcgagacgag cttcctcgaa
     2401 ggagtcttct cccgaggcga ccgcgagctg ggacgcgtgc tcgagcgggc gttccgtctc
     2461 ggcgcgcggt tcgacggctg ggaggaacag ttcaatcgcc gggcgtggga tgacgcgttc
     2521 cgcgccgagg gcgtcgatcc cgagttctac gcctatcgcg atctgagccc ggaagcgcct
     2581 ctaccgtggc acgccgtcca ttcgcgcgtc aatcgcaagt ggctcgcgct cgagctgcgc
     2641 cgcgcgctcg agcaggcgac gctcagcgtc tgcggtccga cggactgtca tggctgtgcg
     2701 ccgttcgccc gcgattgcgt caagggcacg gtcgccgaga ccaccggacg ccagctgcgc
     2761 agcgacctgc cgctgctcgc gacgccggcg gcgccgggac cggggagccc cgccgcggtc
     2821 gagcgggcgc cggcgttgcc gcggaccgca cgcggggtcg acgcggagct gccgtcgccc
     2881 gagacgcgcc ggtatcgcgg ccggttctcc aagaccggac gattgcgctt tctcggtcac
     2941 ctcgatctca cgcggttgct gttgcgggcg ttgcgccggg cgggcttcga gctcgtctac
     3001 acctccggtt tcaaccccaa gccgcgcgtc ggtttcggtc cggcgctcgc ggtcggcatc
     3061 gcgtccgagg ccgagtacat cgacttcgaa acgacgcgac cgctgccggc cgacgattcg
     3121 gcgcgatcgg tcaacggggc gctgcccgac ggcctgcgct tcgacgcgat cgtcgagatc
     3181 gacgcgaagg cacggtcact cggcgagacg atcgcggccg cacgctaccg ggtcgaatcg
     3241 ctggccgacg ttgcggaggc ccgcggcgcg atcgaccgtt ggcgcgagcg gttgccgctc
     3301 gaggtgcgcc ggaagcggaa ggacggcaag gtgatcacct tcgatctcga gcgcgaagtg
     3361 ctcgagctcg cgtccctcga cgacggcggc gcgcgcatca ccctcgcgat gcagcgcgac
     3421 ggtgcgtcga tccgacccga agaggcgctc gaggcgatcc tggcgaagcc gtcgtcgagc
     3481 tcgatcgggc tggtgcggga ggagctgctc gtcaactggc acggccgctg ggtcaatccg
     3541 atccttgcgg cgcgggcggg cgcggcggtc gatgtccgtc gagctgttcg ctagccggat
     3601 tgaccggcgg ctgtcggtcg cggtgcgcga ggacggtcgg ctggtcgaac tgagggtcga
     3661 accagacggg gacgagccac gcagcgggag gatcctgcgc gcgcgcgtga ccaaggtgct
     3721 tcccgggctc gaggccgcgt tcgtcgacgt gggcggtgag cgcggggcgt tcctccacgc
     3781 gagcgagctg ccgccgtggg aaccgccgga tgcggcaccg ccgatcgagc gcaggctcca
     3841 cgaggggcag gtgctgacgg tgcaggtcga gcgcgaagcg gtcggcgaga aaggtgcgcg
     3901 gctgacctgc cgtctggcgc tgccggggcc ggcgctggtg ctgttgccgg gcgtcgggtc
     3961 gctcgcggtc tcgcgccgca tcccccagcg cgaggagcgc gcacgattga gtcgactgct
     4021 cgagcagctg ccgcggtccg ccggcggctg gatcgcacgc accgcggcca gcggacgcga
     4081 gctacccgcg ctcgccgccg aggcggcgtc actcgtcgag cgctggcgcg acgcgcagca
     4141 acgggccggc gcgccgaacg cccggctgcc ggtcgtcctc ctgcgggaac cgagcctgct
     4201 cgagcaaacg gtgcaggaca tcccggccga ccgcctcgcg cggatcgtcg tcgacgacga
     4261 gaccgaccgc gcgacgctgc tccgcgcgtc gccaccgctc gacgccgctc tcgcccgacg
     4321 gatcgagacc tacgccggcc gagacagcct gttcgccaac gccggaatcg acgccgagat
     4381 cgaccgcgca ctgcgtccgc gggtctggct gcgctcgggc ggctacatcg tcatcgagga
     4441 gaccgaggcc ctcgtcagca tcgacgtcaa cacggcgaag cacaccggca ggaccgatct
     4501 cgagcagacg gtgctcgaga ccaatctcga ggcggccgac gaggtcgcgc gacagctccg
     4561 gctgcgcggc ttgggcggca tcatcgtgat cgacatgatc gacatgagcg agcccgacag
     4621 ccggcagcgg gtcgtcgagc gttttcgccg agcgttgctc gccgaccctg ctcgtacgcg
     4681 cgtcgtcggt ctcagcgagc tggggctgct cgagctgacc cggcaatcga agcgcccggg
     4741 actggccagg cagctgaccg agcgttgcgg agcctgcaat ggaagcgggc gccgacgcgt
     4801 cgaccgaccg cggtaggcgg cgggcgcgaa ccgtcagtcg ccgccggcga cgcgcgcgtc
     4861 gagtcgtgcc cggcggcgcc gcgccgcggc ttcggcgcgc tggcgctgct cgtcgtcgtc
     4921 gagtcgcagc gcggcgaccc gcttcggccg gccacgcccg tcgacggcaa cgatcgtcgc
     4981 cagcgccgtc gtcgtcagct tgtgctcgcc ggtacgcggg tcctccgagg cgaccgagac
     5041 ctcgatctcc atcgacgagc cgaacgcggc gttgatccgg ccggagagca tcagcacgtg
     5101 tccgacgcgg accttgttga ggaaggtcac cgtgtcgagc gagaccgtca cgacgtcgct
     5161 gcgcgagtgc cgcattccga cgatcgcggc gcacttgtcg atcagcgcca acacccgccc
     5221 gccgaagatc gaccccttgg cgttggtgtc ctcgggcaga acgatctccg tcatctcgac
     5281 gagcgagtcc cggaccccgc gaccatcggc gctcaccacg acctcccgcg cgcgaccccg
     5341 ccgcggcgcg ccgaatcata acaccacgca cgcgacgcgc gttgcccgcg ggccgcaccc
     5401 ggtcctataa tcagaggctg accggaatcg aaggaaaccg tcgagggtat cacgatgaac
     5461 agcttcaaac ccgcgatcgc gatgctcttg ctgctgttgc tcgcgacgac cgcagcgtgg
     5521 gggcaggccg cggtcgccga agcgcgcggt gcggtcgagg acgaggacgg taatccgatc
     5581 gtcggggcca cgatccgctt tcgaccgcag ggcaatcccg agcaggtcta cgacggtaag
     5641 accaacaaga aggggcggta ctacatcccg ggcatgttca acccgtcgga ggaggggatg
     5701 tggttcgtcg aggtcgaggc cgagggctat cttccggtca agatgacggt cgagacccgc
     5761 aacgcgcagc gcgtactgat gggtgacgtc cggacgcacc agatcaaggc gcaggccaag
     5821 cttcccgagg tgatgatccg tgccttcggt ctgggtcagg tcgacttggt gatggcgccc
     5881 gaggccgagg tgctcgcctc ggcgcaagcc gccgcgaccg cgactgcgac cgccgaagcg
     5941 gggccggccg cggccgcacc caaggtcgac ccgtgggaca gcgcgctcga ccgcgtctcg
     6001 agcggcgacc tcgccggctc gctcgagttc ttcgacaagg cgatcgagga gcaacccgac
     6061 agcgccgagc gcgccgaggc ctacgccaag gtgctgtacc agatgaaacg ctacgacgag
     6121 gcggaggtcc aggcccttcg cgcgaaagag ctggcgccgg gtgacgtcga cgtactgatg
     6181 gtgctgtaca gcatccacgt cggtgccggt gacctggccg cggcggagga ggtcctggtc
     6241 gaggccggcc aagcgtcgcc cgacgacccg cgtatcctgc gccagctggc ctacgtcgcc
     6301 ggggagtccg gcaatgcgca agcagcgatc gaggccaacc tccggctgac cgagatcgat
     6361 ccggccgacg cggatgcgtg gctcagcctg gcggacctct acgcctcgac gggacagatg
     6421 gagcagtcgg cggcggccta cgagcaggtg atcgcactcg atccgggcgg ggcgccgcag
     6481 gtgttcttca acctcggagc gctgctgatg aacaagcccg accgcaccga tgccgacacc
     6541 gagcgggcgg tgaccgcgtt ccgcaaggcg atcgagctga agcccgacta tcgtgaggcg
     6601 cacaagcagc tggccttcgc cttgctcggc accggcgatc gcgccggggc gaagtcgcag
     6661 ctcgaggcgt acgtcgccct cgcgccgaac gcacccgact ccgcgcagat gaaggcgctg
     6721 atccagtcgt tgcagtagcg acgatgaacg cggcggagtg gaggctcgac cgtcgatccg
     6781 ggctgttgat cgcggtgtgc tccgcgctcg acgcgatcgc gggcgtcg
//
LOCUS       DASPGN010000077         4646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_599576, whole genome shotgun sequence.
ACCESSION   DASPGN010000077 DASPGN010000000
VERSION     DASPGN010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599576"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1424
                     /locus_tag="VD788_02185"
     CDS             <1..1424
                     /locus_tag="VD788_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75098.1"
                     /translation="MDEIFGFFPPSVNPPSKTPMLTLLKQARAYGLGVVVATQNPVDL
                     DYKGLSNTGTWWLGRLQTERDKARVLDGLEGAGASSGRPFDRGAMETTLSGLDRRVFI
                     MNSVHDGGPVKFQTRWALSYLRGPLTRAQIRRLTEGSAPPPVEPARPPGTPVAPAAGG
                     RSTAERPLLPAEIPQFFIEPDAGAVDDAMPLYRPALLGTAQLHYVRASDDIDLWRTIS
                     LLAPLPDRVTANVWSEAVVLERGEPPLAASGRDGARFGELPAAASRPKSFAVWGRSLA
                     ATLHRERKLSIRRCPALKSSSGPGETEAEFRIRLARLAREARDAAMTKLERRYAPKLA
                     RLRERIRRAEERIGRERSQYDQQKVQTAISIGATVLGALFGRKAASVGTVGRASTAMR
                     GVGRASREKGDVARAADALEAERAALAELDNEFRRDADAVRAGIDPLRIELERVEIAP
                     RKSDITIDRLALVWKPERSRGLR"
     gene            1421..>4646
                     /locus_tag="VD788_02190"
     CDS             1421..>4646
                     /locus_tag="VD788_02190"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXV75099.1"
                     /translation="MTTRCLLARLLLVACCAPLAAEESRLLRQPALGADRIAFVYGGD
                     LWTADLAGGDVRRLTSTPAVESDPHFSPDGSRIAFTSNRSGTPAAYLLPVDGGQPQRL
                     SWYPAPAYVRGFTPDGRRVLYASTRETAPDGYDRLWTVSVDGGPPERLPAPWGHDGSY
                     SPDGKRIVVDRVQRWDREWRHYRGGQNTPLVILDLATLDELRLPNDRTTDVRPVWLGD
                     TVYFLSDRDWVMNVWSYRPGSAELRQVTDFDRTDVKWLDGRDGTLIVEHDGYVHTLDP
                     ASGRTRKIPIELRGDFPWADPRWVEIGEEIRSASLSATGKRALFEARGEIFTVPAEKG
                     DPRNLTRSSGAADRAPVWSPDGGRVAWFSDAGDGYVLLIADQDGLADPQRIPIGASKM
                     AWDTTWSPDGSRIAFVDDDVRVRVVELETGEVTTADVGGANVERGEMGICWSPDSKWL
                     AYARSFPNRLRRIVVWSLDDGRARPITDSMADAFGPSWDRDGEHLYLLASTDVALGSG
                     WANTSRMGVDPTYAPYVVVLAAEQTTPFAPESDEEQVETDDEAGGDSQEQDEKKKKKK
                     KDEEDDEKEDEETPVVVRIDFDGIERRIIPLPIDRDRYTSTAAGPKGSVFIGRRVPGE
                     RDGTVHKFTLEDREEQEFLDGVRSFALSSDGTKLLYRNEESWFIVGTDKKPEAGDGKI
                     DVTLRARIDRLEEWRQIFDEAWRYQRDFFYDPAMHGRDWDDVRARYEPLVPHVRHRAD
                     LSYVLDQMNGELSVGHSFVFGGDMPQVETSRVGVLGADLVVDRGRFKIDRIYTFESWN
                     PEMKAPLDRPGLRVEVGHYIVAIEGSELTSADDPYRLLDGTADRQITLHVNDQPSMDG
                     AWTATVVPIGSEDDLRQRAWVEDNRRRVDELSGGRLAYVWVPNTGEPGVISFNRYFFA
                     QQDREGAVIDERFNGGGLLDDYMVDLMTRELRAAITNEVPDGEPFRLPAGILGPKVLL
                     INELAGSGGDFFPWVFRRQRAGPLIGTRTWGGLVKSSVHYPLVDGGALTAPDNAVFDP
                     AAGEWIAENAGIAPDIEVLLDAKSVADGRDPQLERGVRELLRLLD"
BASE COUNT          726 a         1561 c         1681 g          678 t
ORIGIN      
        1 acatggacga gatcttcggt tttttcccgc cgtcggtcaa cccgccgtcc aagacgccga
       61 tgctgacgct gttgaaacag gcgcgggcct acgggctggg agtggtggtc gcgacgcaga
      121 accccgtcga tctggactac aaggggctgt cgaacaccgg cacctggtgg ctcggacggt
      181 tgcagaccga gcgggacaag gcgcgcgtgc tcgacgggct cgaaggcgcc ggagcatcga
      241 gcggccggcc gttcgatcgc ggcgcgatgg agaccacgct ctccggtctc gaccgccgcg
      301 tcttcatcat gaacagcgtc cacgacggcg ggccggtgaa gttccagacg cgctgggcgc
      361 tcagttatct gcgcggcccg ctgacccgcg cgcagatccg ccggctgacc gagggcagcg
      421 ccccgccgcc cgtcgagccg gcgcggccgc cgggcacgcc cgtcgcgcct gcggccggcg
      481 ggcggtcgac ggccgagcgc ccgttgcttc cggccgagat ccctcagttc ttcatcgaac
      541 cggacgctgg cgcggtcgac gacgcgatgc cgctgtaccg gccggcgctg ctcgggaccg
      601 cgcagctgca ctacgtccgc gcgagcgacg acatcgacct gtggcgcacg atcagcctgc
      661 tggcgccgct gccggaccgt gtgacggcga acgtctggag cgaggccgtc gtgctcgagc
      721 gcggcgagcc gccgctcgcg gcgagcggtc gcgacggagc gcgcttcggc gagcttcccg
      781 ctgctgcatc gcggccgaag agcttcgcgg tctggggccg ctcgctggcc gccaccctcc
      841 accgcgagcg caagctgtcg atccggcgtt gtccggcgtt gaagtccagc tcggggccgg
      901 gcgaaaccga agccgagttc cgcatccgcc tcgcgcggct ggcgcgcgag gcgcgcgacg
      961 cggcgatgac gaagctcgag cggcgatacg cgccgaagct ggcacggctg cgcgagcgga
     1021 tccggcgagc ggaggagcgg atcgggcgcg agcgctcgca gtacgatcag cagaaggtgc
     1081 aaaccgcgat ctcgatcggt gcgacggtgc tcggcgcgct cttcggccgc aaggcggcca
     1141 gcgtcggcac ggtcgggcgt gcctcgaccg cgatgcgcgg cgtcggtcgg gcgtcacgcg
     1201 agaagggcga cgtcgcccgc gcggccgacg cgctcgaggc cgaacgcgcc gctctcgccg
     1261 aactggacaa cgagttccgc cgagatgccg acgcggttcg cgccgggatc gatcctctgc
     1321 ggatcgagct cgagcgggtc gagatcgctc cccgaaagtc cgacatcacc atcgatcgac
     1381 tggctttggt ctggaagccg gaacgatcac gaggtctgcg ttgacgactc gatgcctgct
     1441 cgcccgtttg ctgctcgtgg cttgttgcgc cccgctcgcg gccgaagaga gccgtctgtt
     1501 gcgccaaccg gcgctcggcg ccgatcgaat cgcgttcgtc tacggcggcg acctgtggac
     1561 cgccgatctc gccggcggcg acgtgcgccg cctgacgagc acgccggcgg tcgagagcga
     1621 cccgcacttc tcacccgacg gcagccggat cgcgttcacc tcgaaccgtt cgggcacgcc
     1681 cgcggcatac ctgctgccgg tcgacggcgg ccagccgcaa cgactcagct ggtatccggc
     1741 accggcctac gtccgcggtt tcacaccgga cggccggcgc gtgctctacg cctcgacacg
     1801 cgagacggcg cccgacggct acgaccggct gtggaccgtc tcggtcgacg gcggcccgcc
     1861 ggagcggttg ccggcgccgt gggggcacga cggcagctac tcgccggacg ggaagcggat
     1921 cgtcgtcgat cgcgtgcagc gctgggaccg cgagtggcgc cactaccgcg gcgggcagaa
     1981 tacgccgctc gtgatcctcg acctcgccac gctcgacgag ctgcggctgc ccaacgaccg
     2041 cacgaccgac gtgcgaccgg tctggctcgg tgacaccgtt tactttctct ccgatcgcga
     2101 ctgggtgatg aacgtctggt cctatcgacc cggttcggct gagctccgac aggtgaccga
     2161 tttcgatcga accgacgtca agtggctcga cggccgggac gggaccctga tcgtcgagca
     2221 cgacggctac gtccacacgc tcgacccggc ctcgggtcgg acgcgcaaga tcccgatcga
     2281 gctgcgcggc gattttccgt gggcggaccc gcgctgggtg gagatcggcg aggagatccg
     2341 ctcggcgtcg ctgtcggcga ccggcaaacg cgcgctgttc gaagcgcgcg gagagatctt
     2401 caccgtgccg gcggagaagg gcgacccgcg caacctgacg cgcagctcgg gtgcggccga
     2461 ccgcgcaccg gtctggtcgc ccgacggtgg ccgggtcgcc tggttctccg acgcgggcga
     2521 cggctacgtg ctgctgatcg cggatcaaga cgggttggcc gatccgcagc gcatcccgat
     2581 cggcgcgtcg aagatggcgt gggacacgac ctggtccccc gatggcagcc ggatcgcgtt
     2641 cgtcgacgac gacgtgcggg tacgggtcgt cgagctcgag acgggcgagg tcacgacggc
     2701 cgatgtcggc ggcgccaacg tcgaacgcgg ggagatgggg atctgctggt cgcccgattc
     2761 gaagtggctg gcctatgcac gcagctttcc caaccgcctg cggcgcatcg tcgtctggtc
     2821 gctcgacgac ggccgggcac gtccgatcac cgactcgatg gcggacgcct tcggtccgtc
     2881 gtgggatcgc gacggcgagc acctctacct gctggcgagc accgacgtcg cgctcggctc
     2941 cggctgggcc aacaccagtc gcatgggagt cgacccgacc tacgccccgt atgtcgtcgt
     3001 gctggccgcg gagcagacga cgccgttcgc gccggagagc gacgaggagc aggtcgaaac
     3061 cgacgacgag gccggcggcg acagccagga gcaggacgag aagaagaaga agaaaaagaa
     3121 ggacgaggaa gacgacgaga aagaggacga ggagacgccg gtcgtggtgc ggatcgactt
     3181 cgacgggatc gagcgccgga tcatcccgct gccgatcgac cgcgatcgtt acacgtcgac
     3241 cgcagccggg ccgaaggggt cggtgttcat cggccggcgc gtgccgggag agcgagacgg
     3301 gaccgttcac aagttcacgc tcgaggatcg cgaggagcaa gagttcctcg acggcgttcg
     3361 ctcgttcgcg ctgtcgagcg acgggacgaa gctgctgtac cgcaacgagg agagctggtt
     3421 catcgtcggc accgacaaga agccggaggc gggcgatggc aagatcgacg tgacgctgcg
     3481 cgcgcggatc gatcgcttgg aggaatggcg gcagatcttc gacgaagcgt ggcgctatca
     3541 gcgcgacttc ttctacgacc cggcgatgca cgggcgcgat tgggacgacg tgcgcgcgcg
     3601 ctacgagccg ctcgtgccgc acgtccgtca ccgcgccgac ctgagttacg tgttggacca
     3661 gatgaacggc gagctgtcgg tcgggcacag cttcgtcttc ggtggcgata tgccgcaggt
     3721 cgagacgtcg cgggtcggcg tgctcggcgc cgatctggtc gtcgatcgcg gccggttcaa
     3781 gatcgaccgg atctacacct tcgagagctg gaatcccgag atgaaagcgc cgctcgaccg
     3841 gcccggcctc cgggtcgaag tcggccacta catcgtcgcc atcgagggga gcgagctgac
     3901 ctccgcggac gatccgtatc ggctgctcga cggaaccgcg gatcggcaga tcacgctcca
     3961 cgtcaacgac cagccgtcga tggacggcgc ctggaccgcg accgtcgtac cgatcgggag
     4021 cgaggacgac ctgcgccagc gggcgtgggt cgaggacaat cggcgtcgcg tcgacgagct
     4081 gtccggcggc cggctggcct atgtctgggt gcccaacacc ggtgagccgg gcgtcatctc
     4141 cttcaatcgt tacttcttcg cgcagcagga ccgcgagggc gcggtgatcg acgagcgttt
     4201 caacggcggc gggctgctcg acgactacat ggtcgacttg atgacgcgcg agctgcgggc
     4261 ggcgatcacc aacgaggttc cggacggcga gccgttccgc ctgccggcgg ggatcctcgg
     4321 cccgaaggtg ctgctgatca acgagctcgc cggatcgggc ggcgacttct ttccgtgggt
     4381 cttccgccgg cagcgcgccg ggccattgat cggcacgcgc acctggggcg gactggtcaa
     4441 gtcgtcggtg cactatccgc tggtcgatgg cggagcgttg accgcgcccg acaacgccgt
     4501 gttcgacccg gccgccggcg agtggatcgc cgaaaacgcg ggcatcgcac cggacatcga
     4561 ggtgctgctc gacgccaagt cggtggccga cggccgcgac ccgcagctcg agcgcggggt
     4621 tcgcgagctg ctgcgcctgc tcgacg
//
LOCUS       DASPGN010000078         5189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_482543, whole genome shotgun sequence.
ACCESSION   DASPGN010000078 DASPGN010000000
VERSION     DASPGN010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482543"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="VD788_02195"
     CDS             complement(<1..295)
                     /locus_tag="VD788_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75100.1"
                     /translation="MNLPAVGVPEGYRRFDETGCTLIVRSEHAAAVRSAGFGRVDGWT
                     TALRRVGGSGRGRAVAVALEGGTELVLKRMCRGGAFASLWRGRFAGFRRLLDNL"
     gene            complement(292..1014)
                     /gene="tatC"
                     /locus_tag="VD788_02200"
     CDS             complement(292..1014)
                     /gene="tatC"
                     /locus_tag="VD788_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HXV75101.1"
                     /translation="MSILEHLDELRSRLVRVVVVFGVVLAASWAFSDAILEFLLIPIR
                     RHLLEGDEIVFITPVEPFMTYMKAAAILAVFVSAPYILYQTWAFVAPGLYRHERRMVV
                     PFLLFGTLFFFAGGAFGYYVATPVASGWLIGLGEKFTAQITLRSAFQFESRVILAMGV
                     VFELPVMIFFLTRIGIVTPGFLMRHFRTAVFVIAVLAAVLTPTGDVLTMAVFAGPMIV
                     LYLLGVGVSALVVRRPRGRSAS"
     gene            complement(1022..1375)
                     /gene="tatB"
                     /locus_tag="VD788_02205"
     CDS             complement(1022..1375)
                     /gene="tatB"
                     /locus_tag="VD788_02205"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HXV75102.1"
                     /translation="MFGSLGGAELLLILVLALLLFGPRRLPEIGRTIGRGMSEFRKAT
                     SEFKSNLEREVDLSEIRKTREGLADVRSAVGQAIDDGKRFAVPRSADPPGDSRRGRAE
                     GPAADTVDDEPRTDD"
     gene            1518..3194
                     /locus_tag="VD788_02210"
     CDS             1518..3194
                     /locus_tag="VD788_02210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXV75103.1"
                     /translation="MNQLTRRLPLLALVFLIAGGFIGGMIGSRALAEPQRPDDDLWTF
                     GRVLSLIEDQYVGEVDSKALIEDAVAGMLQSLDPHSNYLNPESFHEMRDEQRGKFSGL
                     GIQITKRGADKPLTIISPIDDTPADRAGLISGDVIARIEGAPTIEMTVQDAVRLLKGD
                     KGTRVTITIKRQGVPDPFDVTIERDDIPIESIRVAFMLDDQVGMIRVSNFTSTTADEL
                     DRAIAQLTEKGMTRLLVDLRGNPGGLLDQAVQVSERFIPADKLIVFTRGRIHGSDQDY
                     YAKRGVNRVDLPLVVLVDESSASASEIVSGAIQDHDRGLVVGEATFGKGLVQRVIPLQ
                     SGGALAVTTAKYYTPSGRLIQRDYSDLEEYYLGRAGEAGDDELPAPSPRDDEQTGEPT
                     EVEPTEVFYTASGRKVFGGGGIRPDYMVKAERAPDLLFDLLRENMIFDFAVRYNNEHA
                     ELDPEVPFDDAALADFERFLERVDFEYDRADLAEHKEIVRLRLRAQIARVRWDQNAEA
                     RILALADPQVQRAVGLFGEAAELSEAGADGEPGQPATPKDHLRAARDANE"
     gene            complement(3203..3889)
                     /locus_tag="VD788_02215"
     CDS             complement(3203..3889)
                     /locus_tag="VD788_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75104.1"
                     /translation="MIARQPPLLVTVQRVLERTYRMRSGIDDPGRFIIGDHGFTRFYR
                     DVEMPCSVSAASGEGATTIVRDSPTGPRVAVYLPDRLVATLERFPPQCGVFERNVDAF
                     ATLVEELDHLLLIAERAAVSRPVTMFELELHANVSKHLVLTRYLAGARSRLAPAARRW
                     LRHHLFDKVRYSEPDPSVRQRYRDARRWAVRLIDGLAGLDADDRIGLLRRFHREPTSG
                     KLSLIARLAA"
     gene            complement(3886..>5189)
                     /locus_tag="VD788_02220"
     CDS             complement(3886..>5189)
                     /locus_tag="VD788_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV75105.1"
                     /translation="RLDELATRVPLMAAAAAALEREADERDPSPIILSGTRDREARRN
                     AYLDGLRADAIDEIGISREQLLSSYERMLLFASLDPRPLREALDATVVDQQQATAALC
                     DEFSLFAAGTRDPRKPPAYFLVGPTGVGKNHLVETLGRLLTAAWGIEVPALTIEGPSY
                     TYPSDINELRGATRGFIRSDEEGLLTAFHQKSARAPLALILVDEVEKAHPQLLTFFLS
                     ILDRGTTTDNRGQLLNFANCMLFFTSNIGYSDAQQRSLPIGYGDDAERREKRDHEIRS
                     ELRRALKPEFVNRVRLIHFNRLTRASVDRILDLEMHRIAARYREMHGLEVVLDDSARA
                     ELIRRGFSETYGARRLSATLESVCNVELARKVRTDDRARHDKREALVEWLREMRDGKR
                     TFHVEEVKRRVVAQARARLGYRTVRIGYDGERFVYETGTPS"
BASE COUNT          929 a         1736 c         1752 g          772 t
ORIGIN      
        1 gcaggttgtc gagcaagcgc ctgaacccgg cgaagcggcc gcgccacagc gacgcgaacg
       61 ctccgccgcg acacatccgc ttgagcacca attccgtccc gccctcgagc gcgacggcga
      121 cggctcgtcc acggcccgaa ccgccgaccc ggcgcagagc ggttgtccaa ccgtcgacgc
      181 ggccgaagcc ggccgagcga acagccgcgg cgtgttccga ccggacgatc agcgtgcagc
      241 cggtctcatc gaatcgccgg tagccctcgg gaacaccgac ggccgggagg ttcacgatgc
      301 gctccgcccc cgcggacgac gcacgaccag cgcggaaacg ccgacaccca gcaggtagag
      361 cacgatcatc ggcccggcga agaccgccat cgtcaggacg tcgcccgtcg gcgtcagcac
      421 ggccgccagt acggcgatga cgaacaccgc cgtgcggaaa tggcgcatga ggaagccggg
      481 ggtgacgatg ccgatcctcg tcaggaagaa gatcatcacc ggcagctcga agacgacgcc
      541 catcgccagg atcacgcggc tctcgaactg aaacgccgag cgaagcgtga tctgcgcggt
      601 gaacttctcg ccgagaccga tcaaccagcc ggaggcgacc ggggtcgcga cgtaatagcc
      661 gaacgccccg ccggcgaaaa agaacagcgt tccgaacagc aggaacggga ccaccatcct
      721 ccgttcgtgc cggtacaggc cgggcgcgac gaaggcccaa gtctggtaga ggatgtacgg
      781 cgccgagacg aaaacggcga ggatcgccgc cgccttcatg tacgtcatga acggttcgac
      841 gggggtgatg aagacgatct cgtcaccctc gagcaggtgg cggcggatcg ggatcagcag
      901 gaactccagg atcgcgtcgc tgaacgccca cgacgcggcc agcacgacac cgaagacgac
      961 gaccactcga accagcctcg accgcagctc gtcgaggtgc tcgaggatcg acatcgagga
     1021 ttcagtcgtc ggttcggggc tcgtcatcga ccgtgtccgc cgcggggcct tccgcccggc
     1081 cgcggcgcga gtcgccgggt gggtcggcgc tccgcggcac ggcgaaccgt ttaccgtcgt
     1141 cgatcgcttg accgaccgcc gagcgaacgt ccgcgagacc ctcgcgcgtc ttgcggatct
     1201 cgctcagatc gacttcgcgc tcgaggttgc tcttgaactc ggaggtcgcc ttgcgaaact
     1261 ccgacatccc gcggccgatc gtacggccga tctcgggcag ccggcgcggc ccgaacagca
     1321 gcagggccag cacgaggatc aacagcaact cggcgccgcc gagcgaaccg aacatccgca
     1381 accctccggg accgggcgac cgggtggagc gtactgcccc gttccggggg tgtcaagaaa
     1441 ccggccgcgt ttcggttgtc gcaaatctag gccgatcata taattaggtg tcggtctgga
     1501 gagatccgga gagaaaaatg aaccaactga cccgccgcct tccgctgctc gctctggttt
     1561 tcttgattgc cgggggtttc atcggtggga tgatcggctc tcgggcgctc gccgaaccgc
     1621 agcggccgga cgacgacctc tggaccttcg gtcgcgtgct gtcgctgatc gaagaccagt
     1681 acgtcggcga ggtcgactcg aaggcgctga tcgaagacgc cgtcgccggc atgctgcagt
     1741 cgctcgaccc gcacagcaac tacctcaacc ccgaatcgtt ccacgagatg cgcgacgaac
     1801 agcgcggtaa gttctcgggt ctcggcatcc agatcaccaa gcgcggcgcg gacaagccgc
     1861 tgacgatcat ctcgccgatc gacgatacac cggccgaccg cgccgggttg atctcgggcg
     1921 acgtgatcgc ccgcatcgag ggagctccga cgatcgaaat gacggtgcag gacgcggtcc
     1981 gcttgctcaa gggcgacaag gggacccgtg tcacgatcac gatcaagcgc cagggcgttc
     2041 ccgacccgtt cgacgtgacg atcgagcgcg acgacatccc gatcgagagc atccgggtcg
     2101 ccttcatgct cgatgaccag gtcggcatga tccgggtgtc gaacttcacc tcgacgacgg
     2161 cggacgagct ggaccgagca atcgcccagc tgaccgaaaa gggcatgacg cgtctgttgg
     2221 tcgacctgcg cgggaacccc ggtgggttgc tcgaccaggc ggtccaggtc tccgagcgct
     2281 tcatacccgc cgacaagttg atcgtcttca cccggggccg gatccacgga tcggatcagg
     2341 actactacgc caagcgcggc gtcaaccgcg tcgatctgcc gctggtcgtg ctcgtcgacg
     2401 agagctcggc gtcggccagc gagatcgtct cgggcgcgat ccaggaccac gaccgcggcc
     2461 tggtcgtcgg cgaagcgacg ttcggcaagg gtctggtcca gcgcgtcatc ccattgcagt
     2521 cgggcggcgc gctggccgtc accacggcga agtactacac gccgtcgggc cggctgatcc
     2581 agcgtgacta ctcggacctc gaggagtact acctcggtcg cgccggcgag gcgggcgacg
     2641 acgagctgcc cgcgccgtcg cccagagacg acgagcagac cggggagccg accgaggtgg
     2701 agccgaccga ggtgttctac accgcctccg ggcgcaaggt gttcggcggc ggcggcatcc
     2761 gtcccgacta catggtgaag gccgagcgcg ctccagacct gctgttcgat ctgttgcggg
     2821 aaaacatgat cttcgatttc gcggtgcgct acaacaacga gcacgccgag ctcgaccccg
     2881 aggtgccgtt cgacgacgcg gcgctggccg atttcgagcg cttcctcgag cgggtggact
     2941 tcgaatacga ccgggccgac ttggccgagc acaaagagat cgtccggctg cgcttgcgcg
     3001 cgcagatcgc ccgcgtgcgt tgggatcaga acgccgaagc gcgtatcctg gccctggcgg
     3061 acccgcaggt acagcgtgcg gtcgggctgt tcggcgaagc ggcggagctg tccgaggccg
     3121 gcgccgacgg cgagccgggg caacccgcca cgccgaaaga ccatctgcgc gcggccaggg
     3181 acgcgaacga gtagaccgga gctcaggccg cgagccgagc gatcaacgag agcttgcccg
     3241 acgtcggctc gcgatggaac cgtcggagca ggccgatgcg atcgtcggcg tcgagaccgg
     3301 caagaccgtc gatcaaacgc accgcccagc ggcgggcgtc acggtagcgc tggcgcaccg
     3361 acgggtccgg ttcgctgtaa cgcaccttgt cgaacagatg gtggcgcagc cagcgacggg
     3421 ccgcgggggc gagccgcgac cgcgctccgg ccaggtaacg cgtcagcacg aggtgcttgc
     3481 tcacgttcgc gtgcagttcc agctcgaaca tcgtcaccgg acgcgaaacg gccgcccgct
     3541 cggcgatcag cagcaggtga tccaattcct cgaccaaggt cgcaaacgcg tcgacgttgc
     3601 gctcgaagac gccgcactgc ggtgggaacc gttcgagcgt cgcgaccagg cgatccggca
     3661 agtagaccgc cacgcgcggg ccggttggag aatcgcggac gatcgtcgtc gctccctctc
     3721 ccgacgcggc cgacaccgag cacggcattt cgacgtctcg gtagaagcgc gtgaatccgt
     3781 gatcgccgat gatgaagcgg cccgggtcgt cgatacccga tcgcatgcga taggtgcgct
     3841 cgagcacgcg ctggacggtt acgagcagcg gcggctggcg agcgatcacg acggcgtccc
     3901 tgtttcgtag acgaagcgct cgccgtcgta gccgatgcgg acggtacggt atcccagccg
     3961 ggcgcgggcc tgggccacga cccgccgttt gacttcctcg acgtggaacg tgcgcttgcc
     4021 gtctcgcatc tcccgcagcc attcgacgag tgcttcgcgt ttgtcgtgcc gagcccgatc
     4081 gtcggtgcgc accttgcgcg ccagctcgac gttgcaaacc gactcgagcg tcgccgacag
     4141 gcgccgcgcg ccgtaggtct cggagaagcc tcgtcgaatc agctcggccc gcgcgctgtc
     4201 gtccaacacg acctcgagac cgtgcatctc gcggtaacgc gccgcgatgc ggtgcatctc
     4261 gaggtcgagg atacggtcga cgctcgcgcg agtcagccgg ttgaagtgga tcaaccggac
     4321 gcggttgacg aactccggct tcagcgcgcg gcgcagctcg gagcgtatct cgtggtcgcg
     4381 cttctcgcgc cgctcggcgt cgtcgccgta gccgatcggc agcgaccgct gctgggcgtc
     4441 gctgtagccg atgttggagg tgaagaacag catgcagttg gcgaagttga gcagctggcc
     4501 gcggttgtcg gtcgtcgtgc cgcgatcgag gatcgacagg aagaaggtca acagctgcgg
     4561 atgcgccttc tctacctcgt cgaccaggat cagcgcgagc ggggcgcgcg ccgacttctg
     4621 gtggaaggcc gtcagcagcc cctcttcgtc ggagcggatg aaaccgcgcg tcgctccgcg
     4681 cagctcgttg atgtcggacg gataggtgta gctcggacct tcgatggtca acgccggcac
     4741 ctcgatgccc caagccgcgg tcagcagtcg ccccaacgtc tcgacgaggt ggttcttgcc
     4801 gacgccggtc ggtccgacga ggaagtaggc cggtggtttg cgcgggtcgc gggtgccggc
     4861 ggcgaacagg ctgaattcgt cgcacaacgc cgcggtcgcc tgttgctgat cgacgaccgt
     4921 ggcatcgagt gcctcacgca gcgggcgcgg gtcgaggctg gcgaacaaca gcatccgctc
     4981 atacgaagac agcagctgtt cgcgcgaaat gccgatctcg tcgatcgcat cggcacggag
     5041 cccgtcgaga taggcgttgc gcctcgcctc gcgatcccgt gtccccgaca ggatgatcgg
     5101 cgacggatcc ctctcgtcgg cttcgcgctc gagtgccgcg gccgccgccg ccatcaacgg
     5161 gacacgggtc gccagctcgt cgagccgcc
//
LOCUS       DASPGN010000079         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1536431, whole genome shotgun sequence.
ACCESSION   DASPGN010000079 DASPGN010000000
VERSION     DASPGN010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1536431"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VD788_02225"
     CDS             complement(<1..1244)
                     /locus_tag="VD788_02225"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXV75106.1"
                     /translation="MRRTIGMLLALATWLGGCAPDDPATRLDQLIEREWLARLTEFPE
                     FATSVGVHERNHLLTSMTIDDLARRDKMWRGLLEQLDEIDPGRLTTEDRIDYEMLRDQ
                     LEERTESFRFGDYQIPINSESGFHTGFARLPFDVPLDDADDYDDYIARMRAWPALVDQ
                     QITLMRIGLDRGMTPPRVVLAGVERTMEAQLVEEPRDSDFYAPFRAFPVGVSAAEHER
                     LERAGRAAIAESIVPGYRALRDFFVGEYVPAARETLGASALPDGAVYYAWRVRWYTTL
                     DIDADEIHELGLSEVARIRAEMTGVIARTGFEGSFDEFLTFLREDPRFYAKTPQQLLE
                     RAAWITKRMDGELPALFGRLPRLPYTVEPVPDGIAARYTAGRYVGAPVGSSQPGRYWV
                     NTYALETRPLYTLEALSLHEAVP"
     gene            1355..1762
                     /locus_tag="VD788_02230"
     CDS             1355..1762
                     /locus_tag="VD788_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75107.1"
                     /translation="MRHGFHLLSAVAVLAFAYGGGSAAEAVTLEGGFVWERDDGTSDG
                     ALTAVLTPDGDGKWNVAFHFDWEDGAHVYSGTCEGSLTGNLAGEVQSDNQDNTMNFRF
                     SGKFENGTFNGTHAFITKEGEVKDSGTLTLAAK"
     gene            1802..>2597
                     /locus_tag="VD788_02235"
     CDS             1802..>2597
                     /locus_tag="VD788_02235"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV75108.1"
                     /translation="MRDSRWDEIDGWFARALECPPAERAGFLAGCPDREVRDRVERLL
                     SAAERSGDLLSSGAIPLIAGASSQPANESVRRVFARGRALLDRYRVDGEIGAGGFGVV
                     YRARDLRLEREVAIKVVAGPQVNRERLLREARAAAATNHPNLVGVFDVGQVDGLPFLV
                     MELIEGPTLEESPPRTIDEAVRVAIEICRGLEHMHDRGMVHRDLKPCNVLRVRHDHGA
                     TIKLVDLGLVRSDRSAALTAEGHLLGTPAYLAPEQARGGSIDPRTDL"
BASE COUNT          413 a          862 c          932 g          390 t
ORIGIN      
        1 gggacggcct cgtgcagcga caacgcttcg agtgtgtaca acggccgcgt ctcgagggcg
       61 taggtgttga cccagtaacg tccgggctgg ctgctgccga ccggggcgcc gacgtagcgg
      121 cccgcggtgt agcgggcggc gatgccgtcg ggcaccggct cgacggtgta gggcagtcgc
      181 ggcagccggc cgaacagcgc cggcagctcg ccgtccatcc gtttggtgat ccaggccgcg
      241 cgctcgagca gctgctgggg cgttttggcg tagaaccgcg ggtcctcacg caggaaggtc
      301 aggaactcgt cgaagctccc ctcgaagccg gtccgcgcga tcaccccggt catttcggcg
      361 cggatgcgcg cgacctcgct cagccccagc tcgtggatct cgtcggcgtc gatgtcgagc
      421 gtcgtgtacc agcgcacgcg ccacgcgtaa tacaccgcgc cgtccggaag cgcggaggca
      481 ccgagcgttt cgcgtgccgc gggcacgtac tccccgacga agaaatcgcg caaggcgcga
      541 tagcccggca cgatcgactc ggcgatcgcc gcgcgcccgg cgcgctcgag ccgctcgtgc
      601 tccgcggcac tgacgccgac ggggaacgca cggaacggcg cgtagaaatc gctgtcgcgc
      661 ggctcttcga cgagctgcgc ctccatcgtg cgctcgacac cggcgagcac gacccgtggc
      721 ggcgtcatcc cgcgatcgag gccgatccgc atcaacgtga tctgctgatc gaccagcgcc
      781 ggccaggcgc gcatgcgcgc gatgtaatcg tcgtagtcgt cggcgtcgtc gagcggaacg
      841 tcgaacggaa ggcgagcgaa gccggtgtgg aaaccggact cggagttgat cgggatctgg
      901 taatcgccga agcggaacga ctcggtgcgc tcctcgagct ggtcgcgcag catctcgtag
      961 tcgatccgat cctcggtcgt caaccggccc gggtcgatct cgtcgagctg ttcgagcaac
     1021 ccgcgccaca tcttgtcgcg tcgcgcgaga tcgtcgatcg tcatcgaggt caacagatgg
     1081 ttgcgctcgt ggaccccgac cgacgtcgcg aactcgggga actcggtcaa ccgagcgagc
     1141 cattcccgct cgatgagctg gtcgagccgg gtcgcagggt cgtccggcgc gcagccgccg
     1201 agccaggtcg ccagcgccaa cagcatcccg atcgtccgac gcatggggcc tccgtcgtcg
     1261 cggcggcgat cgtacaccgg gtggggccgg tttgccgcga gcggggcgaa gtgcgatcat
     1321 ctgccgcaca aggggtcata accggaggat tcgcatgaga cacggttttc atctgctttc
     1381 ggccgtggcg gtcttggcgt tcgcctacgg aggcgggtcg gctgccgagg cggtcacgct
     1441 cgagggcggg ttcgtttggg agcgcgacga cggtacgtcc gacggtgcgc tgacggcggt
     1501 cctcacgccc gacggtgacg gcaagtggaa cgtcgcgttc cacttcgact gggaagacgg
     1561 ggcacacgtc tatagcggga cgtgcgaggg aagcctgacc ggaaatttgg ccggagaggt
     1621 ccagagcgac aaccaggaca acacgatgaa cttccgcttc agcggcaagt tcgaaaacgg
     1681 caccttcaac ggtacgcacg cgttcatcac caaggaaggg gaggtgaagg acagcggaac
     1741 gttgacgctg gccgccaagt aagcgcgccg gctcggttcc gtcgaccccg cgagcggcca
     1801 cgtgcgagat tcacgctggg acgagatcga cggctggttc gcccgcgcgc tcgagtgtcc
     1861 gcccgccgag cgcgcgggtt tcctcgccgg ttgccccgac cgcgaggtgc gcgatcgcgt
     1921 cgagcgactg ctgtcggcgg cggaacggtc gggcgacctg ctcagcagcg gcgcgatccc
     1981 gctgatcgcg ggggcgagca gccagcccgc gaacgagtcg gtgcggcgcg tgttcgctcg
     2041 cggccgggcg ttgctcgacc gctaccgcgt cgacggcgag atcggcgccg gcgggttcgg
     2101 cgtcgtctac cgcgctcgcg acctccggct cgagcgcgag gtcgcgatca aggtcgtggc
     2161 cgggccgcag gtcaaccgcg aacggctgct gcgcgaggcg cgtgcggccg ccgcgacgaa
     2221 ccacccgaac ctggtcggcg tcttcgacgt gggccaggtc gacggcctgc cgttcctcgt
     2281 gatggagctg atcgaagggc cgacgctcga ggagtctccg ccgcggacga tcgacgaggc
     2341 ggtgcgcgtc gcgatcgaga tctgccgcgg gctcgagcac atgcacgacc gcgggatggt
     2401 ccatcgagat ctcaaaccgt gcaacgtgct gcgtgttcgg cacgaccacg gggcaacgat
     2461 aaagctggtc gacctcggtc tggtgcgctc cgaccgaagc gcggcgctga ccgccgaggg
     2521 acacctgctc ggtacgccgg cgtacctggc tcccgagcaa gcgcgcggcg ggtcgatcga
     2581 cccccgcacc gacctct
//
LOCUS       DASPGN010000080         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_249298, whole genome shotgun sequence.
ACCESSION   DASPGN010000080 DASPGN010000000
VERSION     DASPGN010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249298"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VD788_02240"
     CDS             complement(<1..1136)
                     /locus_tag="VD788_02240"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXV75109.1"
                     /translation="MLAVAASVSALAVAELPRLRLAADLRELLPETDPAARAYRLLLD
                     RFGALDRVYVVVRLAGEVDDGGELLAEAACEIASALERSEWFASARCGPTERDHDFLL
                     ESVLPRAALLIPPDRLASHVRPEAIRERAAWMRAQLAGPLGFVEAPFFAADPLGLAAE
                     LTALSDGHAGLAVDPVSGVFLSRSGTRALVVATPRIAELDTAAGREMVRFLDETFREA
                     GRAENGAIEFGALGGPLYAVHDETVIRGDVTRTAFGAAAGIAILLLLYFGSPRIPGIL
                     LVSVSAGIVWTAALMTWVRGEISVIGVSFAAILIGLGVDYGIHGASAFRETAAGPGQA
                     GRAMMHVFATSGPAIVASAATTAAGFLVLVLADFWPVRELGLLVA"
     gene            complement(1183..1911)
                     /locus_tag="VD788_02245"
     CDS             complement(1183..1911)
                     /locus_tag="VD788_02245"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV75110.1"
                     /translation="MPGDTILVTGGNRGIGRSIVRALARSGSTVAFTYRTDRSAAREL
                     EDELGRTVRAFPLDLTDPEGPSRVVTSVEEELAAIGGLVNNAGSTTSGLLAMTPDAEW
                     QRVLDTNLGGVFRCCRAVLPLLMHRRRGSIVNIVSLGAIHGVAGQSVYAASKAGAIGL
                     TRSLAREVGKRNVRVNAVAPGFVRTDLTAKLSDEQIAVLRAAECLPDGVRVESVAETV
                     CFLLSDAAASITGQCVIVDAGVSA"
     gene            complement(1887..2594)
                     /locus_tag="VD788_02250"
     CDS             complement(1887..2594)
                     /locus_tag="VD788_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV75111.1"
                     /translation="MSAGATSIAVTVPAYQAAPSVGEVVRRCRLVSDQVLVVDDGSTD
                     GTAAVSAAAGARVVRHPRNLGKGRALRTAFDDLFARGFDRVVTLDADGQHLPEEVPKL
                     LGIAAGADLVIGSRDHLFGEMHPVRRMSNRCSSYLVSAVAGRPSPDVQSGFRLYSRRL
                     IDAVGFPEPRFEAESAIVVRAVRGGFRVRSVPIALGFADGRVTSHYRPLIDSLRIAIA
                     VARARMESAGCLATRSS"
     gene            complement(2591..>2861)
                     /locus_tag="VD788_02255"
     CDS             complement(2591..>2861)
                     /locus_tag="VD788_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75112.1"
                     /translation="VLRCAWNGAPLPAVLAPKSVVGEYGGGLLAAAVLIAGGVCPGPT
                     SGFETPDPELGVVPHDGSPLPPLRRVLVSALAAGGAASWLILERG"
BASE COUNT          466 a         1053 c         1021 g          321 t
ORIGIN      
        1 gcgacgagca ggcccagctc gcgcaccggc cagaagtcgg ccagcaccag caccaggaac
       61 ccggccgcgg tcgtcgctgc ggaggcgacg atcgccggcc ccgacgtcgc gaacacgtgc
      121 atcatcgccc ggccggcttg ccccggcccg gccgccgtct cgcggaacgc cgacgctccg
      181 tggatgccgt agtcgacgcc gaggccgatc aggatcgctg cgaacgaaac gccgatgacg
      241 ctgatctctc cgcggaccca cgtcatcaat gccgcggtcc agacgatccc ggccgagacc
      301 gaaacgagca ggattccggg gatgcgcggg ctgccgaagt agagcagcag caggatcgcg
      361 atgccggccg cggcgccgaa cgccgtgcgg gtgacgtcgc cgcggatcac cgtctcgtcg
      421 tggaccgcgt agagcgggcc gccgagcgcg ccgaactcga tcgcgccgtt ttcggcccga
      481 ccggcctcgc ggaacgtctc gtccaggaag cgcaccatct cccggccggc ggcggtgtcg
      541 agctcggcga tcctcggcgt ggcgacgacg agcgcgcgcg tccccgatcg cgagaggaag
      601 accccgctga ccggatcgac ggcgagcccg gcgtgcccgt cggacaaggc cgtcagctcg
      661 gcggcgaggc cgagcgggtc cgccgcgaag aacggggcct cgacgaagcc gagcgggccc
      721 gcgagctggg cccgcatcca cgccgcgcgc tcccgaatcg cctccggccg gacgtggctc
      781 gccagccggt cgggggggat cagcagggcc gcgcgcggca gcacggactc gagcaggaag
      841 tcatggtcgc gctcggtcgg cccgcagcgc gcggaggcga accactcgga ccgctcgagg
      901 gccgaggcga tctcgcatgc cgcttcggcg agaagctcgc cgccatcgtc cacctccccg
      961 gcgagacgaa cgacgacgta gacccggtcg agggcgccga accggtcgag cagcaaccgg
     1021 taggcgcgcg cggccgggtc ggtttccggc aacagctccc tcagatcggc ggcgagccgc
     1081 aaccgcggca gctcggcgac cgcgagggcg gagaccgacg cggcgaccgc cagcatgacg
     1141 agccatggtc gaggtgacgc ggcggagaca cgggcgcgca tctcacgcgg agacgccggc
     1201 gtcgacgatc acgcactggc cggtgatgga cgccgccgcg tcggacagca ggaagcacac
     1261 ggtctcggcc accgattcga cgcgcacccc gtcgggcagg cattcggccg cgcgcaacac
     1321 ggcgatctgt tcgtcggaca gcttcgcggt caggtcggtc cggacgaaac cgggcgcgac
     1381 ggcgttgacc cgcacgttgc gtttgccgac ctcgcgggcc agcgagcgcg tcagcccgat
     1441 cgccccggcc ttcgacgccg catagacgct ctgccccgcg acgccgtgga tcgcgccgag
     1501 cgagacgatg ttgacgatgc tgccccgccg gcggtgcatc aacagcggga gcaccgcgcg
     1561 acagcaacgg aacaccccgc cgagattcgt gtcgaggacc cgctgccatt cggcgtccgg
     1621 ggtcatcgcg agcagcccgc tcgtcgtcga gccggcgttg ttgaccagtc cgccgatcgc
     1681 ggccagctcc tcctcgacgg aagtgacgac gcgactcggt ccctccggat cggtcaggtc
     1741 gagcgggaag gcgcgcacgg tacgacccag ctcgtcctcg agctcgcgcg ccgccgaacg
     1801 gtccgtgcgg taggtgaacg cgaccgtcga tccggatcga gcgagagcgc gaacgatcga
     1861 acgaccgatg cctcggttcc cgccggtcac gaggatcgtg tcgccaggca tcctgctgac
     1921 tccatccgcg ctcgagccac ggcgatcgcg atgcgcaggc tgtcgatcaa cggccggtag
     1981 tggctcgtca cccggccgtc ggcgaagccc agcgcgatcg gcacggagcg cacgcggaag
     2041 ccgccgcgca ccgcgcggac cacgatcgcg ctctcggcct cgaatcgagg ctcgggaaac
     2101 ccgaccgcgt cgatcagccg tctggagtat aagcggaaac cgctctgcac atcgggcgac
     2161 ggccggccgg cgacggccga gacgagatac gacgagcagc ggttactcat ccggcggacc
     2221 ggatgcatct cgccgaacaa gtgatcgcgg ctgccgatga ccaggtccgc gccggcggcg
     2281 atcccgagga gcttcgggac ctcctccggt aggtgctggc cgtcggcgtc gagcgtgacg
     2341 accctatcga agccccgcgc gaacagatcg tcgaacgcgg tgcgcagcgc gcgccccttg
     2401 cccagattcc gcggatgacg gacgacccgt gcgccggcgg cggcggagac cgccgcggtg
     2461 ccgtcggtcg agccgtcgtc gacgaccagc acctggtctg agaccagccg gcagcgccgc
     2521 accacctcgc cgacggaggg cgccgcctgg taggccggaa cggtgacggc gatcgacgtc
     2581 gcacccgcgc tcatcctcgc tccaggatca accacgacgc cgccccgccc gcggcgagcg
     2641 cggagaccag caccctgcgc aacggcggca gcggcgagcc gtcgtgcggg acgactccca
     2701 gctcgggatc cggggtctcg aaacccgagg tcggtccggg gcagacgccg ccggcaatca
     2761 ggaccgccgc ggcgagcaat ccgccgccgt actcgccgac gacggacttg ggggcgagca
     2821 ccgcgggcag cggcgcgccg ttccaggcgc accgcagcac c
//
LOCUS       DASPGN010000081         5458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1653943, whole genome shotgun sequence.
ACCESSION   DASPGN010000081 DASPGN010000000
VERSION     DASPGN010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653943"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VD788_02260"
     CDS             <1..718
                     /locus_tag="VD788_02260"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXV75113.1"
                     /translation="QHYEIGARHYRNVVGFRRGLVPGAAVRVIGAHYDALGPGPAADD
                     NASGVAALLELVRSRPAVPPRRSQYFVAFGGGEHTDAAGTGSRHFARALRSDGIEVDL
                     MLALDGVGHFTDAPGSQRFPFPGLGLLYPDRGNYLVIAGDLGSGRVLKRVRGAIESMT
                     DLPVHTVRFPSAVWGGMGSDHRAFRELGMPGVLVTDLPELRVPWHGAEQDTADKLDYE
                     RMAEVVRGLHSVVLNRDVAD"
     gene            851..2275
                     /locus_tag="VD788_02265"
     CDS             851..2275
                     /locus_tag="VD788_02265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV75114.1"
                     /translation="MNSASPLVDDRFLVLDKLGQGGMAAVFRAFDRVEQRYVALKVHR
                     EAGAPGPSHPLAAEYDVWSRMRHRHVVRAYELAVAASGPFAPETPYLVLECASDTDPR
                     PTASDAALPATEVERLGTQLLRAMHHVERTGWVHRDIKPGNILAVGQGARRVYKLTDF
                     GLAVERGRREPIGRVSGSLPYVAPECLLGRALDARTDLYALGVTLFRLATGRLPAPAT
                     DPAALVGWHLWGAPADPRELLPAFPPRLARFVRRLTQRDPGERPAGAADALALLGATP
                     SGHDRPPLPLDNATRAELRLALDAVRLGASREFRLDRRELPASELARQLRSWSHVFGL
                     GFADLTTGIDPAVVLRRELLRLLIEMRDQAAPAIRLHRLDRAMGITCVAGQPVVDHSR
                     RLERWAETEGIVSFLATRARRRGLVLVAAAPHAGCPLTARVVDRLRARLRGARPAPGC
                     RGLLLVLTGVSETATARGSSDRAG"
     gene            complement(2229..3245)
                     /locus_tag="VD788_02270"
     CDS             complement(2229..3245)
                     /locus_tag="VD788_02270"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXV75115.1"
                     /translation="MLVLMHESLVPPESIEGLSDKELAPFKTEFDVTSTLREMGHEVL
                     PLGVDTDLGVLRQAMEQFNPQITFNLLEEFHGVAIYDQHVVSYLELMKRHYTGCNPRG
                     LMLAHDKALSKQVMAFHRISVPDFAVYPMNRSFREPPKRLAFPLLVKSLTAEGSVGIA
                     QASLVHDAKQLRERTEFVHRQTGTDAIAERYIDGRELYVGVWGNRRLDTLPVWELRYA
                     DPRRAAPLIATAKIKWDWAYQQRSGIVYGPADDLDEALGERIRRTCKRAYRVLNLTGY
                     ARIDLRVTDDGRIYVLEANPNPDLSYGGEFAESAETAGIAYDDILDRIIQLGLSYRAQ
                     WQSR"
     gene            complement(3302..4282)
                     /locus_tag="VD788_02275"
     CDS             complement(3302..4282)
                     /locus_tag="VD788_02275"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HXV75116.1"
                     /translation="MLATRICDLGLKVDGTQLERLVKRLYAELDERGLCFRPAVWLSS
                     EWFSPDGVPGIAIPFYLAHRRLVALEAKQMLEVEGGNDRSCMRVLRHEAGHAIDSAYR
                     LHFRKGWRQTFGRYSEPYPDYYRPKPNSRNYVLHLDAWYAQAHPAEDFAETFAVWLKP
                     RSHWRSHYRDWPAALRKLEYVDRTMDEIAGKPPVVLRRERVEPVSQLKQTLGEYYDKK
                     RERYGVQWSDFYDRDLRRLFSDDRHYAARPTASSFLRSSRKKIRETVSEWTGAHAYTI
                     DQVLQEMIERCRELKLRVAVPAHEARSGALLLLTKHTMHRVHVGKHEIPL"
     gene            complement(4424..4837)
                     /locus_tag="VD788_02280"
     CDS             complement(4424..4837)
                     /locus_tag="VD788_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75117.1"
                     /translation="MGNKVRRVQYFYATVKDEPGAAYRLLTQVASAEVNLLAFRAIPM
                     GPGVTQLVLFPDRVDQLARAAEQNGVVLTGPHDAFLIQGDDRMGALVDWHQRLTDARV
                     NVYASDGVTDGHGHYGYVLYVRPEDYEAAATVLGV"
     gene            complement(4907..>5458)
                     /locus_tag="VD788_02285"
     CDS             complement(4907..>5458)
                     /locus_tag="VD788_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75118.1"
                     /translation="RKLPDTRQSVTHKFSVAGHEGYITVGLYEDGKPGEIFITLGKAG
                     STLAGFADAFATAISFALQHGVELRFLVDKFAHVRFEPSGFTGNSRIPIAKSIIDYVF
                     RWLALQFLPAEDQPVKASEPVNGSEIEPDPQAAAPDELRDRERAVYVAQADAPPCHDC
                     GEIMIRNGACYACVNCGATSGCS"
BASE COUNT          777 a         1930 c         1875 g          876 t
ORIGIN      
        1 ccagcactac gagatcggcg cacggcacta ccgcaacgtc gtcggcttcc gacgcggcct
       61 cgtccccgga gcggcggtcc gcgtgatcgg cgcgcactac gacgcgctcg gaccgggccc
      121 cgccgcggac gacaacgcga gcggcgtcgc cgcgctgctc gaactggtcc gcagccggcc
      181 ggccgtgccg ccgcgtcgca gccagtactt cgtcgccttc ggcggcggcg agcacaccga
      241 cgcggcggga acgggaagtc ggcacttcgc ccgcgcgctg cgcagcgacg gcatcgaggt
      301 cgacctgatg ctggcgctcg acggcgtcgg ccatttcacc gacgcgcccg gctcccaacg
      361 gttccccttc cccgggctcg gcctgctcta cccggatcga ggaaactacc tcgtcatcgc
      421 cggcgacctc ggttcgggcc gcgtgttgaa gcgcgttcga ggcgcgatcg agtcgatgac
      481 cgacctcccg gtccacaccg ttcgcttccc gtcggccgtc tggggcggga tgggctccga
      541 tcatcgggcg tttcgcgagc tgggcatgcc gggggtcctc gtcaccgacc tgcccgagct
      601 gcgagtcccg tggcacggcg ccgagcagga cacggcggac aagctcgact acgagcggat
      661 ggccgaggtg gtgcgcgggc tgcactcggt cgtgctcaat cgcgacgtcg cggactgagc
      721 gccgcggttc ccgctcgaca acgccctccc gcgcccggcg caaccggacg gctcgcctgc
      781 ggttgccgaa cccgacccgg tgaccctaga ctccgcgagc gttcccgtcc gcccgcaccg
      841 caccatgctc gtgaacagcg cttcacccct ggtcgacgat cgcttcctgg tgctcgacaa
      901 gctcggccag ggcggcatgg ccgcggtctt ccgggcgttc gaccgggtcg aacaacgcta
      961 cgtcgctctc aaggtccatc gcgaagccgg cgctcccggc ccgtcgcatc cgttggcggc
     1021 ggaatacgac gtctggagtc ggatgcgcca ccgccacgtg gtccgcgcgt acgagctggc
     1081 cgtcgccgcg agtggcccgt tcgccccgga gacgccgtat ctggtgctcg aatgcgcgtc
     1141 cgacaccgat ccgcgcccga ctgcgagcga cgccgcgctc cccgcgaccg aggtcgagcg
     1201 cctcggcacc cagctgctcc gcgcgatgca tcacgtcgag cgcaccgggt gggtccaccg
     1261 cgacatcaag ccgggcaaca tcctcgcggt cggccaaggc gcccggcggg tctacaagct
     1321 gaccgacttc gggctcgccg tcgagcgcgg tcggcgcgag ccgatcggac gggtcagcgg
     1381 ctcgctgccc tacgtcgcgc cggagtgcct tctcggtcga gcgctcgacg cgcgcaccga
     1441 cctgtacgcg ctcggcgtga ccctgttccg gctcgcgacc ggccgccttc ccgccccggc
     1501 gaccgacccg gcagcgctcg tcggctggca cctgtggggc gcgccggccg atccgcgcga
     1561 gttgcttccc gcctttccgc ctaggctcgc ccgcttcgtt cgacggttga cccagcgcga
     1621 ccccggggag agacccgcgg gagcggccga cgccctcgcc cttctcggcg cgaccccctc
     1681 cggccacgac cgtccacctt tgccgctcga caacgcgaca cgcgccgagc tccggctggc
     1741 gctcgacgcg gtgcgtctcg gcgcgtcgcg cgagttccgc ctcgatcggc gtgagctgcc
     1801 ggcgagcgag ctggcgcgac agctccgcag ttggtcccat gtgttcggtc tcggcttcgc
     1861 cgacctcacg accggcatcg accccgcggt cgtcctgcgt cgcgagctac tgcgactgct
     1921 gatcgagatg cgggaccagg cggcaccggc gattcgattg caccggctcg accgcgcgat
     1981 gggcatcacc tgcgtcgccg gccaaccggt cgtcgaccac tcgcgccggc tcgaacgatg
     2041 ggcggaaacc gaggggatcg tctcgtttct cgcgacccgc gcccgccgtc gcgggctggt
     2101 gctcgtcgcc gcggcgcctc atgccggctg cccgttgacc gcgcgcgtcg tcgaccgcct
     2161 gcgagcccgg ctgcgcggcg cgcgcccggc acccggctgc cgcggactgt tgctggtgct
     2221 gaccggcgtc agcgagactg ccactgcgcg cggtagctca gaccgagctg gatgatccgg
     2281 tcgagtatgt cgtcgtaggc gattcccgcg gtctccgccg actcggcgaa ttcgccgcca
     2341 taggacagat cggggttggg attggcctcg agcacgtaga ttcggccgtc gtcggtcacg
     2401 cgcaggtcga tgcgggcgta gccggtcagg ttgagcaccc ggtaggcccg cttgcacgtg
     2461 cgccggatcc gctcgcccag cgcctcgtcg aggtcgtcgg ccgggccata gacgatgccc
     2521 gatcgctgct ggtacgccca gtcccacttg atcttcgccg tagcgatcag cggcgcggcc
     2581 cggcgcgggt cggcgtagcg cagctcccac accggcagcg tgtcgagacg gcggttgccc
     2641 cagacgccga cgtacagctc gcgcccgtcg atgtaccgct cggcgatcgc gtcggttccg
     2701 gtctggcggt ggacgaactc ggtgcgctcg cgcagttgct tggcgtcgtg aaccagcgac
     2761 gcctgcgcga tgccgaccga gccttcagcg gtcaacgact tgacgagcag cgggaaagcg
     2821 agacgcttgg gcggctcgcg gaagctgcgg ttcatcgggt agacggcgaa gtccggaacc
     2881 gagatgcggt ggaacgccat gacctgcttg gacaacgcct tgtcgtgcgc cagcatcagg
     2941 ccgcgcggat tgcatcccgt gtagtggcgc ttcatcagct cgaggtagct gacgacgtgc
     3001 tggtcgtaga tcgccacgcc gtggaactcc tcgagcaggt tgaaggtgat ctgcggattg
     3061 aactgctcca tcgcctggcg cagcacgccg agatcggtgt cgacgccgag cggcagcacc
     3121 tcgtgaccca tctcgcgcaa ggtcgaggtg acgtcgaact cggtcttgaa cggggcgagc
     3181 tccttgtccg acagaccctc gatcgactcg ggcgggacga ggctctcgtg catcaacacc
     3241 agcacgcgtc gtcgggcggg tcccgttcgg cgggatcgcc gtcgcggccg cgcctcgtcg
     3301 gtcacagcgg aatctcgtgc ttgccgacgt gcacgcgatg catcgtgtgc ttggtcagca
     3361 gcagcagggc ccccgatcga gcctcgtggg ccggcacggc gacgcgcagc ttgagctcgc
     3421 ggcagcgctc gatcatctct tgcagcacct gatcgatcgt gtaggcgtgc gcgccggtcc
     3481 actcggagac cgtctcgcgg atcttcttgc gcgagcttcg cagaaagctg gacgccgtcg
     3541 gtcgcgcggc gtagtgccgg tcgtccgaga acagccgccg caggtcgcgg tcgtagaaat
     3601 ccgaccactg aacgccgtag cgttcccgtt tcttgtcgta gtactcgccg agcgtctgct
     3661 tcagctgcga gaccggctcg acccgttcgc gccggagaac gaccggcggc ttcccggcga
     3721 tctcgtccat cgtccgatcg acgtactcga gcttgcgcag cgcggccggc cagtcgcggt
     3781 agtggctgcg ccagtgcgag cggggcttga gccaaacggc gaaggtctcg gcgaaatcct
     3841 ccgccggatg ggcctgtgcg taccaggcgt ccaggtgcag cacgtaattg cggctgttgg
     3901 gcttcgggcg atagtagtcg ggatagggct cgctgtagcg gccgaacgtc tggcgccagc
     3961 ccttcctgaa atgcagacga taggcactgt cgatcgcgtg tcccgcctcg tggcgcagca
     4021 cgcgcatgca cgagcggtcg ttgccgccct cgacctcgag catctgcttg gcctcgagcg
     4081 cgacgagccg gcggtgggcc aggtagaacg ggatcgcgat tccgggaacg ccgtcggggg
     4141 agaaccattc gctcgacagc cagaccgccg gccggaagca caggccgcgt tcgtccagct
     4201 cggcatagag ccgcttgacc agccgctcga gctgggttcc gtcgaccttc aagcccaggt
     4261 cgcaaatacg ggtcgcgagc aaccgctcat cgtcccagcg ggcccaggcg cggcgcgtcg
     4321 gttttctcgt tctgcccctg gcggtcaccg tgtgttccgt cctcgacggg cagaccctac
     4381 cagccctgcg ccggccggac aagatcgcgg ggtaccgccg gcgtcagacg ccgagcacgg
     4441 tcgccgccgc ctcgtagtcc tcgggacgga cgtagagcac gtaaccgtag tgtccgtgac
     4501 cgtcggtcac gccgtcggac gcatagacgt tgacccgcgc gtcggtcagc cgctggtgcc
     4561 agtcgacgag cgcgcccatt cggtcatcgc cctggatcag gaacgcgtcg tgcggtccgg
     4621 tcagcacgac gccgttctgt tcggccgcgc gagcgagctg atcgacgcgg tcggggaaca
     4681 gcacgagctg ggtgacaccg ggccccatcg gaatcgcgcg aaacgcgagc aggttgacct
     4741 cggccgatgc gacctgcgtc agcagccgat aggcggcgcc gggctcgtcc ttgaccgtgg
     4801 catagaagta ctggacccgg cggaccttgt tccccatgat gcacctccgg ggttgttcgc
     4861 ctctggcacc attatggtcc agccgcggcg ccccgggagg cgcgaatcag gaacagccgc
     4921 tcgtggcgcc gcagttgacg caggcgtagc acgccccgtt gcggatcatg atctctccgc
     4981 aatcgtggca cggcggggca tcggcctgcg cgacgtaaac cgctcgctcc cggtcgcgta
     5041 gctcgtcggg tgcggcggcc tgcgggtcgg gttcgatttc cgagccgttg accggctcgg
     5101 atgccttcac cggttggtcc tccgccggca ggaactgcag cgccagccag cggaagacgt
     5161 agtcgatgat cgacttcgcg atcggaatcc gcgaattgcc ggtgaagccg gacggctcga
     5221 accgcacgtg ggcgaacttg tcgaccagaa aacgcagctc gacgccgtgc tgcagggcga
     5281 acgagatggc ggtcgcgaag gcgtcggcga agccggccag ggtcgagccg gccttgccca
     5341 gcgtgatgaa gatctcgccc ggcttgccgt cttcgtacag gccgacggtg atgtacccct
     5401 cgtgaccggc gacggaaaac ttgtgcgtga ccgactggcg cgtgtcgggc agtttccg
//
LOCUS       DASPGN010000082        13160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1537074, whole genome shotgun sequence.
ACCESSION   DASPGN010000082 DASPGN010000000
VERSION     DASPGN010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 13160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13160
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537074"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="VD788_02290"
     CDS             complement(<1..1636)
                     /locus_tag="VD788_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXV75119.1"
                     /translation="MRTRAQRGAAAGTILLLLAALGATAQEKTDQGYVGFGADSVSPE
                     ALARFAPPPIDPQLRARVERTMDIRSPGAGRLAPDGETLFFTWGVTGTTQIWRVDEPK
                     GFPVQMTGGEDSSRLLDITPDGRYLIISRDSSGDEQFGLYLQPTEGGELRTIFKGHKV
                     RAGYEFVTDDSRSVYYRANDVRPDSYTIYRYDVEQGERETVFDREGLWVVADFEPDRG
                     FLLSRLKGNLYADHFLWKEGSDALQPILGQDEMVEYGVAFAANDDEYVVHTNKFGEFR
                     RLYRYRGGEFTPLSPELAHDVADFSIDDARKRIYYSVNRNGYYGAAVIDATTLEPVEM
                     PALPEAEQVYFGSTTRDGRATTISLETARAPAAAYVYDWKTARLTQWVVPSTPEVDTS
                     RFVRAELDGYPARDGTEVPMFVWRSDDCRKRACPVIVNFHGGPESQARPGFSPTTQLF
                     VDAGFVYVRPNVRGSDGYGKAWLDADNGSARLDVLSDIEDCALHIRKAWARDGEAPKI
                     GIYGGSYGGYSTLVGMTIYAGAYDAGVAVVGMSNLLT"
     gene            complement(1697..2092)
                     /locus_tag="VD788_02295"
     CDS             complement(1697..2092)
                     /locus_tag="VD788_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75120.1"
                     /translation="MMESNGLLVLLAGLAILATWAWRLIHRATRLEFGERGILDRNLG
                     LGWIRWDEIEGAYQRHALEQTSVFLRLRPTERILRRLGQRREAGPGRAGRSFDVRVDL
                     TDADLTAVELVQEIMARGREAAPAPDRHS"
     gene            complement(2232..3686)
                     /locus_tag="VD788_02300"
     CDS             complement(2232..3686)
                     /locus_tag="VD788_02300"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXV75121.1"
                     /translation="MDVRAEIVRIPTLSLAIAVATACVVNPATGKRQFSLVSESDEIQ
                     IGRETDRVIVEQMGLYGGDELQGYVRQVGERLAAVSERPQLDWTFRVVDDPQVNAFAL
                     PGGYIYITRGILAYLQSEAELATVVGHEIGHVTARHGADQMTKAQLTQAGLVLGTALG
                     PDWARGLSDYAQIGAGLMMMKFGRDDERQADELGLRYMLAADYDARPAAGVFDMLDRV
                     SAASGGGGTPAWLSTHPAPANRSQNMQAAVVSLERDFSDSKVGRQEYLVHIDGIVFGS
                     DPREGYFTDQRFIHPQLRFRIDFPSGWTTSNTRAAVSGTSPGNDAVVAISHAAGSSRE
                     RALESFIAQEGIAGGSYWRDAVGGVPVSARGFSATSGQGAVRGIVMFVERESTVLRVI
                     GYSLDPAWGANRAAIEGALASYAVVTDPAALGVEPARVRVVDAPSSGSLGQFARQTGA
                     TASVEQLALLNRMPVDGSVVAGRPYKTVTGGPAR"
     gene            complement(3704..5449)
                     /locus_tag="VD788_02305"
     CDS             complement(3704..5449)
                     /locus_tag="VD788_02305"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXV75122.1"
                     /translation="MNGTTDRPGWPVARCLLLTIPWLVAGCADDARPVETADLILTNG
                     RLVTVDDARPEAEALAVRGGLILAVGSNDEIARFRGDATEVIDLAGRLAIPGFIEGHA
                     HFTGLGQSLMIVDLRDAHGWDEVVARVAAAADEAPPGEWILGRGWHQEKWDSAPRPDV
                     EGYPVHDRLSAATPDHPVALTHASGHAAIVNALALEAAGISDDTADPPGGTILRDADG
                     RATGVLRETAEGLVERAIEARDRERSPQAIEERTRRQIELATAECLSNGVTSFQDAGS
                     SFSTVDRLAAAAEDGTLGVRLWVMLNESNAALAQVTERFPLIDEHDQMLTVRAVKRVM
                     DGALGSHGAWLLEPYSDLGDSVGLPTIAVEELAQAARIALDRGLQLCVHAIGDRANRE
                     VLDVFETAYAEAPDADLRWRVEHAQHLHPDDLPRFADLGVIASMQAVHCTSDGPWVPD
                     RIGTARAATGAYMWRALLDSGAVVTNGTDAPVEEIDPIANFHASVTRRMRDGERFFPE
                     QRMTREQALRSYTLDAAYAAFEEGLKGSLTPGKLADVTVLSRDIMTVPEEQILDAEVV
                     YTIVGGRLAYRADGS"
     gene            complement(5482..6054)
                     /locus_tag="VD788_02310"
     CDS             complement(5482..6054)
                     /locus_tag="VD788_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75123.1"
                     /translation="MTQPIRYSRVAGACLLLALAVGPISAGVAVEIDSATLNEILGAV
                     SVNRVEVALTENNTVVVELHDLHVIGLVPSTRDGQRHAILTSVLVVAPELGLKLPLEP
                     RVALDVVQLDGASMLELRFEDLGLRVPLMGQVNLAAMLPPMRYPATNVWMLDGARGGV
                     PVASRLSSIEMDQRRVRFLLDVEVVATPEE"
     gene            complement(6191..7648)
                     /gene="nrfD"
                     /locus_tag="VD788_02315"
     CDS             complement(6191..7648)
                     /gene="nrfD"
                     /locus_tag="VD788_02315"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXV75124.1"
                     /translation="MSVIAQHGTRSLDNTTDDPTRRAPLVLGGNDFNSVTEKICKLAE
                     ARRPTRAWNIAFGISASFTLLLGAMIAYLFIEGIGVWGVNAPVGWGFAIINFVFWVGI
                     GHAGTLISAILFLFRQRWRTGINRFAEAMTIFAVICAGIFPGIHVGRVWFAYWLFPYP
                     NQMLMWPNFRSPLLWDVFAVGTYFTVSLLFWYMGMIPDLATLRDRATSRIRQLVYGLL
                     SMGWRGSNRHWQNYERVYLLLAALATPLVLSVHSVVSFDFAVSVLPGWHTTILPPYFV
                     AGAIFCGFAMVVLLAVPSRRFFGLEDVITLRHLENMNKIILATGMMVGYAYATEFFIA
                     WYSGNVYERFTFVNRLLGPYWWSYWIMWSCNVIAPQLFWSKRIRTTPWAMMIVVIFVN
                     IGMWFERFVIVVTSLSRDYLPSSWGVYVPSWVDIATLAGSFGLFFTLFLLFVRFVPMV
                     AMAEVKAVMPQADPHPHAAAGPAAIGPRLEPSERR"
     gene            complement(7946..8491)
                     /locus_tag="VD788_02320"
     CDS             complement(7946..8491)
                     /locus_tag="VD788_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75125.1"
                     /translation="MRVGFLETSACLAVLLSSSLAAAQWQRNGKQWPDSAWSKSESGF
                     SAMLLMSDNPDEVVRDWETPDAPVTVRAVNTITRGVPIVGFVVFAGCEPDERGNCNAS
                     VDFIVLKPDGSEYVNFPDRDLWRGKPAPPKDTLRLAAEYVGVIIEPDDPLGEYEVRAS
                     IHDRNADAHLELRRTFTATAR"
     gene            complement(9026..11074)
                     /locus_tag="VD788_02325"
     CDS             complement(9026..11074)
                     /locus_tag="VD788_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75126.1"
                     /translation="MSHGRITLGLVIRVAVVLTARAGSILAQADNICDPGEAPDIIVG
                     EILGSRSWGTVDGISAYSFGTDSCNIGTCQANWFALSPLHPVIAQNLFRLKDGRLEQI
                     GQSWVKHGFGTIDDKTCSPDCLEAPADGFLGVNCSDVYFSGLNGTQQGMGPKSEVRAW
                     NGVFPWPPTDVDLTGDAIYKRLQVHAEDLDPALNAGAQYFVEAQYVTRDDATGGNQNN
                     NASHRPVDVTIASDGTFELQVSGGTAREIPAIFAWQSADPEVWISALELTGRYYVGSR
                     ATDLGDGTWRYEYAVHNLNADGAQGFVVPTSGDAAVSNIGFHDVDYHSGEPWDGTDWP
                     GTFDPQAGVVSWAVIAQDDFANVLRWGTLYNFRFDCDRPPVAGMVELTPFVNDARAVT
                     AGPGSVDAWLPQACDDDGVCEPAEDCNNCPGDCTGMPISFCGDGICDPASGEDCESCS
                     RDCNGQRGNPAGRFCCGDGDGQNPVACSDPRCTTAGFACSTPCCGDGTCNFGEGRCSC
                     APDCGFPAHVELVCGDGSDDDCDAATDCDDLDCCLDVSCADGIDADGDSVAECDCDDG
                     NATAWSMPGEARDLTLAHDTPSGVATLSWTAPVDGGASAVSYAVLRSGDESDFVAAAV
                     CLSAADPLATVATDVAAPPSGGVFHYLVRAENACPAGAGLGDLGSSSAGAFRDGRACP
                     "
     gene            complement(11153..12349)
                     /locus_tag="VD788_02330"
     CDS             complement(11153..12349)
                     /locus_tag="VD788_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXV75127.1"
                     /translation="MKGETQTVVIGAGPVGKFAALSLARLGVEVEILDEGSRGAVHSY
                     ALALHPESLRLLDELGVADELVASGQRIERMGFHRRDERLGQLDFSELGGPFPFVLVA
                     PQSALEQALEQRLNREKVRVWWKHQALAVSQDAARVALRVGRREKYSMGYPVAGTEWM
                     ITRESDVHSRFLIGADGYHSFVRRSHDDAFESGGPAQVFSVYEFPLTVDAPQEARVFF
                     HDGTTNVFWPLHDRRGRLSFQVEPGSPPSPSVEGLNDLIRERVSWLPAEIEQVYWTST
                     AMFERRLAKSLGSNRIWLAGDAAHLTGPVGAQSMNVGLREAHDLARRIADGIARGASA
                     DAIGSYDADWRAEWRALLNLDGRLEATPALAKALGAQLAELPACVPASGADLDALLSQ
                     IGVRLR"
     gene            complement(12339..12821)
                     /locus_tag="VD788_02335"
     CDS             complement(12339..12821)
                     /locus_tag="VD788_02335"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXV75128.1"
                     /translation="MVRWVERWVRWADSRRHLYFDFVRIYMGVALFVKGLQFVSDRTY
                     LEGVLTEEMRFVPTALTHYIPAAHLSGGFLLTIGLLTRFAAAIQIPVLFGATFVVYMP
                     QGFFTYGQNFEFAALVLFLLVMVFLYGGGRLSADYYLFGPGRLDGRVSDPGDDHAIEG
                     "
BASE COUNT         2149 a         4579 c         4426 g         2006 t
ORIGIN      
        1 aggtcagtag gttgctcatg ccgacgaccg ccacgcccgc gtcgtaggcg ccggcgtaga
       61 tcgtcatgcc gacgagcgtg gagtagccgc cgtagctgcc gccgtagatg ccgatcttcg
      121 gcgcctcgcc gtcacgcgcc cacgccttgc ggatgtgaag cgcgcagtcc tcgatgtcgc
      181 tgagcacgtc gagccgagcg gacccgttgt cagcgtcgag ccacgccttg ccgtagccgt
      241 cgctgccgcg gacgttcggc cgcacgtaga cgaagccggc gtcgacgaac agctgcgtgg
      301 tcgggctgaa gccgggccgc gcctgcgact ccgggccgcc gtggaagttg acgatcaccg
      361 gacacgcccg cttccggcag tcgtccgagc gccagacgaa catcggcacc tcggtgccgt
      421 cgcgcgccgg gtagccgtcg agctcggcgc gcacgaaccg gctggtgtcg acctcgggcg
      481 tgctcggtac gacccactgc gtcaacctcg ccgtcttcca gtcgtagacg taggccgccg
      541 ccggtgcccg cgcggtctcg agcgagatcg tcgtcgcgcg cccgtctcgc gtcgtcgagc
      601 cgaaatagac ctgctcggcc tccggcagcg ccggcatctc gaccggctcg agcgtcgtcg
      661 cgtcgatcac cgccgcgcca tagtagccgt tgcggttgac gctgtagtag atccgcttgc
      721 gtgcgtcgtc gatcgagaag tcggcgacgt cgtgcgccaa ttcgggcgac agcggcgtga
      781 actcgccgcc gcggtagcgg tacaggcggc ggaactcgcc gaacttgttc gtgtgcacca
      841 cgtactcgtc gtcgttcgcg gcgaaagcca caccgtactc gaccatctcg tcctgaccga
      901 ggatcggctg gagcgcgtcc gatccctcct tccacaggaa gtggtcggca tacaggttgc
      961 ccttgagccg cgagagcaga aagccccggt cgggctcgaa atcggcgacg acccacagtc
     1021 cctcgcgatc gaacaccgtc tcgcgctcgc cctgctcgac gtcgtagcgg tagatcgtgt
     1081 agctgtcggg gcggacgtcg ttggcgcgat agtagaccga gcgcgaatcg tcggtgacga
     1141 actcgtagcc ggcgcgcacc ttgtgcccct tgaagatcgt gcggagctcg ccgccctcgg
     1201 tcggctgcag atagaggccg aactgctcgt cgccgctcga gtcgcgcgag atgatcaggt
     1261 agcggccgtc gggagtgatg tcgagcagtc gcgaggaatc ctcgccgccg gtcatctgga
     1321 ccgggaatcc cttcggctcg tcgacgcgcc agatctgggt cgtgccggtc acaccccagg
     1381 tgaagaacaa cgtctcgcca tccggcgcga gccgccccgc gcccggcgag cggatgtcca
     1441 tcgtgcgctc gacgcgtgca cgtagctgcg ggtcgatcgg cggcggagcg aagcgtgcca
     1501 gcgcttccgg gctgacgctg tcggcgccga agccgacgta tccctggtcc gtcttctcct
     1561 gggcggtcgc ccccaaggcg gcgagtagca gcaggatcgt gccggccgcc gcaccccgtt
     1621 gcgcgcgagt tcgcatctcc gtacccccgt gcccgttccg cagacgacgg cattgtctca
     1681 cgggtcgacg ggtgtgctag gagtgccggt ccggcgcggg ggccgcctcc cgtccgcggg
     1741 ccatgatctc ctggaccagc tcgaccgcgg tcaggtcggc atcggtcaga tcgacccgta
     1801 cgtcgaacga cctcccggcc cggcccggtc ccgcctcgcg gcgctgcccg agccggcgca
     1861 gaatgcgctc ggtcggtcgc aaacgcagga aaacgctggt ctgctccagc gcgtggcgct
     1921 ggtaggcgcc ttcgatctcg tcccaacgga tccagccgag ccccaggttg cggtcgagga
     1981 tgccgcgctc cccgaactcc agccgggtcg cgcggtggat cagccgccag gcccaggtgg
     2041 cgaggatcgc gagcccggcg agcaggacga gcaacccgtt cgattccatc atcgcgctcc
     2101 ggaggcgccg cggtcggcgc ctggtgctac tcctccatac gcgcgccgcg ggccgtccgt
     2161 tgagtccgga gccgatcggc tgcggattat cgtccgcagc attggtaaca cgatgtgagt
     2221 tcgacgtcgg ttcagcgggc cggcccgccg gtgaccgtct tgtacggccg tccggcgacg
     2281 acggagccgt cgaccggcat ccggttgagc agggccagct gctcgacgga agcggtcgcc
     2341 ccggtctgcc gcgcgaactg gccgagggag ccgctcgacg gcgcgtcgac gacgcgcaca
     2401 cgggccggct cgacccccag cgcggccgga tcggtaacga ccgcgtagct cgcgagcgcg
     2461 ccctcgatcg ccgcccggtt cgcgccccac gccgggtcga gcgagtagcc gatgacgcgc
     2521 agcacggtcg attctcgctc gacgaacatc acgatgcccc tcaccgcccc ctgtccgctc
     2581 gtcgcgctga agccgcgcgc ggagaccggg accccgccga ccgcgtcccg ccagtacgac
     2641 ccgccggcga tcccttcctg ggcgatgaac gactcgagcg cgcgctcgcg cgacgagccg
     2701 gccgcgtggg agatcgcgac gaccgcgtcg ttgccgggac tcgttcccga aaccgcggct
     2761 cgcgtgttcg aggtggtcca gccggaagga aagtcgatgc ggaaccgcag ctgcgggtgg
     2821 atgaaccgct ggtcggtgaa gtagccctcg cggggatcgc tgccgaagac gatgccgtcg
     2881 atgtgaacca ggtactcctg ccgtccgacc ttggagtcgg agaaatcccg ttcgagcgag
     2941 acgacggcgg cctgcatgtt ctggctgcgg ttggccggcg ccgggtgtgt cgacagccac
     3001 gccggcgtgc ccccgccccc gctcgcggcg ctgacgcggt cgagcatgtc gaacaccccc
     3061 gccgccggtc gcgcgtcgta gtccgcggcc agcatgtacc gcagaccgag ctcgtcggcc
     3121 tgccgctcgt cgtcgcgccc gaacttcatc atcatcagtc cggcaccgat ctgcgcatag
     3181 tcgctcagac cgcgcgccca gtccgggccg agcgcggtgc cgagcaccag cccggcctgg
     3241 gtcagctgcg ccttggtcat ctggtcggcg ccgtgccggg cggtgacgtg gccgatctcg
     3301 tgacccacca cggtcgccag ctcggcctcg ctctgcaggt aggccaggat gcctcgcgtg
     3361 atgtagatgt agccgccggg gagcgcgaac gcgttgacct gcggatcgtc gaccacccga
     3421 aaggtccagt cgagctgtgg ccgttcggag accgccgcca gacgctcgcc gacctggcgc
     3481 acgtaaccct gcagctcgtc gccgccgtac agacccatct gctcgacgat gacccggtcg
     3541 gtctcgcggc cgatctggat ctcgtcggat tccgacacca acgagaactg gcgtttcccc
     3601 gtcgccggat tgacgacgca cgccgtcgcg acggcgatgg ccagcgagag ggtcgggatc
     3661 cggacaatct ccgcgcgcac gtccatcggc ggctctcctc gggtcagctt ccgtcggcgc
     3721 gatacgccag cctgccaccg acgatggtgt agaccacctc cgcgtcgagg atctgttcct
     3781 cgggcaccgt catgatgtcg cgtgacaaga cggtgacgtc ggccagtttg cccggcgtca
     3841 acgatccctt cagcccttcc tcgaacgccg catacgccgc gtcgagcgtg taggagcgca
     3901 gcgcctgctc gcgcgtcatc cgttgttcgg ggaagaagcg ctcgccgtcc cgcatccggc
     3961 gggtgacgct ggcgtggaag ttggcgatcg gatcgatctc ctcgaccggc gcatcggtcc
     4021 cgttcgtgac cacggcgccc gagtcgagca acgcacgcca catgtaggcg cccgtcgccg
     4081 cgcgcgcggt gccgatccga tccgggaccc acgggccatc ggacgtgcag tgcaccgcct
     4141 gcatcgacgc gatcacaccg aggtcggcga accgcggcag gtcgtcgggg tgcaggtgct
     4201 gcgcgtgctc gacccgccag cgaaggtcgg cgtcgggggc ttcggcgtac gcggtctcga
     4261 atacgtcgag cacctcgcgg ttggcacggt cgccgatggc gtgcacgcag agctgcaggc
     4321 cccggtcgag cgcgatgcgg gccgcttgcg cgagctcctc gacggcaatc gtcggaagac
     4381 cgaccgagtc gcccaggtcg ctgtacggct cgagcaacca cgcgccatgc gacccgagcg
     4441 cgccgtccat cacgcgtttg acggcgcgca cggtcaacat ctggtcgtgc tcgtcgatca
     4501 gcgggaaccg ctcggtgacc tgcgccagcg cggcgttcga ttcgttgagc atcacccaca
     4561 gacggacacc gagcgtgccg tcctcggcgg cagccgccaa acggtcgacg gtcgagaacg
     4621 acgaaccggc atcctggaag ctggtcacgc cgttggacag acactcggcg gtggccagct
     4681 cgatttgacg ccgcgtccgc tcctcgatcg cctgcggcga ccgttcccgg tcgcgcgcct
     4741 cgatcgcgcg ctcgaccaac ccctcggccg tctcgcgcag cacgccggtc gcccgtccgt
     4801 cggcgtcacg caggatggtg ccgcccggcg gatccgcggt gtcgtcgctg atgccggcgg
     4861 cctcgagcgc gagcgcgttg acgatcgccg catgaccgct ggcgtgcgtc aacgccaccg
     4921 ggtggtccgg cgtcgccgcg ctcaaccggt cgtggaccgg gtagccctcg acgtcgggtc
     4981 gtggagcaga gtcccatttc tcctgatgcc agccgcgccc gaggatccac tcgcccggcg
     5041 gagcctcgtc ggccgccgcg gccacccgcg cgaccacctc gtcccagccg tgcgcgtcgc
     5101 gaaggtcgac gatcatcaac gactggccca gaccggtgaa gtgggcgtga ccctcgatga
     5161 agccgggtat cgccaaacga ccggcgaggt cgatcacctc ggtcgcatcc ccacgaaacc
     5221 gggcgatctc gtcgttgctg ccgaccgcga ggatcaagcc gccgcgaacc gccagcgcct
     5281 cggcctcggg gcgggcgtcg tcgaccgtga cgagccgacc gttggtcagg atcagatcgg
     5341 cggtctcgac ggggcgcgca tcgtcggcgc agccggcgac gagccacggg atcgtcaaca
     5401 acaaacagcg ggcgaccggc cagcccggcc gatcggtcgt tccgttcatc gttactcctc
     5461 gcgatcggga ttatatcgag gtcattcctc cggggtcgcg acgacctcga cgtcgagcag
     5521 gaagcggacc cgccgctggt ccatctcgat cgatgacagc cggctcgcga cgggaacgcc
     5581 gccacgcgcg ccgtcgagca tccacacgtt ggtcgccggg tagcgcatcg gcggcagcat
     5641 cgcggccagg ttgacctggc ccatgagcgg gacgcgcagg cccaggtcct cgaaccgcag
     5701 ctcgagcatg ctcgccccgt cgagctgcac cacgtcgagc gccacccgcg gctcgagtgg
     5761 cagcttcagg cccagctcgg gcgcgaccac gaggaccgag gtgagaatcg catgacgctg
     5821 accgtcgcgt gtcgacggca ccaggccgat cacatggaga tcgtgcagct cgacgacgac
     5881 cgtgttgttc tcggtcagcg caacctcgac ccgattgacc gagacggctc ccaggatctc
     5941 gttgagcgtg gccgagtcga tctcgacggc cacgccggcc gagatcgggc cgacggcgag
     6001 cgccagcagc aggcacgcgc cggcgactcg cgagtagcgg atcggctggg tcatgtcacc
     6061 tccaacggcg agagagtgaa cagtgtagcg cgccgcggac gaggtcgacc gcgcgcacac
     6121 ggcggagcca cccgcgagcg gtcggcggaa cgcgacgtaa agggtgcggg attcgacgaa
     6181 acggcgtcga ctagcggcgc tcggacggtt cgagccgggg gccgatggcc gccggccccg
     6241 ccgccgcgtg cggatgcggg tcggcctgcg gcatcaccgc cttgacctcc gccatcgcga
     6301 ccatcggcac gaaacgaacg aacaacagga acagcgtgaa gaacagaccg aagctgccgg
     6361 ccagcgtggc gatgtcgacc caggacggga catagacacc ccaactcgac gggaggtagt
     6421 cgcggctgag cgacgtgacg acgatgacga accgctcgaa ccacatgccg atgttgacga
     6481 agatcacgac gatcatcatc gcccacggcg tcgtgcggat ccgcttcgac cagaacagct
     6541 gaggcgcgat gacgttgcag ctccacatga tccagtaaga ccaccagtag ggtccgagca
     6601 gacggttgac gaaggtgaag cgctcgtaga cgttcccgct gtaccaggcg atgaagaact
     6661 cggtggcgta ggcgtagccg accatcatcc cggtggccag gatgatcttg ttcatgttct
     6721 cgaggtgtcg cagcgtgatc acgtcttcca gcccgaagaa ccgccgcgac ggaacggcca
     6781 gcagtaccac catcgcgaat ccgcagaaaa tcgcgccggc gacgaagtac ggggggagga
     6841 tcgtcgtatg ccaaccggga agcacggaca cggcgaagtc gaagctgacc accgagtgga
     6901 cgctgagcac gagaggggtg gccagcgcgg ccagcagcag gtagacgcgc tcgtagttct
     6961 gccagtgccg gttcgagccg cgccagccca tcgacagcag cccgtagacc agctggcgaa
     7021 tgcggcttgt ggcccgatcg cgcaacgtgg ccaggtccgg gatcatgccc atgtaccaga
     7081 acagcagcga gacggtgaag taggtgccga ccgcgaacac gtcccacagc agcgggctgc
     7141 ggaaattggg ccacatcagc atctggttgg ggtaggggaa cagccagtag gcgaaccaca
     7201 cgcggccgac gtgtatcccg gggaagattc cggcgcagat caccgcgaag atcgtcatcg
     7261 cctcggcgaa gcggttgatt cccgttctcc agcgctgacg gaacaggaac aggatcgccg
     7321 aaatcaacgt tccggcgtgt ccgatgccga cccagaagac gaagttgatg atcgcgaagc
     7381 cccaaccgac cggggcgttg accccccaga cgccgatccc ctcgataaag aggtaggcga
     7441 tcatcgctcc gaggagcagt gtgaacgacg ccgaaatgcc gaacgcgata ttccacgcgc
     7501 gcgtgggtct ccgcgcctcg gcgagcttgc agatcttctc cgtcacggag ttgaagtcgt
     7561 tgccgccgag gaccagcggg gcacgtcgag tcggatcgtc ggtcgtgttg tccagcgagc
     7621 gtgtgccgtg ctgtgcgatg acactcatcg atcgccgatc tccggagctc gatcgcgcat
     7681 gacggcgcgc cgctggccgc cttcgcgcgg tccgcacgct gtctcgagca acgcacgcga
     7741 ttggactcgc catcatagcg atgcgtcagg gtggccgcaa ggtgatggcc gccgctccaa
     7801 cgctcgtcga gaagccgtac ccgggtgatc ctcagcgtat acgaagaagc cccgacgggc
     7861 atggccggtt ccggagcgtg ccgagccgca cgaatccggc gagattcgcg tgggtgcagg
     7921 tggcggcgtc gcaggcgccg gagccctacc gagcagttgc cgtgaaggtc cgcctcaact
     7981 cgagatgggc gtcggcgttg cggtcgtgaa tcgaggcccg cacctcgtac tcgccgagcg
     8041 gatcgtcggg ctcgatgatc actccgacat actcggcggc gaggcgcagc gtgtccttcg
     8101 gcggggccgg cttgcctctc cacaggtcgc gatccgggaa gttgacgtac tcgctgccgt
     8161 cgggtttcag cacgatgaag tcgaccgagg cgttgcagtt gccccgttcg tccggttcgc
     8221 aacccgcgaa gacgacgaaa cccacgatgg gcacaccgcg ggtgatcgtg ttgaccgccc
     8281 ggaccgtgac gggcgcatcc ggggtctccc agtcgcggac gacctcatcg gggttgtccg
     8341 acatcaacag catcgcggag aacccgctct ccgacttcga ccacgcggaa tccggccact
     8401 gctttccgtt ccgctgccac tgcgctgccg caaggctgct cgacagcagc accgccaggc
     8461 aagccgaggt ttcgaggaat cctacgcgca tgcctcaccg gtacgaccgt tccggaggtt
     8521 tcgctaccga caagatcgat tccgccgtgg gcgccggggc actcgccttg acccggctgc
     8581 acctccccga cggacgctcc acgggtttgc tggacgtcag ggacgacccg tccgcagcgc
     8641 acgagtggat ctcgcggttc ccgtcgcgtt ccgatacaaa ggcgatcctc cttccatcgg
     8701 gagcccatgc ttcgagaagt gccggtggta ctcgccgcag acgatgtatt tgtatccgga
     8761 aggcagcgtc gccgatgcgc cgttgggagc gagcgcttcg acgccgccga agcgtccctc
     8821 gtccacggtc gcactcccga ggttgtgcgc cagacgtctg cgcaaccgcg gcgttcggca
     8881 ccatggcgac cacggcgcgc agcggcgagg attggattcc gggattgcgc tgagccgacc
     8941 gtagccgtgc tccgagcgcc ggctgcggtg tcgcggacgg ggcgacgggg ctgccgcgat
     9001 tcgcggcagc cggtcggccg cgcgatcacg ggcaggctcg tccgtctcga aacgctccgg
     9061 cggagctcga cccgaggtcg ccgagccccg cgccggccgg gcaggcattc tccgcgcgca
     9121 cgagatagtg gaacactccg ccgctcggcg gcgcagcgac gtccgtcgcg acggtggcga
     9181 gcggatcggc cgcggacagg cacaccgcgg cggcgacgaa gtccgattcg tcccccgagc
     9241 gtaggaccgc gtaactcacc gccgacgcac cgccatcgac cggtgcggtc caggacagcg
     9301 tcgccacacc gctcggggtg tcgtgggcca gcgtgaggtc gcgggcctct ccgggcatcg
     9361 accacgcggt cgcgttcccg tcgtcgcagt cgcattcggc gacggagtcc ccgtcggcgt
     9421 cgattccgtc ggcgcacgac acgtcgaggc agcagtcgag atcgtcgcag tccgtcgcgg
     9481 cgtcgcagtc gtcgtcgctg ccgtcgccgc acacgagctc gacgtgggcc ggaaacccgc
     9541 aatccggcgc gcagctgcac cggccctcgc cgaagttgca cgtgccgtcc ccgcagcacg
     9601 gcgtcgagca tgcgaaaccg gccgtggtgc agcgggggtc cgagcaagcg accgggttct
     9661 gcccgtcccc gtccccgcag cagaaacgcc cagcggggtt gccgcgctgg ccgttgcagt
     9721 cccggctgca cgattcgcag tcttcgccac tcgccgggtc gcagatgccg tccccgcaga
     9781 agctgatcgg catccccgtg cagtcgcccg ggcagttgtt gcagtcctcc gccggctcgc
     9841 acaccccgtc gtcgtcgcag gcctgcggca gccacgcatc gaccgagccg ggacccgccg
     9901 tgacggcgcg ggcgtcgttc acgaacggcg tcagctcgac catgcccgcc acgggaggtc
     9961 ggtcgcagtc gaagcggaag ttgtacagcg tgccccagcg caagacgttg gcgaagtcgt
    10021 cttgagcgat gactgcccag ctcacgacgc cggcctgcgg gtcgaacgtg cccggccagt
    10081 cggtgccgtc ccacggctcg ccgctgtggt aatccacgtc gtggaacccg atgttcgaca
    10141 ccgcggcgtc gccgctcgtg ggaaccacga acccctgggc gccgtcggcg ttgagattgt
    10201 gcaccgcgta ctcgtaacgc caggtgccgt cgcccagatc ggttgcacgg gagccgacgt
    10261 agtagcgccc cgtcagctcc aacgcgctga tccacacctc tggatccgcg gactgccagg
    10321 cgaagatcgc cgggatctcc cgcgcggtcc caccgctgac ttgcagctcg aacgtgccgt
    10381 cggaggcgat cgtgacgtcg accggacggt gcgaggcgtt gttgttctgg tttccgcccg
    10441 tcgcatcgtc gcgggtgacg tactgcgcct cgacgaagta ctgcgccccg gcgttcagcg
    10501 ccggatccag gtcttcggcg tgaacctgca gacgcttgta gatcgcgtca ccggtgagat
    10561 cgacgtcggt gggtggccag gggaacaccc cgttccatgc ccggacctcg gacttgggac
    10621 ccattccctg ttgggtcccg ttgaggcccg agaagtacac gtcggaacag ttgacgccca
    10681 gaaacccgtc cgccggcgct tcgaggcagt ccggcgagca cgtcttgtcg tcgatcgtgc
    10741 cgaagccgtg cttgacccag ctctgcccga tctgttcgag ccgcccgtcc ttcaggcgaa
    10801 acaggttctg ggcgatcacc gggtggagcg gggacagcgc gaaccagttg gcctgacacg
    10861 tgccgatgtt gcacgagtcc gtgccgaacg aatacgccga gatgccgtcg accgttcccc
    10921 acgaccgact gccgaggatc tctccgacga tgatgtccgg ggcctcaccg gggtcgcaga
    10981 tgttgtcggc ctgggcgagg atcgatcccg ctcgagcggt caggaccacg gcgacgcgga
    11041 tcaccaggcc cagagtgatt cgtccgtgcg acacctcgag gctcccggtg gctggtggtc
    11101 ggaatgtacg agcagccgtc ggtcgggtca acggtgagcg gttggtcgtg cgctagcgca
    11161 agcgcacgcc gatctgcgag agcagcgcgt ccagatcggc gccggacgcc gggacgcagg
    11221 ccggcagctc ggcgagctgc gcgccgagcg ccttcgccaa cgcaggggtc gcctcgagtc
    11281 ttccgtcgag gttcaacaac gcccgccact cggcccgcca gtcggcgtcg tagctgccga
    11341 tcgcgtccgc cgaggcgccg cgagcgatgc cgtccgcgat ccgccgtgcc aggtcgtgcg
    11401 cctcacgcag accgacgttc atgctctgcg caccgaccgg tcccgtcagg tgggccgcgt
    11461 cacccgcgag ccagatccga ttcgagccca agctcttcgc caggcgacgc tcgaacatcg
    11521 cggtcgaggt ccagtagacc tgctcgatct ccgccggcaa ccatgacacc cgttcgcgga
    11581 tcagatcgtt caggccctcc acgctgggcg acggtggcga gccgggctcg acctggaagc
    11641 tgaggcggcc gcgtcgatcg tgcaatggcc agaagacatt ggtcgttccg tcgtgaaaga
    11701 agacccgcgc ctcttgcggc gcgtcgaccg tcagcggaaa ctcgtagacc gagaagacct
    11761 gagccggtcc gccggactcg aaggcgtcgt cgtggcttcg gcgcacgaac gagtgatagc
    11821 cgtcggctcc gatcaagaac cgcgagtgaa cgtcggattc tcgcgtgatc atccactcgg
    11881 ttcccgccac cggatagccc atcgagtact tctcacgacg accgacgcgt agcgcgaccc
    11941 gcgcggcgtc ctgcgagacg gcgagcgcct ggtgcttcca ccacacgcgg accttctctc
    12001 gattcaaccg ctgctcgagc gcctgctcga gcgcgctctg cggcgcgacg agcacgaacg
    12061 ggaacggacc gccgagctcg gagaagtcga gctgaccgag gcgctcgtct cggcggtgga
    12121 agcccatccg ttcgatgcgc tggcccgagg cgaccaactc gtcggccacc cccagttcgt
    12181 cgagcagccg cagggactcg gggtgcagcg ccagcgcgta gctgtggacc gcgccgcgcg
    12241 acccctcgtc caggatctcg acctcgaccc cgaggcgggc gagcgaaagc gcggcgaatt
    12301 tgccgaccgg ccccgcgccg atcactacgg tctgggtctc acccttcaat cgcatggtcg
    12361 tcccccggat cgctcaccct tccgtcgagc cggcccggac cgaacaggta gtagtctgcg
    12421 gacaaccgtc ccccaccgta aaggaagacc atcaccagca gaaacagcac cagggccgcg
    12481 aactcgaagt tctgtccgta ggtaaagaac ccctgcggca tgtacaccac gaacgtggcg
    12541 ccgaacagca ccggaatctg gatcgcggcc gcgaagcggg tgagcagccc gatcgtcaac
    12601 aggaatccgc cgctgaggtg ggccgccgga atgtagtggg tcagcgccgt cggaacgaag
    12661 cgcatctcct cggtgagaac gccttcgagg taggtccggt ccgagacgaa ctgaagaccc
    12721 ttgacgaaca gcgccacgcc catgtagatc cggacgaagt cgaagtagag atggcgtcgg
    12781 gagtcggccc accggaccca gcgttcgacc cagcgcacca tctcggcctc cccgcgcttc
    12841 gggcgcgcgc ccgtcgaccc atcttcgacg aaccgagcga accgtgtcaa ggatcgcgcc
    12901 gggcgctcgt cgaagacgct tgaccctcga agcgcgggtt tgataggttc agcgacgctg
    12961 tcgactcctg gagagagagc gcggtccgaa tggaaatcag ggacaacgcc cgccacgacc
    13021 cgctgacggg tcgcgcgccg ctaatcgtcg gcggtcatga cttccattcg atcaccgagg
    13081 ccgtggccaa gccgttggag agcaagaccc ccgtcggttg gtggctgttc ttcgttccgt
    13141 ccgttctcgc gctcggctgg
//
LOCUS       DASPGN010000083         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1068767, whole genome shotgun sequence.
ACCESSION   DASPGN010000083 DASPGN010000000
VERSION     DASPGN010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068767"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VD788_02340"
     CDS             complement(<1..298)
                     /locus_tag="VD788_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HXV75129.1"
                     /translation="MPELPDIAVYVEALERLIVGRRLVGVRLGSPFVLRSVAPAPAEC
                     VDRRVRSVVRLGKRVVIGLDGETFLIVHLMIAGRLQWKTRGAKLPGRIGLAAFDF"
     gene            393..1361
                     /locus_tag="VD788_02345"
     CDS             393..1361
                     /locus_tag="VD788_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HXV75130.1"
                     /translation="MNRVELPSIERVREAAARIAPHVHRTPLVRSRFVDATLGAEVSF
                     KCENLQKVGAFKARGATNAVFALSRDAARGGVVTHSSGNHGAALAYAAAKRGIPCVVV
                     MPHDAPATKARAVRGYGAEIIACRRDEREQRCRQIVERRGMTLIHPYENVEVIAGQGT
                     VALEAFEQTGPVDVIIAPVGGGGLIAGTTITAGAVSPATAVWAAEPRAVDDAYRSMLT
                     GEHQPAVADPTSRADGLLTRLGRPAFAAMRQARVRVVVVSESELLQATWDVIQRMKQV
                     IEPSAGTVLAALRVAADEIRGKRVIAILSGGNTDFSWLEDPSFTKD"
     gene            complement(1381..>2323)
                     /locus_tag="VD788_02350"
     CDS             complement(1381..>2323)
                     /locus_tag="VD788_02350"
                     /inference="COORDINATES: protein motif:HMM:NF008024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HXV75131.1"
                     /translation="GPEVLRYEQVELPAPAPGEARIRHTAIGLNFIDTYHRSGLYPLP
                     SLPAVLGREAAGVIEAVGPGVEGFQVGDRVAYPMSAGAYASERNIDAAEIVRLPDAID
                     DRQAAAMMLKGLTAHYLLRRTYRVREGETILVHAAAGGVGSIACQWAKHLGATVIGTV
                     GSEEKAQLARRYGCDHVIIYTAEDFVTRVREITAGNGVPVVYDSVGRATFDRSLDCLS
                     PCGLLVSFGQSSGPVPPFIIGTLASKGSLYLTRPTLATHIAARDDLVRGAEELFRLVS
                     QGAVRIEVRQTFALADAERAHRHLESRRTSGSTVLLP"
BASE COUNT          353 a          839 c          818 g          313 t
ORIGIN      
        1 cgaagtcgaa ggccgcgagc ccgatccggc cgggaagctt ggccccgcgc gtcttccact
       61 gcagccggcc ggcgatcatt agatggacga ttaggaacgt ctcgccgtcg agcccgatca
      121 cgacgcgctt tcccaggcgc acgacggatc gcacgcggcg atcgacgcac tccgcgggcg
      181 ccggcgcgac cgagcgcagc acgaacgggc ttcccagccg aaccccgacg agacgccggc
      241 cgacgatgag gcgctcgagc gcctcgacgt agaccgcgat gtcgggtagt tcgggcacgc
      301 cagccttacc ggatgagcga gcactataac ccgaacgccg ccgaggcgac cgtgcggtac
      361 actcccggga tgcgacgatc ggcgggccgc cgatgaaccg ggtcgagcta ccctcgatcg
      421 aacgggtgcg cgaggccgcg gcgcgcatcg cgccgcacgt ccatcggacg ccgctcgtcc
      481 gctcccgttt cgtcgacgcc acgctcggcg ccgaggtctc gttcaagtgc gagaacttgc
      541 agaaggtcgg cgcgttcaag gcgcgcgggg cgaccaacgc ggtcttcgcg ctgtctcgcg
      601 acgccgcgcg aggcggcgtc gtcacccact cctccggcaa ccacggtgcg gcgctggcct
      661 acgccgcggc gaaacgcggg atcccgtgcg tggtcgtcat gccgcacgac gccccggcga
      721 ccaaggcccg cgccgtgcgc ggctacggcg ccgagatcat cgcgtgccgg cgcgacgagc
      781 gcgagcagcg ctgccggcag atcgtcgagc gtcgcggcat gacgttgatc cacccctacg
      841 agaacgtgga ggtgatcgcc ggacagggaa cggtcgcgct cgaggcgttc gagcagacgg
      901 ggccggtcga cgtgatcatc gcccccgtcg gcggtggtgg cttgatcgcg ggcacgacga
      961 tcaccgccgg cgcggtcagc ccggccaccg ccgtctgggc cgccgagccg cgagcggtcg
     1021 acgatgccta ccgttcgatg ctgaccggcg agcaccaacc ggccgtggcc gacccgacct
     1081 cgcgggccga cggcctgctg acgcggctcg ggcggccggc gttcgcggcg atgcgacagg
     1141 cccgggtgcg cgtcgtcgtc gtttccgagt ccgagctctt gcaggcgacg tgggacgtca
     1201 tccagcgtat gaagcaggtc atcgagccgt cggccgggac ggtactggcg gcgctgcgcg
     1261 tggcggccga cgagatccgc ggcaagcgcg tgatcgccat cctcagcggg ggcaacaccg
     1321 atttctcctg gctcgaggat ccgtcgttca ccaaggactg agccgcgcgc gccccgtgcg
     1381 tcagggcaac agcacggtcg agccactcgt ccgccgcgat tcgagatggc ggtgggcacg
     1441 ctcggcgtcg gccagagcga aggtctgacg cacctcgatc cgcacggcgc cctggctgac
     1501 cagccggaac agctcttccg ccccgcgaac caggtcgtcc ctggccgcga tgtgggtcgc
     1561 gagtgtcggc cgcgtcaggt agagcgaacc cttgctggcc agtgtgccga tgatgaacgg
     1621 cgggaccggc cccgacgatt ggccgaagct gacgagcagc ccgcacggcg acaggcagtc
     1681 gagcgagcgg tcgaaggtcg ctcgaccgac cgagtcgtag accaccggca cgccgttgcc
     1741 cgcggtgatc tcccgcacgc gcgtgacgaa atcctcggcc gtgtagatga tgacgtgatc
     1801 gcagccgtac cgccgcgcca gctgcgcctt ctcctccgaa ccgacggtgc cgatcaccgt
     1861 cgcaccgaga tgcttggccc actggcaggc gatcgagccc accccgcccg cggcggcgtg
     1921 gaccaggatc gtctcgccct cgcgcacgcg ataggttcgc cgcagcaggt agtgcgcggt
     1981 caggcccttg agcatcatcg ccgccgcctg gcggtcgtcg atcgcatcgg gaagccggac
     2041 gatctcggcc gcgtcgatgt tgcgttcgct ggcgtaggcg ccggccgaca tcgggtaggc
     2101 cacgcggtcc ccgacctgga agccctcgac acccggtccg accgcctcga tcacccccgc
     2161 cgcctcgcga cccagcaccg ccggcagcga aggcagcggg tagagcccgc tgcggtggta
     2221 ggtgtcgatg aagttcaggc cgatcgcggt gtgccgtatc cgcgcctcgc cgggcgccgg
     2281 cgccggcagc tcgacctgct cgtagcgcag cacctcggga ccg
//
LOCUS       DASPGN010000084         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1185822, whole genome shotgun sequence.
ACCESSION   DASPGN010000084 DASPGN010000000
VERSION     DASPGN010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1185822"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VD788_02355"
     CDS             <1..863
                     /locus_tag="VD788_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXV75132.1"
                     /translation="KQIKYPLACKDDFGRLRVGAAIGIARDTQDRADALIDANVDVLV
                     VDTAHGHSEGVLTTVARLRERHPEIQLVGGNVATADGAKALIERGVDAVKIGVGPGSI
                     CTTRIVTGAGVPQLSAILAARDTCREADVPLVADGGIKYSGDITKAIAAGASAVMIGS
                     LFAGAEESPGETVLFQGRTFKEYRGMGSIGAMKKGSRDRYFQEDEFDERKLVPEGIEG
                     RVPYKGPLSALVSQLVGGLKAGMGYLGCPDIEALQRNARFIRLTPAGLRESHAHDVII
                     TKEAPNYRLD"
     gene            complement(872..1387)
                     /gene="tsaE"
                     /locus_tag="VD788_02360"
     CDS             complement(872..1387)
                     /gene="tsaE"
                     /locus_tag="VD788_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXV75133.1"
                     /translation="MPSEITPHSRSRETDGGDSSRTVFSLSEDETLELGRTVARKLKG
                     GELILLEGDLGLGKTVFARGVAIGLGLAPEDVSSPSFTLIQEYRGGRLTMFHVDLYRL
                     ASDEEIATLGLEEIQTDRAVVLVEWGEKIPPRYRRDAIVVRLHDVGEDSRRIEISGLE
                     RDRPTERRGDA"
     gene            complement(1404..>2311)
                     /locus_tag="VD788_02365"
     CDS             complement(1404..>2311)
                     /locus_tag="VD788_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXV75134.1"
                     /translation="LEWWDAAAAAPLLGRRAADSHKGTYGHLLVVAGSRGKAGAAVLA
                     GHAALRAGAGLATVAVARSLLASVSARRPELMTEPLPETRAGAPARAAARRVLTLLAG
                     CDALAIGPGLGTSAETAAAVMAILRGRMVPAVADADALNVIAAARRGRSTAAISARRP
                     LVLTPHPGEASRLLGRSTETVQADRLDAARTLAGISRAVVVLKGHRSLIATPDGRVAV
                     NASGNPGMATAGTGDVLTGVLGAFLARGMSAWDAARLAVFVHGAAGDRAAHERGEDGM
                     IAGDLLDRLPDAMLSLATVGTRDDG"
BASE COUNT          354 a          843 c          823 g          291 t
ORIGIN      
        1 agaagcagat caagtacccg ttggcctgca aggacgactt cggccgcctg cgcgtcggcg
       61 ccgcgatcgg catcgcgcgc gacacgcagg accgggccga tgcgctgatc gacgccaacg
      121 tcgacgtgct ggtcgtcgac accgcgcacg gccacagcga gggcgtgctc accaccgtcg
      181 cgcgactgcg cgagcgtcac ccggagatcc agctggtcgg cggcaacgtc gcaacggcgg
      241 acggcgcgaa ggcgttgatc gagcgcggcg tcgacgcggt caagatcggg gtcggccccg
      301 gcagcatctg cacgacgcgg atcgtcaccg gcgccggcgt accccagctg tcggcgatcc
      361 tcgccgcgcg cgacacctgc cgcgaggccg acgtcccgtt ggtcgccgac ggcggcatca
      421 agtactcggg cgacatcacc aaggcgatcg cagccggcgc aagcgcggtg atgatcggca
      481 gcctgttcgc cggggccgag gaatcgccgg gcgagacggt gttgttccag ggccggacct
      541 tcaaggaata ccgcgggatg ggctcgatcg gcgcgatgaa gaaggggtcg cgcgatcgct
      601 acttccagga agacgagttc gacgagcgaa agctcgtgcc ggaggggatc gaaggccggg
      661 tcccgtacaa gggaccgctg tcggcgctgg tctcccagct cgtcggcggc ctgaaggccg
      721 ggatgggcta cctcggctgc ccggacatcg aagcgctgca gcggaacgcg cggttcattc
      781 gcctgacccc ggccggtctg cgcgaatcgc acgcccacga cgtgatcatc accaaggaag
      841 caccgaacta ccgcctcgat tgaccaggct gctaggcgtc gccgcgccgc tcggtcggac
      901 ggtcccgctc gaggcccgag atctcgatgc ggcgcgaatc ctcgccgaca tcgtgcaggc
      961 gcacgacgat cgcgtcgcgc cgatagcgcg gcgggatctt ctcgccccac tcgaccagca
     1021 cgacggctcg gtcggtctgg atctcctcga gacccagagt cgcgatctcc tcgtccgagg
     1081 cgagacgata cagatcgacg tggaacatcg tcagccggcc accgcggtat tcctggatca
     1141 aggtgaacga cggcgagctg acgtcctcgg gtgcgagccc gaggccgatc gcgacgcccc
     1201 gagcgaagac ggtcttgccc agaccgagat cgccctcgag caggatcaac tcgccgccct
     1261 tgagcttgcg tgcgaccgtg cggccgagct cgagcgtctc gtcctcgctg agcgagaaga
     1321 cggtgcgcga cgaatcgcct ccgtcggttt cccgcgagcg ggagtgcggt gtgatctccg
     1381 aaggcatgtc gaccatctcg agcctacccg tcgtctcgcg tcccgaccgt tgccagggac
     1441 aacattgcgt ccggaagccg gtccagcaag tcgccggcga tcatgccgtc ctcgccgcgc
     1501 tcgtgggccg cgcgatcccc cgccgccccg tggacgaaga cggcgagccg agcggcgtcc
     1561 catgccgaca tcccccgtgc gaggaacgcg ccgagcaccc cggtgaggac gtcgccggtc
     1621 cccgccgtcg ccatccccgg attgcccgag gcattgacgg cgacgcggcc gtcgggtgtc
     1681 gcgatcaacg agcgatgccc cttcagcacg acgacggcgc ggctgatgcc ggccagcgtg
     1741 cgggcggcgt cgagccggtc ggcctggacg gtctccgtcg accggccgag caggcgggac
     1801 gcttcgcccg ggtgcggggt caacacgagc ggccgccgcg cggagatcgc cgcggtcgac
     1861 cggccacgac gggcggcggc gatcacgttc agcgcgtcgg cgtcggcgac ggccgggacc
     1921 atgcggccgc ggaggatcgc catcaccgcc gccgccgtct cggccgaggt gccgaggccc
     1981 gggccgatcg ccagcgcgtc gcaaccggcg agcagcgtca gcacgcgtcg ggcggccgcg
     2041 cgcgccggcg caccggcgcg cgtctccggc agcggctcgg tcatcagctc cggacgccgc
     2101 gccgacacgc tggccaagag cgagcgggcg accgccaccg tcgccagccc cgccccggcg
     2161 cggagcgcgg cgtgcccggc gagcaccgcc gcgcccgcct tgccccgcga tccggcgacc
     2221 accagcagat gcccgtaggt tcccttgtgc gagtcggccg cgcgccggcc gagcaggggg
     2281 gcggcggccg ccgcgtccca ccactcgagc c
//
LOCUS       DASPGN010000085        12172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1069271, whole genome shotgun sequence.
ACCESSION   DASPGN010000085 DASPGN010000000
VERSION     DASPGN010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12172
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069271"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VD788_02370"
     CDS             complement(<1..1043)
                     /locus_tag="VD788_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXV75135.1"
                     /translation="MLAARSVSIAVVLAALVAPACGPPASRSDEWEALDSSASAVVVM
                     RRAADAAELLGEIRRRTETATVTLDPFADDGELGRMNRVAPDRFYEIEQVDLFACLRL
                     ALDYAEESDGAYDPTIGALDSLYASRLETAEPPRPAEIDVVLTQVGWEKVTVEPEVRA
                     VRYRAPRLVVDLGPVAVGCAVDWAARAFARPGSIGGLIRVGGAHRAWGRPPDRPSWAV
                     GLDDPRSPGRTLVELDVTNRGVAVCGQPATSESSGQARLREIDVLDPRTGRPAATDLL
                     AVVTTADSAADAAALCHVIHVAGSLAGTDIFREMRRAETVMLVRADDGPPYLVASASL
                     RGRLRLSEALSAEV"
     gene            1102..4692
                     /locus_tag="VD788_02375"
     CDS             1102..4692
                     /locus_tag="VD788_02375"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HXV75136.1"
                     /translation="MSRFGHNRGYIDELYRSFLDDPTSVSEAWREFFRDYRADGETTR
                     RHDRPATETASPPATDAEPPPTRLVGISARIAENMNTSLAMPTATSARSIPVKMLEEN
                     RRVINAHQTTVSGPRISFTHLIAWGIVRALERHPAMNVAYLERDGQPHVVRRERVQLG
                     LAIDVERDGERILLVPNIKDAASLSFPALVAAYDDLVARARQNKLTVEDFERTTITLT
                     NPGMLGTALSVPRLMKGQGVIIGTGTIGYPAEYSGMAREMTSALGISKTMTVTSTYDH
                     RVIQGAESGGFLFTLERLLRGEQGFYERLFADLGVPQEPFEYAGDTNPIAFGGAGSDE
                     AIAKQAGVLQLIRAYRVRGHLWADLDPLHYRPEPTPELEMSTYGLTVWDLDREFIAGG
                     LGGNHGKLPLREILETLQRTYCWHVGVEYMHIQDTDNRQWLQERMESQERSEPLDRET
                     QIRILERLNVAEAFETFLHTNYVGQKRFSLEGCETLIPVLDALLDDAAASGIDEVVMG
                     MAHRGRLNVLAHVVGKPYGEIFREFEGIDPLSTHGSGDVKYHLGARGVYTTSDGQRIP
                     IELASNPSHLEAVDPVVQGMARARQDKLADREHGRVLPVLIHGDASFAGQGVVAETLN
                     ASQLSGYRTGGTVHVIVNNQIGFTTGPADARSSTYPTDVAKMIRAPIFHVNGDYPEDA
                     VRVIRLALAFRQRFKRDVVVDLFCYRRWGHNEADDPSYTHPILYSRIKDHRSVRKLFT
                     ERLIRRGDLDADQAERALGHFRSQLQQVHDDVRAAERGSENAAAEHPPAPEPVAEPGD
                     TSVERTTLERVLDGMLATPPGFHLHPKLERQLAKRRQRFGQDRIDWALAESLAFGSLV
                     LGGVPVRLSGEDSIRGTFSQRHTALFDFETGDRWVPLAHLGAGQAAFAAHDSQLSEFA
                     VMGFEYGYSVNYPEALVLWEAQFGDFVNGAQVIIDQFLVSAESKWNQRSALVLLLPHG
                     YEGQGPEHSSARLERFLQLAAEDNIRVAYPTTPAQYFHLLRRQALDARRKPLVVMTPK
                     SLLRLPECVSPVERLTSGRFDSVLEDPRCASPEAIDRLIVCSGKIYYDLDAFRREHDS
                     PGTALLRIERLYPFPEQRLRQLIGGHAALREVVWAQEEPRNMGLWEFVRTRLEPLLPS
                     AVGLSYVGRPAGASPATGSSRRHAAQQQRLVREAIGASADRRSAAETGKV"
     gene            complement(4703..6088)
                     /locus_tag="VD788_02380"
     CDS             complement(4703..6088)
                     /locus_tag="VD788_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HXV75137.1"
                     /translation="MIENQRESELRTAPSDAPPSPPQPTAADDTPEERPGGTNGSSAE
                     ATAPSVDPPPVREPAAEKTPPATRPQVASLYRALRSARPVRGTITKVIKGGYEVYLGK
                     TRGFCPHSQIDLQRVDRPEQHVGQTYSFRITQIRRGGQDVVVSRRALLEDRRRDEASA
                     VRATLVEGAVTRGHVAAVADFGAFVDLGAGVRGLVHVSELSHSRVTRVKDVVKVGDEV
                     QARILKLDAATGKISLSIRQAIDDPWADVEQRFAPGRVYRGVVRRMTAFGAFVELAPG
                     VEALAPASEFPPSERPWREQLAVGDQRDWLVLSVQGATRRISVTPPVEGVDLSTLAEI
                     GSGTKLKGKVQRVEDYGVFVWLGPGRVGLMPNALSGVPRGTEMARRFPVGSEIEVEVR
                     ELDPDGRRIRLASPRSEPPAVPRPPREPRAKHAKETLPLTPAPATGVSTFGTSLADKL
                     RAALAESDRER"
     gene            6249..7223
                     /locus_tag="VD788_02385"
     CDS             6249..7223
                     /locus_tag="VD788_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HXV75138.1"
                     /translation="MRAIVVTRPGAPGVLALREVSDPVAGPREVVVRVVAAGVNRADL
                     LQRLGLYPAPAAAPGPGLEFAGYVAETGAGVRLLAPGDRVMGIVSGGAYAERVAVDER
                     LCMIVPASVDWVTAAAVPETFLTAYDALFRRVSLTVGETLLLQAAGSGVGTAALQLGL
                     IAQARIVALSRSAAKREALSKIGAQHVLDPSAPSAVDEVLAATAGCGVDVVLDLVGAS
                     AFEFHLRVLRERGRWVLVGLLGGSRREVDLFQLMRKRLTLIGTVLRSRTLEQKIALVE
                     EFSARILPLIADGRVSPVVHGVLDLDRAAEAHALMERGENLGKIVLRV"
     gene            7226..8140
                     /gene="murB"
                     /locus_tag="VD788_02390"
     CDS             7226..8140
                     /gene="murB"
                     /locus_tag="VD788_02390"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HXV75139.1"
                     /translation="MDRTVDELRRELTARGFRGELVAESPLAPHTTWKIGGPAELMAT
                     PADVDDVVLAVERAAAAGVAWRVLGNGSNLLVADRGVRGLVIRVRRVLDAVRVEGHTL
                     DVGAGAMLPLVAHRAAAAGLSGLEFGAGIPGTIGGAVIMNAGWHEFEIGNLVRWVDWI
                     GPSGQRLRQERDDCGFRYRGSRFRDERGIVVAANLALEPGDAREIRARLDRFAASRKQ
                     NQPTELPSCGSVFLQPPGDFAGRLIEQAGLKGSTNGAAQVSPKHANFIVNLGGATSRQ
                     VLELVERVERAVEDRFGVRLVREFEYWR"
     gene            complement(8142..10724)
                     /locus_tag="VD788_02395"
     CDS             complement(8142..10724)
                     /locus_tag="VD788_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXV75140.1"
                     /translation="MARSATKLRPRRPAGLPAHPLLLATDLRTWAGDESAASGRREAL
                     LGRVSFLRLAPSGRLIEAKVRGNRTAPYHVQVWVEDERIHTRCTCAGGARSGCKHAVA
                     ALETMRFPLSAAAPASVARRSRVTARKHDRGFDAGAAQSGYLLFGGAERSLTREERIE
                     AARQLELTLRRGKARKEREPVVALAPDGGPPRFEVGARDKHRVTLRGDNLALAACTCD
                     DYAHGELHSCAHIERVRMWYLRKKKQIPTRLLSVWWRPRVWTDRQPETLREIRADAAD
                     QPLPDELAPFFDRSGWLAPAPPDRDGAAWLRDAVEASCEAARGRGWVWDLDAGVASWI
                     DQQDRERHHESLLDTISRATPAWHAIVARLGFRLHPYQETGALFLARRGRALLADDMG
                     LGKTVQAIVAALLLRMVAGARRALVVCPASLKHQWRREIEKAWGERAIVVESGRAARL
                     ATYRAWTDGFLILNYELVLRDLDDVRAVRPDLVILDEAQRIKNWDTKTAIAIKRLDSR
                     HAFILTGTPLENRLIELHSLAEHLHPRALGPRWRLLPYHAVTDLQGRAIAYEGLEVLR
                     KRMSGFFLRRERRKVLDQLPERTDNTFWTGMTPSQQRPYRRHAATVASLVARHHNLRP
                     GDIRLLLQSLTSMRILCNAYAQYDFALFEQRLRDPAPPTRHELRALGSPKLEEFVRVL
                     EDLIDDAGGKIVVFSQWERMLRLAHYVVREPLAQRELRADVFHGGLNARARDEMLEAF
                     RVDPEFHVLFSTDAGGLGLNLQESASIVINLEVPWNPAVLEQRIGRVHRLGQLRSVQV
                     LYLVTRGAVEERVRQVVEGKRALFDGLLTEQADRVVFDEPLRASLVRRVRDLIESGEG
                     AGAI"
     gene            11314..>12172
                     /locus_tag="VD788_02400"
     CDS             11314..>12172
                     /locus_tag="VD788_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXV75141.1"
                     /translation="MDRLDRAELPAAIDEERGDVARRIRRALNDRLPADTPETTPRVG
                     AAERLQEAIERLVQDVDGFLAREAIAASLTADERRELLRGMMLTRATDNQLKAFFLGG
                     DVRYGGVSFQGKGFRSLGQEAIYAAPIRLRRGREYRDARGAWHGDMVAPLIRDAGAAL
                     AMDPSAETVRMILNAQMGKAGPPMNGKDLHIGDLSLGIVPAAAPLSISSLTVAGIALG
                     FARRGEDRVALAFIGEGGSSLGEWHEAINLCAARRLPAVFCVQNNQTALSTPVAEQSA
                     VRVFADKADG"
BASE COUNT         1854 a         4183 c         4272 g         1863 t
ORIGIN      
        1 acctcggccg aaagcgcctc ggacaagcgc agccggccgc gaagcgacgc cgaggcgacg
       61 aggtacggcg gcccgtcgtc ggcgcggacc agcatcaccg tctcggcgcg acgcatctcg
      121 cggaagatgt ccgtcccggc cagcgagccg gcgacgtgga tcacgtggca cagcgcggcg
      181 gcgtcggccg cgctgtccgc ggtcgtgacg accgccagca ggtcggtggc cgcgggccgt
      241 ccggtccgcg gatcgagcac gtcgatctcc cgcagccgcg cctggccgct actctcgctc
      301 gtcgcgggct gcccgcagac cgcgaccccg cgattcgtca cgtcgagctc gaccaacgtt
      361 cggccgggcg aacgcgggtc gtcgaggccc acggcccagc tcggtcggtc gggcggtctg
      421 ccccacgcgc gatgagcgcc gccgacgcga atcagcccgc cgatgctccc cgggcgcgcg
      481 aacgcgcgcg ccgcccaatc gaccgcgcag ccgacggcga ccggtcccag gtcgacgacc
      541 aaccgcggcg cacgataccg gacggcccgc acctccggtt cgacggtgac cttctcccag
      601 ccgacctgag tcaataccac gtcgatctcg gccgggcgcg gcggctcggc ggtctcgagg
      661 cgagaggcgt acaggctgtc gagggcaccg atcgtcggat cgtacgcgcc gtcgctctcc
      721 tcggcgtagt cgagcgccag gcgcaggcag gcgaacaggt cgacctgctc gatctcgtag
      781 aaccgatccg gcgcgacgcg gttcatccgg ccgagctcgc cgtcgtcggc gaacgggtcg
      841 agggtgacgg tcgccgtctc cgtacggcgg cggatctcgc cgagcagctc cgccgcgtcc
      901 gcggcgcgac gcatgacgac gacggcggac gccgagctgt ccagcgcctc ccactcgtcc
      961 gaccgcgagg cgggcggccc gcaggccggt gcgacgagcg ccgccaacac gaccgcgatc
     1021 gacacgctgc gagccgcgag catcggcgcc tccttccggg gtcattgccg ccggcgccca
     1081 acacctttac gatacccgat catgagccgc ttcgggcaca atcgcgggta catcgacgag
     1141 ctgtaccgga gctttctcga cgatccgacg tcggtcagcg aggcgtggcg cgagttcttt
     1201 cgcgactatc gcgccgacgg cgaaacgact cgccgtcacg atcggccggc gacggagacc
     1261 gcgtcgccgc cggcgaccga cgccgagccc ccgccgacgc ggctcgtcgg catctcggcg
     1321 cgcatcgccg agaacatgaa caccagcctg gcgatgccga ccgcgacctc cgcgcggtcg
     1381 atcccggtca agatgctcga agagaatcgg cgcgtgatca acgcgcacca gacgaccgtg
     1441 tccggcccgc gcatctcgtt cacgcacctg atcgcgtggg gtatcgtccg ggcgctcgaa
     1501 cggcacccgg cgatgaacgt ggcctatctc gagcgcgacg ggcaaccgca cgtggtgcga
     1561 cgcgagcgcg tccagctcgg tctcgcgatc gacgtcgagc gagacggcga gcggatcttg
     1621 ctcgtgccca acatcaagga tgccgcgagc ctgagcttcc cggcgttggt ggcggcctac
     1681 gacgacctcg tcgctcgggc caggcagaac aagctgacgg tcgaagactt cgagcggacg
     1741 acgatcacgt tgaccaaccc cggaatgctg ggtacggcgc tgtccgttcc gcggctgatg
     1801 aaaggccagg gcgtgataat cggcaccggc acgatcggct atccggcgga gtactccggg
     1861 atggcgcgcg agatgacgtc ggcgctcggc atctcgaaga cgatgaccgt caccagcacc
     1921 tacgaccacc gggtgattca gggtgcggag tccggcggtt tcctgttcac gctcgagcgc
     1981 ctgctgcgcg gcgagcaggg gttctacgaa cggttgttcg cggatctcgg cgtcccgcag
     2041 gagccgttcg agtacgcggg cgacaccaat ccgatcgcgt tcgggggcgc gggcagtgac
     2101 gaggcgatcg ccaaacaggc gggagtgctg cagttgatcc gtgcctaccg cgtgcgcgga
     2161 cacctgtggg ccgacctcga tccgttgcac taccggcccg agccgacgcc ggaactcgag
     2221 atgtcgacct acggcctgac cgtctgggat ctcgatcgcg agttcatcgc cggcgggctg
     2281 ggcggcaacc acggcaagct gccgctgcgc gagatcctcg agaccctgca acgcacctac
     2341 tgctggcacg tcggcgtgga gtacatgcat atccaggaca ccgacaaccg acagtggctc
     2401 caggaacgga tggagtcgca ggagcggtcc gagccgctgg atcgcgagac ccagatccgc
     2461 atcctcgaac ggctcaacgt cgccgaagcg ttcgagacct tcctccacac gaactacgtc
     2521 ggccagaagc gcttctcgct cgagggctgc gagacgctga tacccgtgct cgacgcgttg
     2581 ctcgacgacg ccgcggcgag cgggatcgac gaggtcgtga tggggatggc ccatcgcggg
     2641 cggctgaacg tgctggccca tgtcgtcggc aagccctacg gtgagatctt ccgcgagttc
     2701 gaggggatcg atccgctgtc gacccacggg tcgggcgatg tcaagtatca cctcggagcg
     2761 cgcggcgtct acacgacgag cgacggccag cggatcccga tcgagctggc gtcgaacccg
     2821 agccacctcg aagcggtcga cccggtcgtc caggggatgg cgcgggcgcg gcaggacaag
     2881 ttggccgacc gcgagcacgg ccgggtgctg ccggtgctga tccacggcga cgcgtctttc
     2941 gccggtcagg gcgtggtcgc cgagaccctc aacgcatctc agctgagcgg ctatcgcacg
     3001 ggtgggacgg tgcacgtgat cgtcaacaac cagatcggct tcaccaccgg accggcagac
     3061 gcgcgatcgt cgacctaccc gaccgacgtg gcgaagatga tccgtgcacc gatcttccac
     3121 gtcaatggcg actatcccga ggacgcggtg cgcgtgatcc gtctggcgct ggcgttccgc
     3181 caacgattca agcgcgacgt ggtggtcgac ctgttctgtt accgccgttg gggtcacaac
     3241 gaggccgacg acccgtccta cacgcacccg atcctctatt cgcggatcaa ggaccaccgc
     3301 tcggtccgca agctgttcac ggaaaggctg atccggcgcg gcgacctcga cgccgaccaa
     3361 gccgagcggg cgctcgggca ttttcgcagc cagctgcagc aggttcacga cgacgtccgc
     3421 gcggccgagc gcgggtcgga gaacgcggcg gccgagcacc cgccggcgcc ggagcccgtc
     3481 gccgagcccg gcgacacttc cgtcgagcgg acgacgctcg agcgcgtgct ggacggcatg
     3541 ctcgccacac cgcccggctt tcatctgcac cccaagctcg aacgccagtt ggcgaagcgt
     3601 cgtcagcggt tcgggcagga tcggatcgat tgggcgctcg cggagtcgtt ggccttcggc
     3661 tcgctcgtgc tcggcggcgt tcccgtgcgg ctgagcggcg aagacagcat ccgcggaacg
     3721 ttcagccagc gtcacaccgc gctgttcgac ttcgagaccg gcgatcgctg ggtgccgctc
     3781 gcgcatctcg gcgcgggtca ggcggcgttc gcggcgcatg acagccaact gtccgagttc
     3841 gcggtgatgg ggttcgagta cggctacagc gtgaactacc cggaagcgct ggtgctatgg
     3901 gaggcccagt tcggcgattt cgtcaacggc gcgcaggtga tcatcgatca gttcctggta
     3961 tcggcggaat cgaagtggaa ccagcgaagc gcgctcgtgc tgttgctgcc ccacggttac
     4021 gaaggccagg ggcccgaaca ctccagcgcg cggctcgagc gctttctcca actcgcggcc
     4081 gaggacaaca tccgtgtggc ctatccgacg acgccggcgc agtacttcca cctgctgcgg
     4141 cgacaagcgc tcgatgcccg gcgcaagccg ctggtcgtga tgacaccgaa gagcctgctc
     4201 cgtctgcccg agtgcgtctc gccggtcgag cggttgacct ccggtcggtt cgacagcgtg
     4261 ctggaagatc cgcgctgcgc ctcgcccgag gcgatcgatc ggttgatcgt ctgctcggga
     4321 aagatctact acgacctcga cgcgttccgc cgggagcacg actcgccggg gacggcgctt
     4381 ctgcggatcg agcggctcta cccgttcccg gagcaacggc tgcgccagtt gatcggcggc
     4441 catgccgcgt tgcgcgaggt cgtatgggcc caggaggagc cgcgcaacat gggcctctgg
     4501 gagttcgtcc ggacacggct cgagccgcta ctgccgagtg cggtcggctt gagctacgtc
     4561 ggccgccccg ccggcgccag cccggccacg ggctcgtctc gccggcacgc cgcccagcag
     4621 caacgcctcg tccgtgaggc gatcggggcg agcgccgacc gacgcagcgc ggcggagacg
     4681 ggcaaggtct gagtcgtcgg gttcagcgct cgcggtccga ctcggccagt gccgcacgga
     4741 gcttgtcggc gaggctggtg ccgaaggtcg aaacgccggt ggccggcgcc ggggtcagcg
     4801 gcaacgtctc cttcgcgtgc ttggcccggg gctcgcgcgg cggacgcgga acggccggag
     4861 gctccgaccg tggactggcc agccggatgc ggcgaccgtc tgggtccaac tcgcgcacct
     4921 cgacctcgat ctcggagccg accgggaagc gccgagccat ttcggtgccg cgcggcacgc
     4981 cggacaacgc gttgggcatc agcccgaccc gtccggggcc gagccagacg aagacgccgt
     5041 agtcctcgac ccgttgcacc ttgcccttca gcttggtgcc gctaccgatc tcggccagcg
     5101 tcgacagatc gaccccttcg accggcggcg tgaccgagat ccggcgggtc gctccctgga
     5161 ccgacagcac gagccaatcg cgttgatccc cgaccgcgag ttgctcgcgc cacgggcgct
     5221 cggaaggtgg aaactcgctc gccggagcca acgcttcgac acccggcgcc agctcgacga
     5281 acgcgccgaa cgcggtcatc cgccgcacga ccccccggta gactcgaccc ggagcgaacc
     5341 gctgttcgac gtcggcccac ggatcgtcga tcgcctggcg gatgctgagc gagatcttgc
     5401 cggtcgcggc gtcgagcttc aggatgcgcg cctgaacctc gtcgccgacc ttgacgacgt
     5461 ccttcacgcg ggtcacgcgg gagtgggaca gctcggagac gtgtaccagt cctctcaccc
     5521 cggcaccgag atcgacgaac gcgccgaagt cggcgaccgc cgcgacgtga ccgcgcgtga
     5581 ccgcgccctc gacgagcgtc gcgcggacgg ccgacgcctc gtcgcgacgc cggtcctcga
     5641 gcaacgcgcg ccgcgacacg acgacgtcct gtccgccgcg gcgaatctgc gtaatgcgaa
     5701 agctgtaggt ctggcccacg tgttgctcgg gtcgatcgac ccgctggagg tcgatctgcg
     5761 aatggggaca gaacccgcgc gtcttgccga gatagacctc gtagccgccc ttgatgacct
     5821 tggtgatcgt tccccggacg ggacgtgcgg agcgcagcgc gcggtacagc gaggcgacct
     5881 gcgggcgagt tgccggtggg gttttctccg ccgccggctc gcgcactggc ggggggtcga
     5941 cggacggcgc ggtcgcctcg gccgagctgc cgttggtgcc accggggcgt tcctcgggcg
     6001 tgtcgtcggc tgcggtgggc tgcggtgggg acggcggggc gtcgcttggg gccgtgcgta
     6061 gctcggactc gcgctggttc tcgatcactg ttccgcctcg tcgtcgatcc ggccacgacc
     6121 gtctaccgcc ggcgcatccg acccgacggg actcatccga ctctgtttat atgccgtgcg
     6181 tcggatgatt ctgccacaaa aggatgcatg cggaacaaaa caaccgacgc tgtggcatcc
     6241 tgaggccgat gcgagcgatc gtcgtcacgc gaccgggtgc gccgggggtg ctggcgctcc
     6301 gggaggtgtc ggatcccgtc gccggcccgc gcgaggttgt ggtgcgcgtc gtagcggcgg
     6361 gcgtgaatcg cgccgacctg ctgcagcgtc tcgggctcta tccggcaccg gccgcagcgc
     6421 cgggccccgg tctcgagttc gccggatatg tagcggagac gggcgcgggc gtccggctgc
     6481 tcgcgcccgg cgaccgcgtg atgggaatcg tctccggcgg tgcctacgcc gagcgggtgg
     6541 ccgtcgacga gcggctgtgc atgatcgtgc cggcgtcggt cgactgggtc accgcggccg
     6601 ccgtgcccga gaccttcctt accgcctacg atgccctgtt ccgccgcgtg tcgctgacgg
     6661 tcggtgagac gttgctactg caggcagccg gctccggggt cggcacggcg gcgctgcagc
     6721 tcggcttgat cgcgcaggcg cggatcgtcg ctctgtcgcg gagcgccgcc aagcgcgagg
     6781 cgttgagcaa gatcggcgcc cagcacgtgc ttgacccgtc ggcgccgagc gcggtcgacg
     6841 aagtgctggc cgcgaccgcc gggtgcgggg tcgatgtcgt gctcgatctc gtcggcgcat
     6901 ctgcgttcga attccatctg cgggtgctgc gtgagcgcgg gcgttgggtg ctggtcggtc
     6961 tgctcggcgg atcgagacgg gaagtcgatc tgtttcagct gatgcgcaag cgtctgaccc
     7021 tgatcggtac cgtgctgcga tcgcgcacgc tcgagcagaa gatcgcgctc gtcgaggagt
     7081 tttcggcgcg gatcctgccg ttgatcgccg acggacgcgt ctcgccggtg gtgcacggcg
     7141 tgctcgatct cgatcgcgcg gccgaagccc atgcgctgat ggagcgcggc gagaatctgg
     7201 gcaagatcgt gctgcgggtc tgaggatgga ccgcacggtc gacgagctgc ggcgcgagct
     7261 gaccgcccgc ggctttcgcg gcgagctggt cgcagaatcc ccgctcgccc cgcatacgac
     7321 gtggaagatc ggcggcccgg ccgaactgat ggccaccccg gccgacgtcg acgacgtggt
     7381 gctggcggtc gagcgcgccg ccgctgccgg cgtggcgtgg cgcgtgctgg ggaacggctc
     7441 gaacctgctg gtcgccgacc gtggtgtgcg cggtctggtc atccgcgttc gccgggtgtt
     7501 ggacgcggtg cgggtcgaag gtcacacgct cgacgtcggg gccggagcga tgctgccgct
     7561 ggtcgcgcat cgcgcggcgg cggcggggct ctccggactc gagttcggcg ccgggattcc
     7621 cgggacgatc ggcggtgcgg tgatcatgaa cgccggctgg cacgagttcg agatcggcaa
     7681 cctcgtccgc tgggtggatt ggatcggacc gtccgggcaa cggctccggc aagaacgaga
     7741 cgactgtggc tttcgctacc gcggcagccg ctttcgcgac gaacgcggga tcgtggtggc
     7801 cgcgaacctg gcgctcgagc cgggggatgc gcgggagatc cgcgcgcggc tggatcgatt
     7861 cgcggcctcg cgcaagcaga accagccgac cgagctcccg tcgtgcggat cggtctttct
     7921 gcagccgccg ggcgatttcg ccggccggtt gatcgagcaa gccgggctga agggatcgac
     7981 gaacggcgcg gcacaggtga gccccaagca cgcgaacttc atcgtcaatc tgggcggtgc
     8041 gaccagccgg caggtgctgg aactggtcga gcgcgtcgaa cgcgcggtcg aggatcgctt
     8101 cggcgtccgt ctcgtgcgcg agttcgagta ctggcgctag tctagatcgc gcctgcaccc
     8161 tctccgctct cgatcaggtc ccgcacgcgt cgcaccagcg acgcgcgcaa cggttcgtcg
     8221 aacacgacgc gatcggcctg ttcggtcagc agcccgtcga acaacgcccg tttgccctcg
     8281 acgacttggc gcacgcgctc ctcgaccgcg ccgcgggtga cgaggtacag cacctggaca
     8341 ctgcgcagct gaccgaggcg atgcacccga ccgatccgtt gctcgagcac agccggattc
     8401 cacggcactt cgaggttgat gacgatcgat gcggattcct gcaagttcaa gccgagcccg
     8461 cccgcgtcgg tcgaaaacag gacgtggaac tccgggtcga cccggaatgc ttcgagcatc
     8521 tcgtcccggg cgcgggcgtt caatccgccg tggaacacgt cggcgcgcag ctcgcgttgg
     8581 gcgagcggct cgcgaacgac gtagtgcgcc aggcgcagca tccgttccca ctggctgaag
     8641 acgacgatct tgccgcccgc gtcgtcgatc aggtcttcga gaacccggac gaactcctcg
     8701 agcttgggcg agccgagtgc gcgcagctcg tggcgcgtgg gcggagccgg gtcgcgcaat
     8761 cgttgctcga acagcgcgaa gtcgtactgg gcgtaggcgt tgcacagaat gcgcatgctg
     8821 gtcagcgatt ggagcagcaa ccgaatgtcc ccgggtcgca agttgtggtg tcgcgcgacc
     8881 agcgacgcga ccgtcgcggc gtgtcggcgg tacggccgct gttgcgacgg ggtcatcccg
     8941 gtccagaacg tgttgtccgt ccgctccgga agctggtcga gcaccttgcg acgctcgcgt
     9001 cggaggaaga accccgacat gcgcttgcgc agcacctcga gaccctcgta ggcgatcgcg
     9061 cgcccctgga gatcggtgac ggcgtgatac ggcagcagcc gccagcgcgg tcccagcgcg
     9121 cgcgggtgga gatgctcggc cagcgaatgc agttcgatca ggcgattctc gagtggcgtt
     9181 ccggtgagga tgaatgcgtg gcggctgtcg agtcgcttga tcgcgatcgc ggtcttggtg
     9241 tcccagttct tgatgcgctg cgcctcgtcc aggatgacga gatcgggccg gaccgcacgc
     9301 acgtcgtcga gatcccgcaa gaccagttcg tagttcaaga tcagaaagcc gtcggtccac
     9361 gcgcgatagg tcgccaggcg ggcggcccga ccactctcga cgacgatcgc ccgttcgccc
     9421 caagcttttt cgatttcgcg tcgccactgg tgtttgagag acgccggaca gacgacgagc
     9481 gcgcgccggg cgcccgcgac catccgcagc agcaacgcgg cgacgatcgc ctggaccgtc
     9541 ttgcccagcc ccatgtcgtc ggcgagcagc gcccgaccgc ggcgcgcgag gaacagcgcg
     9601 ccggtttcct gatagggatg caggcggaag cccagccggg cgacgatcgc gtgccacgcc
     9661 ggcgtcgcgc ggctgatcgt gtcgagcagg gactcgtggt gccgttcgcg atcctgctgg
     9721 tcgatccacg aagccacgcc ggcgtcgagg tcccagaccc agcccctgcc gcgcgccgcc
     9781 tcgcacgatg cctcgaccgc gtcgcgcaac cacgccgcgc cgtcgcgatc gggcggggca
     9841 ggcgccagcc agccggagcg gtcgaagaac ggcgccagct cgtcgggcaa cggctgatcg
     9901 gccgcatcgg cccgaatctc gcgcagtgtt tccggctggc ggtcggtcca gacccgcggc
     9961 cgccaccaga cggagagcag gcgggtcgga atctgcttct tcttgcgcaa gtaccacatt
    10021 cgcactcgct cgatgtgtgc gcaggagtgc agctcgccgt gcgcgtagtc gtcgcacgtg
    10081 caggcggcga gcgcgagatt gtcgccgcgc agtgtcacgc ggtgcttgtc acgcgcgccc
    10141 acttcgaacc gcggcgggcc gccgtcgggc gcgagcgcga ccaccggctc ccgctccttg
    10201 cgcgccttgc cgcggcgcag cgtcagttcg agctgccgcg ccgcctcgat gcgctcctcg
    10261 cgggtgagcg agcgctccgc gccgccgaac agcagataac cggattgcgc ggccccggca
    10321 tcgaacccgc gatcgtgctt cctcgcggtc acacggctgc gtcgggcgac gctcgccggc
    10381 gcggccgccg acagcggaaa ccgcatcgtc tcgagcgcgg cgaccgcgtg cttgcaccct
    10441 gaacgcgcgc ccccggcgca cgtacaacgg gtgtgaatcc gctcgtcctc gacccagacc
    10501 tgaacgtgat agggcgcggt gcggttgcct cgcaccttgg cttcgatcag gcgtccgctc
    10561 ggcgcgagtc gcaggaatga cactcgtccg agcaacgctt cgcgccggcc gctcgccgcc
    10621 gattcgtcgc cggcccaggt tctgaggtcg gtggcgagca gcagcggatg ggccggaaga
    10681 cccgctgggc gtcgcggtcg gagcttcgtg gcggatcggg ccaagtctcg ggctctcccg
    10741 gaatgggacg gtcgaaacaa acagtctgcc gcacggcggg cggtccatca acacaaggtg
    10801 gtgcgcagct tggtggtcgg gattgtccgg tgtacgtccg catgcggaca gcgggcggac
    10861 gatcgtgacg gaatgccggc cgaatcgggt cggcatcgtc tttgccagcc ggcgagggat
    10921 catgctccga cctcgtccgc cgatcgagac cctccgtccg ggtcgcctca acccccccgg
    10981 ctgaagcgat ttgccaccgc ctctggatca cacccgccgt cggacccaac acccgtcacc
    11041 agctcgtcca cgccttctga tcaccacacc accccgagat ccgccccaat ccccctccca
    11101 ccgctcccaa aacccgcacc gaaccgacac acacaccaac aagctccgca ccacgttgtg
    11161 tagactacgt ggatgggtag atcggtgact cgccgaagaa aaaccgcccc cagccgtccc
    11221 ccacgactcc cagcacggct cgccgcgttc gtcaccgaac ggcatccgtt cgcggccggt
    11281 cgggtcgcgc aggcaacggt ggggctcggc cggttggacc gattggaccg cgccgagctt
    11341 cccgcggcga tcgacgagga acggggcgac gttgcccgac gcatccgccg cgcgttgaat
    11401 gaccggctgc ccgcggacac gccggaaacg acgccgcgcg tgggtgccgc cgagcgcttg
    11461 caagaggcga tcgagcgcct cgtgcaggac gtcgacgggt ttctcgcgcg cgaggcgatc
    11521 gcggcctcgc tgacggccga tgagcgtcga gagttgctgc ggggcatgat gttgacgcgt
    11581 gcgaccgata accagctgaa ggcgttcttt ctcggcggcg acgtccgcta cggcggggtt
    11641 tcgttccaag ggaagggctt tcggtcgctc ggccaggagg cgatctacgc cgccccgatc
    11701 cgcctgagac gcggtcgcga gtaccgcgac gcccgcgggg cgtggcacgg tgacatggtg
    11761 gcgccgctga tccgcgatgc cggggcggcg ctcgcgatgg acccctcggc cgagacggtc
    11821 cgcatgatcc tcaatgcgca gatggggaag gccggtccgc cgatgaacgg caaggacttg
    11881 cacatcggcg acctgtcgct ggggatcgtg ccggccgcag ccccactctc gatctcgtcg
    11941 ttgaccgtcg ccgggattgc cctggggttt gcccggcggg gcgaggaccg cgtggcgctg
    12001 gcgttcatcg gcgagggtgg ctcgtcgctc ggtgaatggc acgaggcgat caacctgtgc
    12061 gcggcgcgcc ggctaccggc cgttttctgc gtgcagaaca accagaccgc actgtcgact
    12121 ccggtggccg agcagagcgc ggtgcgtgtc tttgccgaca aggccgacgg ct
//
LOCUS       DASPGN010000086         4991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1654781, whole genome shotgun sequence.
ACCESSION   DASPGN010000086 DASPGN010000000
VERSION     DASPGN010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654781"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VD788_02405"
     CDS             complement(<1..765)
                     /locus_tag="VD788_02405"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXV75142.1"
                     /translation="MRTRSETRTVEMEIEIDAPPEAVWKALTDPDELVRWFPIEARVD
                     PGEGGNIFLGWGAGMEGNCRIERWQPNQRLTTGWVEGFGEGAAALDREPLVVDYRLDG
                     RGGSTVLRLVHSGFGAGEKWDEEYHGVSRGWQFELRSLRHYLERHAGRRRRVAWARKR
                     IELAPAAVWARLWSRDGLLREGRAPGAEEGQPFAFTMATDDRIEGHVLVHPAGREFGG
                     TVSNMDDAIFRTGYEYCGNQWEAMLWLSSWSASDEQV"
     gene            complement(797..1408)
                     /locus_tag="VD788_02410"
     CDS             complement(797..1408)
                     /locus_tag="VD788_02410"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV75143.1"
                     /translation="MRAERTELQVVDRELAAAALLHPLRRRIVEHLREPDSASGLARR
                     LGLPRQKLNYHLRELERAELLELVEERRRGNCTERVLRATAAAYLIGPRTLGRLAADP
                     ERIRDRFSSAYLMAVAARVIRDLADLRGRALDAGKRLATLTLQSEVRFASATDRAAFT
                     DELTREVARLAAKYHHADAPDGRTFRFVVGGYPEPDSGKETQN"
     gene            complement(1561..2919)
                     /locus_tag="VD788_02415"
     CDS             complement(1561..2919)
                     /locus_tag="VD788_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75144.1"
                     /translation="MRIGLVALAPLASLVLTPAVPAGEAPYPPSQVISGIVWDLTHTQ
                     DAYESDGWAVTWADDDRLYTAYSDGWGFHYPPDCPQESSKKSTAWAVIDGEATAYTAC
                     NIASNNETVGDGPTGKKAHGLLSIDGVLYLGMRNANQAGEECEIWMSTDKAVNWTDAG
                     WNFTELGLCALLNDGQDYDGAKDGYVYLYSPDSPSGYNEADSVVLLRAPKDQLMIEAA
                     WRYFAGLDAGEPLWSADPADRVPVFEFVGGCNRVHVVYYAPLRRYLMTMRARNRLSAH
                     PNHFSIYDAPKPWGPWTTVYYTESALPGSDMSPIDKSRGAWGENNHIPLKWVSDDGRR
                     FYMIYSGDDRFTVRGATIEVRLVTELRFADESMADWNAADGESVTYDVVRSGDATDFL
                     TGDASCVATDTPDTWATDTELPAPGLPFFYLVRAMRAGGSDPGPLGPDGDGNPRQARS
                     CP"
     gene            complement(3008..3083)
                     /locus_tag="VD788_02420"
     tRNA            complement(3008..3083)
                     /locus_tag="VD788_02420"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3048..3050),aa:Lys,seq:ttt)
     gene            complement(3157..4467)
                     /locus_tag="VD788_02425"
     CDS             complement(3157..4467)
                     /locus_tag="VD788_02425"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXV75145.1"
                     /translation="MPNVAIVGAQWGDEGKGKIVDLLSDRFDVVARFQGGPNAGHTVT
                     IGAKRHALHHVPSGVFRPEARIVIGNGTVIDLAKLIEEIDGLAGAGIDLAGRLFISDR
                     AHVILPMLKRIDAIDEAEAGEAGRIGTTLRGIGPTYQAKAARWGIRLADLADAGALLE
                     RVRSVLAGPTGERIRAAGERTDSAESVASEAHRQYRTLEPFVADTTTLLNDWLDQGLD
                     VLFEGAQGTMLDLDHGSYPFVTSSNTMAGALCAGLGVAPTRVTGTIGVFKAYATRVGS
                     GPMPTELGDAIGDRIRERGREFGTTTGRPRRCGWFDGVAARYAARINRFDGACISLLD
                     VLDDFDEVRICVAYELGGERLGCLPASAARAASVRPVYETMPGWRSDTTGIRRWDRLP
                     RAALAYLDRLSEIIGSEVVLVGVGPEREQSIVKPGGWLERQLAS"
     gene            4597..4791
                     /locus_tag="VD788_02430"
     CDS             4597..4791
                     /locus_tag="VD788_02430"
                     /inference="COORDINATES: protein motif:HMM:NF021366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HXV75146.1"
                     /translation="MSSWTLFLAGIGLAMLIEGTPYFVAPRAMRRLLRAMEGMSDGMM
                     RAAGFTLIVCGLLVTYVATR"
     gene            4800..>4991
                     /locus_tag="VD788_02435"
     CDS             4800..>4991
                     /locus_tag="VD788_02435"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HXV75147.1"
                     /translation="MAGRGTSAVRLDGSSLTLEQVGLVAAGAPCRLDDGARRRVRASR
                     AVVERAVAGGGQIYGVNTGF"
BASE COUNT          723 a         1781 c         1686 g          801 t
ORIGIN      
        1 aacttgctcg tcgctcgccg accagctcga aagccagagc atcgcctccc actggttgcc
       61 gcagtactcg tagccggtgc ggaagatggc gtcgtccatg ttcgacacgg tcccgccgaa
      121 ctctctcccg gccggatgga ccagcacgtg accttcgatc cgatcgtcgg tcgccatggt
      181 gaaggcgaac ggctgccctt cctccgcgcc gggcgctcgg ccctcccgca gcagaccgtc
      241 gcgactccag aggcgcgccc aaaccgcggc cggcgcgagc tcgatccgct tgcgggccca
      301 ggccacgcgt cgtcgccggc cggcgtgtcg ctcgagatag tgacgcagcg agcgcagctc
      361 gaactgccag ccgcgcgaga caccgtggta ctcctcgtcc catttctcgc cggcgccgaa
      421 accggagtga accaggcgca gcacggtcga accgccgcgc ccgtcgagac ggtagtcgac
      481 gacgagcggc tcgcgatcga gcgccgcggc gccctcgccg aatccttcga cccagccggt
      541 ggtcagacgc tggttgggct gccagcgctc gatccggcag ttgccctcca tcccggcgcc
      601 ccagccgagg aagatgttac cgccctcgcc cgggtcgacg cgtgcctcga tcggaaacca
      661 ccggaccagc tcgtcgggat cggtcaacgc cttccacacc gcctcgggcg gggcgtcgat
      721 ttcgatctcc atctcgaccg tgcgtgtctc ggacctcgtt ctcatcctct cgcctccttc
      781 ctttccctct gctttgtcag ttctgcgttt ccttgccaga gtcgggctcg ggataaccgc
      841 cgacgacgaa acggaacgtg cgtccgtcgg gcgcatcggc gtggtggtac ttggccgcca
      901 gtcgcgcgac ctcgcgcgtc agctcgtcgg tgaaggccgc gcgatcggtc gcgctggcga
      961 aacgcacctc cgactggagc gtgagcgtcg ccagacgctt gccggcgtcg agcgcccggc
     1021 cacgcaagtc agcgaggtcg cggatcacgc gcgcggcaac agccatcaga taggccgacg
     1081 agaagcggtc gcggatccgc tccgggtcgg ccgcgagccg gccgagcgtg cgcgggccga
     1141 tcaggtaggc cgcggccgtc gcccgcaaca cgcgttcggt gcagttgccg cgccgccgct
     1201 cctccaccag ctcgagcagc tcggcccgct cgagctcgcg caggtggtag ttcagcttct
     1261 gccgcgggag ccccaggcgg cgcgccagac ccgaggccga atccggttcc ctcaggtgct
     1321 cgacgatccg tcgtcgcagc ggatgcagca aggccgccgc ggccagctcc cgatcgacga
     1381 cctgcaattc cgtgcgttcg gctctcatcc cacttctcct tggattcacg atcccaagga
     1441 gaatacgacc gacaaatata tttgtcaaga tagattttta tttcggaaca agccgcccac
     1501 tcggacggac ccgctcgggt tcctcccgat tggacagcgc ggcgggacgg gtcggacggg
     1561 ctaggggcag ctacgggctt ggcgcgggtt gccgtcgccg tcgggcccga gcggccccgg
     1621 gtcgctcccg ccggcccgca tcgcccgcac cagatagaaa aacggcagac ccggggcggg
     1681 aagctcggtg tcggtcgccc aggtgtccgg cgtgtccgtc gcgacgcacg atgcgtcgcc
     1741 cgtcaggaag tcggtcgcgt cgcccgagcg aaccacgtcg taggtcaccg attcgccgtc
     1801 cgcggcgttc cagtcggcca tcgactcgtc ggcgaagcga agctccgtca ccagtcgcac
     1861 ctcgatcgtc gctccgcgga ccgtgaagcg atcgtcaccc gagtagatca tgtagaaccg
     1921 ccggccgtcg tccgagaccc acttgagcgg aatgtggttg ttctcgcccc acgcgccccg
     1981 gctcttgtcg atcggcgaca tgtccgagcc gggcagcgcg ctctcggtgt agtagacggt
     2041 cgtccacggc ccccacggct tcggtgcgtc gtagatgctg aagtggttgg ggtgggcgct
     2101 cagccgattc cgtgcgcgca tcgtcatcag gtagcgtcgg agcggcgcgt agtagaccac
     2161 gtggacgcgg ttgcagccgc cgacgaactc gaagaccggc acccggtcgg ccggatcggc
     2221 gctccacagc ggttcacccg cgtcgaggcc ggcgaagtac cgccaagccg cctcgatcat
     2281 cagctggtcc ttcggcgccc gcaacaagac gaccgaatcc gcctcgttgt agccgctcgg
     2341 actgtccggc gagtagaggt agacgtagcc gtccttcgcg ccgtcgtagt cttggccgtc
     2401 gttgagcaag gcgcagaggc ccagctcggt gaagttccag ccggcgtcgg tccagttcac
     2461 cgccttgtcg gtgctcatcc agatctcgca ctcttcgccg gcctggttcg cgttcctcat
     2521 cccgaggtag agcacgccgt cgatgctgag cagtccgtgg gccttcttgc cggtcggtcc
     2581 gtcgcccacg gtctcgttgt tggacgcgat gttgcacgcg gtgtatgcag tggcctcgcc
     2641 gtcgatcacg gcccacgcag tgctcttctt cgagctttcc tgggggcagt cgggcggata
     2701 gtggaagccc cagccgtcgg aatacgcggt atacagccgg tcgtcgtcgg cccaggtgac
     2761 ggcccagccg tcgctttcgt aggcgtcctg cgtgtgggtc aaatcccaga cgatcccgct
     2821 gatcacctgg ctcggcggat acggtgcctc cccggccggc accgctgggg tcaacaccag
     2881 cgatgcgagc ggtgcgaggg ccacgagccc gatcctcatt cgacctttcc gtcgagcata
     2941 cgaggtcata atcttacggt cgggcgggcc gaccgccacc ccggccgagg cgcgctcgtc
     3001 ttgttcgtgg tgagccgtgc cggattcgaa ccggcgaccc tcagcttaaa aggctggtgc
     3061 tctaccgact gagctaacgg cccatcgacg gggccgacat gctatcccgc cgccatgcga
     3121 cccgcaaccc gcacggccgc cgggccggcg cccgcatcag ctcgcgagct gccgctcgag
     3181 ccagcctccc ggcttgacga tcgactgttc gcgctcgggg ccgacgccga ccagaacgac
     3241 ctcgctaccg atgatctcgg acagacggtc gaggtaggcc agcgccgctc gcggcaggcg
     3301 atcccagcgg cggatcccgg tcgtgtcgct gcgccagccg ggcatcgtct cgtagaccgg
     3361 ccgaacgctc gcggcgcgcg ccgccgacgc gggcaggcag ccgagacgct cgccgccgag
     3421 ctcgtaggcc acgcagatcc gtacctcgtc gaagtcgtcg agcacgtcga gcagcgagat
     3481 gcaggcgccg tcgaagcggt tgatccgcgc ggcgtagcgg gccgcgacgc cgtcgaacca
     3541 gccgcaccgc cgcgggcggc cggtcgtcgt gccgaactcg cggccgcgct cacggatgcg
     3601 gtcgccgatc gcgtccccga gctcggtcgg catcggcccc gaaccgaccc gcgtggcgta
     3661 ggccttgaac acgccgatcg tgccggtgac gcgcgtcggc gccacgccca gccccgcgca
     3721 cagcgcgccg gccatcgtgt tggacgaggt gacgaacggg tacgatccgt ggtcgaggtc
     3781 gagcatcgtc ccctgcgccc cctcgaacag cacgtcgagc ccctggtcga gccagtcgtt
     3841 gagcagcgtc gtggtgtcgg cgacgaacgg ctcgagcgtg cggtactggc ggtgcgcctc
     3901 gctcgccacg ctctcggcgg aatcggtccg ctcgccggcg gcgcgaatcc gttcgcccgt
     3961 cggaccggcg agcaccgacc gcacccgctc gagcaacgca ccggcgtcgg ccaggtcggc
     4021 cagccggatg ccccagcggg cggccttggc ctggtaggtc ggaccgatcc cgcgcagcgt
     4081 cgtgccgatg cggcccgcct cgcccgcctc ggcttcgtcg atcgcgtcga tgcgcttgag
     4141 catcggcagg atcacgtgcg cgcgatcgct gatgaacaga cggcccgcga gatcgatgcc
     4201 ggctccggcc agaccgtcga tctcctcgat cagcttcgcc aggtcgatca ccgtgccgtt
     4261 gccgatgacg atccgggcct cgggccggaa gacgccggac gggacgtggt gcagcgcgtg
     4321 gcgcttcgcg ccgatcgtca cggtgtgccc ggcgttgggg ccgccctgga accgcgcgac
     4381 cacgtcgaag cggtccgaca gcagatcgac gatcttgccc ttgccctcgt cgccccattg
     4441 ggcgccgacg atcgcgacgt tgggcatctc tcctggggtc ccttcccgcg agtgacgcgc
     4501 cgcaggatgc ggtcgcgcgc ggtcgaaacg gcggaaagta taacacgcgc gacgcgcgcg
     4561 gccgggtcgc ggcgccgtgt gctagcatcg cccgccgtga gcagttggac cctgtttctc
     4621 gccggaatcg gcctggccat gttgatcgag ggcacgccct atttcgtcgc accgcgggcg
     4681 atgcgacgcc ttctccgagc gatggaaggg atgagcgacg ggatgatgcg ggcggccggc
     4741 ttcacgttga tcgtctgcgg attgctcgtg acctacgtcg cgacgcgctg aaggctcgca
     4801 tggcgggacg tgggacctcg gcggtgcggc tcgacggcag ctcgttgacg ctcgagcagg
     4861 tgggcctggt ggccgccggc gcgccctgcc gcttggacga cggggcgcgg cgcagggtgc
     4921 gggcctcgcg cgccgtcgtc gaacgcgcgg tcgccggcgg cggtcagatc tacggcgtca
     4981 acaccggctt c
//
LOCUS       DASPGN010000087         6961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_15392, whole genome shotgun sequence.
ACCESSION   DASPGN010000087 DASPGN010000000
VERSION     DASPGN010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6961
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15392"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VD788_02440"
     CDS             <1..728
                     /locus_tag="VD788_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXV75148.1"
                     /translation="KTVYLNIPLTEQELERATVETVAANGMREAYIRLVVTRGVGDLG
                     IDPQKCKVPTVVIIVSTISLYPKKYYDEGIPLVTASTRRIPMECLDPRIKSCNYLNNI
                     LAKLEAQRAGVPEAIMLNHYGRVAECTADNIFIVTDGRLRTPRLTEGALPGVTRAAVL
                     DLAREAGILTRETVLGLHDLYNAEECFLTGTGAEIVPVISIDGRQIGDGRPGAMTAKL
                     SAGFRELRVRAGHKVDFEAVPAN"
     gene            complement(741..1592)
                     /locus_tag="VD788_02445"
     CDS             complement(741..1592)
                     /locus_tag="VD788_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HXV75149.1"
                     /translation="MADSGDRDWTGRRAEVRVLVLTGLGLNCEAETELGFRLAGATTE
                     RVHLLDLLDAGRGKRLVDFQLLAFVGGFAFGDHLGAGFVLANKIRWRLYDELLRFIES
                     GGLAIGICNGFQTMVRLGLLPGLDGDYRTPCAALVPNERLGYRDAWVRLGFDPSSPCV
                     WTTGLTTMEMPARHGEGKFVTASPALLERLEDGGQIAARYVDDDGRPTERWPDNPNGS
                     PRAVAGICDPSGRIFGLMPHPDAYLYPFHHPNWLRRAREGTLPAEGEGLAIFRNGVDA
                     AARALVG"
     gene            complement(1585..4608)
                     /locus_tag="VD788_02450"
     CDS             complement(1585..4608)
                     /locus_tag="VD788_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HXV75150.1"
                     /translation="MVTRIEIAPRPDARDPLGEQLCRRITGLLGIGVEWVRTTEVYRV
                     DARLTADEAARVLGEFVNPVSQTGALGRCEQPPFDFIVAVGYKPGVTDPVGKSARVAI
                     EDTLGRPLGRGAAVYGSRLYALRGADRAQAERIATELLANPVIQTFEIRSFDEWRSSP
                     VDLSVPRVAGHPVPPVATVDLSGSDEELLRISRDGLLALDLDEMRAIRDHFARAGDDE
                     RRTRLGLGGSPTDVELECIAQTWSEHCKHKIFNATIRYTEPGQPEVELRSLFDSFIRR
                     TTETIDERLRATGRSWLVSVFHDNAGVVAFDERLHLVFKVETHNSPSALDPYGGAITG
                     IVGVNRDPFGTGIGAELLVNVWGYCFADPAYDGELPGGLLHPRRIRDGVHQGVIDGGN
                     QSGVPYGRGWELFDLRYLGKPLVFCGTVGALPVTVAGRPGEQKRARPGDLIVMTGGRI
                     GADGIHGATFSSAALDESSPVQAVQIGDPITQKMMFDFLLEARGLGLYNAITDNGAGG
                     LSSSVGEMARGAGGARLDLARAPLKYQGLAPWEILVSEAQERMTLAVPPDRIDRFIEL
                     ARRREVEATVLGEFTDDGFFHLLYGDRTVARLDMTFLHGGVAMRLEARWSPPIFDEPE
                     PSLRADESRLNETLTAMLGRLNLCSGEAAARHYDHEVKGLSVIKPFVGVGRDVPADAT
                     VFLAHHGSRAGFVLSEGVNPYLSEIDTEQMALYVVDEAVRKQLCAGARFDRIALLDNF
                     CWPDPVRSRATPDGEYKLAQLVRACRGLARACLSYGTPLISGKDSMKNDSTKGGVKIS
                     VPPTLLVSAIGRIDDVGRARTLEPRAAGDAIFLLGRTADECGGSEYFRHLGERAGLVA
                     TVGRPQPYVGNRLPRVDPEALRPLYLAFDEAARDGLIESATAPAKGGLALALARCAMS
                     TELGLRVDLGDAARDGALAADALLFSESGGRFVVTTRPETAAAFERRFAGLPCRRIGR
                     VTSEPGLLVTAGARPWLQSSRGELARVFKERWADG"
     gene            4686..5612
                     /locus_tag="VD788_02455"
     CDS             4686..5612
                     /locus_tag="VD788_02455"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXV75151.1"
                     /translation="MSDDTSPPLERGSVPRADVDQLLAFVAVARTGSVGRAAEELSRT
                     QPTISARLGGLEKLWGTRLFRRRARGMSLTPEGARLLPRAESILRELRELDRLAGVPL
                     ATADQLRVGAGDALARERLPRALAALLAERPAIEIQLREGTGPGLLDALRNGEIDLAL
                     VVGATGQSAGDGIDSLPLIRSDVVLLAPAASRRVPTRRRPTLEALARSRLVSLQRGSG
                     FRAHLERAFDDAGLSYRPAVEVGNLSLVRRFVAAGLGVALVPSVAFGEAGSRQGVALE
                     PTRDIPPLTYQHAVRRGVPLSAAARLLELLVA"
     gene            5669..>6961
                     /locus_tag="VD788_02460"
     CDS             5669..>6961
                     /locus_tag="VD788_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75152.1"
                     /translation="MTNGPRPRRAIVALLTLALCVAPARLAAGEAEADVPRTSIERWL
                     VAGPAAHPLPIFADAERGGVEVSALLDQPMLNSTRIRPAEGQTLVWVDGKRLSFKPTK
                     AGRQGRVDLPRPRAAGEPAVAWLAVHLRAARFAKLDLVLTGEHPRRVWLDGEPLASGE
                     GETEVESKIELIRGSHTLLVETILDPAREADWKIGVDLVGDDTAALVATTDPRRELDL
                     LDLLEAPEIGSIALSPDGTRIVASVSRVVPGTDESESWIEIRSAADGALEDSWRGQVQ
                     VRSVDWSPDGRFISYVTKAVDGGEDASTLFLYDVERRSAAPLLQSVEHLGRYLWSPTG
                     GAIVFATTHKADKDERGVKLLGGLLDRWADYRDKQFLHLVTVPDGSRRLLTAGDTTTD
                     PLDISPDGRRLLFTRRLEDLADRPFTRTELWQLDLRSFT"
BASE COUNT         1057 a         2490 c         2423 g          991 t
ORIGIN      
        1 ccaagacggt ctacctgaac atcccgctga ccgagcagga gctggagcgg gcgacggtcg
       61 agacggtcgc ggccaacggc atgcgcgagg cctacatccg gctggtggtg acacggggag
      121 tcggcgatct ggggatcgac ccgcagaagt gcaaggtgcc gacggtcgtc atcatcgtct
      181 cgacgatctc gctctatccg aagaagtact acgacgaggg aatcccgctg gtcacggcca
      241 gcacgcggcg gatcccgatg gaatgcctcg acccgcgcat caagtcgtgc aactacctga
      301 acaacatcct ggccaagctc gaggcacagc gcgcgggcgt cccggaagcg atcatgctca
      361 accactacgg ccgtgtcgcg gaatgcaccg ccgacaacat cttcatcgtc accgacggtc
      421 ggttgcggac gccgcggctg accgagggcg ctctgcccgg cgtcacgcgc gcggcggtgc
      481 tcgacttggc gcgcgaggcg ggaatcctga cgcgcgagac ggtgctcggg ctgcacgatc
      541 tgtacaacgc cgaggagtgc ttcctgaccg ggaccggggc cgagatcgta ccggtgatct
      601 cgatcgacgg ccgacagatc ggcgacggcc gtccgggagc gatgacggcc aagctgtccg
      661 ccggcttccg cgagctgcgg gtgcgtgccg ggcacaaggt cgacttcgag gcggttccgg
      721 cgaactgacg gtcggcggcg ctagccgacg agggctcggg cggcggcgtc gacgccgttg
      781 cggaagatcg cgagtccctc gccctcggcc ggcagcgtcc cctcgcgtgc ccggcgcagc
      841 cagttcgggt ggtggaacgg gtagaggtag gcgtcggggt ggggcatcag gccgaagatc
      901 cgacccgacg gatcgcagat cccggcgacg gcgcgcggcg acccgttcgg gttgtccggc
      961 cagcgctcgg tcggccgacc gtcgtcgtcg acgtagcgcg cggcgatctg cccgccgtcc
     1021 tcgaggcgct cgagcagcgc cggcgaggcg gtgacgaact tgccctcacc gtgccgcgcc
     1081 ggcatctcca tcgtggtcag cccggtggtc cagacgcacg gcgagctcgg gtcgaagccg
     1141 agccggaccc aggcgtcccg ataacccaac cgctcgttcg gcacgagcgc cgcgcacggc
     1201 gtgcgatagt cgccgtcgag gccgggcagc agccccagcc gcaccatcgt ctggaagccg
     1261 ttgcagatgc cgatggccag accgcccgat tcgatgaagc gcagcagctc gtcgtacaac
     1321 cgccagcgga tcttgttggc cagcacgaaa ccggcgccga gatgatcgcc gaaggcgaag
     1381 ccaccgacga aggccagcag ctgaaagtcg acgagacgct tgccgcgccc cgcatcgagc
     1441 agatcgagca ggtgcacgcg ctcggtcgtc gccccggcca ggcggaagcc gagctcggtc
     1501 tcggcctcgc agttcagccc gagtccggtg aggaccagca cccgcacctc ggcgcgtctc
     1561 ccggtccagt cccgatcacc cgaatcagcc atcggcccac ctctccttga aaacccgcgc
     1621 gagctcgccg cggctcgact gcagccacgg ccgcgcgccg gccgtcacca acagaccggg
     1681 ctcgctcgtc acgcggccga tcctgcgaca cggcagaccg gcgaaccgtc gctcgaaggc
     1741 ggccgccgtc tcggggcgcg tcgtgacgac gaagcggccg ccggactcgg agaacagcag
     1801 ggcgtcggcc gcgagtgccc catcgcgcgc cgcgtcgccg agatcgacgc gcaggccgag
     1861 ctcggtcgac atcgcacagc gggcgagcgc cagtgcgagc ccacccttgg ccggcgccgt
     1921 cgccgactcg atcaggccgt cgcgggcggc ctcgtcgaac gcgaggtaga gcggccgcaa
     1981 cgcctcggga tcgacgcgcg ggaggcggtt gccgacgtac ggctggggcc ggccgacggt
     2041 cgccaccagg cccgcgcgct cgccgaggtg gcggaagtac tcgctcccac cacactcgtc
     2101 cgcggtccgg ccgagcagga agatcgcgtc ccccgccgcg cggggctcga gcgtcctcgc
     2161 gcggcccacg tcatcgatcc gtccgatcgc ggaaaccagc agggtcggcg ggacgctgat
     2221 cttgactccg cccttggtcg agtcgttctt catcgagtcc ttgccggaga tcaacggggt
     2281 gccgtagctc aggcaggccc gcgccagccc gcggcaggcg cggaccagct gcgcgagctt
     2341 gtactcgccg tcgggcgtgg ctcgcgaccg cacggggtcg ggccagcaga agttgtcgag
     2401 cagcgcgatc cgatcgaaac gcgcgcccgc gcagagctgc ttgcgcacgg cctcgtcgac
     2461 cacgtacagc gccatctgtt ccgtgtcgat ctccgacagg taagggttca cgccctcgga
     2521 aagcacgaaa cccgctcgcg agccgtgatg cgcgaggaag accgtggcgt cggccggaac
     2581 gtcccgcccg acgccgacga acggcttgat cacgctcaga cccttgacct cgtgatcgta
     2641 gtgtcgcgcc gcagcctcgc ccgagcacag attcaatcga ccgagcatcg cggtcaacgt
     2701 ctcgttcaac cgggactcgt cggcccgcag cgacggttcg ggctcgtcga agatcggcgg
     2761 cgaccagcgc gcctcgagac gcatcgcgac tccgccgtgg aggaacgtca tgtcgagccg
     2821 cgccacggtt cgatcgccgt acagcaggtg gaagaaaccg tcgtcggtga actcgccgag
     2881 caccgtcgcc tcgacctcgc ggcgccgcgc cagctcgatg aagcggtcga tcctgtcggg
     2941 cggcaccgcg agcgtcatcc gttcctgggc ctccgagacc aggatctccc acggcgccag
     3001 cccctggtac ttgagcggag cgcgggccag gtcgagccgc gcgccaccgg cgccccgcgc
     3061 catctcaccg accgacgagg acagcccgcc ggcgccgttg tcggtgatcg cgttgtacag
     3121 acccagcccg cgcgcctcga gcaggaagtc gaacatcatc ttctgagtga tcggatcgcc
     3181 gatctgcacc gcctgcaccg gggaagactc gtcgagcgcg gcggaggaga aggtcgcgcc
     3241 gtggatgccg tcggcgccga tccggccgcc ggtcatcacg atcaggtcgc ccggccgggc
     3301 ccgcttctgt tctccggggc gccccgcgac cgtgaccgga agcgcgccga ccgtgccgca
     3361 gaagacgagc ggcttgccca ggtagcgtag gtcgaacagc tcccagccgc ggccgtaggg
     3421 aacgccgctc tggttgcccc cgtcgatcac gccctgatgc acgccgtcgc ggatccgtcg
     3481 cggatggagc aacccgccgg gcagctcccc gtcgtaggcc gggtcggcga aacagtagcc
     3541 ccagacgttg accaacagct cggcgccgat gcccgtgccg aacgggtcgc gattgacgcc
     3601 gacgatgccg gtgatcgccc cgccgtacgg atcgagtgcc gaaggcgagt tgtgcgtttc
     3661 gaccttgaac acgaggtgca gccgctcgtc gaaggcgacg acccccgcgt tgtcgtgaaa
     3721 caccgacacc aaccaggacc ggccggtcgc gcgcaggcgc tcgtcgatcg tttcggtcgt
     3781 gcggcggatg aagctgtcga acaacgatcg aagctcgacc tcgggttgcc ccggttcggt
     3841 gtaacggatg gtggcgttga agatcttgtg cttgcagtgt tcgctccagg tctgggcgat
     3901 gcattcgagc tcgacgtcgg tcggactacc gccgagtccg agccgggtgc ggcgctcgtc
     3961 gtcgccggcc cgcgcgaagt ggtcgcggat cgcgcgcatc tcgtcgagat cgagcgccag
     4021 cagaccgtcg cggctgatgc gcagcaactc ctcgtcgctg cccgacaggt cgacggtcgc
     4081 gacggggggt accggatggc cggcgacacg cggcacgctc agatcgacgg gcgacgaccg
     4141 ccactcgtcg aacgagcgga tctcgaaggt ctggatcacc gggttggcca acagctcggt
     4201 cgcgatccgc tcggcctgcg cacggtcggc gccgcgcagg gcgtacagcc gcgaaccata
     4261 gacggccgcc ccccgcccca gcggtcggcc gagcgtgtcc tcgatcgcca cgcgcgcgct
     4321 cttgccgacg gggtcggtca caccgggctt gtagccgacg gcgacgatga agtcgaacgg
     4381 cggctgctca cagcgcccga gcgcgccggt ctgcgacacc gggttgacga actcgcccag
     4441 cacgcgtgca gcctcgtccg cggtcagccg cgcgtccacg cggtagacct cggtcgtgcg
     4501 gacccactcg acgccgatgc cgagcagccc ggtgatccgc cggcagagct gctcgcccag
     4561 cgggtcgcgc gcgtccgggc gcggggcgat ctcgatgcga gtcaccatct cgaggtcctc
     4621 cgatccctac tgtagagcgt ctggtgctat cgaactagtc gatttcttat atactaggca
     4681 tcgaaatgtc agatgataca tcgcccccgc tcgagcgcgg gtccgtcccg cgtgccgacg
     4741 tcgaccagct gctcgccttc gtcgcggtgg cgcggaccgg aagcgtcggg cgcgcggccg
     4801 aggagctttc gcgaacccag ccgacgatca gcgcccggtt gggcggtctc gagaagctgt
     4861 ggggaacccg tctgttccgc cgccgggcac gcggcatgtc gctgacgccc gagggcgcgc
     4921 ggctattgcc tcgtgccgag tcgatcctgc gcgagctgcg ggaactcgac cgtcttgccg
     4981 gagtcccgct cgccaccgcc gaccagctcc gcgtcggcgc cggggacgcg ctcgcgcgcg
     5041 aacggctgcc ccgggcgctc gccgccttgc tcgccgagcg gcccgcgatc gagatccagc
     5101 tgcgcgaggg caccggtccc gggctgctcg acgcactgcg caacggcgaa atcgacctgg
     5161 cgctcgtcgt cggcgcgacc ggccagagcg cgggcgacgg catcgacagc ttgccgttga
     5221 ttcgctccga cgtggtgctg ctggcgcccg cggcctcgcg gcgcgtgccg acccgacggc
     5281 gaccgacgct cgaagcgctc gctcgctcgc gcctggtctc gctgcaacgc ggctccggct
     5341 tccgggccca cctcgaacgg gcgttcgacg acgccgggct gtcctatcgg ccggccgtcg
     5401 aagtcggaaa tctctcgttg gtgcgtcgct tcgtcgccgc cggcctgggc gtcgccctgg
     5461 tgccgtcggt cgcgttcggc gaggccggct cgcgccaggg ggtcgcgctc gaaccgacgc
     5521 gcgacatccc gccgttgacc taccagcacg cggtccgccg cggcgtgccg ttgtcggcgg
     5581 cggcgcgctt gctcgagctg ctcgtcgcct gaatcggcgc cgcccggtgg tatgctcccc
     5641 gtccgattcc agccgaacgg agtttccgat gacgaacgga ccgaggccga ggcgcgcgat
     5701 cgtcgcgctg ctgacactgg cgttgtgcgt ggcgccggca cgactggccg ccggcgaggc
     5761 cgaagccgac gtaccgcgca cgtcgatcga acgttggctc gtcgcgggac ccgcggccca
     5821 cccgcttccg atcttcgccg atgccgagcg gggcggggtc gaggtctcgg cgctgctcga
     5881 tcaaccgatg ctgaacagca cccgcatccg gccggccgag ggacagacgc tggtctgggt
     5941 cgacggtaag cggctgagct tcaagccgac gaaggccggc cggcagggcc gggtcgacct
     6001 gccacgaccc cgcgccgccg gcgagccggc ggtcgcctgg ctcgcggttc atctgcgggc
     6061 ggcgcggttc gccaagctcg atctcgtgct gaccggcgag cacccgcgac gcgtctggct
     6121 cgacggcgaa ccgctggcgt cgggcgaggg cgagaccgag gtcgaatcga agatcgagct
     6181 gatccgcggc agccatacgc tgctggtcga gaccatcctc gaccccgcgc gcgaggccga
     6241 ctggaagatc ggcgtcgatc tcgtcggcga cgacaccgca gcgctcgtcg cgacgaccga
     6301 tccccgacgc gagctcgatc tgctcgatct gctcgaagcg ccggagatcg gctcgatcgc
     6361 gctttctccg gacggcacgc ggatcgtcgc ttcggtgtcg cgcgtggttc ccggaacgga
     6421 cgagagcgag agctggatcg agatccgctc ggccgccgac ggggcgctcg aggacagttg
     6481 gcgcggacag gtgcaggtgc gcagcgtcga ctggagcccc gacggccgtt tcatctcgta
     6541 cgtgacgaag gcggtcgacg gcggcgaaga cgcgtcgacc ctgtttctct acgacgtcga
     6601 gcgacgatcg gccgccccgt tgctgcaatc cgtcgagcac ctcggccgct acctctggtc
     6661 gccgaccggc ggcgcgatcg tcttcgccac cacccacaag gccgacaagg acgagcgcgg
     6721 cgtcaagctg ctcggcgggc tgctcgaccg ttgggccgat tatcgggaca agcagttcct
     6781 ccacctcgtc accgtgcccg acggcagccg ccgcctgttg acggccggcg atacgacgac
     6841 cgacccgctc gacatctcgc ccgacggccg gcggttgctg ttcacgcgcc ggctcgagga
     6901 cctcgccgac cgaccgttca cgcggaccga gctgtggcag ctcgatctgc ggagcttcac
     6961 c
//
LOCUS       DASPGN010000088         7283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1186331, whole genome shotgun sequence.
ACCESSION   DASPGN010000088 DASPGN010000000
VERSION     DASPGN010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186331"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VD788_02465"
     CDS             <1..527
                     /locus_tag="VD788_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75153.1"
                     /translation="QSWLEIEVTDDAGRSIYSSGRRDEKNFLEPGTFLFKVEPVDQYG
                     NLIDRHNLWEMVGVRFRRALFPGYSDSVDFSVSCPSELAPAGTDRDATRSSRRSERDY
                     VLSAGTETGRYRVTAALNYRKVDQFLLNYMFGEQAGLTAPVVEIDRATAEIAVRADPA
                     EPHGTEQSATRAGG"
     gene            535..3054
                     /locus_tag="VD788_02470"
     CDS             535..3054
                     /locus_tag="VD788_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXV75154.1"
                     /translation="MGPGRAWGRRRRRLFWTALSLAGGAAFAAAAGYALRRTAATAEY
                     RPGEQIAEITSQLEQGIPEEAPRARFADVTTSAGLGGFRSFRGGRTSQLPEDMGAGAA
                     WGDYDGDGDDDLFVVSNGGPLTAVESARARSRLYRNDGRGRFVEEPAFPETRIVGMAA
                     AWGDHDADGDLDLALTGYHALRLYRNDGGRFARDPAVEDRAGFWAGASWADFDLDGDL
                     DLYVCGYVKYVKDARGRERTTQQYGRSVPYTLNPASFEPEPNLLFRNDGPAGFHEVAG
                     SLGADNPAGRSLGALWHDFDDDGRPDLYIANDISDNALLLNRAEGFVDSSHASWVADY
                     RGAMGLAVGDWNRDGDDDLFVTHWVAQENALYDSLRVDMAARGQHGGLRFVDVADQLG
                     LGQIALQRVGWGAEFFDLDSDGWLDLAVANGSTFETAERPPELRVEPPFLFWNRRGQS
                     FHDLAPTIAALARPRVGRGLAISDYDDDGDQDLLQVDRDGGVRLLRNDTEQSHRIGFR
                     LLDPTGAPLEMTRVELHLAQRVVQRTLGGVSYLSQSSRVLHFGLGRDEAIERIVVVWP
                     DRERTVYHGAGVDARFELVRGESRPRRVAEWSRPAGEAPPSRERLAEFWRTQRAAMDA
                     MKVDGEPRRSIELFRRALELNPDHADSLYYLANCLAETGDVDGALEQLDRLIRADPQS
                     HRAHKRWGTLRASTAADDTQLEHAESALLRAARINPEETGVSLVLGELHLLRGDRRAA
                     QQRLEWVVRTNPQSGEAFFVLGYLAWAAGDDALARRQLMRARATRGNDTKPRGFAAEG
                     DTRRKMHDETSLFEPLYRAWDGETDPAASFAALTDRLARRL"
     gene            3320..3700
                     /locus_tag="VD788_02475"
     CDS             3320..3700
                     /locus_tag="VD788_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75155.1"
                     /translation="MNVGGALSALARSPDRGFAVVVMRKRRDRLTAAPVLGAAADEEA
                     EEVALLIGAFSDEEDALDGAVAVGDVLDALGEIATHGGAWPVFSAEAGGGAAAEGGSL
                     VGFSIDDEVEIFAFLEGPKSVWDD"
     gene            3841..4590
                     /locus_tag="VD788_02480"
     CDS             3841..4590
                     /locus_tag="VD788_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75156.1"
                     /translation="MPRARWALVCVVSSLAGAVAGPIVVAPAWRQATTTVSGDARGAL
                     GVELGVLAGTIAAKAAGGVYEYDRSRRHERTETLATDIAGSSGSGAEAGETVGDVGYR
                     HFADLLGDWKLEIWATTSTDRRISATGSARASPAGDRAVRIRYGGIEAEGLDRSIDGY
                     TLLSYLPGRGFDLENSFDTLDRPRRFVGEYLVNQNAYTFYPIDDRSATIVRSRVRVDL
                     RISSPKLWVVETYTLIDGKEVQVQSYRFSRT"
     gene            complement(4614..6740)
                     /locus_tag="VD788_02485"
     CDS             complement(4614..6740)
                     /locus_tag="VD788_02485"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXV75157.1"
                     /translation="MACLALIAGCSKDAEEANVEQEDILLAEWSGPHGGVPAFDAMDL
                     AVLEPALEAGMAMQLEEIDAIAADPAPPDFDNTIVAMERTGRPLSRAMTYMGIWRSNM
                     STPEFREIQQRMAPKLAEFRTKITQNRALFERIRSVYESDELRSLRPDQQRLVKLIYE
                     EFARNGATLEGVAKERYGEINKRLAELHTQFADNVLADEESYVVFLTQEQLGGLPESF
                     VKAAAAAAAERGRDGEYAITNTRSSIDPFLTFSDERGLRELVWRNYYNRGDNGDDHDN
                     NAVIAEILALRNERVRLLGYDNYAEWRLEDRMAQHPDRALGLMEAVWPAAIARVEQEV
                     ADMQAIADREGAGITIEPWDYRYYAEKVRRARYDLDSDEVKQYLQLDKLRQAMFFVAG
                     ELFGFEFTPVPAGEVPVYHEDVTVWEVTDKTTGEHVGLWYLDPYARQGKRSGAWATSF
                     RSHETFDGKQTVLASNNSNFIKGAPGEPVLISWDDATTLFHEFGHALHSLSSKVAYPT
                     LNGGVRDYTEFQSQLLERWVLTDPVIDNYLVHHETGEPIPAELVAKIKNASTFNQGFS
                     TTEYLGSALVDMRYHTVDPTGLDADAFERETLAALSMPGEVVMRHRSPHFSHIFSGEG
                     YSAGYYGYMWADVLTADAAEAFRQAPGGFYDKQLADKLVEYLFAARNSVDPAEAYRAF
                     RGRDARIEALMRDRGFPVIGSAAVAD"
     gene            6933..>7283
                     /locus_tag="VD788_02490"
     CDS             6933..>7283
                     /locus_tag="VD788_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75158.1"
                     /translation="MRPLKPGPSFALLAGLAIATSSFATEDDEIYSCADDSGTIVIQT
                     DPCPAPALAPPAAPPPPPKAAPPKPATERPRGEPPRVTTLRTRHTTWQVVPRSERPAP
                     PPRREFGRLRFETSA"
BASE COUNT         1094 a         2462 c         2548 g         1179 t
ORIGIN      
        1 tccagagctg gctcgagatc gaggtcaccg acgacgcggg gcggtcgatc tactcctcgg
       61 gccggcggga cgagaagaac ttcttggagc ccggcacgtt cctgttcaag gtcgagccgg
      121 tcgaccagta cggcaacttg atcgaccgcc acaacctgtg ggagatggtc ggtgtgcgat
      181 tccgccgggc gctgttcccg ggctattcgg attcggtcga cttcagcgtg tcctgtccgt
      241 ccgagctcgc gccggccggc accgatcgcg acgcgacccg atcgagccgg cgcagcgagc
      301 gcgactacgt gctgtcggcc ggcaccgaga ccggccgtta ccgggtgacg gcggcgctga
      361 actatcggaa ggtcgatcag ttcctgctga actacatgtt cggcgagcag gcgggcctga
      421 ccgcgcccgt cgtcgagatc gaccgggcga cggccgagat cgcggtgcga gcggaccctg
      481 ccgagccgca cgggaccgag cagtccgcga ctcgcgccgg gggttgagcg ctcgatggga
      541 ccgggccgcg cctggggtcg gcgacggcgg cgcctgttct ggaccgcgct atcgttggcc
      601 ggcggagccg ccttcgctgc cgccgccgga tatgcgctgc gtcgcaccgc ggcgacggcc
      661 gagtatcgcc ccggcgagca gatcgccgag atcacgagcc agctcgagca gggaatcccc
      721 gaggaggctc cgcgagcgcg gttcgccgat gtgacgacgt ccgctggcct cggcgggttc
      781 cgcagcttcc gtggcggccg aacgagtcag ctcccggagg acatgggtgc cggcgcggct
      841 tggggcgact acgacggcga cggggacgac gacctgttcg tggtgagcaa cggcggcccg
      901 ctcacggcgg tcgagtccgc gcgtgcccgc tcgcgtctgt atcgcaacga cgggcgtggc
      961 cggttcgtcg aggaacccgc tttccccgag acccggatcg tcgggatggc cgcggcatgg
     1021 ggcgaccacg acgccgacgg ggacctcgac ctggcgctga ccggttacca cgcactgcgc
     1081 ctgtaccgca acgacggcgg tcgcttcgcc agggacccgg ctgtcgagga tcgtgccggt
     1141 ttttgggccg gggcgtcgtg ggccgatttc gatctcgacg gcgacctcga cctgtacgtc
     1201 tgcggctacg tgaagtacgt caaggacgcc cgcggccgtg agcggacgac gcagcagtac
     1261 ggccgaagcg tgccctacac gctcaacccg gcgtcgttcg agcccgagcc caatctgctg
     1321 tttcggaacg acgggccggc gggctttcac gaggtcgcag ggtcgctcgg cgccgacaac
     1381 cccgccgggc gcagcctggg cgcgctgtgg cacgatttcg acgacgacgg acgaccggac
     1441 ctctacatcg ccaacgacat ctcggacaac gcgttgttgc tcaaccgcgc ggaggggttc
     1501 gtcgattcga gtcacgcatc gtgggtcgcc gactatcgcg gtgcgatggg actcgccgtc
     1561 ggcgactgga atcgcgacgg cgacgacgat ctgttcgtga cccactgggt cgcgcaggag
     1621 aacgcgctgt acgattcgtt gagggtcgac atggccgcac gcggtcaaca cggcggactg
     1681 cgctttgtcg acgtcgccga ccaactcggc ctcgggcaga tcgcgttgca gcgggtcggc
     1741 tggggcgcgg agttcttcga cctcgactcg gacggttggc tcgacctcgc cgtcgccaac
     1801 ggctccacgt tcgagaccgc ggaacggccg cccgagctgc gcgtggagcc gccgttcctg
     1861 ttctggaatc gccgcggaca gtcgttccac gacctggcgc cgacgatcgc ggcgttggcc
     1921 cgaccgcgag tcggccgcgg gctcgcgatc tcggactacg acgacgacgg cgatcaggac
     1981 ttgctgcagg tcgaccgcga cggtggtgtg cggttgctgc gcaacgacac cgagcaaagt
     2041 caccggatcg gattccggct gctcgatccg accggtgcgc cgctcgagat gacgcgggtc
     2101 gagctgcacc tggcgcagcg ggtcgtgcag cggacgctcg gcggagtctc ctacctgtcg
     2161 cagagcagcc gcgtgttgca cttcgggctg ggccgggacg aggcgatcga gcggatcgtc
     2221 gtcgtctggc ccgaccggga gcggaccgtc taccacggcg ccggcgtcga cgcacgcttc
     2281 gagctcgtgc gtggcgagtc gcgcccgcgg cgagtcgccg agtggagtcg acccgcgggc
     2341 gaagcgccac cctcgcgcga gcggctggcc gagttctggc gcacccaacg cgcggcgatg
     2401 gacgcgatga aggtcgacgg tgagccgcgg cgatcgatcg agctgttccg gcgcgcgctc
     2461 gaactgaacc cggatcacgc tgactcgttg tactacctcg ccaactgctt ggccgagacc
     2521 ggcgacgtcg acggggcgct cgagcagttg gaccggctga ttcgtgccga tccgcagagt
     2581 caccgagccc acaagcgatg gggaacgctg cgcgcgtcga ccgcagcaga cgatacacag
     2641 ctcgagcacg ccgaatcggc gctgctacgg gcagcccgga tcaatccgga ggagaccggc
     2701 gtctcgctcg tgttgggcga gcttcacctg ctgcgcggcg accggcgcgc cgcgcagcaa
     2761 cgactggagt gggtcgtgcg aaccaacccg caatcgggcg aggcgttctt cgttctcggg
     2821 tacctggcct gggccgcagg cgacgatgcg ctcgcgcgcc ggcagctcat gcgcgcgcgc
     2881 gccacccgcg gcaacgacac caagccgcgg ggcttcgccg ccgagggcga cacccggcgc
     2941 aagatgcacg acgagacgag tctgttcgaa ccgctctacc gcgcgtggga cggcgagacg
     3001 gatccggcgg ccagttttgc cgcgttgact gatcgactgg cgcgacggct gtgagccggg
     3061 tgagaactcg agagtcgagc cgacggtgtc ctgcatcccg gcatcatctt gcccgaccgg
     3121 tagttgatct ccttcggcgc cccggccgcg accggcccga gggcgatcga gacgacgatc
     3181 acggtcgcga cgcagagtgg cagacatcga atcaggcgat acgcccggcg ctcggtgagt
     3241 cggcctcatc gtacacggaa tcgtgcgtcc cgaacaaacg cgccgggcgg tagaatcggc
     3301 cacgcggcgg gaggtaggaa tgaacgtggg tggtgcgctg tcggcgctcg ctcgcagccc
     3361 cgatcgaggc tttgccgtgg tcgtgatgcg caagcggcgc gatcgactga cggccgcgcc
     3421 ggtgctgggc gcggcggccg acgaggaggc cgaggaggtg gcgctcttga tcggcgcctt
     3481 ctccgacgag gaagatgcgc tcgacggtgc ggtcgcagtc ggcgacgtgc tggacgcgtt
     3541 gggcgaaatc gcgacccacg gcggcgcgtg gccggtgttc tccgccgagg ccggcggcgg
     3601 ggccgcggcc gaaggcggat cgctggtcgg cttctcgatc gacgacgagg tcgagatctt
     3661 cgccttcctc gagggcccga aatcggtgtg ggacgactga cgccgcggct ttgccgcagt
     3721 tttgggcccg gatcggccgt ttccgcgggc tcggccggcg tggcgggacc ggcgtggtag
     3781 cattcggtca cggctccccg ctcccgcggg cccgtcttgt aggggatctt gacagtgagg
     3841 atgccacgcg cacgctgggc gctcgtctgc gtcgtgtcgt cgctcgccgg cgccgtcgcc
     3901 ggtccgatcg tggtggcgcc cgcttggcgg caagcgacga cgacggtgag cggcgacgcc
     3961 cgtggcgcct tgggggtcga gctcggcgtg ttggccggga cgatcgcggc caaggcggcc
     4021 ggcggtgtct acgaatacga tcgctcgcgg cggcacgagc gtacggagac gttggcgacg
     4081 gacatcgccg gctcgagcgg atcgggcgcc gaggcgggtg agacggtcgg cgacgtcggc
     4141 taccgccact tcgccgacct gctcggtgac tggaagctcg agatctgggc gacgacgtcg
     4201 accgatcggc gtatctcggc taccggcagc gccagagcgt cgcctgccgg tgaccgggcc
     4261 gttcgcatcc gctacggcgg gatcgaggcc gaagggctcg accgctcgat cgacggatac
     4321 acgttgctga gctacctccc cggccgcggc ttcgatctcg agaacagctt cgacacgctg
     4381 gaccgcccac gccgattcgt cggcgagtac ctggtcaacc agaacgcata caccttttat
     4441 ccgatcgacg atcgatcggc gacgatcgtg cgctcgcggg tgcgcgtcga cttgcggatc
     4501 tcgagcccca agttgtgggt cgtcgagacc tacacgctga tcgacggcaa agaggtccag
     4561 gtccagtcct accgcttcag ccgcacgtga gccggcaccg cccctctcgg cggtcagtcg
     4621 gcaacggccg ccgatccgat caccggaaag ccacgatcgc gcatcagcgc ctcgatgcgg
     4681 gcgtcgcgcc cgcggaacgc tcggtaggcc tcggccggat cgaccgagtt gcgtgcggcg
     4741 aacaggtatt cgaccagctt gtccgccagc tgcttgtcgt agaacccgcc gggtgcctgc
     4801 cggaacgcct cggcggcgtc ggcggtcaac acgtcggccc acatgtagcc gtagtaaccc
     4861 gccgagtagc cctcgccgga gaagatgtgg ctgaaatgcg ggctgcggtg gcgcatcacg
     4921 acttctcccg gcatgctcag cgccgccagg gtttcgcgct cgaaggcgtc cgcgtcgaga
     4981 ccggtcgggt cgaccgtgtg gtaacgcatg tcgaccaacg cggagccgag gtactcggtc
     5041 gtcgagaagc cttggttgaa ggtggacgcg ttcttgatct tggccacgag ctcggcgggg
     5101 atcggctcgc cggtctcgtg gtgcaccagg tagttgtcga tcaccggatc ggtcaacacc
     5161 cagcgttcga gtagctgaga ctggaactcg gtgtagtcgc gcacgccgcc gttgagcgtc
     5221 gggtaggcga ccttggacga caacgagtgc aatgcatgtc cgaactcgtg gaacaacgtg
     5281 gtggcgtcgt cccatgagat cagcaccggc tctccgggcg cgcccttgat gaagttggag
     5341 ttgttcgaag ccagcaccgt ttgcttgccg tcgaacgtct cgtggctgcg gaagctcgtg
     5401 gcccaggcgc ccgatcgctt gccttgccgc gcgtaggggt cgaggtacca cagaccgacg
     5461 tgctcgcccg tcgtcttgtc ggtcacctcc cagacggtga cgtcctcgtg gtagaccgga
     5521 acctcgccgg cgggcaccgg cgtgaactcg aagccgaaca gctcgccggc gacgaagaac
     5581 atcgcctggc gcagcttgtc gagttgcagg tactgcttca cctcgtccga gtccagatcg
     5641 tatctcgcgc ggcgcacctt ctcggcgtag tagcgatagt cccagggctc gatcgtgatc
     5701 cccgcgcctt ctcggtcggc gatcgcctgc atgtcggcga cctcctgttc gactcgcgcg
     5761 atcgccgccg gccagacggc ctccatcaac ccgagcgcgc gatcggggtg ctgcgccatg
     5821 cggtcctcca accgccactc ggcgtagttg tcgtagccca gcagccggac acgctcgttg
     5881 cgcagcgcga ggatctcggc gatcacggcg ttgttgtcgt ggtcgtcgcc gttgtcgccg
     5941 cggttgtagt agttgcgcca gacgagctcg cgcagcccgc gctcgtcgga gaaggtgagg
     6001 aacggatcga tcgacgaccg cgtattggta atcgcgtact ctccgtcgcg gccacgctcg
     6061 gcagccgccg ccgcggcggc cttgacgaac gactcgggca gaccgccgag ctgctcctgg
     6121 gtcaggaaga ctacgtagct ttcctcgtcg gccagcacgt tgtcggcgaa ttgcgtgtgc
     6181 agctcggcca gtcgcttgtt gatctcgccg tagcgctcct tggccacgcc ctccagcgtc
     6241 gcgccgttgc gcgcgaactc ctcgtagatc aacttgacca gccgctgttg atccggcctc
     6301 agcgaccgca gctcgtcgct ctcgtagact gagcggatac gctcgaacaa cgcccggttc
     6361 tgcgtgatct tggtgcggaa ctcggccagc ttcggcgcca tccgctgttg gatctcgcgg
     6421 aactcgggcg tcgacatgtt cgagcgccag atgcccatgt acgtcatcgc gcgcgagagg
     6481 gggcggccgg tacgctccat cgcgacgatc gtgttgtcga agtcgggcgg cgccggatcg
     6541 gcggcaatcg cgtcgatctc ctcgagttgc attgccatgc cggcctcgag cgctggctcc
     6601 agcacggcga ggtccatcgc gtcgaaggcc gggacgccgc cgtgcggtcc gctccactcc
     6661 gccagcagga tgtcttcctg ctccacgttc gcttcctcgg cgtccttcga gcaccccgcg
     6721 atcaaggcga ggcaggccac tatcagcaat gtaggcttcg acaacgtgcg gtgcatcgtt
     6781 caaccccctg tttcgccgaa cgattctagc gatcccgcac ggtcaaggca cggacctgcg
     6841 gaaacccccg acttcggcgc agtccctcgc ccgcgaccgg tgggcccttg ccgtcagcgg
     6901 ccgcctccct acatttggca gaaaggattc cgatgcgtcc tctcaagccg ggtccgtcgt
     6961 tcgcgctgct cgccgggttg gcgatcgcga cctcgtcgtt cgcgaccgag gacgacgaga
     7021 tctactcgtg cgccgacgac agcgggacga tcgtgatcca gaccgatccg tgcccggcgc
     7081 ccgccctggc gccgccggcc gcgccaccgc ccccgcccaa ggcggcgccg ccgaagcccg
     7141 cgaccgaacg accccgcggc gaaccgccac gcgtcacgac gttgcgcaca cgccacacca
     7201 cctggcaggt cgtcccgcgc tcggagcgac cggccccgcc gccccggcgc gagttcggcc
     7261 gactccgctt cgagacgtcg gcc
//
LOCUS       DASPGN010000089         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1654992, whole genome shotgun sequence.
ACCESSION   DASPGN010000089 DASPGN010000000
VERSION     DASPGN010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1654992"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VD788_02495"
     CDS             complement(<1..213)
                     /locus_tag="VD788_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75159.1"
                     /translation="MSPRRIRFDIRHARKRLAVVLVAWATAAVAFYLLAVRPNVRQYE
                     ALQQGTAPQQTRMAEMQAEVDEREAYL"
     gene            complement(210..1043)
                     /locus_tag="VD788_02500"
     CDS             complement(210..1043)
                     /locus_tag="VD788_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75160.1"
                     /translation="MQKIDLNLSGNPFRNNVVLWIGFGTATAVLFGFTLFSVTTYVEY
                     GSRLTELRNTVGNFEHRRLDLETRGTGALKAIKQIDIDALELQAEMANMVIDRKAFSW
                     TKLFNSIEDVLPQRVRMTSVRPIFNAARAGEDPTSRAARAGMPVAIEGVAATYLEFTE
                     LERALQDDGHFGRVLPQRLSKTESNEILFQISFDYYAAGDATVVADEPESGEATIAGT
                     AADETHEAGVDEPVRDGAAIETTADGEADELDGPSRARDGTPPADVEPPAGADGTGGD
                     A"
     gene            complement(1043..2059)
                     /gene="pilM"
                     /locus_tag="VD788_02505"
     CDS             complement(1043..2059)
                     /gene="pilM"
                     /locus_tag="VD788_02505"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HXV75161.1"
                     /translation="MNSLSQIIERVRGLQLDALLGLRPPYPPVALEVDRGQVTLVRLK
                     LRRRAKPLLEAHAVRSLPEEAIPASIFQPAGGTIEGIATPFRELFERSGTRPGRVSLI
                     LPDNLAKVSLIHLPERPASARQLDELVRARMRRAVPFRLEEAVISYQVIPGAGRQIAV
                     LVLLARKALIERYEHALESIGARVGLVDVSTPNLINLCHKRLGETSRGGKDAALLNCA
                     ANYFSLVIVRDERVIFFRCKTFAPGTTSSHDANGFLAREVSGSLSYYREKLAGRKIET
                     LLVRSVQAPFEEVSAKLDELELATIEPISPADEVDLPPGTRFETSVGVRLAPAIGAAV
                     SRGR"
     gene            complement(2082..3713)
                     /locus_tag="VD788_02510"
     CDS             complement(2082..3713)
                     /locus_tag="VD788_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXV75162.1"
                     /translation="MADAADPAVEAAGAEQVLSEELQAQNLAQRLDLEYVDLDNFEID
                     PELFRSIPVDLMFRYNFVPRHRTQTGLSVVVADPTDVLMIDELELLLGTSISVCVGTQ
                     TAIQEILKKSESSQRVLEEATEEFRIQIVTEDDETGEETLNIDRLTSDSSPIIKLVDS
                     TIFNALQRRASDIHVETRDREVVIKYRIDGVLYQAMEPIDKKFHSTIISRLKVMSELD
                     ISEKRIPQDGRFKLRVKGRTVDFRVSILPTVHGEDCVIRVLDKESMSEEFKNLNLDVC
                     GFDARDIRRLRRFIKEPYGMVLVTGPTGSGKTTTLYAAVSEIRNEEDKIITIEDPVEY
                     QLPGITQIPVNEKKGLTFARGLRSILRHDPDKVMVGEIRDQETAQIAIQSALTGHLVF
                     TTVHANNVVDVIGRFLNMNVEPYNFVSALNCILAQRLVRLICESCRRPVKATREQLHE
                     SSLDPERYGEYTFHEGRGCIDCNGTGYRGRTAIAELLDMSDRIRDMILQRRPGSEIKR
                     AAKEEGMTFLREAALSKVFGGKSTLHEINKVTFVD"
     gene            complement(3747..>4420)
                     /locus_tag="VD788_02515"
     CDS             complement(3747..>4420)
                     /locus_tag="VD788_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXV75163.1"
                     /translation="LVALMVFFIIPKFNEFLQDFGTELPLITQVIVGVAMFCKDNWQF
                     IVVATVGGAVGFMMWNRTANGRYLVDAFKLRIPLIGAIVRNYAQNRFTRTLATLQSGG
                     IPLVTSLELSIGAVGNTVFERELRQVTVKVREGRSLWESLDATGLISDINLQMIRVGE
                     STGSLVEMLEASSEFTDEEIDTQLARLVTLIEPAMLVFMALIVATMLLSIYLPMIQLY
                     GASGV"
BASE COUNT          747 a         1516 c         1365 g          792 t
ORIGIN      
        1 cagataggct tcacgctcgt cgacctcggc ctgcatctcg gccatgcgtg tctgctgcgg
       61 tgcggtgccc tgctgcagcg cctcgtactg ccggacgttc ggacgcacgg ccagcaggta
      121 gaacgcgacg gcggcggtgg cccaggcgac gagcacgacc gcgagccgct tgcgcgcgtg
      181 gcgaatgtcg aagcggatgc ggcgcgggct catgcgtcgc ctcccgtccc gtccgcaccg
      241 gcaggcggct cgacgtctgc cggcggcgtc ccgtctcgcg cccgggacgg cccatcgagc
      301 tcgtccgctt caccgtcggc cgtcgtctcg atcgccgcgc cgtcccgaac cggctcgtcg
      361 actcccgcct cgtgcgtctc gtcggcggcg gtccccgcga tcgtcgcctc tcccgattcc
      421 ggctcgtcgg cgaccacggt cgcgtcgccc gcggcgtagt agtcgaagct gatctggaac
      481 aggatctcgt tgctctcggt cttgctcaag cgctgcggca gcacccggcc gaagtgaccg
      541 tcgtcctgta gcgcgcgctc gagctcggtg aattcgaggt aggtcgcggc cacaccctcg
      601 atcgcgaccg gcatgcctgc cctggccgcg cgcgaggtcg gatcctcccc cgcacgcgcg
      661 gcgttgaaga tcggacgcac gctcgtcatc cgcacgcgct gcggcagcac gtcctcgatc
      721 gagttgaaca gcttggtcca ggaaaacgcc ttgcgatcga tcaccatgtt ggccatctcg
      781 gcctgcagct ccagcgcgtc gatgtcgatc tgtttgatcg ccttcaacgc ccccgtgccg
      841 cgcgtctcga gatcgagccg acggtgctcg aagttcccca cggtgttgcg cagctccgtc
      901 agccgcgagc cgtactcgac gtatgtcgtc acgctgaaca gggtgaaacc gaacagcacc
      961 gccgtcgccg taccgaatcc gatccacaac acgacgttgt tccggaaggg attgccggac
     1021 aggttgaggt cgatcttctg catcaacgac cccgcgacac ggcggcgccg atcgccggcg
     1081 cgagccgcac accgacactc gtctcgaaac gcgtgccggg tggaaggtcg acctcgtccg
     1141 ccggcgagat cggctcgatc gtggcaagct ccagctcatc gagcttggcc gacacttcct
     1201 cgaacggcgc ctgcacgctc cgcacgagca gcgtctcgat cttccgtccc gcgagcttct
     1261 cgcgatagta ggacagcgag cccgacacct cgcgcgccag gaagccgttg gcatcgtggc
     1321 tcgaagtcgt accaggggcg aacgtcttgc agcggaagaa gatcacgcgc tcgtcgcgaa
     1381 cgatcaccag cgaaaagtag ttcgccgcgc aattgagcag cgccgcgtcc ttgccgcccc
     1441 gcgacgtttc gccgagccgc ttgtgacaca ggttgatcag gttcggcgtg ctgacgtcga
     1501 ccagaccgac acgcgcgccg atcgattcga gcgcgtgctc gtaacgctcg atcagcgcct
     1561 tgcgcgcgag cagcaccagc accgcgatct gccgtcccgc gcccggtatc acctggtagg
     1621 agatcacggc ctcctcgaga cggaacggca ccgcgcgccg catgcgcgcc cgcaccagtt
     1681 cgtcgagctg gcgggcggag gcggggcgct ccggcagatg gatcagactg accttcgcca
     1741 ggttgtcggg gaggatcagc gacaccctgc cgggccgcgt gccactgcgt tcgaacagct
     1801 cgcggaacgg cgtcgcgatc ccctcgatcg tcccgccggc cggttgaaag atcgaggccg
     1861 gaatcgcttc ctcgggcaac gaccggaccg cgtgggcctc gagcagcggc ttggcccgcc
     1921 gccggagctt cagccgtacg agcgtgacct gcccgcggtc gacctcgagc gcgaccggcg
     1981 gatacggcgg gcgcagcccg agcagcgcgt cgagctggag cccgcgcacg cgctcgatga
     2041 tctgcgacaa cgaattcatc cgctgcaact ccctcttccg ctcaatcgac gaacgtcacc
     2101 ttgttgatct cgtgcagcgt cgatttgccg ccgaagacct tcgacaacgc tgcctcgcgc
     2161 aggaaggtca tgccctcttc cttggcggct cgcttgatct cggagcccgg ccggcgctgc
     2221 aggatcatgt cacgaatgcg atcggacata tcgagcagct ccgcgatcgc cgtccgcccg
     2281 cggtagccgg tgccgttgca atcgatgcag ccgcgtccct cgtggaacgt gtactcgccg
     2341 tagcgctcgg gatcgaggct cgattcatgc aactgctcgc gcgtcgcctt gaccgggcgc
     2401 ctgcacgact cacagatcag ccggaccaac cgctgggcca ggatgcagtt gagcgccgag
     2461 acgaagttgt acggctccac gttcatgttg agaaagcgac cgatcacgtc gacgacgttg
     2521 ttggcgtgga cggtggtgaa caccaggtga ccggtcagcg ccgactggat cgcgatctgc
     2581 gccgtctcct ggtcgcgaat ctcgccgacc atcaccttgt ccgggtcgtg acgcaggatc
     2641 gagcgcagcc cgcgggcgaa ggtcaacccc ttcttctcgt tgaccggtat ctgggtgatg
     2701 ccgggcagct ggtactcgac cgggtcttcg atcgtgatga tcttgtcttc ctcgttgcga
     2761 atctcggaga ccgccgcata cagcgtggtg gtcttgcccg atccggtggg cccggtcacc
     2821 agcaccatgc cgtacggctc tttgatgaag cgccggagcc gcctgatgtc acgcgcgtcg
     2881 aagccgcaga cgtcgaggtt caggttcttg aactcctcgc tcatcgactc cttgtccagc
     2941 acgcggatca cgcagtcctc gccgtgaacc gtcggcagga tcgagacgcg gaaatcgacc
     3001 gttcgcccct tgacccgcag cttgaaccgg ccgtcctgcg ggatacgctt ctccgagatg
     3061 tcgagctcgg acatcacctt caagcgcgag atgatcgtgc tgtggaactt cttgtcgatc
     3121 ggctccatcg cctggtacag cacgccgtcg atgcgatact tgatcaccac ctcccgatcg
     3181 cgggtctcga cgtggatgtc cgacgccctg cgctgcagcg cgttgaagat cgtcgagtcg
     3241 acgagcttga tgatcgggga cgaatccgag gtcaaccggt cgatgttgag cgtttcttcg
     3301 ccggtctcgt cgtcctcggt gacgatctgg atgcggaact cctcggtcgc ttcctccagc
     3361 acccgctggg acgactcgct cttcttcagg atctcctgga tcgccgtctg cgtgccgacg
     3421 cagaccgaga tgctcgtgcc cagcagcagc tcgagctcgt cgatcatcag cacgtcggtc
     3481 ggatcggcga ctaccaccga cagaccggtc tgcgtccgat ggcgtgggac gaagttgtag
     3541 cggaacatca ggtcgaccgg gatcgaacga aacagctcgg gatcgatctc gaagttgtcg
     3601 aggtcgacgt actccaggtc gagccgctgc gccaggttct gcgcctgcag ctcttccgac
     3661 agcacctgct cggcaccggc cgcctcgacc gcggggtcgg ccgcgtccgc caaccgcctt
     3721 tcatccgcca tgaccggttc cctgcctcat acgcccgacg caccgtagag ctgaatcatc
     3781 ggaaggtaga tcgacagcag catggtcgcc acgatcagtg ccatgaagac cagcatcgcc
     3841 ggctcgatca acgtcaccag tcgcgcgagc tgcgtgtcga tctcctcgtc ggtgaactcc
     3901 gacgaggcct ccagcatctc caccaacgat ccggtcgact cgccgacacg gatcatctgc
     3961 aggttgatgt cgctgatcag accggtcgcg tcgagcgact cccacaacga tcgaccttcg
     4021 cgcaccttga ccgtcacctg acgcagttcc cgctcgaaga ccgtgttgcc gaccgctccg
     4081 atcgacaact cgagcgaagt aaccagcggg atgccgccgg actggagcgt ggcgagcgtg
     4141 cgggtgaacc gattctgcgc gtagtttcgc acgatggcgc cgatcaacgg gatcctcagc
     4201 ttgaacgcgt ccaccagata tctaccgttc gcggtgcggt tccacatcat gaatccgacg
     4261 gcgccgccga ccgtggccac cacgatgaac tgccagttgt ctttgcagaa catggcgacg
     4321 ccgacgatga cctgtgtgat cagcggcagc tcggtaccga aatcctgcaa gaactcgttg
     4381 aatttcggga tgatgaagaa aaccatcaac gcgaccagcg
//
LOCUS       DASPGN010000090         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_15765, whole genome shotgun sequence.
ACCESSION   DASPGN010000090 DASPGN010000000
VERSION     DASPGN010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15765"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VD788_02520"
     CDS             complement(<1..609)
                     /locus_tag="VD788_02520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV75164.1"
                     /translation="MRSPRHPYLSVVVPLYDEQESIRLLYQRIRSACDRLERSYEIVF
                     VDDGSRDATYESMVEIQREDPAVTAVRFRKNFGQTAAMAAGFACSRGEVIVSMDGDLQ
                     NDPADIPILLDKIAAGYDVVCGWRKNRQDKLISRRVPSVIANWLIGRVTGVPIHDNGC
                     SLKAYRAALIKRISLYNEMHRFIPAMSTLAGARITEVVVNHHA"
     gene            complement(617..1513)
                     /locus_tag="VD788_02525"
     CDS             complement(617..1513)
                     /locus_tag="VD788_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR03006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HXV75165.1"
                     /translation="MGRQGDGVLNAMTVDVEDYFQVSAFEDVVDRSEWARLPSRVEAS
                     TARLLDLFDRFEVRATFFVLGWIGENHPALVREISARGHEVACHGYDHKLLYEQEPEA
                     FRRDTVKSREILQDLSGQPVVGYRAASFSIGRDNLWTLDVLAEAGFTYDSSVFPIVHD
                     RYGMPGAPRHIHRLKTPGGHSLVEVPPTTVRIGKLTLPAAGGGYLRLLPLGLTKWALS
                     RLNRHERQPAIVYIHPWEVDPGQPRIRTRLTTSLRHYTGLAGVERKLGALMQRYRFGP
                     LCEIVRQLAEQDERNEPAVLTR"
     gene            complement(1522..1761)
                     /locus_tag="VD788_02530"
     CDS             complement(1522..1761)
                     /locus_tag="VD788_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV75166.1"
                     /translation="MFISSKRRGASSSDSASGARREEILTSREAREFLKIGRTKLWEL
                     TRCQDIPAYRVGSGSRASLRYKRSELLAWLDQNRI"
     gene            complement(1811..>2267)
                     /locus_tag="VD788_02535"
     CDS             complement(1811..>2267)
                     /locus_tag="VD788_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75167.1"
                     /translation="GLSHLLAGQATDDHAILELEKSRLHVLPSGELCRDPLELLGSSL
                     AELIKSLRERYDCILIDTPPLVLFPDADAIGVHSDGALVVARVGHTDVSSYKLAVDAV
                     TSTRILGVILNDVSRRSFADWGKYRGGYANKDYYTSYYREGKDEGKDEE"
BASE COUNT          387 a          749 c          725 g          406 t
ORIGIN      
        1 cgcgtggtgg ttgacgacga cttcggtgat tctcgcgccg gccagcgtcg acatcgccgg
       61 gatgaagcgg tgcatctcgt tgtacagcga gatccgcttg atcagcgcgg cgcgataggc
      121 cttcagcgag cacccgttgt cgtgaatcgg cacgccggtg acccggccga tcaaccaatt
      181 cgcgatcacc gacggcaccc gacgcgaaat cagcttgtcc tgccggttct tgcgccagcc
      241 acagacgacg tcgtaacccg cggcgatctt gtcgagcagg atcgggatgt cggcggggtc
      301 gttctgcaag tcaccgtcca tgctgacgat gacctcgccg cgcgagcagg cgaacccggc
      361 cgccatcgcc gccgtctgcc cgaagttctt gcggaagcga accgcggtga cggccggatc
      421 ctcgcgttgg atctcgacca tcgactcgta ggtcgcgtcg cggctgccgt cgtcgacgaa
      481 gacgatctcg tacgaccgct cgagccggtc gcacgccgag cgaatccgtt ggtagagcag
      541 gcggatgctc tcctgctcgt cgtacagcgg tacgacgacg gacaaatacg gatgtcgcgg
      601 tgatctcatc aacgtcctat ctcgtcagca cggccggctc gtttcgttcg tcctgctcgg
      661 cgagctgccg cacgatctcg cagagcgggc cgaagcggta acgttgcatc aaggcgccga
      721 gcttgcgctc gacgccggcc agtcccgtgt agtgtcgcag cgacgtcgtc agacgggttc
      781 ggatcctcgg ctggccggga tcgacctccc acgggtggat gtagacgatc gccggctgcc
      841 gctcgtgccg gttgagtcgg ctcagcgccc acttcgtcag gcccaggggg agcagccgca
      901 gatagccgcc gccggccgcc ggtagggtca gcttgccgat tcggaccgtc gtcggcggga
      961 cctcgacgag cgagtggccg cccggggtct ttagacgatg gatgtgtcgc ggggccccgg
     1021 gcatgccgta gcggtcgtga acgatcggga acacgctcga atcgtaggtg aatccggctt
     1081 cggccaacac gtccagcgtc cagagattgt ccctgccgat cgagaacgaa gcggcacggt
     1141 agccgaccac cggctgtccg ctgagatcct gcaggatctc tcgcgacttg acggtatccc
     1201 ggcggaacgc ctcgggttct tgctcgtaca gcagcttgtg gtcgtagccg tggcaggcga
     1261 cctcgtgacc gcgtgccgag atctcccgga cgagcgccgg gtgattctcg ccgatccaac
     1321 cgaggacgaa gaaagtggcc cgcacctcga atcgatcgaa caggtcgagc agccgggcgg
     1381 tgctggcctc gacacgagag ggcaatcggg cccattcgct gcgatcgacg acgtcctcga
     1441 aggccgagac ctggaagtag tcttcgacgt cgaccgtcat cgcgttgagc acaccgtcgc
     1501 cctggcgacc cacaggcgac ctcagatgcg attctggtcg agccatgcga gcagttccga
     1561 ccgcttgtag cgcaggctgg cgcgactgcc gctgccgacg cggtaggccg gaatgtcctg
     1621 gcaacgggta agttcccaca gcttggtgcg gccgatcttg agaaactccc gagcctccct
     1681 gctcgtcagg atctcctctc tcctcgcgcc gctcgcggaa tcgctcgaac tcgcccccct
     1741 ccgcttgctc gaaatgaaca tcccccccat gtcctccgaa cgacggtagc gccgtgattg
     1801 agagctgaac tcactcttcg tctttccctt cgtctttccc ttcgcggtaa taggacgtgt
     1861 agtagtcttt gttggcgtaa ccgccgcggt acttgcccca gtcggcgaaa ctcctgcgag
     1921 aaacgtcgtt gaggatcacg cccagtatcc gtgtcgacgt gaccgcgtcg acggccagct
     1981 tgtagctcga cacgtcggtg tgccccacgc gcgccacgac cagcgccccg tcgctgtgaa
     2041 caccgatcgc atcggcatcg gggaacagga cgagcggcgg ggtgtcgatc aggatgcagt
     2101 cgtaacgctc gcgcagcgat ttgatcagct ccgccagcga cgagccgagc agctccaacg
     2161 gatcgcgaca cagctcgccc gacggcagca cgtgaagccg actcttctcc agttcgagga
     2221 tcgcgtggtc gtccgtcgcc tggccggcga gcaggtgcga cagaccg
//
LOCUS       DASPGN010000091         2588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1304208, whole genome shotgun sequence.
ACCESSION   DASPGN010000091 DASPGN010000000
VERSION     DASPGN010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304208"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VD788_02540"
     CDS             <1..1122
                     /locus_tag="VD788_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75168.1"
                     /translation="RLVGLLAGAAWATKYTALPACAGLALAAAWLREGRPRTRLAAAA
                     WVGAVTVCVGLLWNVRSWVLTGNPLYPAFYGLLGGRYWSETSAAAMAAEVSHGGFGDR
                     GPAAYLLALRDLILHSEGLGFPSGINVLYVLVALAGIPLFRRIRGGSALLLVAGVSYA
                     GWCTTSLNLRYALFMLAVLAPFAAAACDRGVERLGRVLRSPLAASVPCLVLAVAVAGP
                     MLLTIERHTRLYGDGTRLLGGTPRAELHATRLQLGLAGRELAAALPAEARILLIGEGR
                     VGLLPRPAAASSAYDEPDIARYLRDLASIREVNRRLGEFTHVVVNYRELERFNTRYGF
                     SGRFSPEAWKLFNDWLANGLIPVARYGNVVVYRIPDDER"
     gene            1125..2147
                     /locus_tag="VD788_02545"
     CDS             1125..2147
                     /locus_tag="VD788_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV75169.1"
                     /translation="MKSQDPVSVVDQLSSAGRSKPSSGRKLIIQIPCYNEEPTLARTL
                     AELPRNVPGFDRVELLIVDDGSSDHTSAVARRLGVDHVVRHNGNRGLAHAFMTGVRSA
                     VARGADVIVNTDADHQYRAADIPRLLRPILDGDADIVIGARPIDTIRHFSPIKRALQR
                     LGSRVVRALSHTEVDDAPSGFRAMTRDAALRLNVFTGYTYTLETIIQAGLSNLRVANV
                     PVAVNEPTRPSRLIRSTPQYVWRSIRTLFSAYLIYRPIRLFAGLSLLFLAPGFALALR
                     YLYFVSIGEGRGHVQSVIVGGALVVCGVFMLAIATVAHLLSINRKLLEELSYLERERA
                     GGTRDP"
     gene            2171..>2588
                     /locus_tag="VD788_02550"
     CDS             2171..>2588
                     /locus_tag="VD788_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75170.1"
                     /translation="MAHPTITSDESAGDPPKRILFVPVSGPSGIGEYQRSLFLAQSLA
                     ERHPQWDIRIVVSQDAPYVDSVPLPTVRTSRSPTRVPDELDRILERLRPDVVVFDSAG
                     RRRSLKRAKRLAARTVFVSTHARKRWKAFRLSRLRHT"
BASE COUNT          352 a          912 c          890 g          434 t
ORIGIN      
        1 cggctggtcg gcctgttggc cggcgccgcc tgggccacca agtacacggc gctgccggcc
       61 tgcgccggtc tggcactggc cgcggcctgg ctccgggaag ggcggccgag gacgcgactg
      121 gccgcggccg cgtgggtcgg cgcggtgacg gtctgtgtcg ggctgctgtg gaacgtccgc
      181 tcctgggtcc tgaccggtaa tccgctctac ccggcgttct acggtctgct gggagggcgc
      241 tactggagcg agaccagcgc ggcggcgatg gccgccgagg tctcgcacgg cggcttcggc
      301 gaccgcggcc cggcggccta cctgctcgcg cttcgcgatc tgatcctgca ctcggagggg
      361 ctgggcttcc ccagtggaat caacgtgctc tacgtgctgg tcgcactggc cggcatcccg
      421 ctcttccgcc gcatacgagg tggttcggcc ctgttgctcg tcgccggggt cagctacgcc
      481 ggttggtgta ccacgtcgct gaacctgcgc tacgcgctgt tcatgctcgc ggtgctggcg
      541 ccgttcgccg cggcggcgtg cgaccgggga gtcgagcggc tcgggcgggt gctgcgatcg
      601 ccgctggcgg cgagcgtacc gtgcctcgtg ctggcggtcg cggtcgccgg gccgatgctg
      661 ctgacgatcg aacggcacac caggctctac ggcgacggga cacggttgtt gggcggcacc
      721 ccgcgggccg agctccacgc gacgcggctc cagctcggac tcgccgggcg agagctggcc
      781 gctgcgctac ccgccgaggc gcgcatcctg ctgatcggcg aggggcgcgt cgggttgctg
      841 cccagaccgg ccgccgcctc ctcggcctac gacgagcccg acatcgcgcg ttacctgcga
      901 gatctggcct cgattcgcga ggtcaaccgg cggctcggcg agttcacgca cgtcgtcgtc
      961 aactaccgcg agctcgaacg gttcaacacg cgctacggct tttccgggcg gttctcaccc
     1021 gaggcctgga agctgttcaa cgattggctg gccaacgggc tgatcccggt cgcgcgctac
     1081 ggcaacgtcg tggtctatcg tatccccgac gacgagaggt gacgatgaag tcacaggacc
     1141 cggtctcggt ggtcgatcag ctcagctcgg ccggacgcag caagccgagt tccggccgca
     1201 agctgatcat ccagatcccg tgttacaacg aggaaccgac gctggcacgg accctcgcgg
     1261 agcttccgcg gaacgtcccc ggcttcgacc gggtcgagct gttgatcgtc gacgacggga
     1321 gctcggatca cacctccgcc gtcgcgcgcc ggctgggggt cgatcacgtc gtgcggcaca
     1381 acggcaaccg cgggctggcc cacgcgttca tgaccggcgt gcgctcggcg gtcgctcgcg
     1441 gcgccgacgt catcgtcaac accgacgcgg accaccagta ccgtgccgcc gatatcccgc
     1501 ggctgttgcg cccgatcctc gacggcgacg cggacatcgt catcggcgcg cgtccgatcg
     1561 acaccattcg ccacttctcg ccgatcaagc gcgcgctgca gcgactgggc agtcgggtcg
     1621 ttcgcgcgct cagtcacacc gaggtcgacg acgcgcccag cggctttcgc gcgatgacgc
     1681 gcgacgcggc gctcaggctg aacgtcttca ccggctacac ctacacgctg gagacgatca
     1741 tccaggcggg tctgtcgaac ctccgcgtcg ccaacgtccc ggtcgcggtc aacgagccga
     1801 cccggcccag ccggctgatc cgcagcacgc cgcagtacgt ctggcgcagc atccgcacgc
     1861 tgttcagcgc atacctgatc taccggccga ttcgtctgtt cgccgggctc tcgctgctgt
     1921 tcctggcgcc gggattcgcg ctggcgctgc gctatctcta cttcgtttcg atcggcgagg
     1981 gacgcgggca cgtgcaatcg gtcatcgtcg gcggggcact ggtcgtttgc ggggtgttca
     2041 tgctggcgat cgcgaccgtc gcgcacctgc tgtcgatcaa ccgcaaacta ctcgaggagc
     2101 tgagctatct cgaacgcgag cgggccggcg gcacgcgcga tccgtgagct cgcgtcctat
     2161 aatgccgccg gtggcccacc ccacgatcac gagcgacgag tcggcgggcg atccgccgaa
     2221 acgaatcctg ttcgtgccgg tctccggccc gtcggggatc ggcgagtacc agcgctcgct
     2281 gttcttggcc cagagcctgg ccgagcgtca cccgcagtgg gacatccgca tcgtcgtcag
     2341 ccaagacgcg ccgtatgtcg actcggttcc gctgccgacc gtgcgtacga gccgttcgcc
     2401 gacgcgagtt cccgacgagc tcgatcggat tctcgagcgg cttcgccccg acgtcgtcgt
     2461 cttcgactcg gcgggacggc ggcgcagcct gaagcgagcc aagcggctcg cggcccggac
     2521 ggtcttcgtc agcacgcacg cgcggaagcg gtggaaggcg tttcgtctgt cgcgcctgcg
     2581 tcacaccg
//
LOCUS       DASPGN010000092         4311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1304853, whole genome shotgun sequence.
ACCESSION   DASPGN010000092 DASPGN010000000
VERSION     DASPGN010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1304853"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="VD788_02555"
     CDS             <1..1317
                     /locus_tag="VD788_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXV75171.1"
                     /translation="VVLEHDGRPLSLDNDVVFSMIGREAPLEFFRRSGIRIAGEWTPG
                     RIAGLAAFLAFCFALYNWKSGGFLEHWHRAWGGFPFDLADRLAAAEPGSLLDVLRISA
                     SGPAFYYTLAYSALVAIFGVRRIRRRRTPYVTVQTVTLIAVQVVPLFLLPEILLPWLD
                     ARGWLPRWLADPLFPAVDYGHGREFWRAYGLILAWPLNVYNVFTDSPNFAWLVICFLQ
                     TFVAIPAMIYFWGKGAYCGWICSCGALAETLGDTHRHKMPHGPRFNRLNLAGQLILAV
                     AFVLLALRILSWTFPEADALRRFNSMALWQSNLGWKWVVDVLLAGIVGYGVYFWYSGR
                     VWCRFFCPLAALMHLYARFSRFRILADKKKCISCNVCTSVCHQGIDVMNFANKGLPMG
                     DPECVRCSACVGACPTGVLTFGQVGRDGRVLGTDRLAASAVRLSER"
     gene            complement(1323..3314)
                     /locus_tag="VD788_02560"
     CDS             complement(1323..3314)
                     /locus_tag="VD788_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV75172.1"
                     /translation="MNRRRLSRLVVLVDLLAVGSFALLFGRHGNPDWSGTFLLATLIA
                     LAGATPVRLPGMKTSVSATDPFLLTALAAFGPVSACIVASAGVVGSTVARESGRHVRH
                     IAFNLGNVVLSTALASAVYLALGGSPGAPTAAQVWPLLFAATVYFLLNTGLVTAAISI
                     DGRRPFLATWRESGLWMAVTTYGGLTLAVGLLHVLQVAGPSGLALGVPPCWLLAAFYR
                     THKERQEAQQQRIDRVEGLNQQLEDKVAERTSELQQALSGIEQVNTRLRQANERLTDA
                     NRAKSEFLANVSHELRTPLNAIIGFSGLLSDRKFGALGEQQREFVRDINESGEHLLRL
                     INGILDLSKIEAGKMEVHRERFDAAKLTSDTVAMVRHQAAVEGLELTVDDGRRSIAVN
                     LDPGMYRQVLLNLLSNAIKFTPAGGWVRVEISADGTDLSCRVIDSGIGIEAGDLERIF
                     TEFFQVDGSYSRSYEGTGLGLALVRKMVGMHGGSVEVRSTPGAGSTFSLRFPDAVVAA
                     AEPPPGADRRAPPPPASRGPATPPSDDQPPAILVIEDNPVNRKLARNVLRSRGYEVWE
                     AASGEEGLRVLLERRPALVLMDIQLPGMDGLETTRRIKRDPLTADLPVIALTAHAWEA
                     DEQRARDAGCSGYITKPIRLADFPAQIAAFLRPAPAEAG"
     gene            complement(3311..>4311)
                     /locus_tag="VD788_02565"
     CDS             complement(3311..>4311)
                     /locus_tag="VD788_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV75173.1"
                     /translation="LDVMMPQMTGHEVCRALKGDPATRLCQVMLVTALDRMPDQVTGL
                     DTGADDYLSKPVRREEFLAKVRALLRARHLLGELERARDELARRNEELQLKKTLAQTL
                     VHDLKSPLSAVLGNLDLLHARSDEALHHMIDRSKQGARRMLRMILDLLDVEALEEGRI
                     RPSIERVELTELARASADEAATPAEQRQIEIVMRVDGPVWATADPLLTRRVIDNLLSN
                     AMSHSPTGGFIELQVGYREEGAEIVVSDQGPGVPEQLREHVFDKYAQVRSSEPATATT
                     NRGLGLTFCRLVVEAQGGSIWIESSPAGGACFRTVLPAAARLADELPVDLAAGRRQ"
BASE COUNT          604 a         1528 c         1447 g          732 t
ORIGIN      
        1 gtcgtgctcg agcacgacgg ccggccgctg agcttggaca acgacgtcgt cttctcgatg
       61 atcggccgcg aagcgccgct cgagttcttc cgtcgctccg gaatccgcat cgccggtgaa
      121 tggacgcccg ggcgcatcgc cggcctggcc gcgttcctcg ccttctgctt cgcgctttac
      181 aactggaaat cgggcgggtt cctcgagcat tggcatcgcg cgtggggcgg tttcccgttc
      241 gatctcgccg accggctggc cgcggccgag cccggctcgt tgctcgacgt gctgcggatc
      301 tccgccagcg gtccggcgtt ctactacacg ctggcctatt cggcgctggt cgcgatcttc
      361 ggcgtgcggc ggatccgtcg ccggcgcacg ccgtacgtca ccgtgcagac cgtcacgctg
      421 atcgcggtgc aggtcgtccc gctgttcctg ctccccgaga tcctgctgcc ctggctcgac
      481 gcccgcggct ggctcccccg ctggttggcc gacccgttgt tccccgccgt cgactacggc
      541 cacggccgcg agttctggcg cgcctacggg ctgatcctgg cctggccgct caacgtctac
      601 aacgtcttca ccgactcgcc caacttcgcc tggctcgtca tctgcttcct gcagacgttc
      661 gtcgcgatcc cggcgatgat ctacttctgg ggcaagggcg cctactgcgg ctggatctgc
      721 tcgtgcggcg cgctggcgga gacgctcggc gacacccaca ggcacaagat gccgcacggc
      781 ccgcgcttta accggttgaa cctggccggc cagctgattc tcgccgtcgc gttcgtcttg
      841 ctcgcgttgc gcattctgtc gtggaccttc cccgaggccg acgcgctccg gcggttcaac
      901 tcgatggcgc tgtggcaatc gaatctcggc tggaagtggg tcgtcgacgt gttgttggcc
      961 ggcatcgtcg gctacggcgt ctacttctgg tactcgggtc gcgtgtggtg tcgcttcttc
     1021 tgtccgctgg cggcgttgat gcacctctac gcgcggttca gccgcttccg catcctcgcc
     1081 gacaagaaaa agtgcatctc gtgcaacgtc tgcacctccg tctgccacca ggggatcgac
     1141 gtgatgaact tcgccaacaa gggattgccg atgggcgacc ccgagtgcgt gcgttgttcc
     1201 gcctgcgtcg gtgcctgtcc gaccggcgta ctgaccttcg gtcaggtcgg ccgcgacggc
     1261 cgcgtgctcg gaaccgaccg gctcgccgcg agcgccgttc gcctgagcga gcggtagggt
     1321 cgctacccgg cttcggccgg cgcgggccgg aggaacgccg cgatctgcgc ggggaagtcg
     1381 gcgagacgga tcggcttggt gatgtagccc gagcaaccgg cgtcgcgcgc ccgctgctcg
     1441 tccgcctccc acgcgtgcgc ggtcagcgcg atcaccggca gatccgccgt cagcgggtcg
     1501 cgcttgatcc gacgcgtcgt ctccaggccg tccatgccgg gcagttggat gtccatcagc
     1561 accagagccg gacggcgctc gagcaggacg cgcagtccct cctctccgct ggccgcctcc
     1621 cacacttcgt agccccgcga ccgcagcacg ttgcgggcga gcttgcgatt gaccgggttg
     1681 tcttcgatga cgaggatcgc cggaggctgg tcatccgacg gcggggtcgc cggacctcga
     1741 ctcgccggcg gcggcggcgc tcgccggtcg gcgccgggcg gtggctcggc cgcggcgacg
     1801 acggcgtcgg ggaatcgcag cgagaaggtc gaccctgcgc cgggcgtcga gcgcacctcg
     1861 accgatccgc cgtgcatccc gaccatcttg cgcacgagcg caagaccgag gcccgtccct
     1921 tcgtagctcc gcgagtacga cccgtcgact tgaaagaact cggtgaagat ccgctcgaga
     1981 tcgccggcct cgatgccgat gccgctgtcg atcacccgac agctcaggtc cgtgccgtcc
     2041 gcagagatct cgacccgcac ccagccgccc gccggtgtga acttgatcgc gttgctcaac
     2101 aggttgagca gcacctggcg gtacattccg gggtcgaggt tgaccgcgat cgatcgtcgc
     2161 ccgtcgtcga cggtgagctc gagaccctcg accgccgcct ggtgtcgcac catcgcgacg
     2221 gtgtccgacg tcagcttggc ggcgtcgaac cgctcgcggt gcacctccat cttgcccgcc
     2281 tcgatcttgc tcaggtcgag gatcccgttg atcagccgca gcaggtgctc gccgctctcg
     2341 ttgatgtcgc ggacgaactc gcgttgctgc tcgccgagcg cgccgaactt gcgatcggac
     2401 agcaacccgg agaagccgat gatcgcgttg agcggcgtgc gcagctcgtg cgagacgttg
     2461 gccaggaact cgctcttcgc ccggttggcg tcggtcagcc gctcgttggc ctgtcgcagc
     2521 cgggtgttga cctgctcgat gcccgacagc gcctgctgca gctcgctcgt ccgttcggcg
     2581 accttgtcct cgagctgctg gttcagcccc tcgactcgat cgatccgttg ctgctgggct
     2641 tcctggcgct ccttgtgcgt gcgatagaac gcggccaaca gccagcacgg cgggacgccg
     2701 agcgcgagac ccgagggacc ggcgacctgc agcacgtgga gcagaccgac cgcgagcgtc
     2761 aatccgccgt aggtcgtgac cgccatccat agtcccgact cgcgccaggt cgcgaggaac
     2821 gggcggcggc cgtcgatcga gatcgcggcg gtcacgagcc cggtgttgag caggaaatag
     2881 acggtcgccg cgaacagcag cggccacacc tgcgcggcgg tcggcgctcc cggcgagccg
     2941 ccgagcgcca ggtagacggc cgacgcgagc gccgtcgaca gcacgacgtt gccgaggttg
     3001 aacgcgatgt gccgcacgtg ccggccgctt tcgcgggcga ccgtcgagcc gaccacgccg
     3061 gccgaggcga cgatgcacgc cgacaccggt ccgaaggcgg cgagtgccgt cagcaggaac
     3121 gggtcggtcg ccgataccga ggtcttcatc cccggcagtc gcacgggcgt cgcgccggcg
     3181 agcgcgatca acgtcgccag caggaacgtc ccgctccagt ccggattgcc gtggcggccg
     3241 aacagcagcg cgaacgagcc gaccgccagc aggtcgacga ggaccacgag acggctcagt
     3301 ctgcgtcggt tcattgccgc cggccggcgg ccagatcgac cggaagctcg tccgcgagcc
     3361 gtgcggcggc gggcaacacg gtgcggaagc aggcacctcc ggccggcgag ctctcgatcc
     3421 agatcgagcc gccctgggcc tcgaccacca gccggcagaa ggtcaaaccc agaccgcgat
     3481 tggtggtcgc cgtcgccggt tcgctcgagc gaacctgagc gtacttgtcg aagacgtgct
     3541 cgcgcagttg ctccggcacc ccgggccctt ggtcgcttac gacgatctcg gcgccttcct
     3601 cgcggtagcc gacctggagc tcgatgaagc cgccggtcgg cgagtggctc atcgcgttcg
     3661 acagcaggtt gtcgatcacc cgacgcgtca gcaaggggtc ggccgtggcc cacaccggcc
     3721 cgtcgacccg catgacgatc tcgatctgcc gctgctcggc cggcgtcgcc gcctcgtcgg
     3781 cggaggctcg cgccagctcg gtcagctcga cgcgctcgat cgagggacgg atgcggcctt
     3841 cctccagcgc ctcgacgtcg agcaggtcga ggatcatgcg cagcatgcgc cgggccccct
     3901 gcttgctgcg gtcgatcatg tggtgcagcg cttcgtcgct gcgcgcgtgg agcagatcga
     3961 gattgcccag caccgcggac agcgggctct tcaggtcgtg caccagggtt tgggccaggg
     4021 tcttcttgag ctgcagctct tcgttgcgcc gcgccagctc gtcgcgcgcg cgctcgagct
     4081 caccgagcaa gtgccgggcc cgcagcaacg cgcgcacctt ggccaagaac tcctcgcgac
     4141 ggacgggctt cgacaggtag tcgtcggccc cggtgtcgag accggtcacc tggtccggca
     4201 tgcgatcgag cgccgtgacc agcatcacct ggcacagccg ggtcgccgga tcacccttga
     4261 gcgcgcgaca gacctcgtga cccgtcatct gcggcatcat cacgtcgagc a
//
LOCUS       DASPGN010000093         7268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1539097, whole genome shotgun sequence.
ACCESSION   DASPGN010000093 DASPGN010000000
VERSION     DASPGN010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539097"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..6384
                     /locus_tag="VD788_02570"
     CDS             28..6384
                     /locus_tag="VD788_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HXV75174.1"
                     /translation="MRPTRIGPYDDVSAPPSVEGLAAAPSPPGCLVGDPVARRIYAQA
                     RDGRGFFRYDAEDDAWQPLADAPFAATGACGAVQVAGTIYTAYAGGARLGVYDVDDDF
                     WTTIPAAVPAAVLAADGDGLLYQVAGRDARRFDPRDGTIETLAQPPFDFAAGASALAG
                     LDRVLLGHGSDGDEGRFASYDPATDEWSSLAPPPGAGAGAAVDPVNRAYLITAPVGGG
                     LYRYDVDRAAWQTIVLPAGVAVDGPVAWLSAPASGFYFGGGTDWTRRSVPPGFILATP
                     SRGVVPPGGEAEISVRFDASDRFGGDHEAELSIRTNDPQLPLARVPARMTVVGLPQIG
                     ALAGPIDFGPVFVGTSRRIDLAIDNLGTAPLSLVVAADRATFTASPATLEVPARETAQ
                     LSLYYAPAAAGAESAWLELTTNDPATPVVRLDLTGSGVDRPIVTITPQTLDSELIEDT
                     TTTHALTIANAGLGPLEFSIAVETAAISFITTGVESGSVPPGESLSVPVFFDGALPGP
                     GTHAARLVVTTNDPFVPQWTIPASLTVRGLPRLALGGEPVQLESTIAFGIYALNTVHE
                     IPIVIPPAGSGTIEIEIEGDYGNDGEFAIATAEGGLLGQIGDASADCAVTSRIFPIDA
                     ARLAGLTHDRLLEIDVANVDSVRPFCPSNRHTVRVTYVGATDRLDFGTVFVGGARPLG
                     LIVENLGSAPLELTASSDDASFAPEPANLQVAPGTAAALRVEFAPLAVGQFDGTLRLL
                     SNDPRAPDPAIVLAGRGAEPPALRLDTDEISVTLLEGGRSSHPLVLANDGAGPLEFIL
                     SISADPPDFVRGDVTSGTIAPGASRTVALSFDAAPLAVGTWPATLEVVSNDLHRPQVV
                     VPLTVDVVEAPNLEVLGQRVTLESSIEFDAVGAVTHHRLPLELAATGGAQIDVVGDGD
                     FGASSEFAIVRAEGVVLGSIGTTGSDCIPAVGRFSIDADRLAALVDDGLVEIDVFNGT
                     TVDAICEKNAHTVRLHYDAIASVIDFGDVFVGTERSRAVRLRNAGQRPLQVESITSDT
                     PSLVPARTALVVAPGAAEVVEIDFRPTTSGPVDAALSIASDDPDSPTIVVPLVGHASD
                     PPVLSVAPASIEVALDEGSSRVETLTVSNHGATPLEFDLVPAAPAGFDPGYTLFATDG
                     GGGNLYTLDSGTGAAVFVGPMGIGAPALAVDPTTGVMYVGQGGGRSWLYAVDPASAVL
                     TAIGDSGLGVSGYAALEFDAEGVLYAAVNVTDGAGTGGDSLAIVDELTGEARVVGSFG
                     GGIGTAGGSPGIGGIAFDPASGILLGASGAQSATIGPPALYSIDLVTGEAIFLRRIVD
                     TAGQPPAGGIGSLQFDGQGRLFAGTGRGTGNLLRIDPATGIYTLVGHLFPSAVGGLAL
                     RADPVDLSFVDVSPTSGTVAPGETASLVVTADARALSPGRYQARLVLRSNDPERPEAA
                     ISLAIDVSGIPDIALPGESVSLDSTRSFSGVGAGTTHRLEIVAASAGGARLQVTANGD
                     FGAVNEVADVHVDGVALGRVGHARSDCFPARKTFVIAAGDFATIAADGEVEVTIDNAP
                     TVGEFCGDSTHAVSLIYDQPRERVDFGTVFPGHPVTRPLTLRNDGRAELHIDAIASDR
                     AEVTVDPTTPLVVPPFGSVELAMTYRPTGAGDLAGSLSIESDDPDEPLLVIALDGRAV
                     GAPRAVISPGTIEAALPPGVGRTTERILQLSNDGDSELRFQVELIETPAEPPPVGQWG
                     DLGKGDGSADGVGSNTVLRSDGPDAFGYRYRDGDDPAGPRFDWIDLTLAGQPLELVGD
                     DRTSAALPLGFSFPFYGASFDAVHVSSNGWLSLSSDKTSYSNPDSLPNAGFSVPENLI
                     APFWDDLDLRQADAVRYLADGDRFVVQYTEVDRFASVAELTFQVVLHRSGRIVFHYLR
                     MDGALDSATIGIQNGDRTIGLLVAYNEPYVRDGLAVEFAPLAPWAEITPAAGTVAAGQ
                     SVELRLALDGGGLVEGDYAARLRLRSNDPANGQIDVPIALRAATVELSRFEIQPRVLN
                     TASRGASVQAVLEFPPGYDPRRVVLASVTLDGVVDADPGNWRVEDGDADGREELVVSF
                     DRRAVLDWVGTAGGRDVTVSGEIAGQAWFEGTTTVSTIDRGRER"
     gene            6397..>7268
                     /locus_tag="VD788_02575"
     CDS             6397..>7268
                     /locus_tag="VD788_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75175.1"
                     /translation="MRSSVIVCAALLAVGGVTADEPAPGSGAEVVRELSEQAARLAPF
                     VESAVARRFLAAAPALPPIEDARRVHFNESTREAVTPAVAAGMSADALRGFEPRELDG
                     RFYYFTRYGTPLAFVRALDLLGRAGLDGLAGARLVDFGFGSIGHLRMMASSGAEVVGI
                     EVDPLLEALYADPTDVGRIPPAGGGGDESGRLELLFGRFPADGATVERLGTGYDVFVS
                     KNTLKRGYIHPDQPVDPARLVRLDVDDERFVRAVHALLEPGGLFLIYNLYPPQADDPA
                     SYRPWADGRSPFSL"
BASE COUNT          955 a         2625 c         2583 g         1105 t
ORIGIN      
        1 ggctcgccgc tcgcgttcga gctgtgggtg cgaccgacgc gtatcgggcc gtacgacgac
       61 gtctccgctc ccccgtcggt cgagggcctg gccgccgcgc cgtcgccgcc gggctgcctg
      121 gtcggcgacc cggtcgcccg ccggatctat gcccaggccc gggacggtcg cggcttcttc
      181 cgttacgacg cggaggacga cgcgtggcaa ccgctggcgg atgcgccgtt cgcggcgact
      241 ggcgcctgtg gggcggtgca ggtggcgggc acgatctaca ccgcctacgc cggtggcgca
      301 cgcctcgggg tctacgacgt ggacgacgac ttctggacga cgatcccggc cgccgttccc
      361 gcggcggtgc tggccgccga cggcgacggc ctgctgtacc aggtcgccgg acgcgatgcg
      421 cggagattcg atcctcgaga cgggacgatc gaaacgctgg cccaaccgcc gttcgacttc
      481 gccgcgggcg cgagcgcgct cgccggcctc gaccgggtgc tgctgggtca cggcagcgac
      541 ggcgacgagg gacggttcgc ctcgtacgac ccggcgaccg acgagtggtc gtcgctcgcg
      601 ccgcctcccg gggccggcgc gggcgccgcg gtcgacccgg tcaaccgagc ctatctgatc
      661 accgcccccg tgggaggtgg gttgtatcgc tacgacgtcg accgggccgc gtggcagacg
      721 attgtgctgc cggcgggcgt ggccgtcgac ggccccgtcg cctggttgtc cgcgccggct
      781 tccggcttct acttcggcgg cggaacggac tggacgcgcc ggagcgtgcc gcccggcttc
      841 atcctcgcga cgccctcccg cggcgtcgtc ccgccgggag gcgaggccga gatctcggtc
      901 cgcttcgacg cgagcgatcg cttcggcggc gaccacgaag cggagttgtc gatccgaacc
      961 aacgaccccc aactcccgct cgcgcgggta ccggcgcgaa tgaccgtcgt cggtctgccg
     1021 cagatcggcg cgctcgccgg gccgatcgac ttcggcccgg tcttcgtcgg cacctcgcgg
     1081 cggatcgatc tcgcgatcga caatctcggc accgcgccgt tgtcgttggt cgtcgcggcc
     1141 gaccgcgcga cgttcaccgc ctcgccggcg acgctcgagg ttccggcgcg cgagaccgcg
     1201 caactgagcc tgtactacgc gcccgcagcg gcgggcgccg agagcgcgtg gctcgagctg
     1261 acgaccaacg atccggccac gccggtcgtg cggctcgatc tcaccggctc cggcgtcgat
     1321 cggccgatcg tgacgatcac gccgcagacg ctcgacagcg agctgatcga ggacaccacg
     1381 acgactcacg cgctgacgat cgccaacgcc gggctcggcc cgctcgagtt ctcgatcgcc
     1441 gtcgagacgg ccgcgatctc gttcatcacg accggcgtcg agtcggggag cgttccgccc
     1501 ggcgagtcgc tttcggtccc ggtcttcttc gacggcgcgc ttcccgggcc cgggacccac
     1561 gcggcgcggc tcgtcgtcac caccaacgac ccgttcgttc cgcaatggac gataccggcg
     1621 tcgttgaccg ttcgcggcct cccgcggctc gcgctcggag gcgagccggt ccagctggaa
     1681 tcgacgatcg cgttcgggat ctacgcgctc aacaccgtcc acgagatacc gatcgtcatt
     1741 ccaccggcgg gcagcgggac gatcgagatc gagatcgagg gtgactacgg caacgacggc
     1801 gagttcgcga tcgccaccgc cgagggtggt ctgctgggcc agatcggcga cgcatcggcc
     1861 gactgcgccg tgacgtcgcg gatctttccg atcgacgcgg cgcgcctcgc cggactgacg
     1921 cacgaccggc tgctcgagat cgacgtcgcg aacgtcgact cggtgcggcc gttctgtccg
     1981 tccaaccggc ataccgtccg ggtcacctac gtcggcgcga ccgatcgact cgacttcggc
     2041 accgtcttcg tcggcggcgc gcgcccgctc ggcttgatcg tcgagaacct cgggtctgcg
     2101 ccgctcgagc tgaccgcgtc gagcgacgac gcgtcgttcg cgccggagcc ggcgaacctg
     2161 caggtcgcgc ccggcaccgc ggccgcgttg cgcgtcgagt tcgccccgct cgccgtcggt
     2221 cagttcgacg gcacgctgcg tctgctgagc aacgatcccc gagctcccga tccggcgatc
     2281 gtgctcgccg gtcgcggcgc cgaaccaccc gcgctgcgtc tcgacaccga cgagatctcg
     2341 gtaacgctgc tcgagggcgg tcgttcgtca cacccgttgg tcctggccaa cgacggtgcg
     2401 ggcccgctcg agttcatcct gtcgatctcg gccgaccctc ccgacttcgt ccgcggcgac
     2461 gtgaccagcg ggacgatcgc gcccggggcg agccgcacgg tcgcgctgtc gttcgacgcc
     2521 gcaccgctgg ccgtcggtac gtggccggcg acgctcgagg tcgtctcgaa cgacctgcac
     2581 cggccgcagg tcgtcgtgcc gctgaccgtg gacgtcgtcg aggcgccgaa cctcgaggtg
     2641 ctgggccagc gcgtcacgct cgagtcgtcg atcgagttcg acgcggtcgg cgccgtgacg
     2701 caccaccgcc tgccgctcga gctcgcggcg accggcggcg cgcagatcga cgtggtcggc
     2761 gacggcgact tcggtgcctc gagcgagttc gcgatcgtga gggcggaggg agtcgtcctc
     2821 ggatcgatcg gcacgacggg gagcgactgc atcccggcgg tcggtcgttt ttcgatcgac
     2881 gccgaccggc tcgccgcact ggtcgacgac ggcctggtcg agatcgacgt gttcaacggc
     2941 acgacggtcg acgcgatctg cgagaagaac gcgcacacgg ttcgcctgca ttacgatgcg
     3001 atcgcgagcg tgatcgactt cggcgacgtg ttcgtcggta ccgagcgttc gcgggccgtc
     3061 cggttgagga acgccggcca gcgaccgctg caggtcgagt cgatcaccag cgacacgccg
     3121 agcctggttc ccgcgcggac cgcgctcgtg gtcgcgcccg gcgcggcgga ggtcgtcgag
     3181 atcgacttcc gtccgacgac gagcggcccg gtcgacgccg cgctgtcgat cgcgagcgac
     3241 gaccccgaca gcccgacgat cgtcgtgccg ctcgtcgggc acgcgtcgga tccgccggtg
     3301 ttgtcggtcg cgccggcgtc gatcgaggtc gcgctcgacg agggctcctc gcgcgtcgag
     3361 acgctgaccg tgagtaacca cggcgcgacc ccgctcgagt tcgacctggt gccggcggcg
     3421 cccgctggct tcgacccggg ctacaccttg ttcgccaccg acggtggcgg cgggaatctc
     3481 tacacgctcg acagcggaac cggcgccgcc gtgttcgtcg gcccgatggg catcggcgca
     3541 ccggcgctgg ccgtcgaccc gacgacggga gtgatgtacg tcggtcaggg cggtggccgc
     3601 tcgtggctct acgccgtcga cccggcgagc gccgtgctga ccgccatcgg cgacagcggc
     3661 ctcggcgtct cgggctacgc ggcgctcgag ttcgacgcgg agggcgtgct gtacgccgcc
     3721 gtcaacgtga cggacggcgc cggcaccggc ggcgactcgc tcgcgatcgt cgacgagctg
     3781 acgggtgagg cgcgggtcgt cggatcgttc ggcgggggca tcggcaccgc cggcggaagc
     3841 cccggaatcg gcgggatcgc cttcgacccc gccagcggta tcctgctcgg agcgtcggga
     3901 gcgcagtccg cgacgatcgg accgcccgcg ctctactcga tcgacctcgt caccggcgag
     3961 gcgatcttcc tcagacggat cgtcgacacc gcgggccagc cccccgcggg cgggatcggc
     4021 agcctgcagt tcgacggcca aggccgactg ttcgccggca ccggtcgcgg aacgggcaac
     4081 ctgctgcgca tcgatccggc aaccggcatc tacacgctcg tcggccacct gttccccagc
     4141 gccgtcggcg ggctggcgct gcgcgccgac ccggtcgacc tgtcgttcgt cgacgtctca
     4201 ccgaccagcg gcaccgtcgc acccggcgag accgcctcgc tcgtcgtcac ggccgacgcg
     4261 cgcgcgctgt cgcccggccg ctaccaagcc cggctggtgc tgcgcagcaa cgaccccgag
     4321 cgaccggagg ccgcgatctc gctcgcgatc gacgtcagcg gcattcccga catcgccctt
     4381 cccggcgaga gcgtctcgct cgattcgacg cgatcgttct ccggggtcgg cgcgggtacg
     4441 acccaccgtc tcgagatcgt ggcggcgtcc gccggcggcg cccgcctgca ggtgacggcg
     4501 aacggcgact tcggcgcggt caacgaggtg gccgacgtgc acgtcgacgg cgtcgcgttg
     4561 ggtcgagtcg gtcacgcgcg ctccgactgc ttcccggcgc gcaagacctt cgtcatcgcg
     4621 gcgggtgact tcgccacgat cgctgccgac ggggaggtcg aggtcacgat cgacaacgcg
     4681 ccgactgtcg gcgagttctg cggcgacagc acgcacgcgg tctcgctgat ctacgaccag
     4741 ccgcgcgaac gggtcgattt cggtacggtc ttccccggac acccggtgac ccgaccgctg
     4801 accttgcgca acgacggccg ggccgagctt cacatcgacg cgatcgccag cgaccgggcg
     4861 gaggtgaccg tcgatcccac gaccccgctg gtcgtgccgc cgttcggttc ggtcgagctg
     4921 gcgatgacct accgcccgac cggggccggc gacctcgccg gatcgttgag catcgaatcc
     4981 gacgaccccg acgagccgct gctcgtgatc gctctcgacg gccgtgccgt cggcgcgccg
     5041 cgcgcggtga tctctcccgg cacgatcgag gcggcgctgc cacccggcgt cgggcggacg
     5101 accgagcgga ttttgcagtt gtcgaacgac ggggacagcg agctgcgttt ccaggtcgag
     5161 ctgatcgaga cgccggccga gccgccaccg gtgggacagt ggggcgatct gggcaagggc
     5221 gacgggtcgg ccgatggggt cggatcgaac accgtcctgc gcagcgacgg gcccgacgcg
     5281 ttcggctacc gctaccgcga cggcgacgat ccggccgggc cgcggttcga ctggatcgac
     5341 ctcacgctcg ccggtcagcc gctcgagctg gtcggcgacg accgcaccag cgccgcgttg
     5401 ccgctcggat tctccttccc gttctacggc gcctcgttcg acgccgtcca cgtctcgtcc
     5461 aacggctggc tcagcttgtc gagcgacaag accagctaca gcaacccgga ttcgttgccc
     5521 aacgcgggct tctcggtgcc ggagaacctg atcgccccgt tctgggacga cctcgaccta
     5581 cgccaggcgg acgcggtgcg ctatctggcc gacggcgatc gcttcgtcgt gcagtacacc
     5641 gaggtcgacc gcttcgcctc ggtcgccgag ctgacgttcc aggtcgtgct gcaccgatcg
     5701 gggagaatcg tcttccacta cctgcggatg gacggcgcgc tcgacagcgc gacgatcggc
     5761 atccagaacg gcgaccgcac gatcgggctg ttggtcgcct acaacgagcc gtacgtgcgc
     5821 gacggcctcg cggtcgagtt cgcccccctc gcgccctggg ccgagatcac gccggcggcc
     5881 gggacggtcg ctgccggcca gtccgtcgag ctgcggctgg cgctcgacgg cggcgggctc
     5941 gtcgagggcg attatgcggc gcggctgcgc ctgcgttcga acgatcccgc caacggacag
     6001 atcgacgtac cgatcgcgct gcgcgccgcg accgtcgagc tgagccgctt cgagatccag
     6061 ccgcgcgtgc tcaacaccgc ctcgcgcggc gcgtcggtcc aagcggtcct cgagtttccg
     6121 ccgggctacg acccgcgccg cgtggtgctc gcgagcgtga cgctcgacgg cgtggtggac
     6181 gccgatcccg gcaattggcg cgtggaagac ggcgacgcgg acggtcgcga ggagctggtc
     6241 gtgagcttcg accgccgtgc cgtgctcgac tgggtcggca ccgccggcgg tcgcgacgtc
     6301 accgtctcgg gcgagatcgc cggacaggcc tggttcgagg ggacgaccac ggtttcgacg
     6361 atcgaccgtg ggcgcgaacg atagggggac gaagcgatgc gatcgagcgt gatcgtctgt
     6421 gcggcgctgt tggcggttgg cggcgtgacg gccgacgaac cggccccagg cagcggcgcc
     6481 gaggtcgtgc gcgagctgag cgaacaggcc gcgcggctgg ccccgttcgt cgagtcggcg
     6541 gtcgcgcggc gctttctcgc cgcggcgccg gcgctgcccc cgatcgagga cgctcgccgg
     6601 gtccacttca acgagtcgac gcgcgaagcc gtcacgccgg ccgtcgcggc cgggatgagc
     6661 gccgacgcgt tgcgcggctt cgagccccgg gagctcgacg gtcggttcta ctacttcacg
     6721 cgctacggca ccccgctcgc cttcgtccgc gccctcgacc tgttgggacg cgccgggctg
     6781 gacgggctcg ccggtgctcg gctggtcgac ttcggcttcg gctcgatcgg gcacctgcgg
     6841 atgatggcgt cgtcgggggc cgaagtagtc ggcatcgagg tcgacccgtt gctcgaagcg
     6901 ctctacgccg acccgacgga cgtcggacgc attccaccgg ccggcggcgg gggcgacgag
     6961 tcggggcggc tcgagctgtt gttcggccgg ttccccgcgg acggcgcgac cgtcgagcgg
     7021 ctcggtacgg gttacgacgt tttcgtctcg aagaacacgt tgaaacgggg ctacatccat
     7081 cccgatcagc cggtcgatcc ggcgcgactg gttcggctgg acgtcgacga cgagcggttc
     7141 gtccgcgcgg tccacgcgtt gctcgagccg ggtggtctgt ttctgatcta caacctctac
     7201 ccgccgcaag cggacgaccc ggcgagctac cgcccgtggg ccgatggccg ctcgccgttc
     7261 tcgctcga
//
LOCUS       DASPGN010000094        11397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_367624, whole genome shotgun sequence.
ACCESSION   DASPGN010000094 DASPGN010000000
VERSION     DASPGN010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11397
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367624"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VD788_02580"
     CDS             <1..507
                     /locus_tag="VD788_02580"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXV75176.1"
                     /translation="TVVAAGPGSPGLRELLAFYDVGSDHGRDALDAEERAMQIFAAVL
                     PTKRRSSEPKRPATARLHRRVVDRACEYLIEYSSGSISLREVARHAGASPAHLSRLFK
                     RHKGLSMGDYLLRLRLSAVVDALERRPHGLDRIARDAGFCSHSHLTTVFRRELGRTPN
                     QVRRLFGG"
     gene            605..3916
                     /locus_tag="VD788_02585"
     CDS             605..3916
                     /locus_tag="VD788_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXV75177.1"
                     /translation="MTRLSCRIAAGLTACAATLASAGESLFMRAPAISPDGQTIAFNY
                     RGDLWTVPAAGGKATPLTLHDAYDSHPVWSRDGRRIAFASDRYGNFDVWVIPAAGGPA
                     ERLTFHSAADIPTAFNPSDDRVIFHSSRIDAVDCVQFPASAQPELYDVSLAGGMPRQL
                     LTTPALDAVLDRDGARLAYTDRKGYENEWRKHDSSSFARDVWVVDLATGEHARLTEFG
                     YDDRQPVWSPDQRSLFFLSERSGSFNVWRLDLDAPAEPEQITRHDTHPVRDLSIAADG
                     TLAYSYDGAIYTRAPGARRSTRISVDAESDLRRNATGMRDVSRNISEFDVSPDGKEIV
                     FVARGEIFVTSANHDATRQISRTPEQERSVSFHPNGRSLLYASERGGSWNLYRMDLTD
                     EDELNFFNATSMKETPILEIAVETFQPRFSPDGKRVAYLKERTQLEVIDLESGTRRTI
                     LAGDRNYSYVDGDQNYDWSPSGEHFLVDFLSPTRWSREVGLVPSDGEGELVNLTRSGY
                     GDVAGRWAVEGNAMFWYTDRWGGQLQRGGPVEYDVKLAFFNDESWDRYRLSEVELEQL
                     TELEKRAKKDGKKKDANKDEAKGDGDEQGEGDEVEDERKADEDEGDELELPEPVELEL
                     DRLEDRTTRLTIHSSRLASATLTPDGERLLYLAQFEKGFDLWSYEHRKQEIKLLAKLD
                     ADSADNLLLDKEGKTAFLLADGSIRTVEIASGKVKPVSLSARIELDAAAERDYLYEHA
                     WRQTLKKFHDVDMHGVDWKMYKEAYARFLPYVDHNRDFAYLLSELLGELNASHTGAYY
                     NASRDGNGGGDETASLGLFPDPDWDGSGIRVLEILPKSPLTQAGSRIGVGTVIMAIDG
                     QTIDAGQNWYPLLNHKAGQNVRLTLRDEQDETWEEVVRPIGSDADLLYERWVRSRRAE
                     VDRLSGGRIGYAHIRGMNEGAFREIFDEIFGESVDKQAIVLDTRFNGGGNLDEALPVF
                     LSGQTYMRAKPRGQAIGSIPERRWTKPSILIQNEGNYSDAHCVPNAYRSLGLGKTVGM
                     QVPGTCTSVWWEQLQDPSITFGIPQVTWIDNAGDPLENKHFDPDFEIDNDPKLEARGR
                     DQQLEKAVEFLLRQL"
     gene            3891..4157
                     /locus_tag="VD788_02590"
     CDS             3891..4157
                     /locus_tag="VD788_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75178.1"
                     /translation="MSSCCDNCSRLLSLAVAAIGSHSIAAGDHGPARELAPYVRIDAP
                     RVALRQVALLDGTGAGRRERQTVLIDRGRLQAVGPSLASISAAA"
     gene            4283..5167
                     /locus_tag="VD788_02595"
     CDS             4283..5167
                     /locus_tag="VD788_02595"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV75179.1"
                     /translation="MEPRLARVVIRQAHRRGARVTGHLCSIGFVEAAMLGIDNLEHGL
                     FGNSEYDPRKRAGQCPPADHRAFEWLDLDGRQVRRTFDTLIERGVALTSTLPVLETLV
                     AGRPDPIDPRMHELLHPAVLERYLETYAAIRDVPGAGISTAAFRRIMDYDVAFARAGG
                     LLAAGVDSSGTGGALAGLGDQRNLELLVEAGFTVPEAVRVMTLNGARLLGVDDELGSV
                     EPGKIADLVVVAGDLVEDPAAIRQVVHVFKDGIAYDPSRLLDSVKGRRALTRGATRAR
                     TFARPPVRPTARTLCRAA"
     gene            complement(5097..5564)
                     /locus_tag="VD788_02600"
     CDS             complement(5097..5564)
                     /locus_tag="VD788_02600"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HXV75180.1"
                     /translation="MPMRKLERYLDDHGVKYLRIRHSPAFTAQEIAASAHIPGKHLAK
                     TVVVKLDGRLAMIVLPASNRIDFDLLRDEVGAERIELAEEREFRDLFPDCELGAMPPF
                     GNLWNVPVFVDDELTRDDEIAFNAGSHTELLKLPYREFARLVGPAVVRTCERV"
     gene            complement(5734..6150)
                     /locus_tag="VD788_02605"
     CDS             complement(5734..6150)
                     /locus_tag="VD788_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXV75181.1"
                     /translation="MSIMKEATKKEMTQREKQPVSGAESTRPGRVFTPVVDIYETDGA
                     ITLLADMPGVTSEGLAIDLRDNVLTIDGSVDDVGGPNEVLLLREYDTGAYRREFRLTN
                     LIDQARIEATLKDGVLKLTLPKAEAARPRRIEVKTR"
     gene            complement(6173..6556)
                     /locus_tag="VD788_02610"
     CDS             complement(6173..6556)
                     /locus_tag="VD788_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXV75182.1"
                     /translation="MDELFDALGEITRYRSAGVFPAINVSEDPESIYVRAELPGCTPG
                     NLNVTVENDTLTISGERHRSQEEQETSFHRREREWGRFRRSFSLNTRVDSAKVDARYT
                     DGILTVVLPKAVEARPKQIPITTGA"
     gene            complement(6782..7633)
                     /locus_tag="VD788_02615"
     CDS             complement(6782..7633)
                     /locus_tag="VD788_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HXV75183.1"
                     /translation="MDDFGRTLRSEREGRGLTIAGLTDKLGVERERLLALERNDLASL
                     PDDATMMPLLRTYADCLGVEAELMIEHYLRERQRCLDGLATRIDAVADVKRTAAAPPV
                     GRSSPFPRLLAASIVVTAVASFGLWWVFFSAGPSAPPRIERAPTAAAPAPAAAEEPTP
                     GRALVPAAASPVAAGSPTLTVAEAAVGTGVRRLEPIGEGERFERGSQVWFWTRVEGGR
                     RGDRVDHVWIEDGVEAARISLELGGRSWRTYSAKTLRPGSAAEWAVEARDASGNVLAR
                     REFIVVR"
     gene            7780..9273
                     /locus_tag="VD788_02620"
     CDS             7780..9273
                     /locus_tag="VD788_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HXV75184.1"
                     /translation="MAAGTRPDRSINVGVRRLEPTGLWNHFADLNAVPRASKKEQRVI
                     RFMRAFGERLALETVVDEVGNVVIRKPASPGMEDRLPIVMQSHLDMVHQKNADTDFDF
                     DTEGIEMVVEGDWVRAAGTTLGADNGIGVATIMTVLAEDAIAHPRLEALFTIDEETGM
                     TGAKGLKGGLLTGRILLNLDTEADDELTIGCAGGVDVTATGRYDPESTPAGQVGLELV
                     VRGLQGGHSGMEIHVGFANANKLMNRLLAGVVRRFGARLSSIDGGGLRNAIPRESFAI
                     VTVSRERRDELAAWVAAEAETIRVEYRTTDPAADVRCTPIDAPRQVVPTELTRLLLGA
                     IDGVHSGIHRMSPEVGGLVQTSNNLARVRVADGEFTLGCLTRSSVDSERDALARSIAA
                     VLELTGADLSTSGSYPGWQPAPESAIVKLMSSIYKEMFDAPAEVSACHAGLECGIIGT
                     NYPGLEMISFGPTILGAHSPDERVKISSVQKYWRFLLETLKRIPAGD"
     gene            complement(9278..9991)
                     /locus_tag="VD788_02625"
     CDS             complement(9278..9991)
                     /locus_tag="VD788_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75185.1"
                     /translation="MSGDGAGFSLVLAAAAVAAIHTVLGPDHYLPFIMLGRSRGWGRV
                     KTAWITTACGSAHVVASWSLAGGGLLLGFAVGRIERMEQARGDLAAWLLIGFGLAYAL
                     WGMRRGLRRASGLEPHTHDGRLHLHLHGDRPHRHFRFPAQRGDAGFWSLFVIFVLGPC
                     EPLIPLFVLPASRGRWDWACVTALVFAVVTVATMVGLTTLGLHGFERLRWRWLERWSQ
                     ALAGGVIAASGLAIVAFGL"
     gene            complement(9995..11002)
                     /locus_tag="VD788_02630"
     CDS             complement(9995..11002)
                     /locus_tag="VD788_02630"
                     /inference="COORDINATES: protein motif:HMM:NF015746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HXV75186.1"
                     /translation="MRDATKPGGAGPKAGWIDRALGRVERAGNRLPDPAMLFVLALVG
                     VWIVSWLLAGHPFTVPARDGDRMLEVQNQATGSALATALSTMVSSFTGFPPLGVVLWA
                     AAWPADSPLHAADGSITSFGAPIMQSIVPLIFLLFLFPAIVYGYIGGTVRSHRDLIVA
                     MTKSMQTMGYYLVMIFFCAQFTAAFGRSNLGALLAVEGADLLRALGLSGFVTIVGIIL
                     LTSVVNLLVGSASGKWALLAPIFVPILMQLGLSPELTQAAYRVGDSTTNIITPLMPYF
                     PLVVAFAQRWVRGSGIGTITSLMLPYTVSLLVSWTIFLVGDRSAAGSTTGLHLPVTSS
                     R"
     gene            complement(11069..>11397)
                     /locus_tag="VD788_02635"
     CDS             complement(11069..>11397)
                     /locus_tag="VD788_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75187.1"
                     /translation="SAVAPTTPAAPRFTDPARTWESLLAALGRGDRVAAEACFVPSAL
                     GALDDVPDASPIGALRALVSSFSRIENVGRVGPYWSIYGVRERRRPKWIFFEETADGE
                     WKIAGL"
BASE COUNT         1955 a         3869 c         3899 g         1674 t
ORIGIN      
        1 acggtcgtcg ccgccgggcc ggggagcccg gggctgcgcg agctgctcgc gttctacgac
       61 gtcgggtcgg accacggccg cgacgcgctc gatgccgagg agcgcgcgat gcagatcttc
      121 gccgcggtgc taccgaccaa acgccgctcg tcggagccca aacggcccgc gacggcacgc
      181 ctgcaccgcc gagttgtcga ccgcgcctgc gagtacttga tcgagtacag ctccgggtcg
      241 atcagcctgc gcgaggtcgc acggcacgcg ggcgcctcgc cggcccacct ctcgcgcctg
      301 ttcaagcggc acaagggcct ctcgatgggc gactatctgc tgcggttgcg gctgtcggcc
      361 gtcgtcgacg cgctcgagcg ccggccgcac gggctcgacc gcatcgcccg cgacgccggt
      421 ttctgcagcc attcccatct gacgaccgtc ttcagacgcg agctcggtcg gacgccgaac
      481 caggtccgcc gcttgttcgg cggctgaggg gaccggccaa acgagcagga tcgttgaagc
      541 cgccgccacc ccgcgccccg tacgatccgt cgatgccgtc gaacggccaa ggagaggctc
      601 gaagatgacc agactatcgt gtcgtatcgc cgcaggcctg acggcctgcg cggcgacgct
      661 ggcctcggcg ggtgaatcgc tgttcatgcg tgccccggcg atctcacccg acggacagac
      721 gatcgcgttc aattaccgcg gcgacctctg gacggtgccg gccgcgggcg gcaaagccac
      781 gccgctgacg cttcacgacg cgtacgacag ccatccggtc tggtcgcgcg acggtcgccg
      841 gatcgccttc gcctccgacc gttacggcaa cttcgatgtc tgggtgatcc cggccgccgg
      901 cggaccggcc gaacggctga ccttccattc cgccgcggac ataccgacgg cgttcaaccc
      961 gagcgacgac cgggtgatct tccactcctc gcggatcgac gcggtcgatt gcgtgcagtt
     1021 tcccgcctcg gcccaaccgg aactgtacga tgtctcgctg gccggcggga tgccgcgtca
     1081 gctgctgacg acccctgccc tcgacgcggt cctcgaccgc gacggagcgc ggctcgcgta
     1141 caccgatcgc aaggggtacg agaacgaatg gcgcaagcac gacagctcgt cgttcgcgcg
     1201 cgacgtatgg gtggtcgacc tcgcgacggg cgagcacgcg cggttgacgg agttcggcta
     1261 cgacgaccgc cagccggtgt ggagcccgga ccagcgctcg ctctttttct tgtcggagcg
     1321 atcggggagc ttcaacgtct ggcgtctcga tctggacgcg ccggccgagc ccgagcagat
     1381 cacacgccac gatacgcacc ccgtacgcga tctgagcatc gccgcggacg gcacgctggc
     1441 ctacagctac gacggcgcga tctacactcg cgcgcccggt gcgcggcgca gcacccggat
     1501 cagcgtcgat gccgagtccg acctgcgccg caacgcgacc ggtatgcggg acgtctcgag
     1561 gaacatcagc gagttcgatg tctcacccga cgggaaagag atcgtgttcg tcgctcgcgg
     1621 tgagatcttc gtcacctcgg ccaaccacga cgccacgcgg cagatcagcc gcacacccga
     1681 gcaagagcgt tcggtcagct tccaccccaa cgggcgcagc ttgctctacg cgtcggagcg
     1741 cggcggaagc tggaacctgt accggatgga cctgaccgac gaggacgagc tgaacttctt
     1801 caatgcgacg tcgatgaagg agacgccgat cctcgagata gccgtcgaga cgttccagcc
     1861 ccgtttctcg cccgacggca aacgggtggc ctacctgaag gagcgcacgc agctcgaggt
     1921 gatcgatctg gaaagcggca cgcgccggac gatcctcgcc ggcgaccgaa actactccta
     1981 cgtcgacggc gaccagaact acgactggtc gccgagcggc gagcactttc tcgtcgactt
     2041 cctcagcccg acccgctggt cgagagaggt cgggctcgtg ccttcggacg gcgagggcga
     2101 gctcgtcaac ctgacgcgca gcggctacgg cgacgtcgcc ggtcggtggg cggtcgaggg
     2161 caacgcgatg ttctggtaca ccgaccgctg gggcggacag ctgcagcgcg gcgggccggt
     2221 cgaatacgac gtcaagctgg cattcttcaa cgacgaatcg tgggaccgat accgcttgtc
     2281 ggaagtcgaa ctggagcagc tgaccgagct ggagaagcgc gcgaagaagg acggcaagaa
     2341 gaaagacgcg aacaaggacg aagccaaggg cgacggggac gagcagggcg agggtgacga
     2401 ggtcgaagac gagagaaagg cggacgagga cgagggggac gagctcgagc tgccggagcc
     2461 ggtcgagctc gagctggacc ggctcgagga ccggacgacg cggctgacga tccattcctc
     2521 ccggttggcc agcgcgacgc tgacgccgga cggtgagcgg ttgctgtacc tggcgcagtt
     2581 cgagaaaggc ttcgacctgt ggagctacga gcatcgcaag caggagatca agctgttggc
     2641 caagctcgac gcggactcgg ccgacaacct gttgctggac aaggagggca agaccgcttt
     2701 cctgctggcc gacggctcga tccgaacggt cgagatcgcc tcgggcaagg tcaagccggt
     2761 gtcgctgtcg gccaggatcg agctcgatgc cgcggccgag cgcgactacc tttacgagca
     2821 cgcctggcgg cagacgctca agaagttcca cgacgtcgac atgcacggcg tcgattggaa
     2881 gatgtacaag gaagcgtacg cgcggttcct gccctacgtc gatcacaacc gcgacttcgc
     2941 ctacctgttg tcggagctgc tcggcgagtt gaacgcctcg cacaccgggg cctactacaa
     3001 cgcgagccgc gacggcaacg gtgggggaga cgagaccgcc agtctggggt tgttccccga
     3061 ccccgactgg gacggatcgg gaatccgcgt gctcgagatc ctgcccaaga gcccgctgac
     3121 gcaggccgga agccgcatcg gcgtcgggac cgtgatcatg gcgatcgacg gccaaacgat
     3181 cgacgccggc cagaactggt acccgctgct caaccacaag gccggccaga acgtccggct
     3241 gacgttgcgt gacgagcagg acgagacgtg ggaagaggtg gtccgaccga tcggctccga
     3301 cgccgacctg ctgtacgagc gctgggttcg ctcgcgccga gccgaggtag accgattgtc
     3361 gggcggtcgc atcggctacg cgcacatccg cgggatgaac gagggtgcgt ttcgcgagat
     3421 cttcgatgag atcttcggcg agtcggtcga caagcaggcg atcgtcctcg acacgcgttt
     3481 caacggcggg gggaacctcg acgaggcgct gccggtcttt ctctcggggc agacctacat
     3541 gcgggccaag ccacgcggtc aggcgatcgg ctcgatcccc gagcgccgct ggaccaagcc
     3601 gtcgatcctg atccagaacg aggggaacta ctccgacgcg cactgcgtgc ccaacgcgta
     3661 ccgctcgctg gggctgggca agaccgtcgg catgcaggtg ccgggcacct gcacctcggt
     3721 ttggtgggaa cagctgcagg atccgagtat caccttcggc atccctcaag tcacctggat
     3781 cgacaacgcg ggcgacccgc tggagaacaa gcacttcgat ccggacttcg agatcgacaa
     3841 cgacccgaag ctcgaagcac gcggtcgcga tcagcagctc gagaaggcgg ttgagttcct
     3901 gctgcgacaa ctgtagccgc ctgctgtcgc tggcagtcgc tgcgatcgga tcgcactcga
     3961 tcgcggccgg cgaccacggg ccggctcgcg agctcgcgcc ctacgtcagg atcgacgcac
     4021 cgcgggtcgc gctgcggcag gtcgcgttgc tcgacggaac cggggccggg cggcgcgaac
     4081 ggcagaccgt gttgatcgat cgcggccggc tacaggcggt cggcccgagc ctcgcctcta
     4141 tctcggctgc ggcgtgacga cgatccgcgt caccggctcc tacgccccct acgaagacct
     4201 gcacgtcaag cgcgagatcg acgccggtcg ctcgcccggg ccgcgcgtcc actgaccgac
     4261 ctggttcaag ctctacacga cgatcgagcc gcgcctggcg cgcgtcgtga tccggcaggc
     4321 gcatcggcgc ggtgcgcggg tgacgggaca cctgtgcagc atcggcttcg tcgaggccgc
     4381 gatgctgggc atcgacaacc tggagcacgg gttgttcggc aacagcgagt acgatccgcg
     4441 caagcgagcc gggcagtgcc cgcccgccga ccacagggcg ttcgagtggc tcgacctcga
     4501 cgggcggcag gtgcggcgga ccttcgacac gctgatcgag cgaggggtgg cgctgacctc
     4561 gacgcttccg gtgctcgaga cgctcgtcgc cggacgaccc gacccgatcg acccgcggat
     4621 gcacgagctg ttgcaccccg cggtgctcga gcgctacctc gagacctacg cggcgatccg
     4681 cgatgttccg ggcgccggga tctcgaccgc agcgttccgt cggatcatgg actacgacgt
     4741 ggcgttcgcg cgcgcgggtg ggttgttggc tgccggcgtc gacagctccg gaaccggcgg
     4801 cgcgctggcg gggctcggcg atcaacgcaa tctggaactg ctggtcgagg ccggtttcac
     4861 cgtgcccgaa gcggtgcgcg tgatgacgtt gaacggcgcc cgcctgctcg gcgtggacga
     4921 cgagttgggc agcgtcgagc cgggtaagat cgccgatctg gtcgtcgtcg ccggcgacct
     4981 ggtggaagat ccggccgcga tccgtcaggt ggtccacgtg ttcaaggacg gcatcgcgta
     5041 cgacccgtcg cggctgctcg attcggtcaa gggtcggcgt gcgctgacgc gcggcgctac
     5101 acgcgctcgc acgttcgcac gaccgccggt ccgaccaacc gcgcgaactc tctgtagggc
     5161 agcttgagca gttccgtatg cgagccggcg ttgaacgcga tctcgtcgtc ccgggtcaac
     5221 tcgtcgtcga cgaagaccgg gacgttccac aggttgccga acggcggcat cgctcccagc
     5281 tcgcagtcgg gaaacaggtc acggaattcc cgctcctccg ccagctcgat ccgctccgca
     5341 ccgacctcgt cgcgcagcag atcgaagtcg atgcggttcg atgcaggcag gacgatcatc
     5401 gccaacctgc cgtcgagctt gaccaccacg gttttcgcca ggtgcttgcc cgggatgtgg
     5461 gccgacgccg cgatctcctg ggcggtgaac gcgggcgagt gacggatcct gaggtacttg
     5521 accccgtgat cgtcgagata tcgttcgagc ttgcgcatcg gcatggcgca cctccaactc
     5581 ggcgggagtc gccgaaagac ctgtcattag cttactcccc tcgcggtcgg aggaccggcg
     5641 cccaatcgcg acccgttcgg tcgcttgcgc cgggaggagg agaatcggtg ccgcggcccg
     5701 gtcgcccgac gcgggccgcg gcggagcgtt ggttcagcgg gtcttgacct cgatgcgtcg
     5761 cgggcgagcc gcctccgctt tgggcagggt cagcttcagc accccgtcct tgagcgtcgc
     5821 ttcgatccgc gcctggtcga tcaggttcgt caaacggaac tcgcgacggt acgcgcccgt
     5881 gtcgtactcg cgcagcagca gcacctcgtt cggcccgccg acgtcgtcga ccgagccgtc
     5941 gatcgtcagc acgttgtcgc gcaggtcgat cgcaagaccc tcactcgtga cgcccggcat
     6001 gtcggccaac agcgtgatcg cgccgtcggt ctcgtagatg tcgacgaccg gagtgaaaac
     6061 ccgacccggg cgcgtcgact cggctccgct caccggctgc ttttcccgct gcgtcatctc
     6121 tttcttggtc gcttccttca tgatcgacat gatgatctcc tacctcgcgc gctcaggcgc
     6181 cggtcgtgat cgggatctgc ttcggccggg cctcgaccgc cttgggcagc acgaccgtca
     6241 ggatcccgtc ggtgtagcga gcgtcgacct tcgcggaatc gacccgcgtg ttgagcgaga
     6301 agctgcggcg gaagcgtccc cactcgcgct cgcgacggtg aaagctggtt tcctgttcct
     6361 cctgcgaccg gtggcgctcg ccggagatcg tcaacgtgtc gttctcgacc gtgacgttga
     6421 gattgccggg cgtgcacccc ggcagctcgg cccgcacgta gatcgattcc gggtcctcgg
     6481 agacgttgat cgccggaaag acgccggccg accggtaccg ggtgatctca cccaacgcgt
     6541 cgaacaattc gtccatccct cgccgggccc cgtcccaatc acgaacgatg gacggccagg
     6601 tggggaggga tgggagtctt cgcaacaaca tcgcaaaacc ctccttggtt cgctggtggt
     6661 gtgtcggttg tcggacccgc caggatcgcg cgggcccggc ttcccgttcg ccgagtctgt
     6721 taagacggtc gatcgcgggt gtcaaggcag gtcggccgcc ggcagcggtc ccgaacgatc
     6781 ttcagcgaac gacgatgaac tcgcggcgcg cgagcacgtt gcccgaggcg tcgcgtgcct
     6841 cgaccgccca ctcggcggcc gagcccgggc gcagcgtttt ggcactgtag gtgcgccagc
     6901 tcctaccgcc gagctcgagc gagatccgcg ccgcctcgac gccgtcctcg atccagacgt
     6961 gatcgacgcg gtcgccgcgc cggccgccct cgacgcgggt ccagaaccag acctgcgagc
     7021 cccgctcgaa gcgctcgccc tcgccgatcg gctcgagccg gcgcacgccg gtgccgaccg
     7081 cggcctccgc aacggtcagg gtcggggagc cggccgcgac cgggctcgcc gcggccggga
     7141 cgagggcacg ccccggcgtg ggttcttccg cggccgccgg cgcgggtgcc gcagcggtcg
     7201 gcgcgcgttc gatacggggc ggcgccgacg gacccgcgga gaagaagacc caccacagac
     7261 cgaagctcgc gacggccgtc accacgatcg aagcggcgag aagtcgagga aagggcgacg
     7321 accggccgac cggcggcgcc gccgcggtgc gcttgacgtc ggcgaccgcg tcgattcgcg
     7381 tcgcgagccc gtcgaggcag cgctgacgct cgcggaggta gtgctcgatc atcagctcgg
     7441 cttcgacgcc cagacagtcg gcgtacgtcc ggagcagcgg catcatcgtg gcgtcgtcgg
     7501 gcagcgaggc gaggtcgttc cgctcgagcg ccaacaggcg ttcacgctcg acgccgagct
     7561 tgtcggtcag ccccgcgata gtgaggccac ggccctcccg ttcggacctg agcgtccgtc
     7621 cgaaatcgtc catcgtggcg tttctcctcg ctgtgaactt ggtgaccggt cgagcgaagg
     7681 tcaagcgtcg cccgccgcgg ggttgatggc gatgcggttt tcctctaggc tcgacgagga
     7741 atccacccga acgagccctt ctttactgga gcgaccacca tggcagctgg tacccgtccc
     7801 gatcgatcga tcaacgtcgg cgtcaggcgc ctcgagccga ccggcctgtg gaaccacttc
     7861 gcggatctca acgcggtgcc gcgggcgtcg aaaaaagaac agcgggtgat tcggttcatg
     7921 cgggccttcg gcgagcgcct ggcgctcgag acggtcgtcg acgaggtggg caacgtcgtg
     7981 atccgcaaac cggccagccc gggcatggaa gaccgcctac cgatcgtgat gcagtcgcat
     8041 ctggatatgg tgcaccagaa gaacgccgac accgatttcg acttcgacac cgaggggatc
     8101 gagatggtgg tcgagggcga ttgggttcgt gccgcgggca cgacgctcgg cgccgacaac
     8161 ggcatcgggg tggcgacgat catgacggtc ctcgccgagg acgcgatcgc ccatccgcgg
     8221 ctcgaggcgc tgttcacgat cgacgaagag accgggatga caggggccaa gggactgaag
     8281 ggcggtctgc tgaccgggag aatcctgctc aacctcgaca cggaagcgga cgacgagctg
     8341 acgatcggct gcgcgggagg ggtcgacgtc acggcgaccg gccgctacga cccggagtcc
     8401 acccccgccg ggcaggtcgg cctggagctc gtggtccgcg gcctccaggg cgggcactcc
     8461 ggcatggaga tccacgtcgg gttcgccaat gccaacaagc tgatgaaccg cctgctcgcg
     8521 ggcgtcgtgc gccgtttcgg cgcgcggctg tcgtcgatcg acggcggcgg gctgcgtaac
     8581 gccatcccac gcgagtcgtt cgccatcgtc acggtctcac gggagcggcg cgacgagctc
     8641 gcggcctggg tcgccgccga ggccgagacg atccgtgtcg agtaccgaac gaccgacccc
     8701 gccgccgacg tccgctgcac gccgatcgac gcgccgcgcc aggtcgtccc gaccgagctg
     8761 acacggctcc tgctcggcgc gatcgacggg gtccactccg gcatccaccg gatgagcccg
     8821 gaggtcggcg gcctggtgca gacgtcgaac aacctggcac gcgtgcgggt cgcggacggc
     8881 gaattcaccc tcggctgcct gactcgcagc agcgtggaca gtgaacgcga cgcgctggcg
     8941 cggtcgatcg ccgcggtgct cgagctgacc ggtgcggatc tgtcgacgag cgggagctac
     9001 cccggttggc agcccgcgcc cgagtcggcg atcgtcaagc tgatgtcgtc gatctacaag
     9061 gagatgttcg acgcgccggc cgaggtctcc gcgtgccatg ccgggctcga atgcggcatc
     9121 atcgggacga actaccccgg tctcgagatg atcagcttcg gcccgacgat cctcggtgct
     9181 cactcgccgg acgagcgggt caagatcagc agcgtgcaaa agtactggcg cttcctgctc
     9241 gaaacgttga aacggatccc ggccggcgac tgacgcgcta cagcccgaaa gcgacgatcg
     9301 cgaggcccga cgccgcgatc acgcctccgg cgagcgcctg gctccaccgt tcgagccagc
     9361 gccagcgcaa ccgctcgaac ccgtgcaagc cgagcgtggt caggccgacc atcgtcgcca
     9421 cggtgacgac cgcgaagacg agcgccgtga cgcaggccca gtcccagcga ccgcggcttg
     9481 ccggtaggac gaacagcggg atcaacggct cgcacggacc gaggacgaag atcacgaaca
     9541 acgaccagaa cccggcgtcg cctcgctgcg ccgggaaccg gaagtggcgg tgcgggcggt
     9601 cgccgtggag atgtaggtgg agccgcccgt cgtgggtgtg gggctcgaga ccggatgcgc
     9661 gacgcaaacc cctgcgcatc ccccagaggg cgtaggccag cccgaacccg atcagcagcc
     9721 aggccgcgag gtcgccgcga gcctgttcca ttcgctcgat ccgcccgacg gcgaacccga
     9781 gcagcaaccc gccgccggcc agcgaccacg aggcgacgac gtgtgccgac ccgcaggcgg
     9841 tggtgatcca cgcggtcttg acgcgacccc acccgcgcga ccggccgagc atgatgaacg
     9901 gcaagtagtg atccgggccc agcaccgtgt ggattgcggc gacggcggcc gccgccagaa
     9961 ccagactgaa gcccgctccg tcgccggaca tcggctagcg cgacgacgtc acgggtaggt
    10021 gtaggccggt tgtagaccca gcggcagacc gatcgcccac gaggaagatc gtccagctga
    10081 cgagcagcga gacggtatac gggagcatga gggacgtgat cgtcccgatg ccgctgccac
    10141 gcacccagcg ctgcgcgaag gcgacgacga gcgggaagta gggcatcaac ggcgtgatga
    10201 tgttggtcgt cgagtcaccg acgcggtacg ccgcctgggt cagctctggc gacaggccga
    10261 gctgcatcag aatcgggacg aagatcggag ccaaaagcgc ccacttgccc gaggccgacc
    10321 cgaccaacag gttgacgacc gacgtcagca ggatgatccc gacgatggtg acgaagccgg
    10381 acagaccgag agcgcgcagc aggtcggcac cttcgaccgc gagcaacgcg ccgaggttcg
    10441 accggccgaa cgccgcggtg aactgggcgc agaagaagat catcaccagg tagtagccca
    10501 tcgtctgcat cgacttggtc atcgccacga tcaaatcgcg gtggctccgc acggtgccgc
    10561 cgatgtaacc gtagacgatt gcaggaaaca ggaacagcag gaagatcagc gggacgatcg
    10621 actgcatgat cggtgcgccg aacgatgtga tcgagccgtc cgcggcatgt agcggcgagt
    10681 cggccggcca cgccgccgcc caaagcacga cgccgagcgg cggaaagccg gtgaaggagc
    10741 tgaccatcgt cgacagcgcg gtggccaacg ccgaccccgt cgcctggttc tgcacctcga
    10801 gcatccgatc gccgtcgcgc gccggcacgg taaacgggtg gccggccaac agccacgaga
    10861 cgatccagac cccgaccagc gccaggacga agagcatcgc cggatcgggc agccggttgc
    10921 cggcacgctc gacccggccg agtgcccgat cgatccagcc cgccttaggg ccggcgcccc
    10981 ccggtttcgt cgcgtcgcgc acagccgact tctcccgcag cccgatatgg accgcgccat
    11041 tctacaggcc gcgcacggct cgtcccgctc agagaccggc gatcttccat tcgccgtcgg
    11101 ctgtttcctc gaagaagatc catttcggcc gccgccgctc ccgcacgccg tagatcgacc
    11161 agtaggggcc gacccgcccg acgttctcga tccgactgaa gctcgagacc agcgcacgca
    11221 gcgcgccgat cggcgaggcg tcgggcacgt cgtcgagcgc gccgagcgcc gacgggacga
    11281 agcaggcttc ggccgcgacg cggtccccac gcccgagggc ggccaacagg ctttcccacg
    11341 tgcgcgccgg gtcggtgaac ctcggagcgg cgggagtcgt cggcgcgacg gccgacg
//
LOCUS       DASPGN010000095         5605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1187983, whole genome shotgun sequence.
ACCESSION   DASPGN010000095 DASPGN010000000
VERSION     DASPGN010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5605
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187983"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VD788_02640"
     CDS             <1..323
                     /locus_tag="VD788_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV75188.1"
                     /translation="ELERAKAEQRAARRESARGIGELYFALAEAEARRSVMANEVLPA
                     ARATFDAFRLAFERHAENLNELLDSRHDRTRAEIDHIDSLVDVHRSLAALEAMVGQPL
                     AGEP"
     gene            complement(368..443)
                     /locus_tag="VD788_02645"
     tRNA            complement(368..443)
                     /locus_tag="VD788_02645"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(408..410),aa:Phe,seq:gaa)
     gene            complement(493..1644)
                     /gene="recA"
                     /locus_tag="VD788_02650"
     CDS             complement(493..1644)
                     /gene="recA"
                     /locus_tag="VD788_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXV75189.1"
                     /translation="MSVEQKDKGKSVDLAISQIERQFGKGSIMKLGQRGKFDVPVIPT
                     SSLSIDYALGVGGIPRGRVVEIFGPESSGKTTLALHVVAEAQRAGGLAAFIDAEHALD
                     PEYAGKLGVDVDNLMVSQPDSGEQALEIAEILIRSGALDVVVIDSVAALVPKAELDGE
                     MGDSHVGLQARLMSQALRKLTAIVAKSHTSLIFINQIREKIGVMFGNPETTTGGRALK
                     FYASLRLDIRRIGAIKEGDETIGNRTKLKVVKNKVAPPFRVAEFDILYGEGVSREGDL
                     LDLGVAHNVIDKSGSWYSYGELRLGQGRENARIFLRDNPDLAHEADRKIRTALGLIKP
                     AASAQPTDAATAGETTDAATADATPATQPTPIDKARSSRGRPITRPATP"
     gene            1954..3438
                     /locus_tag="VD788_02655"
     CDS             1954..3438
                     /locus_tag="VD788_02655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HXV75190.1"
                     /translation="MVGRARGAVLLGIDARCVEVEVDLPGGLPTIAAVGLPGSTVREG
                     IDRIRAALRHCGFRLPDGRIVVNLAPADLRKQGAALDLPIATALLIASGQIPPLDDDW
                     LFAGELALDGTVRAVRGALSLALLAARSGRSRVVLPRDNAAEAALVGGVEVHAVGHLN
                     EMRAVVSGQATSIRRADVERALDEPDRAGPDLADIRGQAGARRALEIAAAGAHHLLLS
                     GPPGAGKSMLARRLAGILPPMTIDEALTVTTIHSAAGIVRELVTRRPFRAPHHGISLA
                     GLTGGGPSIRPGEIALACHGVLYLDELTEFKRDVLESLRQPLEDARITIVRVHGAATF
                     PAEFSLVASMNPCNCGFHGSPDGRCSCSPAEIRRYLARLSGPLLDRFDLMVDVPPIEL
                     SALGRAATGECSAVVRERFGADGSNCNARMGPAELDRHAVLDRDARRLLLVAGERMGL
                     SARGFDRVRRVARTLADLEGRETLAVSDVAEALQYRQSATLGRE"
     gene            complement(3632..>5605)
                     /locus_tag="VD788_02660"
     CDS             complement(3632..>5605)
                     /locus_tag="VD788_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75191.1"
                     /translation="YIADTDPLGTNPDRVPQVFAVNRDGSFPIQLTSATSATQATHVN
                     ELSRLDVDISDEGSIVLFKHQPGTFVILDDGTGLREVEAGPVPYLSGNGLHVTSRFGD
                     DAIVVDPWHSLIELSQPGTDPTITSDGSFIYFVDDRLDPTQNPDGGGEIWKSSLDGSI
                     VTPLTATTDVTNEQPIVAGGGSRIAFTRGGTTTYSLRAIDFRGDNEVVLAEWQRSFTE
                     VVAPDITPDGTRIVYNLEGTLMRYDPAEGVHHEVPLDPQVWPEAISITGDGETIVLQS
                     YLDPLGLGNDSGLPQAYAVEADGTGLRVLSGSFVPHPGAWYPVIAVNGSWVVFMSDND
                     LYRVRPDGTELLQITNLGTNGGWQPRLSADGEWVVFAHDGINRMRIDGSGLQKIVPSI
                     QFETYYPDISGDGNQVVWAGETDPVGTNPDGNVEVFHYDVQNDTITQLTDTTAGNAFH
                     PRITEDGAWVYFDSSSPFFDENPQGRNEWFRYEVATGRLERAGGIRRGPSEVYTHFVD
                     QYWHVAVDATGDRAAFAAVGDWAGTNPDYSGELYLADYRGSGRIEASKQAPTLVTWEA
                     DPRYVRYDIIRGDLANVGYDAGTITLGPVACLESASPDTDTRGFEDPAQPGPGEVLFY
                     VFRGTQGVGDAVNDWGRGSGAIERVPASGACSP"
BASE COUNT          834 a         1869 c         1955 g          947 t
ORIGIN      
        1 acgagttgga gcgtgccaag gccgagcagc gtgcggcgcg ccgcgagagc gcgcgcggga
       61 tcggcgagct ctacttcgcg ctggccgaag ccgaggcccg ccgctcggtg atggccaacg
      121 aggtcctgcc cgccgcgcgc gcgacgttcg acgccttccg tctggcgttc gagcgccacg
      181 ccgagaacct gaacgagttg ctcgactcgc gccacgatcg gacgcgcgcc gagatcgacc
      241 acatcgattc gctggtcgac gtccaccgat cgctcgccgc gctcgaggcg atggtcggcc
      301 agccgctggc cggcgagccg tagctcgcga gccggcggcg ggcgagcgat gtggctatcg
      361 gtcaggttgg tgcctcgggg cggagttgaa ccaccgacgc agggattttc agtccctcgc
      421 tctaccgact gagctaccga ggcaaggcct gacgcgagcc ggcggcatgt tgtcacacgg
      481 tccgcgacag cgtcaaggcg tcgccggtcg cgtgatcggc cgaccgcgcg acgaccgagc
      541 cttgtcgatc ggcgtcggct gcgtggcggg cgtggcgtcg gcggtcgcgg cgtcggtggt
      601 ctcaccggcg gtcgcggcgt cggtcggttg cgcactggcc gcgggcttga tcagcccgag
      661 cgccgtgcgg atcttgcggt cggcctcgtg ggccaagtcc gggttgtcgc gcaggaagat
      721 gcgggcgttc tcgcgaccct ggccgagccg cagctcgccg taggagtacc acgagccgga
      781 cttgtcgatc acgttgtggg ccacgccgag gtcgagcaga tccccctcgc gcgagactcc
      841 ctcgccgtac aggatgtcga actcggcgac gcggaacggc ggggcgacct tgttcttgac
      901 gaccttcagc ttggtgcggt tgccgatcgt ctcgtcgccc tccttgatcg caccgatgcg
      961 gcgaatgtcg agccgcaacg aggcgtagaa cttgagcgcc cggccgccgg tcgtcgtctc
     1021 ggggttgccg aacatcacgc cgatcttctc gcggatctgg ttgatgaaga tcagcgaagt
     1081 gtggctcttg gcgacgatcg ccgtcagctt gcgcagcgcc tgcgacatca gtcgggcctg
     1141 caggccgacg tgggaatcgc ccatctcgcc gtcgagctcg gccttgggca cgagcgccgc
     1201 gaccgagtcg atgacgacga cgtcgagcgc gcccgaccgg atgaggatct cggcgatctc
     1261 gagggcctgc tcgcccgagt cgggctgcga gaccatcagg ttgtcgacgt cgacaccgag
     1321 cttgccggcg tattcgggat cgagcgcgtg ctcggcgtcg atgaaggcgg cgagcccgcc
     1381 ggcccgctgg gcttcggcga cgacgtggag cgcgagcgtc gttttgcccg acgattcggg
     1441 gccgaagatc tcgacgacgc gcccgcgcgg aatcccgccg acgcccagcg cgtagtcgat
     1501 cgacagcgac gaggtcggga tcaccgggac gtcgaacttg ccgcgctggc cgagcttcat
     1561 gatcgagccc ttgccgaact gccgctcgat ctgcgagatc gcgagatcga ccgacttgcc
     1621 cttgtctttc tgttccacgc tcatcgagat ctcctggagg tcgttgggga cgggtcgggg
     1681 cgcccgcccc ggcgcacggc cgaagcgtag gcgaaagtga gccaaatgtc aacacgatca
     1741 ggctagccga agacgccgcc cgatgcagga gccgtcccgc cagctcggtt gtcgaccggc
     1801 aaccgcggca tcctcccgca cgaaggtccg atcgggccgt tccgcccgcc cgccgaagct
     1861 ctcgggtcgc ccggtcgagc cggccggcgc gctacaatcc gcgcccggtg ctcggcgggg
     1921 tccccacgac agcctcctcg atcgagcggc tcgatggtcg gacgcgcccg cggtgcggtc
     1981 ctgctcggta tcgacgcccg ttgcgtcgaa gtcgaggtcg acctgccagg agggttgccg
     2041 acgatcgccg cggtcgggtt gccgggctcg accgtgcgcg agggaatcga tcgcatccgc
     2101 gcggcgctcc gtcactgcgg cttccgcctg cccgacgggc ggatcgtggt caacctcgcc
     2161 ccggcggacc tgcgcaagca gggtgcggca ctcgatctgc cgatcgcgac cgctcttctg
     2221 atcgcctcgg gacagatccc gccgctcgac gacgattggt tgtttgccgg ggagctggcc
     2281 ctcgacggca ccgtccgcgc cgtgcgcggc gcgctctcgc tggcgttgct cgccgctcgc
     2341 tcggggcgat cgcgcgtcgt cctcccgcgg gacaatgccg ccgaggccgc gctcgtcggc
     2401 ggagtcgagg tccacgcggt cggccacctg aacgagatgc gcgcggtcgt ctcggggcag
     2461 gcgacgagca tccgccgcgc ggacgtcgaa cgggcgctcg acgagccgga ccgggcgggc
     2521 cccgatcttg ccgacatccg cgggcaggcc ggcgcccgcc gcgcgctcga gatcgcggcg
     2581 gcaggcgcac atcatctgct gctgagcggt ccgcccggcg cgggcaagag catgctcgcg
     2641 cggcgcctgg ccggcatcct gccgccgatg acgatcgacg aagcgctgac cgtcacgacc
     2701 atccacagcg cggccggcat cgtacgcgag ctggtgacgc ggcggccgtt ccgtgcgccg
     2761 catcacggca tctcgctcgc cggtctgacc ggcggcgggc cgtcgattcg tcccggcgag
     2821 atcgcgctcg cctgtcacgg cgtgctctac ctcgacgagc tgacggagtt caaacgcgac
     2881 gtgctcgagt cgttgcgcca gccgctcgaa gatgcgcgaa tcacgatcgt ccgcgtacac
     2941 ggtgcggcga ccttccccgc ggagttcagc ctggtggcgt cgatgaaccc gtgtaactgt
     3001 gggttccacg gaagtcccga cgggcggtgc agctgctcgc cggcggagat ccggcggtat
     3061 ctcgcgcgac tgtccgggcc gcttttggat cgcttcgatc tgatggtgga cgttccgccc
     3121 atcgagctgt ccgcgctcgg gcgtgccgcg acgggggaat gctcggcggt cgtgcgcgag
     3181 cgattcggag ccgacgggtc gaactgcaac gcccggatgg ggccggcgga actggatcgg
     3241 catgcagtgc tcgatcgcga cgcgaggcgg ctgctgctcg tcgccggtga gcgtatgggg
     3301 ctctccgctc gcggattcga tcgcgtgcga cgcgttgcgc ggacgctggc ggatctcgaa
     3361 ggtcgtgaga ccctcgccgt cagcgacgtc gccgaggcgc tgcagtaccg acagagcgcg
     3421 acgttgggga gggagtgagg ctgaagacga tctcgcatgc tgccgtacgc cgtacgccta
     3481 cctgtattcc gcgatgaacg aatagatgtc tccccgtggt ccacctgagt ccggtcgggc
     3541 ggggcagccg acttcaggcg ccaacctgaa ctgctgaacg ggcaggggac gattccgggc
     3601 cgttggacgg tggacgggtc gtcgcgcgcc gctacgggct acacgccccg ctcgcgggga
     3661 cgcgctcgat cgctccgctg ccacggcccc agtcgttcac cgcgtccccc acgccctgcg
     3721 tgccgcggaa gacgtagaac agcacctcgc ccggtcccgg ctgcgccggg tcttcgaaac
     3781 cgcgggtgtc ggtgtcgggt gacgcgctct ccagacacgc cacgggtccg agcgtgatcg
     3841 ttccggcgtc gtatccgacg ttggccaggt cgccgcggat gatgtcgtag cgcacgtagc
     3901 gcgggtcggc ttcccacgtc acgagcgtcg gcgcctgttt gctcgcttcg atgcgaccgc
     3961 tgccacggta gtcggccagg tacaactcgc cgctgtagtc cgggttggtg ccagcccagt
     4021 caccaacggc cgcaaacgcg gcacgatcgc ccgtggcgtc gaccgccacg tgccagtact
     4081 gatcgacgaa gtgtgtatac acctcggaag gaccgcgacg gatgccgccg gcgcgctcga
     4141 gtcttccggt cgcgacctcg tagcggaacc attcgttgcg accctgtgga ttctcgtcga
     4201 agaagggact gctcgaatcg aagtagaccc acgcaccgtc ctcggtaatc cgcgggtgga
     4261 acgcgtttcc tgcggtcgtg tcggtcaatt gcgtgatcgt gtcgttctgg acatcgtaat
     4321 gaaacacctc gacgttgccg tcgggattgg taccgaccgg atcggtttcg ccggcccaga
     4381 cgacctggtt gccgtcgccg gagatgtcgg gatagtacgt ttcgaactgg atggacggaa
     4441 cgatcttctg caggcccgac ccgtcgatcc gcatccggtt gatgccgtcg tgagcgaaga
     4501 ccacccattc gccgtcggcg gagagacgcg gctgccagcc accgttggtc ccgagattcg
     4561 tgatctgcag cagctccgta ccgtcggggc gtacacggta caaatcgttg tccgacatga
     4621 acacgaccca gctgccattg accgcgatga ccggatacca cgcaccgggg tggggcacga
     4681 acgacccgga caacacccgc aatcccgtac cgtcggcctc gacggcgtac gcttggggga
     4741 ggccggagtc gttgccgagg ccgagcggat cgagatagga ctgcaggacg atcgtttcgc
     4801 cgtctccggt gatcgatatc gcttccggcc agacctgggg gtccaacggt acttcgtggt
     4861 ggacgccctc ggcgggatcg taccgcatca gcgtgccttc gaggttatag acgatgcgcg
     4921 tgccgtcggg agtgatgtcg ggtgcgacga cttccgtgaa acttcgctgc cactcggcga
     4981 gcaccacctc attgtctccc ctgaaatcga tggcgcgcag gctgtaggtg gtggtcccgc
     5041 ctcgggtgaa cgcgatgcgc gagccgccac ccgccacgat cggctgctcg ttcgtcacgt
     5101 cggtcgtcgc cgtgagaggc gtgacgatcg acccgtcgag actcgatttc catatttctc
     5161 caccaccgtc ggggttctgg gtcgggtcga ggcggtcgtc gacgaagtag atgaacgatc
     5221 cgtcggacgt gatcgtcggg tcggtaccgg gctgcgacag ttcgatcagg gaatgccacg
     5281 gatcgaccac gatggcgtca tcaccgaatc gcgacgtcac gtgcaggccg ttccccgaaa
     5341 gatacgggac cggtcctgct tcgacctccc ggagacccgt gccgtcgtcg aggatcacga
     5401 acgtgcccgg ctgatgtttg aacagcacga tcgacccctc gtcgctgatg tcgacatcca
     5461 acctcgacaa ctcgttgacg tgggtcgcct gtgtcgcgga cgtggcggag gtcagctgga
     5521 tcgggaagct gccatcgcgg ttgacggcga acacctgcgg tacgcggtcg ggattggtcc
     5581 cgagcgggtc cgtgtcggcg atgta
//
LOCUS       DASPGN010000096         7183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_953754, whole genome shotgun sequence.
ACCESSION   DASPGN010000096 DASPGN010000000
VERSION     DASPGN010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953754"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="VD788_02665"
     CDS             complement(<1..1020)
                     /locus_tag="VD788_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75192.1"
                     /translation="MRTPPARRLRPIAALSVTLCAALGMVFAPTAADEPDAPPAWMAL
                     GISLDSADAVPDGIGDDLAGFRVRLVVDWNRIERPVGVYDWSRVADRVDAVLARGAEV
                     VLCLTGSHLDHVAGGVFPDPADVGAIDAWLAFVRAAVTRFAGRIEAIEVWDASTGSAI
                     DPEDYAYVLKTTALAARAEASARGATIRIAQLAVSAAEIEWQRRLWQRDVAAYIDILP
                     LDVRTEGLDRGIDSRLATMMAENLRHPPAAEVWAHVGAGIGFGPWLPVTDALRALAAG
                     VPVAIVRPPADPQPRGEVAGWLAGTHRLVRRGFAPAPLGAVRFEGAQGGELRGARAIA
                     AFLADE"
     gene            1127..1942
                     /locus_tag="VD788_02670"
     CDS             1127..1942
                     /locus_tag="VD788_02670"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HXV75193.1"
                     /translation="MKTSKIITLTTDFGQRDSFVGQMKGAALSVDPEARLVDLCHGVP
                     PHDVRAGSYLLETGYNAFPDGTVHVVVVDPGVGTDRRLVAVHAGRHLFLAPDNGVLSR
                     VLAREKIVAAHLLQNPTLYRSPVTPTFEGRDVLAPVAAWITRGVELQRLGPPAGQLVT
                     LEPTGPRVVPGRPCRVAVVHVDHFGSVVLDMTATQLTALLGHAPDRDSPLTLRCEGGI
                     VSRFVRTYGEADGVEPFFLINSTGYLEIACRAGRADERLALRAGVHPELTVGD"
     gene            2006..3388
                     /locus_tag="VD788_02675"
     CDS             2006..3388
                     /locus_tag="VD788_02675"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXV75194.1"
                     /translation="MPLFGTLSTMPLTDLLQWLGSARQTGTLQIERDKVSKAIVFHDG
                     QVIGCSSDDPPQRLGQFLITQGKISEEQLRHALMIKESTGKHLGMTLVEMGALSPAEL
                     SSHLEAKAEETIFSLFDWEDAVFRFQHGIRDEANVFPISLRVEDVLLRGLKRFDEIQR
                     IREVLYDARIVLRRTDKRPPEAVLRNAMARSLFDTVDGDRTLAEIRLHVHGSEYVVHK
                     FLFELYQNGYVTVAGIKKIEPVSPGVPHAPVVAPPPPAAAIDRPPGAAVDHRPSVPKP
                     SAAGSAPAERPAPPPTDRPAAEPRPSATRETHVFADVEIPTELESADESEAYQLTRKL
                     EGARGHMRKGEFEAALVILEHVSSQYPGDESLRRLTAEAESAFIDKAYRYYFAPSKIP
                     RLTRPIESMATDAMSPTEFFLLSRIDGSWNVKSIVQVAPLREADTLRALKRMREQGLI
                     ELQDAEGSGD"
     gene            complement(3390..3776)
                     /gene="acpS"
                     /locus_tag="VD788_02680"
     CDS             complement(3390..3776)
                     /gene="acpS"
                     /locus_tag="VD788_02680"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HXV75195.1"
                     /translation="MIVGLGIDLVEVRRLAAALERHGGRFASRVYTARERADCATRGD
                     RVLALAARFAAKEACLKALGTGWRDGLGFRQIEVTRGPTGAPRVRLTGAAAARAGQLG
                     VDAIHVSLTHQATVAAAVVVLERTGA"
     gene            3957..5540
                     /locus_tag="VD788_02685"
     CDS             3957..5540
                     /locus_tag="VD788_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXV75196.1"
                     /translation="MSVDDGILFERLGANGLKRVLDDLLDQRKLVRLANACSLEYPGM
                     RAQSLKRTRLIADIVERAFNDRATMTAVSAAVHKETRDASLRWNRLADDEKAKRIGDD
                     KFLRRKGNLGLHLFLLASTDADGIESLLARQPLFRSGDGDAATKDEGETRPPREQVRL
                     EKKLAELNKKHQHLEAQLVKSRETERAFKRDLIQRKGELAEARMLVERLRGELIEMQQ
                     AARRAAAELPATPVIQEAIDKLDRAVRRLANEQKKTVHRLDKLGEAGDANGRAEAVRL
                     DADQIEPLVTAVDAMRKELTAVRRDRKKESSAVAGAIDALREQLDALRDGSGGGGRRR
                     SSSPKRKGEPDRVGVFIDVQNMYYGARQLKGKLDFDALRQAAVRDRRLIQAVAYVVES
                     NEIDQSGFIKLLEQRAIEVRRKTLQVRADGSMKGDWDMELALDILDAAPDLDVVVLVS
                     GDGDFTSLVKHVKTMGPRVEVIGFPRTTAKSLVGAADHFQPLDRKFMIYSGRSRTRGG
                     RLPAGDHDEADGGVDKKTA"
     gene            complement(5553..6287)
                     /locus_tag="VD788_02690"
     CDS             complement(5553..6287)
                     /locus_tag="VD788_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXV75197.1"
                     /translation="MTTGARDLVFIGDVHLERVDPSLADFLRLLDRLARTASRLVLMG
                     DLFNVWIAGDGIEQPHQRAVAERLRGLRRAGLVVRYLEGNRDYRVGRHHSGDAFDDVS
                     DVGLVERFGGRSIWAAHGDLVNLDDRPYRLWRRVSRSSAVWAVFGLLPRARRLRVAES
                     IERRMRATNLSHKREFPERRVRRYAEQQARAGHDTVVLGHFHVEREIAIDRPPGPLRV
                     WVLPEWRESRRHLVARADGRMAFVDS"
     gene            complement(6284..>7183)
                     /gene="tgt"
                     /locus_tag="VD788_02695"
     CDS             complement(6284..>7183)
                     /gene="tgt"
                     /locus_tag="VD788_02695"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:NF013835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXV75198.1"
                     /translation="RALGGLHRFMNWDGPILTDSGGFQIFSLAELRRIEEDGVRFRSH
                     VDGSAHLLSPERSMEIQAALGSDIAMVLDECPPGDADRERLEQAVGRTTRWARRSREH
                     YDGPGVLFGIVQGGVRADLRERSAREITALDFPGYAIGGVSVGEPVERIVDTVRTTAE
                     LLPADRPRYLMGMGMPLDLLRAVAAGVDIFDCVIPTRHARNGSLFTSTGRINIKRREF
                     RDDDRPLDAACRCEACRGYSRGYLRHLFLAREILAARLHTIHNLTFYQGWMARMRRAI
                     AERAFDRLLAEASTDSRPSGDGV"
BASE COUNT         1191 a         2511 c         2470 g         1011 t
ORIGIN      
        1 ctcgtcggcg aggaaggcgg cgatcgctcg cgcgccacgc agctctcctc cctgcgctcc
       61 ctcgaagcgc accgcgccga gcggcgccgg cgcgaagccc cggcggacca gtcggtgcgt
      121 gccggccagc catcccgcga cttccccgcg cggctgcgga tcggcgggcg ggcgcacgat
      181 cgcgaccggc actccggccg cgagcgcgcg cagcgcgtcg gtcacgggaa gccacgggcc
      241 gaagccgatg ccggctccga cgtgggccca gacctcggcc gcgggcgggt gccgcaggtt
      301 ctccgccatc atcgtcgcca atcggctgtc gataccccga tcgaggccct cggtgcgtac
      361 gtcgagcggc aggatgtcga tgtaagccgc gacatcgcgc tgccacagcc gtcgctgcca
      421 ttcgatctcc gcggcggaga cggcgagttg cgcgatgcgg atcgtcgctc cgcgggcgct
      481 ggcttcggcc cgcgcggcga gcgccgtggt cttcagcacg taggcgtagt cctcggggtc
      541 gatcgcggac ccggtcgagg cgtcccacac ctcgatcgct tcgatccggc cggcgaaccg
      601 ggtcacggcg gcgcggacga acgcgagcca cgcgtcgatc gcgccgacgt cggccggatc
      661 ggggaagacc ccacccgcga cgtgatcgag gtggctcccg gtcaaacaga gcaccacctc
      721 ggccccgcgc gcgagcaccg catcgacccg atccgcgacc cgagaccagt cgtagacgcc
      781 gaccggccgc tcgatgcggt tccaatcgac gaccagccgc acgcgaaagc ccgcgaggtc
      841 gtcaccgatc ccgtcgggca cggcgtcggc ggaatcgagc gagatgccga gcgccatcca
      901 cgccggcggg gcgtccggct cgtcggcggc tgtcggcgcg aacaccattc cgagcgcggc
      961 gcatagcgtg acgctgagcg cggcgatcgg gcggagacgt cgggccgggg gggtacgcat
     1021 cactgctcga cggagcattg ttcgtcagcc ggcgggtggt gtcaatccga gcttcgcttg
     1081 acacgcggcg gtcaaagtgt catctaactt gctgagggga gatccgatga agacgtcgaa
     1141 gatcatcacg ctcaccaccg acttcggtca gcgcgacagc ttcgtcggcc agatgaaagg
     1201 cgccgcgttg tcggtcgatc cggaggcgcg actggtcgat ctgtgccacg gtgtaccgcc
     1261 gcacgacgtt cgtgcgggat cgtatctgct cgagaccggc tacaacgcct ttcccgacgg
     1321 aaccgtccac gtggtcgtcg tcgatccggg tgtcggcacc gaccggcgat tggtcgcggt
     1381 gcatgccggc cgccacctgt tcctggcacc cgacaacggt gtgctgtcgc gcgtgctggc
     1441 acgcgagaag atcgtcgccg cccatctgct gcagaacccg accctgtacc gcagcccggt
     1501 gacgccgacg ttcgagggac gggacgtgct ggccccggtg gcggcctgga tcacgcgggg
     1561 cgtggagttg cagcgcctcg gtccgccggc cggccagttg gtgacgctcg agccgaccgg
     1621 gccgcgggtc gtcccggggc ggccgtgccg ggtggccgtg gttcacgtcg accatttcgg
     1681 cagcgtcgtg ctggacatga ccgcgacgca gctgacggcg ctgttggggc acgccccgga
     1741 ccgtgactcg ccgttgacac tgcgctgcga aggcggcatc gtctcgcgtt tcgtccgcac
     1801 ctacggcgag gccgacgggg tcgaaccgtt cttcctgatc aactccaccg gttacctcga
     1861 gatcgcctgc cgcgcggggc gtgccgacga gcggctcgcg ctgcgggccg gggtccaccc
     1921 tgagctgacc gtcggcgact gaggtcccgt attccttgcc ccgatccctg cgtggcctag
     1981 attcgactgc gaaagggaga cgcggatgcc gctgttcgga acgctgagca cgatgccgct
     2041 cacggacctg ctccagtggc tggggtcggc gcgccagacc ggcacgctcc agatcgagcg
     2101 cgacaaagtc agcaaagcga tcgtgttcca tgacggccag gtcatcggct gctcgtcgga
     2161 cgacccgccg caacgactgg gtcagttcct gatcacccag ggcaagatct ccgaggaaca
     2221 gctgcggcac gcgctgatga tcaaggagag caccggcaag cacctgggca tgactctggt
     2281 cgagatgggt gcgctgtcgc cggcggagct gtcgtcgcac ctcgaggcca aggccgagga
     2341 gacgatcttc agtctgttcg actgggagga cgcggtgttt cgcttccagc acggtatccg
     2401 tgacgaggcg aacgtcttcc cgatcagcct gcgggtcgag gacgtgctgc tccgcgggct
     2461 caagcgcttc gacgagattc agcggatccg cgaggtgctc tacgatgcgc ggatcgtcct
     2521 ccggcggacg gacaagcggc cgcccgaggc cgtcctgcgc aacgcgatgg ctcgctcgtt
     2581 gttcgacacc gtcgacggcg accggacgtt ggccgagatc cggctccacg tgcacggctc
     2641 ggagtacgtc gtccacaagt tcctgttcga gctgtaccag aacggctacg tcaccgtcgc
     2701 cgggatcaag aagatcgagc cggtttcccc cggcgtgccg cacgcacccg tcgtcgcccc
     2761 cccgccgcca gccgcggcga tcgaccggcc gcccggcgcg gcagtcgatc atcgcccgtc
     2821 ggtcccaaaa ccgtccgccg ccggctcggc tccggcggag cgcccggcgc cgccgccgac
     2881 cgaccgaccg gcggccgagc cccggccgag cgcgacgcgc gagacccacg tcttcgccga
     2941 cgtcgagatc ccgaccgagc tcgaatccgc cgacgagtcg gaagcgtacc agttgacgag
     3001 gaagctcgag ggcgcccgcg gtcacatgcg caagggcgag ttcgaggcgg cgctcgtgat
     3061 tctcgagcac gtctcgagcc agtacccggg tgacgaatcg ctacgccgat tgaccgccga
     3121 ggccgagtcg gcgttcatcg acaaggctta ccgctattac ttcgcgccgt cgaagatccc
     3181 gcgcctgacc cgaccgatcg aatcgatggc gaccgacgcg atgtcgccca ccgagttctt
     3241 cttgttgagc cggatcgacg ggagctggaa cgtgaaatcg atcgtccagg tcgcgccgct
     3301 gagggaggcg gacacgctac gggcgctcaa gcggatgcgc gaacagggtc tgatcgagct
     3361 gcaggacgcc gagggatcgg gcgactaggc taggcgcctg tccgctcgag aacgacgacg
     3421 gcggcggcga cggtcgcctg atgggtcagg ctgacgtgga tcgcatcgac gccgagctga
     3481 ccggcccgcg ccgcggctgc accggtgaga cggacccggg gcgcgccggt cgggccgcgc
     3541 gtcacctcga tctgtcgaaa acccagtccg tcccgccagc cggtcccgag cgccttgaga
     3601 caggcctcct tggccgcgaa ccgggcggcc agcgccagca cgcggtcgcc gcgggtcgcg
     3661 cagtcggccc gttctcgcgc cgtgtagacc cgtgacgcga agcgtccacc gtgccgttcg
     3721 agcgcggcgg ccaggcgcct gacctcgacg aggtcgattc ccagaccgac gatcatgcaa
     3781 ccacccagtt gcgctttgcc gcgcgccccc ggcgcgccct agctttgcct gcgccgtctc
     3841 gcggtccggg gggcgcgggt cgaatggtat aataggccga actcgagtcg tcggagaaaa
     3901 cacgccaacc tcatgacacg aaagcattta ttggcgaaga tcgggagccg cttacgatga
     3961 gcgtcgacga cgggatcctc ttcgaacggc tcggtgccaa cggcctgaag cgcgtgctcg
     4021 acgacctgct cgaccaacgc aaattggtcc gcctggccaa cgcgtgcagc ctcgagtacc
     4081 cgggaatgcg cgcgcagtcg ctcaagcgaa cgcggctgat cgccgacatc gtcgaacggg
     4141 ccttcaacga ccgcgcgacg atgaccgccg tctccgcggc cgtccacaag gagacgcgcg
     4201 acgcgtcgct ccgctggaat cgactggccg acgacgaaaa ggcgaagcgg atcggcgacg
     4261 acaagtttct ccgtcgcaag ggcaacctgg ggctgcacct gttcctgctg gcgagcaccg
     4321 acgccgacgg catcgagagc ctgctggccc ggcaaccgct gttccgcagt ggcgacggcg
     4381 acgcagcgac caaggacgaa ggcgagaccc gtcccccacg cgagcaggtt cgcctggaga
     4441 agaagctcgc cgagctcaac aaaaagcatc agcacctcga ggcacagctg gtcaagagcc
     4501 gtgagaccga gcgcgcgttc aaacgcgatc tgatccagcg caagggcgag ctggccgagg
     4561 cgcggatgct ggtcgagcgc ctgcgcgggg agctgatcga gatgcagcag gctgcacgac
     4621 gcgcggccgc cgagctcccg gccaccccgg tcatccagga ggcaattgac aagctcgacc
     4681 gcgccgttcg ccggctggcg aacgaacaga aaaagaccgt ccaccgactg gacaagctcg
     4741 gcgaggcggg cgacgccaac ggtcgcgcgg aggcggtgcg gctcgacgcg gaccagatcg
     4801 agccgctggt cacggcggtc gacgcgatgc gcaaggagct gaccgccgtg cggcgcgatc
     4861 ggaaaaagga atcgagtgcc gtcgccggcg cgatcgacgc gctgcgcgag cagctcgacg
     4921 cgttgcgcga cggatccggc ggcggcggac gccgccggag ctcgtccccc aagcgcaagg
     4981 gcgagcccga ccgcgtcggg gtgttcatcg acgtccagaa catgtactac ggcgcgcgcc
     5041 agctcaaggg caagctcgat ttcgatgcat tgaggcaggc cgccgtcagg gaccggcggc
     5101 tgatccaagc cgtggcctac gtcgtcgagt cgaacgagat cgaccagtcg gggttcatca
     5161 agctgctcga gcaacgggcg atcgaggtgc ggcgcaagac gttgcaggtc cgggcggacg
     5221 gctcgatgaa gggcgactgg gacatggagc tcgcactcga catcctcgac gccgcgccgg
     5281 atctcgacgt cgtcgtgctg gtctcgggcg acggcgactt cacctcgctg gtcaagcacg
     5341 tcaagacgat gggaccgcgg gtcgaggtga tcggcttccc gcggacgacg gccaagtcgc
     5401 tcgtcggcgc tgccgatcac tttcagccgc tcgatcgcaa gttcatgatc tactccggcc
     5461 ggagccgcac gcgcggcggc aggctaccgg ccggagatca cgacgaggcc gacggcggcg
     5521 tcgacaagaa aacggcctga tcggaccgcg gctcacgagt cgacgaacgc catccgcccg
     5581 tcggcgcgcg ccacgagatg gcgccgggac tcgcgccact ccggcagcac ccagacccgc
     5641 agcggcccgg gcggccgatc gatcgcgatc tcccgctcga cgtggaagtg tcccagtacg
     5701 accgtgtcgt gtcccgcgcg cgcctgctgt tcggcgtacc gtcgaacccg cctctcggga
     5761 aactcgcgct tgtggctcag gttggtcgcg cgcatccgtc tttcgatcga ctcggcgacc
     5821 cgcaaccgcc gcgcccgcgg cagcaggccg aacaccgccc acaccgcgga cgagcggctg
     5881 acccggcgcc acagccggta cggccgatcg tcgaggttga ccaggtcgcc gtgcgcggcc
     5941 cagatgctgc ggccgccgaa tcgttcgacc aggccgacgt cgctgacgtc gtcgaaggcg
     6001 tcgcccgagt ggtgccggcc gacgcggtag tcgcggttgc cctcgagata gcgcacgacc
     6061 agccccgccc gccgcagccc ccgcaaccgc tcggcgacgg cccgttggtg cggctgctcg
     6121 atgccgtcgc cggcgatcca gacgttgaac aggtcgccca tcagcacgag gcgcgaggcc
     6181 gtccgcgcga gtcggtcgag caaccggaga aaatcggcaa gcgacggatc gacgcgctcg
     6241 aggtgaacgt cgccgatgaa aaccaggtcg cgagcgcccg tggtcacacc ccatcccccg
     6301 acggtcggct gtccgtcgac gcttcggcca gcaggcgatc gaacgcccgc tcggcgatcg
     6361 cccggcgcat gcgcgccatc cagccctgat agaacgtcag attgtgaatc gtatgcagcc
     6421 gcgcggcgag gatctcgcgg gccaggaaca ggtgtctgag atagccccga ctgtagccgc
     6481 gacacgcctc gcagcgacag gccgcgtcga gcggccggtc gtcgtcgcgg aactcgcgcc
     6541 gcttgatgtt gatccgcccg gtcgaggtga acaacgaacc gttgcgggcg tggcgcgtcg
     6601 gaatcacgca atcgaagatg tcgacgccgg cggcgaccgc gcgcagcagg tcgagcggca
     6661 tgcccattcc catcagataa cgcggtctgt cggccggcag cagctcggcc gtcgttcgca
     6721 ccgtgtcgac gatgcgctcg acgggctccc cgaccgaaac tccgccgatc gcgtagcccg
     6781 ggaagtcgag cgccgtgatc tcgcgcgcgc tccgctcccg cagatcggcg cgcactccac
     6841 cctggacgat tccgaacagc acgccgggac cgtcgtaatg ttcgcgactg cggcgggccc
     6901 aacgggtcgt gcgaccgact gcctgctcca ggcgctcgcg gtcggcgtcg cccggcgggc
     6961 actcgtccag aaccatcgcg atgtccgaac ccaacgcggc ttggatctcc atgctgcgct
     7021 ccggcgacaa caggtgcgcc gagccgtcga cgtgcgaccg gaatcgaacg ccgtcctcct
     7081 cgatccgtcg cagctcggcc agcgagaaga tctggaaccc gccggagtcg gtcaggatcg
     7141 gaccgtccca gttcatgaaa cggtgtagac caccgagcgc gcg
//
LOCUS       DASPGN010000097         4481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_720277, whole genome shotgun sequence.
ACCESSION   DASPGN010000097 DASPGN010000000
VERSION     DASPGN010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720277"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1460
                     /locus_tag="VD788_02700"
     CDS             195..1460
                     /locus_tag="VD788_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HXV75199.1"
                     /translation="MPGRFDIEAVRALYPALGRERDGKPLVYFDGPAGSQVPQRVADA
                     VRHYLLEHNANSGGAFVTSHETDDGLAAARRAVADFIGTADADGVAFGPNMTTLTLSL
                     ARAFARTWGRDAEVLVTRMEHDANYTPWVQAARDAGATVRVVEIRRDDCTLDLDDLGD
                     KLGPRTKLVAVGAASNAVGTINPVKRIGELAHAVGAMVFVDAVHYAPHAAIDVTDWDC
                     DFLVCSAYKFFGPHVGVLWGKPGPMAELPVYKLRPSSNRLPDRWMTGTQNHEGIAGTL
                     AAIDHLADLGRATGLPATDRRGALRAAFETIVAYERGLAGEMLDALATLDGVRLWGIG
                     DRTRLGERVPTFAVTHRRLPPAEIARHLADRGIFVWDGNYYALPLTEALGVEPDGMVR
                     IGLLHYNTSAEIDRLIDGLRVLGNPRPSS"
     gene            complement(1490..2365)
                     /locus_tag="VD788_02705"
     CDS             complement(1490..2365)
                     /locus_tag="VD788_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75200.1"
                     /translation="MNEAVKRLEQFDTQTRYTATVVTSERLTDEESAVEVRELVLDVD
                     HPRFGYRVGQSIGVLAPASPAFGQRFHFRLYSIADLPATGAGGLPRIKIAVRRCDYID
                     EYSGERYPGVASNYLCDLRPGNTLTISGPYGPAFEVPAEHDANLILIGSGTGIAPFRA
                     FVKAIYHDVPDWTGRIWLFYGARSGLELFYMNRRRDDFAQYYDKETFEAFRALSPRPN
                     WADPIAWDRAIAERGIELWEMFSDPKTYVYVAGLEKMRDQLDAIFATIAGSKERWQRR
                     KAELAAGRRWVELLY"
     gene            complement(2362..2577)
                     /locus_tag="VD788_02710"
     CDS             complement(2362..2577)
                     /locus_tag="VD788_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75201.1"
                     /translation="MWRTYLTAIAGIALLTAGWAVVQTAWRRVFAPDCPDRDVLAGRG
                     GCRGGGCAHDCDGRYDGRANAIEEERR"
     gene            complement(2582..3163)
                     /locus_tag="VD788_02715"
     CDS             complement(2582..3163)
                     /locus_tag="VD788_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HXV75202.1"
                     /translation="MNGDPLWTIFINAALINNFVLAYFLGICPFLGVSGKLETATRMG
                     GAVTFVMLVSSACAYGINLLLTAIGAPYLRLISFILVIASTVQLVEMFIRKVSPALFR
                     ALGIFLPLITTNCAILGLALFQTNKGYGFVQCIVYALGAGSGFTLALVLMAGLRQRLE
                     LASVPGLVRGTALALTLAGLLSIAFMGFAGLGG"
     gene            complement(3160..3816)
                     /locus_tag="VD788_02720"
     CDS             complement(3160..3816)
                     /locus_tag="VD788_02720"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HXV75203.1"
                     /translation="MTSLPTSTPDDRATDELVKGLWRDNPVFVQVLGMCPVLAVSNTA
                     RNALAMGLATLFVLVMSNVVVSSLRRLIPRQVRIATFILVIASFVTVVDYAIQAISLE
                     LHKALGAFISLIVVNCVILGRAEAFASKNGIARSALDGLGMGTGFVVALLCLGGVREL
                     LGSGSLFGVRVVHAGFQPWVMMILPSGGFFTLAGWLLLINWLKRGAGQRVAAPTGAGR
                     "
     gene            complement(3821..>4481)
                     /locus_tag="VD788_02725"
     CDS             complement(3821..>4481)
                     /locus_tag="VD788_02725"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HXV75204.1"
                     /translation="PPLAAWPMYRAMVGVGVICGLSIVTVFELTRPVIERNRAAALER
                     AIFDVLPGAATSRPFRLDGSNRLVVADGEADAGGPRIYAGYDAAGLLVGAAIETQGMG
                     YQDVIRVLYGYSPEHAAVIGIRVLESKETPGLGDRIETDPEFLANFARLDARLTADGS
                     AIAHPIEAVKHGRKDRQWQVDGITGATISSVAIADMLRRSTADWVPIIQRDLAALRGD
                     G"
BASE COUNT          747 a         1561 c         1497 g          676 t
ORIGIN      
        1 gtcaccgacg ccgacggcga gctgctgacc gcgaccgagg tcttcgtcac gccgagcgag
       61 ctcggcgtcg gcgaccacgc cgcgttcgag gccagtttcc cggaccccca gcagagcgtc
      121 cgcatcctct tcgagctgaa ctgggtctcc tgactcccgt tgaccgcgcg gcccgggcgt
      181 gtgagactcc gctcatgccg ggtcgattcg acatcgaagc ggtgcgcgcg ctctatcccg
      241 cgctcgggcg cgagcgcgac ggcaagccgc tcgtctactt cgacggtccg gcgggaagcc
      301 aggttccgca gcgcgtcgcg gacgccgtcc ggcactatct gctcgagcac aacgccaaca
      361 gcggcggcgc cttcgtcacc agccacgaga ccgatgacgg tctggcggcg gcgcgccgag
      421 ccgttgccga cttcatcgga acggccgatg ccgacggcgt ggcgttcgga ccgaacatga
      481 ccacgctcac attgtcgttg gcccgtgcct tcgcgcggac ctggggccgg gacgccgagg
      541 tgctggtcac gcggatggag cacgacgcga actacacccc gtgggtccag gcggcgcgag
      601 acgccggcgc gacggtgcgc gttgtcgaga tccggcgcga cgattgcacg ctcgacctcg
      661 acgacctggg cgacaagctc ggaccgcgta cgaagctcgt cgccgtcggc gccgcatcga
      721 acgccgtcgg tacgatcaac ccggtcaaac ggatcggcga attggctcac gccgtcggcg
      781 cgatggtctt cgtcgacgcg gtgcactacg caccgcacgc ggcgatcgac gtgaccgact
      841 gggattgcga cttcctcgtc tgctcggcct acaagttctt cggtccgcac gtcggcgtgc
      901 tgtggggcaa gccggggccg atggccgagc tgccggtcta caagctccgc ccgtcgtcga
      961 accgactccc ggatcgctgg atgacgggga cacagaatca cgaagggatc gccggtacgc
     1021 tcgccgcgat cgaccacctg gccgatctcg gccgggcgac cggtctgccc gcgacggacc
     1081 ggcgcggcgc gcttcgcgcc gcgttcgaga cgatcgtcgc ctacgagcgt gggctggcgg
     1141 gcgagatgct ggatgcgctc gcgacgttgg acggtgtccg gttgtggggc atcggcgatc
     1201 ggacccggct cggcgagcgc gttccgacct tcgccgtcac gcaccggcgg ctgccgccgg
     1261 cggagatcgc gcgtcatctc gccgatcggg gcatcttcgt ctgggacggc aactactacg
     1321 cgttaccgct gaccgaggcg ctcggggtcg agccggacgg gatggtgcgg atcggtttgc
     1381 tgcactacaa cacgtcggcg gagatcgacc ggctgatcga cggcctgcgg gtgctcggca
     1441 acccgcggcc ctcgtcctga ctcgtcgtcg aagatcgtcg ttacctcccc tagtacagca
     1501 actcgaccca acgtcgaccc gccgccagct cggccttccg ccgctgccat ctctccttcg
     1561 acccggcgat cgtcgcgaag atcgcgtcga gctggtcgcg catcttctcg agtccggcga
     1621 cgtagacgta ggtcttgggg tcgctgaaca tctcccacag ctcgatgccg cgttcggcga
     1681 tcgcgcgatc ccaggcgatc gggtcggccc agttggggcg ggggctgagc gcgcggaacg
     1741 cctcgaacgt ctccttgtcg tagtactggg caaagtcgtc ccgccgccgg ttcatgtaga
     1801 acagctcgag accgctgcgc gcgccataga acaaccagat cctgccggtc caatcgggga
     1861 cgtcgtggta gatcgccttg acgaaggcgc gaaacggtgc gatccccgtg ccgctgccga
     1921 tcaggatcag gttggcgtcg tgctccgccg gaacctcgaa cgccgggccg tacggcccgc
     1981 tgatcgtgag cgtattgccc ggccgcaggt cgcacaggta gttggaggcc acgcccggat
     2041 agcgctcgcc gctgtactcg tcgatgtagt cgcagcgacg gacggcgatc ttgattcgtg
     2101 gcagtccgcc cgcgccggtc gcgggcagat cggcgatgct gtacagacgg aagtgaaacc
     2161 gctggccgaa ggcgggcgac gcgggcgcca gcacgccgat gctctggccg acccgatagc
     2221 cgaaacgcgg atgatcgacg tcgagcacca gctcgcggac ctcgaccgcc gactcttcat
     2281 cggtgagtcg ttcgctggtg acgacggtcg ccgtgtaacg tgtttgcgtg tcgaactgct
     2341 cgagccgctt gaccgcttcg ttcatcgtcg ttcctcctcg atcgcgttcg cgcgaccgtc
     2401 gtaccgtcca tcgcaatcgt gcgcacagcc cccgccgcgg cagccgccgc ggccggcgag
     2461 cacgtcgcga tccgggcaat ccggcgcgaa cacgcggcgc cacgccgtct ggaccacggc
     2521 ccacccggcc gtcaacagtg cgatgccggc gattgccgtc aggtaggtcc tccacattgt
     2581 ctcagcctcc caatccggcg aatcccatga acgcgatcga cagcagcccg gccagcgtca
     2641 gcgccagcgc ggtcccgcgg acgagacccg ggaccgatgc caactcgagc cgctggcgca
     2701 ggccggccat caacaccagc gccagcgtga agccgctgcc ggcgcccagc gcgtagacga
     2761 tgcactggac gaatccgtag cccttgttgg tttggaacag cgcgagaccc aggatcgcgc
     2821 agttggtggt gatcagcggc aggaagatcc cgagggcgcg gaacagcgcc gggctgacct
     2881 tgcggatgaa catctcgacg agctgcaccg tcgatgcgat caccaagatg aacgagatca
     2941 accgcaggta gggcgcaccg atcgccgtca gcaacaggtt gatcccgtag gcgcacgccg
     3001 agctgacgag catgacgaaa gtgacggccc cccccatccg cgtcgcggtc tcgagttttc
     3061 cggatacgcc gagaaacgga cagatgccga ggaagtaggc caacacgaag ttgttgatca
     3121 acgcggcgtt gatgaagatc gtccacaacg gatcgccgtt catcggcccg cccccgtcgg
     3181 cgcggcgacc cgctggcctg cgccgcgctt aagccagttg atcagcagca gccaaccggc
     3241 caaggtgaag aaccccccgc tcggcaggat catcatcacc cacggctgga acccggcgtg
     3301 cacgacccgc acgccgaaca gcgacccgct gccgagcagc tcgcggaccc cgcccaggca
     3361 cagcagcgcg acgacgaagc cggtgcccat gcccagcccg tcgagcgccg agcgggcgat
     3421 gccgttcttc gaggcgaacg cctcggcgcg tccgaggatc acacagttga ccacgatcag
     3481 cgagatgaac gcgccgagcg ccttgtgcaa ctcgaggctg atcgcctgga tcgcgtaatc
     3541 gacgacggtc acgaagctcg cgatcaccag gatgaacgtc gcgatgcgca cctggcgcgg
     3601 gatcagccga cgcaaagacg agacgacgac gtttgacatg acgaggacga acagcgtcgc
     3661 caaccccatc gccagcgcgt tgcgggcggt attggaaacc gcgagcacgg gacacatgcc
     3721 cagcacttgg acgaagaccg ggttgtcacg ccacagaccc ttgaccagct cgtcggtcgc
     3781 gcggtcgtcg ggggtcgagg tcggcaagct cgtcatcgcg tcatccgtcc ccgcgcaacg
     3841 cggcaaggtc gcgctggatg atcggcaccc agtcggccgt gctgcggcgc agcatgtcgg
     3901 cgatcgccac cgacgagatc gtcgcgccgg tgatgccgtc gacctgccac tgccggtcct
     3961 tgcgcccgtg tttgaccgcc tcgatcgggt gggcgatcgc ggacccgtcc gcggtcagcc
     4021 gcgcgtcgag ccgtgcgaag ttggcgagga actcggggtc ggtctcgatc cgatcgccga
     4081 gccccggggt ctccttgctc tccaacacgc ggattccgat caccgcggcg tgctccgggg
     4141 aatagccgta gagcacgcgg atcacgtcct gatagcccat gccctgggtc tcgatcgcgg
     4201 cgccgacgag cagtcccgcg gcgtcgtagc cggcgtagat gcgcggcccg ccggcatccg
     4261 cctcgccgtc ggcaaccacc agccggttgg acccgtcgag tcgaaagggc cggctcgtcg
     4321 ccgcgcccgg caggacgtcg aagatcgccc gctcgagggc ggcggcacgg ttccgctcga
     4381 tcaccggcct cgtcagctcg aagacggtga cgatcgacag gccgcagatc actccgacgc
     4441 cgaccatcgc ccggtacatc ggccaggcgg cgagcggggg t
//
LOCUS       DASPGN010000098        15786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1305779, whole genome shotgun sequence.
ACCESSION   DASPGN010000098 DASPGN010000000
VERSION     DASPGN010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 15786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15786
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1305779"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2416
                     /locus_tag="VD788_02730"
     CDS             <1..2416
                     /locus_tag="VD788_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXV75205.1"
                     /translation="MAMSDSLALDLDPQTRQALDLDELLDWVAGYARTFHGAEEIRGV
                     VPTADGPALEVLARTVEELMRHLAGEGPVVPAGLPSAAGGLARLRHAGQPIPAQELRD
                     LAAVVAAAGEIGQRLERMEAASHPRLHALGEGAPDLRRAVRVVLDAIAPDGSIADEAT
                     PELRRIRRHRAETTARLTRRLEGHLRAPGAGAVIRDDFITQRNGRFVIPVRSDSPHAV
                     RGIVHATSSSGATQFVEPLETVELNNDLVRLAEAETREIERVLGEWSDALRARADAVL
                     ASIALLARVDALQARALFAAATGGRPAVLDRSATIRLVGFRHPLLERWLAQTGRRAVP
                     FDLTLDPADRALVLSGPNAGGKTVTLKAIGLAALMAQCGVPLPALEAKLPLFRQVRAD
                     IGDHQSITADLSTFSAHVGAAARFLAERAPPSLFLFDEIGSGTDPAEGAALARAELEQ
                     LSGPGVTVVATTHQSSLKAWAHTAAGAVSAAMEFDTVELRPTYRVLADTAGLSAGLDI
                     ATRVGLPAAVVERARELLGPERTRAEDFLNRLQQQSRELARRTEELERRARELDDREI
                     ELERRRERAAELERQRLRAEFERVAQRLERESTEQLARLADPRERGRAARKLERIREQ
                     AATALARAIAPPATAGGPDEPIGADELAPGIEVWISSLGRAGVVDRVDGKHARVLVGA
                     LPTTVDRSELRRTRGGSGGAPRAGKQPEGGRRPARAEPDEESASAELMLIGRTVDEAT
                     AELDRFLDRAARAGLVEVRVVHGHGTGRLRRGVRQWLAGHPHVREYRPGRPTEGGDGA
                     TVVRLR"
     gene            complement(2413..2862)
                     /locus_tag="VD788_02735"
     CDS             complement(2413..2862)
                     /locus_tag="VD788_02735"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXV75206.1"
                     /translation="MPYLVDGDNLLGSWPGRRRSDAERRALGVELQRLAHHWRRRITA
                     VFDGRAPTALSAGGGVRFAGPGQSADQVILALLRAESDPAGWVVVTSDRSLGDQCRYL
                     GARVERCDRFRQRMAARAEQEKPDRVDDLDEWLELFDDDREEPGGRD"
     gene            complement(2862..4295)
                     /locus_tag="VD788_02740"
     CDS             complement(2862..4295)
                     /locus_tag="VD788_02740"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXV75207.1"
                     /translation="MGLPDGVVRDPLRALADAARARGSGILSATRGKQKRLICLVDGK
                     LAFVATNVIEEQLEQFLVVKGTVKADRVQAIAREAKQAGQRTALMLLQRNVLGADQLY
                     AAAAEHCKRLFESTLEWPGAGFLFSGGRPRLLGELTVDLPCLALLYDHARRFPKTMAE
                     VRERIGPAETRPIRVEDGKTALDGVELDDSARGVLELCDGFLTAAEVVARVRGSAEAA
                     WRAIHALMTVGALRAVAKGELPKTTVSQTVTRDEMLARLARDESDHYAVLGLTQKAAA
                     DEIRDAYYYLARRYHPDRHRTGELRDLMGRIEAFFTKVTEAYNTLYDPELRAKYDEEI
                     AVAGSAGASVEPQQDTRYLAKQNFARGRLLSEKKQYHDALKFFENAIELDPSKAEYHL
                     ELGQVLSFNPRRRDDAERALRRAVEMNPGLTRAYGILGEMFERAERVEDAIEMFEELL
                     RWESRNTNAAAALDRLTSGRRKRPSRS"
     gene            complement(4295..5614)
                     /locus_tag="VD788_02745"
     CDS             complement(4295..5614)
                     /locus_tag="VD788_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75208.1"
                     /translation="MTRPTLAPRWAVITAWIVVALAPSTRADRLHLEGGGLIDVDRWW
                     IEGDTLVYDAAGGTMGIPRSIVARIETTNRERSARRSDPPPTAPPAGRPTLVLDASDD
                     AELRDLMRRGRQALEAREFESASAYFLRVVEDRPEIYAARVGYVVSEIEMQHHGLALS
                     VVLDGIAREPTRPELYALLGELRYRDERLEDALVALRQAFEIEPSDDLRERILKVERE
                     LEASRFYDFASSSHFNLRYDGEVDPSLADAMIDYLETQYWVITESLRHAPPRPITVQL
                     FPLQQFREVTRAPDWAGGLYDGKIRVPLGGLTRLTPDAQRVLVHELTHAVVHSKSRGN
                     APRWLHEGLAQRSEGRTLLRADRQAIAAELRAFPPERWATLEFSYPLALSMTEFLESL
                     AGFAGLVQLIDEMGQGAAQDEALQRVYGRDYDRLCLEWGRQLLEREG"
     gene            complement(5611..6162)
                     /locus_tag="VD788_02750"
     CDS             complement(5611..6162)
                     /locus_tag="VD788_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75209.1"
                     /translation="MPMSNKSKRAGAGGDWRHLAVSGALLVAVGGTLLLGAVLEVPHD
                     RTAAPRTIEAISPVLPADEPAEPIDDTVEPGMVVPFDGDADLVDRATDDERRLHASGN
                     GYTLQFGVNCETKNVRANLEALDRYQDFYLLTMLYQDRACFRLCWGFYDNEAQARSDR
                     RIPEQLLRMTDAPKVISVSEALP"
     gene            complement(6275..6961)
                     /gene="lepB"
                     /locus_tag="VD788_02755"
     CDS             complement(6275..6961)
                     /gene="lepB"
                     /locus_tag="VD788_02755"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXV75210.1"
                     /translation="MGTRVGYAETIAVPPAKSTLREYAETILICVVVFVFLRGFVFQH
                     SEIPSGSMEDTVLPGDYILVNRFSYAPVSFELERRLLPTRRVERGDVVVFRHPHEPER
                     DFIKRVIGMPGETVELRRGFVFVDGRPLDEPYLNPLYREPEDFPPLEVPPSEYFLLGD
                     HRNDSADSRRWGTVPRRLIKGRAVLVLFSTEGPPPGADAGKVTIPSTLRKLRDIVLRS
                     RWDRAATLIR"
     gene            complement(6984..7685)
                     /gene="lepB"
                     /locus_tag="VD788_02760"
     CDS             complement(6984..7685)
                     /gene="lepB"
                     /locus_tag="VD788_02760"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXV75211.1"
                     /translation="MNGTAEAAAAEIVDRARSEPKSVAREYAETILICVIFVIFSRAF
                     VFQQSKIPSGSMMDTLLIGDYIMVNRFVYAGSSFDWERKLLPVRDLRRGDVVVFRMPR
                     EPEVDYIKRLIGLPGDVVEVRQGRVYVNGELLDEPYVKDDYRRGVSDMAAERVRADHY
                     FVMGDHRNASYDSREWGQVPRRLIKGRALLIWYSFPEEHAQSTEITRRMKSWVEKIVY
                     FPFRSRWSRCFTLIR"
     gene            complement(7756..9480)
                     /gene="ggt"
                     /locus_tag="VD788_02765"
     CDS             complement(7756..9480)
                     /gene="ggt"
                     /locus_tag="VD788_02765"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXV75212.1"
                     /translation="MHPSKPRLPRYARLLLVAAVVAGWLPLEAASRPAARGTDGMVVS
                     PEARATAVGVEVLAAGGNAVDAAVAVAFALGVTYPLAAGLGGGGFLLIRQPDGSYHAL
                     DFRETAPAAIGRELFVDDDGRPIAGLSLDTGLAVGVPGTVAGLAAAHARWGSLPWADL
                     VRPAISLARDGFEVYPWLEHAFDTEAEHLLADDAAREIFAPVGRPLASGQRLVQADLA
                     TTLEAVAQRGPRGFYEGQVAARLVAAVQKAGGVLNERDLADYEPSERRPIEGRYRGHR
                     VVTFPPPSSGGVTLLQMLAMLERFDLGSAGWGASLTVHLMTEAERRAFADRARWLGDP
                     GFYAVPLDRLLDTDYLKTRAASIRPDRATASRSVGPGDPSDDDRGETMHFSIGDGDGR
                     AIATTITLNRWFGTGIVAAGTGVLLNNEIDDFAIVPGTPNLYGLSGGEANAIAGGKRP
                     LSSMTPTIVEAPGGGPRPLLVLGSPGGSTIPTTILQVLLNVIDHGMTLQEAVDAPRLH
                     HQWQPDRIVHEPRALPPDVMVNLIARGHALVPAERPLGNVSAIGVAADGAWLGAADPR
                     RQGAAAGR"
     gene            9581..10108
                     /locus_tag="VD788_02770"
     CDS             9581..10108
                     /locus_tag="VD788_02770"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXV75213.1"
                     /translation="MKSLPLLALSMSLVVAACGGGGGASATAAPSAGAGAEQTLAPEF
                     ELPALTGDTIQLSDSAGRVRLIDFWATWCPPCRDEIPMLNELHRTYQDAGLTILAISD
                     ESTDVVREFAEEIGMDYTNLIDPGEVSQAYRVLGLPTAFLIDQEGRIVQSYMGPKPRK
                     MLEQKIRELLRLPPG"
     gene            10115..10576
                     /locus_tag="VD788_02775"
     CDS             10115..10576
                     /locus_tag="VD788_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75214.1"
                     /translation="MTRATRSALMLGLISLGGVVALAMIAERYGKVVRARQVGRDGRP
                     SAVEALPDAEATHYVARLLEVRRTVYRAYATRNEAASPEDLRPALDRAFEQALLDGGL
                     DRITYQEIDSVYRAWRQGSDEVPAAYRVELERQAAALAAVEIDGYDPLGLP"
     gene            complement(10562..10720)
                     /locus_tag="VD788_02780"
     CDS             complement(10562..10720)
                     /locus_tag="VD788_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HXV75215.1"
                     /translation="MKSYDFLFWAYNVIWLIIAGYLLSMVMRMKRLDERLGRLERELE
                     RDLGSGQT"
     gene            complement(10731..11423)
                     /locus_tag="VD788_02785"
     CDS             complement(10731..11423)
                     /locus_tag="VD788_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HXV75216.1"
                     /translation="MQRGAFDRAVLALWAVTTASMAVAIYLVFVYSPEERVMGAAQKI
                     FYFHVPIAMVTFLSVFVLLAGSVGYLWTRRLGWDHLSRAATETGLLFCSLVLLTGPIW
                     ARPAWGVWWTWEARLTTTLLLWLLLVAGLMVRGYADSRELGARLAAIIGIVAAVDVPI
                     IYKAVDWWRGQHPVLFKPGAKQPLAPEMATTFFFCIAVFLLLFVLLLAVRFRLAALEE
                     RTTRVVERATAA"
     gene            complement(11429..12127)
                     /locus_tag="VD788_02790"
     CDS             complement(11429..12127)
                     /locus_tag="VD788_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HXV75217.1"
                     /translation="MTRVGYFRATWHVARKDLQLEWRTLETLSSSFVFSLIVLVIFNF
                     AFGFETIKQIGAARLVPGVVWTVLAFGAVVSVARSMEIERRRDTLSALFIAPIDRGAL
                     FTGKFLANLVKNLLLQWIVVVLAAVFFNFDLVSIAGPMLLVLFVHGLGLTELGTLFAA
                     VASRVGRGEALLATLLFPATAPLFISAVKCTTSLLEGNGLSGAAASWLSISTGFDVLY
                     FLVALATFEFVLEE"
     gene            complement(12124..12873)
                     /locus_tag="VD788_02795"
     CDS             complement(12124..12873)
                     /locus_tag="VD788_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV75218.1"
                     /translation="MSPDEHPAPAIRTENLSKHYGAIHALNRMNLSFEAGESVALFGP
                     NGAGKTTLIRILTLSLRPSSGSFRIAGLDPRRDDLAIRRQIGVISHQSYLYDDLTARQ
                     NLEFFAGLYGVDDPRRRTAALLDSVGLAARADDPVRTFSRGMQQRASLARALVHEPQI
                     VFLDEPFTGLDPHGAMTLRDTLERLRQQRRTIVMVTHNLARGLELADRWLILARGRVV
                     DEGRSGEVDRAAFERSYFERLTSTARVREPA"
     gene            complement(12870..13223)
                     /locus_tag="VD788_02800"
     CDS             complement(12870..13223)
                     /locus_tag="VD788_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75219.1"
                     /translation="MSAGGIAAVIVGLSVVAWVLAPLLRKDAAESERIGSAVSEERDL
                     RSRREMLVAALKDLEDDRSTGKVDDTDYARSRTALSAEAVEIMKQLDALEQARRTSQP
                     VALPTPRDEPSGPPA"
     gene            complement(13220..13711)
                     /locus_tag="VD788_02805"
     CDS             complement(13220..13711)
                     /locus_tag="VD788_02805"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HXV75220.1"
                     /translation="MREVRRRAAVVIFAASLWSVLAAAPAAAFDDAAFNEAAGTILCD
                     CGCHPQSVAACTCGRAAEMRDEIRTMVASGMTGRQVIDDFVARYGEKILIAPEATGFN
                     LLAWLGPLGLLVGGLAALVVVVRRLSRRGPAGHAADAPPAAPVAADEPYAARLRREVE
                     ELR"
     gene            complement(13704..>15786)
                     /locus_tag="VD788_02810"
     CDS             complement(13704..>15786)
                     /locus_tag="VD788_02810"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HXV75221.1"
                     /translation="ALAAACLVAGFVTDDFRLDYVYHYSNTAQALPYKIGALWGGQAG
                     SLLLWVLILAVMASIMVLTNRRKNRGLMPWAVAVVGGTITFFMILLNFIEPVFATSAM
                     RADGVGLNPQLKNYWMMIHPPSLYLGYVGFTIPFAFAVGALVSRRTGDIWFRTTRRWT
                     LFSWFFLGIGILLGSYWAYIELGWGGYWAWDPVENASLMPWLTGTAFLHSVMIQEKKG
                     MLKIWNVLLIILTFSLSIFGTFLTRSGIISSVHAFATSDIVLPFGIFLVLVFFAPLIV
                     LLFRLEHLKTENRFEAVLSRESSFLFNNMILVGIALTVLLLTTFPMLSELVTSRKVTM
                     GPPIFNMVNIPWALALLTLTGIGPLIAWRRASAQNLKRNFVLPVLVGVWVLIAVSIFA
                     LPAYVDALRGIGRAIGRVDVAGVFAELKAFYPALTFSIGAFVLTTVWIEFWRGTRARV
                     SHYGESTAAALGRLVWRNKRRYGGYTVHVGVVVIFVGIAGSAAYQIEHTQALRPGEHL
                     QVDSYLMRYDGYRLEAVDDHIGAVTHVSLFDQRSGKPLGSIRAEQRFHPRLELDPLRE
                     AFLRVKQLGRDGAAGYQQAVAELFPLMSSLEQQVQREVKTPSTEVGIRSFRSPLDGAR
                     FAEDFYVTPLFVDPLSGQANFRIFVNPMVNLIWLGGLICIFGAHLSVLPDSRERRRLI
                     AAMELEDRAVA"
BASE COUNT         2591 a         5457 c         5519 g         2219 t
ORIGIN      
        1 catggccatg agcgacagcc tcgccctcga tctcgacccc cagacccgcc aggccctcga
       61 tctcgacgag ctcctcgact gggtcgcggg ttacgcgcgg accttccatg gtgccgagga
      121 gatccgcggc gtggtgccga cggccgacgg tccggcactc gaggtcttgg cgcggacggt
      181 cgaggagctg atgcgccacc tggcggggga gggtccggtc gttcccgccg ggctgccgtc
      241 ggccgcgggc ggactggcac ggctgcggca cgcgggtcag ccgatccccg cgcaggagct
      301 gcgggatctc gccgccgtcg tcgcggccgc gggagagatc ggccagcggc tcgagcggat
      361 ggaggcagcg tcccatccac gattgcatgc gctgggggaa ggcgcccccg atctgcgccg
      421 tgcggtgcgg gtcgtgctcg atgcgatcgc ccccgacggc tcgatcgcgg acgaggcgac
      481 acccgagctg cgacgcattc gccgccatcg cgccgagacg accgcgcgcc tgacgaggcg
      541 actcgagggg cacctgcgcg cgcccggtgc gggtgcggtg atccgcgacg atttcatcac
      601 ccagcgcaac gggagattcg tgatcccggt gcgcagcgac tcgccgcacg cggtgcgggg
      661 gatcgtgcac gcgacgtcgt cgtccggcgc gacccagttc gtcgagccgc tcgagacggt
      721 cgagctcaac aacgacctgg ttcggctcgc ggaggccgag acgcgcgaga tcgagcgcgt
      781 actcggcgaa tggtccgacg cgctccgggc gagggcggac gcggttctcg cttcgatcgc
      841 cctgctcgcg cgtgtcgacg cactccaggc gcgggcgttg ttcgccgccg cgacgggtgg
      901 tcgtccggca gtgttggacc ggtccgcgac gatccggctg gtcggctttc gccaccccct
      961 gctcgaacgg tggctggcgc aaaccgggcg gcgtgccgtg ccgttcgacc tgacgctcga
     1021 tcccgccgac cgggcgctgg tgttgtcggg cccgaacgcc ggcggcaaga ccgtgacgct
     1081 gaaggcgatc gggctcgcgg cgctgatggc gcagtgcggc gtgccgctgc cggcgctcga
     1141 ggcgaagctg ccgctgtttc gccaggtacg cgccgatatc ggggaccacc aatcgatcac
     1201 cgccgatctg tcgaccttct ccgcccacgt cggcgccgcc gcgcgctttc tggccgaacg
     1261 agcgccgccg tcgttgttcc tgttcgacga gatcggcagc gggaccgacc cggccgaggg
     1321 cgcggcgctg gcccgcgccg agctcgagca gttgtccgga cccggcgtca cggtcgtcgc
     1381 cacgacacac cagagctcgc tcaaggcgtg ggcccacacc gccgcggggg cggtcagcgc
     1441 ggcgatggag ttcgacaccg tcgagttgcg accgacctac cgcgtgctgg cggacaccgc
     1501 gggtctgtcc gccggtctcg acatcgcgac gcgggtcggg ctacccgcgg cggtcgtcga
     1561 gcgcgccagg gagctgctgg gacccgaacg gacacgggcc gaagacttcc tcaaccgcct
     1621 ccagcagcag agccgcgagc tcgcacgacg aaccgaggag ctcgagcgac gcgcgcgcga
     1681 gctcgacgat cgcgagatcg agctcgagcg ccgccgggag cgtgcggcgg agctcgagcg
     1741 gcaacgactg cgggccgagt tcgaacgcgt ggcgcagcgt ctcgagcgcg aatcgaccga
     1801 gcagctcgcg cggctggccg acccgcgcga gcgcgggcgt gcggcacgca agctggaacg
     1861 aatccgggaa caggccgcga cggcgctggc gcgggcgatc gcgcccccgg cgaccgccgg
     1921 cggaccggac gagccgatcg gggccgacga gctggcgccg gggatcgagg tctggatctc
     1981 ctcgctcggg cgcgccggtg tcgtcgaccg tgtcgacggc aagcacgcgc gggttctggt
     2041 cggggcgctg ccgaccaccg tcgatcgctc ggagctgcgc cggacgcggg gcgggagcgg
     2101 cggcgccccg cgggccggca agcagccgga gggaggacgg aggccggccc gagcggagcc
     2161 ggacgaggag tcggcttccg cggagctgat gttgatcggc aggaccgtcg acgaggcgac
     2221 ggccgagctc gaccggttcc tcgaccgagc ggcccgcgcc gggctcgtcg aggtgcgcgt
     2281 cgtccacggg cacgggacgg gccggttgag gcggggcgta cggcagtggc tcgccggcca
     2341 cccgcacgtc cgcgaatacc ggccggggcg cccgacggag ggcggcgacg gcgccaccgt
     2401 cgtccgcctg cgctagtcgc ggccgccggg ctcctcccgg tcgtcgtcga acagctcgag
     2461 ccactcgtcg agatcgtcca cccggtccgg cttctcctgc tccgcgcgcg ccgccattcg
     2521 ttggcggaag cggtcacagc gctcgacgcg agcgccgaga tagcgacact gatcgccgag
     2581 cgagcgatcg ctggtgacga cgacccagcc cgcgggatcc gactctgcac gcaacagtgc
     2641 gaggatcacc tggtcggcgc tctgtccggg accggcgaag cgcacccctc cgcccgccga
     2701 cagcgcggtc ggcgcacggc cgtcgaatac cgccgtgatg cgccggcgcc agtgatgggc
     2761 caggcgttgg agctcgacgc cgagcgcacg ccgctcggca tcggagcgcc gccggccggg
     2821 ccagctgccc agcaggttgt cgccgtcgac cagatagggc atcagctgcg ggacggcctc
     2881 ttgcgtcggc cggacgtgag tcggtcgagc gcggccgcgg cattcgtgtt ccggctttcc
     2941 cagcgcagca gctcctcgaa catctcgatc gcgtcctcga cgcgctcggc ccgctcgaac
     3001 atctcgccca ggatcccgta cgcccgggtc agacccgggt tcatctcgac tgcccgccgc
     3061 agcgcgcgtt cggcatcgtc gcgccgccgc gggttgaagc tgagcacctg ccccagctcg
     3121 aggtggtact cggccttgga cgggtcgagc tcgatcgcgt tctcgaagaa cttgagcgcg
     3181 tcgtggtact gtttcttctc ggagagcagc cggccgcggg cgaagttctg cttcgccagg
     3241 taccgcgtgt cctgctgcgg ttccacactg gctcccgccg acccggcgac cgcgatctcc
     3301 tcgtcgtact tggcccgcag ctccgggtca tacagcgtgt tgtaggcctc ggtcaccttg
     3361 gtgaagaacg cctcgatcct gcccatcagg tcccgcagct cgccggtgcg gtggcgatcg
     3421 gggtgataac gccgcgcgag gtagtagtag gcgtcgcgaa tctcgtcggc agccgccttt
     3481 tgggtcagcc cgagcacggc gtagtgatcg ctctcgtcgc gcgccaggcg tgccagcatc
     3541 tcgtcgcggg tcaccgtctg cgagacggtc gttttcggca gctcaccctt ggccacggcg
     3601 cgcagtgcgc cgaccgtcat caacgcgtgg atcgcacgcc acgccgcctc cgccgatccg
     3661 cgaacccggg ccacgacctc ggcggcggtc aggaagccgt cgcagagctc gagcacgccg
     3721 cgcgcggagt cgtcgagctc gactccgtcg agcgccgtct tcccgtcctc cactcgaatc
     3781 ggccgcgttt ccgccggacc gatccgctcg cgcacctcgg ccatcgtctt ggggaagcgc
     3841 cgcgcgtggt cgtacagcag cgcgagacag gggagatcga cggtaagctc tcccagaagt
     3901 cgcgggcgac caccgctgaa caggaagccg gcgcccggcc actcgagcgt cgactcgaac
     3961 agccgcttgc aatgctctgc cgcggccgcg tagagctggt ccgcgcccag cacgttgcgc
     4021 tggagcagca tcagcgccgt tcgctgaccg gcctgcttgg cctcgcgggc gatcgcctgc
     4081 acccggtcgg ccttgaccgt ccccttgacc acgagaaact gctcgagctg ctcctcgatc
     4141 acattggtgg cgacgaaggc caacttgccg tcgaccaggc agatcagccg cttctgcttg
     4201 ccccgcgtgg cgctcaggat gccggagcca cgtgcccgtg cggcgtcggc caacgcgcgc
     4261 agcggatcgc ggacgacgcc gtcggggagg cccatcagcc ttctcgttcg agcagctgcc
     4321 gcccccactc gaggcagagg cggtcgtagt cgcggccgta gacccgttgc agcgcctcgt
     4381 cctgcgcggc gccttgaccc atctcgtcga tcagctgtac cagaccggcg aagccggcga
     4441 gcgactcgag gaactcggtc atcgacagcg cgagcgggta ggagaactcg agcgtcgccc
     4501 agcgctccgg cgggaacgcg cgcagctcgg cggcgatcgc ctgccggtcg gcccgcagca
     4561 gcgttcgacc ctcgctgcgc tgggccagac cctcgtgcag ccagcgcggg gcattgccgc
     4621 gggacttcga gtggaccacg gcgtgggtca gttcgtgaac cagcacccgc tgggcgtcgg
     4681 gcgtgaggcg cgtcagcccg cccagcggaa cacgaatctt gccgtcgtac agaccgccgg
     4741 cccaatccgg cgcgcgggtc acctcgcgaa actgctgcag cgggaagagc tgaaccgtga
     4801 tcggccgcgg cggcgcatgg cgcaagctct cggtgatcac ccagtactgc gtctcgaggt
     4861 agtcgatcat cgcatcggcc aggctcgggt cgacctcgcc gtcgtaacgc agattgaagt
     4921 gggacgacga ggcgaagtcg tagaaccggc tggcttcgag ctcgcgttcg accttgagga
     4981 tccgctcgcg caggtcgtcg ctcggctcga tctcgaacgc ctggcgcagg gcgaccagcg
     5041 cgtcctcgag ccgctcgtcg cggtagcgca gctcgccgag cagcgcgtac agctcgggtc
     5101 gcgtcggttc gcgggcgatg ccgtcgagca cgaccgacag ggcgaggccg tgatgctgca
     5161 tctcgatctc actgacgacg tagccgacgc gcgcggcata gatctccgga cggtcctcga
     5221 ccacgcgcag gaagtaggcc gacgccgact cgaactcgcg cgcttcgagc gcctgccggc
     5281 cgcgtcgcat caggtcgcgg agctcggcgt catcgctcgc gtcgagcacg agggtgggcc
     5341 ggccggcagg gggagccgtc ggcggcggat cggagcgcct ggcggatcgc tcacggttcg
     5401 tcgtctcgat ccgcgcgacg atcgagcgcg ggatgcccat cgtgccaccg gcggcgtcgt
     5461 acaccagcgt atccccctcg atccaccagc gatcgacgtc gatcaggcct ccgccctcga
     5521 gatggaggcg gtcggcgcgg gtcgacgggg cgagggcgac gacgatccac gcggtgatga
     5581 cggcccagcg gggggcgagg gtaggccgcg tcaaggcagc gcttccgaga ccgagatcac
     5641 cttcggcgcg tcggtcatgc gcagcagctg ttccgggatc cggcggtccg agcgtgcctg
     5701 ggcttcgttg tcgtaaaagc cccagcagag ccggaaacag gcgcgatcct ggtagagcat
     5761 cgtcagcagg tagaagtcct ggtaccgatc gagcgcctcg aggttggcgc ggacgttctt
     5821 ggtctcgcag ttgacgccga actgcagcgt gtagccgttg cccgacgcgt gcaaccgacg
     5881 ctcgtcgtcc gtcgcccggt cgaccaggtc ggcgtcgccg tcgaacggca cgaccatccc
     5941 gggctcgacg gtgtcgtcga tcggctcggc cggctcgtcc gccggcagga ccggcgagat
     6001 ggcctcgatc gtgcgcgggg cggccgtccg atcgtgcggc acctcgagca cggcgccgag
     6061 cagcaacgtg ccgccgaccg cgaccagcag ggcgccgctg accgcgaggt gtcgccaatc
     6121 cccgccggca ccggcccgct tgctcttgtt cgacatcggc accccttttc ctcgtctcaa
     6181 tatatggccg cggagcgcag ctgtcttccc gccgtcgcgc ccgggcatga tagatcgcac
     6241 cggccgcccg ttccaaagac ggccttacgc cggctcagcg gatcagcgtc gcagcgcggt
     6301 cccaacgcga gcggagcacg atgtcccgca gcttgcgcag cgtcgagggg atcgtcacct
     6361 tgccggcgtc ggcaccgggc ggcggacctt cggtcgagaa cagcacgagc accgcgcgac
     6421 ccttgatcaa ccggcgggga accgtgcccc agcgccggct gtcggccgag tcgttgcgat
     6481 ggtcgcccaa caggaagtac tcgctcgggg gtacttcgag cggcgggaag tcctccggtt
     6541 cacgatagag cggattgaga tacggctcgt ccagcggccg gccgtcgacg aagacgaagc
     6601 cgcgccggag ctcgaccgtc tctcccggca tgccgatcac gcgcttgatg aagtcccgtt
     6661 ccggttcgtg cggatgacgg aagacgacga cgtcgccccg ctccacgcgg cgcgtcggca
     6721 gcagccgtcg ttcgagctcg aacgagaccg gggcgtagct gaaccgattg accaggatgt
     6781 agtcgccggg gagcaccgtg tcctccatcg agcccgacgg gatctccgag tgctggaaga
     6841 cgaagccccg gagaaagacg aagaccacca cgcagatcag gatcgtctcg gcgtactcgc
     6901 gcagcgtgct cttggccggc ggcacggcga tcgtctccgc gtatccgacg cgtgtgccca
     6961 ctcggtcgtc tcccgcaagc cggtcagcgg atcagggtga agcagcgact ccagcgcgaa
     7021 cggaacggga aatagacgat cttctccacc caggacttca tccgtcgcgt gatctcggtc
     7081 gattgggcgt gctcctcggg aaacgagtac cagatcaaca gcgcccgccc cttgatcagg
     7141 cggcgcggga cctggcccca ctcgcggctg tcgtaggagg cgttgcgatg gtcgcccatg
     7201 acgaaatagt ggtccgcacg gacgcgctcg gcggccatgt cggaaacacc tcggcggtag
     7261 tcgtccttga cgtacggctc gtcgagcagc tcgccgttga cgtagacccg gccctggcgg
     7321 acctcgacca cgtcgcccgg caggccgatc aagcgcttga tgtagtcgac ctccggctcc
     7381 ctgggcatcc tgaacaccac gacgtcgccg cggcgcagat cgcgaaccgg caggagcttc
     7441 cgctcccaat cgaacgaact accggcgtag acgaaacgat tgaccatgat gtagtcgccg
     7501 atcagcagcg tatccatcat cgagccgctc gggatcttcg actgctggaa gacgaaggcg
     7561 cgcgagaaga tgacgaagat cacgcagatc aggatcgtct cggcgtactc gcgcgcgacc
     7621 gatttcggtt cggatcgggc gcggtcgacg atctcggcgg cggcggcctc ggcggttccg
     7681 ttcatcgttc tcccgtcact gcgttccggc tggaaaggga aagagtacca cgcgagcccg
     7741 tacgccggca ggcggtcagc gaccggcggc cgcgccctgc cgacgcgggt ccgccgcgcc
     7801 gagccacgct ccgtcggccg cgacgccgat cgcgctgacg ttgccgagcg gccgctccgc
     7861 cgggacgagc gcgtggccgc gcgcgatcag attgaccatc acgtcgggcg gcagcgcgcg
     7921 aggctcgtgg acgatgcggt ccggttgcca ctggtggtgc agcctgggtg cgtcgaccgc
     7981 ctcctgcagc gtcatcccgt ggtcgatgac gttgagcagc acctggagga tcgtcgtcgg
     8041 aatggtcgac cctccgggcg agccgaggac cagcagcgga cgaggccctc cgccgggcgc
     8101 ctcgacgatc gtcggcgtca tcgacgacag cgggcgcttt cctccggcga tcgcgttggc
     8161 ctcgccgccg ctcagtccgt agagattggg ggtcccgggg acgatcgcga aatcgtcgat
     8221 ctcgttgttg agcaacacac cggttcccgc cgcgacgata cccgtgccga accatcggtt
     8281 gagggtgatc gtcgtcgcga tcgccctgcc gtcgccatca ccgatggaga agtgcatcgt
     8341 ctcgccgcga tcatcgtcgc tcgggtcgcc cgggccgacc gagcgcgatg cggtcgcgcg
     8401 atcgggccga atcgacgcgg cgcgggtctt caggtagtcg gtgtcgagca agcggtcgag
     8461 cggaacggcg tagaagcccg ggtcgccgag ccagcgggcg cgatcggcga aggcgcgtcg
     8521 ctcggcctcg gtcatcaggt ggacggtcag cgacgccccc cagccggccg aaccgagatc
     8581 gaagcgctcg agcatcgcga gcatctgcag cagagtgact ccaccactgc tcggcggcgg
     8641 gaaggtcacc acgcggtggc cgcggtagcg accttcgatc ggccgacgct cgctcggctc
     8701 gtagtcggcc agatcgcgct cgttgagcac accgccggcc ttctgcaccg ccgcgaccag
     8761 gcgcgccgcg acctgtccct cgtagaaccc gcgcgggccg cgctgggcga cggcctcgag
     8821 cgtggtggcg aggtcggctt gcacgagccg ctggcccgag gcgaggggcc ggcccaccgg
     8881 cgcgaagatc tcgcgggcgg catcgtccgc gagcagatgc tcggcctcgg tatcgaacgc
     8941 gtgctcgagc cacggataga cctcgaagcc gtcgcgggcg agcgagatcg cgggacgcac
     9001 gagatcggcc cagggcaggc tgccccagcg cgcgtgggcc gccgcgaggc cggccaccgt
     9061 cccggggaca ccgacggcga gccccgtatc gaggctcagc ccggcgatcg gccgcccgtc
     9121 gtcgtcgacg aacagctcgc ggccgatggc cgccggtgcg gtctcgcgga agtcgagcgc
     9181 gtggtagctg ccgtcgggtt gtcggatcaa gagaaagccg cctccgccca gcccggcggc
     9241 caacggatag gtcacgccga gtgcgaacgc gacggcgacc gcggcgtcga cggcgtttcc
     9301 gcccgccgcc agcacctcga cgccgaccgc ggtggcgcgc gcttcggggc tgacgaccat
     9361 cccgtcggtg ccgcgggcag ccgggcggga cgcggcctcg agtggcaacc agccggcgac
     9421 gaccgccgcg acgagcaaca gccgcgcgta tcgtgggaga cgcggcttcg acgggtgcat
     9481 gcggtctctc ctgcgggcct cgacgaagct aacatgcggc gaaaagccgt gtcaaagata
     9541 gctgctatca tgaggggatg ccgccgagca ggagtcccgg atgaagagcc tgccgcttct
     9601 cgcgctgtcg atgtcgttgg tcgtggccgc ctgcggcggc gggggcgggg cttcggccac
     9661 cgccgcgccg tcggccggcg ccggcgcgga gcagacgctc gcgcccgagt tcgagctgcc
     9721 cgcgttgacg ggggatacga tccagctcag cgattccgcg gggcgcgtgc gtctgatcga
     9781 cttctgggcc acgtggtgcc cgccctgccg cgacgagatc ccgatgctca acgagctgca
     9841 ccggacctac caggacgcgg ggctgacgat tctcgccatc tccgacgaga gcaccgacgt
     9901 cgtccgcgag tttgccgagg agatcggcat ggactacacg aacctgatcg atccgggcga
     9961 ggtcagtcag gcctaccgcg tgttggggtt gcccaccgcg ttcctgatcg accaggaagg
    10021 gcggatcgtc caatcgtaca tgggtcccaa gccccgcaag atgctcgaac agaagatccg
    10081 cgagctgctg cgcctgccgc cgggttgagc gacgatgacg cgcgcgacgc gcagtgcgct
    10141 gatgctcggt ctgatctcgc tcggcggtgt ggtggcgctg gcaatgatcg ccgagcgcta
    10201 cggcaaggtg gtccgggccc ggcaggtcgg acgggacggt cggccgagcg cggtcgaggc
    10261 gctacccgac gccgaggcca cgcactacgt cgcgcggctg ctcgaggtcc ggcgaacggt
    10321 ctatcgagcc tacgccacgc gcaacgaagc ggcgagcccg gaagacctgc ggccggcgct
    10381 cgaccgtgcg ttcgagcaag cactgctcga cggcgggttg gatcggatca cctaccagga
    10441 gatcgacagc gtctatcgcg cgtggcggca ggggagcgac gaggtaccgg cggcctaccg
    10501 cgtcgaactc gagcgtcaag ccgcggcgtt ggccgcggtc gagatcgacg gctacgatcc
    10561 gctaggtctg ccctgagccg aggtcacgct ccagctcgcg ttcgagccgc ccgagccgct
    10621 cgtcgagccg cttcatccgc atcaccatcg acagcaagta cccggcgatg atcagccaga
    10681 tcacgttgta ggcccagaac aggaagtcgt agctcttcac cggatcctcc tcacgcggcg
    10741 gtggcgcgct caaccactct cgtcgttcgc tcttcgagcg ccgccagccg aaaccgcacg
    10801 gcgagcaaca gcacgaacag caacaggaag acggcgatgc agaagaagaa ggtcgtcgcc
    10861 atctcgggcg ccagcggctg cttcgcgccc ggtttgaaca ggaccgggtg ctgtccgcgc
    10921 caccaatcga ccgctttgta gatgatcggc acgtcgaccg ctgcgacgat cccgatgatc
    10981 gccgccaggc gggcgcccag ctcgcgactg tcggcgtagc cgcggaccat caggccggcc
    11041 accagcagca gccacagcag cagcgtggtc gtcagccgag cctcccaggt ccaccagacg
    11101 ccccaggccg gtcgggccca gatcggtccg gtcaacagca ccaacgagca gaacaacagc
    11161 ccggtctcgg tggccgcacg ggacaggtgg tcccagccga gccggcgcgt ccacaggtac
    11221 ccgaccgaac cggccagcag gacgaacacc gacaggaagg tcaccatcgc gatcgggacg
    11281 tgaaagtaga aaatcttctg ggccgccccc atcacgcgct cctccggcga gtagacgaag
    11341 accagataga tcgcgacggc catggacgcc gtggtcacgg cccacagggc gagaaccgct
    11401 cggtcgaacg ctccgcgctg catcgaactc actcctcgag aacgaactcg aacgtggcca
    11461 gggccacgag aaagtaaagc acgtcgaacc ccgtcgagat cgaaagccag ctcgccgccg
    11521 caccgctcag tccgttgccc tcgagcaggg aggtcgtgca cttgaccgcc gagatgaaca
    11581 gcggtgcggt ggccgggaac agcaacgtgg ccaacagcgc ctcgccgcgc ccgacccggc
    11641 tggcgacggc ggcgaacagc gtgccgagct cggtcaaccc caacccgtgg acgaacaaca
    11701 ccaagagcat cggtccggcg atcgagacca ggtcgaaatt gaagaagacc gcggcgagca
    11761 cgacgacgat ccactgcagc agcaggttct tgaccaggtt ggcgaggaac ttgcccgtga
    11821 acagcgcgcc gcggtcgatc ggcgcgatga acaacgccga cagcgtgtcc cgccggcgct
    11881 cgatctccat cgaccgcgcg acgctgacca ccgcaccgaa cgccaacacg gtccagacca
    11941 cgcccggaac gagccgggcc gcgccgatct gtttgatcgt ctcgaaaccg aaggcgaaat
    12001 tgaatatgac cagcacgatc agcgagaaaa cgaagctcga cgacagggtc tcgagcgtgc
    12061 gccactcgag ctggagatcc ttgcgggcga cgtgccaggt ggcgcggaag tagccgacac
    12121 gggtcatgcc ggctcccgca cgcgcgcggt gctggtcagg cgttcgaagt agctccgttc
    12181 gaacgcggcg cgatcgacct cgcccgaacg accctcgtcg accacgcggc cgcgggccag
    12241 gatcagccag cggtccgcca gctcgagccc gcgagccagg ttgtgcgtca ccatcacgat
    12301 cgttcttcgt tgttggcgca gccgctcgag cgtgtcgcgc aaagtcatcg ccccgtgtgg
    12361 atcgaggccg gtgaacggct cgtcgaggaa gacgatctgc ggctcgtgga cgagcgcgcg
    12421 ggccagcgag gcccgctgct gcatgccacg cgagaaggtg cggaccgggt catcggcgcg
    12481 ggcggcgagc ccgacgctgt cgagcaacgc cgcggtgcgc cgacgcgggt cgtcgacgcc
    12541 gtacagcccg gcgaagaact cgagattctg ccgagcggtc aagtcgtcgt agaggtagga
    12601 ctgatgggag atcacgccga tctgccggcg aatggccagg tcgtcccggc gcgggtcgag
    12661 accggcgatg cggaacgagc cgctgctcgg ccgcaggctc agcgtcagaa tgcggatcaa
    12721 tgtcgtcttt ccggcgccgt tgggaccgaa cagcgcgacc gactcgccgg cttcgaacga
    12781 caggttcatc cggttcagcg cgtggatcgc cccgtagtgc ttcgacaggt tttcggtgcg
    12841 gatcgccggg gccgggtgtt cgtcggggct cacgcgggcg gcccgctcgg ctcgtcacgc
    12901 ggggtcggca gcgcgaccgg ctgagaggtc ctacgcgcct gctcgagggc gtcgagctgc
    12961 ttcatgatct cgacggcctc cgccgaaagt gcggttctcg atcgggcata gtccgtatcg
    13021 tcgactttcc ccgtcgaccg gtcatcctcg aggtccttga gcgcggccac cagcatctcc
    13081 cggcgggagc gcagatcccg ctcctcgctg accgccgagc cgatccgctc ggactcggcg
    13141 gcgtccttgc gcagcagcgg ggcgaggacc caggccacga cggacagccc gacgatcacg
    13201 gcagcgatgc cgcccgcgct catcgcagct cctcgacctc gcggcgcagc cgggccgcat
    13261 acggctcgtc ggccgcgacc ggcgcggcag gcggggcgtc ggccgcgtgg cccgccggcc
    13321 cgcgccggct cagccgacgg acgacgacga cgagcgccgc gaggccgccg accagcagcc
    13381 cgagcggtcc gagccaagcg agcagattga agccggtggc ctcgggtgcg atcaggatct
    13441 tctcgccgta gcgcgcgacg aagtcgtcga tcacctgccg ccccgtcatc ccggaggcga
    13501 ccatagtccg gatctcgtcg cgcatctcgg cggcgcgccc gcaggtacac gcggcgaccg
    13561 actgggggtg gcagccgcag tcgcagagaa tcgtcccggc cgcctcgttg aacgcggcgt
    13621 cgtcgaacgc cgcggcgggg gcggccgcga gcacggacca gagcgaggcg gcaaagatga
    13681 cgacggccgc cctccgccgt acctcacgca acggcgcgat cctccagctc cattgccgcg
    13741 atcaggcgcc ggcgctcccg cgagtcggga agcacggaca ggtgcgcgcc gaagatgcag
    13801 atcagcccgc cgagccagat caggttgacc atcgggttga cgaagatacg gaagttggcc
    13861 tgcccgctga gcggatcgac gaacagcggc gtgacgtaga aatcctcggc gaagcgcgcg
    13921 ccgtcgagcg gggagcgaaa cgagcggatg ccgacctccg tcgacggggt cttgacctcg
    13981 cgctgcacct gctgctcgag cgagctcatc agcgggaaca gctcggcgac ggcctgctgg
    14041 tatcccgccg ccccatcccg gcccaactgc ttgacccgca gaaaagcctc gcgcagcgga
    14101 tcgagctcga gccgcggatg gaaccgctgc tcggcacgga tgctacccaa cggcttgccg
    14161 ctgcgctggt cgaacagcga cacgtgcgtg accgcgccga tgtgatcgtc gacggcttcg
    14221 aggcgatagc cgtcgtagcg catcaggtac gaatcgacct gcaggtgctc gccgggccgc
    14281 aacgcctgcg tgtgctcgat ctggtaggct gcggatccgg cgatgccgac gaaaatcacg
    14341 acgacgccga cgtggacggt gtagccgccg tagcgacgct tgttgcgcca gaccaggcgc
    14401 ccgagcgcgg cggcggtcga ctcgccgtag tggctgaccc gggcgcgcgt gccccgccag
    14461 aactcgatcc agacggtcgt cagcacgaac gcgccgatcg agaaggtcag cgccggatag
    14521 aacgccttca gctcggcgaa cacaccggcc acgtcgactc ggccgatcgc gcggccgatc
    14581 ccgcgcagcg catcgacgta ggccggcagc gcgaagatcg agacggcgat cagcacccac
    14641 accccgacga gcaccggtag cacgaagttg cgtttgagat tctgcgccga ggcgcgccgc
    14701 caggcgatca acggaccgat cccggtcagc gtcaacaacg ccaacgccca cgggatgttc
    14761 accatgttga agatcggcgg gcccatcgtc accttgcgcg aggtgaccag ctcggacagc
    14821 atcgggaagg tcgtcagcaa cagcacggtg agcgcgatgc cgacgaggat catgttgttg
    14881 aacaggaagg acgattcgcg ggagaggacg gcctcgaacc ggttctcggt cttgaggtgc
    14941 tccagccgga acaacagcac gatcaacggt gcgaaaaaga ccagcacgag aaagatgccg
    15001 aagggcagca cgatgtcgga cgtcgcgaac gcgtggaccg acgaaatgat gccgctccgc
    15061 gtcagaaagg tgccgaagat cgacaacgag aaggtcagga tgatcagcag gacgttccag
    15121 atcttgagca tccccttctt ttcctggatc atcaccgagt gcaagaaggc ggtgccggtc
    15181 aaccacggca tcagcgaggc gttctccacc gggtcccagg cccagtagcc accccagccc
    15241 aactcgatgt aggcccagta cgacccgagc aggatgccga tgccgaggaa gaaccacgag
    15301 aacaacgtcc agcgtctcgt cgtccggaac cagatgtcgc cggtgcggcg gctgacgagc
    15361 gccccgaccg cgaaggcgaa cggaatggtg aagccgacgt agcccaggta gagcgacggc
    15421 gggtggatca tcatccagta gttcttgagc tggggattga ggccgacgcc gtcagcgcgc
    15481 atcgcactgg tcgcgaagac cggctcgatg aagttcagca ggatcatgaa gaacgtgatc
    15541 gtgccaccga cgacggcgac ggcccacggc atcagcccgc ggttcttgcg ccggttggtc
    15601 agcaccatga tgctggccat caccgccagg atcagcaccc acagcaacag gctgccggcc
    15661 tgcccgcccc acagcgcgcc gatcttgtac ggcagcgcct gcgcggtgtt cgagtagtgg
    15721 tagacgtagt ccaggcggaa gtcgtcggtg acgaagcccg cgaccaggca ggcggcggcg
    15781 agagcg
//
LOCUS       DASPGN010000099         3787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_253007, whole genome shotgun sequence.
ACCESSION   DASPGN010000099 DASPGN010000000
VERSION     DASPGN010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253007"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VD788_02815"
     CDS             complement(<1..718)
                     /locus_tag="VD788_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HXV75222.1"
                     /translation="MMRRLFDHVHLWRPKHWVKNVFVLMPVPFGLAAGARADAGQFLL
                     GFVGFCFATSAVYALNDVIDAEQDRRHPRKQERPVASGRLRPVEGLASAAVALGVALG
                     LSWTVHSTTAITLVGVYVGLNVTYSLGAKHVPLIDVFILSSGFVLRVLLGCALIDVRP
                     SSWLLLCSSALALFLALAKRRGDLVRGVGPDHRPSLRGYNVEFLNQAITISTALTLMS
                     YALYTMEAEVLVPGREFASLP"
     gene            complement(724..1464)
                     /locus_tag="VD788_02820"
     CDS             complement(724..1464)
                     /locus_tag="VD788_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV75223.1"
                     /translation="MLKVVAFGATRGMGRALARLMAERGDRLFLLGRDENSLARSARD
                     LAHSTGAGELGYATCDLDRRSSFEPALERADAALGGFDTVVVSAATFETQERLENDLS
                     ATERLLDADFTKTVVFCECVRLRLLARGGGTLCVFSSVAGDRARKPVVLYGAAKAGLS
                     YYLEGLDHRYHHAGLRVVCVKPGFVKTGMTAALDPPPFAGEPDVVARTVLRAIDAARP
                     VVYAPAAWRGIMTLIRWLPRSVMRRVEF"
     gene            complement(1458..2771)
                     /locus_tag="VD788_02825"
     CDS             complement(1458..2771)
                     /locus_tag="VD788_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXV75224.1"
                     /translation="MRAISGWGRYPVVLADERVDEDLARITLGASLTRGLGRSYGDAS
                     LPAVAGAVVAGSRLADRIIEFDRRRGLLRAEAGLSLSRLHRVAWPVGWSSPVIPGTQF
                     VSLGGMVAADVHGKNHHVRGSFGNHVRALRMRLADGRIVEIDEGSEPELFRATLGGMG
                     LTGHLLEVELALEPIPSPWIHQQKERFADLDSLLVGLGRAGDAWPFTVAWADCLARGS
                     SMGGGILIKGRWARKDEAPRAIPGAGPRVELPFDLPSWVLAEPAVRLFNTAYLHWLGM
                     RHAGDVVDPVGFFHPLDAVTGWNRLYGRHGFIQYQCVVADRAAVHRLFDVLARRAAPV
                     FLAVIKDFGPTSRGMISFPMPGLTVSLDLPFRGDATQKLVDELNEVVVDGEGRVYLAK
                     DALTRDEHFRAMEPRLARWTAVRRAWDPARRIKTALSARLLGDAC"
     gene            2971..>3787
                     /locus_tag="VD788_02830"
     CDS             2971..>3787
                     /locus_tag="VD788_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB domain-containing protein"
                     /protein_id="HXV75225.1"
                     /translation="MRFVRAGGREIVLVGTAHVSRESAELVRRVIEEERPDRVCIELD
                     EQRYRALTQKTNWEKLDLKQLIRTKQLTTLLANLLLASYQKRLGLKLGVMPGTELLEA
                     DATARELGIPVSLCDRNVRVTVLRAWRSMSWWKKNMLLAGLLAGMFGEHDLSEDDLRE
                     LRRRDVLSELINELAREMPVLKRVLIDERDHYLAQKIRAGEGGRIVAVVGAGHLDGVE
                     AALMEEGGEDLGPLETIPEVFPGWKIAGWMIPVAIVSGLAAVAILEGPEVAGRN"
BASE COUNT          684 a         1281 c         1311 g          511 t
ORIGIN      
        1 acggaagcga ggcgaactcg cgtccaggta cgagcacctc cgcctccatc gtgtagagcg
       61 cgtacgacat cagcgtcagc gcggtggaga tggtgatcgc ctggttcagg aactcgacgt
      121 tgtagccgcg caggctgggc cgatggtcgg ggccgacgcc gcgcaccagg tcgccgcgtc
      181 gtttggccag cgcgaggaac agcgcgagcg ccgaggagca gagcagcaac cagctcgacg
      241 ggcgaacatc gatcaacgca cagccgagca agacccgcaa cacgaatccc gaggacaaga
      301 tgaacacgtc gatcaacggc acgtgcttgg cgccgagact gtaggtcacg ttcagaccga
      361 cgtagacgcc gaccaaggtg atggcggtcg tgctgtggac ggtccacgac aggccgagcg
      421 cgacgccgag cgcgacggcg gccgaggcga gcccctcgac cgggcggagt cggccggacg
      481 cgaccggccg ctcctgcttg cgggggtggc ggcggtcctg ctcggcgtcg atcacgtcgt
      541 tcagcgcgta gaccgcggag gtggcgaaac agaagccgac gaacccgagc aggaactggc
      601 cggcgtcggc gcgtgcgccg gccgcgagcc cgaacggcac cggcatcagc acgaacacgt
      661 tcttgaccca gtgtttcggt cgccacaggt ggacgtgatc gaacagcctg cgcatcatcg
      721 gtgtcagaat tccacgcgac gcatgacgga acgtggcaac caacgaatca gcgtcatgat
      781 cccgcgccac gcggcggggg catagacgac cgggcgggcg gcatcgatcg cgcgcagcac
      841 cgtccgcgcg acgacgtcgg gctcgccggc gaacggcggc ggatcgagcg cggccgtcat
      901 cccggtcttg acgaagcccg gcttgacgca gaccacgcgt agcccggcat gatggtagcg
      961 gtggtcgagg ccctcgaggt agtacgacaa cccggctttc gccgccccgt agaggacgac
     1021 cggcttccgc gcacgatcac cggcgaccga cgagaagacg cacagcgtgc cgcccccgcg
     1081 cgcgagcagc cgcagtcgga cgcactcgca gaacaccacg gtcttggtga agtcggcgtc
     1141 gagcagccgc tcggtggcgg agagatcgtt ctcgagccgc tcctgggttt cgaaagtcgc
     1201 cgcgctgacg acgaccgtgt cgaaaccgcc gagcgcggcg tcggcccgct ccagcgccgg
     1261 ctcgaagctc gatcgtcggt cgagatcgca ggtggcgtag ccgagctcgc cggcgccggt
     1321 gctgtgggcg agatcgcgcg cgctgcgggc gagggagttt tcgtcgcgcc cgagcaggaa
     1381 caggcggtcg ccccgctcgg ccatcagtcg tgcgagcgcg cggcccatcc cgcgcgtagc
     1441 gccgaaggcg acaaccttca gcacgcatcc cccagcagtc gcgcagacag ggcggtcttg
     1501 atgcgccgtg ccggatccca cgcgcggcgc acggcggtcc accgcgcaag cctcggttcc
     1561 atcgcccgaa agtgctcgtc ccgggtcagt gcgtccttgg ccaaatagac ccggccttcg
     1621 ccgtcgacca cgacttcatt cagttcgtcc accagcttct gcgtcgcgtc cccacggaac
     1681 ggcaggtcga gagaaacggt cagccccggc atcgggaacg agatcatacc ccgactcgtc
     1741 gggccgaagt ccttgatcac ggcgaggaag accggggccg cgcggcgggc cagcacgtcg
     1801 aacaagcggt gtacggcggc ccgatcggcg acgacgcact gatactggat gaacccgtgc
     1861 cggccgtaga gccggttcca accggtgacc gcgtccagcg gatggaagaa gccgaccgga
     1921 tcgacgacgt cgccggcgtg acgcataccg agccagtgca ggtaggcggt gttgaacagc
     1981 cgtaccgccg gttccgccag cacccacgac ggcagatcga acggcagctc gacgcgcggg
     2041 ccggcgccgg ggattgcgcg aggagcttcg tccttgcgtg cccaacgacc cttgatcagg
     2101 atgccgccgc ccatcgagct cccgcgagcg aggcaatcgg cccaggcgac ggtgaacggc
     2161 cacgcgtcgc ccgcgcgtcc taagccgacc aacaacgaat cgaggtcggc gaagcgctcc
     2221 ttctgctggt ggatccacgg ggatggaatc ggttcgagcg cgagctcgac ctcgagcagg
     2281 tgcccggtca accccatgcc gccgagggtc gcgcggaaca gctccggctc cgacccttcg
     2341 tcgatctcga cgatccgtcc gtccgcgagc cgcatgcgca acgcgcggac gtggttgccg
     2401 aacgacccac ggacgtggtg gttcttgccg tggacgtcag ccgcgaccat gccgcccaac
     2461 gagacgaact gcgttcccgg aatgaccggg ctcgaccaac ctaccggcca agcgacccgg
     2521 tgcaggcgcg aaagcgacaa cccggcctcg gcacgcagca gaccgcgccg gcgatcgaac
     2581 tcgatgatgc gatcggccaa gcggctgccg gcgacgacgg ctccggccac ggccggcaaa
     2641 gaggcgtcgc cgtaggaccg cccgagcccc cgcgtcaacg acgccccgag ggtgatccgg
     2701 gcgagatcct cgtcgacgcg ctcgtcggcc agcacgacgg ggtagcgccc ccagcccgaa
     2761 atcgcccgca tccgctccac ccgcaagcac ccggcggcgc ccgtcgagac ttatcgcggc
     2821 ggaatgatag gtcgagcgac ccgcgtgaat ccaccgtatg cgccggcgtc ggaaaacggt
     2881 cataatcccg cccggtccgc ggctcttcga catcgggaga aacaagccat cgaaagcgag
     2941 gctcgatcgt catgcggtta tccgggcgag gtgcggttcg ttcgggccgg cggccgggaa
     3001 atcgtcctgg tcggcacggc gcacgtttcg cgtgagtccg ccgagctggt ccgccgcgtg
     3061 atcgaagagg aacggccgga ccgtgtctgc atcgagctcg acgaacaacg ctaccgcgca
     3121 ctgacccaaa agacgaactg ggagaagctg gacctcaagc agctgattcg aaccaagcag
     3181 ctgaccacct tgctggccaa tctgctgctc gcctcctacc agaagcgtct ggggctgaaa
     3241 ctcggcgtga tgccgggtac cgagctgctc gaggcggacg ccaccgctcg cgagctcggc
     3301 atcccggtct cgctgtgcga tcgcaacgtg cgcgtgacgg tcctgcgtgc ctggcgctcg
     3361 atgtcgtggt ggaagaagaa catgctgttg gccgggttgc tggccggcat gttcggcgag
     3421 cacgatttgt ccgaggacga cctgcgcgag ctgcgccgac gcgacgtgct gagcgagctc
     3481 atcaacgaac tggcgcgcga gatgccggtg ctgaagcgcg tgctgatcga cgaacgagat
     3541 cactacctcg cgcagaagat ccgcgccggt gaaggcggac gcatcgtcgc cgtcgttggc
     3601 gccgggcacc tcgacggtgt cgaagcggcg ctgatggaag agggcggcga ggacctcgga
     3661 ccgctcgaga cgattcccga ggtgttcccc ggatggaaga tcgccggctg gatgatcccg
     3721 gtcgcgatcg tgtcggggct ggcggcggtg gcgatcctcg agggccccga ggtcgcgggt
     3781 cgcaacc
//
LOCUS       DASPGN010000100         9041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_17962, whole genome shotgun sequence.
ACCESSION   DASPGN010000100 DASPGN010000000
VERSION     DASPGN010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9041
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17962"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VD788_02835"
     CDS             complement(<1..301)
                     /locus_tag="VD788_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXV75226.1"
                     /translation="MTDPTRVYLDHNATSPLRPEAKEVMLAALAGPQANPSSLHAEGR
                     ARRALIEDARAEVAELVGARASEVVFTSGGSEALAAAIRGVCDRAPDGLRRIVVAE"
     gene            386..3379
                     /gene="ileS"
                     /locus_tag="VD788_02840"
     CDS             386..3379
                     /gene="ileS"
                     /locus_tag="VD788_02840"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HXV75227.1"
                     /translation="MSKNVELKSTLNLPSTRFSMKANLPQKEPEILTWWDSIDLFDRI
                     RRARAGDAPYVLHDGPPYANGKIHLGQAMNKILKDFVVKSHTMSGRNAVYVPGWDCHG
                     LPIEHRVDKELGPRKARMTPLEIRARCRAYAEKFVAVQGDEFRRLGVVWDRRSELAER
                     AEDRATRNSIYRTIDHAYEAEIIRQLAGFFTRGAIYHGVKPVHWCWSCRTALAEAEVE
                     YADRTDPSIYVKFPVDGLDRRVPALAGRDVAVVIWTTTPWTLPANMAVALHPELTYVA
                     IEVGRETLIVAEGLLGSLGATLGWDAPREVARFTGRELVGTGDDWVGHDAPVRRPYST
                     PTEPAGGGGVLILGEHVTLDAGTGCVHTAPGHGAEDFHVGKQYGLDVFNPVDDEGRFI
                     PSKVTEEWLAGAFVLDANRSIVEDLDRRGLLLAAADQRHSYPHCWRCKHPVLFRSTPQ
                     WFISIDANELRRRSLEQIHSTRWIPAHGEARIAGMIESRPDWCISRQRTWGVPLPAVI
                     CSRCASRDSSPPFVNDAAFFAHVERLFLDEGSNAWFGEPDGEGGHRPYPSPGARVERL
                     VPAGVVCPDCGGRDGLQIDEHVVDVWFESGVSHSAVLGRRDELPWPAALYLEGHDQYR
                     GWFHSSLLVAVNDRDRAPYRGVLTHGFTLDGQGRKMSKSLGNVISPIDVAEKRGAEIL
                     RMWVSMVDFLEDLKLSDEILDRIAEAYRKVRNTFRYLLGNLSGFEPQHDAVPYERMGE
                     LDRWALQQLEQLRRKLVEAYESHQYHLVYHGLHQFCAVTLSSFYLDIIKDRLYTMPRR
                     HPARRSALTVLHRLADSLARLMAPVLCFTAEEVWQELGTLSGVERWSGASVHTQRFPE
                     PLPLAADQQLIERWTRFLRLREEISKALEIARQEKRIGTALEAHVVIEAGDPRVAEFL
                     RSFGDELRFLLITSGVSFGPVGGGAFRSAVEPGLAVEVRRADGDKCQRCWNYTTDVGQ
                     DASWPGVCLRCAAHVRQIVEQADPA"
     gene            3376..3960
                     /gene="lspA"
                     /locus_tag="VD788_02845"
     CDS             3376..3960
                     /gene="lspA"
                     /locus_tag="VD788_02845"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXV75228.1"
                     /translation="MSRLSAVRARWPYFAVAVGVVALDQLTKVLAHAYLRVSSAVVIV
                     PGCFNLSYSRNRGGLFGYFSELGSTWRVLLLTVLPVVAIALIGTFIVRTEPAHRSTRV
                     GLGLILGGAVGNLIDRLVRGEVVDFLDVYVSSTGAAGWLVERFGTAHWPTFNLADSAI
                     VVGAGLLILDMFRGQPASDTGADRVRPASDHGTR"
     gene            3970..4806
                     /locus_tag="VD788_02850"
     CDS             3970..4806
                     /locus_tag="VD788_02850"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HXV75229.1"
                     /translation="MFPILIDAGRHDLPLLGETRLFLPTYGVLFAAGVVLAWVWFIRR
                     TRGMGLPEERLFNLTFYSVLAGIIGAKLLLIAIDWRVYLSNPGEILGTLRSAGVLMGG
                     VFAAAITFYVYCQRQGLPVWKLADAVAAPLVFAQAVGRLGCHSAGCCWGVGVDAGHPL
                     AVVFRDPRAREQTGVPLDTPLFATQLTEMAFDALLAVALTLIWRRGVRPAGTVFWIYV
                     LAYSVGRGVIELWRGDVHRGLFFGGAVSTSQLIAAATAAVALAALIRGRGVLARDHAE
                     AR"
     gene            4803..5780
                     /locus_tag="VD788_02855"
     CDS             4803..5780
                     /locus_tag="VD788_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXV75230.1"
                     /translation="MIAGAGGETLRFAVPLEADGVRLDRFLTERIAGTTRASVGRLIR
                     ADRVRVDERPAKKTGLALKPGMTIRVDLPQPVTGAVAAERIPFDVIYEDDELLVVDKP
                     AGLTVHPGHGRPSGTLVNGLLGRGIALSPVGAPDRPGIVHRLDRETSGLLVVAKTERA
                     HRALAHDFAERRVAKRYRALVWGHPRPADGTVEREIGRSRGNPTRMTVHATRGRRRSA
                     RTHYRTIESLPGFGLLDVELETGRTHQIRVHLESIGHPIVGDERYGGRGWRGIQHPLK
                     RKAVREFDRLALHAVGLAFRHPGTGRELRFRVGVPPAFARLLVVLREHG"
     gene            5777..6709
                     /locus_tag="VD788_02860"
     CDS             5777..6709
                     /locus_tag="VD788_02860"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HXV75231.1"
                     /translation="MSRAGRATLAIVGTGRLAAALAAAAAASRWPVAVVAGRRRQPIA
                     EVKRTARAACGTRSPARAARLGEVVLLAVTDDAIESVAQRLAEANVEFAERVVLHHAG
                     ALGPEALAPLARRGAAVGVLHPLQALGSGRGPVGLLRGARVRIEGHARAVAVARRLAR
                     AFGMVPLRLTRQLGARDRVAYHAAASLASNDLVALFATAIEVFESIGTTRGEAIAALT
                     PLVRATLEQLAAAGPRGALTGPVARGDVETLERHLDELPRRVPEAAEIHRVLSLRLLA
                     IAERLHPGQASEARRRIRRLLDPVRAGRRRGPAV"
     gene            6751..7371
                     /locus_tag="VD788_02865"
     CDS             6751..7371
                     /locus_tag="VD788_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75232.1"
                     /translation="MPGRRHRRVVPLLLVLTVATGLGPRAEPPADPPQTKPQETFTEW
                     GRSTRTVVPPTEAGVWDGTWYYVSRDALIVLWMRTVEDQPQVKLQYRNQGTGEGFVTD
                     WEGNADYRFREAEARFRLNVDRRDPYVITGRWDWQLDSGKGRRIEEGDYSIYRTGDGR
                     FLVVDFADFSRRLESSAGPDRVAASTPSWTFTKASKRLVLWDELPF"
     gene            7395..8873
                     /locus_tag="VD788_02870"
     CDS             7395..8873
                     /locus_tag="VD788_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HXV75233.1"
                     /translation="MRSRPVLRRTPLVALVLVCAIAGASPAGDAAGDPDRRFATRVIK
                     VDGPLGSPAVRSHVRFSLSIGFNAVWVPSRFAAAWTAADAPDGPRLEPEFLALARESR
                     SSGVGLLLVMEPVRDHDGSFVFSDERQAARLDKLMRIARRDAGVHDFVLSFFDTPLQL
                     TELGDRLDYGPSAARAQVALARALARRLRGDDRLWLEPSVTSDRFLGDPELPYSVALL
                     EALDGLDRRVGIVWSGPQPISPELTVEQVRAAETRFGGRKLLFDDRYPANGSEPRIPL
                     ALVLGPLRARAPAVADRIDTYAVTPMTELGASRLALLTIADFLTDPDGYDADASWERA
                     KRRLAGEDERAYEALRTQAAEWGGWVGTRNYHTAADENPRTAAASLADPAAFAAWIWP
                     VRTYPGRIDELAGLDDTLFRDELLLTMARRLAVARAAAPARRLLVPETSVEEAAEARR
                     SIERQRAGLSGEPAVRVALDRFLVHAGLGALIADGPPPVPLP"
BASE COUNT         1171 a         3186 c         3272 g         1412 t
ORIGIN      
        1 tctcggcgac gacgatccgc cgcagcccgt cgggcgcgcg gtcgcacacc ccgcggatcg
       61 cggcggcgag ggcctcggat ccgccgctgg tgaagacgac ctcggacgcg cgcgcgccga
      121 cgagctcggc cacctcggcc cgcgcgtcct cgatcagcgc gcgtctcgcg cgcccctcgg
      181 cgtgcagcga cgacgggttg gcttgcgggc cggccagcgc cgccagcatc acctccttcg
      241 cttcgggccg cagcgggctc gtcgcgttgt gatcgagata gactcgcgtc gggtcggtca
      301 tcgcgtccca tcttagccac ttttgcggcc cactctcccc gtcgggcggg tgtgttacat
      361 tacgcggtcc agccgaggcc gccaagtgtc aaagaacgtt gaacttaaga gcacgctcaa
      421 cctgccgagt acccggttct cgatgaaggc gaacctgccg cagaaggagc cggagatcct
      481 gacgtggtgg gactcgatcg atctgttcga ccgcatccgc cgggcgcgcg ccggcgacgc
      541 gccctacgtg ctgcacgacg ggccgccgta cgccaacggc aagatccacc tcggtcaggc
      601 gatgaacaag atcctcaagg acttcgtcgt caaatcgcac acgatgagcg gccgcaacgc
      661 ggtctacgtg ccgggctggg attgccacgg gctgccgatc gagcaccggg tcgacaagga
      721 gctcggtccg cgcaaggcgc ggatgacgcc gctcgagatc cgcgcgcgct gtcgcgccta
      781 cgccgagaag ttcgtcgcgg tgcagggcga cgagttccgg cggctcggcg tcgtctggga
      841 ccggcgttcg gagctcgccg agcgggcgga ggaccgggcg acgcgcaact cgatctatcg
      901 cacgatcgac cacgcctacg aggccgagat catccgccag ctcgccgggt tcttcacccg
      961 cggagcgatc tatcacgggg tcaaaccggt ccactggtgc tggtcgtgcc gcacggcgtt
     1021 ggccgaggcc gaggtcgagt acgccgatcg caccgacccg tcgatctacg tcaagttccc
     1081 ggtcgacggg ctcgaccgcc gcgtgcccgc gctcgccggt cgcgacgtcg cggtcgtgat
     1141 ctggacgacg acgccgtgga cgctaccggc caacatggcg gtggcgttgc accccgagct
     1201 gacctacgtc gcgatcgagg tcggccgcga gacgctgatc gtcgcggaag gcctgctcgg
     1261 gtcgctcggc gcgacgctgg gctgggacgc tccgcgcgag gtcgcccggt tcaccgggcg
     1321 cgagctggtc ggcaccggcg acgactgggt cggacacgac gcaccggtgc ggcggcccta
     1381 ctcgacgccg accgaacccg cgggcggcgg cggcgtgttg atcctcggcg agcacgtcac
     1441 gctcgacgcc ggcacgggct gcgtccacac cgcgcccggg cacggtgccg aggacttcca
     1501 cgtcggcaag cagtacgggc tcgacgtctt caatccggtc gacgacgagg gacggttcat
     1561 cccgtcgaag gtcaccgagg agtggctcgc gggcgcgttc gtgctcgacg cgaatcgctc
     1621 gatcgtcgag gacctggacc ggcgcgggtt gctgctggcc gcagccgacc agcggcacag
     1681 ctacccgcac tgctggcgtt gcaagcaccc cgtgctgttc cgctcgacgc cgcagtggtt
     1741 catctcgatc gacgccaacg agctgaggcg gcgctcgctc gagcagatcc actcgacgcg
     1801 ctggatcccg gcccacggcg aggcgcggat cgccgggatg atcgagtcgc ggcccgactg
     1861 gtgcatctcg cggcagcgga cctggggcgt gccgctcccg gcagtgatct gctcgcgctg
     1921 cgcctcgcgc gattcgagcc cgccgttcgt caacgacgcg gccttcttcg cgcacgtcga
     1981 gcgactgttt ctcgatgagg gctcgaacgc ctggttcggc gagccggacg gcgagggcgg
     2041 gcaccggccc tacccgtcgc cgggagcgcg ggtcgagcgg ctggtgccgg ccggggtcgt
     2101 ctgccccgac tgcggcggcc gcgacggcct gcagatcgac gagcacgtcg tcgacgtctg
     2161 gttcgagtcc ggggtgtcgc actccgccgt gctgggacgt cgcgacgagc tgccgtggcc
     2221 ggccgccctc tacctcgagg ggcacgacca gtaccgcggc tggttccatt cctcgctgct
     2281 ggtcgcggtc aacgaccgcg accgtgcgcc gtaccgcggg gtgctgaccc acggcttcac
     2341 cctcgacggg caggggcgca agatgtcgaa gtcgctgggc aacgtgatct cgccgatcga
     2401 cgtcgccgag aagcggggcg ccgagatcct gcgcatgtgg gtctcgatgg tcgacttcct
     2461 cgaggacctg aagctgtccg acgagatcct cgatcgcatc gccgaggcct accgcaaggt
     2521 gcgcaacacc ttccgctacc tgctgggcaa cctgagcggc ttcgagccac agcacgacgc
     2581 ggtaccgtac gagcggatgg gcgagctcga ccgctgggct ctgcaacagc tcgaacagct
     2641 ccggcgcaag ttggtcgagg cctacgagtc gcaccagtac cacctcgtgt accacgggct
     2701 gcaccagttc tgcgcggtca cgctgtcctc gttctatctc gacatcatca aggatcggct
     2761 gtacacgatg ccgcggcgcc atccggcgcg acgctcggcg ctgaccgtgc tccaccggct
     2821 ggccgactcg ctggcgcggc tgatggcacc ggtgctgtgc ttcaccgccg aggaggtctg
     2881 gcaggagctg ggcacgctgt cgggggtcga acgctggtcc ggcgcgagcg tacacaccca
     2941 gcggttcccc gagccgcttc cgctggccgc ggatcagcag ctgatcgagc gctggacacg
     3001 cttcctgcgg ctgcgcgagg agatctcgaa ggcgctcgag atcgcgcgcc aggagaagcg
     3061 gatcggcacc gcgctcgagg cgcatgtcgt gatcgaggcc ggcgatccgc gggtggccga
     3121 gtttctccga tcgttcggcg acgagctgcg cttcctgttg atcacgagcg gcgtttcgtt
     3181 cggcccggtc ggtggcgggg cgttccgctc ggccgtcgag ccgggcctgg cggtcgaggt
     3241 gagacgcgcc gacggcgaca agtgccagcg ctgctggaac tacacgaccg acgtcgggca
     3301 ggacgcgagc tggcccggcg tctgcctgcg ctgcgcggct cacgtgcgcc agatcgtcga
     3361 acaggccgac ccggcatgag ccggctgtcc gcggtccgtg cccgctggcc ctacttcgcc
     3421 gtcgcggtcg gcgtcgtcgc cctcgatcag ctgaccaagg tgctggccca cgcctatctt
     3481 cgcgtctcgt cggccgtcgt gatcgttccc ggctgcttca acctgtcgta ctcgcgcaac
     3541 cgcggcgggc tgttcggcta cttctccgag ctcggcagta cgtggcgcgt actgctgctg
     3601 accgtactgc cggtcgtcgc gatcgcgctg atcggcacgt tcatcgtgcg gaccgaaccg
     3661 gcgcaccgca gcacgcgggt cgggctgggg ctgatcctcg gcggcgccgt cggtaacctg
     3721 atcgaccggt tggtccgcgg cgaggtggtc gacttcctcg acgtctacgt ttcctcgacc
     3781 ggcgcggccg ggtggctggt cgagcggttc ggcacggcgc actggccgac gttcaatctg
     3841 gccgactcgg cgatcgtcgt cggtgccggc ttgctgatcc tcgacatgtt ccgcgggcaa
     3901 cccgcgagcg atacgggtgc ggatcgcgtg agaccggcga gcgaccacgg aacgcgctag
     3961 ggtgatcccg tgtttcccat cctgatcgac gccggtcgcc acgacctacc gctgctcggc
     4021 gagacgcggc tgttcctgcc gacctacggc gtgctgttcg ccgcgggcgt ggtgctggcc
     4081 tgggtctggt tcatccgacg tacgcgcggg atggggctcc ccgaggagcg gctgttcaac
     4141 ctgacgttct actcggtgct ggccggcatc atcggtgcca agctgctgtt gatcgcgatc
     4201 gattggcgcg tctatctgag caacccgggc gagatcctcg gcacgttgcg ctccgccggc
     4261 gtattgatgg gcggcgtctt cgccgccgcg atcacctttt acgtctactg ccagcgacag
     4321 ggattgccgg tgtggaagct ggccgacgcg gtcgcggcac cgctggtctt cgcgcaggcg
     4381 gtcgggcggc tcggctgtca ttccgccggc tgttgctggg gcgtcggcgt cgacgccggc
     4441 cacccgctcg ccgtcgtctt ccgcgacccg cgcgcccgcg agcagaccgg cgtaccgctc
     4501 gacacgccgt tgttcgcgac ccagctgacc gaaatggcgt tcgacgcgct gctcgccgtg
     4561 gcgctcacgc tgatctggcg ccgcggcgta cggccggcgg gaaccgtctt ctggatctac
     4621 gtcctggcct acagcgtcgg gcgcggcgtg atcgagctct ggcgcgggga cgtccatcgc
     4681 gggctctttt tcggcggcgc ggtctccacc tcacagctga tcgccgcggc caccgcggcg
     4741 gtggcgctcg cggcgttgat tcgcggtcgc ggcgtgctcg cccgggacca cgccgaggcg
     4801 cgatgatcgc gggcgccggc ggcgagacgt tgcgcttcgc cgtcccgctc gaagccgacg
     4861 gcgtacggct cgatcggttc ctcaccgagc gcatcgccgg tacgacccgc gcgtcggtcg
     4921 gccgcctgat ccgcgcggac cgtgtgcggg tcgacgagcg gcccgcgaag aaaacggggc
     4981 tcgcgctcaa accggggatg acgattcgcg tcgatctgcc gcagcccgtg accggcgcgg
     5041 tcgcggccga gcggatcccg ttcgacgtga tctacgagga cgacgagctg ttggtggtcg
     5101 acaagccggc cggactgacc gttcatcccg gtcatggccg gccgagcggc acgttggtca
     5161 acgggttgct cggccgtggg atcgcgctct ccccggtcgg cgcgcccgat cgacccggta
     5221 tcgtccatcg cttggaccgc gagacctccg ggctgctcgt cgtggccaag accgagcgcg
     5281 cccaccgggc actggctcac gacttcgccg agcggcgggt cgccaagcgt taccgtgcgc
     5341 tggtctgggg gcatccgcga ccggccgacg ggacggtcga gcgggagatc gggcggagtc
     5401 gcggcaaccc gacgaggatg acggtgcacg cgacgcgcgg gcgccggcga tcggcgcgca
     5461 cgcactaccg cacgatcgaa tcgctgccgg gcttcggcct gctcgacgtc gagctcgaga
     5521 ccgggcgtac gcatcagatt cgcgtccacc tcgagtcgat cggccacccg atcgtcggcg
     5581 acgagcgcta cggcgggcgc ggctggcgcg gcatccagca cccgctcaag cgcaaggccg
     5641 tccgcgagtt cgaccggctg gcgctgcatg ccgtggggct cgccttccgc caccccggga
     5701 ccggccgcga gctgcggttt cgcgtcggcg ttccaccggc gttcgcacgg ctgctcgtgg
     5761 tgctacggga gcacggatga gccgcgccgg gcgggcgacg ctcgcgatcg tcgggaccgg
     5821 ccgcctcgcc gcggcgctcg ccgcggccgc cgcggcgagc cgctggccgg tggccgtcgt
     5881 ggccggccgc cgccggcaac cgatcgcgga ggtcaagcgc acggctcgcg cggcctgcgg
     5941 tacccgctcg ccggcgcgcg ccgcacgcct cggcgaggtg gtgctgctgg cggtgaccga
     6001 cgacgcgatc gaatcggtcg ctcagcggct ggccgaggcg aacgtcgagt tcgccgagcg
     6061 cgtcgtgctg catcacgcgg gcgcgttggg ccccgaagcc ctggcgccgc tcgcgcggcg
     6121 gggcgcggcg gtcggcgtcc tgcacccgtt gcaagcgctc gggtccgggc gcggaccggt
     6181 cgggctgttg cgcggcgcgc gggtgcgtat cgaggggcac gctcgcgcgg tggcggtcgc
     6241 gcgacgactg gcgcgcgcgt tcggcatggt gccgctgcgc ctgacgcgac agctcggtgc
     6301 tcgcgaccgc gtggcctacc acgcggccgc gtcgctcgcg agcaacgacc tggtcgccct
     6361 gttcgccacc gcgatcgagg tgttcgagtc gatcggcacg acccgtggcg aggcgatcgc
     6421 ggccttgacc ccactggtcc gcgccacgct cgagcagctg gcggcggcgg gtccgcgcgg
     6481 tgcgctgacc ggaccggtcg cgcgcggcga cgtcgagacg ctcgagcgtc acctcgacga
     6541 gctgccgcgg cgcgttccgg aggccgccga gatccaccgc gtgttgtcgt tgcggctgct
     6601 cgcgatcgcc gagcgtctgc atccggggca ggcgtccgag gcgcggcgtc gcatccggcg
     6661 cttgctcgat ccggtccgcg ctggtcggcg gcggggtccg gcggtatagt tagcggtcgg
     6721 gcccacgggg ctgttgggag ccgttgcgcg atgcctggac gccgacaccg gagggtcgtt
     6781 ccgctactgc tggtgctgac cgtggccacc gggctcggac cgcgcgccga gccaccggcc
     6841 gacccgccgc agaccaagcc gcaagagacg ttcaccgagt ggggacggtc gacccgcacg
     6901 gtcgtaccgc cgaccgaggc cggcgtgtgg gacggcacct ggtattacgt cagccgcgat
     6961 gcgctgatcg tgttgtggat gcgcaccgtc gaggaccagc cgcaggtcaa gctgcagtac
     7021 cgcaaccagg ggacgggcga gggcttcgtc accgactggg agggcaacgc cgactaccgg
     7081 tttcgcgagg ccgaagcccg cttcaggttg aacgtcgacc ggcgtgaccc ctacgtcatc
     7141 accggtcgct gggattggca gctcgactcc ggcaagggtc gacggatcga ggagggtgac
     7201 tactcgatct accgcaccgg cgacggccgc ttcctcgtgg tcgacttcgc cgacttcagc
     7261 cgtcgtctcg agagcagcgc tggaccggac cgggtcgcgg cgagcactcc gtcatggacc
     7321 ttcaccaagg cttcgaaacg cctcgtgctg tgggacgagc tgccgttctg atccctcatc
     7381 ggactcgacg ttcgatgcgc tcccgcccgg tcctccgtcg cacgccgctc gtcgcgctcg
     7441 tgttggtctg tgcgatcgcg ggggccagtc ccgcgggaga cgctgcgggc gaccccgatc
     7501 gtcggttcgc cacccgggtg atcaaggtcg acgggccgct cggaagtccg gcggtccgga
     7561 gccacgttcg cttcagcctg tcgatcggct tcaacgcggt ctgggtgccg tcgcgattcg
     7621 cggcggcctg gacggccgcg gacgcgccgg acggtccgcg gctcgagccc gagttcctcg
     7681 ccctcgcgcg ggaatcacgc tcgagcggcg tcggtttgct gctggtgatg gaaccggttc
     7741 gcgaccacga cggtagcttc gtcttttccg acgagcgcca ggcggcacgg ctcgacaagc
     7801 tgatgcgtat cgcgcggcgc gacgccggtg tgcacgactt cgtcctgtcg ttcttcgaca
     7861 cgccgcttca gctgaccgag ttgggcgacc ggttggacta cggtccctcg gccgctcggg
     7921 ctcaggtcgc cctggcgcgc gcgctcgcgc gccggctgcg cggggacgac cgcttgtggc
     7981 tcgaaccgtc ggtcacgagc gatcggttcc tcggcgaccc cgagctcccc tactcggtcg
     8041 cgctgctcga ggcgctcgac ggactcgacc ggcgcgtcgg catcgtttgg agcgggccgc
     8101 agccgatctc gccggagctg acggtcgaac aggtccgcgc ggccgagacg cgcttcggcg
     8161 gacgcaagct gttgttcgac gatcgctacc cggcgaacgg ctcggagccg cggatcccgc
     8221 tggcgttggt gctcggcccg ttgcgcgcgc gcgcgcccgc cgtcgccgac cgcatcgata
     8281 cctacgccgt cacgccgatg accgagctcg gcgcgtcgcg cctggccttg ctgacgatcg
     8341 ccgacttcct gaccgacccc gacggctacg acgccgacgc gagctgggag cgggccaagc
     8401 ggcgcctggc cggcgaggac gagcgcgcct acgaggcgtt gcgcacgcag gctgccgagt
     8461 ggggtggttg ggtcgggacg aggaactacc acacggcggc ggacgagaac ccgcgcaccg
     8521 cggcggccag cctggccgac ccggcggcct tcgccgcgtg gatctggccg gtgcgcacct
     8581 atcccggacg gatcgacgag ctggccgggc tcgacgacac gctgttccgc gacgagctgc
     8641 tgctgacgat ggcccggcgg ctggccgtcg cccgcgcggc ggcgccggcc agacggctgc
     8701 tcgtgccgga gacctcggtc gaggaggcgg ccgaggcccg tcgttcaatc gagcggcagc
     8761 gcgccggtct gagcggcgag cccgcggtgc gggtcgcgct cgatcgtttc ctggttcacg
     8821 ccgggctcgg ggcgctgatc gccgacgggc cgccgccggt accgttacca tgagcgccgc
     8881 cagggtcggc ccgctgcgcc gctacgcccg ctggctccac accgactggc cggcgggcaa
     8941 ggtcgagaag ctgcccgagg tgcgcgacga cggcacgacg aacctcgcgg gtgtctacgt
     9001 cgtcggcgac ctgaccggcg taccgctgct caagttctcg g
//
LOCUS       DASPGN010000101         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_135161, whole genome shotgun sequence.
ACCESSION   DASPGN010000101 DASPGN010000000
VERSION     DASPGN010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135161"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VD788_02875"
     CDS             <1..357
                     /locus_tag="VD788_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75234.1"
                     /translation="LGAVAQALNGLLDTHEEARGRAQSRETRLRELLVGCVAADGSPA
                     AVVSLGGDVVASTFTDAETGTIVAAASELRGAVPDDLLGAGAVERVVDCGGKAVRFHL
                     LVVEGKRAVGWSVRSS"
     gene            535..1887
                     /locus_tag="VD788_02880"
     CDS             535..1887
                     /locus_tag="VD788_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75235.1"
                     /translation="MKPIRLIRGIGLFGLAIVLSVSAFAQDRECDTITFTGFAHGDTI
                     GSLSLFGGDITLVFSADRFPGNAVPVTAYDTELWSLPGNPPPNDTHYDTQRAILCNPA
                     NNGGQGECMGIMAVIPDENFAIGGDDTEGGTITITGFTGDFEITNYDAVDTDDPSKRI
                     ILRVGPTLDFVGESSGLGNATVETVDTLPHTFSGQAQFEFLGSGGIDNIEICRDTPPA
                     SNCRVTAGGNRDGVFCPVLPNGDPDPDFCEEDQTSDDITHTWGGQAGAPPRVDGNWTH
                     KYLDRLARPHNKFTFHSNEVVFIQCSDPGFCDPARPAPARQIDFTGIGSFTNTSGVFR
                     DAPDGDLCYMVHLEDIGEPGPGGQLPDPPGPCTHCPGTAIEDEDCTNCTDYYEIEIYA
                     NATYDASNGQCLGQLIWRNGDPSMTCDGGDVFVAPNAPYEGFFTRTGNVQLHPDNNGP
                     "
     gene            2046..>2379
                     /locus_tag="VD788_02885"
     CDS             2046..>2379
                     /locus_tag="VD788_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75236.1"
                     /translation="MKARVALMTTLLAVAPLSPSLAQDGTDAYIELLRSDLRADKQAI
                     VTYAMDLSDADSELFWPIYKAYETERTVLGDRMLELILKYPDAVEVTGPDTVRDVAAD
                     WFKLQEDRV"
BASE COUNT          408 a          792 c          790 g          389 t
ORIGIN      
        1 ctgggcgccg tggcccaggc gctgaacggt ctgctcgaca cccacgaaga ggcgcgcggt
       61 cgggcgcaat cgcgcgagac ccggttgcgc gagctgctcg tcgggtgcgt ggcggcggac
      121 gggtccccgg cggcggtcgt cagtctcggc ggcgacgtag tcgcgtcgac cttcaccgac
      181 gccgagacgg gcacgatcgt cgccgccgcg tccgagctgc gcggcgcggt tccggacgac
      241 ctgctcgggg ccggcgccgt cgagcgggtg gtcgactgcg gcggcaaggc ggtccgtttc
      301 cacctgctcg tcgtcgaagg caaacgcgcg gtcggctggt cggtccggtc gagctgaggc
      361 gagccgccgc gaatccgggt tttcgggctt ttccggccgg tgatcgcgaa ttccggacgg
      421 aacacctcgt tgacaacagc cgggcttcgt gggatattcc ccgcaaaggc gttttcgtgc
      481 tcggcacggg tgcttgtctg cagggtccga tggggcttct gcgtggggga aaagatgaaa
      541 ccgattcgtt tgattcgcgg gatcggtctg ttcggtctcg cgatcgtgct gtccgtgtcc
      601 gcgttcgcgc aagaccgcga gtgcgacacg atcaccttca cgggcttcgc tcacggcgac
      661 acgatcggga gcctgtcgct gttcggtggc gacatcacgt tggtcttcag cgccgatcgc
      721 ttcccgggca acgccgtgcc ggtgacggcc tacgataccg agttgtggag cctgccgggc
      781 aacccgccgc cgaacgacac gcactacgac acccagcgtg cgattctgtg caacccggcc
      841 aacaacggcg ggcagggcga gtgcatgggg atcatggcgg tgattcccga cgagaatttc
      901 gccatcggcg gtgacgacac cgagggcggg acgatcacga tcaccggctt caccggcgac
      961 ttcgagatca cgaactacga cgccgtcgac accgacgatc cgtcgaagcg aatcatcctc
     1021 agggtcggac cgacgctcga tttcgtcggc gaatcgtccg ggctgggcaa cgcgaccgtc
     1081 gagaccgtcg acacgctgcc ccacaccttc agcggacaag cgcagttcga gttcctcggc
     1141 tcgggcggca tcgacaacat cgagatctgc cgcgatacgc cgccggcctc gaactgtcgc
     1201 gtcaccgccg gcggcaaccg cgacggcgtc ttctgtccgg tgttgccgaa cggcgacccc
     1261 gatccggact tctgcgaaga ggaccagacg agcgacgaca tcacccacac ctggggtggg
     1321 caggccggag cgccgccgcg cgtcgacggc aactggacgc acaaatacct cgatcggctg
     1381 gcacgaccgc acaacaagtt caccttccac tcgaacgagg tggtgttcat ccagtgcagc
     1441 gatcccggct tctgtgaccc ggcgcggccg gcaccggcgc ggcagatcga cttcaccggt
     1501 atcggcagct tcaccaatac ctcgggcgtc ttccgcgacg cgccggacgg cgatctgtgc
     1561 tacatggtgc acctcgagga catcggcgag ccgggccccg gcggacagct gccggatccg
     1621 cccggaccgt gcacgcactg cccggggacg gcgatcgagg acgaggactg caccaactgc
     1681 accgattact acgagatcga gatctacgcg aacgcgacct acgacgcctc gaacggtcag
     1741 tgtctcggtc agttgatctg gcgcaacggc gatccgtcga tgacctgcga cggcggcgac
     1801 gtgttcgtcg ctccgaacgc accgtacgag ggcttcttca cgcgtacggg gaacgtccag
     1861 ctgcacccgg acaacaacgg accctgatcc caccacgagt ggacggtcga agaaggggcg
     1921 agcgatcgcc ccttcttttt tcctgtggcg cgatcgaccg aagaaacccg ggtcgttgct
     1981 tcggctccac ggccgtagta cgatctcgcg ccgcacggga cgtgtccgtc ccaccggagg
     2041 tgatgatgaa agcccgcgta gcgctgatga cgactctgct ggccgtggcg ccgctgtccc
     2101 cgtcgctggc ccaagacgga accgacgcct acatcgagct gcttcgttcc gacctgcgtg
     2161 ccgacaagca ggccatcgtg acctacgcga tggacttgag cgatgccgac tccgagctgt
     2221 tctggccgat ctacaaggcc tacgagaccg aacgcacggt gctcggtgac cgcatgctcg
     2281 agttgatcct caagtacccc gacgccgtcg aggtgacggg cccggacacc gtgcgggacg
     2341 tggccgcgga ctggttcaag ctgcaggaag accgcgtga
//
LOCUS       DASPGN010000102         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_135316, whole genome shotgun sequence.
ACCESSION   DASPGN010000102 DASPGN010000000
VERSION     DASPGN010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135316"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VD788_02890"
     CDS             complement(<1..882)
                     /locus_tag="VD788_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXV75237.1"
                     /translation="MKTIARVVSSTVAAALLSSLAAAAQQDRPGGATQAPPGHVTVLP
                     APDFRYSGSIGRTIDESDPPEFPQPARPPEGAPNIVYILIDDAGYGQFGTFGGQIETP
                     ALDRVAADGLRYTRFHTTALCSPTRAALLTGRNHHSTGTGVITEAATGYDGYTGMIGK
                     NLGTIAEVLRQHGYATSWFGKNHNTPDWETSVNGPFDRWPSGLGFDYFYGFMGGDMDQ
                     FQPVLYENHNLVPRSTDPDYILTKDLVDKAITWLRRVRSISPDKPYFLYMSTGATHAP
                     HHVSPEYLPRFRGKFDQG"
     gene            1009..2178
                     /locus_tag="VD788_02895"
     CDS             1009..2178
                     /locus_tag="VD788_02895"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXV75238.1"
                     /translation="MFSSRLPGDLSPNALARLRASSAEVPYDLTASDPTRSGVAYPAG
                     LLAPLADPRGLRYRPDPLGLVEARQAVARIYTARGLAVDPERVVLTASSSEAYSLLFK
                     VLGDPGDAVLLPQPSYPLFAHLAALDGLQARPYRLVPEDGWQPDCTEPFDPRARLLLV
                     VHPNNPTGTFVAPEVLAALDRRCAAADVALVVDEVFLDYPLDGTPRRSALAGSHDALT
                     FVLGGLSKSCGLPQLKLSWVVLAGPTDRVERAVERLEFAADQYLSVATPVQLALPRLL
                     TAGAEVRHALAERCHANLATLRERVARRSIVSLVVPEGGWSAVLRLPASVDEERFACD
                     LLEHHGVAVHPGAPFGFPRPGYFVLSLLPRPEIFAAGIERLLTAAAGALADPGCN"
BASE COUNT          300 a          837 c          818 g          387 t
ORIGIN      
        1 gccctggtcg aacttgccgc ggaaccgcgg caggtattcg ggcgagacgt ggtgcggggc
       61 gtgagtggcg ccggtggaca tgtagaggaa atacggcttg tcgggcgaga tggagcgcac
      121 gcggcgcagc caggtaatgg ccttgtcgac caggtccttg gtcaggatgt aatccgggtc
      181 ggtcgagcgc ggcaccaggt tgtggttctc gtagagcacc ggctggaact ggtccatgtc
      241 gccgcccatg aagccgtaga agtagtcgaa gcccaggccg ctcggccagc gatcgaacgg
      301 tccgttgacg ctcgtctccc agtcgggcgt gttgtggttc ttgccgaacc acgaggtggc
      361 gtagccgtgc tggcgcagca cctcggcgat cgtgccgagg ttcttgccga tcatgccggt
      421 gtagccgtcg tagccggtcg cggcttcggt gatcacgccg gtgccggtcg agtgatggtt
      481 gcgcccggtc aagagcgccg cgcgcgtcgg cgaacagagc gcggtggtgt ggaaccgcgt
      541 gtagcgcagg ccgtcggccg ccacccggtc gagcgccggt gtttcgatct gtccgccgaa
      601 cgtgccgaac tgtccgtacc ccgcgtcgtc gatcagaatg tagacgatgt tcggggcgcc
      661 ctcaggcggc ctggccggct gcgggaactc cggcggatcg ctctcgtcga tcgtccgacc
      721 gatcgaaccg ctgtagcgga agtccggcgc cggcagcacc gtgacgtggc ccggcggcgc
      781 ctgcgtcgct cccccgggcc ggtcctgttg cgctgccgcg gcgagcgacg acagcaacgc
      841 tgccgcgacg gtcgacgaca ccactctcgc gatcgttttc atcttcgacc cctttccggc
      901 cggactggtg acacgcggaa gtgcgctact ctaccacgct ccgggcgtga cgaaagaagc
      961 gtcgtgaccg gcgcccgggc gcgttcgcga agagattcgg tggcgaggat gttctcgagt
     1021 cggctgcccg gcgacctatc gcccaatgcg ctggcgcgtc tgcgcgcgtc gtcggccgag
     1081 gtaccgtacg acctgacggc cagcgacccg acgcggagcg gagtcgcgta ccccgccggg
     1141 ctgctcgcgc cgctcgcgga tccgcgcggg ctgcgctacc ggccggaccc gctcggtctc
     1201 gtcgaagccc ggcaagcggt ggcccggatc tacaccgcgc ggggtctcgc ggtcgatccc
     1261 gagcgcgtcg tgctgaccgc gtcgtcgagc gaggcctact cgttgttgtt caaggtgctc
     1321 ggcgacccgg gcgatgcggt gctcctgccg caaccgtcgt atccgctgtt cgcgcacctc
     1381 gcggcgctcg acggcctgca ggcgcgaccc taccgcctcg ttcccgagga cggttggcag
     1441 cccgactgca ccgagccatt cgacccgcgc gcgcggctgc tcctcgtcgt ccacccgaac
     1501 aacccgacgg gcacgttcgt cgcgcccgag gtgctcgcgg cgctcgatcg gcgctgcgcg
     1561 gcggccgacg tggcgctcgt cgtcgacgag gtgttcctcg actacccgct cgacggaacg
     1621 ccgcgccgct cggcgctggc cgggtcgcac gacgcgttga cgttcgtcct cggcggtctg
     1681 tcgaagagct gcgggctgcc ccagctcaag ctgtcgtggg tcgtgctcgc cggcccgacc
     1741 gaccgcgtcg agcgggcggt cgagcgactc gagttcgccg ccgaccagta cctgtcggtc
     1801 gcgacacccg tccaactcgc cctgccgcga ctgctgaccg ccggcgccga ggtgcgacac
     1861 gcgctcgccg aacgctgtca cgcgaacctc gccacgctgc gcgagcgcgt cgcccggcga
     1921 tcgatcgtca gtctcgtcgt cccggaaggc ggctggagcg cggtgctgcg gctgccggcc
     1981 tcggtcgatg aagagcgctt cgcctgcgac ctgctcgagc accacggcgt cgccgtgcac
     2041 cccggcgcgc cgttcggctt tccgcgcccg ggctacttcg tgctgagcct gctgccccgc
     2101 ccggaaatct tcgccgccgg gatcgagcgc ctgctgaccg cggctgccgg cgcgctcgcc
     2161 gatcccgggt gcaactgacg acgcgtcgac ggccgcccgc gcgtcgtcag aactcggtct
     2221 ccttgaccac ccgtccgtcc ttgtcccaag tgcgccagat gcccgcctgc gctccctgtc
     2281 ggaactcgcc ggtgcgcatg acgctgccgt cgcggcggaa gaactcccac ttgccgtgca
     2341 tc
//
LOCUS       DASPGN010000103         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1657994, whole genome shotgun sequence.
ACCESSION   DASPGN010000103 DASPGN010000000
VERSION     DASPGN010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1657994"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VD788_02900"
     CDS             <1..351
                     /locus_tag="VD788_02900"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXV75239.1"
                     /translation="LADEGISLAVYDARFAKPVDLELLERLIGGDVPILTVEDHHLIG
                     GFGACVIEACHDRGLSTAGIHRLGLPDGWIYQGSRGQQLAEAGIDAAAIAERARRIVH
                     SLPRIAAKTRLRAG"
     gene            468..1991
                     /locus_tag="VD788_02905"
     CDS             468..1991
                     /locus_tag="VD788_02905"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXV75240.1"
                     /translation="MVRDGIVVTLILFATCAISCATSRPQTIPEPPAAEPGAVVEPSV
                     GVDSNVSPPPPPDLTPLYARLDPAHREYREGIELLSAGDEIGGEFKISNAANELRSIA
                     LECASSDGCEMDRVLDVVDELLSLQNVAIKQQAFHIEGMEASIAQDVEREPGTSSFDP
                     LPELGRTVSLLKGTDLRDLITLNGPVKAAIDDWLTWMRPMLMESWNNYQYLRDRVAPV
                     YEEAGLPEALLFAMMASETGVKAHSFSRAGAAGPLQFMRGTGQKYGLRVIDGFDTRLD
                     PVESTRANVAYLDDQFDVFNDSLEKVLAAYNGGENRMRTIHRRHDGASLWDSEVFYSL
                     PRETREYVPRILAAAWLFLHPEEYNLELPELLRTTVGLTLRQEISLGELTVCLGQTGN
                     PNGWFRTLRNLNPRFDPGERIAAGLTIEVPTAVVEPYERQCLAGELLAKARELHDANY
                     PDEPEMIVYTVRNGDTLSRIASRHSCVSLGELAALNDIRPPRYVIHVGQRVKIPACG"
     gene            complement(2018..>3048)
                     /locus_tag="VD788_02910"
     CDS             complement(2018..>3048)
                     /locus_tag="VD788_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXV75241.1"
                     /translation="GDDGYFQRFRLDDGPDGRPVELTNVVGYLPGRLAERDGQAVVVG
                     AHYDHLGFGWPDAHRQFEGQLHPGADDNASGVAVLLELARVFAAGEAPDRALVFVAFT
                     AEESGRLGSRHFVEHSGRLPAEAVRAMVNLDTVGRLGDGPVTVMSTGTAEEWPHIVRG
                     VGFVTGIAGRSVEDSLSASDEASFIEHGVPAIQLFTAAHADYHRPSDTADKVDRTGLV
                     KVATFAREIVSYLVGREEPLTVTIDGHVAPAGQPSAGRRVSFGIVPDFGFAGPGVRAD
                     SVVPDSPADRAGIRGGDVLTEVDGRPVADLRELAAILAGLAPGRSVGAVVERDGTRLE
                     LSVTLVAR"
BASE COUNT          475 a         1075 c         1065 g          433 t
ORIGIN      
        1 ctggccgacg agggcatctc gctcgcggtc tacgacgcgc gcttcgccaa gccggtcgac
       61 ctcgagttgc tcgagcgact gatcggcggc gacgtcccga tcctgacggt ggaggaccat
      121 cacctgatcg gcggcttcgg cgcgtgcgtg atcgaggcct gtcacgaccg tggcctgtcg
      181 acggccggca tccatcgcct cgggttgccc gacggctgga tctaccaggg gtcgcgcggc
      241 cagcagttgg ccgaggccgg gatcgacgcc gcggcgatcg ccgagcgcgc gcgccgcatc
      301 gtccacagcc tgccgcggat cgccgccaag acccggctgc gcgccggctg acgcgagccg
      361 tccggcagac gcggccaccc gagccgcagg aaaccacggc gcggctgccc gcgcccggtc
      421 gggcgcccta tacttacgct gttgcctgca cggggggaac gaggaagatg gtgcgtgacg
      481 gcatcgtggt gacgttgatt ctcttcgcga cctgcgcgat ctcgtgcgcg acgagccgac
      541 cgcaaacgat ccccgagccg cccgccgctg agccgggcgc cgtcgtcgag ccgtcggtcg
      601 gcgtggactc gaacgtgagc ccgcctcccc cgcccgacct gacgccgctg tacgcgcggc
      661 tcgaccctgc ccaccgggaa taccgcgagg gcatcgagct gctgtccgcc ggggacgaga
      721 tcggtggcga gttcaagatc agcaacgccg cgaacgagct gcgctcgatc gcactcgagt
      781 gcgcgtcgtc ggacggatgc gagatggacc gcgtgctcga tgtggtcgat gagctgctca
      841 gcctccagaa cgtcgcgatc aagcagcagg cgttccacat cgaagggatg gaggccagca
      901 tcgcccagga cgtcgaacgc gagccgggca ccagctcgtt cgacccgctg cccgagctcg
      961 gccgcacggt ctcgttgctc aagggcaccg acctgcgcga tctgatcacg ctcaacgggc
     1021 cggtcaaggc ggcgatcgac gactggttga cctggatgcg gccgatgctg atggaatcgt
     1081 ggaacaacta ccagtacctg cgcgatcggg tggcgccggt ctacgaggag gccggactgc
     1141 ccgaggcgct gctgttcgcg atgatggcgt cggagacggg tgtcaaggcg cactcgttct
     1201 cccgagccgg agccgcggga ccgctgcagt tcatgcgtgg caccggtcag aagtacgggc
     1261 ttcgggtgat cgacggcttc gacacgcggc tcgacccggt cgagtcgacg cgcgccaacg
     1321 tcgcctacct cgacgaccag ttcgacgtgt tcaacgacag cttggagaag gtgctggccg
     1381 cctacaacgg cggcgagaac cgcatgcgca cgatccaccg gcgccacgac ggcgccagcc
     1441 tgtgggacag cgaggtcttc tactcgctgc ctcgcgagac gcgcgaatac gtgccgcgga
     1501 tcctggccgc ggcctggctg ttcctccatc cggaggagta caacctcgag ctgccggagc
     1561 ttctacggac gacggtgggc ttgacccttc gccaggagat ctcgctcggc gagctgacgg
     1621 tctgcctcgg ccagaccggc aacccgaacg gctggttccg cacgctgcgc aacctcaacc
     1681 cgcggttcga tccgggtgag cgcatcgcgg ccgggttgac gatcgaggtt ccgaccgccg
     1741 tcgtcgaacc ctacgagcgg cagtgtctcg ccggcgagct gttggcgaaa gcccgcgagc
     1801 tgcacgacgc caattacccc gacgagcccg agatgatcgt ctacacggtc cgcaacggcg
     1861 acactctctc tcgcatcgcc tcccgtcaca gctgcgtctc cctcggcgag ctggctgccc
     1921 tcaacgacat ccgcccgccg cgctacgtga tccacgtcgg gcagcgggtc aagatcccgg
     1981 cctgcggctg acggctctca ccggcgaacg gcggggctca gcgcgcgacg agggtgacgg
     2041 acagctcgag ccgcgtccca tcgcgctcga cgacggcgcc gacggagcga ccgggcgcga
     2101 gcccggcgag gatcgccgcg agctccctca ggtcggcgac cggacggccg tcgacctcgg
     2161 tcagcacgtc gccgccgcgg atgccggcgc ggtcggccgg agagtcgggc acgaccgagt
     2221 cggcccgcac gcccggtccg gcgaacccga agtcgggcac gatgccgaag gagactcggc
     2281 gcccggcgga cggctggccg gccggcgcga cgtggccgtc gatcgtcacg gtcagcggct
     2341 cctcgcgtcc caccaggtag ctgacgatct cgcgggcgaa ggtcgccacc ttgaccaacc
     2401 ccgtccgatc gaccttgtcg gccgtatccg acggccggtg ataatcggca tgcgccgcgg
     2461 tgaagagctg gatcgcgggt acgccgtgct cgatgaaact cgcctcgtcg gatgcggaga
     2521 gcgagtcctc cacactgcgg ccggcgatgc cggtgacgaa gccgacgcca cggacgatgt
     2581 gcggccattc ctcggcggtg cccgtcgaca tcaccgtgac cgggccgtcg ccgagccggc
     2641 cgacggtgtc gagattgacc atcgcgcgga cggcttcggc cgggagccgt ccggagtgct
     2701 cgacgaagtg gcgagagccg agccgcccgc tctcctccgc cgtgaaggcg acgaagacca
     2761 gtgcgcggtc cggggcttcg cccgcggcga agacgcgcgc cagctcgagc agcaccgcga
     2821 cgccgctcgc gttgtcgtcg gcaccagggt ggagctgtcc ctcgaattgc cggtgcgcgt
     2881 cgggccagcc gaagccgagg tggtcgtagt gggcgccgac gacgacagcc tgcccgtccc
     2941 gctcggccag tcggccgggc aggtagccga cgacattcgt cagctcgacc ggtcggccgt
     3001 cgggtccgtc gtcgaggcga aaacgctgaa agtagccgtc gtcccccg
//
LOCUS       DASPGN010000104         9992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1189717, whole genome shotgun sequence.
ACCESSION   DASPGN010000104 DASPGN010000000
VERSION     DASPGN010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9992
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189717"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VD788_02915"
     CDS             <1..993
                     /locus_tag="VD788_02915"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV75242.1"
                     /translation="KRATEQRRLKAENVRLRDNVDRLVTPSQIVGESPQMRKVLELVE
                     SVAVTEATVLIRGESGTGKELIAHAIHTNSSRRYFPIIAVNCGALPESLLESELFGHE
                     KGAFTGAQFRRKGKLEMADGGTLFLDEVGTISLRTQVDLLRVLETKEFTRLGGTRPVR
                     VDFRVICATNQNLEHQVEQGQFREDLYFRLNVVTIQLPPLRDRREDIPQLAEHFAGKY
                     ARQMNRPFERIDPAAMDLLVRYDWPGNVRELANAIERALVVGKSSTIRADDLPLRLSQ
                     PCNGSSGDSLADIEREHIARMLEREGWNITHTASKLRIDRVTLYNKIKKYGLRR"
     gene            1010..1567
                     /locus_tag="VD788_02920"
     CDS             1010..1567
                     /locus_tag="VD788_02920"
                     /inference="COORDINATES: protein motif:HMM:NF033823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HXV75243.1"
                     /translation="MASPATVDLVPIYLTDYPRMTDAVGVALRERLGLRVRLRKPWFD
                     PEAAYDLARGQYSSRVLLELLLDDPDPGSARIVAITTIDLFAPVLTYVFGEAQLDGRA
                     AVVSLHRLRPEAYGLACDERLVRARLVTETVHELGHTFGLLHCNESSCVMHPSTYAEE
                     IDQKTDRFCDLCSSLLSRVSPPAIC"
     gene            1626..2135
                     /locus_tag="VD788_02925"
     CDS             1626..2135
                     /locus_tag="VD788_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HXV75244.1"
                     /translation="MIDSKLEKAINDQINKELQAWYSYLAMSAYCKKLNLSGFASFMD
                     GHAREEQAHAHRLMNYVLDRDGSIVLQRISAPAADFNSLLHLFELAVEQERSNTRSIY
                     ELYELAKQVNDYSTVAALQWFLDEQVEEEKVMTEALGLMRFAGDDKSALLTLNSQFGK
                     QATVETPSE"
     gene            complement(2150..2698)
                     /locus_tag="VD788_02930"
     CDS             complement(2150..2698)
                     /locus_tag="VD788_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HXV75245.1"
                     /translation="MVARAESFVALLRGINVGGRNKLPMKDLVRILERLGARNVRTYI
                     QSGNAVFEVAARGSSALARKLSAEIEKEFGFDARAQVLSLDAIDEAMMRNPFPEAESA
                     PASLHLGFLASLPKRPDLAKLDRLRQGSERFRLIGRVFYLHAPEGVGRSKLAAGAEQA
                     LGVPMTDRNWRTVCRVRELARD"
     gene            complement(2744..4810)
                     /locus_tag="VD788_02935"
     CDS             complement(2744..4810)
                     /locus_tag="VD788_02935"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HXV75246.1"
                     /translation="MVLPWRAMSQRQSTGVDGLERLLRERFGLTGFRPHQLEVCREVT
                     AGRDVLLVMPTGAGKSLCYQLPGIARGGTTLVVSPLIALMEDQANRLKARGFRAERIH
                     SGRDRGESQRVCRDYLDGALDFLFVAPERLGVAGFPEMLARRVPALVAVDEAHCISQW
                     GHDFRPDYRMLGERLPSLRPAPVIALTATATRLVQRDIVRQLGMRETGLFIHGFRRAN
                     IAIELAELRMSARQAMTERLLADPARRPAIVYAPTRRQAEELAGRLAVGFPAAPYHAG
                     LGGARRDEVQAAFLAGRLDVVVATIAFGMGIDKPDVRTIVHTALPGSVESYYQEIGRA
                     GRDGLPSRAVLLHGYADRRTHEYFLDRSYPDPRELERLRIELADVWRSREELLLATRL
                     DPERFDVALEKLWIHGGARVDPAGNARLGDDGWRQAYDAQRRHKRDELDLVTRFADSR
                     ECRMLHLVRHFGEREDGAGPCGSCDFCAPDSCVVLRFTAPDAGQSRALERIVSSLAER
                     DGQPTGRLHRELFGDGASRDECERLIAGLARAGVVRVREDSFERDGRLIRFRRVELTS
                     RGRASGLPADVPIPVPPDRGGSRAPRSTRSRTVPSGQTAVPAERAIEAPPAALVEALR
                     DWRLAEARRRGLPAFCILSNRDLTAIACSRPTDESSLLRVRGMGPKRVKDYGTAILSI
                     VNETSG"
     gene            complement(4822..5526)
                     /locus_tag="VD788_02940"
     CDS             complement(4822..5526)
                     /locus_tag="VD788_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV75247.1"
                     /translation="MTRQVALVTGAARGIGLEAVSQLVDRGHLVFAAVRVAGSTGIAD
                     LARDHPGRVHEVVMDVADDQSVAGAAGRVAELTDRLDLLINNAGIYPQDGGGIETLDL
                     GALLTAFQVNALGPLRVTRALLPLLRRGDGKRIVQVTSLMGSIGDNGSGGSYAYRMSK
                     AALNMANRNLAHELRPEGFVCVAVHPGWVRTRMGGSAAPLGLREAVEQLLDNACGASP
                     ADSGGLRGPGRRALPF"
     gene            complement(5523..6404)
                     /locus_tag="VD788_02945"
     CDS             complement(5523..6404)
                     /locus_tag="VD788_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV75248.1"
                     /translation="MRTDGACLAPGLLAGKTAWITGGGTGLGRAMALRFAGLGAAVGL
                     SGRRTEPLEETARQIVSAGGRALAAVCDVRDPTGVGAALDRVTQALGAPDVLVNNAAG
                     NFLCPSEELSPNAFASVVAIVLNGTWHCTHAAAARWIASGSRGSVLNIAATYAWTGSA
                     FVLPSACAKAGVVAMTRSLAVEWGRHGIRVNAIAPGPIPTEGAFSRLMPDPSFVEAHR
                     RKIPAGRFGTPEELAELAAFVVSDAADWLRGEVITLDGGEWLRGAGEFNDLLSLPAET
                     WHALRKRKPKTRNGEDR"
     gene            6530..7489
                     /locus_tag="VD788_02950"
     CDS             6530..7489
                     /locus_tag="VD788_02950"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HXV75249.1"
                     /translation="MNPTNPIALVAMGGHAFMQKNEAGTIEDHERNSDHISELLLTIV
                     ERGYNLIVTHGNGPQVGNLLIQQEQTRDQIPAMPLDVLVAMTEGSLGYVLQQSLLNRL
                     RSLDVKRYVVTVVTQVIVDEDDPAFKEFNKPIGPFLEREEAERRRDEWGWQIRQDPAG
                     RGWRRMVPSPRPQKVIQRHMIRESAERGNIVVAAGGGGIPIRIKPDGNYEGIEAVVDK
                     DLTSSVLACDVRAELLIILTAVPQVFTHFNTPEQSALSAVTVDEIDRLHRAGHFPPGS
                     MGPKIEAAIHFLRHGGRRTLITDPESLPRAFEGRAGTHIIGKL"
     gene            7694..9349
                     /gene="hflX"
                     /locus_tag="VD788_02955"
     CDS             7694..9349
                     /gene="hflX"
                     /locus_tag="VD788_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXV75250.1"
                     /translation="MAAISRRLNRRVGLLIDRAGRIEAVAVGEAHRLTVPRQPSAPSG
                     RLRFCTLRFIATQLDDDDPSAADLASIALHRLDAMAWVAVDDGGQPGPVRVAHLLPAP
                     ARARHNGGGERRVSGGEPLPRGFARPRPARSPRKARRVSQDIEADGERYRLWPPRPAA
                     RIDEDFLDLIRALEEEFDRHLPRVKRAGQVGRAILVHVTTGPRADAEASIDELAELAA
                     SADLEVVERVVQRRAAFDPKTLMGSGKLQDLIIHALRLQADFIVVDQDLTPAQARAIA
                     VATDLKVIDRTQLILDIFADRARTHEGKIQVELAQLNYLLPRLAKRGDSGLSRLEGGI
                     GGRGPGEQKLEIDRRRVRERIRGLERQLLAERKRREQRRSRRRQRDVPVISLVGYTNA
                     GKSTLLNLLTRSDVFVEHRMFATLDPTSRRLRLPREREVIINDTVGFIRDLPRDLIAA
                     FRATLEEIEDSDLIVHLVDISHPDHEAQIEAVRRILEELDYDDIPHLLVFNKADRLDA
                     QELAERVNGRDGVVVSALRGAGIDALLEQIDCALPRSAQVEAR"
     gene            complement(9372..>9992)
                     /locus_tag="VD788_02960"
     CDS             complement(9372..>9992)
                     /locus_tag="VD788_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75251.1"
                     /translation="QLQDLWWSEAARYQVLPLDGRKTVRLSAELQGRPSLAGGRKTFT
                     YYPGVEALPAGSAPSLLNKSWKITAELEIPKGEVQGAVFSMGALDGGYGLYVQKGKPV
                     FVGNFLGRAWTRVTSKSALPAGAVTLRAELAYDGGGLGQGGTMALYVNDAKVGEGRIE
                     ATHGITLGLGGTLDVGMDSGAPVDDAYTPPFRYAGTIRQVTVDLEP"
BASE COUNT         1629 a         3499 c         3420 g         1444 t
ORIGIN      
        1 aagcgagcga ccgagcaacg ccggttgaaa gccgagaacg tccggctccg cgacaatgtc
       61 gaccggctgg tgacgccgag ccagatcgtc ggcgagagcc cgcagatgcg caaggtgctc
      121 gagctcgtcg agtcggtggc cgtgaccgag gcgacggtgc tgattcgcgg ggagagcggg
      181 accggcaagg agctgatcgc acacgcgatc cataccaaca gcagccggcg ctacttcccg
      241 atcatcgcgg tcaactgcgg cgcgctgccc gagtcgctgc tggagagcga gctgttcggt
      301 cacgagaagg gagcgttcac cggcgctcaa ttccgccgca agggcaagct cgagatggcg
      361 gacggcggga cgctgttcct cgacgaggtc ggtacgatca gcctgcgcac gcaggtcgac
      421 ctgctgcgcg tgctggagac gaaggagttc acccggctcg gcggcacgcg cccggtgcgc
      481 gtcgactttc gcgtgatctg tgcgaccaac cagaacctcg agcaccaggt ggaacagggg
      541 cagttccgcg aagacctgta cttccgcttg aacgtggtga cgatccagct gccgccgttg
      601 cgcgaccgcc gggaggacat cccgcagctg gccgagcact ttgccggaaa gtacgcccgg
      661 cagatgaacc gtccgttcga acggatcgac ccggcggcga tggatctgtt ggtgcgctac
      721 gactggccgg gcaacgtcag ggagctggcc aacgcgatcg aacgggcgct ggtcgtcggc
      781 aaatcgagca cgattcgcgc cgacgacctg ccgctccggc tgagtcagcc gtgcaacggt
      841 tcgagcggtg attcgctggc cgacatcgag cgcgagcaca tcgcccggat gctcgaacgc
      901 gagggttgga acatcaccca caccgcgagc aagctgcgca tcgaccgagt cacgctctac
      961 aacaagatca agaagtacgg actgcgcagg tgaacggggc gctgcggtgg tggcgagtcc
     1021 ggcgacagtc gatctggtac cgatctacct gacggactac ccgcgaatga ccgacgcggt
     1081 cggcgtcgcg ctgcgcgaac ggctcggcct gcgcgtccgc ctgcgcaagc cgtggttcga
     1141 tccggaagcg gcctacgacc tcgcgcgcgg ccagtacagc tcgcgcgtgc tgctcgagct
     1201 gctgctggac gaccccgatc cgggttcggc gcgcatcgtc gcgatcacga cgatcgatct
     1261 gttcgccccg gttctgacct acgtcttcgg cgaggcgcag ctcgacggac gtgccgcggt
     1321 ggtctcgctg caccggctgc gccccgaggc ctacggcctg gcgtgcgacg aacggctggt
     1381 gcgcgcgcgc ctggtgaccg agacggttca tgaattgggt cacaccttcg gtctgctgca
     1441 ctgcaacgaa tcgtcgtgcg tgatgcatcc ttcgacctac gccgaagaga tcgatcagaa
     1501 aacggaccgc ttctgcgacc tgtgttcgtc gctgctgtct cgagtttccc cgccggcgat
     1561 ctgttgatac catgctcgct ccggaggacc tggcacgcag acggaacgtc tggaggagca
     1621 agccgatgat cgacagcaag ctggagaagg cgatcaacga tcagatcaac aaagagctgc
     1681 aggcgtggta cagctacctc gcgatgtccg cctactgcaa gaagctcaat ctgagcggct
     1741 tcgcctcctt catggacgga cacgcacggg aggagcaggc gcacgcccac cgcctgatga
     1801 actacgtgct cgaccgcgac ggctcgatcg tgctccaacg gatctcggca ccggcggccg
     1861 acttcaactc gctgctccac ctgttcgaac tggcggtcga gcaggagcgc tcgaacaccc
     1921 ggtcgatcta cgagctgtac gagctggcca agcaggtcaa cgactactcg accgtcgccg
     1981 cgctgcagtg gttcctcgac gagcaggtcg aggaggagaa ggtgatgacc gaggcactgg
     2041 gcctgatgcg cttcgcgggc gacgacaaga gcgcgctgtt gacgctgaac agccagttcg
     2101 gcaagcaggc gacggtcgaa acaccgagcg aatgaccggg tcgccgcgat caatcacggg
     2161 cgagttcccg gaccctgcat acggttctcc agttacgatc cgtcatcggc acaccgagcg
     2221 cctgctccgc cccggccgcg agcttggacc gccccacgcc ttccggcgcg tggaggtaga
     2281 agacccggcc gatgagacgg aaccgctcac tgccttgccg gagacggtcg agcttggcca
     2341 gatccggtct tttcggcagg ctggccagga aacccaggtg caggctcgcg ggagcgctct
     2401 cggcttcggg aaacgggttg cgcatcatcg cctcgtcgat cgcgtcgagc gatagaacct
     2461 gggctcgcgc gtcgaagccg aactcctttt cgatctcggc ggatagcttt ctcgccaacg
     2521 ccgacgagcc ccgcgcggcc acctcgaaca cggcgttgcc actctggatg tacgtcctca
     2581 cgtttcgagc gccgagccgc tcgaggatgc gaaccaagtc cttcatcggc agcttgttcc
     2641 tgccaccgac gttgattcca cgcagcagcg ccacgaacga ttcggctcgg gcaaccaact
     2701 acgcaccccc gatggccgcg caccgaccac ccgccccgcc agactacccg gaagtctcgt
     2761 ttacgatcga gagaatcgcc gtgccgtagt ccttgacccg cttgggtccc atcccccgga
     2821 cccgcaacaa cgacgattcg tcggtcggtc ggctgcacgc gatcgccgtc agatcgcgat
     2881 tcgacagaat gcagaaggcg ggcaggccgc ggcgtctcgc ctcggccagc cgccagtcgc
     2941 gaagcgcctc gacgagcgcc gccggcggcg cctcgatcgc ccgttccgcg gggaccgcag
     3001 tttgcccgga cggcaccgtg cgcgaacgcg tacttcgcgg cgcgcgcgaa ccgccccgat
     3061 cgggggggac cggaatgggc acgtccgccg gcagaccgct cgcgcgaccg cgcgaggtca
     3121 gctcgacgcg gcggaagcgg atcagccggc cgtcccgttc gaacgaatcc tcgcgcacgc
     3181 gaaccacgcc ggcgcgggcc agccccgcga tcagacgctc gcactcgtcg cggctcgctc
     3241 cgtctccgaa cagctcgcga tgcaagcgcc ccgtcggctg gccgtcacgc tcggcgagcg
     3301 aggaaacgat tcgctcgagc gcgcgcgact gaccggcgtc cggcgcggtg aagcgcagca
     3361 cgacgcagga gtccggggca cagaaatcgc agcttccgca cgggccggca ccgtcctcgc
     3421 gctcgccgaa gtgccggacc aggtggagca tgcggcactc gcggctgtcg gcgaagcggg
     3481 tgacgaggtc gagttcgtcg cgcttgtggc ggcgttgggc gtcgtaggcc tgtcgccagc
     3541 cgtcgtcgcc gagacgtgcg ttgccggcgg ggtcgacccg cgcgccgccg tggatccaca
     3601 gtttctcgag cgcgacgtcg aaccgctccg gatcgagccg cgtggcgagc aacagctcct
     3661 cgcgcgagcg ccaaacgtcc gccagctcga tccgcagccg ttcgagctcg cgcgggtccg
     3721 gatacgacct gtcgagaaag tactcgtgcg tgcggcggtc ggcgtagccg tgcagcagca
     3781 cggcgcggga ggggagtccg tcgcggccgg cgcgcccgat ctcctggtag tacgactcga
     3841 cgctgcccgg cagcgcggtg tggacgatcg tgcggacgtc cggtttgtcg atgcccatgc
     3901 cgaaggcgat cgtcgcgacg acgacgtcga gccggccggc gaggaacgcg gcctggacct
     3961 cgtcgcgtcg ggcaccgccg agacccgcgt ggtacggggc cgcgggaaaa ccgacggcca
     4021 gtcgcccggc cagctcctcg gcctgccgtc gcgtcggcgc gtagacgatc gccggacgtc
     4081 gcgcgggatc cgccagcaag cgctcggtca tcgcctggcg cgccgacatc cggagctccg
     4141 ccagctcgat cgcgatgttc gcgcggcgga aaccgtggat gaacaagccg gtctcgcgca
     4201 taccgagctg gcggacgatg tcgcgttgca cgagccgggt cgcggtcgcg gtcagcgcga
     4261 tgacgggggc cggtctcagc gacggcagcc gctcgccgag catgcgatag tccggacgga
     4321 agtcgtgacc ccactgcgag atgcagtgcg cctcatcgac cgcgaccagc gccggcacgc
     4381 gtcgcgccag catctcgggg aaccccgcga cacccagccg ttccggcgcg acgaacagga
     4441 agtcgagcgc cccgtcgagg tagtcgcggc agacgcgctg ggattcgccc cggtcgcgcc
     4501 ccgagtggat ccgctcggcg cgaaacccgc gcgccttcag ccgattcgcc tggtcctcca
     4561 tcaacgcgat cagcggactg acgacgagcg tcgtcccgcc gcgcgcgatg cccggcagct
     4621 ggtagcacag cgacttcccg gcgccggtcg gcatcaccag caacacgtcg cgccccgcgg
     4681 tcacctcgcg gcagacctcg agctggtgcg gccggaagcc ggtgagcccg aaccgttcgc
     4741 gtagcagccg ttcgagcccg tcgacccctg tcgattggcg ctgcgacatc gcccgccagg
     4801 gtagcaccac gagcccagcc ttcagaacgg cagcgcccgc cgtcccggcc cgcgcaaccc
     4861 tccgctgtcc gcgggcgacg cgccgcaggc gttgtcgagc agttgctcga ccgcttcgcg
     4921 gagcccgagc ggtgcagccg agccgcccat ccgtgtgcgg acccagccgg ggtgcaccgc
     4981 gacgcagacg aagccctccg gcctcagctc gtgcgccagg ttgcggttgg ccatgttcaa
     5041 cgccgctttc gacatccggt aggcgtagga gccgcccgag ccgttgtcgc cgatcgagcc
     5101 catcagcgac gtcacctgga cgatccgctt tccgtcgccg cggcgcaaca acggcagcag
     5161 cgcccgcgta actcgcaacg ggccgagcgc gttcacttga aacgccgtga gcagggcgcc
     5221 gagatcgagc gtctcgatgc caccgccgtc ctgcgggtag atgccggcat tgttgatcag
     5281 caggtcgaga cggtcggtca gctcggcgac acggccggcc gcgccggcga ccgactgatc
     5341 gtcggcgacg tccatcacga cctcgtgcac gcgaccggga tgatcgcgcg ccagatcggc
     5401 gatcccggtc gagccggcca cgcgcaccgc ggcgaagacg aggtggcccc ggtcgacgag
     5461 ctggctgacc gcctcgagcc cgatgccccg cgcggcaccg gtcaccagcg cgacttgtcg
     5521 cgtcatcgat cctctccgtt tctcgttttc ggtttgcgct tgcgcagcgc gtgccaggtc
     5581 tcggcgggaa gcgagagcag atcgttgaac tcgcccgcgc cgcgcaacca ctctccaccg
     5641 tcgagcgtga tcacctcgcc acgcagccaa tcggccgcgt cagagacgac gaacgcggcg
     5701 agctcggcga gttcctccgg cgttccgaag cgaccggccg ggatcttccg ccggtgggcc
     5761 tcgacgaagg acgggtcggg catcagccgc gagaacgccc cctcggtcgg gatcggcccg
     5821 ggcgcgatgg cgttgacgcg aatcccgtga cgcccccact cgacggcgag cgagcgcgtc
     5881 atcgcgacca cgcccgcctt ggcgcaggcc gacggcagca cgaacgccga cccggtccag
     5941 gcgtaggtcg ccgcgatgtt gagcaccgag ccgcggctgc cgctcgcgat ccagcgcgcg
     6001 gcggcggcgt gcgtgcagtg ccaggttccg ttcaacacga tcgcgaccac cgaggcgaac
     6061 gcgttgggcg acagctcctc cgaagggcag aggaaattcc ccgcggcatt gttgaccaac
     6121 acgtcggggg cgccgagcgc ctgcgtgacg cggtcgagcg ccgcgccgac gcccgtcggg
     6181 tcgcgcacgt cgcagacggc ggccagcgcc cggccgcccg cggagacgat ctgtcgcgcg
     6241 gtctcctcga gcggttcggt gcgccgtccc gacaggccga cggcggcgcc gagaccggcg
     6301 aatcgcagcg ccatcgcccg cccgaggccg gtgccgccgc cggtgatcca ggccgttttt
     6361 ccggcgagca gccccggagc cagacacgcg ccgtcggttc tcatcgctgg tctcccgtga
     6421 acaggcgacc ctatcttgat ctaccgggcg cacggtttca accacgcgcg ttgccggccg
     6481 cggccgtcgc gtgcgacact cgtgcggtcg aaacgggaga ccgatgtcga tgaacccgac
     6541 caacccgatc gcgctcgtcg ccatgggcgg tcacgccttc atgcaaaaga acgaagcggg
     6601 cacgatcgaa gaccacgagc gcaactccga tcacatcagc gagctgctgc tgacgatcgt
     6661 cgagcggggc tacaacctga tcgtgaccca cggcaacggg ccacaggtcg gtaacctgct
     6721 gatccagcag gagcagaccc gtgatcagat tccggcgatg ccgctcgacg tcttggtggc
     6781 gatgaccgag ggcagcctgg gctacgtgct gcagcagagc ctgctgaacc gtctgcggtc
     6841 gctcgatgtc aagcgttacg tggtgacggt cgtgacgcag gtgatcgtcg acgaggacga
     6901 ccccgcgttc aaggagttca acaagccgat cgggccgttc ctcgagcgtg aggaggccga
     6961 gcgccgccgt gacgagtggg gctggcagat tcgccaggac ccggccggcc gcggctggcg
     7021 gcggatggtg ccctcgccgc ggccgcagaa ggtgattcag cgccacatga ttcgcgaatc
     7081 ggccgagcgc ggcaatatcg tcgtcgccgc cggcggcggc ggcatcccga tccgcatcaa
     7141 acccgacgga aactacgagg gcatcgaggc ggtcgtcgac aaggacctga cgtcgagcgt
     7201 gctggcctgc gacgtgcgcg cggagctgtt gatcatcctg accgcggtgc cgcaggtttt
     7261 cacgcacttc aacacgcccg agcaaagtgc gctgtcggcc gtgacggtcg acgagatcga
     7321 ccggttgcac cgggcgggcc attttccgcc gggcagcatg ggacccaaga tcgaagcggc
     7381 gatccacttc ctgcgtcacg gcggcaggcg aacgttgatc accgatcccg agagcctgcc
     7441 gcgcgccttc gaggggcgcg ccggcactca catcatcggc aagctgtagc tcatgacgaa
     7501 ccgctcgacg cgcagtacat taggggtgtg aactgcggcc cgaccggcgg acggcgggcc
     7561 gggaggaact tcgataccga gcctgcatgg aaatctcctg ggtctgagcc cggcggaaaa
     7621 gcagagtctc gagcgcttgt accgccggcg cgtcccggcg acctcgatcc ttacccacga
     7681 actggcacgc gagatggcgg cgatcagccg ccggctgaac cgtcgtgtcg ggctgttgat
     7741 cgaccgcgct ggtcgcatcg aggcggtcgc ggtgggcgag gcgcatcgcc tgaccgtgcc
     7801 gcgccagccg agcgcgccct ccggccggct acgcttctgc acgctccggt tcatcgccac
     7861 ccagctcgat gatgacgacc cgtcggccgc cgacctggcc tcgatcgcgc tccatcgact
     7921 cgacgcgatg gcgtgggtcg cggtcgacga cggcggtcag ccggggccgg tgcgtgtggc
     7981 gcacctcttg cccgcgccgg cgcgcgcgcg gcacaacggc ggaggcgagc ggcgtgtgtc
     8041 cggcggcgag ccgctgccgc ggggcttcgc ccggccgcgt cccgcgcgct ccccgcgcaa
     8101 ggcgcggcgg gtgtcgcagg acatcgaggc cgacggcgag cgctaccggt tgtggccgcc
     8161 gcgaccggcg gcgcggatcg acgaggactt cctcgacctg atccgcgcgc tcgaggaaga
     8221 gttcgaccgg catctgccac gcgtcaaacg cgcgggtcag gtcggccgcg cgatcctggt
     8281 ccacgtgacg accggcccgc gcgcggacgc cgaggcatcg atcgacgagc tggccgagct
     8341 ggcggcatcg gccgatctcg aggtggtcga gcgcgtcgtg caacgacgag ccgcgttcga
     8401 tccgaagacg ctgatggggt cgggcaaact gcaggacctg atcatccacg cgctgcggct
     8461 gcaggccgac ttcatcgtgg tcgatcaaga cctgacgccg gcgcaggcgc gcgcgatcgc
     8521 ggtcgcgacc gacctcaagg tgatcgatcg cacgcagttg atcctggaca tcttcgccga
     8581 tcgcgcgcgt acgcacgagg gcaagatcca ggtcgagctg gcccagctca actacctgct
     8641 gccacgactg gcgaagcggg gggattccgg tctgtcgcgg ctcgagggcg ggatcggcgg
     8701 ccgcggcccc ggcgagcaga agctcgagat cgaccggcgc cgggtgcgcg agcggatccg
     8761 cggtctcgag cgacagttgc tcgccgaacg caagcggcgc gaacagcgcc gttcgcgccg
     8821 gcggcagcgc gacgtgccgg tgatctcgct ggtcggctac accaacgccg gtaagagcac
     8881 gctgctcaac ctgctgacgc gctccgacgt cttcgtcgag caccgcatgt tcgccacgct
     8941 cgacccgacc agccgtcgcc tgcgattgcc gcgcgagcgc gaggtgatca tcaacgacac
     9001 cgtcggtttc atccgcgacc tgccgcgcga cctgatcgcg gccttccggg cgacgctcga
     9061 ggagatcgaa gactcggact tgatcgtgca cctggtcgac atctcgcacc cggaccacga
     9121 ggcgcagatc gaggccgtgc gacgcatcct cgaagagctc gactacgacg acatccccca
     9181 cctgctggtg ttcaacaagg ccgaccggct cgacgcgcag gagctcgccg agcgggtcaa
     9241 cgggcgggac ggggtcgtgg tctcggccct gcgcggcgcg gggatcgacg ccctgctcga
     9301 gcagatcgat tgcgcgctgc cgcggtcggc gcaggtcgag gcccgctgat ccggtctcgt
     9361 tccccggtcg gctacggttc gaggtcgacc gtcacctggc ggatcgtccc cgcgtagcgg
     9421 aacggcggcg tgtaggcgtc gtccaccggg gcgccggagt ccatgccgac gtcgagcgta
     9481 ccgcccaggc cgagggtgat gccgtgggtc gcctcgatgc ggccctcacc gaccttggca
     9541 tcgttgacgt agagcgccat cgtgccgccc tgccccaggc cgccgccgtc gtacgccagc
     9601 tccgcccgca gcgtgacggc gccggcgggc aacgcgctct tcgacgtcac ccgggtccag
     9661 gcgcggccga ggaagttgcc gacgaacacc ggcttgccct tctgcacgta gagcccgtag
     9721 ccaccgtcga gcgcacccat cgagaacacc gccccctgga cttcgccctt ggggatctcg
     9781 agctcggcgg tgatcttcca ggacttgttg agcaagctcg gcgcgctccc cgccggcaac
     9841 gcctccaccc ccgggtagta ggtgaacgtc ttgcgcccgc cggcgaggct cggccggccc
     9901 tgcagctcgg ccgacagccg caccgtcttg cggccgtcga gcggcagcac ctgatagcgg
     9961 gcggcctcgg accaccacag gtcctggagc tg
//
LOCUS       DASPGN010000105         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_369702, whole genome shotgun sequence.
ACCESSION   DASPGN010000105 DASPGN010000000
VERSION     DASPGN010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369702"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VD788_02965"
     CDS             <1..1380
                     /locus_tag="VD788_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75252.1"
                     /translation="TTNRNMRRLAMTLGQLVDLAPPRVLIYFADVTRSNPGEHYLSFF
                     GSNLVHSQPALEAISADAVAGGLAFDQVVNQASAQGVRIYTVEARGLVADADRARTGS
                     ATAAATGQNPPSSSRVRIGDTHRTLQGMAAETGGYAFLHGVRASKIAERIREDSQCVY
                     LASFDPGRFARDQPLRLVVELVGRPEVELRVRGRIVIESASARKTAELLRAFAAPNSI
                     PDPFEVNAGLVPTGFDHGRYTALLQVAVPGSPLPTATWDLGASLVMNDKVREKAAGRL
                     SANGPNVPVILESQVSFKPGAYELVAVAHEEHSGLVSSTELFVDWPSLDEPETMVGPI
                     ALLQPATGAFMRQGETRGEGSLLVAVDRPLSTTLPSAFVGIVCRRKADRRLEIRRAVI
                     GSATHEFPVLELGGEAEHCAQLRDVVPSNTLPRGYYSYTIEVLADGATLDRRQRDFVV
                     IVGEPAGPD"
     gene            1481..>2014
                     /locus_tag="VD788_02970"
     CDS             1481..>2014
                     /locus_tag="VD788_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75253.1"
                     /translation="MDTGRQVLCRLALAVVLSFGVVVGATDERPPGADDDAPRDLGLV
                     ERAGRGLVQLDLSVRGSWEAISNLGAQDFRVSVNGNPIEQLTVDRLCGPPAAAETATG
                     GGTEGTPAAHPSPGASYLFYFDQPMLTAAGRQNSIDIARRMLPELIGDDDRAAIVSAG
                     KRLETLVELTGDTEALLA"
BASE COUNT          299 a          688 c          718 g          309 t
ORIGIN      
        1 acgaccaatc gaaacatgag acggctggcg atgacgctcg gacagctcgt cgacctggcg
       61 ccgcccaggg tgctgatcta cttcgccgac gtcacgcggt cgaaccccgg tgaacactac
      121 ctcagctttt tcggctcgaa cctggtccac tcgcaaccgg cgctcgaagc gatctccgcc
      181 gacgccgtcg ccggcgggtt ggccttcgat caggtggtga accaggcgtc ggcgcaaggc
      241 gtacggatct acaccgtcga ggcgcgtggc ttggtcgccg acgcggatcg cgctcgcacc
      301 ggctccgcga ccgccgcggc gaccggtcag aacccgccga gttcctcgcg cgtccggatc
      361 ggcgacaccc accgcacgct gcagggcatg gcggcggaga ccggcggcta cgccttcctg
      421 cacggcgtgc gggcgtcgaa gatcgccgag cgaatccggg aggactcgca gtgcgtctac
      481 ctggcgagct tcgacccggg aaggtttgcc cgggaccagc ccttgcggct ggtggtggag
      541 ctcgtcggcc ggcccgaggt cgagctgcgc gttcgcggtc ggatcgtgat cgagagcgcg
      601 agcgcgcgaa agacggcgga gctgctgcgc gccttcgccg cgccgaactc gatccccgac
      661 ccgttcgagg tcaacgccgg cttggtcccg accggcttcg accacggcag gtacacggcg
      721 ctgttgcagg tcgccgttcc gggctcaccg ctgccgacgg cgacctggga cctcggcgcg
      781 tcgctggtga tgaacgacaa ggtgcgcgag aaggccgcgg gccgattgtc ggcaaacggg
      841 ccgaacgtcc cggtgatcct cgagagtcaa gtctcgttca agccgggtgc gtacgagctg
      901 gtcgccgtcg cgcacgagga gcacagcggg ttggtctcgt cgacggagct gttcgtcgat
      961 tggccgtcgc tcgacgaacc cgagacgatg gtcgggccga tcgccctgct gcaaccggcg
     1021 accggcgcct tcatgcgcca gggcgagacc cgcggcgaag gatcgctgct ggtcgcggtc
     1081 gaccgaccgc tgtcgacgac gttgccgtcg gcgttcgtcg gcatcgtctg tcgccgcaag
     1141 gccgatcgac ggctcgagat ccggcgcgcg gtgatcgggt ccgccacgca cgagtttccg
     1201 gtgctcgagc tcggcggcga agcggagcac tgcgcgcagt tgcgcgacgt cgtaccgtcg
     1261 aacaccttgc cgaggggcta ctacagctac acgatcgagg tgctcgcgga cggggcgacg
     1321 ctcgaccggc gccagcgtga cttcgtcgtg atcgtgggcg aaccggccgg gcccgactga
     1381 tgtcgttgcg cgcgcgcaac tggacgagtc gatccgcacc cccgccgttt gaccgatctc
     1441 cgcgatcggc ggatgatgtc cactcgtagt ggagggacga ttggataccg gacgtcaggt
     1501 cctttgccgg ctcgccttgg cggtcgtgct gtcgttcgga gtcgtcgtgg gcgcgaccga
     1561 cgagcgaccg ccgggcgcgg acgacgacgc gccgcgcgat ctcgggctgg tcgaacgggc
     1621 agggcgcggg ttggtccagc tcgatctgtc ggtgcgcgga tcgtgggagg cgatctcgaa
     1681 cctcggcgcg caagacttcc gcgtttcggt caacggcaat ccgatcgagc agctgacggt
     1741 cgaccgcctg tgcggtccgc cggcggcggc ggagaccgcc accggcggcg ggacggaggg
     1801 cacgcccgcc gcgcacccca gccccggggc atcgtacctg ttctacttcg accaaccgat
     1861 gctcacggcg gccggtcgtc agaactcgat cgacatcgcc cgccgcatgc tacccgagct
     1921 gatcggcgac gacgaccggg cggcgatcgt ctccgccggc aagcggctcg agacgctcgt
     1981 cgagctgacc ggcgacaccg aagcgctgct cgcc
//
LOCUS       DASPGN010000106         6500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_253832, whole genome shotgun sequence.
ACCESSION   DASPGN010000106 DASPGN010000000
VERSION     DASPGN010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253832"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..754)
                     /locus_tag="VD788_02975"
     CDS             complement(122..754)
                     /locus_tag="VD788_02975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HXV75254.1"
                     /translation="MEIVDPSVERYLHRLADSPDPILREMEALAAERRFPIVGPAVGR
                     LLALLARMIRARRVIELGSGFGYSAHWFARAVGAEGLVVLTDRDGEHAAEAAEFLRRA
                     GLLERTRIETGNALQVIDRVDGEFDIVFNDIDKRDYPRVLDKAAAVLRPGGLLISDNM
                     LWRGAVLDAETADPATRGVRDLTDALYASDRFETVLLPLRDGVTVSSFRG"
     gene            813..1409
                     /locus_tag="VD788_02980"
     CDS             813..1409
                     /locus_tag="VD788_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXV75255.1"
                     /translation="MTETSAGLPSRDDAWRLLNQYTGKPGLLAHGLAVEAALRAYAAR
                     FDGDPAAWGIVGLLHDFDYERWPTADDHPYRGAEILQQLGYPSWFRRAILSHADYSGV
                     TRVSRLEHALYACDELSGFLTACALVRPDRSIRTLTVESVRKKMKSKAFAAGVNRDDI
                     IRGAAALGVELDAHIEFVRGAMSEIADRLGLDGGGGLY"
     gene            1434..1727
                     /locus_tag="VD788_02985"
     CDS             1434..1727
                     /locus_tag="VD788_02985"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HXV75256.1"
                     /translation="MMARPGRLAEAELRARIGELPGWRLEQGKLRRSFQFSDFVQAFR
                     FISGVALIAERMDHHPEWSNVYNRVEMALTTHDAGGVTELDLALAREASRLAG"
     gene            1734..2429
                     /locus_tag="VD788_02990"
     CDS             1734..2429
                     /locus_tag="VD788_02990"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXV75257.1"
                     /translation="MLRLVIFDCDGVLVDSEPISTRVFAEELNRAGVDWPASRVAVEL
                     TGLSDRSCIELVERTTGRRLPAGFLARVRDRTYSAFRAGLRPIDGIEGALERIGLPSC
                     IASSGDHERMRLTLGLTGLLDRFRGRIFSAEDVARGKPFPDLFLHSARALGVDPREAV
                     VVEDSMPGVRAARAAGMTVLGFAAAADGARLARDGARVFGDMRELPELIESVRTASAN
                     RVGEDESAQLSSD"
     gene            complement(2411..2854)
                     /locus_tag="VD788_02995"
     CDS             complement(2411..2854)
                     /locus_tag="VD788_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HXV75258.1"
                     /translation="MNPSGETLSDRMRANGLRMTSQRTILAELLESASEHLDAEKVYQ
                     LARRRDPKIHRATVYRTLNTLKKLGLIDELDLMHVTGDRHYYEIRPSVFHIHLVCMKC
                     GAVEEPNDPFWQDLKKRVVERTGFRPEIVRLEMGGICTACQSDDS"
     gene            complement(2940..4361)
                     /locus_tag="VD788_03000"
     CDS             complement(2940..4361)
                     /locus_tag="VD788_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide excision repair endonuclease"
                     /protein_id="HXV75259.1"
                     /translation="MSRPRRTVSLVAERALRYLRASDGPATSVELAGEVLATRAVDES
                     TAKRVLDAAFAGDPRLVYRRGRWSAESRPAPRPARRPAGERPDEPDRVLLIVEGELSR
                     TNPRFALRAVSAVRMRDELVVGACGGHATDRGSSEQLRRSVLETIEGAIPVVHDPPGA
                     LDALERWLGEPLPAVVSLRELARRRLGLRKHADLETLVARLGLVWYGRNEPVDLAETL
                     EECLARLRRPDEDLRQIQRELSGEVGRLDWSRFAFDRAFIDAVPAVPGTYRFYDRRDE
                     LLYVGKSRNLARRLTSYFRDGERVVRNRKLLAELYRIEYEPAGSELEAVLREAEQIRD
                     DQPKSNVQREVRERHGRAARLRSILILEPAESPNVLRAFLIRDARLVGRVGIGPRGGG
                     LKRIERILEDYFFSAPAGPTVVAGPDLDVEIVARWLAGNRDRAVAFDPTDLRTSGEVI
                     ERLQWFLGQARPFDTDGTPFRPR"
     gene            complement(4434..5516)
                     /locus_tag="VD788_03005"
     CDS             complement(4434..5516)
                     /locus_tag="VD788_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75260.1"
                     /translation="MQRMISFHPVDLRFFDETIGPLIAGGKVNPQPFVDAAMRFRQSE
                     WHARRYRKRLEQLLATLEPPPPPSEGSVWTKLRSRLEQFDFKADPRARRLEGKVEPEL
                     HLWGRPFLILESSAERVSASIEDYLDAEGDASLDALVVQQLLRIDPALPAEIEPAQIR
                     ELDAELSYRNELLETLKSIHSLARTARGGEQGVALAAPGELALEQLCREVPWRAVWLH
                     SRAVPFWVARDVDGLETVCHSAGVTPPDVLTPCWRLFADAIESFPELRDSLNAELRYD
                     KDLGGFVAPADVPELSAFLNREGARIIQAATREGVGPLCASMLRKIRECAHHAQRSEL
                     GYLEASGIMPVAYDREEDDAETAGVD"
     gene            complement(5524..>6500)
                     /locus_tag="VD788_03010"
     CDS             complement(5524..>6500)
                     /locus_tag="VD788_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV75261.1"
                     /translation="DDVMRLEHAPDSLIVLGAGGVGLELSQFFARIGTRVLLVNRSPL
                     LSRFDVECGRELERALCDETNLELAIPGRILDVEPSPGGLSATIRCGDRQWRQQASAL
                     LMATGRRAAIDELGLEHVGVRTTAAGVVHDDAMRTTNPSIFVAGDATGRHQLLHLANQ
                     EGTVAGHNAAVGQAALRMDRRLKMSVIYTDPPFAIVGANESEIRANVEFVTGRARFAD
                     TGRAITMGARHGLWKLWANKAEGEILGSAIVGPRADDLIHLISTAMYYRATSTALCSM
                     PWYHPTLSEVILNLARDISRQLRVDCTIPGAATLAPGYSTTMRDRAND"
BASE COUNT         1011 a         2242 c         2230 g         1017 t
ORIGIN      
        1 gaggcggtcc atccgccgcc ggtcgtcgcg cgatgtgaac ttcgggtact cgcgtgcggc
       61 cgacgtgatc ttgccgtcgg ccgtcatcgc catgttgaca tagacgaacg gtcggcgcat
      121 ctcagccacg gaaagacgat acggtgacgc cgtcccgcaa cggcaacagc accgtctcga
      181 accgatcgga cgcgtagagc gcgtcggtca aatcgcgaac gccgcgcgtc gcgggatcgg
      241 ccgtttcggc gtcgagcacg gcgccgcgcc acaacatgtt gtccgagatc agcaggcctc
      301 ccggtcgcag gactgcggcg gccttgtcga gcacgcgcgg gtagtcgcgc ttgtcgatgt
      361 cgttgaagac gatgtcgaac tcgccgtcga cgcggtcgat cacctgcaac gcgttgccgg
      421 tctcgatccg ggtccgttcg agcaagcccg cgcgtcggag aaactcggct gcttccgccg
      481 cgtgctcgcc gtcgcggtcg gtcagcacga ccaggccctc cgcgccgaca gcccgggcaa
      541 accagtgggc gctgtagccg aagcccgagc cgagctcgat cacgcgccgc gcgcggatca
      601 tccgcgccag cagcgcgagc aggcggccga ccgcgggccc gacgatcgga aaccggcgct
      661 cggcggccag cgcctccatc tcgcgcaaga tcgggtccgg cgaatcggcc agccggtgca
      721 gataccgctc gaccgacggg tccacgattt ccatgaagac acgctaacat ggtgtctcgc
      781 gcgatgacaa tcgaccgccg ccgggagacg agatgaccga aaccagcgcc gggcttccct
      841 cgcgtgacga cgcgtggcgc ctgctcaacc agtacaccgg caaacccggc ctgctcgcgc
      901 acgggctggc ggtcgaggcg gcgctgcgag cctacgccgc tcgtttcgac ggcgaccccg
      961 ctgcgtgggg catcgtcggt ctgctgcacg atttcgacta cgaacgatgg ccgacggccg
     1021 acgatcatcc ctatcgcggg gcggagatcc tccagcagct cggctacccg tcgtggttcc
     1081 gccgagcgat cctgtcgcat gccgactact ccggggtgac gcgggtgagc cggttggagc
     1141 acgcgttgta cgcctgtgac gagctcagcg ggttcttgac cgcgtgcgcg ctggtgcgtc
     1201 ccgaccggtc gatccgcacg ctgaccgtcg agtcggtgcg caagaagatg aagtcgaagg
     1261 cgttcgccgc cggcgtcaat cgcgacgaca tcatccgcgg ggcagccgcg ctcggtgtcg
     1321 agctcgacgc gcacatcgag ttcgtccgcg gtgcgatgag cgagatcgcc gaccgactcg
     1381 gtctcgacgg cggcggcggg ctatactgaa cgcccgagca agagggagac ccgatgatgg
     1441 cacgacccgg aagactggcg gaagccgagt tgcgcgcgag gatcggcgag ctgcccggct
     1501 ggcggctgga acaggggaag ctgcgcagga gctttcagtt ctccgatttc gtccaggcgt
     1561 tccgcttcat cagcggcgtt gcgctgatcg ccgagcgcat ggaccaccac ccggagtggt
     1621 cgaacgtcta caaccgggtc gaaatggcgc tgaccacgca cgacgcgggc ggcgtcaccg
     1681 agctcgatct ggcgctcgcc cgcgaggcat cccgcctcgc cggttgacgc gggatgctgc
     1741 ggctcgtcat cttcgactgc gacggagtgc tggtcgacag cgagccgatc tcgacgcgcg
     1801 tcttcgccga ggagctgaac cgggccggcg tcgattggcc ggcgtcgcgg gtcgccgtcg
     1861 agctgaccgg gctgtcggat cgttcctgta tcgagttggt cgagcgaacg accgggcggc
     1921 gcctgccggc cggcttcctc gcgcgcgtgc gcgaccggac gtactccgcc ttccgcgccg
     1981 ggctgcgacc gatcgacgga atcgagggcg cgctggagcg catcgggctg ccgtcctgca
     2041 tcgcgtcgag cggcgatcac gagcggatgc ggttgacgct cggtctgacc ggcctgctcg
     2101 atcgcttccg cggacggatc ttcagcgccg aggacgtggc ccgcgggaag ccctttcccg
     2161 acctgttcct gcacagcgcc cgtgcgctgg gggtcgaccc gcgcgaggcg gtcgtcgtcg
     2221 aagacagcat gccgggagtg cgcgcggcgc gtgccgccgg catgacggtc ctgggcttcg
     2281 ccgccgcggc cgacggcgcg aggctggccc gcgacggagc gcgcgtcttc ggcgacatgc
     2341 gcgagctccc cgagctgatc gagtccgtgc ggaccgcgag cgcgaaccgg gtcggggagg
     2401 acgagtcggc tcagctgtcg tccgactgac aggcggtgca gatgccgccc atctcgagcc
     2461 ggacgatttc cggtcgaaag cccgttcgtt cgacgacacg ctttttgagg tcctgccaga
     2521 agggatcgtt cggttcctcg accgcgccgc acttcatgca caccaggtgg atatggaaga
     2581 cggaaggccg gatctcgtaa tagtgccgat cgccggtgac gtgcatcagg tccaactcgt
     2641 cgatcagccc gagcttcttc agcgtgttga gcgttcggta gacggtcgcg cgatggatct
     2701 tcgggtcgcg gcgtcgtgcc agctggtaga ccttttccgc gtcgaggtgc tcactcgccg
     2761 attcgagcag ctcggcgaga atcgtccgct gcgacgtcat gcgcaacccg ttggcccgca
     2821 tccggtcgct cagagtttcg ccgctggggt tcatcgcgcg tcgatccgtc gaggctcgcc
     2881 agtactgtag gaaagcgcgt cggcactgtc aagatcaagg cgacgcgtcg gggtccggtc
     2941 taacgcggtc tgaacggcgt accgtcggtg tcgaacgggc gcgcttgacc gagaaaccac
     3001 tgcagccgct cgatcacttc gcccgaggtg cgcaggtcgg tcgggtcgaa cgccaccgcc
     3061 cgatcgcgat tgccggccag ccagcgcgcg acgatctcga cgtcgagatc ggggcccgcc
     3121 acgacggtcg gtccggcggg ggcggagaaa aagtagtctt cgaggatccg ttctatccgt
     3181 ttgagtccgc cgccgcgcgg gccgatgccg acgcgaccga cgagccgtgc gtcgcgaatc
     3241 aggaaggcgc gcaggacgtt gggtgactcg gccggttcga ggatcaggat cgagcgcagc
     3301 cgagcggccc ggccgtgccg ctcgcgcacc tcccgttgaa cgttcgactt cggctggtcg
     3361 tcgcggatct gttcggcctc gcgcaggacc gcctccagct cggaccccgc cggttcatac
     3421 tcgatccggt agagctcggc gagcaacttc cggttgcgaa cgacgcgctc gccgtcacga
     3481 aagtacgagg tcagtcggcg ggccaggttc ctcgacttgc cgacgtacag cagctcgtct
     3541 cggcgatcgt agaagcgata ggtccccggc acggccggga ccgcgtcgat gaaggcgcga
     3601 tcgaaagcga atcgggacca gtccaaccga ccgacttcgc ccgacagctc gcgctggatc
     3661 tggcgcaggt cctcgtccgg tctgcgcagc cgggccagac actcttcgag cgtctcggcc
     3721 aggtcgaccg gttcgttgcg accgtaccag accagaccca gccgcgcgac cagggtttcc
     3781 agatcggcgt gcttgcgcag gccgagccgt cggcgcgcga gctcgcgtag cgagacgacg
     3841 gccggcaacg gttcgccgag ccagcgttcg agcgcgtcga gcgctccggg ggggtcgtgc
     3901 accaccggga tcgccccctc gatcgtctcc agcaccgatc gccgcagttg ttcgctcgac
     3961 ccgcggtccg tcgcgtgacc gccgcaggcg ccgacgacca gctcgtcgcg catgcggacc
     4021 gcgctcaccg cacgcagggc gaatcgcggg ttcgtgcggg agagctcgcc ctcgacgatc
     4081 aacagcaccc ggtccggttc gtcgggtctt tcgccggcgg gtcgtcgtgc cgggcgggga
     4141 gccggccggc tctcggcgct ccatcgaccc cggcgataca cgagacgcgg atcaccggcg
     4201 aacgcggcgt ccagcacgcg cttcgcggtg ctttcgtcga cggcacgagt ggccagcact
     4261 tcgccggcca gctcgacgct ggtcgccggt ccgtccgacg cgcggaggta ccgcagcgca
     4321 cgctccgcga cgagcgacac cgtgcgtcgc ggacggctca cggccttcgc ctcggttccg
     4381 acatgaccgg ctcgattgtc tcaccgggcg cggtccgagt caaaacgcgc agctcagtcg
     4441 acgccggccg tttccgcgtc gtcctcttcg cgatcgtatg cgaccggcat gatgccgctg
     4501 gcctcgaggt agccgagctc cgatctctgc gcgtggtgcg cacactcgcg gatcttgcgc
     4561 agcatcgacg cgcacagcgg gccgacgcct tcacgcgtcg ccgcttggat gatgcgcgcg
     4621 ccttcacggt tgagaaaggc cgacagctcc ggaacatccg ccggcgcgac gaaccccccc
     4681 aggtccttgt cgtagcgcaa ctcggcgttc aacgagtcgc gcagctcggg gaacgactcg
     4741 atcgcatcgg cgaacaggcg ccagcacggc gtcagcacgt cgggcggcgt cacacccgcc
     4801 gagtggcaga cggtctccag cccgtcgacg tcgcgtgcga cccagaacgg gaccgctcgg
     4861 ctgtgcagcc acaccgctcg ccacggaacc tcgcggcaca gctgctcgag cgccaactcg
     4921 ccgggtgcgg ccagcgcgac gccctgctcc ccgccgcgcg ccgtccgcgc cagcgagtgg
     4981 atcgacttca gcgtctcgag caactcgttg cggtagctca actcggcgtc gagctcgcgg
     5041 atctgtgccg gttcgatctc ggccggcagc gccggatcga tccgcagcaa ctgctggacg
     5101 accagcgcgt cgagcgacgc gtcgccctcc gcgtcgaggt aatcctcgat cgacgccgac
     5161 acgcgttcgg cggagctctc gagaatcagg aacggacgcc cccagagatg cagctccggc
     5221 tcgaccttgc cctcgagacg ccgcgcgcgg ggatcggcct tgaaatcgaa ctgctcgagc
     5281 cgcgaacgca gcttggtcca caccgatccc tcgctcggcg gcggcggagg ttcgagcgta
     5341 gccagcagct gctcgagccg cttgcggtag cgtctggcgt gccactccga ttgtcggaag
     5401 cgcatcgccg cgtcgacgaa cggctgtggg ttgaccttcc ccccggcgat cagcgggccg
     5461 atcgtctcgt cgaagaagcg caggtcgacg ggatgaaacg agatcatccg ctgcatcgtg
     5521 acctcaatcg ttagctcggt cgcgcatcgt ggtcgaataa ccgggcgcta gcgtcgccgc
     5581 gcccgggatc gtgcaatcga ctcgtaactg gcgcgagata tcccgggcca ggttgagaat
     5641 cacctcggac agggtcggat ggtaccaggg catcgagcac agagcggtgg acgtcgcgcg
     5701 gtagtacatc gcggtggaga tcaggtggat cagatcgtcg gcgcgtggtc cgacgatcgc
     5761 cgagccgagg atctcgccct cggccttgtt cgcccacagc ttccacaggc cgtgacgcgc
     5821 gcccatcgtg atcgcgcgtc cggtgtcggc gaaccgtgcg cggccagtga cgaactcgac
     5881 gttcgcccgg atctcgctct cgtttgcgcc gacgatggcg aacggcggat cggtgtagat
     5941 caccgacatc ttcagccgac ggtccatgcg caacgcggcc tggccgaccg cggcgttgtg
     6001 accggcgacg gttccctctt ggttggccag gtgcaacagc tgatggcggc cggtggcgtc
     6061 gccggcgacg aagatcgacg ggttggtcgt gcgcatcgcg tcgtcgtgca cgaccccagc
     6121 ggccgtcgtc cgcacgccga cgtgctcgag acccagctcg tcgatcgccg cgcgacggcc
     6181 ggtggccatc agcaacgcgg aggcctgctg tcgccactgt ctgtcgccgc agcgtatcgt
     6241 cgccgacagc ccgccgggcg acggctcgac gtcgaggatc cgtcccggta tcgccagctc
     6301 gaggttggtc tcgtcgcaca gcgcgcgctc cagctcgcga ccgcactcga cgtcgaaacg
     6361 cgacagcagc ggggagcggt tgaccagcag gacgcgcgtg ccgatcctcg cgaagaactg
     6421 cgacagctcg agcccgacgc caccggcccc cagcacgatc aacgagtcgg gggcatgctc
     6481 gagccgcatc acgtcgtcgc
//
LOCUS       DASPGN010000107         3870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_136056, whole genome shotgun sequence.
ACCESSION   DASPGN010000107 DASPGN010000000
VERSION     DASPGN010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136056"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VD788_03015"
     CDS             <1..647
                     /locus_tag="VD788_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXV75262.1"
                     /translation="AVFSPRMTSESLFAWSALLQAAGGAIVAVARVERGADDELLIRR
                     DRGANSTGARWVLGEVGTPDAVLEAVGAGRVDTLIVFGDPLDPDETPTVPDQARAALT
                     ELVYVGAFADAVASRAGVVLPVAAWAEEDGTIVNFEGRIQRVRRAHLPRGERRPAWRV
                     AVDLARAAEIDLPQWSSAADVLASLGTAVAAYDGLDEETIGLLGIAATAAAAGA"
     gene            662..1711
                     /gene="nuoH"
                     /locus_tag="VD788_03020"
     CDS             662..1711
                     /gene="nuoH"
                     /locus_tag="VD788_03020"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HXV75263.1"
                     /translation="MFDWTTLLIAAIEILVVVNVLMVGVAGMTWVERRLSAVIQFRWG
                     PNRVGPAGLLQPIADGIKFLWKEEMLPRDAYMPVFILAPALAMVTSIFAFSVIPFGPT
                     IRLFGRSIDLVLLDMNGGILFAFAATSIGVYAIVCAGWASRSKYALIGGLRSSAQMIS
                     YELTLGLSVIGVIIASGTLRPTVIVEQQAGWFWSWNMLGGGQLLGFLLFVIAGFAETN
                     RLPFDLPEAESELVAGYHTEYSSMKFSMFFMAEYIAMITMSALATTLFLGGYNPGFPP
                     EAIGLSDGWPLWILQLFSFVVKVGLFMFFFVWVRWTLPRFRYDQLMDLGWKRLFPLAL
                     FNIFLTGFLVAFDVI"
     gene            1722..2225
                     /locus_tag="VD788_03025"
     CDS             1722..2225
                     /locus_tag="VD788_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXV75264.1"
                     /translation="MSLELVSFVVLTALALITGLTVIRHRNQVVAALALAGNLVSIAG
                     FYMLLNAQFLALLQVIVYAGAIMVLILFVLMLLNLPDEARRKRDGWLQSTVGLLLAVA
                     FVAIVGRAIAESTAADGFPVNPAGFGTVESVGTSLFSDYFYAFEAISLLLVVAMVGAV
                     LLAKRRL"
     gene            2228..2527
                     /gene="nuoK"
                     /locus_tag="VD788_03030"
     CDS             2228..2527
                     /gene="nuoK"
                     /locus_tag="VD788_03030"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HXV75265.1"
                     /translation="MTPQMVLVVSAALFAIGVVGVLSRRGAITILMSIEIMLNAGNLA
                     FIAFSRMQDQPDGQVYAFFVMTLAAAEAAVGLAIVIALFRLRESTEVDELNLMKW"
     gene            2535..>3870
                     /gene="nuoL"
                     /locus_tag="VD788_03035"
     CDS             2535..>3870
                     /gene="nuoL"
                     /locus_tag="VD788_03035"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HXV75266.1"
                     /translation="MLDLIWLIPLLPAAGVLINGFWGRRLSRPAVGAVGSATVGLALL
                     LAVGAVWQLSGLDPALRVHEVSLGTWMPLGTVGGAELVIDWGFRLDALSAVLLLVVTG
                     VGFLIHVYSIGYMAHEEDHARFFVYLNLFMAMMLTLTLGNNLLVLFVGWEGVGLCSYL
                     LIGFFYDRPFDERTGLSCADAGRKAFIVNRIGDLAFLIGMLYIAFHFGTLNFGELDRA
                     IGQAGASGALLTGIGILLFIGACGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAG
                     VYMVCRMSTLYSAAPEAAAVVASVGAATALFAAILGVCATDIKKVLAYSTVSQLGYMF
                     AAAGVGAYIAGMFHLMTHAFFKGLLFLAAGSVIHGMSGVQDIRQMGGLKAKMPKTAWT
                     FVVGMLAISGVPIFAGFFSKDEILAGAWAGGHTVVWGVLLVAAAITAFYMTRV"
BASE COUNT          488 a         1264 c         1350 g          768 t
ORIGIN      
        1 ctgcggtctt ctcgccgcgg atgacctccg agtcgctgtt cgcctggagc gcgctgctgc
       61 aggccgccgg tggcgcgatc gtcgccgtcg cccgcgtcga acgcggcgcg gacgacgagc
      121 tgctgattcg ccgcgacagg ggggccaact cgaccggggc gcgttgggtg ctcggcgagg
      181 tcgggacgcc cgacgccgtg ctcgaggcgg tcggtgccgg acgggtcgat acgctgatcg
      241 tcttcggcga cccgctcgac ccggacgaga caccgaccgt gcccgaccaa gcgcgcgccg
      301 cgctgaccga gctggtctac gtcggcgcgt tcgcggacgc cgtcgcctcg cgggccggcg
      361 tcgtcctgcc cgtcgccgcg tgggccgagg aggacgggac gatcgtcaat ttcgaggggc
      421 ggatccagcg cgtgcggcgg gcgcacctgc cgcggggcga gcgccggccg gcctggcggg
      481 tggcggtcga cctcgcgcgc gcggccgaaa tcgaccttcc gcagtggagc tcggcggccg
      541 acgtgctggc ctcgctcggc accgcggtcg cggcctacga cggactggac gaagagacga
      601 tcgggctgtt gggcatcgcg gccaccgccg cggccgccgg cgcctgaccg aagggagcca
      661 agtgttcgac tggaccacgc tgctcatcgc cgcgatcgag attctcgtcg tggtcaacgt
      721 gctgatggtc ggcgtcgccg gcatgacctg ggtcgagcgc cggctgtccg cggtgatcca
      781 gttccgctgg ggacccaacc gcgtgggtcc ggccggcctg ctgcagccga tcgccgacgg
      841 catcaagttc ctgtggaagg aagagatgct gccgcgcgac gcctacatgc cggtgttcat
      901 cctggccccg gcgctggcaa tggtcacgtc gatcttcgcc ttctcggtca tcccgttcgg
      961 cccgacgatc cgcctgttcg gccggtcgat cgacctcgtg ctgctcgaca tgaacggcgg
     1021 catcctgttc gccttcgccg cgacctcgat cggcgtctat gccatcgtct gcgccggttg
     1081 ggcctcgcgc agcaagtacg cgctgatcgg cggtctgcgc tcgtcggcgc agatgatctc
     1141 ctacgagctg acgctcggac tgtcggtgat cggcgtgatc atcgccagcg gtacgctgcg
     1201 gccgacggtg atcgtcgagc agcaggccgg ctggttctgg agctggaaca tgctcggcgg
     1261 cggccagctg ctcggcttcc tgctgttcgt gatcgccggc ttcgccgaga ccaaccggct
     1321 tccgttcgac ctccccgagg cggagagcga gctggtggcc ggctaccaca ccgagtactc
     1381 gtcgatgaag ttctcgatgt tcttcatggc cgagtacatc gcgatgatca cgatgtcggc
     1441 gctggcgacg acgctgttcc tcggcgggta caaccccggt ttcccccccg aggcgatcgg
     1501 tctgtccgac ggttggccac tgtggatcct gcagctgttc agcttcgtcg tcaaggtcgg
     1561 gctgttcatg ttcttcttcg tttgggtgcg ttggacgctg ccgcggttcc gctacgacca
     1621 gctgatggac ctcggctgga agcggctgtt cccgctggcg ctgttcaaca tcttcctgac
     1681 cggtttcctc gtcgccttcg acgtgatctg aggagcgggg cgtgtcgctc gagctcgtgt
     1741 cgttcgtcgt gctgacggcg ctggcgctga tcaccgggct gacggtgatc cgccaccgca
     1801 atcaggtcgt cgcggcgctg gcgctggccg gcaacctggt ctcgatcgcc ggcttctata
     1861 tgttgctcaa cgcgcagttc ctcgcgttgc tgcaggtgat cgtctacgcc ggcgcgatca
     1921 tggtgttgat cctgttcgtg ctgatgctgc tcaacctgcc ggacgaggca cggcgcaagc
     1981 gcgacggttg gctgcagagc acggtcgggc tgctgttggc cgtggcgttc gtcgcgatcg
     2041 tcgggcgcgc gatcgccgag tcgaccgccg ccgacggttt tccggtcaat cccgccggct
     2101 tcggcacggt cgagtcggtc ggcacctcgt tgttctccga ttacttctac gcgttcgagg
     2161 cgatctcgct gctgctggtg gtggcgatgg tcggagccgt gctgctggcc aagcggaggc
     2221 tgtagcgatg accccgcaaa tggtgctggt cgtctccgcc gcgttgttcg cgatcggcgt
     2281 cgttggcgtg ctgagccgcc gcggcgcgat caccatcctg atgagcatcg agatcatgct
     2341 caacgccggc aacctcgcct tcatcgcctt ctcgcggatg caggatcagc ccgacggtca
     2401 ggtctacgcg ttcttcgtca tgacgctggc cgcggccgag gccgccgtcg gattggccat
     2461 cgtcatcgcc ctgtttcgct tgcgcgagtc caccgaggtg gacgagctga acctgatgaa
     2521 gtggtaggcc cgtgatgctc gacctgatct ggctcatccc gttgttgccg gcagccggtg
     2581 tgctgatcaa cggcttctgg ggacgtcgct tgtcgcgccc ggcggtcggc gcggtcggca
     2641 gcgcgaccgt cggcctggcg ctgctgttgg cggtcggcgc ggtctggcag ttgtcggggc
     2701 tcgaccccgc cctgcgggtc cacgaggtat cgctcggcac gtggatgccg ctcggtacgg
     2761 tcggcggcgc cgagctggtg atcgactggg gtttccggct ggacgcgctg tcggccgtgc
     2821 tgctgttggt cgtcaccggc gtcggctttc tgattcacgt ctacagcatc ggctacatgg
     2881 cgcacgagga agaccacgcg cggttcttcg tctatctgaa cctgttcatg gcgatgatgc
     2941 tgacgctgac cctgggcaac aacctgctgg tgctgttcgt cggttgggaa ggtgtcgggc
     3001 tgtgctccta tctgttgatc ggtttcttct acgacaggcc gttcgacgag cgcaccggtc
     3061 tgagctgcgc cgacgcgggc cgcaaagcgt tcatcgtgaa ccgcatcggc gacctggcct
     3121 ttctgatcgg catgctgtac atcgcgttcc acttcggcac actgaatttc ggcgagctcg
     3181 atcgagcgat cggccaggcc ggcgcgagcg gggcgctgct gaccggtatc ggcatcctgc
     3241 tgttcatcgg cgcctgcggc aaatcggcgc agatcccgtt gtacgtctgg ttgcccgacg
     3301 cgatggccgg cccgacgccg gtgtcggcgt tgatccacgc cgcgacgatg gtgaccgccg
     3361 gcgtctacat ggtctgccgg atgtcgacgc tgtacagcgc ggcacccgag gcggcggcag
     3421 tggtggcgtc ggtcggcgcg gcgacggcgc tgttcgcggc gatcctcggg gtctgcgcca
     3481 ccgacatcaa gaaggtgttg gcctactcga cggtcagcca gctcggctac atgttcgccg
     3541 ccgccggcgt cggcgcctac atcgccggga tgttccacct gatgacccac gcgttcttca
     3601 agggcctgtt gttcctcgcc gccggcagcg tgatccacgg catgagcggc gtgcaggaca
     3661 tccgccagat gggcgggctg aaggcgaaaa tgccgaagac cgcctggacc ttcgtcgtcg
     3721 gcatgctcgc catttcgggc gtgccgatct tcgccggctt cttctcgaag gacgagatcc
     3781 tcgccggcgc gtgggccggc gggcacacgg tggtttgggg agtgctgctg gtcgccgcgg
     3841 cgatcaccgc gttctacatg acccgcgtga
//
LOCUS       DASPGN010000108         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1423999, whole genome shotgun sequence.
ACCESSION   DASPGN010000108 DASPGN010000000
VERSION     DASPGN010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1423999"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VD788_03040"
     CDS             <1..837
                     /locus_tag="VD788_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HXV75267.1"
                     /translation="GSNEGYVLPLAEIRRKIEETYALGGNQILLQGGHNPQLKIDYYE
                     ELFRSLKQSFPDLWLHALSPPEIVHIYRASRITLDEALDRLVVAGLDSIPGGGAEILV
                     DSVRLRLATNKCSAGDWLEVMERAHRRGLRSTATMMFGHVETLDDRVEHLERIRSLQD
                     RTGGFTAFICWTYQAQNTDLGGRETTSADYLRTLAVSRLFLDNVDNLQASWVTQGPKL
                     GAASMAFGVNDMGSTMIEENVVSSAGTVYQMNEPEIVAAIRGAGFIPRRRDMTYTRLV
                     AP"
     gene            complement(839..3616)
                     /locus_tag="VD788_03045"
     CDS             complement(839..3616)
                     /locus_tag="VD788_03045"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXV75268.1"
                     /translation="MLKRHVAKVLPYVAIFAISGVVYLVGALEPLDRALIDAKFRIAA
                     RQVEPEVVIVAVDPPSLLESRVWPWSATDHATTAGNLLRAGARRIAFNVDVKDDTAAG
                     ETSAAGRLLQAMGARLVVPVSAQRMRGRFADDGSRPIPIAQPAWAAEAEPALHARLAD
                     SDGVVRRYAMRGTFLGRQLPSVAHALSGRGVPADDFYIDYGIEIDSIPVLSYAEVRRN
                     EFDPAVVRGRSVVISPTAVELDRPLAVPRYDQLAPGLVDALAVQSLASGRALERISPV
                     LSINLTLALCLMLAAVEFRFSWLRGLVWAVTVVAAIVVVSVGLQLFTPLLIDVSPMIL
                     VVAGAYGYRLARGYEFERLRPVVRDKQSRETETLMRHVVSHSFDAIVTLRDDGTTMTF
                     NRVAQKMFGYDEDEAIGRNISDFIRLPHAEDRMARQSEIRGTRRALYETEGFSKDGRR
                     FPVELAVTWIAAEGIRRRVMFMRDITERKAQQEALRYQATHDALTDLPNRSLLTERLE
                     HAVVLARQHAWPVAFLLLDLDRFKEINDTLGHHVGDELLRKIARRLESLVRESDTIAR
                     LGGDEFAVLMPATGLDNAQQMARGLALALEEPFQVEGLALQVESSVGITLYPEHGNTP
                     SALIRRADVAMYAAKKERQGVVVYDPEQDFTSIRLLALTGDLRRAIHDSELNMHFQPK
                     VSARTGRPLGVEALARWRHPTHGDIPPDEFVGLAEHSGLIQPLTQWVLETALGQCARW
                     ERKGISLSMSVNLSARNLLDESLPDWLKALLESTRLPVRRLTLEITESVIMEDPKRAM
                     DVLTELHKLGVRISIDDFGTGYSSLGYLKKLPATEIKIDKSFVMEMDRNPDDRTIVHS
                     TIELAHNLGLSVVAEGVSSRQTWEELKRLGCDTGQGYYFSRPLPADRLVRWYREALEG
                     IEAPAAAGDSG"
     gene            complement(3751..>4052)
                     /locus_tag="VD788_03050"
     CDS             complement(3751..>4052)
                     /locus_tag="VD788_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75269.1"
                     /translation="EEIALLGRNYRGDEARLAGLAHEVHAADGFEARGLERLDEFASR
                     DPAATARTKAYLRSGVLERIREQDGRRIPEFLDGWFREETRARVRAIVDDLRSRR"
BASE COUNT          674 a         1392 c         1348 g          638 t
ORIGIN      
        1 ggatcgaacg agggctacgt cctaccgctc gcggagatca ggcgcaagat cgaggagacc
       61 tacgcgctgg ggggcaacca gatcctgctg cagggcgggc acaacccgca gctgaagatc
      121 gactattacg aggaattgtt ccgctcgctc aagcagagct tccccgacct gtggctccac
      181 gcgttgtcgc cgcccgagat cgtccacatt taccgtgcct cgcgcatcac gctggacgag
      241 gcgctcgacc ggttggtcgt cgccggtctc gattcgatcc cgggtggcgg agccgagatc
      301 ctggtcgatt ccgtccgcct ccgactggcc accaacaagt gcagcgccgg cgattggctc
      361 gaggtgatgg agcgggccca ccggcgcggt ctgcgttcga ctgcgacgat gatgttcggt
      421 cacgtcgaga cgctcgacga tcgcgtcgag cacctcgaac gcatccgcag cttgcaggat
      481 cgcacgggtg ggttcaccgc cttcatctgc tggacctacc aggcgcagaa caccgacctc
      541 ggtggtcgtg aaacgacgag tgccgactac ctgcgcacgc tggccgtctc ccgtctgttt
      601 ctggacaacg tggacaacct ccaggcatcg tgggtcaccc agggacccaa gctgggggcg
      661 gcgtcgatgg ccttcggcgt caacgacatg ggcagcacga tgatcgagga gaacgtcgtc
      721 tcctcggccg gcacggtgta tcagatgaac gagccggaga tcgtcgcggc gatccgtggt
      781 gccggcttca tcccgcgtcg ccgcgacatg acctacaccc ggctcgtcgc gccgtagctt
      841 aaccgctgtc gcccgcggcg gccggggcct cgatcccctc cagcgcctcg cggtaccagc
      901 gaacgagtcg atcggcgggc agcggacgcg agaagtagta tccctgtccc gtgtcacagc
      961 ccaaccgctt cagctcttcc caggtctggc ggctggagac accctcggcg acgacgctca
     1021 aacccaggtt gtgggcgagc tcgatcgtcg agtggacgat cgtgcggtcg tcagggttgc
     1081 ggtccatctc catcacgaac gacttgtcga tcttgatctc cgtcgccggc agcttcttca
     1141 ggtagccgag cgaggagtaa ccggtgccga agtcgtcgat cgagatgcga acgccgagct
     1201 tgtgcagctc ggtcagcacg tccatcgcgc gtttcggatc ctccatgatg acgctctcgg
     1261 tgatctccag cgtcaagcgg cggaccggca ggcgggtcga ttcgagcaac gccttcaacc
     1321 agtccggcag actctcgtcc agcagattgc gcgccgagag attgaccgac atcgacaggc
     1381 tgatcccctt ccgctcccag cgggcgcact ggccgagcgc ggtctcgagc acccactgcg
     1441 tcaacggttg gatcaacccg ctgtgctcgg ccagcccgac gaactcgtcg ggcgggatgt
     1501 cgccgtgagt cgggtggcgc cagcgcgcca gcgcctcgac gccgagcggt cgcccggtgc
     1561 gcgccgacac cttcggctgg aagtgcatgt tcagctcgct gtcgtggatc gcgcgacgca
     1621 ggtcgccggt cagtgccagc aggcggatac tggtgaagtc ctgctccgga tcgtagacga
     1681 cgacaccttg acgctccttt ttcgccgcgt acatcgcgac gtcggctcgc cgaatcaacg
     1741 ccgacggcgt gttgccgtgc tcgggataaa gcgtgatgcc gacgctgctc tcgacctgca
     1801 gcgccaatcc ctcgacctga aacggctcct ccagcgccag cgccagcccg cgcgccatct
     1861 gctgcgcgtt gtcgagaccg gtggcgggca tcagtaccgc gaactcgtcc ccgccgagcc
     1921 gggcgatggt gtccgactcg cgcaccaacg actcgagccg ccgggcgatc ttgcgcaaca
     1981 gctcgtcgcc gacgtggtgg ccgagcgtgt cgttgatctc cttgaagcga tccagatcga
     2041 gcagcaggaa ggcgaccggc cacgcgtgct gccgggccag tacgacggcg tgctccagtc
     2101 gctcggtcaa cagcgagcgg ttgggcagat cggtcagcgc gtcgtgggtc gcctggtagc
     2161 gcagcgcctc ctgctgcgcc ttgcgctcgg tgatgtcgcg catgaacatc acgcggcggc
     2221 ggattccttc ggcggcgatc cacgtgacgg ccagctcgac cgggaaccgg cgaccgtcct
     2281 tggagaaccc ctcggtctcg tacagcgcgc ggcgcgtccc gcggatctcg ctctgtcgcg
     2341 ccatgcggtc ctccgcgtgc ggcagccgga tgaagtccga gatgttgcga ccgatcgcct
     2401 cgtcctcgtc gtagccgaac atcttctgag cgacgcgatt gaaggtcatc gtcgtgccgt
     2461 cgtcgcgcaa cgtgacgatc gcgtcgaagc tgtggctgac gacgtgacgc atcagcgttt
     2521 cggtctcgcg gctctgcttg tcgcgcacga ccggccgcaa ccgctcgaac tcgtagccgc
     2581 gcgccagccg atagccgtag gccccggcga ccaccagtat catcggcgag acgtcgatca
     2641 acagcggcgt gaacagctgc agtccgaccg aaacgacgac gatcgccgcc acgacggtga
     2701 cggcccaaac gaggccgcgc agccacgaga agcggaactc gacggcggcc aacatcagac
     2761 acagggcgag cgtcagattg atgctcaaca ccgggctgat ccgctcgagc gcgcggccgg
     2821 aggcgagcga ctggacggcg agcgcgtcga ccagacccgg ggcgagctgg tcgtaacgcg
     2881 ggacggcgag cgggcggtcc agctcgaccg cggtcgggct gatgacgacc gatcgcccgc
     2941 ggacgactgc cgggtcgaac tcgttgcggc gcacctcggc gtacgacagg acgggaatcg
     3001 aatcgatctc gataccgtag tcgatgtaga agtcgtcggc cggaacgcca cgccccgaca
     3061 gcgcgtgagc gacggacggc agctgccggc cgagaaaggt cccgcgcatc gcgtagcgtc
     3121 ggacgacgcc gtcgctgtcc gcgagtctcg cgtgcagcgc cggttcggcc tcggcggccc
     3181 acgccggttg cgcgatcgga atgggtcggg aaccgtcgtc ggcgaaacga ccccgcatcc
     3241 gttgggcgga gaccggaacg accagacgag cgcccatcgc ttgcagcagt cgaccggccg
     3301 cggacgtctc gcccgctgcg gtgtcgtcct tgacgtcgac gttgaacgcg atccgccggg
     3361 cgccggcccg cagcaggttg cccgccgtcg tcgcgtggtc cgtcgcggac cacggccaga
     3421 ctctcgactc gagcaggctc ggcggatcga cggcgacgat cacgacctcc ggctcgacct
     3481 gacgcgcggc gatgcgaaac ttggcgtcga tcaacgcgcg atcgagcggc tcgagcgcgc
     3541 cgaccaggta gaccacgccc gatatcgcga agatcgcgac atacggcagg accttcgcca
     3601 cgtgccgctt cagcatccgg tcaccggttc gcggaccagc atcgggaagg cccggttcgc
     3661 ccatccggag cttccaattc atatagtcag cgactagaag ctaggttcct cgccgccggc
     3721 gatcaagcgg gggcaaggcg gcgcgccggg tcagcggcgg gagcggaggt cgtcgacgat
     3781 cgcgcggacg cgggcccgcg tctcctcgcg gaaccagccg tcgaggaact cgggaatccg
     3841 gcgcccgtcc tgctcgcgga tacgctcgag cacgcccgag cgcaggtagg ccttcgtccg
     3901 cgcggtcgcc gccgggtccc gcgacgcgaa ctcgtcgagc cgttccagcc cgcgcgcttc
     3961 gaagccgtcg gcggcgtgga cctcgtgggc cagacccgcc aaccgggctt cgtcgccacg
     4021 atagttccgc ccgagcaacg cgatctcctc ca
//
LOCUS       DASPGN010000109         5058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_722131, whole genome shotgun sequence.
ACCESSION   DASPGN010000109 DASPGN010000000
VERSION     DASPGN010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722131"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1661
                     /locus_tag="VD788_03055"
     CDS             <1..1661
                     /locus_tag="VD788_03055"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HXV75270.1"
                     /translation="HPNRVVPTGRLIDEVWGEAAPDTARAALQGYVAGLRKALGSGAA
                     PLRTQAPGYVLEVGPEALDLDRFESMRAEARATADPARRAALLHAALALWRDVPLSEF
                     DGEPFAAVAAEQLEERRLGALEERIDADLAVGRHGEVVAELDALVAEQPYRERFRAQQ
                     MLALYRSGRQADALAAYRATREAFASGLGLEPGSELKALERAVLEQDPVLDPPRVEPG
                     PVSATRARHMPRDSRILVRAAAIGVVAVFGLALLVVLAADDPPSVEVLPNSVAVIDPA
                     ANKVIDSIQVGIRPGPIAAEAGSLWIGNLVDKTLMRIDTRSRRPAGTVSLDGRTPTGL
                     AVDRGTVWVAHGLLGSVSVVDAQFGDVTRAVPVTTPGAYWKTGAVAAGAGTIWAVFGD
                     GTLARLDRPGGDVRGRTQTNASPAGLAVGYGSVWVASASRSTVQRFSPISLAELSSST
                     VGSRPAAVALGFGDVWVASAGADLVYRIEVGGGSIAATIEVGEGPSAIAVDADTVWVV
                     NAEAGTVERIDPGTNTVTKTIEVGGAPAGVVVADGLVWVTVQPR"
     gene            complement(1666..3288)
                     /locus_tag="VD788_03060"
     CDS             complement(1666..3288)
                     /locus_tag="VD788_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV75271.1"
                     /translation="MLAAVVLAVVLALAGQAGALDSRSEVRDGGVFRIAYAIGSGIDS
                     LDPALAYTAPAWALLDTTCLRLMSFPDRPAPEAFRLVPEAAVAPPKVTGRSMTYTFTV
                     RKGLRFSDGKPVRATAFARAINRVLAPRMRSPWRTQLSDIAGAADVLAGRAATASGVS
                     AHGNTLTLRFTRPPAAVEVRMAMPYMCAVPPSLPIDPEGRAVIPAAGPFHVVEYRADE
                     RIVIRRNRFYGGNRPRHVDGFQVDLRAASPREMVLRIDRNEADWGHQVAGSFYDPSIQ
                     PPLWWKHGFNRERLYTQPGFTVRLLVLNASRPLFRDNPDLRRAVNLALDRSALSGGPV
                     ATRTDQYLPYAMPGFRDDDVYPLLGADLPRAKALARGNTRSGRAIMYTTDFAPPIQTA
                     MTVRQQLAEIGLDVEVKAIPEHIASAAYAGQLSRPGEPWDIALVLWGPPIPDPYAFLN
                     ALLETDFIGGTNLGGFSSSAIDAGLRQAARLTQSRARQRAYGEIDVRLARTEAPFAAI
                     DVLNEVTFVSNRVDPRCVVLRPALDLASVCLK"
     gene            3633..4400
                     /gene="ftsE"
                     /locus_tag="VD788_03065"
     CDS             3633..4400
                     /gene="ftsE"
                     /locus_tag="VD788_03065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HXV75272.1"
                     /translation="MSDSAAALLEGPVADENVAKPDVASAAAMIVLDDVRKVYEPDVV
                     AVDGVSFVIEKGEFVFVVGPSGSGKSTIIRLLLKELEPTTGRIIVGGRDLGRLKRSKV
                     PLLRRNVGCVFQDFKLLRDRSAFENIAYALRVQGEPNAAIRKKVPEVLALVGLAHKSA
                     SRPDELSGGEQQRVSIARAVVNHPPLLICDEPTGNLDPDTSVGIMQLLHRINTAGTTI
                     LMVTHDREMVDKMRKRVIALDTGKVVRDERRGGYETE"
     gene            4409..>5058
                     /locus_tag="VD788_03070"
     CDS             4409..>5058
                     /locus_tag="VD788_03070"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HXV75273.1"
                     /translation="MKLVVSEAFRSIGSNISTSFAATMTVLIGMFLLGLFIALGTWVV
                     SWSDHVKSQLEVKVFYSDAVTPKQVNAVQQYLGADDRVKEYQFVSKREALVRMQKRYP
                     ELTKNLPSNPLPASTEITPARAENVKAIAADLRAHRFAGVERVKDGQQTSKRILQVAR
                     VIEAVFLVAVLVLLVASTLLIANTIRLSIFSRRREIEVMKLVGATNWFVRGPFMLEG"
BASE COUNT          768 a         1692 c         1825 g          773 t
ORIGIN      
        1 tgcatccgaa ccgggtcgtc ccgaccggcc ggctgatcga cgaggtgtgg ggggaggcag
       61 cgcccgacac agcccgcgcc gcgctccagg gctacgtcgc cggcctccgc aaggcgctcg
      121 gcagcggcgc cgcgccgctc cgtacccagg ctcccgggta cgtgctcgag gtcgggcccg
      181 aagcgctcga cctcgatcgc ttcgagagca tgcgcgcaga ggcgcgcgcg acggccgatc
      241 ccgcgcgacg cgcggcgctc cttcatgccg cgctcgcact ctggcgcgac gtcccgctct
      301 cggagttcga cggcgagcca ttcgcggcgg tcgcggcaga gcagctcgag gagcgccggc
      361 tgggcgcgct cgaggagcgg atcgacgccg acctcgcggt cggacgtcac ggcgaggtcg
      421 tcgccgagct cgacgcgctc gtcgcagagc agccgtaccg cgagcggttc cgggcgcagc
      481 agatgctggc tctctatcgt tcagggcgtc aggcggacgc gctggccgca taccgggcga
      541 cgcgagaggc gtttgcctca gggctgggcc tcgagccggg ctccgagctc aaggccctcg
      601 agcgggcggt gctcgagcag gaccctgtgc tcgatccgcc gcgcgtcgag ccggggccgg
      661 tctcggcgac tcgtgcgcgg cacatgccgc gcgattcgcg catcctcgtg cgggcggcgg
      721 caatcggcgt cgtcgcggtg ttcggcctgg ccttgctcgt cgtgctcgcg gcggacgacc
      781 caccgtccgt cgaggtgctc ccgaactcgg tggccgtcat cgacccggcg gcgaacaagg
      841 tgatcgactc gatccaggtc ggcatccggc ccgggccgat tgcggcggaa gcgggcagtc
      901 tctggatcgg caacctcgtc gacaagacgc tgatgcgaat cgacacgcgg tcgcggcgtc
      961 cggcggggac ggtttccctg gacgggcgaa caccgacggg tctggccgtc gatcgcggca
     1021 cggtctgggt cgcgcacggc ttgcttggaa gcgtgagcgt ggtcgatgcg cagttcggag
     1081 acgtcactcg cgcggttcct gtcacgacgc ccggcgcata ctggaagacc ggcgccgtcg
     1141 cggctggtgc gggcacgatc tgggccgtct tcggcgacgg cacgctcgcc cgcctcgacc
     1201 gacctggagg cgacgtccgc ggcagaacgc agacgaacgc gtcgccggcc ggattggcag
     1261 ttgggtatgg gtcggtctgg gtcgcgagcg cgtcccggtc aacggtccag cgcttcagcc
     1321 cgatctcgct ggcagagctc agctcttcga cggtcgggag ccgtccggcg gccgtcgcgc
     1381 tcggcttcgg tgacgtctgg gtcgcgagcg cgggggcgga cctcgtgtac cggatcgagg
     1441 tcggtggtgg ttcgatcgcg gcgacgatcg aagtcggcga ggggccgagc gcgatcgccg
     1501 tcgacgccga caccgtctgg gtcgtgaacg ccgaagcggg aaccgtcgag cgcatcgacc
     1561 cggggacgaa caccgtaacg aagacgattg aggtgggcgg tgcccctgcc ggggtggttg
     1621 tcgcggacgg tctcgtttgg gtgaccgtcc agccgcgctg agtcgctact tcaggcacac
     1681 ggaggcgagg tccagcgccg ggcgcaggac gacgcaccgt gggtcgaccc ggttcgagac
     1741 gaacgtcact tcgttgagga cgtcgatcgc cgcgaacggc gcttcggtcc gcgcgagtcg
     1801 gacatcgatc tcgccgtagg cgcgctggcg cgcgcgcgat tgcgtcagcc gcgccgcctg
     1861 ccggaggccc gcgtcgatcg cgctcgacga gaatccgccg aggttggtgc cgccgatgaa
     1921 gtccgtctcc agcagcgcgt tgaggaacgc gtagggatcg gggatcggcg gcccccacag
     1981 cacgagcgcg atgtcccacg gctcgcccgg tcgcgaaagc tggccggcgt acgcggccga
     2041 ggcgatgtgc tccggaatcg ccttcacttc gacgtcgagg ccgatctcgg cgagctgctg
     2101 gcgcacggtc atcgcggtct ggatcggagg tgcgaagtcg gtcgtgtaca tgatggcccg
     2161 accgctgcgt gtgtttccac gggcgagtgc ctttgcccgc ggcaggtcag cccccaacag
     2221 ggggtacacg tcgtcgtcgc ggaagccggg catcgcgtag gggaggtact ggtcggtccg
     2281 ggtcgccacg gggccgccgc tgagcgcaga ccggtcgagt gcgaggttca cggcgcggcg
     2341 gaggtccgga ttgtctcgga agagggggcg tgacgcattg agcacgagca gtcgcacggt
     2401 gaatccgggc tgggtgtaga gccgctcgcg gttgaagccg tgcttccacc agagaggggg
     2461 ctggatcgat gggtcgtaga acgaacctgc tacctgatgt ccccaatccg cttcgttccg
     2521 gtcgatccgc aggaccattt cgcgcggcga tgcggcgcgt aggtcgacct ggaagccgtc
     2581 gacgtggcgt ggcctgttcc cgccgtagaa gcgattccgt cggatcacga tccgctcgtc
     2641 agctcggtac tccacgacgt ggaacggccc agcggccgga atcaccgctc ggccctcggg
     2701 atcgatcggc agggacggcg ggacagcgca catgtacggc attgccatcc tcacctcgac
     2761 tgcggccggc ggtcgggtga atcggagcgt aagggtgttg ccgtgtgcgc tgacaccgga
     2821 cgcggtcgcg gcgcggcccg cgagtacgtc ggcggcgccg gcgatatcgc tcagctgcgt
     2881 cctccagggc gatcgcatgc gcggagccag gacgcggttg atcgcccgcg caaacgcggt
     2941 cgctcgaacg ggtttgccgt cgctgaaccg caggcccttg cggacggtga acgtgtaggt
     3001 catcgacctc ccggtgacct tcggcggcgc gacagctgcc tcgggcacga gccggaacgc
     3061 ctcgggcgcc ggcctgtcgg gaaacgacat cagtcgcagg caggtcgtgt cgagcagcgc
     3121 ccaggcgggc gcggtatagg cgagggcggg atcgagcgag tcgatacccg agccgatcgc
     3181 gtacgcgatg cggaagacgc cgccgtcgcg gacctctgat cgcgagtcga gcgcacccgc
     3241 ctgaccggcg agcgcgagca ccacggcaag cacgacggcg gcgagcaacc acgaccgacc
     3301 atgtcgtgcg ccaggggtgc gggtggtgcc cacagtttgt tcactttgcc acgaagttcg
     3361 tccgatcaca acctcgcggc ggtgccgtcg cctggggtcg gtccatttca cgcctcggcg
     3421 catctcccat ctcctcctcg aacgtggtca cgcggatcca cggtgcgctt atgtcctgaa
     3481 ccggagccaa aggctcgcca aagagcagcc gctgccccgg tgagtccagc cgcaggactc
     3541 ggctacaatc ggccgaaccc aggaaccgga gcggccacgg ggaaggtcgc gtcgaacgcg
     3601 ctcgccggtg catggacgac ttgctcacac ccgtgtctga ctccgcagca gccctcctcg
     3661 aggggccggt cgccgacgag aacgtcgcga agcccgacgt cgcgtcggcg gcggcgatga
     3721 tcgtcctcga cgacgtccgc aaggtctacg agcccgacgt cgtcgccgtc gacggcgtct
     3781 cgttcgtgat cgagaagggg gagttcgtct tcgtcgtcgg gccgtcgggc tccgggaagt
     3841 cgacgatcat caggctgctg ctcaaggagc tcgagccgac gacgggccgg atcatcgtcg
     3901 gcggccgcga cctcggtcgc ctgaagcgct cgaaggtgcc gctcctgcgg cgaaacgtcg
     3961 gctgcgtctt ccaggacttc aagctgctcc gcgaccgctc ggcgttcgag aacatcgcgt
     4021 acgcgctccg ggtccagggc gagccgaacg ctgcgatccg caagaaggtg cccgaagtcc
     4081 ttgcgctcgt cggcctcgcg cacaagtccg cgtcccggcc ggacgagctc tcgggcggcg
     4141 agcagcagcg cgtctcgatc gcgcgcgccg tcgtcaacca tccgccgctc ctcatctgcg
     4201 acgagccgac cgggaacctc gaccccgaca cgtccgtcgg gatcatgcag ctcctccacc
     4261 ggatcaacac ggccggcacg acgatcctga tggtcacgca cgaccgcgag atggtcgaca
     4321 agatgcgcaa gcgcgtgatc gcgctcgaca ccggcaaggt cgtccgcgac gagcgacgcg
     4381 ggggctacga gaccgaatga cgagccggat gaagctcgtc gtctccgagg ccttccggtc
     4441 gatcgggtcg aacatctcga catcgttcgc ggcgacgatg accgtcctga tcgggatgtt
     4501 cctgctcggg ctgttcatcg cgctcggcac gtgggtcgtc tcgtggtccg accacgtcaa
     4561 gagccagctc gaggtgaagg tgttctactc cgacgccgtc acgccgaagc aggtcaacgc
     4621 ggtgcagcag tacctgggcg cggacgaccg ggtgaaggag taccagttcg tctccaagcg
     4681 cgaggcgctc gtccggatgc agaagcgcta ccccgagctg accaagaacc tgccgtcgaa
     4741 cccgctgccg gcctcgaccg agatcacgcc ggcgagagcc gagaacgtga aggcgatcgc
     4801 cgccgacctg cgggcgcaca ggttcgccgg cgtcgagcgg gtcaaggacg gccaacagac
     4861 ctcgaagcgg atcctccagg tggcgcgggt gatcgaggcc gtcttcctcg tcgccgtgct
     4921 cgtgctgctc gtcgcctcga cactgctgat cgccaacacg atccggctct cgatcttctc
     4981 gcgccggcgc gagatcgagg tgatgaagct ggtcggggcg acgaactggt tcgtgcgcgg
     5041 cccgttcatg ctcgaagg
//
LOCUS       DASPGN010000110         3671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_370334, whole genome shotgun sequence.
ACCESSION   DASPGN010000110 DASPGN010000000
VERSION     DASPGN010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370334"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VD788_03075"
     CDS             complement(<1..252)
                     /locus_tag="VD788_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75274.1"
                     /translation="MSGRRLLDSLDWPTIVATSALVTLGLFSIASATIGEDSGLWRAQ
                     LMWAAVAVVAAIVVVTVDYRLWAELAIAMHGIVLTLLVVV"
     gene            complement(227..2110)
                     /gene="mrdA"
                     /locus_tag="VD788_03080"
     CDS             complement(227..2110)
                     /gene="mrdA"
                     /locus_tag="VD788_03080"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXV75275.1"
                     /translation="MDYRERWELKDYLFGRRLERRILLFHVGLTVVLLGFLLDFWNLQ
                     AVHGEVYASLAENNRLRRIPLKPTRGEIYDRNDQVIASTRPSLDLVLRREGRVDLYPQ
                     LERLSPIIGVPIEQLVQRLDEIRGRPLFEPLLAKEDVSLAELARIEVRRDEFPSVEIR
                     QHERRHYPERELNAHAIGYVGEVSEAQLAAEEAGDGLAHGDIVGKSGIERAFDGPLRG
                     DRGLMVVSVNSIGRQMGNATVLEPADHGTAIHVTLDVRLQRALAEGLGQETGAGIFLD
                     AHTGEVLAMISTPSFDPNLFADGISRDNWDRIMTDPRRPLHDRAIASFYAPGSTFKVL
                     MAIAGLETGTIGPDDRVFCNGHTTIYGRRRLCWKRGGHGWVDVEQALAHSCNVFFYKL
                     GKDLGITEINRFGSAFGLGRLSGIDIPGEETGILGSEQWKLEVQREQWYPGDTISVAI
                     GQGLLAVTPVQMARMMAAVATGGRLPTPRLSKLTPQRTDTIRISDRTLGIVRRALRKA
                     VEEGTARRATEGEFTVAGKTGTAQVYKHSAGIDSDELPKAERDHAWFVGYAPADDPRI
                     AFAMVVEHGGHGGSTAAPIVRNVLQVFFSETPPGDERRASELRAGLSPGGEGADVGTQ
                     VAR"
     gene            complement(2145..2639)
                     /locus_tag="VD788_03085"
     CDS             complement(2145..2639)
                     /locus_tag="VD788_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75276.1"
                     /translation="MRLVTVIVVLAVCLAFRIALGAIWPGAARYVDVMIVPVVWYAIA
                     GSQRSAMLVGCAAGLLQDSWFQTGAFGTSGFKKTLVGWLLGGLGSRFDLNNQFNRFVF
                     GTLASVSDSLLGLVLHAVLDRPGGQAEPLEIGTRALVSGLLVSLAFGLTDRLRVRRSR
                     RSLA"
     gene            complement(2636..3493)
                     /gene="mreC"
                     /locus_tag="VD788_03090"
     CDS             complement(2636..3493)
                     /gene="mreC"
                     /locus_tag="VD788_03090"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXV75277.1"
                     /translation="MPVLSTTRTNVILLVGLLFGQLMLMAGSAGERPEAANLRGWFSR
                     VTSPIITAAGTIGGAVGDFVDNAGDLIAADSRNRELESELARLRTELRLVREASNENI
                     RLRRLLEMRENLVPVSIGASVVTANLTGQEMGVVIDRGAADGLRLDLPAVAWGGAVGR
                     IVSLDATHAVLRLLTDPNSGVAGIVQGSGAQGMVVGQGTDVLEMLYVPRFSSVIHGDR
                     VVTSGLDGIFPRGFGIGEVIEIQEKPDGSQTILLRPELDYHKMKELLVLAEPVGGELL
                     SHSGVDRPR"
BASE COUNT          609 a         1346 c         1159 g          557 t
ORIGIN      
        1 cacgacgacc agcaacgtca gcacgatacc gtgcatcgcg atggccagct cggcccacag
       61 tcggtagtcg acggtgacga cgacgatcgc cgccacgacg gcgacggccg cccacatcag
      121 ctgcgcgcgc cacagcccgg agtcttcgcc gatcgtcgcc gacgcgatcg agaacagacc
      181 gagggtcacg agcgccgagg tcgcgacgat cgtcggccaa tccagactat cgagcaacct
      241 gcgtcccgac atccgctccc tcgccgcccg gcgacagacc cgcgcgcagc tcgctcgcgc
      301 gccgttcatc gcccggtggt gtctccgaga agaagacctg cagcacgttg cgcacgatcg
      361 gcgcggcggt cgagccgccg tggcctccgt gctcgacgac catggcgaag gcgatccgcg
      421 ggtcgtcggc cggcgcatag ccgacgaacc aggcatggtc gcgctccgcc ttgggcaatt
      481 cgtccgagtc gatcccggcg gagtgcttgt agacttgggc cgttccggtc ttgccggcaa
      541 cggtaaactc gccctcggtc gcacggcggg ccgtcccctc ttccaccgcc ttgcgcagcg
      601 cgcgccgcac gatcccgagt gtacggtcgg agatccggat cgtgtcggtg cgctgcggcg
      661 tcagcttcga caaccgcggg gtcggcaacc ggccgccggt cgccaccgcc gccatcatcc
      721 gcgccatctg caccggggtc accgccagca ggccctgtcc gatcgcaacc gagatcgtgt
      781 cgccggggta ccactgctcg cgctgcacct cgagcttcca ttgctccgag ccgagaatcc
      841 ctgtctcctc accgggaatg tcgatcccac tcagccggcc gaggccgaac gccgacccga
      901 accggttgat ctcggtgatg ccgagatcct tgcccagctt gtagaagaag acgttgcacg
      961 agtgcgccaa cgcctgctcg acgtcgaccc atccgtgacc gccgcgcttc cagcacaacc
     1021 ggcgccggcc gtagatcgtc gtgtgcccgt tgcagaacac ccgatcgtcg ggaccgatcg
     1081 ttcccgtctc gagcccggcg atcgccatca gtaccttgaa cgtcgagccg ggcgcgtaga
     1141 agctggcgat cgcccgatca tgcagcgggc ggcgtgggtc ggtcatgatc cgatcccagt
     1201 tgtcgcggga gatcccgtcg gcgaacaggt tcgggtcgaa cgacggcgtg gagatcatcg
     1261 ccagcacttc gccggtgtga gcgtcgagga agataccggc gcccgtctcc tgccccagcc
     1321 cttcggccaa ggcgcgctgc agtctcacgt cgagcgtgac gtggatcgcg gtcccgtggt
     1381 ccgctggctc gagcaccgtt gcgttgccca tctgccgccc gatgctgttc accgagacga
     1441 ccatcagccc gcggtcaccg cgcagcgggc cgtcgaacgc acgctcgatc ccgctcttgc
     1501 cgacgatgtc gccgtgagcc agcccgtcgc cggcctcctc tgccgcgagc tgggcctcgc
     1561 tcacctcgcc gacgtagccg atcgcatggg cgttcaattc gcgctcggga tagtgccgtc
     1621 gttcgtgctg gcggatctcg accgacggga actcatcacg ccgaacctcg atccgcgcca
     1681 gctcggccag gctgacatcc tccttggcca gcagcggctc gaacaacgga cggccgcgaa
     1741 tctcgtccag acgctgcacg agctgctcga tcggaacgcc gatgatcggg gacagtcgct
     1801 cgagctgcgg gtacagatcg actcttccct cccgccgcag caccaggtcg agcgacgggc
     1861 gggtcgacgc gatcacctgg tcgttgcgat cgtagatctc gccgcgcgtc ggcttgagcg
     1921 gaatccgccg aagccggttg ttctccgcga ggctggcgta gacctcgccg tgaaccgctt
     1981 gcaggttcca gaagtcgagc aggaatccga gcaacaccac ggtgaggccg acgtgaaaca
     2041 acaggatccg tcgctcgagc ctgcgcccga acaagtaatc cttcagttcc cagcgttctc
     2101 tgtaatccac cgccttctct tgccgcctac cgtcagtcta gcgctcaagc cagcgaacgt
     2161 ctgctgcgcc gcacgcgcag ccggtcggtg aggccgaacg ccagcgacac cagcaacccg
     2221 ctgacgagtg cgcgcgtgcc gatctcgagc ggctcggcct ggccccccgg ccggtccaga
     2281 acggcatgca gcaccaaccc gagcaggctg tccgacaccg atgccaacgt gccgaagacg
     2341 aaccggttga actggttgtt gaggtcgaag cgcgagccga gtccgccgag gagccacccg
     2401 accagggtct tcttgaagcc gctcgtgccg aacgcgccgg tctggaacca ggagtcctgc
     2461 agcagacccg ccgcgcagcc gaccagcatc gcggagcgct gggacccggc gatcgcgtac
     2521 cacacgaccg ggacgatcat cacgtcgacg tagcgggccg ccccgggcca gatcgcgccg
     2581 agcgcgatcc ggaacgccaa gcagacggcg agcacgacga tgaccgtgac cagtctcatc
     2641 gcggccggtc gactccgctg tgcgacagca gctcgccgcc gaccggctcc gccagcacca
     2701 gcagctcctt catcttgtgg taatcgagct cgggccgcag caggatcgtt tggctgccgt
     2761 ccggcttctc ctgaatctcg atcacctcgc cgatgccgaa cccgcgcggg aagatcccgt
     2821 cgaggccgga ggtgacgacg cggtcgccgt ggatcacgct ggaaaagcgg ggcacgtaga
     2881 gcatttcgag cacgtcggtc ccctgcccga cgaccatccc ttgggcgccg ctgccctgga
     2941 cgatgccggc gacgccgctg ttcggatcgg tcagcagacg caagaccgcg tgcgtcgcgt
     3001 cgagactgac gatgcgcccg accgcaccgc cccaggcaac cgccggcaag tcgagccgca
     3061 gaccgtccgc ggcgccgcga tcgatgacca cgcccatttc ctgtcccgtc aggttcgccg
     3121 tgacgacgct cgcaccgatc gacaccggca ccaggttctc gcgcatctcg agcaagcgcc
     3181 gcagccgtat gttctcgttc gacgcttcgc ggaccaggcg aagctcggtg cgaagccgtg
     3241 ccaactccga ctcgagctcc cgattccgcg agtcggcggc gatcaggtcg cccgcgttgt
     3301 cgacgaagtc gccgaccgcg ccaccgatcg tccccgcggc ggtgatgatc ggcgaggtga
     3361 cccgcgagaa ccagccgcgc aggttggccg cctccggccg ttcgcccgcg ctacccgcca
     3421 tcagcatcaa ctggccgaag agcagaccga ccagcaggat cacgttggtc cgcgtcgtgc
     3481 tcaagaccgg catctctcgc cctccgcccc ggccgacgcc gcggggctcg ctgcgcgtcc
     3541 gccgggccgt cgccctagtc gagcgatacc ttccgcagca ggttgaagtc gctgaggacg
     3601 cggccggtac ccatcaccac cgaggtcaac gggtcgtcgg cgatcgagac cggcaggccg
     3661 gtttcctcgc g
//
LOCUS       DASPGN010000111         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_488039, whole genome shotgun sequence.
ACCESSION   DASPGN010000111 DASPGN010000000
VERSION     DASPGN010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488039"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..>2785
                     /locus_tag="VD788_03095"
     CDS             20..>2785
                     /locus_tag="VD788_03095"
                     /inference="COORDINATES: protein motif:HMM:NF039608.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75278.1"
                     /translation="MTQQPPGPHWDLTGASCSDGSDPAAIELLPGETVVCTFENTKRG
                     QLIVSKTAVGGDGTFAFDSATLGGFALPTSAGSASTTFPLLVPGVYDVTESALAEWDL
                     TSTACSDGSAPGAIVVDPGETVTCVFENTKRGVLVVVKETVGGDGIFSFASGTLGAFE
                     LATTGGAASETFAGLPPGVYDVAESDPAPAWDLTAASCSDGSDPSSIAIDPGETVTCT
                     FRNTKRSRLIVAKTTIGDDGTFAFLSASLGAFELGTDSGAGSVAFDDLRPGIYDVSES
                     DPTPAWDLTAVSCSDGSAPGAIELSPGESVTCTFENTRRGRIVVVKTSIGGDGAFEFA
                     SQTLGAFELVTADGAASIQFDDLPPGPYDVSELDAGSSWDLTAVACSDGSDPASIDLD
                     PGETVSCAFENTKRGRLTIVKNAIGGDGMFGFTSAALGAFELETSNGTAVTTFDDLIA
                     GSYDVTELDQDPDWDLTAASCSDGSLPAAVRIDPGEQVSCTFENTKRGRLVVEKVSIG
                     SDGTFAFDSATLGDFALITSSGAASHGFDHLLPGVYDVHEVDGDGTWDLTGASCDDGS
                     PLAAIDIAPGETVTCTFANTKRARLTIVKRTIPDDPQDFHFTASGDSFADTFSLDDDG
                     DASDALSDRFSAHLVPGDYLLVESDPGPLWDAAGVVCTDGSGADTGDVGLRTAALGLA
                     AGADVTCVFTDVKRGRIVVDKVVEQVPFVPDYDRAQWPFDFSTDYAGSFSLRHGESHD
                     SGYIRSNQRYLVSEAGQRGWVVGHECRYPDGSMTTGGATAAIDLAPGAEVSCTFFNVM
                     RLHPGSSGFWRNWRNQYASWQLEDALADGFSSSPIYDDLFDPATGVLLPSAVDTVDRI
                     YDNRQDQDQPTQPLLREITSTMFNLTVSNSTDPVVAAMQQNDDICYECLLDLSSIPGA
                     DELLERWS"
BASE COUNT          434 a          962 c          953 g          436 t
ORIGIN      
        1 gcccgccgga acgtactccg tgacgcagca accgcccggg ccgcactggg acctgaccgg
       61 tgcctcgtgc agtgacggca gcgaccccgc cgcgatcgag ctcttgcccg gcgagacggt
      121 cgtttgtacg ttcgagaaca ccaagcgcgg acagctgatc gtgtcgaaga ccgccgttgg
      181 cggcgacggc accttcgcgt tcgacagcgc gacgctgggt ggcttcgcgc tgccgacgtc
      241 ggccggtagc gcgagcacga cctttccgct gctcgtcccc ggcgtctacg acgtgacgga
      301 aagcgcgctg gccgaatggg acctcacctc gaccgcctgc tcggacggca gcgcgcccgg
      361 ggcgatcgtc gtcgatcccg gtgagacggt gacctgcgtc ttcgagaaca cgaagcgcgg
      421 cgtgctggtc gtcgtgaagg agaccgtcgg tggggatggg atcttctcgt tcgccagcgg
      481 tacgctcggc gcgttcgagc tggcgacgac gggcggtgcg gcgagcgaga ccttcgccgg
      541 gctgcctccc ggcgtctacg acgtggccga gtccgacccc gcgcccgcgt gggatctcac
      601 cgcggcctcc tgcagcgacg gcagcgatcc gtcctcgatc gcgatcgacc cgggagagac
      661 ggtgacgtgc acgttccgca acacgaagcg gtcgcggctg atcgtcgcca agacgacgat
      721 cggcgacgat gggacgttcg cgttcctgag cgcctctctc ggcgcgttcg agctcgggac
      781 cgactcgggc gcgggcagcg tcgcgttcga cgacctccgc cccgggattt acgacgtgag
      841 tgagagcgac ccgaccccgg cgtgggatct caccgcggtc tcctgcagcg acggcagcgc
      901 ccccggcgcg atcgagcttt cgcccggcga gagcgtgacc tgcaccttcg agaacacccg
      961 gcgcggccgg atcgtcgtcg tcaagaccag catcggcggc gacggtgcct tcgaattcgc
     1021 gagccagacg ctcggcgcgt tcgagctggt gacggcggac ggtgcggcga gcatccagtt
     1081 cgacgatctg ccgccggggc cgtatgacgt cagcgagctg gacgctggat cgagctggga
     1141 cctgacggcc gtcgcctgca gcgacgggag cgaccccgcg tcgatcgatc tcgatccggg
     1201 agagacggtc agctgcgcct tcgagaacac caagcgtggg cggctgacga tcgtcaagaa
     1261 cgcgatcggc ggagacggca tgttcggctt caccagtgcc gcgctcgggg cgttcgagct
     1321 cgagacgtcg aacggcacgg cggtgacgac cttcgacgac ctgatcgccg gcagctacga
     1381 cgtcaccgaa ctcgatcaag atccggactg ggacttgacc gcggcctcct gcagcgacgg
     1441 gagcctgccc gcggccgttc gcatcgatcc gggcgagcag gtgagctgca cgttcgagaa
     1501 caccaagcgc ggccggctgg tggtcgagaa ggtgtcgatc ggcagcgacg gcaccttcgc
     1561 gttcgacagc gcgacgctgg gtgacttcgc gctgatcacc tcatccggcg cggcgagcca
     1621 cggcttcgac cacctgttgc ccggcgtcta cgacgtgcac gaggtcgacg gggacgggac
     1681 ctgggacttg accggcgcct catgcgacga cggcagcccg ttggcggcga tcgacatcgc
     1741 ccccggtgag accgtcacct gcaccttcgc caacaccaag cgcgcgcggt tgacgatcgt
     1801 caagcgcacg attcccgatg acccgcagga cttccatttc accgcttccg gcgactcgtt
     1861 cgccgacacg ttctcgctcg acgacgacgg cgatgcgagc gacgcgttga gcgaccggtt
     1921 cagcgcccac cttgttcccg gcgactattt gctcgtcgag tccgaccccg gtccgctgtg
     1981 ggacgccgcg ggcgtcgtct gcaccgacgg gtcgggggcc gacacgggcg acgtcgggct
     2041 gcggaccgcc gcgctcgggc tcgccgcggg cgccgacgtc acctgcgtct tcaccgacgt
     2101 caagcgcgga cggatcgtgg tcgacaaggt tgtcgagcag gttccgttcg tcccggacta
     2161 cgaccgcgca cagtggccgt tcgatttctc gaccgactac gccggctcgt tctcgctgcg
     2221 gcacggcgag agccacgaca gcggctacat ccgttcgaat cagcgctacc tcgtgtcgga
     2281 ggccgggcaa cgcggctggg tcgtcgggca cgagtgccgc taccccgacg ggtcgatgac
     2341 caccggcgga gcgaccgccg cgatcgatct cgccccgggc gccgaggtct cctgcacgtt
     2401 cttcaacgtg atgcggttgc atcccggctc ctccggcttc tggcgcaact ggcgcaacca
     2461 gtacgcctcg tggcagttgg aggacgcgct ggccgacggc ttctcttcgt cgccgatcta
     2521 cgacgacctg ttcgacccgg cgacgggggt gctgctcccg tcggcggtcg atacggtcga
     2581 ccggatctac gacaaccgac aggaccagga ccagccgacg cagccgctgc tgcgggagat
     2641 caccagcacg atgttcaacc tgacggtcag caacagcacc gatccggtcg tcgccgcgat
     2701 gcagcagaac gacgacatct gttacgagtg cctgctcgac ctgtcttcga tcccgggagc
     2761 cgacgagctg ctcgagcgct ggtcc
//
LOCUS       DASPGN010000112         4964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1073633, whole genome shotgun sequence.
ACCESSION   DASPGN010000112 DASPGN010000000
VERSION     DASPGN010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4964
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073633"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1661)
                     /locus_tag="VD788_03100"
     CDS             complement(54..1661)
                     /locus_tag="VD788_03100"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HXV75279.1"
                     /translation="MADPPPAFVESFSADERRALAPYFTNVDRPVFAPINLPEVVKGA
                     LFARYSRSAKSLRRLFLDEFLDELGGAAVPVAVGVERAERLYDKVFVEYGDDSVAQLG
                     GVHLACENVSNVLTKILEWGRLMAYLEQSTRYVPYTGRPGGRFRYHVPAELVGTPAAA
                     GYSAALDLAFSTYGKWLEPMERYLETRHPRADDVSPAVYRRTIRAKALDALRGLLPAA
                     TLANVGIFGTGQAYEALLLRMRANPLREVQHCAELMLNELREVVPAFLRRVDKPERGG
                     RWSRYLADSRRETARVAAEVLAGVTADPPDEVTLSDFDPAGETKVVAAAMYAVSTLPD
                     SVLAARARTMSHEERLRVLTAYVGDRRNRRHKPGRAFERTSYRFDVLTDYGAFRDLQR
                     HRMLTLEWQALSPDHGYIVGDAVEQAGALDDWRRVMDACARVYHEIVRESGNREIAAY
                     AVPMAHRVRFYMEMNAREAMHTIELRTGPQGHPSYRRVCQRMHRLIAEVAGHRAIADS
                     MRYADHGEVELERLDAERAAEQRRGDG"
     gene            complement(1672..2232)
                     /locus_tag="VD788_03105"
     CDS             complement(1672..2232)
                     /locus_tag="VD788_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXV75280.1"
                     /translation="MNTFDIILLGFACALVVLGMLKGLVRILIGMAALVAAFALAARH
                     HAGLAQRLSALEIGVEALRLLAYLLIFVGVMLAGGLVAWLLRRVLKTAMLGWADRLGG
                     AALGLIAALLAAALVILPVVAYSPYGGQLLGNSLLAPYVSVVADVATRLAPADLSQRY
                     RDKIEELRRHWRDRYVESLPVDPSRV"
     gene            complement(2229..3677)
                     /gene="purF"
                     /locus_tag="VD788_03110"
     CDS             complement(2229..3677)
                     /gene="purF"
                     /locus_tag="VD788_03110"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXV75281.1"
                     /translation="MCGFIGIIGSDSAVHEIYDGLLAVQHRGQDAAGIITYDGRFHVK
                     KGEGLVRDIFSAENVRRLSGSVGVGHVRYPTVGSGGGEDAQPFTVNYPYGIVMAHNGN
                     VANYEELRGELAVEGLRHLYSACDVEVVLNVFANELAAAGRDGFSIGAYHAAVRAVYR
                     RVRGAYSVVGFIAGHGLFAFRDPYGIKPIAVGRRVRDGKAAYAVASESVVLDTIGYDV
                     LPTRGPGEAMFIDLDGRTHFEQLSQPTHRPCVFEFVYFARPDTYIEDISVYAARLRMG
                     RRLARGFRDAGLEADVVIPVPDSACTAALALAQELGLPYREGLVKNRYVGRTFIMPDD
                     GARRSSVRRKMNTIRDEFLGKRVLLVDDSIVRGNTSRQIVQLARAAGATKVYFASTAP
                     PLVHPCVYGIDMSTRREFVARDRTPQEVARQIDADAVIYQTLDDLTQSVLEDRPDIER
                     MCTACFSGDYPTGDITPEMLLQIEAERTLHGK"
     gene            3836..4963
                     /gene="mqnE"
                     /locus_tag="VD788_03115"
     CDS             3836..4963
                     /gene="mqnE"
                     /locus_tag="VD788_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HXV75282.1"
                     /translation="MTDLVETAACEAGLGDVYRKVAAGERLTFDDGVRLFESRELMTI
                     GAMANLVRERKNGDDAYFVRNMHLNPTNVCTVDCKFCGFYRPYRHKQQGWTWSLDRCL
                     AEVRARLAESLTEVHIVGGHNPDYPYEFYTDLIRGIRELDPRLHVKAFTAAEYDFFAR
                     RFKKPLERVFRDFIDAGLGSLPGGGAEVLVERVRREIYPKKISAERWLEIVRLAHEFG
                     LRSNATLLYGHVERVDERIEHLCRLRALQDRTGGFMCFIPLAFHPENTELSQLPGPTG
                     FDDLMTIAVSRLMLDNFDHVKAYWIMLTPRIAQTALNMGADCIDGTVVEEKIVHMAGA
                     KTPVGLEMDELLRLIREAGRLPVQRDSEYNELRRFTEHAVA"
BASE COUNT          826 a         1672 c         1697 g          769 t
ORIGIN      
        1 cgtggccgcc gcggtgcggc ggctgttcgc actggccagc ggcgcgaagt agctcagccg
       61 tcgccgcggc gttgctcggc cgcgcgctcg gcgtcgagcc gttccagctc gacctcgccg
      121 tggtcggcgt agcgcatgct gtcggcgatc gcgcgatgac cggcgacctc ggcgatcaaa
      181 cggtgcatcc gctggcagac gcgacggtag ctggggtggc cctgcggccc ggttctcagc
      241 tcgatcgtgt gcatcgcctc gcgcgcgttc atctccatgt agaaccggac ccggtgggcc
      301 atcggcaccg cgtaggcggc gatctcgcgg ttgccggact ctcggacgat ttcgtggtag
      361 acgcgagcgc acgcgtccat cacccgccgc cagtcgtcga gcgcgcccgc ctgctcgacc
      421 gcgtcgccga cgatgtagcc gtggtccggt gacagggcct gccactccag cgtcagcatg
      481 cgatgacgct gcaggtcgcg gaacgcaccg tagtcggtca gcacgtcgaa gcgatagctg
      541 gtccgctcga aagcccgacc cggcttgtgg cgccggttcc gtcgatcgcc gacgtaggcc
      601 gtcaggacgc gcagccgttc ctcgtgcgac atcgtgcggg cccgcgcggc caacaccgag
      661 tcgggcagcg tcgagacggc gtacatcgcc gccgcgacga ccttcgtctc gcctgccgga
      721 tcgaagtcgc tgagcgtgac ctcgtccggg ggatcggcgg tcactccggc gagtacctcg
      781 gcggcgacgc gcgcggtttc ccggcggctg tcggccagat accggctcca gcgtccgccc
      841 cgctccggtt tgtcgacccg gcgcaggaac gcgggcacca cctcccgcag ttcgttgagc
      901 atcagctcgg cgcagtgctg cacctcgcgc agcgggttgg cccgcatccg cagcagcagc
      961 gcctcgtaag cctgaccggt gccgaagatg ccgacgttgg ccagggtggc ggcgggcagc
     1021 agtccgcgca gcgcgtcgag cgccttggcg cggatcgtgc ggcggtagac cgccggggag
     1081 acgtcgtccg cgcgcgggtg acgcgtttcg agataccgct ccatcggctc gagccacttg
     1141 ccataggtcg aaaaggcgag gtcgagcgcg gcgctgtagc cggccgcggc cggggtgccc
     1201 accagctcgg ccgggacgtg atagcggaag cgaccgccgg ggcggccggt gtacggcacg
     1261 tatcgcgtgg actgctcgag gtaggccatc agccgccccc actcgaggat cttggtcagc
     1321 acgttcgaga cgttctcaca ggcgagatgc acgcccccga gttgggcgac cgaatcgtcg
     1381 ccgtactcga caaacacctt gtcgtagagc cgctccgccc gttcgacgcc gaccgcgacc
     1441 ggcacggccg ctccccccag ctcgtcgagg aactcgtcga gaaacagccg tcgcagcgac
     1501 ttggcgctgc gcgaatagcg cgcgaacagc gcgcctttga ccacctccgg caggttgatc
     1561 ggcgcgaaga ccgggcggtc gacgttggtg aagtacggcg cgagcgcgcg ccgctcgtcg
     1621 gcgctgaacg actcgacgaa agctggcggc gggtcggcca agtcgttccc ctcagacgcg
     1681 cgacgggtcg accggcagtg attcgacgta acgatcccgc cagtggcggc gcagctcttc
     1741 gatcttgtcg cggtagcgtt gagacagatc ggcgggggcc agccgggtgg ccacgtccgc
     1801 gacgaccgag acgtaggggg ccagcagcga gttcccgagc agctggcccc cgtacggtga
     1861 gtaggcgacg accggcagga tcaccagcgc cgccgccagc aacgcggcga tcaatccgag
     1921 cgccgccccg cccaagcggt cggcccagcc gagcatcgcc gtcttcaaca cgcgacgcag
     1981 cagccaagcg acgagaccgc cggccaacat cacgccgacg aagatcagca ggtaggccag
     2041 cagccgcagc gcctcgacgc cgatctcgag cgcgctcagc cgttgggcca gtcccgcgtg
     2101 gtggcgggcg gcgagcgcaa aggcggcgac cagcgccgcc ataccgatca ggatgcgaac
     2161 cagccccttg agcatgccca acacgacgag ggcgcaggcg aaacccaaca gaatgatgtc
     2221 gaaggtgttc atttgccgtg cagcgtcctc tcggcttcga tctgcagcaa catctccggc
     2281 gtgatgtcgc ccgtcggata gtcgccggag aaacaggcgg tgcacattcg ctcgatgtcc
     2341 gggcggtcct cgagaaccga ctgggtcagg tcgtcgagcg tctggtagat caccgcgtcg
     2401 gcgtcgatct gacgcgcgac ttcctgcggc gtccgatcgc gcgcgacgaa ctcgcgccgc
     2461 gtgctcatgt cgatcccgta gacgcaggga tggaccagcg gcggtgcggt cgaggcgaaa
     2521 tagaccttgg tcgcgccggc ggcacgcgcg agctggacga tctgacggct ggtgttgcca
     2581 cgcacgatcg aatcatcgac cagcagtacg cgcttgccga ggaactcgtc gcgaatcgta
     2641 ttcatcttgc ggcgaaccga gctccggcgc gctccgtcgt ccggcatgat gaacgtccga
     2701 ccgacgtagc gattcttgac cagaccctcg cggtacggca gtcccagttc ctgggccagc
     2761 gccagcgccg ccgtgcaggc cgaatccggt accgggatga cgacgtcggc ttcgagcccg
     2821 gcgtcgcgaa agccgcgggc caaccgccgc cccatgcgca gccgtgccgc gtagaccgaa
     2881 atatcctcga tgtaggtgtc gggacgggcg aagtagacga actcgaagac gcacgggcga
     2941 tgcgtcggtt gactcagctg ctcgaagtgg gtgcggccgt cgaggtcgat gaacatcgcc
     3001 tcgcccggac cgcgcgtcgg cagcacgtcg tagccgatcg tgtcgagcac gacgctctcc
     3061 gacgccaccg cgtaggccgc cttgccgtcg cgaacgcgac gtccgaccgc gatcggcttg
     3121 atgccgtacg ggtcgcgaaa ggcgaacaat ccgtggccgg cgatgaagcc gacgacgctg
     3181 tacgcgccgc ggacgcgccg gtacaccgcg cgcacggccg cgtggtacgc gccgatcgag
     3241 aacccgtcgc gccccgcggc ggccaactcg ttggcgaaga cgttgagcac gacctcgacg
     3301 tcgcacgccg agtagaggtg gcgcagtcct tcgacggcga gctccccgcg aagctcctcg
     3361 taattggcga cgttcccgtt gtgtgccatc acgatgccgt acgggtagtt gacggtgaac
     3421 ggctgggcgt cttcgccgcc cccggagccg acggtcgggt agcgcacgtg acccacgccg
     3481 accgaaccgc tgaggcggcg gacgttctcg gccgagaaga tgtcacggac caggccctca
     3541 cccttcttca cgtggaaccg gccatcgtag gtgatgatcc cggcggcgtc ctgcccgcgg
     3601 tgctggacgg ccagcagacc gtcgtagatc tcgtggacgg cggagtccga gccgatgatg
     3661 ccgatgaaac cgcacatggc cgcgtcggtc ccccgaaaag cggacatggt aacaccgatc
     3721 ggcagacccg cgcccgtcga ttgaccggcg aggggtgcct ttctataatg cgttttcagt
     3781 gacttacgct gggtggtgat gtgatgccgg gtgcggccct ccacgggtac ggtcgatgac
     3841 ggacctggtc gaaacggcgg cgtgcgaggc cggcctgggt gacgtctacc gcaaggtggc
     3901 ggccggcgag cgcctgacgt tcgacgatgg cgtgcggctg ttcgagtctc gcgagctgat
     3961 gacgatcggc gcgatggcca atctggtgcg cgagcgcaag aacggcgacg atgcctactt
     4021 cgtccgcaac atgcacctca acccgaccaa cgtctgcacg gtcgactgca agttttgcgg
     4081 cttttaccgg ccgtaccgcc acaagcagca gggctggacc tggagcctcg atcgctgcct
     4141 ggccgaggtt cgcgcgaggc tcgccgagtc gctgactgag gtccacatcg tcggcgggca
     4201 caacccggac tacccctacg agttctacac cgacctgatt cggggcattc gcgagctcga
     4261 cccgcggctg cacgtcaagg cgttcaccgc cgccgagtac gatttcttcg cccggcgctt
     4321 caagaagccg ctcgaacggg tgtttcgcga cttcatcgac gccggtctcg gatcgctccc
     4381 gggtggcggc gccgaggtgt tggtcgagcg ggtgcgacgc gagatctatc ccaagaagat
     4441 ctcggccgag cggtggctcg agatcgtccg attggcccat gagttcggcc tacggtcgaa
     4501 cgccacgctg ctttacggcc acgtcgagcg ggtcgacgaa cgcatcgagc acctgtgccg
     4561 gctacgcgcg ttgcaggacc ggaccggcgg gttcatgtgc ttcatcccgc tggcgttcca
     4621 cccggagaac accgagctga gccagctgcc gggtccgacc ggcttcgacg acttgatgac
     4681 gatcgcggtc tcgcgcttga tgctggacaa cttcgaccac gtcaaagcct actggatcat
     4741 gctgacacca cggatcgcgc aaaccgcgct caacatgggc gccgactgca tcgacggcac
     4801 ggtcgtcgag gagaagatcg tgcacatggc aggggccaag accccggtcg gactggaaat
     4861 ggatgagctg ttgcggctga tccgcgaggc gggacgcctg ccggtgcagc gcgattcgga
     4921 gtacaacgaa ctgcgacgtt tcaccgaaca tgccgtcgct tgag
//
LOCUS       DASPGN010000113        16526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_254981, whole genome shotgun sequence.
ACCESSION   DASPGN010000113 DASPGN010000000
VERSION     DASPGN010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 16526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16526
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254981"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1198
                     /locus_tag="VD788_03120"
     CDS             179..1198
                     /locus_tag="VD788_03120"
                     /inference="COORDINATES: protein motif:HMM:NF021497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HXV75283.1"
                     /translation="MVCLLGLIVAWPGAAAGQADPPRDYSGLLSLYVENDLFLRSDDN
                     YTSGVGVSWTSNDASAYRDRAFANRFVRAASFLPTIGLTRFRHFVAFTLGQEIYTPAD
                     ISSPDAPPDQQPYAGVLYLDSSVFSLGRRTMHAFTLRLGCVGPCSSADQIQREIHQWI
                     GSPVPAGWDDQLEDEFLLNLDYDWDWRIRRVTGPHKADYDFSLQAGAGFGNYYVGANG
                     GIVARVGHNLPDNYGAASFRSGGAAGVAGLAPPPGGRWGFYLFVGLQLSVVGRFLPTD
                     GNTFVDGPRIDRDDLFGNLSSGVTVGRGRVLFSWTINNVAGLTRFEQSRADDFGTLSF
                     SYYFK"
     gene            1262..2503
                     /locus_tag="VD788_03125"
     CDS             1262..2503
                     /locus_tag="VD788_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV75284.1"
                     /translation="MTTKRLRVAGLAFAALLALPPRPVAAEGIDWPQFRGADRDGVSS
                     ETGLLQTWPDGGPNELWRVELGEGYSAVSIAGDRVYTMYAAEHEGKPVEVAAAFEAAS
                     GKLLWKTAVGPKKETEFGNGPRSTPTVDGQTVYVLGSEGSLAALNAADGKPNWQVELT
                     TMFGGQVPFWGFSTSPLVDGELLILQAGGADGKSYVALDKKTGELKWAIGDGGAGYNS
                     PLLVELGGARRYLCFGGGKLMALDGQGAEVWSVDWPRGETHAMPVFIPPDRIFASGAE
                     GIGARLLRVGNDADGKVAVEELWSDRFMRNHFSSSVAIGATVYGFDNATLKAISLDDA
                     KLSWAKRGLGKGSLIYADGNLLVLSDRGELLLVRASPDAFTQHGAVQALDGRTWTAPS
                     LSRGRLYLRDQKQMVAYDLRQ"
     gene            2520..3707
                     /locus_tag="VD788_03130"
     CDS             2520..3707
                     /locus_tag="VD788_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75285.1"
                     /translation="MRGLGKLMLCFAVAAPTGGAVAATELDEIVAKHVEAKGGDRWKA
                     IETMKMSGSFTAFSIVAPFELIRKRDRKFSLDHEMNGHPVKIGFDDAVLWWDHAMLGP
                     GAKKIPDGPDRHAVEREIDFVTPLFDHAERGFELKLVGPTEFEGFPVIAIELTRPDDT
                     VETWYLDRETYLEFARESPGSDFGTPMTVRTFFDDFRRVEGVMIPFVVESQWYTRDRV
                     MRVEKVEINVPVDDAIFAMPAPLGMASLLPMIDDGWSVSTSIRQQPGAPWEESQRDTE
                     VVGRMRGALIQEAYTSPDGNEVVWSLAYDQFREAYRLIRITDQTNHLDVLVGKFDEQG
                     KLVLSNLETGTTWTGFGMTFHTRVTFHEIGATGFKVDTEISTDGGESWFLAAKQQYAR
                     TAD"
     gene            3804..5630
                     /gene="typA"
                     /locus_tag="VD788_03135"
     CDS             3804..5630
                     /gene="typA"
                     /locus_tag="VD788_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXV75286.1"
                     /translation="MSFRNVAIIAHVDHGKTTLVDGMLRQSGVFRSGRHVEDRVLDSM
                     DLERERGITILAKNTAIDFGAVRINIVDTPGHADFGGEVERSLHMVDGALLLVDASEG
                     PLPQTRFVLRKALEQRLPIILVVNKIDRRDARIQQVVNEVYDLFIDLDVDESQLDFPI
                     LYTDARRGIAHREPTDDSSSLLPLFETIVTTVPPAGGSSEAAPRFLVTNLDYDAYVGR
                     LALGRLFDGSLKMNRTYGLCREDGSVVPIRFSALYNYSGLERIETSEVQAGDICAFAG
                     VDQIHIGDSVTDLEHPRPLRRIHVDEPTVSMLFMVNNGPFAGRDGKLLTSRQIRDRLD
                     LERLGNVAIQIRESDRPDAFEVCGRGELQLGILIETMRREGFELLVSRPRVLRREIDG
                     KLHEPVEHLYLDVPEQFLGVVTEKLGMRKGRMINLANHGTGRVQVEFRIPSRGLIGFR
                     SEFLTDTKGSGVMNSLMNGWAEWAGPIPKRTSGTLVADRGGRASAYAMFGLEERGELF
                     VSPGSEVYEGMIVGERNRSGDLDVNVTREKKLTNMRSSNADVLETLRPPRTLSLDQSI
                     EFIDDDEYVEVTPASIRLRKGELSSSKRETARKRVAKQGVAR"
     gene            complement(5862..7172)
                     /locus_tag="VD788_03140"
     CDS             complement(5862..7172)
                     /locus_tag="VD788_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75287.1"
                     /translation="MDGERRTTTLGTIFRFWLPMAGTWLMMALEAPFLAAVIARLDDP
                     KENLAAFGVAYAIAILVESPVIMILSASTALVDDERSFRRLRRFTYALNATLTAGMTA
                     LLLSGGWRTTASMLIGLPAEVVELTQHALLALLPWPAAIGYRRFYQGLLIRNQQTRRV
                     ACGTVIRLGTMAGAALVLFAYSSLPGVLIAAFALSLGVCGEAAASRLMSVDAVRTHVR
                     GGRDGGAELSYLGIWRFYAPLALTSTISLAVHPLVAFFIGRAPFPLESLAVLPVVNSL
                     SFIFRSLGLSFQEVAIALLGRDRRNLDPVGRFAALLGAGSGLAMVSIAFTPLATAWFR
                     DLSGLTPELTRFAVPPARILTLLPPLSVLLSLQRAILVHGRDTAPITWATMLEVAGIV
                     LALTALAWGLGLTGVTAAALAFVAGRIAGNVYLVAPCRRLTRGP"
     gene            7274..8641
                     /locus_tag="VD788_03145"
     CDS             7274..8641
                     /locus_tag="VD788_03145"
                     /inference="COORDINATES: protein motif:HMM:NF023166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HXV75288.1"
                     /translation="MRRTVLRGALVAALALPSIGCDPGSPGGERLPERFRGKLLLEDG
                     RRSFVPCGTDRGVAVEDGTAGEIELAIEALASDPYRPLFVELRGVLGATGVLRVEELV
                     RAVREGRRCEEDVRGFLARGTGNEPSWSVVVAEGGIVFSQIGASLEFPFEPAIEQGAT
                     RVWATRLALPPHSLRFELVAERCADGMSGAWSGHRAEVEIDGRRYTGCGYEGWPMLEP
                     AVAYEIVTLDETLGGCGPSGEEACTWFRFDYARLDPVTGGGLSEAVNTEITAFVRAPL
                     VEDAPPADPAELVDRWNERYRAIKAEFPDSSQRWFVRRTAIPRSGPPMLLNVVLSESA
                     YTGGAHPNRRDDTLCFDRRSGRRLALDDLLEPGAIDALTALAEQRFRQTEQIPPDGGF
                     AEAGYWFDGDRFSLPADFALTAAGLEFQYDPYEIAPYAVGPTTLVLDLASIEPLLRPA
                     FRVVE"
     gene            complement(8723..9019)
                     /locus_tag="VD788_03150"
     CDS             complement(8723..9019)
                     /locus_tag="VD788_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXV75289.1"
                     /translation="MGSKLYVGNLPFSATEDEVRELFSQNNRTVNGVNLINDRDTGRP
                     RGFGFVEMGSDEEAQDAIRELNGHSLNGRPLTVNEARERGSGGGGGRRDRFSSY"
     gene            9358..11148
                     /locus_tag="VD788_03155"
     CDS             9358..11148
                     /locus_tag="VD788_03155"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HXV75290.1"
                     /translation="MGWEGWFTLGLVGLMGVALVRGMAGPDTTLLAGITVLMTMRLFS
                     TRFPRPVELVAGFGNEGLVTIAVLFVVASGLSRTGAMDMLTRPLLGRPRSVPRAQLRV
                     MAPVAALSAFINNTPIVAVFTPVIADWCRATRLQPGKLFIPLSYAAVLGGTCSLIGTS
                     TNVYVYGWLDAELKQRVGMFTIGCVGLPAAVIGIAYVLLVSRRLLPSGTTLDRQIGDV
                     REYTVEMLVEPNSAIDGKTIEQAGLRHLPGVYLIEIERGDEMLAAVGPRQTLFGGDRL
                     IFVGVVESVRDLQRIRGLVPATDQVFKLTGAHGNRVLVEAVVGSQFPALGRTVREGEF
                     RTRYDAAIIAVHRGGERIRKKIGEIVLRPGDTLLLETHPEFVKRYRDSNHFHLTSTLP
                     DSTPRRHDRAWVAVGILAAMILAVTIGVLGLLNAALIATGLMVVTRCTTPAEALEQVN
                     WRVLLAMGAAIGVGVSLESTGAASSIARLAIEGTRGLHPTTLLAAIYLLTLLFNVLVG
                     HAAAAALAFPVAQAAAVSTGVDFLPFVIVVMMAASADFANPVSYPTHLMVYGAGGYRF
                     GDFVRIGLPLNVLVMIVTVGLAPWIWPLHH"
     gene            11168..12025
                     /locus_tag="VD788_03160"
     CDS             11168..12025
                     /locus_tag="VD788_03160"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXV75291.1"
                     /translation="MRGDPRWIALGLGVAVLAGCAGDGGERYADAMAREHAGDRPVTS
                     GAAGGETAIAIDEQEVVYAEIDGVPISGVLARPAERPAELPGIIVIQEWWGLNDNIRQ
                     MTRKLAGLGYTALAVDLYRGQVAADPDEARRLMSEANQRGAESEQNLAQAREYLARQG
                     ATRVGVIGWCFGGGWSLRAALAMPDRLDAAVIYYGQLVTDAERLAGLETPVLGIFGAE
                     DGGIPLDTVREFERVLAELGKPASIHVYPGADHAFANPSGTRYDAEAAADAWDETVAF
                     FARQLKSAE"
     gene            12022..12507
                     /locus_tag="VD788_03165"
     CDS             12022..12507
                     /locus_tag="VD788_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HXV75292.1"
                     /translation="MTPERDRDERGRPRFAGPRDRLGRPLPRGRRDQLAGRGDPARDD
                     PTPAVALAEAARLFDAERYFEAHEYFEYLWKHPAVDDGHRSTWRAAAQLAVGLCHGQR
                     GNRRGASRVLRRASHALDRIDQAPPGVDRHALAALARAACERVDRGEPSDGRLPRFPR
                     R"
     gene            complement(12504..13139)
                     /locus_tag="VD788_03170"
     CDS             complement(12504..13139)
                     /locus_tag="VD788_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75293.1"
                     /translation="MDVTQQRRYRAGDKVDDLCRACKIVRVHSVFVAGEQGEPIRVVC
                     DYCGSQHNYRGRTTMPAAGGEAASATVRSPFAVVSERERSRPPMGLEGAGAEGRDLEM
                     LLRRIIREEAGVTPVVPADKWRGGELLLKPGRDELQPKSIPIETFFNKIVMIRNRLRV
                     LEQQINATELPLETKVKLQSYVTGCYGSLTSFNVLFAEEDDRFHGTGGRGD"
     gene            complement(13200..16388)
                     /locus_tag="VD788_03175"
     CDS             complement(13200..16388)
                     /locus_tag="VD788_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV75294.1"
                     /translation="MKLIDFSTRRPVSIFIYSVAAVVFGVVAFTNLATDLLPDITYPS
                     ITVRTDYDGAAPLEIENLITRPIENAVGVVNNVVRVVSSSRADTSEVTLEFAWGTDMD
                     LAALDVRERLDVLRLPVDAERPLLLRYDPSLDPIMRLGAYGEEDLVRLRLIAEEQIQR
                     ELERIEGVAAVVISGGLEEEILVELDERRIANLGLSEAIVLDRLSAENVDLTGGRLRE
                     GQTEYLVRTINEYVRPEDMRSIVVDASQGAIVRLEDVATVRKGHKEREVVTRIGGHES
                     VEVAVYKEGGTNTVAVSDAVKAQLAELGRRLRQIDPALELRVITDQAGYIRQSVSQVL
                     ETAVYGGFLAILMLFLFLRSWKTTLIVGVAIPISVVTTFFLMYVSGISLNIMSLGGLT
                     LGIGMLVDNSIVVLEAIQRKRDDGLGELQAAEAGADEVASAVVASTLTTICVFVPIVF
                     VEGIAGQLFGDQALTVTYSLSVSLIVALTLIPMLASRSFRPADPDADDERAARGAIGR
                     ALFGAAVALARITGGLARGLAAVTSALSRVPLRLFHAGYDRLAESYRRSLDAVLAHPV
                     PTVAVACALLFATLALYPRLGKELVPELVQGEFFVDIQLPPGTRLEVTERRIAALERF
                     ALAELFGVDALYAIAGTSNRQGGAAGERRENIGQLTVTLVPPVDRAREDAMIERLRQA
                     LEREPAIEYRFGRPSYFSFRTPIEVEIRGYNLTLLDRLSRELVRRMGGIDGLSDIESS
                     TEGGNPELRIRFDRERLASLGLSVSDVAGTVRTKVQGTVATDIQREDRQIDIRMRSAE
                     TFRDSVDDLLALTVYQRDKTAVPLSAVADVEEVEGPAEIRRVEGERVAVVSANLVGRD
                     LESVSREIGAALESMQLPGGYEWRIGGQRQEMQTSFGSMRLAIGLAIFMVYLVMASQF
                     ESLLHPFVILFSVPLALIGVLLTLWAFRINISIVVLIGVILLAGIVVNNAIILLDHAN
                     RLRRRDGLSKREALRRAGRVRLRPILMTTSTTVLGLLPMAVGIGAGSELRRPMALVVI
                     GGLLTSTALTLLVVPVVYDLLDRRRRLAPSSG"
BASE COUNT         2631 a         5553 c         5752 g         2590 t
ORIGIN      
        1 gttggggcgt gccgctggcc gaggcgaatc gggagctgat cgcgcggctc gtcgacggcg
       61 gtctggtgcg ggccgagggt ctccggctgc gaccgacgct ggcaggtctc gctgtcgccg
      121 actcgatcgc cgcggagttc gtcgtctgag cccgtcggac acagggtcgc ggatcgccgt
      181 ggtctgcctc cttggcctga tcgtcgcctg gccgggcgca gcggcgggcc aggcggaccc
      241 gccgcgcgac tactccgggc tgttgtcgct atacgtcgag aacgacctgt tcttgcgcag
      301 cgacgacaac tacacgtcgg gcgtgggcgt cagttggacc tcgaacgacg cctcggccta
      361 tcgcgaccgc gcgtttgcca accgcttcgt ccgcgccgcg tcgttcctgc cgacgatcgg
      421 cctcacgcgg tttcgccatt tcgtcgcgtt cacgctcggc caggagatct acacccccgc
      481 cgacatcagc tcgccggacg caccgcccga ccagcagccc tacgccggcg tgctgtacct
      541 cgactcgtcc gtcttttcgc tcggccggcg cacgatgcac gccttcacgc tgcgtctcgg
      601 ttgcgtcggc ccgtgctcgt cggccgacca gatccagcgc gagatccacc agtggatcgg
      661 ctcgccggtg ccggccggct gggacgacca gctcgaggac gagttcctgc tgaatctcga
      721 ctacgactgg gactggcgga tccgtcgcgt caccggcccg cacaaggccg attacgactt
      781 ctcgctgcag gccggtgccg gattcggcaa ctactatgtc ggggccaacg gcgggatcgt
      841 ggcccgcgtc ggccacaacc tgccggacaa ctacggggcg gcgagcttcc gcagcggagg
      901 cgcggcgggg gtcgccgggc tggcgccgcc ccccggcggt cgctggggtt tctacctgtt
      961 cgtcgggctg cagctgtcgg tggtcggtcg cttcctgccg accgacggca acaccttcgt
     1021 cgacgggccg cgcatcgatc gggacgatct gttcggcaac ctgtcgagcg gcgtcaccgt
     1081 cggtcgcggc cgggtgttgt tttcgtggac gatcaacaac gttgccggac tgacccgctt
     1141 cgaacaatcg cgcgccgacg acttcggcac cttgtcgttc tcgtactact tcaagtaggg
     1201 agcgaagccc gaccatcctg tcgtagaatg gcggcttcgt ttgccagaga gggaggcgtg
     1261 tatgacgacg aagcgactcc gggtcgccgg attggcgttt gcggcgctgc tggcgttgcc
     1321 gccgcgaccg gtcgcggccg aggggatcga ttggccgcag tttcgcggcg cggaccgcga
     1381 cggggtgtcg agcgagaccg gattgctcca gacctggccc gacggcggcc cgaacgagct
     1441 gtggcgcgtc gagctgggcg agggctattc ggcggtctcg atcgccggcg accgagtgta
     1501 cacgatgtac gccgccgagc acgagggcaa accggtcgag gtcgcggccg cgttcgaggc
     1561 ggcgagcgga aagctgctgt ggaagacggc ggtcggcccg aagaaagaga ccgagttcgg
     1621 caacggaccc cggtcgacgc cgaccgtcga cggccagacg gtctacgtgc tggggtccga
     1681 gggcagtctc gccgcgctga acgcggcgga cggcaagccg aactggcaag tcgagctgac
     1741 cacgatgttc ggtgggcagg tgccgttctg gggtttttcc acctcgccgt tggtcgacgg
     1801 cgagctgctg atcctgcagg ccggcggcgc cgacggcaag tcctacgtcg cgctcgacaa
     1861 gaagaccggc gagctgaaat gggcgatcgg cgacggcggc gccggctaca actcgccgtt
     1921 gctcgtcgag ctcggcggcg cacggcgcta cctctgcttc ggcggcggca agctgatggc
     1981 tctcgacggg cagggagcgg aggtctggtc cgtcgactgg ccgcgcggcg agacgcacgc
     2041 gatgccggtg ttcatccccc ccgaccgcat cttcgcctcg ggcgccgagg gcatcggcgc
     2101 gcggctgctg cgtgtcggca acgacgccga cggcaaggtc gcggtcgagg agttgtggtc
     2161 cgaccgcttc atgcgcaacc acttcagctc ctcggtcgcg atcggcgcca cggtctacgg
     2221 tttcgataac gcgacgttga aggcgatctc gctcgacgac gcgaagctgt cgtgggccaa
     2281 gcgcgggctg ggcaagggtt cgttgatcta cgccgacggc aacctgctgg tgctgtccga
     2341 ccgcggcgag ctgttgttgg ttcgggcgag ccccgacgcc ttcacgcagc acggcgcggt
     2401 ccaggcgctc gacggccgaa cctggacggc gccgtcgctg tcgcgcggcc ggctctacct
     2461 gcgcgaccaa aaacagatgg tggcctacga cctgaggcag tgaggaggag gggaaaccga
     2521 tgcgtgggct gggcaagctg atgctgtgtt tcgcggtggc ggcgccgacg ggcggcgccg
     2581 tcgcggcgac cgagctcgac gagatcgtgg ccaagcacgt cgaggccaag ggcggcgatc
     2641 gctggaaggc gatcgagacg atgaagatga gcggctcgtt caccgcgttc agcatcgtgg
     2701 cgccgttcga gctgatccgc aagcgcgacc gcaagttctc gctcgatcac gagatgaacg
     2761 gccatccggt caagatcggt ttcgacgacg cggtgttgtg gtgggatcac gcgatgctgg
     2821 gtcccggagc gaagaaaatc cccgacggtc ccgaccggca cgcggtcgag cgggagatcg
     2881 acttcgtgac gccgctgttc gaccacgccg agcggggttt cgagctgaag ttggtcggac
     2941 cgaccgagtt cgaaggcttt cccgtcatcg cgatcgagct gacgcggccg gacgacacgg
     3001 tcgagacgtg gtatctcgac cgcgagacct acctcgagtt cgcacgcgaa tcgccgggct
     3061 ccgatttcgg cacgccgatg acggtccgga cgttcttcga cgacttccgc cgggtcgagg
     3121 gcgtgatgat cccgttcgtc gtcgagagcc aatggtacac gcgcgaccgg gtgatgcgcg
     3181 tcgaaaaggt cgagatcaac gtgcccgtcg acgacgcgat cttcgcgatg ccggccccgc
     3241 tggggatggc ctcgttgctg ccgatgatcg acgacggctg gagcgtctcg acgtcgatcc
     3301 ggcagcagcc gggcgcgccg tgggaagagt cgcagcgcga taccgaggtc gtcggccgga
     3361 tgcgcggggc gctgatccag gaagcctaca cctcgccgga cggcaacgag gtcgtctggt
     3421 cgctggccta cgaccagttc cgcgaggcgt atcggttgat ccgcatcacc gaccagacga
     3481 accatctcga cgtgctggtg ggcaagttcg acgaacaggg caagctcgtg ttgtccaatc
     3541 tggagaccgg cacgacgtgg acgggtttcg gcatgacctt ccacacccgc gtcacctttc
     3601 acgagatcgg agcgaccggg ttcaaggtcg acacggagat ctcgaccgac ggcggagaga
     3661 gctggttcct cgcggccaaa cagcagtacg cgcggacagc agactgaccg ttccccggcg
     3721 gccgcggccg cccggcgggt ggagaaagcc acggctttct ggtatcacga cgccggtgcg
     3781 gtcgcatccg ctcggggaat tccatgtcgt tccgcaacgt cgccatcatc gcccacgtcg
     3841 accatggcaa gaccacgctg gtcgacggca tgctgcggca gagcggcgtg ttccgcagcg
     3901 ggcgtcacgt cgaagaccgc gtcctggaca gcatggatct cgaacgcgag cgcgggatca
     3961 ccatcctcgc caaaaacacc gcgatcgact tcggtgcggt gcggatcaac atcgtcgata
     4021 cgccgggaca cgccgatttc ggcggtgagg tcgaacgcag cctgcacatg gtcgacggcg
     4081 ccctgctgct ggtcgacgcc agcgaagggc cgctgccgca gacgcgattc gtgttgcgca
     4141 aggcgctcga gcagcgcctg ccgatcattc tggtggtcaa caagatcgat cgcagggacg
     4201 ctcggatcca gcaggtggtc aacgaggtct acgacctgtt catcgacctc gacgtcgacg
     4261 agtcacagct cgacttcccg atcctctaca ccgacgcgcg tcggggcatc gcccaccgcg
     4321 aaccgaccga cgactcgtcg agcctgctgc cgttgttcga gacgatcgtc accacggtgc
     4381 cgccggccgg cggctcgtcc gaggcggcac cccgctttct cgtaaccaat ctcgattacg
     4441 acgcctacgt cggacggctg gccctcggtc ggctgttcga cggaagtctg aagatgaacc
     4501 gcacctacgg tctgtgccgc gaagacggta gcgtcgtgcc gatccgcttc tcggcactct
     4561 acaactactc cggtctcgag cggatcgaga cgtcggaggt tcaggccggc gacatctgcg
     4621 ccttcgccgg tgtcgaccag atccacatcg gcgacagcgt gaccgacttg gagcacccgc
     4681 gaccgctgcg gcggatccac gtcgacgagc cgacggtgtc gatgctgttt atggtcaaca
     4741 acggcccgtt cgccggccgc gatggcaagc tgctcacgtc gcgccagatc cgcgatcggc
     4801 tcgacctcga acggttgggc aacgtcgcaa tccagatccg cgagtccgat cggcccgacg
     4861 cgttcgaggt gtgcggccgc ggcgagctcc agctcggtat cttgatcgag acgatgcggc
     4921 gcgaggggtt cgagctgttg gtctcgcggc cccgcgtgct gcgacgcgag atcgacggca
     4981 agctgcacga gccggtcgag cacctgtatc tcgacgttcc ggagcagttc ctcggcgtcg
     5041 tgacggaaaa gctcggtatg cgcaaggggc gaatgatcaa cctggccaat cacggaaccg
     5101 ggcgggtgca ggtcgagttc cgcatcccgt cgcggggcct gatcggcttc cgcagcgagt
     5161 tcctcaccga caccaagggc tcgggcgtga tgaactcgct gatgaacggc tgggccgagt
     5221 gggccgggcc gatccccaag cgaacgagcg ggacgctcgt cgccgaccgc ggcggacgag
     5281 cgtcggccta cgcgatgttc gggctggagg agcgcggcga gttgttcgtc agcccgggat
     5341 ccgaggtcta cgaggggatg atcgtcggcg agcggaatcg ctcgggcgat ctcgacgtca
     5401 acgttacgcg tgagaagaag ctgaccaaca tgcgcagcag caacgcggat gtgctcgaga
     5461 cgctgcgtcc gccgcgtacg ctttcgctcg accagtcgat cgagttcatc gacgatgacg
     5521 agtacgtcga ggtcaccccg gcgtcgatcc gcctgcgcaa gggcgagctg tcgtcctcca
     5581 aacgcgagac cgcgcgcaaa cgggtggcga agcagggcgt cgcccggtga ggcggcgggc
     5641 cggggggctg gccgatcaac cgtcgagccc gacccgacgg aaggcggccg agccccccta
     5701 ccgcacgtcc cgttcgacga tctcgccgtg tccgacgacc agccggtcga cgacgcagcg
     5761 ggtcgttgca cgcgtacaaa ccgcgagcga cctcgatcaa accgctgctc cccgtctcca
     5821 cgatcgccgg tcagtataga cggccgcggc cgggcgccgg ctcacgggcc gcgcgtcaga
     5881 cggcgacaag gcgcgacgag gtagacgtta ccggcgatcc gtccggcgac gaacgcgagc
     5941 gcggccgccg tgacgcccgt caggcccagg ccccaggcca gggcggtcaa cgccagcacg
     6001 atgccggcga cctcgagcat ggtggcccag gtgatcggtg ccgtgtcgcg gccgtgcacc
     6061 agaatcgcgc gttgcagcga caacagcacc gacagcggcg gcagcagcgt gaggatccgc
     6121 gccggcggaa cggcgaatcg cgtcagctcg ggcgtcaatc cggagaggtc gcggaaccac
     6181 gcggtcgcga gcggcgtgaa ggcgatcgac accatcgcca gcccgctgcc ggcgccgagc
     6241 agggcggcga acctgccgac gggatcgagg ttgcgccggt cccgaccgag caacgcgatc
     6301 gcgacctcct gaaacgacag accgaggctg cggaagatga acgacagcga gttgacgacc
     6361 ggcagcacgg cgagagactc gagtgggaac ggcgcgcggc cgatgaagaa cgcgaccagc
     6421 gggtggaccg cgagcgagat cgtcgacgtc agtgcgagcg gtgcgtagaa gcgccagatg
     6481 ccgaggtagc tcaattcggc gccgccatcc cgcccgccgc gcacgtgagt gcgaaccgca
     6541 tcgaccgaca tcaaccgact ggcagccgct tcgccacaga cgccgagcga taatgcgaag
     6601 gcggcgatga gcacgccggg caacgacgag tacgcgaaca ggacgagcgc ggccccggcc
     6661 atcgtaccca gtcggatcac cgtgccgcag gcgacccgcc gggtttgttg gttgcggatc
     6721 aacagaccct gataaaaccg gcggtagccg atcgccgccg gccacggcag gagcgccagc
     6781 aacgcatgct gggtcagctc gacgacctct gccggaagtc cgatcagcat cgacgcggtc
     6841 gtgcgccacc cgccgctcaa cagcaacgcg gtcatacctg cggtcagcgt cgcgttgagc
     6901 gcgtaggtga agcgtcgcag ccggcgaaac gatcgttcgt cgtcgactag ggcggtcgac
     6961 gcgctgagga tcatgatcac cggcgactcg acgaggatcg cgatcgcgta ggcgacaccg
     7021 aacgcggcca ggttctcctt cggatcgtcg agtcgcgcga tcacggcggc gaggaacggc
     7081 gcctcgagcg ccatcatcag ccaggttccc gccatcggca gccagaagcg gaagatggtg
     7141 ccgagggtcg tcgttcgtcg ttcgccgtcc atcgaagcgg cgattctagc gagtcgtccc
     7201 gttctcgtcc gcttggtcgc gcggcacacg gcgtgcgaga atggagcccg ctcggtcggt
     7261 ggtaggggga accgtgcgaa gaaccgtcct tcgcggtgcg ctggtcgcgg cgctggcact
     7321 accgtcgatc ggttgcgacc ccggttcacc cggcggtgaa cggttgcccg agcggtttcg
     7381 cggcaagctg ctgctcgagg acggacgacg gtcgttcgtc ccgtgcggga cggatcgcgg
     7441 ggtcgcggtc gaagacggca ccgcaggcga aatcgagttg gcgatcgagg cgctggcgag
     7501 cgatccgtat cgaccgctgt tcgtcgagct gcgcggggtg ctgggtgcga ccggggtgct
     7561 gcgggtcgag gagctcgttc gtgcggtccg cgagggccgg cgctgcgagg aggacgtgcg
     7621 gggctttctc gcgcgcggga cgggcaacga gccgtcgtgg agcgtcgtcg tcgcggaagg
     7681 cggcatcgtc ttttcgcaga tcggcgcgtc gctcgaattc ccgttcgagc cggcgatcga
     7741 gcagggcgcg acgcgcgtct gggccacgcg gctcgcgctc cccccgcact cgcttcggtt
     7801 cgagctcgtc gcggagcgct gcgccgacgg catgtcgggg gcgtggtccg gtcatcgcgc
     7861 cgaggtggaa atcgacgggc ggcgttacac cggctgcggg tacgagggtt ggccgatgct
     7921 cgagccggcc gtggcatacg agatcgtaac cctcgacgag acgctcggcg gctgcgggcc
     7981 gagcggagaa gaggcctgca cctggttccg cttcgactac gcgcggctcg atccggtgac
     8041 gggcggcggg ttgtccgagg cggtcaacac cgagatcacg gcgttcgtcc gcgcgccgct
     8101 ggtcgaggac gcgccgccgg ccgatccggc cgagctcgtc gaccgctgga acgagcggta
     8161 ccgcgcgatc aaggccgagt ttcccgactc gtcgcagcgc tggttcgtcc ggcgcacggc
     8221 gataccgcgg tcgggcccgc cgatgctgct caacgtcgtg ctgagcgaat cggcctacac
     8281 cggcggggcg catccgaatc gccgcgacga cactctgtgt ttcgaccggc ggagcgggcg
     8341 ccgcctcgcg ctcgacgatc tgctcgagcc gggagcgatc gacgcgttga ccgcgctggc
     8401 ggagcagcgc ttccgccaga ccgagcagat cccgccggat ggcgggttcg ccgaagccgg
     8461 ctactggttc gacggcgacc ggttctcgct cccggcggat ttcgcgctga ccgcggccgg
     8521 tctcgagttc caatacgacc cgtacgagat cgcaccgtac gccgtcgggc cgacgacgct
     8581 cgtgctcgac ctcgcgtcga tcgagccgct gctgcgtccc gcgttccgcg tcgtggaatg
     8641 agacgcgcga aaaagaatcg gcccgcccgc caagcgagtc ggcgggcggg ccgtcgtgag
     8701 cctcgagccc gagatgcctc gatcagtaac tcgagaagcg gtcgcgacgg ccgccgccac
     8761 cgccggatcc gcgctcgcgg gcctcgttga cggtcagcgg tcgaccattc aacgaatgac
     8821 cgttgagctc gcggatcgcg tcctgcgcct cctcgtcgct tcccatctcg acgaagccga
     8881 aaccacgcgg tcgcccggtg tcccggtcgt tgatgagatt gacgccgttc accgtacggt
     8941 tgttctgcga gaacaactcg cgaacctcgt cctcggtggc gctgaacggc aaattgccga
     9001 cgtagagctt gctacccatt cctcttgact cctaaccaat tgccgacgcg ctgccgaccg
     9061 agacctggtt gactcgatcc gacccgtcga cccccaatac gcatgacgga tcacggacga
     9121 cgcggatgca atccagacag aactcccgtg cccgacggag cgtcaggtag gtcgatctcg
     9181 actcagcaac cacttggccc ggaaaccaaa acaaacctcg gcccaagtct acacgaaaac
     9241 cgaagccgat gacgcgggtt tcgccggtgg atagactgtc gggtcggacc ggccggcgaa
     9301 aggtccagcg cgacgaacgg gttgcggtgc gttccggccg ggtccccggg ggaacgggtg
     9361 ggctgggagg gttggttcac gctcggactg gtcgggttga tgggcgtcgc gctggtgcgg
     9421 gggatggccg gcccggacac gacgctgctc gccgggatca ccgtcctgat gacgatgcgg
     9481 ctgttctcga cccgcttccc ccggccggtc gagctcgtcg ccggcttcgg caacgagggg
     9541 ttggtcacga tcgcggtgct gttcgtcgtc gcctccgggc tgtcgcggac cggcgcgatg
     9601 gacatgctga cccgacccct gctcggccgg ccgcggagcg ttccacgcgc gcagctgcgg
     9661 gtgatggcgc cggtcgccgc gctcagcgcc ttcatcaaca acaccccgat cgtcgccgtc
     9721 ttcaccccgg tgatcgccga ctggtgccgg gccacacgtc tccagccggg caagttgttc
     9781 attccgttgt cctatgccgc ggtgctcggc gggacgtgct cgttgatcgg aaccagcacc
     9841 aacgtctacg tctacggttg gctcgacgcc gagctgaagc agcgcgtcgg gatgttcacc
     9901 atcggctgcg tcggcttgcc ggccgcggtg atcgggatcg cctacgtgct cctcgtctcg
     9961 cgacggctgc ttccgtccgg tacgacgctc gacaggcaga tcggcgacgt gcgcgagtac
    10021 acggtcgaga tgctggtcga gccgaacagc gcgatcgacg ggaagacgat cgagcaagcg
    10081 ggactgcgtc atcttcccgg ggtctatctg atcgagatcg agcgcggcga cgagatgctg
    10141 gccgccgtcg gaccgcggca gacgctcttc ggcggcgatc gtctgatatt cgtcggcgtc
    10201 gtcgagtcgg tgcgcgacct gcaacggatc cgcggtttgg tgccagcgac cgaccaggtg
    10261 ttcaagctga ccggtgcgca cggcaaccgc gtgctcgtcg aggccgtcgt cggcagccag
    10321 ttcccggcgc tggggcgcac ggtgcgcgag ggagagttcc gcacccgcta cgacgcggcg
    10381 atcatcgccg tccaccgcgg cggggagcgg atccgcaaga agatcggtga gatcgtcctg
    10441 cggcccggcg acacgctgtt gctcgagacc catccggagt tcgtcaagcg gtaccgcgac
    10501 agcaaccact tccacttgac ctcgaccctg cccgactcga cgccgcggcg ccacgatcgc
    10561 gcctgggtcg cggtcggcat tctcgccgcg atgatcctcg ccgtgacgat cggcgtgctc
    10621 ggcctgctca acgccgcgct gatcgcgacc ggtttgatgg tcgtgacgcg ctgcacgacg
    10681 ccggccgagg cgctggaaca ggtcaactgg cgggtcctgc tcgcgatggg tgccgcgatc
    10741 ggcgtcggcg tctcgttgga gtcgaccggc gccgcgagct cgatcgcgcg gttggcgatc
    10801 gaaggcacgc gcggcctgca cccgacgacg ctgttggcgg cgatctacct gctgacgttg
    10861 ctgttcaacg tgctcgtcgg ccacgccgcg gccgccgcgc tggccttccc ggtggcgcag
    10921 gcggccgcgg tctcgaccgg ggtcgatttc ctgccgttcg tcatcgtcgt gatgatggcg
    10981 gcgtcggccg atttcgccaa tcccgtaagc tacccgacgc acctgatggt ctacggggcc
    11041 ggcggctatc gtttcggcga tttcgtccgc atcgggctcc cgctgaacgt cctggtgatg
    11101 atcgtgaccg tcggtctggc gccgtggatc tggccgcttc accattgatc gagcgaggag
    11161 ggattccatg agaggcgacc cgagatggat cgcattgggt ttgggagtcg cggtgctcgc
    11221 gggctgtgcc ggcgacggcg gcgagcgcta cgccgacgcc atggcacgcg agcacgccgg
    11281 cgatcgaccg gtgacgagcg gcgccgccgg cggggagacg gcgatcgcga tcgacgagca
    11341 ggaagtcgtc tacgccgaga tcgacggagt gccgatcagc ggggttctcg cgcgcccggc
    11401 cgagcgcccg gctgagctac ccgggatcat cgtgatccag gagtggtggg gcttgaacga
    11461 caacatccgg cagatgacac gcaagctggc cggcctcggc tatacggcgc tggccgtcga
    11521 cctctatcgc ggccaggtcg ccgccgatcc ggacgaggcg cgccggctga tgagcgaggc
    11581 caaccagcgg ggggcggagt cggagcagaa cctggcccag gcccgcgagt acctcgcgcg
    11641 acagggagcg acgcgggtcg gcgtgatcgg ctggtgcttc ggcggtggct ggtcgctgag
    11701 ggcggcgctc gcgatgccgg accgtctcga cgccgcggtg atctactatg gccagttggt
    11761 gaccgatgcg gagcggctcg ccggtctcga gacaccggtt ctcggcatct tcggcgcgga
    11821 agacgggggc attccgctcg acacggttcg cgaattcgaa cgagtgctcg ctgagctggg
    11881 caagccggcg tcgatccacg tctacccggg tgccgatcac gccttcgcca acccgtccgg
    11941 cacccgctac gatgccgaag cggcagccga cgcgtgggac gagacggtcg cgttcttcgc
    12001 acggcagctc aagtccgccg agtgacgccg gagcgcgatc gcgacgagcg cggccggccg
    12061 cgattcgccg ggccccgcga tcgtctgggg cgcccgctcc cgcgcggtcg gcgagaccaa
    12121 ctggccggtc gcggcgaccc ggcgcgggac gacccgacgc ccgccgtggc gctggccgag
    12181 gcggcgcggc tgttcgacgc cgagcgctac ttcgaagccc acgagtactt cgagtacctg
    12241 tggaagcacc cggcggtcga cgacggccac cggtcgacgt ggcgcgccgc ggcccaactg
    12301 gcggtcgggc tgtgtcacgg gcagcgcggc aatcggcgcg gggcttcgcg cgtgctgcgg
    12361 cgcgcgtcac acgcgctcga caggatcgac caggcgccgc cgggcgtcga ccggcacgcg
    12421 ctggccgcgc tcgcgcgcgc ggcgtgcgag cgcgtcgatc gtggcgagcc gagcgacggg
    12481 cggttgccgc ggttcccccg tcgctagtcg ccgcggccgc ccgtgccgtg gaagcggtcg
    12541 tcttcctcgg cgaacagcac gttgaagctg gtcagcgagc cgtaacagcc ggtaacgtag
    12601 ctctgcagct tcactttcgt ctcgagcggc agttcggtcg cgttgatctg ctgctcgagc
    12661 acgcgcaggc ggttgcggat catcacgatc ttgttgaaga aggtctcgat cgggatcgac
    12721 ttcggctgca gctcgtctcg gccgggtttg agcagcaact cgccgccgcg ccacttgtcc
    12781 gccggcacga ccggggtcac gcccgcttcc tcgcggatga tcctgcgcag caacatttcg
    12841 agatcccggc cctcggcgcc ggcgccttcg aggcccatcg gtggtcggct cctttcacgt
    12901 tcgctgacga cggcgaacgg ggatcgaacg gtcgcgctcg ccgcctcgcc gcccgcggcc
    12961 ggcatcgtcg tgcggccgcg atagttgtgc tggctgccgc agtagtcgca gacgacgcgg
    13021 atcggctcgc cctgctcccc ggcgacgaag acggaatgga cgcgcacgat cttgcacgcg
    13081 cggcacaggt cgtcgacctt gtcccccgca cggtaacgcc gctgctgcgt gacgtccaat
    13141 ctccggttcc tgccgcgcta cgacgcgtga tgatagcaga acgcgcggtc cgcgcgggct
    13201 cagccggacg acggcgccaa acgccgccgc cggtcgagca ggtcgtagac caccgggacg
    13261 acgagcaggg tcagtgcggt cgaggtcagc agcccgccga tgacgaccaa cgccatcggc
    13321 cggcgcagct cgctgcccgc gccgatgccg accgccatcg gcagcaggcc gagcaccgtg
    13381 gtcgacgtcg tcatcaggat cggccgcagc cgcacgcggc ccgcgcgccg cagtgcctcc
    13441 cgcttcgaca gcccgtcgcg ccggcgcagc cgattggcgt ggtccagcag aatgatcgcg
    13501 ttattgacga cgatgcccgc caacaggatg acgccgatca gcacgacgat gctgatgttg
    13561 atgcggaacg cccacagcgt caacagcacg ccgatcagcg cgagcggcac gctgaacagg
    13621 atgacgaacg ggtgcagcag cgactcgaac tggctcgcca tcaccaggta gaccatgaag
    13681 atggcgagac cgatcgcgag ccgcatgctg ccgaacgagg tctgcatctc ttgacgctgg
    13741 ccaccgatcc gccattcgta tccgccggga agctgcatcg actcgagcgc ggcgccgatt
    13801 tcgcgcgaca ccgactcgag atcgcggccg accaggttgg ccgataccac ggcgacgcgt
    13861 tcgccctcga cgcgccgtat ctctgccggc ccctcgacct cctccacgtc ggcgaccgcc
    13921 gacagcggca ccgcggtctt gtcgcgctgg tagacggtca gcgcgagcag gtcgtcgacg
    13981 ctgtcgcgga acgtctccgc ggagcgcatg cggatgtcga tctggcgatc ctcgcgttgg
    14041 atgtcggtgg caacggtgcc ttgcaccttg gttcgcaccg tgccggcgac gtcgctgacc
    14101 gacaggccga gcgacgccag tcgctcgcga tcgaagcgga tccgtagctc ggggttgcca
    14161 ccttcggtcg acgactcgat gtcgctcagt ccgtcgatgc cgcccatccg ccggaccagc
    14221 tcgcgcgaca gccgatcgag caacgtcagg ttgtagccgc ggatctcgac ctcgatcggc
    14281 gtgcggaagc tgaagtagct cggacggccg aagcggtact cgatcgccgg ctcgcgctcg
    14341 agcgcctgcc gcagtcgctc gatcatcgcg tcctcgcgcg cccgatcgac gggcggaacc
    14401 agcgtgaccg tcaattggcc gatgttctct cggcgttccc ccgccgctcc gccctgccgg
    14461 ttcgacgtgc cggcaatggc atacagcgcg tcgacgccga acagctcggc cagcgcgaat
    14521 cgttcgagtg cggcgatccg gcgctcggtc acctcgagcc gggtgccggg cggtagctgg
    14581 atgtcgacga agaactcgcc ctgcacgagc tccggcacca gttccttgcc cagccggggg
    14641 tagagcgcga gcgtggcgaa cagcagggcg cacgccacgg cgacggtcgg cacggggtgt
    14701 gccagcacgg cgtcgagcga gcgccgatac gattcggcga gccggtcgta accggcgtgg
    14761 aacagccgaa gcggaacgcg actgagcgcg ctggtaacgg cggccagacc gcgggccagc
    14821 ccgccggtga tccgcgccaa cgcgaccgcc gcgccgaaca acgcccggcc gatcgcgccc
    14881 cgggccgccc gctcgtcgtc cgcgtcggga tcggcaggac ggaacgagcg cgaagcgagc
    14941 atcgggatca gcgtcagcgc gacgatcaac gacacgctca acgagtaggt gaccgtcaac
    15001 gcctggtcgc cgaacagctg tccggcgata ccctcgacga agacgatcgg gacgaacacg
    15061 cagatcgtgg tcaacgtgct ggcgacgacg gcgctggcga cctcgtcggc gccggcctcg
    15121 gcggcttgga gctcgcccag gccgtcatcg cgcttgcgct gaatcgcttc gagcacgacg
    15181 atcgagttgt cgaccagcat tccgattccg agcgtcaggc cgcccaacga catgatgttc
    15241 agcgagatgc ccgaaacgta catcaggaag aaggtcgtca cgaccgagat cgggatggcc
    15301 acgccgacga tcagcgtcgt cttccagctg cgcaggaaga ggaacagcat cagaatcgcg
    15361 agaaaacccc cgtacaccgc cgtctcgagc acctgcgaca ccgattggcg gatgtagccg
    15421 gcctgatcgg tgatcacccg cagctcgagc gccgggtcga tctgtcgcag gcgccggccc
    15481 agctccgcca gttgcgcctt gaccgcgtcg gacacggcga cggtattggt gccgccttct
    15541 ttgtagaccg cgacctcgac gctctcgtgg ccgccgattc tggtgacgac ctcgcgctcc
    15601 ttgtgaccct tgcgcacggt cgccacgtcc tccagccgca cgatcgcgcc ctgcgaggcg
    15661 tcgaccacga tcgagcgcat gtcctccggg cgcacgtact cgttgatcgt gcggacgagg
    15721 tactccgtct gcccctcgcg cagacgcccg ccggtcagat cgacgttctc cgcggacagc
    15781 cggtcgagca cgatcgcttc gctcaggccg agattcgcga tccgccgctc gtcgagctcg
    15841 acgagaatct cctcctcgag gccgccactg atcacgaccg cggcaacccc ctcgatccgc
    15901 tccagctcgc gttggatctg ctcctcggcg atcagccgca accgcaccag gtcttcctcg
    15961 ccgtaggccc ccaggcgcat gatcggatcg agcgacgggt cgtagcgcag cagcaaaggt
    16021 cgctccgcgt cgaccggcag ccgcagcacg tcgagtcgtt cgcgcacgtc gagcgcggca
    16081 aggtccatgt cggtgcccca ggcgaactcg agcgtgactt cgctcgtatc ggcgcgcgac
    16141 gacgagacga cccgaacgac gttgttgacc acgccgaccg cgttctcgat cggccgcgtg
    16201 atcaggttct cgatctcgag cggcgccgcc ccgtcgtagt cggtgcggac ggtgatcgac
    16261 gggtaggtga tgtcgggcag caggtcggtc gccaggttgg tgaaggcgac gacgccgaac
    16321 acgacggcgg cgacggagta aatgaagatg ctgaccggtc gtcgcgtcga gaaatcgatc
    16381 aacttcaccg ggcgctccgc ctcacggccg gccgttgcga cgctgtgccg cgcgctcgag
    16441 gcgctgttcg atctgctcct cggtcagccc ccgctcgcgc atacgctgct tgacctgttc
    16501 gatctgctcc ggcgacatcg acgacg
//
LOCUS       DASPGN010000114         6289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_20099, whole genome shotgun sequence.
ACCESSION   DASPGN010000114 DASPGN010000000
VERSION     DASPGN010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20099"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VD788_03180"
     CDS             complement(<1..1322)
                     /locus_tag="VD788_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HXV75295.1"
                     /translation="MAQKPARDRLEGYRRKRSAGRTPEPFSGPSTARPRLFVVQKHAA
                     RNMHFDLRLEMEGVLQSWAVPKGPSLDPQDKRLAVLVEEHPLEYAEFEGIIPAGNYGA
                     GAVIVWDRGRWDPIGDPLAGVRDGKLLFDLHGYKLRGRWTLFRTKRKSTEWLLMKKPD
                     PAAEPRPFDERSIRSGLTVEQLRDGYDPAAALRRRLTRLDPPRRRFALESLSPMLGRA
                     RDRPFSGGGWLFEIKYDGFRLLAERDGATARLRFRGGGDATAALPEIADALRALPFPS
                     FVVDGEVVVLDEQGKPSFQLLQQRIRLSRGGDATRLTVDRPATLFVFDLLALDDRDLR
                     PLPLVERKRLLEKIVPPLGPLRYCEHIVGRGEEMYRAAQELGLEGVMAKRSAAAYVAG
                     RSDDWLKIRADRTGNFAIVGYSPPKRARGGFAALHVAAYDGSRLVYAGR"
     gene            1400..2266
                     /locus_tag="VD788_03185"
     CDS             1400..2266
                     /locus_tag="VD788_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXV75296.1"
                     /translation="MSARAISSAMVSFGLVSIPVKVYSSGEASSGIHFNMLHKKCGSR
                     LKQQYVCAQDGERVERAEMVRGYEFAKGQYVLFSDEELKALQQKADQQIEITEFLPTD
                     KVDPIYFENSYYLGPDKGGDRAYRLLTQALRKSGRSALAKYATRGKQYLVLLRPVEQG
                     LVMQQLRYATEIRSISEIPLGDAQVKPAELDLAMQIIEQAISERFDPASYEDEVRKRM
                     LSAIERKVEGHEITEEPTEEPKAQVIDLMQALKASLSGGTARKPSRRAPPKPAATTKA
                     KKKTTAKKSRTG"
     gene            2314..3147
                     /locus_tag="VD788_03190"
     CDS             2314..3147
                     /locus_tag="VD788_03190"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75297.1"
                     /translation="MQGYSAKDVARMLDLSVAQVRSFVKAGFIEPRRGERGEYRFSFQ
                     DLVLLRTAKGLRAANVPPRRLVAVLRRLKQQLPEGRPLSAVQIQARGDQVVIRRDGTL
                     WDAESGQASFDFDVAELAEKAAPHAARVFERARSAPGALSVEDWFELGFELEAAAPDQ
                     ARQAYLEALERRPSHVDARINLGRLLQLVGDLHLAEAHFRIALSYEPSNATALFNLGV
                     ALEDLGRVGEARRAYGEAIAADETFADAHYNLARLFEQTGDSQAALRHLKIYRRLTES
                     S"
     gene            3149..3997
                     /locus_tag="VD788_03195"
     CDS             3149..3997
                     /locus_tag="VD788_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75298.1"
                     /translation="MRSSARSCRAALVGGLLVGLLCAPAAGGPPPEADPLNHAYAVYL
                     GSGLYVTGDRTVFVMRVAPTVRLRSEEEHPFGIRLRFATTLGFYDLTSNDLAEFDINQ
                     LGTFAFIPGVEFPIRMSDKWTLSPFIDFGPATDTSFHDVTLVVGIGLRSRAEFPTKRH
                     LHLLWNQFIYARNSQTGVRASTDDYTVFRTDYELRGIVSYRLGQRAFDLGLLARSELF
                     FDSVIVDLPLGEPVSTNDRWEIGFTTGSTRKWKHFNMVTSPRIGISYRFGDGASGVRL
                     TVRFRN"
     gene            4134..4748
                     /locus_tag="VD788_03200"
     CDS             4134..4748
                     /locus_tag="VD788_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXV75299.1"
                     /translation="MMKRTQRLLVLFAHPALEKSRVNSRMVDAVRRLPGVQFHDLYEA
                     YPDFAIDVEYEQSLLAASDVVVMQHPLYWYSTPALLKEWEDLVLVHGWAYGTGGTALA
                     GKTMFSVITTGGGEESYCAEGYNRFSLRQLLAPIEQTAILCGMDYLPPFVVHGTHRFG
                     EREIVRHAADYRRLIQALVDGTLDVGAARSFVRLNSDLDAVISS"
     gene            4764..>6289
                     /locus_tag="VD788_03205"
     CDS             4764..>6289
                     /locus_tag="VD788_03205"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HXV75300.1"
                     /translation="MEHGQFLFQAMVYLAAAVIAVPLAKRLGLGSVLGYLVAGVAIGP
                     VGLGMIGAEGQDVMHFAEFGVVMMLFLIGLELQPALLWRLRAPILGLGNLQVAVTTLV
                     IGGFAIGVGLDWREALAIGMTLSLSSTAIVLQTLNEKGLIQSAGGQRSFAVLLFQDIA
                     VIPMLALLPLLGRAAHDPATGGQSWRDGLPGWASTLIVAGAVVGVVLIGRFVVQPAFR
                     AIARTHLREVFTAAALLLVVGIAELMTAVGLSPALGTFLAGVVLANSEFRHELESDIE
                     PFKGLLLGVFFIAVGASIDFRLVAGEPLLIAGLVGALVAVKFSILLLLGRLFRVGTDQ
                     SLLFALALAQGGEFCFVLFSFAEQSRVLGAELAAPLVAVVALSMMTTPLVMWVHERLI
                     RPRIGTRETTEREADRIEERNPVIIAGFGRFGHIVGRLLAANGVGCTVLDVDSDQVDV
                     LRRLGFKVFYGDASRHDLLRAAGAEHARLLVLATDQAEKRLEIVSTVQKHFPQLTILA
                     R"
BASE COUNT          939 a         2110 c         2183 g         1057 t
ORIGIN      
        1 cgcccggcgt agaccaaccg gctgccgtcg taggccgcga cgtgcagcgc agcgaagccg
       61 ccgcgggcgc gtttgggtgg gctgtacccg acgatcgcga agttgccggt gcgatccgcg
      121 cggatcttga gccagtcgtc cgagcgcccg gcgacgtacg ccgccgcgga gcgcttggcc
      181 atcacaccct cgagacccag ctcctgcgcc gcacggtaca tctcctcacc gcgcccgacg
      241 atgtgctcgc agtagcgcag cggtccgagc ggcgggacga tcttctccaa caaccgcttg
      301 cgttcgacga gcggaagcgg gcgcaggtcg cggtcgtcca aggccagcag gtcgaagacg
      361 aacagcgtcg ccgggcgatc gacggtcagg cgggtggcgt cgccgccgcg cgacaggcgg
      421 atccgctgct gcagcagttg gaaggacggc ttgccctgct cgtcgagcac gacgacctcg
      481 ccgtcgacga cgaacgacgg gaacgggagc gcgcggagcg cgtcggcgat ctcgggcagt
      541 gccgcggtcg cgtccccgcc cccgcgaaag cggagccgcg cggtcgctcc gtcgcgctcg
      601 gccagcaggc ggaacccgtc gtacttgatc tcgaacagcc agccgccgcc gctgaacggc
      661 cggtcgcgcg cccgcccgag catcggcgac aacgactcga gcgcgaaccg gcggcggggt
      721 ggatcgagac gggtcaaccg gcggcgcagc gcggccgccg ggtcgtagcc gtcgcgaagc
      781 tgctcgaccg tcagcccgga tcggatcgag cgttcgtcga acggacgcgg ctccgccgcg
      841 ggatccggct tcttcatcag cagccactcg gtcgacttgc gcttggtgcg gaacaacgtc
      901 cagcgcccgc gcaacttgta gccgtggagg tcgaacagca gcttgccgtc acgcacgccc
      961 gcgagcgggt cgccgatcgg gtcccagcgg ccgcggtccc agacgatcac cgcgcccgca
     1021 ccgtagttgc cggccggaat gatcccctcg aactcggcgt actccagcgg gtgctcctcg
     1081 accaacaccg cgagccgctt gtcctgcggg tcgagcgacg ggcccttcgg cacggcccac
     1141 gactgcagca cgccctccat ctcgagacgc aggtcgaagt gcatgttgcg cgcggcgtgc
     1201 ttctgcacga cgaacagccg cggccgcgcg gtgctcggcc cggagaacgg ttcgggcgtg
     1261 cggcccgcgg atcgtttgcg gcggtagccc tcgagccgat ccctggccgg tttctgcgcc
     1321 acgctttcgt ccaatgttcg ccgtcgtctc gccccgggtt gtcgttcgac gacggggagt
     1381 tatgttacgc tctgcggcca tgagcgcacg ggcgatttcg tcggcgatgg tgtcgttcgg
     1441 gttggtatcc atcccggtca aggtctattc ctccggcgag gcgtcgtccg ggatccactt
     1501 caacatgctg cacaagaagt gcgggtcgcg cctgaagcag caatacgtct gtgcgcagga
     1561 cggtgagcgt gtcgagcggg ccgagatggt ccggggctac gagttcgcga aggggcagta
     1621 cgtgctgttc agcgacgagg agctcaaggc gctgcagcag aaggccgacc agcagatcga
     1681 gatcaccgag ttcctgccga ccgacaaggt cgacccgatc tacttcgaga acagctacta
     1741 cctcgggccg gacaagggcg gcgaccgcgc ctaccgattg ttgacgcagg cgctgcgcaa
     1801 gagcggccgc tcggcgctgg ccaagtacgc cacacgcggc aagcagtatc tggtgctgct
     1861 gcgaccggtc gagcagggtc tcgtgatgca gcagctgcgt tacgcgaccg agatccgctc
     1921 gatcagcgag atcccgctgg gcgacgcgca ggtgaagccc gccgagctcg acctcgcgat
     1981 gcagatcatc gagcaagcga tctccgaacg tttcgacccg gcgagctacg aggacgaggt
     2041 gcgcaagcgc atgctgtccg cgatcgagcg gaaggtcgaa ggccacgaga tcaccgagga
     2101 gccgacggaa gaaccgaagg cccaggtgat cgacctgatg caggcgctca aggccagtct
     2161 gtccggcggg acggcgcgca agccatcccg acgtgccccg cccaagcccg ccgcgacgac
     2221 caaggcgaag aagaaaacga cggcgaagaa gagccgcacg ggctgagccg ctgtttcgcg
     2281 cgggtcggcg ttcgtgcata atccgcacga acgatgcagg ggtacagcgc gaaagacgtg
     2341 gcccgcatgc tcgacctgtc ggtcgcgcag gtccgatcgt tcgtcaaggc cggcttcatc
     2401 gaaccgcgtc gcggcgagcg cggcgagtac cggttctcgt tccaggatct cgtgctgttg
     2461 cgcacggcca agggtctgcg cgcggcgaac gtgcccccgc gccgcctggt tgcggtgctg
     2521 cgacggctga aacagcagct gcccgagggc cggccgttga gcgccgtgca gatccaggcg
     2581 cgcggcgacc aagtcgtgat ccgtcgcgac gggaccctgt gggacgcgga gtcgggccag
     2641 gccagcttcg atttcgatgt cgccgagctg gccgagaagg ccgcacccca cgccgcccgc
     2701 gtcttcgaac gcgcgcggag tgcccccggc gcactgtcgg tcgaggactg gttcgagctg
     2761 ggcttcgagc tcgaggccgc ggcgccggat caggcccggc aagcgtacct cgaggcgctc
     2821 gagcgccggc cgtcccacgt cgacgcgcgg atcaacctcg gccggctgtt gcagctggtg
     2881 ggtgatctgc acctggccga ggcgcatttc cgcatcgcgc tgagctacga gccgtcgaac
     2941 gccacggcgt tgttcaatct gggcgtcgcg ctcgaggacc tcggacgggt cggtgaagca
     3001 cgacgcgcgt acggcgaggc gatcgcggcc gacgagacct tcgccgacgc gcactacaac
     3061 ctggcccgcc tgttcgagca gaccggcgac agccaggccg cgttgcgcca cctgaagatc
     3121 taccgcaggc tgaccgaatc gagttgacgt gagatcgtcc gcgcgttcgt gccgcgccgc
     3181 gctggtcggc gggttgctcg tcgggctgct gtgcgcgccg gcagccggcg ggcccccgcc
     3241 ggaggcggac ccgttgaatc acgcctacgc ggtctatctc ggctcgggtc tctacgtgac
     3301 gggcgaccgc acggttttcg tgatgcgcgt cgcaccgacg gtccggctgc gctccgagga
     3361 ggaacacccg ttcggcatcc gcctgcgctt cgcgacgacg ctcggtttct acgacctgac
     3421 ctcgaacgac ctcgccgagt tcgacatcaa ccaactgggc acgttcgcct tcatcccggg
     3481 agtcgagttc ccgatccgaa tgagcgacaa gtggactctg tcgccgttca tcgacttcgg
     3541 cccggcgacc gacacgtcgt tccacgacgt cacgctcgtc gtcggcatcg gcctccgcag
     3601 ccgggccgag ttccccacca agcgccatct gcacctgttg tggaatcagt tcatctacgc
     3661 ccgcaactcg cagaccggcg ttcgtgccag caccgacgac tacaccgtgt tccgcaccga
     3721 ctacgagctg cgcgggatcg tctcctaccg tctgggccag cgcgcgttcg acctcggtct
     3781 gctcgcccgg tccgagctgt tcttcgactc ggtgatcgtc gatctgccgc tgggcgagcc
     3841 ggtgtcgacc aacgatcgct gggagatcgg cttcaccacc ggttcgacgc gcaagtggaa
     3901 gcacttcaac atggtcacct cgccgcgtat cgggatcagc taccgcttcg gcgacggcgc
     3961 gtcgggtgtg cgtctcaccg ttcgctttcg caactgacgg ccgagccctg gcgaacggat
     4021 ggtcggccgc gtccgtccgt ggttcaatgt agcccggcat cgccgcccgc acggaggttt
     4081 cgcaagcacg agccggcagt cctcgacctt tcgatgtccg gtgacccgac cgcatgatga
     4141 agcggaccca gcgtctactg gtcctgttcg cccacccggc gctggagaag tcccgggtca
     4201 attccaggat ggtggacgcg gtgcggcggc tgccgggcgt acagttccac gatctgtacg
     4261 aggcctatcc cgacttcgcc atcgacgtcg agtacgagca gtcgctgctc gccgcgagcg
     4321 acgtggtcgt gatgcagcac ccgctctatt ggtacagcac ccccgccttg ctcaaggagt
     4381 gggaagacct ggtgctggtg cacggctggg cttacggcac gggcggcacc gcgctcgccg
     4441 gcaagacgat gttcagcgtc atcacgacgg gcggcggcga ggagtcctat tgcgcggaag
     4501 gctacaaccg gttctccttg cggcagctgc tcgccccgat cgagcagacg gcgatcctgt
     4561 gcgggatgga ctatctgccg ccgttcgtcg tccacgggac ccatcggttc ggcgagcgcg
     4621 agatcgtgcg gcacgccgcc gactaccggc gattgatcca ggcgctcgtc gacggcacgc
     4681 tcgacgtcgg cgcggcccgc tcgttcgtcc ggctgaactc cgatctggac gcggtgatct
     4741 cgagctgagc ggaggcgatc gaggtggagc acggacagtt cttgttccag gcgatggtct
     4801 atctcgcggc cgcggtgatc gccgtgccgc tggccaagcg gctcgggctc ggttcggtgc
     4861 tgggctatct cgttgccggg gtcgcgatcg gaccggtcgg gctgggcatg atcggtgcgg
     4921 agggtcagga cgtgatgcac ttcgccgagt tcggcgtggt gatgatgctg ttcctgatcg
     4981 gactcgagct gcagccggcg ctgctgtggc ggttgcgcgc accgattctc ggcctgggca
     5041 acttgcaggt cgccgtcacc acgctcgtga tcggcggctt cgcgattggc gtcggcctcg
     5101 actggcgcga ggcgctcgcg atcggcatga cgctgtcgct gtcgtcgacg gcgatcgtgc
     5161 tgcagacgct caacgagaag ggcttgatcc aatcggccgg cggacagcgc tcgttcgccg
     5221 tgctgctgtt ccaggatatc gcggtgatcc cgatgctggc gctgctgccg ttgctgggcc
     5281 gggcggcgca cgaccccgcg acaggcggcc agagctggcg tgacggcctg ccgggctggg
     5341 ccagcacact gatcgtcgcc ggcgcggtgg tcggcgtggt actgatcggt cggttcgtcg
     5401 tccaacccgc gttccgcgcg atcgcgcgca cgcacctgcg cgaggtgttc accgcggccg
     5461 cgctgctgct cgtcgtcggc attgccgagt tgatgaccgc tgtcgggctg agccctgcgc
     5521 tgggtacctt tctcgccgga gtggtgctgg ccaacagcga gttccgccac gagctcgagt
     5581 ccgacatcga accgttcaag gggctgctgc tcggcgtctt cttcatcgcc gtcggtgcat
     5641 cgatcgactt ccgcctggtc gccggtgagc cgctgctgat cgcgggtctg gtcggcgcac
     5701 tggtcgcggt gaagttctcg atcctgctgc tgctcggccg tctgtttcgc gtcgggaccg
     5761 accagagttt gttgttcgcc ttggcgctgg cccagggtgg cgagttctgc ttcgtgctgt
     5821 tctcgttcgc cgagcagagc cgggtgctgg gcgccgagct ggccgcgccg ctcgtcgcgg
     5881 tcgtggcgct gtcgatgatg acgacgccgc tcgtgatgtg ggttcacgag cggctgatcc
     5941 ggccgcggat cggcacgcgc gagacgaccg agcgcgaggc cgatcggatc gaggagcgca
     6001 acccggtgat catcgcgggc ttcgggcgct tcggtcatat cgtcggccgt ttgctcgcgg
     6061 ccaacggcgt cggttgcacg gtgctcgacg tcgattcgga tcaggtcgac gtgctgcgcc
     6121 gactcggttt caaggtgttc tacggcgacg cctcgcgtca tgacctgctg cgggccgcgg
     6181 gagccgagca cgccaggctg ctggtgctcg cgaccgatca ggcggaaaag cgcctcgaga
     6241 tcgtctcgac ggtgcagaag cacttcccgc agctgacgat cctggcccg
//
LOCUS       DASPGN010000115         7806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_840019, whole genome shotgun sequence.
ACCESSION   DASPGN010000115 DASPGN010000000
VERSION     DASPGN010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840019"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1224
                     /locus_tag="VD788_03210"
     CDS             <1..1224
                     /locus_tag="VD788_03210"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HXV75301.1"
                     /translation="ETDALLAHELAHLLRRDHWVRYVELSATALFWWHPVAWWARARL
                     RRAEESCCDQIVLRTFADPRAYARALIETVEYLVDTGRPVPALASGVGEARQMEERLT
                     MIMNERPPSRPSRTVRLVLAVVALALLLVFPTWSDRAAAAGEGRAPVGSPIDENESEL
                     RESMLELERLALDLERQLREIRGRQNELNLELERHRVARGIDELESGAESIDDGSREA
                     RERLLFERLDQIERQTSLTARALELERAELEQTAELELALRRRMLDVERSQGAGDRRA
                     AERMAAAALELERRIESSARELDERRLQLQQEELDAQAEAMRAEIDLLREQGRRIEAD
                     ELERELDRRWVIESERRGAGRVEERARVEAALAELTELARRLEREGRERELREVEKQI
                     ETLRRQIEAGSGEPY"
     gene            1326..1721
                     /locus_tag="VD788_03215"
     CDS             1326..1721
                     /locus_tag="VD788_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75302.1"
                     /translation="MTQGELVRIEQAVGVSLPADLRELYTHYPFAPDSWAASCAMPDD
                     CPLLIRWNSCEPAAPSFGLDVARAFQIGTDGRDRLYFARLGGRETAILEADLDSGRLS
                     THDPDLLSWVLFLHAMHDRVDRDQQPTRP"
     gene            1808..3283
                     /locus_tag="VD788_03220"
     CDS             1808..3283
                     /locus_tag="VD788_03220"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HXV75303.1"
                     /translation="MGARVGSYGVSLSLSLAIACAPGGRPEDGAEVEALAEQAYLYGI
                     QQVRFYGARFDYTQNETSDVYAGINRFSSLNDGRSIPAEPRAIVRTDVHTIEARAFLD
                     LRHQPLVLETPEVADRYFSLQLMDAYGVYFFHAGSQWNGAEARRYLLLPAGWQDPPPS
                     GFPAINVIHAPANSVLAVLRLARKDPSDPAEVAEVNRLRSLVTITPLGEWSPLPRRPL
                     DPDLPPIVPGDYPSAPRPEQLTPRPLERQTAEEFFNLLHLVLNDTSLARIADSTQESE
                     MLNRLAAIGVGEGLDFDWAALDARTRRALESGFARGFSVVRRAGTRATVDFGGWKMLR
                     ASGRFRTDWLARAVTADFDWAGPDRDGSHATAVLDQDADGRRLDGRQRYTLRLSMGRL
                     PPVTEFWSIAIYDDAGRLVDNELGRHAVTSFMLDARELAVHNGELRIQIQHARPSDSA
                     EAANWLPAPTGPFRLVARYFGPYTAVFNGAYRLPPVQRRLD"
     gene            3413..>7806
                     /locus_tag="VD788_03225"
     CDS             3413..>7806
                     /locus_tag="VD788_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HXV75304.1"
                     /translation="MRRLVLIIVVVLAAWLSLTAPPTHAQTTVYVDDDNCAGGGGDGS
                     QIDPFCTIQQGICSIEDTGGGTVLVGPGTYNESLRMFPGVSVIATDGPAVTTIDATGV
                     PCTTRYCVPSEINLVCSAVVFGEGSTTDDRLEGFRITGGSGLFREFSGSSTPNAITGG
                     GIFVFDSSPTITNNEIVDNELSNAVTGQFWGGGIYLFGTIPASPITPRITHNLIEGNL
                     AVSPKGKNNQPTFSVGGGIYSSWYVAARIEGNSIRQNVAGIASVNDQIGGGGGITIYQ
                     QMPGQIPIITGNLFQQNTSADFGGGFAFGQTSDGVNIFPTHGTVENNVFEQNQSFSGG
                     GGSVGTNRAVVRNNTFVDNTASFGGGFVAGYAPNPGDQVTFVNNLVIFNTATPYDAGG
                     LGAYDALPLFAYNDIYGNSPDEVAGQYLESDIIGQFGNVSVDPLVVDPAPGSRDLHLT
                     ATSPILDIGDNNQAPAADYDGNPRIQDGDGVGGAVVDPGAFEFASPVIDSDGDGLFDD
                     QDNCPAVPNPSQTDTDGDGDGDACDACPLDAMNDADGDGVCADADNCPHTINPGQTDT
                     DGDGLGDACDPCPLNSDVDTDGVCDALDNCPDVANPDQTDGDLDEIGDDCDACPLDPV
                     NDVDGDGVCADADNCPTIANAGQADGDADGAGDPCDPCPADADDDADTDGLCADADNC
                     PAIANVDQTDGDADGAGDPCDTCPIDAANDVDADGLCADVDNCPAIANVDQTDGDADG
                     AGDPCDPCPADPVDDADDDGFCADADNCPAEPNPLQQDGDGDGIGDACDPCALDPAND
                     ADGDGFCADLDNCPAVANPLQQDGDADGVGDACDACLLDPANDADGDGHCADLDNCPT
                     DANPLQQDADQDGVGDACDSCALDPDNDLDADGVCGDVDNCPAVANPLQTDGDGDGAG
                     DACDLCPADADDDTDDDGVCGDVDNCPAVANPNQSDGDGDGAGDACDLCPADADDDAD
                     ADGVCGDADNCPAAANPAQTDLDGDGAGDACDLCPADADDDADDDGVCGDVDNCPAVV
                     NAAQTDDDTDGLGNACDACANDAANDADGDGVCGDVDNCPLTSNAGQSDNDGDGLGNA
                     CDACANDAANDADADGVCGNLDNCPSVANAGQADGDGDGIGDVCDACPDDGANDADAD
                     GFCADLDNCPSVTNADQADGDGDGIGDLCDVCPGSAQDDSDGDGVCDDGDNCPATANA
                     SQIDRDGDGAGDACDACPLDAANDVDTDGVCGNVDNCPTVANANQADADTDADGDACD
                     NCPLDAANDVDADGLCADADNCPTEANPNQTDADADGAGDACDSCPLDGMDDADGDGL
                     CADVDNCPTVANANQADTDADGIGNPCDACPLDAANDVDQDGVCGDADNCPTVPNAAQ
                     TDADADGAGDVCDACPLDAADDVDQDGVCGDLDNCPAAPNAAQTDADADGAGDACDAC
                     PLDLAFDADLDGACDEVDNCPLVANATQV"
BASE COUNT         1308 a         2776 c         2681 g         1041 t
ORIGIN      
        1 gagaccgacg cactgctggc gcatgaactg gcccacctgc tacggcggga ccactgggtg
       61 cgctacgtcg agctgtcggc gacggcgctg ttctggtggc atccggtcgc gtggtgggcg
      121 cgggcgcggc tgcggcgcgc cgaagagtcc tgctgcgacc agatcgtgct gcggaccttc
      181 gccgatccgc gggcctacgc ccgggcgttg atcgagaccg tcgaatacct cgtcgacacg
      241 ggacgacccg ttccagcgct ggccagcggt gtcggcgagg cccgtcaaat ggaagagagg
      301 ttgacgatga tcatgaacga acgccctccg tcccgcccgt cgcgtaccgt gcgcctggta
      361 ctcgccgtcg tcgcgctggc gctgctgctc gtttttccca cgtggtccga ccgcgcggcg
      421 gcagccggcg aaggacgcgc ccccgtgggg tcgccgatcg atgagaacga gagcgagttg
      481 cgcgagagca tgctggagct cgaacggctc gccctcgatc tcgagcggca gctgcgcgag
      541 atccgcggcc ggcagaacga gctcaacctc gaactggagc gacaccgggt cgcgcgggga
      601 atcgacgagc tcgaatccgg ggcggagtcg atcgacgacg gcagccggga ggcgcgggaa
      661 cgcctgcttt tcgagcggtt ggaccagatc gaacgccaga cctcgctcac cgctcgcgcg
      721 ctggagctcg aacgggccga gctcgagcaa acggccgagc tcgagctggc gctgcgccgg
      781 cggatgctcg acgtcgagcg gtcgcagggt gccggcgacc ggcgggcggc ggaacggatg
      841 gccgccgcgg cgctggagct cgagcgacgg atcgaatcga gcgcgcggga gctggacgag
      901 cgccggctgc agctgcagca ggaggagctc gacgcgcagg ccgaggcgat gcgggccgag
      961 atcgacctgc tgcgcgaaca gggacggcgg atcgaggccg acgagctcga acgagagctc
     1021 gaccgccggt gggtgatcga atcggagcgg cgcggcgccg gtcgggtcga ggagcgcgcg
     1081 agagtcgagg cggcgctggc cgagctcacc gagctggcac gtcgtctcga gcgcgaggga
     1141 cgcgagcggg agctgcgcga ggtcgagaag cagatcgaga cgctccgacg ccagatcgag
     1201 gccggatcgg gcgaaccgta ctgagacgcg ggccgcggct gcgcgtgggc tgtgaggaac
     1261 ccgtgtcgtc ggagagccgg ccgagccggt tgcgaacttc cgagacagga cactagattc
     1321 gccgcatgac ccagggcgag ctggtccgca tcgagcaggc ggtcggtgtc tcgctgccgg
     1381 ccgacttgcg cgagctgtac acgcactacc cgttcgcgcc cgactcgtgg gccgcgagct
     1441 gcgcgatgcc cgacgactgt ccgctgttga tccgctggaa cagctgcgag ccggcggcac
     1501 cgagcttcgg cctcgacgtc gcgcgggcgt tccagatcgg caccgacggg cgggaccggc
     1561 tctacttcgc caggctcggc gggcgtgaga ccgcgatcct cgaggccgat ctcgacagcg
     1621 gccggctctc gacgcacgac cccgatctgt tgtcttgggt gctgttcctc cacgcgatgc
     1681 acgaccgcgt cgaccgcgac cagcagccga cccggccttg acgccggtcg acccgtcgtc
     1741 gagccggcga cgacgaccgc tcatccgcta gaatcggtcc gattcttcgt acgggggtcg
     1801 ggcagggatg ggcgcgcggg tcggttccta cggggtctcg ttgtcgttgt cgttggcgat
     1861 cgcctgcgct cccggcggcc ggccggagga cggcgccgag gtcgaggcgc tggccgagca
     1921 ggcctatctg tacgggatac agcaagtgcg gttctacggc gcgcgtttcg actacaccca
     1981 gaacgaaacg agcgacgtct acgccggcat caaccgcttc agctcgttga acgacggccg
     2041 gtcgattccg gccgagccac gggcgattgt caggaccgat gtccacacga tcgaggccag
     2101 ggcgtttctc gacttgcgcc accagccgct cgtgctcgag acgcccgagg tcgccgatcg
     2161 ctatttcagc ctgcagttga tggacgccta cggcgtctac ttctttcacg cgggcagcca
     2221 atggaacggt gccgaggcgc ggcgctacct gctgttgccc gccggttggc aggaccctcc
     2281 accgagcggc ttcccggcca tcaacgtgat tcacgcgccg gcgaactcgg tgctcgcggt
     2341 cctccgcctc gcgcggaagg acccgtcgga tccggccgag gtcgccgagg taaaccggct
     2401 ccggtcgctc gtgacgatca cgccgctcgg cgagtggtca ccgctccctc gccggccgct
     2461 cgatcccgac ctcccgccga tcgtgccggg cgactacccc agcgctccgc ggccggagca
     2521 gctgacaccg cgcccgctcg agcggcagac ggcggaggag ttcttcaacc tgctgcatct
     2581 cgtgttgaac gacacgtcgc tcgcgcgaat cgccgattcg acgcaggaaa gcgagatgct
     2641 gaaccgactc gccgcgatcg gtgtgggcga gggtctcgat ttcgattggg cggcgctcga
     2701 cgcgcgaacc cgccgcgcgc tcgaaagcgg cttcgcgcgg ggcttctccg tcgtgcgacg
     2761 cgcgggcacg cgcgcgacgg tcgacttcgg cggttggaag atgctgcggg cgtccggccg
     2821 gttccgcacc gattggctgg cgcgggcggt gacggccgat ttcgactggg ccggaccgga
     2881 tcgcgacggc tcgcatgcca ccgcggtcct cgaccaggat gccgatggga gaaggctcga
     2941 cggccgccag cgctacacgc tgcggctgtc gatgggccga ctgccaccgg tgaccgagtt
     3001 ctggtcgatc gcgatctatg acgacgccgg gcggctcgtc gacaacgagc tcggtcgcca
     3061 tgcggtcacc agcttcatgc tcgatgcgcg cgagctcgcc gtgcacaacg gcgagctgag
     3121 gatccagatc cagcatgcac gaccgagcga ttccgccgag gccgcgaact ggcttcccgc
     3181 cccgacgggg ccgtttcgtc tcgttgcgcg gtacttcggt ccttacaccg cggtcttcaa
     3241 cggagcctat cgcctacccc cggtccagcg ccggctcgac tgagccactc atccgaggct
     3301 ccggttgtcc gttgccccgg ccggaacggg cgcgtatatg agaggggcac ggtcgcgggc
     3361 gcgcccttcg acggaaccgc ggttttcgtc ggggggacaa ctgagaagcg gattgcgccg
     3421 attagttcta attattgtcg tagtcctggc ggcctggctc tcgctcaccg cacccccgac
     3481 ccacgcccag accacggtct acgtcgacga cgacaactgc gccggcggtg gcggggacgg
     3541 cagccagatc gatccgttct gcacgatcca gcaagggatc tgctcgatcg aggatacggg
     3601 cggcgggacg gtgctcgtcg ggccgggtac ctacaacgag tcgctgcgca tgttccccgg
     3661 cgtcagcgtg atcgcgaccg acggaccggc ggtgacgacg atcgacgcga ccggtgtccc
     3721 gtgcacgaca cgctactgcg tgccgagcga gatcaacctc gtctgttcgg cggtcgtctt
     3781 cggcgagggc tcgacgaccg acgacaggct cgaaggcttt cgcatcaccg gcggctcggg
     3841 gctgttccgc gagttttccg ggtcgagcac gccgaacgcg atcaccggcg gagggatctt
     3901 cgttttcgac agctcgccga cgatcaccaa caacgagatc gtcgacaacg agctcagcaa
     3961 cgccgtcacc ggtcagttct ggggcggcgg gatctacctg ttcggaacga tcccggcctc
     4021 accgatcacc ccgcggatca cccacaacct gatcgaaggc aacctcgccg tttcgccgaa
     4081 gggaaagaac aaccagccga ccttctcggt cggcggcggg atctactcga gctggtacgt
     4141 ggccgcgcgg atcgagggca actcgatccg gcagaacgtc gccggcatcg cctccgtcaa
     4201 cgaccagatc ggcggcggtg gtggcatcac gatctaccaa cagatgcccg gtcagatccc
     4261 gatcatcacc ggcaacctgt tccagcaaaa cacgagcgcc gacttcggcg gcgggttcgc
     4321 cttcgggcag acctcggacg gggtgaacat cttcccgacc cacggcaccg tcgagaacaa
     4381 cgtcttcgag cagaaccaga gcttctcggg cggcggaggc agcgtcggca cgaaccgcgc
     4441 cgtcgtccgc aacaacacgt tcgtcgacaa caccgcgtcg ttcggcggcg gcttcgtcgc
     4501 gggctacgca ccgaacccgg gggaccaggt caccttcgtc aacaacctgg tgatcttcaa
     4561 tacggcgacg ccgtacgacg ccggcggtct gggtgcctac gacgcgctgc cgctgttcgc
     4621 ctacaacgac atctacggca actcgccgga cgaggtggcg ggtcagtacc tcgagtcgga
     4681 catcatcggc cagttcggca acgtctcggt cgatccgctg gtcgtcgacc cggcgccggg
     4741 ctctcgcgac ctgcacctga ccgcgacgag cccgatcctc gacatcggcg acaacaacca
     4801 ggccccggca gcggactatg acggcaaccc gcgcatccag gacggcgacg gcgtcggcgg
     4861 ggcggtcgtc gacccgggcg cgttcgagtt cgcgagcccg gtgatcgaca gcgacggtga
     4921 cggcctgttc gacgatcagg acaactgtcc ggcggtcccc aacccgagtc agaccgacac
     4981 ggacggcgat ggcgacggcg acgcctgcga cgcctgcccg ctcgacgcga tgaacgacgc
     5041 cgacggcgac ggtgtttgtg ccgacgccga caactgcccg cacacgatca accccgggca
     5101 gaccgatacg gacggcgacg gtctcggcga tgcttgcgat ccgtgtccgc tcaacagcga
     5161 cgtcgacacg gacggggttt gcgacgcgct cgacaactgt cctgacgtcg ccaatcccga
     5221 ccagaccgac ggcgatctcg acgagatcgg cgacgactgc gacgcctgtc cgctcgaccc
     5281 ggtcaacgac gtcgacggcg acggtgtttg tgccgacgcc gacaactgcc cgacgatcgc
     5341 caacgccggt caggccgatg gcgacgccga cggcgcgggc gatccttgcg acccgtgccc
     5401 cgcggatgcc gacgacgacg ccgacaccga cgggctctgc gccgacgccg acaactgccc
     5461 ggcgatcgcc aacgtcgatc aaaccgacgg cgacgccgac ggcgcgggcg acccctgcga
     5521 cacctgtccg atcgacgcgg ccaacgacgt cgacgccgac ggcctctgcg ccgacgtcga
     5581 caactgcccg gcgatcgcca acgtcgatca gaccgacggc gacgccgacg gcgcgggcga
     5641 cccctgcgac ccgtgccctg cggatccggt cgacgacgcc gacgacgacg ggttctgtgc
     5701 ggacgccgac aactgcccgg ccgagcccaa cccgctccaa caggacggcg acggtgacgg
     5761 catcggcgac gcgtgcgacc cctgcgcgct cgacccggcc aacgacgccg acggcgacgg
     5821 gttctgcgcc gatctcgaca actgtccggc cgtcgccaac ccgcttcagc aagacggcga
     5881 cgccgacggc gtcggcgacg cgtgcgacgc ctgcctgctc gacccggcca acgacgccga
     5941 cggcgacggc cactgcgccg atctcgacaa ctgtccgacc gacgccaacc cgcttcaaca
     6001 agacgcggac caggacggcg tcggcgatgc ctgcgacagc tgcgccctcg atccggacaa
     6061 cgacctcgac gccgacggcg tctgcggtga cgtcgacaac tgtccggccg tcgccaaccc
     6121 gctccagacc gacggcgacg gcgacggggc tggcgacgcc tgcgacctct gcccggccga
     6181 tgccgacgac gacaccgacg acgacggcgt ctgcggcgac gtcgacaact gtccggccgt
     6241 ggccaatccg aaccaatccg acggcgacgg cgacggggcc ggcgacgcct gcgatctctg
     6301 tccggccgac gctgacgacg acgccgacgc ggatggcgtg tgcggcgacg ccgacaactg
     6361 ccccgccgcg gccaaccccg cccaaaccga tctcgacggc gacggcgcgg gcgacgcctg
     6421 cgacctctgc ccggccgatg ccgacgacga cgccgacgac gacggcgtct gcggcgacgt
     6481 cgacaactgc ccggccgtgg tcaacgccgc ccagaccgac gacgatacgg acggtctcgg
     6541 aaacgcttgc gatgcctgcg cgaacgacgc ggccaacgac gccgacggcg acggcgtctg
     6601 cggcgacgtc gacaactgcc cgctcacgag caatgccggc cagagcgaca atgacggcga
     6661 cgggctgggc aacgcctgcg atgcgtgtgc caacgacgcg gccaacgacg ccgacgccga
     6721 cggcgtctgc ggcaacctcg acaactgccc gagcgtggcc aacgccggtc aggccgacgg
     6781 tgacggcgac ggcatcggcg acgtgtgcga tgcctgcccc gacgacggcg ccaacgacgc
     6841 cgacgccgac gggttctgcg ccgacctcga caactgcccg agcgtgacca acgccgatca
     6901 ggccgacggt gacggcgatg gcatcggtga cctgtgcgac gtttgtccgg gctcggcgca
     6961 ggacgattcg gacggcgacg gcgtctgcga cgacggcgac aactgcccgg cgacagccaa
     7021 cgcgagccag atcgaccggg acggcgacgg cgcgggcgac gcctgcgatg cctgcccgct
     7081 cgacgccgcg aacgacgtcg ataccgacgg cgtgtgtggc aacgtcgaca actgcccgac
     7141 cgtggccaac gccaaccagg ccgacgccga caccgacgcc gacggcgacg cttgcgacaa
     7201 ctgcccgctc gacgcggcga acgacgtcga cgccgacggt ctgtgtgccg acgccgacaa
     7261 ctgcccgacc gaggcaaacc cgaaccagac ggacgccgac gccgacggcg ccggtgacgc
     7321 ctgcgacagc tgtccgctcg acgggatgga cgacgcggac ggcgacggtc tctgcgccga
     7381 cgtcgacaac tgcccgacgg tggccaacgc caaccaggcc gacaccgacg ccgacggcat
     7441 cggcaacccg tgcgacgcct gcccgctcga cgccgccaac gacgtggacc aggacggggt
     7501 ctgcggcgac gccgacaact gcccgacggt cccgaacgcg gcgcagaccg acgcggacgc
     7561 cgacggcgcg ggcgacgtct gcgacgcctg cccgctcgac gccgctgacg acgtggacca
     7621 ggacggagtc tgtggcgatc tcgacaactg ccccgccgcg ccgaacgcgg cgcagaccga
     7681 tgcggacgcc gacggcgcgg gcgatgcctg cgacgcctgc ccgctcgacc tcgcgttcga
     7741 cgccgatctc gacggcgcct gcgacgaggt cgacaactgc ccgctcgtgg ccaacgcgac
     7801 gcaggt
//
LOCUS       DASPGN010000116         5730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_723326, whole genome shotgun sequence.
ACCESSION   DASPGN010000116 DASPGN010000000
VERSION     DASPGN010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723326"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VD788_03230"
     CDS             <1..218
                     /locus_tag="VD788_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75305.1"
                     /translation="VAGRLGTARRAEEAWVLERIDRGGGPRSLDCGLAPLYSDDDAQI
                     VEPDPETRARYLPGHSATTAGSIDFLP"
     gene            complement(164..1825)
                     /locus_tag="VD788_03235"
     CDS             complement(164..1825)
                     /locus_tag="VD788_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HXV75306.1"
                     /translation="MAITNEAAFTTALERMIDEHRRKCVDLGRQELIRHALENKEAFA
                     SRCGALATWTPSDSTGRSPKDTVIVRNPQSEKKIDWDSPNCIPLDPETFEMLWSDALE
                     VLAGKPRLYVTNRYLGADPAYALPVKTITDRALHSVFVDNMFRARPDGAENSIFADYG
                     FQIVALPYDKIETSRYAGRLRKTGDGTTSDMVIAMDFDRRLGLVYGSAYCGSIKKLMF
                     TVMNFLLPEHGILPLHCSANEGRRGDTALLLGLSGTGKTSLSADPDRALLGDDEHGWG
                     DRGVANFEGGCYAKLINLREEKEAEIYRAVFHEVDPLQHGAIVENCLMYPDGEFDVDD
                     ERLTPNSRGSYPLSFLDNIKTPPVGDHPKTILFLAADANGVLPPVARLNPNQAMLWFL
                     MGYTSKLAGTERGVVDPVTTFSRFFGAPFMPRLPDHYAKMLGDKLAEHGTKVFLVNTG
                     WSGGSFGEGARMDIRLTRRLVEAALNGDLDRVPYREDELFHVGIPESCPGVPSELLFP
                     RNTWKDPRAYDARARKLAAEFSAAFDKNYGKKSIDPAVVAECPGK"
     gene            2178..3404
                     /locus_tag="VD788_03240"
     CDS             2178..3404
                     /locus_tag="VD788_03240"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HXV75307.1"
                     /translation="MRIATAGVLLAATAAWAEGLYDMPIGDLGRAQRTVELVLDAVTD
                     CHTGEVIPPGELPRRLDGVRLLFVGERHISAEFHAVELRVLQELVRAGRTVAIGLEMY
                     PYTEQRYLDQWSAGLLTEQGFVELSDWYDNWGYDWQYYREIFLFARDHGLPMHALNTP
                     REVISAVRQKGFDDLTEEEARHVPPAIDTDSDDHLRLFRAMLEEDSDEDFHAELPDEI
                     LRPMFDAQCTWDATMAHHAVKALAGGQADEILVVLVGTGHLAYELGLQRQARQWFDGR
                     MATLIPISVGDECEPVHRVRASFADFVWGVPEEPSPARPFLGLSTTADPESGRRKVIY
                     VSTDSPAEAAGFAVGDLVLTLDGVELADQASLKRLTSAKQWGDTARFTVARGGETIGL
                     DLRFRRNRPEPCEGDE"
     gene            3404..>5730
                     /locus_tag="VD788_03245"
     CDS             3404..>5730
                     /locus_tag="VD788_03245"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXV75308.1"
                     /translation="MRVVAAGVVTAVLAGPGLGAAAAPVEHEIELALEPQSGRLTVRD
                     RVTVRVGATTGSVVEFVLNERLELDPAAPVEPVGEPFSAARFELNGHQIARGGEATIR
                     RYRLTDPLDRGPFTIAYTGAMAFELGEVHEEYTRGFRETIGQLGPDGVYLTADSFWYP
                     YFGDGLVEYRMTVEQPDGWHVISAGRGRSRDDDDRAQWESEGAVDEIYLAGGPLYLHR
                     DSVGAVETLVYLHEDDPALSERYLTATAQYLEMYRSMIGPYAYSKFALVENFWETGYG
                     MPSFTLLGPRVIRLPFILHSSYPHEILHNWWGNGVFVDYDSGNWCEGLTAYLADHLVQ
                     EQRGQGAAYRRDTLQKYRDYVGENRDFPLEQFRTRHSAATEAVGYGKSLVGFHMLRMA
                     MGDDVFRRALAELYREYRGRRASFDDLRRVFESASGLPLADFFRQWLTRTGAPALAAR
                     VAWCDAGTTLRVCGTLEQTQSGPPFAVDVPIVLDTADGPQEHTVRMKTREQGFALAAT
                     TPVWRVRVDPSFDVLRLLDPRETPPTIGQLFGEPRILAVLPSSDAPRREAYRALVEGW
                     RSASHEIEVVVDAELAALPAGLSVWVLGRQNRFAGPLFGGGVPRGFELLPTSTAFGQQ
                     SVSSAGHSIVSVRRHPASERHAVGLLDVDPEAAFPGIGRKLPHYGKYSYLAFAGDEPE
                     NVIKGQWPTDDSPLVVELAAPPDPVGADPGAAPAERSALAELPPLFSRQALGDHVDYL
                     ASRELEGRGVGTTGLDRAAEYVAARFAAVGLEPAG"
BASE COUNT          846 a         1948 c         1991 g          945 t
ORIGIN      
        1 gggtcgccgg ccgcctgggg accgcgcgcc gcgccgagga ggcctgggtc ctcgagcgga
       61 tcgaccgcgg gggcgggccg cggtcgctcg actgcggcct ggctccgctc tactcggacg
      121 acgacgcgca gatcgtcgag cccgaccctg agacgcgggc gcgctacttg cccggacact
      181 cggcgacgac ggctggatcg atcgacttct tgccgtagtt cttgtcgaac gccgccgaga
      241 actcggccgc gagcttcctg gcgcgcgcat cgtaggcccg cgggtctttc caggtgttgc
      301 gcggaaagag caattcggac ggcacccccg gacagctctc cgggatcccg acgtggaaca
      361 gctcgtcctc acggtacggc acccggtcga ggtcgccgtt caacgcggcc tcgaccagcc
      421 tgcgcgtcag ccggatgtcc atgcgcgcgc cctcgccgaa cgatccgccg ctccagccgg
      481 tgttgacgag gaacaccttg gtgccgtgct cggccagctt gtcgccgagc atcttggcgt
      541 agtggtccgg caggcgcggc atgaacggcg cgccgaaaaa ccgcgaaaag gtcgtcaccg
      601 ggtcgaccac gccccgctcc gtcccggcca gcttgctggt gtagcccatc aggaaccaca
      661 gcatcgcctg gttcggattg agcctggcga ccggaggaag caccccgttg gcgtcggccg
      721 cgagaaacag gatcgtcttg ggatgatcac cgaccggcgg cgtcttgatg ttgtcgagga
      781 atgacagcgg gtagctgccg cgcgagttcg gggtcagtcg ctcgtcgtcg acgtcgaact
      841 cgccgtccgg gtacatcaga caattctcga cgatcgcgcc gtgctgcagc ggatcgacct
      901 cgtgaaagac ggcgcggtag atctcggctt ccttctcctc acgcaggttg atcagcttgg
      961 cgtagcagcc gccctcgaag ttggccactc cgcggtcgcc ccacccgtgc tcgtcatcgc
     1021 ccaacaacgc gcgatcggga tcggccgaca ggctggtctt gccggttccg gacagtccga
     1081 gcagcaacgc ggtgtctccc cgccgaccct cattcgccga acagtggagc gggaggatgc
     1141 cgtgttccgg cagcaggaag ttcatcacgg tgaacatcag cttcttgatg ctgccgcagt
     1201 aggccgaacc gtagaccagg ccgagccgcc ggtcgaagtc catcgcgatg accatgtccg
     1261 acgtggtgcc gtcgccggtc ttgcgcagac gtccggcgta gcgcgacgtc tcgatcttgt
     1321 cgtacggcaa cgccacgatc tggaagccgt agtcggcgaa gatgctgttc tccgcgccgt
     1381 ccggcctggc gcggaacatg ttgtcgacga agacgctgtg aagcgcgcga tcggtgatgg
     1441 tcttcaccgg cagggcatag gccgggtcgg cgccgaggta gcggttggtc acgtacagcc
     1501 gcggcttgcc ggccagtact tcgagcgcat cgctccacaa catctcgaac gtctcgggat
     1561 cgagcgggat gcagttcggc gagtcccagt cgattttctt ctcgctctgc gggttgcgaa
     1621 cgatgacggt gtccttcggg ctgcggccgg tcgagtcgga cggtgtccag gtggccagcg
     1681 caccgcagcg ggacgcgaag gcctccttgt tttccagggc gtgacgaatc aactcctgtc
     1741 gaccgagatc gacacattta cgccgatgct cgtcgatcat tcgctcgagt gccgtggtaa
     1801 acgccgcttc gttcgtgatc gccatggttc atctccccat ttgcgggctc ggtcgttcga
     1861 ccgacatgtc gggattatcc cgcgtcgccg cccggggctc cagggcggtc tcgcgcgaca
     1921 gtggtccaaa ccggtaagga attcccccac gctccgcgtc cgttcggccc ggtcccgtcc
     1981 gtcgcacgat cgggtcacga taccggggct gccgcgacgc gtcaaacggc tacccgggcc
     2041 ggtcgcgcgc gccgtggcgt cgtggcaacc gatcctgtta atctacggct tctccgcgag
     2101 aaagcgacgg acgggcaccg tccgatcgac cgcggggcgg gggaatcgga cgcatgggtc
     2161 agccagagat cggccgggtg aggatcgcga ccgccggcgt gctgttggcg gccaccgccg
     2221 cctgggccga ggggctttac gacatgccga tcggcgatct cggccgcgcg cagcggacgg
     2281 tcgagctggt gctcgacgcc gtcaccgact gccataccgg cgaggtcatc cctcccggcg
     2341 agctgccccg gcggctcgac ggcgtccgcc tgctgttcgt cggcgaacgg cacatcagcg
     2401 ccgagtttca tgcggtcgag ctccgcgtcc tgcaggagct cgtgcgcgcg ggtcggaccg
     2461 tggcgatcgg cctcgagatg tacccgtaca ccgaacaacg ctacctcgac caatggtcgg
     2521 cggggttgct gaccgaacag gggttcgtcg agctgtcgga ctggtacgac aactggggct
     2581 acgactggca gtactatcgt gagatcttcc tgttcgcgcg cgaccacggc ttgccgatgc
     2641 acgcgctcaa caccccacgg gaggtgatca gcgcggtgcg tcagaagggg ttcgacgacc
     2701 tgacggagga ggaggcgcgc cacgtcccgc cggcgatcga caccgacagc gacgaccacc
     2761 ttcgactgtt ccgcgcgatg ctcgaggagg acagcgacga ggactttcac gccgagctgc
     2821 cggacgagat cttgcggccg atgttcgacg cgcagtgtac ctgggatgcg acgatggccc
     2881 atcacgcggt caaggcgctc gccggcggcc aggccgatga gatcttggtc gtgctcgtcg
     2941 gcaccggtca tctggcctac gagctcggcc tgcaacggca ggcacggcag tggttcgacg
     3001 gccggatggc gacgttgatc ccgatctcgg tcggcgacga gtgcgagccc gttcatcgcg
     3061 tccgcgcgtc gtttgccgat ttcgtctggg gcgtgcccga ggaaccgtcg ccggcccgtc
     3121 cgttcctcgg tctgtcgacg acggccgacc cggaaagcgg caggcgcaag gtgatctacg
     3181 tttcgaccga ttcccccgcc gaggcggcgg gcttcgccgt cggcgatctg gtgctgacac
     3241 tcgacggcgt cgagctcgcg gaccaggcgt cgctgaagcg tctcacctcc gccaagcagt
     3301 ggggagacac ggcacgcttc accgtcgcac gcggcggcga gacgatcggt ctcgacctcc
     3361 ggtttcgccg aaatcgcccc gagccgtgcg agggcgacga gtgatgcggg tcgtggcggc
     3421 gggggtcgtc accgcggttc tggccgggcc ggggctcggc gccgcggcgg cgcccgtcga
     3481 gcacgagatc gaactcgcgc tcgagccgca gagcggacga ttgaccgtcc gcgatcgggt
     3541 gaccgtgcgg gtcggtgcga cgacgggatc ggtcgtcgag ttcgtgttga acgagcgcct
     3601 cgagctcgat cccgccgcac cggtcgagcc ggtgggggag ccgttcagcg cggcgcgatt
     3661 cgagctcaac gggcaccaga tcgcgcgcgg gggcgaggcc acgattcgac gctaccgctt
     3721 gaccgatccg ctcgatcgag ggccgttcac catcgcctac accggagcga tggcgttcga
     3781 gctgggcgag gtgcacgagg agtacacgcg cggcttccgc gagacgatcg gtcagctcgg
     3841 ccccgacggg gtctacctca ccgccgacag cttctggtac ccctacttcg gcgacggcct
     3901 ggtcgagtat cggatgacgg tcgagcagcc cgacgggtgg cacgtcatct cggccggtcg
     3961 cggccggtcg cgcgacgacg acgaccgcgc gcagtgggag tccgagggtg cggtcgacga
     4021 gatctacctg gccggtggtc cgttgtatct ccaccgcgac tccgtcggcg ccgtcgagac
     4081 cctggtctat ctccacgagg acgatcccgc gctgtccgag cgctacctga cggcgaccgc
     4141 gcagtacctc gagatgtacc gctcgatgat cggcccgtac gcctacagca agttcgcgct
     4201 ggtcgagaac ttctgggaga caggctacgg catgccgtcg ttcaccttgc tcggtccgcg
     4261 cgtgatccgg ctcccgttca tcctccactc gtcctacccc cacgagatcc tgcacaactg
     4321 gtggggcaac ggcgttttcg tcgactacga ctccggcaac tggtgtgagg gcctgacggc
     4381 ctacctcgcc gatcacctgg tccaggagca acgcggccag ggcgccgcct accggcggga
     4441 cacgctgcag aagtaccgcg attacgtcgg tgagaaccgc gactttccgc tcgagcagtt
     4501 tcgcacgcga cacagtgccg cgaccgaagc ggtcggctac ggcaagtcgc tcgtgggctt
     4561 ccacatgctg cgcatggcga tgggcgacga cgtctttcgc cgcgcgttgg ccgagctcta
     4621 ccgcgagtac cgcggtcgcc gggcgtcgtt cgacgacctg cggcgcgtgt tcgagtcggc
     4681 atcggggctc ccgctcgccg acttcttccg gcagtggctg acacgcaccg gggcgccggc
     4741 gctcgcagcc cgtgtcgcat ggtgcgacgc gggcacgacg ctacgggtct gcgggacgct
     4801 cgagcagacc cagagcgggc cgccgttcgc ggtcgacgtc ccgatcgtgc tcgacacggc
     4861 ggacggtccg caagagcaca cggtccgcat gaagacgcga gagcagggat tcgcgctcgc
     4921 ggcgaccacg cccgtctggc gcgtgcgcgt cgatccgtcg ttcgacgtgc tgcgtctgct
     4981 cgacccgcgc gaaacgcccc cgacgatcgg ccagctgttc ggcgagccga ggatcctggc
     5041 ggtgctgccg tcgagcgacg cgccgcgccg ggaggcctac cgcgcgctgg tcgagggctg
     5101 gcggagcgct tcgcacgaga tcgaggtcgt ggtcgacgcg gagctggccg cgctgcccgc
     5161 cggcctgtcg gtctgggtcc tcgggcggca aaaccggttc gccgggccgc tgttcggtgg
     5221 cggcgtcccc cgcggcttcg agctgttgcc gacctcgacg gcgttcgggc agcaatccgt
     5281 gtcgtccgcc ggccactcga tcgtttcggt cagacgccat cccgcgagcg agcgccacgc
     5341 ggtcgggttg ctcgacgtcg atcccgaggc ggccttcccc ggtatcggtc gcaagttgcc
     5401 gcactatggc aagtactcgt acctggcgtt cgccggggac gagcctgaga acgtgatcaa
     5461 ggggcagtgg ccgacggacg actccccgct cgtcgtcgag ctggcggcgc cgccggaccc
     5521 cgtcggcgcc gatcccggcg ccgctccggc cgagcgcagc gcgctcgccg agctgccgcc
     5581 gctcttctcg cggcaggccc tcggcgacca cgtcgactac ctcgcgtcgc gcgagctcga
     5641 agggcgcggt gtcgggacga ccgggctcga tcgggccgcg gagtacgtcg ccgcgcgctt
     5701 cgccgcggtg ggcctcgagc cggcggggga
//
LOCUS       DASPGN010000117         5221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_255828, whole genome shotgun sequence.
ACCESSION   DASPGN010000117 DASPGN010000000
VERSION     DASPGN010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255828"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VD788_03250"
     CDS             <1..725
                     /locus_tag="VD788_03250"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75309.1"
                     /translation="DDGEEREYAIPKGMHVTVQEGEAVRAGDAFTDGPKDPHKILEIL
                     GERELQNYLLDGIQEVYRLQGVNIHDKHVEVIVRQMMRWIKVEDPGDTDFIVDEQVDR
                     FRFVKENERVLEEGGAPARGRPLLLGITKASLATESFISAASFQETTRVLTEASISGK
                     VDYLRGLKENVIMGRLIPAGTGMETYTELEIAPDGPPPVEEEFAEEALEAEVDLDTDR
                     FAEMIRQAVAASSGSDSGSKSA"
     gene            923..1297
                     /gene="rpsL"
                     /locus_tag="VD788_03255"
     CDS             923..1297
                     /gene="rpsL"
                     /locus_tag="VD788_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HXV75310.1"
                     /translation="MPTVSQLVRNGRAMIRARTKSPALNSCPQKRGVCVRVYTSTPKK
                     PNSALRKVARVRLTNGIEVTTYIPGVGHNLQEHSIVMIRGGRVKDLPGVRYHVIRGTL
                     DAVGVEGRRKSRSKYGAKRPKG"
     gene            1308..1778
                     /gene="rpsG"
                     /locus_tag="VD788_03260"
     CDS             1308..1778
                     /gene="rpsG"
                     /locus_tag="VD788_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXV75311.1"
                     /translation="MPRRAQIPKRTILPDPIYQSPLVTRFINSLMLSGKKSIAEGTFY
                     GAMDTIKERTSDDPLRVFRKAVDNVKPALEVKSRRVGGSNYQVPVEVRPQRRTALAIR
                     WLIAFARSRAEKSMREKLANELMDAAGSRGAAFKKKEDTHRMAEANKAFAHYRW"
     gene            1892..3991
                     /gene="fusA"
                     /locus_tag="VD788_03265"
     CDS             1892..3991
                     /gene="fusA"
                     /locus_tag="VD788_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXV75312.1"
                     /translation="MDRQTPLERIRNIGIMAHIDAGKTTTTERILFYTGINYKVGEVH
                     EGTATMDWMAQEQERGITITSAATTCFWRDHRVNIIDTPGHVDFTAEVERSLRVLDGA
                     VAVFCAVGGVEPQSEAVWRQADRYRVPRIALVNKMDRVGADFARVCTMMERRLQARPL
                     PVQLPLFAEGEPGEFVGLVDLIERRALVWGDDLIGSPYDVQPVPARMEREVEAARERL
                     LECLSEFDDRLLEQYLGGRTIDPGELKAAVRRGTRELRFTPVLCGSAFRNKGVRPLLD
                     AIVDYLPSPLDVPPIEGVDPRNGDALTRRADDGAPLSALVFKIMTDPFVGHLAFIRVY
                     SGVLDAGAHVFNATRGRRERVGRLLKMHANKREEIRRLFSGDIAAVVGLKQATTGDTI
                     CAEREPVLLEAMEFPEPVIRMAIEPKTKADQDRLGSALAKLAQEDPTFRVTTDPDTGQ
                     TLIAGMGELHLEVLVDRLRREFSVSARVGKPQVAYRETIHRPGDAESRYVKQTGGRGQ
                     YGHVKLRVRPSGPDVGRTFASAIVGGAVPKEFVPAVEAGVREVMDSGILAGFPMCDVA
                     VELYDGSYHEVDSSDLAFKVAGAMAFREACRRAGVVLLEPVMRIDVVVPDEYMGEVIG
                     DLNARRGRVQTIESRPGLSVIRSSVPMAQMFGYSTDLRSLTQGRGSYTMHFERYEEAP
                     KAVRDEVLARVSGTTPS"
     gene            4034..5221
                     /gene="tuf"
                     /locus_tag="VD788_03270"
     CDS             4034..5221
                     /gene="tuf"
                     /locus_tag="VD788_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HXV75313.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHVDHGKTTLTAAITSVLAKGNPKIK
                     ARSFDSIDNAPEERERGITIATAHVEYETSKRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVSAADGPMPQTREHILLARQVGVPAIVVAMNKVDMVDDPELLDLVELEVRELLS
                     QYEFPGDDIPIVRISALKAMEGDAAYEKTIGELMEQVDTYIPLPQRAVDKDFLMPVED
                     IFSISGRGTVVTGRIEQGRVKVGEEVEIVGIRPTQKKVVTGVEMFKKLLDAGEAGDNV
                     GLLLRGTERKDVERGQVLAKPGSITPHTKFMGEVYVLTKEEGGRHTPFFTGYRPQFYF
                     RTTDVTGTATLPDGVEMVMPGDNVTIEVNLITPIAMDKGLRFAIREGGRTVGAGTVTE
                     IVE"
BASE COUNT          934 a         1539 c         1909 g          839 t
ORIGIN      
        1 ccgacgacgg cgaggagcgc gagtacgcga ttccgaaggg catgcacgtg acggtgcagg
       61 agggagaggc ggttcgtgcc ggcgacgcgt ttaccgacgg cccgaaggac ccgcacaaga
      121 tcctcgagat cctcggcgag cgcgaactcc agaactacct gctcgacggc attcaggagg
      181 tctaccggct ccagggcgtg aacatccacg acaagcacgt cgaggtgatc gttcgccaga
      241 tgatgcgctg gatcaaggtc gaggatccgg gagacacgga tttcatcgtc gacgagcagg
      301 tcgaccgctt ccgcttcgtc aaggagaacg agcgggtgct cgaagaggga ggcgcaccgg
      361 cccgcggtcg tccgttgctg ttgggcatca ccaaggcgtc actggcgacc gagtcgttca
      421 tctcggccgc gagcttccag gaaacgacgc gcgtgctgac cgaggcgtcg atctcgggca
      481 aggtcgatta cctgcgcgga ctcaaggaga acgtgatcat gggtcggctg attccggccg
      541 ggaccggcat ggagacttac accgagctcg agatcgcgcc cgacggtccg ccgccggtcg
      601 aggaggagtt cgccgaggaa gcgctcgagg ccgaggtcga tctcgatacg gatcgcttcg
      661 ccgagatgat ccgccaagcg gtggccgcgt cgtccggaag cgattccgga agcaagtcgg
      721 cctgagcttg cgacgggcgg tcttgaccgt tttcgcgggg ctctgctaga ctctcggggt
      781 ttggcgtcag gatggcgggc atgccggacc ggggcggccg atcgggctcg agctccggga
      841 tcggagaagc gtgccggcgc gggtaggtac ggcgcaacaa cacggttgct ctttcggtcc
      901 gggcgccacg gaaggaatcg acgtgccgac agtcagtcag ttggtccgca acggccgggc
      961 gatgatccgg gcgcgaacga agagcccggc gttgaactcc tgcccgcaga agcgcggtgt
     1021 ctgcgtccgg gtctacacct cgacgccgaa aaagccgaac tccgcgctgc gcaaggtggc
     1081 gcgcgtccga ctgaccaacg ggatcgaggt gacgacctac attcccgggg tcggacacaa
     1141 cctgcaggag cactcgatcg tgatgatccg cggggggcgc gtcaaggacc tccccggcgt
     1201 tcgctatcac gtgatccggg gcacgctcga cgccgtcggt gtcgaggggc ggcgcaagag
     1261 ccgctcgaag tacggcgcga agcgaccgaa gggctaggac gaggaccatg ccgcgacgcg
     1321 cacagattcc caagcgaacg atcctgcccg atccgatcta ccagtctccg ctggtgacgc
     1381 gattcatcaa cagcctgatg ctgtcgggca agaagtcgat cgccgaaggc acgttctacg
     1441 gagcgatgga tacgatcaaa gagcgcactt cggacgatcc gctgcgggtc ttccgcaagg
     1501 cggtcgacaa cgtcaaaccg gcgctcgagg tcaaatcgcg tcgcgtcggc ggctcgaact
     1561 accaagtgcc ggtcgaggtg cggccgcagc gtcgcaccgc gctcgcgatt cgctggctga
     1621 tcgccttcgc tcgctcgcgt gcggagaagt cgatgcggga gaagctcgca aacgaattga
     1681 tggacgccgc cggctcgcgc ggagcggcgt tcaagaaaaa ggaagatacg caccggatgg
     1741 ccgaagccaa caaggcgttc gcgcactacc ggtggtgagc cggccccgat cggacacggg
     1801 caaccggcag gaatcgtctt gaccgcgccc acagggcgcg ggcggtaggg gcgggtcgcg
     1861 ccgagacgcg tgagccgcgt ggttctttga catggatcgg cagacgcctc tggagaggat
     1921 ccggaacatc gggatcatgg cccacatcga cgccggaaaa acgacgacga ccgagcggat
     1981 cctgttctat accgggatca actacaaggt cggcgaggtc cacgagggga ctgccaccat
     2041 ggactggatg gcgcaggaac aggagcgcgg catcacgatc acctccgccg cgacgacctg
     2101 tttctggcgc gatcatcgcg tcaacatcat cgacaccccg ggacacgtcg acttcaccgc
     2161 cgaggtcgag cggtcgctgc gcgtgctgga cggtgccgtc gccgtctttt gcgccgtcgg
     2221 aggtgtcgag ccgcagtccg aggcggtctg gcgtcaggcc gatcgctacc gggtcccgcg
     2281 tatcgcgctg gtgaacaaga tggaccgcgt cggcgccgac ttcgcgcgtg tctgcacgat
     2341 gatggagcgg cgcctgcagg cccggccgct gccggttcag ctgccgctgt tcgccgaggg
     2401 cgagccgggc gagttcgtcg gactcgtcga cctgatcgag cgccgggccc tcgtctgggg
     2461 cgacgatctg atcggctctc cctacgacgt gcaaccggtt cccgcgcgca tggagcgcga
     2521 ggtcgaggcg gcgcgcgagc ggctgctcga gtgcctctct gagttcgatg accgattgct
     2581 cgagcagtac ctcggcggcc ggacgatcga tccgggcgag ttgaaggccg cggtgcggcg
     2641 cgggacccgc gagctgcgat tcaccccggt gctgtgtggc agcgcctttc gcaacaaggg
     2701 ggttcgacct ctgctcgacg cgatcgtcga ctacctgccc tcgccgctcg acgtgccgcc
     2761 gatcgagggc gtcgacccgc ggaacggtga cgccctgact cgccgcgcgg acgatggcgc
     2821 tccgttgtcg gcgctggtgt tcaagatcat gaccgacccg ttcgtcgggc acctggcctt
     2881 catccgcgtc tactccggcg tgctcgacgc cggcgcgcac gtgttcaacg caacgcgcgg
     2941 ccgccgagag cgcgtcggtc ggctgttgaa gatgcacgcc aacaagcgcg aggagatccg
     3001 gcggttgttc tccggcgaca tcgccgcagt cgtcggactg aagcaggcga cgaccggcga
     3061 cacgatctgc gccgagcgcg agccggtgct gctcgaggcg atggaattcc cggaaccggt
     3121 gattcgcatg gcgatcgagc ccaagaccaa ggccgatcag gaccgcctgg gctcggcgct
     3181 ggccaagctg gcccaggaag acccgacctt ccgcgtgacg accgaccccg ataccggcca
     3241 gacgctgatc gcggggatgg gcgagctgca cctcgaggtc ctggtcgacc gcctgcgtcg
     3301 cgagttttcc gtgtcggcgc gcgtcggcaa gccgcaggtg gcctaccgcg agacgatcca
     3361 ccggccgggg gatgccgagt cgcgctacgt caagcagacc ggcgggcgcg ggcagtacgg
     3421 ccacgtcaag ttgcgcgtgc ggccgtcggg tccggacgtc ggacggactt tcgccagcgc
     3481 gatcgtcggc ggggcggtcc cgaaggagtt cgtcccggcc gtcgaggcgg gcgtgcgcga
     3541 agtgatggac agcggcatcc tcgccggttt tccgatgtgc gacgtcgcgg tcgagttgta
     3601 cgacggttcc taccacgagg tcgattcgtc ggatctggcg ttcaaggtcg cgggggcgat
     3661 ggccttccgc gaggcttgcc gccgggccgg cgtggtgcta ctcgaaccgg tgatgcggat
     3721 cgacgtcgtc gtaccggacg agtacatggg cgaggtgatc ggcgatctga acgctcgccg
     3781 cggacgggtt cagacgatcg agtcccggcc cggattgagc gtgattcgtt cgtccgtgcc
     3841 gatggcgcag atgttcggct actcgaccga cctgcgctcg ttgacccagg gtcggggaag
     3901 ctacacgatg cactttgaac gctacgaaga ggctccgaag gcggtccgcg acgaggtgct
     3961 ggcccgggtt tcgggcacca ccccgtcgtg agcgtgcgac agccggacga aacccagcag
     4021 gaggagagcg gcgatggcca aggagaagtt cgatcgatcg aagccgcacg tgaacgtggg
     4081 gacgattggt cacgtggatc acgggaagac gacgttgacg gcggcgatca cgtcggtgtt
     4141 ggccaagggc aatccgaaga tcaaggcccg gtcgttcgat tcgatcgaca acgcgccgga
     4201 ggagcgtgag cgtgggatca cgattgcgac ggcgcacgtg gagtacgaga cgagcaagcg
     4261 gcactacgcg cacgtggatt gtccgggtca cgcggactac atcaagaaca tgatcacggg
     4321 tgcggcgcag atggatggtg cgattttggt ggtgtctgcg gcggatggtc cgatgccgca
     4381 gactcgcgag cacattttgt tggcgcgaca ggtgggtgtg ccggcgatcg tggtggcgat
     4441 gaacaaggtg gacatggtcg acgacccgga gttgttggat ttggtggagc tcgaggttcg
     4501 ggagttgctg agccagtacg agtttcctgg ggacgacatt ccgatcgtgc ggatatcggc
     4561 gttgaaggcg atggaggggg atgcggcgta cgagaagacg atcggggagt tgatggagca
     4621 ggtggacacg tacatcccgt tgccgcagcg agcggtggac aaggattttc tgatgccggt
     4681 cgaggacatc ttctcgatat cgggtcgcgg gacggtggtg acgggtcgga tcgagcaggg
     4741 tcgggtgaag gtgggtgagg aagtggagat cgtcggcatc cgtcccacgc agaagaaggt
     4801 ggtgacgggt gtggagatgt tcaagaagct gttggacgcg ggcgaggcgg gggacaacgt
     4861 cgggttgttg ctgcgcggga cggagcgcaa ggacgtggag cggggtcagg tgctggcgaa
     4921 gccgggttcg atcacgccgc acacgaagtt catgggcgaa gtctacgtgt tgacgaagga
     4981 agagggcgga cgtcacacgc cgtttttcac cgggtatcgc ccgcagttct acttccgcac
     5041 gacggacgtg acggggacgg cgacgctgcc ggacggcgtg gagatggtga tgcccgggga
     5101 caacgtgacg atcgaggtga acctgatcac gccgatcgcg atggacaagg gcctgcggtt
     5161 cgcgatccgc gagggcgggc ggacggtcgg cgccgggacc gtcaccgaga tcgtcgagta
     5221 g
//
LOCUS       DASPGN010000118         4901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1543170, whole genome shotgun sequence.
ACCESSION   DASPGN010000118 DASPGN010000000
VERSION     DASPGN010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543170"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VD788_03275"
     CDS             <1..137
                     /locus_tag="VD788_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HXV75314.1"
                     /translation="LKATIRDYIRTNNEDPKPFVWTKTADEILASVARFCQRTSDSGH
                     "
     gene            complement(134..997)
                     /locus_tag="VD788_03280"
     CDS             complement(134..997)
                     /locus_tag="VD788_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXV75315.1"
                     /translation="MRAKAHSYRFGAFAFDADSGELRRLDSAAPPARLPPQPARLLAL
                     LADKNGEVVTREEIRERIWSGVEVEFDTSLHFCIRQIRAALGDSASGSRYVETLPRRG
                     YRLIPEVETSSPAGATPADRGRRGWWRAGGATVLVGAAAAAALLLSGGRSGPANADPL
                     RIAVMPFRPPAVTPFGEIPPIAEWIVEDLTHTAGPRAAIIGPTTTTGYDGTDAAIARL
                     AADYDLDYVVNGRFLDDGRGARMLAELIRVSDGAHTWVEAYHELDDGRRIGREISAGV
                     VRELGLGRSGR"
     gene            complement(1048..2490)
                     /locus_tag="VD788_03285"
     CDS             complement(1048..2490)
                     /locus_tag="VD788_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXV75316.1"
                     /translation="MQDRRRGFLKASIMAAGGAALGASVRARSGVAGAAGDDPVPPTR
                     RFVEAVAAGDEEAVRRWLDRDPGLIHARDDAGRTAFAIALIHRHPGIAGLLRDRGYPP
                     DLHESALAVDWERFERLAAATPGAVNQDHPVGGTAMYAAAVGGAGSQIWRVYRFGGEP
                     DLRGASGYSPLRAALEHPDLDVAELTASGLLGNGADPAAEQADGSSPLHVAARRGSTA
                     IVELLIRKGADVEALDRGGRTARRLADEAGRAEVVELLDSHRTIPRDHSTSREAYDVA
                     GRAYRRPDLSAFSIGERSRVVGVAHGGFDDLRQAVNRHPELAHAISTTTESAVEAGAH
                     TGHADIVDLLIEHGAAYSLPTAVMRGDLARARALLDEDPLRIHERGPHDFALLWYPVI
                     GQTSTEMLELLLARGAEVERQHVLGTTALHFASLRGAVEMAELLIAHGADVNRPGRRF
                     DATPETPLQLAEARGHQELVRLLRAHGARG"
     gene            2618..2755
                     /locus_tag="VD788_03290"
     CDS             2618..2755
                     /locus_tag="VD788_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75317.1"
                     /translation="MLPVAAGAWDCKTRFANSAGETAEGRAWIEGVGHVEAGRVARSG
                     G"
     gene            complement(2752..3957)
                     /locus_tag="VD788_03295"
     CDS             complement(2752..3957)
                     /locus_tag="VD788_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV75318.1"
                     /translation="MLCLVVLTVAASGAVAAERLAVRAARVHPVSAAVIEDGVVLIED
                     GKIAAVGPASRVRVPDGVEVIEAETVTPGLIDVHTVVGLAGIYNSDRGQVQDQDQLEK
                     SDPVQPQLRAIDAYDAVEPLVAWVRSLGVTTVHTGHGPGAVVSGQTLIAKTRGATVEE
                     AVIVPAAAVAATLTEEVSRNFDSPGTHAKTTAALREALVTAAAYREARQADKPVDRDL
                     AKETMARVLDGELALMVTAQTATEIATALRLRREFGFTLWLDGAAEAYRMIDPIREAG
                     VPVLLHPTMMRAGRETRNAAFDTAVKLAEAGIPFAIQSGFEDYVPKTRVLLYEAQVAV
                     SHGLDPDQALAAITLAPAEILGLDRRLGSIEVGKDADLVLFDGDPFEYTSHVCRVIID
                     GLTVAEECR"
     gene            complement(3984..>4901)
                     /locus_tag="VD788_03300"
     CDS             complement(3984..>4901)
                     /locus_tag="VD788_03300"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV75319.1"
                     /translation="GSGHLMSGQTVYLKLRRDPATIEDWLVCDDPIDGVCGSMKMANG
                     TNSMRDKPFPGTRAKSAAMVRDLFVRAQDYVAKRQQAAAGKAEDGKAPDRDLELEAVA
                     QILDGRRRVQHHTHRHDDIATVLRLQREFGFDVVIQHGTETWMLADELAEAGIGVSFT
                     LVDSPGGKEEILRMRMDAPALLERAGVDVSLNTDDWITDSRLFLRTAAMAHRHGMTRE
                     GALEAVTLAPARQLGLDRRVGSLEPGKDADLVVLSGDPLSVYTRVLETWVEGERVYDA
                     SDPDHAKYARGGWQVYRGSSDHVHDGCWR"
BASE COUNT          712 a         1787 c         1702 g          700 t
ORIGIN      
        1 agctgaaggc cacgatccgc gactacattc gcacgaacaa cgaagatccc aagccatttg
       61 tctggacgaa gactgctgat gaaatcctgg catccgtcgc ccgattttgt cagcgaactt
      121 cggactcagg acactagcgg ccgctccggc cgagccccag ctcgcgcacg acccccgcgc
      181 tgatctcgcg gccgatgcgc cgtccgtcgt cgagctcgtg ataggcctcg acccaggtgt
      241 gggcgccgtc cgacacccgt atcagctcgg cgagcatccg cgcaccccgg ccgtcgtcga
      301 ggaagcggcc gttgacgacg tagtccagat cgtagtcggc ggccagccgc gcgatcgccg
      361 cgtcggtgcc gtcgtacccc gtcgtagtgg tcggcccgat gatcgcggcg cgcgggccgg
      421 cggtgtgcgt caggtcttcg acgatccact cggcgatcgg cgggatctcg ccgaacggcg
      481 tcacggccgg tggccggaac ggcatgaccg cgatccgcaa cggatcggcg ttcgccggcc
      541 ccgagcgacc cccgctcaac agcagcgccg ccgccgccgc cgcgccgacg aggacggtcg
      601 ccccgcccgc gcgccaccag ccgcgccgcc cgcggtcagc cggcgtcgcc cccgcgggcg
      661 aactggtctc cacttccgga atcagccggt agccgcgccg cggcagcgtc tcgacgtagc
      721 gcgacccgct cgccgagtcg ccgagcgcgg cccggatctg ccggatgcag aagtgcaggc
      781 tggtgtcgaa ctcgacctcg acaccggacc agatgcgttc gcggatctcc tcgcgcgtga
      841 cgacctcgcc gttcttgtcg gcgagcaggg ccaacaggcg ggccggctgg ggcggcagcc
      901 gtgcgggagg agccgccgag tcgagccggc gcagctcacc gctgtccgcg tcgaacgcga
      961 acgcgccgaa tcgatagctg tgagccttgg cgcgcacgtg ccgagcagca taagggtcgc
     1021 ccgggaggcg ggcaagcaca ccggcgctca gcctcgcgcc ccgtgggcgc gaagcagccg
     1081 caccagctct tgatgaccgc gggcttcggc cagttgcagc ggcgtctcgg gcgtcgcgtc
     1141 gaaccggcgt cccggccgat tgacgtccgc cccgtgcgcg atcaacagct cggccatctc
     1201 gaccgcaccg cggagcgaag cgaagtgcag cgcggtcgtt cccaggacgt gctgccgttc
     1261 gacctccgcg ccccgtgcca acagcagctc caacatctcg gtcgacgtct ggccgatcac
     1321 cgggtaccac aacagcgcga agtcgtgcgg gccgcgctcg tggatccgca gcgggtcctc
     1381 gtcgagcaac gcccgggcac gcgccaggtc gcctcgcatc accgcggtcg gcaacgagta
     1441 ggccgcgccg tgctcgatca gcaagtcgac gatgtcggcg tgtccggtgt gggcaccggc
     1501 ctcgaccgcc gactcggtgg tcgtcgagat cgcgtgggcg agctcggggt ggcggttgac
     1561 ggcctggcgc aggtcgtcga acccgccgtg cgccaccccg acgacgcgcg accgctcgcc
     1621 gatcgaaaag gccgacaggt cgggccggcg ataggcgcga ccggcgacgt cgtacgcctc
     1681 gcgcgaggtc gagtggtcgc gcgggatcgt tcgatggctg tcgagcaact cgacgacctc
     1741 cgctcggccg gcctcatcgg ccagccggcg cgcggttcgg ccaccgcgat ccagcgcctc
     1801 gacgtcggct cccttacgga tcaacagctc gacgatcgcc gtgctcccgc gacgggcggc
     1861 gacgtggagc ggcgagctgc cgtcggcctg ttcggccgcg ggatcggccc cgttgccgag
     1921 caacccggac gccgtcagct cggcgacgtc cagatcgggg tgctcgagcg ccgcgcgcaa
     1981 cgggctgtag ccgctcgccc cgcggaggtc cggctcgccg ccgaagcgat agacccgcca
     2041 gatctgactg cccgcgcccc cgaccgcggc cgcgtacatc gcggtgccgc cgaccggatg
     2101 gtcctggttg accgcgcccg gcgtcgcggc ggccaaccgc tcgaagcgct cccaatcgac
     2161 cgccagcgcg gactcgtgca gatcgggcgg atagccacgg tcgcgcagga gaccggcgat
     2221 ccccggatga cggtggatca gcgcgatcgc gaaggccgtc cgtcccgcgt cgtcacgcgc
     2281 gtggatcaga ccgggatcgc ggtcgagcca acggcggacg gcctcctcgt cgcctgccgc
     2341 caccgcctcg acgaaccgcc gcgtcggcgg gaccgggtcg tcgccggcgg cgcccgcgac
     2401 cccgctgcgc gcgcgcaccg atgcgccgag ggcggcgcca ccggccgcca tgatcgaagc
     2461 cttgagaaaa ccgcgtcgtc gatcctgcat cgcccacctc gccgacggct gtgatgccgt
     2521 gatgcagtcg agcgtattgg gcggcgagcg agtcggccac ggagaatgcc ttagaaaagc
     2581 cttagcgcgc gatgcatgtc gctctcatga gaacgcggtg ctgccggtcg cggcgggcgc
     2641 gtgggattgc aagacccgat tcgccaattc cgccggtgag accgccgagg gccgcgcgtg
     2701 gatcgagggc gtgggtcacg tcgaggccgg gcgggtcgct cggtccggcg gctagcgaca
     2761 ttcctcggcg acggtgaggc cgtcgatgat cacgcgacag acgtgcgagg tgtactcgaa
     2821 cgggtcgccg tcgaaaagca cgaggtcggc atccttgccc acctcgatcg agccgaggcg
     2881 acggtcgaga ccgagaatct ccgcgggggc cagcgtgatc gcggccagcg cttggtcggg
     2941 gtccaagccg tggctgacgg cgacctgcgc ttcgtacagc agcacgcggg tcttgggtac
     3001 gtagtcctcg aacccgctct ggatggcgaa cgggatgccc gcctcggcca acttgaccgc
     3061 cgtgtcgaag gccgcgttcc gcgtctcccg cccggcacgc atcatcgtcg gatggagcag
     3121 caccggcacg ccggcctcgc ggatcggatc gatcatgcgg tacgcctcgg ccgcgccgtc
     3181 gagccacaac gtgaaaccga actcgcgccg cagccgcagc gcggtcgcga tctcggtcgc
     3241 cgtctgcgcg gtgaccatca gcgccaactc gccgtcgagc acgcgcgcca tcgtctcctt
     3301 cgccagatcg cgatcgacgg gtttgtcggc ctggcgtgcc tcgcggtagg cggcggccgt
     3361 gaccagcgcc tcgcgcagcg cagcggtggt cttggcgtgg gtacccggag agtcgaagtt
     3421 gcgcgacacc tcctcggtca gcgtcgcggc gacggcggca gcgggcacga tcaccgcttc
     3481 ttcgacggtc gcgccacggg tcttggcgat cagcgtctgt ccggagacga cggcacccgg
     3541 tccgtggccg gtatggaccg tcgtcacccc gagcgagcgg acccaggcga cgagcggctc
     3601 gaccgcgtcg taggcatcga tcgcgcggag ctgcggctgc acgggatcgg acttctccag
     3661 ctggtcctgg tcctgcacct ggccgcggtc tgagttgtag atccccgcca gaccgaccac
     3721 cgtgtggacg tcgatcaagc ccggggtgac ggtctcggcc tcgatcactt cgacgccgtc
     3781 cgggacccgc acccgcgagg ccggtccgac ggcggcgatc ttcccgtcct cgatcagcac
     3841 cacgccgtcc tcgatcaccg cggccgagac cgggtggacg cgcgccgccc gcacggcgag
     3901 gcgctcggcg gcgaccgcgc cgctcgcggc caccgtcagg acgacgaggc agagcaaacg
     3961 attcgccggc ctgcgcggcg cgctcaccgc cagcacccgt cgtggacgtg gtcggacgag
     4021 ccgcggtaaa cctgccagcc gccccgcgcg tacttcgcgt ggtccggatc ggaggcgtcg
     4081 tagacccgct cgccctcgac ccaggtttcg agcacgcgtg tgtagacgct gagcggatcg
     4141 cccgacagca cgaccaggtc ggcgtccttg cccggctcga gcgagccgac acgtcggtcg
     4201 aggccgagct ggcgcgccgg cgcgagcgtc accgcctcga gcgcgccctc gcgcgtcatc
     4261 ccgtgccggt gcgccatcgc ggcggtgcgg aggaacagac gcgaatcggt gatccagtcg
     4321 tcggtgttga ggctgacgtc gacgccggcc cgttcgagca gggccggcgc gtccatccgc
     4381 atccgcagga tctcctcctt gcctcccggc gaatcgacga gcgtgaagga gacgccgatg
     4441 ccggcctcgg ccagctcgtc cgccagcatc cacgtttcgg tcccgtgctg gatcacgacg
     4501 tcgaagccga actcgcgctg caaccgcagc accgtcgcga tgtcgtcgtg gcggtgggtg
     4561 tggtgctgga cgcgacgcct gccgtcgagg atctgtgcca ccgcctcgag ctccagatcg
     4621 cggtcgggag ccttcccgtc ttcggctttc ccggcagccg cctgctgccg cttggccacg
     4681 taatcctgcg cgcgcacgaa cagatcgcgc accatcgcgg ccgatttcgc gcgggtcccc
     4741 ggaaacggtt tgtcgcgcat cgaattcgtc ccgttggcca tcttcatgct gccgcagacc
     4801 ccgtcgatcg ggtcgtcgca gacgagccag tcttcgatcg tcgccgggtc gcggcgcagc
     4861 ttgagataga ccgtctggcc cgacatcaga tgacccgatc c
//
LOCUS       DASPGN010000119         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1191875, whole genome shotgun sequence.
ACCESSION   DASPGN010000119 DASPGN010000000
VERSION     DASPGN010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191875"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="VD788_03305"
     CDS             <1..1313
                     /locus_tag="VD788_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXV75320.1"
                     /translation="APYAERSASARRRLSVADEGREFDYRTQFQRDRDRIVYSRAFRR
                     LRQKAQTGILPAYEDHRRNRLTHTIEVCQLARTVGRALALNEDLIEAIALGHDLGQPA
                     FGAAGEQALDDLLSGRLDGRGGPGLGDLGGFSRVWQSLRVVDLIEKRYVHPGLNLTDA
                     VREGIFKSGPPAATRGEAIEGVRQGLPPRFESQVVILADRIASALHDLDDALQSGAVA
                     LNRVERLHAVVRLRAKLGQRYRPASGRFMRANAIHRGLIHLMVTGAIVASERVLSRWA
                     AKHGIDGPARFAALADDAVTGGEIDLPPAARRMLAEVESFLESTVRSGQAADRVDGRG
                     RRVITGLFAAYHADPRLLEDHLLLRFKEIAHRPFLRDLPRAEGEIEIDRRYRSDARFA
                     RLLVDHLAAMTDSFALAEHRRLMEMGAVPIPGAEQLRRERRVDG"
     gene            1394..2662
                     /gene="glyA"
                     /locus_tag="VD788_03310"
     CDS             1394..2662
                     /gene="glyA"
                     /locus_tag="VD788_03310"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXV75321.1"
                     /translation="MSFYRNLRAIDPEVAAALDGEVRRQAEGLELIASENFVSEAVLE
                     AMGSVLTNKYAEGYPGRRYYGGCEFADDVERLARERAKTLFGAEAANVQPHSGSQANQ
                     GVYFTVLEPGDAVLGMDLAHGGHLTHGHPLNLSGRLFRFFSYGVRRDDETIDYEALRE
                     SAHRHRPKLIVCGASAYPRTIDFPRICEVASEVEAVVMADIAHIAGPVATGRHPSPVP
                     CCDFVTSTTHKTLRGPRGGLILSKKKWAKDLNRTVFPGIQGGPFMHIIAAKATAFLEA
                     QQPSFADYVDRVLANAKRLASRVGQHGFRIVSGGTDNHLFLVDVFSRGITGKDAEIAL
                     EKAGITVNKNTIPFDRNPPMVASGLRIGTPALTTRGMGEAEMDRVGDLIASALEARDD
                     DPRLVAIKRDVRELAAGFPLYGERLAAARS"
     gene            2755..>3995
                     /locus_tag="VD788_03315"
     CDS             2755..>3995
                     /locus_tag="VD788_03315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HXV75322.1"
                     /translation="MAERVERVFARGGTLLIEAGTGTGKTLAYLVPAIAAGDRVVIST
                     GTRNLQDQILEKEIPFLRETLGVEFTACVMKGRENYLCRYRLGQFEREPLLEERDEAR
                     WVPRIAEWARRTGTGDRAEIVDLPDRLRMWRDVNARADTCSGSRCPEFDPCWLTQLKR
                     RAQQSQLVVVNHHLFFADLALRSAFGSVIPDYRLVVFDEAHQLEQVATLYFGVQLSSA
                     QIDELARDVERLATRGGGPPGGGGAAELRRASHELFGPLRDRVGAAAGRVRFDPVERG
                     GPDVEVEWAALCEAFDEVCRRLPGAAGDADETSGESIARRVDELRDALTRVLDRRDPG
                     FVYGMEIRGRGSLVLNASPIDVSQALRERLFASLDAAVLTSATLAVGGSFDFFARRLG
                     IDDAENDIVESPFDHRNQALL"
BASE COUNT          631 a         1332 c         1399 g          633 t
ORIGIN      
        1 tggcacccta cgccgagcgg tcggccagcg cgcgacgccg gttgtcggtc gccgacgagg
       61 ggcgggagtt cgactaccgc acccagttcc agcgggatcg cgatcgcatc gtctacagcc
      121 gcgcgttccg tcgcttgaga cagaaggcgc agaccgggat cctgccggcc tacgaggacc
      181 accgacgcaa ccggctgacc cacacgatcg aggtctgcca gctcgcacgc acggtcgggc
      241 gcgcgctggc gctcaacgaa gatctgatcg aggccatcgc actcggtcac gatctcgggc
      301 agccggcttt cggtgcggcg ggcgagcagg cgctcgacga cctgttgagc ggccggctcg
      361 acggccgcgg cggaccgggt ctcggcgacc tcggcggctt ctcgcgggtg tggcagagcc
      421 tgcgggtcgt cgatctgatc gaaaaacgct atgtccatcc gggactgaat ctgaccgacg
      481 cggtccgtga agggatcttc aagagcggtc cgcccgccgc cacgcgcggc gaggcgatcg
      541 agggtgtgcg tcaggggctg ccgccgcggt tcgaatcgca ggtcgtgatt ctggccgacc
      601 ggatcgcgag cgcgctccac gacctggatg acgcgctcca gtcgggagca gtcgcgctga
      661 accgcgtcga gcggcttcac gccgtcgttc ggctgcgcgc gaagctgggg cagcgctacc
      721 gccccgccag cgggcggttc atgagagcca acgcgatcca ccgcgggctg atccacttga
      781 tggtcaccgg ggcgatcgtc gcctccgagc gtgtgctgag ccgctgggcg gcaaagcacg
      841 gcatcgacgg gccggcacga ttcgcggcgc tcgccgacga tgcggtgacc ggcggcgaga
      901 tcgacttgcc gccggcggcg cggcgcatgt tggccgaggt cgaatcgttc ctcgagtcga
      961 cggtgcgttc gggccaggcc gccgaccgcg tcgatggccg cggtcggcgc gtgatcacgg
     1021 gcttgttcgc cgcctaccac gccgacccgc gtctgctcga ggatcacctg ctgctacggt
     1081 tcaaggagat cgcacaccgg ccgttcctcc gcgaccttcc ccgcgccgag ggcgagatcg
     1141 agatcgatcg gcgttatcgt tcggacgcac gattcgcccg cctgctggtc gatcacctcg
     1201 cggcgatgac cgacagcttc gccctggccg agcaccgtcg cctgatggag atgggtgccg
     1261 tgccgattcc cggcgcagaa caactgaggc gcgaacgtcg ggtcgatggc tgacggaccg
     1321 tgcttgccag tcctcggccg atcgactacg gtacgtgcgg acgcctcccc ccgccgccgc
     1381 cgaggaacag ccgatgagct tctaccggaa tctccgagcg atcgatcccg aagtggctgc
     1441 cgcactcgac ggcgaggtgc gtcgccaagc cgagggactc gagctgatcg cgtcggagaa
     1501 cttcgtcagt gaagcggtgc tcgaggcgat ggggtcggta ctgaccaaca agtacgccga
     1561 gggctacccc ggacgccgct actacggtgg ctgtgagttc gccgatgacg tcgagcggtt
     1621 ggcccgcgag cgggccaaga cgctgttcgg cgccgaggcg gccaacgtcc agcctcactc
     1681 gggcagccaa gccaaccagg gcgtctattt cacggtgctc gagccgggcg atgcggtgct
     1741 cggaatggac ctggcccacg gcggccacct gacccacggt cacccgctca acctgtcggg
     1801 ccgcctgttc cgcttcttct cctacggcgt acgacgcgac gacgagacaa tcgactacga
     1861 ggcgttgcgc gagtcggccc accggcatcg gccgaagctg atcgtctgcg gggcctcggc
     1921 ctatccacgc acgatcgatt tcccacggat ctgcgaggta gcatccgagg tcgaggccgt
     1981 cgtgatggcg gacatcgcgc acatcgcggg tccggtcgcg accggacgtc acccgagtcc
     2041 ggtgccgtgt tgcgacttcg taacgagcac cacgcacaag acgttgcgcg gcccacgggg
     2101 cgggctgatc ctgtcgaaga agaagtgggc gaaggacctc aatcgtaccg tctttcccgg
     2161 tatccaaggc gggccgttca tgcacatcat cgccgccaag gcgactgcgt tcctcgaggc
     2221 acagcaaccg tcgttcgcgg actacgtcga ccgggtgctg gccaacgcca agcggctcgc
     2281 atcccgcgtc ggccagcacg gcttccgtat cgtctccggc ggtaccgaca accacctgtt
     2341 tctggtcgac gtgttctcgc ggggcatcac cgggaaggac gccgagatcg cgctcgagaa
     2401 ggccgggatc accgtcaaca agaacacgat cccgttcgat cggaatccgc cgatggtcgc
     2461 cagcgggttg cgtatcggca ctccggcgtt gaccacgcgc ggaatgggtg aggccgagat
     2521 ggaccgggtc ggcgatctga tcgcgagcgc gctcgaggcg cgcgacgacg acccgcgtct
     2581 cgtcgcgatc aagcgcgacg tgcgcgagct agcagccggc ttcccgctgt acggggaacg
     2641 gctcgccgcg gcacgatcct gacgccggtg gaatcggtcg gcgcgttctt cgctcccggc
     2701 ggtcgccttt ctgccacaca cccgcggttc gagtaccggc cgggacagca gcgcatggcc
     2761 gaacgggtcg aacgcgtctt cgcccgaggt gggacgctgt tgatcgaagc cggtaccgga
     2821 acggggaaga ccctcgccta tctcgtgccc gcgatcgccg ccggtgaccg tgtggtgatc
     2881 tcgaccggca cgcgtaacct gcaagatcag atcctggaga aggaaattcc cttcctgcgg
     2941 gagacgctcg gggtcgagtt cacggcctgc gtgatgaaag gccgggagaa ctacctttgc
     3001 cgctaccgcc tggggcaatt cgagcgcgag ccgctgctcg aggagcgcga cgaggcgcgt
     3061 tgggtgccgc gcatcgccga gtgggcacgc cggaccggca ccggcgatcg cgccgagatc
     3121 gtcgatctac ccgaccgcct gcgcatgtgg cgcgacgtca acgcgcgggc ggatacctgc
     3181 agcggtagtc gctgcccgga gttcgacccg tgctggctga cacaactgaa gcgacgggcg
     3241 cagcagagtc agctggtggt ggtcaaccat cacctgttct tcgccgacct ggcgctccgc
     3301 tcggcattcg gttcggtgat acccgactac cgactggtcg tcttcgacga agcgcaccag
     3361 ctcgaacagg tggcgacgct ctacttcggg gtccagctgt cgtcggcgca gatcgacgag
     3421 ctggcgcggg acgtcgagcg gctggccacc cgcggagggg gaccgccggg cggaggcgga
     3481 gcggccgagc ttcgccgcgc gagccacgag ctgttcggcc cattgcgcga ccgggtcgga
     3541 gcggccgcgg gtcgggtgcg gttcgatccg gtcgaacgcg gagggcccga cgtcgaggtc
     3601 gagtgggcgg cactgtgcga ggcgttcgac gaggtgtgcc ggcggcttcc gggagcagcg
     3661 ggcgacgccg acgagacgag cggagaatcg atcgcccgtc gcgtcgacga gctgcgcgac
     3721 gcactgaccc gtgtgctcga tcgccgcgac cccgggttcg tttacgggat ggagattcgc
     3781 gggcgcggct cgttggtgct gaacgcctct ccgatcgacg tgtcgcaggc gctgcgggag
     3841 cggctgttcg catcgctcga cgcggcggtc ctcacctcgg cgacgctggc ggtcggcggc
     3901 agtttcgatt tcttcgcccg acgactcggt atcgacgacg ccgagaacga catcgtcgaa
     3961 tctccgttcg accatcgaaa tcaggcgttg ctcta
//
LOCUS       DASPGN010000120        10560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_723715, whole genome shotgun sequence.
ACCESSION   DASPGN010000120 DASPGN010000000
VERSION     DASPGN010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723715"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VD788_03320"
     CDS             complement(<1..917)
                     /locus_tag="VD788_03320"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HXV75323.1"
                     /translation="MTLPARTLEIRTSPHLLSGYSVDTIMFNVVLALVPTTLFAIYAF
                     GLTALLTLVTALGSCLATEWALCRAAARRTTLGDWSVTITGLLYGLTLPPGLPLWMVA
                     AGGLISVGLGKALFGGLGQNPFNPALVGRAVLQAAFPVAMTSWIPAFVTGRFSTLPGS
                     TLTLPFSTPRYDGVTTATPLQAWKFDRELAETADLALGYVSGSVGETSSLLILLGGLY
                     LIARKMMNWRIPVAILATVAAIAGLLHAVAPDRYASSPFMLFSGGLMLGAIFMATDMV
                     GSPMTGSGCVIYGALIGVLVVVIRVWGGMP"
     gene            complement(914..2251)
                     /gene="rsxC"
                     /locus_tag="VD788_03325"
     CDS             complement(914..2251)
                     /gene="rsxC"
                     /locus_tag="VD788_03325"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HXV75324.1"
                     /translation="MHLLGLRTFRHGVHPPEAKDETSGLAIRQFPFAPVLVVPLTQHA
                     GRPAIAEVREGQEVVRGQRIARPDGFLSVSMHAPASGIVRRVGLVPGIDGRMSPGIYI
                     VPHRASTQEIAGGTPCPLDGATPAEIVAAIQQAGIVGLGGAAFPTHVKLRIPEGKSVD
                     TLIINGVECEPYLTTDHRVMLEQSADLFIGLRYLLRVTGAERAIIGVEANKRDAADRL
                     RETLPADLPATVEVLRVKYPQGAEKMLITALLGREVPSGGLPLDVHALCINVATAAEI
                     GRLLPLGRGIQERVITVTGPAVKRKGNYRIPIGTPLRFLLDQVGVDADVDRVFLGGPM
                     MGQAASSLDIPITKGTSGVVAYTGGEAERTHRQIYPCIRCAYCIDACPIFLDPSQLGR
                     LAEHGEYDRMKESFHLIDCFECGSCSFVCPSHIPLVHRFRVAKAAVGRTKSQA"
     gene            complement(2267..7219)
                     /locus_tag="VD788_03330"
     CDS             complement(2267..7219)
                     /locus_tag="VD788_03330"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXV75325.1"
                     /translation="MSVLTLGREREKDGRHERERFKYPGVRAAMDGNTAVIACERESS
                     DAAGAYPITPSTQMGEYWAEAAASGHINISGRPLIFIEPEGEHAAAAVTAGMAMTGLR
                     AANFSSGQGIAYMHESLYAAVGKRLTYVLNIGARAMTKATLNVHAGHDDYHCIDDTGF
                     FQLFAKNAQSAADLNLIAHRLAELSLTPGAIAQDGFLTTHLIESMLVPERELIVEYLG
                     RPDDMIDAPTPAQRIIYGDKRRRIPLLWDVDNPVMAGLVQNQDSYMQSVAAQRPFFFD
                     HVRELADRAFDEFYRLTGRRYARVLGYRVDDAEYLLVGQGSLIPSAEAVVDYLRETRR
                     LKVGVVDLVMFRPFPADLVGRLLRGKKGVAVLERLDQPLAVDLPLVREIRAALSRCLE
                     NGRRPAQPFYPDLPTYRDVTDAPPLFSGSFGLGSRDLQPEGIVGAVENMLPDGAGRRF
                     FYLSIDFLREHPITPKQEIYQQTIESSYPQVKQLAVRGSENPNLMPPGSITVRFHSVG
                     GWGAITTGKNLAMTLFDLLGYHIKANPKYGSEKKGQPTTYYLAAAPEPIRVNCEYFYV
                     DVVLSPDPNVFHHTNALAGLKQGGVFVLQSSQPTAEAVWQSIPPAFQRIIIDKNIRVF
                     YLDAFRIAREEASDPDLQLRMQGIAFQGAFFAASPLKQQASLDDAQLLRAIREQLEHK
                     FGSKGARVVEDNMRVVKRGFDEVQPVPVGAVSEPSATGRTAGSEPPIPEMVKRLPASE
                     APMSDIHRFWEQTGNFYARGMGNDNLTDPFIGLGVMPACTALFRDMTQIRFEHPAWNA
                     EACTACGKCYTVCPDTAIPGLVNTVDQVFDTAISRLRKRGTQLQQLPRAARLAEQALR
                     RSLNEAQPTDSVAEMMEQALQQVAGRDGLSADERKRLVAEIDLLRDELDGFQFALSRP
                     YYSIPEQQQPGKGALLSITVNPYTCKGCMECVEVCDDDALRPVTQSDDSVARLRAHWD
                     FWLDLPNTPREYIRVDDIDQGIGALETILLDKRNYLAFTSGEGACVGCGEKTVIHLFV
                     ATVEALMQPRVERHLARLDELIGKLERHVRLELVSEISVGDRMGKIVDDYADRDLTLA
                     EIASRMERDGDGKPIDQEWLTRVTDLVSRLRELRWRYVEGTSGGGRSRMGMINATGCT
                     SVWGSTYPFNPYPFPWANHLFQDSASMAMGVFEGHMVKMADGFKAIRMAELELDGKYD
                     PTQHDEFFTYFNWQQFTDDEWDLCPPVVAVGGDGAMYDIGFQNLSRAMASGKPIKLLV
                     LDTQVYSNTGGQACTSGFVGQISDMAQFGKVIQGKQEPRKEIALIGIAHRTTYVLQST
                     IAHPGHMIEGFIRGLRARRPALFNLYASCQPEHGIGDDMSAEQNRLAVESRAYPLFRY
                     DPDAGKTVEECLDLDGNPALDQDWPTYTMRYRDGGREREMTVPMTFADFAVTEVRFRK
                     HFRHAPPDTWNDSMVPLAEFLGLTADEREGRFPYLWSVDRNHRLTRLLVDRTLVASCE
                     ERRDFWTMLRALAGVGRVEIPREEIEADVRRELVGQIASGLLRLAGGSAEPADVASAV
                     AAVAAGPAPAAGTTGDYLAPWIDSAECTSCDECIKLNGKIFAYDGQKKAFIKDPQGGP
                     YRDLVKAAERCTARVIHPGLPRDRSAKEIDKWIARAEKYN"
     gene            complement(7254..8117)
                     /locus_tag="VD788_03335"
     CDS             complement(7254..8117)
                     /locus_tag="VD788_03335"
                     /inference="COORDINATES: protein motif:HMM:NF015987.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HXV75326.1"
                     /translation="MLFLTAVGTIGGLTFALASFLVLANRKLHVEEDPRIEIVEDLLP
                     HVNCGACGFPGCRPFAEALVAGRALPGKCTVSSEPGRQSIASFLGVDVGFEERRVARL
                     ACAGGTNVARNRARYDGIASCAAAARVAGGGKSCFWGCLGLADCRDACTFDAIRMDRH
                     ALPVVDEDKCTACGDCVTACPKDLFSLQPISRRLWVACRSLDAGDELLEDCAVACTAC
                     GRCASDAPGLIRMHDNLPVIDYDRPHQTTVPIQRCPTGAIVWIDPDRGPTKGPAAHKI
                     VRQGALRDAKT"
     gene            complement(8165..9202)
                     /locus_tag="VD788_03340"
     CDS             complement(8165..9202)
                     /locus_tag="VD788_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXV75327.1"
                     /translation="MSDRDAAKSIVPLGRRDLLRILAVGGGLGAAWTVGWVNRKRAVP
                     VSRSAVLMGTRVNLTVMGDDRDAADAAARVTLGRMAELESLLSRHRPDSELSRLNAAG
                     RIDGASDALIDLLGLAHDVSAAGAGAFDVSMLPLLDVYRDPRRRWGELPAPDEIEHAL
                     ERVDYRQIRVDTTTRRVSLLRDGMRLTLDGIAKGYIVDRGVDVLRERGFANVFVEAGG
                     DLMASGDKGGLPWRIGIRDPRASGLTLCARFDTTNRAVATSGDYMQPFTRDLTQHHIL
                     DPRTGYSSPELASSTVIARDAATADALATLTMVLGPRRGRELLESLAGCEGYFVAKDL
                     EVTRTSGFSVA"
     gene            complement(9318..>10560)
                     /locus_tag="VD788_03345"
     CDS             complement(9318..>10560)
                     /locus_tag="VD788_03345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV75328.1"
                     /translation="YGERLGEPDELGPLYELLGDVLRRRFGGWRAWVLSGNRALDKRL
                     GLRPASRTVLYNGPIECRLLELPIAGAPPRRPGGPAWRKTDPQVSGFAKRLRKNLREC
                     ERWAVRSGTDCYRLYDADVPTFNLAVDRYGGAARVEEYERPAGVDPVAADRRLRSALD
                     AVADVLGIARAEVVLRVRRRLAPGEQHGRYGDRGRLREVRERGLRFLVNLEDYLDTGL
                     FLDDRMLRAWLAERARGSSFLNLFAYTCAATVAAARGGARESVSVDLSRRYLDWGRRN
                     LELNGLDPTRHRTVRADVWSRLGGGSRSRRFDLVFVAPPSRSRSKAMPHEFDLRRDHV
                     EMLARLGGWLARGGTVVFASHQRRFELDVAALPQFTSREITHEVTPPDFARRPRLRAW
                     ALEVRQTDPAADDAPVGRLRE"
BASE COUNT         1679 a         3625 c         3624 g         1632 t
ORIGIN      
        1 ggcatcccgc cccacacgcg gatcacgacg accaacacgc cgatcagcgc gccgtagatc
       61 acgcatccgc tcccggtcat cggcgagccg accatgtcgg tcgccatgaa gatcgcgccg
      121 agcatcagcc cgccggagaa gagcatgaac ggcgaggagg cgtagcggtc gggggcgacg
      181 gcgtgaagca agccggcgat cgccgctacg gtggcgagga tcgcgacggg gatccgccag
      241 ttcatcatct tgcgcgcgat caggtagagc ccgccgagca ggatcaacag gctgctcgtc
      301 tcgccgaccg agccgctgac gtagcccagc gcgagatcgg cggtctcggc cagctcgcgg
      361 tcgaacttcc aggcctgcag gggtgtcgcc gtcgtcaccc cgtcgtaccg cggcgtgctg
      421 aacggcagcg tcagcgtcga gccgggcagc gtcgagaacc ggccggtgac gaacgccggg
      481 atccaactgg tcatcgcgac cgggaacgcc gcttgcaaca ccgcccgtcc caccagcgcc
      541 gggttgaacg gattctgtcc gaggccgccg aacagggcct taccgagtcc gacggagatc
      601 aggccgccgg cggcgaccat ccacaacggc aggccggggg gcagcgtcag accgtagagc
      661 agaccggtga tcgtcaccga ccagtcgccc agcgtcgtgc ggcgagcggc ggcccggcac
      721 aacgcccact ccgtcgccag acacgagccg agcgccgtca cgagcgtcag caacgccgtc
      781 agcccgaaag cgtagatcgc gaacaacgtg gtcggtacga gcgccagcac gacgttgaac
      841 atgatcgtgt cgacgctgta gccgctgagc aggtggggcg aggtacggat ctcgagcgtg
      901 cgtgccggta gcgtcatgcc tggctcttgg tccggccaac cgcggctttg gccacgcgga
      961 accgatgaac cagcggaatg tgcgacgggc agacgaacga gcacgagccg cactcgaaac
     1021 agtcgatcag atgaaagctc tccttcatcc ggtcgtactc gccgtgctcg gccagacgac
     1081 cgagctgact gggatcgagg aagatcggac acgcgtcgat gcagtaggcg cagcggatgc
     1141 aggggtagat ctgtcggtgg gtccgctcgg cctcgccacc ggtgtaggcc accacgcccg
     1201 acgttccctt ggtgatcggg atgtcgagcg acgaggcggc ctgtcccatc atcggcccgc
     1261 cgagaaagac ccgatcgacg tcggcgtcga cgccgacctg atcgagcagg aagcgcaacg
     1321 gggtgccgat cgggatccgg tagtttccct tgcgcttgac ggccggcccg gtgacggtga
     1381 tgacccgttc ctggatcccg cggccgagcg gcagcagtcg gccgatctcc gccgcggtgg
     1441 cgacgttgat gcacagcgcg tggacgtcga gcggcaatcc acccgacggc acctcgcggc
     1501 cgagtagcgc cgtgatcagc atcttctcgg cgccctgcgg gtacttgacg cgcagcactt
     1561 cgacggtcgc cggcagatcg gcgggtagcg tctcgcgcag ccgatcggcg gcgtcgcgtt
     1621 tgttggcctc gactccgatg atcgcccgct cggcgccggt cacccgcagc aggtagcgca
     1681 ggccgatgaa cagatcggcg ctctgctcga gcattacacg gtgatcggtc gtcaggtacg
     1741 gctcgcactc gaccccgttg atgatcagcg tgtcgaccga tttgccttcg gggatcctca
     1801 gcttgacgtg tgtcggaaac gccgcgccgc ccaacccgac gatcccggcc tgctggatcg
     1861 ccgcgacgat ctcggccggc gtcgcgccgt cgagcgggca cggcgtcccg ccggcgatct
     1921 cctgtgtcga tgcccggtgc ggcacgatgt agattccggg gctcatgcgg ccgtcgatgc
     1981 ccggaaccag gccgacgcgc cggacgatcc cggaggcggg cgcatgcatc gacaccgaca
     2041 ggaagccgtc cggacgcgcg atgcgctgac cgcggacgac ctcctgaccc tcgcggacct
     2101 cggcgatcgc cgggcgtccc gcgtgctggg tcagcggcac gaccagcacc ggcgcgaacg
     2161 gaaactggcg gatcgccagc ccgctcgttt cgtccttcgc ctccggcggg tggacgccgt
     2221 ggcggaacgt cctcagcccg agcaagtgca ctcgttcacc cgcggttcag ttgtacttct
     2281 cggcccgtgc gatccacttg tcgatctctt tcgccgaccg atcacgcggt agaccgggat
     2341 ggatcacccg cgccgtgcat cgctcggccg ccttgaccag gtcacggtac ggcccgccct
     2401 ggggatcttt gatgaacgcc tttttctgac cgtcataggc gaagatcttg ccgttgagct
     2461 tgatgcactc gtcgcacgac gtgcactcgg cgctgtcgat ccagggcgcg aggtagtcgc
     2521 cggtcgtacc ggcagccggc gcggggcccg cggcgacggc ggcgaccgcc gaggcgacgt
     2581 ccgccggttc ggcgctccca cccgccagac gcagcagccc gctcgcgatc tgacccacca
     2641 gttcccgtcg cacgtcggct tcgatctcct cgcgcgggat ctccacacgt ccgacgccgg
     2701 cgagcgcgcg cagcatcgtc cagaagtcgc gtcgctcttc gcacgaagcg accagcgtgc
     2761 ggtcgaccag tagccgcgtc agccgatggt tgcggtcgac cgaccacagg taggggaatc
     2821 gcccctcgcg ctcgtcggcg gtcagcccga ggaactcggc cagcggcacc atcgagtcgt
     2881 tccaggtgtc cggcggggcg tggcggaagt gcttgcggaa ccgcacctcg gtcaccgcga
     2941 aatcggcgaa ggtcatcggc acggtcatct cgcgctcgcg gccgccgtcg cggtaacgca
     3001 tggtgtaagt cggccagtcc tgatcgagag ccgggtttcc gtcgaggtcg agacactcct
     3061 cgaccgtctt tccggcgtcg ggatcgtagc ggaacagcgg ataggcgcgc gactcgacgg
     3121 ccagccggtt ctgctccgcg ctcatgtcgt cgccgatgcc gtgctccggc tggcacgagg
     3181 catagaggtt gaacaacgcc ggccggcgcg cccgcagccc gcggatgaac ccctcgatca
     3241 tgtgaccggg atgggcgatc gtgctttgca gcacgtaggt cgtgcggtgg gcgatgccga
     3301 tcagcgcgat ctccttgcgc ggctcctgct tgccctggat caccttgccg aactgagcca
     3361 tgtcggaaat ctggccgaca aagccggaag tgcaggcctg accgccggtg ttggagtaga
     3421 cctgggtgtc gagcaccagc agcttgatcg gcttgccgga ggccatcgcg cgcgacagat
     3481 tctggaagcc gatgtcgtac atcgcaccgt cgccgccgac cgcgacgacc ggcgggcaga
     3541 gatcccactc gtcgtcggtg aactgctgcc agttgaagta ggtgaagaac tcgtcgtgct
     3601 gcgtcgggtc gtacttgccg tcgagctcga gctcggccat gcggatcgcc ttgaagccgt
     3661 cggccatctt gaccatgtgg ccttcgaaca cacccatcgc catcgacgcc gaatcctgga
     3721 acaggtggtt ggcccacgga aacggatagg ggttgaacgg gtaggtgctg ccccaaaccg
     3781 acgtgcaccc ggtggcgttg atcatcccca tgcgcgaacg gccgccgccg ctggtgccct
     3841 cgacgtagcg ccaacgcagc tcgcgcagcc gactgaccag gtcggtcacc cgcgtcagcc
     3901 actcctggtc gatcggtttc ccgtcaccgt cgcgttccat tcgcgaggcg atctcggcca
     3961 gcgtcaggtc gcgatcggcg tagtcgtcga cgatcttgcc catgcgatcg ccgacgctga
     4021 tctccgacac cagctcgagc cggacgtggc gctcgagctt gccgatcagc tcgtcgaggc
     4081 gcgccaggtg ccgttcgacg cgcggctgca tcaacgcttc gacggtggcg acgaacaggt
     4141 gaatcaccgt cttctcgccg cagccgacgc atgccccctc gccgctggtg aacgcgaggt
     4201 agttgcgctt gtcgagcagg atcgtctcga gcgcaccgat tccctggtcg atatcatcga
     4261 cgcggatata ctcgcgcggc gtgttcggta gatcgagcca gaagtcccag tgggcgcgca
     4321 atcgggcgac cgagtcgtcg ctctgggtca ccggtcgcag cgcgtcgtcg tcgcagacct
     4381 cgacgcactc catgcagccc ttgcaggtgt aggggttgac ggtgatcgac agcagcgctc
     4441 ccttgccggg ctgctgctgt tcggggatcg agtagtacgg acggctcagc gcgaactgga
     4501 agccgtcgag ctcgtcgcgc agcaggtcga tctcggccac caggcgcttg cgctcgtcgg
     4561 ccgacagccc gtctcgaccg gcgacctgct gcagcgcctg ctccatcatc tcggcgacgg
     4621 aatcggtcgg ttgcgcctcg ttgagcgagc gcctcaacgc ctgctcggcg agccgcgcgg
     4681 cacgcggcag ctgctgcagc tgggtgccgc gcttgcgcag ccgggagatc gcggtatcga
     4741 acacctggtc gacggtgttg accaagccgg ggatcgcggt atccgggcag accgtgtagc
     4801 acttgccgca ggcggtgcag gcctcggcgt tccatgccgg atgctcgaag cggatctgcg
     4861 tcatgtcgcg aaacagggcg gtgcaggccg gcatcacgcc cagcccgatg aacggatcgg
     4921 tcaggttgtc gttgcccatc ccacgggcgt agaagttgcc ggtctgctcc cagaaccggt
     4981 ggatgtcgct catcggcgcc tcgctcgccg ggagccgctt gaccatctca gggatcggcg
     5041 gctcgctgcc cgccgtgcgt ccggtggcgg acggttcgct gacggcgccg accggaaccg
     5101 gttggacctc gtcgaagccg cgcttgacga cccgcatgtt gtcctcgacc acgcgggcac
     5161 ccttcgagcc gaacttgtgc tcgagctgct cgcggatcgc gcgcagcagc tgggcgtcgt
     5221 cgagcgaggc ctgctgtttc agcggcgagg cggcgaagaa cgccccctga aacgcaattc
     5281 cctgcatcct cagttgcagg tcggggtcgc tcgcctcctc gcgcgcgata cgaaacgcgt
     5341 cgagatagaa cacgcggatg ttcttgtcga tgatgatccg ctggaacgcc ggcgggatcg
     5401 actgccagac cgcctcggcg gtcggttgcg agctctgcag cacgaacacg ccgccctgct
     5461 tcaggccggc caaggcgttc gtatggtgga acacgttggg gtcgggcgag aggacgacgt
     5521 cgacatagaa gtactcgcag ttgacgcgga tcggctccgg cgccgcggcg agatagtagg
     5581 tggtgggctg tcccttcttc tccgagccgt acttcgggtt ggccttgatg tggtagccca
     5641 acaggtcgaa cagcgtcatc gccaggttct tccccgtcgt gatcgcgccc cagcctccga
     5701 ccgagtggaa ccgcacggtg atgctgcccg gcggcatcaa gttggggttc tccgagccgc
     5761 ggacggcgag ctgcttgacc tgcgggtagg agctttcgat cgtctgctgg tagatctcct
     5821 gcttcggcgt gatcgggtgc tcgcgcagga aatcgatcga caggtagaag aagcgccggc
     5881 cggcgccgtc gggcagcatg ttctcgaccg cgccgacgat gccctcgggt tggagatcgc
     5941 ggctacccag gccgaacgac ccggaaaaga gcgggggcgc gtcggtgacg tcgcgatagg
     6001 tcggcaggtc cgggtagaac ggctgcgccg gccggcgccc gttctcgaga cagcgcgaca
     6061 gcgcggcgcg gatctcgcgt acgagcggca gatcgacggc cagcggctgg tcgaggcgtt
     6121 cgagcaccgc gacccctttc ttgccgcgga gcagacgtcc gaccaggtcg gccggaaacg
     6181 gccggaacat caccaggtcg acgacgccga ccttcaggcg ccgcgtttcg cgcaagtagt
     6241 cgacgaccgc ctccgcgctc gggatcaggc tgccttgacc gacgagcagg tactcggcgt
     6301 cgtccacgcg gtaacccagc acgcgggcgt agcgccggcc ggtcagtcga tagaactcgt
     6361 cgaatgcacg atcggccagt tcgcgcacgt ggtcgaagaa gaacgggcgt tgggcggcga
     6421 cgctctgcat gtaggagtcc tggttctgca ccagtccggc catcaccgga ttgtcgacgt
     6481 cccacagcaa cggaatccga cgccgcttgt cgccgtagat gatgcgctgc gccggggtcg
     6541 gcgcgtcgat catgtcgtcg ggacggccaa gatactcgac gatcagttct cgctcgggca
     6601 ccagcatcga ctcgatcaga tgggtggtca agaagccgtc ctgggcgatc gcgcccgggg
     6661 tcaacgacag ctcggcgagg cgatgcgcga tcagattcag gtcggccgcg ctctgggcgt
     6721 tcttggcgaa cagttggaaa aagccggtgt cgtcgatgca gtggtagtcg tcgtgaccgg
     6781 cgtgcacgtt cagcgtggcc ttggtcatcg cccgcgcgcc gatgttgagc acgtaggtca
     6841 gccgcttgcc gacggcggcg taaagcgact cgtgcatgta ggcgatgcct tggccggagg
     6901 agaagttggc ggcgcgcagc ccggtcatcg ccatgccggc cgtgaccgcg gcggctgcat
     6961 gctcgccctc gggctcgatg aagatcagcg gtcggcccga gatgttgatg tgcccgctcg
     7021 ccgcggcctc cgcccagtac tcgcccatct gcgtcgacgg tgtgatcggg tacgcgcctg
     7081 ccgcgtcgct cgactcgcgc tcgcaggcga tcaccgcggt gttgccgtcc atcgccgcgc
     7141 gcaccccggg atacttgaag cgctcccgct cgtgccggcc gtctttctcg cgttctcgcc
     7201 ccagcgtcaa tacggacatc gcggctccct aggcgttccg ctgcggaacg gtgtcaagtc
     7261 ttggcatccc ggagggctcc ctggcgaacg atcttgtgcg ccgccggacc cttggtcggg
     7321 ccgcggtcgg ggtcgatcca gacgatggct ccggtcggac agcgctgaat cggtacggtc
     7381 gtctgatgcg gccggtcgta gtcgatgacc ggcaggttgt cgtgcattcg aatcagcccc
     7441 ggcgcgtccg acgcacagcg gccgcaggcg gtgcaggcga cggcgcaatc ctcgagcagc
     7501 tcgtcgccgg cgtcgagcga gcggcaggcg acccacagcc ggcggctgat cggctgcagc
     7561 gagaacaggt ccttcgggca cgccgtcacg cagtcgccgc acgccgtaca cttgtcctcg
     7621 tcgacgacgg gcagcgcgtg ccggtccatc ctgatcgcgt cgaacgtgca agcgtcgcgg
     7681 cagtcggcca ggccgagaca tccccagaag cagctcttgc cgccgccggc gacgcgcgcg
     7741 gccgcggcgc agctggcgat gccgtcgtag cgcgcccggt tgcgcgcgac gttggtgccg
     7801 ccggcgcagg ccagccgggc cacgcgacgc tcctcgaaac cgacgtcgac gccgaggaac
     7861 gaggcgatcg actggcgccc gggttcgctg ctgaccgtgc acttgcccgg caaggcccgg
     7921 ccggccacca gcgcctcggc gaacgggcgg caacccggga agccgcaggc gccgcagttg
     7981 acgtgcggca gcagatcctc gacgatctcg atgcgcgggt cttcctcgac gtggagcttc
     8041 cggttggcca gcacgagaaa cgacgccagg gcgaacgtca agccgccgat cgtaccgact
     8101 gcggtcagga acagcatccg tcgacctcgt cgattggccg cgccgagcga cgacgcgggc
     8161 cgtgctacgc gaccgagaag cccgacgtgc gggtgacctc caggtccttc gccacgaaat
     8221 agccctcgca cccggcaagc gattccagca gctcccgccc gcggcggggt ccgagaacca
     8281 tcgtcagcgt ggccagtgca tcggcggtcg ccgcgtcgcg cgcgatcacg gtgctgctgg
     8341 ccagctccgg cgaggaatag cccgtccgcg gatcgaggat gtggtgctgg gtcaggtcgc
     8401 gggtgaacgg ctgcatgtag tcgccggagg tggcaacggc ccggttcgtc gtgtcgaagc
     8461 gcgcgcacag cgtcagcccg gaagcgcgag gatcgcggat gccgatccgc cacggcaggc
     8521 cgcccttgtc gcccgaggcc atcagatctc caccggcctc gacgaagacg ttcgcgaagc
     8581 cacgttcgcg cagcacgtcg acgccacggt cgacgatgta gcccttggcg atgccgtcga
     8641 gcgtgaggcg catgccgtcg cggagcagac tcacccggcg cgtcgtggtg tcgacgcgga
     8701 tctggcgata gtcgacgcgc tcgagcgcgt gctcgatctc gtccggtgcc ggcagctcgc
     8761 cccagcgccg ccgcgggtcg cggtagacgt cgagcagcgg cagcatgctg acatcgaacg
     8821 cccccgcccc ggccgccgac acgtcatgtg ccaggccgag caggtcgatc agcgcgtccg
     8881 aagcgccgtc gatgcgtcct gcggcattca gccgcgacag ctcgctgtcg ggccgatgac
     8941 ggctcagcag gctctcgagc tccgccatcc gcccgagcgt cacgcgggcc gcggcatcgg
     9001 cggcgtcccg gtcgtcaccc atcaccgtca ggttgacccg agtccccatc agcaccgccg
     9061 aacggctgac cggcacggct cgcttgcggt tgacccagcc gacggtccat gcggcgccga
     9121 gccctccgcc gacggcgagg attctcagca ggtcgcggcg acccagcggc acgatcgatt
     9181 tggcggcgtc tcgatctgac acgatgctcc cgtccggacc gatcccggtc tcctggaccc
     9241 ggcccgagtc ccagagcggg cattctaccg acagcgtccg gcggccaaca ggggggggat
     9301 gagagtggag ccctccctta ttcacggagc cggccgaccg gcgcgtcatc cgccgcgggg
     9361 tccgtctggc ggacctcgag cgcccaggcc cgcagccgtg gccggcgtgc gaaatccggc
     9421 ggggtgacct cgtgcgtgat ctcgcgcgag gtgaactgcg gaagcgcggc gacgtcgagt
     9481 tcgaagcgcc gctgatgact ggcgaacacg accgtcccac cgcgagcgag ccagccaccg
     9541 aggcgggcca gcatctcgac gtgatcgcgt cgtaggtcga actcgtgcgg catcgccttc
     9601 gagcgcgaac gggacggtgg cgcgacgaag accagatcga agcgacggga gcgggatccc
     9661 ccgccgagac ggctccacac gtcggctcgc accgtccggt ggcgggtcgg gtcgagtccg
     9721 ttcagctcca agttgcgccg gccccagtcg aggtagcggc gcgacaaatc aacgctgacc
     9781 gattcgcgcg cgccgccgcg agcggcggcg acggtcgccg cgcaggtata ggcgaacagg
     9841 ttgagaaagg acgagccccg cgcgcgctcc gcgagccagg cccgcagcat ccggtcgtcg
     9901 aggaacagcc ccgtatcgag gtaatcctcg aggttcacga ggaaccgcaa cccgcgctcg
     9961 cgcacctcgc gcagacgtcc ccgatcgccg tagcgtccgt gctgctcccc cggtgcgaga
    10021 cgacggcgga cgcgcagcac gacctccgcc cgggcgatgc ccaacacgtc ggcgaccgcg
    10081 tcgagcgcgg agcgcagccg gcggtcggca gcgacgggat cgacaccggc cggtcgctcg
    10141 tactcctcga cgcgggccgc cccgccgtac cgatcgaccg ccaggttgaa ggtcggcacg
    10201 tcggcgtcgt acagccggta gcaatcggtg ccgctgcgca cagcccaccg ctcgcattcg
    10261 cgcagattct tgcgcaaccg tttggcgaag cccgaaacct gcggatccgt cttccgccac
    10321 gccggtccgc cgggccgacg cggcggggcg ccggcgatcg gcagctcgag caggcggcac
    10381 tcgatcggac cgttgtagag caccgtgcgc gacgccggtc gcagaccgag ccgcttgtcg
    10441 agcgcgcggt tcccgctcag cacccacgcg cgccagccgc caaaacggcg tctcagcacg
    10501 tcgcccagca gctcgtacag cggcccgagc tcgtcgggct cgccgagccg ctcgccgtag
//
LOCUS       DASPGN010000121        12553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1661025, whole genome shotgun sequence.
ACCESSION   DASPGN010000121 DASPGN010000000
VERSION     DASPGN010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12553
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661025"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2626
                     /locus_tag="VD788_03350"
     CDS             <1..2626
                     /locus_tag="VD788_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75329.1"
                     /translation="ADLLQGDNAPQPYDSSLDGRLAFYMNGRVSDRWKLTASADTREG
                     PVEDLFSNFLDKSPDSLFRRIDPDYHYPTFGDDSVVQQMAPTQGKLYVKASRGESYGL
                     WGNFKVGYLGNELAQVDRGLYGANAHYETEATTRFGERRIAVDGFAAEPGTIASYEEF
                     RGTGGSLYFLRRQDILTGSERVRIEVRDKASGIVTGVVNLRPNTDYDIDYLQGRLLLS
                     EPLASTPDDNLLVRTSGLSGDEAYLVVRYEYTPGFDELDAVAVGGQGHVWFTDHLRLG
                     LTGNSNEEGSADSSLGAADLTLRKSAESWVKLQAGRTDGLIQSALRSEDGGFEFQSTD
                     DPSFIDADADAYRADLSVGLGDFFAGPDGRLTLYAQTLDAGYSSPGQATIKETELYGG
                     TFRMPVTRRLSLAAKGDQRTEELGLESRALELNVGYRLTDEWSVSAGARNDRREDRSP
                     VIPLTQEEGERTDAVLQVGFDPGAAWRAYGFVQDTVESSGNRDDNGRIGAGGSYRLSK
                     RFKIDGEVSDGDLGPGGRVGTSFLYSERTHLYLNYALENERTDNGMRIRRGTLVSGVK
                     RRLSDSSSVYLEERYQDSGSLSGLTHATGIDLVAGERWNLGASAELGTLINSQTSAET
                     DRRAAGVRLGYGVETMQLSTAIEYRRDEQEQLDTTLDERKVWLFRNTFKFQLSPDSRV
                     VGKLNHSFSDSSLGEFYEGGYTEGVVGYAYRPVRYDRLSALAKYTYFYNMPTADQLGA
                     DSTPSEYVQKSHIAALDLSYDLSAAWSIGGKYAYRLGQVSLDREQPEFFDNAAQLVVF
                     RVDWQFLEGWESLAEVRALELPDLGQRRGGALFSIYRYIGKHLKVGAGYNFTDFSDDL
                     TDLSYDHQGVFVNLIGTM"
     gene            2717..3787
                     /locus_tag="VD788_03355"
     CDS             2717..3787
                     /locus_tag="VD788_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXV75330.1"
                     /translation="MGLRTRGTSSFVGALAVALLVSCRAPDGTVGPEDRSFVERPGEG
                     PNVVEIATPWLDGCGSGCGNAYLELARRFAERGAPISAAELDRRLAAAEATADAPLLG
                     LGRDELARRIVAELRIGWMLDDRERRGLAVAQLAERRGDGFVRRKLLFWDPLVGSFEG
                     TLLLPGGSGRHPAILGLHGHRRDDETFVRDYFGEELAARGFAVLVPRLRADDCSRSEN
                     RIARELLERGFTLMGLRVYEALRMVDYLDTLPQVDAGRIALFGHSGGSSLANLLLRVS
                     DRFDAAVVDHEVDFRKRRGPCHLHCETIPGMFPLAADINDRRTLAIPWMRASYKYVRE
                     GESGQVIDFLDRRLALVATTDP"
     gene            complement(5360..5683)
                     /locus_tag="VD788_03360"
                     /pseudo
     CDS             complement(5360..5683)
                     /locus_tag="VD788_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5994..7175
                     /locus_tag="VD788_03365"
     CDS             5994..7175
                     /locus_tag="VD788_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HXV75331.1"
                     /translation="MIRTHLSADPAESFGWAAARIGDLDGDGAPEYLISAIFNAQNGF
                     NAGRVVLYSGADGSVMNEVFGNPGDRLGHQLASAGDVNGDGVLDYALGGPGSVPAPGP
                     QPGRLLVLSGADHSVLLDVAGPGDRTFFGYDVHAAGDLNGDGFDDLVVGAPRDSTSFA
                     FAGRAYAISGRDGSTLWTYGGEQGFYLVGTGVAGIGDLNGDGIPEQVAAGFQAGHKFQ
                     GEGYVLSGADGTVLHVLKPKNEPRQNFGNFFVQNAGDTDRDGVDDILIGDFGIPSTPG
                     RAYLWSGADTEARQILDGANAADGFGIGRGVGDVDGDGHADLFLAAFHNSEGAFQAGK
                     GYVYSGRNLKRIREMTGTVAGAQLGFDALGLGDVNADGRIDFMLTGVGVAHVIAGNPV
                     P"
     gene            complement(7241..9694)
                     /locus_tag="VD788_03370"
     CDS             complement(7241..9694)
                     /locus_tag="VD788_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75332.1"
                     /translation="MAKERFAVFLAVLCLATGWVDAQVLLQLTDLDRETALTPALDDA
                     GEQVFANVNADAFGGNPGGVFQLGAWNAVTGIGSALVEDPDGVSWMRRSVAVSADGEW
                     LAFVSPADLTGQNHDEGLEIFVIQPDGSGLAQLTNHVGPANTSWIYRVAISGNGDRVA
                     FIADTDPLGANPDRVPQVFAVDRDGSSPIQLTAATSADQSPGSHWTGTHLDVDISDAG
                     TVVLFHHAPGLFVILDDGSGLQPVEGGIFSYLSGNGLHAASEMNSSAVVVDPWMGFIE
                     LSEQGSSPSITADGSFVYFSDNRIDPSDNPEGNDEIWQASLDGSIVAAVTSTTGRSNV
                     DPVVSGDGTRIAFYQRWTTDSLRAIDFRGDNEVVLAELTGDDPYVFAPEITPDGSRIV
                     YSLSGGGMLRVDPADGEHQLVVPFEVEAKSITADGETIVFQAYEDPLGIGNDLGNPQV
                     YAVEADGTGLRVLSGPFVPGQGAFFPVIAANGSWVVFVGLFDQELYRVRPDGSEFAKF
                     TNLAGSGNFYPRIDANGEWVVFENQGIARIRTDGSGFQQIVSAGLFETHWPDISGSGQ
                     QIVWAGSNDPLGTNPDGNSEIFRYDVQAATIDQVTVTTVGSSSRPRISKDGNWIYFNS
                     SVPFFDENPQRRHEPYRYEIASGTIERVGGIRRGPTDLACSLVDPDCNMAVDGTGRRA
                     VFTAAGDWAGANPDYGSELYLVDQQRGSRIEAGKSAPSVVTWDADPRYVRYDIVRGDL
                     ANVGYDDGTIALGPVTCLEQVSPDTDTRGFEDELQPAPGQVLVYVYRGTQGTGAAVGD
                     WGVGTGGFTRVPGTGSCGP"
     gene            complement(9776..10243)
                     /locus_tag="VD788_03375"
     CDS             complement(9776..10243)
                     /locus_tag="VD788_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXV75333.1"
                     /translation="MTPPELERAVMSNIASVLKQEITRLSRKATKSETAVLRRATAQY
                     RRDIAELKRQVAGLSKQVAYLERQERKRATSPAPAAVVEGKRFSRRGLETHRKKLDLS
                     AADYARLVGVTGQTVYNWEQGKSRPRDEQLAALVEIRGLGKREAQKRLELLEG"
     gene            complement(10298..11803)
                     /locus_tag="VD788_03380"
     CDS             complement(10298..11803)
                     /locus_tag="VD788_03380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV75334.1"
                     /translation="MIADGERSGPSTGRWWLLLAGATALHAAVAVVTPLSGDEAYYWD
                     LSRHPAWSTFDQPPLVLWSMVPFRLVLGETALAVRAPAIVASLAIALLMLPLARRLGG
                     GVGEAALAYLVLHATPLVLFGSSYVSTDIVMTACFVGAAWAAVAIAQGDRRGWWGFGL
                     AAGLGFLAKFPMVAVLIAVVRLLRMRHVRAQLRTLTPWLAGATSLMLTAPVWIWGARH
                     GWDNVTFQSGRVPDAARFSVRHVVEFVAANLALSSFVGVAFAVAWWRARARREPGWVA
                     VRWVAGASFVLFAAFALRGRVAAHWGAPTLVLSALLLVFHRFRGWKIWTLAGAATSVV
                     LLALVLAICADPAAFIARCRTAGGGPLAAIPADKLARMVGYDETAAVLRDVLRPGEIV
                     ASESYSMVHLVAFAAGGTLPVHLADVNRGVHGLAALYWFPPERFDGVDLVWVTGRRET
                     LVALADICREVHPLPPVVVRARGEPVREITMARCLEARPAPGVMSRLPPGR"
     gene            complement(11800..>12553)
                     /locus_tag="VD788_03385"
     CDS             complement(11800..>12553)
                     /locus_tag="VD788_03385"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV75335.1"
                     /translation="PMMTEPAPHAPPTPTPIAELSAVMPAYNEEEVLPFSIAEAVAAL
                     DEIAARWELLVVDDGSRDRTPEIVAEWVARNDRIRLLKQPMNLGYSRALIRGFREARY
                     EWVCYTDADAQFDLREVRKLYDQAGGVEMVVGYRTKRQDPSIRLLTSAVYNRLQGLVL
                     GVRVRDCNCALKLFRRDFLQSLPLDSDGFLIDAELYARTRRAGGRWREVGVTHRPREL
                     GRTTVRVATIVKTLRELWALRRSIARGETAAR"
BASE COUNT         2048 a         4361 c         4289 g         1855 t
ORIGIN      
        1 ggccgacctg ctgcagggcg acaacgcccc gcagccgtac gattcgtcgc tcgacggccg
       61 cctggcgttc tacatgaacg gcagggtcag cgatcgctgg aagctgaccg ccagcgccga
      121 tacccgcgag gggccggtgg aggatctgtt cagcaacttc ctcgacaagt cgcccgactc
      181 gttgttccgt cgcatcgatc ccgattatca ctacccgacg ttcggcgacg acagcgtcgt
      241 gcagcaaatg gcgccgaccc aaggcaagct ctacgtcaag gccagccgcg gcgagagcta
      301 cgggttgtgg ggcaacttca aggtcggcta cctggggaac gagctggccc aggtcgatcg
      361 cggcctgtac ggggccaacg cgcactacga gaccgaagcg acgacccgct tcggtgagcg
      421 acggatcgcc gtcgacgggt tcgccgccga acccggaacg atcgcgagct acgaagaatt
      481 ccgcggtacg ggcgggtcgc tctacttcct gcgccgccag gacatcctga ccggctccga
      541 gcgcgtgcgc atcgaggtgc gcgacaaggc ctcgggaatc gtcaccgggg tggtcaacct
      601 gcggcccaac accgactacg acatcgacta tctgcagggc cggctgctgc tgtccgagcc
      661 gctggcctcg acccccgacg acaacctgct ggttcgcacc agcggcctga gcggcgacga
      721 ggcgtatctg gtcgtgcgct acgagtacac cccggggttc gacgagctcg acgccgtcgc
      781 cgtcggcggc cagggccacg tctggttcac tgaccacctg cgactcggcc tgaccggcaa
      841 ctccaacgag gaagggagcg ccgacagcag tctcggcgcc gccgacctga ccttgcgcaa
      901 gagcgccgaa tcgtgggtca agctgcaggc cggtcggacc gacggtctga tccagagcgc
      961 gctgcggtcc gaggacggcg gattcgagtt ccagagcacc gacgacccgt cgttcatcga
     1021 cgccgacgcg gacgcgtacc gcgccgacct gagcgtcgga ctcggtgact tcttcgccgg
     1081 gcccgacggt cgccttacgc tctacgcgca gactctcgac gccggctact cgtcgccggg
     1141 ccaggccacg atcaaggaga ccgagctcta tggcggcacg ttccggatgc cggtgacgcg
     1201 ccgactctcg ctggcggcca agggcgatca gcggaccgaa gagctggggc tcgagagccg
     1261 ggcgctcgaa ttgaacgtcg gttatcggct gaccgacgag tggagcgtca gcgccggcgc
     1321 gcgcaacgat cggcgcgagg accgttcgcc cgtcattccc ctgacgcagg aagagggcga
     1381 gcggaccgac gcggtgctgc aggtggggtt cgatcccggt gccgcgtggc gcgcctacgg
     1441 cttcgtccag gatacggtcg agtcgagcgg caaccgtgac gacaacggcc gcatcggcgc
     1501 gggtggatcc tatcgcctgt cgaaacgctt caagatcgac ggagaggtct ccgacgggga
     1561 cctcgggccc ggcgggcggg tcggcaccag cttcctctac tcggagcgga cccatctcta
     1621 cctgaactac gcactcgaga acgagcggac cgacaacggc atgcggatcc gtcggggcac
     1681 gttggtgtcg ggcgtgaagc gcaggctgtc cgacagctcg agcgtctacc tcgaggagcg
     1741 ttaccaggac agcgggtcgc tgagcggcct gacgcatgcc accggaatcg acctcgtcgc
     1801 cggagaacgc tggaacctcg gcgccagcgc ggaactcggc acgctgatca actcccagac
     1861 ctccgccgag accgatcgcc gggcggccgg cgtgcgcctg ggttacggcg tggagacgat
     1921 gcaactgtcg accgcgatcg aatatcgccg ggacgaacag gagcagctcg atacgacgct
     1981 cgacgaacgg aaggtatggc tgttccgcaa caccttcaag ttccagttgt cgcccgactc
     2041 gcgggtcgtc ggcaagctca accattcgtt cagtgacagc tcgctcggcg aattctacga
     2101 gggcggctac accgaaggcg tggtcggcta cgcgtaccgt ccggtacggt acgaccggct
     2161 gagtgccctg gccaagtaca cctacttcta caacatgccg accgccgacc agctgggagc
     2221 ggattcgact ccgagcgagt acgtccagaa gagccacatc gccgcgctcg atctgtccta
     2281 cgatctgtcc gccgcctggt cgatcggcgg caagtacgcc tatcgcctgg ggcaggtcag
     2341 tctggatcgc gaacagccgg agttcttcga caacgcggcg cagctcgtcg tgttccgcgt
     2401 ggactggcag ttcctcgagg gctgggagag cctggccgag gttcgcgccc tcgagctccc
     2461 ggacctcggc cagcggcggg gcggtgcgct gttctcgatc tatcgttaca tcggcaagca
     2521 cctgaaggtc ggcgcgggct acaacttcac cgatttctcc gacgacctga ccgacctgag
     2581 ctacgaccac caaggcgtgt tcgtcaacct gatcgggacc atgtagcgcc cgccgcgctc
     2641 gcggcggccg gacctggtag actgtccgcg gcaaccggcg gagacggggg aggtctctcg
     2701 ccgactcgtt cgcgccgtgg gactcaggac tcgagggact tcgtcgttcg tcggcgcgct
     2761 ggccgtggcc ctgctggtgt cgtgccgagc gcccgacggc acggtcgggc cggaggaccg
     2821 gtcgttcgtc gagcgaccgg gcgaggggcc gaacgtcgtc gagatcgcga cgccgtggct
     2881 cgacggctgt ggctcgggct gcggcaacgc gtacctcgag ctcgcacgcc gcttcgccga
     2941 gcggggcgcg ccgatctcgg cggcggagct cgaccgccgg ctcgcggcgg cggaggccac
     3001 cgcggacgcc cctctgctcg ggctcggccg cgacgagctc gcacgtcgga tcgtcgccga
     3061 actgcgcatc ggctggatgc tcgacgaccg cgaacggcgt ggcctcgccg tggcgcagct
     3121 cgccgagcgt cgcggcgacg gcttcgtccg tcgcaagctc ttgttctggg accccctggt
     3181 cggcagcttc gaggggacgc tgctgcttcc cggcggcagc ggacgccacc cggcgatact
     3241 cggcctgcac ggccaccgcc gcgacgacga gacgttcgtc cgcgactact tcggtgagga
     3301 gctcgccgcg cgaggtttcg cggtgctcgt tccccgcctg cgcgccgacg actgctcgcg
     3361 cagcgaaaac cggatcgccc gcgaactact ggagcgcggc ttcacgctga tgggactgcg
     3421 ggtctacgag gcgctgcgca tggtcgacta cctcgacacg ctgccgcagg tcgacgcggg
     3481 ccgcatcgct ctgttcggcc actcgggcgg cagctcgctc gccaatctcc tgctgcgcgt
     3541 ctccgaccgc ttcgacgccg cggtcgtcga ccacgaggtc gacttccgca agcgccgcgg
     3601 cccgtgtcac ctccactgcg agacgatccc cgggatgttc ccgctcgcgg ccgacatcaa
     3661 cgaccgccgg acgctggcga ttccctggat gcgggccagc tacaagtacg tccgggaggg
     3721 ggagtcgggc caggtgatcg acttcctcga ccggcgactc gccctggtcg cgacgaccga
     3781 tccctagcgc cggcacgatt tctcggacgt tcgagccgtt gatgcgccgc atgagcgcga
     3841 tgaaacgagg gtcgtcgcgc agtgggtcga acagcgggtc gatgcgcaga aacggaatga
     3901 tccagccgga acggaggtcg accgccgcct ccaggtggtc gagggcgagc tcgtggtcac
     3961 ccgatgccag gtacgcctgg gccatctgcg tgtgacgttc tccacgcggg atcggtgccc
     4021 ggtcgatccg ttcgagctcc caggtccaga acgcccgcat cgccgcggcg gcgtcgccgg
     4081 tctccatcgc gtcgacgtcg gctgccgtgc cgccgagcag ccgcatgtgg gtccgagcct
     4141 gacgtgccgc gccggcgacg tcgcccatct gcagcgaaga gagcacgagg cagcgatggg
     4201 cccagaaatg gagcgggaac gactccaacg tcagccggga gtagccgatc gccgtgtcgt
     4261 agcgacgcgc gaggtaggcg taccagccga gatccgacat cacccgaggt gagagcggat
     4321 cgagctggat ggcgcgtcgg acggccccca gcgccccgtc gtgatcgccg atgaccgaga
     4381 accatgcggc gtagtcgtgc cacgccgagg ccgagcccgg attcaactcg acggcccgct
     4441 ggaacgcggc gccggccccg gccagatccc agtcgtaata gaacaggacg atggccagtg
     4501 catgatgggc ttcgtcgagc gtctcgtcca gcgcgagcgc cttgcgcgcc gccgtgcgca
     4561 tctgcgagaa ccgatccccg ggggaaaggt gtcgcagacg ctgcgccatc gccaggccgg
     4621 catacgcgtg tgcgaaaccg ggatcgacct cgatcgccgc gcggaatgcc acgtcggcac
     4681 cctcgggatc gatgccgccg ggccggctcg tcagctgccg ggcacgcagg tagtgctcgt
     4741 aggccgccgg cgacgtgacg gacgcctccg gctggcgatc cgcgggcggc agcagctcga
     4801 gaccgagcgc ctcggtgatc tggcggctca cctcttcttg tagcgcgagc agatcgaccg
     4861 tccggcggtc gtagctgccg gtccacacgt ggagctgatc gcgcacgcgg atgaggtgcg
     4921 ccccgatcct caaccggtcg ccgtcccggc gaaccgtgcc ccggaacaaa tagtcggcgt
     4981 cgagccgcgc cccgatctga tcgtcggagg tcccgctccc cttgaacctc ttggccgacg
     5041 accaggcgat gacgccgatc cggccggcgt agcgtcgccc gagctgggtg atcagctctt
     5101 cggtgaggcc gtcccaaacg aactcgtcct cgtcggcggc acccaggtat tcgaacggca
     5161 cgacggccag gatcggcacg gtcggcgacg cggtcgcacg accggcgaag ccgatgccgc
     5221 ggccgctgag gacgaggccg gtcaggccgg aggccgccac cacgaccacg agcgccaagc
     5281 cgacgagccg cccacgcttc gacggggccg ccggcgcggc gccaccgccg ttctcgacgg
     5341 gcgcgacgaa gcgatagccg agcttgggga tcgtctcgac gtagcacggg gattcggcgc
     5401 tgtcgcccag cgccgcgcgt acctggcgca cgcagtggtt gatgccctgg tcgaactcga
     5461 cgtgcgtacc ggacggccac agcgacctct ggatctcgtc ccgcgacacg atctgaccgg
     5521 aacgggcgac gagtaatgcg agaagccggg ccggttgggc ttgcaggggg atggcgcgtc
     5581 cgcggcgcct caggtcaccg cggtccggat cgaagtcgaa cagtccgaag cggacggtcc
     5641 gggatgtcga atgctcagac tgcatcctca gggtaaaccg cattgtacgt cggggtttcc
     5701 ggcccggcaa tcacgcggcc ccgaactcag tcacggccgc aaccccaacc tcctcaaaga
     5761 cctgccgcac tcagccacgg atcagcgtcg cctcatcccg cgttcatggt cggcggcgcg
     5821 gccacagccc atctgaccca gacctcggtg cttccggatc ccccggaaac cccagagggc
     5881 aagggagatg cagcatgaat cgaaacgaac gacggttttg gcccctcgtg gggctcgcga
     5941 tcgcactcgt cgcctcgccg tcctggtcgc aattcgtcga gccggatgtg acggtgatcc
     6001 gaacccacct gtcggccgac cccgccgaga gcttcggttg ggcggccgcc cggatcggcg
     6061 acctcgacgg cgacggcgcc ccggagtact tgatctccgc gatcttcaac gcacagaacg
     6121 ggttcaacgc cggccgggtg gtcctctact cgggcgcgga cggcagcgtg atgaacgagg
     6181 tgttcggcaa cccgggtgac cggctcggac accagctcgc ctccgccggg gacgtcaacg
     6241 gcgacggcgt gctcgactac gccctcggcg gtccgggatc ggttccggcg ccggggccgc
     6301 aaccggggcg cttgctggtc ctttcgggag ccgaccactc cgtgctgctc gacgtcgccg
     6361 gcccggggga tcggaccttc ttcggctacg acgtccatgc cgccggcgat ctgaacggcg
     6421 acgggttcga cgacctggtg gtcggcgccc cgcgggattc gacgagcttc gcgttcgccg
     6481 ggcgggccta cgcgatctcg ggccgggacg gtagcaccct ctggacctac gggggggaac
     6541 aaggcttcta cctggtcggc accggcgtgg ccggtatcgg cgacctgaac ggtgacggga
     6601 tccccgagca ggtcgcggcc ggattccagg ccggacacaa gttccagggc gaaggatacg
     6661 ttctctccgg tgccgacggg acggtgctcc acgtactcaa gcccaagaac gaaccgcgcc
     6721 agaacttcgg caacttcttc gtgcaaaacg cgggcgatac cgaccgggac ggtgtcgacg
     6781 acatcctgat cggcgatttc ggcatcccgt ccacgccggg gcgcgcctat ctctggtcgg
     6841 gggcggacac ggaagctcgc cagatcctcg acggcgccaa cgcggccgac ggcttcggca
     6901 tcgggcgagg cgtcggcgac gtcgacggcg acgggcacgc ggacctgttc ctcgccgcgt
     6961 tccacaacag cgagggcgcc ttccaggccg gcaagggcta cgtctattcg ggacgcaacc
     7021 tcaagcggat ccgggagatg accggaacgg tcgccggcgc gcagctcggg ttcgatgcgc
     7081 tgggcctcgg agacgtgaat gccgacggtc ggatcgactt catgctgacc ggtgtcggcg
     7141 tggcgcacgt gatcgcgggg aatcccgtgc cgtaagcgaa gcggttcgag gcggccggcg
     7201 cgacgccggc cgcctcgatc gaaacgggcg ttccagcccg tcaggggccg cagctccccg
     7261 tccccggaac ccgcgtgaag ccgccggttc cgacgcccca atcgccgacc gcggcgcccg
     7321 taccctgcgt gccgcggtag acgtagacca gcacctgtcc cggggccggc tgcagctcgt
     7381 cctcgaatcc acgcgtgtcg gtgtcgggcg acacctgttc gagacaggtc accggaccga
     7441 gcgcgatcgt gccgtcgtcg tagccgacgt tcgcaaggtc gccgcgcacg atgtcgtagc
     7501 gcacgtaacg cgggtcggcg tcccaggtca cgacgctcgg ggcgctcttg ccggcttcga
     7561 tccggctccc gcgctgctgg tcgaccagat acagctcgga gccgtagtcc ggattggctc
     7621 cggcccaatc gcccgcggcg gtgaagacgg cccgccgtcc tgtcccgtcc accgccatgt
     7681 tgcagtcggg gtcgacgagc gagcaggcca agtcggtcgg gccgcggcgg atcccgccga
     7741 cccgctcgat cgttccgctc gcgatctcgt agcgatacgg ttcatgacgt cgctgcggat
     7801 tctcgtcgaa gaacggcacg ctcgagttga agtagatcca gttgccgtcc ttgctgatcc
     7861 gcggtcggga actcgagccg acggtcgtca cggtgacttg atcgatcgtc gccgcctgca
     7921 cgtcgtagcg gaagatctcg gagttgccgt cgggattcgt accgagtgga tcgttgctcc
     7981 cggcccagac gatctgctgc cccgaccccg agatgtccgg ccaatgggtc tcgaacaacc
     8041 cggccgacac gatctgctgg aagccgctgc cgtccgtccg gatccgggcg atgccctggt
     8101 tctcgaagac gacccattct ccgttggcgt cgatacgcgg gtagaagttg ccgctgcccg
     8161 ccaggttggt gaacttcgcg aactcgctgc cgtctggacg cacgcggtag agctcctgat
     8221 cgaacaggcc gacgaagacg acccagctgc cattggccgc gatcaccgga aagaacgcgc
     8281 cctggcccgg gacgaacggg cccgaaagaa cacggagacc cgtcccgtcg gcctcgacgg
     8341 cgtagacctg tgggttgccc agatcgttgc cgatgccgag cggatcttcg taggcctgga
     8401 acacgatcgt ctccccgtcg gccgtgatcg acttggcctc gacctcgaac gggacgacca
     8461 actggtgctc tccgtcggcg ggatcgacgc gcagcatccc gccccccgag agcgaataga
     8521 cgattcgcga gccgtcgggc gtgatctccg gcgcgaagac gtagggatcg tcccccgtca
     8581 gctcggcgag cacgacctcg ttgtcgccgc ggaagtcgat cgcgcgcagg ctgtcggtcg
     8641 tccagcgctg gtagaacgcg atccgcgtgc cgtcgcccga gaccaccggg tcgacgttgg
     8701 agcggccggt cgtcgaggtg acagccgcga cgatgctccc gtcgagcgat gcctgccaga
     8761 tctcgtcgtt tccctccgga ttgtcgctcg gatcgatccg gttgtcggaa aagtagacga
     8821 acgaaccgtc ggccgtgatc gagggagagc ttccctgctc cgacagctcg atgaacccca
     8881 tccacggatc gaccacgacc gcggagctgt tcatctccga cgcggcgtgc agaccgttcc
     8941 ccgaaaggta cgaaaagatc ccgccttcga ccggctggag gccgctgccg tcgtcgagaa
     9001 tgacgaacaa gccgggggcg tgatgaaaca gcacgaccgt cccggcgtcg ctgatgtcca
     9061 cgtcgagatg ggtgccggtc cagtgcgatc cgggcgactg atcggccgag gtcgcggccg
     9121 tcagctgaat cggcgagctc ccgtcgcgat cgaccgcgaa gacctgcggc acgcgatccg
     9181 ggttcgcgcc gagcgggtcg gtgtcggcga tgaacgcgac gcgatcgcca ttgccgctga
     9241 tcgccacgcg gtagatccac gaagtgttgg ccgggccgac gtggttcgtc agctgggcca
     9301 ggccggaccc gtccggctgg atcacgaaga tctcgagacc ctcgtcgtgg ttctggccgg
     9361 tgagatcggc aggggagacg aacgcgagcc actccccgtc ggcgctgacg gcgaccgagc
     9421 ggcgcatcca gctcacgccg tccgggtcct cgacgagggc cgagccgatc ccggtcaccg
     9481 cgttccacgc gccgagttga aagacgccgc ccgggttccc gccgaacgcg tccgcattga
     9541 cgttggcgaa tacctgctcg ccggcgtcgt cgagcgccgg cgtcaacgcg gtctcgcggt
     9601 cgagatcggt cagctggagc aagacctggg cgtcgaccca ccccgtggcg aggcagagga
     9661 cagcgagaaa gaccgcgaat cgttctttcg ccatgacccc tccttgttgg tcttgaatta
     9721 cagcacttat ataccgcgtt ggagggtagg ggcaagacca atcccgccgg cgggctcagc
     9781 cttcgagcaa ctcgagccgt ttctgcgctt cgcgcttgcc cagaccgcgg atctcgacca
     9841 gagccgccag ctgctcgtcc cgcggcctgg atttgccctg ctcccagttg tagaccgtct
     9901 gtccggtgac accgacgagg cgggcataat cggcggcgga caggtcgagc ttcttccggt
     9961 gggtctccaa tccgcgccgc gagaaccgct tgccctcgac gaccgcggcg ggagccgggc
    10021 tcgtcgcccg cttgcgttct tgtcgctcga ggtaggcgac ctgcttcgac aggccggcga
    10081 cctgacgctt caattcggcg atatcccgcc ggtattgcgc ggtggctctg cgcaacaccg
    10141 cggtctcgga cttggtcgcc ttgcgggaaa gacgggtgat ctcctgtttc agcaccgagg
    10201 cgatgttcga catgacggcc ctttccaact ccggtggggt caactcgttt tcttcggacc
    10261 catgagtata aacggtcggc gccggcgcga cacctggtca gcgccccggt ggaagccggc
    10321 tcatcactcc cggcgccggg cgcgcctcga ggcagcgcgc catcgtgatc tctcggaccg
    10381 gctcgccgcg cgcacgtacg acgaccggcg ggagcgggtg aacctcccgg cagatgtcgg
    10441 cgagcgcgac gagcgtttcg cgacggccgg tgacccagac caggtcgacg ccgtcgaacc
    10501 gttccggcgg gaaccagtag agcgcggcca ggccgtggac cccgcggttg acgtcggcga
    10561 ggtgcaccgg cagcgtgcca cccgccgcga acgcgacgag gtggaccatc gagtagctct
    10621 ccgatgcgac gatctctccc gggcgcagca cgtcccgcag cactgcggcc gtctcgtcgt
    10681 agccgaccat cctcgcgagc ttgtcggccg gaatcgcggc gagcggaccg ccgccggccg
    10741 tgcgacagcg cgcgatgaac gcggccggat cggcgcagat cgcgagcacg agcgccagca
    10801 gcaccaccga ggtcgcggcg ccggccagcg tccagatctt ccatccgcga aagcgatgga
    10861 agacgagcag cagcgccgac aggacgagcg tcggcgcgcc ccagtgcgcc gcgacgcgcc
    10921 cgcgcagggc gaacgcggcg aacagcacga acgacgcgcc cgcgacccag cggacggcga
    10981 cccagcccgg ttcgcggcgc gctcgcgcac gccaccacgc gacggcgaac gccacgccga
    11041 cgaacgacga cagtgcgagg ttagccgcca cgaactcgac gacgtggcgg acggagaacc
    11101 gcgcggcgtc gggaacccgc ccgctctgga acgtgacgtt gtcccagccg tggcgcgccc
    11161 cccagatcca caccggtgcg gtcagcatca gcgacgtcgc gccggcgagc cacggcgtga
    11221 gcgtgcggag ctgcgcccgg acgtgacgca tgcggagcag gcgcacgacg gcgatcagca
    11281 ccgccaccat cgggaacttg gcgaggaagc cgagaccggc ggcgaggccg aagccccacc
    11341 atccgcggcg atcgccctgg gcgatggcga ccgccgccca cgccgctccg acgaagcagg
    11401 cggtcatcac gatgtcggtc gagacgtacg acgagccgaa caggacgagc ggtgtggcgt
    11461 ggagcacgag gtaggcgagc gccgcctcgc cgacgccgcc gccgagccgc cgggcgagcg
    11521 gcaacatcag cagggcgatc gcgaggctcg cgacgatcgc cggcgcccgg accgcgagcg
    11581 cggtctcgcc caacacgagc cggaacggca ccatcgacca cagcaccagc ggcggttggt
    11641 cgaacgtcga ccacgccggg tgtcgcgaca ggtcccagta gtacgcctcg tcgccgctga
    11701 gcggggtcac gacggcgacc gccgcgtgca gcgcggtcgc ccccgcgagc agcagccacc
    11761 accggccggt cgacgggccg ctccgctcgc cgtcggcgat cacctcgcgg cggtctcgcc
    11821 cctcgcgatc gagcgccgca atgcccacag ctcgcgcaac gtcttcacga tcgtcgctac
    11881 gcggacggtc gtccgaccca actcgcgcgg acggtgggtc accccgacct cgcgccaacg
    11941 acctcccgcc cggcgcgtgc gcgcgtacag ctcggcatcg atcagaaagc cgtcgctgtc
    12001 gagcgggagc gactggagga agtcccggcg gaacagcttg agcgcgcaat tgcagtcgcg
    12061 cacgcgaacg ccgagcacga gcccctgcaa gcggttgtag accgcggacg tcagcagccg
    12121 gatcgacggg tcctgtcgct tcgtgcgata accgacgacc atctcgacac cgccggcctg
    12181 gtcgtacagc ttgcgcacct cgcggaggtc gaactgcgcg tcggcatcgg tgtaacaaac
    12241 ccactcgtag cgcgcttcgc ggaagccgcg gatcagcgcc cggctgtagc ccaggttcat
    12301 cggctgcttc agcagccgga tgcggtcgtt cctcgcgacc cattccgcca cgatctcggg
    12361 cgtgcggtcc cgcgagccgt cgtcaacgac cagtagctcc cagcgagcgg cgatctcgtc
    12421 gagcgccgcg accgcctcgg cgatcgagaa cggcaagacc tcttcttcgt tgtaggccgg
    12481 catgaccgcc gacagctccg cgatcggggt cggcgtcggc ggtgcgtgcg gagcgggttc
    12541 ggtcatcatc gga
//
LOCUS       DASPGN010000122         3556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1661122, whole genome shotgun sequence.
ACCESSION   DASPGN010000122 DASPGN010000000
VERSION     DASPGN010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661122"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..2020)
                     /locus_tag="VD788_03390"
     CDS             complement(74..2020)
                     /locus_tag="VD788_03390"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HXV75336.1"
                     /translation="MRADERNEWLEADGLGGFASGTASGIRTRRYHALLLVATTPPSG
                     RYVLVNGIEAWARTADGDEALTSQRYAPGVVHPDGAARIESFASAPWPSWVFRLRDGC
                     RIRFDLFVPHETSSVVLSWRADRVKPPLELFVRPLLSGRDPHALHRENTAFRFEPAPL
                     PGGGQRWAPYPDVPAIYVTGNGEYDHSPDWYRNFVYEQERLRGLDFIEDLASPGVFRW
                     DLARGPASLSFSADEARLPGGAGVERERRAERRRRRRFPSELDRSADAYLVRRGTGKT
                     IVAGYPWFGDWGRDTFISLRGLCLTTGRLDDAGRILREWARAVSNGMLPNRFPDRGEA
                     PEYNSVDASLWYVIAVHEFLEAVRRERGAPSRLASELREAVDAILEGYARGTRYRIGM
                     DRDGLLASGEPGVQLTWMDAKVGDRVITPRTGKPVEVQALWLNALAIGASWNARWRGV
                     LERGSRSLTERFWNPANGMLYDVVDVDHVAGSHDAAVRPNQILTVGGLPRCVLDSGRA
                     RSVVDGVERRLLTPLGLRSLAPDEAGYAARYEGGVAERDGAYHQGTVWPWLLGPFVEA
                     WVRVRGGSTDARRQASRRFLAPVERHLRDAGSGHVSEITDAEDPYTPRGCPFQAWSLG
                     ELLRLSRQTLEVATETGPVAGVAK"
     gene            complement(2017..3054)
                     /locus_tag="VD788_03395"
     CDS             complement(2017..3054)
                     /locus_tag="VD788_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV75337.1"
                     /translation="MTGRSRHSGILAVAIAFTLSLVHAAESSAPVRAVESVGLTVADL
                     ERSVRFFTEVLDFERIARAELDGEAFEAQTGVFGSRARVERLALGREHVELTEYLAPA
                     GRPLPADSRGNDRWFQHIAIIVSDMDAAYAKLREHGVRHLSPGPQTLPSWNPRAGGIR
                     AFYFKDPDGHALELLWFPPGKGNVRWQRADGKLFLGIDHTAIVVADTESSVRFYRDVL
                     GLREEGRGENYGPEQERLNNVFGARLRITTLRAADGPGIELLEYLSPPDGRPYPADAR
                     ANDLVHWETVLDVADPAAVAVELQGAGGKWVSSGVVPGAAESPFSESLLARDPTGHAL
                     RLVRRRAEPAR"
     gene            complement(3071..>3556)
                     /locus_tag="VD788_03400"
     CDS             complement(3071..>3556)
                     /locus_tag="VD788_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HXV75338.1"
                     /translation="VAGLMIAWALLGNVLAQPEIRFVATLPTGGAAAALAAEFVISFV
                     TMLVVLVSTNGSSRGRYTGLIVGGLVAIYITFEQPLSGMSMNPARSFASALPSGAWHG
                     FWIYVVAPLAGMLAAAEIHPRLRGASAVRCAKLNHDNDQPCIFRCGYCVHGASGADRA
                     A"
BASE COUNT          565 a         1272 c         1231 g          488 t
ORIGIN      
        1 cctggaggcg gacttgggta accggtacga cgctgccgtt catcgtcaac tccttcgcgg
       61 gggttccccg cactcatttc gccacgccgg cgaccggccc ggtctccgtc gcgacctcga
      121 gcgtctgacg cgacagtcgc agcagctcgc cgagcgacca agcctgaaac ggacatccgc
      181 gcggtgtgta tgggtcctcg gcgtcggtga tctccgaaac gtgtcccgac cctgcgtccc
      241 gcaggtgccg ctcgaccggc gccaagaagc ggcgtgaggc ctgccggcga gcgtccgtcg
      301 acccaccgcg gacccgcacc caggcctcga cgaagggtcc gagcagccaa ggccaaacgg
      361 tgccttggtg atacgcgccg tcgcgctcgg cgaccccgcc ctcgtaacgg gcggcgtagc
      421 ccgcttcgtc gggggcgagc gatcgcagac cgagtggcgt caacagccgg cgctcgacgc
      481 cgtcgaccac gctccgcgcg cggccggagt ccagcacgca gcgcggcaac cctcccaccg
      541 tcaggatctg gttcggacgg accgcggcgt cgtgggaccc ggcgacgtga tcgacgtcga
      601 cgacgtcgta cagcatcccg ttcgccggat tccagaaccg ctcggtcagc gaccgtgatc
      661 cgcgctcgag cacgccccgc cagcgggcgt tccacgacgc gccgatcgcc aacgcgttca
      721 gccagagcgc ctgaacttcg accggtttgc ccgttcgcgg cgtgatcacg cggtcgccga
      781 ccttggcgtc catccacgtc agctgcacac ccggttcgcc cgaggcgagc aggccgtcgc
      841 ggtccatccc gatccggtag cgtgtcccgc gggcgtagcc ttcgaggatc gcgtcgaccg
      901 cctcgcgcag ctcgctcgcc aaacgcgacg gtgcgccgcg ctcccggcgg acggcctcga
      961 ggaactcgtg gacggcgatc acgtaccaca gcgatgcgtc gaccgagttg tactccggcg
     1021 cctcgccgcg gtcgggaaac cgattgggca gcatgccgtt cgacacggct cgcgcccact
     1081 cgcggaggat acgaccggcg tcgtcgagac gtccggtggt cagacacagt ccgcgcagcg
     1141 agatgaacgt atcgcgaccc cagtcgccga accacgggta gccggcgacg atcgtcttgc
     1201 ccgtgccccg gcgcaccaag taggcgtcgg cgctgcggtc gagctccgac gggaaccgcc
     1261 ggcgccgccg gcgctccgcg cgacgctccc gctcgacgcc ggccccgccg ggcaagcgcg
     1321 cctcgtccgc cgagaagctc aggctggccg ggccgcgcgc gagatcccag cgaaacaccc
     1381 cgggcgacgc caagtcttcg atgaaatcga gaccgcgcag ccgctcctgt tcgtagacga
     1441 agttgcgata ccagtcgggc gagtggtcgt attcgccgtt gccggtgacg tagatcgcgg
     1501 gcacgtcggg atacggtgcc cagcgctgcc cgccacccgg aagcggcgca ggctcgaagc
     1561 ggaaggccgt gttctcgcgg tgcagggcgt ggggatcgcg accggagagg agcggtcgca
     1621 cgaacagctc gagcggtggc ttcacgcggt cggcgcgcca gctcagtacg acggacgatg
     1681 tctcgtgcgg aacgaacagg tcgaagcgga tccggcatcc gtcgcgcagg cggaagaccc
     1741 agctcggcca cggcgcgctg gcgaacgact cgatgcgcgc cgcaccgtcg ggatggacca
     1801 ccccgggtgc atagcgctgg gaggtgagcg cctcgtcgcc gtccgccgtt cgcgcccagg
     1861 cttcgatgcc gttgaccaag acgtagcggc cggacggggg tgtcgtcgca accagcagta
     1921 gcgcgtggta gcgccgcgtg cggatgcccg aggccgtgcc ggaagcgaag ccgccgagcc
     1981 cgtcggcttc gagccactcg ttccgttcgt cggccctcat cgcgccggct ccgctcgccg
     2041 cctgacgaga cgcagcgcgt ggccggtcgg gtcccgcgcc agcagcgact cggagaacgg
     2101 cgactcggcc gcgcccggca ccacaccgga cgagacccac ttgccgccgg caccctgcaa
     2161 ctcgacggcg accgccgccg gatcggcgac gtcgagcacc gtttcccagt gcacgaggtc
     2221 gttggcgcgg gcgtcggccg ggtacggccg tccgtccggc ggtgacagat actcgagcag
     2281 ctcgatgccg ggaccatccg cggcacgcaa ggtcgtgatg cgcagccgcg ccccgaaaac
     2341 gttgttcagc cgctcctgct cgggaccgta gttctccccg cgaccctcct cgcgaagtcc
     2401 gagcacgtcg cggtagaagc ggacactcga ttccgtgtcg gcgacgacga tcgccgtgtg
     2461 gtcgatgccg aggaacagct tgccgtccgc gcgttgccag cggacgttgc ctttgccggg
     2521 cgggaaccac agcagctcga gcgcgtggcc gtcggggtct ttgaaataga aggcgcggat
     2581 cccgccggcg cgggggttcc acgacgggag ggtctgcggg cccggcgaca gatgccgtac
     2641 gccgtgctcg cgcagcttgg cgtaggcggc gtccatgtcg ctgacgatga tcgcaatgtg
     2701 ctggaaccac cggtcgtttc ctcgcgaatc ggcgggcagt ggccggcccg cgggtgccag
     2761 gtactccgtc agctcgacgt gctcgcgccc gagagccaac cgttcgactc gcgcgcggga
     2821 accgaacacg ccggtttgcg cctcgaacgc ctcgccgtcg agctccgccc gtgcgatccg
     2881 ctcgaagtcg agcacctcgg tgaagaaacg caccgaacgc tcgagatcgg cgacggtcag
     2941 accgaccgat tcgaccgcgc ggaccggcgc cgacgactcg gcggcgtgaa ccagcgacag
     3001 ggtgaacgcg atcgcgaccg cgagaatccc ggaatgacgg ctccgacctg tcatcgctcg
     3061 gcctccggag ctaggcggcg cggtccgcgc cgctcgcgcc gtgaacacag tatccacagc
     3121 ggaagatgca gggctggtcg ttgtcgtgat tcagcttggc gcagcgcacc gcgctcgcgc
     3181 cgcgcagccg tgggtggatc tcggcggcgg cgagcatccc cgccagcggg gcgacgacgt
     3241 agatccagaa gccgtgccac gcgccggacg gcagcgccga cgcgaagctc cgcgccgggt
     3301 tcatgctcat tcccgacaga ggttgctcga aggtgatgta gatcgcgacg agcccaccga
     3361 cgatcaaccc ggtgtagcgc ccgcgcgacg agccgttggt cgacaccaag acgacgagca
     3421 tcgtaacgaa cgagatgacg aactcggcgg cgagcgcggc ggcggctcct cccgtcggca
     3481 gcgtcgcgac gaaacggatt tcgggctgtg ccagcacgtt gcccagcagg gcccaggcga
     3541 tcatcagtcc ggccac
//
LOCUS       DASPGN010000123         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1192531, whole genome shotgun sequence.
ACCESSION   DASPGN010000123 DASPGN010000000
VERSION     DASPGN010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192531"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VD788_03405"
     CDS             <1..233
                     /locus_tag="VD788_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75339.1"
                     /translation="ATSLNYHVLRSADGADFVEMTDCLVGSDPSSRSWTEIGSPVAGA
                     IFHYLVRAVNDCPAGLGPLGPPGTSRVATSCP"
     gene            complement(230..2152)
                     /locus_tag="VD788_03410"
     CDS             complement(230..2152)
                     /locus_tag="VD788_03410"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75340.1"
                     /translation="MLNRARAFDPGDPALPVAGRGVLSRVRPSRRSRVRATVLGAVHL
                     VILLHVAHFLLAGRTLSPVEPSESMYTLELGQVNAGFVFFIAALVSTLIFGRFFCGWG
                     CHLVALQDLCAWMMKRIGIRPRPFRSRLLLLGPLIVALYMFAWPTFKRVVLDDSPSAF
                     PGFSNHLMTSGFWDTFPGPLFALLTLASCGFAAVYLLGAKGFCTYGCPYGALFGVADR
                     FAFGRILVSDACDQCGHCTVTCTSNVRVHQEVRDHGRVIDPGCMKCTDCVSVCPKGAL
                     RFGFAWPRLRARTPRPRPPGRLSWPHELLVAAVALVATLAFRGLYDGPPLLMAAGLGA
                     MTAFVALQVVRLIAGERDLRLQSLWLRKSGRFTAAGRAFVVLTTLWLVFTAHSAFVQW
                     HRAEGRRLLDATEVTRAEVFDGSWWTRRPSARHERARSRMADHFAAADRWGLGDTVDV
                     KLGLAWDRLLSADPAGAEGYLDQALALAPDNPALWSDLFELSLIRGRPDPALVAVERI
                     EGLGGRLPAAGHCKLADLLANSDRADLAIGHYLRCLEHSPAELSVRYNLGGLLRRAGR
                     LDEAVVQLEQARALAPNDADTHVELGLAYAATGRDREAIEAFTRAIELAPDRPESRLH
                     LPRLIDELRGRSAARP"
     gene            2283..>2583
                     /locus_tag="VD788_03415"
     CDS             2283..>2583
                     /locus_tag="VD788_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75341.1"
                     /translation="MMRKLLLSLSVVVWIAPCQADVATDTALGHIDFPNSGSADAQAP
                     FVRGVLLLHSFEYEDAREEFRRAQAIDPRFSLAYWGEAMTHNHPLWREQDREAALA"
BASE COUNT          429 a          895 c          918 g          341 t
ORIGIN      
        1 gggcaacctc gttgaactac cacgtcctgc ggtcggccga cggcgcggac ttcgtcgaga
       61 tgaccgattg cctggtcggt tccgacccgt cgagccgctc gtggaccgag atcggttcgc
      121 cggtcgccgg cgcgatcttc cattacctgg ttcgcgccgt caacgactgc cccgccggac
      181 tggggccgct aggcccgccg ggcacctcac gagtcgcaac ctcctgtccc tagggtcgcg
      241 cagcggaccg gccgcgcagt tcgtcgatca gccgggggag gtggagtcgg gattcgggtc
      301 gatcgggcgc cagttcgatc gcgcgcgtaa acgcctcgat cgcttcgcgg tcgcgacccg
      361 tcgcggcgta ggccagcccc agttcgacgt gggtgtcggc gtcgttcggc gccagcgcgc
      421 gcgcctgctc gagctgcacg acggcctcgt cgaggcggcc ggcccggcgg agcagccccc
      481 cgaggttgta gcgcaccgac agctcggccg gactgtgctc gagacatcgc aggtagtgcc
      541 cgatcgcgag gtcggcgcgg tcgctgttgg ccaacaggtc ggcgagcttg cagtggccgg
      601 cggccggcag acgcccaccg agcccctcga tccgctcgac cgcgacgagc gccgggtcgg
      661 gccggccccg gatcaacgac agctcgaaca ggtcgctcca cagcgcgggg ttgtccggag
      721 ccagtgccaa cgcttggtcc aggtagccct cggcgccggc cgggtcggcg gacagcagtc
      781 gatcccaggc cagaccgagc ttgacgtcga ccgtgtcgcc gagcccccag cggtcggccg
      841 ccgcgaaatg gtcggccatc cgtgaccggg cgcgttcgtg acgcgccgac ggtcggcgcg
      901 tccaccagct gccgtcgaac acttcggccc gcgtcacctc ggtcgcgtcg agcagtcgac
      961 gcccctcggc tcggtgccac tgcacgaacg cgctgtgggc agtgaacacg agccacagcg
     1021 tcgtcagcac gacgaaggcg cggccggccg cggtgaacct cccggacttg cgcaaccaca
     1081 agctctgcag ccgcagatcg cgctcgcccg cgatcaaccg gacgacctgc aaggcgacga
     1141 acgcggtcat cgcacccagc ccggccgcca tcagcagcgg cggtccgtcg tacaacccgc
     1201 ggaacgcgag cgtcgcgacg agcgcgaccg ccgcgacgag cagctcgtgc ggccagctca
     1261 acctgcccgg cgggcgcggg cgcggcgtgc gggcgcgcag cctgggccag gcgaagccga
     1321 aacgcagcgc gcccttcgga cagacgctga cgcaatcggt acacttcatg cacccgggat
     1381 cgatcacccg gccgtgatcc cgcacctcct ggtggacgcg gacgttcgac gtgcaggtga
     1441 ccgtgcagtg cccgcactgg tcgcaggcgt cggagacgag gatccgaccg aaggcgaagc
     1501 ggtctgcgac gccgaacagc gctccgtagg gacagccgta tgtgcaaaac cccttcgccc
     1561 ccagcagata gacggcggcg aatccgcagc tcgcgagcgt cagcagcgcg aacagcggtc
     1621 cgggaaaggt gtcccagaac ccgctcgtca tcaggtgatt gctgaatccg gggaaggccg
     1681 acggcgagtc gtcgagcacg acccgcttga acgtcggcca cgcgaacatg tagagcgcga
     1741 cgatcagcgg accgagcagc agcaggcggg aacggaacgg ccgcggccgg ataccgattc
     1801 gcttcatcat ccaggcacaa aggtcctgca gggccacgag gtggcagccc cagccgcaga
     1861 agaaacggcc gaagatcaac gtggaaacca gcgcggcgat gaagaagacg aagccggcgt
     1921 tgacctggcc gagctcgagc gtgtacatcg actcggacgg ctcgaccggg gacagtgtgc
     1981 ggccggcgag gagaaagtgc gcgacgtgga gcagaatcac caggtggacc gcgccgagca
     2041 cggtcgcgcg cacccgcgat cgtcgcgacg gtcggacgcg ggacagcacg ccgcgtccgg
     2101 cgacggggag cgccggatcc ccgggatcga aggcgcgtgc ccggttcagc acgccggtat
     2161 cggggtcggc tcgggcatga tccctccgtc gatgcgccgc gttcgcatcc gagtgtagct
     2221 cagctgcgcg gtttgacacg cgccgggccg gcatggatac tcggcgcgcg gagggatccc
     2281 cgatgatgcg gaagctgctg ttgtcgttgt cggtcgtggt ctggatcgcg ccgtgccagg
     2341 cggacgtcgc cacggacacg gcgctcggcc acatcgattt cccgaacagc gggagcgccg
     2401 acgcccaagc tccgttcgtc cgcggggtgc tgctgttgca cagcttcgag tacgaggacg
     2461 cccgcgagga attccggcgt gcgcaggcga tcgacccgcg gttcagcctg gcctattggg
     2521 gcgaggcgat gacccacaac cacccgctgt ggcgcgagca ggatcgtgaa gccgcgctgg
     2581 cgg
//
LOCUS       DASPGN010000124         6764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_138718, whole genome shotgun sequence.
ACCESSION   DASPGN010000124 DASPGN010000000
VERSION     DASPGN010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138718"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VD788_03420"
     CDS             <1..1186
                     /locus_tag="VD788_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HXV75342.1"
                     /translation="VPVGCYLSGGIDSCSILGMASGAQQSPVKAFTISFDDDDYDEAE
                     IATEMARSVNADHDLIRVGADELYGDNFLRTIWHAERTFYNTLGVAKWCMSRHVHRAG
                     YKSVITGEGSDELFGGYPALKRDMFLHGLEDESDETRAAYEAALYESNRLFRGAILAE
                     KQERHPALEELCGFTPSWIQPWLATLPVARSLLSNAAHEELADYDPVAEIASKLEPGA
                     LRGRHALDKAQYTWSKTMLEGQILNWGGDRVDMANSMESRPAFLDHNVAQAAVDVPPR
                     LRIRGTTEKWVLREAVRGVLPDALYTREKFAFMAPPAHTDRRKRRRVERLIEGYLNAA
                     SVEEAGLLDPARVAAFVDGWRRDGDPVSLIRKDGLLNHLLCLQVLHRQFIAADAPVAL
                     VG"
     gene            1223..1966
                     /locus_tag="VD788_03425"
     CDS             1223..1966
                     /locus_tag="VD788_03425"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HXV75343.1"
                     /translation="MKSSDETDPKFLNIDPLRKSYTERIVENVEQIEALRARNVALLC
                     IDLQYLDAAPGHGVFREAEKSGVPLEAQAFYFSTLEHTVLPNVRRLQDVFRDHRLEVI
                     HVRICSLTRDGRDRSTGHRRLGLLAAPGSREADFIERVAPLGDEIVINKTASGVFNST
                     NIEYVLRNIGVDSLFVTGVYTDECISTTVRDANDRGFFVTVIEDGCATVTPDRHDFTI
                     ATLRDRYTRVLTTERAIDEISRHVDADAS"
     gene            2017..2529
                     /locus_tag="VD788_03430"
     CDS             2017..2529
                     /locus_tag="VD788_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75344.1"
                     /translation="MGSSADLRSAIDWPRAALLVLLGWWTARIVLGTDWLFLDLVNLA
                     FHEAGHLFFGFAGSTVHYLGGTLGQLIVPGGLVAYFLVRRRQPFAAAFCLWWAGESLA
                     NVSVYMADARELALPLVGGGDHDWNELFFRCGMLTEPAVARISGLTRLAAVGVMLSAL
                     GWGSALAFNR"
     gene            complement(2557..3999)
                     /locus_tag="VD788_03435"
     CDS             complement(2557..3999)
                     /locus_tag="VD788_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75345.1"
                     /translation="MTTGAPVDQIPDEANATQVARPPGRTGTIDTEIRTGSPVGASTS
                     AGPKGAKPLAVEGFFQSLAQGLGETYLGAAMVWLGAGGLVLGLVGALPTAATALSQVV
                     AARMAGGVHGARAFIARSWLLQALALALLGGLALLRAPFAIPLVFGGAVLSWFAAGLS
                     VPSWTGLVSRVVPRERNGWFFGLRGAVQQVGVVSAILGGGAILSLAEREGRVGLGFVV
                     VFGLAGLFRAIGSGFLLAVPERGRPRRGTPLRAIGTVLRGSRKFRRLAIYLWAFHFAA
                     WVAAPFFLPYMLRELRFSYGQVGLLVAVPAIVKSMTMRRWGTLADRIGPGPLLRGIGW
                     FVLPVSSLWLISGEFWWIALAQFYAGLAWGGFELAQASALLQTTRGREDTVAFFNLVD
                     GSAMILGSVVGGLTVTFAGRYLGVGFLAAIAASTVLRALVNLSLLRRVRRIGRPEWSH
                     RAIPLRLWGVRASRGATFRPWGGLPPAADE"
     gene            complement(4037..4300)
                     /locus_tag="VD788_03440"
     CDS             complement(4037..4300)
                     /locus_tag="VD788_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75346.1"
                     /translation="MPTSDTNSTQPREREVTLEGVLIARRWKAAGQVAAYALNTLDER
                     EYLIDDPRFDGRVLFEHLRKRVRVTGTVIGNRVVRVASIGNSD"
     gene            complement(4399..5484)
                     /locus_tag="VD788_03445"
     CDS             complement(4399..5484)
                     /locus_tag="VD788_03445"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV75347.1"
                     /translation="MKRRVFAPGGGAQDPRVTAVETVSRAMRPVLDKIRAVAPTRTTV
                     LLTGETGTGKGVLARLIHELSNRAAGPFVAVHCGAMPDTLLESELFGHEKGAFTGAIR
                     RKPGKFEGAAGGTLFLDEVGTISSSAQIKLLDVLQQRRLVRVGGETEVEVDVRLIAAT
                     NADLGQMQRQGTFRPDLYYRLNVFPIAIPPLRERPEDIPALVERFIENCNTYLPKGVG
                     QLSPDVSHAFQSYAWPGNVRELENVVERAHILALGETIGAELIPPEIMAAGGSGAAAD
                     AAARGSLADARRAAVEIAEQRYLSELLARFHGRIDASATAAGVTTRQLHKLLTRYGIK
                     KEWFKSDRIVPTRETKAETPVPKPTDD"
     gene            complement(5510..6577)
                     /locus_tag="VD788_03450"
     CDS             complement(5510..6577)
                     /locus_tag="VD788_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HXV75348.1"
                     /translation="MRISALEAWQVTMRLAQPYSIAYESVSQATNVFLRVETNRGTVG
                     YGCAAPDPQVTGEQPAGVLAALNGIARAELTGSDPLRHAMLMMRAKKSLADFPSARAA
                     IDMALFDILGKVAGLPVWRLLGGYRRRIRTSVTIGILPQDETVAAARDYLARGFSCLK
                     MKGGTDVEQDIARVLAVRRAVGPEIELRFDANQGYTVDQSLRFVDATRSAALELLEQP
                     TPRDEIHELGRVSSKAALPMMADESLMTLRDAFRLARRDLADMVNVKLMKAGGIAEAL
                     QINAVARAARLEVMVGCMDEAALGIAAALQFALARPNVAYADLDGHLDLIDDPSAGSV
                     RLEHGTVIAKEDPGLGVCLRE"
     gene            complement(6585..>6764)
                     /locus_tag="VD788_03455"
     CDS             complement(6585..>6764)
                     /locus_tag="VD788_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HXV75349.1"
                     /translation="SVGWYAYTDKISPSGVMGDPTKATAEKGVKLWELMTRRLVELIE
                     DLKRLSLDEIYQRRY"
BASE COUNT         1047 a         2423 c         2277 g         1017 t
ORIGIN      
        1 cgtgccggtc ggctgctacc tgtcgggcgg gatcgacagc tgttcgatcc tcggcatggc
       61 ctccggtgca cagcagtcgc cggtcaaggc gttcaccatc tcgttcgacg acgacgacta
      121 cgacgaggcc gagatcgcga cggagatggc gcgcagcgtc aacgctgacc acgacctgat
      181 ccgggtcggc gccgacgagc tgtacggcga caacttcctg cggacgatct ggcacgccga
      241 gcgcaccttc tacaacacgc tcggcgtggc caagtggtgc atgagccggc acgtgcaccg
      301 cgcggggtac aagagcgtga tcaccggcga gggatcggac gagttgttcg gcggctatcc
      361 tgcgctgaaa cgcgacatgt tcctgcacgg gctcgaggac gagagcgacg agacgcgcgc
      421 ggcatacgag gcggcgctct acgagtcgaa ccggctgttt cgcggcgcga ttctcgccga
      481 gaaacaagag cggcacccgg cgctcgagga gctgtgcggc ttcacgccgt cgtggatcca
      541 gccctggctg gccacgctgc ccgtcgcccg gtcgttgttg tcgaacgccg cgcacgagga
      601 gctggccgac tacgacccgg tcgccgagat cgcgagcaag ctcgaacccg gcgcgctccg
      661 cggccggcac gcgctggaca aggcgcagta cacctggtcc aagacgatgc tagagggaca
      721 gatcctcaac tggggcggcg accgtgtcga catggccaac tcgatggaat cgcgcccggc
      781 gtttctcgat cacaacgtgg cgcaggccgc ggtggacgtc ccgccccggc tgcgaatccg
      841 cggcacgacg gagaaatggg tactgcgcga ggccgtgcgc ggcgtgctgc ccgacgcgtt
      901 gtacacccgt gagaagttcg cgttcatggc gccaccggcc cacaccgatc ggcgcaaacg
      961 ccgccgcgtc gaacgcctga tcgaaggcta cctcaacgcg gcctcggtcg aggaggcagg
     1021 tctgctcgac ccggcgcgcg tcgcggcgtt cgtcgacggc tggcgtcgcg acggcgatcc
     1081 ggtgtcgctg atccgcaagg acgggctgct caaccacctg ctttgcctgc aggttctcca
     1141 tcgccagttc atcgccgccg acgcgccggt agcgctcgtc ggctgagcca gccgccgccc
     1201 gcgccggccg tggaggatcg cgttgaagtc gagcgacgaa accgacccga agttcctcaa
     1261 catcgatccg ctgcgcaaga gctacaccga gcggatcgtc gagaacgtcg agcagatcga
     1321 ggcgctgcgc gcgcgcaatg tcgcgctgtt gtgcatcgat ctgcagtatc tcgacgcggc
     1381 gccgggccac ggcgtcttcc gcgaagccga gaaatcgggc gttccgctcg aggcccaggc
     1441 cttctacttc tcgacgttgg agcacacggt gctgcccaac gtgcgtcggc tgcaggacgt
     1501 gttccgcgac caccggctgg aagtgatcca cgttcgaatc tgctcgctga cgcgcgacgg
     1561 gcgggaccgc tcgacgggcc accggcgtct cgggctgttg gcggcccccg gctcgcgcga
     1621 ggccgacttc atcgagcgcg tcgcgccgct cggcgacgag atcgtgatca acaagacggc
     1681 cagcggcgtg ttcaactcga ccaacatcga gtacgtgctg cggaacatcg gtgtcgactc
     1741 gctgttcgtt accggtgtct acacggacga gtgcatctcg acgacggtgc gcgatgccaa
     1801 cgaccgcggg ttcttcgtca ccgtgatcga ggacggctgc gccacggtga cgcccgaccg
     1861 ccacgacttc actatcgcga ccctgcgcga ccgttacacg cgtgtgctga cgaccgaacg
     1921 cgcgatcgac gagatctcgc gccacgtcga cgccgacgcg agctgacccg ccggtcgggg
     1981 tggccacccc gcgctgcgga gcctagattc gccgcgatgg gatcgagcgc cgacctccga
     2041 tccgcgatcg attggccgcg ggccgcgctt ttggtgttgc tcggttggtg gacggcgcgg
     2101 atcgtcctcg gcacggactg gctgtttctc gatctcgtca atctcgcctt ccacgaagcg
     2161 gggcacctgt tcttcggctt cgccggctcg accgtacact acctcggagg cacgctcggc
     2221 cagctgatcg tgccgggcgg cctggtcgcc tacttcctgg tccggcggcg ccagccgttc
     2281 gccgccgcct tctgcctgtg gtgggcgggc gagagcctgg ccaacgtctc ggtctacatg
     2341 gccgacgcgc gcgagctggc gttgccgctg gtcgggggcg gcgatcacga ctggaacgag
     2401 ctgttcttcc gttgcggcat gctgaccgag ccggccgtcg cccggatctc gggcctgacc
     2461 cgtctcgccg ccgtcggcgt gatgctgtcg gcgctcggct ggggatccgc gctggcgttc
     2521 aaccgctgag cggccgcaac gccgcgccgt ccgtagctac tcgtccgcgg ccggcggcag
     2581 accgccccac ggccggaagg tggccccgcg ggacgcgcgt acgccccaca accgcaacgg
     2641 gatcgcgcgg tgtgaccatt ccggcctgcc gatccgcctc acccggcgga gcagcgacag
     2701 gttgaccagc gcacgcagca ccgtcgacgc cgcgatggcg gcgaggaagc cgacaccgag
     2761 gtagcgaccg gcgaaggtca cggtcaggcc gccgacgacc gagccgagga tcatcgcgct
     2821 gccgtcgacc aggttgaaga acgcgaccgt gtcctcgcgg ccgcgtgtgg tctgcagcag
     2881 ggccgaggcc tgggccagct cgaacccgcc ccacgccagc ccggcgtaga actgcgccag
     2941 ggcgatccac cagaactcgc cggagatcag ccacagcgac gagacgggca ggacgaacca
     3001 gccgatgccg cgcagcagcg ggcccggtcc gatacgatcg gccagcgtcc cccagcgccg
     3061 catcgtcatc gacttgacga tcgccggcac ggcgacgagc agaccgacct gaccgtaact
     3121 gaagcgcagc tcgcgcagca tgtagggcag gaaaaaaggc gcggcgaccc acgccgcgaa
     3181 gtggaaggcc cagaggtaga tcgcgagccg gcggaacttg cgcgacccgc gcaacaccgt
     3241 cccgatggcg cggagcgggg tgccgcgccg tggccggccg cgctcgggaa cggcgagcaa
     3301 gaatccgctg ccgatcgccc ggaacagacc ggccagaccg aagaccacca cgaagccgag
     3361 cccgacgcgt ccctcccgct cggccagcga cagaatcgct ccgccaccga ggatcgccga
     3421 gacgacgccg acctgctgca ccgcgccgcg cagtccgaag aaccaaccgt tgcgctcgcg
     3481 cggcacgaca cgcgagacga gcccggtcca cgaagggacc gacagcccgg cggcgaacca
     3541 cgacaggacg gcgccgccga agaccagcgg gatggcgaac ggcgcccgca gcagtgccag
     3601 gccgccgagc agcgccagtg ccagcgcctg cagcaaccag ctccgcgcga tgaaggcgcg
     3661 cgctccgtgc accccgccgg ccattcgggc ggcgaccacc tgggacagcg cggtcgccgc
     3721 cgtcggcagc gccccgacca ggccgagcac cagcccgccg gcaccgagcc agaccatcgc
     3781 cgcgccgagg taggtctcgc cgagcccctg cgccagcgac tggaagaacc cctcgacggc
     3841 gagtggcttg gcgcccttcg gtccagccga cgtggaagcc ccgaccggcg agccggtcct
     3901 gatctcggtg tcgatggtcc ccgtccgtcc cggcggtcgt gcgacctggg tcgcgttcgc
     3961 ctcatccgga atctggtcga ccggcgcgcc ggttgtcaac cgcgtgatcc ggccgacgcg
     4021 gcgcgaagcc gcggggtcag tccgaattgc cgatcgacgc gacgcggacg acgcggttgc
     4081 cgatgaccgt gccggtcacg cggacgcgct tgcgcagatg ctcgaacagc acacggccgt
     4141 cgaagcgtgg gtcgtcgatc aggtactcgc gctcgtcgag cgtgttgagg gcgtaggctg
     4201 ccacctgtcc cgccgccttc cacctccgcg cgatcagcac gccctcgagg gtcacctcgc
     4261 gctcgcgcgg ctgggtcgag ttggtgtccg aggtcggcat gacgcggtct ctttcgtgcg
     4321 aacgcgacgg cgatcacccc ggcggagagc acgcgccgtg ccagcgcgtc ccgggggcga
     4381 gcttcgagcg aggatccgtc agtcgtccgt cggtttcgga accggggttt ccgctttcgt
     4441 ctcgcgcgtc ggaacgatgc ggtccgactt gaaccactcc ttcttgatgc cgtagcgagt
     4501 cagcagcttg tgcagctgtc gcgttgtgac cccggcggcc gtcgccgagg cgtcgatgcg
     4561 accgtggaag cgtgccaaca gctcgctgag gtagcgttgc tcggcgatct cgaccgcggc
     4621 ccgtcgcgcg tcggccaggc tacccctcgc cgcggcgtcg gccgccgcac cgcttccgcc
     4681 ggccgccatg atctcgggcg ggatcagctc ggcgccgatc gtctcgccga gggcgaggat
     4741 gtgcgctcgc tcgacgacgt tctccaactc gcgcacgttc cccggccagg cgtaggactg
     4801 gaacgcgtgc gatacgtcgg gggatagctg accgaccccc ttgggcaggt aggtgttgca
     4861 gttttcgatg aaccgctcga cgagcgcggg gatgtcctcg gggcgctcgc gcagcggcgg
     4921 gatcgcgatc gggaagacgt tcaatcggta gtacaggtcc gggcggaacg tcccctgtcg
     4981 ctgcatctgt ccgaggtcgg cgttggtcgc cgcgatcaag cgcacatcga cctcgacctc
     5041 ggtctcccca ccgacgcgta ccaggcgccg ttgttgcagc acgtcgagca gcttgatctg
     5101 ggccgacgac gagatcgtgc cgacctcgtc gagaaacagc gtcccgccgg cggcgccctc
     5161 gaacttaccg ggctttcggc ggatcgcccc ggtgaacgcc cccttttcgt gaccgaacag
     5221 ctcgctctcg agcagcgtgt cgggcatcgc gccgcaatgc accgcgacga acggccccgc
     5281 cgcgcggttg ctcagctcgt ggatcagccg cgccagcact cccttgccgg tgccggtctc
     5341 gccggtcagc agtaccgtcg tgcgcgtcgg ggcgacggca cggatcttgt cgagcaccgg
     5401 ccgcatcgcc cggctgacgg tctcgaccgc ggtgacccgc gggtcctggg cacccccgcc
     5461 cggcgcgaac acgcgtcgct tcatcgcacg acgcctccag ccatcccgtt cattcccgca
     5521 gacagacacc cagaccgggg tcttccttgg cgatcaccgt accatgctcc agtcggaccg
     5581 acccggcgga cggatcgtcg atcagatcga ggtgcccgtc gagatcggcg tacgccacgt
     5641 tgggccgcgc cagcgcgaac tgcaacgccg cagcgatgcc gagcgcggcc tcgtccatgc
     5701 acccgaccat cacctcgagc ctggcggcgc gcgctaccgc gttgatctgc aacgcctcgg
     5761 cgattccgcc cgccttcatc agcttgacgt tgaccatgtc ggccagatcg cgccgcgcca
     5821 gccggaaggc gtcgcgcagg gtcatcaggc tctcgtccgc catcatcggc agcgccgcct
     5881 tgctgctgac ccggccgagc tcgtggatct cgtcacgcgg cgtcggctgc tcgagcagct
     5941 cgagcgcggc gctgcgcgtg gcgtcgacga accgcagcga ctggtcgacc gtgtaaccct
     6001 ggttggcgtc gaagcgcagc tcgatctcgg gcccgaccgc gcgtcgcacg gcgagcactc
     6061 gcgcgatgtc ctgttcgacg tcggtgccac ccttcatctt caggcagctg aaaccccgcg
     6121 ccaggtagtc gcgcgcggcg gcgaccgtct cgtcctgcgg caggattccg atcgtcacgc
     6181 tggtgcggat acgccgccgg tacccgccga gcaggcgcca gaccggcagc ccggcgacct
     6241 tccccaggat gtcgaacagc gccatgtcga tcgcggcgcg cgccgacggg aagtcggcca
     6301 ggctcttctt cgcccgcatc atcagcatcg cgtgccgcag cgggtccgag ccggtcagct
     6361 cggcgcgcgc gattccgttc agcgcggcca gcacgccggc cggttgctcg ccggtgacct
     6421 gggggtcggg ggcagcgcaa ccgtagccca ccgtcccgcg gttggtctcg acgcgcaaga
     6481 agacgttcgt cgcctggctg acgctctcgt aggcgatcga gtacggctga gccaaccgca
     6541 tcgtcacctg ccacgcctcg agcgcgctga ttctcatcgt cgtctcagta gcgccgctga
     6601 tagatttcgt cgagactcaa tcgcttgagg tcctcgatca actcgaccag tcgtcgcgtc
     6661 atcagttccc acagcttcac gcctttttcc gccgtcgcct tggtcggatc gcccatcacg
     6721 ccgctcggcg agatcttgtc ggtgtaggcg taccagccga cgct
//
LOCUS       DASPGN010000125         5773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1661493, whole genome shotgun sequence.
ACCESSION   DASPGN010000125 DASPGN010000000
VERSION     DASPGN010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5773
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1661493"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VD788_03460"
     CDS             <1..285
                     /locus_tag="VD788_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB family protein"
                     /protein_id="HXV75350.1"
                     /translation="LAAAHPATIVAAFVAAPLTSLSPVIGAGHVTAFVQAYFRPPRVR
                     DFEKVSEEIPRFGRWWRNRLLRVFLAFILPTLGSALGTWLGGARILRDLF"
     gene            complement(282..1574)
                     /locus_tag="VD788_03465"
     CDS             complement(282..1574)
                     /locus_tag="VD788_03465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXV75351.1"
                     /translation="MKNHLRESRGELGSFIVDDHATILGFDLGMELLTGWPAIEVVGR
                     AKNHCGGIALDGEGPDRIPPGTSALYDGAVPVDCNLQSIQLKLRCRDGRLLETEAVSE
                     RLDGAVDRARVTILRVLSLSAVDGIRAGAELHDELTLLPNGEAFRATLDGDLLAAQRA
                     SRPLALVLADVDRLRRVNDRWGREAGDAVLHKLAGILRVKADDENRIFRIGEDEFAIL
                     LPGAGRGEARQQAAALRSTVERFRFLNDHDVRDESPITLSLGAASFPADADNATDLFE
                     RAAEALDEARSMGRNRVWCYLRRPRLPIEVPVFFDGAEASPVGYTRDLSPSGVFVMTA
                     TPIDIGMRCALSFPLPGLDGRVHVIGRIVRTVPPESATATDDERVPGMGIEFERFGGI
                     SDRRAIEAFLHQSRPQSSAIGSRPARLVARAARTGSAD"
     gene            complement(1727..2764)
                     /locus_tag="VD788_03470"
     CDS             complement(1727..2764)
                     /locus_tag="VD788_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75352.1"
                     /translation="MNRSTRPRLALVALGAALAVGGTRDSTASEPVTAEPARPVFDDA
                     FWRLWGDGRAELAGYELIYDRYGEPRRGTAVTVFVTETFAGPPRVKHERASRPEGHTF
                     PVMKLNLMQDFPTGIYDYHLMTSTFVALGDSAFSRSGAPTKISFSSQEWCGQVYSQLL
                     FDAEEVRYQSHSYFDGEADAADVLDLPADGLAEDALLPWARGMTAPLLQPGESRAVRL
                     LRSLERSRLRHLPSEWLPATLHRSASPEQIRVPAGVFEVDRLDVRIEPPATQATYPPE
                     SRRAEPTTRSWTFLVERAPARRVVRWERSDGTTAELLGEARLPYWELNGAGLESEVER
                     LGLTPRPPRTP"
     gene            complement(2992..4053)
                     /locus_tag="VD788_03475"
     CDS             complement(2992..4053)
                     /locus_tag="VD788_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXV75353.1"
                     /translation="MMRLPGFRYRSPRSVEEAVRILAGEGPQASVIAGGTDLLPNMKR
                     RHQLPRTLVSLSRIDELRARRNGDGLVLGAGLTLTDVVEDARVQRAYPALWQAAAQIA
                     TPHLRNMGTLGGNLCLDTRCTYYNQNHEWRAAISYCMKCEGQTCWVAPASKRCLAVSS
                     TDTAPALVALDARVTLRSVDGERELAASELFHNDGIDYLNRRPGEILTAVRLGSRPNR
                     RSAYWKLRRRGSFDFPVLSVAAAATLDGDGTVADARIVLGAVASRPLFAERASEELVG
                     RPLDDDAIATAAAEAAKSAKPMDNTDFSLHWRKRVAAAFVGYALRELRGDDMRGPRRT
                     LTRWNPGAIASNLHPDGST"
     gene            complement(4050..>5773)
                     /locus_tag="VD788_03480"
     CDS             complement(4050..>5773)
                     /locus_tag="VD788_03480"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXV75354.1"
                     /translation="SDPFAHEFAACLLSMRTKRPVKFTLDREEVFYAHRGRHPVLMRF
                     RTGVKNDGTITGMHLQTLLDGGGYGSYGVASLFYTGALQTVTYRLPRYRFEGCRTFTN
                     KPPCGPKRGHGTPQPRFGQEIQLDKIAERLSIDPAELRLRIVEQPDTLTANYLKIGSI
                     GLAECIRRVVDASGWTDKHRKLPEGRGVGLACGSYLTGAGLPIYWNKLPHSGVQLQLD
                     RGGGVTVFCGCTEIGQGSDDMLALMVAEVLGIDPNDVRCVTGDTGITPVDLGSYSSRV
                     TLMMGNAAIQAALRARRLVAAAVAARLDTDPDALRFAEGKVVDPDGRSFSFREAVALA
                     EEEHGTLGTVGSYTPPRSAARFKGGGVGPSPAYSYTAAVVEVEVDRVTGWIHVPRVWV
                     AHDLGRVLNPVLARGQVEGSVYMGLGEALMEEQAFRRLPTKLSGALVHKFPSMLEYKS
                     PTSLDMPVVTTILVESPDPNGPFGAKEVGQGPLLPIMPAVANAVWDAVGVRIDECPIT
                     PEKIARALDDLERGGGGRVGPTRFPDVGYPEPLWVVPPDEGGDGRASNDPERKPAARM
                     GEDAVRS"
BASE COUNT          909 a         1998 c         1959 g          907 t
ORIGIN      
        1 ctggccgctg cgcacccggc gacgatcgtc gccgcgttcg tcgcggcccc gctgaccagc
       61 ctgtcaccgg tcatcggcgc cggtcacgtg acggcgttcg tccaggccta cttcagaccc
      121 ccgcgcgtgc gtgatttcga gaaggtctcg gaagagatcc cgcggttcgg ccggtggtgg
      181 cgcaatcggc tcctgcgcgt ttttctcgcg ttcatcctgc cgacgttggg cagcgctctc
      241 ggcacgtggc tcggcggcgc ccggatactg cgcgacctgt tctagtccgc tgagcctgtc
      301 cgcgcggctc gagccacgag ccgcgccggg cgcgacccga tcgcgctcga ctgtgggcgc
      361 gactggtgca ggaaggcctc gatcgcccgg cggtcggata tcccgccgaa acgttcgaac
      421 tcgatcccca ttccgggaac ccgctcgtcg tcggttgccg tcgcggactc gggcggcacg
      481 gtgcggacga tgcggccgat gacatggacc cgtccgtcga gccccggtag cggaaacgac
      541 aacgcacacc gcatgccgat gtcgatcggg gtcgccgtca tcacgaacac cccgctcggc
      601 gagaggtcgc gtgtgtagcc gacgggcgag gcctcggccc cgtcgaagaa gaccggaacc
      661 tcgatcggca gccgcgggcg tctgaggtag caccagacgc gattgcgtcc catcgaacgc
      721 gcttcgtcca acgcttcggc ggcgcgctcg aacaggtcgg tcgcgttgtc ggcatcggcg
      781 ggaaacgaag ccgcacccag gctgagcgtg atcggcgact cgtcgcggac gtcgtgatcg
      841 ttcaggaagc ggaaccgctc gacggtcgac cgcagcgcgg ccgcctgctg acgtgcctca
      901 cctctgcccg cgcccggcag caagatcgcg aactcgtcct cgccgatgcg gaagatgcga
      961 ttctcgtcgt cggccttgac gcgcaggata ccggccagct tgtgcaagac ggcatcgccc
     1021 gcctcgcggc cccagcgatc gttgacgcgc cgcaaccgat cgacgtcggc gagaaccagc
     1081 gccaggggtc gcgacgcgcg ctgcgccgcg agcaggtccc cgtccagcgt ggcgcgaaag
     1141 gcttcgccgt tggggagcag cgtcaactcg tcgtgcagct cggcgcccgc ccggattccg
     1201 tcgacggccg atagcgaaag cacgcggagg atcgtcaccc gcgcccgatc gaccgccccg
     1261 tcgagtcgct cggagactgc ctcggtctcg agcaggcgac cgtcgcggca gcgcagcttg
     1321 agctggatcg attggaggtt gcaatcgacc ggaaccgcgc catcgtacag cgcgctggtt
     1381 cccggcggaa tccggtcggg tccctcgccg tcgagcgcga ttcctccgca atgattcttg
     1441 gcacggccga cgacctcgat cgccggccaa ccggtcagca gctccatgcc gagatcgaag
     1501 ccgagaatcg tcgcgtgatc gtccacgatg aacgacccca gctcgccacg agattcgcgt
     1561 aggtggttct tcatccgctc atcctcctcg tgcggcggaa tctatgtctc catttcgtaa
     1621 tgaacaagtg tggagactac aaaaccgaat gtcgattcgg tcattgtcga tatgcgtcag
     1681 gagaaggatt acgaaacggg aatcgttggc cggcttcgcg acgcggctac ggcgtgcgcg
     1741 ggggtcgggg tgtcaatccc aatctctcga cctcggattc gagccccgcg ccgttgagct
     1801 cccaataggg gagacgcgct tctcccagca gctcggcggt cgtcccgtca ctgcgctccc
     1861 aacggaccac tcgtcgcgcc ggagcccgct cgacgaggaa ggtccaactc cgggtcgtcg
     1921 gctcggcgcg gcgagattcg ggcgggtagg tcgcctgggt cgccggcggc tcgatccgca
     1981 cgtccagccg atcgacctcg aaaacacccg ccggaacccg gatctgctcg ggactcgccg
     2041 accgatgcag cgttgcgggc agccactcgg aaggcaggtg tcggagccgc gatcgctcga
     2101 gcgagcgcag caggcgcacc gccctcgatt cgcccggctg gagcaaggga gcggtcatcc
     2161 cccgcgccca aggcaggagg gcgtcctcgg cgagaccgtc cgcgggcagg tcgagcacat
     2221 cggccgcgtc cgcttcgccg tcgaagtagc tgtgagattg gtagcgcact tcttcggcgt
     2281 cgaagagcag ctgggagtag acctgcccgc accactcctg ggacgagaac gagatcttgg
     2341 tcggtgcgcc agagcgcgag aaggcagagt cgccgagggc cacgaaggtg ctggtcatca
     2401 ggtggtaatc gtagatcccg gtcgggaagt cctgcatcaa gttcagcttc atcaccggga
     2461 aggtgtgacc ctccgggcgc gaagcccgct cgtgcttgac ccgcggcggt ccggcgaagg
     2521 tctccgtcac gaaaaccgtg acggccgttc cacggcgggg ttcgccgtag cggtcgtaga
     2581 tcagctcgta gcccgcgagc tcggccctgc cgtcgcccca cagtcgccag aaggcgtcat
     2641 cgaatacggg ccgagccggc tcggcggtca cgggctcgga agccgtggaa tcccgcgtgc
     2701 ccccgaccgc gagcgctgcc ccgagcgcga cgagggcgag ccttggccgt gtcgatcgat
     2761 tcaccgcgat ctccttcgcg gtcattctgc ccgagggccg aggttgtgcg cgaagacaaa
     2821 aaaggccccg gcggaggctg gagggggatc agctcgactc gccggggccc agtccttcac
     2881 aggaggaggt cggggtacgt ccaaacggaa agcaaatcga atgccagccg ttcgggccct
     2941 cgctccgggc cgttcggtgt cgccgatcgg tcccgcgtca cgcgacgcgc gtcaggttga
     3001 cccgtcgggg tgcagatttg acgcgatcgc gccgggattc caccgcgtca acgtgcggcg
     3061 cggcccacgc atgtcgtcgc cgcgtagctc ccgcagcgcg tagccgacga aggcggccgc
     3121 gacgcgtttc cgccagtgca gcgaaaagtc tgtgttgtcc atcggtttcg ccgactttgc
     3181 cgcctccgcc gcggcggtcg cgatcgcgtc gtcatcgagc ggccgcccga ccaactcctc
     3241 ggacgcgcgc tcggcgaaca gcgggcgcga ggcgacggcg ccgaggacga tccgcgcatc
     3301 cgccacggtc ccgtcgccgt cgagcgtcgc ggcggccgcg acggacagca ccgggaagtc
     3361 gaacgaaccg cgccgtcgca gcttccagta ggcgctgcgg cggttcggcc gcgatccgag
     3421 acgcaccgcc gtcaggatct caccggggcg gcggttcagg tagtcgattc cgtcgttgtg
     3481 gaacagctcg gacgcggcca gctcgcgctc gccgtcgacc gagcggagcg tgacccgcgc
     3541 gtcgagcgcc acgagcgccg gcgcggtatc ggtcgacgac acggccaggc atcgcttgct
     3601 ggcgggtgcg acccagcagg tctgaccctc gcacttcatg cagtacgaga tcgccgcccg
     3661 ccactcgtga ttctggttgt agtaggtgca tcgcgtgtcg agacagaggt tgccgccgag
     3721 cgttcccatg ttgcgcagat gcggcgtcgc gatctgggcc gccgcctgcc acaatgccgg
     3781 ataggcgcgc tgtacgcgag cgtcctcgac gacgtcggtc agcgtcagcc ccgcgcccag
     3841 cacgagcccg tcaccgtttc gcctggcacg cagctcgtcg atccgcgaga gcgacacgag
     3901 cgtgcgcggg agctggtggc gtcgtttcat gttcggcagc aggtcggtcc cgcccgcgat
     3961 caccgacgcc tgaggtccct cgccggccag gatgcggacc gcctcctcga ccgaacgcgg
     4021 cgaacggtag cggaaaccag gcagcctcat catgagcgca ccgcgtcctc acccatgcgc
     4081 gcggctggct tgcgctcggg atcgttcgac gcgcgcccgt cgccgccctc gtccggcgga
     4141 acgacccaca gcggctccgg gtacccgacg tccgggaagc gagtcggacc cacccgcccg
     4201 ccaccgccgc gttcgagatc gtccagtgcc cgcgcgatct tctcgggcgt gatcgggcac
     4261 tcgtcgatcc gcaccccgac cgcgtcccag acggcgttcg cgaccgcggg catgatcggc
     4321 agcagcggtc cctgcccgac ttccttcgct ccgaacggac cattgggatc gggcgactcg
     4381 acgaggatcg tcgtcaccac cggcatgtcg agtgaggtcg ggctcttgta ttcgagcatc
     4441 gacgggaact tgtggaccag cgccccggag agcttggtcg gaagacgccg gaacgcctgc
     4501 tcctccatca gtgcctcgcc cagacccatg tagacgctac cctcgacttg gccgcgcgcg
     4561 agcaccggat tcaacacgcg tccgaggtcg tgcgcgaccc agacgcgagg gacgtggatc
     4621 caacccgtga cacggtccac ctcgacctcg accaccgccg cggtgtagga atacgcgggt
     4681 gacggtccga cccctccgcc tttgaatcga gcggccgagc gcggcggcgt gtacgagccg
     4741 acggtaccga gcgtgccgtg ctcctcctcg gccaaggcaa ccgcttcgcg gaagctgaag
     4801 ctgcgaccgt cggggtcgac gaccttccct tcggcgaagc gcagggcatc gggatcggta
     4861 tcgagcctgg cggcgaccgc ggcggccacc aaccgacgcg cgcgcagcgc ggcctggatc
     4921 gcggcgttgc ccatcatcag ggttacccga ctggaatagg agccgagatc gacgggcgtg
     4981 atgccggtgt cgccggtgac gcagcggacg tcgttcgggt cgatgccgag cacctcggcg
     5041 accatcaggg ccagcatgtc gtccgacccc tgaccgatct ccgtgcatcc gcagaacacg
     5101 gtcacgccgc cgccgcggtc gagctgcagt tgaacgccgg aatgcggcag cttgttccag
     5161 tagatcggca gacccgcccc ggtgagatag gagccgcagg ccagaccgac cccgcgtccc
     5221 tcgggcagct tgcggtgctt gtccgtccac cccgatgcgt cgacgactcg acggatgcac
     5281 tcggcgagcc cgatgctgcc gatcttcagg tagttcgcgg tcagcgtgtc gggttgctcg
     5341 acgatccgga gcctgagctc ggccgggtcg atcgacaagc gctcggcgat cttgtcgagc
     5401 tggatctctt gaccgaatct gggttgcggc gttccgtgcc cccgcttcgg cccacatggc
     5461 ggcttgttgg tgaaggtgcg gcatccttcg aagcggtagc gcgggagtcg gtaggtcacc
     5521 gtctgcagtg ccccggtgta gaacaggctg gccacgccgt acgagccgta gccgccgccg
     5581 tcgagcagcg tctgcaggtg catgccggtg atcgtgccgt cgttcttcac cccggtgcgg
     5641 aagcgcatca gcaccggatg gcggccgcgg tgcgcgtaga agacctcctc gcggtcgagc
     5701 gtgaacttca ccggccgctt cgtgcgcatc gagaggaggc acgccgcgaa ctcgtgggcg
     5761 aacggatcgc tct
//
LOCUS       DASPGN010000126         4105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_372992, whole genome shotgun sequence.
ACCESSION   DASPGN010000126 DASPGN010000000
VERSION     DASPGN010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372992"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VD788_03485"
     CDS             <1..196
                     /locus_tag="VD788_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75355.1"
                     /translation="IVHNSLLFDHPERSVAWAPSVIRPKPPWFADHRDLVTLGRRVTR
                     FEAAPSWSALPALALAAFSG"
     gene            complement(209..1036)
                     /locus_tag="VD788_03490"
     CDS             complement(209..1036)
                     /locus_tag="VD788_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV75356.1"
                     /translation="MSELTGKRVLITGGAQGIGLEMGIQFAGAGAAVVLADLDEARLD
                     EARAAVEVVGGEVWTGTVDVTDPASIATLRERVAAEAGPVDVLVNNAGVVFGGPFADV
                     PLEQHFRTFQVNTLGVVAMTHAFLPDLVARPEAHLVNIASASGFIGLPHGSTYASSKW
                     AVIGFSESIRAELKLIGQDHVRVTIVCPSFIGTGMFAGASPPKTTSLLEPEFVARKVV
                     EAVEHDTLYVLEPWLVKLTPILRDLLPTRLFDKLGSALGASTSMTGWTGRDSDRDRR"
     gene            complement(1110..1799)
                     /gene="pgl"
                     /locus_tag="VD788_03495"
     CDS             complement(1110..1799)
                     /gene="pgl"
                     /locus_tag="VD788_03495"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXV75357.1"
                     /translation="MRLRLIADPDELAHTAADRIATAARACVARTGRFTFAASGGRTP
                     WQVFRRLAERDLPWHGFHLLQVDERVAPDGDPARNLTHLERSLLAHAPLPADNVHAMP
                     VGAVDLEHAAADYGRILDRLCGGRIDLVHLGLGDDGHTASLVPGDAVLAVTDRDVALT
                     RSSYAGHRRMTLTFPALARARSILWIVTGDGKAAALVGLLAGDRTIPAARVPREPATI
                     LADASAAALNR"
     gene            complement(1796..3442)
                     /gene="pgi"
                     /locus_tag="VD788_03500"
     CDS             complement(1796..3442)
                     /gene="pgi"
                     /locus_tag="VD788_03500"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HXV75358.1"
                     /translation="MKQDGTRPTERPEWRALERHYLEIRDRHLRQLFADDPRRGSRLS
                     CEAAGVYLDYSKNRLTDRTIELLIALAEAVSLPARIEAMFRGERINVTEDRAVLHVAL
                     RAPRGARIVVDGADVVPAVHEVLDRMAAFADAVRSQAWRGHSGQPIRDVVNIGIGGSD
                     LGPVMAYEALRHYSDRRIGFHFISNIDPTDFAEVVGRCDPERTLFIVCSKTFTTLETL
                     SNARAARRWLLDRLRDEAAVARHFVAVSTNEREVRDFGIDPANMFGFWDWVGGRYSYE
                     SAVGLSLMIAIGDERFRQMLAGFHQMDEHFRTAPLGRNLPALLGLIGVWYNDFFGAET
                     LAVLPYDQYLWRLPAYLQQLDMESNGKRIGLDGRPVELETGPIVWGQPGTNGQHAFYQ
                     LIHQGTKLIPCDFIGFARTLNPVADHHDLLMANYFAQTEALAFGKTAEEVAAEGVAPA
                     LVPHRTFPGNRPTNSILLDELTPAALGKLIALYEHKVFVQGTVWNINSFDQWGVELGK
                     VLAQRIVPELAAGDEQRLSHDDSTNELIRRFRERRGRAGR"
     gene            3520..>4105
                     /locus_tag="VD788_03505"
     CDS             3520..>4105
                     /locus_tag="VD788_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HXV75359.1"
                     /translation="MNEQSPPPPSPPVGDKPTATLPFEEPGRAFGAGLLETIKLFILD
                     PDQAFRRMPLRSDILRPLIYAVIVGWVGTAVAYLWGLMFQASILGMLGGLGDAEQVIP
                     PILLGVTAGVLGLVLAPVIILIQVFIYTVVIHLCLMLVGGERNGFATTLRVLCYSNTA
                     QLAQGIPFAGGLIATLWGIVLCVLGLMRSHDTTAG"
BASE COUNT          621 a         1424 c         1411 g          649 t
ORIGIN      
        1 catcgtccac aattcgctgc tgttcgatca ccccgagcgc tcggtcgcct gggccccgtc
       61 ggtgatccgc cccaagccgc cgtggttcgc cgaccatcgc gatctcgtca cgctcggacg
      121 acgggttacg cggttcgagg ccgcaccgag ctggtccgcg cttccggcgc tcgcgctggc
      181 agcgttcagc ggctgacgcg ccggggtctc agcgccgatc gcgatcggag tcgcgaccgg
      241 tccagccggt catgctggtg ctcgcgccga gcgccgaacc cagcttgtcg aacaggcggg
      301 tcggcagcag gtcacgcagg atcggggtca gcttgaccaa ccacggctcg agcacgtaca
      361 gcgtgtcgtg ctcgaccgcc tcgacgacct tgcgcgcgac gaactcgggc tcgagcaacg
      421 acgtggtctt cggcggggaa gcgccggcga acatgccggt accgatgaag ctcgggcaaa
      481 cgatcgtcac gcgcacgtgg tcctgaccga tcagcttcaa ctcggcacgt atcgactccg
      541 agaagccgat caccgcccac ttgctcgatg cgtaggtcga cccgtgaggc agaccgatga
      601 aaccggaggc gctggcaatg ttgaccaggt gcgcctcggg ccgcgccacc aggtcgggca
      661 ggaacgcgtg ggtcatcgcg accacgccga gcgtgttgac ttgaaacgtc cggaagtgct
      721 gttccagcgg gacgtcggcg aacggaccgc cgaagacgac gccggcgttg ttgaccagca
      781 cgtcgaccgg gccggcttcg gcggcgacgc gttcccgcaa cgtggcgatg ctcgccgggt
      841 cggtgacgtc gaccgtcccg gtccacacct ccccaccgac cacctcgacc gccgctcgcg
      901 cctcgtcaag ccgtgcttcg tcgagatcgg cgagcaccac ggcggcaccg gcgcctgcga
      961 actggatccc catctcgagc ccgatgccct gggccccgcc ggtgatcagc acccgtttgc
     1021 cggtcagctc gctcatcgca gagtcatggt agcagcgcgt cgggtggggt tgcggcacga
     1081 ccggcagcga ttggagagtg cgatggtcgt catcggttca gcgcggcggc actcgcgtcg
     1141 gcgagaatcg tcgccggctc gcgtggtacc cgcgcggccg gaatcgtgcg gtcgccggcg
     1201 agcaagccga ccagcgccgc cgcctttccg tcgccggtga cgatccacag gatcgaccgc
     1261 gcccgggcca gcgccgggaa ggtcagcgtc atccggcggt gaccggcgta ggacgaccgg
     1321 gtgagcgcca cgtcgcgatc ggtcacggcc agcacggcgt cgcccggcac gagcgacgcg
     1381 gtgtggccgt cgtcaccgag accgaggtgg acgagatcga tacgaccgcc gcacagccgg
     1441 tcgagaattc ggccgtagtc ggcggcggcg tgctcgaggt cgaccgctcc gaccggcatc
     1501 gcgtgcacgt tgtcggcggg tagcggggcg tgcgccagca gcgaacgttc gagatgggtc
     1561 aggtttcgcg cgggatcgcc gtcgggcgcg acgcgctcgt cgacctgcag caggtgaaaa
     1621 ccgtgccacg gaagatcgcg ctcggccaga cggcggaaga cctgccacgg tgtccgtccg
     1681 ccgctggcgg cgaaggtgaa gcggccggtg cgcgccacgc aggcgcgcgc ggcggtcgcg
     1741 atccggtcgg cggcggtgtg tgccagctcg tccggatcgg cgatcaatcg caatctcacc
     1801 gcccggctcg gccgcgtcgc tcgcggaacc ggcggatcag ctcgttggtc gagtcgtcgt
     1861 gggacaaccg ctgctcatcg ccggccgcga gctcggggac gatccgctgg gccaacacct
     1921 tgcccagctc gacgccccac tgatcgaacg agttgatgtt ccacaccgtg ccctggacga
     1981 acaccttgtg ctcgtagagc gcgatcagct tgcccagcgc cgccggggtc agctcgtcga
     2041 gcaggatgct gttggtcgga cggtttcccg ggaaggtgcg gtgcgggacc agcgccggcg
     2101 cgacaccctc ggccgccacc tcctccgccg tcttgccgaa ggccagcgcc tcggtctgcg
     2161 cgaagtagtt ggccatcagc aggtcgtggt ggtcggccac cgggttgagc gtccgcgcga
     2221 agccgatgaa gtcgcacggg atcagcttgg tcccctggtg gatcagctgg tagaaggcgt
     2281 gctggccgtt ggtacccggc tggccccaga cgatcgggcc ggtctccagc tcgactggcc
     2341 gcccgtcgag gccgatccgc ttgccgttgc tctccatgtc gagctgctgg agataggccg
     2401 gcaaccgcca caggtactgg tcgtacggca gcacggccag cgtctcggcg ccgaagaagt
     2461 cgttgtacca cacgccgatc agaccgagca gcgccggcag gttgcgcccg agcggagccg
     2521 tgcggaagtg ctcgtccatc tggtgaaacc cggcgagcat ctggcgaaag cgctcgtcgc
     2581 cgatcgcgat catcaggctc agcccgaccg ccgattcgta cgagtaacgc ccgccgaccc
     2641 aatcccaaaa gccgaacatg ttggccgggt cgatgccgaa gtcgcgcacc tcccgctcgt
     2701 tggtcgacac ggcgacgaag tgtctggcga cggccgcctc gtcccgcagc cggtcgagca
     2761 gccagcgccg cgccgcacgg gcgttggaca acgtctcgag cgtggtgaac gtcttcgagc
     2821 agacgatgaa cagcgttcgc tccgggtcgc atctgccgac gacctcggcg aagtcggtgg
     2881 ggtcgatgtt cgagatgaaa tggaagccga tgcgccggtc gctgtagtgc cgcagcgcct
     2941 cataggccat caccgggccg aggtccgagc cgccgatgcc gatgttgacc acgtcgcgga
     3001 tcggctgacc gctgtggccg cgccatgcct gcgagcgcac ggcgtcggcg aacgccgcca
     3061 tccggtcgag cacctcgtgc accgccggca cgacgtccgc gccgtcgacg acgatcctcg
     3121 cgccgcgcgg cgcgcgcagc gcgacgtgca gcacggcgcg gtcctcggtg acgttgatcc
     3181 gctcgccgcg gaacatcgct tcgattcgcg cgggcagcga gacggcttcg gccaacgcga
     3241 tcagcaattc gatcgtccga tcggtgagcc ggttcttcga gtagtcgaga tagacgccgg
     3301 ccgcctcgca gctcaatcgg ctgccgcgcc gcgggtcgtc ggcgaacagc tggcgcaggt
     3361 gccgatcgcg aatctcgagg tagtggcgct caagcgcccg ccactccggc cgctcggtcg
     3421 ggcgcgttcc gtcttgcttc atcccggtcc tccgcgggcg cggtcgccgt gactgtaaca
     3481 cggtcgtccc gggcgcgatc ggggtgataa catcgcgcca tgaacgaaca atcgcctccg
     3541 cctccgtctc cacccgtcgg cgacaaaccg accgcgacgc tgccgttcga ggagcccggc
     3601 cgcgcattcg gcgccggatt gctcgagacg atcaagctgt tcatcctcga tccggaccag
     3661 gcgtttcgcc ggatgccgct ccggagcgac atcctgcggc cgctgatcta cgccgtcatc
     3721 gtcggctggg tcggtacggc ggtggcctac ctctgggggc tgatgttcca ggccagcatc
     3781 ctcggaatgc tcggcggtct cggagacgcc gagcaggtca tcccgccgat cctgctcggg
     3841 gtgaccgccg gcgtgctcgg cctcgtcctc gcgccggtca tcatcctgat ccaggtcttc
     3901 atctacaccg tcgtgatcca cctgtgcctg atgctcgtcg gtggcgagcg caacggtttc
     3961 gccaccacgt tgcgcgtact gtgctacagc aacacggcgc agttggcgca aggtatcccg
     4021 ttcgccggcg ggctgatcgc gacgctgtgg ggcatcgtgc tgtgcgtcct cggtctgatg
     4081 cgctcgcacg acacgacggc cgggc
//
LOCUS       DASPGN010000127         6994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1544253, whole genome shotgun sequence.
ACCESSION   DASPGN010000127 DASPGN010000000
VERSION     DASPGN010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544253"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VD788_03510"
     CDS             complement(<1..1236)
                     /locus_tag="VD788_03510"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75360.1"
                     /translation="MKPRIALGLLASVLLLGVPAPPPAAAGPDRIEETRARGEVYALL
                     MRALMAQRRGEYKNAAADVRKAIEIRPEDPAVLIQGAELLERMGRMRDAEELARQALA
                     LDPDNIEALWFVADRAAALALGTTKPEAKDRDEALRLYARLQELGVDDPEVQRKIVSL
                     KLQAGDQAGALAAAEHLVRQRPGDRHAVGMLGQLLLDSGEPKQALRVLVRFIADHPDD
                     SALIRLAEELAQDLDAWDVVEETFAQNEGFEDRAVEAQRLRSQALLRLERIEPASRAL
                     EKVLMTDPGDRNVRYHLARTYRRLGRLGEAADLARGLAEEAAADRAAHLLLAEVLDDQ
                     GSVESALNAYNTVLRLFSADDSIEQAQQIREAVRRRMALLYLVNDQPAAAERTLSAME
                     LPDEAETLQIWSRLAIERED"
     gene            complement(1296..2675)
                     /locus_tag="VD788_03515"
     CDS             complement(1296..2675)
                     /locus_tag="VD788_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75361.1"
                     /translation="MAPFVDRPITTLAGGEHLARERLSDGLGARTIAGVYQRAALASG
                     RSAAEGERRPFRALAALYLDRATREPDDELARIRAASGADPAEETGWMLEPDPVARRR
                     ALAASIAREDRESRWMRDRIELRERAARDAGATSVVGLLDAAQPRASAAIAREFERDA
                     IEPLDEAISAAARQRRQRFGLDPDENAASETPAFLSMAALAGRLPATAIHGVVRRVGT
                     RFGEDPDSAGPHLAPRPAPGPRSFVGLTRDGLAVIVAGAVAGPGGLRDLLGSFGVAAR
                     AGRIADLRGTAAVRFADPAFAHASSTLWRRLPLCPRFRREAGLDADDWEIRELAFQEA
                     VAMRLAWAYLQTAVTGIDGSSPAFDRIVRRSCGAAATPAERRAVADRDPSWSAQLRGN
                     LLGLLLEQRLRLRYGRDWFSRNEARRFLVEMWEAEPEMTAEEMAGSLDLGTIEATPMV
                     DAFRDGVAG"
     gene            complement(2740..3933)
                     /locus_tag="VD788_03520"
     CDS             complement(2740..3933)
                     /locus_tag="VD788_03520"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXV75362.1"
                     /translation="MKTGRSLLLLASTLVMVFLLGGGLIVKVGAADSSYRQAVLFAEV
                     LSQVLDNYVDPIEAEGLLEGAYDGMLGGLDPNGAYLSRAEAEEWKSVAAAGSQPGADP
                     GLTVLKSGRALQVVAVLDGSPAAEAGIKVGDQIRAVGEVEVRDLSLRQARHRIEGDPG
                     SAVQIELFHPADGFRRESVELVRRPRDFRPYRLDVERGTAVLRLLDLGRIDLDALGAE
                     LDDVRSRPTERLLIDLRNSAGSQPRAAATVAALFTSPPPLRLRDRSGRLVETIETPAR
                     ETAWTGSIAVLVNGATAEGAEALALLLGERADVPIFGEKTFGLGAEPSLFELESGSAL
                     LLSSAQWETADGKSWHDRGIDPDEVIAGEGDDYAEVAADQLQRVLERMESGRPVETPA
                     EREAA"
     gene            complement(4050..5198)
                     /locus_tag="VD788_03525"
     CDS             complement(4050..5198)
                     /locus_tag="VD788_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HXV75363.1"
                     /translation="MRGRRPAAAFLLSLVAIGVVGSTIAQQSVGRRERTRAVRDEIDR
                     LQAELESLGRRESGLLGELERLGAELRLRDAETRAAEVETEEIRAAIDERNAALGRLE
                     AQQADRRGYLAFRLREIYKQGPDRTLRRAVGDVQLESYLAGLRYAAFLSSRDARVLAD
                     YRTDARRLTGEREALAAREQELAVALAELEESKRRLSEARDRRSARLDEVRQDRDSRL
                     VALDELEAAADELSRLADSFLPGADAPRLDVQKFRGLLDWPADGAIEAGFGAIVHPRF
                     KTRLPHPGVDIGAEAGSEIRSVFDGKVVFASWMRGYGLTTIVDHGGGLISVYAHAAGL
                     WVEPGEEVLRGGTLGMVGETGSLRGPYLYFELRENGRPVDPTEWLRRR"
     gene            complement(5198..>6994)
                     /locus_tag="VD788_03530"
     CDS             complement(5198..>6994)
                     /locus_tag="VD788_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXV75364.1"
                     /translation="LAFFVLALALSPLLLRRWTLRVVAALLVGAAAGAAGGVAAARWL
                     PIEVPDTINNRKGRTMWELAAEAGFDVVVVRVPATFPAADIGTGRMISGLGVPDIRGR
                     VGTPAYYTSDPNFEPDDDFSLEPIKLAARRGTLDTRIVGPPNKAFYDFVVSREAEVAP
                     PDRRRAVRREARRRLDEAGVPSRLDLPLMLEASDTSLIVRAAGQTATLAVGQWSEWFE
                     LPFRINPLIDAIDPTIGIARFKLLALEPHLELYLSPINFHPDCHPVAFAWPPDYSDQI
                     RRRFGLYKTIGWALDTWSAPSGVGAEELFLEDMDFTVSKYAEIMDGLLADRDVDLYIQ
                     IFYFTDRIAHLFWRFIDPGHPLYDPAQAARYAPRILEAYRRMDELVGRARERAGDDAV
                     FIVCSDHGFSSFRRGINYNNWLVREGWMTLDDSAGPAPSLSDVDWKRTKAYALGLGSI
                     YVNLIGREREGVVLPGPEYDEVIRGIRQGLEQLVDPLTGERPITRVSTRDELYGDYDP
                     DLVPDLRVNNALNYRVSWGTTLGGFGQDVFEDNTNKWSGDHASSDPRVVPGILLANRP
                     IRHAPRMIDLMPTVLELLGVEPPDAVEGRSVL"
BASE COUNT         1052 a         2566 c         2371 g         1005 t
ORIGIN      
        1 atcctcgcgc tcgatcgcca agcgcgacca gatctgcagc gtctccgcct cgtcgggcag
       61 ctccatcgcc gacagcgtcc gttcggccgc cgccggctgg tcgttgacga ggtacagcag
      121 tgccatccgg cggcgaaccg cttcacggat ctgctgcgcc tgttcgatcg agtcgtcggc
      181 ggaaaacaga cggagcacgg tgttgtaggc gttgagggcg ctctcgacgc tgccctggtc
      241 gtcgagcacc tcggccaaca gcaggtgcgc cgcgcgatcc gcggccgcct cttcggccag
      301 gccgcgcgcg aggtccgcgg cctcgcccag gcggccgagc cggcggtagg tgcgggccag
      361 gtggtagcgc acgttgcggt caccgggatc ggtcatcagc accttttcca gcgcacgact
      421 ggccggttcg atccgctcca gccggagcag cgcctgactg cgcagccgct gcgcctcgac
      481 ggcgcggtcc tcgaacccct cgttctgggc gaacgtctcc tcgaccacgt cccaggcgtc
      541 gaggtcctgg gccagctcct cggccagccg gatcaacgcg gaatcgtcgg ggtggtcggc
      601 gatgaagcgg acgagcacgc gcagcgcctg cttcggctct ccgctgtcga gcagcagctg
      661 tccgagcatc ccgaccgcat gccggtcccc cgggcgctgg cggaccaggt gctccgcggc
      721 ggccaacgcg ccggcctgat cgcccgcctg cagcttcaaa ctgacgatct tgcgttggac
      781 ctcggggtcg tcgacgccca gctcttgcag acgagcgtag agcctcagcg cctcgtcgcg
      841 gtccttggcc tccggcttcg tcgtaccgag cgcgagcgcc gccgcgcggt cggcgacgaa
      901 ccacagcgcc tcgatgttgt ccggatcgag cgccagcgcc tgccgtgcca gctcctcggc
      961 gtcacgcatt cgccccatcc gttcgagcag ctccgcaccc tggatcagca cagcggggtc
     1021 ttcgggtcga atctcgatcg ctttgcgcac gtcggccgcc gcgttcttgt actcgccgcg
     1081 gcgctgggcc atcagcgcgc gcatcagcag cgcgtagacc tcaccgcgag cgcgcgtctc
     1141 ctcgatgcga tccggcccgg ctgccgcggg cggtggggcg ggaacgccga gcagcaggac
     1201 cgaggccaac aagcccaaag cgatccgcgg tttcatcggc atctcaggtc actcgctttc
     1261 actaccgctg ccggtcgagc ccggcggtcg ccgggtcatc cggcgacccc gtcgcgaaat
     1321 gcatcgacca tcggggtcgc ttctattgta ccgaggtcga gcgaaccggc catttcctcg
     1381 gctgtcatct cgggctcggc ctcccacatc tcgaccagga agcggcgggc ctcgttccgc
     1441 gagaaccagt cgcgaccgta ccgcaagcgc agccgttgct cgagcaacag cccgaggagg
     1501 ttgccgcgta gctgcgccga ccacgacgga tcgcggtcgg cgaccgcgcg gcgctccgcg
     1561 ggcgtcgccg cggcaccaca gctccgccgg acgatgcggt cgaatgcggg actcgagccg
     1621 tcgataccgg tcactgccgt ctgaaggtac gcccaggcca ggcgcatcgc taccgcctcc
     1681 tggaacgcga gctcgcgaat ctcccagtcg tccgcgtcga gacccgcctc acgacgaaag
     1741 cgcgggcaca atggcagtct tcgccacaac gtcgagctgg cgtgggcgaa tgccgggtcg
     1801 gcgaaccgga ccgccgccgt gccgcgtaag tcggcgatgc gaccggcgcg cgccgccact
     1861 ccgaacgagc cgagcaggtc ccgcaggccg cccgggcctg cgacggcacc cgccacgatc
     1921 acggccagcc cgtcgcgcgt caggccgacg aaggacctcg gcccgggcgc cggtcgcggc
     1981 gccaggtgcg gtccggcgga atcgggatct tcgccgaacc gcgtcccgac gcgtcgcacg
     2041 accccgtgga tcgccgtcgc cggcaagcgc ccggccagcg ccgccatcga caggaacgcc
     2101 ggtgtctcgg acgcagcgtt ctcgtccgga tcgagaccga accgctggcg acgctggcgc
     2161 gccgcggccg agatcgcctc gtccaacggc tcgatggcgt cgcgctcgaa ctcgcgggcg
     2221 atcgcggcgg aggcgcgagg ctgcgcggcg tcgagcaacc cgacgaccga cgtggccccc
     2281 gcgtcgcgcg ctgccctctc acgcagctcg atgcgatcgc gcatccaccg gctctcgcga
     2341 tcttcccgag caatcgacgc ggcgagcgcc cgacgtcgcg cgaccgggtc gggctcgagc
     2401 atccacccgg tctcctcggc tggatccgcc ccgctcgccg cccggattcg cgcgagttcg
     2461 tcgtccggct cgcgcgttgc gcgatcgagg tagagcgcgg ccagcgcgcg gaacggccgt
     2521 cgctccccct ccgcggccga tcgcccgctc gcgagcgccg cgcgctgata gacgcccgcg
     2581 atcgtccgcg cgcccaaacc gtcggagagc cgctcgcgcg cgaggtgctc cccgccggcg
     2641 agggtcgtga tcgggcgatc gacgaacgga gccacggcgc actcgtccgc ctcgccgacg
     2701 acccgatccg catcgacaat gggcgattcg atggacgggt caggcagctt cgcgttccgc
     2761 cggggtctcg acgggacgac ccgattccat ccgctcgagg acgcgctgca gctggtcggc
     2821 ggcgacctcg gcgtagtcgt cgccctcccc cgcaatcacc tcgtcgggat cgatcccccg
     2881 gtcgtgccag ctcttgccgt ccgcggtctc ccactgcgcc gacgacagca gcagcgcgga
     2941 gccgctctcg agctcgaaca gcgacggctc cgcgcccagc ccgaaggtct tctcgccgaa
     3001 gatcgggacg tcggcccgct cgccgagcag gagcgccagt gcctcggcac cctcggccgt
     3061 tgccccgttg accagcaccg cgatcgaacc ggtccaggca gtctcccgcg cgggtgtttc
     3121 gatcgtctcg accagtcggc cggagcggtc gcgcagtcgc agcggcgggg gcgaggtgaa
     3181 cagcgcggcg accgtcgccg ccgcccgcgg ttgcgatccg gccgagttgc gcagatcgat
     3241 cagcagtcgc tcggtggggc gcgaccggac gtcgtcgagc tcggcgccga gcgcgtcgag
     3301 gtcgatccgg ccgaggtcga gcagccgaag cacggccgtt ccgcgttcga cgtcgagccg
     3361 ataggggcgg aagtcgcgag gacggcggac gagctcgacg gactcccgtc ggaatccgtc
     3421 cgcgggatgg aacaactcga tctggaccgc gctaccgggg tcgccctcga tgcggtgtcg
     3481 ggcctgtcgc agggagaggt cgcgcacctc gacctcgccg accgcgcgga tctggtcccc
     3541 gaccttgatg cccgcctcgg cggccggcga tccgtcgagc accgccacga cctgcagcgc
     3601 gcggccgctc ttcagcacgg tgagaccggg atcggcgccc ggctgcgaac cggccgcggc
     3661 gacactcttc cactcctcgg cctccgctcg ggacaggtag gctccgttgg ggtcgagccc
     3721 accgagcatg ccgtcgtagg ctccctcgag caagccctcg gcttcgatcg ggtcgacgta
     3781 gttgtcgagc acctgcgaca ggacttcggc gaacagcacc gcctgacggt acgagctgtc
     3841 ggccgcgccg accttgacga tcagcccgcc ccccaagagg aagaccatga cgagcgtcga
     3901 agcgaggagc agcagagagc gtcctgtttt catcgtgtcg tctctcacgc gccagcgggc
     3961 ggaaacggcc ccaccccagc aagctacccc gcctcccgat gcttggcaac ccggaatccg
     4021 gcagaatctc ggcgacggcc gacggcgcgc tagcgacgcc tcagccattc cgtcgggtcg
     4081 accggccggc cgttctcccg cagctcgaag taaagatacg ggccccgcag cgaccctgtt
     4141 tcgccgacca tgccgagcgt gccgccgcgc agcacttctt cgccgggctc gacccacaat
     4201 ccggcggcgt gggcgtagac cgagatcaac ccgccgccgt ggtcgacgat cgtcgtcaag
     4261 ccgtagcccc gcatccacga cgcgaacacg accttgccgt cgaacacgct gcgaatctcg
     4321 ctacccgctt ccgcgccgat atcgaccccg ggatgcggga gccgcgtctt gaaccggggg
     4381 tgcacgatcg cgccgaaccc cgcttcgatc gcgccgtcgg cgggccagtc gagcagacca
     4441 cggaacttct gcacgtcgag ccgcggagcg tcggcacccg gcagaaacga atcggcgagg
     4501 cgcgacagct cgtcggccgc cgcctcgagc tcgtcgagcg cgacgaggcg cgaatcgcgg
     4561 tcttgcctca cctcgtcgag ccgggcggag cgccggtcgc gggcttcgga caagcgtcgc
     4621 ttcgactcct cgagctcggc gagcgcgacc gccagctcct gctcccgcgc ggcgagcgct
     4681 tcccgctcgc cggtcagacg gcgtgcgtcg gtacggtagt cggcgagcac acgcgcgtcg
     4741 cgcgagctga gaaacgccgc gtagcgcagc cctgccaggt acgactcgag ctggacgtca
     4801 ccgaccgccc ggcgcagcgt ccggtccggc ccttgcttgt agatctcacg caggcggaag
     4861 gcgaggtagc cacgccggtc ggcctgctgc gcttcgagcc gcccgagcgc ggcgttgcgc
     4921 tcgtcgatcg cggcacggat ttcctcggtc tcgacctccg ccgcccgcgt ctcggcgtcg
     4981 cgcagacgca gttcggcacc gagacgctcg agctcgccga gcagccccga ttcccggcgg
     5041 cccaacgact cgagctcggc ttgcagccgg tcgatctcgt cacgcaccgc ccgcgtccgt
     5101 tcgcgccgac cgacgctttg ctgcgcgatc gtcgagccga caaccccgat cgccacgagc
     5161 gacaggagga acgccgccgc cgggcgccgg ccgcgcatca caggaccgag cgcccttcga
     5221 ccgcatccgg cggctcgacg cccagcagtt ccagtacggt cggcatcaga tcgatcatcc
     5281 gcggcgcgtg gcggatcggc cggttcgcca gaagaatccc gggtacgaca cgcggatcgc
     5341 tcgaggcgtg gtccccggac cacttgttgg tgttgtcctc gaacacgtcc tggccgaatc
     5401 cgccgagcgt cgtaccccac gaaacgcggt agttcagcgc gttgttcacc cgtaggtccg
     5461 gcaccaggtc cgggtcgtaa tcgccgtaca gctcgtcgcg cgtcgacacc cgagtgatcg
     5521 gtcgttcgcc ggtcagcggg tcgacgagct gttcgagccc ctggcgaatc ccacgtatga
     5581 cctcgtcgta ttccgggcca gggagcacca cgccttcgcg ctcgcgcccg atcagattga
     5641 cgtagatgct gccgagaccg agcgcgtagg ccttggtccg cttccagtcc acgtcggaca
     5701 gcgagggcgc cgggccggcg gagtcgtcga gcgtcatcca cccttcacgg acgagccagt
     5761 tgttgtagtt gatcccgcgg cggaacgagg agaagccgtg gtccgagcag acgatgaaca
     5821 cggcgtcgtc gccggctcgc tcgcgcgcgc ggccgaccaa ctcgtccatt cgcctgtacg
     5881 cttcgaggat ccgcggggcg tagcgtgcgg cctgagccgg gtcgtacaac ggatggccgg
     5941 ggtcgataaa gcgccaaaac aggtgtgcga tgcggtcggt gaagtagaaa atttggatgt
     6001 acagatcgac gtcgcgatcg gcgagcaggc cgtccatgat ctcggcgtac ttgctcaccg
     6061 tgaagtccat gtcctcgaga aacagctcct cggcgccgac gccggatggc gcggaccagg
     6121 tgtccaacgc ccagccgatc gtcttgtaca gaccgaaacg gcgccggatc tgatccgagt
     6181 agtccggcgg ccaggcgaac gccaccgggt ggcaatcggg gtggaagttg atcggggaca
     6241 ggtagagctc gagatgcggc tcgagcgcga gcagtttgaa tcgcgcgatg ccgatcgtcg
     6301 gatcgatcgc atcgatcaat gggttgatcc gaaacgggag ctcgaaccac tcgctccact
     6361 gcccgaccgc gagcgtcgcc gtctgtccgg ccgcgcgcac gatcaggctg gtgtcgctcg
     6421 cctcgagcat cagcggtagg tcgagtcgcg acggcacgcc ggcctcgtcc agcctccgtc
     6481 gcgcctcgcg ccggaccgcg cggcggcggt cgggcggggc gacctccgcc tcgcgcgaga
     6541 cgacgaagtc gtagaacgcc ttgttcggcg gaccgacgat gcgggtgtcg agcgtcccgc
     6601 gcctggccgc cagcttgatc ggctcgaggc tgaagtcgtc gtccggctcg aaattcgggt
     6661 ccgaggtgta gtacgccggc gtgccgaccc gcccacggat gtcgggcacg ccgagaccgg
     6721 agatcatccg cccggtcccg atgtcggccg cggggaaggt cgccggcacg cggacgacga
     6781 cgacgtcgaa cccggcctcg gcggccaact cccacatcgt gcgtcccttg cggttgttga
     6841 tcgtgtcggg tacctcgatc ggcagccagc gcgccgcggc gacgccgccg gcggcacccg
     6901 ccgcagcgcc gaccaacagc gccgcgacga cccgtagcgt ccagcggcgg agcagcagcg
     6961 gcgagagcgc gagcgcgagc acgaagaaag cgag
//
LOCUS       DASPGN010000128         2747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1544286, whole genome shotgun sequence.
ACCESSION   DASPGN010000128 DASPGN010000000
VERSION     DASPGN010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1544286"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..685)
                     /locus_tag="VD788_03535"
     CDS             complement(95..685)
                     /locus_tag="VD788_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXV75365.1"
                     /translation="MRNDQRGFSLVELLVSVAVLVMALGGLASLLIQNARINKAQRMT
                     AEVQANARNTLSMVVQALRSAGWDPTNMGVPTVNLDPDTSDDVSEIEVFANLNEDPDT
                     DDAGEQILIRHLGSTREVVWRESSDTTQPFIVLSSNISNDADGDGTIEPMFVPDANPP
                     TRVTVQITAQSPAPDPTSGEFIRYTVSSDVVLRKAL"
     gene            complement(696..1118)
                     /locus_tag="VD788_03540"
     CDS             complement(696..1118)
                     /locus_tag="VD788_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXV75366.1"
                     /translation="MMGTSTTSTERVRVERGFSLVEVLVALFLIALGVLAAAPMFVYA
                     MQGNATGADFGSVGAIAVERMELLRASSFVSLAAGGSLTVSQSGYSDLSDPDFIVRWR
                     ITDDAAPPSVKTITVRAIAVRQVVGQRKEITLTTLRGR"
     gene            complement(1090..1791)
                     /locus_tag="VD788_03545"
     CDS             complement(1090..1791)
                     /locus_tag="VD788_03545"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXV75367.1"
                     /translation="MKNQKGMTLVELTLVMSVSTVAVVASATYALPWFSREKARGAVY
                     DVQSYMQLARIEAVSRNHQCRFVLDTSTRTLQVFDGNGTPADSSDDVVLYETVLTDQV
                     QFARPDVGVPVSLAQIGTTDSYEVVFTADGSVASGTGQVALYGGEEYNRVTVLGAGGI
                     TVDRWIGSGWTNGIYVTEVASSGGSGGSGGSGGSGGGEEGTVPIGELPPIDDVTIDDP
                     QGGGGTDDGYVYDIY"
     gene            complement(1992..>2747)
                     /gene="hemG"
                     /locus_tag="VD788_03550"
     CDS             complement(1992..>2747)
                     /gene="hemG"
                     /locus_tag="VD788_03550"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HXV75368.1"
                     /translation="TSFHDGLQELTDALAAALGERLRLGSPVVRLSDMGSRGFRILLP
                     EGAPIECDAVVLATPAWSAAPVVESMDPELHRLLGAIPSASVAVVHTGFRTLALGDQP
                     TGFGFLVPRGQGPRILGTLWISQIFDGRAPDGASLMTTMIGGAHDPAAVELDDRQLLE
                     LVHQDLKRTMAVMIRPYFSRIVRWSRGIPQYELGHPARLERIGERLAAHAGLFLCGNS
                     YRGISVNSCVEEAPGVAESVLEFLARRPGVSFD"
BASE COUNT          448 a          931 c          920 g          448 t
ORIGIN      
        1 agggcggtgc cgacgctcgt cagcaggaac agcacgaaga tcgccatgat catcgccgag
       61 ccgcgctcgg cgtgccaacc gcatgccggc ttcatcagag cgcctttcgc agcaccacgt
      121 cgctgctgac ggtgtagcgg atgaactcgc ccgacgtcgg gtcgggcgcg ggcgactgtg
      181 ccgtgatctg caccgtcacg cgggtcggcg ggttggcgtc ggggacgaac atcggctcga
      241 tcgtcccgtc gccgtcggcg tcgttcgaga tgttgctcga cagcacgatg aacggttgcg
      301 tcgtgtcgct cgactcgcgc cagaccacct ctcgggtgct gccgaggtgc cggatcagga
      361 tctgttcgcc ggcatcgtcc gtgtccggat cctcgttgag gttggcgaag acctcgatct
      421 cgctgacgtc gtcgctcgtg tcggggtcga gattgaccgt cggcacgccc atgttcgtcg
      481 gatcccagcc ggcgctgcgc agcgcctgca cgaccatcga caacgtgttg cgcgcgttgg
      541 cctgtacctc ggccgtcatg cgctgggcct tgttgatccg cgcgttctgg atcagcaggc
      601 tggccaggcc accgagggcc atcaccagca ccgccacgct gacgagcagc tcgacgagag
      661 agaagccgcg ttgatcgttg cgcatcgttc tcatttcagc gcccccgcag cgtggtcagc
      721 gtgatttcct tccgctgtcc gacgacctgg cgcaccgcga tcgcccgaac cgtgattgtc
      781 ttgaccgacg gcggggccgc gtcgtccgtg atgcgccagc gcacgatgaa gtcggggtcg
      841 gacaggtcgg aatagccgga ctggctgacc gtcaggctgc cgccggcggc gaggctgacg
      901 aagctcgacg cgcggagcag ctccatccgt tcgaccgcga tcgctccgac cgagccgaaa
      961 tcggcgccgg tggcgtttcc ctgcatcgcg tagacgaaca tcggtgctgc cgccagcacg
     1021 ccgagcgcga tcaggaacag cgcgacgagc acctcgacca gcgagaagcc gcgctcgacg
     1081 cggactcgct cagtagatgt cgtagacgta cccatcatcg gttcctccgc cgccttgggg
     1141 gtcgtcgatc gtgacgtcgt cgatcggcgg cagctcgccg atgggtaccg ttccctcctc
     1201 gccgccgccc gagccgcccg agccgccgga gccgcccgaa ccgccggagc tcgccacctc
     1261 ggtcacgtag atgccgttgg tccagccgct gccgatccac cggtcgaccg tgatgccgcc
     1321 ggcgcccaag acggtgaccc ggttgtattc ctcgccgccg tagagcgcga cctggcccgt
     1381 gccgctcgcg accgatccgt cggccgtgaa tacgacctcg tagctgtcgg tcgtgccgat
     1441 ctgcgccagg ctgacgggca ctccgacgtc ggggcgcgcg aactgaacct gatcggtcag
     1501 gaccgtctcg tacagcacga cgtcgtcgct cgagtcggcc ggcgttccgt ttccgtcgaa
     1561 gacctgaagc gtgcgggtcg aggtgtcgag cacgaagcga cactggtgat tgcggctgac
     1621 cgcctcgata cgcgcgagct gcatgtagga ttggacgtcg tagaccgccc cccgcgcctt
     1681 ttcgcgcgag aaccagggca gcgcgtaggt cgccgaggcg acgaccgcaa ccgtgctgac
     1741 cgacatgacc aaggtcagct ccacgagtgt catacccttc tgatttttca tgactatgtc
     1801 cttctgattt tttgcaaatg tattggaaaa aaaactcctt ttcaattgcg cctttcgtaa
     1861 gatcggctga cgcgcgacgg atcggtcacg actcttcccg ttttgttaca aatcctgaag
     1921 acgggcaggt cgcgcgagcg atcggcacgc acgcgaaaaa gtcgccctgg acgtcgcgga
     1981 gaccgaaccg gtcagtcgaa agacaccccg ggccggcggg cgaggaactc gagcacggat
     2041 tccgccacgc cgggcgcctc ctcgacgcac gaattgaccg agatcccgcg gtaggaattg
     2101 ccgcacagga acagcccggc gtgggccgcg aggcgctcgc cgatccgctc gagccgcgcg
     2161 gggtggccga gctcgtactg ggggatgccg cgagaccaac gaacgatgcg gctgaagtag
     2221 ggccggatca tcaccgccat cgtccgcttg aggtcctggt ggaccagctc gagcagctgc
     2281 cggtcgtcga gctcgaccgc cgccgggtcg tgcgccccgc cgatcatcgt cgtcatcagg
     2341 ctcgccccgt cgggcgcgcg tccgtcgaag atctgcgaga tccacagcgt gccgaggatg
     2401 cgtggcccct ggccccgggg aacgaggaag ccgaagcccg tcggctggtc gccgagcgcg
     2461 agcgtgcgga aaccggtgtg gacgaccgcg acggaggccg acgggatcgc gccgagcagc
     2521 cggtgcagct cgggatccat cgactcgacc accggcgcgg cggaccacgc cggtgtcgcc
     2581 aggacgacgg cgtcgcactc gatcggcgcg ccttccggca gcaggatgcg aaagccgcgc
     2641 gagcccatgt ccgaaaggcg cacgaccggg ctgccgagcc gcagccgttc gccgagcgcg
     2701 gcggccaggg cgtccgtcag ctcctgcagg ccgtcgtgga acgaagt
//
LOCUS       DASPGN010000129         3725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_958471, whole genome shotgun sequence.
ACCESSION   DASPGN010000129 DASPGN010000000
VERSION     DASPGN010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958471"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VD788_03555"
     CDS             <1..714
                     /locus_tag="VD788_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75369.1"
                     /translation="EREKILDYMEQLTGTRFHTNFNMIGGARYDLTAELAREARQLAD
                     GFDAVLPELRSMTQAGEIFRRRTIGVGVIPRDLALSVGVTGPVLRGSGVGHDLRRDMP
                     YDAYPELDFDIPVRENGDAYDRFEVRMDEIAQSARLVRQLVDGLPAGPICSRKPLKNP
                     KTTRLAEGETYAAVESPRGELGFYLVADGSANPYRLKINAPSFCNLQVVPHIAPGLLL
                     ADVIATLGSLDPVLGDVDR"
     gene            711..1685
                     /gene="nuoH"
                     /locus_tag="VD788_03560"
     CDS             711..1685
                     /gene="nuoH"
                     /locus_tag="VD788_03560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HXV75370.1"
                     /translation="MNAWLLSALVSSIVLFVVMTCVAYLTWLERKVAARFQNRIGPFF
                     VGYPHGWLQPLADVVKLIVKEDITPRAVDRVLFNLAPLLVIAPALIGFAFIPFLGGTA
                     VVVHPLSFLFFLAFASLSLLGVFAAGWSSNNKYALISALRMVALLVSYEIPLLLSLLV
                     PALLVGSFDLVRIVEAQRGLWFIAAPGVGQVAFVVFFIAMLAESNKAPFDLTEAESEL
                     IAAYNVEYTGIKFALFYAGEYAHTFALAGLAATIFFGGYLGPALLPGPVWLLAKMFAL
                     FGLVLWVRWSLLRMRIDHALAFNWKILFPLSLVNLIAAGWWVVGRGPS"
     gene            1682..2227
                     /locus_tag="VD788_03565"
     CDS             1682..2227
                     /locus_tag="VD788_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXV75371.1"
                     /translation="MIGAIGTSVVGSLQAMGTTLRNFFRRANTVQYPKVQRPLPRQWR
                     GGTFALTIDGQTDEENCIGCRLCEYICPSQIIRVTLRKGEKRVNGLGATYCDLFTLDY
                     QACMQCELCIQVCPTDAIVMTRYLADCPTTRDGLFLGKEKLLEHGRALLARPEHESYA
                     TSSRLREWTDPARGQRTDGER"
     gene            2224..2706
                     /locus_tag="VD788_03570"
     CDS             2224..2706
                     /locus_tag="VD788_03570"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXV75372.1"
                     /translation="MSDVVFWSLAASLVLCSLGVVGSRNLYRAAYWLAGALVTTALFY
                     LALTAPLLAAVQILLYTGGVLTLVVYALVITASSDDTTRWRRPVPAALVGLLVFAVLA
                     SVALELGDGRAPRGGLDDGAVIGENLFTRYLVPFELLSLLLLGAIFGALAIARREGRS
                     "
     gene            2703..3002
                     /gene="nuoK"
                     /locus_tag="VD788_03575"
     CDS             2703..3002
                     /gene="nuoK"
                     /locus_tag="VD788_03575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HXV75373.1"
                     /translation="MTLERMLLLSFLIFAIGLFGVLVRRHLIGLLLSLELMFNSVNLA
                     ILAIAWQRGWLEGTILVLFGIAITVAEVAVGLSLFLLAERLGGTVRADDLSRMRG"
     gene            3004..>3725
                     /locus_tag="VD788_03580"
     CDS             3004..>3725
                     /locus_tag="VD788_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXV75374.1"
                     /translation="MELATVIVAAPFVAFALLGLVAPLRRAGRPAAWVSLAGSATSLA
                     TSLVLLARYRSRMAPDSSEFVWAPLDSVPSIRFGLLIDGLSVPMLALVSLVALLVQLY
                     SLGYMSEEKPAAFGRYFAYHSLFAAAMLGLVASHNTLQTYFFWELVGLGSYLLIGFWY
                     EKPVASRAAMKAFWVTRLGDVGFALGVILLWSAGGSFVFSELFEQAADGRLSGTLLVL
                     GVGGLFLGAMGKSAQFPLHVWLP"
BASE COUNT          458 a         1268 c         1256 g          743 t
ORIGIN      
        1 gagcgtgaga agatcctcga ctacatggag caactgaccg gaacacgctt tcacaccaac
       61 ttcaacatga tcggcggggc gcgctacgac ctgacggccg agctggcccg cgaggcccga
      121 cagctggcgg acggcttcga cgcggtgctg cccgagctgc gcagcatgac gcaagccggc
      181 gagatcttcc ggcgccgcac catcggcgtc ggcgtcatcc cgcgcgacct ggcgttgtcg
      241 gtcggcgtca ccggtccggt gctgcgcggc tcgggcgtcg gccacgacct gcgacgcgac
      301 atgccgtacg acgcctaccc ggagctcgat ttcgacatac cggtgcggga gaacggcgac
      361 gcctacgatc ggttcgaggt ccggatggac gagatcgcgc aatcggcccg cctcgtccgc
      421 cagctggtcg acggactgcc cgccggtccg atctgctcgc gcaagccgct gaagaacccc
      481 aagaccaccc gtctcgccga gggcgagacc tacgccgccg tcgaatctcc gcgcggcgag
      541 ctcggcttct acctggtggc cgacggcagc gccaacccct accggctgaa gatcaacgcc
      601 ccgtcgttct gcaacctgca ggtcgtgccg cacatcgcgc ccggtctgct gttggccgac
      661 gtgatcgcca cactcggctc gctcgatccg gtgctgggag acgtcgatcg atgaacgcct
      721 ggctgctctc ggccctcgtt tcgtcgatcg tgctgttcgt cgtgatgacc tgcgtggcct
      781 acctgacgtg gctcgagcgc aaggtagccg cccgattcca gaatcgcatc gggccgttct
      841 tcgtcggcta cccgcacggc tggttgcagc cgctggccga cgtggtcaag ctgatcgtca
      901 aggaagacat cacgccgcga gccgtcgacc gcgtgctgtt caacctggcg ccgctgctgg
      961 tcatcgcgcc cgcgctgatc ggtttcgcct tcatcccgtt cctcggcggt acggccgtcg
     1021 tcgtccatcc gctgtcgttt ctgttcttcc tcgcgttcgc ctcgctgtcg ctgctcggcg
     1081 tgttcgccgc cggctggtcg tcgaacaaca agtacgcgtt gatctcggcg ctgcgcatgg
     1141 tggcgttgct cgtctcctac gagatcccgt tgctgctgtc gttgctcgtg ccggcgctgt
     1201 tggtcggctc gttcgacctg gtgcgcatcg tcgaggcgca gcgtgggctg tggttcatcg
     1261 ccgccccggg cgtggggcag gtggcgttcg tcgtcttctt catcgcgatg ctggccgaat
     1321 ccaacaaggc gccgttcgat ctgaccgagg ccgagtcgga gctgatcgcc gcctacaacg
     1381 tcgagtacac ggggatcaag ttcgccctgt tctacgccgg cgagtacgcc cataccttcg
     1441 cattggccgg actggccgcg acgatcttct tcggcggcta tctcggcccg gcgttgctgc
     1501 cgggtccggt gtggctcttg gccaagatgt tcgcgttgtt cgggttggtg ctgtgggtgc
     1561 gctggagcct gttgcgcatg cgcatcgacc acgcgctcgc cttcaactgg aagatcctgt
     1621 tcccgctgtc gttggtcaac ctgatcgccg ccggctggtg ggtcgtcggg cgagggccat
     1681 cgtgatcggc gcgatcggca cgagcgtcgt cggctcgctg caggcgatgg gcaccacgct
     1741 gcgcaacttc ttccgacggg ccaacaccgt ccagtacccc aaggtccagc ggccgcttcc
     1801 gcgccagtgg cgcggcggca ccttcgcgct gacgatcgac ggccagaccg acgaagagaa
     1861 ctgtatcggc tgccgtctgt gcgagtacat ctgcccgtcg cagatcatcc gcgtcacgct
     1921 gcgcaagggg gaaaaacgcg tgaacggcct cggcgcgacc tactgcgacc tgttcacgct
     1981 cgactaccag gcgtgcatgc agtgcgagct gtgcatccag gtctgcccga ccgacgcgat
     2041 cgtgatgacg cgctacctgg ccgattgccc gaccacccgc gacgggttgt tcctcggcaa
     2101 ggagaagctg ctcgagcacg gtcgcgcgtt gctcgcgcgc cccgagcacg agtcgtacgc
     2161 caccagttcg cggttgcgcg agtggaccga cccggcgcgc ggccagcgga cggatggtga
     2221 gcgttgagcg acgtcgtctt ctggtcgctg gccgcgtcgc tcgtcctctg ctcgctcggc
     2281 gtggtcggct cgcgcaacct gtaccgcgcg gcctactggc tcgccggtgc gttggtcacg
     2341 accgcgctgt tctacctggc gctgaccgcg ccgctgctcg ccgccgtgca gatcctgctc
     2401 tacaccggcg gcgtgttgac gctggtcgtc tacgcgctgg tgatcaccgc cagctcggac
     2461 gacacgaccc gctggcggcg cccggtgccc gcggcgctgg tcggtctgct ggtcttcgcg
     2521 gtgctggcct cggtcgcgct cgagctgggc gacgggcggg ccccgcgcgg cgggctcgac
     2581 gacggcgcgg tgatcggcga gaacctgttc acccgctacc tggttccgtt cgagctgttg
     2641 tcgctgctgc tgctcggcgc cattttcggc gcgctcgcca tcgcccggcg cgagggacgg
     2701 tcgtgacgct cgagcggatg ctgctgctgt cgttcctgat cttcgcgatc ggcctgttcg
     2761 gcgtgttggt gcgccgtcac ctgatcggtc tgctgctgtc gctcgagctg atgttcaact
     2821 cggtcaacct ggcgatcctg gcgatcgcct ggcagcgcgg ctggctcgaa ggaacgatac
     2881 tcgtgctgtt cggcatcgcg atcacggtgg ccgaggtggc ggtcggtttg tcgctgttcc
     2941 tgctcgccga gcggctgggc ggcacggtgc gcgccgacga cctgtcgcgg atgcgtggtt
     3001 gacgtggaac tggcaaccgt catcgtcgcg gccccgttcg tcgccttcgc cctgctcggg
     3061 ctcgtggctc cgctgcgccg cgccggtcgc ccggcggcgt gggtctcgct cgctggcagc
     3121 gcgacgagtc tggcgacctc gctcgtgctc ttggcgcgtt accgcagccg gatggcgccc
     3181 gacagctcgg agttcgtctg ggccccactc gacagcgtac cgtcgatccg cttcggtttg
     3241 ttgatcgacg ggctgtcggt gccgatgctg gcgctcgttt cgctggtggc gttgctggtt
     3301 cagctctact cgctgggcta catgagcgag gagaagccgg cggcattcgg ccgctacttc
     3361 gcctaccaca gtctgttcgc cgccgcgatg ctgggcctgg tcgcctcgca caacacgctg
     3421 cagacctatt tcttctggga gctggtcggt ctcggctcgt acctgttgat cggcttctgg
     3481 tacgagaagc cggtcgcctc gcgcgcggcg atgaaggcgt tctgggtcac ccgactcggc
     3541 gacgtcggct tcgctctcgg cgtgatcctg ctgtggtcgg ccggcggcag cttcgtcttc
     3601 tccgagttgt tcgagcaggc cgcggacgga cgcctgtcgg gcacgctgtt ggtgctcggc
     3661 gtcggcggtc tgttcctcgg cgcgatgggc aagagcgccc agttcccgct gcacgtctgg
     3721 ctgcc
//
LOCUS       DASPGN010000130         4819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_139064, whole genome shotgun sequence.
ACCESSION   DASPGN010000130 DASPGN010000000
VERSION     DASPGN010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4819
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139064"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VD788_03585"
     CDS             complement(<1..612)
                     /locus_tag="VD788_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75375.1"
                     /translation="MRPLRIAVSALISLVCLAAGAGDAEIRVADPDDRPVADAVVALH
                     PTTGEAPDPRAPLRAVIDQIDEEFVPYVSAVRAGTAVSFPNRDAIRHHVYSFSEAKKF
                     ELPLYKGVPAEPVVFDRAGTVVLGCNIHDWMLAYVYVVGTPWFAVTDSSGRVVFADLP
                     TGEYRVEVQHPRLRDRVVNAAIDVDASVARLTVALRLQPDLRRG"
     gene            complement(652..1515)
                     /locus_tag="VD788_03590"
     CDS             complement(652..1515)
                     /locus_tag="VD788_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75376.1"
                     /translation="MWSSRRLASVGLAVWLGGGAAALAGGFAGVDSGVETTDNVTRAV
                     FAADRETETFGFVEPSAGWRLQPSTAHSLFLGARLRLAVAARADGLTHYAPEAAVRWQ
                     GKTRLGADAPRWIARLRAEWLGFRSDVRRGWHYEASIGYAAPAGRRFWVSAEANALTR
                     DADSAAFSQDALGLELRAEWRASRRWRVYAVYGARDGDVTSTATPAAIDPRVGPILAS
                     STARVEDDAFPGKVAYRLDGLSQRLELGANGALTRNGAFDLRYRFESTDAGAGIDYHA
                     SLVRAAWLLRF"
     gene            1602..2237
                     /locus_tag="VD788_03595"
     CDS             1602..2237
                     /locus_tag="VD788_03595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV75377.1"
                     /translation="MSLDDTQKKLNAYFAQKLESFGATPRGVDYNGPEAQAVRFEQLV
                     KVIDGSRKFSVIDYGSGYGAMFDFLDARGWQFDYYGVDLVEEMTIAGRAAHAGFANAR
                     FTTDERQLPVADYLVASAIFNVKLDTPVEQWRQSTLDTLRTMNRLCTKGLSFNMLTAY
                     SDADRVAARPDLFFGDPGFYFDFCKREFSRNVALLHDYDLYDFTILVRKAT"
     gene            2262..>4819
                     /locus_tag="VD788_03600"
     CDS             2262..>4819
                     /locus_tag="VD788_03600"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HXV75378.1"
                     /translation="MDQIEVRRFVHRRRRWLVAGAVLFACYTVGGFFVVPRIAEQQVV
                     AAFDETLGLGASVGRLRTNPFSFTIRANDFVLRDPTGDELLGFDELFVNWQLSSLFRW
                     AWTFKQVRLAGPRVVVIVEPDGSLNLRALVPRGEPRDAAAETGSLPRLLVRLLQVDAA
                     IVNFEDRTRPEPFRTVVGPAGFTLERFSTLPDRAGEFAFEANAESGARLAWQGTIAVN
                     PFHSRGSIELAGAQMPITKRYLQDRLRFHVPQGSTDSEWTYEVSIDQGGLNASVRDGS
                     VVVRDLRVTAEDGASEVLRLPELRIAGIGLAWPERRLSVDEITLGGGGLSVWRAADGT
                     INLLGPASERAPAEAGTAPPPFDWELSLGRLAVDDFAIGVEDRAVDPPFRTGLSQLAF
                     DLREVDNRVGSSFPFTLALAVDTGGTATSTGRLGALPAPTVDAAVRVDELSLVPLQAY
                     LDALARLRIDSGSVSIDGQLDSGAEELLRYRGRLELVELSCHDVQRDERLVAVGRLGA
                     EDLLLEATAARLRVASVELERPYGRLTIYEDGSTNVGGVLATGEESTETAGAADTARP
                     VTIEVGELRIGDGSADFADLSLPLPFASRIESLDGAVSEMATGGTSARVELEGTVDEY
                     GLARVEGSLDLFAPDRRSDVAVIFRNVEMNPLSPYSSKFAGYTIEEGRLSLDLRYRIE
                     DRTLDSDNAILIERLTLGEKVDSPDAPNLPVKLAVALLKDSRGNIELDIPVGGTLDDP
                     EFAYGKLVWKAIKNVLTKVATAPFRALAGLIGSDKDLEFVEFAPATSEIAPPAREKLD
                     ELARALVERPELVLEVAGVYDPEIDADGMRKQKLAASVAQRVAAGGTDPGAEPTL"
BASE COUNT          728 a         1645 c         1724 g          722 t
ORIGIN      
        1 cccgcgccgc aggtcgggct gcaatcgcag agcgacggtc agccgcgcga ccgacgcgtc
       61 gacgtcgatc gccgcgttta cgacgcggtc gcgcagccga gggtgctgca cttcgacacg
      121 atactcgccg gtggggaggt cggcgaagac gacccgtccc gacgagtcgg tgacggcgaa
      181 ccacggcgtg ccgacgacgt agacgtaggc cagcatccaa tcgtggatgt tgcagcccag
      241 caccaccgtg cccgcgcggt cgaacacgac cggctcggcc ggcacgccct tgtacagcgg
      301 gagctcgaac ttcttggctt ccgagaacga gtagacgtgg tgccggatgg cgtcgcgatt
      361 ggggaaggag accgcggtgc cggcgcgaac ggcgctgacg tatggcacga actcctcgtc
      421 gatctgatcg atgacggcgc ggagcggcgc gcgcggatcg ggcgcctccc cggtcgtcgg
      481 gtggagcgcg acgaccgcat cggcgaccgg ccggtcgtcc gggtcggcga cgcggatctc
      541 ggcgtccccg gcaccggcgg cgaggcagac gagcgagatc agcgccgaga cggcaatccg
      601 cagcgggcgc atcgcggacg aggttactcc attccgcacg gggccggtcc gtcagaaccg
      661 caggagccag gctgcgcgca cgaggctcgc gtgatagtcg atcccggctc ccgcgtcggt
      721 ggattcgaag cgataccgca ggtcgaacgc cccgttgcgc gtcagcgcgc cgttggcgcc
      781 gagctcgagc cgctgcgaca gcccgtccag ccggtaggcg accttgccgg ggaaggcgtc
      841 gtcctcgacc cgcgcggtcg agctcgccag aatcggcccg acacgcggat cgatcgccgc
      901 gggggtcgcc gtcgaggtca cgtcgccgtc gcgcgcaccg tagacggcgt agacccgcca
      961 gcgtcgagac gcgcgccact cggcgcgcag ctcgagcccg agcgcgtcct ggctgaacgc
     1021 cgcggagtcg gcgtcgcgcg tgagcgcgtt cgcctccgcc gacacccaga agcggcggcc
     1081 ggcgggcgcg gcgtagccga tgctggcctc gtaatgccag ccccggcgca cgtcgctgcg
     1141 aaacccgagc cactcggcac ggaggcgcgc gatccaacgc ggcgcgtcgg cgccgagccg
     1201 cgtcttgccc tgccagcgca ccgccgcctc cggcgcgtag tgcgtcagcc cgtcggcccg
     1261 cgcggccacc gccagcctca gcctggcacc gagaaacagg ctgtgggcgg tcgagggttg
     1321 caggcgccaa ccggcggagg gctcgacgaa gccgaacgtc tcggtctcgc ggtccgcggc
     1381 gaagacggcc cgggtgacgt tgtccgtcgt ctcgaccccc gagtcgacac cggcgaaacc
     1441 gccggcgagg gcggcggctc cgccaccgag ccagaccgcg agaccgacgg acgcgagacg
     1501 ccgcgaggac cacatcgtct ccgaaaacta ccacgtctct ccacgcgaac ccgctggacg
     1561 atgcggtttg gcgtatcttg gggcgacggg ggcggtgtac ggtgagcctc gacgataccc
     1621 agaagaagct gaacgcgtac ttcgcgcaga agctggaatc gttcggcgcg acgccgcgcg
     1681 gcgtggacta caacgggccg gaagcacagg cggtccgctt cgagcaactg gtcaaggtga
     1741 tcgacggctc gcggaagttc agcgtcatcg actatggcag cggctacggc gcgatgttcg
     1801 atttcctcga cgcgcgcggc tggcagttcg actactacgg tgtcgacttg gtcgaggaga
     1861 tgacgatcgc cgggcgtgcg gcgcacgcgg gctttgccaa cgcgcggttc acgaccgacg
     1921 agcggcagct gccggtcgcc gactacctgg tggcgagcgc gatcttcaac gtcaagctcg
     1981 acacgccggt cgagcagtgg cggcagtcga cgctcgacac gctgcgcacg atgaatcgac
     2041 tgtgcaccaa gggtctctcg ttcaacatgt tgaccgcgta ctcggatgcg gaccgcgtcg
     2101 cggcgcgacc ggatctcttc ttcggagatc cggggttcta tttcgatttc tgcaagcgcg
     2161 agttctcccg caacgtcgcg ttgctccacg attacgacct ctacgatttc acgattctgg
     2221 tgcgcaaggc gacctgatcg atcgacgagg aggcgatcgg cgtggaccag atcgaggtcc
     2281 ggcgattcgt tcaccggcgc cgcaggtggc tcgtcgccgg tgcggtgctg ttcgcctgct
     2341 acacggtcgg cggtttcttc gtcgtgcccc ggatcgccga gcagcaggtc gtcgccgcct
     2401 tcgacgagac gctcgggctg ggcgccagcg tcggtcgcct gcggaccaac ccgttcagct
     2461 tcaccatccg ggccaacgac ttcgtgctgc gcgatccgac cggcgacgag ctgctcggct
     2521 tcgacgagct gttcgtcaac tggcagctct ccagcctgtt ccgctgggcc tggacgttca
     2581 agcaggtccg gctggccggc ccgcgcgtcg tcgtgatcgt cgaaccggac ggcagtctca
     2641 acctgcgcgc gctcgttccc cggggcgagc cgcgggacgc cgccgccgag accggttcgc
     2701 tgccgcggct gctcgtccgt ctgctgcagg tcgacgcggc gatcgtgaac ttcgaggatc
     2761 gcacgcggcc cgagccgttc cgcacggtcg tcggccccgc cggcttcacc ctggagcgct
     2821 tcagcacctt gcccgatcgc gcgggtgagt ttgcgttcga agccaacgcc gagtcgggcg
     2881 cgcggctggc gtggcaaggc acgatcgcgg tcaatccgtt ccacagccgg ggttcgatcg
     2941 agctggccgg ggcccagatg ccgatcacca agcgttacct gcaggaccgg ctgcgcttcc
     3001 acgtgcccca gggcagcacc gattccgaat ggacctacga ggtgtcgatc gaccagggcg
     3061 gtctgaacgc gtcggtccgc gacggctcgg tcgtggtgcg cgatctgcgg gtgacggcgg
     3121 aggacggcgc gagcgaggtc ttgcgactac ccgagctgcg catcgccggg atcggtctgg
     3181 catggcccga gcggcggctg tcggtcgacg agatcacgct cggcggcggt ggtctgtccg
     3241 tctggcgcgc cgccgacggg acgatcaatc tgctcgggcc ggcgagcgag cgggcccccg
     3301 cagaggctgg gaccgcgccg ccgccgttcg actgggagct gtcgctcggc cgtctggcgg
     3361 tcgacgactt cgcgatcggc gtcgaggatc gagcggtcga cccgccgttt cgcaccggcc
     3421 tctcgcagct cgcgttcgat ctgcgcgagg tcgacaaccg agtgggcagc agcttcccgt
     3481 tcacgctggc gctggccgtc gataccggcg gcacggcgac gtcgacggga cggctcggcg
     3541 cgctgcccgc accgaccgtc gacgccgcag tccgagtcga cgagctgtcg ctcgtcccgc
     3601 tgcaggccta cctcgacgcg ctggcgcggc tgcggatcga ttcggggagc gtctcgatcg
     3661 acggtcagct cgactccggc gccgaggagc tgctgcgcta ccgcggccga ttggagctgg
     3721 tcgagctgtc ctgccacgac gtgcaacgcg acgaacgtct ggtcgccgtc ggtcggttgg
     3781 gtgcggagga cctgctgctc gaggcgaccg cggcacgcct gcgcgtcgcg agcgtcgagc
     3841 tcgagcggcc ctacgggcgg ctgacgatct acgaggacgg ctcgaccaac gtcggcggcg
     3901 tgctcgcgac gggggaggag tcgacagaaa ccgccggtgc cgccgacacc gcccggcccg
     3961 tcacgatcga ggtcggcgag ctgcgcatcg gcgacgggtc ggccgacttc gccgatctgt
     4021 cgctgccgct gccattcgcc tcgcgcatcg aatcgctcga cggcgccgtg tcggagatgg
     4081 cgaccggcgg tacgtcagcg cgcgtcgagc tcgagggcac cgtcgacgag tacggtctgg
     4141 cgcgggtgga ggggagcctg gacctgttcg caccggaccg ccggtcggac gtggcggtga
     4201 tcttccgcaa cgtcgagatg aacccgctgt cgccctattc gtcgaagttc gcgggctaca
     4261 cgatcgaaga gggccgcctg tcgctcgatc tgcgttatcg catcgaggat cgcacgctcg
     4321 actcggacaa cgcgatcctg atcgagcggt tgacgctcgg cgagaaggtc gactcacccg
     4381 atgcgccgaa tctcccggtc aagctcgccg tcgcgttgct caaggattcg cgaggcaaca
     4441 tcgagctcga cattccggtc gggggaacgc tcgacgatcc cgagttcgcc tacggcaagc
     4501 tggtgtggaa ggcgatcaag aacgtgttga ccaaggtggc gaccgcaccg ttccgtgcgc
     4561 tggccgggct gatcggctcg gacaaggatc tcgagttcgt cgagttcgca ccggccacga
     4621 gcgagatcgc gcccccggcc cgggagaagc tcgacgagct cgcccgcgcg ctagtcgaac
     4681 gcccggagtt ggtactcgag gtggcgggag tctacgaccc ggagatcgac gccgacggca
     4741 tgcggaagca gaagctcgcg gcctcggtcg cgcaacgcgt cgcggccggc ggaaccgacc
     4801 cgggcgccga gccgacgct
//
LOCUS       DASPGN010000131         5202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1310083, whole genome shotgun sequence.
ACCESSION   DASPGN010000131 DASPGN010000000
VERSION     DASPGN010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310083"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /gene="asnB"
                     /locus_tag="VD788_03605"
     CDS             <1..1766
                     /gene="asnB"
                     /locus_tag="VD788_03605"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXV75379.1"
                     /translation="PQLALGFRRLAVIDLDTGNQPIALDGDRAVIVLNGEIYNFRELK
                     SGPLAGEPFRTKGDVEVALRLYATRGLDAIRLLDGMFALAVWDASRETLLLARDRFGI
                     KPLFFVRDGPSVAFGSELSTLLAAGYPQRPRLDPGQLRHYLAQRYPDPRRAPLDGVEL
                     VPPGTVIELSQDGERRTRYWDPPSREISDGPDDEALARLDSLLVAAVRRQLVADVPLG
                     VFLSGGVDSGTIAAIAASAGGAPLKTFSVGFDDSGGEVADERPRAALAARHLSTEHHE
                     LSIDPRQVARDLPAILAALDGPLADPTVVPSWYLSALARREVVVALSGEGADELFGGY
                     DRVRFDLWIDRIGPVGRRWVPRAMAMAGRMPSDRLRARLTMPAGLERQLDWSRTFTTS
                     EIDALCVEPTPAEDEETLQVWRERARRWAIRALDDPINARLAVDRELFLPGDLLPKVD
                     RMSMAHSLEVRVPFLDNELADFVLAQPGSRKIRGGTTKWMLRRIARERLPPGAWRAPK
                     TGFDVPVSRWLRGPLREPLCELLSARAVRRRGLFRAETVSTMIDQHLDEVADHGMRLW
                     TLLALEGWMLSALDRPSGSGR"
     gene            1763..2965
                     /locus_tag="VD788_03610"
     CDS             1763..2965
                     /locus_tag="VD788_03610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV75380.1"
                     /translation="MTTRSMAVTMLSHVASPAAPTGAERSLALLASGLAERGHRVRVI
                     TPGPWSLGDALTRTGVAVETVATRPCWLTYYERRPWPLAAANWLRWAVASGAGRRLEQ
                     RLRELDPDVVHVNCLPHLSGAAAARAAGIPVVWHLREILPPGARRRWFARRLEHDASR
                     VVAVSQAVADWVRAERVALPLDVVHNGVEPPRSPPPAADARRRFGLGAADVVVGLFGQ
                     LLPHKAALDFVRAATAVAGRTPGASFLIAGDGSRAYLARIDRAIAAGGHRGRIVRLAG
                     RADPTELFAAADIVALTTRTPDPFPRSVLEAMAWGRPVVAYRSGGVPEMVEHDRTGLL
                     VAPGDVAGLAAALSALIDDEARRTAMGRRAIERVRERFTLVRHVERIERLLELARRPA
                     ASSANGVL"
     gene            2979..4241
                     /locus_tag="VD788_03615"
     CDS             2979..4241
                     /locus_tag="VD788_03615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV75381.1"
                     /translation="MRRLLLIAYYFPPIAGSGTFRPLRISRYLPAHGWAVSVLSVNTT
                     VRLVKDPALADEVRPDTTVVRTATLEPRNLELALRRIGMGRLAGRLESWLLLPDEQRG
                     WVPFAVAAGRRLLRGEPHHALLSTSSPYSTHLVGRALAASGLPWIADFRDEWTTNPYL
                     IERYPTAWHAGHNRRLECRVLRAADAVVSVSAPWAANHRSLVPDEPAEKFHVLPNGYD
                     PQQFAGRPWSRPDRFRVLYTGTFYGIRRPEALIDGVGTAIERGLVPADRLELVLMGQG
                     NTGIDPRALPPGVLTTIEHRPQREAIDEMYRAAVLVLVVPRAGGEGNHTGKLFNYLAV
                     GAPILALAPEPNVAADLVRDCAGGIVVPPDDPAAVADGLGHLFRMWSGEEPTPPRRVE
                     RIAEYAADRQAGRLAALLDRLVDRRARG"
     gene            4306..>5202
                     /locus_tag="VD788_03620"
     CDS             4306..>5202
                     /locus_tag="VD788_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HXV75382.1"
                     /translation="MLIRQGRVVVDDMPVPIPGPGEVLVRTAHSVISAGTELASIEGS
                     ATREPVSRWLNRLRKAGEVAALALDRGVTETRSAVAARLEGPSVACGYSLAGRVERVG
                     RDVTDLVAGQRVACAGASSAVHAEYVAVPRNLVAVVPDGLDSPSAAFATLGAIAMHGV
                     RQADVRLGEFVAVVGCGLIGQLTVALLHAAGCRVLASDLDAARVERASALGIERGIVA
                     GREEFARAVNQATGGRGADAVILTAATPSSEPIRQAVDALRRRGRVVVVGAVGMEIER
                     APFYHKEVELRIACSYGPGRHDP"
BASE COUNT          626 a         1891 c         1900 g          785 t
ORIGIN      
        1 cgccgcagct cgcgctcggc ttccgccggc tggcggtgat cgacctcgac accggcaacc
       61 agccgatcgc gctcgacggt gaccgggccg tgatcgtgct caacggcgag atctacaact
      121 tccgcgagct gaagagcggt ccgctcgccg gcgagccgtt ccgcaccaaa ggcgacgtcg
      181 aggtcgcgct gcggctgtac gcgacgcgcg gtctcgacgc gatccggctg ctcgacggca
      241 tgttcgccct cgccgtctgg gacgcctcgc gcgagacgct gctgctggct cgcgaccgct
      301 tcggcatcaa gccgctgttc ttcgtgcgcg acggaccgtc cgtcgccttc ggatcggagc
      361 tgtcgaccct gctcgccgcc ggctatccgc agcggccgcg tctcgacccg gggcaactgc
      421 gtcactacct ggcccagcgt tatcccgatc cgcgccgggc gccgctcgac ggggtcgagc
      481 tggtgccgcc ggggacggtc atcgagctgt cccaagacgg cgagcgccgg acgcgatact
      541 gggatccccc gagccgggag atctcggacg gcccggacga cgaggcactg gcccggctcg
      601 attcgctgct cgtcgccgcc gtccgccggc agctcgtggc cgatgtcccg ctcggtgtct
      661 tcctcagcgg cggcgtcgac tcggggacga tcgccgcgat cgccgcgagc gcgggcggcg
      721 ctccgctcaa gacgttctcc gtcggcttcg acgactccgg cggcgaggtg gccgacgagc
      781 gtccgcgcgc cgcgctcgcc gctcgtcatc tgtcgaccga gcaccacgag ctttcgatcg
      841 acccgcgtca ggtcgcgcgc gatctgcccg cgatcctcgc cgcgctcgac ggtccgctgg
      901 ccgacccgac cgtcgtaccg agttggtacc tgtcggcact cgcacgccgg gaagtcgtcg
      961 tcgcgctgtc gggcgagggc gccgacgagc tgttcggcgg ctacgatcgg gtccgtttcg
     1021 atttgtggat cgaccggatc ggaccggtcg gccggcgctg ggtgccgcgc gcgatggcga
     1081 tggcgggccg catgccgtcc gatcgcctgc gcgcccggct gacgatgccc gccggtctcg
     1141 agcgccagct cgactggtcg cgcaccttca ccaccagtga gatcgacgcg ttgtgtgtcg
     1201 agccgacacc cgcggaggac gaggaaacgc tgcaggtatg gcgggaacgc gcccgacgct
     1261 gggcgatacg cgcgctcgat gacccgatca acgcgcggtt ggccgtcgac cgcgagctgt
     1321 tcctgcccgg cgacctgctg cccaaggtcg atcggatgag catggcgcac tcgctcgagg
     1381 tgcgcgtgcc gttcctcgac aacgagctgg ccgacttcgt cctggcgcaa ccgggctcgc
     1441 gcaagatccg cggcggtacg acgaagtgga tgttgagacg gatcgcgcgc gagcggctgc
     1501 cgccgggggc ctggcgcgcg ccgaagaccg gtttcgacgt gccggtgtcg cgctggttgc
     1561 gcgggccgct gcgcgagccg ctgtgcgagc tgttgtcggc gcgagccgtg cgacgacgag
     1621 gtctgttccg cgccgagacg gtctcgacga tgatcgacca gcacctggac gaggtcgccg
     1681 atcacgggat gcggctgtgg accctgctgg cgctcgaggg atggatgctg tcggcgctcg
     1741 accggccgtc ggggagcggt cggtgacgac gcgatcgatg gcggtgacga tgctgtccca
     1801 tgtcgcctcg cctgccgccc cgaccggcgc cgagcgtagt ctcgcgctgc tcgcgagcgg
     1861 gctcgccgag cgcggccacc gcgtgcgggt gatcacgccc gggccgtggt cgctcggtga
     1921 cgcgttgacc cgtacgggtg tcgcggtcga gacggtcgcc acgcggccgt gctggctgac
     1981 ctactacgag cgccgaccgt ggccgctcgc ggcggccaac tggctgcggt gggcggtggc
     2041 gagcggcgcg ggccgccgtc tcgagcagcg actgcgcgag ctcgatcccg acgtggtcca
     2101 cgtcaactgt ctgccgcatc tgagcggtgc ggccgcggcg cgcgcggccg ggattcccgt
     2161 cgtctggcac ctgcgcgaga tcctgccccc gggcgcccgg cgccggtggt tcgcgcgccg
     2221 gctcgagcac gacgcgagcc gcgtcgtcgc cgtcagccag gcggtcgccg actgggtccg
     2281 agcggaacgc gtggcactgc cgctcgacgt ggtgcacaac ggggtcgagc cgccgcgctc
     2341 gccgccacca gcggcagacg cgcgacgccg attcggcctc ggcgccgccg acgtcgtcgt
     2401 cggcttgttc ggccagctgc tgccgcacaa ggcggcgctc gacttcgtcc gcgcggcgac
     2461 ggcggtcgcc ggccgcacgc cgggcgcgag cttcctcatc gccggcgacg gcagccgggc
     2521 ctatcttgca cggatcgacc gtgcgatcgc cgccggcgga caccgcggcc gcatcgtccg
     2581 gctggcgggt cgggccgacc cgaccgagct gttcgccgcc gccgacatcg tcgcgctcac
     2641 gacgcgcaca cccgatccgt tcccgcgcag cgtgctcgag gcgatggcgt ggggccgccc
     2701 ggtcgtggcc tatcgttcgg gcggtgtgcc cgagatggtc gagcacgacc gaaccggtct
     2761 cttggtcgcg ccgggcgacg tcgccggctt ggccgccgcg ctgtccgcgc tgatcgacga
     2821 cgaggctcgc cggacggcga tggggcgccg cgcgatcgag cgcgtccgcg agcgattcac
     2881 gctcgtgcgg cacgtcgagc ggatcgagcg gttgctcgag ctcgcgcgcc ggccggccgc
     2941 gtcgagcgcg aacggcgtgt tatgatcgcc gcgccccgat gcgacggctc cttctgatcg
     3001 cctactactt cccgccgatc gccggttcgg ggacctttcg tccgctgcgc atcagccgct
     3061 accttccggc gcacggctgg gcggtctccg tgctcagcgt caacacgacc gtgcgcctcg
     3121 tgaaagaccc tgcgctggcc gacgaggtcc gaccggacac gacggtcgtg cgcacggcga
     3181 cgctcgagcc gcgcaacctc gagctcgcgt tgcgcaggat cggcatgggc cggctcgccg
     3241 ggcggctcga gtcgtggttg ctgcttccgg acgagcagcg cggatgggtc ccgttcgccg
     3301 tcgcggccgg ccggcgcctg ctgcgcgggg agccgcacca tgcgctgttg tccacctcgt
     3361 ccccgtactc gacgcacctc gtcgggcgcg cgctggcggc ctccggtctt ccgtggatcg
     3421 cggacttccg cgacgagtgg acgaccaacc cgtatctgat cgagcgctac ccgacggcct
     3481 ggcacgcagg gcacaaccgg cggctcgagt gtcgggtgct gcgcgcggcc gacgcggtgg
     3541 tcagcgtgtc ggccccgtgg gcggccaacc atcggtccct cgtccccgac gagcccgcgg
     3601 agaagttcca cgtcctgccg aacggctacg acccgcagca gttcgccggc cgtccgtggt
     3661 cgcggcccga ccgcttccgc gtgctctaca ccggcacgtt ctacggcatc cggcgaccgg
     3721 aggcgttgat cgacggcgtc ggtacggcga tcgagcgcgg gctcgtcccc gccgatcggc
     3781 tcgagctcgt gctgatgggt cagggcaaca ccgggatcga cccgcgtgca ctgccgcccg
     3841 gcgtgttgac gacgatcgag caccgcccgc aacgcgaggc gatcgacgag atgtaccgag
     3901 cggccgtgct ggtgctcgtc gttccgcgcg ccggcggcga ggggaatcac accggcaagc
     3961 tgttcaacta cctggccgtc ggagcgccga tcttggcctt ggcccccgag ccgaacgtcg
     4021 cggccgacct cgtgcgcgac tgcgccggtg gtatcgtggt gccgcccgac gacccggcgg
     4081 ccgtcgccga cgggctggga cacctgttcc gcatgtggag cggtgaggag ccgacgccgc
     4141 cgcggcgcgt cgaacggatc gccgagtacg cggccgaccg gcaggccggc cgtctggcgg
     4201 cgttgctcga tcgtctcgtc gatcggcggg cccgcgggtg aggcggagct tgcccgtcgc
     4261 cggggccgca tatatctatc ttcacggcgc gtaccgatga aacaggtgct gatcagacag
     4321 ggtcgagtcg tcgtcgacga catgccggtg ccgatccccg gccccggcga agtgttggtg
     4381 cgcaccgccc actccgtcat cagcgccggc accgagctcg cgtcgatcga gggatccgcg
     4441 acgcgcgagc cggtctcgcg ctggctgaat cgcctgcgca aggccggcga ggtcgcggcg
     4501 ctggcgctcg atcgcggcgt gaccgagacg cgcagcgcgg tcgcggcgcg gctcgagggt
     4561 ccgtcggtcg cctgcggcta cagcctcgcc ggtcgcgtcg agcgcgtcgg ccgcgacgtc
     4621 accgacctcg tcgccgggca acgggtcgcc tgcgccggtg cctcgtcagc ggttcacgcc
     4681 gagtacgtcg ccgtcccgcg caacctcgtc gcggtcgtgc cggacggact ggacagcccg
     4741 agcgccgcgt tcgccacgct cggcgcgatc gcgatgcacg gtgtgcgcca ggccgacgtc
     4801 cgtctcggcg aattcgtcgc cgtcgtcggt tgcgggctca tcggtcagtt gacggtcgcg
     4861 ttgctccacg cggcgggctg ccgcgtgctg gccagcgatc tggatgcggc ccgcgtcgag
     4921 cgcgcttcgg cgctcggcat cgaacgcggc atcgtcgccg gtcgagagga gttcgcgcgc
     4981 gccgtgaacc aggcgaccgg cggtcgcggg gccgacgcgg tgatcctcac cgcggcgacc
     5041 ccctcctccg agccgatccg ccaggcggtc gacgcactgc gccgccgcgg gcgggtcgtc
     5101 gtcgtcggcg cggtcggaat ggagatcgag cgcgctcctt tctatcacaa ggaggtcgag
     5161 ctgcgtatcg cctgctccta cggacccggc cgccacgatc cg
//
LOCUS       DASPGN010000132         5826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_22513, whole genome shotgun sequence.
ACCESSION   DASPGN010000132 DASPGN010000000
VERSION     DASPGN010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22513"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1339)
                     /gene="metK"
                     /locus_tag="VD788_03625"
     CDS             complement(188..1339)
                     /gene="metK"
                     /locus_tag="VD788_03625"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXV75383.1"
                     /translation="MQISGRHLFTSESVTEGHPDKVSDQISDAILDAILEKDPTGRVA
                     CETLVTTGLCLVAGEITTHAYIDIPSVVRRTIQEVGYDDPHYGFDYETCAVLTSIDEQ
                     SGDIAMGVDTGGAGDQGLMFGYACRETEQLMPLPITLAHQLARQLADCRRAGAPSFLR
                     PDGKSQVTVEYENGAARRVDAIVVSTQHDPGVGRAEIEEAVREMIIKPVVPARYLDHE
                     TKIFVNPTGQFIIGGPKGDAGLTGRKIIVDTYGGMGHHGGGAFSGKDPTKVDRSASYM
                     ARCIAKNLVAAGLADRAEVQLAYAIGVADPVSVMVDTFGTGRLPEERLAQLVREHFPL
                     TPAGIIDHLELRRPVFRRTAAYGHFGRSEPGFTWEATQVAERLADEVKA"
     gene            complement(1436..4972)
                     /gene="dnaE"
                     /locus_tag="VD788_03630"
     CDS             complement(1436..4972)
                     /gene="dnaE"
                     /locus_tag="VD788_03630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXV75384.1"
                     /translation="MSDVTPGFVHLHNHTTYSLLDGAQRIDEMCARAAEDGQPAIAIT
                     DHGNLFGVMEFGKRAAKHGIKPIVGIEAYVAPESHQARQPSRVAGVGNKNYYHLILLA
                     ENYAGYKNLIRLASIGYLEGFYYRPRIDKQLLAQHSEGLVCLSACLAGEIPTFLNHDQ
                     YDRAVRAAGELRELFGPERFWLELQDHGIQAQHKVNVALPKLARELGLPMVASNDCHY
                     LLPDDHEAHDVLVCISTGSAVGGRASSVYTPQHYLKTRAEMAELFDWAPQAVENTLAI
                     AERCQFAFGKPTLQLPDFPVPAGYDLDGYFERMARDGLEWRMVELRRLQQAGRLARPL
                     EDYRKRLDEELAVIRQMGYSGYFLIVWDFIRFAREAGIPVGPGRGSAAGSLVAYSLRI
                     TDIDPLAYNLLFERFLNPERVSMPDIDIDFCFRKREKVIDYVTGKYGRRNVAQIITFG
                     TMAARAVIRDVGRVLEIPYAEVDRIAKLVPAAPGKEVTIDGAMADVPQLKEAYDKEPR
                     IKELLDVARRLEGLTRHASTHAAGVVIAPREIVEFAPLYRGTKDGDEITTQWAKDEIE
                     ECGLLKMDFLGLKTLTLIDDALASIERVSGARLDLDGVPLDDEAVYRLFGRARTSGIF
                     QFESEGMKDILRRLKPEKFEDLIALNALYRPGPIGGGLIDEFIKRRHGKVKVEYPHPM
                     LAPILEETYGVIVYQEQVMQIASLMGGYSLGEADILRRAMGKKKAAVMKAEKEKFLER
                     AMAKGIDRADGAKVFELMEYFAGYGFNKSHSAAYALVAFHTAWLKTHYPVHFMAALLT
                     NDKGNTDKLVKYINECREMGIEVLPPDVNASDIDFTVEGERIRFGLSAIKNVGEGAIR
                     AVIEARQREGRFESLHDLCGAVDARQVNKRVLEALVQSGAMDSFGARRSQLFHAIDVA
                     LEHGQKQRADREAGQVSLFGAAGETDRPAPLPLDDAPEWDEKTRLSGEKATLGFYVSG
                     HPLESYRDLLEEFRVCRSGALREMDSGTEVAVGGVIADLNRRKSRKGEWWASLQLEDL
                     EGQVEVLVFPKCYKTHEHDLDPERPALIAGRLEIDEDRLRVIADSVCPLDELRERNVN
                     AVHLSLDAGQLDEELVERIMHAVESHRGKAQLYFEVARPGAYRLVALAESSLRVAPSG
                     AFAREIESIVGPNKVRYRTTPLLRSGDGSSVP"
     gene            complement(4975..>5826)
                     /gene="guaA"
                     /locus_tag="VD788_03635"
     CDS             complement(4975..>5826)
                     /gene="guaA"
                     /locus_tag="VD788_03635"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HXV75385.1"
                     /translation="VMAMLLHRAIGENLSCIFVDNGLLRKNEAEQVSAAFRQRYHLQV
                     RQADAADRFLARLAGVEDPETKRKIIGNTFIEVFEEQAGLFGDARFLAQGTIYPDRIE
                     SASVRGPSATIKTHHNVGGLPERMRLELVEPLRDLFKDEVRRVGIELGLDAAFVNRHP
                     FPGPGLAVRIVGEVSKARVSLLQQADAIFIDELRRADLYDRTAQAFVVLLPIKTVGVM
                     GDGRTYEHVVALRAVETTDFMTANWARLPHEFLARVSSRIVNEVSGINRVVYDVTSKP
                     PSTIEWE"
BASE COUNT          952 a         2014 c         1847 g         1013 t
ORIGIN      
        1 gcgagcacgc ggcccgactc gacgagccgc gctcccccgg cgagcagcgg accgtcgagc
       61 acgccgaact cgtccggtcc gatccgctcg acaccggcga gcagccagac cgcggccaac
      121 ccgcagacga cgggtgccgc acggagcaag atgcggcgcg tcgctccccg atccgaacgt
      181 agtcgcgtca ggccttgacc tcgtcggcca gtcgctcggc gacctgcgtc gcctcccagg
      241 tgaaacccgg ctccgagcga ccgaaatgtc cgtaggccgc cgttcgccga aagaccggcc
      301 gccgcagctc gaggtgatcg atgatgccgg cgggggtcag cgggaagtgc tcgcgcacca
      361 gctgcgccaa ccgctcctcg ggcagacgac cggtgccgaa ggtgtcgacc atcaccgaga
      421 ccggatcggc cacgccgatc gcataggcca gctgcacctc ggcgcggtcg gcgagacccg
      481 cggcgacgag gttcttcgcg atgcagcgcg ccatgtacga cgccgagcga tcgaccttcg
      541 tcgggtcctt gcccgaaaac gcgcctcccc cgtggtgccc catcccgccg taggtgtcga
      601 cgatgatctt gcgcccggtc aagccggcgt cacccttcgg cccgccgatg atgaactggc
      661 cggtcggatt gacgaagatc ttcgtctcgt ggtcgaggta ccgcgcggga acgaccggtt
      721 tgatgatcat ctcgcgcacc gcctcttcga tctccgcacg gccgacgccg ggatcgtgct
      781 gggtcgatac gacgatcgcg tcgacacggc gtgccgcgcc gttctcgtac tcgacggtaa
      841 cctgcgactt gccgtcgggg cgcaggaacg acggcgcacc ggcgcgccgg cagtcggcca
      901 attgccgcgc cagctgatgg gccagcgtaa tcggcaacgg catcagctgc tccgtctcgc
      961 gacaggcgta gccgaacatc agcccctggt ctccggcgcc gccggtatcg acgcccatcg
     1021 cgatgtcgcc cgactgctcg tcgatcgagg tcagcacggc gcaggtctcg tagtcgaagc
     1081 cgtagtgcgg atcgtcgtaa ccgacctcct ggatcgtccg ccgcaccacc gacggaatgt
     1141 cgatgtaggc gtgggtcgtg atctcgccgg cgaccagaca cagcccggtc gtcaccagcg
     1201 tttcacaggc cacgcggccg gtcgggtcct tctcgagaat cgcatccagg atcgcgtccg
     1261 agatctgatc ggaaaccttg tccgggtggc cctcggtcac cgattcggag gtaaagagat
     1321 gtcggccgct gatctgcatg tcggatctcc tgagttggct gggatcgagc aagaaccgtg
     1381 aaaacagcag ttcatcctac caacggaccg ggcaggcatc aaacacgacc cggcgttatg
     1441 gcacacttga cccgtccccg ctccgcagca gcggcgtcgt ccggtagcgg accttgttcg
     1501 gtccgacgat cgactcgatt tcccgggcga acgcgccgga cggcgccacc cgcaagctcg
     1561 actcggcaag cgcgacgagg cgataggcgc cgggccgggc cacctcgaaa tagagttgcg
     1621 ccttgccgcg atgcgattcg accgcgtgca tgatgcgctc gacgagctcc tcgtcgagct
     1681 gccccgcgtc caagctgaga tgcaccgcgt tgacgttccg ttcgcgcagc tcgtccagcg
     1741 ggcagaccga gtcggcgatc acccgcaggc ggtcttcgtc gatctccagg cggccggcga
     1801 tcaacgccgg tctttccggg tcgaggtcgt gctcgtgcgt cttgtagcat ttggggaaga
     1861 ccagcacctc gacctgtccc tcgaggtcct cgagctgcaa cgacgcccac cattcaccct
     1921 tgcgcgactt ccgccggttg agatcggcga tcacgccacc gacggcgacc tccgttcccg
     1981 agtccatctc gcgcagcgcc ccgctgcgac agacgcgaaa ctcctcgagc agatcgcggt
     2041 agctctcgag cggatggccg gagacgtaga aaccgagcgt cgccttctcg ccgctcagtc
     2101 gagtcttctc gtcccattcc ggcgcgtcgt cgagcggcaa cggtgccggc cggtccgtct
     2161 cccccgctgc gccgaacaga ctcacctgcc ccgcttctcg atccgcacgc tgtttctggc
     2221 cgtgctcgag cgcgacgtcg atcgcatgga acagctggct gcgtcgcgca ccgaacgagt
     2281 ccatcgcgcc cgattggacc aacgcttcga gcacgcgctt gttcacctgg cgtgcgtcga
     2341 ccgcgccgca cagatcgtgc agcgactcga accgcccctc ccgctgtcgg gcctcgatca
     2401 ccgcgcggat cgccccctcg ccgacgttct tgatcgccga cagtccgaag cggatccgct
     2461 cgccctcgac ggtgaagtcg atgtccgagg cgttgacgtc cggcggtaat acctcgatcc
     2521 ccatctcgcg acactcgttg atgtacttga ccagcttgtc cgtattcccc ttgtcgttgg
     2581 tcaacaacgc cgccatgaag tggaccgggt agtgcgtctt gagccacgcg gtgtggaagg
     2641 cgacgagcgc ataggccgcc gaatgcgact tgttgaatcc gtagccggcg aagtactcca
     2701 tcaactcgaa gaccttggcg ccgtccgcgc ggtcgatgcc cttggccatc gcgcgctcga
     2761 ggaacttctc cttctcggcc ttcatcaccg cggccttctt ctttcccatc gcgcggcgca
     2821 ggatgtcggc ctcgcccagc gagtagccgc ccatcagcga cgcgatctgc atcacctgct
     2881 cctggtagac gatcacgccg taggtttctt cgaggatcgg tgccagcatc gggtgcgggt
     2941 attcgacctt gaccttgccg tggcggcgct tgatgaactc gtcgatcagc ccgccgccga
     3001 tcgggcctgg ccggtacagc gcgttgagcg cgatcaggtc ctcgaacttc tccggtttga
     3061 gccggcgcag gatgtccttc atcccttccg actcgaactg gaagatcccg gaggtgcgcg
     3121 cccgaccgaa cagacggtag accgcctcgt cgtcgagcgg cacgccgtca agatccagcc
     3181 gggccccgga gacgcgctcg atcgaggcca gcgcgtcgtc gatcagggtc aacgtcttga
     3241 gaccgaggaa gtccatcttc agcagcccgc attcctcgat ctcgtccttg gcccattgtg
     3301 tggtgatctc gtcgccatcc ttggtgccgc gatagagcgg agcgaactcg acgatctcgc
     3361 gtggagcgat caccacgccg gccgcatgcg tcgacgcgtg acgggtcaat ccctcgagcc
     3421 gccgtgcgac gtcgagcagc tccttgatgc gcggctcctt gtcgtacgcc tccttgagct
     3481 ggggcacgtc ggccatcgcg ccgtcgatcg tcacctcctt tcccggtgct gccggcacga
     3541 gcttggcaat gcgatcgacc tcggcatacg ggatctcgag cacgcggccg acgtcgcgga
     3601 tcaccgcgcg ggccgccatc gtgccgaagg tgatgatctg cgccacgttg cgtcggccgt
     3661 acttgccggt gacgtagtcg atgaccttct cccgcttgcg aaagcagaag tcgatgtcga
     3721 tgtcgggcat gctgacccgt tccgggttga ggaaccgctc gaacagcagg ttgtaggcca
     3781 acgggtcgat gtcggtgatg cgcaggctgt aggccaccag cgagccggca gccgaaccgc
     3841 gacccggacc gaccgggatc cccgcctcgc gcgcgaagcg gatgaaatcc cagacgatga
     3901 ggaagtagcc cgagtacccc atctgtcgga tgaccgcgag ctcctcgtcg agccgcttgc
     3961 ggtagtcctc gagcggccgc gccagacgtc cggcctgctg cagtcgtcgc agctcgacca
     4021 tccgccactc cagaccgtcg cgagccatcc tctcgaagta gccgtcgagg tcgtagccgg
     4081 ccggcaccgg aaagtccggg agctgcagcg tcggcttgcc gaacgcgaac tgacagcgct
     4141 cggcaatggc gagcgtgttc tcgacggcct gtggcgccca gtcgaacaac tccgccatct
     4201 cggcccgcgt cttcaggtag tgctgcggag tgtagaccga cgaagcacgc ccgccgacgg
     4261 cgctaccggt cgagatgcag accagcacgt cgtgcgcctc gtgatcgtcg ggcagcaggt
     4321 aatggcagtc gttgctcgcg accatcggca ggccgagctc gcgggcgagt ttgggcaacg
     4381 cgacattgac cttgtgctgc gcctggatcc cgtggtcctg cagctcgagc caaaagcgct
     4441 ccgggccgaa cagctcgcgt agctcgcccg cggcgcggac cgcccggtcg tactggtcgt
     4501 ggttgaggaa ggtcggaatc tccccggcca ggcaggccga caggcaaacc agcccttcgc
     4561 tgtgttgcgc cagcagctgc ttgtcgatgc gcgggcggta gtagaatccc tcgaggtagc
     4621 cgatgctggc cagccgaatc aggttcttgt aaccggcgta gttctcggcc agcagaatca
     4681 ggtggtagta gttcttgttg ccgacgccgg ccacgcggct cggctgacgg gcttgatgcg
     4741 actccggcgc gacgtaggcc tcgatgccga cgatcggctt gatgccgtgt ttggccgccc
     4801 gcttgccgaa ctccatcacg ccgaacagat tgccgtgatc ggtgatcgcg atcgccggct
     4861 ggccgtcttc cgccgcccgc gcgcacatct cgtcgatccg ttgggcgcca tccagcaacg
     4921 aataggtcgt gtgattgtgc aggtggacga atcccggcgt gacgtcggac atcttcactc
     4981 ccactcgatc gttgacgggg gcttgctggt cacgtcgtaa accacccggt tgatcccact
     5041 gacctcgttg acgatgcgcg aggagacgcg tgcgagaaac tcgtgcggga gccgggccca
     5101 gttggccgtc atgaaatcgg tggtctcgac cgcccgcagc gcgacgacgt gctcgtaggt
     5161 ccggccgtcg cccatcacgc ccaccgtctt gatcggcagc aggacgacga aggcctgcgc
     5221 cgtgcggtcg tacaggtcgg ctcgccgcag ctcgtcgatg aagatcgcgt cggcctgctg
     5281 cagcagcgaa acccgcgcct tgctcacctc gccgacgata cgcaccgcca gccccgggcc
     5341 gggaaacgga tgacggttga cgaacgcggc atcgagaccg agctcgatcc cgacgcgacg
     5401 cacttcgtcc ttgaacaggt cgcgcagcgg ctcgacgagc tcgagcctca tccgctccgg
     5461 cagaccgccg acgttgtgat gcgtcttgat cgtcgccgac ggaccgcgca ccgacgccga
     5521 ttcgatccga tccgggtaga tcgtgccctg cgccaggaac cgggcgtcac cgaacaggcc
     5581 cgcctgctcc tcgaacactt cgatgaacgt gttgccgatg atcttccgtt tcgtctccgg
     5641 gtcctcgacg ccggccagcc gagcgaggaa ccggtcggcg gcgtctgcct gtcgcacctg
     5701 caggtggtag cgctggcgga atgcggcgct gacctgctcg gcctcgttct tgcggagcag
     5761 gccgttgtcg acgaagatgc aactgaggtt ctcgccgatc gctcggtgca acagcatcgc
     5821 catcac
//
LOCUS       DASPGN010000133        18228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_373813, whole genome shotgun sequence.
ACCESSION   DASPGN010000133 DASPGN010000000
VERSION     DASPGN010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 18228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18228
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373813"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..847)
                     /locus_tag="VD788_03640"
     CDS             complement(221..847)
                     /locus_tag="VD788_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75386.1"
                     /translation="MTNGRRAAGLLLSAVWFAAPGAAAQNAVHHADGWQYSFGNLGYF
                     DGRTGDGLRGSDAGFAIGGGVGRRHARHLAFAFDSLFAGRSYDTPTELQFADDEMFLT
                     NLVLTGNVQAIVPFWLFEGYVGFGLGLMISELEVPSSVIWSETATSRSSFDLSTQILV
                     GIDFVYTRRSRIGVEFRNIFATADFDELSRGGVDVGGRLVAIKIKLPL"
     gene            941..1396
                     /locus_tag="VD788_03645"
     CDS             941..1396
                     /locus_tag="VD788_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75387.1"
                     /translation="MRAKAAEPLAAERVDPAERRLAVALRLGGSLLLLAFGAVLLPTQ
                     SMTATHRWLTMGEFPESPLADYLTRSISLLYGIKGGMYWVLATDVRRYAPVIRYVGWT
                     TIGFGVLMVAIDYRAGMPLFWTLSEGPPIAAIGMLFLLLLRRVDAVGRA"
     gene            1445..3028
                     /locus_tag="VD788_03650"
     CDS             1445..3028
                     /locus_tag="VD788_03650"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HXV75388.1"
                     /translation="MTDDADRPAADERPASRLIETLSAVLGNLARPSAVLKTILHQAV
                     AQTSAERGVFVEVGRSGRMTYRVLYRFEREELAGPAGSFSATIFARVLETGEGIVLGN
                     AMEDSRFDGSGSIHDLNLVSVMSLPIRVGGEIAALVHLEHTRPDHFTERHRRLVESLL
                     ELSGPVLAALRAGADVIQQNDALRQTASRAREHLESDRDQLTRDWSFGRFVGRSAAVR
                     ALGHSVAKAARSESPVLLTGETGTGKSILARVLHHQSRRRNAAMVTVFCPSLERGMVE
                     AELFGHRRGAFTGAVADRVGKVQAAEDGTLFLDEIGELPLEIQPKLLRLLQEKTYERV
                     GDAVERIANVRVIAATNRDIRREVQIGRFRRDLYERLNFVPIRVPPLRDRRYDIPVLL
                     RHCLDQHDQGRWIEVSPDAERYLMELDFAWPGNVRHVEQMAARLALERFVEPVTVGDL
                     ESVIDADREAADDGEGSRPSERELEAGLPAFLAHAEKRWLVEALRRYSDLSKKELARK
                     LNIGESTLHKKIRQYGISA"
     gene            complement(3064..4152)
                     /locus_tag="VD788_03655"
     CDS             complement(3064..4152)
                     /locus_tag="VD788_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV75389.1"
                     /translation="MKKVVTILIGLVALSLFAGTIFYLYSKSQEQPVTYRTESPSVTD
                     IVYKTVATGSVVPRKEVEIKPQVSGVVDELFVEAGDRVEAGARIARIRIIPDMVNLAN
                     AETRVNRARINLENAEIDIERSRRLASEGVVARTELQQFELAYDSAKEELAASVDNLE
                     LIKKGTRAKAGESTNTLVEATIEGMVLEVPVEVGDSVIEANTFNEGTTIATIADMDEL
                     IFEGLVDESEVGKIRPGMELLLTIGAIEGKTFKATLEHIAPKGVEENGAIQFEIRAAL
                     AQQDGAFVRANYSANADIVLDRRDEVLAISEGLLQFDGDVTYVEVETADQQFERREIE
                     TGLSDGIQIEVVGGLTADDRVKDPNSAG"
     gene            complement(4186..5442)
                     /locus_tag="VD788_03660"
     CDS             complement(4186..5442)
                     /locus_tag="VD788_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV75390.1"
                     /translation="MFDLDHWREIWNALSANKLRTCLTAFGVFWGILMLMIMLGAGNG
                     LENGVKQDFADNATNSFWVWSRSTSMPFRGLPHGRPLQLTHDDTVAIRALVPEVGVLS
                     PRTQLGGWRGGNNVTRGKNAGAFSVMGDTPEILAIEPVRLTGGRFLNRLDLADRRKVA
                     VIGPRVRQVLFGADEPPLGDHIRINGVYFKVIGTYEPINADDEEQNDSIYVPLTTFQH
                     AFNFGNRVDWLAVTSKIGIPASVAEEQVLALLRQRHRVHPDDERAFGSFNLEQEYNEV
                     QGLFTGIEALVWIVGIGTLAAGVIGVSNIMLVIVRERTHEIGVRRAIGARPWSVMGQI
                     VLEAILLTSLAGYLGLVAGVGLVELLAHWMANGAGQLEAFRHPGVAPASAIQALGLVV
                     LAGILAGLIPARRAVSVNPVDALRTE"
     gene            complement(5442..6671)
                     /locus_tag="VD788_03665"
     CDS             complement(5442..6671)
                     /locus_tag="VD788_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV75391.1"
                     /translation="MFDLDKWQEILDTIAKNRLRTFLTGFSVAWGIFMLIVLLGSGQG
                     LSNGIHHQFRDDAVNSIWIRPGQTSVPHKGLQPGRQVQFTNDDHAEIAGAVDGVEHVT
                     SRFYLDGQTMASYDDQYGNYNVRSVHPGHRYLENTLMTRGRYLNRADIEQYRKSAVIG
                     ARVEAHLFKGRPAIGEYIKLNGIAFKVVGVFDDEGPESESETIYIPISTAQRTFNGAN
                     RVHQIMLTTGTAGLDETRAMAEEITRRLAQRHRFSIEDRRAVFVANINERFAEVMGLI
                     AGIRIFVWVVGIGTILAGVVGVSNIMMIVVRERTKEIGVRKALGATPWSIVSLVLQES
                     IFITSIAGYTGLVLGVAVLELVSGKLNADFFRNPEVQLSVAVYATLLLILAGAAAGLV
                     PAIKAARVQPIQALRDE"
     gene            complement(6664..7368)
                     /locus_tag="VD788_03670"
     CDS             complement(6664..7368)
                     /locus_tag="VD788_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV75392.1"
                     /translation="MLKLTGIHKSYKIGKNELPVLRGIDLEVDAGELVSVMGSSGSGK
                     STLLNIVGLLDRHDRGEYRLDGRLIAELNESRAARYRSELIGFVFQSFNLIPFKTAAE
                     NVALPLYYQKVGRRKRNKLAREYLERVGLLDWADHHPGELSGGQQQRVAIARALISNP
                     KLILADEPTGALDTQTSYEVVETLRSINEQGVTVVIVTHEHDISEMTRRVIHLKDGSI
                     ASDRVNGRQALQAGHV"
     gene            7653..8957
                     /locus_tag="VD788_03675"
     CDS             7653..8957
                     /locus_tag="VD788_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXV75393.1"
                     /translation="MSTDPTPIAAADTGWIIVACAMVLLMTPGLAFFYGGLVRSKNAL
                     NTMMMSFAALGVVGAVWAVAGYSLAFAPGGAWVGGTDHLFLRGVDLEPRGTIPHLLFM
                     AYQGTFAVITAALISGAVVERMRFVPYLAFIGLWTLVVYAPVCHWVWGGGWLARLGAL
                     DFAGGTVVHVNAGAAAVVAALLLGTRKDYGKQALLPHNVPFALLGSALLWFGWIGFNG
                     GSALAANGTAALAAVNTLLAPAATLVVWMLLDHRRSGRPTAVGCATGIVVGLVAITPA
                     AGFVGPLGALALGALAAIPSHYCIVWRSRTRLDDALDVAAAHGVGGLVGAMLTGVLAR
                     TLWGGSAGLLDGNAAQLPIQAAGVLATTAYSAAATLGVLRAIAWVARLRVDGAREGLG
                     QDIVEHGEEAYGDGEGAILVLPESVGPRPAGTTVAQPVGGRV"
     gene            8954..9319
                     /locus_tag="VD788_03680"
     CDS             8954..9319
                     /locus_tag="VD788_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXV75394.1"
                     /translation="MKLIVAIIRPEQLHRVLESLFLHDVRGLTISRARGHGGETEQVE
                     TYRGTTVKMELVDKVRLEIGVSEPFVEVTVRAIVEAARTGEVGDGKVFVMPVERVYRI
                     RTGEQDIAAVTPAEKMTAR"
     gene            9407..10510
                     /locus_tag="VD788_03685"
     CDS             9407..10510
                     /locus_tag="VD788_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV75395.1"
                     /translation="MVLTLRFTVFRTEPVPVTVFRVARGLVEDTVTNSEAGTIRTRKR
                     AMLSPEVGGRVAALPVREGETVAAGQVLLRLADADYRARVALAERAAETSRAARRQAC
                     LAAEQAARDYERNQRLLAEQIVSEELLDRLRSERDVAVAACDAAEARVLEAEAALELA
                     GVELAKTVLRAPFAGAVAEVSTEVGEWVTPSPPALPVPPVIELIAHSSIYLSAQLDEV
                     DVAKVEVGQPVRITIDAYPDRDFSGTVTRVAPYVVDIEQHSRTFEIEVEFDDAEFAAR
                     LLPGTSADVEVILDTKPDVLRVPTYALIDGRRVLLVEDGRLVSRDVTTGLRNWSFTEI
                     ERGLAPGEPVVVSLDRAEVKSGARAVISGETLR"
     gene            10507..11211
                     /locus_tag="VD788_03690"
     CDS             10507..11211
                     /locus_tag="VD788_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV75396.1"
                     /translation="MIELRGLSRTFRVGGRPVEALRELDLTIPGGDYLSIMGPSGSGK
                     STLLNLIGCLDRPTAGSYRLDGREVSGLDEAELTQVRRHKIGFVFQSFQLIPRMTAAG
                     NVELPMTFAGVERAERRRRAGQVLTAVGLAPRSDHRPDQLSGGEQQRVAIARAIAMDP
                     AIVLADEPTGNLDSRSGREIIELLERMNQDGRTLILVTHDPEIARRAAHRIRLTDGRL
                     VEERRSTPAAGRADGG"
     gene            11211..12413
                     /locus_tag="VD788_03695"
     CDS             11211..12413
                     /locus_tag="VD788_03695"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV75397.1"
                     /translation="MGILELLRFSANALRGHRLRTTLCLLGVAIGVAAVILLTSLGEG
                     ARLYVAGEFDRLGTNLLIITPGKTETSGTAPIFGGVPHPLTVADAVAVARHVPSIAGV
                     APLSLGQASARYRDRTREVNVAGSTADLRGVRRLEIRLGSYLPAGRAHHARRLCVIGA
                     GVQRELFPGVNPLGEYLHIGEKRFQVIGVMAPRGTSLGLDLDEVVHVPVESGLRMFNQ
                     SSLFRIFAEVRSHQEIDSAKRQVIELLRERHGVEDVTVVTQDAVLETFGSIMRTLTAA
                     LAGIAAISLSVAGIGIMNVMLVSVTERTAEVGLLKALGASRRQIVGCFLVEATLISSA
                     GGAVGLIVSLTINRLLTALYPLFPVGPPDWAAWGAVVVSVLVGVVFGALPARRAAALD
                     PVAALARR"
     gene            12416..13630
                     /locus_tag="VD788_03700"
     CDS             12416..13630
                     /locus_tag="VD788_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV75398.1"
                     /translation="MARPAFRGELLGLAWGAIVAHRLRSSLTTLGISIGVAAVILLTS
                     IGEGTRRFVLDEFAQFGTNIIAINPGNTKTSGAPGALAGTVRKLTLADAEAVARIPGV
                     EHVVPVVMGMARVEREERGRSVLVYGVSSELPAVWKIGVRQGAFLPEIDLARGAPLVA
                     LGPTLKREIFADDNALGQYVRIGGFRFQVIGVMEPKGLILGWDMDDSAYIPVASAQRV
                     FNRDGLIEIDLSFAEHLAAGSIARELERVLIARHGGEEDFTVTTQTEMLDVLDRVMAV
                     VSLAVGGIGAISLVVGAIGILTMMWISVSERTTEIGLVRAIGAGAGQVLALFLLEAAL
                     LSLVGGVLGVAVGIGLARAIRLALPGFPVYTPALYVVVALLLSLAVGLASGALPARRA
                     AALDPVRALRAE"
     gene            13677..16268
                     /locus_tag="VD788_03705"
     CDS             13677..16268
                     /locus_tag="VD788_03705"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXV75399.1"
                     /translation="MRQHETVLWPNLLFVLSGAAALVLEAVFLRQLAWLFGNSAAATA
                     LVLAAFMAGLAIGSLAFGGLADRSPRPLRVFALLELGTAGSAALLAWLLGEGREWFLA
                     PIRAVGSEAARSFVEFLLAFALLLIPTTLMGGTLPALSRFVIRRMDGFVGSLGLLYGA
                     NTLGAAAGVFVAGFYLFEYAGISASAYLAASAQVCVGLTALLLSRTLGAAGAPPATPT
                     PSPTPTPAAFDRGTRAACLLAATAGGLAVLGYEVTWTRLLTLPMRSFSYSFSLMLSLF
                     LLGLCIGAVGVWLVGPRLDRPIRWVGWLQLAMGLYVAASVFWMPALLSPLGASTFERF
                     LGLSAIRAAAIVLPPTVLSGMVLPIAARGFAREAGRVGGDVGVVYALNTAGAIAGSLF
                     AGLVLLPTVGAPRTLAVLALANGSAGAIVVTQQFGRRAWQTWTAATLAAVALLPLAAA
                     GSGRYVEAFLAASRGSSGIAELLFFHEGATDTVAIVRKDYGFHDPQAKSLITNGIAMS
                     ATVKPVWRYMSVEGHLPLLFAAEPRRALAVGVGTGITLDALVSHPALERIVAVELSEG
                     VMRGLKYFEQENSRAFDDPRVELIREDGRHYLELTESRFDVITLEPPPPIVAGSVHLY
                     SLDFYRLCLARLTAGGVVAQWLPLHAQSLASARMVAATFVEAFPHAMLWLPSVRDAVL
                     IGARRPLGLDLDRLQAAYGTPGTRKNLEAAYLETPEALLATYLLDRAGIEQWAAGASV
                     ITDDRPLMEFFRHQGANMTDADTTTLLEPPQAGWDWLRDAASDPGLLDRIEDENRAMR
                     QYVRAAALDDQGLRVAAARRSRATEFHLYGLGCASAQLDFLRASSGAPAERVETHVQR
                     CAALSER"
     gene            16265..17578
                     /locus_tag="VD788_03710"
     CDS             16265..17578
                     /locus_tag="VD788_03710"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HXV75400.1"
                     /translation="MSRDEPPASALRRLGRLGGLIGRVGVSVAASRLLELGQAEPARR
                     TRRSENLVRNATRIVQTLGEMKGAAMKLGQMLSLHDGLLPPEVAQVLRSLQREAPKVP
                     AEVMQYELRGALPAFDQLFESIEPEAFAAASIGQVHRGRLRDGRRVAVKIQYPLIDEI
                     IKADLKNLRVVLQSLFRLFSDADFEPVWREVRERLLEELDYTIEADHAKRVAQLYAGD
                     PQIVVPRVIDEATATRILTMERIEGITPAEACSERHDQPARDRWGLALFDFVLSGLLE
                     HRFLHADPNLANFAFREDGRIIVYDFGCMKRVPVALARGYARLMRAAVDRRPDEIPAI
                     LHDIGVMREDGRPIERELIAPYFDLFAEIVRPAPPYAFGENERLYERLVELGISHWTE
                     AADIRFPEDIVFIDRALSGHFGNLTRLGASGPWRERVLHHTAAIG"
     gene            17681..>18228
                     /gene="pdxH"
                     /locus_tag="VD788_03715"
     CDS             17681..>18228
                     /gene="pdxH"
                     /locus_tag="VD788_03715"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HXV75401.1"
                     /translation="MNDGIAPRLDPLDRLAAWCDEARLAEVPLYDGVCLATATADGRP
                     SARMVLFKGIDRGRLLFYTDYRSRKARELDANPRAAMVFHWKTLGRQLRVEGAVERTD
                     AELSDRYFLTRGRGSRISAWTSRQSSEVESRAELERRRAEVERRFRGREVTRPEHWGG
                     YALRPVTIEFWTERPDRFHERAL"
BASE COUNT         2612 a         6232 c         6359 g         3025 t
ORIGIN      
        1 ggcgggggcc gccgtataac acccggcccg cccccccccc cgggggcccg ccgggggggg
       61 ccccccccgc ggcgggggcc gccccgcggc cgctctcgtc acaggccgac gcgagcgcga
      121 gcgccgccag caggaagatc gccgaccacc ccgaccgcgc catcgaccgc tccatagagc
      181 gacgagacta ccacgcgcgc cgtgcgctca ccgacacgcc tcacaacggc agcttgatct
      241 tgatcgccac caacctgccg ccgacgtcga cgcctccgcg cgacagctcg tcgaagtcgg
      301 cggtggcgaa gatgttgcgg aactcgacgc cgatgcggct gcgccgagtg tagacgaagt
      361 cgataccgac gaggatctgc gtggagaggt cgaacgagga gcggctggtc gccgtttccg
      421 accagatgac cgacgacggc acctcgagct ccgagatcat cagacccaag ccgaagccga
      481 cgtagccttc gaacagccag aagggcacga tcgcctggac gttgcccgtc aggaccagat
      541 tggtcaggaa catctcgtcg tcggcgaact gcagttcggt cggggtgtcg taactgcggc
      601 cggcgaacaa cgaatcgaag gcgaaggcca ggtggcgggc atgccgccga ccgactccgc
      661 cgccgatcgc gaagcccgcg tcgctgccgc gcaagccgtc gcccgtgcgg ccgtcgaaat
      721 agccgaggtt gccgaagctg tactgccatc cgtccgcgtg gtggaccgcg ttctgggcgg
      781 cagcgccggg agccgcgaac cacaccgccg acaacagcaa ccccgccgcc ctgcgcccgt
      841 tcgtcaagcg ttccttccgt ccggggcggc cggtgcgctc cgtcgaatcg ttccgacacg
      901 cgtgccgggc gccgcgcaat gagtgtaaac tgtgcggttc atgcgcgcga aagcagccga
      961 gccgctcgcg gccgagcgag tcgatcccgc cgaacgccgc ctggctgtcg ccctacggct
     1021 cggcggatcg ctgctgttgc tcgccttcgg cgccgtgctg cttccgacgc agtcgatgac
     1081 ggcgacccat cgctggctga cgatgggtga gtttcccgaa tcgccgctgg ccgactacct
     1141 cacccgctcg atctcgttgc tctacggcat caagggcggc atgtactggg tgctcgccac
     1201 cgacgtccgg cgctacgccc ccgtcatccg ctacgtcggt tggacgacga tcggtttcgg
     1261 cgtgctgatg gtcgcgatcg actaccgggc cggcatgccg ctgttctgga cgttgagcga
     1321 ggggccgccg atcgcggcga tcggcatgct gttcctgctg ctgctgcgcc gcgtcgacgc
     1381 ggtcgggcgc gcgtgaccgc ggtccccggc gatcaccgta taattagcgg gagccgaggg
     1441 ggtcatgacg gacgatgccg acaggccggc agccgacgaa cggccggcct cccgactgat
     1501 cgaaacgctg agcgcggtgc tgggaaacct ggctcgaccc tcggccgtgc tcaagacgat
     1561 cctgcaccag gcggtggcgc aaacgagcgc cgagcgtggc gtcttcgtcg aggtcgggcg
     1621 ctcgggccgg atgacctatc gcgtgctcta ccgcttcgag cgggaagagc tggcggggcc
     1681 ggccggctcc ttcagtgcga cgatcttcgc gcgagtgctc gaaaccggcg aggggatcgt
     1741 gctgggcaac gcgatggaag actcgcgctt cgacggctcc gggtcgatcc acgacttgaa
     1801 cctcgtatcg gtcatgtcgc tgccgattcg cgtcggcggc gagatcgcgg cgctggtcca
     1861 cctcgagcat acgcgaccgg accactttac cgagcggcat cgccggctcg tcgaatcgct
     1921 gctcgagctg tcgggtccgg tgctggcggc gctgcgggcc ggcgcggacg tgatccagca
     1981 gaacgacgcg ttgcgtcaga ccgcgtcgcg cgcgcgcgag cacctcgagt ccgaccgcga
     2041 ccaactgacg cgcgactgga gcttcggccg cttcgtcggc cgctcggcgg cggtgcgcgc
     2101 gctcggtcac tcggtggcca aggcggcgcg gtccgagtcg ccggtgttgt tgaccggaga
     2161 gaccggaacg ggcaagagca tcctggctcg cgtgctgcac caccagagcc ggcggcgcaa
     2221 tgccgcgatg gtgaccgtgt tctgcccgtc gctcgaacgc ggcatggtcg aggccgagct
     2281 gttcggccac cgacgcgggg cgttcaccgg cgccgtcgcc gatcgcgtcg gcaaggtgca
     2341 ggccgcggag gacggcacgc tgttcctcga cgagatcggc gagcttcccc tcgagatcca
     2401 accgaagctg ttgcgcttgc tgcaggagaa gacctacgag cgcgtcggcg atgccgtcga
     2461 gcgcatcgcg aacgtcaggg tcatcgcggc gaccaaccgt gacatccgcc gcgaggtgca
     2521 gatcggccgc ttccgccgcg atctgtacga acggctcaac ttcgtgccga ttcgcgtccc
     2581 accgttgcgc gatcgccggt acgacatccc ggtgctgctg cgtcactgtc tggatcagca
     2641 cgaccagggc cgctggatcg aggtgtcgcc cgatgccgag cgctatctga tggagctcga
     2701 tttcgcctgg cccggcaacg tacgacacgt cgagcagatg gcggcgcggc tggcgctcga
     2761 gcggttcgtc gaaccggtca ccgtcggcga tctggaatcc gtcatcgacg cggatcggga
     2821 agcggccgac gacggcgaag ggagtcggcc ctccgaacga gagctggagg ccgggctgcc
     2881 cgcctttctc gcgcatgccg agaagcgctg gctcgtcgag gcgctgcgcc gctattccga
     2941 cctgtcgaag aaggagctgg cgcgcaagct gaacatcggc gagagcacgc tacacaagaa
     3001 gatccggcag tacggcatct cggcctgatc ggccgcgcgc ccggacgcct gtggttcgcg
     3061 gcgtcagccg gccgagttcg ggtccttgac gcggtcgtcc gcggtcaggc cgccgaccac
     3121 ctcgatctgg atcccgtcgg acagcccggt ctcgatctcg cgccgttcga actgctggtc
     3181 ggccgtttcg acttcgacgt aggtcacgtc gccgtcgaac tgcagcaggc cctcgctgat
     3241 cgcgagcacc tcgtcccgac ggtcgagcac gatatcggcg ttggcgctgt agttggcgcg
     3301 cacgaacgcg ccgtcctgct gtgccagcgc ggcgcggatc tcgaactgga tcgcgccgtt
     3361 ctcctcgacg cccttgggcg cgatgtgctc gagcgtcgcc ttgaaggtct taccctcgat
     3421 cgcgccgatc gtcagcagca gctccattcc cggtcggatc ttgccgacct ccgattcgtc
     3481 gaccagtccc tcgaagatca gctcgtccat gtcggcgatg gtggcgatcg tcgtgccctc
     3541 gttgaaggtg ttggcctcga tcaccgagtc tccgacctcg accggcacct cgagcaccat
     3601 cccctcgatc gtcgcctcga ccagggtgtt ggtcgattct ccggccttgg cgcgggtgcc
     3661 cttcttgatc agctcgaggt tgtcgaccga cgccgccagc tcttccttgg cactgtcgta
     3721 ggccagctcg aactgctgca gctcggtgcg tgcgaccacg ccctcggagg ccagccgccg
     3781 gctgcgctcg atgtcgatct cggcgttctc caggttgatc cgcgcgcggt tgacccgcgt
     3841 ttccgcgttg gccaggttga ccatgtcggg gatgatgcgg atccgcgcga tccgagcgcc
     3901 ggcctcgact cggtcgccgg cctcgacgaa caactcgtcg accaccccgg acacctgcgg
     3961 cttgatctcg acctccttgc gcgggacgac cgagccggtg gcgaccgtct tgtagacgat
     4021 gtcggtcacc gacgggcttt ccgtccggta ggtcaccggc tgttcctggg acttgctgta
     4081 caggtagaag atcgtgcccg cgaacaacga cagcgcgacg aggccgatca ggatcgtgac
     4141 gactttcttc atgacggtca tggctccaat cgttgtgccc gatgatcatt cggtgcgcag
     4201 cgcgtcgacc gggttgaccg agacggcccg tcgtgccggg atgaggccgg cgaggatccc
     4261 ggccagcacg accaggccga gcgcctggat cgcactggcc ggcgccacgc cggggtggcg
     4321 aaacgcctcc agctggccgg cgccgttggc catccagtgg gccagtaact cgaccaggcc
     4381 gaccccggcg acgaggccca ggtaaccggc gagcgaggtc agcaggatcg cctccagcac
     4441 gatctggccc atcacgctcc acggccgcgc accgatcgcc cggcgcacac cgatctcgtg
     4501 cgtccgctcg cgaacgatga cgagcatgat gttgctgacg ccgatcaccc cggcggccag
     4561 cgtgccgatg ccgacgatcc agaccagcgc ttcgatcccg gtgaacaacc cctggacctc
     4621 gttgtactcc tgttccaggt tgaagctgcc gaacgcgcgc tcgtcgtccg ggtggacgcg
     4681 gtgccgctgg cgcaacaacg ccagcacctg ctcctcggcg accgaggccg ggatgccgat
     4741 cttcgaggtg acggcgagcc agtcgacgcg gttgccgaag ttgaacgcgt gctggaacgt
     4801 cgtcagcggc acgtagatcg aatcgttctg ctcctcgtcg tcggcgttga tcggctcgta
     4861 ggtcccgatc accttgaaat agacaccgtt gatccggata tggtcgccga gcggaggttc
     4921 atcggcgccg aacagcacct ggcgcacgcg cgggccgatc accgcgacct tgcgccggtc
     4981 ggccaggtcc agccggttga ggaagcgacc gccggtgaga cgcacgggct cgatcgccag
     5041 aatctcgggc gtgtcgccca tcacgctgaa cgcgccggcg tttttgccgc gcgtcacgtt
     5101 gttccccccg cgccaaccgc cgagttgggt gcgcggcgac agcacgccga cctccggcac
     5161 gagggcccgt atcgccaccg tgtcatcgtg ggtcagctgc aacggccgcc cgtgcgggag
     5221 accgcgaaac ggcatgctcg tcgagcgcga ccagacccag aagctgttgg tcgcgttgtc
     5281 ggcgaagtcc tgcttgacgc cgttctcgag gccgtttccc gcgccgagca tgatcatcag
     5341 catcaggatg ccccagaaca cgccgaacgc cgtcagacag gtgcgcagct tgttcgcgct
     5401 caacgcgttc cagatctcgc gccagtggtc gagatcgaac atcactcgtc cctcagcgcc
     5461 tggatcggtt ggacccgcgc ggctttgatc gccggcacca agccggcggc ggcgccggcg
     5521 aggatcaaca acaacgtggc gtagacggcg acgctcagct ggacctccgg gttgcggaag
     5581 aaatcggcat tgagcttgcc ggaaaccagc tcgagcacgg cgacgcccaa caccagcccg
     5641 gtgtatccgg cgatcgacgt gatgaagatc gactcctgca ggaccaggct gacgatcgac
     5701 cacggcgtcg cgccgagcgc cttgcgcacg ccgatctcct tggtgcgctc gcgcaccacg
     5761 atcatcatga tgttgctgac gccgaccacg ccggcgagga tcgtaccgat gccaacgacc
     5821 cagacgaaga tacgaatgcc cgcgatcagc cccatcacct cggcgaagcg ctcattgatg
     5881 ttggcgacga agacggcccg ccggtcttcg atcgagaagc ggtggcgctg ggccagccgt
     5941 cgggtgatct cttcggccat cgcccgcgtt tcgtccaagc ccgcggtgcc ggtggtcagc
     6001 atgatctggt gcacgcggtt cgcgccgttg aacgtgcgct gcgccgtcga gatcgggatg
     6061 taaatcgtct ccgactcgga ctccggcccc tcgtcgtcga agacgccgac gaccttgaac
     6121 gcgatgccgt tcagcttgat gtactcgccg atcgccggcc ggcccttgaa caggtgggct
     6181 tcgacgcgtg cgccgatcac ggccgacttg cggtactgtt cgatgtcggc ccggttgagg
     6241 taccgcccgc gggtcatcag cgtgttctcg aggtaccgat ggcccgggtg gaccgatctg
     6301 acgttgtagt tgccgtactg gtcgtcgtag gacgccatcg tctggccgtc gaggtagaac
     6361 cgcgaggtga cgtgctcgac gccgtcgacc gcgccggcga tctcggcatg atcgtcgttg
     6421 gtgaactgca cctgacggcc gggctgcagc cccttgtgcg gcacgctggt ttggccgggc
     6481 cggatccaga tgctgttgac cgcgtcgtcg cggaactggt ggtggatgcc gttgctcagc
     6541 ccctgaccgg aaccgagcag cacgatcagc atgaagatgc cccaggcgac gctgaagccg
     6601 gtgaggaacg tcctcagccg gttcttggcg atcgtgtcga ggatctcctg ccacttgtcg
     6661 agatcaaaca tgacccgcct ggagcgcctg tcggccgttg acgcggtccg acgcgatcga
     6721 tccgtctttc aggtggatca cccgccgtgt catctcggag atgtcgtgct cgtgggtcac
     6781 gatcaccacc gtcacgccct gctcgttgat cgatctcagc gtctcgacga cctcgtagga
     6841 ggtctgggtg tcgagcgcgc cggtcggctc atcggccagg atcagcttgg ggttcgaaat
     6901 cagcgcacgc gcgatcgcga cgcgctgctg ttgaccgccc gacagctcgc cggggtgatg
     6961 atcggcccag tcgagcaggc cgacgcgctc gaggtactcg cgcgccagct tgttgcgttt
     7021 gcgacggccg accttctggt agtacagcgg cagcgccacg ttctcggcgg cggtcttgaa
     7081 cgggatcagg ttgaacgact ggaagacgaa gccgatcagc tcgctgcgat agcgggccgc
     7141 gcgcgactcg ttcagctcgg cgatcaagcg accgtccaag cggtactcgc cgcggtcgtg
     7201 acgatcgagc agaccgacga tgttgagcag cgtcgacttg cccgatcccg acgagcccat
     7261 caccgacacg agctcgcccg cgtcgacttc gagatcgatg ccgcgcagca ccggcagctc
     7321 gttcttcccg atcttgtacg acttgtggat gcccgtcagc ttcagcatct gcccttccgc
     7381 cgacgcctgg ccgcgccgcc ccggtctctg ggtataacgt cctgggcgcg cgatggtttc
     7441 ctccggtccg tccggcatct ccgccgcccg ggggacgggg ccctcaccgg tcgcttccgg
     7501 tgggagtcca ggatactggt gaccaaaaaa cagtgctgaa tctacacaaa actacaaccg
     7561 actgtggaaa ttgatcttct tctcggtaaa ttgatcacga gaaataaacc gatatcacaa
     7621 ttttctaaga tcatctccga ggagaaggcc ccatgtcgac cgatccgacc ccgatcgcgg
     7681 cggccgacac cggctggatc atcgtcgctt gcgcgatggt cctgctgatg acgcccggcc
     7741 tggccttctt ctacggcggg ctggttcgct cgaagaacgc gctgaacacc atgatgatga
     7801 gcttcgccgc gctcggtgtg gtcggtgcgg tgtgggccgt cgccggatac agcctggcgt
     7861 tcgctccggg cggggcctgg gtcggcggta cggaccatct gttcctgcgc ggtgtcgacc
     7921 tcgagccgcg cggcacgatt ccgcacctgc tgttcatggc gtaccagggc accttcgccg
     7981 tcatcaccgc ggcgctgatc tcgggcgcgg tcgtcgagcg catgcggttc gtgccctacc
     8041 tggcgttcat cgggctctgg acactcgtcg tctacgcgcc ggtctgccac tgggtctggg
     8101 gcggaggctg gctggcccgg ctgggcgcgc tcgatttcgc cggcggcacc gtggtccacg
     8161 tcaacgccgg cgccgcggcc gtggtggcgg cgctgctgct cggcacgcgc aaggactacg
     8221 gcaagcaggc gttgctgccc cataacgtac cgttcgcgct gctcgggtcg gcgctcttgt
     8281 ggttcggttg gatcggattc aacgggggca gcgcgctcgc ggccaacggg acggcggcgc
     8341 tggccgcggt caacacgctg ctggcgccgg cggcgaccct cgtcgtgtgg atgttgctcg
     8401 atcaccgccg cagcggccgg ccgacggcgg tgggctgcgc caccggcatc gtggtcggcc
     8461 tggtcgcgat caccccggcc gccggcttcg tcggtccgct gggtgcgctg gcgctgggtg
     8521 cgctcgcggc gattcccagc cattactgca tcgtctggcg cagccggacc cgcctggacg
     8581 acgcgctcga cgtcgccgcg gcccacggcg tcggcgggct ggtcggcgcg atgctgaccg
     8641 gcgtattggc gcgtacgctg tggggcgggt cggcgggcct gctcgacggc aacgccgctc
     8701 agctgccgat ccaagcggcg ggcgtgctgg ccacgacggc ctacagcgcg gccgccacgc
     8761 tcggcgtgct gcgtgcgatc gcctgggtcg cgcgcttgcg cgtcgacggc gcgcgcgagg
     8821 ggctgggcca ggacatcgtc gagcacggcg aggaagccta cggcgacggc gagggagcga
     8881 tcctggtcct gcccgagtcg gtcggcccac gaccggccgg tacgacggtc gcgcagccgg
     8941 tcggaggtcg ggtatgaagc tgatcgtcgc gatcatccgg cccgagcagc tccaccgcgt
     9001 gctcgagtcg ctgttccttc acgacgtgcg cgggctgacg atcagccgtg cccggggcca
     9061 cggcggcgag accgaacagg tcgaaaccta ccgcgggacg acggtcaaga tggagctggt
     9121 cgacaaggtg cggctcgaga tcggggtgtc ggagccgttc gtcgaggtca ccgtgcgtgc
     9181 gatcgtcgag gccgcgcgca ccggcgaggt cggcgacggg aaggtcttcg tgatgccggt
     9241 ggagagggtc tatcgcatcc gcaccggcga gcaggacatc gctgcggtga cgccggccga
     9301 aaagatgacc gcccggtgag acggccggcg gccgggctgc gctaacatcg gttcgatggc
     9361 gtcccggcac cttcgagtgg cggggtcggt cgcggcgatc gtcgccgtgg tactcaccct
     9421 gcgtttcacc gtctttcgca ccgagcccgt cccggtgacc gtcttccgcg tcgcccgcgg
     9481 tctggtcgag gacacggtga ccaacagcga ggccgggacg attcgcacga gaaagcgggc
     9541 gatgctcagc ccggaagtcg gcgggcgggt ggccgcgctc ccggtgcgcg agggggaaac
     9601 cgtcgccgcc ggccaggtgc tgcttcgcct tgccgacgcc gactaccgag cgcgggtcgc
     9661 actcgccgag cgggcggccg agacgtcgcg cgccgcgcgg cgccaagcct gcctcgcggc
     9721 ggagcaggct gcccgcgatt acgagcgcaa ccaacggttg ttggccgagc agatcgtgtc
     9781 ggaggagctg ctcgaccggc tgcgcagcga gcgcgacgtg gccgtcgcgg cgtgcgatgc
     9841 ggcggaggcg cgcgtgctcg aggccgaggc ggcgctcgag ctggccggcg tcgagctggc
     9901 gaagacggtg ctgcgcgcac cgtttgccgg tgcggtcgcc gaggtgtcga cggaggtcgg
     9961 cgagtgggtc actccttctc cgcccgcact tcccgtgccc ccggtcatcg agctgatcgc
    10021 acactcgtcg atctacctca gcgcgcagct cgacgaggtc gacgtggcca aggtcgaagt
    10081 cggccaaccg gttcggatca cgatcgacgc ctaccccgac cgcgacttct ccgggacggt
    10141 cacgcgcgtc gcgccgtacg tcgtcgacat cgaacagcac agccggacct tcgagatcga
    10201 agtggagttc gacgacgccg agttcgccgc ccggctgttg ccgggaacct cggccgacgt
    10261 cgaggtgatc ctcgacacca agcccgacgt gctgcgcgtg ccgacctacg cgctgatcga
    10321 cggcagacgc gtgctgctcg tcgaggacgg caggctcgtt tcgcgcgacg tgacgacggg
    10381 gctgcgcaac tggtcgttca ccgagatcga gcggggtctg gcgccgggcg agccggtcgt
    10441 cgtctcgctc gaccgcgccg aggtcaaatc gggcgctcgc gccgtgatct cgggcgagac
    10501 gctgaggtga tcgagctgcg cggcctatcg cgcaccttcc gcgtgggcgg acgaccggtc
    10561 gaagcgctgc gcgagctcga tctgacgatc cccggcggcg actacctgtc gatcatgggg
    10621 ccgtcgggat cgggtaagtc gacgttgctc aacctgatcg gttgtctcga ccgaccgacc
    10681 gccggatcct accgcctcga cggccgggag gtgtccggtc tcgacgaagc cgagttgacg
    10741 caggtccgcc ggcacaagat cggtttcgtc ttccagtcgt tccagctgat cccgcgaatg
    10801 accgcggcgg gcaacgtcga attgccgatg accttcgccg gggtcgagcg cgccgagcga
    10861 cggcgccggg ccggccaggt gctgaccgcc gtcgggctgg caccgcgcag cgaccatcga
    10921 ccggatcagc tgtccggcgg cgagcagcaa cgggtggcga tcgcacgcgc gatcgcgatg
    10981 gacccggcga tcgtgctggc cgacgaaccg accggcaacc tcgacagccg ctcgggccgg
    11041 gagatcatcg agctgctcga acgaatgaac caggacggcc gcacgctgat cctcgtcacg
    11101 cacgatccgg agatcgcccg gcgcgcggcc catcgcatcc ggctgaccga tggccgtctc
    11161 gtcgaggagc gacgatcgac gccggcggcc ggccgcgcgg acggtggctg atgggaatac
    11221 tcgagctgct gcgcttttcg gccaacgcgc tacgcgggca ccggctgcgt acgacgttgt
    11281 gcctgctcgg ggtcgcgatc ggcgttgcgg cggtgatcct gttgaccagt ctgggcgagg
    11341 gagcgcggct gtacgtcgcc ggcgagttcg accggctcgg caccaacctg ttgatcatca
    11401 caccgggcaa gaccgagacc agcggaaccg cgccgatctt cggcggcgtg ccgcacccgc
    11461 tgaccgtggc ggatgccgtg gccgtggcgc gccacgtccc ctcgatcgcc ggcgtcgccc
    11521 cgctgtcact cggtcaggcc agcgcgcgct accgcgatcg cacgcgcgag gtcaacgtcg
    11581 ccggctcgac cgccgacctg cgcggcgtgc gtcggctcga gatccgtctc ggtagctacc
    11641 tgcccgccgg gcgcgcgcac catgcgcgac ggctgtgcgt gatcggtgcc ggggtccagc
    11701 gcgagctgtt tcccggcgtc aacccgctcg gcgagtacct gcacatcggt gagaagcgct
    11761 tccaggtgat cggcgtgatg gccccgcggg gtacctcgtt ggggctcgat ctggacgagg
    11821 tggtccacgt cccggtcgaa agcggcctgc gcatgttcaa ccagtcgagc ctgttccgca
    11881 tcttcgccga ggtgcgcagc caccaggaga tcgatagcgc gaaacgccag gtgatcgagc
    11941 tgctgcgcga gcggcacggt gtcgaggacg tcaccgtcgt gacccaggat gcggtgctcg
    12001 agaccttcgg cagcatcatg agaaccctga ccgccgcact cgcgggcatc gccgccatct
    12061 cgctgtcggt cgccggcatc ggcatcatga acgtgatgct ggtatcggtc accgagcgca
    12121 cggcggaggt cggtctgctc aaggcgctcg gcgccagccg gcggcagatc gtcggctgct
    12181 tcctcgtcga ggcgacgctg atatcctcgg ccggcggcgc cgtcggcctg atcgtttcgc
    12241 tgacgatcaa ccgcttgctc accgcgctct acccgctgtt tcccgtgggc ccgcccgact
    12301 gggccgcctg gggcgccgtc gtcgtatcgg tcctcgtcgg cgtcgtcttc ggcgcgttgc
    12361 cggcccgacg cgcagccgcg ctcgacccgg tcgccgcgct ggcgcggagg tgacggtggc
    12421 ccggcctgcg ttccgcggcg agctgctcgg tctggcctgg ggcgcgatcg tggcccaccg
    12481 cttgcgatcg tcgctgacga cgctcggcat ctcgatcggc gtggccgcgg tgatcctgct
    12541 gacctcgatc ggcgagggca cgcgccgctt cgtgctcgac gagttcgccc agttcggcac
    12601 caacatcatc gcgatcaacc ccggcaacac caagacctcg ggcgcgccgg gcgcgctcgc
    12661 cggcacggtg cgcaaactga cgctggccga cgccgaggcg gtcgcgcgca tccccggtgt
    12721 cgagcacgtc gtgccggtgg tgatggggat ggcgcgcgtc gagcgcgagg aacgcggccg
    12781 cagcgttttg gtctacggcg tcagctccga gcttcccgcg gtctggaaga tcggtgtgcg
    12841 ccagggggcg ttcctccccg agatcgatct cgctcgcggg gccccgctgg tcgcactcgg
    12901 tccgacgctg aagcgggaga tcttcgccga cgacaacgcc ctcgggcaat acgtgcgcat
    12961 cggcggtttt cgcttccagg tgatcggcgt gatggagccg aaggggctga tcctgggctg
    13021 ggacatggac gattcggcct acatcccggt ggccagcgcg cagcgcgttt tcaaccgcga
    13081 cgggctgatc gagatcgacc tgtcgtttgc cgagcacctc gctgccgggt cgatcgcacg
    13141 cgagctcgag cgcgtgctga tcgcgcgcca cggcggcgag gaggacttca ccgtcacgac
    13201 gcagaccgaa atgctcgacg tgctcgaccg cgtgatggcc gtcgtcagcc tggccgtcgg
    13261 cggaatcggt gcgatctcgc tcgtggtcgg cgcgatcggc atcctgacga tgatgtggat
    13321 ctcggtcagc gagcgcacga cggagatcgg tttggtgcgc gcgatcggcg ccggagcggg
    13381 acaggtgctg gcgctgttcc tgctcgaggc cgcgctgctg tcgctggtcg gcggcgtgct
    13441 gggcgtcgcc gtcgggatcg gtctggcccg cgcgatccgc ctggcactcc cgggcttccc
    13501 ggtctacacg ccggcgctct acgtcgtcgt cgcgctgctg ctcagcctgg cggtcggtct
    13561 cgccagcggg gcgctgccgg cgcggcgggc ggcggcgctc gacccggtcc gcgcgttgcg
    13621 cgccgaatag ccgataatga accgcgatac agcaccggca ggaacgacaa ggctccatgc
    13681 ggcaacacga gaccgtcctc tggcccaatc tgctgttcgt gctgtcgggc gcggcggcgc
    13741 tggtcctcga ggcggtcttt ctgcggcagt tggcctggct gttcggcaac agcgccgcgg
    13801 cgacggcgct cgtgctggct gcgttcatgg ccggtctcgc catcggctcg ctggccttcg
    13861 gcggtctggc cgatcgctcg ccgcggccgc tccgcgtctt cgcgctgctc gagctgggca
    13921 cggccggttc cgctgcgctc ttggcctggc tgttggggga ggggcgcgag tggttcctcg
    13981 cgccgatccg cgccgtcggc tcggaggccg cgcgatcctt cgtcgagttc ctgctggcgt
    14041 tcgcgctcct gctgatcccg accacgctga tgggcgggac gctgccggcg ctcagtcggt
    14101 tcgtgatccg gcggatggac ggattcgtcg gatcgctcgg cctgctgtac ggcgcgaaca
    14161 cgctcggcgc cgcggcgggc gtgttcgtcg cggggttcta cctgttcgag tacgccggga
    14221 tctcggccag cgcctacctg gcggcgtcgg cccaggtttg cgtcgggctg acggccctgc
    14281 tgttgtcccg cacgctcggc gccgcgggcg ctccgccggc gacgccgaca ccgagcccga
    14341 caccgacgcc ggcagcgttc gaccgaggga cgcgcgcggc ctgtctgttg gccgcgaccg
    14401 cgggcgggct ggccgtgctg ggctacgagg tgacctggac caggttgttg accctgccga
    14461 tgcggtcgtt ctcctactcg ttcagcctga tgctctcgct gttcctgctc gggctgtgca
    14521 tcggggcggt cggcgtgtgg ctcgtcggcc cgcgtctcga ccggccgatc cgctgggtcg
    14581 gttggctcca actggcgatg ggactgtacg tcgccgcgtc ggtgttctgg atgccggcgc
    14641 tgctctcacc gctcggcgcg tcgaccttcg aacggttcct cggcctgtcg gcgatccgag
    14701 cggcggcgat cgtgctgccg ccgaccgtgc tgtccggcat ggtgttgccg atcgcggcgc
    14761 gcgggttcgc ccgggaggcg ggtcgcgtcg gcggggacgt cggcgtggtc tacgcgctga
    14821 acaccgcggg tgcgatcgcc ggttcgctct tcgccggact cgtgctgctt ccgaccgtcg
    14881 gtgccccgcg gactctcgcc gtcttggcgc tcgccaacgg aagtgccggc gcgatcgtcg
    14941 tgacccagca gttcgggcgt cgcgcgtggc agacgtggac cgccgcgacg ctcgccgcgg
    15001 tcgcgctgct gccgctcgcg gccgccggca gcggacgcta cgtcgaggcc tttctcgccg
    15061 ccagccgtgg gtcgagcggg atcgccgagc tgctcttctt ccacgagggc gcgaccgaca
    15121 cggtcgcgat cgtgcgcaag gactacggct ttcacgatcc gcaagccaag agcctgatca
    15181 cgaacggcat cgcgatgtcg gccacggtca agcccgtctg gcgctacatg tcggtcgagg
    15241 ggcatttgcc gctgctgttc gccgccgagc cgcgacgggc gctcgccgtc ggcgtcggca
    15301 ccgggatcac gctcgacgcg ctggtctccc atcccgcact cgagcgcatc gtcgccgtcg
    15361 agttgagcga gggggtgatg cgcggactga agtacttcga gcaggaaaac tcgcgggcct
    15421 tcgacgaccc gcgggtcgag ctgatccgcg aggacggtcg gcactacctc gagctgaccg
    15481 agtcgcgctt cgacgtgatc acgctcgaac caccgccgcc gatcgtggcc ggctcggtcc
    15541 acctctactc gctcgacttc tatcgcctgt gtctggcacg gctgacggcg gggggtgtcg
    15601 tcgcgcagtg gctgccgctg cacgcgcaga gcctggcgtc ggcgcggatg gtggcggcga
    15661 ccttcgtcga ggcctttccc cacgcgatgt tgtggctacc gtcggtgcgc gatgcggttc
    15721 tgatcggcgc gcgacgaccg ctcggtctcg atctcgatcg gctgcaggcc gcctacggca
    15781 cgccggggac gcgaaagaac ctcgaggcgg cctacctcga aacgccggaa gcgctgctcg
    15841 ccacctactt gctcgatcgc gccggcatcg agcaatgggc ggccggggcg agcgtgatca
    15901 ccgacgaccg gccgttgatg gagttcttcc gccaccaggg cgccaacatg accgacgccg
    15961 acacgacgac cttgctcgag ccgcctcagg ccggctggga ctggttgcgt gacgccgcat
    16021 ccgaccccgg attgctcgat cgtatcgagg acgagaaccg cgcgatgcgt caatacgtgc
    16081 gcgctgcggc gctcgacgat cagggcctgc gtgtcgccgc agcgcgccga tcgcgcgcga
    16141 ccgagttcca tctctacggc ctgggctgcg ccagcgccca gctcgacttt ctccgcgcct
    16201 cgtccggcgc gccggccgag cgggtcgaga cgcacgtgca gcgctgcgcc gcgttgagcg
    16261 agcgatgagt cgcgacgagc ccccggcttc cgcgttgagg cggctcggcc gtctgggcgg
    16321 gctgatcggt cgcgtcggcg tctcggtcgc ggcgtcccgc ttgctcgaac tgggtcaggc
    16381 cgagcccgcg cgccggacgc ggcgcagcga gaacctcgtg cgcaacgcga cgcgcatcgt
    16441 ccaaacgctc ggcgagatga agggcgcggc gatgaagctc ggacagatgc tcagtctgca
    16501 cgacgggctg ttgccgcccg aggtggcgca ggtgctgcgc tcgctgcagc gcgaggcgcc
    16561 gaaggtaccg gccgaggtca tgcaatacga gctgcgcggc gcgctgcccg cgttcgacca
    16621 gttgttcgag tcgatcgagc ccgaggcgtt cgccgccgcg tcgatcggcc aggtgcaccg
    16681 cgggcgactg cgcgacggtc gccgggtggc ggtcaagatc cagtacccgt tgatcgacga
    16741 gatcatcaag gccgatctga agaacctgcg ggtggtgctg cagtcgttgt ttcgcctgtt
    16801 ctccgacgcc gacttcgaac cggtctggcg cgaggtgcgc gagcggctgc tcgaggagct
    16861 ggattacacg atcgaggccg accacgccaa gcgcgtggcg caactgtatg ccggagaccc
    16921 gcagatcgtc gtgccacgcg tgatcgacga ggccaccgcg acgcggatac tgacgatgga
    16981 gcgcatcgaa gggatcacgc cggccgaggc ctgctccgag cgccacgatc agccggcacg
    17041 cgaccgctgg ggtctcgcgc tcttcgattt cgtgctgagc gggctgctcg agcatcgctt
    17101 cctgcacgcc gatcccaacc tggccaactt cgcctttcgc gaggacgggc ggatcatcgt
    17161 ctacgacttc ggttgcatga aacgcgtgcc ggtggcgctg gcgcgcggct acgcgcggtt
    17221 gatgcgtgcc gcggtcgacc gccggcccga cgagatcccg gccatcctcc acgacatcgg
    17281 ggtgatgcgc gaggacggcc gcccgatcga gcgcgagctg atcgccccct acttcgacct
    17341 gttcgccgag atcgtgcgcc cggcgccgcc ttacgccttc ggcgagaacg agcggctgta
    17401 cgaacggctg gtcgagctcg gcatcagcca ctggaccgaa gcggccgaca tccgcttccc
    17461 cgaagacatc gtcttcatcg atcgcgcgtt gtccggtcac ttcggcaacc tgactcgtct
    17521 cggcgccagc ggcccgtggc gcgagcgcgt actgcaccac acggcggcga tcggttgaga
    17581 cgcggtgccg gacggcggaa ggcggcgttg cgctagagtg tccggcgcaa ggagatgtcg
    17641 gcgtgtcgcg gccagcggaa ctgccccgac gggcgagacg atgaacgacg ggatcgcgcc
    17701 gcgcctcgat ccgctcgacc gtctcgcggc gtggtgcgac gaggcccggc tggcggaggt
    17761 tccgctctac gacggcgtct gtctggcgac cgcgacggcg gacggccggc cctccgcgcg
    17821 gatggtgttg ttcaaaggca tcgatcgggg acggctgttg ttctacaccg actaccgcag
    17881 tcgcaaggcg cgggagctcg acgccaaccc gcgcgcggcg atggtcttcc actggaagac
    17941 gctcggccgt caactgcgcg tcgagggagc ggtcgagcgc accgatgccg agctgtccga
    18001 tcgctacttc ctcacacgcg gccgaggcag ccgcatctcc gcctggacct cgcggcagag
    18061 ctcggaggtc gaaagccgcg ccgagctcga gcgccggcga gcggaggtcg agcgacgttt
    18121 tcgcggccgc gaggtcacgc gaccggagca ctggggcggg tacgccctcc gtccggtcac
    18181 gatcgagttc tggaccgagc gccccgaccg ctttcacgag cgcgcact
//
LOCUS       DASPGN010000134         3736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_373893, whole genome shotgun sequence.
ACCESSION   DASPGN010000134 DASPGN010000000
VERSION     DASPGN010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373893"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..2846)
                     /locus_tag="VD788_03720"
     CDS             complement(177..2846)
                     /locus_tag="VD788_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75402.1"
                     /translation="MNAEIERRRQADLGQAPWRRWGPYVSERQWGTVREDYSPGGTAW
                     DYVSHDQARSRAYRWGEDGIGGVCDEQQRICLALALWNGEDAILKERLFGLTGSEGNH
                     GEDVKELYYYLDNNPTHSYMRMLYKYPQSAFPYARLVDENRRRGKHEGEFELVDTGIF
                     DDGRYFDVFIEYAKAGPDELLLRVTAHNRSETAARLELLPQIWFRNTWRWGRSAERPR
                     LAAVDDSTIEVDCPGLGEFFLHADGAPELLFCENETNSKRLWGVERPGYFKDGIHEYV
                     INGRAEAVDPARQGTKAAARFVRMVAAGGSTTVQLRLSPERRRAPFRDADAVFEARRR
                     ETDEFYRALQSGIEGDEARSIQRQALAGMLWSKQFYHFDVGQWLEGDPGQPSPPAERR
                     RGRNAEWTHLNNADVVSMPDKWEYPWYAAWDLAFHCVPLALVDPEFAKEQLVLLTREW
                     YMHPNGQLPAYEWAFGDVNPPVHAWGAWRVYEIDRNQRGGKGDLRFLERVFHKLLLNF
                     TWWVNRKDVNGRNVFQGGFLGLDNIGVFDRSTELPTGGHIDQADGTSWMAMYSLNLMR
                     IALELARENEVYEDVATKFFEHFLYIAAAMNNVGGDGIELWDPDDEFYYDVLHTPDGR
                     SSRMKVRSMVGLIPLFAVEVLEPELLEAVPGFRRRLEWFLDYRPDLAELVSRWQVPGR
                     GEQRLLSLLRGHRMKRLLARMLDPGEFLSDYGVRALSRHHLAHPFELDWSGQRFTVDY
                     QPGESNSGLFGGNSNWRGPIWFPVNGLIVHSLRRFHSYYGDQFRVECPTGSGRFVSLA
                     EVADEISRRLTSIFTRDERGRRPVLGDQPLLQSDPHFRDHVPFYEYFHGDTGRGVGAG
                     HQTGWTGLVAELLRAPAAVPQATAGSLAVEEKV"
     gene            complement(2843..>3736)
                     /locus_tag="VD788_03725"
     CDS             complement(2843..>3736)
                     /locus_tag="VD788_03725"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HXV75403.1"
                     /translation="EADGEPEEFTADIVVVACGAINSAALLLRSANERHPNGLANGSD
                     VVGRHYMGHINSVQMAISRCPNPTVFQKTLSLNDFYFGEPDWDYPMGHISFVGKLDRD
                     TLRAGAPALAPGFTLEIMGQHSLDFWLTSEDLPDADNRLTLDREGNIVLRYRPNNEEG
                     HKRLTSRLHWLMQRQTKCGVHGHDCHRGLFARSLFLGQRIPLAGVAHQNGTIRFGHDP
                     RVSALDVNCKAHELDNLYVVDASFFPSSGAVNPALTIIANALRVGDHLLDRMNCHREA
                     DAAASLGAQRAPTAGAGGHRR"
BASE COUNT          606 a         1276 c         1224 g          630 t
ORIGIN      
        1 cgcgggggcc gccccgtaga cggcctggtt gatctccttg tcggtcccgt cgacgccgac
       61 gcagagcacc ttgaccaaca cgccgcgacc gtcgggatag tcgccgacgc cgggcgtcgg
      121 cacgtcgcgc aggtgcagcg aatcgggagt gccggggatc acggccagcg ctttcatcac
      181 accttctcct ctacggcgag cgagcccgcc gtcgcttgcg gcactgcggc cggtgcgcgc
      241 agcagctccg cgaccaggcc ggtccagccg gtctggtggc cggcaccgac gccgcggccg
      301 gtatcgccgt ggaagtactc gtagaacggg acgtggtcgc ggaaatgagg gtcggattgg
      361 agcagcggtt ggtcgcccag taccggtcgc cgaccgcgct cgtcgcgcgt gaagatcgag
      421 gtcagcctgc gggagatctc gtccgccacc tcggccagcg aaacgaaccg tcccgacccg
      481 gtcggacact cgacgcggaa ctggtcgccg taatacgagt ggaaacgacg cagcgaatgg
      541 acgatcaagc cattcaccgg gaaccagatt ggcccgcgcc agttcgagtt cccgccgaac
      601 agcccggagt tcgactcgcc cggctggtag tcgacggtga agcgctgacc ggaccagtcg
      661 agctcgaacg gatgggcgag gtggtgtcgc gacagcgcgc gcacgccgta gtccgacagg
      721 aactccccgg ggtcgagcat ccgcgctagc agccgcttca tcctgtgtcc gcgcaacagc
      781 gacaacaacc gctgctcgcc gcggcccgga acctgccagc gcgagaccag ctcggccaga
      841 tcgggccggt agtcgaggaa ccactcgaga cgccggcgga accccggcac cgcttcaagc
      901 agctccggct ccagcacttc gaccgcgaac aacgggatca gaccgaccat cgagcgcacc
      961 ttcatccgcg agctcctgcc gtccggcgtg tgcagcacgt cgtagtagaa ctcgtcgtcg
     1021 ggatcccata gctcgatgcc gtcgcctccg acgttgttca tcgccgcggc gatgtagagg
     1081 aagtgctcga agaacttggt cgcgacgtcc tcgtagacct cgttctccct ggccagctcg
     1141 agcgcgatgc gcatcaggtt cagactgtac atcgccatcc agctggtgcc gtcggcctga
     1201 tcgatgtgcc cgccggtcgg aagctcggtg ctgcgatcga acacgccgat gttgtccagt
     1261 ccgaggaagc cgccctggaa cacattccgc ccgttgacgt ccttgcggtt gacccaccag
     1321 gtgaagttca gcaagagctt gtggaagacg cgctcgagga agcgcagatc gcccttgccg
     1381 ccgcgctggt tccgatcgat ctcgtagacc cgccaggcac cccaggcgtg gaccggtgga
     1441 ttgacgtcgc cgaacgccca ctcgtaggcc gggagctggc cgttcggatg catgtaccat
     1501 tcgcgtgtga gcagcaccaa ctgctctttg gcgaactccg gatcgaccag cgcgagcgga
     1561 acgcagtgga atgccaggtc ccacgccgca taccacgggt actcccactt gtccggcatc
     1621 gagacgacgt cggcgttgtt caggtgggtc cactccgcgt ttctcccgcg acggcgttcg
     1681 gccggcgggg acggttggcc ggggtcccct tccagccatt gccccacgtc gaagtggtag
     1741 aactgcttgc tccacagcat tccggccagc gcctgccgtt ggatcgagcg tgcctcgtca
     1801 ccctcgatcc ccgactgcag cgcgcggtag aactcgtccg tttcccgacg gcgcgcctcg
     1861 aacacggcgt cggcgtcgcg gaacggcgcg cggcggcgct cgggagacag ccggagctgc
     1921 accgtcgtcg agccgccggc ggcgaccatc cggacgaacc gcgcggcggc cttcgtcccc
     1981 tgccgagcgg gatcgaccgc ctcggcgcga ccgttgatca cgtactcgtg gatcccgtcc
     2041 ttgaagtagc cgggtcgctc gacgccccac agccgtttgg aattggtctc gttttcgcaa
     2101 aacaacagct cgggcgcgcc atccgcgtgg aggaagaact cgccgagccc cgggcagtcg
     2161 acctcgatcg tgctgtcgtc gacggccgcc aatcgcggcc gctcggccga gcgtccccag
     2221 cgccaggtgt tgcggaacca gatctggggc agcagctcga gccgggccgc ggtctccgac
     2281 cggttgtggg ccgtcacgcg cagcagcagc tcgtccggtc ccgccttggc gtattcgatg
     2341 aagacgtcga agtaacgccc gtcgtcgaag atccccgtgt cgaccagctc gaactcgcct
     2401 tcgtgcttgc cgcgacgccg gttctcatcg acgagtcgcg cgtaggggaa ggcactctgc
     2461 gggtacttgt agagcatgcg catgtaggag tgggtcgggt tgttgtcgag gtagtagtag
     2521 agctctttga cgtcctcgcc gtggttcccc tcggagccgg tcagcccgaa cagacgttcc
     2581 ttgaggatcg cgtcctcgcc gttccacaac gcgagcgcga ggcagatccg ctgctgctcg
     2641 tcgcagacgc cgccgatccc gtcctcgccc cagcgatagg cgcggctccg tgcctgatcg
     2701 tgcgagacgt agtcccaggc cgtgccgccg gggctgtagt cctcccgcac cgtgccccat
     2761 tgacgttcgc tgacgtacgg gccccaccgt cgccacggcg cttggcccag atcggcttgt
     2821 cgccgccgtt cgatctcggc gttcatcgtc gatgccctcc cgcccccgcc gtcggtgccc
     2881 gctgcgcacc aaggctcgcc gcggcgtcgg cctcgcggtg gcagttcatc cgatcgagca
     2941 ggtggtcgcc gacgcgcaac gcgttggcga tgatggtcaa cgccgggttg accgcgccgg
     3001 atgacgggaa gaagctggcg tcgacgacgt acaggttgtc caactcgtgc gccttgcagt
     3061 tcacgtcgag cgccgacacc cgcgggtcgt gaccgaaacg gatcgttccg ttctgatgcg
     3121 cgacgccggc cagcggaatg cgctgcccga ggaacaggct gcgtgcgaac aatccgcggt
     3181 ggcagtcgtg tccgtggacc ccgcatttcg tttgacgttg catcaaccag tgcagacgcg
     3241 aggtcagtcg cttgtgcccc tcctcgttgt tcggccggta gcgcagcacg atgtttccct
     3301 cgcggtcgag cgtgagacga ttgtcggcgt ccggcagatc ctccgaggtc agccagaagt
     3361 cgagcgagtg ctgccccatg atctccagcg tgaatcccgg cgcgagcgcc ggcgcgccgg
     3421 cccgcagcgt gtcgcgatcg agcttgccga cgaacgaaat atgacccatc gggtagtccc
     3481 agtccggttc gccgaagtag aagtcgttga gcgacagggt cttctggaac acggtcggat
     3541 tggggcagcg cgagatcgcc atctgcaccg agttgatgtg acccatgtag tgacggccga
     3601 ccacgtccga gccgttggcc agcccgttcg gatgacgctc gttggcggaa cgcagcagca
     3661 acgccgcgga gttgatcgca ccgcacgcca cgacgacgat gtcggcggtg aactcttccg
     3721 gctcaccgtc ggcctc
//
LOCUS       DASPGN010000135         6607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_959277, whole genome shotgun sequence.
ACCESSION   DASPGN010000135 DASPGN010000000
VERSION     DASPGN010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959277"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1101)
                     /locus_tag="VD788_03730"
     CDS             complement(154..1101)
                     /locus_tag="VD788_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV75404.1"
                     /translation="MSRYLITGGAGFIGSNLTEALLRRGHSVRVLDDLSTGRRENLAA
                     AETWRREGGGQFEFVEGDVRDRDTCRAVVRDADFVLHQAAVPSVQRSVLDPLTTNAVN
                     VDGTLNVLEAARAAGVKRLVFASSSSIYGESETLPKVETMAPAPISPYGLQKLAGETY
                     CRLYHRLYGVPTVALRYFNVFGPRQDPTSEYSAVIPRFITAVKSDRPPTIYGDGEQSR
                     DFTFIANVVAANRLACEAGPAALGRSYNVGCGARITLNQLVVEIGRIFGRDVRPTHAP
                     PRVGDIRHSLAAIDAAVEMLGFAPVVGLGEGLERTAEAY"
     gene            complement(1104..2408)
                     /locus_tag="VD788_03735"
     CDS             complement(1104..2408)
                     /locus_tag="VD788_03735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXV75405.1"
                     /translation="MNICVVGTGYVGLVTGACFAEFGNPVTCVDNNASKIELLERGEM
                     PIYEPGLDDLVERNVKAGRLRFSTQLAEAVRDSLVVFIAVGTPQGSDGRADLSFVRDV
                     AKTIASNMNSYKVVVTKSTVPAGTGRMIREVIERHLTERHRFSVASNPEFLREGSAIE
                     DFLRPNRVVLGTEDEQSAAILQDLYRPLYLIETPIVHTDVVTAEMIKYASNAFLATKI
                     SFINEMADLCEAVGADVHGVAKGMGLDRRIGSKFLHPGPGYGGSCFPKDTRAVLEVAR
                     DHQVDLKIVDAVIRVNDARPAAMVEKIRRALGGQITGKTICLLGLTFKPNTDDLRESP
                     AIEILNRVVAAGAGVRAYDPVAAPMLAATPSENVTYCKDEYEAAAGADALVIATEWNQ
                     FRGLDLARLKELLRQPIVVDLRNVYDPDAMRDRGFDYTGVGR"
     gene            complement(2405..3379)
                     /locus_tag="VD788_03740"
     CDS             complement(2405..3379)
                     /locus_tag="VD788_03740"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXV75406.1"
                     /translation="MGGPSILITGGAGFIGSHLSERLLADGRPVVVLDNFDSFYDPAI
                     KRRNVAGLSADGRFRLVEGDIRDSATLDALLAAHPVSAVVHLAARAGVRPSIADPALY
                     TSVNLDGTTALLEACRRHSIDRLVFGSSSSVYGNNDKVPFSEDDRVDRPISPYGATKK
                     AGEVLCYSFHHLTGMKIACLRFFTVFGPRQRPEMAIHKFARRLAAGEAVDQYGDGSSA
                     RDYTYVSDIVEGIVRALDRSTGYHVWNLGGSQTTTLGDLVRKIAAGLGVPPRVRQLPP
                     QPGDVDRTWADVSRARAELDWAPLIPFDAGLERFLDWFRSQPAVEGGR"
     gene            complement(3403..4719)
                     /locus_tag="VD788_03745"
     CDS             complement(3403..4719)
                     /locus_tag="VD788_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HXV75407.1"
                     /translation="MRLEQELKRKIEARQAHIGIVGLGYVGLPLAVEFAKAGFRVTGV
                     DVDREKVRTLRAGRSHIEDVPSTEVAPLVKQRLLRAQDHYRGSGEFDVVVICVPTPLR
                     KTKDPDISYIVSAMDELVREVRKGQLMILESTTYPGTTEEVLRPRIEERGLEIGRDFF
                     LAFSPERVDPGNRQFKTHNTPKVVGGSTPACTDVAVCLYRQAIQTVHPVSSTRVAEMV
                     KLLENTFRAVNIALVNEIALMCERLQLDVWEVIDGAATKPFGFMRFYPGPGIGGHCIP
                     LDPHYLSWKLKTLDYSARFIELASEINGSMPLVVVRRASELLNRKRKSVNGARILVIG
                     VSYKRDTRDLRESPALDIMRLLDQRGAKLSYHDPYNDTVRIGEGKSYRSSKPSAANLS
                     KADLVLIVTDHSSIDYDEIVRHAPLVLDTRNSTARVRHKPSKVHKL"
     gene            complement(4816..5964)
                     /locus_tag="VD788_03750"
     CDS             complement(4816..5964)
                     /locus_tag="VD788_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXV75408.1"
                     /translation="MARPGSRDVAVGALFSLALVILAVTIMAVGEESRLFSEKASYSV
                     VFPSVDGLGVGSPVKMSGVVVGSVQAIRLSADPGTTGIEVEIGIDRTFAPRVREDARA
                     ALRILQLLSGEKFVEIVPGSPGAAPLSAGSVIETQREQALLERAAVTAENLNDITISL
                     KNILSRLEAGEGLLGQMLTDPQFGKEGLEALRSTLENIEEITGDLRAGRGTLGRFLKD
                     EQLARKVDELFSSLDKLTTMINGLDLERGALGALLTEDGAGRRAIEDLAVAAATFREL
                     SERLAAEQGVLGQLLAGGEEPDQFGDDLRRLVRNLAEISGKINSGEGTLGLLVNDRTL
                     YDGMEDVVAGANDSKFARWLLRRYQKRGIEVGEEQDPAAGEESADPDP"
     gene            complement(5957..>6607)
                     /locus_tag="VD788_03755"
     CDS             complement(5957..>6607)
                     /locus_tag="VD788_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV75409.1"
                     /translation="ETVVVLGGSGSGKSVLLRHTIGLHHPDGGEVWVDGERIDHYDEE
                     QLIVPRKKVGMLFQAGALFDSMNVHDNVAYALREHTDWDEGKIGERVREVLGLVELDG
                     VDELMPSDLSGGMRKRVALARAIALSPRGILYDEPTTGLDPITANTINHLIRSLQERL
                     GVTSIVVTHDIHSAFTVGDRIAFLREGKILFDGTVEQARSTTEPTLRNFLEGGGYG"
BASE COUNT         1059 a         2305 c         2169 g         1074 t
ORIGIN      
        1 cgcagccacg attcggcgag cagggtccgc ccaccggtca acgcgagcaa ctgcttgggc
       61 cgtgcggctc ggctgagcgg ccagaaacgg gtgccgctcc cgccggcgag caggacggcg
      121 tggaaccggg cgtcgttggc ccgcagcgcc acctcagtag gcctcggcgg tccgctcgag
      181 cccctcaccg agaccgacga ccggcgcgaa gccgagcatc tcgacggcgg cgtcgatcgc
      241 ggccagcgag tgacggatgt caccgacgcg cggcggggcg tgggtcggcc gcacgtcgcg
      301 gccgaagatc cgcccgatct cgactaccaa ctgattgagc gtgatgcgcg cgccgcagcc
      361 gacgttgtag ctgcgcccga gcgccgcggg tccggcctcg caggccagcc ggttggccgc
      421 gacgacgttg gcgatgaagg tgaagtcgcg gctctgctcg ccgtcgccgt agatcgtcgg
      481 tgggcggtcc gatttgacgg cggtgatgaa ccgcgggatc accgccgagt actcgctggt
      541 cggatcctga cgcggaccga agacattgaa gtaacgcagc gcgacggtcg gcacgccgta
      601 gagccgatgg tacagccggc agtaggtctc gcccgcgagc ttttgcagac cgtagggcga
      661 gatcggtgcc ggcgccatcg tctcgacctt gggcagtgtt tcgctctcgc cgtagatcga
      721 cgagctcgag gcgaacacca agcgcttgac gccggcggcg cgcgcggcct cgagcacgtt
      781 caaggtgccg tcgacgttga ccgcattggt ggtcagcgga tccagcaccg atcgttggac
      841 cgacggcacc gccgcctggt gcagcacgaa gtccgcgtcg cgtacgaccg cgcggcatgt
      901 gtcgcggtcg cgcacgtcgc cctcgacgaa ctcgaactgg ccgccgccct cgcgccgcca
      961 cgtttccgcc gcggcgagat tctcgcgccg gccggtcgac aggtcgtcca acacgcggac
     1021 cgagtgtccg cggcgcagca acgcctcggt caggttggat ccgatgaacc cggcgccgcc
     1081 ggtaatcagg taacgcgaca cgctcacctc cccacgccgg tgtagtcgaa ccctcggtcg
     1141 cgcatcgcgt cgggatcgta gacgttgcgc aggtcgacga cgatcggctg cctgagcagt
     1201 tccttcagcc gcgcgagatc gagaccgcga aactggttcc attcggtggc gatgacgagc
     1261 gcgtcggcac cggcggccgc ctcgtactcg tccttgcagt aggtcacgtt ctcgctcggc
     1321 gtggcggcca gcatcggcgc cgccaccggg tcgtaggcgc gcaccccggc gccggccgcg
     1381 acgacccggt tgaggatctc gatcgccggc gattcgcgca agtcgtcggt gttcggcttg
     1441 aacgtcagcc ccaacagaca gatcgtcttg cccgtgatct ggccgccgag cgcgcgccgg
     1501 atcttctcga ccatcgccgc cggccgcgcg tcgttgactc ggatgacggc atcgacgatc
     1561 ttgaggtcca cctggtggtc ccgtgccacc tcgagcaccg cccgcgtgtc cttcggaaag
     1621 caggagccgc cgtagcccgg tcccgggtgg aggaacttcg agccgatccg ccgatcgaga
     1681 cccatcccct tggccacccc gtgcacgtcg gcgccaaccg cctcgcacag gtcggccatc
     1741 tcgttgatga aggagatctt cgtcgcgagg aaggcgttcg acgcgtactt gatcatctcg
     1801 gcggtgacca cgtcggtgtg cacgatcggc gtttcgatca ggtagagcgg ccggtacaga
     1861 tcctggagga tcgccgccga ctgctcgtcc tccgtgccca acacgacccg gttggggcgc
     1921 aggaaatcct cgatcgccga accctcgcgc aggaactcgg ggttggacgc cacgctgaag
     1981 cggtgtcgtt cggtcaggtg ccgctcgatc acctcacgga tcatccgccc ggtgccggcc
     2041 gggacggtgc tcttcgtcac gacgaccttg tacgaattca tgttcgacgc gatcgtcttc
     2101 gccacgtcgc gcacgaacga caggtcggcg cggccgtcgc ttccctgggg cgtgccgacc
     2161 gcgatgaaga cgacgagcga atcgcgcacc gcttccgcga gctgcgtgct gaatctcagc
     2221 cggccggcct tgacgttgcg ctcgaccagg tcgtcgagac ccggctcgta gatcggcatc
     2281 tcgccccgtt cgagcagctc gatcttcgac gcgttgttgt cgacgcaggt cacgggattg
     2341 ccgaactcgg cgaagcacgc gccggtgacc aacccgacgt acccggtccc caccacgcag
     2401 atgttcatcg tccaccctcc accgccggct gtgacctgaa ccaatcgagg aaacgctcga
     2461 gccccgcgtc gaacgggatc aggggggccc agtcgagctc ggcccgcgcg cgcgagacgt
     2521 cggcccaggt gcggtcgacg tcgcccggct ggggcggcag ctgccggacg cgcggcggca
     2581 cgcccagacc cgcggcgatc ttcctcacca gatcgccgag cgtcgtcgtc tgcgacccgc
     2641 ccagattcca gacgtgatag ccggtgctgc ggtcgagcgc gcggacgatg ccctcgacga
     2701 tgtcgctgac gtacgtgtag tcgcgcgccg aactcccgtc gccgtactgg tcgaccgcct
     2761 ctcccgcggc cagccgtcgt gcgaacttgt ggatcgccat ctccggtcgc tgccgcgggc
     2821 cgaacacggt gaagaaccgc aggcaggcga tcttcatccc ggtcagatgg tggaacgagt
     2881 agcacagcac ctcgccggcc ttcttggtcg cgccgtaggg cgagatcggc cggtcgaccc
     2941 ggtcgtcctc cgaaaacggg accttgtcgt tgtttccgta gaccgacgac gacgagccga
     3001 aaacgagtcg atcgatcgag tgacgccggc aggcctcgag cagtgcggtc gtgccatcga
     3061 ggttgaccga ggtgtacagc gccgggtcgg cgatcgacgg gcgcacgccg gcccgggccg
     3121 cgaggtggac gacggccgag accgggtgcg cggcgagcag cgcgtcgagc gtcgccgaat
     3181 cgcggatgtc gccttcgacc agacggaagc gcccgtcggc gctcaggccg gcgacgttcc
     3241 tgcgcttgat cgccgggtcg tagaacgaat cgaagttgtc gagcacgacg accggccggc
     3301 cgtcggccaa caaccgctcc gacaggtgag agccgatgaa gcccgcgccg ccggtgatca
     3361 gaatggacgg tcctcccatc aaacttcgcc caccagcctc ggctacagct tgtgcacctt
     3421 cgatggcttg tgccggacgc gcgcggtcga attgcgcgtg tcgagcacga gcggcgcgtg
     3481 gcgcacgatc tcgtcgtaat cgatcgacga atggtcggtc acgatcaaca ccaggtcggc
     3541 cttggacagg ttggccgcgc tcggcttgga actgcgatag ctcttgccct cgccgatccg
     3601 caccgtgtcg ttgtacgggt cgtggtagct cagcttggcc ccccgctggt cgagcagtcg
     3661 catgatatcg agcgcgggcg attcacgcag gtcgcgcgtg tctcgtttgt aggagacgcc
     3721 gatgacgagg atccgcgccc cgttgacgct cttgcgcttg cggttgagca gctcgctggc
     3781 acggcggacg acgaccagcg gcatgctgcc gttgatctcg gacgccagct cgatgaacct
     3841 cgccgagtag tcgagcgtct tgagcttcca cgagagatag tgcgggtcga gcgggatgca
     3901 gtgcccgccg atgcccgggc cggggtagaa gcgcatgaag ccgaacggct tggtcgccgc
     3961 gccgtcgatc acctcccaca cgtcgagctg gagccgctcg cacatcagcg cgatctcgtt
     4021 gaccaacgcg atgttcaccg cgcgaaaggt gttctcgagc agcttgacca tctcggccac
     4081 gcgggtcgac gacaccgggt ggacagtctg gatcgcctgc cggtagagac agactgcgac
     4141 gtcggtgcag gccggcgtcg agccgccgac gaccttcggc gtgttgtgcg tcttgaactg
     4201 ccggtttccc ggatcgacgc gctccggcga gaacgccagg aagaagtccc ggccgatctc
     4261 gaggccccgt tcttcgatcc gcggtcgcag cacctcctcg gtcgtgcccg gataggtcgt
     4321 cgactcgagg atcatcaact gccccttgcg cacctctcgc acgagctcgt ccatcgccga
     4381 gacgatgtag gagatgtcgg ggtccttcgt ttttctcagc ggggtcggca cacagatgac
     4441 gacgacgtcg aactcaccgc ttccccgata gtggtcctgg gcgcgcagca ggcgctgttt
     4501 gaccagcggc gcgacctcgg tcgacggcac gtcctcgatg tgcgaacgcc cggcgcgcag
     4561 cgtgcgcacc ttctcccggt cgacgtcgac cccggtcacg cggaagccgg ccttggcgaa
     4621 ctcgaccgcc agcggcagcc cgacgtagcc cagaccgacg atgccgatgt gggcttgtcg
     4681 ggcctcgatc ttgcgcttca gttcctgttc caatcgcacg ccacgctccg caaagtgccg
     4741 gtgcggcacc gagaagccct agaatatatg aggcccggct gcaaacttcc acggcgccgc
     4801 caccggcatc ggcgatcagg ggtcgggatc ggcgctttcc tctccggccg ccggatcctg
     4861 ctcctcgccg acctcgatcc cgcgcttctg gtaacgccgg agcagccagc gcgcgaactt
     4921 gctgtcgttg gcaccggcga ccacgtcttc catcccgtcg tagagcgtcc ggtcgttgac
     4981 caacaaaccg agcgtgccct cgccgctgtt gatcttcccg ctgatctccg ccagattgcg
     5041 caccagccgg cgcaggtcgt cgccgaactg atccggctcc tctccgccag cgagcagctg
     5101 cccgagaacc ccctgctccg cggccagccg ctcgctcagc tcgcggaagg ttgcggcggc
     5161 gaccgccagg tcctcgatcg cgcgacggcc cgcgccgtcc tcggtcaaca gcgccccgag
     5221 cgcgccccgc tcgaggtcga gtccgttgat catcgtcgtc agcttgtcga gcgaggagaa
     5281 cagctcgtcc accttgcgcg cgagctgctc atccttcagg aaacgaccga gcgtgccgcg
     5341 acccgcccgc aggtcgcccg tgatctcctc gatgttctcc agcgtcgagc gcaacgcctc
     5401 gagaccctct ttgccgaact gcgggtcggt gagcatctga cccaacagcc cctcgccggc
     5461 ttccagacgg ctgaggatgt tcttgagcga gatcgtgatg tcgttcaggt tctcggccgt
     5521 gaccgccgcg cgctcgagca gcgcctgctc gcgctgggtc tcgatcaccg agcccgcgga
     5581 aagtggcgcc gcgccgggcg atccgggcac gatctcgacg aacttctcgc cggacagcag
     5641 ttgcaggatg cgcagggcgg cgcgggcgtc ctcgcgcacg cgcggcgcga aggtccggtc
     5701 gatgccgatc tcgacctcga tgccggtggt ccccgggtcg gccgacagcc ggatcgcctg
     5761 caccgaaccg acgaccaccc ccgacatctt gaccggggac ccgacaccga gaccgtcgac
     5821 gctggggaag accacggaat aactcgcctt ctccgagaac agccgcgact cttcgccgac
     5881 ggccatgatc gtgacggcca ggatgacgag cgccagcgag aacagcgcac cgaccgcgac
     5941 gtcgcggctg ccgggcctag ccatagccac ctccctcgag gaaattccgc aacgtcggct
     6001 ccgtcgtcga cctggcctgc tcgaccgtgc cgtcgaacag gatcttaccc tcacgcagga
     6061 aggcgatccg gtcgccgacg gtgaacgccg agtggatgtc gtgggtcacg acgatcgacg
     6121 tcacgccgag gcgctcctgc agactgcgga tcaggtgatt gatcgtgttg gccgtgatcg
     6181 gatcgagccc ggtcgtcggt tcgtcgtaca gaatgccgcg cggggacaac gcgatcgcgc
     6241 gggccaacgc gacccgcttg cgcatgccgc cggacaggtc ggagggcatc agttcgtcga
     6301 cgccatccag ctcgacgaga cccagcacct cgcgtacgcg ctcgccgatc ttgccctcgt
     6361 cccagtcggt gtgctcgcgc aacgcatagg cgacgttgtc gtgcacgttc atcgaatcga
     6421 acagcgcacc ggcctgaaac agcatgccga ccttcttgcg tggaacgatc aactgctcct
     6481 cgtcgtagtg gtcgatccgc tcgccgtcga cccacacctc gccgccatcg ggatggtgca
     6541 ggccgatcgt gtggcgcaac agcacggact tgccggagcc cgagccaccc agcacgacga
     6601 ccgtctc
//
LOCUS       DASPGN010000136         3919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_959310, whole genome shotgun sequence.
ACCESSION   DASPGN010000136 DASPGN010000000
VERSION     DASPGN010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959310"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VD788_03760"
     CDS             complement(<1..571)
                     /locus_tag="VD788_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75410.1"
                     /translation="MVRTASQLLLLGLGAILLSWAGCAYADEGDLGGDREPILGRCEG
                     CEAVFDGLPAALGSRGRIAPEDAVGEPMRIEGTVTDAAGRPAAGVIVYAYHTDAHGIY
                     PRDETARTAAARRHGRLRGWVETDEHGRYQFDTIRPASYPDAVTPAHVHMHVLEVGRC
                     TYVIDSIHFDDDPRLTQTAREELIRGPGGS"
     gene            551..790
                     /locus_tag="VD788_03765"
     CDS             551..790
                     /locus_tag="VD788_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75411.1"
                     /translation="MACGPDHRTQPTLGAIGPPALHEPVTETGFYTWPSTNHLVQDVR
                     LSIATPRRNFGWVLIGDEVTPLAVKRFASREHPDE"
     gene            complement(798..1709)
                     /locus_tag="VD788_03770"
     CDS             complement(798..1709)
                     /locus_tag="VD788_03770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXV75412.1"
                     /translation="MIYDYVTLDVFTDRCFGGNPLAVLPAADGLTTKQMQAIAREFNY
                     SETTFVLPPADDNNTRKVRIFTPLAELPFAGHPNVGTAVALARLELLGDAVAGQRLLF
                     EEGAGTVSVFLRGNHVAIDAAEFIAPVINRLEDELTHEEGAKALGLKTAQVVTEHHPP
                     RIVSSGVRFLFIELRDLDALAASEAAQGPVTRISVARGLDGVFGYVRTGDNGVDFRAR
                     MFAPVHGVMEDPATGSANAGLANLLGSMHEERDATLTWTVAQGVEMGRPSRLLLTAER
                     RGGKIVEVRVAGGAVIVCRGQIDVPER"
     gene            1815..2189
                     /locus_tag="VD788_03775"
     CDS             1815..2189
                     /locus_tag="VD788_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75413.1"
                     /translation="MRLALYAAALLTLLVGLAHSYLGERYILTRLFRRDDLPRLFGRA
                     EFTRRTLRFAWHLTTISWWGFAAILVVLADPPATSSALGAILGCTFLAHAAVTIVASR
                     GRHLAWPVFLAIGVIAIAATRG"
     gene            2225..3298
                     /locus_tag="VD788_03780"
     CDS             2225..3298
                     /locus_tag="VD788_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXV75414.1"
                     /translation="MPHPSEPGPRELLLRTGFGYVASITLNVAIELRIADLLGKGPRP
                     IEELARQSSADEDALYRALRLLASLGIFVEDPRRTFGNTPASELLRARGDESLHDTIR
                     WLSDPFHLRIYAELLHSVRTGEPCFDKLFGKPIFEYLPHDPRESEIFNSAMTNFSASL
                     IPAVLDVYDFGGIGTLVDVGGGHGEILCSILEKYPRMNGVLCDLAHVLDGAAEVIGRH
                     GVGDRLRREPIDFFEGVPAGGDAYILKHIIHDWDEARARTILENIATAIGDRPGRVLL
                     IEMVLAPGNAPHLGKFLDIEMLALPGGRERSEEEFRSLFASAGFDLARVIETESPVCV
                     IEAVPSAARRARRPTAASPTADR"
     gene            3574..>3919
                     /locus_tag="VD788_03785"
     CDS             3574..>3919
                     /locus_tag="VD788_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75415.1"
                     /translation="MLGGGPEQAPLHSRGAGRGQPDVPEVGIAEFRHLRFLGDQGVGR
                     LAGVPGIARPAETVERLAEPEVVTEKRMHEDRASLEPTRPDRPRRDRHGRDEQHDRRE
                     PAALGVERETAGD"
BASE COUNT          577 a         1391 c         1358 g          593 t
ORIGIN      
        1 cgcttccgcc ggggccgcgg atcagctctt cccgcgccgt ctgcgtcagg cgcggatcgt
       61 cgtcgaagtg gatcgagtcg atgacgtagg tgcagcgacc gacttcgagc acgtgcatgt
      121 ggacgtgagc cggcgtcacc gcgtcgggat agctcgccgg acggatcgtg tcgaattggt
      181 accgtccgtg ctcgtccgtc tcgacccagc cgcggagccg gccgtggcgt cgcgcggccg
      241 ccgtgcgcgc cgtctcgtcg cgcgggtaga tgccgtgcgc atcggtgtgg taggcgtaga
      301 cgatcacgcc ggccgccggt ctcccggcgg cgtcggtgac cgttccctcg atgcgcatcg
      361 gctcgccgac cgcgtcctcg ggagcgatgc ggccgcggga gccgagcgcc gccggcagac
      421 cgtcgaagac ggcctcgcac ccctcgcacc gtcccaggat cggctcgcgg tcgccaccga
      481 ggtctccctc gtccgcgtac gcacagccgg cccacgacaa caggatcgcc ccgagcccga
      541 gcagcaggag ttggcttgcg gtcctgacca tcgcacgcag cctactctcg gggcgatcgg
      601 cccgccagcg ctgcacgagc ccgtcaccga gaccgggttc tacacttggc cgagcacgaa
      661 tcacctcgtg caggacgtgc ggctgtcgat cgcgacgccg cggcgcaact tcggctgggt
      721 gctgatcggt gacgaggtca cgccgctggc ggtcaagcgc ttcgcgagcc gggagcatcc
      781 cgacgagtga cgcgcgacta ccgctccggc acgtcgatct gtccgcggca gacgatgacc
      841 gcgccgccgg cgacccgtac ctcgacgatc ttgccgccgc gtcgctcggc cgtcagcagc
      901 agccggctgg gccgacccat ctccacgccc tgggccaccg tccaggtcag cgtcgcgtcg
      961 cgctcctcgt gcatcgagcc gagcaggttg gcgagcccgg cattcgcgct gcccgtcgcc
     1021 gggtcttcca tcacgccgtg gaccggcgcg aacatccgcg cgcggaagtc gacgccgttg
     1081 tcgccggtgc ggacgtagcc gaagacgccg tcgagaccgc gggcgacgga gatccgcgtc
     1141 accgggccct gggccgcctc ggaggcggcg agcgcgtcga gatctcgcag ctcgatgaac
     1201 aggaaacgca cgccggagga gacgatccgc ggcgggtggt gctcggtgac gacctgcgcc
     1261 gtcttcagtc ccaacgcctt cgccccttcc tcgtgggtca gctcgtcttc gagccggttg
     1321 atcaccgggg cgatgaattc ggccgcgtcg atcgcgacgt ggttgcctcg caggaagacc
     1381 gagacggttc cggcgccctc ctcgaacagc agccgctggc ccgcgaccgc gtcgccgagc
     1441 aactcgagcc gggccagcgc gaccgcggtt cccacgttgg gatggccggc gaacgggagc
     1501 tcggccagcg gggtgaagat ccgcaccttg cgcgtgttgt tgtcgtctgc cggcggcagc
     1561 acgaacgtcg tttcggagta gttgaactcg cgggcgatcg cctgcatctg cttcgtcgtc
     1621 aagccgtcgg ccgccgggag cacggccagc ggattgccgc cgaagcaacg gtcggtgaac
     1681 acgtcgagcg tcacgtagtc gtagatcacg gagttctccc ttcgctggcg gacgtcgaca
     1741 cgctatcaat cgcggccgat ccgttccagg ggagcggccg gatccggtcg tggtatcctc
     1801 cggccggcac gcccatgcga ctcgcgctct acgccgccgc cctgctcacg ctgctcgtcg
     1861 ggctcgccca ctcctatttg ggcgagcgct acatcttgac ccgcctgttt cgccgggacg
     1921 acctgccgag gctgttcggc agggccgagt tcacccggcg cacgcttcgt ttcgcctggc
     1981 acctcacgac gatctcttgg tggggcttcg ccgcgatact ggtcgtgctg gccgatccgc
     2041 cggcgacgtc gagcgcgctc ggcgccatcc tcggctgcac gttcttggcc cacgcggcgg
     2101 tcacgatcgt cgcctcccgc ggcaggcacc tggcctggcc ggtcttcctg gcgatcggcg
     2161 tcatcgcgat cgccgccacg cgcgggtgac tcgaaccgag ccgccgcccg caggaggttg
     2221 aaagatgccc catccgagcg aacccggtcc ccgagagctg ctgctgcgca ccggtttcgg
     2281 ctacgtcgcg tcgatcacgc tcaacgtcgc gatcgagcta cggatcgccg acctgctggg
     2341 caagggcccg cgaccgatcg aggagttggc ccggcaaagc tcggccgacg aggacgcgct
     2401 ctaccgagcg ttgcgcttgc tggccagcct cggcatcttc gtcgaagatc cgcggcgtac
     2461 gttcggcaac acgccggcgt ccgagctgct gcgcgcccgc ggcgacgagt cgttgcacga
     2521 cacgatccgc tggttgagcg acccgttcca tctgcggatc tacgccgagc tgctccactc
     2581 cgtccgcacc ggcgaaccgt gcttcgacaa gttgttcggc aaaccgatct tcgagtacct
     2641 gccgcacgac ccgcgcgaat ccgagatctt caacagcgcg atgacgaact tcagcgcctc
     2701 gctgatcccg gccgtgctcg acgtctacga cttcggcggc atcgggacgt tggtcgacgt
     2761 cggcggaggg cacggcgaga tcctctgctc gatcctcgag aaatacccgc ggatgaacgg
     2821 cgtgctgtgc gatctggccc acgtcctcga cggtgccgcg gaggtgatcg ggcggcacgg
     2881 cgtgggtgac cgcctgcggc gcgagccgat cgacttcttc gagggcgtgc cggccggagg
     2941 cgacgcgtac atcctcaagc acatcattca cgactgggac gaggcccgcg cgcggacgat
     3001 cctcgagaac atcgcgaccg cgatcggtga ccggcccgga agggtgctcc tgatcgagat
     3061 ggtgctggcg ccgggcaacg cgccgcatct gggcaagttc ctcgacatcg agatgctcgc
     3121 ccttcccggc ggcagggaac ggagcgagga ggagttccgg tcgttgttcg cctcggccgg
     3181 attcgacctg gcgcgcgtga tcgagaccga atcgccggta tgcgtgatcg aggcggtgcc
     3241 gagcgcagcg cgtcgcgcac gccgcccgac cgcggccagc ccgaccgccg acaggtaggc
     3301 cagcgccggg aagacctgga cgctcatccg cgccatcggc caggtcaggg cgtaggccag
     3361 gttgtgcagc gcgatcgtca tcacgagcgg cacgacgagc gggtcgggcg gtgcgcgcca
     3421 gaccgggctc acgaccccga tcacggccag cagcaacagg cacgccgcgg cgatcgcgta
     3481 ctgggtcgag cggcgcggct cgtcggcgcc gatccagaag cgcgcgagac cctcgaccga
     3541 gcggcgcgca tacgatgccg gccggtcgac gacgtgctcg gcggcggccc ggagcaggcg
     3601 ccgctccatt cgcgcggcgc cggccggggt cagccggacg taccagaagt tggcatcgcg
     3661 gaattccggc atctgcgctt tctcggcgat cagggcgtag gccgtctcgc gggtgtgccc
     3721 ggcatcgcgc gccccgccga gacggtagag cgcctcgccg aaccagaagt tgtaaccgag
     3781 aagcgaatgc acgaggaccg ggcgtcgctc gagccgactc gaccggatcg accacgccgc
     3841 gaccggcacg gccgcgacga gcagcacgat cgccgcgagc ctgcggcgct tggggtcgag
     3901 cgcgagaccg caggtgacg
//
LOCUS       DASPGN010000137         4062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_258084, whole genome shotgun sequence.
ACCESSION   DASPGN010000137 DASPGN010000000
VERSION     DASPGN010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258084"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1904
                     /locus_tag="VD788_03790"
     CDS             <1..1904
                     /locus_tag="VD788_03790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV75416.1"
                     /translation="RDVEVGVRLLAERPTLGRLVDGGDHDARDRFLARFAATGGLDGC
                     GVLGVDGPPGAGELWWGRVTTATTEWQLIEADDGDLPWLAAARPMPGPRGVWVACGRR
                     IDEAVTTQVAGQVGLPVRILSASEARASAGGRLAAMLGAAAGDGRTAAGRDDAIDAFV
                     DVRPLDDPSGAVHGVIETRLAAGAVDASVRRLERAIWIALAVAVPVMVVAAFVLARRL
                     TAPLAELTRASARIGAGEMTVPVPRWARGEVAQLATTMEDMRTALLSLTSELRRRHAE
                     LASILNGVVEGVWAVDRNRRITWINRQAAERLGIRAEDALGKFCGDVLEPRLPGGARP
                     CDTRCPIVDARFRGSARAVEHLAQGDEARRTVVITSAAPADADGDDEGEVRQFQVMRD
                     ETDAEAGRRLRDALLANVSHEFRTPLSAQIASLEMLKERIEEDADPDTLALVRATERG
                     TLRLQRLVDNLLESLRLDSGVLSIRTRPVRLEQVIDQAVEQITPLVEQKRQSLTLSVP
                     ASYPELPGDGPRLVQVLVNLLANANKFAPAGTAIRLGGEVSDTRLAIWVQDEGPGFPE
                     EYDAVEFAPFARASTEEPAEPGIGLGLYIAHSIVTRHGGRLVASNTRPGARVSIVFPR
                     EAADEDPRR"
     gene            1885..2568
                     /locus_tag="VD788_03795"
     CDS             1885..2568
                     /locus_tag="VD788_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV75417.1"
                     /translation="MKILAVDDDLELLGLVAFALRQAGYLVIEAADGTAGLAAFVEER
                     PDLVILDVNLPGMNGFQLLRELRTRSRTTPVMMLTVRSDEQDQVGALDLGADDYLTKP
                     FSPRTLLARVRALLRRAGNERAPVARVGILSVDAERRCVTVGAAEAVHLTALEFRLLQ
                     ILVANAGSVVSADRLSTHVWGRRKSGDRRALKQLIHRLRSKIELDPADPRLLVTEPGE
                     GYRMVAEPE"
     gene            2626..2961
                     /locus_tag="VD788_03800"
     CDS             2626..2961
                     /locus_tag="VD788_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75418.1"
                     /translation="MAHRFVSSVVLIALVVVFWGSVGGFAAGREDAGEPCLGCADCDS
                     GPCDADGVNPLTSHHHCCTTCCVSHAPLALVVPRSAQSPVTPTTMVTPKVAPTADRSP
                     ETPHLPPRV"
     gene            3044..>4062
                     /locus_tag="VD788_03805"
     CDS             3044..>4062
                     /locus_tag="VD788_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV75419.1"
                     /translation="MRRSSLVVPGIVALLSAPAGGEPGTPAYTIEEIRAVARSVHPTL
                     ESVEAGVEAASESVHQARAYPNPALAAAFGRGRPSSGGDARNESSFGLLQPIELPGVR
                     KWRIRLAELGVRAAEVERAVAESIVDSTVARLAHTVLGGEQSVEIARDSLDVAARLHE
                     LLARRVELGEAPPLDAVKARSEWYARQQELLVAESELELARSTLDLFCGRRLGTSFVV
                     TDVLDAGDPTALPDDLVERLQAGNPVLLGAELAIEQAGARIEIERKAPLPRIDVVAGH
                     DTELDRSATHVGVGLTVPLWNRNRGAVAAATASRHRLTSDLAGLRNELEADLARAATA
                     YRRASS"
BASE COUNT          551 a         1404 c         1500 g          607 t
ORIGIN      
        1 ggcgcgacgt cgaggtcgga gtgcggctcc tggccgagcg cccgaccctg ggacgcttgg
       61 tcgacggcgg cgatcacgac gcgcgagatc gtttcctcgc gcggttcgcc gcgaccggtg
      121 ggctcgatgg ctgcggcgtg ctcggcgtgg acgggccacc cggcgccggc gaattgtggt
      181 ggggccgggt gacgacggcg acgacggaat ggcagctgat cgaggccgac gacggcgatc
      241 tgccgtggtt ggcggcggcc cggccgatgc cgggccctcg cggggtctgg gtcgcgtgcg
      301 gacgacggat cgacgaagcg gtcaccacgc aggtcgcggg tcaggtcgga ctgccggtgc
      361 ggatcctgtc ggcgagcgag gcgcgcgcga gcgcgggcgg ccggctggcg gcgatgctcg
      421 gtgcggcggc cggcgacgga cggacggcag ccggccgaga cgacgcgatc gacgccttcg
      481 tcgacgtccg accgttggac gatccgtccg gcgcggtcca cggcgtgatc gaaacgcggc
      541 tcgccgcggg agcggtcgac gcgtcggttc gccggctgga acgagcgatc tggatcgcac
      601 tcgccgtcgc ggttcccgtg atggtggtgg cggcgttcgt cctcgcccgc cggttgacgg
      661 cgccgctcgc ggagctgacg cgggcatcgg cccggatcgg cgccggcgag atgaccgtgc
      721 cggtgccccg ctgggctcgc ggcgaggtgg cgcaactcgc cacgacgatg gaggacatgc
      781 gcacggcgct gctttcgctg acctccgagc tgcgccgccg ccacgccgaa ttggcgtcga
      841 tcctcaacgg cgtcgtcgag ggcgtttggg cggtcgaccg caaccgccgg atcacctgga
      901 tcaaccgaca agccgccgag cgactgggga ttcgcgcgga ggatgcgctc ggcaagttct
      961 gcggggacgt gctcgagccc cgcctccccg gcggcgcgcg gccgtgcgat acgcgctgtc
     1021 cgatcgtcga tgcccggttc cggggcagcg cgcgcgccgt cgagcatctg gcgcagggag
     1081 acgaggcgcg acgaacggtc gtgatcacga gcgcagcgcc ggccgatgcg gacggcgacg
     1141 acgaggggga ggtgcggcag ttccaggtga tgcgcgacga gaccgacgcc gaggccgggc
     1201 gccgcctgcg cgacgcgttg ctggcgaacg tctcgcacga gttccgcaca ccgttgtcgg
     1261 cccagatcgc ctcactcgag atgttgaagg agcgaatcga ggaggacgcc gatccggaca
     1321 ccctggcgct cgtccgcgcc accgagcggg gtaccctccg actccagcgt ctggtcgaca
     1381 atctgctcga gagtcttcgt ctcgactcgg gcgtgctgtc gatacggacc cggcccgtgc
     1441 gcctcgagca ggtgatcgat caagcggtcg agcagatcac gccgctcgtg gagcagaagc
     1501 ggcaatcgtt gacgttgtcg gtgccggcgt cgtaccccga gctgccgggg gacgggcccc
     1561 ggctcgtaca ggtgctggtg aacctgctgg cgaacgcgaa caagttcgcg ccggcgggga
     1621 cggcgatcag gctcgggggc gaggtgagcg acacgcggct cgcgatctgg gtccaggacg
     1681 agggccccgg ttttcccgag gagtacgatg ccgtcgagtt cgcgccgttc gcccgggcct
     1741 cgacggagga gccggccgag cccggcatcg gtctgggact ctatatcgcg cactcgatcg
     1801 tgacccgcca cggagggcgg ctcgtcgcga gcaacacccg acccggggcg cgggtgagca
     1861 tcgtctttcc gcgggaggcc gccgatgaag atcctcgccg ttgacgacga tctcgagctg
     1921 ttggggctgg tggcgttcgc gctgaggcag gccggctacc tggtcatcga ggccgccgac
     1981 ggcaccgccg gtctggcggc gttcgtcgaa gagcgcccgg atctcgttat cctcgacgtc
     2041 aacctgccgg ggatgaacgg gttccaactg ctgcgcgaat tgcggacccg gtcccgcacg
     2101 acgccggtga tgatgctgac ggttcgctcg gacgagcagg accaggtcgg cgcgctcgat
     2161 ctcggggccg acgattacct gaccaagccg ttcagcccgc gcacgctgct tgcccgggta
     2221 cgggcgctgc tccggcgagc cgggaacgag cgggcaccgg tcgctcgcgt cggcatcctg
     2281 tcggtggatg ccgaacggcg ttgcgtcacg gtcggcgcgg ccgaggccgt ccacttgacc
     2341 gccctcgagt tccgcctgct gcagatcctg gtggcgaacg ccgggtcggt cgtttcggcc
     2401 gaccggctga gcacccacgt ctgggggcgt cgcaagtccg gggaccgccg ggcgctcaaa
     2461 caactgatcc accgcctgcg ctcgaagatc gagctcgacc cggccgatcc gcgtttgctc
     2521 gtgaccgaac ccggtgaggg ctaccggatg gtggccgagc cggaatgacc agcgcgcgac
     2581 ccggcggagt tgacgcgtct cggttccgcg cctatcgttg gcgcgatggc tcatcgcttc
     2641 gtgtcgagcg tggtgctgat cgcgctggtc gtggtcttct ggggctcggt cggcggtttc
     2701 gccgccggcc gggaagatgc cggcgagccg tgcctcggtt gcgccgactg tgattccggt
     2761 ccgtgcgacg ccgacggagt caacccgctg acgtcgcacc atcattgctg caccacctgc
     2821 tgcgtctcgc acgcaccgct cgcgctggtg gtcccgcgct cggcgcagtc gcccgtgacc
     2881 ccgacgacga tggtgacccc caaggtcgcg ccgaccgccg atcggtcgcc ggaaacgcct
     2941 cacctccctc ctcgcgtctg atctcctcgt tgacgctcgc gggtcgagtc gatcgacctc
     3001 gcccgcccat gttcgcgacc ggcgaacgag gaaggattcc ggaatgcgtc gttcatctct
     3061 ggtcgtaccc ggcatcgtcg cgctgctgtc ggcgcccgcc ggcggcgagc cgggtacgcc
     3121 cgcatacacg atcgaggaga tccgagccgt cgcccgatcc gtccacccga cactcgagtc
     3181 ggtcgaggcc ggagtcgagg cggcgagcga atcggtgcat caggcacggg cctatccgaa
     3241 tccggcgctc gccgctgcct tcggccgcgg ccggccgagc agcggcggcg acgcgcgcaa
     3301 cgagagttcg ttcgggctgc tgcagccgat cgagctcccc ggcgtgcgga agtggcggat
     3361 tcgcctggcc gagctcggcg tacgcgctgc cgaggtcgag cgtgccgtcg ccgagtcgat
     3421 cgtcgactcg accgtcgccc gcctggcgca taccgtcctc ggcggagagc agagcgtcga
     3481 gatcgcgcgc gacagcctgg acgtcgcggc gcgccttcac gagttgttgg ctcgccgggt
     3541 cgagctcggc gaggccccgc cgctcgacgc ggtcaaggct cgcagcgagt ggtacgcacg
     3601 gcaacaggag ctgctcgtcg ccgagagcga gctcgagctg gcgcgttcca cgctcgacct
     3661 gttctgcggc cgtcgcctcg ggacgagctt cgtcgtcacc gacgtgctcg acgcgggaga
     3721 cccgaccgcg cttcccgacg acctcgtcga gcgcctgcag gcgggcaatc ccgtcctgct
     3781 cggtgccgag ctggccatcg agcaggccgg agcccggatc gagatcgaac ggaaggcgcc
     3841 gcttccgcgc atcgacgtcg tcgccggcca cgacacggag ctcgatcgca gcgcgaccca
     3901 cgtcggcgtc ggtctgacgg tcccgctgtg gaaccgcaac cgcggcgcgg tcgccgcggc
     3961 caccgcgagc cggcaccggt tgacgagcga cctcgccggt cttcgcaacg agctcgaagc
     4021 cgacctcgcc cgggccgcga ccgcctatcg gcgcgcctcg tc
//
LOCUS       DASPGN010000138         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_258132, whole genome shotgun sequence.
ACCESSION   DASPGN010000138 DASPGN010000000
VERSION     DASPGN010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258132"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="VD788_03810"
     CDS             <1..1630
                     /locus_tag="VD788_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HXV75420.1"
                     /translation="GFSNPAVVTIMMMFILSEGLVQSGVVTRIGYALMRLAGRATERA
                     HWPLLLVTASISAFINNTAAVSLFIPVALSVAKHFRLSPSKVLLPLSYSAIFGGTCTL
                     IGTSTNILVSTLAVDHGLAPFTVFEFLRVGVFFFVGGMAFNFFLAPRLLPDRTDQRDL
                     TGKYRMGSYLTELRVARGSDLIGRTLKQERFGERYHLSVLEIVRGGAKIVDGIDATPL
                     GENDVLIVSGKIDDILSVKERRGLLMLTDVKLGDAELADRSNVLAEIQLSPRSDLLGA
                     TLKEIDFRRRFGCFVLALNRTGDVIREKLTSVPLHAWDTLLVFGPRASIEAQGSGRDF
                     VLLQELPVRLRLMRRWWIGAATVPAVVALAAFDVTSIMVSAILGVALLLATRTLRIHQ
                     AYQSIDWTVIFLLVTVLPVGVAIESTGLAAAIGEGIVRVGQPLGAWVVLSLVILATSL
                     LTEAITNNSAAVLMVPICVSAAHELGVDPKPFVMGVTFAASMSFATPTGYQTNTMVYG
                     PGSYRFTDYLRVGVPLNLLFWALASVLIPVVWPF"
     gene            complement(1641..>2752)
                     /locus_tag="VD788_03815"
     CDS             complement(1641..>2752)
                     /locus_tag="VD788_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75421.1"
                     /translation="PFYWGFFPYLVATPVGVWALAAAIRASSRPTPPRVAGVAALGVL
                     LLVSHVLVFGVALAMSVAYTLAESPGRRALRALVGWLPAGLLLVPWLVLDFERPSRGG
                     WDWSLEPSRLLQLASGVAGDARDGPTGWAVLGAVAALGLVAGIRLRPRRGNLALAACG
                     LALAALSPAVAFGISHLSWRLAFWFWLGCATLVAPLPSSPRRQIARLATGLALTLSAG
                     WIGGQIRAFDRDARTFDPILAAMAPGKYLHPLLFDFQSAVGPSARHLHGYLHFGGWYY
                     VEKRGLYAGMFRFAPQQLPIVRTAPLFGDRFTEDSSYWSAASFEREPAGAYDYYLLRQ
                     LSEKRAEAFLDSHPALEEIRRSGAWYLLRPAAAAR"
BASE COUNT          439 a          949 c          948 g          416 t
ORIGIN      
        1 gggattctcg aacccggccg tcgtgacgat catgatgatg ttcatcctga gcgagggact
       61 ggttcagagc ggcgtggtga cgcggatcgg ctacgcgctg atgcgcctgg cgggcagggc
      121 gaccgaacga gcgcattggc cgctgctgct cgtcaccgcc agcatttcgg cgttcatcaa
      181 caacaccgcg gccgtctcgc tgttcatccc cgtcgcgctc agcgtggcca agcacttccg
      241 cctcagtccg tcgaaggtgc tgctcccgct ctcctactcg gcaatcttcg gcggcacgtg
      301 cacgctgatc ggtacgtcga ccaacatcct ggtcagcacg ctcgccgtcg atcacggact
      361 cgcgccgttc accgtgttcg agttcctgcg tgtcggcgtc ttcttcttcg tcggcgggat
      421 ggcgttcaac ttcttcctcg cgccgcgtct gctccccgat cggaccgatc aacgtgacct
      481 gaccgggaag taccggatgg gctcgtacct gaccgagctg cgcgtggcgc gcggatcgga
      541 tctgatcggc cggacgctaa agcaggagag attcggcgag cgctaccacc tgtcggtgct
      601 cgagatcgtc cgcggcggcg ccaagatcgt cgacggcatc gacgccaccc cgctcggcga
      661 gaacgacgtg ctgatcgtgt cgggcaagat cgacgacatc ctgtcggtca aggagcgacg
      721 cgggctgctg atgctgaccg acgtcaagct gggcgacgcc gagctggccg accggtcgaa
      781 cgtcctcgcc gagatccagc tgtcgccgag atccgacctg ctcggcgcga cgctgaagga
      841 gatcgacttc cgtcggcgct tcggctgttt cgtcctggcg ttgaaccgaa ccggcgacgt
      901 gatccgcgag aagctgacgt cggtcccgct gcacgcgtgg gacacgctgc tcgtgttcgg
      961 cccgcgagcg agcatcgagg cgcagggctc gggtcgtgac ttcgtgctgc tgcaggagct
     1021 gccggtccgc ctgcggctga tgcggcgctg gtggatcggg gcggcgaccg tgccggcggt
     1081 cgtcgcactg gccgcgttcg acgtcacgtc gatcatggtc agcgcgatcc tcggtgtcgc
     1141 gctgctgttg gccacgcgga cgctgcgcat ccaccaggcc taccagtcga tcgactggac
     1201 cgtcatcttc ctgctggtga ccgtgctccc ggtcggcgtg gcgatcgaga gtaccggtct
     1261 cgctgccgcg atcggcgaag ggatcgtccg cgtcggccaa ccgctcggcg cgtgggtcgt
     1321 cctgtcgctc gtgatcctcg cgacctcgct cttgaccgag gcgatcacca acaattcggc
     1381 cgcggtgctg atggtcccga tctgcgtgtc ggcggcccac gagctgggag tcgatccgaa
     1441 gccgttcgtg atgggtgtga ccttcgcggc gtcgatgagc ttcgccacgc cgaccggcta
     1501 ccagacgaac acgatggtct acggtcccgg ctcctaccga ttcaccgact atctgcgcgt
     1561 cggcgtaccg ctcaatctgc tgttctgggc gctggcgagc gtgctgatcc cggtcgtctg
     1621 gccgttctga tcgccgcggt tcagcgagcc gccgcggcgg gtcgcaacag gtaccacgcc
     1681 cccgagcgtc ggatctcctc gagtgccgga tgggagtcga gaaacgcctc ggcgcgcttc
     1741 tcgctcaact gtcggagcag gtagtaatcg tacgcgccgg caggctcgcg ttcgaacgag
     1801 gccgcggacc agtacgacga atcctccgta aagcggtcac cgaacagcgg cgcggtcctg
     1861 acgatcggca gctgctgcgg agcgaagcgg aacatgccgg cgtagagccc acgcttctcc
     1921 acgtagtacc agccaccgaa gtgcagatag ccgtgaaggt gacgcgccga cgggccgacg
     1981 gcgctctgga agtcgaacag cagcgggtgc aggtacttgc cgggggccat cgcggcgaga
     2041 atcggatcga acgtccgcgc gtcgcggtcg aacgcccgga tctggccgcc gatccagccg
     2101 gccgacagcg tcagcgccag cccggtcgcc aggcgcgcga tctgccgtcg cggcgacgac
     2161 ggcaacgggg cgacgagcgt cgcacatccg agccagaacc agaacgcgag acgccagctc
     2221 aggtgggaga tgccgaacgc gacggcgggg gacagagcgg ccagcgccag cccgcaggcg
     2281 gccagcgcca gattcccccg gcgcgggcgc aggcggatcc cggcgacgag cccgagcgcc
     2341 gcgacggcgc cgagcaccgc ccaaccggtc ggcccgtcgc gggcgtcacc cgcgaccccg
     2401 gaggcgagct ggagcagccg gctcggttcg agcgaccaat cccacccacc gcgggacggc
     2461 cgctcgaaat cgaggaccag ccatggcacg agcagcaggc cggccggcag ccaccccacg
     2521 agcgcgcgca acgcccgtcg ccccggggac tccgccaggg tgtaggcgac gctcatcgcg
     2581 agcgcgacac cgaagaccag gacgtgcgac accagcagca ggacgccgag cgccgccacc
     2641 ccggccacac gcggcggcgt gggccgcgag ctcgcgcgga tcgcggcggc gagcgcccag
     2701 accccgacgg gggtcgcgac caggtagggg aagaaccccc agtagaacgg gt
//
LOCUS       DASPGN010000139         6253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_374193, whole genome shotgun sequence.
ACCESSION   DASPGN010000139 DASPGN010000000
VERSION     DASPGN010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374193"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1672
                     /locus_tag="VD788_03820"
     CDS             <1..1672
                     /locus_tag="VD788_03820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXV75422.1"
                     /translation="TELPLDVGGFVVSPDGSRLALSLDVFVDCDTPECTSKRLEGRRQ
                     EKASGRIYERLFVRHWDGWKDGRRSHLFVVSLDDGTAVDAMRGMDADCPSKPFGGPEE
                     YAWTPDGSEIVFTARDVGREEAWSTDFDLFRTPADGSSAPTKITSANRAWDTQPVFSP
                     DGATLAYLAMSRPGYESDRFRIVLMDWPAGEPRVLTEGWDRSPGSIGWSPDRKTIYAT
                     AANLGQSSLFAIDVDDGAVRTLVETGTVRAILPAAERIVFARDELRRPVELFSIRVDG
                     GDEQRLTHINDDYVAAARMGEPEQFDFAGWNGERVHGYVVKPVDFDPKRRYPVAFLIH
                     GGPQGSFGNDFHYRWNPQAYAGAGYAVVMIDFHGSVGYGQRFTDSINGDWGGKPLDDL
                     QQGLAAALARYPFMDADRICALGASYGGYMINWIAGNWPGRFRCLVNHDGNLDERMAY
                     YDTEELWFPEWERGGPPWEVPQAYAEHNPIDHVAEWKTPMLVIHGALDYRVVDTQGLS
                     TFTALQRRGIPSKLLYFPDENHWVLKPHNSILWHETVLDWIDRWTEPR"
     gene            1683..1904
                     /locus_tag="VD788_03825"
     CDS             1683..1904
                     /locus_tag="VD788_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75423.1"
                     /translation="MTRRRGQGRDLLLAIFLVSALAALIWPIYPLVGSSIEPRVLGLP
                     FSLAWVVIWLVLSFLAMWIYHVSGSDERG"
     gene            1906..3417
                     /locus_tag="VD788_03830"
     CDS             1906..3417
                     /locus_tag="VD788_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HXV75424.1"
                     /translation="MPSWVIVFAVAVVYLALSLLVGMHPGRRATKTASGFVAGDRSLG
                     LFVMYFITGATIFSAFAFLGAPGWVYSRGAAAFYILAYGCLAFLPFYFLGPRAARLGR
                     RFGFVTQAEMVAERYGSRALAGLMALVSVLGFIPYLALQMKGAGYVLASVTRGHVPEW
                     LGAAIVYGVVLIYVMRSGVLGVGWTNTFQGILMMGLAWGLGLYLPYELYGGVGPMFDA
                     IAETRPELLRAPGLAASGESWSWGEYGSAVVISIVGFSVWPHLFMKAFTARDDRTIRR
                     TVVLYPTFQLFLVPLFLIGFSGVLFNPAPSSPDQILPHMLMQMELPAVLVGLFCAGAL
                     AASMSSGDAMAHAAASIAVRDGVVTTLGVSLDERQQRDWIRVGVVLSMIAAYVLTVSY
                     DKDLVALLLYAYGPIGQFAPVVFATLYSRRATGAGVFAGLLAGSLVTIVLDVRPDWRP
                     WPIHAGVYGLVANLVTLAIVSAATPQRRDAGREDRYLDVAAGRSTDAAPAALR"
     gene            3432..4655
                     /locus_tag="VD788_03835"
     CDS             3432..4655
                     /locus_tag="VD788_03835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV75425.1"
                     /translation="MGARFNLRTLDRGQRRVLGTTCAAHALIHVYELSLPALLLLIQD
                     EFDAGDLEMGGIVMLSALLFGLGALPAGYLVDRLGSKPLLVGCLWGGSLSLGAMAFSP
                     SAPSFAVAAALLGLALSIYHPAGTALITHAIPLSGKVFALHGMAGNLGVAGAGVVAGT
                     LGAAFGWRWAVALLAGCGLLLGGRALLLPAPALQEIRDRSGRGSWHAFAGLLVATMFM
                     GMVYRGLTTFLPKFFAVSFADSRSSGTAVGGALTTLSLLVGLAGMYFAGRMIDRGARA
                     SIVFMLGAALQAPFLLIVGSAGPAPLVPLFMAVAFFHFFTQPAGNHLVAEFTPPRLRG
                     LGYGIYFLMTFGAGSLGAVIAGWVSENVGLQSVFPVLAGLLLPSIVAILLVSGVGRRG
                     REPTLSAGGPRREAR"
     gene            4652..5182
                     /locus_tag="VD788_03840"
     CDS             4652..5182
                     /locus_tag="VD788_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75426.1"
                     /translation="MKRARRLGLMTTYVLLAGLPAVATAQEVATERELARELAGIRVS
                     LDRLVKLAESQLHLQRTELLIRRINLSEQRIEPLERRLRDAEATLSAREDELERLRAV
                     VAERESALDEAVREGRTEEEGELRRMLREMDAVLRSEQPLLDTAYQRVRQLEDEIAEE
                     REGIRTLDERLAELLD"
     gene            complement(5089..6054)
                     /locus_tag="VD788_03845"
     CDS             complement(5089..6054)
                     /locus_tag="VD788_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXV75427.1"
                     /translation="MGSARVALLVAVVIWGWTFVATKVCLEQLSVAELLGLRLLVALP
                     VLGAIARGRGTPLFPRPVPRALVGGTLLITIHFLIQISGLRLTTATRTGWIIAITPVV
                     TAVLSALVLRERVTRRLVCGIAVATCGILLLLSGGRPGGLAWSGAAGDWLILASAHTW
                     ALYTIAIRNITRTTAPLSVTIGVLAPPAALLGLYALSRFDAARIVGLRVETICALLFL
                     GVFGTALAHWLWQVGVSRVGAARAGVFLYLEPLSTTALAVPYLGETFGPSTLAGGAMV
                     LLGVWIAERPSGSGGQSSNSANRSSSVRIPSRSSAISSSSCRTRW"
BASE COUNT          916 a         2108 c         2181 g         1048 t
ORIGIN      
        1 gaccgaactg ccactcgacg tcggcggctt cgtcgtttcg cccgatggtt cacggctcgc
       61 actgtcgctc gacgtgttcg tcgactgcga cacgccggag tgcaccagca agcgactgga
      121 aggccgccgg caggagaagg cgagcggacg gatctacgag cggttgttcg tccggcactg
      181 ggacggctgg aaggatggac ggcggtctca tctgttcgtc gtatcgctcg acgatggaac
      241 cgcggtcgac gcgatgcgcg ggatggatgc ggattgtccg agcaagccgt tcggcgggcc
      301 ggaggagtat gcctggacgc ccgacggaag cgagatcgtc ttcacggcgc gcgacgtcgg
      361 gcgcgaggag gcctggtcga ccgatttcga cctgttccgc acgccggccg acgggtcgtc
      421 ggcaccgacc aagatcacca gcgcgaaccg ggcgtgggac acccaaccgg tcttttcgcc
      481 ggacggggcg acgctggcct atctggcgat gagccgcccg ggctacgagt cggatcgctt
      541 ccggatcgtg ctgatggact ggccggcggg cgaaccgcgg gtgttgaccg agggctggga
      601 tcgctcgccc ggttcgatcg gctggtcgcc cgaccgaaag acgatctacg cgaccgccgc
      661 gaacctggga cagagctcgc tgttcgcgat cgacgtcgac gacggagcgg tccgcaccct
      721 ggtcgagacc ggtaccgtcc gggcgatcct gccggcggcc gaacgcatcg tcttcgcgcg
      781 cgacgagcta cgacggccgg tcgagctgtt ctcgattcgg gtcgacggcg gtgacgagca
      841 acgcctgacg cacatcaacg acgattacgt cgccgccgca cggatggggg agcccgagca
      901 gttcgacttc gccggctgga acggcgagag ggtccacggc tacgtcgtca aacccgtcga
      961 cttcgacccg aaacggcgct acccggtcgc cttcctgatc cacgggggac cccagggctc
     1021 gttcggcaac gacttccact accgctggaa cccgcaggcc tacgccggcg ccgggtacgc
     1081 ggtcgtgatg atcgacttcc acgggtcggt cggctacggc cagcgcttca ccgactcgat
     1141 caacggcgat tggggtggca agccgctcga cgacctgcag caggggctgg ccgccgcgct
     1201 cgcgcgctat ccgttcatgg atgccgatcg catctgcgcg ctcggtgctt cctacggcgg
     1261 ctacatgatc aactggatcg ccggcaactg gcccggtcga ttccgctgcc tggtcaacca
     1321 cgacggcaat ctcgacgagc gaatggcgta ctacgacacc gaggagctgt ggtttccgga
     1381 gtgggagcgc ggcgggccgc cgtgggaggt tccgcaggcc tacgccgagc acaatccgat
     1441 cgaccacgtc gccgagtgga agacgccgat gctcgtgatc cacggggcgc tggactaccg
     1501 cgtcgtcgat acacagggtc tgtcgacctt caccgcgctg cagcggcgcg ggatcccgag
     1561 caagctgctc tatttcccgg acgagaacca ctgggtcctc aagccgcaca acagcatcct
     1621 gtggcacgaa acggttctcg actggatcga tcgatggacc gagccgcgtt agagggccga
     1681 cgatgacccg gcgccgcggg caggggcggg acctgctgct cgcgatcttc ctggtgtcgg
     1741 ctctcgccgc gctgatctgg ccgatctacc ccctcgtcgg ttcgtcgatc gagccgcggg
     1801 tgctcggcct gccgttctcg ctggcgtggg tcgtgatctg gctcgtcctg tcgtttttgg
     1861 cgatgtggat ctaccacgtg agcggcagcg acgaacgcgg ttgacatgcc gtcgtgggtc
     1921 atcgtcttcg cggtcgccgt cgtctacctg gcgctgagcc tgctggtcgg gatgcacccc
     1981 gggcgccgcg cgaccaagac tgcatccggg ttcgtcgcgg gggatcgcag tctcgggttg
     2041 ttcgtcatgt acttcatcac cggggcgacg atcttcagcg cgttcgcgtt tctcggtgct
     2101 ccggggtggg tttactcacg cggcgcggcc gcgttctaca tcctggccta cggctgtctg
     2161 gcgttcctgc cgttctattt cctcggcccg cgggcggccc ggctgggccg ccggttcggc
     2221 ttcgtcaccc aggcggagat ggtcgccgaa cgatacgggt cacgcgcgct cgcggggctg
     2281 atggcgctgg tcagcgtgct cggtttcatc ccgtatctcg ccctgcagat gaagggagcg
     2341 ggctacgtac tggccagcgt gacgcgcggg cacgtccccg agtggctcgg tgccgcgatc
     2401 gtctacggcg tcgtcctgat ctacgtcatg cgcagcggcg tgctcggcgt tggctggacc
     2461 aacacgttcc agggcatctt gatgatggga ctggcgtggg ggctcgggct ctacctgccg
     2521 tacgagctct atggcggggt cggtccgatg ttcgacgcga tcgccgagac gcggcccgag
     2581 ctcctacgcg ctcccggtct tgcggcatca ggggagagct ggtcctgggg cgagtacggc
     2641 tcggcggtcg tcatctcgat cgtcgggttc tcggtctggc cgcatctgtt catgaaggcg
     2701 ttcaccgccc gggacgaccg gacgattcgt cgcacggtgg tgctctatcc gacgttccag
     2761 ctgttcctcg ttccgctgtt cctgatcgga ttctccggcg tcctgttcaa tccggccccg
     2821 tccagcccgg accagatcct gccgcacatg ctgatgcaga tggagctacc cgccgtgctc
     2881 gtcggcctgt tctgtgcggg cgctctcgcc gcctcgatgt cgtccggtga cgcgatggcc
     2941 cacgccgcgg cgtcgatcgc ggtgcgcgac ggagtggtga cgactctcgg cgtgtcgctc
     3001 gacgagcggc agcagcgcga ctggatccgg gtcggcgtcg tgctgtcgat gatcgcagcc
     3061 tacgtcctga ccgtctcgta cgacaaggac ctcgtcgcgc tcttgctcta cgcctacggg
     3121 ccgatcggtc agttcgcgcc ggtcgtcttc gccacgctgt acagccggcg ggcgacgggc
     3181 gccggtgtat tcgccggact gctcgccggt tcgctcgtca ccatcgtcct cgacgtccgt
     3241 cccgactgga ggccgtggcc gatccacgcc ggcgtctacg gtctggtcgc gaacctcgtc
     3301 acgctggcga tcgtctccgc cgcgacgccc caacgccgcg acgcggggcg cgaggatcgg
     3361 tacctcgacg tggccgccgg tcggagcacg gatgccgccc ccgccgccct gcgctagaat
     3421 ccggcgctga attgggcgct cgcttcaacc tccgcacgtt ggaccgcggc cagcgtcgcg
     3481 tgctcggcac gacctgcgcg gcacacgcgc tgatccacgt ctacgagctg agtctccccg
     3541 cgctgttgtt gctgatccag gacgagttcg acgcgggcga tctcgaaatg ggcggcatcg
     3601 tgatgctgtc cgccctgctg ttcggtctgg gggcgctgcc ggccgggtac ctcgtcgacc
     3661 ggctgggatc gaagccgttg ctcgtcggct gcctgtgggg cggatccctg tcgctcggcg
     3721 cgatggcgtt cagcccgagc gcgccgtcgt tcgccgtcgc cgcggcgctg ctcggcctgg
     3781 cactcagcat ctaccatccg gccgggacgg cgttgatcac ccacgccatt ccgctctccg
     3841 gcaaggtgtt cgcactgcac gggatggcgg gcaatctcgg tgtggcgggg gccggcgtcg
     3901 tggcgggcac gttgggcgcc gcgttcggct ggcgctgggc ggtcgcgctg ctcgccggct
     3961 gcggactgct gctcggcggg cgggcgttgc tgttgccggc gcccgcgctg caggagatcc
     4021 gcgaccgttc gggccgcgga agctggcacg ccttcgccgg gttactggtc gcgacgatgt
     4081 tcatggggat ggtctaccga ggactcacga cgtttctacc caagttcttc gcggtcagct
     4141 tcgccgacag ccgcagctcg ggaaccgccg tcggcggggc gttgaccacc ctctccctgc
     4201 tcgtcggtct cgccggcatg tacttcgcgg ggcggatgat cgatcgcggc gcgcgggctt
     4261 cgatcgtgtt catgctcggc gcggcgctcc aggcgccgtt cctgctgatc gtcggctcgg
     4321 ccgggccggc cccgctcgtc ccgttgttca tggcggtcgc cttcttccac ttcttcaccc
     4381 agccggccgg caaccatctg gtcgccgagt tcacaccgcc ccggctgcgg ggtctgggat
     4441 acggtatcta ctttctgatg accttcggcg cgggctcgct gggagcggtc atcgccggat
     4501 gggtcagcga gaacgtcggc ttgcagtcgg tcttcccggt gctggccggc ttgctgttgc
     4561 cgtcgatcgt cgcgatccta ctggtgtccg gcgtcgggcg gcgaggccgg gagccgacgc
     4621 tatctgcggg cgggccgagg agggaggcga gatgaaacgg gctcgacgac tgggtctgat
     4681 gacgacatac gtgctgttgg ccgggttgcc cgcggtcgcg accgcgcaag aggtcgcgac
     4741 ggaacgcgag ctcgcacggg agctggccgg aatccgggtc tcgctcgatc gcctggtgaa
     4801 gctggccgaa tcccagctcc acctgcaacg aaccgagctg ctgatccggc ggatcaacct
     4861 cagcgagcag cgcatcgagc cgctcgagcg ccggttgcgc gacgcggaag cgacgctgag
     4921 cgcgcgggag gatgagctcg agcggctacg cgccgtggtc gccgaacggg agtcggcgct
     4981 cgacgaggcc gtgcgcgaag gccgcacgga ggaagaaggc gaactgcgcc ggatgctgag
     5041 agaaatggac gccgtgctcc gctcggaaca gccgctgctc gacactgcct accaacgggt
     5101 ccggcagctc gaggacgaga tcgccgagga gcgggaggga atccgaacgc tcgacgaacg
     5161 gttggccgag ttgctcgact gaccgccgct cccgctcggg cgctcggcga tccacacgcc
     5221 gagcaacacc atcgcgccgc cggcgagcgt cgatgggccg aacgtttcgc cgagataggg
     5281 cacggccagc gcggtcgtcg acagcggctc gaggtagaga aacacgccgg cccgcgcggc
     5341 gccgacgcgt gacaccccga cctgccacag ccagtgggcg agcgccgtgc cgaacactcc
     5401 gagaaacaag agtgcgcaga tcgtctcgac ccgcaggccg acgatccgcg cggcgtcgaa
     5461 ccgagacagc gcgtacagcc cgagtagcgc cgccggcggt gcgagcacac cgatcgtgac
     5521 ggacaagggc gcggtcgtgc gcgtgatgtt gcggatcgca atggtgtaga gcgcccaggt
     5581 gtgggccgag gcgaggatca accagtcgcc ggccgctccg gaccaggcga gtccgcccgg
     5641 acggccaccc gacagcagca gcaggatgcc gcaggtggcc accgcgatcc cgcagacgag
     5701 ccgtctcgtc acccgctcac gcaggacgag cgcggacagc accgccgtca cgaccggcgt
     5761 gatcgcgatg atccagccgg tgcgggtcgc ggtcgtgagt cgcagcccgc tgatctggat
     5821 caggaagtgg atcgtgatca gcagcgtgcc gccgaccaac gcgcgcggca ccggtcgggg
     5881 aaacagcggc gtgccccgcc cgcgggcgat cgctcccagc accggcagtg cgacgagcag
     5941 tcggaggccg agcaactcgg ccacgctcag ttgttcgaga cagaccttgg tcgcgacgaa
     6001 cgtccagccc cagatcacga ccgcgaccag caaggcgacc cgcgccgatc ccatcgctcg
     6061 ataccccacg tcgcgctcag aacagaccga gactccaact cgtcgcgagc gcccagccgg
     6121 cgccgctcgc gaacagcttg ggtgagccga ggatgacgtc gaccggagac ccccccgcgt
     6181 cgtcgaggta aaccagccgc agatactcca gcaccccgaa caacacgaac ggaagcgagg
     6241 cgaactcgcg tcc
//
LOCUS       DASPGN010000140         3122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_140398, whole genome shotgun sequence.
ACCESSION   DASPGN010000140 DASPGN010000000
VERSION     DASPGN010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140398"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3122
                     /locus_tag="VD788_03850"
     CDS             <1..>3122
                     /locus_tag="VD788_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75428.1"
                     /translation="VGAVTFSDIENLGGNVGDDDFVFADGASVTGGIDGGGGVNTLDY
                     SAYTAGNVVTVSLGSGAVTGLGSVASIQEVLGGQGTGDTLIGPDADAEWHITGENAGT
                     VLGISFAGFENLTGAAGNEDAFIFRPGGSLAGTLDGGPGGLDALAIEDPSSSDPDPKV
                     LTAVVPDASGAGTQPFGDIYPGDARSVTYQGMDPYFLNAGGPADALIRTSVFGETLIL
                     EDGPGPTELQLRVTSAVLGTPFGSIELWNPLTSALIGPIVFEIPTSSLTIQTKAGNDS
                     LTVTTTRLDNVADLDVRLEAGNLVAGSLDKDEIRLSSDILTRGGDLEVIAETIVVSAG
                     TILSTRDAVGDQATAVSLGASGDMDFIGHSISVEDGAQLLSFHSSGAPDAAGLSADVV
                     VESVELERDNIFGETRKIWEPGRSYIDVKTVAGAGSAGSGLTLDIITDTQGNPSFRIH
                     ERGSGYAVGDTVQVRVVDLKDASLNFLESKLLELFGGAEDTVIATLEVSELVVEGGDI
                     RLSAIHKTNAVGTVFGVQLFPEKAHVSAEITIGAALIHGRDVILRAEADNNDPFNGEL
                     EAGKQKFDGVLDFLSEVRPLVGISDVSATATITIGDGAWILADADVVIEARADSAAEI
                     RTLGIVGGVGYARSEADATVTVQGGATLAAAGDVAVRSIANNTVTANVVTSNLVARIP
                     VDIAVGVTEAYSNAVTRVEEGAVIVAGGRADIWAIHARDLASEVSTSANFGMLGVAVL
                     VSLSEANSLAEVAGTVTAADDVSVIAQTVSTQNQSQVASTIGLGFVDKLKKRITNPVA
                     DPVKGWLKGLFSGKKNTDNIPQSMKNSKFGLTAAVVVADHESSARATIATGAVVRSEG
                     AVSVLADVVDRPVLSAESSIKKGFDRIAKTNFGIQKLGSVSAAVEIGTYTNEAQASIA
                     SGAQVDAQRGIVVAAHTLLPQDAPWANFFADDGAGTGAAIGDTLLDLFIGTEKAFATT
                     VAKVSSDAKKVGLAGAVTVFNNRASAQASIEDGALINQNPDALWMLDQQDVTVSADTS
                     VESVNLSGNL"
BASE COUNT          554 a         1021 c         1059 g          488 t
ORIGIN      
        1 ccgtgggcgc agtcactttc agcgatatcg agaacctcgg cggcaacgtc ggcgacgatg
       61 atttcgtctt cgccgacggc gcgagcgtca cgggcgggat cgacggcggc gggggcgtca
      121 acacgctcga ttactccgcg tacacggcgg gcaacgtggt cactgtgagc ctcggctccg
      181 gcgcggtcac gggtttggga agcgtggcga gcatccagga agtcctgggt ggtcagggca
      241 ccggggacac cctcatcggg cccgacgccg atgccgagtg gcacatcacc ggcgagaatg
      301 cagggacggt tctgggcatt tcgttcgccg gcttcgaaaa tctcaccggc gcggcgggca
      361 acgaggacgc gttcatcttc cgtccgggcg gcagcctcgc cggcacgctc gacggcggcc
      421 caggaggcct cgatgctctt gccatcgagg atccctcgag cagcgacccg gatccgaaag
      481 tactgacagc cgtcgtgccc gatgcaagcg gggcgggcac ccagccattt ggtgacatct
      541 acccgggcga cgcgcgcagc gtcacgtacc agggcatgga cccatacttc ctcaacgccg
      601 gcggcccggc ggatgcgctc atccgcactt cagtcttcgg cgagaccttg attctcgaag
      661 acggtccggg gccgaccgag cttcagcttc gcgtcacctc ggcggtcctc ggcacgcctt
      721 tcggctcgat cgagctctgg aatccgctca cgagcgccct gatcgggccg atcgtgttcg
      781 agattcccac cagctcgctc accatccaga ccaaggccgg caacgactca ctgacggtga
      841 cgacgacgcg gctagacaac gtcgccgatc tcgacgtcag gttggaggcg ggaaacctcg
      901 tggccgggag cctcgacaag gacgagatcc ggctctcgag cgacattctg acgcgcggcg
      961 gcgacctcga ggtgatcgcg gaaacgatcg tcgtgtccgc cggcacgatc ctgtcgacgc
     1021 gcgatgcggt cggggatcag gcgacggccg tatccctcgg ggcttcgggc gacatggatt
     1081 tcatcgggca cagcatctct gtcgaggatg gcgcccagct cctgagcttc cacagctccg
     1141 gcgcgccgga tgcggctgga ctgagcgccg atgtggtggt cgagagcgtc gagctggaga
     1201 gagacaacat cttcggagag acgcgcaaga tctgggagcc ggggcgctcg tatatcgatg
     1261 tcaagaccgt cgccggcgcg ggcagcgcgg gctcgggcct gaccctcgac atcatcaccg
     1321 acacgcaggg caatccgagc ttccgcatcc atgagcgcgg cagcggctac gcggtcgggg
     1381 atacggtcca agtccgcgtc gtcgacctga aggacgcgtc gctgaacttc ctggaatcca
     1441 agctgctgga gctcttcggc ggcgccgagg acacggtcat cgccactttg gaagtgagcg
     1501 aactcgttgt cgaaggcggc gacatccgcc tgtccgcgat ccacaagacc aacgccgtcg
     1561 gcacggtatt cggcgtgcag ctctttccgg agaaggccca tgtcagcgcc gagatcacga
     1621 tcggcgcggc cctgatccac gggcgcgacg tgatccttcg cgcggaagcc gacaacaacg
     1681 acccgttcaa cggcgagctc gaggccggca agcagaagtt cgatggcgtc ctcgacttcc
     1741 tgtcggaggt ccgcccgctc gtcggcatca gcgacgtttc cgcgacggcc accatcacca
     1801 tcggcgacgg ggcgtggatc ctcgccgatg cggacgtggt gatcgaggca cgggccgatt
     1861 cggcggccga gatccgcacc ctcgggatcg tcggcggagt cggctacgcg cgctccgagg
     1921 ctgacgcgac cgtgacggtg cagggcggag caacgctcgc cgccgccggc gacgtggcgg
     1981 tcagatcgat cgccaacaac accgtcaccg ccaacgtcgt cacctcgaac ctcgtcgcca
     2041 ggatcccggt cgacatcgcg gtcggcgtga ccgaggcata ttcgaacgcc gtcacgcggg
     2101 tcgaagaggg cgccgtcatc gtcgccgggg gtcgcgccga catctgggcg atccacgcca
     2161 gggatctcgc ctccgaggtc tccacgtccg cgaacttcgg gatgctgggc gtggccgtcc
     2221 tcgtctcgct ctcggaggcg aactcgctcg ccgaggtcgc gggaacggtg acggcagcgg
     2281 acgacgtctc ggtgatcgcg cagaccgttt cgacgcagaa ccaaagccag gtcgcgagca
     2341 cgatcggcct gggctttgtc gacaagctca agaagagaat caccaatccg gtagccgacc
     2401 cggtgaaggg ctggctgaaa ggcctgttct cggggaagaa gaacaccgac aacattcccc
     2461 agtcgatgaa gaacagcaag ttcgggctga ccgcggcagt ggtcgtcgcc gatcacgaaa
     2521 gctcggcgcg cgccacgatc gcgaccggcg ccgtggtgcg ctccgagggc gcggtgtcgg
     2581 tgctcgccga cgtggtcgat cgcccggtgc tgtccgcaga aagctcgatc aagaagggct
     2641 tcgaccggat cgccaagacg aacttcggca ttcagaagct cggctcggtg agcgcggcgg
     2701 tggagatcgg aacctacacc aacgaggcgc aggcctcgat cgcctccggc gcgcaagtcg
     2761 acgcgcagcg cgggatcgtg gtcgccgcgc atacgctgct gccccaggac gcgccgtggg
     2821 ccaatttctt cgccgacgac ggcgcgggca cgggcgcggc gatcggcgac acgctgctcg
     2881 acctcttcat tggcacggag aaggcgttcg ccacgactgt cgccaaagtc tcctcggatg
     2941 ccaagaaggt cgggctcgcc ggggcggtga cggtgttcaa caaccgggcg tcggcgcagg
     3001 cctcgatcga ggacggagcg ctgattaacc agaatcccga tgctctctgg atgctcgatc
     3061 agcaggacgt gaccgtctcg gcggatacga gcgtcgagtc ggtcaatctc tcgggcaacc
     3121 tg
//
LOCUS       DASPGN010000141         9993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1428574, whole genome shotgun sequence.
ACCESSION   DASPGN010000141 DASPGN010000000
VERSION     DASPGN010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9993
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428574"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VD788_03855"
     CDS             complement(<1..290)
                     /locus_tag="VD788_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXV75429.1"
                     /translation="MPESSHIPRKRQFKTNEVCQLTDTQPYVLRFWESEFPQLNPDRR
                     GGQPLYRREDIDVVMRIKRLLYDEEYTIEGARRRLEQELNGELEPEPVPSRPV"
     gene            complement(367..711)
                     /locus_tag="VD788_03860"
     CDS             complement(367..711)
                     /locus_tag="VD788_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV75430.1"
                     /translation="MDKPWGYELRFVCTERYAGKILFVKAGSQLSLQYHECKDEAFFV
                     QDGTLELVLGRGPEQRVERLGPGQSRHIRPGTVHRFRAVTDCTLFEVSTPELDDVVRL
                     EDDFGREGTSEA"
     gene            complement(722..2455)
                     /gene="ptsP"
                     /locus_tag="VD788_03865"
     CDS             complement(722..2455)
                     /gene="ptsP"
                     /locus_tag="VD788_03865"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HXV75431.1"
                     /translation="MSRRFHGIGVSPGIAIGRVYLLHAEPLPVVPDPIPPERVEDELS
                     RFAQARELARGELEELKRRVRETLGDHYAGILDAQLLILDDRELIERTRGRIRVGRVS
                     ARWALKQVVEEFDRKFAEMDEAYLSEKGGDLADVHLRLQRLLRGAVRHRDIPDGPRIV
                     VAHGLGPSDAADLARRGVAGLATDVGGRTSHTAILAQALGVPAVAGLRLASQHATPGE
                     TVVLDGERGELLLDPTDEQRAAAMARRMSWLDDEAQAARDRAVPVRTRDGVEIAIRAN
                     IEFPSETDNVRRFGAQGVGLYRSEFLFLSHAPELPGEDDHYRTYRDLARAVAPHPAVI
                     RTLDLGGEKYFHEVLQHEEGNPVLGLRAVRFCLERPEIFRPQLRGLLRAAVEPNLKIM
                     LPLVTSVEEVRQVRALIGAEAAELTARGVPARSDVPVGVMIEVPAAALAADLIVPHAD
                     FISIGTNDLIQYALAVDRGNELVNYLYQPDHPGVLRMLRFVIDEAGRAGVPVAVCGEM
                     ATDPRSLAWLIGLGLREVSMQPRSIGRAREMVSEIDSAEAEAFARRLSRDGATDDADR
                     PFETAKPSGEP"
     gene            complement(2458..2727)
                     /locus_tag="VD788_03870"
     CDS             complement(2458..2727)
                     /locus_tag="VD788_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXV75432.1"
                     /translation="MSAVEVQILNRLGLHARAAAKMVHVANRYKSAITVSRTDSRVDG
                     KSILGLLTLAASQGTRLTLSADGADANEALAALSTLVRERFGEDE"
     gene            complement(2724..3143)
                     /locus_tag="VD788_03875"
     CDS             complement(2724..3143)
                     /locus_tag="VD788_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXV75433.1"
                     /translation="MLVVTHGRLAEELVHAARTIVGGIERLEAVSIDWDDDVGEATKR
                     IEKALERVNDGRGVVLLTDMFGGTPTNLGIALHDPGRVEVVTGVNLPMLIKFANLRDP
                     ISLAEAAVMIADQGREAIHVASRVLESRGPHRSGEAS"
     gene            complement(3179..4048)
                     /gene="rapZ"
                     /locus_tag="VD788_03880"
     CDS             complement(3179..4048)
                     /gene="rapZ"
                     /locus_tag="VD788_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HXV75434.1"
                     /translation="MAVHRLVVVTGMSGSGKSLAANCFEDLGYFCVDNLPVQMIPPFC
                     ELIQRGAENIPLAALVVDARERGFLEQVPEILKGLRAADIPLEILFFDCSDDVLKRRF
                     SETRRPHPMTTPGGTLEQAIHAERAVLRPLRDVSSRVIDTSNLTAHQLRSFLTGAYGT
                     DERISTPNVNVESFGFKYGVPAQVDLLFDVRFLPNPYFVDELRHMTGLDRPVREFLER
                     SELTGQFVERLQDFIDYLIPLYRAEGKAYLTIGIGCTGGKHRSVALAERLGRHLAAQG
                     LAHSVSHRDVAKE"
     gene            complement(4049..5080)
                     /gene="hprK"
                     /locus_tag="VD788_03885"
     CDS             complement(4049..5080)
                     /gene="hprK"
                     /locus_tag="VD788_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HXV75435.1"
                     /translation="MGEHESCAIPSMAVRELLTDEAAEGIHLSLVSGAEGLDNIVNQP
                     RIQKPGLALAGFLEYIHPGRIQILGKSEFTFLAERTPGERSRILSQLCREGGSCFVLT
                     SNLEPMREMIEETERHNIPLLRTSLTSSTTIDKLTRFLEDRLAPRVVLHGVLLDVYGL
                     GVLLFGDSGVGKSECALDLVVRGHRLVSDDVVEIRRKGNMLLGTGPELTRYHMELRGV
                     GIVNVKDLFGVAAVRMNKYVEYVIRLDPWRAGKKYDRLGLDEQTYEVLGIELPYVEMP
                     VGPGRNLSVLIEVAARNHLLKLKGYHPARELARRLGEKLHPHAYDPDDEDEEVRAAVR
                     RGRPEEGES"
     gene            5274..6347
                     /locus_tag="VD788_03890"
     CDS             5274..6347
                     /locus_tag="VD788_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75436.1"
                     /translation="MSSRPIVHVIGTGTIGEPLIGLLSIMKDDLGIEEVTFHKRTPLL
                     TDRSKVFGLINKGAKLAVDDGAARGFQDLGMEPALETKEALQRAAVVIDCTPSGVGTQ
                     NKTEFYENYTSNTLGFIAQGSEFGFGKPYARGINDRALVRGKDQFIQVVSCNTHNLSI
                     LIDTLALEDGGPPNLNEGRFVCMRRANDISQDSSFVPSPQVGRHKDARFGTHHARDAW
                     HLFQTLGYDLNLFSSALKLNTQYMHTIFFDIRVNNPTTVDSLQERIHANDRIAVTYKT
                     SANSVFSFGRDQGHYGRILNVCVVALPTLMVRNEKEILGYCFTPQDGNSLLSSISAAT
                     WFLYPDDYEDRIQCVAEYFHDEV"
     gene            complement(6355..7338)
                     /locus_tag="VD788_03895"
     CDS             complement(6355..7338)
                     /locus_tag="VD788_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXV75437.1"
                     /translation="MANVLVFVERREGEIKRPSLQVLTEGRRLAAAGSGRVDALVLGP
                     GAAAAAASLARYGAERVFHVEDPSVELYSPELYSACLVEATGRAEADFVLLAATAMGK
                     DLAARTAARLDTACASDVVEIEVDRSGGLRAKRPVYSGKAVARVVLKARPAVATLRPN
                     VFAASEAAAPAAGAAEALGLPLDPAAARVTTRELRKPDRQELDVAEASIIVSGGRGLK
                     DPKNFSLVRELADALGGAVGASRAVVDAGWIAHSHQVGQTGKVVSPTLYIAAGISGAI
                     QHLAGMSSSAVIVAINKDPDAPIFKVADYGIVGDLFDVLPALTRAVRELKN"
     gene            complement(7341..8129)
                     /locus_tag="VD788_03900"
     CDS             complement(7341..8129)
                     /locus_tag="VD788_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXV75438.1"
                     /translation="MRIVVGIKPVPDTETKIKVGPDGFAIDPAAVTKWIISPYDEFAL
                     EQALQLKERGVASEVVAVSAGRDSAQQVARQALATGADRAVWIQDPRFDLADGLTRAK
                     ALAAVVREEGAGLVLVGKYGVGTDEGQTGAMLAELLAWPNTFAVSKLELVDGRFTAEH
                     EVEGAVEVVDGSLPAVISCEKGLNDPRYPSLKGIMQAKKKPLDLRTAEQCGVGDAELE
                     GEKKLAWEALELPPERASGRLIDGDAEQAARELARLLHQEAKVI"
     gene            complement(8161..9402)
                     /gene="fabF"
                     /locus_tag="VD788_03905"
     CDS             complement(8161..9402)
                     /gene="fabF"
                     /locus_tag="VD788_03905"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXV75439.1"
                     /translation="MTRRVVITGIGLVTPVGLDTTSSWQALLAGKSGIDRITKFDASN
                     LSSQIAGEVKGFDPTAYIEPKEARKMDSFSHYAVAAAKQALDDAGFTIDDGNAERVGV
                     YIGSGIGGLPLLESTYRQILERGPRRTSPFFIPGMILNLAAGHVSILFGAKGPNLALA
                     TACATGSHAIGESYRMIREDYAETMIAGGTEAVITSLAVGGFCAMKALSTRNDEPERA
                     SRPFDAQRDGFVIGEGAGILLLEERHAALARGARIYAEITGYGVSGDAYHVSAPSIDG
                     DGPVRAMRMALDRAGVAPGDVDYINTHGTSTPAGDRIEVMAIKKLFGKHAYQLVAGST
                     KSMTGHLLGAAGGLETAVAALAVFHDRVPPTINQEFPDPECDLDCAPNEARSMTVRTA
                     LNNSFGFGGTNATVLLAKHEA"
     gene            complement(9476..9715)
                     /gene="acpP"
                     /locus_tag="VD788_03910"
     CDS             complement(9476..9715)
                     /gene="acpP"
                     /locus_tag="VD788_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXV75440.1"
                     /translation="MASVEEKVKHIIVEQLGVDEDEVKPEASFVDDLGADSLDVVELV
                     MALEEEFGLEISDEDAEKLRTVKQAVDYIGANAKD"
     gene            complement(9777..>9993)
                     /locus_tag="VD788_03915"
     CDS             complement(9777..>9993)
                     /locus_tag="VD788_03915"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV75441.1"
                     /translation="NVTVNCVAPGFIDTDMTRALDDTQRQALLDRIPLGRLGTVDDVA
                     HCVRFLVGGGADYITGTTIHVNGGMYM"
BASE COUNT         1638 a         3456 c         3186 g         1713 t
ORIGIN      
        1 accggccggg atgggacggg ctcgggctcc agttcgccgt tcaattcttg ctcgagcctc
       61 cgccgcgccc cctcgatcgt gtactcctcg tcgtacagca gccgcttgat tcgcatgacg
      121 acgtcgatgt cctctcgacg gtagagcggc tgcccgccgc gccggtcggg attgagctgg
      181 ggaaattcgc tctcccagaa tcgcaacacg tacggctggg tatcggtcag ctggcagacc
      241 tcgttggtct tgaactggcg cttgcgcgga atgtggctcg attcaggcac gggcttctcg
      301 cggcatggtc acggtcaccg aggcagccct actataagcc gtgcccaccg ctcgcggcaa
      361 gcccggtcag gcctcgctcg tcccttcgcg gccgaagtcg tcctcgagcc ggacgacgtc
      421 atccaactcc ggggtcgaga cctcgaacaa cgtgcaatcg gtcacggcgc gaaagcggtg
      481 gaccgtcccg ggcctgatgt ggcgcgattg gccaggtccg agtcgttcga cccgttgctc
      541 gggaccccgt cccagcacca attcgagtgt cccgtcctga acgaagaagg cctcgtcctt
      601 gcactcgtgg tactgcaagc tcagctgcga gccggccttg acgaacagga tcttgccggc
      661 gtaacgctcg gtgcagacga agcgaagctc atagccccag ggtttgtcca cgcgattcat
      721 ctcaaggctc ccccgatggt tttgcggtct cgaagggtct atctgcatcg tcggtcgcgc
      781 cgtcgcgcga caagcgccgc gcgaacgcct cggcctcggc gctgtcgatc tccgacacca
      841 tctccctggc ccgaccgatg cttcgcggct gcatggaaac ttcgcgcaat ccgagaccga
      901 tcagccaagc gagcgagcga gggtcggtcg ccatctcgcc gcagacggct accggtacgc
      961 cggcgcggcc agcctcgtcg atcacgaagc gcagcatgcg cagcacgccg ggatggtccg
     1021 gctgatagag atagttgacc agctcgtttc cgcggtcgac ggccagcgcg tactgaatca
     1081 gatcgttcgt tccgatgctg atgaagtcgg cgtgcgggac gatcaggtcg gcggccaaag
     1141 cggccgcggg cacctcgatc atcaccccga ccggaacgtc ggagcgcgcg ggcacgccgc
     1201 gagccgtgag ttcggccgct tccgccccga tcagcgcgcg gacttgccgt acctcctcga
     1261 ccgaggtgac gagcggcagc atgatcttga ggttgggctc gaccgcggcg cgcagcagtc
     1321 cgcgcagttg cgggcggaat atctcaggcc gctcgagaca gaagcgcacg gcccgcagcc
     1381 cgagcacggg gttgccttcc tcgtgctgca ggacttcgtg gaaatacttc tcgccgccga
     1441 ggtcgagcgt gcggatgact gccgggtgcg gggcgaccgc ccgcgcgaga tcgcggtagg
     1501 tccgatagtg gtcgtcctca ccgggcaact ccggggcgtg actgaggaac aggaactcgg
     1561 agcggtaaag gcccaccccc tgcgcgccaa accgtcgcac gttgtcggtc tcgctcggga
     1621 attcgatgtt ggcccgaatc gcgatctcga cgccgtcacg cgtccttacc ggcaccgctc
     1681 ggtcgcgggc ggcctgcgct tcgtcgtcca gccacgacat tcgccgcgcc atcgccgccg
     1741 cccgttgctc gtcggtcggg tcgagcagca actcgccccg ttctccgtcg agcactaccg
     1801 tttcccccgg cgtggcgtgc tgactggcga ggcgcaagcc ggcgaccgcc ggcacgccga
     1861 gcgcctgggc caagatcgcc gtatgcgagg tccgtcctcc gacgtcggtg gccaggccgg
     1921 cgactccgcg gcgcgcgaga tcggccgcgt ccgacggccc gagcccgtgc gcgaccacga
     1981 tccgcggccc gtcggggatg tcgcgatggc ggaccgcgcc ccgcagcagt cgctgcaggc
     2041 gcaggtgcac gtcggccaga tctccgccct tctcgctcag ataggcctcg tccatctcgg
     2101 cgaacttgcg gtcgaactcc tcgaccacct gcttgagcgc ccaacgcgcc gagacccggc
     2161 cgacgcggat gcgtccccgc gtccgctcga tcagctcgcg gtcgtcgagg atcaacagct
     2221 gcgcgtcgag aatcccggcg taatggtcgc cgagcgtctc ccgcacacga cgtttcaact
     2281 cctcgagctc gccgcgggcc agctcgcgtg cctgcgcgaa ccgcgacagc tcgtcctcga
     2341 cccgttccgg tgggatcgga tccggcacga ccggcaacgg ctcggcgtgc agcagataga
     2401 cccggccgat ggcgatgccc ggagagacgc cgatcccgtg aaatcgtcgt gacatcctca
     2461 ctcgtcctcg ccgaaccgtt cgcgcaccag cgtcgacagc gctgccagcg cttcgttcgc
     2521 atcggctcca tcggcggaca acgtcagccg cgtgccctgc gaggcggcca gggtcaacag
     2581 cccgaggatc gacttgccgt cgacccgcga atcggtgcgg ctgaccgtga tcgcgctctt
     2641 gtagcggttc gcgacgtgca ccatcttggc tgccgcgcgc gcgtggagcc ccaaccggtt
     2701 gaggatctgg acctcgaccg cgctcaactc gcctcccccg accggtgcgg cccccgcgac
     2761 tcgagcacgc gcgaggcgac gtgaatcgcc tcgcgcccct ggtcggcgat catcaccgcg
     2821 gcctcggcca acgagatcgg atcgcgcagg ttggcgaatt tgatcaacat cggcaggttg
     2881 actccggtga cgacctcgac gcgcccgggg tcgtgcaggg cgatgccgag gttggttggc
     2941 gtgccgccga acatgtcggt caacagcacc acgccgcgac cgtcgttcac ccgctcgagc
     3001 gccttttcga tccgtttggt cgcctcgccg acgtcgtcgt cccaatcgat cgagaccgcc
     3061 tccaggcgct cgatgccccc gacgatcgtt ctcgccgcgt gaactagctc ctcggccagg
     3121 cgcccgtggg tcacaacgag cattccgaac atgtcgactc cgtactggcg agacgccgtt
     3181 actctttggc cacgtcccgg tgactgaccg agtgggcgag cccctgcgcg gcgaggtgcc
     3241 ggcccagccg ctcggccaac gccaccgacc gatgcttgcc gccggtgcag ccgattccga
     3301 tcgtcaggta ggccttgccc tcggcgcggt acagcgggat caggtaatcg atgaagtctt
     3361 gcaaccgttc gacgaactgc cctgtcagct ccgagcgctc gagaaactcg cgcaccggcc
     3421 gatcgaggcc ggtcatgtgg cgcagctcat cgacgaagta cgggttcggc aggaaccgca
     3481 cgtcgaacaa caggtcgacc tgtgccggca ctccgtactt gaagccgaac gactcgacgt
     3541 tcacgttcgg cgtcgagatc cgctcgtcgg taccgtaggc gccggtcaag aacgagcgca
     3601 actggtgcgc ggtcagattg ctggtgtcga tcacgcggct cgacacgtcg cgcaacgggc
     3661 gcaacaccgc tcgttcggcg tggatcgcct gttcgagtgt tccgccggga gtggtcatcg
     3721 gatgcggccg gcgcgtctcg ctgaagcggc gcttgagcac atcgtccgaa caatcgaaga
     3781 acagaatctc gagcgggatg tcggcggcgc gcagaccctt gaggatctcg ggcacctgtt
     3841 cgagaaagcc gcgctcgcgc gcatcgacga ccaatgccgc caacgggatg ttctcggcgc
     3901 cacgttggat caactcgcag aacggcggga tcatctgaac cggcaggttg tcgacgcaga
     3961 agtaaccgag gtcctcgaag caattggcag ccagcgactt gccggagccg gacatgccgg
     4021 tgaccaccac caggcgatgg acggccactc aggactcccc ttcctcgggt cgccctcgcc
     4081 gcaccgccgc gcgcacttcc tcgtcttcgt cgtcgggatc gtacgcgtgg ggatggagct
     4141 tctctccgag acggcgcgcc agctcgcgtg ccgggtggta gcccttcagc ttcaacaggt
     4201 gattgcgcgc ggcgacctcg atcagcaccg acaggttgcg gcccgggccg accggcatct
     4261 cgacgtacgg cagctcgatc ccgagcactt cgtaggtctg ctcgtcgagc ccgagccgat
     4321 cgtacttctt accggcgcgc cacgggtcga gccggatcac gtactcgacg tacttgttca
     4381 tccgcaccgc ggcgacgccg aacaggtcct tgacgttgac gatgcccacg ccgcgcaatt
     4441 ccatgtggta gcgtgtcaac tcgggcccgg tgccgagcag catgttgccc ttgcgccgga
     4501 tctcgacgac gtcgtccgag accagccggt ggccgcgtac gacgaggtcg agcgcgcatt
     4561 cgctcttgcc cacgccgctg tcaccgaaca acagcacgcc caggccgtag acgtcgagca
     4621 acacaccgtg gagcacgacg cgcggggcca gccgatcttc gaggaagcgc gtcagcttgt
     4681 cgatcgtcgt cgacgacgtc aggctggtgc ggagcaacgg gatgttgtgg cgctcggtct
     4741 cctcgatcat ctcccgcatc ggctcgagat tcgaggtcag cacgaaacaa ctgccgccct
     4801 cgcggcacaa ctgcgagagg atgcgcgagc gctcgcccgg ggtccgttcg gccagaaagg
     4861 tgaactcgga cttgccgagg atctggatcc gaccggggtg gatgtactcg aggaaaccgg
     4921 cgagcgccag acccggtttc tggatccggg gttgattgac gatgttgtcg agcccctcgg
     4981 caccggatac cagcgagaga tggatccctt ccgccgcctc gtcggtcagc agttcgcgca
     5041 ccgccatcga cgggatcgcg caactctcat gctcgcccaa ggtcggctcc caacccaccc
     5101 actacagcta ttttgagatt ctaggaacag cgttaggagg tgtcaaggat ggggcagggg
     5161 gctcacgttg acacgctgtc gagggcggtg gcatagtctg ctccggtcgt accccgggtt
     5221 tcgaccaccg cattgatgcc gttgtccccc ccgccggcga ggagatgggt ccgatgagct
     5281 caagaccgat tgtgcatgtg atcggaaccg ggacgatcgg cgaaccgctg atcggtttgc
     5341 tgtcgatcat gaaagacgac ctgggcatcg aggaggtgac gttccacaag cggacgccgc
     5401 tcttgaccga tcgctccaag gtcttcgggt tgatcaacaa gggcgccaag ctggcggtcg
     5461 acgacggcgc cgcgcggggg tttcaggatc tggggatgga gcccgcgctc gagacgaagg
     5521 aggcgttgca gcgggcggcc gtcgtgatcg actgcacgcc cagcggggtc ggcacgcaga
     5581 acaagaccga gttctacgag aattacacgt cgaacaccct cggcttcatc gcgcaaggca
     5641 gcgagttcgg cttcggcaag ccttatgccc gcggaatcaa cgatcgggca ctcgtccgcg
     5701 gcaaggacca gttcatccag gtcgtgtcgt gcaacacgca caacctgtcg atcctgatcg
     5761 acacgctggc gctggaggac ggtggaccgc ccaacctgaa cgagggccgt ttcgtctgca
     5821 tgagacgggc gaacgacatc tcgcaggaca gcagcttcgt cccgtcaccg caggtcggca
     5881 ggcacaagga cgctcgtttc ggcacgcacc acgcgcgcga cgcgtggcac ctgttccaga
     5941 ccctgggcta cgacctgaac ctgttctcgt cggcgctgaa gctcaacacg cagtacatgc
     6001 acaccatttt cttcgacatt cgcgtgaaca acccgacgac ggtcgacagc ctgcaagagc
     6061 ggatccacgc caatgaccgg atcgcggtaa cctacaagac gtcggccaac agcgtcttct
     6121 cgttcggtcg cgatcagggc cactacggcc gcatcctcaa cgtttgcgtg gtcgcgctac
     6181 cgacgctgat ggtgcgcaac gagaaagaga tcctgggata ctgcttcacg ccgcaggacg
     6241 gcaattcgct gctgtcgtcg atctcggcgg cgacctggtt cctctacccc gacgactacg
     6301 aggatcggat ccagtgcgtg gcggagtact tccacgacga agtgtagcgg gacctcagtt
     6361 cttgagctcg cggaccgccc gcgtcagcgc ggggaggacg tcgaacaggt cgccgacgat
     6421 gccatagtcc gcgaccttga agatcggcgc gtcggggtct ttgttgatcg cgacgatgac
     6481 ggccgaggac gacatcccgg ccaggtgctg gatcgcaccc gatatgccgg ccgcgatgta
     6541 cagagtgggc gaaacgacct tgccagtttg accgacttgg tgcgagtgtg cgatccaccc
     6601 ggcgtcgacc acggcacgcg aggcgccgac cgcgccgccg agcgcatcgg ccagctcgcg
     6661 caccagcgag aagttcttcg gatccttgag accccgtcct cccgaaacga tgatcgaggc
     6721 ctcggccacg tcgagctctt ggcggtcggg cttgcgcagc tcgcgcgtgg tcacgcgcgc
     6781 cgccgcggga tcgagcggca ggccgagggc ctcggcggcg cccgccgccg gggccgccgc
     6841 ctcgctcgcg gcgaatacgt tggggcgcag cgtggcgacc gccggtcgtg ccttgagcac
     6901 gacccgggcg accgccttcc cggagtaaac cggtcgcttg gcgcgtagcc cgcccgatcg
     6961 atcgacctcg atctcgacca cgtcgctggc gcacgccgtg tcgaggcgcg ccgcggtccg
     7021 tgcggcgagg tccttgccca tcgcggtcgc cgccaacagc acgaagtcgg cctccgcccg
     7081 gccggtcgcc tcgaccagac aggccgagta gagctctgga gaatacagct cgaccgacgg
     7141 atcctcgacg tggaacacgc gttcggcacc gtaacgcgcc aggctcgccg ccgcggccgc
     7201 cgcacccgga cccaacacca acgcgtcgac gcggccggac cccgcggcgg ccagacgccg
     7261 gccctcggtc aggacctgca acgacggccg cttgatctcg ccctcccggc gttcgacgaa
     7321 caccaacacg ttggccatcc tcagatcacc ttcgcttctt gatggagcaa acgcgccagc
     7381 tcccgggctg cctgctcggc gtctccgtcg attagacgac cggacgcgcg ttcgggtgga
     7441 agctcgagcg cttcccaggc cagctttttc tctccttcga gctccgcgtc gccgacgccg
     7501 cattgctcgg cggtccgcag gtcgagcggc ttcttcttcg cctgcatgat ccccttgagt
     7561 gacgggtagc gcgggtcgtt cagccccttc tcgcagctga tcaccgccgg cagactgccg
     7621 tcgacgacct cgaccgcccc ttcgacctcg tgctcggcgg tgaaccggcc atcgacgagc
     7681 tcgagcttgg agacggcgaa ggtgttgggc cacgcgagca gctcggccag catcgcgccg
     7741 gtctgcccct catcggtgcc gaccccgtac ttgccgacca gcaccaggcc cgccccctcc
     7801 tcacgaacga cggcggcgag cgccttggcc cgggtcagac cgtccgccag atcgaagcgc
     7861 ggatcctgga tccacaccgc gcggtcggca cccgtcgcga gcgcctgacg cgccacctgt
     7921 tgggcgctgt cccggccggc ggagaccgcc accacctcgg acgccacgcc gcgctccttg
     7981 agctgcaacg cctgctcgag cgcgaactcg tcgtacggcg agatgatcca cttggtgacc
     8041 gccgcaggat cgatcgcgaa gccgtcggga ccgaccttga tcttggtctc cgtgtcggga
     8101 accggcttga ttccgactac gattctcatc gtgactccgt tccccggtcc gcatcggcgg
     8161 tcaggcttcg tgcttggcca gcagcaccgt cgcgttggtg ccgccgaagc cgaacgagtt
     8221 gttcagcgcc gtcctgaccg tcatcgagcg tgcctcgttc ggcgcgcagt cgagatcgca
     8281 ctcgggatcg gggaattcct ggttgatcgt cggtggaacc cgatcgtgga agacggccag
     8341 cgcggcgacc gcggtctcga gcccgccggc agcgccgagc aagtgacccg tcatcgactt
     8401 ggtcgagccg gcgacgagct ggtaggcgtg cttgccgaac agcttcttga tcgccatcac
     8461 ttcgatccgg tcgcccgcgg gcgtcgacgt tccgtgcgtg ttgatgtagt ccacatcgcc
     8521 cggagcgaca ccggcgcggt cgagcgccat gcgcatcgcc cgtaccggcc catccccatc
     8581 gatcgacggt gccgacacgt gatacgcatc gccgctcacg ccgtagccgg tgatctcggc
     8641 gtagatcctc gcgccgcggg ccagcgccgc gtgacgttcc tccagcagca ggatacccgc
     8701 gccctcgccg atcacgaagc cgtcccgctg ggcgtcgaac gggcgactgg cccgctccgg
     8761 ctcgtcgttg cgcgtcgata gcgccttcat cgcgcagaac ccaccgacgg cgagcgacgt
     8821 gatcaccgcc tccgtcccgc cggcgatcat cgtttcggcg tagtcctcgc ggatcatccg
     8881 atacgactcg ccgatggcgt ggctgcccgt ggcacacgcg gtggctagcg ccaggttcgg
     8941 ccctttggcg ccgaacagga tcgacacgtg accggccgcc aggttgagga tcatccccgg
     9001 gatgaagaac ggcgaagtcc ggcgtggacc tcgctcgagt atctgtcgat aggtcgattc
     9061 gagcagcggc aaaccaccga tcccggagcc gatgtaaacc ccgactcgct cggcgttgcc
     9121 gtcgtcgatc gtgaaaccgg catcgtcgag cgcttgcttg gccgcggcga cggcgtagtg
     9181 cgagaaggag tccattttac gggcctcctt cggttcgatg tatgccgtcg gatcgaagcc
     9241 cttgacctcg cccgcgatct gtgacgagag attcgacgcg tcgaacttgg tgatgcgatc
     9301 gattccgctc ttgccggcga gcagcgcttg ccatgagctc gttgtgtcga gccccacggg
     9361 ggtgaccaaa ccgatgccgg ttatgacgac tcgcctagtc acggattctc tccggcgtga
     9421 agacgatcgc gtcccccttg tcggtcgggc gacgagcggg tggatcgact ctgcctcagt
     9481 ccttggcgtt ggctccgatg tagtcgaccg cctgcttgac ggtccgtagc ttctcggcgt
     9541 cctcgtcgct gatctcgagc ccgaattcct cctcgagcgc catcaccaac tcgacgacgt
     9601 cgagcgagtc ggcccccaag tcgtcgacga aggacgcctc cggtttgacc tcgtcctcgt
     9661 ccactccgag ctgttcgacg atgatgtgct ttactttttc ctcgaccgaa gccatcgcgt
     9721 ctcctccgtc gcgttcaccg ggttccacgg atcattcccc ggctccccgc gttcgtctac
     9781 atgtacattc cgccgttcac gtgaatcgtc gtacctgtga tgtaatccgc ccccccaccg
     9841 accaagaatc gcacgcagtg ggcgacgtcg tccaccgtgc cgagccggcc cagcggaatg
     9901 cggtcgagca acgcctgtcg ttgcgtgtcg tcgagcgcgc gggtcatgtc ggtatcgatg
     9961 aaaccgggcg caacgcagtt gaccgtcacg ttc
//
LOCUS       DASPGN010000142        10441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_23474, whole genome shotgun sequence.
ACCESSION   DASPGN010000142 DASPGN010000000
VERSION     DASPGN010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23474"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VD788_03920"
     CDS             <1..621
                     /locus_tag="VD788_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV75442.1"
                     /translation="LRKKFVGSGEELEGVVAEATESIEREVHALKQLVDEFSLFARLP
                     EIRPRRVDLAPLIDSVVALYTGLPDVDWNVRVGAGLDGVRIDPDQMRRVLINLVDNAV
                     TAMGRRGQIRIAADRLEDGSTVRIEVADTGPGIDPADRDKMFNPYFSTTKRGTGLGLA
                     IVHKVVSDHDGTIRVEPNDPHGARFVIDLPGIEPVPERRVGGAGGA"
     gene            611..1906
                     /locus_tag="VD788_03925"
     CDS             611..1906
                     /locus_tag="VD788_03925"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV75443.1"
                     /translation="MARERVLVVDDEPGVRSALEPILADEGFEVTTVSSGEAALDALA
                     DREFDAVLLDVWLPGIDGLETLRRLRERNDDVEVVMISGHGTIDTAVKATKFGAFDFI
                     EKPLSLERTLLVMRNALRQRRLRQSNRQLLEQLGRDTEIVGRSERTEALRGLVEIAAG
                     SDAPVLICGEQGSGRENVARRIHARGRRRAAAFVEVPCGALDAERAASALGLDASDGG
                     RVALARNGTLFLEDVDRLAPELQRRAAATLLTRTREGTDIRVLASAPTDAAGVHDELT
                     GLVDVIRIDVPRLRDRREDIAMLVERFMHDLTREYGKGPKRLTAESLAALRANDWPGN
                     VRQLFNLIERLVLVVPGERVTVDDLPLEFGGAGHPVEDLYGDFESLERGLRVFEGYQL
                     RRAIARSSGDVEQAAVALGLERKELERRLRRMARDPEGS"
     gene            complement(1903..2511)
                     /locus_tag="VD788_03930"
     CDS             complement(1903..2511)
                     /locus_tag="VD788_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV75444.1"
                     /translation="MMSDCEQIRPLLFPYLEREVEPAEAIRVANHLSACTACKILMAR
                     KRRLGQMLEDGLEDSIPVGEDFVRSVMATLPDGPPPGPQQERKRRRRFDLALVIGAAV
                     GVGVTQALAAGWPPLSIEPGGWAWPALDLPVVGDPGAGLSSLFRSAAIASDGLASLAA
                     IHSASFSTLGATVVAGAFGVVAFALLGGSTVLAVAWRRQGNP"
     gene            complement(2511..3068)
                     /locus_tag="VD788_03935"
     CDS             complement(2511..3068)
                     /locus_tag="VD788_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV75445.1"
                     /translation="MGTAAAGDRGAFERLVRRHQRALVNHLYRHTGQRDLAVDLAQEV
                     FLKVYLSLTSFDPRYRFTTWLYRIASNCAIDHLRKKQPRTCSLHADAQDEHADAPART
                     LAGTEPTPHEVLRALEIQRRLDVAVRRLPPDYRQLILLRHRQHCRYDEIARITRLPIG
                     TVKNRIFRAREMLRVDLADVLDLET"
     gene            3377..4594
                     /locus_tag="VD788_03940"
     CDS             3377..4594
                     /locus_tag="VD788_03940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXV75446.1"
                     /translation="MRRTNPEGEGSLGEPGEAVSVADRRRLAALLADPRLDGARLLAR
                     LREARTLDRIASCSSIIETLCGIRLAEAAAERLIVELVEHREQWIRRVGRDPGLAVVA
                     MDRLVEGADGARNPVAVELSELDPGGTTVVPDPLTGLNHRRFLEAALWREIRRSGRSR
                     IGFSVVVADLDGFRSVNELYGHPLGDRVLGRAGRTVRRTVRESDTAWRLDADQFAVLL
                     PEGRRLGAWGAAERLRAAVEADFLSTTIDDRIVVMTVSCGVACYPDDGTTPDDLLAAA
                     RCAVSRAKMRGANRVMIHPRERRRSVRLAARPTARARLGAGSIRELHDVRLIDLGRNG
                     ALVETDADVPGDEPLLLVLESVGRSTMVHCRLTRTESDSTARRLAVLFAGQLERRTVA
                     EFGMAAAPADGGR"
     gene            4591..5838
                     /locus_tag="VD788_03945"
     CDS             4591..5838
                     /locus_tag="VD788_03945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXV75447.1"
                     /translation="MITREEIERVRDDLIRLVDEDAYNTRRLLDRLESIAQESGVGAH
                     AALITILTRLVFDEAEARRHWTKIIEHREWLTSALGREVAVRVATLDYFVHVNRHLLQ
                     PTLIDLDMVDAHAADASIDRLTGLSNDRVFRVGLQTELRRARRYRLPAAVLLFDVDDF
                     AAANHRFGRLVADRLLREAAILLKNKTRDIDVAARPGEDEIAMLLPETDRNGALLVAE
                     RCRVEFERYFAGREAGGRPAALTVSGGVAAFPEDATSPEELLERAAQALYQAKATGRN
                     AVQSYRPERRHYLRFEFEAGRFEVEVLAPRDVAGASLCNVSRNGILFLSPEPIDVGES
                     IEIRLLDAHDASGGGRAIRGTVVRLEQLPSPAEVDLPGEDAARSRDRFEIGVALALGA
                     DDGPVQLSAFLESLQTGRLARRG"
     gene            5841..6533
                     /gene="tsaB"
                     /locus_tag="VD788_03950"
     CDS             5841..6533
                     /gene="tsaB"
                     /locus_tag="VD788_03950"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXV75448.1"
                     /translation="MRIVAVDASSWWGGVALLERRDGRSAEAAELGFEVRSSHVGSLL
                     AAIDHALALADWNRSSLDGFVATRGPGSFTGVRIGLGIVRGLGLAADRPCAGVATLDS
                     LAAAFGPAEGERLALISAGRGEIFGARYHAVGVGAPLDGPWLMPQRRLADALADGAVT
                     VVIPAKGSEPAVRGMELERFGIRVAAAPGAVATAAGRLALSAGLLDDPDDATMAPLYL
                     RPPDAELESFSS"
     gene            6556..7044
                     /gene="rimI"
                     /locus_tag="VD788_03955"
     CDS             6556..7044
                     /gene="rimI"
                     /locus_tag="VD788_03955"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HXV75449.1"
                     /translation="MRLRRMEVSHLDQVLRIERASFGSPWKMEHFLHEIEGNRWAVNW
                     VMEDPAARVIGYSCCWCIHDELKINNVAIHRDHRNRGFGRWLLLNVLREALLRGCKSA
                     TLEVRPSNVSAIRLYQAFGFREIGRRSGYYAAEGEDAIVMHAELDRRRWRAIASAARR
                     AV"
     gene            7172..7390
                     /locus_tag="VD788_03960"
     CDS             7172..7390
                     /locus_tag="VD788_03960"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HXV75450.1"
                     /translation="MHSTGDLRQRLVRDDPDYQRLSRKHDEYDRRLAELRAQKYLSQD
                     EQMEEVRLKKLKLAIKDRMEAIVRRAMA"
     gene            7417..8037
                     /locus_tag="VD788_03965"
     CDS             7417..8037
                     /locus_tag="VD788_03965"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HXV75451.1"
                     /translation="MTIDRAGLPFASFAALATAAAVWWVGPWFAVAPGIATLFVVWFF
                     RDPRRVIPSGDGLLVSPADGKVLVASRDKISVFMNVFDVHVCRTPAAGRVTTVDHVAG
                     RFLAAWRDDAGEHNERVTIVVNGADTELRFVLIAGLIARRIVCRVHVGQRLERGDRVG
                     LIRFGSRVEVLLPPGCEPRVRRGDRVVAGETVLAGVVPGSGRAEAR"
     gene            8098..8862
                     /gene="pssA"
                     /locus_tag="VD788_03970"
     CDS             8098..8862
                     /gene="pssA"
                     /locus_tag="VD788_03970"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HXV75452.1"
                     /translation="MGRNGKFRRGIYLLPTLFTIGNLFCGFYAVVSASRGEFERAALM
                     VIVAGVLDGLDGRIARMTRSTSAFGTQFDSLADLVSFGIAPAMLAYHWALAPLGRIGW
                     LVPFLYLVCAAMRLARFNIKVASSDKRFFAGLPSPGSAGALASLVFAFPAPPGGTGLV
                     VAVAVLVGTLGLLMLSTFRYRSFKDLDLAHRRSYLVILPLAAMLVAVAIQPKGALLLF
                     ASVYLMSAPLGYLWGLLSAQPRRTRVEEVADGRVAR"
     gene            8843..9847
                     /locus_tag="VD788_03975"
     CDS             8843..9847
                     /locus_tag="VD788_03975"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HXV75453.1"
                     /translation="MGASLAERTSGARVALVGSGSSYGHVVRQALVDFGVPGSRVDLY
                     GVAVGDEPVISEYAGEARLIQQPEVGELTSRDVVFLCEAGGVVDDLFGSSNCAHVSIV
                     DVVHARPAGRPARLVHMDVNPAAATAGDTRVLAVPHDLSTLLVDLLHPLDAEFGCEEA
                     IAFVLRPASDAGQSGVDELRRQTVGLLNFASTPTEVFGRQLAFNIVPQSEVGESGVES
                     RIAEEVATLLGWDRRRLSLSLGFAPVFFGHTVQLRVRTERSSSAEALRAVFALAGLVP
                     DADDPRTTPMDVSTEKRRCISRVADDGSGGYWLWAVAGEARVRNAELAVRLADAVCAL
                     "
     gene            9878..>10441
                     /locus_tag="VD788_03980"
     CDS             9878..>10441
                     /locus_tag="VD788_03980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75454.1"
                     /translation="MKLRCGCLFLAIGLLWTAAVPAGDDTLAFADGGSSGGGAVPAIR
                     AGIDVEKTLLEESLARYRADASRRTALLGRIVELYAELDRVVGAPEEDAPRSVETLLD
                     QIATAEGERSRLLLSEHALVRAIGTHVRRIALLEEQLVVLEGQREQAAGALAGEWDVV
                     LLPLEQRGTFRLMQSGTLISGTYELAGG"
BASE COUNT         1508 a         3384 c         3859 g         1690 t
ORIGIN      
        1 ctgcgcaaga agttcgtcgg cagcggcgag gagctcgagg gcgtcgtggc cgaggcgacc
       61 gagtcgatcg aacgggaggt gcatgctctc aagcagctgg tcgacgagtt ctcgcttttc
      121 gctcgtcttc ccgagattcg cccgcgcagg gtcgacctcg cgccgctgat cgactcggtc
      181 gtcgccctgt ataccgggct gcccgacgtc gattggaacg tccgggtcgg cgccgggctg
      241 gacggagtgc ggatcgaccc ggaccagatg cggcgcgtgc tgatcaatct cgtcgacaat
      301 gcggtcaccg caatgggtcg gcgcggtcag atccggatcg cggccgaccg gctggaagac
      361 ggctcgacgg tacggatcga ggtggcggac accggtccgg gaatcgaccc ggcggatcgg
      421 gacaagatgt tcaatcccta tttctcgacg accaagcgcg gaaccggatt ggggctggcc
      481 atcgtgcaca aggttgtgag cgaccacgac gggacgattc gcgtcgagcc gaacgatccg
      541 cacggtgccc gtttcgtcat cgacctaccg ggcatcgagc cggtgccgga gcggcgggtg
      601 ggaggggcag gtggcgcgtg aacgcgttct ggtcgtcgac gacgagccgg gcgtgcgctc
      661 tgcgctcgag ccgatcctcg ccgacgaagg cttcgaggtg acgaccgtct cgagcggcga
      721 agcggcgctc gacgcgctcg ccgatcgcga gttcgacgcg gtcctgctcg acgtgtggtt
      781 gcctgggatc gacgggctcg aaaccctgcg caggctgcgc gagcgcaacg acgacgtcga
      841 ggtggtgatg atctccgggc acgggacgat cgatactgcg gtcaaggcga cgaagttcgg
      901 tgcattcgat ttcatcgaga agccgctctc gctcgagcgc acgttgctcg tcatgcgcaa
      961 cgcgctgcgg cagcgccggc tgcgccagag caatcgccaa ctgctcgagc agctcggtcg
     1021 cgacaccgag atcgtcggcc gcagcgagcg cacggaggcg ctgcgcggcc tggtcgagat
     1081 cgccgccggc tccgatgcac cggtgctgat ctgcggagag cagggctcgg gtcgagaaaa
     1141 cgtcgcgcgg aggatccacg ctcgagggcg gcggcgcgcg gcggcgttcg tcgaggtccc
     1201 gtgtggggcg ttggacgccg agcgggccgc gagcgcgctc gggctcgacg cgtcggacgg
     1261 cgggcgggtg gcgctcgccc gcaacggcac gctgttcctg gaggacgtcg accgactggc
     1321 gcccgagctg cagcgtcggg cggcggcgac cctgctcacg cggacgcggg aggggaccga
     1381 catccgcgtg ttggcctcgg cgccgacgga tgccgccggc gtccacgacg agctcaccgg
     1441 gctggttgac gtgattcgta tcgacgtgcc tcgcctacgg gatcgtcgcg aggacatcgc
     1501 gatgctcgtc gagcggttca tgcacgacct cacgcgcgag tacggcaagg gaccgaaacg
     1561 gctgaccgcg gaatcgctgg cggcgttgcg cgccaacgat tggcccggca acgtgcggca
     1621 gctgttcaat ctgatcgagc gcttggtgct ggtcgtaccg ggggagcggg tcaccgtcga
     1681 cgatctaccg ctcgagttcg gcggtgccgg gcacccggtc gaggatctgt acggcgattt
     1741 cgaatcgctc gagcgcggtc tgcgggtgtt cgagggctat caactgcgcc gggcgatcgc
     1801 gcgcagctca ggcgacgtcg aacaggcggc ggtagcgctc ggtctcgagc gaaaagagct
     1861 ggaacggcgt ctccgccgca tggcccggga cccggaggga tcctaagggt tgccctgacg
     1921 gcgccaggcg acggccagca ccgtcgagcc tcccagcagc gcgaaggcga cgacgccgaa
     1981 cgcaccggcg acgaccgttg caccgagtgt ggaaaacgaa gccgaatgga tcgcggccag
     2041 cgaggccagg ccgtcggacg cgatcgccgc gctgcggaac agactcgaga ggcccgcgcc
     2101 gggatcgccg acaacgggaa ggtcgagcgc cggccaggcc cagccacctg gctcgatcga
     2161 cagcgggggc cacccggcgg ccagcgcctg ggtcactccg actccgaccg cggctccgat
     2221 gacgagcgcg agatcgaagc gccggcgtcg cttgcgttcc tgttgcgggc cgggcggggg
     2281 accgtcgggc agcgtcgcca tcaccgaccg gacgaaatcc tcgccgaccg ggatactgtc
     2341 ctccaggccg tcttccagca tctgacccag gcggcgtttg cgcgccatca ggatcttgca
     2401 ggccgtacag gcggagaggt ggttggcgac gcggattgcc tcggccggtt cgacctcccg
     2461 ctccaagtag gggaagagca acggccggat ctgttcgcag tcggacatca tcaggtctcc
     2521 aagtccagca cgtcggccaa gtcgacccgc agcatctctc gtgcgcggaa gatccggttc
     2581 ttgaccgtgc cgatcggcag cctcgtgatg cgggcgatct catcatagcg gcaatgctgt
     2641 cggtggcgca gcaggatcaa ctggcggtag tcgggtggca gccgtcgcac ggcgacgtcc
     2701 aagcgtcgtt gtatctcgag cgccctgagg acttcgtgcg gcgtcggctc ggttccggcc
     2761 agcgttcgcg ccggagcatc cgcgtgctcg tcctgggcgt cggcatggag cgaacaggtg
     2821 cgtggctgtt tcttgcgcag gtggtcgatc gcacagttcg aggcgatgcg gtacagccag
     2881 gtcgtgaacc ggtagcgggg gtcgaagctc gtcagcgaca ggtacacctt gaggaacacc
     2941 tcttgcgcca gatcgaccgc caagtcgcgc tggccggtgt gacggtaaag atgattgacc
     3001 agtgcccgct ggtggcgtcg caccaagcgc tcgaaggcgc cacggtcacc cgcggccgcg
     3061 gttcccacga gttgttcatc gctctcggcc ggctttgacg ccggccgccc ccgtttcgga
     3121 tgcaatccgc gttcggccaa gtaccccccc cctctcgccg ggcagtgaac cgaaccaccc
     3181 ccctttagta cgccgccgag ggtcgagagt ttcaaccgat tccggcgact ccgtcgaccc
     3241 cacccgtcgc ggcgaccgaa ttgacaccgg cggttggacg gggctacgat gacgcggagt
     3301 ggcgaatccc tcaggccggc gatgaaccga cccccccgag gggaagccgg aatgcccgcc
     3361 atctcgaggt gggacggtgc ggagaacaaa cccggaaggc gagggttccc tcggggagcc
     3421 gggcgaggcg gtgtcggtcg ccgaccggcg ccgcttggcc gctcttctcg ccgatccgcg
     3481 gttggacgga gcccgcttgc tggcgcggct ccgcgaagcc cgcaccctgg acaggatcgc
     3541 gagctgctcg agcatcatcg agaccttgtg cgggatccgg ctggcggagg ccgcggccga
     3601 gcgcttgatc gtcgagctgg tcgaacaccg cgagcagtgg atccggcggg tcggccgcga
     3661 tccgggtctg gcggtggtcg cgatggaccg gctggtggaa ggggcggacg gggcgcgaaa
     3721 cccggtggcg gtcgagctgt ccgagctgga tcccggcggg acgaccgtcg tgcccgaccc
     3781 gctgaccgga ctgaaccacc gccgttttct cgaggccgcg ttgtggcggg aaatacgtcg
     3841 ctccggacgg agccggattg gcttctccgt ggtcgtcgcg gacctcgacg ggttccgatc
     3901 ggtcaacgag ctgtacggcc atccgctcgg cgaccgggtt ctcggccgcg cgggtagaac
     3961 ggtccgccgc accgtgcgcg agtcggacac ggcctggcgt ctcgacgccg atcagttcgc
     4021 cgtgctgttg ccggaaggac ggcgcctcgg cgcgtggggg gcggcggagc ggcttcgcgc
     4081 cgcggtcgaa gccgatttcc tctccacgac gatcgacgat cggatcgtgg tgatgaccgt
     4141 ctcctgcgga gtcgcctgct acccggacga cggtacgacg cccgacgatc tgctcgcggc
     4201 ggcccgctgt gcggtgtcgc gcgccaagat gcggggcgcc aaccgcgtga tgatccaccc
     4261 gcgcgagcgc cgtcgctcgg ttcgtctcgc cgcgcggccg acggcgcgag cccggttggg
     4321 cgccgggtcg atccgcgagc tgcacgacgt acggttgatc gacctcggcc gaaacggcgc
     4381 gctcgtcgag acggacgcgg acgtccccgg cgacgagccg ttgctgctcg tgctcgaatc
     4441 cgtcggccgt agcacgatgg tgcactgccg gttgacgcga accgagtcgg actcgacagc
     4501 gcggcgcctg gccgtccttt tcgcgggcca gctcgagcgg cggacggtgg ccgagttcgg
     4561 catggcggcc gcgcccgcgg acggcggccg gtgatcactc gtgaggagat cgagcgggtc
     4621 cgagacgacc tgatccgcct ggtcgacgag gacgcctaca acacccgtcg cctgctggac
     4681 aggctcgagt cgatcgcgca ggagagcggc gtcggtgcac acgcggcgtt gatcacgatc
     4741 ctgacgcgcc tcgttttcga cgaagcggag gcgcgccggc actggaccaa gatcatcgag
     4801 catcgcgagt ggctgacgag cgcactcggt cgcgaggtcg ccgtacgcgt cgcgacactc
     4861 gactacttcg tccacgtcaa tcgccacctg ctgcagccga cgttgatcga tctcgacatg
     4921 gtcgacgcac acgcggcgga cgcgtcgatc gatcgactga ccggcctgtc caacgatcgt
     4981 gtttttcgcg tcggcctgca gaccgagctc cgtcgggcgc gtcgctaccg gcttccggcc
     5041 gcggtgctgc tgttcgacgt cgacgacttc gcggcggcga accaccggtt cgggcgactg
     5101 gttgcggacc gattgctgcg agaggccgcg atcctgctca agaacaagac ccgggacatc
     5161 gacgtcgcgg cccgacccgg agaagacgag atcgcgatgt tgttgcccga aacggaccgg
     5221 aacggggcgt tgctcgtcgc cgagcggtgt cgcgtggagt tcgagcgcta cttcgccggc
     5281 cgggaggccg gcggtcgtcc ggcggcgctc accgtgtcgg gcggtgtggc cgcgttcccg
     5341 gaggatgcaa cctccccgga agagctgctc gagcgggccg cgcaggcgct gtatcaggcc
     5401 aaggcgaccg gacgcaacgc ggtgcagtcc taccggccgg agcgccgcca ctacctccga
     5461 ttcgagttcg aggcgggtcg tttcgaggtc gaggtgctgg caccgcgcga cgtagccggg
     5521 gccagtctgt gcaacgtgag ccggaacggc atcctgttcc tcagcccgga gccgatcgac
     5581 gtcggcgagt cgatcgagat tcgtctgctc gacgcgcacg acgcgagcgg cggtgggcga
     5641 gcgatacgcg gcacggtcgt acggttggag cagctaccga gcccggccga ggtcgatctg
     5701 cccggggaag acgcggcgcg ctcccgcgac cgattcgaga tcggcgtggc gctcgcgctc
     5761 ggagcggatg acggcccggt gcaactgtcg gcgtttctcg agtcgctgca gacggggcgg
     5821 ctcgcgcgcc gcggctgacc gtgcgcatcg tcgcggtcga tgcgtcgagc tggtggggcg
     5881 gagtggccct gctcgaacgg cgggacgggc ggtccgccga ggccgccgag ctggggttcg
     5941 aggtgcgatc gtcgcacgtc gggtcgttgc tcgcggcgat cgaccacgcg ttggcgctcg
     6001 ccgactggaa ccgctcctcg ctcgacggct tcgtcgcgac gcgcggtccc gggtcgttca
     6061 ccggcgttcg gatcggactc ggcatcgtcc gcggactggg cctggccgcc gatcgcccgt
     6121 gcgccggcgt ggcgacgctt gactcgctag cggcggcgtt cggtccggcc gagggggaac
     6181 ggctggcctt gatctcggcc gggcgaggcg aaatatttgg agcgaggtac catgccgtcg
     6241 gggtcggcgc tccgctcgac gggccgtggc tgatgccgca gcggcggctg gccgacgcgc
     6301 tcgccgacgg cgccgtcacc gtggtgattc cggcgaaggg gtccgagccg gcggtgcggg
     6361 gcatggaact cgagcggttc gggatccggg tggccgcggc tcccggtgcg gtggcgacgg
     6421 cagccggtcg gctggcgctg tccgccgggc tactcgacga tcccgatgac gcgacgatgg
     6481 ctccgctcta tttgaggccg cccgatgctg aactcgagtc gttctcttcg tgaagtggcg
     6541 ccacccgagg cgggggtgcg gctgcgtagg atggaagtgt cgcacctcga ccaagtgctg
     6601 cgcatcgagc gcgcgtcctt cggctcaccg tggaaaatgg aacacttcct acacgagatc
     6661 gagggcaacc gttgggcggt caactgggtc atggaggatc cggcagcgcg ggtcatcggc
     6721 tactcgtgct gctggtgcat ccacgacgag ctgaagatca acaacgtcgc gatccaccgc
     6781 gatcaccgga atcgggggtt cggccgctgg ctgctgctca acgtgctgcg cgaggcgctg
     6841 ttgcgaggct gcaagtcggc gacactcgaa gtgagaccgt cgaacgtctc ggcgatccgc
     6901 ctgtaccagg cgttcggctt tcgggagatc ggccgccgga gcgggtatta cgccgccgag
     6961 ggcgaggacg cgatcgtgat gcacgccgaa ctcgatcggc ggcgatggcg ggcgattgcc
     7021 tcggccgccc gacgcgcggt atagttgctg ccggaattga gaccgccaac ccatcgtcgt
     7081 tcgaaacccg agaaaccgcg atccggtgtg cgtggccccg caacgcccgc atcgacgtgt
     7141 gcgccgcgcg gatgcgggac gggaggagcg gatgcacagc accggtgacc tgcgccaacg
     7201 cctggttcga gacgacccgg actatcagcg gttgtcccgc aagcacgacg agtacgaccg
     7261 ccggctggcc gagcttcgag cgcagaagta tttgtcgcag gacgaacaga tggaagaggt
     7321 tcgtctcaag aagctcaagc tcgcgatcaa ggaccggatg gaagccatcg ttcgacgcgc
     7381 gatggcctga gccgctcacg gagtggttgc ctcgcgatga cgatcgatcg ggcgggcctg
     7441 ccgttcgctt cgttcgcggc gctggcgacg gcggccgcgg tctggtgggt cggcccgtgg
     7501 ttcgcggtcg caccgggaat cgcgaccctg ttcgtcgtct ggttctttcg cgacccccgg
     7561 cgcgtcatcc cgtcgggcga cgggctgctc gtcagccctg ccgacggcaa ggtcctggtc
     7621 gccagccggg acaagatctc ggtgttcatg aacgtcttcg acgtgcacgt ttgtcgcacg
     7681 ccggcggcgg gacgggtcac cacggtcgac cacgtggcgg gacgtttcct cgcagcctgg
     7741 cgtgacgacg ccggtgaaca caacgagcgc gtcacgatcg tcgtcaatgg cgcggacacc
     7801 gagctccgtt tcgtgttgat cgcggggttg atcgcgcgac gaatcgtctg ccgagtacac
     7861 gtcgggcagc ggctcgagcg cggcgaccgc gtcggcttga tccgattcgg gtcgcgtgtc
     7921 gaggtcctgc tgccgcccgg ctgcgagccg agggtccggc gcggcgaccg cgtggtggcc
     7981 ggcgagacgg tgctggcggg cgtggtcccc ggcagtggcc gcgcggaggc tcgctgagcc
     8041 gtttttggct atagtggaac gacccggcca agccgctgcg cggccgacgg gggtgtgatg
     8101 ggacgaaacg ggaagttcag gcgcgggatc tacttgttgc cgacgctgtt caccatcggc
     8161 aacctgttct gtgggttcta cgcggtggtg tcggcctcgc ggggcgagtt cgagcgggcg
     8221 gcgctgatgg tcatcgtcgc cggcgtgctc gacgggctcg acggtcgcat cgcgcggatg
     8281 acgcggtcga cgtcggcgtt cggcacgcag ttcgattcgc tggcggatct ggtctcgttc
     8341 ggcatcgcac cggcgatgct ggcctaccac tgggccctgg caccgctcgg ccgcatcggc
     8401 tggctggtgc cgtttctcta cttggtttgc gccgcaatgc gtctggcgcg gttcaacatc
     8461 aaggtcgcca gcagcgacaa gcggttcttc gccggcctgc cgtcaccggg atccgccggc
     8521 gcactggcca gtctcgtttt cgcgtttccg gcaccgccgg gaggcaccgg attggtcgtc
     8581 gccgtcgccg tgctggtcgg cacgctcggc ttgctgatgc tcagcacgtt tcgctatcgc
     8641 tcgttcaagg atctcgatct ggcccatcga cgttcctacc tcgtcatcct gccgttggcc
     8701 gcgatgctgg tcgcggtcgc gatccagccc aagggcgcac tgctgctgtt cgcctcggtc
     8761 tacttgatgt cggcaccgct ggggtacctc tggggcttgc tgagcgccca gccgcggcgc
     8821 acgcgcgtcg aggaggtcgc ggatgggcgc gtcgctcgct gaacgtacct ccggggcgcg
     8881 cgtggcgctg gtcgggtcgg ggtccagtta cggacacgtc gtgcggcagg cgctcgtcga
     8941 tttcggcgta ccgggttcgc gggtggatct gtacggggtg gccgtcggcg acgaaccggt
     9001 gatcagcgag tacgcgggtg aggcgcggtt gatccagcag ccggaggtcg gcgagctcac
     9061 ctcgcgcgac gtggtcttcc tctgtgaagc cggcggggtc gtcgacgacc tgttcggcag
     9121 cagcaattgc gcccacgtct cgatcgtcga cgtggttcac gcgaggccgg cggggcgtcc
     9181 ggcgaggctg gtccacatgg acgtcaaccc ggcggcggcg acggcgggcg acacgcgcgt
     9241 gctggccgtc ccgcacgatc tctcgacgct gctcgtcgat ctgctccacc cgctcgacgc
     9301 ggagttcggc tgcgaggagg cgatcgcctt cgtcctgcgg ccggcctccg acgccggtca
     9361 gtcgggtgtc gacgagctgc gccggcagac cgtcgggttg ctcaacttcg cctcgacgcc
     9421 gaccgaggtc ttcggccggc agctcgcctt caacatcgtg ccccagtccg aggtcggcga
     9481 atcgggagtc gaatcgcgga tcgccgagga agtggcgacg ttgctcggct gggaccggcg
     9541 ccggttgtcg ctgtccctcg gttttgcacc ggtgttcttc ggacacacgg tgcagctgcg
     9601 cgtgcgcacc gaacgatcga gctcggccga ggcgctgcga gcggtcttcg cactcgccgg
     9661 cctcgtcccc gatgcggacg atccgcggac gacgccgatg gacgtctcga ccgagaagcg
     9721 acgttgcatc tcgcgcgtcg ccgacgacgg cagcggaggg tactggctgt gggcggtggc
     9781 gggcgaggcg cgcgtccgca atgccgagct ggcagtacgg ttggcggacg cggtctgcgc
     9841 actctgaccg gcgcggcacg gcgacggggg tgaaccgatg aagctccgat gcggctgcct
     9901 gtttctcgcg atcgggctgc tgtggacggc ggccgtgccg gccggcgacg acacgctggc
     9961 cttcgccgac ggcggtagct cggggggcgg cgcggtaccg gcgatccgcg ccggaatcga
    10021 cgtggaaaag acgctgctcg aggagagcct ggcccggtac cgcgccgacg cgtcccggcg
    10081 cacggcgtta ctcggccgca tcgtcgagct gtacgcggag ctcgaccgcg tggtcggggc
    10141 gccggaggag gacgcgccgc gcagcgtcga gacgctgttg gatcagatcg cgacggccga
    10201 gggcgagcgc agccgcttgc tgctgtccga gcacgcgctg gtacgcgcga tcggaaccca
    10261 cgtccggcga atcgcgctgc tcgaagagca gctggtcgtt ctcgaggggc agcgcgagca
    10321 ggcggccggc gcgctcgcgg gcgagtggga cgtcgtcttg ctgccgctcg agcaacgcgg
    10381 tacgttccgg ttgatgcaga gcggcacgtt gatcagcgga acctacgagc tggccggtgg
    10441 c
//
LOCUS       DASPGN010000143         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_492270, whole genome shotgun sequence.
ACCESSION   DASPGN010000143 DASPGN010000000
VERSION     DASPGN010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492270"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VD788_03985"
     CDS             complement(<1..286)
                     /locus_tag="VD788_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HXV75455.1"
                     /translation="MMRLYNAALVPLRLAVSAWAALPLHRPEVARRWRERLARRLPEA
                     DSGGIWVHGASVGEARITAALSAAVRPRFPWIPVCVSATTATGRAQLPTPP"
     gene            451..2277
                     /locus_tag="VD788_03990"
     CDS             451..2277
                     /locus_tag="VD788_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXV75456.1"
                     /translation="MEQAAGKPRITDHPFFKRRGRIVFTLLAVLAFIGLAPLATVAWK
                     LIDINREALVTAQQEYQSLMATSVAHELDIHIEGQRSQVLRVAQTLGAAIGRGGYAQS
                     DQLRRVLEDVQGERMLYLRYTYFRSQDARSISAGQLDANLDPVFESGLEEAVQILSRT
                     AQDGGEETIVSRPILLETRPRRAALVISAPVISGTRFRGVLSALVDLSPVWQSVVERN
                     QSGHLIFALDEAGRVIASNAPARVVPGTDVSRSPMVVRFLKADGRAEETLPFVELDDG
                     AEQAYIGSYKTTGLGWGVFVQARLQDVYAPVNRMVDSITSRALLVIGLAVVAAIIFAR
                     TLSNPIKRLAAASRAFAAGDFSTRVEVRSGNELGELGFTFNTMAEEIESQIRQLKRAA
                     EENKELFLGTIRALAQAIDAKDPYTRGHSARVNRYSVVLARQLGMSDEDVADIHVASL
                     MHDVGKIGINDSILQKPGKLTADEFEVMKTHTTKGAQIMAPIRQMQRVIPGLRSHHER
                     WEGGGYPDGLTGAKIPFMARLIAVADAFDAMTTHRPYQTAMSFPKALERLNVLKGVAF
                     DERIVEAFNRAYRRGLIVPDPDPAVVENSDRRAPAATPPVVA"
BASE COUNT          381 a          797 c          777 g          343 t
ORIGIN      
        1 ccggcggcgt cgggagctgg gcccggccgg tcgccgtcgt cgccgagacg cagacgggga
       61 tccacggaaa gcgaggtcgc acggccgcgg acagcgcggc ggtgatccgc gcctcgccga
      121 ccgaagcgcc gtggacccag atcccgccgg agtcggcctc gggcagacgg cgtgccagcc
      181 gttcccgcca gcggcgggcg acctcgggcc ggtggagcgg cagtgcggcc caggccgaca
      241 cggcgagccg caaggggacg agagcggcgt tgtagagacg catcatcgct gacgggagga
      301 ctcgagaaaa cggcgccagt gtagtccagc ccgtggatcg ccgtcaacgc gttcgtgacg
      361 gcaagtctct ggctggcaag catttgccag ctccgccgcg gggaacctat attctgccac
      421 gccaaccgga tcgagggaga cgtccgcccc ttggagcagg ccgccggaaa gccccgcatc
      481 accgaccatc cgttcttcaa acggcggggg agaatcgtct tcacgctgct cgccgtgctg
      541 gccttcatcg gcctcgcgcc gctcgccacc gtcgcctgga agctgatcga catcaaccgc
      601 gaggcgctcg tcaccgcgca gcaggaatac cagtcgctga tggcgacgtc ggtcgcgcac
      661 gaactcgaca tccacatcga gggccaacgc tcccaggtcc tgcgcgtcgc ccagacgctc
      721 ggcgcggcga tcggtcgcgg cggctacgcc cagagcgacc agcttcgccg tgtgctcgag
      781 gatgtccagg gcgagcgcat gctgtacctg cgctacacgt acttccggtc ccaggacgcc
      841 cggtcgatct cggccgggca gctcgacgcc aatctcgacc cggtcttcga gtccggtctc
      901 gaggaggcgg tccagatcct gtcccgcacc gcgcaggacg gcggcgagga gacgatcgtc
      961 tcccggccga tcctgctcga gacgcggccg cgccgcgcgg cgctcgtcat ctcggctccg
     1021 gtgatctcgg gaacccgctt ccgcggggtg ctgtcggccc tcgtcgatct ctctcccgtt
     1081 tggcagtcgg tcgtcgaacg caaccagagc ggccacctga tcttcgcctt ggacgaggcc
     1141 ggccgcgtca tcgcgagcaa cgccccggcg cgcgtcgtgc cggggaccga cgtcagtcgc
     1201 tcgccgatgg tcgtgcgctt cctcaaggcc gacggccgcg cggaggagac gcttccgttc
     1261 gtcgagctcg acgacggcgc cgaacaggcg tacatcggct cgtacaagac gaccggtctc
     1321 ggctggggcg tcttcgtcca ggcgcgattg caggacgtct acgccccggt caaccggatg
     1381 gtcgacagca tcaccagccg ggcgctgctc gtcatcggac tggcggtcgt ggccgcgatc
     1441 atcttcgccc gcaccctgtc caacccgatc aagcgcctgg ccgccgcctc gcgtgcgttc
     1501 gcggccggtg acttctcgac gcgcgtcgag gtgcgttcgg gcaacgagct gggggagctc
     1561 ggcttcacct tcaacacgat ggcggaggag atcgagagcc agatccggca gctcaagcgt
     1621 gcggccgagg agaacaagga gctgtttctg ggaacgatcc gggcgctggc gcaggcgatc
     1681 gacgccaagg acccgtacac ccgcggacac tccgcccgag tgaatcgtta ctcggtcgtg
     1741 ctggcgcggc aactcggcat gagcgacgag gacgtggccg acatccacgt cgcgtcgttg
     1801 atgcacgacg tcggcaagat cggcatcaac gacagcatcc tgcaaaagcc gggcaagctc
     1861 accgccgatg agttcgaggt gatgaagacc catacgacca agggcgcgca gatcatggcg
     1921 ccgattcgtc agatgcaacg cgtgatcccc ggcctgcgtt cgcaccacga acggtgggag
     1981 ggcggaggct atcccgacgg tctgaccggc gcgaagatcc cgttcatggc ccgactgatc
     2041 gccgtcgccg acgcgttcga tgcgatgacg acccatcggc cgtatcagac ggcgatgtcc
     2101 ttccccaagg cgctcgagcg gctcaacgtt ctcaagggag tcgcgttcga cgagcggatc
     2161 gtcgaggcgt tcaaccgtgc ataccgacga gggttgatcg ttcccgaccc cgacccggcg
     2221 gtcgtggaga acagcgaccg ccgagcgccg gccgcgacgc cgcccgtcgt cgcctgagtc
     2281 tcagagcgcg atcttcga
//
LOCUS       DASPGN010000144         5589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_609897, whole genome shotgun sequence.
ACCESSION   DASPGN010000144 DASPGN010000000
VERSION     DASPGN010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609897"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VD788_03995"
     CDS             <1..621
                     /locus_tag="VD788_03995"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXV75457.1"
                     /translation="AQRDPRKTPVGIDELARLEALARREIHEQVRDGGSVGTAWRDYF
                     HIILGGASVPAASQSRLIDSLWEAHRRFGLWTTPIDDGPQVVAQVRKRFTIGVVSNAE
                     GNVARDLDAAGYAGQFGVVIDSHVVGVEKPDPAIFRIALERLGKQAQTTVFVGDLPAV
                     DVAGARAAGITPILLDRHDLYPDTGSHRIRSIGDLPRLLDTAPRSS"
     gene            complement(618..1445)
                     /locus_tag="VD788_04000"
     CDS             complement(618..1445)
                     /locus_tag="VD788_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75458.1"
                     /translation="MRKFLKLSRLVPIGMLLLAPAAASAQDEPATAPRVDSTVTSSRE
                     RLFLNFSEDAMITDEQWWEGWLQYDDRDEVDRTILFGQAAFQIWVNTEIGVRVGFGRT
                     DNAPPLDEGNGATDLEAWGKYYWNLGNDRTELAAGVILTVPSGDDSVGLGFDAFALKG
                     FGAVRYRLSTVILTGNVGLQLNEDGQTLNSGELQGETAFSVGAGVIAPWSDSFSFVGE
                     LTWEQSRFEEIDDGEAQLLGGLNLRLTNRGILRPAVAVGLQDGSPDWQIYLGYAYVF"
     gene            1626..2138
                     /locus_tag="VD788_04005"
     CDS             1626..2138
                     /locus_tag="VD788_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV75459.1"
                     /translation="MDDRGFERAVIAHKDRVFAFATAMLKDGAEAQDVSQESLIRLWQ
                     NRNAVEEDGTRPWLMRTAHNLCIDRLRKRKVRGEIAEGDTVVASQVDHTPGPQQAAES
                     SEIRANLQSVLGELPAVDRAVLVMREIQGLPYEEIAGVLGLPLGTLKARLHRARDRLR
                     SRLVRAGVTP"
     gene            2135..2689
                     /locus_tag="VD788_04010"
     CDS             2135..2689
                     /locus_tag="VD788_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV75460.1"
                     /translation="MTNDGSVRRIECARAMDRVHRRLDGEALGPADVRALASHLAECV
                     DCRQANGELGQIQLAMRELGEQRFPDGGLEQVWEQTTRRTGRARRSAWWAAAAAIVVV
                     SAWLALWQGGRQDPVAPSDAELERAAREARLVLRLAAQAIDRTERAAFADVLGGQVSP
                     ALRKVPIRWPAAEPGRSPKDGNDA"
     gene            2682..3242
                     /locus_tag="VD788_04015"
     CDS             2682..3242
                     /locus_tag="VD788_04015"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HXV75461.1"
                     /translation="MREFALPSIALLLVTAFAGGAGASGPTPPGFVDGTRLVDVLGDD
                     NVRLEVTLGPTLLRPLLLADPTLQEVVGELESIYALIVDLSDPETARRAAAEAGAIEK
                     RLVGEGWERVARVRSDGGERIQVLVRKADGDDAHLSGLTVIVIDASGDEPIFVFANIT
                     GLIDLGAIERLGDLDFPGITGLDLEP"
     gene            3244..3891
                     /locus_tag="VD788_04020"
     CDS             3244..3891
                     /locus_tag="VD788_04020"
                     /inference="COORDINATES: protein motif:HMM:NF025426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HXV75462.1"
                     /translation="MGERRCWLLLALAVAIGCGTGCVRLVGPEDIRDDLSRQAGVKLR
                     QQTGFTVTRSGIWLARQFVDPEEVPLKGIRRVEIGIYEVRGLKKSLEAQRPLDLTIFR
                     DWAPVVTVRDHGEEVSVLSRTDEQGEIRGIMIVVAEEDEWVLVRVRGRLDRIFEAALE
                     QAFADVHRGDLYAKTRVERGMEEAGPADDRPEPAAPAVDGGTEALQRPTSFKPQE"
     gene            complement(3858..4595)
                     /locus_tag="VD788_04025"
     CDS             complement(3858..4595)
                     /locus_tag="VD788_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HXV75463.1"
                     /translation="MVASLPAVVVSVLLAVTTRVPVEPVERFDHSPFDAVLAEVVSGE
                     SVDYAKLRELHRNALRSYLDRLAEIDPAGMDDHERLALYLNLYNASMLAAVVDRYTPG
                     YSPADDDFAVFDEPRVRLAGRRVTLNDLEHRLIRREFDDPRIHAALVCAARSCPPLAN
                     RAYRGEDLDEMLDRRMRSFVNDSDRNRIDAAGKTLRLSSVFDWYADDFGGRDRLSAYV
                     DRFTEVDVSGFDVEFLPYSWGLNDVGR"
     gene            complement(4606..4899)
                     /locus_tag="VD788_04030"
     CDS             complement(4606..4899)
                     /locus_tag="VD788_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75464.1"
                     /translation="MADSIEVTCPCCATRLVVDVATGEVLSLVRPPDDHEQSFDAAMD
                     AVHKSADRREEAFSRAFKRTQNLDDLLQKKFDEARKKAEQEPDKKHRNPLDFD"
     gene            complement(4904..5245)
                     /locus_tag="VD788_04035"
     CDS             complement(4904..5245)
                     /locus_tag="VD788_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HXV75465.1"
                     /translation="MSDRRDDEGPPEEIAVPIEDALDLHAFRPADVRSVVEAYLDEAV
                     EAGFEEVRLIHGRGIGVQREIVRTLLARHPAVVEYRDAPAERGGWGATVVRLRRFDAP
                     GSPRENDHSVE"
     gene            complement(5242..>5589)
                     /locus_tag="VD788_04040"
     CDS             complement(5242..>5589)
                     /locus_tag="VD788_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75466.1"
                     /translation="ERAGDARQAIPRPIALPPGAFDTDAVSEITDPDGWGRVAGLDPD
                     RIPPYDDARRLVLVGPRSPAVDCTGAEVIATDDVWVIRFRPAAEPSEATACLFLLPRD
                     GRRSRVVVDGSAP"
BASE COUNT          910 a         1866 c         1951 g          862 t
ORIGIN      
        1 gctcagcggg acccgcgaaa gaccccggtc ggcatcgacg agctggcgcg cctcgaggcc
       61 ctggcgcggc gggagatcca cgaacaggta cgcgacggcg gcagcgtcgg cacggcgtgg
      121 cgcgactact tccacatcat cctgggtggc gcgtccgtcc ccgccgccag ccaatctcgc
      181 ctgatcgatt cgctctggga agcccaccga cgattcggcc tttggacgac gccgatcgac
      241 gacggaccgc aggtggtcgc gcaggtccgc aagcggttca ccatcggcgt ggtgagcaac
      301 gcggagggca acgtcgcccg ggatctggac gcggccgggt acgctggcca gttcggcgtc
      361 gtcatcgatt cacacgtcgt cggcgtcgag aagccggacc cggcgatctt ccgcatcgcg
      421 ctcgagcgcc tcggcaagca ggcgcaaacg accgtcttcg tcggcgatct accggccgtc
      481 gacgtcgccg gagcgcgcgc cgcgggaatc acgccgatcc tgctcgaccg ccacgacctc
      541 tatcccgaca cgggatccca tcgcatccgg tcgatcggtg acctcccgcg gctactcgat
      601 accgcgccgc gctcgagcta gaagacgtaa gcgtagccga ggtagatctg ccaatcgggc
      661 gagccatcct gcaggccgac ggcgaccgcg ggtcggagga tgccacgatt ggtcagacgc
      721 aggttgagac cgccgagcag ctgcgcctcg ccgtcgtcga tttcctcgaa tcgactttgc
      781 tcccacgtca actcgccgac gaacgaaaac gagtcgctcc acggcgcgat cacaccggcg
      841 ccgaccgaga aggccgtctc gccctggagc tctcccgaat tcaacgtctg gccgtcttcg
      901 ttcagctgca agccgacgtt gccggtcagg atcaccgtgc tcaagcggta gcgcaccgcg
      961 ccgaagccct tcagcgcgaa cgcatcgaag ccgagcccga ccgagtcgtc gccgctcggc
     1021 acggtcagga tcaccccggc ggcgagttcc gttcggtcgt tacccaagtt ccagtagtac
     1081 ttgccccagg cctcgagatc ggtcgcgccg ttgccctcgt cgagcggtgg ggcgttgtcc
     1141 gtgcggccga agccgacccg aacaccgatc tcggtgttga cccagatctg gaaggccgcc
     1201 tgcccgaaca ggatcgtccg gtcgacctcg tcccggtcgt cgtactgcag ccaaccttcc
     1261 caccactgct catcggtgat catcgcgtct tcagagaaat tgaggaacag ccgctcgcgg
     1321 ctcgacgtga cggtggagtc gacccggggg gccgtcgccg gctcgtcctg ggccgaagct
     1381 gcagccggag cgagcagcaa cattccaatc ggtaccaagc ggctcagttt caagaacttt
     1441 cgcattacaa tcgtctcctc tgcgagtcgc cgtttcggga ataccgggct ccggtgggta
     1501 ccgctccagc cctcgacacg gcgccggccg ccgccgtgag cggggaccat tgtgggaagc
     1561 cgtgccgggg ctgtcaatgc agtcgaggca actttcggag aagctgcccg ttgttagggt
     1621 gtgcgatgga tgaccggggg ttcgaacggg ccgtgatcgc gcacaaggac cgggtgttcg
     1681 cattcgcgac ggcgatgctg aaggacgggg cggaggccca ggacgtgtcg caggaatcgc
     1741 tgatcaggtt gtggcagaat cgaaacgcgg tcgaggaaga cggaacgcgg ccgtggctga
     1801 tgcgcaccgc tcacaacctg tgtatcgatc ggctgcgcaa gcggaaggtc cgcggcgaga
     1861 tcgcggaggg cgacaccgtc gttgcatcgc aggtcgacca cactcccggt ccgcagcagg
     1921 cggcggagtc gagcgaaatc cgagcgaacc tccaatcggt gctcggcgag ttgccggccg
     1981 tcgaccgagc cgtactggtc atgcgcgaga tccagggctt gccgtatgag gagatcgccg
     2041 gtgtgctcgg cctcccgctc gggacgctga aggcaaggtt gcaccgggcg cgcgatcgtc
     2101 tgcggagccg gctggtccgg gcgggagtga ccccgtgacg aacgacggca gcgtccgccg
     2161 catcgaatgc gcccgcgcga tggatcgcgt acatcgccgt ctcgacgggg aagctctcgg
     2221 tccggccgac gtgcgggccc tcgcctcgca cctcgccgag tgcgtggatt gccggcaggc
     2281 gaacggcgag ctgggacaga tccagctcgc catgcgcgag ctcggcgagc aacgattccc
     2341 cgacggtggg ctcgagcagg tctgggagca aacgacgcgg cggaccggcc gcgcccggcg
     2401 atccgcatgg tgggcggcgg cggcggcgat cgtcgtggtc tcggcctggc tcgcgctgtg
     2461 gcagggggga aggcaggatc cggtcgcgcc gtccgatgcc gaattggaac gggcggcccg
     2521 cgaggcccgt ctcgtgctcc gtctcgccgc ccaggcgatc gaccggaccg agcgagccgc
     2581 gttcgcggac gtactcggcg gtcaggtctc gccggcgctg cggaaagtgc cgatccgttg
     2641 gcccgcggcc gagccgggac gaagcccgaa ggacggcaac gatgcgtgag ttcgccctac
     2701 cgtcgatcgc cctgctgctg gtcacggcat tcgccggcgg cgcgggcgcg tccggtccga
     2761 cgccaccggg cttcgtcgac gggacgcggc tggtcgacgt actcggtgac gacaacgttc
     2821 ggctcgaggt gacgctgggt cccacattgc tccgcccgct gctcctggcg gacccgacgt
     2881 tgcaggaagt cgtcggggag ctcgagtcga tctacgctct gatcgtcgac ctgagcgacc
     2941 ccgaaacggc gaggcgagcc gcggcggagg ccggcgcgat cgagaaaagg ctggtcggcg
     3001 agggttggga gcgggtggcg cgcgtacgat ccgacggcgg ggagcgcatc caagtattgg
     3061 tgcgcaaggc cgacggtgac gacgcacacc tcagcggact gacggtgatc gtcatcgacg
     3121 cgagcggcga cgagccgatc ttcgtgttcg ccaacatcac cggtttgatc gatctcggtg
     3181 cgatcgagag actcggggac ctggatttcc ccggaatcac gggtctcgat ctggagcctt
     3241 gagatgggtg aacgacgctg ttggttgctg ttggcgctgg cggtcgcgat cggctgcggc
     3301 acgggttgcg tgcggctggt cggtccggag gacattcgcg atgacctcag ccggcaggcc
     3361 ggcgtgaagc tccgccaaca gacgggcttc acggtgaccc gctcggggat ctggctcgcg
     3421 cggcagttcg tcgacccgga ggaggttccg ctcaagggca tccggcgcgt cgagatcggg
     3481 atctacgagg ttcgcggtct gaaaaagagc ctcgaagcgc aacgtccgtt ggacctgacg
     3541 atcttccgcg actgggctcc ggtggtcacc gtgcgcgatc acggcgaaga agtgtccgtg
     3601 ctgtcccgca cggacgagca gggagagatt cggggcatca tgatcgtcgt cgcggaagag
     3661 gacgagtggg tgctggtccg ggtccgtggc aggctcgacc ggatcttcga agcggcgctc
     3721 gagcaggcgt tcgccgacgt ccaccgcggc gacctgtacg ccaagacgcg cgtggagcgc
     3781 gggatggagg aggcgggccc ggccgacgac cgacccgagc ccgccgctcc ggcagtcgac
     3841 ggcgggaccg aggcacttca gcgaccgacg tcgttcaagc cccaggagta aggcaggaac
     3901 tcgacgtcga agcccgagac gtcgacctcg gtgaatcggt cgacgtaggc cgagagccgg
     3961 tcgcgccccc cgaagtcgtc ggcgtaccag tcgaagacgc tcgacaaccg cagcgtcttg
     4021 ccggccgcgt cgatccgatt gcggtcggaa tcgttgacga aggacctcat ccgtcgatcg
     4081 agcatctcgt cgaggtcttc gccgcgatag gcccgattcg ccagcggcgg acaggaacgg
     4141 gccgcgcaca cgagagccgc gtggatccgc gggtcgtcga attcgcggcg aatcagccgg
     4201 tgctcgaggt cgttcagggt gaccctcctg ccggccaacc gcacgcgcgg ttcgtcgaaa
     4261 accgcgaaat cgtcgtcggc cggcgaatag cccggggtgt acctgtcgac cacggccgcc
     4321 agcatcgaag cgttgtagag gttgaggtag agcgccaggc gctcgtgatc gtccatcccg
     4381 gccggatcga tctcggccag ccggtcgagg taggagcgca gggcgttccg gtggagctcc
     4441 cgcagcttcg cgtagtcgac cgactcgccc gagaccacct cggccagcac cgcgtcgaac
     4501 ggcgagtggt cgaaccgctc gaccggctcg accgggactc tcgtcgtgac ggcgagcagc
     4561 acggagacca cgacggcggg gaggcttgcg accatggccc gagggtcagt cgaagtcaag
     4621 cgggttgcgg tgcttcttgt ccggctcctg ctcggccttc ttccgcgctt cgtcgaattt
     4681 cttctgcagc aggtcgtcga ggttctgagt ccgtttgaac gcgcggctga acgcctcttc
     4741 cctccgatcg gcgctcttgt ggactgcgtc catcgccgca tcgaacgact gttcgtgatc
     4801 gtccggcggc cgcactaggg acaacacttc accggtcgcc acgtcgacga ccaggcgggt
     4861 cgcacagcag ggacaggtca cctcgatcga atcggccatc cgctcactcc accgagtgat
     4921 cattctcccg cggcgagccc ggggcgtcaa accggcgcag gcggacgacg gtcgcacccc
     4981 agccaccgcg ttcggcgggc gcgtcgcgat actcgacgac ggccgggtgc cgggcgagca
     5041 gcgttcgtac gatctcgcgc tgcacgccga tgccgcggcc atggatcaac cgcacctctt
     5101 cgaacccggc ctcgaccgcc tcgtcgaggt aggcctcgac gacggaccgt acgtccgccg
     5161 gccggaaagc gtgcaggtcg agcgcgtcct cgatcggaac cgcgatttcc tccggcgggc
     5221 cctcgtcgtc gcgtcggtcg ctcatggcgc cgacccatcg acgacgacgc gcgatcgccg
     5281 gccgtcccgc ggcaaaagga agagacaggc ggtcgcctcg ctcggctcgg cagcgggccg
     5341 gaagcggatc acccacacgt cgtccgtcgc gatcacctcc gcgcccgtac aatcgactgc
     5401 cgggctccgc ggcccgacga gcacgagacg acgcgcgtcg tcgtacggcg gaatccggtc
     5461 cggatcgagg ccggcgacgc gaccccaacc gtcgggatcg gtgatctcgc tcaccgcgtc
     5521 ggtgtcgaac gcgccggggg ggagcgcgat cggccgcggg atcgcctgcc gcgcgtcgcc
     5581 cgctcgttc
//
LOCUS       DASPGN010000145         3738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_375214, whole genome shotgun sequence.
ACCESSION   DASPGN010000145 DASPGN010000000
VERSION     DASPGN010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375214"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VD788_04045"
     CDS             complement(<1..900)
                     /locus_tag="VD788_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75467.1"
                     /translation="MNCRQFRLDLERHIAGEPSRDGLGPLAAHCAACEPCGRLLSLHT
                     RLSDLGRALSRAAAAVDLDDTRSRLMERVIARVAAGQPARPAHGTLVLRLAVVALLAL
                     TLIASGALASWVALSGPDRGLPRLLSSISRDAAANRSLLDVEDSPYSYSNVIFRTLGD
                     GRLELDFDVTRHVRVAEPVHSELVKDVLVHSLIGPSSTGARLKAISIAAEVMDTKVRD
                     SLILAMHNDANLAVRLRALEILSAQAADERIEQAVLHTLREDGAVQMRLEALDYLATR
                     PGRTGSIRRVIEQLDDANYAALMA"
     gene            complement(897..1445)
                     /locus_tag="VD788_04050"
     CDS             complement(897..1445)
                     /locus_tag="VD788_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV75468.1"
                     /translation="MPLTDEQLIGEVRAGSSAAFEQVVRRYERLVFHVAFGVTADRDA
                     ALDVSQNVFLRLHAKLPAVHAVRGLKPWLTRVAVNESINWNRAVRRRPVAELNDRLTV
                     GGPSPEERVSRRETWDMLRRSLESLGTRHRLAVVLRYVDGMPIREIATALGCNEGVAK
                     SILFRSLKRMRGHLARTEEIAK"
     gene            1653..2486
                     /locus_tag="VD788_04055"
     CDS             1653..2486
                     /locus_tag="VD788_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75469.1"
                     /translation="MESTSLLGIGGHPAGHAATLRRDRWWVEPAITVGVLLAFAVYAS
                     WAAFQGNHYYVAPYLSPFYSPVLWTDLSVAGAAPAWHAAFGTLPVWWPGWAPYSPAFL
                     ILVFPGAFRFTCYYYRKAYYRSFAGSPPACAVTPMAPAGKPYRGETAWLLFQNLHRYA
                     LYFALLYLPILALDAIAAFVRDGRVGIGVGSLVLTLNVVLLSGYTLGCHSFRHLVGGR
                     VDCLSCGKQTVRFRWWKMSSWLNLRHPQFAWLSLFGVALTDLYVRLVSMGVVRDVNTW
                     D"
     gene            2494..>3738
                     /locus_tag="VD788_04060"
     CDS             2494..>3738
                     /locus_tag="VD788_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXV75470.1"
                     /translation="MNVNEIQTIEHDVVVIGAGGAGLRAAIESSARGMSTGLVTKSLL
                     GKAHTVMAEGGMAAALGNVDRRDHWKIHFRDTVRGGKFLNVWRMAELHAKEAPERVRE
                     LERWGAVFDRTRDGLISQRNFGGHRYPRLAHVGDRTGLELIRTLQDHGVHQGFDVHME
                     CTAVQLLKDGGRIAGVAAYWRETGRFVLFRCRAVVLATGGAGKAWRVTSNSWEYTGDG
                     LAMAYEAGAELMDLEFTQFHPTGMVWPLSVRGILVTEGVRGDGGVLLNSEGKRFMFDY
                     IPTRFAGETAQTVEEAERWLAGADGARRPPELLTRDVVARAITNEVKQGRGSPHGGVL
                     LDIASRRQADFIRRKLPSMYHQFKELAEVDITREPMEVGPTLHYFMGGIRVDAESQQT
                     CVDGLFACGECAAGLHGANRLGG"
BASE COUNT          580 a         1277 c         1281 g          600 t
ORIGIN      
        1 cgccatcaac gcggcatagt ttgcgtcatc gagttgttcg atgacacggc ggatcgaccc
       61 cgtacgaccg ggtctcgtcg ccaggtagtc gagcgcctcg agccgcatct ggaccgcgcc
      121 gtcctcgcgc aacgtgtgca ataccgcctg ctcgatccgc tcgtcggcgg cctgcgccga
      181 caggatctcg agcgcccgta gacggaccgc caggttcgcg tcgttgtgca tcgccagaat
      241 cagcgagtcg cgcactttgg tgtccatcac ctccgcggcg atcgagatcg ccttgaggcg
      301 cgctccggtg ctcgacggcc cgatcagcga gtggaccagc acgtccttga ccagctccga
      361 gtgcaccggc tcggccaccc gcacgtgccg cgtgacgtcg aagtcgagct cgagccgacc
      421 atcgccgagc gtgcggaaga tgacgttgga atagctgtag ggagagtcct cgacgtcaag
      481 caggctacgg ttggcggccg cgtcgcgcga gatcgagctc aacaaccgcg gcaatccacg
      541 atcgggaccg gacagcgcga cccagctggc cagcgccccg ctcgcgatca acgtcagcgc
      601 gagcagggcg acgaccgcca gtcgcagcac gagtgtcccg tgagcgggtc gcgccggctg
      661 gccggcagcc accctcgcga tcacgcgctc catcagccgc gagcgtgtat cgtcgaggtc
      721 gaccgcggcg gcggcgcgcg acagcgcccg gccgaggtcc gacagacgcg tgtgcagcga
      781 gagcaacctg ccgcacggct cgcacgccgc gcagtgcgcg gccagcggcc cgagaccgtc
      841 gcgcgacggt tcgccggcga tatggcgctc gaggtcgagc cggaactgtc tgcagttcat
      901 ttcgcaatct cctccgtccg agcaaggtgt ccgcgcattc tcttcagact tcgaaagagg
      961 atgctcttcg cgactccctc gttgcagccg agcgccgtcg cgatctcgcg gatcggcatc
     1021 ccgtcgacgt agcgcagcac caccgccaga cgatggcgcg tgccgagcga ctcgagcgag
     1081 cggcggagca tgtcccaggt ctcccgccga ctgacccgct cttccggcga cggaccgccg
     1141 accgtcaatc ggtcgttcag ctcggcgacc gggcggcgtc gtaccgcgcg gttccaattg
     1201 atgctctcgt tgaccgcgac ccgcgtgagc cacggcttga gacctcgaac cgcatgaacc
     1261 gccggcagct tggcgtgcaa gcgcaggaag acgttctgcg aaacgtcgag tgcggcatcc
     1321 cgatcggcgg tgacgccgaa cgcgacgtgg aacaccagac gctcgtagcg ccgcacgacc
     1381 tgttcgaagg ccgcgctcga gccggcgcgc acttcaccga tcagctgctc gtcggtcaag
     1441 ggcaagcggc gagacctccg ctggattaag acaccgcgcg cgggagaaag ttagcaacgg
     1501 agcatcgaat ccgtcggaaa agagccgctc cgtccgtggc aaatcggccg ccgaaccgcg
     1561 tcagcgaccg acacggtcgc ttgcttggcc gacggatttc cccgtatgat ccgcgttcgg
     1621 ttcgggccgg cggcccgcac ggaggaggcg cggtggaatc gacttcgttg ctcggtatcg
     1681 gcggccaccc ggcgggccac gcggcgacgc tgcggcgtga tcggtggtgg gtcgaaccgg
     1741 cgatcacggt cggcgtgctg ctggcattcg cggtctacgc cagctgggcg gcgtttcagg
     1801 ggaaccacta ctacgtcgcg ccgtacctca gcccgttcta ctccccggtg ctgtggaccg
     1861 acctgtcggt tgccggcgcc gcgccggcct ggcatgcggc gttcggcacg ctgccggtct
     1921 ggtggcccgg ctgggcgccc tactcgccgg cgttcctgat cctggtcttc cccggcgcct
     1981 tccgcttcac ctgctattac taccgcaagg cgtactaccg ctcgttcgcc ggctcgccgc
     2041 cggcgtgtgc ggtaacgccg atggcgccgg ccgggaaacc gtaccggggt gagacggcct
     2101 ggctgctgtt ccagaacctc caccgttacg cgctctactt cgccctgctc tacctgccga
     2161 tcctcgcgct cgacgcgatc gccgcgttcg tccgcgacgg tcgggtcggc atcggcgtcg
     2221 gcagcctggt gctcacgctc aacgtcgtcc tgctgagcgg ctacacgctc ggctgccact
     2281 ccttccggca cctggtcggc gggcgggtcg attgcctgtc ctgcggaaag cagaccgtcc
     2341 gtttccgttg gtggaagatg tcgagctggc tcaacctccg ccacccgcag ttcgcctggt
     2401 tgagtctgtt cggcgtcgcg ttgaccgatc tctacgtgcg gttggtctcg atgggcgtcg
     2461 tgcgtgacgt caacacgtgg gattgactcg ccgatgaacg tcaacgagat ccagacgatc
     2521 gagcacgacg tggtggtcat cggcgccggc ggggccggat tacgcgccgc gatcgagagc
     2581 agcgcgcgcg ggatgagcac cggcctcgtc accaagagcc tgctcggcaa ggcgcacacg
     2641 gtgatggcgg agggcgggat ggcggccgcg ctgggcaacg tcgaccgacg cgaccactgg
     2701 aagatccact ttcgcgacac cgtgcgcggc ggcaagttcc tcaacgtctg gcgcatggcc
     2761 gagcttcacg ccaaggaagc gccggagcgc gtgcgtgagt tggagcgctg gggtgcggtc
     2821 ttcgaccgta cgcgggacgg cctgatcagc cagcgcaact tcggcggcca ccgctatccg
     2881 cgactggccc acgtcggcga ccggaccggg ctcgagttga tccgcaccct gcaggatcac
     2941 ggagtccacc aggggttcga cgtgcacatg gagtgcaccg cggtccagct gctcaaggac
     3001 ggcgggcgga tcgccggcgt cgcggcctac tggcgcgaga cggggcgatt cgtcctgttc
     3061 cggtgccggg cggtggtgct cgcgaccggc ggcgccggca aggcctggcg tgtgacgagc
     3121 aactcgtggg agtacaccgg tgacggcctg gcgatggcct acgaggccgg cgccgagctg
     3181 atggacctcg agttcaccca attccacccg accgggatgg tctggccgct gtcggtacgt
     3241 ggcatcctgg tgaccgaggg cgtgcgcggc gacggcggcg tgctgttgaa ctccgaaggc
     3301 aagcggttca tgttcgacta catcccgaca aggttcgctg gcgaaacggc gcagaccgtc
     3361 gaggaagccg agcgctggct ggccggggcg gacggcgccc gtcgcccgcc cgagctgctg
     3421 acccgcgacg tcgtcgcgcg tgcgatcacc aacgaagtca agcaaggacg gggctcgccg
     3481 cacggcggcg tgcttctcga catcgcctct cgtcgccaag ccgacttcat ccggcgcaag
     3541 ctaccgtcga tgtaccacca gttcaaggaa ctggccgaag tcgacatcac gcgcgagccg
     3601 atggaggtcg ggccgacgct gcactacttc atgggcggga tccgtgtcga cgccgaatcg
     3661 cagcagacgt gcgtcgacgg tctgttcgcc tgcggcgagt gcgctgccgg gttgcacggc
     3721 gcgaaccgcc tgggcggc
//
LOCUS       DASPGN010000146         5159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1546353, whole genome shotgun sequence.
ACCESSION   DASPGN010000146 DASPGN010000000
VERSION     DASPGN010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1546353"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="VD788_04065"
     CDS             <1..1099
                     /locus_tag="VD788_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75471.1"
                     /translation="EVPPLAELPLGSEALVERLAPVVARKAGREVERDSRKNPRDMHA
                     RPYHDIPLVVHGRTFFDLRERVATALFRYPDYRDWALDRLADEARAELEALERRYRTR
                     IPGASDEAIERAVRLSQDGRRIRERAVNPTPRDLSRHLAAWLGDLPAQRLLADWEARR
                     IDELVRRPGPTPADDGFVDQWLALRQLLFVPSYARELTPLVLTRSPDGDRIGFYRIVL
                     PRPGWFYSRVRNYRDHAGASDLPFDLIPDRPAAFETMRELLADQPDLVRHLAAAGYRV
                     RAIDYRSRLKPRKQRPDRALDELRVALELAVDERLISLELDLRTAASPTRPCPLLALR
                     ATSQGDQPAAGWLARLQSSTLSRSNNARYSP"
     gene            complement(1051..2169)
                     /gene="msrA"
                     /locus_tag="VD788_04070"
     CDS             complement(1051..2169)
                     /gene="msrA"
                     /locus_tag="VD788_04070"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXV75472.1"
                     /translation="MSYGSRKVFSTLVAGAVLSSILACSGSDAGDGGAGLELATFAGG
                     CFWCMEPPFEKLAGVRSVVSGYTGGKEKQPSYEQVSSGATGHAEAVQVRYDPALVDYE
                     TLVRVFWSSIDPTDAGGQFADRGSQYRTAIFYHDEKQREIAERSKQELGASGRFDRPL
                     VTPIEPASRFYPAEEYHQDYYKKHPGQYESYRWNSGRGPFLARVWGEVPPPSGKSERR
                     DEEGFVKPSDEELRKQLTPQQYRVTQEDGTEPAFDNEYWAHKGEGIYVDVVSGEPLFS
                     STHKYDSGTGWPSFYRPLEQDNIVEHEDDKLTMRRIEVRSAAGDSHLGHLFPDGPRPT
                     GLRYCINSAALRFIAKDELERQGYGEYLALFERDKVED"
     gene            complement(2255..2971)
                     /locus_tag="VD788_04075"
     CDS             complement(2255..2971)
                     /locus_tag="VD788_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV75473.1"
                     /translation="MFDMTDRVVMIAGAAGNLGQAGVRRFRRAGARVVAVDHSEGRLP
                     GIFGDLTGGGELLCVEGVDMTRFDEVAAAVDRVLRSFGRIDALFNTVGGFAAAEVAGD
                     ESPDTWRRMLRMNLDTTLNGCRAVVPSMVERGRGRIVNTAARAGFGAPAGLAAYAASK
                     SAVLRLTESLAHEVKGAGVNVNCVVPGTIDTPQNRAAMPDADPSTWVEPEAVADVAVF
                     LASEAARSVTGAAVPVYGRL"
     gene            3030..3290
                     /locus_tag="VD788_04080"
     CDS             3030..3290
                     /locus_tag="VD788_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75474.1"
                     /translation="MSEQVATAISVGLALYFGAGVLFAIPFVIRGVDRIDEVARHSGW
                     GFRLMILPGSIALWPILLRRWIGGAQPGEESNAHRLAARKSR"
     gene            3287..3463
                     /locus_tag="VD788_04085"
     CDS             3287..3463
                     /locus_tag="VD788_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75475.1"
                     /translation="MIRSLRRRHRLLVVAIGTATGLLLVLGLLSRRPPPRVPSLGELD
                     RAESAGEAAPVVDR"
     gene            3460..4512
                     /locus_tag="VD788_04090"
     CDS             3460..4512
                     /locus_tag="VD788_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXV75476.1"
                     /translation="MSVAYRAVLWNRQKRIYDTVIAIGVVSYLVSFVGLGLAVRPQAT
                     VETLIIRASGTLGLLLLHIILSIGPLCRLDARFLPLLYNRRHLGVTMFLVAALHATFA
                     LFQFHALGDVHPLVSLLSSNTRFGSSSQFPFELLGLGALSILSLMAATSHDFWLRNLS
                     APVWKSLHMLVYLAYALLVLHVVLGVLQDETDPLLVGVLVFGFVWVVGLQVAAGFGER
                     RADGELSAALADGYMPACSVDEIRIGRARVVCLGGERVAIFRYDDKISAVSNVCQHQN
                     GPLGEGRIVDGCITCPWHGYQYLPDSGASPPPFNEKIPTFNVRVAGGRVLVDPRPNPP
                     GTRVEPARVGGGARGG"
     gene            4505..>5159
                     /locus_tag="VD788_04095"
     CDS             4505..>5159
                     /locus_tag="VD788_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75477.1"
                     /translation="MADGRSDFYIGYEPRGPRRLARRMRLVIALLFALALALAALLVG
                     AQRSFDGGVFEFGVDRQLVGTLVEQPYPLLRVPGDDGAPARTHLLVGHGKHGAGPLVA
                     GLDGRVVRVSGSPIHHGRHRMIEIHRVETIDAVGGAMPSERAALGTMTLAGEIVDSKC
                     HLGVMKPGRGKTHRACAVRCISGGSPPLLRVEDRTGRVELFLLVDEAGASVNRRVLSM
                     "
BASE COUNT          691 a         1842 c         1764 g          862 t
ORIGIN      
        1 cgaggtgcca ccgctggccg agctgccgct ggggagcgaa gcgctggtcg agcggctcgc
       61 gccggtcgtc gcgcgcaagg ccgggcggga ggtcgagcgc gactcgcgca agaatccgcg
      121 cgacatgcac gcccgtccct atcacgacat cccgctcgtg gtgcacggcc gcacgttttt
      181 cgatctgcgc gagcgggtgg cgacggcgtt gtttcgctac cccgactacc gtgactgggc
      241 gctcgaccgg ttggcggacg aggcgcgtgc cgagctcgag gcgctcgagc gtcgctaccg
      301 cacgcgaata cccggcgcgt ccgacgaggc gatcgagcgg gccgtccggt tgtcgcagga
      361 cggcaggcgc atccgcgaac gggcggtcaa cccgacaccg cgcgacctgt cgcgacacct
      421 cgcggcgtgg ctcggcgatc tcccggcgca acgcttgctc gccgactggg aggcccgccg
      481 catcgacgag ctggtccgcc ggccgggccc gaccccggcc gacgacggct tcgtcgacca
      541 gtggttggcg ctgcgccagt tgctgttcgt gccgtcctac gcccgcgagc tgaccccgct
      601 cgtgctcacc cgctcgcccg acggcgaccg catcggcttc tatcgcatcg tgctgccgcg
      661 cccgggctgg ttctactcgc gcgtacggaa ctaccgcgac cacgcggggg cgagcgatct
      721 gccgttcgat ctgataccgg accggccggc ggcgttcgag acgatgcggg agttgctcgc
      781 cgaccagccc gacctcgtgc ggcacctggc ggcggccggg taccgagtcc gtgcgatcga
      841 ctaccgctca cggctaaagc cgcgcaagca gcgccccgac cgggcgttgg acgagttgcg
      901 cgtcgcgctc gagctggcgg tcgacgagcg actcatctcg ctcgagctcg acctgcgcac
      961 ggccgcgagt ccgacgcggc cgtgcccgct gctcgcgctg cgcgcgacca gccaaggcga
     1021 ccagccggcg gcggggtggc tggcgcgcct tcagtcctcg accttgtcgc gctcgaacaa
     1081 cgcgagatac tcgccgtagc cctgacgttc cagctcgtct ttggcaatga aacgcaaggc
     1141 agcgctgttg atgcagtagc gcaagccggt cggccggggt ccgtccggga agaggtgacc
     1201 gagatggctg tcgcccgccg cgctgcgtac ctcgatccgc ctcatcgtca gcttgtcgtc
     1261 ctcgtgctcg acgatgttgt cctgttcgag cggccgatag aagctgggcc agccggtccc
     1321 gctgtcgtac ttgtgagtcg agctgaacag cggctcgccg ctgacgacat cgacgtagat
     1381 cccctcgccc ttgtgagccc agtactcgtt gtcgaacgca ggctcggtgc cgtcctcctg
     1441 ggttacacgg tactgctggg gggtcagttg cttcctgagt tcctcgtcgc tcggtttcac
     1501 gaaaccctcc tcgtcgcggc gctcggactt gccgctgggc ggtggcacct caccccacac
     1561 gcgagccagg aacggcccgc gacccgagtt ccagcgatat gactcgtact gcccggggtg
     1621 cttcttgtag tagtcctggt ggtactcctc ggccgggtag aatcggcttg cgggctcgat
     1681 cggcgtgacg agcggccggt cgaaccgacc gctcgcgccg agctcttgct tggaccgctc
     1741 ggcgatctcg cgctgtttct cgtcgtgata gaagatcgcc gtgcggtatt gcgagccgcg
     1801 atcggcgaac tgtccgcccg cgtcggtcgg gtcgatcgag ctccagaaga cacggaccaa
     1861 cgtctcgtag tcgacgagcg ccgggtcgta gcgcacttgt accgcttcgg cgtggccggt
     1921 cgcgcccgag gagacttgct cgtagctcgg ctgcttctcc ttcccgccgg tatagcccga
     1981 gacgaccgag cgcacgccgg cgagcttctc gaacgggggc tccatgcacc agaagcagcc
     2041 gccggcaaac gtcgccagct cgagtccggc tccgccgtcg cccgcgtccg accccgagca
     2101 cgcgagtatc gatgacaaca ccgcgccggc gaccagcgtg gagaagacct tgcgagatcc
     2161 gtagctcatc tcgactcccc ggccgctctg cggccacgcc atggaaacag ccgtcccggg
     2221 cgggatgttc ccgcgggtcg ctgccgcgtg agggtcaaag gcggccgtag accgggacgg
     2281 cggccccggt gaccgatcgg gccgcctcgg aggcgagaaa gacggcgaca tccgccaccg
     2341 cctccggttc gacccacgtc gagggatcgg cgtcgggcat cgccgcgcgg ttctgcggcg
     2401 tatcgatcgt ccccggcacg acgcagttga cgttgacgcc cgcgcccttc acctcgtgag
     2461 ccaggctctc ggtcagccgc agcacggcgg atttcgacgc ggcgtaggcc gccaggcccg
     2521 ccggggcgcc gaaccccgcg cgagcggcgg tgttgacgat cctcccccgc ccgcgctcga
     2581 ccatcgacgg cacgaccgcc cggcagccgt tcaacgtggt gtcgaggttc atccgcagca
     2641 tgcgccgcca ggtgtcgggc gactcgtccc cggcgacctc cgccgcggcg aagccgccga
     2701 cggtgttgaa cagcgcgtcg atgcgaccga acgagcgcag cacgcggtcg accgccgcgg
     2761 ccacctcgtc gaaccgcgtc atgtcgacgc cctcgacaca gagcagctca ccaccgccgg
     2821 tgagatcgcc gaagatcccc ggcagccggc cctcgctgtg atcgacggcg acgacccgtg
     2881 ccccggcccg ccggaagcgg cggactccgg cctgacccag attgcccgcg gctcccgcga
     2941 tcatcaccac cctgtcggtc atgtcgaaca tggctcgatc gcctcctctc gtccgaaaac
     3001 cggcgttcgg ctggtaagat gatgcaaccg tgtcggagca agtcgcaacg gcaatttcgg
     3061 tcggcctcgc gctctacttc ggcgccggcg tgttgttcgc gatcccgttc gtcatccgcg
     3121 gcgtcgatcg catcgacgag gtcgcgcgcc acagcgggtg ggggtttcgg ctgatgatcc
     3181 tgcccggctc gatcgcgctg tggccgatcc tgcttcggcg ttggatcggc ggcgcgcagc
     3241 ccggcgagga gtcgaacgcg caccgcctgg cggcgaggaa gtcgcggtga tccgctcgtt
     3301 gcgccgacgg caccgcctgc tggtcgtggc gatcggcacc gccaccggtc tgctgctcgt
     3361 gctgggtctg ttgtcgcggc gtccgccgcc ccgcgtgccg tcgctcggcg aactggaccg
     3421 agccgaaagc gcgggcgagg ccgcgccggt cgtcgatcga tgagcgtcgc ctaccgcgcc
     3481 gtcctatgga accggcagaa gcgcatctac gacacggtga tcgcgatcgg cgtcgtctcg
     3541 tacctcgtct cgttcgtcgg tctgggcctc gccgtccggc cgcaggcgac cgtcgagacg
     3601 ttgatcatcc gcgccagcgg cacgctcggt ctcctgctgt tgcacatcat cctgtcgatc
     3661 ggcccgctgt gccggctcga tgctcgattc ctgccgctgc tgtacaaccg gcgtcacctg
     3721 ggcgtgacga tgttcctggt cgctgcgctg cacgcgacct tcgccctgtt ccagtttcac
     3781 gcgttgggcg acgtccaccc gctggtcagc ctgctgtcgt cgaacacgcg attcggcagc
     3841 tcgagccagt ttccgttcga gctgctcggc ctgggcgcgc tctcgatcct gtcgctgatg
     3901 gcggcgacaa gccacgattt ttggctgcgc aacctgtcgg cgccggtgtg gaagtcgctt
     3961 cacatgctgg tctacctggc ttacgccctg ctggtgctgc acgtcgtgct cggggtgtta
     4021 caggacgaaa ccgatccgct gctggtcggc gtgctcgtct tcggtttcgt ctgggtcgtc
     4081 gggctgcagg tcgcggcggg gttcggcgag cgccgcgcgg acggcgagct gtcggcggcg
     4141 ctggccgacg ggtacatgcc ggcgtgctct gtcgacgaga tccgcatcgg tcgcgcgcgc
     4201 gtcgtctgcc tcggcggcga acgggtggcg atcttccgct acgacgacaa gatctccgcg
     4261 gtgtcgaacg tctgtcagca ccagaacggg ccgctcggcg aaggtcggat cgtcgacggc
     4321 tgcatcacct gcccgtggca cggctaccag tacctccccg acagcggtgc ctccccgccg
     4381 ccgttcaacg agaagatccc gacgttcaac gtccgcgtcg ccggcggccg cgtgctggtc
     4441 gacccgcggc ccaacccgcc aggcacccgc gtcgaaccgg cgcgcgtcgg cggcggagcg
     4501 cgcggtggct gacggacgga gcgattttta catcggctac gagccccgcg gcccgcgtcg
     4561 gctcgcgcgc cgcatgcgcc tcgtgatcgc gttgcttttc gcgctggccc tcgcgctggc
     4621 ggcgctgttg gtcggcgcgc aacgttcgtt cgacggtggc gtgttcgagt tcggcgtgga
     4681 ccgccagctc gtcggcacgc tcgtcgagca accctacccg ctattgcgcg tccccggcga
     4741 cgacggcgcg ccggctcgca cccacctgct cgtcggccac ggcaagcacg gagcgggacc
     4801 gctcgtcgcc gggctcgacg gtcgggtcgt tcgcgtcagc ggctcgccga tccaccacgg
     4861 ccggcatcgc atgatcgaga tccaccgcgt cgagacgatc gacgcggtgg gcggcgcgat
     4921 gccgtccgag cgggcggctc tgggcacgat gacgctcgcc ggcgagatcg tcgattcgaa
     4981 atgccatctc ggcgtgatga aacccgggcg cggcaagacc caccgggcct gcgcggtgcg
     5041 ctgcatcagc ggcggctccc cgccgctgtt gcgcgtcgag gaccgcacgg ggcgtgtcga
     5101 gctgttcctg ctcgtcgacg aagctggggc gagcgtcaac cggcgcgtgc tgtcgatgg
//
LOCUS       DASPGN010000147         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_610537, whole genome shotgun sequence.
ACCESSION   DASPGN010000147 DASPGN010000000
VERSION     DASPGN010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610537"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..445
                     /locus_tag="VD788_04100"
     CDS             194..445
                     /locus_tag="VD788_04100"
                     /inference="COORDINATES: protein motif:HMM:NF016265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+)-trafficking protein"
                     /protein_id="HXV75478.1"
                     /translation="MGQVACSRCGSLAEGLARPPLPPPWGERLLEQTCAGCWDDWKET
                     QVKLINEYRLNVVDPADFARLMDEMSTFLALRGDGRDEV"
     gene            complement(465..644)
                     /locus_tag="VD788_04105"
     CDS             complement(465..644)
                     /locus_tag="VD788_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75479.1"
                     /translation="MSDARTTERPARVQLHLVLPYKFDPIHHPRVRGYLEQGYRIEQF
                     QRLTDQEALVTLARG"
     gene            846..1169
                     /gene="erpA"
                     /locus_tag="VD788_04110"
     CDS             846..1169
                     /gene="erpA"
                     /locus_tag="VD788_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HXV75480.1"
                     /translation="MLNLTDNAVKKVKNLLEAENKAGFGLRVAVQGGGCSGFQYGLTW
                     EKEEKPNDNVLEFAGLRVFVDPLSAMYLEDVRIDYVDSLSGSGFKIDNPRASGTCGCG
                     SSFSV"
     gene            1166..2308
                     /locus_tag="VD788_04115"
     CDS             1166..2308
                     /locus_tag="VD788_04115"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HXV75481.1"
                     /translation="MTGPLRTLAPYAQRSEHSRGRRHDQRGHEYRDPYQRDRDRIIHS
                     RAFRRLEYKTQVFVNHEGDHYRTRLTHTIEVSQIGRTVARALSLNEDLVESLALSHDL
                     GHTPFGHLGEQVLDRMLDDCGGFNHNRQTLRIVEQLEERYPQFPGLNLTWEVREGIAK
                     HSGPIDVARAPEFAEYEPELLPPLESQSIDLVDEIAYNHHDIDDGLESGLLSLDDLAD
                     AVPLFGRPLRQARVDHPTAPARLAYGVALRAMIDGLVSDLIEHTAGTLAESGVGSVGD
                     VREHSVALVGLSPTVTEENRQLKRYLHGHLYRHHRIERMKDKARRILVALFERYRDNP
                     LLLPDETRSRADRDGLARTIADYIAGMTDRYAIQEYQRLFDPGVRA"
     gene            complement(2312..>3779)
                     /locus_tag="VD788_04120"
     CDS             complement(2312..>3779)
                     /locus_tag="VD788_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXV75482.1"
                     /translation="AFERELADELLVGKWTNLVAQSVTADDADLEALFRQRNEKTSID
                     YFVVSAAEQDVATTVDEVDIEQWYEEHPEDYTREAGRRIRLVKIERQAAPEGIEIAPN
                     EIEAAYQANLARYSHGEQRRARHILFRAGADASDEQHQAARAQAAGVLDRLGAGEDFA
                     TLATELSEDTFSAQRGGDLDWFDRGDMVGPFDEAAFSMSPGDAPRLVETQFGYHVLEV
                     TDRREAGARPIDEVRDEIVSELRLARAEELMAINARRIRDRMDSADDFATVAEEEGLE
                     IASRFVNPREGLTDLRAPAEFRAAVLELEAGDISAPLRLPDALALVVVDELIPEGVAP
                     LEEVRNRVSTDVLNFRAKEAAVSAAQNAADLYESLAEMAESLGKEVRESGELGPDQAI
                     PTTGGGSAAVRAALFGEQTQVGDRGVVDVPAGALVFEITSRQPFDPTMFEIEKDALRT
                     ELVERKRLGMRRSLVDQLSQDLKIVVNDQLVERIDGGV"
BASE COUNT          597 a         1293 c         1258 g          631 t
ORIGIN      
        1 cttcccgagt gtgagagacc atcatctggt tcgagtagac cccgccgatc gcgtgtggcg
       61 ggaacttgat cggtagcttc tgctgcttgg ccatcggctc cctcgcgggc gccgcgtgtt
      121 tgcggcgtcg agatgcgtca tgatagtacg cacaggacgg ctcgggacaa cggcgagccg
      181 ggttggagtg agcatgggac aggtcgcgtg ttcgcgttgc gggtcgctcg ccgagggtct
      241 ggcccggccg ccgcttcccc cgccgtgggg cgagcgactg ctcgagcaga cctgcgccgg
      301 gtgttgggac gattggaagg agacgcaggt caaactgatc aacgagtacc gcctcaacgt
      361 cgtcgatccg gccgacttcg cgcggctgat ggacgagatg tcgacattcc tcgccctgcg
      421 cggcgatgga cgagacgagg tctgaccggc gacggccgcg tccgtcaacc gcgggccagg
      481 gtgacgagcg cctcctggtc ggtgagccgc tggaactgct cgatgcggta gccctgttcg
      541 agatagccgc gcacccgcgg gtggtggatc gggtcgaact tgtagggcag gacgagatgg
      601 agctggaccc gcgcgggacg ctctgtcgtc cgggcgtcgc tcatcgtttc tctccccggt
      661 tcggtcggga actcggcccg tcaccggccg cagaatagcg acctcgcggc acaggtgcaa
      721 ctcgttcggc cggcacggct tgccccggct tcgttgacag ccacggcgga ttcccctact
      781 ttctaacggg gaaggtcgcg acgtcgcgcg agacgtccac cgctttcccg gaggtcgtac
      841 ccgacatgct gaacttgacc gacaacgccg tgaagaaggt gaagaacttg ctcgaggccg
      901 agaacaaggc cggcttcggc ttgcgcgtcg ccgtccaggg cggcggatgc tccgggtttc
      961 agtacgggct gacctgggaa aaagaagaga agccgaacga caacgtgctc gaattcgccg
     1021 gcctcagggt tttcgtcgat ccgctgtcgg cgatgtacct cgaggacgtc cgaatcgact
     1081 acgtcgacag cctcagcggg tcggggttca agatcgacaa cccgagggcc agcggcacct
     1141 gcggttgcgg ttccagtttc tcggtgtgac cggaccgctc cgcacgctcg ctccctacgc
     1201 ccagaggtcc gagcactccc gcggcagacg ccacgaccaa cgcgggcacg agtaccgcga
     1261 cccgtatcag cgcgaccggg accggatcat ccactcgcgc gccttccggc gtctcgagta
     1321 caagacccag gtgttcgtca accacgaggg cgatcactac cgcacgcgcc tgacccacac
     1381 catcgaggtg agtcagatcg gtcgcacggt cgcacgggcg ctgtcgctca acgaggacct
     1441 cgtcgaatcg ctcgccctgt cacacgatct gggccacacg ccgttcggcc atctgggcga
     1501 gcaggtcctc gaccggatgc tggacgactg cggcgggttc aaccacaacc gccagacgct
     1561 gcgcatcgtc gagcagctgg aagaacgcta cccgcagttt cccggcctca acttgacgtg
     1621 ggaggtgcgc gagggcatcg ccaagcactc cggtccgatc gacgtcgcgc gggcgccgga
     1681 gttcgcggag tacgagcccg agctgctgcc cccgctcgaa tcgcagtcga tcgacctggt
     1741 cgacgagatc gcctacaacc accacgacat cgatgacggc ctcgagtcgg gcctgttgtc
     1801 gctcgacgat ctggccgacg ccgttccgct gttcgggcgg cccctgcgcc aggcgcgcgt
     1861 cgatcatccg accgcgcccg cccgactggc ctacggcgtg gcgttgcgcg cgatgatcga
     1921 tggtctcgtg tccgatctga tcgagcacac cgccggcacc ctcgccgaat cgggcgtcgg
     1981 ttcggtcggc gacgtgcgcg agcactcggt cgcgctggtc ggcctgtcgc cgacggtgac
     2041 agaggagaac cgacagctca agcgctatct ccacggtcat ctgtaccggc accaccgcat
     2101 cgagcgaatg aaagacaagg cgcggcggat cctggtcgcg ttgttcgagc ggtaccgcga
     2161 caacccgttg ttgctgcccg acgagacgcg ctcgcgcgcc gatcgcgacg ggctcgcccg
     2221 cacgatcgcc gactacatcg ccggaatgac ggaccgctac gcgatacagg aataccagcg
     2281 gctgttcgac cccggtgtca gggcctgacg gtcagacccc gccgtcgatc cgttcgacca
     2341 gttgatcgtt gaccacgatc ttgagatcct ggctcaactg gtcgaccagc gagcgacgca
     2401 tgcccaaccg cttgcgctcg accagctcgg tacgcaacgc atccttttcg atctcgaaca
     2461 tcgtcggatc gaacggctgc cggctggtga tctcgaagac caacgcgccg gccggcacgt
     2521 cgacgacacc gcggtcgccg acctgcgttt gttcgccgaa caacgcggca cgaaccgcgg
     2581 ccgagccgcc gccggtcgtc gggatcgcct ggtcgggacc cagctcgccg gactctcgca
     2641 cctctttgcc gagcgattcg gccatttcgg cgagcgactc gtagaggtcc gccgcgttct
     2701 gcgcggccga caccgcggcc tccttggcgc ggaagttgag cacgtccgtg ctgacccggt
     2761 tgcggacctc ttcgagcggc gcgacgccct cgggtatgag ctcgtcgacg acgacgaggg
     2821 ccagcgcgtc gggcagtcgc aacggcgcgc tgatgtcgcc cgcctccagc tcgagcaccg
     2881 ccgcgcggaa ctcggccggg gcgcgcaggt cggtcaggcc ttcgcgcggg ttgacgaagc
     2941 ggctggcgat ctcaagcccc tcctcttcgg cgacggtcgc gaagtcgtcg gccgagtcca
     3001 tccggtcgcg gatcctccgg gcgttgatcg ccatcagctc ttcggcgcgc gcgaggcgca
     3061 gctcggacac gatctcgtcg cgcacctcgt cgatcggcct cgcgccggcc tcgcgccggt
     3121 cggtgacctc gagcacgtgg tagccgaact gggtctcgac cagcctcggg gcgtcgccgg
     3181 gcgacatcga gaacgccgcc tcgtcgaacg gtccgaccat gtcgccccga tcgaaccagt
     3241 cgagatcgcc gccgcgctgc gccgaaaagg tgtcctcgga cagctccgtc gcgagggtgg
     3301 cgaagtcttc gccggcgccc aaccggtcga gcacccccgc cgcctgggcg cgtgccgcct
     3361 gatgctgctc gtccgacgcg tccgcgccgg cgcgaaacag gatgtggcgg gcgcgtcgct
     3421 gttcgccgtg cgagtaccgc gcgaggttgg cctgataggc ggcctcgatc tcgttcggcg
     3481 cgatctcgat cccctccggg gcggcctgac gctcgatctt gaccagacgg atgcgacggc
     3541 cggcctcgcg cgtgtagtcc tcagggtgct cttcgtacca ttgctcgatg tcgacctcgt
     3601 cgacggttgt cgcgacgtct tgctcggcgg ccgacacgac gaagtagtcg atcgaggtct
     3661 tctcgttgcg ctggcggaag agcgcctcga gatcggcatc gtcggcggtc accgattggg
     3721 cgaccaggtt cgtccacttg ccgacgagca actcgtcggc aagctcgcgc tcgaatgcg
//
LOCUS       DASPGN010000148         7817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_259594, whole genome shotgun sequence.
ACCESSION   DASPGN010000148 DASPGN010000000
VERSION     DASPGN010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259594"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1255
                     /gene="purM"
                     /locus_tag="VD788_04125"
     CDS             206..1255
                     /gene="purM"
                     /locus_tag="VD788_04125"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HXV75483.1"
                     /translation="MDDEQTRGMTYRDAGVDIDAQDEALRRIKQLVASTRTPGVLSDI
                     GAFGGVFAPSFAGFEQPLLVSSADGVGTKLQVAFRCGVHHTVGRDLVHHCINDILVQG
                     ARPLFFLDYIATGRLDPPVLAAVVEGVAGACREHGCALLGGETAEMPGFYADGEYDLA
                     GFIVGAVDRPKLLGAERVRVGDKLIGLASSGLHTNGYSLARKVFFEHQRLDVGDRVEQ
                     LGGTVGELLLAVHRCYLPALGLPLERGWVRALAHVTGGGLTDNVPRSLPVGCGAEIRR
                     GSWPVPPIFGWLADRGKIAIEEMYRAFNMGLGMVLIASPDDCPRLERHFDDLREPHFD
                     IGRVVDRGSGVSYVD"
     gene            1245..1868
                     /gene="purN"
                     /locus_tag="VD788_04130"
     CDS             1245..1868
                     /gene="purN"
                     /locus_tag="VD788_04130"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXV75484.1"
                     /translation="MSTSGRVGILISGRGSNMVALIEAMQRGEIAAETAIVISNRPDA
                     GGLARARALGVMTRVVDSGVVKPRKLHEQRVIELLREQRVDLVCLAGYMRLLSAGMVA
                     EFRQRILNIHPSLLPAFPGLDAQRQALDYGVRFTGCTVHFVDEACDHGPIVLQAAVPV
                     EPDDDEARLSARILEQEHRLYVRAVRLFFDQRLEIDGRRVTIAGGVS"
     gene            1887..2615
                     /gene="radC"
                     /locus_tag="VD788_04135"
     CDS             1887..2615
                     /gene="radC"
                     /locus_tag="VD788_04135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXV75485.1"
                     /translation="MVRESTGPWPAVPADEPDCEPGGGGALDLPRALRPRQKLFARGP
                     RALSPSELVALVLGGGTRSEQVARRLLRRHGLAGLSRMGPTAWRGEVGLGVVAAARMT
                     AAFELGRLAHGRSDDDRPRVGSPGEAFRQVKSLGRARKEHLVGLYLDAQNALLSRETI
                     SIGSLNTTRTHPREVLYPAIRHLALGFILAHNHPSGCLDPSAQDVEFTRSIRQAAELM
                     GFELYDHLVVTRSGYTSFRERGLL"
     gene            2683..3918
                     /gene="tyrS"
                     /locus_tag="VD788_04140"
     CDS             2683..3918
                     /gene="tyrS"
                     /locus_tag="VD788_04140"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXV75486.1"
                     /translation="MEPSRFRPVDEQFDILSRGVVDLVTPDELRRKLERSNADRRPLV
                     VKVGFDPTAPDIHLGHTVLLRKMRQFQDLGHRVIFLIGDFTGMIGDPSGRSATRPPLS
                     REQILANAETYKRQCYKVLDPERTEIRFNHEWLGQLDAEGFIRLAAQYNVARMLERND
                     FKKRYQSGQSIALHEFLYPLAQAYDSVALEADVELGGTDQLFNLNVGRDIMPGYGLEP
                     QVVLTTPLIEGLDGVEKMSKSLGNYVGVEEPPRQIYGKLMSISDELMWRYYEHCTDVS
                     PETVRQMRQLVENGRLHPKQAKEGLALRVVTELYDESSAKAAREEFERVFRERGVPDE
                     IPRHRFAAEDGGVFLPRLLTEAGLAASRSEANRLLRQGAVSVDGQRLPADALRLDVTA
                     GDELLVKVGKRRFARVTIV"
     gene            complement(3915..4292)
                     /locus_tag="VD788_04145"
     CDS             complement(3915..4292)
                     /locus_tag="VD788_04145"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HXV75487.1"
                     /translation="MKRRVERRVHGDVQGDRVRAYDEVWAVVRRIPRGRVMTYGQIAT
                     WIENRLSARAVGWALHGCPDGVPWQRVVNATGSCSTDRLPDIPEGLQRAMLEAEGVVF
                     GENGVLDLRRYRYVPRPPRRRSR"
     gene            4434..4886
                     /locus_tag="VD788_04150"
     CDS             4434..4886
                     /locus_tag="VD788_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXV75488.1"
                     /translation="MYDIRTILLTTDFSDTSRRALDPARAVARRFGARIVLVYVEEDR
                     LPPLVVEYMAVGVEDVLNQQVEQSRKRLEEFAADHLAGIDGLELVVGVGTPHVEIVRL
                     AQEHAADLIVMATHGRGVISHALMGSTTERVLRRAPCPVLVACSDRAD"
     gene            complement(4889..6946)
                     /locus_tag="VD788_04155"
     CDS             complement(4889..6946)
                     /locus_tag="VD788_04155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HXV75489.1"
                     /translation="MIAAVRQIARRSTPQAALFALLAAATPATASVSIYVTPEALAER
                     APWIVEATVVAGGTGYDDETGQLATYVTLDVEHVLRGRLPGPTIVLRELGGQFGGWAH
                     ETDASPHYQIGERVVAFLELADDGSLRTSGLFFGKYAVDGDGGWAERDLAGRGTIRHR
                     PLDAVERMPLADLRSLVASTALAPEPASARQPAHSRPTRSRAGAPALPLATPPEFGRL
                     RWGADRRSVEIGSRTLDRQDGSYADALPGLETAAVPVAPRFASADASAPARWAEIDQG
                     GTLLVDIDPRDNPLGDDDAAVLQIERAMSAWTDVPESRVTIKTGNPSLEFGAASPALA
                     YSGTNVVLFDDPFSNISDPSPNCSGVLAIGGYWRTSTPTSTVHGIAFRGAAQLYVIFN
                     NDFECVLSEPNDLAEIATHELGHGIGLGHSSVPDAIMRSSAYRFRGPRLGDDDADAAH
                     CFYPHQLTLNAPDGGESWVSGAIEEIRWGSTLELGPDDGVVDLEYSIDGGASWIAIDG
                     GQPNDGSYFWEVPAVETDEARVRVSRSSRTGSSSPPFPSRCSGDASDGSFEITIQLPS
                     AGSVPRGSADDGGLTVERNGWGGLDLDWGASCSPDAEDYAVYSGSLDALRQGVWDHAP
                     IVCSTGGLTTAETTLGSGNLYFLVAPLAAAGEGRLGRGAGATWRPASASACAEREKVE
                     ACE"
     gene            7151..>7817
                     /locus_tag="VD788_04160"
     CDS             7151..>7817
                     /locus_tag="VD788_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXV75490.1"
                     /translation="MTHGWAPSTLEWLECSRCRTALDWRLPQGLCPKCGGVLLARYRL
                     EQARRSMTRAALAGRPPGLWRFAEVLPRIGEPLTLGEGATPLHHARFLGQSIGFDRLF
                     IKDESLNPTGSFKARGMATALTMARDLGVRRVALPSAGNAGGAAAAYAALAGLAVELF
                     LPGETPEPFRLEAAAYGATVHLVDGDIADCGVRVREGVEREGWFDLSTLREPYRLEGK
                     KTMG"
BASE COUNT         1163 a         2637 c         2825 g         1192 t
ORIGIN      
        1 cgaggtgtgt accgcgtgct ggagcgacga ccagccggtc gcgctgccga tcggcgaagg
       61 tcggcagctc cggctgttcg acaagacgca acgatgagcc gcgccgacgg acggtggttt
      121 ccccccggca accggctggc tggacccgcc ggcatcgagg cacgagaatt gggttctacg
      181 cgaccgtaga gcgtgcgagg cggggatgga cgacgaacag acgcgaggca tgacctatcg
      241 ggacgcggga gtcgacatcg acgcccagga cgaggcgttg cgccgcatca agcagctcgt
      301 cgcctcgacg cgcacgcctg gtgtgctgag cgacatcgga gcgttcggcg gcgtcttcgc
      361 cccgtccttc gccggtttcg agcaaccgct gctggtctcg agcgccgatg gggtgggcac
      421 gaagctgcag gtcgcgttcc gctgcggtgt ccaccacacg gtcggtcgcg acctggtcca
      481 ccactgcatc aacgacatcc tggtccaggg agcgcggccg ctgttcttcc tggactacat
      541 cgcgacgggt cggctcgacc cgcccgtgct ggccgcggtc gtcgagggcg ttgccggcgc
      601 gtgccgcgag cacggctgcg cgttgctcgg cggcgagacc gcggagatgc ccgggttcta
      661 cgccgacggc gagtacgatc tggccggctt catcgtcggc gcggtcgatc gaccgaaact
      721 gctcggcgcg gagcgtgtcc gtgtcggtga caagctgatc ggtctggcgt cgagcgggct
      781 gcacaccaac ggctactcgc tggcgcgcaa ggtgttcttc gagcaccaac ggctcgacgt
      841 gggcgatcgc gtggagcagc tcggcgggac ggtcggtgag ctgctgctgg cggtccaccg
      901 ctgctacctg ccggcgctcg gcctcccgct cgagcgcggt tgggtccgtg cgctggcgca
      961 cgtgacgggc ggcggcttga ccgacaacgt gccgcgatcg ctaccggtag gctgcggcgc
     1021 cgagatccgt cgcgggagct ggccggtgcc gccgatcttc ggctggttgg ccgatcgcgg
     1081 gaaaatcgcc atcgaggaga tgtatcgcgc gttcaacatg gggctcggca tggtgctgat
     1141 cgcgtcgccc gacgattgcc cgcggttaga gcggcacttc gacgacctcc gcgagccgca
     1201 cttcgacatc ggtcgggtcg tcgaccgcgg gtcgggcgtg agctatgtcg actagcggtc
     1261 gtgtcggcat cctgatatcg ggccgcggca gcaacatggt ggcgttgatc gaggcgatgc
     1321 agcgtggcga gatcgcggcg gagacggcga tcgtcatctc caaccggccc gacgccggcg
     1381 gtctcgcacg ggcgcgtgcg ctcggcgtaa tgacgcgggt cgtcgacagc ggcgtggtca
     1441 agccgcgcaa gctgcacgag cagcgggtga tcgagctgct gcgcgagcaa cgagtcgatc
     1501 tggtctgttt ggcgggttac atgcgcctgc tcagtgccgg gatggtggcc gagttccgcc
     1561 agcggatcct caacatccac ccgtcgttgc tgcccgcgtt ccccgggctc gacgcccagc
     1621 gccaggcgct ggactacggt gtgcgattca ccggctgcac cgtgcacttc gtcgacgagg
     1681 catgcgatca cgggccgatc gtgctgcagg ccgcggtccc ggtcgagccc gacgatgacg
     1741 aggcgaggtt gtcggcgagg atcctcgaac aggagcaccg cttgtatgtc cgcgcggtgc
     1801 gtctgttctt cgaccagcgt ctcgagatcg acggccggcg agtgacgatc gccggcggcg
     1861 tgtcctgagc ctccgggagt cgaatcgtgg ttcgagagtc gaccgggccg tggcccgccg
     1921 tgccggccga cgagccggac tgcgagcccg gggggggcgg agccctcgat ctcccgcgcg
     1981 cgctgcggcc gcgccagaag ctgttcgcgc gtggtccgcg cgcgctgtcg ccgagcgagc
     2041 tggtggcgct ggtgctcggc ggtggcacgc ggagcgagca ggtcgcgcgc cggttgttgc
     2101 gccgccacgg tctggccggg ttgtcccgga tgggccccac ggcctggcgc ggcgaggttg
     2161 gcctcggcgt cgtcgccgcg gcgcggatga cggcggcatt cgagctcgga cggctggcgc
     2221 acggcaggtc cgacgacgat cgtccgcggg tcggcagccc gggagaggca ttccgccagg
     2281 tcaagtcgct tggtcgcgcg cgcaaggagc atctcgtcgg gctgtacctc gacgcgcaaa
     2341 acgcgttgtt gtcgcgcgag acgatctcga tcgggtcgct gaacacgacg cggacgcatc
     2401 cgcgggaggt gctgtatccc gcgatccgtc acctcgctct cggcttcatc ctggcccaca
     2461 atcatccgag cggatgcctc gacccatcgg cacaggacgt cgagttcacg cggtcgatcc
     2521 gtcaagcagc cgagctgatg ggtttcgagc tctacgacca cctcgtcgtg acccgctcgg
     2581 gttacacgag ctttcgcgag cgcggactgc tgtaggcggc ggcgcgccgc cgtctgctag
     2641 aatcgcggct tcgcgatcca gcgccagcga gggactgccg cgatggagcc gagccgattc
     2701 cgaccggtcg acgagcagtt cgacatcctg tcgcgggggg tcgtcgacct cgtcacaccc
     2761 gacgagcttc gacgcaagct cgagcgatcg aacgccgacc ggcggccgct cgtggtcaaa
     2821 gtcggcttcg acccgacggc gcccgacatc catctcggac acacggtgct gctacgcaag
     2881 atgcggcagt ttcaggacct cggccaccgc gtcatcttcc tcatcggcga cttcaccggt
     2941 atgatcggtg atccgtcggg gcgttcggcg acccgcccgc cgctgtcgcg tgagcagatc
     3001 ctggccaacg ccgaaaccta caagcgccag tgctacaagg tgctcgaccc ggagcggacg
     3061 gagatccgtt tcaaccacga gtggttgggg cagctcgacg cggagggctt catccggctc
     3121 gccgcgcaat acaacgtcgc ccgcatgctc gaacggaacg atttcaagaa gcgctaccag
     3181 agcggccaat cgatcgcgct acacgagttc ctctacccac tggcacaggc ctacgactcg
     3241 gtggcgctcg aggccgacgt cgagctcggc gggacggacc agttgttcaa cctcaacgtc
     3301 ggacgcgaca tcatgccggg ctacgggctc gagccgcagg tcgtgctgac gacgccgctg
     3361 atcgaaggcc tggacggcgt cgagaagatg tcgaagtcgc tcggcaacta cgtcggcgtc
     3421 gaagaacccc cgcggcagat ctacggcaag ctgatgtcga tctcggacga gctgatgtgg
     3481 cgctactacg agcactgcac cgacgtgtcg ccggagaccg tgcggcagat gcggcagctg
     3541 gtagaaaacg gtcggctcca ccccaagcag gcgaaggagg ggttggcgct ccgggtggtg
     3601 accgagctgt acgacgagtc ctcggcgaag gccgcgcgcg aggaattcga gcgcgtcttt
     3661 cgcgagcgcg gcgtgccgga cgagatcccg cgccaccgct tcgcggccga ggacggcggg
     3721 gtcttcctgc cgcggctgtt gaccgaagcc gggttggccg cgtcgcgctc ggaggcgaac
     3781 cgattgctcc ggcaaggcgc cgtgtcggtc gacggccagc gactgccggc agacgcgctt
     3841 cgcctcgacg tgacggccgg cgacgagctg ctggtcaagg tcggcaagcg gcggttcgcg
     3901 cgcgtgacga tcgtctagcg tgagcgccgg cgaggcgggc gcggaacgta gcggtagcgt
     3961 ctcaggtcga gcacgccgtt ctcgccgaag acgacaccct ccgcctcgag catcgctcgt
     4021 tgcaacccct ccggaatgtc gggcagccgg tcggtcgagc agctgcccgt ggcgttgacg
     4081 acccgttgcc acgggacccc gtccgggcat ccgtggagcg cccagccgac cgctcgagcg
     4141 gacagccggt tctcgatcca cgtggcgatc tggccgtagg tcatcacccg gccgcgcggg
     4201 atgcgacgca ccaccgccca gacctcgtcg taggcccgaa cgcggtcgcc ctgcacgtcg
     4261 ccgtggaccc ggcgttcgac ccgcctcttc atcggcgctc ctccgctcgc gatgttactc
     4321 cgaccccgac tcggaaccga gcccgatcgg cactatcatg gaccatagcc gcggtcgtga
     4381 cggacctcgg gcgtacggca tcgtcgcgcc gcggccggat ggaggaacgc acgatgtacg
     4441 acatccggac gatcctgctg accaccgact tctccgacac ctccagacgg gcgctcgatc
     4501 cggcgcgcgc cgtggcccgg cgattcgggg cacggatcgt gctggtctac gtcgaggagg
     4561 atcggctgcc gccgttggtc gtcgagtaca tggcggtcgg cgtcgaggac gtgctgaacc
     4621 agcaggtcga gcagtcgcga aagcggctcg aggagttcgc cgccgaccac ctcgcgggca
     4681 tcgacgggct cgagctggtg gtcggtgtcg ggacacccca cgtcgagatc gtacgtttgg
     4741 cgcaggagca cgccgccgat ctgatcgtga tggcgacgca cggccgcggc gtgatctcac
     4801 acgcgttgat ggggtcgacc acggaacgcg tgctgcgccg cgccccgtgt ccggtgttgg
     4861 tcgcctgctc cgaccgcgcc gactgaggct actcgcaggc ctcgaccttc tcgcgctcgg
     4921 cgcaggccga ggccgaggcg ggacgccacg tcgcgccggc cccgcgtccg aggcgaccct
     4981 cgccggcggc ggccaacggt gcgacgagga agtagaggtt gccggagccg agcgtcgtct
     5041 cggccgtcgt cagcccgccg gtgctgcaga cgatcggcgc gtgatcccac acgccttggc
     5101 gtaacgcgtc gagcgaaccg ctgtagaccg cgtagtcctc ggcgtcgggc gaacacgagg
     5161 caccccagtc gagatcgagc ccgccccagc cgttgcgttc gacggtcaaa ccgccgtcgt
     5221 cggcgctccc gcgcggaacg gaaccggccg acggcagttg aatcgtgatc tcgaacgagc
     5281 cgtccgaggc gtccccgctg caacgcgacg ggaacggcgg cgagctcgag ccggtgcggc
     5341 tcgagcggga aacgcgcacg cgggcctcgt cggtctcgac cgccggaacc tcccagaagt
     5401 agctgccgtc attcggttgc ccgccgtcga tcgcgatcca gctcgcgccg ccgtcgatcg
     5461 agtactcgag gtcgacgacg ccgtcgtccg gacccagctc gagcgtcgac ccccagcgga
     5521 tctcctcgat cgcgcccgag acccacgatt cgccgccgtc gggcgcgttg agcgtgagtt
     5581 ggtgcggata gaagcagtgc gccgcgtcgg cgtcgtcgtc accgagccgc ggaccgcgaa
     5641 agcggtatgc cgacgagcgc atgatcgcat ccgggaccga cgagtgaccg agaccgatgc
     5701 catgtccgag ttcgtgggtc gcgatctcgg ccaggtcgtt cggttcactg agcacgcact
     5761 cgaagtcgtt gttgaagatg acgtagagct gggccgcgcc gcggaaggcg atcccgtgaa
     5821 ccgtcgaggt gggggtcgac gtgcgccagt agccgccgat cgcgagcacg ccggagcagt
     5881 tgggcgaggg gtcgctgatg ttcgagaacg gatcgtcgaa cagcacgacg ttggtgccgc
     5941 tgtaggccag cgccgggctc gccgcgccga actcgagcga cgggttgccg gtcttgatcg
     6001 tcacgcggga ttccggcacg tcggtccacg cggacatcgc gcgctcgatt tggagcaccg
     6061 cggcgtcgtc gtctcccagc gggttgtcgc gtggatcgat gtcgacgagg agcgtgccgc
     6121 cttggtcgat ctcggcccag cgcgcgggcg ccgacgcatc ggcggaggcg aaccgcggcg
     6181 ccacgggcac tgccgccgtc tcgagtccgg gcagcgcgtc ggcgtacgag ccgtcttgcc
     6241 gatcgagcgt ccgcgagccg atctcgaccg atcggcggtc cgcgccccag cgcagccgac
     6301 cgaactcggg cggcgtggcg agcggaagcg cgggcgcgcc ggcgcgggag cgcgtcggtc
     6361 gggaatgcgc cggctgccga gccgaagccg gttccggggc gagcgcggtg ctcgcgacga
     6421 gcgaccgcag atcggcgagc ggcatccgct cgacggcgtc gagcgggcgg tgccgaatcg
     6481 tcccgcgacc cgcgaggtcg cgctcggccc aaccgccgtc gccgtcgacc gcgtacttac
     6541 cgaagaacag accgctggtc cgcagcgatc cgtcgtcggc caactcgagg aaggcgacga
     6601 cgcgctcgcc gatctggtag tgcggcgagg cgtcggtctc gtgggcccat ccgccgaact
     6661 gcccgcccaa ctcgcgcagc acgatcgtcg gcccgggcag ccggccgcgc agcacgtgct
     6721 cgacgtcgag ggtgacgtag gtcgcgagtt gcccggtctc gtcgtcgtag cccgttccgc
     6781 cggcgacgac cgtcgcctcg acgatccacg gagcccgctc ggcgagcgct tcgggcgtga
     6841 cgtagatcga cacgctggcg gtcgcgggcg tcgcagcggc gagcagcgcg aacagggccg
     6901 cctgaggggt cgaccgacga gcgatctggc ggacggcagc gatcattcca tgcctccacc
     6961 gttgatcgta agttgttgaa aacaagtatc aacgacgaat gtcgtaaggt cgctcgcccg
     7021 ccacgccccg atcgccgaaa aggccgaccg gcgtccgatc gaacgacggc cgtgcaaccg
     7081 gttgccacga gcgggcgcgc tcggctaccg tggccggcgc tcgcgcgcct tccggcggag
     7141 agggatgggc atgacgcacg gctgggcacc ttcgacgctc gagtggctcg agtgctcacg
     7201 ctgtcgaacg gcgctcgatt ggcgcctacc gcaggggctg tgcccgaagt gcggcggcgt
     7261 gctgctcgcc cgctaccggt tggagcaggc gcggcggagc atgacgcgag ccgcgctcgc
     7321 cgggcgcccg cccgggctgt ggcgcttcgc cgaggtcctg ccccggatcg gcgagccgct
     7381 gacgctcggc gaaggcgcga ccccgctgca ccacgcgcgt ttcctcggtc aatcgatcgg
     7441 gttcgaccgc ctcttcatca aggacgagtc gttgaacccg acgggatcgt tcaaggcgcg
     7501 cgggatggcg acggcgctga cgatggcgcg ggacctcggc gtgcgccgtg tcgcgcttcc
     7561 gtcggcggga aatgccggcg gagcggcggc ggcctacgcc gcgctcgccg ggttggccgt
     7621 cgagctgttc ctgccgggcg agacgccgga accgttccgt ctggaggccg cggcgtacgg
     7681 cgccacggtc cacctcgtcg acggcgacat cgccgattgc ggcgtccgcg tacgcgaggg
     7741 ggtcgagcgc gagggctggt tcgatctgtc gacgttgcgc gagccgtacc ggctcgaggg
     7801 caagaagacg atgggct
//
LOCUS       DASPGN010000149         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_610739, whole genome shotgun sequence.
ACCESSION   DASPGN010000149 DASPGN010000000
VERSION     DASPGN010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610739"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VD788_04165"
     CDS             <1..236
                     /locus_tag="VD788_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV75491.1"
                     /translation="FVMLPLWIMSGTFFSPARFPDGLQPAVQLLPLTAVNDALREVMI
                     DGAGLFEIAGELGIVCGWGLAALTVALALFRWH"
     gene            complement(192..>2048)
                     /locus_tag="VD788_04170"
     CDS             complement(192..>2048)
                     /locus_tag="VD788_04170"
                     /inference="COORDINATES: protein motif:HMM:NF014961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HXV75492.1"
                     /translation="RRPDATERLVEFADTVRGQRSSPKQEAEWRELPLAERLAHALVH
                     GIVTHVDEDVAEALASYPDPLSIIEGPLMDGMNVVGERFGAGKMFLPQVVKSARVMKK
                     AVAILEPLMRDAISAAPSARARIVMATVKGDVHDIGKNIVGAVLACNNYEIVDLGVMV
                     PADKIIETARRERADLIGLSGLITPSLDEMVHVAAEMQRQSLQLPLLIGGATTSAKHT
                     AIKIAPAYSGLTVHVTDASRAVDVVGQLSGKAPRDGFAEEVRRKQDQVRRRHDEGEPQ
                     PLRSYAEAQAARASLDRPAGEIDQPEFVGHRTLAPYPLGELIRYIDWTPFFHVWELRG
                     VFPRILDDDKYGESATELFHTARQTLDRLVDQRRLQAAGVYGFYPANRDGDDIIVWTD
                     AQRTAERLRFHTLRQQRSHDGARPSLALADFVADRSGPCDFVGAFAVTAGIGLDGLVA
                     EAERDHDDYSVIMLKALADRLAEAFAERLHEIARGDWGYGKDERLSPDELLRERFRGI
                     RPAPGYPACPDHSEKRTLWRLLDVERETGIRLTESYAMQPAAAVCGLYFAHPAARYFS
                     VGPIGRDQVASYAHRKGWTVADAERWLRPNLAYEPSDRSMPAEQGQGDRQGG"
BASE COUNT          298 a          719 c          682 g          349 t
ORIGIN      
        1 acttcgtcat gctgccgttg tggataatgt ccggcacctt cttctcgccc gcgcgctttc
       61 ccgacgggtt gcagccggcg gtccaactgc tgccgttgac ggccgtcaac gacgcgctgc
      121 gcgaggtgat gatcgacggg gccggcctgt tcgagatcgc cggcgagctg gggatcgtct
      181 gcggctgggg tctagccgcc ctgacggtcg ccctggccct gttccgctgg cattgaccgg
      241 tcggacggct cgtaggccag attcggccgg agccaacgct cggcatccgc gacggtccat
      301 cccttgcgat gggcgtagct cgcgacctgg tcccggccga tcgggccgac gctgaagtag
      361 cgtgctgcgg gatgggcgaa atacaacccg cagaccgctg ccgccggctg catcgcgtaa
      421 ctctcggtca accggatgcc cgtctcccgc tcgacgtcca gcagccgcca gagggtccgc
      481 ttctccgagt ggtcgggaca ggccggatag cccggcgccg gtcggatccc gcggaaacgc
      541 tcgcgcaaca gctcgtcggg cgacagccgc tcgtccttgc cgtaacccca gtccccgcgg
      601 gcgatctcgt gcagccgctc ggcgaaggcc tcggccaggc gatcggccag cgctttcagc
      661 atgatcaccg agtagtcgtc gtggtcccgc tcggcctccg cgaccaatcc gtcgagcccg
      721 ataccggccg tcacggcaaa ggcgccgacg aaatcacacg gtcccgatcg gtcggcaacg
      781 aaatcggcaa gcgcgagcga cgggcgtgcc ccgtcgtggc tccgctgttg ccgcagcgtg
      841 tggaatcgga gacgttcggc cgtacgctgc gcgtcggtcc agacgatgat gtcatcgcca
      901 tcgcgattcg ccgggtagaa gccgtagaca ccggccgctt gcaaccggcg ctggtcgacc
      961 aggcgatcca gggtttgtcg tgccgtgtga aacaactcgg tcgcgctctc gccgtatttg
     1021 tcgtcgtcga ggatccgcgg gaacactccg cgcaactccc aaacgtggaa gaacggcgtc
     1081 cagtcgatgt agcggatcag ctccccgagc gggtacggcg ccagcgtccg gtggccgacg
     1141 aattcgggct ggtcgatctc gccggcgggg cggtcgagcg acgcacgcgc cgcctgcgcc
     1201 tcggcgtagg agcggagcgg ttgcggctcg ccctcgtcgt gacgccgccg aacctggtcc
     1261 tgcttgcgcc gcacctcctc ggcgaagccg tctcgcggtg ctttgccgga gagttggccg
     1321 accacgtcga ccgcacgcga cgcgtcggtg acgtggaccg tcagccccga gtaggccggc
     1381 gcaatcttga tcgcggtgtg cttggcgctg gtcgtcgccc cgccgatcaa cagcggcaac
     1441 tggagcgact ggcgctgcat ctcggccgcc acgtgcacca tctcgtccaa cgagggcgtg
     1501 atcagaccgc ttagcccgat caggtcggca cgctcgcgcc gcgcggtctc gatgatcttg
     1561 tcggccggaa ccatcacgcc gaggtcgaca atctcgtagt tgttgcaggc cagcaccgca
     1621 ccgacgatgt tcttgccgat gtcgtgaacg tcgcccttga ccgtcgccat gacgatccgt
     1681 gcacgggcgc tcggcgccgc ggagatcgcg tcgcgcatca acggttcgag gatcgccacg
     1741 gccttcttca tcacgcgggc gctcttgacg acctgcggca ggaacatctt gcccgctccg
     1801 aacctctcgc cgacgacgtt catcccgtcc atcagcggcc cttcgatgat gctcaacggg
     1861 tcgggatagc tcgcgagcgc ctcggcgacg tcctcgtcga cgtgggtcac gatgccgtgc
     1921 acgagcgcgt gcgcgagccg ctcggcgagc ggcagctccc gccactcggc ctcctgcttg
     1981 gggctcgagc gttggccgcg cacggtgtcg gcgaactcga ccaaccgctc ggtcgcgtcc
     2041 ggtcgccg
//
LOCUS       DASPGN010000150         2567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_844855, whole genome shotgun sequence.
ACCESSION   DASPGN010000150 DASPGN010000000
VERSION     DASPGN010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844855"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VD788_04175"
     CDS             complement(<1..445)
                     /locus_tag="VD788_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXV75493.1"
                     /translation="MSDRLALVTGAAGEMGHLLIPALAARGFGVVTVDRRPLSDELAA
                     RCLRRHDASILDDGAMRALFAAHSFSHVYHLAAILSSKAERDPWQAHRVNVDGTFGLF
                     RLCQQVAAATGVSPTVVFPSSIAVYGLPDAETKRRAGAVRESQWLA"
     gene            519..1133
                     /locus_tag="VD788_04180"
     CDS             519..1133
                     /locus_tag="VD788_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV75494.1"
                     /translation="MSEPCRSTREIATIVPGVRHWSVLDDRVGFRSEAYAIDGPFGRV
                     LIDPLPFDDPVGNELEPISAICITGAFHQRSAWRLRRRFRVPVHAPHGAAPLVEPPDR
                     HYRDGEQPLAGLYARFRHGPTNPHAVLVWQAPSHRVLFSGDLLIRAAGGPFSLVPDEH
                     QQDPARTRAAVRALLDEAADVLCPAHGAPLTGGVAAAIAAAIAR"
     gene            complement(1139..1212)
                     /locus_tag="VD788_04185"
     tRNA            complement(1139..1212)
                     /locus_tag="VD788_04185"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1178..1180),aa:Gln,seq:ctg)
     gene            complement(1259..>2567)
                     /gene="gltX"
                     /locus_tag="VD788_04190"
     CDS             complement(1259..>2567)
                     /gene="gltX"
                     /locus_tag="VD788_04190"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXV75495.1"
                     /translation="SSDAMERAIVDGIEWLGLDVDEGPFFQSAARARHAGDAARLLAS
                     GHAYRCFCPPELLERTRRTAPGGGRDYVYPRTCRGLPAARSRQRTDAGEPFAVRFAVP
                     DREIGWRDLVHGDKRFAGDAIEDFILLRSDGSPTYMMSVVSDDVEMRISHVIRGDDHI
                     SNTPKQILLYEALERPTPHFAHLPLILGSDRKRLSKRHGAVSLLEYRERGYLPQAVLN
                     FLALLGWSPGDDRQKMTRDELVHSFDLGGVGRAGAIFDSEKLDWLNGQYLNELDAADI
                     AALVRTRLGAAGLWRDSFDAGEGELLAGTLELLKTRARTLDDFVERARPYLDPSDDFP
                     YEAQAADKHLGDEAVGERVARLAGRWSGLADWKAGLLEASLREVAHELGVSAGKLIHP
                     VRLAVTGHGVSPGIFEVLELLGRERSLRRLRRLIERVDRNAVS"
BASE COUNT          397 a          910 c          884 g          376 t
ORIGIN      
        1 gcgcgagcca ttgcgattct cggaccgcgc cggcccgccg cttggtctcg gcatccggca
       61 gcccgtagac cgcgatgctg ctgggaaaga cgacggtcgg ggagacgccg gtcgcggccg
      121 cgacctgctg acacagtcgg aacaggccga aggtgccgtc gacgttgacg cgatgggcct
      181 gccacgggtc ccgctccgcc ttgctcgaca ggatcgcggc caggtggtag acgtgtgaga
      241 acgagtgcgc ggcgaacagc gcccgcatcg cgccgtcgtc gaggatgctg gcgtcgtggc
      301 ggcgcaggca acgagcggcc aactcgtccg acaacgggcg tcggtcgacg gtcaccacgc
      361 cgaagccgcg tgccgccagt gccgggatca gcaggtggcc catctctccg gccgcgccgg
      421 tcaccagggc aagacgatcg ctcatgcagc acctcgttcg gggcaaccct gccggattcg
      481 tctcagcata tcaaaccgca gcggttatgc ttgtggccgt gagcgagccc tgccgatcga
      541 ctcgcgagat cgccacgatc gttcccggtg tccggcactg gtcggtcctc gacgaccgcg
      601 tcggcttccg cagcgaggcc tacgcgatcg acgggccgtt cggccgggtg ttgatcgacc
      661 cgctgccgtt cgacgatccc gtcggcaacg agctcgaacc gatcagcgcg atctgcatca
      721 ccggggcctt ccaccaacgc tcggcctggc gcctcaggcg ccggttccgc gtcccggttc
      781 acgcgccgca cggcgcggcg ccgttggtcg agccgccgga tcgccactat cgggacgggg
      841 agcaaccgct ggcgggactg tacgcccgct tccgccacgg accgaccaac ccgcacgcgg
      901 tgctcgtctg gcaggccccg tcgcatcgcg tgctgttctc cggcgacctg ttgatccgcg
      961 ccgccggcgg gccgttctcg ctcgtgcccg acgagcatca gcaggacccg gcacgcacgc
     1021 gagccgcggt gcgcgcgctg ctcgacgaag cggccgacgt gctgtgcccc gcgcacggcg
     1081 caccgctgac cggtggagtg gcggccgcga tcgcggccgc gatcgcgcgc tagcgcgatg
     1141 gctggggagc agggattcga accccgattc tacggtccag agccgcatgt cctaccattg
     1201 gacgactccc caggatcggt cgaacgaacc gaaaattata gcaaccgccc gatgcgggtc
     1261 aagaaaccgc gttccggtcg acacgctcga tcagccgtcg cagtcgacgc agggatcgct
     1321 cgcggccgag tagctcgagc acttcgaaga tacccgggct gacaccgtga ccggtgaccg
     1381 ccagccgcac ggggtggatc agcttgccgg ccgagacgcc cagctcgtgc gcaacctcgc
     1441 gcaggctcgc ctcgagcagt cccgccttcc agtcggcgag ccccgaccac cgacccgcga
     1501 ggcgcgccac ccgctcgccg accgcttcgt cgccgaggtg tttgtcggcg gcctgtgcct
     1561 cgtagggaaa atcgtcggac gggtcgagat acggccgggc gcgctcgacg aagtcgtcga
     1621 gcgtacgcgc gcgcgtcttg agcagctcga gcgtgccggc gagcagctcg ccctcgccgg
     1681 cgtcgaacga gtcgcgccac agcccggccg cgccgagccg cgtccggacc agcgccgcga
     1741 tgtcggcggc gtcgagctcg ttgaggtatt ggccgttcaa ccagtcgagc ttctccgagt
     1801 cgaagatcgc tcccgcgcgg ccgacgccgc cgaggtcgaa cgagtgaacc agctcgtcgc
     1861 gggtcatctt ctgccgatcg tcgcccggtg accagccgag caacgcgaga aagttgagaa
     1921 ccgcctgcgg cagatatcct cgctcgcgat attccagcag cgagaccgcc ccgtgccgct
     1981 tggaaagtcg cttgcggtcg ctgccgagga tcagcggcag atgggcaaaa tgcggcgtcg
     2041 gtcgctccag cgcctcgtac agcagtatct gcttcggcgt gttcgagatg tggtcgtcgc
     2101 cgcggatgac gtgggagatc cgcatctcga cgtcgtccga gacgaccgac atcatgtagg
     2161 tcggcgaacc gtccgagcgg agcaggatga agtcttcgat cgcgtcgccg gcgaatcgct
     2221 tgtcgccgtg gaccaggtcc cgccagccga tctcgcgatc gggtaccgcg aaccgcaccg
     2281 cgaacggttc gccggcatcg gtgcgttgtc gtgatcgcgc cgcgggcagt ccgcgacagg
     2341 tgcgggggta gacgtagtcc cggccgccgc ccggcgcggt tcgccgcgtc cgctccagca
     2401 gctccggcgg gcagaagcag cggtaggcat gaccggaggc gagcagccgc gccgcgtcgc
     2461 cggcgtgccg cgcgcgcgcg gcggactgga agaacggccc ctcatcgacg tcgagcccca
     2521 gccactcgat gccgtcgacg atcgcgcgct ccatcgcgtc ggaggaa
//
LOCUS       DASPGN010000151         3008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_259852, whole genome shotgun sequence.
ACCESSION   DASPGN010000151 DASPGN010000000
VERSION     DASPGN010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259852"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VD788_04195"
     CDS             complement(<1..187)
                     /locus_tag="VD788_04195"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HXV75496.1"
                     /translation="MSRLRFELVARDGAARLGRLDTPHGSVETPAFMPVGTQGAVKGV
                     SPDELRRAGSQIVLSNTY"
     gene            complement(184..1257)
                     /gene="queA"
                     /locus_tag="VD788_04200"
     CDS             complement(184..1257)
                     /gene="queA"
                     /locus_tag="VD788_04200"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF001140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HXV75497.1"
                     /translation="MRVDEFDYRLPPDRIAQRPAARRDGSRLLVLDRRTGELSHSRFD
                     RLADWLTAGDLLVLNDTRVFPARLHGRKPTGGRVELLLTERCDDGRPAPVEHWRCLIG
                     GAVRAGERLTIGPAFTATVIERGAELCRVRLESSSSATVGRRIDEHGVMPLPPYVKRP
                     DPASLAGEALSAEDRSRYQTLFARHRGAVAAPTAGLHFSERTFAALDAAGVERAFLTL
                     HVGAGTFAPVRVARVEDHVMHDEWFELGRETVDRLERTREHGGRVVAVGTTVVRALEH
                     AAGDGRPRPARGRCDLFIYPGYRFRVVDALLTNFHLPRSTLLMLVSAFAGREHVLAAY
                     DVAVGHGYRFYSYGDAMLIRSPR"
     gene            complement(1254..2156)
                     /locus_tag="VD788_04205"
     CDS             complement(1254..2156)
                     /locus_tag="VD788_04205"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HXV75498.1"
                     /translation="MGGTSRHRLGAHMSIGGGFERAVDRARSIDATALQIFVKSARQW
                     DARPIDPDAAARFRRRAAAAGLEQRTMAHASYLINPASAEPAVRRRSLAALADELERC
                     QALAVPYLVLHPGSHGGRGVEDGLLSLVDALDHVLSRPRRSRGKAAPGPPPTILLENT
                     AGQGATLGRRLEELAFVLDRCRFADRLGICFDTCHALAAGYEFRDARSYRATFDSIDR
                     SVGLHRLCGFHLNDSKHPLGSRRDRHEHVGRGQVGLEAFRLLLNDPRFADVPMVLETP
                     KGPDLAEDRVNLEVLRAMLPVARR"
     gene            complement(2220..>3008)
                     /locus_tag="VD788_04210"
     CDS             complement(2220..>3008)
                     /locus_tag="VD788_04210"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXV75499.1"
                     /translation="WRFVGAPTLTAYLDRLTRQLEPPSGRQPSGVRVEVFDQPRVWTL
                     ALPSGTVLLSRGALESVEDEAELVFVLAHELSHSAEPGAARRLARLGLREVAAGESDG
                     SPGVWARAAVDLIRVGHGRELEFAADAAGFAAAARLGYDLGAVRRYLDRLGTRQETGD
                     AAVAELVLAHPPVASRLRRLDAFVANRPPERRRVTRTNREVFRRVAGVQVLATRLAPV
                     EGLGERRTARQSVPERANERGVRPWLVLAALLVALAAIVGWYWW"
BASE COUNT          466 a         1105 c         1070 g          367 t
ORIGIN      
        1 gataggtgtt ggacaacacg atctggctgc ccgcgcggcg cagctcgtcg ggtgacacac
       61 ccttgacggc accctgggtc ccgaccggca tgaacgccgg tgtctcgacc gagccgtgcg
      121 gtgtgtcgag acggccgagc cgggcggccc cgtcgcgcgc gacgagctcg aatcggaggc
      181 ggctcacctg ggggacctta tcaacatcgc atcgccgtac gagtagaacc gatatccatg
      241 cccgacggca acgtcgtagg ccgcgagcac gtgctcccga ccggcaaacg cgctgaccag
      301 catcagcagg gtcgagcgcg gcaagtggaa attggtcaag agcgcatcga cgacgcggaa
      361 acgatacccc gggtagatga acagatcgca gcgtccacgg gcggggcgcg gccgtccgtc
      421 gccggccgcg tgctcgagcg cccggacgac ggtcgttccg accgcgacca cacgcccgcc
      481 gtgctcgcgg gtacgctcga ggcgatcgac cgtttcccgg ccgagctcga accactcgtc
      541 gtgcatcacg tggtcctcga ctcgcgcgac acgaaccggc gcgaacgtgc cggcgccgac
      601 gtgcagcgtg aggaacgccc gctcgacacc ggcggcgtcg agcgcggcga aggtccgctc
      661 gctgaaatgc aggccggccg tcggagcggc aaccgcgccg cggtgtcgcg cgaacagcgt
      721 ctggtacctc gaccggtctt ccgcggacaa cgcctcgccc gcgagcgagg cggggtcggg
      781 acgtttgacg tagggcggca acggcatcac cccgtgctcg tcgatccgcc gaccgacggt
      841 ggctgacgac gacgactcca atcgaacccg acacagctcc gcgccgcgct cgatcacggt
      901 cgcggtgaac gccgggccga tcgtcaaccg ctcgccggcg cgcaccgcgc cgccgatcag
      961 gcagcgccag tgctcgactg gagcgggacg gccgtcgtca caacgctcgg tgagtagcaa
     1021 ctcgacgcgc ccgccggtcg gcttgcggcc gtgcagccgc gccggaaaca cgcgggtgtc
     1081 gttgagcacc aacaggtcgc ccgccgtcag ccagtcggcc aggcggtcga atcgcgagtg
     1141 cgacagctcg ccggttcggc ggtcgagcac cagcagtcgg gagccgtcgc ggcgcgcggc
     1201 cgggcgttgc gcgatccgat cgggcggcag ccggtaatcg aactcgtcga ccctcaccgt
     1261 cgcgccaccg gcagcatcgc gcgcagcacc tccaggttga cgcggtcctc ggcgaggtcg
     1321 ggtcccttcg gtgtctcgag caccatcggc acgtcggcga agcgcgggtc gttgagcagc
     1381 aagcggaatg cctcgagacc gacctgtccg cggccgacgt gctcgtggcg gtcacgccgg
     1441 ctgccgagcg gatgcttgga gtcgttcagg tggaaaccgc acagccggtg caggccgacc
     1501 gatcggtcga tcgagtcgaa cgtcgcccgg tagctgcgcg cgtcgcggaa ctcgtagccc
     1561 gccgcgagcg cgtgacacgt gtcgaagcaa atgccgagcc ggtcggcgaa ccgacaccgg
     1621 tcgagtacga acgccagctc ctcgaggcgg cgcccgagtg tcgccccctg gccggcggtg
     1681 ttctcgagca ggatcgtcgg cggcgggccc ggagccgcct tgccgcgcga gcgccgcggg
     1741 cgcgacagca cgtggtccaa cgcgtcgacc aggctgagca gaccgtcctc gactccccgg
     1801 ccgccgtggg agccgggatg caggacgagg tacgggacgg cgagcgcctg gcaccgctcg
     1861 agctcgtcgg cgagcgcggc gagcgagcga cgccgtaccg cgggctcggc actcgccggg
     1921 ttgatcaggt agctcgcgtg agccatcgtc cgttgctcga gaccggcggc ggccgcgcgg
     1981 cggcggaagc gcgccgccgc gtcgggatcg atcggcctgg catcccactg gcgggcggac
     2041 ttgacgaaga tctgcagtgc cgtcgcgtcg atgctccgcg cgcgatcgac cgcccgctcg
     2101 aacccgccgc cgatcgacat gtgcgcaccg agacggtgcc gtgacgtgcc gcccatgacg
     2161 cgcgatcgta gcgcgtcgcg tggcgcgagg ctatgccgag gcccgcccgg cgaccggccc
     2221 taccaccaat accaaccgac gatcgccgcc agcgcgacga gcagcgccgc cagcaccagc
     2281 caaggccgaa cgccgcgctc gttcgcccgc tccggaaccg actggcgggc ggtccgccgc
     2341 tcgccgagtc cttcgaccgg cgcgagcctc gtcgcgagca cctgcacgcc ggcgacgcga
     2401 cggaagactt cgcgattggt gcgggtgacc cgccggcgct cgggcggacg gttggcgacg
     2461 aatgcatcga ggcggcggag acgggacgcg accggcggat gggccagtac cagctcggcc
     2521 acggcggcat cacccgtctc ctgtcgcgtg ccgagccgat cgaggtagcg ccgcaccgcg
     2581 ccgaggtcgt agcccagccg ggcggcggcg gcgaagcccg cggcatcggc cgcgaactcg
     2641 agctcgcggc cgtgacctac gcgtatcagg tcgaccgcgg cgcgtgccca cacgcccggc
     2701 gagccgtccg actcgcccgc cgcgacctcg cgcagaccga gccgcgccag ccgccgggcc
     2761 gctccgggtt cggccgagtg cgacagctcg tgcgccagca cgaataccag ctcggcctcg
     2821 tcctcgacgc tctcgagcgc gccacgactg agcagcaccg tcccggacgg cagcgccagc
     2881 gtccacaccc gcggctggtc gaacacctcg acccgcacac cgctcggctg tcgccccgac
     2941 ggcggttcga gctgtcgcgt cagccgatcg aggtatgccg tcaacgtcgg cgcaccgacg
     3001 aagcgcca
//
LOCUS       DASPGN010000152         8901 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_259956, whole genome shotgun sequence.
ACCESSION   DASPGN010000152 DASPGN010000000
VERSION     DASPGN010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8901
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259956"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="VD788_04215"
     CDS             <1..1589
                     /locus_tag="VD788_04215"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXV75500.1"
                     /translation="LARILEGRGFNVALQKLDPYINVDPGTMSPFQHGEVFVTDDGTE
                     TDLDLGHYERFASMTATKNHNFTTGKIYGSVIEKERRGDYLGATVQVIPHITDEIKQS
                     IVKISKGIDIQIVEIGGTVGDIESLPFLEAIRQFWLDVGRSNAIFVHLTLVPFIATSG
                     ELKTKPTQHSVRELRAIGIQPDILLCRSDRPLPADIKRKIALFCNIGEDAVISATDVS
                     NIYEVPLRLSDEGIDEIVVKLLDLPYRKKDLSRWEDLVERIRHPHDEVTIGIVGKYVS
                     YEDSYKSLSEALLHGGVGSRLKVHLKWIEAEDAENGKLRTELADVDGILIPGGFGIRG
                     VAGMIQAVRFAREDKIPFFGICLGLQCAVIEAARNLCGIASADSTEFDESATDRVIYK
                     LRDLLGVDAMGGTMRLGAYPTDLADGSFAHQAYGKRSISERHRHRYEVNQEFLPRLEA
                     AGVRFTGMSPDRKFVEIVERDDHPWFLACQFHPEYKSRPLDPHPLFREFVAAAYRHRQ
                     LRLEGLSLGTSRAYVADRNS"
     gene            1618..2388
                     /gene="kdsA"
                     /locus_tag="VD788_04220"
     CDS             1618..2388
                     /gene="kdsA"
                     /locus_tag="VD788_04220"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HXV75501.1"
                     /translation="MLVIAGPDVIESEAHAVGVAVRLARIAEERGLPLVYKSSFDKAN
                     RTSVASARGPGLERGLRVLARVKRESGLPLTTDFHLPEQAGPVAEVVDLLQVPAFLCR
                     QTDMLLAAGRTGRAVNVKKGQFLAPADAAHIVAKVRSTGNDKVMLTERGSSFGYNNLV
                     VDFRSLPRLRELGVPVCFDATHSVQLPGGLDGRSGGEREFIAHLARAAVAVGVDALFF
                     EVHDDPDRALCDGPNQYRLDDFPALLDVLTSIDRCAKG"
     gene            2373..2825
                     /locus_tag="VD788_04225"
     CDS             2373..2825
                     /locus_tag="VD788_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75502.1"
                     /translation="MRKGLIAAGGGLVLLVAGSCGETSPEEPLRTALLLFEIAAQKPR
                     DEAMLARVFEMQDDEADRAKLGDALDALPAAAGRPEVRLVDRFDELGRVAVDLTVLLP
                     GGSEADYTVQLQRMGESDWRVIEFHGPGVSWPPRPVPGGEGLSTRPDP"
     gene            2863..3834
                     /locus_tag="VD788_04230"
     CDS             2863..3834
                     /locus_tag="VD788_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HXV75503.1"
                     /translation="MTTKPQPRPTARRVFAIESRAIDDLASRLDASFDRAVELLLGCR
                     GRVIVTGMGKSGIIAQKIAATLSSTGCPAYFLHPAEAVHGDLGMIVSGDLLLALSNSG
                     ETAELVRLLELVRRLGARVVALSGDPRSTLARFADVHLDVGVDREACGLDLVPTASTT
                     AALAMGDALAVATYEQRGFSATDFARFHPGGRLGRKLQQVQQLMHRGQDVPRVAAAAT
                     LAATVEEIDRKKLGMTCVVDDDGRLCGVLTDGDLRRRMLRVERPMEGTAGEAMTRGPS
                     TIGAEALASEALKMMEDRKITSLPVVDPSGLVLGVIQIHDLWRTDLF"
     gene            3881..4435
                     /locus_tag="VD788_04235"
     CDS             3881..4435
                     /locus_tag="VD788_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HXV75504.1"
                     /translation="MTIESNAPTSEVVDRARKIRLVLFDSDGTLTDGRIIMTSGGEEA
                     RAFDVRDGHGVRMGQQAGLVFGVISGRESTVLQRRAEELEFEEIHQRVLDKEQRLKEI
                     LERRGLDASAVCFMGDDLIDLPVMRRCGLAVAPADAVAEVLDCAHHVTRRPGGRGAAR
                     ELIDLLLRVSGSWERVTARYFGEP"
     gene            4459..4908
                     /locus_tag="VD788_04240"
     CDS             4459..4908
                     /locus_tag="VD788_04240"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HXV75505.1"
                     /translation="MRAMWMVICIVFAFAFLGFLVSNVDTRVPVTLGMTDYEGVPLSV
                     VVVVAIVFTAVLVGIGAVAEGTKDRLENRRLRRELHKLETEINYLRTQPTSAPAAPPS
                     ATSTSAGATDADEADHGQPPSAPVYGDAEGEWAGDADDDVYSGGRAV"
     gene            complement(4916..7306)
                     /locus_tag="VD788_04245"
     CDS             complement(4916..7306)
                     /locus_tag="VD788_04245"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXV75506.1"
                     /translation="MRHDRPTEIPSTLLAGRLEGISVPDLLWSLCRHRSTGVLRVQHA
                     GIEKRVYVEGGQIVFASSSDPDDRLGERFLRAGWITIEQLDRAVERLAGGKRLGTLLV
                     EDGSLEAGQLVTGVLNQVESIVLDVFEWEDGDYRFDEGLPTEEMIKLEIKTSELLLRG
                     IRQLRSFSRVRRSVGSTQKIYGLTEEWESLLDGVSLTAGEQSLVGQLGEGELSIEALC
                     REVFLSNFEIYQTMWALKVLGVVEEREELWMAPSDVTVHGSLDQIDFTELLVSLARSN
                     ETGVLYVTRGERERSFHIAGGRCVFATSSNPDDGLMAYLLRRGVISLRDREETAKRLL
                     SNKRVGAILRDLGVIDQQDLQSMVREQLSEVVYDTFRWTDALYAFVPGPLPTVEEITL
                     EADLDALVASGIRRVESWSRVWHGCGGIDVTLELKPSYLAVLDAMAAGPEEWEVIAAL
                     KKPCSPREVCARTGIGDFRVCQILWTLRVLGGVEAVGEVAVDLPLEPSAELDAAAGAA
                     VKRRSRALLSEDRPGRPLIADQAIEQAIVGMERDLDDQPVEVAGEPPGIDRPELRPSA
                     VVRAEREADESERRSPEATQALTRDEVESAIAPAVDAAVDEPGADTETEPPPDATQVI
                     PREVVEAAVSGADEPAVPARPNEHEHDSATTEPDLVTAAAAVDDDGGTARDWELPPEV
                     DSAIARFNAAQRVVYRTIRAEVGAGAANFIRSCCSQLDSIDADVVAGVDLNADGSWET
                     DGLREAVRKMRSEDPWLIYQRLIEGEIDRLRPFIGDAKAAELQKQVELVGQPETGR"
     gene            complement(7414..7617)
                     /locus_tag="VD788_04250"
     CDS             complement(7414..7617)
                     /locus_tag="VD788_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HXV75507.1"
                     /translation="MPIDRRLLEILRCPACHGEIRPVDDDSGLECRSCGRIYPIRDGI
                     PIMLVEEASPPTRDDSETTGSRT"
     gene            7678..8820
                     /locus_tag="VD788_04255"
     CDS             7678..8820
                     /locus_tag="VD788_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXV75508.1"
                     /translation="MIALPPDLRTSLAASGRDLKIVIIRLGAMGDVLRTLPPVRLLRS
                     ALPLASIVWIVERPWDCLLDGHVDIDEVRIAPRREWQRLSRSPREWPRLLAALRAFRG
                     ELAAIRADLAIDFHGNLRSGWVAAWSKAPVRLGYAGHQQKEGNRWFTTHRVGAADRRT
                     PRMERNLALIRALGLPDGPLPGGALPLVAAGRAAADAVAAALPARVGGLAVLSPGSSV
                     RQAYKRPPVELLATACRRLVELDLTPLVVWGPGEQADAAAVIQRSAGTAVLAPPTGLA
                     ALAALLDRARIFIGGDTGPLHMACAVGCPVVGIYGPTDPRVNRPWGVTHRVVAPADRR
                     YTGIKRIDRAAGGFAGITAAQIESAVDELLAGERGRNRPLVPSQPV"
BASE COUNT         1350 a         3079 c         3042 g         1430 t
ORIGIN      
        1 cgctcgcgcg cattctcgag ggacgcgggt tcaacgtcgc gctgcagaag ctcgacccgt
       61 acatcaacgt cgatcccggc acgatgagcc cgtttcagca cggcgaggtg ttcgtcaccg
      121 acgacggaac cgagaccgac ctggacctcg gtcactacga gcggttcgcc tcgatgaccg
      181 cgaccaagaa ccacaacttc accacgggca agatctacgg ctcggtgatc gagaaggagc
      241 ggcgcggcga ttacctcggc gcgaccgtgc aggtgattcc gcacatcacc gacgagatca
      301 agcagtcgat cgtcaagatc tccaagggga tcgacattca gatcgtcgag atcggcggga
      361 ccgtcggcga catcgaatcg ttgccgttcc tcgaggcgat tcggcagttc tggctcgacg
      421 tcggccggtc caacgcgatc ttcgtgcact tgacgctggt tccgttcatc gccacctcgg
      481 gcgagctcaa gaccaagccg acccagcact cggtgcgcga gctgcgcgcg atcgggatcc
      541 agcccgacat cctgctctgt cgcagcgacc ggccgctgcc ggccgacatc aagcgcaaga
      601 tcgcgctgtt ctgcaacatc ggtgaggatg cggtgatcag cgcgaccgac gtgagcaaca
      661 tctacgaggt gccgttgcgg ctgtccgacg aaggaatcga cgagatcgtc gtcaagttgt
      721 tggacctgcc ctaccgcaag aaggacctgt cccgctggga agacctggtc gagcggatcc
      781 ggcatccgca tgacgaggtg acgatcggga tcgtcggcaa gtacgtctcc tacgaggact
      841 cgtacaagtc gttgagcgag gcgctgctgc acggcggggt cggatcgaga ctgaaggtcc
      901 atctcaagtg gatcgaggcc gaggacgcgg aaaacggcaa gctccggacc gagctggccg
      961 acgtcgacgg catcctgata ccgggcggtt tcggcatccg cggggtcgcc ggcatgatcc
     1021 aagcggtgcg cttcgcgcgc gaggacaaga tcccgttctt cggcatctgc ctcgggctgc
     1081 agtgcgcggt gatcgaggcc gcccgcaacc tgtgcggcat cgccagcgcc gactcgaccg
     1141 agttcgacga atcggcgacc gaccgggtga tctacaagct ccgggatctg ctcggggtcg
     1201 acgcgatggg tggcacgatg cgcctgggcg cctatcccac cgacctggcc gacgggtcgt
     1261 tcgcgcacca ggcctacggg aagcgctcga tctcggagcg gcaccgtcac cgttacgagg
     1321 tcaaccagga gttcctgccg cggctcgagg ccgccggggt gcgcttcacc ggaatgtcgc
     1381 ccgatcgcaa gttcgtcgag atcgtcgagc gagacgatca tccttggttc ctcgcctgtc
     1441 agttccatcc cgagtacaag tcgcgaccgc tcgatccgca cccgttgttc cgcgagttcg
     1501 tcgccgccgc ctaccgccac cggcagctgc gcttggaagg gctgtcgctc ggaacatcgc
     1561 gtgcctacgt cgccgatcga aatagctgac gccgtacgcg tcgggggcgg cgcgccgttg
     1621 ctggtcatcg ccggcccgga tgtgatcgag agcgaggcgc atgcggtcgg cgtggccgtc
     1681 cggctggccc ggatcgccga ggagcgcggg ctgccgctgg tctacaagtc gtcgttcgac
     1741 aaggccaacc gcacgtcggt cgcctccgcc cgcggtcccg ggctcgagcg gggcttgcgc
     1801 gtgctcgctc gcgtcaaacg ggagagcggc ctgccgctga cgaccgattt ccatctgccg
     1861 gagcaggccg ggccggtggc cgaggtcgtc gacctgttgc aggtcccggc gtttctttgc
     1921 cggcagaccg acatgctgct ggcggcgggc cgaaccggcc gtgcggtcaa tgtcaagaag
     1981 ggccagttcc tcgcgccggc cgatgccgcg cacatcgtcg ccaaggtgcg gtcgaccggc
     2041 aacgacaagg tgatgctgac cgagcgcggc agctcgttcg gatacaacaa cctagtcgtc
     2101 gattttcgct cgctgccgcg cctgcgcgag ctcggcgtgc cggtctgctt cgatgctacg
     2161 cactccgtgc agctgccggg cgggctcgac ggacgctcgg gcggcgagcg cgagttcatc
     2221 gcgcacttgg cgcgcgccgc cgtcgcagtc ggcgtcgacg cgctgttctt cgaggttcac
     2281 gacgatcccg atcgtgcgct gtgcgacggg cccaatcagt acaggctcga cgacttcccc
     2341 gcgttgctcg acgtgctgac ctcgatcgac cgatgcgcaa agggctgatc gccgccggcg
     2401 gcgggctggt gttgctcgtc gcgggctcct gcggggagac ctcgccggag gagccgttgc
     2461 ggaccgcgtt gttactgttc gagatcgccg cgcaaaagcc gcgcgacgag gcgatgctcg
     2521 cacgggtctt cgagatgcag gacgacgagg ccgatcgcgc caagctgggc gacgcgctcg
     2581 acgcgctgcc cgcggcagcg gggcggcccg aagtgcggct cgtcgatcgg ttcgacgagc
     2641 tgggacgggt cgccgtcgac ctgaccgttc tcctgcccgg cgggagtgaa gcggactata
     2701 cggtccagct ccagcgaatg ggtgagtcgg actggagagt gatcgagttc cacgggcccg
     2761 gcgtctcttg gccgccgcgt cccgtcccgg gcggggaggg cctgtcgacg cgaccggacc
     2821 cgtgacccgc ggcacgcgcc atcgtctatc atgggggcgc cgatgacgac gaaaccccag
     2881 ccgcgaccga ccgcccgtcg ggttttcgcg atcgaatccc gcgcgatcga cgacttggcg
     2941 tcgcgcctcg acgccagctt cgaccgcgcg gtcgagttgt tgctcggttg ccgcggccgc
     3001 gtgatcgtca ccgggatggg gaaatccggc atcatcgcgc agaagatcgc ggcgacgctg
     3061 tcgtcgacgg gatgccccgc gtatttcttg catcccgccg aggcggttca cggcgacctc
     3121 ggcatgatcg tcagcggcga cctgctgctg gccctgtcga actcgggcga gacggccgag
     3181 ctcgtccgct tgctcgagct ggtccgacgt ctcggcgcgc gggtggtcgc gctgagcggg
     3241 gatccgcggt cgacgttggc ccgcttcgcc gacgtccatc tcgacgtcgg ggtcgaccgc
     3301 gaggcctgcg gtttggacct ggtgccgacc gcgtcgacga ccgccgcgct ggcgatgggc
     3361 gacgcgctgg ccgtcgcgac ctacgagcag cgcggctttt ccgcgaccga cttcgcgcgg
     3421 ttccatcccg gtggccggtt ggggcgcaag ctgcagcagg ttcagcagct gatgcaccgc
     3481 gggcaagacg tcccgcgcgt cgccgcggcg gcgacgctcg ccgccaccgt cgaggagatc
     3541 gatcgcaaga aactcggcat gacctgcgtg gtggatgacg acggccgcct gtgcggcgtg
     3601 ctgaccgacg gcgacctgcg tcgccggatg ttgcgcgtcg agcggccgat ggagggcacg
     3661 gccggcgagg cgatgacccg cggcccgtcg acgatcggcg ccgaggcgct ggccagcgaa
     3721 gcactgaaga tgatggaaga ccgaaagatc acctcgctgc cggtggtcga cccttccgga
     3781 ctggtgctgg gcgtgatcca gatccacgat ctgtggcgga ccgatctgtt ttgaccgacc
     3841 gtgcgatcgc tggggtatgc tgtcgacgga gcccggacag gtgaccatcg aatcgaacgc
     3901 tcccacctcc gaggtggtcg accgcgcgcg caagatccga ctcgtgctgt tcgactccga
     3961 cggcacgctg accgacggcc ggatcatcat gacgagcggt ggcgaggagg cgcgcgcctt
     4021 cgacgtgcgc gacggccacg gcgtgcgcat gggtcagcag gcgggactgg tcttcggcgt
     4081 catctccggc cgcgaatcga ccgtgctcca acgccgcgcg gaagagctcg agttcgaaga
     4141 gattcaccag cgcgtgctcg acaaggagca gcgactgaag gagattctcg agcgacgtgg
     4201 gctcgacgcc tcggcggtct gcttcatggg cgacgatctg atcgacctgc cggtgatgcg
     4261 ccgctgcggt ctggcggtgg ctccggccga cgcggtcgcc gaggtgctgg actgcgcaca
     4321 ccacgtcacg cgccggcccg gcggccgggg tgcggcgcgg gagctgatcg atctactgtt
     4381 gcgcgtgagc ggcagctggg aacgggtgac cgcccgttac ttcggcgagc cgtgacggcg
     4441 cggggagacg gacgagccat gcgcgcaatg tggatggtga tctgcatcgt ttttgccttc
     4501 gctttcctcg gcttcctggt cagcaacgtc gacacccgtg tgccggtgac gctcgggatg
     4561 accgactacg agggcgtgcc gctgtcggtc gtggtggtgg tggcgatcgt gttcaccgcg
     4621 gtcctggtcg gaatcggcgc ggtcgccgaa ggaacgaagg accggctgga gaaccgccgg
     4681 ttgcgccgcg agctgcacaa gctcgaaacg gagatcaact atctgcgcac ccagccgacg
     4741 tcggcgccgg cggctccgcc gtccgccacg tcgaccagcg ccggcgcgac cgacgcggac
     4801 gaggcggatc acggccaacc cccgtcggcg ccggtctacg gcgacgcgga gggcgaatgg
     4861 gcgggcgacg cggacgacga cgtctactcg ggtgggcgcg ccgtttgagc cggggtcagc
     4921 ggcccgtctc gggctgtccg accagctcca cctgcttctg cagctcggcc gccttcgcgt
     4981 cgccgatgaa cggacgcagc cggtcgatct cgccctcgat caggcgctga tagatcagcc
     5041 acggatcctc ggagcgcatc ttgcgcaccg cctcgcgcag cccatcggtc tcccatgacc
     5101 cgtcggcgtt gaggtcgacc ccggccacca cgtcggcatc gatcgagtcg agctgggagc
     5161 aacacgaacg gatgaaattg gcggcgccgg cgcctacctc cgcacgaatc gtgcggtaga
     5221 cgacgcgctg cgcggcgttg aagcgcgcaa tcgcgctgtc gacctcgggc ggcagctccc
     5281 agtcgcgggc ggtgccgccg tcgtcgtcca ccgccgccgc ggcggtcacc aggtccggct
     5341 cggtcgtcgc cgaatcgtgc tcgtgctcgt tcggccgcgc gggaaccgcc ggctcgtcgg
     5401 caccgctcac ggccgcctcg acgacctcgc gcgggatgac ctgcgtcgcg tcgggcggcg
     5461 gctcggtctc cgtgtcggcg ccaggctcgt cgacggccgc gtcgaccgcc ggcgcgatcg
     5521 cgctctccac ctcgtcgcgg gtgagcgctt gcgtcgcctc gggcgaccgc cgctccgatt
     5581 cgtccgcctc gcgctcggca cgcaccaccg ccgacggtcg cagctccggc cgatcgatcc
     5641 ccggcggctc ccccgcgact tcgaccggct ggtcgtcgag gtcacgttcc atgccgacga
     5701 tcgcctgctc gatcgcctgg tcggcgatca acggccgtcc gggcctgtcc tcgctcaaca
     5761 gcgcgcgcga ccgacgcttg acggcggcgc ccgccgcggc gtcgagctcc gcgctcggct
     5821 cgagcggcag gtcgactgcg acctcgccga ccgcttcgac tccgccgagt acgcgcaggg
     5881 tccagaggat ctggcacacg cgaaagtcgc cgattccggt tcgggcgcag acctcgcgcg
     5941 gagagcacgg ctttttcaag gccgcgatca cctcccattc ctccggcccc gcggccatcg
     6001 cgtcgagcac cgcgaggtag ctcggcttca actcgagtgt cacgtcgatg ccgccgcaac
     6061 cgtgccagac gcggctccac gactcgaccc ggcggatccc gctcgcgacc aacgcgtcga
     6121 gatccgcctc gagcgtgatc tcctcgacgg tcggcaacgg tccgggcacg aaggcgtaga
     6181 gcgcgtcggt ccagcggaac gtgtcgtaga ccacctcgct gagctgctcg cggaccatcg
     6241 actgcaggtc ctgctggtcg atcacgccga gatcgcgcag gatcgcgccg acacgtttgt
     6301 tcgacagcag ccgcttggcc gtctcttcgc ggtcgcgtaa cgaaatgacg ccgcgccgca
     6361 gcaggtaggc catcagcccg tcatccgggt tgctgctggt ggcgaacacg caacgaccgc
     6421 cagcgatgtg aaacgaccgt tctcgttcgc cacgggtgac gtacagcacg ccggtctcgt
     6481 tcgagcgcgc caagctgacc aacagctcgg taaagtcgat ttggtcgagc gacccgtgga
     6541 cggtgacgtc gctcggcgcc atccacagct cttcacgctc ctcgacgacg cccagcacct
     6601 tcaacgccca catcgtctgg tagatttcga agttcgacag gaagacctcg cggcacaacg
     6661 cctcgatgct gagctctcct tcgcccagct gtccgaccaa cgactgctcg ccggcggtca
     6721 gcgacacgcc gtccaacagc gactcccatt cttcggtcag gccgtagatc ttctgtgtcg
     6781 agccgacgct gcgacgaacc cgactgaacg agcgcagctg ccggatgccg cgcagcagca
     6841 gctcgctggt cttgatttcg agcttgatca tctcctcggt gggcaggcct tcgtcgaagc
     6901 ggtagtcgcc gtcttcccac tcgaagacgt cgagcacgat cgattcgacc tgattcagca
     6961 cgccggtcac gagctgaccg gcttcgagac tgccgtcctc gaccaagagc gtgcccaggc
     7021 gctttccgcc ggccagccgc tcgaccgcgc gatcgagctg ctcgatcgtg atccagcccg
     7081 cccgcaggaa ccgctcgccg agccggtcgt ccgggtcgct ggacgaggcg aagacgatct
     7141 gaccgccctc gacgtagacc cgcttttcga tgcccgcgtg ctggacccgc agcacgccgg
     7201 tgctgcggtg tcggcacaac gaccagagaa gatcgggcac gctgatgcct tccagccggc
     7261 cggcgagcaa cgtcgacggg atctcggtcg gtcgatcgtg tcgcatgggt cgtgaccctt
     7321 cccctaccgg acgagggacc ggcctcgacg gccggtcgga cacccgccgg gatccgaaaa
     7381 ctcagagcaa aggtagtccc gaagataaga ttttcaagtc cgggaaccgg tcgtctccga
     7441 gtcgtcgcgg gttggcggcg atgcctcctc gaccagcatg atcgggatcc cgtcgcggat
     7501 cgggtagatc ctgccgcagc tccggcactc gagccccgag tcgtcgtcga cgggccgtat
     7561 ctcgccgtga caggccgggc agcgcagaat ctcgagcaaa cgtcgatcga tcggcaccga
     7621 atccctcccg ggtggggaac ggcggaagca taccagaacg acatctatac tggcccggtg
     7681 atcgccctcc cgcccgacct gcgcacgtcg ctcgccgcgt ccggccgcga tctgaagatc
     7741 gtgatcatcc gtctcggggc gatgggcgac gtcctccgta ccctgccgcc ggtgcggctg
     7801 ctgcgatcgg ccctcccgct ggcgtcgatc gtctggatcg tcgaacgccc gtgggactgc
     7861 ctgctcgacg ggcacgtcga catcgacgag gtgcggatcg ctccgcgccg ggaatggcaa
     7921 cgtctctccc gttcaccgcg tgagtggccg cggttgctcg ccgcgctgcg tgccttccgc
     7981 ggcgagctgg ccgcgatccg cgccgatctc gccatcgact tccacggcaa cctgcgcagc
     8041 ggttgggtcg ccgcgtggtc caaagcgccc gtccgtctgg gctacgcggg tcaccaacaa
     8101 aaggaaggca accgctggtt caccacccac cgtgtggggg cggccgacag acgcacgccg
     8161 cggatggaac gcaacctggc gctgatccgt gccctcggcc tgcccgacgg gccgttgccg
     8221 ggcggtgcgc tgccgttggt cgccgcaggt cgagcggcgg ccgacgccgt cgccgccgcg
     8281 ttgccggcgc gagtcggcgg actggcggtg ctgtcgccgg gttcgagcgt ccgccaagcc
     8341 tacaaacggc cgccggtcga gctgctcgcc accgcctgcc ggcgtctcgt cgagctcgac
     8401 ctcacgccgc tcgtcgtctg gggcccgggc gaacaggcgg acgcggctgc cgtcatccag
     8461 cggtcggcgg ggacggccgt tctagcgccg ccgaccgggc tcgccgcgct ggcggcactg
     8521 ctcgaccgcg cccgcatctt catcggcggc gacaccggcc cgctgcacat ggcttgtgcg
     8581 gtcggctgtc cggtcgtcgg catctacggc ccgaccgacc cgcgagtcaa ccgaccgtgg
     8641 ggagtgaccc atcgcgtcgt cgccccggcc gatcggcgtt acacgggtat caagcggatc
     8701 gatcgggctg ccggcgggtt cgccgggatc accgcggcgc agatcgaaag cgcggtcgac
     8761 gagctgctcg cgggagaacg cggccggaac cggccgctag tgccatccca gccggtgtag
     8821 cccgatcagc aggcggttac cccggcgagc tcgctcgaga cgccgttggt cgtccgcatc
     8881 gccgtcggca taggcctcgt a
//
LOCUS       DASPGN010000153         9814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_260292, whole genome shotgun sequence.
ACCESSION   DASPGN010000153 DASPGN010000000
VERSION     DASPGN010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9814
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260292"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VD788_04260"
     CDS             complement(<1..379)
                     /locus_tag="VD788_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75509.1"
                     /translation="MSREPFNIGSRFRGARDREARRLGTKKVVEGVPLAKLIRRRLVD
                     RPEIVMVAAPRSIHAEKFRRLKTTLTNLPDGRPQVMLVSSAVPKDGKSTISINLGLAF
                     AADPEVKTLLIDADLRRGSVSRFV"
     gene            complement(376..1914)
                     /locus_tag="VD788_04265"
     CDS             complement(376..1914)
                     /locus_tag="VD788_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75510.1"
                     /translation="MEAKANQQRLVLFELIDNLARNWWTVVAGFSVGLSVAVVALHFT
                     PKVYEAAAQLDITSEQLPDGVIADTVPGDPATKLTEIRQNVMSERNLDRIITEEFGES
                     PSEKERDALRGRIRSGVRVTLPRWEDYVQIAFQDADPYRAARIANLLATFYVDENLKF
                     RQDRASVTAETFEKLAAERWTDIQRLKRELETFDAGNPYQNEAQRPINQQALEDARLA
                     LDANLKAQAELQLQIDFLEADQAQAGMLDSFVPVPGAVAGSGDAGLSVLEIELQQLLI
                     DYSENHPAVRKKKAEIERYEKQQESQRSTAPSEPVADGEKARSETSRAWDVQIASARA
                     ELNRIKAETETLRSDIAKYQRRLSETPGVERRREELVDEIDSLTAEYRRLRENAIKAG
                     TSELVEQQGQGDQFQIIAGAAPPSNPIHPRPLLFYGVGIAVGLLLFSGPVLGRTILAP
                     VVSSEARILETAAIPVLVSIPSIPTPTIARRRRLLLLRNVALSLLSSAVLGTVLGLKL
                     KDVL"
     gene            2368..3552
                     /locus_tag="VD788_04270"
     CDS             2368..3552
                     /locus_tag="VD788_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75511.1"
                     /translation="MVCAFASGSTSEARVTFDPSLFLTVETTDNVAFANSTQPKTSDS
                     NATLGVTLPVRRETARGWLEFLYSTGFSRYREITAFDDINHLLEFRSYREPNPATRLN
                     AEACYLRTQQQNRGYRVRSCTELFTTERTRRELFGGTVDYNRRLGRLWSWNVELQAAV
                     ANFDAIEDFDPGDTGLVVEDKVGYRASAGIARRFGRRLRLGTSYSYGVVFQDLSPDVE
                     THRLRLDVEHEATRRLQYRLQVGAYYRSSDEVDEQMQDDLRTSGVEFLFELQYGESKG
                     YRIGPVVLELGAGIQPTAGSGLQGTSTDAVVSAGLSPVRSQRWNWQFESTYGVRIPTE
                     SDAVDIRTLRGSAWIERELGRAFGARLRGYFSDQMADDPTYAVSLYIVEISLAWYFLR
                     HR"
     gene            3779..5941
                     /locus_tag="VD788_04275"
     CDS             3779..5941
                     /locus_tag="VD788_04275"
                     /inference="COORDINATES: protein motif:HMM:NF025182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HXV75512.1"
                     /translation="MLITRARLVPLCVVALAAGAGAARAQSSSYPPSTVITDISFDMT
                     TLQQHAPGSDNWIVTWSDDGHQYTSFGDGGGFGGTDNDGRVSMGVARIEGGKDSYVGI
                     NVNGGKNPESGNSTWSSTGGKSLGIISIDLSGSGGQRGTLFMYRNGTGSEAGAFEQTE
                     LYRSLDHGRSWSYTGVRWTDPGFFSPSFLQFGQDYAGARDEYVYSYAPTNQNTTWEVQ
                     PAGEILLFRVRKDQLASPGAWEYFSGTASNPSWSSNLSNATPVFADPANGVMRTSVSY
                     NAPLGRYILITQQVSRFEADDYHIGIYDAPQPWGPWTTVFFGNPRVDLSPSLNSGSKT
                     VFWNLSNKWLSADGRDFVMVYTGPGPDNMGTVEGRFVTVTDTSPPSAPQNLVATGSSD
                     DSIGLSWSASSDPQSGISHYRIRRDGTTVAQPPGTTFVDTGLAEATAYTYQVSAVNGA
                     GLESTAATTVASTLADLTPPALTSVVAGGPNQVTVTFSEPVEVLTATNAANYQIDNGI
                     AVAGAALDAAGLVVTLQTSSHGVGPTYTLTVNGVRDRAAAPNTIASNTQASYTFADQL
                     TISSISVASGEPYVVAQPLSNGSLAYIDRTFGYSNVPQFLLGAQYIRTANDDKQSQGS
                     AFLSFQVNRAVKVYVAHDDRYGTKPSWLQSFLDTGIGISLDVSFTLFEQPFPAGTVTL
                     GGNIEPPETQGYSMYTVIVVADPGGDTAPAPPTGVDLN"
     gene            6030..7058
                     /locus_tag="VD788_04280"
     CDS             6030..7058
                     /locus_tag="VD788_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXV75513.1"
                     /translation="MSPSLSAQAGRTAERGRGRTRSRVKLAVKALVTLGLLALLLDRV
                     NLDDSVRALRRAQPVWIVLGTLVCSTGFLASSQKWHGLLAAMGVAIDRWAALRIYAIG
                     HFASSFFPGTIGGDLLRCNMVRPVTGGYVRAFASVLAERASGVVTMVALAIAAVAWRP
                     DRLATPPVLTLVGIGSAGVVLALLLALNRRLAVAVTYRLRRGSLRRVVRRLYELHRIL
                     RSLSASALAVALGWSVGFYFGSGLLMYTCCRAFERPIGVLDAVSVVVVVSILMLIPIS
                     LGGLGLRQVGDVMMLDLFGVDPGSALVISLLRQAINYFYTLIGGVFFVLWTGAELGPH
                     VSPREEGL"
     gene            7058..8596
                     /locus_tag="VD788_04285"
     CDS             7058..8596
                     /locus_tag="VD788_04285"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXV75514.1"
                     /translation="MFDRASSAFSALKGPEPFDACVIGSGFAGIVVAKGLVERGARTL
                     LLESGQGMLNWLLDKRLHELAAYEFSGDTDYPLKRTKARIVGGNSNFWTGRAERFHPS
                     DFADHPYLPSPWPIGYDELEPYYVRAERTLRVRAADFSEFAAPRSENPAVPPRPNISD
                     LRAILARAGVTLDYSATATPRRAFRFFRMNHEVLPEVRRNPRLTLVSNATVTRLLVEP
                     DGLVGGAEVRALDRTTKVARAKVFVVCCGGFESPRLLLLSRSESFPNGIGNRFDRVGR
                     GFNEHASVNFYGAMTHRRGTVLPRHKIGRSHQFYERYRNEGLGALLLNAIQSWVFPNH
                     LLKFRLVDMPRYAAFLAKRVIRPELYLGTTIEMTVEDSNRITLSDRMTDHFGNPLAHM
                     IFNYSTQDRLLMERSRELVREIYSKLGISEIREGPDNFSMHHQGTCRMGDDPKVSVVD
                     RDLRVHESPNLYVCGAENLVSGSAVPPTLTLVALAHRLADHLGEALHRLPSRSYAARE
                     SGRP"
     gene            complement(8619..>9814)
                     /locus_tag="VD788_04290"
     CDS             complement(8619..>9814)
                     /locus_tag="VD788_04290"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HXV75515.1"
                     /translation="IPSFDSLGNYYGYVTHLCGYKMGKHEGKITGLAAHGKPRYKPVL
                     ERFIRYEHGTMRNTGNAFRGAAVQKLKRALPGDFDKADLSASIQDLSEEICSQYVAYW
                     AKKTGKTKIALAGGVVANVKINQRIHEIPGAESVFVFPAMSDEGLAAGAALLLCSDKR
                     PGLAMAGKKCIDHVYLGPSFSDREIGDALSAAGVDYTHHDDVEGEIARLINRGYVVAR
                     FHGAMEYGPRALGNRSILYRPDDPSVNDWLNERLKRTEFMPFAPSTITEDAHLSFKNL
                     PGATDTARYMTITFDCTKEMAERCSGVVHVDNTARPQLVARSDNESYYRILTEFKWLT
                     GLSSIVNTSFNIHEEPIVCTPQDAIRAFQIGHLDLLAIGPFLAKNAGADQRVQHVRPV
                     AATND"
BASE COUNT         1557 a         3329 c         3220 g         1708 t
ORIGIN      
        1 tcacgaagcg ggagaccgac ccgcgtcgca ggtcggcgtc gatcaacagg gtctttactt
       61 cggggtcggc cgcaaacgcc agaccgagat tgatcgagat cgtcgacttc ccgtccttcg
      121 gcacggcgct cgacacgagc atcacctgcg ggcgaccgtc gggtaggttg gtcagcgtcg
      181 tcttcagccg gcggaacttc tccgcgtgga tcgaacgcgg ggcggccacc atcacgatct
      241 cgggccggtc gaccagccgg cgccggatca gcttggccag cggcactccc tcgacgacct
      301 tcttcgtacc caagcgacgt gcctcgcgat cgcgcgcccc gcggaaacgc gaaccgatat
      361 tgaacggctc gcggctcata ggacgtcctt caacttcaac ccgagcaccg taccgagcac
      421 ggcagaggac agcaacgaca gcgcgacgtt cctcaagagc agcagccgcc gccgccgggc
      481 gatcgtcggt gtcggaatcg acgggatcga aaccagaacc gggatcgccg ccgtctcgag
      541 gatccgcgct tcggagctga cgaccggggc gaggatcgtt cgccccaaca ccggcccgga
      601 gaacagcaag agcccgaccg cgatacccac gccgtagaac aagagcggac gcgggtggat
      661 cgggttcgac ggcggagcgg cgccggcgat gatctggaac tgatcgccct gcccctgttg
      721 ctcgaccaac tcggaggtgc cggccttgat cgcgttttct ctcagccggc ggtattccgc
      781 cgtcaagctg tcgatctcgt cgaccaactc ctcacgacga cgttcgaccc cgggtgtctc
      841 cgacaaccgt cgctggtact tcgcgatgtc cgagcgcagc gtctcggttt cggccttgat
      901 ccggttcagc tcggcgcgag cgctcgcgat ctgcacgtcc caggcgcgac tggtctcgct
      961 gcgcgccttc tcgccgtcgg cgaccggctc cgacggcgcg gtcgatcgct gcgactcctg
     1021 ctgcttctcg taccgctcga tctcggcctt tttcttgcgg acggcggggt gattctcgga
     1081 gtagtcgatc agcagctgct gcagctcgat ctcgagcacc gacaaaccgg cgtcaccgga
     1141 gccggcgacg gcccccggga ccgggacgaa cgaatccagc atgcccgctt gcgcttggtc
     1201 ggcttcgagg aagtcgatct gcagctgcag ctcggcctgg gccttaagat tcgcgtccag
     1261 tgcgaggcgg gcgtcttcga gagcctgctg gttgatcggt cgctgtgctt cgttctggta
     1321 cggattcccg gcgtcgaacg tctcgagctc gcgcttgagc cgctggatgt cggtccaacg
     1381 ctcggcggcg agcttctcga aggtctcggc ggtgaccgag gcgcggtcct ggcggaattt
     1441 cagattctcg tcgacgtaga acgtcgcgag caggttggcg atccgcgccg cacgataggg
     1501 gtcggcgtcc tggaaggcga tctggacgta atcctcccat cgcgggagcg tgacgcgaac
     1561 gcccgaccgg atgcgacccc tcagcgcgtc gcgctccttc tcgctcggcg attcgccgaa
     1621 ctcctccgtg atgatgcggt cgaggttccg ctcgctcatc acgttttgac ggatctcggt
     1681 cagcttggtc gccgggtcgc ccggcacggt atcggcgatc acgccgtcgg ggagctgctc
     1741 ggaggtgatg tcgagctgcg ccgccgcctc gtagaccttc ggcgtgaagt ggagcgcgac
     1801 gacggcgacc gacaggccga cgctgaatcc ggccacgacc gtccaccagt tccgggccag
     1861 gttgtcgatc agctcgaaca ggaccaaccg ctgttgattc gctttcgctt ccatttgggc
     1921 tcttgtacca tctcccgcat cgaccggcca tgcggactac gcctgcgcgc cggcgagcgt
     1981 cgcggaccga tcgacgagac gacaccccgc ggtggtcttc cggtccctga cccgaggtcc
     2041 cctatcttgc gtctggggct attgttacga acaccggcgt gtccggtcaa gcggaaagct
     2101 cgaacaggaa cgaaagggat cagatttctc gggacttcca cggtaggctg gcgtgcaact
     2161 gcccatctcc tcaacaactt gcatcgaccg atcggtcgcg gcaggctccg gtcccgcgcc
     2221 ggacggcctc gcccgcgcgt cttgacccgc ccgatgccga agcgtagctt cagcgggcaa
     2281 acgcattccg gatcgcgaat cagggggtcg gtgatcagct tcatccagac gcgagcgttt
     2341 gggtccacgc caatcctcgc gtgcctcttg gtctgtgcgt tcgcctccgg ctcgacgagc
     2401 gaagcccgcg tcacgttcga cccctcgctc tttctgaccg tcgagacgac cgacaacgtc
     2461 gccttcgcca actcgaccca gccgaagacg tccgactcca acgcgacgct cggggtgacg
     2521 ctccccgtcc ggcgcgagac cgcgcgcggt tggctcgagt tcctctactc gaccggcttc
     2581 tcgcgctacc gcgagatcac ggccttcgac gacatcaacc acctgttgga attccgcagc
     2641 taccgtgagc cgaacccggc cacgcggctg aacgccgagg cttgctacct gaggacccag
     2701 cagcagaacc gcggctaccg ggtccgatcg tgcaccgagc tgttcaccac cgagcggacg
     2761 cggcgcgagc tgttcggcgg tacggtcgac tacaaccgac gcctcggccg gttgtggtcg
     2821 tggaatgtcg aactacaggc ggcggtcgcc aacttcgatg cgatcgaaga cttcgatccc
     2881 ggagacaccg gtctcgtggt cgaggacaag gtcggctacc gggcgagcgc cggcatcgcc
     2941 cgccgattcg gtcggcgatt gcgcctcggc acgtcctaca gctacggcgt cgtcttccaa
     3001 gacctgtcgc ccgacgtcga gacgcaccgg ctgcgtctcg acgtcgagca cgaagcgacg
     3061 cggcggctgc aataccggct ccaggtcggc gcctactacc ggtcgtcgga cgaggtcgac
     3121 gagcagatgc aggacgacct tcgcacgagc ggagtcgagt tcctgttcga gctgcagtac
     3181 ggcgagtcga agggctaccg gatcgggccg gtggtgctcg agctgggtgc cggtatccaa
     3241 ccgaccgccg gcagcgggct gcagggcacg tcgaccgacg ccgtcgtcag tgcgggtctt
     3301 tcgccggttc ggtcgcagcg atggaactgg cagttcgagt cgacctacgg cgtgcgcatt
     3361 ccgaccgaaa gcgacgccgt cgatatccgc acgttgcgcg gcagcgcctg gatcgagcgc
     3421 gagctcgggc gggcgttcgg cgcgcggctg cgggggtatt tcagcgacca gatggccgac
     3481 gatccgacct acgccgtgtc gttgtacatc gtcgaaatca gcctcgcctg gtacttcctc
     3541 cgccacaggt agcgggaaca gtctgtgtca atctcgcccg tttcccgccg gatcgcccgg
     3601 ctcgagccgg ggtgcgcgat gcgtcgcgcg cgcaagctcg caccgaaaaa accgggtttg
     3661 gcccggaccg gggcccggcg aatcgggcgg gtggcacatg gcttgctgta aagtgtcctg
     3721 gctgaggagg aaccggcctt ttcttcgggt cggctcgcgg tcgagggtcg aggatgggat
     3781 gctgatcact cgagcacgtc tagtaccgct gtgcgtcgtc gcgctcgccg cgggcgcggg
     3841 ggcggcgcga gcccagtcgt cgtcctaccc gccgagcacc gtgattacgg atatttcgtt
     3901 cgacatgacg acgctgcaac agcacgcgcc gggaagcgac aactggatcg tcacctggtc
     3961 cgacgacggc caccaataca cttcgttcgg ggacgggggc ggcttcggcg ggaccgacaa
     4021 cgacggacgc gtctcgatgg gcgtcgcccg gatcgagggc ggcaaggaca gctacgtcgg
     4081 catcaacgtc aacggcggca agaacccgga gagcggcaac tcgacctggt ccagtaccgg
     4141 cggcaagagc ttggggatca tctcgatcga cctgtcgggc tccggcggcc agcggggaac
     4201 gctgttcatg taccgcaacg ggacgggctc cgaagccggc gcgttcgagc agacggagct
     4261 gtacaggtcg ctcgaccacg ggaggagctg gagctacacc ggcgtccgct ggaccgaccc
     4321 cggcttcttc tcaccgtcgt tcctgcagtt cgggcaggac tacgccggcg ctcgcgacga
     4381 gtacgtctac agctacgcac cgaccaacca gaacacgacg tgggaggtgc agccggccgg
     4441 ggagatcctg ctgttccgcg tgcgcaagga ccagctcgcc tcgcccgggg cgtgggagta
     4501 cttctccggt acggcctcga acccgtcgtg gagctcgaac ctgtcgaacg cgacgccggt
     4561 attcgccgac ccggccaacg gcgtgatgcg gacgagcgtc agctacaacg cgcctctcgg
     4621 ccggtacatc ctcatcacgc agcaggtcag tcgattcgag gccgacgact accacatcgg
     4681 catctacgac gccccgcagc cgtggggacc gtggacgacg gtcttcttcg gcaacccgag
     4741 ggtcgacctg agccccagcc tgaacagcgg ctcgaagacc gtgttctgga acctgtcgaa
     4801 caagtggctc agcgccgacg gtcgcgattt cgtcatggtc tacaccggcc cgggcccgga
     4861 caacatgggg acggtcgagg gccgcttcgt cacggtgacc gacacgtcgc cgccgagcgc
     4921 accgcagaat ctggtggcga cggggtcgag cgacgactcg atcgggctga gctggtcggc
     4981 gtcgtccgac ccgcagtccg gaatcagcca ctaccggatc cgccgcgacg gtacgaccgt
     5041 cgctcagccg cccggcacga ccttcgtcga caccggactg gccgaggcga cggcctacac
     5101 ctaccaggtc agcgcggtca acggggccgg gctcgagtcg accgcggcga cgaccgtcgc
     5161 atcgacgctc gccgatctga cccctcccgc gctgacgtcg gtcgtggccg gcgggccgaa
     5221 ccaggtcacc gtcaccttca gcgagcccgt cgaggtcctg accgcaacca acgcggcgaa
     5281 ctaccagatc gacaacggga tcgccgtcgc cggcgccgcg ctcgacgccg cggggctcgt
     5341 cgtcacgctg cagacgtcgt cgcacggcgt cggcccgacc tacacgctga cggtcaacgg
     5401 cgtacgcgat cgcgcggccg cgccgaacac gatcgcgagc aacacgcagg cgagctacac
     5461 cttcgccgat cagctgacga tctcgtcgat cagcgttgcc tccggcgagc cgtacgtcgt
     5521 cgcgcaaccg ctgagcaacg gctcgttggc ctatatcgac cggaccttcg gttacagcaa
     5581 tgtcccgcag ttcctgctcg gcgcgcagta catccgcacc gcgaacgacg acaagcagag
     5641 ccagggaagc gcgtttctct cgtttcaggt caaccgcgcg gtcaaggtct acgtcgcgca
     5701 cgacgatcgc tacgggacga agccctcgtg gctgcagagc ttcctcgaca ccggaatcgg
     5761 catctcgctc gacgtgtcgt tcacgttgtt cgagcagccg ttcccggccg gaacggtcac
     5821 gctcggcggc aacatcgaac ctcccgagac gcaggggtac tcgatgtaca ccgtgatcgt
     5881 cgtcgccgac ccgggcggag acaccgctcc cgccccgccg acgggcgtcg acctcaacta
     5941 gcctgaccga gtggacgcgg ctccccgact gagctaagtt ttcggctcgg ccgccgcgga
     6001 acgggcggtt ggctcctacg gggtgccgtg tgtcgccgag cctctcagcg caggctggcc
     6061 ggactgccga acgcggccgg ggccgaaccc gaagtcgcgt caagctcgcg gtcaaggcgc
     6121 tggtcaccct cggcctgctc gcgttgttgc tcgaccgggt gaacctcgac gacagcgtcc
     6181 gagcgctgcg acgcgcccag ccggtttgga tcgtgctggg cacgttggtc tgttccaccg
     6241 gattcctcgc gtcgagccag aagtggcacg gcttgctcgc ggcgatgggc gtagcgatcg
     6301 accgctgggc ggcgctgcgg atctacgcca tcggtcactt cgccagctcg ttctttccgg
     6361 gcacgatcgg cggcgacctg ctgcgatgca acatggtgcg gccggtgacc ggcgggtacg
     6421 ttcgcgcctt cgccagcgtg ctcgccgagc gagcctcggg cgtggtcacg atggtcgcgc
     6481 tcgcgatcgc ggccgtcgcc tggagacccg atcgactcgc cacgccgccg gtgctgacgc
     6541 tggtcggcat cggcagcgcc ggcgtcgtgc tcgcgttgct gctcgcgctc aatcgacggt
     6601 tggccgtcgc ggtcacctac cgcctgcgcc gtggctcgtt gcggcgcgtc gttcgacgac
     6661 tgtacgagct gcatcggatc ctccggtcgt tgtcggcgtc ggccctggcg gtcgccctcg
     6721 gctggtcggt ggggttctat ttcggctccg ggctgttgat gtacacctgc tgccgtgcct
     6781 tcgagcgccc gatcggagtg ctcgacgcgg tatcggtcgt cgtcgtagta tcgatcctga
     6841 tgttgatccc gatcagcctc gggggcttgg gtcttcggca ggtcggcgac gtgatgatgc
     6901 tcgacctgtt cggtgtcgat ccggggtcgg cgctcgtcat ctccctgttg cggcaggcga
     6961 tcaactactt ctacacgttg atcgggggcg tgttcttcgt gctctggacc ggtgccgagc
     7021 tgggcccgca tgtttcacct cgcgaagagg gactgtgatg ttcgaccgcg cgagctcggc
     7081 gttctccgcg ctcaaaggcc ccgaaccgtt cgacgcctgc gtcatcggct cgggtttcgc
     7141 cggcatcgtc gtcgccaagg ggctggtcga gcgcggcgcc cgtacgctgc tgctggaatc
     7201 cggccagggc atgctgaact ggctgctcga caagcggctg cacgagctgg cggcgtacga
     7261 gttcagcggc gacaccgact atccgctcaa acggaccaag gcgcggatcg tcggcggcaa
     7321 ctcgaacttc tggaccggcc gcgccgagcg gttccacccg tcggatttcg ctgaccatcc
     7381 gtacctgccc agcccgtggc cgatcggcta cgacgagctc gagccctact acgtgcgggc
     7441 cgaacgcacg ttgcgcgtgc gcgccgccga cttcagcgag ttcgccgcgc cccgctcgga
     7501 aaacccggcc gttccgccgc gtccgaacat ctcggacttg cgcgcgatcc tcgcgcgcgc
     7561 cggcgtcacg ctcgattact cggcaaccgc gacgccgcgg cgagcgtttc gcttcttccg
     7621 gatgaaccac gaggtgttgc cggaggtcag gcggaatccg cgcctcacgc tcgtgtcgaa
     7681 cgcgaccgtc actcgcttgc tcgtcgagcc ggacgggctg gtcggcgggg ccgaggtgcg
     7741 cgcgctcgat cgtacgacca aggtcgctcg ggccaaggtc ttcgtcgtct gttgcggcgg
     7801 gttcgaatca ccgcggttgt tgctgctttc gcgctccgag tctttcccga acggcatcgg
     7861 caaccggttc gatcgggtcg gacggggttt caacgagcac gccagcgtca acttctacgg
     7921 cgcaatgacc caccggcgcg gcaccgtgct gccacgacac aagatcggcc gcagccatca
     7981 gttctacgag cggtaccgca acgaagggct cggtgcgttg ttgctcaacg cgatccaatc
     8041 gtgggtcttc cccaaccacc tgctcaagtt ccgcctggtc gacatgccgc gctacgcggc
     8101 attcctcgcc aagcgggtga tccgacccga gctctatctc ggaacgacga tcgagatgac
     8161 cgtcgaggac tcgaaccgca tcacgctctc cgacaggatg acggaccact tcggcaatcc
     8221 gctggcgcac atgatcttca actactcgac ccaggaccgg ctgctgatgg agcgctcccg
     8281 cgagctggtt cgcgagatct actccaagct cggcatcagc gagatccgcg aagggccgga
     8341 caatttctcg atgcatcacc agggaacgtg ccggatgggc gacgacccga aggtcagcgt
     8401 ggtcgaccgc gacctccggg ttcacgaatc accgaacctc tacgtctgcg gcgcggagaa
     8461 tctggtctcc ggcagcgcgg tgccgccgac gctcacgctg gtcgcgctgg cccaccggct
     8521 cgccgaccac ctcggggaag cgttgcaccg gctcccgagc cggtcgtacg ccgcgcgaga
     8581 gtccggccga ccttgagcta cggccgggcg caggccgtct agtcgttcgt cgccgcgacc
     8641 gggcggacgt gctggacccg ttgatcggcg ccggcgttct tcgccagaaa cggcccgatc
     8701 gccagcaggt cgaggtggcc gatctggaac gcccggatcg cgtcctgcgg cgtgcagacg
     8761 atcggctctt cgtggatgtt gaagctggtg ttgacgatgc tggacaagcc ggtcagccac
     8821 ttgaactcgg tgaggatgcg gtagtagctc tcgttgtcgc tcctcgccac caactggggt
     8881 cgcgccgtat tgtcgacgtg aaccacgccc gagcaacgct cggccatctc cttcgtgcaa
     8941 tcgaaggtga tcgtcatgta acgcgcggtg tcggtcgccc cggggaggtt cttgaacgac
     9001 aaatgcgcgt cctcggtgat cgtcgacggc gcgaacggca tgaactcggt gcgcttcagc
     9061 cgctcgttca gccagtcgtt gaccgacgga tcgtcggggc ggtagaggat cgagcggttg
     9121 cccagcgccc gcggaccgta ctccatcgcg ccgtggaatc gcgcgaccac gtagccgcgg
     9181 ttgatcaacc gggcgatctc gccctcgacg tcgtcgtggt gcgtgtaatc cactccggcc
     9241 gcgctcagcg cgtcgccgat ctcccgatcg gagaacgacg gaccgaggta gacgtggtcg
     9301 atgcacttct tgccggccat cgccagcccg gggcgcttgt ccgagcagag cagcagcgcc
     9361 gcaccggccg ccaggccctc gtcggacatc gccggaaaga cgaagaccga ttcggctccc
     9421 gggatctcgt ggatgcgctg gttgatcttg acgttggcca ccacgccgcc ggcgagcgcg
     9481 atcttggtct tgccggtctt cttggcccag taggcgacat attgactgca gatctcctcc
     9541 gacaggtcct ggatcgaggc ggacaggtcg gccttgtcga aatctccggg cagcgcccgc
     9601 ttcagcttct ggaccgcagc cccgcggaac gcgttccccg tgttccgcat cgtgccgtgc
     9661 tcgtaacgga tgaaccgctc gaggacgggc ttgtagcgcg gctttccgtg tgcggccagg
     9721 ccggtgatct tgccctcgtg cttgcccatc ttgtagccgc acaggtgggt cacgtagccg
     9781 tagtagttgc ccagcgagtc gaacgagggg atcg
//
LOCUS       DASPGN010000154         3975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1313020, whole genome shotgun sequence.
ACCESSION   DASPGN010000154 DASPGN010000000
VERSION     DASPGN010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313020"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /gene="hisS"
                     /locus_tag="VD788_04295"
     CDS             <1..1020
                     /gene="hisS"
                     /locus_tag="VD788_04295"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXV75516.1"
                     /translation="NTASVARAFVEHSLHRRVSAGFPERLYYIGPMFRHERPQKGRQR
                     QFHQVGAEVLGAAEPIVDAEMMQMLDLFLDTLGVGGRELLIASVGDAACRPAYREILR
                     EWLEPRLGSLCDDCRRRHADNPLRVLDCKVERDRAILAEAPLLADHLCRDCAGHFAEV
                     ERLLERYGLRYRVDPRLVRGLDYYQRTVFEVIGGGLGAQDAILGGGRYDGLVEQLGGP
                     SMPAIGFAMGIERALLAMPDGSVESPGIDLLLVALGDDGWRAAVELASELRRNGVRVL
                     YSTCPRPMGAQLKRADRAGARYALFIGADELAAGSFALKDLRTGRQQSMTLEQVITRI
                     GDDHA"
     gene            1013..2794
                     /gene="aspS"
                     /locus_tag="VD788_04300"
     CDS             1013..2794
                     /gene="aspS"
                     /locus_tag="VD788_04300"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXV75517.1"
                     /translation="MPESNDGLLKRTVGCGELGEADAAARREVVLTGWIHRRRDLGRL
                     IFVEVRDPTGTVQVVFDPSHAQPAHAVAESLRAEFVVGVRGVVIVRESPNPKHPSGTV
                     EVRADEIVIHNRADHLPIPVADEGETNEEARLTHRYIDLRRPAMQYALRLRHRLAVAA
                     RGVLDRHGFTEIETPMLTRSTPEGARDYLVPSRVQPGSFYALPQSPQLFKQLLMIAGY
                     DRYFQFARCFRDEDLRADRQPEFTQIDIEMSFVRRDDVYVVVEELLREMFARIGVTIE
                     PPFRRMSYYEAMDRFGTDRPDLRFGMELRDPGESVRDTGFRVFEETLAAGGAIRGLAV
                     PGGADVPRKQIDEWTDYVRRLGARGLVWIKLEQGTVGGSVKKALGDARCRAIATAVGA
                     SDGDLALLVADTTEVARSALGALRLRVAADRGAIPSDRWELLWVEQFPLFGWDAGQRR
                     HVAIHHPFTAPEWDQLALMESDPAAVRSQAYDVVLNGTELGGGSIRIHRRDVQDRVFR
                     ALGIDEEEARAKFGFLLQALDSGAPPHGGIALGFDRICALMAGHESIREVIAFPKTTR
                     ASCLMTGSPSAVDPRQLRELGITLVRD"
     gene            2901..3761
                     /locus_tag="VD788_04305"
     CDS             2901..3761
                     /locus_tag="VD788_04305"
                     /inference="COORDINATES: protein motif:HMM:NF025704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HXV75518.1"
                     /translation="MFREKRSLFVRSSWFVALLLLVATGCSKKTPQQVIDVNQALAAA
                     KDDCATVYATNDLNAIQQRVDGMNALVDDKKMGKAKKEAVPLLPEVEQLGQTAEASRA
                     HAKTAADSAIAAATKAVQEARDAETATYNPDGFRQAESKLNQATAAMNDPCRYNEAKQ
                     LAEDASRQAANAREAAMAEKRRQEEERRRAEEEARLAALEAQRQEEERKKNMVPPTYV
                     VEPGNSLWRIAGMQRIYDNPGFWPIIHDANSGKIANPDLIYPGQELTIPRDYSDLEML
                     DLLYRLWSNY"
BASE COUNT          675 a         1284 c         1386 g          630 t
ORIGIN      
        1 aacaccgcgt cggtcgcgcg cgcgttcgtc gagcactcgc tccaccgccg cgtcagcgcc
       61 ggctttccgg agcggttgta ctacatcgga ccgatgttcc gtcacgagcg gccgcagaag
      121 ggcaggcagc ggcagttcca ccaggtggga gccgaggtgc tcggcgccgc cgagccgatc
      181 gtcgacgccg aaatgatgca gatgctcgat ctgttcctcg acaccctcgg cgtcggcggc
      241 cgcgagctgt tgatcgcgtc ggtcggggat gccgcttgcc gtccggccta tcgcgagatt
      301 ctgcgcgagt ggctcgaacc gcggctcgga tcgctgtgcg acgattgccg gcggcgccac
      361 gccgacaacc cgctgcgggt gctcgactgc aaggtcgagc gggaccgcgc gatcctcgcc
      421 gaagcccccc tgctcgccga tcacctgtgc cgggactgcg ccggacactt cgccgaggta
      481 gagcggctgc tcgagcgata cgggctgcgc taccgtgtcg atccgcggtt ggtgcgcggc
      541 ctcgactatt accagcgaac cgtcttcgaa gtgatcggcg gtgggctcgg cgcacaagac
      601 gcgatcttgg gcgggggtcg ttacgacggg ctcgtcgagc agctcggcgg tccctcgatg
      661 ccggcgatcg gtttcgcgat gggcatcgag cgcgccttgc tggcgatgcc cgacggctcg
      721 gtcgagtcgc ccggcatcga cctgctgctc gtcgccctcg gcgacgacgg ctggcgcgcg
      781 gcggtcgaac tggcgagcga gctgcgccgg aacggggtcc gcgtgctgta ctccacctgt
      841 ccgcgaccga tgggagcgca gctcaaacgg gccgaccgcg cgggagcaag gtacgcgctg
      901 ttcatcggcg cggacgagct cgccgccggg agcttcgccc tgaaggacct acggaccggt
      961 cgccaacaat cgatgaccct cgagcaagtc atcacgcgta tcggagacga tcatgcctga
     1021 gtcgaacgac ggcttgttga agcggacggt cgggtgcggc gagctcggcg aggcggatgc
     1081 cgccgcgcgt cgagaggtgg tgctgaccgg gtggatccac cgtcggcggg acctcggccg
     1141 gctgatcttc gtcgaggtgc gcgacccgac cggtaccgtg caggtcgtct tcgacccgtc
     1201 gcacgcgcag ccggcccacg ccgtcgccga gtcgttacgc gcggagttcg tcgtcggcgt
     1261 tcgcggtgtc gtcatcgtac gggagtcacc gaacccgaag cacccgagcg ggaccgtcga
     1321 ggtgcgcgcc gacgagattg tcatccacaa ccgcgccgac catctaccga ttccggtcgc
     1381 cgacgaggga gagacgaacg aggaagcccg gctgacccac cgctacatcg atctgcgccg
     1441 accggcgatg cagtacgccc tccggttgcg gcatcggctt gccgtcgcgg cacgcggcgt
     1501 attggatcgg cacggcttta ccgagatcga aacgccgatg ttgacccgct cgacgccgga
     1561 gggtgcgcgc gactacctcg ttccgtcgcg ggtccagccc gggagcttct acgccctacc
     1621 gcagtcaccg cagctgttca agcagctgct gatgatcgcg ggctacgacc gctacttcca
     1681 gttcgcacgc tgcttccgcg acgaggatct gcgggccgat cgacaacccg agttcacgca
     1741 gatcgacatc gagatgtcgt tcgttcgccg tgacgacgtc tacgtcgtcg tcgaggagct
     1801 gttgcgcgag atgttcgctc ggatcggtgt gacgatcgag cccccgtttc gtcggatgag
     1861 ctactacgag gcgatggatc ggttcggcac cgaccggccg gacctgcgct tcggcatgga
     1921 gctgcgtgat ccgggggagt cggtacgcga caccggcttt cgcgtattcg aggagacgct
     1981 ggcggccggt ggagcgattc gcggtctggc cgttcccggg ggtgccgacg tcccgcgcaa
     2041 gcagatcgac gaatggaccg actacgtccg ccgtcttggg gcccgaggat tggtctggat
     2101 caagctcgag caggggacgg tcggcgggtc ggtcaagaag gcgctgggcg acgcgcgttg
     2161 tcgcgcgatc gcgacggcgg tcggcgcgag cgacggtgat ctggcgctgc tcgtcgccga
     2221 cacgaccgag gtcgcgcgga gtgcgctggg cgcgctccgg ctgcgggtcg ccgcggaccg
     2281 cggggcgatc ccgtccgacc gctgggagct gctgtgggtc gagcagtttc cgctgttcgg
     2341 ctgggacgcg gggcagcggc gacacgtcgc gatccatcac ccgttcaccg cgcccgagtg
     2401 ggatcagctc gcgctgatgg agagcgaccc ggcggcggtt cgctcgcagg cgtacgacgt
     2461 cgtgctgaac gggacggagc tcggcggggg gagcatccgc atccatcgtc gtgacgtgca
     2521 ggatcgcgtc tttcgcgcgc tcggcatcga cgaggaagag gcgcgagcca agttcggctt
     2581 cctgctgcag gcgctcgact cgggcgcgcc accccacggt ggcatcgccc tcggattcga
     2641 tcggatctgt gcactgatgg ccggccatga gtcgatccgc gaggtgatcg cctttcccaa
     2701 gacgacacgt gcctcgtgcc tgatgacggg ctcgccgtcc gcggtcgatc cgcgccagct
     2761 tcgagagctc ggcatcaccc tggtccgcga ttgacaaacc atcgagaatc atcgggttgc
     2821 ggccacgttc gttgaccctc gagaatgccg tgtgctagcg tggccggcga gaacaccgat
     2881 cgctcgagga ggaggccaag atgtttcgcg aaaagcgcag tcttttcgtt cgttcaagct
     2941 ggttcgttgc gctgttgttg ctcgtggcaa ccggatgttc caagaagacc ccgcagcaag
     3001 tcatcgacgt caaccaggcg ctcgccgcag ccaaggacga ttgcgcgacc gtctatgcca
     3061 cgaacgatct gaacgcgatt caacaacgcg tcgacgggat gaacgccctc gtcgacgaca
     3121 agaagatggg taaggccaag aaagaagccg tgccgttgct gccggaggtc gagcagctcg
     3181 gccagacggc ggaggcgtca cgagcccacg ccaagacggc agcggacagc gcgatcgccg
     3241 ccgcgaccaa ggccgtgcag gaagcccggg acgcggagac ggcgacctac aacccggacg
     3301 ggttccggca ggccgagtcc aagctgaacc aggcgacggc cgcaatgaac gacccctgcc
     3361 gctacaacga ggccaagcag ctcgccgagg acgcctcgcg ccaggccgcg aacgcgcgcg
     3421 aggcggcgat ggccgagaaa cgccgccagg aagaagaacg caggcgggcc gaggaagaag
     3481 cccggctggc cgcgctcgag gcgcagcggc aggaggaaga gcggaagaag aacatggttc
     3541 cgccgacgta tgtcgtcgaa ccggggaact cgctgtggcg catcgccgga atgcagcgca
     3601 tttacgacaa tccgggtttc tggccgatca tccacgacgc caacagcggc aagatcgcga
     3661 atcccgactt gatttacccc gggcaagagc tcaccattcc cagggactac tccgacctgg
     3721 aaatgttgga tttgctctat cgactctgga gcaactattg actcggagcg atagcgcgaa
     3781 ggagtgaatg ggttgcgcga cgccggcgtg acggggtgaa tgcccgccag accggccgag
     3841 gcggtagctc gactgccgcc cgcgcgcccg acccatgaag acgctgacag tcgacccgca
     3901 ggtcatcgag gcgatcgccg cgcatttcga cgaggcattg cgcctgatcg agaagcagtt
     3961 tcgggtcagc atctc
//
LOCUS       DASPGN010000155         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1079910, whole genome shotgun sequence.
ACCESSION   DASPGN010000155 DASPGN010000000
VERSION     DASPGN010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079910"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VD788_04310"
     CDS             complement(<1..921)
                     /locus_tag="VD788_04310"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXV75519.1"
                     /translation="MDDLLRRAAALGFGGQVVVERDGELILARAYGQADRRAGRPFAI
                     DTPVGIASISKHLTALAVLRLVDRGRVRLSDPIERHLDSVPPDKLGITIEQLLTHTSG
                     LPGGDAAGDFELDSRAELLERILALDRAAASGERWIYSNSGYSLLAAIVEEAAGIPYE
                     QFVDEQLFAAAGMRHSGFWHRPPPDGLPAAHPYIGWRDQGSPADWPRNWRVFGAGDVL
                     STAADLYRLDRALRRGELVSRRLWRAARSVQAEIDETTGYGYGLFVYTDAAGRTVLEH
                     GGDWQRGYNGLFMRYVDEEIVIVVTSNARDH"
     gene            complement(1100..1660)
                     /locus_tag="VD788_04315"
     CDS             complement(1100..1660)
                     /locus_tag="VD788_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXV75520.1"
                     /translation="MTRWRFPAVAIICVLGGVGAARCSDAGRACDGQASTTVLLVRHA
                     EKSAEPAEDPRLSPVGETRAAELVRVAGEAGIDAIYVTATTRTRRTAEDLAAHLGLTP
                     IELAPADTDGLARRILDRHRGQTVLAVGHSNTVPAVIEALGGPSLPDIDEADYDDLFV
                     VTVPCAGKVTQVQLAFGVASPRSGPP"
     gene            1812..>2038
                     /locus_tag="VD788_04320"
     CDS             1812..>2038
                     /locus_tag="VD788_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75521.1"
                     /translation="MKRSFVLLAALSLAAPIAAAERLIAFTGATVIPIAGPSIDDGVV
                     VVRGKTIEAVGRAEEVRVPAGAERIDLAGRVL"
BASE COUNT          297 a          704 c          733 g          304 t
ORIGIN      
        1 gtggtcgcgc gcgttcgagg tgacgacgat cacgatctcc tcgtcgacgt agcgcatgaa
       61 caagccgttg tatccgcgtt gccagtcgcc gccgtgctcc agcaccgtcc ggcccgcggc
      121 gtcggtgtag acgaacaggc cgtagccgta gccggtcgtc tcgtcgatct cggcctgcac
      181 cgatcgggcg gcgcgccaca gccggcggga gaccaactcg ccgcgtcgca atgctcggtc
      241 caaccggtag agatcggccg ccgtcgagag gacatcgccc gctccgaaga cgcgccaatt
      301 gcgcggccag tcggccggcg atccctggtc gcgccagccg atgtaggggt gcgccgcggg
      361 cagaccgtcc ggcggcgggc ggtgccagaa accgctgtgc cgcatgccgg ccgccgcgaa
      421 caactgctcg tcgacgaact gctcgtaggg aatacccgcc gcctcctcga cgatcgcggc
      481 cagcagcgag tatccgctgt tcgagtagat ccagcgctca cccgacgccg cggcccggtc
      541 gagcgcgagg atccgctcga gcagctcggc tcggctgtcg agctcgaagt cgccggcggc
      601 gtcgccgccg ggcaggccgc tcgtgtgcgt cagcagctgt tcgatcgtga ttccgagctt
      661 gtcgggtggc acgctgtcga gatgccgctc gatcgggtcg gacaaccgga ctcgcccgcg
      721 gtcgacgagg cgcagcacgg ccagggcggt cagatgcttg ctgatcgagg cgatgccgac
      781 gggcgtgtcg atcgcgaacg gccgccccgc gcgccggtcg gcttggccgt aggcgcgggc
      841 gaggatcagc tcgccgtcgc gctcgaccac gacctggccg ccgaagccga gcgccgccgc
      901 acgccggaga agatcgtcca tccgccgtgc gacgggcccg gcgacgatct cggggagggg
      961 cgccgcgggc gtttcttcgg ccgcgacgcc ggatccgcct gcggaagcga ggacgaggag
     1021 cgtgagcgag aaaattcgca tagtgcgaaa ataatcgcat gcagaccgtc gcctgtcaag
     1081 cggaaaacgc ggggcgcgat cacggcgggc cggaccgggg tgaggcgacg ccgaaggcga
     1141 gctgcacctg cgtcaccttg ccggcgcacg gcacggtcac gacgaacagg tcgtcgtaat
     1201 cggcttcgtc gatatccggc agcgacggac cgcccaatgc ctcgatcacg gccgggacgg
     1261 tgttgctgtg acccaccgcg agcaccgttt gcccccggtg ccggtcgagg atccgacgtg
     1321 ccaggccgtc ggtgtcggcc ggggccagct cgatcggggt cagaccgagg tgcgcggcca
     1381 ggtcctcggc ggttcgtcgg gttcgcgtcg ttgcggtcac gtagatcgca tcgattcccg
     1441 cctcgccggc gacgcggacc agctcggccg cccgcgtctc tcccaccggg ctcagcctcg
     1501 ggtcttccgc gggctcggcc gatttctccg cgtgcctcac caacagcacc gtcgtgctgg
     1561 cctgcccgtc gcacgcacga ccggcgtcgc tgcagcgggc cgccccgact ccaccgagta
     1621 cgcagatgat cgccactgcc gggaatcgcc accgtgtcat gtcgtgcctc ctcgatcggg
     1681 tctcggcccg acccggatta gagcagagcc gggcggagat tgtaagcacc ggcgcgaatc
     1741 gcggttggcc tcgacggccg gttcggtgta gcttcgggga ctcgattcgc gccgaccgag
     1801 gaggggtctc ggtgaaacga tcgttcgtgt tgctcgccgc cctgtcgctg gcggcgccga
     1861 tcgccgccgc agaacgcctg atcgcgttca ccggagcgac ggtgatcccg atcgcgggac
     1921 cgtcgatcga cgacggcgtg gtcgtcgtcc gtggcaagac gatcgaggcg gtcggccgag
     1981 cggaggaggt tcgcgtcccg gctggcgcgg agaggatcga cctggcgggc cgcgtgct
//
LOCUS       DASPGN010000156         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_25803, whole genome shotgun sequence.
ACCESSION   DASPGN010000156 DASPGN010000000
VERSION     DASPGN010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25803"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /gene="rph"
                     /locus_tag="VD788_04325"
     CDS             complement(<1..536)
                     /gene="rph"
                     /locus_tag="VD788_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXV75522.1"
                     /translation="MARHDGRTDRDLREIKLAPGYLDHPLGSVMIEMGRTRVLCTASL
                     EERVPKFLHGSGAGWVTGEYSMIPAATDTRRPREIGRGRPSGRTMEIQRLIGRAMRSV
                     VDRRALGERTLWVDCEVLQADGGTRTASVTGGFLALCLALAAMRKNKQLASPVLSGMV
                     AAVSVGIVEGRPMLDLDYV"
     gene            complement(545..1399)
                     /gene="murI"
                     /locus_tag="VD788_04330"
     CDS             complement(545..1399)
                     /gene="murI"
                     /locus_tag="VD788_04330"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXV75523.1"
                     /translation="MPPHDDRPIGVFDSGVGGLTVFHALEQALPRETMVYLGDTARVP
                     YGGRSAETVRRYTREAARFMLRQQVKMVVVACNTASSVALDSMEHEAGLPVIGVIVPG
                     AARAVDASPTGRIGVIGTRATVGSGAYKRAIQELRPDAEVLSQACPLFVPLAEEGWTD
                     GEITRRIAESYLRPFQRAEVDTLVLGCTHYPLLRGVIGEVMGRGVTLVDSAESVADEA
                     ARRLAAAPELAGSAAAARGARDEGREHHFYVTDAPETFQVVAERFLGRPIRCLEWAAL
                     DRPSNGAG"
     gene            complement(1404..2051)
                     /locus_tag="VD788_04335"
     CDS             complement(1404..2051)
                     /locus_tag="VD788_04335"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HXV75524.1"
                     /translation="MTRALPRRPAVSLVALVALVALVCPAGCGGGGDDRERTGEEGGV
                     EPPGDPEGEATITPLARRSVEIYFPSTTDDGLVSEFREIFDTATPGDQIKQIVADLIS
                     GPTTDEATKALPGGTRLRQVYVLDDGTAWLDFSSELTDGIGGGSMKELLVVYAVVNSV
                     VANVPQVRKVGILVSGRPVETLNGHVHLLNPLTPNFSLILGSTTVARRHPAATRD"
     gene            complement(2048..3226)
                     /locus_tag="VD788_04340"
     CDS             complement(2048..3226)
                     /locus_tag="VD788_04340"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXV75525.1"
                     /translation="MIEHAPQLRRVALALLLLGAAAATPAAAQRASVQIKVDSSESSM
                     RLVLTHSSSEAYEVRQQDGLIEVIYATPVRAEPSELLLDDPILSRFRMEDDRRLVLAT
                     GPGFRSFETFELRNPFRLIIDLQGSRGGEEAPLPVERPRGRKGTRGTVIVLDPGHGGV
                     ELGAQGPTGLEEKDVALDLARRLERILRNDRKLTVVLTRDEDRLVGLDERTAIANHNR
                     ADLFVSIHLNASSRSNAQGAETYFLSSDASDDEARTLAALENRAAGVPTESVVTVDGE
                     RRNVELVLWDLAQNQYMAESSVLAENMQIHLNELTGTRDRGVRQAPFRVLMGATMPAI
                     LVEAGFISNPDEEERLRSSDYRNRVAQAMANAIYDFLAELERLSRPGASAGGGGSSRP
                     "
BASE COUNT          520 a         1289 c         1173 g          496 t
ORIGIN      
        1 acgtagtcga ggtcgagcat cgggcgaccc tcgacgatgc cgaccgagac cgcggcgacc
       61 atgcccgaca gcaccggcga cgcgagctgc ttgttcttgc gcatcgcggc cagcgcgaga
      121 cacagcgcga gaaagccgcc ggtgacgctc gccgtgcgcg tgccgccgtc ggcctgcagc
      181 acctcgcaat cgacccacaa cgtgcgctcg cccaacgccc ggcggtcgac gacgctgcgc
      241 atcgcgcgcc cgatcagccg ctggatctcc atcgtccggc ccgaaggacg accgcggccg
      301 atctcgcgcg ggcgccgcgt gtcggtcgcc gcggggatca tcgagtactc gccggtgacc
      361 caccccgcgc ccgagccgtg caggaacttg ggcacccgct cctcgagcga ggccgtgcac
      421 agcacgcgcg tgcggcccat ctcgatcatc accgagccga gcggatggtc gagatagccg
      481 ggcgcaagct tgatctcgcg caggtcccgg tcggtccgtc cgtcgtgccg cgccatgccg
      541 cccctcaccc cgccccgttc gacgggcggt cgagggccgc ccactcgaga caacggatcg
      601 gtcgtccgag aaaacgctcc gccacgacct ggaaggtctc gggagcgtcg gtcacataga
      661 aatgatgctc gcgcccttcg tcgcgggctc cgcgcgcggc ggccgccgac cccgccagct
      721 cgggcgctgc ggccagccga cgtgccgcct cgtcggccac cgactcggcc gagtcgacca
      781 gcgtcacgcc gcggcccatc acctcgccga tcacgccgcg caacagcggg tagtgcgtgc
      841 accccagcac caacgtatcg acctcggccc gctggaacgg cctcaggtag ctctcggcga
      901 tgcgccgcgt gatctcgccg tcggtccaac cttcctcggc cagcggcacg aacaacggac
      961 aggcctggct caatacctcg gcatccgggc gcagctcttg gatcgcacgc ttgtacgcgc
     1021 cgctgccgac ggtcgcgcgc gtgccgatca cgccgatgcg gccggtcggc gaggcgtcga
     1081 ccgcgcgagc ggcgcccggt acgatcacgc cgatcaccgg caggcccgcc tcgtgctcca
     1141 tcgaatcgag cgccaccgac gacgccgtgt tgcaggcgac gacgaccatc ttcacctgct
     1201 ggcgcagcat gaaccgcgcc gcctcgcgcg tgtaacgccg tacggtctcg gccgaccggc
     1261 cgccgtacgg cacgcgcgcg gtgtcgccga ggtagaccat cgtctcgcgc ggcaacgcct
     1321 gctcgagggc gtggaacacc gtcagcccgc ccacgcccga gtcgaacacc ccgatcggcc
     1381 ggtcgtcgtg cggtggcacc ggctcagtcc cgcgtcgcgg ccggatggcg ccgcgcgacc
     1441 gtggtcgagc cgagaatcaa cgagaagttc ggcgtcagcg ggttcaacag gtggacatga
     1501 ccgttcagcg tttcgaccgg ccggcccgag acgaggattc cgaccttccg cacctgtggc
     1561 acgttcgcca ccaccgagtt gacgacggcg tagacgacga gcagctcttt catgctgccg
     1621 ccgccgatgc cgtcggtcag ctccgaggaa aaatcgagcc acgccgtgcc gtcgtcgagc
     1681 acgtagacct ggcgcaggcg cgtcccgccg ggcagggcct tcgtggcctc gtcggtcgtc
     1741 ggcccgctaa tcagatcggc cacgatctgc ttgatctggt cgccgggcgt cgcggtgtcg
     1801 aagatctcgc ggaactcgct caccagaccg tcgtcggtcg tcgacggaaa gtagatctcg
     1861 acgctgcgac gcgcgagcgg ggtgatcgtc gcctcgccct cggggtcgcc cggcggctcg
     1921 acgccacctt cctcgcccgt tcgctctcga tcgtcgccgc ccccgccgca gcccgccgga
     1981 cagaccagcg cgaccagcgc gaccagcgcg acgagcgaca ccgccggtcg ccggggaagg
     2041 gctcgcgtca cggccggctc gagccaccgc cgccggccga cgcgcccggt cgcgacagac
     2101 gctcgagctc ggcgagaaag tcgtagatcg cgttggccat cgcctgcgcg acgcggttgc
     2161 ggtagtcgct cgagcgcagc cgctcctctt cgtcggggtt cgagatgaaa ccggcctcga
     2221 ccaggatcgc cggcatcgtc gcgcccatca gcacgcggaa cggggcctgg cgcaccccgc
     2281 gatcgcgcgt gccggtcagc tcgttgaggt ggatctgcat gttttcggcc agcacgctgc
     2341 tctcggccat gtactggttc tgcgccaagt cccacaacac cagctcgacg ttgcggcgct
     2401 cgccatcgac cgtgacgacc gactccgtgg gaacgccggc ggcgcggttc tcgagtgccg
     2461 ccagcgtgcg cgcctcgtcg tcgctcgcat cggaggacaa gaagtaggtc tccgcgccct
     2521 gggcgttgct gcgcgacgac gcgttcaggt ggatcgacac gaacaggtcg gcccggttgt
     2581 ggttggcgat cgccgtgcgt tcgtcgaggc cgaccaggcg gtcctcgtcg cgcgtcagca
     2641 cgacggtcag cttgcgatcg ttgcgcagga tccgttcgag gcggcgcgcc aaatcgagcg
     2701 cgacgtcttt ctcctcgaga ccggtcggcc cctgcgcgcc caactcgacg ccgccgtgtc
     2761 ccgggtcgag cacgatcacc gtgccccggg tgcccttgcg cccgcgcgga cgctcgacgg
     2821 gcagcggcgc ctcttcgccg ccgcggctcc cctgcaggtc gatgatcagc cggaacggat
     2881 tgcgcagctc gaaggtctcg aagctgcgga agcccggtcc ggtcgccagc accaaccgcc
     2941 ggtcgtcctc catgcggaaa cgcgacagga tcgggtcgtc gagcagcagc tcgctcggct
     3001 cggcgcggac cggggtggcg tagatcacct cgatcagccc gtcctgctgc cgcacctcgt
     3061 aggcctcgga cgaggagtgg gtcagcacca agcgcatgct cgactccgac gagtcgacct
     3121 tgatctggac cgaggcgcgc tgggccgcgg caggcgtcgc cgccgccgct ccgagcaaca
     3181 ggagcgcgag cgcgactcgt cgaagctggg gggcatgttc gatcaacgcg ctgaaccttc
     3241 cggcggagcc taagattctg tcacagcgcg cggaatgcaa caaccgggcc gagggcgccg
     3301 gcgcggccgg tcaggcgaac cgcacctggt agatcggcgg caggtgggtc gagaccgttt
     3361 gccagctctg gcccgcgtcg tccgagaccc acaagccgcc ggtcgtcgac cccatcgcca
     3421 gccgctcgcc gctgcggtcg acgtcgagcg cgtggcggta gacgagatcg taggcgtg
//
LOCUS       DASPGN010000157        13653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_494796, whole genome shotgun sequence.
ACCESSION   DASPGN010000157 DASPGN010000000
VERSION     DASPGN010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 13653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13653
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494796"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VD788_04345"
     CDS             <1..541
                     /locus_tag="VD788_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXV75526.1"
                     /translation="GATATPGADAAGLLLGAGAHRLLSLVIAVAILGSANVTLMAGAR
                     IYYAMACDRLAPRPLARANRRGVPATALWWSGAWSALLAAAAPVESLYRWATLAILLL
                     SSLTVASLFVLRRRDPRFRSFRCPGYPLTPALYLVASLTVAVSSGFYDPWGALWGVLL
                     VAAGFAAYVVWRHAAVSPR"
     gene            complement(528..2021)
                     /locus_tag="VD788_04350"
     CDS             complement(528..2021)
                     /locus_tag="VD788_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HXV75527.1"
                     /translation="MPAAVPPDASRPHPDQLRQTAGFVLAPLAFVAVWLAPVGLDPVA
                     HRLAAVFALVVVLWISEAIPLAATALVGPALCVPLGIASAKDAFAEFGNPIIFLFLGS
                     FVIAAAMRTHGLDRRIALFVLSRRWVGESPTRILFAFGAIAALLSMWMSNTATTAMML
                     PIGCGVLATLAPQAGSPRASYGTGLMLMIAYASSIGGIATPVGTPPNLIALGLIEELG
                     GRRYSFLEWMSFGVPVSVVLFAATFVLIRRLFRPPAVRLAGAASTIRRERAALGAMSA
                     GERAVLTSFLLAVSLWTLPGLVTLAFGAAHPATLRIESLLPNEGPSAILAASVLFVLP
                     VDWRARRFALSWQEAARVDWGTILLFGGGLSLGSLAFSTGLAGALGDRFLAATGDLPV
                     LLLSLCGLFLANVMTEFMSNTATANLLVPMFLALAGVTTGDALLATVAVTIGCSLAFC
                     FPVATPPNAIVYGSGLVPLTQMIRAGVWLDLGCGLLAWVVLAALIGG"
     gene            complement(2032..4110)
                     /locus_tag="VD788_04355"
     CDS             complement(2032..4110)
                     /locus_tag="VD788_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXV75528.1"
                     /translation="MPVLALLSVAACDMPEEGGELDRGSAEIDDLVRIRERGTLRVLR
                     PQQLDAERLPRAGHAFGVEELVAAYARDEGLEARWVSVDSRADLIPALLDDRGDMIAG
                     NLTVTDERKRQIAFTVPVATVRQQIVTRADDHRVKTPADLAGRTIAVRRSSPFWQTVR
                     ELRGRHPEIGVELVPEPYDAEEIIRRVAAGEYDLTVADSDVVQGVLTYRDDVKPVLDL
                     TRERPVAWAVRHDSRRLLGSLNRFLSETQLARRPQQHHTGDLDEIRNGRTLRVLTRNN
                     AATYFVYRGELIGFEYEFVREFARRQGLRLEMIVPPSGDDLLPWLLDGRGDLIAAALT
                     PTAARRAMGVQFSQPYNFVSQLVVARADEDRLTQPADLAGRSVVVRPSSAYRTTLEQL
                     RYTGIDVAIVDAPEQMETSEIIDRVASGEFDLTLADSHIVEIELTWRDDVRAVFALTE
                     EKPLGWVVRGDNPQLLGAVNDYIEEEYRGLFYNVIYDRYFNDEHRIRRHYQAWESSSG
                     ELSPYDALIRRYAERYGFDWRLIVALMYQESRFDPLAVSFAGARGLMQVLPGTALELG
                     VGEKQMDDPEASIRVGVRYLAWLRDRFDQELPVRDRMWLSLAAYNAGYGHVSDARRIA
                     AEQGLNPNRWFGNVERAMLLLDRPDYARDTRFGYCRCTEPVNYVREIVARYNAYIESA
                     RAGREERAAL"
     gene            4234..4983
                     /locus_tag="VD788_04360"
     CDS             4234..4983
                     /locus_tag="VD788_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75529.1"
                     /translation="MEPGLAANPSYLAVIGLLCGLFGFRVGWRTKLPALLPLVQGLVG
                     FVAFATAWRAGGAIASAVAVGGWAIGTTLLSLYVFARDETIDDRVIGARRYREFRFAA
                     LTSPTGRAPWSWSRLARDAGVTLLFAVAAATTVNLVSLVIGAALLNTMNAWVAVLLRS
                     MHGGWIVRLLVWDGWNLVRATSLVMLGSGLAAPLAARLGYPAPWYEVRWLLGVGGIGL
                     VLGPVLRLLLAGTTGRLLGRTVHLGPRDGGG"
     gene            4990..6003
                     /locus_tag="VD788_04365"
     CDS             4990..6003
                     /locus_tag="VD788_04365"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV75530.1"
                     /translation="MRSVFHPFLPNGPAGDPVLWVDLPDEGHSLMLDLGRLDAITPRK
                     LLRVGRVVVSHTHIDHFIGFDALLRLVLGRERELVLSGPDGFLERVAGKIHGYSWNLI
                     ERYPVVLVAEEVAGGVVRSVRYTGANRMRGEPPVERPVTGALHAHRAYTVHVDSFDHG
                     IPVLGVALRETEHLSVNRDRLQRLGLEPGEWLGTLKNAVRRGVSADREIAATTSDGGT
                     RSLRVGRLEDEILLRTRGQTIGYLTDLAHTQANLDRAARLLRDVDLLVCEAAFLDADR
                     DLACERRHLTARQAGELARACGARKLAPFHFSPRYAGRHDRLRAEAAEAFGGPVVSLP
                     TGP"
     gene            6056..7441
                     /locus_tag="VD788_04370"
     CDS             6056..7441
                     /locus_tag="VD788_04370"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXV75531.1"
                     /translation="MARGKSSRLLRRFLILVALAGLAFLGLAGFRAGPAPVVEIEAGL
                     PGIGKRTPFTIVVREPTRGLSAVRVELVQGERVELLEERSYEPRQPWEFWGPRDERDE
                     IRVEVGSETIKGLEPGEAELRVVADRAPAWLSRPAPMIDGLTLPVKLRPPTLQIVSRH
                     TYVDQGGSEAVIYKVGASSIRDGVQAGEWFFPGYPLPGGQADDRFAWFGVPFDMGDAS
                     DIKLIATDDVNNLSHAPFIDQFRPKPFRNDTITLSDSFMERVVPAILAQSPEIEDRGD
                     LLQNYLAVNGELRRLNAEALVELSRQSVLQFLWDEHFIPMRNAKVMSNFADRRSYLSD
                     GETVDRQDHLGYDLASTALAEIQAANDGVVVMARYFGIYGNTVVIDHGYGLLSLYGHL
                     SSIEVEAGQSVERGQAIGRSGATGLAGGDHLHFTMLLHGLPVNPSEWWDAHWIHDRLK
                     LKLGDALPFDD"
     gene            complement(7443..9338)
                     /locus_tag="VD788_04375"
     CDS             complement(7443..9338)
                     /locus_tag="VD788_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75532.1"
                     /translation="MVRFRSWIVLAVLIAAGGVVAAGDVSLTYRFTNQGLPLDDPSKA
                     RFYVYADDNDEVYLAWGSGERPTVTVPEGSYHIVVKYVDDLIEFERVFEEVELADDVE
                     IDFDFDIPVAHLTMWITSGGFPIPRHAGRYSLYRRGEHDKPLVSRRPGESVTIAPGSY
                     DVEIVYRGPDGLESQWLEDYALDGMRQETIDLGTSNVQLRMTLVDGGRALEPGEGEYR
                     VYAAGSRDLPLAEAPSGEAVMLSPGTYDVGLFYTSGGKRAERWVVGVEATGHVDREVD
                     VSTRSDRLTVDIRHGGRTLPDAWFTVYPAGDRTSKAYEGNSGQPLEIEPGTYDIGCFV
                     RRGGLRASQWIENKQLEGPVELTVELDYREASLRVEPASRRPRETGAANLLIVVDSSA
                     SMMEPLDGSTKMEQLRANLPRGIDTLLGTDINVGLRVYGITPRARQNCTDSTLLIPVR
                     GLDRRAIERAVDLLRPTGSAPIAHSLEQAASDLPQGGPQSMLLITGGGETCGGDACTA
                     ASSLLRDGGVSRIYVIALGADRETRRRLDCVGEVRTARSQLELQAAIREVLREVRRGD
                     EDAVLVFDPSGDNLIASGGLRERIQLTAGTYDVVIRNGDKTYRWDGLDLHGDVEAKAG
                     SRPPRGR"
     gene            complement(9500..10411)
                     /locus_tag="VD788_04380"
     CDS             complement(9500..10411)
                     /locus_tag="VD788_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXV75533.1"
                     /translation="MSAPGRATIVVVFAVLCAIWGSTWAVIRIGLRGVPPFSGVALRF
                     AIASVVLGLLALAFRLPLGRTRRERALWWVNGVLAFSVSYGVVYWSEQWVPSGLTAVL
                     FATYPLFVAIISHFILPEEALSRAELIGVIGGFAGVGLIFSEDLAALGGPQVAIASAV
                     MLLSPSASALSSVAIKRWGAGVHPLSLTAVPMAICACLTGAVALSTERELTFSWNRTS
                     IGALLYLALAGSAVTFTLYYWLLSHLPAKRLALIAYIIPVEAVLIGVFLGERLTPRIL
                     AGSILVVFGVALAVHRRGRRSPRTAGG"
     gene            complement(10408..11304)
                     /gene="rfbA"
                     /locus_tag="VD788_04385"
     CDS             complement(10408..11304)
                     /gene="rfbA"
                     /locus_tag="VD788_04385"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HXV75534.1"
                     /translation="MKGIILAGGSGTRLHPLTIAISKQLMPIYDKPMIYYPLSTLMLA
                     GIREILVITTPEDRPSYERLLGDGEQVGLRLSYAEQPAPDGIAQAFVIGGDFIGSDRV
                     ALALGDNVFYGHGLPETLRRAAGRSRGATVFGYRVRDPERYGVVEFDATGKVIRLEEK
                     PAKPRSPYAVTGLYFYDRRVVEIAAKLEPSARGELEITDVNRAYLATGELHVEMLGRG
                     TAWLDTGTHQSMLQAANYIQAIEERQGLMVACIEEVAFKMGFIDADRVRRAAERMRKS
                     GYGRYLLAMLDDEGNAGDRPDE"
     gene            complement(11301..12395)
                     /gene="rfbB"
                     /locus_tag="VD788_04390"
     CDS             complement(11301..12395)
                     /gene="rfbB"
                     /locus_tag="VD788_04390"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HXV75535.1"
                     /translation="MAGLLVTGGAGFIGSNFVRKALAETDHTLIVLDKLTYAGSLHNL
                     DGVADEPRLRFVRGDIADRELTGELLGRGGVTAIVNFAAESHVDRSIDSPRPFVDTNI
                     VGTFELLVAARDHLARLDEPARKAFRFLHVSTDEVFGSLGADGRFSETTRYAPNSPYA
                     ASKASADHLVRAFHHTYGLPTLLTNCSNNYGPYQFPEKLIPLVILNAADGEELPIYGD
                     GMNVRDWLYVEDHCDGVLRVLDAGRPGESYNIGGDCERPNVAVVDAILDALERRLPAA
                     TNERLAARGVGAYRELKRFVPDRPGHDRRYAIDASRIRAELGWSPEHDFDSGIDATVS
                     WYLGHRDWCDAVQREAGYGRERLGLKGDDA"
     gene            complement(12529..13635)
                     /locus_tag="VD788_04395"
     CDS             complement(12529..13635)
                     /locus_tag="VD788_04395"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXV75536.1"
                     /translation="MSRIRSIAVWLQVAAALVSASDATGGSRAQAVSESFTIDVARLE
                     IRNLIGEIDVTTHDGASFEVAVSYQGKDAVRHPPAVVRESGRDGEQLTIRFPESRRYV
                     YPRLGPGSATQISENVDGGWFSKMMSGLFGESIKVAGSGSGLEVWADVEVQVPSGASL
                     VVRHGVGAARALDVAADLELELRSGRVEAARIGGNLTIETGSGQIDVDGVDGAVELST
                     GSGSVDAREIRGPAASIATGSGGVTLVRIDSRSLSVATGSGRVDADGVSADRATIATG
                     SGGVRLEMDRMGDGSFEIGTGSGRVVLEVPPSASLDVHAETGSGGIDLEMGGAPLNMT
                     HRDRDEVRFSVGGGEAKVRISTGSGGVVIAQSTR"
BASE COUNT         2080 a         4896 c         4629 g         2048 t
ORIGIN      
        1 cggtgcgacc gccacgccgg gtgccgacgc ggccggcctg ctgctcgggg ccggagcgca
       61 tcgtctgctc tcgctcgtca tcgccgtcgc catcctcggc tcggccaacg tcaccctgat
      121 ggccggcgcg cgcatctact acgcgatggc gtgcgaccga ctcgccccac ggccattggc
      181 gagagccaat cgccgcggcg tccccgcgac cgccctgtgg tggagcggcg cgtggtccgc
      241 gctgctcgcc gccgccgctc cggtcgaatc gctctaccgc tgggccactc tcgcgatcct
      301 gctgctgtcc tcgctgacgg tcgcgtcgct gttcgttctc cgccggcgcg acccgcggtt
      361 ccggtcgttc cgctgcccgg gctacccgct gacgcccgca ctctacctcg tcgcgtcgct
      421 gacggtcgcg gtgtcgagcg ggttctacga cccgtggggc gcgctgtggg gcgtgctgct
      481 cgtcgccgcg ggcttcgcgg cctacgtcgt ctggcgccac gccgcggtca gcccccgatg
      541 agcgccgcca gcacgaccca cgccaacaac ccgcaaccga gatcgagcca gaccccggcg
      601 cggatcatct gcgtcaacgg cacgagaccg ctgccgtaga cgatcgcgtt cggtggggtc
      661 gcgaccggga agcaaaacgc caggctgcaa ccgatcgtca ccgcgaccgt cgccagcagc
      721 gcgtcgcccg tcgtgacgcc ggccagcgcg aggaacatcg gcaccagcag attcgccgtg
      781 gcggtgttcg acatgaactc ggtcatcacg ttggccagga acaggccgca caacgacaag
      841 agcagcacgg gcaaatcgcc ggtcgcggcg aggaaccgat cgccgagcgc cccggccaga
      901 cccgtcgaga acgccagcga gccgagcgac agaccgccgc cgaacagcag gatcgttccc
      961 cagtcgactc gcgccgcctc ctgccagctc aacgcgaaac ggcgcgcgcg ccagtcgacc
     1021 ggcaggacga acagcaccga cgcggccagg atcgccgacg gtccctcgtt cggcagcagc
     1081 gactcgattc gcagcgtcgc cgggtgagcc gcgccgaacg ccagcgtgac gaggcccggt
     1141 aacgtccaca acgacactgc cagcaagaac gacgtgagca ccgcgcgctc gcccgcgctc
     1201 atcgcgccga gcgcggcacg ctcccgccga atcgtcgacg ccgcaccggc gagtcgcacc
     1261 gcgggcggac ggaacagccg gcgaatcagc acgaacgtcg ccgcgaacag cacgaccgac
     1321 accggaacgc cgaaactcat ccactccagg aacgagtacc gacgcccgcc gagctcctcg
     1381 atcaggccga gcgcgatcag gttgggcggc gtacccaccg gcgtcgcgat gccaccgatg
     1441 ctcgacgcgt aggcgatcat cagcatgagg ccggtcccgt agctggcgcg cgggctgccg
     1501 gcctgcggcg cgagggtcgc cagcacaccg cagccgatcg gcagcatcat cgccgtcgtc
     1561 gccgtgttgg acatccacat cgacagcaac gccgcgatcg cgccgaaggc gaacaggatg
     1621 cgcgtcggtg attcgccgac ccagcggcgc gacaacacga acagcgcgat ccgccggtcg
     1681 agaccgtgcg ttcgcatcgc ggcggcgatg acgaacgaac cgagaaacaa gaagatgatc
     1741 gggttgccga actcggcgaa cgcgtccttg gccgaagcga tgccgagcgg cacgcacaac
     1801 gccggtccga cgagcgccgt cgccgccagc gggatcgcct cgctgatcca cagcacgacg
     1861 accagtgcga agacggcggc cagccgatgc gccaccgggt cgaggccgac cggcgccagc
     1921 cacaccgcga cgaaggccag cggcgccaac acgaaaccgg cggtctgtcg gagctggtcg
     1981 gggtgtgggc gcgaagcgtc gggggggact gctgccggca tcgcgccgat tctacaacgc
     2041 cgcgcgctcc tcgcgacccg cccgcgcaga ctcgatgtac gcgttgtagc gcgcgacgat
     2101 ctcgcgcacg tagttgaccg gttcggtaca gcgacaatag ccgaagcgcg tgtcgcgcgc
     2161 gtagtccggc cggtccaaca gcaacatcgc ccgctcgacg ttgccgaacc agcgattggg
     2221 gttcaggccc tgttcggcgg cgatacgccg cgcgtcgctg acgtgaccgt agccggcatt
     2281 gtaggccgcc aggctgagcc acatccggtc gcgcaccggc agctcctggt cgaaccggtc
     2341 gcgcaaccac gccaggtagc gcacgccgac ccggatgctc gcttcagggt cgtccatctg
     2401 cttctcgccg acccccagct cgagcgcggt gcccggcagc acctgcatca gcccgcgagc
     2461 gccggcgaac gacaccgcca gcggatcgaa ccggctttcc tggtacatca acgccacgat
     2521 caaccgccaa tcgaagccgt accgctcggc gtagcgccgg atcagcgcgt cgtacggtga
     2581 cagctcgccg gacgacgatt cccacgcctg atagtggcgg cggatgcgat gctcgtcgtt
     2641 gaagtagcga tcgtagatca cgttgtagaa caggccgcgg tactcctcct cgatatagtc
     2701 gtttaccgcg ccgagcagct gcgggttgtc gccgcgcacg acccaaccga gcggcttctc
     2761 ctcggtcaac gcaaagaccg cgcgcacgtc gtcgcgccag gtcagctcga tctcgacgat
     2821 gtgactgtcg gccaacgtca ggtcgaattc gccgctcgcc acgcgatcga tgatctcgga
     2881 tgtttccatc tgctcgggcg cgtcgacgat ggcgacgtcg atgccggtgt aacgcaactg
     2941 ctcgagcgtg gtgcggtagg cgctgctcgg ccgcacgacg accgatcgcc cggcgaggtc
     3001 ggcgggctgc gtcaagcgat cctcgtccgc gcgcgcgacg accaactgcg agacgaagtt
     3061 gtacggctgc gagaactgga ctcccatcgc gcgccgggcc gcggtcggcg tcagcgccgc
     3121 cgcgatcaga tccccgcgcc cgtcgagcag ccacggcagc aggtcgtccc cgctcggcgg
     3181 cacgatcatc tcgagccgca acccctgtcg gcgggcgaac tcgcgcacga actcgtactc
     3241 gaagccgatc agctcgccgc gatagacgaa ataggtcgcc gcgttgttgc gcgtcaggac
     3301 gcgcagcgtc cgcccgttgc ggatctcgtc gaggtcgccg gtgtgatgct gctgcggccg
     3361 acgggccaac tgggtttcgc tgagaaagcg gttgaggctg ccgagcaacc gccgggagtc
     3421 gtggcggacg gcccaggcca ccggtcgttc tcgggtcagg tcgagcaccg gcttgacgtc
     3481 gtcgcggtag gtcaacaccc cctgcaccac gtcgctgtcg gcgacggtca ggtcgtactc
     3541 gccggccgcc acgcggcgga tgatctcctc cgcgtcatac ggctcgggca cgagctcgac
     3601 accgatctcc ggatggcgtc cacgcagctc gcgcaccgtc tgccagaacg gggacgagcg
     3661 gcgcaccgcg atcgttcgcc cggccaggtc ggccggagtc ttgacgcggt ggtcgtcggc
     3721 acgcgtcacg atctgctggc gcacggtggc caccggcacg gtgaaggcga tctgccgctt
     3781 gcgttcgtcg gtcacggtca ggttgcccgc gatcatgtcg ccgcggtcgt cgagcagcgc
     3841 cgggatcagg tcggctcgcg agtcgacgga gacccagcgg gcctcgagac cctcgtcacg
     3901 cgcgtaggcc gcgaccagct cttcgacgcc gaacgcgtgg cccgcgcgcg gcagccgctc
     3961 ggcgtcgagc tgctgcgggc gcagcacgcg gagcgtcccg cgctcgcgaa tgcgaacgag
     4021 atcgtcgatc tcggccgaac cccgatcgag ctccccgccc tcttccggca tgtcgcacgc
     4081 agcgaccgac agcagcgcca gaacaggcaa gagcgctcgt acccccgacc gcccgctcat
     4141 gatccgaaaa tctttcccca atcccgcggg gcgcgcaacc ggaaaagccg gcacgcgcga
     4201 aacggcggga aaccgtggga caatggtcgg gctttggaac cgggtctcgc cgccaatccg
     4261 tcctatctcg ccgtcatcgg tctgctttgc ggtctgttcg ggttccgcgt cggctggcgc
     4321 acgaagctgc cggcgctgct gccgctggtt cagggcttgg tcggtttcgt cgccttcgcc
     4381 acggcatggc gcgcgggcgg cgcgatcgcc tcggcggtcg ccgtcggcgg ctgggccatc
     4441 ggcaccacgc tcctctcgct ctacgtcttc gcgcgcgacg agacgatcga cgaccgggtg
     4501 atcggcgcgc gtcgatatcg cgagttccgc ttcgccgcgc tgacctcacc caccggtcgt
     4561 gcgccgtggt cgtggtcgcg tctggcgcgg gacgccggcg tgacgttgct gttcgccgtc
     4621 gccgccgcga cgacggtcaa cctggtctcg ctcgtcatcg gcgcggcgct gctcaatacg
     4681 atgaacgcct gggtcgccgt cttgctgcgc tcgatgcacg gcggctggat cgtgcggctg
     4741 ctggtctggg acggctggaa cctggttcgc gcgacctcgc tggtcatgct cggcagcggc
     4801 ctggcggccc cactggccgc gcgattgggc tatcccgcgc cgtggtacga ggtgcgctgg
     4861 ttgctcggtg tcggtggcat cggactcgtg ctcgggccgg ttctgcgcct gctgctcgcc
     4921 ggcacgaccg gccggctgct cggccgtacc gtgcacctcg ggccgcgcga cggcgggggc
     4981 tgagcgccga tgcgctccgt cttccacccg ttcttgccga acgggccggc gggcgacccg
     5041 gtgctgtggg tcgacctgcc cgacgagggg cactcgctga tgctcgatct gggccggctc
     5101 gacgcgatca cgccgcgcaa gctgctgcgt gtcggccgcg tcgtcgtctc ccacacgcac
     5161 atcgaccact tcatcggttt cgatgcgttg ctgcggctgg tgctcggccg cgagcgcgag
     5221 ctggtgctga gcggccccga cgggtttctc gaacgcgtcg ccggcaagat ccacggctac
     5281 agctggaacc tgatcgagcg ctacccggtc gtgctcgtcg ccgaggaggt cgcgggcggc
     5341 gtcgtgcgct cggtgcgcta caccggcgcc aaccgcatgc gcggtgaacc gccggtcgag
     5401 cggcctgtga caggcgcgct gcacgcccac cgcgcctaca ccgttcacgt cgacagcttc
     5461 gaccacggca tcccggtgct cggcgtggcg ctgcgcgaga ccgagcacct gtcggtgaat
     5521 cgcgaccggc tgcaacgcct cgggctcgag ccgggcgagt ggctcggcac gctgaagaac
     5581 gccgtccgcc gcggcgtttc ggccgatcgc gagatcgcgg cgacgacgag cgacggcggc
     5641 acgcgttcgc tgcgcgtcgg acggctcgag gacgagatcc tgttgcggac gcgcggccag
     5701 acgatcggtt acctgaccga cttggcccac acccaggcca acctcgaccg cgcggcacgc
     5761 ttgctgcgtg acgtcgatct cctcgtctgc gaagcggcgt ttctcgatgc cgaccgcgac
     5821 ctggcctgcg agcggcgcca cctgaccgcg cgacaggcgg gcgagctcgc gcgcgcctgc
     5881 ggcgcccgca agctggcgcc gttccacttc tcgccgcggt acgccgggcg gcacgaccgc
     5941 ttgcgcgccg aggccgccga agccttcggt ggtccggtcg tctcgctccc gaccgggccg
     6001 tagatcacgc gatgctatag ttccgtctcg tcctcggcgg ctagtggagt ggaacgtggc
     6061 gcgtggcaaa tcgagccggt tgctcagacg atttctgatt ctggttgcgc tcgcgggcct
     6121 ggcgtttctc ggtttggcgg gcttccgcgc cggaccggcg ccggtcgtcg agatcgaggc
     6181 cggtctgccc ggtatcggca aacgaacgcc gttcacgatc gtcgtccgag aaccgacccg
     6241 cgggctgtcg gcggtgcgtg tcgagctggt ccagggcgag cgggtcgagc tgctcgaaga
     6301 gcgcagctac gagccgcgcc agccatggga attctggggt ccgcgcgatg agcgagacga
     6361 gatccgggtc gaggtgggca gcgagacgat caagggcctc gagccaggcg aggccgagct
     6421 gcgcgtcgtc gccgaccgcg cgccagcctg gctcagccgc cccgctccga tgatcgatgg
     6481 cttgacgctc ccggtcaagc tgcgtccgcc cacgctgcag atcgtctcgc gccacaccta
     6541 cgtcgaccag ggcggctccg aagcggtgat ctacaaagtg ggcgcctcgt cgatacgcga
     6601 cggcgtgcag gccggcgagt ggttctttcc cggctacccg ctgcccggcg gccaggcgga
     6661 cgaccgcttc gcctggttcg gcgtgccgtt cgacatgggc gacgcgtccg acatcaagct
     6721 gatcgcgacc gacgacgtca acaacctgtc tcacgcaccg ttcatcgacc aattccggcc
     6781 gaagccgttt cgcaacgaca cgatcacgct gtccgactcg ttcatggagc gcgtcgtgcc
     6841 ggcgatcttg gcccaatcgc ccgagatcga ggatcgcggc gacctgctgc agaactacct
     6901 cgccgtcaac ggcgagctgc ggcggctgaa cgccgaagcg ctggtcgagc tgtcacgtca
     6961 atcggtgctg cagttcctct gggacgagca tttcatcccg atgcgaaacg ccaaggtgat
     7021 gtcgaacttc gccgaccgcc gcagctacct ctccgacggc gagacggtcg atcgacagga
     7081 ccatctgggc tacgacctcg cgtcgaccgc gttggccgag atccaggcgg ccaacgacgg
     7141 cgtcgtggtg atggcgcgct acttcggcat ctacggcaac acggtggtga tcgaccacgg
     7201 ctacggactg ctcagcctgt acggccacct gtcgagcatc gaggtcgagg cggggcagtc
     7261 ggtcgagcgc ggccaggcga tcggccgctc gggcgcgacg ggtctcgccg gcggggacca
     7321 cttgcacttc accatgctgc tgcacggcct gccggtcaat ccgtcggagt ggtgggacgc
     7381 gcactggatc cacgaccggc tgaagctgaa actcggcgac gcgctgccgt tcgacgactg
     7441 agctagcgtc cacgcggcgg gcgcgagcct gccttggcct cgacgtcgcc gtgcaggtcg
     7501 agcccgtccc aacggtaggt cttgtcgccg ttgcggatca cgacgtcgta ggtgccggcg
     7561 gtgagctgga tccgctcgcg cagtccgccg ctcgcgatca agttgtcgcc gctggggtcg
     7621 aagaccagca cggcgtcctc gtcgccgcgg cgtacctcgc gcagcacctc gcggatcgcg
     7681 gcctgcagct cgagctgcga gcgcgccgtc cgcacttcgc cgacgcagtc gagccgccgc
     7741 cgcgtctcac ggtcggcgcc gagcgcgatg acgtagatcc gcgagacccc gccgtcacgc
     7801 agcaggctgc tggcggcggt gcaggcgtcg ccgccgcagg tctcgccgcc gccggtgatc
     7861 agcagcatcg actgggggcc gccctgtggc aggtcggatg ccgcctgttc gagcgagtgc
     7921 gcgatcggag cggagccggt cgggcggagc aggtcgaccg cgcgctcgat cgcgcgccgg
     7981 tcgagaccgc gcaccgggat gagcaaggtc gagtcggtgc agttctgtcg ggcgcgcggc
     8041 gtgatgccgt agacgcgcaa cccgacgttg atgtcggtac cgagcagggt gtcgatgccg
     8101 cgtggcaggt tggcccgcag ctgctccatc ttggtgctgc cgtccagcgg ctccatcatc
     8161 gaggcggagc tgtcgacgac gatcagcaga ttggccgcgc cggtctccct cgggcgacgc
     8221 gacgccggtt cgacgcgcag cgacgcctcg cggtagtcca gctcgaccgt cagctcgacc
     8281 ggcccctcga gctgcttgtt ctcgatccac tggctggcgc gcagaccgcc gcgcctgacg
     8341 aagcagccga tgtcgtacgt tcccggttcg atctcgagcg gctgcccgct gttgccctcg
     8401 tacgccttgc tcgtgcggtc gcccgccggg tagacggtga accacgcgtc cggaagcgtc
     8461 cggccgccgt ggcggatgtc gaccgtcagc cgatccgacc gcgtggaaac gtcgacttcg
     8521 cgatcgacgt gaccggtggc ctcgacaccg accacccagc gttccgcccg cttgccgccg
     8581 ctcgtgtaga acaacccgac gtcgtaggtc cccggcgaca acatcacggc ttcgcccgac
     8641 ggggcctcgg ccagcgggag atcgcgcgag cccgccgcat agacgcggta ctctccctcg
     8701 ccgggttcga gcgcgcgccc gccgtcgacc agcgtcatcc gcagctggac gttcgacgtc
     8761 ccgaggtcga tcgtctcctg gcgcattccg tcgagcgcgt aatcctcgag ccactgactc
     8821 tccagcccgt ccggcccgcg gtagacgatc tcgacgtcgt agctgccggg ggcgatcgtc
     8881 accgactcgc ccgggcgccg cgagacgagc ggcttgtcgt gttcaccacg gcgatagagg
     8941 ctgtagcgcc cggcgtgacg cgggatcgga aagcctccgc tggtgatcca catcgtcaag
     9001 tgagcaaccg gaatgtcgaa gtcgaagtcg atctcgacgt catcggccag ctcgacctcc
     9061 tcgaacaccc gttcgaactc gatcaggtcg tcgacgtact tgacgacgat gtggtagctg
     9121 ccctcgggca cggtcaccgt cggccgctcg ccgctgcccc aggcgaggta gacttcgtcg
     9181 ttgtcgtcgg catagacgta gaaccgcgcc ttcgacgggt cgtcgagcgg cagtccctgg
     9241 ttggtgaagc gataggtcag cgacacgtcg ccggcggcca cgacgccgcc ggccgcgatc
     9301 agcacggcca gcacgatcca actgcgaaat ctcaccatcg gtcctctcgg cagcattcta
     9361 cccggccgtt tcgacgaccg gccacgattg cagtaactct ctggtcgatc gtcggcctga
     9421 gggcatccgc ggcgtacctc taccaatctc gatcgtttgg cggcagaagg ccagcccgcg
     9481 gtggcgacgc cccgaccggt cagccgccgg ccgttcgcgg cgacctccgc ccgcggcggt
     9541 gaacggcgag cgccacgccg aagacgacga ggatcgaacc ggcgaggatg cgcggcgtca
     9601 gccgttcgcc gaggaacacc ccgatcagga ccgcctcgac cgggatgatg taggcgatca
     9661 gcgccaaccg cttggccggc aggtgcgaca gcagccagta gtacagcgtg aacgtgaccg
     9721 ccgagccggc gagcgccagg tacagcagcg ctccgatcga ggtgcggttc cagctgaagg
     9781 tcagctcgcg ctcggtcgac aacgcaacag ccccggtcag gcaggcgcag atcgccatcg
     9841 gcaccgcggt cagcgacagc gggtggacgc cggcgcccca gcgcttgatc gccacgctcg
     9901 acagcgccga ggccgacggc gacagcaaca tcaccgccga ggcgatcgcg acctgcgggc
     9961 cgcccagcgc cgcgaggtct tcggagaaga tcaggccgac cccggcgaag ccgccgatca
    10021 cgccgatcag ctcggcgcgg ctgagcgctt cttccgggag aatgaagtgc gagatgatcg
    10081 ccacgaacaa cgggtaggtc gcgaacaaca ccgcggtcag acccgacggc acccactgct
    10141 cgctccaata gacgacgccg taggacacgg agaacgccaa cacgccgttg acccaccaca
    10201 gcgcgcgttc gcgtcgcgtc cgcccgagcg gcaggcgaaa cgccagcgcc agcagaccga
    10261 gcacgaccga cgcgatggcg aagcgcaacg cgacgccgct gaacggtggc acgccgcgca
    10321 aaccgatccg gatcaccgcc caggtgctgc cccagatcgc gcacagcacg gcgaagacga
    10381 cgacgatcgt cgcgcgcccg ggcgcgctca ttcgtcgggc cgatcgcccg cgttcccctc
    10441 gtcgtcgagc atcgccagca ggtaccggcc gtaccccgac ttgcgcatgc gctcggcggc
    10501 ccggcgcacg cggtcggcgt cgatgaagcc catcttgaac gcgacttctt cgatacaggc
    10561 caccatcaac ccctggcgct cttcgatcgc ctggatgtag ttggccgcct gcagcatcga
    10621 ctgatgggtg ccggtgtcga gccatgcggt cccgcggccg agcatctcga cgtgcagttc
    10681 acccgtcgcg agataggccc gattgacgtc ggtgatctcg agctcgccgc gcgcggacgg
    10741 ctcgagcttc gccgcgatct cgacgacgcg ccgatcgtag aagtacaggc cggtgacggc
    10801 gtagggcgag cgcggtttgg ccggcttctc ctcgagacgg atcaccttgc cggtcgcatc
    10861 gaactcgacc accccgtagc gctctggatc acgcacgcgg tagccgaaca ccgtcgcacc
    10921 gcggctgcgg ccggcggcgc gcctcagcgt ttccggcagc ccgtgcccgt agaacacgtt
    10981 gtcgccgagc gccagcgcca cccgatcgga gccgatgaag tcgccgccga tgacgaacgc
    11041 ctgcgcgatg ccgtcgggcg caggctgttc ggcgtagctc agccgcaggc cgacctgctc
    11101 gccgtcgccc aacagccgct cgtaagacgg gcggtcttcg ggcgtggtga tcacgaggat
    11161 ctcgcggatg ccggccagca tcagcgtcga cagcgggtag tagatcatcg gcttgtcgta
    11221 gatcggcatc agctgcttgc tgatcgcgat cgtcaacggg tgcagccgcg tcccgctgcc
    11281 gccggcgagg atgattccct tcacgcgtcg tctcccttca gccccagccg ctcgcgcccg
    11341 tagcccgcct cgcgctggac cgcgtcacac cagtcgcggt ggccgagata ccacgacacc
    11401 gtcgcgtcga tgccgctgtc gaagtcgtgc tcgggcgacc agcccagctc ggcgcgtatt
    11461 ctcgacgcgt cgatcgcgta gcgccggtcg tggcccggcc gatcgggcac gaaccgcttc
    11521 agctcgcggt aggcgcccac gccgcgggcc gccaaccgtt cgttggtcgc ggccggcaga
    11581 cgtcgttcga gcgcgtccaa gatcgcgtcg acgaccgcga cgttcggtct ctcgcagtca
    11641 ccaccgatgt tgtagctctc tcccgggcgg ccggcatcga gcacgcgtag cacgccgtcg
    11701 cagtggtcct cgacgtacag ccagtcccgc acgttcatcc cgtcaccgta gatcggcagc
    11761 tcttcgccgt cggcggcgtt gaggatcacg agcgggatca gcttctcggg aaactggtac
    11821 ggaccgtagt tgttcgagca gttggtcagc agcgtcggca ggccgtaggt gtggtggaac
    11881 gcgcgcacca ggtgatcggc cgaggccttc gacgcggcgt agggcgaatt gggtgcgtag
    11941 cgcgtggttt cgctgaaccg gccgtccgcg ccgagactgc cgaacacctc gtccgtcgat
    12001 acgtggagga agcgaaaggc cttgcgcgcc ggctcgtcga gccgcgccag gtggtcgcgg
    12061 gcggcgacca agagctcgaa cgtcccgacg atgttggtgt cgacgaacgg gcgcggcgag
    12121 tcgatgctgc ggtcgacgtg cgattcggcg gcgaagttga cgatcgccgt caccccgccg
    12181 cggcccagca gctcgccggt cagctcgcgg tcggcgatgt cgccgcgcac gaagcgcagg
    12241 cgcggctcgt cggccacgcc gtcgaggttg tgcaggctgc ccgcgtaggt cagcttgtcg
    12301 agcacgatga gcgtgtggtc ggtctcggcc agcgccttcc tgacgaagtt cgagccgatg
    12361 aacccggcac cgccggtgac gagcaaccca gccatgcgcg ccatcctacc cgctcggcgg
    12421 gacggaatca cggccgggtt gtgacagggg caaaggggcc ggcgggcgac gtcgggtcgg
    12481 gggagggcgg cagccggccc tcgtgcgccc gccggcaagt ctggaacgct accgagtcga
    12541 ctgcgcgatg acgacgccgc cgctgccggt gctgatccgc accttggcct cgccgccgcc
    12601 gacgctgaac cggacctcgt cgcgatcccg gtgggtcatg ttcagcggcg cgccgcccat
    12661 ctcgagatcg atgccgccgc tgccggtttc ggcgtggacg tcgagcgaag cgctcggagg
    12721 gacctcgagc accacccggc cgctgccggt gccgatctcg aacgatccgt ctcccatccg
    12781 gtccatctcc aaccggacgc cgccgctacc ggtggcgatc gtcgcgcggt cggcgctgac
    12841 gccgtcggcg tcgacccggc cgctgccggt cgcgaccgac agcgagcggc tgtcgatccg
    12901 cacgagcgtc acgccgccgc tgccggtggc gatgctcgcc gccggcccgc ggatctcccg
    12961 ggcgtcgacc gagccgctgc cggtcgacag ctcgaccgcg ccgtcgacgc cgtcgacgtc
    13021 gatctgcccg ctgccggttt cgatcgtgag attgccgccg atgcgcgcgg cctcgacccg
    13081 accggagcgc agctcgagct cgagatcggc cgcgacgtcg agggcccgtg ccgctccgac
    13141 cccgtggcgt acgacgagcg acgcacccga cggcacttgc acctcgacgt cggcccacac
    13201 ttcgagtccc gacccgctgc cggcgacctt gatcgactcg ccgaacagcc cactcatcat
    13261 cttggagaac cagccgccgt cgacgttctc gctgatctgc gtggccgagc ccgggccgag
    13321 ccgcgggtag acgtagcgcc gcgactcggg gaagcggatc gtcagctgct cgccgtctcg
    13381 gcccgactcg cgcacgacgg cgggtgggtg gcggacggca tccttgccct gatagctcac
    13441 ggcgacctcg aacgaggccc cgtcgtgggt ggtcacgtcg atctcgccga tcaggttgcg
    13501 gatctcgaga cgcgccacgt cgatggtgaa gctctcgctg accgcctgcg cccgcgaccc
    13561 gcccgtcgcg tcggacgccg agaccagcgc cgccgccacc tgcaaccaga cggcgatcga
    13621 tcggattcga ctcatggctc ctcccacgcc gcg
//
LOCUS       DASPGN010000158         4422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1548533, whole genome shotgun sequence.
ACCESSION   DASPGN010000158 DASPGN010000000
VERSION     DASPGN010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1548533"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..695)
                     /locus_tag="VD788_04400"
     CDS             complement(102..695)
                     /locus_tag="VD788_04400"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXV75537.1"
                     /translation="MTTDLTIHGFETSNNIKVRVALGYKRIPYAFRAIDPADRSEILR
                     LSGQRLTPVMQHGDVVLFDSAAILRYLEANFRDRPPLFGASRDEQWAIEDLELFARHT
                     LAGPMMDVVHRRVASGSVDAELQSRCQARFAAEVGRLVAALGEREWLVGDRMSAADVT
                     AAPVIRRVRSAGLFELPPAAERLAPWVERVMAYDGQR"
     gene            779..1564
                     /locus_tag="VD788_04405"
     CDS             779..1564
                     /locus_tag="VD788_04405"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXV75538.1"
                     /translation="MNRYTVLLLMGCVACATPETPIHEREIVDLTWTLDETTIAWPTS
                     PGFTLETQFDGITDGGWYYLSHIYHGPEHGGTHIDAPIHFHEGRDTTDEIPLTRLIGP
                     GVKIDLRHQAADDRDYRVGLADFERWERAHGELPEGAIVLLDTGFADYWPDRRRYMGT
                     DGRGQEAVADLRFPGLDPATVDWLVGTKKIRAIGIDTASIDYGQSTDFAAHVALMEHN
                     VPVFENLARLDRLPPRDFTVVALPAKIGGGSGGPLRIIALLEG"
     gene            complement(1577..2950)
                     /locus_tag="VD788_04410"
     CDS             complement(1577..2950)
                     /locus_tag="VD788_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXV75539.1"
                     /translation="MKIDVLRGSLRGRVVEPDDPGYDDDRALYNGMIDKRPRAIAHCT
                     DVADVIAAVRFAAEHEMLLAIRGGGHNGPGLGSCDGGLVLDLSAMNDRRVDIANATVR
                     VGPGCTQGDVDHATHPFGLAVPAGIVSTTGIAGLTLGGGHGYLTRRFGLTIDNLLEAD
                     VVLADGSFVTCNQRQHDDLFWAVRGGGGNFGVVTSFKFRAQPVRTIYGGPVFFDAEHT
                     REIMEWYRDWLPTAPREMYLFCGLKTVPDADPFPAEIRLSRICALIGCWSGPTGEGQA
                     AFDRALAELPAPIFRHTGAMPFPMLQGLFDPLLPKGLQWYWKGDFVRELTDTAIDVHA
                     EHGRATPTPLSLMHLYPIDGAVHDVAASATAWSYRDVRWSMVIAGIDPDPANAGKLTS
                     WAKGYWDALRGQTAGGAYVNFMMEEGQDRVQATYGTNYARLVRIKTKYDPSNVFRVNQ
                     NIRPAAA"
     gene            complement(3058..3510)
                     /locus_tag="VD788_04415"
     CDS             complement(3058..3510)
                     /locus_tag="VD788_04415"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXV75540.1"
                     /translation="MDSFVKRSRIDAPAAAVFRWHMQADAFERLMPPWERVEVIDRTG
                     GIDRIGSRVTLLVGRWPLRMTWVAEHTHFEADRMFRDVQLRGPFARWEHTHLVQPDGP
                     AACWLEDRVDYALRFGRLGRWLWGSIVERKLQRIFDYRHRITTHHLTR"
     gene            complement(3520..3996)
                     /locus_tag="VD788_04420"
     CDS             complement(3520..3996)
                     /locus_tag="VD788_04420"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HXV75541.1"
                     /translation="MRFRGLRFLPVLLVLAVLPAVAVGGGEETFGAGVSGRDTIPISV
                     LLADAGDYVGKTVRVEGVITGVCQKAGCWMTLAEDGRKDEIRVKVGDGVIVFPADGKG
                     RRAIAEGVLTKVEMTLEQTVGYLQHLAEEQGGEFDAASVTEPMTLFQIEGTGALIR"
     gene            complement(4037..>4422)
                     /locus_tag="VD788_04425"
     CDS             complement(4037..>4422)
                     /locus_tag="VD788_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75542.1"
                     /translation="FVDSLGAIAAASSAEVLVLLTPFRQDPGQPAYTPPEFYRQFRET
                     VDRASDRHGLLLLDASSLLDRSHFGPYADGPEAGRIDVLHFDAAGHRAIAAAIEGTLA
                     LPRRPAGNRTEALLHRPARPTAGSL"
BASE COUNT          716 a         1577 c         1465 g          664 t
ORIGIN      
        1 accgacgtca tcagcgccgg gacgctccag cagagataga gcgcaatcca gccgcgccat
       61 ccgcggcgga tcaaccggtc cggcgcgtcg gccatccccg gctagcgttg gccgtcgtag
      121 gccatcaccc gttcgaccca cggtgccaac ctctcggccg ccggtggcaa ctcgaacagg
      181 cccgccgacc gcacgcgacg gatcacgggt gccgccgtga cgtcggccgc cgacatgcgg
      241 tcgccgacca accattcgcg ctcgccgagc gcggcgacca gacggcccac ctccgcggcg
      301 aatcgcgcct ggcagcgtga ctgcagctcg gcgtcgaccg accccgaggc cacgcgccgg
      361 tgcacgacgt ccatcatcgg accggccaac gtgtggcgcg cgaacagctc gaggtcttcg
      421 atcgcccatt gctcgtcgcg gctcgcaccg aacagcggtg ggcgatcgcg gaagttcgcc
      481 tcgaggtaac gcaggatcgc cgccgagtcg aacagcacga cgtcgccgtg ttgcatcacc
      541 ggggtcagcc gctggcccga caggcgcagg atctcgctac gatcggcggg gtcgatcgca
      601 cggaacgcgt acgggatccg cttgtagccc agcgccacgc ggaccttgat gttgttcgac
      661 gtctcgaagc cgtggatcgt cagatcggtc gtcatcggtc gacacctccg tccggtcgcc
      721 gcggcccgga tcgtgatcga gtgagccgat tctacacggg cgagttacac ttgcgccgat
      781 gaaccgatac accgtcttgc tgctgatggg ctgcgtggcc tgcgcgacgc ccgaaacccc
      841 gatccacgag cgcgagatcg tcgacctgac ctggacgctc gacgagacga cgatcgcttg
      901 gccgacctct cccggtttca cgctcgagac gcagttcgac gggatcaccg acggtggctg
      961 gtactacctg tcgcacatct accacggacc ggagcacggc ggcacgcata tcgacgcgcc
     1021 gatccacttc cacgagggac gcgacacgac cgacgagatc ccgctgaccc ggctgatcgg
     1081 gcccggggtc aagatcgatc tccgtcacca agcggccgac gatcgtgatt accgggtcgg
     1141 gctggccgac ttcgagcgat gggagcgggc ccacggtgag ctacccgagg gtgcgatcgt
     1201 cctgctcgac accggtttcg ccgactactg gcccgaccgg cggcgctaca tgggtacgga
     1261 cggcaggggg caggaggcgg tcgccgatct gcgttttccc ggcctcgacc cggcgacggt
     1321 cgactggctc gtcggtacga agaagatccg cgcgatcggc atcgacaccg ccagcatcga
     1381 ctatggccag tcgaccgact tcgccgccca cgtcgcgctg atggagcaca acgtccccgt
     1441 tttcgagaac ctggcccgac tcgaccggct tccgccgcgc gacttcaccg tcgtggcact
     1501 gccggcgaag atcggcggcg gcagcggggg tccgctccgc atcatcgctt tgctcgaagg
     1561 ctagccgggc tcgcgatcag gcggcagccg gtcgaatgtt ctggttgacg cggaagacgt
     1621 tgctcgggtc gtacttggtc ttgatccgga ccaggcgcgc gtagttcgtg ccgtaggtcg
     1681 cctgcacgcg atcctgaccc tcctccatca tgaagttgac gtaagccccg ccggcggtct
     1741 gcccgcggag cgcgtcccag tagcccttgg cccacgaggt caacttgccg gcgttcgccg
     1801 ggtcgggatc gatgccggcg atcaccatcg accagcgcac gtcgcgatag ctccaagccg
     1861 tcgcgctcgc ggccacgtcg tggaccgcgc cgtcgatcgg gtacaggtgc atcagcgaca
     1921 gcggggtcgg cgtggcgcgg ccgtgctcgg cgtgcacgtc gatcgcggta tcggtcagct
     1981 cgcggacgaa atcgcccttc cagtaccact gcagcccctt gggcaacagc gggtcgaaca
     2041 ggccctgcag catcggaaac ggcatcgcgc cggtgtggcg gaagatcggc gccggcagct
     2101 cggccagcgc gcggtcgaag gcggcctgac cctcgccggt cggccccgac caacagccga
     2161 tcagtgcgca gatccgactc agccggatct cggccgggaa cggatcggcg tcgggcaccg
     2221 tcttgagacc gcagaacaag tacatctcac gcggcgcggt ggggagccag tcgcgatacc
     2281 attccatgat ctcccgggtg tgttcggcgt cgaagaagac cggtccgccg tagatcgtgc
     2341 ggaccggttg cgcgcggaac ttgaaactgg tgacgacgcc gaagttgccg cctccgccgc
     2401 gaacggccca gaacaggtcg tcgtgctgcc gctggttgca ggtgacgaag ctgccgtcgg
     2461 ccaacaccac gtcggcctcg agcaggttgt cgatcgtcag tccaaagcgg cgcgtgaggt
     2521 aaccgtgacc gccaccgagg gtgagcccgg cgatgcccgt ggtcgagacg attcccgccg
     2581 gcacggccaa cccgaacggg tgggtcgcgt gatcgacgtc cccctgcgta caacccgggc
     2641 cgacacgtac ggtcgcgttg gcgatgtcga cgcgtctgtc gttcatcgcc gacaggtcga
     2701 gcaccaggcc gccgtcacag ctgccgagtc ccggaccgtt gtggcccccg ccgcggatcg
     2761 cgagcagcat ctcgtgctcc gccgcgaagc ggaccgccgc gatcacatcg gcgacgtcgg
     2821 tgcagtgcgc gatcgcacgc gggcgcttgt cgatcatgcc gttgtacagc gcgcggtcgt
     2881 cgtcgtagcc cggatcatcc ggctcgacga cgcgcccgcg cagcgatcct cggagcacgt
     2941 cgatcttcat cgctcgtcta cctccgtcga aggttacgcc gaaccgcggg cagacccgcc
     3001 acggcatccc gtttttttcg gacggtcctg ccggtcggcg accccgccgg cggacggtca
     3061 tcgtgtcagg tggtgcgtcg tgatccgatg ccggtaatcg aagatccgct gcagcttgcg
     3121 ctcgacgatc gacccccaga gccaccggcc gagccggccg aaccgcagcg cgtagtcgac
     3181 gcggtcttcg agccagcagg cggcggggcc gtcgggctgg accaggtgcg tgtgctccca
     3241 gcgggcgaac ggcccgcgca gctggacgtc gcgaaacatc cgatcggcct cgaagtgggt
     3301 gtgctcggcg acccaggtca tgcgcagcgg ccagcgtccg acgagcaggg tgacgcgcga
     3361 gccgatccgg tcgatcccgc ccgttcgatc gatcacctcg acacgctccc acggcggcat
     3421 cagccgctcg aacgcgtcgg cctgcatgtg ccagcggaag accgcggcgg cgggcgcatc
     3481 gatcctcgat cgcttgacga agctgtccaa ccggaccgct cagcggatca gcgctcccgt
     3541 gccctcgatc tggaacaacg tcatcggctc ggtcacgctg gccgcgtcga attccccgcc
     3601 ctgctcctcg gccaggtgct gcaggtagcc gaccgtctgc tcgagcgtca tctcgacctt
     3661 ggtcaacacg ccctcggcga tcgcgcggcg tcccttgccg tccgccggaa agacgatgac
     3721 gccgtcaccg accttgaccc gaatctcgtc cttccggccg tcctccgcca gcgtcatcca
     3781 gcatccggcc ttctggcaca cgccggtgat gaccccctcg acgcgcaccg tcttgccgac
     3841 gtaatcgccg gcgtcggcca gcaacaccga gatcgggatc gtatcgcgcc ccgatacccc
     3901 ggcgccgaac gtctcctcgc caccgccgac cgcgaccgcc ggcagaacag cgaggaccaa
     3961 caggaccggc aggaaccgca acccacgaaa tcgcatcgat cgacctcccc cgcgaagcgc
     4021 cgttcgccgg acgccattat agagaaccgg cggtcggccg tgccgggcgg tgcaggagcg
     4081 cttcggtccg gttgccggcc ggccgccgcg gcagcgcgag cgttccttcg atcgccgccg
     4141 cgatcgcgcg atgccccgcg gcgtcgaagt gcagcacgtc gatccggccg gcctcgggtc
     4201 cgtcggcgta cgggccgaag tgcgagcgat ccaacagcga cgacgcatcg agcaggagaa
     4261 ggccgtggcg gtcgctcgcc cggtcgaccg tctcgcggaa ctgccggtag aactcgggcg
     4321 gcgtataggc cggttggccg gggtcctggc ggaacggtgt cagcagcacg agcacctccg
     4381 ccgacgaggc ggcggcgatc gcccccagcg aatcgacgaa gg
//
LOCUS       DASPGN010000159        26650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_261646, whole genome shotgun sequence.
ACCESSION   DASPGN010000159 DASPGN010000000
VERSION     DASPGN010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 26650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26650
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261646"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VD788_04430"
     CDS             complement(<1..386)
                     /locus_tag="VD788_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HXV75543.1"
                     /translation="MAERRPRRPTTPVADAPAFSKVLVANRGEIAVRVIRALHEAGIR
                     SVAVYSDADRDALHVRFAHEAYPIGPPPPLESYLDIDRVIDTARRAGAEAIHPGYGFL
                     SENADFAQACADAGVVFVGPPPAAMRA"
     gene            complement(394..1944)
                     /locus_tag="VD788_04435"
     CDS             complement(394..1944)
                     /locus_tag="VD788_04435"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXV75544.1"
                     /translation="MADPRLEQMRRRYDGARLGGGEERIAKQHGAGKLTARERVTALL
                     DPGSFQELDALVVHRSRDFDMDRNRIPGDGVVTGHGRVDGRPVVVFAQDFTVFGGSLS
                     ETFAEKVCKVMDLAMKTGVPVIGLNDSGGARIQEGVVSLAGYADIFLRNTLASGVVPQ
                     ISAIMGPCAGGAVYSPAITDFTVMVRDTSYMFITGPDVIAEVTHEQVTKEALGGALTH
                     NSTSGVAHFAAADDRDALWLIRELLSYMPSNNMEDPPQRASDDPDDRTDEQLNSVVPD
                     NPNQPYDIKRVIESVVDDGRFLEVHEHFARNIVVGFGRLGGKSVGVVANQPAVLAGVL
                     DINASVKGARFVRFCDCFNIPLITFEDVPGFLPGTDQEYRGIILHGAKLLYAFAEATV
                     PKLTVITRKAYGGAYCVMASKHIRTDFNFAWPSAEIAVMGAEGAVKIVYRSELGAAGA
                     DAASLRQRYVDDYRDKFANPYVAAERGYVDEVIEPARTRSKLIAALRLLENKRDENPP
                     RKHGNIPL"
     gene            complement(1952..2605)
                     /gene="fsa"
                     /locus_tag="VD788_04440"
     CDS             complement(1952..2605)
                     /gene="fsa"
                     /locus_tag="VD788_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HXV75545.1"
                     /translation="MKFFIDTANIDEIREANALGVLDGVTTNPSLVAREGKDFHSRLR
                     EIVSVVNGPISAEVTSTDPDTMLPEAHELAKISPNIVVKVPITAAGLKVCRKLRKDGI
                     RVNVTLCFSPAQALVAAKAGASYISPFIGRLDDVSGDGMELIRQIRAIYDNYGFETEI
                     LAASIRHPMHVVEAALAGADVATMPLNVIHQLLQHPLTDIGQEKFLADFATLKKTKA"
     gene            complement(2602..5088)
                     /gene="gyrA"
                     /locus_tag="VD788_04445"
     CDS             complement(2602..5088)
                     /gene="gyrA"
                     /locus_tag="VD788_04445"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXV75546.1"
                     /translation="MEPKENRTPINIEEEMKRSYLDYAMSVIVGRALPDVRDGLKPVH
                     RRVLYAMHETGNTADKAYRKSARTVGTVIGRYHPHGDAAVYDTIVRLAQDFSMRYPLV
                     DGQGNFGSVDGDPPAAMRYTEIRLAKLAHEMLRDIERETVDFIPNYDGSEEEPVLIPA
                     GFPNLLVNGSSGIAVGMTTNIPPHNLGELVDAIKLLVANPGATLEQLMQHVPGPDFPT
                     AGQIQGVAGIREAYATGRGRIRIRAKADIEPHPGRKDREVIIVRELPYQVNKSQLIEE
                     TADLVRDKRIEGISDIRDESDRDGIRLVIELKRGENAGVILNQLFKYTKLQTTFGVIN
                     LALVNGRPQQLTLKQMLRHFIDFRREVVVRRTIFDLRKAEERAHILEGLKIAIDNLDE
                     VVALIRASRTPPEAKEALRLRFKFSDRQAQAILDMRLQRLTGLERQKIVDEYEETLKL
                     IAKLQGILASQPLQLKIVVDELETIKQQFGDPRRTEIVPIADEIGIEDLIEERDMVIT
                     VSTTGYIKRTPLSTYEAQNRGGKGRIGMRTREEDAVRDLFVASTHDYILVFTSAGKVH
                     WLKVHEIPEVGSAGRGKPVVNLIQIMPDEKPRAMISVREFDDEHYVVLATRRGFVKKT
                     PLSAFSRPRANGIIAVTIEQDDDVLSAGLSSGADEIFMATARGQSIRFAESDVRPMGR
                     NARGVIGIRMHERDTCVGMLVLTGNRDILTVSANGYGKRTALDEYRRQGRGGSGTINM
                     RASERNGDVVAALEVAEDDHLLLITAKGKIIRMSVAGISRIGRATQGVRVIQLDEDDV
                     VVSAIRAAEDDTPGADGEQTDDNPNERGDA"
     gene            complement(5129..7570)
                     /gene="gyrB"
                     /locus_tag="VD788_04450"
     CDS             complement(5129..7570)
                     /gene="gyrB"
                     /locus_tag="VD788_04450"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012535740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HXV75547.1"
                     /translation="MGDDKDLSEGATATPTVEPEPNYDASKIKVLEGIEAVRKRPAMY
                     IGSTGDTGLHHLVWEVVDNSVDEAQAGHADRIDVTIHIDNSVTVIDNGRGIPVDIHET
                     EKIPAAEVVMTKLHAGGKFENDAYKVSGGLHGVGISVVNALSERVDLEIWRDGKTYEQ
                     AYERGKPADRFRETGVTNRRGTKVTFKPDPQIFEALIFSFETMSTRLRELAFLNKGLE
                     IVLRDERGEKVRDQVFHYEGGIVEFVQHLNRARQVLHDPVYVDGESNGTLLEFALQWN
                     DSYAETIHSFANSINTVEGGTHLAGFKAALTRTINSYLSASGMKKDKDMALSGEDCRE
                     GLTAVVSIKISKPQFEGQTKTKLGNSEVKGAVEAIVNDKLGAYLEQNPQAAKRIVMKS
                     VDAARAREAARRARELTRRKGALESGSLPGKLADCQERDPDKCELYLVEGDSAGGSAK
                     QGRDRRFQAILPLRGKILNVEKARFDKMLGHEEIRTIIAALGTGIGQDEFDTSKLRYG
                     RILIMTDADIDGAHIRTLLLTFFYRQMRPLIEKGHVFIAQPPLYKVSRGKEELYMAND
                     QELSAFVIRKATDEKTVRLPGNGASYAGKELNHLLHALIDYDRHLKSLERMGADRRTV
                     ELLLRERFAERAHFETPDRLEGLVDAIEKLGHRVVTTARDEEHGLYEMLVRSGTHGQR
                     EFRVGLELVQTVEFRQLTRLLPDVSKLDHDNLIVVTGKEQRELDSKEALLAHLLEAGK
                     KGLSIQRYKGLGEMNPGQLWETTMDPARRRVLEVRIEDAAEADMLFSVLMGDAVEPRR
                     QFIEQNALEVQNLDI"
     gene            complement(7684..8799)
                     /gene="recF"
                     /locus_tag="VD788_04455"
     CDS             complement(7684..8799)
                     /gene="recF"
                     /locus_tag="VD788_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HXV75548.1"
                     /translation="MWLTTLEADRLRNLRAVRLSLGSGLTVIAGRNGEGKSSLLEAVY
                     LLSTGRSFRTRKAEELIAWSGGPLRVAGVHHGRLGPTEIKVILDGAERRLIVDGVERD
                     QESFLGRLDVVDLTGERMQVLRGAPEERRRFLDRGIVGLRPSYLRSLGEYRRVLHQRN
                     ALLRRRGKGGGPGFATQLDSWDERLVRAAREIHAPRRSYAVHLGAELGETTRALFPGG
                     GELHLVYRPSPAALAETEPNDFEQTYAEALERGRPRDLALGHTCRGPHRDELAVELDG
                     VDLRRFGSAGQVRSAMIALKLGKLSRLAKERGETPIFLMDDFDSDIDEVRAEALADFL
                     RRGGFQALVATSKERMADRIGAPFTKVVMTEGTIRTV"
     gene            complement(8823..9971)
                     /gene="dnaN"
                     /locus_tag="VD788_04460"
     CDS             complement(8823..9971)
                     /gene="dnaN"
                     /locus_tag="VD788_04460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXV75549.1"
                     /translation="MEFVIRKADLVRELQTVTGVVEKRATLPILANLLLETTHDGLQV
                     GASDLEVTIRGTAKANVVREGSVTLPAAKLHEIARSLPEAEVQFKLLDRNQVAISCER
                     TRYRIAGQARDEFPNFPEIDLDRGVRLPAKLLNGMIERVAFAITTEDPRYSLNGALVL
                     LGKGMLTLVATDGHRLAYVTKEVGAEVPGGELKAIVPRKALAEVQKMTADSAEDDRVV
                     FGKSDNQVFFVVGPYKLTSNLLEGNFPRYENVMPESSETKITIPCEDFAHAVRRVSLL
                     ASDRYGRAIRVLLSDGKLDLSSRTEMGDAQETLSIDYDGRDVEIGFNARYLLDFLGVV
                     GSPAVSLELNPGPGGGDGTAQAGDKPGQLRPEPAGDVDYRYVVMPMHL"
     gene            complement(10237..11541)
                     /gene="dnaA"
                     /locus_tag="VD788_04465"
     CDS             complement(10237..11541)
                     /gene="dnaA"
                     /locus_tag="VD788_04465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXV75550.1"
                     /translation="MIPKNTWDRVLGRIESKISRHSYKTWFKPTGFLDEDASSISISV
                     PNAWFAEWLKANYSGLIQDALRHEDRPGLQVRFSPEERELPDLPLPCRTGDGGAPAKL
                     NPKYTFETFIVSSCNQFAHAASIAVAKQPSKAYNPLYVYGGVGLGKTHLMQAVGNRIA
                     ANGDLEMTYVSSETFMNQLINAIRFERTFEFKERYRNVDVLLIDDIQFLAGKERTQEE
                     FFYTFNALYDAQKQIIITSDVPPRDIPTLEERLRSRFEWGLIADIQPPDLETKVAILR
                     KKAECEGTPVPDDVALFIATHCGSNIRELEGAHTKVVAYASMSGREPSIELARETLHE
                     FLPTASPAATIDSIMKFVSNYYNLKVAELKSKNNSPQVAFPRQVAMYLCKLRTGCSLP
                     EIGRRFGGKHHSTVIHAVKKIDNKRRNEKEFARLLDSFEQSV"
     gene            12066..12200
                     /gene="rpmH"
                     /locus_tag="VD788_04470"
     CDS             12066..12200
                     /gene="rpmH"
                     /locus_tag="VD788_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXV75551.1"
                     /translation="MKRTYQPNNRKRKKTHGFLVRMRTKGGRLVLSRRRRKGRRRLSA
                     "
     gene            12142..12555
                     /gene="rnpA"
                     /locus_tag="VD788_04475"
     CDS             12142..12555
                     /gene="rnpA"
                     /locus_tag="VD788_04475"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXV75552.1"
                     /translation="MDALSSPGAVEKGAGDSRPDQRFPRSCRLTARRQFLIVYAEGRR
                     VSSRSFTIFGLPNTTGGCRLGITVTRRLGGAVTRNRIKRRLREVFRRNRMKLTPHLDL
                     VINAHRDAAFDDDAALEREFLRAFARLARGGASPR"
     gene            12552..12797
                     /gene="yidD"
                     /locus_tag="VD788_04480"
     CDS             12552..12797
                     /gene="yidD"
                     /locus_tag="VD788_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXV75553.1"
                     /translation="MIARSLLVALLRVYQRIVSPFLPRACRFYPSCSQYAIEAIDLHG
                     AARGSWLAVKRLARCQPFSRGGFDPVPGEGRRSCCAR"
     gene            12823..14556
                     /gene="yidC"
                     /locus_tag="VD788_04485"
     CDS             12823..14556
                     /gene="yidC"
                     /locus_tag="VD788_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXV75554.1"
                     /translation="MDSEKRLIAAIAISVLILVAFSYLTPDRAPEPGPRPEPVASDTT
                     PDGPRADEPFASAEDEPASAEPGERIEAQAEETIVVDNGVFEVALTNRGGAARGWTLR
                     DYTTGDGRPLQLLPRHVEEDAPLPLSLALDDEGLAARLNRSLFRFERSALDDPAAPGE
                     RIRFSWSSGDGYAVEKVYEFRRNDYLVGVEVAVTDRGRRLPARLTVGPGFAAQEPDST
                     HSNYYYESQASWNVGGRVTRKKRGKLGEHGGFSGPVHWAGLEDQYFTALILPNDTPAT
                     VEWRQRDLAAIAVPTPGEAAAEPEPRKTPIVAVSIGERGGLLFVGPKRYELMRSIGGD
                     RQLQRAVWFSSQDWLRVIVKYIFLGLVWIHDHVANNWGLAIILATVVLRLVLFPVNQY
                     SMVSMKKTQIQMSKLQPKIKAIRNKYKKVKDAQSRQKMNEEMMALYRKEGVNPMGGVS
                     GCLPLLAQFPILIGFYNMLTVAVELRGAPFVGWVRDLSQEDPYWILPILMAVTMFAQQ
                     KLAMTKVKDPQQLQQQRVMLFMPIVFGFICLQMPSGLVLYWFVNNVLGMGQQWLVNRH
                     AGRLEAAAQKA"
     gene            14560..15516
                     /locus_tag="VD788_04490"
     CDS             14560..15516
                     /locus_tag="VD788_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HXV75555.1"
                     /translation="MVKQREFEGKDLETALEAAAAALGVEEPELDYEIVEQGRRGLFG
                     VGAKSVRIRVMPPLADAPDPLAAAELHLPPEIREAAARRERGRRESQRRESGQPRGGP
                     RRDGQGRDRKKRTGQPAGETQPSERRDGHSGRGRSSKRRRRRRDTPRESTALPRPVPV
                     VDGPPAPGSDEVVATLDRMIGMMGMELTAKALGSTNGVIVELSGDDRDLLFDKDGELL
                     SALQFLLNRMARRTWPEAGRIQLTSDGGRPAQERDEELVELTHEVAQQVESTGKPQRL
                     RPMNAYERRLVHLTIREHTGLGSRSEGNGHLKRVKIYKQDEA"
     gene            15538..16890
                     /gene="mnmE"
                     /locus_tag="VD788_04495"
     CDS             15538..16890
                     /gene="mnmE"
                     /locus_tag="VD788_04495"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXV75556.1"
                     /translation="MVAVSTPPGRGGIACLRLSGERAGEVAGRIFRPAARAGGPRPGG
                     PPAFGRFLDRGGTPLDHGFLVLFRPEAAFTGESTAELWTHGSPAVLAELLDAACAAGA
                     EAAGPGEFTYRAVRNGRIDLTRAEAIRDLIDARTAHQARIAFAQAEGAVARHVAPLRE
                     RLEEWIARGEAAVEFVDEAETHLERGRFRDAISRIASDCRELLDGFRRGRVFRDGASL
                     AIVGRPNVGKSSLFNRLLARERAIVTEVAGTTRDTLEEHLDIGGVPVRLVDTAGLRSV
                     DDPIESEGVRRAHVARAAADLVLLVLDGSRAPEADELEALRRIEAGGERPTLVVANKS
                     DLVAAPDPALSAVRGLLAVSALSGDGIAELCARLRDEIVGTSVVEDPIVTDSRHATAL
                     ERAEAWLNRAVEAVDRGLSEELVLEDLRAAIRELGEIGGEFTDEALYDRIFSTFCIGK
                     "
     gene            16901..18757
                     /gene="mnmG"
                     /locus_tag="VD788_04500"
     CDS             16901..18757
                     /gene="mnmG"
                     /locus_tag="VD788_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HXV75557.1"
                     /translation="MSYDVIVVGAGHAGCEAAIAAARVGCATAVITLRRDAVARMSCN
                     PAIGGLAKGHIVREIDALGGVMGRVADVCGIQFRLLNRSRGPAVRGPRAQQDKAAYHL
                     AMLDEVLGTPGLDLVEGEVAALIVDGRAVRGVRLVDGRELRATRVVVTTGTFLRGLMH
                     TGAERTPGGRVGEAPARSLSDDLRRSGFSLGRFKTGTPPRLARESVDLDRFEAQPGDS
                     VPTFFSETTSGTTLPQVPCHIAYTNHAVHEIVRANLERSPMFNGTIRVKGPRYCPSLE
                     DKVYRFGDRESHTLYLEPEGLTSDLLYLNGFSTSMPAEVQLRMVRAVRGLEDCEVVRA
                     GYAVEYDYVDPRELKPTLETKRVDGLYLAGQINGTTGYEEAAGLGLIAGINAALEAQR
                     RPPLVLRREEAYIGVMIDDLVTRGTSEPYRVFTSRAEFRLLLGVDSASRRLSPHGQAI
                     GLLPAARAEATRLRWERIDAAVERLERERWVPGGTDALARTGVAIDGPVSSADLLRRP
                     GVEVATLAPASVVLAPLTEHERRVVAETIKYAGYVERQRREAARVARAGARRIPAALC
                     YRELSGLSNELVEKLEAVRPETLGRASRIDGMTPAALSLLAAHVERHAAVRP"
     gene            18754..19410
                     /locus_tag="VD788_04505"
     CDS             18754..19410
                     /locus_tag="VD788_04505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HXV75558.1"
                     /translation="MTDGPDEPSISRTVARIAAGLGLSLDEAARSRIVRHASRVLGAG
                     PELRLTAICDPRAFLARHVGESLEGASLIDPATRGAMLDLGSGNGYPGLPIAAARSGL
                     TPYLAEASRKKSAFLRSVIEVDFPGAVVLDRQIQRPADLPADVRFAIIVSRAMGNWER
                     VVPRLVPCLVPGGRVLVWAGPAMESVSRRATWSRLRPSGRRALTGRERSWIWAFDTVM
                     "
     gene            19552..20448
                     /locus_tag="VD788_04510"
     CDS             19552..20448
                     /locus_tag="VD788_04510"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV75559.1"
                     /translation="MGRIIAIVNQKGGVGKTTSAVNLAAALAIAERPILLVDADPQAN
                     TTRAIGFAADPERPTLYQVLIGDFPIEETTIQSPDLPHLSLLPADSDLVGAEVELVEA
                     DRREFRLKERIEPLRNRFDFIFIDCPPSLGLLTLNALVAADSVIIPVQCEYLALEGIS
                     ALTRTIEGVRAALNPTLEIDGVLMTMFDERTNLARQVVDEVRGVFGDRVFETLIPRNV
                     RLGEAPSYGKPIFLYDVRSKGSEAYFNLAKEFVKDEAKGVGQRSAQSDTGAAAARPEE
                     PERNRPGERHRPPPDRPRSDLA"
     gene            20438..21208
                     /locus_tag="VD788_04515"
     CDS             20438..21208
                     /locus_tag="VD788_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXV75560.1"
                     /translation="MWPNPAQPRTSFDEERLDELARSIKNQGVLQPIVVRPGKDGAYE
                     LIAGERRWRAAQMAGLLKIPAVVRETGDSQLRELALIENLQRDDLDPIEEAAAYQALI
                     DEADLTQQDVADRVGKSRATVANALRLLNLPAEVQQMIRDGQLSAGHAKALLGLASST
                     GQIELARRIVSEGLTVRSVESRLRRSASERPAPAPAEPRRDPNVVAAEQQLQKTLGTK
                     VRILQRPSGAGRLEIHFFSGEELERVFELLSGTARGRG"
     gene            21749..22291
                     /gene="atpH"
                     /locus_tag="VD788_04520"
     CDS             21749..22291
                     /gene="atpH"
                     /locus_tag="VD788_04520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HXV75561.1"
                     /translation="MKDRKLALRYARALLSALHDPAILEKTDAFLAALARALEGSAEL
                     RSALLDPAVPRGVRKRVLAAIAERNGLPREVRNFLATLTDNNRISALASIATVFHEEL
                     EKKMGVVPAELITAAPVGDELLTRAQATLERVTGRRVRLTSRVDATLIGGAVTRVGST
                     VYDGSLRRQIDQLRRKMAEG"
     gene            22317..23879
                     /gene="atpA"
                     /locus_tag="VD788_04525"
     CDS             22317..23879
                     /gene="atpA"
                     /locus_tag="VD788_04525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HXV75562.1"
                     /translation="MEIKVDEISKIIRQQIEGYDKQTDLAEVGTVISVGDGIGRIYGL
                     DRVMAGELLELPHDVMGMALNLEEDNVGAVLLGETVRIKEGDQVRRTKRIMQVPAGDA
                     MLGRVVDPLGKPLDGKGPIATNASMTIERIAPGVVHRKPVTEPMQTGLKTIDSMTPIG
                     RGQRELIIGDRQTGKTAIAVDTIINQKGQDVVCIYVAIGQKQSTIAQLVKTLEERDAM
                     SYTIVVAASASSPAPLQYLAPYGGCAMGEYFQFHGADGKPASSNNPGRHVLCIYDDLS
                     KHAAAYREISLLLRRPPGREAFPGDVFYLHSRLLERASKLNADNGGGSLTALPIIETQ
                     AGDISAYIPTNVISITDGQIFLESDLFYAGVRPAMNVGLSVSRVGGNAQVKAMKQVAG
                     TLRLSLAQFRELEAFAQFGSDLDKATQAQLNRGRRLVEILKQGQYQPLPVAKQILVIF
                     AAASGYLDNVPVEACRDFERDLYQSFDMNHPGLLAQIADKSGLTDERKEAIVKVVKEV
                     ADRYRSAAPVAG"
     gene            23896..24753
                     /gene="atpG"
                     /locus_tag="VD788_04530"
     CDS             23896..24753
                     /gene="atpG"
                     /locus_tag="VD788_04530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HXV75563.1"
                     /translation="MAKTQDLKRRIRSIRNTMQLTRAMKMVSAAKLRRAQERIMRSRP
                     YVRRTRAILASVAARANPELNPLLQQREDRRVELVVLTGDKGLCGSFNAGILRRAEGH
                     AHELVGRDLRFTAIGKRGRDHFRRRNIGLVGTWADVFRDVQFPLAAEIADGLIERYVG
                     REVDAIYLVYNEFKSAIQAQPVVVRLLPIEITDLGSTDGGEDYIYEPEPQQLLAALLP
                     HYVQQLVFQALLESVAAEHAARMTAMDSATKNAGELVDSLTLTMNRVRQASITTEIIE
                     VVSGAQGQG"
     gene            24770..26215
                     /gene="atpD"
                     /locus_tag="VD788_04535"
     CDS             24770..26215
                     /gene="atpD"
                     /locus_tag="VD788_04535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXV75564.1"
                     /translation="MSKIGKVVQIIGPVLDIEFDDEHLPAIYNAVRIRDDGSESGTKI
                     DIIAEVEQHLGENRVRCVAMEPTDGMVRGMSAEDLGEPIKVPVGEATLGRVLNVIGNP
                     VDELGPVEAEDHWPIHREAPAFDEQDTSIEMFETGIKVIDLLEPYLKGGKTGLFGGAG
                     VGKTVLIMELIHNVALKHGGFSVFGGVGERTREGNDLWLEFQETGVINIAEPSKSKAA
                     LIYGQMTEPPGARLRVALSALTVAEYFRDVKHQDVLLFIDNIFRFTQAGSEVSALLGR
                     MPSAVGYQPTLASEMGELQERITSTKTGSITSVQAIYVPADDYTDPAPATTFAHLDAS
                     TNLSRQIANLGIYPAVDPLASTSRILDPQILGENHYDTARAVQQVLQRHRELQDIIAI
                     LGMDELSDDDKLTVARARKIQRFFSQPCHVAEQFTGMPGRYVKVEDTIRGFREIVDGK
                     HDQLPEQAFLYVGTIEEALEKAEKIRRETAA"
     gene            26228..26632
                     /locus_tag="VD788_04540"
     CDS             26228..26632
                     /locus_tag="VD788_04540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HXV75565.1"
                     /translation="MADKLKLEVVTPDHSVLDQEVDEVVLPSVEGYFGVLPGHAPLLA
                     QLDVGEVSYRVGKERRFLAISGGYAEVLRTGVSVLARTAERADEIDVDRARRAKDRAE
                     SQLKQNLALDDLRRAEVKLKRAISRLSVHDRS"
BASE COUNT         4230 a         8943 c         9033 g         4444 t
ORIGIN      
        1 gcgcgcatcg ccgccggcgg cggaccgacg aagacgacgc ctgcgtcggc gcaggcctgc
       61 gcgaagtcgg cgttctccga caggaagccg taccccgggt ggatcgcctc ggcccccgcg
      121 cgcctggccg tgtcgatcac gcggtcgatg tcgaggtagg actcgagcgg cgggggcggt
      181 ccgatcgggt aggcctcgtg cgcgaaccgc acatgcagcg cgtcgcggtc ggcgtcggag
      241 tagacggcga ccgaacggat acccgcctcg tgcagggcgc ggatgacgcg caccgcgatc
      301 tcgccgcggt tggccaccag caccttgctg aacgcgggtg cgtcggcgac gggcgtcgtc
      361 ggtctgcggg gtcgtcgctc ggccatgacc accctagagc gggatgttgc cgtgcttgcg
      421 cggcggattc tcgtcccgct tgttctcgag caggcgcagc gccgcgatca acttcgaacg
      481 cgtgcgcgcc ggttcgatca cctcgtcgac gtacccgcgt tcggcggcga cgtaggggtt
      541 ggcgaacttg tcgcgatagt cgtcgacgta tctctggcgc aacgatgcgg cgtccgcccc
      601 cgccgcgccc agctcgctgc ggtagacgat cttgaccgcg ccctcggcgc ccatcaccgc
      661 gatctccgcg ctgggccagg cgaagttgaa gtcggtgcgg atgtgcttcg atgccatcac
      721 gcagtacgca ccgccgtacg ccttgcgggt gatcaccgtc aacttgggta cggtcgcctc
      781 ggcgaaggcg tacagcagct tggcgccgtg caggatgatg ccacggtact cctggtcggt
      841 ccccggaagg aagccgggga cgtcctcgaa ggtgatcagc gggatgttga agcagtcgca
      901 gaagcggacg aaccgtgcgc ccttgaccga ggcgttgatg tcgagcaccc cggccagcac
      961 cgccggctga ttggccacga cgccgaccga cttgccgccc agacgcccga agccgacgac
     1021 gatgttgcgc gcgaagtgct cgtgcacttc gaggaagcgc ccgtcgtcga ctaccgattc
     1081 gatcacgcgc ttgatgtcgt acggttgatt cgggttgtcg gggacgaccg agttcagctg
     1141 ctcgtcggtg cggtcgtcgg ggtcgtcgct ggcgcgctgc ggtgggtctt ccatgttgtt
     1201 cgacggcatg taggacaaca gctcgcgaat cagccacagc gcgtcgcggt cgtcggccgc
     1261 ggcgaagtgc gcgacacccg aggtcgagtt gtgggtcagc gcgccgccca gcgcctcctt
     1321 ggtcacctgc tcgtgcgtca cctcggcgat cacgtccggt ccggtgatga acatgtacga
     1381 ggtgtcgcgc accatgacgg tgaagtcggt gatcgccggc gagtagacgg cgccgccggc
     1441 gcacgggccc atgatcgccg agatctgcgg caccacgccc gaggccagcg tgttgcgcag
     1501 gaagatgtcg gcgtagcccg ccagcgagac caccccttcc tgaatccgcg cgccgcccga
     1561 gtcgttgagc ccgatcaccg gcacgccggt tttcatcgcc aggtccatca ccttgcagac
     1621 cttctcggcg aaggtctcgg acagcgaccc gccgaaaacg gtgaagtcct gggcgaagac
     1681 cacgaccggc cggccgtcga cccgcccgtg gccggtgacc acgccgtcgc ccgggatgcg
     1741 gttgcgatcc atgtcgaagt ctcgactgcg gtggacgacg agcgcgtcga gctcctggaa
     1801 cgaccccggg tcgagcagcg cggtgacacg ctcgcgggcc gtcagcttgc ccgccccgtg
     1861 ctgcttggcg atccgttcct cgcccccgcc gaggcgtgcg ccgtcgtagc gccgacgcat
     1921 ctgttccagt cgtgggtcgg ccatcgaccg gctaggcttt ggtcttcttc agcgtggcga
     1981 agtcggcgag gaacttctcc tgaccgatgt cggtcagcgg gtgctgcagc agctggtgga
     2041 tcacgttgag cggcatcgtc gcgacgtccg cgccggcgag cgcagcctcg acgacgtgca
     2101 tcggatggcg gatgctggcc gcgaggatct ccgtctcgaa gccgtagttg tcgtagatcg
     2161 cgcggatctg gcggatcagc tccatcccgt cgcccgacac gtcgtccaag cgaccgatga
     2221 acgggctgat gtagctggcg ccggccttgg ccgcgaccag cgcctgggcc ggcgagaagc
     2281 agagcgtgac gttgacgcgg atcccgtcct tgcgcagctt gcggcagacc ttcagcccgg
     2341 cggccgtgat cggcaccttg acgacgatgt tggggctgat cttcgccagc tcgtgcgcct
     2401 cgggcaacat cgtgtcggga tcggtcgagg tcacctcggc cgaaatcggc ccgttgacca
     2461 cgctgacgat ctcgcgcagg cgcgagtgga agtccttgcc ctccctcgcc accagcgagg
     2521 ggttggtcgt cactccgtcg agcacgccga gcgcgttggc ctcgcggatc tcgtcgatgt
     2581 tcgcggtgtc gataaagaat ttcatgcatc acctcgctcg ttgggattgt cgtccgtctg
     2641 ttcgccgtcc gcgcccggcg tatcgtcctc ggcggcgcga atcgccgaca ccaccacgtc
     2701 gtcctcgtcg agctgaatca cgcgcacgcc ctgcgtggcg cggccgatgc ggctgatgcc
     2761 cgcgacgctc atgcggatga tcttgccctt ggcggtgatc agcagcaggt ggtcgtcctc
     2821 ggccacctcg agcgccgcga cgacgtcgcc gttgcgctcg gacgcccgca tgttgatcgt
     2881 tccggagccg ccgcggccct gccgacgata ctcgtcgagc gcggtgcgct tgccgtagcc
     2941 gttggcgctg acggtgagga tgtcgcggtt gccggtcagc accagcatgc cgacgcaggt
     3001 gtcccgctcg tgcatgcgga tgccgatcac gccgcgggcg ttgcgaccca tcggccgcac
     3061 gtcgctctcg gcgaagcgga tcgattggcc gcgggcggtg gccatgaaga tctcgtcggc
     3121 gcccgacgac aacccggccg aaagcacgtc gtcgtcttgc tcgatcgtga cggcgatgat
     3181 gccgttggcg cgcgggcgcg agaaggccga cagcggagtc ttcttgacga agccgcgacg
     3241 ggtggcgagc acgacgtagt gctcgtcgtc gaactcgcgg accgagatca tcgcccgcgg
     3301 cttctcgtcg ggcatgatct ggatcaggtt gacgaccggc ttgccgcggc cggccgagcc
     3361 gacctccgga atctcgtgca ccttgagcca gtggaccttg cccgccgagg tgaagaccag
     3421 gatgtagtcg tgggtcgagg cgacgaacag gtcgcgcacc gcgtcttcct cgcgcgtgcg
     3481 catgccgatg cggcccttgc caccgcggtt ctgcgcttcg taggtcgaca gcggcgtacg
     3541 cttgatgtaa ccggtggtcg acaccgtgat caccatgtcg cgctcttcga tcaggtcttc
     3601 gatgccgatt tcgtcggcga tcgggacgat ctcggtgcga cgcgggtcgc cgaactgctg
     3661 cttgatcgtc tccagctcgt cgacgacgat cttcagctgc aacggctggc tggccaggat
     3721 gccctgcagc ttggcgatca gcttcagcgt ctcctcgtac tcgtcgacga tcttctgccg
     3781 ttcgagcccg gtcagccgtt gcaggcgcat gtcgaggatc gcctgcgcct gacggtcgct
     3841 gaacttgaag cgcaggcgca gcgcctcctt cgcctcgggc ggcgtcctcg acgctcggat
     3901 cagcgccacc acctcgtcga ggttgtcgat cgcgatcttc aacccttcga ggatgtgcgc
     3961 ccgttcctcc gccttgcgca ggtcgaagat cgtgcggcgg acgaccacct cgcggcggaa
     4021 atcgatgaag tgcctcagca tctgcttgag cgtcagctgc tgcggtcgtc cgttgaccag
     4081 cgccaggttg atcacgccga aggtggtctg cagcttggtg tacttgaaca gctggttgag
     4141 gatcacgccg gcgttctcgc cgcgcttcag ctcgatcacc agccggatgc cgtcgcggtc
     4201 cgactcgtcg cggatgtcgc tgatgccctc gatccgcttg tcacgcacga ggtcggccgt
     4261 ttcctcgatc agttgcgact tgttgacctg atacggcagc tcgcggacga tgatcacctc
     4321 gcggtccttg cgcccggggt gcggctcgat gtcggccttg gcacggatgc ggatgcgccc
     4381 gcgaccggtg gcgtaggcct cgcggatgcc ggccacgccc tggatctggc cggccgtcgg
     4441 gaagtcgggg cccggcacgt gctgcatcag ctgctcgagc gtcgcgcccg ggttggcgac
     4501 caacagcttg atcgcgtcga ccaactcgcc gaggttgtgt ggcgggatgt tggtcgtcat
     4561 gccgaccgcg atgccggacg agccgttgac cagcaggttg gggaagccgg ccgggatcag
     4621 caccggttcc tcttccgagc cgtcgtagtt ggggatgaag tcgaccgtct ccctctcgat
     4681 gtcgcgcagc atctcgtgcg cgagcttggc cagacggatc tcggtgtaac gcatcgccgc
     4741 cggcggatcg ccgtcgaccg agccgaagtt gccttgcccg tcgaccagcg ggtagcgcat
     4801 cgagaagtct tgggccaggc gcacgatcgt gtcgtagacc gcggcgtcgc cgtgcgggtg
     4861 gtagcggccg atcaccgtgc cgaccgtgcg cgccgacttg cggtaggcct tgtccgcggt
     4921 gttgccggtc tcgtgcatcg cgtacagcac gcggcgatgc accggcttga gcccgtcccg
     4981 cacgtcgggc agcgcgcgtc ccacgatcac gctcatcgcg taatcgaggt aggaccgctt
     5041 catctcctct tcgatgttga tcggagtgcg attctctttc ggttccatca acggcctcta
     5101 gctcgagcgc ggattcgacc ggcgaggttc agatgtcgag gttttgcact tcgagggcgt
     5161 tctgctcgat gaactgccgg cgcggctcga ccgcgtctcc catcagcacc gagaacagca
     5221 tgtcggcttc ggccgcatcc tcgatgcgca cctcgagcac ccgccgccgt gcggggtcca
     5281 tcgtcgtctc ccacaactgg ccggggttca tctcgccgag acccttgtag cgctggatcg
     5341 acaggccctt cttgcccgcc tcgagcaggt gggccaacaa cgcttccttg gagtcgagct
     5401 ctcgttgttc cttgccggtc acgacgatca ggttgtcgtg gtccagcttc gacacgtcgg
     5461 gcaacagccg cgtgagctgg cggaactcga ccgtctgcac cagctcgaga ccgacgcgga
     5521 actcgcgctg gccgtgcgtg ccggaacgga cgagcatctc gtacagcccg tgctcctcgt
     5581 cgcgcgcggt cgtgaccacg cggtggccga gcttctcgat cgcgtcgacc agcccctcga
     5641 gccggtcggg cgtttcgaaa tgcgcgcgct cggcgaaccg ctcgcgcagc agcagctcga
     5701 cggtccggcg atcggcgccc atccgctcga gcgacttgag gtggcggtcg tagtcgatca
     5761 gcgcgtgcag caggtggttc agctccttgc cggcgtagct cgcaccgttg cccggcaggc
     5821 ggacggtctt ctcgtcggtc gccttgcgga tgacgaaggc cgacaactcc tggtcgttgg
     5881 ccatgtacag ctcttccttg ccccgcgaga ccttgtacag cggcggttgc gcgatgaaca
     5941 cgtgcccctt ctcgatcaac ggccgcatct gacggtaaaa gaaggtgagc aacaacgtgc
     6001 ggatgtgcgc accgtcgatg tcggcgtcgg tcatgatcag gatccgcccg tagcgcagct
     6061 tcgaggtgtc gaactcgtcc tgaccgatgc cggtgccgag cgcggcgatg atcgtgcgga
     6121 tctcctcgtg gcccagcatc ttgtcgaacc gggccttctc gacgttgagg atcttgccgc
     6181 gcagcggcag gatcgcctgg aaccggcggt ctcgtccctg cttggccgag ccgcccgccg
     6241 aatcaccctc gaccaggtac agctcgcact tgtcggggtc gcgctcctga cagtcggcca
     6301 gcttgccggg cagcgacccc gactcgagcg cccccttgcg tcgggtcagc tcgcgcgccc
     6361 gccgcgcggc ctcgcgcgcc cgcgcggcgt cgacgctctt catcacgatc cgcttggcgg
     6421 cctgcgggtt ctgctcgagg tacgcgccga gcttgtcgtt gacgatcgcc tcgacggcgc
     6481 ccttgacctc cgagttgccg agcttggtct tggtttggcc ctcgaactgc ggcttcgaga
     6541 tcttgatcga gacgacggcg gtcagcccct cccggcaatc ctcgccggac agcgccatgt
     6601 ccttgtcctt cttcatcccg gaagcggaca ggtacgagtt gatcgtccgc gtcagcgcgg
     6661 ccttgaaccc ggccagatgc gtcccgccct cgaccgtgtt gatcgagttg gcgaacgagt
     6721 ggatcgtctc ggcgtaggag tcgttccact gcagcgcgaa ctcgagcagc gttccgttcg
     6781 actcgccgtc gacgtagacc ggatcgtgca gcacctgccg cgcccggttg aggtgctgga
     6841 cgaactcgac gatgccccct tcgtagtgga agacctgatc gcggactttc tcgccgcgct
     6901 cgtcgcggag gacgatctcg agacccttgt tgaggaaggc cagttcgcgc aggcgcgtgg
     6961 acatcgtctc gaagctgaag atcagcgcct cgaagatctg cggatcgggc ttgaacgtga
     7021 ccttggtgcc gcgtcggttg gtgacaccgg tctcgcggaa gcggtcggcc ggtttgccac
     7081 gctcgtaggc ctgctcgtag gtcttgccgt cgcgccagat ctcgagatcg acgcgctccg
     7141 acagcgcgtt gaccaccgag atgccgacgc cgtgcagccc gcccgatacc ttgtacgcgt
     7201 cgttttcgaa cttgccgccg gcgtgcagtt tggtcatcac gacctccgcc gccgggatct
     7261 tctccgtctc gtggatgtcg accgggatgc cccggccgtt gtcgatcacg gtcaccgagt
     7321 tgtcgatgtg gatcgtcacg tcgatccggt cggcgtggcc ggcctgcgcc tcgtcgaccg
     7381 agttgtcgac gacctcccac accaggtggt gcagacccgt gtcgcccgtc gagccgatgt
     7441 acatcgccgg acgcttgcgg accgcctcga tgccctcgag aaccttgatc ttgctggcgt
     7501 cgtagttcgg ttccggctct acggtggggg tcgcggtggc gccttcggaa aggtctttgt
     7561 cgtcacccat cctgcttttc tccgttcgac cggtcccatc ggcggcttcg tcaagcccgc
     7621 cgcggacccc gattcgatcg gcctctcgtc gccctttggt gtgccccgcg caggtcggtg
     7681 gtgtcagacc gtgcggatcg tcccctcggt catcacgacc ttggtgaacg gcgcaccgat
     7741 ccggtcggcc atacgctcct tcgatgtcgc cactagggcc tgaaacccac cccggcgaag
     7801 gaaatccgcc agggcttcgg ctcggacctc gtcgatgtcc gaatcgaagt cgtccatcaa
     7861 gaagatgggg gtctctccac gctccttcgc gagcctggag agcttaccca acttcaaagc
     7921 tatcatagcg cttctgacct gcccagcgga cccgaaacgc ctcagatcga ccccgtcgag
     7981 ctcgaccgcc agctcgtcgc ggtgaggccc gcgacaggtg tggccgagcg ccaggtcgcg
     8041 cggccgtcca cgctcgagtg cttcagcgta agtctgttca aaatcattcg gttccgtctc
     8101 ggcgagcgcc gccggggacg gccggtagac caggtggagc tccccgccac cgggaaacag
     8161 ggcgcgcgtc gtctcgccga gctccgcacc gaggtggacc gcgtaggacc ggcgcggcgc
     8221 gtggatctcg cgggccgccc gaacgagccg ctcgtcccag ctgtcgagtt gcgtcgcgaa
     8281 ccccggcccg ccgcccttcc cgcgccggcg taacagcgcg ttgcgctggt gcaacacccg
     8341 acggtattcc ccgagcgagc gaaggtagga cgggcggagc ccgacgatac cccggtcgag
     8401 gaaccgccga cgctcttccg gcgcgccgcg cagcacctgc atccgctcgc cggtcaggtc
     8461 gacgacgtcc aaacgcccga ggaacgactc ctgatcgcgc tcgaccccgt cgacgatcag
     8521 ccgccgctcg gcgccgtcca ggatcacctt gatctcggtc ggtcccagac gtccgtggtg
     8581 gaccccggcc acgcggagcg gcccgccgct ccaggcgatc agctcctcgg ccttgcgcgt
     8641 acggaacgag cgaccggtcg acagcaggta gaccgcctcg agcaggctgc tcttgccctc
     8701 gccgttccga ccggcgatca cggtcaggcc ggagccgagc gagaggcgca ccgcgcgcag
     8761 gttgcgcagc cgatccgctt cgagcgtggt gagccacatc ggccggggcg cggcctgcgg
     8821 gtttacaggt gcatcggcat gacgacgtag cggtagtcca cgtcgccggc gggttcgggg
     8881 cggagctgac cgggcttgtc gccggcctgc gccgttccgt cgcccccgcc cggaccggga
     8941 ttcaactcga ggctgacggc cgggctgccg acgacgccga ggaaatcgag caggtaacga
     9001 gcgttgaacc cgatctcgac gtccctaccg tcgtagtcga tcgacagcgt ctcctgggcg
     9061 tcgcccatct cggtgcggct cgacagatcg agctttccgt cggacagcaa gacgcggatc
     9121 gcgcggccgt aacgatcgct cgccaacaac gagacgcggc gcacggcgtg ggcgaagtcc
     9181 tcacacggga tcgtgatctt ggtctcgctc gactccggca tcacgttctc gtagcgcggg
     9241 aagttgccct cgagcaggtt cgacgtgagc ttgtacggcc cgaccacgaa gaagacctgg
     9301 ttgtccgact tgccgaagac gacccggtcg tcctcggctg agtccgccgt catcttctgc
     9361 acttcggcca acgccttgcg cgggacgatc gccttcagct cgcccccggg gacctcggca
     9421 cccacctcct tcgtgacgta ggccaggcgg tggccgtcgg tcgcgacgag cgtcagcatg
     9481 cccttgccga gcaggaccaa cgcgccgttc agcgagtagc gcggatcttc ggtcgtgatc
     9541 gcgaacgcca cccgctcgat catcccgttg agcagcttcg ccggtagccg gacacctcga
     9601 tccagatcga tctcggggaa gttggggaac tcgtcacgcg cctggcctgc gatgcggtag
     9661 cgggtacgct cgcaggagat cgcaacctgg ttgcgatcga gcaacttgaa ttgcacttcg
     9721 gcttcgggca gcgagcgcgc gatctcgtgc agcttggccg cggggagcgt cacgctgccc
     9781 tcgcgcacga cattggcctt ggccgtcccg cggatggtca cctcgagatc gctcgcgccg
     9841 acctgcaggc cgtcgtgggt cgtctcgagc agcaggttgg ccaggatcgg caacgtggcc
     9901 cgcttctcga cgaccccggt gacggtttgt agctcgcgga ccagatcggc cttgcggatg
     9961 acgaattcca tgatttgcct cgaatcctgg attctgacct atacaccata gagaagtaat
    10021 caaaaaaacg cagtatgaag cagggacgtg gaaacggcgg aaaacgctcc aagcaccgcg
    10081 cccccggcgg tttaacccgc cggaccgtct gatgataacc ccgccgagcc cttcggaaaa
    10141 gtgggagcgc ccgtcgctcc ccgccacata tccaccgatc ccggcgtcga tccacacggt
    10201 atcgaccggt cgtccacagc gagatgtgga tcggtctcag accgactgct cgaagctgtc
    10261 gagcagcctg gcgaattcct tctcgttccg tcgcttgttg tcgatcttct tgaccgcgtg
    10321 gatcaccgtc gagtggtgct tgccaccgaa ccgccggccg atctcgggca acgagcagcc
    10381 ggtgcggagc ttgcacaggt acatcgccac ctgtcgcggg aaggccacct gcgggctgtt
    10441 gttcttcgac ttgagctcgg ccaccttcag gttgtagtag ttcgagacga atttcatgat
    10501 gctgtcgatc gtcgccgcgg gtgaggcggt cggcaggaac tcgtggagcg tctcgcgcgc
    10561 cagctcgatc gacggctcgc gaccggacat cgatgcgtag gccacgacct tcgtgtgcgc
    10621 accctcgagc tcgcggatgt tcgagccgca atgggtcgcg atgaacagcg ccacgtcgtc
    10681 gggcaccggg gtcccttcgc actcggcctt tttgcgcagg atcgccacct tcgtctcgag
    10741 atcgggtggc tggatgtcgg cgatcagacc ccactcgaac cgcgatcgga gccgttcctc
    10801 gagtgtcggg atgtcgcgcg gcgggacgtc gctcgtgatg atgatctgct tctgcgcgtc
    10861 gtacagcgcg ttgaacgtat agaagaactc ctcctgggtg cgctccttgc ccgcgaggaa
    10921 ctggatgtcg tcgatcagca gcacgtccac gttgcgatag cgctccttga actcgaacgt
    10981 acgctcgaag cggatcgcgt tgatcagctg gttcatgaac gtctcgctcg agacgtaggt
    11041 catctcgagg tcgccgttgg cggcgatgcg attgccgacc gcctgcatca ggtgcgtctt
    11101 gcccagaccg accccgccgt agacgtagag cgggttgtag gccttcgacg gttgcttggc
    11161 gaccgcgatc gacgcggcgt gagcgaactg gttgcacgac gacacgatga aggtttcgaa
    11221 ggtgtacttc gggttcaact tggcgggcgc gccgccgtca cccgtgcggc acggcagcgg
    11281 aaggtcgggc agctcgcgct cttcggggct gaaccgcacc tggagacccg gccggtcctc
    11341 gtggcgcagc gcgtcctgga tcagaccgga gtagttcgcc ttgagccact cggcgaacca
    11401 ggcgttcggc acgctgatcg agatgctcga cgcgtcttcg tcgaggaagc ccgtcggttt
    11461 gaaccaggtc ttgtacgagt ggcggctgat cttgctctcg attcgaccca gcacgcggtc
    11521 ccaggtgttc ttcggaatca cggcactcaa ggtgacctcc gagattgagg aattgtgatt
    11581 gatcgagaac cgaatcgctc gcgacgtcgt gtggacgccg gggtgccaac agattttcca
    11641 catgtgtgga aaaggcgtgt gacagctaag tgtcggccgg agcgacgctt cgcacgatcg
    11701 ctcgacggac gggtgtgaga gttatgtaaa ccgaccccgg gttggtttct aaggcattta
    11761 ttttgaatga gatagagatt gcgatgtctc gtcggagaca acacgaatcc ccgcgtccag
    11821 gttgcgacac cgtgcaggat gtctgggttc ttgaccagcc gggaacggcc ggcgaagcct
    11881 atcacaggcc cgtcggccgt cgcaatacga caaatgcatg gatttgattt tttatttatt
    11941 tgcgctcatg agcttatcaa tattattttc gtttgtggtc gacggccgtc caggcgcttt
    12001 cttgacagcc cgggggcgct tccctaacat ggctgctttg gtctccgagg caggcacggt
    12061 ttaccatgaa gcgcacgtat cagccgaaca accgcaagcg caagaagacc cacgggttcc
    12121 tggtccgaat gcgcacgaag ggtggacgcc ttgtcctctc ccggcgccgt cgaaaagggc
    12181 gcaggcgact ctcggcctga ccagcggttt cctcgatcct gccgtctgac agcgcggcgg
    12241 caattcctga tcgtctacgc cgagggccgc cgcgtgagct ccaggtcctt taccatcttc
    12301 gggctcccca acacgaccgg tggctgtcgg ctgggaatca ccgtgacgcg caggctgggg
    12361 ggtgcggtga cgcgcaaccg catcaagcgc cggctgcgcg aagtgttcag gcggaaccgg
    12421 atgaagctga cgccacactt ggatctcgtg atcaacgcac accgcgacgc ggcgttcgac
    12481 gacgacgcgg cgctcgagcg ggagttcctg cgtgcgttcg cgcggctcgc gcgcggcggg
    12541 gcgagtccgc gatgatcgcg cggtcgttgc tggtcgccct actccgggtt taccagcgta
    12601 tcgtctcgcc gttcctaccg cgtgcgtgcc gcttctaccc ttcctgttcc cagtacgcga
    12661 tcgaggcgat cgacttgcac ggcgccgcgc gagggtcgtg gctcgcggtg aagcgcttgg
    12721 cacgctgcca gccgttcagc cgtggcggat tcgatcccgt gccgggcgag ggacggcgct
    12781 cctgctgcgc ccgctaggcc gcccgaccga gacgaggacc cgatggactc cgaaaagcgt
    12841 ctgatcgccg cgatcgcgat atcggtgctg atcctcgtcg cgttctccta cttgacgccc
    12901 gatcgcgctc ccgagccggg gccgagaccg gagcccgtgg cctcggacac gacgcccgac
    12961 gggccccggg ccgacgagcc gttcgcatcg gccgaggacg agccggcgtc ggccgaaccg
    13021 ggcgagcgga tcgaggcgca ggccgaagag acgatcgtcg tcgacaacgg cgtgttcgag
    13081 gtcgcgctga ccaatcgcgg gggtgccgcc agaggctgga cgctccgcga ttacacgacg
    13141 ggggacggcc ggccgctgca gctcctcccg cgccacgtcg aagaggacgc gccgctcccg
    13201 ttgtcgctcg cgctcgacga cgaggggctc gccgcgcggc tgaaccgcag cctgttccgt
    13261 ttcgagcgga gcgcgctcga cgacccggcg gccccgggcg agcggatccg cttcagctgg
    13321 tcgtcgggcg acgggtatgc ggtcgagaag gtctacgagt tccgccgcaa cgactacctc
    13381 gtcggcgtcg aggtcgcggt caccgatcgc ggacggcgcc tgccggcgcg actgaccgtc
    13441 ggcccgggct tcgcggccca ggagcccgac tcgacccaca gcaactacta ctacgagagc
    13501 caggcgtcgt ggaacgtcgg cgggcgcgtg acgcgcaaga agcgcgggaa gctcggcgaa
    13561 cacggcggct tcagcgggcc ggtccactgg gccggtctcg aggaccagta cttcaccgcg
    13621 ttgatcctgc ccaacgacac accggcgacg gtcgaatggc gccagcggga tctggccgcg
    13681 atcgcggttc ccaccccggg cgaggcggcg gccgagcccg agccgcggaa gacgccgatc
    13741 gtcgccgtgt cgatcggcga gcgtggcggc ctgctgttcg tcggacccaa acgctacgag
    13801 ctgatgcggt cgatcggcgg ggaccgccag ctccagcgag ccgtctggtt ctcgagccag
    13861 gattggctgc gcgtcatcgt gaagtacatc ttcctcggcc tggtctggat ccacgatcac
    13921 gtcgccaaca actggggtct ggcgatcatc ctcgcgacgg tggtgttgcg gctggttctc
    13981 ttcccggtga accagtactc gatggtcagc atgaagaaga cccagatcca gatgtccaag
    14041 ctgcagccca agatcaaggc catccgcaac aagtacaaga aggtcaagga cgcccagtcg
    14101 cgacagaaga tgaacgaaga gatgatggcg ctctaccgca aggagggcgt caacccgatg
    14161 ggcggggtat cgggctgcct gccgctgctt gcccagttcc caatcttgat cggtttctat
    14221 aacatgctga ccgtggccgt ggagctgagg ggggcgccgt tcgtcggctg ggtacgcgat
    14281 ctgtctcagg aggacccgta ctggatcctg ccgatcttga tggccgtgac catgttcgcg
    14341 cagcagaagc tggcgatgac caaggtcaag gacccccagc agctccagca gcagcgggtg
    14401 atgttgttca tgccgatcgt cttcggtttc atatgtttac agatgcccag cgggctggtg
    14461 ctgtactggt tcgtgaacaa tgtgctgggc atgggccagc aatggctcgt caaccgacac
    14521 gccggtcgcc tcgaggcggc ggcgcaaaag gcctgacaca tggtcaagca acgagagttc
    14581 gaaggtaagg atctggagac ggcgctcgag gccgcggcgg ccgcgctcgg tgtcgaggag
    14641 ccggagctcg actacgaaat cgtcgagcag ggcaggcgtg gcctgttcgg cgtcggcgcg
    14701 aagagcgtgc ggatccgggt gatgccgccg ctggccgacg cgcccgaccc gctggcggcg
    14761 gccgagctgc acctgccgcc ggagattcgc gaggcagccg cccggcgcga gcgcggcaga
    14821 cgcgagtcgc agcggcgcga gtcgggtcag cctcggggcg gcccgcggcg cgacggccag
    14881 gggcgagaca ggaagaaacg caccggtcag ccggcgggcg agacccagcc gtccgaacgg
    14941 cgcgacgggc attcgggtcg gggccgttcg tccaagcgtc gccgccggcg ccgtgatacg
    15001 ccgcgcgagt cgacggcgct gccgcgaccg gttccggtcg tggacggccc gcccgccccc
    15061 ggctcggacg aggtcgtcgc gacgctcgac cggatgatcg ggatgatggg gatggaactc
    15121 accgccaagg cgttgggctc gaccaacggc gtgatcgtcg agctgagcgg cgacgaccgc
    15181 gatttgctgt tcgacaagga cggcgagctg ttgtcggccc tccagttcct gctgaaccgg
    15241 atggcgcgcc gcacgtggcc cgaggccggc cgcatccagc tcacgagcga cggcggccgt
    15301 cccgctcagg agcgggacga ggaactcgtc gagctgacgc acgaggtggc ccagcaggtc
    15361 gagagcaccg gcaagcccca gcggctccgc ccgatgaatg cgtacgagcg ccggctcgtg
    15421 catctgacca tccgcgagca caccgggctc ggctcgcgat cggagggcaa cggccacctc
    15481 aagcgcgtga agatctacaa gcaggacgag gcctgactgc acgacccggg tagcacgatc
    15541 gtcgcggtga gcacgccgcc gggacggggt ggcatcgcct gcctgcgctt gagcggcgag
    15601 cgagcgggcg aggtcgcggg tcggatcttc cggccggcgg cccgtgccgg gggccctcgt
    15661 cccggtggcc cgcccgcgtt cggccgcttt ctcgaccggg gcggtacacc gctcgatcac
    15721 ggctttctcg tcctgttccg cccggaagcc gccttcaccg gcgagtcgac ggccgagctg
    15781 tggacccacg gcagcccggc ggtgctggcc gagctgctcg acgcggcatg cgccgccggc
    15841 gccgaggcgg ccgggcccgg cgagttcacc taccgcgccg tccgcaacgg gcggatcgac
    15901 ctgacccggg ccgaggcgat ccgcgatctg atcgacgcgc gcaccgccca ccaggcgcgg
    15961 atcgcgttcg cccaggccga gggggcggtg gcgcgccacg tggcaccgct ccgcgagcgg
    16021 ctcgaggaat ggatcgcccg tggcgaggcg gcggtcgagt tcgtcgacga agccgagacc
    16081 cacctcgagc gcgggcggtt ccgcgacgcg atctcccgca tcgcctccga ctgtcgcgaa
    16141 ctgctcgacg gcttccgccg cggccgcgtc ttccgtgacg gggcgagcct ggcgatcgtc
    16201 ggccgtccga acgtgggcaa gtcgtcgctg ttcaaccgcc tgctggcgcg cgagcgcgcg
    16261 atcgtcaccg aggtcgccgg cacgacccgc gacacgctcg aggagcacct cgacatcggc
    16321 ggcgtaccgg tgcggctggt cgacacggcg ggcttgcgct cggtcgacga cccgatcgag
    16381 agcgagggcg tgcggcgggc acacgtcgcg cgcgccgccg ccgatctcgt cctgctcgtg
    16441 ctcgacggct cgcgggcgcc cgaggccgac gagctcgagg cactgcgccg gatcgaggcc
    16501 gggggcgagc gacccacgct ggtcgtggcc aacaagtccg acctcgtcgc cgcacccgac
    16561 ccggcgcttt ctgcggtccg cggactgctc gccgtttccg cgctctcggg cgacggcatc
    16621 gcggagctct gcgcgcgcct gcgggacgag atcgtcggga cgtcggtcgt cgaggacccg
    16681 atcgtgaccg acagccgaca cgcgacggcg ctcgagcggg ccgaggcctg gttgaaccgg
    16741 gcggtcgagg ccgtcgatcg cggtctgtcc gaggagctcg tgctcgagga cctgcgcgcg
    16801 gcgatccgcg agctggggga gatcggcggt gagttcaccg acgaagcgtt gtacgatcgg
    16861 atcttctcca cgttctgcat cggcaagtga ggtcgggtcg gtgagctacg acgtgatcgt
    16921 cgtcggcgcg ggccacgcag gttgcgaggc ggcgatcgcc gccgcgcggg tcggctgtgc
    16981 caccgccgtt atcacgctgc ggcgcgacgc ggtcgcgagg atgtcgtgca acccggcgat
    17041 cggcgggctg gccaaggggc acatcgttcg agagatcgac gcgctcggcg gggtgatggg
    17101 ccgggtcgcc gacgtctgcg gcatccagtt ccgactgctc aaccggagtc gcgggccggc
    17161 cgtgcgcggt ccgcgggccc agcaggacaa ggccgcctat cacctggcga tgctcgatga
    17221 ggtgctcggc acacccggtc tcgatctggt cgagggcgag gtggcggcgc tgatcgtcga
    17281 cgggcgagcg gtccgcggcg tacgtctcgt cgacgggcgc gagctccgcg cgacgcgcgt
    17341 cgtcgtgacg accgggacct tcctgcgcgg gctgatgcac accggggccg agcgcacgcc
    17401 gggtgggcgg gtcggcgagg cgccggcgcg cagcctgtcg gacgacctac ggcgctcggg
    17461 tttctcgctc gggcggttca agaccgggac gccgccccgt ctggcgcggg agagcgtcga
    17521 tctcgaccgc ttcgaggcgc agccgggcga cagcgtcccg accttcttct ccgagacgac
    17581 gagcggaacg acgctgcctc aggtcccgtg tcacatcgcg tacaccaacc acgcggtgca
    17641 cgagatcgtg cgcgccaatc tcgagcggtc accgatgttc aacggcacga tccgcgtcaa
    17701 gggcccgcgt tactgtccat cgctcgagga caaggtctac cgcttcggcg atcgggaatc
    17761 gcacacgctc tatctcgagc ccgaggggct cacgagcgac ctgctctatc tcaacgggtt
    17821 ctcgacctcg atgcccgcgg aggtccagct gcgcatggtg cgcgcagtac ggggtctgga
    17881 agactgcgag gtcgtccgcg cggggtacgc ggtcgagtac gactacgtcg acccgcggga
    17941 gctgaaaccc acgctcgaga ccaaacgggt cgacggactg tacctcgccg gccagatcaa
    18001 cggaacgacc ggctacgagg aggccgcggg tctcggtctg atcgccggca tcaacgccgc
    18061 gctcgaggcg cagcgccggc cgccgctcgt gctccggcgc gaggaggcgt acatcggcgt
    18121 gatgatcgac gacctcgtca cgcgcggcac gagcgagccg taccgcgtct tcacctcgcg
    18181 cgccgagttt cgcctgctgc tcggcgtcga ttcggcgagc cgtcgtctct cgccgcacgg
    18241 gcaagcgatc gggctcctgc cggcggcgcg ggccgaggcg acgcggctgc gctgggaacg
    18301 gatcgacgcc gccgtcgagc gactcgagcg cgagcgctgg gtcccgggcg gcaccgacgc
    18361 gctcgctcga acgggcgtcg cgatcgacgg accggtcagc tcggccgatc tgttgcgccg
    18421 gcccggcgtc gaggtcgcca cgctcgcgcc ggcgagcgtc gtgctggccc cgctgacgga
    18481 gcacgaacgc cgcgtcgtcg cggagacgat caagtacgcg ggctacgtcg agcgacagcg
    18541 gcgggaggcg gcccgcgtcg ctcgcgcggg tgcccgacgc attccggccg cgctctgtta
    18601 ccgcgagctg tcgggtctgt cgaacgagct ggtcgaaaag ctcgaggcgg tccgacccga
    18661 gacgctcggc cgcgcgtcgc gcatcgacgg catgacgccg gccgccctct cgctgctcgc
    18721 ggcccacgtc gagcgccacg cggccgttcg gccgtgaccg acggccccga cgaaccatcg
    18781 atttcccgca ccgttgcgcg catcgcggcc gggctcggcc tgtcgctcga cgaggccgca
    18841 cggtcgcgca tcgtccgaca cgcgtcgcgc gttctcggcg ccgggcccga gctgcggctg
    18901 accgcgatct gcgacccgcg agcgttcctc gcgcgacacg tcggcgagag tctcgaaggc
    18961 gcgtcgttga tcgatccggc gacgcgcggc gcgatgctcg atctcggcag cggcaacggc
    19021 taccccggac tgccgatcgc ggcggcgcgc tccgggctga cgccctacct ggccgaagcg
    19081 tcccgaaaga agtcggcgtt cctgcgctcg gtgatcgaag tcgacttccc cggcgcggtc
    19141 gtgctcgacc gccagatcca gcgtcccgcc gacctgcccg ccgacgtccg gttcgcgatc
    19201 atcgtttcgc gggcgatggg caactgggaa cgcgtcgtcc cgcggctcgt cccgtgcctt
    19261 gttcccggcg gccgcgtgct ggtctgggcc ggtccggcga tggagagcgt gtcgcggcgt
    19321 gcgacctgga gccggctgcg accgagcgga cggcgggcac tgaccggacg cgagcgatcg
    19381 tggatctggg ccttcgatac cgttatgtaa ttataattat taaatattgt tccacgtgga
    19441 acaatcggcc gcgtcgctgc gggcatgttc cacgtggaac attccgtctc cgtcttgatt
    19501 ccccgatggt agagtacggc cttcaggggg attctcgtcg aggagtgatc ggtgggccgt
    19561 atcatcgcga tcgtcaacca gaaaggcggt gtcggaaaga cgacgagcgc ggtcaacctc
    19621 gccgccgccc tcgcgattgc cgagcgaccg atcctgctcg tcgacgcgga cccgcaggcg
    19681 aacacgacgc gcgccatcgg tttcgccgca gacccggagc gtccgacgct ctaccaagtg
    19741 ctgatcggcg actttccgat cgaggaaacg acgatccaga gcccggatct cccccacctc
    19801 tcactgctgc cggccgacag cgatctggtc ggcgccgagg tcgaactggt cgaggccgat
    19861 cgccgagagt tccgtctgaa ggagcggatc gagccgctgc ggaatcgctt cgacttcatc
    19921 ttcatcgact gcccgccctc tctcggcctg ctcacgctca acgcgctggt cgccgccgac
    19981 tcggtcatca tcccggtcca gtgcgagtac ctggcgctcg agggcatctc ggcactgacg
    20041 cgcaccatcg agggtgtgcg ggccgcgctg aatccgacgc tcgagatcga cggggtgctg
    20101 atgacgatgt tcgacgagcg gaccaatctc gcgcgccagg tcgtagacga ggtccgtggc
    20161 gtcttcggcg atcgtgtctt cgagaccctg atcccgcgca acgtgcgtct cggtgaggcg
    20221 ccgagctacg gcaagccgat tttcctgtac gacgtgcgct ccaaggggtc cgaggcgtac
    20281 ttcaacctgg ccaaggagtt cgtgaaggat gaagcgaaag gcgttgggca aaggtctgcg
    20341 cagtctgata ccggagccgc cgctgcgcgt ccggaagagc ccgagcgaaa ccgtcccggc
    20401 gagcgacacc ggcctccgcc agatcgacct cgatcggatc tggcctaatc cggcccagcc
    20461 gcgcacgagc ttcgacgagg agcgtctgga cgaactggcc cgctcgatca agaaccaggg
    20521 ggttctccaa ccgatcgtcg tccgtccggg caaggacggc gcctacgagc tgatcgccgg
    20581 agagcgacgt tggcgagcgg cccagatggc cggattgctc aaaatcccgg ccgtcgtgcg
    20641 cgaaacgggt gactcgcagc tccgcgagct cgccctgatc gagaacctcc aacgcgacga
    20701 cctcgatccg atcgaggaag cggcggccta ccaggcgctg atcgacgaag cggatctgac
    20761 gcaacaggac gtcgccgatc gcgtcggcaa gtcccgcgcg accgtcgcga acgcgctccg
    20821 tctgctcaac ctgccggccg aggtccagca gatgatccgc gacggccagc tgagtgcggg
    20881 ccacgccaag gcgctactcg ggctcgcgag ctcgacgggt cagatcgagc tggcccgtcg
    20941 tatcgtgagc gagggtctga ccgtccgctc ggtcgagagc cggcttcgcc ggagcgcgtc
    21001 ggagcgtccg gcaccagcac cggccgaacc ccggcgggat ccgaacgtcg ttgccgccga
    21061 gcagcagttg cagaagacgc tcggcacgaa ggtccgcatc ctgcagcgcc catcgggagc
    21121 aggccggctc gagatccact tcttcagcgg cgaggagctc gaacgcgtct tcgagctcct
    21181 ttccgggact gcgcgggggc ggggttaggc gccgactgtt ttgtcggcag atgttcaaat
    21241 gccgacataa gtgtcggcgc atcacgtctc ccataccgat ccgagccagt ttttcgagag
    21301 atctcgccgg cagacggaat tgtctgcggc accggttggc cgacatttct gtcggcacga
    21361 ggcaaccgag gattcgtctc gtcgtcgacc ggtacctcga ccgcgggtgc ctccagcgta
    21421 cgatcgtcgc cgacacgatt gtcggcggcc gcctctcggc cgacgatcat gtcggcggat
    21481 cctcgacgcc ggccggcgcg gtgcgcaccg acaaacgacc caccccggcg tccatgacgc
    21541 cgacacaatt gtcggcattc tccataggcc gacatttcag tctgcgcttt ggctccgacc
    21601 gacccagcac ggcgctccga tttcttgacc caccaagtac gctgtgataa tttccgccct
    21661 cttcccggag cggaccgagc acgcgggcgg gaaccgcgtt cgactccgtc gggacacggg
    21721 gtcccgggct cttcattaag aggcatcgat gaaagaccgc aagctggcgc tccgctacgc
    21781 gcgcgccctg ctgtccgccc tccacgaccc cgcgatcctc gagaagaccg acgcgttcct
    21841 cgccgccctc gcgcgggcgc tcgagggctc ggccgagttg cgctcggcgc tgctcgaccc
    21901 ggccgtcccg cgcggcgtgc gcaaacgggt cctggcggcg atcgccgagc gcaacgggct
    21961 gccgcgcgag gtccggaact tcctcgccac gctgaccgac aacaaccgca tctcggccct
    22021 ggcgtcgatc gcgaccgtgt tccacgaaga gctggaaaag aagatgggcg tcgtcccggc
    22081 cgagctgatc accgccgcac ccgtcggcga cgagctgctg acgcgcgctc aggcgaccct
    22141 cgagcgggtc accggccgca gagtgcgcct gacctcgcgc gtcgatgcga cgctgatcgg
    22201 cggtgcggtg acccgcgtcg gctcgaccgt ctacgacggc agcctgcgta ggcaaatcga
    22261 ccaactcagg agaaagatgg ccgaagggta ggacggcact cgaccgagga gagacgatgg
    22321 agatcaaggt cgacgagata tcgaagatca tccgccagca gatcgagggt tacgacaagc
    22381 agaccgatct ggccgaggtc ggcacggtga tctcggtcgg ggacggcatc gggcgcatct
    22441 acgggctcga tcgggtgatg gcgggcgagt tgctcgagct gccgcacgac gtgatgggca
    22501 tggcgctgaa cctcgaggaa gacaacgtcg gcgcggtgct gctgggcgag acggtccgca
    22561 tcaaggaagg ggaccaggtc cggcggacca agcgcatcat gcaggtgccg gccggcgacg
    22621 cgatgttggg ccgcgtggtc gatccgctcg gcaagccgct cgacggcaag ggcccgatcg
    22681 cgaccaacgc gtcgatgacg atcgaacgga tcgcccccgg cgtggtccac cgcaaaccgg
    22741 tgaccgagcc gatgcaaacc ggtctcaaga cgatcgacag catgacgccg atcggccgcg
    22801 gccagcgcga gctgatcatc ggcgaccggc agaccggcaa gacggcgatc gccgtcgaca
    22861 cgatcatcaa ccagaagggc caggacgtcg tctgcatcta cgtcgcgatc ggccagaagc
    22921 agtcgacgat cgcgcagttg gtcaagacgc tcgaagagcg cgacgcgatg agctacacga
    22981 tcgtcgtcgc cgcgtcggcc agctcgccgg ccccgttgca gtacctcgcg ccgtacggcg
    23041 gctgcgcgat gggcgagtac ttccagttcc atggagccga cggcaagccg gcgtcgagca
    23101 acaaccccgg gcgccacgtg ctctgcatct acgacgacct atcgaagcat gccgcggcct
    23161 atcgcgagat ctcgttgctg ctgcgccgac cgccgggacg cgaggccttc ccgggcgacg
    23221 tcttctacct ccactcgcgc ttgctcgagc gggcgagcaa gctcaacgcc gacaacggcg
    23281 gcggctcgct gaccgccctg ccgatcatcg agacccaggc cggagacatc tcggcctaca
    23341 tcccgaccaa cgtgatctcg atcaccgacg gtcagatctt cctcgagtcc gacctgttct
    23401 acgccggtgt gcggccggcg atgaacgtcg gcctgtcggt ctcgcgcgtc ggcggcaacg
    23461 cgcaggtcaa ggcgatgaag caggtcgccg gcaccttgcg gttgtcgttg gcccagttcc
    23521 gcgagctcga ggcgttcgcc cagttcggct ccgacctcga caaggcgacg caggcccagc
    23581 tcaaccgcgg ccgccgtctg gtcgagatcc tcaagcaggg ccagtaccag ccgctgccgg
    23641 tggccaagca gatcctggtg atcttcgccg ccgcgagcgg ctatctcgac aacgtgccgg
    23701 tcgaggcgtg ccgcgacttc gagcgcgacc tgtaccagag cttcgacatg aaccaccccg
    23761 gcctgttggc ccagatcgcc gacaagtcgg gtctgaccga cgagcgcaag gaagcgatcg
    23821 tcaaggtggt caaggaggtc gccgaccgtt accgctcggc ggccccggtc gccggctgat
    23881 ccgggtcgga acgcgatggc caagacgcag gatctcaagc gccggatccg atcgatccgg
    23941 aacacgatgc agctcacgcg cgcgatgaag atggtctcgg cggccaagtt gcgtcgcgcc
    24001 caggagcgca tcatgcgctc gcggccctac gtccgtcgca cgcgcgcgat cctggcgagc
    24061 gtcgctgcgc gcgccaaccc cgagctgaac ccgttgctgc aacaacgcga agaccggcgc
    24121 gtcgagctgg tcgtgttgac cggggacaag gggttgtgcg ggtcgttcaa cgccggcatc
    24181 ctgcgccgtg ccgaaggcca cgcccacgag ctggtcggcc gcgatctgcg gttcaccgcg
    24241 atcggcaagc gcggccgcga ccatttccgc cgccggaaca tcggcctggt cgggacctgg
    24301 gccgacgtct tccgcgacgt gcagttcccg ctggcggcgg agatcgccga cggcctgatc
    24361 gagcgctacg tcggccgcga ggtcgacgcg atctacctgg tctacaacga gttcaaatcg
    24421 gcgatccagg cccaaccggt cgtcgtgcgg ctgctgccga tcgagatcac cgatctcggg
    24481 tcgacggacg gcggcgaaga ctacatctac gaaccggagc cgcagcagct gctggccgcg
    24541 ctgctgccgc actacgtcca gcagctcgtg ttccaggcgc tgctcgaatc cgtcgccgcc
    24601 gagcacgcgg cgcggatgac cgcgatggac agcgccacga agaacgccgg ggagctggtc
    24661 gactcgctga ccctgaccat gaaccgcgta cgccaggcct cgatcacgac cgagatcatc
    24721 gaggtcgtct cgggcgcgca aggacaagga tgaacggacg gagcagtcga tgagcaagat
    24781 cgggaaggtc gtacagatca tcgggcccgt gctcgacatc gagttcgacg acgagcactt
    24841 gccggcgatc tacaacgcgg tgcggatccg agacgacggg agcgaatcgg ggacgaaaat
    24901 cgacatcatc gccgaagtcg agcagcacct gggcgagaac cgagtgcgct gcgtggcgat
    24961 ggaaccgacc gacggcatgg tgcgcggcat gagcgccgag gacctgggcg aaccgatcaa
    25021 ggtcccggtc ggcgaggcca ccttgggtcg tgtgctgaac gtgatcggca acccggtcga
    25081 cgagctcggt ccggtcgagg ccgaggacca ctggccgatc caccgcgaag ccccggcctt
    25141 cgacgagcag gacacttcga tcgagatgtt cgagaccggg atcaaggtga tcgacctgct
    25201 cgagccgtac ctcaagggcg gcaagaccgg cctgttcggc ggcgcgggcg tcggcaagac
    25261 ggtgttgatc atggagctga tccacaacgt ggcgctcaag cacggcggct tttcggtttt
    25321 cggcggcgtc ggcgagcgca cccgcgaggg caacgatctg tggctcgagt tccaggagac
    25381 cggcgtcatc aatatcgccg agccctccaa gtcgaaggcg gcgctgatct acggccagat
    25441 gaccgagccg ccgggggccc gtctacgcgt ggccctgtcg gcgctgaccg tcgcggagta
    25501 cttccgcgac gtcaagcacc aggacgtgct gctgttcatc gacaacatct tccgcttcac
    25561 ccaggccggt tcggaggtct cggcgctgct cggccgcatg ccgtcggccg tcggctacca
    25621 gccgacgctc gccagcgaaa tgggcgagct gcaggagcgc atcacctcga ccaagaccgg
    25681 ttcgatcact tcggtgcagg cgatctacgt gccggcggac gactataccg acccggcgcc
    25741 ggcgacgacc ttcgcccacc tcgacgcatc gaccaacctg tcacggcaga tcgccaacct
    25801 cggcatctac cccgcggtcg atccgctcgc atcgacctcg cgcatcctcg acccgcagat
    25861 cctgggcgag aaccactacg acacggcacg cgcggtccag caggtgctgc agcgccaccg
    25921 cgagctgcag gacatcatcg cgatcctcgg catggacgag ctgtccgacg acgacaagct
    25981 gacggtggcc cgggcgcgca agatccagcg attcttctcg cagccgtgcc acgtcgccga
    26041 gcagttcacc gggatgcccg gccgctacgt caaggtcgag gacacgatcc gcgggttccg
    26101 cgagatcgtc gacggcaagc acgatcaact gccggagcag gcgttcctct acgtcggtac
    26161 gatcgaagag gcgctggaga aggcggagaa gatccgtcgc gagaccgccg cgtaaccggg
    26221 gctttcgatg gccgacaagc tcaagctcga agtcgtgacc cccgatcact cggtgctgga
    26281 tcaggaggtc gacgaggtgg tgttgccgag cgtcgagggg tacttcggcg tgctacccgg
    26341 ccacgcgccg ttgctcgcgc aactcgacgt gggcgaggta tcctatcgtg tcggaaagga
    26401 acggcggttc ctggcgatct cgggtggtta cgccgaggtg ctgcgcacgg gcgtcagcgt
    26461 gctcgcgcga acggccgagc gggcggacga gatcgacgtc gaccgggccc ggagagccaa
    26521 ggatcgcgcc gagtcgcagc tgaagcagaa cctcgcgctc gacgacctcc gccgcgccga
    26581 agtcaagctg aagcgggcga tcagccggct atcggttcac gatcgaagct gaggaggacg
    26641 gcgatgggcg
//
LOCUS       DASPGN010000160         5859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_612830, whole genome shotgun sequence.
ACCESSION   DASPGN010000160 DASPGN010000000
VERSION     DASPGN010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612830"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..277)
                     /locus_tag="VD788_04545"
     CDS             complement(116..277)
                     /locus_tag="VD788_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75566.1"
                     /translation="MIAEAKSVTVVEELESDPPGVGVTVGIDFNGERVELGSFVARLI
                     RGTMSGLRR"
     gene            370..1149
                     /locus_tag="VD788_04550"
     CDS             370..1149
                     /locus_tag="VD788_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75567.1"
                     /translation="MGSYELRPLAIFPALLLTAACTSGVESVVAKDATRARAIELLVG
                     DAAMSGEVIEGLLGNPDARVALSAKIADDEAFAGALVDRVMQSDRGKAVVASRIGTDS
                     ATTRTLLGMLMATGAMGRIISQQQAECFDLGEQFALGNQRRTMVDLKRLGSVVDRWAR
                     EHGGAYPVCDPFSDVAGCLASSLPAGAFAELRLEDAWGRPFVYHSDPGGSSYALISYA
                     TDGRYDELGRVGPTSSYDADIVYSEGDFVQWPGTILKEQVR"
     gene            1248..1778
                     /locus_tag="VD788_04555"
     CDS             1248..1778
                     /locus_tag="VD788_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75568.1"
                     /translation="MNAHCRFVLALVLASLAAACSGSRSTGSAAGGTASLSVDGTTYH
                     VHDVSMTIELGEQAWFRIDGEPVGDPHADCVPGLGAGLGLYGDLPDSVRQPIDLGGKR
                     LRVDFTGDGDDANFCFVGMGGLAGAEDAWVTIESVNGDRVAFSMSGTFRVYDENGDGP
                     VRTAQASGTALLAGAS"
     gene            2115..2402
                     /locus_tag="VD788_04560"
     CDS             2115..2402
                     /locus_tag="VD788_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXV75569.1"
                     /translation="MKVKPLYDRVLIRRIEQKEQRKGGIIVPDTAKEKPMEGKVVAVG
                     SGKVDKDGNVIPLQVKAGDRVLVGKYAGTEIKIDDEEHVIVREDEVLGIIS"
     gene            2444..4078
                     /gene="groL"
                     /locus_tag="VD788_04565"
     CDS             2444..4078
                     /gene="groL"
                     /locus_tag="VD788_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXV75570.1"
                     /translation="MAAKSITYADQGRQAMLRGVNRLANAVKVTLGPKGRNVVLEKKY
                     GSPLSTKDGVTVAKEINLADPAENMGAQLVREVASKTSDVAGDGTTTATVLAQAVFRE
                     GVKAVTVGGNPMDIKRGIDAATEAAVAAIKKLSRPVAGKAIAQVGSISANNDPTIGKI
                     IAEAMDKVGKDGVITVEEAKGMETSLEVVEGMQFDRGYLSPYFVTDTERMQCELEDPY
                     ILIHDKKVSSMNDLLPILEQVAKAGKPFVIVAEDVEGEALATLVVNKLRGTLQAAAVK
                     APGFGDRRKAMLQDLAILSGGTCITEDLGIKLENIKLADLGRAKKIVIDKDNTTVIEG
                     AGKAKDISGRVQQIRTQIEESTSDYDREKLQERLAKLVGGVAVIKVGAATESEMKEKK
                     ARVEDAMHATKAAVEEGIVPGGGVALLRSVKALDAVAKSEKNEDRATGVRIVRRAVEE
                     PLRQIAANAGADGSVVVNQVRELEPDKGFNAQDESYVDMIEAGIIDPTKVVRIALQNA
                     ASIAGLMLTTEALIAEVPEKEKPAAPGAGAGMGEDF"
     gene            4215..4694
                     /locus_tag="VD788_04570"
     CDS             4215..4694
                     /locus_tag="VD788_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXV75571.1"
                     /translation="MERREEIARAVLTIIGARGRTALTTTTLAAEVGLTTGALYRHFA
                     SLDEILTEAVRYGVERIESTFPAAALPPLDRVLELARNRVRLLGRDRGLAWLLRSEQA
                     YLTLPIHAVIGVGHGDHALSGEAPPDPERVLDTLARLLAPPVHSGRSKRESTKGEPE"
     gene            4691..5350
                     /gene="ric"
                     /locus_tag="VD788_04575"
     CDS             4691..5350
                     /gene="ric"
                     /locus_tag="VD788_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HXV75572.1"
                     /translation="MNIHPETTVGRIATEHPLATRVFARHGIDFCCGGGKPLAEVCQR
                     RGLDVASVVAEIERELAEPDSAEGRWDQAPLADLIDHIVSSYHRPLKTELPRLEALAR
                     KVVDVHRDKRPELLSVFLGLKTELEQHMGKEELILFPMIKRGQGALAGGPVSVMEHEH
                     DVAGNALERMRELTDDFRVPAEACNSWRALWHGLAALEREMHQHIHLENNILFPRALA
                     S"
     gene            5545..>5859
                     /locus_tag="VD788_04580"
     CDS             5545..>5859
                     /locus_tag="VD788_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75573.1"
                     /translation="MTDQPTTRLDRRDAVLEAAIRLGVSALLLYWALRILSPFFHVVI
                     WSSIIAVSVYPLFVRLRRLVGGRNRLAGGLFLLLALALLVLPVVGLALESIERAREFA
                     THW"
BASE COUNT         1023 a         1892 c         2055 g          889 t
ORIGIN      
        1 cccccccccc cgccgccgca ggcaatactc ggacaggctc ctagcgttcg ccgacgccgt
       61 gcttcacccg cgctcggcgg tccgggcgac ggactcgcgg tgcatcaggc cctcctcagc
      121 gacgaagccc ggacatggta ccccggatca ggcgggcgac gaacgaaccg agctcgacgc
      181 gttctccgtt gaagtcgatt cccaccgtca cgccgactcc cggcggatcg gactcgagct
      241 cctcgaccac cgtgacggac ttcgcttctg caatcacgag cacattggtg aaagccgcgc
      301 attcgtcctt gatccgggcc cggcgccggc tagattcgac cgtgtgggac gctggatcag
      361 gaggccgtca tgggctctta cgagctacga ccgttggcga tattccccgc gttgctgctg
      421 accgccgcat gcacgagcgg cgtcgagtcg gtcgtggcga aggacgcgac gcgcgcgcgg
      481 gcgatcgagc tgctcgtcgg cgatgcggcg atgagcggcg aggtgatcga gggtctgctg
      541 ggcaaccccg acgcacgagt cgcgctatcg gcgaagatcg cggacgacga ggcattcgcc
      601 ggagcgctcg tcgatcgagt gatgcagagc gatcgcggca aggccgtcgt cgcgagccgc
      661 atcggcacgg actccgcgac gacccgcacg ttgctcggca tgctgatggc gaccggcgcg
      721 atgggccgga tcatcagcca gcagcaggcg gagtgcttcg acctcggcga gcagttcgca
      781 ctcggaaacc agcgccggac gatggtcgat ctgaagcggc tcggcagcgt cgtcgaccgc
      841 tgggcgcgcg agcacggtgg agcgtacccc gtctgcgacc cgttcagcga cgtcgccggc
      901 tgcctcgcat cgagcctgcc ggccggggca ttcgccgagc ttcggctgga ggacgcctgg
      961 ggccggccgt tcgtctacca cagcgacccg ggcggcagca gctacgcgct gatctcctac
     1021 gccaccgacg ggcgatacga cgagttggga cgggtcggtc cgacctcgtc ctacgacgcc
     1081 gacatcgtct actcggaagg ggatttcgtg cagtggccgg ggacgattct caaggaacaa
     1141 gtccgctgag aggctcctgg cggccaagag gggcggttcg ctggacaaga ccgcggccgg
     1201 ctccgatatt gcccgggtcg gcggcccagc ccgcacaggg aggtccgatg aacgctcact
     1261 gccgtttcgt gctcgcactg gttctcgcgt cgcttgccgc cgcctgctcg ggatcgcgct
     1321 cgacggggtc cgcggcgggt ggtaccgcca gtctgtcggt cgacggtacg acgtaccacg
     1381 tacacgacgt atcgatgacg atcgagctcg gggagcaagc gtggttccgc atcgacggcg
     1441 aacccgtcgg cgacccgcat gcggactgcg tgccgggatt gggcgccggt ctgggcctct
     1501 acggcgatct tcccgattcg gtgcgacagc cgatcgacct cggcgggaag cgcctgcggg
     1561 tcgacttcac cggcgacggg gacgacgcca acttctgctt cgtcggcatg ggggggctcg
     1621 ccggggccga ggatgcatgg gtcacgatcg agtcggtgaa cggcgatcgc gtcgcgttct
     1681 cgatgagcgg caccttccgt gtctacgacg agaacggcga cggtcccgtg cggaccgccc
     1741 aggcctcggg gaccgcgctc ctcgccggtg cgtcgtgacc ggtagcgcct agccgttcgg
     1801 gccgggtaga ggcccggtcc aggccttttt ctacgacggc ggaccttgac agggcccctc
     1861 gccgattcca gtcccatgcc gggcggcgcg ggagaagctg aacgcgccgc gcgcgcccgg
     1921 cgctccggca cgcgcccctg acctcgcaaa ctgttgacag aaggagggtt cggcacggtc
     1981 gcccgcagcg tccggcccct ccgggtggat cgcgacggtc cggggacctc gtggctcagg
     2041 gcgcaccggc gtgtcgccag acgagcacgg tcgatttcac gacgtcggac gacgtcgagt
     2101 ggagtgagga aaccatgaag gtgaagccgt tgtatgaccg ggtcctgatt cgtcggatcg
     2161 agcagaagga gcaacgcaag ggcgggatca tcgtccccga cacggcgaag gagaagccga
     2221 tggagggcaa ggtggtcgcc gtcggtagcg gcaaggtcga caaggacggc aacgtcatcc
     2281 cgctgcaggt caaggccggc gatcgcgtgc tggtcggcaa gtacgccggg accgagatca
     2341 agatcgacga cgaagagcac gtgatcgtcc gcgaggacga agtgctcggc atcatcagct
     2401 gacaatcgac tatcgaaccg gcgacggttc cggaggacaa cgtatggctg cgaaatcgat
     2461 tacctacgcc gaccagggtc gccaggcgat gctgcgcggc gtcaaccgat tggcgaacgc
     2521 ggtgaaggtc actctcggcc ccaaggggcg caacgtcgtg ctcgaaaaga agtacggttc
     2581 cccgctgtcg acgaaggacg gggtgacggt cgccaaggag atcaacctgg ccgatcccgc
     2641 tgagaacatg ggcgcccaac tcgtgcgcga ggtggcgagc aagacctcgg acgtcgccgg
     2701 ggacggcacg acgacggcca ccgtgctggc gcaggcggtc tttcgcgagg gcgtgaaggc
     2761 cgtcaccgtc gggggcaatc cgatggacat caagcgcggg atcgacgccg cgacggaagc
     2821 ggccgtcgcc gcgatcaaga agctctcgcg cccggtcgcg ggcaaggcga tcgcccaggt
     2881 cggttcgatc tcggccaaca acgacccgac gatcggcaag atcatcgccg aggcgatgga
     2941 caaggtcggc aaagacggcg tgatcaccgt cgaggaagcc aagggcatgg agacctcgct
     3001 cgaggtggtc gaaggcatgc agttcgaccg tggctatctc tcgccctact tcgtcaccga
     3061 cacggagcgg atgcagtgcg agttggagga tccctacatc ctgatccacg acaagaaggt
     3121 cagctcgatg aacgacctgc tgccgatcct cgagcaggtg gccaaggccg gcaagccgtt
     3181 cgtcatcgtc gccgaggacg tcgagggtga ggcgttggcg acgctcgtcg tgaacaagct
     3241 gcgcggcacg ctccaggcgg cggcggtcaa ggcgccgggc ttcggcgatc gccgcaaggc
     3301 gatgttgcag gatctggcga tcctcagcgg cggcacctgc atcaccgagg atctcgggat
     3361 caagctcgag aacatcaagc tcgccgacct gggacgcgcc aagaagatcg tgatcgacaa
     3421 ggacaacacg accgttatcg agggcgccgg caaggcgaag gatatctccg ggcgcgtcca
     3481 gcagatccgc acccagatcg aggagtcgac gtcggactac gaccgagaga agctgcagga
     3541 acggctggcc aagctggtcg gcggtgtcgc ggtgatcaag gtcggcgcgg cgaccgagag
     3601 cgagatgaag gagaagaagg cgcgcgtcga ggacgcgatg cacgcgacca aggcggccgt
     3661 cgaagagggg atcgtcccgg gcggtggtgt cgcgctgctg cggtcggtca aggcgctcga
     3721 cgcggtggcc aagagcgaga agaacgagga tcgggcgacg ggcgtgcgca tcgtccggcg
     3781 cgcggtcgag gagccgttgc ggcagatcgc ggccaacgcc ggggccgacg gctcggtcgt
     3841 cgtcaaccag gtccgcgagc tcgagcctga caaggggttc aacgcacagg acgagagcta
     3901 cgtcgacatg atcgaggccg ggatcatcga cccgaccaag gtcgtccgga tcgcgctgca
     3961 gaacgcggcg tcgatcgccg gcttgatgct cacgaccgag gcgctgatcg ccgaggtacc
     4021 ggaaaaggag aagcccgcgg cccccggtgc cggcgccggc atgggggagg acttctgagc
     4081 cggccggtct gaagagtcgg acgaacgggg gagagggtgc gcctggcgca ctttctcccc
     4141 tttttgggag attgacagta agtaaatagt gacttacaat atcgaccgtg cgcccgaccg
     4201 caagaaagca gaccgtggaa cgccgggaag agatcgcccg cgcggtgttg accatcatcg
     4261 gcgcgcgggg ccggactgcg ctgaccacga cgacgctcgc cgcggaggtc ggcctgacca
     4321 ccggtgcgct ctaccgccac ttcgcttcgc tcgacgagat cttgaccgag gccgtacgct
     4381 acggcgtcga gcggatcgag tcgaccttcc ccgccgccgc gttgccgccg ctcgaccgcg
     4441 tactcgagct cgcgcggaat cgggttcgcc tgctcgggcg ggaccgcggt ttggcctggc
     4501 tgttgcgctc cgagcaggcc tatctgacgc ttccgatcca tgccgtgatc ggggtcggtc
     4561 acggagacca cgccctgtcc ggggaggccc cgcccgaccc ggagcgtgtg ctcgacacgc
     4621 tcgcgcgcct gctcgccccg cccgttcact cgggtcgatc gaaacgagaa tcgacgaaag
     4681 gagaaccgga atgaacatcc acccagaaac caccgtcgga cggatcgcca ccgagcaccc
     4741 gctcgcaacg cgggtcttcg cccggcacgg catcgatttc tgctgcggcg gcggcaaacc
     4801 gctggccgag gtctgccagc gacgcggtct cgacgtcgcg tccgtcgtcg ctgagatcga
     4861 acgggagctg gccgagcccg attcggccga gggccgctgg gatcaggctc cgctcgccga
     4921 tctgatcgac cacatcgtct cgtcctacca ccgaccgttg aagaccgagc tgcctcggct
     4981 cgaggcactg gcgcgcaagg tcgtcgacgt ccaccgggac aagcgtcccg agctgctgtc
     5041 ggtatttcta ggcctcaaga ccgagctcga gcaacacatg ggcaaggaag agctgatcct
     5101 gttcccgatg atcaagcgcg ggcagggggc gctagccggc ggaccggtgt cggtgatgga
     5161 acacgagcac gacgtcgccg gcaacgcgct cgagcgaatg cgcgagctga ccgacgattt
     5221 ccgcgtcccc gccgaggcgt gcaacagctg gcgagcgttg tggcacggac tggccgcact
     5281 cgagcgggag atgcatcagc acatccacct cgagaacaac atcctctttc cgcgcgcgct
     5341 ggcgagctga cggggactgg accgccgact ggctgcatcg cgaggcctcc aggcccgcct
     5401 cgagcgggat cttcggtttc ctgatcaacc cgccgatcgc gcccagcggc tggcaagcgt
     5461 cgaccacgga ctgtagtacg cacggtccgg gtggtcagtg gcccctgatg caagcggacc
     5521 acacagggtt tatcatcggc gctcatgacg gaccaaccga cgacgaggct cgaccggcgg
     5581 gacgcggtgc tcgaggccgc gatccgtctc ggtgtttcgg cgctgctcct gtactgggcg
     5641 ctgcggatcc tctcgccgtt cttccacgtg gtgatctgga gctcgatcat cgccgtgtcg
     5701 gtctatccgt tgttcgtgag actgcgacgt ctcgtgggtg gacggaaccg gctcgccggc
     5761 gggctgttcc tcctgctcgc gctcgccctg ctggtcctgc cggtcgtcgg gctggcgttg
     5821 gagtcgatcg aacgggctcg ggagttcgcc acccattgg
//
LOCUS       DASPGN010000161         6049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1666339, whole genome shotgun sequence.
ACCESSION   DASPGN010000161 DASPGN010000000
VERSION     DASPGN010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666339"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VD788_04585"
     CDS             <1..926
                     /locus_tag="VD788_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HXV75574.1"
                     /translation="FEREPDGTPTTRTHDRAAYFDLAPIDRGEQCRREIVNRLEALGF
                     EVEAAHHEVAPGQHEVDFKYDEAVRTADKIATFKLVVRHVATQHDLHATFMPKPLDGV
                     NGSGMHTHQSLFRDGVNAFEDRRAPLGLSQTALYYVGGLLAHAKGICAVTNPLVNSYK
                     RLVSGYEAPTNVVWSERNRSPLVRVPERRGSGTRVELRSPDPSCNPYLAFAVMIAAGL
                     DGVVNELDPGPPINKNIFTMSHREKRRLRIDELPSDLKEAVRWLKKDPLPRQALGDHV
                     YNHFVEAKLASWRDYSSKVHPWELERYLARY"
     gene            926..1708
                     /locus_tag="VD788_04590"
     CDS             926..1708
                     /locus_tag="VD788_04590"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXV75575.1"
                     /translation="MGATTPRRRVPSDADAWSRLERAIVSCTRCKRLIAHCREVARRR
                     RRAYRDEIYWGRPVTGFGDLDARILMLGLAPGAHGANRTGRIFTGDRSGDFLYGALHR
                     AGLANQSRAVGVDDGLRLRRVFVSAACRCAPPGNRPSPAELRNCAGYLARELDLLARL
                     RVVVALGRIAWEAALRRAREIAAGSPFRAPRFGHGAEARLTLATGGLPLWLVGSYHPS
                     QQNTQTGRLTRAMFDRVIRRAVALARSDEYPGSRHETAPPVQ"
     gene            1722..3134
                     /gene="glnA"
                     /locus_tag="VD788_04595"
     CDS             1722..3134
                     /gene="glnA"
                     /locus_tag="VD788_04595"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HXV75576.1"
                     /translation="MTPKQFFALAKKHGAEMIDLKFTDLLGTWQHCSFPTDSWSEATF
                     KEGVGFDGSSIRGWQGIHVSDMLAVPDPTTAVLDPFFAEPTISVIADIKDPVTLEDYS
                     RDPRHVARKATDYLKKTRIADTCYVGPEPEFFIFDEVRYEQTQHRGFYQIDSVEGAWN
                     TARFEEPNLGYKPSYKGGYFPVSPSDTYHDLRGEMVKIMKQVGLVVEAHHHEVGTAGQ
                     SEIDVRFEPLQQMADHFMWFKYVIKNVAKRHGKTVTFMPKPIFEDNGSGMHTHVSLWK
                     AGRPLFAGKKYAGLSEIALHAVGGLLKHAKAVLAFAAPTSNSYRRLVPGFEAPVNLVM
                     SARNRSAAVRIPMYSSSPKAKRVEFRCPDPSCNGYLTFSAILMAMIDGVKNRIDPGEP
                     LDRDIYEMTPDELAGSPKTPARLEEALEALAADHEFLLAGGVFTDDLIETWIEYKMEN
                     EVDPLRLRPHPYEFHLYYDN"
     gene            complement(3175..3369)
                     /locus_tag="VD788_04600"
     CDS             complement(3175..3369)
                     /locus_tag="VD788_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75577.1"
                     /translation="MKKWIERAGNLSGVVGLAVCVVAGLSRLTGAFYTAGFQTMVLFN
                     VGVGLMVAGCLAKLHALSER"
     gene            complement(3387..4100)
                     /locus_tag="VD788_04605"
     CDS             complement(3387..4100)
                     /locus_tag="VD788_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXV75578.1"
                     /translation="MQPLLVLFDIDGTLVDTAGAGRRSIERAFVELWRVDPTTAAGRR
                     VPYAGRTDPQIFRALARAFAVDPDRYRAGEAALVELYLAALVEEMARPDPRRRVLPGV
                     AALLEGLCGTPGVHLGLVTGNLERGARAKLAPFGLSDYFPAGGFGSDHVERASIARVA
                     HHRLSRHAGIDFPPSRVVVVGDTEHDVACARANGFRALAVATGTIPSETLRRAGPDAL
                     FSTFDERPDAVRAALGLPS"
     gene            4161..5246
                     /locus_tag="VD788_04610"
     CDS             4161..5246
                     /locus_tag="VD788_04610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXV75579.1"
                     /translation="MRVESRLVQVALRGHEVRTPGEPIEGVERLLVVRNDRLGDLLLT
                     LPAIDALARTYPGARVGLLVGRELSDIGGMIRPVERLFAVERRAQAVRGAIEAFAPQL
                     VVAISRRPAPAWAAARAGVPHRVGTGYRYYSPLFTRRVDERRRSSTRHEVEYALSFAH
                     RAGARPAEPDFSLRVPGEARERVAAWLSGRGLADRFAIVHPGTGGSCPAWPLPHYRTL
                     VASLAALGHAVVVTVGPDDREIVDGWERAGAEGSVACFRQSTPLLAALVERAALVVSN
                     STAPIHLAAALGRPALALHAPWPSCGPDRWGPYHRAGWALVAESTGAAGWSRRERECK
                     ATVLMAGIAPERVLQAVVSMLGTGRPV"
     gene            complement(5253..5876)
                     /locus_tag="VD788_04615"
     CDS             complement(5253..5876)
                     /locus_tag="VD788_04615"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HXV75580.1"
                     /translation="MEQRHATTGRRIVVAVTGASGAIYAVRFVKACLELGARVELVAS
                     EYGRRLLIDECELNLKSTTIEQWLDDRYGAGARTGSVRLHRSNDLGDEIASGSQRWDA
                     MVIVPCSMKTLSGVAHGSATNLIERAADVTLKEARPLVIVPRECPLNLIQLDNLTRAA
                     RAGAKIVPAMPAFYFKPRDFDDLADFIAGRILALLGIEHRLFEPWNG"
BASE COUNT          956 a         2160 c         2044 g          889 t
ORIGIN      
        1 tgttcgagcg cgagcccgac gggacgccga ccacccggac ccacgaccgc gcggcctact
       61 tcgatctggc gccgatcgat cgcggcgagc agtgccggcg cgagatcgtc aatcgtctcg
      121 aagcactcgg gttcgaggtc gaggccgcgc accacgaggt cgctcccggc cagcacgagg
      181 tggacttcaa gtacgacgag gcggtccgca cggcggacaa gatcgccacg ttcaagctcg
      241 tcgtgcgtca cgtcgcgacg cagcacgacc tgcacgcgac gttcatgccc aagccgctcg
      301 acggcgtcaa cggctccggc atgcacacgc accagagcct gttccgcgac ggcgtcaacg
      361 cgttcgagga ccgacgtgca ccgctcggac tgtcgcagac ggcgttgtac tacgtcggcg
      421 gtctgctggc gcacgccaag ggcatctgcg ccgtgaccaa cccgctcgtc aactcctaca
      481 aacgcttggt ctcgggttac gaagcgccga ccaacgtcgt gtggtccgaa cgcaaccgct
      541 cgccgctcgt gcgggttccc gagcggcggg gctccgggac gcgggtcgag ctgcgctcgc
      601 cggatccgtc ctgcaatccg tacctcgcct tcgccgtgat gatcgccgcc gggctcgacg
      661 gagtggtcaa cgagctcgac cccggcccgc cgatcaacaa gaacatcttc acgatgtcgc
      721 accgcgaaaa gcggcgcttg cgcatcgacg agctgccgtc cgacctcaag gaggccgtgc
      781 gctggctcaa gaaagacccg ctgccgcgac aggcactggg cgaccacgtc tacaaccact
      841 tcgtcgaagc gaaactggcc tcctggcgcg actacagctc gaaggtccat ccgtgggagc
      901 tcgaacgcta tctggcgcgt tactgatggg cgcgaccacg cctcggcggc gcgttccgtc
      961 cgacgcggac gcctggagcc gtctcgagcg ggctatcgtc tcctgcacgc gatgcaaacg
     1021 gctgatcgcg cattgccggg aggtcgcccg gcggcgccgc cgagcctatc gcgacgagat
     1081 ctactggggg cgtccggtga cgggcttcgg tgacctcgac gcgcggatcc tgatgctcgg
     1141 cctggctccc ggcgcacacg gcgcgaaccg gacgggtcgc atcttcaccg gtgaccgatc
     1201 cggcgatttc ctctacggcg cgctccatcg cgcgggcctg gccaaccagt cccgcgccgt
     1261 cggcgtcgac gacggcctgc ggttgcggcg ggtcttcgtc agcgcggcgt gccgctgtgc
     1321 cccgcccggc aaccggccgt cgcccgcgga gctgcgcaat tgcgcgggct acctcgcgcg
     1381 cgagctcgac ctgctcgccc ggctgcgggt cgtcgtcgcg ctcggtcgga tcgcctggga
     1441 ggcggcgctc cgccgcgccc gcgagatcgc cgcgggctcc ccgttccggg caccgcgctt
     1501 cggtcacggc gccgaggccc gcttgacgtt ggcaaccggc gggctcccgc tgtggttggt
     1561 cggcagctac cacccgagcc aacagaacac ccagaccggc cggctgaccc gcgcgatgtt
     1621 cgaccgcgtg atccgccgcg cggtcgcgct cgctcgaagc gacgagtacc ccggatcccg
     1681 tcacgaaacg gcaccacccg tgcaatagga gtaacgaacc gatgacgcca aaacagttct
     1741 tcgcactggc caagaaacat ggcgcggaga tgatcgatct gaagttcacc gacctgctgg
     1801 ggacctggca gcactgctcg ttccccaccg atagctggag cgaggcgacg ttcaaggagg
     1861 gtgtcggttt cgacggctcg tccattcgcg gctggcaagg gatccacgtc tcggacatgc
     1921 tggccgtgcc cgacccgacg accgcggtgc tcgacccgtt cttcgccgag ccgacgatca
     1981 gcgtcatcgc cgacatcaag gacccggtga cgctcgagga ctactcgcgc gacccacgcc
     2041 acgtggcgcg caaggcgacc gattatctca agaagacccg catcgccgac acctgctacg
     2101 tcggacccga gcccgagttc ttcatcttcg acgaggtgcg ctacgagcag acgcagcacc
     2161 gcggtttcta ccagatcgat tcggtcgaag gcgcgtggaa cacggcgcgc ttcgaagagc
     2221 cgaatctcgg ctacaagccg agctacaagg gcggctattt tccggtcagc ccgtccgaca
     2281 cttaccacga cctgcgcggc gagatggtca agatcatgaa gcaggtcggg ctggtcgtcg
     2341 aggcccacca ccacgaggtc ggcaccgcgg gtcagagcga gatcgacgtc cgcttcgagc
     2401 cgttgcagca gatggccgac cacttcatgt ggttcaagta cgtgatcaag aacgtggcca
     2461 agcgtcacgg caagacggtc acgttcatgc cgaagccgat tttcgaggac aacggcagcg
     2521 gcatgcatac gcacgtgtcg ctgtggaagg cgggtcgacc gttgttcgcg ggcaagaaat
     2581 acgccgggct cagcgagatc gcgctgcacg cggtcggcgg gctgctcaaa cacgccaaag
     2641 cggtgctggc cttcgccgcg ccgacgagca attcctaccg gcggctggtt ccggggttcg
     2701 aggcgccggt caatctcgtg atgtcggcgc gcaaccgctc ggcggcggtg cggatcccga
     2761 tgtactcgag cagtccgaaa gccaagcgcg tcgagttccg ctgccccgat ccgagctgca
     2821 acggctacct cacgttctcc gcgatcctga tggcgatgat cgacggcgtc aagaaccgga
     2881 tcgatcccgg cgagccgctc gaccgggaca tctacgagat gaccccggac gagctggccg
     2941 gctcgcccaa gacgccggca cgtctcgagg aggcgctcga ggcgctggcc gccgaccacg
     3001 agtttctgct ggcgggcggc gttttcaccg acgacctgat cgaaacgtgg atcgagtaca
     3061 agatggaaaa cgaagtcgat ccgctgcggc tacgccccca tccgtacgag ttccatctgt
     3121 actacgacaa ctgatcggcg acggacgggg acgggtgctc gcgacccgcc cccgctacct
     3181 ctccgacagc gcgtggagct tggcgagaca gcccgccacc atcaggccga cgccgacgtt
     3241 gaacagcacc atcgtctgga acccggcggt atagaacgca ccggtcagcc tggacagacc
     3301 ggcgacgacg caaacggcca ggcccacgac cccactgaga ttcccggccc gctcgatcca
     3361 cttcttcatc cgcgccctcc tcgccatcac gacggcagcc cgagcgcggc ccgaaccgcg
     3421 tccggccgct cgtcgaacgt cgagaatagc gcgtccggtc ccgcgcgccg aagggtttcc
     3481 gacggaatcg tgccggtggc cacggcgagg gcgcggaagc cgttcgcccg cgcgcacgcc
     3541 acgtcgtgct cggtgtcccc gacgaccacg accctcgatg gcgggaaatc gatgccggcg
     3601 tggcgggaga gccggtgatg ggcgacccgg gcgatgctcg cgcgttcgac gtggtccgag
     3661 ccgaagccac cggccgggaa gtagtccgac aatccgaacg gcgcgagctt cgcccgcgcg
     3721 ccccgttcga ggttgccggt gaccagcccg aggtgcacgc ccggcgtgcc gcacagcccc
     3781 tcgagcagcg ccgcgacacc cggcaggacc cgccggcggg ggtcggggcg cgccatctcc
     3841 tcgacgagcg ccgcgaggta gagctcgacg agcgccgcct cgccggcccg atagcgatcg
     3901 ggatcgacgg cgaacgcgcg ggccagggcg cgaaagatct gcgggtcggt cctcccggcg
     3961 tagggcactc gccggccggc ggccgtcgtc ggatcgacgc gccacagctc gacgaacgcg
     4021 cgctcgatcg aacgccggcc cgcccccgcc gtgtcgacca gggtcccgtc gatgtcgaac
     4081 aagaccagca gcggttgcat ccggtcattc tagcgatacc ggtgctccgg aatcgagcga
     4141 tccggcggac aatggagacc atgcgggtcg agtctcggct ggttcaggtc gcgttgcgcg
     4201 ggcacgaggt ccggacgccg ggggagccga tcgagggtgt cgaacggctg ctcgtcgtgc
     4261 gcaacgaccg gctcggcgac ctgctgctga cgctccccgc gatcgacgcc ctggcccgca
     4321 cctaccccgg cgcccgcgtc gggttgctcg tcggtcgcga gctgagcgac atcggcggga
     4381 tgattcggcc ggtcgagcgc ctgttcgccg tcgagcgccg tgcgcaggcc gtgcgcggag
     4441 cgatcgaggc cttcgcgccg cagctcgtgg tagcgatctc gcgccggccg gcgcccgcgt
     4501 gggccgccgc gcgggccggc gtgccccacc gcgtcggtac cggctaccgc tactactcgc
     4561 cgttgttcac gcgacgggtc gacgagcgcc gacggtcgtc gacgcggcac gaggtcgagt
     4621 acgcgttgtc gttcgcccat cgcgccggcg cgcggccggc cgagcccgac ttctcgttac
     4681 gcgtcccggg tgaggcgcgc gagcgggtgg cggcctggct ctccgggcgt ggcctggccg
     4741 accggttcgc gatcgtgcat cccggaacgg gtggttcctg cccggcctgg cccttgcctc
     4801 actaccggac gctggtcgcg tcgctcgccg cgctcggcca cgccgtggtc gtcaccgtcg
     4861 ggcccgacga ccgcgagatc gtcgacggct gggagcgggc gggagccgag ggttcggtcg
     4921 cgtgcttccg gcaatcgacg ccgctgctcg ccgcgctcgt cgagcgtgcc gcgctcgtcg
     4981 tcagcaacag caccgcgccg atccacctgg cggccgcgct cgggcggccg gcgctcgccc
     5041 tgcacgcccc gtggccgagc tgcgggccgg atcgctgggg cccgtaccac cgcgccggtt
     5101 gggcgctcgt cgccgaatcg accggtgcgg ccggctggtc gcgacgggaa cgcgagtgca
     5161 aggcgacggt gctgatggcc ggcatcgcgc ccgagcgcgt ccttcaggcg gtcgtctcga
     5221 tgctgggtac ggggcggccg gtctgaaccc cgtcacccgt tccacggttc gaacaggcgg
     5281 tgctcgatcc cgagcaaggc caggatgcgt cccgcgatga agtcggcgag gtcgtcgaaa
     5341 tcgcgcggct tgaagtagaa ggccggcatc gccgggacga tcttcgcccc ggcccgtgcg
     5401 gcgcgggtca ggttgtcgag ctggatcagg ttcagcggac actccctcgg cacgatcacg
     5461 agcgggcggg cttccttgag cgtcacgtcc gcggcgcgct cgatcaggtt ggtcgcggag
     5521 ccgtgagcca cacccgacag cgtcttcatc gagcacggga cgatcaccat cgcgtcccaa
     5581 cgctgggagc ccgaggcgat ctcgtcgccg agatcgttcg agcgatgcag ccgaaccgag
     5641 ccggtccggg cgccggcgcc gtagcgatcg tcgagccact gctcgatcgt cgtcgacttg
     5701 aggttcagct cgcattcgtc gatcagcagc cgccggccgt actcgctcgc taccagctcg
     5761 acccgagccc ccagctcgag acaggccttg acgaaacgca cggcgtaaat cgcgccgctc
     5821 gctcccgtca cggcgacgac gatgcgtcgg ccggtggtcg cgtgtcgctg ttccatcgct
     5881 ccccctggcc cctagccccg agccattccc agcgccgcca gcaccgcgac gaggtacagc
     5941 aggctgatca cgccgttcat gttgaagaag gccacgccga cacgggacag gtcgtcggcc
     6001 cgcaccaacc ggtgctccca ggcgagcagc gccgcgatca gcagccacc
//
LOCUS       DASPGN010000162         3937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1549056, whole genome shotgun sequence.
ACCESSION   DASPGN010000162 DASPGN010000000
VERSION     DASPGN010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549056"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3937
                     /locus_tag="VD788_04620"
     CDS             <1..>3937
                     /locus_tag="VD788_04620"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HXV75581.1"
                     /translation="GANLFHSFSDFNVLQGQSATFTSNFAGATDNVISRVTGGNASTI
                     NGLLRSEVAGADFWLINPNGLVFGAEAVLDVPAGFHASTADVLELADGGSFRATDPGA
                     SSLTIAPPSAFGFLDATVGTIAVNGSQLSVGAGESLALVGGRQTLTNASLAAPSGRVD
                     LVGVASAGSVATTAGVGGVLGVSGFDAFADIDLSASTVSVSTAGGTDAGAIGVVGQFV
                     TLLSGSSLLAETASTGNGGSIAVEAGTGGLGLLQASRVSVSSLSTAAGAGDAGQITIA
                     GDQVFVEDSAVLARGRAGEGGSIEVDANFVTVLGGSIQSESTDIARSGSVDIEGSLTV
                     APDGSVRAGDVVSVSGDSVSLFQGRIDAMDIRLAVNSLSMNAGSAMGGTSGTAQLNLT
                     GVDGGIAEFVSLFDESFIAAETTMTAKDISLGDNTSIGVAGAAIVLNVDRLFLSSGGS
                     ISVGSRDGIDGGTVLIRGASADAAELVSIRGVSSSGRRSSVDTGAGVNPFIDDSTTGG
                     PITIIARSLELTDEGRITSLASSLGSGGDILLQVDSLTMDRGAVISSGTGAFGDAGDI
                     TIRGATAPAAALVALEGGDGENGTGIFSASTFRDAGPGGPQGVAGAAGNISITADEIR
                     LSSTQFGIPLIDASTVEGPGGSISLGVGTLTVSGGFIQAFTTGPGDAGSISIAGTDGG
                     AADLIVLNFLPSDITTSSLSNAEDAGDAGAISIAAREIQVRGGASISSETSGGGAGGS
                     IGLQVDELILAGEESRINVSTRGSGDAGDVEIDGLDGAPVSRIEISGLSAGVSSSSAR
                     IFGSPADLGDAGNISIRADSLSLTGLGQLAARTDGGAGGTIDIAVRTLSVTDGASIDA
                     RATADGDAGDIVIQGVDGGRADSVVVAGISESGVGNVSARITSSSGGAGPDAGAAGNI
                     DIASELVTIADGGFVSGETVDGQGGTIELAVGTLTLTGGSIRAATLGTGNAGSIAITG
                     FDGDAADRVELGGFPSEITSSSVSSGEDGGDAGTITIAAREIAVLQGSRIASDTFGGG
                     DGGSIAINVDDLSLVGLESSIAVGTISSGNAGNIVIDGIDGAAANSVLLSGEGAAIRS
                     DATRIFGSPAQIGAAGSIAIRADSVTLEDLAQVSVLTSGSSGGAIDFGVETLAISGGA
                     GIDARTLESGDAGDISIHGIDGGRASEVTITGVSDRPNASGTPSRISSASQGTGPGAG
                     AAGNISIAAESVIVSDGGVISAATLDGQGGAISMDVGQLTIADGGTVTTAVSGAGTGG
                     SIGVVADGMVLLDAGRMIADSSAAGPSGSISVTADELRLENGAALSVRST"
BASE COUNT          589 a         1302 c         1439 g          607 t
ORIGIN      
        1 tcggcgcgaa cctgttccac agcttctccg acttcaacgt gctccagggc cagtccgcga
       61 ccttcacgtc gaacttcgcc ggggcgacgg acaacgtgat cagccgggtg accggcggca
      121 acgcttcgac catcaacggc ctgctgcgga gcgaagtcgc gggcgcggat ttctggctga
      181 tcaacccgaa cggcctggtg ttcggggccg aggcggtgct cgacgtgccg gcaggcttcc
      241 acgcgtccac cgccgacgtg ctcgagctcg cggacggcgg ctcgttccgc gcgaccgacc
      301 ccggtgcgag cagcctgacg atcgcgccgc cttccgcttt cgggtttctc gatgcgacgg
      361 tggggaccat tgcggtgaac ggatcgcagc tgtcggtagg cgcgggcgag tcgctcgccc
      421 tcgtcggcgg ccggcagacc ctgacgaatg cgtcgcttgc ggcgccgtcc gggcgcgtcg
      481 acctcgtcgg cgtcgcctcc gccggatccg tcgcgacgac agctggagtc ggcggggtgc
      541 tcggcgtttc cggcttcgat gcttttgccg acatcgatct cagcgcatcg acggtcagcg
      601 tcagcaccgc gggcggcacg gacgcgggcg caatcggcgt cgtgggtcag ttcgtaacgc
      661 tcctgtcggg gtcgtcgttg ctcgcggaga ccgcaagtac gggcaacggc ggctcgatcg
      721 ccgtcgaggc cggcaccggc ggcctcggcc tgctgcaggc gagccgggtt tcggtcagtt
      781 ccctgtcgac ggccgcgggt gcgggcgacg ccgggcagat cacgatcgca ggcgaccagg
      841 tgttcgtcga ggattccgcc gtcctggcgc gcggaagggc cggcgagggc ggttcaatcg
      901 aagtcgatgc caacttcgtc acggtgctcg gcggcagcat ccagagcgag tcgaccgaca
      961 ttgcgcgctc gggaagcgtt gacatcgagg gttcgctgac ggtggcgccc gacggaagtg
     1021 tccgtgccgg cgatgtcgtc agcgtgtcgg gtgactcggt ctcgctgttc cagggccgga
     1081 tcgacgccat ggacatccgt ctcgccgtca actcgctgtc catgaacgct ggatccgcga
     1141 tgggcgggac ttcggggacc gcgcagctga acctgaccgg cgtcgacggc ggcatcgcgg
     1201 agtttgtgtc gctgttcgac gaatcattca tcgccgccga gacgacgatg accgcgaagg
     1261 acatttcgct cggcgacaat acgtcgatcg gcgtggcggg cgcggcgatc gtcctcaacg
     1321 tcgaccgcct gttcctgagc agcggcggct cgatcagcgt gggttcccgt gatggcatcg
     1381 acggcggtac cgtcctgatc cggggcgcgt cggcggacgc ggcggagctg gtctcgatac
     1441 ggggcgtctc ttccagcggc cgccgcagca gcgtggatac cggcgcgggc gtcaatccgt
     1501 tcatcgacga ctccaccacc ggcggcccga tcaccatcat cgcccgaagc ctggagctga
     1561 cggacgaggg ccggatcacg tcgctcgcca gctcgctcgg atcgggcggg gacatcctcc
     1621 tgcaggtgga ttcgttgacc atggatcgcg gcgcggtgat ttcgtcaggc acgggcgctt
     1681 tcggggatgc cggggacatt acgatccgcg gcgcgacggc tcccgccgcg gcgctggtcg
     1741 cgctcgaagg cggggatggc gagaacggca ccggaatatt ctcggcctcg accttccggg
     1801 acgccggtcc cggcggcccc cagggcgtcg ccggcgccgc gggcaacatc tcgattaccg
     1861 cggacgagat tcggctcagc tcgacgcagt tcggcattcc gttgatcgac gcctcgaccg
     1921 tcgaaggccc tggcggctcg atctcgctcg gcgtgggcac gctgacggtc agcggcggat
     1981 tcatccaggc tttcacgacg gggccgggcg acgccggctc gatctcgatc gccggtaccg
     2041 acggcggcgc ggcggacctc atcgtgctca atttcctgcc gagcgacatc acgaccagct
     2101 cgttgtcgaa cgcggaggac gcgggcgacg ccggagccat cagcatcgcg gcgcgcgaga
     2161 tccaggtacg cgggggcgcc agcatttcct cggaaacgtc cggaggcggt gccggcggat
     2221 cgatcggcct gcaggtcgac gaactgatcc tcgcgggcga ggagtccagg atcaacgtca
     2281 gcacgagggg cagcggggat gccggtgacg tcgaaatcga cggcctggac ggtgcgccgg
     2341 tcagccgcat cgagatttcg ggcttgtcgg ccggcgtgtc cagctcgtca gcgcggatat
     2401 tcggcagccc cgcggatctc ggcgacgcgg gcaacatctc gatccgggcc gactcgctgt
     2461 cgctgaccgg gctcggccag ctggcggcgc gaaccgacgg cggtgctggc ggaacgatcg
     2521 acatcgcggt gaggacactg tccgtgaccg atggtgcatc catcgatgcg cgggcgacag
     2581 ccgacggcga tgcgggcgac atcgtcattc aaggcgtcga cggcgggcgc gcggacagcg
     2641 tcgtcgtggc gggcatcagc gaatcgggcg tcggcaacgt gagcgcacgc atcaccagca
     2701 gttccggcgg agccggaccg gacgctggcg cggcgggcaa catcgacatc gccagcgagc
     2761 tcgtcaccat cgcggacggc gggttcgtca gcggcgaaac cgtcgacggc cagggcggta
     2821 cgatcgaact ggcggtcggc acgctgacgc tgacgggcgg cagcatccgg gcggcgacac
     2881 tcggtacggg gaatgccgga tcgatcgcca tcacgggttt cgatggtgat gcggcggacc
     2941 gggtcgagct cggcggcttc cccagcgaga tcaccagcag ttcggtgtcc tccggcgagg
     3001 acggcggcga cgcgggaacg atcacgattg cggcccggga gatcgccgtc ctgcagggtt
     3061 cgcggatcgc ctccgacaca ttcggcggcg gcgacggcgg ctccatcgcg atcaacgtcg
     3121 acgacctgag cctcgtgggc ctggagtcgt cgattgccgt cggcacgatc agcagcggca
     3181 atgccggaaa catcgtgatc gacggcatcg acggcgcagc ggcgaacagc gtcctgctgt
     3241 ccggcgaggg cgccgcgatc cgcagcgacg cgacacgtat tttcggcagc ccggcgcaga
     3301 tcggggcggc cggcagcatt gcgattcgcg cagattcggt gacgctcgag gacctcgcgc
     3361 aggtatcggt cctgaccagc ggcagctcgg gcggtgcgat cgacttcggc gttgaaacgc
     3421 ttgcgatcag cggcggcgcc ggcatcgacg cgcgcacgct ggaatcgggc gacgccggcg
     3481 acatcagcat ccacggcatc gacggcggcc gggcatccga ggtcacgatc acgggcgtct
     3541 ccgatcgccc caacgcgagc ggcaccccct cgcgcatctc cagcgcttcc cagggcacgg
     3601 gtcccggggc aggtgcggcc ggcaacatct cgattgccgc ggaatcggtc attgtcagcg
     3661 acggcggggt catcagcgcc gcgaccctcg acggccaggg cggggcgatc tcgatggacg
     3721 tcgggcaatt gacgatcgcg gacggcggca cggtgacgac ggccgtctcg ggtgcgggca
     3781 cgggcggaag catcggcgtc gtcgccgacg ggatggtgct gctcgacgcc ggccggatga
     3841 tcgccgattc gtcggcggcc ggcccctccg gcagcatttc ggtcaccgcc gacgagctgc
     3901 ggctcgagaa cggcgccgcg ctgtcggtcc gctccac
//
LOCUS       DASPGN010000163         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_613078, whole genome shotgun sequence.
ACCESSION   DASPGN010000163 DASPGN010000000
VERSION     DASPGN010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613078"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VD788_04625"
     CDS             complement(<1..438)
                     /locus_tag="VD788_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75582.1"
                     /translation="MRRRSSLVALLALAALTLVAPAGAEDDEPRAEVRALTDGIRDDL
                     VQMNFDQALAAIDTLLARPDLTDGERGEVLVLRAQAHGAFGDFDAVEQDYAEILALRP
                     GYVPDPGLTPDKAMARFDKVHKRTIGQVRVEVVPEDAKLRVDGV"
     gene            complement(435..1913)
                     /locus_tag="VD788_04630"
     CDS             complement(435..1913)
                     /locus_tag="VD788_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75583.1"
                     /translation="MSRRLGAELRLGVMIGWALVGPVVAGTPEEAERQYRVARRLAAD
                     GSADARVALQKVIELDPGGPLVDDALVEQALLERLPRWPEELGAIDPASCARALELLD
                     RAIDGQADGDRLPEARYYRALLHLEPLPTHDAARARIDLITVATDPRPSDWSHAARYA
                     GGWLAAATGRRERAESAYGRLLVDAAGSLAATRAAVALARSRLRDGDPGGAALLLDRA
                     LREGVDERLEARALRELAVRLALGGAAALPGDARTVIASTGVRALSAAAPTARGVLVA
                     DARSGAVLEFGGDGRAIGSWSVPQASALAVDRSGRVFAANHESVLRLRAGGATSAVAS
                     VGEFASPAAMAVDPLGRVWLLDRRGDRVGKIEPGAALPEPYWTSKEHKLASLALDGTT
                     LVAIDTRGRDVVTLDGTNGVERAGAPDLQRPLAVAVDAAGRVAVLDAKGPAVRLHASG
                     GQPWVTIPCKPAGVERPTAVGFAADGALQLFDESTGGWVRLR"
     gene            complement(1910..>2486)
                     /locus_tag="VD788_04635"
     CDS             complement(1910..>2486)
                     /locus_tag="VD788_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75584.1"
                     /translation="DLDLCRSNRIVLRAAGFVEASIEIPDAATPIEARTLLSSLALDP
                     VPMGTLVFPETPVKLSYYLDGERVDTASGPLELPQGTHRLRATNDRYWIDVSRDVEIS
                     AGSEVEAAVAVPALTELVVFAYPPNAKVELRHPGGRWRYLDDVPLRYRIAAGRYEVRV
                     TLKTTGETRTSEIVLEPGDNPEVRVSFGTAS"
BASE COUNT          338 a          949 c          891 g          308 t
ORIGIN      
        1 gaccccgtcg acacgcagct tcgcatcctc gggcacgacc tcgacccgca cctgcccgat
       61 cgtccgcttg tggaccttgt cgaagcgggc catcgccttg tccggcgtca gacccgggtc
      121 cgggacgtag cccggacgca acgcaaggat ctcggcgtag tcctgctcga cggcgtcgaa
      181 gtcgccgaat gccccgtgcg cctgcgcgcg cagcaccagc acctcgccgc gttcgccgtc
      241 ggtgagatcg ggcctcgcga gcagcgtgtc gatcgcggcc agcgcctggt cgaagttcat
      301 ctgcacgagg tcgtcccgga tgccgtcggt cagcgcgcgc acctcggccc gaggctcgtc
      361 gtcctcggcg ccggccggtg cgacgagcgt cagcgcggcc agcgccagca gtgcgaccaa
      421 gctcgagcgc cgtctcatcg caggcgcacc catccgccgg tcgactcgtc gaagagctgc
      481 agcgccccgt ccgcggcgaa accgacggcc gtgggccgtt cgacgccggc cggcttgcac
      541 gggatcgtga cccacggctg cccgcccgac gcgtgcaacc gaaccgccgg ccccttggcg
      601 tcgagcacgg cgacgcgacc ggcggcatcg acggcgacgg cgagcggtcg ttgcaggtcg
      661 ggcgcgccgg cgcgctcgac gccgttcgtc ccgtccaacg tcacgacgtc gcgcccccgc
      721 gtgtcgatgg cgacgagcgt ggtcccgtcg agggcgagcg aggccagctt gtgctccttc
      781 gaggtccagt acggttcggg cagcgccgcg cccggctcga tcttgccgac tcggtccccg
      841 cgccggtcga gcagccagac cctgccgagc gggtcgaccg ccatcgccgc gggtgaggcg
      901 aactcgccga ccgaggcgac cgccgacgtg gccccgccgg cccgtaaacg aagcaccgac
      961 tcgtgattgg ccgcgaagac ccgccccgaa cgatcgaccg cgagcgccga agcctgcggc
     1021 acgctccacg agccgatggc ccggccgtcg ccgccgaact cgagtaccgc tccgctgcgc
     1081 gcgtcggcga cgagcacccc gcgcgcggtc ggcgccgccg cggacagcgc gcgcacgccg
     1141 gtcgaagcga tgacggtcct cgcgtcgccc ggcagcgcgg ccgccccgcc gagcgcgagg
     1201 cgcaccgcga gctcgcgcaa cgcccgcgcc tcgagccgct cgtcgacacc ctcgcgcagg
     1261 gcgcgatcga gcaggagcgc ggcgcccccg gggtcgccgt cacgcagacg cgaccgcgcc
     1321 agcgcgaccg ccgcacgggt cgccgccagc gagccggcgg cgtcgaccag cagtcggccg
     1381 taggccgatt cggcccgctc gcggcgcccg gtcgccgccg ccagccagcc gccggcgtag
     1441 cgcgcggcgt gggaccagtc ggacgggcgc gggtcggtcg ccaccgtgat cagatcgatc
     1501 ctggctcgcg ccgcgtcgtg ggtcgggagc ggctcgaggt ggagcagcgc gcggtagtag
     1561 cgggcctcgg gcagacggtc gccgtcggct tgtccgtcga tcgcgcggtc gagcagttcg
     1621 agcgctcgcg cgcaggaggc cgggtcgatc gccccgagct cctcgggcca gcgcggcaat
     1681 cgctcgagca gcgcctgctc gaccagtgcg tcgtcgacga gcgggccccc ggggtcgagc
     1741 tcgatcacct tctgcagtgc cacccgcgca tcggccgacc cgtcggccgc cagccggcgc
     1801 gcgacccgat actgccgctc ggcctcctcc ggcgtgccgg ccacgaccgg ccccacgagc
     1861 gcccagccga tcatcacgcc gagccgaagc tccgcaccga gccgtcgact cacgaggcgg
     1921 tcccgaacga aacgcgcacc tccgggttgt cacccggctc gagcacgatc tcgctcgtgc
     1981 gggtctcgcc ggtcgtcttg agcgtcacgc gaacttcgta gcggccggcg gcgatccggt
     2041 agcgcagcgg cacgtcgtcg aggtagcgcc agcgtccacc ggggtgccgc agctcgacct
     2101 tggcgttggg cgggtaggcg aagacgacga gctcggtcag cgccggcacg gcgacggcgg
     2161 cctcgacctc ggacccggcg ctgatctcga cgtcccgcga gacgtcgatc cagtagcggt
     2221 cgttggttgc gcgcaagcgg tgcgtcccct ggggcagctc gagtggcccg ctcgccgtgt
     2281 cgacacgctc gccgtcgagg tagtagctga gcttgaccgg tgtctcgggg aagaccaagg
     2341 tccccatcgg gaccgggtcc aacgccaaag acgaaagcag cgtgcgggct tcgatcggcg
     2401 tggcggcgtc ggggatctcg atcgaggcct cgacgaagcc ggccgcgcgc agcacgatcc
     2461 gattcgagcg gcacagatcg aggtcg
//
LOCUS       DASPGN010000164         8367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_729814, whole genome shotgun sequence.
ACCESSION   DASPGN010000164 DASPGN010000000
VERSION     DASPGN010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729814"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VD788_04640"
     CDS             complement(<1..334)
                     /locus_tag="VD788_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75585.1"
                     /translation="MTAVAAERRSGGALAGVVAAIAVAASLLAPALSAGFYGDDFSLW
                     HEGRRLIAEPAKFFVGPSNFYRPANSWLFAAHHLAFGTDPRGYHAGTLLMHLACGGLL
                     GYLVSRFVR"
     gene            450..3308
                     /locus_tag="VD788_04645"
     CDS             450..3308
                     /locus_tag="VD788_04645"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HXV75586.1"
                     /translation="MRRTTVCVVLGGLIGAWAGDCAARVTRVEIGRTEEVLDGKPFGS
                     VGPYEKLVGTVHFAVDPGDPANAAVVDLDLAPTNAGGEVTFRADLYLLRPKRAERGNG
                     TLLVEVANRGGKGVLTLLNGAVGSLDPTGVEHFGDGFLLRRGFSVAWVGWQFDVRDET
                     ELVRLDPPAATGRSGLVRSDFVVAERATFQPWGHVIAGRIGGTEYPVADPDDEQNVLT
                     VRDHPTAERRTIDRRQWSFARDGGTINGIDHPAGFEPGRIYELIYRAADPAIAGLGLA
                     AIRDFASYAKHDETSLVRADRAIAFGISQSGRFLRQFLHQGFNIDERGRSAFDGVIAH
                     TAGAGRGSFNHRFAQPSRDAQSLEALFYPTDLYPFADVPLVDPLSGREEGLLDRLAPP
                     PDPPKIFYTNTSYEYWGRAASLIHTSPDGAVDARIPDNVRIFLLAGLQHFSRPLPPAR
                     VSLPQIRGRYVSNPNPSSWTMRALVVAMQRWVALGEPPPESAYPRIDEGTLVPVETVK
                     DEFPEIPGVELPREAQLAYRIDYGPDWRSSGVVSEQPPEVGRPFAVLVPAVDADGNDR
                     GGVRIPQLETPLATYTGWNLRDPSIGAPSERVSFLGSYFPFPVDEQSRQAAGDPRPSI
                     GERYASFDVYRSRYTEAAMQSIARGLLLAEDLDDLIELGKREWHFATRGIELPWSMSS
                     DALARLERTRRDMRFAYGNPPFRFGELRLPGGDGPFPVAVILHGGCWLADYDLRHVGA
                     LADAITAEGIASWTIEYNRVGDPHGGWPGTFLDVAAGADYLRELARDHPLALDRVIAV
                     GHSAGGQLALWLAARPSIPHGSELHAPDPLVLRGVLGLAAAADLDLLHEREVCGGVID
                     ELIGGGPDAFAERYDAASPARSVPRTPQILLNGALDPFWSSVANQYVEAARRAGADVR
                     VIDAPESGHFEMIDPASTTWPLVRDALRELIGWSVAAD"
     gene            3462..4178
                     /locus_tag="VD788_04650"
     CDS             3462..4178
                     /locus_tag="VD788_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75587.1"
                     /translation="MRRHICLIATTMISFAALESETRAEHPYPPRLPENAFQVRLGVF
                     APDGGGTLWDDLDQRFALGPSDFNGGAWGLSFVGGINEHFEVGLNADWYGRTVTTADP
                     LYVDTDGFPIVHDTTFRQFPVGVDLRLVPTGRRPGKPVVFVGAGGGLSFWEYREVGDF
                     VDESDPLLPVYFGEFHDSGQSLEGRLLAGIEFPITPAFNLTFEGRYSFGETELEGDLA
                     GLGTIDRGGLWVFAGAGFRF"
     gene            4219..5700
                     /locus_tag="VD788_04655"
     CDS             4219..5700
                     /locus_tag="VD788_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXV75588.1"
                     /translation="MGGRLDSILDAIGRTPVIRINRLAPDGAELWVKVEGANPLGSVK
                     DRLALGVIEAAERSGELSPGQTVIEATSGNTGIGLAMVCAAKGYPLVVTMAENFSVER
                     RKIMRFLGARVVLTPSTMRGLGMLAKAEELAEKHGWFLCRQFENEANADVHSRTTARE
                     ILEQFAGEPLDYWVTGFGTGGTLKGVARVLRHERPATQIVVCEPDNSPMLQSGVRQPR
                     RADGTPVDSHPSFRTHLVQGWAPDFIPKLTEDVVDAGMIDRIVSVNSGDAVRSSRELA
                     CKEGIFTGISGGATFAGAVELCRSIPRGSTVLCMLPDTAERYLSTPLFDDIAVEMTEE
                     EIEIARSTPNYRFDVCPAPTRETAPRPLTPEAVEFVARTISAAGDPVVVFALEWCEFC
                     WSVRKLLDRCGVSYRLVELDSAEYLRDDWGGRVRAALLDRTSSRTLPQVFVGGEYVGG
                     CTELFDAYASGDLQTRLDRAGVGYDRGARVDPHSLLPAWLHPR"
     gene            5842..6381
                     /locus_tag="VD788_04660"
     CDS             5842..6381
                     /locus_tag="VD788_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75589.1"
                     /translation="MNARLLLTACALLVATLGLAEDHEHAAAPKVDDARFQFLEKLAG
                     QWVTRSATGAMPAGLFEFRVTAGGTAIEEREMIGTPMEMLTVYHLDGGELVGTHYCMI
                     GNQPKVKAAPKVTDNTLAFACNGTPGNAKSHDEHHVHAWSIQLREDGSLLYNAELSEN
                     GTVTEEPSVVLVRQETASR"
     gene            6408..6758
                     /locus_tag="VD788_04665"
     CDS             6408..6758
                     /locus_tag="VD788_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXV75590.1"
                     /translation="MQYLLLIYSNESDEPRPGTAAGDAHLNAYMKFTEDVQQRGVMLG
                     GNALESVKTATTVRVRNGKTDTTDGPFAETKEQLGGYYLLDCEDLDEALELAAKIPSA
                     KIGSVEVRPVMRFD"
     gene            6761..8029
                     /locus_tag="VD788_04670"
     CDS             6761..8029
                     /locus_tag="VD788_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV75591.1"
                     /translation="MERSGDATSDRSRAIVERTIREHWGYILATLVGYVRDLQLAEDV
                     LQDAAVAALEHWPASGVPRHPCAWLLQTARRKAIDRFRRDRRFEGRKAELQRMLELEQ
                     RAAGETEETLVDERLSLIFTCCHPALGETARVALTLRTLGGLTTAEIARAFLVAEPTM
                     HQRLVRAQAKIKTSGIPYRVPPPELWPERLDSVLAVIYLIFNEGYAARSGGEPTRTDL
                     SDEAIRLGRIVVRLAPSEPEAVGLLALMLLHDSRREARTDAAGRLVPLSEQDRGSWNR
                     GRIAEGERLLRRALAAGRAGPYRVQAAISACHATATSHETTDWHEITGLYAKLYELQP
                     SWVVKLNQIVALSFAVGPEAGLRALAPLESVKILHGYQPFHAARADLLRRAGRSGEAA
                     TAYRRALELTTNAAERGFLERRLDGLGTAG"
     gene            8106..>8367
                     /locus_tag="VD788_04675"
     CDS             8106..>8367
                     /locus_tag="VD788_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75592.1"
                     /translation="MRGSSGWAIGWVLLFASGVAVAETAGEVLVLGRDGVNCGEDPHC
                     INRLHPAIPLVATARPGQTIVMHTRNASDFALDPAAAVDSRAG"
BASE COUNT         1271 a         2843 c         2984 g         1269 t
ORIGIN      
        1 agcggacgaa gcggctgacg aggtagccga gcaagccgcc gcaggccagg tgcatcagca
       61 acgttccggc atggtagccc cgtggatcgg tgccgaacgc caggtgatgc gcggcgaaca
      121 gccaggagtt ggccggacgg tagaaattcg acggcccgac gaagaacttc gccggctcgg
      181 cgatcagccg ccgcccctcg tgccacaggc tgaagtcgtc gccgtagaat ccggcggaca
      241 gcgcgggagc caacagcgac gccgcgacgg cgatcgccgc gacgactccc gccagcgccc
      301 ctccgctgcg tcgttccgct gcgaccgccg tcactcgaca ctcctcgatc gaatcccgca
      361 cgacacacac tcgtgccggc cggcgggggc tgtcaagtgc gccgcgccgc gaggcggtcg
      421 ctcgtagtaa ggttgcggcg gaggtcgcga tgcgtcggac gactgtgtgc gtggtgctcg
      481 gcgggctgat cggggcgtgg gccggcgact gcgcggcacg cgtcacgcgg gtcgaaatcg
      541 gtcgcacgga ggaggtgctc gacggcaagc cgttcggttc ggtcggcccc tacgagaagc
      601 tcgtcggcac ggttcatttc gccgtcgatc ccggtgaccc ggcgaacgcg gcggtggtcg
      661 acctcgacct ggccccgacg aacgccggcg gcgaggtgac gttccgcgcc gacctctacc
      721 tgctgcgacc caagcgggcc gagcgcggca acgggacgct gctggtcgag gtcgccaacc
      781 gcggtggcaa gggcgtgctg accctgttga acggtgctgt gggctcgctc gatccgaccg
      841 gcgtggagca tttcggcgac ggcttcctgc tgcgccgggg cttcagcgtg gcgtgggtcg
      901 gctggcagtt cgacgtgcgc gacgagaccg aactggtccg actcgaccca ccggcggcaa
      961 cgggccgctc cggcctcgtt cgctcggact tcgtcgtcgc cgagcgcgcg acgttccagc
     1021 cgtggggaca cgtcatcgcc ggacggatcg gcggcaccga gtatcccgtc gccgatcccg
     1081 acgacgagca gaacgtgctg accgtgcgcg atcacccgac cgccgaacgc cggacgatcg
     1141 accgccggca atggtcgttc gcgcgcgacg gcggcacgat caacggcatc gaccaccccg
     1201 ccgggttcga gcccggtcgg atctacgagc tgatctaccg cgccgcggac ccggcgatcg
     1261 ccggactcgg tctggccgca atccgcgact tcgcatccta tgccaagcac gacgaaacca
     1321 gcctcgtgcg ggccgaccgc gcgatcgcct tcggcatttc gcagtccggg cgcttcctgc
     1381 gtcaattcct ccaccagggg ttcaacatcg acgaacgcgg ccgctcggcc ttcgacggcg
     1441 tgatcgcgca taccgccggg gccggccgcg gcagcttcaa ccatcgcttc gcgcagccgt
     1501 cgcgagacgc acaatcgctg gaggcgctgt tctatccgac cgacctctac ccgttcgcgg
     1561 acgttccgct ggtcgatccg ctgagcgggc gcgaggaggg gctgctcgac cggctggcgc
     1621 cgccgcccga cccgccgaag atcttctaca cgaacacgtc gtacgagtac tgggggcgcg
     1681 ccgcttcgct gatccacacc tcgcccgacg gcgcggtcga cgcgaggatc ccggacaacg
     1741 tccggatctt cctactcgcc ggtctacagc acttctcgcg accgctgccg cccgcgcggg
     1801 tttccctccc gcagatccgc ggtcgttacg tgagcaaccc gaacccgtcg tcgtggacga
     1861 tgcgggcgct cgtcgtcgcg atgcagcgtt gggtcgcgct cggcgaaccg ccgccggaga
     1921 gcgcctaccc gcgcatcgac gagggcacgc tggtgccggt cgagacggtg aaggacgagt
     1981 tcccggaaat tcccggggtc gagctgccgc gcgaggcgca gctcgcctat cgcatcgact
     2041 acgggcccga ctggcgctcc tcgggagtcg tctccgagca gccccccgag gtcggacgac
     2101 cgttcgcagt gctggttccc gccgtcgatg cggacggcaa cgaccgcggc ggtgtgcgca
     2161 tcccccagct cgagacaccg ctcgccacct acaccggctg gaacctgcgc gacccgtcga
     2221 tcggcgcgcc gtccgagcgg gtcagcttcc tcggttcgta tttcccgttc cccgtcgacg
     2281 agcagtcgcg ccaggcggcc ggcgacccgc gcccgtcgat cggcgagcgc tacgcatcgt
     2341 tcgacgtcta ccgcagccgc tacaccgagg cggcgatgca gtcgatcgcg cgcggcctgc
     2401 tgctggcgga ggacctcgac gacctgatcg aactggggaa gcgcgaatgg cacttcgcga
     2461 cgcgcggcat cgagctgccg tggtcgatgt cgtcggacgc gctcgccagg ctcgagcgga
     2521 cgcggcgtga catgcgcttc gcctacggca acccaccgtt ccgcttcggc gagctgcgtc
     2581 ttcccggcgg cgacgggccg ttccccgtcg ccgtgatcct gcacggcggc tgctggcttg
     2641 ccgactacga cctgcggcac gtcggcgcgc tggccgacgc gatcaccgcc gagggtatcg
     2701 ccagctggac gatcgagtac aaccgcgtcg gcgatccgca cggcggctgg cccggtacgt
     2761 ttctcgacgt cgccgccggc gcggattacc tgcgcgagct ggcgcgcgat cacccgctcg
     2821 cgctcgaccg cgtgatcgcg gtcggtcact ccgcgggcgg ccagctcgcg ctgtggctcg
     2881 ccgcgcggcc gtcgatcccc cacggctccg agctgcacgc accggacccg ctcgttctgc
     2941 gcggcgtgct ggggctggcg gcggcggccg acctcgacct gctgcacgag cgcgaggtgt
     3001 gcggtggcgt catcgacgag ttgatcggcg gtgggccgga cgcgttcgcg gagcgctacg
     3061 acgccgcgtc gcccgcgcgg tcggtgccgc gtacgccgca gatcctgcta aacggcgcgc
     3121 tcgatccgtt ctggtcgtcc gtcgccaacc agtacgtcga ggcggcgcgc cgcgccggtg
     3181 ccgacgtgcg ggtgatcgac gctcccgagt ccggtcactt cgagatgatc gatcccgcgt
     3241 cgacgacttg gccgctggtg cgcgacgcac tgcgcgagct gatcgggtgg agcgtcgcgg
     3301 ccgactgatc ggctggtcgc cgcgggacgt cggtgtcctt cgtcggggac atcgggcgac
     3361 gatcgctgcg gcgaaagcgg gcgatttccc atcgtcgcgg tctggcctcg cgcttgcagt
     3421 gcggacggcc gacaaccaac cgggggcggc cggggtgcac gatgcgacgt cacatctgct
     3481 tgatcgcgac cacgatgatc tcgtttgccg cgctcgagag cgagacccgt gcggagcacc
     3541 cctatccgcc gagactgccg gagaacgcgt tccaggtccg cctcggcgtc ttcgcgccgg
     3601 acggcggcgg cacgctgtgg gacgacctcg accagcgatt cgccctcggc ccgagcgact
     3661 tcaacggcgg ggcctggggt ctgagcttcg tcggcgggat caacgagcac ttcgaggtcg
     3721 ggctgaacgc cgactggtac ggacggaccg tcaccacggc cgacccgctc tacgtcgaca
     3781 ccgacgggtt cccgatcgtc cacgacacga cgttccgcca gtttcccgtg ggagtcgatc
     3841 tgcgcttggt cccgaccgga cgccggccgg gcaagccggt cgtcttcgtc ggtgcgggcg
     3901 gggggctgag cttctgggag taccgggagg tcggtgactt cgtcgacgag agcgacccgc
     3961 tgcttccggt ctacttcggc gagttccacg acagcggcca gtcgctcgag ggccgcctgc
     4021 tggcgggcat cgagttcccg atcacgccgg cgttcaacct gaccttcgag ggccgctaca
     4081 gcttcggcga aaccgagctc gagggcgatc tcgccggcct ggggacgatc gaccgcggag
     4141 gcctgtgggt cttcgccgga gcggggttcc gcttctgacg cgtttgccgc tatcatcgtt
     4201 tgtcttcggg gcacgacgat gggcggcaga ctcgacagca tcctcgacgc gatcgggcga
     4261 accccggtga tccgcatcaa ccggttggct cccgacggag ccgagctgtg ggtcaaggtc
     4321 gaaggtgcga acccgctcgg ctcggtcaag gaccggctgg cgttgggggt catcgaggcc
     4381 gccgagcgca gcggggagct gtcgccgggc cagacggtga tcgaggcgac cagcggcaac
     4441 accggcatcg gcctggcgat ggtctgcgcc gcgaagggct atccgctcgt cgtgacgatg
     4501 gcggagaact tcagcgtcga gcgccgaaag atcatgcgct ttctcggtgc gcgcgtcgtg
     4561 ctgacaccgt cgacgatgcg cggcctgggc atgctggcaa aggccgagga gctcgccgag
     4621 aaacacggct ggttcctctg ccgccagttc gaaaacgagg ccaacgccga cgtgcactcg
     4681 cgcacgaccg cgagagagat cctcgaacag ttcgccggcg agccgctcga ttactgggtg
     4741 accggcttcg gcaccggcgg gacgctcaaa ggggtcgcac gggtgctgag gcacgagcgt
     4801 cccgctacgc agatcgtcgt ctgcgagccg gacaactcgc cgatgctgca gagcggcgtg
     4861 cggcagccgc gtcgggccga cgggacgccg gtcgacagcc acccgagctt ccgcacgcac
     4921 ctggtccagg ggtgggcgcc cgacttcatt cccaagctga ccgaggacgt cgtcgacgcc
     4981 ggcatgatcg atcggatcgt atcggtcaac agcggcgacg cggtccggtc gagccgtgag
     5041 ctggcttgca aggaggggat cttcaccggt atttccggcg gagccacctt tgccggcgca
     5101 gtcgagctgt gtcgttcgat ccctcggggg tcgaccgtgc tgtgcatgct gcccgacacg
     5161 gcggaacggt acctgagcac gccgctgttc gacgacatcg ccgtcgagat gaccgaggag
     5221 gagatcgaga tcgcgcgctc gacgccgaac taccgcttcg acgtttgtcc ggcaccgacg
     5281 cgggagaccg ctccccggcc gcttacgccg gaggccgtcg agttcgtcgc gcgcacgatc
     5341 tcggcggccg gcgacccggt cgtcgtcttc gcgctcgagt ggtgcgagtt ctgctggtcg
     5401 gtgcgcaagt tgctcgaccg ttgcggggtc tcctatcgct tggtggagct ggactcggcc
     5461 gagtatctgc gcgacgactg gggcggcagg gtccgcgccg cgttgctcga ccgcacgtcg
     5521 tcgcgcacct tgcctcaggt cttcgtcggc ggcgagtacg tcggcggctg caccgagctg
     5581 ttcgacgcgt acgcgtcggg cgacctgcaa acgcggctcg accgcgccgg agtcggctac
     5641 gaccgcggcg cgcgcgtcga tccgcactcg ctgctgccgg cctggctcca cccgcgctga
     5701 ggcgggaagc ggcgggtcgc ccccggcggg ccaaaaaaaa attcgcgccg gatgtcgaat
     5761 cggtcgatgg tccggcgtct tgctgtcgaa ggcgccggga agaccccggc ccgacgaacc
     5821 gacaaccgaa ggagagcgac gatgaatgct agattgctac tgacggcctg cgcgctgctg
     5881 gtggccacgc tcgggctggc cgaggaccac gagcacgccg cggcgccgaa ggtcgacgac
     5941 gcccggttcc agttcctcga gaagctcgcc ggccagtggg ttacccgctc ggcgacgggc
     6001 gcaatgccgg cgggcctgtt cgagttccgc gtgacggccg gcgggacggc gatcgaggaa
     6061 cgcgagatga tcggcacgcc gatggagatg ttgacggtct atcacctgga cggcggcgag
     6121 ctggtcggga cgcactattg catgatcggc aaccagccga aggtgaaggc ggcaccgaag
     6181 gtgaccgaca acacgctcgc gttcgcctgc aacggcacgc cgggcaacgc gaaatcgcac
     6241 gacgagcacc acgtccacgc ctggtcgatc cagttgcgcg aggacggctc gctgctctac
     6301 aacgccgagc tgagcgagaa cggcacggtg accgaggagc catcggtggt tctcgtacgg
     6361 caagaaaccg cgagccgctg acacggcgtc gccaggagag aacaaccatg cagtacctgt
     6421 tgttgatcta cagcaatgag agcgacgagc cgcgtcccgg gaccgcggcg ggcgacgcgc
     6481 acctcaacgc ctacatgaaa ttcaccgagg acgttcaaca gcgtggcgtg atgctcggcg
     6541 ggaacgcgct cgagagcgtc aagacggcga cgaccgtccg cgtgcggaac ggcaagaccg
     6601 acacgaccga tggcccgttc gccgagacga aggaacagct gggcggctac tatctgctcg
     6661 actgcgagga tctcgacgag gcattggagc tggcggccaa gatcccgtcg gccaagatcg
     6721 gctcggtcga ggtgcgtccg gtgatgcgct tcgactgatc gtggagcgct cgggggacgc
     6781 gacgtcggat cgctcgcggg cgatcgtcga acggacgatc cgtgagcatt gggggtacat
     6841 cctcgccacc ttggtcggct acgtgcgcga cctgcagttg gcggaggacg tcctgcaaga
     6901 tgccgccgtc gcggccctcg agcattggcc ggcgagcggt gtgccgcgtc acccgtgcgc
     6961 ctggctgctg cagaccgcgc ggcgcaaggc gatcgaccgg ttccggcgcg accgccggtt
     7021 cgagggcagg aaggccgagc tgcagcggat gctggagctc gagcagcgag cggccggcga
     7081 aaccgaagag acgctggtgg acgaacgtct gtcgctgatc ttcacctgct gccatcctgc
     7141 gctcggcgaa acggcacggg tcgcgctgac gctgcgcacg ctcggcggtc tgacgacggc
     7201 ggagatcgcg cgagcgttcc tcgtcgccga gccgacgatg caccagcggt tggtacgcgc
     7261 gcaggccaag atcaagacca gcggcatccc ctaccgcgtg ccgccgcccg agctgtggcc
     7321 ggagcggttg gactcggtgc tggcggtcat ctacctcatc ttcaacgagg gctatgcagc
     7381 gcgctccggc ggcgagccga cacgaacgga cctgagcgac gaggcgatcc gtctcgggcg
     7441 aatcgtcgtc cggctcgcgc caagcgagcc cgaggcggtc ggtctgttgg cgctgatgct
     7501 gttgcacgac tcgcgtcgcg aggcccggac cgatgccgcc ggccggctgg ttccgctgtc
     7561 cgaacaggac cgcggcagct ggaatcgcgg acggatcgcc gaaggagaac ggctgctgcg
     7621 gcgcgccctg gcggcggggc gcgccggacc gtaccgagtc caggcggcga tcagtgcctg
     7681 ccacgccacg gcgaccagtc acgaaacgac cgattggcac gagatcaccg ggttgtacgc
     7741 caagctgtac gagttgcagc cgtcgtgggt cgtcaagctg aaccagatcg tcgcactatc
     7801 attcgccgtc ggccccgaag ccgggctgcg ggcgctcgcg ccgctcgaat cggtgaagat
     7861 tctccacggc tatcagccgt tccacgccgc gcgtgccgat ctgctgcgtc gcgcggggcg
     7921 aagcggtgag gcggcgacgg cgtaccgccg cgcgctggag ctgacgacca acgccgccga
     7981 gcgcggcttc ctcgaacgtc ggctggacgg actcggaacg gcgggttgat ccgcggccgg
     8041 aacgttggat cggaccggcg actcgtgtaa ggtcggtgcg aggcgcgctg ccggcggggg
     8101 atgtcatgag gggatccagc ggttgggcga tcgggtgggt gctgctgttc gcgagcgggg
     8161 tggccgtcgc ggaaacggcc ggggaggttc tggtgctcgg gcgcgacggg gtgaactgcg
     8221 gcgaggaccc gcactgcatc aaccggttgc atccggcgat cccgctggtc gcgaccgcgc
     8281 gtcccgggca gacgatcgtc atgcacacgc gtaatgcgag tgacttcgcc ctcgatcccg
     8341 cggccgcggt ggactcgcgc gcaggcg
//
LOCUS       DASPGN010000165        13653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1549384, whole genome shotgun sequence.
ACCESSION   DASPGN010000165 DASPGN010000000
VERSION     DASPGN010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 13653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13653
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549384"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VD788_04680"
     CDS             complement(<1..381)
                     /locus_tag="VD788_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase"
                     /protein_id="HXV75593.1"
                     /translation="MAERSALIIGTGMSVPARVLTNKELETMVDTSDEWITSRTGIRE
                     RRVAAAGDSLKQYCVEASRRAMEAAGVEPEQIDLIILATVTPDMPLPATACTVQHALG
                     CGRAAAFDLSAGCSGFIYAQSVAKQ"
     gene            complement(534..716)
                     /gene="rpmF"
                     /locus_tag="VD788_04685"
     CDS             complement(534..716)
                     /gene="rpmF"
                     /locus_tag="VD788_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXV75594.1"
                     /translation="MPNPKRRHSKARRDKRRAHDALPKVGLSVCPNCHEMKLPHRVCP
                     HCGQYKGREVVTPPEI"
     gene            complement(747..1277)
                     /locus_tag="VD788_04690"
     CDS             complement(747..1277)
                     /locus_tag="VD788_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HXV75595.1"
                     /translation="MLIDIHKVEPEGIRFDTSIHLAPLPLEGPEQVEVLQAHVHGDLR
                     RGADGVDFRGALEARVRMSCNRCLDAVELPIAVEFHLILVPNAVELGASDDGSNDRAT
                     LLFHAEGGQARLEEIVREQIYLQVPLKSLCRPDCAGLCPTCGVNRNRLECACRSEETD
                     PRLAPLLELKKKLEQH"
     gene            complement(1342..2832)
                     /locus_tag="VD788_04695"
     CDS             complement(1342..2832)
                     /locus_tag="VD788_04695"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXV75596.1"
                     /translation="MQSHFNSHSLAEIFRDLYISERTGELHLSDEDREARVFFNRGLI
                     DYVDSNEPPHDLGQALVDSGRISAGALLEAQRVVKDPVELPQALLNRGLVAKSSLVTV
                     ARELAEKIIAATFNWDGGVAAFQERDWGVDLLESDVLSTLRLILHGVASIADFDGVIE
                     AMRGLDHELTLRKPVPIPLEKLTLSRTQGFVLSRIDGRTDFRAMISTLPPEEEGAASR
                     FVFGLLILGVAEFRPTLADGPFRVANILRDHADRCALERMQEKTISEAYSRMRKQNPH
                     EILGVTTTASRTAIDRAYAEAKSLFARDRLLPSVRDRFRSELGVIESRLVEAYLNLTQ
                     PARSEKRSEREVDRTPTQNISAEDLLMRPELDKTKRRTEADEAARVGELYYNKARQAM
                     LAGDFHNAIQYGKLAISYSAEDARFYSLVADCQVRNPEARWQRMAEQNYTKATELDPW
                     NAQYWISLGRFYKRRGLHLRARRQFEEALRLVPEHEEATKELAALR"
     gene            complement(2998..3723)
                     /locus_tag="VD788_04700"
     CDS             complement(2998..3723)
                     /locus_tag="VD788_04700"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXV75597.1"
                     /translation="MMRIPRRVTVCALASAIWASSAAADEVVRFADGRVLEVARVERN
                     GDTIRLELLGGGALAVPAWRVDGWSRVERAALPPRGSANRPPEAWRNAAGVFARHIER
                     AATEHRLDPVLLTAVAHTESAFDPAAVSPKGATGLMQLMPETAARLGVADVFDVAQNV
                     DGGARYLSWLLDRFDGNTELALAGYNAGENAVDRYRGIPPYPETERYVEKVLHEADRL
                     RRPAAATAAADLRLTSVAPAVAD"
     gene            complement(3770..5554)
                     /gene="mutL"
                     /locus_tag="VD788_04705"
     CDS             complement(3770..5554)
                     /gene="mutL"
                     /locus_tag="VD788_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HXV75598.1"
                     /translation="MGRIRVLSDALINRIAAGEVIERPASVVKELLENSIDAGARSVS
                     VECAGGGRRMIAVIDDGAGMDRDDALLAIERHATSKLVDDRGLESIATLGFRGEALSS
                     IAAVSRFTLQSAVVDGEGTEVEVHGGRVRSVGEIAMPRGTTIRVERLFFNVPARRKFL
                     RSEATELGHIARLVTNHALARPDRRFRLVHAGRELLHLDPAACARERIGQLYGDELAR
                     RLEPFDSSDGELRVHGFAGRPSEAQTRRDGQHFFVNGRAVKDRVLLHAVGQAYGNTMP
                     RGRHPSLFVFLECAPSDVDVNVHPQKVEVRFRQPVRVHDLVEGVVRSALAGAAVIPRL
                     ADLRPAALDRGRAVAEAISGYADRRSPAADGGPASPAGARPATSGLPFAAAAARPVAD
                     PAMRDTGSPDSAVVPLAQYRRSYILAQDHDGLLLVDQHAAHERVIFERYLAQAVDNHV
                     EVQRLIFPITIELTAADRVTFDDETAEFERLGFRVEPFGGNSIRVTGVPALAGAVDPR
                     ELILELLGEARAVPSTVSAAASLRHRLVTSAACQAAIKVNHTLERQQMQRLLDDLFRT
                     VNPTTCPHGRPLIFRLSHAEIERAFDRR"
     gene            complement(5591..11704)
                     /gene="bamA"
                     /locus_tag="VD788_04710"
     CDS             complement(5591..11704)
                     /gene="bamA"
                     /locus_tag="VD788_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXV75599.1"
                     /translation="MAIVCGLVVAWRQLDEWAWSAVARSLAAAGLRAEVERFHLRPQR
                     FSLEFTNLRVWRVDPSTGRFEASVSYGKLAVPWRYVLGLGRRRVHLAELTLVRPSVVT
                     DGRLLDRSSVRPTASRSFELDVFVDRLQIVGGRWTHEQQDRPLDLRVDDVEIEGGWTR
                     GARSVLGQIGLRAALATPPLEREVELNLASQFRWRRTTIELIDARITGPGVRLHADAT
                     FDLDEQPLLTGTGVIDADLAAVGGLLSGLPRIDGRVEGSVEFTGPDPPKIRGRLQLSD
                     GALDRLAIERAEFALDLEQGALRFREIDARAYGGAVRGSVDAVLGDPASVRVDLEASA
                     LDGRRLARLLDVELPLASAVDARLRLAGRPEDRRSWDGTATFSATGRRAGPGEIPFDA
                     DGTVTIADGSLTVASKTARTIGTRASLRLEAELERTPPSGRLEISGTTDDGALTQTAT
                     ILLLDAIGIDYPEIVRKPISGRGTFEAVVDFGAKTDLSLGLDLRDGMLEDRPYDLLDL
                     ALRIDAEDLAIDHAELSGPGLSIEGSARVGLAPLRLEEIRLVGERIELADLLDLLDLS
                     IDVGGRLSGNVTALQGEAGLDGSGELMLVEANAFGQPIERVTATLRIVGDRFLLEPFD
                     LLSPAGTVSGRGELDQSSRTARFDIDSVEVALERLPGLDGAVEARVVGVGSIEVGQQF
                     VAGQFEIEGQELRTLGIGLGDFSGTLTLDQAGVEARLSSIDARQWGLEAALGWGPGSS
                     IEAVARLDQAVVELPTEAQPAPSAELSGRFAIRGPLTRAPELVIDGAFDHARLHFGPH
                     ALDLAGSAPVRISDGRLQIGPLRLTGEATDLEVESGYDFDGGSIATSIGGTVGIELLS
                     SVLPDVRASGSARIDLRIDGPAGSPTYSGSLALRGGRLRYLGFPHTLEQIEATATFRD
                     QTATIERVRALLGGGELEGRGALRVGASGVESMRFDVSGSNVRVALPAGFVGVYDGEL
                     VLEGTLEQPRLSGDVTMLRGIYNEEFRVGGVFGGGSREYAPTEMGGPLAEMVLDLLVH
                     ADGNVWVRNDVAELESFFDLRIGGTPRRPEIIGRLGLVEGGTLQVRDITYRIRSGTLD
                     FTDIERINPYIQVAAWTAIGGYEITLRVEGTLDALRYDLTSTPSLSQPDIVALLTTGK
                     TLQELAPDGRQTDAEFSGDLAASYFAGALTGRFENQLQQALGLDVLAINPLLIEAGDP
                     TTRVTVGKKVSDDVTAILSADLRSTEDRLYQVEWRANPRMLVTVQRDINGGVGSNVLY
                     TDRFWWRVPRGETVKAVPAEIGQAAEPEPAGRTVGRLTIVGVDEAEAAELVRLLPVEP
                     GAAYSRAEVFRGVERIRSHYVRRGRIKVRVDATTSPEDDPSQPVELVYQVDPGPQIEL
                     EFRGLKPKEQRRLTTQLETMWTESLFADDLYTDAVVRIREDLQQRGFYAVDVRHELHD
                     TGAGETVIFEVDRGELVKVAGITLHGTSAMTAERVRKQMLTRPPSLFGGGVFKPATLR
                     EDLAAIRNLYLDQGYLDVEIDAPRLRLSTDGRRIDISIAIHEGPRQAVVAIRFEGETG
                     VDDKRLEAWSGIRIDEPFSGSALLGGEASLRREFDRLGHPGARVSARLERSDRGVEIV
                     FEIEPGPKVVAAEVRVSGNQLTNERLIRRELAIKQGELVTRDKLLRSQQSLYRLGVFR
                     NVRIEYREAHEIGPGYYVVEVRVEEDRPLTLATGVGYNTEAKFKFSFASAHNNVQGKA
                     RSLGVQGEVSSILSRVQLIGRTPRLFGAAVPGLGNILWEREQKDGFSIERRSTALRVE
                     RILSQRWRGFLRYNFQNVMLLEVTDPAEVAEERVEDIILGDVAIGLLRSTLDNLILPT
                     RGNYFNVNFAVFAEPFLSEADFVLAALTETHTWSYSNGTTFVTTFRVGVAPPFGRTDL
                     VPVSERFFAGGDSTNRGFERDRLGPTAGEISASEGTPLPAGVAADQPVGGEAQLLFNQ
                     EFRFPLLRKLRLKGVLFYDAGNVYFRVSDFDPTDLRHTLGSGLRLETPVGPIRMEYGW
                     KLDRRADESSGVFHLAIGAVF"
     gene            complement(11894..12763)
                     /locus_tag="VD788_04715"
     CDS             complement(11894..12763)
                     /locus_tag="VD788_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HXV75600.1"
                     /translation="MARTVNSKKKAAAKKGQAAQKAKKQPAAARPKPKPAARVTKPKK
                     TGGTRAATTKKAQAGKTASRKAASAKKPKTAGAAVTTKAPTKKAAGAKPTKGKKAGRA
                     AKTAVRRRQLKRFRDVLLARHKSLVEAYHSTKGNTRDSVPDGTEDYIDYAVSSYDRDF
                     SLSLTEMERRQMRLVEEALRRIDRGEFGSCMQCAQEIPEKRLEVEPWARHCIRCQELE
                     EQGLLQDRPFELEDEDEDSSETSSRSRIIDDDSEEEAPDQEVEDDSEDEAPSDAVDTD
                     PAGDEETEDELTV"
     gene            complement(12895..>13653)
                     /locus_tag="VD788_04720"
     CDS             complement(12895..>13653)
                     /locus_tag="VD788_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HXV75601.1"
                     /translation="TAEDQERVAQQVGIDAQVLRDTVNSLHELNPMLGHRGCRLGITF
                     PSIYDMQARAIFEAAAICRKQGIEVHPEVMIPLVGTPEEFQIVAERVHRVAAEVQSKN
                     GGKLPYRVGTMIEIPRACLVADRIAADAQFFSFGTNDLTQMTYGYSRDDVGKFLPFYL
                     EHRVLSADPFQTVDVNGVGRLVRLCIEEARRRSKKLKIGVCGEHGGDARSVIFFHQAG
                     ADYVSCSPYRVPVARLAGAHAALAAGQGGGSGTA"
BASE COUNT         2117 a         4854 c         4483 g         2199 t
ORIGIN      
        1 ctgcttggcc accgactggg cgtagatgaa cccggaacag ccggccgaaa ggtcgaaggc
       61 agcggcccgg ccacacccga gcgcgtgttg caccgtacat gcggtggccg gcagcggcat
      121 gtcgggcgtc acggtggcca agatgatcag gtcgatctgt tccggctcga ctcccgcggc
      181 ctccatcgcg cgacggctgg cctcgacgca gtactgcttc agcgaatcac cggctgcggc
      241 gacgcgtcgt tcgcggatcc cggtgcggct cgtgatccac tcgtccgagg tgtcgaccat
      301 cgtctcgagt tccttgttgg tcaacacgcg ggccggaaca ctcatgcccg taccgatgat
      361 cagggcgctg cgttcggcca tgcacaatct cctaaagacg acgggcgagt gcgagcggtt
      421 cgacgtccgc cgggaggcga cgtttctggc accgcgagat catacctcaa accccaccgg
      481 gcggtaacgg agccggtcgt gggtcacgca catcgtgtcg gcgggatcgc ggctcagatt
      541 tcgggcgggg tgacgacctc gcgccccttg tactgcccac agtggggaca gacccggtgc
      601 ggcagcttca tctcgtgaca gttcgggcaa accgacaggc cgaccttcgg cagcgcgtcg
      661 tgagcacggc gtttgtcccg tcgcgctttc gagtgccttc tctttgggtt cggcattcct
      721 ctacctctgt cccggcggct gctacgtcag tgctgttcga gcttcttctt gagttccagc
      781 agcggagcga gccggggatc ggtctcctcg ctccggcagg cgcactctag tcggtttcgg
      841 ttgaccccgc aagtcgggca gagcccggcg caatcggggc gacagagcga cttgagcgga
      901 acctgcaggt agatctgctc gcggacgatt tcctccagac gcgcctgacc tccttcggcg
      961 tggaacaaga gcgtcgcccg atcgttcgac ccgtcgtcgg acgcacccag ctcgacggcg
     1021 ttcggcacga gaatcaggtg gaactcgacg gcgatcggca gctcgacggc gtcgaggcag
     1081 cggttgcagc tcatccgtac ccgagcctcg agcgcaccgc ggaaatcgac gccgtcggca
     1141 ccgcgcctga ggtcgccgtg aacgtgggcc tgcagcacct ccacctgctc cggtccctcg
     1201 agcgggagcg gtgcgaggtg gatcgacgtg tcgaaccgga tcccctccgg ctccactttg
     1261 tgaatgtcga tgagcatcgt ccatccccgt gcgtggcccg aggattatag cagtcacgga
     1321 cgggccggcc ggtcgcgccg ctcaacgaag cgcggccagt tccttcgtcg cctcttcgtg
     1381 ctcgggcacc agcctgagcg cctcttcgaa ctggcgccgc gcgcgcaggt gcaatccgcg
     1441 cctcttgtag aaccggccga ggctgatcca gtactgagcg ttccacggat cgagctcggt
     1501 cgccttggtg tagttctgct cggccattcg ctgccaacgc gcctccgggt tccgcacctg
     1561 gcaatcggcc accagcgagt agaaccgcgc atcctcggcc gagtaggaaa tcgccagctt
     1621 gccgtactgg atcgcgttgt ggaaatcgcc ggcgagcatc gcctggcgcg ccttgttgta
     1681 gtacagctcc ccgacccgcg ccgcctcgtc ggcttccgtc cggcgcttcg tcttgtccag
     1741 ctcggggcgc atcagcagat cctcggcgct gatgttctgg gtcggcgtac gatcgacttc
     1801 ccgttcgctc cgtttctcgc tcctggccgg ctgcgtcagg ttcagatagg cctcgaccaa
     1861 gcgtgactcg atcacgccga gctccgagcg aaaacgatcg cgtacggacg ggagcagccg
     1921 gtcgcgcgcg aacaacgact tggcctcggc gtaggcgcgg tcgatcgcgg tgcgcgaggc
     1981 cgtcgtcgtc acgccgagaa tctcgtgcgg gttctgcttg cgcattcggc tgtaggcctc
     2041 gctgatcgtc ttctcctgca tccgctcgag cgcacagcgg tcggcgtggt ctcgcaggat
     2101 gttcgccacg cgaaacggtc cgtcggccag tgtcggtcgg aactcggcga caccgaggat
     2161 caacaggccg aacacgaacc gagaagcggc gccttcctcc tccggcggca gcgtggaaat
     2221 catcgcgcga aagtcggttc gaccgtcgat ccgcgacagg acgaagccct gcgtgcgcga
     2281 caacgtcagc ttctcgagcg gaatcggcac gggcttgcgc agggtcagct cgtggtcgag
     2341 cccgcgcatc gcctcgatca cgccgtcgaa atcggcgatc gaggcgaccc cgtgcaggat
     2401 cagacgcagc gtggacagca cgtcgctctc gagcaaatcg acgccccagt cccgttcctg
     2461 gaacgcggcg acccccccgt cccagttgaa cgtggcggcg atgatcttct ccgccagctc
     2521 gcgggccacg gtgacgagcg aggacttggc gaccaggccg cggttgagca acgcttgcgg
     2581 aagctcgacg gggtccttga ccacacgctg ggcctcgagc agcgctcccg cagaaatccg
     2641 ccccgaatcg accaacgctt gcccgaggtc gtgaggcggc tcgttcgagt cgacgtagtc
     2701 gatcaggccg cggttgaaga agacccgagc ctcacgatcc tcgtcggaaa gatgcagctc
     2761 acccgttctt tcgctgatgt agagatcgcg gaagatctcc gccagcgagt gtgagttgaa
     2821 atgggactgc atctcgtacg cgggctcccc tggtcacgcc ggttgccgag tacaatatag
     2881 tggacgcgcg ccgcaaggaa aggcgagggc aacagcgacg gtcccggcgg ccgtccgtgg
     2941 ccgcaacgtg ttgaaaagca tacggttatt cgaccggtcc gcggctcggt cgagcgttca
     3001 gtccgcgacg gccggcgcca cgctggtcaa ccgcaagtcg gcggccgcgg tcgcggcggc
     3061 cggtcggcgg agccgatcgg cctcgtgcag cactttctcc acgtaccgct ccgtctcggg
     3121 atagggcggg atcccgcggt agcggtcgac cgcgttctcg ccggcgttgt aacccgcgag
     3181 cgccagctcg gtgttgccgt cgaaccggtc caacaaccag ctgaggtagc gcgcgccacc
     3241 gtcgacgttc tgcgcaacgt cgaacacgtc cgccacgccg agccgtgccg cggtctcggg
     3301 catcagctgc atcagcccgg tcgcaccctt gggcgagacc gcggcgggat cgaacgccga
     3361 ttcggtgtgc gccacggccg tcagcaggac gggatccaac cggtgctcgg tggccgcacg
     3421 ctcgatatgc ctggcgaaca cacccgctgc gtttcgccat gcctccggcg ggcggttcgc
     3481 ggagccgcgg ggggggagcg cggctcgttc gacgcggctc caaccgtcga cgcgccacgc
     3541 cggaaccgcc agcgctccgc ctccgagcaa ctccagccgg atcgtatcgc cgttccgctc
     3601 gacccgggcc acctcgagta cccggccgtc agcgaagcga accacctcgt cggccgcggc
     3661 cgacgaggcc cagatcgccg aagccagcgc gcacaccgtc accctgcgcg ggattcgcat
     3721 catggactct tttatacgtg cggccgcgac tcccgtcaat cccgacctgc tatcgccgat
     3781 cgaaggctcg ctcgatctcc gcgtgactca ggcgaaaaat cagcgggcgg ccgtgagggc
     3841 aggtcgtggg gttgaccgtt cgaaacaggt cgtcgagcag gcgctgcatc tgctgacgct
     3901 ccagcgtgtg attgaccttg atcgccgcct ggcatgcggc cgacgtgacc agccggtggc
     3961 ggagggacgc ggcggcggag acggtcgatg ggaccgctcg cgcctcgccg agcagctcga
     4021 ggatcagctc tcgggggtcg acggccccgg ccagcgccgg gactcccgtg acgcggatgc
     4081 tgttcccgcc gaacggctcg acgcggaagc cgagccgctc gaactcggcc gtttcgtcgt
     4141 cgaacgtgac acggtccgcc gccgtcagct cgatcgtgat tgggaagatc agccgctgga
     4201 cctcgacgtg gttgtcgacg gcctgggcca ggtagcgttc gaagatgaca cgctcgtggg
     4261 ccgcgtgctg gtcgaccagc agcaggccgt cgtgatcctg ggccaggatg taggatcggc
     4321 ggtattgggc gagcgggacg acggccgagt ccggcgaccc ggtatcgcgc atcgccgggt
     4381 ccgcgaccgg acgcgcggcg gcggcggcga acggcaaccc cgacgtcgcg gggcgtgccc
     4441 ccgccgggct cgccggccca ccgtcggcgg cgggcgatcg gcgatcggcg tagccgctga
     4501 tcgcttccgc gaccgcgcgc ccgcgatcga gcgcggcggg gcgcaggtcg gcgagccgcg
     4561 ggatgaccgc cgcgccggcg agcgccgagc gcaccacgcc ctcgaccagg tcgtgcacgc
     4621 ggaccggctg ccgaaaccgc acctcgacct tctgcgggtg cacgttgacg tcgacgtcgg
     4681 acggggcgca ttcgaggaac acgaagagcg acggatgccg gccgcgcggc atcgtgttgc
     4741 cgtaggcctg gccgacggcg tggagcagca cgcggtcctt gaccgcccgc ccgttgacga
     4801 agaagtgctg gccgtcgcgc cgagtctggg cttcgctcgg gcgacccgcg aagccgtgga
     4861 cccgcagctc gccgtccgac gagtcgaatg gctcgagtcg gcgggcgagc tcgtcgccat
     4921 agagctggcc gattcgctcg cgggcacacg ccgccgggtc gaggtgcagc aactcgcggc
     4981 cggcgtgcac caggcgaaac cggcggtccg gtcgagccaa cgcgtgattc gtcaccagtc
     5041 gtgcgatatg acccagctcg gtcgcttcgc tgcgcaagaa cttgcgtcgg gccggcacat
     5101 tgaagaacag gcgctcgacc cggatcgtcg taccgcgtgg catcgcgatc tcgccgaccg
     5161 accgcacgcg cccgccatgc acctcgacct cggtcccctc accgtcgacg accgcgctct
     5221 gcagagtgaa gcgagaaacc gcggcaatgc tcgacagcgc ttcgccgcgg aagccgagcg
     5281 tcgcgatcga ctcgagacct cgatcgtcga ccagcttgct ggtggcgtgg cgctcgatcg
     5341 ccagcagcgc gtcgtcgcgg tccattcccg caccgtcgtc gatgaccgcg atcatccgcc
     5401 ggccgcctcc cgcacattcg acactgacgg accgggctcc cgcgtcgatg ctgttctcga
     5461 gcagctcctt gacgaccgag gcggggcgct cgatcacctc ccccgccgcg atgcggttga
     5521 tcaacgcatc cgacagcacg cgaatccgac ccacggcgaa ccctcctgcc ggcggaagcg
     5581 taacaccgaa tcagaagact gcgccgatcg cgaggtggaa cacgccgctc gactcgtccg
     5641 cgcgacggtc gagcttccag ccgtattcca tccggatcgg cccgaccggc gtttccagtc
     5701 gcaatccgct gcccagcgta tggcgcaggt cggtcgggtc gaagtcgctc acgcggaagt
     5761 acacgttgcc cgcgtcgtag aacaagacac ccttcaatcg cagtttgcgc agcaacggga
     5821 atcgaaactc ctgattgaac agcagctgtg cctcgccgcc caccggttgg tcggcagcca
     5881 cgccggccgg caacggtgtg ccctcggatg ccgatatctc tccggcggtc gggccgaggc
     5941 gatctcgttc gaacccgcga ttggtcgaat cgcccccggc aaagaaccgt tcggagaccg
     6001 gtacgagatc ggtccgaccg aatggcggcg cgacaccgac gcgaaacgtg gtgacgaagg
     6061 tcgtgccgtt cgaataactc caggtgtggg tctcggtcag cgccgcaagg acgaaatcgg
     6121 cctccgacag gaatggctcg gcgaagaccg cgaagttcac gttgaagtag ttgccgcggg
     6181 tcggcaggat caggttgtcg agcgtcgagc gcagcaatcc gatggcgacg tccccgagga
     6241 tgatgtcctc gacccgctcc tcggccacct ccgccgggtc ggtcacttcg agcagcatga
     6301 cgttctggaa gttgtaccgc aagaacccgc gccagcgctg ggacaggatc cgctcgacgc
     6361 gcaacgcggt cgaacgtcgc tcgatgctga atccgtcctt ttgctcgcgc tcccacagga
     6421 tgttgcccag ccccggcacg gccgcgccga acagacgcgg cgtccgcccg atcagttgga
     6481 cgcgcgaaag gatgctgctg acctcgccct gcacaccgag acttcgcgcc tttccctgca
     6541 cgttgttgtg cgccgaggcg aagctgaatt tgaacttggc ctcggtgttg tagcccacgc
     6601 cggtcgccag cgtcagcggc cgatcttcct cgacgcggac ctcgacgacg tagtacccgg
     6661 gcccgatctc gtgcgcctcg cggtactcga tccggacgtt gcggaacacg cccagacggt
     6721 agagcgactg ttggctgcgc agcagcttgt cacgcgtcac cagctctccc tgcttgatcg
     6781 ccaactcgcg ccggatcagc ctctcgttgg tcagctggtt gccgctgacg cgcacctccg
     6841 ccgcgaccac cttgggcccc ggctcgatct cgaagacgat ctcgacgccg cggtcgctgc
     6901 gctcgaggcg ggccgacacc cgggcgcccg ggtgacccag gcgatcgaac tcgcggcgca
     6961 acgaggcctc gccgccgagc aacgcggatc cggagaacgg ctcgtcgata cggatcccgg
     7021 accacgcttc gaggcgcttg tcgtcgactc ccgtctcgcc ctcgaaacgg atcgcgacca
     7081 cggcctgccg gggaccctcg tggatcgcga tcgagatgtc gatcctccgc ccatcggtcg
     7141 acaggcggag gcgtggcgcg tcgatctcga cgtccaggta gccctggtcg aggtagaggt
     7201 tgcgtatagc cgccaggtcc tcgcgcaacg tcgccggttt gaacacgcca ccgccgaaca
     7261 acgagggcgg gcgcgtcagc atctgcttgc gcacgcgctc ggccgtcatc gcactcgtcc
     7321 cgtgcaacgt gatgccagcg accttgacca actcgccgcg atcgacctcg aagatcactg
     7381 tttctcccgc gccggtgtcg tgcagctcat ggcgcacgtc gaccgcgtag aacccgcgct
     7441 gttgaaggtc ctcacgaatc cgcacgacgg cgtcggtgta gaggtcgtcg gcaaacagcg
     7501 actcggtcca catcgtctcg agctgcgtcg tcagccgacg ctgctccttc ggcttgagac
     7561 cgcggaactc gagctcgatc tgcgggccgg ggtcgacctg atagaccaac tcgaccggct
     7621 gcgacgggtc gtcctccggc gaggtcgtcg cgtcgacccg aaccttgatt cgtccgcgac
     7681 ggacgtagtg gctgcggatg cgctcgacgc cacgaaagac ctccgcgcga ctgtaggccg
     7741 ctccgggctc caccggcagc aagcgcacca gctccgcggc ctccgcctcg tcgaccccga
     7801 cgatcgtcag gcgaccgacc gtccgacccg ccggctccgg ttcggcggcc tgcccgatct
     7861 cggccggtac ggccttgacc gtctcccccc gcggcacgcg ccaccagaag cggtcggtgt
     7921 agaggacgtt gctgccgaca ccgccgttga tgtcgcgctg gaccgtgacc aacatccgag
     7981 gattggcccg ccactccacc tggtacagcc gatcctccgt cgaccgcaga tcggcggaca
     8041 ggatcgccgt cacgtcgtcg ctgaccttct tgcccaccgt gacgcgggtc gtcggatcgc
     8101 cggcctcgat cagcagcgga ttgatcgcca atacgtccag gccgagcgcc tgttgcagct
     8161 ggttctcgaa gcggccggtc aatgcgccgg cgaagtagct cgcggcgagg tccccgctga
     8221 actcggcgtc cgtctgccgg ccgtcgggcg cgagctcctg tagtgtcttg ccggtcgtga
     8281 gtagcgcgac gatgtccggc tgcgacagcg acggcgtgct cgtcaagtcg taacgcaggg
     8341 cgtcgagcgt tccctcgacg cgcagggtga tctcgtagcc gccgatggcg gtccacgcgg
     8401 cgacctggat gtacgggttg atccgctcga tgtcggtgaa gtcgagcgtc ccgctgcgaa
     8461 tgcggtaggt gatgtcccgt acctgtagcg tgccaccttc caccaggccc aggcgcccga
     8521 tgatctcggg gcgccgcggc gtgccgccga tgcgcagatc gaagaagctc tcgagctcgg
     8581 cgacgtcgtt gcgcacccag acgttgccgt cggcgtggac cagcaggtcg agcaccatct
     8641 cggccagcgg cccacccatt tcggtcgggg catattcgcg gctgcctccg ccgaacacac
     8701 cgccgacgcg gaactcctcg ttgtagatgc cgcgcagcat cgtgacgtcg cccgacagcc
     8761 ggggttgctc gagcgtaccc tcgagcacca gctcgccgtc gtacactccg acgaatcccg
     8821 ccggcagcgc gacgcgcacg ttcgagccgc tgacgtcgaa gcgcatcgac tcgaccccgg
     8881 aggcgccgac acgcaacgcc ccgcggccct cgagctctcc tccgccgagc aacgcccgca
     8941 cgcgctcgat cgtcgccgtc tggtcgcgga aagtcgcggt ggcctcgatc tgctcgagcg
     9001 tgtgcggaaa gccgaggtag cgcaggcggc cgccgcgcag cgcgagcgag ccgctgtaag
     9061 tcggcgagcc ggctggtccg tcgatgcgca ggtcgattcg ggcgctgccc gaggcccgca
     9121 cgtcgggcag caccgaggac agcagctcga tgccgaccgt gccgccgatc gaggtcgcga
     9181 tcgacccacc gtcgaagtcg tagcccgatt cgacctccag atccgtcgct tcgccggtca
     9241 gtcgcagcgg tccgatctgc aggcgtccgt cggaaatccg caccggcgcc gagccggcga
     9301 gatcgagcgc gtgcggcccg aaatgcaaac gcgcgtggtc gaacgcaccg tcgatgacga
     9361 gctccggcgc ccgggtgagc ggtccccgga tcgcgaaccg gccgctgagc tcggccgacg
     9421 gagcgggctg agcttcggtc ggcagctcga cgaccgcctg atcgaggcgc gccaccgcct
     9481 cgatcgacga cccggggccc cagccgagcg ccgcctccaa cccccactgt cgagcgtcga
     9541 tcgaggacag ccgtgcctcg acaccggcct gatcgagcgt cagcgttccc gagaagtcgc
     9601 cgagtccaat gcccaaggtg cgcagctcct gcccctcgat ctcgaactgc ccggcgacga
     9661 actgctggcc gacctcgatc gagccgacac cgacgacacg tgcctcgacc gcgccgtcga
     9721 gccccggcaa ccgctcgagt gccacctcga ccgagtcgat gtcgaaccgg gcggtgcggc
     9781 tcgactgatc gagctcgccg cgaccgctga cggtccccgc cggcgaaagc aggtcgaacg
     9841 gctcgagcag aaagcgatcg ccgacgatcc gcaaggtagc ggtgacccgc tcgatcggtt
     9901 ggccgaacgc gttggcctcg acgagcatca gctcgcccga tccgtcgagc ccggcctccc
     9961 cctgcagcgc ggtgacgttg cccgacaacc gcccgccgac gtcgatcgag agatcgagca
    10021 aatccagcag atcggccaac tcgatccgtt cgccgaccaa ccggatctcc tccagtcgaa
    10081 gcggagcgag gccgacgcgg gcgcttccct cgatcgacag gcccggcccg gacagctcgg
    10141 cgtggtcgat cgcgagatcc tcggcgtcga tccgtaacgc gaggtcgagg agatcgtagg
    10201 gccgatcctc gagcattccg tcgcgcagat ccagcccgag cgagaggtcg gtcttggcgc
    10261 cgaagtccac caccgcttcg aacgtgccac ggccgctgat cggcttgcgc acgatctcgg
    10321 gatagtcgat cccgatcgca tcgagtagga gaatcgtggc ggtctgggtc aacgccccgt
    10381 cgtccgtcgt accggaaatc tccagccgtc cgctcggcgg cgtccgctcc agctcggcct
    10441 cgagccgcag cgatgccctc gtaccgatcg tgcgcgccgt cttcgacgcg acggtcagcg
    10501 agccgtcggc gatcgtgacc gtgccgtccg cgtcgaacgg gatctcgccg ggtcccgcgc
    10561 gacggccggt cgccgaaaag gtcgccgtgc cgtcccacga gcggcgatcc tccggccggc
    10621 cggcgagccg cagtcgcgcg tcgacggcgc tggccagcgg cagctcgacg tcgagcagcc
    10681 gagcgagccg ccggccgtcg agggcggacg cctcgagatc gactcgcacg ctggccggat
    10741 cgccgaggac cgcgtcgacc gagccccgca ccgctcctcc gtaggcgcgg gcgtcgatct
    10801 cgcggaaccg cagcgcgcct tgttcgaggt cgagcgcgaa ctcggcccgc tcgatcgcca
    10861 agcgatcgag cgctccgtcg ctgagctgga gacgccctcg gatcttcggt gggtccggac
    10921 cggtgaactc gacgctgccc tcgactcgtc cgtcgattcg tggcagacct gacaacagcc
    10981 cgccgaccgc ggccagatcg gcgtcgatca cgcccgtccc cgtcagcagc ggttgctcgt
    11041 cgagatcgaa ggtggcgtcg gcgtggagcc gaacccccgg tccggtgatc cgcgcgtcga
    11101 tcaactcgat cgtcgtccgc cgccagcgga actgcgacgc caggttcagt tcgacctcac
    11161 gctcgagcgg cggtgtcgcg agcgcggcac gcaaaccgat ctgcccgagc acggagcgtg
    11221 cccctctcgt ccatccgccc tcgatctcga cgtcgtcgac acgcaggtcg agtgggcggt
    11281 cctgctgttc gtgcgtccag cgtccgccga cgatctgcaa gcggtcgacg aagacgtcga
    11341 gctcgaacga tcgagatgcc gtcggcctga ccgacgaacg gtcgagcaaa cggccgtcgg
    11401 tcacgacgct cgggcggacg agcgtcagct cggccaggtg cacgcggcga cgtccgaggc
    11461 ccagcacgta gcgccacggc acggcgagct tgccgtacga gacgctggcc tcgaaccggc
    11521 cggtcgacgg gtcgacgcgc cagacgcgca ggttggtgaa ctccaggctg aacctctgcg
    11581 gccggaggtg gaaccgctcg acctcggcgc gcaggccggc ggcggccagc gaacgtgcga
    11641 ccgccgacca cgcccactcg tcgagctgcc gccaggcgac gaccaacccg cagacgatcg
    11701 ccacgacgac gagcgccgcc ccgccgcgga tccaccagcg cagcctgcct cggcctctcg
    11761 gggaacgtgg tcggtcaggc tcggtaccgg acacttcggc tcaagactct ccgcgtcgtc
    11821 gctctcgcgt cgtctgtccc gagcagggta ccagcaccgc cgggccgcca gcacggtcgg
    11881 tcggtgggcg gcctcacacc gtcagctcgt cctcggtctc ttcgtcaccc gccggatcgg
    11941 tgtcgaccgc gtcgctggga gcttcgtctt ccgagtcgtc ctcgacctcc tggtcgggtg
    12001 cctcttcctc ggagtcgtcg tcgatgatcc gcgagcggga cgacgtctcg gaggaatcct
    12061 catcctcgtc ctcgagctcg aacggtcggt cctgcagcaa gccctgttcc tcgagctcct
    12121 gacaacggat gcaatggcgg gcccaaggct cgacctccag gcgcttctcc gggatctcct
    12181 gagcacactg catgcaactg ccgaactcac cccggtcgat ccgccgcagc gcttcctcga
    12241 ccagccgcat ctgtcgtcgc tccatctcgg tcaacgacaa gctgaagtcg cggtcgtacg
    12301 agctgaccgc gtagtcgatg taatcctcgg tgccgtcggg gaccgaatcg cgcgtattgc
    12361 ccttggtcga gtggtaggcc tcgaccagcg acttgtgcct ggccagcagg acatcgcgga
    12421 agcgcttgag ctgacggcgc cgcaccgccg tcttggcggc ccttcccgcc ttcttgccct
    12481 tggtcggctt ggcgccggcg gccttcttgg tcggcgcctt ggtcgtcacc gctgccccgg
    12541 ccgtcttcgg cttcttggcg gacgccgcct tcctcgaggc cgtcttgcct gcctgggctt
    12601 tcttggtcgt cgcggctctc gtcccgccgg tctttttcgg cttcgtcacg cgcgcggctg
    12661 gtttcggctt gggacgggcc gccgccggtt gctttttcgc tttttgcgcg gcctggccct
    12721 tcttggccgc cgccttcttc ttggagttca cggtcctggc catgcgacct ccgctgcgac
    12781 tcccggcgag ggcgaatccc gaccgccggc gccgcccccc gaaaagggcg cgctactata
    12841 gaattcccgc cgcggagcgt caagctcttt cacgacccgt tcgaccctga tgcgtcacgc
    12901 cgtcccgctg ccacctccct gacccgcggc cagcgccgcg tgcgccccgg ccaaccgcgc
    12961 caccggaacc cggtacggcg agcagctgac gtagtcggcg cccgcctggt ggaagaagat
    13021 caccgagcgt gcgtcgccgc cgtgctcgcc gcagaccccg atcttcagct tcttcgaccg
    13081 gcgcctggcc tcctcgatgc acagccgcac gaggcgcccc acaccgttga cgtccaccgt
    13141 ctggaacggg tcggcgctca gcaccctgtg ttccaggtag aacgggagga acttgccgac
    13201 gtcgtcccgg ctgtagccgt aggtcatctg cgtcagatcg ttggtgccga acgagaagaa
    13261 ctgtgcgtcc gcggcaatgc ggtcggccac caaacacgcg cgcgggatct cgatcatcgt
    13321 gccgacgcgg tacggcagct tgcccccgtt cttgctctgg acttcggcgg cgacgcggtg
    13381 cacccgctcg gcgacgatct gaaactcctc gggcgtcccg accagcggga tcatcacctc
    13441 cgggtggacc tcgatcccct gcttgcggca gatcgccgcc gcctcgaaga tcgcgcgcgc
    13501 ctgcatgtcg tagatcgacg ggaaggtgat tcccagccgg cagccgcggt ggccgagcat
    13561 cgggttcagc tcatgcaggc tgttcaccgt gtcgcgcagg acctgcgcgt cgatgccgac
    13621 ctgctgggcg acgcgctcct ggtcctccgc ggt
//
LOCUS       DASPGN010000166         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_144312, whole genome shotgun sequence.
ACCESSION   DASPGN010000166 DASPGN010000000
VERSION     DASPGN010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144312"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VD788_04725"
     CDS             <1..336
                     /locus_tag="VD788_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75602.1"
                     /translation="HLDKGKGQDDPLNRAILEVLEKVADLEDAGDAEMEEVAIHASAI
                     RNAFFDDRKRMEDVIPPVLLVSVKESDEILPRRTAVVARAGRRTTSLTRGSVRRSAST
                     RKKTTRKQR"
     gene            344..562
                     /locus_tag="VD788_04730"
     CDS             344..562
                     /locus_tag="VD788_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75603.1"
                     /translation="MVRQGGEIVSSRNGSRSSSENGKHKSKRIELNADEVATLLAAIR
                     RYRHTIPVYLASSKPELILLDAVIRKLR"
     gene            658..1698
                     /locus_tag="VD788_04735"
     CDS             658..1698
                     /locus_tag="VD788_04735"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HXV75604.1"
                     /translation="MPVTNSKSTAKAARLVELLRGKRSLLIVMQDNPDPDSIAAAAAL
                     RRIANTLSEVQCSIAYGGVIGRGENRAVAGYLGLNFRPIEEIDFDKYDAVALVDTQPC
                     TGNNSLPADRKADIVIDHHPLRPETRGAPFSDVRKKYGAVSTILVEYLGELGITPDPP
                     LATALLYAIRSDTADLGAEAVQADIDATEALYPLANTRMLSAIQRGSVPRHYFQALAT
                     TLERARVYDHSIVADLDEVENPDMMGEMADLLLRHEGISWVLCHGVHDGKMLLSMRTS
                     QSERRAGDVMHTVVSRLGTGGGHASAAGGQILLKKGTRAERESLGRRLRERFLRETGD
                     SSGRGRKLVGGP"
     gene            1695..2384
                     /locus_tag="VD788_04740"
     CDS             1695..2384
                     /locus_tag="VD788_04740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV75605.1"
                     /translation="MRIVRTRRGARIVGGGAVISEMLARPGATHSVFDVLAAAIAALA
                     PGPRVAVLGFAAGGLLGPLRAMGHRGAIDAVDIDPTGERLFRRSCRAWAGVVRFSRSD
                     AVRWLTHRRRRFDFILEDLSIVERGRTTKPVASFETLPRRMHRALSPGGFALFNLLSV
                     DGMSWSALERAVGRPYRSAVSVRFDEFENRLVLARRGSLESTRTTGRALRGQLRSIDS
                     RLAGRIELRTL"
     gene            2443..>3178
                     /locus_tag="VD788_04745"
     CDS             2443..>3178
                     /locus_tag="VD788_04745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV75606.1"
                     /translation="MTRSDGDPRQPATIPRETERVLGLLRRYDRPGPRYTSYPTAVEF
                     HESFDEASYRAKLAEVNRLPDEPLSLYVHIPFCEERCTYCGCNVVITKKRHVAAGYLD
                     YLQREIDLLAEQLPARRRVSQYHWGGGTPTYLSPQQMEQLHRRIAGHFDIDPDGESAI
                     EIDPRVTSPEQMTLLKELGFNRLSMGVQDFDPEVQAVVNRNQTEDETRRLYDRCRELR
                     FDSINFDLIYGLPRQTMSSFQRTVERV"
BASE COUNT          524 a         1056 c         1115 g          483 t
ORIGIN      
        1 cacctggaca agggcaaggg gcaggacgac ccgctgaacc gcgcgattct cgaggtgctg
       61 gagaaggtcg ccgacctgga ggacgcgggt gatgcagaga tggaagaagt cgcgatccac
      121 gcctcggcga ttcgcaatgc gttcttcgac gaccgcaagc ggatggagga cgtgattccg
      181 ccggtgctgc tcgtctcggt caaggagtcg gacgaaatcc tcccgcggcg gaccgcggtc
      241 gtggcccgcg ccgggcggcg taccacgtcg ctcacgcgcg gcagcgtgcg gcgctcggcg
      301 agcacccgga agaagacgac tcgcaagcag cgctgagcgg gcggtggtcc ggcaaggggg
      361 cgagatcgtg tcgtcgcgca acggtagccg ctcgagctcc gagaacggca agcacaagtc
      421 gaagcggatc gaattgaacg cggacgaggt cgccacgctg ctggcggcca tccgccgcta
      481 ccggcacacg attccggtct atctcgcctc gagcaagccc gagctgatcc tgctcgatgc
      541 ggtcatacgc aagctgcgct gatggccccg cctcaggccg gagtgcgtgc ggcacgtcgt
      601 gatttgacga gacggtacgc cgcggtccag actgttcggc acgatcggag ccgcccgatg
      661 ccagtgacga acagcaagtc gacggccaaa gccgctcgtc tcgtcgagtt gctgcggggc
      721 aagcggtcgc tgttgatcgt gatgcaggac aatcccgacc cggacagcat cgccgccgcc
      781 gcggcgcttc gccggatcgc caacacgttg agcgaggtgc agtgctcgat cgcctacggc
      841 ggcgtgatcg gtcgcggcga gaatcgtgcg gtggccgggt atctcgggct gaatttccgt
      901 ccgatcgagg agatcgactt cgacaagtac gacgccgtgg cgctggtcga cacgcaacca
      961 tgcacgggca acaactcgtt gcccgccgac cgcaaggcgg acatcgtcat cgaccatcac
     1021 ccgctgcggc ccgaaacccg gggcgcgccg ttcagcgacg tacgcaagaa gtacggcgcg
     1081 gtgtcgacga tcctggtcga gtacctgggc gagctcggga tcacacccga cccgccgctg
     1141 gcgaccgcgt tgctctacgc gatccgcagc gacacggccg acctcggcgc cgaggcggtt
     1201 caggccgaca tcgacgcgac cgaagcgctc tatccgctgg cgaacacccg catgttgagc
     1261 gcgatccagc gcgggagcgt cccgcgccac tacttccagg cgctggcgac cacgctcgaa
     1321 cgcgcccgcg tctacgacca tagcatcgtc gcggatctcg acgaggtgga gaatcccgac
     1381 atgatgggcg agatggccga tctgctgctg cggcacgagg ggatctcgtg ggtgctgtgc
     1441 catggggtcc acgacgggaa gatgctgctg tcgatgcgca cctcgcagag cgagcggcgc
     1501 gccggggacg tgatgcacac ggtcgtctcc cgtctgggca ccggcggggg gcacgcctcg
     1561 gcggccggcg ggcagatcct gctcaagaag ggaacccgcg ccgagcggga gtcgctcggc
     1621 aggcgactgc gggaacgctt cctgcgggaa actggggaca gcagcggccg cggacgaaag
     1681 ctggtgggcg gtccgtgagg atcgtccgca cgcggcgagg ggcgcggatc gtcggcggcg
     1741 gagcggtgat cagcgagatg ctggctcgtc ccggggcgac gcactcggtg ttcgacgtgc
     1801 tggccgccgc catcgccgcg ctggcccccg gcccccgcgt cgcggtgctc ggcttcgccg
     1861 cgggcggcct gctcggcccg ctgcgcgcga tgggtcatcg cggtgcgatc gacgcggtgg
     1921 acatcgaccc gacgggggag cggctgtttc gccgctcgtg tcgcgcctgg gccggcgtcg
     1981 tccgattctc acgttcggac gcggtacgct ggctgaccca tcggcggcgg cggttcgact
     2041 tcatcctcga agatctgtcg atcgtcgagc gcggacggac gacgaagccc gtcgcctcct
     2101 tcgagacgct gccgcggcgg atgcaccgag cgttgtcacc cgggggcttc gccctgttca
     2161 acctgctgtc ggtcgacggg atgagctggt ccgcgctcga gcgggccgtc ggccggccgt
     2221 accgctcggc cgtgtcggtt cgcttcgacg agttcgagaa ccgcctcgtc ctcgcccggc
     2281 gtggatcgct cgagtcgacg cgaacgaccg ggcgggcgct gcgcgggcag ttgcgctcga
     2341 tcgactcgcg tctcgccggc cgaatcgaac tccgcacgct gtgagcgatg cttcttgctc
     2401 cccccacgtg agaggctatg atggacccga gggaggagac ggatgacgcg atcggacggg
     2461 gacccacggc agccggcgac catcccgcgg gagaccgagc gcgtgctcgg cctgctcagg
     2521 cgctacgatc gtccggggcc ccgctacacc tcctatccta cggcggtcga gttccacgag
     2581 agcttcgacg aggcgagcta ccgtgcgaag ctggcggagg tcaaccggct acccgacgaa
     2641 ccgctgtcgc tgtacgtcca catcccgttc tgtgaggagc gctgcaccta ctgcggctgc
     2701 aacgtggtga tcacgaagaa acggcacgtc gcggccggct atctcgacta tctccagcgg
     2761 gagatcgacc tgctggcgga gcagctgccc gcccgccggc gggtgagcca gtaccactgg
     2821 ggcgggggca cgccgaccta cctgtcgccg cagcagatgg agcagctgca ccgacgaatc
     2881 gccggccact tcgacatcga ccccgacggg gaatcggcga tcgagatcga tccgcgcgtc
     2941 acctccccgg agcagatgac cctattgaag gagctgggct tcaatcgcct gtcgatgggc
     3001 gtccaggact tcgacccgga ggtccaggcc gtcgtcaacc gcaaccagac cgaggacgaa
     3061 acccgacgct tgtatgaccg gtgtcgcgag ctgcggttcg attcgatcaa cttcgacttg
     3121 atctacggac tgccccgaca gacgatgtcc tcgtttcagc gtaccgtcga gcgcgtga
//
LOCUS       DASPGN010000167         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_378551, whole genome shotgun sequence.
ACCESSION   DASPGN010000167 DASPGN010000000
VERSION     DASPGN010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378551"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VD788_04750"
     CDS             <1..620
                     /locus_tag="VD788_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HXV75607.1"
                     /translation="LIRAAINYAAINGRRSVTLVHKGNIMKFTEGAFRDWGYEVARER
                     FANETVAEGDLTGAAPKDKVVIRDRIADSMFQQILLRPREYSVIATPNLNGDYLSDAA
                     AAQVGGLGMAPGANMSDEIAFFEATHGTAPKYAGQDKVNPGSLILSGRMMLEHLGWDA
                     AATRVDNGLAGAIANKTVTYDLARQMEGATEVKCSEFASAIVDCM"
     gene            625..1548
                     /gene="trxB"
                     /locus_tag="VD788_04755"
     CDS             625..1548
                     /gene="trxB"
                     /locus_tag="VD788_04755"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HXV75608.1"
                     /translation="MNQVIIVGSGPAGLTAAIYTARANLAPLVIEGLAAGGQLMNTTD
                     VENYPGFPDGIQGPEMIELFKRQAERFGARFIAGDATRVDLAGPPFTVWTDDGQHRAR
                     SIILATGASPRTLGIPGEREYAGHGVSYCATCDGFFFRDQEIVVVGGGDTAMEEALFL
                     TRFGRRVTVVHRRDELRASKIMADRALAHAKIEFVWNSVVEEVLGDGRKVGAIRLRDL
                     NSGQTVERPCGGFFVAIGHLPNTALVRAGLAMDEQGYLELEHGSTTKVPGVFAAGDVH
                     DHTYRQAVTAAGAGCRAAIDCERWLERQTTG"
     gene            complement(1552..>2302)
                     /locus_tag="VD788_04760"
     CDS             complement(1552..>2302)
                     /locus_tag="VD788_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXV75609.1"
                     /translation="LLIVHGGPESHARNGWVTNYSRPGQIAAARGYAVYYPNYRGSTG
                     RGVAFSKLGQGDAAGAEFDDLIDAIDALARDGLADAGRVGVTGGSYGGYATAWCATRF
                     TDRFRAAVMFVGISNKLSKGLTTEIPVEDRTVHTLFDPWSRWQYSLERSPLYHAEQAR
                     TPLLIAGGTADTRVHPSQSLQLYRALKLIGRTPVRYVRYPGEGHGNLNAAARDDYGRR
                     LMRWIDHFVLGDAREPPPYELDSPVPAPPAD"
BASE COUNT          369 a          787 c          799 g          347 t
ORIGIN      
        1 gcctcatccg ggccgcgatc aactacgccg ccatcaatgg ccgccgctcc gtcaccctcg
       61 tccacaaggg caatatcatg aagttcaccg agggcgcctt tcgcgactgg ggctacgaag
      121 tcgcccggga acgcttcgcg aacgagacgg tcgccgaggg cgatctcacg ggcgcggcgc
      181 cgaaagacaa ggtcgtgatt cgcgatcgca tcgccgactc gatgttccag cagatcctgc
      241 tgcggccgcg cgagtactcg gtgatcgcca cgcccaacct caacggggac tacctgtccg
      301 acgcggcggc ggcacaggtc ggcggtctcg gaatggcgcc cggggcgaac atgtccgacg
      361 aaatcgcgtt cttcgaggcg acgcacggca ccgcacccaa gtacgccggt caagacaagg
      421 tcaaccccgg gtcgttgatc ctctccggcc ggatgatgct cgagcacctc ggctgggacg
      481 cggcggcgac gcgcgtcgac aacgggctgg ccggcgcgat cgcgaacaag acggtgacct
      541 acgatctggc gcgacagatg gaaggcgcga ccgaagtcaa atgctccgag ttcgcctccg
      601 ccatcgtcga ctgcatgtga cgcgatgaac caggtgatca tcgtcgggtc gggcccggcg
      661 ggcctgacgg ccgcgatcta caccgcccgc gccaatctcg ccccgctggt catcgaaggg
      721 ctcgccgccg gcggccagtt gatgaacacg acggacgtcg agaactaccc ggggttcccc
      781 gacggcatcc aagggcccga aatgatcgag ttgttcaagc ggcaggccga gcgcttcggc
      841 gcccgcttca tcgccggtga cgcgacgcgc gtcgatctgg ccgggccgcc gttcaccgtc
      901 tggaccgacg acggacagca ccgcgcgcgc tcgatcatcc tggccaccgg cgcctcgccg
      961 cgcacgctcg gtattccggg cgagcgagaa tacgccggcc acggcgtgtc gtactgcgcg
     1021 acctgcgacg gcttcttctt ccgcgaccag gagatcgtcg tcgtcggtgg gggcgatacg
     1081 gcgatggagg aggcgctgtt cctgacgcgg ttcggccgac gggtgaccgt cgtccaccgg
     1141 cgcgacgagc tgcgcgcgtc gaagatcatg gccgaccgtg cgttggctca cgccaagatc
     1201 gagttcgtct ggaactccgt cgtcgaggag gtgctgggtg acgggcgaaa agtcggggcg
     1261 atccgacttc gcgatctgaa cagcggtcag acggtcgagc gtccatgcgg tggcttcttc
     1321 gtcgcgatcg gccacctgcc caacaccgcg ctggtccgcg cggggctggc gatggacgag
     1381 cagggctacc tcgagctcga acacggctcg acgacgaagg tccccggcgt gttcgccgcg
     1441 ggcgacgtcc acgaccacac ctaccggcag gcggtcaccg cggccggcgc cggttgccgc
     1501 gcggcgatcg actgcgagcg ctggctcgag cgacagacga ccggctgacg gtcagtcggc
     1561 cggcggcgcg gggaccgggg agtcgagctc gtacggcggg ggttcgcgcg catcgcccag
     1621 gacgaagtga tcgatccagc gcatcagccg ccggccgtag tcgtcccggg cggcggcgtt
     1681 gaggtttccg tgcccttcac ccgggtagcg aacgtaacgc accggcgtcc ggccgatcag
     1741 cttcagcgcg cgatacagct gcaggctctg cgacgggtgg acccgcgtgt ccgcggttcc
     1801 gcccgcgatc aacagcggag tgcgcgcctg ctccgcgtgg tacagcgggc tccgctcgag
     1861 gctgtactgc cagcgcgacc agggatcgaa caaggtgtgg accgtccggt cttcgacggg
     1921 gatctcggtc gtcagaccct tgctgagctt gttgctgatg ccgacgaaca tgaccgcggc
     1981 acggaaccgg tcggtgaacc gcgtcgcgca ccaggccgtg gcgtagcctc cgtacgaccc
     2041 gccggtcacg ccgacccgtc cggcgtcggc cagcccgtcc cgggccaacg cgtcgatcgc
     2101 gtcgatcaag tcgtcgaact cggcgccggc ggcatcgccc tggcccagct tggagaaggc
     2161 gacgccgcgt cccgtgctgc cgcggtagtt cgggtagtag acggcgtatc cgcgcgccgc
     2221 cgcgatctgc ccgggtcgtg agtagttggt gacccagccg ttacgcgcgt gggactcggg
     2281 tcctccgtgg acgatcaaca ac
//
LOCUS       DASPGN010000168         8143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1198580, whole genome shotgun sequence.
ACCESSION   DASPGN010000168 DASPGN010000000
VERSION     DASPGN010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8143
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198580"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /gene="boxC"
                     /locus_tag="VD788_04765"
     CDS             complement(<1..1570)
                     /gene="boxC"
                     /locus_tag="VD788_04765"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HXV75610.1"
                     /translation="MKTDTRASGPILFESRPDRYRHWKLAVDGELATLTMGVEESGGL
                     RDGYELKLNSYDLGVDIELQDAVQRLRFEHPRVRAVIVTGGLDRVFCAGANIHMLASS
                     SHAFKVNFCKFTNETRLSIEDASVHSGQRYLAALNGTCAGGGYELALACDRIVLVDDG
                     NSAVSLPETPLLGVLPGTGGLTRLVDKRKVRRDLADVFCTVAEGVKGKRALEWGLVDG
                     VVAKSRFRDEVLERARELAAGSATRAERGVELAPLDKAIEDDRVVYSTVLVEVDRARR
                     SATLTVRGPDGGEPTDAASIRKRGSRFWPLCAFRELDDALCHLRVNEPEIGLLLLETT
                     GEIGPMLELDETIDRLRDDWFVNEVRLLIARTLRRLDLTAKSIFALIEPGSCFAGTLF
                     ELALAADRSYMLDDPEQPVQVAISPMNADRYPMSHGATRLAARFLGHPERAASLAATP
                     QRYDAERALAAGLVTVVADDIDYEDEVRLAIEERASLSPDALTGMEASLRFAGPETCD
                     SKIFGRLSAWQNWIF"
     gene            1647..2384
                     /locus_tag="VD788_04770"
     CDS             1647..2384
                     /locus_tag="VD788_04770"
                     /inference="COORDINATES: protein motif:HMM:NF006015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXV75611.1"
                     /translation="MTLLDHLARRVRGLRENRGWTRGELATRSGLSVRFLARVEGADG
                     NISVLRLEALARALDTTPDRLVRPAAGPRRIVTLVGLRGAGKSAVGPLLAARLGCPFV
                     EMDALITESSGLTLDQLFELHGERYYRRLEHDTVRGILAREDAAVVAAAGGVVNEPRT
                     WKLLRERTTVVWLRASPEDHWARVVAQGDRRPMADHPAAMEELRSILAAREPIYRDAA
                     ITVNTSGASPEQIRATIERELVALANE"
     gene            2464..3312
                     /gene="amrB"
                     /locus_tag="VD788_04775"
     CDS             2464..3312
                     /gene="amrB"
                     /locus_tag="VD788_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HXV75612.1"
                     /translation="MLGPTVAGSWYPSNPAELRRQIEGFLSAGGATEKAKGSPVALIA
                     PHAGFVYSGAVAARAFRLLRARPIRRVVLIGPSHYAAFRGARVPRATTYRTPLGEIDL
                     DATAIESVLDPPFVAADDEPFEREHCLEAEIPFLQCVLADGWSMVPMLIGGATTAERR
                     SELSRALQPLIDGGTVVVVSSDFTHYGDGFHYTPFRRDVRDRIRELDMGAIDRIVAID
                     RTGFDSYVAETGATICGRVAIGVLLGLEFGAVDARLIDYATSGEITGDWGHCVSYAAV
                     GFWGGA"
     gene            3309..3908
                     /gene="amrA"
                     /locus_tag="VD788_04780"
     CDS             3309..3908
                     /gene="amrA"
                     /locus_tag="VD788_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HXV75613.1"
                     /translation="MTADSAPLRDDERRALLRLARTAIEDRLLADHRLDRAIAEVDVT
                     PTLARPAGLFVTITDTRDRARPLRGCVGTMRGSKPLYLEVAETARAAAFDDPRFPPLA
                     CDELPRVRLAISILSALRPAPDWRAIRPGIDGVQLVREAKRAVFLPQIAVAQRWDREQ
                     LLTQLALKAGLAADGWRSAELFVFRTDSFEEPDAAHSGG"
     gene            complement(3893..4564)
                     /locus_tag="VD788_04785"
     CDS             complement(3893..4564)
                     /locus_tag="VD788_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXV75614.1"
                     /translation="MKAIILAVALAGAVGMAQGDDPAGTGHDATVHHDFKNAKEWAEK
                     FDSPDRAEWQKSRIVMRVLGVAEGETVADIGAGTGYFTQILSRALGPTGKVLAVDIEP
                     EMLAYIEQRDDLGTTPVETILARADDPLLGENRVDLALLVNTWHHIDDRLSYLERLRV
                     ALKAGGRVAVIDWHEGELPIGPPVGSKLSRAEVVAEFAERNWRLESESVALPYQYLLV
                     FYPPE"
     gene            4691..5587
                     /gene="cyoE"
                     /locus_tag="VD788_04790"
     CDS             4691..5587
                     /gene="cyoE"
                     /locus_tag="VD788_04790"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HXV75615.1"
                     /translation="MAQYVDAEASRAPRLARFADFAALGKPRLSVMVIFTTAIGVWLA
                     PAPPLLATTLLFLLATSCLVAAANTFNCWIERDSDGLMHRTCRRPLPAGRLEPRAALY
                     YGIVLSIASLTAIGLTSNGLTTALGALALAVYVLVYTPMKRVTPWAVVVGAVPGALPP
                     LMGWTAASGEVSIPGLFLFGVLFLWQLPHFIAISLYLRDDFARAGIRALPVAIGRRAT
                     RPYLIASLSALVLFSLAALPLGVAGVGYTVVAAVIGVVWLVRACTGDDARAAGDGWAR
                     RMFGLSLLYLPLLVSALVLDAF"
     gene            5704..7605
                     /locus_tag="VD788_04795"
     CDS             5704..7605
                     /locus_tag="VD788_04795"
                     /inference="COORDINATES: protein motif:HMM:NF015515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HXV75616.1"
                     /translation="MLLAVATAAAAAPACGAVEVEFTVAPPPVAVPDLPARFSIRLSP
                     SSGFASLQVEIRAAETDRLLVTGEAAAGVETVMTIDHATASGRYRVVAAGHPQTTKAI
                     DVRTIPGWSTLAPPVTAILLALLFRQVVPALCAGIWIGGWIGFGGPVVGAMRMIDRHV
                     VGSLATTDHASIIIFSLLLGGMVGIVSRSGGTHGLVDVLRPYATNSRRGQCVTWVLGL
                     LIFFDDYANTLLVGNTMRPVTDRLRISREKLAYIVDSTAAPVTGIALVSTWIGYEVSL
                     IADSLRVTGIDEQAYGVFLRSLPYNFYPILTLLFGMLVATQLRDFGPMRDAERRARAG
                     RVLSDKAVPLSDFDGAALSPVEHKPRRWINAVAPVVVVLVVTFAALWFTGRTSLAGDG
                     TTAESNLRGLATVFGAGDSFEALLWASLSGCIVALLLAVTQRILTVGEAMAAWLNGLK
                     SMTMAIVILVLAWSISDVCVDLGTSGYMVAALSDRLDPRVVPLIVFVISAITAFSTGT
                     SWGTMGILIPLAVPTAYGVAGAAGYDADHATAILLCSISAVLAGAIFGDHCSPISDTT
                     VLSSMASGCDHIDHVRTQLPYAVVVALVAIVCGYLPAGFGLSPWICVLAGSALLFVVL
                     RVVGRSDTG"
     gene            7691..>8143
                     /locus_tag="VD788_04800"
     CDS             7691..>8143
                     /locus_tag="VD788_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HXV75617.1"
                     /translation="MGQTIVEKLAQAHLVDGPRDRPLRAGDFVTVRPRHVMTHDNTAA
                     VMTKFARFGATRVADPRQPVIALDHDIQNRSAANLAKYRSIESFAREQRIDFHPAGSG
                     IGHQIMVERGYVVPGSLVVASDSHANTYGALGALGTPVVRTDAAAIWAT"
BASE COUNT         1099 a         2859 c         2752 g         1433 t
ORIGIN      
        1 ggaagatcca gttctgccag gccgaaagcc gcccgaaaat cttgctgtcg caggtctcgg
       61 ggccggcgaa gcgcaggctc gcctccatcc ccgtcagggc gtcgggggac aggctcgcgc
      121 gctcctcgat cgccaggcgg acctcgtcct cgtagtcgat gtcgtcggct acgaccgtga
      181 ccaagcctgc cgctagcgcg cgctcggcgt cgtaccgttg cggcgtggcg gcgagcgacg
      241 ccgcgcgttc cgggtggccg aggaagcgcg ccgccagcct ggtcgcgccg tgcgacatcg
      301 ggtagcggtc ggcgttcatc gggctgatgg cgacctggac cggctgttcc gggtcgtcga
      361 gcatgtacga gcggtcggcg gcgagcgcga gctcgaacag cgtcccggcg aagcaggagc
      421 cgggctcgat cagcgcgaag atcgacttcg cggtgaggtc gagacgtctc agcgtacgcg
      481 cgatcaacag ccgaacctcg ttgacgaacc agtcgtcgcg caggcggtcg atcgtctcgt
      541 cgagctcgag catgggaccg atctctcccg tcgtttcgag cagcagcagt ccgatctccg
      601 gttcgttgac gcgcaggtgg cacagcgcgt cgtcgagctc gcgaaaggcg cacagcggcc
      661 agaagcgaga gccgcgcttg cggatcgacg ccgcgtcggt cggttccccg ccgtccggtc
      721 cgcgcaccgt gagagtcgcc gaacggcgag cgcgatcgac ctcgaccaag accgtcgaat
      781 agacgacgcg gtcgtcctcg atcgccttgt cgagcggtgc cagctcgacg ccgcgctcgg
      841 cacgcgtggc ggagccggcg gccagctccc tcgcccgctc gagcacctcg tcccggaacc
      901 ggctcttcgc gaccacgccg tcgacgagtc cccactcgag cgcccgcttg cccttgaccc
      961 cttcggcgac cgtacaaaac acgtccgcca ggtcgcgccg cacttttctc ttgtcgacga
     1021 ggcgcgtcag cccgcccgtt ccgggaagaa cgccgagcaa cggtgtttcg ggtagactca
     1081 ccgcggagtt cccgtcgtcg acgagcacga tccggtcgca cgccagcgcc agctcgtaac
     1141 cgccgccggc gcaggtgccg ttgagcgcgg cgagatagcg ctggccgctg tgaacggagg
     1201 cgtcctcgat cgacagccgg gtctcgttcg tgaacttgca gaagttgacc ttgaacgcgt
     1261 gcgacgagct ggcgagcatg tggatgttgg cgcccgcgca aaaaacgcgg tcaagaccgc
     1321 cggtgacgat caccgcgcgg acccgcggat gctcgaagcg gagccgttgg acggcatcct
     1381 ggagctcgat gtcgacgccg aggtcgtagg agttgagctt cagctcgtag ccgtcgcgca
     1441 ggccgcccga ctcctcgacg cccatcgtca gcgtcgccag ctcgccgtcg accgccagtt
     1501 tccagtgccg gtagcggtcg ggacggctct cgaacaggat gggcccgctg gcccgtgtgt
     1561 ctgttttcac ggcgcctcct tcggcgcgaa ttatactgca ctaatggacc gttgcaatcc
     1621 agtatggagg gcactatagt gcacacgtga ccttgctcga ccacctcgcc cgtcgcgtgc
     1681 gcggcctgcg cgagaaccga gggtggacgc gcggcgagct cgcgacgcga tcggggctgt
     1741 ccgtgcgttt cctcgcgcgc gtcgagggcg cggacggcaa catctcggtc ctgcggctcg
     1801 aggcgctcgc gcgggcgctc gacacgacgc cggaccgcct cgtccgcccc gccgccggcc
     1861 cgcgccggat cgtgacgctc gtcggtctgc gcggtgcggg gaaaagcgcg gtcggccccc
     1921 tcctcgccgc ccgcttgggt tgtccgttcg tcgagatgga cgcgctgatc accgaatcgt
     1981 ccggcctgac gctcgaccaa ctgttcgagc tacacgggga gcgttactac cggcggctcg
     2041 aacacgacac ggtgcggggc atcctcgcac gggaggacgc cgccgtcgtc gccgcggccg
     2101 gtggtgtcgt caacgagccg cggacctgga aactgctgcg cgagcgcacg accgtagtct
     2161 ggcttcgggc cagcccggag gaccactggg cacgggtcgt cgctcagggc gaccggcgcc
     2221 cgatggccga ccaccccgcg gcgatggagg agttgcgctc gatcctggcg gcacgcgagc
     2281 cgatctaccg cgacgccgcg atcaccgtca acacctccgg tgccagcccg gaacagattc
     2341 gcgcgacgat cgagcgcgag ctggtcgcgc tggcgaacga atagcctgca cctgtttctt
     2401 ttgaccgagg tgcgctaacc ttggcttgaa atggtctccg atgccgaacg atcgaggcca
     2461 cgggtgctcg gaccgacggt cgcgggcagc tggtatccat cgaaccccgc cgaactccgt
     2521 cgccagatcg agggcttcct gtcggccggt ggcgcgaccg agaaggcgaa gggttcgcca
     2581 gtcgcgctga tcgcgccgca cgcggggttc gtctactcgg gcgccgtcgc cgcgcgtgcg
     2641 ttccgcctgc tgagggcccg accgatccgg cgcgtggtgc tgatcgggcc gagccactac
     2701 gccgcctttc gcggtgcgcg cgtgcctcgt gcgacgacct atcgcacgcc gctcggcgag
     2761 attgacctcg acgcgaccgc catcgagtcc gtgctcgatc ccccgttcgt tgcggccgac
     2821 gacgaaccgt tcgagcgcga gcactgcctc gaggcggaga tcccgttcct gcagtgcgtg
     2881 ctcgccgacg gctggagcat ggtaccgatg ttgatcggcg gggcgacgac cgccgagaga
     2941 cgctcggaac tgtcccgagc ccttcagccg ctgatcgacg gtggtaccgt cgtcgtcgtg
     3001 tcgagcgact tcacgcacta cggtgacggc ttccactata cgccgttccg gcgcgacgtg
     3061 cgtgaccgga tccgcgagct ggacatgggc gcgatcgatc gcatcgtcgc gatcgaccgg
     3121 accggcttcg actcctacgt ggccgagacg ggcgcgacga tctgtggacg cgtcgcgatc
     3181 ggcgtgttgc tcgggctcga gttcggtgcc gtcgacgcgc ggttgatcga ctacgcgacg
     3241 tcgggcgaga tcacgggaga ctggggtcat tgcgtctcct acgccgcggt cggtttttgg
     3301 ggaggtgcgt gacggccgat tccgcgccgc ttcgagacga cgagcggcgc gcgctgttgc
     3361 ggctggcacg cacggcgatc gaggaccggt tgctcgccga tcaccggctc gaccgggcga
     3421 tcgccgaagt cgacgtcacg ccgacgctgg ctcggccggc cggcttgttc gtcacgatca
     3481 ccgacactcg cgaccgagcc cgtccgctac gcggttgcgt cgggacgatg cgcgggtcga
     3541 agccgctcta cctcgaagtg gccgagacgg ctcgcgcggc tgccttcgac gacccgcgct
     3601 tcccgccgct cgcctgcgac gagctcccac gcgtccggct ggccatctcg atcctgtcgg
     3661 ccctgaggcc cgcccctgac tggcgcgcga tccgtcccgg gatcgatggc gtgcagctgg
     3721 tgcgcgaggc gaagcgcgcg gtcttcctac cgcagatcgc cgtggcccag cgctgggacc
     3781 gcgaacagct gctcacgcag ctcgcgctca aggccggtct cgccgccgac ggctggcgaa
     3841 gcgccgagct gttcgtcttc cgcaccgact cgttcgaaga acccgacgcc gctcactcgg
     3901 gcggatagaa cacgagtaga tactggtacg gcagcgccac gctttccgac tcgagacgcc
     3961 agttccgctc ggcgaactcg gctacgacct cggcccgact gagcttcgaa ccgaccggtg
     4021 gcccgatcgg caactcgccc tcgtgccagt cgatgactgc gacgcggccg cccgccttca
     4081 atgcgacccg caggcgttcg aggtagctca accggtcgtc gatgtgatgc caggtgttga
     4141 ccaagagcgc gagatcgacg cggttctcgc ccagcagcgg atcgtccgcc cgcgccagga
     4201 tcgtctcgac gggcgtggta cccaggtcgt cgcgttgctc gatgtaggcc agcatctccg
     4261 gctcgatgtc gaccgccagc acctttccgg tcgggccaag cgcccgggag aggatctgcg
     4321 tgaagtagcc ggtgccagcg ccgatatcgg ccacggtctc gccctccgcg acgccgagca
     4381 cccgcatgac gatccgcgac ttctgccatt cggcacggtc gggtgagtcg aatttttccg
     4441 cccattcctt ggcgttcttg aagtcgtgat ggaccgtggc gtcgtgcccc gtcccggccg
     4501 gatcgtcacc ttgtgccata cccaccgccc ctgccagggc caccgcgagg atgatcgctt
     4561 tcatctggcg ctccttgttc cgagccagta gtattctgcg tctcgtcgcc ggcttcaacc
     4621 tcgcggctgc ttttccacgg ggcgggtcgc gatccgcgag ccgggctggc ggcacagcgc
     4681 gagggatcgg atggcccagt acgtcgacgc cgaggcgagc cgcgccccgc gtctcgctcg
     4741 attcgcggac ttcgccgcgc tcggaaagcc ccggttgtcg gtgatggtga ttttcacgac
     4801 ggcgatcggc gtgtggctcg cgccggcccc gcctctgctc gcgaccaccc tcctcttcct
     4861 gctcgccacg tcgtgtctcg tcgcggccgc caatacgttc aactgttgga tcgagcgcga
     4921 ctcggacggc ctgatgcacc gtacgtgtcg ccggccgctg cccgccgggc gactcgagcc
     4981 gcgtgcggcg ctgtactacg ggatcgtgct gtcgattgcc tcgctgaccg cgatcgggct
     5041 gacctcgaac gggttgacca ccgcactcgg ggcgctggcg ctcgcggtct acgtgctcgt
     5101 ctacacgccg atgaagcgcg tcacgccctg ggccgtcgtc gtcggcgcgg tgcccggcgc
     5161 actcccgccg ctgatggggt ggaccgcggc cagcggagag gtttcgattc ccggtttgtt
     5221 cctgttcggt gtcctgttcc tctggcagct cccgcacttc atcgcgatat cgctctatct
     5281 ccgcgacgac ttcgcgcgcg ccgggatccg tgcgctcccg gtcgcgatcg ggcgtcgtgc
     5341 cacgcgcccg tacttgatcg cctcgttgtc ggcgctggtg ctgttcagcc tggccgccct
     5401 cccgctcggc gtcgccggcg tcggctacac cgtcgtcgcc gccgtgatcg gcgtggtctg
     5461 gctcgttcgc gcgtgcaccg gcgacgacgc ccgcgcggcc ggggacggct gggcgcgccg
     5521 gatgttcggt ctatcgctgc tttacctccc gctgttggtc tccgcgctgg tgctcgacgc
     5581 gttctgacgt cggccgcgtc caccgcgggc cgtgttagga tgggcacccg tcgtccgccg
     5641 agcctccggg tgcgtgtgaa tcgtccaccg cgaatcgaac gatcatggcg cgcggcgact
     5701 ttcgtgttgc tcgccgtcgc cacggcggcc gcggcagcgc cggcgtgcgg cgcggtcgag
     5761 gtcgagttca ccgtcgcccc cccgcccgtt gcggtccccg atctcccggc ccgcttttcg
     5821 atccgcctgt cgccctcgtc cggtttcgcc tccctgcagg tcgagatccg cgcggcggag
     5881 accgatcgct tgttggtcac cggcgaggcg gccgccgggg tggagacggt gatgacgatc
     5941 gatcacgcga ccgccagcgg acgttaccgg gtcgtcgccg ccggtcaccc gcagacgacg
     6001 aaagcgatcg acgtgcgaac gattcccggt tggtcgacgc tggctccgcc cgtcaccgcg
     6061 atcctgctcg cgctcctctt ccgccaggtc gtgccggcgt tgtgcgccgg aatctggatc
     6121 ggcggctgga tcggcttcgg tggaccggtc gtcggggcga tgcggatgat cgatcgccat
     6181 gtggtcggct cgctggcgac gaccgaccat gcctcgatca tcatcttctc gctgttgctc
     6241 ggcggcatgg tcgggatcgt ctcccgctcg ggcgggacgc acgggctggt cgacgtcttg
     6301 cggccgtacg cgaccaactc gcgacgcggc caatgcgtca cgtgggtgct cggcctgctc
     6361 atcttcttcg acgactacgc caacacgctg ctggtcggca acacgatgcg accggttacc
     6421 gatcgcctgc ggatctcccg cgagaaactc gcttatatcg tcgactcgac ggcggcgccg
     6481 gtcacgggca tcgcgctcgt ctcgacctgg atcggctacg aagtgtcgct gattgccgac
     6541 tcgctgcggg tcaccggcat cgacgagcag gcgtacggcg tcttcttgcg ctcgctcccg
     6601 tacaacttct atccgatcct gaccctgctg ttcggcatgc tcgtcgcgac tcagctgcgc
     6661 gacttcgggc cgatgcgcga cgccgagcgg cgtgcgcggg cgggccgcgt gttatcggac
     6721 aaggcggtgc cgttgtccga cttcgacggc gccgcattga gtcccgtcga gcacaagccg
     6781 cgacggtgga tcaacgccgt cgcgccggtt gtcgtcgtgc tggtcgtcac gttcgcggcg
     6841 ttgtggttca ccggccgtac ctcgctcgcc ggcgatggca cgaccgccga gtcgaacctg
     6901 cgcgggctcg ccacggtgtt cggcgccggc gactcgttcg aggcgctgct gtgggcctcg
     6961 ctgtcgggat gcatcgtcgc cttgctgctg gccgtcaccc aacgcatcct cacggtcggt
     7021 gaggcgatgg ccgcctggct caacgggctc aaaagcatga cgatggcgat cgtgatcctc
     7081 gtcttggcct ggtcgatctc cgacgtctgc gtcgatctcg gcacttcggg ctatatggtg
     7141 gcggcgctgt cggacaggct cgatccgcga gtcgtaccgc tgatcgtgtt cgtcatcagc
     7201 gcgatcaccg cgttctcgac gggtacctcg tggggcacga tgggaatcct gatcccgctg
     7261 gcggtgccga ccgcctacgg cgtcgccggt gcggcggggt acgacgccga ccacgccacc
     7321 gcgatcctgc tctgctcgat ctcggccgtc ctcgccggag cgatcttcgg cgatcattgc
     7381 agcccgatct cggacacgac cgtgctgtcg tcgatggcgt cgggttgcga ccacatcgac
     7441 cacgtccgga cgcagctgcc ctacgccgtc gtcgtcgccc tggtggcgat cgtgtgcggc
     7501 tatctgccgg cgggcttcgg tttgtcgccg tggatctgcg ttctcgcggg ctcggcgctg
     7561 ctgttcgtcg tcttgcgtgt ggtcgggcga tcggacaccg gctgagctgg acggtcgcgg
     7621 ccccctcggc tacgctgctc gacgcttcgt gagatcgttc ggacacgtcg tcggcgtgcg
     7681 aggacgcgcg ttgggacaga cgatcgtcga aaaactggcc caggctcacc tggtcgacgg
     7741 accgcgcgac cgaccactgc gggccggtga cttcgtcacc gttcgtccgc ggcacgtgat
     7801 gacccacgac aacaccgccg cggtgatgac caagttcgcc cgcttcggcg cgacgcgcgt
     7861 cgccgacccg cgccagccgg tgatcgcgct cgatcacgac atccagaacc gctcggccgc
     7921 caacctcgcc aagtaccgct cgatcgaatc gttcgcgcgc gagcaacgca tcgatttcca
     7981 tccggccgga agcggcatcg ggcaccagat catggtcgag cggggctacg tcgttccggg
     8041 gagcctggtc gtcgcctccg actcgcacgc caatacgtac ggcgcgctcg gcgccctcgg
     8101 caccccggtc gtgcgtaccg acgcggcggc gatctgggcc acc
//
LOCUS       DASPGN010000169         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_847823, whole genome shotgun sequence.
ACCESSION   DASPGN010000169 DASPGN010000000
VERSION     DASPGN010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847823"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /gene="aroA"
                     /locus_tag="VD788_04805"
     CDS             <1..982
                     /gene="aroA"
                     /locus_tag="VD788_04805"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HXV75618.1"
                     /translation="RFLLAMAALGARSSRLDGDARLRERPLGELAEALREIGARCETA
                     DGGRLPARAGGSTVRGGAVRVAGRRSSQFASALLLIGSTLADGLRLAIEPPVVSSPYL
                     RMTIDTLVSFGGRVDRSGDRSFRVVPGPLGGREVRIEGDHSSASYFLAAAAVVGGRVR
                     VCGLTPASHQPDAALGGMLAAAGCLVRRGADWIEVEHHGPLRPLDLDMADSPDLVPTV
                     AVAASFAGGTSVLRNIAHLRHKESDRLTTIATNLARLGCPAEIGDDSLTIASRGRPTR
                     GALIETASDHRIAMAFALAGLAVEGVAVDDARCVAKSNPRFWQQLAWLTA"
     gene            complement(990..2276)
                     /locus_tag="VD788_04810"
     CDS             complement(990..2276)
                     /locus_tag="VD788_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75619.1"
                     /translation="MRLKRFLASVSPLLLLLAVSAVAAQDDAPPPEAPPQTEEDETPP
                     PPTDAPEPQAAPTEQATTDYFGLYVEAGSGAWSLDDIDSSIQTLSTALSVNTVSFDDH
                     QYGRAAIGWRLKGGKGDFRLIFNGWSEDGYRFDAEGYLAALDPDLGVATEDVRDPLLW
                     WTITVDDGQLHSVNTPPVWTDAPPPAGDDANGNGFVDAGEQRYLTPAFDFTAETFTSL
                     QNRAQTYDFVFGNVWGPRGFQGRWFAGLRYFVYEGNMSAAAWLHGSSSEGVGYTDGAF
                     LRLLNFHQEASGLGPTGLMEARFNFFNQRLQLYLNGQISFIVEDVSTTSGEFLTLVGN
                     TAGEDVTVPAELSLSQTKSTWHTAAEAGARLRLTSGLELQLSYGVVGLLDAVLLPNDI
                     RIPENATEARVQGTSALYNTQDYVLTGWRAGLAFQF"
     gene            2422..>2748
                     /locus_tag="VD788_04815"
     CDS             2422..>2748
                     /locus_tag="VD788_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75620.1"
                     /translation="MCAETKRRTLLLLASCLCAAATPVAGQSTGESLPDRELERLLEL
                     HHTERQLVRLVQVPAVVTTRAGKVVRGLDASDFVVYEDHVPQAIRHVTTEHEEPVSIA
                     FLLDVSG"
BASE COUNT          404 a          920 c          978 g          446 t
ORIGIN      
        1 gcgtttcctg ctcgcgatgg ccgcgctcgg cgcacggtcg tcacgactcg acggggacgc
       61 gcggctgcgc gaacgaccgc tcggcgaatt ggccgaggcg ctgcgcgaga tcggcgcgcg
      121 atgcgaaacc gcggacggcg ggcgcctgcc ggcgcgcgcc gggggctcga ccgttcgcgg
      181 tggcgcggtg cgcgtcgccg gccgccgcag cagccagttc gccagcgcgt tgttgctgat
      241 cggatcgaca ctcgcggacg gattgcgact tgcgatcgag ccgccggtcg tctcgtcgcc
      301 ctacctacgg atgacgatcg acacgctcgt ctcgttcggc gggcgtgtcg accgaagcgg
      361 cgaccggagc tttcgcgtcg tgccgggccc gctcggcgga cgcgaggtgc ggatcgaggg
      421 cgaccactct tcggcctcgt acttcctcgc cgcggcggcg gtcgtcggtg gccgcgtgcg
      481 cgtctgcggc ctcacgccgg cgtcgcacca gccggacgcc gcgttgggcg ggatgctggc
      541 cgccgccggc tgtctggtgc gacgcggagc cgattggatc gaggtcgagc accacggccc
      601 gttgcgcccg ctcgacctcg acatggcaga ctcacccgac ctggtcccga ccgtcgcggt
      661 cgcggcgagc ttcgccggcg ggacttcggt cctgcgcaac atcgcccatc ttcggcacaa
      721 ggaatcggac cgtctgacga cgatcgcgac gaatctcgcg cgactcggtt gtccggcgga
      781 gatcggcgac gactcgctca cgatcgcgtc ccgcgggcga ccgacccgcg gagcgctgat
      841 cgagaccgcg tccgatcacc gaatcgcgat ggcgtttgcg ctggccgggc tcgccgtcga
      901 aggtgtcgcg gtcgacgacg cgcgctgcgt cgccaagtcg aacccgcgct tctggcagca
      961 gctggcgtgg ttgacggcgt gaccggcgat cagaactgga acgcgagccc ggcgcgccaa
     1021 ccggtcaata cgtaatcctg cgtgttgtag agcgccgacg tgccctggac gcgggcctcg
     1081 gtcgcgttct cggggatgcg gatgtcgttc ggcaacagca ccgcatcgag cagaccgacg
     1141 actccgtagg acagctgcag ctcgagcccg ctggtcagtc gcaggcgcgc cccggcttcc
     1201 gctgcggtgt gccaggtgct cttggtctgg ctcaaggaga gctcggcggg aacggtgaca
     1261 tcttccccgg ccgtgttgcc gaccaacgtc aggaactcgc cgctcgtcgt cgagacgtcc
     1321 tcgacgatga acgaaatctg gccgttgagg tagagctgca ggcgctgatt gaagaagttg
     1381 aaccgcgcct ccatcagtcc ggtcggtccg agcccgctcg cttcctggtg gaagttgagc
     1441 agccggagga aggctccgtc ggtgtagccg accccttcgc tggacgaccc gtggagccag
     1501 gcggccgccg acatgtttcc ctcgtagacg aagtagcgca ggccggcgaa ccaccgtccc
     1561 tgaaaaccac gcgggcccca gacgttgccg aagacgaagt cgtaggtctg cgcgcgattc
     1621 tgcagggagg tgaaggtctc ggccgtgaag tcgaacgcgg gagtcaggta gcgctgctcg
     1681 ccggcgtcga cgaagccgtt gccgttggcg tcgtcgcccg cgggcggtgg ggcgtcagtc
     1741 cagaccggcg gcgtgttgac cgaatgcagc tggccgtcat cgacggtgat cgtccaccac
     1801 aggagcgggt cgcggacgtc ttcggtcgcg acaccgaggt ccgggtcgag cgcggccaga
     1861 tagccctcgg cgtcgaagcg gtagccgtcc tcgctccagc cgttgaagat caggcggaag
     1921 tcgcctttgc cacccttcaa ccgccagccg atcgcggcgc ggccgtactg gtggtcgtcg
     1981 aacgagaccg tgttcacgct gagcgccgtg ctcaacgtct ggatcgagga atcgatgtcg
     2041 tcgagcgacc acgcgccgga gccggcttcg acgtagaggc cgaagtagtc ggtcgtcgct
     2101 tgctcggtcg gtgcggcctg gggctcggga gcgtcggtcg gcggtggcgg agtttcgtcc
     2161 tcttcggtct gcggcggggc ctcgggcggc ggagcgtcgt cctgggcggc gacggccgac
     2221 accgccagca gcaacagcag cgggctcacg ctcgcaagga accgcttaag cctcatcggt
     2281 gagccccctc ggcgccccgt cgcacgagaa cccgtgtttt gcagtgacac cgtgtcaaaa
     2341 gaccacccaa aatattcaaa gcggccggcc gcgtcaaggc gatcggcggt gtatcctggc
     2401 cggtggagcc gattctgctg catgtgtgca gagacgaaac gccggaccct gttgctgctc
     2461 gcctcctgct tgtgcgccgc tgcgacaccc gtcgcggggc agtcgaccgg ggaatcgctg
     2521 ccggaccgag agctcgagcg cttgctcgag ctgcaccaca ccgagcgcca gctggtccgt
     2581 ctggttcagg tgccggccgt ggtgacgacc cgggcgggca aggtcgtgcg cgggctcgac
     2641 gcgtccgact tcgtcgtcta cgaggaccac gtcccgcagg cgatccgtca cgtcacgacg
     2701 gagcacgaag agccggtctc gatcgccttc ctgctcgacg tttccggg
//
LOCUS       DASPGN010000170         9392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_496233, whole genome shotgun sequence.
ACCESSION   DASPGN010000170 DASPGN010000000
VERSION     DASPGN010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496233"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..1882
                     /locus_tag="VD788_04820"
     CDS             365..1882
                     /locus_tag="VD788_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HXV75621.1"
                     /translation="MRRRLVIGSLVALALVWVLPAGADDMRSDEEMDVTMVDEDVDES
                     EMKFRVTGQVRARYEYLWDYLEQSKDVNTNFDIAPYRAQIGVLGRFTDNVYGYIDLQA
                     FGAFGDSIPDSSSQNSGSQALDTDFLEATTVQLYQGFVDMKEIGGSNFDVRVGRAEHI
                     IGNELHMGNNDFYNGLTFDGARGWYDGGDRWDVGAYFYWAVERNTPVGNPLGVLAGPC
                     SPDVIVFPCTTSSLNPSNTDARVAGADFTWTFDQETGHYITGYAIQNTSRLEASFGGA
                     GDHELNTVGLLWKRDVAEERGFDWSAEAATQFGSITTTGNPDVDFGGYIVEGWLGYKL
                     AGAHRFRLGGLLASGVAEDDASDEFNGWVPLFTDYHQDNRLGNLDLFNGTLYTNGVVP
                     SSPGFGGIFGPVTFLTMSNVTNLNLRYEYERGKSYALIAVHDFQFTEELDPTGVNISD
                     IGQELDLLYRYQRTKHLTWEVAYSYFLPGDWYDEIYSPDDPEEISRFWIQAALQW"
     gene            complement(1955..2173)
                     /locus_tag="VD788_04825"
     CDS             complement(1955..2173)
                     /locus_tag="VD788_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75622.1"
                     /translation="MGQRSIPPETYLDLRIHLDRVSAAQKECFNALGALVSGDLSVDD
                     YRGLVDRQSKAQQAWVERHQKYFGVSGR"
     gene            2394..2873
                     /locus_tag="VD788_04830"
     CDS             2394..2873
                     /locus_tag="VD788_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXV75623.1"
                     /translation="MDPIDRTILRVLQDDGRRPRAEIAREVGLSTAAVHERIRKLERG
                     GVIRGYAALLDAERAGCDLLVFVEVFIEHPKYEPAFLAAIGTMPSVQECHRVTGAATC
                     LLKVRVADRRALQRLILDRINAMSGVRGTETVVVLSTTKETPQIDLGTAGEAVGGAP"
     gene            2870..4237
                     /gene="lat"
                     /locus_tag="VD788_04835"
     CDS             2870..4237
                     /gene="lat"
                     /locus_tag="VD788_04835"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR03251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HXV75624.1"
                     /translation="MNRRTVTPDGVIPTLERRILVDGFHVVLDLDRSSGSRLRDARDG
                     KEYLDCFSFFASSPLGINHPAMMEPAFLDRLARVAVNKVSNADFYTDVYAEFVEAVDR
                     IARPASMRYFFFIEGGALAVENALKAAFDWKVRLNRAAGVGGERGHGVLHLEHAFHGR
                     SGYTLSLTNTADPRKTMYYPKFDWPRIPSPAIRFPLDDAERARLHGAEDEALDQARRQ
                     LEQAQGGVAAIILEPIQGEGGDRHFRTRFLRGLRELADRYEALLIFDEIQTGVGMTGR
                     FWAFEHHDVEPDILVFAKKMQVGGLMAGDRIDRVPDNVFHVPSRINSTWGAHIVDMLR
                     ASRMLAVIRDEGLVERAEITGRRLLTGLAESAARHGGSIDNVRGRGLMCAIDLPGTEL
                     RDRAVRTAMTNGVIVLPCGERSIRFRPALTISEHEIDEAVARLDDTLAAVLVDARAEI
                     TPSGR"
     gene            complement(4243..5280)
                     /locus_tag="VD788_04840"
     CDS             complement(4243..5280)
                     /locus_tag="VD788_04840"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HXV75625.1"
                     /translation="MSGARRRLDWLSGGWVLLLAGSLVAGIVAWRVVPILLSSSRAVG
                     DGRNVATYGFALEPLDVPRETLAASGFAKDGIPALVDPAVMRGDAVRALNDSRRGKYL
                     VSDDRVIGVVLDGQTRAYPLRVLNWHEVVNDTLAGRPIAVTYHPLCDGAVVFDREVAG
                     EVVEFGVSGLLSDSNLLMFDRRADAEEESLWSQLLARAVAGPAAAAGHRLDVLPASLA
                     RWDVWLDTHPDTTVLEPEPRLFERYERNPYGNYFLTGRLRFPADPLPPDGSHPWMQPI
                     VVVERAGVRWQRLLGSSGGTATIEPLPGVRLRLDESTRPGSVLVEAPPGVAVFYSLWF
                     AWYATHPPGAQ"
     gene            complement(5283..6275)
                     /locus_tag="VD788_04845"
     CDS             complement(5283..6275)
                     /locus_tag="VD788_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HXV75626.1"
                     /translation="MRLALPFVLICCALIAAVAVLGALGRVADGAAFPELTLRDPDGE
                     RFEVGTLQGRVWVASFVAAVCLRECATTAEQLARLADGLPRDVPLVTFVLGGDSLWPE
                     PLAAAATRRGWIVAQGNDVDADSGVALEQAAIDYFGISRDRIEALVRRAPAAWIVAID
                     AQGRPLGAHALHDDADASIAATWGQVEFRTTLLANPMREARLFALTAVLVVAGIVLAR
                     LELVKIHPIFTVSASGITVILLGFGFRYVDSTIAAPARGSGWERPLYFAVLSTHTALC
                     AVIVVLALTLIFHAWRRQTERHRAIARWAAPAWVAVAATGSLVYFLLGRWFPAG"
     gene            complement(6272..7327)
                     /gene="hemH"
                     /locus_tag="VD788_04850"
     CDS             complement(6272..7327)
                     /gene="hemH"
                     /locus_tag="VD788_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HXV75627.1"
                     /translation="MPNDETGLLLINLGTPDSPGTPDVRRYLREFLLDPRVIDLPAWK
                     RQLIVRLFILPFRPKRSAHAYSLIWTPRGSPLLVQGRELTAKVQRRLGTAVRVELAMR
                     YGSPSIADALDAFRGGGVRKIVAFPLYPQYSSATTGSTVERLFEEAGRRWNTPYLQVI
                     PPFYDHPGFLDSWTEVARPVIARVEPERVFFSFHGLPERQILKGDERGDHCLRRADCC
                     ETIGEANRDCYRAQCHATARALADRLELDPRQRMVCFQSRLGREAWIRPYTDELLAEQ
                     AAAGCRRAVILSPAFVADCLETLEELGLRGVATWEQNGGETLELIPAPNASDSFADAV
                     VTIARESSRWLERDGAR"
     gene            complement(7402..8547)
                     /locus_tag="VD788_04855"
     CDS             complement(7402..8547)
                     /locus_tag="VD788_04855"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75628.1"
                     /translation="MSRRIGELLLDKGLITAVQLDQALRSQLFFGGHLGTCLIELGFV
                     SQDDLGHALAELLGLAYAGDAELDGVPDEVIRIFDTAMVEKYRAIPLRLEDKTLHLAV
                     IDPKSLGRLSALTGYRLIPWVTPESRLLEAMETHYGIQRSARYVRLSRGMVRQVPDEL
                     FAAGPGPGAADEAASGGDAGEPAVGSEADLEFGYGRPWREVAGELLDDDVRPSESVAP
                     AEPRAPVSIYQRMSRAEDKHELARLVLAYLAERMRGAILFTVRANTAAVWDSVGLEFD
                     RERSASLRFPVSRGSLFELMLGDGHYRGPLPDRPSCRWVHAALGLDPPEEMLLLPVYL
                     NERLVAVIYGDGGRSGEIRGSTELFVRLALKLGLGLSMLVLKIKIAS"
     gene            complement(8688..9113)
                     /gene="arfB"
                     /locus_tag="VD788_04860"
     CDS             complement(8688..9113)
                     /gene="arfB"
                     /locus_tag="VD788_04860"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HXV75629.1"
                     /translation="MVPIDASLAIPENELRITAERSSGPGGQHVNKVSTRIVLEFDLA
                     ASSALDPERKERIRRRLGRRITRDGRLRIVCQRHRSQAANRREAIERFAELIRRALAP
                     RRRRVATGVPPAERRRRLEQKRQRARLKDARRDGGEPDG"
BASE COUNT         1538 a         3246 c         3196 g         1412 t
ORIGIN      
        1 gaatcgcgcg ctcggcgagg caccgagacg gagcgttgtc ggcacgggtg caatcgtccc
       61 gttgcaggga tggtccaatc caatcacttg aatcgatccg gtcccttgaa cgaaaacgcg
      121 cggagcgtac cggcagggcg caaggagctg gacaaagcaa gtcgcttgtt ttcatccagt
      181 tgtccgtgac ggtcgcgggg tggtgacggc tggcaaccat cttgctgtga gaacgcagcg
      241 ctggagtgcc accacgggtg gcctgacgtt actggacgag ctcactgcca accgacgact
      301 cttgcatggg gcaacagttc gaggggggaa acggggactc cgtccggtag ttggaggaga
      361 ttcgatgcgg aggagattgg tcatcggcag tctcgtcgcg ctggccctgg tgtgggttct
      421 gccggccggc gcggacgaca tgcggtccga cgaagagatg gacgtgacga tggtcgacga
      481 ggacgtcgac gagagcgaga tgaagttccg cgtgaccggc caggtccgcg cccgctacga
      541 gtacctgtgg gactacctcg agcagagcaa ggacgtcaac accaacttcg acatcgcacc
      601 gtaccgggcg caaatcggcg tgttgggccg tttcaccgac aacgtctacg gctacatcga
      661 cctgcaggcg ttcggcgcct tcggcgacag catcccggac agcagtagcc agaactcggg
      721 ctcgcaggcg ctcgacaccg actttctcga ggcgaccacg gtccagctct accagggctt
      781 cgtcgacatg aaggagatcg gcggctcgaa cttcgacgtg cgagtcggcc gggccgagca
      841 catcatcggc aacgagctgc acatgggtaa caacgacttc tacaacggcc tgaccttcga
      901 cggcgcccgc ggctggtacg acggcggaga ccgctgggac gtcggggcct acttctactg
      961 ggccgtcgag cgcaacacgc cggtcggcaa tccgctcggc gtgctggccg gcccgtgctc
     1021 gcccgatgtc attgtgttcc cctgcaccac cagctcgttg aacccgtcga acaccgacgc
     1081 ccgcgtcgcc ggcgccgact tcacctggac cttcgaccaa gagaccggcc actacatcac
     1141 cggttacgcg atccaaaaca cctcgcggct ggaagcttcg ttcggcggcg ccggtgacca
     1201 cgagctcaac acggtcggcc tgctgtggaa gcgcgacgtg gccgaggagc gtggcttcga
     1261 ctggagcgcc gaggccgcga cccagttcgg cagcatcacg acgaccggga acccggacgt
     1321 cgatttcggc ggctacatcg tcgagggttg gctcggctac aagttggccg gtgcgcaccg
     1381 cttccgtctc ggtgggttgc tcgcctcggg cgtcgccgag gacgatgcga gcgacgaatt
     1441 caacgggtgg gtgccgctgt tcaccgacta ccaccaggac aaccgcctgg gcaacctaga
     1501 cctgttcaac ggcacgctct acacgaacgg cgtagtgccg tcgagccccg gcttcggcgg
     1561 catcttcggg cctgtcacct tcctcacgat gtcgaacgtg accaacctca acctgcgcta
     1621 cgagtacgag cggggcaagt cctacgcgct gatcgcggtg cacgacttcc agttcaccga
     1681 ggaactcgac ccgaccggcg tcaacatctc cgacatcggt caggagctcg acctgctcta
     1741 tcgctaccag cgcaccaagc acctgacctg ggaggtcgcc tactcctact tcctccccgg
     1801 cgactggtac gacgagatct actcgccgga cgacccggag gagatttcgc ggttctggat
     1861 ccaagccgcg ttgcagtggt aacgaccgaa ccggccgatc gaaggaaggg gcgggaaacc
     1921 gccccttctt ttttgaattc ggagcggcgc caggctagcg gccgctgacg ccgaagtact
     1981 tctggtgccg ttcgacccag gcctgctggg ccttgctttg gcgatcgacc agcccgcggt
     2041 agtcgtcgac gctcaggtcg cccgagacga gcgcgccgag tgcgttgaag cactccttct
     2101 gcgcggcgga gacccggtcg agatggatcc tcagatcgag ataggtttcg ggaggaatcg
     2161 atctctgccc catggaccct ccgtccggtc gccgaatgcg agtgtgtggc gctgacccgc
     2221 aagccattct agcgcccggg gatgcaagac ccaggttaac acgatgttaa attcgtgtta
     2281 cagcgaatcg gcttgtgtcc acaaaatcca taaattatgt gtagttgtct ccatttaggt
     2341 ggtatatttt cggcatgatg agtgttgtgc cgaatgatat tcagtctgcc aggttggacc
     2401 cgatcgaccg gacgatcctc cgggtgctgc aggacgacgg gcgtcggccg cgggcggaga
     2461 tcgcccgcga ggtcggcctt tcgacggcag ccgtccacga acggatccgc aagctcgaac
     2521 ggggcggcgt gatccgcggc tacgccgcgc tgctcgacgc cgagcgggcg ggttgcgatc
     2581 tgctggtgtt cgtcgaagtc ttcatcgagc acccgaagta cgagccggcc ttcctcgccg
     2641 ccatcggcac gatgccgtcc gtccaggagt gccatcgcgt caccggggcg gcgacctgcc
     2701 tgctcaaggt tcgcgtcgcc gaccggcgcg ccctgcaacg actgattctc gaccgcatca
     2761 acgccatgtc gggcgtccgc ggaaccgaaa cggtcgtcgt gctctcgacg accaaggaaa
     2821 cgcctcagat cgatctcggc accgccggcg aggcggttgg aggtgcacca tgaaccggcg
     2881 gaccgtcacg cccgacggcg tcatcccgac cctcgagcgt cgcatcctcg tcgacggatt
     2941 ccacgtggtg ctcgacctcg accgctcgtc cggcagccgc ctccgcgacg cgcgcgacgg
     3001 gaaggagtac ctcgactgct tttcgttctt cgcgtcgagc ccgctgggga tcaaccaccc
     3061 ggcgatgatg gagccggctt tcctcgaccg gctcgcccgc gtcgcggtga acaaggtcag
     3121 caacgccgac ttctacaccg acgtctacgc cgagttcgtc gaagcggtcg atcgcatcgc
     3181 gcggcccgcg tcgatgcggt acttcttttt catcgagggc ggcgcgctgg cggtcgagaa
     3241 cgcgctgaag gccgcgttcg actggaaggt gcggctgaat cgcgccgcgg gcgtgggcgg
     3301 cgagcgcggg cacggcgtcc tgcacctgga gcacgcgttc cacggtcgca gcggctacac
     3361 gctcagcctg accaacaccg ccgatccgcg caagacgatg tactacccga agttcgattg
     3421 gccgcgcatc ccgtcgccgg cgatccgctt tccgctcgac gatgccgagc gggcgcggct
     3481 tcacggggcg gaggacgagg cgctcgacca ggcccgccgg cagctcgagc aggcccaggg
     3541 cggcgtggcc gcgatcatct tggagccgat ccagggcgag gggggcgacc ggcacttccg
     3601 cacccgcttc cttcgcggcc tgcgcgagct ggccgaccgg tacgaggcgc tgctgatctt
     3661 cgacgagatc cagaccgggg tcggcatgac cggcaggttc tgggcgttcg agcatcacga
     3721 cgtcgaaccg gacatcctgg tcttcgccaa gaagatgcag gtcggcggac tgatggccgg
     3781 cgaccggatc gaccgtgtgc cggacaacgt gttccacgtc ccgtcgcgga tcaacagcac
     3841 ctggggcgct cacatcgtcg acatgctgcg cgcctcccgc atgctggcgg tgatacgcga
     3901 cgaggggctg gtcgaacgag ccgagatcac cggccgcaga ctgttgaccg gtctggccga
     3961 gtcggcggcg aggcacggcg ggtcgatcga caacgtgcgt ggccgtgggc tgatgtgcgc
     4021 aatcgacctg ccgggaacgg agctgcgcga ccgggcggtc agaaccgcga tgacgaacgg
     4081 ggtgatcgtg ctgccgtgcg gcgagcgatc gatccgcttc cgcccggcac tgacgatctc
     4141 ggaacacgag atcgacgagg ccgtcgcgcg gctcgacgac acgctggccg ccgtgctggt
     4201 cgacgcccgc gccgagatca cgccgagcgg acgctaggcg gactactgag cccccggcgg
     4261 atgtgtcgcg taccaggcga accacaggct gtagaacacc gccacgccgg gcggggcttc
     4321 gaccaggacc gaccccggtc gcgtcgattc gtcgagccgc aggcggacgc cgggaagcgg
     4381 ttcgatcgtc gcggtcccgc cgctcgaccc gagcaggcgc tgccagcgca cgcctgcgcg
     4441 ctcgacgacg acgatcggtt gcatccacgg atgcgagccg tcggggggga gcgggtcggc
     4501 ggggaagcgc agtctcccgg tgaggaagta gttgccgtac gggttgcgct cgtatcgttc
     4561 gaacagccgt ggctcgggtt cgagcaccgt cgtgtcggga tgcgtatcta gccagacgtc
     4621 ccagcgcgcg aggctggccg gaagcacgtc gagccggtga cccgccgccg ccgccggtcc
     4681 ggcgacggcg cgtgccagca gctggctcca caggctctcc tcttcggcgt ccgcccggcg
     4741 gtcgaacatc aacagattgg aatcggacag cagcccgctg acgccgaatt cgacgacctc
     4801 gcccgccacc tcgcgatcga agacgaccgc accgtcgcac agcgggtgat aggtcaccgc
     4861 gatcggccgc ccggcgagcg tatcgttgac cacctcgtgc cagttgagca cacgtaacgg
     4921 gtaggctcgc gtctgcccgt cgagcacgac gccgatcacc cgatcgtcgc tgaccaggta
     4981 cttgccgcgg cggctgtcgt tgagcgcgcg caccgcgtcg ccgcgcatca cggcggggtc
     5041 gacgagcgcc ggaatcccgt ccttggcgaa tccggaggcg gccagcgtct cgcgcgggac
     5101 gtcgagcggc tcgagcgcga agccgtaggt cgcgacgttc cggccgtcgc cgacggcacg
     5161 cgaggagctg agcaggatcg gcacgacgcg ccaggcgacg atgccggcca ccagcgatcc
     5221 ggcgagcaac aggacccagc cgccgctgag ccagtcgagc cggcgccgag cgcccgacat
     5281 cctcagcccg ccgggaacca gcgaccgagc aggaagtaga cgaggctccc cgtcgccgcc
     5341 acggcgaccc aggccggcgc cgcccaccgc gcgatcgcgc ggtggcgttc ggtctgtcgg
     5401 cgccacgcgt ggaagatcag ggtcagcgcc agcacgacga tcaccgcaca cagcgcggtg
     5461 tgggtgctca acaccgcgaa gtacagcggt cgttcccaac cggagccgcg cgccggggcg
     5521 gcgatcgtgg aatcgacgta gcggaagccg aagccgagca ggatgacggt gatcccgctc
     5581 gcggacacgg tgaagatcgg gtggatcttg accagctcga gccgggcgag cacgatgccg
     5641 gccacgacca gcacggcggt cagggcgaac agccgcgcct cgcgcatcgg attggcgagc
     5701 aacgtcgttc gaaactcgac ctggccccag gtcgctgcga tcgacgcgtc ggcgtcgtcg
     5761 tgcagcgcgt gggcaccgag cggccggccc tgcgcgtcga tcgcgacgat ccacgccgcc
     5821 ggcgcccggc ggaccagggc ctcgattcgg tcacgggaga tgccgaagta gtcgatcgcc
     5881 gcctgctcga gcgcgacgcc gctgtcggcg tcgacgtcgt tgccctgcgc gacgatccac
     5941 ccccggcggg tcgcggcggc agcgagcggc tcgggccaca ggctgtcgcc gccgaggacg
     6001 aaggtaacga gcggcacgtc gcgcggcagg ccgtccgcca gtcgagccag ctgctcggcc
     6061 gtcgtcgcgc actcgcgcag gcagacggcg gcgacgaaac tcgccaccca gacccgaccc
     6121 tgcagcgtgc cgacctcgaa tcgctcgccg tccggatcgc gcagcgtcag ctcgggaaag
     6181 gcagcgccat cggcgacgcg cccgagcgcc ccgaggaccg cgacggccgc gatcaacgcg
     6241 cagcagatca gcacgaacgg cagcgccagc ctcatcgcgc gccgtcccgc tcgagccagc
     6301 ggctcgactc gcgagcgatc gtcacgaccg cgtcggcaaa gctgtcgctc gcgttgggcg
     6361 ccgggatcag ctcgagcgtc tcgccgccgt tctgctccca cgtcgccacg ccacgcaatc
     6421 ccaactcttc gagcgtctcg agacagtccg cgacgaacgc cgggctgagg atcaccgcac
     6481 ggcggcagcc ggcggctgcc tgctcggcga gcaactcgtc ggtgtaaggc cggatccacg
     6541 cctcgcggcc gagcctggac tggaagcaga ccatgcgttg ccgcgggtcg agctccagcc
     6601 ggtcggccag cgcccgcgcg gtcgcgtggc actgcgcccg gtagcaatca cgattcgcct
     6661 cgccgatcgt ctcgcagcag tcggcgcgcc ggagacaatg gtcgccccgc tcgtcgccct
     6721 tgaggatctg ccgctccggc agaccgtgga agctgaaaaa cacccgttcg ggctcgacgc
     6781 gcgcgatcac cggtcgcgcc acctcggtcc acgagtcgag gaagcccggg tggtcgtaga
     6841 atggcgggat cacctgcagg tagggcgtgt tccagcgccg gcccgcttcc tcgaacagtc
     6901 gctcgaccgt cgagccggtc gtcgccgagc tgtactgggg gtacagcggg aaggcgacga
     6961 tcttccgcac gccgcccccg cggaacgcgt cgagcgcgtc ggcgatcgac ggcgagccgt
     7021 accgcatcgc gagctcgacg cggaccgccg tgccgaggcg ccgctgcact ttcgcggtca
     7081 gctcgcgccc ctggaccagc agcggcgacc ctcgcggcgt ccagatcagg gaataggcat
     7141 gtgccgaccg cttgggtcga aacggcagga tgaacagccg gacgatcagc tggcgtttcc
     7201 aggcggggag gtcgatcacc cgcgggtcga gcaggaactc gcgaaggtaa cgccgaacgt
     7261 cgggcgtccc gggcgagtcc ggcgtcccga ggttgatcag cagcaggccg gtctcgtcgt
     7321 ttggaatcgc ggacctccgg gaagatcatc cgccacgcac gacgcggacc tccattctat
     7381 cagcccgccg gagcgcggcc gtcaggaggc gatcttgatc ttgagcacga gcatgctcaa
     7441 gcccagaccg agcttgagcg ccagcctgac gaacagctcg gtcgagccgc ggatctcgcc
     7501 cgatcgaccg ccgtcgccat agatcacggc gaccaaccgc tcgttgagat agaccggcag
     7561 caacagcatt tcttccggcg gatcgagacc cagcgccgcg tggacccagc ggcagctcgg
     7621 tcggtccggc agcggcccgc gataatgccc gtcgcccagc atcagctcga acagcgatcc
     7681 cctcgagacg gggaagcgca acgacgccga gcgttcgcgg tcgaactcga gcccgaccga
     7741 atcccagacc gcagcggtat tggccctgac cgtgaacagg atcgcgccgc gcattcgctc
     7801 ggcgaggtag gcgagcacca gccgtgcgag ctcgtgcttg tcctcggcgc ggctcattcg
     7861 ctggtagatc gacaccggcg ctcgcggctc ggccggggcg accgactcgc tcggccgcac
     7921 gtcgtcgtcg agcagctcgc cggcgacctc gcgccacgga cgtccgtagc cgaactcgag
     7981 atcggcctcg gagccgacgg cgggctcgcc cgcgtcgccg ccggaggctg cctcatcggc
     8041 ggcccccggt ccgggcccgg cggcgaacag ctcgtccggc acctggcgga ccatgccccg
     8101 gctcagccgg acgtaccgcg cgctgcgctg gatgccgtag tgcgtctcca tcgcctcgag
     8161 caggcggctt tcgggcgtga cccacgggat caacctgtac cccgtgagtg ccgacaaccg
     8221 cccgagcgac ttggggtcga tcacggccag atgcagcgtc ttgtcctcca ggcgcagcgg
     8281 gatcgcccgg tacttctcga ccatcgcggt gtcgaagata cggatcacct cgtcggggac
     8341 cccgtcgagc tccgcgtcgc ccgcgtaggc caagccgagc agctcggcca gcgcatgccc
     8401 cagatcgtcc tgtgacacga aaccgagctc gatcagacag gtgccgaggt ggccgccgaa
     8461 gaacagctga gagcgcaacg cctggtcgag ctggaccgcc gtgatcagcc ccttgtcgag
     8521 cagtaattcg ccgatccgtc gtgacatcgt ctgctgggtc cgccgatttg ccccccggat
     8581 tcggcgaata tagatcgcgg gccggcgccg agccatccgc ccggagcgac tgcgcggggc
     8641 acacgatgcc gccggggctc accgcgggct cgcagacccc gcgcggctca cccgtccggt
     8701 tcgccgccgt cgcgccgcgc gtccttcaat ctcgcgcgct ggcgcttctg ctccaatcgc
     8761 cggcgccgct ccgccggcgg cacgccggtc gccacgcgcc ggcgacgcgg cgcgagcgcc
     8821 cggcggatca gctcggcgaa ccgctcgatc gcttcgcggc ggttcgccgc ttggctgcga
     8881 tggcgctggc agacgatacg cagccggccg tcgcgcgtga tgcgccggcc cagccgccgt
     8941 cggatccgct ccttccgctc cgggtcgagc gccgacgagg cggccaggtc gaactcgagc
     9001 acgatccgcg tgctgacctt gttgacgtgc tggcctcccg ggccgctgct gcgctcggcg
     9061 gtgatgcgca gctcgttctc ggggatggcc agactggcgt cgatggggac cacgtgcctc
     9121 ggccacgggg ggatcgatcc cgcgcgggcg agtataggac cggccgcgcc cgcttgcctt
     9181 tcccggtgcg tttcctaagt ttcttggttg taggcggatt ccgctccgcc gccacgggtg
     9241 gccatgcaga tcgagtcgat gcccagtgtg ccgcgccggg gcgagatcgc gtcggccagc
     9301 ggcgaacggc gaaaggtcgt gctgctgcgc ggcgcgttgc tcgtcgccgt cggcgcattg
     9361 ttgatccagt ccggcgccgc ggggccgctg cc
//
LOCUS       DASPGN010000171        15404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_262823, whole genome shotgun sequence.
ACCESSION   DASPGN010000171 DASPGN010000000
VERSION     DASPGN010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 15404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15404
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262823"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VD788_04865"
     CDS             <1..1100
                     /locus_tag="VD788_04865"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HXV75630.1"
                     /translation="TKKNLPVIQQDKPIEWEAAQGTRVEIELEAVYQKGRRSVDDYVE
                     QTALANPHVAIVYSPPRDEPKPYARVTSELPKESVEIKPHPYGVELGVLLRMVKETQA
                     KNLRGMLTAEFSRVSPAVATEICRLAGLKPEAVPARLSGPQVESIFRAIADVRILAPP
                     TACLSPIGEQLLGQGLEQRIGADFSTAVTRPPAVYRGNPFQVEVGLSYGGALSAEEPV
                     ELYRYANRVPLQYQQSACAISKAAVSIDWKKYGLQQARGALPVAPMILFVHIASVWVP
                     FTSESKEAVASYPEILKEIRLGLMEAGRRLGVFVRRRRRESDEHKKRTYIEKYIPHIG
                     DALQEILGLSDERRDKTVENLKLILERSRKV"
     gene            complement(1109..3040)
                     /locus_tag="VD788_04870"
     CDS             complement(1109..3040)
                     /locus_tag="VD788_04870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HXV75631.1"
                     /translation="MIQLDNVTRRFGARVLFEGLSWTVKPRARIGLVGPNGAGKTTLL
                     RLLAGDDEPDLGAVHRAGSLEVGFLPQEVETVVGDSVLAVALAGFAEVSRLDARLAEL
                     ARRLARARDDDEESRRLAAEYGTLRGRLEALDGDRVEARATAILSGLGFAAGSLSQPP
                     AVMSGGWRMRAVLARLLVGTPDLLLLDEPTNHLDLEQIDWLERYLAEYEGAFVAVSHD
                     RYFLNRIVDTIVELDRGELTPFRGDYDAYVETKQRREAALDQATRHQAREIAKVERFI
                     ERFRYKNTKARQVQSRVRTLSKLERVRVEPKTSSIRFRFPPAPRSGDVVARVEAVDKR
                     FGELEVYRRASLLLRRGDRVALVGDNGAGKSTLLKLLAGRLAPDAGRVELGHQVELHY
                     YAQHQLDALDPARSVLEELEAVASPEDRPRLRGLAGRFLFHGDDVAKRVAVLSGGEKA
                     RLALGKMLLRPANLLLLDEPTNHLDLVSREVLEDALDEFGGTVVLISHDRYFINRVVT
                     RIVHVVVGSLQPYDGDYDEFVRQRDRCALVAPEPDAAGPEADGPRRERRAERRREAEE
                     RNRRYRDRKRIEEALRPIEREVAGLERLQRELEAEQARPEVYADPAEARRVAREKERV
                     VARLEQLYQRWEEVARGLV"
     gene            3153..4226
                     /locus_tag="VD788_04875"
     CDS             3153..4226
                     /locus_tag="VD788_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75632.1"
                     /translation="MIAGFAGRTSLFQPAGSIGMLVVLLAAGGSTIRAETLRYAIDLP
                     AGRAVTYQLTFAVRHPGSVEVHAEWSGDRALSLKLTPPGNKYGTLLRSGPSPLDVTTD
                     ADGELGEWRLRVHALARRDAGEGLLVIRTPAAIVEVPRPVTADATPDGPLESWMEPPD
                     LPDRVPSSWLTFLDAIERYRTIIAGEGDAPVTDVCQWQSALMRYLAERRGGLLADGVT
                     PARPTADLLTRIAATVDLVERLRTSDDPLVAGPPPTDAALRNAWVKLRTTRIDPVEDE
                     LDEVLRLVQRAYAAELDEEPWPVRFVSCLTACERYFEQSARLGETRAENRDLALAQWP
                     RILAASRALAELAAVEARPAPPR"
     gene            complement(4241..5902)
                     /locus_tag="VD788_04880"
     CDS             complement(4241..5902)
                     /locus_tag="VD788_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HXV75633.1"
                     /translation="MPIVVVCLSVYAAGYLLYARYLGRRLFDLRPDALTPARALDDGI
                     DYVPTHRFVLFGHHYASITGLSPMLGPAIAVIWGWLPAMTWVVVGAIAVGCVHDFGAL
                     VVSARAEGKSIGFVAEGLVGPRAKPLLHAIIFFGVSLAMGVFAYVIARLFTPEFYPQA
                     VLPSVVILVLAVSIGWLVRRRGLRLWPLVVLAFVLMLLTIGLSPAVGWAWNSLGGWIV
                     VLLVYAWSASVLPVWSLLQPRDFLNSLVLYLGLGLTFIGLFVLDPRFAAPVIDPRPPG
                     APALFPFVFIVIACGAASGFHALVASGTTAKQLARETDARAIGYGGMIGESLLGLAAV
                     LACTAGFSSRTAWSEHYASWQALASLGSQMSSFIDGAAGFVAALGVPLEQARSLIAVM
                     VVSFALTTLDSATRLLRYNIEEIGESARVRALSNRYLSSTVAVAAIAFFAFYEIDGQS
                     AGLALWQLFGSTNQLLAGLTLLVVSLYLIRRRRPALPYVLPMLFMMASTLTAMVMKLG
                     DYLREQQWLLLGVGSMITAIAVWLIVESGFALRRFRLEGSARVVR"
     gene            complement(5979..8429)
                     /gene="lon"
                     /locus_tag="VD788_04885"
     CDS             complement(5979..8429)
                     /gene="lon"
                     /locus_tag="VD788_04885"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXV75634.1"
                     /translation="MPAEIGVLPLRNSVLFPHAIMPISVGRRKTLNLLDDVVGDNKLI
                     AVVSQRNPAEDDPEPSGMYRVGTIARILKVVKLSGNNVNLIIQGLKRVRVDRFTASEP
                     FMKAQVTAMDDVVDDGVELEALVRSLVNQFQKLVQVSQNISSDLGEMVLSAGAGEPAR
                     LSDLAASVLTISAEEKIGLLERNNVKDRVQKLVETITRELELQEISSKIQSQVADEVG
                     KTQRQYYLREQMKAIQKELGEEDERQAEIDELRKKIEDAKMPEEPRKAAEKELDRLSK
                     MPPQAAEYTVARTYLDWLVGLPWSVETEDKLDVPAAKTVLDEDHFDLEKVKDRILEFL
                     AVRSLKHDLKGPILCLAGPPGVGKTSLGRSIARAMGRKFIRISLGGVRDEAEIRGHRR
                     TYIGSLPGRIVQGMKRAGTNNPVFMLDEVDKLGADFRGDPSSALLEVLDPEQNFSFSD
                     HYLEVPFDLSRVFFICTANVLDTIPAALRDRMEVLRIPGYTEEEKVEIARAHLVPRQR
                     EEHGLKAEQIEFSEDAIRTVINDYTREAGVRNLDREIANLTRKVARKYVEGDTAQVVL
                     DGDRVHELLGPVRYFRELSERTDRSGVAIGLAWTQVGGEILFVESSRMKGKGKITITG
                     RLGDVMKESALAAMTWIRANAARLGIDERFFETSDFHVHIPAGAIPKDGPSAGVTLTC
                     SLVSLLTGIPVQADLAMTGEVTLRGKVLPVGGIKEKVIAAKSAGIKRVLLPRRNEKDL
                     EDVSEAVRQAMQFEFVDDTEGLLELAFGEALRQRLNDLPPVPERIARRSDEHETPIAV
                     VVSRDAEVEDDPVDVPRA"
     gene            complement(8615..10324)
                     /locus_tag="VD788_04890"
     CDS             complement(8615..10324)
                     /locus_tag="VD788_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75635.1"
                     /translation="MSNQIWSFDELVRLTRSANAEVRFWAVDRLIRHFPGECCDPIAD
                     LLLDEHEATPAAVARHLGEHGGPKHHAILVRGFRVLRGLTPGYSLHALVRLGYPHAVE
                     LASDALKRGDLNQPVLGAIVEALAELGTAPARALIRESVERRPELLAEPAAFRGALRI
                     AEAEELGEVIAGFVRALRWHGTHRAGESFRTLMDTLRIDDAGWCFRTGPSGHIELRKT
                     IKAVESGYDCDIGAAMGEVTIRQVGRRFRAGNVGDVVRALAEWTRGATADFPTDGRND
                     QPRRIAAAVGAFADPQLSGEAAGYGQQFQQWLLGFQLSAAFAVARKLNPELLLRRARG
                     RLDALLELAELETAHQLGELPMAIAAVCRDDETNARKAQEWCLRMLEAQGPFFPKVIA
                     LDVLGDLRAVHFIPEVMDYLSDENSYVYGAAERALSKMGEALIAPALERIETGTIDPD
                     AAHSVLVLFCDLGTHASYEAVLSYFDWFMDAVGPGTTAEWSSLFGMEELIEPLRDWLD
                     EDTPLVGQALLMLGAIHNVPIPEEDEILQAIEDERARQAAMTGSDAETADGPNGEGGN
                     YLM"
     gene            complement(10411..13419)
                     /locus_tag="VD788_04895"
     CDS             complement(10411..13419)
                     /locus_tag="VD788_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV75636.1"
                     /translation="MRLVAWSIAACCGAWSMAQGQATTGTLTGTVTGTDERPVADAVV
                     QARSDEHGALRTAVTDRGGNYTIASLRPGNWSLVARAADGRRSPVSAVTVRLQETLRV
                     DFLVAASLEERVEVAAERPLIDPRETAGRLRVSGDQADTLPTAARNVTDLALLDSAVQ
                     AAPPGTFVGERASVFTVNGQSGRSNSFLIDGLDNNDQISGTTQNSFYSQLVIDEFVLL
                     KNQYAPEFGRASGGVMNVLTRSGSNELQWDGFVQGSSYRWNGPGEFVSELPREPDSQD
                     SSSGLLGGFRLGGPIARDRTFYFLAYEHQQIDEAVPFVGIDREGRPGGVFLAPNRNDN
                     LFLRTDFNLSASNALMVRLSYNDRTTSGVNVGGVQTPEYGFTIDERDSDLAATLTTIL
                     SPRLVNEVRLLAASSSFDQRANSSRPGVARPSGLFGGNNLNYQLRDETRLALVENVSY
                     RAGDHTLKVGVDVIASRTDLDARFNPNGNFIYNSDLPFEPGDCGNLFLDDVFEAERLG
                     TLPLVDCPGQIDVDDDGDGEFNESGNLDSYPVVFTLLFGQPTAILDNTNFALFVQDHW
                     QAGKRWVLDYGLRYDVSTYELPESARVDSTVPNGGATRDLNNVAPRVGFAFRPYDDGR
                     MVLRGGAGMFYDKLVLGFPAVAAVTSGAKIGLSFPQGFAFEITEQFVEDHGIAATIPE
                     LLLDNPFLDELIMQFSTAAELETPYTVQANLGLDLRLGRSSALSFNAMHNRGYHTPLM
                     VDLNPVSGLRPVGVDCRPENLDPELEVGLPCHLKDPTTGSIAAITTDGRSWYAAAGVD
                     FRVIGARSWSSVSYTLSKAEDLGFDPLKGGIALPPDSDDIDGERARADADRRHRFVWS
                     GDTDLPWAGLRMSGVLQLSSGLPFNVTTGTDDNLDGILSDRPAGVGRNSGEDTPLAPV
                     NALRAEHGLPPVESLDEPSFAQLDLRIYRPFSWRTERGRGEVYLQVINLFDRENGGLV
                     EGRVASLNFGRVITLAGPPRTLEFGFRIGY"
     gene            complement(13452..>15404)
                     /locus_tag="VD788_04900"
     CDS             complement(13452..>15404)
                     /locus_tag="VD788_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXV75637.1"
                     /translation="LLEPTIRRVRWTEESRDGIASLRADRRRVGMGWVRLPGGRREVV
                     LERALVSREVSGEWIAERLEHRWIDPRFGIVALIGGPVDTDGRRRPGAEHVVYTEEVG
                     RGPAGLRVYDFMMDLPVFSRLSYGYDRKGACTVGGNPCIDDGDCTAGSGDICTIPVSL
                     LTPDAHATIGELIAAGSWDFSANDFDQQRYELAATISPVDAAETCSYDKCGFTRPGVK
                     LGRQDKNFVEPAEVDITTTVTEREDRTDGGGQQTDVTLWLRAGVNKEGQTGSLGQGES
                     RICYVDDGRTEVPLWRFPHQDPSGWFWQLGDSWSSDSNLATPELDPFLCENDIFVHTC
                     PNSCGLFCPNYIAACDGYSGTQGSAVIGEGPVTLPSGHTFHSLLVRNVAEFCVFIGSS
                     CASPVFRVRTVVYLWEVPWLGTVARLTSAQNVADYTSWTTVEETDLKFGLFPPRSLTI
                     GAVTDESIELSWDPGLDTHRIDAFRVYWSTIAGSYDPATSVIQPAASGHSAVIGGLEP
                     ATEYFFTVTAISEFTNPATSQTTTYESLMLPMTAPGVPQPVPIEVSATTTGAGCAPTT
                     EVGGVVVDRGPGADIEICWSSVVDDCLDGYQILGADSPESPDDFTPVVEDTGLVNCAI
                     VPPSRSYFLVVAKGAGGNGPWGHYGR"
BASE COUNT         2567 a         5334 c         5071 g         2432 t
ORIGIN      
        1 acacgaagaa gaacctgccg gtgatccagc aggacaagcc gatcgaatgg gaagcggcgc
       61 agggcacgcg ggtcgagatc gagctggagg cggtgtacca gaaggggcgg cgctcggtgg
      121 acgattacgt cgagcagacg gcgttggcca atccgcacgt cgcgatcgtc tactcgccgc
      181 cgcgagacga gccgaagccg tacgcgcgcg tcacctccga gctccccaag gagtcggtcg
      241 agatcaagcc ccacccgtac ggcgtcgagc tcggcgtatt gctgcgcatg gtcaaggaga
      301 cgcaagcgaa gaatctacgc gggatgctga ctgcggagtt cagccgggtg agcccggcgg
      361 tcgcgaccga gatctgtcgg ttggccggcc tcaagcccga agccgttccg gcgcgactgt
      421 cgggcccgca ggtcgagtcg atcttccgcg cgatcgcgga cgtgcggatc ctcgcgccgc
      481 cgacggcgtg cttgtcgccg atcggcgagc aactgctggg acaggggctc gagcagcgga
      541 tcggcgcaga cttttcgacc gccgttaccc gtccgcctgc cgtctatcgc ggcaatccgt
      601 tccaagtcga ggtcggcctg tcgtacggcg gcgcgctctc agccgaggag ccggtcgagc
      661 tctatcgcta cgccaaccgg gtgccgctgc agtaccagca atcggcgtgt gcgatctcga
      721 aggccgcggt ctcgatcgat tggaaaaaat acgggctgca gcaggcccgc ggcgcgctgc
      781 ctgtcgcgcc gatgatcctg ttcgtccaca tcgcctcggt ctgggtgccg ttcacctcgg
      841 agtcgaagga agcggtcgcg tcctaccccg agatcctgaa ggagatccgc ctcggactga
      901 tggaggccgg ccgccggctc ggcgttttcg tccgtcggcg ccgccgggag tccgacgagc
      961 acaagaagcg cacctacatc gagaagtaca tacctcacat cggcgacgcg cttcaggaga
     1021 ttctcgggct gagcgacgag cggcgcgaca agacggtgga gaacctgaag ctgatcctcg
     1081 agcgcagccg caaggtctga gcgcgccgtc acaccaggcc tcgggcgacc tcctcccagc
     1141 gctggtagag ctgttccagc cgagcgacga cgcgctcctt ctcgcgagcg acccgcctcg
     1201 cctcggccgg atccgcatag acctccggcc gtgcctgctc cgcctcgagc tcgcgctgga
     1261 gccgctcgag gccggccacc tcccgttcga tcggccgcag cgcttcctcg atgcgcttgc
     1321 gatcgcggta acgtcgattg cgttcctcgg cttcgcgccg tcgctccgcg cgtcgctcgc
     1381 gccgtggccc gtccgcctcg gggcccgcgg cgtcgggctc cggggcgacc aacgcgcagc
     1441 ggtcgcgctg ccgcacgaac tcgtcatagt cgccgtcgta cggctgcagc gagccgacga
     1501 cgacgtggac gatgcgggtc accacgcggt tgatgaaata gcgatcgtgg gagatcagca
     1561 cgacggtccc gccgaactcg tcgagggcgt cttccagcac ctcgcggctg acgagatcga
     1621 ggtggttcgt cggttcgtcg agcaacagca gattggccgg tctcaacagc atcttgccca
     1681 gcgccaggcg ggccttttca ccacccgaga gcaccgcgac ccgtttggca acgtcgtcgc
     1741 cgtggaacag gaacctgccg gcgagcccgc gcagccgcgg tcggtcctcg ggcgatgcga
     1801 ccgcctcgag ctcctcgagc accgagcgcg cgggatcgag ggcgtccagt tggtgttgcg
     1861 catagtagtg cagctcgacc tggtgcccca gctcgacccg gcctgcgtcg ggcgcgagcc
     1921 gcccggccag cagtttcaac aacgtcgact tgccggcacc gttgtcgccg accagcgcca
     1981 ctcggtcgcc ccgccgcagc agcaggcttg cacggcggta gacctcgagc tcgccgaacc
     2041 gcttgtccac ggcttcgacc ctggccacca catcgcccga acgcggtgcg ggagggaaac
     2101 gaaaccgaat ggacgacgtc ttaggctcga cgcgcacccg ttcgagcttg ctcagagtgc
     2161 gcacgcgcga ttgcacctgc cgagccttgg tgttcttata gcggaagcgt tcgatgaacc
     2221 gttcgacctt ggcgatctcg cgcgcctggt ggcgcgtcgc ttggtcgagc gccgcctctc
     2281 gccgttgctt ggtctcgacg taggcgtcgt agtcgccgcg gaacggggtc aactcgccac
     2341 ggtcgagctc gacgatcgtg tcgacgatcc ggttgaggaa gtagcggtcg tgcgagacgg
     2401 cgacgaacgc cccctcatac tccgcgaggt accgttcgag ccagtcgatc tgctcgaggt
     2461 cgaggtggtt cgtcggctcg tcgagcaaca gcagatccgg tgtgccgacc agcagccggg
     2521 ccagaacggc acgcatccgc cagcctccgc tcatcaccgc cggcggctgg ctcaggctgc
     2581 cggccgcgaa accgaggccc gacaggatcg ccgtggcccg cgcctcgacg cggtccccgt
     2641 cgagcgcctc gagccgcccg cgcagcgtgc cgtactccgc ggccagtcgc cggctctcct
     2701 cgtcgtcgtc ccgggcacgg gccaaccgcc gcgcgagctc ggcgaggcgt gcgtcgagtc
     2761 gcgacacctc ggcgaatccg gcgagcgcaa cggcgagcac cgagtcgccc acgaccgtct
     2821 cgacctcctg cggcaggaag ccgacctcga gcgaaccagc acgatggacc gcgccgaggt
     2881 cgggctcgtc gtcgccggcg agaaggcgca gcaacgtggt cttgccggcg ccattcggtc
     2941 cgaccagacc gattcgcgcc cgtggcttga cggtccacga caggccctcg aacaacaccc
     3001 gcgcgccgaa tcgtcgggtc acgttgtcca gttgaatcac gaaagtgtcg tccgagggcc
     3061 gaccacccgg tcgcgccgac ggcgaccttt gtccgacgct cgaccgggca ttatattcgg
     3121 cccgaggccg cgacccgggc gtcgacgaca cggtgatcgc tggtttcgcg gggaggacgt
     3181 cgttgttcca gccggccgga tcgatcggga tgctcgtcgt gttgctggcc gccggcggaa
     3241 gcacgatccg cgcggaaacg ttgcgctacg cgatcgacct cccggccggc cgcgcggtga
     3301 cttaccagct gacgttcgcc gtccggcacc ccggttcggt cgaggtgcac gccgaatggt
     3361 cgggggatcg cgcgctgtcg ttgaagttga cgccgccagg gaacaagtac ggcacgctgc
     3421 tgcggtccgg tccctcgccg ctcgatgtca ccaccgacgc cgatggcgag ctcggcgagt
     3481 ggcgtctacg ggttcacgcg ctcgcccgac gggatgccgg cgaagggctg ctcgtcatcc
     3541 ggactccggc ggcgatcgtc gaggtgccgc gaccggtgac cgccgacgcc acgcccgatg
     3601 gaccgctcga gagctggatg gaaccgcccg acctacccga tcgggtgccg tcgagctggc
     3661 tgacctttct cgacgcgatc gagcgctacc gcacgatcat cgccggcgag ggagatgcgc
     3721 cggtgaccga cgtctgtcag tggcagtccg ctctgatgcg ctacctggcg gaacgtcgcg
     3781 ggggcctgct ggccgacgga gtcacaccgg cacggccgac ggccgatttg ctcacgcgga
     3841 tcgccgccac cgtcgacctc gtcgagcgtc tgaggacgag tgacgatccg ctggtcgccg
     3901 gtccgccgcc gacggacgcc gcgctgcgaa acgcctgggt caagctgcgc acgacgagga
     3961 tcgacccggt cgaagacgag ctcgacgagg tcctccgcct ggtccagcgc gcctatgccg
     4021 ccgagctcga cgaggagccg tggccggtcc gtttcgtcag ttgcctgacc gcctgtgagc
     4081 gctacttcga gcagagcgcc cgcctcggcg agacgcgcgc cgagaatcgc gacctggccc
     4141 tggcccagtg gccacggatc ctcgccgctt cgcgcgcgct tgccgaactc gccgcggtcg
     4201 aggcgcgacc ggccccgcct cgctgagcag cacgaaccgt tcagcggacg acccgcgccg
     4261 agccttcgag acgaaaccgt ctgagcgcga agccgctttc gacgatcagc cacaccgcga
     4321 tcgcggtgat catcgacccg acaccgagca gcagccactg ctgttcgcgt aagtaatcgc
     4381 caagcttcat caccatcgcg gtcaacgtac tcgccatcat gaacaacatc ggtaggacgt
     4441 agggcagcgc tggacgacgc cgtcggatca ggtacagcga gacgacgagc agcgtgaggc
     4501 ccgcgagcag ttggttggtt gatccgaaca actgccacag cgccagtccc gccgactgac
     4561 catcgatctc gtagaaggcg aagaacgcga tggcggccac cgcgacggtc gacgacaggt
     4621 atcggttcga cagcgccctg acccgggccg actcgccgat ctcttcgatg ttgtagcgca
     4681 acagtcgcgt cgccgagtcg agggtcgtca acgcgaagct cacgaccatc acggcgatca
     4741 acgagcgggc ctgctcgagc gggacgccga gcgcggcgac gaagccagcg gcgccgtcga
     4801 tgaacgagct catctggctt ccgaggctgg ccaacgcttg ccacgacgcg tagtgttcgg
     4861 accacgccgt gcgcgacgag aagccggccg tacaggcgag caccgccgcc aagccgagca
     4921 gtgactctcc gatcatcccg ccgtagccga tcgcgcgagc gtccgtctcg cgcgccagtt
     4981 gtttggcggt cgtgcccgag gccaccaggg catgaaaccc cgatgccgcg ccgcaggcga
     5041 tcacgatgaa cacgaagggg aacagcgccg gtgcgcccgg tggccgggga tcgatcaccg
     5101 gcgcggcaaa gcgcgggtcg agcacgaaca ggccgatgaa cgtcaacccc aagccgaggt
     5161 agagcaccag cgagttgaga aagtcacgcg gttggagcag cgaccacacc ggcagcaccg
     5221 aggccgacca ggcgtagacc agcagcacga cgatccaccc gccgagcgag ttccacgccc
     5281 aaccgacggc cggcgacagc ccgatcgtga ggagcatcag cacgaaggcg agaacgacga
     5341 gcggccacag ccggagaccg cgacgcctta ccaaccagcc gatcgacacg gccagcacca
     5401 ggatcaccac ggacggcagc accgcctgcg ggtagaactc gggcgtgaac aggcgtgcga
     5461 tcacgtaggc gaacacgccc atcgccagcg acacgccgaa gaaaatgatc gcatggagta
     5521 gaggcttggc gcgcggtccg accaggccct cggcgacgaa cccgatcgac ttgccctcgg
     5581 cgcgggccga gacgaccagc gcgccgaagt catggacgca tccgacggcg atcgccccga
     5641 cgacgaccca cgtcatcgcc gggagccagc cccagatcac cgcgatcgca ggccccaaca
     5701 tcggcgaaag accggtgatc gaggcgtaat gatgaccgaa cagcacgaag cgatgggtcg
     5761 gcacgtagtc gatcccgtcg tcgagcgccc gggccggagt cagcgcgtcg ggccgcagat
     5821 cgaacaacct ccggccgaga taccgtgcgt acagcaggta gccggcggcg tacaccgaca
     5881 agcagaccac gacgatcggc acgacgcgca acggccactc cgccccggga cgggtcgccc
     5941 attctacgtc tgaatcggtc gcgagccgcc gttgcgcctc aggcgcgggg tacgtcgacc
     6001 ggatcgtcct cgacttccgc gtcgcgcgag acgacgaccg cgatcggcgt ctcgtgttcg
     6061 tccgagcgtc gcgcgatccg ctccggtacc ggcggcaagt cgttcagccg ttggcgaagc
     6121 gcttcgccga aggcgagttc gagcagcccc tcggtgtcgt cgacgaactc gaactgcatc
     6181 gcctggcgca ccgcctccga cacgtcctcg aggtccttct cgttgcgccg gggcaacagc
     6241 acccgcttga tccccgcgga cttggccgcg atcactttct ccttgatgcc gccgaccggg
     6301 agcaccttgc cgcgcaaggt gacctcgccg gtcatcgcca gatcggcctg caccggaatc
     6361 ccggtcaaca gcgagaccag cgagcaggtc agcgtgaccc cggccgacgg gccgtccttc
     6421 gggatcgcgc cagccgggat gtgcacgtga aagtcgctcg tctcgaagaa ccgctcgtcg
     6481 atgcccaggc gcgcggcgtt ggcacggatc cacgtcatcg ccgccagcgc cgattccttc
     6541 atcacgtcgc cgagccgtcc ggtgatcgtg atcttgccct tgcccttcat ccgcgacgac
     6601 tcgacgaaca ggatctcccc gccgacctgc gtccacgcga gaccgatcgc gactccgctg
     6661 cggtcggtcc gttccgacag ctcgcggaag taacggaccg ggcccagcag ctcgtggact
     6721 ctgtcgccat cgagcacgac ttgtgccgtg tcaccctcga cgtacttgcg ggccaccttg
     6781 cgtgtcaagt tcgcgatctc gcgatcgagg ttgcgcacgc ccgcctcgcg cgtgtagtcg
     6841 ttgatcaccg tgcggatcgc gtcctcgctg aactcgatct gctcggcctt caacccgtgc
     6901 tcctcgcgct gtcgcgggac gaggtgcgct cgcgcgatct cgaccttctc ctcctcggtg
     6961 tagccgggaa tgcgcagcac ctccatccgg tcgcgcaacg cggcgggaat cgtatcgagc
     7021 acgttggcgg tgcagatgaa gaagacccgg ctcaggtcga acggcacctc gagatagtgg
     7081 tccgagaacg agaagttctg ctcggggtcg agcacctcga gcagcgccga cgacggatcc
     7141 ccgcggaagt cggcacccag cttgtcgacc tcgtcgagca tgaacaccgg gttgttggta
     7201 ccggcgcgtt tcatgccctg gacgatgcgc cccgggagcg agccgatgta ggtccggcgg
     7261 tgcccgcgga tctcggcctc gtcgcgtacg ccgccgagcg agatgcgaat gaacttgcgc
     7321 cccatcgcgc gcgcgatcga gcgtccgagc gacgtcttgc cgacccccgg cggcccggcg
     7381 agacacagga tcggaccctt gaggtcgtgc ttcagcgacc gtacggcgag gaactcgagg
     7441 atgcggtcct tcaccttctc cagatcgaag tggtcctcgt cgagcacggt cttggccgcg
     7501 gggacgtcca gtttgtcttc ggtctcgacc gaccacggca ggccgaccaa ccaatcgagg
     7561 taggttctcg cgacggtgta ctccgccgcc tgcggcggca tcttggacag ccggtccaac
     7621 tccttctcgg ccgccttgcg cggctcctcg ggcatcttgg cgtcttcgat cttcttgcgc
     7681 agctcgtcga tctcggcctg gcgctcgtcc tcctcgccca gctccttctg gatcgccttc
     7741 atctgctcgc gcaggtagta ttgccgctgc gtctttccga cctcgtcggc cacttgagac
     7801 tggatcttgc tcgagatctc ctgcagctcg agctcccgcg tgatcgtctc gacgagcttc
     7861 tgcacgcggt ccttgacgtt gttgcgctcg agcagcccga tcttctcctc ggcggagatg
     7921 gtcagcaccg aagcggcgag gtcgctcagt cgcgccggct ccccggcccc cgccgacaac
     7981 accatctcgc cgaggtcgct cgatatgttc tgcgagacct gcacgagctt ctggaattgg
     8041 ttgaccaggc tgcggaccag cgcttcgagc tcgactccgt cgtcaacgac gtcgtccatc
     8101 gcggtgacct gggccttcat gaacggctcg gacgcggtaa agcggtcgac gcgaacccgc
     8161 ttcagaccct ggatgatcag attgacgttg tttccactca gtttgacgac cttgaggatc
     8221 ctggcgatcg tgccgacacg gtacatcccg ctcggctccg gatcgtcctc cgccgggttc
     8281 cgctgcgaca ccacagcaat cagcttgttg tcgccgacga cgtcgtcgag caggttcagc
     8341 gtcttgcgac gaccgacgga gatcggcatg atcgcgtgcg gaaacaacac gctgttgcgc
     8401 aacggcagga cgccgatttc ggctggcacg tggagagctt ccatgctcgt tgaccgtcct
     8461 ttcgaggcga ttcggccccg ctctctcagt atagtgtcct cgggcggtga tggcccgact
     8521 cgattcgtca ctcgagcagc gcggggccgc cgccggtgat gggcccataa gacgtggtgc
     8581 accgactgtc aagcccgcgc gcaccgcggt cgggctacat caggtagttg ccgccctcgc
     8641 cgttcgggcc gtcggcggtc tcggcgtccg agcccgtcat tgccgcctga cgcgcacgct
     8701 cgtcctcgat cgcctgcagg atctcgtctt cctcggggat cgggacgttg tggatcgcgc
     8761 ccaacatcag cagcgcctgc ccgaccaacg gcgtatcctc gtcgagccag tcgcgcagcg
     8821 gctcgatcaa ctcttccatg ccgaacaagc tgctccattc ggcggtggtg cccggaccga
     8881 cagcgtccat gaaccagtcg aagtagctca gcaccgcctc gtaggaggcg tgggtgccga
     8941 ggtcgcagaa caacacgagg acgctgtgcg ccgcgtccgg atcgatcgtc ccggtttcga
     9001 tccgctcgag cgcgggtgcg atcagcgcct cgcccatctt cgacagggcc cgctcggcgg
     9061 cgccatagac gtacgaattc tcgtcggaca ggtagtccat cacttccggg atgaaatgga
     9121 ccgcccgcag gtccccgagc acgtcgagcg cgatcacctt gggaaagaac gggccctgcg
     9181 cctcgagcat ccgcaggcac cattcctgcg ccttgcgtgc gttcgtctcg tcgtcccggc
     9241 agacggcggc gatcgccatc ggcagctcgc cgagctggtg cgccgtctcg agctccgcca
     9301 gttcgagcag cgcgtcgagt ctgccgcggg cacgccggag caagagctcg gggttcagtt
     9361 tccgggcgac ggcgaacgcg gccgaaagct ggaagccgag cagccattgt tgaaactgct
     9421 gaccgtatcc ggcagcctct ccactgagct gcgggtcggc gaacgcaccg accgcggcgg
     9481 cgatccgtct gggctggtcg tttcggccgt cggtcgggaa gtccgctgtc gcgccgcggg
     9541 tccactcggc gagggcgcgg acgacgtcgc cgacattacc ggcgcggaac cgccgtccga
     9601 cctgccggat cgtcacttcg cccatcgccg cgccgatgtc gcagtcgtag ccggattcca
     9661 ccgccttgat cgtcttgcgc agctcgatgt gaccggacgg acccgtgcga aaacaccagc
     9721 cggcgtcgtc gatacgcagc gtgtccatca acgtgcggaa cgattccccc gcccggtgcg
     9781 taccgtgcca gcggagcgcg cggacgaagc cggcgatcac ctcacccagc tcctcggcct
     9841 cggcgatccg caacgcgccg cgaaacgcgg ccggctcggc caacagctcg ggtcggcgct
     9901 cgacgctctc gcggatcagc gcgcgcgccg gggcggtgcc cagctcggcc agcgcctcga
     9961 cgatcgcgcc gagcaccggt tggttgagat cgcctcgctt cagtgcgtcc gacgccagct
    10021 cgaccgcgtg cgggtagccg aggcggacca gcgcgtgcag gctgtaaccg ggcgtcagcc
    10081 cgcggagcac gcggaagccg cgcaccagga tcgcgtgatg cttggggccg ccgtgctcgc
    10141 cgaggtgtcg cgcgaccgcc gcgggcgtgg cctcgtgttc gtcgagcaac agatccgcga
    10201 tcgggtcgca gcactcaccg gggaagtgcc ggatcagccg atcgaccgcc cagaagcgca
    10261 cctcggcgtt cgccgaacgg gtcaagcgga ccagctcgtc gaagctccag atctggttgg
    10321 acatcccgcg cggttccccg tcagccgaag cgaagcgtgg cattctagcc ctgatcccgg
    10381 cggcgcgccc gctggccgac cgccatctct tcagtatccg attctgaacc cgaactcgag
    10441 cgtgcgcggt ggaccggcca gcgtgatcac cctgccgaag ttgagcgagg cgacgcgacc
    10501 ctcgaccaga ccgccgttct cgcggtcgaa caggttgatc acctgcaggt agacctcgcc
    10561 gcgaccacgc tcggtgcgcc agctgaatgg ccgatagatg cgaagatcga gttgggcgaa
    10621 cgagggctcg tcgagcgatt cgaccggcgg cagaccgtgc tcggcgcgca gggcgttcac
    10681 cggggcgagc ggggtgtcct ccccgctgtt ccggccgacg cccgcgggcc ggtcgctcaa
    10741 gatgccgtcg agattgtcgt cggtgccggt cgtcacgttg aacggcaggc cactcgacaa
    10801 ctgcagcacg cccgacatgc gcagcccggc ccatggtaga tcggtgtcac cggaccagac
    10861 gaaacgatgg cggcggtcgg cgtcggcccg cgcgcgctcg ccgtcgatgt cgtccgagtc
    10921 ggggggcaac gcgatcccgc ccttgagcgg atcgaacccc agatcctctg ccttggacag
    10981 cgtgtacgag accgagctcc acgacctggc gccgatgaca cggaagtcga cgccggcggc
    11041 ggcgtaccac gagcgcccgt cggtcgtgat cgcggcgatc gaaccggtcg tcgggtcctt
    11101 gaggtgacac ggcagaccga cttcgagctc gggatcgaga ttctccgggc ggcaatcgac
    11161 gccgaccgga cgcaggccgg agaccgggtt gaggtcgacc atcagcggcg tgtggtagcc
    11221 gcggttgtgc atcgcgttga acgacagcgc cgagctgcga cccagacgca gatccaaacc
    11281 gaggttggcc tggacggtgt acggcgtttc gagctcggct gcggtcgaga actgcatgat
    11341 caactcgtcg agaaacgggt tgtcgagcag cagctccggg atcgtcgccg cgatgccatg
    11401 gtcctcgacg aactgctcgg tgatctcgaa ggcgaaaccc tgcggaaacg acagcccgat
    11461 cttcgcgccg gaggtgaccg cagcgacggc ggggaaaccc agcaccagct tgtcgtagaa
    11521 cattcccgca ccgccgcgca gcaccattcg gccgtcatcg tacggccgga acgcgaagcc
    11581 gacccgcggg gcgacgttgt tgaggtcgcg cgtggcaccg ccgttcggca ccgtcgaatc
    11641 gacgcgggcc gattcgggca gctcgtacgt gctcacgtcg tagcgcaggc cgtagtccag
    11701 cacccagcgt ttgccggcct gccagtgatc ctggacgaac aacgcgaagt tggtgttgtc
    11761 gaggatcgcg gtcggttgtc cgaacagcag cgtgaacacc accgggtagc tgtcgaggtt
    11821 gcccgactcg ttgaactcgc cgtcgccgtc gtcgtcgacg tcgatctgcc ccgggcagtc
    11881 gacgagcggc aacgtaccga gccgttcggc ctcgaacacg tcgtcgagaa aaaggttgcc
    11941 acagtcaccg ggctcgaatg gcaggtctga attgtagatg aagttcccgt tgggattgaa
    12001 ccgcgcgtcg agatcggtgc gcgatgcgat cacgtcgaca ccgactttca gcgtgtgatc
    12061 gccggcccgg tagctgacgt tctcgaccaa cgccaggcgg gtctcgtcgc gcaactgata
    12121 gttgaggttg ttaccgccga atagaccgga cggccgtgcc acccccgggc gcgacgagtt
    12181 ggcccgttgg tcgaacgacg agctggcggc cagcagccgg acttcgttga ccagacgcgg
    12241 cgagaggatc gtggtcaatg tggccgccag atcggaatca cgctcatcga tggtgaagcc
    12301 gtactccggt gtctgcacgc cgccgacgtt gaccccgctc gtcgtccggt cgttgtagga
    12361 caagcgcacc atcagcgcgt tgctcgccga caggttgaag tccgttcgca agaagaggtt
    12421 gtcgttccgg ttcggcgcga ggaagacccc accgggccgg ccttcgcggt cgatgccgac
    12481 gaacgggacc gcttcgtcga tctgctggtg ctcgtaggcc aggaagtaga aggtccggtc
    12541 ccgtgcgatc ggtcctccga gacggaatcc gccgagcaga ccgctcgatg agtcctgcga
    12601 gtcgggctcc cgcggcaact cggagacgaa ctcgcccggt ccgttccacc gatagctcga
    12661 accctggacg aacccatccc attgaagctc gttcgagccg ctacgcgtca acacgttcat
    12721 cacgccaccc gaggcacgac cgaactccgg cgcgtactgg ttcttcaaca acacgaactc
    12781 gtcgatcacg agttgcgagt agaagctgtt ctgagtcgtg ccgctgatct ggtcgttgtt
    12841 gtccaggccg tcgatcagaa agctgttgga gcgaccggac tgaccgttga ccgtgaatac
    12901 cgacgcgcgc tcaccgacga aggtgccggg tggtgcggcc tggaccgcgg agtcgagcag
    12961 cgccaggtcg gtgacgttgc gcgccgcggt cggcagcgtg tcggcctggt ctcccgagac
    13021 acgcagccgg ccggcggtct cgcgcggatc gatcagcgga cgttcggccg ccacctcgac
    13081 ccgttcctcg agcgaggcgg caacgaggaa atcgacccgc agtgtctcct gcaggcggac
    13141 cgtgaccgcg ctcaccggac tccgtctgcc gtccgcggcc cgcgcgacga gcgaccagtt
    13201 cccgggccgt aacgacgcga tcgtgtagtt tccgccgcga tcggtcaccg cggtccgcag
    13261 cgcgccgtgc tcgtcggagc gtgcctgaac caccgcgtcc gccaccggcc gttcgtcggt
    13321 gccggtcacc gtgccggtga gcgttccggt cgtcgcctgg ccctgcgcca tcgaccacgc
    13381 gccacagcag gcggcgatcg accacgcgac caacctcacg cggtgcgccg acacgcttct
    13441 cgtcgcggtc gtcaacgacc gtaatgcccc catggcccgt tgccacccgc tcccttcgcg
    13501 acgacgagga agtaggaacg cgacggcggg acgatcgcgc agttgacgag cccggtgtcc
    13561 tccaccacgg gagtgaagtc gtcaggcgac tcgggcgaat cggcacccag gatctgatag
    13621 ccgtcgagac agtcgtcgac gaccgagctc cagcagatct cgatgtcagc gcccggcccg
    13681 cggtcgacga ccacaccgcc gacctcggtg gtgggcgcgc agcccgcgcc cgtcgtcgtg
    13741 gccgagacct cgatcgggac cggctgcggg acaccgggag ccgtcatcgg caacatcagg
    13801 ctctcgtagg tcgtcgtctg cgacgtcgcc ggattggtga actccgagat cgcggtgacg
    13861 gtgaagaaat actcggtcgc cggctccagc ccgccgatca ccgcgctgtg acccgaagcg
    13921 gcgggctgga tcacgctggt cgccgggtcg tagctccccg cgatcgtgct ccagtagacg
    13981 cgaaaggcgt cgatccgatg ggtgtcgaga ccggggtccc aggagagctc gatcgattcg
    14041 tcggtcaccg cgccgatcgt gagcgagcgt ggtgggaaca ggccgaactt caggtcggtc
    14101 tcttcgaccg tggtccacga ggtgtagtcg gccacgtttt gcgccgaggt cagcctggcg
    14161 acggttccca accacggtac ctcccacagg tagaccaccg tgcggacgcg gaacaccgga
    14221 ctcgcgcagg acgacccgat gaacacgcag aactccgcca cgtttcgcac gagcaacgaa
    14281 tggaaggtat ggcctgaggg tagcgtcacc ggcccctcgc cgatgaccgc gctgccctgg
    14341 gttcccgaat acccgtcgca ggcggcgatg tagttcgggc agaacagccc gcagctgttg
    14401 gggcaggtat ggacgaagat gtcgttctcg cacaggaacg gatcgagctc gggcgtggcg
    14461 aggttgctgt cgctcgacca cgagtcgccg agctgccaga accagccgga cggatcctga
    14521 tgaggaaaac gccacaacgg tacttcagtc cgcccgtcgt cgacgtagca gatccggctc
    14581 tcaccctggc ccaggctgcc ggtctgccct tctttgttga ccccggcccg cagccacagc
    14641 gtgacgtcgg tctgctgccc gccgccgtcc gtccggtcct cgcgctcggt cacggtcgtc
    14701 gtgatgtcga cctccgccgg ttcgacgaag ttcttgtcct gacggccgag cttgaccccg
    14761 ggacgggtga agccgcactt gtcgtagctg cacgtctccg ccgcgtcgac cggcgagatc
    14821 gtcgcggcca gctcgtatcg ctgctggtcg aagtcgttgg ccgagaagtc ccacgatccc
    14881 gcggcgatca gctcgccgat cgtcgcgtgg gcgtccggtg tgagcagcga gaccggaatc
    14941 gtgcagatgt ccccgctacc cgccgtacag tcgccgtcat cgatgcacgg gtttcctccg
    15001 acggtacacg cacccttccg gtcgtagccg tacgagagac gggagaagac cggaagatcc
    15061 atcatgaagt cgtagacccg gagaccggcc ggcccacgcc cgacctcctc ggtgtacacc
    15121 acgtgctcgg ctcccggccg gcgacggccg tcggtgtcga ccggaccacc gatgagcgcg
    15181 acgataccga atcgcggatc gatccagcga tgctcgagcc gctcggcgat ccactcgccg
    15241 gatacctcgc gcgatacgag cgcccgctcg agcacgacct cgcgccggcc gccgggcagc
    15301 cggacccacc ccatccccac gcggcgccga tcggcgcgaa gcgacgcgat cccgtcgcgg
    15361 gactcctccg tccagcggac gcggcgaatc gtcggctcga gcag
//
LOCUS       DASPGN010000172         5218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_144816, whole genome shotgun sequence.
ACCESSION   DASPGN010000172 DASPGN010000000
VERSION     DASPGN010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144816"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /gene="truA"
                     /locus_tag="VD788_04905"
     CDS             complement(<1..763)
                     /gene="truA"
                     /locus_tag="VD788_04905"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXV75638.1"
                     /translation="MPSMFAYRLDLEYEGTRYSGWQAQANARTVSGELRRALVAAGAQ
                     VVELGGAGRTDAGVHAAGQTAHLRLAARIDPGAIRRAVNELLAPDIHLLALRPASPRF
                     HARHQAVLRSYVYQVSRRRTAMAKRFVWWIKRPIRVEAIRDAARELVGRRDFSRLCEA
                     PSRQASTVVVVDRVEVAEDGDLILFRFVASHFLWKMVRRTVGALVRVGTGELTGAQWR
                     RLVDPRSDPAAEGSPAAAWTAPPSGLFLERVAYPGE"
     gene            845..1399
                     /gene="efp"
                     /locus_tag="VD788_04910"
     CDS             845..1399
                     /gene="efp"
                     /locus_tag="VD788_04910"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXV75639.1"
                     /translation="MKANHLRRGMVIKFENDLWSVFEATHQTPGNLRARMQTKLKNLR
                     TGTMKDVRFRSEDEVEKAHLELARKQYLYRDPDGFHFMDVDNYEQFVISDETLGDAVH
                     YMQADMVLQVLMHEGQPVGVELPATVDLQVVETTPEVKGATASAQRKPAKLETGLVVQ
                     VPAFVAEGERIRVRTEDGQYVERA"
     gene            1483..2040
                     /locus_tag="VD788_04915"
     CDS             1483..2040
                     /locus_tag="VD788_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HXV75640.1"
                     /translation="MGSRVEVPPGLGERARRELTDLIDGLPDAGARRRRIAKSAIRAP
                     DVLNEAQAYDKPAFFSRGMRVELPGCTMLFLSGTASVDERGATIHDGDFDAQCLRTFR
                     NLSRLLAAERADWRDVVRTTCYLRDIERDYDRFNELRSWFLGTVGLDPLPASTGIQAR
                     LCRTDLLVEIEMIAITPREDGAASG"
     gene            2037..2876
                     /gene="aroF"
                     /locus_tag="VD788_04920"
     CDS             2037..2876
                     /gene="aroF"
                     /locus_tag="VD788_04920"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXV75641.1"
                     /translation="MSPTLPDLGILRRCWSGFVEAGSTRTVEVGQVVIGGGRPVVIGG
                     PCAVESLEQTLEIAEVVRDAGGQILRGGAYKPRTNPHSFLGLGRHGLEILAEARRVTG
                     LPFVTEVLDPRRVEEVASFADMLQIGSRSMHNYPLLTEVGRAGRPVLLKRGWSATLEE
                     WLCAAEYVAKEGNDRIVLCERGIRSAVHWSYAQSVLDLNVLQPAREATPLPLIVDPSH
                     ATGHWKLVAAMSRAALAAGAHGLLVEVVAHGVDRAALSSDAMQGVPPRVFAEIVAAAE
                     ATR"
     gene            2873..5152
                     /locus_tag="VD788_04925"
     CDS             2873..5152
                     /locus_tag="VD788_04925"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75642.1"
                     /translation="MRRAALAVVVGALVTTAVGAASITVDYPSPNSIFPPEMEAPTFL
                     WHDPTPDVDHWEVSIGFDGESKPVLRVEAAGDPPAPGEIDERCVAVTNEIHRPTDYQA
                     SARAWKPAPEIWATIRSAAGGRSVHVGFSGRRADGGEAGSRGHVELTISPDPVGAPIF
                     YRDVPLMPSAGADGVIKPVDQSALPLIGWRLRDLSRPESRLLLESMPTCANCHSFSSD
                     GRTLAMDIDGPQGDKGAYAVVDIEPETVIRDEDVISWNRFDDKPRGFNTLGFMARVSP
                     DGSRVVATVNEALYVRNFWDYRFSQVFFPTRGILAWYDRTTGGIRALPGADDPDYVHC
                     NAVWTPDGEELIFARSPARDPYEPGRPPATYAGDPNETQIRYDLYRMPFDDGRGGAPT
                     PIAGASANGSSNSFPKVSPDGRWLVWTRSDNGLLMRPDSRLWIVALDGGQPRELESNL
                     TTMNSWHSFSPNGRWLVFSSKANTPYTQMFLTHLDDNGHASPAILVENSTAANRAVNL
                     PEFVHTDYESFHSIAVPAVDHYAHFHRGNELARAGRYDEAVAAYREALEGEQQDWRIN
                     DWRIHDSLSKILTQIGQRELALHHVRRSLELNPSNLEMRTNLGYLLAERGEPRQALEQ
                     FDLALRLAPAEAQNWYNRATVRMQLGDDAGAISDYTEAIERNPRHARALIARGILLEA
                     AGRTGEALADFDRTVGLDPNNPTALFLRGRIRAARGERLAALADFETALAVAPPDWTD
                     RPRVERALERLRSGPPGGE"
BASE COUNT          826 a         1840 c         1806 g          746 t
ORIGIN      
        1 gctcgccggg gtaggccacc cgttcgagga acaagccgga gggtggtgcg gtccacgccg
       61 cggccggtga cccctcggcc gccgggtccg atcgcggatc gaccaaccgc cgccactggg
      121 cgccggtcaa ctcgcccgtg cccacccgca ccagggcgcc gacggtgcgc cgtaccatct
      181 tccacagaaa gtgcgaggcg acgaagcgga acaggatcag gtctccgtcc tcggcgacct
      241 cgacccggtc gaccacgacg accgtgctcg cctggcggga cggtgcctcg cacagacggc
      301 tgaagtcgcg gcggccgacc agctcgcggg cggcgtcgcg gatcgcctcg acgcggatcg
      361 gacgcttgat ccaccagacg aatcgcttgg ccatcgccgt ccgccggcga gagacctggt
      421 acacgtagct gcgcaagacg gcctggtgcc gggcgtggaa ccgcggcgat gccggccgga
      481 gcgcgagcag gtggatgtcg ggtgccagca actcgttgac cgcgcggcgg atcgcgccgg
      541 ggtcgatccg ggccgcgaga cgcaggtggg cggtctgccc cgcggcgtgc acgccggcgt
      601 cggtccgtcc ggccccaccc agctcgacga cttgcgctcc ggccgccacg agcgcgcgtc
      661 gcagctcgcc cgagacggtg cgggcgttgg cctgggcctg ccagcccgaa tagcgcgtgc
      721 cctcgtactc gaggtccaac cggtaggcga acatcgacgg cattatgccc gtcgcccgcc
      781 gagccctcca ggcgacgggc gctgtccgct acggtgtgcc aagatggcgc cggaggttcg
      841 tcgcatgaaa gccaaccacc tgcgccgcgg catggtgatc aagttcgaga acgatctatg
      901 gagcgtcttc gaggccacgc atcagacgcc gggcaacctg cgagcgcgga tgcaaaccaa
      961 gctgaaaaac ctccgcaccg gtacgatgaa ggacgtgcgc tttcgctccg aggacgaggt
     1021 cgaaaaagcc catctcgagt tggcgcgaaa gcagtacctc taccgtgacc ccgacggctt
     1081 ccacttcatg gacgtcgaca actacgagca gttcgtcatc tccgacgaga cgctcggtga
     1141 cgccgtccac tacatgcaag ccgacatggt gctgcaggtg ctgatgcacg aaggtcagcc
     1201 ggtcggcgtc gagctgccgg cgaccgtcga tctccaggtg gtcgagacca cgcccgaggt
     1261 caagggcgcg accgcctcgg cgcaacgcaa accggccaag ctcgaaacgg gtctcgtcgt
     1321 gcaggtgccg gcattcgtcg ccgaaggtga gcggatccgc gtgcgtaccg aggacggaca
     1381 gtacgtcgag cgcgcgtagc ggcaaatgcg accctgacgg tcctctttgg ctcggtcctt
     1441 ccgcgtatct tctgtgacgt gccgttcggg cggaggggat cgatgggcag ccgcgtcgaa
     1501 gtgccgcccg ggctcggcga gcgagcccgg cgggaactca ccgatctgat cgacgggctg
     1561 ccggatgccg gagcaaggcg gcgacgcatc gccaagagcg cgatccgcgc acccgacgtg
     1621 ctcaacgaag cgcaggccta cgacaagccg gccttcttca gccgcggcat gcgggtcgag
     1681 ttgcccggtt gcacgatgct gtttctctcc gggacggcca gcgtcgacga gcgcggcgcg
     1741 acgatccacg acggggattt cgacgcgcag tgcctgcgta cctttcgcaa cctcagccgg
     1801 ctgctcgcgg ccgagcgggc cgactggcgc gacgtcgtgc gtacgacctg ctacctgcgc
     1861 gacatcgagc gcgactacga tcggttcaac gagctccgca gctggttcct cggcacggtc
     1921 gggctcgatc cgctgccggc cagcaccgga atccaggccc ggttgtgccg gacggacctc
     1981 ctcgtcgaga tcgagatgat cgcgatcacg ccgcgcgagg acggagccgc gagtggatga
     2041 gcccgacgct gcctgatctc ggcatcctca gacgttgctg gagcgggttc gtcgaggcgg
     2101 gaagcacccg caccgtcgag gtcgggcagg tcgtcatcgg cggggggcgc cccgtcgtca
     2161 tcggtgggcc gtgcgcggtc gagtccctcg agcagacact cgagatcgcc gaggtggtcc
     2221 gcgacgccgg cgggcagatc ctccgcggcg gggcgtacaa gccgcgcacc aacccccact
     2281 cctttctagg gctcggtcga catgggctcg agatcctcgc cgaggcccgc cgggtgactg
     2341 gcctgccgtt cgtcaccgag gtgctcgatc cgcgacgcgt cgaggaggtc gcgtcgttcg
     2401 ccgacatgct gcagatcggg tcgcgatcga tgcacaacta cccgctgctg accgaggtcg
     2461 gccgcgcggg aagacccgtg ctgctcaagc gcggctggag cgccacgctc gaggagtggc
     2521 tgtgcgcggc ggagtacgtg gccaaggagg gcaacgatcg gatcgtgctc tgcgagcgcg
     2581 gcatccgctc ggcggtccac tggagctacg cgcagagcgt gctcgacctc aacgtgttgc
     2641 agccggcgcg cgaggcgacg ccgctgccgc tgatcgtcga cccgtcgcat gcgaccggtc
     2701 actggaagct ggtggcggcg atgagccgtg cggcgctcgc cgccggggcg cacgggctgc
     2761 tggtcgaggt cgtggcgcac ggggtcgatc gagcggcgct ctcctcggac gcgatgcagg
     2821 gcgtaccccc acgcgtattc gccgagatcg tcgccgccgc cgaggccacg cgatgaggcg
     2881 cgcggcgctc gcggtcgtcg tcggcgcgct cgtcacgacc gccgtcgggg cggcctcgat
     2941 caccgtcgac tatccgtccc cgaattcgat cttcccaccg gagatggagg ccccgacctt
     3001 cctctggcac gacccgacgc cggacgtcga tcactgggag gtgtcgatcg gtttcgacgg
     3061 cgagtcgaag ccggtgctgc gcgtcgaggc agccggcgac ccgccggcgc cgggagagat
     3121 cgacgagcgc tgcgttgccg tcaccaacga gatccaccgg ccgaccgact accaggccag
     3181 cgcgcgagcc tggaagccag cgcccgagat ctgggcgacg atccgcagcg cggccggcgg
     3241 gcggagcgtc cacgtcgggt tctccggccg gcgggcggac ggcggcgagg ccggctcgcg
     3301 cggtcacgtc gagctgacga tctcgcccga cccggtcggc gcgccgattt tctaccgtga
     3361 cgtaccgctg atgccgtcgg ccggcgccga cggtgtgatc aagccggtcg accagtcggc
     3421 gctgccgctg atcggctggc ggctccgcga cctgtcgcgg cccgaaagcc gcctgctgct
     3481 cgagagcatg ccgacctgcg ccaactgcca tagcttctcg agcgacggcc gcacgctggc
     3541 gatggacatc gacgggccgc agggcgacaa gggcgcctac gcggtcgtcg acatcgagcc
     3601 cgagacggtg atccgcgacg aggacgtcat cagttggaac cgcttcgacg acaagccgcg
     3661 cggcttcaat acgctcggct tcatggcgcg cgtgagcccg gacggcagcc gcgtcgtggc
     3721 gacggtcaac gaggcgctct acgtgcgcaa cttctgggac taccgcttct cccaggtgtt
     3781 ctttccgacg cgcggcatcc tcgcctggta cgaccggacg accggcggga tccgcgcgct
     3841 gcccggcgcg gacgatccgg actacgtcca ctgcaatgcg gtgtggacgc cggacggtga
     3901 ggagctgatc ttcgccaggt cacccgcgcg cgacccgtac gagccgggcc ggccgccggc
     3961 aacctacgcg ggtgacccga acgagacgca gatccgctac gacctctatc gcatgccgtt
     4021 cgacgacggg cgcggcggcg cgccgacgcc gatcgcgggc gcctccgcca acggctcgag
     4081 caactcgttc cccaaggtct cgccggacgg acgctggctg gtctggaccc ggagcgacaa
     4141 cgggctgctg atgcgaccgg acagccggct gtggatcgtc gcgctcgacg gcggccagcc
     4201 gcgcgagctc gagtcgaacc tgacgacgat gaactcgtgg cactcgttct cgcccaacgg
     4261 gcgctggctc gtcttttcgt ccaaagccaa cacgccctac acccagatgt tcctcaccca
     4321 cctcgacgac aacggacacg cgagcccggc gatcctcgtc gagaacagca ccgcggcgaa
     4381 ccgcgcggtc aacctgccgg agttcgtcca caccgactac gagagctttc actcgatcgc
     4441 ggtccctgcc gtcgaccact acgcccactt ccaccgtggc aacgagctgg cgcgcgccgg
     4501 tcgttacgac gaggccgtcg ccgcttatcg cgaggcgctc gagggcgagc aacaggactg
     4561 gcggatcaac gactggcgca tccacgacag cctgagcaag atcctcacgc agatcggcca
     4621 gcgcgagctg gccctgcatc acgtccgccg gtcgctcgag ttgaatccgt ccaacctcga
     4681 gatgcggacg aatctcggct acctgctcgc cgaacgcggc gagccgcggc aggcgctcga
     4741 gcagttcgac ctggcgctgc gactggcgcc ggccgaagcg cagaactggt acaaccgggc
     4801 gaccgtgcga atgcagctcg gcgacgacgc cggggcgatc tcggactaca ccgaggcgat
     4861 cgagcgcaat ccgcgtcacg cccgcgcgct gatcgcccgc ggcatcctgc tcgaggcggc
     4921 aggccgcacc ggcgaagcgc tggccgactt cgaccgcacc gtcgggctcg acccgaacaa
     4981 cccgacggcg ctctttcttc gcggtcggat ccgtgcggct cgcggtgagc gactcgcggc
     5041 gctcgccgat ttcgagaccg cgctcgccgt cgcaccgccc gactggaccg atcgtccgcg
     5101 ggtcgagcgc gcgctcgaac ggctgcggtc cggcccgcct ggcggcgaat agaccggcat
     5161 cgtcccgtca ggccgggttt tctcccggcc cgacgcggtg attccagcta agatggct
//
LOCUS       DASPGN010000173        14395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_730546, whole genome shotgun sequence.
ACCESSION   DASPGN010000173 DASPGN010000000
VERSION     DASPGN010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 14395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14395
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730546"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..786
                     /locus_tag="VD788_04930"
     CDS             121..786
                     /locus_tag="VD788_04930"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXV75643.1"
                     /translation="MNRKLFEAIDSGEIEPVREMIGRDPALAVARDERGVSAVGHARY
                     RMRPELVELLLAAGPELDLFDAAAVGRVERLRELLDGDPSLVSAVSGDGGSALHLAAF
                     LGSADAVRLLLERGADADAVAPGFNEVKPLHSAVASRRPEVVHLLLEHGCDVNAKQRG
                     GFNALHAAAHAGMTDVVDALLARGADPKATNDAGRTATDLAREAGHHAIAERLALATS
                     DDG"
     gene            1013..2410
                     /locus_tag="VD788_04935"
     CDS             1013..2410
                     /locus_tag="VD788_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75644.1"
                     /translation="MRMNAWIGAIVLLMLATPVWAGAVCTDISDQCLTTAAEDCDANT
                     QEVVVQIGGPGAEDSNGDGGTCQGTNATALLTFTKVDGDTYTVTVENTTCGDNGSVTA
                     VFHNTTDTVTAASEDALSALAGVVDVNWFLHYEQTTPTDGDNNNGPKADGFGHFDIVI
                     YNGDSASPNGGDKRDIRPGEKFRFEISLAPGYELCDFLTEISTPTPGDRNRVAVGRWQ
                     ACGADEQNSAYIGPCTPDGELLAVIDRIVLTPDDRRMRVEWSTSLEIENAGFNVLRRE
                     ATRGGWEQINVDLLPGRGDTVTGAEYEFVDETALNGVEYMYRIEDIDFGGRNNLSDIH
                     REVANPRNPRVKLVDPAYGATVALSNRTRFAFDGDAGNTILQISSDPTFSESSTVSTQ
                     VSRRFGGSEFTLDPRTSRIATSIARASDGIVYWRLVDRSRDAVSDTFRLEVEDDLVGS
                     VGHGAREISRAGGNR"
     gene            2407..2577
                     /locus_tag="VD788_04940"
     CDS             2407..2577
                     /locus_tag="VD788_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75645.1"
                     /translation="MSGDNQKRAKDNCEPTERRRAYQPPQLTSYDTKTIIQAIGPAYG
                     VYGAVPGMDTTL"
     gene            2715..6266
                     /locus_tag="VD788_04945"
     CDS             2715..6266
                     /locus_tag="VD788_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HXV75646.1"
                     /translation="MRVISTTRRLGVAAMSILAVGSSLTAIAAGSTDPQLLRSDDAAI
                     ELRVTFDPPRATALPAIPGSADLTVVGLPRHRVIGEPALPFRSVLVAVPPDAELRLEI
                     DDPRPTPIAGLRPAPFERQAGDDGPLAIATEPSRGRPFEAVEIDFDGYLRDRRVVRLL
                     FHPLTLDPAGLGTIYHPELTARLVAERRTGARVFVGRPGARVVGADGPSSLSGRPAER
                     ALYERTILNEHTIDDGAYRAVLRASRPEAGWSDLPSSSSLRGTGPLPAKIPVRGEDVW
                     SVTHAALLAAGFDASGLAVSALSLTSGCAPVPLHVVDGGDGTFDAGDRIEFYGVGISG
                     EIEYDVNVYRLAFDEGPGISPTTRDATPAGGTAPPSFTDTRRVEENLVFFTTRADSDG
                     DQIGWERVVASSAGNATASYQFTLPDVDPAAVTIRIRARIYDLGGSHTTSILLNGVDL
                     MDARSWSTGDVTHDATISAANLVPGTNTLTMQLSGSSFNQVVFNWFEVDYPRLYRAES
                     DRLAFTADLPAPVAHQVSGFTTSDIVVYDVTDPNAPARLDGVQISGFSGDYSAAFSDT
                     LGGERRFLARAGTSPPGGPWLVDQTSALGSTANGADLIVLAYRDFLDEMVPLVDARTA
                     QGWRVALVDLEDVFDEYACGRPDPMAIKSFLADAYLSWQAPSPAFLLLVGNATLDPLQ
                     QLPDSVPPLMPTGRFTAPTLGLAASDNALVTIVGDDPLPDLFVGRIPARTDEQAAAAV
                     AKIVAYDDVPPSRFGQGVLLAADNDDLAFEYIQEDLVTLYMSETRIPVERAYLRLLGA
                     GGTNQQIRSTINLGALTTNYLGHGNVHNWAAENVLVDSSDIPLLTNSDRPTFMTTLNC
                     INGFHAGPRGTSLDALAEQALVAPNGGAVAVWGPSALALLSDYTKISEVLYRQLFVDR
                     EQRLGVATTVAKVEAVTQFGTSVANLEEMHLFGDPTTVLRVDGDLDGLTDDEEDRNGL
                     AARDADSDDDGRDDGVEPLPPADSDGDGRTDAFDPDSDDDGLPDGLESGIVVPGPDTD
                     PTRGHFVPDADPLTMTDPADADTDGGGAPDGAEDRSADGQVGPGETDPLNPADDPGCA
                     TEPPAEVSGLLLAKSGDDATLSWSDQSASDPCVLYRVYVATDAAPSDGAAFLVEATTT
                     LAGWDHRGAVASDDLHFYLVTAITPIAGEGPRGHHGQ"
     gene            6306..6740
                     /locus_tag="VD788_04950"
     CDS             6306..6740
                     /locus_tag="VD788_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75647.1"
                     /translation="MKLPDEIRERFRCQGRDGGRRRARRMSASARRRVAREAATARWI
                     GHRFGSSSFGATGLPGGDLVDAGLADLAAGRETAASLVVSAAAPRLRREGVPLGAVFD
                     DPEIRLYRLLSASEGELAHARYNAYLRRLVSFADACRAARVD"
     gene            6783..7040
                     /locus_tag="VD788_04955"
     CDS             6783..7040
                     /locus_tag="VD788_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75648.1"
                     /translation="MMDELARTAPSYHYDPHSQMLSKIVRGFGKDLRDADRFVSSGMV
                     DPAEFRALVGGIPQAAYARYPSLTRAAVAAAVEQFFAERDR"
     gene            7111..8415
                     /locus_tag="VD788_04960"
     CDS             7111..8415
                     /locus_tag="VD788_04960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV75649.1"
                     /translation="MSQRSKFPPVFWVANLIEVLERFSYYGIYFGFGIYMTHLGFSRS
                     DLGLVQSVFLAVSYFVPVISGTMADKYGFKKVLIVSYVAYLPAILLLLVTESFSGIAI
                     TMLLIGMAAGIFKPLISGTVRAVTDGSNKTLGFGIFYAMVNVGGSFGPIVAGYLRAIS
                     WKYAFIASAVSIGVMLVITVLFYKEPPREADPASLGDKLVEILTVLKDLRFSSFLLIL
                     GVFFWLPFWAFFNLCAVYVDTNVDTAELYRNLDVVLGAIPLLGPWLVGVLSHEVDGTR
                     RVLGETISHTGYIIMVLQIFVSARIERWRAIPAFMLGLLIAAAGMVVLGFAVVAPAAL
                     VFAGIFLFAVGEMIASPRIQEYITWIAPKEKAGLYMGTNFIAVGIGGLLSGVIYTAVS
                     GAFIDVGHPEYVWYVLAAHLVVGIAVINLFTRVAGEFSERQA"
     gene            8580..9854
                     /locus_tag="VD788_04965"
     CDS             8580..9854
                     /locus_tag="VD788_04965"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV75650.1"
                     /translation="MIDSTTAMDRPVDNRRATARRRWLVGGAGSALLLAAAALYGPAS
                     RWMSADRSVDLSRIRVGTVVRGDLERDLSVQGRIVAAFHPTMFSPASGIVSLAVRAGE
                     VVAVGQVLAVVDSPELNSRLRQERATLSALESDLDRQRILASQERLANRQQIDLAEVE
                     LEAARRAMDRAERSREEGILNEVEYEEAQDELRRSELALDHAREDAELEHDTLEFEIR
                     NRELSLERQREVVADLERQVAELSIRAPVDGLVSRLDVEDHDAVTPGREIVTVVDLSA
                     FEIEVQIPEGYADDVGIGTAVEVHYDGEPYPAEVKVVSPEVEGSQVRGIVAFTGTPPE
                     GLRQNQRVSTRLILESRSDVLIVPRGPYLEVGGGREAFVIRGDTAELRPIRVGAVGVA
                     EVEIVDGLVEGDRIIISDTSRFTDARKVFLRQ"
     gene            9865..10593
                     /locus_tag="VD788_04970"
     CDS             9865..10593
                     /locus_tag="VD788_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV75651.1"
                     /translation="MLQMNQVRKVYRTDYVETHALSAFNLEVAEGDFVSVMGPSGSGK
                     TTFLNIAGLLDSFDGGRYLLDGADVSRLRDAEMSRIRNRKIGFIFQTFNLIPDLNVFD
                     NVDVPLRYRGLRAGARRRAIEEALAIVGLGSRLRHYPAQLSGGQQQRVAIARALVGQP
                     RLILADEPTGNLDSAMANEILDLLEKINSTGTTIVMVTHDPELANRATRQIHLLDGKL
                     IDLAADRPAPLASDGSDGARAGMA"
     gene            10602..11912
                     /locus_tag="VD788_04975"
     CDS             10602..11912
                     /locus_tag="VD788_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV75652.1"
                     /translation="MLVYQLRLAWLSLRRNPILSVLIVSGIGLGIGVAMTFVTAFYVV
                     SGDPIPHKSDRLFYVQLDSWDPQRPWDDDRPSEPPSQITYLDMVGIMRSDIPTYQGAM
                     HKTYLTVHPPEEGVRPFRETVRMCFGDFFPMFDVPFRYGSGWRDEEDDGPGAVIVLGD
                     AVNRKLFGGENSVGRTLRIEDREFRVVGVLERWRPTPKFYDTQNDEFGPVEEIFMPFR
                     WGQEMQIGTSGNTSSWRSAGSGYEALLRSETVWIQMWVQLDTAEQKREYQAFLDAYVT
                     EQKAAGRFQRPLNNRLRDVTEWLEAEEVMPDEARSLLTIALLFLLVCSVNLIGILLGK
                     FLARAPEIGVRRALGASRRWVFVQHLIECEVIGVAGGLLGLVLAVGGLELLDRLYDSQ
                     LNFRLDMNMFWVALALALLSAMIAGVYPAWRICRIVPGYYLKLQ"
     gene            11926..13176
                     /locus_tag="VD788_04980"
     CDS             11926..13176
                     /locus_tag="VD788_04980"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXV75653.1"
                     /translation="MQIGPIFRAMFRHKGRFWLIAVEVALTLAIISNCLNMVLVERQK
                     MNRPTGIDEDNILVVYSEPFDKAFEDKIYARASYDNDLRALRGMPGVHAAAGMHAIPL
                     SGGGSATGRRPRGSEIDTLSAPYFIVSEGVIAALGVELVEGRDFTAADFPTPDDDEAS
                     ATAEDEAAAEPDLSNVIVTREFAELMYPDGDALGKTIENRNGDSIDTIIGVIERMHNS
                     WPLASFAERVVLYPGEPWSERGARYVVRAEPGMLGELYTTIEDELIRQDEGRLIRVET
                     LAEVKGDTYSGTHAVTKMLGGLSVLLILVTSLGIIGLTSFSVTQRTREIGTRRALGAT
                     RTSILGYFLVENWIVTGVGLVVGVGLTYGLNFVLAQWAEVQRIEIPQVAVGVLTIWLA
                     GLAAALAPAIRGTTVQPVTATRNV"
     gene            13213..>14395
                     /locus_tag="VD788_04985"
     CDS             13213..>14395
                     /locus_tag="VD788_04985"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV75654.1"
                     /translation="MDAEPLRVLVVDDEPSILHALRILFEVHGIPARSATNPEDAMRA
                     IAAGDVGVIVQDMNFGADKTSGEQGVRLFRRIRQADPEVPVVLMTAWASLETAVTLIK
                     EGANDYLAKPWDDDKLVLTVRNLLRLRTLQLENERLQQQGRHARDLLAARYELCGLIY
                     ESEEMQHLVSLAVHVASSDAPVLITGPSGVGKEKLAEIVQANSRRRDRPFLRVNVGAL
                     PDELMESELFGAEAGAYTGSKGLRVGHFEAADGGTLFLDEIDALSLAGQIKLLRVVQS
                     GEFQRLGSSRTRRVDVRLISASNADLRRAIADGRFRDDLYFRLNVIELALPPLVDRPQ
                     DILPLARAFLARAAEARADAPSVFDAEAEAALICHDWGGNVRELENRIRRAGLTAAGG
                     SV"
BASE COUNT         2260 a         4878 c         4984 g         2273 t
ORIGIN      
        1 tcgtcgaggc ccgcgagcga aggtagcccc acgcgacgcg agcgttaccc gggagcccga
       61 ggagcgcttc gccggcgcgg gcgtgaacag tcgctcgagc gcatccgccg aggccgagcc
      121 atgaaccgga aactgttcga agcgatcgac tcgggtgaga tcgagcccgt gcgcgagatg
      181 atcggccgcg acccggccct cgcggtcgcg cgcgacgagc gcggcgtgtc ggcggtaggc
      241 cacgcccgct accgcatgcg tcccgaactc gtcgagctct tgctggccgc cggcccggag
      301 ctggacctgt tcgatgccgc ggcggtcgga cgcgtcgagc gtctgcgcga gctgctcgac
      361 ggcgacccct cgctcgtctc ggccgtctcg ggcgacggtg gcagcgcgct gcacctcgcc
      421 gctttcctcg gctcggccga cgcggtccgg ctgctgctcg agcgaggcgc ggacgccgat
      481 gccgtcgccc cgggcttcaa cgaggtgaaa ccgctgcaca gcgccgtcgc cagccggcgg
      541 ccggaggtcg tacacctgct gctcgagcac ggctgcgacg tcaacgccaa gcagcgcggc
      601 ggcttcaacg cgctccacgc cgcggcccat gccggcatga ccgacgtcgt cgacgcgctc
      661 ctcgcgcgcg gcgccgatcc gaaggcgacc aacgacgccg ggcggaccgc caccgatctc
      721 gcccgcgaag cgggccacca cgccatcgcc gaacgactcg cgctcgccac ctccgacgac
      781 ggttgactcg atcgatccgt ggccggccga aaccaccccg gcgatgccga cttcttacca
      841 atccactatc gccgccctcc gtcgctccgg cgtttcgccg aacactcgct tgaccgcgtc
      901 gaccgattct gtacaagtaa tgactagcaa ctggggtcac gagatcgttc tcagaccgac
      961 gaccgcgggt tgacgcgacg gcccagcgaa tgagcggggc gggagtaagc gaatgcgaat
     1021 gaacgcatgg atcggggcca tcgtgctgct gatgctggcg acgccggtgt gggcgggagc
     1081 ggtgtgcacc gacatttccg atcaatgtct gaccacggcc gcggaggatt gcgacgccaa
     1141 cacccaagag gtcgtcgtcc aaatcggcgg cccaggtgcc gaggacagca acggcgacgg
     1201 aggcacgtgc caaggcacga acgcgacggc gctcttgacc ttcaccaagg tcgacggcga
     1261 cacctacacg gtcaccgtcg agaacaccac ctgcggtgac aacggaagcg tcacggccgt
     1321 gttccacaac acgaccgata ccgtgaccgc tgcgagcgag gacgcgttgt ccgccttggc
     1381 cggcgtcgtc gatgtgaact ggttcttgca ctacgagcag acgacgccaa ccgacggcga
     1441 taacaacaac gggcccaagg ccgacggttt cggtcatttc gacatcgtga tctacaacgg
     1501 cgacagcgcg agcccgaacg gcggcgacaa gcgcgacatc cggccgggcg aaaagttccg
     1561 gttcgagatc tcgctggcac cgggttacga actatgcgac ttcctgaccg agatcagcac
     1621 gccgacgccg ggtgaccgca atcgcgtagc ggtcgggcgc tggcaggcct gtggtgccga
     1681 cgagcaaaac agcgcgtaca tcggtccgtg cacgccggac ggcgagctgc tggcggtcat
     1741 cgatcggatc gtgctgacgc cggacgatcg gcggatgcgc gtcgagtggt cgaccagcct
     1801 cgagatcgaa aacgccggct tcaacgtgct gcgccgggaa gcgacgcgcg gcggctggga
     1861 gcagatcaat gtcgatctgc ttcccggtcg aggtgacacg gtgaccggcg ccgagtacga
     1921 gttcgtcgac gagacggcac tcaatggcgt cgagtacatg taccgcatcg aggacatcga
     1981 cttcggcggc cgcaacaacc tgagcgacat ccaccgcgag gtggcgaacc cacggaaccc
     2041 acgggtcaag ctcgtcgatc ccgcctacgg cgcgacggtc gcgcttagca accggacccg
     2101 cttcgcgttc gacggagacg ccggcaacac gatcctgcag atctcgagcg acccgacttt
     2161 ctcggagtcg tcgacggtct cgacgcaggt gtcccggcgc ttcggcgggt ccgagttcac
     2221 gctcgacccg cggacgtctc ggatcgcaac gtcgatcgcg cgcgcgtcgg acggcatcgt
     2281 gtactggcgt ttggtcgacc ggtcccgcga cgcggtgagc gacacgttcc gtttggaggt
     2341 tgaggacgac ttggtcggct cggtcggcca cggagcgaga gagatctcgc gggcgggagg
     2401 caaccgatga gcggcgacaa ccagaagaga gcgaaggaca actgcgaacc aaccgagcgc
     2461 cggcgcgcct accagcctcc gcagctgacg tcctacgaca cgaagacgat catccaggcg
     2521 atcggcccgg cctacggagt ctacggcgcc gttcccggca tggacacgac cctgtaattt
     2581 cgccgggcga acagcgagga agacgcgaag tcgccgcccc tcgatcggtt ccgccgaacc
     2641 gaggaggcgg cgatttcgcg ttcgggtagg aagacacccc gcacgtgcgt atcggtaggc
     2701 gagggcccga agcgatgcgg gtaatttcga caactcggcg tctcggcgtc gcggcgatgt
     2761 cgattctcgc ggtcggatct tcgctcaccg cgatcgccgc cggttcgacc gatccgcagc
     2821 tgctgcgctc ggacgacgcc gcgatcgagt tgcgcgtcac cttcgacccc ccgcgcgcga
     2881 cggcactgcc tgccattccc ggatcggcgg atctcaccgt cgtcggcctt ccgcggcacc
     2941 gcgtcatcgg cgagcccgcg ctcccgtttc ggtcggtgct cgtcgccgtc ccgcccgacg
     3001 ccgagcttcg tctcgagatc gacgacccgc gtccgacgcc catcgccggt ctccgcccgg
     3061 cgccgttcga gcgacaggcg ggggacgacg gaccgctcgc gatcgcgacg gagccgtcac
     3121 gcgggcgccc gttcgaagcc gtcgagatcg acttcgacgg gtacctgcgc gaccgccggg
     3181 tcgtgcgctt gctgttccat ccgctgacgc tcgacccggc ggggctcggc acgatctatc
     3241 acccggagct gacggctcga ctggtcgccg agcggcggac aggcgcccgt gttttcgtcg
     3301 gacgacccgg cgcgcgggtg gtgggggccg acggaccaag ctcgctctcg ggccggcccg
     3361 ccgagcgcgc gctctacgaa cgaacgatcc tcaacgagca cacgatcgac gacggggcat
     3421 accgcgccgt gctgcgcgcc agccggccgg aggccgggtg gtccgacctc ccgtcgtcgt
     3481 catcgctccg cgggacgggc ccgctgccgg ccaagatccc cgttcgcggc gaggacgtgt
     3541 ggagcgtgac ccacgccgcg ctgctcgcag cgggcttcga tgcgagcggg ctcgccgtgt
     3601 cggcgctctc gctgacgagc ggctgcgcgc cggtgccgct ccacgtggtc gacggcggcg
     3661 acggtacgtt cgacgcgggc gaccgcatcg agttctacgg cgtcggcatc tcgggcgaga
     3721 tcgagtacga cgtcaacgtc tatcgcctgg cgttcgacga gggtcccggc atctcgccca
     3781 cgacgcgcga cgcgacgccg gccggaggca cggcgccgcc gtcgtttacc gacacgcgcc
     3841 gcgtcgagga gaacctggtc ttcttcacca cgcgcgccga cagcgacggc gaccagatcg
     3901 gctgggagcg tgtcgtggcg tcgagcgcag gaaacgcgac cgcgagctac cagttcacgc
     3961 tgcccgacgt cgacccggcg gcggtgacga ttcgcatccg tgctcggatc tacgacctcg
     4021 gcggctcgca cacgacctcg atcctgctca acggcgtcga tctgatggat gcgcggtcgt
     4081 ggagcacggg agacgtcacg cacgacgcga cgatctcggc cgcgaacctc gtgcccggga
     4141 cgaacacgct caccatgcag ctctccggct caagcttcaa tcaggtggtg ttcaattggt
     4201 tcgaggtcga ctacccgcga ctctaccgcg ccgagagcga tcgcctggcg ttcaccgcgg
     4261 acctgccggc gccggtggcc caccaggtct cgggcttcac gacatcggac atcgtggtct
     4321 acgacgtgac cgacccgaac gcgccggccc ggctcgacgg cgtccagatc tcgggcttct
     4381 ccggcgacta ctcggctgcc tttagcgaca cgctcggggg cgaacgtcgc ttcctcgcgc
     4441 gcgccggcac gagcccgccc ggtggtccct ggctcgtcga ccagacgtcg gcgctcggta
     4501 gcacggccaa cggtgccgac ttgatcgtgc tggcctaccg tgacttcctc gacgagatgg
     4561 tgccgctggt cgacgccagg acggcccagg gctggcgcgt cgcgctggtc gatctcgagg
     4621 acgtcttcga cgagtacgcc tgcgggcgtc ccgatccgat ggcgatcaag agcttcctcg
     4681 ccgacgccta cctctcgtgg caagcgccct ccccggcctt tctgctgctg gtgggcaacg
     4741 cgaccctcga tccgttgcag cagctgccgg acagcgtgcc gccgctgatg ccgaccggga
     4801 ggttcaccgc accgacgttg ggcctcgcgg cgagtgacaa cgcgctggtg acgatcgtcg
     4861 gtgacgatcc gttgccggac ctgttcgtcg gccgcatccc ggcgcgcacc gacgagcagg
     4921 cggccgccgc ggtggcgaag atcgtcgcct acgacgacgt gccgccgtcg cgcttcggtc
     4981 agggcgtgct gctcgccgcc gacaacgacg acctggcgtt cgagtacatc caggaagacc
     5041 tggtgacgct gtacatgtcg gaaacgcgga tcccggtcga gcgggcttat ctgcggctgc
     5101 tcggcgccgg cggcaccaac cagcagatcc gctcgacgat caacctgggc gcgttgacca
     5161 cgaactacct cggtcacggc aacgtgcaca actgggccgc ggagaacgtc ctcgtcgatt
     5221 cgtcggacat tccgctactg acgaactccg accgcccgac cttcatgacg acgctgaact
     5281 gcatcaacgg gtttcacgcc ggcccgcgtg gcacctcgct cgatgcgctg gccgagcagg
     5341 cgctcgtcgc tccgaacggg ggcgcggtcg cggtctgggg gccgtcggcg ctggcgttgt
     5401 tgagcgacta tacgaagatc tccgaggtgc tttaccgcca gttgttcgtc gaccgtgagc
     5461 agcgactcgg cgtggcgacg acggtggcca aggtcgaagc cgtcacccag ttcgggacgt
     5521 ccgtggccaa cctcgaagag atgcacctgt tcggggatcc gacgacggtg ctgcgcgtcg
     5581 acggcgacct cgacggtctg acggacgacg aagaagatcg gaacggcctc gcggcgcgcg
     5641 acgccgatag cgacgacgac ggtcgggacg acggcgtcga gccgctgccg ccggccgact
     5701 ccgacggcga cggccgcacc gacgcgttcg atcccgactc cgacgacgac ggtcttccgg
     5761 acggactcga gagcggaatc gtcgtgccgg gaccggacac cgatccgacg cgcggtcact
     5821 tcgtgccgga cgccgatccg ttgacgatga ccgatcccgc cgatgccgac accgacggag
     5881 gcggcgcccc ggacggagcg gaggaccgca gcgccgacgg tcaggtcggg cccggggaga
     5941 ccgacccgct gaaccccgcg gacgatcccg gctgcgcgac cgaacccccg gccgaggtct
     6001 cggggctgct gctcgcgaag agcggcgacg atgcgacgct ctcgtggagc gaccagagcg
     6061 cgagcgaccc gtgtgtgctc tatcgggtct acgtcgcgac ggacgcggca ccgtcggacg
     6121 gagccgcgtt cctcgtcgag gcgacgacga cgctcgcggg ttgggatcat cgcggcgccg
     6181 tcgccagcga cgacctccac ttctacctcg tcaccgcgat cacgccgatc gccggcgagg
     6241 gcccgcgagg ccaccacggc cagtagatca gaatgcttac ccccttggca tatcgtggtc
     6301 ggaggatgaa gctccccgac gagatccgcg agcggttccg gtgtcagggt cgggacggtg
     6361 gacgccggcg cgcccgccgg atgtccgctt cggcccgacg ccgcgtggcc cgggaggcgg
     6421 cgaccgcgcg ctggatcgga caccggttcg gttcgtcgag cttcggggcg acgggcctcc
     6481 ccggtggcga tctggtcgac gccgggttgg ccgacctcgc cgccggccgc gaaacggcgg
     6541 cgagcctcgt cgtctcggcg gcggcgccgc ggctgcgtcg cgagggggtt ccgctcggcg
     6601 cggtcttcga cgatccggag atccgcctgt atcggctgct gtcggcgagc gagggcgagt
     6661 tggcccacgc gcgatacaac gcctacctcc gccggctggt ctcgttcgcc gacgcttgtc
     6721 gggcggcccg cgtcgactga gcccccgtgc gccgtgagct gactcgcgcg aggttccgcg
     6781 agttgatgga tgaactcgcc cggaccgcac cgtcttacca ctacgatccg cactcccaga
     6841 tgttatcgaa gatcgtgcgc gggttcggca aggacctgcg cgacgccgat cgattcgtca
     6901 gcagcggcat ggtcgacccg gcggagtttc gagcgctggt cggcgggatc ccgcaggccg
     6961 cctacgcgcg ctacccgagc ctcacgcgcg cggccgtcgc cgccgcggtc gagcagttct
     7021 tcgccgagcg cgatcggtag cccgcatctc gtactacaat cgcccgccgg tcgccgctcg
     7081 cgtcgcccgc ggcgcggaaa ggagagcgag ttgagccaga gatccaagtt cccgccggtc
     7141 ttctgggtcg ccaacctgat cgaggtgctc gagcgtttct cgtactacgg catctacttc
     7201 ggcttcggga tctacatgac ccacctcggc ttttcgcggt ccgacctggg tctggtgcag
     7261 agcgtgttcc tcgccgtgtc gtacttcgtc ccggtgatct cgggcacgat ggccgacaag
     7321 tacggtttca agaaggtgct gatcgtcagc tacgtggcct acctgccggc gatcctgctg
     7381 ttgctcgtga ccgagtcgtt ctccggcatc gcgatcacga tgctgttgat cggcatggcg
     7441 gccggcatct tcaagccgct gatctccggc acggtgcgtg cggtgaccga cggcagcaac
     7501 aagaccctgg gcttcggcat cttctacgcg atggtcaacg tcggcggctc gttcgggccg
     7561 atcgtcgccg gctatctccg tgcgatctcg tggaagtacg cgttcatcgc gtcggcggtc
     7621 tcgatcggcg tgatgctggt gatcaccgtg ttgttctaca aggagccgcc gcgcgaggcc
     7681 gacccggcgt ccctcggcga caagctggtc gagatcctca ccgtgttgaa ggatctgcgc
     7741 ttctccagct ttctgttgat cctcggcgtg ttcttctggc ttccgttctg ggcgttcttc
     7801 aatctgtgtg cggtctacgt cgacaccaac gtcgacaccg cggagctgta ccgcaatctc
     7861 gacgtggtgc tcggcgcgat cccgctgctc ggcccgtggc tggtcggcgt gctgtcgcac
     7921 gaggtcgacg ggacgcgccg cgtgctgggc gagacgatct cccacacggg ctacatcatc
     7981 atggtgctgc agatcttcgt ctcggcgcgc atcgagcgct ggcgcgcgat cccggcgttc
     8041 atgctcgggc tgttgatcgc cgccgccggg atggtcgtgc tcggcttcgc ggtggtcgcg
     8101 ccggcggcgc tggtcttcgc cggcatcttc ctgttcgccg tcggcgagat gatcgcttcg
     8161 ccgcggatcc aggagtacat cacctggatc gcgcccaagg agaaggccgg cctgtacatg
     8221 ggcaccaatt tcatcgcggt cggcatcggc gggttgctca gcggcgtgat ctacaccgcg
     8281 gtctccggcg cgttcatcga cgtcggtcat cccgagtacg tgtggtacgt gctggccgca
     8341 cacctggtcg tcggcatcgc ggtgatcaac ctgttcaccc gcgtggccgg ggagttcagc
     8401 gagcgccagg cctgagacct accggacagc tgtccgcgcc gacgccggac agctgtccgt
     8461 gtgacccgcc cggcggggtg cggttgcggc ccggttccgg ccgcttgtcg cccgtccgag
     8521 ccgcccgcga ggctggcaac gaccttgcag gggcgaccgc cgagggggac ggctcggcga
     8581 tgatcgacag taccaccgcg atggatcggc cggtcgacaa ccgccgggcg accgcgcgcc
     8641 gccgctggtt ggtcggcgga gcgggatcgg cgctgctgct cgcggcggcc gcgctgtacg
     8701 ggccggccag ccgctggatg agcgccgatc gctcggtcga cctgtcgcgc atccgcgtcg
     8761 gcaccgtcgt gcgcggcgac ctggaacgcg acctgtcggt ccagggccgc atcgtcgccg
     8821 cgtttcaccc gacgatgttc agcccggcga gcggcatcgt ctcgctggcg gtgcgcgccg
     8881 gcgaggtcgt cgcggtcggc caggtgctgg ccgtcgtcga cagcccggag ctgaacagcc
     8941 ggctgaggca ggagcgtgcg acgctctccg cactcgagtc cgacctcgat cgccagcgga
     9001 tcctggccag ccaggagcgc ctggccaacc gccagcagat cgacctggcc gaggtcgagc
     9061 tcgaggccgc gcggcgcgcg atggaccgcg ccgagcggtc gcgcgaagag ggcatcctca
     9121 acgaagtcga atacgaggag gcccaggacg agctgcgtcg atcggagctc gcgctcgatc
     9181 acgcgcgcga ggacgccgag ctcgagcacg acacgctcga attcgagata cgcaatcgtg
     9241 agctgtcgct agaacgccag cgcgaagtcg tcgccgatct cgagcgccaa gtggccgagc
     9301 tgtcgatccg cgccccggtc gacgggctgg tgtcgcgcct cgacgtcgag gaccacgacg
     9361 cagtgacgcc cggtcgcgag atcgtgacgg tcgtcgacct gtcggcgttc gagatcgagg
     9421 tccagattcc ggaaggctac gcagacgacg tcggcatcgg caccgcggtc gaggtgcact
     9481 acgacggcga gccgtacccg gccgaggtca aggtggtctc gccggaggtc gagggcagcc
     9541 aggtgcgcgg gatcgtcgcc ttcaccggca cgccgcccga agggttgcga cagaaccagc
     9601 gcgtgtcgac gcggttgatc ctcgagtctc gcagcgacgt gttgatcgtg ccgcgcggac
     9661 cgtatctcga ggtcggcggc ggccgcgagg cgttcgtgat ccgcggggac acggcggagc
     9721 tgcgcccgat ccgcgtcggc gcggtcggcg tggccgaggt cgagattgtc gacgggctgg
     9781 tcgaagggga ccgcatcatc atctcggaca cttcgagatt caccgacgcg aggaaggttt
     9841 ttcttcgtca gtagggagag cgacatgcta cagatgaacc aggtgcgcaa ggtctatcgg
     9901 accgactacg tcgagaccca cgcgttgagc gccttcaacc tggaggtggc ggaaggcgat
     9961 ttcgtctcgg tgatgggtcc gtcggggtcg ggcaagacga ccttcctcaa catcgccggc
    10021 ctgctcgact cgttcgacgg cgggcgctac ctgctcgacg gagcagacgt cagccggctg
    10081 cgcgacgccg agatgtcgcg gatccgcaat cgcaagatcg gcttcatctt ccagacgttc
    10141 aacttgatac ccgatctcaa cgtgttcgac aacgtcgacg tgccgctgcg ctatcgcggg
    10201 ttgcgggccg gcgcgcgccg ccgggcgatc gaggaggcgc tggcgatcgt cggtctcggc
    10261 tcgcgcctgc gccactatcc ggcgcagctc tcgggtggcc agcagcagcg agtggcgatc
    10321 gcgcgggcgc tggtcggtca gccgcggctg atcctcgccg acgagccgac cgggaatctc
    10381 gactcggcga tggccaacga gatcctcgat ctgctggaga agatcaactc gacggggacg
    10441 acgatcgtga tggtgaccca cgacccggag ctcgccaacc gcgccacccg tcagatccac
    10501 ctgctcgacg gcaagctgat cgacctcgcc gcggaccggc cggcgccgct cgcgagcgac
    10561 gggtcggacg gcgcgcgggc cggaatggcg tgaggacgga aatgctcgtc taccagttga
    10621 gactcgcctg gctcagcctc cgtcgcaatc cgatcctgtc ggtgctgatc gtcagcggca
    10681 tcggtctcgg catcggcgtc gcgatgacct tcgtcaccgc gttctacgtc gtctcgggcg
    10741 acccgatccc gcacaagagc gaccgcctgt tctacgtcca gctcgactcg tgggaccccc
    10801 agcggccgtg ggacgacgac cggccgagcg agcctccgag tcagatcacg tacctcgaca
    10861 tggtcggcat catgcggtcc gacatcccga cgtatcaagg tgcaatgcac aagacctacc
    10921 tgaccgtcca tccgcccgag gagggcgtgc gcccgttccg cgagaccgtg cggatgtgct
    10981 tcggcgattt cttcccgatg ttcgacgtgc cgttccgcta cggatcgggc tggcgcgacg
    11041 aggaggacga cggaccgggg gcggtgatcg tactgggcga cgcggtaaac cggaagctgt
    11101 tcggcggaga gaacagcgtc ggacggacgc tgcggatcga ggaccgggag ttccgcgtcg
    11161 tcggcgtgct cgagcgctgg cgcccgacac cgaagttcta cgacacgcag aacgacgagt
    11221 tcggtccggt cgaagagatc ttcatgccgt tccgctgggg acaagagatg cagatcggca
    11281 cctcgggcaa cacctcgagc tggcgcagcg ccggttcggg ctacgaggcg ctgttgcggt
    11341 cggagacggt gtggatccag atgtgggtcc agctcgacac cgccgagcag aagcgcgagt
    11401 accaggcgtt cctcgacgcc tacgtcaccg agcagaaggc ggccggccgc ttccagcgcc
    11461 cgctcaacaa tcggctgcgc gacgtgacgg aatggctcga ggcggaggag gtgatgccgg
    11521 acgaggcgcg ctcgttgctg acgatcgcgc tgctgttcct gctcgtctgc tcggtcaacc
    11581 tgatcggcat cctgctcggc aagttcctgg cgcgtgcccc ggagatcggc gtgcggcgtg
    11641 cgctcggcgc cagccggcgc tgggtgttcg tccagcactt gatcgagtgc gaggtgatcg
    11701 gcgtcgccgg cggtctgctc ggcttggtgc tcgcggtcgg cgggctcgag ctgctggacc
    11761 ggctgtacga cagccagctg aacttccggc tggacatgaa catgttctgg gtcgcactcg
    11821 cgctcgcgct gctgtcggcg atgatcgccg gcgtttaccc ggcgtggcgt atctgtcgca
    11881 tcgtgccggg ctactacctg aagctccagt gaaaggagtg ccgtgatgca gatcggaccg
    11941 atctttcgcg cgatgttccg ccacaagggc cgcttctggt tgatcgccgt cgaggtcgcg
    12001 ctgacgctcg cgatcatcag caactgcctc aacatggtcc tcgtcgagcg gcagaagatg
    12061 aaccgtccga ccgggatcga cgaagacaac atcctcgtcg tctacagcga gccgttcgac
    12121 aaggcgttcg aggacaagat ctacgcgcgc gcgagctacg acaacgatct gcgcgcgctg
    12181 cgtggcatgc ccggcgtgca cgccgccgcc gggatgcacg ccatcccgct gagcggcggc
    12241 ggcagcgcga cggggcggcg accgcgcggt agcgagatcg acacgctgtc cgccccgtac
    12301 ttcatcgtca gcgagggcgt gatcgcggcg ctcggcgtcg agttggtcga gggtcgcgat
    12361 ttcaccgccg ccgatttccc cacgcccgac gacgacgagg catcggcgac ggccgaggac
    12421 gaagcggccg ccgagcccga tctgtcgaac gtgatcgtca cacgcgaatt cgccgagctg
    12481 atgtatcccg acggcgacgc actgggcaag acgatcgaga accgcaacgg cgacagcatc
    12541 gacacgatca tcggcgtcat cgagcgcatg cacaactcgt ggccgctggc gagcttcgcg
    12601 gagcgcgtcg tgctgtatcc cggcgagccg tggagcgagc ggggcgcgcg ctacgtcgtc
    12661 cgcgccgagc ccgggatgct gggtgagctg tacacgacga tcgaagacga gctgatccgg
    12721 caggacgagg gccgattgat ccgtgtcgag acgctggccg aggtcaaggg cgacacctac
    12781 agcggaacgc acgcggtgac caagatgctc ggcgggctga gcgtgctgct gattctggtt
    12841 acgtcgctcg gcatcatcgg cctgacgtcg ttctcggtca cgcaacgcac gcgcgagatc
    12901 gggacgcggc gggcactcgg tgcgacgcgg acgtcgatcc tgggttactt cctcgtcgag
    12961 aactggatcg tcaccggggt cgggctggtc gtcggggtcg ggctgaccta cggactgaac
    13021 ttcgtcctcg cgcagtgggc cgaggtccaa cggatcgaga taccgcaggt cgccgtcggc
    13081 gtgctgacga tctggctcgc cggtctggcc gccgcgctgg cgccggcgat acgcggcacg
    13141 accgtgcagc cggtgaccgc cacgcgcaac gtctgacgcc ggttccggcc ggcgcgcgga
    13201 tagaatggaa cgatggacgc cgagccgctg agagtactgg tcgtcgacga cgaaccgtcg
    13261 atcctgcacg cgctgcggat cctgttcgag gtccacggca tcccggcgcg cagcgcgacc
    13321 aacccggaag acgcgatgcg cgcgatcgcc gcgggcgacg tcggcgtcat cgtgcaggac
    13381 atgaacttcg gcgccgacaa gacgtcgggc gagcagggcg tccgtctgtt ccgtcgcatc
    13441 cgccaagccg atcccgaggt gccggtcgtg ctgatgaccg cgtgggcctc gctcgagacg
    13501 gcggtgacgc tgatcaagga gggggccaac gattacctgg ccaagccgtg ggacgacgac
    13561 aagctggtgc tgacggtgcg gaacctgttg cggctgcgca cgctccagct cgaaaacgag
    13621 cgactgcagc aacagggccg gcacgcacga gacttgctcg ccgcccgtta cgagctgtgc
    13681 ggcctgatct acgagagcga ggagatgcag cacctggtgt cgctcgcggt ccacgtcgcc
    13741 tcgtccgacg ccccggtgct gatcaccgga ccgagcggcg tcggcaagga aaagctggcc
    13801 gagatcgtcc aggccaattc tcgccgccgg gatcggccgt tcctccgcgt caacgtcggt
    13861 gcgctccccg atgagctgat ggagagcgag ctgttcggcg cggaagcggg cgcctacacc
    13921 gggtcgaagg gtttgcgggt cggccatttc gaagcggccg acggcgggac gctgttcttg
    13981 gacgagatcg acgcgctgtc gctcgccggg cagataaagc tgctgcgcgt cgtgcagagc
    14041 ggcgagttcc aacggctcgg cagcagcagg acccgccgcg tggacgtgcg cctgatcagt
    14101 gccagcaacg ccgatctcag gcgggcgatc gccgacggtc gcttccgcga cgatctctac
    14161 ttccggctca acgtgatcga gcttgcgctg ccgccgctcg tcgaccggcc gcaggacatc
    14221 ctgccgctgg cccgggcctt cctcgcgcgc gcggcggagg cacgcgccga cgcgccgtcg
    14281 gtcttcgatg ccgaggcgga ggcggcactg atctgtcacg actggggcgg caacgtccgc
    14341 gagttggaga accggatccg gcgggccggc ctgaccgcgg ccggcggctc ggtca
//
LOCUS       DASPGN010000174        11648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1433070, whole genome shotgun sequence.
ACCESSION   DASPGN010000174 DASPGN010000000
VERSION     DASPGN010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11648
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433070"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VD788_04990"
     CDS             <1..480
                     /locus_tag="VD788_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXV75655.1"
                     /translation="GEMRDLETIEIALETAETGHLVFGTLHTTTAASTVDRIVDQFPP
                     DRQSQIRVMLAESLKGVVSQMLLKRNGGGRVAALEILIVTTAISNLIREAKTFQIPSL
                     MQTGKKHGMRLLNDALLELVRGGSVDPHEAYRWSIDKTGLVTQLRAAGIALKHGPES"
     gene            complement(830..1285)
                     /locus_tag="VD788_04995"
     CDS             complement(830..1285)
                     /locus_tag="VD788_04995"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HXV75656.1"
                     /translation="MSRRVDWHTYFMNIAHQAATRATCDRKHVGAVIVRDKTILSTGY
                     NGSIRGMPHCDDVGHVMDNDHCVATVHAEANAIIQAAKNGVRIDGAEIYTTASPCWNC
                     FKLIANAGIRAIYYGEFYRDSRSVEVAARIGIRLYDLTRAVEQLESGED"
     gene            complement(1371..2762)
                     /locus_tag="VD788_05000"
     CDS             complement(1371..2762)
                     /locus_tag="VD788_05000"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV75657.1"
                     /translation="MKKILVVEDEPTLRDGIVTAFRDRGWHVAEAADGSEAFSRLESE
                     VFDVMVTDYKMPEHSGLDVLKRCKMINESTVVLMMTAYGTVDSAVEALKAGAYDYVLK
                     PFDLDELEMKVDKAMDHRRLLARVEAYDRDSIVAVFENIVGESPQMKEVFNTIRKVAR
                     SNATVMVLGETGVGKELVAEALHRNSNRSEHPFVKMNCAALHENLLESELFGHERGAF
                     TGADRQRIGRFELANGGTLFLDEIGNMSVSTQVKVLRVLQEREFERLGGSRTIKVDVR
                     LIAATNLDLEEAIAKGGFREDLYYRLNVVSIKVPPLRERREDIIPLAKIFMDESAQDI
                     KKDLRGIDPGAVRLLQRHTWPGNIRELKNVVERAVLLCETPYIRQEDLHLASNGSGVQ
                     EGGAPINLRLPPNGIELEELERLAILEALRINNWVQKDAARFLGISSRVMNYKVAKYE
                     IKNPRWSKNKLVS"
     gene            complement(2769..3941)
                     /locus_tag="VD788_05005"
     CDS             complement(2769..3941)
                     /locus_tag="VD788_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV75658.1"
                     /translation="MDDAATSVGPSLGLLRGIVDGMRCGILAVDRDGQLLMVNRIALQ
                     ILELDAAPPVATPVADALREFPLVTRVLGESFDMTSLPNRAELDLRPGDPRSKTIGFT
                     LSIIPGEPGERDPVGTAMFFKDLTQIEHKEEQDRLKDRLAALGQMAASLAHEIRNPLA
                     AIEVTCKLLQRRLASQNGGDEKSFDLLTKITGEVRRLNGTITSSLEFVRPLELRTAHA
                     ELNALLDEALHVARQRAGKPGIDVRCDFATDMPRFLMDPRQLRQVFENLLINAMEAID
                     GRGVIRVETEMRPAPSATSIPYPMGDDRGNDPWNRFEHFAVVRIADSGPGIGSEERDR
                     VFYPFFTTKKHGSGVGLSMAKKIVSSHHGLIDVADAPEGGALFTVRLPMVLDRAEV"
     gene            complement(4130..4726)
                     /locus_tag="VD788_05010"
     CDS             complement(4130..4726)
                     /locus_tag="VD788_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HXV75659.1"
                     /translation="MIHQHNERTRCPDPTRLLPLLLVTSLVACGGGGETSGLSGGPGG
                     GSGILAGNFTPDNPDPGANTVSIHGSSNTNIVTLNVTVTGVTDVFGASFDVTYDPTMT
                     EFVNWSPGSLLESNNQQVSYQINSQQAGRLVVGVARTTPSGGANAGISTGLVKLLFRV
                     TQAGASQVRFERAGLLNSADPPQSIGGIVFAGGTLTAN"
     gene            complement(4792..5652)
                     /locus_tag="VD788_05015"
     CDS             complement(4792..5652)
                     /locus_tag="VD788_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75660.1"
                     /translation="MWSQLLRRLALAGLLAALPGAASAGSIHLAWDAVPGATGYRAYF
                     GTASGQYTGVVDMKNATSGTLTGLLDCTTYYVSIKAYDTNGGISSQFSNEVAGWARPL
                     IDRMTPTSVVQGETRLTVDVFGANFDPDAALAWSIDGVPRDIDGNELIVLEDLAVQSC
                     TELRAYVTLEPAARGLRAMQIGGFNVDFEVVNPDTVFGTGRETIQVELDEIRADINQS
                     DGDTFERVDGKDLTWLARAHASREGQPYFNPDADLDGDGMVDGSDLAMLAARFGRCWS
                     GASWSDAACN"
     gene            6238..6843
                     /locus_tag="VD788_05020"
     CDS             6238..6843
                     /locus_tag="VD788_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75661.1"
                     /translation="MRTAWGALGLAVLAVACGVAHAGEVNLSWDPSSGASGYRVFSGS
                     SSGSYGSPQDVGSATTVPITTVPDCTTTYFAVTAYNGAGESGFSQEVSSWPRPVLSSI
                     NDPDAQRGESVTLVIGGANFQPGSSVAIPATGVVLNSATIDSCNQLTLGVSVQATAPF
                     GPVEILVTNGSGVEGRTSALFSVVGNPLPGVENLRRTDTTN"
     gene            6929..9220
                     /locus_tag="VD788_05025"
     CDS             6929..9220
                     /locus_tag="VD788_05025"
                     /inference="COORDINATES: protein motif:HMM:NF017280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HXV75662.1"
                     /translation="MLDRLRRSGLGDAARRWLPPPLRAVLHRLRGALEASSDPFRTCE
                     IALGRNLREESARSVDRWRTTGGVDDWRRLRDSFLTELGGSLGPWRPDERPLHSQTTG
                     RVEGDGFRVDCLVFTARPDVRITANLYRPAECDSPRPGIVFCHGHHAGKCQPELWEAG
                     VEWARAGCVVLIPDLAGHGERREHPFASPADHPGPFRPDRQDYWFRYSLGLQLAAVGH
                     SLLGWMVHDTLRSVDLLVARNDVDPARIAVVGSVAGGGDVAAVAAALDPRIDVVVPFN
                     FGGAMDDPGAGGWDASRSPRRRASHGLAPWMILASVAPRRLVYAHEFGWEAATDPVWE
                     RLRRTWRIFDAESRLAVVHGRGRISGRPPHHTHCTSVGRIHRERLAPTLARWLGVACP
                     QPGAPPMPHDRLHCLRDGDVKDGLVPIHRTVERSVIDRLTESRAARHRLAPADGRIAV
                     ALALSARLGPLDEPTAARFARVSRVGAAARGVERHELRRPGGARVRFDLRLPRDASSA
                     PFPLVVGLGQAPAHRLARRRRALIERLCGRGIAVALAQTRGSGPTRYDDAGYGRTSRA
                     TVVSASAWMLDRMWIAARLSDVAEVMRALAERPEIDARRVAVWGEGDAPPNADGSATS
                     GPIGVEPEPVVASPVGGIVALAWAVGHGSVRAVYVHRGLVSLASILESPRVCLPHEAV
                     IPGLLESLDLPDLAAALAPLPIRLEALVDGRNRPVDGDRLERAYACARQRYRDLGEDD
                     RLVLRPLDAADERVADWLGEALV"
     gene            complement(9227..10135)
                     /locus_tag="VD788_05030"
     CDS             complement(9227..10135)
                     /locus_tag="VD788_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HprK-related kinase A"
                     /protein_id="HXV75663.1"
                     /translation="MTVGELARADARGRAAGRGLAWEVGPFAVRLRTKLAAAVDGVRL
                     LYADFPLCDNGTVVDAEFIVRRKRFWPVAVTLRVDGEIQYDWLSPRLVVPMLEWAMNV
                     SVFQRVHQYLMLHSAVVARNGRAAILVGRAGSGKSTLCAGLVERGWRLLSDEVALVDP
                     SDGRLRAVPRPISLKGRSIEVIRRFAPGATIGPSWAGTAKGTVAHVVPPRDSVARSAE
                     TAEPAWVIFPIYADGEDASMAPLARAQAFVRAVDNAFNYGVYGRSGFELLSAMIGRCP
                     CFELCYGDLGRAVERIERLVADGGAV"
     gene            complement(10132..10395)
                     /locus_tag="VD788_05035"
     CDS             complement(10132..10395)
                     /locus_tag="VD788_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HXV75664.1"
                     /translation="MGRDGLVWRSWDPEQSIVYHCDSGDTHLVNSVTAEVLRVLERSP
                     CTASRIARTIAPRLGVGPDEGLARQVRELLEHLDRVGLAEPVG"
     gene            complement(10489..10734)
                     /locus_tag="VD788_05040"
     CDS             complement(10489..10734)
                     /locus_tag="VD788_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75665.1"
                     /translation="MPTILTLHAAPAWASTDYTLTAYRYGANAGRCRNPSYDPNADPS
                     STAGQEFIDCPLGTGSGQPTHEVDTTGQTDTRDFLRM"
     gene            11138..>11648
                     /locus_tag="VD788_05045"
     CDS             11138..>11648
                     /locus_tag="VD788_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV75666.1"
                     /translation="MDDLRAHFLEKYGPPEATGWGPRARFAFDYFTPADHYEAYVGRR
                     VDERTRWLDVGCGRALFSSDTIARRLSDRCGLLVGLDPDDNLDENPYVHEKVKASLYD
                     YRTERAFDLVTFNMVVEHITDPGEVVRVLSKLTAPGGEVVILTVFKWSPIPIATRLIP
                     FRFHHGFKHM"
BASE COUNT         1805 a         3954 c         3917 g         1972 t
ORIGIN      
        1 ggtgagatgc gcgacctcga gacgatcgag atcgcgctcg agacggccga gacggggcac
       61 ctcgtgttcg gcacgctgca cacgaccacg gcggcgtcga ccgtcgaccg catcgtcgac
      121 cagtttcccc cggaccggca gtcgcagatc cgcgtgatgc tcgccgagtc cctcaaaggc
      181 gtcgtctcgc agatgctgct caagcgcaac ggtggcggac gggtggccgc gctcgagatc
      241 ctgatcgtca cgaccgcgat ctcgaatctg atccgagagg ccaagacctt ccagatcccc
      301 agtctgatgc agaccggcaa gaagcacggg atgcgtttgc tgaacgacgc gttgctcgag
      361 ctcgtccgag gcgggtcggt cgatccccac gaagcctacc gctggtcgat cgacaagacc
      421 ggcctggtca cgcagctgcg agccgcaggc atcgcgctga agcacggccc ggagagctga
      481 tcgccggtcg cccccccaca gaaaaccagt tgactcgaag aaagaaccct atatattggg
      541 cgggatgttg agttatactc aacatgttga ggtaggtagc aatacctacg agcacagtcc
      601 atccacggaa ggaggtgatc ccgagatggc agccagaaag aaggccacca agaagaaggc
      661 caccaagaaa aaggccggac gaaaggccgg gaagaagaag gccaccaaga agcgggccag
      721 gcggaaggcc gctaagaaga aatagcgccg ccgcaataga tacctggaaa actgacaggg
      781 ccggacggga tcgccccgcc ggcccttttc ttcgacgggc gcggccgcgt cagtcctcgc
      841 ccgattccag ctgttccacc gcccgagtca ggtcgtaaag acggatgccg atccgcgcgg
      901 cgacctcgac gctgcggctg tcgcggtaga actcgccgta gtagatcgct cgaatgccgg
      961 cgttcgcgat cagcttgaag cagttccaac agggtgaggc ggtcgtgtag atctccgccc
     1021 cgtcgatccg cactccgttc ttggcggcct ggatgatcgc gttcgcctcg gcatggacgg
     1081 tggcgacgca gtggtcgttg tccatcacgt ggccgacgtc gtcgcagtgc ggcatgcccc
     1141 ggatcgagcc gttgtagccg gtcgacagga tcgttttgtc acgaacgatg accgcgccga
     1201 cgtgcttccg gtcgcaggtc gcgcgagtcg cggcctggtg ggcgatgttc atgaagtacg
     1261 tgtgccagtc gacccgtcgg ctcatcgcgc gctcccgtcg gccgctcgcc ggtccgcgcc
     1321 gaggacgggc agcgtagcat cgggagcgtg gggccggcca ggtccccggg ctagctgacc
     1381 agcttgttct tgctccagcg cgggttcttg atctcgtact tggcgacctt gtagttcatc
     1441 acgcgcgacg agatgccgag gaaccgtgcc gcgtctttct ggacccagtt gttgatgcgc
     1501 aacgcctcga ggatcgccag acgctccagc tcttcgagct cgatcccgtt gggcggcaat
     1561 ctcagattga tcggcgcgcc accctcctgg actcccgagc cgttgctcgc caggtgcagg
     1621 tcttcctggc ggatgtacgg ggtctcgcac agcagtacgg cgcgctcgac gacgttcttc
     1681 agctcacgga tgttgccggg ccaggtgtgg cgctgcagca gccgcaccgc acccggatcg
     1741 atcccacgca agtctttctt gatgtcctgt gccgactcgt ccatgaagat cttggccaac
     1801 gggatgatgt cctcgcgtcg ttcgcgcagc ggcgggacct tgatcgaaac cacgttcagc
     1861 cggtagtaca gatcctcgcg gaacccgccc ttggcgatcg cctcctccag gtcgaggttg
     1921 gtcgccgcga tcagtcggac gtcgaccttg atcgtgcgcg agcctcccag gcgttcgaac
     1981 tcgcgctcct gcagcacgcg cagcaccttg acctgggtcg agacgctcat gttgccgatc
     2041 tcgtcgagga acaacgtgcc gccgttggcc agctcgaagc ggccgatccg ttgtcggtcg
     2101 gcgccggtga acgcaccccg ctcgtgtccg aacagctccg actcgagcag attctcgtgc
     2161 agcgcggcgc agttcatctt gacgaacggg tgctccgatc ggttcgagtt gcgatgcaac
     2221 gcctcggcga cgagctcctt gcccacgccg gtctcgccga gcaccatcac ggtcgcgttg
     2281 ctgcgcgcga ccttgcggat cgtgttgaac acctctttca tttgcgggct ctcgccgacg
     2341 atgttctcga agaccgccac gatcgaatcg cgatcgtagg cctcgacgcg cgccaacagg
     2401 cgccggtggt ccatcgcctt gtcgaccttc atctcgagct cgtcgaggtc gaacggcttc
     2461 agcacgtaat cgtaggcccc ggccttcagc gcctcgacgg ccgagtcgac cgtcccgtag
     2521 gccgtcatca tcaacacgac cgtgctttcg ttgatcatct tgcaacgctt gagcacgtcg
     2581 agcccgctgt gctcgggcat cttgtagtcg gtgaccatca cgtcgaacac ttcggattcg
     2641 aggcgcgaaa acgcctcgga gccgtcggcc gcctcggcga cgtgccaacc gcggtcacgg
     2701 aacgccgtga cgatgccgtc ccgcagcgtc ggctcgtcct cgacgacgag gattttcttc
     2761 atcggccttc agacctccgc tcgatcgagg accatcggca ggcgcacggt gaacagcgcg
     2821 cccccctcgg gtgcatcggc gacgtcgatc agaccgtggt ggctgctcac gatcttcttg
     2881 gccatcgaca ggccgactcc ggacccatgt tttttcgtgg tgaagaacgg atagaacaca
     2941 cggtcgcgct cttcgctccc gatccccggc ccgctgtcgg cgatccgcac gacggcgaag
     3001 tgctcgaacc ggttccacgg gtcgttgccg cgatcgtccc ccatcgggta cgggatcgac
     3061 gtcgcgctcg gggccggccg catctcggtc tcgacgcgga tcacgccccg cccgtcgatc
     3121 gcctccatcg cattgatcag caggttctcg aacacctggc gcagctgtct cgggtccatc
     3181 aggaaccgcg gcatgtcggt cgcgaagtcg cagcgcacgt cgatcccggg cttgcccgcc
     3241 cgctgccgcg ccacgtgcag cgcctcgtcg agcaaggcgt tgagctcggc gtgcgccgtg
     3301 cgcagctcga gcggccggac gaactcgagg ctcgacgtga tcgtgccgtt cagccggcgc
     3361 acctcgccgg tgatcttcgt cagcaggtcg aagctcttct cgtcgccgcc gttctgcgac
     3421 gccaaccggc gctgcagcag cttgcatgtg acctcgatcg cggcgagggg gttgcgtatc
     3481 tcgtgggcca gactggctgc catctggccc agcgcggcca gccgatcctt cagccggtct
     3541 tgctcttcct tgtgctcgat ctgcgtcagg tccttgaaga acatcgccgt cccgaccgga
     3601 tcgcgttcgc ccggctcgcc cgggatgatc gacagcgtga agccgatcgt cttgctgcgg
     3661 ggatcgcccg gccgcaagtc aagctcggcg cggttcggca ggctcgtcat gtcgaacgac
     3721 tcaccgagca cccgggtgac gagcggaaac tcgcggagcg cgtcggcgac gggtgtggcg
     3781 accggcggtg cggcgtcgag ctcgaggatc tgtagcgcaa tccggttgac catcaacagc
     3841 tgcccgtccc gatcgacggc gaggatgccg caacgcatgc cgtcgacgat gccgcgcagg
     3901 agaccgagcg acggtccgac ggatgttgcc gcgtcgtcca ccgcgcgatc ggcaaacgca
     3961 acctctccat ggcagactgg tttggggttc gacgccggcc tcaacctgac ctcccgagtc
     4021 gcctggcaaa tctaggttcc ggcaagacgc ctggcaagga aacccaggct tttttcgtaa
     4081 ttaccgggcg tgatcgcacg atgagccaca aagtagtggc gtgccgcgct cagttggcgg
     4141 tcagcgtccc gccggcgaag acgatccctc cgatcgactg cggtggatcg gccgagttga
     4201 gcagcccggc ccgttcgaac cggacctgcg aggcaccggc ttgcgtgacg cggaagagca
     4261 gcttgacgag gcccgtgctg atcccggcgt tcgccccgcc gctcggcgtc gtccgcgcga
     4321 cgccgaccac gagcctgccg gcctgctggc tgttgatctg gtaggacacc tgctggttgt
     4381 tcgactcgag caggctgccc ggggaccagt tgacgaactc ggtcatcgtc ggatcgtagg
     4441 tcacgtcgaa cgacgcgccg aagacgtcgg tcacgcccgt caccgtcacg ttcaacgtga
     4501 cgatgttcgt gttcgacgag ccgtggatcg acaccgtgtt cgcccccggg tccgggttgt
     4561 ccggcgtgaa gttgccggcg aggatgcccg accctccgcc gggtccgccc gacagaccgg
     4621 aggtctcgcc tcctccgccg cacgccacga gcgaggtcac gagcaacagt ggcaacaggc
     4681 gggtggggtc gggacaacgg gttcgttcgt tgtgctggtg gatcatgatg gcttccgtcc
     4741 ggtagtgtgg tttgtcgtgg atctacaggg catagactag gtccggcggc gtcagttgca
     4801 agcggcgtcg gaccaggagg cgccggacca gcagcggccg aagcgggccg ccagcatggc
     4861 caggtcggat ccgtcgacca tgccgtcgcc gtcgagatcg gcatcggggt tgaagtacgg
     4921 ctgtccctcg cggctcgcgt gcgcgcgagc gagccaggtc aagtcctttc cgtcgacccg
     4981 ctcgaacgtg tcgccgtcgc tctggttgat gtcggcccgg atctcgtcga gctcgacctg
     5041 aatcgtctcg cgaccggttc cgaagaccgt atccggattg acgacttcga aatcgacgtt
     5101 gaatccgccg atctgcatcg cccgcagacc gcgcgccgcg ggttcgagcg tgacgtacgc
     5161 ccgcagctcc gtgcaggatt gcacggcgag gtcttcgagg acgatcagct cgttgccgtc
     5221 gatgtcgcga ggcacgccgt cgatgctcca ggccaacgcg gcgtccgggt cgaagttcgc
     5281 gccgaagacg tcgacggtca gccgggtctc accctggacg acgctcgtcg gcgtcatccg
     5341 atcgatcaac gggcgcgccc aacccgcgac ctcgttcgag aattgggagg aaatccctcc
     5401 gttggtgtcg tacgccttga tcgaaacgta gtaggtcgtg cagtccaaca gtccggtcaa
     5461 ggtgccgctc gtggcattct tcatgtcgac aacgcccgtg tactgaccgg atgcggtgcc
     5521 gaagtaggcc cggtagccgg tggcgccggg caccgcgtcc catgcgaggt ggatcgatcc
     5581 ggccgacgcc gcgcccggta gcgcggcaag gagaccggcg agcgcaagac ggcggaggag
     5641 ctgactccac atgtacgtcc ccacagatcc ccccgcggcg tgcatcgcaa tcgggatgcc
     5701 aggttttctg gagggccaca ccgatcggcc cgacaggcgc gaaaagggct cattcctgct
     5761 gtgggtcggc gcggccccgc caaggaccga cagtctacta cgcctggccg gaactcgaaa
     5821 acgaaagcgg gatgtccacg gccgcaatct tgctctcgga acggactgcg gtggagccga
     5881 tcggccgttc ggccttgcgg gacggcgccc gcggggttcg acggcttccg agccggggga
     5941 tttgtgtgtg cggatccaca cgacctgtgg ggagccaccc acgccgcggt ccggccggcg
     6001 gcgaacggtc gagtcggtgg ttgtttccgt tccgaagctg gtgttacgct gtcgtaatcc
     6061 ggtcggtagg ggtcgcgaag tcggggttcg tgaaccgatc tgttttcggg gtcagttcgc
     6121 tccgagcgtg agttttccgg ccgatggcac gaaatcggca cgagcggggt tcttcgagtg
     6181 cgccggaacg gccctcaggt gggagcgagt gtgggttgca tcggtcgatc gcgtttcgtg
     6241 cgcaccgcgt ggggcgccct cggtctggcc gtgcttgccg tcgcttgcgg cgtggcgcat
     6301 gcgggcgaag tgaacctgtc ctgggatccg tcgtccggcg cgtcgggcta ccgtgttttc
     6361 agcgggtcga gctccgggtc ctacggatcc ccgcaggacg tgggaagcgc gacgaccgtg
     6421 ccgatcacga cggtgcccga ctgcaccacg acctacttcg cggtcacggc ctacaacggt
     6481 gcgggtgaat ccggcttctc ccaggaagtg tcgagctggc cgcggccggt gctcagctcg
     6541 atcaacgatc cggacgccca gcgaggcgag tcggtcaccc tcgtgatcgg cggcgccaac
     6601 ttccaacccg gctcgtcggt cgcgatcccg gccacgggag tggtgctgaa ctcggccacg
     6661 atcgattcgt gcaatcagct cacactgggc gtcagcgtcc aggccaccgc gccgttcgga
     6721 ccggtcgaga tcctcgtgac caacggcagt ggggtcgagg ggcgcacgag cgcgctgttc
     6781 agcgtcgtcg gcaacccgct tccgggcgtc gagaacctgc gccgaaccga tacgacgaac
     6841 tgaaccgtcc ccctcccgcc ctcccccgtt gccgatccgc gatcgaccgc cgtaaccttg
     6901 cgattcgact cccgaggagc gttcccccgt gctcgaccga ctccgtcgtt ctggcctcgg
     6961 cgacgcggca cggcgttggc tgccgccgcc gctccgggcg gtgcttcacc ggctgagagg
     7021 cgcgctcgaa gcctcctccg acccgtttcg gacgtgcgag atcgcgctcg gccggaacct
     7081 gcgcgaggag tccgctcgat cggtcgaccg atggcgaacg acgggcgggg tcgacgactg
     7141 gcgccgcctg cgtgattcct tcctcaccga gctcggcggc tcgctcggtc cgtggcgacc
     7201 cgacgagcgg ccgctgcact cgcagacgac cggacgagtc gaaggggacg ggttccgggt
     7261 cgactgcctg gtgttcaccg ccagaccgga cgtccggatc accgcgaacc tctaccggcc
     7321 ggccgagtgc gactccccgc ggcccggcat cgtcttctgc cacggtcacc acgcggggaa
     7381 gtgtcagccc gagctgtggg aagcgggcgt cgagtgggcg cgcgccggtt gtgtcgtgct
     7441 gatccccgat ctggccggcc acggcgaacg gcgcgagcac ccgttcgcga gcccggccga
     7501 ccatcccggc ccgttccgcc ccgatcgcca ggactactgg ttccgctact cgctgggatt
     7561 gcagctggcg gcggtcggac acagcctgct cggttggatg gtccacgaca ccctgcgatc
     7621 cgtcgatctt ctggtcgcgc gaaacgacgt cgacccggca cgcatcgcgg tcgtcggatc
     7681 cgtcgcgggc ggcggggacg tcgcggccgt cgcggccgcg ctcgacccgc ggatcgacgt
     7741 cgtcgtgccg ttcaacttcg gcggcgcgat ggacgacccc ggcgccggcg gatgggacgc
     7801 gtcccgctcg ccacgccggc gagcgagcca cggcctggcg ccgtggatga tcctcgcgtc
     7861 ggtcgcgccg cgtcgcctgg tctacgccca cgagttcggc tgggaggccg cgaccgatcc
     7921 cgtctgggag cgactgcgcc ggacctggcg gatcttcgac gcggagtcgc gtctggcggt
     7981 cgtccacggc cgcgggcgga tctccggccg accgccgcac cacacccact gcaccagcgt
     8041 cggccggatc catcgcgagc gattggcccc tacgctcgcg cgctggctcg gtgtggcgtg
     8101 cccgcaaccg ggcgctccgc cgatgccgca cgaccgcctc cactgtctgc gtgacggcga
     8161 cgtgaaggac ggtctcgttc ccatccatcg gaccgtcgag cgttcggtga tcgaccgact
     8221 gacggaatca cgggccgccc ggcaccgact cgcgcccgcg gacgggcgga tcgccgtcgc
     8281 cctggcactg tcggcgaggc tcgggccgct cgacgagccg accgcggccc gattcgcgcg
     8341 ggtgtcgcgc gtcggcgcgg cggcgcgcgg cgtcgaacgt cacgagctgc gaaggccagg
     8401 gggagccaga gtgcggttcg atctccgcct gccgcgcgac gcttcctcgg cgcccttccc
     8461 gctggtcgtc ggtttgggac aggcgccggc gcatcgtctc gcacggcggc ggcgcgcatt
     8521 gatcgagcga ctctgcggtc gcggcatcgc cgtcgcgctc gcccagaccc gcgggtcggg
     8581 gccgacgcgc tacgacgatg ccgggtacgg ccggacgagc cgggcgacgg tcgtttccgc
     8641 gtcggcatgg atgctcgatc ggatgtggat cgccgcccgg ttgtcggacg tagccgaggt
     8701 gatgcgagcg ttggccgagc gcccggagat cgacgcgcgc cgtgtcgccg tatggggcga
     8761 aggggacgca ccgccgaatg ccgacggatc ggcgacgtcg ggcccgatcg gcgtcgagcc
     8821 cgagcccgtg gtcgcgagcc ccgtcggagg aatcgtcgcg ctcgcttggg ccgtcgggca
     8881 cggctcggtg cgcgccgtct acgtccaccg gggattggtc agcctcgcgt cgatcctcga
     8941 gagcccacgg gtctgcttgc cgcacgaggc ggtcatcccg ggcttgctcg agtcgctcga
     9001 cctccccgac ctcgccgccg ccctcgcccc gctcccgatt cgtctggaag cgctggtcga
     9061 cggtcgcaac cggccggtcg acggcgatcg attggagcgg gcctacgcct gcgcgcgcca
     9121 gcgctatcgc gatctcggcg aggacgatcg gctcgtgctg cgtccgctcg acgcggccga
     9181 cgagcgcgtg gccgattggc tcggcgaggc gttggtgtga ccttcgctag accgcaccgc
     9241 cgtcggcgac cagccgttcg atgcgctcga ccgctcgacc gaggtctccg tagcaaagct
     9301 cgaagcacgg gcagcgtccg atcatcgcgg agagcagctc gaacccgctc cggccgtaga
     9361 ccccgtagtt gaacgcgttg tcgacggccc gcacgaacgc ctgcgcacgg gccagcggcg
     9421 ccatgctcgc gtcttccccg tcggcgtaga tcgggaagat cacccaggcg ggttcggccg
     9481 tctccgcgga acgtgcgacg ctgtcgcgcg gtggcacgac gtgcgccacg gtccctttcg
     9541 ccgtgccggc ccacgacggt ccgatcgtcg ctccgggcgc gaagcggcgg atcacctcga
     9601 tcgatcgtcc cttgagactg atcggccggg gaaccgcgcg gagccttccg tccgacggat
     9661 cgaccagcgc cacctcgtcc gacagcaagc gccagccgcg ctcgacgaga ccggcgcaga
     9721 gcgtgctctt gccggacccg gcacgaccca ccaggatcgc cgcgcgtccg ttgcgggcga
     9781 cgacggccga gtgcagcatc aggtactggt gcacacgctg gaagacgctg acgttcatcg
     9841 cccattcgag catcggcacg acgagccggg gggacaacca gtcgtactgt atctcgccgt
     9901 cgacccggag cgtgacggcg acgggccaga agcgcttgcg gcgcacgatg aactccgcgt
     9961 cgacgaccgt cccgttgtcg caaagcggga agtccgcgta gagcaagcgg acgccgtcga
    10021 cggccgcggc gagcttcgtc cgcagccgca ccgcgaacgg gccgacctcc cacgcgagcc
    10081 cgcgaccggc ggcgcgccct cgcgcgtcgg cgcgagcgag ttcgccaacc gtcatccgac
    10141 cggctccgcc agcccgactc gatcgaggtg ctcgagcagc tcgcggactt gccgtgcgag
    10201 tccttcatcg ggcccgaccc cgagccgtgg cgcgatcgtc cgcgcgatcc ggctcgcggt
    10261 gcacggagac cgttcgagaa cgcggagcac ctcggcggtc acgctgttga cgagatgggt
    10321 gtcgccggag tcgcagtgat agacgatcga ctgctccgga tcccacgagc gccagacgag
    10381 accgtcccgc cccacgaggg cccatcgagg ctcgggctgc gaatgagaat cacccgccgt
    10441 catgaacggg gctcgagctc caccttccga ccgggcgcga acgccggact acatccggag
    10501 gaagtctcgg gtgtccgtct ggccggtcgt gtccacttcg tgggtcggct ggccggatcc
    10561 ggtacccagg gggcagtcga tgaactcttg cccggcggtg ctgctcggat cggcattcgg
    10621 gtcgtagctc gggttcctgc agcgcccggc gttcgcaccg tagcgatacg ccgtcagcgt
    10681 gtaatcggtc gatgcccaag ccggcgcggc gtgcagcgtg aggatcgtcg gcacgaggag
    10741 cgctcctacc ttcaacgccc gcctgcggtc gaggcgcgct tcggtcggag ccgtccccgc
    10801 tgtcgtcgtc ccgttgtctt cgttttccat gaccgcaacc cctacacgac ttcgtgctcg
    10861 acacgaatgt cgcgcacccg ttgggcccaa tataccgacg tcgcttcgaa ttgtacaact
    10921 cttacttctc cgtgtctcgg gccggtgacg aaaaatacga aatgtaacaa atataatttt
    10981 catggttcga tgccgggggc gtcgtgcaat cgggaatccc gggcgcgtat actcccgtcc
    11041 cccgcgtcgg cccgggtgcg gggcccactc gacgagaggt gaccgagcct tggcggaacg
    11101 ggagcatcgg accgacgagc gcggcgcggg actcggcgtg gacgatctga gagcgcactt
    11161 cctcgagaag tacggcccgc ccgaggcgac cggttgggga ccgcgtgcgc gattcgcgtt
    11221 cgactacttc acgccggccg accactacga agcctatgtc ggccggcgcg tcgacgagcg
    11281 gactcgctgg ctcgacgtcg gctgcggccg tgcgctcttc tcgagcgata cgatcgcgag
    11341 gcggctgtcg gatcgttgcg ggctgctggt gggcctcgac cccgacgaca acctggacga
    11401 gaacccgtac gtccacgaga aggtcaaggc gtcgctctac gactatcgaa cggagcgggc
    11461 gttcgatctg gttacgttca acatggtggt ggagcacatc accgatccgg gcgaagtcgt
    11521 tcgcgtactg tcgaaattga cggctccggg cggcgaggtg gtgatcttga ccgtgttcaa
    11581 gtggtcgccg attccgatcg cgacgaggct gatccccttt cggttccatc acgggttcaa
    11641 gcacatgc
//
LOCUS       DASPGN010000175         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1667661, whole genome shotgun sequence.
ACCESSION   DASPGN010000175 DASPGN010000000
VERSION     DASPGN010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1667661"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /gene="amrS"
                     /locus_tag="VD788_05050"
     CDS             complement(<1..501)
                     /gene="amrS"
                     /locus_tag="VD788_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXV75667.1"
                     /translation="MPADADYIVPTRFWHPLDDGRVQCDLCPRFCKLREGQRGLCFVR
                     ACQDGRVVLTSYGRSSGFCIDPIEKKPLNHFLPGTPVLSFGTAGCNLACRFCQNWDIS
                     KSREIDTLADRATPATIARAAAALDCRSVAFTYNDPVIFLEYAADAAIACREQGLRTV
                     AVTAGYV"
     gene            complement(545..1774)
                     /locus_tag="VD788_05055"
     CDS             complement(545..1774)
                     /locus_tag="VD788_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75668.1"
                     /translation="MLVDLLRDPAFWQYASIPFVAAFIGWITNWVAIQLTFRPLAYVG
                     WRPPFGWQGIIPAKASKMARIFVDRTMFRLGTLREVYQSMDPEKMAQHVTAVMDPRIE
                     EYTNELMFYEDPRLWQMLPDRVKRGIYARVREELPRLVRALIRDAGEQIDELIDLKEM
                     LVQRLEGDRGLLNRLFLQSGKVEFAFIIRSGLYFGFLFGLIQLAVWWYYKGWWVLPVF
                     GVIVGYATNWFALNIIFRPLHPIRVGPWRVQGLFLKRQREVAAVWCEIVTTEILTVQQ
                     MIYAMLYGSKSANTHALIRRHIGPVVDAAVESYRPLSDLAVGERTLDGLRSSVAAKAI
                     AVSTDPFDHWQFNRERARVIDRLLRERMESMPPDQFQELLRPCFQEDEFKLILTGAVL
                     GFVAGWAQLVLVFGGAG"
     gene            complement(1786..>3322)
                     /locus_tag="VD788_05060"
     CDS             complement(1786..>3322)
                     /locus_tag="VD788_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HXV75669.1"
                     /translation="LAAYLRTLPSWRRRQPRMLLLRCFAAVAGLPVSRRIKREPFPRQ
                     CRLRLEALGPTYIKLGQVLSLREDLLPRPLTDELKLLLDRLPAVPYAQFVERVREHLG
                     RPVEEVFAHIRSTPLGSGSIGQTHRATLLDGRQVVLKIVKPGVRETLRRDTYLLRGLG
                     AVLQLFLPRFQPRRLIDEFCSYTLREVDLEREADNAEMFAANFASQPQIVFPATHREH
                     SNRDLLCMDYLDGIKPGDPKTGLLREVDRERLIDLGSEAIVSMLYRDGFFHADLHPAN
                     LIILPGPRVGFVDLGMVGRLDTDLKRTLLYYYYCLVAGDAENAAHYLAAMAVAGPRAD
                     VRGFKRDVEEICRHWSHHSTYASYPLGRLILQSVGRGAQHRVYFPVEMVLMAKAIVTF
                     EAVGAMLLPDFDIAAVSRRHVLQVTLQRFAPLRLAQESLTALPELVDALAKTPRLVTE
                     GLQLVEQATRRPSENPFAGLRATLFGGACLVAGAILAGFGGPWPLWAALLLIGILLPL
                     RRK"
BASE COUNT          603 a         1094 c         1140 g          485 t
ORIGIN      
        1 cacgtagccg gccgtcacgg ccacggtgcg gaggccctgc tcccggcagg cgatcgcggc
       61 gtcggcggcg tactcgagga agatcacggg atcgttgtag gtgaacgcga cgctgcggca
      121 gtcgagcgcg gcggcggcgc gcgcgatcgt cgcgggagtg gcgcggtcgg cgagcgtgtc
      181 gatctcgcgt gacttgctga tgtcccagtt ctggcagaag cgacaggcga ggttgcaacc
      241 ggccgtgccg aacgacagca ccggcgtccc ggggagaaag tggttgagcg gcttcttctc
      301 gatcgggtcg atgcagaagc cgctcgagcg tccgtagctg gtcaggacga cgcggccgtc
      361 ctgacaggcg cgcacgaagc acagtccgcg ctggccttcg cgaagcttgc agaaccgcgg
      421 gcacaggtcg cactgcaccc gcccgtcgtc gagcgggtgc cagaatcgcg tcgggacgat
      481 gtagtcggcg tctgctggca tcggggcctc gcgccggcga actccgctcc cattatagac
      541 gggctcaacc ggcaccgccg aagacgagca ccagctgcgc ccagccggcg acgaacccga
      601 gcaccgcgcc ggtcaggatc agcttgaact cgtcctcctg gaagcacggg cgcagcagct
      661 cctggaactg gtccggcggc atgctctcca tgcgctcccg cagcaggcga tcgatcacgc
      721 gggcgcgctc ccgattgaac tgccagtgat cgaacgggtc ggtcgagacc gcgatcgcct
      781 tggctgcgac cgatgaacgg aggccgtcga gcgtgcgctc gccgacggcc agatcggaca
      841 gcggccggta gctctcgacc gccgcgtcga ccaccgggcc gatgtgacga cggatcagcg
      901 cgtgcgtgtt ggccgacttc gacccgtaca gcatggcgta gatcatctgt tgcaccgtca
      961 ggatctcggt cgtgacgatc tcgcaccaca cggcggcgac ctcgcgctgt cgtttgagga
     1021 acagtccctg cacccgccac gggccgaccc ggatcgggtg caacgggcga aagatgatgt
     1081 tcagcgcgaa ccagttggtc gcgtagccga cgatcacgcc gaagaccggc agcacccacc
     1141 atcccttgta gtaccaccag acggccagct ggatcagacc gaacaggaag ccgaagtaga
     1201 gcccagagcg gatgatgaac gcgaactcga cctttcccga ctgcaggaac agccggttga
     1261 gcagcccgcg atcaccttcg aggcgttgga cgagcatctc cttgaggtcg atcaactcgt
     1321 cgatctgctc gccggcgtcg cggatcaggg cccgcaccag acgcggcagt tcctcgcgga
     1381 cgcgcgcgta gatcccacgt ttgacgcgat cgggcagcat ctgccatagt cgcggatcct
     1441 cgtagaacat cagctcgttc gtgtactcct cgatccgcgg atccatcacc gcggtgacgt
     1501 gctgcgccat cttctccggg tccatcgact ggtagacctc gcgcagcgtg ccgagacgga
     1561 acatcgtccg gtcgacgaag atgcgcgcca tcttgctcgc cttggccggg atgatcccct
     1621 gccagccgaa cggggggcgc cagccgacgt aagccagcgg gcgaaaggtg agctggatcg
     1681 ccacccagtt ggtgatccag ccgatgaagg cggcgacgaa cggaatgctc gcgtactgcc
     1741 agaacgccgg gtctcgcagc aggtcgacga gcacggtcgg ccagtctact tgcgtcgcaa
     1801 cggcaacagg atgccgatca acagcagcgc ggcccacagc ggccaggggc cgccgaatcc
     1861 cgcgaggatc gcacccgcga ccaggcaggc accgccgaac aacgtcgcgc gcagtccggc
     1921 gaacgggttc tccgacggtc gccgcgtcgc ctgctcgacc agctgcagcc cctcggtgac
     1981 gagccgcggc gtcttggcca gcgcgtcgac cagttcgggc aaggcggtca ggctctcctg
     2041 ggccagacgg agcggagcaa accgttgcaa ggtgacctgc aacacgtgcc ggcgcgagac
     2101 cgcggcgatg tcgaagtccg gaagcagcat cgcgccgacc gcctcgaagg tgacgatcgc
     2161 cttggccatc aacaccatct cgaccgggaa atagacgcgg tgctgtgcgc cgcggccgac
     2221 cgattgcagg atcagccggc cgagcgggta ggacgcgtag gtcgagtggt gcgaccagtg
     2281 ccggcagatc tcctccacgt cgcgcttgaa cccacgcacg tccgcccgcg gaccggcgac
     2341 ggccatcgcc gccaggtagt gcgcggcgtt ctccgcgtcg cccgccacca ggcagtagta
     2401 gtaatagagc aacgtgcgct tgagatcggt gtcaagtcgg ccgaccatgc cgaggtcgac
     2461 gaagccgacg cgggggcccg gcaggatgat caggttcgcc gggtgcaggt cggcgtggaa
     2521 gaagccgtcg cgatagagca tcgagacgat cgcctcggat ccgagatcga tcagccgctc
     2581 gcgatcgacc tcgcgcaaca gaccggtctt gggatcgccg ggcttgatcc cgtcgaggta
     2641 gtccatgcac agcagatcac ggttgctgtg ctcccgatgg gtcgccggga agacgatctg
     2701 aggctggctg gcgaaattgg cggcgaacat ctcggcgttg tcggcttcgc gctcgagatc
     2761 gacctcgcga agcgtgtagc tgcagaactc gtcgatcaac cgccgcggct gaaagcgcgg
     2821 aaggaacagc tggagcaccg cgcccagacc acgcagcaag taggtgtcgc ggcgcaacgt
     2881 ctcgcgcacg ccgggcttga cgatcttcaa caccacctgg cggccgtcga gcagcgtcgc
     2941 gcggtgcgtc tgcccgatcg agcccgaacc gagcggcgtc gaacggatgt gggcgaacac
     3001 ctcctccacc ggccggccga ggtgttcgcg gacccgctcg acgaactgag cgtatggcac
     3061 ggccggcaag cggtcgagca gcagcttcag ctcgtcggtg agcgggcgcg gcagcaggtc
     3121 ttcgcggagg ctcagcacct gtccgagctt gatgtaggtc ggtccgagcg cctcgagccg
     3181 caggcggcat tgacgaggga agggctcgcg cttgatcctg cggctgacgg gcagaccggc
     3241 gaccgcggcg aaacaacgaa gtagcagcat gcggggctgg cgccgtcgcc aactcggcag
     3301 ggttcgcaga taggccgcca gg
//
LOCUS       DASPGN010000176         7815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_614234, whole genome shotgun sequence.
ACCESSION   DASPGN010000176 DASPGN010000000
VERSION     DASPGN010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614234"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /gene="neuC"
                     /locus_tag="VD788_05065"
     CDS             <1..1171
                     /gene="neuC"
                     /locus_tag="VD788_05065"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HXV75670.1"
                     /translation="MRQILFITGTRADFGKLKALMHAVDGHADYACRIFVTGMHTLKA
                     YGKTQIEVQKEGFRDLFVFHNQHRGEPMETALANTIEGLSRYVHEDRPDLLVVHGDRL
                     EALAGSIVGALNNIPVAHVEGGERSGTVDESIRHAVSKLSHLHLVANDEARGRLIQLG
                     EAPRSIFVIGSPDIDVMLSDKLPSLDAVKRHYEIGFERYALLLYHSVTTALRTLRRDT
                     EQLLAAIADSGYRYVVIHPNNDPGAEVILAAYEQLLVDRDRYRLFPSMKFESFLTLLK
                     SAQFIVGNSSAGVREAPVYGVPAVNVGSRQHARHRGPSILDVAHDKAAILAAIRRAAE
                     IEDRLPSDRHFGAGRSAERFIELLDSGELFDVSPQKTFNDVRVSEVGPDPGESRS"
     gene            complement(1234..1938)
                     /locus_tag="VD788_05070"
     CDS             complement(1234..1938)
                     /locus_tag="VD788_05070"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HXV75671.1"
                     /translation="MSTIALIPARAGSKGLPRKNLLPLAGKPLLLHSIDAARASGACD
                     QVVVSSDGREILELARRAGASALERPPELAGDDSPTSAAIEHAVAALALDEGDVIVLL
                     QPTSPLRRAADVAAAIEIHRRRGRPLLSVEARAHYPDKLLAIGSDGLTEELSPGAGTR
                     PRQLMRPCVKPNGAIYVFSVAEFRAAGDVPLAGACTYPMDCLASVDIDDADDLALAER
                     VLRQRQSEIDRQDLGP"
     gene            complement(1987..2715)
                     /locus_tag="VD788_05075"
     CDS             complement(1987..2715)
                     /locus_tag="VD788_05075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXV75672.1"
                     /translation="MIGAARWRPSRRSAVTALALLVPAPSIGVLVALSGPTDESGATT
                     TLAQAVWLLSKLWILLLPVAWHRFVDGRAPRVPRPAAAGMPAAVLSGALFAIVITSAY
                     ATLGRRWIDVGAAGERIAAAGLDSLPLYAGMALYWCTVNSLLEEYVWRWFVFTRCEAV
                     RPGLPAVLAAGLFFTLHHTIALAAFFDDARTVVLASIGIWVAGASWSWIYLRWRNIYA
                     AWLSHALADLAIFWIGYRIVFAAG"
     gene            complement(2712..3539)
                     /locus_tag="VD788_05080"
     CDS             complement(2712..3539)
                     /locus_tag="VD788_05080"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXV75673.1"
                     /translation="MRVRLQRRFGERPRSGRSSGRVRPHVGEGRIAAPGRLRDRLVRK
                     GFAPRHVAEIGVHHPDRCQLRGYIDDGTRCTLVEPEPDAVEAIRRRFGELAHVVLHAV
                     ALCDRHGRVEMIRRAASTYLAEVARPPAVVNDGYRPRERDRFSVPATTFDTIDDGTIE
                     LLSVDVEGSEWYVIERMVSRPAVISVETHGSRYRNPHADEIGDWMARNGYVVWYRDRT
                     DTVWVRPQRIRVGLGERVARIAADLRITARRLRKTLAAWGRARGGARRADRRRSTGR"
     gene            complement(3446..3964)
                     /locus_tag="VD788_05085"
     CDS             complement(3446..3964)
                     /locus_tag="VD788_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HXV75674.1"
                     /translation="MNDRTRWLSVPLAIVCLGPAGCGGSVVDDGMPGGGGPTVVRMVT
                     SRGVYDTGQRMVIQVVIQNGQNVGSVPFHLRYNPQVVDFIEPPTEGPFLGADGAMTVF
                     LAADPSGGEIVVGLSRFDPVGMSGSGLLAEFAFDAIAAGDCGFGFSAASVKDPEAADL
                     PAAFDPTSVRVE"
     gene            4035..4760
                     /locus_tag="VD788_05090"
     CDS             4035..4760
                     /locus_tag="VD788_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75675.1"
                     /translation="MQRHAVSSSHTGCEMPIEHYGSAGAAMIYVPTSGGGVDEFAGYG
                     LPQACRPWLEEGSLQVFAIDGWARNTLWNEALSGAERMRAYAVYERYVADELLPWVAE
                     RTGGPPPLVVGCSFGAYVAANLLFKRPASVAGACGLGGVYGLWHRLEGHHDETVYFHT
                     PLEYLPRLDDPAALAAIRRTSGFAIFAAADDEWLSSSLSMADIMRRKSLPHTLDLWPS
                     PADHHEQWWRRQLAVFLVRRFGG"
     gene            complement(4784..6283)
                     /locus_tag="VD788_05095"
     CDS             complement(4784..6283)
                     /locus_tag="VD788_05095"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HXV75676.1"
                     /translation="MNRPETVSNQRFSSRWALLLSVLGIAVGTGNIWRFPRIAAANGS
                     DVGAGGFLIAWVVFLLAWSIPLIIAEYALGQTGRMGTIGSFARLTRGRFAWMGAFVGF
                     VATAIMFYYSVVTGWCVYFTLATVGRPLPASPDEAVAIWDGFQRSGLPLAFHALALLF
                     GALACWQGVRSIERANKILVPALFAIVAIAALRALTLPGAGAGIAYLFRVDWTTLGDP
                     DVWLQALTQNAWDTGAGWGLILTYAAYMRREHGVVRNAVLTGVGNNTVSLVAATMIFG
                     FVFAVLGQRMETVEILEVMRNSGPASTGLTFIWMPQLFAAMPFGRPLAVLFFVALSFA
                     ALSSLISMIELATRNLVDLGVERHRAVALVAVGGFVLGLPSAINLRFFANQDNVWGVA
                     LLVSGAFVALGVVIHGARRLRGEVTTGDDWPLPAGWESVMRVLIPLEALVLVVWWLSF
                     AFGGAWYDPLAEYSIMTIGLQWGIVLAAFLALNGWLARRTGAENRRSGG"
     gene            complement(6338..7675)
                     /locus_tag="VD788_05100"
     CDS             complement(6338..7675)
                     /locus_tag="VD788_05100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV75677.1"
                     /translation="MRVAYVTAGGAGMFCGSCMRDNTLAAALLRLGVDVTLIPTFTPI
                     RTDEPDVSLERVFLGGLNLYLEQWSPWLGRLPRAARRLLDHPRLLRAISSWSLARRGA
                     ADGAIAVSLLRGEHGKQAALTDDLVEFVADSLDPDLVCLTNLLIAGFVPALKRRAAVP
                     VVVTLQGDDLFLDSLAAGDREAVLREMRRLAAWIDGFLVFSRDYRDRMAALLHVPDER
                     FQLVPIGLADPTSFARYPRANDPHRPTVGYLARICPEKGFELLVRAFVLLRRQTGMQT
                     ARLDFGGWLGRAERPFLAAQWRRLSDAGAAGAVRRHALPDRDSKARFLSGLDVFCVPA
                     VYREPKAIFVLEALAAGVPVVAPAHGCFPELLAATGGGRLVRPNDPVDLARALAELLG
                     DPERRARLGREGQRAVAGRFDDEQMARRTLDVWRGLVDRSERAETGAPSSRRG"
BASE COUNT         1276 a         2814 c         2684 g         1041 t
ORIGIN      
        1 gatgaggcag atcctgttca tcaccggcac gcgcgccgac ttcggcaagc tgaaggcgct
       61 gatgcacgcg gtcgacggcc acgcggacta cgcctgccgg atcttcgtca ccgggatgca
      121 cacgctcaag gcctacggca agacgcagat cgaggtgcag aaggaggggt tccgcgacct
      181 gttcgtcttc cacaaccagc accgtggcga gccgatggaa accgcgctgg ccaacacgat
      241 cgagggtctg tcgcgctacg tgcacgagga ccgacccgat ctgttggtcg tgcacggcga
      301 ccggctcgag gcgctggccg gctcgatcgt cggcgcgttg aacaacatcc cggtggccca
      361 cgtcgaaggg ggagagcgtt cgggcaccgt cgacgagtcg attcgtcacg ccgtcagcaa
      421 gctgtcgcac ctgcacctgg tggccaacga cgaggcgcgc ggccggttga ttcagctggg
      481 cgaggcgccg cggtcgatct tcgtcatcgg ctcgccggac atcgacgtga tgctgtccga
      541 caagctgccg agcctggacg cggtcaagcg gcactacgag atcggcttcg agcgctacgc
      601 gctgctgctc taccactccg tcaccaccgc gctccgcacg ttgcgacgcg acaccgagca
      661 gctcctcgca gcgatcgccg acagcgggta tcgctacgtc gtgatccacc ccaacaacga
      721 ccccggggcc gaggtgatcc tggcggcgta cgagcagctg ctggtcgacc gcgatcggta
      781 tcggctgttt ccatcgatga agttcgagag ctttctgacg ctgctcaagt cggcgcagtt
      841 catcgtcggc aattcgagcg cgggggtgcg cgaggcgccg gtctacggcg tgccggcggt
      901 caacgtcggc tcgcgccagc acgcccgcca ccgcggcccg tcgatcctcg acgtcgcgca
      961 cgacaaggcg gcgattctcg ccgcgatccg ccgggcggcc gagatcgaag accgtctgcc
     1021 gagcgatcgg cacttcggcg cggggcgcag cgccgaacgg ttcatcgagc tgctcgactc
     1081 gggcgagctg ttcgacgtca gcccgcagaa gacgttcaac gacgttcgcg tctccgaggt
     1141 cgggcccgat ccgggagagt cgcggtcgtg aggcgctaac cgagtgttgc ccggcggttc
     1201 ggcgtcgaga tgttcgccga cgacccgcgc gtgctacgga ccgagatcct gccggtcgat
     1261 ctccgactgg cgctgccgta gcacccgctc ggccagtgcg aggtcgtcgg cgtcgtcgat
     1321 gtcgacgctc gccaggcagt ccatcggata ggtgcaggcg ccggccagcg gcacgtcgcc
     1381 cgccgcgcgg aactcggcga ccgaaaacac gtagatcgcg ccgttgggct tgacgcacgg
     1441 gcgcattagc tgccgcggcc gggtgccggc gccggggctg agctcctcgg tcagtccgtc
     1501 gctgccgatc gccaacagct tgtcggggta gtgcgcgcga gcctcgacgc tcagcagcgg
     1561 ccgaccgcgc cggcgatgga tctcgatcgc ggcggcgacg tcggccgcgc ggcgcagcgg
     1621 ggaggtcggc tgcagcagca cgatcacgtc gccttcgtcg agggcgagcg ctgcgaccgc
     1681 gtgctcgatc gccgccgatg tcggcgagtc gtcgccggcg agctcgggcg ggcgctcgag
     1741 cgcgctggcc cccgcgcgcc gcgccagctc gaggatctcc cgtccgtcgg acgagacgac
     1801 gacttggtcg cacgcgccgc tcgctcgtgc cgcgtcgatg ctgtgcagca gcaacggctt
     1861 gcccgccagc ggcagcaggt tcttccgcgg caggcccttg ctgccggcgc gcgccgggat
     1921 caacgcgatc gtcgacatga cgtcctctcg accggatgcc gacccagcat accagcgaat
     1981 cgacgctcag cccgcggcga acacgatgcg ataaccgatc cagaagatcg ccaggtcggc
     2041 cagcgcgtgg ctcaaccacg cggcgtagat gtttcgccag cgcaggtaga tccacgacca
     2101 gctggcgccg gcgacccaga tgccgatcga ggccagcacg accgtcctcg cgtcatcgaa
     2161 gaacgcggcc aacgcgatcg tatggtgcag ggtgaagaac agaccggcgg cgagcaccgc
     2221 cggcagtccg ggccggaccg cctcgcatcg cgtgaagacg aaccagcgcc agacgtactc
     2281 ctcgagcagc gagttgaccg tgcaccagta gagcgccatc cccgcgtaga gcgggagcga
     2341 gtcgagcccg gctgccgcga tccgctcgcc ggcggcgccg acgtcgatcc agcgtcggcc
     2401 gagcgtggcg taggccgacg tgatgacgat cgcgaacagc gcgccgctca ggacggcggc
     2461 cggcatgccc gccgccgccg gtcgcggcac ccgcggcgcc ctgccgtcga cgaaccggtg
     2521 ccacgccacc ggcagcagca ggatccacag cttgctcagc agccagaccg cctgtgccag
     2581 cgtcgtcgtc gccccgctct cgtcggtcgg accggacagc gcgaccagca cgccgatcga
     2641 aggcgccggc acgagcagcg cgagcgcggt caccgccgag cggcgggacg gccgccagcg
     2701 ggccgcgcca atcaccggcc ggtcgatcgg cggcggtccg ctcgccgcgc cccgccgcgc
     2761 gcgcggcccc acgccgcgag cgtcttgcgc agccgccgcg cggtgatgcg caggtcggcc
     2821 gcgatccgcg cgactcgctc gccgagcccg acgcggatcc gctgcggtcg gacccagacc
     2881 gtgtcggtcc ggtcacggta ccagacgacg tatccgttgc gcgccatcca gtcgccgatc
     2941 tcgtcggcgt gtggattgcg gtaacgggag ccatgggtct cgaccgagat caccgccggc
     3001 cggctgacca tccgctcgat cacgtaccac tcgctgccct cgacgtcgac gctcaacagc
     3061 tcgatcgtac cgtcgtcgat cgtgtcgaag gtcgtcgccg ggacgctgaa gcgatctcgc
     3121 tcgcgaggac ggtagccgtc gttgacgacc gccggcgggc gcgcgacctc ggcgaggtag
     3181 gtcgaggctg cgcgccggat catctcgacg cggccgtggc ggtcgcacag cgcgaccgcg
     3241 tgcaacacca cgtgcgcgag ctcgccgaac cgccgccgga tcgcctcgac cgcgtccggt
     3301 tcgggctcga ccagcgtgca gcgcgtgccg tcgtcgatgt agccccgcag ctgacagcgg
     3361 tcggggtgat ggacgccgat ctcggcgacg tgccgcggag cgaagccctt gcggacgagt
     3421 ctgtcacgca gccgaccggg cgccgctatt cgaccctcac cgacgtgggg tcgaacgcgg
     3481 ccggaagatc ggccgcttcg gggtctttca ccgaagcggc gctgaagccg aacccgcaat
     3541 ctcccgccgc gatcgcgtcg aaggcgaact cggccagcaa tcccgaaccg ctcatgccga
     3601 ccggatcgaa ccgcgacagg ccgaccacga tctcgccgcc gctcgggtcg gcggccagga
     3661 agacggtcat cgcgccgtcg gcgccgagaa acgggccttc cgtcggcggt tcgatgaaat
     3721 cgaccacctg tgggttgtag cgcaggtgaa acggcaccga gccgacgttc tggccgttct
     3781 ggatgacgac ctggatcacc atgcgctgtc cggtgtcgta gaccccgcgg gaggtgacca
     3841 tccgtacgac ggtcggaccg cctccgccgg gcatgccgtc gtcgacgacg ctgccgccgc
     3901 agccggcggg ccccaggcag acgatcgcca gcggcaccga cagccatctc gtgcggtcgt
     3961 tcacgctcgc tccagcccgc tccgggcgtt ttgtcgccga gagtatacga tgttcgcccc
     4021 tatactgcga cccgatgcag cgccacgccg tgtcctcctc ccacaccggt tgcgagatgc
     4081 cgatcgagca ctacggatcg gccggggccg ccatgattta cgtgccgacc tcgggcggcg
     4141 gcgtcgacga gttcgccggc tacggcctgc cgcaggcctg ccgcccctgg ctcgaagagg
     4201 gcagcctgca ggtcttcgcc atcgacggct gggcgcgcaa caccctgtgg aacgaggcgc
     4261 tgtcgggggc cgagcgaatg cgggcctatg cggtctacga gcggtacgtc gccgacgagc
     4321 tgctgccgtg ggtcgccgag cggaccggtg gtccgccgcc gctcgtcgtc ggctgcagct
     4381 tcggcgccta cgtggccgcc aacctgttgt tcaagcggcc ggcgagcgtt gccggcgcct
     4441 gcggtctcgg cggcgtctac ggtctgtggc accgactcga aggccaccac gacgagaccg
     4501 tttacttcca cacgccactc gagtacctgc cgcgtctgga cgacccggcg gccctggccg
     4561 cgatccgccg cacctcgggc ttcgccatct tcgccgccgc ggacgacgag tggctgtcga
     4621 gctcgctgtc gatggccgac atcatgcggc gcaagtcgct gccgcacacg ctcgacctgt
     4681 ggccgtcgcc cgccgatcat cacgagcagt ggtggcgccg gcagctggcg gtcttcctgg
     4741 tacgccggtt cggcggttga tccgggggtc ggtctcggcg acgtcaacca cctgagcggc
     4801 gattctcggc gcccgtgcgc cgggccagcc agccgttgag cgcgaggaac gcggcgagca
     4861 cgatccccca ctgcaggccg atcgtcatga tgctgtactc ggccagcggg tcgtaccacg
     4921 ccccgccgaa ggcgaacgac agccaccaca cgacgagcac cagcgcttcg agcgggatca
     4981 gcacgcgcat caccgactcc cagccggccg ggagcggcca atcgtcgccg gtcgtcacct
     5041 cgccgcgcag ccgccgcgcg ccgtgaatca cgacgccgag agcgacgaac gctccggata
     5101 cgagcagcgc gacgccccag acgttgtcct ggttggcgaa gaatcgcagg ttgatcgcgc
     5161 tcggtaggcc gagcacgaag ccgccgaccg cgaccaacgc caccgcgcga tgacgctcga
     5221 caccgagatc gacgagattg cgggtcgcga gctcgatcat cgagatcagc gacgacagcg
     5281 cggcaaacga cagcgcgacg aagaacagca cggccagcgg tcgaccgaac ggcatcgcgg
     5341 caaacagctg cggcatccag atgaaggtca gtccggtcga ggccggcccg ctgttgcgca
     5401 tcacctccag gatctcgacg gtctccatcc gctggccgag cacggcgaag acgaagccga
     5461 agatcatcgt cgcggcgacc aacgagacgg tgttgttccc gacgccggtc agcaccgcgt
     5521 tacgcacgac gccgtgctcg cgacgcatgt atgcggcgta ggtcaggatc agcccccaac
     5581 ccgcccccgt gtcccacgcg ttctgcgtca acgcctgcag ccagacgtcg ggatcgccga
     5641 gcgtcgtcca gtcgacccgg aacagatagg cgatgccggc cccggcgccg ggcagcgtca
     5701 gcgcgcgcaa cgccgcgatc gccacgatcg cgaacagcgc ggggacgagg atcttgttcg
     5761 cccgctcgat cgagcggact ccctgccagc aggcgagcgc gccgaacagc aacgccagcg
     5821 cgtggaacgc gagcgggagc cccgagcgct ggaagccgtc ccagatcgcg accgcctcgt
     5881 cgggggacgc cggcagcggc cgccccacgg tggccaaggt gaagtagacg caccaaccgg
     5941 tgacgacgct gtagtagaac atgatcgcgg tggcgacgaa gccgacgaac gcgcccatcc
     6001 aggcgaagcg cccgcgagtc aagcgggcga agctgccgat cgtgcccatc cggccggtct
     6061 gaccgagcgc gtactccgcg atgatcagcg ggatgctcca ggccagcagg aagacgaccc
     6121 acgcgatgag aaagccgccg gccccgacgt cactgccgtt ggccgccgcg atccgcggaa
     6181 aacgccagat gttgccggtg ccgaccgcga tgcccagcac gctcaacagc aaggcccaac
     6241 gcgaggagaa tcgctggttc gacaccgtct ctggtcggtt catctgcgcg aggacctcgt
     6301 ccgagccccc gtttgtctca cgatcgcgcc gggcgggtca accgcgtcgg ctgctcggcg
     6361 ccccggtttc ggctcgctcg ctccgatcga ccaacccgcg ccagacgtcg agcgttcggc
     6421 gcgccatctg ctcgtcgtcg aaacggccgg cgacggcccg ctggccctcg cggcccagcc
     6481 gcgcgcgccg ctcggggtcg ccgagcagct cggcgagagc gcgggccagg tcgaccgggt
     6541 cgttcggccg cacgagacgg cccccgcccg tcgccgccaa gagctcgggg aagcagccgt
     6601 gcgccggcgc gacgacgggc acccccgccg ccagcgcctc gagcacgaaa atcgccttcg
     6661 gctcgcgata gaccgccggg acgcagaaca cgtcgagccc tgagagaaac cgggccttgc
     6721 tgtcgcggtc ggggagcgcg tgccgacgga ccgcgcccgc ggcaccggcg tcggacagcc
     6781 gtcgccattg cgcggcgagg aacgggcgct ccgcccggcc gagccagccg ccgaagtcga
     6841 gacgcgccgt ctgcatcccg gtctgccgcc gcagcaggac gaaggcccgc accaacagct
     6901 cgaatccctt ctccgggcag atcctcgcca ggtagccgac cgtcggtcga tgggggtcgt
     6961 tcgcccgcgg gtagcgcgcg aacgacgtcg gatcggccaa gccgatcggc accagctgga
     7021 agcgctcgtc gggcacgtgc agcagcgcgg ccatccggtc gcgataatcg cgactgaaga
     7081 ccaggaaccc gtcgatccac gcggcgaggc gtcgcatctc gcgcagcacc gcttcgcggt
     7141 ctccggcggc caacgagtcg aggaacagat cgtcaccctg aagggtgacg acgaccggaa
     7201 ccgcggccct ccgcttcagc gcggggacaa agccggcgat caacaggttg gtcagacaga
     7261 cgagatcggg gtcgagcgaa tccgcgacga actcgacgag gtcgtcggtc agcgccgcct
     7321 gcttgccgtg ctcgccgcgc agcagcgaaa cggcgatcgc gccgtctgcc gcaccgcgtc
     7381 gcgcgagcga ccaggaggag atcgcgcgga gcaagcgggg gtgatcgagc agccgccgcg
     7441 ccgcgcgggg cagacgcccg agccacggtg accactgttc gagatacagg ttcagcccgc
     7501 cgagaaagac gcgttcgagg ctgacgtccg gctcatccgt gcggatcggg gtgaaggtcg
     7561 ggatcagtgt gacgtcgaca cccagacgca acagcgccgc cgcgagcgtg ttgtcccgca
     7621 tgcagctccc gcagaacatc cccgcgccgc cggccgtgac gtaggccacc ctcaccggcg
     7681 atcgcccgac cggtgcagcg cgagccacag cccggccgcg ccgcacagtg tgatcgtcgc
     7741 gccggccagc cgcgccgcgc cgtcgtcgaa cagcacggtg gcgttgacga tcatgaacag
     7801 gaagaacgcg tggaa
//
LOCUS       DASPGN010000177         5635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1433837, whole genome shotgun sequence.
ACCESSION   DASPGN010000177 DASPGN010000000
VERSION     DASPGN010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433837"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VD788_05105"
     CDS             <1..457
                     /locus_tag="VD788_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75678.1"
                     /translation="APAIAGETTVGRFVEELAKVKNLNATDPAIAADSLRAVGVRLDR
                     GLDLNKRLTESDVAEISRAAGLLVTTSNPDAFFDSGKVDRFFQAFQTELALGASTTAC
                     DPATEDCSNPGQGSGPGNGESGPPFDPFSKGRGNPRGKGKGDRTPTDPE"
     gene            613..3195
                     /locus_tag="VD788_05110"
     CDS             613..3195
                     /locus_tag="VD788_05110"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXV75679.1"
                     /translation="MDANRSPSLTAIFALFVLSGATSLVYEVLWLRQLILIFGSTQFA
                     TSTILSTFMGGLALGAFLVGRRLSGRGIRPLGIYGALEIGIGLYALLVPTLLGALSPI
                     YRALWQAGASESFVVLSLGKFVGIAAILLPPTVLMGASLPVLARQVADDPDRIGGKVG
                     LLYAVNTFGAVLGTFVAGFVAIPGIGMQRTLWVTAAVNVALGLAAIALVRRYPAVVVS
                     RGRSPVAGRAAPSGGMRLVLLAFALSGFGALVLEVAWTRVLALVMGSSVYAFALMLLA
                     FLIGLAAGGAVFSAVLRRLPALDPAVLLAALLGAAGVLAFATAFAFNALPRLFGEIYF
                     AFHPGPTGWFAVQLVFGLMIMFPATFALGGIFPAVLQLHARSLDHVTSSVGTVYAANT
                     AGTIVGAAAAGFLLLPTLGVLRTVVAVAILEIVLGMVVAIVMVRGSTRRRTVLAAPIG
                     IALVLMVLFAPNWDVRMMNSGVYMNLFDVSDEQGWASFERMVYENNRVVYAAEGITAT
                     VFVADQPEFDNRYLSVNGKIEASTVADLETQLMCAHLPLLLHDEPRDVLIVGLASGIT
                     VGAAAAHPVDSIRVVEIEKAMIPAARLFAESNNRVLDDGRVSLSINDARNELEFSSTT
                     YDVIISEPSNPWMTVAANLFTEEFFRMARTRVRPGGVFSQWIQTYYLPADDLRSIIAA
                     FRAAFPNVMLFETIDGIDLLLLGSDDPLELDLDALAGRMTELDVKMDLARVGMRHPID
                     VVSMFRLGTGEIDALTAGAPRNTDDNARVEFSAPKTLGVSTVVENLEMIRAHRADPLD
                     YLVPPPTDPIDRDRLRMGLAEASIRRGDLELAEEHARQVTDPPLKARAERLLEQARGG
                     ESNL"
     gene            3495..>5635
                     /locus_tag="VD788_05115"
     CDS             3495..>5635
                     /locus_tag="VD788_05115"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXV75680.1"
                     /translation="MVSRRFPPRGRVSSCLVAALWVCVLPAAAQTVYVDDDTCPAVGS
                     GTDPDPYCRIQDAICALNAGSGGTVMVRPGTYDESLRMFPGISVISTDGPAVTMINAD
                     GRPCITQDCLPSAENLSCSAVVWGQGATNDDRLEGFRITGGSGLFRDFGGGDPPDALA
                     GGGIFVFDSSPTITNNEIVDNALSHANATKEFWGGGIYLGGGSYAGPTQPVITYNLIQ
                     ENAADPDTGNSNGLGGGIYVGLYSAPVIDSNTIRSNKAGDTNKTNQRGGGGGLVVYSI
                     SPAVTPTISMNLIQDNVAADFGGGLALSQAFLGSVDFPSQGKVENNVFELNRSFSGGA
                     LNTTTTNGLITGNTFADNTADFGGAITIATSENLDDQATLINNLVAFNTSVLYGAGGV
                     GVYYSSPSLSNNLVFGNIPNDIDGALEEDDVIGVDGNLSADPLFVSREPGARDLRLTP
                     GSAAVDVGDNSQAPDRDFDNGPRVLDGDSDGVATIDIGAYELVLDTDGDGEPDYLDGD
                     DDGDGVADAADCAPADPGASIPPQAVGNTLIAVGGGPQIDWTWSPSGFANVYNFYRGT
                     QDAQSGWSYALECVVADTPWPNAEDPTTPAAGVLYYYLVAAASPCGDSVAGMASDGTE
                     IYAVPGCALGSADQDDDGTVDRLDNCVFDPNAQQIDTDRDGRGNVCDSDDDDDGLPDA
                     GDNCPLEPNANQADGDGDGDGDVCDNCPATP"
BASE COUNT          865 a         1975 c         1867 g          928 t
ORIGIN      
        1 tgctccggcg atcgccggtg agaccaccgt cggacggttc gtggaagagc tcgcgaaggt
       61 gaagaacctg aacgcgacgg atcctgccat cgcagccgat tcacttcggg cagtcggcgt
      121 ccgcttggac cgggggctgg atctcaacaa gcgtctgacg gaatccgacg tggccgagat
      181 ttctcgcgcc gccgggctgc tggtcacgac cagcaatcca gacgccttct tcgacagcgg
      241 gaaggtggat cggttcttcc aggcgttcca gaccgagttg gcgctgggcg ccagcacgac
      301 tgcgtgcgac ccggcaacgg aagactgctc caacccgggt cagggcagcg gccccggcaa
      361 cggcgagagc ggcccgccgt tcgacccgtt cagcaagggt cgtggcaatc cgcgcggtaa
      421 gggcaagggc gaccgcacac cgaccgatcc cgagtaagct cggttccggt cagagagaat
      481 gagaccgccc tcacccccca cggggtgagg gcggtttttt gttggtgccg gcgggcgttg
      541 cgaaatcttg tgtcgtagat tgtccgggtt cgccacggca atccctgacg acgccatttc
      601 cgaggccccc cgttggacgc caaccggtct ccttcgctga ccgccatctt cgcgctgttc
      661 gtcctgtccg gggcgacctc gctcgtctac gaggtgcttt ggctccgtca gctgattctg
      721 atcttcggct cgacgcagtt cgccaccagc acgatcctgt cgaccttcat gggtggcctc
      781 gccctcgggg cgttcctcgt cggccgccgt ctcagcggcc gcgggatacg gccgctcggc
      841 atctacggcg ctctcgagat cggcatcggc ctgtacgcgc tgctcgtccc gacgttgctc
      901 ggtgccctct caccgattta tcgcgccctg tggcaggccg gcgcgtccga gtcgttcgtc
      961 gtgctcagcc tgggcaagtt cgtcgggatc gcggcgatcc tgctgccccc gaccgtgctg
     1021 atgggggcga gcctgccggt gttggcccgt caggtggcgg acgatccgga ccggatcggt
     1081 ggcaaggtcg gtctgctcta cgccgtcaac acgttcggcg cggtgctcgg caccttcgtc
     1141 gccggcttcg tcgcgatccc gggtatcggc atgcagcgga cgctatgggt cacggccgcc
     1201 gtcaacgtcg cgctcggcct cgccgcgatc gcgctggtcc gacgctaccc ggcggtggtc
     1261 gtctctcgcg gccggtctcc ggtcgccggc cgtgcggctc cgtccggcgg gatgcgtctc
     1321 gtgttgctcg ccttcgcctt gtccggcttc ggggcgctcg tgctcgaagt cgcgtggacc
     1381 cgcgtcctgg cgctggtgat gggcagctcg gtctacgcct tcgcgctgat gctgctcgcc
     1441 ttcctgatcg gtctcgcggc cggcggcgcg gtcttttccg ccgtcctcag acgactcccg
     1501 gccctcgatc cggcggtgct gctcgccgcg ctgctcggcg ccgccggtgt gctggccttc
     1561 gccacggcgt tcgccttcaa cgcgttgccc cggctgttcg gcgagatcta cttcgcgttc
     1621 cacccgggtc ccaccgggtg gttcgcggtg cagctcgtct tcggcctgat gatcatgttc
     1681 ccggcgacct tcgcgctggg cggcatcttc ccggcggtgc tccagctcca cgcccgcagc
     1741 ctcgaccacg tcacctcgtc ggtcggcacc gtctacgccg cgaacaccgc gggcacgatc
     1801 gtcggtgccg cggcggccgg cttcctgctg ctgccgacgc tcggcgtact gcgcacggtg
     1861 gtcgccgtcg cgatcctcga gatcgtgctc ggcatggtcg tcgcgatcgt gatggttcgg
     1921 gggtcgacgc gtcggcgcac ggtgctcgcc gcgccgatcg ggatcgcgct cgtgctgatg
     1981 gtgctgttcg cgccgaactg ggacgtccgc atgatgaaca gcggggtcta catgaacctg
     2041 ttcgacgtgt cggacgagca gggctgggcc tcgttcgagc ggatggtcta cgagaacaac
     2101 cgcgtggtct acgcggccga agggatcacg gccaccgtgt tcgtcgccga tcaacccgag
     2161 ttcgacaacc gctatctgtc ggtgaacggg aagatcgagg cgtcgaccgt cgccgacctc
     2221 gagacgcagc tgatgtgcgc ccaccttccg ttgctgctgc acgacgaacc gcgggacgtg
     2281 ctgatcgtcg gcctcgccag cggcatcacg gtcggcgcgg cggcggcgca cccggtcgac
     2341 agcatccggg tggtcgaaat cgagaaggcg atgatcccgg ccgcccggct gttcgccgaa
     2401 tcgaacaacc gagtgctcga cgacgggcgc gtctcgctgt cgatcaacga cgcgcgcaac
     2461 gagctggagt tctcctcgac gacctacgac gtcatcatca gcgagccgtc gaacccgtgg
     2521 atgacggtcg ccgccaacct gttcaccgaa gagttcttcc gcatggcgcg cacgcgcgtc
     2581 cgtcccggcg gcgtgttcag tcaatggatt cagacctact acctgccggc cgacgatctc
     2641 cgctcgatca tcgccgcctt ccgcgcggcc ttccccaacg tgatgctgtt cgagacgatc
     2701 gacgggatcg acctgctgct gctcggctcg gacgacccgc tcgagctcga tctcgacgcg
     2761 ctcgccggca ggatgaccga gctcgacgtc aagatggacc tggcacgcgt cggtatgcgc
     2821 cacccgatcg acgtcgtcag catgttccgg ctgggcaccg gcgagatcga cgcgctgacc
     2881 gctggcgcgc cgcgcaacac ggacgacaat gcccgcgtcg agttctcggc gccgaagacg
     2941 ctcggcgtca gcacggtcgt ggagaacctg gagatgatcc gtgcccaccg ggccgacccg
     3001 ctcgactatc tcgtgccgcc gccgaccgat ccgatcgacc gggaccggtt gcgcatggga
     3061 ctggcggagg cgtcgatccg ccgcggtgat ctcgagctgg ccgaagagca cgcccgtcag
     3121 gtgaccgatc cgccgctgaa ggcgcgcgcc gagcgcctgc tggaacaggc acgtggcggc
     3181 gaatcgaatc tctgaccatt cggacggcga ccaaaagtaa cgattgccga gcgccgggcc
     3241 cgttcgtata tcaccaggtg cgtcgggaat gggggttcgc acctcgccgc cgacgtcgag
     3301 tcccgggtcg tcacctcgca caggaggacg tcatcgtcgc ggcagggccc agcggtgtcg
     3361 aagcggaccc gcggaccgcg gaaaccgacg ggaggccggt ctgccgggcc ttgcggcgcc
     3421 acgacgccga ccgtatacac ccttccgccg ggcccgctcc gctctctcgc ggttcgtgcc
     3481 cggccgagcg attgatggtt tctcgacgct ttccacccag gggccgggtc tcgtcgtgtc
     3541 tcgtcgcggc gctgtgggtc tgcgtcttgc ccgcggccgc ccaaacggtc tatgtcgacg
     3601 acgacacctg tccggcggtc ggctcgggca ccgatccgga tccgtactgc cggattcagg
     3661 acgcgatctg cgcgttgaac gccggcagcg gcgggaccgt gatggtccga cccggaacct
     3721 acgacgagtc gctgcgcatg ttccccggga tcagcgtcat ctcgaccgac ggcccggcgg
     3781 tgacgatgat caacgccgac ggacggccgt gcatcacgca ggactgcctg ccgagcgccg
     3841 agaacctcag ttgctcggcg gtcgtctggg gccagggtgc gacgaacgac gatcgtctcg
     3901 aggggttccg catcaccggg ggcagcggtc tgttccgaga cttcggcggc ggagacccgc
     3961 ctgacgcgct ggccggcggc ggcatcttcg ttttcgacag ctcgccgacg atcacgaaca
     4021 acgagatcgt cgacaacgcg ctgagtcacg ccaacgcgac caaggaattc tggggcggcg
     4081 ggatctacct cggtggtggt agctacgccg gtccgaccca gccggtgatc acctacaacc
     4141 tgatccagga gaacgccgcc gacccggata ccggcaactc gaacggcctc ggcggcggca
     4201 tctacgtcgg cctttacagc gccccggtga tcgacagcaa cacgatccgg agcaacaagg
     4261 ccggcgacac gaacaagacg aaccagcgcg gcggcggcgg cggcctcgtc gtctacagta
     4321 tttcgcccgc cgtcacgccg acgatctcga tgaacctgat ccaggacaac gtcgcggccg
     4381 acttcggcgg tgggctcgcc ctgtcacagg ccttcctcgg atcggtcgac ttcccgtcgc
     4441 aaggaaaggt cgagaacaac gtcttcgagc tcaaccgcag cttcagcggc ggcgcgctca
     4501 acacgacgac gaccaacggt ttgatcaccg gcaacacctt cgccgacaat accgccgatt
     4561 tcggcggggc gatcacgatc gcgaccagcg agaatctcga cgaccaggcg accctgatca
     4621 acaacctcgt cgcgttcaac acctcggtgc tgtacggtgc gggcggcgtc ggggtctact
     4681 actccagtcc gtcgctgtcg aacaacctgg tgttcggcaa catccccaac gacatcgacg
     4741 gggcgctcga ggaagacgac gtgatcggcg tcgacggcaa tctctccgcc gacccgctgt
     4801 tcgtcagccg ggagccgggc gcccgcgacc tgcgcctgac ccccgggagc gccgcggtcg
     4861 acgtcggcga caacagccag gcacccgacc gggatttcga caacggcccg cgtgtgctcg
     4921 acggcgactc cgacggcgtc gcgacgatcg acatcggcgc ctacgagctc gtgctcgaca
     4981 ccgacggcga cggcgagccg gactacctcg acggcgacga cgacggcgac ggcgtggccg
     5041 acgcggccga ttgcgcaccg gccgatcccg gcgcgtcgat tccgccgcaa gcggtcggca
     5101 acacgctgat cgccgtgggt ggcggcccgc agatcgactg gacgtggagc ccgagcgggt
     5161 tcgccaacgt ctacaacttc taccgcggaa cccaggatgc ccagtccggt tggagctacg
     5221 cgctcgaatg cgtggtggcc gacacaccct ggccgaacgc agaggacccg acgacgcccg
     5281 ccgccggcgt cctgtactac tacctggtcg ccgcggcgag cccgtgcggg gacagtgtcg
     5341 ccggcatggc ctccgacggc accgagatct acgccgtccc cggctgtgcg ctcggctccg
     5401 ccgaccagga cgacgacggc accgtcgatc gattggacaa ctgcgtgttc gacccgaacg
     5461 cgcagcagat cgacaccgac cgcgacggtc gtggcaacgt ctgcgactcg gacgacgacg
     5521 acgacggcct gccggacgcc ggagacaact gcccgctcga gcccaacgcg aaccaggccg
     5581 acggcgacgg agacggcgac ggcgacgtct gcgacaactg cccggcgacc ccgaa
//
LOCUS       DASPGN010000178         5784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1668392, whole genome shotgun sequence.
ACCESSION   DASPGN010000178 DASPGN010000000
VERSION     DASPGN010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668392"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VD788_05120"
     CDS             <1..336
                     /locus_tag="VD788_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXV75681.1"
                     /translation="HSTNCPNVIQLMYDPERRIDVDWSTGQEDGEALFDVKLMLDVEN
                     RQGLLAKIVSTVADEKINIKNVEADAFELADAKIQIVLSVSDRKQMDRVIRSIRRIKG
                     VRDVERVRR"
     gene            complement(351..548)
                     /gene="rpmB"
                     /locus_tag="VD788_05125"
     CDS             complement(351..548)
                     /gene="rpmB"
                     /locus_tag="VD788_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXV75682.1"
                     /translation="MARICGICGKKTVFGNNVSHAHNVTSRRWLPNLRRVRVRTQGGG
                     RKRVRICTRCLRSGKVEKAVS"
     gene            1003..2076
                     /gene="pilM"
                     /locus_tag="VD788_05130"
     CDS             1003..2076
                     /gene="pilM"
                     /locus_tag="VD788_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HXV75683.1"
                     /translation="MLFGRTRNLVGLDIGDSSVKVVELKELGKGKGYQLLSLGWEPLS
                     AEAIVDGQIMDSQLVAETVQRLCQRCRIKSGSQVSVALSGHHVIVKRISLPQMSEAEL
                     AESIHWEAEQYIPFDIDDVNLDYQILEGSSLSGEGGNMDVLLAAAKTDKINDYVSVVS
                     DAGLNAVTVDIASFAMQNAFEANYEFDPVHVIALVDIGSAVSSISVLHGGTSVYWRDI
                     NIGGNQYTDAIQKELNLSADQAEQLKRGEEIDGIPYERVLPILSSVSDDIGTEIQKTL
                     DFFKQISATDRPLDRLYLTGGTAQVVHMKESLGERLGTQVELLNPFRKIPPAGRDASP
                     ELVNEMMPTASVAVGLALRKFGD"
     gene            2081..2665
                     /locus_tag="VD788_05135"
     CDS             2081..2665
                     /locus_tag="VD788_05135"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXV75684.1"
                     /translation="MIKINLLAEKKQPKPERASTIKVEMGGGQNFLLVGILLVGVVIA
                     GFWWWSASSELEDWKRKHVEADAELQRLEAIRAKGEEYKKKKEELARKIDLITRLKKQ
                     QAVPVHILDQISKNLPDFLWLESMSVANNNIVINGKATSYNAVTNFYNNLGDSGYFGS
                     VTLGRVFEVKEGVAFGITCSFAGLKTEPAVLPQN"
     gene            2679..3254
                     /gene="pilO"
                     /locus_tag="VD788_05140"
     CDS             2679..3254
                     /gene="pilO"
                     /locus_tag="VD788_05140"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HXV75685.1"
                     /translation="MFERVPFYGQVLIFLAMAAGIVLVAYTVYPNLGAMQDEITQLEN
                     DYAVKEAKIREGQAIEQRLPEFEREIETLQRKLGDIQQILPTNQETGDLLAWIKNVGD
                     QSNLDLKSFSPGGLKPVDFYKEFPIEMQVVTRYHDLGIFLDKVSKYSRIINVDNLRIN
                     QSSERDKTISASFTATTFVYDENANPQGGVQ"
     gene            3251..3817
                     /locus_tag="VD788_05145"
     CDS             3251..3817
                     /locus_tag="VD788_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75686.1"
                     /translation="MNRRRLIWFATIVTLTLVVAVSSPGFIAQSVSAATEEQPTEPAG
                     ETGETGEEAVEQILRQQEALITGQGFSYDPEGRRDPFRSPFGAIGNTERKRPPGMAGM
                     LVAEIDLTGVVHDPRDGDVAVVSGSDNKGYFLRVGDKVYDGTVIAIDSSRGSMTFRQE
                     IDDPRRIKPYRDIVKRLVPLEDEESGDE"
     gene            3810..>5784
                     /gene="pilQ"
                     /locus_tag="VD788_05150"
     CDS             3810..>5784
                     /gene="pilQ"
                     /locus_tag="VD788_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HXV75687.1"
                     /translation="MSKQWKWTLAAAVSLALFVVASPALSGWASPDHSAETNWAELAR
                     LPERNLLDLRIDAGDEGLPTVTLDADGEVEFESFVLDGPDRLVIDLPGVVNRLETQRF
                     GVEQGGVVRIRAGQHTLEPAPVVRVVFDLVEPIPYRIDQVGSSIVVVFGDRRDDRSAS
                     TTEGSEPASAVPAEPIVVRPDGAGDARPAAVEVPTAQAEAATSESADEIAAELEARVP
                     EPGPEPVAAEATTTTSSSRSVDPDAIDELLNAPSFAAPQQQPDSSTRTFSSQRITTDQ
                     VTYRGKRISLNLVDADIKQVFRLFHEISGLNFVLDPDVAGRVTIVLDQVPWDQALDII
                     LKNNGLDKVLDNNVLRIATTNKLAMEAASRKRLKEAEELEVEPVTLTRTLSYARSRDV
                     EQVIRQGGVLSDRGRVIVDERTNTLIISDIPKKVEPLDALIDTLDAETPQVMIEARIV
                     ETSKQYARDLGIIWGFEAQANASHGTSTGINALHNTTLRYGLNLPGDGGASQLGFSFG
                     NIVDSFTLDLALNALETEGRGRIISAPKIATQNNERAEIEQGVRIPVTNTTATEIQVE
                     FVSASLLLSVTPQITAEGTIVLDVTVENNSPDFVNTAGGIPSIRTQRAQTKVLISDGG
                     TTVIGGIFVVNEGDSEVGVPWFRKLPVFGWLFRQ"
BASE COUNT         1144 a         1805 c         1888 g          947 t
ORIGIN      
        1 cactcgacga actgtcccaa cgtgatccag ctgatgtacg accccgagcg ccggatcgac
       61 gtcgactgga gcaccggcca ggaggacggc gaggcgctgt tcgacgtcaa gctgatgctc
      121 gacgtcgaga accgccaggg cctgttggcc aagatcgtct cgacggtggc cgacgagaag
      181 atcaacatca agaacgtcga agccgatgcg ttcgagttgg ccgacgcgaa gatccagatc
      241 gtgctttcgg tatcggatcg caagcagatg gaccgcgtga tccgcagcat ccgccggatc
      301 aagggtgtgc gcgacgtcga acgggtccgg cgctagcgct acctagacgg tcagctgacg
      361 gccttttcga ccttcccgct tcgcaggcag cgggtgcaga tgcgaacgcg cttgcggccg
      421 ccgccctggg tgcggacccg gacccgtcgg aggttcggca gccaacgccg gctcgtcaca
      481 ttgtgggcgt gggagacgtt gttgccgaac accgtctttt tgccgcagat tccgcagatt
      541 ctggccatcg tccccaccct gcgcccgtct ctcggccggt cgccgaaacg cgcatggtag
      601 acgggcgccc caggcgtgtc aaggcggagg acgggacgga agcgatcggt attcggcgcg
      661 gctcgcgccg ggcggagtat tcgtacttcc gctgacacat attgaatccg gcccggtcgg
      721 ggggttgcct tcggcgattc cgtctctata ttttccccca cgacgagaac ctggaagaac
      781 cggacaacgc cgaaacgagt ggcgttgggt ggttcgactg cgagggttcc ggcggaggag
      841 gttgggttgc aatacagcac acattccgtg gcggaaactg cttccggttc caaaaggaat
      901 gtgggttgtt tttgcttgac ttacgcgaaa tcgttgactt aggttgagcc cacgtttcag
      961 ggccgcctgg gcggggattt cgagtacccg agaggatacg ccatgctctt cggacgcacc
     1021 cggaacctgg tcggactgga tatcggggac agctcggtca aggtggtcga gctgaaggaa
     1081 ctgggcaaag ggaagggata ccaattgctc agcctggggt gggaacccct gtccgccgaa
     1141 gcgatcgtcg acggccagat catggattcc cagttggtcg cggaaaccgt ccaacgcctg
     1201 tgtcagcgct gccgcatcaa gtctggcagc caggtttcgg tcgccctgtc gggccaccac
     1261 gtcatcgtca agcgcatcag cctgccccag atgagcgagg ccgagctggc cgaatcgatc
     1321 cactgggaag ccgagcaata catcccgttc gacatcgacg acgtgaacct ggactaccag
     1381 attctcgaag gttcgagcct ctccggcgag ggcggcaaca tggacgtcct gctcgccgcg
     1441 gccaagacag acaagatcaa cgattacgtg agcgtcgtca gcgatgccgg cttgaacgcg
     1501 gtaaccgtcg acatcgcctc attcgcaatg cagaacgcgt tcgaggccaa ctacgaattc
     1561 gatccggtgc acgtgatcgc gctggtcgac atcggctcgg ccgtttcgtc gatctcggtg
     1621 ctgcacggtg gcacctcggt ctactggcgc gacatcaata tcggcggcaa ccagtatacc
     1681 gacgcgatcc agaaggagct gaacctgtcg gccgaccagg ccgagcagct caaacgaggc
     1741 gaggagatcg acggcatccc gtacgagcgc gtgctgccga tcctgtcgtc ggtcagcgac
     1801 gacatcggta ccgagatcca gaagaccctc gacttcttca agcagatctc ggccaccgac
     1861 cggccgctgg accggctcta cctgaccggc ggtacggcgc aggtcgtgca catgaaggaa
     1921 tcgctcggcg agcggctcgg aacgcaggtc gagctgttga acccgttccg caagatcccg
     1981 ccggcgggcc gggatgcttc gccggagctg gtgaacgaga tgatgccgac cgcgtcggtc
     2041 gccgtcggcc tggcgctgcg caagttcgga gactgacgcg atgatcaaga tcaacctgct
     2101 ggccgaaaag aaacagccca agcccgaacg cgcctcgacg atcaaggtcg agatgggcgg
     2161 tggacagaac ttcctgctgg tcgggatcct gctggtcggg gtcgtcatcg ccggcttctg
     2221 gtggtggtcg gcgagcagcg aactcgagga ctggaagcgc aagcatgtcg aggccgatgc
     2281 cgagctgcag cggctcgaag cgattcgcgc caagggcgag gagtacaaga agaagaaaga
     2341 agagctggcg cgcaagatcg acctgatcac gcggctgaag aagcaacagg ccgtgccggt
     2401 gcacatcctc gatcagatct cgaagaacct gcccgacttc ctctggttgg agtcgatgtc
     2461 ggtcgcgaac aacaacatcg tgatcaacgg caaggcgacg agctacaacg cggtcaccaa
     2521 tttctacaac aacctgggcg actcgggata cttcggcagc gtgaccttgg gccgggtgtt
     2581 cgaggtcaaa gagggagtcg cgttcgggat cacctgcagc ttcgccggct tgaagaccga
     2641 gccggccgtg ctaccgcaga actgacgggg agttggccat gttcgaaaga gttccattct
     2701 acgggcaggt gctgatcttc ctcgccatgg cggcgggcat cgtgctggtg gcctacaccg
     2761 tctaccccaa cctcggggcg atgcaggacg agatcacgca gctggaaaac gactacgccg
     2821 tcaaggaggc gaagatccgc gagggacagg cgatcgagca gcggttgccc gagttcgagc
     2881 gcgagatcga gacgctgcag cgcaaactgg gcgacatcca gcagatcctg ccgaccaacc
     2941 aggaaaccgg ggatctattg gcctggatca agaacgtcgg cgaccagtcg aatctcgatc
     3001 tgaagtcgtt ctctccgggc ggtctcaagc cggtcgactt ctacaaggag ttcccgatcg
     3061 agatgcaggt ggtcacgcgt tatcacgatc tcgggatctt cctcgacaag gtcagcaagt
     3121 actcccgcat catcaacgtc gacaacctga gaatcaacca atcgagtgag cgcgacaaga
     3181 cgatcagcgc cagtttcacg gcgaccacgt tcgtctacga cgaaaacgcg aaccctcagg
     3241 gaggcgtcca atgaaccgac ggcgattgat ctggtttgcc accatcgtga cgctgacgct
     3301 ggtggtcgcg gtctcgtcac cgggtttcat cgcccaatcg gtctcggccg cgaccgagga
     3361 gcagccgacc gagccggccg gcgagaccgg cgagaccggc gaggaagcgg tggagcagat
     3421 cctgcgtcag caggaggcgc tgatcaccgg acagggcttc tcctacgacc ccgagggtcg
     3481 tcgcgacccc tttcgctcgc cgttcggcgc gatcggcaac accgagcgca agcggccacc
     3541 gggaatggcc ggtatgctcg tcgccgaaat cgatctgacc ggagtcgtcc acgatccgcg
     3601 cgacggcgac gtcgcggtgg tcagcggttc ggacaacaag ggctacttcc tacgcgtggg
     3661 agacaaggtt tacgacggca cggtcatcgc catcgactcc tcccgcggct cgatgacttt
     3721 ccgccaagaa atcgacgatc cgcgaagaat caaaccctat cgagacatag tgaagaggct
     3781 ggtcccgctg gaggacgagg agagcggcga tgagtaagca gtggaagtgg acgctggcgg
     3841 cagccgtgag cctggcgctg ttcgttgtgg cctcgcccgc gctgagcggc tgggcctcgc
     3901 ccgaccacag cgccgagacg aattgggccg aactggcgcg ccttcccgag cgcaacctgc
     3961 tagatctgcg gatcgacgcc ggcgacgagg gactgccgac ggtcaccctc gacgcggacg
     4021 gcgaggtcga gttcgagagt ttcgtgctcg acggtcccga tcggctggtg atcgacctgc
     4081 cgggcgtcgt caaccggttg gagacccagc gcttcggcgt cgagcagggc ggcgtggttc
     4141 gcatccgcgc gggccagcac acgctcgagc ccgcaccggt cgtgcgcgtc gtcttcgacc
     4201 tggtcgagcc gattccctat cgcatcgatc aggtcggaag ctcgatcgtc gtcgtcttcg
     4261 gcgaccgacg cgacgaccga tcggcctcga cgaccgaagg gtccgaaccg gcgtcagccg
     4321 tcccggcgga gccgatcgtg gtccggcccg acggtgcggg tgatgcgcgg cccgcggcgg
     4381 tcgaggttcc cacggcgcaa gccgaggccg cgacgagcga atcggccgac gagatcgccg
     4441 ccgagctgga agcgcgcgtg ccggagccgg ggcccgagcc ggtcgcggcc gaagcgacga
     4501 cgaccacgag cagcagccgc tcggtcgacc cggacgcgat cgacgagcta ctgaacgccc
     4561 cgagcttcgc cgccccacag cagcaaccgg actcgagcac tcggaccttt tcgagccagc
     4621 gcatcacgac cgaccaggtg acctatcgtg gcaagcggat ctcgctcaac ctggtcgacg
     4681 ccgacatcaa gcaggtcttc cgcctgtttc acgagatctc gggtctcaac ttcgtgctgg
     4741 accccgacgt cgccggccgc gtgacgatcg tgctggacca ggtgccgtgg gatcaggcgc
     4801 tcgacatcat cctgaaaaac aacggactcg acaaggtgct cgacaacaac gtcctgcgaa
     4861 tcgcgacgac gaacaagctg gcgatggagg ccgcgtcgcg caagcggttg aaggaggccg
     4921 aggagctcga ggtcgaaccg gttacgctga cacgcacgct gtcctacgcc aggtcgcgcg
     4981 acgtcgaaca ggtgattcgc cagggcggcg tgttgtccga ccgcggccgc gtcatcgtcg
     5041 acgagcggac gaacacgctg atcatcagcg acatcccgaa gaaggtcgag ccgctcgatg
     5101 cgctgatcga cacgctcgat gccgagactc cgcaggtgat gatcgaggcg cggatcgtcg
     5161 agacgtcgaa gcagtacgcg cgcgacctcg gcatcatttg gggcttcgaa gcccaggcca
     5221 acgcgtctca cgggaccagc accgggatca acgcgttgca caatacgacg ctgcgctacg
     5281 ggctcaacct gccgggcgac ggcggagcca gccagctcgg cttcagcttc ggtaacatcg
     5341 tcgactcgtt cacgctcgat cttgcgctga acgcgctcga gaccgagggt cgtggacgga
     5401 tcatctcggc accgaagatc gccacgcaga acaacgagcg cgcggagatc gagcagggcg
     5461 ttcgcatccc cgtgaccaac accaccgcca ccgagattca ggtcgagttc gtctccgcgt
     5521 cgttgctgct gtcggtgacc ccgcagatca ccgccgaggg cacgatcgtg ctcgacgtga
     5581 cggtcgagaa caactcgccc gatttcgtca acacggccgg cgggattccg tcgatccgca
     5641 cccaacgcgc gcagaccaag gtgctgatct ccgacggcgg gacgacggtg atcggcggca
     5701 ttttcgtcgt caacgagggc gacagcgagg tcggcgtgcc gtggttccgc aagctgccgg
     5761 tcttcggctg gctgttccgc cagc
//
LOCUS       DASPGN010000179         2613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1433929, whole genome shotgun sequence.
ACCESSION   DASPGN010000179 DASPGN010000000
VERSION     DASPGN010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433929"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VD788_05155"
     CDS             <1..867
                     /locus_tag="VD788_05155"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXV75688.1"
                     /translation="GCVIQQPYDLEVGAGTMHPDTFFRVLGPEPWKVAYCQPSRRPVD
                     GRFGENPMRVYKHYQFQVILKPSPLDVQEQYLASLRAFGIDPLEHDVRFEEDNWESPT
                     LGAAGVGWQVLMDGMEISQFTYFQQAGGRELAPISVELTYGIERICMFLNDIRNIFDI
                     PWNDELTYGDVRHEEEVEHSRYSFDEAAVDVLARQFEQWEAEARRLVQPGDGPAAPDR
                     LLVLPAYEAVLKCSHLFNVLDARGALSVTERAASIQRIRRLACLCADAYLAKRERRGF
                     PLLARPAQRGSA"
     gene            864..>2613
                     /gene="glyS"
                     /locus_tag="VD788_05160"
     CDS             864..>2613
                     /gene="glyS"
                     /locus_tag="VD788_05160"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HXV75689.1"
                     /translation="MTSERRSFLFEIGCEEIPSRMIPPAADDLGRRIVEILDRAELGH
                     GLHAVWGGTRRLAVRVDDVAARQADRDETVLGPPAAVAFSADGELTQAALGFARKQGV
                     AVDRLERLDTERGSYVGLRRRVDGRPTAAILAETVPAAVAAMPFPKSMRWGDGRPRWV
                     RPVHWIVAIFGGETVPIELFGIRATTRSRGHRFFDDRWVEIERPDAYVDALRRAHVLI
                     EPAERRERIETALAARAAELGGTPVPDLALLDEVADLIEWPGVVAGSFDARFLELPPE
                     LLITTLRHHQKCFSLRDDRGRLLPGFLAVGNTDRDPAGHIRRGNEWVVGGRLEDARFF
                     WREDRKTALAALSPNLAGVTFHRKLGSYADKAERVSSLAVALAGSLGCDESELEACRR
                     AASWCKNDLVTGTVGEFPELQGRVGGLLLAAEGGAESVWRAIYQHYQPAGAEDEVPSG
                     TVAQVVSVADKLDSVCRLIEAGERASGSKDPLGLRRAGSGIVRVLLDGGWPITLAELA
                     ELARADEAAGAFLRERMESVLRERGHSIKEILSVTRARVDPERALAWSMIDLAARLDA
                     IKRVRGRDDFVHLVKLT"
BASE COUNT          376 a          867 c          947 g          423 t
ORIGIN      
        1 ggatgcgtga tccagcagcc ctacgacctg gaagtcggcg ccggaacgat gcatcccgac
       61 acgttcttcc gcgtgctcgg cccggagccg tggaaggtcg cctactgtca gccgtcgcgg
      121 cgtccggtcg acggtcggtt cggcgagaac ccgatgcgcg tctacaagca ctatcagttt
      181 caggtgatcc tcaagccatc gccgctcgac gtgcaggagc agtacctcgc ctcgttgcgc
      241 gcgttcggga tcgacccgct cgagcatgac gtgcgtttcg aagaggacaa ctgggagtct
      301 ccgactctcg gggcggcagg tgtcggctgg caggtactga tggacgggat ggagatctcg
      361 cagttcacct acttccagca ggccggtggg cgcgagcttg ctccgatctc ggtcgagctg
      421 acctacggga tcgagcgcat ctgtatgttt ctcaacgaca ttcggaacat cttcgacatc
      481 ccgtggaacg acgagttgac ctacggcgac gtccgtcacg aggaggaggt cgagcactcg
      541 cgctactcgt tcgacgaagc cgccgtcgac gtgctcgcac gccagttcga gcagtgggag
      601 gccgaggcgc gccggttggt ccagccgggg gacggccccg ccgcgccgga ccgactgctc
      661 gtgctgccgg cctacgaggc ggtgctgaag tgctcccatc tgttcaacgt gctcgatgcg
      721 cgcggggcgc tcagcgtgac cgaacgtgcc gccagcatcc aacgcatccg tcgtttggct
      781 tgcctgtgcg ccgacgccta cctggcgaaa cgcgagcgtc gaggttttcc gctgctggcg
      841 cggccggccc aacggggttc cgcgtgacgt cggagcgccg gtccttcctg ttcgaaatcg
      901 gctgcgagga gatcccgtcg cggatgatcc cgccggcggc cgacgacctc ggtcgccgga
      961 tcgtcgagat cctcgaccgt gcggagctgg gacacggact gcatgccgtc tggggcggca
     1021 cccgccgact ggcggtccgc gtcgacgacg tcgcggcgcg gcaagcggac cgcgacgaga
     1081 cggtcctcgg tcccccggcc gcggtcgcgt tctcggccga cggcgagttg acccaggcgg
     1141 cgctcggttt cgcccgcaag cagggtgtcg ccgtcgaccg gttggagcgt ctcgacaccg
     1201 agcgcggcag ctatgtcggc ctgcgccggc gggtcgacgg ccggccgacg gccgcgatcc
     1261 tcgccgagac ggtgccggcg gcggtggcgg cgatgccgtt tcccaagtcg atgcgctggg
     1321 gcgatggaag accgcgctgg gtccggccgg tccactggat cgtggcgatt ttcggcggcg
     1381 agacggtgcc gatcgaactg ttcggaatcc gcgcgacgac ccgctcgcgc gggcacaggt
     1441 tcttcgacga ccgctgggtc gagatcgagc gacccgatgc gtacgtcgac gcgttgcgcc
     1501 gtgcacacgt gctgatcgag ccggcggagc ggcgcgagcg gatcgagacc gcgctcgccg
     1561 ctcgggccgc ggagctcggg ggaactccgg tgccggatct ggcgttgctc gacgaggtcg
     1621 ccgacctgat cgaatggccg ggcgtcgtcg ccggcagctt cgacgcgcga ttcctcgagc
     1681 ttccgcccga gctgttgatc accacgctgc gtcaccatca gaagtgcttc tcgttacgag
     1741 acgaccgcgg ccggttgctt ccgggctttc tcgccgtcgg caacaccgat cgcgatcccg
     1801 cgggccacat ccgtcgcggc aacgaatggg tcgtcggcgg tcgactcgag gacgcgcgct
     1861 tcttttggcg cgaggatcgc aagacggcgc tcgccgcgct gtcgcccaac ctcgcggggg
     1921 tgacgttcca ccgcaagctg ggcagctacg ccgacaaggc ggaacgcgtt tcgagcttgg
     1981 ccgtcgcgct ggccggcagc ctcggctgcg acgagtcgga gctcgaggcc tgccgccgcg
     2041 cggcgagttg gtgcaaaaac gacctggtga cgggcaccgt cggagagttc cccgaattgc
     2101 agggacgggt cggcgggctg ctgctggccg ccgagggggg cgccgagtcg gtgtggcgcg
     2161 cgatctacca gcactaccag ccggccggcg cggaggacga ggttccgagc ggcaccgtcg
     2221 cgcaggtcgt ctccgtcgcc gacaagctcg acagcgtctg ccgcttgatc gaggccggcg
     2281 aacgagccag cggctcgaaa gacccgttgg ggctgcgtcg ggcgggcagc gggatcgtcc
     2341 gcgtgctgct cgacgggggc tggccgatca cgctggccga gctggccgag ctggcgcgag
     2401 ccgacgaagc cgccggcgcc ttcctccgcg agcgaatgga gagcgtgctg cgcgagcgcg
     2461 gtcactcgat caaggagatt ctctcggtca cgcgggcgcg ggtcgacccc gagcgggcgc
     2521 tcgcgtggtc gatgatcgac ctcgcggcgc ggctcgacgc gatcaagcgc gttcgtgggc
     2581 gcgacgattt cgtccacttg gtcaagttga ccc
//
LOCUS       DASPGN010000180         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1668400, whole genome shotgun sequence.
ACCESSION   DASPGN010000180 DASPGN010000000
VERSION     DASPGN010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1668400"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VD788_05165"
     CDS             complement(<1..463)
                     /locus_tag="VD788_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75690.1"
                     /translation="MLTQLLRNLGLARHLGEPVIVVSGLPRSGTSMMMNMLTAAELEV
                     VSDRVRVADEDNPKGYFEDERVKDLERNPDRAWLRDCRGKVVKIVSFLLKDLPDDNRY
                     KVIFMRRDLGEVIASQNKMLERRNEERGSGDMNEDEKMIRLYETHLRKIDFL"
     gene            complement(536..964)
                     /locus_tag="VD788_05170"
     CDS             complement(536..964)
                     /locus_tag="VD788_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HXV75691.1"
                     /translation="MTDRADDNVVSSRAPEPVGAFPHAKRVGSLLFLSGIGPRVRGRN
                     EIPGVRLGSRGEVASYDIEQQCRSVFRNVRTVLEDAGSAWERIVDVTVFLTDIERDFP
                     TFNRLYAEHFADNQPARTTVEVNRLPTPIAIELKVIATVG"
     gene            complement(981..2435)
                     /locus_tag="VD788_05175"
     CDS             complement(981..2435)
                     /locus_tag="VD788_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HXV75692.1"
                     /translation="MLRVPNFIDGEFAPPAGGEWLDNVEPATGEVYSQVAGGGRADVD
                     AAVAAAERAAAGWAATPAAERARLLEAIAARIESDLERFARAESIDSGKPIAVARTVD
                     IPRAASNFRFFASAVLHWSSEAHPMDTRALNYTLRRPRGVAGLISPWNLPLYLSSWKI
                     APALATGNTAVAKPSELTPMTAHLLAEVCREVGLPAGVLNVVHGRGADAGASLVAHPG
                     VATLSFTGGTATGREIARSAAPLFKKIALEMGGKNPNIVFADARMDRAVATATRAAFS
                     NQGQICLCASRIFVERSAFGEFVERLVAAADALDVGDPLDERTEQGALVSRAHLDKVR
                     SYVELAREEGGTIRSGGGPPAELPRRVRNGYFLRPTVITGLDAACRVNQEEIFGPVVT
                     VMPFDDEREVIEQANGTPYGLAATLWTENLSRAHRLAERIGSGTVWVNCWMLRDLRVP
                     FGGMKSSGVGREGGEEALRFFTEPKNVCVEWPEG"
BASE COUNT          393 a          930 c          897 g          398 t
ORIGIN      
        1 gcaggaagtc gatcttccgc aggtgcgtct cgtagaggcg gatcatcttc tcgtcctcgt
       61 tcatgtcccc gctgccccgc tcctcgttgc gtcgctcgag catcttgttc tgcgaggcga
      121 tcacctcgcc gaggtcgcgt cgcatgaaga tcaccttgta gcggttgtcg tcgggcaggt
      181 ctttgagcaa gaacgagacg atcttgacca ccttgccccg gcagtcgcgc agccaggcgc
      241 ggtccgggtt ccgctcgaga tccttgaccc gctcgtcctc gaagtagccc ttcgggttgt
      301 cctcgtcggc gacccgcacg cggtcggaga ccacctcgag ctcggccgcg gtcagcatgt
      361 tcatcatcat cgaggtgccc gagcgcggca ggccggagac gacgatcacc ggctcgccga
      421 ggtgcctggc gaggccgagg tttcgcagca gctgggtcag caaggaccat ccctcgacag
      481 caaaagtaca gcctataccg gatcacggcc ggcggccagg tccgatccgc gaccgtcagc
      541 cgacggtggc gatcaccttg agctcgatcg cgatcggcgt cgggagccga ttgacctcga
      601 ccgtcgtgcg cgccggttga ttgtcggcga agtgctcggc gtacaaccga ttgaacgtcg
      661 gaaagtcacg ctcgatgtcc gtcaggaaga ccgtcacgtc gacgatccgc tcccaggcgc
      721 tgccggcgtc ctccagcacg gttcgcacgt tgcgaaaaac cgagcggcac tgctgctcga
      781 tgtcgtagga cgcgacctcg ccgcgcgagc cgaggcgtac gccgggaatc tcgttccggc
      841 cccgcacgcg cgggccaatg ccgctgagga acagcaggct gcccacccgt ttggcgtggg
      901 ggaaggcgcc gaccggctcg ggcgccctgc tgctgaccac gttgtcgtcc gcacgatcgg
      961 tcatcgatcc gcctcccgtc ctagccctcg ggccactcga cacagacgtt cttcggctcg
     1021 gtgaagaacc gcagcgcttc ctcgccgccc tcgcgaccga cgccgctcga cttcatcccg
     1081 ccgaacggca cgcgcagatc gcgcagcatc cagcaattga cccagaccgt gcccgagccg
     1141 atccgctcgg cgaggcggtg ggcgcgcgac aggttctcgg tccacagcgt cgcggcgagc
     1201 ccgtacggcg taccgttggc ctgctcgatc acctcgcgct cgtcgtcgaa cggcatcacg
     1261 gtgacgaccg gtccgaagat ctcctcctgg ttgacccggc aggccgcgtc gagaccggtg
     1321 atcacggtcg ggcgcaggaa gtagccgttc ctcacccggc gcggcagctc ggccggcgga
     1381 ccaccgcccg agcggatcgt cccgccttcc tcgcgcgcga gctcgacgta cgagcgcacc
     1441 ttgtcgagat gggcccgcga gacgagcgcg ccctgctccg tacgctcgtc gagcgggtcg
     1501 cccacgtcga gcgcatcggc cgcggcgacg aggcgctcga cgaactcgcc gaaggcgctg
     1561 cgctcgacga agatgcgcga ggcgcaaaga cagatctgac cctggttgga gaaggccgca
     1621 cgggtggcgg tcgcgaccgc gcggtccatc ctcgcgtcgg cgaagacgat gttcgggttc
     1681 ttgccgccca tctcgagcgc gatcttcttg aacagcggcg ccgccgaccg cgcgatctcg
     1741 cgcccggtcg cggtgccacc ggtgaacgac agcgtcgcca caccgggatg tgccacgagc
     1801 gacgcgccgg cgtcggcgcc ccgtccgtgc acgacgttga gcacgccggc gggtagcccg
     1861 acctcgcgac agacctcggc cagcaggtgc gcggtcatcg gcgtcagctc ggacggcttg
     1921 gccaccgcgg tgttgccggt cgccagcgcg ggggcgatct tccagctcga caagtacaac
     1981 ggcagattcc acggcgagat cagaccggcc acgccccgcg ggcgacgcag cgtgtagttc
     2041 agcgcgcgcg tgtccatcgg gtgggcctcg ctgctccagt gcagcacggc cgacgcgaag
     2101 aagcggaagt tgctggcggc gcgcgggatg tcgaccgtac gggcgacggc gatcggtttt
     2161 ccgctgtcga tcgactcggc gcgggcgaat cgttcgaggt cgctttcgat ccgtgcggcg
     2221 atcgcctcga gcaggcgcgc gcgctcggcg gccggcgtcg cggcccagcc ggccgcggcg
     2281 cgctcggccg ccgccaccgc cgcgtcgacg tcggctcggc cgccgcccgc cacctgcgag
     2341 tagacttcgc ccgtcgccgg ctcgacgttg tcgagccact cgccgccggc aggcggcgcg
     2401 aactcgccgt cgatgaaatt cggcacgcgc agcatgcgtg gcgattctag cgcatcgggg
     2461 ccggtatcat tcgacggcgg caaccagccg cgggctcgcg ggcggcaggt cggctggccg
     2521 gacgcggcgc gggtggcgga cgagccgctc ggagcgcagc ccgtcggcga gatagtaaag
     2581 agcgatcacc gtcgccgcga tcgcgagccc gggaagcg
//
LOCUS       DASPGN010000181         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_145986, whole genome shotgun sequence.
ACCESSION   DASPGN010000181 DASPGN010000000
VERSION     DASPGN010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145986"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(488..1966)
                     /locus_tag="VD788_05180"
     CDS             complement(488..1966)
                     /locus_tag="VD788_05180"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV75693.1"
                     /translation="MERAQILVADDEREVRDVYARVFSSLGHDVAAAVDGEQVVEQVR
                     DRVFDLVLCDIVFPPTDGIDVLRSVKRVRPSTPVVLFTGHASVDRVLDAFRAGAFDVL
                     EKPIPHERLKVLADRAIELRKMGDSRRRLAEDLEHERMKVIDMRRRLALDDPFCKIIG
                     RSGLLTDFVDTIREVARTDSTVLLTGESGTGKSLVARTIHQASARAEGPFVEANCVVY
                     SEGVLQSELFGHEKGAFTGAERQKKGRFELADRGTLFLDEIGEIAPSIQLMLLRVLQE
                     RNFERVGGERTIEANVRLIAATNRDLEEAIRQNTFRSDLYYRLNVIPIHLPALRDHAE
                     DIPVLAQHFTVRSAGALGRETPEFSEQAMEALTHYGWPGNVRELENVVERVVVLARTG
                     RVELRDLPEGVRAAAAGNGARVGSGTLQDVERARILDVLDQTGGNKRLAARRLGIHRS
                     TLYAKLRRYGLLDRIAAAGGDRTVERTESRQPVSGSPTTRGN"
     gene            complement(2172..2249)
                     /locus_tag="VD788_05185"
     tRNA            complement(2172..2249)
                     /locus_tag="VD788_05185"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2211..2213),aa:Glu,seq:ttc)
BASE COUNT          380 a          828 c          836 g          396 t
ORIGIN      
        1 acgacgtcgg gcagccgttc gcgggccagt gcgagcgccg tggccccgtc tgcggcggtc
       61 cagaccgtgt agccctcggc gcgcagcagt ccttcgagca gcttgcggct ccggtcgtcg
      121 tcgtcgacga cgagcatccg cgccgtcgtc ggttggtgtc gttcgctcat gcccacgccc
      181 ccggctaccg acggtaatcc cgacttcgaa tctaggtttc ccgttcggcc gaggcacgcc
      241 ggcccgccgc gatcgcgaaa aaaaacgggg cgctcacgag agcgccccga gcgacagagg
      301 tagttggggg ggcgagtgat tcgtcgtttc gctcggagga gacagcaagg cgagtgccag
      361 ccgtcacggt gcgcaagtca cgggcttttc gtgcggcggg tggaccgtcg ccgggtttcg
      421 tgtccgatcc ggcgaccacg acgaaccgcc ggcccgccgg tcgaggaccg caactcgttg
      481 acccgtgtca gtttccgcga gtggtcggag aacccgacac gggctgtcgg ctttccgtcc
      541 gctcgacggt ccgatcgccg ccggcggcgg cgatgcggtc gagcagcccg taacggcgca
      601 gcttggcgta gagcgtgctg cggtggatac cgagccgacg agcggccagt ctcttgttgc
      661 cgccggtctg gtcgaggacg tcgaggatcc gggcccgctc gacgtcctgg agcgtgccgc
      721 tccccacgcg ggcaccgttg ccggccgccg cggcgcggac gccctcgggc aggtcgcgca
      781 gctcgacgcg tccggtgcgc gccagcacga cgacgcgctc gacgacgttc tcgagctccc
      841 gcacgttgcc cggccagccg tagtgtgtca gcgcctccat cgcctgctcg ctgaactcgg
      901 gtgtctcgcg gcccagggca ccggcggagc gcacggtgaa gtgctgggcc agcaccggga
      961 tgtcttcggc gtggtcgcgc agcgcgggca ggtggatcgg gatcacgttg agccggtagt
     1021 acaggtcgct gcggaaggtg ttctggcgga tcgcctcctc taggtcgcgg ttcgtcgcgg
     1081 caatcagccg cacgttggcc tcgatcgtcc gttcgccccc gacccgctcg aagttccgtt
     1141 cctgcagcac gcgcagcaac atcagctgga tcgacggcgc gatctcaccg atttcgtcga
     1201 ggaacagcgt gccacgatcg gccagctcga accgcccttt cttctgacgc tccgcgccgg
     1261 tgaaggcacc cttttcgtgg ccgaacagct cgctctgcag cacgccctcg gagtagacga
     1321 cacagttggc ctcgacgaac ggtccctcgg cgcgcgccga cgcttgatgg atcgtccgcg
     1381 ccacgagact cttgccggta ccgctctcgc cggtcaacag cacggtcgaa tcggtccgcg
     1441 cgacctcgcg gatcgtgtcg acgaagtcgg tcaacaggcc ggaccgaccg atgatcttgc
     1501 agaacggatc gtcgagcgcc agccggcgcc tcatgtcgat caccttcatc cgctcgtgct
     1561 cgagatcctc ggccagccgg cgacggctgt cgcccatctt acggagctcg atcgcgcgat
     1621 cggcgagcac cttgagccgc tcgtgcggga tcggtttctc cagcacgtcg aatgcgccgg
     1681 cccgaaacgc gtcgagcacg cgatcgacgg aggcatgacc ggtaaacaac acgaccggcg
     1741 tcgacggccg cacccgtttg accgaacgca gcacgtcgat cccgtcggtc ggcgggaaga
     1801 cgatgtcaca cagcacgaga tcgaagacgc gatcgcggac ctgctcgacg acctgctcgc
     1861 cgtcgaccgc agcggcgacg tcgtgaccga gcgacgagaa gactcgggcg tagacgtcgc
     1921 ggacctcgcg ttcgtcgtcg gccacgagga tctgtgcgcg ttccatgccg gacaccctcc
     1981 acacggtcga gcggtgcgcc gccggccgtg tcgcgaggcg gctgcaagat cggttccgtc
     2041 tcgtcaccgc gccgaaaggc ggagccggag ccgattcgac cgagatcggc gggtcgcgac
     2101 cgccaacgat tgtcagccag gggacacgcg ctgtcgcagt ttcggtcact ggaaccggcg
     2161 gcggatgcgg ttggcgtccc caacgggatt cgaacccgtg tcgccacctt gaaagggtgg
     2221 tgtcctaggc cagactagac gatggggacg gaagatcgct tcggagccgg gaagtctagc
     2281 ggaacgattc ggcgcgggcc atccgcgacc ggccgtcacg gcccgggggc ggcaggagtg
     2341 caacggctct cgcgtttcct gggcgaatcc gtggtgagcc gtgctggatt cgaaccagcg
     2401 accctcagct taaaaggctg gtgctctacc tggctgagct
//
LOCUS       DASPGN010000182         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_380222, whole genome shotgun sequence.
ACCESSION   DASPGN010000182 DASPGN010000000
VERSION     DASPGN010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380222"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2119)
                     /locus_tag="VD788_05190"
     CDS             complement(<1..>2119)
                     /locus_tag="VD788_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75694.1"
                     /translation="VGIDLTLPSTPTNWLQGGAIVINGVPFPCSTRCGTNNPGYALAG
                     LNLAPGAVRTISIPVLVPVGASGLGDVTIHMWNNQGGGGFGGAFETAFQKAATINVGA
                     VRTAKPTLDCPIPSSAVQISGTSEPDAAIKAKFSLIDRGTGTADGVGDWVVNNFGAFG
                     GLYGGLEVRVTATAPGKLESVLSDECFVTQTRECSDTVDNDGDGLTDFPADPGCTGPT
                     DNSELDEIPECSDTLDNDSDGPIDFPADLGCFAAGDPVEDLNPACSDGIDNDGDGLID
                     FSGPMPDPDCANANDIDELTRPQCQDGVDNDGDGDIDFPDDVFCNSAFDDDEFDISVP
                     SDIKARLLIVFDTSGSMNFNTCDATFTGGDGSTECPGFDVSCSTCPATDCNNGLPDDS
                     RMAKVKAGLNNVIAGFGEVEYGLMRFHQRGESFQIPEDCPSQNASLQSGGWQGGGAEP
                     CGGGFNAGDLLVSFSFDNQSDLLAWMDGDSNYPGEPPPNLDWELRGSGTTPIAGSLAS
                     ALTYLEAVEDDDPASGCRPYEVILVTDGAETCGGDPDAAAAALLTAGFPVNVIGFATS
                     DPTIVAGLDSIANAGGTGSAIIASDSTALSAAIADIIDDTILVEHCNSIDDDCDVLVD
                     EDFPTLGDTCDNGELGVCFQTGPVVCRTIADDGDDLGVKCDAPGGMPSTEICDGLDNN
                     CNGLVDEGFPVGCGCVPTPEVCNG"
BASE COUNT          290 a          729 c          757 g          343 t
ORIGIN      
        1 gccgttgcag acctccggcg tcgggacgca gccgcagccg accgggaagc cctcgtcgac
       61 gaggccgttg cagttgttgt cgagaccatc gcagatctcc gtgctcggca tgccgccggg
      121 cgcgtcgcac ttgacgccga ggtcgtcgcc gtcgtcggcg atcgtgcggc acacgaccgg
      181 gccggtctgg aagcacacgc ccagctcgcc gttgtcgcag gtgtcgccca gcgtcgggaa
      241 gtcctcgtcc acgagcacgt cgcagtcgtc gtcgatgctg ttgcagtgct cgaccaggat
      301 cgtgtcgtcg atgatgtcgg cgatcgccgc cgacagcgcg gtcgagtcgg acgcgatgat
      361 cgccgagccg gtgccgccgg cgttcgcgat cgagtccagg ccggcgacga tcgtcggatc
      421 gctggtggcg aacccgatca cgttgaccgg gaacccggcc gtcagcagcg ccgccgccgc
      481 cgcgtccgga tcgccgccgc aggtctcggc gccgtcggtc acgaggatga cctcgtacgg
      541 gcggcaaccc gacgcggggt cgtcatcctc cacggcctcg aggtacgtca gcgccgacgc
      601 cagcgagccc gcgatcggcg tcgtcccgct gccgcgcagc tcccagtcca ggttcggcgg
      661 gggctcgccc ggatagttgg agtcgccgtc catccaggcc agcaggtcgg actggttgtc
      721 gaacgagaag ctcacgagca ggtcgccggc gttgaacccg ccgccgcacg gctcggcgcc
      781 gccgccctgc cacccgccgg actgcaagct cgcgttctgc gacgggcagt cctcggggat
      841 ctggaacgac tcgccgcgct ggtggaagcg catcaggccg tactcgacct cgccgaaacc
      901 ggcgatgacg ttgttcaggc ccgccttgac cttggccatg cggctgtcgt ccggcaggcc
      961 gttgttgcag tcggtggccg ggcaggtcga gcacgacacg tcgaagccgg ggcactcggt
     1021 cgagccgtcg ccgccggtga acgtcgcgtc gcaggtgttg aagttcatcg agccggacgt
     1081 gtcgaacacg atcagcaggc gcgccttgat gtcggagggc acgctgatgt cgaactcgtc
     1141 gtcgtcgaac gccgagttgc agaacacgtc gtcggggaag tcgatgtcgc cgtcgccgtc
     1201 gttgtcgacg ccgtcctggc actgcggccg cgtcagctcg tcgatgtcgt tggcgttcgc
     1261 gcagtccgga tcgggcatcg ggccgctgaa gtcgatgagc ccgtcgccgt cgttgtcgat
     1321 gccgtcggag caggcggggt tgagatcctc gaccgggtcg ccggccgcga agcagcccag
     1381 gtccgccggg aagtcgatcg ggccgtcgct gtcgttgtcc agcgtgtccg agcactcggg
     1441 aatctcgtcg agctcgctgt tgtcggtcgg gccggtgcag cccgggtcgg ccgggaagtc
     1501 ggtgaggccg tcgccgtcgt tgtcgacggt gtccgagcac tcgcgcgtct gcgtgacgaa
     1561 gcactcgtcc gacaggaccg attcgagctt gccgggcgcg gtggcggtca cgcgcacctc
     1621 gaggccgccg tacaggcccc cgaacgcgcc gaagttgttg accacccagt cgccaacgcc
     1681 gtcggccgtc ccggtgccgc ggtcgatcaa gctgaacttc gccttgatcg ccgcgtcggg
     1741 ctcgctcgtg ccgctgatct ggaccgcgct cgacggaatc gggcagtcca gcgtcggctt
     1801 ggccgtgcgc accgcgccca cgttgatcgt cgccgccttc tggaacgcgg tctcgaacgc
     1861 gccgccgaag ccgccgccgc cctggttgtt ccacatgtgg atcgtgacgt cgccgaggcc
     1921 cgaggcgccg accggcacca ggaccggaat cgagatcgtg cgcaccgcgc cgggcgcgag
     1981 gttcaggccg gcgagcgcgt agccggggtt gttggtcccg caccgcgtcg agcaggggaa
     2041 cgggacgccg ttgatgacga tcgcgccgcc ctgcagccag ttcgtcggcg tgctcggcag
     2101 cgtcagatcg atgccgacg
//
LOCUS       DASPGN010000183         3249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_380381, whole genome shotgun sequence.
ACCESSION   DASPGN010000183 DASPGN010000000
VERSION     DASPGN010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380381"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1615)
                     /locus_tag="VD788_05195"
     CDS             complement(179..1615)
                     /locus_tag="VD788_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HXV75695.1"
                     /translation="MGVDEFRREARRVADRVADYLERLESFDVLPKVEPGVVGSRLPA
                     APPLEPEPMERILGDYHALIEPNVTHWQHPGFMAYFPSVASGPGILGEWLATGLNSNV
                     MFWRNAPASTELEQRVVDWMRRMLGLPEPFDGMFTDTASVSSMLSLVAARHAIPGLDS
                     RDEGLAGRDGLARPRLYVSTEAHMSIEKAAIVSGIGRAGVRHIPTDAEFRMRPDALDR
                     AIAEDRAAGWLPFCVVGTLGTTSSSSVDPPAALARICRREGLWLHLDAAYAGVAAIVP
                     EMRHHFDGWEQADSIVINPHKWMFTPFDASLLLFREADRFRDAFSLVPEYLKTGVGPS
                     VRNYNEFGVQLGRRFRALKIWFEIRYFGVDGIAARLREHCRMAREFAGWVAAAPDWEL
                     LAPVPFSTVCFRHRPPAAGDDERRLEAVNQAILDRVNRSGRVYLSHTKLRDRFTLRVV
                     VGNPRQTIDHVHGCWTILQQAARQATGS"
     gene            complement(1660..2190)
                     /locus_tag="VD788_05200"
     CDS             complement(1660..2190)
                     /locus_tag="VD788_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75696.1"
                     /translation="MREVFGVSLARWLPLVVLFAVASPAAAANRWWFGGGIGLSFGDV
                     DYVSIEPAIGYVPVDKVTIGTRLIYRHRDDDRFDPSVSSTDYGASLFGRYFVAPAVFL
                     QAEYEYLDYEFLRVDGSTDRDGYGSWLAGVGYSRPISSGASFFVLGLYNFSYADDEPS
                     PYSDRYVLRVGVGVAF"
     gene            complement(2183..2974)
                     /locus_tag="VD788_05205"
     CDS             complement(2183..2974)
                     /locus_tag="VD788_05205"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXV75697.1"
                     /translation="MFWVAVLLGIVEGLTEFLPISSTGHLIVTNRLLGFDGIRAEVFA
                     VFIQLGAILAVVWEYRAKLTRVTLGLARDAASRAFAGNLLLAFLPAAVIGFLIHDFIS
                     ARLFSPLTVALALIAGGLLIFVVEALPLPARTRDVDSVGWKQALGVGVAQCVAMWPGF
                     SRSAATILGGMIAGLDRRTATEFSFFLAIPTMFAATVYDLVKNYERLAPGDLSWLALS
                     FVISFLVAWASVRWLLRFVSTHSFRAFAWYRLAFGGIILLVLYRA"
     gene            complement(2982..>3249)
                     /locus_tag="VD788_05210"
     CDS             complement(2982..>3249)
                     /locus_tag="VD788_05210"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXV75698.1"
                     /translation="VGIDYSIHLVHRLTLSGGEVSDGLLETGKAVVMAALTNIAGFGT
                     LWLGNYPALRSFGQVALIGSITCLFTALTFVPAVMGRRPTAGSD"
BASE COUNT          599 a         1107 c         1117 g          426 t
ORIGIN      
        1 ctggcggcga tcgttcgcgc ggtcgggaac agagcaagag gcggaccagc gcgctcgcgc
       61 ggcggccgcg cgcgacaagc ggctgagccg acgggagttg cgtcaacagg ccggagaaaa
      121 agcgaacggt gggcgccggg aattcgtcga ggatctcatc ggccgccgtt gaaggcttct
      181 acgatccggt cgcttggcgt gccgcctgct gcaggatcgt ccaacagccg tgcacgtggt
      241 cgatcgtctg acgcgggttg ccgacgacca cccgcagcgt gaaccgatcg cggagcttgg
      301 tgtgcgacaa gtagacccgc ccgctgcgat tgacgcgatc caggatcgcc tggttgaccg
      361 cctcgagccg ccgttcgtcg tcgcccgcgg ccggcggccg gtggcggaag cagacggtgc
      421 tgaacgggac gggcgccaac agctcccaat ccggcgctgc cgcgacccaa ccggcgaact
      481 cgcgcgccat ccggcaatgc tcgcggagtc gtgcggcgat cccgtcgacg ccgaagtagc
      541 ggatctcgaa ccagatcttg agcgcgcgaa aacggcggcc gagctggacg ccgaactcgt
      601 tgtaattgcg caccgacggg ccgacgcccg tcttgaggta ctccggcacc aggctgaacg
      661 cgtcgcggaa gcggtccgcc tcgcggaaca gcagcaacga tgcgtcgaac ggggtgaaca
      721 tccacttgtg tgggttgatc acgatcgaat cggcctgctc ccagccgtcg aagtggtggc
      781 gcatctcggg tacgatcgcc gccaccccgg cgtaggccgc gtcgaggtgc agccacagcc
      841 cttcccggcg gcagatgcgc gccagcgccg ccggcgggtc gacgctgctc gacgaggtcg
      901 tgcccagcgt cccgacgacg cagaacggca gccaacccgc ggcgcggtct tccgcgatcg
      961 cgcggtcgag cgcgtcgggt cgcatgcgga actcggcgtc ggtcgggatg tgccggacgc
     1021 ccgcccggcc gattccgctg acgatcgcgg ccttctcgat cgacatgtgg gcctcggtcg
     1081 agacgtagag ccgcgggcgg gcgagcccgt cccgtccggc gagcccctcg tcacgcgagt
     1141 cgagcccggg gatcgcgtgg cgtgccgcga ccaacgacaa catcgacgac accgaggccg
     1201 tgtcggtgaa catgccgtcg aacggctcgg gaagaccgag catccgacgc atccaatcga
     1261 cgacgcgctg ctcgagctcg gtcgacgccg gcgcattgcg ccagaacatc acgttcgagt
     1321 tgagcccggt ggccagccat tcgccgagga tacccggccc gctggcgacg ctggggaagt
     1381 aggccatgaa gcccgggtgc tgccagtgcg tgacgttggg ttcgatcagc gcgtggtagt
     1441 cgccgaggat gcgttccatc ggttcgggtt cgagaggcgg tgccgcgggc agccgcgagc
     1501 cgaccactcc gggctcgacc ttcggcagca cgtcgaacga ctcgagccgt tcgaggtagt
     1561 cggccacccg gtccgccacg cggcgcgcct cgcggcggaa ctcgtcgacg cccatgtcgc
     1621 ccagcccgcg ctgggcgtcg ctgatttcgt catcgcgtgt cagaaggcga cccccacacc
     1681 cactcgcagc acgtaacggt cgctgtaggg cgacggttcg tcgtccgcgt acgagaaatt
     1741 gtagagcccg agcacgaaga acgaggcgcc ggacgagatc ggtcgagagt agccgacccc
     1801 ggcgagccag ctcccgtagc cgtcgcggtc ggtcgacccg tcgactcgca ggaactcgta
     1861 atcgagatac tcgtactcgg cctggaggaa gaccgccggg gcgacgaagt agcgaccgaa
     1921 caggctcgcg ccgtagtcgg tcgagctgac cgacggatcg aagcggtcat catcccgatg
     1981 gcggtagatc aagcgcgtgc cgatggtgac cttgtcgacg gggacgtagc cgatcgccgg
     2041 ttcgatcgag acgtagtcga cgtcgccgaa gctcagaccg atgcccccgc cgaaccacca
     2101 gcggttcgcc gccgcggcgg gcgacgccac ggcgaacagc acgacgagcg gcaaccaacg
     2161 tgccagcgat actccgaaaa cctcacgcac ggtacagcac cagcaggatg atcccaccga
     2221 acgccagccg ataccaggcg aaggcgcgga acgagtgcgt cgagacgaaa cgcagcagcc
     2281 agcggaccga ggcccaggcg acgagaaacg aaatgacgaa cgacagcgcc agccagctca
     2341 ggtcgccggg cgccagccgc tcgtagttct tgacgaggtc gtaaacggtg gccgcgaaca
     2401 tcgtcgggat cgcgaggaag aacgagaact cggtcgccgt gcggcgatcg agaccggcga
     2461 tcatcccgcc gaggatcgtc gcggccgacc gcgagaagcc cggccacatc gcgacgcatt
     2521 gcgccacgcc gacgccgagc gcctgcttcc agccgacgct gtcgacgtcg cgggtcctcg
     2581 ccggcagcgg cagcgcctcg acgacgaaga tcaacagccc gcccgcgatc agcgccagcg
     2641 ccaccgtcaa cggcgagaac aggcgcgcgg agatgaagtc gtggatcaag aacccgatca
     2701 cggccgccgg caggaaggcc agcagcaggt tcccggcgaa cgcgcgcgag gccgcgtcgc
     2761 gcgccaagcc gagggtcacg cgtgtcagct tggcccggta ctcccatacc accgccagga
     2821 tcgcgccgag ctggatgaac acggcgaaca cctccgcgcg gatgccgtcg aatccgagca
     2881 ggcggttggt cacgatcaag tgtccggtcg acgagatcgg caggaactcg gtcagccctt
     2941 cgacgatgcc gaggagcacc gcgacccaga acatctgcaa ctcagtcgct ccccgccgtc
     3001 ggccggcggc ccatcaccgc cgggacgaag gtcaacgcgg tgaacaggca tgtgatcgag
     3061 ccgatcagcg cgacttgacc gaagctgcgc agggccgggt agttgccgag ccacagcgtg
     3121 ccgaagccgg cgatgttggt cagcgcggcc atcacgacgg ccttgcccgt ctcgagcagc
     3181 ccgtcgctga cctcgccgcc gctcagcgtc agccgatgca ccaagtggat gctgtagtcg
     3241 atgccgacg
//
LOCUS       DASPGN010000184         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_850414, whole genome shotgun sequence.
ACCESSION   DASPGN010000184 DASPGN010000000
VERSION     DASPGN010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850414"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VD788_05215"
     CDS             complement(<1..894)
                     /locus_tag="VD788_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75699.1"
                     /translation="MLVCALALPATVAASTHRASSSPVLDAVEVGSTLTGEFGFPRPT
                     GLAYVPSKKLLLVASTGPGRTRFLRIGPLEESLGAFSLPKISNTSTLAFDPFRSQLTA
                     IDGKKRIAVDASELGTKQPTSERADVRNLQLKNAQGASFDPKSGTWLLLDSGAREIVR
                     VPAEDGVYRKPTRVSLKSLGDGRLRGLARNPLDGLLYVASPERRLLFAVDRSGKVAKT
                     FDIAGARIRNLRALVFAPSADRTDRPRVQSLYIADAGGGRLEGRIIELSLAPRSRLSK
                     TFAGTLAKTIHTSRLPTPAPDP"
     gene            1123..>2001
                     /locus_tag="VD788_05220"
     CDS             1123..>2001
                     /locus_tag="VD788_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75700.1"
                     /translation="MAVVAVPTAASGALSGQATPTGPAANSTSYQDSAGENPAAPDIT
                     TLTTSNNDAGVISFKIDIPNRPTLTQDLLLLIFADTDANPQTGDPESLGSDYVIQVFA
                     GEAALFRWDGADFTRRAGDPPATSLIFSYQGGVTLTISAAELGNTKRFGFAVIAVSGI
                     VIDPATGDLDFTNAVSDVAPASGAGLYAYEVRITPPSLVVRRFTPSPRIPTAGRAFTL
                     RLVAARSDTGAVVQNGRVNCVGRIGNARLRAQVQRVVAGAATCTWNIPPRAKGKTFRG
                     SVAVVFEGLRAAQSFSR"
BASE COUNT          326 a          664 c          686 g          325 t
ORIGIN      
        1 ggggtcgggc gccggtgtgg gaagccgcga ggtgtggatc gtctttgcca gcgttccggc
       61 gaacgtcttc gagagccggg agcgtggcgc cagcgacagt tcgatgatac gaccctctag
      121 cctgccaccg cccgcgtcgg cgatgtacag gctctgaact ctcgggcggt ccgttcggtc
      181 ggcgctcggc gcgaagacca gtgcccggag attccggatt cgggcgcccg cgatgtcgaa
      241 cgtcttcgcg acctttcccg accgatcgac cgcgaagagg aggcgccgct cggggctggc
      301 gacgtacagc aggccgtcga gggggttgcg tgcaagtccg cgcaacctcc cgtcgccgag
      361 gctcttgagc gacacccggg tcggttttcg atacacgcca tcctcggccg gcacgcgcac
      421 gatctcgcgg gcgccgctgt caaggaggag ccaggtaccg gatttcggat cgaacgaagc
      481 gccctgggcg ttcttcagct ggaggttcct gacgtcagcg cgctcgctgg tcggttgctt
      541 ggtcccgagc tcggaggcat cgaccgcgat gcgcttcttg ccgtcgatcg cggtcaactg
      601 cgaacgaaaa ggatcgaacg ccagtgtgga tgtgttcgag atcttcggca ggctgaacgc
      661 gcccagtgac tcctcgagcg gcccgatccg caagaatcgc gtccggccgg gaccggtcga
      721 cgcgacgagc agcagcttct tcgagggcac gtacgcaagc ccggtcgggc gagggaaccc
      781 gaactcaccg gtgagggtgg aacccacctc gaccgcatcg aggacgggac tggaggacgc
      841 acggtgcgtt gaggcggcga cggttgcggg tagcgcgagc gcgcagacga gcacgcacgc
      901 gataagacga ccgtcgcgcc tcgattcacc atagagttgg atgcgtcgca tcgacagcgt
      961 gcgcggtcag tgtacgcacc cgctcagtcg ggtcccgttg tggcgaggtt ggcttccccc
     1021 tacatttgac ggcggacatt tgacggcggc tgcgacgcgc aactaccctg acgcgcggac
     1081 gtgcgaccgc tcactcgccc agttctcctg ctcgcgatcg ccgtggcggt cgtcgcggtt
     1141 ccgaccgccg cctccggtgc gctctccggg caggcgacac cgacgggccc ggccgccaac
     1201 agcacgtcgt atcaggactc ggcgggggag aacccggccg ctcccgacat cacgaccttg
     1261 acgacgtcga acaacgacgc gggcgtcatc tcgttcaaga tcgacatccc gaatcggccg
     1321 acgctgacgc aggatctcct gctcctcatc ttcgccgaca cggatgcgaa cccgcagacc
     1381 ggagatccgg agtctctcgg ctcggactac gtgatccaag tcttcgccgg cgaagcggcg
     1441 ctcttcaggt gggacggagc cgacttcaca cggcgcgctg gagatccgcc cgcgacgtcg
     1501 ctgatcttct cgtaccaggg tggcgtgacc ctcacgatca gcgcggcgga gctcggcaac
     1561 accaagcgct tcggcttcgc cgtcatcgcg gtctcgggca tcgtcatcga cccggcgacc
     1621 ggcgatctcg acttcacgaa cgccgtcagc gacgtcgcac ctgcctcggg tgcgggactg
     1681 tatgcctacg aggtcaggat cacgccgccg tcgctggtcg tgaggagatt cacgccgtcc
     1741 ccgaggatcc cgacggccgg ccgcgcgttc acactgcggc tcgtcgcggc acgctcggac
     1801 acgggcgccg tcgtccagaa cgggcgggtc aattgcgtgg gacggatcgg taacgcgcgg
     1861 ctcagggcgc aggtacaacg cgttgtcgcc ggcgctgcga cctgcacgtg gaacattcca
     1921 ccccgggcga agggcaagac gttcagaggc tcggtagcgg tggtgttcga gggcctcaga
     1981 gcggcgcaga gcttctcgcg g
//
LOCUS       DASPGN010000185         4885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_732990, whole genome shotgun sequence.
ACCESSION   DASPGN010000185 DASPGN010000000
VERSION     DASPGN010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732990"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1494
                     /locus_tag="VD788_05225"
     CDS             73..1494
                     /locus_tag="VD788_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75701.1"
                     /translation="MITALRVATIALGLAVAILPARAQTSDASKIRELEARLVELERT
                     LETLRAESDVPTERIEELERAIAVLSHEVEDLKLGDAATVTSDRGSFGLGPAASKVYR
                     SERGVSVGGYGEMLYESFDDARQDGQPSDAIDQLDFLRAVLYFGYKFNDRILFNSEIE
                     WEHATTSNGGNGEVSVEFAYLDFTFHDLINARAGMVLVPMGFLNELHEPPIFLGARRP
                     YVEQALLPTTWRENGAGVFGENDRISYRAYVHTALAGVEGNSSGSSGFDQGGVRNGRS
                     KGSRSPADELAISGRFDWFAAPGLVVGLSAYRGDAGQGASVNTDGSGGTVDAVTTIVD
                     AHVDYRWRGLQARGVWVDTTIDDVARLNAAQAARGVLEPGESIGESQYGWYAEVGYDL
                     LAASSGTEQALIPYLRYERYDTQDEVPEAFDRDPSFDRTVVTAGVAWKPITQVVVKAD
                     YNRLENEADTGVDQINAALGFMF"
     gene            1517..2044
                     /locus_tag="VD788_05230"
     CDS             1517..2044
                     /locus_tag="VD788_05230"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HXV75702.1"
                     /translation="MLVTVWLGATLVTLPKVFLTRDAALARAFGDEAEVTRRTVFLSE
                     RQVAAGRELAGPGIELRSAMVVQYVGRLGDRLLGTAYFDTHRVRTLQETLMVVVDAEG
                     KVASVTVLTFDEPENYLPRDAWYRQFDGRGLDEQLALKRGVHGITGATLSAEATTDAV
                     RRVLAVHAVIEEARR"
     gene            2041..2535
                     /locus_tag="VD788_05235"
     CDS             2041..2535
                     /locus_tag="VD788_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75703.1"
                     /translation="MTRFERLLLGGSALLTGASGLIYAWMKYLLTTDDPYAVVNHPLQ
                     PLLLKIHVVTAPLLVFAIGLIFMQHVVRQWKSRRPSGRISGLTIGLLGVPMVLSGYLI
                     QVVTAGSLLAWLIGTHLVTGTLYLVLFGVHRWRMRAGAAQRARRERDAIGAVTEAGDR
                     EECA"
     gene            complement(2532..3284)
                     /locus_tag="VD788_05240"
     CDS             complement(2532..3284)
                     /locus_tag="VD788_05240"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXV75704.1"
                     /translation="MLVREVVAGFDGKVTFVNEDFGESALAARFGIRRYPAVFVDEVL
                     IAKPKDFGFFGEGGSQGEGRYTPWKDAASHARFKSDLERMIGFALARDTARLRAGGVD
                     PTAESPLPASLPAFSLVDLDGRPLSSDQLAGRVVIVEFWASWCPPCMSTLAWLDRLDE
                     RYGDRVALVALAVESLEDQVVALARAHGGGTRWAMATAEVAVAFGDVVAVPTMLVFDR
                     RGRAAATYHGAPPGLHEQVERLLGELLGRPGD"
     gene            complement(3306..4397)
                     /locus_tag="VD788_05245"
     CDS             complement(3306..4397)
                     /locus_tag="VD788_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75705.1"
                     /translation="MSRGRRVVVTGLAWLLGASSAMATPTESPRERIASELERLRRAA
                     GAVTSGREEWTRAKPQILEQLDEAATDLGAGRTELALVALGAAAAHLQGYLLMVADGG
                     SADEAESRFATIRDEQQSRLRADESAYRERSRTGTAASRALEQAAAARAMRYHRAAAP
                     YADATGVDAGYYYLGLARGLYEYASFVSGIDWGERPPLPALRSIDSELTALERRVTDS
                     YRPPASIEHHDDFIRVNASLKLARELDEADERFGALYQYLETLRLFAVVGADADRGLD
                     AEALALASRDSVERIEREQVDHTIAEMFLQLARSAATRSDDAAGRATARDVVDRVLPA
                     YFAVLDGKGDDLSEVDGLVTVTLVRWPYT"
     gene            complement(4471..>4885)
                     /locus_tag="VD788_05250"
     CDS             complement(4471..>4885)
                     /locus_tag="VD788_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75706.1"
                     /translation="PPAGAAPDRLAPGEAPTPPAEPATGASGLAVELSKIGWGGSMLL
                     PDLRMEGSVHGAKIARAEVRVVDARGNEIQRDRLQDVERFLARVADRDEPMSIRFTYE
                     VDGKRMVLAKYPVSVHVTAYDTDGGTSEKVVEVDR"
BASE COUNT          737 a         1712 c         1678 g          758 t
ORIGIN      
        1 gtcgcctcta atgtccgccg ttggttctgt ttgttctttt acgcgagtaa ttcgcaacaa
       61 gaagggaatt cgatgatcac cgccctccgc gtcgccacga tcgcgctcgg tttggccgtc
      121 gccatcctgc cggcacgagc ccagacgagc gacgcatcga agatccgcga gctcgaagcg
      181 cgcctggtcg agctggaacg gacgctcgag acgctgcgag ccgagtcgga cgtcccgacc
      241 gaacggatcg aggagctcga acgagcgatc gcggtcctgt cgcacgaggt cgaggatctc
      301 aagctcggcg atgccgcgac ggtcacctcc gaccgcggga gcttcggact cgggccggcg
      361 gcgtccaagg tctaccgcag cgagcgcggc gtgtcggtcg gcggctacgg cgagatgttg
      421 tacgagagct tcgatgacgc gcgtcaggac ggccagccat cggacgcgat cgaccagctc
      481 gacttcctac gtgcggtcct ctacttcggc tacaagttca acgaccgcat cctgttcaac
      541 tccgagatcg aatgggaaca cgcgacgacg tcaaacggcg gcaatggcga ggtctcggtc
      601 gagttcgcct acctcgattt caccttccac gacctgatca acgcccgggc cggcatggtg
      661 ctggtgccga tgggattcct caacgagctg cacgagccgc cgatcttcct cggcgcccgc
      721 cgtccgtacg tcgagcaggc actgctgccg acgacctggc gcgagaacgg cgcgggggtg
      781 ttcggcgaga acgaccgcat ctcctatcgc gcctacgtgc acaccgcgct cgccggtgtc
      841 gagggcaatt cgagcggctc gagcggcttc gatcaaggtg gcgtgcgcaa tggccgcagc
      901 aaaggctcga ggtcgccggc ggacgagctg gcgatcagcg gccggttcga ttggttcgcg
      961 gcgccgggtc tggtcgtcgg tctgtcggcc taccgcggcg acgccggtca gggggcgtcg
     1021 gtcaataccg acggcagcgg cgggacggtc gacgccgtca cgacgatcgt cgacgcacac
     1081 gtcgactatc gctggcgggg tctccaggcg cgtggcgttt gggtcgacac tacgatcgac
     1141 gacgtggctc ggttgaacgc ggcccaggcg gcgcgcgggg tgctcgagcc gggcgagtcg
     1201 atcggcgaga gtcagtacgg ctggtacgcc gaagtcggct acgatctgct cgccgcatcg
     1261 agcggaaccg agcaggcgct gatcccgtac ctccgctacg aacggtacga cacgcaagac
     1321 gaagtacctg aggccttcga ccgcgacccg tctttcgacc gcaccgtggt gacggcgggc
     1381 gtcgcctgga agccgatcac ccaggtggtg gtcaaggccg attacaaccg cctcgagaac
     1441 gaggccgata cgggagtcga tcagatcaat gccgcgctcg gcttcatgtt ctagcgccgc
     1501 gtggcgctgg acgagcgtgc tcgtcacggt ctggctcggt gcgacgctcg tcacgttgcc
     1561 caaggtgttc ctgacgcgcg acgccgcgct ggcacgggcg ttcggcgacg aggcggaggt
     1621 cacgcggcgg acggttttcc tgtcggagcg gcaggtcgcc gcggggcgcg agctggcggg
     1681 gccgggaatc gagcttcgca gtgcgatggt cgtgcagtac gtcggacggc tcggcgaccg
     1741 gctgctcggc accgcctact tcgatacgca tcgcgtgagg acgctgcagg agacgttgat
     1801 ggtggtcgtc gacgcggaag gcaaggtcgc gtcggtcacg gtcctgacct tcgacgagcc
     1861 ggagaactac ctcccgcgcg acgcgtggta ccgccagttc gacggccgcg ggctcgacga
     1921 gcagctggca ctgaagcgag gcgttcacgg catcacgggg gcgacgctca gcgccgaggc
     1981 gacgaccgac gcggtccgtc gcgtgctggc ggttcatgcg gtgatcgagg aagcccgccg
     2041 atgacccgtt tcgagcggtt gttgctgggg gggtcggcgc tgttgaccgg cgcgagcggt
     2101 ctgatctacg cctggatgaa gtacctgttg acgaccgacg atccgtacgc ggtcgtcaac
     2161 cacccgctgc aaccgctgct gctgaagatc cacgtcgtga ctgcaccgct gctggtcttc
     2221 gcgatcgggt tgatcttcat gcagcacgtc gtgcggcagt ggaagtcccg ccgtccgtcg
     2281 ggccggatca gcggcctgac gatcggcctg ctcggcgtgc cgatggttct ctccggctat
     2341 ctgatccagg tcgtcacggc cggctcgctg ctggcctggt tgatcgggac gcacctcgtc
     2401 accgggacgc tgtatctcgt cctcttcggc gtccaccgct ggcgcatgcg cgcgggtgcg
     2461 gcgcagcgcg cccgtcgcga gcgagatgcg atcggcgcgg tcaccgaagc cggcgaccgg
     2521 gaggagtgcg cctagtcccc cggcctgccg agcaactcgc cgagcagccg ctcgacctgc
     2581 tcgtgcaggc cgggaggcgc tccgtggtag gtcgccgccg ccctcccccg ccgatcgaag
     2641 acgagcatcg tcggcaccgc gaccacgtcg ccgaaggcga cggcgacctc ggcggtcgcc
     2701 atcgcccagc gcgtgccgcc cccgtgggcg cgggccagcg cgacgacctg gtcctcgagc
     2761 gactcgacgg cgagcgcgac caacgccacg cgatcgccgt accgctcgtc gagacgatcg
     2821 agccaggcca gcgtggacat gcagggcggg caccacgatg cccagaactc gacgatcacg
     2881 acgcggcccg cgagctggtc ggacgacagc gggcgaccgt cgagatcgac gagcgaaaag
     2941 gccggcagcg acgcggggag cggagactcc gcagtcgggt cgaccccgcc ggctcgcagg
     3001 cgcgccgtat ctcgcgccag cgcgaacccg atcatccgct cgaggtcgct cttgaaccgc
     3061 gcgtgacttg ccgcgtcctt ccacggtgtg tagcggccct cgccctggct gcctccctca
     3121 ccgaagaagc cgaagtcctt cggcttggcg atcagcacct cgtcgacgaa caccgccgga
     3181 tagcgccgga tgccgaagcg ggcggccagc gcggactcgc cgaagtcttc gttgacgaac
     3241 gtgactttcc cgtcgaaccc cgccaccacc tcccgtacca gcaaacttgc cgggtccgag
     3301 aggtttcagg tgtacggcca gcggacgagg gtcacggtca ccagcccgtc gacctccgac
     3361 aggtcgtcgc cctttccgtc gagcacggcg aaataggccg gcagcacccg gtcgacgacg
     3421 tcgcgcgcgg tggcgcgacc ggccgcatcg tcggaccgcg tggccgcgct acgagccagc
     3481 tgcaagaaca tctccgcgat cgtgtgatcg acctgttccc gctcgatccg ctcgacgctg
     3541 tcgcggctcg ccagagccag cgcctcggcg tcgagcccgc ggtccgcatc ggcaccgacg
     3601 acggcgaaca agcggagcgt ctcgaggtac tggtacaacg cgccgaagcg ctcgtccgcc
     3661 tcgtcgagct cgcgagccag cttgagcgac gcgttgacgc ggatgaaatc atcgtggtgc
     3721 tcgatcgacg ccggcggccg gtaggagtcc gtcacgcgtc gctcgagagc ggtcaattcg
     3781 gaatcgatcg agcgcagcgc gggcaacggc gggcgctcgc cccaatcgat cccgctgacg
     3841 aacgaggcgt attcgtacag tccgcgcgcc agcccgaggt agtagtagcc ggcgtcgacg
     3901 ccggtggcgt cggcgtaagg cgcggccgcg cgatggtacc gcatcgccct ggcggcggcg
     3961 gcctgctcca gcgcgcgcga ggcggccgtt ccggtccggc tccgctcgcg ataggcgctc
     4021 tcgtccgccc gcagccggct ctgctgctcg tcgcggatcg tcgcgaaccg gctttccgcc
     4081 tcgtccgccg aaccgccgtc ggccaccatc agcaggtagc cctggagatg ggccgctgcc
     4141 gcgcccaggg cgacgagggc gagctcggtg cggcccgccc cgaggtcggt cgccgcctcg
     4201 tccagctgct cgaggatctg cggcttggcg cgcgtccatt cttcgcgccc gctcgtgacg
     4261 gccccggccg cccgccgcag ccgctcgagc tcgctcgcga ttcgctcgcg cggggattct
     4321 gtcggggtcg ccatcgccga tgacgccccg agcagccagg ccaagccggt gaccaccacg
     4381 cgccgacccc gcgacatccc cgtccctccg ccaccactcg tcagtggggc atacgggagc
     4441 gcagggcgcg ggttgcagcc gtccgccgcc tcagcgatcg acctcgacga ccttctcgct
     4501 cgtgccgccg tccgtgtcgt acgccgtgac gtgcaccgac accggatact tggccagtac
     4561 cattctcttc ccgtcgacct cgtaggtgaa ccgtatcgac atcggctcgt cgcggtcggc
     4621 cacccgcgcc aggaagcgct cgacgtcctg caaccggtcg cgctggatct cgttgccgcg
     4681 ggcatcgacc acgcgtacct ccgcgcgcgc gatcttcgct ccgtggaccg agccctccat
     4741 tctcagatcc ggcagcagca tcgatccgcc ccagccgatc ttgctcagct cgaccgcgag
     4801 cccactcgcg cccgtcgccg gctcggcagg gggcgtcgga gcctcgcccg gggcgagtcg
     4861 gtccggcgcc gctccggcgg ggggc
//
LOCUS       DASPGN010000186         4974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_265462, whole genome shotgun sequence.
ACCESSION   DASPGN010000186 DASPGN010000000
VERSION     DASPGN010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265462"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VD788_05255"
     CDS             complement(<1..802)
                     /locus_tag="VD788_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXV75707.1"
                     /translation="MKHHFVVVAHDDRVREALAGELRSRGYSVTRAASARQTEQIVDT
                     VAVDVVIVESHLPDMPPEELRARLIRARPACRVIVLSDFRRVRNTPEQLAFGDNAFLL
                     RSSEVLELVEAPFRAKPNGDGASFSERGNAALIQALDVVVGLVELDDHHFGGFSHQAM
                     GLAREVAVQLSSDQQLADEVAIATLVRDLGRAGVSRDVLDEKGWYSSDQRERMKAHVE
                     GSFKLFEQIDFPWKVLPIIRHHHERYDGNGYPDGLKGPEIPLGARIIAV"
     gene            complement(899..1375)
                     /locus_tag="VD788_05260"
     CDS             complement(899..1375)
                     /locus_tag="VD788_05260"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXV75708.1"
                     /translation="MSIKNRLTEDAKQALKAGDKTRVACLRMLRAKILEREVALRPKK
                     GTTYELDDDEALQVLTSYAKQRQESIDSYHQGGREDLVAREQAELEIVRHYLPEPLSD
                     ERIRHIVAESVAELGATSPREMGAVIKRVMARTRGAADGRLVSRLVREALEDGGTP"
     gene            complement(1391..2839)
                     /locus_tag="VD788_05265"
     CDS             complement(1391..2839)
                     /locus_tag="VD788_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HXV75709.1"
                     /translation="MNYRGVFFLLGRLLIALALALLAPAAVAWFFDDGVWTAFVGPAL
                     LAAVAGIVLQRWNRQEADFPFSRREAFLLVTAAWVTASVIGALPYLVLKGPQFAVDAL
                     FESASGFTTTGASVLADVENEPHGLLLWRSLTQWLGGMGIIVLGIAILPKLAIGGMEL
                     LGAEAPGPMQEKLTPRIAQTAKYLWGIYALLTGAEVAVLIVLGLPPLEAINHALTTMA
                     TGGFSTRNASVASFASPAIELAVTGFMFLAGVNFALHFWWLRGRPRRLFGEPEFRFYC
                     GVLLGASLLLALDLVLVDGHRALDAARLAVFQSTSVVTTTGYATANFDTWPSFSKVLL
                     FLLMFVGGCAGSTGGSVKVVRIMIVAKKLVVDLKRLVQPHAVLPVRIGRRAVPEEIVA
                     SVTTFFILFLTLFAAGGLLLGLMGLDMVSAFSASATCLGNIGPGLGAVGPAATYGPLP
                     DVAKLLLVGLMIVGRLELYTVLVLVFMWRRTV"
     gene            complement(2836..4167)
                     /gene="trkA"
                     /locus_tag="VD788_05270"
     CDS             complement(2836..4167)
                     /gene="trkA"
                     /locus_tag="VD788_05270"
                     /inference="COORDINATES: protein motif:HMM:NF007039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HXV75710.1"
                     /translation="MKVFIVGAGEVGIHIAQSLVREGHDLVLIERDAKKVSQLQSKID
                     VLAVCGDGCNPRTLRQHGAGDADLFFAVSDKDPVNLLAALTARELGARRAVVRVADPD
                     LGRNPLVQKDKELLLLYPERLVAQEIFSLTRVPGAGKARFFREGKLVLLQARPSIMAP
                     IYGRPVKDLVGPKNWILTGIHRATGTVIPRGDTVLRPGDLIFSVGPTETMHDYLASIG
                     VESNPTKKVVIAGAGQVGASLARLLVKEKIRVVVVQRGASKAFDLAAELPEVLVLRGD
                     ATEPEMLRDAGIQDADYFVAATQRDEANLLTSLLARELGARAVVALYNQPEFLSVMQA
                     VRIDIPLSPRLMIAGHILRMVHRQEIISMDLVAGGNAEVLELVVPARARVSKRPLNRL
                     NFPRDAIVGAVIRQNESFVPTGEFQFAEGDRALVFTLTETLPEVERMFRGR"
     gene            complement(4230..>4974)
                     /locus_tag="VD788_05275"
     CDS             complement(4230..>4974)
                     /locus_tag="VD788_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HXV75711.1"
                     /translation="ETAWRAIGEASLPPGSARFVLGVIGGVGGSVTLLSYAYWIDEKG
                     WSGPDRLTTVRVDLRVAYLLTGIFAVAVMSLAAVTLHPAGTTIQGNSGALVMADMLVD
                     VVGPLGRWIFLVGFWGAVATSMLGVWQGIPYLFCDFVALMRGLAPPERRRVIEARSPW
                     YRGYVVFLALPPLGLLAFGRPVALVVVYSVLGALFMPFLAATLLYMNSRPAWVGSRLR
                     NGVTTTLLLVLCLVLFTLIAAVDVSRQFD"
BASE COUNT          883 a         1747 c         1637 g          707 t
ORIGIN      
        1 cgacggcgat gatgcgggcg ccgagcggaa tctcggggcc cttcagtccg tcgggatagc
       61 cgttgccgtc gtagcgttcg tgatggtgcc tgatgatcgg cagtactttc cacgggaagt
      121 cgatctgctc gaacagcttg aagctgccct cgacgtgcgc tttcatccgt tcgcgctggt
      181 ccgaggagta ccagcccttt tcgtcgagca cgtcgcgcga gacgcccgcc cggccgagat
      241 cgcggacgag cgtggcgatc gccacctcgt cggccaactg ctggtcggac gaaagctgca
      301 cggccacctc gcgggcgagg cccatcgcct ggtgggagaa gccgccgaag tggtgatcgt
      361 ccagctcgac caggccgacg acgacatcga gcgcctggat cagcgccgcg tttccacgct
      421 cggagaacga cgctccgtcg ccgttgggct tggcccggaa cggcgcctcg accagctcga
      481 gcacctcgct cgagcggagc aggaaggcgt tgtcgccgaa ggcgagctgc tcgggggtat
      541 tgcggacgcg gcggaagtcg gacagcacga tcacgcggca ggcggggcgg gcgcggatca
      601 gacgcgcgcg cagctcctcg gggggcatgt cgggcaggtg cgactcgacg atgacgacgt
      661 cgaccgcgac ggtgtcgacg atctgctcgg tctggcgcgc gctcgccgcc cgcgtcaccg
      721 agtagccgcg cgagcgcagt tccccggcga gtgcctcgcg gacccgatcg tcgtgcgcga
      781 cgacgacgaa atggtgcttc acggcgcctc cgacggccga ccttccctac acgacccggc
      841 gtcggcgaat ataggttgtg cggccgacaa gtcaaaaacg gccggaaggc gggggcctct
      901 acggcgtccc tccgtcctcg agcgcttcgc gcacgagacg gctgacgagc ctaccgtccg
      961 ccgcgccgcg cgtcctcgcc atcacccgct tgatcacggc tcccatctcg cgcggcgagg
     1021 ttgcgccgag ctcggccacc gactcggcga cgatgtgtcg gatccgttcg tcggacagcg
     1081 gctcgggcag gtagtgccgg acgatctcga gctcggcctg ctcgcgcgcg acgagatcct
     1141 cgcgcccgcc ctggtggtag ctgtcgatcg attcctgacg ctgcttggcg tagctcgtca
     1201 gcacctgcaa cgcttcgtcg tcgtcgagct cgtaggttgt ccccttcttc ggtcggagcg
     1261 ccacctcgcg ttcgaggatc ttcgcccgca gcatgcgcag gcacgccacc cgggtcttgt
     1321 cgccggcctt gagcgcttgc ttggcgtcct cggtcaatcg gttcttgatc gacatctcgt
     1381 cctctccggt tcaaaccgtt cggcgccaca tgaacacgag caccagtacc gtgtagagct
     1441 ccagacgtcc gacgatcatc agcccgacca gcagcagctt ggccacgtcg ggcagcggcc
     1501 cgtaggtcgc tgccgggccg accgcgccca gtcccgggcc gatattgccg agacaggtcg
     1561 ccgacgccga gaacgcgctc accatatcca accccatcaa cccgagcaac aggccgccgg
     1621 cggcgaacag cgtcaggaac aggatgaaga acgtcgtcac cgacgccacg atctcctccg
     1681 gcaccgcccg ccggccgatg cgcaccggta gcacggcgtg cggctgaacc agccgtttca
     1741 gatcgaccac cagcttcttg gccacgatca tgattcggac gaccttgacc gagccgccag
     1801 tcgatccggc acagccgccg acgaacatca acaggaacag cagcaccttg gagaacgacg
     1861 gccaagtgtc gaaattcgcc gtcgcgtatc cggtggtggt gacgaccgac gtcgactgga
     1921 aaaccgcgag ccgtgccgcg tccagcgcac ggtggccgtc gaccagcacc aggtcgagcg
     1981 ccaagagcaa gctcgcgccg agcagcacgc cgcagtagaa gcggaactcg ggctcgccga
     2041 acagccgccg cggccgaccg cgcagccacc agaagtgcag cgcgaagttc acgccggcca
     2101 agaacatgaa cccggtgacg gccagctcga tcgccgggct cgcgaaggag gcgacgctgg
     2161 cgttgcgcgt cgagaacccg ccggtggcca tcgtggtgag ggcgtggttg atcgcctcga
     2221 gcgggggtag accgagcacg atcaagaccg cgacctccgc gccggtcaac agcgcgtaga
     2281 tgccccacag gtacttcgcc gtctgcgcga tgcgcggagt cagcttttcc tgcatcgggc
     2341 ccggcgcctc ggcaccgagc agttccattc cgccgatcgc cagcttcggc aggatggcga
     2401 tccccagcac gatgatgccc atcccgccca accactgggt cagcgagcgc cacagcaaca
     2461 agccgtgcgg ctcgttctcg acgtcggcca gcaccgacgc tccggtggtg gtgaagcccg
     2521 acgccgactc gaacagcgcg tcgacggcga actgcggacc cttgagaacg agatacggca
     2581 gggcgccgat gaccgaggcg gtgacccacg ccgcggtgac gagcaggaac gcctcgcgcc
     2641 gcgagaacgg gaagtcggcc tcttgccggt tccaccgttg caggacgata ccggcgacgg
     2701 cggcgagcag cgccgggccg acgaacgccg tccacacgcc gtcgtcgaag aaccaggcga
     2761 ccgcggccgg tgcgagcagc gccagcgcga gagcgatcag cagacgcccg agcagaaaga
     2821 acacgccgcg gtagttcatc gtccgcggaa catccgctcc acctccggca acgtctcggt
     2881 cagcgtgaag accagcgccc gatcaccctc ggcgaactgg aactcgccgg tcgggacgaa
     2941 gctctcgttc tggcgtatca ccgcgccgac gatcgcgtcg cgcgggaagt tgagacggtt
     3001 caacggccgc ttcgacacac gcgcccgggc gggtacgacc agctcgagca cctccgcatt
     3061 gccacccgcg acgaggtcca tgctgatgat ctcctggcgg tgcaccatgc gcaggatgtg
     3121 gcccgcgatc atcaatcgcg gcgacaacgg gatgtcgatg cgcacggcct gcatcacgct
     3181 gagaaactcc ggctggttgt agagcgccac gaccgcccgc gcgccgagct cgcgggcgag
     3241 cagcgacgtc aacaaattcg cctcgtcgcg ctgggtcgcc gcgacgaagt agtcggcgtc
     3301 ctggatgccg gcgtcgcgca gcatctcggg ctcggtggcg tcaccccgca gcacgagcac
     3361 ctcgggcaat tcggcggcga ggtcgaacgc cttcgacgcg ccgcgctgga cgaccacgac
     3421 ccggatcttc tctttgacca acagccgagc gagcgaggcg ccgacctgcc cggcgccggc
     3481 gatgacgacc ttcttggtcg ggttcgattc gaccccgatc gacgccaggt agtcgtgcat
     3541 cgtctcggtc gggccgacgg agaagatcag gtctcccggg cgaagcaccg tgtcgccgcg
     3601 cgggatgacc gtaccggtcg cacggtggat cccggtgaga atccagttct tcggtccgac
     3661 cagatccttc accggcctcc cgtagatcgg cgccatgatc gacggccggg cctgcagcag
     3721 gaccagcttc ccctcgcgaa agaaccgcgc cttgcccgct cccggcacgc gggtcaggct
     3781 gaagatctcc tgcgcgacga gccgttcggg gtagagcaac agcagctctt tgtccttctg
     3841 aaccaacgga ttgcgaccca gatcgggatc ggccacgcgc accaccgcgc gccgcgcacc
     3901 caactcccgc gcggtcagtg cagccagcag attgaccgga tccttgtcgg atacggcgaa
     3961 gaacaggtcg gcgtcgccgg cgccgtgctg tctgagcgtt cgcgggttgc agccgtcgcc
     4021 gcaaaccgcg agcacgtcga tcttgctctg gagctggctg accttcttgg cgtcgcgttc
     4081 gatcaggacg aggtcgtgcc cctcgcgaac gagcgactgg gcgatgtgga tcccgacttc
     4141 gcccgcaccg acgatgaaga ccttcatagg ccgccgatgc taccaccacg accgggcgcg
     4201 cggccggtcg gcctgcaccg gctctcgggt cagtcgaact ggcgcgagac gtcgacggcg
     4261 gcgatcagcg taaacagcac cagacacagc accagcagca acgtcgtcgt cacgccgttc
     4321 cgcaagcgtg agccgaccca ggccggtcgc gagttcatgt agagcagcgt ggcggccaag
     4381 aacggcatga acagcgctcc cagcacggaa tagacgacga ccagcgccac cggccggccg
     4441 aaggccagca agccgagcgg cggcagcgcc aggaacacga cgtaaccgcg gtaccacggc
     4501 gagcgggctt cgatcacgcg cctccgctcc ggcggcgcga gaccccgcat cagcgcgacg
     4561 aaatcacaga acaggtacgg gatcccttgc cagacgccga gcatcgacgt ggcgaccgct
     4621 ccccagaatc cgacgaggaa gatccagcga ccgagcggac cgacgacgtc gaccagcatg
     4681 tcggccatca ccagcgcccc gctgttgcct tggatcgtcg tccccgcggg gtgaagcgtg
     4741 acggcggcga gcgacatgac ggcgacggcg aagatgccgg tcagcaggta ggccaccctg
     4801 agatcgacac gcaccgtggt cagtcggtcg gggccggacc agcccttctc gtcgatccag
     4861 taggcgtagg acagcagggt gacgctgcct ccgaccccgc cgatcacgcc gaggacgaac
     4921 cgcgcgctcc cgggcgggag gctcgcctcg ccgatcgcgc gccaggccgt ctcc
//
LOCUS       DASPGN010000187        17446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1669981, whole genome shotgun sequence.
ACCESSION   DASPGN010000187 DASPGN010000000
VERSION     DASPGN010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 17446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17446
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1669981"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VD788_05280"
     CDS             complement(<1..336)
                     /locus_tag="VD788_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75712.1"
                     /translation="MVDRLLAAGLALAGLTSAAPVAALDRQSPRNANYTIEVRLDPVT
                     RMLAGRQRLTWRNIRTRPTSELWFHLYWNAWRNDRSSWMLERRLSSPRDDRPIRADDW
                     GFVEVDSIRM"
     gene            complement(410..1348)
                     /locus_tag="VD788_05285"
     CDS             complement(410..1348)
                     /locus_tag="VD788_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75713.1"
                     /translation="MPTTVIAVLLALASGGAPGEVDRPAVSQGFSLLGKPLFPPPLPE
                     QVEIERRRQLKEAHLRWEQNPGDVEAAIWVGRRTAYLGLYQDAIEVYDRAIERFPDDP
                     RLYRHRGHRYITVRKFDLAIADLERAVKLIQGTEDRVEPDGLPNARNQPTSTLHGNIW
                     YHLGLAYYLEGDFEQALRCYRECLALATNPDMLCATSHWMYMTLRRMGRTEQARALLE
                     PIDAALDVIENHAYHELLLMYKGQRDAGELLERAAAGDGPEFAATAYGVANWDLYRGH
                     QRRAYERFEALLADDQWAAFGYIAAEAELASRRGDR"
     gene            complement(1369..2496)
                     /locus_tag="VD788_05290"
     CDS             complement(1369..2496)
                     /locus_tag="VD788_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnH family di-heme enzyme"
                     /protein_id="HXV75714.1"
                     /translation="MRRAGSQPLVVVVLVVAASVADGAEFAWRLPRGFPVPLVPAHNP
                     MSEAKVELGRRLFHDARLSPNGTTACATCHRPELAFTDGKARAVGATGETHPRGAMSL
                     ANVAYNASLNWADPDVRTLERQAHGPMFNDAPIEMGIAGNEQVILARLRLDARYPALF
                     AAAFGAGETITLDNVARAIASFERTLISGDSPYDRWVYHGETDGIDEATRRGMRLFFS
                     DRLRCAECHGGFNFGGPVVFEGSADVAPTFHNTGLYDVDGRGAYPADNPGLYAATGDR
                     RDMGRFKAPTLRNVAVTAPYMHDGSIGTLAGVIEFYAAGGREVDAGPDAGDGRANQNK
                     SELIGGFALTTAERDDLLRFLDSLTDRRFLADPAHRRPAGD"
     gene            complement(2480..3145)
                     /locus_tag="VD788_05295"
     CDS             complement(2480..3145)
                     /locus_tag="VD788_05295"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75715.1"
                     /translation="MKARGFRMVATVIIAALAALAVHASGGTAQPEPTTPELTPEQLA
                     DQFYNEGLVYRDKAWKFEEELAAAAEADRAKLEKRIEKNYTRAIASFEDALRNNPNLY
                     QAASSLGYALRKTGEYDDALAAYDHALSIQPNYAEAIEYRAEAYLGLNRIDEAKESYL
                     TLFGSDRPRADELLAAMQRWLAAPPQSVDGATVESFRAWVDERAEIAGNTAPVSQLGE
                     KSW"
     gene            3252..3893
                     /locus_tag="VD788_05300"
     CDS             3252..3893
                     /locus_tag="VD788_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75716.1"
                     /translation="MNIRRLTRIVGTAWLAGAAPWAGAGEPADGREIARVASAEQLCI
                     RWLETRSEIEIEVYPQIVSIARRVIAEAFSNDLITPGATTTADVAWYIRHAEGVIRRG
                     DVLHLCTDTQEMGYVLELDEQDAPPGSSQALAAGNRWQDLLTEPFRAGRTGNEILASV
                     YTHPLGFLGHGPGPTIGMWDDQWVQIKLEQSALFDGERVRYLAGRQTRWHRVR"
     gene            3875..5203
                     /locus_tag="VD788_05305"
     CDS             3875..5203
                     /locus_tag="VD788_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HXV75717.1"
                     /translation="MASRTMSGWSRALQLADRTPATRNRYVDFLRALSIGAVVSGHWL
                     IAAPWMRDGQIRLDHMLGMQPWTQWLTWLFQVMPIFFMVGGYSNAASWTAARRAGTGY
                     GVWTRARLRRLIGPVAPLVAFWAVAGAIGYRAGVPAEMIRIASQAALVPVWFLAIYVV
                     IVLLVPWTHGAWTRFGLASVLAPAAAAVAVDVLRFRMGWTDLAWVNYLFVWAAVHQIG
                     YGWRDGRLGHAGWRLIGMLCGVAALAGLVALAGYPHSMVGVPGEPVSNTLPPSVAMLA
                     LGLAQAGLLLALEQPARRWLSGRRPWAATILVNGTIMTVYLWHMTAAILLVGLLRLFD
                     GFGLHVAPGSTTWWITRPVWVAVLSVALSPFLLVFARFERAGDGAPARHLPGWRAIVG
                     SALFCLGLALIALDGVGGGGPLGIRLWVVLLPLVGAVVAGALPAARARVG"
     gene            complement(5174..5773)
                     /gene="rfbC"
                     /locus_tag="VD788_05310"
     CDS             complement(5174..5773)
                     /gene="rfbC"
                     /locus_tag="VD788_05310"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HXV75718.1"
                     /translation="MRVVIESTPFDGVAVLQPHAHFDERGWFMESYRIDHFAELGLTE
                     PFVQDNHSRSSKGVLRGLHFQWDPPMGKLMRVTVGRAFVVAVDIRYDSPTLGRWYGRE
                     ATADNRLQVWAPAGFARGFCALSEIVEVQYKCTGTYNAPCESGIRWDDPQVGIDWPID
                     GPLLSDKDANAQTLAQWLGRPESRAFRFRPSADSRTRGR"
     gene            complement(5770..6987)
                     /gene="lhgO"
                     /locus_tag="VD788_05315"
     CDS             complement(5770..6987)
                     /gene="lhgO"
                     /locus_tag="VD788_05315"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HXV75719.1"
                     /translation="MDAPDTEIAVIGAGIVGLAAARSIASRTRSSPTVVEAENRVATH
                     QTGHNSGVVHSGLYYRPGSSKALYCIDGREAIRRFCERNGIPHECCGKVVVATSREQL
                     PALDELERRGTANGLQGLERLDPAGLERVEPHVRGVAALRVPQTGIVDFTRVARAMAA
                     ELSTRGGSLLTGWRVRRVERLSDRFRLSSDGASLTCRHLVNCAGLQSDRVARMCGVRP
                     GLRIVPFRGEYYRLRPGRERLIRHLVYPVPDPRFPFLGVHFTRMIDGGVTAGPNAVLA
                     LDRNGYSRGSFSLRDALSIASYGGFWRLAARHWRAGLDEFRRSSSRPAFVGALRRLVP
                     QLSPDDVEPHTAGVRAQAVAPDGRLLDDFHIIERERMLHVLNAPSPAATASLSIGEAL
                     AERAARAFGLEHR"
     gene            7377..9431
                     /locus_tag="VD788_05320"
     CDS             7377..9431
                     /locus_tag="VD788_05320"
                     /inference="COORDINATES: protein motif:HMM:NF019902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75720.1"
                     /translation="MPTGDQIIAKAREALDLETYRQQHWTGSFGDYLELIKRNPRITR
                     TSFERIYDMVLASGTREYIDNKKKIIHFNFFDDVASAGQDAIYGLDIALMKLVNIFKS
                     AAKRYGTERRVILLHGPVGSSKSTIARLLKRGLEAYSRRPEGALYTFGWIRDLDRPDE
                     VTPCPMHEEPLHLVPIDLRTMIEKSLNEDHTEGRYTVRIEGELCPFCRQEFRELSERY
                     GGDWTKVIRHVQVRRLLLSAQDRIGIGTFQPKDEKNQDSTELTGDINYRKIAEYGSDS
                     DPRAFNFDGEFNIANRGMIEFIEVLKLDVAFLYELLGASQEHQIKPKKFPQTFIDEVI
                     IGHTNEPEYRRLQNNEYMEALRDRTVKVDIPYITKLNEEIKIYEKDYNKAKIRGKHIA
                     PHTIEMVAMWAVLTRLEDPKKHNLTLMQKLKLYNGKSLPGFTEDNVKELRKESMREGM
                     DGISPRYIQDKISNSLVSDVSERCINPFIVLNELESGLKHHSLITSEEQRQRYRELLD
                     EVRREYEDVVKNEVQRAIAADESAISRLCGNYVDNVKAYTQKERVQNPYTGQDEEPDE
                     RLMRSIEEKIDIPESRKDDFRREIMNYIGALALEGKRFDYRTNERLHKALELKLFEDQ
                     KDSIKLTSLVSSVVDKETQEKIDIVKNRLVRDYNYCDSCASDVLHFVASIFARGDVKE
                     RG"
     gene            9444..10514
                     /locus_tag="VD788_05325"
     CDS             9444..10514
                     /locus_tag="VD788_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HXV75721.1"
                     /translation="MSRIQSDVNRFKQIVRGRVRDELRKHLGQQEMFGRQGKNIVSIP
                     IPHLELPHFAHDPGAGKGVGQGDGDGDGRKAGNEAGQHLLEAEFSVEELAHMLGEALE
                     LPRIRPKGRDELDTNAGRYTGVSTVGPESLRHYKRSYRNALKRLIATGAYDRGRPSIV
                     LERVDKRYRTWKPRQQPTANAVIVYVMDVSGSMGADQKELVRIASFWLDAWISSHYHG
                     VRSVYVVHDAAAKEVDRDTFFRIRESGGTVISSAYELVVRILAERFPAEDWNLYLFHF
                     SDGENYSRQDTEKCMQLLDKRLLPYLNLFGYGQVESYGTSGDFFEAVERHYPNDEKVT
                     MAHIPDRDAIVGCIKTFLGKGR"
     gene            10511..12031
                     /locus_tag="VD788_05330"
     CDS             10511..12031
                     /locus_tag="VD788_05330"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HXV75722.1"
                     /translation="MMKQNPLPLELALAQERIEKIAADFGLTFFRTVFEMVDYRQMNA
                     LAAYGGFPTRYPHYRFGMEYNQLSKSYEYGLHKIYEMVINNDPTYAYLLEGNTMLDQK
                     LVMAHVYGHADFFRNNMYFSQTNRKMIDAMANHATRIRRYQERYGVERVERFIDACLS
                     VEDLIDPASIFRRTSPPRELSAGLEEEETMVPGRMPAKSYMDRYVNPPEYVRQQREKL
                     AKRKEETRKFPRHPERDVLGFMMEHAPLDNWERDVVGIIREEAYYFLPQRQTKVMNEG
                     WASYWHSRIMTGKVLTDAEVIDYADHHSGTVATQPGRLNPYKLGLELFRDIERRWDMG
                     QFGPAWEACESMAERNNWDRKTGAGRDKIFEVRRIHSDITFLDEFLTPEFCDAQRLFV
                     SKNKDGKGQKAVVASREFKEVKQAMLFQFSNGGRPVIELVDANYANRSELLLRHRHVG
                     VDLRWDWAADVLAQLNRIWRRPVRLETARDGKSVRLGHDGQNVTEEVFDKKKAAGS"
     gene            12146..13348
                     /locus_tag="VD788_05335"
     CDS             12146..13348
                     /locus_tag="VD788_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV75723.1"
                     /translation="MAETCDKAFGRYLRTLRERRALTLDAVVSLSQTFPETIHKGYLS
                     RCENGFQKPAFSKIIALSRIYEVPADVLVERMELDMELDRIGGPDTAGLGFGQMTIRG
                     RESLQRGYRWPAYAFLRDAVVTARTEPPQLSFRDVEEQYSCAVMSCATAAGALGRHRF
                     AIHEFKHLKKQIRLGPRLLTLVLERISSCYLALGDISAAEHFSDRAIDASEQAGGEEY
                     RGHALYARARVALWSQDRRKAVRLFEQAYEEHKSRDRREDCALALNSLAQCYFDLRRR
                     GAARRSCRAALKIADSVGHLRTKAFSLLLLGELDELAEKPNLALRRWRAAVQIGKELK
                     DRELRFKAEFLIFRRAIRDENDAVARAIHRRLHRLAPWIPPTTQELSEFRNLSHTVTL
                     AGVSRSQR"
     gene            13829..15169
                     /gene="trmFO"
                     /locus_tag="VD788_05340"
     CDS             13829..15169
                     /gene="trmFO"
                     /locus_tag="VD788_05340"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HXV75724.1"
                     /translation="MAERVTVVGGGLAGSEAAWQIAKRGVPVRLFEMRPVRSTAVHRT
                     DRLAELVCSNSLKSLELSTAHGLLKQEMTRLGSMIVESAMANRVPAGGALAVDRERFA
                     EAVTARLSAEPLVELVREEVREIPPDGIVILAVGPLVSDSLAASIANFTGQDYLYFFD
                     AIAPVIEADSIDRTIVYPLSRYGKGDGHDYLNCPLDEAQYHAFVRELVSGEKAPLHDV
                     DATPFFEGCLPIEEMARRGPDTLAFGPMKPVGLTDPRTGIRPHAVVQLRQDNLAAEHY
                     SMVGFQTRLRWPEQKRIFRTIPGLAEASFVRLGQVHRNCYINAPAVLAETLEARSRPG
                     LFFAGQISGVEGYTESAATGLLCGINAARLATGRPALSLPADTMLGALCHYVSRADAQ
                     DYQPMNVAFGLLPDAPTRARGRRERRLARSAVALAALDAWIARHEAGVAVGDAG"
     gene            15166..16110
                     /gene="xerC"
                     /locus_tag="VD788_05345"
     CDS             15166..16110
                     /gene="xerC"
                     /locus_tag="VD788_05345"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HXV75725.1"
                     /translation="MSPRDPFGQALDAFLRFVEDEKRYSPQTVRAYRSDLRQFRVFLA
                     EHHFKQPPPGPAGIDALAVRGFVAHLSRTGLSKTSIARKLSAVRSFLAFSVRDGRLES
                     NPAEAVPSPRVGKPLPRTLTVDEMFNLLDRIQDVDAISLRDRALLELLYATGLRVSEL
                     VGLDLADLDLAGLTVRALGKGNKERLVPFGRKAAAAVRSWLETSGPARAVGRSPDALF
                     LNARGGRLTDRSVRRILDRRLREAAIVARMSPHAVRHSFATHLLGGGADLRAIQELLG
                     HSSLSTTQRYTHVGTEALMSIYDRAHPRAHRRPRGDGR"
     gene            16107..16910
                     /locus_tag="VD788_05350"
     CDS             16107..16910
                     /locus_tag="VD788_05350"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXV75726.1"
                     /translation="MTTPADRETIAIEAARVGGTIVMRHFRTARATALEEKGLHDFVT
                     EVDHESDRAITALLNERCPDDDVMSEEGAPRVQRKRARWIVDPLDGTTNFAHGIFPFA
                     VSVAVEDEQGLVAGAVYDPLHDEMFHARRRGGARLNGETIRCSRPADSSRALVATGVP
                     FRELSRLDRYLAVFERVARRTAGLRRAGAAAVDLAYTACGRYDGFFEIGLAPWDVAGG
                     ILLVREAGGTVTDLLGGETVLETGDIVAGGSRTHAMLLAITSEVFAGSR"
     gene            complement(16907..17269)
                     /locus_tag="VD788_05355"
     CDS             complement(16907..17269)
                     /locus_tag="VD788_05355"
                     /inference="COORDINATES: protein motif:HMM:NF028518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HXV75727.1"
                     /translation="MVNRKLVRPNLAQLKERMSAAPHVQPGEATRDAGGSVSRRRTAP
                     PDATAAEAFYYLKQMNQKTPMVVVLDDGEQLRGHIEWYDKSCIKVHREGSPNLLVFKH
                     SIRYMYKQEEEGAGHTND"
BASE COUNT         2930 a         5708 c         5911 g         2897 t
ORIGIN      
        1 catccggatc gagtcgacct cgacgaagcc ccaatcgtcg gcacggatcg gccggtcgtc
       61 gcgcgggctc gacaaacggc gttcgagcat ccagctgctg cggtcgttgc gccaggcgtt
      121 ccagtacaga tggaaccaca gctcgctggt cggccgtgtg cggatgttgc gccaggtcag
      181 ccgctgccgg ccggcaagca tgcgcgtcac cgggtcaaga cgcacctcga tcgtgtagtt
      241 cgcgttgcgc ggcgactgcc gatcgagcgc ggcgaccggc gcggccgagg tcagaccggc
      301 caatgcaaga ccggcggcca gtagtcgatc gaccaacgac gctcctcctc ctcgactcag
      361 tcgcacatac tacatcgcga gatcgtcgac ccggcgaggc gggccgcccc taccgatcgc
      421 cccggcgact cgcgagctcg gcctcggccg cgatgtagcc gaaggccgcc cactggtcgt
      481 cggccagcag cgcctcgaac cgctcgtagg cgcgccgctg gtgcccgcga tagagatccc
      541 aattggccac accgtaggcg gtggcggcga actccggccc gtcgccggcg gccgcgcgct
      601 cgagcagctc gccggcatcg cgttggccct tgtacatcaa cagcagctcg tgataggcgt
      661 ggttttcgat cacatccaac gctgcgtcga tcggctcgag cagggcgcgg gcctgttccg
      721 tgcgacccat ccggcgcaag gtcatgtaca tccagtggct ggtcgcgcac agcatgtcgg
      781 ggttggtcgc gagcgccagg cactcgcggt agcagcgcag cgcttgctcg aagtcgccct
      841 cgaggtagta ggcgagaccg aggtggtacc agatgtttcc gtgcagcgtg ctggtcggct
      901 gattgcgcgc gttgggcagc ccgtcgggct cgacgcggtc ttccgtcccc tggatcagct
      961 tcaccgcccg ctcgaggtcg gcgatcgcca ggtcgaactt gcgtacggtg atgtagcgat
     1021 ggccgcggtg gcggtagagt cgcggatcgt cggggaagcg ctcgatcgcg cggtcgtaga
     1081 cctcgatcgc gtcctggtac aggccgaggt aggccgtgcg ccgaccgacc cagatcgcgg
     1141 cctcgacgtc gcccggattc tgttcccagc ggagatgggc ctccttgagt tgacggcggc
     1201 gttcgatctc gacctgctcc ggcagcggcg gagggaacag cggcttgccg agtagcgaga
     1261 agccctgact caccgccggt cgatcgacct cgcccggcgc ccctcccgac gcgagcgcca
     1321 gcagcaccgc gatgacggtc gtcggcacct ctccccccgt tccctcggtc agtcgcctgc
     1381 cggacgccgg tgggccgggt cggcgagaaa ccgcctgtcg gtcaggctgt cgagaaagcg
     1441 tagcaggtcg tcgcgctcgg cggtggtcag cgcgaagccg ccgatgagct cgctcttatt
     1501 ctggttcgca cggccgtcgc ccgcatccgg cccggcgtcc acctcgcgtc cgcccgcggc
     1561 gtagaactcg atcacgccgg ccagcgtccc gatgctgccg tcgtgcatgt acggcgcggt
     1621 gacggcgacg ttgcgcaacg tcggcgcctt gaagcggccc atgtcccggc ggtccccggt
     1681 cgcggcgtac agcccggggt tgtcggccgg gtaggccccg cgcccgtcga cgtcatacag
     1741 cccggtgttg tggaaggtcg gcgcgacgtc ggccgagccc tcgaagacga ccgggccgcc
     1801 gaagttgaaa ccgccgtggc actcggcgca gcgcaaccga tcggagaaga acaaccgcat
     1861 cccccgacgc gtcgcctcgt cgatgccgtc ggtctcgccg tgatagaccc agcggtcgta
     1921 gggcgaatca cccgagatca acgtgcgctc gaacgaggcg atcgcgcggg cgacgttatc
     1981 gagcgtgatc gtctcgccgg cgccgaacgc ggcggcgaac agcgcggggt agcgcgcgtc
     2041 gagtcgcagc cgcgcgagga tgacctgctc gtttccggcg atgcccatct cgatcggcgc
     2101 gtcgttgaac atcggtccgt gtgcctgccg ctcgagcgtg cgcacgtcgg gatcggccca
     2161 gttgaggctc gcgttgtagg cgacgttggc cagcgacatc gcgccgcgcg gatgggtttc
     2221 gcccgtcgcg ccgaccgctc gcgccttgcc gtcggtgaag gccagctcgg ggcggtggca
     2281 ggtcgcgcag gccgtcgtcc cgttcggcga cagccgcgcg tcgtggaata gccggcgccc
     2341 gagctcgacc ttggcctccg acatcgggtt gtgcgccggc acgagcggca cgggaaaacc
     2401 gcgcggaagc cgccacgcga actcggcgcc atcggcgacc gacgccgcga ccaccagcac
     2461 caccaccacg agcggctggc taccagctct tctcaccgag ctgcgacacc ggtgcggtgt
     2521 tgcccgcgat ctcggcccgc tcgtcgaccc aggcgcggaa cgactcgacc gtcgcgccgt
     2581 cgaccgactg gggcggcgcg gcgagccacc gctgcatcgc ggcgagcaac tcgtcggcac
     2641 gggggcgatc gctgccgaac agcgtcaggt aggactcctt cgcctcgtcg atgcggttca
     2701 acccgaggta ggcctcggcg cggtactcga ttgcctccgc gtagttcggc tggatcgaca
     2761 gtgcatgatc gtaggccgcc agcgcgtcgt cgtactcgcc ggtcttgcgg agcgcgtagc
     2821 ccaggctgct cgcggcctgg tacaggttcg ggttgttgcg gagggcgtcc tcgaacgaag
     2881 cgatcgcgcg cgtgtagttc ttctcgatgc gtttttcgag cttcgcgcga tcggcctcgg
     2941 cggcggcggc caactcctct tcgaacttcc aggccttgtc gcggtagacc agcccttcgt
     3001 tgtagaactg gtcggccaac tgctccggtg tcagctccgg cgtcgtcggt tccggctgcg
     3061 cggtgcctcc cgaagcgtgg acggcgagcg ccgcgagcgc cgcgatgatc accgtcgcca
     3121 ccatgcggaa tccccttgct ttcatcgtca ccctcctcga ccctgcgtcg tggtcggtta
     3181 gaatcccgac gccggcattg tagaacgggg ccgggccggc cggcgatcac gacccggcta
     3241 acgaggaaac cttgaacatt cgccgactga cgcgcatcgt cggcacggcg tggctcgccg
     3301 gcgcggcgcc atgggccggt gcgggcgagc cggccgacgg gcgcgagatc gcgcgtgtcg
     3361 cgagcgccga gcagctttgc atccgttggc tcgagacgcg cagcgagatc gagatcgagg
     3421 tctacccgca gattgtcagc atcgcccggc gggtgatcgc cgaggcgttc tcgaacgacc
     3481 tgatcacgcc cggtgcgacg acgaccgcag acgtggcgtg gtacattcgc cacgccgagg
     3541 gcgtgatccg ccgcggcgac gtactgcatc tgtgcaccga cacccaggag atgggctacg
     3601 tgctcgagct cgacgagcag gacgctccgc ccggctcgag ccaggcactc gccgcgggca
     3661 accgctggca ggacttgctg acggaaccgt tccgggcggg tcgcacgggc aacgagatcc
     3721 tcgccagcgt ctacacccac ccgctcgggt tcttgggtca cggcccggga ccgacgatcg
     3781 gcatgtggga cgatcagtgg gtgcagatca agctcgagca gagcgcgctg ttcgacggcg
     3841 agcgcgtgcg ttatctcgcg ggacggcaga cgcgatggca tcgcgtacga tgagcggctg
     3901 gagtcgggcg ctccagctgg ccgaccgcac gcccgcgacg cgcaaccgct acgtagactt
     3961 cctgcgggcg ctgtcgatcg gcgcggtcgt cagcgggcac tggctgatcg cggcgccgtg
     4021 gatgcgcgac gggcagatcc ggctcgacca catgctcggc atgcagccct ggacgcagtg
     4081 gctgacgtgg ctgtttcagg tgatgccgat cttcttcatg gtcggcggct actcgaacgc
     4141 cgcgtcgtgg accgcggcgc gacgggcggg cacgggctac ggcgtttgga cgcgcgcgcg
     4201 gctgcggcgg ctgatcggcc cggtcgcgcc gctcgtcgcg ttctgggccg tggccggtgc
     4261 gatcggctac cgcgccggcg tcccggccga gatgatccgc atcgcctcgc aggcggcact
     4321 cgtgccggtc tggttcctcg cgatctacgt cgtcatcgtg ctgctcgtgc cgtggaccca
     4381 cggcgcgtgg acgcggttcg gcctcgcctc ggtgctcgcg ccggccgcgg cggcggtcgc
     4441 ggtcgacgtg ctgcggttcc gcatgggctg gaccgacctc gcgtgggtca actacctgtt
     4501 cgtttgggcc gcggtacacc agatcggcta cggctggcgc gacgggcggc tgggccacgc
     4561 cggctggcgg ttgatcggca tgctctgcgg cgtggcggcg ctcgccggtc tggtcgcgct
     4621 cgccggctac ccgcacagca tggtcggcgt gccgggcgaa ccggtgtcga acacgctgcc
     4681 gccctccgtc gcgatgctcg cgctcggtct ggcccaggcc gggttgctgt tggcgctcga
     4741 acagccggcg cgacgctggc tgtccggcag acggccgtgg gccgcgacga tcctggtcaa
     4801 cggcacgatc atgacggtct acctgtggca catgacggcg gcgatcctgc tcgtcggtct
     4861 gttgcgactg ttcgacgggt tcggtttgca cgtcgcaccg gggagcacca cgtggtggat
     4921 cacgcggccg gtgtgggtcg cggttctgtc cgtcgcgctc tcgccgttcc tgctggtgtt
     4981 cgctcgattc gagcgggcgg gtgacggggc gccggcccgg catctgcccg gctggcgggc
     5041 catcgtcggc tcggcgctgt tctgcctcgg actggcgctg atcgcgctcg acggggtggg
     5101 cggcggcgga ccgctcggca tccggctgtg ggtcgtgctg ctgccgctcg tcggcgcggt
     5161 cgtcgccggc gcgctaccgg ccgcgcgtgc gcgagtcggc tgacggtcgg aagcggaacg
     5221 cccgcgactc gggccggccg agccattggg ccagcgtctg cgcgttcgcg tccttgtccg
     5281 acagcagcgg gccgtcgatc ggccagtcga tgccgacctg tggatcgtcc cagcggatcc
     5341 ccgactcgca cggcgcgttg taggtgccgg tgcacttgta ctgcacctcg acgatctcgg
     5401 acagcgcgca gaacccgcgg gcgaagccgg ccggggccca gacctgcagc cggttgtcgg
     5461 cggtcgcctc gcgcccgtac caccgcccca acgtcggcga gtcgtaacgg atgtcgacgg
     5521 cgacgacgaa ggcgcggccg accgtcaccc gcatcagctt gcccatcggc gggtcccact
     5581 ggaagtgcaa gccgcgcagc acgcccttcg acgaccgcga gtggttgtcc tggacgaacg
     5641 gttcggtcag accgagttcg gcgaagtggt cgatgcggta ggattccatg aaccagcccc
     5701 gctcgtcgaa gtgggcatgg ggttgcagca cggccacccc atcgaacgga gtcgattcga
     5761 tgacgaccct catcggtgct ccaggccgaa cgcgcgcgcc gcgcgctcgg ccagcgcctc
     5821 gccgatgctc agcgaggccg tggcggcggg tgacggcgca ttgagcacgt gcagcatacg
     5881 ctcgcgctcg atgatgtgaa agtcgtcgag caaccgaccg tcgggagcga cggcctgggc
     5941 gcgcacgccg gccgtgtggg gctcgacgtc gtccggcgac agctgcggta cgagccggcg
     6001 gagcgcgccg acgaatgccg gccgggacga cgaccggcga aactcgtcga gccccgcgcg
     6061 ccagtgacgg gcggcgagcc gccagaaacc gccgtaggac gcgatcgaga gcgcgtcgcg
     6121 cagcgagaag ctgccgcgcg agtagccgtt gcgatcgagc gccagcaccg cgttcgggcc
     6181 ggccgtgact cccccgtcga tcatccgcgt gaagtgcacg ccgaggaagg gaaagcgggg
     6241 atcgggtacg ggatagacca gatgacggat caaccgctcg cggccggggc gcagccggta
     6301 gtactcgccg cggaacggga cgatgcgcag accggggcgc accccgcaca tgcgcgccac
     6361 gcggtcgctc tgcaggccgg cgcagttgac gaggtggcga caggtcagac tcgctccgtc
     6421 gctcgacaga cggaaccgat cgctcagtcg ctcgacgcgc cgcacccgcc aaccggtgag
     6481 caggctcccg ccgcgggtcg acagctcggc ggccatcgcg cgggcgaccc gggtgaagtc
     6541 gacgatgccg gtctggggca ccctcagcgc cgcgacgccg cgcacgtgcg gctcgacgcg
     6601 ctcgagcccc gccgggtcga gccgttcgag cccctgcaac ccgttggccg ttccgcgtcg
     6661 ctcgagctcg tcgagggcgg gaagctgctc gcgcgaggtg gcgacgacga ccttgccgca
     6721 gcactcgtgt gggatgccgt tccgttcgca gaaccgacgg atcgcctcgc gaccgtcgat
     6781 gcagtaaagc gccttcgacg agcccggccg gtagtagaga ccggaatgga ccacgccgct
     6841 gttgtggccg gtctgatgcg tggcgacacg gttttcggcc tcgaccacgg tcgggctcga
     6901 ccgggtgcgg ctcgcgatcg agcgcgcggc cgcgagaccg acgatgccgg ctccgatgac
     6961 cgcgatctcc gtgtccggcg catccatcca gcacccctcg tgacgacccg tcgagcttag
     7021 cgccgggtgt gccggcgatc aaaaaacccg ggtctccggg cgtttcggag aatagcgacc
     7081 aatctgtggc accacggctg cggctcgtat cccggtagct ccgtgggagg cgccgcaccc
     7141 gcgatcgccc tcccgcgtcg gcggtcgcgt ctttttgcgc cgctcatgtt ccgggagcac
     7201 catgactggt gggccatcct tcgcccgatc cgtttttcgg cgacatacgt ctcgtgtttg
     7261 acgctcaccg atcgcgaatc taggtttagg aaggtcacgg aagtgtaatg agtcgagatc
     7321 gaatcgaata caccgccggt ggggcgagtc gtgagcgcgc gacggaggga ccgaacatgc
     7381 caactggcga tcagattatc gcgaaggccc gcgaggctct cgatctcgaa acgtaccggc
     7441 aacagcactg gaccggatcg ttcggtgact acctcgagct gatcaagcgc aacccgcgca
     7501 tcacgcgcac ctcgttcgag cggatctacg acatggtgtt ggcctccggc actcgcgagt
     7561 acatcgacaa caagaagaaa atcatccact tcaacttctt cgacgacgtt gccagcgccg
     7621 gtcaagacgc gatctacggc ctcgatatcg cgctgatgaa actcgtcaac atcttcaaga
     7681 gtgccgccaa gcgctacggc accgaacgcc gggtgatcct gctgcacggc ccggtcggct
     7741 cgtcgaagag cacgatcgcc cgcttgctca agcgcggtct cgaggcctac tcgcgccgtc
     7801 cggagggtgc gctctacacc ttcggctgga tccgcgacct cgaccggccg gatgaggtga
     7861 caccgtgtcc gatgcacgag gagccgcttc acctcgtgcc gatcgatctg cggacgatga
     7921 tcgagaaatc gctcaacgaa gatcacaccg aaggccgcta cacggtgcgg atcgagggcg
     7981 agctctgccc attctgccgg caggaattcc gcgagctgtc cgagcgctac ggcggagact
     8041 ggaccaaggt gatccgccac gtccaggtcc gtcggctgct gctcagcgcg caggaccgca
     8101 tcggcatcgg tacgttccag cccaaggacg agaagaacca ggacagcacg gagctgaccg
     8161 gcgacatcaa ctaccggaag atcgccgagt acgggtccga ttcggatccg cgggcgttca
     8221 acttcgacgg cgagttcaac atcgccaacc ggggcatgat cgagttcatc gaggtgctga
     8281 agctcgacgt ggcgttcctc tacgagctgc tcggcgccag ccaagagcac cagatcaagc
     8341 cgaagaagtt cccgcagacc ttcatcgacg aggtgatcat cggccacacc aacgagccgg
     8401 agtatcgtcg gttgcagaac aacgagtaca tggaggcgct gcgcgaccgc acggtcaagg
     8461 tcgacattcc ctacatcacc aagctgaacg aagagatcaa gatctacgag aaggactaca
     8521 acaaggcgaa gatccgcggc aagcacatcg ccccgcacac gatcgagatg gtggcgatgt
     8581 gggccgtgct gacccggctc gaagacccga agaagcacaa tctgacgctg atgcaaaagc
     8641 tgaagctgta caacggcaaa tcgctgccgg gcttcaccga ggacaacgtc aaggagctgc
     8701 gcaaggagtc gatgcgcgaa ggcatggacg gcatatcgcc acgctacatc caggacaaga
     8761 tcagcaactc gctggtatcg gacgtcagcg aacggtgcat caacccgttc atcgtcctca
     8821 acgagctcga gtcgggcctc aagcatcatt cgctgatcac gagcgaggag cagcgccaac
     8881 gttaccgcga gctgctcgat gaggtgcggc gcgagtacga ggacgtggtc aagaacgagg
     8941 tgcagcgagc gatcgccgcc gacgaatcgg cgatttcgcg gttgtgcggc aactacgtcg
     9001 acaacgtcaa ggcctacacg cagaaggagc gcgtgcagaa cccgtacacc gggcaggacg
     9061 aagagcccga cgagcgcctg atgcgctcga tcgaggagaa gatcgacatc cccgagagtc
     9121 gcaaggacga tttccggcgc gagatcatga actacatcgg cgcgcttgcg ctcgagggca
     9181 agcgtttcga ctaccgcacg aacgagcgct tgcacaaggc gctcgagctc aagctgttcg
     9241 aggaccagaa agactcgatc aagctgacca gtctcgtttc gagcgtcgtc gacaaggaga
     9301 cccaggagaa gatcgacata gtcaagaatc gcctggtgcg ggattacaat tactgtgatt
     9361 cgtgcgcttc cgacgtcctg cacttcgtgg cgagcatctt cgccagaggc gacgtcaaag
     9421 agcgcggctg atgctggaag gcaatgtcgc ggattcagtc agacgtcaat cgcttcaagc
     9481 agatcgttcg gggtcgcgtg cgcgacgagc tgcgcaagca cctcggacag caggagatgt
     9541 tcggccggca aggtaagaac atcgtctcga tcccgatccc gcacctcgag ctgccccact
     9601 tcgcccacga ccccggtgcg ggcaagggcg tgggccaggg cgacggagac ggtgacggcc
     9661 gcaaggccgg caacgaggcc ggccagcact tgctcgaggc cgagttctcc gtcgaggagt
     9721 tggcccacat gctcggcgag gcgctcgagc tgccgcgcat ccgacccaag ggccgcgacg
     9781 agctcgacac caacgccggt cgctacaccg gcgtctcgac ggtcggcccg gaatcgctgc
     9841 gtcactacaa gcgctcgtat cgcaacgcgc tcaagcggct gatcgccacc ggcgcctacg
     9901 accggggccg gccgtcgatc gtgctcgagc gcgtcgacaa gcgctaccgc acgtggaaac
     9961 cgcggcagca accgacggcc aacgccgtga tcgtctacgt gatggacgtt tccgggtcga
    10021 tgggcgcgga ccagaaagag ctggtccgca tcgcgagctt ctggctcgac gcttggatca
    10081 gcagtcatta ccacggcgtg cgcagcgtct acgtcgtgca cgacgccgcg gccaaggaag
    10141 tcgaccgcga taccttcttc cgcattcgcg aatcgggtgg caccgtcatc tcgtcggctt
    10201 acgagctggt ggtgcgcatc cttgccgagc ggttccccgc cgaggactgg aacctgtatc
    10261 tgtttcattt ttccgacggc gagaactaca gccgccaaga caccgaaaag tgcatgcagc
    10321 tgctggacaa gcgcctgttg ccctatctca atctgttcgg ttacggtcag gtggagagct
    10381 acggcaccag cggcgatttc ttcgaggcgg tcgaacgcca ctaccccaac gacgagaagg
    10441 tgacgatggc gcacattccc gatcgtgatg cgatcgtcgg ctgtatcaag accttcctcg
    10501 gcaagggtcg atgatgaagc agaacccgct tccgctcgag ctcgcgctgg cgcaggagcg
    10561 gatcgagaag atcgccgcgg acttcggtct gaccttcttc cgcaccgtct tcgagatggt
    10621 cgactaccgg cagatgaacg cgctggccgc ctatggcggt ttccccacgc gctacccgca
    10681 ctatcgtttc ggcatggagt acaaccagct gagcaagagc tacgagtacg ggttacacaa
    10741 gatctacgag atggtgatca acaacgaccc gacctacgcc tacttgctcg agggcaacac
    10801 catgctcgac caaaagctgg tgatggcgca cgtctacggt cacgccgatt tttttcggaa
    10861 caacatgtac ttctcgcaaa ccaaccgcaa gatgatcgat gcgatggcca accacgcgac
    10921 gcgcattcgt cgctaccagg agcgttacgg tgtcgagcgg gtggagcgct tcatcgacgc
    10981 ctgtctgtcg gtcgaggacc tgatcgatcc cgcctccatc tttcgccgga cgagccctcc
    11041 gcgcgagctg tcggccgggt tggaggaaga ggagacgatg gtgcccggcc gcatgccggc
    11101 caagagctac atggaccgct acgtgaaccc gcccgagtac gtgcgccaac agcgcgagaa
    11161 gctggccaag cgcaaggagg aaacgcgcaa gttcccgcgc caccccgagc gcgacgtgct
    11221 cggtttcatg atggagcatg cgccgctcga caactgggaa cgcgacgtcg tcggtatcat
    11281 tcgcgaggaa gcctactact tcctgccgca gcgacagacc aaggtgatga acgagggatg
    11341 ggcgtcgtac tggcacagca ggatcatgac cggcaaggtg ctgaccgacg ccgaggtgat
    11401 cgattacgcc gaccatcact cgggcacggt cgccacgcag cccggccggt tgaacccgta
    11461 caagttgggg ctcgagctgt ttcgcgacat cgagcgacgc tgggacatgg ggcagttcgg
    11521 cccggcatgg gaggcgtgcg agagcatggc cgagcgcaac aactgggatc gcaagaccgg
    11581 ggccgggcgt gacaagatct tcgaggtgcg tcgaatccac tccgacatca cctttctcga
    11641 cgagttcctg acgcccgagt tctgcgacgc gcagcggctg ttcgtctcga agaacaagga
    11701 cggcaagggg cagaaggccg tcgtcgcgtc ccgcgagttc aaggaggtca agcaggccat
    11761 gctgtttcag ttcagcaacg gcgggcgtcc ggtgatcgag ctggtcgacg cgaactacgc
    11821 caatcgctcc gagctgctgc tgaggcatcg ccacgtcggc gtcgacttga ggtgggactg
    11881 ggccgccgac gtgttggcgc agctcaaccg gatctggcgg cgcccggtgc gactcgagac
    11941 ggcgcgcgac ggaaagtcgg tgcggctcgg tcacgacggg cagaacgtga ccgaggaggt
    12001 cttcgacaag aagaaggccg ccggctcctg acgtcgcgat tccggtcggg gcttaccatt
    12061 ccgcaactcc cctgctcagc cttggttgcg cccgtgcccc acgtgggtta aaaggggacg
    12121 cagggatcga cgagacgggg cgaggatggc cgagacctgt gacaaggcgt tcggtcgcta
    12181 cctgaggaca ctgcgggagc gcagggcact gacgctcgac gccgtcgtct cgttgtccca
    12241 gaccttcccg gaaacgatcc acaagggcta cctctcccgc tgcgagaacg gtttccagaa
    12301 gcccgccttc tccaagatca tcgccctcag tcggatctac gaagtaccgg ccgacgtact
    12361 cgtcgagcgc atggagctcg acatggagct cgatcgcatc ggcggtccgg atacggctgg
    12421 gttgggtttt ggtcagatga cgatccgtgg ccgcgagtct cttcagcgag gatatcgctg
    12481 gcctgcctac gccttcctcc gagatgcagt agtcacagcg agaactgaac cgccgcaatt
    12541 gtcgttccgc gacgtggagg agcagtattc ctgcgcggtg atgagttgcg caactgcggc
    12601 gggcgcgctt ggccggcatc gattcgcgat tcatgagttc aagcatctca agaagcagat
    12661 tcgattgggc ccacgacttc tgactctggt ccttgagcga atctcgtcgt gctacttggc
    12721 gctcggcgac attagtgccg cggagcactt ctctgatcgg gcgattgatg cctctgagca
    12781 ggccggcggt gaggagtatc gtgggcacgc actttacgcg cgtgcgcgcg tggcgctttg
    12841 gtcacaagac cggcgaaaag cagtacgatt attcgaacag gcttatgagg aacacaagag
    12901 ccgtgaccga cgcgaagact gtgcgcttgc gttgaacagc ctcgcgcagt gctacttcga
    12961 tttgcggcgt cggggagcgg ctcgacgttc gtgtcgcgca gcgctgaaga tcgccgattc
    13021 agtggggcat ttgagaacga aggccttcag ccttttgctc ttgggcgagc ttgacgaact
    13081 cgcggagaaa cccaatcttg cgcttcgacg atggagagcg gctgtccaga tcggtaaaga
    13141 gctcaaggat cgtgaactta ggttcaaagc ggagtttctc atattccgcc gcgcaatacg
    13201 ggacgagaat gacgctgttg ccagggctat ccatcggcga ctccatcgac tcgccccgtg
    13261 gatcccccca acgacacaag aactcagcga gttcagaaac ctctctcaca cggtgaccct
    13321 cgctggcgtt tccaggtcgc aacgttagac agttcttcga gcaaccaact gttgctgttg
    13381 tcgttgccag gcggcagcga atcgtcgacc cttgagtcaa gaactgaagg ggggcgagat
    13441 gactttgttc cgactcatgt tgctcgttgt ttctgtcaca ggattcactg caccggcttt
    13501 ttcgggagcc ggagggggtt cccagtcgac ccattgctgg cttcaccctg cggcgagcgt
    13561 ccttggtagg agttccagcc taccggccga agcttgtcag atctcggacg aagccgaccc
    13621 cgttccgact gcgtcgtcgg acggtctgag ctccggggtc cacgcgttcc acacgcccgt
    13681 ctcggtcgtc aagagcgcca ttcgtagagt gtggcagacc ttcgcggctc gttctcgaga
    13741 gtacacagga agatggagcc cggtgcgacg attcgatgag ctatgatgcc tccacggcgg
    13801 tcgaagagga gctcggcgga gtcggtggat ggcggagcgg gttacggtgg tcggcggcgg
    13861 gcttgccgga agcgaggcgg cttggcagat cgcgaagcgt ggggtgccgg tgcggctgtt
    13921 cgagatgcgt cccgtgcggt cgaccgcggt ccatcggacc gaccggctgg cggaactggt
    13981 ttgctcgaac tccctgaaat ccctcgagtt gtcgacggcg cacggcctgc tgaagcagga
    14041 aatgacgcga ttggggtcga tgatcgtcga gagtgcgatg gccaatcgcg ttccggcggg
    14101 gggagcgctg gcggtcgatc gtgagcgatt cgctgaggcc gtgaccgcgc gattgagcgc
    14161 cgagccgttg gtcgagctgg ttcgcgagga ggtccgcgag ataccgccgg acgggatcgt
    14221 gatcctcgcg gtcggcccgc tcgtgtcgga cagcttggcc gcgtcgatcg cgaacttcac
    14281 cgggcaggac tacctgtact tcttcgacgc gatcgctccc gtgatcgagg ccgactcgat
    14341 cgaccgcacg atcgtctacc cgctgtcgcg ctacggcaag ggcgacgggc acgactatct
    14401 gaactgcccg ctcgacgaag cgcagtacca cgccttcgtc cgggagctgg tgtccggcga
    14461 aaaggccccg ctgcacgacg tcgacgcaac accgttcttc gaagggtgcc tgcccatcga
    14521 ggagatggcg cgacgcgggc cggacacgtt ggcgttcggc ccgatgaaac cggtggggtt
    14581 gaccgacccg cgcaccggca tccggcccca cgccgtcgtc cagctacggc aagacaacct
    14641 cgccgccgag cactactcga tggtcggctt ccaaacccgg ctgcgctggc ccgagcagaa
    14701 gcggatcttc cgtaccatac cggggctagc agaggcgtcg ttcgtccggc tgggccaagt
    14761 ccatcgcaac tgctacatca acgcgccggc tgtcctcgcc gagacgctgg aggcccggtc
    14821 gaggcctgga ctgttctttg cgggacagat ctccggggtc gagggctaca ccgaatcggc
    14881 cgccaccggt ttgctgtgcg gtatcaacgc ggcgcgcttg gcgaccggac gtccggcgct
    14941 gtcgctgccg gccgacacca tgctcggcgc cctctgtcac tacgtctcgc gtgccgacgc
    15001 gcaggactac caaccgatga acgtggcgtt cgggctgctg cccgacgcac cgacgcgagc
    15061 ccgtggaaga cgagaacgac ggctcgcgcg gtcggccgtc gcgctcgcgg cgctcgatgc
    15121 ctggatcgcg cggcacgagg cgggcgtcgc ggtgggggac gccggttgag cccgagggat
    15181 ccgttcggtc aggcgctcga cgcgttcctg cgcttcgtcg aagacgaaaa gcgctactcg
    15241 ccccagaccg tgcgcgccta tcgcagcgat ttgcgccagt tccgtgtctt cctcgcggaa
    15301 catcacttca agcaaccacc ccccgggccg gccgggatcg acgcgctcgc ggttcggggg
    15361 ttcgtcgccc acctttcgcg caccggcctg tcgaagacct cgatcgcccg gaaactgtcg
    15421 gccgtgcgct cgtttctcgc gttctcggtg cgcgacggtc ggctcgagtc caacccggcc
    15481 gaagcggttc cgtcgccacg ggtgggcaaa ccgctgccgc gaacgctgac ggtggacgag
    15541 atgttcaacc tgctcgatcg catccaggac gtcgacgcga tctcgctgcg cgaccgcgcg
    15601 ctgctcgagc tgctctacgc gaccggtctc agggtgagcg agctggtcgg actcgacctc
    15661 gccgacctcg acctcgcggg gctgacggtg cgcgccctcg gcaagggaaa caaagagcga
    15721 ctggtcccgt tcgggcgcaa ggcggccgcc gccgtacgtt cctggctcga aacgtcggga
    15781 cccgcgcgcg ccgtcggtcg ctcgcccgac gcgctctttc tcaacgcgcg gggcggacgt
    15841 ctgaccgatc gcagcgtccg ccggatcctc gaccggcgac tgcgcgaggc ggcgatcgtc
    15901 gcccggatgt cgccccacgc cgtccgccat tcgttcgcga cgcacctgct gggcggaggc
    15961 gccgacctgc gagcgatcca ggagctgctc ggccactctt cgctgtcgac gacgcaacgc
    16021 tacacgcacg tcggtacgga agcgttgatg tcgatctacg atcgcgcgca tccgagggcc
    16081 caccgccggc cgcggggcga cgggcgatga ccacgccggc ggatcgcgag acgatcgcga
    16141 tcgaagcggc cagagtcggc ggcacgatcg tcatgcgtca ctttcgcacg gcacgggcga
    16201 cggcgctgga ggagaagggt ctgcacgact tcgtgaccga ggtcgatcac gagtcggacc
    16261 gagcgatcac cgcgctgctc aacgaacgct gccccgatga cgacgtcatg tcggaggagg
    16321 gcgcgccgcg cgtgcagcgc aagcgcgcgc gttggattgt cgacccgctc gacggaacga
    16381 cgaacttcgc gcacggcatc ttcccgttcg ccgtctcggt cgcggtcgag gacgagcagg
    16441 ggctcgtcgc cggggcggtc tacgacccgc tgcacgacga gatgttccat gcgcggcgcc
    16501 ggggcggcgc acggctcaac ggcgagacca tccgttgcag ccggcccgcc gactcgagcc
    16561 gggctctggt agcgaccgga gtgccgttcc gcgagctgtc gcgcctcgac cggtatctgg
    16621 ccgtcttcga gcgcgtggca cggcggacgg cgggattgcg acgcgccggg gcggccgcgg
    16681 tcgacctggc ctacaccgcc tgcgggcgct acgacgggtt cttcgagatc gggctggcgc
    16741 cgtgggacgt cgcgggcggc atcctgctgg tgcgggaagc gggtggaacg gtcaccgacc
    16801 tgctcggcgg cgagacggtg ctcgaaacgg gcgatatcgt tgccggcggc agccggacgc
    16861 atgcgatgct gctcgcgatt acgagcgagg tgttcgccgg gtcacgctag tcgttggtgt
    16921 gccccgcgcc ttcttcttct tgcttgtaca tgtagcggat gctgtgcttg aagacgagga
    16981 ggttggggct gccctcgcgg tggaccttga tgcagctctt gtcgtaccac tcgatgtgcc
    17041 cgcgcagctg ctccccgtcg tccagaacga cgaccatcgg ggtcttctgg ttcatctgct
    17101 tcaggtagta gaacgcctcg gccgcggtcg cgtccggcgg cgcggtacgg cgccggctca
    17161 cgctaccgcc cgcgtcccgc gtcgcctcgc cgggctggac gtgcggtgcg gcggacatgc
    17221 gctccttgag ctgggccagg ttggggcgga ccagtttacg attgaccatc gcaggctccg
    17281 gaatgggtac aaggtctcgc ggtgtcgaac aggtcggcgt gctgggaatt taacacggac
    17341 ctgacgcggc ggcaaccggc aaatacagtt acatttcttt gcacacctgc acctcgaacc
    17401 cctcgggaac acgctgttgg tacttcggcc ggatgcacca ctcggc
//
LOCUS       DASPGN010000188        12234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_616848, whole genome shotgun sequence.
ACCESSION   DASPGN010000188 DASPGN010000000
VERSION     DASPGN010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12234
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616848"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VD788_05360"
     CDS             complement(<1..425)
                     /locus_tag="VD788_05360"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXV75728.1"
                     /translation="MGTNPVDAYLAKTPQPQRKTLTALRRSIRRLLPRSQEGIAYGVP
                     CFKVDGKGVAGFAAFKAHCAYFPMSGSVVGTLKKELSAYRFSKGGIQFATDRVLPPGL
                     VKRLVKARLGELSRPPANGSGPARSYYDNGVMRSRGSYKG"
     gene            complement(454..3813)
                     /locus_tag="VD788_05365"
     CDS             complement(454..3813)
                     /locus_tag="VD788_05365"
                     /inference="COORDINATES: protein motif:HMM:NF015368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HXV75729.1"
                     /translation="MPAHRRSVCSWIAAVGLTSAVWAGSGATDLELAGIPVDRDPWFE
                     YVDAIQPGERLWVGIDPTLLPALGGVSCDIYVVGDRTAAGWIADPSLTDLTSGGPKTH
                     SFCGGTIRTNRVAVAEPGELPSDAGAGLGVAYDVVLDCNRNGLLDAGDFVDGRLDTQR
                     LYALPDTTLSGPLAVASLTFGPAETRQIVYYPADIATRGELPLVVVSHGWTHDYLWYA
                     HIGHHLASYGYVVMSHVNQVGDGGPFATYTAAEETLAATDRLLAIPPGAEVLAGHIDS
                     DRLALIGHSTGGEGVVRAYTMLRNGQASPQRFTTASIRLLVPLAPVSWFAPEVVNPFD
                     VPFHMFGGAADIDVSNGPLDTYVQLLAMFEIAAGPRYLTYIQGAGHGDLHAGQQSSWA
                     DGPDLIGRGRTHEVLLGYLLPMVESYLRGNPAGRDFFTRMDDRFRPEGIDAQVVLAQE
                     MREPEEVGFVIDDFQANDSVDISSSGGQVTYTVSNVAEPSMRDVDGGLDWDGTQPANG
                     MTRSRFAGTDPRCVVFDWGPDAPAYYELEIAIGERGFAAHGFLSFRAAQGTRHPETVA
                     LDGPLTFSVTLRDGTGNQRTLPLAAYGEITQPYQRWGHGIGYGWSNEFSTVRIPLRDF
                     LREGGGLDLGDIAAVRFDFGPSFGSVRGRLALDDIELVGSPPPGPTTAGPAPQPGTPD
                     GDQDGLEDACDNCPAGANADQTDRDGDGVGDACDLCPDLPNPDQSDVDGDGPGDLCDN
                     CPEAANPAQTDSDGDGVGEACDNCSSVANPAQTDGDGDGVGDSCDPCPAAAADDADGD
                     GVCEDTDNCPGVANAEQHDADLDGAGDACDLCPADADDDADQDGWCAELDNCPSTWNP
                     SQQDSEPLAAAIGQWAVSASASSEFTSTDYAAGQAEGAPESPGACVDLPTNWAPRTNS
                     AAPEWLELGYATPVAADGIRVLESLHGGFVFRIELRRTSGGWFTVWEGVDPTDCGAEL
                     VATWPATAFPVDGVRVHTAVAGFEEIDAVALDGRIGQDGVGDACDACPTVFNPSQLAV
                     PFADSLTADAVAFHWSTAADVRYVRGTLSELPLYTYEETGTLASAVALAVAGDSPAAG
                     DGFWYLVRYAGSCGHWQSAPGAEPARDLALP"
     gene            3990..5099
                     /locus_tag="VD788_05370"
     CDS             3990..5099
                     /locus_tag="VD788_05370"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HXV75730.1"
                     /translation="MIPSLAARLPAPTSWVLDLARRHTSAVMFTGGFLFDLATIRRID
                     AWTDLSLQLLYLVGVTTLLLYQHRASLGRWAPPVILERVWAYNVEVLHFFYGALLSAY
                     VVLYLRSSTRARPLVFLILLVALMFVNEMPRVRRAGYRLRLGLYAFCVISFLNYFVPI
                     VLGRMGDWVFALTLAVAAGVTWLVADRLATREVDRRATRARLYSPALAVAVALAVLYA
                     LRLIPPVPLSVQSQGIYHDVRREAASYVLTYEKPPLYLFWRRDSRPFRKQPGDRLHYF
                     LEVFAPARFRQQVYARWEHRDAAGDWRPRDRIRLSIVGGRAEGFRSYAVKSNFEPGQW
                     RVITETADGRAMASLAFTVEEIAGETEREWRRVGG"
     gene            5161..6819
                     /locus_tag="VD788_05375"
     CDS             5161..6819
                     /locus_tag="VD788_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HXV75731.1"
                     /translation="MQRTPGLLRVLSAAGICAAGVPWVPAAEPIETPDARIVEVTVYR
                     DRAEIVRQARVQVPAGASAVEFAGIPAGVEPDSLRVAAEGLPCRLGAVELAGTAGKPE
                     ETPEYLAAQDDVRRLERAVAAATDHERIDSDLKAYLDSLRATATTESARLADGGADPA
                     AIAAVYDLMAGKLEELSARRLERHERLRLLGEELEVARAKLATLAAPAEIRARVATVE
                     IEADRGGSLTLRLAYVVPRSTWRPSYRATLDAATGEVHVVAEGVVSQNTGEGWSGVAL
                     KLSTAAPSQGVQPPHLDPWFLRPASVDLEGGFAERDRFYQNVLTLAPGASEPGAPAEE
                     AVVVESELVRSAYNVAFAVPGVSDVPADGRDHRVVLRAEKLAGQIVYRTVPGMESRAY
                     LTSVTTAPADYPLLAGPVRVFAEGAYLGSFRIEETGPGQELRLPFGADNRIEVVRVPL
                     PRTAGRDGFAGRMREMEYGFRTEMTNRLDRPVTLVLEERLPVSEDERIEVTLGKETTP
                     GHRESERRPGVKIWTLELAPGEKRELLFGYTVRHPKDLYVPGLD"
     gene            complement(6798..7604)
                     /locus_tag="VD788_05380"
     CDS             complement(6798..7604)
                     /locus_tag="VD788_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75732.1"
                     /translation="MAKRILRTWLWIGACTVVSWRLLALARPHPAAEDPAVVRLALAG
                     LGLLFVACGMWAWHRRPGVPTRLFALYGLASGLHWGGPLGIGTSDLQTFELVFYVVAS
                     SVLGGTLLLHLALVFPRRLPPSTLAGAMAVLYAPATIGGLSALGSLAAAGRSLVVVNR
                     VLPLLLVVATAYGYAAAASWIGRLILSPADRRAGSLPLIVTALLGGALPHLVVSSIGP
                     PHELGGLWNLLLAAFPIALAFALIRDRVEPASPPPARGYTTGVAQSSPGT"
     gene            7730..8227
                     /locus_tag="VD788_05385"
     CDS             7730..8227
                     /locus_tag="VD788_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXV75733.1"
                     /translation="MTSDTTRNDHRPSDAIGASPGRFGFGPFTLDRRTGELREDDRVV
                     PLRPLATRALLLLVERRGELVAREELRQCLWGRSAVEWEAGLHQIIRTLRRCLRDDST
                     RPAYIETVPRRGYRFKGRVRVAGAPRSSRRSMWPRTSYFVGGVLTALGSLTVLACLAC
                     LLLAD"
     gene            complement(8255..9925)
                     /locus_tag="VD788_05390"
     CDS             complement(8255..9925)
                     /locus_tag="VD788_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXV75734.1"
                     /translation="MMRCSTTIVSLLCCCATLSGPAPSRAQEGDPPPVERRAEEVFEL
                     LRDGDAGAIRAFVATEMHPSFRELGPVELHLEQLGKMRDTLGVFEVEGIRLTGPVRGE
                     FILRADSGERLVMHVETEPIEPYRLVGLRLKPHEPENRLPAFTSLEQADEELQRLAES
                     NAFSGVVLVARGGRPDWVRAYGMADKERKIPNRPDTRFDVGSITKDFTAVAILQLVEQ
                     GKVELDAPISRYLDGFPPGIAEEVRVIHLLRMESGYGDYYTREFIERREEFVTISDYL
                     EYFKQLSLEFEPGTKRRYSNVGYTILGGIVESVSGMSYPEYLTRHVYGPAGMRSSSFR
                     DLEARQPDYAIGYTNDGPSGRRGFVDTNRSILDVRGNASGGSLSTVGDLLRFQRALRA
                     NELLDPRHTTLLLNGFEESDARPGTRGTAGGAPGVSAVSLEDFDARVNVIVLSNYDEP
                     LAEEAGTNLFRMLRGDTTTDAPRYRVGVMLGVGPEGVTVDSTLPGSPAERAGLRPGDH
                     LRRVNGTPLEGRNVEPLTRAFSRPEEVLLVVDRGGDEIEIRLTPEPAR"
     gene            complement(10080..10934)
                     /locus_tag="VD788_05395"
     CDS             complement(10080..10934)
                     /locus_tag="VD788_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75735.1"
                     /translation="MAASAELVSLLRELKRAGSPIARVKLLARAWRTVRSLSPAERRD
                     VAARVGVAELDGVLDRLGVGGEDGVTPAELLEALESVDGEDRAKLADALRGLGSAEGR
                     RRLLRRSVEIARDLAPAKRPGRKQEAAPAVAAPVEPAAVPTKPPATQTPAAAVRPPSP
                     VAPARVPPPVPRAISPVPAPGGRVAPPSAFDALLARLDAQPALIVRFRLVREFLARAE
                     VGLRRSEIETLLERFAPGWARRRVLAACLLRGSPRSADDARSLIATLPRRADQRWCES
                     ALALRREH"
     gene            complement(10938..>12234)
                     /locus_tag="VD788_05400"
     CDS             complement(10938..>12234)
                     /locus_tag="VD788_05400"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXV75736.1"
                     /translation="RGGGTFVWPAWEQYEFLSLQMMTLDIDLKHVYTVQGIPINIKAV
                     AQVKIGSDIEHISSAAEGFLGMQPEQIRSTIKETVAGHLRGIIGTLTVEELYRDQKRF
                     QDSVRAEAHMDLEGMGFVFKSFTFQEIQDDEGYLNALGQPKIQEALKQARIATANADR
                     DAKIEEEGARQQKEQKRIVVDTEIAESDKALSLKKATIKEEVDVADAKATKAGEMERK
                     VQDIKIAEREVERQKLELNATIRESADAKKYEAERLADAEQYRVERAATAERRRREEA
                     ASAVKAEGLAKAEAESVMRRDIGIAEADAIRAKGEAEAEARRLLAEALKLYNDAGLSI
                     EALKVLPEIAAAVSEPLARAGQTTIISNGGASGGGTGAAKMTEDVVQVLSQLTPIMQQ
                     LAGVDLKTFLQDISKLPGAAVGGKSTLAVPAKKAPETAE"
BASE COUNT         1771 a         4306 c         4283 g         1874 t
ORIGIN      
        1 cccttgtaac tgcctcggga tcgcatcacg ccgttgtcgt agtaggagcg tgccggcccg
       61 ctcccgttgg ccggtggacg ggacagttcc cccagccgag ccttgaccag ccgcttcacg
      121 agcccgggag gcagcacccg gtccgtcgcg aactggattc cgcccttcga gaagcggtaa
      181 gccgacagct ccttcttcag cgtgccgacg acggagccgc tcatcgggaa gtaggcacaa
      241 tgcgctttga aagccgcgaa tccggccacg cctttgccgt ccaccttgaa gcacggcacg
      301 ccgtaggcaa tgccctcctg agaccgcggc aacaggcggc ggatggagcg gcgcagggcg
      361 gtcagcgtct tgcgctgcgg ttgcggggtc ttggcaagat aggcgtcaac ggggttggtg
      421 cccatgcgac gcatcctacg ctcggccccc ggttcagggc aaggcgagat cgcgagccgg
      481 ctcggcgccc ggtgcgctct gccagtgacc gcaggatccc gcgtaacgca ccaggtacca
      541 gaagccgtca cccgccgccg gcgaatcgcc cgcgacggcg agcgccacgg ccgaggcgag
      601 cgttcccgtt tcttcgtagg tgtagagcgg cagctcggaa agcgtgccgc gcacgtaacg
      661 cacgtccgcc gcggtcgacc agtgaaacgc gacggcgtcg gccgtcaggc tgtcggcgaa
      721 cggaacggcg agctgtgacg ggttgaacac ggtcgggcag gcatcgcacg cgtcgcccac
      781 cccgtcctgg ccgatccggc cgtcgagcgc cacggcgtcg atctcctcga acccggcgac
      841 tgcggtgtgc acccgcactc cgtccaccgg aaaggcggtc gccggccacg tcgcgacgag
      901 ctccgcgccg cagtccgtcg ggtcgacacc ttcccacacc gtgaaccacc cgccgctcgt
      961 acggcgcagc tcgatccgaa acacgaatcc gccgtggagc gattccagca cgcggatgcc
     1021 atcggccgca accggcgtgg cgtagccgag ctcgagccac tcgggtgcgg cggaattcgt
     1081 ccgcggagcc cagttcgtcg gcaggtcgac acaggcgccg gggctctcgg gcgctccctc
     1141 cgcctgcccg gcggcgtagt cggtcgaggt gaactcgctg ctggcgctcg ccgagacggc
     1201 ccactgaccg atcgccgccg cgagcggttc ggagtcttgc tgcgaagggt tccaggtcga
     1261 aggacagttg tcgagctcgg cgcaccaccc gtcctggtcc gcgtcgtcgt cggcgtcagc
     1321 gggacacagg tcgcacgcgt cgcccgcgcc gtcgaggtcg gcgtcgtgct gttcggcgtt
     1381 ggcgacgccc ggacagttgt ccgtatcctc gcatacgccg tcaccgtcgg cgtcgtccgc
     1441 ggccgccgcg ggacacgggt cgcacgaatc gcccacgccg tcgccgtcgc catccgtctg
     1501 cgccgggttg gcgacgctcg agcagttgtc gcaagcctcc cccacgccgt cgccgtcgga
     1561 gtcggtctgc gccgggttgg ccgcctccgg acagttgtcg cagaggtcgc ccggaccgtc
     1621 cccgtcgaca tcggactggt cgggattcgg caggtcggga cagagatcgc acgcgtcgcc
     1681 cacgccgtcg ccgtcgcggt cggtctggtc cgcgttcgct cccgcggggc agttgtcgca
     1741 cgcgtcctcc agaccatcct ggtcgccatc gggcgttccc ggctgcggcg ccggaccggc
     1801 cgtcgtcgga cccggcgggg gcgagccgac gagctcgatg tcgtcgagcg ccaaccggcc
     1861 acgcaccgat ccgaacgatg ggccgaagtc gaaccgcacg gcggcgatgt cgccgaggtc
     1921 cagaccgccg ccttcgcgga ggaagtcccg cagcgggatc cgcaccgtcg agaactcgtt
     1981 cgaccaaccg taaccgatac cgtggcccca acgctggtac ggctgcgtga tctcgccgta
     2041 cgccgcgagc ggcagggttc gctggttgcc ggtcccgtcg cgcagcgtga ccgagaaggt
     2101 caacgggccg tcgagcgcga ccgtttccgg gtggcgggtg ccctgtgccg cacggaacga
     2161 gaggaagccg tgagcggcga agccgcgctc gccgatcgcg atctcgagct cgtagtaggc
     2221 gggggcgtcg ggaccccagt cgaagacgac gcagcgcgga tcggttccgg cgaaacgcga
     2281 gcgggtcatg ccgttcgcgg gctgggtccc gtcccagtcg agcccaccgt cgacgtcgcg
     2341 catcgacggc tcggcgacgt tggacaccgt gtacgtcacc tggcctcccg agctgctgat
     2401 gtcgaccgag tcgttcgcct ggaaatcgtc gatgacgaaa ccgacctcct ccggctcgcg
     2461 catctcctga gccaggacga cctgggcgtc gatcccctcg ggtcggaagc gatcgtccat
     2521 ccgcgtgaag aagtcccggc cggcggggtt cccgcgcagg taggactcga ccatcggcag
     2581 caggtagccg agcagcacct cgtgggtccg gccacggccg atcagatcgg gcccgtcggc
     2641 ccacgagctc tgttgtccgg cgtgcaggtc cccgtgcccg gcgccctgga tgtaggtcag
     2701 gtagcgcggt ccggcggcga tctcgaacat cgcgaggagc tggacgtacg tgtcgagcgg
     2761 accgttgctg acgtcgatgt cggccgctcc cccgaacatg tggaacggga cgtcgaacgg
     2821 gttgacgacc tcgggcgcga accacgagac cggtgccagc ggcacgagca agcgaatgct
     2881 cgccgtcgtg aaacgctgcg gagacgcctg cccgttgcgc agcatcgtgt aggcccggac
     2941 gactccctcg cctccggtcg agtgaccgat cagcgccaga cggtccgaat cgatgtgacc
     3001 ggcgagtacc tccgcccccg gcgggatcgc gagcaaccgg tcggtcgcgg ccagggtctc
     3061 ctccgcggcg gtgtacgtcg cgaacggccc gccgtcgccc acctggttca cgtggctcat
     3121 cacgacgtag ccgtaggacg ccaagtggtg gccgatgtgc gcgtaccaca ggtagtcgtg
     3181 cgtccagccg tgactgacga cgaccagcgg cagctcgccg cgagtggcga tgtccgccgg
     3241 gtagtagacg atctgccggg tctccgccgg accgaaggtg aggctggcca cggcgagcgg
     3301 ccccgagagc gtcgtgtcgg gcaaggcgta cagccgctgg gtgtcgaggc gcccgtcgac
     3361 gaagtcgccg gcgtcgagca acccgttgcg gttgcagtcg agcacgacgt cgtaggccac
     3421 gcccaacccc gcgccggcgt cgctcggcag ctctcccggc tcggcgacgg ccacgcggtt
     3481 cgtccggatc gtgccgccgc agaagctgtg cgtcttcggt ccgccggacg tcaggtcggt
     3541 cagtgacgga tcggcgatcc atccggcggc cgtccggtcg ccgaccacgt agatgtcgca
     3601 gctgacccca ccgagcgccg gcaagagcgt cgggtcgatg ccgacccaca gtcgttcacc
     3661 cggctggatc gcgtcgacgt actcgaacca cggatcgcga tcgacgggga tgccggcgag
     3721 ctcgagatcc gtcgcgccgc taccggccca caccgccgag gtcaacccga cggcggcgat
     3781 ccacgagcag acggatcggc ggtgagcggg catcgacacc tccatcgtcc cggagtgtac
     3841 gccgaggcca tcccggtggc gaccgccgcg ccggcctcac gattccgtcg tccgtgcgac
     3901 acgatcgtca tcgtccgtgt tccttgcacc gggcgcggtc gagaccgacg attacgctgg
     3961 accctcgacg accccgctgg agcccgtccg tgatcccctc gctcgccgcc cgattacccg
     4021 cgccgacgtc gtgggtcctc gacctggcgc ggcgtcatac gagcgcggtg atgttcaccg
     4081 gcggcttcct gttcgacctg gcgacgatcc gacgcatcga tgcctggacc gacctgtcgt
     4141 tgcagctgct gtacctcgtt ggcgtcacga cgttgctgct gtatcagcat cgcgcgtcgc
     4201 tcggccggtg ggcaccgcct gtgatcctcg aacgcgtatg ggcctacaac gtcgaggtct
     4261 tgcatttttt ctacggagcc ctgctcagtg cgtacgtcgt gctctatctc aggagcagca
     4321 cgcgcgcgcg gccgctcgtg ttcctgatcc tgctggtcgc actgatgttc gtcaacgaga
     4381 tgccgcgcgt ccgtcgcgcg ggctaccggc tgcgcctcgg cttgtacgcc ttctgcgtga
     4441 tctcgttcct caactacttc gtgccgatcg tgctcgggcg catgggtgat tgggtgttcg
     4501 cgctcacgct cgccgtcgcg gccggtgtga cgtggctcgt cgccgatcgt ctggcgaccc
     4561 gcgaggtcga tcgccgtgcg acgcgcgcgc gtctgtactc gccggcgctc gcggtggcgg
     4621 tcgcgctcgc cgtgctctac gcgctgcgcc tgatcccgcc cgttccgctg tccgtgcagt
     4681 cgcagggcat ctatcacgac gtgcgacgcg aggccgcctc ctacgtgctg acgtacgaga
     4741 agccgccgct ctacctgttc tggcggcgcg actcgcggcc gtttcgcaaa cagccgggcg
     4801 atcggctgca ctacttcctc gaggtgttcg cacccgcgcg attccggcaa caggtctatg
     4861 cccgctggga gcaccgtgac gccgcgggcg actggcgccc gcgcgatcgg atccggctgt
     4921 cgatcgtcgg gggacgcgcc gagggcttcc gctcctacgc cgtcaagtcg aacttcgaac
     4981 ccggtcagtg gcgggtgatc accgagaccg ccgacggtcg cgcgatggcc tccctcgcgt
     5041 tcacggtcga ggagatcgcg ggcgagacgg agcgcgaatg gagacgcgtc ggcggttgac
     5101 ggcattgccg cccgacgccc gcacccaccg cgctatgctg gcccacgaag ggagagtgcg
     5161 atgcaacgaa ctccgggcct tctccgtgtc ctgtccgcgg ccggcatttg cgcggccggg
     5221 gttccgtggg ttccggcggc ggagccgatc gagacgccgg acgcccggat cgtcgaggtc
     5281 accgtctacc gcgaccgcgc cgagatcgtg cgccaggcgc gggtgcaggt gccggctggg
     5341 gcgtcggcgg tcgaattcgc cggcatcccc gcaggcgtgg agccggactc gttgcgggtc
     5401 gccgccgagg ggctgccctg ccggctcggt gcggtggagt tggccggcac cgccggcaag
     5461 cccgaggaga cgccggagta cctggccgct caggacgacg tccgccgcct cgagcgcgcc
     5521 gtcgccgccg ccacggacca cgaacggatc gacagcgacc tgaaggccta tctcgacagc
     5581 ctgcgcgcga cggccaccac cgagtccgcc cggctggcgg acgggggcgc cgatccggct
     5641 gcgatcgccg cggtttacga cttgatggcc ggcaagctgg aagaactgtc tgcccgacgg
     5701 ctcgagcgtc acgagcggct gcgcctcctc ggcgaggagc tcgaagtcgc tcgggcgaag
     5761 ctcgccacct tggccgcgcc ggccgagatc cgggcacggg tggcgacggt cgagatcgag
     5821 gcggaccgcg gcggatcgtt gacgttgcgg ctggcctacg tcgtgccacg ctccacgtgg
     5881 cggccgagct accgcgcgac cctcgacgcg gccaccggcg aggtccacgt ggtggccgag
     5941 ggggtggtga gccagaacac gggcgagggt tggagtggcg tcgcgttgaa gctgtccacg
     6001 gcggcgccga gccaaggtgt gcaaccgccc cacctggacc cgtggttcct gcggccggca
     6061 agcgttgacc tcgaaggagg tttcgcggag cgggatcgct tctatcaaaa cgtcctgacc
     6121 ctcgccccgg gcgcttccga acccggggcg ccggccgagg aagcggtggt cgtcgagtcc
     6181 gagctggtcc gatccgccta caacgtcgcg tttgccgtgc cgggggtttc cgacgttccg
     6241 gcagacggtc gggatcatcg cgtcgtcctg cgcgccgaga agctcgccgg ccagatcgtg
     6301 taccgcaccg tgcccggaat ggagagccgg gcctacttga cgtccgtgac caccgccccg
     6361 gcagactacc cgctgctcgc cggccccgtg cgcgtattcg ccgagggcgc ctacctgggg
     6421 tcgttccgca tcgaggagac cggccccggt caggagctgc ggctgccctt cggggccgac
     6481 aaccggatcg aggtggtgcg cgtgccgctg ccccggacgg cgggccgcga cggcttcgcg
     6541 ggccgcatgc gcgagatgga atacggcttc cgcaccgaga tgaccaatcg gctcgatcgc
     6601 ccggtgacgt tggtgctcga ggagcggctg cctgtctcgg aggacgagcg gatcgaggtg
     6661 acgctgggca aggagaccac gccgggccac agggagagcg agcgccggcc gggcgtcaag
     6721 atctggacgc tcgagctcgc gccgggcgag aagcgcgaac tgctgttcgg gtacacggtg
     6781 cgccacccga aggatctcta cgtgccgggg ctcgactgag ccacgccggt ggtgtagccg
     6841 cgcgcgggag gcgggctcgc gggctccaca cggtcacgga tcagcgcgaa ggccagcgcg
     6901 atcgggaacg cggcgaggag caggttccac aaaccaccga gttcgtgtgg cggaccgatc
     6961 gacgacacga cgagatgtgg cagcgctcca cccagcaggg cggtgacgat caggggcagg
     7021 ctgccggcgc gccgatccgc cgggctgagg atcaaccgtc cgatccagga cgcagccgcc
     7081 gcgtacccgt aggccgtcgc cacgaccaag agcaacggaa ggacacggtt cacgaccacg
     7141 agagaccgcc cggctgcggc gagcgaaccc agcgccgata ggccaccgat cgtcgccggt
     7201 gcgtacagca ccgccatcgc tcccgccagc gtggacggcg gcagtcgtcg agggaagacg
     7261 agcgccaagt ggagcagcag cgtcccgccg agcacgctgc tcgcgacgac gtagaacacc
     7321 aactcgaacg tctgcaagtc cgacgtgccg atcccgagcg gccctcccca atggagaccc
     7381 gaggcgagac cgtacagtgc gaacagtcgg gtcgggacgc ccggccggcg gtgccaggcc
     7441 cacatcccgc acgcgacgaa cagcaggccg agcccggcga gcgcgagccg caccacggct
     7501 ggatcctcgg cggccgggtg aggacgggcg agggccagga gccgccagga aaccaccgtg
     7561 caggcaccga tccaaagcca cgtgcgcagg atccgcttcg ccatcgtgag cacctccacg
     7621 cgtgtgttac ggccgtccgt ccggttcgtc ccgcgtggac ccctgacgaa accctgaaaa
     7681 aagggagatt cgtcgacggc ggcgggccgc gtacaataaa gaactcggaa tgaccagcga
     7741 tacgacgcgg aacgatcatc gaccgtccga cgcgatcgga gcatcgccgg gccggttcgg
     7801 cttcggcccg ttcaccctcg atcgtcgtac gggcgagctg cgcgaggacg accgcgtcgt
     7861 gcctctgcgt ccgctggcca cgcgcgcgtt gctcttgctc gtcgagcgcc gaggcgagct
     7921 ggtcgctcgc gaggagctga ggcagtgcct gtggggccgc agcgccgtgg aatgggaggc
     7981 gggtctgcac cagatcatcc gtacgctgcg gcgctgcctg cgggacgatt cgacgcggcc
     8041 ggcttacatc gagaccgtcc cccggcgtgg ttatcgcttc aagggccgcg tgcgggtcgc
     8101 gggcgctccg cgttcgtcgc gtcgttcgat gtggccgcgg acgagctact tcgtcggcgg
     8161 agtgctcacg gcgctcggct cgctgaccgt gctcgcgtgc ctcgcttgcc tgctcctcgc
     8221 cgactgacgc cgacgtcagg tcctggaacg agtctcagcg tgcgggctcg ggcgtcagcc
     8281 ggatctcgat ctcatcgcct ccgcgatcga cgaccaagag gacttcctcc ggtcgcgaga
     8341 acgcacgcgt cagcggctcc acgttgcggc cttcgagcgg cgtgccgttg acgcgacgca
     8401 ggtggtcgcc cggtcggaga cccgcgcgct ccgccggaga gccgggcagc gtcgagtcga
     8461 ctgtcacccc ctccggcccg acgccgagca tcacgccgac gcggtagcga ggagcatcgg
     8521 tcgtcgtgtc gccgcggagc atgcgaaaca ggttcgtccc cgcctcctcg gcgagcggtt
     8581 cgtcgtagtt cgacagcacg atcacgttga cacgggcgtc gaagtcctcg aggctgaccg
     8641 ccgagacgcc gggggccccg ccggcggtcc cacgcgtccc cggtcgcgcg tcggactctt
     8701 cgaacccgtt gagcagcagg gtcgtatggc gcgggtcgag cagctcgttc gcacgcaacg
     8761 cgcgttggaa gcgcagcagg tcgccgacgg tcgacaggct gccgccggag gcgttcccgc
     8821 ggacgtcgag gatcgagcga ttcgtgtcga cgaagccgcg gcgtccggac gggccgtcgt
     8881 tcgtgtagcc gatcgcgtag tccggctgcc gcgcctcgag atcgcgaaac gacgaagatc
     8941 gcattccggc cgggccatag acgtgacgcg tcaggtattc cgggtagctc attcccgaca
     9001 ccgactcgac gatgccgccg aggatcgtgt agcccacgtt gctgtagcgc cgtttcgttc
     9061 cgggctcgaa ctcgagcgag agctgcttga agtactcgag gtagtccgag atcgtgacga
     9121 actcctcgcg acgctcgatg aactcgcgcg tgtagtagtc gccgtatccc gactccatcc
     9181 gcaggaggtg gatcacccgc acctcctcgg cgatccccgg tggaaagccg tcgagatacc
     9241 ggctgatcgg ggcgtcgagt tccacctttc cctgctcgac gagctgcagg atcgcgaccg
     9301 cggtgaagtc cttggtgatc gagccgacgt cgaaccgcgt gtcgggccgg ttcgggatct
     9361 tccgctcctt gtcggccatg ccgtaggccc ggacccaatc cggccggccg ccacgggcga
     9421 cgagcaccac gcccgagaaa gcgttggatt ccgcgagacg ctgcagctcc tcgtcggcct
     9481 gctcgagact ggtgaacgcc ggcagacggt tctccggctc gtgcggcttc agccggagcc
     9541 cgacgagacg gtagggctcg atcggctcgg tctcgacgtg catgacgagc cgttcgccgg
     9601 aatccgcgcg gaggatgaac tcgccgcgaa cgggtccggt gagtcggatc ccttcgacct
     9661 cgaacacccc gagcgtgtca cgcatcttgc ccaactgctc gagatgcagc tcgaccggtc
     9721 ccagctcgcg gaacgaaggg tgcatctccg tcgccacgaa cgcgcgaatc gccccggcgt
     9781 cgccgtcgcg cagcagctcg aacacttcct cggcccggcg ctcgaccggg ggcgggtcgc
     9841 cctcctgggc ccgagacgga gccggcccgg acagcgtcgc acagcagcag agaagcgaaa
     9901 cgatcgtggt cgagcatctc atcatcaccc ccgtcggtac cgcgtccttc gcgcgaggcg
     9961 aaactagcaa cggccgtgcc atgcggcgga ccgcgattgc gcgtcgttcg ggcgggtcag
    10021 gccgccccaa ttggggtgcc gccaccccaa gacgagcgtg cggggctctc ctcggcgtct
    10081 cagtgctcac ggcggagggc gagcgcgctc tcgcaccagc gctggtccgc gcggcggggc
    10141 agtgtcgcga tcagcgagcg cgcgtcatcg gccgagcgag gggagccgcg cagcagacag
    10201 gcggcgagta cccggcggcg ggcccagccg ggcgcgaagc gctcgagcag ggtctcgatt
    10261 tcgctacggc ggagcccgac ctccgcccgg gcgaggaact cgcggaccag ccggaagcgc
    10321 acgatcagcg cgggctgggc gtcgaggcgg gccagcaacg cgtcgaacgc cgacggcggc
    10381 gccacacgac cgcccggcgc cgggacgggc gaaatggcac gtggaacggg gggcggcaca
    10441 cgcgcgggcg ctactggcga cggcggacgg accgcggcgg ccggggtctg cgttgcgggc
    10501 ggtttcgtcg gcacggcggc gggctcgacg ggggcggcga ccgcgggtgc cgcctcctgc
    10561 tttcggcccg ggcgcttcgc aggtgcgagg tcgcgcgcga tctcgacgct ccgccgcagc
    10621 agcctcctcc gtccctcggc actgccgagc ccgcgcagcg cgtcggcgag cttcgcccgg
    10681 tcctcgccgt cgacgctctc gagcgcctcg agcagctcgg ccggcgtcac gccgtcctcg
    10741 ccgccgacgc ccaaccggtc gagcacgccg tcgagctcgg ccaccccgac gcgggcggcg
    10801 acgtcgcgcc gctcggcggg cgagagcgag cgtacggtgc gccaggcgcg agccagcagc
    10861 ttgacgcgcg cgatcgggga cccggcgcgc ttcaactcgc gcagcagcga cacgagctcg
    10921 gccgaggcgg ccatcggcta ctcggcggtc tccggagcct tctttgccgg cacggcgagg
    10981 gtcgacttgc ccccgaccgc ggcgccgggc agcttcgaga tgtcctgcag gaacgtcttc
    11041 aggtcgaccc cggcgagctg ctgcatgatc ggcgtgagct ggctcaacac ctgcaccacg
    11101 tcctcggtca tcttggcggc gccggtgccg ccgccgctcg cgccgccgtt gctgatgatc
    11161 gtcgtctggc cggcgcgggc gagcggctcg gacacggccg ccgcgatctc gggcaacacc
    11221 ttcagcgcct cgatcgacag gcccgcgtcg ttgtagagct tcagcgcttc cgccagcagg
    11281 cgccgggcct cggcctcggc ctcgcccttg gcgcggatcg cgtcggcctc ggcgatgccg
    11341 atgtcgcggc gcatgaccga ttcggcctcg gccttggcca gaccctcggc cttcaccgcg
    11401 gacgcggcct cctcgcggcg gcggcgctcg gcggtggcgg cgcgctcgac gcggtattgc
    11461 tcggcgtcgg ccaggcgctc ggcctcgtat ttcttggcgt cggcgctctc gcggatcgtc
    11521 gcgttcaact cgagcttctg ccgctcgacc tcgcgctccg caatcttgat gtcctgcacc
    11581 ttgcgctcca tctcgccggc cttggtcgcc ttggcgtcgg cgacgtcgac ctcctccttg
    11641 atcgtcgcct tcttcaggct gagcgccttg tcggactcgg cgatctcggt gtcgaccacg
    11701 atgcgcttct gctccttctg ctgccgcgcc ccctcttcct cgatcttcgc gtcgcggtcg
    11761 gcgttggccg tcgcgatgcg cgcctgcttc agcgcttcct ggatcttcgg ctggccgagc
    11821 gcgttcagat agccctcgtc gtcctggatt tcttggaacg tgaaggactt gaacacgaag
    11881 cccatgccct cgaggtccat gtgggcctcg gcgcgcacgc tatcctggaa ccgcttctga
    11941 tcgcggtaca gctcctcgac ggtcaacgtg ccgatgatgc cgcgcaggtg gccggcgacg
    12001 gtctccttga tcgtcgagcg gatctgctcg ggctgcatgc cgaggaagcc ttcggcggcg
    12061 gacgagatgt gctcgatgtc gctgccgatc ttcacctgcg cgacggcctt gatgttgatc
    12121 gggatgccct gcaccgtgta gacgtgtttc aggtcgatgt cgagcgtcat catctgcagc
    12181 gacaggaact cgtactgctc ccaggcgggc cagacgaacg tgccgccgcc acgg
//
LOCUS       DASPGN010000189         8192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1085133, whole genome shotgun sequence.
ACCESSION   DASPGN010000189 DASPGN010000000
VERSION     DASPGN010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8192
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085133"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..915)
                     /gene="dprA"
                     /locus_tag="VD788_05405"
     CDS             complement(<1..915)
                     /gene="dprA"
                     /locus_tag="VD788_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXV75737.1"
                     /translation="MDRTPTIDWIALNLMPGLGPVSAHRAVARYGHPATVAYHLPLDR
                     LFDGLRLGAEERERLRQARPRLRRQAERELRLAERSGVRVVGHGDPDYPAALAELADA
                     PLVLYLRGALAPGVTRVAVVGSRTPTAYGRRVAIGLAAQLAVRGIEVVSGGARGIDCL
                     AHRSVLDEGHRTVAVLGSGLLRPYPDENVGLYDEVAAAGAVLSEFPLHYPPRADNFPR
                     RNRIISGLSAAVIVVEAAERSGALITARAALEQGREVLAIPGPIDSHKSVGCNRLIQQ
                     GAKLVQGVDDVLDELSPMYRSAVGRSPAE"
     gene            1080..3677
                     /locus_tag="VD788_05410"
     CDS             1080..3677
                     /locus_tag="VD788_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HXV75738.1"
                     /translation="MNGKIVVTLILAAILAAPALALDSAGYLPDDADHDPDLPPPESF
                     LGFEVGRRHLRHDQLVGYLELLAERSPRLAIERTGTTHEGRPLLLLTVSSPANLARLD
                     GIARGRADGSTGGPLVVWHGFSVHGDEASGSNAALLLAYHLAAARDAATTRLLDRTVV
                     LIDPSLNPDGMARFAQWSASHWAHAPSADPAARAHHEAWPGGRTNHYWFDLNRDWLLL
                     VHPESRARVARFHHWRPHVLTDFHEMGSDATYFFQPGVPSRMHPLTPERNIELTREIG
                     SYHAAALENLGQLYFTEEVFDDYYYGKGSTYPDVNGAIGILFEQASSRGQRIDTPHGQ
                     LDFETTVRNQLATALSTLRAADDLRDELIAYQAAFYDDAAERARQDPVGAYVIGDAGD
                     PARAYHLVELLLRHQIEVYELARELTVAGRSFAPNAAWVVPTAQRQYLLVHAMMERRT
                     EFLDQTFYDVSAWTLPLSFNLPVATLGRSRVGGATGARVRDVAFPAGAFVPDGAAYAH
                     AFDWSGYYAPRALERLLASERRVYVATKPFTAETSTGRHRFDRGSIVIPAASNGGVGA
                     LEELLSSVAERDGIDVRVLSGGLTVEGVDLGSPSLRRLEPVRPLLVVGPGASAYEAGQ
                     VWHLLDTRVGLPLTMVDGFRLDQVNLAEFSELILVDGDYAKLGDEVVERVRGWIRGGG
                     HLIAIKRAARWAVDGGLFARPADEDGEDEAQDDDAQEDEPDAEPAAEEKSRRYDDFRD
                     DRARRRIAGSIFAVELDATHPVGFGYEPGRLPVFRNETVIVEPSDNPYENVAIYAADP
                     LLSGYVGEERLAELAGSAAIVARRIGRGSVVLMADDPNFRGIWYGTNRLFINAVYFSQ
                     IIDETELDD"
     gene            3687..6686
                     /locus_tag="VD788_05415"
     CDS             3687..6686
                     /locus_tag="VD788_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HXV75739.1"
                     /translation="MRPTRPTRFLPLAIAIAWTAIVPAAAEIEQIEGLPLETLFPDAR
                     FDPAVPSPHDVFGFHPGARPLRHAEVLGYLRTLADTSPRATLVEYARTHEGRELVYLA
                     VADERAIADLERFRDEHRARVDPRGRPAAEDAARLDGARAVAWIAYGIHGDELSSVDA
                     AVAVAYRLVAGTDPLSLRLRDELLVLIDPIENPDGRDRYLAMTQAFAHRRPTADLDDL
                     SHNAVWPWGRGNHYLFDLNRDWFTMVQPESRRSEVIASWNPQLVVDSHEMGSNDTYLF
                     SPARHPFNPYLPESQAAWGERFAADQARALDRHGYPYYTREWNEEFFPGYGSSWASYL
                     GAAGILYEMSGTEGTLVKTRDGRLRTYPQAVHHQTTSTLANLTTLADNRSEILRDFVA
                     DRRKTIARAGKEWPAAWILPRGRHPARVDRLVDLMLRQSIEVEFGDAHVPGLIDSRTG
                     ETAPSDALTEPFYVVRLDQPAAPLARQLLDPHVPMNAEFFREEREHLERGKGTRLYEV
                     TAWSLPLAYDVDAYWTETAPAREGFTAARPQPAAGGMAFVEAPVGYLIDGLSDGSLPL
                     LVELLEQGITPRVAEKPFRVAGRDYPRSTLFVLAEGLPADLGERLAAAGERHGVVVRA
                     VPTIKAERGPDLGGGHFQPLRQPRVGVWTGWPVSPPAYGAIWNLLDRDLGLRFNALDV
                     ARAQSFDLTRYNVLVFPPTFAGQDAYARVFGEAGLARLKRWIEGGGTAIGVGAGAEFL
                     ADAGHGLTATRLRRQALDDYPPVVLGPDAERAERAGAFRAVGLRAPETRDDDDEDAKT
                     KTKTKSKTEAETAAAAAPPYDVAPVLGPGARPFARGHEQGTPAAETPVDLAHWMASWL
                     APGRDKPDTEELERADRRLRLFSPRGAYLRLELDENVWLTWGLPGELPALVRASDTLV
                     AEPPVQVAARFAPIDRLHLGGLLWPEAAGRLALTAYATRESLGRGQVVLFLSEPEFRG
                     FTLGTRRLLVNAILYGPGLGTEWSNPW"
     gene            6704..7516
                     /gene="lsrF"
                     /locus_tag="VD788_05420"
     CDS             6704..7516
                     /gene="lsrF"
                     /locus_tag="VD788_05420"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: protein motif:HMM:NF006081.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HXV75740.1"
                     /translation="MDWGMQNRLSRILDPRSGRTVMLAIDHGYFLGPTSGLEAPGRTC
                     APLLPYADSLMLTRGTLRQCIPANSNVPIVLRVSGGTSILRELSNEGLTVAIEDAIRL
                     NASAVTLSVFVGSEGERTTLLNLAKLVDYGQQYGVPVLAVTAVGKEMTRDARYLGLAC
                     RISAELGAHFVKTYYCDGFDEVVAATPVPIVIAGGKKIAEREAIELAAKAVRAGASGV
                     DMGRNIFQSDCPVGMIRAVRSVVHDGASATHAFEIYREQKEAAGAGSGLLQQ"
     gene            7488..>8192
                     /locus_tag="VD788_05425"
     CDS             7488..>8192
                     /locus_tag="VD788_05425"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXV75741.1"
                     /translation="MRAAVYYSNNDLRLEERPTPVVGPGELLMRVEASGICGTDLIEW
                     YRKPKAPIVLGHEVAGTVVEIGAGVDRFVDGDRIVTTHHVPCNSCRFCLTDRHSVCPT
                     LHATTFDPGGFAEFVRIPAINVERGTFHLPDTVSFEQGSFVEPLACCIRAQRLARVAP
                     GDSVAVLGAGVAGILMIKLARALGAGRVIATDTSDARAGLAVAAGADVALSADDPECI
                     DRAIASNYARRIDRVLV"
BASE COUNT         1174 a         2989 c         2861 g         1168 t
ORIGIN      
        1 ctcggccggc gagcgaccga cggcggagcg gtacatcggg gacaattcgt cgagcacgtc
       61 gtcgacgccc tgcacgagct tggcgccctg ttggatcagt cggttgcagc cgaccgactt
      121 gtgcgagtcg atcgggcccg ggatcgccag cacctcccgg ccttgctcga gggcggcgcg
      181 tgcggtgatc agcgcccccg accgctcggc ggcctcgacc acgatcaccg ccgcggacag
      241 tccgctgatg atgcggttgc gtcgcgggaa gttgtcggcg cgcggcgggt agtgcagcgg
      301 gaactcgctg aggaccgcac ccgccgcggc gacctcgtcg tacagcccga cgttctcgtc
      361 gggatagggt cgcaacaggc cggagccgag gactgccacc gtgcggtggc cttcgtcgag
      421 caccgagcga tgggccagac agtcgatgcc gcgtgccccg cccgacacga cctcgatgcc
      481 gcggacggcg agctgcgcgg ccaggccgat cgcgacgcga cgcccgtagg ccgtcggcgt
      541 gcgcgagccg acgacggcga cccgggtcac gccgggggcc agcgctccgc ggaggtagag
      601 cacgagcggc gcatcggcga gctcggcgag cgcggccggg taatcggggt cgccgtggcc
      661 gacgacgcgc acccccgacc gctccgccag gcgtagctcg cgctcggcct gacggcgcaa
      721 gcgcgggcgc gcctgtcgca gccgttcccg ttcttccgcg ccaagacgca ggccgtcgaa
      781 cagacgatcg agcggcaggt ggtacgccac cgtcgcggga tggccgtagc gggcgaccgc
      841 ccgatgcgcc gagaccggtc cgagccccgg catcaggttg agcgcgatcc agtcgatcgt
      901 gggcgtgcga tccaacgccg cgtcaccggg gaggccggtg tccagtatgg aaggccgacc
      961 cggccggcgg ccagccgtcg agccgtgccg cgggttgccg atccgaaccc ggccggccga
     1021 ccgccaggcc cacgtgtgct ttaatgcgcc atccccacgc tgactgaggt gcggagcgga
     1081 tgaacggcaa gatcgttgtg acgctgatcc tcgcggcgat cctcgcggct cccgcgctcg
     1141 ctctcgattc ggccggctac ctgcccgacg acgccgacca cgaccccgac ctcccgccac
     1201 ccgagtcctt cctcggtttc gaggtgggtc gccgtcacct gcgccacgac cagctcgtgg
     1261 gctatctcga gcttctcgcc gagcggtcgc cgcggctcgc gatcgagcgc accggcacga
     1321 cccacgaggg ccgcccgctg ctgctgctga ccgtctcttc gccggccaac ctcgcgcggc
     1381 tcgacgggat cgcgcggggc cgcgccgacg gatcgaccgg cggcccgctc gtcgtctggc
     1441 acgggttctc ggtccacggc gacgaggcca gcggttcgaa cgccgcgttg ctcttggcct
     1501 accatctcgc cgccgcacgc gatgccgcga cgacccggct gctcgaccga accgtcgtgc
     1561 tgatcgaccc cagtctcaac cccgacggca tggcgcgctt cgcccagtgg tccgcctcgc
     1621 actgggccca cgcgccgagc gccgacccgg cggcgcgcgc gcatcacgaa gcgtggccgg
     1681 gggggcgcac caaccactac tggttcgacc tcaaccgcga ttggctgctg ctcgtccatc
     1741 cggaatcgcg cgcgcgcgtc gcccgcttcc accactggcg tccccacgtg ctgaccgact
     1801 ttcacgagat gggcagcgac gcgacctact tcttccagcc cggcgtgccg agccggatgc
     1861 acccgctgac ccccgagcgc aacatcgagc tgacgcgcga gatcggcagc taccacgccg
     1921 ccgcactcga gaatctcggc cagctctact tcaccgaaga ggtgttcgac gactactact
     1981 acggcaaggg ctcgacctac ccggacgtca acggggcgat cggtatcctg ttcgagcagg
     2041 ccagctcgcg ggggcagcgg atcgacacgc cccacggcca gctcgacttc gagacgaccg
     2101 tgcgcaatca gctggcgacg gcgctgtcga ccctgcgcgc ggccgacgac ctgcgcgacg
     2161 agctgatcgc ctaccaggcg gcgttctacg acgacgccgc cgagcgggcg cgccaggacc
     2221 cggtcggcgc ttacgtcatc ggcgacgccg gcgatccggc gcgcgcctac cacctggtcg
     2281 agctgctgct ccgccaccag atcgaggtgt acgagctggc gcgcgagctg accgtcgctg
     2341 gccggagctt cgcgccgaac gcggcctggg tcgtcccgac cgcacaacgc cagtacctgt
     2401 tggtccacgc gatgatggag cgtcgcaccg agttcctaga ccagacgttc tacgacgtct
     2461 cggcgtggac cctgccgctg tcgttcaacc tgccggtggc gacactcggc cgctcgcggg
     2521 tcggcggcgc gaccggcgcg cgcgtccgcg acgtcgcgtt tccggccggc gccttcgtgc
     2581 ccgacggggc ggcctacgcc catgccttcg actggagcgg ctactacgcg ccacgcgcgc
     2641 tcgagcggct gctcgcgagc gagcgacggg tctacgtcgc gacgaagccg ttcacggcgg
     2701 agacctcgac cggccgccac cgcttcgacc gcgggtcgat cgtcatcccc gccgcgtcga
     2761 acggcggcgt cggcgcgctc gaggagctgc tctcgtccgt cgccgagcgg gacggcatcg
     2821 acgtccgcgt gttgagcggc ggcctgacgg tcgagggcgt cgatctgggc tcgcccagcc
     2881 tgcgccgcct cgagccggtg cgtccgctgc tcgtcgtcgg acccggcgcc agcgcttacg
     2941 aggccgggca ggtctggcac ctgctcgaca cccgcgtcgg gctgccgctg acgatggtcg
     3001 acggctttcg cctggatcag gtcaatctcg ccgaattctc cgagctgatc ctggtcgacg
     3061 gcgactacgc caagctcggc gacgaggtcg tcgagcgggt ccgcggctgg atccgcgggg
     3121 gcggacatct gatcgcgatc aagcgcgccg cgcgctgggc cgtcgatggc ggactgttcg
     3181 ctcgcccggc ggacgaggac ggcgaggacg aagcacagga cgacgacgcg caagaggacg
     3241 aaccggacgc cgaaccggcc gcggaggaga agtcgcggcg ctacgacgac ttccgcgacg
     3301 atcgcgcgcg gcgacgcatc gcgggcagca tcttcgcggt cgagctggac gccacgcatc
     3361 ccgtcggctt cggctacgag cccggcagac tgccggtctt ccgcaacgag acggtgatcg
     3421 tcgagccgag cgacaacccg tacgagaacg tcgcgatcta cgccgccgac ccgttgctca
     3481 gcggctacgt cggcgaggag cgtctggccg agctcgccgg cagcgcggcg atcgtcgcgc
     3541 gccgcatcgg ccggggctcg gtcgtgctga tggccgacga ccccaacttc cgcggcatct
     3601 ggtacggcac caaccggctg ttcatcaacg ccgtctattt ctcacagatc atcgacgaga
     3661 cggagctcga cgactgagga gcgttcatgc gacccacccg accgacccgg ttcctgccgc
     3721 tcgccatcgc gatcgcctgg accgcgatcg tgcccgcggc tgccgagatc gagcagatcg
     3781 agggcctgcc gctcgagacg ctgtttcccg acgcgcggtt cgacccggcg gtcccttcgc
     3841 cgcacgacgt cttcggcttc caccccgggg cacgcccgtt gcgtcacgcc gaggtgctcg
     3901 gttacctgcg gacgctggcc gatacctcgc cgcgcgcgac gctcgtcgag tacgcgcgta
     3961 cgcacgaagg gcgcgagctg gtctatctcg ccgtcgccga cgagcgcgcg atcgccgacc
     4021 tcgagcgctt tcgcgacgag caccgagcgc gggtcgatcc gcgcgggcgc cccgccgccg
     4081 aggacgcggc gcggctcgac ggggcgcgcg cggtggcctg gatcgcctac ggcatacacg
     4141 gcgacgagtt gagcagcgtc gacgccgcgg tcgccgtcgc ctaccggctg gtggccggga
     4201 ccgatccgct gtcgctgcgc ctacgcgacg agctgctggt gctgatcgac ccgatcgaga
     4261 acccggacgg tcgcgaccgc tacctggcga tgacgcaggc gttcgcccac cgacgaccga
     4321 cggccgacct cgacgatctg tcgcacaacg cggtctggcc ctgggggcgc ggcaatcact
     4381 acctgttcga cctgaaccgc gattggttca ccatggtcca gcccgagagc cggcggtccg
     4441 aggtgatcgc gtcgtggaac ccgcagctcg tggtcgacag ccacgagatg ggctcgaacg
     4501 acacctacct cttctcgccg gcgcggcatc cgttcaaccc ctacctgccg gaatcgcagg
     4561 cggcttgggg cgagcgcttc gccgccgacc aggcacgcgc gctcgaccgt cacggttatc
     4621 cgtactacac ccgcgagtgg aacgaggagt tcttccccgg ctacggctcg tcgtgggcgt
     4681 cgtatctcgg cgcggccggg atcctctacg agatgtcggg caccgagggc acgctggtca
     4741 agacgcgcga cggccgcctg cgcacctacc cgcaggccgt gcaccatcag acgacgagca
     4801 cgctggccaa tctgaccacg ctcgccgaca accgaagcga gatcctgcgc gacttcgtcg
     4861 ccgatcgccg caagacgatc gcgcgcgcgg gcaaggagtg gcccgcggcg tggatcctgc
     4921 cgcgtggtcg tcatccggcg cgcgtcgatc gtctggtcga cttgatgctg cggcagagca
     4981 tcgaggtcga gttcggcgac gcgcacgtgc cggggttgat cgactcgcgt accggtgaga
     5041 ccgcgccgtc cgacgcgttg accgaaccgt tctacgtcgt gcgcctcgat caaccggccg
     5101 cgccgctcgc acgccagctg ctcgacccgc acgtcccgat gaatgccgag ttctttcgcg
     5161 aggagcgcga gcatctcgag cgcggcaagg gcacgcggct gtacgaggtg acggcctggt
     5221 cgttgccgtt ggcctacgac gtcgatgcct attggaccga gacggcgccc gcgcgcgagg
     5281 gcttcaccgc cgcccggccg cagccggccg cgggagggat ggcgttcgtc gaagcaccgg
     5341 tcggctacct gatcgacggt ctctccgacg gctcgctgcc gctgctcgtc gagctgttgg
     5401 agcaggggat cacgccacgc gtcgccgaga agccgttccg cgtcgccggg cgcgattacc
     5461 cgcgaagcac gctgttcgtg ctcgccgagg gtcttccggc agacctcggc gagcgtctgg
     5521 ccgcggcggg cgagcgtcac ggcgtggtcg tgcgtgccgt gccgacgatc aaggccgagc
     5581 gagggcccga cctcggcggt ggccacttcc agccgctgcg ccagccgcgc gtcggcgtct
     5641 ggaccggctg gccggtgtcg ccgccggcct acggcgcgat ctggaacctg ctcgaccggg
     5701 acctcggcct acgcttcaac gcgctcgacg tggctcgcgc ccaatcgttc gatctgacgc
     5761 gctacaacgt actcgtcttt ccgccgactt tcgctggtca ggacgcctac gctcgcgtct
     5821 ttggcgaggc cgggctggcc cgcctgaagc ggtggatcga gggcgggggc acggcgatcg
     5881 gcgtcggcgc gggcgccgag ttcctggccg acgccggcca cggcctgacc gccacgcgac
     5941 tgcgacgcca ggcgctcgac gactacccac cggtcgtgct cggtcccgat gcagaacgcg
     6001 ccgaacgggc gggcgcattc cgcgccgtcg gcctgcgcgc gcccgagacg cgggacgacg
     6061 acgacgagga cgcgaagacc aagaccaaga ccaagagcaa gaccgaggcc gagaccgctg
     6121 ccgcggccgc cccgccgtac gacgtggcgc cggtgctcgg tccgggcgcg cgcccgttcg
     6181 cgcgcggaca cgagcagggc acgccggccg ccgagacgcc cgtcgatctg gcgcactgga
     6241 tggcgtcgtg gctcgcaccc gggagggaca agcccgacac ggaggagctc gagcgcgccg
     6301 accgacggct gcgtctgttc tcgccgcgcg gcgcctacct gcgtctcgag ctggacgaga
     6361 acgtctggct gacctggggg ctgcccggcg agctgccggc gctcgtgcgc gcgtcggaca
     6421 cgctggtcgc cgagccgccg gtccaagtcg cggcgcgctt cgcgccgatc gaccgcctgc
     6481 acctcggcgg cctgctgtgg cccgaggccg ccggccgtct cgcgctgacc gcgtacgcga
     6541 cgcgcgagag cctcggccgc ggacaggtgg tactgtttct gagcgagccc gagttccgcg
     6601 gattcacgct cggcacgcgc cgcctgctgg tcaacgcgat cctctacggg cccggtctcg
     6661 gcaccgagtg gtccaacccc tggtgagcga aaggaggttg acgatggact ggggcatgca
     6721 gaaccggctc agccggatcc tcgacccgcg gagcggccgg acggtgatgc tggcgatcga
     6781 ccacgggtac ttcctcggac cgacctccgg tctcgaggcg ccgggccgca cctgcgcgcc
     6841 gctgctgccg tacgccgact cgctgatgct gacgcgcggt acgctgcgcc agtgcatccc
     6901 cgcgaacagc aacgtgccga tcgtgctgcg cgtgtcgggc ggaacgagca tcctgcgcga
     6961 gctgtccaac gaggggctga ccgtcgcgat cgaggacgcg attcgactca acgcgtcggc
     7021 ggtcacgctg tccgtcttcg tcggcagcga aggcgagcgg acgacgctgc tcaacctggc
     7081 caagctcgtc gactacggcc agcaatacgg cgtgccggtg ctcgccgtca ccgcggtcgg
     7141 caaggagatg acgcgcgacg cgcgctacct cgggctggcc tgccgcatct cggccgagct
     7201 cggcgcgcat ttcgtcaaga cctactactg cgacgggttc gacgaggtcg tcgccgcgac
     7261 gccggtgccg atcgtgatcg ccggcggcaa gaagatcgcc gagcgcgagg cgatcgagct
     7321 ggcggcgaag gcggtccgcg ccggcgcgtc gggtgtcgac atggggcgca acatcttcca
     7381 gtccgactgc ccggtgggca tgatccgcgc ggtgcgatcg gtcgtgcacg atggcgcgag
     7441 cgccacgcac gcgttcgaga tctatcgcga acagaaggaa gccgccggtg cgggcagcgg
     7501 tctactacag caataacgac ctccggctcg aggagcgccc gacaccggtc gtcggtccgg
     7561 gcgagctgct gatgcgcgtc gaggcgagcg gcatctgcgg caccgacctg atcgagtggt
     7621 atcgcaagcc gaaggcgccg atcgtgctcg gtcacgaggt cgccggtacc gtcgtcgaaa
     7681 tcggcgccgg cgtcgaccgc ttcgtcgacg gcgatcgcat cgtcaccacg caccacgtgc
     7741 cctgcaacag ctgccgcttc tgcctgacgg accgccacag cgtctgcccg acgctgcacg
     7801 ccaccacgtt cgaccccggc gggttcgccg agttcgtgcg catcccggcg atcaacgtcg
     7861 agcggggaac gttccacctg ccggacaccg tttcgttcga gcaaggctcg ttcgtcgagc
     7921 cgctggcctg ctgcatccgc gcgcagcgtc tggcgcgggt cgcaccgggc gacagcgtgg
     7981 ccgtgctcgg cgccggcgtc gccggcatcc tgatgatcaa gctggcccgc gcgctcggcg
     8041 ccggacgcgt catcgcgacg gacacgagcg acgcgcgcgc cggtctcgcg gtcgccgccg
     8101 gtgccgacgt ggcgctgtcg gccgacgacc ccgagtgcat cgaccgggcg atcgcctcga
     8161 actacgcgcg ccgcatcgat cgcgtgctgg tc
//
LOCUS       DASPGN010000190         8290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_30882, whole genome shotgun sequence.
ACCESSION   DASPGN010000190 DASPGN010000000
VERSION     DASPGN010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8290
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30882"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VD788_05430"
     CDS             <1..187
                     /locus_tag="VD788_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75742.1"
                     /translation="SLQGTLVKRKVYLVRIDSELGKSMELEGFLSTDGSTIKGSWLNF
                     ELAGAEGSTGQWSATKR"
     gene            184..1431
                     /locus_tag="VD788_05435"
     CDS             184..1431
                     /locus_tag="VD788_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV75743.1"
                     /translation="MTRRLPYELFLALRYLRFHRGRTFLSVITLISIAGVAVGTAALI
                     IALSLMSGFVEDVRQRIHSGSAHLTVMSAVGGNVFEGAELLERRLEEIPGVYAAGTVL
                     YTHAMLTRDELDKPAFCELEGIDPNSHARVTLERGETDPFPVLAAPASSGRAGIVLGQ
                     QLARRLGAIPGDTIRVFVPKATLAPWGPMPVSHVFEVVGTYSSRHFQQDLQRAYVNID
                     DARSLLRQPGLTSWVEVRLDQLRRLQQMKTALRGELGPDWLVIDLIEQNQDLIKALNT
                     EKLVLFLAIGLIVVVAALNIVSTLILMVQDKIKEIGTLSAMGATPRGIARVFVFQGLV
                     IGVIGTAVGLIVGTVAAYVLDAYELIQLDPDVYYLTHVPLTTRPTDLVFVGLAALLVS
                     LLATIYPAWKAAKLHPVEAIRYE"
     gene            1424..2167
                     /locus_tag="VD788_05440"
     CDS             1424..2167
                     /locus_tag="VD788_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV75744.1"
                     /translation="MNEPLLVAEALDKAYGVGGARIDVLRGLSLAVRPGEAVAIVGES
                     GVGKSTLLHLLGGLDRPDAGRMSFAGLDVFSADTERMAEYRNRSVGFVFQFHHLLPEF
                     SAVENVAMPFRIGRRAGEIDAPAREMLERLGLGSRLTHRPSELSGGEQQRVAIARALV
                     GGPRLVLADEPTGNLDPTTGRRVFELLRELQRERGFALVLASHNERLAVECDRVLRLS
                     DGSLNEMARAETRLYFNGFGTEGSSDAML"
     gene            2202..4628
                     /locus_tag="VD788_05445"
     CDS             2202..4628
                     /locus_tag="VD788_05445"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HXV75745.1"
                     /translation="MFEKFTEKAKRILFLARYEASQHGSKQIGTEHILLGLLKEGEDS
                     TRELFSRANVSMDLLQAELEQRGPAREKLSTSVEIPFSDDTKRALQFAEEEAERLMHP
                     NIGTEHILLGLLRLEDSTAGRMLAERGMRLYAVREDVVNIHKRRSLPKKKKETPFLNE
                     FSRDLSEMAERRVFDPLIGREAELERVVQVLSRRRKNNPVLLGEPGVGKTAIIEGLAT
                     RIADGEVPPSLQGKRILALDLSLVVAGTKYRGQFEERLKGIITELTSSDDVIIFIDEI
                     HSLIGAGSAEGSLDAANILKPTLSRGEVQCVGATTPRDYHKHIEKDRALVRRFQPINI
                     KPPSEQETVAILLGVKERYERFHGVRFSEDSIRAAVFQSNRFITDRFLPDKAIDVLDE
                     AGARVKLGKTTSYADIKRVERELRRAVDGMKGALARKDFDEAVAFHDEEVTLRRQHED
                     LKQRYEVECNKILDVEQADVEEVISRWTGIPIQSVAEEEAEKLIHMEGYLHERIVGQE
                     AAISALARAIRRSRAGLNNPNRPIGSFIFLGPTGVGKTEVARTLAEFLFDSERALVRF
                     DMSEYMEKHAIAKMIGSPPGYVGHEEGGQLTERIKRKPYSVILLDEIEKAHPDILNIL
                     LQVLEDGVITDAYGQAVDFKNTIVIMTSNIGSSQVVRSGSKLGFKTDSREQAFKDRRD
                     LVMAEVKRTLSPEFINRIDELIVFDALSEEQLQQIAGIMIQRLNLGLADRGIQISVTA
                     EVGKWLVDTTCQDRSFGARPLRRAIQRHIEDALSEALIKGRFQDKGVIEVYLESNRLE
                     FRAAMEATST"
     gene            4767..7397
                     /gene="bamA"
                     /locus_tag="VD788_05450"
     CDS             4767..7397
                     /gene="bamA"
                     /locus_tag="VD788_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXV75746.1"
                     /translation="MTVRLGAWASTAPRARVAASYLLLSCFSFVSPVSAEPGTIEQII
                     PQGLTRMQERAFLHALGLEVGEPYTEAKVLRQFKALWDGGWFSDIVFETETGPAGGVV
                     LVIKVEERPVLSTVTYEENSVATRTAIEDRLRERNVTLKIGRPIDLGQVYYAETAIRD
                     LLAEKGHLNAQVEAKINKVTETSRAVHFLITSGGKTRIKKIEFTGNAVFKDGKLADQL
                     QLTSERKWYWPWSAKSLYHPVKWDQDAGNIRQLYLNNGYLDVEIRAPIVEIVQQGKER
                     GRAAEEQQPDSAADAADEMAKAAAAETELPVKEQRRLQNKRRKAEKKRRKQEQKAANV
                     KQQVYLTVPVIEGQQYTLGEVNISGNDELPVELVRALIPLREGEILRNDLLDGGIDRI
                     TRVYEDRGRLYANVVRQIERREGETVADVEVVIDEDKPYYVGRIEFVGNTSTRDNVLR
                     REVLLDEGDLFSRTALDVSARKLNQLGYWQAIQEPIVEPIEGENRVDVTIPGQEAGRN
                     EIQVGGGYSGVDGPFFNGVYSTRNFLGRGQTVSLALQVGGRSSRYQLSFQEPWLFNKP
                     YTFGFSIFRTGTDYGSSLTSQSTGFSVLLGKRVGRYSNVNLNYRWQNVSSTTIPLGST
                     IQPRTITTETTISSVTPIYRFSTINNPYRPTRGRDFNASFQLAGGPLGGDASFLKPIL
                     TVTNYTKALGKTFFAMHLQAGVIREFGGGSTLSGSNVQGVPRFERFWLGGDTLGPRVF
                     ETRSITPLRYVRLDGNNNIIDVTDDVVGQPADDFADNGAGIPVLIEAGGDRFYLFQTE
                     LVFPVNEQIEVATFFDLGDALFEDQSLGFETARASAGIELRFHLPIFPVPLRLIYGIP
                     VRKFERDRTSNFTFSIGRSF"
     gene            7524..8063
                     /locus_tag="VD788_05455"
     CDS             7524..8063
                     /locus_tag="VD788_05455"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXV75747.1"
                     /translation="MQRPIGMLATIAALIVLAGLGSSTAAQGSFKFGVFDPQRVSEET
                     AEGKRIQAKLEQLRDKSQQEIASEEGKIAELQQQLNQQSLSLSPEKRTQMEIDIQKRL
                     LQLNSRKDTMTRAFQLEIAGEEAQFNEKLRVVVGQFAQAEGFSVILEVGAVAFASPSV
                     DVTTAVIDRFNTMFPAVNE"
     gene            8079..>8290
                     /locus_tag="VD788_05460"
     CDS             8079..>8290
                     /locus_tag="VD788_05460"
                     /inference="COORDINATES: protein motif:HMM:NF016494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HXV75748.1"
                     /translation="MSRGFRLEELARCVGGTVVGDPTVVLHGVATLDAATPDQISFLI
                     NPRYRRLAQTTRAGAIIAKPGAGVAGR"
BASE COUNT         1499 a         2599 c         2837 g         1355 t
ORIGIN      
        1 gagcctgcag ggaacgctgg tcaagcgcaa agtctacctg gtccgcatcg actcggagct
       61 cggcaagtcg atggagctcg aaggatttct ctcgacggat ggatcgacga tcaagggcag
      121 ctggctcaac ttcgagctgg ccggggcgga agggtcgacg ggacagtggt cggcgacgaa
      181 acgatgaccc gtcgtctgcc ctacgagctg ttcctcgcgc tgcgctacct gcgattccac
      241 cgcggccgta ccttcctgtc cgtcatcacc ctgatttcga tcgccggcgt agcggtcggc
      301 actgccgcgc tgatcatcgc gctgtcgctg atgagtggct tcgtcgagga cgtgcggcag
      361 cggatccaca gcggcagcgc tcacctgacg gtgatgagcg cggtgggtgg caacgtgttc
      421 gagggagccg agttgctcga acgacggctg gaggagattc ccggcgtcta cgccgcgggg
      481 accgtgctct acacccacgc gatgttgacc cgcgacgagc tggacaagcc agcgttctgc
      541 gagctcgagg ggatcgatcc gaactcgcac gcccgcgtca cgctcgagcg gggggaaacc
      601 gacccgtttc cggtgcttgc ggcgccagca agcagcgggc gggccgggat cgtcctcgga
      661 cagcagttgg cgcgccgact gggggcgatt ccgggcgaca cgatacgggt cttcgtcccg
      721 aaggccacgc tcgctccgtg ggggccgatg cccgtgtccc acgttttcga agtggtcggc
      781 acctacagtt cccggcattt ccagcaggac ctgcagcggg cctacgtcaa catcgacgac
      841 gcgcggtcgt tgctgcgtca gccggggctg acgtcttggg tcgaggtgcg tctcgatcag
      901 ctgcgccgcc tgcagcagat gaagaccgcg ctgcgcggcg aactgggtcc cgactggctg
      961 gtcatcgacc tgatcgagca gaaccaagac ctgatcaagg cgctcaatac ggagaagctg
     1021 gtgttgttcc tcgcgatcgg gctgatcgtg gtcgtcgccg cgctgaacat cgtctcgacc
     1081 ctgattctca tggtccagga caagatcaag gagatcggca cgctgtcggc gatgggcgcg
     1141 acgccccgcg gcatcgcgcg ggtcttcgtc ttccagggtc tggtgatcgg cgtgatcggg
     1201 accgccgtcg ggttgatcgt cgggaccgtg gcggcctatg tgctcgacgc ctacgagttg
     1261 atccagctcg acccggacgt ctactacctg acccacgtgc cgctgaccac gcgaccgacg
     1321 gacctggtgt tcgtcggcct ggcggcgctc ctcgtctcgc tgctggcaac gatctacccg
     1381 gcgtggaagg ccgccaagct gcatccggtg gaggcgattc gctatgaatg agcctctctt
     1441 ggtggcggaa gcgctcgata aagcgtacgg ggtagggggt gcccggatcg acgtgctgcg
     1501 ggggctgagc ctcgccgtcc ggccgggcga ggccgtggcg atcgtgggcg aatccggggt
     1561 cggcaaatcg acgctgctgc acttgctcgg cgggctcgac cggccggacg cgggccgcat
     1621 gtcgttcgcc ggtctcgacg ttttctcggc ggataccgag cggatggccg agtaccgcaa
     1681 ccgttcggtc ggcttcgtct tccagttcca ccacctgctg cccgagttct ccgcggtcga
     1741 aaacgtggcg atgccgttcc gcatcggccg ccgcgcggga gagatcgacg cgccggcgcg
     1801 cgagatgctc gagcgactgg gactcggctc gcggttgacc caccggccgt ccgagctctc
     1861 gggcggcgag cagcagcggg tggcgatcgc gcgggcgctg gtgggcggcc cccggctggt
     1921 gctggccgac gagccgacgg gaaacctcga tccgacgacc ggccggcgcg tcttcgagct
     1981 gctgagggag ctgcagcgcg agcgcgggtt cgccctcgtc ctcgcctcgc acaacgagcg
     2041 tctggcggtc gagtgcgatc gggtgttgcg tttgtcggac gggtcgctga acgaaatggc
     2101 gcgggccgaa acccgcttgt atttcaacgg ctttgggacc gaagggtcgt cggacgctat
     2161 gctatagtca cacgcgctcc gcgaggcgag gttcgttggc gatgttcgag aaattcaccg
     2221 agaaggccaa acggattctg ttcctggccc gctacgaggc cagccagcac ggctccaagc
     2281 agatcggaac cgagcacatc ctgctcggac tgctcaagga gggcgaggac tccacgcgag
     2341 agctgttcag ccgtgccaat gtctcgatgg acctgctgca ggccgagctc gagcagcgcg
     2401 gcccggcgcg cgaaaagctg tcgacctcgg tcgagatccc gttcagcgac gacaccaagc
     2461 gcgccctgca gttcgccgag gaagaagccg agcgtctgat gcacccgaac atcggaaccg
     2521 agcacatcct gctcggcttg ctgcggctcg aagattcgac cgccggccgg atgctggccg
     2581 agcgcgggat gcggctgtac gccgtgcgcg aggacgtcgt caacattcac aagcggcgct
     2641 cgctgccgaa aaagaagaag gagaccccct ttctcaacga gttttcgcgc gatctgtcgg
     2701 agatggccga gcgccgggtg ttcgacccgt tgatcggccg cgaggcggag ctcgagcgcg
     2761 tggtgcaggt gctgtcgcgc cggcgcaaga acaacccggt cctgctcggc gaaccgggcg
     2821 tcggcaagac cgcgatcatc gagggtctgg cgacgcggat cgcggacggt gaagtgccgc
     2881 cgtcgctgca gggcaagcgc atcctcgcgc tcgatctgtc gctggtcgtc gccggtacca
     2941 agtaccgcgg tcagttcgag gagcgactga aggggatcat caccgagctt accagctccg
     3001 acgacgtgat catcttcatc gacgagatcc actcgctgat cggcgccggc tcggccgagg
     3061 gctcgctcga cgcggccaac attctcaagc cgaccctgtc ccgcggcgag gtgcagtgcg
     3121 tcggcgcgac gacgccgcgg gactaccaca agcacatcga aaaggatcgg gcgctcgtcc
     3181 ggcggttcca gccgatcaac atcaagccgc cgtccgagca ggagacggtc gcgatcttgt
     3241 tgggggtcaa ggaacgctac gagcggtttc acggtgtccg cttctcggaa gattcgattc
     3301 gcgccgccgt cttccagtcg aaccgtttca tcaccgaccg gttcctgccg gacaaggcga
     3361 tcgacgtgct ggacgaggcg ggtgcgcggg tcaagctcgg caagacgacc tcgtacgccg
     3421 acatcaagcg ggtcgagcgg gagctgcgcc gagcggtcga cgggatgaag ggtgcgttgg
     3481 cccgcaagga cttcgacgag gcggtggcgt tccacgacga agaggtgacg ctccggcgcc
     3541 aacacgagga tctgaaacag cggtacgagg tcgagtgcaa caagatcctc gatgtcgagc
     3601 aggccgacgt cgaggaagtc atctcgcgct ggaccggcat cccgatccag tccgtcgccg
     3661 aggaagaggc cgaaaagctg atccacatgg aaggttacct ccacgagcgt atcgtcgggc
     3721 aggaggcggc gatctcggcg ctggcgcgcg cgatccgtcg ctcgcgggcc gggttgaaca
     3781 acccgaatcg gccgatcggt tcgttcatct tcctcggacc gacgggagtc ggcaagaccg
     3841 aggtcgcgcg aacgctggcc gagttcctct tcgattcgga gcgggcgctc gtccgcttcg
     3901 acatgtcgga gtacatggaa aagcacgcga tcgccaagat gatcggctcg ccgccggggt
     3961 acgtcggtca cgaggagggc ggccagctca cggaacggat caagcgcaag ccgtacagcg
     4021 tgattctgct cgacgagatc gagaaggcgc acccggacat cttgaacatt ctgctgcagg
     4081 tgctcgagga cggcgtgatc accgacgcct acggtcaggc ggtcgacttc aagaacacga
     4141 tcgtgatcat gacctccaat atcgggtcgt cgcaggtggt cagaagcggc agcaagctcg
     4201 gcttcaagac cgactcgcgc gagcaggcgt tcaaggaccg ccgcgatctg gtgatggcgg
     4261 aggtgaagcg cacgctcagt ccggagttca tcaaccgcat cgacgagttg atcgtgttcg
     4321 acgcgctgag cgaggagcag ttgcagcaga tcgccgggat catgatccag cgcctgaacc
     4381 tcggactggc cgaccgcgga atccagatct cggtcaccgc cgaggtcggc aagtggctgg
     4441 tcgacacgac ctgccaagat cgctccttcg gcgcgcgccc gctgcgccga gcgatccagc
     4501 ggcacatcga ggacgcactg tccgaagccc tgatcaaggg ccgcttccag gacaaggggg
     4561 tcatcgaggt ctatctcgag agcaaccgtc tcgagttccg cgcggcgatg gaagcgacgt
     4621 cgacgtgatt ctcccggcgt tggagacgcg cggtcccgtt cagcatgtag aatgacccgt
     4681 ccggccggag gtggggtgcg gtgcgtggcc ggcacctggt gactgctgaa ccgatgttgt
     4741 gcgccaagcc catccgccgg aggggcatga cggttcgcct cggggcgtgg gcgtcgaccg
     4801 ccccgcgagc acgcgtcgca gccagctacc tgctactttc gtgtttcagc ttcgtctcac
     4861 ccgtctccgc cgagcccggg acgatcgagc agatcatccc gcaggggctg acccgcatgc
     4921 aggagcgggc cttcctccac gccctggggc tcgaggtcgg cgagccgtac accgaggcca
     4981 aggtgctccg gcagttcaag gccctgtggg atggcggttg gttctcggac atcgtgttcg
     5041 agaccgagac cggccccgcc ggcggggtgg tgctcgtgat caaggtcgag gagcgaccgg
     5101 tcctgtcgac ggtgacctac gaggagaaca gcgtcgcgac gcgcaccgcg atcgaggacc
     5161 ggctgcgcga gcgcaacgtc acgctcaaga tcggccgtcc gatcgacctc ggacaggtct
     5221 actacgccga gaccgcgatt cgtgacctgc tggccgagaa gggccacctc aacgcgcagg
     5281 tcgaggcgaa gatcaacaag gtcaccgaga cgtcgcgcgc ggttcacttc ctgatcacgt
     5341 cgggcggcaa gacgcgaatc aagaagatcg agttcaccgg taacgccgtg ttcaaggacg
     5401 gaaagctcgc cgatcaactt cagctcacct ccgagcgcaa gtggtactgg ccgtggtcgg
     5461 caaagagctt gtaccacccg gtcaagtggg atcaggacgc cggcaacatc cggcagctct
     5521 acctcaacaa cggttacctc gacgtcgaga tccgtgcgcc gatcgtcgag atcgtgcagc
     5581 agggaaagga gcgcggccgg gccgcggagg agcagcagcc tgacagcgcg gccgacgccg
     5641 cggacgagat ggcgaaggcg gccgccgccg aaacggagct gccggtcaag gaacagcgtc
     5701 gcctgcagaa caagcgacgc aaggcggaga agaagcgacg caagcaggag cagaaggcgg
     5761 ccaacgtcaa gcagcaggtc tacctgacgg tcccggtgat cgaggggcag cagtacacct
     5821 tgggcgaggt gaacatctcg ggtaacgacg agctgccggt cgagctggtc cgcgcgctga
     5881 tcccgctgcg cgagggcgag attctgcgca acgatctgct cgacggaggc atcgatcgga
     5941 tcacgcgcgt ctacgaagat cgcggccggc tctacgccaa cgtcgtccgt cagatcgagc
     6001 ggcgggaggg cgagacagtc gcagacgtcg aggtggtgat cgacgaggac aagccgtact
     6061 acgtcggccg aatcgagttc gtcggcaaca cgtcgacccg cgacaacgtc ttgcgccgcg
     6121 aggtactgct ggacgagggc gatctgttca gtcgaacggc gctcgacgta tcggcgcgga
     6181 agctgaacca gctcgggtac tggcaggcga tccaggagcc gatcgtcgag ccgatcgagg
     6241 gcgagaaccg cgtcgacgtg acgatccccg gccaggaggc ggggcgcaac gagatccagg
     6301 tcggcggggg atacagcggc gtcgacgggc cgttcttcaa cggcgtctac tcgacgcgca
     6361 actttctcgg ccggggccag acggtgtcgc tcgcgctgca ggtgggtggg cgatcgagcc
     6421 gctaccagct gtcgttccag gagccgtggc tgttcaacaa gccctacacc ttcggcttca
     6481 gcatcttccg caccggcacc gactacggct cgagcttgac cagtcagagc acggggttca
     6541 gcgtgctgct cggcaagcga gtcgggcgtt acagcaacgt caacctgaac taccgttggc
     6601 aaaacgtctc gtcgacgacg atcccgctgg gcagcacgat ccagccgcgg acgatcacca
     6661 ccgagacgac gatctcgagc gtcacgccga tctaccgctt ctcgacgatc aacaacccgt
     6721 acaggccgac gcgtggacgc gacttcaacg ccagcttcca gctggccggc ggtccgctgg
     6781 gaggcgacgc ctcgttcctc aagccgatcc tcacggtaac caactacacg aaggcactgg
     6841 gcaagacctt cttcgccatg catctgcaag cgggagtgat ccgcgagttc ggcggcggta
     6901 gcacgctgag cgggtcgaac gtgcagggcg ttccgcggtt cgagcggttc tggctcggcg
     6961 gggacaccct cggtccccgc gtgttcgaga cccgcagcat cacgccgttg cgctatgtcc
     7021 ggttggacgg gaacaacaac atcatcgacg tgaccgacga cgtggtcggg caaccggcgg
     7081 acgacttcgc cgacaacggc gccggcatac cggtgttgat cgaagccggc ggcgaccgat
     7141 tctacttgtt tcagacggag ctggtttttc ccgtcaacga gcagatcgag gtggcgacgt
     7201 tcttcgacct cggcgacgca ctgttcgagg accagtcgtt gggattcgag acagcgcgcg
     7261 cgtcggccgg gatcgagctg cgcttccatt tgccgatctt tccggtgccg ttgcgtctga
     7321 tctacggtat cccggtacgc aagttcgagc gcgaccggac ctcgaatttc accttctcga
     7381 tcggtcgcag cttctgacgc gcccgcgccc aggtctggcc gcggtggcac gaatgctgct
     7441 atcgtggtcg tgttcccgtg tcgcgcggct cgggccaggt gcagcggggc ggcgtgggaa
     7501 cggcgcaaca ggagagtagc tcgatgcaga gaccgatcgg aatgttggcg acgatcgccg
     7561 cgttgatcgt gctggcgggg ctcggctcgt cgaccgcggc acaaggcagc ttcaagttcg
     7621 gagtcttcga tccgcaacgc gtttcggagg agaccgcgga gggcaagcgc atccaagcca
     7681 agctcgagca gcttcgcgac aagagccagc aagaaatcgc gagcgaggag ggcaagatcg
     7741 ccgagctgca gcagcagttg aaccagcagt ccctgtcgtt gtcgccggaa aagcggacgc
     7801 agatggagat cgacatccag aagcgactgc tgcagctgaa cagccgcaag gacacgatga
     7861 cccgcgcgtt ccaactcgag atcgccggcg aggaagcgca attcaacgag aaactgcggg
     7921 tcgtcgtcgg ccagttcgcg caggccgaag ggttctcggt catcctcgag gtcggtgcgg
     7981 tcgccttcgc ttccccgtcg gtcgacgtca cgaccgcggt gatcgatcgg ttcaacacga
     8041 tgttcccggc tgtgaacgag tagcggagaa gctgcgcgtt gtcccgcggg tttcgcttgg
     8101 aggaactggc ccgttgcgtc ggcggtaccg tcgtcggcga cccgaccgtc gtgctgcacg
     8161 gcgtcgcgac gctcgatgcg gcaacacccg accagatcag ctttctgatc aacccgcggt
     8221 accgccgcct ggcgcagacg acgcgcgccg gggcgatcat cgccaagccc ggcgcgggtg
     8281 tcgccgggcg
//
LOCUS       DASPGN010000191         8708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_382652, whole genome shotgun sequence.
ACCESSION   DASPGN010000191 DASPGN010000000
VERSION     DASPGN010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382652"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VD788_05465"
     CDS             complement(<1..440)
                     /locus_tag="VD788_05465"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXV75749.1"
                     /translation="MPTPVPTLRRAVSRWQVVGLSLNDVVGSGVYLLPAAAAAALGPW
                     SVFAVLVAGLAVVLLVLCFAEAASRFDQPGSAYLYAKHAFGDFVGLEVGWMTWLARLA
                     SVSSLSVGFAQALSYALPAAGHGWARTATITLPVLLLTALNVIGV"
     gene            complement(476..1909)
                     /locus_tag="VD788_05470"
     CDS             complement(476..1909)
                     /locus_tag="VD788_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXV75750.1"
                     /translation="MEQHDDDAHGPLYRRVADRVAGWISAGRLESGERIPSIRKLARQ
                     MSVSVTTVLEAYRLLEDRGLIEARPQSGYYVRRPAPPGPEPARTETAERPAPPDVADL
                     AFDVLPHVGNPDLVPLGAAAPSTEFLPVERLNRLLARAVRLHPDTSHSYGRVPGWREL
                     RVQIARRGLDSGCSFSPDDVITTGGAQQALSLALRSVARPGETVAVESPTYHGLLQAI
                     ETQGLRALEIATDPRNGVCLDELRAVVEKRPIAAVAIAPSFGNPLGHSMPIADRRRLV
                     EMLADAGVPLIEDDVYGELPYEGARPQACKAFDRAGGVLLCSSFSKTIAPGYRVGWIV
                     PGRYFDAVQRAKYAAVIATAIPQQMAIAEFLASGGFDRHLRRLRRTYRDLSQRTICAI
                     REHFPDGTRVTRPTGGHLLWIELAEQVDALRVQRQALALGISVLPGPIFSPSGRYRNC
                     LRVNCAVPWSDRIENALRTLGHLSARG"
     gene            2078..3076
                     /locus_tag="VD788_05475"
     CDS             2078..3076
                     /locus_tag="VD788_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXV75751.1"
                     /translation="MTTDTPERASRAALAGAFSAIYLVWGSTYLAIKIGIETLPPFGM
                     AGLRFATAGCTLYAVLRLAGVPRPRLSEWRGAAWIGVLMLAGGNGLVTRAEAVVPSWV
                     AAVIIATVPLWMTLLEAWPFRRARLGPRVLIGLALGLGGVVTLVAPSGDRLTGVDPVG
                     AVLLLVAALSWSTGSLLSRSVALPGHPMMAASAQMIAGGAALLLWGTISGEWAGFDPA
                     AVSARSLAALSYLVTFGSMLALGCYVWLLRVTSAAAVSTYAFVNPLVALVLGWAALDE
                     SVAPRALVATLLIVSGVAFLQGSRAMARPALRRLFLRARVTPGGTSGRLARPGSTP"
     gene            3073..4029
                     /locus_tag="VD788_05480"
     CDS             3073..4029
                     /locus_tag="VD788_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXV75752.1"
                     /translation="MKRRERALRVLSIGGVLTLGALAGAVATADDEERRSKHEKGSVR
                     IVIVDDDGTHHEESFEFDADHPQAFLGVELDEGPDGGARVGRVVADSAAERAGLREGD
                     RIVAFDGRTIDRPWDLTREVLRARPGDVIEIEIDRDGRSETVDVELGERTHGAGRFVF
                     GFDDLDEQIERLHESLDEFDFDFGSSDEFEHGRSWRRSRPVLGVELVSVTPELRRHLG
                     AGDDEGVLVGKVLPGTPAERAGIAVGDLIVAVDGTVIDGPHALRAALREGAGRTIDVE
                     VVREGNRVHLSAGLPEVEQEDASHPGRPRARPATHTAAERRA"
     gene            complement(4043..5581)
                     /locus_tag="VD788_05485"
     CDS             complement(4043..5581)
                     /locus_tag="VD788_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HXV75753.1"
                     /translation="MRSFPRLSVSATLALIGAASLVDAREGATGALVQYWRGGFGSMT
                     GNRLEWTHLRLELEERDGSLSGSGILLAGPQLPSPVRLRAYGRMRATRIELTFDDRDS
                     RVACEGHGGSEFIEGECRRHGGRVDLRLVRVEPEVRADSLLGVYDLDGGHRVQIGRDA
                     LGLLMIDLRTGVVRSLFHKGGETYVAGPSLSVGHPAQWRLAFERDSGGAVTAVRIVDP
                     SGASRRAVRRAGWAEEDYSVLNERDGVMLRGTLTLPARDDAPLPAIVWVHGSGPVTRH
                     AAQFFPHYFADLGFAVLAVDKRGVGGSAGEYALPDGSSDNVRHLLRSADDVAWAIKTL
                     KRDPRIDPTRIGLVGVSQAGWVMPAAAALETCAFTITLSGGATELSREAFYSALTREE
                     LADASLPSIDELLPRVRSMLPTDLDWTPTFARQPCPGLWLYGMKDRVNPSQLSIELLD
                     WVRAFHDKDFTIVAFPDGNHALLDVRIGGLAGHHVADRFVPDLFVTIERWLGQLWLAS
                     EPPG"
     gene            5839..8676
                     /locus_tag="VD788_05490"
     CDS             5839..8676
                     /locus_tag="VD788_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75754.1"
                     /translation="MRGRVSRIASTAALAWWISGAAIAQTGPSLTLEPASGPAGSIVV
                     ASGTGFDAGSCGVELFLDSTGGSSLGTAELVGGAFSREIEIPAGTTEGAHEVIAQGLG
                     LVDSSCSMLTGEEGRASFEVLAAVTGPFVSDAVTPYVQTIDLGDLPIAPGWQPGDPIQ
                     VGPGETTDGPQALLSGDPTKTAPAVFSPFFKVRERLRYGLPDFDRLERSGPTRQMLRA
                     AMMTHAKGEPLLNFDGIAATGVIPASPAGDVGPEHFIQTVNAAFAVYDKQGNLLAGPA
                     SLGALWSGSGGACEAGNDGDPDVRYDAMDDRWVISSIVAFDTQCLAISRDGDPVSGGW
                     YLYEFPTGDVKNDYPRLAVWPGAYFVGTQRDYPNGGSDVWAFDRAAMLGGLPAVSIRF
                     NDSGLYLLPADIDGGSVPAAPAPGLFVRIGDGAQIGGDDRLEVFEFLADFTNPPASTF
                     GSVGVLAPAPFDRDLCGLGLNTPCVPQPDGAPALDAFTALPMARLQFRVFDDHDALVF
                     NHTVDVDGADHAGVRWYELRRAGGSWSIYQQGTHAPDAGEAGLADDPQRWMASAAMDK
                     NGNLALGYSAAGAALDPGVRFGGRYAADPPSETPREERSFVEGGGSQTHPSGRWGEFS
                     SMTIDPVDDCTFWYTAQYYAEDSEAGWSTRIGTFQLDDVPPEISCPTDAIAECSATGG
                     TPSDDEQLAEFFGGVTASDDCSIETQIANDAPGLFPLGQTDVTFTATDGETNAAECVA
                     VVEVEDTTMPEIVAPPDIEDFECTSPEGASPELGTPQVEDICDADPTVDNDAPSIFPI
                     GTTNVTWTATDQSDNVGSDTQSVEVVDTTPPSLAVWSTPSVLWSPNHKMIDIVVSVEV
                     SDVCDSEVGVKLVSIDSNEPPNGNGDGNTQPDFDHADFGTDDRRFRLRAERKGNGDGR
                     VYTIVYAATDDSGNETLASTTVTVPHDQSGD"
BASE COUNT         1336 a         2977 c         3096 g         1299 t
ORIGIN      
        1 acgccgatca cgttgagcgc ggtgaggagc agcaccggaa gcgtgatcgt cgcggtcctg
       61 gcccagccgt ggccggcggc cggcagcgcg tagctcagcg cctgggcgaa cccgaccgag
      121 agcgacgaga ccgaagcgag ccgtgccagc caggtcatcc agccgacttc gaggccgacg
      181 aagtcaccga aggcgtgctt cgcgtagagg tacgcgcttc ccggctggtc gaagcggctc
      241 gccgcctcgg cgaagcacag cacgagcagc acgaccgcca ggccggcgac gaggacggcg
      301 aagacgctcc aaggcccgag ggcggccgcg gcggcagccg gcagcaggta gaccccgctg
      361 ccgacgacgt cgttcaacga caggccgacg acttgccagc gcgaaaccgc ccgtctcagc
      421 gtcggaacgg gtgtgggcat cggaggccga atctacgggc gcgacggcgt ccgcgtcagc
      481 cccgcgcgct caggtgaccg agcgtgcgca acgcgttttc gatccgatcg gaccacggta
      541 ccgcgcagtt gacgcgcagg cagtttcgat atcgaccgct cggtgagaag atcggtcccg
      601 gcaatacgct gatcccgaga gccagcgcct ggcgctgcac ccgcaacgcg tcgacctgct
      661 cggccagctc gatccacagc aggtgcccgc ccgtcggacg cgtgacgcgg gtcccgtccg
      721 gaaaatgctc gcgaatcgcg cagatcgtcc gctggctcag gtcgcgatag gtgcgtcgca
      781 gccgtctgag gtgccggtcg aacccgccgc tcgcgaggaa ctcggcgatc gccatctgct
      841 gtgggatcgc cgtcgcgatg acggcggcgt acttcgcgcg ctggaccgcg tcgaagtagc
      901 gcccggggac gatccagccg acccggtagc cgggggcgat cgtcttgctg aaggacgagc
      961 aaaggagcac gccgccggcc cggtcgaacg ccttgcaggc ctgcggacgc gcgccttcgt
     1021 aaggcagctc gccgtagacg tcgtcctcga tcaacggcac gccggcgtcg gccagcatct
     1081 cgaccagcct gcgacgatcg gcgatcggca tcgagtgccc gagcgggttg ccgaagctcg
     1141 gggcgatcgc gacggcggcg atcgggcgct tctcgaccac ggcgcggagc tcgtcgagac
     1201 agacgccgtt gcgcggatcg gtcgcgatct cgagcgcgcg caggccctgt gtctcgatcg
     1261 cctgcagcag tccgtggtag gtcggtgact cgaccgccac ggtctctccc ggacgggcca
     1321 ccgagcgcaa tgcgagcgac agcgcctgtt gggcgccgcc cgtcgtgatc acgtcgtccg
     1381 gagagaacga gcagccggag tcgagaccgc gacgggcgat ctggacccgc agttcccgcc
     1441 agcccggcac cctgccgtac gagtggctgg tgtcggggtg caggcgcacc gcgcgtgcga
     1501 ggagccgatt cagtcgttcg acggggagga actcggtgct cggtgcggcg gcgccgagcg
     1561 ggaccaggtc ggggttgccg acgtgcggca gcacgtcgaa cgcgagatcg gccacgtcgg
     1621 gcggcgccgg tcgctcggcc gtttcggtcc tcgccggttc cggtccgggc ggggccggac
     1681 ggcgtacgta gtaccccgac tggggtcgag cctcgatcag cccgcggtcc tcgagcagac
     1741 ggtaggcttc gagcacggtc gtgacgctga ccgacatctg acgggcgagc ttgcgaatcg
     1801 acgggatccg ctccccggac tcgagcctgc ccgccgagat ccagccggcg acccggtcgg
     1861 cgacacgccg gtagaggggt ccgtgggcat cgtcgtcgtg ttgttccacc agaccggcca
     1921 ttcgtccccc ccgcgcgtac agcccgtcga ctcacctggg agtacagctg aagaaacctg
     1981 aactgtactg gttgcaattt tattcaccta catctgttct gtcgagccat ttcacgccag
     2041 aatcgaaggc aacgaacgaa gagtggcgga gagggcgatg acgaccgaca cacccgaacg
     2101 cgcgtcccga gcggcgctcg ccggcgcgtt ctcggcgatt tacctggtct ggggctcgac
     2161 ctacctggcg atcaagatcg ggatcgagac gttgccgccg ttcgggatgg cgggtctgcg
     2221 cttcgccacc gcgggttgca cgctctacgc cgtgcttcgc cttgccggcg tcccgcggcc
     2281 gcgcctttcg gaatggcgtg gagcggcttg gatcggggtt ctgatgctcg ccggcggaaa
     2341 cggtctcgtc actcgagccg aggcggtcgt gccgtcgtgg gtcgcggccg tgatcatcgc
     2401 cacggttccc ctgtggatga ccctgctcga ggcgtggcca ttccgtcgag cccgcctcgg
     2461 tccgcgggtg ctgatcgggt tggccctcgg tttggggggc gtggtcacgc tggtcgcgcc
     2521 gagcggagat cgcttgacgg gagtcgatcc cgtcggcgcg gtcttgctcc tcgtcgctgc
     2581 cctgtcgtgg tcgaccgggt cgctgttgtc gcggtcggtc gcgttacccg ggcacccgat
     2641 gatggccgct tcggcccaga tgatcgcggg aggcgcggcg ctgttgttgt ggggaacgat
     2701 ctcgggggag tgggccgggt tcgacccggc cgcggtctcg gcccgctcac tggccgcgct
     2761 gtcgtacctc gtcaccttcg gttcgatgct cgcgctcggc tgctacgtgt ggctgttgcg
     2821 ggtcacctcg gctgccgccg tctcgaccta cgccttcgtc aacccgctgg tcgcgctcgt
     2881 gctcggttgg gccgcgctcg acgagtcggt cgcgccgcgc gccctcgtcg ccacgttgct
     2941 gatcgtctcg ggggtcgcat tcctgcaagg ttcccgggcg atggcacgcc ccgctctccg
     3001 acgacttttt ctccgagcgc gcgtaacacc gggtggaacg agcgggcgtc tcgcccgccc
     3061 cgggagcacg ccatgaagag acgagagcga gcattgcggg ttctgtcgat cggcggggtc
     3121 ttgacactgg gcgcgttggc cggcgcggtc gcgacggccg acgacgagga gcgccggtcg
     3181 aagcacgaga agggcagcgt gcgcatcgtg atcgtcgacg acgatggcac gcaccacgag
     3241 gaatcgttcg aattcgatgc cgaccacccg caggcattcc tcggcgtgga gctcgacgag
     3301 gggccggacg gcggggcgcg cgtcggacgc gtcgtcgccg acagcgcggc cgagcgcgcg
     3361 ggtttgcgcg agggcgaccg gatcgtcgcg ttcgacggcc ggacgatcga ccgaccctgg
     3421 gacctgaccc gcgaggtgct ccgcgcgcgt cccggcgacg tgatcgagat cgagatcgac
     3481 cgcgacggac ggtccgaaac cgtcgacgtc gagctcggcg agcggaccca cggcgcgggg
     3541 cggttcgtct tcggtttcga cgacctggac gagcagatcg agcgtctcca cgaatcgctc
     3601 gatgagttcg acttcgactt cgggtcgtcc gacgagttcg agcacgggcg ctcgtggcga
     3661 cgatcacgcc cggtgctcgg cgtcgagctc gtctcggtga cgcccgagtt gcggcggcac
     3721 ctcggggccg gcgacgacga gggcgtcttg gtcggcaagg tgctgccggg gacgccggcg
     3781 gagcgggcgg gcatcgccgt cggtgacctg atcgtcgccg tcgacggtac ggtgatcgac
     3841 gggccgcacg cgctgcgggc cgcgctgcgc gaaggggcgg gccggacgat cgacgtcgag
     3901 gtcgtccgcg aggggaaccg cgtgcacctg tcggcgggcc tccccgaagt cgagcaggag
     3961 gacgcgagcc acccggggcg gccgcgagcg cggccggcga cccacaccgc ggcggagcgg
     4021 cgcgcctgac cgtgcccgag gctcagccgg gaggctccga cgccagccac agctgtccca
     4081 gccagcgctc gatcgtcacg aacagatcgg ggacgaatcg atcggccacg tggtgcccgg
     4141 ccagccctcc gatgcgcacg tcgagtagcg catggttgcc gtccgggaat gcgacgatgg
     4201 tgaagtcctt gtcgtggaag gcccgcaccc agtccagcag ctcgatgctg agttgggacg
     4261 gattgacgcg gtccttcatc ccgtatagcc acagaccggg gcacggctgt cgggcgaacg
     4321 tcggggtcca gtcgagatcg gtcggcagca tcgagcgcac ccgcggcagg agctcgtcga
     4381 tcgagggcaa cgacgcgtcg gccagctctt cccgggtcag cgcgctgtag aacgcctcac
     4441 gactgagctc cgtcgcgcca ccgctcagcg tgatggtgaa ggcgcaggtc tcgagcgcag
     4501 ccgccgcggg catgacccag ccggcttggc tcacgcccac caggccgatc cgcgtcgggt
     4561 cgatgcgagg gtcgcgcttg agcgtcttga tcgcccaggc gacgtcatcc gcgctcctca
     4621 gcaggtgccg cacgttgtcg gaactcccgt cgggcaaggc gtattctccc gccgatccgc
     4681 cgacgccgcg tttgtcgacc gcgaggacgg cgaagcccag atccgcgaag tagtggggaa
     4741 agaactgcgc cgcgtgccgg gtcacgggac ccgaaccgtg gacccaaacg atcgcgggca
     4801 gcggcgcgtc gtctcgcgcg ggcagcgtca acgtaccgcg cagcatcacc ccgtcgcgct
     4861 cgttgagcac gctgtagtcc tcctccgccc agcccgcacg acggacggct cggcgcgacg
     4921 ctccggacgg gtcgacgatt cggaccgccg tgacggcgcc gccggaatca cgctcgaacg
     4981 ccaatcgcca ctgtgcgggg tgcccgaccg acaggctcgg gccggcgacg taggtctcgc
     5041 cacccttgtg gaacagcgag cgcacgacac cggttcgaag gtcgatcatc agcaagccca
     5101 gcgcgtcgcg cccgatctga acgcggtggc cgccgtcgag atcgtagacg ccgagcagcg
     5161 agtccgcccg gacttccggt tcgacacgaa ccagccggag atcgacccgc ccgccgtggc
     5221 gacggcactc tccctcgatg aactccgagc cgccgtggcc ttcgcacgcg acgcgcgagt
     5281 cgcgatcgtc gaaggtcagt tcgatacggg tcgcgcgcat ccggccgtac gctcgcagcc
     5341 ggacgggaga cggcagctgc ggtccggcca gcaggatgcc gctgccgctg aggctgccgt
     5401 cgcgctcttc gagctcgagc cgaagatggg tccactcgag ccggttcccg gtcatcgacc
     5461 cgaacccgcc ccgccagtat tggacgagcg cgccggtcgc cccctcgcgc gcatcgacga
     5521 gggatgcggc cccgatcagc gccagggtcg cggagacgga gagtcggggg aaggagcgca
     5581 tcggccgaaa ggcaaaaggt accgcaatcc ggggcccaag gcctggtttt cggctggttg
     5641 ccgcaccggc cgggtagctg caaccgattc gtgacgtttt gacgcgacaa cgaccccgac
     5701 tatgcgataa acttgcggca ctcgaacaac ccgggtgatc cgggggatgg gtcaagacaa
     5761 ccgcggcgcg gcggtctcac ggcgacgggc cgaggcgtcg cacagcgttc gaccggttcg
     5821 gcggtccagg ggcaaatcat gagaggacgt gtgagtcgga tcgcgagcac ggctgcgctc
     5881 gcgtggtgga tctcgggggc ggccatcgcc cagaccggac ccagcctgac gctcgaaccg
     5941 gcgagcggtc cggcagggag catcgtcgtc gcgagtggca cggggtttga cgccggctcg
     6001 tgcggtgtcg agttgtttct cgactcgacc ggtggcagtt cgctcggcac cgccgaactg
     6061 gtgggcggcg cgttctcgcg cgagatcgag attccggccg ggacgaccga gggtgcgcac
     6121 gaagtcatcg cgcaaggtct cggtctggtc gatagctcgt gctcgatgtt gaccggcgag
     6181 gaagggcgcg cgagcttcga ggtgctggcg gccgtgaccg gcccgttcgt cagtgacgcg
     6241 gtcacgccct acgtgcagac gatcgacctc ggcgacctgc cgatcgcccc gggatggcag
     6301 ccgggcgatc cgatccaggt cgggcccggg gagacgacgg acggtccgca ggcgttgctg
     6361 tccggggatc cgacgaagac ggcgcccgcc gtcttctcac cgttcttcaa agtgcgggaa
     6421 cggctgcgct acggccttcc cgatttcgat cggctcgagc gcagcgggcc gacccgacag
     6481 atgttgcgcg ccgcgatgat gacgcacgcc aagggcgagc cgctgctgaa cttcgacggg
     6541 atcgcggcca cgggcgtgat cccggccagc cccgctggcg acgtcgggcc ggagcatttc
     6601 atccagaccg tcaacgccgc gttcgcggtc tacgacaagc agggcaacct gctcgccggt
     6661 cccgcgagtc tcggcgcgct gtggagcggc agcggcggtg cctgcgaggc cggcaacgac
     6721 ggcgacccgg acgtgcgtta cgatgcgatg gacgatcgct gggtcatcag ctcgatcgtg
     6781 gcgttcgaca cgcagtgcct cgcgatctcg cgggatggcg atccggtctc cggcggctgg
     6841 tatctctacg agttcccgac cggcgacgtg aagaacgact acccgcggct cgcggtctgg
     6901 cccggcgcct acttcgtcgg cacgcagcgt gactatccca acggtggttc cgacgtctgg
     6961 gctttcgacc gtgcggcgat gctcggcggc ctgcctgccg tctcgatccg attcaacgac
     7021 tccgggctct acctgctacc ggccgacatc gacggcggct cggtccccgc ggcgccggcg
     7081 cccggcctct tcgtgcgcat cggcgacggg gcccagatcg gcggcgacga ccggctcgag
     7141 gtcttcgagt ttctcgcgga cttcacgaac ccgcccgcat cgacgttcgg ctcggtcggc
     7201 gtgctggcgc ccgcgccctt cgatcgcgac ctgtgcggtc tcgggttgaa cacgccctgc
     7261 gtcccacagc ccgacggcgc gccggcactc gacgcgttca ccgcgctgcc gatggcccgc
     7321 ctacagtttc gcgtgttcga cgaccacgac gcgctcgtgt tcaaccacac cgtcgacgtc
     7381 gacggtgccg accacgccgg cgtgcgctgg tacgagctgc gccgcgccgg cggctcgtgg
     7441 tcgatttacc agcaggggac ccatgcaccg gacgccggcg aggcggggct ggccgacgac
     7501 ccgcagcgct ggatggccag tgcggcgatg gacaagaacg ggaatctcgc gctcggctac
     7561 agcgccgccg gcgccgcgct cgatcccggt gttcgcttcg gcggccgcta cgccgccgac
     7621 ccgccgagcg agacgccgcg cgaggaaagg tcgttcgtcg aaggcggcgg atcgcagacc
     7681 catccgagcg ggcgctgggg cgagttcagc tcgatgacga tcgatccggt cgacgattgc
     7741 accttctggt acacagcgca gtactacgcc gaagacagcg aggcgggttg gagcacgcgc
     7801 atcggcacct tccagctcga cgacgtgccg cccgagatca gctgcccgac cgacgcgatt
     7861 gccgagtgtt cggcgaccgg tggcacaccg tccgacgacg agcagctcgc cgagttcttc
     7921 ggcggcgtga ccgccagcga cgactgctcg atcgagaccc agatcgcgaa cgacgctccg
     7981 gggctgttcc cgctgggcca gaccgacgtc accttcaccg caaccgacgg cgagaccaac
     8041 gcggccgagt gcgtcgcggt ggtcgaggtc gaggacacga cgatgcccga gatcgtcgcg
     8101 ccgccggaca tcgaggactt cgagtgcacc tcccccgagg gcgcctcgcc cgagctcggc
     8161 acgccgcagg tcgaggacat ctgcgacgcc gatccgacgg tggacaacga cgccccatcg
     8221 atcttcccga tcgggacgac gaacgtcacc tggaccgcga cggaccagag cgacaacgtc
     8281 ggcagcgaca cgcagtcggt ggaggtcgtc gacacgacgc cgccgtcgct cgcggtgtgg
     8341 tccacgccgt ctgttctgtg gtcgccgaac cacaagatga tcgacatcgt tgtttccgtc
     8401 gaggtgagcg acgtttgtga cagcgaggtc ggcgtcaaat tggtctcgat cgacagcaac
     8461 gagccgccca acgggaacgg cgatggcaac acgcagcccg atttcgacca cgccgatttc
     8521 ggcaccgacg atcgacggtt ccgcctgcgc gccgagcgca aggggaacgg cgacggcagg
     8581 gtctacacca tcgtctacgc cgccaccgac gactccggta acgagacgtt ggcctcgacc
     8641 accgtcacgg tgccgcacga tcagagcggc gactgagcgg tcgccgcaac ggaccgtcgg
     8701 gccccgcc
//
LOCUS       DASPGN010000192         7179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_500454, whole genome shotgun sequence.
ACCESSION   DASPGN010000192 DASPGN010000000
VERSION     DASPGN010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500454"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VD788_05495"
     CDS             <1..463
                     /locus_tag="VD788_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75755.1"
                     /translation="AAPVAVAAARRPAGNSAAWGVGVVVVLVAVVLAVATHDFAPQPP
                     APAREVRKPTPQVVASLAAVKTEIQHNEQGQMVEIAAGDPRAILDAFCRAQRRGTVDP
                     VAIEPAGEHWTGLYRQNGQLFGIAIRRDPERDLWVAGNAVDGLEGDPISSR"
     gene            601..1650
                     /locus_tag="VD788_05500"
     CDS             601..1650
                     /locus_tag="VD788_05500"
                     /inference="COORDINATES: protein motif:HMM:NF017617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HXV75756.1"
                     /translation="MKNSTVRRRPAAAIQPLSPAERKKAFELFGRYDPADPESVLKFG
                     TSVQAQVSKFQDRVMSHVQAKDNRRVIDLLDRLLGRLRALEMTPNGRPSILRKLRAFR
                     SRLSWTEQCMADYHELGQTLERQIDELNDARGQLLAEVAVFEKLYEQNLVLLRNVGLG
                     LRVLDWTVNGLREKLLPRVRRKLRGSHDPEWTLELQDVTDRIDRLERRARDLALTREI
                     CVQSARQIRLSQHDRQMLAERILDAVLNAIPLWKTQVSIALIQSRQRETMKLYQRIMR
                     ATDQALDRNRSILDQGRLDASRGTRGSLRGTRRGPLAVEPDVRPDRAPAPAAPSATVP
                     PAPMKTMKPAGGLEH"
     gene            complement(1592..2194)
                     /locus_tag="VD788_05505"
     CDS             complement(1592..2194)
                     /locus_tag="VD788_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75757.1"
                     /translation="MVDRRIRRTVPVAAAVVAALAISAPPATAARGAAELSLDRTATA
                     ALVEAALSGPYRRELEGFGPVTIELGSPRSLAFRDGGIETTIPVRIAGSSLAAVIEAR
                     FVPAIDRLTGQIRLESEYARPAGAWPLPLDFAALFEPVGLPQRLSWTIELERAVGLRA
                     DCFVQGLEIGRERLVLLLALELSAPGRPPVSSSSSAPAAP"
     gene            complement(2203..3720)
                     /locus_tag="VD788_05510"
     CDS             complement(2203..3720)
                     /locus_tag="VD788_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75758.1"
                     /translation="MVRRLVLALAWAALAGCGSDYLPPDLPTTGAPAGPLGQVLDLDV
                     VFPNLTVGQQPRPGVAFEMRVEIERTGFGEFAARVAYGSARLATAIVPIEDLSDGRTS
                     ITVTPDRWASGRLGPLRIDDATFEMMLDGSAEEEGWRVAGRALGSRSLDNGSFVGFRR
                     HRFLVAGTDFLSTSGRLAEVVLVRETEVRVRHGLGLVSSDPVLGAGNSAVFVVNRLTF
                     DNLQRLDPERDFATSWQRGVGAGSNPQYALRLSDERGYLSRYEPPYNDVAVFSPADGT
                     ILGSIPLETWAENPDGTPRPTTIVRAAGAVFVALQDVDRSFMHYAEGKLAVIDPDTDR
                     VIGTILLGGRNPLGIDLWVDAGGREKLYVALAGIFPGTRPQELSGGVVVVDALNRVVD
                     RMALDDDVAGGNIGAVAIVRESLGYATVSDERFVNRVVAFDPRSGSVLRTVVESDELI
                     PTIAADGHGVLAVPDRRFFEPQTCLYRVPIDEGASETRIHCAAMELPPFSLVALD"
     gene            complement(3720..5741)
                     /locus_tag="VD788_05515"
     CDS             complement(3720..5741)
                     /locus_tag="VD788_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV75759.1"
                     /translation="MRRPIAPLCCLLLAVFRLPAGDGGKAEADDNPAEPVIDEKVEVR
                     EEAPRDADVAAFATRIDRGEFARRGADLGDVLRRVPGARVRDYGGLGSYATMSLRAST
                     SEQVTVLVDGVPQNRALGGPVDLSSIPATQIDRVTVYRGFAPAAYGLGGLGGVVDVRT
                     RDAGGPLRAQVDLLAGELGTTRLSGGASLRTGAHGRLRVGTEWLKSDGDFHYLDSGET
                     LFQADDDVIRRRLNNGVDQASILVRQSSENVGRGELDVGLRVQHRERGLPGLGTFTSE
                     SARLEERVEAADLSWSRRYATRVESLDLQADLFHQRIGFADPAGEIGLRSESRTTELA
                     GGGGTATTRARFGSHRALMRVDMRHERAAVTDEARVPSDLGGAQRNLVGVTIEDLFGT
                     GPLTVAPSVRWQYREDDFTAGSSGAVEPAADDVRDSSWSGKVGLAWALGPAASIRGSV
                     GRFYRVPSLVELFGDRGSLIANPTLVPESGVTAELGYARGHRSGRLPWSVELVGFARR
                     AKNLIRFVSVSQGTAVARNLADVESYGVEGSLSARPVRGLTVDASATVQRAEDLSPGY
                     TSGKALPYQPELLAYLGAGYARGRLQARWEATYTGANATDSLDTPELRLPERIIHDVN
                     LELLLPGGFALGVDLRNIFDRRTLDLLRHPLPGRTLFVHLGWRTHPAAD"
     gene            complement(5738..6961)
                     /locus_tag="VD788_05520"
     CDS             complement(5738..6961)
                     /locus_tag="VD788_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75760.1"
                     /translation="MRRTNGLACAALVAAAGLAAAGERSRPIDCLPDRLAAWQAAEPD
                     PETRFGALSLPGIVLGPPGDSVAYQGSVTVASLGFGGRAVYAFDDLSIEDRPGPDFVV
                     FENGFFALPLPAAEEDEFLIFAEPGLVEVSLDGVDWRSFPYDAQALVDSIALGGSIDR
                     GLYLRMVGLAGLTPTFTGNWTVPDDPAVFDPAGTAGVSGAGGDAFDLADVGLPSARFV
                     RITDAGTGAGFAGSAEGFDLDALVVLHGRPGPAPGVDQDHDGLSDAEEAVRYGSDPAL
                     ADSDGDGVDDGREVASCRDPASTSTAPWIVLEPALWLAGSTCTEARWTFVGSSVSYDL
                     VRADGDAIGAVAGQVDLGSTTCLADSPLGVRWSCDAETPLPGDWFGYLVRVGGATDWG
                     RSSALEPRGTAQECP"
     gene            complement(6963..>7179)
                     /locus_tag="VD788_05525"
     CDS             complement(6963..>7179)
                     /locus_tag="VD788_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75761.1"
                     /translation="LPLPGAVTVVDLADLPLRRYSLDTIGLMLWSDGFGGPVPALLAD
                     VEFHGLFVHPDLLPSADAILRLPPRRR"
BASE COUNT         1145 a         2603 c         2482 g          949 t
ORIGIN      
        1 ggccgctccc gtcgccgtcg ctgcggcacg ccgaccggcc ggcaactccg ccgcctgggg
       61 tgtcggcgtt gtcgtcgtgc tggtcgcggt cgtgctcgcg gtcgcgaccc acgacttcgc
      121 gcctcaacca cccgcaccgg cgcgcgaggt ccgcaaaccc acgccgcagg tcgtcgcctc
      181 gctggcggcc gtcaagaccg agattcagca caacgagcag ggtcagatgg tcgagatcgc
      241 cgccggcgat ccgcgagcga tcctcgacgc cttctgccgc gcgcagaggc gtgggaccgt
      301 cgacccggtc gcgatcgaac cggctggcga gcactggacc gggctgtacc gccagaatgg
      361 gcagttgttc ggcatcgcca tccggcgcga tcccgagcgc gacctgtggg tcgccggcaa
      421 cgccgtcgac ggtctcgagg gcgatccgat ttccagccgc taggctcgac cgcgcgaccg
      481 ggccggtcga tcgatccgtc gcggggaaac gcgacacgga tcacgatttc gtcgccttcg
      541 ggtggacaaa tcggggatca gaacggatca tgacagggta gccgctcaaa cgggtcctgc
      601 atgaaaaact cgacggtccg gcgccgcccg gctgccgcga tccaaccgct ctcgcccgcc
      661 gaacgcaaga aggcgttcga gctattcggt cggtacgacc cggccgaccc ggagagcgtg
      721 ctgaagttcg gcacctccgt ccaggctcag gtctccaagt tccaggatcg ggtgatgtcg
      781 cacgtccagg ccaaggacaa ccggcgagtg atcgacctgc tcgatcgcct gctcggacgg
      841 ttgagggcgc tcgagatgac gcccaacggt cgaccgtcga ttctgcgcaa gctgcgcgcc
      901 tttcgcagcc ggctctcctg gaccgagcag tgcatggccg actaccacga gctcggacag
      961 acgctcgagc gccagatcga cgagttgaac gacgcacgcg gccagctcct ggcggaggtc
     1021 gccgtgttcg agaagctgta cgagcagaac ctggttctgc tgcgcaacgt cggtctcggg
     1081 ttgcgcgtcc tcgattggac cgtcaacggc ctgcgcgaga agctcctccc gcgtgtgcgc
     1141 cggaagctgc gggggtcgca cgacccggaa tggacgctcg agctgcagga cgtgaccgat
     1201 cggatcgatc gtctcgagcg ccgcgcgcgc gatctggcgc tgacgcgcga gatctgcgtg
     1261 cagagcgctc gccagatccg cctgtcccaa cacgaccgcc agatgctcgc ggagcggatc
     1321 ctcgacgcgg tgctcaacgc gattccgctg tggaagaccc aggtcagcat cgcgctgata
     1381 cagtcgcgac aacgagagac gatgaagctc taccagcgga tcatgcgtgc caccgatcag
     1441 gcgctcgacc ggaaccgatc gatcctggat cagggtcgtc tcgatgcgtc gcgcggcacg
     1501 cgcggctcgc tccgcggcac gcgccgcggc ccgctggcgg tcgaacccga cgttcgcccc
     1561 gaccgcgcgc ccgcgccggc cgccccgtcg gctacggtgc cgccggcgcc gatgaagacg
     1621 atgaaaccgg cgggcggcct ggagcactga gctcgagagc gagcagcaac accagtcgct
     1681 cacggccgat ctccagcccc tggacgaagc aatcggcgcg caagccgacg gcgcgctcga
     1741 gctcgatcgt ccacgacagc cgttgcggca gccccaccgg ctcgaacagc gcagcgaagt
     1801 cgagcggcag cggccacgcg ccggccggac gcgcgtactc gctttcgagc cggatctgcc
     1861 cggtcagccg gtcgatcgcc ggcacgaaac gcgcctcgat caccgcggcg agcgacgacc
     1921 cggcgatccg caccggaatc gtcgtctcga tgcccccgtc gcggaaggcg agcgagcgcg
     1981 gcgaccccag ttcgatcgtc accgggccga aaccctcgag ctcgcgtcga tagggtccgg
     2041 agagcgccgc ctcgaccagc gccgcggtcg ccgtccgatc caggctcagc tcggccgccc
     2101 ctcgcgcggc ggtggcgggt ggggccgaga tcgcgagcgc cgccaccacg gcggcggcga
     2161 cgggcaccgt tcggcggatt cgacggtcga ccatgacgct cctcagtcga gcgcgacgag
     2221 cgagaacggc ggcagctcca tcgccgcgca gtggatccgc gtctcgcttg cgccctcgtc
     2281 gatcggtacg cggtacaagc acgtttgcgg ttcgaagaaa cgtcggtcgg gcacggccag
     2341 caccccgtgt ccgtcggcgg cgatcgtggg aatcagctcg tcgctttcga ccacggtgcg
     2401 caacaccgag ccgctgcgcg ggtcgaacgc gacgacccgg ttgacgaacc gctcgtccga
     2461 gacggtggcg tagcccaacg actcccgcac gatggcgacg gcgccgatgt tgccgccggc
     2521 gacgtcgtca tcgagcgcca ttcgatcgac gacgcggttc aacgcatcga cgaccaccac
     2581 gccgccggac agctcctggg gccgagtccc ggggaagatc ccggccagcg cgacgtagag
     2641 cttctcgcgt ccgccggcgt cgacccacag gtcgatcccg agcggattgc gaccaccgag
     2701 caggatcgtg ccgatcaccc gatcggtatc agggtcgatg acggccagct tgccctcggc
     2761 gtagtgcatg aacgatcgat cgacgtcctg tagcgcgacg aacacggcac cggcggcccg
     2821 cacgatcgtc gtcggacgcg gcgtaccgtc cgggttctcg gcccacgtct cgagcgggat
     2881 gctgccgaga atcgtcccgt ccgctggcga gaagaccgcc acgtcgttgt acggcggctc
     2941 gtaacgcgag aggtagccgc gttcgtcgga caggcgcaat gcgtactgtg gattcgaccc
     3001 ggctccgacc ccgcgttgcc agctcgtggc gaagtcgcgt tccggatcga gtcgctggag
     3061 attgtcgaac gtcagccggt tgacgacgaa cacggcggag ttgccggcac cgagcacggg
     3121 atccgagctg acgagcccga gcccgtgacg gacacggacc tcggtttccc gcacgagcac
     3181 gacctccgcc aggcgtcccg aggtcgacag gaaatcggtc cccgcgacga ggaagcgatg
     3241 gcggcggaac ccgacgaagc tcccgttgtc gaggcttcga ctgccgagcg cgcgtcccgc
     3301 gacccgccac ccttcttcct cggcagaccc gtcgagcatc atctcgaacg tcgcgtcgtc
     3361 gatacgcagc gggccgaggc ggccgctcgc ccagcgatcg ggagtgacgg tgatcgatgt
     3421 gcgtccgtcc gagagatcct cgatcgggac gatcgccgtc gccagccgcg ccgacccgta
     3481 ggcgacgcgc gcggcgaact cgccgaaccc tgtccgctcg atctcgacgc gcatctcgaa
     3541 cgcgacgccc ggccgcggtt gttggccgac cgtgagattc ggaaagacga cgtcgagatc
     3601 gagcacctgt cccaacggtc ccgccggcgc tcccgtcgtc ggcaggtcgg gaggcaggta
     3661 gtccgaccca cagccggcaa gcgcggccca ggccaaagcg agcacgagcc gccggaccat
     3721 cagtccgccg ccgggtgagt acgccagccc aggtggacga acagcgtccg gcccggcaac
     3781 gggtggcgca gcagatcgag cgtccggcgg tcgaagatgt tgcgcagatc gacgccgagc
     3841 gcgaagccgc cgggaaggag cagctcgagg ttcacgtcgt ggatgatccg ctccggcaag
     3901 cgcagctccg gcgtgtcgag ggagtcggtg gcgttcgctc cggtgtaggt cgcctcccag
     3961 cgggcctgca ggcgtccgcg tgcgtagccc gcgccgaggt aggccagcag ctccggctgg
     4021 tacggcagcg cctttccgga ggtgtatccg ggggagaggt cctccgcgcg ctgcaccgtg
     4081 gcgctggcgt cgacggtgag tccgcggacc ggccgggccg acagcgagcc ttcgacaccg
     4141 tagctctcga catcggcgag gttgcgcgcg accgccgtgc cctgactgac cgagacgaag
     4201 cggatcaggt tcttcgcgcg gcgcgcgaag ccgacgagct cgaccgacca cggaagccgg
     4261 ccggagcggt ggcctcgcgc gtagcccagc tcggccgtca ctccgctctc gggcacgagc
     4321 gtcggattgg cgatcagcga gccgcgatcg ccgaacagct cgacgaggct cggcacccgg
     4381 tagaatcgac cgaccgaacc gcggatcgag gcggccggcc cgagcgccca ggccagcccg
     4441 accttccccg accacgacga atctcggacg tcgtccgctg ccggctcgac cgccccgctc
     4501 gaaccggcgg tgaagtcgtc ctcgcggtac tgccagcgga cggacggcgc gacggtcaac
     4561 gggcctgtgc cgaacaggtc ctcgatcgtc acgccgacga ggttccgctg tgcgccgccc
     4621 agatcggacg gaacgcgcgc ctcgtcggtg accgcggccc gttcgtgccg catgtcgacg
     4681 cgcatcagcg cgcggtgcga accgaatcgt gcgcgcgtcg tggccgtgcc gccgccgccc
     4741 gcgagttcgg tcgtgcgcga ctccgagcgc agcccgatct cgccggcggg atcggcgaac
     4801 ccgatccgtt ggtggaacag atccgcctgc aggtcgagcg actcgacccg ggtcgcgtag
     4861 cggcggctcc acgacaggtc ggcggcctcc acgcgctctt ccagccgggc ggattccgag
     4921 gtgaaggtgc cgagaccggg tagcccgcgc tcgcggtgct gcacgcgcaa accgacgtcg
     4981 agctcgccgc ggccgacgtt ctcgctcgac tgtcgtacga ggatggacgc ctgatcgacc
     5041 ccgttgttca ggcgcctgcg gatcacgtcg tcgtcggctt ggaacaacgt ctcgccggag
     5101 tcgaggtagt ggaagtcccc gtcactcttg agccactcgg tgccgacccg cagccttccg
     5161 tgcgcgccgg tacgcaggct cgcgccgccc gacagtcggg tggtgcccag ctctcccgcg
     5221 agcaggtcga cctgcgcccg cagcggccca ccggcgtcgc gggtgcgcac gtcgacgact
     5281 ccaccgagtc cacccaggcc atacgccgcg ggggcgaagc cgcgatagac cgtcacccgg
     5341 tcgatctgcg tcgcgggaat cgaggacagg tcgaccggac cgcccagcgc ccggttctgc
     5401 gggactccgt cgaccagcac cgtcacctgc tcggaggtcg aagcacgcaa cgacatcgtg
     5461 gcgtagcttc ccagcccgcc gtagtcacga acccgcgccc cgggtacgcg tctgagcacg
     5521 tcgcccaggt ccgcgccgcg ccgcgcgaac tcgccgcggt cgatccgcgt tgcgaacgcc
     5581 gcgacgtccg catcccgtgg cgcttcctcg cggacctcga ccttctcgtc gatgaccggc
     5641 tcggccgggt tgtcgtcggc ttccgccttg ccgccgtcac ccgccggcaa ccggaagacc
     5701 gccagcagca gacagcaaag cggcgcgatc ggtcgtctca cgggcactcc tgcgcggtgc
     5761 cgcgcggctc gagcgccgac gagcggcccc agtccgtcgc ccccccgact cgcaccaggt
     5821 agccgaacca gtcgccgggc aacggcgtct ccgcgtcgca cgaccagcgc acgccgagcg
     5881 gcgagtcggc caggcaggtc gtcgagccga ggtcgacctg gccggcgacg gcgccgatcg
     5941 cgtcgccgtc cgcgcgaacc aggtcgtagc tcacgctgct gccgacgaag gtccagcggg
     6001 cctcggtgca ggtcgatccg gcgagccaca acgcgggctc gagcacgatc cacggcgcgg
     6061 tcgacgtcga ggcaggatcg cgacaggacg cgacctcgcg gccgtcgtcc acgccgtcgc
     6121 cgtcactgtc ggccagcgcc gggtcgctgc cgtaccgtac ggcttcctcg gcgtcgctca
     6181 ggccgtcgtg gtcctggtcg acacccggcg cgggccccgg ccggccgtgc agcacgacca
     6241 acgcgtcgag gtcgaagccc tcggcggatc ccgcgaatcc cgcgcccgtc ccggcgtccg
     6301 tgatccggac gaaccgcgcc gacggcagac cgacgtcggc gaggtcgaat gcgtcaccgc
     6361 cagcgccgga gacgccggcg gtgccggcgg gatcgaacac cgccgggtcg tcgggcaccg
     6421 tccagttgcc ggtgaaggtc ggggtcagcc cggcgagccc gaccatccgc aggtagagcc
     6481 cgcggtcgat cgagccaccg agcgcgatcg agtcgacgag cgcctgcgca tcgtacggga
     6541 acgatcgcca gtcgacgccg tcgagcgaca cctcgaccaa gccgggctcg gcgaagatga
     6601 ggaactcgtc ctcctccgca gccggcagcg gcaacgcgaa gaagccgttc tcgaaaacga
     6661 cgaagtccgg ccccggacga tcctcgatcg acagatcgtc gaacgcgtag accgcccgcc
     6721 cgccgaaacc gagcgaggcg acggtgaccg acccctggta ggcgaccgag tcgcccggcg
     6781 gcccgagcac gatccccggg agcgacaggg cgccgaagcg cgtttccggg tcgggctcgg
     6841 cggcctgcca cgcggccaac cggtctggca ggcaatcgat cggtcgcgag cgctcgcccg
     6901 cagcggcaag cccggccgcg gcaaccagcg cggcgcaggc gagcccgttc gtccgcctca
     6961 cgtcagcgtc tccgcggcgg cagccgcagg atcgcgtcgg ccgacggcag caggtccggg
     7021 tggacgaaca gaccgtgaaa ctcgacgtcg gccaacagcg ccggcaccgg gccgccgaag
     7081 ccgtcgctcc acagcatcag gccgatcgtg tcgaggctgt agcgtcgcag cggtaggtcg
     7141 gcgaggtcga cgacggtgac ggcgccgggc agcgggagg
//
LOCUS       DASPGN010000193         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_148839, whole genome shotgun sequence.
ACCESSION   DASPGN010000193 DASPGN010000000
VERSION     DASPGN010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148839"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..2721
                     /locus_tag="VD788_05530"
     CDS             262..2721
                     /locus_tag="VD788_05530"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV75762.1"
                     /translation="MPAESNPALPGRRYLLPPERLRWRCDPAHFNFVTTADMQRCPIQ
                     IIGQTRAMEALELGLSMRSEGYNIFVTGEVGSGRSTVVKRMLEGMERGDRSPNDVVYV
                     HNFADADEPRRLVLPARQGRLLRESMEDLVDSLIRDLRGLFDSDEYRRQRGAIVDQTT
                     HEQKARLKEFEKRVQEHGFALVQVPMGPLTRPQPVPVVAGNPVDMDQLEHLVEQGQFK
                     KEDLDRLQERGSELSGEMEALGKSVRNADRDLRRKLAVLDRDLARPLVEEAAGDVAAS
                     FKADGLELYLDQVVEDVLANLDSFRDTGERALGEEATPSQTDGAPTRDRYRVNVVVDN
                     SKTKGQPIIWETAPTYRNLFGTIEKVRTPNGDWITDHMHIKGGSLMRANGGILVVDAL
                     DLLIEPGVWAALKRTLRNRSVEILSFDPLNIFAGVSLKPEAVPISVKVIMLGTRQIYS
                     LLYYHDEDFRTIFKVKSEFAMFTERSAEELDNYACFVYKKVKDDDLPPFRREAVAAVV
                     EHGVRLAGDLDKLTTRFTVIADLIREAGYWARRAGADSVDEGHVRQALRKRIERVSLF
                     EELLYERIVDGTVLIDFSGSKVGQINGLALLDQGDHTFAQPARITASTAVGRHGIIDI
                     DREAEMSGTLHTKGVLILSGFLRSRFAQNKPLALTASLTFEQSYGGIDGDSASSAELY
                     ALISSLSGVPLRQGIAVTGSVNQKGEIQPIGGVNRKIEGFFDLCRLVGMTGEQGVLIP
                     KRNERNLMLREEVVEAVREGRFHVWSVETIEQGLGVLTDVEPGQLGGDGRYPEGSIYR
                     RVDDRLARLAEEVRKYGNAAQ"
     gene            complement(2715..>3284)
                     /locus_tag="VD788_05535"
     CDS             complement(2715..>3284)
                     /locus_tag="VD788_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HXV75763.1"
                     /translation="VVVPIWRGGDPKDEILAAASDATGRLRATGLSVKLDDRDTMSPG
                     AKYHEWELAGVPLRIEIGPKDVAKGSVVCVRRTDRGKEFVSMEQLATRVPTLLAEIQR
                     QLFEAAVERRERATYPVDDFDDFKRKVEDPGGFLLAHWCGSAECEQQVKNSTRATLRC
                     VAFDQPDERGRCIVCGRPSARRAHFAKAY"
BASE COUNT          571 a         1081 c         1120 g          512 t
ORIGIN      
        1 tctcgccggc atcgatgccg taacgccacg ccgcgcctgc gaccattcgc tggtgacgcc
       61 ccaccaactc ggagaacgcg cgctggtcgc cgcggagcgt cgccgtgacg aggctgaggt
      121 cctggcgtcc cccgtcgtcc atcgaagccc cgttcgtctt gtgttacgcc cgcgccgccg
      181 ggtcggttcc cgcggaatcc gactccggat cgcggtagta taggctcgca gtcgacaacg
      241 agtacgattg aggacccgtc gatgcccgcg gaatcgaacc cagccctgcc cggccgccgc
      301 tacctgctcc caccggagcg actgcgctgg cgctgcgacc cggcacattt caacttcgtc
      361 accacggccg acatgcagcg gtgcccgatc cagatcatcg gccagacccg ggcgatggag
      421 gcgctcgagc tgggtctgag catgcgcagc gaggggtaca acatcttcgt cacgggtgag
      481 gtcggcagcg gccggtcgac cgtcgtcaag cgcatgctcg aggggatgga gcggggcgat
      541 cgatcaccga acgacgtggt ctacgtgcac aatttcgccg acgccgacga gccgcgccgg
      601 ttggtgttgc cggcaaggca gggccgcctg ctgcgcgagt cgatggaaga cctggtcgat
      661 tcgttgattc gcgacctgcg gggattgttc gactcggatg agtaccggcg gcaacgcgga
      721 gcgatcgtcg accagacgac ccacgagcag aaggcgcggc tcaaagagtt cgagaagcgg
      781 gtgcaggagc acggcttcgc gctggtccaa gtcccgatgg ggccgctcac ccgaccgcag
      841 cccgtgccgg tggtcgcggg caacccggtc gacatggacc agttggagca cctcgtcgag
      901 caagggcagt tcaagaagga agacctcgac cgcctgcagg agcgtggcag cgagctgtcg
      961 ggagaaatgg aggcgctcgg caagtcggtg cgcaacgccg atcgggatct gcgtcgcaag
     1021 ctggcggtgc tcgaccgcga cctggcccgg ccactggtcg aggaggcggc cggcgacgtc
     1081 gccgcttcgt tcaaggccga cgggctcgag ctctacctcg atcaggtggt cgaggacgtc
     1141 ctggccaacc tcgactcgtt ccgcgacacc ggcgagcgcg cgctgggtga ggaggcgacg
     1201 ccgtcccaaa ccgacggggc accgacgcgc gaccgttacc gggtcaacgt cgtggtcgac
     1261 aacagcaaga cgaaggggca gccgatcatc tgggagaccg cgccgaccta tcggaacctg
     1321 ttcggcacga tcgagaaggt gcgcacgccg aacggggact ggatcaccga ccacatgcac
     1381 atcaagggcg ggagcctgat gcgcgccaac ggcgggatcc tcgtcgtcga cgcgctcgat
     1441 ctgctgatcg agcccggtgt ctgggccgcg ctgaaacgga cgctgcgcaa tcgctcggtc
     1501 gagatcctgt cgttcgatcc gctgaacatc ttcgccgggg tgtcgctcaa gccggaggcg
     1561 gtgccgatca gcgtcaaggt gatcatgctc ggcacgcggc agatctacag cctgctgtat
     1621 taccacgacg aagacttccg cacgatcttc aaggtcaagt ccgagttcgc gatgttcacc
     1681 gagcgcagcg cggaagagct ggacaactac gcctgcttcg tttacaagaa agtcaaggac
     1741 gacgacctgc cgccgttccg ccgcgaggcg gtggccgcgg tcgtcgagca cggcgtgcgg
     1801 ttggcgggcg acctcgacaa gctgacgacg cgattcaccg tgatcgccga cctgatccgc
     1861 gaggcgggat actgggcgcg acgagcgggt gccgacagcg tcgacgaagg gcacgtgcgg
     1921 caggcgctcc ggaagcgaat cgagcgcgtc agcctgttcg aggagctgct gtacgaacgg
     1981 atcgtcgacg gcacggtgct gatcgacttc agcggcagca aggtcggaca gatcaacggt
     2041 ctggcattgc tcgatcaggg agaccacacc ttcgcccagc ccgctcgcat cacggcctcg
     2101 acggcggtcg gacgccacgg gatcatcgac atcgaccgcg aggcggagat gtccggcacg
     2161 ctccacacca agggagtgtt gattctctcc ggcttcctgc gctcgcgttt cgcgcaaaac
     2221 aaaccgctcg cgctgaccgc cagcttgacg ttcgagcaga gctacggcgg gatcgacggc
     2281 gactcagcct cgtcggccga gttgtacgcg ctgatctcgt cactctccgg tgtcccgctg
     2341 cgccagggca tcgccgtcac cgggtcggtc aaccagaagg gcgagatcca gccgatcggt
     2401 ggtgtcaacc gcaagatcga gggcttcttc gacctgtgtc gactggtcgg catgaccggc
     2461 gagcagggcg tgctgatccc gaaacgcaac gagcggaacc tgatgctgcg cgaggaggtg
     2521 gtcgaagccg tgcgcgaggg ccggttccac gtctggtcgg tcgaaacgat cgaacagggc
     2581 ctcggcgtgc tgaccgacgt cgagccgggg cagctgggcg gcgatgggcg ctatcccgag
     2641 gggtcgatct atcgcagggt cgacgatcgg ttggcccggc tcgccgagga ggtccgcaag
     2701 tacgggaacg ccgctcagta ggccttggcg aagtgcgcgc gccgcgcgga cggccggccg
     2761 caaacgatgc agcggccgcg ttcgtcgggt tggtcgaacg cgacacagcg cagcgtggcc
     2821 cgcgtcgagt tcttcacctg ctgttcgcac tcggccgagc cgcaccagtg cgccaagaga
     2881 aaccctcccg gatcctcgac tttccgcttg aagtcgtcga agtcgtcgac cgggtaggtc
     2941 gcccgctccc tgcgctcgac ggccgcctcg aatagctgcc gttggatctc ggcgagcaac
     3001 gtcggcacgc gcgtcgccag ctgttccatc gagacgaact ccttgccgcg gtcggtacgc
     3061 cggacgcaga cgaccgagcc tttcgcgaca tccttcgggc cgatctcgat ccgcagcgga
     3121 acgcccgcca gctcccactc gtggtacttg gcccccgggc tcatcgtgtc ccgatcgtcg
     3181 agcttgacgg aaagaccggt cgcgcgcagc cggccggtcg cgtcgctcgc cgcggcgagg
     3241 atctcgtcct tggggtcgcc gccgcgccag atcggcacca ccac
//
LOCUS       DASPGN010000194         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_267208, whole genome shotgun sequence.
ACCESSION   DASPGN010000194 DASPGN010000000
VERSION     DASPGN010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267208"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="VD788_05540"
     CDS             complement(<1..1684)
                     /locus_tag="VD788_05540"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXV75764.1"
                     /translation="MKVYDTSDIRNVALIGHGDSGKTSLASAMLFVAGAVKRLGKVDD
                     GTAVTDFDDEEINRKISLQTGVAHVEWKGKKVNIIDTPGYAAFLADTKVAMTVADAVF
                     LLVESVAGVQVITERVFGYTEQFAVPLVFVISRSDRENASFERTLAAIQQRFGRTAVP
                     IQLPIGEAQQFEGVVNLLSMKALRFAGDGSGKLTEGEVPAELADSAAESRAALVEIVA
                     ETDDVLMEAFFEAGDLSEEQLLVGLRRGIVERKVFPVISTAATRIIGIQPVLDAIAQL
                     IPAPGDRGPRPGKNPADDSDLTREVASDQPVSAFVFKTIADPFAGKLSMLRVMSGTLK
                     ADSPVINPRRDGHSERLGNLSIMQGKQTVPIGELRAGDIGVIAKLKETQTSDTLCDPN
                     RPIVYPTVTFAEPAISYAVEPKSKGDEEKISTALARMMEEDPVLRVRRDARSAELLVS
                     GTGQVHVEVTLAKMKRKFGVEAILHPPKVPYFETIKRKVSNVEGKHKKQSGGRGQFGV
                     CVVDVEPLPRGSGFEFVDKIFGGSIPQNYRPAVEKGIVEAAERGWLAGAPVID"
     gene            1920..2594
                     /locus_tag="VD788_05545"
     CDS             1920..2594
                     /locus_tag="VD788_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HXV75765.1"
                     /translation="MVVSCPACQTRYHYRDSPPRGGRARCGRCGNVVPLVEAPARVVV
                     RARVAPVAQPQPRAAAAAIGTRSATATATAALASPDLFSPTDGLGEFGVLASDFPVHQ
                     PEPTVVGPADPRSSDALAVVPTLAPRPRAGDNAFATGARRVPAPAPVRARRRSASAGA
                     VVQLLALVSFAAGLGAAGHFAEVYAWLPPIEIHPMVDPVRPVMVGGLIGVLLGWIVVR
                     WISRSR"
     gene            2573..>2949
                     /locus_tag="VD788_05550"
     CDS             2573..>2949
                     /locus_tag="VD788_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75766.1"
                     /translation="MDLTQPLIEQRRADTFGPEPDRCLDCGSPPAGSRPIELSLYPGR
                     WLRRCKRCGVRYALDHTGVWRLFHCLGCGLPLLTDDVSSGAARRCGDCRSASNGATPT
                     DGDVARATEREVRAALRAEWRFVG"
BASE COUNT          430 a         1028 c          991 g          500 t
ORIGIN      
        1 agtcgatgac gggcgcgccg gccagccaac cgcgctcggc cgcctcgacg atacccttct
       61 cgaccgccgg gcggtagttc tgcggaatcg agccgccgaa gatcttgtcg acgaactcga
      121 agccggagcc gcgcggcaac ggctcgacgt cgacgacgca gacgccgaat tggccgcggc
      181 cgccgctctg cttcttgtgc ttgccctcga cgttgctgac cttgcgtttg atcgtctcga
      241 agtaggggac cttcggcgga tggagaatcg cctcgacgcc gaacttgcgc ttcatcttgg
      301 ccagcgtcac ctcgacgtgg acctgccccg taccggagac cagcagctcc gccgagcggg
      361 catcacgacg cacgcgcagc acggggtctt cctccatcat ccgcgcgagc gcagtcgaga
      421 tcttctcctc gtcgcccttg gatttgggct cgacggcgta ggagatcgcc ggttcggcga
      481 aggtgaccgt cgggtagacg atcggccggt tcggatcgca cagcgtgtcc gacgtctgcg
      541 tttccttgag cttggcgatc acgccgatgt cgccggcgcg cagctcgccg atcggaaccg
      601 tctgcttccc ctgcatgatc gacagattgc cgagacgctc ggaatgcccg tcgcggcgag
      661 ggttgatcac cggagagtcc gccttgagcg ttccgctcat cacgcgcagc atcgacagct
      721 tgccggcgaa cgggtcggcg atcgtcttga acacgaaggc cgaaaccggc tggtcgctcg
      781 cgacctcgcg tgtgaggtcg gagtcgtccg ccgggttctt gcccggtcgc ggcccgcggt
      841 cgccgggggc ggggatcagc tgcgcgatgg cgtcgagcac cggctggatc ccgatgatgc
      901 gggtcgcggc ggtggagatc acgggaaaga ccttgcgctc gacgatgcct cgacgcagcc
      961 cgaccagcag ctgctcctcg ctgaggtcgc ccgcctcgaa gaacgcctcc atcagcacgt
     1021 cgtcggtctc ggcgacgatc tcgaccaggg cggcacggct ttccgcggcg ctgtcggcca
     1081 gctcggccgg gacctcgccc tcggtcagct tgcccgaacc gtcgccggcg aagcgcagcg
     1141 ccttcatcga cagcaggttg accacgccct cgaactgctg cgcctcgccg atcggcagct
     1201 ggatcggcac cgccgtccga ccgaagcgct gctggatcgc ggccagcgtt cgctcgaacg
     1261 acgcgttctc ccgatcgctg cggctgatca cgaaaaccag cggcacggcg aactgctcgg
     1321 tgtaaccgaa tacgcgctcg gtaatcacct gcaccccggc gaccgactcg accagcagga
     1381 acacggcatc ggcgacggtc atcgcgacct tggtgtcggc gagaaacgcc gcgtagcccg
     1441 gcgtgtcgat gatgttgacc ttcttccctt tccattccac gtgggcgacg ccggtctgca
     1501 gcgagatctt gcggttgatt tcctcgtcgt cgaagtcggt cacggccgtc ccgtcgtcga
     1561 ccttacccag gcgcttgact gctcccgcga cgaacaacat cgcgctggcc aggctggtct
     1621 tgcccgagtc gccgtgcccg atcagggcca cattccggat gtcggaggtg tcgtacacct
     1681 tcatcactct ctctcctttg aaggcgatgc cccgcgagga ctcgagatgt cgaaaagtga
     1741 cggaagaggc ggaatatacc acagcgtcag aaacgccctc aaacgctgca atcgcgggac
     1801 ttccccacat cgcgaccggt tacattcttg accgcggcag gcaccgaacc taggtttccc
     1861 tgggcgtggg tcgccggaca cgacaggtgt ccgaaaacga gacgattgcg aggtccgtca
     1921 tggtggtcag ttgtccggct tgtcaaacgc gatatcacta ccgcgactcg cccccgcgag
     1981 gcggtcgggc gcgctgcggt cgctgcggga acgtcgttcc gctggtcgag gcccccgcgc
     2041 gagtcgtcgt ccgggcccgc gtcgcaccgg tcgcgcaacc gcagccgcga gcggccgcgg
     2101 ccgccatcgg tacgcggtcc gcgaccgcga ccgcgaccgc cgcgctggcc tcgcccgacc
     2161 tgttctcccc gaccgacggc ctcggcgagt tcggcgtgct ggccagcgac ttcccggtgc
     2221 accagccgga accgacggtc gtcgggcccg ctgatccccg ctcctccgac gctctggccg
     2281 tcgtgccgac gctggcgcca cggccgcggg ccggagacaa tgcgttcgcc accggcgcca
     2341 gacgtgtgcc ggcgccggcg ccggtgcgtg cccgacgacg ctcggcctcg gccggcgcgg
     2401 tcgttcagtt gctggcactg gtgtcgttcg cggccggcct gggtgcagcc ggccacttcg
     2461 ccgaggtata cgcgtggctg ccgccgatcg agatccaccc gatggtcgat ccggtccgac
     2521 cggtgatggt cggcgggctg atcggcgtgt tgctgggatg gatcgtggtg cgatggatct
     2581 cacgcagccg ctgatcgagc agcgccgcgc cgacacgttc gggcccgagc cggaccgctg
     2641 cctcgactgt ggcagcccgc ccgccggttc ccggccgatc gagctgtcgc tctatccggg
     2701 tcgatggcta agacgttgca agcgctgtgg cgtgcggtat gccctcgacc acaccggcgt
     2761 ctggcggctg ttccattgcc tcggttgcgg cctgccgttg ctgaccgacg acgtgtcgag
     2821 cggtgcagcg cgacgctgcg gcgattgtag gtcggcgagc aacggggcga cgccgaccga
     2881 cggcgacgtc gcgcgggcga ccgagcgcga ggtgcgcgcg gcactgcgcg ccgagtggcg
     2941 cttcgtcgg
//
LOCUS       DASPGN010000195         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1672246, whole genome shotgun sequence.
ACCESSION   DASPGN010000195 DASPGN010000000
VERSION     DASPGN010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672246"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VD788_05555"
     CDS             <1..468
                     /locus_tag="VD788_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV75767.1"
                     /translation="LDATTGDLEPAVRQAIDRLRPALESTGVTVELEVRPPVPAAVFD
                     REALHQILQNLLDNSSKFNRDATDRTVRVHLAAPAGRPTLEVIDRGSGVAREVRRKLF
                     RPFSHHPDPGAPAGLGLGLAIVRALAAAQGAEVSFRDRPGGGAVFSVRFDRGS"
     gene            545..2152
                     /gene="serA"
                     /locus_tag="VD788_05560"
     CDS             545..2152
                     /gene="serA"
                     /locus_tag="VD788_05560"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXV75768.1"
                     /translation="MSSQNPNPPARVLIADNLSENGVELLRGSPDIEVTVKIGMTPDE
                     LGVTIGDYDALVVRSATKVTAEVLERPGRLRAIGRAGTGVDNIDLEAATRAGVVVMNT
                     PGGNSVAAAELTLAHLIALARHLVQANHELRRGRWERKKYVGSEIEGKTIGIVGLGRI
                     GREVARRAKGLRMEVVGYDPYVSTDASAGFGVRGVTLDELLAQADFVTLHLPLSDNTR
                     HLIDASKLAKMKRGARLINCARGGLVDEDALFDALESGQLAGAALDVFEAEPPADRRL
                     VDHPLVVATPHLGASTREAQERVGSEIAEKIRDYLRSGTILDAVNFPSFDRETSLLLR
                     PYMDLAVRLGGFVSQIVDGGHRRLVVRTYGTIGELSLRPLVLAAVKGLLGKAVEGGVS
                     YINALLLAKERGITVEEARSNEASAYAGLLRVRLETDTGHAEVGGTLLTADRPRLVEI
                     DGLPIESTLDGHMLFIRNRDVPGVVGKIGTILGRDGVNIAGIHLGRTDGTNGAVSIVD
                     VDSRPSESAITEIGSFDEITLVRAVAV"
     gene            complement(2169..2783)
                     /locus_tag="VD788_05565"
     CDS             complement(2169..2783)
                     /locus_tag="VD788_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HXV75769.1"
                     /translation="MRRSLERALRVALGTTRNNPLRPSADRLHALAAAATGAVGGMFG
                     LPGLIVELPATTVIMLRSIAEVARSDGADLDDPINRVHCLEVFALGRTSREAAETGYF
                     AARGAIAAAVGEAAHHVATRGISSRGAPALVRLVDTIATRFGVVVQEKLVLELLPLIG
                     AASGAAINTVFIAHFQHTARGHFIVRRLEDTYGPERIRESYRTL"
     gene            complement(2836..>3533)
                     /locus_tag="VD788_05570"
     CDS             complement(2836..>3533)
                     /locus_tag="VD788_05570"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXV75770.1"
                     /translation="WLDDRTVPLFDNVAKILLFGAAVYVLLLTWNLDVKPWLASAGIV
                     GVALGFAAKDTLANLFGGLFIIIDAPYKIGDFINLDTGERGQVTKIGLRSTRLLTRDD
                     VEITLPNAQIANAKIVNESGGPSQGFRVEIRVGVAYGSDVDQVRRVLLEAAGAVEIAA
                     ESPTPRVRFLEMGDSALIFRVLCWVDEPVSRGICIDALNTEVYKRLRQAGIEIPFPQR
                     ELHLRRPLAVEPA"
BASE COUNT          566 a         1191 c         1239 g          537 t
ORIGIN      
        1 ctcgatgcga cgaccgggga tctcgagccg gcggtgcgac aggcgatcga tcgcctgcgt
       61 ccggccctcg aatcgaccgg ggtgacggtc gagctcgagg tgcggccgcc ggttccggcg
      121 gcggtcttcg accgcgaggc cctgcaccag atcctgcaga acctgctcga caactcgtcg
      181 aagttcaacc gcgatgcgac cgaccggacc gtgcgcgtcc acctcgccgc cccggccgga
      241 cggccgaccc tcgaagtgat cgaccgcggg agcggcgtgg cgcgcgaggt cagacgcaag
      301 ctgttccgcc cgttctccca ccacccggac cccggcgcgc ccgccggtct cggtctgggc
      361 ctggcgatcg tccgagcgct ggccgccgcc caaggggccg aggtctcctt ccgggatcga
      421 ccggggggcg gggcggtctt ctcggtccgt ttcgatcgtg ggagctgaag cggatcggcc
      481 cggcccgggc tgatacaatc acgccgtctc gcgcgccgtc gtgccgccga ccggagaaag
      541 cgccatgtcc tcgcaaaacc cgaacccgcc ggctcgtgtg ctgatcgccg acaacctctc
      601 cgagaacggc gtcgagctgc tgcgcggctc acccgacatc gaagtgacgg tcaagatcgg
      661 catgacgccc gacgagctcg gcgtgacgat cggcgactac gatgcgctcg tcgtccgctc
      721 ggcgacgaag gtaaccgcgg aggtgctcga acggccgggg cgcctgcgcg cgatcggtcg
      781 cgccgggacg ggcgtggaca atatcgacct cgaggccgcg acgcgcgccg gcgtcgtggt
      841 gatgaacacg cccggcggca actccgtcgc cgcggccgag ttgacgctcg cgcacctgat
      901 cgcgctcgcg cggcacctgg tccaggccaa ccacgagctg cgacggggac ggtgggagcg
      961 caagaagtac gtcggtagcg agatcgaagg caagacgatc ggtatcgtcg gcctcgggcg
     1021 gatcgggcgc gaggtggcgc ggcgggccaa agggctgcgc atggaggtcg tgggctacga
     1081 cccgtacgtc tcgaccgacg cgtcggccgg cttcggcgtc cgtggcgtca cgctcgacga
     1141 gttgctggcg caggccgact tcgtcacgct tcacctgccg ctcagcgaca acacccgtca
     1201 cctgatcgat gcgagcaagc tcgccaagat gaaacgcggg gcccggttga tcaactgtgc
     1261 tcgcggcggg ctggtcgacg aggacgcgtt gttcgacgcg ctcgagtcgg gacagctggc
     1321 cggtgcggcg ctcgacgtgt tcgaggccga gccgccggcc gatcgtcggc tggtcgatca
     1381 tccgttggtc gtcgcgacac cccatctcgg cgcgtcgacg cgggaggccc aagagcgcgt
     1441 cggcagcgag atcgccgaga agatccgtga ctatctgcgt agcgggacga tcctcgacgc
     1501 cgtcaacttc ccctcgttcg atcgggaaac gtcgctcctg ctgcggccct acatggacct
     1561 tgccgtgcgc ctgggcgggt tcgtttcgca gatcgtcgac ggcgggcacc gccggctggt
     1621 cgtccgaacc tacgggacga tcggcgagct gtcgctgcgt ccgctggtgc tggcggcggt
     1681 caagggactg ctgggtaagg cggtcgaggg cggcgtctcg tacatcaacg cgctgctgtt
     1741 ggcgaaggaa cggggcatca ccgtcgagga agcgcgaagc aacgaggcgt cggcctacgc
     1801 cggcctgctg cgcgtccgtc tcgagaccga taccggacac gccgaggtcg gcggcacgct
     1861 gctcaccgcc gacaggccgc gactcgtcga gatcgacggg ctgccgatcg aatcgacgct
     1921 cgacggccac atgctgttca tcaggaatcg cgacgtgccg ggagtcgtcg gcaagatcgg
     1981 tacgatcctc ggacgtgacg gcgtcaacat cgccgggatc catctgggcc gtaccgacgg
     2041 gacgaacggc gcggtctcga tcgtcgacgt cgactcgcgg ccgtccgaga gcgcgatcac
     2101 cgagatcggc tcgttcgacg agatcacgct cgtgcgtgcg gtcgccgtct gagagcgaat
     2161 accgcggttc acaaggtgcg gtagctctcg cggatcctct ccgggccgta agtgtcctcc
     2221 agtcgccgga caatgaaatg cccgcgcgcg gtatgctgga agtgcgcgat gaacacggta
     2281 ttgatcgcgg cgccgctggc cgccccgatc aacgggagca gctcgaggac cagcttctct
     2341 tggacgacga ccccgaatcg cgtcgcgatc gtgtcgacca atcgaaccag tgccggcgca
     2401 ccgcgcgagc tgatgcctcg tgtcgcgacg tggtgcgccg cttcgccgac cgcggcggcg
     2461 atcgcgccgc gcgcggcgaa gtagccggtc tcggctgcct cacggctcgt tcggcccagg
     2521 gcgaatacct ccagacaatg gacccggttg atcgggtcgt cgaggtccgc cccgtcgctg
     2581 cgggcgacct cggcgatcga gcggagcatg atcacggtcg tcgccggcag ctcgacgatc
     2641 agaccgggga ggccgaacat cccaccgacc gcgccggtgg cggcggcggc cagtgcgtgc
     2701 aggcggtcgg ccgacggtcg tagcggattg ttgcgcgtgg tgccgagcgc cacgcgcagc
     2761 gctcgctcga gcgaccggcg cacgaccgca gctcgtcgag atcttcgagc atcagctcgg
     2821 gcatcgagcg gtcgctcagg cgggctcgac ggccagcggc cgtctcaagt gcagctcgcg
     2881 ctgcggaaac ggaatttcga tgccggcctg ccgcagccgc ttgtacactt cggtgttcag
     2941 cgcgtcgatg cagatcccgc gagagaccgg ctcgtcgacc cagcacagca cgcggaagat
     3001 cagcgcggaa tcgcccatct cgagaaaacg gacgcgcggc gtcggactct cggcggctat
     3061 ctcgacggcg ccggccgctt cgagcagcac gcggcggact tgatcgacgt ccgagccgta
     3121 ggcgacccct acccggattt cgacgcggaa gccctgagac ggaccacccg actcgttgac
     3181 gatcttcgcg ttggcgatct gcgcgttggg cagcgtgatc tcgacgtcgt cgcgcgtcaa
     3241 cagccgagtg ctgcgcaggc cgatcttcgt cacctgcccg cgctcgccgg tgtcgaggtt
     3301 gatgaagtcg ccgatcttgt acggcgcgtc gatgatgatg aacaggccgc cgaacagatt
     3361 ggccaacgtg tctttcgcgg cgaaaccgag tgcgacaccg acgatcccgg ccgaggcgag
     3421 ccacggcttg acgtcgaggt tccaggtaag caacagcacg tagacggcgg cgccgaacag
     3481 caagatcttg gccacgttgt cgaacagcgg caccgtccgg tcgtcgagcc acg
//
LOCUS       DASPGN010000196         6435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1320388, whole genome shotgun sequence.
ACCESSION   DASPGN010000196 DASPGN010000000
VERSION     DASPGN010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320388"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VD788_05575"
     CDS             complement(<1..247)
                     /locus_tag="VD788_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75771.1"
                     /translation="MSFVRLAPVICLSSLSVVAPATAEPPAERRFAELMALTDAGDVE
                     RIAAYVRENFVEAMQRAVPGDSGIVAFLADHASRFGGF"
     gene            complement(316..1290)
                     /locus_tag="VD788_05580"
     CDS             complement(316..1290)
                     /locus_tag="VD788_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HXV75772.1"
                     /translation="MIHAYVAGPAAVERREVAAGEALPDGAVWLDLLEPTEAERALVE
                     RAVEVEFPTRAEMVEIEPSSRLYTDRGAIFMTATIISHADRPNPGSDVITFILARGML
                     VTLRYVHPKPVATFAVRLERQPALCATSEAAFLGLIDSFVDRIADILERVGVDLDNLS
                     QQIFRDPAADGGDKPDLHAMLRTLGRNDDLTSTAAESLLSLSRLLRFFQQATDGTARK
                     EQKARIKDMQNDLLWLRQHVDSEASKVGFLLDATLGLINIEQNAIIKIFSVAAVVFLP
                     PTLVASIYGMNFRWMPELESPFGYPGALILMVLSAVLPYAFFKRKGWL"
     gene            1434..2324
                     /locus_tag="VD788_05585"
     CDS             1434..2324
                     /locus_tag="VD788_05585"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75773.1"
                     /translation="MSRIVRNTWIARAVLLASLGATAPARGAEIMIQASEDSWIDGLA
                     STATHGAELALGICPVANYWMYFKFDLSPVGGAVDAAELRLTRIDGDRPEEISLYLIT
                     DDGWSETTLNGPGRPAPTDPPNASALAQGEVDAGYDRWTSAALAATVEVERTGDGTLS
                     LMARENPAQMFDIRHYRSTESGFPAPQLPQLVVSCSTGEVASFVATSTDPATFTWSSG
                     TAWDLIGGTLADLRADLGVSSAGCLADALPEPTFTDTRPDPGAGELFYYVVRGDGPCG
                     TGSYGFASGGAERVPAADCP"
     gene            2321..3157
                     /locus_tag="VD788_05590"
     CDS             2321..3157
                     /locus_tag="VD788_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75774.1"
                     /translation="MTAARRSLAGAVLGVLLASAVHAGAWTPPRATAYTRFSVERFTA
                     DEGFDAEGEVVPFEFDGRYTDDTLKLYGEFALRDELALIASIPLQSIEYENRFESGAT
                     DGLGDFELGLRLRFVDRPVVVSFQALVRVPGHYEGEPRLPLGYDQYDAEGRLLLGQSL
                     AAGRIYYGLEAAYRWRDREPADEWRYLVELGGLLTRRLYARTKLEAIESAENAEDVRD
                     VFGNPVLRFESELRRIETTLGYRFARNLGVEVAWNPILDGRSTAKGTTWSLALVWQAR
                     LP"
     gene            complement(3158..4117)
                     /locus_tag="VD788_05595"
     CDS             complement(3158..4117)
                     /locus_tag="VD788_05595"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXV75775.1"
                     /translation="MKLALLAACCTLSVFAMANVATSLLLAWHLRARGQRWLLRSAAG
                     VLFARCLPAVSSAALALLIALPSFLAHEPRDRVEPVSLPLAVVAAFGLAILGGSVLRG
                     ASAVARTRTAVREWMREATPAVVPGWAGRAWIVAAEAPGVCVVGLREARLLVSRSTIA
                     ACTAPELAGIVAHERAHARAGDNRKKLVLRCLPDVVSWTPLSRAVERIWDARAEEEAD
                     VAALRADRTAGVEIAAALVKLARRGTFAALPVASTLDAGGPLGRRVRRLLAGTEERRS
                     RGSHWALLGAAIVVPAVVCALFPPASASVHQAIEAMVGQLLRF"
     gene            complement(4114..4638)
                     /locus_tag="VD788_05600"
     CDS             complement(4114..4638)
                     /locus_tag="VD788_05600"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXV75776.1"
                     /translation="MTADDSPRRSRRGRWIERFLTRRVADRPAAGPAGLLEVSFGPLE
                     LRVLDALWRRADEAGVRAIEAEFPGTAYTTLMTTLDRLFKKDLLVRRKVGRAYRYAPR
                     YGRAELEALLARDAVEELLDGARPGRGPLPVLSGFVEAVGRRDAQMLDELERLVREHQ
                     TATHRRRKRNGGEE"
     gene            4713..>6435
                     /locus_tag="VD788_05605"
     CDS             4713..>6435
                     /locus_tag="VD788_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXV75777.1"
                     /translation="MSRSPQARTLLALFAVVPWITPMQAAEPPAVETDAQRLVFLVQY
                     VGADYGLAVADGRIIDAFEYDEMVRFSGLLVRSIDGLVARGAPETVGDGLRRLERAIR
                     ALRPWDEVRSLADDLAAILLERLDLVPWPTTAPDPTRGGELYRTLCASCHGETGRGDG
                     PLAAGQDPPPTSFRDARMDLLSPHQIFGATRFGIDGTAMASYADLLSSSDTWDLAFHL
                     LTLREDFAPAAAAPDVELSLAELVVSSNAALLARVGQDRRAAVDYYRASPPAPAAPVL
                     AGPRRDADSQAAAIDATAPLDLALQLQDAFAGVAERMLPSVANVTTYVRTGDRAAEAA
                     PERDEAWAVAGSAALAYPGFRRLRSGSGFFVSRDGYLLTGHHVVVREDGEPAEVIDVE
                     LPGGRRVLSRLVGAEPTLDLALLKLEVVSEHRPPEVVPVAIGDAGSLRVGHWVIAVGD
                     PSGPERTYAVGTLAARADRQCYQEQRSATLLQASLVLHRESYGGPLVDIRGRVVGMLV
                     PPPEAAATNATEYALPIELAINISDALKVVESRRSPWLGVSVLELASARRSRAAEATP
                     PDLPPTGV"
BASE COUNT          916 a         2263 c         2334 g          922 t
ORIGIN      
        1 cgaacccgcc gaagcgagac gcgtgatccg cgaggaacgc gacgatgccg ctgtcgccgg
       61 gtacggcccg ttgcatcgct tcgacgaagt tctcccgcac gtaggcggcg atgcgctcga
      121 cgtcgccggc gtcggtcagc gccatcagct cggcgaagcg ccgctcggcc ggcggctccg
      181 ccgtcgctgg cgccaccacg gaaagactcg acagacagat caccggggcc agccgaacga
      241 agctcatcga cgcgtgctcc tcgggcgtga tacgcgcggg ccacgcccgc gggcgaggtc
      301 cgcgtccgcc cgccgctaga gccagccctt ccgtttgaaa aaggcgtagg gcagcaccgc
      361 cgacaggacc atcaggatca gcgcccccgg gtagccgaac ggagattcga gctcgggcat
      421 ccagcggaag ttcatcccgt agatgctggc gaccaacgtc ggcgggagaa agaccacggc
      481 cgcgacggag aagatcttga tgatcgcgtt ctgctcgatg ttgatcagac ccaaggtggc
      541 gtcgagcagg aagcccactt tgctcgcctc ggagtcgacg tgctgccgca gccacagcag
      601 gtcgttctgc atgtccttga tccgtgcctt ctgctccttg cgagcggtcc cgtcggtcgc
      661 ttgctggaag aagcgcagca gtcgactcag ggagagcagg ctctcggccg ccgtcgaggt
      721 gagatcgtcg ttgcggccca gcgtgcgcag catcgcgtgc aggtccggct tgtcgccgcc
      781 gtccgcggcg gggtcgcgga agatctgctg cgagaggttg tcgaggtcga cgccgacccg
      841 ctcgaggatg tcggcgatcc gatcgacgaa gctgtcgatc agaccgagaa aggccgcctc
      901 ggaggtggcg cagagtgccg gctgtcgctc gagccgcacg gcgaaggtcg cgaccggctt
      961 ggggtggacg taacgcagcg tgacgagcat gccgcgggcg aggatgaagg tgatcacgtc
     1021 gcttcccggg ttgggccggt cggcgtggga gatgatcgtc gcggtcatga agatcgcgcc
     1081 gcggtcggtg tagaggcggc tcgacggctc gatctcgacc atctcggccc gggtcgggaa
     1141 ctcgacctcg accgcgcgct cgacgagggc gcgctccgct tcggtcggct cgagcagatc
     1201 gagccacacc gcaccgtcgg gaagcgcctc ccctgccgcg acctcgcgtc gttcgactgc
     1261 agccggaccg gcgacgtacg catggatcat cgcgacctcc gtgatgcggc gccgtcacta
     1321 tagcggaccc cccgccgcct cgtgacccgc gtcggtcgcg aaccgcgcgg ggggaatatc
     1381 gtggcgcacc gtccggtttc gcagtaccac gttcgcaggc gcggggaggt gcgatgtcca
     1441 ggatcgtccg caacacgtgg atcgctcgcg cggtcttgct cgcttcgctc ggcgccaccg
     1501 ccccggcgcg cggggcggag atcatgatcc aggccagcga agacagctgg atcgacgggc
     1561 tcgccagcac ggcgacgcac ggcgccgaac tggcgctcgg gatctgcccg gtcgcgaact
     1621 actggatgta cttcaagttc gacctgtcgc cggtcggtgg tgcggtcgat gcggccgagc
     1681 tgcgactgac gcggatcgac ggcgaccggc ccgaggagat ctcgctctat ttgatcaccg
     1741 acgacggctg gagcgagacg acgctgaacg gtcccggccg cccggcgccg accgacccgc
     1801 cgaacgcgtc ggcgctggcc cagggggagg ttgacgcggg ctacgatcga tggacctctg
     1861 cggcgctcgc cgcgacggtc gaggtggaac ggaccggcga cggcacgttg tcgctgatgg
     1921 cgcgcgagaa cccggcgcag atgttcgaca tccgccacta ccgcagcacc gagtccggtt
     1981 ttcccgcccc gcagctcccc cagctggtcg tcagctgctc gaccggcgag gtggcgtcgt
     2041 tcgtcgccac gtcgaccgac cccgcgacct tcacctggtc atcggggacg gcgtgggatc
     2101 tgatcggcgg cacgctcgcc gacctgcgcg ccgacctcgg ggtgtcgtcg gccggctgcc
     2161 tcgcagatgc cctgcccgag ccgacgttca ccgatacgcg gcccgacccg ggggccggag
     2221 agctgttcta ctacgtcgtg cgcggcgatg gtccgtgcgg caccggcagc tacggcttcg
     2281 ccagcggcgg tgcggagagg gtcccggcgg cggattgccc gtgacggctg cccggcgctc
     2341 gctcgcgggt gcggtgctcg gcgtgctgct ggcgtcggcg gtgcacgccg gtgcctggac
     2401 cccgccacgc gctacggcct acacgcgctt ctcggtcgaa cggttcaccg ccgacgaggg
     2461 attcgacgcc gagggcgagg tcgtaccctt cgagttcgac ggccggtaca ccgacgatac
     2521 gctgaagctc tacggcgagt tcgcgttgcg cgacgagctg gccttgatcg cttcgatccc
     2581 gctgcagtcg atcgagtacg agaatcggtt cgagtcgggc gcgacggacg ggctcggcga
     2641 cttcgagctg ggcctgaggc tgcgcttcgt cgatcgaccc gtcgtcgtgt cgtttcaggc
     2701 cctggtgcgg gtaccgggtc actatgaagg cgagccgcgg ttgccgttgg gctacgacca
     2761 gtacgacgcc gaagggcggt tgttgctcgg ccagtcgttg gccgccgggc gcatctacta
     2821 cgggttggag gccgcctacc gctggcgcga tcgcgagccg gcggacgaat ggcgctacct
     2881 cgtcgagctg ggcggccttc tcacgcgtcg gctctacgcc aggaccaagc tcgaggcgat
     2941 cgagagcgcc gaaaacgccg aggacgtccg cgacgtcttc ggcaacccgg tgctccgctt
     3001 cgaaagcgag ctgcgtcgca tcgaaacgac gttgggctac cgcttcgcgc ggaatctcgg
     3061 ggtggaggtc gcttggaacc cgatcctcga cgggcgtagc acggccaagg ggacgacctg
     3121 gtcgctcgcc ctggtctggc aagcgcggct gccgtaatca gaatcggagc agctgcccca
     3181 ccatcgcctc gatcgcctgg tgcacggagg ccgacgcggg cgggaaaaga gcgcagacga
     3241 ccgccggcac cacgatcgcc gcgccgagca gcgcccagtg cgagcctcgc gaacgccgct
     3301 cctcggtccc ggcgagcagg cgtcgaacgc gccggccgag cggcccgccc gcgtcgagcg
     3361 tgctcgccac gggcagcgcg gcgaacgtcc cgcggcgggc caacttgacc agcgcggcgg
     3421 cgatctcgac gccggccgtc cgatcggcgc gcaacgccgc gacgtcggcc tcttcttcgg
     3481 cgcgcgcatc ccagatgcgc tcgaccgcgc gcgacagcgg cgtccacgag acgacgtcgg
     3541 gcagacaacg cagcaccagc ttcttccggt tgtcgcccgc gcgggcgtgc gcgcgctcgt
     3601 gggcgacgat cccggcgagc tccggcgccg tgcacgcggc gatcgtgctg cgcgagacca
     3661 agagtcgcgc ttcgcgcagg ccgacgacgc agacgcccgg cgcttcggcg gcgacgatcc
     3721 aggcccgacc ggcccagccc gggacgacgg cgggtgtcgc ctcgcgcatc cattcgcgga
     3781 cggcggtgcg cgtccgggcg acggcgctcg cgccgcgcag gacggatccg ccgaggatcg
     3841 cgagcccgaa cgccgccacg accgcgagcg ggaggctcac cggctccacc cgatcccgtg
     3901 gctcgtgggc caggaacgac ggcaacgcga tcagcagggc cagcgcggcc gacgagaccg
     3961 cgggcagaca gcgggcaaac agcactcccg ccgccgagcg aagcagccag cgttggccgc
     4021 gcgcacgcag gtgccaggcc agcaggaggg aagtcgccac gttcgccatc gcgaagacgg
     4081 acagcgtgca gcaggccgcg aggagcgcga gcttcattcc tctcccccgt tccgcttccg
     4141 cctgcggtgc gtcgccgtct ggtgctcgcg caccaggcgc tcgagctcgt cgagcatctg
     4201 ggcatcccga cggccgaccg cttcgacgaa gccgctcagc acgggcagcg gtccgcggcc
     4261 cggccgggcg ccgtcgagca gctcctcgac ggcgtcgcgg gcaagcaacg cttcgagctc
     4321 ggcgcggccg tagcgcggtg cgtagcggta ggcgcgaccg accttgcgtc gcacgagcag
     4381 atccttcttg aacagtcgat cgagcgtcgt catcagggtc gtgtaggcgg tcccggggaa
     4441 ctcggcctcg atcgcgcgca cgccggcctc gtccgcgcgc cgccacagcg cgtcgaggac
     4501 gcgcagctcg agcgggccga acgatacctc gagcaggccg gccgggccgg ccgcggggcg
     4561 gtcggcgacg cggcgcgtca ggaaccgctc gatccagcgg ccgcgacgcg atcggcgggg
     4621 cgagtcgtct gcggtcatgc ggccaccagg ataaccggaa tcggatcctc cggcttgtcg
     4681 ccgagcgagg aacgggaata ggctaggggg tcatgtcgcg ctcgccgcaa gcccgaacgc
     4741 tgctcgctct gttcgcggtc gtcccgtgga tcacgccgat gcaggccgcc gagccgccgg
     4801 cggtcgagac ggacgcccag cggctggtct ttctcgttca gtacgtcggg gcggactatg
     4861 gcttggcggt ggcggatggt cggatcatcg acgcgttcga gtatgacgag atggtccgct
     4921 tctccggctt gctcgtccgc tcgatcgacg gactcgtcgc acgcggagcg cccgagacgg
     4981 tcggtgacgg cctgcgccgc ctcgagcggg cgatccgcgc gcttcggccg tgggacgagg
     5041 tgcgctcgct ggccgacgac ctggccgcca ttctgctcga gcggctcgac ctcgtgccgt
     5101 ggcccaccac cgcgcccgac ccgacgcgtg gcggcgagct gtaccggacg ctctgcgcgt
     5161 cgtgccacgg tgagaccggt cgaggcgacg gcccgctggc ggccgggcag gatccgcctc
     5221 ccacctcgtt ccgcgacgcg aggatggatc tcctctcgcc gcatcagatc ttcggcgcga
     5281 cgcggttcgg catcgacggc accgcgatgg cctcctacgc cgatctgctc tcgtcgagcg
     5341 acacgtggga cctggccttt cacctgctga ccctgcgcga ggacttcgcg ccggcagcgg
     5401 cagcccccga cgtcgagctg tcgctcgcgg agctcgtggt gagctcgaat gccgcgctgc
     5461 tcgcgcgagt cggccaggac cggcgggccg cggtcgacta ctatcgggcc tccccgcccg
     5521 ccccggctgc gccggtgctc gccggccccc ggcgggacgc ggattcacag gcggcggcga
     5581 tcgacgcgac cgcgccgctc gatctcgcgc tgcagctgca ggacgccttc gccggcgtgg
     5641 cggagcggat gctgccgagc gtggcgaacg tgacgaccta tgtgcgcacc ggggatcgag
     5701 cggcagaagc cgcgccggag cgcgacgagg cgtgggccgt ggcgggttcg gccgcgctcg
     5761 cctatcccgg cttccgccgt ctccgctcgg gtagcggctt cttcgtcagc cgcgacggct
     5821 acctgctgac cgggcatcac gtcgtcgtgc gcgaggacgg cgagccggcg gaggtcatcg
     5881 acgtcgagct gccgggggga cgccgggtgc tgagtcggct cgtcggcgcc gagccgacgc
     5941 tcgacctggc gctgctgaag ctcgaggtcg tctccgagca ccggccgccc gaggtggtgc
     6001 cggtcgcgat cggcgacgcc ggctcgctgc gcgtcgggca ctgggtgatc gcggtcggcg
     6061 acccgtcggg gcccgagcgg acctacgccg tcggcaccct cgccgcgcgg gccgatcgcc
     6121 agtgttacca ggagcaacgc agcgcgacgc tgctacaggc gtcgctcgtc ctgcaccgag
     6181 aaagctacgg cggcccgctc gtcgacatcc gcggtcgggt cgtcggcatg ctcgtgccac
     6241 cgcccgaggc ggccgcgacg aacgcgacgg agtacgcgct gccgatcgag ttggcgataa
     6301 acatctccga cgcgctcaag gtcgtcgaaa gccggcgctc gccgtggctc ggggtgtccg
     6361 tgctcgagct ggcctcggcg cgccggagcc gggcggcgga ggcgacaccg cccgatctcc
     6421 cgccgaccgg tgttt
//
LOCUS       DASPGN010000197         5601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1438117, whole genome shotgun sequence.
ACCESSION   DASPGN010000197 DASPGN010000000
VERSION     DASPGN010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438117"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="VD788_05610"
     CDS             <1..1113
                     /locus_tag="VD788_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV75778.1"
                     /translation="CDDFGHGTHTMGTIVGDDGATNQIGVAPSARWIGCRNMHRSVGT
                     PQSYTECFQWFVAPTDLDGDDPDPALAPDVINNSWSCPSSEGCSQSTLRTIVENTRAA
                     GIVVVASAGNAGADCGSVATAPAHYAAALTVGATGTTDLIAPFSSRGPVLVDGSHRLK
                     PDLVAPGVTVRSSVPGDDYDVNSGTSMAAPHVVGVTALLLSARPDLAGRVDEIEAILR
                     ASTVPLTTTEGCGGDSGVDVPNNTYGAGRIDAVAAITGDADGDLVVNLDDCAPANGSW
                     WSVPSPARNLTLEGGTLALISWQPPLEPGAATVQYEVLRSERADDFSNAVCVAGPQKT
                     TAASDAAVPSTSFYYKVRVHNACGAAGPDAPSCAAQ"
     gene            complement(1142..1693)
                     /locus_tag="VD788_05615"
     CDS             complement(1142..1693)
                     /locus_tag="VD788_05615"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75779.1"
                     /translation="MSSDLVERLPIVGVMGSGRAPHLDRAEPLGRWLAGAGVHLLTGG
                     GGGVMTAVSRAFHRVVPRAGRVIAILPGSVDGTGYTTRAGYPNPWVEIPIATHLPLSG
                     ARGTETMSRNHINVLTADLIVALPGGAGTSSEVALAVAYGRPVVVFAGDRRAIPGLAG
                     DVPLEPRFEGVEAFVRRVLDRES"
     gene            complement(1690..2520)
                     /locus_tag="VD788_05620"
     CDS             complement(1690..2520)
                     /locus_tag="VD788_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXV75780.1"
                     /translation="MRLALVQQAAGPDKSDNVRRALTTLERAAAAGAELVCYAELSFE
                     RFYPQLPARAGFEQLAETIPGPLTDRFAAAAAEHRVVVVANLFERDGDRCYDTSPVID
                     ADGSLLGRTRMVHITEYDCFHERGYYTPGDLGAPVFDTAVGKLGVAICYDRHYPEYMR
                     ALALGGAELVVVPQAGSVGEWPDGLYEAEMRVAAFHHGYHVALCNRVGREERLEFAGE
                     SFVCDPAGRIVARAAAGTDDLLLVDIEPGAAADSHARRLFLRDRRPELYGAWLGPRPR
                     "
     gene            complement(2483..4402)
                     /locus_tag="VD788_05625"
     CDS             complement(2483..4402)
                     /locus_tag="VD788_05625"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXV75781.1"
                     /translation="MTPDHASHAAFSVAVALATGMVAQSLARHLRIPGIVLLLAAGVL
                     LGPDALDMIDPKSLGSALQVLVGFAVAVILFEGGLNLSLSAIRRQVGVIPRLITVGAL
                     VTAVGGTLLARLVMGWSWSLSALFGALVVVTGPTVITPLLRRIKVTHRVETILEAEGV
                     LIDAIGAVLAVVTLEVVLSFSAASLAEGAMRLVELLGFGLIAGLFGGLAIAFLLRFNR
                     VVPPGFENVLTLSLVLALYQACNAVMAESGIMAVTAAGVVVGNVRTRALRDLMEFKEQ
                     LTMMFIGLLFVLLAADVRIRDVLGLGWPGVWTVAGLMFLVRPLNIWLCTLGSDLTHRE
                     KVFLAWLAPRGVVAAAVASLFAQTLDNAGVPGGGDLRALVFLVIAITVNVEGSTGGLV
                     ASLLGLRRAARRGYVILGANALGRTLGRLLVDAGIEVVIIDNNPAQVRVAETAGFRVI
                     YGNAVEERTLLRAGIDERVGAIGLTPNDEVNLLFARAAHDDYRLRENYIALRHGPGPI
                     SSSVVEAHEAQVLFGKPRDLVTWMVRIRRDLCRIERFTLVTTGGSADAESPFAFPEQL
                     ILPLALYRGSKIYPIGSARKLKKGDVIAFALFAEELAQARRWLSDNHWQEEAQADRPA
                     EVERETGTGSTSRGS"
     gene            complement(4399..4785)
                     /locus_tag="VD788_05630"
     CDS             complement(4399..4785)
                     /locus_tag="VD788_05630"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXV75782.1"
                     /translation="MTARSRFGGNAPWESIVGYSRAIRSGDAIHVSGTTALDEHGRLV
                     GERDADALTRRILGSIADVLDRAGAGVEHVVRTRIYVTDVSRWQEIGRAHGEFFRDVR
                     PTTAMVEVSALIDPGMLVEIEVEARP"
     gene            complement(4809..5417)
                     /locus_tag="VD788_05635"
     CDS             complement(4809..5417)
                     /locus_tag="VD788_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXV75783.1"
                     /translation="MIEALGGLIDGGRIKVYCTESNVAEAWTRRDGDPRWRIQRHLAF
                     EHYIVAELAPRIREDCHTPDIPIATTGTSLGAFYAANFALKYPSLFNYALCMSGRYDT
                     SEFTGGLASTDTYFNNPMAYVPNLHGDVLQRVRGAIHVTLVCGQGRWEDGNVDEARTF
                     ARMLAAKEISHQLDIWGHDVSHEWQWWRRQALHHLSGRFGSG"
BASE COUNT          951 a         1974 c         1876 g          800 t
ORIGIN      
        1 tgcgacgact tcggacacgg gacgcatacg atggggacga tcgtcgggga cgacggcgcg
       61 acgaatcaga tcggcgtcgc gccctccgcg cgctggatcg gctgccgcaa catgcaccgc
      121 agcgtcggca cgccgcaaag ctacaccgag tgcttccagt ggttcgtcgc gccgaccgat
      181 ctcgacggcg acgatcccga tccggcgctg gcgccggacg tgatcaacaa ctcctggagc
      241 tgcccgtcgt cggagggatg ctcgcagtcg acgctgcgga cgatcgtcga gaacacgcgc
      301 gccgcgggga tcgtcgtcgt ggccagtgcc gggaatgccg gagcggattg cggcagcgtg
      361 gcgacggctc cggcgcatta cgcggcggcc ctgaccgtcg gcgcgacggg cacgaccgac
      421 ctgatcgcgc cgttcagcag tcgcggtccg gtcctcgtcg acggcagcca ccggctcaag
      481 ccggatctcg tcgcgcccgg tgtcaccgtt cgttcgagcg ttcccggcga cgattacgac
      541 gtcaacagcg ggacgagcat ggcggcgccc cacgtcgtcg gggtcaccgc actgttgctc
      601 agcgcgcgcc cggatctcgc gggtcgggtc gacgagatcg aggcgatcct ccgcgcgtcc
      661 accgtcccgt tgacgacgac cgagggttgc ggcggcgaca gcggggtcga tgttccgaac
      721 aacacgtacg gcgccgggcg gatcgacgcc gtcgcggcga tcacgggcga tgccgacggc
      781 gacctggtgg tcaacctgga cgactgtgcg ccggcgaacg gcagctggtg gtcggtgccg
      841 tcgccggcgc gcaacctgac gctcgaaggc ggtactctgg cgttgatcag ctggcagccg
      901 ccgctcgagc ccggcgccgc caccgtccag tacgaggtgc tgcggagcga acgggcggac
      961 gacttctcga acgcggtctg cgtcgccggg ccgcagaaaa cgacggcggc gagcgacgcc
     1021 gccgtcccgt cgacgtcctt ctactacaag gtccgcgtcc acaacgcctg tggcgccgcc
     1081 gggcccgatg ccccgagttg tgccgcgcaa tgaccgcgac ccggtcctgc cccggcccgc
     1141 gtcagctctc gcggtcgagc acccggcgga cgaacgcctc gacaccttcg aatcggggtt
     1201 cgagcgggac gtcgccggcc aggccgggaa tcgcccggcg atcgccggcg aagacgacga
     1261 ccggccgccc gtaggccacc gccagcgcaa cctcgctcga cgtccccgcg cccccgggta
     1321 gcgcgacgat cagatccgcg gtcaggacgt tgatgtggtt tctcgacatc gtctccgttc
     1381 cgcgcgcacc gctcaacggc agatgcgtcg cgatcgggat ctcgacccac gggttgggat
     1441 agcccgcccg cgtggtgtac ccggtgccgt cgaccgaacc gggcaagatc gcgatcaccc
     1501 gtccggcgcg tgggacgacg cgatggaagg cgcggctgac ggcggtcatc acgccgccgc
     1561 cgccgccggt cagcaggtgt actccggccc cggcgagcca gcgcccgagc ggttcggccc
     1621 gatcgagatg cggcgcgcga cccgagccca tcacgccgac gatcggcaaa cgctcgacga
     1681 ggtcggagct catctcggtc gcgggccgag ccacgcaccg tacagctcgg gccggcggtc
     1741 gcgcaggaac aaccggcgcg cgtgcgaatc ggcggcggca cccggctcga tgtcgaccag
     1801 cagcaggtcg tcggttccgg ccgcggcgcg cgcgacgatg cgccccgccg ggtcgcagac
     1861 gaacgactcg ccggcgaact ccagccgctc ctcgcggccg acgcgattgc acagcgcgac
     1921 gtggtagccg tggtggaacg ccgcgacgcg catctcggcc tcgtacaaac cgtccggcca
     1981 ctcgccgacc gaccccgcct gcggcaccac gaccagctcc gccccgccga gcgccagtgc
     2041 ccgcatgtac tccgggtagt gacggtcgta gcagatcgcg acgcccagct taccgaccgc
     2101 ggtgtcgaat accggagcgc ccaggtcacc cggcgtgtag tagccccgct cgtgaaagca
     2161 gtcgtattcg gtgatgtgca ccatccgcgt cctgccgagc aacgatccgt cggcgtcgat
     2221 gaccggcgag gtgtcgtagc atcgatcgcc gtcgcgctcg aacagattgg ccacgacgac
     2281 cacccgatgc tcggcggcgg ccgcggcgaa gcgatcggtg agcggaccgg gaatcgtctc
     2341 ggccagctgc tcgaagccgg ccctcgccgg cagttgcggg tagaaccgct cgaacgacaa
     2401 ctcggcgtag cagaccagct cggccccggc cgctgcggca cgttcgagcg tcgtcagggc
     2461 gcgacggacg ttgtcgctct tgtcaggacc cgcggcttgt tgaaccagtg ccagtctcac
     2521 gctccacctc cgccggccga tcggcctgag cttcctcttg ccagtggttg tcggacagcc
     2581 aacgtcgtgc ctgcgccagc tcctcggcga acaaggcgaa tgcgatgacg tcgcccttct
     2641 tcagcttgcg cgccgagccg atcgggtaga tcttcgagcc gcggtagagc gccagcggca
     2701 ggatcagctg ctcggggaag gcgaacggcg actcggcgtc ggccgaccca ccggtcgtga
     2761 ccagcgtgaa ccgctcgatg cggcacagat cccgccgtat ccgcaccatc cacgtcacca
     2821 gatcacgcgg cttgccgaac agcacttgcg cctcgtgcgc ctcgacgacc gacgagctga
     2881 tcggccccgg gccgtggcgc agcgcgatgt aattctctcg cagccggtag tcgtcgtgtg
     2941 cggcgcgcgc gaacagcaga ttgacctcgt cgttgggcgt gagaccgatc gcaccgactc
     3001 gttcgtcgat tcccgcgcgc aacaacgtgc gctcctcgac tgcgttcccg tagatcactc
     3061 ggaagcccgc ggtctccgcc acgcgcacct gcgccgggtt gttgtcgatg atcacgacct
     3121 cgatgccggc gtcgacgagc aagcgtccga gcgtgcgacc aagcgcgttc gcgccgagga
     3181 tcacgtaacc gcgtcgggcg gcccgccgca gcccgagcaa cgacgcgacc aggccgccgg
     3241 tcgacccctc gacgttgacc gtgatcgcga tgacgaggaa gacgagcgct cggaggtctc
     3301 cacctcccgg cactccggcg ttgtccagcg tctgggcgaa caacgaggcg accgccgccg
     3361 ccaccacgcc gcgcggagcg agccaggcga gaaagacctt ctcccggtga gtcagatcgg
     3421 aaccgagcgt gcacagccag atgttgagcg ggcggacgag gaacatcagt ccggcgaccg
     3481 tccacacccc gggccagccc agcccgagca cgtcgcgaat ccgcacgtcg gccgcgagca
     3541 aaacgaacaa caggccgatg aacatcatcg tcagctgttc cttgaattcc atcagatcgc
     3601 gtaaggcccg cgtgcgcacg ttgccgacca cgacaccggc cgcggtgacc gccatgatgc
     3661 cgctttcggc catgacggca ttgcacgcct gatacagcgc cagcacgagg gagagtgtca
     3721 gcacgttctc gaatccgggc gggacgaccc ggttgaatcg cagcaggaat gcgatggcga
     3781 gaccgccgaa cagaccggcg atcaggccga atccgagcag ctcgaccagc cgcatcgcac
     3841 cttcggccaa cgaggcggcg gagaaactga gcaccacctc gagcgtgacg acggccagta
     3901 cggccccgat cgcgtcgatc agcactccct cggcctcgag gatcgtctcg actcgatggg
     3961 tgaccttgat gcgtcgcaac agcggcgtga tgaccgtcgg tccggtaacg accaccagcg
     4021 cgccgaacag cgccgacaac gaccaactcc agcccatcac cagccgcgcg agcagcgttc
     4081 cgccgaccgc ggtcacgagc gcgccgacgg tgatcagccg ggggatgacg ccgacctgac
     4141 gccggatcgc ggacaggctc aggttgagac ctccctcgaa caggatcacg gcgacggcga
     4201 agccgaccag cacctgcagc gccgacccga gcgacttcgg atcgatcatg tcgagcgcgt
     4261 cgggcccgag cagtactcct gccgccagca gcagcacgat gccggggatc cgcagatgac
     4321 gcgccaggga ttgcgccacc atccccgtcg cgagcgccac cgcgaccgag aacgctgcgt
     4381 gcgaagcgtg atcgggtgtc acgggcgtgc ttcgacctcg atctcgacca gcatgcccgg
     4441 gtcgatcaac gcgctcactt cgaccatggc ggtcgtcggc cgtacgtcgc ggaagaactc
     4501 gccgtgagcc cggccgatct cctgccagcg ggagacgtcg gtgacgtaga tccgtgtgcg
     4561 gacgacgtgc tcgaccccgg cgcccgcgcg atcgagcacg tcggcgatgc tgccgaggat
     4621 ccgtcgcgta agggcatcgg catctcgctc gccgaccagt cgtccgtgct cgtcgagcgc
     4681 ggtcgtaccg gaaacgtgaa tcgcgtcccc cgagcggatc gcacgcgagt agccgacgat
     4741 cgactcccag ggcgcgttac cgccgaatcg actgcgcgcg gtcatcgcca ccgcatcatg
     4801 cccacgactc agccggatcc gaagcgcccg gacaggtggt gcagcgcctg gcggcgccac
     4861 cattgccact cgtgcgagac gtcgtgcccc cagatatcga gctgatggct gatctccttg
     4921 gccgcgagca ttcgggcgaa cgtgcgagcc tcgtcgacgt tgccatcctc ccagcggccc
     4981 tgaccgcaga ccagtgtcac gtggatcgcc ccgcgcaccc gttgcagcac atcgccgtgg
     5041 agattgggaa cgtaggccat cgggttgttg aagtaggtat ccgtgctggc cagcccaccg
     5101 gtgaactccg aggtatcgta gcggccgctc atgcacaacg cgtagttgaa cagcgacgga
     5161 tacttcagcg cgaagttggc ggcgtagaac gctcccaggc tggtgccggt cgtcgcgatc
     5221 ggaatatcgg gcgtatggca atcctcgcgg atgcggggcg cgagctcggc gacgatgtaa
     5281 tgctcgaagg caaggtgccg ctggatccgc cagcgcggat cgccgtcgcg tcgcgtccat
     5341 gcttcggcaa cgttggactc cgtgcagtag accttgatgc gaccgccgtc gatcaacccg
     5401 ccgagggctt cgatcatgcc gtgcgcatcc cactcgtggg ccatgcccga ggcggacgga
     5461 aaaacgagta tgggcgcgcc gaagtgcccg tagcgccaca ggtgaatcgg tcggcccagg
     5521 gacgcgctcg gcacgaggtg gtgttgactc cccatcgcac cagtatatgc aaggtggtgc
     5581 gcagcttggt ggtcgggatt g
//
LOCUS       DASPGN010000198         2555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1673185, whole genome shotgun sequence.
ACCESSION   DASPGN010000198 DASPGN010000000
VERSION     DASPGN010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673185"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /gene="ftsA"
                     /locus_tag="VD788_05640"
     CDS             complement(<1..663)
                     /gene="ftsA"
                     /locus_tag="VD788_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXV75784.1"
                     /translation="MGRNERYVVGLDIGTTKICCVVGEVTESGSVSVVGLGEAPSRGL
                     RKGVVVNLDATVEAVRTAVAEAELMAGVSVESATVGIAGNHIRSFNSRGVVSVAARDR
                     TISREDVRRVMDAARAVSIPADREIMHVLPQEFVIDDHGGIAAPIGLVGSRLEANVHV
                     VTGATTSIQNLVSCANRAGIEVRDTVLEQLAVAESVLSEDERQLGVVLIDIGGGTTDL
                     AIF"
     gene            complement(667..1518)
                     /locus_tag="VD788_05645"
     CDS             complement(667..1518)
                     /locus_tag="VD788_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HXV75785.1"
                     /translation="MSRSRVGSIRVGPDQRYWRRRANRRVRKARRVVSLLRWSLTVGV
                     NGIVLAALLVAADRAYDYLTTIDEFAVRRIELSGLRHASPELIRARLSEQIDGNMLDL
                     DLARIEQTVQADPWVRRAAVRRVLPDTLYVHVEERIPVAIAIVHDREWLVDSTGYVIG
                     PLDAGVDGRFPVLDGLDAGDERALERDLRRGVTVLERLRATSEVFARGVARVDLAHPD
                     RVEVTQDAAGPKILLDPDRIEQNLIYFMALRATIERRVGAADYIDLRWRDRISVMPAK
                     HSARRSG"
     gene            complement(1515..>2555)
                     /gene="murC"
                     /locus_tag="VD788_05650"
     CDS             complement(1515..>2555)
                     /gene="murC"
                     /locus_tag="VD788_05650"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HXV75786.1"
                     /translation="AEVLTHAGLDPTVVIGGRVGKLRSGAKLGRGELLVAEADESDGS
                     FLKMKPTIAVVTNIDREHLDHYDNLGRIRDAFAEFLSLVPFYGATVVCLDDPNVRDIL
                     PRVDRKTISYGLSSDADVAATDVRIEGFRSTYLATRHGKPLGRIELALPGRHSVYNSL
                     AAIAVGLELDLPFHDIARGLAGFHGVDRRLQRRGTVGGATVLDDYGHHPTEIRATLAA
                     VREGFGSRTIVVFQPHRYSRTRALLEEFGAAFYLADRVIVTDIYPAGEEAINGINGSA
                     VADALVRHGHPWVRYQPEFDRILPLLRDALRPGDIVLTLGAGDVWKIGEQVVREAQRK
                     RKAGAKGARSDR"
BASE COUNT          404 a          896 c          861 g          394 t
ORIGIN      
        1 gaagatggcg aggtcggtgg tgccaccacc gatgtcgatc aacacgacgc cgagctgccg
       61 ctcatcctcg ctcaacaccg actcggcgac ggccagttgc tcgagcaccg tgtcacgcac
      121 ctcgattccc gcacggttgg cgcagctgac caggttctgg atcgaggtcg ttgcgccggt
      181 gacgacgtgc acgtttgcct cgagtcgcga gccgaccaaa ccgatcggcg ccgcgatgcc
      241 accgtggtcg tcgatgacga actcctgcgg cagcacgtgc atgatctctc gatcggcggg
      301 aatactgacc gcgcgcgccg cgtccatcac ccgtcgcacg tcctcgcgcg agattgttct
      361 gtcgcgcgcc gcgaccgaaa caacgccgcg gctgttgaac gagcggatgt ggttgccggc
      421 gatgccgacg gtcgccgact cgacgctgac tccggccatc agctccgcct cggcaacggc
      481 ggtcctgacc gcctcgacgg tcgcgtcgag gttgacgacg acgcccttgc gcaacccgcg
      541 cgagggcgcc tcgcccaatc ccaccacgct gaccgacccg gattcggtga cctcgcccac
      601 gacgcaacag atcttggtcg tgccgatgtc gagtccgacg acgtatcgtt cgttcctacc
      661 catggatcaa ccgctcctcc gggctgaatg tttggccggc atgaccgaga tccggtcgcg
      721 ccagcgaagg tcgatgtagt cggcggcgcc cacgcggcgc tcgatcgtcg cgcgcagcgc
      781 catgaagtag atcaggtttt gctcgatgcg gtccgggtcg agcaggatct tcggacccgc
      841 tgcgtcctgc gtcacctcga cgcggtcggg gtgggcgaga tcgacccgcg ctacgccgcg
      901 cgcgaagacc tcgctcgtgg cccgcagccg ctcgagcacg gtcacgccgc ggcgcaagtc
      961 gcgttcgagc gcccgctcgt cgccggcgtc gagcccgtcg agcaccggga agcggccgtc
     1021 gacgcccgcg tcgagcggac cgatgacgta tcccgtcgag tcgacgagcc actcgcggtc
     1081 gtggacgatc gcgatcgcca ccgggatccg ctcctcgacg tgcacgtaga gtgtgtcggg
     1141 caacacgcga cgcacggcgg cgcgacgcac ccacgggtcg gcttgcacgg tctgctcgat
     1201 tcgcgcgaga tcgagatcga gcatgttgcc gtcgatctgc tcggacaacc gcgcccggat
     1261 cagctcgggc gaggcgtgcc tcagaccgga cagctcgatg cggcggaccg cgaactcgtc
     1321 gatcgtcgtc aggtagtcgt aggcccggtc ggccgcgacc agcagcgccg ccagcacgat
     1381 gccgttgacg ccgacagtca acgaccagcg cagcagactg accacgcgtc gagccttgcg
     1441 tacgcggcgg ttggctcggc gccgccagta acgctggtcc ggaccgacgc ggatcgagcc
     1501 gactcggctc ctgctcatcg atcgctcctg gcgcctttgg cgccggcttt ccgtttgcgc
     1561 tgcgcctcgc gcacgacctg ctcgccgatc ttccacacgt cgcccgcacc gagcgtcagc
     1621 acgatgtcgc ccgggcgcag cgcgtcgcgc agcagcggca ggatgcggtc gaactcgggc
     1681 tggtagcgga cccacgggtg gccgtggcgc acgagcgcat cggccaccgc cgatccgttg
     1741 atgccgttga tcgcctcctc cccggccgga tagatgtcgg taacgatcac ccgatcggcc
     1801 aggtagaacg ccgccccgaa ctcctcgagc agggcccgcg tacgggagta gcggtgcggc
     1861 tggaacacga cgatcgtgcg cgagccgaac ccctcgcgca cggcggccag ggtggcgcgg
     1921 atctcggtcg ggtggtggcc gtagtcatcg agcacggtgg cgccgccgac cgtgccgcgg
     1981 cgctgcagtc ggcgatcgac cccgtggaac ccggccagac cgcgcgcgat gtcgtgaaac
     2041 ggtaggtcga gctcaaggcc gaccgcgatc gcggccagcg agttgtagac cgagtggcgg
     2101 ccgggcagcg ccagctcgat gcgacccagc ggcttgccgt ggcgcgtggc gaggtaggtc
     2161 gagcgaaacc cctcgatccg cacgtcggtc gctgccacgt ccgcgtcgct cgacagcccg
     2221 tagctgatcg tcttgcgatc gacgcgcggc aggatatcgc ggacgttcgg gtcgtcgagg
     2281 cagacgaccg tcgctccgta gaacggaacc agcgagagaa actcggcgaa cgcatcgcgg
     2341 atccgaccga ggttgtcata gtggtcgaga tgctcgcggt cgatgttggt caccaccgcg
     2401 atcgtcggtt tcatcttgag gaacgaaccg tcggactcgt cagcctcggc caccaacagc
     2461 tcgcctcgcc cgagcttggc gccggaacgc agcttgccga cgcgtccgcc gatgacgacg
     2521 gtcgggtcga gaccggcgtg cgtcagcacc tcggc
//
LOCUS       DASPGN010000199         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_150815, whole genome shotgun sequence.
ACCESSION   DASPGN010000199 DASPGN010000000
VERSION     DASPGN010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150815"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1226)
                     /locus_tag="VD788_05655"
     CDS             complement(42..1226)
                     /locus_tag="VD788_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HXV75787.1"
                     /translation="MKGRAVVVLSLVAALAAAIASTAGAGSSSVAATQAVNCNGTLKI
                     GIVTPLTGGAGFLGQEQLSWTRFAISRLSKALGLKVQLVRGDTPVEKGPGEGLVVAQK
                     MIADSRLVAVIGPATSGAAASASKALFAAGIAHISPSATRTSLTYGSEKEGTPAFFRV
                     VPGDNIQGPTDANFMADKLNAKKVVIIDFQEPYSVGLANAAQAVLRRKNVEVIRQSTS
                     VNTTDFSSLVTRVPNDADIVFFPTQQPPDAQTFGQQLLEQGKRAKVFGGDGANSAAQF
                     KLAGSYVSNFAPDISGIASSKALIDGWQRANPNAELGSFGPPAYLATQVALQAIKRAC
                     VAGKGKLNTRRDVVRQVKKVRVPNSILGGTFRFSTKTNDPLNGSFYIFEVQSDKTYKL
                     VT"
     gene            1525..2514
                     /locus_tag="VD788_05660"
     CDS             1525..2514
                     /locus_tag="VD788_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV75788.1"
                     /translation="MPAVVEAIDLHRTYKTTTGTFRRRSLEVEAVRGVSFEIAEGELF
                     GLLGPNGAGKTTTIKMLITLLIPTSGKARVLGHDVVEDAHWVRERIGYVFGGDRGLYE
                     RLSALDNLRYFAELYGVDPKHQRARIGELLELVGLEGREKERVEGYSRGMRQRLHIAR
                     GLLHDPPVIFLDEPTIGVDPVGARELRATISSLKAAGKTVLLTTHYMFEADSLCDRIA
                     VVAKGEIVAAGTPRDLKARVSDGVVVEVEVYGIEDSAVEGLRGIDGVTSVAVEDRDQA
                     QVVVVQAMEGHEPTHAILARLQGVSLGRVTTREPTLEDAYVALVTEQPVAADG"
     gene            2507..>3134
                     /locus_tag="VD788_05665"
     CDS             2507..>3134
                     /locus_tag="VD788_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV75789.1"
                     /translation="MAEAGRPGVLRLGRVLAIGFRLHLKMMTRSSFDGFLNVLWPLFF
                     ATVAFFMFQAGAGGETLVFASFGAAVMGIWSSTSTSAGSAMQRERWHGTLELLVCAPV
                     HFAAVLLPVTVAMSAIGLYSMVATLLVSRFAFGVDLPFEQPVLFAVALVATVASIGAL
                     GFLLAVSFVRYRTAWALGNLLEYPVWLVAGFLVPLALLPGWVRPISYVL"
BASE COUNT          421 a         1098 c         1092 g          523 t
ORIGIN      
        1 cccctggacg ccgcttcgag gctgcgtccg gggagggagc tctaggtcac gagcttgtac
       61 gtcttgtcgg actgcacctc gaagatgtag aacgatccgt tgagcgggtc gttcgtcttc
      121 gtcgagaagc ggaacgtgcc accgaggatc gagttcggca cgcgcacctt cttgacctga
      181 cgcacgacgt cgcgacgcgt gttcagcttg cccttgccgg cgacgcacgc ccgcttgatc
      241 gcctgcagcg cgacctgcgt cgcgaggtag gcgggcgggc cgaacgagcc gagctccgcg
      301 ttcgggttcg ccctctgcca cccgtcgatc agcgccttcg acgaggcgat gccgctgatg
      361 tccggggcga agttggagac gtacgagccg gcgagcttga actgcgccgc gctgttcgca
      421 ccgtcaccgc cgaacacctt cgcccgcttg ccctgctcga gcagctgctg gccgaaggtc
      481 tgcgcgtccg gcggctgctg ggtcgggaag aagacgatgt ccgcgtcgtt cggcacgcgc
      541 gtgacgagcg acgagaagtc ggtcgtgttg accgacgtcg actggcggat cacctcgacg
      601 ttcttgcgcc gcaggaccgc ctgcgcggcg ttcgcgaggc cgaccgagta cggctcctgg
      661 aagtcgatga tcacgacctt cttggcgttg agcttgtccg ccatgaagtt cgcgtcggtc
      721 gggccctgga tgttgtcacc cggcacgacg cggaagaacg ccggcgtgcc ctccttctcg
      781 ctcccgtagg tcagcgaggt gcgggtcgcg gacggcgaga tgtgggcgat gccggcggcg
      841 aacagcgctt tgctggccga cgcggcggca cccgacgtcg ccggtccgat caccgccacg
      901 aggcgcgagt cggcgatcat cttctgggcg acgacgagac cctcgcccgg ccccttctcg
      961 accggcgtgt cgccccgcac gagctggacc ttcaggccga gcgccttcga gagccgcgag
     1021 atcgcgaacc gcgtccacga cagctgctcc tggccgagga agccggcgcc gccggtcagc
     1081 ggcgtgacga tgccgatctt cagcgtgccg ttgcagttga ccgcctgggt cgcggcgacg
     1141 gacgacgagc ccgcccccgc agtcgatgcg atcgcggccg ccagggcggc gaccagcgac
     1201 agcaccacta ctgcgcgtcc cttcattcct gtcctccttg tgggatcccc atgtgagacg
     1261 tcgtcctccg tccgttgttg cacgcgacgg cgcgcgtcgc accgcagagc gacgtcaact
     1321 gtcgcagcag accctctgac atgtcaaccg cttgcctgcg agcgcgggat cccgtgggat
     1381 cccgcgccgc aacatgcaaa tccctgaagt cgcctgccgc ctacggcggc ggcggaggcg
     1441 ccgaccgtgc cgccggtagc ttcgtcgcga cagtggtgtc gaccgtacgt ctccggcaac
     1501 gccgaggggc taacgtgcgc gcccgtgccc gccgtcgtcg aagcgatcga cctccatcgg
     1561 acctacaaga cgacgaccgg caccttccgc cgccgctcgc tcgaggtcga ggcggtccgc
     1621 ggcgtcagct tcgagatcgc cgagggcgag ctcttcgggc tgctggggcc gaacggcgcg
     1681 ggcaagacga cgacgatcaa gatgctgatc acgctcctga tccccacgtc ggggaaggcg
     1741 cgcgtcctcg gccatgacgt cgtcgaggat gcacactggg tgcgcgagcg gatcggctac
     1801 gtgttcggcg gcgaccgcgg cctctacgag cgcctctcgg cgctcgacaa cctgcgctat
     1861 ttcgcggagc tgtacggcgt cgacccgaag caccagcgcg cccggatcgg ggagctgctc
     1921 gagctcgtcg gcctcgaggg ccgcgagaag gagcgcgtcg agggctactc ccggggcatg
     1981 cggcagcggc tccacatcgc gcgcgggctc ctccacgatc caccggtgat cttcctcgac
     2041 gagccgacga tcggcgtcga cccggtcggc gcgcgcgagc tccgcgcgac gatctcgagc
     2101 ctcaaggcgg ccgggaagac ggtgctcctg acgacgcact acatgttcga ggcggactcg
     2161 ctctgcgacc gcatcgccgt cgtcgcgaag ggggagatcg tcgccgcggg cacgccgcgc
     2221 gacctgaagg cgcgtgtctc ggacggcgtc gtcgtcgagg tcgaggtcta cgggatcgag
     2281 gacagcgccg tcgaagggct tcgcgggatc gacggcgtga cgtcggtcgc ggtcgaggat
     2341 cgcgaccagg cgcaggtcgt cgtcgtccag gcgatggagg ggcacgagcc gacgcacgcg
     2401 atcctcgcgc ggctgcaggg ggtctcgctc gggcgcgtga cgacgcgcga gccgacgctc
     2461 gaggacgcgt acgtggcgct cgtcaccgag cagccggtcg cggccgatgg ctgaggccgg
     2521 ccggcccggc gtcctccggc tcggccgcgt ccttgcgatc gggttccggc tccacctgaa
     2581 gatgatgacc cgctcgtcgt tcgacggctt cctgaacgtc ctctggccac tcttcttcgc
     2641 gaccgtcgcg ttcttcatgt ttcaggcggg cgccggcggg gagacgctcg tgttcgcctc
     2701 gttcggggcg gcggtcatgg gcatctggtc gtccacgagc acgtcggccg ggtcggcgat
     2761 gcagcgcgag cgctggcacg ggacgctcga gctgctcgtc tgcgcgcccg tccatttcgc
     2821 cgcggtgctg ctgcccgtga ccgtcgcgat gtcggcgatc gggctctaca gcatggtcgc
     2881 gaccctgctc gtgagccgct tcgcgttcgg cgtcgacctc ccgttcgagc agcccgtgct
     2941 gttcgcggtc gcgctcgtcg cgacggtcgc ctcgatcggc gcgctcggct tcctgctcgc
     3001 cgtctcgttc gtccgctacc gcaccgcctg ggcgctcggg aacctcctcg agtacccggt
     3061 ctggctcgtc gccggcttcc tcgtgccgct cgccctcctt cccggatggg tccggccgat
     3121 ctcgtacgtg ctag
//
LOCUS       DASPGN010000200         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1556079, whole genome shotgun sequence.
ACCESSION   DASPGN010000200 DASPGN010000000
VERSION     DASPGN010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556079"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VD788_05670"
     CDS             <1..632
                     /locus_tag="VD788_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75790.1"
                     /translation="IGGVTGARFFHPAHPDQEGVDQVVRNNFIADCDDAVFEIRGALR
                     ARIYHNTVVTQTRFAIFRFQTGNSAADVPSGNDEIEIANNLIVSAGGDPQYARNDAGE
                     GAFVFRSQLWAGPFHNSGATGPAMPRFPLATDTVVSNHALAPVLARATFDGLSGLADA
                     FERYRPAVGSPAVRAGAANSVAIVDAGGRARDVARPSIGALEPDSRSHD"
     gene            748..2715
                     /gene="asnB"
                     /locus_tag="VD788_05675"
     CDS             748..2715
                     /gene="asnB"
                     /locus_tag="VD788_05675"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXV75791.1"
                     /translation="MCGIAGMFNRDGRPVDADLLVEMTRTLVHRGPDEEGYYLNRAVD
                     GLAERALAPFSGSYGSGNVGLGHRRLSIIDLASGQQPLSNEDGTVWVSFNGEIYNFET
                     LHAELEAKGHRFRTRCDTETIVHAYEEWGERCVERFRGMFAFAVWDDNRRQLYLARDR
                     VGKKPLYYLLDADRFIFGSEMKAILRAPGVPREIDPTALSDFLSLLYVPSPKSILSAV
                     KKLPAAHYAVVTADTFRSQPYWDLPFRPISDESEELVIEGILSGIEEATRIRMISEVP
                     LGAFLSGGVDSSAVVAMMAKSSTEPVVTNAISFSVAKYDEAVHARRVADLFATNHHEY
                     HVTPEAIPVIEQLAWHYDEPFADSSAVPTYYVSKTARERVTVSLSGDGGDESFAGYRR
                     YSFDMFENRVRNLVPRALGRPLFSLLGSLYPKADYLPQVFRGKAFISNLARDPVDAYF
                     YSVSAFKEETKAALLAPEVSETLGGYRTQDWFREIYDRAPADDHLSRLQYLDIKTYLC
                     EDILTKVDRASMAVSLEVRCPLLDHVVLERVARIPWQMKLVGTNGKHVFKRALKRHLP
                     DDILYRRKMGFGVPIVEWLRTACREYAAARILEGEGSRRYFSRDRLDKMWKDHQRGVR
                     NWSTELWIVLMFNLWHERFATNVGAEPRPRA"
     gene            2684..3802
                     /locus_tag="VD788_05680"
     CDS             2684..3802
                     /locus_tag="VD788_05680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV75792.1"
                     /translation="MWELSLDRAREDGRLEVDWLDLQARAHGSYFTSWGWVGTWLRTL
                     PAEIAIHALRVRRDGRLVGLALLGHRATVRRKIIRSTVLLVSETGLPAYDTLTVEHNE
                     LLAEAGLEPEVAGRVVAWLCEASVPWHELRIAGVDQSRSQPYLDAASACGLKAFVEAD
                     KPYFYVDLRQVRGGGGDYLSTVSGNTRYQIRRALREYERRGEVSIRPAADVDHALELL
                     GSLKELHQRHWVDRGERGAFGTDFALSFHDSLVRARFPHGEIQLLRVSVGDRPVGYLY
                     NLVQGGVVSNYQSAFVYEDDPKVKPGLVSHYKAIELNLARGADCYDFLMGHQRYKSSL
                     ATDEERMIWLVLQRRLMRFELEHRLRQAWRRWTARAGG"
BASE COUNT          612 a         1279 c         1277 g          634 t
ORIGIN      
        1 agatcggagg agtcaccggc gcgcggttct ttcatcccgc tcatcccgat caggagggtg
       61 tcgatcaggt cgtccgcaac aacttcatcg ccgactgcga cgacgcggtg ttcgagattc
      121 ggggcgcgct ccgcgcgcgc atctaccaca ataccgtcgt gacccagacg cgcttcgcga
      181 tcttccgctt ccaaaccggc aactcggcgg ccgacgtacc gagcggcaac gacgagatcg
      241 agatcgcgaa caatctgatc gtcagcgccg gcggcgatcc gcagtacgcg cggaacgacg
      301 ccggcgaggg tgcgttcgtc ttccgctcgc aactgtgggc ggggccgttc cacaactccg
      361 gagcgaccgg gccggcgatg ccgcggttcc cgctggcgac cgataccgtc gtctcgaacc
      421 acgcgctcgc gccggtcctc gcgcgagcga cgttcgacgg cctctccggg ctcgccgatg
      481 cgttcgaacg ctaccgcccg gcggtcggca gtccggccgt gcgggccggg gcggccaact
      541 cggtcgcgat cgtcgacgcc ggcggtcgcg cccgggacgt ggcgcgaccc tcgatcgggg
      601 cgctcgagcc cgactcgagg tctcacgact gactggacag ggcgcggacc cccgatatat
      661 tctctgtcgc tgaccctcga gtgcttgtca gaccacgttt cccacccacc tcgggctgcg
      721 ctcgtccccg gaggccgtag agcgacgatg tgcgggatcg ccggcatgtt caaccgggac
      781 ggtcgaccgg tcgacgccga cctgctcgtc gagatgacgc gcacgctcgt gcaccgcggc
      841 ccggacgagg agggttacta cctcaaccgc gccgtcgacg gtctcgccga acgggcctta
      901 gcgcccttct cgggatcgta cggctccggc aacgtcggcc tcggccaccg gcgcctgagc
      961 atcatcgacc tcgcctccgg ccagcagccg ttgagcaacg aagacgggac ggtctgggtc
     1021 tcgttcaacg gcgagatcta caacttcgaa acgttgcacg ccgagctcga ggcgaagggg
     1081 caccgcttcc gcacgcgctg cgacaccgag acgatcgtcc acgcctacga agaatggggc
     1141 gagcgctgcg tcgaacggtt ccgcggcatg ttcgcattcg ccgtctggga cgacaaccgg
     1201 cgccagctct acctggcgcg ggaccgcgtg ggaaagaagc cgctctacta cctgctcgac
     1261 gccgaccggt tcatcttcgg ctccgagatg aaggcgatcc tccgggcgcc gggggtgcca
     1321 cgcgagatcg acccgaccgc gttgtcggat ttcctgtcgc tgctctacgt tccgtcaccc
     1381 aagtcgatcc tgtcggccgt caagaaactg ccggccgcgc actatgcggt cgtgaccgcg
     1441 gacacctttc gcagccagcc gtactgggac ctgccgttcc ggccgatctc cgacgagtcg
     1501 gaagagctcg tgatcgaagg aatcctgtcc gggatcgagg aggcgacgcg catccggatg
     1561 atcagcgagg tcccgctcgg cgccttcctg tcgggcggcg tcgactcgtc cgcggtcgtc
     1621 gcgatgatgg ccaagtcgtc gaccgagccc gtcgtcacca acgcgatctc gttcagcgtg
     1681 gcgaagtacg acgaagcggt gcacgcgcga cgggtggccg acctgttcgc caccaatcac
     1741 cacgagtacc acgtcacgcc cgaggcgatc ccggtgatcg agcagctggc ctggcactac
     1801 gacgagccgt tcgccgactc gtccgccgtg ccgacctact acgtgtcgaa gaccgcccgc
     1861 gaacgcgtga cggtctcgct cagcggggac ggtggcgacg agagcttcgc gggctaccgg
     1921 cgctacagct tcgacatgtt cgagaaccgc gtacggaacc tcgttccccg tgctctcggc
     1981 cggccgttgt tctcgttgct cggcagcctg tatcccaagg cggactatct cccgcaggtc
     2041 tttcgcggca aggccttcat ctcgaacctc gctcgagatc ccgtcgacgc ctacttctat
     2101 tcggtcagcg cgttcaagga agagaccaag gcggcgctgc tcgcgccgga ggtgagcgag
     2161 accctcggcg gctaccgcac gcaagactgg ttccgcgaga tctacgaccg ggcgccggcc
     2221 gacgaccatc tctcgcgcct ccagtacctc gacatcaaga cctacctctg cgaggacatc
     2281 ctgaccaagg tcgaccgcgc gagcatggcg gtctcgctcg aggtgcgctg cccgctgttg
     2341 gatcacgtcg tgctggaacg ggtggcccgc atcccgtggc agatgaaact ggtcgggacc
     2401 aacgggaagc acgtgttcaa gcgggcgttg aagcgccacc ttcccgacga cattctctat
     2461 cggcgcaaga tgggcttcgg cgtgccgatc gtcgagtggc tgcgcaccgc gtgccgggag
     2521 tacgccgcgg cgaggatcct cgagggcgag gggtcgcgcc gctacttttc gcgcgacagg
     2581 ctcgacaaga tgtggaagga ccatcagcgg ggcgtgcgca actggtcgac cgagctgtgg
     2641 atcgtgttga tgttcaatct gtggcacgag aggttcgcga cgaatgtggg agctgagcct
     2701 cgaccgcgcg cgtgaagacg ggcgcttgga ggtcgattgg ctcgacctcc aagcgagagc
     2761 ccacggttcc tacttcactt cttggggctg ggtcggaacg tggctgcgaa cgctgccggc
     2821 cgagatcgcc atccacgcgc tgcgtgtccg acgtgacgga cgcctcgtcg gcctggcgct
     2881 gctcgggcac cgggcgacgg tgcgccggaa gatcatccga tcgaccgtcc tgctcgtctc
     2941 ggaaaccggt ctgccggcct acgacacgct gaccgtcgaa cacaacgagt tgctggcgga
     3001 ggccggtctc gagccggagg tggccggtcg tgtcgtcgcc tggctgtgcg aggcgagcgt
     3061 gccgtggcac gagctgcgga tcgcgggtgt cgaccagtcc cgctcgcagc cgtatctcga
     3121 cgcggcgtcg gcctgcgggt tgaaggcgtt cgtcgaagcg gacaagccgt acttctacgt
     3181 cgatctgcgg caggtgcgcg gcggcggcgg cgactacctg tccacggtca gcggcaacac
     3241 acgctaccag atccgccgcg cgctccgcga gtacgaacgg cggggggagg tatcgatccg
     3301 tcccgcggct gacgtcgacc atgcgttgga gcttctcggc agcctgaagg agctgcacca
     3361 gcgacactgg gtcgaccggg gcgagcgcgg tgcgttcggc acggacttcg cgctgtcgtt
     3421 tcacgactcg ctggtccgcg cccgcttccc gcacggcgag atccagctgc ttcgtgtttc
     3481 ggtcggcgac cggccggtcg gttacctgta caacctggtc cagggcggcg tggtgtcgaa
     3541 ctaccagagc gcgttcgtct acgaggacga cccgaaggtc aagccgggcc tggtcagcca
     3601 ctacaaggcg atcgagctca atctggcgcg cggagcggat tgctacgact tcctgatggg
     3661 ccaccaacgg tacaagagta gcctggcgac ggacgaggag cggatgatct ggctcgtgct
     3721 gcagcgacgg ctgatgcgct tcgagctcga gcaccgcctg cggcaggcgt ggcgtcgctg
     3781 gaccgcccga gcgggcggct ga
//
LOCUS       DASPGN010000201         8660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_737109, whole genome shotgun sequence.
ACCESSION   DASPGN010000201 DASPGN010000000
VERSION     DASPGN010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8660
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737109"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VD788_05685"
     CDS             <1..419
                     /locus_tag="VD788_05685"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HXV75793.1"
                     /translation="LFLVLYLLTTIYTLIEVPRATANFARFPWAAAVVLINVLAIANV
                     PRALFTGRYGQAFVCSSLTIAAMVALFALALFPNILPASNDPTHSLTMYNAASSTMTL
                     KIGLLFVAIGLPFILAYTAVIYWTFRGKVRIGEHSY"
     gene            complement(627..1907)
                     /locus_tag="VD788_05690"
     CDS             complement(627..1907)
                     /locus_tag="VD788_05690"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXV75794.1"
                     /translation="MGLSGNLNTMSVPELLGWVDVGRKSGTLEVERDKIIKRLIFRDG
                     RLVACSSNDPATLMGHFLLARGAISRELLSQALARQEQSEENLGELLLEMGVITQSHR
                     DAFVVAKIEETIYGLFDWPDAAFRFHPGALEDANALEVELEIRDVIETGMRRMVEREL
                     LAEVINDQGIVLRRLGEGEPPEIAGSQTARRIFALIDGVKTVKQILLHSHAPEFIVTK
                     FLTALVHSGSAEVAEVRCDPPARELAEKEAADEWLDAMVETVEAEPPREAGDGARDES
                     QTSPGARREYRPLADRTTQDSRELQGEINVALQLMSGGQPEAALELLNAMAAAHPGDV
                     SLRQLVVNAEQDFCDKTLAGELRPDYVPTRASAAAALAAGRLSAEESFLLEQIDGASD
                     VRSLLWVSPLREVDALKTLRRMLNKGWIEIRRAA"
     gene            complement(1957..3480)
                     /locus_tag="VD788_05695"
     CDS             complement(1957..3480)
                     /locus_tag="VD788_05695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75795.1"
                     /translation="MTTPTKRGTGIGAGRFPWIVGLLALAALPYLAALDQPLLRDDRT
                     LLDNEWLIDQASVVNVFQHHYWHGSRHDRSDLYRPLTILTLAWNLRGAAGAAGLRVVN
                     VLLHALACVLVWWVLRCVFGGSIREREVADRVAWTAAAWFAVHPLASEGVWFVTGRAE
                     LLAASCGLLAFGCLVDPRLARRETAAVVCSTVAFALALGFKESAASWIAILGLWYLVL
                     RATARPARVVVRTLVAWGVVLGLFLFVRASAVGWLPHEPPWIDNPLVRVGAPTRVANA
                     VLIGGLYLARMALPLRLSVEYGYDQIGVVPLLPWGGIAAACAVVAWCALAATLYRRSP
                     VAAFVWAFVPASFAVTGNVAFPIGTMFAERLAYLPLVGFCGLAAWFVWRLPVTRFTVW
                     ALVVLVLVAGAARGFARTRDFRDVDTFHQATALASPRAVKALANLGRTRLRQGRLDEA
                     VAVLERAVAIWPDYSGALSLLADVHARAGDPAAEAYYRRRAREAYARWRSGEDAPSF"
     gene            complement(3699..4268)
                     /locus_tag="VD788_05700"
     CDS             complement(3699..4268)
                     /locus_tag="VD788_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75796.1"
                     /translation="MRNAIGVSSVLWLALVGCAPPEAPPGDTTPAEPGSTELLPKPFT
                     SEQIRDEMGLGLSIVLRHTTADEVALHRWTVVAADDEGVEIEYATIDESGQPLGEPRR
                     EWSGWDELRDHATFFGRTTTRERVELATPLGTLAGWLYRTEESDPGSIRETFFADALP
                     GAPVFARTTEGGRVVAEVVQLERSRAGDR"
     gene            4350..5555
                     /locus_tag="VD788_05705"
     CDS             4350..5555
                     /locus_tag="VD788_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXV75797.1"
                     /translation="MIPVEHHRAALVPAEEIARRITGFQRRLQGAAIDVAWIDHLTDR
                     LYLSGSAQSGVLLVPASGPPVFHVRKSHARATAEARVAVRPYAGRAELFAALGGLARG
                     EGRVGLALDVTPAATLLAITERTGLAVADVTAPIRLERARKSPWELEQLAGAARQITT
                     LLDGIADAIRPGVSELALTGTVEGRLRAAGHGGTVRVRRPGTDIAIATVVSGTSAFYP
                     TAFDGPVGAPGPTPPTPAGGGFKPLVAGETVMLDVVSSYNGYHVDTTRCFFLGRNPPD
                     VVLRAHDRCRELLRRVVAALRPGARCDAIYREATAWAAAAGEPPGFMGYGENRVRFLG
                     HGVGLELDELPVLADKIETRLEPGMVVAVEPKAFVEPYGPVGVENTYVVTDGEARNLC
                     PLADDLIAL"
     gene            5671..6990
                     /locus_tag="VD788_05710"
     CDS             5671..6990
                     /locus_tag="VD788_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75798.1"
                     /translation="MKRLIDSIAPRGLIPALVACLAIVAVPLVEAGSLQDFERRERPS
                     SGAKITTPPVGRDCDAEFTPDKHNDRIDCKTENANAALYDYIDTVVEFDMMRRNMGRE
                     PIFTDSQMDQLMTARERAQAAKNRSHEAETFRGAVKKQKAEDEDCYTKEIIGDNRGDD
                     VQPCEAGEDCEELLDDGIGNDDGKCMLKGNNREVCVQVCQQPLLSDMDTYDADQAGDF
                     EQGLEEIETALVDATEETKRAMARMRDAYDASRGTANECDMFQFDLRPTAAARQVAQV
                     AKNATGATFNGCSVVCNQDAFGWNCEAACLVFAIIDGIANAINDALDVADSNNGSAQL
                     DRVARCTTQLDSEIQEISADVDGTAAALDDVNERLDGVTDRLAALTEQIDALSAMMVQ
                     RFNAVDGYLCVPQGQRECFPGGQHDASRSGGGFSIERSGGESLPGRR"
     gene            complement(7009..7278)
                     /locus_tag="VD788_05715"
     CDS             complement(7009..7278)
                     /locus_tag="VD788_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXV75799.1"
                     /translation="MGKKLFVGGISWNTTEDGLREAFERFGEVVEANIVFDRYTGRSR
                     GFGFVTFANEEQAAQAMEQLNGAELDGRAIRVDDAHDKPKAGGPR"
     gene            complement(7391..7837)
                     /locus_tag="VD788_05720"
     CDS             complement(7391..7837)
                     /locus_tag="VD788_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75800.1"
                     /translation="MLRLSPDESIPHHFEAVPLRRPFVAVGWTAALLSCAGGLWLAAT
                     AYGPSAEILAPFLVVAGALLAVAVVRCRRHELTVGTKRTELKVGPFERIVPTAAVTRV
                     SSRPAAGWRCLFSDREIVFEVEVGRRSVSLPSDDPDAVARAIDELK"
     gene            7933..>8660
                     /locus_tag="VD788_05725"
     CDS             7933..>8660
                     /locus_tag="VD788_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXV75801.1"
                     /translation="MKVNRPTLRSQLLFAGIALGVLVSAGYADAASTLTHHFDSETLD
                     LRHLIGEVRVEQHGGSGFEVEVQVAGRDARDGMIELRTEANRLDVLFPGGHSDFIYPA
                     LGRGSTSSIEIGHDKGELFHGPGGAGRLRVRGQGDGLELWADVVVRVPSGGRLEVDHA
                     IGAIRVEGVDAELELATDSGRIEVHDSSGEIAAATGSGEIELVRTDGKHVGLATGSGT
                     IRIESSSGDTYEVGAGSGNVSISGL"
BASE COUNT         1415 a         3033 c         2956 g         1256 t
ORIGIN      
        1 gcctgttcct cgtgctctat ctgttgacca cgatctacac gctgatcgag gtgccgcggg
       61 cgaccgccaa cttcgcgcgt ttcccgtggg ccgccgcggt cgtgctgatc aacgtcctcg
      121 cgatcgccaa cgtcccgcgc gcgctgttca ccggtcgcta cggtcaggcc ttcgtctgct
      181 cgtcgctgac gatcgcggcg atggtcgcgt tgttcgcgct ggcgctgttc ccgaacatcc
      241 tgccggcgag caacgatccg acccacagcc tgacgatgta caacgccgcg tcgagcacga
      301 tgacactgaa gatcggcctg ctgttcgtcg cgatcggcct gccgttcatc ctggcctaca
      361 ccgcggtgat ctactggacg tttcgcggaa aggtgcggat cggcgagcac agctactgaa
      421 ccgcgcgtcg ctccaaatac ctggcgtggg tgtgctgtgc gtacgcagcg cggttcatct
      481 cggctgtcgg atcgagcccg gccgtcaccg taccctttct ccaggggagc agtcgcagtg
      541 aacgggctga aacctttaat cggttaacga tggcacccct gagggaacca ggagtcttgc
      601 ttctgctccc cactccccgc gccgcctcag gcggcccgac ggatttcgat ccagcctttg
      661 ttcagcatac gtcgcagggt cttgagcgcg tcgacctctc gcaacggtga cacccacaac
      721 agcgacctga cgtcggacgc accgtcgatc tgctcgagga ggaacgactc ctcggccgac
      781 agccgccccg cggccagcgc cgccgcggcg gaggctcggg tcggcacgta gtccggtcgc
      841 agctcgccgg ccagtgtctt gtcgcagaag tcctgttccg cgttgacgac caactgtcgc
      901 agtgagacgt cgcccgggtg cgcggcggcc atcgcgttca acaactcgag tgcggcctcg
      961 ggctgccccc ccgacatcag ctgcagcgcg acgttgatct cgccctgaag ctcgcgtgag
     1021 tcctgcgtcg ttcggtcggc cagcggtcga tactcgcgcc tcgcacccgg tgacgtctgc
     1081 gattcgtcgc gtgcgccgtc acccgcttcc cgcggaggtt ccgcctcgac cgtctcgacc
     1141 atcgcgtcga gccattcgtc ggccgcctcc ttttcggcga gctcccgcgc cggcggatcg
     1201 caacgcacct cggcgacctc ggcgctccca ctgtgcacga gcgcggtcag gaacttggtg
     1261 acgatgaact ccggcgcgtg gctgtgcagc agaatctgct tgacggtctt gacgccgtcg
     1321 atcagcgcga agatgcgtcg tgccgtctga cttcccgcga tctccggcgg ctcgccttcg
     1381 ccgagacgtc gcagcacgat cccttggtcg ttgatcacct cggccaacaa ctcgcgctcg
     1441 accatccggc gcatgccggt ttcgatgacg tcgcgaatct cgagctcgac ctcgagcgca
     1501 ttggcgtctt ccagtgcgcc gggatgaaag cgaaaggcgg cgtccggcca gtcgaacagg
     1561 ccgtagatcg tctcctcgat cttggccacg acgaacgcgt cgcggtggga ctgtgtgatc
     1621 acgcccatct cgagcaacag ctcgcccagg ttctcctcgc tctgctcttg ccgagcgaga
     1681 gcctggctga gcagctcgcg ggagatcgcg ccgcgggcca gcagaaagtg gcccatcagc
     1741 gtcgccgggt cgttcgacga gcaggcgacc agccggccgt cgcggaagat caggcgtttg
     1801 atgatcttgt cgcgctcgac ctcgagcgtt ccgctcttgc ggccgacgtc gacccagccg
     1861 agcaactcgg ggaccgacat cgtgttcagg ttgccgctga gccccatacc ccgacctccc
     1921 gatccagagt ggaaggtatg tccccgtacg cgattgtcaa aaggacgggg cgtcctcgcc
     1981 cgaccgccaa cgcgcgtacg cctcgcgcgc tcgccggcga tagtaggcct cggcggccgg
     2041 gtccccggcg cgggcgtgga cgtcggccaa cagcgacaac gctcccgaat aatcgggcca
     2101 gatcgccacc gcccgctcga gcacagcgac cgcctcgtcg aggcgcccct ggcgcaggcg
     2161 ggtgcgcccg aggttggcca gcgctttgac cgcgcggggc gacgccagcg cggtcgcttg
     2221 gtggaacgtg tcgacgtcgc gaaagtcgcg cgtgcgcgcg aatccccggg cggcgcccgc
     2281 gacgagcacc agcacgacca gcgcccagac ggtgaagcgg gtgaccggca ggcgccagac
     2341 gaaccaagcg gcgagcccgc agaaaccgac gagcggcagg tatgccagcc gctcggcgaa
     2401 catcgtcccg atcggaaagg ccacgttgcc cgtgacggcg aacgacgcgg gcacgaaggc
     2461 ccagacgaag gcggcgaccg gcgagcggcg gtagagcgtc gccgccaacg cgcaccaggc
     2521 gacgacggcg caagccgcgg cgatcccgcc ccacggcagc agcggcacga cgccgatctg
     2581 gtcgtagccg tactcgacgc tcagccgcag cggcagggcc atgcgcgcca gatacaagcc
     2641 accgatcagc acggcgttgg cgacgcgggt cggcgctccg acccgcacca gcgggttgtc
     2701 gatccacggc ggctcgtgcg gtagccagcc gaccgccgac gcgcggacga acaggaacag
     2761 gccgagcacg acgccccacg cgacgagcgt ccgcacgacg acgcgggccg gcctcgccgt
     2821 ggcgcgcagg accaggtacc agaggccgag gatcgcgatc cacgacgcgg cgctctcctt
     2881 gaacccgagc gccagcgcga aggcgaccgt cgagcagacg acggccgccg tctcgcgccg
     2941 cgccagccgt gggtcgacca gacaaccgaa ggcgaggagg ccgcacgacg cggccagcag
     3001 ctcggcgcgg ccggtcacga accatacccc ctcgctggcg agcgggtgga ccgcgaacca
     3061 cgcggcggcg gtccaggcga cgcgatcggc cacttccctc tcgcggatcg acccgccgaa
     3121 cacgcaacgc agtacccacc acaccagcac gcaggcgagc gcgtgcagca ggacgttcac
     3181 cacgcgcaga ccggcggcgc cggcggcgcc ccgcaggttc caggcgagcg tgaggatcgt
     3241 cagcggccgg tacaggtccg atcgatcgtg tcggcttccg tgccagtagt gatgctggaa
     3301 cacgttgacg acggatgcct ggtcgatgag ccactcgttg tcgagcaggg ttcgatcgtc
     3361 gcgcagcagc ggctggtcga gcgccgcgag gtaaggcagc gccgccagcg ccaacagacc
     3421 gacgatccac gggaagcggc ccgcgccgat accggtgcct ctcttcgtcg gggtcgtcac
     3481 gatcggggcc ggattctaac agcgccggac ggagtgggcg aaagggccgt gacggggccg
     3541 ccttgcacgc gcggcccgat cacggtgggc gcaggcccgg gggaggttga gtacggtcgg
     3601 catggttgta cgtcgcgatc gcgggaccga caacgcgcgg cgtatcgttc gagcggacga
     3661 gcgatccgca gcggcgacgc ccagaccgac gtcgtggctc accggtcgcc ggctcgcgac
     3721 cgctcgagct gaaccacctc ggccacgacc cgaccccctt ccgtcgtgcg cgcgaagacc
     3781 ggcgcccccg gaagcgcgtc ggcgaagaag gtctcgcgga tcgagccggg gtccgactcc
     3841 tccgtgcgat agagccaacc ggcgagcgtg ccgagcgggg tcgcgagctc gacgcgttcg
     3901 cgcgtcgtcg tgcggccgaa gaaggtggcg tgatcgcgta gctcgtccca gccgctccac
     3961 tcgcggcgcg gttcgccgag cggctggccg gactcgtcga tcgtcgcgta ctcgatctcg
     4021 acaccctcgt cgtccgccgc gacgaccgtc cagcggtgga gcgcgacctc gtccgcggtc
     4081 gtgtggcgta gcacgatgct gaggcccagg cccatctcgt cgcggatctg ctccgaggtg
     4141 aacggcttcg gcagcagctc ggtcgaaccc ggctccgccg gcgtcgtgtc gccgggaggc
     4201 gcctcgggcg gcgcacagcc gacgagcgcg agccacagta ccgacgacac gccgatcgcg
     4261 ttccgcattg gtagatccta taccctcgga ggccacgccg cgattccgct gccgttgccg
     4321 atgccggcgg tatcgctcac aatgccgcga tgatcccggt cgagcatcac cgagcggcgc
     4381 tggttccggc cgaggagatc gcccggcgga tcaccggctt ccaacggcga ctgcaaggcg
     4441 ccgcgatcga cgtggcgtgg atcgaccatc tgaccgatcg gctctacctg tcgggatcgg
     4501 cccagagcgg cgtgctgctc gttccggctt cgggcccgcc ggtgttccac gtgcgcaaga
     4561 gccacgcgcg ggcgacggcc gaggcacggg tcgcggtgcg gccgtatgcc ggccgcgccg
     4621 agctgttcgc ggcgctcggc ggcctggcgc ggggcgaggg gcgcgtcggg ctcgcgctcg
     4681 acgtcacacc cgccgccacg ctgctcgcga tcaccgagcg gaccggtctc gccgtcgccg
     4741 acgtcacggc accgatccga ctcgagcgcg cccgcaagag tccgtgggag ctggagcagc
     4801 tcgccggcgc cgcacgccag atcaccacac tgctcgacgg gatcgccgac gccatccgtc
     4861 cgggcgtcag cgagctggcg ttgaccggga cggtcgaggg gcgcctgcgt gcggccggtc
     4921 acggcgggac ggtgcgcgtg cgccggcccg gcacggacat cgcgatcgcg accgtcgtgt
     4981 cgggaaccag cgcgttctat ccgaccgcgt tcgacggacc ggtcggtgca cccggcccga
     5041 cccccccgac cccggcgggc ggcggcttca agccgctcgt cgcgggcgag acggtgatgc
     5101 tcgacgtcgt cagctcctac aacggctacc acgtcgacac gacgcgctgc ttcttcctcg
     5161 gccgcaaccc gcccgacgtg gttcttcgag cccacgaccg ttgccgcgag ctgctgcgac
     5221 gcgtcgtcgc tgcgctgcgc cccggtgcac gctgcgatgc gatctaccgc gaagcgaccg
     5281 cgtgggccgc ggctgccggc gagccgcccg gcttcatggg ctacggcgag aaccgggtac
     5341 ggtttctcgg ccacggcgtc ggcctcgagc tggacgaact gcccgtgctg gcggacaaga
     5401 tcgagacgcg gctcgagccg ggtatggtcg tcgcggtcga gcccaaggcg ttcgtcgaac
     5461 cgtacggacc ggtgggcgtc gagaacacct acgtcgtcac cgacggcgag gcgaggaacc
     5521 tgtgcccgct ggccgacgat ctgatcgcgc tctagctagg tccggtcacg tgcggctccc
     5581 cgcgcgaaag cacgacttga tcgcctggcc gaatgcccgt acatgaaccg acacacgttc
     5641 gaaggttgtg gacaaaagta ggggggaacc atgaaacgac tcatcgactc gatcgcgccg
     5701 cgcgggctga ttcccgcgct cgtggcctgc ctggcgatcg tcgccgtccc gctggtcgag
     5761 gccggctcgc tacaggattt cgagcgccgc gaacgaccgt cgtccggcgc gaagatcacc
     5821 acgccgcccg tcggtcgcga ttgtgacgcg gagttcacgc cggacaagca caacgaccgc
     5881 atcgactgca agaccgaaaa cgccaacgct gcgctctacg actacatcga cacggtcgtc
     5941 gagttcgaca tgatgcgccg caacatgggt cgcgagccga tcttcaccga ctcgcagatg
     6001 gaccagttga tgaccgcccg cgagcgggcg caggccgcca agaaccgttc gcacgaggcg
     6061 gagaccttcc gcggcgcggt caagaaacaa aaggccgagg acgaagattg ctacacgaag
     6121 gagatcatcg gggacaaccg gggagacgac gtccaaccct gcgaggccgg cgaggactgc
     6181 gaagagctgc tcgacgacgg catcggcaac gatgacggca agtgcatgtt gaagggcaac
     6241 aaccgcgagg tctgcgtcca ggtctgccag cagccgctgc tgagcgacat ggacacctac
     6301 gacgcggacc aggcgggtga cttcgaacag ggtctggagg agatcgaaac ggcgctggtc
     6361 gacgcgaccg aggagacgaa acgggcgatg gcccgcatgc gggacgccta cgacgcatcc
     6421 cgcggaacgg ccaacgagtg cgacatgttc cagttcgacc tgcgcccgac cgcggccgcg
     6481 cggcaggtcg ctcaggtcgc caagaacgcc accggggcca ccttcaacgg atgctcggtg
     6541 gtttgcaacc aggacgcgtt cggctggaac tgcgaggcgg cctgcctcgt cttcgcgatc
     6601 atcgacggca tcgccaacgc gatcaacgac gcgctggatg tggcggacag caacaacggg
     6661 tcggcccagc tcgatcgcgt cgctcgatgc accacgcagc tcgactcgga gatccaggag
     6721 atctcggccg acgtcgacgg caccgcggcg gcgctcgacg acgtcaacga acgactggac
     6781 ggcgtgaccg accggctggc agcgctgacg gagcagatcg acgcgctctc cgcgatgatg
     6841 gtccagcggt tcaacgccgt cgacggctac ctctgcgtgc cgcaggggca gcgcgagtgc
     6901 ttcccgggcg gccagcacga cgcgagtcgt tccggcggtg gattctcgat cgagcgttcc
     6961 gggggagagt ccctgccggg acggcgctga cgcgggtgga gagcgggctc accgcgggcc
     7021 gcccgccttg ggtttgtcgt gggcgtcgtc gacgcggatc gcgcgtccgt cgagctcggc
     7081 gccgttcaac tgctccatcg cttgcgcggc ctgttcctcg ttggcgaagg tgacgaagcc
     7141 gaagccgcga gagcgtccgg tgtagcgatc gaagacgatg ttggcctcga ccacctcgcc
     7201 gaagcgctcg aacgcctccc gcagtccgtc ctcggtcgtg ttccagctga taccaccgac
     7261 gaaaagtttc ttgcccatct gccactcctc gcccggcccc gggcgcgtcg agcgaccgca
     7321 ccgggaggac gcccgcaggt tacagtaggg tgacaggcga aacaaccgtt ttcccttcag
     7381 gcacttccgg ctatttcagc tcgtcgatcg cccgcgcgac cgcatccgga tcgtcggacg
     7441 gcagcgaaac actgcgacgc ccgacctcga cctcgaagac gatctctcga tcggaaaata
     7501 agcaccgcca ccccgccgcc ggtcgagacg agacccgcgt caccgccgcg gtcgggacga
     7561 ttcgctcgaa cggccccact ttcaactcgg tccgtttggt gccgaccgtc aactcgtggc
     7621 ggcggcaacg gaccactgcg accgccagca gcgcgccggc gacgacgagg aacggtgcca
     7681 ggatctcggc cgacggtccg taggcggtcg ccgccagcca cagaccgccc gcgcagctga
     7741 gcagcgcggc cgtccagccg acggcgacga agggacggcg cagcggcacg gcctcgaaat
     7801 gatgcggaat cgactcgtcg ggcgaaaggc ggagcatctt ccgatcttac ccttccaacc
     7861 cccggcccgc ttccggcatc tgatggtcgt agccagattc gtccgcctcc gggcgaccga
     7921 caagggggat ccatgaaggt caatcgtccg acccttcgca gccagttgct cttcgccggg
     7981 atcgcgctcg gcgtcctcgt gtcggccggg tacgccgacg cggcctcgac gctcacgcat
     8041 cacttcgatt cggagacgct cgacctgcgc cacctgatcg gcgaggtgcg ggtcgagcag
     8101 cacggcggtt ccggcttcga ggtcgaggtg caggttgccg ggcgcgacgc ccgtgacggg
     8161 atgatcgagc ttcgcaccga agccaatcgg ctcgacgtgc tgtttcccgg ggggcattcc
     8221 gacttcatct acccggccct cggtcgcggc tcgaccagct cgatcgagat cggccacgac
     8281 aagggtgagc tgttccacgg cccggggggc gcagggcggc tgcgggttcg cggccaaggc
     8341 gacgggctcg agctgtgggc cgacgtcgtc gtgcgcgtgc cgtcgggcgg ccggctcgag
     8401 gtcgaccacg cgatcggcgc gatccgggtc gagggtgtcg acgccgagct tgagctcgcc
     8461 accgacagcg gacggatcga ggtgcacgac agctcgggcg agatcgcggc ggcgaccggc
     8521 agcggcgaga tcgagctggt acggaccgac ggcaagcacg tcggtctcgc caccggcagc
     8581 gggacgatcc gcatcgagtc gtcgagcggt gacacgtacg aggtcggcgc gggaagcggc
     8641 aacgtgtcga tctcgggact
//
LOCUS       DASPGN010000202         4573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_737129, whole genome shotgun sequence.
ACCESSION   DASPGN010000202 DASPGN010000000
VERSION     DASPGN010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4573
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737129"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VD788_05730"
     CDS             <1..426
                     /locus_tag="VD788_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75802.1"
                     /translation="FVYRLFETGRVEVVEPGVVRAALLDNRLIRDEGLSLTQIYVLSA
                     ELNVDLLISGDVADYVEGTGFGGIPFVGFTARAMDARRGVVSWTSISNNRGTDGAGLF
                     EIGGVRGGHALASEMIRAVIATALAPADSIAAKSAARGR"
     gene            451..2160
                     /locus_tag="VD788_05735"
     CDS             451..2160
                     /locus_tag="VD788_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXV75803.1"
                     /translation="MDSVYRSSKASRLARRVALCTVVSMASIAAPVPAESARVLARVN
                     GEPITIDDLYQELDNFHRTVQDPRQAVERPRVDELLDRLINARLIIQEARNIGLDELA
                     EYAELIDGYRRGALRRMVLDRAVGGVEADPERVEQIHELLVKRYRIRGVLIESKEAAE
                     QLERRARAGEDFERLVGELAEKLQVRPTDPEMLYRPDELFPEIVAELNKLEVGQFTPV
                     VAVRETYAFAQLLEVRMLDDPDARAIAEDEALRVAVEQEKRAFVESRIARHAKVDREL
                     LAGIDLGAGSANLAELKTDSRPIATIDGAEPITVGDLCTAFERQFFHGVEKVSGRDEL
                     TPRLERVLHELIGAQVTLREARALGLDQTDEFHRQVDHYANGVLFAIFVERVVEPEVE
                     VTQVDLERYRERHIDEFTTPEMLQLDALVFDGEPQARRALDRLRNGADFDWTRVNAAG
                     QSPAGGPAGRIPFNPQMPLTTAEMPDALRQAVADAQPGDYRFLAGSPSGPYYVIQVRN
                     VIASQSQPLAQIADRISKPVFAEKRQQAIDEWTRELREASEVERLVDDRQLAELVGFG
                     SAE"
     gene            2174..4531
                     /locus_tag="VD788_05740"
     CDS             2174..4531
                     /locus_tag="VD788_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75804.1"
                     /translation="MTRSTAAGSRRRWTFRLVALVGLPLVVLAALELGLRAVAFGYDA
                     AFVRTCTRNGVAEYCDNPDFSRSFFPRSLARPPLAFTFPADKAPTTLRVFLVGGSAAQ
                     GDPDPTFGPSRMLELMLAERYPLRRFEVVNAAVTAINSHVVRTIVRDLARFEPDLFIV
                     FVGNNEVVGPYGAGTVFAPMLSRPLIRASIAARRTRLGQLVSGLVDAGSAGSAEWRGM
                     EMFLGQPMPADDPRMAEVYRNFEANLRAIVDTALDAGAGVVLTTVPTNLVDCAPFASS
                     PSAALAPAERERADRLVEQSDRVLATDGAAAALEPLLAAERIDGADAELQYRIGRLRI
                     ALGQSDAARPNFVAARDLDLLRFRADSTINRRIRDVAEAVAGPLVALADAESRFAAVS
                     PHGLPGEELFLEHVHPTFAGNYLIALGWLEQIASVVPGLEPADSGKSLPDADELARRL
                     AYSDFDRRRVWKSVESRVERPPFTGQLDHLARLQRVADRTGRSTPIDWPAVLDTYDTA
                     IAARPGDPWLRFNLAGVLAEIGDHDRAAAELLVFLVAFPHDPAARERLCRAEIERGRF
                     DSALAECQRVIERIPHHTPPYYATAFALASLGRLDEAVSVYRRLLALEPDAAPEILNE
                     IGRIEMHRSRHDAAVAVYREALDAARGRLLPDVRFNYASALRASGDDAAADRAFAAAE
                     TDYRQAVELDPTAVAPLLALGRCLVQLGRYDAAIETFERAARLDPTRPEPYLAIVRAL
                     ERQGVAQRAADRARDGARVLEQAGRAAAAAALRREAARLERPPQD"
BASE COUNT          680 a         1559 c         1629 g          705 t
ORIGIN      
        1 ttcgtttatc gcttgttcga gaccggacgg gtcgaggtgg tcgagcccgg cgtcgtccgc
       61 gcggcgctgc tcgacaaccg cctgatccgg gacgaggggc tgtcgctgac ccagatctac
      121 gtactcagcg cggagttgaa cgtcgatctc ttgatctcgg gtgacgtcgc cgattacgtc
      181 gagggcaccg gcttcggcgg catcccgttc gtcgggttca ccgcgcgcgc gatggacgcc
      241 aggcgcggcg tcgtgagttg gacgtcgatc agcaacaacc gcggcaccga cggcgcgggc
      301 ctgttcgaga tcggcggtgt acgcggcggt cacgcactcg cgtcggagat gatccgggcg
      361 gtgatcgcca cggcgctcgc tcccgcagac tcgatcgccg ccaagtcggc cgcccgcgga
      421 cgataaggtg cgacgaaggg gaagaacacg atggactcgg tctaccgtag cagcaaggcg
      481 agccgactcg cgcgccgcgt cgcgctgtgc accgtcgtca gcatggcgtc gatcgccgcg
      541 cccgtcccgg ccgagtcggc gcgggtgctg gcccgcgtca acggcgaacc gatcacgatc
      601 gacgatctct atcaagagct cgacaacttc caccgcacgg tccaggaccc ccggcaggcc
      661 gtcgaacgcc cgcgagtcga cgagctgctc gaccgcttga tcaacgcgcg gctcatcatc
      721 caggaggcgc gcaacatcgg tctcgacgag ctggcggagt acgcggagct gatcgacggc
      781 taccgccgag gtgcgctgcg gcggatggta ctcgaccggg cggtgggcgg cgtcgaggcc
      841 gaccccgagc gcgtcgagca gatccacgag ctgttggtca agcgttatcg aatacgaggc
      901 gtgctgatcg aatcgaagga ggccgcggaa cagcttgagc gacgggctcg agccggggag
      961 gacttcgagc gtctcgtcgg cgagctggcc gagaagctgc aggtcagacc gaccgacccc
     1021 gagatgctct atcgccccga cgagctgttc ccggagatcg tcgccgaatt gaacaagctc
     1081 gaggtcggtc agttcacccc ggtcgtcgcg gtccgcgaga cctacgcttt cgcccagctg
     1141 ctcgaggtca ggatgctcga tgatccggac gctcgtgcga tcgcggaaga cgaggcactg
     1201 cgcgtagcgg tcgagcagga gaagcgcgca ttcgtcgaat cgcggatcgc gcgtcacgcc
     1261 aaggtcgacc gcgagctgct cgccggtatc gatctcggcg ccgggtcggc gaacctcgcc
     1321 gagttgaaaa cggattcgcg gccgatcgcg acgatcgacg gcgccgaacc gatcacggtc
     1381 ggggacctgt gtaccgcgtt cgagcgacag ttcttccatg gcgtggagaa agtgagcgga
     1441 cgggacgagc tgacgccgcg tctcgagcgc gtgctgcacg agttgatcgg ggcacaggtc
     1501 acgctgcgcg aagctcgcgc gctgggtctc gaccagacgg acgagttcca ccggcaggtc
     1561 gaccactacg ccaacggcgt actgttcgcg atcttcgtcg agcgcgtggt cgagcccgag
     1621 gtcgaggtga cgcaggtgga tctggaacgg taccgggaac ggcacatcga cgagttcacg
     1681 acgcccgaaa tgctgcagct cgacgcgctg gtcttcgacg gggagcccca ggcgcgtagg
     1741 gcgctcgacc gcttgcggaa cggcgcggac ttcgactgga cccgggtcaa cgccgccggg
     1801 cagtcgccgg ccgggggacc cgccggacgg attccattca atccgcagat gccgttgacc
     1861 accgccgaga tgccggatgc gctgcgtcag gccgtcgccg acgcgcagcc gggagactat
     1921 cgcttcctgg ccggctcacc gtcggggccg tactacgtga tccaggtgcg caacgtgatc
     1981 gcctcgcaga gccagcccct cgcgcaaatc gccgaccgca tctcgaaacc ggtcttcgcg
     2041 gagaagcggc agcaggcgat cgacgaatgg acccgcgagc tgcgcgaggc gtccgaagtc
     2101 gaacgcctgg tcgacgaccg acagctggcg gagctggtcg gcttcggttc agccgaatag
     2161 cagcgggacg tcggtgacgc ggtcgaccgc agccggcagc cggcgacgtt ggacctttcg
     2221 actggtggcg ctcgtcggtc taccgctcgt cgtgctggcg gcgttggagc tcgggctgcg
     2281 agcggtggcc ttcggctacg acgccgcttt cgtccggacg tgcacgcgga acggggtcgc
     2341 ggaatattgc gacaaccccg atttcagtcg cagcttcttc ccgcgctcgc tggctcgccc
     2401 gccgctggcg ttcaccttcc ccgccgacaa ggcgccgacc acgctgcggg tgttcctcgt
     2461 cggcggctcg gcggcccagg gcgaccccga cccgacgttc ggcccgtcgc gtatgctcga
     2521 gctgatgttg gccgagcgtt acccgctgcg gcgattcgag gtggtcaacg cggcggtgac
     2581 ggcgatcaac tcgcacgtcg tacgcaccat cgtgcgcgac ctcgcccgat tcgagcccga
     2641 tctgttcatc gtcttcgtcg gcaacaacga ggtcgtcggg ccgtatggcg ccggcacggt
     2701 gttcgcgccg atgctgtcgc ggccgttgat ccgcgcgtcg atcgcggcgc gtcgcacgcg
     2761 gctgggtcag ctcgtctcgg gtctggtcga cgccgggtcg gcggggtccg ccgagtggcg
     2821 tgggatggag atgttcctcg gtcagccgat gccggcggac gacccgcgga tggccgaggt
     2881 ctaccggaac ttcgaggcga atctgcgcgc gatcgtcgac accgcgctcg acgccggcgc
     2941 cggtgtggtg ctgacgaccg tgccgacgaa cctcgtcgat tgtgctccgt tcgcgtcgag
     3001 cccaagcgct gccctcgcgc cggccgagcg cgagcgcgcc gaccgcctgg tcgagcaaag
     3061 cgaccgcgtg ctcgcgacgg acggcgctgc ggctgcactc gagccgttgc tcgcggccga
     3121 gcggatcgac ggcgccgacg ccgaactgca ataccggatc ggccgcctgc gcatcgcgtt
     3181 gggccaatcc gatgcggcgc gcccgaactt cgtcgccgcg cgggatctcg acctgctgcg
     3241 ctttcgcgcc gacagcacga tcaatcggcg gattcgcgac gtggccgagg cggtcgccgg
     3301 gccgctcgtc gcgctggccg atgccgagag ccggttcgcc gccgtcagcc cgcacggcct
     3361 gcccggcgag gagttgttcc tcgagcacgt gcacccgacc ttcgccggaa actacctgat
     3421 cgcgctcggc tggctcgagc agatcgcctc ggtcgtgccc gggttggaac cggcggacag
     3481 cggcaaatcg ctgccggacg cggacgagtt ggcgcgtcgt ctggcctatt cggacttcga
     3541 ccggcgacga gtatggaaat cggtcgaatc gcgagtcgag cggccgccgt tcaccggtca
     3601 gctcgaccac cttgcccgtt tgcagcgggt cgccgacagg acggggcgct cgaccccgat
     3661 cgattggccg gcggtgctcg acacctacga cacggcgatc gcggcgcgac ccggcgatcc
     3721 gtggttgcgt ttcaacctgg ccggtgtgct cgccgagatc ggcgaccacg accgtgcggc
     3781 ggcggagctc ttggtcttcc tcgtcgcttt tccgcacgat ccggccgcgc gcgagcgact
     3841 gtgccgcgcg gagatcgagc gtggtcgctt cgacagcgcg ctggccgagt gccagcgggt
     3901 gatcgagcgc atcccgcacc acacgccacc ctactacgcc acggcgttcg cgctggccag
     3961 cctcggtcgc ttggacgagg cggtatcggt ctaccggcgc ctgctggcgc tcgagcccga
     4021 cgcggcaccg gagatcctca acgagatcgg tcgcatcgag atgcaccgat cgcgtcatga
     4081 cgcggccgtc gccgtctacc gcgaggcgct cgacgccgcg cggggtcgcc tgctgcccga
     4141 tgttcggttc aactacgcct cggcgctgcg cgcgtcgggg gacgacgccg cggccgatcg
     4201 agcgtttgcc gcggccgaga ccgactatcg acaggcggtc gagctcgacc cgaccgcggt
     4261 tgcgccgctg ttggccctcg gccgctgtct ggtccaactc ggacggtacg acgccgcgat
     4321 cgagaccttc gagcgagccg cccgactcga cccgacgcgg ccggagccgt acctcgcgat
     4381 cgtgcgggcg ctcgagcgac aaggggttgc ccagcgggcg gccgaccgag cgcgcgacgg
     4441 cgcccgcgtg ttggagcagg ccgggcgcgc cgccgccgcc gcggcgctcc ggcgcgaagc
     4501 cgcgcgcctc gagcgtccgc cgcaggacta ggagcccgtc cgagtattgc ctgcggcggc
     4561 gggggggggg ggg
//
LOCUS       DASPGN010000203         6216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1440192, whole genome shotgun sequence.
ACCESSION   DASPGN010000203 DASPGN010000000
VERSION     DASPGN010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6216
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440192"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VD788_05745"
     CDS             complement(<1..507)
                     /locus_tag="VD788_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV75805.1"
                     /translation="MASDPVAVGSPPLLPLAEMPERTISNDGEAAELLAARRGDPHAA
                     RAIVARYGDSMIRTAWRVLGTYGARDADDVVQEAFIAALTTTALPRENLGAWLRAIVA
                     RKALDELRRKGRRGEQPLDGATTRLAAEDRLGSRVEVLAVRRALARLSPADRALLTLV
                     DLEGWSLAE"
     gene            complement(518..1015)
                     /locus_tag="VD788_05750"
     CDS             complement(518..1015)
                     /locus_tag="VD788_05750"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HXV75806.1"
                     /translation="MTKVQTGIRAIVTLSALGLAAASLSAAEPPRRGGGFRGPGLPLV
                     EVFRQLELSDEQRDRVRSTVDEWMWGELGELLRTQRSERRELERLILDPSADEAAILA
                     ASAAVNRTRERVAVERHRFGVALFEILDETQRAEALALIAELPERGRRGYGGGPPRRG
                     PAAGD"
     gene            complement(1110..4403)
                     /locus_tag="VD788_05755"
     CDS             complement(1110..4403)
                     /locus_tag="VD788_05755"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75807.1"
                     /translation="MRSRAGRMAVWGLLVLAASAPAAGKGRNDTAEDESGPMSAARFK
                     GLEWRGIGPALMSGRIADIALHPTDAGTWYVAAGSGHLWKTTNAGTTWQPLFEDQPAY
                     SLGCVTIDPAHPEVIWLGTGENVGGRHVGYGDGVYRSLDAGKTWTRMGLERSEHIGRI
                     VVDPRDSNVVWVAAQGPLWAPGGDRGLFKTTDGGESWHNVLSAGEYTGVNDVVIDPRD
                     PDVLYAATHQRFRNVAALIDGGPESGIHKTTDGGKTWHELTQGLPEEEMGRIGLAISP
                     QRPDVLYAAIELAGRKGGVWRSDNAGGSWEKRSDYVAGGTGPHYYQEIFASPHRFDRL
                     YHMDVRLQVSEDGGKTWTNIEGEFKHVDNHAMAFDDDPDYLIVGCDGGVYESWDLGQT
                     WKFVANLPLTQFYKVAVDYDEPFYNLYGGTQDNNTQGGPSRTDNVHGIRNSDWFITLG
                     GDGHQPAVDPSNPDIVYSEWQGGNLTRFDRKTGEVVYIQPQPAEDEPSERFNWDAPIL
                     ISPHDPARLFYASHRVWQSDDRGDSWRPISGDLTRDLDRLELPMMGRVHSFDAVWDVY
                     AMSQYGTITSLAQSPLEAKLIYAGTDDGLIQVSENGGDSWRRIDKLPGAPERFFVNDI
                     KADLFDVDTVYVAVDDHKSGDFSPYLLKSTDRGKSWRSIVGDLPERHILWRLVQDHVE
                     RELLFVGTEFGVFFTIDGGKQWIKLEGGVPNIPFRDLAIQQRENDLVGATFGRGFYIL
                     DDYTPLRQVTPELLEERTSLFPVRKAWWYVPRRELGTDARASQGDAFFVAPNPPFGAV
                     FTYYLSETIRTRKAARREREKPIEQEAGDTPYPGWDELRREETEEDPAIVLTVRDARG
                     DVVRRLAGPVDRGFHRVAWDLRYPSTTPWSEASTGDDEDDEPQGALAPPGSYSVSLAE
                     RVDGVTTDLGQTRTFEVVRMRGGTLPGSGPERLVAFLERLAETQRQVQGARRVVDDTT
                     LALRAIREALMRSTADGGELGSAARALEQRLFEIRERLAGNERRQVYGDPGPVSIARR
                     LQVASVGTRVSTYGPTPTHIESLAIAERQFAELRRDLDRLVDVELPELERRLDEAGVP
                     WTPGRRVPG"
     gene            complement(4433..5449)
                     /locus_tag="VD788_05760"
     CDS             complement(4433..5449)
                     /locus_tag="VD788_05760"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HXV75808.1"
                     /translation="MRYLVTVDPGLEQVAADELRQLDPSAAVRVDGRARLVVEATDGA
                     WLFRARTIHHVARLAVEARAGTLDELRAAIAGTALPELERAASFRVSANTTGPVSFPR
                     RELAGAAGAVLQRRYGTRVDLERFEVNVRIDLDGERLLCGVQLTRESLGGRIHRGEPL
                     RTSLKPTVAAAMLRLAGAHSGSGRLIDPMCGAATIPIEAASINPRLSISAADWDGPTV
                     ALARRALAGQGIALEIDRADARLLGRDRPRAFDYIVTDPPYGVRLAKRTSLTRLYGAL
                     LPSFERALAERGRIVMIVVKYRTFLAALATTALEIVDRHAVELGGLEPQIVVLRHPGP
                     AVAD"
     gene            5593..>6216
                     /locus_tag="VD788_05765"
     CDS             5593..>6216
                     /locus_tag="VD788_05765"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF009668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXV75809.1"
                     /translation="MPIRLGDNAPNFKAQTTEGEIDFYEWMGDNWAVLFSHPKDFTPV
                     CTTELGMVAKLNPEFKKRGIKVIGLSVDPLGDHRRWSNDIAETQGVELNFPLIADADR
                     SVSDLYDMIHPNADDTLTVRSVFVIDNAKKVRLTLTYPASTGRNFDELLRSIDSLKLT
                     SEHKVATPVNWRHGEDVIIVPAVTNDEAKKKYPKGWREEKPYLRYVPD"
BASE COUNT          888 a         2263 c         2126 g          939 t
ORIGIN      
        1 ctcggccagc gaccagccct cgagatcgac gagcgtcagc agcgcacggt cggccggcga
       61 cagccgggcg agcgcccgcc gcaccgccag cacctcgacc cggctgccga gacggtcctc
      121 ggccgcgagt cgcgtcgtcg cgccgtcgag cggctgctcg ccccgccggc ccttgcgccg
      181 caactcgtcg agcgccttgc gcgcgacgat cgcgcgcagc cacgcgccga ggttctcccg
      241 cggcaacgcc gtggtggtca acgcggcgat gaacgcctcc tgcacgacgt cgtcggcgtc
      301 gcgcgcgccg tacgtcccga gcacgcgcca ggcggttcgg atcatcgaat cgccgtagcg
      361 ggcgacgatc gcccgggccg cgtgcggatc gcctcgcctg gcggccaaga gctcggcggc
      421 ttcgccgtcg ttcgagatcg tgcgctccgg catctccgcc aacggtaaca gcggcggcga
      481 cccgacggcc accggatcgc tggccacgcg tacccactca gtcgccggcg gccggtccgc
      541 gccgcggcgg cccgccgccg tatccgcggc gccctcgctc gggcagctct gcaatcagcg
      601 ccagcgcctc ggcacgctgc gtctcgtcca ggatctcgaa cagcgcgacg ccgaagcgat
      661 gacgctcgac cgccacgcgt tcccgcgtcc ggttgaccgc ggcggacgcg gccaggatcg
      721 cggcctcgtc ggccgacggg tcgaggatca gccgctcgag ctccctgcgc tcgctgcgct
      781 gggtccgcag cagctcgccc aactcgcccc acatccactc gtcgaccgtc gagcggacgc
      841 ggtcccgctg ctcgtccgac agctcgagct gccggaatac ctcgaccagc ggcagccccg
      901 ggccgcggaa gccaccgccg cggcggggcg gctcggcggc cgacagcgac gcggccgcca
      961 ggccgagggc ggaaagcgtc acgatcgccc ggattcccgt ctgcaccttc gtcatttcga
     1021 actccttttc cgtacgcccc agggccgtcg ttcaccctgg agacgtacgg ggtcgcccgc
     1081 gggtatcacc gcccccgttg cggggcgcgt cagcccggga cgcggcgacc cggcgtccag
     1141 ggcacgccgg cctcgtcgag ccggcgctcg agctccggga gctcgacgtc gaccagccgg
     1201 tcgaggtcgc ggcgcagctc ggcgaactgg cgctcggcga tggcgaggct ctcgatgtgg
     1261 gtcggcgtcg gcccgtaggt cgacacgcgc gtgccgacgg aggcgacctg caggcggcgc
     1321 gcgatcgata ccggtccggg atcgccgtag acctgtcgcc gctcgttgcc ggcgagccgc
     1381 tcgcggatct cgaacaagcg ttgttcgagg gcgcgcgccg cactgccgag ctcgccgccg
     1441 tcggccgtcg atcgcatcag cgcttcgcgg atcgcgcgca acgcgagcgt cgtgtcgtcg
     1501 accacacggc gggcgccctg cacctgccgc tgcgtctcgg ccaaccgctc gaggaaggcg
     1561 acgagacgct cgggaccgct gcccgggagc gtgccgccgc gcatccgtac cacctcgaac
     1621 gtccgggtct gtccgagatc ggtcgtcacc ccgtcgacac gctcggccag gctgacgctg
     1681 tagcttcccg gcggggcgag ggcgccttgc ggttcgtcgt cctcgtcgtc gcctgtgctc
     1741 gcctcggacc acggcgtcgt cgacgggtag cgcagatccc aggccacgcg atggaagccc
     1801 cggtcgaccg ggccggccag ccggcgcacg acgtcgccgc gcgcgtcgcg cacggtcagc
     1861 acgatcgccg ggtcttcctc ggtctcctcg cgccgcagct cgtcccagcc ggggtacggc
     1921 gtgtcgcccg cctcctgctc gatcggcttc tcccgttcgc ggcgcgccgc tttgcgcgtg
     1981 cggatcgtct cgctcagata gtaggtgaag accgcaccga acggcgggtt gggcgcgacg
     2041 aagaacgcat cgccctggct cgcgcgcgcg tccgtcccca gctcgcgccg cggcacgtac
     2101 caccacgcct tgcgcaccgg gaacagactc gtccgctcct cgagcagctc gggcgtcacc
     2161 tgccgcagcg gtgtgtagtc gtcgaggatg tagaagccgc ggccgaacgt cgcgccgacg
     2221 aggtcgttct cccgctgctg gatcgccaga tcgcggaacg ggatgttcgg cacaccccct
     2281 tcgagcttga tccactgctt gccgccgtcg atggtgaaga agacgccgaa ctcggtgccg
     2341 acgaacagca gctcgcgctc gacgtggtcc tgcacgaggc gccagaggat gtgtcgctcg
     2401 ggaaggtcgc cgacaatgct gcgccagctc ttgccgcggt cggtgctctt caacaggtac
     2461 ggtgagaaat cgcccgactt gtggtcgtcg acggcgacgt agaccgtgtc gacatcgaac
     2521 aggtcggcct tgatgtcgtt gacgaagaac cgctcgggcg cgccgggcag cttgtcgatc
     2581 cggcgccagc tgtcgccgcc gttctcgctg acctggatca gcccgtcgtc ggtgccggcg
     2641 tagatcagct tcgcctcgag cggcgactgg gccagcgagg tgatcgtgcc gtactgcgac
     2701 atcgcgtaga cgtcccacac cgcgtcgaag ctgtggacgc gacccatcat cggcagctcg
     2761 agccggtcga ggtcgcgcgt caggtcgccg ctgatcggcc gccagctgtc accgcgatcg
     2821 tcgctttgcc agacgcgatg gctcgcgtag aacagccggg cggggtcgtg cggactgatc
     2881 aggatcggcg cgtcccagtt gaagcgctcc gacggctcgt cctcggccgg ctggggctgg
     2941 atgtagacca cctcgcccgt cttgcggtcg aaacgcgtca ggttgccgcc ctgccattcg
     3001 gaatagacga tgtcggggtt cgacggatcg accgccggct ggtggccgtc gccccccagc
     3061 gtgatgaacc agtcgctgtt gcggatgccg tgcacgttgt cggtgcggga cgggccgccc
     3121 tgcgtgttgt tgtcctgcgt accgccgtag aggttgtaga acggctcgtc gtagtcgacc
     3181 gcgaccttgt agaactgggt cagcggcagg ttggcgacga acttccacgt ctggcccagg
     3241 tcccagctct cgtagacgcc gccgtcgcag ccgacgatca ggtagtccgg atcgtcgtcg
     3301 aacgccatcg cgtggttgtc gacgtgcttg aactcgccct cgatgttggt ccaggtcttg
     3361 ccgccgtcct ccgagacctg caggcggacg tccatgtggt acaggcgatc gaagcggtgc
     3421 ggactggcga agatctcctg atagtagtgc gggccggtgc cgccggcgac gtagtcgctc
     3481 cgcttctccc agctgccccc ggcgttgtcg ctgcgccaga cgccgccctt gcgcccggcc
     3541 agctcgatcg cggcgtagag cacgtcgggg cgctgcggcg agatggcgag gccgatgcga
     3601 cccatctcct cctcgggcag cccctgggtc agctcgtgcc acgtcttgcc gccgtcggtc
     3661 gtcttgtgga tgcccgattc gggcccgccg tcgatcagcg cggcgacgtt gcggaagcgc
     3721 tggtgcgtgg cggcgtagag cacgtcggga tcgcgcgggt cgatcacgac gtcgttgacc
     3781 ccggtgtact cgccggcgga cagcacgttg tgccagctct cgccgccgtc ggtcgtcttg
     3841 aacagaccgc ggtcgccgcc cggcgcccac aacggaccct gcgccgcgac ccagaccacg
     3901 ttcgagtcgc gcgggtcgac gacgatcctg ccgatgtgct cggagcgctc gagccccatc
     3961 cgggtccacg tcttgccggc gtcgaggctg cgatagacgc cgtcgccgta gcccacgtgc
     4021 cggccgccga cgttctcgcc ggtgccgagc cagatcacct cggggtgggc cgggtcgatc
     4081 gtcacgcaac cgagcgagta cgccggttgg tcctcgaaca acggttgcca ggtcgtgccg
     4141 gcgttcgtcg tcttccacag gtggccgctg ccggccgcca cgtaccacgt gccggcgtcg
     4201 gtcgggtgca gcgcgatgtc ggcgatccgc cccgacatca gcgcgggtcc gatgccacgc
     4261 cactcgagcc ccttgaacct ggcggcgctc atcgggcccg actcgtcctc ggccgtgtcg
     4321 ttccgcccct tgcccgccgc cggcgccgac gccgcgagca cgagcaaccc ccacaccgcc
     4381 atccgaccgg ctctcgatct catcgctccc ccccttaccc gttgatcgcg gctcagtctg
     4441 ccacagctgg tcctgggtga cgcagcacga cgatctgcgg ctcgagcccg cccagctcga
     4501 ccgcgtgccg atcgacgatc tcgagcgcgg tcgtcgcgag cgcggcgagg aaggtgcggt
     4561 acttcaccac gatcatcacg atgcgcccgc gctcggccag cgcgcgctcg aacgacggca
     4621 gcagcgcacc gtacagacgg gtcaggctgg tgcgcttggc cagccgcacg ccgtacggcg
     4681 ggtcggtcac gatgtagtcg aacgcgcggg gacggtcgcg ccccagcagg cgtgcgtcgg
     4741 cccggtcgat ctcgagcgcg ataccctgcc cggcgagcgc gcgccgcgcc aaggcgaccg
     4801 tcggtccgtc ccagtcggcg gccgagatcg acagccgcgg gttgatcgac gcggcctcga
     4861 tcgggatcgt cgccgccccg cacatcgggt cgatcaaccg cccgctgccg gagtgcgcgc
     4921 cggccagacg cagcatcgcc gccgcgacgg tcggcttgag cgacgtgcgc aacggctcgc
     4981 cgcggtggat gcgcccgccg agcgactcgc gcgtcagctg cacgccgcac agcagtcgct
     5041 cgccgtcgag gtcgatccgc acgttgacct cgaaccgctc gagatcgacg cgcgtcccgt
     5101 agcgtcgctg cagcacggca ccggccgcgc ccgccagctc gcgccgcggg aacgagacgg
     5161 gtccggtcgt gttggcgctg acgcggaacg acgccgcccg ctcgagctcg ggcagcgcgg
     5221 tcccggcgat cgcggcgcgc agctcgtcga gcgtcccggc gcgtgcttcg accgcgagcc
     5281 gtgcgacgtg gtggatcgtc ctcgcccgga acagccacgc gccgtccgtc gcttcgacga
     5341 cgagccgcgc gcgaccgtcg acgcggaccg cagccgacgg gtcgagctgg cggagctcgt
     5401 cggccgccac ctgctcgagg cccggatcga cggtcaccag gtatcgcatc ggctgtcgcc
     5461 ctcgcgcgcg ggaggcgagt ttctcacggc ggcggccggc ggagcggccg cggtcgtgcc
     5521 aagagccgcg attcgtgcca tagtggatca gtagcttcgc gccgaaaaaa agaaaatcga
     5581 aggagagatg ccatgccgat tcgcttgggg gacaacgctc cgaacttcaa ggcccagacg
     5641 accgaaggcg agatcgattt ttacgagtgg atgggggaca actgggccgt cctgttctcg
     5701 cacccgaaag acttcacacc ggtctgcacc accgagctcg gcatggtggc caagctcaac
     5761 cccgagttca agaagcgcgg gatcaaggtc atcgggctga gcgtcgatcc gttgggcgat
     5821 catcgacgct ggtcgaacga catcgcggag acgcagggcg tcgagctgaa cttcccgctg
     5881 atcgccgacg ccgaccgctc ggtttccgac ctgtacgaca tgatccaccc caacgccgac
     5941 gacacgctga ccgtgcgctc ggtcttcgtc atcgacaacg ccaagaaggt ccggttgacg
     6001 ctgacctacc cggccagcac cgggcgtaat ttcgacgagc tgctgcggtc gatcgactcg
     6061 ctcaagctga cgagcgagca caaggtcgcc acgccggtca actggcggca cggcgaggac
     6121 gtgatcatcg tcccggcggt gaccaacgac gaggccaaga agaagtaccc caagggctgg
     6181 cgcgaggaga agccctactt gcgctacgtc cccgac
//
LOCUS       DASPGN010000204        18412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_737918, whole genome shotgun sequence.
ACCESSION   DASPGN010000204 DASPGN010000000
VERSION     DASPGN010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 18412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18412
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737918"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1372
                     /locus_tag="VD788_05770"
     CDS             89..1372
                     /locus_tag="VD788_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXV75810.1"
                     /translation="MAGEILFVGDIHLGRRPRRLPDDLAEHGISPGELTPAAAFANVV
                     RYAVDAGVAAVVLAGDVVQSDNARFEAYGQLQRGVTTLTRHGIAVYAVAGNHDVEALP
                     RLADAIDGFELVGRGGRWEERIVRAGGRPVARLLGWSFARAEQTSSPLAQSDMPRFAA
                     ADGLPRLGVLHCDLLSSASRHAPVTVGELERAPVDGWLLGHVHQPSALGGARPIGYLG
                     SLVGLDPSETGRHGPWLLRIGDDATIAVEPLSLGPLRWESLDVPVDGLDDADAIECAI
                     REAMTAAAAGDQTRVLGCRIRLTGATRRIDAARRIESGGIERLRLQHGSVLCFVERLI
                     NAARPEIDLQSLAVQDDPPGILATKLLALERGDDEARSLIQRARRELQRSLDDRRWLR
                     LPATPPDDDALRALLIDAGRLALGELLGQSAERRR"
     gene            1369..4830
                     /locus_tag="VD788_05775"
     CDS             1369..4830
                     /locus_tag="VD788_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV75811.1"
                     /translation="MKLRRLTVHRMPGIDRGFTIDGLADGVNLIVGPNGSGKTTLCRA
                     LRAAVWPAIDRGRGITVEASWRTDRERWSVRRDGERVVWQRDGSPCEPPVTTDAHAAP
                     CFTLDFCDLLLDRADTDETIAAEIRRQISGGYDIDVIVARTLGLTERHGLHEERRLRE
                     RSRDLELVRRSHSELERREARLIELEQEIAEAAEAAARIDSLRAALELGRTRRRIAEL
                     EAGLAGLPPGTGVVRGDEARRLARIDEERRELERSHADASATVGRLRASRDALGLRSG
                     PVAEIELATALDRARRLQETESELRLARRQLTEAEAARASAADGLDRARVGGQAPRID
                     DASLAELEAFVDEAAKLRDRRQRLVEERDRTAVAAPSAPAAELDRAVSALRDWLAASS
                     LGLGFTVGLAVCAVVAVGAGLAVWHAGGQVAPGQGMVTGGVVLLAAAVVAALAEPARR
                     RRRVARRRFSGTALAPPSAWSHDPVELRLREVERLRSERATADERLRRAGELEAMLSA
                     MEREQHALDERRAELRRRLGVDPGGGELAPLRLAERVAAFQRADDDERRARAVVARLE
                     REREESAETLVAFLARAGTRPSPPDGPSLSAALADLGDRGRRFRHLEEQSAQQSRRIE
                     ELERQLDVVRARRAAVFGELGLEPGDDDGLRSRLDARPSWIDLSAAAGAERARLSDLE
                     RRAAHDPVATTMDEAAVVRALERAERVAARRDGLVDERGAIRSEVARAQAGQLLEEAT
                     AAVDEASEALAARRDEALFNTAGRFLLAEVGKEFDRRSRPELLARAGDLFARFTHHRY
                     QLEVDTSASPPAFRAVESETDQGKSLVELSDATRVQLLLAARLAFVSHVDRQARLPLL
                     LDEVLATSDPERFVAIAESLLLLAREQGRQVLYLTSDPADVARWQSVLSAAGEPPVET
                     IDLAALREMSSGIADERELSVPPLPRVPAPGNADAEQYGARLRVPRFDPTRPANSVHL
                     FHLLRDELPLLHRLLEIGVRTVGQWRRLAATDDGRRMLGEGAATRLSALADLVEAYVD
                     AWRIGRGRPVDAAALEEAELSPTWIERLGPLVERLGGDARRLIEALADRDDECTKGFQ
                     ARLRDRLRERLARSGHLDEREILDEHALRARVHAAVAFHTERGTLEPDEVAARMHEWI
                     HHARVHA"
     gene            4817..6235
                     /locus_tag="VD788_05780"
     CDS             4817..6235
                     /locus_tag="VD788_05780"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HXV75812.1"
                     /translation="MSTLEALLLAVGAMATPAVAAEFDEWFTGATIRVDYAHVGTAAE
                     EHFALERVRVEGPWPGSRTRTIDTTNLGRYLVELVDLESNRVLYTRGFASIFGEWEST
                     SAARSGVWRSIAEAVRVPEPKRPFQLRIRKRDAGLGFAEVWQTTIDPSSRFVDRVPPA
                     DHAHRVLLDHGDPAIKVDLVVLGDGYTAGEIERFRDDAAAAAAALFAEEPFAARTSSF
                     NVRIVETPARASGITRPRAGVFRDSPLGARYDTFDSERYVLTLDDRAWRDRAAVVPYD
                     FVLILVNERQYGGGGIHGRYSTAAAGSSYADYLVVHEFGHHFAGLGDEYYTSDVAYES
                     TDGPLVEPWEPNVTAQFDPARLKWGELVTEGTALPTPWVKQAYEQQSLDLQQRRRELR
                     ARGAPEEELERLFDEERERLTDLLRADPNAGIVGAFEGAMYRPNGLYRPAVDCIMFTR
                     DRVGFCPVCRRAIERVIEMYDR"
     gene            complement(6159..6548)
                     /locus_tag="VD788_05785"
     CDS             complement(6159..6548)
                     /locus_tag="VD788_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXV75813.1"
                     /translation="MGRERWYVYLIRTRGGNLYTGVATDVARRVAEHERSGARAARAL
                     RGKGPLRLVFQREIGERRLAQRVESRIKRLDRAGKEQLLHADPDVAQLLALLSLVADE
                     PIVSGRTSRSLVRSRDGRPDRIRPGRG"
     gene            complement(6570..11228)
                     /locus_tag="VD788_05790"
     CDS             complement(6570..11228)
                     /locus_tag="VD788_05790"
                     /inference="COORDINATES: protein motif:HMM:NF023149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malectin domain-containing carbohydrate-binding
                     protein"
                     /protein_id="HXV75814.1"
                     /translation="MSGPSGAWRFPIVFAAIAGSALALGAAEPWPRRVERGPVAAPAG
                     LAAEAFVAVTANGAIDASTFGAGSFVVTNAAATGGPRISSLTLDLSAAIFPDLVFDPL
                     GDAGDATAKCLTADSGAATTGYVAPSDPCADPHSAPHDGGYDVLTLSFTDFDPGESFS
                     FSVDVDPTSIQTAGGAGGAGSVSGLELTGSVLTIGFDTQESFATTLFRTPASQGGSQN
                     RVKAGGPPAPSVEILGVGTTPAIVTSATQTARVSAPAGSEVRLLVIEGALDLGQAPGF
                     DIDPFEANTAVAVQELTGTIPAGGSLDFALTLTAAGPAGGLNHVVAAILDGDGSGRTG
                     PSSAVRLLDLQPAPVINFLTGDLGGVSVNNPTSLDFGPDGRLYVAQQNGTLQAFTISR
                     VGLGDYQATSTETIGLIRQIPNHDDDGSVNAAQTNRQVTGLLAAGSALAPVIYVTSSD
                     PRIGAGGGGHDSNLDTNSGVISRLTWTGSAWESVHLVRGLPRSEENHSTNGMALDETS
                     GTLYVMSGGHTNMGAASNNFAQTQEYALAAALLAVDLTAIDAMPTQVDGEGQSYKYDL
                     PTLDDPLRGGEPDPGDPFGGNDGRNQAIVVPGGPVAVYAPGFRNAYDVVLTESGRLYT
                     VDNGPNAGWGGVPIGEGGASCTNQTNETGSNTYGDGLHFISGPGYYGGHPHPTRANPE
                     DSGLYIYENQGGSWVLTESYDWATDFPLPPVPLGTGNVVECDYRQPGVDDQALAVISA
                     STNGITEYRATNFAGTMQGDLLAASFNGNVYRFELNGAGDALAAPEQALFAGFGGQPL
                     DVTAQADGEPFPGTVWVAVYGADKITVFEPVDFTSCSGVDDPTFDEDGDGYDNADEID
                     AGSNPCSGGSRPTDNDGDLTSDRNDPDDDNDGINDPHDAFATDADNGTTTGLPVIYPF
                     FNADPGTGFFGLGFTGLMANGATDWLDQFDETQLAAGGAAGLFTVEQVGAGDALNGAN
                     DQQNGFLFGIDVDSASSVFSVQTRLLSPYFQVGGAPSTPIDFQSFGLFVGNGDQDDYL
                     KLVLNAQGGAGGVQLLYEQGGISSATDYDTSVVGDVLSSIFLDLYLTIDPAMLTVQPH
                     LAVDGAPLIDLGTPISIPAEWLDPADANGLAVGLISTSAGSGVPFGGTWDFIEVTAGA
                     AGTECLSDLDCDDGEVCTVDRCDDGLCENGAGNPGLTCEADDDLCTIDECNGLGSCLT
                     AGTIACEPDEMCNPATGACEPSVLDADNDGLYNALDPCPADARNRCFGSVAQDAATGN
                     AIRINAGTGQACSGSRIDCNGDTWLGDFGFNTGNSFACNLANGCPIGGLDPIFGGGCA
                     GHAATEDLFRCERWDDAVEPELQYGFDVPPGGYVVNLLFANAWDGSSQPTERVFDIVI
                     EEQLVHDDFDQVLAAAGSGMAVVRSAVVDVAGPGGLQIEFRHVVQNPAIKAIEVLACS
                     ATAPVASTTLALAKQPGGAALSWQPIAAAFSHDVVRGDLALLRGSAGDFASSVSDCAG
                     DNLSGSSLLDPDPLPAGGGFWYLVRGSNCGGNSSWDSGGAGQHAPRDAGIDAGALSCP
                     "
     gene            complement(11332..11988)
                     /locus_tag="VD788_05795"
     CDS             complement(11332..11988)
                     /locus_tag="VD788_05795"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HXV75815.1"
                     /translation="MPTSTVEDYLKCIYLESRGAPDERASTGRIATALNVAPGTVTAM
                     MKTLTESGLVGYEPYAGVRLTEAGLRLATHVLRRHRLVELFLVRIMGMDWSEVHEEAE
                     VLEHAVSERLIERIDSMLGQPSVDPHGDPIPTAGGEIDEPELDDLLTCAVRRRLTVAR
                     VTDQRTEFLQLLDRHGVTPGQSVLVLSRDTLTETVRLDTRDGGGLQLGFRAASKILVR
                     "
     gene            complement(12015..13406)
                     /locus_tag="VD788_05800"
     CDS             complement(12015..13406)
                     /locus_tag="VD788_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXV75816.1"
                     /translation="MGCHRLVCAVALMLFAVGPAAPRPAAAAARQGGEIGLQAGLLLP
                     DRDLTGEPTTLERLEPTLGIRGAFQFAPRWGWFVDSNLAVFESAAGDGITNYAARSGV
                     ELFGAPHWTDAQTFVALGGGWLGVARESRDDFNRLFGSIGVGQRFTVGPNQLLRWELR
                     VDQTLTDDGADGAALTTGLLLVGFGWGMPSRAADSDGDGVPDRKDACPDTPRGARVDT
                     QGCPQDGDGDGVADGIDQCPDTPAGWAVNGAGCPLDSDRDGVTDGEDDCPGTPPAVTV
                     DDRGCPQDGDGDGVADGIDRCPDTPEFAHVDADGCPLDSDADGVYDGIDRCPQTAAGA
                     RVDPDGCPVAAPLFEERASLVLDGVFFEVNSAELTLNSKEILDDVAASLLASPEVPVE
                     VGGFTDSSGNDGYNLALSHKRAEAVRAQLIAQGVAPERLTTRGYGEASPIADNTTTEG
                     RARNRRVELRRLD"
     gene            complement(13486..16197)
                     /locus_tag="VD788_05805"
     CDS             complement(13486..16197)
                     /locus_tag="VD788_05805"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HXV75817.1"
                     /translation="MRSELSAMLEPGRVLTRLIDRVAYANDASVYRLVPQAVVFPRSL
                     DEVRQLFAFSHRRGIPLTFRSAGTSLSGQAVSDGLLVVLSRHWDAIEVREHGRAVWVQ
                     PGVLGGTVNRHLEPYGTKIGPDPASINACMMGGILANNSSGMCCGVERNAYHTLQSIH
                     FLLPDGVEIDSAAPDADERLRRESPALADGLVELSRRVRRDSALAARIRAKYEMKNTN
                     GYSLNALLDHDSAVEILSHLMIGSEGTLGFIADAALRTVPDHALKYTGLLLFRGVEQA
                     CAAIDELRRSGAEALELMDRASLRSIEHEPGVPPAIRGLPPGAAALLVEYQCGSRAEL
                     DERREACRRVLERLSLLRPAELSTDPVPRAALWRVRKGLIPSVGAMRRRGTSFILEDI
                     VFPVPQLARGVADLRALFERFAYDDAIVFGHAKDGNLHFVLVQAFDDPRDVERYDAFM
                     HALAEVVVGRYDGALKAEHGTGRNMAPFVEAEWGTDAYRVMREIKRLVDPRGLLNPGV
                     LLNDDPRAHIRHLKTLATVEAEVDRCIECGFCERMCPSRTLTLTPRQRIVVRREMARL
                     RAEADRSAELAELERDFVYDALDTCAADGLCALACPVAIDTGALVKRLRAEAHSPLAQ
                     RLAALGALRLAWVERLVRAALSAVRLARRDLPRPSRGLPRTRAGGATAVYFPSCVTRV
                     VESSATRLVAVAARAGRPVRIPGDVAGTCCGMPFSSKGYRRAQALAVNRCVERMWRWS
                     GGGELPIVLDTSPCALTLKSCEPELDATNRERFAALEILDGVEFAERLLAAGLRPTRP
                     KPSVVLHPVCSLSKMGLVERWAAVVAPFAERVEIPIDSGCCGFAGDRGYRVPELTAAA
                     TAVEAREVNRARHDGYYSTSRTCEIGLTRATGRSYDSFWRLLDEATV"
     gene            16289..16594
                     /locus_tag="VD788_05810"
     CDS             16289..16594
                     /locus_tag="VD788_05810"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HXV75818.1"
                     /translation="MPTYVLMTRLAPDSLHDARGRRAVGKSWLEQVRKACPDVEWLAH
                     YAILGPYDFMDIYRAPDEETAHKVSLISRSAGAVTAESWGALPYDRYLLLLEQVRPD"
     gene            16706..17167
                     /locus_tag="VD788_05815"
     CDS             16706..17167
                     /locus_tag="VD788_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75819.1"
                     /translation="MLVRVAEESFDKGVAALGDGRQHEALAFFEAAIELECKYGTGKP
                     QARYLSHYGLCLGLAGRRKHEAVKFCREAVAMEGYNPDLLWNLGRALLIADLRREAYA
                     AFVKGLRLEPGHKGIIQDLKAMGLRKRPPLPFLDRSNPINVLLGRIRSNGS"
     gene            17226..18266
                     /locus_tag="VD788_05820"
     CDS             17226..18266
                     /locus_tag="VD788_05820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV75820.1"
                     /translation="MSAQRRLAVLPVDDARGASTRYRVLAYLPALRAAGFDPHVHDPL
                     RTRGTLAGAARIADLTRDVAAASRAEVVLVHRKMYPPPFAGRLRRAASRLVLDIDDAV
                     DLPPPGRELDRRTRERYRRNFDATAAAADLVVCGNAELERRVPHERTVRLPTPVDTAR
                     FRPDALGPPRPLTLGWVGHSDNLRYLERLLPVLRELAGRHPAFSLIVVADRPPAIDGV
                     PIEFRRWSLEQEISCFDGIAVGLMPLEDGEWARSKCAFKAIQYMALGIPPVVSPVGMN
                     REVVEHGISGFLAADAGQWIEAIETLFSRPELAASIGAAGRRVVERRYSLAANAPLLI
                     DALRGVGRTARC"
BASE COUNT         2616 a         6554 c         6575 g         2667 t
ORIGIN      
        1 cccccccccc cccccccccc gccgccgcag gcaatactcg gacaggctcc tagcccgtcg
       61 cgccggccga tgtgcgatac tcgaacgcat ggccggcgag atcctgtttg tcggggacat
      121 ccacctgggt cgccgaccgc ggcgtctgcc cgatgacctg gccgagcacg ggatctcgcc
      181 cggcgagctg acgccggccg ccgcctttgc caacgtggtg cgctacgccg tcgacgccgg
      241 cgtcgcggcc gtcgtgctcg cgggcgacgt cgtgcagtcg gacaacgccc ggttcgaggc
      301 ctacggccag cttcaacggg gcgtgacgac gctgacgcga cacgggatcg cggtctatgc
      361 cgtcgccggc aaccatgacg tcgaggcgtt gccccgcctc gccgacgcga tcgacggctt
      421 cgagctcgtc gggcgcggcg ggcgctggga agagcggatc gtccgtgccg gcgggcgccc
      481 cgtcgcacga ctgctcggct ggtcgttcgc ccgcgccgag cagacgagca gtccgctggc
      541 gcagtcggac atgccgcgtt tcgccgcggc ggacggcttg ccccgtctcg gcgtgttgca
      601 ctgtgacctg ctctcgtcgg cgagccggca cgcgccggtc accgtcggtg agctcgaacg
      661 ggcgccggtc gacggctggc tgctcggtca cgtgcaccag ccctcggcgc tgggcggggc
      721 gcggccgatc ggctacctcg gctcgctggt cgggctcgac ccgagcgaaa ccggccggca
      781 cggaccgtgg ctgttgcgca tcggcgacga cgcgacgatc gcggtcgagc cgttgtcgct
      841 cggcccgctg cgctgggagt cgctcgacgt cccggtcgac ggcttggacg acgccgacgc
      901 gatcgagtgt gcgatccgcg aggcgatgac ggcagccgcc gccggcgacc agacccgcgt
      961 actcggttgc cggattcgcc tgaccggggc gacgcgccgg atcgacgccg cgcgccggat
     1021 cgaatccggc ggcatcgagc ggctgcgcct acagcacggc tcggtgttgt gcttcgtcga
     1081 gcgcttgatc aacgcggcgc gacccgagat cgatctccaa tcgctcgcgg tgcaggacga
     1141 cccgccgggg atcctcgcca ccaagctgct cgctctcgag cgcggcgacg acgaggcgcg
     1201 ctcgttgatc cagcgcgcgc gacgagagct ccagcgctcg ctcgacgacc gccgctggct
     1261 gagactgccg gcgactccgc ccgacgacga tgcgctgcgt gcgttgttga tcgacgccgg
     1321 ccggctggcg ctcggcgaac tgctcgggca gtcggcggag cgtcgtcgat gaagctgcgt
     1381 cgcttgaccg tccatcgcat gccggggatc gaccgcggct tcaccatcga cgggctggcc
     1441 gacggggtga acctgatcgt cgggcccaac gggtcgggca agacgacgct gtgccgcgct
     1501 ttgcgtgccg cggtctggcc cgcgatcgac cgcgggcggg gaatcaccgt cgaggccagt
     1561 tggcgcaccg accgggagcg gtggagtgtg agacgcgacg gcgagcgcgt cgtctggcag
     1621 cgcgacgggt cgccgtgtga gcctcccgtc accaccgacg cgcacgcggc gccttgcttc
     1681 acgctcgatt tttgcgatct gctgctcgat cgcgccgaca ccgacgagac gatcgccgcc
     1741 gagatccgcc gacagatctc cggcggctac gacatcgacg tcatcgtcgc gcggacattg
     1801 ggcttgaccg agcgccacgg cctccacgaa gagagacggt tgcgcgagcg ctcgcgcgac
     1861 ctcgagttgg tgcgccgatc gcacagcgag ctcgagcggc gcgaagcacg gctgatcgag
     1921 ctcgagcaag agatcgcgga ggcagcggaa gcggcagcgc ggatcgactc cctgcgggcc
     1981 gcgctcgagc tcggccggac gcgcagacgg atcgccgagc tcgaggccgg tcttgccggg
     2041 ctcccgccgg ggaccggcgt cgtgcgcggc gacgaggcgc gtcgcctggc gcggatcgac
     2101 gaggagcggc gggagctcga aaggtcgcac gccgacgcat ccgcgaccgt gggccgtctg
     2161 cgcgcgtcac gcgacgcgct cggtctgcgc tcgggcccgg tcgccgagat cgagctcgcg
     2221 acagcgctcg accgcgcgcg ccggctgcaa gaaacggaga gcgagctgcg actggcgcgc
     2281 cggcagttga ccgaagccga ggcggcccgc gcgtcggcgg cggatgggct cgaccgcgcg
     2341 cgggtcggcg gccaggcgcc ccgcatcgac gacgcttcgc tcgccgagct cgaggcgttc
     2401 gtcgacgagg cggccaagct gcgtgatcgg cgacagcggc tggtcgagga gcgcgacagg
     2461 acggccgtcg ccgcgccgtc cgcgccggcc gccgagctcg accgcgcggt ctccgcgctg
     2521 cgggactggc tcgcggcgag ctcgctcggt ctcggcttca ccgtcggtct cgcggtgtgc
     2581 gcggtcgtcg ccgtcggggc cggcctcgcc gtgtggcacg ccggcggcca ggtggcgccg
     2641 gggcaaggca tggtgaccgg cggggtggtg ctgctggcgg ccgcggtcgt cgcggcactc
     2701 gccgagcccg cacgccggcg acgccgcgtc gcacgacggc gattctccgg cacggcgctc
     2761 gcgccgccga gcgcctggag tcacgatccg gtcgaactgc gactacggga agtcgagcgg
     2821 ctgcgcagcg agcgcgcgac cgccgacgaa cgccttcgcc gcgccggcga gctcgaagcg
     2881 atgctgtcgg cgatggagcg cgagcaacac gcgctcgacg agcgccgggc cgagctgcgg
     2941 cggcgtctcg gcgtcgatcc gggtggcggc gagctggcgc cgttgcgttt ggccgagcgc
     3001 gtggcggcat tccagcgggc cgacgacgac gagcggcgcg caagggccgt cgtcgcgcgg
     3061 ctcgagcgcg aacgcgagga atcggccgag acgctcgtcg cctttctcgc gcgggccggc
     3121 acgcgcccgt cgccgccgga cgggccgtcg ctgtcggcgg cgctcgccga cctcggcgat
     3181 cggggccggc gcttccggca cctcgaggaa cagtcggcgc agcagtcgcg ccggatcgaa
     3241 gagctcgagc ggcaactcga cgtcgtccgg gccaggcgcg ccgcggtctt cggcgaactg
     3301 gggctcgagc cgggcgacga cgacggtttg cgatcgcgtc tcgacgcgcg cccgagctgg
     3361 atcgacttgt cggcggccgc cggcgccgag cgtgcccggc tctccgacct cgagcggcgt
     3421 gcggcacacg acccggtcgc gacgacgatg gacgaggcgg cggtcgtacg cgccctggag
     3481 cgggccgagc gggtcgccgc gcgccgggac ggtctggtcg acgaacgggg cgcgatacgc
     3541 agcgaggtcg cgcgcgcgca ggccggccaa ttactcgagg aagccaccgc cgccgtcgac
     3601 gaggcgagcg aggcgctcgc cgcccggcgg gacgaggcgt tgttcaacac cgcggggcgc
     3661 ttcctgctcg ccgaggtcgg taaggaattc gatcgccgca gtcggccgga gctgctcgcg
     3721 cgggccggcg atctgttcgc gcgattcacc caccaccgct accagctcga ggtcgacacg
     3781 agcgcgagcc ccccggcctt ccgcgccgtc gagagcgaga ccgaccaggg caagtcgctc
     3841 gtcgagttgt ccgatgcgac acgggtccaa ctgctgctcg cggcgcgtct cgcgttcgtc
     3901 tcccacgtcg accgccaggc gcgcctgccg ttgctgctcg acgaggtgct ggccacctcc
     3961 gaccccgagc gcttcgtcgc gatcgccgaa tcgctgctgc tgctcgcgcg cgaacaggga
     4021 cgccaggtgc tctatctgac ctccgacccg gcggacgtcg cgcgctggca gtcggtgctg
     4081 agcgccgcgg gcgagccgcc ggtcgagacg atcgatctcg ccgccttgcg cgagatgtcg
     4141 agcggcatcg ccgacgaacg cgagctgagc gtaccgccgt tgccacgggt gcccgcgccc
     4201 ggcaacgcgg acgccgagca gtacggcgcg cgcttgcggg tcccgcgctt cgacccgacc
     4261 cgacccgcca actcggtcca cctgtttcat ctgctgcgcg acgagctgcc gctcttgcac
     4321 cgtctgctcg agatcggcgt gcgcaccgtc ggccagtggc gtcggctggc cgcgaccgac
     4381 gacggccggc ggatgttggg cgagggtgcg gcgacgcggc tttcggcgct ggccgacttg
     4441 gtcgaggcct acgtcgacgc gtggcgcatc ggccgcgggc ggccggtcga cgcggcggcg
     4501 ctcgaggagg ccgagctgag tccgacgtgg atcgaacgac tcggcccgct ggtcgagcga
     4561 ctgggcggcg acgcgcggcg attgatcgaa gcgctggccg atcgggacga cgagtgcacc
     4621 aaggggttcc aggcacgcct gcgcgaccgg ctccgcgagc ggctcgcgcg cagcggccac
     4681 ctcgacgagc gcgagatcct cgacgagcac gcgctgcggg cccgcgttca cgcggcggtt
     4741 gcgttccaca ccgaacgcgg cacgctcgaa cccgacgagg ttgcggcccg catgcacgag
     4801 tggatccacc atgcgcgtgt ccacgcttga ggcactgctg ctcgcggtcg gggcgatggc
     4861 gacgccggcc gtcgcggccg agttcgacga gtggttcacc ggcgccacga tccgcgtgga
     4921 ctacgctcac gtcggcaccg cggccgagga gcacttcgcg ctcgagcggg tccgcgtcga
     4981 gggtccgtgg cccggcagcc gcacgcgaac gatcgacacg accaacctcg gtcgctacct
     5041 ggtcgaactg gtcgatctgg aatcgaatcg tgtgctctac actcgcggct tcgccagcat
     5101 cttcggtgag tgggaatcga ccagcgcagc gcgcagcggt gtctggcgct cgatcgccga
     5161 ggcggtccgc gttcccgagc ccaagcgccc gttccaattg aggatccgca aacgcgacgc
     5221 gggcctcggc ttcgccgagg tgtggcagac cacgatcgat ccctcgtcgc gcttcgtcga
     5281 ccgcgtgcca ccggcggacc acgcgcatcg tgtgctgctc gaccacggcg atcccgcgat
     5341 caaagtcgat ctggtggtgc tgggagacgg ctatacggcc ggcgagatcg agcggttccg
     5401 cgacgacgcg gccgcggcgg ccgcagccct gttcgccgag gagccgttcg ccgctcgcac
     5461 gagctcgttc aacgtccgga tcgtcgagac gccggcgcgc gcgagtggca ttacgcggcc
     5521 gcgcgcgggt gtctttcgcg actcgccgct cggcgcgcgg tacgacacgt tcgactcgga
     5581 gcgctatgtg ctcacgctcg acgaccgtgc gtggcgcgac cgtgccgccg tcgttccgta
     5641 cgacttcgtg ctgatcctgg tcaacgagcg ccagtacggc ggcggcggga tccatggccg
     5701 gtactcgacg gccgccgccg gatcgtccta cgcggactat ctggtggtcc atgagttcgg
     5761 ccaccatttc gccgggttgg gcgacgagta ctacacctcc gacgtcgcct acgaatcgac
     5821 cgacggtcca ctcgtcgagc cgtgggagcc gaacgtcacc gcgcagttcg atccggcgcg
     5881 gttgaagtgg ggcgagctcg tgaccgaggg gaccgcgctg ccgacgccgt gggtcaagca
     5941 ggcctacgaa cagcagtcgc tcgacttgca gcagcgccgg cgcgagctgc gcgcgcgcgg
     6001 cgcgcccgag gaagagctcg agcggttgtt cgacgaagag cgcgagcggt tgaccgacct
     6061 gctccgggcc gaccccaacg ccggcatcgt cggcgcgttc gagggcgcga tgtaccggcc
     6121 gaacggtctc taccggccgg cggtcgactg catcatgttc acccgcgacc gggtcggatt
     6181 ctgtccggtc tgccgtcgcg cgatcgaacg agtgatcgag atgtacgacc gctgacgatc
     6241 ggctcgtcgg cgaccagcga caacagcgcg agcagttggg ccacgtcggg gtcggcgtgg
     6301 aggagttgct ccttgccggc gcggtcgagc cgcttgatgc gggattcgac gcgctgcgcc
     6361 agccggcgct cgccgatctc gcgctggaac accaggcgga gcggcccttt gccgcgcaac
     6421 gcccgcgcag ctcgcgcacc ggatcgctcg tgctcggcca cgcgtcgcgc gacgtcggtg
     6481 gcgacgccgg tgtacaggtt tcccccgcgc gtgcggatca ggtagacgta ccagcgctcg
     6541 cgccccatcg ccgctcgagc atacgccaat caggggcagc tgagcgcccc ggcgtcgatg
     6601 ccggcgtcgc gcggcgcgtg ctgcccggcg ccgcccgaat cccaactcga gttcccgccg
     6661 caattcgacc cgcgcaccag ataccagaac ccgccgcccg ccggcagcgg atcgggatcg
     6721 agcagcgagc tccccgacag gttgtcgccc gcacagtcgc tgaccgagga ggcgaagtca
     6781 ccggccgagc cgcgcagcaa tgccaggtcg ccgcggacca cgtcgtgcga gaacgcggcg
     6841 gcgatcggct gccagctcaa cgccgcgcca cccggctgct tcgccaacgc gagcgtggtc
     6901 gacgcgaccg gagcggtcgc cgagcaggcg agcacctcga tcgccttgat cgccgggttc
     6961 tgcacgacgt gtcgaaactc gatctgcaac ccaccggggc cggcgacgtc gacgacggcc
     7021 gagcgcacga cggccattcc gctgcccgcg gcggccagta cctgatcgaa atcgtcgtgg
     7081 accagttgtt cttcgatgac gatgtcgaac acccgttcgg tgggctggga cgacccgtcc
     7141 caggcgttgg cgaacagcag gttgacgacg tagccgcccg gtggcacgtc gaagccgtat
     7201 tgcagctccg gctcgacggc gtcgtcccag cgctcgcacc ggaacaggtc ctccgtcgcg
     7261 gcgtggccgg cgcagccgcc gccgaagatc gggtcgaggc cgccgatcgg gcaaccgttg
     7321 gccaggttgc acgcgaacga attgccggtg ttgaagccga agtcgccgag ccacgtatcg
     7381 ccgttgcagt cgattctgct gcccgagcag gcttgccccg tgccggcgtt gatccggatc
     7441 gcgttgccgg tagcggcgtc ttgggccacg ctgccgaaac accgattgcg cgcgtccgcc
     7501 gggcagggat cgagcgcgtt gtacaacccg tcgttgtcgg catcgagcac ggacggctcg
     7561 caggcgccgg tcgccgggtt gcacatctcg tcgggctcgc aggcgatcgt ccccgcggtc
     7621 aggcacgagc ccagaccgtt gcactcgtcg atcgtgcaca ggtcgtcgtc ggcctcgcag
     7681 gtcagcccgg ggttgcccgc gccgttctcg cacaggccgt cgtcgcagcg gtcgaccgta
     7741 cagacctcgc cgtcgtcgca gtcgaggtcg ctcaggcact cggtccccgc cgcccccgct
     7801 gtgacctcga tgaagtccca cgtcccgccg aacggtacgc ccgagccggc cgaggtcgaa
     7861 atcaggccga ccgccagtcc gttggcgtcc gcggggtcga gccattccgc cggaatcgat
     7921 atcggcgtgc cgagatcgat caacggcgcg ccgtcgaccg ccaggtgggg ctgcaccgtc
     7981 agcatcgccg ggtcgatcgt cagatagagg tcgaggaaga tcgacgacag cacgtcgccg
     8041 acgaccgacg tgtcgtagtc ggtcgcgctg ctgatgccgc cctgctcgta caacagctgg
     8101 accccgcccg cgccgccttg ggcgttgagc acgagcttga ggtagtcgtc ctggtcgcca
     8161 ttgccgacga acagaccgaa ggactggaag tcgatcggcg tcgacggcgc gccgccgacc
     8221 tggaagtagg gcgacagcag acgtgtctgg accgagaaga cggaggacgc actgtcgacg
     8281 tcgatgccga acagaaagcc gttctgctgg tcgttggcgc cgttcagcgc gtcgcccgcg
     8341 ccgacctgct cgacggtgaa caggccggcg gcaccgccgg cggccagctg cgtctcgtcg
     8401 aactggtcca accagtcggt cgcgccgttg gccatcaggc cggtgaaacc gagcccgaag
     8461 aaaccggtgc cgggatccgc gttgaagaag gggtagatca cgggcagccc ggtcgtcgtg
     8521 ccgttgtcgg cgtcggtcgc gaacgcgtcg tgtggatcgt tgatgccatc gttgtcgtcg
     8581 tccgggtcgt tgcggtcgga ggtcagatcg ccgtcgttgt ccgtcggccg gctgccgccc
     8641 gagcaggggt tgctgcccgc gtcgatctcg tcggcgttgt cgtatccgtc gccgtcctcg
     8701 tcgaaggtcg gatcgtcgac tccgctgcag ctggtgaagt cgaccggctc gaacacggtg
     8761 atcttgtcgg ctccgtagac ggcgacccag accgtgccag ggaacggctc accgtcggcc
     8821 tgcgcggtga cgtcgagcgg ttggccgccg aagccggcga acagggcctg ctccggcgcc
     8881 gcgagcgcgt cgcccgcgcc gttgagctcg aagcgataga cgttgccgtt gaacgaggcg
     8941 gcgagcagat cgccctgcat cgtcccggcg aaattggtcg cgcggtactc ggtgatgccg
     9001 ttggtcgagg ccgagatcac cgccagcgcc tggtcgtcga cgcccggctg ccggtagtcg
     9061 cattcgacga cgttgcccgt gccgagcggc accggcggca gtgggaagtc cgttgcccaa
     9121 tcgtagctct cggtcagcac ccacgacccc ccctggttct cgtagatgta cagccccgag
     9181 tcctcggggt tggcgcgcgt cgggtggggg tggccgccgt agtaacccgg cccggagatg
     9241 aagtgcaggc cgtcaccgta ggtgttgctg ccggtctcgt tcgtctggtt ggtgcaggac
     9301 gcgccgccct cgccgatcgg gactccgccc cacccggcgt tggggccgtt gtcgacggtg
     9361 tacagccgtc cggactcggt cagcaccacg tcgtacgcgt tgcggaaccc gggcgcgtag
     9421 accgccaccg gcccgcccgg aacgacgatc gcctggttgc gcccgtcgtt gcccccgaac
     9481 gggtcgcccg ggtcgggctc gcccccgcgc agcgggtcgt cgagcgtcgg caggtcgtac
     9541 ttgtagctct gcccctcgcc gtcgacctgg gtcggcatcg cgtcgatcgc ggtcagatcg
     9601 accgcgagca acgcggccgc cagcgcgtac tcctgtgttt gggcgaagtt gttgctggcc
     9661 gcccccatgt tggtatgacc gcccgacatc acgtagagcg tgccgctcgt ctcgtcgagc
     9721 gccatcccgt tggtcgagtg gttctcctcc gaacgcggca gcccgcgaac gagatgcacc
     9781 gactcccatg cgctcccggt ccaggtcaat cgggagatca cccccgagtt ggtgtcgaga
     9841 ttcgagtcgt gtccgccccc accggcgccg atccgcggat cggacgaggt gacgtagatc
     9901 acgggcgcca gcgcgctccc ggctgcgagc agcccggtga cctgacgatt ggtctgggcg
     9961 gcattgacgc tgccgtcgtc gtcgtggttc gggatctgcc ggatcagacc gatcgtctcg
    10021 gtcgacgtcg cctggtagtc gccgagcccg acgcgagaga tggtgaacgc ctgcagcgtg
    10081 ccgttctgct gcgccacgta cagccggccg tcggggccga agtcgagcga ggtcgggttg
    10141 ttgacgctga ctccgccgag gtcgcccgtc aggaagttga tcaccggggc cggctgcagg
    10201 tcgagcagcc gaaccgccga gctcggtccg gtgcggccgc tgccatcgcc gtcgaggatc
    10261 gcggccacga cgtggttcaa gccgcccgcc ggcccggcgg cggtcagcgt cagtgcgaag
    10321 tcgagcgagc cgccagccgg gatcgtgccg gtgagctcct ggaccgccac ggcggtgttg
    10381 gcttcgaacg ggtcgatgtc gaagcccggc gcctgaccca ggtcgagtgc gccctcgatc
    10441 accagcagac ggacctcgga gccggcgggc gcgctgaccc gggcggtctg cgtggccgag
    10501 gtgacgatcg ccggggtcgt gccgacgccg aggatctcga ccgacggggc gggcggcccg
    10561 ccggccttga cgcggttctg cgagccgccc tggctcgccg gcgttcggaa caaggtcgtg
    10621 gcgaacgact cctgcgtgtc gaacccgatc gtcagcaccg atccggtcag ctcgagaccg
    10681 gagaccgacc ccgcgccgcc ggcgccgccc gcggtctgga tgctcgtcgg atcgacgtcg
    10741 accgagaacg agaagctctc ccccgggtcg aaatcggtga acgacagcgt cagcacgtcg
    10801 taaccgccgt cgtgcggcgc ggagtgtggg tcggcgcagg gatcggacgg cgcgacgtac
    10861 cccgtcgtcg ccgccccgct gtccgcggtc agacacttgg cggtcgcgtc gccggcgtcg
    10921 ccgagcgggt cgaagacgag gtcgggaaag atcgcggcgg acagatcgag ggtcagcgag
    10981 ctgatacgcg gtccgccggt cgcggcggcg ttcgtcacga cgaacgagcc ggccccgaag
    11041 gtgctggcgt cgatcgcgcc gttcgcggtg acggcgacga acgcctccgc tgcgaggccc
    11101 gccggagcgg ccaccgggcc gcgctcgacg cgccggggcc acggctcggc ggcgcccagc
    11161 gcgagcgccg agccggcgat tgcggcgaac acgatcggga aacgccaggc tccgctcgga
    11221 ccgctcatct cgacaccccg cgccacgcgc accacgccgg ccccgttcgc cgacggtgcc
    11281 tatcagttac gacctctcgg tcggccgttc aaccgtcggc cgcggacggg gtcaacgcac
    11341 caggattttc gaggcggccc ggaagccgag ctgcagcccg cccccgtcgc gggtgtcgag
    11401 tcgcacggtc tcggtcagcg tgtcacggga caggacgagc accgattggc ccggcgtcac
    11461 gccgtgccgg tcgagcagct gcaggaactc ggtccgctgg tcggtgacgc gtgccacggt
    11521 cagccggcgc cggaccgcgc aggtcagcag gtcgtcgagc tccggttcgt cgatctcgcc
    11581 gccggccgtc gggatcgggt ccccgtgcgg atcgaccgag ggctggccca gcatcgagtc
    11641 gatccgctcg atcagccgct cggaaaccgc gtgctcgagc acctccgcct cctcgtggac
    11701 ctcgctccag tccatcccca tgatacggac caggaacagc tcgaccagtc ggtgccgcct
    11761 cagcacgtgc gtcgccagcc gcagcccggc ttcggtcagc cgcacgccgg cgtagggttc
    11821 gtagccgacg agaccggact cggtcagcgt cttcatcatc gcggtgaccg ttccgggggc
    11881 gacgttcaac gcggtggcga tccgccccgt cgacgctcgt tcgtccggcg cgccgcgcga
    11941 ctcgaggtag atgcacttga ggtagtcctc gacggtgctg gtcggcatgc cgagagcata
    12001 acccaaccgc ggcgtcagtc gaggcgacgc aactcgacgc ggcggttgcg cgcgcggccc
    12061 tccgtcgtcg tgttgtcggc gatcggactc gcttcgccgt aaccccgggt cgtcagccgt
    12121 tcgggcgcaa cgccctgggc gatcagctgc gcgcggacgg cctcggcgcg cttgtgcgac
    12181 aacgccaggt tgtagccgtc gttgccactg ctgtcggtga acccgccgac ttcgacggga
    12241 acctccggcg acgcgagcaa cgaggccgcc acgtcgtcga ggatctcctt cgagttcaac
    12301 gtgagctccg cgctgttcac ctcgaagaac acgccgtcga gcacgagact cgcccgctcc
    12361 tcgaacagcg gtgccgcgac cgggcagccg tcggggtcga ctcgcgcccc ggcggcggtc
    12421 tgcgggcagc ggtcgattcc gtcgtaaacc ccgtccgcgt cgctgtcgag cgggcagccg
    12481 tctgcgtcga cgtgggcgaa ctccggcgtg tcgggacagc ggtcgatccc gtcggcgacg
    12541 ccgtcaccgt caccgtcctg cgggcagccg cggtcgtcga ccgtgaccgc cggaggggtg
    12601 ccgggacagt cgtcctcacc gtcggtcacg ccgtcccggt cgctgtcgag cgggcagccg
    12661 gcgccgttga ccgcccaacc ggccggtgtg tcgggacact gatcgatccc gtcggcgacg
    12721 ccgtcgccgt cgccgtcctg cgggcagccc tgcgtgtcga cgcgcgcgcc gcgcggcgtg
    12781 tcgggacagg cgtccttccg gtcgggcacg ccgtcgccgt cgctgtccgc tgcgcggctc
    12841 ggcatgcccc agccgaagcc gacgagcaac aatccggtcg tcagggcggc gccgtccgcg
    12901 ccgtcgtcgg tcagcgtctg gtcgacacgc agctcccacc gcagcagctg gttcggcccg
    12961 acggtgaacc gctgtccgac gccgatcgag ccgaacaggc ggttgaagtc gtcgcgactc
    13021 tcacgggcga cgccgagcca gccgccgccg agcgcgacga acgtctgggc gtcggtccag
    13081 tgcggcgctc cgaacagctc gaccccgctg cgggcggcgt agttcgtgat cccgtcgccc
    13141 gctgccgact cgaagaccgc gagattcgag tcgacgaacc aaccccagcg tggagcgaac
    13201 tggaaggcac cgcggatccc cagggtcggc tcgagccgct cgagtgtcgt cggctcgccg
    13261 gtcaggtcgc gatccggcag cagcaaaccg gcctgcaagc cgatctcgcc cccctggcgc
    13321 gcggccgcgg cagcgggtcg cggtgccgcc ggtcccaccg cgaagagcat caaggcgacg
    13381 gcacaaacga gccggtggca gcccacgatc cacctcctcg attggatgtc gcggcgacga
    13441 tcctacagcc attcggcaaa tgcaagggga atcgggcgtc gcggctcaga cggtcgcctc
    13501 gtcgagcagt cgccagaagc tgtcgtagct ccgccccgtc gcgcgggtca gcccgatctc
    13561 gcaggtgcga ctggtgctgt aatagccgtc gtgacgtgcg cgattcacct cgcgcgcctc
    13621 gaccgcggtc gccgccgcgg tcagctcggg aacgcggtag ccccgatcgc cggcgaagcc
    13681 gcagcaaccg gaatcgatcg ggatctcgac ccgctcggcg aacggggcga cgacagcggc
    13741 ccaacgctcg accagaccca tcttcgacag cgagcagacc gggtgcagca cgaccgacgg
    13801 tttgggacgc gtcgggcgga gaccggccgc gagcagccgc tcggcgaact cgacgccgtc
    13861 gagaatctcg agcgcggcga aacgctcgcg gttggtcgcg tcgagctcgg gctcgcagct
    13921 cttcaacgtc agcgcgcagg ggctggtatc gagcacgatc ggcagctcgc cgccgccgct
    13981 ccagcgccac atccgctcga cgcagcgatt gacggccaag gcctgcgcgc ggcgatagcc
    14041 cttcgacgaa aacggcatgc cgcagcaggt gccggcgacg tcgccgggga tccggaccgg
    14101 tcgtccggcg cgggcggcga ccgcgaccag ccgcgtcgcc gatgattcga cgacgcgggt
    14161 gacgcagctc gggaaataga ccgccgtcgc accgccggcg cgcgtccgcg gcagaccgcg
    14221 gctcggccgc ggcagatcgc gccgcgcgag acggacggcc gacaatgccg cgcggaccag
    14281 ccgctcgacc cacgccagac gcagcgcgcc gagcgccgcc agacgctggg ccagcggcga
    14341 gtgcgcctcg gcgcgcagcc gcttcaccag cgcgcccgtg tcgatcgcga cggggcaggc
    14401 caacgcgcac agcccgtcgg cagcacaggt gtcgagcgcg tcgtagacga agtcccgctc
    14461 gagctcggcc agctccgccg agcgatccgc ctcggcccgc agccgcgcca tctcgcgacg
    14521 gacgacgatt cgctggcgcg gcgtcagcgt cagcgtgcgg ctcgggcaca tccgctcgca
    14581 gaaaccgcac tcgatgcagc ggtcgacctc ggcttcgacg gtcgccagcg tcttgaggtg
    14641 ccggatgtgg gcgcgcggat cgtcgttgag cagcacaccg gggttgagca ggccgcgcgg
    14701 gtcgaccagc ctcttgatct cgcgcatcac gcggtaggca tcggtgcccc attccgcttc
    14761 gacgaacggc gccatgttgc gtccggtccc gtgctccgcc ttgagcgcgc cgtcgtagcg
    14821 accgacgacg acctccgcca gcgcgtgcat gaacgcgtcg tagcgctcga cgtcgcgcgg
    14881 atcgtcgaac gcctgcacca gcacgaagtg cagattgccg tccttggcgt gcccgaagac
    14941 gatcgcgtcg tcgtaggcga agcgttcgaa caacgcgcgc agatcggcca cgccccgcgc
    15001 cagctgcggc acggggaaga cgatgtcctc gagaatgaac gaggttccac gccgtcgcat
    15061 cgccccgacc gacgggatca accccttgcg tacccgccac agcgccgccc gcggcaccgg
    15121 atcggtgctg agctcggcgg ggcggagcag cgacagccgc tcgagcaccc gccggcaggc
    15181 ttcgcggcgc tcgtcgagct cggcccgcga tccgcattga tactcgacca gcaacgccgc
    15241 agcgcccggc ggcagcccgc ggatcgccgg cggtacgccc ggctcgtgct cgatcgatcg
    15301 cagcgaggcg cgatccatca gctcgagcgc ctcggcaccc gagcgacgca gctcgtcgat
    15361 cgcggcgcag gcctgctcga cgccgcggaa cagcagcagc ccggtgtact tcagcgcgtg
    15421 gtcgggcacg gtgcgcagcg cggcgtcggc gatgaagccg agcgtgccct cggatccgat
    15481 catcaggtgg gacaggatct cgacggcgct gtcgtgatcg agcaacgcgt tcaacgagta
    15541 gccgttggtg ttcttcatct cgtacttggc gcggattctc gcggcgagcg cggagtcgcg
    15601 gcgcacgcga cggctcagct cgacgagtcc gtcggcgagg gccggtgact cgcgccgcag
    15661 gcgctcgtcg gcgtcgggcg ccgcgctgtc gatctcgacg ccgtcgggaa gcaggaagtg
    15721 gatcgactgc agcgtgtggt aggcgttccg ctcgacgccg cagcacatcc cgctcgagtt
    15781 gttggcgagg ataccgccca tcatgcaggc gttgatcgag gccgggtcgg ggccgatctt
    15841 ggtgccgtac ggttcgaggt gccggttgac ggtgccgccc agtacgcccg gctggaccca
    15901 caccgcgcgg ccgtgctcgc gcacctcgat cgcgtcccaa tggcgcgaga gcacgacgag
    15961 caggccgtcc gagacggcct gacccgacag gctggtgccc gccgagcgga aggtcaacgg
    16021 gatcccgcgg cgatgactga acgcgaacag ctgccgtacc tcgtcgagcg atcgcggaaa
    16081 gaccaccgcc tgcggcacca gccgatagac gctggcgtcg ttggcgtagg cgacacgatc
    16141 gatcagccgg gtcaacacgc gtcccggttc gagcatcgcg ctcaactcgc ttcgcacgcg
    16201 tccatcatcc cttgacgacc tctttggtcg caatagcggc gttgagcggt gcgcaaaggt
    16261 cggccgacaa tgggcgagga ggtgcgccat gccgacctac gtgttgatga ctcgtctcgc
    16321 gcccgactcg ctccacgacg cccggggccg ccgcgccgtc ggcaagagct ggctggaaca
    16381 ggtgcgcaag gcctgccccg acgtcgaatg gctggcccac tacgcgatcc tcggccccta
    16441 cgacttcatg gacatctacc gggcgcccga cgaggagacg gcgcacaaag tgtcgttgat
    16501 ctcgcgttcg gccggcgcgg tcaccgccga gagctggggc gcgctgcctt acgaccgcta
    16561 cctgttgctg ctcgagcagg tgcggcccga ctgacggttc cgtcatcatc gtcacggaaa
    16621 tgacgatgga acgcggccgg cccgtcgcgc catattccta tcaatcacca cggcgtctgg
    16681 tcggggaacg accgggaggt gcgcgatgct ggtgcgcgtg gcggaggaga gcttcgacaa
    16741 gggcgtcgcg gcgctcggcg acggtcgcca gcacgaggcg ttggccttct tcgaagcggc
    16801 gatcgagctc gagtgcaagt acggcacggg caagccgcag gcgcgctacc tctcccacta
    16861 cggcctctgc ctggggctcg ccggacgccg caagcacgaa gcggtcaagt tctgccggga
    16921 agcggtcgcg atggaggggt acaaccccga tctgctgtgg aacctgggcc gcgcgctgtt
    16981 gatcgccgat ctgcggcgcg aggcctacgc cgcgttcgtc aagggactgc gtctcgagcc
    17041 cggccacaag ggcatcatcc aagacctcaa ggcgatgggc ctgcgcaagc ggccgccgtt
    17101 gccgttcctc gatcgcagca atccgatcaa cgtgctgctc ggtcgcatcc gctcgaacgg
    17161 ctcctgaacg tcacccggcc agccggcgcg tcgagaacga acggggcgcg ttgtatgctt
    17221 tgcccgtgtc agcccagcga cgtctggccg tgctacccgt cgacgacgcg cgcggcgcca
    17281 gcacgcgcta ccgcgtgctg gcctacctgc cagcattgcg cgcggccggt ttcgacccgc
    17341 acgtccacga tccgctccgc acccgcggca cgctcgccgg cgccgcgcgt atcgccgatt
    17401 tgacacggga cgtcgcggcc gccagccgcg ccgaagtcgt gctcgttcac cgcaagatgt
    17461 atccgccgcc gttcgccggg cggctgcgac gcgccgcctc acggctggtg ctcgatatcg
    17521 acgatgcggt cgatctgccg ccccccggcc gtgagctcga ccgcaggacg cgcgagcggt
    17581 accgtcgcaa cttcgacgcg accgccgccg cggccgatct cgtcgtctgc ggcaacgcgg
    17641 agctcgagcg ccgcgtgccg cacgagcgca cggtgcggct gccgacgccg gtcgacaccg
    17701 cgcgtttccg ccccgacgcc ctcggcccgc cgcgaccgct cacgctcggt tgggtcggcc
    17761 actcggacaa cctgcgttac ctggagcggc tgttgccggt gctgcgcgag ctcgccggtc
    17821 gtcatcccgc gttctcgctg atcgtcgtcg ccgatcgtcc gccggccatc gacggcgtgc
    17881 cgatcgagtt ccgccgctgg tcgctcgagc aagagatctc gtgcttcgac ggcatcgcgg
    17941 tcggcttgat gccgctcgag gacggcgagt gggcgcggtc gaagtgtgcg ttcaaggcga
    18001 tccagtacat ggcgctcgga atcccgccgg tcgtctctcc cgtgggcatg aaccgcgagg
    18061 tcgtcgagca cggcatctcg ggcttcctcg ccgccgacgc cggccagtgg atcgaggcga
    18121 tcgagacgct gttctcgcgg cccgagctcg cggcgtcgat cggcgcggcc ggccgccgcg
    18181 tcgtcgagcg gcgctactcg ctcgcggcga acgcgccgtt gctgatcgac gccttgcgcg
    18241 gcgtcgggcg cacggcccgc tgctagaatc gccctcccga tgtgcggcat ctgcggaatc
    18301 ctcgactggg gacgcgacga cccggcggct cggcggaagc ggatcgaccg catggtcgcc
    18361 gcgttgacgc accgcggacc ggacgagggc ggctggtggg aggcgccgca gc
//
LOCUS       DASPGN010000205        10991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_271156, whole genome shotgun sequence.
ACCESSION   DASPGN010000205 DASPGN010000000
VERSION     DASPGN010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10991
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271156"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VD788_05825"
     CDS             complement(<1..1210)
                     /locus_tag="VD788_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75821.1"
                     /translation="MRLRHRRTPVVAATLALTALAAAHAASARRPASPLDPRIQHRRG
                     GSVATLPRELASLDPSDPLRRGWEEFRARHGGDWSVYLDERTAMPTLVSGRGVSFGSG
                     EIHALDELDRRARRFLGEHAALLGDASELLELDLAASRRLREDHWQLVYRQRVDGVRV
                     ENARLDLHVVRGRLVMLGASQWGRPTTDGLPALESGPARALLDGYLGAERSALVAAAP
                     PELVLIAVDAAPADEPRAWSGPRGEGLAHLLVWRFRFDEIDAPAHWIGEVDAHDGSVL
                     AFYEATQQASVRGGVFPISTDGDCLTGGCEVAGFPLPFADYTETGTLVEFTDDYGNLA
                     CDASEAEFVTRLDGPYVRIVDACGPTSEPGSCADGVDLGLKHGTNCDVAPGSSAGNTA
                     AARTAFYHVNR"
     gene            complement(1356..1904)
                     /locus_tag="VD788_05830"
     CDS             complement(1356..1904)
                     /locus_tag="VD788_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75822.1"
                     /translation="MPVKIVAGILVVAATATLADWIWYTFGVRHSVTAGLVHGAALLT
                     VVGAALGASAGRLLRGLPIGTIAGVGGALAYYAFIVWIDDRPYGPAIPAAWIVMWLLL
                     AVLEGRWLRAPARRSWVEIAVRGGTAALLSGVTFYLTLSTLWGAPPAGGRNYAIQFTA
                     WAFAWAPGLAALAAGRAGRSVE"
     gene            2042..4261
                     /locus_tag="VD788_05835"
     CDS             2042..4261
                     /locus_tag="VD788_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV75823.1"
                     /translation="MHGDPARASRVFVGLAIALAVPGITAALEGQVIDKESGKPLANV
                     TLSVIGRTQTARSDAEGRFVLVPDPRPPFELLAILPGGGYVKPVLFEALPVEGPIVVS
                     IGTAVVESVTVTAGAAPGIHAAPASGTTVVSREDVRSRNSRTLTQALENVPGVRNVSE
                     GHAAVPAIRGLSQARTLLLIDGARVTAERRVGPSATFLDPFVVEGVEVARGPGAVAYG
                     SDAFGGVINARTRRPTPGGELSGVATGTLGIGVPEGRLGLEVETPLSDKGSVLLSGHY
                     REFGDYDSPEGEVLDSGAGDAGFLISYAQLVPGGLLSVAVQGDHGRDVERPRTDSDVV
                     RFYYPEESSLRVTAAYETGPVSGFDSTELSLFVGDYEIVTDQDRFATATNPRQIERAD
                     VAARDYGLRANAERHWNQTRFDFGLDLKGRFDLEAEDVTIDFDASGMQTQAASFATIE
                     DARRIDTGIYVSAEGAVTPVVALASGLRFDWVRSTNEGGYFGDLAVDNSEPSGYAALT
                     LGAFGGFSTTLQYSHGFRDARLSDRFFRGVTGAGFITGNPDLEAETSDQLDLALRYTA
                     RRWRPAIYVYDYHIDDLIERFEDPSQPDFFFFRNRGRAEIRGVELELESELPRGVTLL
                     LAGSVAEGEALDDGTPLDDISPENLLVQARKVLGSRGWVQLRAAWVGELDEPGPNEVA
                     LDAYTVVDAAAGWRFDSGLELQLFVRNLLDETYLLTADDRSPLAPGTSAALSMQFTF"
     gene            4368..5288
                     /locus_tag="VD788_05840"
     CDS             4368..5288
                     /locus_tag="VD788_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV75824.1"
                     /translation="MIRAGQALVAVSLAAAVAGSAAAADVERGARLFARSCAACHGTS
                     GKGDGPGARQLDPRPRDLTTRQYRYRSTPTGSLPLPADLERTIRDGLPGTSMPGYGEL
                     FSSDQLDDLIAFVYSLQPAAVLEDPLPAPLAIGPLPEATPERIRDGHALYLLSGCATC
                     HDVRGRGKGPSARTLVDESERPIRPPDLRHDPLERGRDPEDVVRILRTGLNGAPMPSY
                     DDAMLIARGDVSDVAGFTDDPDVRTMLEDFLRSSPTREEIDAMSESDRAALRDGRLAA
                     LAYYVLSLGRRQGACYWLFVERPEREPRSR"
     gene            5288..5704
                     /locus_tag="VD788_05845"
     CDS             5288..5704
                     /locus_tag="VD788_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75825.1"
                     /translation="MRRAARSVSLGMLLVAGCAGDGSRIDEMTSDGILPTLASIQDHV
                     FTPICTECHFPGGPGPMPLTDESVSFQNLVNVQSVEDPGLLRVNPGDADASYIVHKIE
                     GRGTIVGTRMPPPPRPALTAEQIAAIVEWIDSGAAP"
     gene            5701..6801
                     /locus_tag="VD788_05850"
     CDS             5701..6801
                     /locus_tag="VD788_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75826.1"
                     /translation="MRVPRLDRLVLVAGTLSALASGPALAEPAFAVRTGYTCSQCHAN
                     RTGGGMRTAFGSLYAQTILPARLLSFDEARGLLPADPDARLGYGGDARFRYAYLRPDD
                     ADDTSSIDVSQGNLYLELRLIPGRLGLYLDETVGPGGASARELFGLYGFRWANAWIKA
                     GKLLPAYGWRLPDDDSFIRQSTGFTYSAPDNGIEFGVEPGRFSAVLSLTNGAGGGSDD
                     DRTKKVNFRAEHRFRAFRLGVSAAHNDTAGTRTTWGGLFGGGNWGRLSLVLEGDRIET
                     RQEESRSERWVGYVEADLLVARGLTLKYVHDWLDPDADVDTDERTRDSLGFEYVPIPF
                     VQARLFVRSHDGPPQVQGARGERIELELHLFF"
     gene            complement(6798..8417)
                     /locus_tag="VD788_05855"
     CDS             complement(6798..8417)
                     /locus_tag="VD788_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HXV75827.1"
                     /translation="MTCSARTPVGIALLCGATFAALPVHSVAWAQGRFVVEPGSSAGH
                     ARWLGVDGDLVAFGSGAEVTLVRGGAPGSVIARIELDRVVDRAVLADSRLWITGPLPG
                     LASVALGREAPPSEELPLDPPVQGPLRLARADDHLFAVEDGLGLRVIRLPGHAAGGPH
                     ASHHRTAAAQEALLEIHETFSAIAVSGRRAWLAIEGGDLIVVDIGDRRSPRLEGRVRP
                     GFEAIDLAANGETLYALDAGGTMRVLERDAGGDYRTTDELSMPGTTELDVAGRAIRFA
                     AGDSGVTTARDVSAIAANVNISVGDSFFSPANVSINVGDTVTWLKPNTALQHNVESCD
                     GVSDPGVCGGTVAADGLFRSGNATTASFSFAHTFLASGQNRYFCVIHSFGGMSGQVDV
                     AGGGGGTPPPVPDGHSVPGSQVVVGRLDQAGSSLSVSWDTSCPDAVDYHIVYGFGSNL
                     PAALGGSFGTDGGVCAIGSTSPFVWSGVPDPTVDPSRLLWWLVLANDGKGTEGSWSPD
                     SSGVERTGPGPAGSSTECAILNKDTANSCGL"
     gene            complement(8546..9898)
                     /locus_tag="VD788_05860"
     CDS             complement(8546..9898)
                     /locus_tag="VD788_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75828.1"
                     /translation="MTRTAVAVGALAAVLLVLGLAVAYRVLEWATSPAIAFPLPQAPA
                     ETDPAGETHRGFLYGRITTLDGATYEGRLRFGVAQEAFWGDYFNGAKDENPWVADVPI
                     ERLPNQRRALEIFGVEIGHREHEIDLGRPFMARFGDIARIEARGGDVRVTLKSGTLFD
                     LDRFSASDFDDGVRVWDATRGVVDLDSLWIRTIELLPTASLADAAPYRLHGTVRTRQG
                     EFTGFVQWDREECVGTDELDGRTAEGELGLRFDTIRSIARRSRHSSLVTLLDGREIVL
                     SATREVGHGNRGVYVDDPRYGRVLISWDAFERIDFSPGGSGPAYDDFPRGRPLTGRVT
                     TRAGRLLAGRLVYDLDESETVETLDAPSGGVDYTVPFGLIASIAPSGCGERGGEGATV
                     ILHGGEELQLECTGDLGAGNAGMLIFPDGRSSPEYVPWAEVEQVEFDRPPATYPPLPG
                     "
     gene            10003..10464
                     /locus_tag="VD788_05865"
     CDS             10003..10464
                     /locus_tag="VD788_05865"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXV75829.1"
                     /translation="MAALRWTIAVVACLALLVPRPALALTPDERTRLEAEVRAAEDAF
                     AKTLADRDLAAFARFVADDAAFFGRDDVSRGKPAVVQSWSGFFEGSTPPFSWRSELAV
                     VLDSGTLAHSSGPVFGPDGERFGTFNSIWRREPSGEWKVVFDKGCAYCEGK"
     gene            complement(10788..>10991)
                     /locus_tag="VD788_05870"
     CDS             complement(10788..>10991)
                     /locus_tag="VD788_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75830.1"
                     /translation="GDGLIDYLLTGFDIAYVVAGTDLHGVAGKPLGSADDDAPPAPGR
                     ELLVDRRSADGLNLTDPAFRFGD"
BASE COUNT         1627 a         3919 c         3895 g         1550 t
ORIGIN      
        1 tgcggttcac gtgatagaag gccgtgcggg cggccgcggt gtttcccgcg gaggagcccg
       61 gcgcgacgtc gcagttcgtg ccgtgcttca gccccaggtc gaccccgtcg gcgcagctcc
      121 cgggctccga ggtcgggccg cacgcgtcga cgatccggac gtacggtccg tcgaggcggg
      181 tcacgaactc cgcttcgctc gcgtcgcagg cgaggttccc gtagtcgtcg gtgaactcga
      241 cgagcgtacc ggtctcggtg tagtcggcga acggaagggg gaaccccgcg acctcgcaac
      301 ccccggtcag acaatcgccg tcggtgctga tcggaaagac cccgccgcgt acgctcgcct
      361 gctgggtcgc ctcgtagaac gccaggaccg acccgtcgtg ggcgtcgacc tcgccgatcc
      421 agtgggccgg ggcgtcgatc tcgtcgaagc ggaagcgcca gaccaacagg tgcgcgagcc
      481 cctcaccccg tggacccgac caggcacggg gctcgtctgc cggggcggcg tcgaccgcga
      541 tcagcacgag ctcgggcggg gcggcagcca cgagcgcgga ccgctcggcg ccgagatagc
      601 cgtcgagcag cgctctggcc ggacccgact cgagcgcggg gaggccgtcg gtggtcgggc
      661 ggccccactg cgacgctccc agcatcacga ggcggccccg cacgacgtgc aggtcgagcc
      721 gcgcgttctc gacgcgcacg ccgtcgacgc gctggcgata gacgagctgc caatggtcct
      781 cgcgcagccg gcggctcgcc gcgagatcga gctcgagcag ctcggacgcg tcgccaagca
      841 acgcggcgtg ttcgccgagg aacctccggg cgcgccggtc gagctcgtcc agcgcgtgga
      901 tctcgcccga gccgaagctc acccctcgac ccgagaccag cgtcggcatc gcggtgcgct
      961 cgtccaggta gaccgaccaa tcgccaccgt gacgcgcgcg gaactcctcc caaccacgac
     1021 gcagcggatc ggacggatcg aggctcgcga gctcgcgcgg cagcgtggcg accgagcccc
     1081 cacgacgatg ctggatgcgc gggtcgagcg gcgaggccgg gcgccgcgcc gacgccgcgt
     1141 gtgccgcggc gagcgccgtc agagcgagcg tggcggcgac gaccggggtc cgtcgatggc
     1201 ggagcctcac cacgacctcc gtgctgacgg atggcggcga ccacgccccg atctcccgtc
     1261 gcacgacggt ttatagacga tacgggagga gttgcccgtc ggggaaaccc cgaatcccga
     1321 cggatttccg catgacgccg gctcgggtga aggggctatt ccaccgatcg tcctgcgcgg
     1381 ccggcagcca gggccgcgag cccgggggcc caggcgaagg cccacgccgt gaactggatc
     1441 gcgtagttgc ggccgcccgc gggcggcgca ccccacagcg tgctcagcgt gagatagaag
     1501 gtcacaccgc tgagcaatgc agccgtgccg ccgcggacgg cgatctcgac ccacgatcgg
     1561 cgggccgggg cgcgcagcca gcggccctcg aggacggcga gcagcaacca catcacgatc
     1621 caagcggccg ggatcgccgg accgtacggc cgatcgtcga tccacacgat gaacgcgtaa
     1681 taggcgagcg cgccgccgac gccggcgatc gtaccgatgg gcaacccccg gagcaggcgt
     1741 ccggccgatg cgcccagtgc cgccccgacg accgtgagca acgccgcgcc gtggaccagc
     1801 cccgccgtca cgctgtgacg tacgccgaag gtgtaccaga tccagtccgc cagtgtcgcg
     1861 gttgcggcaa ccaccaggat tcccgcgacg attttgaccg gcatgatccg acactccgcg
     1921 aacggcccgt tcgatcgagg ataggccgac gaggagcggg cgcgcaagtt cgagacgacc
     1981 tacgagtcct ggtagtagtc ccgcgtgcgt ggtctgatgc ggccgggggg gatcgggttc
     2041 gatgcacggc gatccagcga gagcctcacg cgttttcgtc gggctggcga tcgccctcgc
     2101 cgtacccggt atcacggccg cgctcgaggg ccaggtcatc gacaaggaga gcggcaagcc
     2161 cctggccaac gtcacgctct cggtgatcgg acgcacacag accgcgcgta gcgacgccga
     2221 ggggcggttc gtgctggtgc ccgacccgcg cccgcccttc gaactgttgg cgatcctgcc
     2281 cggtggcggc tacgtgaagc ccgtgctgtt cgaggcgctg cctgtcgaag gaccgatcgt
     2341 cgtctcgatc gggaccgccg tggtcgaatc ggtcacggtg accgcggggg cggcacccgg
     2401 aatccacgcc gcgccggcca gcgggaccac cgtcgtctcg cgcgaggacg tgcgctcgcg
     2461 gaactcgcgc acgttgaccc aggcgctcga gaacgtcccc ggggtgcgca acgtctccga
     2521 gggtcacgcg gcggttcccg cgatacgcgg gctgtcccag gcgcggacgc tgctcctgat
     2581 cgacggggcg cgcgtcacgg cggagcgccg cgtcggcccg agcgcgacct ttctcgaccc
     2641 gttcgtggtc gagggcgtcg aggtcgcgcg cggaccgggc gccgtggcct acggctcgga
     2701 cgccttcggc ggcgtgatca acgccaggac gcggagaccg acaccgggag gcgagctgtc
     2761 cggagtcgcg acggggaccc tcgggatcgg cgtgcccgag gggcggctcg gcctcgaggt
     2821 cgaaacaccg ctcagcgata aggggagcgt gctgctgtcg ggccactacc gggagttcgg
     2881 cgactacgac agccccgagg gcgaggtgct cgactccggc gccggcgatg cgggcttcct
     2941 gatcagctac gcgcagctcg tgcccggcgg tctgctctcc gtcgccgtcc agggagacca
     3001 cggccgcgac gtcgagcggc cccgcaccga ctcggacgtc gtgcgcttct actatccgga
     3061 agagagctcg ctgagagtca ccgcggccta cgaaacgggt ccggtctcgg gcttcgactc
     3121 gacggagctg tcgctgttcg tcggtgacta cgagatcgtc accgatcagg accgcttcgc
     3181 cacggccacg aacccgcgcc agatcgagcg ggccgacgtc gccgcgcggg actacgggct
     3241 gcgggccaac gcggaacggc attggaacca gacgcggttc gacttcggcc tcgacctcaa
     3301 gggccgcttc gacctcgagg ccgaggacgt gacgatcgac ttcgacgcct cgggcatgca
     3361 aacgcaggcg gcgtcgttcg cgacgatcga ggatgcccgc cgcatcgaca ccgggatcta
     3421 cgtctcggcc gagggcgcgg tgacgcccgt cgtcgcgctc gcctccggtc tgcgcttcga
     3481 ctgggtacgc tcgacgaacg agggcggcta cttcggcgac ctcgcggtcg acaactccga
     3541 gccgtcgggc tacgcggcgc tgaccctcgg cgccttcggc ggcttctcga cgacgctgca
     3601 gtactcccac ggcttccgcg atgcccgtct gtcggatcgg ttcttccgcg gtgtcaccgg
     3661 cgccggcttc atcacgggca accccgacct cgaagcggaa accagcgacc agctcgatct
     3721 ggccctgcgc tacaccgccc ggcgctggcg ccccgcgatc tacgtctacg actaccacat
     3781 cgacgacctg atcgagcgct tcgaggatcc gtcgcagccg gatttcttct ttttccgcaa
     3841 ccgcggtcgg gcggagatcc gcggtgtgga gctcgagctc gagtcggagc tgccgcgagg
     3901 cgtgacgttg ctcctcgccg gcagcgtggc cgagggcgaa gcgctcgacg acggcacccc
     3961 gctcgacgac atctcgcccg aaaacctgct ggtccaggcg cgcaaggtgc tgggctctcg
     4021 cggctgggtg cagctgcgcg cggcgtgggt cggcgagctg gacgagccgg gaccgaacga
     4081 ggtcgcgctc gacgcctaca ccgtcgtgga cgcggccgcc ggctggcgtt tcgattcggg
     4141 tctcgagctg cagctcttcg tgcgcaacct gctcgacgag acctacctgc tcaccgcgga
     4201 cgatcgctcg ccgctggccc cgggtacctc cgcggccctg tcgatgcagt tcacgttctg
     4261 aggcctggaa cttgccgcac gatccagcct gttccgaacg ggcgcgggag ctgtcccatc
     4321 ttcccccgac ctgtcgggga gggagaaact cgcgtgaggc gacccggttg atccgcgcgg
     4381 gacaggcgct cgtggccgtg tctctcgccg ccgccgttgc gggatcggcg gccgccgccg
     4441 atgtcgagcg cggagcgcgg ctcttcgccc ggtcctgtgc cgcgtgccac ggaacgagcg
     4501 gcaagggcga cgggcccggc gcgcggcagc tcgatcctcg gccgcgagac ctgacgacgc
     4561 gccagtaccg gtaccgctcc acgccgacgg gcagcctccc gctgccggcg gatctcgagc
     4621 ggacgatccg cgatgggctc ccgggcacct cgatgcccgg atacggcgag ctgttctcgt
     4681 ccgaccagct cgacgacctg atcgccttcg tctactcgct ccagccagcg gccgtcctcg
     4741 aggacccgct gcccgcgccg ctcgcgatcg gcccacttcc cgaggcgacg cccgagcgaa
     4801 tccgggacgg acacgcgctg tatctgctga gcggctgtgc gacctgccac gacgtgcgcg
     4861 ggaggggcaa gggcccgtcc gccaggacgc tcgtcgacga gtcggagcgc ccgatccgcc
     4921 cgccggatct gcgtcacgat ccgctcgagc gcgggcgcga tccggaggac gtcgtgcgca
     4981 tcctgcgcac cgggctcaac ggtgcgccga tgccgtccta cgacgacgcg atgctgatcg
     5041 cccgcggcga cgtgtccgac gtcgccggct tcaccgacga tcccgacgtc cggacgatgc
     5101 tcgaggattt cctgcgctcg agcccgacgc gcgaggagat cgacgcgatg tcggagtccg
     5161 atcgcgccgc gctgcgcgac ggtcgtctgg ccgcgctggc ctactacgtc ctgtccctcg
     5221 gccggcgtca gggggcctgc tactggctgt tcgtcgagcg accggaacgc gagccgcgga
     5281 gtcgctgatg cgtcgcgccg cacgctcggt ctcgctcggg atgctgctcg tcgccggctg
     5341 cgccggggac ggatcgcgca tcgacgagat gacgagcgac ggcatcctcc cgacgctggc
     5401 ctcgatccag gaccacgtgt tcaccccgat ctgcaccgag tgtcacttcc ccggtgggcc
     5461 ggggccgatg ccgctgaccg acgagtcggt ctcgttccag aacctggtca acgtccagag
     5521 cgtggaggat cccggcctgc ttcgcgtcaa cccgggcgac gcggacgcct cctacatcgt
     5581 gcacaagatc gaaggtcgcg ggacgatcgt cggcacccgc atgccgccgc cgccgcgccc
     5641 cgcgctgacg gccgagcaga tcgccgccat cgtcgagtgg atcgatagcg gtgccgctcc
     5701 gtgagggttc cccggctcga ccgactcgtg ctcgtcgcgg gcacgctgtc cgcgctcgcg
     5761 tcgggccccg cgctcgccga gcccgcgttc gccgtccgca ccggttacac ctgctcgcag
     5821 tgtcacgcga accggaccgg cggaggcatg cgaacggcat tcggcagcct ctacgcacag
     5881 acgatcctcc cggcacgcct cctctcgttc gacgaggcgc gagggctgct gccggccgac
     5941 cccgacgcgc ggctcgggta cgggggcgac gcgcgattcc gctacgcgta cctgcggccc
     6001 gacgacgccg acgacacctc gtcgatcgac gtgtcccagg gcaacctcta cctcgagctg
     6061 cggctgatcc cggggcgcct cggcttgtac ctcgacgaga ccgtcgggcc gggtggagcc
     6121 tcggcgcgag agctgttcgg actgtacggc tttcgctggg ccaacgcatg gatcaaggcc
     6181 gggaagctcc tcccggccta cggctggcgg ctgcccgacg acgacagctt catccggcaa
     6241 tcgaccggct tcacctacag cgcgcccgac aacgggatcg agttcggggt cgagcccgga
     6301 cgcttcagcg ccgtgctgtc actcacgaac ggcgcgggcg gcgggagcga cgacgaccgg
     6361 accaagaagg tcaacttccg cgccgagcat cgcttccgcg cgttccggct cggcgtgtcg
     6421 gccgcgcaca acgacacggc cggaacgagg acgacctggg gcgggctttt cggtggcggc
     6481 aactggggcc ggctctcgct cgtgctcgag ggcgaccgga tcgagacgag gcaggaggaa
     6541 tcgcggagcg agcgctgggt cgggtacgtc gaggccgacc tgctcgtcgc ccggggcctg
     6601 accctgaagt acgtccacga ctggctcgat cctgacgccg acgtcgacac cgacgagcga
     6661 acccgcgact cgctgggctt cgagtacgtc ccgatcccgt tcgtgcaggc ccgcctgttc
     6721 gtccggagcc acgacgggcc cccgcaggtc cagggggcgc gcggggagcg gatcgagctc
     6781 gagctgcacc tgttcttcta gagtccgcag ctgttggcag tgtccttgtt gaggatcgcg
     6841 cactcggtcg aggatcccgc ggggccgggg ccggtacgtt cgacgcccga gctgtccgga
     6901 ctccaggagc cttccgtccc cttcccgtcg ttggccagga cgagccacca cagcaggcgg
     6961 ctgggatcca cggtgggatc cggcacaccg ctccacacga acggcgaggt cgacccgatc
     7021 gcgcacacgc cgccgtcggt cccgaacgac ccgccgagtg cggcgggcag attcgagccg
     7081 aagccgtaca cgatgtggta gtcgacggcg tccggacagc tcgtgtccca cgagaccgac
     7141 agcgacgagc ccgcctggtc gagccgcccg acgacgacct gggagcccgg aacgctgtga
     7201 ccgtcgggaa ccggcggagg agtgccgccg ccgccgccgg ccacgtcgac ctgcccgctc
     7261 atccccccga aggagtggat cacgcaaaag taccgattct gccccgaggc gaggaaggtg
     7321 tgcgcgaagc tgaacgacgc ggtcgtcgcg ttgccggagc ggaacagccc gtccgccgcc
     7381 accgtccccc cgcagacccc cggatcgctc acgccgtcgc aggactcgac gttgtgctgg
     7441 agcgcggtgt tgggcttcaa ccaggtcacg gtatcgccga cgttgatcga cacgttggcc
     7501 ggcgagaaga agctgtcgcc gaccgagatg ttgacgtttg cggcgatcgc cgagacgtcg
     7561 cgcgcggtgg tcacgccgga gtcgcccgcg gcgaaacgga tcgcgcgtcc cgccacgtcg
     7621 agctcggtcg tgcccggcat actcagctcg tcggtcgttc ggtagtcacc gccggcgtcg
     7681 cgctcgagca cgcgcatcgt gccgcccgcg tcgagcgcgt agagcgtctc gccgttcgcc
     7741 gcgaggtcga tcgcttcgaa gcccggacgg acgcgaccct cgagccgcgg gctgcgccgg
     7801 tccccgatgt cgacgacgat caggtcgcca ccctcgatgg cgagccaggc ccgacggccc
     7861 gagaccgcga tggccgagaa ggtctcgtgg atctcgagca gcgcctcctg cgccgcggcg
     7921 gttcgatggt gggaggcgtg cggaccgccc gcggcgtggc ccgggagccg gatgacacgc
     7981 agccccagac cgtcctcgac ggcgaacagg tgatcgtccg cccgcgccag acgcagtggt
     8041 ccctgaaccg gtgggtcgag cggaagctcc tcggacggcg gcgcctcgcg cccgagcgcg
     8101 accgaggcca ggcccggcaa cggtccggtg atccacagcc gggagtccgc gagcaccgcc
     8161 cggtccacca cgcggtcgag ctcaatgcga gcgatgaccg agccgggagc gccgccccgc
     8221 acgagcgtga cctcggcgcc cgagccgaag gcgacgagat ccccgtcgac gccgagccag
     8281 cgggcgtgac ccgcgctcga gcccggctcg acgacgaatc gcccctgggc ccacgctacc
     8341 gagtggacgg ggagcgccgc gaacgtcgcg ccgcaaagca gtgcgatccc gaccggggtc
     8401 cgtgcagagc aagtcatgtt ttcggtctcc acccaggccg cgggccccgg cgaccttggg
     8461 ctccagcgcc cgcgatcgcc ggtggtccgg gagggagtac tgggacagcc gccgaccccc
     8521 gatggaacat ccgaatcgga ggtggctagc cggggagcgg ggggtaggtc gccggcgggc
     8581 ggtcgaactc gacctgctcg acctcggccc agggaacgta ctccgggctt gaacggccgt
     8641 ccgggaagat cagcatgccg gcattccccg caccgagatc gccggtgcac tcgagctgca
     8701 gctcctcccc gccgtggagg atcaccgtgg cgccttctcc gccgcgctct ccgcaaccgg
     8761 agggggcgat cgaggcgatc aggccgaacg ggacggtgta gtccacgccc ccggacgggg
     8821 cgtcgagcgt ctcgacggtc tcgctttcgt cgaggtcgta gaccagccgg ccggcgagaa
     8881 ggcggccggc acgggtggtg acgcgacccg tgagcgggcg cccccgcggg aagtcgtcgt
     8941 acgccgggcc gctcccgccg gggctgaagt cgatccgctc gaaggcgtcc cacgagatca
     9001 gcacccggcc gtagcgtggg tcgtcgacgt agacgccgcg gttgccgtgg ccgacttcgc
     9061 gggtggcgga gagcacgatc tcgcgtccgt cgagcagcgt cacgagggaa ctgtggcgcg
     9121 agcggcgcgc gatcgagcgg atggtgtcga aacgcagacc gagctcaccc tcggcggtgc
     9181 ggccgtcgag ctcgtccgtg ccgacgcact cctcccggtc ccactggacg aagccggtga
     9241 actcgccctg ccgtgtgcgc accgtaccgt gcagccggta gggggcggcg tccgccagcg
     9301 aggcggtcgg gagcagctcg atcgtgcgaa tccacaggct gtcgaggtcg acgacacccc
     9361 gcgtcgcgtc ccacacccgc acgccgtcgt cgaagtcgct ggcggagaag cgatcgaggt
     9421 cgaacagggt cccgctcttg agagtcacgc gcacgtcgcc gccgcgcgcc tcgatgcgcg
     9481 cgatgtcgcc gaagcgcgcc atgaacggcc ggccgaggtc gatctcgtgc tcccggtggc
     9541 cgatctcgac cccgaagatc tcgagcgcgc ggcgctggtt gggcaaccgc tcgatcggca
     9601 cgtcggcgac ccaggggttt tcgtccttgg cgccgttgaa gtagtcgccc cagaacgcct
     9661 cctgggcgac accaaagcgc agccgcccct cgtaggtcgc gccgtcgagc gtggtgatcc
     9721 tgccgtagag gaacccccga tgggtctccc ctgccgggtc ggtctccgcc ggtgcctgcg
     9781 ggagtgggaa cgcgatcgcg ggcgatgtcg cccactcgag gacgcggtag gcgacggcca
     9841 gaccgaggac gaggagcacg gcggcaagcg ccccgacggc gacagcggtt ctcgtcactg
     9901 aacctccttc ggggtgacgc cagtctacct cgggtcccgc cgaaccccga ctctccgccc
     9961 gcgtatgatg atcatcatgg atccgaccga cgaggtgcac tcatggctgc tctacgttgg
    10021 acaatcgccg tcgttgcctg cctggcgctg ctcgtcccgc gaccggcgct cgcgctgacg
    10081 cccgacgagc ggacccggct cgaagccgag gtgcgcgcgg ccgaggatgc cttcgcgaag
    10141 acgctcgccg atcgcgacct ggccgcgttc gcccgcttcg tcgccgacga cgcggcgttc
    10201 ttcgggcgcg acgacgtctc gcgcggcaag ccggccgtcg tgcaatcgtg gagcggcttc
    10261 ttcgagggat cgacccctcc gttctcgtgg cggtcggagc tggccgtggt gctcgactcc
    10321 gggacgctgg cccactcgtc gggaccggtg ttcggtccgg acggcgagcg cttcgggacc
    10381 ttcaactcga tctggcggcg cgagccgagc ggcgagtgga aggtcgtctt cgacaagggt
    10441 tgcgcctact gcgaagggaa gtagcgcaga cggcgattcg cagcggagga tacgcatgag
    10501 agcactcgta ctgctgatga tgctcggtcg tccctccgga ggcggccgtc gctctggcgt
    10561 gagcttgcct ccgacctgtc gctgacggcg gagcagcggc agcgcgtcga cgcgattcgc
    10621 gccgagatgc tggaaggggc gaggcggatc ggcaccgagt tggtggaagc ggagcgcttg
    10681 ctacgacgaa ctgcgtggat acacccgctg accgctctcg cccacgagcg gcttcgccgc
    10741 gcggcgggac ggcgcccgtc gggccgtccc gccggtcccg ttgaacgcta gtcgccgaag
    10801 cggaaagccg ggtccgtcag gttgagtccg tcggccgaac gccggtcgac gagcagttct
    10861 cgcccgggtg cgggcggcgc gtcgtcgtca gcggagccga gcggttttcc ggcgacgccg
    10921 tgcagatcgg tgcccgccac cacgtaggcg atgtcgaaac ccgtcagcaa atagtcgatc
    10981 aggccgtctc c
//
LOCUS       DASPGN010000206         4874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_271326, whole genome shotgun sequence.
ACCESSION   DASPGN010000206 DASPGN010000000
VERSION     DASPGN010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271326"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1558)
                     /locus_tag="VD788_05875"
     CDS             complement(<1..1558)
                     /locus_tag="VD788_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXV75831.1"
                     /translation="MATVDNGNRPLRTGWASWLATTAGGLLLFEVVSGLAVTLAPFHP
                     VVEWGVLLHTLVGALTLLPLGWYLAAHWLDYKHFALSHVVLLGYVATVSLAVCSVSGL
                     VVTFEGLLALRISTLWRQIHLVSTLVTLVAVLPHVVLVLLRLRGAERRPAWRVVGVGS
                     GGAAAGLIAVAALATLYSGTAYVNEFPADYAWIYGRERPFAPSLARTASGGAYDPRSL
                     AGSASCGTSGCHEQILAEWRPSAHRWAAMDTAFQKIQSIMAEQNGAESTRYCGGCHDP
                     ISLFSGTKNIGVETLTGLLGHQEGVSCLVCHAIRETDLQGNANYVLAQPREYLWQWAD
                     DGPRRLLRDFLIRTYPDEHARLSKRAYKAPEYCAACHKQFIDQEVNRVGWVQLQNQYD
                     NWKASHWNVPGDARRTVECRECHMPLVDSRDPAAGDAADYNRRPDDGRHRSHRFVAAN
                     AMMPALLQVDGWQRQAELTEAWLAGRLEIPEIADKWASGPIVTIALETPAAVAPGDRF
                     DVRVVLTSNKV"
     gene            complement(1651..>4874)
                     /locus_tag="VD788_05880"
     CDS             complement(1651..>4874)
                     /locus_tag="VD788_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV75832.1"
                     /translation="PPDAWDVNNPPGVWRTIGIDTEETTWSDVDVSPDGKTIVFDMLG
                     DVYAVPIGGGQARALTSGIAWDQQPRFSPDGSRIAFISDRAGGDNLWLIDADGANPRA
                     VTEEKEHLVHNPAWSPDGRYIVAKKGFTSTRSIPAGEIWLFHIGGGGGLQITERPHKE
                     QDQKNMAEPAFSPDGRFIYYSQDATSGRVWQYNKDSTGEIFVIKRLDRAEAKTDVVVS
                     GPGGAIRPTPSPDGKYLAFVKRLPGLHSALYLKDLDSGKEWAIYRRMERDLQETNGSE
                     GNAPGFAWTPDSRSIVFWAEGRIRRIDVDSREVALVPIRVKTEMKVRQALRFPVDVAP
                     DEFPVRMLRWAQLSPTGETALFQALGQLYTAGADGRGRKRLTDQHEHYEFWPSFSPDG
                     RQLVYTTWSDSELGSVRVATLDGSTSRSVTPAPGHYVEPRFSPDGSMVAYRKITGGYL
                     LSPLWSEEPGIYVVAATGGGEPKRVSRSGSNPQFSADGKRLFFTDVVEETQAVLKSVD
                     LAGNDERTHLQGKQVTEFSVSPDGRWVAFTENHNAFVAPLTATGKKVEIGAETKSVPV
                     KQVSQRSGEFLHWSADSSTLHWSHGATLYSRVLADAFGFLEGAPDELPEPVASGIDLG
                     FDTAADKPSGLIALTGARIVTMRDAGQRREVIDDGVVLVEGNRIRSVGAAAEIDVPHD
                     ALVIAVSGKTIIPGLVDVHAHGPFANSEITPRQNWTQYSNLAFGVTTIHDPSNDTTEV
                     FAASELQRAGLVVAPRIYSTGTILYGAHVPGYTAKIDSYDDAEFHVRRLKETGAISVK
                     SYNQPRRDQRQQVIQAGAELGVMVVPEGGAKFQHNMTQIVDGHTGIEHAIPLARGYDD
                     VVQLWSQTETGYTPTFGVAYGGLAGEVYWYDRTKVWENERLMRYTPRFVVEPVSIRRT
                     TAPDDHYNHIEVARFAKKLRDAGVSVQIGAHGQREGLAAHWEMWMMEQGGFTPWEAIR
                     AATIDGAAYVGLDRDVGSVEPGKLADLVVIDGNPLEDLRASERVLYTMINGRLYEAAT
                     MNQIAPDRVERGPFFFELEGGDTVHAATQQWIETMSRRYGWRH"
BASE COUNT          731 a         1668 c         1659 g          816 t
ORIGIN      
        1 ccaccttgtt cgacgtcagc acgacgcgca cgtcgaagcg gtcgccgggg gcgaccgcgg
       61 ccggcgtctc gagcgcgatg gtgacgatcg gtccgctggc ccacttgtcc gcgatctcgg
      121 gtatctcgag ccggccggcg agccaggcct cggtcagctc ggcctggcgc tgccagccgt
      181 cgacctgcag cagggccggc atcatcgcgt tcgctgcgac gaatcggtgg ctgcggtgcc
      241 taccatcgtc gggccggcgg ttgtagtcgg ccgcgtcgcc ggccgccggg tcgcgcgagt
      301 cgaccagcgg catgtgacac tcgcggcact cgaccgtcct gcgagcatcg cccggcacgt
      361 tccagtggct cgccttccag ttgtcgtact ggttctgtaa ctgcacccag cctacgcggt
      421 tcacttcctg atcgatgaac tgcttgtgac aggccgcgca gtattcggga gccttgtagg
      481 cccgtttgct cagtcgggcg tgctcgtcgg ggtaggtccg gatcaggaag tcgcgcagca
      541 ggcgtcgcgg tccgtcgtcg gcccattgcc agaggtactc cctcggctgg gccagcacgt
      601 agttcgcgtt tccttgcagg tccgtctcgc gaatggcgtg gcagaccaga cacgagacgc
      661 cctcctggtg accgagcagc ccggtcaggg tctcgacgcc gatgttcttg gtccccgaga
      721 acagcgagat cgggtcgtgg caacccccgc aataccgtgt ggactcggca ccgttctgct
      781 cggccatgat gctctggatc ttctgaaacg cggtatccat cgccgcccaa cggtgagcgc
      841 tcgggcgcca ctcggcgagg atctgctcgt ggcacccgct cgtcccgcag ctggccgacc
      901 cggccagcga gcgaggatcg taggccccac cgctggccgt gcgtgccagg ctgggagcga
      961 acggccgctc gcggccgtag atccacgcgt agtccgccgg gaactcgttg acgtaggccg
     1021 tgcccgagta gagggtggcg agcgccgcga cggcgatcaa cccggccgcc gcgccgccgg
     1081 agccgacgcc gaccacgcgc cacgccggtc gccgctcggc cccgcgcaag cgcagcagca
     1141 ccagcacgac gtgcggtagc acggcgacca gcgtgacgag cgtcgagacc aggtggatct
     1201 gccgccagag ggtcgatatg cgcagggcga gcaggccctc gaacgtcact acgaggcccg
     1261 acaccgagca gaccgcgagc gacaccgtcg cgacgtagcc gagcagaacg acgtgcgaca
     1321 gagcgaagtg cttgtagtcg agccagtgcg ccgccaggta ccagccgagt ggcagcagcg
     1381 tcagcgcccc gacgagcgtg tggagcagca cgccccactc gacgaccggg tggaacggtg
     1441 ccagcgtgac cgccagaccg ctcacgacct cgaacagcag gaggccaccg gcggtcgtgg
     1501 ccagccacga agcccagccg gtacgcagcg gccggttccc gttgtcgacc gtagccatcc
     1561 acttctcccc gacgaatgga ggagatgtac ggagagtcgg ctctccacgg gagcgccggc
     1621 agcggcctaa agagaccgga attgtccggt tcagtgtctc cagccgtagc gacggctcat
     1681 cgtctcgatc cactgctgcg tcgcggcgtg cacggtgtcg ccgccttcca gctcgaagaa
     1741 gaacgggccg cgctcgaccc gatcgggtgc gatctggttc atcgtcgcgg cctcgtacag
     1801 gcgcccgttg atcatcgtgt acagcacgcg ttccgacgcg cgcagatcct cgagcggatt
     1861 gccgtcgatc acgaccagat cggcgagctt gcccggctcg accgaaccga cgtcgcgatc
     1921 gagaccgacg taggccgcgc cgtcgatcgt tgcggcgcgg atcgcctccc atggggtgaa
     1981 gccgccctgc tccatcatcc acatctccca gtgggccgcg aggccttcgc gttggccgtg
     2041 cgcgccgatc tgcacgctga cgccggcgtc gcgcagcttc ttggcgaaac gcgccacctc
     2101 gatgtggttg tagtgatcgt ccggcgccgt cgtgcgacga atcgacaccg gctcgacgac
     2161 gaaccgcggc gtgtagcgca tcaagcgctc gttttcccag actttggtgc ggtcgtacca
     2221 gtagacctcg ccggccagcc cgccgtaggc cacgccgaag gtcggcgtgt agccggtctc
     2281 ggtctgggac cacaactgga ccacgtcgtc gtagccgcgc gccaacggta tggcgtgctc
     2341 gatcccggtg tgaccgtcga cgatctgcgt catgttgtgc tggaacttgg ctccgccctc
     2401 gggcacgacc atcacgccga gctcggcgcc ggcctggatc acctgctgcc gctggtcccg
     2461 gcgcggctgg ttgtagctct tgaccgagat cgcgcccgtc tccttcagcc gacgcacgtg
     2521 aaactcggcg tcgtcgtacg agtcgatctt ggcggtgtag cccggtacgt gcgctccgta
     2581 caggatcgtt cccgtcgagt agatccgcgg cgcgacgacg agcccggcgc gctgcagctc
     2641 ggaagccgcg aagacctccg tcgtgtcgtt ggacgggtcg tggatcgtcg tcacgccgaa
     2701 cgcgaggttc gagtactgcg tccagttctg ccgcggcgtg atctcgctgt tggcgaacgg
     2761 gccgtgggca tggacgtcga ccaggccggg gatgatcgtc ttgccgctga cggcgatcac
     2821 cagggcgtca tgcgggacgt cgatctccgc agccgcaccg accgagcgaa tgcggttgcc
     2881 ctcgaccagc acgactccgt cgtcgatcac ctcccggcgc tggcccgcat cgcgcatggt
     2941 gacgatgcgt gcgccggtca gcgcgatcag tccggacggc ttgtcggccg ccgtgtcgaa
     3001 gccgaggtcg ataccgctcg cgaccggctc gggtagctcg tccggggcgc cctcgagaaa
     3061 gccgaacgca tcggccagca cgcgactgta gagggtcgca ccgtgcgacc agtggagcgt
     3121 cgagctgtcg gccgaccagt ggaggaactc gcccgagcgt tgtgaaactt gcttgaccgg
     3181 gaccgacttg gtctccgcac cgatctcgac tttcttcccg gtcgcggtca gcggcgcgac
     3241 gaacgcgttg tggttctcgg tgaacgcgac ccagcgcccg tccggcgaaa cgctgaactc
     3301 ggtcacctgc ttgccttgca gatgggtgcg ctcgtcgttg ccggcgaggt cgacgctctt
     3361 cagcacggcc tgggtctctt cgaccacgtc ggtgaagaac agacgcttgc cgtcggcgga
     3421 gaactgcggg ttcgagccgc tgcgcgagac gcgcttcggt tcgccgccgc cggtcgccgc
     3481 gacgacgtag atgcccggct cctcggacca cagcggtgac agcaggtagc ctcccgtgat
     3541 cttgcggtag gcgaccatcg agccgtccgg cgagaagcgc ggctcgacgt agtggccggg
     3601 agcgggggtg acgctgcgcg acgtcgagcc gtcgagcgtc gcgacccgga cgctgcccaa
     3661 ctcggaatcg ctccacgtcg tgtagaccag ttggcgaccg tccggcgaga acgagggcca
     3721 gaactcgtaa tgttcgtgtt ggtcggtcaa ccgcttccgc ccgcggccgt cggcaccggc
     3781 cgtgtagagc tgaccgagcg cctggaacaa tgccgtctcc ccggtgggcg agagctgcgc
     3841 ccagcgcagc atccgcaccg ggaactcgtc gggcgcgacg tcgaccggga aacgcagcgc
     3901 ctggcgcacc ttcatctcgg ttttgacgcg gatcgggacc agcgcgacct cgcgcgagtc
     3961 gacgtcgatg cggcggatcc tgccttcggc ccagaagacg atcgagcgcg agtcgggcgt
     4021 ccaggcgaaa cccggcgcgt tgccctcgct gccgttggtt tcctgcaggt cgcgctccat
     4081 gcggcggtag atcgcccact ccttgcccga gtcgaggtcc ttcaagtaca gcgcgctgtg
     4141 gaggccgggc agccgtttga cgaacgccag gtacttgccg tcgggggacg gcgtgggccg
     4201 gatcgcgccg ccgggcccgc tgacgacgac gtcggtcttc gcctcggctc gatcgagcct
     4261 cttgatgacg aagatctcac cggtcgagtc tttgttgtac tgccagaccc gaccggaggt
     4321 cgcgtcctgg ctgtagtaga tgaaccgccc gtccggcgag aacgccggct cggccatgtt
     4381 cttctggtcc tgctctttgt gggggcgctc ggtgatctgc aagccgccgc cgccgccgat
     4441 gtggaacaac cagatctcac cggcgggaat gctgcgcgtc gaggtgaacc ccttcttggc
     4501 cacgatgtag cggccgtcgg ggctccacgc ggggttgtgc accaagtgct ccttctcctc
     4561 ggtgaccgcg cgcgggttgg ccccgtccgc atcgatcagc cacaggttgt cgccgccggc
     4621 gcgatcgctg atgaacgcga tgcggctgcc gtccggactg aatcgcggct gctggtccca
     4681 ggcgatcccg ctcgtgagcg cgcgggcttg accgccgccg atcggcaccg cgtagacgtc
     4741 gcccagcatg tcgaagacga tcgtcttccc gtccgggctg acgtcgacgt ccgaccacgt
     4801 ggtctcctcg gtgtcgatcc cgatcgtgcg ccagacgccc ggcgggttgt tcacgtccca
     4861 cgcgtcgggg gggg
//
LOCUS       DASPGN010000207         4598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_856267, whole genome shotgun sequence.
ACCESSION   DASPGN010000207 DASPGN010000000
VERSION     DASPGN010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4598
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856267"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="VD788_05885"
     CDS             complement(<1..1385)
                     /locus_tag="VD788_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75833.1"
                     /translation="MHDSKGLAAPIDPPRGSKSLQVGLLALGCAALALAVPFLGNPWL
                     YALAALLLACFLLPVWNDLQLGRLDPFETIHVMGLRYFAFFGLGAVWTFQNPGDVAYD
                     KYIPPYLLPATFYCVIGYFALLFAYYAPPFRRDAAPAVEERPNGIVFTLIPGAIGLLG
                     SMAAALWNWAAWAGIALSGAVSALGQLTPLYHFAWALCWLLVFSRSTSRPQRLALLAF
                     FLPASVLIVASTLTDKSLAMTIAGTPLAALWYARGKLPWKTLCALLLVLIFVIFPFFN
                     TFRLFDPRIPLSNRIEMTTRVIRTWDLEAYLAQSATAVQRRLALINSVAVVVRDTPRW
                     VPYAEGSTLFLPAIAYFIPRAIWPDKPRFTLGREFGETFRVIHILDEKSSIAATVPGE
                     LYWNFDFPGIVVGMAVWGAAMRFFYVRYGQAHGQDPVRRAIHILLLIQFVHFGGGLAA
                     QSVLLLRTLILL"
     gene            complement(1540..2799)
                     /locus_tag="VD788_05890"
     CDS             complement(1540..2799)
                     /locus_tag="VD788_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75834.1"
                     /translation="MSRARVTPARVVAVLVVAVFLVRAINGLVGGWADSQGRRLAAAG
                     RLADALPLLDRGAIGEDRAATRWLSGEARLGLYQVALYEPETEEGLDELLARAFTDHT
                     EAISLSPASGWYWASLADVYHQHERRQRSPDGLPLEILDQNRWTFVGRPGRVAIGLLR
                     MAIDREPTQYLFHDQLAFALLDYRLIDAAVEVVGESARVQPDFGRHEYRRLSPIPEVV
                     IDAFADASREALGRVPLMRQFGHLLSLGKLELRRGRAGQAEQDLRAALELPGTSLNHA
                     DARYRLGLALAAQSRHEEAVEVLREAEVEPIFEPMALAARASIAEQRGELDQALALLR
                     RARRLAPMELGYAMAYARLAIAVDRPDWAEEALDWAVTAHPDDPRPLRALARLLRELG
                     KRHEASEVERRLLRIGGGGEAGIDSGS"
     gene            complement(2796..4322)
                     /locus_tag="VD788_05895"
     CDS             complement(2796..4322)
                     /locus_tag="VD788_05895"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXV75835.1"
                     /translation="MNGLREEVDGFSLTEILEPEDYLLLAAVVGLPWAFGGVQIWAFR
                     LAALLIVSAAAVVVFRRGAAGLALTRRAGWLLPAPLLGLWGLVQLTPMPPAVVGLLSP
                     RADAIYRMSFPGYPAESAPPTQRAIEDQALARVPEIAGLDAPADGPLAPEMAVGGRWS
                     GWRGLALVPHAGIERVCWFSALLVAFVVARARCRDPEVADAYRKVLFVLFFSLAVFGL
                     VYAATSNGKLYWVRTTLDASRPFGPYVNPTNFAGVMELAVPWLAGYTWLSWSTRSRTT
                     PLRETRLPFLLAATAVCALAALATASKAAALLSLGALAAVALVAAVGWRRRLVVVAAV
                     VVATSAAGLLLRFAPLGARVRDFVEATGGNVSQVDRVVAWRAGLDLLREFPVTGVGFG
                     SFGDVFPAYLPAGEFLRWSHLHNDYLELALEGGVVAVALALWLAVAYWRRVLAGAGRQ
                     TDGGRSNLAHVGLLAGVAALTIHALFDFNHQIPANGLLFTVLAAIAVTMTESRARSGR
                     "
     gene            complement(4319..>4598)
                     /locus_tag="VD788_05900"
     CDS             complement(4319..>4598)
                     /locus_tag="VD788_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75836.1"
                     /translation="VVPLDAGWDDVGSWDAAARLRANGGPAERDVIAIDSSGSAVFAA
                     GRVVALVDVPNVVVVDTEDALLIVSRGQTEKVKRVVDELRRSGRSDLL"
BASE COUNT          811 a         1639 c         1603 g          545 t
ORIGIN      
        1 agcaggatca gggttctcag cagcaggacc gactgcgcgg ccagaccgcc cccgaagtgg
       61 acgaactgga tcaacagcag gatgtggatc gcgcggcgga ccgggtcctg gccgtgcgcc
      121 tgaccgtagc gcacgtagaa gaaacgcatc gcggcacccc agaccgccat accgacgacg
      181 attcccggga agtcgaagtt ccagtacagc tcgccgggca ccgtcgcggc gatgctcgac
      241 ttctcgtcga ggatgtggat cacgcggaag gtctcaccga actcccggcc cagcgtgaac
      301 cgcggcttgt cgggccagat cgcccgcggg atgaagtagg cgatcgccgg caggaacagc
      361 gtactgcctt cggcgtaggg cacccagcgc ggtgtgtcgc ggacgacgac ggcgaccgag
      421 ttgatcaagg ccaatcggcg ctggaccgcg gtcgcggatt gggccaggta ggcctcgagg
      481 tcccacgtcc ggatgacgcg agtggtcatc tcgatccgat tcgacaacgg gatgcgcggg
      541 tcgaacaggc ggaacgtgtt gaagaacggg aagatcacga agatcagcac cagcaacaac
      601 gcgcacagcg tcttccacgg gagcttgccc cgcgcgtacc acagggcggc gagcggcgtt
      661 ccggcgatcg tcatcgcgag cgacttgtcg gtcagcgtgc tggcgacgat caacaccgag
      721 gccggcagga agaacgcgag caacgcgagg cgctgcggcc gcgaggtcga tcgcgagaat
      781 accagcagcc agcacaacgc ccaggcgaag tgatagagcg gggtcagctg gccgagcgcg
      841 ctcaccgccc ccgacaacgc gatcccggcc cacgcggccc agttccacag cgcggccgcc
      901 atcgaaccga ggagcccgat cgctccggga atcagcgtga acacgatgcc gttcggtcgt
      961 tcctcgacgg ccggagcggc gtcccggcgg aacggcggag cgtagtaggc gaacagcagg
     1021 gcgaagtagc cgatcacaca gtagaaggtc gcgggcagga ggtagggtgg gatgtacttg
     1081 tcgtaggcga cgtcgcccgg gttctggaag gtccacaccg cacccaagcc gaagaaggcg
     1141 aagtagcgca gacccatgac gtgaatcgtc tcgaacgggt cgagccggcc gagctgcagg
     1201 tcgttccaga ccgggaggag gaaacaggcc aatagcagcg cagccagcgc gtagagccag
     1261 gggttgccga gaaacggcac ggcgagcgcg agcgccgcgc agcccagcgc cagcaaaccg
     1321 acctggagcg atttcgagcc gcgcggtggg tcgatcggtg ccgcgagccc cttcgagtcg
     1381 tgcatgtccg tgcgcacgtc cggaccgccg ttcgacccgt accagagctg tcgtcgtctc
     1441 tcgagggccg ggggaggcct ggacgagaaa cttatacggc catggtcgcg ccaagtcagc
     1501 gccgaatcga gaccggtcgg gacggcgaag cagaatacct caagaaccgc tgtcgatccc
     1561 ggcttcccct ccccccccga ttcgcagcag gcggcgctcg acctcgctcg cctcgtgccg
     1621 cttgcccagc tcgcgcagca gtcgcgcgag cgcccggagc ggacgagggt cgtcggggtg
     1681 ggcggtcacc gcccagtcca gcgcctcctc ggcccagtcc gggcggtcga cggcgatcgc
     1741 caggcgcgcg taggccatcg cgtaccccag ttccatcggc gcgagccgcc gggcgcggcg
     1801 caacagcgcc agcgcctggt cgagctcgcc tcgctgctcg gcgatcgatg cgcgggccgc
     1861 gagcgccatc ggctcgaaga tcggctcgac ctccgcttcg cgcaggacct cgaccgcttc
     1921 ctcgtgccgc gactgggccg cgagcgcgag gcccaggcgg tagcgcgcgt cggcgtggtt
     1981 caggctcgtc ccgggcagct cgagcgccgc gcgcaggtcc tgctcggcct gacccgctcg
     2041 tccgcgccgc aactccagct tgcccaacga caacaggtgt ccgaactgtc gcatcagcgg
     2101 aacccgcccc aacgcctcgc gcgacgcgtc ggcgaaggcg tcgatcacga cttccggaat
     2161 cggcgacagc cggcggtact cgtgccggcc gaagtccggt tgcacccgcg ccgactcgcc
     2221 cacgacctcc accgccgcgt cgatcagccg gtagtcgagc agggcgaacg cgagctgatc
     2281 gtggaacagg tactgggtcg gctcgcggtc gatcgccatg cgcaacagtc cgatcgcgac
     2341 ccgaccgggc cggccgacga aggtccagcg gttctggtcg aggatctcga gcggaaggcc
     2401 gtccgggctc cgctggcgcc gctcgtgctg gtggtacacg tcggccagcg aggcccagta
     2461 ccagcccgaa gccgggctga ggctgatcgc ttccgtgtga tcggtgaagg cgcgggccaa
     2521 cagctcgtcg agcccttcct cggtctccgg ttcgtacagc gcgacctggt acagccccaa
     2581 ccgcgcctcg cccgacagcc agcgcgtcgc ggcgcgatcc tcgccgatcg ctccccgatc
     2641 gagcagcggg agtgcgtcgg cgagtcgacc ggccgccgcc agccggcgcc cctggctgtc
     2701 ggcccagccg ccgacgagac cgttgatcgc gcggaccagg aacacggcga cgaccagcac
     2761 cgcgacgacc cgcgcgggtg tcacccgcgc gcgactcatc tcccgctccg cgcgcggctc
     2821 tccgtcatcg tcaccgcgat ggcggcgagg acggtgaaca gcaggccgtt ggccgggatc
     2881 tggtggttga agtcgaacag cgcgtggatc gtgagcgccg ctacgccggc gaggagcccg
     2941 acgtgggcca ggttcgaacg gccgccgtcg gtctggcggc cggctcccgc cagcacgcgg
     3001 cgccagtacg cgaccgccag ccacagcgcg agcgcgacgg cgacgacccc gccctcgagc
     3061 gccagctcga ggtaatcgtt gtgcaggtgc gaccagcgga ggaactcccc cgcggggagg
     3121 taggccggga acacgtcacc gaacgagccg aagccgacgc cggtgacagg gaactcccgc
     3181 agcaggtcga gacccgcccg ccaggcgacc acgcgatcga cctgggagac gttgcctccc
     3241 gtcgcctcga cgaagtcccg caccctggcg ccgagcggcg cgaagcgcag cagcagaccg
     3301 gcggccgagg tggcgacgac gaccgcggcg acgacgacca ggcgccgacg ccacccgacg
     3361 gcggcgacca gcgccacggc ggccagcgcg ccgagcgaca ggagtgccgc cgccttgctc
     3421 gccgtcgcga gcgccgccag cgcgcagacg gcggtcgcgg cgagcaggaa cggcagccgg
     3481 gtctcgcgca gcggcgtggt gcgcgagcgg gtcgaccacg acagccaggt gtaacccgcg
     3541 agccatggga ccgcgagctc catcacgccg gcgaagttcg tcggattgac gtacgggccg
     3601 aacggacggc tcgcgtcgag cgtcgtccgc acccagtaca gcttgccgtt cgaggtcgcc
     3661 gcgtagacca ggccgaagac ggcgagcgag aaaaacagga cgaacagtac cttgcggtag
     3721 gcgtcggcca cctcggggtc ccggcagcgc gcgcgcgcga cgacgaacgc cacgagcagc
     3781 gccgagaacc aacagacccg ctcgatgccc gcatggggca cgagcgccag gcctctccag
     3841 ccggaccagc gcccgccgac tgccatttcg ggcgcgagcg gtccgtccgc aggggcgtcg
     3901 agaccggcga tctcgggcac ccgcgcgagt gcctgatcct cgatcgcgcg ctgggtcggc
     3961 ggcgcggact cggcgggata gccggggaag ctcatccggt agatcgcgtc ggcgcgcggg
     4021 ctgagcagcc cgacgactgc aggcggcatc ggggtgagct gaaccagccc ccacagaccg
     4081 agcaacggcg ccggtagcag ccagcccgcg cgccgggtca aggcaagacc ggccgcccct
     4141 cggcgaaaga cgacgaccgc cgccgcgctc acgatcagca gcgcggccag ccggaaggcc
     4201 cagatctgga caccgccgaa ggcccacggc aacccgacga ccgccgccag caacaggtag
     4261 tcctcgggtt cgagaatctc cgtcagggag aacccgtcca cttcctcccg caaaccgttc
     4321 ataacagatc gctgcgcccg gaacgacgca gctcgtcgac gacgcgcttg accttttcgg
     4381 tctgaccgcg cgagacgatg agcaacgcgt cttcggtgtc gacgaccacc acgttcggga
     4441 cgtcgaccaa cgcgacgacg cgtcccgcgg cgaagaccgc gctgcccgag ctgtcgatcg
     4501 cgatcacgtc ccgctcggcc ggaccgccgt tcgcgcgcag ccgtgccgcg gcgtcccacg
     4561 aaccgacgtc gtcccaaccg gcgtcgagcg gtaccacc
//
LOCUS       DASPGN010000208         7925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_504992, whole genome shotgun sequence.
ACCESSION   DASPGN010000208 DASPGN010000000
VERSION     DASPGN010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504992"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /locus_tag="VD788_05905"
     CDS             complement(<1..1727)
                     /locus_tag="VD788_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXV75837.1"
                     /translation="MPIKPPVAKRTEHVEVRHGGRVADPYAWLRDDSREDPEVLAYLE
                     AENAYTEAMMRPVESLRRRLYDEMVARIKEDDSTVPVRKDDYFYYSRTERGKQYRIHC
                     RRRGGLDAAEQVVLDENQLAEGHDYLRVGAFAVSPDHRLLAFSTDFDGSERFTLRVKD
                     LDSGRLLPDSIDETYYSVEWAGDSRTLFYTRIDRTHRPFRLFRHRLGQPAADDRLVYE
                     ESDPAFYLGLRKTKDDRFLVLSLESAVTSEERFLDAGDADGLFRLIEPRRNRHEYAAE
                     HWNGRFLILTNDDAVNFKLVETPVETPSREHWRTMIDHRDEVKLDAIEVFRDRIAVLK
                     RERGLREIDVIDRLSAETRRIEFSEPAYDVYFSGNAEFDTDTLRFEYESMTTPRSTYD
                     YEMPTGERTLLKRIEVLGGFDPERYVAERLFADSVGGKQVPISLVYRKGALDDGPAPL
                     LLYGYGAYGATIDPGFSSARVSLLDRGMVYAIANVRGGGALGRPWYEDGKLLAKRNTF
                     DDFIAAAEQLIGRGYTASDRLAIAGGSAGGLLVGAALNMRPELFRAAVAKVPFVDVVN
                     TMLDPTIPLT"
     gene            complement(1841..4786)
                     /locus_tag="VD788_05910"
     CDS             complement(1841..4786)
                     /locus_tag="VD788_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXV75838.1"
                     /translation="MFAGRSSEMNHDLEALFRAVRDGCSPARWSRGVELSRSGGVFLE
                     SRNDDELVFRIAGSRPAGRTLRLFLDDHEWDCGCDSPEPGCEHVAAAVIVWKRAAEAG
                     VQLQSAARVAGRVSYRFERGPGGLALGRVLVTDDGESILERTLAALAQRDPMLHARVV
                     SQADLAVELAMGTYRRGALPPRIVRKLFARVAQCQDVRLAGLPVEVSAEPVRPLVRVE
                     DQHDGFRLVLIDDAAVSETFANGIVLRDGVLRPLDETGLDGRERNELPRGIRFGPDRV
                     AELVTEVIPSLGRRVRLEIVTTRLPQGVKEAPRIRLDAERDGERLSVLATLVYGDPPR
                     ARVDAGRLVHLAGAVPIRDERAERALTRRLQRELGLVPGLRADFVGEQAVAFNRRLEH
                     REGVDAAAAALRFFELRPPLRPSFRLRGDDFELSFDCDAVAGTRAGRAETERVIRAWC
                     DGASLVPLTDGGYAPLPIEWLARNGERVADLLAARQPNGALPPACLPDLAALCAELDQ
                     PEPPGFTRLGRLVHGFDGLPAATLPADLTASLRPYQRAGVDWLVFLRDAGLGALLADD
                     MGLGKTLQALCAIRGRTLVVSPTSVLHNWADEITRFRPALTVSIYHGPTRALDPAADV
                     TLTTYALLRADADRLCAERWSTVVLDETQAIKNPESQVARAAYRLDADFRVALSGTPV
                     ENRLDELWSQFHYTNSGLLGGRSDFQRRYAAPIDAGDATVAGRLRDRIRPFVLRRLKQ
                     QVAPELPPRTDLVLYCELDPQERELYDTIRAATLREVVDKLARGAGVLAALEALLRLR
                     QAACHSALIPGQQASDSSKLRTLMEALDNVVAEGHKAIVFSQWTSLLDLTEPALRRAG
                     IDHLRLDGSTRDRGSVVRGFQDLAGPPLLLVSLKAGGTGLNLTAADHVFLLDPWWNPA
                     VEDQAADRAHRIGQLVPVFVYRLVAEQTVEERILDLQRRKRAVADAALGQADAAAAIT
                     REELMELLG"
     gene            complement(4759..6174)
                     /locus_tag="VD788_05915"
     CDS             complement(4759..6174)
                     /locus_tag="VD788_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HXV75839.1"
                     /translation="MRSTGASSRHGRSFTGSDSFWLRMDAPTNLMMISAVLLFEQPLD
                     HARLRRVVRERLLPVDRFRDRVVRAADGTARWERHPALDLDQHLVVLRLPEPADEAAL
                     CAAVGRLMSEPLDPSRPLWQFHLIERYGTGSALIGRFHHCLGDGLGMMIALLAMSDLD
                     PDGGATTGDRANPFLALLRNPGEGTDRVRRGLEQIMPEGTRLLLRPGEALRRLGPLKR
                     TTAASLALARLVFRRPDRRTLFNAELGVAKRAAWSRRIPVERVKGVRDAIGGTVNDIL
                     LTAMAGGIRRYVVARDGEAGCMDFRATVPVNLRPIEQLAAMGNEFGLVYLRLPIAIAD
                     PIERLAELRRRMEPLKRSAEALVVLRLIATIGRLPEFAQRQAVRLFASKATSVMTNVP
                     GPIRTLYLAGRPVADLIFWVPRSGGLSLGVSIMSYAGNVRLGVATDAGLVPDPERIVE
                     GFDAEFAAMMQLRCSPDGRPR"
     gene            complement(6262..7182)
                     /locus_tag="VD788_05920"
     CDS             complement(6262..7182)
                     /locus_tag="VD788_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75840.1"
                     /translation="MPSHRTSAIPILLAVTAAAWAPAAGGTFEDSVRSRWRGAWVVLG
                     TEVYSTCDGAYHANRVSRSLVAHRGGMRFLPGELGEVQRVQLKKRKVELMIALDVPLR
                     VAAQDGPFTLYDHRPCAISLEIEVPRDATAAQDVQAVDRIAAGVARRFDTREDAMADA
                     GWNARQPEPLPPDHDLTVARHARWKAEQTNASVDGKREAAVDQARLAGTTIDDDPSYL
                     AGLAAGVEQMRGWQERDCARLMTREFDALERKAPEPQRSDHRWLRGFADGQRLVYYIE
                     LIDRLESCYLPLPPLPVETAAEASASLERH"
     gene            complement(7185..>7925)
                     /locus_tag="VD788_05925"
     CDS             complement(7185..>7925)
                     /locus_tag="VD788_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXV75841.1"
                     /translation="LIVAAEDGYRRGVKIRLVNEGCRVGETSSFKRALSKARRGEVDL
                     LLVDLDADRAEAFQLLREVRDHGELRHVPLAFVSRRSDRILKLRALREGVDDYLNKDD
                     DLETTVARLQNTLAREAARGEARTIRARRGIRGDLDSFGFADIVQMLSIGMKTARVTL
                     TREGVKATVWFESGAPRHAETDGARGESVFLQLVGWHQGSFVIEHGVRSDERTIENDA
                     MFLLMEAMRLFDESTEDPTRQATSSTAS"
BASE COUNT         1235 a         2837 c         2701 g         1152 t
ORIGIN      
        1 gtcagcggaa tcgtcggatc gagcatcgtg ttcaccacgt cgacgaacgg caccttggcc
       61 accgccgcgc ggaacagctc cgggcgcatg ttgagcgccg cgccgacgag caggccgccg
      121 gcgctgcccc cggcgatcgc caaccggtcg ctggcggtat acccgcgacc gatcagctgc
      181 tcggccgccg caatgaagtc gtcgaacgtg ttgcgcttgg ccagcagctt gccgtcctcg
      241 taccacgggc gtcccagcgc gccgccgcca cgcacgttgg caatggcgta gaccatccct
      301 cgatcgagca ggctgacgcg cgcggaggag aaccccggat cgatcgtcgc gccgtaggcg
      361 ccgtagccgt agagcagcaa cggcgccggg ccgtcatcga gcgctccctt gcggtagacc
      421 agcgagatcg gcacctgctt gccgccgacg ctgtcggcga acaggcgctc ggcgacgtag
      481 cgctccgggt cgaagccacc gagcacctcg attcgcttga gcagcgtgcg ctcgccggtc
      541 ggcatctcgt agtcgtaggt cgacctgggg gtcgtcatcg attcgtattc gaagcgcaac
      601 gtgtcggtgt cgaactcggc attcccgctg aagtagacgt cgtacgccgg ctccgagaac
      661 tcgatccggc gggtctcggc cgagagccgg tcgatcacgt cgatctcgcg caacccccgt
      721 tcgcgtttga gcacggcgat gcgatcgcga aagacctcga tcgcatcgag cttgacctcg
      781 tcgcggtggt cgatcatcgt ccgccagtgc tcgcgtgacg gggtctcgac cggcgtctcg
      841 accagcttga agttgaccgc gtcgtcgttg gtcaggatga ggaatcgtcc gttccagtgc
      901 tcggccgcgt actcgtggcg gttgcgtcgc ggctcgatca gacggaacag gccgtcggca
      961 tcgcccgcat cgaggaaacg ctcctccgac gtgacggcac tctcgagcga caaaacgagg
     1021 aaccgatcgt ctttcgtctt gcgcaggccg aggtagaacg ccggatccga ctcctcgtag
     1081 accaaacgat cgtccgccgc cggctggccc aaccggtgac gaaacagccg aaacggccgg
     1141 tgggtccgat cgatgcgggt gtagaacagc gtccggctgt cgccggccca ctcgaccgag
     1201 tagtacgtct cgtcgatcga gtcgggcagc agccggccgc tatcgaggtc cttgacgcgc
     1261 agggtgaacc gctcgctgcc gtcgaagtcg gtcgagaacg cgagcaaccg atggtcgggg
     1321 ctgaccgcga acgcaccgac gcgcaggtag tcgtgccctt cggccagctg gttttcgtcg
     1381 agcacgacct gttcggcggc gtcgagccct ccccggcggc ggcagtgtat ccgatactgc
     1441 ttgccgcgct ccgtccgcga gtagtagaag tagtcgtcct tgcgcaccgg caccgtgctg
     1501 tcgtcttcct tgatgcgcgc gaccatctcg tcgtagagcc gtcggcggag cgactcgacc
     1561 ggccgcatca tcgcctcggt gtaggcgttc tcggcctcga ggtaggccaa cacctccggg
     1621 tcctcgcgcg aatcgtcgcg cagccaagca taggggtcgg cgactcgccc gccgtgacgc
     1681 acctcgacgt gttcggtgcg ttttgcgaca ggaggtttga tcggcatcgt ggcgctctcc
     1741 gctcggctcg ggtcgcgatc cgagcatccg caggcgacga tcgatcccga cagtacgatg
     1801 tacgcgagca tgcccgcgct tccaacttgt gtcgtctggg tcaaccgagc aactccatca
     1861 gttcctcgcg ggtgatcgcg gccgcggcgt cggcctgacc gagcgccgcg tcggcgaccg
     1921 cccgcttgcg ccgctggaga tcgaggatcc gctcctcgac cgtctgctcg gcgaccagcc
     1981 ggtagacgaa cacgggcaca agctgtccga tccgatgggc ccggtcggcc gcctggtcct
     2041 cgacggcggg gttccaccac ggatcgagca ggaatacgtg atccgccgcc gtgagattca
     2101 aaccggtccc gccggccttg agcgacacca acagcaacgg gggtccggcc aggtcctgga
     2161 acccgcgaac caccgagccg cgatcgcgcg tcgagccgtc gagccgcagg tggtcgatac
     2221 ccgcgcggcg caacgccggt tcggtcaggt cgagcagcga ggtccactgc gagaagacga
     2281 tcgccttgtg gccttcggca acgacgttgt cgagcgcctc catcagcgtg cgcagcttgg
     2341 acgagtcact cgcctgctga ccggggatca acgcgctgtg gcacgcggcc tgcctcaggc
     2401 gcagcaacgc ctcgagcgcg gccagcacgc ccgctccgcg cgcaagcttg tcgacgacct
     2461 cgcgcagcgt cgcggcgcgg atcgtgtcgt acagctcccg ctcctgcgga tcgagctcgc
     2521 agtacaggac gagatcggtg cgcggtggga gctccggcgc gacctgctgc ttgagccgac
     2581 ggaggacgaa cgggcggatg cggtcgcgca ggcgtcccgc gaccgtcgcg tccccggcgt
     2641 cgatcggcgc ggcgtatcgt cgctggaaat cgctgcgccc gccgagcagc ccgctgttgg
     2701 tgtagtggaa ctgactccac agctcgtcga gccgattctc gaccggtgtg ccgctcagtg
     2761 cgacgcggaa gtcggcgtcg agccggtagg ccgcgcgcgc gacctggctt tccgggttct
     2821 tgatcgcctg ggtctcgtcg agcacgacgg tcgaccagcg ctcggcacac agccggtccg
     2881 cgtcggcacg cagcaatgcg taggtcgtca gcgtgacgtc ggccgccggg tcgagcgcgc
     2941 gggtcggtcc gtggtagatc gacacggtca acgccggccg aaaccgcgtg atctcgtcgg
     3001 cccagttgtg cagcacgctg gtcggcgaga cgacgagcgt gcggccgcgg atcgcgcaca
     3061 gcgcctgcag cgtcttgccg aggcccatgt cgtcggcgag caacgcgccg agcccggcgt
     3121 cgcgcaggaa gaccagccag tcgacacccg cacgctggta cggacgcagc gacgcggtca
     3181 ggtcggccgg cagcgtcgcg gccggtaggc cgtcgaaccc gtggacgagc cggcccagac
     3241 gcgtaaagcc cggtggctcc ggttgatcga gctccgcgca cagcgcggcc aggtccggga
     3301 gacaggccgg cggcagcgcg ccgttgggct gacgggcggc gagcaggtcg gcgacgcgtt
     3361 cgccgtttcg cgccagccac tcgatcggaa gcggcgcgta gccgccatcg gtcaacggca
     3421 cgagcgaggc cccgtcacac caggcccgga tcacccgctc ggtctcggcg cggcccgcgc
     3481 gcgtgccggc caccgcgtcg cagtcgaacg agagctcgaa gtcgtcgccg cgcaggcgga
     3541 agctcggccg cagcggcggt cgcagctcga agaaccgcag cgcggccgca gcggcgtcca
     3601 ctccctcgcg gtgctcgagc cgccggttga aggcgacggc ctgctcgccg acgaagtcgg
     3661 cgcgcaagcc gggcacgagc ccaagctcac gctgcagccg gcgggtcagg gcgcgctcgg
     3721 cgcgctcgtc gcggatcggc accgcgcccg ccaggtgcac gagccgtccc gcgtcgaccc
     3781 gcgcgcgcgg cggatcgccg tagaccagcg tggccagcac cgacaaccgc tccccgtccc
     3841 gctcggcgtc gagacggatg cgcggcgcct ccttcacgcc ctgcggcaga cgagtggtga
     3901 cgatctcgag ccggacgcgt cgcccgagcg aagggatcac ctcggtgacc aactcggcaa
     3961 cacggtccgg accgaaacgg atgccgcgcg gcaactcgtt gcgctctcgg ccgtcgaggc
     4021 ccgtctcgtc cagcggtcgc agcacgccgt cgcgcaggac gatgccgttg gcgaaggtct
     4081 cgctgaccgc cgcatcgtcg atcagcacca gccggaagcc gtcgtgctgg tcctcgacgc
     4141 gaaccagcgg ccgcaccggc tcggccgaga cctcgaccgg cagaccggcg agccgcacgt
     4201 cctggcactg cgccacccgg gcgaacagct tgcgcacgat ccgcggaggc agcgcccctc
     4261 ggcggtaggt acccatcgcc agctcgaccg ccagatccgc ctggctcacg acacgggcat
     4321 ggagcatcgg atcccgttgc gcaagggcgg ccagcgtccg ctcgagaatc gactcgccgt
     4381 cgtcggtgac gagcacgcgg ccgagcgcca agccccccgg cccgcgctcg aaacgataag
     4441 acacgcggcc ggcgacccgt gccgcggact gcaactgcac gccggcctcg gcggcacgct
     4501 tccagacgat gaccgcggcc gcgacgtgct cgcaccccgg ctccgggctg tcgcagccgc
     4561 agtcccactc gtggtcgtcg aggaacagcc gcagcgtgcg gccggcggga cgcgaccccg
     4621 cgatgcggaa gaccagctcg tcgtcgttgc ggctctcgag gaagaccccg cccgaacgac
     4681 tcagctcgac cccgcgcgac cagcgtgccg gcgagcagcc gtcgcgcacg gcgcgaaaca
     4741 acgcttcgag gtcgtgattc atctcggacg accgtccggc gaacatcgga gctgcatcat
     4801 cgcggcgaac tcggcgtcga acccttcgac gatccgctcg gggtcgggca ccagacccgc
     4861 atcggtcgcg acacccaagc gaacgttgcc ggcgtagctc atgatgctga cccccaggct
     4921 gagacccccc gagcgcggca cccagaagat caggtcggcg accggccggc cggcgaggta
     4981 caacgtgcgg atcggccctg ggacgttggt catcaccgac gtcgccttcg aggcgaacag
     5041 ccgcaccgct tggcgctggg cgaactccgg cagccgaccg atcgtcgcga tcagccgcag
     5101 gacgaccagc gcctcggccg atcgcttcag cggctccatc cggcgccgca gttcggccaa
     5161 ccgctcgatc gggtcggcga tcgcgatcgg caggcgcaga tagaccagac cgaactcgtt
     5221 tcccatcgcg gccagttgct cgatcggacg caggttgacc ggcaccgtag cgcggaaatc
     5281 catgcagccg gcctcgccgt cgcgcgcgac gacgtagcgg cggatcccgc cggccatcgc
     5341 cgtcaacaag atgtcgttca ccgtgccgcc gatcgcgtca cgcacgccct tgactcgctc
     5401 gaccgggatc ctgcgcgacc acgcggcacg cttggcgacg ccgagctcgg cgttgaacag
     5461 cgtccggcgg tccggccggc gaaagaccag tcgggccagc gcgagcgacg ccgcggtcgt
     5521 ccgcttcagt ggcccgagtc gccgcagcgc ttcgccgggt ctgagcagca atcgcgtgcc
     5581 ctcgggcatg atctgctcga gcccgcgacg gacacgatct gtcccctctc ccgggttccg
     5641 caacagcgcg agaaacgggt tggcccggtc gccggtggtc gcgccgccgt cgggatcgag
     5701 atcgctcatc gccagcagcg cgatcatcat ccccaacccg tcgcccaggc aatggtggaa
     5761 ccggccgatc aacgccgagc cggtcccgta acgctcgatc aagtggaact gccacagcgg
     5821 gcggctcgga tccaacggct cgctcatcag cctgccgacc gcggcgcaga gcgcggcctc
     5881 gtccgccggt tcgggcaagc gcaggacgac cagatgctgg tcgaggtcga gcgccgggtg
     5941 acgctcccag cgcgccgtcc cgtccgccgc gcgcaccacg cgatcccgga agcgatcgac
     6001 cggcagcaag cgctcgcgga cgactcggcg cagccgcgcg tgatcgagcg gttgctcgaa
     6061 gagcaacacg gcgctgatca tcatcaggtt ggtcggggcg tccatcctca gccagaagct
     6121 gtcgctgccg gtgaaagacc ggccgtggcg cgacgaggcc ccggtcgatc tcaacgcggc
     6181 acgtggagcg aagccgggac gtcggcaccg gcgacgaacc tgcggatcat acggctagga
     6241 taacccagcc agcggtggcg gtcagtgtcg ttcgagcgac gcggaagcct cggctgccgt
     6301 ctcgaccggc agcggcggca gggggagata gcacgactcc agccggtcga tcagctcgat
     6361 gtagtagacg agacgctggc cgtcggcgaa accacggagc caccggtggt cgctccgctg
     6421 cggctcgggc gccttgcgct cgagcgcgtc gaactcgcgc gtcatcagcc tggcgcagtc
     6481 gcgctcctgc cagccacgca tctgctcgac gccggcggcc agaccggcca aatagctcgg
     6541 gtcgtcgtcg atcgtcgtcc cggccagccg ggcttgatcg accgccgcct cgcgcttgcc
     6601 gtcgacggaa gcattggtct gttccgcctt ccagcgcgcg tgccgcgcca cggtcaggtc
     6661 gtgatcgggc ggcagaggct ccggttggcg ggcattccat ccggcgtcgg ccatcgcatc
     6721 ctcgcgcgtg tcgaatcgtc gcgcgacccc ggcggcgatg cgatcgaccg cctgcacgtc
     6781 ctgcgcggcg gtcgcgtcgc gcggcacctc gatctcgagc gaaatcgcgc atgggcggtg
     6841 gtcgtagagc gtgaacgggc cgtcctgagc ggcgacgcgc aacggcacgt cgagcgcgat
     6901 catcagctcg accttgcgct tcttcagctg cacgcgctgc acttcgccga gctcgcccgg
     6961 aaggaagcgc atcccgccgc ggtgtgcgac cagactccgg ctgacgcgat tggcgtggta
     7021 agcgccgtcg caggtcgagt agacctcggt acccagcacg acccacgcgc cgcgccatcg
     7081 cgagcggacg gaatcctcga acgtaccgcc agccgccgga gcccacgcgg cggcggtgac
     7141 ggccagcagg atcgggatcg cgctcgtacg gtggctcggc atggtcagga ggcggtcgac
     7201 gaggtcgcct gccgcgtcgg gtcctcggtc gactcgtcga agagccgcat cgcttccatc
     7261 agcaggaaca tcgcgtcgtt ctcgatcgtg cgctcgtcgc tccgcacgcc gtgctcgatg
     7321 acgaacgacc cctggtgcca accgacgagc tgcaggaaga ccgattcgcc gcgtgcgccg
     7381 tcggtctccg cgtggcgcgg cgcgccgctt tcgaaccaga ccgtcgcctt gacgccctct
     7441 cgcgtcagcg tgacacgcgc cgtcttcatc ccgatcgaca gcatctggac gatgtcggcg
     7501 aagccgaagc tgtccaggtc gccgcggatc ccgcgccggg cccgtatcgt ccgcgcctct
     7561 ccgcgggcgg cctcgcgggc caacgtgttc tgcagccgcg ccaccgtcgt ctcgagatcg
     7621 tcgtccttgt tcaggtagtc gtcgacgcct tcccgcagtg cccgcagttt gaggatgcgg
     7681 tcggaccgtc gcgagacgaa cgcgagcggg acgtgacgca gctcgccgtg atcccgcacc
     7741 tcgcgcagta gctggaatgc ctcggcgcgg tcggcgtcga gatcgacgag cagcagatcg
     7801 acttcgcccc tgcgcgcctt gctcagcgcg cgcttgaagc tgctggtctc gccgacgcga
     7861 catccctcgt tgaccaggcg gatcttgacg ccgcgacggt agccgtcctc ggcggcgacg
     7921 atcag
//
LOCUS       DASPGN010000209         7804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1675979, whole genome shotgun sequence.
ACCESSION   DASPGN010000209 DASPGN010000000
VERSION     DASPGN010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1675979"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="VD788_05930"
     CDS             <1..1173
                     /locus_tag="VD788_05930"
                     /inference="COORDINATES: protein motif:HMM:NF023428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXV75842.1"
                     /translation="ILWGPPGCGKTTLARIIAGRTRADYVPFSAVVSGIKEVRAVMEG
                     AARRFGSGGRRTLLFIDEIHRFNRSQQDAFLPYVEDGTILLIGATTENPSFEINSPLL
                     SRSRVYRLERLSEEEILTLLRRALGDRDRGLGHLDVDFPDELLRVVAEQSQGDARAAL
                     SALELVAEAAAAAGGAVTRDGLVDALQKTTLYYDRAGEEHFNLISALHKSMRNSDPDA
                     TLYWLARMLAAGEDPLYVARRIARFASEDVGLADPRALELAIAAKEAVHFIGLPEGKL
                     ALAEAAVYCALAPKSNALYAAFAEAERAIDEGAHDPVPFAIRNAVTGLMKREGYGAGY
                     RYAHDHDDAITDLDCLPQRLAGRRFYRPTARGFEKTLSERIDAWLDARRRLKDRSE"
     gene            complement(1154..2338)
                     /locus_tag="VD788_05935"
     CDS             complement(1154..2338)
                     /locus_tag="VD788_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75843.1"
                     /translation="MADRSETLGEVAWDALARLVRERMARRPGGHLLETELGRLELEL
                     PLVLRGSDAGPERFAAALVEEIDRLLDDAVQHAASFRPGHAWCHRCERADCEHSRPTG
                     CRHVFVGYGPTGTPRWQDFAQLCLDRQHPNVDRLFDRPPALITLVHDRHELYGGILRV
                     FRRDAYELLGQVTAGFFPIRSRTEEGRGVLALTVQAAASRTRRGDLRIGLNLLGTTPT
                     GEELTRLWDRQEDLPWWTPVRWAQGTLRSLNGGAPLSGGRRMRRDELDRRIDGILRGL
                     ARRIERDRRARSRRTRHAEQRHASGERPTRMALEDARRAAGERFLVDERSGTIVVLGD
                     RGRTHFFAPTGRLVSSVRYRKEAIDRKIKREHWRLAAADEVDALRASLDERPGELTRS
                     GP"
     gene            complement(2398..3534)
                     /locus_tag="VD788_05940"
     CDS             complement(2398..3534)
                     /locus_tag="VD788_05940"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXV75844.1"
                     /translation="MRIGSKGVEYTSVSGAFITLYHLGLLVGLPIYFYFTRPSWALVV
                     VSLVLLFLTELGITAAYHRLYSHRSFNLSRPIEAILLALGTLGLQGSVLQWSHDHRKH
                     HAHVDTDDDPYSINKGFWYAHMLWLYDPPQPIETKWVADLMQNRLVTWQHRYFALLGI
                     GGNLLVFLLIGWLVGDFLGAFVIAWWTRLLVSHHLTWFINSLAHYWGSRTYSREHSAV
                     DNYIIAFLTMGEGYHNYHHTFAADYRNGVRWYHFDPSKWLVWGLERVGLATDLKRYDS
                     VTIKKKLLAEDRRLLLEAVKRRASSTKLELEQRIVHLSEAIRLKLNRIHALAEEVNKL
                     KRGRQDRARVRAALDELKRLKHSLRHDWRSWGRLCGAVLAPHPA"
     gene            complement(3611..4306)
                     /locus_tag="VD788_05945"
     CDS             complement(3611..4306)
                     /locus_tag="VD788_05945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXV75845.1"
                     /translation="MRRLILSSAIGALLFAFAGAGAADPATEDQKLLYALGMALAAKV
                     PAFDPTTEEIAMIQAGITDHLQGNEHKVDMAVYAAKLDAFMQGKMAAVADREKTAGAK
                     FRDEMAAKDGATKTDSGMIYFELQPGNGAQPTASDTVRIHYHGTRVDGTVFDSSVERK
                     EPVEFAVNGVVPCFAEGLTKMKVGGKNKIVCPPELAYGDRGYGGIKPGSTLVFEVELL
                     EVVQPQPAATPTP"
     gene            complement(4392..5864)
                     /locus_tag="VD788_05950"
     CDS             complement(4392..5864)
                     /locus_tag="VD788_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75846.1"
                     /translation="MSFADRPLIVVATVCYLAIVTAVGLWSLGRTRDARDFFIAGQRA
                     GLWVTALATMSAAFSGFVFLGGPGLTYRIGLASLMIVLPLGFTGGLLCRVLGTRLRAL
                     AAEQPIYSVPDAFDARFGSRVVTGLATLAVLSGTVAYLGMQLQALGVLLQAVFGLERG
                     ASAMLLGLAVLVAYSVLGGMVAGVYTDLVQGGLMMLTGIVVFLHAVAAAGGWHRTVAA
                     VAESASFGPQFLEPLGRAAPLTVFGFFFVFGVGVLGQPQMLHKFYMISDPKKLRWFPL
                     VLSGSQAVCVLLWLGVGLAVPALVAGGRLAPLERADEAAVRFLLEFTPELVAGLAVAG
                     ILAAIMSTADSLLNIGAVALVRDLPRVLGLGRPEPLAAGRAAVLIVAGLAALLAFRHG
                     GLIAMLGTMAFGTFGAALAPALAVGFHWRRVGAAAAAASIATGLVVNLGLELWRPAWL
                     ADGVLPAAVALAASFSALFCVAAVIDGAAAGGRRAGSLVK"
     gene            complement(5861..6241)
                     /locus_tag="VD788_05955"
     CDS             complement(5861..6241)
                     /locus_tag="VD788_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75847.1"
                     /translation="MARFSLIALAISSPVLLLALLVGGEAGGFVFAVVAPIVPVGLMA
                     LGRDGGPRRFGVVVWLGLLWLSLEAGTLGILFATGRTGGWLGLPAATVWMLVGLGIVP
                     LLLVPWGFAWLWRDEGPDATRSRR"
     gene            6339..>7804
                     /locus_tag="VD788_05960"
     CDS             6339..>7804
                     /locus_tag="VD788_05960"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXV75848.1"
                     /translation="MQLGFVIDHTRCIGCHACTVACKAENDVPLGSFRTWVKYTERGD
                     FPQVRRSFAVLRCNQCTDPPCVTICPVGALGKRRDGIVDIDPQRCIGCKSCLHGCPYD
                     ALYINDSTGLAEKCHFCAHRSEIGLAPACAVVCPTEAIVPGDFDDPRSHVARLAAEGG
                     LEVRKPEAGTGPNVRYREVDRAGIDPLETNAAGGFLWAEQLPGARLDAARFEAVERPS
                     AARTTYDVEHPPAWGAAITGYLFAKSIAAGAFLAAIPWLGPSAGGSPAAARPALAAVL
                     LALAALAATGALLIADLKRPERFWFLLRYGNPSSWLVRGTWVLIGYGALLVVWLLLLG
                     TGNVPSAPLGPWLVVATSLLAALTACYTAFLFAQARGRVLWMGRGLWLGLLAHAAIAG
                     GAALIPLAAALGSGLETIAPLRRLLVVGLLVHLSLSLAERAFAPRGREEEYRRAVRLV
                     SHGPFARAHWWIGVGAGVVLPIALLVVPSSPAAWTAASV"
BASE COUNT         1210 a         2743 c         2747 g         1104 t
ORIGIN      
        1 atcctgtggg gcccgcccgg ctgcggcaag acgacactgg cccggatcat cgccggccgc
       61 acgcgggccg attacgtgcc gttctccgcg gtcgtctcgg gcatcaagga agttcgggcg
      121 gtgatggaag gcgcggcgcg acgcttcggc agcgggggcc ggcgcacgct gctgttcatc
      181 gacgagatcc accgtttcaa tcggtcccag caggatgcgt tcctgccgta cgtcgaggac
      241 ggcacgatcc tgctgatcgg cgcgacgacc gagaacccgt cgttcgagat caactccccg
      301 ttgttgtcgc gctcccgcgt ctaccggctc gaacggttga gcgaggagga gatcctgacg
      361 ctgctgcgcc gcgcgctcgg cgatcgcgac cgcgggctgg gccacctcga cgtcgacttc
      421 cccgacgagc tgctgcgagt cgtggcggag caaagccagg gcgacgcgcg cgccgcgttg
      481 agcgcgctcg agctggtggc ggaggccgcg gccgccgcgg gcggcgcggt cacgcgtgac
      541 ggcctcgtcg acgcgttgca gaagacgacg ctgtactacg accgtgccgg tgaggagcat
      601 ttcaacctga tcagcgcgct gcacaaatcg atgcgcaact ccgacccgga cgcgaccctc
      661 tactggttgg cgcgcatgct cgccgcgggc gaggacccgc tctacgttgc gcgccgcatc
      721 gctcgcttcg ccagcgagga cgtggggctc gccgacccgc gcgcgttgga gctggcgatc
      781 gcggccaagg aagcggtgca cttcatcggg ctgccggagg gcaagctggc gctggccgag
      841 gccgcggtct actgtgcgct ggcgcccaag tcgaacgcgc tctacgccgc gttcgcggag
      901 gccgagcgcg cgatcgacga gggcgcgcac gacccggtgc cgttcgcgat ccgcaacgcc
      961 gttaccgggc tgatgaaacg cgaaggatac ggcgcgggct accgttacgc ccacgaccac
     1021 gacgatgcga tcaccgacct cgactgcctg ccgcagcggc tggccggccg gcggttctac
     1081 cgcccgacgg cgcgcggatt cgagaaaacg ctgagcgagc ggatcgacgc ctggctcgac
     1141 gcgcgccggc ggctcaagga ccggagcgag tgagctcgcc cggccgttcg tcgagactcg
     1201 cgcgcagcgc gtcgacctcg tcggcggccg ccaggcgcca gtgctcgcgc ttgattttcc
     1261 tgtcgatcgc ttccttgcgg tagcgcacgc tcgacaccag ccggcccgtc ggcgcgaaga
     1321 agtgggtgcg gccgcgatcg ccgagcacga cgatcgtgcc gctgcgctca tcgacgagga
     1381 aacgctcgcc ggcggccctc cgcgcgtcct cgagcgccat ccgcgtcggg cgctcgcccg
     1441 aggcgtggcg ttgctcggcg tggcgcgtcc gtcgcgagcg tgcacgtcgg tcgcgctcga
     1501 tccgccgcgc cagaccccgc aggatcccgt cgattcggcg atccagctcg tcgcggcgca
     1561 tgcggcgtcc gccggacagc ggtgcgccgc cgttgagcga gcggagcgtc ccctgggccc
     1621 agcgcaccgg agtccaccac ggcaggtcct cctgccgatc ccacagccgg gtcagctcct
     1681 cgccggtcgg cgtcgttccg agcagattga gcccgatgcg cagatcgccg cgccgcgtac
     1741 gggacgcggc agcttgcacg gtcaaggcca gcacgccccg cccttcctcc gtccgcgagc
     1801 ggatcgggaa gaagccggcc gtgacctgac ccaacagctc gtatgcgtcg cgccggaaga
     1861 cgcgcaggat gccgccgtac agctcgtgcc ggtcgtggac cagcgtgatc agcgccggcg
     1921 gccggtcgaa caaccggtcg acgttcgggt gctggcggtc gagacagagc tgggcgaaat
     1981 cctgccagcg tggggtaccg gtcgggccgt agccgacgaa gacgtgacgg cagccggtcg
     2041 ggcgcgagtg ctcgcagtcg gcgcgctcgc agcggtgaca ccaggcatgc ccggggcgga
     2101 acgacgcggc gtgctgcacc gcgtcgtcca gcaggcggtc gatttcctcg acgagcgccg
     2161 ccgcgaagcg ctcgggcccc gcgtcggagc cgcgcaggac cagcgggagc tcgagctcga
     2221 gtcggccgag ctcggtctcg agcaggtggc cacccggccg ccgggccatc cgctcgcgga
     2281 cgagacgcgc gagcgcgtcc cacgccacct cgccgagggt ctccgatcga tcggccatgg
     2341 tgccattatg cgccgactag gacgaacgcg cggcggcgag ccggcgaccg ccgcggctca
     2401 ggcgggatgg ggcgcgagca ccgcgccgca caatcggccc cacgagcgcc agtcgtggcg
     2461 caggctgtgc ttcagccgtt tcagctcgtc gagggccgcg cgaacgcggg cgcggtcctg
     2521 ccggccgcgc ttcagcttgt tgacttcctc ggccagcgcg tggattcgat tgagcttcag
     2581 gcgaatcgcc tccgacaggt ggacgattcg ttgctccagc tcgagcttgg tgctcgacgc
     2641 gcggcgtttg accgcttcga gcagcagcct gcgatcctcg gcgagcagct tcttcttgat
     2701 cgtgacgctg tcgtagcgct tgaggtcggt cgccaggccg acccgctcca gcccccatac
     2761 cagccacttc gacggatcga agtggtacca gcgcacgccg ttgcggtagt cggcggcgaa
     2821 ggtgtggtgg tagttgtggt acccctcgcc catcgtcagg aacgcgatga tgtagttgtc
     2881 gacggccgag tgctcgcgcg aataggtgcg cgacccccaa tagtgcgcca gcgagttgat
     2941 gaaccaggtc aggtgatggg agacgagcag tcgcgtccac caggcgatga cgaacgcgcc
     3001 gagaaagtcg ccgaccagcc agccgatcag caggaacacg agcagattgc cgccgatgcc
     3061 gagcagcgcg aagtagcgat gctgccaggt caccagacgg ttttgcatca agtcggcgac
     3121 ccacttcgtc tcgatcggct ggggcgggtc gtacagccac agcatgtgcg cgtaccagaa
     3181 gcccttgttg atcgagtacg ggtcgtcgtc ggtgtcgacg tgggcatggt gcttgcgatg
     3241 atcgtgcgac cattgcagga cgctgccctg caggccgagc gtgccgagcg ccagcaggat
     3301 cgcctcgatc ggtcggctga ggttgaagct gcggtgcgag tagagacggt gataggccgc
     3361 ggtgatgccg agctcggtca ggaacagcag cacgagcgaa acgacgacca gcgcccacga
     3421 cgggcgcgtg aagtagaagt agatcggaag accgacgagc aggcccagat ggtagagcgt
     3481 gatgaacgca cccgagaccg atgtgtattc gacgcctttg ctgccgattc tcacgccgca
     3541 tcactccctg ccgtcactcg ccggacaccg tccgggtggg ccgaatataa gctccgtacg
     3601 ccggaaagtc tcacggggtg ggagtcgcgg ccggctgcgg ctggaccacc tcgagcagct
     3661 cgacctcgaa aaccagcgtc gaacccggct tgatcccgcc gtagccgcgg tccccgtagg
     3721 ccagctccgg cgggcagacg atcttgttct tgccaccgac tttcattttc gtcaggccct
     3781 cggcgaagca cggaacgacg ccgttgacgg cgaactcgac cggctctttg cgctcgaccg
     3841 agctgtcgaa gacggtgccg tcgacgcgcg tgccgtggta gtggatccgc accgtgtcgc
     3901 tcgccgtcgg ttgggcgccg ttccccggct ggagctcgaa gtagatcatg ccgctgtcgg
     3961 tcttggtggc accgtccttg gccgccatct cgtcgcggaa cttcgccccg gccgtcttct
     4021 cgcgatcggc gaccgcggcc atcttgccct gcatgaacgc gtcgagcttg gcggcgtaga
     4081 ccgccatgtc gaccttgtgc tcgttgccct gcaggtggtc ggtgatcccg gcttggatca
     4141 tcgcgatctc ctccgttgtc ggatcgaacg cgggcacctt ggccgccaac gccatgccga
     4201 gcgcgtacag cagcttctgg tcttctgttg cggggtcggc ggcgccggct cccgcgaacg
     4261 cgaacagcag cgcgccaatc gccgatgaca gaatcaagcg tcgcatgtag ggattccttc
     4321 ttgacgtccg ctggtcggaa gggcctcggc aacccgctac gggccgcccg tcgcatccgg
     4381 caccggaagg gctactttac cagagatccg gcccgccggc cgccggcggc ggctccgtcg
     4441 atcacggcgg cgacacagaa cagcgcggaa aacgaggcgg ccagcgcgac ggcggccggt
     4501 agcacgccgt cggcgagcca ggccggacgc cacagctcga gcccgaggtt gaccacgagc
     4561 ccggtggcga tcgacgctgc tgccgccgcc gcgccgactc gccgccagtg gaagccgacg
     4621 gccagcgccg gggccagcgc cgcgccgaac gtgccgaagg ccatcgtgcc gagcatcgcg
     4681 atcaaaccgc cgtgacggaa cgcgagcagc gcggccaagc cggccacgat caaaaccgcg
     4741 gcgcgcccgg cggcgagcgg ctcgggccgc ccgaggccca gcacccgcgg caggtcgcgc
     4801 acgagcgcga cggcgccgat gttgagcaac gagtcggcgg tcgacatgat cgccgccagg
     4861 atcccggcga cggcgagccc cgcgaccagc tccggtgtga actcgagcag gaaccgcacc
     4921 gccgcctcgt ccgcgcgctc gagcggcgcg agccggccac ccgccaccag cgccggcacg
     4981 gccaggccga cgccgagcca cagcagtacg cagaccgctt ggctgccgga caggaccagc
     5041 ggaaaccagc gcagcttttt cgggtccgag atcatgtaga acttgtgcag catctgcggc
     5101 tggcccaaca cgccgacgcc gaagacgaag aagaagccga acacggtcag cggtgcggca
     5161 cgccccagcg gttcgaggaa ctgcggtccg aacgaggcgg attcggcgac ggcggcgacc
     5221 gtccggtgcc atccgccggc ggcggcgacg gcgtggagga aaaccacgat cccggtcagc
     5281 atcatcaacc cgccctggac cagatcggtg tagacgccgg cgaccatccc gccgaggacg
     5341 ctgtaggcca cgagcaccgc gaggccgagc agcatcgcac tggccccgcg ctcgaggccg
     5401 aagaccgcct gcagcagcac gccgagggcc tgcagctgca tgccgagata agccaccgtg
     5461 ccggacagca ccgccagcgt cgccagaccg gtcacgaccc ggctgccgaa acgcgcgtcg
     5521 aacgcgtcgg gaacgctgta gatcggctgc tcggccgcca gcgcacgcag tcgcgtgccg
     5581 agcacgcggc acagcaggcc gccggtgaag ccgagcggca gcacgatcat caacgacgcg
     5641 agaccgatgc ggtaggtcag cccgggcccg ccgagaaaga cgaatccgct gaacgccgcc
     5701 gacatcgtcg cgagcgccgt gacccacaga ccggcccgct ggccggcgat gaagaagtcg
     5761 cgggcatcgc gagtgcggcc gagcgaccag aggccgacgg ccgtgacgat cgccaggtag
     5821 cagacggtcg cgacgacgat cagcgggcgg tcggcgaagc tcatcggcgc gatcgcgtgg
     5881 cgtccggccc ctcgtcccgc cagagccagg caaagcccca agggacgagc agcaacggca
     5941 cgataccgag gccgacgagc atccagaccg tcgcggccgg caggcccagc cagccgcccg
     6001 tccgcccggt ggcgaacagt atgccgagcg tcccggcctc gagcgatagc cagagcagcc
     6061 cgagccagac gacgacgccg aaacgcctcg gtccgccgtc acgcccgagc gccatcaacc
     6121 cgaccggcac gatcggcgcg acgacggcga agacgaagcc gccggcctcg ccgccgacga
     6181 gcaacgcgag cagcagcacg ggagaggaga tcgcgagcgc gatgagggag aatcgtgcca
     6241 tttccgcgag gccattctga cacagccgct cccttccggg cccgcgggcg atttgctagg
     6301 atcgagcccc tcggaagcgg attcgcctgg agccccgttt gcagctcgga ttcgtcatcg
     6361 accacacgcg ctgcatcggc tgccacgcct gcaccgtcgc ctgcaaggcc gagaacgatg
     6421 tcccgctcgg cagttttcgt acctgggtca agtacaccga gcgcggggac ttcccccaag
     6481 tgcggcgttc gttcgccgtg ctgcgctgca accaatgcac cgacccgccg tgcgtgacga
     6541 tctgcccggt cggcgcactg gggaagcggc gcgacgggat cgtcgacatc gatccgcagc
     6601 gctgcatcgg ctgcaagagc tgcctgcacg gttgtccgta cgatgcgctc tacatcaacg
     6661 actcgacggg gctggcggag aagtgtcact tctgtgcgca tcgcagcgag atcgggctgg
     6721 caccggcttg cgccgtcgtc tgcccgaccg aggcgatcgt ccccggcgac ttcgacgatc
     6781 cccgaagcca cgtggcacgg ctcgccgccg agggcggcct cgaggtgcgc aagcccgagg
     6841 cgggcaccgg tcccaacgta cgctaccgcg aggtggaccg cgcgggcatc gacccgctcg
     6901 agacgaacgc cgcgggcggg ttcctctggg ccgagcagct tcccggcgct cggctcgacg
     6961 cggcgcggtt cgaggcggtc gagcggccgt ccgcggcgcg aacgacctac gacgtcgaac
     7021 atcctccggc atggggcgcg gcgatcaccg gctacctgtt cgcgaagtcg atcgcggccg
     7081 gggcattcct cgcggcgatc ccgtggctcg gtccgtcggc cggcgggtcg ccggccgctg
     7141 cccgcccggc gctggccgcg gtgctgctgg cgctcgcggc actcgcggcg acgggcgcgc
     7201 tgctgatcgc cgacctgaag cggcccgagc gtttctggtt cctgctgcgt tacggcaacc
     7261 cgtcgtcgtg gctcgtgcgc ggcacgtggg tgttgatcgg ttacggcgcg ctgctcgtgg
     7321 tctggctgct gttgctcgga accggcaatg ttccctcggc gccgctcggg ccgtggctcg
     7381 tcgtcgcgac gtcgctgctt gcggcgctga ccgcctgcta cacggctttc ctcttcgcgc
     7441 aggcgcgcgg gcgcgtgctt tggatgggcc gcgggctgtg gctcggcctg ctggcccacg
     7501 ccgcgatcgc cggcggagcg gcgctgatcc cgctcgccgc cgcgctcggg tcgggcctcg
     7561 agacgatcgc gccgctgcgc cggctgctgg tcgtcggcct gctcgtgcat ctgtcgctga
     7621 gcctcgccga gcgggcgttc gccccgcgcg gtcgcgagga agagtaccgg cgggccgtgc
     7681 gcctcgtcag ccacggcccg ttcgcccggg cccactggtg gatcggcgtt ggcgccgggg
     7741 tcgtgctgcc gatcgcgctg ttggtcgtgc cgtcgagccc ggcggcctgg accgccgcct
     7801 cggt
//
LOCUS       DASPGN010000210         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1207813, whole genome shotgun sequence.
ACCESSION   DASPGN010000210 DASPGN010000000
VERSION     DASPGN010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207813"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..714
                     /locus_tag="VD788_05965"
     CDS             94..714
                     /locus_tag="VD788_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXV75849.1"
                     /translation="MERARFDGGCRVKDLDKTAMGKRIRQIRLGAGLRQWELARMLGT
                     TQSAVHKYEHGVVPEPRRLVELARIGCTSIEWVLTGHHWDNGSEVQERLSPEILRTAT
                     ALREISDEQRATVDEALGIMREAVRALESGPDRRSPAYVAVLSDSFGEHAGETLRLLE
                     SAWKIQRSVLRCVTREAGRRLDTHRVPARNGGKPSSGDSDGAASAS"
     gene            complement(711..2276)
                     /gene="lysS"
                     /locus_tag="VD788_05970"
     CDS             complement(711..2276)
                     /gene="lysS"
                     /locus_tag="VD788_05970"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HXV75850.1"
                     /translation="MASETRSIDGAVTTRHWADEIADAVRGSGPPVISTGISPSGEIH
                     IGNMREVLTADAVFRALRDQGVDARFNYVADNFDPLRRVYPFLDAETYAPLVGRPLSE
                     IVCPCGEHASYSEHFLRPFLAALDELGVTVEVERADEMYKSGRMSPYIIRALERRDQI
                     ATILSELTGKRVDDDWSPFNPLCPDCDRITQAKVDGFDVVKRLVHYSCACGARGSVPM
                     AGGGKLVWRIDWPARWILLGVTVEPFGKDHASDGGSYDTGVRIVREVFGGEPPYPVPY
                     EWIRLKGQGDMSSSRGNVLSIGAMLEVVPPEALRYLVIRERPTRAINFDPGLPLLQLV
                     DELDDVEARGRNERAVELSRAGEFAAVGVPFKHLVVVAQSARFDAESVLAALRRSGYG
                     ELDREAVETRLAYARRWLERFAPEEIRFVVAERLPAEASALTPPQRRFLATLADRIEG
                     MSEGKRIHEIIYDLAPSVDGATSGELFEAIYLALLGKRRGPRAGLFIASIGWEFCAAR
                     FREAAAAVVPGGV"
     gene            2444..3160
                     /locus_tag="VD788_05975"
     CDS             2444..3160
                     /locus_tag="VD788_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV75851.1"
                     /translation="MTLREAAELTRRSITTLRRHIRSGRLHAEKRYGRFGPEYFVSEQ
                     DLTDAGLDTEVRALDRVSARGSSPATSQPTGSELASYAVGGGWLRDIVPLSLYQELQM
                     KHEQLLVQYGMVRAGGLRAMEIRAELDRTQQQLDRAGQESARVQRALRDANSRLESDL
                     REARLELEGRQLVIAALEEKTRALEMLTRNAVTNEALDVQLEELRQQRLRVDDLKQRG
                     GPEASPASDGRPPADKTLDH"
BASE COUNT          545 a         1102 c         1089 g          462 t
ORIGIN      
        1 gaatcggagc cggtcgttcc attgtgacta gcgaaaccgg cgcaagacgc cccggttcct
       61 tgacttttgt cgaggtcgac acagattcga gccatggaac gagcgcgttt cgacggcggg
      121 tgtcgcgtga aggacctcga caagaccgcg atgggcaaac ggatccgaca gatccggctc
      181 ggcgccggct tgcggcaatg ggagctggcc cgcatgctgg gaaccaccca atcggcggtg
      241 cacaagtacg aacacggcgt cgtgcccgag ccgcgacggc tggtcgagct ggccaggatc
      301 ggctgcacca gcatcgagtg ggtgctgacc gggcaccatt gggacaacgg ctccgaggtc
      361 caggagcgac tctcccccga gatcctgcgc acggcgacgg cgctgcggga aatcagtgac
      421 gagcagcgcg cgaccgtcga cgaggcgttg ggcatcatgc gcgaggcggt gagggcgctc
      481 gagagcgggc ccgatcgacg ctcgccggcg tacgtcgcgg tcctgtcgga ctcgttcggc
      541 gagcacgccg gtgagacgct gcgcttgctc gaatcggcct ggaagatcca gcgctcggtc
      601 ctgcgttgcg tcacgcgcga ggccggccgc cgcctcgata cccaccgcgt cccggcgcgg
      661 aacggcggca agccgtcgag cggggactcg gacggcgcgg cgtcggcgag ctagacccca
      721 cccggaacga ccgcggcggc cgcttcgcga aagcgcgccg cgcagaactc ccagccgatc
      781 gatgcgatga acaggccggc gcgtggcccg cgccgcttgc cgagcaacgc gaggtagatc
      841 gcttcgaaca actcgccgct cgtcgcaccg tcgacgctcg gggccagatc gtagatgatc
      901 tcgtggatgc gcttgccctc gctcatcccc tcgatccgat cggcgagcgt ggcgaggaat
      961 cgccgctgcg gcggggtcaa cgcggacgcc tcggccggca atcgctcggc aacgacgaac
     1021 cgaatctcct cgggcgcgaa gcgctcgagc cagcgccgag cgtaggccag ccgcgtctcg
     1081 accgcctcgc gatccagttc accgtacccc gagcgcctca gcgccgcgag gacggattcc
     1141 gcgtcgaagc gcgcggattg cgccaccacc accagatgct tgaacggaac gccgacggcc
     1201 gcgaactccc cggcgcggga cagctcgacc gcgcgttcgt tgcgcccacg cgcctcgacg
     1261 tcgtccagct cgtcgacgag ctgcagcagg ggcagaccgg ggtcgaagtt gatcgcccgg
     1321 gtcggtcgct cgcggatcac caggtagcgc agggcctcgg gagggacgac ctcgagcatc
     1381 gccccgatcg acagcacgtt gccgcggctc gacgacatgt ccccctggcc cttcagccgg
     1441 atccactcgt acggcacggg gtacggcggc tccccgccga acacctcgcg cacgatccgg
     1501 acgcccgtgt cgtacgagcc gccgtcgctc gcgtggtcct tgccgaacgg ttcgacggtg
     1561 acgccgagca gtatccagcg agccggccag tcgatgcgcc agaccaactt accgccgccc
     1621 gccatcggca cgctgccgcg ggcgccgcag gcgcagctgt aatggacgag ccgcttcacc
     1681 acgtcgaagc cgtcgacctt ggcctgggtg atccggtcgc agtcggggca gagcgggttg
     1741 aacggcgacc aatcgtcgtc gacacgcttt ccggtgagct cgctcaggat cgtcgcgatc
     1801 tggtctcgtc gttcgagcgc gcggatgatg tacgggctca tgcgccccga cttgtacatc
     1861 tcgtccgcgc gctcgacctc gacggtgact cccaactcgt ccaacgccgc gaggaacggc
     1921 cggagaaagt gctcggagta gctcgcatgc tcgccgcacg ggcagacgat ctccgacagc
     1981 ggccgcccga ccagcggggc gtaggtctct gcgtccagaa acgggtagac ccggcgcagc
     2041 ggatcgaagt tgtccgccac gtagttgaac cgcgcgtcga cgccctgatc acggagcgcg
     2101 cgaaagaccg catcggcggt caacacctcc cgcatgttgc cgatgtggat ctcgcccgac
     2161 ggcgaaatgc cggtcgagat caccggtggc ccgctccccc gcaccgcgtc cgcgatctcg
     2221 tcggcccagt gacgcgtggt caccgcgccg tcgatcgatc gtgtctcgct cgccatcccc
     2281 gtcgtctccg tcggcgcagg atacatggtg cgcgagcgta gtcgcaatcg gttccgccgt
     2341 gcgacggttg tgcgcgtcgt caagactgtg atacaagacg tccgaacagc cggttgccgg
     2401 cgtcgaggcg caagcggcgc cggggatcga atcgttgagg cgaatgaccc tacgcgaagc
     2461 agccgagctg acgcgccggt cgatcaccac cctgcggcgt cacattcgca gcggcagatt
     2521 gcacgcggag aagcgctacg ggcgtttcgg acccgagtac ttcgtttccg agcaggatct
     2581 gaccgacgcc ggcctcgaca ccgaggtgcg cgcgctcgac cgggtgtcgg cgcgaggcag
     2641 ctcgccggca acttcccagc cgacgggctc cgagctcgca tcctacgcgg tcggtggcgg
     2701 ctggctgcgc gacatcgtcc cgttgagcct gtatcaagag ctgcagatga aacacgagca
     2761 gctgctggtc caatacggaa tggtccgcgc cggaggtctg cgcgcgatgg agatccgcgc
     2821 cgagctcgac cgtacccagc aacagctcga tcgggcgggt caggagagcg cgcgtgtcca
     2881 gcgagcgttg cgtgacgcga acagccggct cgaaagcgac ctgcgcgaag cgcggctcga
     2941 actcgagggg cgccagctcg tgatcgcggc gctcgaagag aagacgcgag cactcgagat
     3001 gttgacacgc aacgccgtca ccaacgaagc gctcgacgtg caactcgagg aactgcgcca
     3061 gcagcgcttg cgcgtcgacg atctcaagca gcggggtggg ccggaggcct caccggcgtc
     3121 cgacggtcgt ccgccagcgg acaaaacgct cgatcactga cggtcgcctc gtcgcgcggc
     3181 tcgagcttgc gcattgcg
//
LOCUS       DASPGN010000211         4156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_387827, whole genome shotgun sequence.
ACCESSION   DASPGN010000211 DASPGN010000000
VERSION     DASPGN010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387827"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VD788_05980"
     CDS             complement(<1..212)
                     /locus_tag="VD788_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75852.1"
                     /translation="MRHRPWLVVSWGAGLLLLVACGAGDAERGREGRAAGVGRWALAI
                     HGGAGVPRESVDATRQREYLDALERAL"
     gene            complement(314..1210)
                     /locus_tag="VD788_05985"
     CDS             complement(314..1210)
                     /locus_tag="VD788_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HXV75853.1"
                     /translation="MVDGRRAENSVLSQYFSEIREYPLLTKDEEKNLARDIQNGKREA
                     LNELVESNLSFVAKVASEYRSLGLPFEDLLNEGNVGLIEAAKRFDASKDIKFISYAIW
                     WIRKAILKALSEQSHTVRLPYSQMKKVKEIREAERMLRRELNRKPSREEISLHLAKSV
                     SKIDKVLQHTAHEVSIDEPVGEEKETPMWECLVDDRGASPEERLLDRELTDGIEEVYE
                     HLNDQQKTVIAHRFGLGGSPPLTLQETGDRMRLSRERVRQIECQAKERMRRIFRKMRS
                     IDSPAKGGSRTSVRAPVRRPRR"
     gene            1460..3316
                     /locus_tag="VD788_05990"
     CDS             1460..3316
                     /locus_tag="VD788_05990"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75854.1"
                     /translation="MDRSSIVNRIGTAVGRGRRWIMRRLLGTSCLFVYGRRYGVDLPE
                     GAFDQQRAGRILDFLLTERLTDRRHLRRVSRPASVRQLRRVHSDVYLDSLDREDAVSG
                     IVGLHLDEAQRDAFLSSQREQVGGTILACRLALAGSGVVANLGGGFHHASATRGQGFC
                     AYNDVAVAVADRRAAGFDGRVLVIDLDLHDGDGTRRIFADDESVYTFSIHNRTLGDER
                     AVASTCLALGDDVADQAYLSAVRARLLPLLPRLEPRLAIYLAGADPAHDDRLGNWRIS
                     DRGLLERDRLVVDCLRRTARPVPLVILLAGGYGSHAWRHSARFLSTLASGGRPVEPPR
                     TAQLRLAHYRRVLRLLREPELTHEPTDSFRLTEEDVDPGAAVFSRSQRFLGFYSRHGL
                     ELALERTGILDKIRRRGLGGLELELLPGSPGEHTMIVRSGEHDDQSVVELRARIDRST
                     VAGMRLLWLEWLLLQDPSAGFSDDRPPLPGQRYPGLGLLGDVSALLVLICERLELDGM
                     AFVPSHYHVAAQARSYFRFIDPSAQARFLALQRLLEPRSLAAATAIIERGEIRDGTVP
                     EPVGWRPEPMVYPVSEALRRRFDADDYRRTVNAELGRLRFHFHEPEAEGHEA"
     gene            complement(3304..3576)
                     /locus_tag="VD788_05995"
     CDS             complement(3304..3576)
                     /locus_tag="VD788_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75855.1"
                     /translation="MAPPNRSIRATDIDSLRLIALGRIPRCGSCARFRALVRAHDEGE
                     LGTALPAVPKHCTHRVCQPLVDRLLAREAPRGGRNVFGGAAYPYAS"
     gene            complement(3670..>4156)
                     /locus_tag="VD788_06000"
     CDS             complement(3670..>4156)
                     /locus_tag="VD788_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV75856.1"
                     /translation="PGTRETLEGLAGLCYREVFEMREFDLRYHELPVAVDNGVAVGSG
                     LVLDTLPMRHHPSSIGLRFRLAGSGGDAVVAVSGDTGWCDNLERLARGCDLLLLECTS
                     VEPLTDVHISLRELRDKADRLDATRIVLVHLDDAVAADLSLDPIPRVGAATDDLTIEF
                     P"
BASE COUNT          595 a         1429 c         1413 g          719 t
ORIGIN      
        1 agcgcccgct cgagcgcatc gaggtattcc cgttgtcgcg tcgcgtcgac ggactcgcgg
       61 ggaacgccgg cgccgccgtg gatcgccagc gcccaacggc cgacgcccgc tgcgcggccc
      121 tcgcgcccgc gctcggcgtc cccggcgccg caggccacga gcaggagcag acccgcgccc
      181 cacgaaacga cgagccacgg tcgatgacgc atcttcggcc ccctcgatcc ggcgcgatgc
      241 cgaacgcgac cgatgatacg gaggtgggcc ggcggagtcc aatcgcgacg gcggacagcc
      301 cgccggcggc gtctcagcgt ctcgggcggc gcaccggggc gcgaaccgag gttcgcgacc
      361 cgcccttggc cggcgagtcg atcgagcgca tcttgcggaa gatccgccgc atacgttcct
      421 tggcctgaca ttcgatctgc cgtacgcgct cacggctgag gcgcatgcga tccccggtct
      481 cctgcaacgt gagcggcggg gagccgccga gaccgaagcg gtgcgcgatc accgtcttct
      541 gctgatcgtt caggtgctcg tacacttcct cgatgccgtc ggtcaactct cggtcgagca
      601 agcgctcctc gggcgaggcg cctcggtcgt cgaccagaca ttcccacatc ggcgtctcct
      661 tctcctcgcc gaccggctcg tcgatgctga cctcgtgggc ggtgtgctgg agcaccttgt
      721 cgatcttcga cacgctcttg gccaggtgca gcgagatctc ctcccggctc ggcttgcggt
      781 tcagctcgcg ccgcagcatc cgctcggcct cgcggatctc cttgaccttc ttcatctgcg
      841 aatagggcag gcgcaccgtg tgcgattgct cggacaacgc tttgaggatc gccttgcgga
      901 tccaccagat cgcgtagctg atgaacttga tgtccttcga ggcatcgaat cgcttcgctg
      961 cttcgatcag cccgacattg ccctcgttca gcaggtcctc gaacggtagc ccgagcgacc
     1021 ggtattcgct cgccaccttg gccacgaagc tgaggttgga ttcgaccagc tcgttcagcg
     1081 cttcgcgctt gccgttctgg atgtcacggg cgaggttttt ctcctcgtcc ttggtcaaca
     1141 acgggtactc gcgaatctcg gaaaaatact gcgagagcac ggagttttct gctcggcgtc
     1201 cgtccaccat cgtgcacctc tccgtctcgc cctacttgca cgtccgcatc gtcgatcgcg
     1261 ggcggtgtcg tgaatgtaga agtgctcgtt cgcgcttgcc atggcgattc gcgtaaagcg
     1321 cgcgtggcgt cgtcaggttg tcggcctatt cggttttgac ggcgcggtcg tgcagtgcgg
     1381 gcgacggccg tcgctcgaag aatgtgaggc cgaatgcccg cgcgccgacg gctataatgc
     1441 ccgcgccgag gcgtcgtgaa tggaccgcag ctcgatcgtg aaccgaatcg gcacggccgt
     1501 cggacgcggc cgccgctgga tcatgcgccg gctgctcggc acgtcctgtc tgttcgtcta
     1561 cggcaggcgg tacggcgtcg atctgccgga gggcgcgttc gaccagcagc gcgccgggcg
     1621 tatcctcgac ttcctgttga ccgagcggtt gaccgatcgc cgccatctgc ggcgcgtatc
     1681 gcgaccggct tcggtccggc aactgcggcg ggtgcacagc gacgtctacc tcgactcgct
     1741 cgatcgcgag gatgcggtga gcgggatcgt cggcctacac ctcgacgagg cgcagcggga
     1801 cgccttcctg tcctcacagc gagagcaggt gggcggtacg atcctggcct gtcgcctggc
     1861 gctggccgga tcgggggtcg tggccaatct cggcggtggg tttcaccacg cgtcggccac
     1921 tcgcggccag ggcttctgcg cttacaacga cgtcgcggtc gcggtggccg accgccgggc
     1981 ggcggggttc gacggacggg tgctggtcat cgatctcgat ctgcacgacg gcgacggtac
     2041 ccggcggatc ttcgccgacg acgagtcggt ctataccttc tcgatccaca atcgcacgct
     2101 gggagacgag cgggcggtcg cgtcgacctg cctcgcgttg ggcgacgacg tggccgacca
     2161 ggcctacctg tccgccgtgc gggcccggct gctgccgttg ctgccacggc tcgaaccgcg
     2221 gctcgcaatc tatctcgcgg gtgccgaccc ggcccacgac gatcgtctcg gcaactggcg
     2281 gatcagcgat cgcgggctgc tggagcggga ccggctggtc gtcgactgcc tgcgccggac
     2341 ggcgcgtccc gttccgctgg tgatcctcct ggccggcggc tacggttcgc acgcatggcg
     2401 tcacagcgca cgcttcctct cgacgctggc cagtgggggg cgtccggtcg agcccccgcg
     2461 gacggcacaa ctgcggctgg cgcactatcg ccgcgtcctc cgtctgctgc gcgagccgga
     2521 gttgactcac gagccgaccg actccttccg attgaccgag gaggacgtcg atccgggggc
     2581 cgccgtgttc agccggtcgc agcgcttcct cggcttctat tcgcgacacg gcctcgagct
     2641 cgcgctcgaa cggaccggga tcctggacaa gatccgtcgc cgcggcctcg gtggactcga
     2701 gctcgagctg ctgcccggat caccgggcga acacacgatg atcgtccgca gcggggagca
     2761 tgacgaccag tcggtggtgg agctccgggc gcggatcgac cgctcgacgg tcgccggcat
     2821 gcggctcctg tggctcgagt ggctgctgtt gcaagacccg tccgccggtt tcagcgacga
     2881 ccgacccccg ctcccgggac aacgctatcc cggactcggc ctgttgggtg acgtctccgc
     2941 gttgttggtc ctgatctgcg agcgactcga gctggacggc atggccttcg ttccctccca
     3001 ctatcacgtc gcagcgcaag cgcggtcgta ttttcgcttc atcgacccgt cggcccaggc
     3061 gcgcttcctc gcgctgcagc gcctgctcga gccgcggagc ctcgcggcgg cgaccgcgat
     3121 catcgagcga ggggagattc gcgacggcac cgtccccgag ccggtcggct ggcgacccga
     3181 accgatggtc tacccggtga gcgaggcgtt gcgccggcgt ttcgatgcgg acgactaccg
     3241 tcgcacggtc aacgccgagc tcggccggct acgcttccac ttccacgagc cggaggccga
     3301 gggtcacgag gcgtaggggt aggcggcccc gccgaagacg tttcgcccgc cgcgcggggc
     3361 ctctcgcgca agcaggcgat cgacgagcgg ctggcacacg cggtgcgtgc agtgcttggg
     3421 taccgcgggc agcgcggtcc cgagctcgcc ctcgtcgtgt gcccgcacca gggcgcggaa
     3481 gcgggcgcaa ctgccgcaac gcgggatccg gcccagggcg atcagccgga gcgagtcgat
     3541 gtcggtggcg cgaatggatc ggttcggggg ggccatcgag ttcctcctcg gcggggccat
     3601 gtcaaccgtg ctgcctatag aacgcgcgac ggcgccgttt ggttgcgcac aagctttccg
     3661 cgtgcggttt caggggaatt cgatcgtcag gtcgtcggtc gcggcaccga cccgcggaat
     3721 cggatcgagc gacaggtcgg cggcgacggc gtcatcgaga tgcacgagca cgatcctggt
     3781 cgcgtcgagc cggtctgcct tgtcacgtag ctcgcggagg gagatgtgca cgtccgtcag
     3841 cggctcgacc gaggtgcact cgagcaggag cagatcacaa ccacgcgcga ggcgctcgag
     3901 attgtcgcac catccggtat ctccgctgac cgcgacgacc gcgtcgcccc cgctgccggc
     3961 gagacggaac cgcaggccga tcgagctggg gtgatgccgc atcggcaggg tatcgagcac
     4021 gagcccggac ccgaccgcca cgccgttgtc gacggcgacc gggagttcgt ggtaacgcag
     4081 atcgaactcg cgcatctcga acacctcccg gtagcacagt ccggccaatc cctcgagcgt
     4141 ttcccgcgtg ccggga
//
LOCUS       DASPGN010000212         9039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_505329, whole genome shotgun sequence.
ACCESSION   DASPGN010000212 DASPGN010000000
VERSION     DASPGN010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505329"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VD788_06005"
     CDS             complement(<1..215)
                     /locus_tag="VD788_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75857.1"
                     /translation="MEFNPDATDLEEAFFARENARLLEQLRIKTEQAERRALLRRVVD
                     IDDDAFLDRLITLGIGPERAMVLRLTPL"
     gene            complement(303..842)
                     /locus_tag="VD788_06010"
     CDS             complement(303..842)
                     /locus_tag="VD788_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HXV75858.1"
                     /translation="MKRWVFCLVASAICVPLPASAAGGYLGGSWLSTSAEFDTAVENF
                     DTDESGWKVFAGFDAERFFGVEVSYRDMGDFSEVHGLDSLDADIDAWEASLRGLLPLG
                     RVLQLFAKFGYANVSFDGDVATGSIVSADVDADGWDLIYGVGIDLNLGERFGLRAEWE
                     EFDVEDSLNSFSAGAFFRF"
     gene            complement(874..1788)
                     /locus_tag="VD788_06015"
     CDS             complement(874..1788)
                     /locus_tag="VD788_06015"
                     /inference="COORDINATES: protein motif:HMM:NF022892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 domain-containing protein"
                     /protein_id="HXV75859.1"
                     /translation="MTRPRSTALCLGLALTIGCSSGPSRQERFATPEQAAEALVSAAE
                     RFDVSALANILGPEGIDLVVTEDRVQDRNQAQAFAAEARTQMRVERDPESSTLAVLSV
                     GPDDWPLPIPLVEEGGGWRFDSEAGREEILNRRIGQNELDAIDICRGYAEAQHEYASE
                     KHDGSNLHQYAQRIISTPGRHDGLAWQEPDGTWAGPVGEGIARVIAEGYTDRIEPYHG
                     YYFKILKGQGPSAPLGAMDFRIKDVMIGGFALVAAPADYGVTGIMTFIVSHTGVVYEK
                     DLGPETEAQFRAMELYDPDETWTTVEEL"
     gene            complement(1785..3074)
                     /locus_tag="VD788_06020"
     CDS             complement(1785..3074)
                     /locus_tag="VD788_06020"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HXV75860.1"
                     /translation="MKPNLLRFRRGLAIVSCAALLGSSGGGPLLADEPQPAASPAPTP
                     VASPAPEVTKIPSDQLDSLVAPIALYPDPLLAQVLVASTYPLEIVQLQQWMTKHPELK
                     DQALADEVAKQPWDPSIQSMAATPEVVKRLAEDIQWTTDLGNAFLAQQSDVMDAVQRM
                     RKKAKDNGALESNEQQNVETKQVDGVDVIIVESSNPEVIYVPSYSPTVVYGPPLYPYP
                     PIYYPPYPPGAAFVSFSFGVMWGAAVWGGSCCGCGWGGNDIDINVDNNFNRIEHNRNG
                     DRGGGGRGGSGNKWQHNASHRGAAPYGDKATANKYRGSARGESRAGQPSVSNRAGGAG
                     ASNRAAGPGASNRAGGSSASNRAGGPGASSRAGGAGGDKIGNRSIPQSTGSRAHSGFG
                     GGSRGYSGSGARASSSRGSSSMSRSGGTRGGGRGGRR"
     gene            complement(3242..5566)
                     /locus_tag="VD788_06025"
     CDS             complement(3242..5566)
                     /locus_tag="VD788_06025"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HXV75861.1"
                     /translation="MMLELAIKSTLVLLAVLLGGLVGRSSAAARHLLRASGLVALLVL
                     PALAVVLPAWELPLAGPRSALAAGATATSPAFDPPADLDEPAEPRVAASYRREPVVDE
                     PATAAVSTAGVAAPREVAPRSKPAPLAWVPWLWGVGAAFVLLRLGVGWFRVNRIAIEA
                     TPLVHGPARQALERCARQVGLARPPRVLTTDALRLPVLWGFVDPTLLLPRTACDWDRG
                     RLDAVLLHELAHRRRADGLWLLLGRLSVALWWFHPLAWWVERAARADCERAADDVVLA
                     CGERPSRYAGQLLAIAGQLPARAPASTLGIAERNQLAARVRSILSADARRGLRRRGAW
                     VLSSLSVVAVVSIASAQWAERAEASTPERIASPSAATQAVRIDDDDRGAIMLAHDRPG
                     DGERRFREALALHRQERWDEAAAGFIAAADEGYREAVSLYNAACGYARLGQAERAVEL
                     IERAVDAGFDDPELIASDRDLDPIRREATFRRMIDELPGAHRASGGRYEGAVDRYDDL
                     VAQDSTDGRAWYDVGSALLGMRESDRAIDALDRAARMLDGNANALYNLACAYSLAGDE
                     RQALDHLERAVAAGFDSEERFRNDSDLDGIRETARFAEIERLHDRLSLARFRSWWNWG
                     SDYSARRWAPAVEDYTELVGEQPGLGRAWSNLGWGLHHSRRFGEAREAFLEQYELGYQ
                     RRLAAYNVGCTYAMEERPDEALDWLGKALDAGSISVGQLLGDDDLRGLHDDPRFDALL
                     ERAADDEDDAEDRREYRRILDDRRRRSHDGARLY"
     gene            complement(5563..5958)
                     /locus_tag="VD788_06030"
     CDS             complement(5563..5958)
                     /locus_tag="VD788_06030"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXV75862.1"
                     /translation="MRKKQQEGSRLSRREREVMDIIYRAGEATVTEVLEAIEQPPSYS
                     AVRSTLNILEAKGHLRHRHEGNRYVYLPTRTRAEARRSALVHVLDTFFDGSAADAVTA
                     LLEEQGTELNQEEIERLSRLIRQARREGR"
     gene            6101..6658
                     /locus_tag="VD788_06035"
     CDS             6101..6658
                     /locus_tag="VD788_06035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV75863.1"
                     /translation="MTTVNDSVLMTDVRDGRAAALGTLFERHHARLYRFCLRMTGNRS
                     VSEDLVQEIFMRILRHRATFRPGTAFVPWMYRIARNACIDHLRRGAAGGEALEEEETL
                     VAGGPSVEETVERGRAAQLMRRALLRLPVERRAVLLMSRYEFKTYEEIARTLDCSVSA
                     VKIRAYRAIKQLREVYCDLAREAAP"
     gene            6655..7449
                     /locus_tag="VD788_06040"
     CDS             6655..7449
                     /locus_tag="VD788_06040"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV75864.1"
                     /translation="MNCDETTGRVSDRLKRLLDPRSEADLERHLSRCDDCRAEAEAIE
                     RVWNEMGRLDDEVPSDRMRSRFHDALAAYERRDAGPAPARGSGRRLGALWARLPGAQL
                     ASMAAALSVGMLVGGFWLAPGTGEIEALRFELRSMERAISLTLLEHGSASERLRGVQF
                     SARVEPDSRVVEALLDTVAADPNVSVRLAAVEALAPLLDRRPIAERLVETMAEQSSPL
                     LQVTLAEVLLDAGIEGSRPAVLRLIEHEGTDPAVREHLRGVMRNSG"
     gene            7469..8320
                     /locus_tag="VD788_06045"
     CDS             7469..8320
                     /locus_tag="VD788_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75865.1"
                     /translation="MKKVTATLIAALALGATAGAGELVERRTIRDAMAVAGDGRTGEL
                     VVDNVFGSIRVETHDRPLIEYVAHETIEARDRDAVDRAREEVRLDVRREGDSYTLFVD
                     GPFRCDDDRRCHCGRETDYVVVYDFEIRVPRRIDVDLRTVNGGEIEVTGVRGDYRVAN
                     VNGGVRLLHVDGSGRANTVNGPIRVVFDGNPRAESEFRTVNGDVDLEFQPDLSADVRA
                     KTFNGEIWTDFDAEPLPLDAPQTARRDGKTVIRTDRWAALRIAGGGPLLTFETLNGDI
                     LIRNALR"
     gene            8338..>9039
                     /locus_tag="VD788_06050"
     CDS             8338..>9039
                     /locus_tag="VD788_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXV75866.1"
                     /translation="MFRRVILPTIVLIPIVIATPGAAAQQGEGQRLVVPISDPSRPIV
                     LRASLVSGGIVVTPHDGREVIVIARPRDAELDARRADGMRLIPNTSVGVTAEEEANRI
                     DVQADWSSRGIDLEIQVPVRTSAELSTVNSGDIVVRGLTGDLELQNTNGSIVAEDIDG
                     SVVANTTNGTVRVRFDRVKPDTDMAFNSFNGDVDLTFPASLEAEFRISVGRGEIRTDF
                     EAVQRPASPKVEQDGS"
BASE COUNT         1359 a         3207 c         3130 g         1343 t
ORIGIN      
        1 agcggcgtca gccgcaggac cattgcgcgc tcgggcccga tcccgagagt gatcagccgg
       61 tcgagaaacg cgtcgtcgtc gatgtcgacg acgcgccgca gcagggcgcg acgctccgct
      121 tgctcggtct tgatccgcag ctgctcgagc aaccgcgcgt tctcccgggc gaagaacgct
      181 tcctcgagat cggtcgcgtc ggggttgaac tccatggtat tcctccgtgc ttgcggcgat
      241 tcgtcgggcc gcgccgcccc aggatacacc ggcccgcccg cggctcgcgc ggcgccgcca
      301 cgctagaacc ggaagaacgc tccggccgag aacgagttca ggctgtcctc cacgtcgaac
      361 tcttcccact cggcgcgtag cccgaagcgc tcgccgagat tcaggtcgat ccccacgccg
      421 tagatcaggt cccagccgtc ggcgtcgacg tccgcgctga cgatcgaacc ggtcgccacg
      481 tcgccgtcga acgacacgtt ggcgtagccg aacttggcga acagctggag cacgcgtccg
      541 agaggcagga ggccgcgcag cgaagcctcc caggcgtcga tatcggcgtc gagcgagtcg
      601 aggccgtgaa cttccgagaa atcgcccatg tcccggtagc tgacctcgac gccgaagaac
      661 cgctccgcgt cgaaaccggc gaacaccttc caaccggact cgtccgtgtc gaagttctcg
      721 accgccgtgt cgaactcggc ggacgtgctc agccagcttc cgccgagata cccgccggcg
      781 gcggaagccg gcagcggaac gcagatcgct gacgcaacca ggcagaagac ccaccgtttc
      841 atcgttcgac ctccgcgaca ccccggggcc gcgtcagagc tcctcgaccg tagtccacgt
      901 ctcgtcgggg tcgtacagct ccatcgcgcg gaactgcgcc tcggtctcgg gtccgaggtc
      961 cttctcgtac accacgcccg tgtgactgac gatgaacgtc atgatccctg tgacgccgta
     1021 gtcggcgggc gcggcgacga gcgcgaaccc gccgatcatc acgtccttga tccggaagtc
     1081 catcgccccg agcggtgcgc tcggtccctg gcccttgagg atcttgaaat agtagccgtg
     1141 atacggctcg atccggtccg tgtagccctc ggcgatgacc cgcgcgatcc cctcgccgac
     1201 cggtccggcc cacgtgccgt cgggctcctg ccacgccaga ccgtcgtgtc ttccgggcgt
     1261 gctgatgatg cgttgcgcgt actggtgcag gttcgatccg tcatgcttct ccgacgcgta
     1321 ctcgtgctgc gcctcggcgt acccgcggca gatgtcgatg gcgtcgagct cgttctggcc
     1381 gatgcggcga ttgaggattt cctcccgccc ggcctcggaa tcgaagcgcc agccgccacc
     1441 ctcctcgacg agcggaatcg gcagcggcca gtcgtcggga ccgaccgaca ggacggcgag
     1501 cgtcgagctt tccgggtcgc gctcgacccg catctgcgtt cgggcctcgg cggcgaacgc
     1561 ctgggcctgg ttccgatcct gcacccgatc ctcggtgacg acgagatcga tgccctccgg
     1621 gccgaggatg ttcgcgagcg ccgagacgtc gaaccgctcg gcggccgaga cgagagcctc
     1681 cgccgcctgc tcgggggtgg cgaatctctc ctggcgcgac ggcccggacg agcatccgat
     1741 cgtcagggcc agtcccaggc acagagcggt gctccgggga cgggtcatcg ccggcctccc
     1801 cgacccccgc cgcgcgtccc gcccgaacgg gacatgctcg acgacccgcg ggagctgctg
     1861 gcgcgcgcgc ccgagccgct gtagccccgt gagccgccac cgaagccgct gtgggcccgc
     1921 gagccggtgc tctgggggat actgcgattg ccgatcttgt caccgcccgc acctccggcg
     1981 cggctcgacg cccccggccc gcccgcgcga ttcgatgcgc tcgacccgcc cgcgcggttc
     2041 gaggcccccg gcccggccgc gcggttcgat gcgcccgcac cacccgctcg attcgacacg
     2101 ctcggctggc cggcccgcga ctcgccgcgc gccgacccgc ggtacttgtt ggcggtcgcc
     2161 ttgtcgccgt acggcgccgc cccgcggtgc gaggcgttgt gctgccactt gttgccgctc
     2221 ccgccgcggc cgccgcctcc gcggtcgccg ttccggttgt gctcgatccg gttgaagttg
     2281 ttgtcgacgt tgatgtcgat gtcgttcccg ccccagccgc atccgcagca cgagccgccc
     2341 cagaccgccg ctccccacat cactccgaag ctgaagctga cgaacgccgc gcccggcggg
     2401 tacggcggat agtagatcgg cgggtaggga tagagcggcg ggccatagac cacggtcggg
     2461 ctgtaagacg ggacgtagat cacctcgggg ttcgacgact cgacgatgat gacgtcgacg
     2521 ccgtcgacct gcttcgtttc gacgttctgc tgctcgttcg actcgagcgc gccgttgtcc
     2581 ttggccttct tccgcatccg ctggaccgcg tccatcacgt cggattgctg ggccaggaac
     2641 gcgttgccca ggtcggtcgt ccactggata tcctccgcca ggcgcttcac cacctcgggt
     2701 gttgcggcca tcgactggat gctcgggtcc cacggctgct tggccacttc gtcggccaac
     2761 gcctggtcct tcagctccgg atgcttggtc atccactgct ggagctggac gatctcgagc
     2821 gggtaggtcg acgcgacgag cacctgtgcc agcagcggat cggggtagag cgcgatcggc
     2881 gcgaccaacg agtcgagctg gtcggacggg atcttcgtca cctcgggcgc gggtgatgcg
     2941 acgggcgtcg gcgcgggcga cgcggccggc tgcggctcat cggcgagcag cggccccccg
     3001 ccgctcgaac cgagcaaggc cgcgcacgag acgatcgcga gcccgcgacg gaatcggagt
     3061 agattgggtt tcatggtcag cgaccctcgt ggagcgaatc gagaatagag cagacggcgc
     3121 ggccgtggca acaccaatcc cctgacgccg gtcgcgcgcc accgtgcccg gggttcaacc
     3181 cgccattctg ctcttcctgc cgccggagcc gcgccatgat cggtggcccg acgcggccgc
     3241 ttcagtacaa acgagctccg tcgtgcgagc gacggcggcg gtcatcgagg atccggcggt
     3301 actcgcggcg gtcctccgca tcgtcctcgt cgtcggcggc ccgttcgagc aacgcgtcga
     3361 aacgcggatc gtcgtgcaga ccgcgcaggt cgtcgtcgcc gagcagctga ccgaccgaga
     3421 tcgatccggc gtcgagcgcc ttgccgagcc agtccaacgc ttcgtccggg cgctcctcca
     3481 tcgcgtaggt gcagccgacg ttgtacgccg ccagcctccg ctgataaccg agctcgtact
     3541 gctcgaggaa cgcttcgcga gcctcgccga agcggcgcga gtgatgcagc ccccacccca
     3601 ggttgctcca cgcgcgaccc aggcccggtt gctccccgac cagctccgtg tagtcctcga
     3661 ccgccggggc ccatcgtcgc gcggagtagt ccgagcccca gttccaccag ctgcggaatc
     3721 gagcgagcga gagccgatca tgcaagcgtt cgatctccgc gaagcgcgcg gtctcgcgaa
     3781 tcccgtcgag atcggagtcg ttgcggaacc gctcctcgct gtcgaaaccg gcggccaccg
     3841 cgcgctcgag atgatcgagc gcctggcgct cgtcgccggc gagcgagtag gcgcaggcga
     3901 ggttgtacag cgcgttggcg ttgccgtcga gcatgcgcgc cgctcggtcg agtgcgtcga
     3961 tcgcccgatc ggactcgcgc attccgagca gcgccgaccc gacgtcgtac caggcccggc
     4021 cgtcggtcga gtcctgcgcg accaggtcgt cgtaccgatc gaccgcgccc tcgtaccgtc
     4081 cacccgacgc gcgatgcgcg ccgggcagct cgtcgatcat ccgccggaac gtcgcctcgc
     4141 gtcggatcgg gtcgaggtcg cgatcgctcg cgatcagctc ggggtcgtcg aagccggcgt
     4201 cgacggcgcg ctcgatcagc tcgacggcgc gctccgcctg gcccaacctc gcgtaaccgc
     4261 acgccgcgtt gtacaacgac accgcctcgc ggtagccctc gtcggccgcc gcgatgaaac
     4321 cggcggccgc ctcgtcccag cgttcttgcc ggtggagcgc cagcgcctcg cggaaacgcc
     4381 gttctccgtc gcccggccgg tcatgcgcca gcatgatcgc gccacgatcg tcgtcgtcga
     4441 tacgtaccgc ctgcgttgcc gcggacgggg aagcgatccg ctcgggcgtg gacgcctcgg
     4501 cccgctcggc ccactgggcg ctcgcgatcg aaaccacggc gaccacgctg agcgacgaca
     4561 ggacccatgc tccacgccgc cgcagaccgc gccgcgcgtc ggcgctcagg atcgagcgga
     4621 cccgagccgc cagttgattg cgttccgcga tgccgagcgt cgacgccggc gcgcgggcgg
     4681 ggagttgtcc ggcgatcgcg agcagctggc cggcgtaacg cgagggccgt tcaccgcagg
     4741 ccagcacgac gtcgtccgcg gcgcgctcgc agtcggcccg ggccgcacgt tcgacccacc
     4801 atgccagcgg gtggaaccac cacagcgcga ccgacagccg gccgagcaac aaccacagac
     4861 cgtccgcgcg acgacggtgc gccagctcgt gcagcagcac ggcatcgagc cgcccgcggt
     4921 cccagtcgca cgcggtgcga ggcagcagca gggtcggatc gacgaagccc cacaagaccg
     4981 gcagccgcag cgcgtcggtc gtcagcacgc gtggagggcg cgcgagaccg acctgccgcg
     5041 cgcaacgctc gagcgcttgc cgcgcgggtc cgtgcacgag cggcgtggcc tcgatcgcga
     5101 tccggttgac gcggaaccag ccgacgccga gacgcagcag cacgaacgcg gcccccacgc
     5161 cccacagcca gggcacccac gcgagtgggg cgggcttcga gcgtggcgcg acctcgcgcg
     5221 gtgcggccac cccggccgtc gagacggccg cggtcgcggg ctcgtcgacg accggctcgc
     5281 ggcggtagga ggcggcgacg cgcggttcgg ccggctcgtc gaggtcggcg ggaggatcga
     5341 acgcgggact cgtcgcggtc gcaccggcgg cgagcgccga tcgcggaccc gcgagcggca
     5401 gctcccaggc cggcagcacg acggcgagcg cgggcaggac gagcaacgcc accagacccg
     5461 acgctcgcaa gagatgccgg gccgcggccg acgatctgcc gacgagaccg ccgaggagga
     5521 cggccagcag caccagcgtg gacttgatcg cgagttcgag catcatctcc cttccctccg
     5581 tgcctggcgg atcagccgcg acaatcgttc gatctcttcc tggttcagtt ccgtgccctg
     5641 ttcctcgagc agcgcggtga ccgcgtcggc cgccgaaccg tcgaagaagg tgtcgagcac
     5701 gtggacgagc gcagaccgcc gggcctcggc ccgggtgcgt gtcggcaggt agacgtagcg
     5761 gttgccctcg tggcggtgcc ggaggtggcc tttggcttcg aggatgttga gcgtgctgcg
     5821 aacggccgag tagctcggcg gctgctcgat cgcctcgagc acctcggtca ccgtcgcctc
     5881 tccggcgcgg tagatgatgt ccatcacctc gcgttcgcgg cggctcagcc gggacccttc
     5941 ttgctgtttt tttcgcatat gctgaatttt tagcacacgc ccgatcggtc gtcaaggttt
     6001 tctacgtccg aacgccgcgg cggttgcgcg ctcgccggtg taacccggcg gcggcgcccg
     6061 tggtagtaag gggtgcaggc tgccttcgag cgggatgacg gtgacgacgg tcaacgacag
     6121 cgtcttgatg acggacgttc gcgacggccg agccgccgcg ctcggcacgc tgttcgagcg
     6181 gcatcacgcc aggctttacc gcttctgcct gcggatgacc ggcaaccggt cggtcagcga
     6241 ggacctggtg caggagatct tcatgcgcat cttgcggcat cgagcgacgt ttcggccggg
     6301 cacggcgttc gtgccgtgga tgtaccgcat cgcgcgcaac gcctgcatcg atcacctgcg
     6361 gcgcggcgcg gccggcggcg aggcgctcga agaagaagag acgctggtcg cgggcgggcc
     6421 gtcggtcgag gaaaccgtcg agcgcggtcg ggcggcccag ctgatgcgac gcgcgctgct
     6481 gcggctgccg gtcgagcggc gcgcggtact gttgatgagc cgctacgagt tcaagaccta
     6541 cgaggagatc gcccgcacgc tcgactgctc ggtcagcgcg gtcaagatcc gcgcctaccg
     6601 ggcgatcaag caactacgcg aagtctactg cgatctggca cgggaggctg cgccatgaac
     6661 tgcgacgaga caaccggacg cgtgtcggac cgtctcaagc ggctgctcga cccgcggtcc
     6721 gaggccgacc tcgaacggca cttgtcccgg tgcgacgact gccgcgcgga ggcggaggcg
     6781 atcgagcggg tgtggaacga gatgggacgg ctcgacgacg aggtgccgag cgaccggatg
     6841 cgctcgcgat tccacgacgc gctcgcggcc tacgagcggc gcgacgcggg ccccgccccc
     6901 gcgcgcgggt cgggacgccg cctcggcgcg ctgtgggcgc ggctaccggg cgcacaactg
     6961 gcatcgatgg ccgccgcgct gtcggtcggg atgctcgtcg gcggcttttg gctcgcgccg
     7021 gggacgggcg agatcgaggc gttgcggttc gagctgaggt cgatggagcg cgcgatctcg
     7081 ctgacgctgc tcgagcacgg ctcggcgagc gagcggctgc gcggcgtgca gttcagcgcg
     7141 cgggtcgagc cggactcccg cgtcgtcgag gcgttgctcg ataccgtcgc cgccgacccc
     7201 aacgtcagcg ttcggctggc ggccgtcgag gcgctcgccc cgctgctcga tcggcggccg
     7261 atcgccgagc ggttggtcga gacgatggcc gagcagagct cgccgctgct ccaggtcacc
     7321 ctggccgagg tcctgctcga tgccggcatc gaaggctcgc ggcccgccgt cctgcggctg
     7381 atcgagcacg aaggcaccga ccccgccgtg cgcgagcacc tgcgtggagt gatgcgcaac
     7441 tccggttgag ccggacgagg agagcgagat gaagaaagtg acagcgaccc tcatcgcggc
     7501 cctggcgctc ggcgcgacgg ccggcgccgg cgagctggtc gagcggagga cgattcgcga
     7561 cgcgatggcc gtcgcgggcg acgggcgcac gggcgagctg gtcgtcgaca acgtgttcgg
     7621 ctcgatacgg gtcgaaacgc acgaccgtcc gctgatcgag tacgtcgcgc acgagacgat
     7681 cgaggcgcgc gatcgtgacg ccgtcgaccg ggcccgcgag gaggtccgtc tcgacgtccg
     7741 tcgagaggga gacagctaca cgctgttcgt cgacggaccg ttccgctgcg acgacgaccg
     7801 gcgctgtcat tgcggccgcg agacggacta cgtcgtcgtc tacgacttcg agatccgcgt
     7861 tccccggcgg atcgacgtcg acctgcgcac ggtcaacggc ggcgagatcg aggtgaccgg
     7921 cgtccgcggc gactaccgcg tggcgaacgt gaacggcggc gtgcgattgc tccacgtcga
     7981 cggctcggga cgagcgaaca cggtcaacgg cccgatccgc gtcgtgttcg acggcaaccc
     8041 gcgggcggaa tcggagttcc gcaccgtcaa cggcgatgtc gacctcgagt tccagccgga
     8101 cctgtcggcc gacgtccggg ccaagacctt caacggagag atctggaccg acttcgatgc
     8161 cgaaccgcta ccgctcgacg cgccgcagac cgcccggcgg gacggcaaga ccgtgatccg
     8221 taccgatcgc tgggccgcgc tgcgcatcgc cggcggcggc ccgctgctga cgttcgagac
     8281 gctcaacgga gacatcctga tacgcaacgc gcttcgctga cgcgcgacga ggtgctcatg
     8341 tttcgccgag tcatcctgcc gacgatcgtt ctcatcccga tcgtgatcgc gacgccgggg
     8401 gccgcggcgc agcagggcga gggccagcgg ctggtcgtgc cgatcagcga cccgagccga
     8461 ccgatcgtgc tgcgcgcctc gctggtgtcg ggcggcatcg tggtcacgcc gcacgacggc
     8521 cgcgaggtga tcgtcatcgc ccggccgcgc gacgccgagc tcgatgcgcg gcgggccgac
     8581 ggcatgcggc tgattcccaa cacttcggtc ggcgtgaccg ccgaggagga ggccaatcgg
     8641 atcgacgtcc aagccgactg gagctcgcgc gggatcgacc tcgagatcca ggttccggta
     8701 cgcacctcgg ccgagctgtc gacggtcaac tcgggggaca tcgtcgtccg cggcctcacc
     8761 ggcgacctcg agctacagaa cacgaacggc tcgatcgtcg ccgaggacat cgacgggtcg
     8821 gtcgtggcga acacgaccaa cggcacggtg cgcgtccgat tcgatcgggt caaacccgac
     8881 accgacatgg cgttcaacag cttcaacggc gacgtcgacc tgaccttccc cgcctcgctc
     8941 gaggccgagt tcaggatcag cgtcggacgc ggggagatcc gcaccgattt cgaggccgtg
     9001 cagcggccgg cttctcccaa ggtcgagcag gacggcagc
//
LOCUS       DASPGN010000213         8547 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_973146, whole genome shotgun sequence.
ACCESSION   DASPGN010000213 DASPGN010000000
VERSION     DASPGN010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8547
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973146"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VD788_06055"
     CDS             complement(<1..848)
                     /locus_tag="VD788_06055"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXV75867.1"
                     /translation="MDGNQAFICDALRTPIGRYAGSLSAVRADDLAAAPIRAIVERRS
                     DADWDALDDVILGCANQAGEDNRNVARMALLLAGLSDAVPGVTVNRLCGSGMDAVAQA
                     ARAIRTGEASFVIAGGVESMSRAPFVLPKSPAAFGRGLSLEDTTIGWRFVNPEMKRRY
                     GVDSMPETAENVAREYGVARDAQDAFAWRSQTRARAAIESGRMAEEIVPVRVSVRKGD
                     PRVVDRDEHPRPDSSLDTLASLPPLFGDGGTVTAGNASGVNDGACALVLASERAAATH
                     GLRPRAR"
     gene            1119..1979
                     /locus_tag="VD788_06060"
     CDS             1119..1979
                     /locus_tag="VD788_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75868.1"
                     /translation="METNGRGSAAWFGPAGLTKAVVLLALATPIGTVPAGENSWVTKG
                     GGAPAHAIVTSEDAETFDLSELADGETRVFGVGDAQVSVRREGETATIYRAATGERPE
                     LDITCRLADDSCKIVTPADHPDQVAILISRERDCVNGEGDCEPAALLTDLAHDGAGHA
                     VLIHTRVECDGDADCATAELHQHHAGEPAVIRLETLGIGAGDSEVFVLGEGGSAAGSG
                     LARVIVERAGDETVLRCPEGDTTMRVAREEAEAFYLCPKHSRQLERVEHAPGMPRLEL
                     IRPDGETAEH"
     gene            2024..2806
                     /locus_tag="VD788_06065"
     CDS             2024..2806
                     /locus_tag="VD788_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HXV75869.1"
                     /translation="MNEFAARPLALVGFPTDEHSSYLRGSADGPARIRSALRSGASNL
                     VAEDGTDLTDRLDDLGDRHGSIEDAVRAVLTAGRRPLALGGDHSITFPIVTALAGAVG
                     PLDLLQFDAHPDLYDEYGGDRLSHASPFARILERGLARRLVQVGVRTMNEAQRRQVER
                     FGVEVVEMRSWRVGLPLAFDGPLYLSVDLDVLDPAYAPGVSHLEPGGATTRQLIDAIR
                     EVRGAIVGADVVELNPRRDPSDLTPAVAAKLVKELAARMLRR"
     gene            complement(2830..4089)
                     /locus_tag="VD788_06070"
     CDS             complement(2830..4089)
                     /locus_tag="VD788_06070"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV75870.1"
                     /translation="MPRPAAREVGRYHLGDLLGRGGIAEVYHAVDPRLHRRVAIKTIR
                     VGRAARPESLARLAREMKLVLALEHPNILPVYEVGQSDGEPYLVMPLLGGGTLTERIA
                     RGVGERDALRWVTRLADALDVAHALGILHGDVKPSNVLFDRAGRPLLADFGLAPLVAA
                     AGPAAGGSGYTAPELARGREPSPASDLYSLAALTRELLSSPAPAQAVIARALAEDPAA
                     RHRSCGEFAGALAAALEPPAAAGAPLAPSAVPDLGADGPRSVTAAWLEGAGPEGRDRH
                     RRTWTMLLVLLFAMLGALLGARSLLDRSNGARTGTGRSVRSVPEAAVRAHLDGNHDHA
                     LALAVGAIEALPRRSNEARARLLAFVAKEQVELGLWRDAVTTCETIAGLGVVASDEAA
                     RCADEIARDRLHDLEATRRLLEAARER"
     gene            4276..4863
                     /locus_tag="VD788_06075"
     CDS             4276..4863
                     /locus_tag="VD788_06075"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HXV75871.1"
                     /translation="MSRRGEGSVARILLATIALAAALPAAGQQGGDAQGFVKAISPGE
                     HHAHFKKMVGKWNAAVRSWPVPGGEPTEVSGATEYELVLGGRYLQQKMTAQMMGMPYE
                     GLGLSGYDNTAGKHTLLWVDSMSTWMTYGEGDCSDDHKTQTIRAEVKNPMGGPTMRAK
                     LVTRIESDDKHVFEYYIVGDDGSETKVLEVVYTRA"
     gene            complement(4860..6296)
                     /locus_tag="VD788_06080"
     CDS             complement(4860..6296)
                     /locus_tag="VD788_06080"
                     /inference="COORDINATES: protein motif:HMM:NF023407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HXV75872.1"
                     /translation="MRTTVTLSLISHTNVGKTTLARTLLRRDVGEVIDREHVTERAES
                     FTLIETERAVLRLWDTPGFGNSANLLRLLRRHRSPIGWLLHQTWDRVVNRPLYCSQQA
                     AANVKQEADVVLYLVNAAEDPTDAGYVTLELELLGWIGRPVVLLLNQIGTDGHALETR
                     WRELARQSSVVRDVLSLDAFTRCWVEEEILLRRVGRVLEGDKRPAMDELVRAWRERSL
                     ETFRASVARLASYLVEAAGERESEATGGGSSGLRSIAGALKFSAIDKRRAMRLLDERL
                     DRATERLLDELIAAHGLEGASARRIERRVHDFEVRGDGIPIDERSGAVAGAVISGALT
                     GLAADALSGGLTLGGGMIAGGILGALGGAALGRGYRWAGGSDAPSVGWSSEFLARLTR
                     QAVLRYLAVAHFGRGRGRFRDVEYPAHWSEAVDGALAPLRKRLDAVWERARRPDADGA
                     RVELTGLLGSAIESVLAAAYPAAERDRA"
     gene            complement(6296..7888)
                     /locus_tag="VD788_06085"
     CDS             complement(6296..7888)
                     /locus_tag="VD788_06085"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HXV75873.1"
                     /translation="MRDPGLGERRAQVILLVRAFEEADPEGLILPLRERNKATRRALM
                     VTGLSSDTADSAAAWRIRHDEAIMRRARLLFDLLIRRTPSLERIVRISQLGASTGPVV
                     VGVAFLLGVLTNALGGKQINLLSFPLIGLLGWNLLAYLAMLSWPIVRRGRGVVDLAER
                     IGGYLFRGAVWRRLRSSKVAEGGVSPETRIIGKAVMRFSAMWHRLAGRVLASRVKRIL
                     HLGAIATIAGVVVGMYVRGFAFEYRVSWESTWLDTRQMQSLLALVLGPASLVLGLELP
                     DVAPLRGPAGSGDAAPWIHLYAMTALLVVGVPRALMALYQGWNGYRLSRRLPLDVNEP
                     YYRRLFTAWRGATRHVRIVPYSYTPKPGALASLKTLLFDYFGARADLHVSDPLAYGSE
                     ASEVTVVRQDDEPAVAKRETCLVLVFNGAQLPENDVHGEFLEELTARLDTRSSRVLVV
                     LDLGPYRSRVADEQRVRERTANWLAVVAAAGLNALPLDLERSVSPAGRAGELTKEGAA
                     SDLLGAVHASLWPGPEAVETTG"
     gene            8011..8406
                     /locus_tag="VD788_06090"
     CDS             8011..8406
                     /locus_tag="VD788_06090"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXV75874.1"
                     /translation="MAMQITIGSGGRVDAVYKGRTIRTAQDGTEPAPFDLFLASIGTC
                     TGYYVARFCESRGIPTDGIRIEQTRVIDEETRMVERVRVEINLPDDFPERYREALLRS
                     AALCSVKKHLERPPRVEVETRIEQTAGRG"
BASE COUNT         1312 a         3105 c         3010 g         1120 t
ORIGIN      
        1 cgcgcgcgcg gccgcagacc gtgcgtcgcc gccgcccgct cgctggcgag caccagcgca
       61 cacgcgccgt cgttgacccc cgacgcgttg ccggcggtca ccgtcccgcc gtcgccgaac
      121 agcggcggga gcgacgccag cgtgtcgagc gagctgtccg gccgcgggtg ctcgtcgcgg
      181 tcgacgaccc gtgggtcgcc cttccgcacg ctcacgcgga ccggcacgat ctcctcggcc
      241 atccgtccgg attcgatcgc cgcgcgcgcg cgcgtttggg agcgccaggc gaaggcgtcc
      301 tgcgcgtcac gggcgacccc gtactcgcgc gccacgttct ccgcggtctc gggcatcgag
      361 tccacgccgt agcgccgttt catctcgggg ttgacgaacc gccagccgat cgtcgtgtcc
      421 tcgagcgaca gcccgcggcc gaaggccgcg ggcgacttcg gcagcacgaa cggagcccgg
      481 ctcatgctct ccacgccgcc ggcgatcacg aacgacgcct cgccggtgcg gatcgcccgc
      541 gccgcctgcg ccaccgcgtc cattcccgac ccgcacaagc ggttgaccgt gacgcccgga
      601 acggcgtcgg acaggccggc caacaacagc gccatccgcg ccacgttgcg attgtcctcg
      661 ccggcctggt tggcgcagcc gagtatcacg tcgtcgagcg cgtcccagtc ggcgtcgctc
      721 cgccgctcga cgatcgcgcg gatcggcgcg gcggccaggt cgtccgcccg gaccgccgac
      781 aagctccccg cgtagcgtcc gatcggggtt cgcagcgcat cgcaaatgaa cgcctggttg
      841 ccgtccatcg tcgctccttt cgctttcgat ccgtgccgga ccgctccgga tcgtagcaga
      901 tcgggagtgg cccggagctc gacccaattc gggcgggtct gccccaaatg agacggctcg
      961 cccgacgcga gtcgaccgcg accgggcatc ggaggccggc cacggcgtcg cgaaccacgc
     1021 cgcgtctctg gcacgggtcg tgcacttccc gagcgcgacg cccggccggc gggcgtcgtg
     1081 gaacgagaac gcatcgatcg acgatcgagg aggaaccaat ggaaaccaac ggaagaggat
     1141 cggccgcctg gttcgggccg gccggtttga cgaaggcggt cgtgctgctc gcgctggcca
     1201 cgccgatcgg cacggtcccg gccggcgaga actcgtgggt gacgaagggc ggcggggcgc
     1261 cggcccacgc gatcgtcacg agcgaagacg ccgagacctt cgatctgtcc gagctcgcgg
     1321 acggcgagac tcgcgtcttc ggcgtcggcg acgcgcaggt cagcgtacgg cgcgagggcg
     1381 agaccgcgac gatctaccgc gccgccaccg gcgaacggcc cgagctcgac atcacctgcc
     1441 ggttggccga cgacagctgc aagatcgtga cgccggccga ccaccccgat caggtcgcga
     1501 tcctgatcag ccgcgagcgc gactgcgtga acggcgaggg cgactgcgag cccgccgcgc
     1561 tgttgaccga cctcgcgcat gacggggccg gccacgcggt gctgatccac acgcgtgtcg
     1621 agtgcgacgg cgacgccgac tgcgccacgg cggaactgca tcagcaccat gccggggaac
     1681 ccgcggtgat ccgtctcgaa acgctcggga tcggtgccgg tgacagcgag gtcttcgtcc
     1741 tcggcgaggg cggaagcgct gccggtagcg gcctcgcccg cgtgatcgtc gagcgcgccg
     1801 gcgacgagac ggtgctgcgt tgcccggaag gcgacaccac gatgcgggtc gcccgcgagg
     1861 aagccgaggc gttctacctc tgcccgaagc actcgcggca gctcgagcgg gtcgaacacg
     1921 cgccgggcat gccgcgactc gagctgatcc gaccggacgg ggaaacggcg gagcactgag
     1981 gctgttcgtg cgacccgccc ggcgcgtcgg ctaagctgtc acggtgaacg agttcgccgc
     2041 gcggccactc gcgctcgtcg ggttcccgac cgatgagcac tcgtcctacc tgcgcgggtc
     2101 cgccgacgga cccgcgcgca tccgttccgc gctccgttcg ggtgcgagca acctcgtcgc
     2161 ggaggacgga accgatctga ccgatcggct cgacgacctc ggtgaccgcc acggctcgat
     2221 cgaggacgcc gtccgggcgg tgctcaccgc cgggcggcgt ccgctcgcgc tcggcggcga
     2281 tcactcgatc acctttccga tcgtgacggc gctggccggc gcggtgggtc cgctcgacct
     2341 gctgcagttc gatgctcacc ccgacctgta cgacgaatac ggcggcgacc ggctgtcgca
     2401 cgcgtcgccg ttcgcgcgca tcctcgaacg aggattggcc cgccgcttgg tccaggtcgg
     2461 cgttcgtacg atgaacgaag cgcagcgccg gcaggtcgaa cggttcggcg tcgaggtggt
     2521 cgagatgcgc tcgtggcgcg tcgggctgcc gttggctttc gacgggccgc tgtacctgtc
     2581 ggtcgacctc gacgtgctcg atccggcgta cgcgccgggg gtctcccatc tcgagcccgg
     2641 cggagcgacg acccgccagc tgatcgacgc gatccgcgag gtgcgcggtg cgatcgtcgg
     2701 ggccgacgtc gtcgagctga acccgcggcg ggacccgagc gatctcacgc cggccgtcgc
     2761 ggcgaagctg gtcaaggagc tggcggcgcg catgctccgc cgctgatccg gttcgtgacc
     2821 cgagctcggt tagcgctcgc gagccgcctc gagcaggcgt cgcgtggcct ccaggtcgtg
     2881 cagtcgatcg cgggcgatct cgtcggcgca ccgtgcggcc tcgtcggagg cgacgacgcc
     2941 gagcccggcg atcgtctcgc aggtcgtcac cgcgtcgcgc cagagcccga gctcgacctg
     3001 ctccttcgcg acgaacgcca acagtcgcgc gcgggcttcg ttcgaccgcc gcggcaacgc
     3061 ctcgatcgcg ccgaccgcca gcgcgagcgc gtggtcgtgg ttgccgtcga ggtgtgcgcg
     3121 gaccgcggcc tcggggacgg aacgaacgga ccgaccggtg ccggtccgcg caccgttcga
     3181 gcggtcgagc agcgagcgtg ccccgagcag tgcccccaac atcgcgaaca gcagcacgag
     3241 cagcatggtc cacgtgcggc gatgacggtc ccgcccctcg gggccggcgc cctcgagcca
     3301 tgccgcggtc accgaccggg ggccgtccgc gccgagatcc gggacggcgg acggcgcgag
     3361 cggtgcaccg gctgcggccg gcggctcgag cgccgcggcg agcgcgccgg cgaactcgcc
     3421 gcacgatcga tgacgcgccg ccgggtcttc ggccaatgcc cgcgcgatca cggcctgcgc
     3481 gggagcaggt gacgacagca gctcgcgggt cagcgccgcg agcgagtaca ggtcgctcgc
     3541 cggcgacggc tcccgaccgc gggccaactc gggagccgtg tagcccgacc cgccagccgc
     3601 cggcccagcg gcggcgacga gcggcgccag accgaagtcg gccagcagcg gtcgtccggc
     3661 gcgatcgaac agcacgttcg acggcttgac gtcgccgtgc aggatgccga gcgcgtgcgc
     3721 gacgtcgagc gcatcggcca ggcgcgtcac ccaccggagc gcgtcccgct cgccgacgcc
     3781 gcgggcgatg cgctcggtca gcgtccctcc gccgagaagc ggcatcacga ggtacggctc
     3841 gccgtccgac tgaccgacct cgtagaccgg caggatgttg gggtgttcga gcgccaggac
     3901 gagcttcatc tcgcgtgcca gccgggccag cgactccggt cgcgccgccc ggccgacccg
     3961 gatcgtcttg atcgcgaccc ggcgatggag acgggggtcg accgcgtgat acacctcggc
     4021 gatcccgccc cggccgagca gatcccccaa gtgatagcgc ccgacctcgc gggctgccgg
     4081 cctcggcaag ttcggctctc ctcccgggcg ggcggcatcg ccacgaacgc ccccgaggcg
     4141 cgccggccgc cgagatcgaa tctagggctg ccgtcatcga atgccaggac ggggcggccg
     4201 gcccctttcg gcgggcgctc cgatccgcta gattgggccg gtgcgggagg gcaccggttc
     4261 ggggaggagg ggctgatgtc tcgacgagga gaggggtcgg tcgcgaggat cctgctggcg
     4321 acgatcgcgc tggccgcggc actgccggcc gccggacagc agggcggcga cgcccagggg
     4381 ttcgtcaagg cgatctcgcc cggcgagcac cacgcacact tcaagaagat ggtcggcaag
     4441 tggaacgccg cggtccgcag ctggccggtg cccggcggcg agccgaccga ggtctcgggc
     4501 gcgaccgagt acgagctcgt gctgggtggt cgctacctcc aacagaagat gaccgcccag
     4561 atgatgggca tgccctacga gggactcggg ctgtcgggtt acgacaacac cgccggcaag
     4621 cacacgctgt tgtgggtcga ctcgatgagc acctggatga cctacggcga gggcgactgc
     4681 agcgacgacc acaagaccca gacgatccgc gccgaggtga agaacccgat gggcgggccg
     4741 acgatgaggg ccaagctggt cacccgcatc gaaagcgacg acaagcacgt cttcgaatac
     4801 tacatcgtcg gtgacgacgg atcggagacg aaggtactcg aggtcgtcta cacccgcgcc
     4861 taggcccggt cccgctcggc ggccggataa gccgccgcca gcaccgactc gatcgcgctg
     4921 cccagcagcc ccgtcagctc gacccgcgct ccgtccgcgt cggggcgccg ggcgcgctcc
     4981 caaaccgcat cgaggcgctt gcgcagcgga gcgagcgcac cgtcgacggc ctcgctccag
     5041 tgcgcggggt actcgacgtc gcggaaccgg ccgcgaccgc ggccgaagtg agccaccgcc
     5101 agataacgca ggacggcctg cctcgtcagt cgggcgagga actcgctcga ccagccgacc
     5161 gacggggcat cgctcccacc ggcccagcga taaccgcggc cgagcgccgc gccgcccagc
     5221 gccccgagga tcccgccggc gatcatcccg ccgcccagcg tcaagccccc cgacagcgcg
     5281 tccgccgcga gaccggtcaa cgcgccgctg atgaccgcgc ccgcgacggc gccgctccgc
     5341 tcgtcgatcg ggatgccgtc gccgcgcacc tcgaagtcgt ggaccctgcg ctcgatgcgg
     5401 cgagcgctcg ctccctcgag accgtgcgcc gcgatcagct cgtcgagcag ccgttcggtc
     5461 gcgcggtcga ggcgctcgtc gagcagccgc atcgcccgtc gtttgtcgat cgccgagaat
     5521 ttgagcgcgc cggcgatcga gcgcagcccg gacgaaccgc ctcccgtcgc ctcggactcg
     5581 cgttcgcccg cggcctcgac gaggtaggac gcgagacgtg cgacgctcgc gcgaaacgtc
     5641 tcgaggctcc gttcgcgcca ggctcgcacc agctcgtcca tcgccggccg cttgtcgcct
     5701 tcgagcacgc ggccgacccg ccgcagcaaa atctcctctt cgacccagca gcgggtgaac
     5761 gcgtcgaggg acagcacgtc gcgaacgacc gacgactgcc gcgccagctc gcgccagcgc
     5821 gtctcgagcg catgcccgtc ggtgccgatc tgattgagca gcaacaccac cggacggccg
     5881 atccagccga gcagctcgag ctcgagcgtg acgtagcccg cgtcggtcgg gtcctccgcg
     5941 gcgttgacca ggtacaacac cacgtcggct tcctgcttga cgttggccgc ggcctgctgg
     6001 ctgcagtaga gcggccggtt gacgacgcga tcccacgtct ggtgcagcag ccagccgatc
     6061 ggactgcggt ggcggcggag cagccgcagc aggttcgccg agttgccgaa gcccggcgta
     6121 tcccacaggc gcagcacggc gcgctcggtt tcgatcaagg tgaacgactc ggcgcgctcg
     6181 gtcacgtgct cgcgatcgat cacctcgccg acgtcccggc gcagcaacgt ccgcgcgagc
     6241 gtggtcttgc cgacgttggt gtgcgagatc aggctcaacg tgacggtcgt ccgcatcagc
     6301 cggtggtctc gaccgcctcc ggaccgggcc acagcgacgc gtggaccgcg ccgagcaggt
     6361 cgctcgccgc accctccttg gtcagctcgc ccgcccggcc cgccggcgac acgctgcgct
     6421 cgagatcgag cggcaacgcg ttgagccccg ccgcggcgac gaccgccaac cagtttgccg
     6481 tgcgctcgcg cacgcgctgc tcgtcagcga cgcgcgagcg gtagggcccc aggtcgagca
     6541 cgaccaacac ccgcgagctg cgcgtgtcga gccgtgccgt cagctcttcg aggaactcgc
     6601 cgtgcacgtc gttttcgggt agctgcgccc cgttgaatac gaggacgagg caggtctccc
     6661 gtttggcaac cgccggttcg tcgtcctggc gaacgacggt gacctcgctc gcctcgctgc
     6721 cgtaggccaa cgggtcgctg acgtgcagat cggcgcgcgc gccgaaatag tcgaacaaca
     6781 acgtcttgag cgaggcgagc gcgcccggct tcggcgtgta gctgtagggc acgatccgca
     6841 cgtggcgcgt cgcgccgcgc caggcggtga acagccgccg gtagtacggt tcgttgacgt
     6901 cgagcggcag ccggcgtgag agtcggtagc cgttccagcc ttgatagagg gccatcagcg
     6961 cgcgcggcac accgaccacg agcagcgcgg tcatcgcgta gaggtgaatc cacggcgcgg
     7021 cgtctccgct gcccgccggt ccgcgaagcg gggcgacgtc gggcagctcg aggccgagca
     7081 cgagggacgc cggtccgagg acgagcgcga gcaacgactg catctgccgc gtgtcgagcc
     7141 acgtgctctc ccagctgacg cggtactcga aggcgaaccc gcgcacgtac atgccgacga
     7201 cgacaccggc gatcgtcgcg atcgcgccga gatgcaggat ccgcttgacc cgcgatgcca
     7261 gcacgcgccc ggcgagccga tgccacatcg cgctgaagcg catcaccgcc ttgccgatga
     7321 tgcgcgtttc gggcgagacg ccgccctcgg ccaccttcga gctgcgcaac cgccgccaga
     7381 ccgcgccgcg gaacaggtaa ccgccgattc gctcggcgag gtcgacgact ccgcgaccgc
     7441 gccggacgat cggccaggac agcatcgcca ggtaggcgag caggttccag ccgagcagac
     7501 cgatcaacgg aaaggacaac aggttgatct gcttgccgcc gagcgcgttg gtcagcacgc
     7561 cgagcaggaa cgccacgccg acgaccaccg gccccgtgct ggcgccgagc tgcgagatgc
     7621 ggacgatccg ctcgagcgac ggcgtgcgcc ggatcaacag gtcgaacaac aaccgggcgc
     7681 gccgcatgat cgcctcgtcg tggcggatgc gccaggcggc ggcgctgtcg gcggtgtcac
     7741 tcgacaggcc ggtgaccatc agcgccctgc gggtcgcctt gttccgctcg cgcagcggga
     7801 ggatcagccc ctccgggtcg gcttcctcga acgcccggac caagaggatg acctgcgctc
     7861 gccgttcgcc cagaccggga tcgcgcacgg cccccattgt atcgaaggcg accgaaacgg
     7921 tcccgaggcg cacggtgcgc gcgtgacccc cgggcgcgag aatgcatctc ctgaggtgtg
     7981 ggcgagccgg atcgacgcgg aggacgcaag atggcgatgc aaatcacgat cggttcgggt
     8041 ggtcgcgtcg acgccgtgta caaggggcga acgatccgca cggcgcagga cggcaccgaa
     8101 cccgctccgt tcgacctgtt cctggcctcg atcggaacct gcaccggcta ctacgtcgcg
     8161 cgcttctgcg aaagccgcgg catcccgacc gacggcattc ggatcgagca gacccgggtc
     8221 atcgacgagg agacccggat ggtcgagcgg gtgcgggtcg agatcaacct ccccgacgac
     8281 tttcccgaac gctatcgcga ggcgctgctg cgatcggccg cgctgtgctc ggtcaagaag
     8341 cacctcgagc ggccgccacg ggtcgaggtc gaaacacgca tcgagcaaac ggccggccgg
     8401 ggctaggagc ctgtccgagt attgcctacg gccgcgttcc ggacgccgcg gggccggatg
     8461 caaggcgccg cgctgcagac agatgcgacg gcatcggcca ggcgcggcaa cgccgcagac
     8521 ggccccgcgc cgccggaacc cgtcggg
//
LOCUS       DASPGN010000214        10020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_739421, whole genome shotgun sequence.
ACCESSION   DASPGN010000214 DASPGN010000000
VERSION     DASPGN010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739421"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VD788_06095"
     CDS             complement(<1..1098)
                     /locus_tag="VD788_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXV75875.1"
                     /translation="MKITTESIVRDYRVMFQSRQASLLGRKEVFSGKAKFGIFGDGKE
                     AAQVAMARSFRDGDFRAGYYRDQTFMLAAGLLTLDEFFAQLYAHADVRADPASAGRQM
                     TAHFATRMLDDDGAFRVLAESKNSSADLSPTGSQMPRLVGLGYASKLFRQIAELSAWP
                     RLSNRGNEIAFGTIGNASCAEGLFWESVNAIGVLQVPVIISIWDDHYGISVPNEFQIC
                     RNDISAVLSGFQRENGDAPGFDVHRVPGWDYAALCETYLGAAEVARRDHVPSIIHVTE
                     MTQPQGHSTSGSHERYKDESRLAWEERHDCIARMREWMLSQHIVDRDELDHIEQEDKE
                     VANAARRRAWDAYARELRAERDELVATLSWGG"
     gene            complement(1221..1817)
                     /locus_tag="VD788_06100"
     CDS             complement(1221..1817)
                     /locus_tag="VD788_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXV75876.1"
                     /translation="MNSVRGPNTLRERISAAAQELFLAEGVDGVSMRKVADRVGVTAP
                     AIYRHFKDKDELLSEIINVGLMTLQRYLEPAFRAERPFDRLQRMIEYYLDFALEQPRY
                     FDFAFLVPSHGIELTEELARHNRSTFMFAVQQVHLCMEQGVFVKSDPLETAIMVWAEA
                     HGLVTLFRLERFGQDPEQFRGVFRRTIQRLLDGLKTGA"
     gene            2091..4082
                     /gene="shc"
                     /locus_tag="VD788_06105"
     CDS             2091..4082
                     /gene="shc"
                     /locus_tag="VD788_06105"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HXV75877.1"
                     /translation="MSRQLTTAVPVTETPDDLRFRAREAIESSQRHLLAIQNEDGHWC
                     GELEGDTILESEYILAMHFLGRTHEDRVRKAANYLRDKQLPGGGWGVYPGGPAEVSAS
                     TKAYFVLKLVGDDPDAPHMSAARRTIRALGGLDACNSFTKIYLAVFGQLDWDDCPSVP
                     PELILFPRWFYINIYEMSSWSRAILVPLSIISARRPHCPVPAAASITELRVDGYRPAR
                     RSAWAALFHALDAGIKLVLRFPFRATREVAIKRCEQWIVERLRDSDGLGAIFPPIVNT
                     IFALHAVGYDLDHPTIRRQVDELERLELEEGDTLRVQPCFSPVWDTAIAINALIESGF
                     SPKDPAIRRAAEWLMHKRGNDEGDWRLKGGHAAGSGWFFEYANPFYPDCDTTSQVITS
                     LCKVDVFGTDTHARVQQAIFEAHQWHLSMQNPDGGWAAFDRGCDKRILLHVPYADHNA
                     MLDPSTADLTARGLESLAAIGFDRSYPPAARAIEFLKREQEADGSWFGRWGCNYLYGT
                     WLALTALERVGFDMRSSRVERAARWIGAVQNDDGGWGELPLSYDDPTSKGIGPSTASQ
                     TAWALLGLIAAGEVDSRRVVDGVEYLLREQRADGSWQETHWTGTGFPGVFYLRYHLYP
                     VYFPLLALGAFERAMDRRAKGRRSSRRLAVAPSARTVAV"
     gene            4082..5083
                     /gene="hpnH"
                     /locus_tag="VD788_06110"
     CDS             4082..5083
                     /gene="hpnH"
                     /locus_tag="VD788_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HXV75878.1"
                     /translation="MRFPLHIATDMIRYQVRQALAGNRRYPFVLMLEPLYTCNLACIG
                     CAVERHTGKLKDRLPVTTCLDAVDRCGAPSVSICGGEPTVYPELKQLIDGIIARRRHI
                     YLCTNALLLDERVFGVIPPHKRLTINVHLDGMRQTHDKVCDRPGVFDKAVAMIREAKR
                     LGYHVMTNTTVFKETDVNEVKALCELTTGLGVDGMLISPGYHYESVKHSIFLTRDEIR
                     KKFRKILGFSRHYRLSSTPMFLEFAAGYRDYRCSPWSTVTFTPRGWKGPCYLIGERHT
                     FSWDEFWNRTDWKYWESRTDDRCQNCAMHSGFEASAVQGAGRNPKDLGRLVAWNLFG"
     gene            5100..6098
                     /gene="hpnA"
                     /locus_tag="VD788_06115"
     CDS             5100..6098
                     /gene="hpnA"
                     /locus_tag="VD788_06115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HXV75879.1"
                     /translation="MVTLVTGASGFVGWHLAQALTRRGRAVRALVRPRSSIAGLAELP
                     VEIVRGDLRDVPSLERALDGVDELYHCAADYRLFVPDPSEMYATKVDGTDNLMRLAAD
                     RGVGRVIYTSTVGTLGLTADGTPGDERTPVTLAEMTGHYKRSKFLAEQRVREWADRGL
                     PVVIVNPSAPVGERDVKPTATGRMILDYLRGRMRAYVDTGLNLIDVRDCAEGHILAAE
                     RGHVGERYILGCRNMTLKEILETLARVSGGPAPRVRLPHWVPLTAAWIDTGVARLLRR
                     HPHIPLDAVRLAKHTMFFDSGKAVVELGLPQTPVEDALRRAVAWFRRSGYAGQAAA"
     gene            6095..6847
                     /locus_tag="VD788_06120"
     CDS             6095..6847
                     /locus_tag="VD788_06120"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75880.1"
                     /translation="MRAAIDPRPRRQETFGVVAAIPEEVAPLVARLGPGRRTRDGRTE
                     FIDGRLGARRVVVARTGEGRWNAECGARRLLERFPVSRLLCVGFCGGLSPDLVAGELV
                     IARRLVDPLRPLAAPLAPAIDGARPATLVTVDRMLCTPADKRSAWEAAGGGVATAADM
                     ETAAVARVAESRQVPYVALRVVSDAADEALPLDFNRFVDESGRLDRWAIVVRAVRRPS
                     LLPGLFELRGRSRRCAERLADAVCAMIESGGS"
     gene            6877..7896
                     /gene="fni"
                     /locus_tag="VD788_06125"
     CDS             6877..7896
                     /gene="fni"
                     /locus_tag="VD788_06125"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HXV75881.1"
                     /translation="MEERDRKAEHIRLALDARMQADGNHFDAYRFEHCALPEVDFDEI
                     DTRCRFLGRELSAPLLISCMTGGTDDAERINRNLARAAEETGVAVGVGSQRKALEDPS
                     RSGSFMIRPFAPTVPILGNLGAVQLNYGYGIRQCRAAVEMIDADALVLHLNPLQEAIQ
                     PEGQRNFATLLPKIGQIVSELEVPVIAKEVGCGISRVVAERLMRHGVEIVDTAGVGGT
                     SWARIEAARAGDAEIGERFADWGIPTPEALRGLRGLTGLTVIGSGGVRNGVDAAKAIA
                     CGADLVGLAYRFLRAADQSKEKVVESIRRIVEELKISMFCVGARTIAELGQVELRHSR
                     ADGVR"
     gene            7886..8845
                     /locus_tag="VD788_06130"
     CDS             7886..8845
                     /locus_tag="VD788_06130"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HXV75882.1"
                     /translation="MSAEMHNATTARPATGTPARLHELLVKTSRTFALNIPLLAEPTR
                     CEVTVAYLLFRVADTLEDSTAWTRRRKLKELARFAELLREPTEFTAARLATDWTSDPP
                     LGHEGYLELLAELPRVMRVHRELAPRSRQAIAKHTLRTVDGMSSFIARGAGDELRLRD
                     LADLKDYCYAVAGIVGEMLTELFIEQSEQLRPAAAELRELAPAFGEALQLVNILKDSC
                     GDRGEGRHFLPEGGDVAAVFRQARRDMGLAARYIAALDNAGAARGVLAFTALPLLLAR
                     ATLDCLERRGPGSKIRREDVARIVVDLKRALDRGELSALFARF"
     gene            8870..>10020
                     /locus_tag="VD788_06135"
     CDS             8870..>10020
                     /locus_tag="VD788_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HXV75883.1"
                     /translation="MTHFKLLQNLDGPADLKRLSIEQLAELAREIRTAICDQVSRSGG
                     HLAPNLGVVELTLALHYVFDFDRDRLLFDVGHQCYAHKLVTGRLRLLDKLRQRDGMAG
                     FPEPRESRYDLFSVGHAGTGVSTAIGMARGDLLNGEGDRRTVALVGDSSIVNGVSMEG
                     LNNAGTLKRQFLVVLNDNGMSIGLPQGALAGYFDKVRLSHRYTDLKDRAFELLKRVPA
                     GEKLEEIYNRLGEVTKAALHHTHIFERFGLLCVGPIDGHNIEGLIDMFNEVKDLDRPV
                     LVHVKTVKGKGFDFSSADPTTFHSPKPFQVNGCRVELKSSGRSFTAAYADAMAAMMER
                     DPRVVAVTAGMPDGTGVAQLISRFPERAFDVGIAESHAVDMCAGLAKTG"
BASE COUNT         1627 a         3329 c         3420 g         1644 t
ORIGIN      
        1 ccccccccac gacagggtgg cgaccagctc gtcccgttcc gcgcgcagct ccctggcgta
       61 ggcatcccac gcccggcgcc gcgcggcgtt ggccacctcc ttgtcttcct gttcgatatg
      121 gtccagctcg tcgcgatcga cgatgtgctg ggacagcatc cactcgcgca tgcgcgcgat
      181 gcagtcgtgc cgttcctccc acgccagccg gctctcgtcc ttgtagcgct cgtggctgcc
      241 ggaggtcgag tgtccctgtg gctgggtcat ctcggtgacg tggatgatcg acggaacgtg
      301 atcgcgtcgc gccacctcgg ccgcaccgag ataggtctcg cacagcgcgg catagtccca
      361 gccgggtacg cggtggacgt cgaaacccgg ggcgtcgcca ttctcgcgct ggaagccgga
      421 gagaaccgcc gagatgtcgt tgcggcagat ctgaaactcg ttgggaacag agatcccgta
      481 gtgatcgtcc cagatcgaga tgatcaccgg gacctgtagc acgccgatcg cgttgaccga
      541 ctcccagaac agaccctccg cgcagctcgc gttgccgatc gtgccgaagg cgatctcgtt
      601 gccgcgattg gacagccgcg gccatgcgga aagctcggcg atctggcgga acagcttcga
      661 ggcgtaacca agaccgacca gtcgcggcat ctgggacccg gtcggcgaca ggtcggccga
      721 ggagttcttc gactcggcga gcacgcgaaa cgcgccgtcg tcatccagca tgcgggtcgc
      781 gaaatgcgcg gtcatctgcc ggccggccga cgccggatcg gcgcggacgt cggcgtgggc
      841 gtacagttgg gcgaagaact cgtcgagcgt gagcaggccg gcggccaaca tgaaggtctg
      901 gtcccggtag tagcccgcgc gaaaatcgcc gtcgcggaag ctgcgcgcca tcgcgacctg
      961 ggcggcctcc ttgccgtcgc cgaagatgcc gaacttggcc ttgcccgaga agacctcctt
     1021 gcggccgagc agactcgcct ggcggctctg gaacatcacc cggtagtcac gaacgatgct
     1081 ctctgtcgtg atcttcaccg cggccctctc acgatggatt ccgtgcgcga cgcgccggtc
     1141 acccgaaccg ccagcgggcg ggcggacccg gcgggctgcg gtgatggtat cacgctgcgg
     1201 attccctccg attccgcgct tcaggccccg gtcttgagac cgtcgagcaa acgttggatc
     1261 gtgcgtcgga aaaccccgcg gaactgctcg ggatcctgcc cgaaccgctc cagccgaaac
     1321 aacgtcacca agccgtgcgc ctcggcccag accatgatcg ccgtttcgag cggatcgctc
     1381 ttgacgaaca ccccttgctc catgcacagg tgcacttgct gcacggcgaa catgaacgtg
     1441 ctgcggttgt ggcgcgcgag ttcctcggtc aactcgatcc cgtggctcgg cacgagaaag
     1501 gcaaaatcga agtaacgagg ctgctcgagc gcgaagtcga gatagtactc gatcatccgc
     1561 tgtaggcggt cgaacggccg ctcggcacgg aacgcgggtt cgaggtagcg ctgcaaagtc
     1621 atcaaaccga cgttgatgat ctcgctcagc agctcgtcct tgtccttgaa gtgacggtag
     1681 atcgccggcg cggtgacgcc gacccggtcg gcgactttcc gcatcgacac gccgtcgacg
     1741 ccctcggcca gaaacagctc ctgcgccgcg gccgagatcc gttcgcgcag cgtgttcggt
     1801 ccgcgaacgc tgttcacgga tcggcgctcc catcgcccgc ggtgcgcttt gtccgagtgg
     1861 tcgttcatcg aaactacgtc acgctcccca gccactgtag tcgtgaacgc cgcggcttgg
     1921 caagcgaccg gcgcgcgtcc gctcgtcgcg aggcagcgag gtggtgacgg cgaacagcgt
     1981 tcactccgcg gtgctccggg acgaagagtc cgggaaaacg gccgccgtgc tctaccgggg
     2041 cactggcgat tgtcataccc taggacggat tcacgaggag gacccgcgcg ttgagccgcc
     2101 agctgaccac cgccgtaccg gtcaccgaga ccccggacga tctgcgtttc cgtgccagag
     2161 aagcgatcga gagttcccag cgccacctgc tcgcgatcca gaacgaagac ggtcactggt
     2221 gcggagagct cgaaggcgac acgattctcg agtcggagta catcctcgcc atgcacttcc
     2281 tcggccgcac gcacgaggac cgggtgcgca aggccgccaa ctatctccgt gacaaacagc
     2341 tgccgggcgg cggctgggga gtctaccccg ggggcccggc cgaggtcagt gcctcgacca
     2401 aggcctactt cgtcctcaag ctcgtcgggg acgatccgga cgctccgcat atgtcggcgg
     2461 cgcgacggac gattcgtgcg ctgggtggtc tcgacgcctg caactcgttc accaagatct
     2521 acctcgcggt cttcggccag ctcgactggg acgactgccc gtccgttcca ccggagctga
     2581 ttctctttcc acgctggttc tacatcaaca tctacgagat gtcgtcgtgg tcgcgcgcga
     2641 ttctggtacc gctgagcatc atcagcgcgc gacgcccgca ttgtccggtg ccggcggcgg
     2701 cctcgatcac ggagctgcgc gtcgatggct atcggccggc acgccgttcg gcgtgggccg
     2761 cgctgttcca cgctctcgat gccgggatca agctcgtcct caggttcccg tttcgcgcga
     2821 cacgcgaggt ggcgatcaag cgatgcgagc agtggatcgt cgagcgactg cgcgacagcg
     2881 acgggctcgg cgcaatcttc ccgccgatcg tcaacacgat tttcgccttg cacgcggtcg
     2941 gctacgacct cgatcacccg acgatccgac ggcaggtcga cgagctcgag cgactcgagc
     3001 tcgaggaagg cgacacgctg cgggtgcagc cgtgcttctc gccggtgtgg gacaccgcaa
     3061 tcgcgatcaa cgccctgatc gaatcgggat tctcgccgaa ggacccggcg atccggcggg
     3121 cggccgagtg gttgatgcac aagcgcggca acgacgaggg cgattggcgg ctgaagggcg
     3181 gccacgcggc ggggtccggc tggttcttcg agtacgccaa cccgttctac cccgattgtg
     3241 acaccacgtc gcaggtgatc acgtcgttgt gcaaggtcga cgtcttcggc accgacacgc
     3301 acgcgcgcgt gcagcaggcg atcttcgaag ctcatcagtg gcacctgtcg atgcagaacc
     3361 cggacggcgg ctgggcggcg ttcgaccgcg gctgcgacaa gcggattctg ctccacgtgc
     3421 cgtatgccga tcacaacgcg atgctcgatc cgagcaccgc cgacctgacg gctcgcgggc
     3481 tcgaatcgtt ggctgcgatc ggcttcgatc ggagctaccc ccccgccgca cgcgcgatcg
     3541 agttcctcaa acgggagcag gaggcggacg gctcgtggtt cgggcgctgg ggttgcaact
     3601 acctgtacgg cacctggctg gcgttgacgg cgctcgagcg cgtgggattc gacatgcgat
     3661 cgagccgcgt cgagcgcgcc gcgcgctgga tcggcgccgt gcagaacgac gacggcggtt
     3721 ggggtgagct gccgttgtcc tacgacgatc cgacgagcaa gggtatcggg ccgagcacgg
     3781 cctcgcagac cgcctgggcc ttgctcggtc tgatcgcggc cggcgaggtc gattctcgtc
     3841 gggtggtgga cggcgtcgag tacctgctgc gcgagcagcg cgcggacgga tcgtggcagg
     3901 agacgcattg gaccgggacg gggttccccg gcgtcttcta cttgcgctac cacctctacc
     3961 cggtctattt cccgttgctc gccctgggcg cgttcgaacg cgcgatggac cgacgagcga
     4021 agggacgacg ttcgagccgg cggttggccg tcgcgccgag cgctcggacg gtggccgtgt
     4081 gatgagattc ccgctgcaca tcgccacgga catgatccgc taccaggtca ggcaagccct
     4141 ggcgggcaat cggcgctatc cgttcgtgct gatgctcgag ccgctgtaca cctgcaacct
     4201 ggcctgcatc ggctgcgcag tcgagcgcca caccggcaag ctgaaggacc ggctaccggt
     4261 cacgacctgc ctcgacgcgg tcgaccgctg cggcgctccc tccgtctcga tttgcggcgg
     4321 cgagccgacc gtctaccccg agctgaagca gttgatcgac ggcatcatcg cccgccgccg
     4381 gcacatttat ctctgtacca acgccttgct gctcgacgag cgggtgttcg gcgtgatccc
     4441 gccgcacaag cgcctgacga tcaacgtgca cctcgacgga atgcggcaga cgcacgacaa
     4501 ggtctgcgat cgacccggcg tcttcgacaa ggcggtcgcg atgatccgcg aggcgaaacg
     4561 tctgggctat cacgtgatga cgaacacgac cgtgttcaag gaaaccgacg tcaacgaggt
     4621 caaggcgttg tgcgagctga cgacgggact cggtgtcgat ggcatgctga tctcgcccgg
     4681 gtaccactac gagtcggtca agcacagcat cttcctcacc cgtgacgaga tccggaagaa
     4741 gttccgcaag atcctcggct tctcgcgaca ctaccgtctg agctcgaccc cgatgttcct
     4801 cgagttcgcc gcgggctacc gcgattaccg ctgctcgccg tggagcaccg tcaccttcac
     4861 accccgcgga tggaagggac cgtgctacct gatcggcgag cgccatacgt tttcatggga
     4921 cgagttctgg aaccggaccg attggaagta ctgggagtcg agaacggacg accgctgcca
     4981 gaactgcgcc atgcattcgg ggttcgaggc gtcggcggtg cagggcgccg gccggaaccc
     5041 gaaggacctc ggtcggctgg tcgcgtggaa cctgttcggc taggagcaaa cggacgcgga
     5101 tggtgacact ggtcacgggc gcatcgggct tcgtcggttg gcatctggcg caggccctga
     5161 cgcggcgcgg ccgcgcggtg cgggccctcg tccgcccgcg cagctcgatc gcgggtctgg
     5221 ccgagctgcc ggtcgagatc gtccgcggcg acctgcgcga cgtgccgtcg ctcgaacgcg
     5281 cactcgacgg cgtcgacgag ctgtaccact gtgccgccga ttaccggttg ttcgttcccg
     5341 acccgtcgga gatgtacgcc accaaagtcg acggcaccga caacctgatg cggctcgcgg
     5401 ccgaccgcgg cgtcggccgt gtgatctaca ccagcacggt cggtacgctc ggcctgaccg
     5461 cggacggcac accgggcgac gagcggacac cggtgacgct ggccgagatg accggccact
     5521 acaaacgaag caagttcctc gccgaacagc gcgtgcgcga atgggccgat cgcgggctgc
     5581 ccgtcgtgat cgtcaacccg tcggcgcccg tcggcgagcg cgacgtgaaa ccgaccgcca
     5641 ccggtcggat gatcctcgac taccttcgcg gcaggatgcg ggcctacgtc gacaccgggc
     5701 tgaacctgat cgacgtgcgg gattgcgccg aagggcacat tctcgccgcg gaacgtggtc
     5761 acgtcggcga gcgttacatc ctcgggtgcc gcaacatgac gttgaaggag atcctggaga
     5821 cgctggcgcg ggtctcgggt ggcccggcgc cgcgcgtgcg gctgccgcat tgggtgccgt
     5881 tgacggcggc ctggatcgac accggcgtgg cacggctgct gcgacgccac ccgcacattc
     5941 cgctcgacgc ggtgcggctg gcgaagcaca cgatgttctt cgattcgggc aaggcggtcg
     6001 tggagctggg tctgccgcag accccggtcg aggacgcgtt gcgacgggcg gtcgcctggt
     6061 ttcgccgatc gggttacgcc ggacaggcgg ccgcatgagg gccgcgatcg acccgcgccc
     6121 gcgccggcag gagaccttcg gcgtcgtcgc cgcgatcccg gaggaggtcg cgccgctggt
     6181 cgcgcggcta ggcccggggc gacgcacgcg cgacggccga acggagttca tcgacggccg
     6241 cttgggcgct cgccgcgtcg tcgtcgcgcg cacgggcgag ggccgctgga acgccgagtg
     6301 cggcgcgcgc cgtctgctcg aacgattccc ggtctcccgt cttctgtgcg tcggtttttg
     6361 cggcggcctg tcaccggatc tcgtcgccgg ggagctcgtg atcgcccgcc ggctggtcga
     6421 cccgctgcgg ccgcttgcgg cgccgctcgc gcccgcgatc gacggcgcgc gtccggcgac
     6481 cttggtcacg gtcgaccgca tgctctgcac cccggcggac aagcgcagcg cctgggaggc
     6541 ggcgggaggc ggggtggcga cggctgccga catggaaacc gccgcggtgg cccgggtggc
     6601 cgaatcgcgc caggtgccat acgttgcact gcgggtggtg agcgacgccg cggacgaggc
     6661 gctcccgctc gatttcaacc ggttcgtcga cgagagcgga cggctcgacc ggtgggcgat
     6721 cgtcgtacgc gcggtgcgac gaccgagcct gctccccgga ctgttcgagc tacgcggccg
     6781 ctcgagacga tgtgccgaga ggctggcgga cgccgtgtgc gcgatgatcg aatccggtgg
     6841 gtcatgagcc gctcggttcc ggcggacgac cgtcgcttgg aggaacgcga ccgcaaggcg
     6901 gagcacatcc gcctggcgct cgacgcgcgc atgcaggccg acggcaacca cttcgacgcc
     6961 taccgcttcg agcactgcgc gctccccgag gtcgacttcg acgagatcga cacccgctgc
     7021 cgttttctcg ggcgtgaact gtcggcgcca ttgctgatat cctgcatgac cggcgggacg
     7081 gacgacgccg agcggatcaa tcggaacctg gcccgcgcgg ccgaggagac cggtgtcgcc
     7141 gtcggcgtcg gctcgcaacg caaggcgctg gaggacccgt cgagatcggg cagcttcatg
     7201 atcaggccgt tcgcgccgac ggtgccgatt ctgggcaacc tgggagccgt gcagctcaac
     7261 tacggatacg ggattcgcca atgccgggcg gcggtcgaga tgatcgacgc cgatgcgctg
     7321 gtattgcatc tcaacccgtt gcaggaggcg atccagcccg agggacagcg caacttcgcg
     7381 accttgctgc cgaaaatcgg ccagatcgtc tcggagctcg aggtgccggt catcgccaaa
     7441 gaagtcggct gtggaatctc gcgcgtggtt gccgagcggc tgatgcggca cggtgtcgag
     7501 atcgtcgaca ccgcgggcgt cggcggtacg agctgggctc ggatcgaggc ggcccgagcg
     7561 ggcgacgccg agatcggcga acggttcgcc gattggggca ttccgacgcc ggaggcgctc
     7621 cgcggcctcc gcggcctgac cggtctgaca gtgatcggca gcggcggggt caggaacggg
     7681 gtcgacgcgg ccaaggcgat cgcctgcggc gccgacctcg tcggtctggc ctatcgcttc
     7741 ctccgcgccg cggaccaatc gaaggaaaaa gtggtcgagt cgatccgccg catcgtcgag
     7801 gagctgaaga tcagcatgtt ctgcgtggga gcgcggacga tcgccgagct cgggcaggtc
     7861 gagctgcgcc actcgagggc cgacggtgtc cgctgagatg cacaacgcga ccaccgcgcg
     7921 acccgcgacg ggcacaccgg ctcggctgca cgagctgctg gtcaagacga gccgcacatt
     7981 cgcgctgaac atcccgctgc tcgccgagcc gacgcgctgc gaggtgacgg tggcctacct
     8041 gctgttccgg gtcgccgaca cgctcgaaga ctcgacggcg tggacccgcc ggcggaagtt
     8101 gaaggagctc gcccgattcg ccgagctgct gcgcgagccg accgagttca ccgccgcgcg
     8161 gctggccacc gactggacgt ccgatccacc cctcgggcac gagggctatc tcgagctgct
     8221 ggccgagctg cctcgcgtga tgcgtgtcca ccgcgagctc gcgccgcgat cgcgacaagc
     8281 gatcgccaaa cacacgttgc gcacggtcga cggtatgagc tcgttcatcg cgcgcggggc
     8341 tggcgacgag ttgcgcctgc gcgacctcgc cgatctcaaa gactactgct acgcggtcgc
     8401 cggcatcgtc ggcgagatgc tgaccgagct gttcatcgag cagagcgaac aactgcggcc
     8461 cgcggccgcc gagctccgcg agttggcacc cgcgttcggc gaagcgctgc agctggtcaa
     8521 catcttgaaa gattcgtgcg gcgaccgcgg tgagggacgt cacttcctgc ccgagggcgg
     8581 cgacgtggcc gcggtttttc ggcaggcgcg gcgcgacatg ggcctcgccg cgcgctacat
     8641 cgcggcgctc gacaacgcgg gtgccgcgcg cggggtactg gccttcaccg ccttgccgtt
     8701 gttgttggcg cgagcgacgc tcgactgcct cgagcgccga ggtccggggt ccaagatcag
     8761 acgcgaggac gtggcccgga tcgtcgtcga tctcaagcga gcgctcgacc gcggcgagct
     8821 gtcggcgctt ttcgctcggt tttagcccgt ctctgatcaa gaggtgctca tgacacattt
     8881 caagttgctg cagaatctcg atggtccggc cgacctcaaa cggctgtcga tcgaacagct
     8941 ggccgagctg gctcgcgaga ttcggaccgc gatctgcgat caggtctccc gctcgggcgg
     9001 acatctcgct cccaacctgg gcgtggtgga actgacgctc gcgctgcatt acgtcttcga
     9061 cttcgaccgc gatcgcctgc tgttcgacgt cgggcaccaa tgctacgccc acaagctcgt
     9121 caccgggcgt ctgcggttgc tcgacaagct gcggcagcgg gacgggatgg ccggttttcc
     9181 cgagccgcgt gagagccgct acgacctgtt cagcgtcggc catgccggaa ccggcgtgtc
     9241 gacggcgatc gggatggccc gcggcgacct gctcaacggc gagggtgacc gccgcacggt
     9301 cgcgttggtc ggtgattcgt cgatcgtcaa cggcgtgtcg atggagggtc tgaacaacgc
     9361 cgggacgctc aaacgccagt ttctcgtcgt gctcaacgac aacgggatgt cgatcggact
     9421 gccgcagggg gcgctcgcgg gctacttcga caaggtccgc ttgagccacc gctacaccga
     9481 cctgaaggac cgtgcgttcg agttgctcaa acgggttccg gccggcgaga agctcgagga
     9541 gatctacaac cgtctcggcg aggtgaccaa ggccgcgttg caccacaccc acatcttcga
     9601 acgcttcggg ctgctgtgcg tcgggccgat cgacgggcac aacatcgagg ggttgatcga
     9661 catgttcaac gaggtcaagg acctcgatcg acccgtgctg gtccacgtca agacggtcaa
     9721 gggcaagggc ttcgatttca gcagcgccga cccgacgacc ttccactctc ccaagccgtt
     9781 ccaggtcaac ggctgtcggg tcgagctgaa atcgagcggg cggtcgttca ccgcggccta
     9841 cgccgacgcg atggcggcga tgatggaacg cgacccacgg gtggtcgcgg taacggcggg
     9901 catgccggac gggacggggg tcgcacagtt gatcagccgc ttccccgagc gagcgttcga
     9961 cgtcggcatc gccgagtccc acgccgtcga catgtgcgcc ggcctggcca agaccgggtt
//
LOCUS       DASPGN010000215         7214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_153831, whole genome shotgun sequence.
ACCESSION   DASPGN010000215 DASPGN010000000
VERSION     DASPGN010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153831"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1166)
                     /locus_tag="VD788_06140"
     CDS             complement(108..1166)
                     /locus_tag="VD788_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75884.1"
                     /translation="MSTDPLARRRTLGSLFACLLLPWCVPASAVADHASLTLHVEDAM
                     GYPGGRIAFVIRAYASRPIGQGQLCFAARPRAEVPRDGEGDTTTETPLLAPTESIVFS
                     QAGDAEVELAPTEDEPRRFVLRFVSPSASVNVYAGPLAVVFFELRDELVPGAEYDLVL
                     DSNDTQMIDDRGDNLRIEAIDGTLSIRQPAEPHRLHARAAGAAPGEIASIGLYSFEPV
                     RWAAGRVALRYDPQVVAGPPIVLIDPRHGRAEFTYDVPTAGLLLVEFTSHDASLNQVP
                     GAVVEVYAALSRRARPLTHSAVSLDPALTWIVGADGRLVPLELAGTELPVLIPASETG
                     RRHAAPDSALAFGYVAGD"
     gene            complement(1202..1942)
                     /locus_tag="VD788_06145"
     CDS             complement(1202..1942)
                     /locus_tag="VD788_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75885.1"
                     /translation="MSRRSDSLDDDLARALAGSSDCPPPETFERGRWDSLTFAERGRI
                     GRHADRCAACSAERELARAFDHPKATGLDADEAFVAERLDRLRPSSTLARAGGRSRFP
                     ADWRWVSAAALVGVALVAGLVWRGTPSPAPLLPDPPPPSTERGIHLRFDAPRGDLARA
                     PGEARWEAHPGAESYLLRLVAIDGKVIWSTTAPASSVELPAVLVDRLAAHVTYRFEVD
                     ALDAQGRVVAASAAVGFRIRPETGGGGS"
     gene            complement(1939..2637)
                     /locus_tag="VD788_06150"
     CDS             complement(1939..2637)
                     /locus_tag="VD788_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV75886.1"
                     /translation="MDVYFRSVSAAVLPAVVTGGRPRMNERDRYRELSRASDDQLVSN
                     ARRALGPARAVCLETLYRRFYPRVARWCVKVSRDREEAADLAQEVFLRVHSRFDTFRG
                     DCAFSTWLYTVMRSVAINRGLTLKRIADRTGPASAIDEAADGGRDPAQHVERDERMRR
                     LRRLIDSRLEPLEARVLYLHYVDGMSLPAITRLLGLANKSGAKAFVVSGKRKPRRGLT
                     EEDRGPRALLRVSS"
     gene            complement(2768..3991)
                     /locus_tag="VD788_06155"
     CDS             complement(2768..3991)
                     /locus_tag="VD788_06155"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXV75887.1"
                     /translation="MNVLMISPGYPGQMPYFTRGLAAVGARTFGVGDQPDSALAHDVR
                     RALVAYLQVPDLWDEQAVVRRVLDEARSHELRFDRVECLWEPGMILAARLRQALDARG
                     MRVEQTVPFRDKEVMKQLLDRAGIRTPWHARCTTGNEVRAAVARVGYPAIVKPIAGAG
                     ATDTHRVDDDEQLALVIASVGHVPEVSVEEFIAGDEYTHDTICAGGRMLYENVARYTT
                     KPIEERKHQWVSPGSVCLREIDAAELAGGRQMGRRVLAALGFRTGFTHMEWFRKPDGE
                     IVFGEIAARPAGAHLIEVMNFASDVDLFRGWAEAVVHGRLSQPIERRYNAALIGKRAR
                     GSGRIVGSEGLDRLRHELGSALCKVQMNPVGTPAGDWKRGAPADGFAIVRHPDLPTTL
                     ELAARVAREWQIQAG"
     gene            4083..5189
                     /locus_tag="VD788_06160"
     CDS             4083..5189
                     /locus_tag="VD788_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HXV75888.1"
                     /translation="MEQPREFELAEPDRGSIPVRVTPASATGARRWLAAIEDRRRWLA
                     DCLERYGALLFRGFDVSSAEALAAFTRAAGFRLADYRRGITPRRAVHGGVYTATDAPP
                     AFPIPLHNEMSYTSVYPTGVALCCETPPTRGGRTPIADMARVLDRLPVALVARFDALG
                     VRYRQHVPSSSSSRRARSWQQMFDTEDRSEVERVCAAQGIDHRWVGDEIALDSVRPAL
                     LTHPRSGSRVWFNQVQVFHPSFSHELFHHRRWLLGLAVRAHEWTRAPRISCCHGDGTA
                     IARRTMYVVRRALWAETRRFDWRRGDLLLLDNLRVAHGREPYRGARRVLAALISGAGG
                     AGGGLGGQSDDAAPAAAGRAPAAGSSGGAPHRSS"
     gene            complement(5105..>7214)
                     /locus_tag="VD788_06165"
     CDS             complement(5105..>7214)
                     /locus_tag="VD788_06165"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HXV75889.1"
                     /translation="CRYPLELDGLAPLGRWIVARRISIYCSVATLFRQFVRTLGGERF
                     TDLRLIKLGGEVVFRSDFEAFRRCFEPGTLLSCGLAATEVGAIRQFFLDHRSTVDDLR
                     VPCGHPVDGVEVLIVDERACELPRGSVGEIAVRSRYLSPGYWRRPERNERVFVPDPAD
                     PTRRVYLTGDLGYVRDDGQLIHAGRKDHQVKIRGNRVEIPEVEAALAELRGVRDAAVT
                     LLADPERGNRLHAFVATGGERATEAELRQSLAGCLPAFMIPSAFTIVERLPLLPNGKI
                     DRARLAEIEAPPEPAVASAGLAQSATARELACLWDELLDRPAGPDDDFFEAGGDSLAL
                     LQLMVAIEHRFGVRFPLNVILDCPSIRSLAARIDARRSGGDRAETLAPITDDESDEIA
                     LSVMPIQPAGELPPLFFVAPAGGTVLPYYRLAHLLGRSQPFFGLQVAPPARRGQPFDI
                     ADVARRFLRMIDRLVPSGPCRFGGWSFGGFVAWEMAAQARRSALDVETVLLVDTDATI
                     AGHEPQGLDRFKGLLTVLQMFRHSRPFLREIIYVRLAANMRRQGADAPAGRLARLAWH
                     AGIKHADVSEVIGADPRLLDLQFVPVRGMANLRSYMRSIHAFTPPVVAQRVDLFMPTV
                     DVGRRERLHARAVADRWSELSSGRLHHHPLTGNHFTIFHPPHIERMAAIMGQLLRCGA
                     PPLEPAAGARPAAAGAASSD"
BASE COUNT         1066 a         2569 c         2619 g          960 t
ORIGIN      
        1 ggcctacgtg ctggtcggcc gcggtcaccc cgtcgccccg gccgcgcgca cggcttggtt
       61 gctcccggcc gcgatcgtcg ccgtcgtggc ggccgccgtc gtcgcgatca gtcgcctgcg
      121 acgtagccga aggcgagcgc cgagtcggga gcggcgtgcc gacgacccgt ctcggacgcc
      181 ggaatgagca ccggcagctc ggtgccggcc agctcgagcg gcaccagtcg gccgtctgct
      241 ccgacgatcc acgtcagcgc ggggtcgagc gagacggccg agtgggtgag cggacgcgcc
      301 cgccgcgaca gcgcggcgta gacctcgacg accgcgcccg gtacctggtt cagcgatgcg
      361 tcgtgcgagg tgaattcgac gagcagcagg cccgccgtcg gcacgtcgta ggtgaactcc
      421 gcgcggccgt ggcgcggatc gatcagcacg atcggcgggc cggcgacgac ctgcgggtcg
      481 tagcgcaagg cgacgcggcc cgcggcccac cggaccggct cgaacgagta gaggccgatc
      541 gaggcgatct cacccggggc agctcccgcc gcccgcgcgt gcaagcggtg gggctcggcc
      601 ggttgtcgga tcgacagcgt tccgtcgatc gcctcgatcc gcaggttgtc gccacggtcg
      661 tcgatcatct gcgtgtcgtt gctgtcgagc accaggtcgt actccgctcc agggacgagc
      721 tcgtcgcgca gctcgaagaa gacgacggcg agcggtccgg cgtagacgtt gacgctggcc
      781 gacggcgaga cgaagcgcag gacgaagcgt cgcggctcgt cctcggtcgg cgcgagctcg
      841 acctcggcgt cacccgcctg gctgaagacg atcgattcgg tgggcgcgag cagcggggtc
      901 tcggtcgtcg tgtcgccctc gccgtcgcgc ggcacctcgg cgcgcggtct cgccgcgaaa
      961 cacaactgac cctgaccgat cgggcgggag gcataggccc ggatcacgaa ggcgatgcgt
     1021 ccgccgggat agcccatcgc gtcttcgacg tgcagggtga gcgaggcatg gtcggcgacc
     1081 gcggaggccg gaacgcacca cggcaggagc agacaagcga agagcgaccc gagcgtgcgg
     1141 cgccgggcga gcggatcggt ggacatcgtc gcgttcctcc tctcgtcacg accgcccgtc
     1201 gctagcttcc gccaccgccc gtctcgggac ggatgcggaa cccgaccgcc gccgatgcgg
     1261 cgacgacccg gccctgcgca tcgagcgcgt cgacctcgaa gcggtaggtc acgtgggcgg
     1321 ccagccgatc gacgagcacc gccggaagct cgaccgagct cgccggcgcc gtcgtgctcc
     1381 agattacctt accgtcgatc gcgaccaggc gcagcaagta cgactcggcg cccggatgcg
     1441 cctcccaacg tgcctcgccc ggagcgcggg ccaggtcgcc acgcggcgcg tcgaacctca
     1501 gatggatgcc gcgctcggtg gacgggggcg gcgggtcggg cagcagggga gccggggacg
     1561 gcgttccccg ccagaccagc cccgcgacga gggcgacccc gaccagcgcc gccgccgaga
     1621 cccagcgcca gtcggcgggg aagcgactgc gtccgcccgc acgcgcgagg gtcgaggacg
     1681 gtcgcagtcg atcgagccgt tcggcgacga atgcctcgtc ggcgtcgaga ccggtcgcct
     1741 tcgggtggtc gaacgcgcgg gccagctcgc gctcggccga gcaggccgcg catcgatcgg
     1801 cgtggcgccc gatccggccg cgctcggcga acgtgaggga gtcccagcga ccgcgctcga
     1861 acgtttcggg cggtgggcag tcgctgctgc ccgccagcgc gcgcgccagg tcgtcgtcga
     1921 gcgaatccga acgccggctc atgacgacac ccggagcaag gcgcgcgggc cccggtcctc
     1981 ctcggtcagc ccgcgtcgcg gctttctctt gccgctgacg acgaacgcct tggcgccgga
     2041 cttgttcgcc aacccgagca aacgcgtgat ggccggcagg ctcatcccgt cgacgtagtg
     2101 caggtagagc acgcgtgcct cgagcggctc gagccgggag tcgatcagtc gtcgcagtcg
     2161 tcgcatgcgc tcgtcgcgct cgacgtgctg ggccggatcg cgcccgccgt cggccgcctc
     2221 gtcgatcgcc gaggccgggc cggtgcggtc ggcgatccgc ttcaaggtca gcccgcgatt
     2281 gatcgccacg ctgcgcatga ccgtgtagag ccaggtcgag aaggcacaat cgccgcgaaa
     2341 ggtgtcgaac cgcgaatgca cgcgcaggaa gacctcctgc gccaagtcgg cggcctcctc
     2401 ccgatcgcga ctgaccttga cgcaccagcg cgccacccga gggtagaacc ggcgatacag
     2461 cgtctcgaga cagaccgcac gggccgggcc gagcgcgcgg cgcgcgttgg acaccagctg
     2521 gtcgtcgctg gcccggctga gttcccggta ccggtcgcgc tcgttcatcc tcggtcttcc
     2581 gcccgtgacc accgccggca gcaccgccgc cgacaccgag cggaagtata cgtccactgg
     2641 gaagatccgg tcacgtgacg ggatcgtgat cgcgccggcc gggcacgcgg atggcgtcat
     2701 cgaggttacc ccggcggatt ggacagggcg agcgggccgc cggaacaaca ccgggtgcga
     2761 tccggggtca gccggcctgg atctgccact cgcgcgccac gcgcgccgcc agctcgagtg
     2821 tggtcggaag gtcggggtgg cgcacgatgg cgaagccgtc ggccggggcg ccgcgcttcc
     2881 agtcgccggc cggtgtcccg accgggttca tctgtacctt gcacagcgcc gagcccaatt
     2941 cgtggcggag ccggtcgagc ccctcgctgc cgacgatgcg ccccgagccg cgcgcacgct
     3001 tgccgatcaa cgcggcgttg taccgtcgct cgatcggctg cgacagacgc ccgtgtacga
     3061 cggcctcggc ccagccgcgg aacaggtcga cgtccgaggc gaaattcatc acctcgatca
     3121 ggtgcgcgcc ggcggggcgt gccgcgatct cgccgaagac gatctccccg tcgggtttgc
     3181 ggaaccactc catgtgggtg aagcccgtgc ggaagccgag cgcggccagc acgcggcggc
     3241 ccatctgcct gccgccggcg agctccgccg cgtcgatctc acgcaggcag accgagcccg
     3301 ggctgaccca ctggtgcttg cgctcctcga tcggcttggt cgtgtaccgc gcgacgttct
     3361 cgtagagcat ccgcccaccg gcgcagatcg tgtcgtgggt gtactcgtcg ccggcgatga
     3421 actcctcgac gctgacctcg ggtacgtgcc cgaccgaggc gatcaccagc gcgagctgct
     3481 cgtcatcgtc gacgcgatgg gtgtcggtcg cccccgcgcc ggcgatcggt ttgacgatcg
     3541 ccgggtaacc gacccgcgcg accgccgccc ggacctcgtt gccggtggtg cagcgggcgt
     3601 gccacggcgt gcggatgccg gcccggtcga gcagctgctt catcacctcc ttgtcacgaa
     3661 acggcacggt ctgttcgacg cgcatgccgc gcgcatcgag cgcctggcgc agccgcgcgg
     3721 ccaggatcat ccccggctcc cacaggcact cgacccggtc gaagcgcagc tcatgactcc
     3781 gcgcttcgtc cagcactcgt cgcacgaccg cctgctcgtc ccacaggtcg ggcacctgca
     3841 gataagccac gagcgcccgg cggacgtcgt gcgccagcgc ggagtcgggc tggtcgccga
     3901 cgccgaacgt ccgcgcgccg accgcggcga ggccccgggt gaagtagggc atctgccccg
     3961 ggtagcccgg cgagatcatc agcacgttca tcgacgcacc atgctaaacc atccgctgcc
     4021 cggcgcgtgc taacctcgat cgcccgcagc gcgggccccg cgggccggtg agtgacgagc
     4081 cgatggagca gccgcgagag ttcgagctgg ccgagcccga cagggggtcg atccccgtac
     4141 gggtcacgcc cgcgtccgcg accggcgcgc ggcgctggct cgctgcgatc gaggatcgtc
     4201 gccgctggct cgccgactgc ctcgagcgct acggcgcgtt gctgttccgc ggtttcgacg
     4261 tatcgagcgc cgaagcgctc gcggcgttca cccgtgccgc cgggttccgg ctggccgatt
     4321 accgtcgcgg catcacgccg cgtcgtgcgg tgcacggcgg ggtctacacg gcgaccgacg
     4381 caccgccggc gtttcccatt ccgttgcaca acgagatgtc ctacaccagc gtctacccga
     4441 ccggcgtcgc actctgctgc gagacgccgc cgacgcgcgg cgggaggaca ccgatcgccg
     4501 acatggcccg cgtgctcgat cgtctgccgg tggcgctggt cgcgcggttc gacgcgctcg
     4561 gcgtccgcta ccgccagcac gtgccgtcgt cgtcgtccag ccgccgcgca aggagctggc
     4621 agcagatgtt cgacaccgag gaccggagcg aggtcgagcg cgtgtgcgcg gcccagggga
     4681 tcgaccaccg ctgggtcggt gacgagatcg cgctcgacag cgtccgaccg gcgctgctga
     4741 cgcacccgcg atcggggagc cgggtctggt tcaaccaggt ccaggtgttc cacccgagct
     4801 tttctcacga gctgttccat caccgccgct ggctgctggg actcgcggtc agggctcacg
     4861 agtggacgcg cgcgccgcgg atcagctgct gccacggcga cgggacggcg atcgcacgtc
     4921 ggacgatgta cgtggtgcgg cgggcgctgt gggccgagac gcgccgattc gactggcggc
     4981 gcggcgatct gctgctgctc gacaacctgc gcgtcgcgca cgggcgggaa ccgtaccgcg
     5041 gcgctcgccg cgtgctggcc gcgctgatct ccggggcggg gggtgccggc ggcggccttg
     5101 gcggtcagtc ggacgacgcg gcgccggccg ccgccggccg agctccggca gcgggctcga
     5161 gcggcggggc gccgcaccgc agcagctgac ccatgatcgc cgccatccgc tcgatgtgag
     5221 gcggatggaa gatggtgaag tggttgccgg tgagcggatg atgatgcaac cggccgctgg
     5281 acagctcgga ccagcggtcg gcgacggcgc gcgcgtgcag gcgctcgcga cgacccacgt
     5341 cgaccgtcgg catgaacaga tcgacgcgct gcgcgacgac cggtggcgtg aaggcgtgga
     5401 tcgagcgcat gtaggatcgc agattggcca tgcctcgtac ggggacgaac tgcaggtcga
     5461 gcagccgcgg gtcggcgccg atgacctcgg agacgtcggc gtgtttgatc cccgcgtgcc
     5521 aggccagccg cgcgagccga ccggccggcg cgtcggcgcc ctgccgacgc atgttggccg
     5581 ccagccgcac gtagatgatc tcccgcagga acggccggct gtggcggaac atctgcaata
     5641 cggtcaacag ccccttgaag cggtcgagac cctgcggctc gtgacccgcg atcgtggcgt
     5701 ccgtgtcgac gagcaggacg gtctcgacgt cgagcgcgct gcgacgggcc tgggccgcca
     5761 tctcccaggc gacgaagccg ccgaacgacc agccaccgaa gcggcacggt cccgacggca
     5821 caagacggtc gatcatgcgg aggaagcgcc gggcgacgtc ggcgatgtcg aacggctgcc
     5881 cgcgccgggc gggtggggcc acctgcaggc cgaagaacgg ctgcgatcgc ccgagcaggt
     5941 gggccagacg gtagtagggc agcaccgtcc cgccggccgg ggcgacgaag aacaacggcg
     6001 gcagctcgcc ggcgggctgg atcggcatca ccgacagcgc gatctcgtcc gactcgtcgt
     6061 cggtgatcgg agcgagcgtc tccgcgcggt cgccgcccga gcgcctggcg tcgatccgcg
     6121 cggcgagcga tcggatcgac gggcagtcga ggatcacgtt cagcgggaag cggacaccga
     6181 accggtgttc gatcgcgacc atcagctgca gcagggccag cgagtcgccg ccggcctcga
     6241 agaaatcgtc gtcgggaccc gcgggtcgat cgagcagctc gtcccacaag caggcgagct
     6301 cgcgcgccgt cgccgactgg gcgagaccgg ccgacgcgac cgccggttcg ggcggcgctt
     6361 cgatctcggc cagccgcgcg cggtcgatct tgccgttcgg cagcagcggc agccgctcga
     6421 cgatggtgaa cgccgacggg atcatgaacg ccggcagaca cccggcgagc gattggcgca
     6481 gctcggcctc ggtggcgcgc tcgcccccgg tcgcgacgaa cgcgtggagg cggttcccgc
     6541 gctcggggtc ggcgagcagc gtaaccgccg cgtcgcgcac gccgcgcagc tcggccagcg
     6601 ccgcctcgac ctcggggatc tcgacgcggt tgccgcggat cttcacctgg tggtccttgc
     6661 ggccggcatg gatcagctgt ccgtcgtcgc ggacgtaccc cagatcgccg gtcaggtaga
     6721 cccgccgcgt cgggtcggca gggtcgggaa cgaagacacg ctcgttgcgc tccggccgcc
     6781 gccaatagcc gggcgagagg taccggctgc gcacggcgat ctcgccgacg ctgccacgcg
     6841 gcagctcgca cgcccgctcg tcgacgatca gcacctcgac gccgtcgacg ggatggccgc
     6901 acggcacgcg caggtcgtcg acggtcgacc ggtggtcgag gaagaactgg cggatcgcgc
     6961 cgacctccgt cgccgcgagt ccgcaagaca acaacgtgcc cggctcgaag cagcggcgga
     7021 acgcctcgaa gtcggatcgg aagacgacct cgccgccgag cttgatcagc cgcaggtcgg
     7081 tgaagcgctc gccgcccaac gtccgcacga actggcggaa cagcgtcgcg acggagcagt
     7141 agatcgagat ccgccgcgcg acgatccacc ggccgagcgg tgccagaccg tcgagctcga
     7201 gcggatagcg gcag
//
LOCUS       DASPGN010000216        15945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1441933, whole genome shotgun sequence.
ACCESSION   DASPGN010000216 DASPGN010000000
VERSION     DASPGN010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 15945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15945
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441933"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..477
                     /locus_tag="VD788_06170"
     CDS             76..477
                     /locus_tag="VD788_06170"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HXV75890.1"
                     /translation="MRWRPADHLDRQLGVLWAAVAALSVLCYPLGRAVATVVPACPLR
                     TLAGVACPTCGSTRAILALGDGRLGEALAWNPLVTSAACLFLAGGLVAPWWNLGVGRL
                     PRTPDLARPVPRIVAIGILLVNWVWLVARGV"
     gene            complement(497..2929)
                     /locus_tag="VD788_06175"
     CDS             complement(497..2929)
                     /locus_tag="VD788_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HXV75891.1"
                     /translation="MIPRPLLPVAAVVAVLLIAAVGVMGTADGRAVTYRLRVAVTPTD
                     TDRLAALDLDVAGRDAAAGWVEVITGPDGLARLERAGFATSVIERRDGARPLAATEAG
                     RAVGAGYTDPGELELFLQAVHADHPAITRLEVIGAGIEGRPIYAMLISDNAAADEDEP
                     SLLFSGLHHAREVMTVEVMVDLIDRLTDGYDTDPAMKARVDAYQIWCVPLVNPDGLSY
                     VFDVDDLWRKNLRQNDNSGRISWKDGVDINRNYVWAWGNQCLGSSGTISSDLYRGAYP
                     GSEPETQALIALGRRIRPVFYVEYHSYGEDVFYAMGCDPVLSPVLSTIGGPDPSISRV
                     IAEQYAARIVQADGEPGYSPAPYGSRVDGVGRDHHAHEHGTIAFVTELNSAEEGAFQP
                     DYALWRDATVEGQRPGWTWLIDRVGGAAVAGHVVDAVDSSPLAAEIGLDQLDHPDGRR
                     LTSDPSSGRFHLIVVPGQYTLRVRAGGYVDAEIPLEVGDGWQPIVVALERDGSSLLYR
                     STFEHPEEIAGWSFVSPDDDASAGVWEWDAPFGTHDGDHPSATLRIGAPSFDRTAGEG
                     TRAFVTDNEPGAAIDDADVDGGATSLVSPEWDPNGRYGLTLRWQQWLRTDSADPLDGL
                     AVELSNDGGQSWHPAGWWGDEPSPDGPSARWQEQSLLLDTIAELGPSIRVRFRAIDAG
                     ADHVVEAAIDEVELRGYSLAGQGGVSGLTWAGGRTRLEWSPVPGGGQAGYQVARGALD
                     ALAAAADEIALGVIDCFDTAPGETFLDDADSPAPGAGWFYVVRFRLGFSIGDWGSGTG
                     GLPRIAEPDCAP"
     gene            complement(2943..3389)
                     /locus_tag="VD788_06180"
     CDS             complement(2943..3389)
                     /locus_tag="VD788_06180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV75892.1"
                     /translation="MSKDAPRRCEFFSDELNLELDTTIAADLREIDGVVERVLAVGAS
                     MNCGVGKEFEIETALREALANAVVHGAKHDRREKIQIAVACDERRGMLIVVRDPGGGF
                     DPESVPSPTHGQNLYRSHGRGIYLINELMDEVRFERGGAEIWMRKH"
     gene            complement(3743..5575)
                     /locus_tag="VD788_06185"
     CDS             complement(3743..5575)
                     /locus_tag="VD788_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXV75893.1"
                     /translation="MSYQPIENYGVIGDLHSIALVGLDGSIDFMCFPEFDSPTIFASL
                     LDDDRGGRFSLSPMLDDVRHKQLYLPDSCILLTRFLSADGVAELSDFMPVEQRHHAHD
                     LVRRMKTVRGELRYRMLCQPRFDYARASHGVERKDGEVIFASRGPDRTVIRLRSSVPV
                     EIVDGAAVAEFTLRAGETASFVLEQVIAGDESPSTAADYVSRGFRETIDFWHRWVRGM
                     RYDGRWREMLKRSAMTLKLLTSARHGSIVAAPTFGLPEQLSGERNWDYRYTWIRDASF
                     TLYALIRLGYTAEAGAFMSWIEARCGELGPDGSLQIMYGIDGRHDLTEHELGHLEGYR
                     GSKPVRIGNGAYAQLQLDIYGELMDSVYLYNKYGEPISFDLWRNLVRLIDWVCDNWRR
                     PDEGIWEVRGGRREFLYSKVLSWVAIDRGVRLAMKRSFPAPLARWLEVRDRIYLDVMR
                     NYWDSQLGAFVQYRGSAALDASSLLMPLVKFISPTDPRWLSTLRAIETRLVDDSLVYR
                     YRGPNAADDGLAGHEGTFNMCSFWYVECLSRAGDLDAARLCMEKMFGYANHVGLYGEE
                     LGPAGETRGNYPQAFTHLGLISAAYNLNEKLDLRRRGVPSPRRD"
     gene            complement(5667..6275)
                     /locus_tag="VD788_06190"
     CDS             complement(5667..6275)
                     /locus_tag="VD788_06190"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HXV75894.1"
                     /translation="MAHELPPLPYAFDALEPQIDARTMEIHHGKHHAAYVAKLNGAIE
                     GNAALERKSITELIGDLSSVPETIRAAVRNNGGGHFNHTLFWKVMKPGGGGEPGGELA
                     EAIRSTFGSFAEFKKKFSDAAANQFGSGWSWLFVKNGKLEVKGTPNQDSPVMDGGKPI
                     LGLDVWEHAYYLHYQNRRPDYVAAWWSVVNWNAVADNFKQAR"
     gene            complement(6325..7581)
                     /locus_tag="VD788_06195"
     CDS             complement(6325..7581)
                     /locus_tag="VD788_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75895.1"
                     /translation="MSSLAGRWTRVVFCAAALCQAPVDPRASTDVAAMTAALSAEVEQ
                     IRRLEFLRPVPLREIGDREAREHVLARLAKFQSGDEIAALERVYAILGLIPADGELLD
                     PLLEVLGQQAGGFYDPDSDSFYLRDDVPPTIAPVVITHELTHALEDQHFDIDRRLVRD
                     RGDDDRLLAHSAVHEGSAMLVMTIWIERATSRGEFDPAIAGRVSPDRVASAAGLDTLP
                     EVLRRQLLAPYILGPAFIAGGRPAGMPAAVFPAESVDALYRDPPQSSEQILHPHKYWD
                     AARRDEPREVELGRAGRALGRKWRLRHRGTLGELTLALLAGSPPTGDDGRIEWTNDPA
                     SGWGGDAWELWHGGGSAAVLLGTRWDTVADAREFADALAARGGLAWRRAGDRLAIAAG
                     AVEDRADEVLERMLAEMKTGALVPSE"
     gene            7853..8056
                     /locus_tag="VD788_06200"
     CDS             7853..8056
                     /locus_tag="VD788_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75896.1"
                     /translation="MNALLYKVYCYRGSDKQIWFEVEDTSTGQGVAWSPSRTTVVSKA
                     RKLGYSIDDDGRAVIKFYRAQAS"
     gene            8073..8855
                     /locus_tag="VD788_06205"
     CDS             8073..8855
                     /locus_tag="VD788_06205"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV75897.1"
                     /translation="MAAVVPVADPTFLSWLQALAQRHLSNLSFAEVRRGIQALSAWYV
                     ERRDRARPGIAFDGRGKRAAFALFYTPLHYLTVAGIVEATGLAAERPTGILDLGCGTG
                     PAGAAWSRGFSGGPVGVVGADTSGWTAGEARWNWARLGVPGTTRRSDLTRVRIGRRRA
                     VVLAWVVNELSAGDRVRLAGTLVAAIERGASVLVVEPIARSVVPWWDEWTRLFERAGG
                     RADTWRFACELPEPLRSLDRAAALDHRELTARSLCVQSRPSS"
     gene            complement(8834..10516)
                     /locus_tag="VD788_06210"
     CDS             complement(8834..10516)
                     /locus_tag="VD788_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75898.1"
                     /translation="MSRRTCERGWPVATGLLLLALVGCESAPEPGGEPVEGERAERSE
                     IDKYTTVRLTADLSGLSENQRRMIGSLIEAGRAMDEAFWLQAYGDRRQLLDSIADPDL
                     RRFAEINYGPWDRLDANRPFVDGVGAKPAGANFYPADMTREELETAATAAADGGAALR
                     SLYTIVERDESGSLTAVPYSRAFAEQVGRAAAKLREAAALAEDPGFRRYLELRAEALL
                     TDDYRASDLAWMEMKDNVVDVVIGPIETYEDQLLGYKAAFETFVLLKDQRWSERLSRY
                     AALLPDLQRGLPVADRYKSEMPGSDADLNAYDALYYAGDCNAGSKTIAINLPNDEQVQ
                     LSRGTRRLQLKNAMRAKFDEILLPISGLLIAEDQRPHVTFDAFFANTMFHEVAHGLGI
                     KNTLDGSSTVRAALRDHASTLEEGKADILGLYMVSELHERGELGAEPLEDYYVTFLAG
                     IFRSVRFGASNAHGRANMIRFNYFSEQGAFSRAADGTYRADFERMREAIDSLSELLLT
                     LQGDGDYDAAASLVASHGVVGDELQADLARLSRAGIATDIVFEQGSQLLGLD"
     gene            10692..12725
                     /locus_tag="VD788_06215"
     CDS             10692..12725
                     /locus_tag="VD788_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HXV75899.1"
                     /translation="MHEIRPARLKPGVDADALGDAGEDPERPAGHGRIRGRALIFWDP
                     HDPGRKLDAIDTDQITPAQDCVSESLDALDERWKAGAFRHLMPDFRQRVHAGETFVVA
                     GDRFAIGSSREMSPAGLKAVAAEAGRELVVVCGHNLGDIFRRNALNLGLHVVQAPDAV
                     ADARDGDVFVFDPPSRELTNETRGRSYSPMPLTDKEEEIRRTGGIFSVGRHVFAESVR
                     VAPRIEWPDAETASRLTATEQIVWAHRVDRDAPVEPGKTLRVYADLLPASDGTAPFAI
                     HTFNQITGGGTIDPRQAAIANDHFVFTGKDADERQTAIGREFARLHGIAKPYYATPGD
                     GIFHFYFPEQGLVMPGQFIPGADSHSRAYGAYGAVGIGVGSTTLGFGWSTGWVFFTLA
                     KARRVVLTGELQPWVSGKDIVLKLLERWGSRQAQGMSVEFVDRNRQLPIAYRNTIANM
                     MAEAEAQNGIFAADETTYEWYRAKGMHRLPYPEIRPGVDAGYEIDETLDLADVTPMIA
                     KPFSPNNAHPAAEVAGERIEFDKAYIGSCTNGSYEDLLQAALVLRAARQAGHRRAATD
                     FVVFPGSGGVKRAIEHGDPRLDGQSIAAVLREAGARIRDSWCGPCFGQGPDALREGQV
                     AITTFNRNWQNRMGVGGLGYLASPAVVAASALVGYMAPPDELGLEWDAERFGV"
     gene            12813..14240
                     /locus_tag="VD788_06220"
     CDS             12813..14240
                     /locus_tag="VD788_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXV75900.1"
                     /translation="MQRLLAVLILLIGAAGPARAAPCRVADPEAPRVLVLALDGVPLR
                     SIEAARAEGAFEGWPPPLPLVSSFPSVTNVAFTAILHPFGVDQAGGYEVAHFNRDKNK
                     VVGGSPFGYEERLYAWRDAFDVTSRTFGSKLATYTTPKRASRHGLDRAEQALYELPDK
                     QLILAHVGATDALIHLRGDKAILRFLIEVDERLREVKRAHRARTGRELSVVLLSDHGN
                     SSIKIRKAGGLRKSLERAGLAVTKHIDQPGTVVAPTFGIVGYGALFMHRSDAATAGRA
                     IVGNATVDLAAWLDEPSRMVVLSDAGAATVRWVEDGRGGLRMSYRMETGDPLEQAAAL
                     ERLSRRGLLDAAGYATESAWFDETVASEYPDAMRRLIHSLVGTWVENYATVVFSLKPG
                     YAWGWTSAHVSSKLSGGRLEGTHGGLDAASTIGFFMADDPARNPNGPVRADRALAGFA
                     GYNDCLVVADDDAPGDHDPAVSGPR"
     gene            complement(14246..14926)
                     /locus_tag="VD788_06225"
     CDS             complement(14246..14926)
                     /locus_tag="VD788_06225"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXV75901.1"
                     /translation="MARPRRRKPQVLRVRVVHEDDALVVVDKPAGLLSIATARESRRT
                     AYAQVFARLERRSPRERPFVVHRLDREASGLLVLAKTPEVKRALQAQFHDHSAGRSYA
                     AVVHGLVAIEHATLRSYLRENAAHTTYETSPGRGKLAVTHVEVLRRNAARGRTLVRLE
                     LETGRKHQLRAQLAAWGHPIVGDPRYGGDKTARLALHATRLRFRHPHSGRLVEFDSPL
                     PEAVRLMA"
     gene            complement(14932..>15945)
                     /locus_tag="VD788_06230"
     CDS             complement(14932..>15945)
                     /locus_tag="VD788_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75902.1"
                     /translation="VLDGVERPEHELELLAGLLACIEAEPFRAPLLVALQADLATSGS
                     PPSRRIARLARLVDMIESRRNMLFQPIAACGLLGTQLAFAVEEWRAACRGGVERWLAA
                     VGEIEALASLSCYAFEHPDDPFPAIADDGPLFDAVRLGHPLLPAGGGVRNDLRLDRGL
                     QLLLVSGSNMSGKSTLLRSVGVATAMALAGAPVRAERLTLSPLAIGASMRVTDSLQEG
                     MSRFYAEIRRLRRILDIGEARPPLLFLLDEILHGTNSHDRRIGAGALIRRLIEQGAVG
                     LVTTHDLALVRVAEELAPHAANVHFEDRLEGERIVFDYRLRDGVVQRGNAVELMRAIG
                     LPV"
BASE COUNT         2392 a         5583 c         5507 g         2463 t
ORIGIN      
        1 ctgtgcatcg ggctggccgt cctgtcggcg gtgttgatgg gcatcggtct cggcatgcag
       61 ggttagcgag cggcgatgcg ctggcgcccg gccgaccacc tcgaccgtca gctcggcgtg
      121 ctgtgggcgg ccgtcgcggc gctgtccgtt ctctgctatc cgctcggtcg agccgtcgcg
      181 acggtggtgc cggcctgccc gctgcgcacg ttggccgggg tcgcctgccc gacctgcggg
      241 tcgacgcggg cgatcctcgc gctcggtgac gggaggctcg gcgaggcact cgcctggaac
      301 ccgctcgtga cctcggccgc ctgcctgttc ctcgccggcg gtctggtcgc gccgtggtgg
      361 aacctcggcg tgggccggct tccccgaacg ccggacctcg cgcgaccggt gccaaggatc
      421 gtcgcgatcg ggatcctgct cgtcaactgg gtctggctcg tcgcacgcgg cgtctagctg
      481 ccggcgcgga acgtcgtcac ggtgcgcagt cgggctcggc gatgcgcggc agcccgccgg
      541 tcccactgcc ccaatcaccg atgctgaagc ccaagcggaa gcggacgacg tagaaccacc
      601 ccgcaccggg cgcgggcgag tcggcatcgt cgaggaaggt ctcgcccggc gcggtgtcga
      661 agcagtcgat caccccgagt gcgatctcgt cggctgccgc ggccagcgcg tcgagcgcac
      721 cgcgcgccac ctgatagccg gcctgtccgc cgccgggaac cggactccac tcgagtcgtg
      781 tccgaccacc ggcccacgtc agaccgctca ctcccccctg gccggcgagc gagtagccgc
      841 gcagctcgac ctcgtcgatc gccgcctcga cgacgtggtc cgcaccggcg tcgatcgcgc
      901 ggaagcggac gcggatcgac ggtccgagct cggcgatcgt atcgagcaac aacgactgct
      961 cctgccagcg cgccgacggg ccgtctggag acggctcgtc accccaccag ccggcgggat
     1021 gccagctctg cccgccgtcg ttcgacagct cgacggccaa tccgtcgagc gggtcggcgc
     1081 tgtctgtgcg cagccattgc tgccagcgca acgtgagacc gtagcggccg ttcgggtccc
     1141 actccggcga gaccagcgag gtcgcgccgc cgtcgacgtc ggcgtcgtcg atcgcggcgc
     1201 ccggctcgtt gtcggtgacg aaggcccggg tcccctcgcc ggcggtgcgg tcgaacgacg
     1261 gcgcaccgat ccggagcgtc gccgacgggt ggtcgccgtc gtgcgtgccg aacggggcat
     1321 cccattccca gacgcccgcg ctcgcgtcgt cgtcgggcga gacgaacgac caccccgcga
     1381 tctcctccgg gtgctcgaac gtgctgcgat agagcaggct cgacccgtcc cgttcgagcg
     1441 cgacgacgat gggttgccag ccgtccccga cctcgagcgg aatctcggcg tcgacgtagc
     1501 cgccggcgcg gacccgcagc gtgtactgcc ccggcacgac gatcaggtgg aatcgaccgg
     1561 aggacgggtc gctggtcagt cggcgcccgt cgggatggtc gagctggtcg aggccgatct
     1621 cggccgcgag tgggctcgag tcgacggcat cgacgacgtg cccggcgacg gcggcgccac
     1681 cgacgcggtc gatcaaccac gtccagccgg ggcgctggcc ctcgaccgtc gcgtcgcgcc
     1741 acaacgcgta gtccggttgg aacgcgcctt cttcggcgct gttcagctcg gtcacgaagg
     1801 cgatcgttcc gtgctcgtgc gcgtggtggt cgcggccgac gccgtcgacg cgcgagccgt
     1861 acggcgcggg cgagtacccc ggctcgccgt cggcctgcac gatccgggcc gcgtactgct
     1921 cggcgatcac ccggctgatc gacggatcgg ggccaccgat cgtcgacagc accgggctca
     1981 gcacggggtc gcagcccatc gcgtagaaca cgtcctcgcc gtagctgtgg tattcgacgt
     2041 agaagaccgg acggatgcgg cggccgagcg cgatcaaggc ctgggtctcg ggctcggagc
     2101 cggggtaggc cccgcgatag aggtccgacg agatggtgcc ggaggagccg aggcactggt
     2161 tgccccaggc ccagacgtag ttgcggttga tgtcgacgcc gtccttccac gagatccgtc
     2221 cgctgttgtc gttctgccgg aggttcttcc gccacaagtc gtcgacgtcg aagacgtacg
     2281 aaaggccgtc ggggttgacc agcggcacgc accagatttg ataggcgtcg acgcgcgcct
     2341 tcatcgccgg atcggtgtcg tatccgtcgg tcagccggtc gatgagatcg accatcacct
     2401 cgacggtcat cacctcgcgc gcgtggtgca gcccgctgaa cagcagcgac ggctcgtcct
     2461 cgtcggccgc cgcgttgtcc gagatcagca tcgcgtagat cggccggccc tcgatgccgg
     2521 cgccgatcac ctcgagccgc gtgatcgccg gatggtcggc gtggacggcc tgcaggaaga
     2581 gctcgagctc gcccgggtcg gtgtagcccg ctccgacggc ccgtccggcc tcggtcgccg
     2641 ccaacggtct ggccccgtcg cgccgctcga tcacgctggt cgcgaagccc gcgcgctcga
     2701 ggcgcgccaa cccgtcggga ccggtgatca cctcgaccca gcccgcggcc gcgtcgcgcc
     2761 ccgcgacatc gaggtcgagc gcggccaacc ggtcggtatc ggtcggcgtg accgctaccc
     2821 gcaaccggta ggtcaccgcg cgcccgtcgg ccgttcccat cacgccgacc gccgcgatca
     2881 gcagcacggc gacgacggcc gcgaccggca gcagcggccg cgggatcatc ggggccggcg
     2941 gctcagtgtt tgcgcatcca gatttccgca ccgccccgct cgaaacggac ctcgtccatc
     3001 agctcattga tcaggtagat gcctcgcccg tgcgagcggt agagattctg gccgtgcgtc
     3061 gggctcggca ccgattccgg gtcgaagccg cccccggggt cgcgtacgac gatcagcatg
     3121 ccgcgtcgct cgtcgcaggc cacggcgatc tggatcttct cgcgccggtc gtgcttggcc
     3181 ccgtgcacga cggcgttggc cagcgcctcg cgcagggccg tttcgatctc gaactccttt
     3241 ccgactccgc agttcatcga cgcgccgacc gcgagcacac gctcgacgac cccgtcgatc
     3301 tcccgaaggt ccgccgcgat cgtcgtgtcg agctccaggt tcagctcgtc cgagaagaac
     3361 tcgcatcgcc gcggagcgtc tttcgacatg tcgaggcccc cccgacccga ttttgcggta
     3421 agtatacggg gaggttgcca cgcgtggggt cccgagtcga gcgatccggg acacgggcgg
     3481 ggtagccggc gaccaggttc gagacggtcc ggccgcaatt tgcaaccgtg ctcgaggggt
     3541 ctggttccag aacgcggccg cggtcggccg ctcgaacctg ggcgccttcg gaccttcggt
     3601 ccagctgttc atacgcacgg gatcgaactc ggttctgaag ttctcgtttt ctgtcacggt
     3661 tgtcgcgggc gcgttcgtcg accgccggcc ggtgcgccac gcggatccgg tcgttcgcac
     3721 cgacagggcg ggccgcggtc gatcagtccc ggcgcggcga gggcactccg cgtcgcctga
     3781 gatccagctt ctcgttgagg ttgtacgccg cgctgatcag accgaggtgg gtgaacgcct
     3841 gcgggtagtt gccgcgcgtc tcgccggccg gtcccagctc ttcgccgtag agcccgacgt
     3901 ggttggcgta gccgaacatc ttctccatgc agagccgcgc ggcgtccagg tcgccggcgc
     3961 gcgacaggca ctcgacgtac cagaacgagc acatgttgaa cgtcccctcg tggccggcca
     4021 ggccgtcgtc ggccgcattc ggtccgcgat aacgatagac cagcgaatcg tccaccagcc
     4081 gcgtttcgat cgcacgtagc gtcgacagcc agcgggggtc ggtcgggctg atgaacttga
     4141 cgagcggcat caacaacgac gaggcgtcga gcgctgccga gccccgatac tgcacgaagg
     4201 cgccgagctg ggagtcccag tagttgcgca tcacgtcgag gtagatccgg tcgcgcacct
     4261 cgagccaacg cgcgagcggc gccgggaacg accgcttcat cgccagtctc acgccgcgat
     4321 cgatggcgac ccacgacaac accttggagt agaggaactc gcgacgcccg ccgcggacct
     4381 cccagatccc ctcgtcgggg cgccgccagt tgtcgcagac ccagtcgatc aaccgcacca
     4441 ggttgcgcca caggtcgaac gaaatcggtt cgccgtactt gttgtagagg tagaccgagt
     4501 ccatcagctc gccgtagatg tcgagctgaa gctgcgcgta cgctccgttg ccgatgcgca
     4561 ccggcttgga gccgcggtat ccctcgaggt ggccgagctc gtgctcggtc aggtcgtgcc
     4621 gcccgtcgat gccgtacatg atctgcagtg agccgtcggg tcccagctcg ccgcagcgcg
     4681 cctcgatcca actcatgaac gcgccggcct ctgcggtgta gcccaggcgg atcagcgcgt
     4741 acagcgtgaa cgaggcgtcg cggatccagg tgtagcggta gtcccaattg cgctctcccg
     4801 agagctgttc gggaaggccg aaggtcgggg cggcgacgat cgagccgtgc ctggccgagg
     4861 tcagaagctt gagcgtcatc gccgaacgct tcaacatctc gcgccagcgt ccgtcgtacc
     4921 gcatgccgcg cacccagcgg tgccagaaat cgatcgtctc gcggaaaccc cgcgagacgt
     4981 agtcggccgc ggtggacggc gactcgtcgc cggcgatcac ctgctcgagc acgaacgacg
     5041 ccgtctcgcc ggctcgcaag gtgaactcgg cgacggccgc accgtcgacg atctcgaccg
     5101 gaacgctcga tcgcaggcgg atgacggtcc gatcgggtcc gcgcgaggcg aagatcacct
     5161 cgccgtcctt tcgctcgacg ccatgcgacg cgcgggcgta gtcgaaccgt ggctggcaga
     5221 gcatgcgata acgcagctcg ccgcgaaccg tcttcatccg ccgcaccaga tcgtgcgcgt
     5281 ggtggcgctg ctcgaccggc atgaagtccg aaagctcggc cacgccgtcg gccgacagga
     5341 aacgcgtcag caggatgcag ctgtcaggca ggtacagctg cttgtgacgg acgtcgtcca
     5401 gcatcggcga caggctgaag cggccgccgc ggtcgtcgtc gagcagggag gcgaagatcg
     5461 tcggcgagtc gaactccgga aagcacatga aatcgatcga gccgtcgaga ccgaccaagg
     5521 cgatcgagtg caggtcgccg atcacgccgt agttctcgat cggctggtag ctcatcgtcg
     5581 ctccggtgag gatcgctcgg agacggaccg cggggtgtcc cgggcacgcg ggtcacccgg
     5641 gaaacacccc cgtcgtgtgc gatcggtcag cgcgcctgtt tgaagttgtc ggcgaccgcg
     5701 ttccagttga ccacactcca ccaggccgcg acgtagtccg gccggcggtt ctggtagtgc
     5761 aggtagtacg cgtgctccca gacgtcgagg ccgaggattg gcttgccgcc gtccatcacc
     5821 ggcgaatcct ggttgggcgt gcctttgacc tcgagcttcc cgttcttgac gaacagccaa
     5881 gaccaaccgg agccgaactg gttggctgcc gcgtcgctga acttcttctt gaactcggcg
     5941 aacgagccga aggtggacct gatcgcctcg gccagctcgc cgccgggctc gccgccgccc
     6001 cccggtttca tcaccttcca gaacagcgtg tgattgaaat gaccgccgcc gttgttgcgc
     6061 acggccgcgc ggatcgtttc cggaaccgag ctcagatcgc cgatcaactc ggtgatcgac
     6121 ttgcgctcga gggcggcgtt gccttcgatc gcgccgttca gcttggcgac gtaggccgcg
     6181 tggtgtttgc cgtggtggat ctccatcgtg cgcgcgtcga tctgcggttc gagcgcgtcg
     6241 aatgcatagg gcagcggtgg cagttcgtga gccatggcgt cctccttctg ctgactcgga
     6301 tgaatgacca acaatctatc gggttcactc gctcggcaca agcgcgccgg ttttcatctc
     6361 ggcgagcatc cgctcgagca cttcgtcggc gcggtcttcg accgcacccg ccgcgatcgc
     6421 cagccgatcg ccggcgcgac gccaggcgag accgccgcgc gcggcgagcg cgtcggcgaa
     6481 ctcccgggca tcggcgaccg tatcccagcg cgtgccgagc agcacggcgg cggagccgcc
     6541 gccgtgccac agttcccagg cgtctccgcc ccagccgctg gcggggtcgt tcgtccactc
     6601 gatccgcccg tcgtcgccgg tcggcggcga gccggccaga agcgcgaggg tcagctcgcc
     6661 gagcgtgccg cggtgacgaa gacgccactt gcggccgagc gcccggcccg cccggcccag
     6721 ctcgacctcg cgcggctcgt cgcgtcgggc cgcgtcccag tacttgtgag ggtggagaat
     6781 ctgctcgctc gattgcggcg gatcgcgata gagcgcgtcg accgattccg ccggaaagac
     6841 cgcggccggc ataccggcgg ggcgaccgcc ggcgatgaac gccggcccca ggatgtacgg
     6901 cgcgagcaac tgccgccgca gaacctcggg cagtgtgtcg agccccgcgg cgctcgcgac
     6961 tcggtcgggc gagacccgcc cggcgatcgc cgggtcgaac tcgccgcgcg acgtcgcgcg
     7021 ctcgatccag atcgtcatca ccagcatcgc cgatccctca tggacggcgg agtgggccag
     7081 caaccgatcg tcgtcgccgc gatcgcgcac gagccgccgg tcgatgtcga agtgttggtc
     7141 ttcgagcgca tgcgtcagct cgtgcgtgat gacgaccggg gcgatcgtcg gtggaacgtc
     7201 gtcacgcagg tagaacgagt cgctgtcggg gtcgtaaaac ccgcccgcct gctggcccag
     7261 cacctcgagc aggggatcga gcagctcgcc gtcggccggg atcaagccga ggatggcgta
     7321 gacccgctcg agcgcggcga tctcgtcgcc cgactggaat ttcgccagcc gtgcgagcac
     7381 gtgttcccgt gcctcgcggt cgccgatctc gcgcagcggg acggggcgca ggaactcgag
     7441 ccgtcggatc tgctcgacct cggcgctcaa tgcggcggtc atggctgcga cgtcggtcga
     7501 agctcgcgga tcgaccggcg cttggcacag cgcggcggcg cagaagacga ctcgggtcca
     7561 gcgaccggcc agagacgaca cgcttcctcc cgtcgaccgt ccgttcgccg ccagtctatc
     7621 cgaccggtcg atctcggggc gcgcaacggt tttccggaac gtcggcgtcg cacgacgggg
     7681 acccggaaaa caccggcgat gccgcgtttg cacgaccgcc tcggggccgg cgacgattcc
     7741 gaatgatgca aggcgttcgc gccccgtcga cccctccttt ttgcgatcga aaccgcttgt
     7801 tgcgccgtcg ccttctgcgt aactaatggg ggtcccccac atggagatcc cgatgaacgc
     7861 gctgctctac aaggtctact gctaccgtgg gtccgacaag cagatctggt tcgaggtcga
     7921 agacacgtcg accggtcagg gggttgcctg gtcaccgtcc aggacgacgg tcgtgagcaa
     7981 ggcgcgcaag ctcgggtaca gcatcgacga cgatgggcgc gcggtgatca agttctaccg
     8041 agcccaggcc agttgagccg agccgggcta ggatggccgc cgtggtaccg gtggccgacc
     8101 cgaccttcct ctcctggctt caggccctgg cgcaacgtca tctgtcgaac ctctccttcg
     8161 ccgaggtccg tcgcggaatc caggcgctgt ccgcgtggta cgtcgagcgg cgcgaccggg
     8221 cgcggcccgg gatcgccttc gacggccgcg gcaagcgcgc cgcgttcgcg ctgttctaca
     8281 cgccgctgca ttacctgacc gtcgccggca tcgtcgaggc gacggggctc gccgccgaac
     8341 ggccgaccgg catcctcgat ctcggttgcg gaaccggtcc ggcgggggcc gcctggtcga
     8401 ggggattctc gggcggccca gtcggcgtgg tcggcgcgga cacgagcggt tggaccgcgg
     8461 gcgaggcgcg ctggaactgg gcccggttgg gcgttcccgg cacgacgcgg cgaagcgacc
     8521 tgacgcgcgt gcggatcggc cgacgccggg ccgtcgtcct cgcgtgggtc gtcaacgagc
     8581 tgtcggccgg cgatcgggtc cgcctcgccg gcacgttggt cgccgcgatc gagcgcggtg
     8641 cgagcgtgct cgtcgtcgag ccgatcgcgc gcagcgtggt gccgtggtgg gacgagtgga
     8701 cgcggctgtt cgagcgggcc ggtggacggg ccgacacctg gcgcttcgcc tgcgagcttc
     8761 ccgagccgct ccgatcgctc gaccgcgccg cggcgctcga tcaccgcgag ctgacggcgc
     8821 gcagcctctg tgttcagtcg aggccgagta gctgagaccc ctgctcgaag acgatatcgg
     8881 ttgcgatgcc cgctcgactg agccgcgcca agtcggcctg cagctcgtcg ccgacgacgc
     8941 cgtggctcgc gaccaggctc gccgcggcgt cgtagtcgcc gtcaccttgc agcgtcagca
     9001 atagctcgga caacgagtcg atcgcctcgc gcatccgctc gaagtcggcc cggtaggtcc
     9061 cgtccgcggc tcggctgaac gcgccctgct cgctgaagta gttgaaacgg atcatgttcg
     9121 cccggccatg ggcgttcgac gcgccgaatc gcaccgagcg gaagatgccg gcgaggaagg
     9181 tgacgtagta gtcttcgagc ggctcggccc ccagctcgcc gcgctcgtgc agctcgctga
     9241 ccatgtacaa accgaggatg tcggccttgc cctcttcgag cgtcgaggcg tgatcgcgta
     9301 gcgcggcgcg cacggtcgag ctgccgtcga gcgtgttctt gatgccgagg ccgtgagcca
     9361 cctcgtggaa catcgtgttg gcgaagaacg catcgaaggt cacgtgcgga cgttggtcct
     9421 cggcgatcag cagaccggag atcggcagca ggatctcgtc gaacttggca cgcatcgcgt
     9481 tcttcagctg caggcgtcgc gtgccgcggg acagctgcac ctgctcgtcg ttcggcagat
     9541 tgatcgcgat cgtcttcgaa ccggcgttgc agtcgccggc gtagtacagc gcgtcgtagg
     9601 cgttcaggtc ggcgtcggat cccggcatct ccgacttgta tcggtcggcc accggaagac
     9661 cgcgctgcag gtcgggcagc agcgccgcat agcgcgagag acgctcgctc caccgctggt
     9721 ccttgagcag cacgaacgtc tcgaacgcgg ccttgtagcc gagcagctgg tcctcgtagg
     9781 tctcgatcgg accgatgacg acgtcgacga cgttgtcctt catctccatc cacgccaggt
     9841 ccgacgcccg gtaatcgtcg gtcagcaacg cctcggcccg tagctcgagg tagcggcgaa
     9901 agccggggtc ctcggccagc gccgccgcct cgcgcagctt cgccgcggcg cggccgacct
     9961 gctcggcgaa tgcccggctg tagggcaccg ccgtgagcga cccgctctcg tcgcgctcga
    10021 cgatcgtata caggctgcgt agcgccgccc cgccgtcggc cgcggcggtc gccgcggtct
    10081 cgagctcctc gcgggtcatg tccgccggat agaagttggc gcccgcgggc ttggcgccga
    10141 caccgtcgac gaacggccgg ttggcgtcca accggtccca cggtccgtag ttgatctcgg
    10201 cgaatcgtct caggtcgggg tcggcgatcg aatcgagcaa ctgccggcgg tcgccgtagg
    10261 cctgcagcca gaacgcctcg tccatcgcgc gaccggcctc gatcaacgag ccgatcatcc
    10321 gccgctggtt ctcgctcagg ccactcaggt cggcggtcag ccgcaccgtc gtgtacttgt
    10381 cgatctcact cctctccgcg cgctcgccct cgaccggctc accgccgggc tccggcgcgg
    10441 actcgcaccc cacgagcgcc aggagcagca gtcccgtcgc caccggccat cccctttcgc
    10501 acgttcgccg actcatcgat cttcgcacct ccgccgacgc atcttatctc ggatcgcgcc
    10561 gcgcttgaca ggtcgaggac atcgtgcatc atcgtcagca cgggagcctc atcggacaac
    10621 gagaacgagt ctgacggcat cgcgccccgg atggcgcgtg gcgtgggccg gcaggccgag
    10681 gagttccgtc gatgcacgag atcaggccgg cccggctcaa gccgggcgtg gacgccgacg
    10741 cgctcggcga cgccggcgag gatccggagc ggcccgccgg gcacgggcgg atccgcggtc
    10801 gcgccttgat cttctgggac ccgcacgacc cggggcgcaa actggacgcg atcgacaccg
    10861 atcagatcac cccggcgcag gattgcgtat cggaaagcct ggacgcgctc gacgagcgct
    10921 ggaaggccgg agcgttccgc cacctgatgc ccgactttcg ccagcgggtt cacgcgggcg
    10981 agacgttcgt cgtcgccggc gaccgtttcg cgatcggctc ctcgcgcgag atgagcccgg
    11041 ccggcctgaa ggcggtcgcc gcggaggccg gacgcgagtt ggtcgtggtg tgcggccaca
    11101 acctgggcga catcttccgc cgcaacgcgc tgaacctcgg cttgcacgtc gtccaggcac
    11161 ccgatgcggt ggcggacgcc cgtgacggcg acgtcttcgt tttcgatccg ccatcccgcg
    11221 agctgaccaa cgaaacgcgc ggccgcagct actcgccgat gccgctgacc gacaaggaag
    11281 aggagatccg gcggaccggc ggcatcttct cggtcgggcg ccacgtcttc gccgaatcgg
    11341 tgcgcgtcgc tccgcgcatc gagtggcccg acgccgaaac cgcctcgcgc ctgaccgcga
    11401 ccgagcagat cgtctgggcc catcgcgtcg accgtgacgc gccggtcgag ccgggcaaga
    11461 cgctgcgcgt ctacgccgac ctgttgccgg cgtcggacgg caccgcgccg ttcgcgatcc
    11521 acaccttcaa ccagatcacg ggcgggggca cgatcgatcc gcgccaggcg gcgatcgcca
    11581 acgaccactt cgtcttcacc ggcaaggacg cggacgaacg gcagaccgcg atcggacgcg
    11641 agttcgcgcg cctacacggc atcgccaagc cgtactacgc gacgccgggc gacggcatct
    11701 tccatttcta cttcccggag cagggactcg tcatgcccgg tcagttcatc ccgggcgccg
    11761 attcgcacag ccgtgcctac ggtgcctatg gcgcggtcgg tatcggcgtc ggatcgacga
    11821 cgctcggctt cggttggtcg acgggctggg tcttcttcac gctggccaag gcccgtcgcg
    11881 tggtgctgac cggcgagttg caaccgtggg tctccggcaa ggacatcgtg ctgaagctgc
    11941 tcgagcgctg gggctcgcgc caggcgcagg gaatgtcggt cgagttcgtc gaccgaaacc
    12001 gccagctgcc gatcgcctat cgcaacacga tcgccaacat gatggccgag gccgaggcgc
    12061 agaacggcat cttcgccgcc gacgaaacga cctacgagtg gtaccgcgcc aagggcatgc
    12121 atcggctgcc ctaccccgag atccgaccgg gggtcgatgc gggttacgag atcgacgaga
    12181 cgctcgatct ggccgacgtg acaccgatga tcgccaagcc gttcagcccg aacaacgccc
    12241 acccggcggc cgaggtggcc ggcgaacgga tcgagttcga caaggcctac atcggctcgt
    12301 gcaccaacgg cagctacgaa gatctgttgc aggccgcgct cgtgctgcgc gcggcgcgtc
    12361 aggccggtca ccggcgcgcc gccaccgact tcgtcgtgtt ccccggatcg ggtggggtca
    12421 agcgggcgat cgagcacggc gatccgaggc tcgacgggca gtcgatcgcc gccgtgctgc
    12481 gcgaagcggg ggcacggatc cgggactcgt ggtgcggccc gtgtttcggc caagggccgg
    12541 acgcgctgcg cgagggccag gtcgcgatca ccacattcaa ccgcaactgg cagaacagga
    12601 tgggcgtcgg cggtctggga tacctcgcca gccccgcggt cgtcgccgcc tcggcgctgg
    12661 tcggctacat ggccccaccc gacgagctgg gtctcgagtg ggacgccgag cggttcggcg
    12721 tctgaccgcg ggatccggcg cggactgcga cggggatcgc ggtgatgtaa catctgcgac
    12781 gttcgcccgt tatcggtttc gacgaggcac cgttgcaacg actgctggcc gtgttgatcc
    12841 tgttgatcgg cgccgcggga ccagcgcgcg cggcgccgtg ccgcgtcgcg gacccggaag
    12901 cgccgcgcgt gctggtgctg gcgctcgacg gggtgccgct gcggtcgatc gaggcggcgc
    12961 gcgccgaggg ggcgttcgaa gggtggccgc cccccctgcc gctggtctcg tccttcccgt
    13021 cggtgaccaa cgtcgcgttc accgcgatcc tgcatccgtt cggcgtcgat caggccggcg
    13081 gctacgaggt cgcccacttc aaccgtgaca agaacaaggt cgtcggcggc tcgccgttcg
    13141 gctacgagga acggctgtac gcctggcgcg acgcgttcga cgtcaccagc cggaccttcg
    13201 gctcgaagct cgcgacctac accacgccga aacgcgcctc gcggcacggc ctggacaggg
    13261 ccgagcaggc gctgtacgaa ctgcccgaca aacagctgat cctggcgcac gtcggcgcga
    13321 cggacgcgct gatccacctg cgcggcgaca aggcgatcct ccgtttcttg atcgaggtcg
    13381 acgagcgact gcgcgaagtc aagcgagccc atcgggcccg caccggtcgc gagctgagtg
    13441 tcgtgctgct gtcggaccac ggcaacagct cgatcaagat ccgcaaggcg ggtgggctgc
    13501 gcaagtcgct cgagcgcgcc ggtctcgcgg tgaccaagca tatcgatcaa ccggggacgg
    13561 tcgtcgcgcc gacgttcggt atcgtcggtt acggcgcgct gttcatgcac cgctccgatg
    13621 ccgccaccgc cggccgggcg atcgtcggca acgccaccgt cgacctggcg gcatggctcg
    13681 acgagccgag ccggatggtg gtgctgtcgg acgccggcgc cgccaccgtg cgctgggtcg
    13741 aggacggccg cggcgggctg cgcatgtcgt atcggatgga aaccggcgac ccgctcgagc
    13801 aagccgccgc gctcgaacgg ctgtcccggc gcggactgct cgatgccgcc ggctacgcga
    13861 ccgagagtgc ttggttcgac gagacggtcg cgagcgaata tccggacgcg atgcgccgct
    13921 tgatccactc gttggtcggc acctgggtcg agaactacgc gaccgtggtc ttttcgctca
    13981 aacccggcta cgcctggggc tggacctcgg cccacgtcag ctcgaagctg agcggcgggc
    14041 gtctcgaggg gacccacggc ggtctcgacg cggcgtcgac gatcggcttc ttcatggccg
    14101 acgatcccgc gcggaacccc aacggacccg tgcgggccga ccgcgcgctg gccggcttcg
    14161 ccggttacaa cgactgcctg gtggtcgccg acgacgacgc gcccggcgac cacgacccgg
    14221 cggtttccgg tccgcgctga ccgctctacg ccatcaggcg gaccgcctcg ggcagcggcg
    14281 agtcgaactc gacgagccgg ccgctgtgcg ggtggcggaa gcgcaaccgg gtcgcgtgga
    14341 gcgcgagacg cgccgtcttg tcgccgccgt agcgcgggtc gccgacgatc gggtgccccc
    14401 aggcggcgag ttgcgcgcga agctggtgct tgcggccggt ctcgagctcg agccgcacga
    14461 gcgtgcggcc gcgcgcggcg ttgcgccgca ggacctcgac gtgggtcacc gcgagcttgc
    14521 cgcggccggg actcgtctcg taggtcgtgt gcgccgcgtt ttcgcggagg tacgaacgca
    14581 gcgtcgcgtg ctcgatcgcg acgagcccgt ggacgaccgc cgcgtagctt cgcccggccg
    14641 agtggtcgtg gaactgcgcc tgcagcgcgc gcttgacctc gggggtcttg gccagcacga
    14701 gcaggcccga cgcctcgcga tcgagccgat ggacgacgaa cggccgctcg cgcgggctgc
    14761 gacgctcgag ccgggcgaac acctgggcgt acgccgtgcg tctcgactcc cgcgcggtcg
    14821 cgatcgaaag caggccagcc ggtttgtcga cgacgacgag cgcgtcgtcc tcgtggacca
    14881 cgcggacgcg aaggacttgc ggcttgcgtc gtcggggtcg tgccatccga ctcagaccgg
    14941 gagcccgatc gcccgcatca gctcgacggc gttgccccgc tgcaccaccc cgtcgcgcag
    15001 ccggtaatcg aagacgatcc gctcgccctc cagtcggtcc tcgaaatgca cgttggccgc
    15061 gtggggcgcc aactcctcgg cgacacggac cagcgccagg tcgtgcgtcg tgaccagtcc
    15121 gaccgcgccc tgctcgatca gccgtcggat cagcgcgccc gcaccgatgc gacgatcgtg
    15181 ggagttggtg ccgtgcagga tctcgtcgag cagaaacaag agcggcggcc gcgcctcgcc
    15241 gatgtcgagg attcgcctca gccggcggat ctcggcatag aaccgggaca tcccctcctg
    15301 caacgaatcc gtcacgcgca tcgacgcacc gatcgcgagc ggcgagaggg tcaatcgctc
    15361 ggcccgcacc ggcgcgccgg ccagcgccat cgccgtcgcc acgccgaccg agcgcaggag
    15421 cgtgctcttg cccgacatat tggagccgct gacgagcagc agctgcaacc cgcggtccaa
    15481 ccggagatcg ttgcgcacgc ctccgcccgc cggcaacaac ggatgcccca accgcaccgc
    15541 gtcgaacagc ggtccgtcgt cggcgatcgc ggggaacggg tcgtccgggt gctcgaaggc
    15601 gtagcacgaa agcgacgcca gcgcctcgat ctccccgaca gcggcgagcc agcgctcgac
    15661 gccgccgcgg catgccgcac gccactcttc gaccgcgaag gcgagctgcg taccgagcag
    15721 accgcatgcc gcgatcggct ggaacagcat gttgcggcgc gactcgatca tgtcgaccag
    15781 acgggcgaga cgggcgatgc ggcgcgacgg cggcgaaccc gacgtagcga ggtcggcctg
    15841 cagcgcgacg agcaacggcg cgcggaacgg ttcggcctcg atacacgcga gcagtccggc
    15901 cagcagctcg agctcgtgtt cgggtcgctc gacgccgtcg agcac
//
LOCUS       DASPGN010000217         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_505642, whole genome shotgun sequence.
ACCESSION   DASPGN010000217 DASPGN010000000
VERSION     DASPGN010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505642"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..930)
                     /locus_tag="VD788_06235"
     CDS             complement(4..930)
                     /locus_tag="VD788_06235"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXV75903.1"
                     /translation="MSIRPVKQITRARPTLEGAGVRLDRAFGFGKTQEFDPFLLLDDF
                     RNERREDYERGFPWHPHRGIETITYVLAGNVRHADSLGNAGTIGAGDVQWMTAGSGII
                     HQEMPQGESTGRMHGFQLWANLPAAKKMTAPRYQEIVAGEVPEVVEDDGTRARVICGA
                     RWGARGPVAGIAADPSYVDVSVPPGKTRRIPVATARHAFAYVFEGDGRFADASAPRGV
                     PTEVVDGVTGVAAEAAPERVGNRSLVLFDRGDEVVVHAGEHGIRFLLVSGQPLREPVA
                     WRGPIVMNTDAELRQAFAELDDGTFLERGRQR"
     gene            1017..>2067
                     /locus_tag="VD788_06240"
     CDS             1017..>2067
                     /locus_tag="VD788_06240"
                     /inference="COORDINATES: protein motif:HMM:NF021021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HXV75904.1"
                     /translation="MGMKLAALVSAWLLALPALAAPAHYVVFEVDAAGEAEPVFAARV
                     ELPARGDTVGAEAADAARARALAGEALRVLELRAPDGRVVFREVVALEHRLRGEFHGK
                     PHPAGGHEIEAHGALVERAAFVARVPALAGARLVVDGPTRAEFDLDRLAARAGRLPLA
                     HHTGGAAAVARGAAGDPTNRVDLVIMGDGYTAAESALFQAQAANLEDQFLSITPYAEY
                     RNFVNVTHLFTASAESGADHPPFRTQNCTGDDPSCCSDPVMRDDPLRNTFVDTAFDAR
                     YCAFQIHRLLVIDVSAVLAAASAVPEWDQILVLVNDTTYGGSGGFIGVSSTHPAAVDV
                     SRHEYGHSFTGLADEY"
BASE COUNT          261 a          775 c          738 g          293 t
ORIGIN      
        1 ccgctaccgc tgacggcccc gctcgaggaa ggtgccgtcg tcgagctcgg cgaacgcctg
       61 gcgcagctcg gcgtcggtgt tcatcacgat cggaccgcgc caggcgaccg gctcgcgcag
      121 cggttggccc gagacgagca ggaagcggat gccgtgctcg ccggcgtgca cgacgacctc
      181 gtcgccgcga tcgaacagca cgagcgagcg gttgccgacg cgctcgggcg ccgcctcagc
      241 ggcgaccccg gtcaccccgt cgacgacctc ggtcggcacg ccgcgcggcg ccgaggcatc
      301 ggcgaagcgg ccgtcgccct cgaaaacgta ggcgaaggcg tgacgtgcgg tcgcgaccgg
      361 gatgcgccgc gtcttgcccg gcggcaccga cacgtcgacg tagctcgggt cggcggcgat
      421 ccccgcgact gggccgcgcg cgccccaccg cgcgccgcag atcacgcggg cccgcgtgcc
      481 gtcgtcctcg accacctccg gcacctcgcc ggcgacgatc tcctgatagc gcggcgccgt
      541 catcttcttc gcggccggca ggttggccca cagctggaag ccgtgcatcc ggccggtcga
      601 ctcgccctgg ggcatctcct gatggatgat gccgctgccg gccgtcatcc actgcacgtc
      661 gccggcgccg atcgtgccgg cgttgcccag gctgtcggcg tggcgcacgt tgccggccag
      721 cacgtaggtg atcgtctcga tcccgcggtg cggatgccac gggaagccgc gctcgtagtc
      781 ctcgcggcgc tcgttgcgga aatcgtcgag cagcaggaac gggtcgaact cctgcgtctt
      841 gccgaaaccg aacgcgcggt cgagccgcac gccggcgccc tcgagcgtcg gacgggcgcg
      901 ggtgatctgc ttgacgggtc ggatcgacat ggcggcctcc tctctctcgg tcgtccgatt
      961 atccccgcga cccggggaag tcgccacgcc cggggccgat cgggagtagt agattgatgg
     1021 gtatgaagct cgcggcgctc gtttccgcct ggctgctcgc gttgccggcg ctcgccgcgc
     1081 cagcgcacta tgtcgtcttc gaggtcgacg cggcgggcga ggccgagccg gtcttcgccg
     1141 cgcgggtcga gctgcccgcg cgcggcgata cggtcggggc cgaggcggcc gacgcggcgc
     1201 gggcgcgcgc gctcgcgggc gaggcgctgc gcgtgctcga gctgcgcgcc cccgacggtc
     1261 gcgtcgtttt tcgcgaggtc gtcgcgctcg agcaccggct gcgcggcgag ttccacggca
     1321 agccgcaccc ggcgggcggc cacgagatcg aggcgcacgg cgcgctcgtc gagcgcgcgg
     1381 cgttcgtcgc ccgcgtgccc gccttggccg gcgcgcggct cgtcgtcgac ggcccgacgc
     1441 gcgccgaatt cgatctcgac cggctggcgg cgcgcgccgg gcgcctgccc ctcgcccatc
     1501 acacgggcgg cgccgcggcg gtcgcgcgcg gcgcggcggg cgacccgacg aaccgcgtcg
     1561 atctcgtgat catgggcgac ggctacaccg cggccgagtc cgcgctgttc caggcgcagg
     1621 ccgcgaacct cgaggaccag ttcctgtcga tcacgcccta tgccgagtac cgcaacttcg
     1681 tcaacgtcac gcacctgttc accgcgtcgg ccgagtcggg cgccgaccac ccgccctttc
     1741 gcacccagaa ctgcacgggc gacgacccga gctgctgcag cgacccggtg atgcgcgacg
     1801 acccgctgcg caacaccttc gtcgacaccg ccttcgacgc gcgctactgc gcgttccaga
     1861 tccaccggct gctcgtgatc gacgtctcgg cggtgctggc cgcggcgtcg gcggtgcccg
     1921 agtgggacca gatcctcgtg ctcgtcaacg acacgacata cggaggctcg ggcggcttca
     1981 tcggcgtcag ctcgacgcac ccggcggcgg tcgacgtctc acgccacgag tacggccact
     2041 cgttcaccgg tctggccgac gagtacg
//
LOCUS       DASPGN010000218         6307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1324688, whole genome shotgun sequence.
ACCESSION   DASPGN010000218 DASPGN010000000
VERSION     DASPGN010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324688"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VD788_06245"
     CDS             complement(<1..1164)
                     /locus_tag="VD788_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75905.1"
                     /translation="MSIGVFQRSAWRVVGCLIVIAGALATRAGQPQTLPVEGHREQTG
                     DPWAPVWRERQLRSTPARVARGNFVSVQVNVDAAGNNIVGDAANEPSIAIDPTVPANI
                     VIGWRQFDTVASNFRQAGYAYSRDAGQSWVFPGVLQPGQFRSDPVLAADAAGTFYYYS
                     LSTVTTVEMFVSNDKGASWLGPFPAFGGDKEWMIADLSTSPAAGQIFALWNSQFTCCA
                     PGTDFTRSSDGGFNYDGPFAMPVKVKWGTLDTADDGRLFVVGSDLNTTAVPSHYVMRS
                     TNAPDPGVAPVFELTTPVDLGGTTVAGGPPNPGGLLGQVWIAADRNSRPGYVYVLASV
                     NPPGSDPLDVRFVRSTNYGQSWSPSSRVNDLPPLATAYQWFAAMSVAPNGRIDV"
     gene            1318..3267
                     /gene="acs"
                     /locus_tag="VD788_06250"
     CDS             1318..3267
                     /gene="acs"
                     /locus_tag="VD788_06250"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXV75906.1"
                     /translation="MSNDLYPVKPAIRQRAHVNSLEEYQRMYRLSLDNPQWFWGEQAR
                     TLTWYHPWHSVFDADYDEVDFAWFSGGRLNACFNCVDRHLADNGDRTAIVWAGDEPGT
                     YRHISFRELKHHVCRLANVLISHGVRKGDRVCIYMPMIPETVYAMLACARIGAVHSVV
                     FGGFSADALRDRIVDAQCRVVITANEGLRGGKRIPLKRITDRAVEGMSTVETVLVARR
                     TDGDVSMTPGRDHWLDEEMRRQRSTCTTEWMGAEDPLFILYTSGSTGRPKGVLHTTGG
                     YLVYAATTHRYVFDYHAGDVYCCAADVGWITGHSYIVYGPLANGATTVMFESTPTHPD
                     AGRYWRMVDDLGINIFYTAPTALRAIAQAGDEFVRRYKRTSLRILGSVGEPINPEIWK
                     WYHDVVGEGRCAVVDTWWQTETGGILISPLPGVTATKPGSATLPLFGIKPVVMGADGK
                     PLDGNDVAGALCLASPWPGQARTVHGDHHRFKETYFSSYPGYYFTGDGCRRDADGYYW
                     ITGRIDDVLNVSGHRLGTAEVESALVAHEAVAEAAVVGFPHPIKGNGIYAYVVLGADF
                     DGQSKESIAGALKEQVRHAIGAFAAPDAIHVASGLPKTRSGKIMRRILRKIAASDYTG
                     LGDTTTLAEPEVVERLIAEHKARGG"
     gene            complement(3284..4615)
                     /locus_tag="VD788_06255"
     CDS             complement(3284..4615)
                     /locus_tag="VD788_06255"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HXV75907.1"
                     /translation="MQQERGQWGSSVGFVLAASGSAIGLGNIWRFPYVVGTHGGAAFV
                     LLYLACVLLICLPYLFAELAVGRRTAKNPVGAILALKPRSAWPVVGGLGVLTGVFILS
                     YYAVIAGWAFGYILKTLIAPDMPHAEFTASPAIVIPLFAWFIALTMLVVLGGIEHGIE
                     RWSKILMPVLLVLMLLVIARGLTLPGAGAGLEFYLKPDFSQVTGRTLLTALGQAFFSL
                     SLGMGAMVTYGSYLPRTADLQMSGLWVAVSDTLVALLAGFMIFPAVFAMGKNPAAGPT
                     LLFEVLPEIFAAMPFGIPVAIVFFVLLSIAALTSTVSLLEVVVAYFIDERRWSRRRAV
                     IAVGIFTFLVGLPSALSQGASSTLSTISLHGNASFLGVMDFIWGNLSLAIGALLLSVF
                     VGWVWGVAPAGAELREGSRVPPVTIAAWGLFIRYVCPVVIFVVLLNLFGLF"
     gene            4794..5549
                     /locus_tag="VD788_06260"
     CDS             4794..5549
                     /locus_tag="VD788_06260"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HXV75908.1"
                     /translation="MDKMKLLSSGLLLLLAGLPTFAQQGQGVDAAGRSPAAIEILQKV
                     DAAVKAISAVRYDVKTVPGGVAEQMVGPGEASVVLIGWNGRIPEKFHVEGKTARGGQP
                     VTVTGGGNGDSYFLVDHTTKKGYEDMDPAVLGGSAQALMGFAMLEFVHDRPFDDELAA
                     EVAELRGSKTIGGVDCHEIRVVYSSGQGESIWYFSKTDYTPRGRVRVFNTPQGEGTIT
                     VTIENLEIDPKVDATLFVMNLPAGYEEIDDFAP"
     gene            complement(5559..>6307)
                     /locus_tag="VD788_06265"
     CDS             complement(5559..>6307)
                     /locus_tag="VD788_06265"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV75909.1"
                     /translation="NHGYSSSPIEYGDTVIALVGGEGRSVVAFDKKTGETKWKAGDFK
                     NSYSTPRVLELAGREQLITFMADAVVGMDPADGAVLWSHPHQNQWEQNITMPIKIDDD
                     HLFISSPQAGAKALKLTDNGDGFVVEETWSTRKIQFYHVNTVRDGDYVYGSTGTMAPS
                     FMSALNLKTGEIAWRKRGYAKANCVSADGQLFILDEDGVLYLTTASPDDLVLHSKIDL
                     TEDVAWTVPTIAGKTMYVRDKSRIMALDLG"
BASE COUNT         1128 a         2133 c         2116 g          930 t
ORIGIN      
        1 cacgtcgatc cgtccgttgg gggcgaccga catcgcggcg aaccactgat aggccgtcgc
       61 gagcggcggg aggtcgttga cgcggctgga cggggaccac gactgaccgt agttcgtgct
      121 gcgcacgaag cgaacgtcga gcgggtcgct cccgggcggg ttgaccgacg ccagcacgta
      181 gacgtagccg gggcgcgagt tgcggtcggc ggcgatccac acctgtccga gcagcccgcc
      241 ggggttcggc ggaccgccgg cgaccgtcgt cccgccgagg tcgaccggcg tcgtaagctc
      301 gaacaccggc gcgacgcccg gatcgggggc gttggtcgag cgcatcacgt agtgcgaggg
      361 aacggccgtc gtgttgagat cggagccgac gacgaacagt cgcccgtcgt cggccgtgtc
      421 gagcgtgccc cacttgacct tgaccggcat ggcgaacggc ccgtcgtagt tgaacccgcc
      481 gtcgctcgat cgggtgaagt cggtgcccgg cgcgcagcag gtgaactgcg aattccacag
      541 cgcgaagatc tgaccggcgg ccgggctcgt gctgaggtcc gcgatcatcc actccttgtc
      601 gccgccgaag gccgggaacg gtccgagcca gctcgcgccc ttgtcgttcg agacgaacat
      661 ctcgaccgtg gtcaccgtgc tgaggctgta atagtagaag gtacccgccg cgtcggcggc
      721 gagcaccggg tcgctgcgga actggccggg ctgaagcacg cccggaaaga cccagctctg
      781 accggcgtcg cggctgtagg catagcccgc ctgacggaag ttggaagcga cggtgtcgaa
      841 ttgcctccag ccgatcacga tgttggccgg gaccgtcggg tcgatcgcga tcgacggctc
      901 gttggccgcg tcgccgacga tgttgttgcc ggcagcgtcg acgttgacct gcacgctgac
      961 gaagttgccg cgcgcgaccc gcgctggcgt ggagcgaagc tgccgttcgc gccacaccgg
     1021 cgcccacgga tcgccggttt gttcgcggtg cccctcgacg ggcagcgtct gcggctgacc
     1081 ggcccgggtg gcgagcgcac cggcgatcac gatcaggcag ccgacaacgc gccaggcgga
     1141 tcgctgaaag accccgatcg acatcggccc cccgcatcgt cctgcgacac gccacctcga
     1201 cggcaagaca gcgagcaatc tacgccaccg cactcggggc ggcaaccgga cggcgagcgt
     1261 ggtgcgtcgg acccgaattg gggtacgatc gccgaacgcc acgataggag atccaccgtg
     1321 tcgaacgacc tctatccggt caagcccgcg atccggcaac gggcccacgt caactcgctc
     1381 gaggagtacc agcgtatgta ccgcctctcg ctggacaacc cgcagtggtt ttggggcgag
     1441 caggcgcgca cgctgacctg gtaccacccg tggcactcgg tgttcgacgc cgactacgac
     1501 gaggtcgact tcgcctggtt ctccggtggc cggctgaacg cctgcttcaa ctgcgtcgat
     1561 cgtcacctgg ccgacaacgg cgaccgcacg gcgatcgtct gggccggcga cgagccgggc
     1621 acctaccgcc acatctcgtt ccgtgagctc aagcaccacg tctgtcgtct ggccaacgtg
     1681 ttgatcagcc acggcgtgcg caagggcgac cgcgtctgta tctacatgcc gatgattccg
     1741 gagaccgtct acgccatgct ggcctgtgcc cgcatcggtg cggtccactc cgtcgtcttc
     1801 ggcggtttca gcgccgacgc gcttcgcgat cggatcgtcg atgcacagtg tcgcgtcgtg
     1861 atcaccgcca acgaaggact acgcggcggc aagcgcatcc cgctcaaacg gatcaccgat
     1921 cgcgcggtcg agggtatgtc gaccgtcgag accgtgctgg tggcgcgacg caccgacggc
     1981 gacgtgtcga tgacgcccgg gcgcgatcac tggctggacg aggagatgcg gcggcagcgc
     2041 tcgacctgca ccacggaatg gatgggcgcg gaagacccgc tgttcatcct ctacacctcg
     2101 gggagcaccg ggcggccgaa gggcgtgctg cacacgaccg gcggctacct ggtctacgcc
     2161 gcgacgactc accgctacgt cttcgattac cacgcgggag acgtctactg ttgcgcggcc
     2221 gacgtcggct ggatcaccgg tcacagctac atcgtctacg ggccgctggc caacggggcg
     2281 acgacggtga tgttcgagtc gacgccgacc catccggacg ccgggcgcta ttggcggatg
     2341 gtcgacgacc tcggcatcaa catcttctac acggcaccga ccgcgctacg cgcgatcgcg
     2401 caggccggcg acgagttcgt tcgccgctac aagcgcacca gcctgcgcat cctgggctcg
     2461 gtcggcgaac cgatcaaccc cgagatctgg aagtggtatc acgacgtcgt cggcgaggga
     2521 cgctgcgccg tcgtcgacac ctggtggcag accgaaaccg gcggcatcct gatctcgccg
     2581 ctgcccggcg tgaccgcgac caaacccggc tcggcgaccc tgccgctgtt cggcatcaag
     2641 ccggtggtga tgggcgccga cggcaagccg ctcgacggca acgacgtcgc cggcgcgctg
     2701 tgtctggcct cgccctggcc gggccaggcg cgcacggtac acggcgacca tcaccgcttc
     2761 aaggaaacct atttctcgag ctaccccggc tactacttca ccggcgacgg ctgccgccgt
     2821 gacgccgacg gttactactg gatcaccgga cggatcgacg acgtgctcaa cgtctcggga
     2881 caccgcctcg gcacggccga ggtcgagagc gcgctggtgg cgcacgaggc ggtcgccgaa
     2941 gcggcggtcg tcggtttccc gcacccgatc aagggcaacg ggatctacgc ctacgtcgtc
     3001 ctcggcgcgg acttcgacgg ccagagcaag gagtcgatcg ccggcgcgtt gaaggagcag
     3061 gtgcgccacg cgatcggcgc gttcgccgcg cccgacgcga tccacgtcgc ctccgggctg
     3121 cccaagaccc gcagcggcaa gatcatgcgc cgtatcctgc gcaagatcgc cgcatccgac
     3181 tacaccggtc tgggcgacac gacgaccctg gccgaacccg aggtcgtcga gcggctgatc
     3241 gccgagcaca aggcgcgggg cggctgaacc ggccggccgc ggctcagaac aggccgaaca
     3301 ggttgagcag caccacgaag atcacgaccg ggcagacgta gcggatgaac aacccccacg
     3361 ccgcgatcgt gaccggcgga acccggctgc cctcgcgcag ttcggcaccg gccggcgcca
     3421 cgccccacac ccaaccgacg aagacgctga gcagcagcgc gccgatcgcc agcgacaggt
     3481 tgccccagat gaagtccatc accccgagga acgaggcgtt gccgtggagc gagatcgtgc
     3541 tgagcgtcga cgacgcgccc tgggacagcg ccgacggcag accgacgagg aaggtgaaga
     3601 tgccgaccgc gatcaccgcg cgccggcgcg accagcgtcg ctcgtcgatg aagtacgcca
     3661 ccacgacctc gagcagcgag accgtcgacg tcaacgcggc gatcgacagc agcacgaaaa
     3721 agacgatcgc caccgggata ccgaacggca tcgccgcgaa gatctccggc agcacttcga
     3781 acaacaacgt cgggccggcg gcggggttct tgcccatggc gaataccgcc gggaagatca
     3841 tgaagccggc gagcaacgcg accaacgtgt cggacaccgc gacccacaac cccgacatct
     3901 gcaggtcggc ggtccgcggc agatacgagc cgtaggtcac catcgcgccc atgccgaggc
     3961 tcaacgagaa gaaggcttga ccgagtgccg tcagcagtgt tcgtccggtg acctgcgaga
     4021 agtcgggttt caagtagaac tccagaccgg cgccggcgcc cggcaaggtc aagccgcgtg
     4081 cgatgaccaa cagcatcaac accaacagca ccggcatcag gatcttcgac caacgctcga
     4141 tgccgtgctc gatcccgccg agcacgacca acatcgtcag cgcgatgaac caggcgaaca
     4201 acggaatgac gatcgccggc gacgcggtga attcggcgtg cggcatgtcg ggggcgatca
     4261 gcgtcttgag gatgtagccg aaggcccacc cggcgatgac ggcgtagtag ctgaggatga
     4321 agacgccggt cagcacgccg aggccgccga cgacgggcca cgccgagcgc ggcttgagtg
     4381 cgaggatcgc gccgaccggg ttcttcgcgg tccggcggcc gacggccagc tcggcgaaca
     4441 ggtacggcag gcagatcaac agcacgcacg ccaggtacaa cagcacgaac gccgcgccgc
     4501 cgtgcgtgcc gacgacgtag ggaaagcgcc agatgttccc caatccgatc gccgagcccg
     4561 aggcggcgag cacgaagccc accgacgagc cccactggcc gcgttcctgc tgcatcgccc
     4621 ccctcctcga ccgccgcgtg gtcgactcgc cggtcgcgcg gccgggcagt gtaagaggtc
     4681 gatcccgcgc cggtcaactg cgctcgccgg caaagaactg gcccggcttc gattcgtata
     4741 agatcagacg aggaccgcgc agcaaccccg cggcggccca gggagccgaa acgatggaca
     4801 agatgaagct gctttcgtcc ggtttgctgt tgttgctcgc cggcctgccg accttcgccc
     4861 agcaggggca gggcgtcgac gcggcgggac gctcgcccgc ggcgatcgag atcctgcaga
     4921 aggtcgacgc ggcggtcaag gccatctcgg ccgtgcggta cgacgtcaag accgttcccg
     4981 gcggcgtcgc cgagcagatg gtcggtccgg gcgaagcctc ggtcgtgctg atcggttgga
     5041 acgggcggat cccggagaag ttccacgtcg agggcaagac cgcgcgtggc gggcaaccgg
     5101 tcaccgtcac cggcgggggc aacggcgaca gttacttcct cgtcgaccac acgaccaaga
     5161 aaggctacga ggacatggat ccggcggtgt tgggcggtag cgcgcaggcg ttgatgggct
     5221 ttgccatgct cgagttcgtc cacgatcggc cgttcgacga cgagctcgcc gccgaggtcg
     5281 ccgagctgcg gggcagcaag acgatcggcg gggtcgactg ccacgagatc cgcgtggtct
     5341 actccagcgg gcagggcgag tcgatctggt acttctcgaa gaccgactac accccccgcg
     5401 gccgcgtccg cgtgttcaac acgccgcagg gcgaaggcac gatcaccgtc acgatcgaga
     5461 acctcgagat cgatccgaaa gtcgacgcga cgctgttcgt gatgaacctc ccggcggggt
     5521 acgaagagat cgacgacttc gctccgtaag gcggcgcgtc aacccagatc gagcgccatg
     5581 atccggctct tgtcgcggac gtacatcgtc ttgccggcga tcgtcggtac ggtccaggcg
     5641 acgtcttcgg tcaagtcgat cttcgagtgc agcaccaggt cgtccgggct ggcggtggtc
     5701 aggtacagca cgccgtcctc gtcgaggatg aacagctggc cgtcggcgga gacgcagttg
     5761 gccttggcgt aaccgcgctt gcgccaggcg atctcgccgg tcttgagatt caacgccgac
     5821 atgaacgacg gcgccatcgt accggtcgag ccgtagacgt agtccccgtc gcggacggtg
     5881 ttgacgtggt agaactggat cttgcgcgtc gaccaggtct cctcgaccac gaagccgtcg
     5941 ccgttgtcgg tcagcttgag cgccttggcg ccggcttgcg gcgacgagat gaacaggtgg
     6001 tcgtcgtcga tcttgatcgg catggtgatg ttctgttccc attggttctg gtgcggatgg
     6061 ctccacagca ccgcgccgtc ggccgggtcc atgccgacga ccgcgtcggc catgaacgtg
     6121 atcaactgct cccgacccgc caattccagc acgcgcggcg tcgagtagct gttcttgaag
     6181 tcaccggcct tccacttcgt ctcgcccgtc ttcttgtcga aggcgacgac cgagcgtccc
     6241 tcgccgccga cgagcgcgat caccgtgtcg ccgtactcga tcgggctcga cgagtagcca
     6301 tggttca
//
LOCUS       DASPGN010000219         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_154160, whole genome shotgun sequence.
ACCESSION   DASPGN010000219 DASPGN010000000
VERSION     DASPGN010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154160"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2073
                     /locus_tag="VD788_06270"
     CDS             <1..>2073
                     /locus_tag="VD788_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75910.1"
                     /translation="DVYLQNAATVGSVTGCTTIESVFYAGTSPEVSWTGNTFDNWGTR
                     TSRAAIAVLGSLTSANTFNPVPGAVLQVTGGTVAEDAAWAAAAGPLVFDAASITVAGT
                     DGADARTTLSLGPGTTLRLPANAVFNVGTSAGSPGELIADGRLSAGLVASVTFDSSLA
                     APSTGAWSGIRVRPNGRAELFETQVRNADVALWIEGTLGPAEVLGVNRANVGLLLDGA
                     TVEQTLRLLDFRNVSVAVSADATAVTVRDSNLVGSTYGAQNFTPATHCVDAALNWWGA
                     ASGPSGNATPEGCEVDAPSGSGSPLSAGVIFDDPLTVPPDDGDPIACDDGDGLADPCT
                     GGLTVDCDDNCCLVANASQSDLDNDGTGDACDVNPTLRVSNDPADGADFSVIQDAVDA
                     SFQSGSRIEILSGLAPYAESVRLDRQQVYFIRGVENGNGDPVIVDGLGGAAFHVVNKV
                     GAVPTSFRTLTLRGDQGIHAAVDTDVTDIVFDTIAHEALEIDAGQHRVRLCTIDGPAV
                     GARISSGASLDLERTMVVGAIDAGLVVEGAVAARNVILGDGLDGVRPGPGGTVTLSYV
                     TIAGNTGYGIDNTLAGAVGVDRSILWGNSLGDLLGVACGAVTWSLVGSPDCSASGDNI
                     IGDPLLDGDFLPATSSPCVDHGPDPASYDGEPPADFDGGARLLDADGDGLAQNDCGAF
                     EVEAVPGGP"
BASE COUNT          296 a          733 c          755 g          289 t
ORIGIN      
        1 cgacgtctac ctgcagaacg cggcgaccgt gggcagcgtg acgggctgca cgacgatcga
       61 gagcgtgttc tatgcgggca cgtcgccgga ggtgagctgg accgggaaca cgttcgacaa
      121 ctggggcacg cggacctcgc gggcggcgat cgccgtcctc ggctcgctga cgtccgccaa
      181 cacgttcaac cccgttccgg gcgcggtgct gcaggtaacc ggtggcacgg tcgccgaaga
      241 cgctgcgtgg gcggccgccg cagggccgct cgtcttcgac gcggcgtcga tcacggtcgc
      301 ggggaccgac ggcgccgacg cgcgcacgac gttgagcctc ggcccgggga ccacgctgcg
      361 cctgccggcg aatgcggtgt tcaacgtcgg gacgagcgcg gggtccccgg gcgagctgat
      421 cgccgacgga cgactgtccg cagggctcgt cgcctccgtg acgttcgaca gctcgctcgc
      481 ggctccgtcc accggcgcgt ggtccggcat ccgcgtccgg ccgaacggcc gcgcggagct
      541 cttcgagacg caggtgcgca atgccgacgt cgcgctgtgg atcgagggca cgctcggccc
      601 ggccgaggtc ctcggcgtca accgcgcgaa cgtgggtctc ttgctcgacg gcgcgacggt
      661 cgagcagacg ctgcgcctgc tcgacttccg caacgtatcg gtggccgtga gcgccgacgc
      721 gacggccgtg acggtgcgcg acagcaacct cgtcggctcg acgtacggag cgcagaattt
      781 cacgccggcg acgcactgcg tcgacgcggc gctcaactgg tggggggcgg ccagcggccc
      841 gtccggcaac gcgacgccgg aggggtgcga ggtcgacgcg ccgagcggca gcggctcacc
      901 gctctccgcc ggggtgatct tcgacgatcc gctcaccgta ccgccggacg acggggaccc
      961 gatcgcctgc gacgacggcg acggactcgc cgatccgtgc actggcggac tgacggtcga
     1021 ctgcgacgac aactgctgtc tcgtggccaa cgcctcgcag tccgacctgg acaacgacgg
     1081 aacgggcgac gcgtgcgacg tgaatcccac gctgcgcgtc tcgaacgacc cggccgacgg
     1141 cgccgacttc tccgtgatcc aggacgccgt ggacgcgtcg ttccagtccg gcagccgcat
     1201 cgagatcctc tccgggctcg cgccctacgc ggagtcggtc cggctcgacc gccagcaggt
     1261 ctacttcatc cggggcgtcg agaacggaaa cggcgacccc gtgatcgtgg acggtctcgg
     1321 cggtgcggcg ttccacgtcg tcaacaaggt cggcgcggtg cccacgagct tccgcacgct
     1381 gacgctgcgc ggcgaccagg ggatccacgc ggcggtcgat accgacgtca ccgatatcgt
     1441 gttcgacacg atcgcgcacg aggcgctgga gatcgatgcg ggtcagcatc gggtccggct
     1501 gtgcacgatc gacggcccgg cggtgggtgc gcggatctcc tccggcgcga gcctcgatct
     1561 cgagaggacg atggtcgtcg gggcgatcga tgccggcctc gtcgtcgaag gcgccgtggc
     1621 cgcgcggaac gtgatcctcg gcgacggcct cgacggagtg cgtccgggac ctggcggcac
     1681 cgtgacgctg tcctacgtca cgatcgccgg caacaccggg tacggcatcg acaacaccct
     1741 ggccggcgcg gtcggcgtcg accgctcgat cctgtggggc aactccctgg gggacctcct
     1801 cggcgtcgcg tgcggcgcgg tgacgtggag cctcgtcggg agcccggact gcagcgcctc
     1861 cggcgacaac atcatcgggg atccgctgct cgacggcgac ttcctgcccg cgacaagctc
     1921 gccctgtgtc gaccacggcc ccgacccggc ttcctacgac ggcgagcctc cggccgactt
     1981 cgacggcggg gcacggctgc tcgacgcgga cggcgacggt ctggcgcaga acgactgcgg
     2041 ggcgttcgag gtcgaagcgg tccccggcgg ccc
//
LOCUS       DASPGN010000220         2376 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_505886, whole genome shotgun sequence.
ACCESSION   DASPGN010000220 DASPGN010000000
VERSION     DASPGN010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505886"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VD788_06275"
     CDS             complement(<1..1064)
                     /locus_tag="VD788_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HXV75911.1"
                     /translation="MNRPRAVRAWAVAWLVVLSACRSSPTPDAPEPWFEETARRDGLD
                     FVHQSGHGGELLFPEIMGGGVALFDMDGDGDLDAYLVQSGRLLESPADEAGNRLFENV
                     GAGRFVDVSAGSGAGDTGYGMGAAAGDYDGDGDTDLYVTNFGPNVLLRNEGGGRFRDT
                     TGDAGVGDPSWSASAAFLDYDVDGDLDLFVANYVNWTPSIEQRCYNAAGVRDYCLPTN
                     YHAPAMDRLYRNRGDGTFEDVSIDVGLQTAFGNGLGVVSSDFDGDMRPDIFVANDMMM
                     NQLWMNRGGRFVDEALLRGNAVDEHGSPKAGMGVMAIDINDDGNEDLLVGNLQGQTDS
                     FFINRGGTFSDATGSVGLGSA"
     gene            complement(1061..>2376)
                     /locus_tag="VD788_06280"
     CDS             complement(1061..>2376)
                     /locus_tag="VD788_06280"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75912.1"
                     /translation="LLIDLGEAHPESAAKLTALYLVARDQPLDARARGQLGMAYEING
                     FRDAAKTTYAQAADLAPDDPRWTYLAALLLAADGEVEQALDAMDRSLAIDDLYVPGHL
                     YRSQWLLDTGDAQAAEQGYREVLRLDPDNRSARLGVARALIRQQRSAEAVELLEAYGE
                     GRPNDAYAQQLLGTAHRELGNLEAANAALARGRTDAAPSRWPDPRAEEKLRFVSGYGA
                     DMLHGERLLEAGRTDEAITVFEALFTERSEDLQLINNLAVAYRRAGQTERALDLLLES
                     VESHPDYFPFFMNLGAVYQDAGQTEQALGAFERASELNPTLAQAHRRRARLLTELGRR
                     QEALDAFETAISYEPTDRIAITYAGMLATELGQYERAVGWLEKSVELEPRMLPTLLAL
                     GECKAYLGDFEGADEALERAAALQPDSKSLVEIRSRVDELRTRGS"
BASE COUNT          355 a          827 c          806 g          388 t
ORIGIN      
        1 gcgctaccga gaccgaccga gccggtcgcg tcgctgaacg tcccaccgcg gttgatgaag
       61 aacgaatcgg tctgtccctg caggttgccc accagcaggt cctcgttgcc gtcgtcgttg
      121 atgtcgatcg ccatcacgcc catgccggcc ttgggcgacc cgtgttcgtc caccgcgttg
      181 cctcgcagca gcgcctcgtc gacgaagcga cctccccggt tcatccagag ctggttcatc
      241 atcatgtcgt tggcgacgaa gatgtccggc ctcatgtcgc cgtcgaaatc ggaactgacg
      301 acgccgaggc cgttgccgaa cgccgtctgc aatccgacgt cgatcgatac gtcctcgaac
      361 gtcccgtcgc cgcggttgcg atacaggcgg tccatcgccg gcgcgtggta attggtcggc
      421 agacagtagt cgcggacgcc ggccgcgttg taacatcgct gctcgatcga gggtgtccag
      481 ttgacgtagt tggcgacgaa caggtcgagg tcgccgtcga cgtcgtagtc gaggaacgcg
      541 gcgctcgcgc tccacgacgg gtcgccgact cccgcgtcgc cggtcgtgtc gcggaaccgc
      601 ccgcccccct cgttgcgcag caggacgttc ggaccgaaat tggtgacgta caggtcggtg
      661 tcgccgtcgc catcgtagtc gccggcggcc gcacccatgc cgtagccggt gtctcccgct
      721 ccgctgccgg cgctgacgtc gacgaatcgg cccgcgccga cgttctcgaa caggcggttg
      781 cccgcctcgt cggccggcga ctcgagcagc cggccgctct ggaccaggta ggcgtccagg
      841 tcgccgtccc catccatgtc gaacaacgcg acgcccccac ccatgatctc ggggaacaag
      901 agctcgccgc cgtggcccga ctggtggacg aagtccaggc cgtctcgccg agcggtctcc
      961 tcgaaccacg gctccggcgc gtccggggtc ggagaagaac ggcaagcgga caggacgacg
     1021 agccaggcga ccgcccacgc acggaccgcg cggggccggt tcatgagcct cgggttctca
     1081 actcgtcgac ccgactccgg atctcgacca agctcttgct gtccggctgc agcgcggccg
     1141 cgcgctcgag cgcttcgtcc gcaccctcga agtcgcccag gtaggccttg cactcgccca
     1201 gagcgagcag ggtcggcagc attcgcggct cgagctcgac cgacttttcg agccacccca
     1261 cggcgcgctc gtactgcccc agctccgtcg cgagcattcc ggcataggtg atggcaattc
     1321 ggtccgtcgg ctcgtagctg atcgcggtct cgaacgcgtc gagcgcctct tgccggcgac
     1381 ctagttcggt caacaggcgg gcccgccggc gatgggcctg agccagcgtc gggttgagct
     1441 cgctggcgcg ctcgaaggcg ccgagggcct gctcggtctg ccccgcgtcc tggtagaccg
     1501 cgccgaggtt catgaagaac gggaagtagt ccgggtggct ctcgaccgat tccagcagca
     1561 ggtcgagggc ccgttcggtt tgacccgcgc gacgataggc gaccgcgagg ttgttgatca
     1621 actgcaggtc ctcggagcgc tcggtgaaga gtgcctcgaa caccgtgatc gcttcgtcgg
     1681 ttcggccggc ttcgagcaag cgctcgccgt gcagcatgtc ggcaccgtaa ccggagacga
     1741 accgcagctt ctcctcggcc cgggggtcgg gccagcgaga cggcgcagcg tcggtccggc
     1801 cgcgagccag ggcggcgttc gccgcctcga gattgcccag ctcgcgatgg gcggtcccga
     1861 ggagctgttg tgcgtaggca tcgttcggac gcccctcgcc gtaggcctcg agcagctcga
     1921 ccgcctcggc cgagcgctgt tggcggatca gtgcccgggc cactccgagc cgggccgagc
     1981 ggttgtcggg atcgagacgc agcacctcgc ggtacccctg ctcggccgcc tgggcatcgc
     2041 ccgtatcgag cagccactgg gatcggtaga gatggcccgg gacgtacagg tcatcgatcg
     2101 cgagcgaccg atccatcgcg tcgagcgcct gctcgacctc gccgtccgcc gccaggagca
     2161 gcgccgcgag gtacgtccag cgcggatcgt cgggcgcgag gtcggcggcc tgggcatagg
     2221 tcgtcttggc ggcgtcgcga aagccgttga tctcgtaggc catgcccaat tgaccgcggg
     2281 cgcgagcgtc gagcggctga tcccgcgcca ccaggtagag cgcggtcagc ttcgccgcgg
     2341 attccgggtg agcctcgccg agatcgatca gcagcg
//
LOCUS       DASPGN010000221         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_272879, whole genome shotgun sequence.
ACCESSION   DASPGN010000221 DASPGN010000000
VERSION     DASPGN010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272879"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VD788_06285"
     CDS             complement(<1..249)
                     /locus_tag="VD788_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75913.1"
                     /translation="MSELRRFSCVLSGLLLVAGSAFAGTDELRVSVTATGAPGGAVVA
                     TDSDGAPDDGVATKGVIAVDFLVPGTAFAAGQAIGSLTV"
     gene            424..1125
                     /locus_tag="VD788_06290"
     CDS             424..1125
                     /locus_tag="VD788_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXV75914.1"
                     /translation="MRILVVEDYPAIARSLERQLRAILGVQVDRVTIATDLRAASTAL
                     RDDAVDLILLDLNLGGGDGFDLLRWAAAVAAHTIVVSGNTQRALEAFEHGVLDFVPKP
                     VEPARLRRALARLNPRSAGADRPATRRLAVADGPGYRLIELATVRRIQAVGKYSEIHP
                     VEGRPRIHDKPLERLEALLPDGFVRVHRSHVVNLTFARRLDVRSGGRYRLVLDDGSAV
                     PVGRTRYAALRERMF"
     gene            complement(1122..2735)
                     /locus_tag="VD788_06295"
     CDS             complement(1122..2735)
                     /locus_tag="VD788_06295"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXV75915.1"
                     /translation="MRDADLVVARTPYLHRAGDDPGWAAAEPADPTAWTTTRPRERGP
                     WWFRTTLMLEREARVEDDLALRVLAPGAIEVFWDGERVGGSGRIGHDAVSERPGRHGL
                     WVPLAPARVTAGEHVVAVRVSSWRLSFVHGPREVLLLDRGATERVETLRTGAFLFCAG
                     LLVLAGVVFELLRRPTGARRDTELFATLTLLVALLLLLEYAKTLYPYPYTWHFPRLVA
                     ISTLTMCVGILLPVFASAHLTIWRPWFVAATMCALFVGLVVAFPDPDVHSLNMLRASI
                     AGTALLALVGVVRGSDGATWILLAAAANVPPIVVSGMRYADHWFFVGFLAMVGVLLLR
                     MAGTLRRRAGERDLALLRAERLRNELLRKTIQPHFLMNSLAVAIAQIEEEPARGVALL
                     HDLASELEAFLAIGDRDLVPVREEIELCRKHVRTMAHLLDRPLLLEVEGRLGSLELPP
                     GVLLTLVENAITHGNHGGDARITLRIADEADGLTIDVANASPPSAATAEGAGLRYVRS
                     QLEHRPGEDWAIRCGHESGRWHTRLHRSR"
     gene            2941..>3486
                     /locus_tag="VD788_06300"
     CDS             2941..>3486
                     /locus_tag="VD788_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXV75916.1"
                     /translation="MRVRHARFGSSIVVTLVAVASAASAAPGADPGSGPWLQYSMVEL
                     AGFDPAKLEQVRRQAEAAGSGALLVVRNGSVVVAWGDVERKFKCHSVRKSFLSALYGQ
                     AVARGEIDLAATLGQLGIDDREPLSAIEKQATVEQLIQARSGVYHPAAKEPGAMKEDR
                     PVRGSRAPGEAFWYNNWDFNTA"
BASE COUNT          533 a         1226 c         1256 g          471 t
ORIGIN      
        1 gacggtcagc gagccgatcg cctggcccgc ggcgaaggcg gtccccggga ccaggaaatc
       61 gacggcgatc acgcccttcg tcgcgacccc gtcatcgggc gcgccgtcgc tgtcggtcgc
      121 gacgaccgca ccacccggcg cacccgtcgc cgtgacgctc acccgcaact cgtccgtgcc
      181 ggcgaacgcg ctgcccgcca ccagcaagag tcccgacaga acgcaggaga accgccgaag
      241 ctcgctcatg atcgatctcc atacgacgtt tcacaacaat cgcaaagtca gatactggtt
      301 agatacatct gcatatgtgt gtttctcaat agttaatttg gtatggatgg actggtgatc
      361 ggcccggcga agccgaattc cccgacgcga tccggcgccg ggccggggcg ctacgatgaa
      421 cgggtgcgga ttctcgtcgt cgaagactac cccgcgatcg cgcggtcgct cgaacggcag
      481 ctgcgcgcga tcctcggcgt gcaggtcgac cgggtgacga tcgccacgga cctccgcgcg
      541 gcgagcacgg cactgcgaga cgacgccgtc gacttgatcc tgctcgacct gaacctcggc
      601 ggaggcgacg gcttcgatct gctgcgttgg gcggccgcgg tggccgcgca cacgatcgtg
      661 gtctcgggca acacgcagcg ggcgctcgag gcgttcgagc acggcgtgct cgacttcgtg
      721 ccgaagccgg tcgagccggc gcggctgcgg cgcgccctcg cgcgcctgaa tccgcgctcg
      781 gctggagcgg accgccccgc gacacgccgg ctggcggtcg cggacgggcc gggttaccga
      841 ttgatcgaac tcgccacggt gcgccggatc caggcggtcg gcaagtactc cgagattcac
      901 ccggtcgagg gacgcccgcg catccacgac aagccgctcg agcggctcga ggcgttgcta
      961 cccgacggct tcgtccgcgt gcaccgctcg cacgtcgtca acctgacgtt cgcgcgccgg
     1021 ctcgacgtcc gctccggcgg ccgctacagg ctggtactcg acgacggcag cgcggtcccg
     1081 gtcggtcgca cgcggtacgc ggcgcttcgc gagcggatgt tctagcggga gcgatggagg
     1141 cgggtgtgcc agcggccgct ctcgtggccg cagcggatcg cccagtcttc gcccgggcgg
     1201 tgctcgagtt gcgagcggac gtagcgcagg ccagcgcctt cggcggtcgc cgcggacggg
     1261 gggctggcgt tggcgacgtc gatcgtcagg ccgtcggctt cgtcggcgat gcggagcgtg
     1321 atccgggcgt cgcctccatg gttgccgtgg gtgatggcgt tctcgacgag agtcagcagc
     1381 accccggggg ggagctcgag agacccgagc cgaccttcca cctcgagcag cagcgggcgg
     1441 tcgagcaggt gcgccatcgt gcgcacgtgc ttgcggcaca gctcgatctc ctcccgcacg
     1501 ggtacgagat cgcggtcgcc gatcgcgagg aaggcctcga gctccgacgc cagatcgtgg
     1561 agcagagcga caccgcgcgc gggctcctcc tcgatttgcg cgatcgcgac cgcgagcgag
     1621 ttcatcagga aatggggctg gatcgtcttg cgcagcagct cgttgcgcag ccgttccgcg
     1681 cgcaacagcg ccaggtcgcg ctcgccggcc cggcggcgca gggtgccggc catccgcagc
     1741 agcagcacgc cgaccatcgc caggaagccg acgaagaacc agtggtcggc gtaccgcatc
     1801 ccgctgacga cgatcggcgg cacgttcgcc gcggcggcga gcaggatcca cgtggccccg
     1861 tccgagccgc ggacgacgcc gacgagggcc aacagcgccg tcccggcgat cgaggcgcgc
     1921 agcatgttca ggctgtgcac gtccggatcg ggaaacgcga cgacgaggcc gacgaacaac
     1981 gcgcacatcg tcgcggcgac gaaccacggc cgccagatcg tcaggtgggc cgacgcgaag
     2041 accggcaaca ggattccgac gcacatggtc aaggtcgaga tcgcgacgag gcggggaaag
     2101 tgccaggtgt aggggtacgg gtagagtgtc ttcgcgtact cgagcagcag cagcagcgcg
     2161 accagcaggg tcagcgtggc gaacagctcg gtgtcgcgcc gcgctccggt cgggcggcgc
     2221 aaaagctcga agacgacgcc ggccaggacg agcaggccgg cgcagaagag gaaggccccc
     2281 gtgcgcagcg tctcgacgcg ctccgtcgcc ccacggtcga gcagcagcac ttcgcgcggt
     2341 ccgtggacga aggacaggcg ccagctcgac acgcgtacgg cgacgacgtg ctcgccggcg
     2401 gtgacgcgcg cgggcgcgag cggcacccag aggccgtgcc ggcccggacg ctcggagacc
     2461 gcgtcgtgcc cgatgcgtcc gctcccgccg acccgttcgc cgtcccagaa cacctcgatc
     2521 gcgcccgggg cgagcacccg gagcgcgagg tcgtcctcga cgcgcgcctc gcgctcgagc
     2581 atgagcgtcg tgcggaacca ccacgggccc cgctcccgcg gccgcgtcgt cgtccacgcg
     2641 gtcgggtcgg ccggttcggc cgcggcccag cccgggtcgt caccggcgcg gtggaggtac
     2701 ggcgtgcgcg ctacgacgag gtccgcgtca cgcacgatct cctccggcgc gcagccgacg
     2761 gccgtggcca gcaggccgac gacggcgagg ccggcgatcc ggcggtgccg ttcgttccgt
     2821 cgacgtgccg ttcgtcccgt ttgcggccgg cggcccggaa cctgccgcgc ggcgctcacg
     2881 tctctacggt cacgcacgcg gagacggcac caggtacccc gctgcgggag gaggctcgac
     2941 atgcgcgttc gccacgctcg cttcggatcg tcgatcgtcg tcaccctcgt cgcggtggca
     3001 tcggcggcct cggccgctcc gggcgccgat cccggctccg gtccgtggct gcagtactcg
     3061 atggtcgagc tcgccggctt cgatcccgcg aagctcgagc aggttcgccg gcaggccgag
     3121 gcggccggct cgggtgcgct gttggtcgtc cgcaacggca gcgtcgtcgt cgcgtggggc
     3181 gacgtcgagc ggaagttcaa gtgccactcg gtgcgcaaga gcttcctctc cgcgctgtac
     3241 ggtcaggcgg tcgcgcgcgg cgagatcgac ctcgcggcga cgctcggcca gctcggcatc
     3301 gacgaccgcg agccgttgag cgccatcgag aaacaggcga ccgtcgagca gctgatccaa
     3361 gcgcgatcgg gagtctacca cccggcggcg aaggagccgg gcgcgatgaa ggaggaccga
     3421 ccggtgcgcg gcagccgcgc ccccggcgag gcgttctggt acaacaactg ggacttcaac
     3481 accgcc
//
LOCUS       DASPGN010000222         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1091872, whole genome shotgun sequence.
ACCESSION   DASPGN010000222 DASPGN010000000
VERSION     DASPGN010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1091872"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VD788_06305"
     CDS             complement(<1..897)
                     /locus_tag="VD788_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HXV75917.1"
                     /translation="MARPTAIVLSMVLLDAIAGATAAETDRAIPSRLLTVAERTDFRA
                     TASYEETLDFIRGLSREMPEIGLETFGVSPEGRPLPLVVVAAAGADDPARAAAAGKAV
                     VLIQNGIHAGEIDGKDACLMILRDMVRGRHRAWLDSLVLLIVPIYNVDGHERVSPYNR
                     ANQNGPVDGMGFRTTADGHDLNRDHLKLETPEARAMIGLFNRWRPQLHVDNHVTNGSD
                     HDWVLTYSWAEAPQLAPSLHDWVERILPVVVERTANHGHRLGPYVWLLDDTDPSAGFR
                     SWVGEPRYATGYYPLRNRPSILV"
     gene            complement(955..2184)
                     /gene="dinB"
                     /locus_tag="VD788_06310"
     CDS             complement(955..2184)
                     /gene="dinB"
                     /locus_tag="VD788_06310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXV75918.1"
                     /translation="MSRGGPQRVILHLDMDAFYAAIEIRENPALAGQPLIIGYRGRRG
                     VVSTCSYEARRYGVRSAMPSLRAERLCPNAIWLNGRMALYVEVSRAIRSLMHEVSPVV
                     EPLSIDEAFIDLSGIAADLAGGVEIARSLKRRIAETQRLTASAGVASNKFLAKLASDI
                     DKPDALTRIAIDDLERVVWPLAVDRLWGVGPKTAARLRAAGIETIGDLLRSEPARLES
                     VVSEAMAEQLRALARGEDDRRVVSDRSHKTISEERTYGRDLLDADAIDRALLARSEGV
                     ARQLRRERMIAHTVRLKVRAGDFTTWTRSRTLPAPTDLTEEIVRAARELFRERVALGG
                     RGVRLLGVGVGNLEPAERYQSGLFDDPAAERARRMARVTDAVCARLGERAMTRARLLP
                     RRAPTDDGEASSLPTVD"
     gene            complement(2181..3122)
                     /locus_tag="VD788_06315"
     CDS             complement(2181..3122)
                     /locus_tag="VD788_06315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXV75919.1"
                     /translation="MRAMAIDAFGGPERLRPGDRPRPKPRAHELLIRTVAAGVNPVDA
                     RIVGGRLPSGSSHAFPLIPGFDVAGVVDELGERTSRLRKGDRVWALALAASLQWGSYA
                     EFVAVDESSVALMPTKLLYEEAAAVPLAALAARQALFDEGRLATGGSVLIHSGSGGVG
                     HFAVQLAKSAGCRVLATGGPANQGFLAEIGADVPIDHTRVDLRDAVGREVPGGVDLVL
                     QTVDPANLTRSLDLVRPGGRLVSTVGLPGGVPRTGAAIRQLSVKPSADGLRSLAALVD
                     RGALRPHVSAILSLEQAPEALRRIAAGHVRGKLVLNL"
     gene            3212..>3416
                     /locus_tag="VD788_06320"
     CDS             3212..>3416
                     /locus_tag="VD788_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXV75920.1"
                     /translation="MATCVELSVEAGLATVRLSRPHGNAINGQLVDDLTRVCGELAAD
                     PTIRGMMLAARGRIFCPGLDLHEL"
BASE COUNT          500 a         1236 c         1206 g          474 t
ORIGIN      
        1 gaccagaatc gacggccggt tgcgcagcgg gtagtagccg gtggcgtaac gcggttcgcc
       61 gacccaggag cggaaaccgg ccgacgggtc ggtgtcgtcg agcagccaga cgtagggtcc
      121 cagccggtgt ccgtggttgg cggtccgctc gaccacgacc ggcaggatcc gctcgaccca
      181 gtcgtggagc gacggcgcga gctgcggcgc ctcggcccag ctgtaggtca gcacccagtc
      241 gtggtccgag ccgttcgtca cgtggttgtc gacgtggagc tgcggccgcc agcggttgaa
      301 cagaccgatc atcgcacgcg cttcgggggt ctcgagcttc aggtggtcgc ggttcagatc
      361 gtgcccgtcg gcggtcgtgc ggaagcccat cccgtcgacc ggtccgttct ggttggcgcg
      421 gttgtagggc gagacgcgct cgtgcccgtc gacgttgtag atcgggacga tcaacagcac
      481 gagcgaatcg agccacgcgc ggtgccggcc gcgcaccatg tcgcgcagga tcatcagaca
      541 cgcgtccttg ccgtcgatct caccggcgtg aatgccgttt tggatcaaca cgaccgcctt
      601 gcccgccgcc gcggcccgcg cggggtcgtc cgcaccggcc gcggcgacga cgaccagcgg
      661 cagcggtctg ccctcgggcg agaccccgaa ggtctcgagg ccgatctccg gcatctcgcg
      721 cgacaacccg cggatgaagt cgagcgtctc ctcgtaggac gcggtggcgc ggaagtccgt
      781 gcgctcggcg accgtcagca gccgcgacgg gatcgcccgg tcggtttccg ccgcggttgc
      841 accggcgatc gcgtcgagca gcaccatcga aagcacgatt gccgtcggtc gggccacggt
      901 cggtctccag cggtgaggcg agcactctag caaacccgcc gcgggcgctg cgccctaatc
      961 gacagtcggc agactcgacg cttcgccgtc gtcggtcggc gcgcgccgcg gaagcagccg
     1021 tgcgcgcgtc atcgcccgct cacccagccg ggcgcagacg gcgtcggtca cacgggccat
     1081 gcgccgggcg cgctccgcgg ccgggtcgtc gaacagtccg ctctggtagc gctcagcggg
     1141 ctccaggttg ccgacaccga caccgagcag ccgcacgccg cggcctccga gcgcgacacg
     1201 ctcgcggaac agttcgcgcg ccgcccgcac gatctcctcg gtgagatccg tcggtgcggg
     1261 cagcgtccgc gaacgggtcc aggtcgtgaa gtcgccggcg cgtaccttga gccggaccgt
     1321 gtgcgcgatc atccgctcgc gccgcagctg gcgcgcgaca ccctcgcttc gggcgagcag
     1381 cgcgcggtcg atcgcatcgg cgtcgagcag gtcccggccg taggtccgct cctcggagat
     1441 cgtcttgtgc gaccggtcgc tgacgacgcg gcgatcgtcc tcgccgcgcg cgagcgcgcg
     1501 caactgctcg gccatcgcct cgctgacgac cgattcgagc cgcgccggtt cgctgcgcag
     1561 cagatcgccg atcgtctcga tgcccgcggc gcgcaatcgt gcggcggtct tcgggccgac
     1621 gccccacaga cggtcgacgg ccagcggcca caccacgcgc tcgaggtcgt cgatcgcgat
     1681 cctcgtcagc gcgtcgggct tgtcgatatc ggaagccagc ttggccagga acttgttcga
     1741 cgcgaccccg gccgacgcgg tcagccgttg ggtctcggcg atccggcgct tcagcgatcg
     1801 agcgatctcg acgccaccgg cgagatcggc ggcgatcccg ctgagatcga tgaacgcctc
     1861 gtcgatcgac agcggctcga cgaccggcga gacctcgtgc atcagagagc ggatcgcgcg
     1921 cgacacctcg acgtacagcg ccatgcgccc gttgagccag atcgcgttcg gacacagtcg
     1981 ctccgcccgc aacgagggca tcgccgagcg cacgccgtag cggcgcgcct cgtaggagca
     2041 ggtcgagacg actccccgtc gaccccgata cccgatgatc aacggttgac cggccagcgc
     2101 cggattctcg cgaatctcga tcgccgcgta gaacgcgtcc atgtcgaggt gcaggatcac
     2161 gcgctggggc ccgccgcggc tcacaggttg agcaccagct tgccgcgaac gtggccggcc
     2221 gcgatccggc gcaacgcctc gggcgcttgc tcgagcgaga ggatcgccga gacgtgcggg
     2281 cgcagcgcac cgcggtcgac gagcgcggcg agcgagcgca gcccgtcggc gctcggcttg
     2341 accgacagct ggcgaatcgc cgcgccggtc cggggcaccc cgccggggag cccgaccgtg
     2401 ctgaccaacc gcccgccggg ccgcaccagg tcgagcgagc gggtcaggtt ggccgggtcg
     2461 accgtttgca gcacgaggtc gacgccgccg gggacctccc gcccgacggc gtcgcgcaga
     2521 tcgacccgcg tgtggtcgat cggcacgtcc gcaccgatct cggccaggaa cccctgattc
     2581 gccgggcccc cggtggccag cacgcggcag cccgcgctct tggccaattg gacggcgaag
     2641 tggccgacgc cgcccgagcc cgagtggatc agcaccgagc ctccggtggc caagcggcct
     2701 tcgtcgaaca gcgcctgccg ggcggccagt gccgcgagcg ggacggccgc cgcctcttcg
     2761 tagagcagtt tcgtcggcat caacgccacc gacgattcgt cgaccgcgac gaactccgcg
     2821 taggagcccc actgcaacga cgccgcgagc gccagcgccc agacccgatc gcccttgcgc
     2881 aagcgcgagg tccgctcgcc cagctcgtcg acgaccccgg cgacgtcgaa ccccggaatc
     2941 agcgggaagg cgtggctcga cccgctgggc agccgtccgc cgacgatcct cgcgtcgacc
     3001 gggttgaccc ccgcggccac ggtgcggatc agcagctcgt gcgcccgggg cttcggtcgc
     3061 ggccggtcgc cgggacgcag tcgctccggc ccgccgaacg cgtcgatggc catcgctcgc
     3121 atcgctccac tcctcagcgc cgcgtcgttc cggcgatcga caccatgata tcgcgggcga
     3181 cgggatacaa tggccggacg gaggtggagc ggtggcgacg tgcgtcgagc tgtcggtcga
     3241 ggcggggctg gcgaccgtgc gattgtcgcg cccgcacggg aacgcgatca acgggcagct
     3301 ggtcgatgat ctgacccggg tctgcggaga gctggccgcc gatccgacga tccgcgggat
     3361 gatgctggcc gcgcgcggcc gcatcttctg tccgggtctc gacctgcacg agctga
//
LOCUS       DASPGN010000223        13963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_389271, whole genome shotgun sequence.
ACCESSION   DASPGN010000223 DASPGN010000000
VERSION     DASPGN010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389271"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VD788_06325"
     CDS             <1..755
                     /locus_tag="VD788_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75921.1"
                     /translation="ANPDPAGGDAPPAGAPGRVVLDLRHADFDLRAAGEGEGLGVRAS
                     YDEQSYTLLERFEPAAGAEWEYRVEFRRTGLGLLTGLKELLGGTRPKVEVRLPADRPL
                     ALDVDVQQSALTADLGGLWLTRAGVRVKQGAIDLDVSTPLRAPIDELSFNVSMGGVVL
                     ESLGNASPESLIVELHMGGGDIDLRGRWVNDAHVRIDTSMGGAQLRLPRDVSITGLER
                     GLSRVEPDAEIARPTLQIEIDPADLETIEIID"
     gene            complement(765..2438)
                     /locus_tag="VD788_06330"
     CDS             complement(765..2438)
                     /locus_tag="VD788_06330"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXV75922.1"
                     /translation="MSSSSNRLPIAIVLLLICPIVAVAHAAEIHVDASNMSGMEDGSP
                     AFPYTTIQQGIVHAAAGDKVRVAAGVYKEAVLMTDGVSVIGAGWRTTFIDATGLSASA
                     VVFDATRLSPLLSGFTITGGEGDQRSEIGDDPVTFGGGILILDSSPLILDNRIVANVV
                     EEGHCFGGGIYVSAQNSAPQIRGNIISGNTAESLTIPGNGRGGAIFASVKDGSVTIEQ
                     NVIETNRAIDGGGIVIENLSGAAATIRRNRLIGNRGKFGAAVWLMDADDSTTEVANNL
                     FVDNGSAQAGARGAGVLAFSSANGFFRIINNTFADNALDQGDGAALALDDSQAIVTDA
                     LVANNIVYQNEALTGGGIEYTAFGGTIRSNALFANAGGDLYEAGGSTATLIGNVVADP
                     GLAGTAINPYRLSSTSPLIDSAADDVSPSDDLDGLSRPFDGDGDLTAAADFGASEFPA
                     RTIDGVRFLSSVEIEWDAELADVSYQLYRGSLAVLRATGNYTQDPISEPLAARDCDLL
                     PSDLPVVDGFAPPPSVALFYLVSLEIGAYTGGLGLGPDGYERPNAFTCP"
     gene            complement(2505..4622)
                     /locus_tag="VD788_06335"
     CDS             complement(2505..4622)
                     /locus_tag="VD788_06335"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HXV75923.1"
                     /translation="MRIRRVLTTSVLVGVLALGAAAAKDPEVSTDPVPPVSRGDVFQR
                     IDPELSKAQSLVTRADQLLAEGRQDDAIELLHRVVAMELPAMRESDKLVAEAYLRLGD
                     VYGDPTRAPLKAVVFYGSAIERLSMDDDAVVISETLRRISSIYLSAGDEAASARIEGM
                     LVDIATGNPPAEFGLVPSDPGATTRDAGDDTCDMSIAVAVPHAEQMSINPAFDENWRD
                     FTLTEPRVVRIETLDADTLKDTVLELWDGCPGTSIDFDDDGGPGLLSLIESDCLEPGT
                     YYVEVRAFSAGTPQNFTLSITDEACPTPPLPDQYEPDNEPAQAGKIGFRNNGNGEGNQ
                     NGRDNKQIQRHSIFPAPDLDYVYFGLSRNNWVNIETLGVDGGNPDTVIGLTNEGGVLH
                     AVNDDRAAGQFTSQLRACLPAGNWFVPILPFFFDDEFDYDVLVDVEGSCLFETEPNGQ
                     CSLATPITLGEVWNGIHAPQGLTFETDWFSFEIEETSFVSIETSGYDDFDVDTYLELY
                     NACGGSLLDSDDDGGPGFTSRIDILLDPGTYYLLNRLSPIALASGVTYPYSLTITASE
                     PPLAEIEPNNSCGQANAVELGDSVEASISPIGDYDSFLLSVPSEGLIDIETTGGSGDT
                     VLSIADSAGNVQIGCDDDDGEGFFSKWSCCLPAGDYCVSVKDWGNNSTISSYTINFTD
                     LGSCTPNEPLVCDIAMVAQCSPF"
     gene            complement(4797..5078)
                     /locus_tag="VD788_06340"
     CDS             complement(4797..5078)
                     /locus_tag="VD788_06340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004934175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV75924.1"
                     /translation="MIKARARHILVESAASAENLIKQLESGADFAELARRHSSCPSGK
                     EGGDLGEFQPGQMVREFDDVVFRREIGKPHGPVKTEFGFHVIEILSRTG"
     gene            5239..5835
                     /locus_tag="VD788_06345"
     CDS             5239..5835
                     /locus_tag="VD788_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV75925.1"
                     /translation="MSDPDRALARKAGRGDRDALLTLYERHKGRLFGYLRRALRDRTL
                     AEDVFQEVWIKVMQGIELYRPSRATFRAWLFRIAANAAVDRVRREAVRAADPLDAPLE
                     TGEGRVVDRVASPRVAPDREGEGHLFGEALDRALGKLREGQRQAVLLRHQQGLSYPEI
                     AVALGVREGTAKTMVHRGVMRLRDELADWAPAGSSDAD"
     gene            5825..6298
                     /locus_tag="VD788_06350"
     CDS             5825..6298
                     /locus_tag="VD788_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75926.1"
                     /translation="MRTDAMRPGRDCGGLQRKLTEDAGAVGAAARRHLRDCRSCREFA
                     GRIEALSRELARTREIQPPPSLDRRLRSMLAEHRRVARALLRPAIAAGIGVAALLAFT
                     LWVAASLAQAGAGESGPLIAVVLAWAYLALSAAATMPVLLLAGRRTPARAAEARS"
     gene            6295..6834
                     /locus_tag="VD788_06355"
     CDS             6295..6834
                     /locus_tag="VD788_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXV75927.1"
                     /translation="MTPAGTGGRLRPPVAIVLAIAAFFLAALWMRGCIGGPLFDRASH
                     PFMLPWLGFYGPLGIWGLLQAALAVWVGLDAGKRGLNGVLWGLLVFFTGIVGLIVYLL
                     VGPAMQTQPTGDAPAATAATAGGRRCGSCATQVAPDFKVCPYCGTSLACGRCGKPVGG
                     DWKVCPYCTGPLDAPGSAP"
     gene            complement(6859..8886)
                     /gene="fusA"
                     /locus_tag="VD788_06360"
     CDS             complement(6859..8886)
                     /gene="fusA"
                     /locus_tag="VD788_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXV75928.1"
                     /translation="MAKIEATRNIGIVAHIDAGKTTVTERFLHDSGKIHKVGEVHDGQ
                     SQMDWMPQEKERGITITAAATTFEWKGHELHLIDTPGHVDFTIEVERSLRVLDGAVVV
                     FCGVGGVEPQSETVWRQADKFHVPRIAFVNKMDRVGASFPDVVRQIRERLGARAVPLQ
                     LPLGAEDRFEGVIDLVRMKVVRFSGVAGDPGEEGEIPPEFAAGAAEERQRLIEAAADF
                     DDVLAEKFLADDEPEPDELIAAVRRGCVGLGLVPVLCGSALRNKGVRPLLDAVVDYLP
                     SPIDVPPVRGVDPRDPTKTIERTASDKQPLAALVFKVAMDEGRKSVFVRVFSGVLGAG
                     AEVWNARAETTERVARLFKVHAHKRERLERAGAGSIVAAAGLKLATTGDTLCATAQPI
                     LLERIDAYEPVISIAIEPRSQAARERLDFALGKMVEEDPTFRVRQDEETGQTLISGMG
                     ELHLDVIVDRLHREYGVEASVGKPQVVCRETIREEAEAEATFERELKEAALYGRVACR
                     VRPRGRGQGNRILSALDGAAEIRPAIARAALDGLEEGAQLGPEGFPLEDIEATLLSLA
                     FRDDAQPEVGVKVAAAEALRSSVAKASPLIMEPIMDVEIVCAEEHLGGVLGDLRQRRA
                     HVQHVGDRGSAKLIDARVALRHMFGYSTDLRSLTKGRATFSMSFHAYDALA"
     gene            complement(8956..9828)
                     /locus_tag="VD788_06365"
     CDS             complement(8956..9828)
                     /locus_tag="VD788_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75929.1"
                     /translation="MSKHTTILSIALVVGLGAGLPVPASSPTVAPIGDSNTQQLPPDP
                     DDSSCPHSIIEGPDPIGAATECVKNPKDNRETNAMVDLPHVGQDPLSGKPFLVWSEPN
                     AGDRDIAFRRWGGSGWEPIEYLTSTADDERDPAAYASPDGSISVVWWSPTRGVELSRR
                     DAESGVWADPIPIGASGARPTIARVDQRILVAFERETEDGGTLIVAAAETTPGQFDET
                     VLAHDPGRAGAVELHVGGERVWADWRASDGRIAYSELNGGGWLAPRKLSIAGPTWEAE
                     RSARDAVRLELLSR"
     gene            complement(9825..11159)
                     /locus_tag="VD788_06370"
     CDS             complement(9825..11159)
                     /locus_tag="VD788_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXV75930.1"
                     /translation="MISANIHDSITAICGGRLLERKPTSDPDGRGRRKSPDSSKNGKE
                     RPGARGSSRLAAAQQSIGTFLRATRTAQKLTQEQVAAMTRDTPWHLSRAAISAIERGQ
                     NFPGMEAMLALSNVLYVDPKELIERARLSTTVPVDVTDLSLEQLGRRAARYFWSGDFR
                     KALSVYDAMLEKLALDPPADVGRELANLEIRRATTLKRAGALLSAIATAERAISLSAE
                     IPQVHAEAYILLADLQCQRGHLPLAGDAAKKAIELSRTLDPRTQGWAWMTEAHVRFLS
                     ADYQQARNAFLEARKQATAAGDEQHLTHIDGDVGMCLLALGQVAEARTWVARAVERAR
                     KQSQPALEASWLVELGKIALRQRNFEEAERFASSALRIARPREHVVTIFRAEWLQHNI
                     ARSTRPDDPDRLRIARLRKLYLHLDQHEGIEEVQVFKRTIMRKSAIEYGESR"
     gene            11273..12049
                     /locus_tag="VD788_06375"
     CDS             11273..12049
                     /locus_tag="VD788_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75931.1"
                     /translation="MPRSPVLMGLLLVAGIASAAQAPSLEELRAAVVRDPADENAHRE
                     LAIALHDAHRIDEAIAEFEWLARRSPTERSLLDLALAYGSGSRWDDAEATYRRILETS
                     PNHAIVLHNLGNTAWRRGDPETAIEYYVKAIKAKPDYLLAHARLGDALTQAERYRDAY
                     RAYESVLELEPQTAAEADAYYDALYHMAALDLQMGAHERAGMMLAELIRADPDHPKAY
                     YAYGQVLMFMGREEDAQKAFAEHQRIQANQAPSSTAAHGD"
     gene            12059..13750
                     /locus_tag="VD788_06380"
     CDS             12059..13750
                     /locus_tag="VD788_06380"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HXV75932.1"
                     /translation="MAMIARVTALSACLFVLASASTTGPLHFVDATAGAGIDYVNVCG
                     APLGKKGWLNEGMGAGAAWLDYDADGKLDLYVVNGSAFDRKPGAGEPNRLFRGDGTGK
                     FADVSVKAGVADRGWGYGVTVGDYDNDGDADLFVTNMGPNVLYRNRGDGTFEDVTARA
                     GVAGDDVWSSSAAFFDMDRDGDLDLYVGNYMVCSPTTVARAGSPEAIAANCVYYGVAV
                     PCGPMSQVPIQDVLYRNDGDGTFSDVTRAAGLWLDTARYALGVVTFDYDNDGDQDVYV
                     ANDSVANSLWENDGKGRFTDVGLIKLAAFNAHGRAQAGMGADAGDYNGDGWMDLVVTN
                     FSQDLNTIYKNLAGKFFIDDSNLAGMNVTHMFLSWGVGFHDFDLDGDLDLFIANGHVY
                     PMLDGKQVGTNYRQPNHLFLNDGDRFVESAARSGPGLALERSFRGAAFADYDDDGDVD
                     VFLTALDDAPQLLRNDSPRAGRHYLTLRLIGQTSNRDGVGARVTTTIGSSQLIRERKG
                     GGSYQSASDPRVHFGVGSATVVDRIEVRWPSGVADVLSDVPVDRELTIVEGQSRD"
     gene            13774..>13963
                     /locus_tag="VD788_06385"
     CDS             13774..>13963
                     /locus_tag="VD788_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75933.1"
                     /translation="MGSAVGSCLALACLSAPLIGPSADGAAPEQVVLRLADRSWGLRI
                     APPGFEWGAAQSARNGTAV"
BASE COUNT         2121 a         4748 c         4819 g         2275 t
ORIGIN      
        1 cggccaaccc cgatcctgcg ggcggcgacg cgccgcccgc aggcgccccc ggccgggtcg
       61 tgctcgacct gcggcacgcg gatttcgatc tccgcgccgc gggcgagggc gagggcctgg
      121 gcgtgcgggc gagctacgac gagcagtcgt acacgctgct cgagcggttc gaacccgcgg
      181 ccggcgcgga gtgggagtac cgcgtcgagt ttcgccggac cgggctgggt ctgttgaccg
      241 gtctgaagga gctgctcggc ggcacgcgcc ccaaggtcga ggtccgcttg ccggcggacc
      301 ggccgctggc gctcgacgtc gacgttcaac agagcgcgct gaccgccgat ctcggcggcc
      361 tgtggctgac ccgcgccggc gtccgcgtca agcagggggc gatcgacctc gacgtgtcca
      421 cgccgttgcg tgcgccgatc gacgagctgt cgttcaacgt ctcgatgggc ggcgtcgtgc
      481 tcgagtcgct gggcaacgcg tcgcccgagt cgctgatcgt cgagctccac atggggggcg
      541 gcgacatcga tctgcgcggg cggtgggtca acgacgccca cgtcaggatc gacacctcga
      601 tgggtggagc gcagctccgg ctgccgcgcg acgtgtcgat caccggtctc gaacgcgggt
      661 tgtcccgcgt cgagccggac gccgagatcg cccggcccac gctgcagatc gagatcgacc
      721 cggccgatct cgagacgatc gagatcatcg attgacgcga tcggtcaggg acaggtgaag
      781 gcgttcggcc gctcgtagcc gtcgggcccg agaccgagac cgccggtgta cgcgccgatc
      841 tcgagtgaaa cgaggtagaa cagcgccacg ctcggcgggg gggcgaagcc gtcgacgacg
      901 ggcagatcgg acggcagcag gtcgcagtcg cgtgcggcca gcggctcgct gatcggatcc
      961 tgcgtgtagt tgccagtcgc gcgcagtacg gccaacgaac cgcggtagag ctggtaggac
     1021 acgtcggcca gctcggcgtc ccattcgatc tcgaccgagg acaggaagcg caccccgtcg
     1081 atcgtgcgcg ccggaaactc ggatgcgccg aagtcggcgg ccgcggtcag gtcgccgtct
     1141 ccgtcgaacg gtcgactgag gccgtcgagg tcgtcggacg gcgacacgtc gtccgccgcg
     1201 gaatcgatca gcggcgaggt cgaggacagc cgataggggt tgatcgccgt gccggccagc
     1261 cccggatcgg ccaccacgtt accgatcaag gtcgcggtgc tgccaccggc ctcgtacaga
     1321 tcgccgccgg cgttggcgaa caacgcgttg ctgcgaatcg tcccgccgaa ggcggtgtac
     1381 tcgatgccgc ccccggtcag cgcctcgttc tggtagacga tgttgttggc gaccagcgcg
     1441 tcggtcacga ttgcctgcga atcgtcgagc gccagcgccg ccccgtcgcc ttggtcgagc
     1501 gcgttgtccg cgaacgtgtt gttgatgatg cggaaaaagc cgttcgccga ggagaacgcg
     1561 agcaccccgg ccccgcgcgc gccggcctgc gcggagccgt tgtcgacgaa caggttgttg
     1621 gcgacctcgg tggtcgagtc gtcggcgtcc atcaaccaca ccgccgcacc gaacttgccg
     1681 cggttgccga tcagcctgtt gcgccggatc gtcgcggccg ccccgctcag gttctcgatc
     1741 acgatcccgc cgccgtcgat cgcccgattg gtctcgatga cgttctgctc gatcgtcacg
     1801 ctgccgtcct tgaccgaggc gaagatcgcg ccaccccggc cgttgccggg gatcgtcagc
     1861 gactcggccg tgttgccgct gatgatgttg ccgcggatct gcggcgccga gttctgggcg
     1921 ctgacgtaga tgccgccgcc gaaacagtgg ccctcttcga cgacgttggc cacgatgcga
     1981 ttgtcgagga tcaatggcga cgaatcgagt atcaggatgc cgccgccgaa cgtgaccggg
     2041 tcgtcgccga tctccgaccg ttggtcgccc tcgcccccgg tgatggtgaa tccgctgagc
     2101 agcggactga gccgcgtcgc gtcgaagacg accgcgctcg ccgacagtcc ggtcgcgtcg
     2161 atgaacgtcg tgcgccagcc ggcgccgatc acgctcaccc cgtccgtcat cagcacggct
     2221 tccttgtaga ccccggcggc gacccgcacc ttgtcgcccg cggcggcatg gacgattccc
     2281 tgttggatcg tcgtgtacgg aaaagccggg ctgccgtcct ccattccgct catgttcgac
     2341 gcgtcgacgt ggatctcggc ggcgtgggcg accgccacga tcgggcagat caaaagcagg
     2401 acgatcgcga tcggaagtcg attgctcgac gagctcatcg gccggccccg cggaaaaacg
     2461 acgggggagg ggttcccccc tcccccgtcg gttgagtcaa cgaactagaa cgggctgcac
     2521 tgggccacca tcgcgatgtc gcacaccaac ggctcgttgg gcgtacagct accgagatcg
     2581 gtgaagttga tcgtgtagct gctgatcgtg ctgttgttgc cccaatcctt gacggagacg
     2641 cagtagtcac ccgcgggcag gcagcaactc cacttcgaga aaaagccttc gccgtcgtca
     2701 tcgtcgcagc cgatctgcac gttgccggcg gagtcggcga tgctcagcac ggtgtccccg
     2761 ctgccgccgg tcgtctcgat gtcgatcaga ccctcggacg ggacgctgag caggaagctg
     2821 tcgtaatcgc cgatcgggct gatcgacgcc tcgaccgaat cacccagctc gaccgcgttg
     2881 gcctgaccgc acgagttgtt cggttcaatc tcggccagcg gcggctcgct cgccgtgatc
     2941 gtcagcgagt agggataggt cacgccgctc gccagtgcga tcggcgacag gcggttgagc
     3001 aggtagtacg ttcccgggtc gagcaggatg tcgatccggg aggtgaatcc cggcccgccg
     3061 tcgtcgtcgg agtcgagcag gctgccgccg caggcgttgt acagctcgag ataggtgtcg
     3121 acgtcgaagt cgtcgtaacc cgaggtctcg atcgaaacga acgaggtctc ctcgatctcg
     3181 aaactgaacc agtcggtctc gaaggtcaga ccctgcggcg cgtggatccc gttccacacc
     3241 tcgccgagcg tgatcggcgt ggcgaggctg cactggccgt tcggctcggt ctcgaacaga
     3301 cagctcccct cgacgtcgac cagcacgtcg taatcgaact cgtcgtcgaa gaagaacggc
     3361 aggatcggaa cgaaccagtt accggccggc aagcaggctc gcagctgtga ggtgaactgg
     3421 ccggcggcgc ggtcgtcgtt gaccgcgtgc agcaccccgc cttcgttggt caagccgatg
     3481 accgtgtcgg ggttgccacc gtcgacaccg agagtctcga tgttgaccca gttgttgcgg
     3541 ctgagaccga aatagacgta gtccagatcg ggtgcgggga agatcgagtg gcgctggatc
     3601 tgcttgttgt cgcgtccgtt ctggttgcct tcgccgttgc cgttgttgcg gaagccgatc
     3661 ttcccggcct gcgccggctc gttgtcgggt tcgtactgat ccggcaacgg cggtgtcggg
     3721 caggcctcgt cggtgatgct cagcgtgaag ttctgcgggg taccagcgct gaacgcccga
     3781 acctcgacgt agtaggtccc cggctcgagg cagtcgctct cgatcaacga caacagcccc
     3841 gggccgccgt catcgtcgaa gtcgatcgag gtgccggggc agccgtccca cagctcgagc
     3901 acggtgtcct tgagcgtatc ggcgtcgagc gtttcgatcc tgacgacccg cggctcggtc
     3961 agggtaaagt cacgccagtt ctcgtcgaac gccgggttga tgctcatctg ctcggcatgc
     4021 ggcactgcga cggcgatcga catgtcgcag gtatcgtcac cggcgtcgcg tgtcgtcgcg
     4081 ccgggatcgc tcggcacgag accgaactcg gcgggcgggt tgccggttgc gatgtcgacg
     4141 agcatcccct cgatccgagc cgaggcggcc tcgtcgcccg cggacaggta gatcgagctg
     4201 atgcggcgca gcgtttctga gatcaccacc gcgtcgtcat ccatcgacag ccgttcgatg
     4261 gcggagccgt agaacacgac ggccttgagc ggagcgcggg tcggatcacc gtagacatcc
     4321 cccaggcgga ggtacgcctc ggccaccagc ttgtccgact cgcgcatcgc gggcagttcc
     4381 atcgccacga cgcggtggag cagttcgatc gcgtcgtcct gtcgtccctc ggccagaagt
     4441 tggtcggcac gcgtcacgag cgactgggct tttgaaagct cggggtcgat tcgctggaac
     4501 acgtcaccgc ggctgaccgg cggcaccggg tccgtggaga cttccgggtc tttcgccgcc
     4561 gccgctccaa gggcgagcac gccgacgaga accgaggtgg tcagaacacg ccttatcctc
     4621 atggtcactc ctttttctcg ggggggaggg cgttgcctgt cccgacacaa atgtgtcgtt
     4681 cgcgtcggct caattatgta cggcgagaat cgcgaagtga aggcggaaat atcgtgaaat
     4741 cgccgccaaa cccgactatc cgggtcccga tcaccggatc ggcgggccgc agccgctcag
     4801 ccggtgcggc tcaggatctc gatcacgtgg aagccgaatt cggtcttgac cggaccgtgg
     4861 ggtttgccga tctctcggcg gaagacgacg tcgtcgaact cccgcaccat ctgaccgggc
     4921 tggaactccc ccagatcccc gccctctttg cccgacgggc agctcgagtg ccggcgcgcc
     4981 agctcggcga agtcggcgcc agattccagc tgcttgatca ggttctccgc cgaggcggcc
     5041 gactcgacca ggatgtgacg ggctcgggcc ttgatcatcg ctcggactcc ccccgcgtcg
     5101 gcgggctccg tgcgcggggc tatccggccg agcacggaat cagcgtgagc gaggcggatt
     5161 atacgaccgc ggcaacgatc cgtcgaaacg gctgcgacct gcggtgcgta ccagacgggg
     5221 ggaggcgcgt cgcgaacgat gtccgatcct gaccgggcgc tggccaggaa ggccgggcgc
     5281 ggcgaccgcg acgcgctcct gacgttgtac gaacgacaca aggggcgctt gttcggttac
     5341 ctcagacgcg cgttgcgaga tcggaccctc gccgaagacg tgttccagga ggtctggatc
     5401 aaggtgatgc aagggatcga gctataccgg ccgagccggg cgaccttccg ggcctggctg
     5461 ttccgcatcg ccgccaacgc ggcggtcgat cgcgtgcgac gcgaggcggt gcgggcggcc
     5521 gatccgctcg acgcgccgct cgagaccggc gagggacgcg tggtcgaccg cgtggcgtcc
     5581 ccacgggtcg cgcccgatcg cgagggcgag ggccatctgt tcggcgaggc gctcgaccgt
     5641 gcgctcggca agctgcgtga gggccagcgg caagccgtgc tgttgcgcca ccagcagggg
     5701 ctgagttatc ccgagatcgc ggtcgcgttg ggcgtccgcg agggaacggc caaaacgatg
     5761 gttcaccgtg gtgtgatgcg attgcgcgac gagctggcgg actgggctcc ggcgggatcg
     5821 agcgatgcgg actgacgcga tgcgacccgg acgcgactgc ggcggccttc agcggaaact
     5881 gacggaggac gccggggcgg tcggggccgc ggcgcggcgg cacctgcgtg attgtcggtc
     5941 ctgccgcgag ttcgccggcc ggatcgaggc gttgagccgc gagctggcgc ggacacgtga
     6001 gatccagcca ccgccgtcgc tcgatcggcg cttgcgctcg atgctcgccg agcatcgacg
     6061 cgtcgcgcga gcgctgctgc gcccggcgat cgcggccggt atcggtgtcg cggcgttgtt
     6121 ggcgttcacg ctgtgggtcg cggcctcgct ggcgcaggcc ggcgccggcg agtcgggacc
     6181 gctgatcgcg gtcgtcctcg cttgggccta tctcgcgttg agcgccgccg cgacgatgcc
     6241 ggtgctgctg ctcgccgggc ggcgcacgcc ggcgcgcgcc gccgaggccc ggtcgtgacc
     6301 ccggccggca ccggcgggcg gctccgtccc ccggtcgcga tcgttctcgc gatcgccgcc
     6361 ttcttcctcg cggcgctgtg gatgcgcggc tgcatcggcg ggccgctgtt cgatcgcgcg
     6421 agccacccgt tcatgctccc gtggctcggc ttctacggac cgctcggtat ctgggggttg
     6481 ctgcaggcgg cactcgcggt ctgggtcggt ctcgatgccg gcaagcgcgg gctgaacggc
     6541 gtgctgtggg gcctgctcgt gttcttcacc gggatcgtcg ggctgatcgt ctacctgctc
     6601 gtcggaccgg cgatgcagac gcaaccgacc ggcgacgccc cggcggcgac ggccgcgacc
     6661 gccggcggcc ggcgctgcgg atcgtgcgcc acgcaggtcg ccccggactt caaggtctgc
     6721 ccgtactgcg gcacctcgct cgcatgcggg cgctgtggca aaccggtcgg cggcgactgg
     6781 aaggtctgtc cgtactgcac cggaccgctc gacgcaccgg gcagcgcccc gtgacggagg
     6841 ctcgcgcccc cggcaacctc aagccagagc gtcgtaggcg tggaacgaca tgctgaaggt
     6901 cgcgcgcccc ttcgtcagcg agcgaagatc ggtcgagtaa ccgaacatgt gccgcagcgc
     6961 gacgcgcgcg tcgatcagct tggccgagcc ccgatcgccg acgtgctgca cgtgcgctct
     7021 gcgctggcgc aggtcgccga gcacaccgcc caggtgctcc tcggcgcaga cgatctcgac
     7081 gtccatgatc ggctccatga tcagcggcga ggctttggcc accgacgagc gcagcgcttc
     7141 ggccgcggcc accttgaccc cgacctcggg ttgcgcgtca tcacggaagg cgagcgacag
     7201 cagcgtcgct tcgatgtcct cgagcgggaa gccctcgggt ccgagctggg ccccctcctc
     7261 gagcccgtcg agcgccgcgc gggcgatcgc cggacggatc tcggccgcgc cgtcgagcgc
     7321 ggacaggatg cggttgccct gcccgcgccc gcgcgggcgg acacgacagg cgacccgacc
     7381 gtagagcgcg gcttccttga gctcgcgctc gaaggtcgcc tcggcctccg cctcctcgcg
     7441 gatcgtctcg cgacagacga cctgcggctt gccgacgctg gcctcgacgc cgtactcgcg
     7501 gtgcagccga tcgacgatca cgtcgaggtg cagctctccc attccgctga tgagcgtctg
     7561 cccggtctcc tcgtcctgac gcacgcgaaa ggtcggatcc tcttcgacca tcttgccgag
     7621 cgcgaaatcg agccgctcgc gcgcggcctg gctgcgcggt tcgatcgcga tcgagatcac
     7681 cggctcgtag gcgtcgatcc gctcgagcag gatcggctgc gccgtggcgc acaaggtgtc
     7741 gccggtggtc gccagcttga gcccggcggc ggcgacgatg ctcccggcgc cggcgcgctc
     7801 cagccgctcg cgcttgtgcg catggacctt gaacagccgc gccacgcgtt cggtggtctc
     7861 ggcgcgcgcg ttccacacct cggcgcctgc tccgagcacg ccgctgaata cgcggacgaa
     7921 gacgctcttg cggccctcgt ccatcgccac cttgaacacg agagccgcga gcggctgttt
     7981 gtcgctggcg gtgcgctcga tcgtcttcgt cgggtcacgc gggtcgacgc cgcgaacggg
     8041 agggacgtcg atcggcgagg gcaggtagtc gacgaccgca tcgagcaacg gacgcacccc
     8101 cttgttgcgc agcgcgctgc cgcacagcac gggcacgagc cccaaaccga cgcacccgcg
     8161 acgcaccgcc gcgatcagct cgtcgggttc gggctcgtcg tccgccagga acttctcggc
     8221 cagcacgtca tcgaagtcgg ccgcggcctc gatcaaccgt tgtcgttcct cggccgcgcc
     8281 cgcggcgaac tcgggcggaa tctcgccctc ctcgcccgga tcccccgcca ctccgctgaa
     8341 ccgcaccacc ttcatccgca ccaggtcgat gacgccctcg aaccgatcct ccgctccgag
     8401 cggcagctgc agcgggacgg cgcgcgcgcc cagccgctcc cgaatctggc gtacgacgtc
     8461 gggaaagctg gccccgacgc gatccatctt gttgacgaag gcgatgcgag gcacgtggaa
     8521 cttgtcggcc tgccgccaga cggtctccga ctgcggctcg accccgccga ccccgcagaa
     8581 aacgacgact gcgccgtcga gcacacgcag actgcgctcg acctcgatcg tgaagtcgac
     8641 atggccgggc gtgtcgatca ggtggagctc gtggcccttc cactcgaagg tcgtcgcggc
     8701 cgcggtgatg gtgatgcccc gctctttttc ctgcggcatc cagtccatct gcgactggcc
     8761 gtcgtggacc tcgccgacct tgtggatctt gcccgagtcg tgcaggaacc gctcggtgac
     8821 cgtcgtcttg ccggcgtcga tgtgggccac gatgccgatg ttgcgcgtcg cctcgatctt
     8881 ggccatcgcg caacaggata gcgaacgccg cgagtggcgg acagtgccgg cgcgcgcgag
     8941 cgcgcccacc cgccgtcagc gcgagagcaa ctcgaggcga acggcgtcgc gcgccgagcg
     9001 ttccgcctcc caggtcggcc cggcgatcga cagcttgcgc ggtgcgagcc agcccccccc
     9061 gttcagctcg gagtaggcga tccgtccgtc gctcgcacgc cagtcggccc acacgcgctc
     9121 gccgccgacg tgcagctcga ccgcccccgc ccgtcccggg tcatgcgcca gcactgtctc
     9181 gtcgaactgg cccggcgtgg tctcggccgc ggcgacgatc agcgtgccgc cgtcttcggt
     9241 ctcgcgctcg aaggcgacga ggattcgctg gtcgacccgc gcgatcgtcg gacgcgcgcc
     9301 gctcgcgccg atcggaatcg gatcggccca gacaccggat tccgcgtcgc gccgcgagag
     9361 ctcgacgccc cgcgtcggcg accaccagac gaccgagatc gaaccgtcgg gcgaggcgta
     9421 tgccgccgga tcgcgctcgt cgtctgcggt cgaggtcaag tactcgatcg gctcccagcc
     9481 ggagccgccc cagcggcgga aggcgatgtc gcgatcgccg gcgttcggtt cggaccagac
     9541 gaggaacggc ttgccgctga gcggatcctg gccgacgtgc ggcaggtcca ccatcgcgtt
     9601 ggtctcgcga ttgtccttcg ggttcttcac gcactcggtc gcggcgccga tcggatcggg
     9661 gccctcgatg atgctatgcg ggcagctcga atcgtccggg tcgggtggca gctgctgcgt
     9721 gttcgagtcg ccgatcggcg cgaccgtcgg cgacgaggcc ggcaccggca ggccggcgcc
     9781 gagacccaca accaacgcaa tggacagaat cgtggtgtgc ttgctcatcg actctccccg
     9841 tactcgatcg ccgattttcg catgatggtc cgcttgaaga cctggacctc ttcgatgcct
     9901 tcgtgctgat cgaggtgtag atagagcttc cgcaggcgag cgattcgaag acggtcgggg
     9961 tcgtccggcc gcgtactgcg cgcgatgttg tgctgcagcc attccgctcg gaagatcgtc
    10021 acgacgtgct cgcgtggtct cgcgatccgc aacgccgacg aggcgaagcg ctccgcctcc
    10081 tcgaaattcc gttggcgcaa ggcgatcttg ccgagctcga cgagccagct cgcctcgagc
    10141 gccggctggc tctgcttccg cgcgcgctcg acggcgcgtg cgacccacgt gcgtgcctcg
    10201 gccacctgtc ccagagccag caagcacatg ccgacgtccc cgtcgatgtg cgtcagatgc
    10261 tgctcgtcgc ccgccgcggt cgcttgcttg cgcgcctcga gaaacgcgtt ccgcgcctgc
    10321 tggtagtcag cggaaaggaa gcgcacgtgg gcctcggtca tccaggccca gccctgggtc
    10381 cgcggatcga gcgtcctcga cagctcgatc gctttcttcg ccgcatcacc ggcgagcggc
    10441 aagtggccgc gctgacactg gaggtcggcc aacaagatgt aggcctcggc gtgaacctgc
    10501 ggaatctcgg cgctgagcga gatcgcgcgc tcggcggtgg cgatcgccga caacagcgca
    10561 cccgcgcgct tcaacgtcgt cgcccggcgg atctcgaggt ttgccagctc gcggccgacg
    10621 tcggccggcg ggtcgagcgc cagcttttcg agcatcgcgt cgtagaccga caacgccttg
    10681 cgaaaatccc cgctccagaa gtagcgcgcc gcgcggcgac cgagctgctc gagcgacagg
    10741 tcggtcacgt cgaccgggac cgtggtcgac agccgcgcgc gctcgatcag ctccttcgga
    10801 tcgacgtaca gcacgttcga cagcgcgagc atcgcctcca tccccgggaa gttctggccg
    10861 cgctcgatcg cgctgatcgc ggcacgcgac aggtgccacg gcgtgtcgcg cgtcatcgcg
    10921 gcgacctgct cctgcgtcag cttctgtgcg gtacgagtcg cgcggaggaa ggtgccgatc
    10981 gactgttggg ccgcggcgag acgggacgac ccgcgggccc ccggtcgctc cttgccgttt
    11041 ttcgaggagt ctggcgattt tcgccgaccg cgcccatcgg gatcgctcgt cggtttgcgc
    11101 tccaacagcc gccccccgca gatcgccgtg atgctgtcgt gaatgttggc ggatatcacg
    11161 ctaacccgca gtcttgcgtg ctgtcaacca gacccgcccg aatcgctcac gttcttatat
    11221 ggaccaaaat ggcaaacttg ggtaggttat gggtggcttt cgaggaaaaa agatgccgag
    11281 aagcccggtt ctgatgggtt tgctgctcgt cgcgggcatc gcgtcggccg cccaagcgcc
    11341 ctcgctggaa gagttacgcg ccgcggtcgt ccgggacccg gccgacgaga acgcgcatcg
    11401 ggagctcgcg atcgcgctgc acgacgccca tcgcatcgac gaggcgatcg cggagttcga
    11461 atggctggcc cggcgctcgc cgaccgagcg cagtctgctc gacctggcgc tggcctacgg
    11521 cagcgggtcg cgctgggacg acgccgaggc gacctaccgg cgcatcctcg aaacgagccc
    11581 gaaccacgcg atcgtgctgc acaatctcgg caacaccgcg tggcggcggg gcgatcccga
    11641 gaccgcgatc gagtactacg tcaaggcgat caaggccaag cccgactacc tgttggccca
    11701 tgcacgcctg ggcgacgcgc tgacccaggc cgaacgctat cgggacgcct atcgagcgta
    11761 cgagagcgtg ctcgagctcg agcctcagac cgcggcggaa gccgacgcgt actacgacgc
    11821 gctgtaccac atggcggccc tcgatctgca gatgggagcc cacgagcgcg cggggatgat
    11881 gctggccgag ctgatccgag ccgatcccga ccatcccaag gcgtactacg cgtacggcca
    11941 ggtgctgatg ttcatggggc gcgaggaaga cgcgcagaag gcgttcgccg agcaccagcg
    12001 gatccaggcc aaccaggctc cgtccagcac ggccgcgcat ggcgattgac gtgtcccgat
    12061 ggcgatgatc gcgcgggtca ccgcgctctc ggcctgtctg ttcgtcctcg cctccgcctc
    12121 caccaccggg ccgctgcact tcgtcgacgc gaccgcggga gcggggatcg actacgtcaa
    12181 cgtgtgcggc gcgccgctgg gcaagaaggg ctggctcaac gaggggatgg gtgcgggcgc
    12241 ggcctggctc gactacgacg cggacggcaa gctcgacctg tacgtggtca acggttccgc
    12301 gttcgatcgc aagccgggag cgggtgagcc gaaccgcctg ttccgtgggg acggcacggg
    12361 caagttcgcc gacgtgtcgg tcaaggccgg cgtcgccgac cgcggctggg gctacggcgt
    12421 caccgtcggc gattacgaca acgacggcga tgccgatttg ttcgtcacca acatgggacc
    12481 caacgtgctg taccgcaacc gtggggacgg cacgttcgag gacgtgacgg cccgggccgg
    12541 cgtcgccggg gacgacgttt ggtcgagctc cgcggccttc ttcgacatgg atcgtgacgg
    12601 cgacctcgat ttgtacgtcg gcaactacat ggtctgttcg ccgacgacgg tcgcgcgggc
    12661 aggcagcccc gaggcgatcg ccgccaactg cgtctattac ggcgtcgcgg tgccgtgcgg
    12721 gccgatgagc caggtgccga ttcaggacgt gctctatcgc aacgacggcg acggcacgtt
    12781 cagcgatgtc acgcgcgcgg ccggtctgtg gctcgacacg gcgcgctacg cgttgggcgt
    12841 cgtcaccttc gactacgaca acgacggcga ccaggacgtc tacgtcgcca acgacagtgt
    12901 cgccaactcg ttgtgggaga acgacggcaa gggacgcttc accgacgtcg gtctgatcaa
    12961 gctggccgcg ttcaacgcgc acgggcgggc gcaggccggg atgggcgccg acgccggcga
    13021 ctacaacggc gacggctgga tggacctggt ggtgaccaac ttctcgcagg acctgaacac
    13081 gatctacaag aacctcgccg ggaagttctt catcgacgat tcgaacctgg cggggatgaa
    13141 cgtgacccac atgtttctgt cgtggggcgt cgggttccac gacttcgacc tggacggcga
    13201 cctcgacctg ttcatcgcca acggccacgt ctacccgatg ctcgacggaa agcaggtcgg
    13261 caccaactac cggcagccga accacctgtt cctcaacgac ggcgaccgtt tcgtcgaaag
    13321 cgcggcgcgg tcgggcccgg gcctggcgct cgagcgttcg tttcgcggag ccgcgttcgc
    13381 ggactacgac gacgacggcg acgtcgacgt cttcctgacc gcgctcgacg atgcgcctca
    13441 actgctgcgc aacgacagcc cgcgtgccgg ccggcactac ctcacgctcc ggctgatcgg
    13501 tcagacctcg aaccgggacg gcgtcggggc gcgggtcacg accacgatcg gcagctcgca
    13561 gctgattcgc gagcgcaagg gcggcggttc gtaccagtcg gcgagcgacc cgcgggtgca
    13621 cttcggcgtc ggcagcgcga ccgtggtcga ccggatcgag gtgcgctggc cgagcggcgt
    13681 ggccgacgtg ctgtccgacg tgcccgtcga tcgcgagctg acgatcgtcg agggtcagag
    13741 ccgggactag ccggcggctg ctaagctgtc ggaatgggat ccgcggtcgg ctcgtgcctc
    13801 gccttggcct gtctgtccgc gccgctgatc ggtccgtccg cggacggggc agcgccggag
    13861 caggtcgtgc tgcgacttgc cgatcgttcg tgggggttgc gtatcgcgcc tccggggttc
    13921 gagtggggcg cggcgcagtc cgcacgcaac gggacggcgg tgt
//
LOCUS       DASPGN010000224         8141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1677704, whole genome shotgun sequence.
ACCESSION   DASPGN010000224 DASPGN010000000
VERSION     DASPGN010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677704"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VD788_06390"
     CDS             <1..451
                     /locus_tag="VD788_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75934.1"
                     /translation="LLSQLVLYLAAHPGNEPAMGTAVLIDYYGFTDEEKIDALAPHFD
                     SADPRLRDAIWEVLLTVDTPAGARSSVELVRQVEQALDGGAKAVATGDVLATLARDES
                     WWIRLYVAHVARRRPELVRPEVLARLREDEDPRVRRAADGGSSAEGR"
     gene            complement(448..1221)
                     /locus_tag="VD788_06395"
     CDS             complement(448..1221)
                     /locus_tag="VD788_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75935.1"
                     /translation="MKRVYVVGVGEVGRRLAGALERDGWQVARVTRRRGWPAAADHRD
                     PSPRIVAVREEALAGVLARLPADLRVRVVLVQNGFLEAVHDDLGETTRGLIWFTSKGA
                     FFRSLRPSVFRGAWAEPLVAALDRGGIDVVRIDDRDRFAREMILKGIWNCVVGLPLVA
                     HDVDLATYLREWHDELVDLVAECATAAAAHYGVSVDPRAALDRIEETTTELGWMRGGA
                     KALAWRNGAIVTFGRRHGIPTPVNDRLLRAATGTTPGGG"
     gene            1322..1951
                     /locus_tag="VD788_06400"
     CDS             1322..1951
                     /locus_tag="VD788_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75936.1"
                     /translation="MRRSSWRPVRAALALVVGLSAPVCDAATIVRSADLGELVAEAGS
                     AILADVVAVRYGRDERGLHGTWVTVRVADPIFGDGLPAAGGEYTFKSYGAPVTMPDGS
                     RVYIDGSPSYRPGDSLLLLLLPRSRWGFSGTAGLSQGVFRVRVDARGERTAVGMAGNA
                     GVFGERGLAGWLDPAARSGVPAAPDAPVPYAALRRAIADLARTAGKVAR"
     gene            1948..3471
                     /locus_tag="VD788_06405"
     CDS             1948..3471
                     /locus_tag="VD788_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75937.1"
                     /translation="MSGGHLRSLVATAGVLLALGWNPIEVRPDGPLRWETSAPVVWNP
                     DGGPLGMLDNAGATAMVQDGFAVWQAVPTATISFAVGPQIVDGSDAPVDVTADNFLEI
                     VNLGNPQNPVIFDNEQEIWAALGISTSVIGFAGALAASGPTITKGFVALQGDWIDGVT
                     VNPPDCGGQRCDPELNQVSQFLNIVVHEIGHFIGLGHAVVNRGVGSLAGCPAPGISVF
                     ATMLPFAHFSMETLHHDDVVGVSSLYPTAEFDTDFARISGRLFARDGAGPLDGANVVL
                     RPDTTDCALLYGGAQATQSGVNPAETGGAGTYRFAGLDPATGYTLHVSEIDDGGSYPI
                     GGSSPPPLGGPAEFHNGVDEDHFDPPDAPDDFELLLTGEAGSELNGRDLRVNNAGGPA
                     AIAVSGAGSLQLTKTLGGDPLLSWGPPCNADLVPDQTYVVYAGSLSSVGGVQDHLPLV
                     CDTGGAPSLEVTAGGGDRYYLVAPRIAGREGALATGSGGLLRTPVETCAEVRPDECP"
     gene            3554..4672
                     /locus_tag="VD788_06410"
     CDS             3554..4672
                     /locus_tag="VD788_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV75938.1"
                     /translation="MTPDGPARQPRPAMSFRLAACATVCAATVASGQDGPPPALVVTE
                     PVVHEELVEKIELIGSVAPIQDSLVAAEVEGRVALRSIDRGDQVDRGQLLVRLDAARL
                     EQELARVRALLVDVESQLELASIQERRARDLFEQEILSQGELDQALAARRSLDGRADS
                     TRAAIASIEVDLDRSTIRAPFDGIVTDVGVEIGEWVDRGAWIVRLTDLDTVEIRLEVP
                     EQYFRQLRRGASAPVAVDAYPGLTLDGRVFAVVPQADPEARTFPVLVRAGNPEGKIGS
                     GMLARVMLILTGGERVLTVPKDAIVRQAQQEMLWIVDGGVARPVSVRTGRGAGTRVEV
                     TGNLSPGDLVVVRGNERLSPGQPVIVETAHGDGRPSEN"
     gene            4677..7850
                     /locus_tag="VD788_06415"
     CDS             4677..7850
                     /locus_tag="VD788_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV75939.1"
                     /translation="MGIVRFAIDQPITVAVGAILVALFGLVSLNFVPIQLTPDIDRPR
                     ATVLTTWPGASPFEIEREIIDEQEEQLKGLDGLLKMESESQDGFGQIMLEFPVGTDPD
                     SILVRVSNRLEQVPTYPPDVDKPVIFTVDPGAQAMAWFMIYADDGYDIDPAKLSDYVD
                     DFIKPPIERVPGVAQVNLFGGREREMQVVVDPKRLAARGLTMSEVAAALAAENKDYSA
                     GDFREGKRRYVVRTAGDYRSPEEVENVIVATSGGAPVHVRDIGRVQLGYRKPTEFVRA
                     LGRPALAFNAQRAPGSNVLDVMARVRVVVERLNEELLRPQGLRLDIAYQETDYIDSAI
                     ALVRSNIFVGGALALIVLLLFLRSLSGTFVVGLSIPISMVATFLVMALAGRSINVISL
                     AGLAFAVGMVVDNCIVVLENVYRHRQMGKRRMVAALDGAVEVWGAVLASTLTTVAVFL
                     PVIFVRDEAGQLFRDIALAVSASVLASLVTAMTLIPTLSARILGGKRHLGEEAASPDG
                     LTGIKRSIESGAGKLPTWIAGVVGHVNRSYARRIAVIAVLTSTAIGGSWLLMPDREYL
                     PAGNSNFVIGIMSPPPGYSPEEIGRIGDGLERELLPRWQVEPGSREARTIEGGGIRDY
                     FFAGFGSLVFIGMRAKDPARSDELVPLLQELLSRVPGTYGVATQWSIFETDATGGSIN
                     LDIRGDDLPALIEVGGALLGRIYERIPGSQVLPQPSLDLANPEIRLVPDRRRMADVGL
                     TNRELGFTVDALVDGAKISDYRIEGREIDLVLVGDDDTFAHGHDLDAIPINTPSGKLV
                     TLGSVARIEPIGGPTQVNHHDRQRAIRLQVTPPRGMPLETAINVIRNDVVGEAERSEL
                     LPPGVVVQLSGTADDLTRTARALSGNFVLAIVVTYLLMAALFQSWIYPLVIMFSVPLA
                     TFGGFVGLRLAHAVTGQNLDVLTMLGFVILIGIVVNNAILIVHQALNLMRGEGRPAED
                     AIPEAVRTRVRPIFMSTATSIFGMLPLVLFPGAGSELYRGLGSVVIGGLALSTVFTLV
                     LVPSLFSLVVEAKSRLAATIERRRHAA"
     gene            7856..>8141
                     /locus_tag="VD788_06420"
     CDS             7856..>8141
                     /locus_tag="VD788_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75940.1"
                     /translation="MGEPVFVRYWLLQLPGQLGLFALMWLLQRWLGFAAWVPFVVVGV
                     WAAKDLALYPLVRGAYATGDRLGAPRKLVGEAGAAEQDLAPSGYVRVRGEL"
BASE COUNT         1130 a         2863 c         2896 g         1252 t
ORIGIN      
        1 actgctgtcc cagctcgtgc tgtacctcgc ggcgcatccc ggcaacgaac ccgcgatggg
       61 aaccgcggta ctgatcgact actacggctt caccgacgag gagaagatcg acgcgctcgc
      121 tccccacttc gattccgcgg acccacgcct gcgggacgcg atctgggagg tgctgctgac
      181 cgtcgatacg ccggcgggcg cacgatcttc cgtcgagctc gtgcgccaag tcgagcaagc
      241 gctcgacggc ggcgcgaagg cggtggcgac gggggacgtg ctcgccacgc ttgcccgcga
      301 cgagtcgtgg tggatccggc tgtacgtggc ccacgtcgcc cgtcgccgac ccgagctggt
      361 tcggccggag gtgctcgctc gcctgcgcga ggacgaggac ccacgtgtcc gccgggccgc
      421 cgacggcggc tcgagcgcgg aggggcgcta accgcccccc ggcgtcgtcc cggtggcggc
      481 gcgcagcagg cggtcgttga ccggagtcgg gatgccgtgc cggcgcccga aggtcacgat
      541 cgcgccgttg cgccacgcca gcgccttggc gccgccccgc atccagccga gttcggtcgt
      601 cgtctcctcg atccggtcga gcgcggctcg cgggtcgacc gacacgccgt agtgcgcggc
      661 ggccgccgtc gcgcactcgg cgacgaggtc gaccagctcg tcgtgccact cgcgcagata
      721 ggtcgcgaga tcgacgtcgt gcgcgaccag cggcagaccg acgacgcagt tccagatgcc
      781 cttgaggatc atctcgcgag cgaaccgatc ccgatcgtcg atgcggacga catcgattcc
      841 tccgcggtcg agcgccgcca cgagcggttc ggcccatgcc ccgcggaaga cggacgggcg
      901 gagcgagcga aagaaggcgc ccttcgaggt gaaccagatc agcccacgcg tcgtttcgcc
      961 gaggtcgtcg tgcaccgcct cgagaaaccc gttctgcacc agcacgacgc gcacgcgcag
     1021 gtcggccggc aggcgggcca gcacgccggc cagcgcttcc tcgcgtacgg cgacgatccg
     1081 cggcgacggg tctcggtggt cggccgccgc gggccagcct cgccggcgcg tgacccgcgc
     1141 cacctgccac ccgtcccgct cgagcgcccc ggcgagtcgg cggcccacct cgccgacgcc
     1201 cacgacgtag actcgtttca tcgacggatt atgccgcacc cacgaccggc accgcggctg
     1261 acggtccgcc gcgtgcgatc ctcggcgctc tgggctacaa ctgagacctg acgaggtgcg
     1321 aatgaggcgt tcgtcatggc ggccggtccg ggcggcgctc gcgctcgtcg tcggtctctc
     1381 ggccccggtg tgtgacgcgg cgacgatcgt gcgcagcgcc gatctcggcg aactcgtcgc
     1441 cgaggccggc tcggcgatac tggccgacgt ggtcgccgtg cgctacggcc gcgacgagcg
     1501 gggtctgcac ggcacctggg tcacggtgcg cgtcgccgac ccgatcttcg gcgacgggct
     1561 gccggcggcc ggcggcgagt acacgttcaa gagctacggc gcgccggtga cgatgccgga
     1621 cggatcgcgg gtctacatcg acggcagccc gtcctaccgg ccgggcgaca gcctgctgtt
     1681 gctgctgctc ccgcggagcc gctggggctt cagcggaacg gccgggctgt cgcagggcgt
     1741 cttccgtgtg cgagtcgacg cgcgcggcga gcgaacggcc gtcggcatgg cgggcaacgc
     1801 cggtgtcttc ggcgagcgtg ggctcgcggg ctggctcgac ccggcggcgc ggtcgggcgt
     1861 gccggccgcg cccgacgcgc cggtgccgta cgccgcgctg cgccgcgcga tcgccgatct
     1921 cgcgcgcacc gccggcaagg tcgcgcgatg agcggcgggc acctgcgatc gctggtcgcg
     1981 acggccggtg tgctgctcgc gctcggctgg aacccgattg aagttcgccc cgacggcccg
     2041 ctgcgctggg agacctcggc tcccgtcgtg tggaaccccg acggcggacc gctcggcatg
     2101 ctcgacaacg ccggtgcgac ggcgatggtg caagacggat tcgccgtttg gcaggcggtc
     2161 ccgacggcga cgatctcctt cgctgtcgga ccgcagatcg tcgacgggag cgacgcgccg
     2221 gtcgacgtca ccgccgacaa cttcctcgag atcgtcaacc tcggaaaccc gcagaacccg
     2281 gtgatcttcg acaacgagca ggagatctgg gccgctctcg gcatctcgac gtcggtgatc
     2341 gggttcgccg gcgcgctcgc ggcctccggc ccgacgatca ccaagggttt cgtcgcgttg
     2401 cagggcgact ggatcgacgg ggtgacggtc aatccgcccg actgcggcgg ccaacgttgc
     2461 gacccggagc tgaaccaagt gtcgcagttc ctcaacatcg tcgtgcacga gatcgggcac
     2521 ttcatcggtt tgggacacgc ggtcgtcaac cgcggcgtcg gcagcctggc cggctgtccg
     2581 gctccgggta tctcggtctt cgcgacgatg ctgccgttcg cgcacttctc gatggagacg
     2641 ctgcaccacg acgacgtcgt cggcgtttcg tcgctctacc cgacggccga gttcgacacc
     2701 gacttcgcgc gcatctcggg gcggctgttc gcgcgcgacg gggcgggccc gctcgacggc
     2761 gccaacgtcg tgctgcggcc cgacacgacc gactgcgcac tgctctacgg cggggcgcag
     2821 gcgacacaat cgggtgtcaa cccggccgag accggcggag ccggcaccta ccgcttcgcc
     2881 ggcctcgacc cggccaccgg ttacacgctg cacgtctccg agatcgacga cggtggcagc
     2941 tacccgatcg gcgggtcgag cccccctccg ctcggcgggc cggccgagtt ccacaacggc
     3001 gtcgacgagg accatttcga cccgcccgac gcgccggacg acttcgagct gctgctcacc
     3061 ggcgaggcgg gaagcgagct gaacggacgc gacctgcggg tgaacaacgc gggcgggccg
     3121 gcggcgatcg cggtctcggg tgccggctcg ttgcagctga ccaagacgct cggcggagac
     3181 ccgttgttga gctggggtcc gccgtgcaac gccgatctcg tgccggatca gacctacgtc
     3241 gtctacgccg ggtcgctgtc gtcggtcggc ggcgtgcagg accatctgcc gctggtctgc
     3301 gataccggcg gggctccttc gctcgaggtg acggccggcg gcggcgatcg gtactacctc
     3361 gtcgcgccgc ggatcgccgg acgcgagggt gcgcttgcca ccggcagcgg cgggctgctc
     3421 cgcacgccgg tcgagacctg tgccgaggtg cgacccgacg agtgtccgtg agcgatcggg
     3481 ccccgtccgg gatcgaccgg ccgtcttgcg ctacgatggc gggaccgtcc gttcctcgcg
     3541 agggcaccgt gtcatgaccc ccgacggccc agcgcgccag ccgcgcccgg cgatgtcgtt
     3601 ccgcttggcg gcatgcgcga cggtctgcgc tgcgacggtc gcctcgggcc aggacggtcc
     3661 accgcccgcg ctcgtcgtca ccgagccggt cgtgcacgag gagctcgtcg agaaaatcga
     3721 gctgatcggc tccgtcgcgc cgatccagga cagcctcgtc gccgccgagg tcgaaggccg
     3781 cgtcgcgctg cgctcgatcg accgcggaga ccaggtcgat cgcgggcagc tgctcgtccg
     3841 cctcgacgcc gcgcgtctcg agcaggagct ggcccgcgtc cgggcgctgc tggtcgacgt
     3901 cgagtcgcag ctcgagctgg cctcgatcca ggagcggcgc gcccgcgacc tgttcgagca
     3961 ggagatcctg tcccagggcg agctcgacca ggcgctcgcg gcgcgccggt cgctcgacgg
     4021 ccgggccgac tcgacgcgcg ccgcgatcgc ctcgatcgag gtcgatctcg accgcagtac
     4081 gatccgcgcg ccgttcgacg gcatcgtcac cgacgtcggc gtcgagatcg gcgaatgggt
     4141 cgatcgcggt gcgtggatcg tccgactgac cgacctcgac acggtcgaga tccgtctcga
     4201 ggttcccgag cagtacttcc gccagctgcg tcgcggggcg tcggcgccgg tcgcggtcga
     4261 cgcctacccc ggcctgacgc tcgacggccg cgtcttcgcc gtcgtgccac aggccgaccc
     4321 cgaggcccgc accttccccg tgctcgtccg cgccggcaac ccggagggga agatcggatc
     4381 gggcatgctg gcccgcgtca tgctgatctt gaccggcggc gagcgcgtgc tgaccgtgcc
     4441 caaggacgcg atcgtgcgcc aggcgcagca agagatgctg tggatcgtcg acggtggcgt
     4501 ggcgcgcccc gtttcggtgc ggaccggccg cggcgccggc acgcgcgtcg aggtgaccgg
     4561 gaacctgagc cccggcgacc tggtcgtggt gcgcggcaac gagcggctga gccccggaca
     4621 accggtcatc gtggaaacgg cccacggaga cggccgtccg tccgagaact agcgcggtgg
     4681 gcatcgtccg gttcgccatc gaccaaccga tcacggtggc ggtcggagcg atcctcgtcg
     4741 cgttgttcgg gctggtctcg ttgaacttcg tcccgatcca gttgacaccc gacatcgatc
     4801 ggccgcgcgc gacggtgctg acgacctggc cgggcgccag cccgttcgag atcgagcgcg
     4861 agatcatcga cgaacaggaa gagcagctca aggggctcga cggcctgctg aagatggaaa
     4921 gcgagtcgca ggacggcttc ggccagatca tgctcgagtt ccccgtcggc accgaccccg
     4981 actcgatcct ggtgcgcgtg tccaaccggc tggagcaggt cccgacctat ccgccggacg
     5041 tcgacaagcc ggtgatcttc accgtcgacc ccggcgcgca ggcgatggcg tggttcatga
     5101 tctacgccga cgacggctac gacatcgatc ccgccaagct gagcgactac gtcgacgact
     5161 tcatcaagcc gccgatcgag cgcgtgccgg gcgtggccca ggtcaatctg ttcggcgggc
     5221 gcgaacgcga gatgcaggtg gtagtcgacc ccaagcggct cgccgctcgc gggctgacga
     5281 tgagcgaggt cgccgccgcg ctcgccgccg aaaacaaaga ctactcggcc ggcgacttcc
     5341 gcgagggcaa acgccggtac gtggtgcgga cggccggcga ctaccggagc ccggaggagg
     5401 tcgagaacgt catcgtcgcg acctcgggcg gcgcaccggt ccacgtccgc gacatcggcc
     5461 gcgtgcaact gggctaccgc aagccgaccg agttcgtccg cgcactcggc cgcccggcgc
     5521 tcgcgttcaa cgcgcaacgc gcgccgggca gcaacgtgct cgacgtgatg gcccgcgtgc
     5581 gggtggtcgt cgagcggctc aacgaggagc tgctgcgccc gcaggggctg cgcctcgaca
     5641 tcgcctacca ggaaaccgac tacatcgaca gcgcgatcgc gctggtgcgc tcgaacatct
     5701 tcgtcggcgg cgcgctggcg ctgatcgtgc tgctgctgtt cttgcgctcg ctgtccggga
     5761 cgttcgtcgt cgggctgtcg atcccgatct cgatggtcgc gacgttcctg gtgatggcgc
     5821 tcgccgggcg ctcgatcaac gtgatctcgc tcgccggcct ggcgttcgcc gtcggcatgg
     5881 tggtcgacaa ctgcatcgtc gtgctggaga acgtctaccg tcaccgtcag atgggcaagc
     5941 gacggatggt cgccgccctc gacggcgccg tcgaggtgtg gggcgcggtg ctcgcctcga
     6001 cgctgacgac ggtcgccgtc ttcctgccgg tgatcttcgt ccgcgacgaa gccggacagc
     6061 tgttccgcga catcgcgctc gcggtttcgg catcggtgct ggccagcctg gtcacggcga
     6121 tgacgttgat cccgacgctg tcggcgcgga tcctcggtgg caagcggcat ctgggtgaag
     6181 aggccgcctc gcccgacggg cttaccggga tcaagcgttc gatcgagagc ggagcgggca
     6241 agctgccgac ctggatcgcc ggcgtggtcg gccacgtcaa ccggagctac gcccgtcgca
     6301 ttgccgtgat cgccgtgctg acgtcgacgg cgatcggcgg gtcgtggctg ttgatgcccg
     6361 accgcgagta cctgccggcc ggcaacagca acttcgtcat cggcatcatg agcccgccgc
     6421 ccggctacag cccggaggag atcggccgga tcggcgacgg cctggagcgg gagctgctgc
     6481 cacgctggca ggtcgagccc gggtcgcgcg aggcgcggac gatcgagggc ggcgggatcc
     6541 gcgactattt cttcgccggc ttcggctcgt tggtcttcat cggcatgcgg gccaaggacc
     6601 cggcgcgcag cgacgagttg gtgccgctgc tgcaggagct gctgtcgaga gtccccggca
     6661 cctacggcgt cgccacgcag tggagcatct tcgagacgga cgccaccggc ggctcgatca
     6721 acctcgacat ccgcggcgac gacctacccg cgctgatcga ggtcggtggc gcgctcttgg
     6781 gtcggatcta cgagcgcatc cccggctcgc aggtgctgcc ccagccgagc ctcgacctgg
     6841 ccaacccgga gatccgcctg gtgcccgacc ggcggcggat ggccgacgtc ggcctgacca
     6901 atcgcgagct gggcttcacc gtcgacgcgt tggtcgacgg tgccaagatc agcgactacc
     6961 gcatcgaagg acgcgagatc gatctggtgc tggtcggcga cgacgacacc ttcgcgcacg
     7021 gccacgacct cgacgcgatc ccgatcaaca cgcccagcgg caagctggtc acactcggtt
     7081 ctgtcgcgcg gatcgagccg atcggcggcc cgacgcaggt caaccaccac gaccgccagc
     7141 gcgcgatccg gctccaggtc acgccgcccc gcgggatgcc gctcgaaacc gcgatcaacg
     7201 tcatccgcaa cgacgtcgtc ggcgaggccg agcggtcgga gctgcttccg cccggcgtcg
     7261 tggtgcagct gtcgggaacc gccgacgacc tgacgcggac ggcgcgggcg ctgtccggca
     7321 atttcgtctt ggccatcgtc gtgacctacc tgctgatggc ggcactgttc cagtcgtgga
     7381 tctacccgct ggtgatcatg ttctcggtgc cgctggcgac gttcggcggt ttcgtcgggc
     7441 tgcgcctggc acacgccgtc accggccaga acctcgacgt gctgacgatg ctcggcttcg
     7501 tcatcctgat cggtatcgtc gtcaacaatg ccatcctgat cgtgcatcag gcgttgaact
     7561 tgatgcgcgg cgagggccgg ccggcggagg acgcgattcc cgaagcggtg cggacccgcg
     7621 tccggccgat cttcatgagc accgcgacca gcatcttcgg catgctgccg ctggtgctgt
     7681 tccccggcgc cgggtccgag ctgtaccgcg ggctgggtag cgtggtgatc ggcggcctgg
     7741 cgctgtcgac ggtgttcacg ctggtgctgg tgccgtcgct gttcagcctg gtcgtcgagg
     7801 cgaagtcgcg cctggcggcc acgatcgaac gccgcaggca cgcggcgtag catcgatggg
     7861 cgaaccggtt ttcgtccgct actggctgct gcagcttccg ggtcagctcg ggctgttcgc
     7921 gctgatgtgg ctgttgcagc gctggctcgg cttcgctgcc tgggtcccgt tcgtcgtcgt
     7981 cggcgtctgg gccgccaagg atctcgcgct ctatccgctg gtgcgcgggg cctacgcgac
     8041 gggggatcgc ctcggagcgc cgcgcaagct cgtcggcgag gcgggcgcgg ccgagcagga
     8101 cctcgccccg tcgggctacg tgcgcgtgcg cggcgagctg t
//
LOCUS       DASPGN010000225         4878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_623992, whole genome shotgun sequence.
ACCESSION   DASPGN010000225 DASPGN010000000
VERSION     DASPGN010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623992"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VD788_06425"
     CDS             <1..568
                     /locus_tag="VD788_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75941.1"
                     /translation="KVAWNGLTPLTAEAFNDPDRVRGHLEQIEPALANLEKVLAITDY
                     QRAADLRAKIDQYVSQMDAQRTTLVQQATADTMTYCKQQWNSFRWWSDRRFEMLSKTP
                     SRADFDEYRVGCRQYIESERIGDKVAELEDEIDQALVLRGCQRTWEQLRQQPDPAALR
                     EFETSCQEYVKLYEVTEQLQQVKNRIGG"
     gene            complement(587..1009)
                     /locus_tag="VD788_06430"
     CDS             complement(587..1009)
                     /locus_tag="VD788_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75942.1"
                     /translation="MRVLFGIGLAVVFATGVALGESPDAGETGGARYCPSAPFSMQVL
                     AIRVDGTHEEVFRGEFASIDELWRGSTWERRDLGLSFARVPNEPGCFTLEREIAEVDS
                     TLTSIRSIYREGSSRITVDYDPSSASSITQVDDGADER"
     gene            complement(1110..1682)
                     /locus_tag="VD788_06435"
     CDS             complement(1110..1682)
                     /locus_tag="VD788_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75943.1"
                     /translation="MIDRQRMRLTHGGHKGAEAEFGRAAMRWDVPETTVSYQGHEMEF
                     AMNVDELSDEDLEKGHVSMEFVFQALGRRFARGKGLRRVIYSMFHVVTRGDELFVVGW
                     IQPDTHVRGGTGWGVELAKFFNRPVHVFDQERDEWMSWDGQRWRPSEPRLPDGPFSAT
                     GTRTLTDAGRRAIADLFERSLGSRESDDAR"
     gene            1849..2961
                     /locus_tag="VD788_06440"
     CDS             1849..2961
                     /locus_tag="VD788_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HXV75944.1"
                     /translation="MRVTPVWKKVCLAIAAPIVFVAVAECGLWLLASAYRRDAPQAET
                     RPSDSAASIRILALGDSFTYGLYVDGSESYPAQLRRRLAGAAVAVDAVGRPGASPTML
                     LDDVDSIVERHRPDVVLLLAGYNINDDDIVRHRERNAADGRPSPATLDRLAARLQRLR
                     TFRLLGQLAIRLRPATDETDPTPRGRMQRFDFRAYQEVNRSALRELAAALLARGVRVV
                     LLNYPQAPVPPNPAGERHEYYYSVYMKRERPLTADDYLEPRRPGEIAINAVIRRVGEE
                     LALPLVDNFRRFESLADKTGYFVRDDEHPNGRGYAILADEVERSLVEHGLLSAPGETA
                     ADQASPAIPASSPGGTAVRSNRITTSSRIRSSGFPG"
     gene            complement(2856..3521)
                     /locus_tag="VD788_06445"
     CDS             complement(2856..3521)
                     /locus_tag="VD788_06445"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXV75945.1"
                     /translation="MMMDHRFGAAATSVALLALLTFGCSNPADDKPEAAVADPEPVPE
                     RSDEAAVFTVDPERSSIHFVGSKITGSHDGGFHQFEGVIELVDGDPTASSVTVEIDTT
                     SLWADDTRLTEHLKSPDFFGVETYPQAVFESTSIEQTADGYTVTGNLDLHGVTKSVSF
                     PAQIEVGPDSVTARAEFFIKRFDFDIVYPGKPDDLIRDEVVIRFDLTAVPPGDDAGMA
                     GDA"
     gene            3749..4591
                     /locus_tag="VD788_06450"
     CDS             3749..4591
                     /locus_tag="VD788_06450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HXV75946.1"
                     /translation="MTESNWPTASSLNHYLADVRRIPRLSREQERRLALEIRAGRHDA
                     INRLVEGNLSFVIKVAGDYRWLGVPLEDLINAGNLGLIEAARRFDAERRVRFTTYAAF
                     WIRKAILEEVAEQSRVVSLPNYQAQLRRRIRKAERALHRGLGRAPNRRELADHLSMRL
                     GHLERVLRRGGQEISLDDPAEDSPGAALRTKLTDAQTEPVDSALHWKIQQRQLRSLLR
                     ELNAQQREVVTLRYGLENASPSTLAEVGARLKVSRERVRQIESAAIRWLRAALDRTRQ
                     FCAR"
     gene            4661..>4878
                     /locus_tag="VD788_06455"
     CDS             4661..>4878
                     /locus_tag="VD788_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75947.1"
                     /translation="MPSQNYVVGAEIEAYCTKCQLDRLHAIETLKSDGNVHRVLCRTC
                     NGIHLFRRPKSDPAGKSHVRTPSKRKAKG"
BASE COUNT          799 a         1740 c         1589 g          750 t
ORIGIN      
        1 gaaagtcgcg tggaacgggc tcactccgct gacggccgag gcgttcaacg accccgaccg
       61 ggtacggggg cacctcgagc agatcgaacc ggcgctcgcc aacctcgaga aggtgttggc
      121 aatcaccgac taccagcgtg cagcggatct gcgggcgaag atcgaccagt acgtctccca
      181 gatggacgca caacgaacga ccctggtcca gcaggccacc gccgacacga tgacctactg
      241 caagcaacag tggaacagct tccgctggtg gtccgatcgc cggttcgaga tgctgtcgaa
      301 gacgccgtcg cgagccgact tcgacgagta ccgcgtgggc tgcaggcagt acatcgagtc
      361 cgaacggatc ggcgacaagg tggcggagct cgaggacgag atcgatcagg cgctcgttct
      421 tcgcggctgc cagcgaacct gggagcagct acgccagcag cccgatcccg ccgcgcttcg
      481 cgagttcgag acctcgtgtc aggaatacgt caagctgtac gaggtcaccg agcagctgca
      541 gcaggtcaag aaccgcatcg gcggctgacc ggcacgaccc ggcggctcac cgttcgtcgg
      601 ccccgtcgtc gacctgggtg atgctgctcg ccgagctcgg gtcgtagtcg acggtgattc
      661 gactcgaacc ctcccggtag atcgatcgga tcgaggtcag cgtcgaatcg acctcggcga
      721 tctcgcgttc cagggtgaag caacccggct cgttcggtac ccgggcgaac gagagaccga
      781 gatcgcgtcg ctcccaagtc gaaccgcgcc agagctcgtc gatcgatgcg aactcgccgc
      841 ggaagacttc ctcgtgtgtg ccgtccaccc ggatcgccaa cacctgcatg ctgaacggag
      901 cgctcgggca gtagcgcgca ccaccggtct cacccgcgtc gggcgattcg ccgagcgcca
      961 cccccgtcgc gaaaaccacc gccaagccga taccgaaaag gacgcgcatt gtcactactc
     1021 ctcgcggccg ccgcgccgca cttccggtcc ccctcagtat cgctcgaatc ggccgtggtc
     1081 gcccgagagg tctccgcgcc gccgcgaagc taacgcgcgt cgtccgattc ccgggaaccg
     1141 agcgaccgct cgaacagatc ggcgatcgcc cgtcgtccgg cgtcggtcag cgtgcgggtc
     1201 ccggtcgccg agaacggccc gtccggcagc ctcggctcgc tcggccgcca ccgctgtccg
     1261 tcccagctca tccactcgtc gcgctcttgg tcgaagacgt gcaccgggcg gttgaagaac
     1321 ttcgccagct cgacacccca accggtcccg ccacggacgt gggtgtcggg ctgaatccag
     1381 ccgacgacga acagctcgtc tccgcgcgtg acgacgtgga acatcgagta gatcacgcga
     1441 cgcagcccct tgccgcgcgc gaatcgccgg ccgagtgcct ggaagacgaa ctccatgctg
     1501 acgtgtccct tttcgaggtc ctcgtcgctc agctcgtcga cgttcatcgc gaattccatc
     1561 tcgtgcccct gataggacac ggtcgtctcc ggcacgtccc agcgcatcgc cgcgcggccg
     1621 aactcggcct ccgcgccctt gtggccgccg tgcgtcagtc gcatccgctg tcggtcgatc
     1681 atcccctggc cctctcccgg tcacccggtc cgtcggttgg ctcgaccccc ggaggttagc
     1741 agcccgtacg gattcggcaa gccaaccggc ggcaccggcc tcggcggggc gcggggagag
     1801 gttgtctcgc cgcgccggtt gcgccacgtt agaggtcgag gagcgattgt gcgagtcacc
     1861 ccggtatgga agaaggtctg cctggcgatc gccgcgccga tcgtcttcgt cgccgtggcc
     1921 gagtgcgggt tgtggctgct cgcgagcgcc taccggcgcg acgcgccgca ggcggagacc
     1981 cggccgtccg actcggcggc ctcgatccgg atcctcgcgc tcggcgactc gttcacctat
     2041 gggctctacg tcgatggctc ggaaagctac ccggcccagt tgcgacgtcg cctggccggc
     2101 gccgccgtcg ccgtcgatgc ggtcgggcgt ccgggggcgt cgccgacgat gctgctcgac
     2161 gacgtcgact cgatcgtcga acggcaccga ccggacgtcg tcttgctgct cgccggttac
     2221 aacatcaacg acgacgacat cgtgcgccac cgcgagcgga acgcggccga cgggcgcccg
     2281 tcgcccgcca cgctcgatcg tctcgccgct cgactgcagc ggctgcggac cttccgcctg
     2341 ttgggtcagc tcgcgatccg cctgcgtccg gcgacagacg agaccgatcc gacgccgcgc
     2401 gggcggatgc agcggttcga cttccgagcc taccaggagg tcaaccgctc ggcgctgcgc
     2461 gagttggccg cggcgcttct cgcccgcggc gtccgtgtcg tgctgctgaa ctacccgcag
     2521 gcgcccgttc cgccgaaccc cgccggcgaa cgtcacgagt actactactc ggtctacatg
     2581 aagcgcgagc gaccgctcac ggcggacgac tatctcgagc cgcgtcgccc cggcgagatc
     2641 gcgatcaacg cggtgatccg ccgcgtcggc gaggagttag cgctcccgct cgtcgacaac
     2701 ttccggcggt tcgagagcct ggcggacaag accggctact tcgtccgcga cgacgagcac
     2761 cccaacgggc ggggctacgc catcctcgcg gacgaagtcg agcggtcgct cgtcgagcac
     2821 ggactgctgt ccgcgccggg cgagaccgcg gcggatcagg cgtcgccggc catcccggcg
     2881 tcgtcgcccg gcggaaccgc ggtcaggtcg aaccgaatca cgacctcgtc gcggatcagg
     2941 tcgtccggct tcccggggta gacgatgtcg aagtcgaagc gcttgatgaa gaactcggct
     3001 cgggccgtga ccgaatcggg acccacctcg atctgggccg ggaacgagac gctcttcgtc
     3061 acgccgtgca ggtcgaggtt gccggtcacg gtgtagccgt cggccgtctg ctcgatcgag
     3121 gtcgactcga agacggcttg cgggtaggtc tcgaccccga agaaatcggg gctcttcaga
     3181 tgctcggtca accgcgtgtc gtccgcccag aggctcgtcg tgtcgatctc gaccgtgacg
     3241 ctcgaggcgg tcgggtcgcc gtcgaccaac tcgatcaccc cttcgaactg gtggaatccc
     3301 ccgtcgtggc tgccggtgat cttcgatccg acgaagtgga tcgacgaccg ttcggggtcg
     3361 accgtgaaca ccgcggcctc gtcgctgcgc tccggcaccg gctcgggatc cgccaccgcc
     3421 gcttccggct tgtcgtcggc cggattcgag caaccgaacg tcagcagtgc cagcagggcg
     3481 accgacgtcg cggcagcccc gaaacgatgg tccatcatca tctccgcttt cctccgcgcc
     3541 ccggaagcgg ggctctgccc ggtcacgcta gcacggtgcc gcggcggtcg cacggtatct
     3601 cgtcgctgcg gtccgttcac cgcagaaacc cgctccggcc gcgaaaccca ccgcgccgtc
     3661 gacgtacact cgggccagcg ggaggttcac cgcaccggat cgaggtttcg accgaccgaa
     3721 tcggtgggcg atacggaggg gggtcgccat gaccgaatcc aactggccca cggcatcctc
     3781 gctcaaccac tacttggcgg acgtgcgccg gatcccgcgt ctcagccgtg agcaggaacg
     3841 ccggctggcg ctcgagatcc gcgcaggtcg tcacgacgcg atcaaccgtc tggtcgaggg
     3901 caacctgagt ttcgtgatca aggtcgccgg cgactaccgc tggctcggcg taccgctcga
     3961 ggacctgatc aacgccggca acctcggcct gatcgaggcg gcgcgtcggt tcgacgccga
     4021 gcgccgcgtc cgcttcacga cctacgccgc gttctggatc cgcaaggcga tcctcgagga
     4081 ggtcgccgaa cagtcccgcg tcgtgtcgct gcccaactac caggcccagc tacgacgccg
     4141 gatccgcaaa gcggagcgtg ccctgcaccg cggtctgggg cgcgccccga accgccggga
     4201 gctggccgac cacctgtcga tgaggctcgg tcacctggag cgcgtgctgc gacgcggcgg
     4261 ccaggagatc agcctcgacg atccggccga agactctccg ggggccgcct tgcggacgaa
     4321 actcaccgac gcgcaaaccg agccggtcga cagcgccctg cactggaaga tccagcagcg
     4381 gcagttgcgc agcctgctcc gcgagctgaa tgcccagcag cgggaggtcg tcacgctgcg
     4441 ttacggtctg gagaatgcct ctcccagcac cctcgccgag gtcggggccc ggctcaaggt
     4501 ctcccgagag cgcgtccgtc agatcgaaag cgcagccatc cgctggctgc gcgccgcgtt
     4561 ggatcggact cgccagttct gcgcgcggtg acccgccggc ctgccccccc acgcgacgcg
     4621 accgacgcat ccgtggtaga ttggcgtcga ggggaatccg atgccaagcc aaaactacgt
     4681 cgtcggggcc gagatcgagg cctactgcac caagtgccag ctcgatcggc tgcacgcgat
     4741 cgagacgctg aagtccgacg gcaacgtcca tcgcgtgctt tgccggacct gcaacgggat
     4801 ccatctgttt cggcgcccca agtccgatcc ggcgggcaag agccacgtgc gcaccccgag
     4861 caagcgaaag gccaaggg
//
LOCUS       DASPGN010000226         7058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1092669, whole genome shotgun sequence.
ACCESSION   DASPGN010000226 DASPGN010000000
VERSION     DASPGN010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092669"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VD788_06460"
     CDS             complement(<1..578)
                     /locus_tag="VD788_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HXV75948.1"
                     /translation="MVRFSHSIFALPFALAGAALAASRCGIEPRQVGWIVVAMVGARN
                     AAMGFNRLVDHRWDAANPRTIGRELPRGVIGRPAVWGLTVSLAAVFVLASFMLNPLCG
                     LLSPVALAVVLGYSYTKRFTWASHLVLGLALAMAPVGGWLAVCGRFSTAAWALGGAVV
                     SWVAGFDVIYACQDVEFDRGSGLFSMPARFGVR"
     gene            complement(611..1933)
                     /locus_tag="VD788_06465"
     CDS             complement(611..1933)
                     /locus_tag="VD788_06465"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV75949.1"
                     /translation="MKIYSAKYVLPISSRAIADGAVAVDRGRIVAVGPAGSVSAAVGG
                     GAERVALGDAVILPGLVNSHTHLELSWTAATRVSCQRGSSHISPALDNAGGVGPPVLA
                     HGDYAGWLRRLLELRDAGTPADAGDHVARALGAMAARGTVAVGDVTNDGSTAVRLAAS
                     PLHAVVFVELYGLRAEETERRLTEAVARLESIAAAPEVAAAGDRLRLALTPHAPHTTG
                     PPLLRALAGRAAAARGALSVHVAESRAEVELLADGEGPLAELFRERAFLPPGWTAPGR
                     SPVEQLRRAGALSARTLAVHCVHLEQVDRSTLQASGATVVTCPRSNAALGVGVAPVPE
                     LLREGVPVALGTDSLASAPDLDLFAEMRAAREIHPDLPAAAVVRMATLNGARALGLSD
                     RLGSIEPGKLAELIVVPLDGAVDRPFDAICGMPEQVWRLEQAPVGTPP"
     gene            2035..2718
                     /locus_tag="VD788_06470"
     CDS             2035..2718
                     /locus_tag="VD788_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75950.1"
                     /translation="MHRIRFAAIWVAAAVIAPAAAWSQDGSKAYRALGLRPQDVLTGT
                     VVRAGVVPGGSEQTVCLATYLTGEEGEADAVNIRLGVFDNSGDELVPVYTRDFGADRG
                     GWVANGDLLVFDVDRDGVKEMIVSYDDFEQPLVDQRVGEVILHDGKTLTTAWTGLLEY
                     DATRAARDVPQERRDRYVREFDWTNTLRTRGATLFVNKKVIAVAGERLPEPRIVAETF
                     ELRGKPDHW"
     gene            2734..3540
                     /locus_tag="VD788_06475"
     CDS             2734..3540
                     /locus_tag="VD788_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXV75951.1"
                     /translation="MFDRVRFVLVQPQNAGNVGSAARALKNLGFSRLELVDPACDPRS
                     DEARRMAVDAGDLLDRIRIHPDLDRALTEATTVVGTTRRLGKHRRPHLALHEASDRLV
                     ALARAGQLAVLFGREDHGLSDRELDRCTDLVYLPSSREYPSFNLAQAVLLVAYQLLLS
                     DGAPPYGAGEAPPADHARREAMLQHLERAFLAIGFLGRDTREVVMRRLRRALGRAALT
                     REEVKLLRGVARQTLWAARKAGLDVPPGDGPEPAGEGEQDGWRERGPADA"
     gene            3506..4645
                     /gene="queG"
                     /locus_tag="VD788_06480"
     CDS             3506..4645
                     /gene="queG"
                     /locus_tag="VD788_06480"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HXV75952.1"
                     /translation="MGGASAARLTRELELRARREGFDRVAVARAERLDRDARALEDWL
                     NRGRHAAMTWMARDPTQRADPTHLLDRCRSVVALAIGYYPGEDEAAPAVGQGRVASYA
                     RGRDYHKVLRPKLRRLTSWLIDETGGRARGFVDTAPVLERGWAERAGLGWIGKNSCLL
                     TRDAGSYLLLAEILTTAELEPTGGPHLDHCGSCTACIDACPTDAIVAPGVVDSNACIS
                     YWTIEHRGSVPEARRAGNADWIFGCDLCQEVCPWNHKSARAVDAARLGRRDDLRALDP
                     LEILELTEERFRARYSGTALMRAKWEGMRRNACIVLGNHGDETAIDGLTRALDDPDPV
                     IRSHAGWALARIAERRPARGSATGDLDAERDESERSVTRPIKPER"
     gene            4922..>7058
                     /locus_tag="VD788_06485"
     CDS             4922..>7058
                     /locus_tag="VD788_06485"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXV75953.1"
                     /translation="MLRPDERPAKQTPTAPVRDAAATSPGSTVDPRRGLRVARRHTKS
                     GVHPYDEIEWELRDAVITNERGEVSFEQRNVEVPGSWSQLATNVVAQKYFRGQLGTPD
                     RENSVRQLIDRVARTAARWGREGGYFATAEDAVVFEAELTYLLVRQMVSFNSPVWFNC
                     GVEERPQVSACFINSVQDTMGSILGLAKTEGMLFKFGSGTGSNLSPLRSSKEHLAGGG
                     TASGPVSFMKGYDAFAGVIKSGGKTRRAAKMVILDASHPDIVEFIECKAEEERKAWAL
                     IDAGYSGEFNVAGGAYDSVFFQNANHSIRVTDEFMGAVLSDSEWTTYAITTGKPVATY
                     SARELMGKIAQAAWICGDPGIQFDTTINTWHTCPNTARINASNPCSEYMFLDDSACNL
                     ASLNLMRFRRADGEFDIESFKHAVEITITAMEIWVDNASYPTTAIEKNSHDYRPLGLG
                     YANLGALLMSRGLPYDSDEGRAYAGAVTALMCGSAYAASAKIAAHNGPFAGFERNREP
                     FLRVMRKHQSHAESFDDKLVPSDLAAAARGVWADVVTYGTEYGFRNAQATVLAPTGTI
                     AFMMDCDTTGIEPDLALVKYKKLVGGGLFKIVNNSVPLALKNLGYGKERIQAIVDYIN
                     ENDTIEGCSELEPEHLSVFDCAFKPANGTRSIDYMGHVKMMAAAQPFLSGAISKTVNM
                     PGDSTIEEIQHAYVEAWRLGLKAIAIYRDG"
BASE COUNT         1138 a         2438 c         2496 g          986 t
ORIGIN      
        1 cgcacgccga accgcgccgg catgctgaac agaccgctgc cgcggtcgaa ttccacgtcc
       61 tggcaggcgt agatcacgtc gaaccccgcg acccacgaca cgaccgcacc gccgagcgcc
      121 caggccgccg tcgagaaccg accgcacacc gcgagccagc ccccgaccgg cgccatcgcc
      181 agcgccagac cgagcaccag gtggctggcc caggtaaagc gcttggtgta ggagtagccg
      241 agcacgacgg cgagggcgac gggggagagc agtccgcaga gcgggttgag catgaacgac
      301 gcgaggacga agaccgcggc gagtgaaacc gtcaagcccc acaccgcggg tcggccgatc
      361 accccgcgcg ggagctctcg cccgatcgtg cgcgggttgg cggcgtccca gcggtggtcg
      421 acgagccggt tgaagcccat cgccgcgttg cgggcgccga ccatcgccac gacgatccag
      481 ccgacctggc gcggttcgat gccgcagcgg gaggcggcga gcgcggcccc ggccagcgcg
      541 aacggcagcg cgaagatgct gtgcgagaac ctgaccatcc gggcccaatc gcgaacggtc
      601 gccccgagct tcacggcggc gtcccgaccg gcgcttgctc gagccgccac acctgctccg
      661 gcatcccgca gatcgcgtcg aacggccgat cgaccgcgcc gtcgagcggc acgacgatca
      721 gctcggcgag cttgccgggc tcgatcgagc cgaggcggtc ggacagcccg agcgcccgcg
      781 cgccgttgag cgtcgccatc cggacgacgg cggccgccgg caagtccgga tggatctctc
      841 gcgcggcgcg catctcggcg aacaggtcga ggtccggggc gctggcgagc gagtccgtcc
      901 cgagcgcgac cggcacaccc tcgcgcagca gctccggaac gggcgcgacg ccgacgccca
      961 gcgcggcgtt gctccgcgga caggtgacga cggtggcgcc cgaggcctgc agcgtcgagc
     1021 gatcgacctg ctcgaggtgg acgcagtgca cggcgagcgt gcgagcggag agcgccccgg
     1081 ctcgccgcaa ttgctcgacc gggctgcgac ccggcgcggt ccagcccggc ggaaggaagg
     1141 cgcgttcgcg aaacagctcg gccagcggac cctcgccgtc ggcgagcagc tcgacctcgg
     1201 cgcgtgattc cgcgacgtgg accgacaacg ccccgcgggc ggccgcggcg cggcccgcga
     1261 gcgcgcgcaa cagcggcgga ccggtggtgt gcggcgcgtg cggggtcagg gccagacgga
     1321 gccgatcgcc ggccgccgcg acctcgggcg ccgccgcgat gctctcgagc cgagctacgg
     1381 cttcagtcag ccgtcgctcc gtctcctccg cgcgcaggcc gtacagctcg acgaagacga
     1441 ccgcgtgcaa cgggctcgcc gccaagcgga ccgccgtcga tccgtcgttg gtcacgtcac
     1501 cgacggcgac cgttccgcgc gccgccatcg cgccgagcgc gcgcgcgacg tgatcgcccg
     1561 cgtcggcggg cgtcccggcg tcgcgcagct cgagcagacg gcgaagccac ccggcgtagt
     1621 cgccgtgcgc gagcacagga ggcccgacgc cgccagcgtt gtcgagggct ggcgagatat
     1681 gtgacgaacc tctctgacag gacacccggg tagcggccgt ccacgacagc tcgagatgcg
     1741 tgtgcgagtt gacgagtccg ggcaggatca cggcgtcgcc gagagcgacg cgctctgcac
     1801 cgccgccgac cgcggcgctc accgacccgg ccggaccgac cgcgacgatc cgcccgcgat
     1861 cgaccgcgac cgccccgtcg gcgatggcgc gcgaggagat cggcaggacg tacttggcgc
     1921 tgtagatctt catggcgacg gttcgggcgg gtccggacgg cgattatagt gcggaagcgg
     1981 gacgactgga attcggcggg gctccgccct aacatcgtcg acggaggtgg ttgcatgcac
     2041 cgtatccgtt tcgcggcgat ctgggtcgcg gcggcggtca tcgcaccggc ggcggcctgg
     2101 tcccaggacg gctcgaaggc gtatcgcgcg ctcggtctgc gtccgcagga cgtgctcacc
     2161 ggaaccgtgg tccgggcggg ggtcgtaccc ggcggatccg aacagacggt ctgcctggcg
     2221 acctacctga ccggcgaaga aggcgaggcc gacgccgtca acatccgcct gggcgtcttc
     2281 gataactcgg gcgacgagct ggtgcccgtc tacacccgcg acttcggcgc cgatcgcggc
     2341 ggttgggtcg ccaacggcga cctcctcgtg ttcgacgtcg atcgcgacgg cgtgaaggag
     2401 atgatcgtct cctacgacga tttcgaacag ccgttggtcg atcagcgggt cggcgaggtg
     2461 atcctgcacg acggaaagac gctgacgacg gcctggaccg gcctgctcga atacgacgcg
     2521 acgcgcgcgg cacgcgatgt cccgcaggag cgacgagatc gttacgtgcg cgagttcgac
     2581 tggaccaaca cgctgcgcac ccgcggcgcg acgctgttcg tcaacaagaa ggtgatcgcc
     2641 gtcgcgggcg agcgcctgcc cgagccccgg atcgtcgccg agaccttcga gctgcgcggc
     2701 aagcccgatc actggtagcc gcccggcccc gccgtgttcg accgagtccg cttcgtcctg
     2761 gtccagccgc aaaacgccgg caacgtcggt tcggctgccc gggcgctgaa gaacctcggc
     2821 ttctcgcgtc tcgagctggt cgatccggcg tgcgacccgc gctcggacga agcccgccgg
     2881 atggcggtcg acgccggcga cctcctcgat cggatccgca tccatcccga cctggaccgt
     2941 gcgttgaccg aggccacgac cgtcgtcggg acgacccgtc gcctgggcaa gcaccgtcgg
     3001 ccgcacctcg cgctgcacga ggcctcggac cggctcgtgg ccctggcgcg tgccggacag
     3061 ctcgccgtcc tgttcggtcg cgaagaccac ggcttgagcg accgcgagct cgaccgctgc
     3121 accgatctgg tctatctgcc gtcgtcgcgg gaatacccct cgttcaacct ggcgcaggcc
     3181 gtgctgctcg tcgcctacca gctcctgctg tcggacggcg caccgccgta cggcgccggc
     3241 gaagccccgc cggccgacca cgcgcggcgc gaggcgatgt tgcagcatct cgagcgcgcc
     3301 ttcctcgcga tcggtttcct cggccgcgac acgcgcgagg tggtgatgcg gcgcttgcgg
     3361 cgagcgctgg ggcgcgccgc gctgacccgc gaggaggtca agctgctccg cggggtggcg
     3421 cgacagacgt tgtgggccgc gcgcaaggcg ggactcgacg tgccgcccgg cgacgggccg
     3481 gagcccgcgg gggaggggga gcaagatggg tggcgcgagc gcggcccggc tgacgcgtga
     3541 gctcgagctc cgggcgcggc gggaggggtt cgaccgcgtc gcggtcgcac gggcggagcg
     3601 tctcgatcgc gacgcgcgcg cgctcgagga ttggctgaat cgcgggcggc acgcggcgat
     3661 gacgtggatg gcgcgagacc cgacgcagcg cgcggacccc acacacctgc tcgaccgctg
     3721 tcgcagcgtc gtcgcgctcg cgatcggcta ctacccgggc gaggacgagg ccgcgccggc
     3781 cgtcggccag gggcgggtcg cgtcgtacgc tcgcggccgt gattaccaca aggtgctccg
     3841 ccccaagctg cggcggctga cctcgtggtt gatcgacgag accggcggtc gcgcgcgcgg
     3901 cttcgtcgac accgcacccg tgctcgaacg cgggtgggcc gagcgcgccg gactcggctg
     3961 gatcggcaag aacagctgcc tgctgacgcg cgacgcgggt tcgtacctgt tgttggccga
     4021 gatcctgacg accgccgagc tcgagccgac cggcgggccg cacctggacc actgcgggag
     4081 ctgcacggcc tgcatcgatg cttgcccgac cgacgcgatc gtcgccccgg gcgtggtcga
     4141 ctcgaatgcg tgcatctcgt actggacgat cgagcaccgc ggttcggttc cggaagcgcg
     4201 gcgcgcgggg aacgcggact ggatcttcgg ctgcgacctg tgccaggagg tctgtccgtg
     4261 gaaccacaag tcggctcgtg cggtcgacgc cgcgcggctc gggcgccgcg acgacctgcg
     4321 cgcgctcgac ccgctcgaga tcctcgagct gaccgaggag cgctttcgag cacgctactc
     4381 gggcacggcg ctgatgcgcg ccaagtggga aggcatgcga cgcaacgcat gcatcgtgct
     4441 cggcaaccac ggcgacgaga ccgcgatcga cggtctcact cgcgccctcg acgaccccga
     4501 tcccgtgatc cgcagccacg cgggctgggc cctggcccgg atcgccgagc gccggccggc
     4561 ccgcgggagc gcgaccggtg acctcgacgc cgagcgagac gaaagcgaac gatcggtgac
     4621 gcggccgata aagccagaga ggtgaccgac ttacggccac cactacatct tgtggagcga
     4681 gatgacgatc agtgctataa gttgtgtatc gaccgtcgat ttcttcttga caccggtcgg
     4741 cgcgcttgtt actgttcgcc gcgcttttcg aaggcggggc gacgacggtc ggcggcagcg
     4801 ccgcgacggt cggaaacgcc ggcaagtggg tagccggcgg cccttgccga agttgtcgcg
     4861 aaccacccct cgcgacccgc ggtgagggga cgacgtcatc attggcagga ggccacaccc
     4921 catgctgcgt cccgacgagc gacccgcgaa gcaaaccccg accgcaccgg ttcgcgatgc
     4981 ggcggccacc tctcccggtt cgacggtcga cccgcggcgg gggcttcgcg tcgcccggcg
     5041 ccacaccaag tccggtgtcc atccctacga cgagatcgag tgggagctgc gggacgcggt
     5101 catcaccaac gagcggggcg aggtcagttt cgagcagcgg aacgtcgagg tgccgggatc
     5161 gtggtcgcag ctcgccacca acgtcgtcgc tcagaagtac ttccgcgggc agctcggtac
     5221 accggatcgg gagaacagcg tccgccagtt gatcgaccgt gtcgcgcgga ccgccgcgcg
     5281 gtgggggcgt gaaggcggct acttcgccac ggccgaagat gcggtcgtgt tcgaggcgga
     5341 gctgacctac ctgctcgtcc gtcaaatggt ctcgttcaac tcgccggtct ggttcaactg
     5401 cggggtcgag gagcgacctc aggtgtcggc ctgtttcatc aactccgtgc aggacacgat
     5461 gggctcgatc ctcggcttgg ccaagaccga ggggatgctg ttcaagttcg gttcggggac
     5521 cggatcgaac ctctccccgc tgcgctcgag caaggagcat ctggccggcg gcggaacggc
     5581 gtccggaccg gtttcgttca tgaagggata cgacgcgttc gccggtgtga tcaagtccgg
     5641 cggcaagacg cgtcgggcgg ccaagatggt catcctggac gccagccacc ccgacatcgt
     5701 cgagttcatc gagtgcaagg cggaggagga gaggaaggcc tgggcgctga tcgacgcggg
     5761 ctattccggc gagttcaacg tcgccggcgg cgcctacgat tcggtcttct tccagaacgc
     5821 caaccactcg attcgcgtga ccgacgagtt catgggcgcc gtactcagcg attcggagtg
     5881 gacgacctac gcgatcacca ccggcaagcc ggtcgcgacc tactccgcgc gcgagctgat
     5941 gggcaagatc gcccaggctg cgtggatctg cggtgatccc gggatccaat tcgacacgac
     6001 gatcaatacc tggcacacct gccccaacac cgcgcggatc aacgcgtcga atccgtgctc
     6061 ggagtacatg tttctcgacg attcggcgtg caatctggcg tcgctgaatc tgatgcgctt
     6121 ccggcgcgcc gacggcgagt tcgacatcga gtcgttcaag cacgcggtcg agatcacgat
     6181 caccgcgatg gagatctggg tcgacaacgc cagctatccg actacggcga tcgagaagaa
     6241 ctcgcacgac taccggccgc tcggtttggg ctacgccaac ctcggcgccc tgctgatgtc
     6301 gcgagggctg ccgtacgact cggacgaagg ccgcgcctac gccggtgccg tcaccgcgct
     6361 gatgtgcggc agcgcctacg ccgcatcggc caagatcgcc gcgcacaacg gcccgttcgc
     6421 cggcttcgag cgcaaccggg agccgttcct gcgcgtgatg cgcaagcacc agtcgcacgc
     6481 cgagtcgttc gacgacaagc tcgtgccatc cgacctggcg gcggccgcgc gcggggtctg
     6541 ggccgacgtc gtgacctacg gcaccgaata cggcttccgc aacgcccagg ccacggtgtt
     6601 ggcgccgacg gggacgatcg cgttcatgat ggactgcgac acgaccggta tcgagcccga
     6661 tctggcgctg gtgaagtaca agaagctcgt cggcggtggg ctgttcaaga tcgtcaacaa
     6721 cagcgtgccg ctggcgctga agaacctcgg ctacggcaag gagcggatcc aggcgatcgt
     6781 cgattacatc aacgagaacg acacgatcga gggttgcagc gagctcgagc ccgagcactt
     6841 gtcggtcttc gactgcgcgt tcaagccggc caacggcacg cggtcgatcg attacatggg
     6901 gcacgtcaag atgatggccg ccgcgcaacc gttcctgtcg ggtgcgatct cgaagaccgt
     6961 caacatgccc ggcgactcga cgatcgagga gatccaacac gcctacgtcg aggcgtggcg
     7021 gctcgggctg aaggcgatcg cgatctaccg cgacggct
//
LOCUS       DASPGN010000227         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_624725, whole genome shotgun sequence.
ACCESSION   DASPGN010000227 DASPGN010000000
VERSION     DASPGN010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624725"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..455
                     /locus_tag="VD788_06490"
     CDS             102..455
                     /locus_tag="VD788_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXV75954.1"
                     /translation="MKLDLRERDGVSLLVLDGQLAAGGELQFREAIDTLLGSGRGRIL
                     VDFTRVSFMDSAGIGELVASHRTVQRLGGVLKILKPSKKIQDSLSLTRLLPIFEVFED
                     EETAIASYASGGNSG"
     gene            623..>3455
                     /locus_tag="VD788_06495"
     CDS             623..>3455
                     /locus_tag="VD788_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXV75955.1"
                     /translation="MNLQVKRLTIALAALGLVVVTALLVIPLFVDVNRYRGVIEARAE
                     QALGREITLGEMGLTLFPRVAVRVDRLTVGGLPGEAGDLLTAERLRVGARLIPLIRQR
                     LEVTQIVVVEPRLALVRGGDGRWNVERLIAGEAGPSGAEQPDESSVPPSFRIDDLRLT
                     GGRLELRDESRPGEPLVATLTDLDLRLSDVRPDGPIGFEMSTRWEQVPQARVRVAGRA
                     GPLFPPGGGSTRVEADAEVEHVDIGRLEPLFAAFLPMPAGLVAGDDVTLSAKLEATTG
                     PGARIALQDVVVDGLVVSLRQGPDGSWNLPRPAGATGGPEGGSASRDSATGFGLSNLE
                     LRDVELAIDGELLPGRRLALTIDDLDARVDRLPTEGPARLTVEAEVAGTGRMTAAGQL
                     GPLARAGGALPLTLDLSFDPVPPVLVGLIEGDGFRLDGGSGRATLALRLTGTFPESLS
                     ARGELELSGYNATLATPDGTTRTIPLSLRADYDATVRGVGETVEIDRLDLDFSGNELS
                     LRGSIAEAGAAHRVDLTLSPTEIAADDLATLAGMLAGKLPIAFESDRPIALEARIEGL
                     VGDGRVPELHGTVELRQFRFMHESLEKPVENVGARITMLGETIRVDDLTGVVGSSDFA
                     GRVSIEGFRAPRIAFELRSNYADFGELFSFLDPGPAETDSDAGRAADEADDPLSAATI
                     AGDIRIERGLFDTLEFDGLQAHMTWADGVLTLDPVDLRLYDGAFRGDVIGDLGGAQPV
                     FEIRGQARQIDIDRFLADNLGSAGMLYGKFSGTVETRTSGADYTSIIRHLTGAGTVEV
                     AEGRVAGLDLLRTLSRVSGLFGEQTISQLGGRLATEGTAFDAMSGGVRFDGGKMRFDG
                     LLLDSPDFRLTGVGDVDLLGAVLDGDFELSMSPELSASMRAEKSRAAQLFWNASESRV
                     ELPFSLEGPFTAPSPRVDWNAVAETAIKGR"
BASE COUNT          495 a         1182 c         1227 g          551 t
ORIGIN      
        1 cctggcgctg gtccggcggt tcgcgctggc gctgacgctg atcgtgatca agaacaagat
       61 catgcgctag cgccggcagg cgctacggta tactcgggcc gatgaagctc gatctgcggg
      121 aacgagacgg cgtcagcctg ctggtgctcg acggccagct cgcggccggc ggcgagctgc
      181 agttccgcga ggcgatcgac acgctgctgg gttccggccg cggccgcatt ctcgtcgact
      241 tcacccgggt ctcgttcatg gacagcgccg gcatcggcga gctcgtcgcg agccaccgca
      301 cggtgcaacg tctcggcggc gtgttgaaga tcctcaagcc cagcaagaag atccaggact
      361 cgctgtcgct gacccgtttg ctgccgatct tcgaagtctt cgaggacgaa gagacggcga
      421 tcgcgagcta cgcgtcaggc ggcaactccg gctgatcgac gccggcgcgc cggagcgtcg
      481 cgcacgccgg cccggccgaa tcccgacgaa aaccggtggt cgagcggggc gtccccggcc
      541 tcgcttgaac ccgcgacggt ttccaataaa gttgagtcgt gtcggtcgag cggccgcgcg
      601 agcggcgagg ctccgagaaa cgatgaacct acaagtcaag cgtctcacga tcgcattggc
      661 cgccctcggg ctggtcgtcg tcaccgcgtt gctcgtcatt ccgctgttcg tcgacgtcaa
      721 ccggtaccgc ggggtgatcg aggctcgagc cgagcaggcg ctcggtcgcg agatcacgct
      781 cggcgagatg gggctgaccc tgttcccccg cgtcgccgtc cgggtcgacc ggctcaccgt
      841 cggcggtctg cccggagaag cgggcgacct gttgaccgcg gagcgtttgc gcgtcggggc
      901 gcggttgatt ccgctgattc gccaacggct cgaggtcacg cagatcgtcg tcgtcgagcc
      961 ccgtctcgca ctcgttcgcg gcggcgacgg acggtggaac gtcgagcggt tgatcgccgg
     1021 tgaagcgggt ccgagcggag cggaacaacc ggacgagtcg tccgtcccgc cgtcctttcg
     1081 catcgacgac ctgcgattga ccggaggcag gctcgagctc cgcgacgagt cacggccggg
     1141 ggaaccgctg gtcgcgacgc tgaccgatct cgacctgcga ttgagcgacg ttcgtcccga
     1201 cgggccgatc ggcttcgaga tgtcgacgcg ctgggagcaa gtgccacagg cgcgggtccg
     1261 cgtcgccggt cgggccggcc cgctctttcc gcccggcggc ggttcgaccc gcgtcgaggc
     1321 cgacgccgag gtcgagcacg tcgacatcgg gcggctcgag ccgctgttcg cggcgtttct
     1381 gccgatgccg gcagggctcg tggccggcga cgacgtcacc ctgtcggcca agctcgaggc
     1441 gaccaccgga cccggcgctc gcatcgcgct tcaggatgtg gtggtcgacg gtctggtggt
     1501 ctcgctccgg cagggtccgg acggctcgtg gaacctgcct cgcccggccg gcgcgaccgg
     1561 cggcccggag ggcgggtcgg cgtcgcgaga ttcggcgacc ggcttcggtc tgtcgaacct
     1621 cgagctccgt gacgtcgagc tggcgatcga cggtgagctg ctgcccggcc gacggctcgc
     1681 gctcacgatc gacgacctcg acgcacgggt cgaccggctg ccgaccgagg gtccggctcg
     1741 gctcacggtc gaggccgagg tcgcgggcac ggggcggatg accgccgccg ggcaattggg
     1801 accgctcgcg cgcgcaggcg gcgcgctccc gctcacgctc gatctgtcgt tcgaccctgt
     1861 cccgccggtc ctcgtcgggc tgatcgaagg cgacggcttc cgcctcgacg gcggctccgg
     1921 ccgggcgacc ctggcgctcc ggctcacggg caccttcccc gagtcgctct cggcgcgcgg
     1981 cgagctcgag ctttcgggct acaacgcgac gctcgcgacg ccggacggga cgacccgcac
     2041 catcccgctc tcgctgcggg cggactacga tgcgaccgtc cggggtgtcg gagaaaccgt
     2101 cgagatcgac cggctcgatc tcgacttctc gggcaacgaa ctgtcgttgc gtggctcgat
     2161 cgcggaggcg ggcgcagccc accgggtcga tctgacgttg tcgcccaccg agatcgcggc
     2221 cgacgacctc gccacgctcg ccggcatgct cgccggcaag ctgccgatcg cgttcgaatc
     2281 ggacaggccg atcgcgctcg aggcccggat cgagggcttg gtcggcgacg ggcgcgtgcc
     2341 cgagctccac gggaccgtcg agctgcggca gttccgcttc atgcacgaat cgctggaaaa
     2401 gccggtcgag aacgtcggtg cgcggatcac catgctcggt gagacgatcc gtgtcgatga
     2461 cctgaccggc gtcgtcggat cgagcgattt cgccggtcgc gtgagtatcg agggctttcg
     2521 cgcgccgagg atcgccttcg agctgcggtc gaactacgcc gacttcggcg agctgttctc
     2581 gtttctcgat ccgggcccgg ccgagaccga ctccgatgcc ggtcgagctg ccgacgaggc
     2641 cgacgacccg ttgtccgccg cgacgatcgc cggcgacatc cgcatcgagc gcggcctgtt
     2701 cgacacgctc gagttcgacg ggcttcaagc gcacatgacc tgggccgacg gcgtgttgac
     2761 gctcgatccc gtcgacctgc gattgtacga cggagcgttc cgcggtgacg tgatcgggga
     2821 cctcggcggc gcgcaaccgg tcttcgagat ccgcgggcag gcgcgccaga tcgacatcga
     2881 ccgtttcctc gcggacaatc tgggcagcgc cggcatgctc tacggcaagt tctcgggaac
     2941 cgtcgagacc cggacgagcg gggccgatta cacatcgatc atccggcacc tgaccggggc
     3001 cggcacggtc gaggtcgccg aagggcgggt cgcgggactg gacctcctcc gcacgttgtc
     3061 acgggtctcc ggtctcttcg gcgaacagac gatcagtcag ctcggcggcc gcctcgccac
     3121 cgaaggcacc gcattcgacg cgatgtcggg cggcgtacgg ttcgacggcg gcaagatgcg
     3181 gttcgacgga ctgctgctcg actcgccgga cttccggctc accggtgtcg gcgacgtcga
     3241 cctgctgggt gccgtgctcg acggcgactt cgagttgagc atgtcgcccg agctgagcgc
     3301 gtcgatgcgc gcggagaaga gccgggccgc gcagttgttc tggaacgcga gcgagtcccg
     3361 cgtcgagcta ccgttctcgc tcgaaggacc gttcaccgcg ccctccccac gcgtcgactg
     3421 gaacgcggtg gccgagaccg cgatcaaggg ccgcg
//
LOCUS       DASPGN010000228        12050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1444094, whole genome shotgun sequence.
ACCESSION   DASPGN010000228 DASPGN010000000
VERSION     DASPGN010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12050
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444094"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VD788_06500"
     CDS             complement(<1..729)
                     /locus_tag="VD788_06500"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXV75956.1"
                     /translation="MILPVGDSPNPPGTAWVTYTVIAINVLVYLLTLPLGARAADPRD
                     PEFVRYLQVVGEERGLGREQLRQLGLQVSEYDLLVFERGFRPAQPSPVDLLTSIFLHA
                     GLMHLAGNMLFLWIYGDNVEHRLGRLGFALLYLATGVVASGGDALLRAGSNLPSVGAS
                     GAISGVLGLYFLWFPHNRVRLWFLLPPFVQIFELPARVVLGFYLVVENLWPLLWSRGD
                     GGISYGAHIGGFVAGLTAAFLISRL"
     gene            890..1312
                     /locus_tag="VD788_06505"
     CDS             890..1312
                     /locus_tag="VD788_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75957.1"
                     /translation="MNRTGAVWAAASIAVALSVTPADADDFQANAVRSFANTMLRGVG
                     AVPTECKPDLLRQVESRQMQIMCARFEGSFARFEIRWDLEMLQRERPDPNSYGTVEPP
                     VTPLTNWEANGVEYDRIYRVEGKAIGVRFNDGDVLMVW"
     gene            complement(1246..1710)
                     /locus_tag="VD788_06510"
     CDS             complement(1246..1710)
                     /locus_tag="VD788_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75958.1"
                     /translation="MFDRSFVRLMPLALGAALLAPMTGAEDDACRAELAVTLDREEVR
                     DDVKLLHFTVTGACDRGCAAVTYDLVIQQVLPNLQWKSVRRTGRLEVRDGRGSDEVVH
                     TMSRDLRLLGQDAVSVSCEPCAGQQPTARPTHQTINTSPSLKRTPIALPSTR"
     gene            1768..2862
                     /locus_tag="VD788_06515"
     CDS             1768..2862
                     /locus_tag="VD788_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75959.1"
                     /translation="MLCRRSIDASVLAGLLCCAASGGPATDERSPSVQVLDERKLESL
                     EQRLSAKGGGGFRFLGALAGDAGLTRSNEIRVYLGRDGATDRLADYRVVSGPFSGALE
                     RLVNRTAQAGGFRVARDGVLRRAKPHPLGNPRTPAAETVALIMERDGSGPTYEYTLLT
                     PGTPRQFTRETEQRFRSGFILRAVRAFDGGFTLAVMERPVVEQRHTPPAEGAPPQYIV
                     IDDRKREQMFDAVGKAAAAGYRVFATVHDGFAGGGSAVIMERATVRGRPYSYFFLGSP
                     SPSELESRLTEGAAAGYRFIAGLAAQGELLMERAPFDEVDVDYLVVTAEAAALIEERL
                     ARAIADGWSWAGLFGEAVVLARDRATSGSR"
     gene            complement(2843..3517)
                     /locus_tag="VD788_06520"
     CDS             complement(2843..3517)
                     /locus_tag="VD788_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXV75960.1"
                     /translation="MKRKPLIVVIGLIAIALADASWAGRPDKDWKRWFGHVAGGYSAP
                     TSTSAEFLEGGYNFNGGATYWPEHWPVGIDLELGYNTFDVKREVLDDIRDATGFDDTL
                     DGDVDIWSLTVDGMWGPPPGGGINVYFAGGLGVYHEEATLSTPVAYSGIVCDPWWYWC
                     VPGVITGEGIIDSESETNFGYNLGVGLTFELGLGSQIYLEAKYHRVERDREPTDYIPI
                     VIGYRW"
     gene            complement(3593..4255)
                     /locus_tag="VD788_06525"
     CDS             complement(3593..4255)
                     /locus_tag="VD788_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HXV75961.1"
                     /translation="MRDRHDRIFAASLAFYVTIVVLTNTVGVKLFTLAGLTLPVSIVF
                     YPLTFLVTDVVSEVYGAARARFFVVMGFLMSVVLLVFSLVGIALPAADVYRLDRAYNE
                     VFGPIWRLLFASMVAFLLAQLVDVQLFHALKRLTSGRHLWLRNNLSTMVSQLVDSVAV
                     NVIFLYGNPAVFSGSLADLLGIVAGVYAVKVLIALADTPLCYLGVWSVKRIIPGLRRE
                     IA"
     gene            complement(4268..5065)
                     /gene="trxA"
                     /locus_tag="VD788_06530"
     CDS             complement(4268..5065)
                     /gene="trxA"
                     /locus_tag="VD788_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXV75962.1"
                     /translation="MGLVDFQTAVVDRSSRVPVVVDFWAPWCGPCRTLGPIIEELARG
                     DAGRWELVKLNTDEQPEVARSYGIQGIPAVKLFVDGRVVAEFVGALPKSEIRRWIDTH
                     LPDPRSARLATLVAEWERRGPELAADLERFVADNPDLPAGRLRLAQAVAATDPTRARQ
                     LIATGAADAPDAELADDVAALIELATVDTAGMPERLARALDDARLALARHAIDETLER
                     LVDAAMLDPRGANQLARRASVALFHMLGQDHDSTRQYQRRLAAALHS"
     gene            complement(5091..6587)
                     /locus_tag="VD788_06535"
     CDS             complement(5091..6587)
                     /locus_tag="VD788_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HXV75963.1"
                     /translation="MAREPSTGGATAGGRSLRSIRAALAWVGPILLVSSVAVAVLLAA
                     RILTYRQRDDPAHLASKRDYLAELRQRESGRADRPNLVVILFDDLGYGDLGAYGGRSI
                     RTPRIDRLAAAGMRFANAYASPYCSASRAALLTGRYAVRSGLDHVLQDPGSLEDQLLK
                     LGRLNRRLPAEEITLAEVLSAAGYATAIVGKWHLGGRSPSLPDDFGFDSFYGLLTSND
                     QGRPVVWADREVAEPHPIDQTTLTRRYTERAVRFIERNADRPFFLYMPHTFPHLPLHV
                     APDRLGRSPAGLYGDVVEELDDSVGAVVDALERTGVAGRTLVVISSDNGPWFQGSPGE
                     LRDRKMSVFEGGTRVPLIAYWPGQLPAAVVIEQSVGLIDLFPTMLELAGLPPPHDRLL
                     DGRSLAPLFRGEPLPDRPVFFHQLAELRAVRSGCFKYHDRHGVYFGNPMDWPWAPMRK
                     RGPWLFDLARDPGESYDVSASQRSVARELERLLETHRQELADNPRGWR"
     gene            complement(6577..7173)
                     /locus_tag="VD788_06540"
     CDS             complement(6577..7173)
                     /locus_tag="VD788_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV75964.1"
                     /translation="MSDPGEVTRLLVAYREGDREAFDRLVPLVYQDLRRIARAHLRRM
                     RAGHTLDTTGLVHEAYLRMVDQTRAEWVDRQHFLSVCARAMRQIVVSNARRHGAAKRG
                     GGDRRVTLDEERIAEARRAEWLVALDRALERLGSHNERLVRVVECRYFAGLSEQETAA
                     ALDSSLRTVQRDWTRARAWLREELGGQTDGMDSGPGGA"
     gene            7272..7814
                     /locus_tag="VD788_06545"
     CDS             7272..7814
                     /locus_tag="VD788_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75965.1"
                     /translation="MAGKAPTLWIVVALIAAGPSWAQTVSWNLVDAGVWQIDPDSADR
                     DEGWFAGGSFELGRKTRFHVLGEYGDFGDFGLWQLGGGWHGLLGDRADLFADLSFSDA
                     DTEDGFKLRFGVRWSVLARLELNGNVGWTDLDFNENESLAVNGIWSFHKRIGVGAGYE
                     AGSEFNLVRAFVRFRFGSQE"
     gene            complement(7816..8655)
                     /locus_tag="VD788_06550"
     CDS             complement(7816..8655)
                     /locus_tag="VD788_06550"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75966.1"
                     /translation="MKHTTVLLIFLVGAVSSVAGDPAELGRDVAAATASLRAGDCERA
                     LGLLADAGRRGSADAQAILGTLYYTGDCTEPDPASAVDWLVAAAEQGSDLAATNLAAM
                     YYRGDGVDRDPARAAGLYRKAADRGYARAQNALAGMLAEGTGVERDPAQAVHWYRLAA
                     EQGLSDAEFHLGHLLFRGEGVERDLQQAAEWFRRAAERGHAMAQYYLGMQYRTGRGVA
                     EDRPTAYAWLVIAARNGHAEAADQQRQVGEALAGADRKRAARLVDEFARYVEPSNDPL
                     GPR"
     gene            8698..9234
                     /locus_tag="VD788_06555"
     CDS             8698..9234
                     /locus_tag="VD788_06555"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HXV75967.1"
                     /translation="MVAEIRANESRRVLSALAAASIAISSAVAGGLTVEHDPEVDFSV
                     FRTYAWSADKGLVAARADVQQAIVQSVDEELRSKGLTEVDPATADLHVVTYALGESWG
                     GTVSGFYRPPDWSIGFITVDSRMVADGTLAIDLYDADEGRLVWHAVARATVDSREQAK
                     RKVSGFVNKAFRKFPPPR"
     gene            complement(9197..9772)
                     /locus_tag="VD788_06560"
     CDS             complement(9197..9772)
                     /locus_tag="VD788_06560"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HXV75968.1"
                     /translation="MFQLRGILAIGSLAGLAAGLGCGGPGQQAEPPSQPAPSSASAGR
                     PAELADTAWVLQSYGPSGEERFPVEGTRPTLSLESAGRAVGMTGCNHFTTTWSADEAD
                     SSFELRAMTMTRMGCPEEVMDQEYALVNAYSSVESYETTGEELTLYYRGGDALLLFFR
                     DTEAGVEAEGEAGEVTGRVPTSGAGTCGTPC"
     gene            9916..11310
                     /locus_tag="VD788_06565"
     CDS             9916..11310
                     /locus_tag="VD788_06565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV75969.1"
                     /translation="MGAGTDREQPSIRESIRILFGASRGYLLVNAANLGDGIAYFGIL
                     TLLTLFMQHDVGFSASWATRSVGWFTGAVTIFMALGGGVVSDRLGVRRALSYCIATLV
                     VGRVLLVVSPELGGSAAVATGAGLSLLLMAVGEGVIQPALYSGIKEYTDRRTETLGYA
                     FLYSIMNVGIAIGGFLSPLIREAWAARVEGRRVLDDPTAGIAGAFWVFVAVTVLMLLV
                     NLTGFTREVERRDRNVIGGASELGPFRWREYLRKLPILDRRFLFFILALLPVRTLFAH
                     QYLTMPDYVTRTMPSEVGARWEWINIINPVVIVIFVPLIAALTLRRRVVDMMIVGTTI
                     SALCSFLLVPEPELTLLLLYMLLWSLGEAVWSSRFLEYVADIAPVHRVGIYMGIAGIP
                     WFLAKTTTGIYAGDVLDAFVPVGGPQRAGTMWLIYGLIALVTPVSLIAARRWLERGAS
                     AAEPEGGSGGRDAA"
     gene            11282..>12050
                     /locus_tag="VD788_06570"
     CDS             11282..>12050
                     /locus_tag="VD788_06570"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXV75970.1"
                     /translation="MGAVVGTPPERAVVQFAIDGELQSVEPLGRGHIHRTWCSRFRGP
                     AGERRFVHQRINRAVFEQPDRVMDNIARVTGHLRRKFREAGSDPTRRTLTIVPARDGS
                     PLLRTIDGEYWRTYRYIDHASTFDAPRDEAHVFQVGQAFATFLRMLADLEGPPLYETI
                     RRFGDTRLRFERFVDVTNRDPLGRAGAVADEIAFVRRRERLAALVVELIAAGAVPRRV
                     AHHDTKINNVLIDDATDEGICVIDLDTVMAGTVLYDFG"
BASE COUNT         1866 a         4162 c         4177 g         1845 t
ORIGIN      
        1 cagccgggag atgaggaacg cggcggtcag gcccgcgacg aagccgccga tgtgcgcgcc
       61 gtaggagatg cccccgtcgc cccggctcca cagcagcggc caaagattct cgacgacgag
      121 gtagaagccg agcacgacgc gtgccggcag ttcgaagatc tggacgaacg gcggcaacag
      181 gaaccacagc cggacccgat tgtgcgggaa ccagaggaag tacaggccga gcacgccgga
      241 gatggcgccc gacgcaccga ccgaggggag gttcgacccg gcgcggagca gggcgtcgcc
      301 cccgctggcg acgacgccgg tcgccagata gagcagcgcg aacccgagtc gcccgagccg
      361 gtgctcgacg ttgtctccgt agatccacag gaacagcatg ttgcccgcca gatgcatcag
      421 cccggcgtgg aggaagatcg aggtcaacag gtcgacgggg gacggctgcg ccggacggaa
      481 gccgcgctcg aatacgagca ggtcgtactc ggagacctgc agcccgagtt gccggagctg
      541 ctctcggccc agaccgcgct cctcgccgac gacctgcagg tagcgcacga actcggggtc
      601 gcgcgggtcg gcggcgcgtg cgccgagtgg cagcgtcaac aggtagacca gcacgttgat
      661 cgcgatcacc gtgtaggtga cccacgcggt acccgggggg ttggggctgt cgccgaccgg
      721 caggatcatc gtcgacccga ctctactcca tccgcccgcc ggccgtgcgg tggaaacaga
      781 cggattcggt tgcgaccggc cggacgcgat cgtatcggtc cggtagggac gctcactcgg
      841 cgactcgtca ctttttcgcg gggtccgata cgcgtccgcg ggacctacga tgaacagaac
      901 cggagcggtg tgggccgccg cgtcgatcgc ggtggcgctg tcggtgacgc cggccgacgc
      961 cgacgatttt caggcgaacg cggtgcgctc gttcgcgaat acgatgctgc gcggtgtcgg
     1021 ggccgtgccg accgagtgca aaccggacct gctgcggcag gtcgagagcc gccagatgca
     1081 gatcatgtgc gcccggttcg aaggcagctt cgcccgcttc gagatccgtt gggacctcga
     1141 gatgctccag cgcgaacgtc ccgatccgaa ctcgtacggt acggtcgagc cgccggtcac
     1201 gccgctgacc aactgggagg ccaacggcgt ggagtacgac cggatctacc gtgtcgaggg
     1261 caaggcgatc ggcgtgcgct tcaacgacgg tgacgtgttg atggtctggt gagtcggacg
     1321 tgcggtcggc tgctgcccgg cgcacggctc gcacgagacc gacaccgcgt cctgccccag
     1381 caagcgcagg tcgcggctca tcgtgtgcac gacctcgtcg ctgcctcggc cgtcgcgcac
     1441 ttccagccga ccggtgcggc gtacgctctt ccactgtagg ttcggcagca cctgctgaat
     1501 gaccaggtcg taggtcaccg cggcacagcc gcgatcacag gcgccggtca cggtgaagtg
     1561 cagcagcttc acgtcgtcgc gcacttcctc gcggtcgagc gtgacggcca gctcggcgcg
     1621 gcaggcatcg tcctcggctc cggtcatcgg cgcgagcaac gccgcgccga gcgcgagcgg
     1681 catcaaacga acgaacgatc gatcgaacat ggcatcctcc tgcggcgaat atagggtcgc
     1741 gggaggcgcg acggtagaat ggtcggtatg ctgtgccggc gatcgatcga tgcgtccgtc
     1801 ttggccggtc tgctgtgctg tgccgcgtcc ggcggaccgg ccaccgacga acggtcgccg
     1861 tcggtgcaag tgctcgacga gcgcaagctc gaatcgctgg agcagcgcct gtcggccaag
     1921 ggcggcggcg gctttcgatt cctcggcgcg ctcgccggcg acgccggcct gacccgctcg
     1981 aacgagattc gcgtctatct cgggcgcgat ggtgcgaccg accggctcgc cgactaccgt
     2041 gtcgtcagcg gcccgttctc gggcgcgctc gagcggctgg tcaaccgcac cgcgcaggcc
     2101 ggcgggttcc gcgtcgcgcg cgacggcgtg ctgaggcggg ccaagcccca tccgctcggc
     2161 aacccgcgca ctccggcagc ggagaccgtc gcgctgatca tggaacgcga cggcagcgga
     2221 ccgacttacg agtacacgct gctcacgccc ggtacgccgc ggcagttcac ccgcgagacg
     2281 gaacaacggt ttcgcagcgg cttcatcctg cgcgcggtgc gcgccttcga cggcggcttc
     2341 acgctggccg tgatggagcg gccggtcgtc gagcagcggc acacgccccc cgccgagggc
     2401 gcgccgccgc agtacatcgt catcgacgac cgaaagcgcg agcagatgtt cgacgcggtc
     2461 ggcaaggcgg cggccgcggg ctaccgcgtg ttcgcgacgg tccacgacgg cttcgccggc
     2521 ggaggctcgg cggtgatcat ggagcgcgcg acggttcgcg gaagacccta ctcgtacttt
     2581 ttcctggggt cgccctcgcc gtcggagctc gagagccgac tgacggaagg cgccgccgcc
     2641 ggctatcgtt tcatcgcggg tctggccgcg cagggcgagt tgctgatgga acgagcgccg
     2701 ttcgacgagg tcgacgtcga ctaccttgtc gtgacggccg aggccgcggc gctgatcgag
     2761 gaacggctcg cgcgcgccat cgccgacggc tggagctggg ccggtttgtt cggcgaggcc
     2821 gtcgtgctgg cacgggatcg ggctaccagc ggtagccgat gacgatcggg atgtagtcgg
     2881 tcggctcgcg atcccgctcg acccggtggt acttggcctc gaggtagatc tgcgagccga
     2941 gaccgagctc gaaggtcagg ccgacgccga ggttgtagcc gaagttggtc tcactctcgg
     3001 aatcgatgat gccctcgccg gtgatcaccc cgggcacgca ccagtaccac cagggatcgc
     3061 agacgatccc cgaatacgcg accggcgtcg acagcgtggc ctcctcgtgg tagacgccga
     3121 gcccaccggc gaaatagacg ttgatgccgc cgccgggcgg agggccccac atcccgtcga
     3181 ccgtcagcga ccagatatcg acgtcgccgt ccagcgtatc gtcgaaaccg gtcgcatcac
     3241 gaatgtcgtc caacacctcc cgtttgacgt cgaaggtgtt gtagcccagc tcgaggtcga
     3301 ttccgaccgg ccagtgctcc ggccagtagg tcgccccgcc gttgaaattg taaccaccct
     3361 cgaggaactc ggcggacgtg ctcgtcggcg cgctgtagcc gcccgcgacg tgaccgaacc
     3421 aacgcttcca gtccttgtcc ggccgcccgg cccagctcgc gtcggcgagc gcaatcgcga
     3481 tcagaccgat caccacgatc aacggcttgc gcttcacgtc ggacttcctc cggctccgtc
     3541 caccgccgct cgattcgctc cgccggccgc tcagtatgcc acaggcgccg gctcaggcga
     3601 tctcgcgccg cagaccggga atgatccgtt tgacgctcca caccccgagg tagcacaagg
     3661 gcgtatcggc cagcgcgatc agcaccttga ctgcgtagac gccggcgacg atgccgagca
     3721 ggtcggcgag cgatccgctg aagaccgcgg ggttcccgta gaggaagatg acgttgacgg
     3781 caacgctgtc gacgagctgc gagaccatcg tcgacaggtt gttgcgcagc cacagatggc
     3841 gacccgacgt caggcgcttc agcgcgtgga acagctggac gtcgacgagc tgtgcgagca
     3901 ggaacgcgac catcgacgcg aacagcagcc gccagatcgg gccgaacacc tcgttgtacg
     3961 cccgatccag ccggtagacg tcggccgcgg gtagcgcgat gccgacgagc gagaagacga
     4021 gcagtacgac gctcatcagg aagcccatca cgacgaagaa ccgcgcgcgc gccgcaccgt
     4081 agacctcgga gaccacgtcg gtcacgagga aggtcaacgg gtagaacacg atgctgaccg
     4141 gcaacgtcaa gccggccaac gtgaacagct tgacccccac cgtgttggtc aacacgacga
     4201 tcgtcacgta gaacgcgagg ctcgcggcga agatcctgtc gtggcggtcg cgcacccggc
     4261 ggattcgtca ggaatgcagt gccgcggcca accggcgctg gtactgccgt gtcgagtcgt
     4321 gatcctgccc gagcatgtgg aacaacgcga cggaggcccg tcgcgcgagc tggttggcgc
     4381 cgcgcgggtc gagcatcgcc gcatcgacca gtcgctcgag ggtctcgtcg atcgcgtgtc
     4441 gcgccagcgc cagccgcgcg tcgtcgagcg cccgcgccag tcgctcgggc attcccgcgg
     4501 tgtcgaccgt ggcgagctcg atcagcgcgg cgacgtcgtc ggccagctcg gcatcggggg
     4561 cgtcggccgc gccggtcgcg atcagctggc gcgctcgcgt cgggtcggtt gccgcgacgg
     4621 cctgggccag acgcagccgc cccgccggca gatcggggtt gtcggcgacg aagcgctcga
     4681 ggtcggcggc cagctccggc ccgcgccgct cccattcggc gacgagcgtc gccagtcggg
     4741 cggagcgcgg gtcggggagg tgggtgtcga tccagcggcg gatctccgac ttcggcagcg
     4801 ccccgacgaa ctcggccacg acgcggccgt cgacaaacag cttgacggcg gggatcccct
     4861 ggatgccgta gctccgcgcg acctccggct gctcgtcggt gttgagcttg accagctccc
     4921 agcgacccgc gtcgccgcgc gcgagctcct cgatgatcgg gcccagcgtg cgacacggcc
     4981 cacaccacgg cgcccagaaa tcgacgacga ccgggacccg cgagctgcga tcgacgaccg
     5041 cggtctggaa atcgacgagg cccatcactc ggcattctac cagccggcga tcagcgccag
     5101 ccgcgagggt tgtcggccag ctcctgcctg tgcgtctcga gcagccgctc cagctcccgc
     5161 gccacggacc gctgactcgc cgacacgtcg taggactcgc cggggtcgcg cgcgaggtcg
     5221 aatagccacg ggccgcgctt gcgcatcggc gcccacggcc agtccatcgg gttgccgaag
     5281 tagacgccgt ggcggtcgtg gtacttgaag cacccggagc ggacggcgcg cagctcggcg
     5341 agttgatgga agaagaccgg ccggtccggc agcggctcgc cgcggaacaa aggcgccagg
     5401 cttcgaccgt cgagcagacg gtcgtgcggc gggggtaacc cggccagctc gagcatcgtc
     5461 gggaacaggt cgatcagccc gaccgattgc tcgatcacga ccgcggcggg cagctgcccc
     5521 ggccaatagg cgatgagcgg cacccgggtg ccgccctcga acacgctcat cttgcggtcg
     5581 cgaagctcgc cgggacttcc ttggaaccac ggtccgttgt cgctcgagat cacgaccagc
     5641 gtccgcccgg cgacacccgt ccgttcgagc gcatcgacca cggcgccgac gctgtcgtcc
     5701 agttcctcga cgacgtcgcc gtagagcccg gcgggagagc gacccagccg gtcgggcgca
     5761 acgtgcagcg gcaggtgcgg gaaggtatgc ggcatgtaga ggaagaacgg tcggtccgcg
     5821 ttccgctcga tgaatcgcac ggcgcgctcg gtgtagcgac gcgtcagtgt cgtctggtcg
     5881 atcggatgcg gctcggccac ctcgcgatcg gcccaaacca ccgggcgacc ttggtcgttg
     5941 ctggtcagca gcccgtagaa cgaatcgaag ccgaagtcgt cgggtagcga cggcgaccgt
     6001 ccgccgaggt gccacttgcc gacgatcgcc gtcgcgtagc ccgcggccga cagcacctcg
     6061 gccagcgtga tctcctcggc cggcaggcgc cggttgagtc gacccagttt gagcagctga
     6121 tcctcgagcg aacccgggtc ctgcaacacg tggtcgaggc cggagcgtac ggcgtagcgg
     6181 ccggtcagca gcgccgcccg cgaggcggag cagtagggag aggcgtaggc gttcgcgaag
     6241 cgcatgcccg ccgcggcgag tcggtcgatc cgcggcgtcc ggatcgatcg cccgccgtag
     6301 gccccgaggt cgccgtatcc caggtcgtcg aacaggatga cgacgaggtt cggccggtcc
     6361 gcccgcccgc tctcccgctg gcgcagctcg gccaggtagt cccgcttgga cgccaggtgc
     6421 gccgggtcgt cccgctgacg gtaggtcagg atcctcgccg ccaacaggac ggcgacggcg
     6481 accgacgaca cgagcaggat cgggccgacc caggcgagcg cggcgcgtat cgaccgaagg
     6541 ctgcgcccgc cggcggtcgc gcctcccgtg ctcggctcac gcgccaccgg gcccgctatc
     6601 catcccgtcg gtctgcccgc ccagctcctc gcgcagccag gcgcgggcgc gcgtccagtc
     6661 tcgttgcacc gtgcgcagcg acgagtcgag cgcggcggcg gtctcctgtt cgctgaggcc
     6721 ggcgaagtaa cggcactcga cgacgcgcac caaccgctcg ttgtggctgc ccagccgctc
     6781 gagcgcccgg tcgagcgcga ccagccattc ggcgcgccgg gcttcggcga tccgttcctc
     6841 gtcgagcgtg acgcgccggt cgccaccgcc gcgtttggcc gccccgtggc gccgcgcatt
     6901 cgatacgacg atctgtcgca tcgcacgggc acagaccgag aggaagtgct gccggtcgac
     6961 ccactcggcc cgcgtctgat cgaccatccg caaataggcc tcgtggacca ggccggtcgt
     7021 gtccagcgtg tggccggctc gcattcggcg caggtgggcc cgggcgatcc tccgcaggtc
     7081 ctggtagacc aacgggacga gacggtcgaa cgcctcgcgg tcgccctccc gataggcgac
     7141 caacagcctg gtcacctcgc ccggatccga cacctgtcgt tcctcctcgg ccgaccgctc
     7201 cgttcgacgg agtcatatta gcccggaagc cggtctatga tgcggccgac gaccgtcgcc
     7261 ggagggaggc catggcgggc aaggcaccca cgttgtggat cgtggtcgcg ctgatcgccg
     7321 ccgggccgag ttgggcccag acggtgagct ggaatctggt cgacgccggc gtctggcaga
     7381 tcgaccccga ttccgccgac cgcgacgagg gctggttcgc cggcggatcg ttcgagctcg
     7441 ggcggaagac gcgcttccac gtcctcggtg agtacggtga tttcggtgat ttcggcttgt
     7501 ggcagctcgg cggcggctgg cacggcctgc tgggagaccg tgccgatctg ttcgccgacc
     7561 tgtcattctc cgacgccgac accgaggacg gcttcaagct ccgtttcggc gtccgctggt
     7621 cggtgctcgc gcggctcgag ttgaacggca acgtcggctg gaccgacctc gacttcaacg
     7681 aaaacgagtc gttggcggtc aacgggatct ggagtttcca caaacggatc ggcgtgggcg
     7741 cgggatacga ggccggaagc gagttcaacc tcgtgcgggc cttcgtgcgc ttccgtttcg
     7801 ggtcgcagga gtgagctacc gcgggccgag cgggtcgttc gacggctcga cgtaccgcgc
     7861 gaactcgtcg acgagccgcg ccgcgcgctt gcggtccgcg ccggcgagcg cttccccgac
     7921 ctggcgctgt tggtcggccg cctcggcgtg cccgttgcgt gcggcgatga cgagccaagc
     7981 gtaggccgtc ggccgatcct cggcgacgcc gcgccccgta cggtactgca tccccaggta
     8041 atactgcgcc atcgcgtgcc cgcgctccgc ggcccgccgg aaccactccg ccgcctgctg
     8101 caggtcgcgt tcgaccccct cgccccggaa cagcagatga cccagatgga actcggcgtc
     8161 ggacaacccc tgctcggcgg ccaggcgata ccagtgcacg gcctgcgccg gatcccgctc
     8221 cacacccgtt ccttccgcga gcatcccggc caacgcgttt tgcgcgcgcg cgtagccgcg
     8281 gtcggcggcc ttgcggtaga gccccgccgc acgagccggg tcgcgatcga caccgtcgcc
     8341 ccggtagtac atcgcggcca aattggtcgc cgcgagatcg cttccctgct cggcggccgc
     8401 gaccagccag tcgacagcgc tcgccggatc gggctcggtg caatcacccg tgtagtacag
     8461 cgtgccgagg atcgcctgcg catcggcgct gccgcggcga ccggcatcgg cgagcaggcc
     8521 cagcgcccgc tcgcagtcac ccgcgcgcag cgacgcggtg gccgccgcga catcccggcc
     8581 gagctcggcc ggatcgccgg caaccgatga gacagcgccg acgagaaaga tcagcaggac
     8641 tgtcgtatgc ttcatccggt tcgctacacc cgacgaccga gccggtcagg agaggtcatg
     8701 gtagccgaaa tccgcgcgaa cgaatcccgg cgggtgcttt ctgcgctggc ggcggcgtcg
     8761 atcgcgatct cgtcggccgt ggccggcggc ttgaccgtcg agcacgatcc cgaggtcgac
     8821 ttctcggtct ttcggaccta cgcctggtcc gcggacaagg gcctcgtcgc ggccagggcc
     8881 gacgtccagc aggcgatcgt ccagtcggtc gacgaggagc tccgatcgaa gggcctgacc
     8941 gaggtcgatc ccgccacggc cgatctgcac gtcgtgacct acgcactggg cgagtcgtgg
     9001 ggcgggacgg tgtcgggttt ctaccgtccg cccgattgga gcatcggctt catcaccgtc
     9061 gactcgcgga tggtggccga cgggacgctg gcgatcgatt tgtacgacgc ggacgagggg
     9121 cggctggtct ggcacgcggt cgcccgggcc accgtcgaca gccgcgagca ggccaagcgc
     9181 aaggtctcgg ggttcgtcaa caaggcgttc cgcaagttcc cgcccccgag gtaggcaccc
     9241 ggcccgtcac ctcgccggct tcgccttcgg cttcgacgcc ggcctcggtg tcgcggaaga
     9301 acaacagcag cgcgtcgccg ccgcggtagt acagcgtcag ctcctcgccc gtcgtctcgt
     9361 acgactcgac cgaggagtag gcgttgacca gcgcgtactc ctggtccatc acctcttccg
     9421 ggcagcccat ccgcgtcatc gtcatcgcgc ggagctcgaa gctcgagtcc gcttcgtcgg
     9481 cagaccaggt ggtggtgaag tgattgcagc cggtcatgcc gaccgcacgc cccgccgact
     9541 cgagcgacag ggtcggtcgc gtgccctcga ccgggaacct ctcttcgccc gaggggccgt
     9601 agctctgcag cacccatgcg gtatcggcca gctcggccgg ccggccggcc gaggcactgc
     9661 tcggggcggg ctgcgacggc ggttcggctt gctgtccggg accgccgcag ccgagaccgg
     9721 cggccagtcc ggccaaagac ccaattgcaa ggattcccct caactggaac atcgccggtg
     9781 ctcctcgtcg acgtcgggcc ggttcgcccg gtacggcgcc gaggatagag aaactgccgc
     9841 gggcgttcaa ggaccggccg ggcgcgtgag ttgtaataag atcggcggcc cgctcctcga
     9901 caggcggagg tcccgttggg cgccggtacc gatcgcgagc aaccgtcgat ccgcgagtcg
     9961 atccggatcc tgttcggcgc gtcccgcggc tacttgctgg tcaacgcggc caacctgggg
    10021 gacggcatcg cctacttcgg gatcctcacg ttgctgacgc tgttcatgca gcacgacgtc
    10081 ggcttttccg cctcctgggc cacgcgcagc gtcggctggt tcaccggagc ggtgacgatc
    10141 ttcatggcgc tcggcggcgg ggtcgtgtcg gatcggctcg gcgtgcggcg cgcgctctcg
    10201 tactgcatcg cgacgctcgt cgtcgggcgc gtgctgctgg tggtctcgcc cgaactgggc
    10261 ggatcggcgg cggtcgcgac cggcgccggg ctttcgctgc tgttgatggc cgtcggcgag
    10321 ggcgtgatcc aaccggcgct atacagcggc atcaaggaat acaccgaccg ccggaccgag
    10381 acgctgggct acgcctttct ctactcgatc atgaacgtcg gaatcgcgat cggcggcttc
    10441 ctgtcgccgc tgatccgcga agcgtgggcc gcgcgggtcg aaggacgccg ggtgctcgac
    10501 gacccgaccg ccggcatcgc cggagcgttt tgggtgttcg tcgcggtcac cgttctgatg
    10561 ctgctggtca acctgaccgg ctttacgcgc gaggtcgagc gacgcgatcg caacgtgatc
    10621 ggcggcgcgt cggagctcgg tccgttccgc tggcgtgaat acctgcgcaa gctgccgatc
    10681 ctcgaccggc gcttcctgtt cttcatcctt gccctgctgc ccgtccgcac gctgttcgcc
    10741 catcagtacc tgacgatgcc ggactacgtg acccgcacga tgccgtccga ggtcggcgcg
    10801 cgctgggagt ggatcaacat catcaacccg gtggtgatcg tgatcttcgt gccgctgatc
    10861 gccgcgctga cgctacggcg ccgggtcgtc gacatgatga tcgtcgggac gacgatcagc
    10921 gcgctgtgca gctttctgct cgtaccggag cccgagctga cgctgctgtt gctctacatg
    10981 ctgctgtggt cgctcggcga ggccgtctgg tcgtcgcgtt tcctcgagta cgtcgccgac
    11041 atcgcccccg tccatcgcgt cgggatctac atgggcatcg ccggtattcc gtggtttctg
    11101 gccaagacca ccaccgggat ctacgcgggc gacgtgctcg acgcattcgt gccggtcggc
    11161 ggcccacagc gagcgggtac gatgtggctg atctacggtt tgatcgccct ggtgacgccg
    11221 gtcagtctga tcgcggcccg ccgctggctg gaacgcgggg cgagtgcggc ggagccggaa
    11281 ggtgggagcg gtggtcggga cgccgcctga acgcgccgtc gtgcagttcg cgatcgacgg
    11341 cgagctgcaa tcggtcgagc cgctcggccg cgggcacatc caccgcacgt ggtgcagtcg
    11401 gtttcgcggt cccgccggcg agagacgctt cgtgcaccag cggatcaacc gcgccgtctt
    11461 cgagcagccg gatcgcgtga tggacaacat cgcgcgcgtc accggccacc tgcgacgcaa
    11521 gttccgcgaa gcgggctcgg acccgacccg ccgaacgctg accatcgtgc cggcgcgcga
    11581 cgggagcccg ctgctgcgta cgatcgacgg tgagtactgg cgaacctacc gctacatcga
    11641 ccacgcctcg accttcgacg caccgcgcga cgaggcccac gtgttccagg tggggcaggc
    11701 gttcgccacc ttcctgcgca tgctcgccga cctggaaggc ccgccgctgt acgagacgat
    11761 tcgccgattc ggcgacacgc ggctccgctt cgagcgcttc gtcgacgtca cgaaccgcga
    11821 cccactcggc cgagccggcg cggtcgccga cgaaatcgcc ttcgttcgac ggcgcgagcg
    11881 gctggcggcg ttggtggtcg agctgatcgc cgccggcgcg gttccgcgac gcgtcgcgca
    11941 ccacgacacc aagatcaaca atgtgttgat cgacgacgcg acggacgaag ggatctgcgt
    12001 catcgacctc gacaccgtga tggcgggtac ggtgctctac gacttcggcg
//
LOCUS       DASPGN010000229        20869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_274663, whole genome shotgun sequence.
ACCESSION   DASPGN010000229 DASPGN010000000
VERSION     DASPGN010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 20869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20869
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274663"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1728)
                     /locus_tag="VD788_06575"
     CDS             complement(<1..1728)
                     /locus_tag="VD788_06575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HXV75971.1"
                     /translation="MKHQSFLVLIVVIGIAAASILAAEEPGYRKIARDLDRGMRGLSA
                     EQTVRVIVTYRPQAGARDLADLAQRDGRRLRHLPSASALAGSLTRAEIAELAADSRVA
                     SISPDRPIRSSMEVTVPTIGADYVVDRVGLTGNGITVALIDSGLTPSRDIPGRRILAS
                     VSFVDGDPSGIDRFGHGTHVAGIIGGAGRGDGVLGIAPDVRFVNLKVMGADGSGYASG
                     VIQAIDWAIANRERYGIRVLNLSLGHPVVEPFVTDPLAIAVERAWAAGLVVVASAGNR
                     GRDGYMTINSPGNDPYILTVGAMNDLDTASRNDDIMTSYSSRGPSRDLVMKPDITAPG
                     NRLISNLAPGSTFGDRYADRVVAPGKIELSGTSGATGVVSGSVAIMLEANPLLSPDQV
                     KVLLMASAEQLATEEVFATGAGYLNLVRAMSFVGPFRTVRFNSSSPMVVRSEDGFELR
                     QVPSLISRLEALYGPNYTALWTDTALWSDTALWSDTALWTDTALWSDTALWTDTALWT
                     DTALWTDTALWSDTALWTDVLAVWTDTALWSDTALWSDTALWSDTALWSDTALWSDTA
                     LWSDTALWSD"
     gene            2073..5054
                     /gene="polA"
                     /locus_tag="VD788_06580"
     CDS             2073..5054
                     /gene="polA"
                     /locus_tag="VD788_06580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HXV75972.1"
                     /translation="MGDCAGKRTLYLVDGTAQLFRAYFAIRGLSNADGLPTNAVFGFT
                     NMLRKMLEEERPDHVAVAFDLGGEVFRHATYADYKANRPPVPEDLDVQAPYAKRACEA
                     LGVSVVELAGYEADDLIATLTRQARQVGFDVVVVASDKDLLQLVGDGVTVLNPSKNLR
                     LDVEGVAREFGVPPERVRDVLGLMGDSVDNIPGVPGVGEKTAIALVSSHGDLERVIAR
                     SRLVVELFQARDALLDLVEAALGERPAEGLARGIDRMRRSLAALSEFEAARESSDNLA
                     GLADDLDRLASDGDTGKTRARRLREIKRDLKALDRGSSKRVWIAIDEHADQARMSKAL
                     ATLDDHAPVDADPSRYRYRGPDRDRARVLFDSLGFKGLGQAFGLEEPSTGRAAGAGEE
                     RGRYDTVLDEAALGEVVEAARAAGRFALRAETDGSDPRRARLLGLSISVEPGSGVYVP
                     LAHVYVGAPEQLGLDTLRRVVGPLLSDATAGKLAHDLKYAGHVLARHGLSVAGWVFDT
                     MVAAFLLNSERSRYRLDALASEYLGLPPDSSAAGTAQDAPASEQIDVERATVRAGRDA
                     DLTLRLGRELERRLDEARLRRLYDTIDGPLLPILQAMETHGIRVDVGLLARMSEEMER
                     SLEVAREKIHRLAGTEFNVDSPKQLREILYDRLGLRPRRKTAKSKVASTDAQTLEELA
                     DDAPIARLILEYRELAKLKGTYVDALPRLVDAESGRVHTCYNPTGAATGRLSSSDPNL
                     QNIPARTEAGRKIRSAFVPEPGWRFLASDYSQVELRVLAHLSEDRELIAAFQAGDDIH
                     RYTAARIAGVPIDLVTDAMRRKAKAVNFGILYGMSETRLAREQGIPRAEARGFIHAYF
                     ERFSAVRAYIDRVREAALREAAVRTLFGRVRYFPQLHQRVNRAVQEQALRAAVNTTIQ
                     GTAADLMKMAMLAVDRRLQADGGPARMLLQVHDELLLEAPADRIDSVARVVRQAMESV
                     HPLNVPLVVDQKQGANWEDAS"
     gene            5130..6281
                     /locus_tag="VD788_06585"
     CDS             5130..6281
                     /locus_tag="VD788_06585"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXV75973.1"
                     /translation="MIEGSVPARKRLLVVDDELSVREILCEGLTEFGFDTATAADAAE
                     ALKILADRPIDLVLTDIEMPGASGMTLLAEVKRRTPDLDVIMVTGVVDTRTAIGAIRE
                     GASDYLTKPFNLDEVQIVVERTLEKRRLIEQNRAYQEHLEDLVEERTEQLRYSYESTL
                     QALVTALDFRDNETQGHSARVVEYAVLVARQMGIGEPELTTIRWGSILHDVGKIGIPD
                     RVLLKPGTLSEEEWVTMKRHPEMGYRMLQHIAFLGPALDIVFSHQERYDGTGYPRGLR
                     AEEIPLGARVFAVVDTFDAMTSDRPYRQGLSIEAARAEIERCSGTQFDPRVAHAFLSI
                     AADRWREIRTRVHEETMAIEERVRRVMGSDSRDDSVQGAAGSGSLPSPS"
     gene            complement(6239..8326)
                     /locus_tag="VD788_06590"
     CDS             complement(6239..8326)
                     /locus_tag="VD788_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXV75974.1"
                     /translation="MVRALSTILLCLLPVAASGCGAAGRTPASSAPASSEELLTVRAQ
                     VLRAEDRRLVDDQLLAALRHAQPEIRAAAVRALGRIGDPGQSSRLEEATQDDAPEVRA
                     AAAFAIGRIGAAGALERLEPLGQDESATVRAATADALGLIATTDAEPAVTRLLADPDP
                     AVAARACLAVPRFARPAFAVRSLIELGRSDRDELIFACNWALSMLSAEPERIGREDRV
                     AARDRMIELTASRYTVLRRMAARGLVRPTREEEAASVGALVDDRDSSVRIEAIRAISF
                     PGAPLEPFLSKILEKGQEEDARVYLEFVDGLGRMRGPDIVQTLADIVVFDPRVWIKER
                     AVVALGQATNYSVKMANGLSRSDHAAVRRATADLLIGRIDDETIEFARRLRDDQDPSV
                     RAAIIPVFAEVDGLLTENLATSVESADPRIRRALALAAGRVLADEQAASAEIDDAFAL
                     LERLWRDAGESSDVALGVAVLRATGRARPDDRARALLEAGLESAAWRPRVVAAQLLFS
                     AYGDDRRQSLGPATDHPVEHYVALLRWAAKPRAAIVTVERRGFEPGRFSIALDTANAP
                     LASREFARLAESGAFDGLPISRLVADVALFAGTPAAEAVPDRGFDLREEIGNDIVLAG
                     TLAWGADGLDPLDGNWFISLRPRPELLGRVDGFARVVRNLAGVVALMLPSDLVKSVVI
                     YEGDGSEPLPAAP"
     gene            8513..9229
                     /locus_tag="VD788_06595"
     CDS             8513..9229
                     /locus_tag="VD788_06595"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HXV75975.1"
                     /translation="MSNGRHQVSRKLSLLVLGSLVATAAAADDDLDRVLARFDDVQGS
                     IQSLSADFTLTTTNQLLLDPIAAQGRFYMTKPDSIRWEYSQPEEMRFVIFGDQYTGFF
                     PEQKRAERKNIQRWREHIFRFFGFGQGTSELRKFYDISLDPDHDPSNGTYLLILEPSK
                     KRVRKRIPEVRFWVDRTSYLPAKVEYVNQNGNTSLLELRHIQVNPDLAASTFQVEVPR
                     DFKVTKGFSGLPDFDTSSGE"
     gene            9226..10188
                     /locus_tag="VD788_06600"
     CDS             9226..10188
                     /locus_tag="VD788_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HXV75976.1"
                     /translation="MTSRTDEPVGLILDPVFERHETGPGHPERSARLVAIREAIERDA
                     LDCRAERVALESADDRLLELVHDAAHLRRVHEACESGEPTLDSMDTAICADSDRVARL
                     AAGSLVALCRRVARGELSRGFAAIRPPGHHAERDLAMGFCLFNNVAIAARYLREHEGV
                     ARVLIVDWDVHHGNGTQHLFEQDPSIFFFSLHQWPLYPGTGAAEERGVGAGLGTTLNC
                     PLAPGAGDAEFLFALREVMLPAALDFRPEFVLVSAGFDAHYADPLANLEVSTEAYGEA
                     TDIVCDLADRVAAGRVVSTLEGGYDLRALGESVALHLRRLQRLA"
     gene            complement(10193..12037)
                     /locus_tag="VD788_06605"
     CDS             complement(10193..12037)
                     /locus_tag="VD788_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HXV75977.1"
                     /translation="MRPYRLQRSIWLPVAFASVTLLSSAGCAPADHDPPDYPATAEGA
                     ARFVGDAEARLLDRWIAAERASWVQENFITVDTERIAADAAQERIATTVELALASTRF
                     DGVALPDELSRKIRMLKTSLPLVGPPDAALQAELAAIAASLSSIYGKGRYCPDGDGAD
                     CLDLGQLSRTLASSRDPARLLEAWSGWRTISPPMRDKYVRFVSLANAGATALGFDDLG
                     ALWRSGYDMPPERFADEVERLWGQVRPLYDALHCHVRARLSDYYGEQVVPPGQPIPAH
                     LLGNMWAQDWSNVYDLVGPAGGDAGVDLTELLVAAEVDPVGMVRIAERFFTSLGFEPL
                     PASFWERSLFTQPAERDVVCHASAWNIDWVDDLRIKMCIDVTAEDFITVHHELGHNFY
                     QRAYGGLDPLYRNSANDGFHEGIGDTIALSITPEYLAQLGLLDREPGSAGDLGLLMRT
                     ALDKIAFLPFGYLVDQWRWKVFSGEIPAERYNATWWELRERYQGIASPVPRSEADFDP
                     GAKYHVPANTPYTRYFLAYILEFQFHRALCAISGHDGPLHRCSIYADGEAGRRLIEML
                     RMGASRPWPDALEALTGQREMDATAILDYFAPLIAWLDEQNRERSCGW"
     gene            12161..13069
                     /locus_tag="VD788_06610"
     CDS             12161..13069
                     /locus_tag="VD788_06610"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXV75978.1"
                     /translation="MGDGKTRYDDYLAEELKNFEELAKFLRPSPGGIPRLDGIDIGGL
                     FMPLYGVIGGDLVTYIDFSSRYDLPRRVERARSAGLTAVAEKLSLLHERAGILVADIS
                     GHRITDALIAAMLHQAFLLGAYYELDMFGEITTRLFEQLNQRFYRTTTINKYLTMIYG
                     EISRQGTFRFLSAAHQPPAVFSREYGQFVTISPDRLVTLPPVAMFPSRADPDASNHPN
                     LIGYKERYEVNEINLLAKGDILLLSTDGFAEHADGSFFPEKVRQLLAASDGSSASQIC
                     DRLRQAIIAAGEQTDDLSVVVIKKTG"
     gene            complement(13053..14156)
                     /locus_tag="VD788_06615"
     CDS             complement(13053..14156)
                     /locus_tag="VD788_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HXV75979.1"
                     /translation="MDRGLRDRLQASGAVFDALSGEALHFGRPAAELDAALDRCALVD
                     RGMLGRLLASGPDLLSLLNRLSTAKLDSLAVGQGVTTVVTTAKGRIVERLFVHRLADR
                     SVLLVSGAGAAPRVVAYLDRFTFAEQTGLDDVSAKTGQLSVVGPRAAEALAAAGLPRP
                     LPAASVVAELGGVEMHVLGQDGLSTAGYSVFLPAGVAGDAWRLLREATERCGGLPAGT
                     SAAEAWRVLHGLPAPGHELTEDYNPLEAGLYDAVSFAKGCYVGQEVVARLNTYDKVSR
                     SLVGLVFDRSIELPAAGTPLYHDGREAGVLTSAVTPPGRNRSIAIGYLKRRAAGAGAE
                     LFVGASESALRARVVDLPFPDAAGRRNGASRSS"
     gene            complement(14165..15013)
                     /locus_tag="VD788_06620"
     CDS             complement(14165..15013)
                     /locus_tag="VD788_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HXV75980.1"
                     /translation="MKQTETRLVRIGHSPDPDDAFMFHGIATGAVDTGPFRVEQVLEE
                     IESLNRRALRGELEVSAVSIHAYAHLAERYALMHCGASMGDGYGPCVVARRAFDPGEL
                     AHRTIAIPGTLTSAYLALRLLVGGPLRYDVLPFDRIPQAVLDGRVDAGLIIHEGQITY
                     PREGLRLVVDLGRWWGERTGGLPLPLGGNVVRRDLGRETMVALTRMLEQSIDHGLRHR
                     DPALAYALDFGRGLDRELTDRFVGMYVNDLTRDYGERGREAIRRFLAEGCAAGVVPPV
                     DTIEFV"
     gene            15148..15594
                     /locus_tag="VD788_06625"
     CDS             15148..15594
                     /locus_tag="VD788_06625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV75981.1"
                     /translation="MSRRSRRLTGIAALVVVAAGALAAAPDDEPDVVVVQPVLIAFKG
                     SLPAKQVDRSKKEARALAEELFEQAQAEDADFDAMVKQYTSDSYPGVYKLTNHDAPIT
                     GGAYQRKEMATRFGDVAFKLEVGEVGLAAYSVTASPYGWHIIKRLE"
     gene            15597..16769
                     /locus_tag="VD788_06630"
     CDS             15597..16769
                     /locus_tag="VD788_06630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXV75982.1"
                     /translation="MADDRLRTEADRIAPELVVWRRRLHQQPELGFEEHETSRFVAER
                     LAQLGVEVRTGLAGTGVAGILRSPRRDRPGVLLRADMDALPVQEVDGREYGSRVPGRM
                     HACGHDGHTAMLLGAAKLLEARKSELRRDVVFCFQPAEEGLGGAERMIDDGVLELVET
                     GSVFGLHLWSGAEVGTAMMRAGPAMAAQDEIEVRILGRGGHGALPHTARDPIVAASLA
                     VVALQGIVARNVDPVETAVVTVGSLHAGTAPNVIPAEARLAGTLRSFSEEVRELLRLR
                     SREVIESSAIAAGCTADYLLKKGFPAVVNDAASVEIARRLAVDVFGEGNVVEHPPIAA
                     AEDFSYFLGRRPGAFIFVGAANTARGIDAPHHSPMFDIDEAALPRGAELLARLALD"
     gene            16809..16985
                     /locus_tag="VD788_06635"
     CDS             16809..16985
                     /locus_tag="VD788_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75983.1"
                     /translation="MAKTKTEFDKPQVKAAIKELKQRRQRALEAKDREELKKVRRRIH
                     RLKHELRASVRTRS"
     gene            complement(17049..18014)
                     /locus_tag="VD788_06640"
     CDS             complement(17049..18014)
                     /locus_tag="VD788_06640"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV75984.1"
                     /translation="MSASSAPATRRLRRGRGSPTAPPPRPLVAWVIAHLIRLFAMLPL
                     WTIQAAGATIGRIAGLLPIPERTVAEINVGIAFPELAPAERRRLARRSMIETAKTFAE
                     FSAAWCWSCERIGRHVRTVHGDAAIRRALANGRGVILAAPHLGSWEIGGIEVSKRFPM
                     TTLYRRPRLAELEPLVKSGRERMGAELVPSGLTAVRTLLAALRRGELVAILPDQEPAE
                     GMGIFVPFFGVQANTMTLLSKLAARTGALVVMGYAERLPRGRGFDLHFITTSDEIGDA
                     DLERSAAALNREVERCVRRCPEQYIWSYKRFRIRPPGGAKNPYAR"
     gene            complement(18061..19533)
                     /gene="rfaE1"
                     /locus_tag="VD788_06645"
     CDS             complement(18061..19533)
                     /gene="rfaE1"
                     /locus_tag="VD788_06645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HXV75985.1"
                     /translation="MTELNALRHRIHDFGRARVLCLGDVMLDEFVYGDAVRLSPEAPI
                     PVVNVRRRLLMPGGAGNLTRNVTTLGALGTVIGVVGRDEMAAVLRRLLEEQDRLRSHL
                     VPCSDRLTTVKTRFVVHNQQMLRADVEHTGPIEGELAERLLSIYEAELEHADLVVLSD
                     YAKGVLSDAVLRPAIDLARSRRKTVIADPKTRDFSRYRGVYLLMPNAGELRTATAMPC
                     DTDDEAVAAARSVVEQAGVDNLLVTRSERGMTLVTSGGEPVQLPARAREVHDVSGAGD
                     TVAATMAVALASGWELADAARLANVAAGIVVGKVGTATVYPRDLVAEIHRADIDSVNG
                     EIVTLKQALELVDRWRAQGLRVGFTNGCFDLIHPGHVSLLNQAKAACDRLIVGLNSDS
                     SARRLKGPSRPIQSQTARSVVLASLAPVDLVVVFDEDTPLRLIERIKPDVLVKGADYT
                     KDRVVGADLVTSYGGRVLLAKLEEGHSTTGTIARVGGGSS"
     gene            complement(19530..20120)
                     /locus_tag="VD788_06650"
     CDS             complement(19530..20120)
                     /locus_tag="VD788_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HXV75986.1"
                     /translation="MDVADYFERELRQHLDIVRLVGDELGERLATATRICADALDHGN
                     KLLFFGNGGSAADAQHFAAELVVRYRADRPALAAIALTTDSSALTACANDLGYEQVFA
                     RQIEALGRPGDVAIGSSTSGRSPNVALAFRTARRLGLETVALTGGDGGELVPLVDVAL
                     VVPSDVTARIQEVHLLLGHTLCGALEARAILERERR"
     gene            20316..>20869
                     /locus_tag="VD788_06655"
     CDS             20316..>20869
                     /locus_tag="VD788_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75987.1"
                     /translation="MNVAVGRVDGTAAQRPPPSVRAIVVAIWVALGSAAASPDPPCLN
                     DGPHPGRTEQWIAAAIDVLSAAGHPPRRYRLELRMEPAARPDFPELGVERVRSVVFVP
                     RDPTTDYPLRVHPSRPCSLAWVWQPGSFTDWQRAVIDRAQAISAARAAGEVQQVQVIE
                     TARSIEILVVTGDGSSERIVFEKS"
BASE COUNT         3301 a         7241 c         7186 g         3141 t
ORIGIN      
        1 gtcgctccac agcgcggtgt cgctccacag ggcggtgtcg ctccacaggg cggtgtcgct
       61 ccacagggcg gtgtcgctcc acagggcggt gtcgctccac agggcggtat cactccacag
      121 ggcggtgtcg gtccagacag cgagcacgtc cgtccagagc gccgtatcac tccagagcgc
      181 ggtatcagtc cacagggccg tgtcggtcca gagcgcggtg tcagtccaca gggccgtgtc
      241 actccacaac gccgtgtcgg tccagagcgc ggtgtcgctc cacagggcgg tgtcgctcca
      301 caacgccgtg tcggtccaca gcgcggtgta gttcgggccg tacagcgcct cgaggcgcga
      361 gatcaacgac ggcacctgac gcagctcgaa tccgtcttcg ctgcggacga ccatcggcga
      421 ggaggaattg aagcgcaccg tgcggaacgg accgacgaac gacatcgcac ggaccaggtt
      481 gaggtaaccc gcacccgtag cgaagacctc ctcggtcgcg agctgctcgg cggaggccat
      541 cagcagcacc ttgacctggt ccgggctcag cagcgggttg gcctcgagca tgatcgcgac
      601 gctgccggag acgacaccgg tggcaccgct ggtgcccgac aactcgatct tgccgggggc
      661 gacgacgcgg tcggcgtacc ggtcgccgaa ggtcgaccct ggtgccaggt tcgagatcag
      721 acggttgccc ggtgccgtga tgtcgggctt catcaccaga tcgcgcgaag gaccgcgact
      781 gctgtagctc gtcatgatgt cgtcgttgcg cgaagcggtg tcgaggtcgt tcatcgcgcc
      841 gaccgtgagg atgtaaggat cgttgccggg cgagttgatc gtcatgtaac cgtcccgtcc
      901 gcggttgccg gccgaggcga cgacgacgag ccccgcggcc cacgcgcgtt ccaccgcgat
      961 cgccaacgga tcggtgacga acggctcgac gaccgggtgg ccgagcgaga ggttcagcac
     1021 gcgaatgccg taccgctccc ggttggcgat cgcccagtcg atcgcctgga ttacgccact
     1081 ggcgtagccc gagccatcgg cccccatcac cttcaggttg acgaagcgga cgtcgggcgc
     1141 gatgcccagc acgccgtcgc ctcggcccgc cccgccgatg atccctgcca cgtgggtgcc
     1201 gtggccgaag cggtcgatcc cgctcgggtc cccgtcgacg aaactgacgc tggccaggat
     1261 gcgtcgtccg ggaatgtcgc gcgacggcgt cagccccgaa tcgatcagcg ccaccgtgat
     1321 tccgttgccg gtgagtccga cgcggtcgac gacgtagtcc gcgccgatcg tcggaaccgt
     1381 cacctccatc gagctgcgga tcggccgatc gggggaaatc gacgcgaccc gcgaatccgc
     1441 cgccagctcc gcgatctcgg cccgggtcaa cgagccggcc aaggcgctcg cgctcggcag
     1501 gtggcgcagc cggcggccat cccgctgcgc gaggtcggcg aggtcgcgcg cgccggcctg
     1561 cggacggtag gttacgatca cgcgcacggt ctgctcggcc gagaggcccc gcatacctcg
     1621 atcgaggtcg cgcgcgatct tccggtagcc gggttcttcg gcggccagga tggacgcggc
     1681 cgcgatgccg atgacgacga tcagcacgag aaaagattga tgtttcatgg ttgccccctg
     1741 tgtggtccag ttccccatcg cgacgggacg tcactgcaag ccgcggaccg gatcgtgcca
     1801 gatcgtacaa ggtgctgtac tcaaatgact tacggccgat accgggcact tgtgctccct
     1861 cgggagatcg tctcaaactg aggcggttcg gcgacttgat cctgtctaac agtttgaaag
     1921 taaaggagtt atcagaatgc gtgtcggcgg tggccgaacc atctcaatct gagacggttc
     1981 ggcgggttcc cggcccgcga gcggaggcgg attaggtatc atgcgcgagg ccgcgacggt
     2041 cacggtgatg cacgggcgat ggcgagggcg atgtgggcga ttgcgccggg aagcgtacgc
     2101 tctatttggt cgacgggacg gcccagctgt ttcgcgccta cttcgccatc cggggtctgt
     2161 cgaacgcgga cgggctgccg acgaatgccg tcttcggctt caccaacatg ctgcggaaga
     2221 tgctcgagga ggagcgaccg gaccacgtcg ccgtcgcctt cgacctcgga ggcgaggtct
     2281 tccgccacgc cacctacgcc gactacaagg cgaaccgacc gcccgtgccc gaggacctcg
     2341 acgtccaggc accctacgcc aagcgcgctt gcgaggcgct cggcgtctcg gtcgtcgagc
     2401 tcgcgggcta cgaggccgac gacctgatcg cgacgctgac caggcaggca cgccaggtcg
     2461 gcttcgacgt cgtcgtcgtc gcgtcggaca aagatctgct ccaactcgtc ggcgacggtg
     2521 tgaccgtgct caacccgtcg aagaacctgc gtctcgatgt ggaaggagtg gctcgggagt
     2581 tcggcgtacc cccggagcgc gtgcgcgacg tgctgggtct gatgggggac agcgtcgaca
     2641 acatcccggg cgtgcccggt gtcggcgaga agaccgccat cgcgctcgtc tcgagccatg
     2701 gtgacctcga acgcgtcatc gctcggtccc gtctcgtcgt cgagctgttc caggcgcggg
     2761 acgcgttgct cgacctcgtg gaggcggcgc tcggcgagcg cccggccgaa gggcttgccc
     2821 gcgggatcga ccgtatgcgc cggtcgctgg cggcgctcag cgagttcgaa gcggcccgcg
     2881 agtcgtccga caacctcgcc gggctcgccg acgacctcga tcggttggcc agcgacggcg
     2941 acaccggcaa gacgcgggcc aggcggctgc gcgagatcaa gcgcgatctc aaggcgctcg
     3001 accgcggcag ctcgaagcgg gtgtggatcg cgatcgacga gcatgccgac caggcgcgga
     3061 tgagcaaggc cctggcgaca ctcgacgacc atgcaccggt cgacgcggat ccctcccgct
     3121 atcgctatcg cgggcccgac cgtgatcggg cgcgcgtgct gttcgactcg ctcggtttca
     3181 agggtctcgg ccaggcgttt ggcctcgagg agccgagcac cggccgggcg gcgggcgccg
     3241 gcgaggagcg agggcgctac gacaccgtgc tcgacgaggc ggcgctcggc gaggtggtcg
     3301 aggccgcccg cgccgccggg cgattcgcgc tgcgggcgga gaccgacggg agcgacccgc
     3361 ggcgcgcgcg cctgctcggc ctgtcgatct cggtcgagcc cggcagcggc gtctacgtcc
     3421 cgctcgcgca cgtctatgtc ggggcacccg agcaactcgg gctcgacacg ctgcgccgcg
     3481 tcgtcggccc gttgctttcc gacgcgaccg ccggcaagct ggcccacgac ctcaagtacg
     3541 ccggtcacgt gctggcacgc cacgggctgt cggtcgccgg ctgggtgttc gatacgatgg
     3601 tcgccgcctt cctgttgaac tcggagcggt cgcgctaccg tctcgacgcc ctggcctcgg
     3661 agtacctggg tctcccgccg gattcgtccg ccgccgggac ggcccaggac gcgcccgcct
     3721 cggagcagat cgacgtcgag cgcgccaccg tccgggccgg ccgcgacgcc gacctcacgc
     3781 tgcgcctggg gcgcgagctc gagcgtcggc tcgacgaggc ccggctgcgc cggctgtacg
     3841 acacgatcga cggaccgctg ctgccgatcc tgcaggcgat ggagacgcac ggcatccgtg
     3901 tcgacgtcgg gttgctcgcg cgcatgtccg aggagatgga gcgctcgctc gaggtggcgc
     3961 gggagaagat ccaccggctg gccggcaccg agttcaacgt cgactcgccc aagcagctgc
     4021 gcgagatcct ctacgaccga ctggggttgc ggccgcgccg caagacggcc aagagcaagg
     4081 tggcctcgac cgacgcccag acgctcgagg agctcgccga cgacgcgccg atcgcgcggc
     4141 tgattctcga gtaccgcgag ctggccaagc tgaagggaac ctacgtcgat gcgctgccgc
     4201 ggctggtcga tgccgagagc ggacgggtcc acacctgcta caacccgacc ggcgcggcga
     4261 ccggtcggtt gtcctcgtcc gacccgaatc tgcagaacat ccccgcgcgc acggaggccg
     4321 gtcgcaagat ccgctcggct ttcgtaccgg agccgggttg gcggttcctc gcgtcggact
     4381 actcgcaggt cgagctccgc gtgctggctc acctgagcga agatcgcgag ctgatcgcgg
     4441 cgttccaggc cggcgacgac atccatcgct acaccgcggc gcggatcgcc ggcgtgccga
     4501 tcgacctcgt caccgatgcg atgcgcagga aggccaaggc tgtcaatttc ggcatcctct
     4561 acgggatgtc cgagacgcgg ctggcccggg agcaggggat cccacgtgcc gaggcgcgcg
     4621 gcttcatcca cgcctacttc gagcggttct ccgccgtgcg agcctacatc gaccgggtgc
     4681 gcgaggcggc cctgcgcgag gcggccgtcc gtacgttgtt cggccgggtt cgctacttcc
     4741 cacagttgca tcagcgcgtg aaccgcgcgg tccaggagca ggcgttgcgc gcggcggtca
     4801 acaccacgat ccaggggacg gcggccgacc tgatgaagat ggcgatgctc gccgtcgatc
     4861 gccggttgca ggcggacggc ggcccggcgc ggatgctgct tcaagtgcac gacgagcttc
     4921 tgttggaggc gccggcggat cggatcgact cggtggcgcg tgtggtgcgc caggcgatgg
     4981 agagcgtcca cccgctcaac gttccgctgg tcgtcgatca aaaacaggga gcgaactggg
     5041 aggacgccag ttgagcgcgc cgcgcgtagc gcttgggctc gttcggcttc cgcgataaga
     5101 tggcttctcg acgagatcga ggtatccggt tgatcgaagg cagcgtccct gcgcgaaagc
     5161 gtctgctcgt cgtcgacgac gagctgtccg tccgcgagat cctctgcgag ggtctgaccg
     5221 agttcggctt cgacaccgcg acggccgccg acgcggccga ggcgttgaag atcctcgccg
     5281 accggccgat cgatttggtg ctgaccgaca tcgaaatgcc cggggcgagc ggtatgacgc
     5341 tgttggccga ggtcaaacgg cgtacccccg acctcgacgt gatcatggtc accggcgtcg
     5401 tcgacacgcg gaccgcgatc ggcgcgatcc gcgagggggc cagcgactac ctgaccaagc
     5461 cgttcaacct ggacgaggtg cagatcgtcg tcgagcgaac gctggagaag cgacgcttga
     5521 tcgagcagaa tcgggcctac caggagcatc tcgaggacct ggtcgaggaa cgcaccgagc
     5581 agctcaggta ctcgtacgag agcacgttgc aggcgctggt caccgcgctc gattttcgcg
     5641 acaacgagac gcagggtcat tcggcgcggg tcgtcgaata cgccgttctc gtcgcgcgac
     5701 agatgggaat cggtgaaccc gagctgacga cgatccgctg ggggtcgatt ctccacgacg
     5761 tggggaagat cggcatcccg gaccgcgtgc tgctcaaacc cggcacgctg tccgaggagg
     5821 agtgggtgac gatgaagcgc caccccgaga tgggctaccg catgttacag cacatcgcgt
     5881 tcctcggtcc ggcgctcgac atcgtcttca gccaccagga acgctacgac ggcaccggct
     5941 atccccgcgg gctgagggcc gaagagatcc cgctcggagc gcgtgtgttc gccgtcgtcg
     6001 acaccttcga cgcgatgacc tccgaccgcc cctaccgcca ggggctgtcg atcgaggccg
     6061 cacgcgccga gatcgagcgc tgcagcggga cccagttcga cccgcgggtg gcgcacgcat
     6121 tcctgtcgat cgcggccgat cgctggcgtg agatccgcac gcgcgtccac gaagagacga
     6181 tggcgatcga agagcgggtc cggcgcgtga tgggttccga ttcccgagac gactcggttc
     6241 agggcgcggc gggcagcggc tcgctgccgt ccccttcgta gatcacgacc gacttgacca
     6301 ggtcggacgg tagcatcaac gccaccacgc cggccaggtt ccgcacgacc cgggcgaagc
     6361 cgtcgacgcg tcccagcagc tccggtcgcg gtctgagcga gatgaaccag ttcccgtcga
     6421 gcggatcgag gccgtccgcg ccccaggcca gtgtcccggc cagtacgatg tcgttgccga
     6481 tttcctcgcg caggtcgaag ccgcggtcgg gcaccgcctc ggccgccggg gtgccggcga
     6541 acagggcgac gtcggcgacg agccgcgaga tcggcagccc gtcgaacgct cccgattcgg
     6601 ccagcctcgc gaactcgcga ctggcgagcg gggcgttcgc cgtatcgagc gcgatgctga
     6661 accgtcccgg ctcgaaaccg cgtcgctcga cggtgacgat cgccgcgcgc ggcttggccg
     6721 cccagcgcaa caatgcgacg tagtgctcga ccggatggtc cgtcgccggg ccgagtgact
     6781 gacgccggtc gtccccgtag gcgctgaaca acagctgcgc cgcgacgacc cgcggtcgcc
     6841 aggcagccga ttcgagcccc gcctcgagca acgcgcgcgc ccggtcgtcg ggtcgggccc
     6901 ggccggtggc ccggagcacc gccacgccga gcgcgacgtc ggacgactcg ccggcgtccc
     6961 gccacagccg ttcgagcagc gcgaacgcgt cgtcgatttc ggcgctcgca gcctgctcgt
     7021 cggcgagcac gcggccggcc gccagcgcca gcgcgcggcg gattcgcgga tcggcggatt
     7081 cgaccgatgt cgccagattc tccgtcagca agccgtcgac ctcggcgaag accgggatga
     7141 tcgccgcccg caccgacggg tcctggtcgt cgcgcagacg tcgggcgaac tcgatcgtct
     7201 cgtcgtcgat ccgcccgatc aacaggtcgg ccgtcgcccg ccgcaccgcg gcgtggtccg
     7261 agcgcgacag gccgttcgcc atcttcaccg agtagttggt ggcctgaccc agcgcgacga
     7321 ccgcccgctc cttgatccac acccgcgggt cgaaaacgac gatgtcggcc agggtctgga
     7381 cgatgtcggg gccgcgcatc cgaccgaggc cgtcgacgaa ctcgagatag acgcgcgcgt
     7441 cttcttcctg gcccttctcg aggatcttgc tcagaaacgg ctcgagcggt gcacccggaa
     7501 agctgatcgc gcgaatcgcc tcgatccgga cggacgaatc gcgatcatcg accagcgccc
     7561 cgaccgaggc cgcttcctcc tcgcgcgtcg gccgcaccag gccgcgcgcc gccatccgtc
     7621 ggagcacggt gtatcgcgac gcggtcagct cgatcatccg gtcgcgggcc gcaacgcggt
     7681 cctcccgccc gatccgctcg ggctccgccg agagcatcga cagcgcccag ttgcaggcga
     7741 agatcagctc gtcccggtcg ctccgaccga gctcgatcaa gctccggacg gcgaaggccg
     7801 gccgcgcgaa gcgcgggacc gcgagacagg ctcgagcggc gacggccgga tcggggtcgg
     7861 cgagcagccg ggtgaccgca ggttcggcat cggtcgtggc gatcagcccg agcgcgtccg
     7921 cggtcgccgc gcgcacggtc gccgattcgt cctgcccgag cggctcgagt cgttcgagtg
     7981 cgccggccgc tccgatccgg ccgatcgcga acgcggccgc cgcgcgcacc tccggcgcgt
     8041 cgtcctgggt cgcctcctcc agtcggctcg actgtcccgg gtccccgatc cgcccgagcg
     8101 cgcgcaccgc cgccgcgcgg atctccggtt gcgcgtggcg gagggcggcc aacagctggt
     8161 cgtcgaccag ccgccggtcc tcggcccgca ggacctgcgc ccgtaccgtg agcagttctt
     8221 cactactggc cggcgcagac gacgcgggag ttcgtccggc cgcgccgcat cccgacgccg
     8281 ccacgggcaa gaggcataac agtatcgtcg acagggctcg aaccatcgtc acgattctcc
     8341 tttgctaccg gcgtcgccgc cgggcccgct tgacggtccg gcggaccgct gttagctttg
     8401 gaaacgctcg aataacctag ccgaccggcg gccgagcccg ccagttccgg tcggcttgcc
     8461 ccgctccggc gtcagcgact ctccgcgcgg atcggagagg aggctcgtgt tcatgagcaa
     8521 cggtcgtcac caggtgtcgc gcaagctctc gctgctcgtg ctcggttcgc tggtcgcgac
     8581 tgccgccgcg gcggacgacg acctcgatcg cgtgctggcg cgcttcgacg acgtgcaagg
     8641 atcgatccag tcgctttccg ccgacttcac gctgaccacg acgaaccagc tgctgctcga
     8701 tccgatcgcg gcccaaggtc gcttctacat gaccaagccg gattcgatcc gctgggagta
     8761 cagccaaccc gaggagatgc gcttcgtcat cttcggcgac cagtacaccg ggttcttccc
     8821 ggaacagaag cgtgccgagc gcaagaacat ccagcgctgg cgcgagcaca tcttccgctt
     8881 cttcggcttt ggccagggga cgagcgagct gcgcaagttc tacgacatct cgctcgaccc
     8941 cgaccacgat ccgtcgaacg gcacctacct gctgatcctc gagccgagca agaagcgcgt
     9001 gcgcaagcga atccccgagg tccggttctg ggtcgaccgc acgagctacc tgccggccaa
     9061 ggtcgagtac gtcaaccaga acggcaacac cagcctgctc gagctacgcc acattcaggt
     9121 caatcccgac ctggcggcct cgacgttcca ggtcgaggta ccgcgggatt tcaaggtcac
     9181 caagggtttc agcggtctgc ccgatttcga caccagctcg ggcgagtgac gtcccgcacc
     9241 gatgagcccg tcggcctgat tctcgacccg gtgttcgagc ggcacgagac cggcccgggt
     9301 catccggagc gctcggcgcg tctggtcgcg atccgcgagg cgatcgaacg cgacgccctc
     9361 gattgccggg cggaacgggt cgcgctcgaa tcggccgacg atcggctgct cgagctggtc
     9421 cacgatgcgg ctcatctgcg ccgtgtgcac gaggcgtgcg agtcgggcga gccgacgctc
     9481 gactcgatgg ataccgcgat ctgcgccgac agcgaccgcg tcgcgcggct cgccgccggc
     9541 tccctcgtcg cactctgtcg ccgcgtcgcg cgtggcgaac tgtctcgcgg attcgccgcg
     9601 atccgcccac cgggacacca cgccgagcgc gacttggcga tgggcttctg cctgttcaac
     9661 aacgtcgcga tcgcggcgcg ctacctgcgc gagcacgagg gcgtcgcgcg cgtgctgatc
     9721 gtcgactggg acgtccatca cggaaacggc acgcaacacc tgttcgaaca ggacccgagc
     9781 atcttctttt tctcgttgca ccagtggcca ctctaccccg gcaccggcgc ggccgaagag
     9841 cgcggcgtcg gcgccggcct cggcaccacg ttgaactgcc cgctcgcgcc cggtgcgggg
     9901 gacgccgagt tcttgttcgc gctacgcgag gtgatgctgc ccgcggcgct cgattttcgt
     9961 ccggaattcg tgctggtgtc ggcgggcttc gacgcccact acgccgatcc gctggctaac
    10021 ctcgaggtgt cgaccgaggc ctacggcgag gcgaccgaca tcgtctgtga cctggccgac
    10081 cgcgtcgcgg ccggccgggt ggtctcgacg ctcgaggggg gctacgacct gcgcgccctc
    10141 ggcgagtcgg tcgcgctgca cttgcgccgc ctgcagcggc tcgcctgacc cgttaccacc
    10201 cgcagctccg ttcccggttc tgctcgtcga gccaggcgat cagcggggcg aagtagtcga
    10261 gaatcgcggt cgcatccatc tcgcgctgcc cggtgagcgc ctcgagcgcg tccggccacg
    10321 ggcggctcgc gcccatgcgc agcatctcga tcaggcgccg gccggcctcc ccgtcggcgt
    10381 agatcgagca gcggtggagc ggcccgtcgt ggcccgagat cgcgcacagc gctcggtgaa
    10441 actggaactc gaggatgtag gcgaggaagt agcgcgtgta gggtgtgttg gccggaacgt
    10501 gatacttggc cccgggatcg aaatcggctt cgctgcgcgg cacgggggag gcgatcccct
    10561 ggtaccgctc gcgcagctcc caccaggtcg cgttgtagcg ctcggcgggg atctcgcccg
    10621 agaatacctt ccagcgccac tgatcgacca gatagccgaa cggcaggaac gcgatcttgt
    10681 cgagcgccgt gcgcatcagc aggccgaggt cgccggccga gccgggttca cggtcgagga
    10741 gtccgagctg cgccaggtac tccggagtga tcgacagcgc gatcgtgtcg ccgatgccct
    10801 catggaaccc gtcgttcgcg ctgttgcggt agagcgggtc gagcccgccg taggcgcgct
    10861 ggtagaaatt gtggccgagc tcgtggtgga cggtgatgaa atcctcggcc gtcacgtcga
    10921 tgcacatctt gatccgcagg tcgtcgaccc agtcgatgtt ccaagcgctg gcgtggcaga
    10981 cgacgtcgcg ttcggcgggc tgggtgaaca acgaccgttc ccagaagctt gccggcaacg
    11041 gctcgaagcc gagcgaggtg aagaagcgct cggcgatacg caccatcccg accgggtcga
    11101 cctcggccgc caccagcaat tcggtcaagt cgacgccggc gtcgccgccg gccggaccga
    11161 ccaggtcgta gacgttgctc caatcctggg cccacatgtt ccccagcagg tgggccggga
    11221 tcggctgtcc gggagggacg acctgctcgc cgtagtaatc gctcaaccgc gcccgcacgt
    11281 ggcagtgcaa cgcgtcgtac agcggccgaa cctggcccca cagccgctcg acctcgtcgg
    11341 cgaaccgctc cggcggcatg tcgtatcccg agcgccacaa cgcccccaga tcgtcgaacc
    11401 ccagcgctgt cgcgccggca ttggccagcg agacgaaccg tacgtacttg tcccgcatcg
    11461 gcggcgagat cgtgcgccag ccgctccacg cttcgagcaa ccgagccggg tcgcggctgg
    11521 aagcgagcgt ccggctgagc tgtccgagat cgaggcaatc ggccccgtcg ccatccgggc
    11581 agtagcggcc cttgccgtag atactggaca acgaggccgc gatcgccgcc agctcggcct
    11641 gcagcgcggc gtccggcggg ccgacaagcg gcagcgaggt cttcagcatg cggatcttgc
    11701 ggctcaattc gtccggcagc gcgacgccgt cgaaccgtgt cgacgcgagg gccagctcga
    11761 ccgtcgtcgc gatccgctcc tgcgcggcgt cggcggcgat ccgctccgta tcgacggtga
    11821 tgaagttctc ctggacccac gacgcacgct cggccgcgat ccagcgatcg agcaggcgcg
    11881 cctcggcgtc gccgacgaac cgagcggcgc cctcggccgt cgccgggtag tcgggcgggt
    11941 cgtggtccgc cggtgcgcag cccgccgacg acagcaacgt caccgacgcg aaggcgaccg
    12001 gtaaccaaat cgatcgttgg agccggtacg gtctcatcga gcgtgactcc ggtcgagcat
    12061 ctctcgacgc gtggccggct cgagcctagc acgtcgggcg cacttgccgg tcgatttccg
    12121 acgtggttca atggcgcgac cggaaacgga gcgacgaccg atgggcgacg gcaagacccg
    12181 ctacgacgac tacctcgccg aggagttgaa gaacttcgag gagctggcga agttcctccg
    12241 gccgtcgccc ggcggcatcc cgcggctgga cggcatcgac atcggcggtc tgttcatgcc
    12301 gctgtacggc gtgatcggcg gcgacctggt gacctacatc gacttcagca gccgctacga
    12361 cttgccccgc cgggtcgagc gggcgcggag cgccgggctg accgcggtgg ccgagaagct
    12421 gtcactgcta cacgagcggg ccggcatcct cgtcgcagac atctccggtc accgtatcac
    12481 cgatgcactg atcgcggcga tgctgcacca ggcgttcctg ctcggcgcgt actacgaact
    12541 ggacatgttc ggcgagatca ccacccgatt gttcgaacag ctcaatcagc gtttctaccg
    12601 cacgaccacc atcaacaagt acttgacgat gatctacggc gagatctcgc ggcaggggac
    12661 cttccgcttt ctgtcggccg cgcaccagcc gccggccgtc ttctcgcgcg agtacggcca
    12721 gttcgtgacg atcagccccg atcggctcgt caccctgcca ccggtcgcga tgttcccctc
    12781 gcgcgccgac cccgacgcga gcaaccaccc caatctgatc ggttacaagg agcggtacga
    12841 ggtcaacgag atcaacctgc tggccaaggg cgacatcctg ctgctgtcga ccgacgggtt
    12901 cgccgagcac gccgacggga gcttctttcc ggagaaggtc cggcagctgt tggccgcatc
    12961 cgacgggtcg tcggcgagcc agatctgcga ccggctgcgc caggcgatca tcgcggccgg
    13021 cgagcagacg gacgacctgt ccgtcgtcgt gatcaagaag accggctagc gccgttccgg
    13081 cggccggccg catccgggaa cggcaaatcg accaccctcg cccggagcgc cgactcgctc
    13141 gcgccgacga acagctcggc ccccgcgccg gccgccctgc gcttgaggta gccgatcgct
    13201 atcgaacgat tccggccggg cggcgtgacg gcgctggtca acacgccggc ctcgcgcccg
    13261 tcgtgataca acggggtgcc cgccgcgggc agttcgatcg atcgatcgaa caccaaaccg
    13321 accagcgagc gagagacctt gtcgtaggta ttgagacgag ccacgacctc ctggccgacg
    13381 tagcagccct tggcgaacga cacggcgtcg tacagcccgg cctcgagcgg attgtagtct
    13441 tcggtcaact cgtggcccgg tgccggcagc ccatgcaaca cgcgccaggc ctcggcggcg
    13501 ctcgtgcccg ctgggagacc gccgcagcgc tcggtcgcct cgcgcaacag acgccatgcg
    13561 tcgcccgcga ccccggccgg caggaagacc gagtagccgg cggtcgacag cccgtcctgt
    13621 cccagcacgt gcatctcgac gccgcccagc tcggcgacga cgctggccgc cgggagcggg
    13681 cggggcagcc cggccgcggc gagcgcctcg gccgcgcggg gaccgacgac cgacaactgg
    13741 ccggtcttcg ccgagacgtc gtccaacccg gtctgctcgg cgaaggtgaa tcgatcgagg
    13801 tacgcgacca ctcgcggcgc ggcccccgcg cccgagacca gcagcacgct ccgatcggcc
    13861 agccggtgga cgaacaagcg ctcgacgatc cgtcccttgg ccgtggtgac gacggtcgtc
    13921 accccttggc cgaccgccag cgaatcgagc ttggccgtgc tgaggcggtt cagcagcgac
    13981 agcaggtcgg gaccgctggc cagcaggcgg ccgagcatgc cgcgatcgac gagagcgcac
    14041 cggtcgagcg cggcgtccaa ctcggcggcg ggtcggccga agtgcagtgc ctcgccgctc
    14101 agcgcgtcga acacggcgcc actcgcttgc agccggtcgc gcagtcctcg atccactcgc
    14161 tcgctcagac gaactcgatc gtgtcgacgg gcgggacgac tccggcggcg cagccctcgg
    14221 cgaggaagcg ccggatcgct tcccgtccgc gctcgccgta atcgcgcgtc aagtcgttga
    14281 cgtacatccc gacgaaccga tcggtcagct cgcggtcgag accccggccg aagtcgagcg
    14341 cgtaggccaa ggccggatcg cgatgacgca acccatggtc gatgctctgc tcgagcattc
    14401 gggtcagtgc gaccatcgtc tcgcgaccca gatcccggcg cacgacgttg ccgccgagcg
    14461 gcaacggtag accgccggtc cgctcacccc accaccggcc gaggtcgacg acgagtcgca
    14521 gcccctcgcg cgggtaggtg atctgaccct cgtgtatgat caagccggcg tcgacgcgac
    14581 cgtcgagcac ggcctgcggg attcgatcga acggtagcac gtcgtagcgc agcggcccgc
    14641 cgaccaacag ccgcagtgcg aggtaggccg aggtcagggt tcccgggatc gcgatcgtcc
    14701 tgtgggcgag ctcgccgggg tcgaacgctc ggcgggcgac gacgcacggg ccgtacccgt
    14761 ctcccatgct ggccccgcag tgcatcagcg catagcgttc ggccaggtgc gcgtaggcgt
    14821 ggatgctcac cgcgctgacc tcgagctctc cgcggagggc gcgtcgattc agcgattcga
    14881 tctcctcgag cacctgctcg acgcggaacg gtccggtgtc gaccgcgccg gtcgcgatcc
    14941 catggaacat gaacgcgtcg tccggatcgg ggctgtgacc gatacggacg agccgggtct
    15001 cggtttgctt caaccgcgtt tctcccgcga gctcgatcgg ggttgcggat tataaccgac
    15061 gcgcccgacc tcgccacttg ccccgatcgc cgcggccgtg gagaatgggc agtcggaccg
    15121 aacgagccga cgagcacgga ggagaaggtg agccgacgga gccggaggtt gacgggaatc
    15181 gcggcgctag tcgtcgtcgc cgcaggagcg ctggccgccg cgcccgacga cgagcccgac
    15241 gtcgtggttg tccaacccgt gctgatcgcg ttcaagggaa gtctacccgc caagcaagtc
    15301 gatcgatcca agaaagaagc gcgagcgctg gccgaggagc tgttcgagca ggcgcaggcc
    15361 gaggacgccg atttcgacgc aatggtcaaa cagtacacgt ccgacagcta ccccggcgtc
    15421 tacaagttga ccaaccacga cgcgccgatc accggcggcg cctaccagcg caaggagatg
    15481 gccacgcgct tcggcgacgt cgcgtttaag ctcgaggtgg gagaagtggg gctggccgcg
    15541 tacagtgtga cggcaagccc gtacggctgg cacatcatca agaggctgga atagcgttgg
    15601 ccgacgatcg tctcaggacc gaggccgatc ggatcgcgcc cgagctcgtc gtttggcgcc
    15661 ggcggctgca tcagcagccg gagctcggct tcgaggagca cgagacctcg cgcttcgtcg
    15721 ccgagcggtt ggcgcaactc ggggtcgagg tccgcaccgg tctggccggc accggggtcg
    15781 ccgggatcct gcgctcgccg cgccgcgacc ggccgggggt gctgctccgc gccgacatgg
    15841 acgcgcttcc ggtgcaggag gtcgacggcc gggagtacgg atcgcgcgtg ccgggcagga
    15901 tgcacgcctg cggccacgac ggccataccg cgatgttgct cggcgcggcc aagctgctcg
    15961 aagcacgcaa gagcgagctg aggcgcgacg tggtgttctg cttccagccc gccgaggagg
    16021 gtctcggcgg ggccgagcgg atgatcgacg acggtgtgct cgagctggtc gagaccgggt
    16081 cggtgttcgg gctgcacctg tggtccgggg ccgaggtcgg cacggcgatg atgcgcgccg
    16141 gtccggcgat ggcggcgcaa gacgagatcg aggtgcggat cctcgggcgc ggtggacacg
    16201 gcgcgctgcc ccacacggcg cgcgacccga tcgtcgcggc gtcgctcgcg gtggtcgcgc
    16261 tgcaggggat cgtcgcgcgc aacgtcgatc ccgtcgagac cgcggtggtg acggtcggct
    16321 cgctgcacgc cggcaccgcg cccaacgtga tcccggcgga ggcgcggctg gcgggcacgc
    16381 tgcgatcatt cagcgaggag gtgcgcgagc tgctccgcct ccgctcgcgc gaggtgatcg
    16441 aatcgagcgc gatcgccgcg ggctgcacgg cggactatct gctcaagaag ggattcccgg
    16501 cggtggtcaa cgacgcggcg tcggtcgaga tcgcgcgacg gctggcggtc gacgtgttcg
    16561 gcgagggcaa cgtggtcgag cacccgccga tcgccgcggc ggaggatttc tcgtatttcc
    16621 tcggccggcg tcccggagcg ttcattttcg tcggcgcggc caataccgcg cgcggcatcg
    16681 acgcgccgca tcattcgccg atgttcgaca tcgacgaggc cgcgctgccg cggggagcgg
    16741 agctgctcgc gcggctggca ttggattgag atcgagccag atcggcgatc gatggcggag
    16801 gaagcccgat ggcgaagacc aagaccgagt tcgacaaacc gcaggtcaag gccgcgatca
    16861 aagagctgaa gcagcggcgc cagcgcgccc tcgaggccaa ggaccgcgag gagctgaaga
    16921 aggtcaggcg acggatccat cgcctgaagc acgagctgcg cgcgagtgtt cggacacgct
    16981 cctagccggg cggtggccgc cggtctcgga tcgcccttcg ctgcgtcgcc gccgggactt
    17041 tgttccgcct agcgggcgta ggggttcttc gctccgccgg gtggtcggat gcggaagcgc
    17101 ttgtagctcc agatgtactg ctccgggcac cggcgcacgc acctctcgac ctcgcggttg
    17161 agcgcggcgg ccgaccgctc gagatcggcg tcgccgattt cgtcgctcgt cgtgatgaag
    17221 tgcaggtcga agccgcgccc gcggggcaga cgctcggcgt agcccatcac caccagcgca
    17281 ccggttcgtg ccgcgagctt ggacaacagc gtcatcgtgt tggcctgaac gccgaagaac
    17341 gggacgaaga tgcccatgcc ctcggccggc tcctggtccg gcaggatcgc caccaactct
    17401 ccgcgtcgca gcgcggcgag caacgtgcgc accgcggtca gccccgacgg aaccagctcg
    17461 gcgcccattc gctcgcgccc gctcttgacg agcggctcga gctccgccaa ccgaggtcgc
    17521 cggtagagcg tcgtcatcgg gaagcgcttc gacacttcga tcccaccgat ctcccagctt
    17581 cccagatgtg gcgcggcgag aatcacgcca cgcccgttcg cgagcgcgcg acggatcgcc
    17641 gcgtcgccgt gcaccgtacg gacgtgacga ccgatccgct cgcaactcca gcaccacgcc
    17701 gcgctgaact cggcgaacgt cttcgccgtc tcgatcatgc tgcgccgcgc gagacgccgt
    17761 cgctcggcgg gtgccagctc cgggaacgcg atgccgacgt tgatctcggc gacggtgcgc
    17821 tcggggatcg gcagcaggcc ggcgatccga ccgatcgtcg cgccggcggc ctggatcgtc
    17881 cacagcggca gcatcgcgaa cagacgaatc agatgagcga tgacccacgc gacgagcggt
    17941 cgcggcggtg gggcggtcgg cgagccacgc ccacggcgca ggcgccgggt ggccggcgca
    18001 gaactggcgc tcatcgcggc atcagcgacg ccgagctgga ggaaaggccg ctccgcacgg
    18061 tcaggacgat cccccgccga cgcgcgcgat cgtcccggtc gtgctgtgtc cttcctcgag
    18121 tttggccaac agcacgcgtc cgccatagct cgtcaccagg tccgcgccga cgacgcggtc
    18181 tttggtgtag tccgcgccct tgaccagcac gtcgggtttg atccgctcga tcaagcgcaa
    18241 cggcgtgtcc tcgtcgaaca cgacaaccag gtcgaccggc gcgagcgagg ccagcacgac
    18301 gctacgggcg gtctgcgact gaatcgggcg cgatggtccc ttgaggcgtc gcgcggacga
    18361 gtcggaattc agcccgacga tcaaccggtc gcaggcggcc ttggcctgat tgagcagcga
    18421 gacgtggccc gggtggatca gatcgaagca gccgttggtg aagccgaccc gcaggccctg
    18481 cgcacgccag cgatcgacca gctccagcgc ctgtttcaac gtgacgatct cgccgttgac
    18541 cgagtcgatg tcggcgcggt ggatctcggc caccagatcg cgcggataga ccgtcgcggt
    18601 gccgaccttg ccgacgacga tcccggcagc gacgttggcc aggcgggccg cgtcggccag
    18661 ttcccaaccg ctggccaacg cgaccgccat cgtcgccgcg acggtgtcgc cggcgcccga
    18721 tacgtcgtgc acctcgcgcg cacgagccgg tagttggacc ggctcgccgc ccgacgtcac
    18781 gagcgtcatt ccccgttcgg atcgcgtgac caacaggttg tcgactcccg cttgttcgac
    18841 gacggagcgc gcggccgcga cggcctcgtc gtccgtgtcg cagggcatcg cggttgccgt
    18901 gcgcagctcg ccggcgttgg gcatcagcag atagacaccg cgatagcgcg agaaatcgcg
    18961 ggtcttggga tcggcgatca ccgtcttgcg tcgggaccgc gccagatcga tcgccggccg
    19021 cagcaccgcg tcggacaaga cccccttcgc gtagtccgac aggacgacca agtcggcatg
    19081 ctcgagctcg gcctcgtaga tcgacaacag gcgttcggcc agttcgccct cgatcggccc
    19141 ggtatgctcg acgtcggccc gcagcatctg ctggttatgc acgacgaacc gggtcttgac
    19201 ggtcgtcaac cggtcactgc acggcacgag gtgcgaacgg aggcggtcct gctcctcgag
    19261 cagccgtcgc agtaccgctg ccatctcgtc ccgtcccacc acgccgatga ccgtaccgag
    19321 agcgccgagc gtcgtcacgt ttcgcgtgag gttgccggca ccacccggca tcagcagccg
    19381 ccgtcggaca ttgaccaccg ggatcggcgc ctcgggcgag agtcggacgg cgtcaccgta
    19441 gacgaactcg tcgagcatca cgtcgccgag gcacaacaca cgggccctgc cgaagtcgtg
    19501 gatgcggtgc ctcaacgcgt tcagctctgt catcgtcgct cccgttcgag gatcgcccgc
    19561 gcctccagtg cgccacacag cgtgtggccg aggagcaggt gtacctcctg aatgcgagcg
    19621 gtcacgtcgg acgggacgac cagcgccacg tcgaccaacg ggaccaactc gccgccgtca
    19681 ccgccggtca gcgccacggt ctccaggccg agccggcgtg cggtgcggaa ggcgagggcg
    19741 acgttggggc tgcgacccga ggtcgagctg ccgatcgcca cgtcgccagg acggccgagt
    19801 gcctcgatct ggcgcgcgaa gacctgctcg taacccaggt cgttggcgca cgccgtcagc
    19861 gcggagctgt cggtcgtcaa cgcgatcgcg gccaacgccg ggcggtcggc ccggtagcgc
    19921 acgaccagct cggccgcgaa gtgctgcgcg tcggcggcgc tcccgccgtt gccgaaaaac
    19981 agcagcttgt ttccgtggtc gagtgcgtcg gcgcagatcc gcgtcgccgt ggccagccgc
    20041 tcgcccaact cgtcgccgac gagccggacg atgtcgaggt gctgtcgcag ctcgcgctcg
    20101 aagtagtctg ctacgtccaa tcggatcgtc tcccaaaccg cccgacgcag gacgaccggt
    20161 ccattatgcg acaggccgac ccgttcgggc gactgcgctg ctccgcgcca ctccagtcta
    20221 cgaacgcgcg gcgggggttc gccagggccg cggccaccgc cggtggtgcg gtcggacccg
    20281 tgctatcgtc gcagcgtctt ttccgggggt ttcgcgtgaa cgtcgctgtg ggtcgcgtgg
    20341 acggcaccgc ggcgcaacgg cctccgccgt cggttcgggc gatcgtcgtc gcgatctggg
    20401 tggcgctcgg ttcggccgcg gcctcgccgg atccgccctg cctgaacgac gggccgcatc
    20461 ccggtcggac cgagcagtgg attgccgcgg cgatcgacgt gctgtccgcg gccggacacc
    20521 caccgcggcg ctaccggctg gagttgagga tggagcccgc cgcgcgaccg gactttcccg
    20581 aactcggggt cgagcgcgta cggagcgtcg tgttcgtccc gcgcgatccg acgacggact
    20641 acccgctccg cgtccatccg tcgcgaccct gctcgctggc gtgggtctgg cagcccggga
    20701 gcttcaccga ctggcaacgc gcggtgatcg accgcgcgca ggcgatctcg gccgcgcgcg
    20761 ccgcaggcga ggtgcagcag gtgcaagtga tcgagaccgc ccggtcgatc gagatcctgg
    20821 tcgtcaccgg cgacggcagc tccgagcgca tcgtcttcga aaaaagcga
//
LOCUS       DASPGN010000230         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_156094, whole genome shotgun sequence.
ACCESSION   DASPGN010000230 DASPGN010000000
VERSION     DASPGN010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156094"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VD788_06660"
     CDS             complement(<1..416)
                     /locus_tag="VD788_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV75988.1"
                     /translation="MSQLLSIVIPAYNEEKTIAELLAAVLAAPLPDGIERELVVVDDC
                     SGDGTAAELGKFTGDPRIRVFHQPVNRGKGAALHRGFAESRGDFVVVQDADLEYDPRE
                     YPKLLAPILAGKADVVYGSRFMGGEAHRVLFFWHYVG"
     gene            complement(478..1611)
                     /locus_tag="VD788_06665"
     CDS             complement(478..1611)
                     /locus_tag="VD788_06665"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV75989.1"
                     /translation="MDRPRLTVVTDADAAGVGTRAPVPLVGLLVILAAAAASGLGADR
                     ARVKPEPREIARRALATAMEHGSDAAAVRDDLTRLREDLARRPLDSATRVAYASLVLG
                     LARGPDDLAVADFHARLATRISPVTVPVVRAAATVLARSGGPEETVRLIHEMFQYDAA
                     AAARLLGQLGFLLTEEQSAAAVADRPEAWLAWARRLREIGRGEEADRWVVRGNARWPD
                     DLGLLVPAVEQAARAGDWARVARLLPADRTIPLVPDAAILYAYRSRARNRAGQTAAAV
                     EDAATAVRVGRDYATVLAYAGDAYAELGDAVEARTVWSRALFLTPPDATDYRLRILLS
                     LARLEERDGRPATALRHWRSVLVVDPDNDEARRRIAALTGARP"
     gene            complement(1593..>2235)
                     /locus_tag="VD788_06670"
     CDS             complement(1593..>2235)
                     /locus_tag="VD788_06670"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXV75990.1"
                     /translation="GLLLVVGVAGLFASFSRAGISLGVAALAWTALAAGRFRTVRTRV
                     VVVALLLGAASVPLAGIGADRLLARFGDAAFDLQDSGGRLTVWGDTRRMIGAFPICGT
                     GLGTFAEIYPRYRSPEVRKFYSHAHSDPLQLVAETGAAGALLALGAAVPLLVVVVGAL
                     RGRKGTVGIGFAAGFVALSLHSSIDFNFHLPANAGVAAIIAGTLLGLPWIDPD"
BASE COUNT          353 a          818 c          795 g          269 t
ORIGIN      
        1 ccgacgtagt gccagaaaaa cagcacgcgg tgcgcctcac cccccatgaa cctcgacccg
       61 tagaccacgt cggccttgcc ggccaggatc ggggccaaca gcttggggta ctcgcgtggg
      121 tcgtactcga ggtccgcgtc ctgcaccacg acgaagtcgc cgcgggactc ggcgaaaccg
      181 cggtgcaacg ccgcgccctt gccccggttg accggttgat ggaagacccg gatccgcgga
      241 tcgccggtga acttgcccag ctcggcggcg gtgccgtccc ccgagcagtc gtcgaccacc
      301 accagctcgc gctcgatccc gtcgggcagc ggggcggcga gcaccgccgc cagcagctcg
      361 gcgatcgtct tttcctcgtt gtaggccggg atgacgatgg agagtagttg ggacaagtcg
      421 gctcccgcgt ttcacacgag gcgtgagagt atgcgaccgt cgaggtacgg tcaactccta
      481 cggcctcgcg ccggtcagcg cggcgatgcg gcggcgcgcc tcgtcgttgt cgggatcgac
      541 gacgagcacc gagcgccagt gtctcagcgc ggtcgccggg cggccgtccc gctcttcgag
      601 acgcgccagg ctgagcagga tcctaaggcg gtagtcggtg gcatcgggcg gggtgaggaa
      661 cagggcgcgg ctccagacgg tgcgtgcctc gaccgcatca cccagctcgg cgtatgcgtc
      721 gccggcgtag gccagcacgg tcgcgtagtc acgaccgacg cggaccgccg tcgcggcgtc
      781 ctcgacggcc gctgcggtct gccccgcgcg gttccgtgcg cgcgagcgat aggcgtacaa
      841 gatcgcagcg tcgggcacga gagggatcgt ccgatccgcg ggcagcagtc gggcgacacg
      901 cgcccagtcg ccggcgcggg ccgcctgctc gaccgccgga acgagcaggc cgagatcgtc
      961 cggccaacgc gcgttgcctc ggacgaccca acgatcggcc tcttcccccc gcccgatctc
     1021 ccgtaagcga cgggcccagg ccagccacgc ttccggccgg tcggcgaccg ccgccgccga
     1081 ctgttcctcg gtgagcagga agccgagctg acccagcagc cgcgccgccg cggcggcatc
     1141 gtattggaac atctcgtgga tcagccgcac cgtctcctcg gggccgccac tgcgggccag
     1201 gaccgtcgcc gcggcccgta cgacggggac ggtgaccggc gagatgcggg tggcgagtcg
     1261 cgcgtggaag tcggccaccg ccagatcgtc cgggccccgg gcgagaccga gcaccaggct
     1321 ggcgtaggcc acgcgggtgg cgctgtcgag cggccggcgt gcgagatcct cgcgcagcct
     1381 cgtcaggtcg tctcgcaccg ccgcggcgtc gctgccgtgc tccatcgcgg tcgccaacgc
     1441 gcgacgtgcg atctcgcggg gctccggctt cacgcgggcc cggtcggcac cgagcccgct
     1501 ggccgccgcc gcggccagga tcaccagcag accgacgagc ggtaccggcg cccgcgtgcc
     1561 gacccccgcc gcgtccgcgt cggtcacgac cgtcagtcgg ggtcgatcca cggcaacccc
     1621 agcaatgtcc cggcgatgat cgcggcgaca cccgcgttcg ccggaagatg gaagttgaaa
     1681 tcgatcgacg agtgcaacga caacgcgacg aagccggcgg cgaagccgat gccgaccgtc
     1741 cctttgcggc cacggagcgc accgacgacg acgaccagca acggcacggc cgcgccgaga
     1801 gcgagcagcg caccggccgc gccggtctcg gcgacgagct ggagcgggtc gctgtgggcg
     1861 tggctgtaga acttgcgaac ctcgggggag cgatagcgcg ggtagatctc ggcaaaggtc
     1921 cccaggccgg tcccgcagat cgggaacgcc ccgatcatcc gccgcgtgtc accccagacg
     1981 gtcagccgtc ctccgctgtc ctgcagatcg aaggcggcgt cgccgaagcg cgccagcagg
     2041 cgatccgccc cgatgccggc cagcggcacc gacgcggccc ccaacagcaa cgcgacgacg
     2101 acgacgcgcg tgcgtacggt ccgaaaccgg ccggcggcca gcgcggtcca cgcgagcgcc
     2161 gcgacgccga gggagatgcc ggcgcgcgag aacgaagcga acaacccggc gacgccgacg
     2221 acgagcagca gtccc
//
LOCUS       DASPGN010000231         4452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_975941, whole genome shotgun sequence.
ACCESSION   DASPGN010000231 DASPGN010000000
VERSION     DASPGN010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975941"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VD788_06675"
     CDS             <1..196
                     /locus_tag="VD788_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75991.1"
                     /translation="KTVILINGGVFLVDEAGGSLAAHNTADREAVLRIDLRQAGRAWR
                     EEYVVPAGQAIEIEWPASAR"
     gene            complement(183..1163)
                     /locus_tag="VD788_06680"
     CDS             complement(183..1163)
                     /locus_tag="VD788_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75992.1"
                     /translation="MRLLALVDRETYLTKLSRVRFHSLEAIGRLCELRWSGPGWPGWD
                     VALTVDANVARSGWTPDLIEVYMPFGDQFAGNVSIKGIADTSIPVCLRYNEAFKVKRV
                     KNEIESCRADLVVFHHENEMRPYLSLRWKHGRVSCAHIPHSAEPSIFRPYGEPKTTDL
                     MLVGALGPRYPLRNRLAKLLPRFPRPYRAVAFEHPGYVHADAHTDRYAERFARAISAT
                     QICLSCSGRTRTRYGKYVEVPMCGTALAADMPEQDQDRIGKWLIELDLGMTDRQIIDK
                     LVAHLEDPREMSRRVEAGLAWARQYTQQHYAERFVAIAEEFVGQRRSSGR"
     gene            1286..1897
                     /locus_tag="VD788_06685"
     CDS             1286..1897
                     /locus_tag="VD788_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75993.1"
                     /translation="MASNRFLSTRTGWASSCALLATLAVCGCRGTTAPDETEPAEELA
                     IAADDVPREVRLAAGIALDALEAGAECRAFSWDREDDMWECTVDGLAFAAEPDLDRNG
                     AFSEFEFDVTLDVLDHHAPELASLIRDTCGRDGAAHVETSIRRVDLLGPDPSFDRLWG
                     EDAIFVEFQCADGTDYEVDAYGALVIAPDDDAEAPAVADPRVE"
     gene            2226..3254
                     /locus_tag="VD788_06690"
     CDS             2226..3254
                     /locus_tag="VD788_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75994.1"
                     /translation="MKKIQPGFILPSSAFTVSPRLIKGYEKLDRPPRVGDVVYGRISH
                     VGQHGSLENRQGRIHMISDGTRAVFVYGNRYAPDGFEGVLPETSLPEVDLLARSGLIG
                     TMRSKNTAIKDPTKVRILGHAIGEDLRPLNTIDHPIALPKSDVIDTKRRARMILNVGT
                     SMNSGKSTTAIACCWALSSMGHAVRAAKVTGTASLKDILHMQDAGAEKIADFTHLGYP
                     STYLLEIGTVIEIFRSLDAKFANNPKKFWVVEFADGILQRETAALLRDEYVRSRIHRL
                     IFSAVDAFGALGGLRALQEEFGLTPDAVSGRCTSSPLMVRELQGRTNIPVVNNVIRDM
                     KQLSEILL"
     gene            3251..4204
                     /locus_tag="VD788_06695"
     CDS             3251..4204
                     /locus_tag="VD788_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HXV75995.1"
                     /translation="MSVPTAVAVHGGAGRVGRERLTGPGRRRFFDGLSAALSAAHEIL
                     RRGGSAVRAVTTAVRVLEDDEVFNAGRGAALCADGSVELSASVMNGRNLAVGAMVGLR
                     RTRNPVLGAAALLDHKHGLLFGEQADNYARAAGLDQMETDYFVTPVRVRQWQALCGSK
                     RVVLDHSGDEEAHGTVGAVARDRRGNLAAATSTGGLVNQLPGRVGDSPVVGAGTWADR
                     RCAISATGSGDAFARIAFARRVADFVELAGMEPAAAARRALDEVARVHGRGGCVLIGV
                     EGAPVAVFNSPHMIRGWVVAGAEARMAIAPGDEAARRPGDR"
     gene            complement(4208..>4452)
                     /locus_tag="VD788_06700"
     CDS             complement(4208..>4452)
                     /locus_tag="VD788_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV75996.1"
                     /translation="SHPAGGEHRNALPLIDGAGPLAKRDSFLLRTDPEVLAALRRWAE
                     DELRSANAQLDFLLRRALREAGRLPAPERKHGGKGG"
BASE COUNT          688 a         1470 c         1610 g          684 t
ORIGIN      
        1 caagacggtg atcctgatca acggcggcgt ctttctcgtc gacgaggcgg gcggctcgtt
       61 ggcggcgcac aacacggcgg accgcgaggc ggtgctgagg atcgatctca ggcaggccgg
      121 gcgggcctgg cgcgaggagt acgtcgtacc cgcggggcag gcgatcgaga tcgaatggcc
      181 ggctagcgcc cgctagaccg tcgttgcccg acgaactctt cggcgatcgc gacgaaacgc
      241 tcggcgtagt gttgctgggt gtactgccgc gcccaggcca agccggcctc gacgcgacgc
      301 gacatctctc gcgggtcctc gaggtgggcg acgagcttgt cgatgatctg ccggtcggtc
      361 atgccgaggt cgagctcgat caaccacttg ccgatccggt cctggtcctg ctccggcatg
      421 tcggcggcga gcgccgtacc gcacatcggc acctcgacgt acttgccgta gcgcgtgcgg
      481 gtccgtccgg agcacgacag gcagatctgg gtcgcgctga tcgcgcgggc gaagcgctcg
      541 gcgtagcggt cggtgtgcgc gtccgcgtgc acgtagcccg gatgctcgaa ggcgacggcc
      601 cgataaggtc gtggaaagcg cggcaacagc ttggccaatc ggttgcgcag cgggtagcgc
      661 gggccgagcg cgccgaccag catcaggtcg gtcgtcttcg gttcgccgta cggccggaag
      721 atcgacggtt cggccgagtg cgggatgtgg gcgcagctca cccgcccgtg cttccatctc
      781 aggctcaggt agggccgcat ctcgttttcg tggtggaaga cgacgagatc cgccctgcac
      841 gactcgatct cgttcttgac gcgcttgacc ttgaacgctt cgttgtagcg caggcacacc
      901 gggatcgacg tgtcggcgat ccccttgatc gatacgttgc cggcgaactg gtcgccgaac
      961 ggcatgtaga cctcgatcag atccggcgtc cagcccgagc gcgcgacgtt cgcgtcgacg
     1021 gtgagcgcga cgtcccaacc gggccagccg gggccgctcc aacggagctc gcacaatcga
     1081 ccgatcgcct ccaggctgtg gaagcgcacg cggctcagct tggtcaggta ggtctcgcgg
     1141 tcgactagcg cgagcaatcg cacggggctg cctccgccat cggcttcgca tcaccgtagc
     1201 acagcccttc gcacgcgggg cccgctcggc tagactggca tgcggtggac cggctaccgg
     1261 tcgagcggcg ccggaggagc gaacgatggc gagcaatcgg tttctctcga ctcgaacggg
     1321 ttgggcgtcg tcgtgcgcgt tgctcgcgac gctcgccgtc tgcggttgcc gcggtacgac
     1381 ggcccccgac gagacggagc cggccgagga gttggcgatc gcggcggacg acgtgccgcg
     1441 ggaggtgcgc ctggcggccg gaatcgcgct cgacgcgctc gaagcgggcg ccgagtgtcg
     1501 cgccttcagc tgggatcgcg aagacgacat gtgggagtgc acggtcgacg ggctggcctt
     1561 tgccgcggag cccgatctcg accggaacgg cgccttctcc gagttcgagt tcgacgtcac
     1621 gctcgacgtg ctcgatcacc acgcgcccga gctcgcgagc ctgatccgcg acacctgcgg
     1681 ccgcgacgga gcggcgcacg tcgagacgtc gatccggcgg gtcgacctgc tcggacccga
     1741 cccgtccttc gacaggctgt ggggcgagga cgcgatcttc gtcgagttcc agtgtgccga
     1801 cgggacggac tacgaggtcg atgcgtacgg tgcgctcgtc atcgcacccg acgacgatgc
     1861 ggaagcgccg gccgtggccg atccgcgcgt cgaatagtcg ttgccgttcc gacggttcgg
     1921 gagcatactc agggtggaag ggggccgcat gagtccgccg aagagtccag ggccctcgag
     1981 tcgagcggct cgagggccac agcggggcga ggcttgacgg gtccatgagt cgccacgccg
     2041 tcggaacgag gccgcgaccg gtctagtcga ccaccagccg gcaagcttcg tcagcaaaaa
     2101 cggggagcgt cgctccccgt ttttctttgc cgggaccgat ctggcgcaac gcctcttgac
     2161 ggcggccggg taggcggcca tttatggcgg tcgagactcc ccgcctggac gagcgcgagg
     2221 cacgcatgaa gaagatccag cccgggttca tcctgccgag ctcggcattc acggtcagcc
     2281 cgcgactgat caagggctac gagaagttgg accgcccgcc gcgggtcggc gacgtcgtct
     2341 acggccgaat cagccatgtc ggccagcacg gatcgctcga gaaccgacag gggcgcatcc
     2401 acatgatcag cgacggcacg cgcgcggtct tcgtctacgg caaccgctac gcgccggacg
     2461 gcttcgaagg cgtcttgccg gagaccagcc tgccggaggt cgacctcttg gcacgctcgg
     2521 gactgatcgg cacgatgcga tcgaagaaca ccgcgatcaa ggacccgacc aaggttcgca
     2581 ttctgggaca cgcgatcggc gaggacctgc gcccgctgaa cacgatcgac cacccgatcg
     2641 cgctgcccaa gtccgacgtg atcgacacca agcggcgtgc gcggatgatc ctcaacgtcg
     2701 gaaccagcat gaactcgggc aagagcacga ccgcgatcgc ctgttgctgg gcgctgtcgt
     2761 cgatgggaca cgcggtgcgc gcggcgaagg tgaccggcac ggcaagcctg aaggacatcc
     2821 tgcacatgca ggacgccggc gcggagaaga tcgcggattt cacgcacctg ggttatccgt
     2881 cgacctacct gctcgagatc ggcacggtga tcgagatctt ccgtagcctc gacgccaagt
     2941 tcgccaacaa cccgaagaag ttctgggtcg tcgagttcgc cgacggcatc ctccagcgcg
     3001 aaacggcggc attgttgcgc gacgagtacg tgcgatcgag gatccaccgc ctgatcttct
     3061 cggcggtcga cgcgttcggt gcgctcggcg ggctgcgcgc gctgcaggag gaattcggtc
     3121 tgacccccga cgcggtttcg ggacgctgca cctcgtcgcc gctgatggtg cgggagctgc
     3181 agggacgcac gaacatcccg gtggtcaaca acgtcatccg tgacatgaag cagctgagcg
     3241 agatcctgct ttgagcgtcc cgacggcggt cgcggtccac ggcggggcgg gccgagtcgg
     3301 gcgtgaacga ttgaccggcc cggggcgccg ccggttcttc gacggcttgt ccgccgcgct
     3361 gtcggccgcg cacgagatcc tgcgacgcgg agggagcgcc gtacgggcgg tgacgaccgc
     3421 ggtccgcgtg ctggaggacg acgaggtgtt caacgccggt cggggcgccg cgttgtgtgc
     3481 cgacggctcg gtcgagctga gcgcatcggt gatgaacggg cggaacctgg cggtcggcgc
     3541 gatggtcggg cttcggcgca cgcgcaaccc ggtgctcggc gcggcggcgt tgctcgatca
     3601 caagcacggc ttgctgttcg gcgagcaggc ggacaactac gcgcgtgccg ccggtctcga
     3661 ccagatggag accgactact tcgtcacgcc ggtgcgggtc cgccagtggc aggcgctctg
     3721 cggctcgaag cgcgtcgtgc tcgaccacag tggcgacgag gaggcgcatg gcacggtcgg
     3781 tgccgtggca cgcgaccggc gcggcaacct ggcggccgcg acctcgaccg gcgggctggt
     3841 caaccagctg ccgggtcggg tcggtgactc gcccgtcgtc ggcgccggca cctgggccga
     3901 tcgcaggtgc gccatcagcg cgaccggcag cggggacgcc ttcgcccgca tcgccttcgc
     3961 tcggagggtc gccgacttcg tcgagctggc cgggatggag ccggccgcgg cggcgcggcg
     4021 cgcgctcgac gaggtggcac gggtccacgg ccgcggcgga tgcgtgctga tcggtgtcga
     4081 gggcgctccc gtcgccgtct tcaacagccc gcacatgatc cgcggctggg tcgtcgccgg
     4141 ggccgaggcg cgaatggcga tcgcgcccgg cgacgaggcc gcgcggcggc cgggcgatcg
     4201 ctgaagctca gccacccttg ccgccgtgct tgcgctcggg cgccggcagc cgtccggcct
     4261 cgcgcagcgc gcgccgcagc aggaagtcga gctgggcgtt ggcgctgcgc agctcgtcct
     4321 ccgcccagcg cctcagcgcg gccagcacct ccgggtcggt gcgtaacaga aaggaatcac
     4381 gcttggccaa cggcccggcc ccgtcgatca gtggtagagc gttccggtgt tcaccaccgg
     4441 ctgggtgtga cg
//
LOCUS       DASPGN010000232         8099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_625594, whole genome shotgun sequence.
ACCESSION   DASPGN010000232 DASPGN010000000
VERSION     DASPGN010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8099
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625594"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="VD788_06705"
     CDS             <1..1150
                     /locus_tag="VD788_06705"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXV75997.1"
                     /translation="VDELTAKLDAAEWEPLGSATAYRAPEQPLFRDSSELVLADGDAA
                     GGRRVNDLLNGGLHELMAADPDVMVLGEDLLDPYGGAFKVTKGLSTAYPDRVFSTPIS
                     EAAIVGFGNGWSLAGGKAVVEIMFGDFAALAVDQIVNQAAKMHFMYGGKVCVPVTVRL
                     VSGGYRGYGPTHSQSLESLFCGVPGLEVVALSRRHDPRALLGAAVGRDPNPVLFVENK
                     LIYSMRVHTGAPAGFRFVPGRAPSGSVYPSLRFTTTEPGAAADVTVVTYGGMTDMVEE
                     AMEQLILDEELEFDYFVLSQLSPLSVDDVVASVTVTKRLVTVEEGPRRYGVGTEVVSR
                     VAEALPGQSIRVALVGAEDLPIPNSRVQETDVLPSRERIVGAIRRVWS"
     gene            1151..2233
                     /locus_tag="VD788_06710"
     CDS             1151..2233
                     /locus_tag="VD788_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HXV75998.1"
                     /translation="MKSKHEWILVPRVAANDDHALLVGWVKRDGERVAKDDVICELEF
                     SKSVVELPASRDGWLFHLRSVGDEVSVGTRLAAISSANERPAPAAHHDLSSIKVTAKA
                     RALIETHGLDIGVFEGLDIVKEKHVRALLDTREPALEEVAEGELLALTPVRRRAAQAV
                     SESKQTIPHSHLCRWVSADRVEASVGRLARKHDMMVSVADWLVATVVHEAARNRKVNA
                     SWRSSGIFVHSKLNVGYALNQPNGELLVPVVVDADRLGLEDLVGRLRGLQKKALRHRL
                     TPAELSGGTITVTSLVGSGVHQVFPILVPGQAAIVALGDRAALGGAATYGLTVGFDHR
                     VLNGTEAAEFLTAVAVRLEGAEQDTP"
     gene            2230..2472
                     /locus_tag="VD788_06715"
     CDS             2230..2472
                     /locus_tag="VD788_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXV75999.1"
                     /translation="MSTKETVIGQIAAVLQLDEAEIENLRKDVGYKRLKKWTSARHVE
                     IIVAVEDVFDIEVDERSIPKLNDVAKIVAYVEAARS"
     gene            2469..2840
                     /locus_tag="VD788_06720"
     CDS             2469..2840
                     /locus_tag="VD788_06720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HXV76000.1"
                     /translation="MSRQAAPLGVGIDISHERDFVDLIDNRVALERTFSAREIDECLA
                     AGQDAAAALARRFAAKEALMKACRHVTGALDRIEIVHDDDGAPLARWEHLRANGLRAQ
                     ISLSSAHSVAVAVAIVTAAGE"
     gene            2993..5836
                     /locus_tag="VD788_06725"
     CDS             2993..5836
                     /locus_tag="VD788_06725"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXV76001.1"
                     /translation="MTRADESARLPAAAKWVMLIYFLSGVCSLMDEVVWVRLLKLTLG
                     NTVYATTVVVSVFMGGLALGAWCMSRYADRIARRLHWYAVLETIATASALAVPWLLAL
                     ADRVYVWFFRAHHPAHGTLLFVQVLLSAAIILVPSITMGSTLPLLARFVTGLERESGR
                     LVGRLYALNTMGAACGCFLAGFLLIRAIGVMGTLSAAAALHLVVAAAGWRLSRRRIDE
                     TEPEPVAPSGVAAPAASFPVSFPLLAAAFFLSGFVSIGYEIGWFRSVVFLLGGDTYVF
                     TSVLTVYLLGNVVGAAIGSRLVPRLANPAAAFAVTLALLGLSGALYFPLLVVWTERWA
                     APLGRGMTWVAAHSPLAAALVKPLGHSLGLFLLPSALMGVGFPIALQAWAARVHRVGR
                     STGAAYAANTIGAVLGGVVTGFVLIPALGMQRSVMLLGLVLVLVAAALYLTAQPRPLP
                     RRRWLVALTPVVLVTLAMLTTADRLLALIEASPWLKDELQTVSVREGVTATVTVHRDE
                     TQQTLHLYSSGHSIAGDTFALRGDQKALGHFGVLLNPTAREVLSVGFGSGETTKCLSD
                     HGLDRIDCVEVAPEVVRSSLEYFRHLNLADRLDEEVELIFMDAKNFVHLTDRSYDVVV
                     NDSIHPRDFAENASLYGKEYFEAIRRRLNPGGLMVSWLPTYHMSARDFDSILGTLMDA
                     FPHVSLWYLIQNPAPLVVLVASEGPQRYAPAAIDDAIAQPAIGESLAVIGIHDAIDLL
                     NCYMGDERELRPIVRDYAVNSDYTPFIEFSTEEWTPADEMYQRFVLDIRGESIFDHLD
                     LSGFDDAEAAAWLAEFRGRHRATGLLAQSLVKTNELERLKLITDALGLAPGDLALLRA
                     LDGSERTLVGKGQTILETEGADRALGLADVILAIHPESSSAWLIRSTVMHRRGDLPRA
                     LFAARKAVEMAPDSDEARDHLAELTAELRSAGG"
     gene            complement(5853..6140)
                     /locus_tag="VD788_06730"
     CDS             complement(5853..6140)
                     /locus_tag="VD788_06730"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HXV76002.1"
                     /translation="MTAASGKRDELIAILLEGLKDMPGCLTYVVAKDAVDADPIWVTE
                     VRDSKESHQASLSLPAVRRAIERGRPLIGGFGQSVETDPVGGHGLTEVGEP"
     gene            6304..6843
                     /locus_tag="VD788_06735"
     CDS             6304..6843
                     /locus_tag="VD788_06735"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXV76003.1"
                     /translation="MTNDRILNRKLLPANTCFGCGHENPHGLHIEVLHDPADAAVLRG
                     RFVPHAGMTGFPGVVHGGVIFSALDCLATWVVSLLRDEPDALWLLRSAETRYHKPALA
                     ERELALVGRIADESGAWRPVVVHAEARDDRGDLVSECDFKEVPLSIDKFERISGLAPL
                     PENWRTFLANVRRPRFPPG"
     gene            7045..7410
                     /locus_tag="VD788_06740"
     CDS             7045..7410
                     /locus_tag="VD788_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76004.1"
                     /translation="MVLSKNQIATGAVLLLLFASPLGSSLAAAENKKGGLKEAGRELE
                     EIGRKVGEAGKEAGREIAGAAKHVFYKGKKASAPLLRDVQRSTREFWREVIAEQDKTI
                     EQLRLENDELKRRLGELDG"
     gene            7407..7670
                     /locus_tag="VD788_06745"
     CDS             7407..7670
                     /locus_tag="VD788_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76005.1"
                     /translation="MSRRITTVTVLLTLLVLHAACGEQPSPAADPYIERAADREEREA
                     LTLARDDIDREQRAVVAQKDAEIERLREEQEELRAKLAARAGN"
     gene            7702..>8099
                     /locus_tag="VD788_06750"
     CDS             7702..>8099
                     /locus_tag="VD788_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76006.1"
                     /translation="MPKNKALLIVDEIARNAAMAVPAVSRWRARRGRTSVQTDRIDSA
                     YLRRYAHLPFDTVTEHVGPSGIERLHVGEIGPGDHIPAALLFLGAGAASYTCYDRFAG
                     DVAGERAKSIYLALVEELRRDRPELARRLE"
BASE COUNT         1213 a         2734 c         2804 g         1348 t
ORIGIN      
        1 ggtcgacgag ctcaccgcga agctcgacgc ggccgagtgg gaaccgctgg gttccgcgac
       61 ggcctatcga gcgccggaac aaccgttgtt ccgagactcg tcggagctgg tgctcgccga
      121 cggggacgcg gcggggggcc gcagggtcaa cgacctgctc aacggggggt tgcacgagct
      181 gatggccgcc gatcccgacg tgatggtgct cggcgaggat ctgctcgatc cgtacggcgg
      241 cgcgttcaag gtcaccaagg gtctgtcgac ggcctacccc gaccgggtct tctcgacacc
      301 gatatcggaa gccgcgatcg tcggcttcgg caacggatgg tcgctcgccg gcggcaaagc
      361 ggtggtcgag atcatgttcg gcgacttcgc ggcgctcgcg gtcgatcaga tcgtcaacca
      421 ggcggcgaag atgcacttca tgtacggcgg gaaagtctgc gttccggtga ccgtgcggct
      481 ggtctcgggc ggctatcgcg ggtacggtcc gacccacagt cagagtctcg agagcctgtt
      541 ctgcggagtg cccggtctcg aggtcgtcgc gctgtcccgc cggcacgacc cgcgcgcgct
      601 gctcggtgcc gcggtcggac gcgacccgaa cccggtgctg ttcgtcgaga acaagctgat
      661 ctactcgatg cgcgtccaca ccggcgcgcc ggccggcttc cgcttcgtgc ccggccgcgc
      721 gccgtccggc tcggtttacc cgtcgctgcg attcaccacg accgagcccg gggcggcggc
      781 ggacgtgacc gtcgtcacct acggcggcat gacggacatg gtggaggaag cgatggagca
      841 actgatcctc gacgaggagc tggaattcga ctacttcgtc ctgtcgcagc tcagcccgct
      901 gtcggtcgac gacgtcgtgg cctcggtcac cgtgaccaag cgcctggtga ccgtcgagga
      961 gggcccgcgg cgttacggag tcggcaccga ggtcgtttct cgcgtcgcgg aggcgttgcc
     1021 ggggcagtcg atccgggtcg cactggtcgg cgcggaggac ctgccgattc ccaactcgcg
     1081 cgtgcaggag accgacgtgc taccgtcgcg cgagcggatc gtcggagcga tccgtcgggt
     1141 gtggtcgtaa gtgaagagca aacacgagtg gatcctggtg ccgagggtcg cggccaacga
     1201 cgatcatgcc ctgctcgtcg gctgggtcaa gcgcgacggc gagcgggtgg ccaaggacga
     1261 cgtgatctgc gagctcgagt tcagcaagtc ggtcgtcgag ctgccggcct cgcgcgacgg
     1321 ttggctgttc cacctgcgga gcgtggggga cgaggtgtcg gtcgggacgc ggttggccgc
     1381 gatctcctcc gcgaacgagc gacccgcgcc ggccgcgcac cacgacttgt cgagcatcaa
     1441 ggtcacggcg aaggcccgcg cgctgatcga gacgcacggg ctcgacatcg gcgtgttcga
     1501 gggactggac atcgtcaagg agaagcacgt ccgggcgctg ctcgacacgc gggaacccgc
     1561 gctcgaggag gtggctgaag gcgagctcct ggcgttgacg ccggtgcggc gccgggccgc
     1621 ccaggcggtg agcgagtcga agcagacgat cccgcactcc catctctgcc gctgggtctc
     1681 ggccgaccgg gtggaggcgt cggtcgggcg attggccagg aagcacgaca tgatggtcag
     1741 cgtggccgac tggctggtgg ccaccgtcgt gcacgaggcc gcgcgcaatc gcaaggtcaa
     1801 cgcctcgtgg cgctcgagcg ggatcttcgt tcattccaag ctgaacgtcg gctatgcgct
     1861 gaaccagccg aacggcgagt tgctcgtccc ggtcgtggtc gatgccgatc gtctggggct
     1921 cgaggatctg gtgggccggc tgcgcgggct gcaaaagaaa gcgctgcgcc accggctgac
     1981 tccggcggag ctgtccggcg ggacgatcac ggtcaccagt ctggtcggca gcggcgtgca
     2041 ccaggtcttt ccgattctgg ttccggggca ggccgcgatc gtcgcgctcg gcgatcgtgc
     2101 cgcgctcggc ggcgccgcga cctacggtct caccgtcgga ttcgatcacc gcgtgctcaa
     2161 cggcacggag gcggccgagt tcctcaccgc ggtcgccgtg cggctcgagg gtgcggagca
     2221 ggacacacca tgagcaccaa ggaaaccgtg atcgggcaga tcgccgccgt gctgcagctc
     2281 gacgaggcgg agatcgagaa cctgcgcaag gacgtcggct acaagcgcct caagaaatgg
     2341 acctccgcgc ggcacgtcga gatcattgtc gccgtcgagg acgtcttcga catcgaggtc
     2401 gacgagcgat cgatcccgaa gctcaacgac gtggccaaga tcgtggccta cgtcgaagcc
     2461 gcccgctcgt gagtcggcag gcggcgccgc tcggcgtcgg aatcgacatc tcgcacgaac
     2521 gcgacttcgt cgacctgatc gacaaccgcg tcgcgctcga acggaccttt tccgctcgcg
     2581 agatcgatga atgcctggcc gccgggcaag acgccgcggc ggctctcgcc cgtcgtttcg
     2641 ccgccaagga agcgctgatg aaggcctgtc gccacgtgac gggcgcgctc gaccggatcg
     2701 agatcgtcca tgacgacgac ggtgcgcccc tcgcgcgctg ggagcatctc cgcgccaacg
     2761 gtctgcgggc gcagatcagc ctcagctccg cgcactcggt ggcggttgcg gttgcgatcg
     2821 tgacggcggc cggcgagtag cggccgcgga tgccgctcag ctcgctgacc gtgatcggcg
     2881 gctcccaccg cgccgagacg ctcggatcgc gctgtcggcg aggccgctgg tatcattccc
     2941 ggaccgtccg gctcgtcgat cgggccgacg acgcgaggtt caccgacccc cgatgacgcg
     3001 agccgacgag tccgcgagac tgcccgccgc ggcgaagtgg gtcatgctga tctacttcct
     3061 gtccggtgtc tgctcgctga tggacgaggt tgtctgggtc cggctgctca agctgacgct
     3121 ggggaacacg gtctacgcca cgaccgtcgt cgtctcggtc ttcatgggtg ggctcgcgct
     3181 cggcgcttgg tgcatgtcgc gctacgccga tcggatcgcg cggcggctcc actggtatgc
     3241 ggtgctcgag acgatcgcga cggcctccgc gctggccgtg ccgtggttgt tggcgctcgc
     3301 cgaccgcgtc tacgtctggt tctttcgcgc ccaccatccg gcacacggca cgctgttgtt
     3361 cgtccaagtg ctgctatcgg ccgcgatcat cctcgtgccg tcgatcacga tgggcagcac
     3421 gctgccgttg ctcgcgcgct tcgtcaccgg gctcgagcgc gagtcgggca ggctcgtcgg
     3481 ccggctctat gcgctgaaca cgatgggcgc cgcttgcggg tgcttcctcg ccgggttcct
     3541 gctgattcga gcgatcggcg tgatggggac gctctccgcc gccgcggcgt tgcacctggt
     3601 cgtcgccgcg gcgggttggc ggctcagccg ccggcgcatc gacgagaccg agcccgagcc
     3661 ggtcgcgccg agcggggtcg ccgcgccggc cgcctcgttc cccgtctcct tcccgctcct
     3721 ggccgcggcg ttcttcctca gcggcttcgt cagcatcggc tacgagatcg gctggttccg
     3781 cagcgtcgtc ttcctgctcg gcggcgacac ctatgtcttc acttcggtgc tgacggtcta
     3841 tctgctgggc aacgtcgtcg gcgcggcgat cggcagccgg ttggtaccgc ggctggccaa
     3901 ccccgcggcc gcgttcgcgg tgacgctggc actgctcggc ctgtcgggcg cgctctactt
     3961 cccgctgctc gtcgtgtgga ccgagcggtg ggccgcgcca ctcggtcgtg ggatgacctg
     4021 ggttgccgcg cactcgccgc tggccgccgc gctggtcaag ccgctcggtc acagcctcgg
     4081 tctgttcctg ctgccgtccg cgttgatggg tgtcggcttc ccgatcgcgc tgcaggcgtg
     4141 ggccgcgcgc gtgcatcgcg tcggacgctc gaccggcgcc gcatacgccg ccaacacgat
     4201 cggcgccgtc ctcggtggag tcgtcaccgg gttcgtgctg atcccggcgc tcggcatgca
     4261 gcgctcggtg atgttgctcg gcctggtgct ggtgctcgtc gccgcggcgc tgtacctgac
     4321 ggcccaaccg cggccgctgc cgcggaggcg ttggctggtc gcgttgaccc cggtcgtgct
     4381 ggtgactctc gcgatgctga ccaccgcgga tcgactgctc gcgctgatcg aggccagccc
     4441 gtggctcaag gacgagctgc agaccgtctc ggtgcgcgag ggcgtgacgg caacggtgac
     4501 ggttcaccgg gacgagacac aacagacgct tcatctctac tcgtcggggc acagcatcgc
     4561 cggcgacacc ttcgcgctac gcggcgacca gaaggcgctc ggtcacttcg gcgtgctgct
     4621 caatccgacg gcgcgcgagg tgttgtcggt cgggttcggc tccggcgaga cgaccaagtg
     4681 cttgtcggac cacggtctcg accgcatcga ctgcgtcgag gtcgcccccg aagtcgtgcg
     4741 ctcgtcgctc gaatacttcc gccacctcaa tctcgccgat cgactggacg aggaagtcga
     4801 gctgatcttc atggatgcca agaacttcgt gcacctgacc gatcgcagct acgacgtcgt
     4861 cgtcaacgac tcgatccacc cgcgcgactt cgccgagaac gcgtcgctgt acggcaagga
     4921 gtacttcgag gcgattcgcc ggcggctcaa ccccggcgga ttgatggtgt cgtggctgcc
     4981 gacttaccac atgtccgccc gtgatttcga cagcatcctc gggacgttga tggacgcctt
     5041 cccccacgtc tcgctgtggt acctgatcca gaacccggcc ccgctggtcg tgttggtggc
     5101 atcggagggc ccgcagcgtt acgcgcccgc cgcgatcgac gacgcgatcg cccagcccgc
     5161 gatcggcgaa agcctcgcgg tgatcgggat ccacgacgcg atcgacttgt tgaactgcta
     5221 catgggggac gagcgcgagc tgcgtccgat cgtgcgagac tacgccgtca acagcgacta
     5281 cacgccgttc atcgagttca gcaccgagga atggaccccg gccgacgaga tgtaccaacg
     5341 tttcgtcctc gacatccgcg gcgagtcgat cttcgaccat ctcgacctgt cgggcttcga
     5401 cgacgcggag gccgccgcct ggctcgccga gtttcgcggc cggcaccgcg cgacgggcct
     5461 gctggcacag tcgctggtca agaccaacga gctcgaacgg ctgaagctga tcaccgacgc
     5521 gctgggcctc gcgccgggcg atctggccct gctgcgtgcg ctcgacggct ccgagcgcac
     5581 actcgtcggc aagggccaga cgattctcga gaccgagggg gccgaccgtg ccctcggtct
     5641 ggccgacgtg atcctcgcga tccatcccga gtcgtcgtcc gcgtggttga tccgctcgac
     5701 cgtcatgcac cggcgggggg acctaccgcg cgcgctgttc gccgcgcgga aagcggtcga
     5761 gatggccccc gacagcgacg aggcccgcga ccacctcgcc gagttgacgg ccgagctgcg
     5821 cagcgcgggc ggctagccgc ggcgcgagac tgtcacggct cccccacttc cgtcagaccg
     5881 tggcccccga cgggatcggt ctcgacgctc tgtccgaagc ccccgatcaa cggccggccg
     5941 cgctcgatcg cccggcgaac cgccggaagc gagagggagg cctggtggct ctccttgctg
     6001 tcccggacct cggtgaccca gatcggatcc gcgtcgaccg cgtccttggc cacgacatag
     6061 gtcaggcagc cgggcatgtc cttcagtcct tcgagcagga tcgcgatcaa ctcgtcgcgc
     6121 tttccgctcg ccgccgtcat ctgaccatcc ccagtgctct tcccatcacg acagccatct
     6181 tccttgtgct cccactcgac gtttcgtcga ccgccgtacc cctcaacgta taccccactc
     6241 gcccgttcac gtgtagagtg ctctcgggcc ttcgatcgat cgaccggcga ggaggattgg
     6301 cgtttgacca acgaccggat cctgaaccgg aagctcctgc ccgcaaacac ctgcttcggt
     6361 tgcgggcacg agaaccccca cggcctgcat atcgaggtgc tgcacgaccc cgccgacgcc
     6421 gcggttcttc gtggccggtt cgtgccccac gcgggcatga cgggattccc cggtgtggtc
     6481 cacggtgggg tgatcttctc ggcgctcgac tgtctcgcca cgtgggtggt cagcctgctg
     6541 cgggacgagc ccgatgcgct gtggctgttg cgatccgcgg agaccaggta ccacaagccg
     6601 gcgctggcgg agcgggagct cgcgctcgtc gggcgcatcg ccgacgagag cggcgcgtgg
     6661 cgaccggtcg tcgtgcacgc cgaggcacgc gacgaccgcg gcgatctcgt cagcgagtgc
     6721 gacttcaaag aggttccgct gtccatcgac aagttcgagc gcatcagcgg ccttgccccg
     6781 ctccccgaga actggcgaac cttcttggcc aacgtgagac gaccgcgctt cccaccagga
     6841 tagacgttcc taccggcctg gccggcgccg gggtttctgc ggtggcagcc cgtataccaa
     6901 actcgcacag acgagcgaca aggggcggta gcacgtgatt cgaagggacg tcggcgctgc
     6961 gatcgcaacg gcttcgccgt gacaggtggc cgtctccgcc ctaagttcga ggagaagtga
     7021 cccgggggga gcgaggtttc gccgatggtc ctgtcgaaga accagatcgc gactggagcg
     7081 gttcttcttc tgctcttcgc ctcgccgctc ggctcctcgc tcgctgccgc ggaaaacaag
     7141 aagggcggct tgaaggaggc cggccgcgag ctcgaggaaa tcggccgcaa ggtcggagaa
     7201 gcgggaaagg aggcgggccg cgagatcgcg ggtgccgcga agcacgtctt ctacaagggg
     7261 aagaaggcct cggcgccgct gctgcgcgac gtccagcgtt cgacgcgtga gttctggcga
     7321 gaggtcatcg cggagcagga caagacgatc gagcagctcc gcctcgagaa cgacgagctg
     7381 aagcgacgcc tcggggagct cgacgggtga gccggcgcat cacgaccgtg acggtgctgc
     7441 tgacactcct cgtgctccac gcggcgtgcg gcgagcagcc gtctccggcc gcagacccgt
     7501 acattgagcg cgcggccgac cgcgaggagc gagaggcact gacgctcgcg cgcgacgaca
     7561 tcgaccgaga gcagcgtgcg gtcgtcgccc aaaaagacgc cgagatcgag cgcctccgcg
     7621 aggagcagga ggagctcagg gccaagctcg ccgctcgcgc tggcaactga ccgggctatc
     7681 ctggtgccgg aggagacggg cttgcccaag aacaaggcct tgttgatcgt cgacgagatc
     7741 gcacgcaatg cggcgatggc ggtcccggcc gtttcgcgct ggcgcgctcg acggggccgc
     7801 acgtcggtgc aaacggaccg gatcgactcc gcctatcttc ggcgctatgc ccacctcccg
     7861 ttcgacacgg tcaccgagca cgtggggccc tcgggaatcg aacggttgca cgtgggagaa
     7921 atcggaccgg gcgatcacat tccggcggca ctgctgttcc tcggcgctgg cgcggcgagc
     7981 tacacgtgct acgaccgctt cgccggcgac gtcgccggcg agcgggccaa gtcgatctac
     8041 ctcgcgctgg tcgaggagtt gcgacgcgac cggcccgagc tggcgcggcg gctcgagga
//
LOCUS       DASPGN010000233         6097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_509004, whole genome shotgun sequence.
ACCESSION   DASPGN010000233 DASPGN010000000
VERSION     DASPGN010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509004"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VD788_06755"
     CDS             complement(<1..637)
                     /locus_tag="VD788_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXV76007.1"
                     /translation="MLDVLRRSLPAGLAAGWVLVAVAPAAAQEVVIAGSESESPSVFF
                     VDSRGSSPYLGVQLEEETDWHEGGARVTAVVGDSPAERAGLREGDIIVEFGGKTIRGP
                     VALTNAIHEKEAGDTVELKIVRDGKKQSLDAELGERGAAVGYSYAPGALEWVAPEIDE
                     ELREKLDRSLEHVYGLGSCQSEECTSFGFSFNWAGRPRLGVQLVDATPELRQ"
     gene            833..1195
                     /locus_tag="VD788_06760"
     CDS             833..1195
                     /locus_tag="VD788_06760"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXV76008.1"
                     /translation="MTTDTRFRQRDKRYPVLRPAAAFIEFHFGRRPWKLPLLDLSVGG
                     LSFRTTDEAPGVAAGARLESVLHVGDCQMRGVVIVRHATEQTDTTVHYGGVFYPAEEG
                     DLLKLNGVLGGIDTISPR"
     gene            complement(1205..1861)
                     /gene="rpiA"
                     /locus_tag="VD788_06765"
     CDS             complement(1205..1861)
                     /gene="rpiA"
                     /locus_tag="VD788_06765"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HXV76009.1"
                     /translation="MSRDELKIHAARAALDEIPDGAVIGVGTGSTTDHFIDGLAAIAA
                     RLRGAVASSVATERRLRSAGIDLFDLNQVGGVALYVDGADEATRARRLIKGGGGALTR
                     EKILAAASERFVCIIDRHKLVERLGAFPLPVECVPMARGLVARELARLGGRAVWREGF
                     TTDNGNVILDTHGLSIDDPDALECELNQLPGIVTVGLFCRRPADRLIVGEPDGVRYID
                     "
     gene            complement(1858..4842)
                     /locus_tag="VD788_06770"
     CDS             complement(1858..4842)
                     /locus_tag="VD788_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HXV76010.1"
                     /translation="MSVPHNPRGAERPQRRLRRFQDLMRHRVQHILLVASLYDSFILT
                     EDGHLNEALLRQFIDLNLSHNPDLIRVSSGADALAMIAEERRFDMIITSMQLGDMNAV
                     ELARAARRAGIDLPVIMLAYNNRELTDFLARNDVSAIDRVYLFQGDVRIMLAMVKDVE
                     DRWNLAHDTGVIGVPAIIVVEDNVRFYSSFLPVIYTELMNHVHNLISEGLNLSQKLLR
                     MRARPKVLLCDNFEQAWDYFSAYDDRILGIISDIEFPVGGRLDRGAGVELATRVRRVR
                     PDIPIVLQSSIPANQELARELGAYFLLKGSPLLLHQLREVLVDRFGFGDFVFRLPDGS
                     EIDRANDLRTLIEKLRTVPAESIAYHGERNHFSNWLKARTEFALAEKLRPRKVSDFDS
                     VEKLRQDLVRSIDEYRFDRDKAIVADFDRNDPEVSSSISRIGGGSLGGKARGLAFANR
                     LLVEANVQAHFPEVRITVPTAVVLATDIFDQFVERNDLEDFAIGSTDDAEIERRFLSA
                     PFPPEPLADLRAFLARVDYPLAVRSSGLYEDAPSQPFAGVYETFWVTNLDDRLDDRLL
                     GLVEAVKRVYASTFSQRAKAFLRMTPFRLEEEKMGVIIQRIVGRARGGRFYPDFSGVA
                     RSHNFYPTPPQRAEDGIAAVALGFGRAVVDGTTCVRFCPRYPRHLVAFSSVDDVLDNS
                     QREFFALDLTNPDGKRTRQDDELSSYGLDVADRDGTLAAVGSTYSPENDVVYDGTSRR
                     GVRLVSFAPILKHDVFPLAELLDVMLDYGAEGTGTPVEIEFAVSLDHADQRPPEFGFL
                     QMRPLSPMSEVEDLEIGEVMSSALVCRSASVLGHGRVADLRDLVVVDAHRFDRMRSHE
                     AAQQVAQINAALLAEELSYLLIGVGRWGSSDPFLGIPVSWAQIAGARVIVESGFKDFR
                     VTPSQGTHFFQNLTSCNVGYFTVNPEAGDGFIDWGWLAAQPATRETQFVRHIRLDGPV
                     TVKMSGRTGQGVILKPDGGDGR"
     gene            complement(4937..>6097)
                     /gene="gdhA"
                     /locus_tag="VD788_06775"
     CDS             complement(4937..>6097)
                     /gene="gdhA"
                     /locus_tag="VD788_06775"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HXV76011.1"
                     /translation="DQGNVQVNRGYRIEMNSAIGPYKGGLRFHPSVNLGILKFLAFEQ
                     VFKNSLTTLPMGGGKGGSDFDPKGKSDQEVMRFCQSFMTELFRHIGQNTDVPAGDIGV
                     GGREIGFLFGQYKRLANEFSGVLTGKGLNWGGSLIRPEATGYGAVYFAAEMLATRKQT
                     LEGKRCLVSGSGNVAQYTTEKLLQLGAKVLTLSDSGGYIYDEAGITTDKLEFVINLKN
                     VRRGRIKEYADKFSSATYTAADAGGDYNPLWRHKADCAFPSATQNEINGKDAQNLLNN
                     GCYLISEGANMPTTADGVSLFVEARVLYGPGKAANAGGVAVSGLEMAQNSMRYSWTRD
                     EVDNRLQLIMKSIHESCVAAADRFGTPGNYVNGANIAGFLKVADSMLDQGVV"
BASE COUNT          989 a         2088 c         2019 g         1001 t
ORIGIN      
        1 gttggcggag ctccggcgtg gcgtcgacga gctgcacgcc gagacggggc cgaccggccc
       61 agttgaaact gaaaccgaac gaagtacatt cctcgctctg gcagctaccc agaccgtaga
      121 cgtgctcgag acttcgatcg agcttctcgc gcagctcctc gtcgatctcg ggcgcgaccc
      181 actcgagtgc gccgggcgcg tacgagtaac cgaccgccgc accgcgctcg cccaactccg
      241 cgtcgagcga ctgcttcttc ccgtcacgca cgatcttcag ctcgacggtg tcgcccgctt
      301 ccttctcgtg gatcgcgttg gtcagcgcga ccggcccgcg aatcgtcttg ccgccgaact
      361 cgacgatgat gtcgccctcg cgcagaccgg cccgctcggc cggcgagtcg ccgaccaccg
      421 ccgtcacgcg cgcgccgccc tcgtgccagt cggtctcttc ctcgagttgg acgccgaggt
      481 agggcgacga gccccgcgaa tcgacgaaga aaacggacgg gctctcgctc tccgatccgg
      541 cgatcaccac ttcctgtgcc gcggccggag caacggcgac caggacccaa cccgcggcca
      601 ggccggccgg taacgatcgt ctcagcacgt cgagcatcgc tcgcttcctc ctgcatgctg
      661 catgcggccc accggataat acgcccggcg gcaccgaaag tcggggaacc cggtgcggtc
      721 ggacggcgcc cgaagctcgc cggccgcccg agctagcgtc cggggcgctc cggcctatat
      781 tcggccgtgg agcgtccgcc gcggaacctg cggcgagggc ggaggatcgg ccatgacgac
      841 cgacaccagg tttcggcagc gggacaagcg ttaccccgtc ctgcggccgg cggcggcctt
      901 catcgagttc cacttcgggc ggcgaccgtg gaagctgccc ctgctcgatc tcagtgtcgg
      961 tggtctgtcc ttccgcacca ccgacgaggc gccgggggtc gccgccggag cgcgcctcga
     1021 atcggtgctc cacgtcggtg actgccagat gcgcggagtc gtcatcgttc gtcatgcgac
     1081 cgaacagacc gacacgacgg tgcactacgg cggcgtgttc tatcccgccg aagaaggtga
     1141 cctgctcaag ctcaacggag tgctgggcgg aatcgacacg atcagcccgc gttgaactgc
     1201 ccgctcagtc gatgtatcga acgccgtccg gttcgccgac gatcagccgg tccgccgggc
     1261 ggcggcagaa cagaccgacg gtgacgatgc ccgggagctg attcagctcg cactcgagcg
     1321 cgtccggatc gtcgatcgac agcccgtgcg tgtcgaggat cacgttgccg ttgtcggtcg
     1381 tgaacccctc gcgccagacc gcacgcccgc ccagcctggc cagctcccgt gcgacgagcc
     1441 cgcgtgccat cggaacgcac tcgaccggca gcggaaacgc cccgagccgt tcgacgagct
     1501 tgtgtcggtc gatgatacag acgaaacgct cgctcgcggc ggcgaggatc ttctcgcgag
     1561 tcaacgctcc tcccccgccc ttgatcaatc gccgagcgcg cgtcgcctcg tcggctccgt
     1621 cgacgtacag cgccacgccc ccgacctggt tgaggtcgaa cagatcgatt cccgccgagc
     1681 gcagccggcg ctcggtcgcc accgagctgg cgacggcacc gcgcagccgc gcggcgatcg
     1741 cggccaagcc gtcgatgaag tggtcggtcg tcgagccggt gccgacgccg atcacggcgc
     1801 cgtcggggat ctcgtccagc gcggcgcgcg cggcgtggat cttcagctcg tctcggctca
     1861 tcgaccgtcg cctccgtcgg gcttgaggat cacgccctgg ccggtgcggc cgctcatttt
     1921 gacggtcacc ggaccgtcga gacggatgtg gcggacgaac tgcgtctcgc gcgtcgccgg
     1981 ttgggccgcg agccagcccc agtcgatgaa cccgtcgccg gcctcggggt tgaccgtgaa
     2041 gtagccgacg ttgcacgagg tcagattctg gaagaaatgg gtcccctggg atggggtgac
     2101 gcggaagtcc ttgaagccgg attcgacgat cacgcgggcc ccggcgatct gcgcccagct
     2161 gaccgggatg ccgaggaacg gatcactcga cccccagcga ccgacgccga tcagcagata
     2221 cgacagctcc tcggcgagca gcgccgcgtt gatctgcgcc acctgctgcg ccgcttcatg
     2281 gctccgcatg cgatcgaagc gatgggcgtc gacgaccacc aggtcccgca gatccgccac
     2341 ccggccgtgg cccagcacgc tcgcgctgcg gcagaccagc gcggaggaca tcacctcgcc
     2401 gatctcgaga tcctcgacct cgctcatcgg cgacaacggg cgcatctgca ggaagccgaa
     2461 ctcgggcggc cgctggtcgg cgtggtcgag gctgaccgcg aactcgatct cgaccggtgt
     2521 ccccgtaccc tcggcgccgt agtccagcat cacgtcgagc aactcggcga gcgggaagac
     2581 gtcgtgcttg aggatcgggg cgaagctgac caggcggacg ccgcggcgcg aagtgccgtc
     2641 gtagaccacg tcgttctccg gcgaataggt cgacccgacg gcggccagcg tcccgtcccg
     2701 atcggccacg tcgagcccgt aggacgacag ctcgtcgtcc tgtctcgtgc gcttgccgtc
     2761 ggggttggtc aggtcgagcg cgaagaactc gcgttgcgag ttgtccagca cgtcatcgac
     2821 cgaggaaaac gccaccaggt ggcgtgggta gcgcgggcag aagcgcacgc acgtcgtgcc
     2881 gtcgacgacc gcccggccga agccgagcgc gactgcggcg atcccgtcct cggcgcgctg
     2941 cggcggcgtc gggtagaagt tgtgcgagcg ggcgacgccg gagaagtcgg ggtagaagcg
     3001 tccgccgcgc gcgcgcccga cgatccgctg gatgatcacc cccatcttct cttcctcgag
     3061 gcggaacggc gtcatccgca ggaaggcctt ggcgcgttgc gagaacgtcg aggcgtagac
     3121 ccgcttgacc gcctcgacca gcccgagcag ccgatcgtcc aaccgatcat cgaggttggt
     3181 gacccagaag gtctcgtaga cgccggcgaa cggctggctc ggtgcgtcct cgtacaatcc
     3241 cgacgagcgc acggccagcg ggtagtcgac gcgcgccaag aacgcgcgca agtcggcgag
     3301 cggctcgggg gggaatggcg cggagaggaa ccggcgttcg atctccgcgt cgtcggtcga
     3361 accgatggcg aaatcctcca gatcgttccg ctcgacgaac tggtcgaaga tgtcggtggc
     3421 gagcacgacc gccgtcggta cggtgatccg cacctcgggg aaatgagcct gcacgttggc
     3481 ctcgaccagc agtcgattgg cgaacgccag accgcgcgcc tttccgccga gcgagccacc
     3541 gccgatgcgc gagatgctgc tcgacacctc ggggtcgttt cgatcgaagt cggcgacgat
     3601 cgccttgtcg cgatcgaacc ggtactcgtc gatcgaacgc accaggtcct ggcgcagctt
     3661 ctcgacgctg tcgaaatccg agaccttgcg tggccggagc ttttcggcca aggcgaactc
     3721 ggttcgcgcc ttcaaccagt tggagaagtg gttgcgctct ccgtggtatg cgatcgactc
     3781 ggccggaacc gtgcgcagct tctcgatcag cgtgcgcaag tcgttggcgc gatcgatctc
     3841 gctgccgtcg ggcagccgga agacgaaatc gccgaaaccg aaccgatcga ccagcacctc
     3901 gcgcagctga tgcagcagca gcggcgaccc cttgagcagg aagtacgcac ccagctcgcg
     3961 cgccagctcc tggttggccg ggatgctcga ctgcagcacg atcgggatgt cgggccgcac
     4021 gcggcggacc cgcgtcgcca gctcgacgcc ggcgccgcgg tcgagtcgcc cgccgacggg
     4081 gaactcgatg tccgagatga tgccgaggat ccggtcgtcg taggccgaga agtagtccca
     4141 cgcctgctcg aagttgtcgc acaacagcac cttcggccgc gcgcgcatgc gcaacagctt
     4201 ttgcgacagg ttgagcccct ccgagatcag gttgtggacg tgattcatca gctcggtgta
     4261 gatcaccggc aggaacgagg agtagaagcg gacgttgtcc tcgacgacga tgatcgccgg
     4321 cacgccgatc acaccggtgt cgtgcgccag gttccagcgg tcttcgacgt ccttgaccat
     4381 cgccagcatg atccgcacgt cgccctggaa cagatagacg cggtcgatcg ccgagacgtc
     4441 gttgcgggcg aggaagtcgg tcagctcgcg gttgttgtag gccagcatga tcaccggcag
     4501 gtcgattccg gcccggcgcg ccgcgcgcgc cagctcgacg gcgttcatgt cgcccaactg
     4561 catgctggtg atgatcatgt cgaaccgccg ctcctcggcg atcatcgcca gcgcgtcggc
     4621 gccgctcgag acgcggatca ggtccgggtt gtggctgagg ttgaggtcga tgaactggcg
     4681 caacagcgcc tcgttgaggt ggccgtcctc ggtcaggatg aacgaatcgt agaggctcgc
     4741 caccagcagg atgtgctgca cccggtggcg catcaggtcc tggaagcgcc gcaggcgacg
     4801 ctggggccgc tcggcccctc gtgggttgtg cgggacgctc acgaccgctc tccggcaatc
     4861 acgggccagg ccgacacacg gacggcgccc gggatgggcg ccgtccgatt ctaacccgtg
     4921 gcggctccgc tcgcgttcag accacgccct ggtccagcat cgagtcggcg accttgagga
     4981 aaccggcgat gttcgcgccg ttgacgtagt tgcccggcgt gccgaatcgg tcggcggccg
     5041 ccacgcacga ctcgtggatg ctcttcatga tcagttgcag gcggttgtcc acctcgtcgc
     5101 gggtccagct gtagcgcatg ctgttttgcg ccatctcgag accggagacc gccacaccgc
     5161 cggcgttggc ggccttcccc ggaccgtaca gcacgcgcgc ctcgacgaac agcgacacgc
     5221 cgtcggcggt cgtcggcatg ttggcgccct cggagatcag gtagcacccg ttgttcaaca
     5281 ggttctgcgc gtccttgccg ttgatctcgt tctgcgtcgc gctggggaat gcgcagtccg
     5341 ccttgtggcg ccacagcgga ttgtaatcgc cgccggcatc cgccgcggtg taggtcgccg
     5401 acgagaactt gtcggcgtat tccttgatcc gcccccgtcg gacgttcttc aggttgatca
     5461 cgaactcgag cttgtccgtc gtgatgcctg cctcgtcgta gatgtagccg cccgagtcgg
     5521 acagcgtcag caccttcgcc cccagctgca gcagcttctc ggtcgtgtac tgcgcgacgt
     5581 tgccgctgcc ggagacgaga cagcgtttgc cctcgagcgt ctgcttgcgc gtcgccagca
     5641 tctcggcggc gaagtagacc gcgccgtacc cggtggcctc gggccggatc agcgaaccgc
     5701 cccagttcag ccccttgccc gtcagcacgc cgctgaactc gttggccagc cgcttgtatt
     5761 gcccgaacag gaagccgatc tcgcggccgc cgacgccgat gtcgccggca ggcacgtcgg
     5821 tgttctggcc gatgtgacgg aacagctcgg tcatgaagct ctgacagaag cgcatcacct
     5881 cctggtcgct cttgcccttg gggtcgaagt ccgacccgcc cttgccgccg cccatcggca
     5941 aggtcgtcag cgagttcttg aacacttgtt cgaaggcgag aaacttgagg atgccgaggt
     6001 tgacagacgg atggaagcgc aggccccctt tgtaaggacc gatggcgctg ttcatctcga
     6061 tccggtagcc gcggttgacc tgcacgttgc cctggtc
//
LOCUS       DASPGN010000234         4941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1094484, whole genome shotgun sequence.
ACCESSION   DASPGN010000234 DASPGN010000000
VERSION     DASPGN010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094484"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VD788_06780"
     CDS             complement(<1..323)
                     /locus_tag="VD788_06780"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76012.1"
                     /translation="MPDALGWTVFGILVAVVLGIDLAVAHRKPHAVTLKEATVWSSVW
                     VALALLFGAGVWITRGSRAGLEFYTGWLIEEALSVDNLFVFVVLFGYFHVPARFQHRV
                     LFWGVL"
     gene            582..905
                     /locus_tag="VD788_06785"
     CDS             582..905
                     /locus_tag="VD788_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76013.1"
                     /translation="MSPFIAPTVSAALSVLVCAAPLGAEDSIARVHEFVEIYNTHAAP
                     ALRSFIRDHYDERSLDSSPRAEEVFADRWLRAFSLVGPVSVRHLERDGACSASRPAAS
                     SREPG"
     gene            860..2104
                     /locus_tag="VD788_06790"
     CDS             860..2104
                     /locus_tag="VD788_06790"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXV76014.1"
                     /translation="MLRVSTRGILTRTWLTFTFGLDQVGRIDQQEIVSTWRSARIRPS
                     GPPITPDELPAYLDPYLVGLAAADMFSGVVVLIDDGKVRWEFEAGFTGAEMDVPIDAD
                     TRFDLASVSKMFVATAALQLREKALLDFSSPIVEYLPDYPAHIGERINVEQLLSHTTG
                     LRLQEDEQLVQRLFRAQSFAEQFAIETQALAAAYDAEFRPLGTYLYSNENMDLAARII
                     EVAAGQGYYDYLQTRVFAPLEMTRTHPYNAGRDAGTVALGLTRQHPPDGTFVNGPRRG
                     NRHFLFDAGRPAGGLVTSARDLARYAIGLTSGSLMSSEAWAEMTRPRALQQETTDFRL
                     DHGLGIDVYHYPSGVVLGHGGGRPGASTRLDIFPQQGVALVVLSNYDYIAHRIAEHVQ
                     EVLMVGRFDEPPAGAPPGEDAP"
     gene            2299..2973
                     /locus_tag="VD788_06795"
     CDS             2299..2973
                     /locus_tag="VD788_06795"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HXV76015.1"
                     /translation="MGDATPRTDLRSEATRSALIRSAIDAYGRAGFHGTTTRAIARAA
                     AVNQAAIAYHFGGKAGLYHASIEHIAERIGGFLGAVTIEAHRQLDHLAASEPDEPTLR
                     HGCLEVAGRLTDALARMLTGEESESWARLIVREQQDPTESFDLLQGVVGPWAAALIRV
                     FESAGGQTPAEARLSVLTIVGQALVFRVSRTSVLRITGWKQIGERELAAIQRRIRQNV
                     RAVLVR"
     gene            2970..4475
                     /locus_tag="VD788_06800"
     CDS             2970..4475
                     /locus_tag="VD788_06800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV76016.1"
                     /translation="MRADRERRETMCGRSGTTAARALAVLVGPALLAGCLKVGPDYAP
                     EDLTDRYRDGFRTGATAGETTSLATSAPVGEWWRQFEDPELERLVQALVGGNLDLAAA
                     RERIVEARARRGIVRADRLPHVDLEGQAQAAGTGEEALNFVGPPPGEEAEIYAAGALA
                     GWEIDLWGRVGRLVEAADREIEARVADYGDVVVSLAAELALAYVDARTLEQRIDMLDR
                     NVELLESSLEIVESRHRAGIGTELDVRQSRRVLERTRAERPPLLRAREVAYNRIAVLI
                     GEPPVDGVITAGELPRPPGLVGIGIPADLLARRADVRRADRDYAAAVARVGSTMAERY
                     PRLSLFGSLKFQTDDAGALLDPEAMVFSIGPTLRWPLFAGGQISSNVRVRESQAEQAR
                     LLLERTVLEAVREVDDAAVGVAQNQRRVLRLRQAAADARHSVALSEQLYKAGLGSLLQ
                     LIDAQRALVAVEDELLVARQDELGGIVRLYRTLGGGWESLPPAVATGGAER"
     gene            4472..>4941
                     /locus_tag="VD788_06805"
     CDS             4472..>4941
                     /locus_tag="VD788_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HXV76017.1"
                     /translation="MKRSRVAVPVVLVLLAAGIAGLVWWYGRPAGTDSDSLTLYGNVE
                     LRDAELAFIEQERVAEVLVEEGQAVERGQLLARLARELLDAQLAEAAAQLTAEREALR
                     RLTNGTRPQEIEQAEARLQAAVVRASNAERLVRRLEETTATGASTEQELDDARSR"
BASE COUNT          771 a         1695 c         1744 g          731 t
ORIGIN      
        1 agcacgcccc agaagagcac ccgatgctgg aaccgggcgg gcacgtggaa gtacccgaac
       61 agcacgacga acacgaagag gttgtcgacc gacagcgcct cttcgatcag ccagcccgtg
      121 tagaactcga ggccggcccg cgagccccgc gtgatccaga cgccggcgcc gaagagcagg
      181 gcgagcgcca cccagacgga cgaccagacg gtggcctcct tgagggtgac tgcgtgcggt
      241 ttgcgatggg cgacggccag gtcgatcccc agcacgacgg ccaccaggat cccgaagacc
      301 gtccagccga gagcatcggg catgcaagag cctccgaacc gccggaaccc gtccggccgt
      361 tgcgaaggtc ttgcccggtc ctgacctgac ctgccgcacc gcgcgccttc ggcgcggtcc
      421 tgacgatgcg gcatcgcggg cggcagggac gccgcccgcg gggctactcc ccttcggggc
      481 gaggaggaat ctaccacagc gccggtgccg gcgaccatcg tcgtcgcatc ggccacggca
      541 cattggtgta ttttcatccg tatgcccgag gcatcgaaac cgtgagcccc ttcatcgcgc
      601 ccacggtgtc cgccgccctc tcggtcctcg tctgcgccgc gccgctgggc gccgaggact
      661 cgatcgcacg cgtccacgag ttcgtcgaga tctacaacac acacgccgct cccgcgctgc
      721 ggagcttcat ccgcgaccat tacgacgagc gctccctcga cagctcccca cgagccgaag
      781 aggtgttcgc cgatcgctgg ctgcgggcgt tttcgctggt cggtcccgtt tccgtgcgcc
      841 atctcgaacg cgacggggcg tgctccgcgt ctcgacccgc ggcatcctca cgcgaacctg
      901 gctgaccttc accttcggcc tggatcaggt cggccgcatc gatcagcaag agatcgtctc
      961 gacttggcga tccgccagga ttcgaccgtc cggaccgccg atcacaccgg acgagcttcc
     1021 tgcctacctc gacccgtacc tggtcggact cgcggccgcg gacatgttct cgggagtcgt
     1081 ggtgctcatc gacgacggga aagtgcggtg ggagttcgag gccgggttca ccggcgccga
     1141 aatggacgtg ccgatcgacg ccgacacccg gttcgacctc gcctcggtca gcaagatgtt
     1201 cgtcgccacc gcagccctac agttgaggga gaaagctctc ctcgacttct cgagcccgat
     1261 tgtcgagtac cttcccgatt atcccgcgca catcggcgag cggatcaacg tcgaacagtt
     1321 gctctcgcac accaccggat tgcgcctaca agaagacgag cagttggttc agcgcctgtt
     1381 ccgcgcgcag agtttcgccg agcaattcgc gatagagacc caagccctgg ccgcggccta
     1441 cgacgcggag ttccgcccgc tcgggaccta tctgtactcc aacgagaaca tggatctggc
     1501 cgcgcgcatc atcgaggtcg ccgccggcca gggctattac gactatctgc agactcgagt
     1561 gttcgctccg ttggagatga cacggaccca tccgtacaac gccggccgtg acgccggcac
     1621 cgtagctttg ggcttgactc gccaacatcc tcccgacggc acgttcgtca atggcccccg
     1681 acggggcaat cgccattttc tgttcgacgc cggccggccg gcgggtggac tcgtgacctc
     1741 ggcccgggac ctggctcgct atgccatcgg actgacgagc gggagcctaa tgtcgagcga
     1801 ggcgtgggcg gagatgactc gtccccgtgc gctgcagcag gagacgaccg acttcaggtt
     1861 ggaccatggc ctcggcatcg atgtctacca ctacccgagc ggcgtcgtgc tgggccacgg
     1921 cggaggccgg ccgggagcca gcacgcgtct cgacatcttt ccgcagcagg gggtcgcgct
     1981 cgtcgtgttg agcaactacg actacatcgc ccacaggatc gccgagcacg tccaggaggt
     2041 cctgatggtg ggccgattcg acgagccgcc cgccggtgcc ccgccgggag aagatgcccc
     2101 ttgacatacc gtttgcaacg acgtgccgca gatcgccccg cgcaaggcgc gggcggtcaa
     2161 gagccgggcc gtacgggcgc tcgtccgagc cgcgctcgcc gagcggcgtc cgccgcgaag
     2221 cgccgatcga acggcacgtc gggaaaaaaa tagaatcaat cgattgacta attcattcga
     2281 ttggttagta taggtggcgt gggcgatgcc actccacgca ccgatctccg cagcgaagcc
     2341 actcggagtg ccctcatccg gagcgcgatc gatgcctacg gccgcgcggg cttccacgga
     2401 acgacgacac gcgcgatcgc gcgtgccgcc gcggtcaacc aggcggcgat cgcctaccac
     2461 ttcggcggga aggcgggtct gtaccacgcc agcatcgagc acatcgcgga gcggatcggg
     2521 ggcttcctcg gggcggtgac gatcgaggcg caccggcagc tcgaccacct cgccgcgagc
     2581 gagcccgacg agccgacgct gcgccacggc tgtctcgaag tcgccggacg gctgaccgat
     2641 gcgctggccc gcatgctgac cggcgaggag tcggaatcgt gggcgcgcct gatcgtgcgc
     2701 gagcagcagg atccgaccga gtcgttcgac ctgctgcagg gcgtcgtcgg tccgtgggcc
     2761 gcggcgctga tccgcgtctt cgagagcgcc ggcggccaga cgcccgccga ggccaggttg
     2821 tccgtgctga cgatcgtcgg gcaggcgctc gtttttcgcg tgtctcgcac gagcgtcctg
     2881 cgcatcacgg gctggaagca gatcggcgaa cgcgagctgg ccgcgatcca gcggcgtatc
     2941 cggcagaacg tccgggcggt gctggtgcga tgagagccga ccgcgagcgg agggagacga
     3001 tgtgcgggag atccggcacg acggcggcgc gcgcgctcgc cgtcctcgtc gggcctgcgc
     3061 tcctcgccgg ttgtctcaag gtcggtccgg actacgctcc ggaggacctg acggaccgtt
     3121 accgtgacgg gtttcggacc ggggcgactg caggggagac gacctccctc gcgacatcgg
     3181 cgccggtagg cgaatggtgg cggcagttcg aggatcccga gctcgagcgg ctcgtgcagg
     3241 ctctcgtcgg cggcaatctc gacttggcgg cggcgcgcga gcgcatcgtc gaggcccggg
     3301 cgcggcgcgg catcgtccga gccgaccgcc tgccgcacgt cgatctcgag gggcaggcgc
     3361 aggccgcggg cacgggcgag gaggcgttga acttcgtcgg cccgcccccc ggggaggagg
     3421 cagagattta tgccgcggga gccctcgccg gctgggagat cgacctgtgg gggcgcgtgg
     3481 gccggctggt cgaggccgcg gaccgtgaga tcgaggcccg cgtggccgac tacggcgacg
     3541 tggtcgtgtc gctcgcggcc gagctggcgc tggcctacgt cgacgcgcgt acgctcgagc
     3601 agcggatcga catgctcgac cgcaacgtcg agctgctcga gagctcgctc gagatcgtcg
     3661 agtcgcgcca tcgcgccggc atcgggaccg agctcgacgt ccggcagtcg cgccgggtgc
     3721 tcgagcgaac ccgcgccgag cggccgcccc tgctgcgggc gcgcgaggta gcctacaacc
     3781 gcatcgcggt gttgatcggc gagccgccgg tcgacggcgt gatcacggcc ggcgagctgc
     3841 cgcgcccgcc gggcctggtc gggatcggga ttcccgcgga cctgctcgcg cggcgcgcgg
     3901 acgtgcggcg cgccgatcga gactacgccg cggccgtcgc gcgcgtcgga tcgacgatgg
     3961 ccgagcgcta tccgcggctc agcctgttcg gctcgctgaa gttccagacc gacgatgccg
     4021 gcgcgctgct cgatcccgag gcgatggtct tctcgatcgg tccgacgctg cgctggccgc
     4081 tgttcgccgg cgggcagatc tcgagcaacg tccgcgtgcg cgagtcgcag gcggagcagg
     4141 cccggctgct cctcgagcgc acggtgctcg aggcggtgcg cgaggtcgac gacgccgccg
     4201 tgggggtcgc gcagaaccag cgccgggtgc tccggctgcg ccaggccgcc gccgatgcgc
     4261 gtcacagcgt ggccctttcc gagcagctct acaaggcggg tctgggcagc ctgctccaac
     4321 tcatcgacgc ccagcgcgcg ctcgtggccg tggaagacga gctgttggtt gcccggcagg
     4381 atgagctggg tgggatcgtg cgtctgtatc gcacgctcgg cgggggatgg gagtcccttc
     4441 cgccggcagt cgcaaccgga ggcgccgagc gatgaagaga agccgtgtcg cggtcccggt
     4501 cgtactggtc ctgctcgccg ccggcatcgc ggggctcgtc tggtggtacg gccgaccggc
     4561 cgggacagac tccgactcgc tgacgctgta cggcaacgtc gagttgcgcg acgccgagct
     4621 ggctttcatc gagcaggagc gggtggccga ggtgctggtc gaggagggcc aggcggtcga
     4681 gcgcggacag ctgttggcgc ggctcgcgcg cgagctgctc gacgcgcagc tcgccgaggc
     4741 cgcagcacag ctcacagccg agcgggaagc gctgcgccga ctgaccaacg gcacgcgccc
     4801 gcaggagatc gagcaggccg aggcgcggct ccaagcggcg gtcgttcgcg cgagcaacgc
     4861 ggagcggctg gtccgacggc tcgaggagac gacggccacc ggcgcgagca ccgagcagga
     4921 gctcgacgac gcccgctcgc g
//
LOCUS       DASPGN010000235         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_742862, whole genome shotgun sequence.
ACCESSION   DASPGN010000235 DASPGN010000000
VERSION     DASPGN010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742862"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VD788_06810"
     CDS             <1..618
                     /locus_tag="VD788_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76018.1"
                     /translation="RPATLRTNPSRTDGADPRVSIVVGLTGSNAAGKGAVAEYLRESG
                     FAVHSLSDVIREVAAERGLPPLREHLIRIGNELRRGGGAGALAVAIRPRLGRRDVVDS
                     IRNPAEVEVLRTIPGFVLVGVRAPIELRFERSRVRGRPGDPESLAQFASREAQENTDD
                     PDAQQLRATFLLADEVLDNQGDLSALHHAIDELLSRRVGSAPRRS"
     gene            693..2195
                     /locus_tag="VD788_06815"
     CDS             693..2195
                     /locus_tag="VD788_06815"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HXV76019.1"
                     /translation="MRVRVSSKDPSEIEADVVVCFAYEDATAPGAVENPRLLRALTAE
                     MKAERFRGQPTERVMWNTDGDFPCQRFIVIGLGPRSDVPGETIRAGAARAVRAASRCS
                     ARRLALRLPDPDARDPGQEVRAAVEGALLGNYHFDRHLTDPSRKVFRPRSLEVSVARD
                     TRGLAREAHRGEITAAATCLARDLVNEGPSRMNPEGLARAALHAARAAGIGCEVIQAA
                     ELKRLGMTALLAVARGSRQAARVVHLTYRPTGRRAAERKPIVLVGKGVTFDSGGLNLK
                     PSDGMLTMKSDMAGAAAVLAVMSALPEIGCKSEVHALLGLVENMTGADAYKPGDILDS
                     YSGKTVEVMNTDAEGRLVLCDLLAYAASRLDPRCVVDVATLTGACVVALGTRASGLLS
                     RHDELAERILAAARTAGERVWRLPLYDEYLQLLQRGPADLKSTGSRWGGAITAGLFLG
                     EFVPRELPWAHLDIAGPAFTETELPESRVGGTGVAVSTLIRWLEADRSAS"
     gene            2192..2707
                     /locus_tag="VD788_06820"
     CDS             2192..2707
                     /locus_tag="VD788_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HXV76020.1"
                     /translation="MSHRPEAPSIRASVALLTVSDTRTVDTDRSGQLAKRLLEEAGHR
                     SFDYRIVPDEPERVREQIERWLGQDACDAVVVNGGTGIAARDRTYEAVVALLDQRLPG
                     FGELFRALSFVEIGSGAMLSRAVAGVARGRPLFSLPGSPAAVELALRRLIVPELGHLL
                     GELRKQPPRPR"
     gene            complement(2722..>2924)
                     /locus_tag="VD788_06825"
     CDS             complement(2722..>2924)
                     /locus_tag="VD788_06825"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HXV76021.1"
                     /translation="SHTREEFLMDFINLFPPEGVVNARVIVSPGHLKRMIRALSDNLA
                     RYEAKFGPVIEARAPDPDTLTN"
BASE COUNT          408 a         1015 c         1059 g          442 t
ORIGIN      
        1 cgtccggcga cgcttcggac gaatccgagt aggaccgacg gggccgaccc gcgcgtgtcg
       61 atcgtcgtcg ggttgaccgg ctcgaacgcc gcgggcaagg gtgcggtcgc cgaatacctg
      121 agagaatccg gctttgccgt ccactcgttg tcggacgtga tccgcgaggt cgcggccgag
      181 cgcgggctcc ctccgctgcg cgagcacctg atccgtatcg gcaacgagct tcgtcgcgga
      241 ggcggcgccg gggcgctggc ggtcgcgatc cgtcctcggc tggggcgacg cgacgtcgtc
      301 gattcgatcc gcaacccggc cgaggtcgaa gtgctgcgga cgatccccgg cttcgtgctg
      361 gtcggcgtgc gggcgccgat cgagctccgc ttcgaacggt cgcgggtccg tggacgccct
      421 ggcgacccgg agtccctcgc gcagttcgcc tcgcgcgagg cgcaggagaa caccgacgac
      481 ccggacgccc agcagctgcg ggctaccttc ctgctcgccg acgaagtgct cgacaaccag
      541 ggcgacctga gcgccctgca tcacgcgatc gacgagctgc tgtcgcgacg ggtcggatcg
      601 gccccccggc gtagttgacc gctccgccgc gcctccggta gattctccga ccgaccacac
      661 cccgatccgc gcatcgacgc gccggagatt ggatgcgagt ccgagtcagt tcgaaggacc
      721 cgtcggagat cgaggcggac gtcgtcgtct gcttcgcgta cgaggacgcg accgcgccgg
      781 gagccgtcga gaatccccgg ctgctccgcg cgttgaccgc cgagatgaag gccgagcggt
      841 ttcgcggtca accgacggag cgggtgatgt ggaacaccga cggcgacttc ccgtgccaac
      901 ggttcatcgt catcggcctc ggtccgcgga gcgacgtgcc gggcgagacg atccgcgccg
      961 gcgcggcccg tgcggtccga gccgcgtcgc ggtgctcggc gagacggctc gcgttgaggc
     1021 tgcccgaccc ggacgcgcgc gatcctggcc aggaggtccg cgcggcggtc gaaggcgcgc
     1081 tgctcggcaa ctaccacttc gaccgccatc tcaccgaccc gtcgcgcaag gtgttccgac
     1141 cgcgttcgct cgaggtttcg gtcgcgcgcg acacgcgtgg cctggcgcgc gaggcgcacc
     1201 gtggcgagat caccgccgcg gccacctgct tggcccgcga cctggtcaac gaggggccgt
     1261 cgcggatgaa cccggagggg ctggcccgcg ccgcgctgca cgcggcccgc gcggccggca
     1321 tcggctgtga ggtgatccag gcggcggagc tgaagcggtt gggcatgacg gcgctgttgg
     1381 ccgtcgcccg gggctcgcgt caggcggcgc gggtcgtgca cttgacctac cgtccaaccg
     1441 gccgtcgcgc cgccgagcga aagccgatcg tgctcgtcgg caagggtgtc accttcgaca
     1501 gcggcggtct gaacctgaag ccgagcgacg gcatgttgac gatgaaatcc gacatggcgg
     1561 gtgctgcggc ggtgctcgcc gtgatgagcg ccctcccgga gatcggctgc aagagcgagg
     1621 tgcacgcgct gctcggcctc gtcgagaaca tgaccggcgc cgacgcctac aagcccgggg
     1681 acatcctcga cagctattcc ggcaagacgg tcgaggtgat gaacaccgac gccgagggcc
     1741 gcttggtcct gtgcgacctg ctggcctacg ccgcctcgcg gctcgacccg cgatgcgttg
     1801 tcgacgtcgc gacgttgacc ggggcctgcg tcgtcgcgct cgggacgcgg gccagcggct
     1861 tgctctcccg acacgacgag ctcgccgagc ggatcctggc ggcggcccgg accgccggcg
     1921 agcgcgtgtg gcggctgccg ctctacgacg agtacctgca gctgctgcag cgcgggccgg
     1981 ccgacctgaa gagcaccggc agtcgttggg gtggggcgat caccgccggg ctgttcctcg
     2041 gcgagttcgt gccgcgagag ctgccgtggg cccacctcga catcgcgggt cccgcgttca
     2101 ccgagaccga gctgccggaa tcccgcgtcg gcggcaccgg tgtcgcggtc tcgacgctga
     2161 tccgctggct cgaagcggac cgttcggcgt cgtgagtcat cggcccgagg ctccgtcgat
     2221 ccgcgcgagc gtcgcgctgc tgacggtcag cgacacgcgg acggtcgata ccgatcggtc
     2281 ggggcagctc gccaagcgcc tgctcgagga ggccggccac cggtcgttcg actaccgcat
     2341 tgtcccggac gaaccggagc gggtccgcga gcagatcgag cgctggctcg ggcaggatgc
     2401 atgcgatgcg gtcgtggtca acggcggcac ggggatcgcg gcgcgagacc gcacctatga
     2461 agccgtcgtc gcgttgctcg accagcgctt acccggcttc ggcgagctgt tccgcgcact
     2521 gtcgttcgtc gagatcggca gcggcgcgat gctgagccgc gccgtcgccg gcgtcgcacg
     2581 cggccgcccg ctgttttcgc tgccgggctc gccggccgcg gtcgagctgg cgttgcgccg
     2641 gttgatcgtc cccgaactgg gtcacctgct cggcgagctg cgcaaacagc cgccgcggcc
     2701 gcgctgagtc accaaggccg gtcaattggt cagcgtatcc ggatcgggcg cacgggcctc
     2761 gatcaccgga ccgaacttgg cctcgtagcg cgcgaggttg tcgctcagtg cgcggatcat
     2821 cctcttcaag tggcccggac tgacgatcac ccgcgcgttg accacgcctt ccggcgggaa
     2881 caggttgatg aagtccatca ggaactcttc ccgagtgtga gaga
//
LOCUS       DASPGN010000236         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1562595, whole genome shotgun sequence.
ACCESSION   DASPGN010000236 DASPGN010000000
VERSION     DASPGN010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562595"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VD788_06830"
     CDS             <1..537
                     /locus_tag="VD788_06830"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV76022.1"
                     /translation="EQEVDNADGIIGDLLEMTRAKAPVKAPVDLGEVVTAAFSRLQSE
                     RLSLDLAIDPQPFRVDADAGQLRQVFTNLLLNAAQAMDGRGTVRVRARHFGGQALVTV
                     EDEGAGIRPEDRERVFEPLFTTKAKGTGLGLPVCRQIIEGHGGTIAVADTSSAGTRFE
                     IRLPEVPEDHWGGTEQRR"
     gene            534..926
                     /locus_tag="VD788_06835"
     CDS             534..926
                     /locus_tag="VD788_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV76023.1"
                     /translation="MSRTRVLVVDDEINMRETLRDILVEEGFEVSIAETGEKAVKICK
                     SSRFDAVLMDVRMPGIDGIEASRRIHRIAGETRVIIMSAYSVEHLIDASSADGIVGFL
                     RKPLDAVELLRLLGDTRNGDSPELGEQR"
     gene            923..2128
                     /locus_tag="VD788_06840"
     CDS             923..2128
                     /locus_tag="VD788_06840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV76024.1"
                     /translation="MTDLLEQRPLANILVVEDDDAQRQTLCDILTQEGFVPIACATAT
                     EALESAAASNASVAIVDLRLPDMTGVQVLERLMQLLHGARVIIHTAYGSFESARDALN
                     LGAFAYVEKLSDPRELVSHVHRAMDELLAGVLRDGDRELRSLTEHVPDVVFQVDVGGR
                     ILFVNRPMTGRSTEQLLGEHLHDWVLPDHQETLDAAVSAVFDNYRPQECEVVSEAADG
                     SRYWYSCRIGPIGEPTHVERAIIIARDITEARQAEQIERVVLQLFRNAREAIEGGGKL
                     MVEVEPVVLEDDHSRTQPEVRSGPYVLITVTDTGIGIAESQIDRIFEPFFTTKPGHSK
                     GLGLTEVYGIVAQAGGHMSVESEPGEGTVFRVYLPSAYPAGLARGGESGLRASPNVVY
                     RFPNPKGQR"
     gene            2125..2541
                     /locus_tag="VD788_06845"
     CDS             2125..2541
                     /locus_tag="VD788_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76025.1"
                     /translation="MTPDAELYELPEFSAILSFIDAIFSELNMGLLIYHVEEPRDIRT
                     ARLIYANKTASAYTGADLKHQVGKTIFDAFPQLVDTDAPRIYFEVVNDKQARNAGIIE
                     YQDRRVHHGRYATRAFPMPNDCIGVLFDKLGDRPAS"
     gene            complement(2655..3329)
                     /locus_tag="VD788_06850"
     CDS             complement(2655..3329)
                     /locus_tag="VD788_06850"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXV76026.1"
                     /translation="MHAAPYPLGSRKSDKPLLIGAAIVAVVIHLVILFGVVLPDLRAP
                     EPPVVSEPTITLQRTVLPAPPIDRLAPRRASESHRRAPVPDRIAPPVEPLREPPADIT
                     AFAAPEIPDPDVGEPTPPFDAGPIDPRTPGLTPPRLIATTRVDPDYPEAGRVARRDAH
                     VELHAEILASGEIGAIEIVRCDAPGLGFEQAAARAVARWRYEPAVLHGSRVATFVTVY
                     VDFTLH"
     gene            complement(3438..>3777)
                     /locus_tag="VD788_06855"
     CDS             complement(3438..>3777)
                     /locus_tag="VD788_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXV76027.1"
                     /translation="GPGLERDYAAASGRRLDVPRIAEQAGDGDAQAVAALARYESRMA
                     RALATVINLLDPDVIVLGGGLSNLERLYDAVPRRWQRWVFSDRVDTRLVRPVHGDASG
                     VRGAAWLWHG"
BASE COUNT          633 a         1256 c         1308 g          580 t
ORIGIN      
        1 gagcaggagg tggacaacgc cgatggtatc atcggcgact tgctggagat gactcgggcc
       61 aaggccccgg tcaaggcgcc ggtcgacttg ggtgaggttg tcaccgccgc tttctcacgg
      121 ctgcagtcgg agcggttgtc gctcgacctc gccatcgacc cgcaaccgtt ccgcgtcgac
      181 gccgacgcgg ggcagttgag gcaggtgttc accaacctcc tgctcaacgc ggcgcaggcg
      241 atggacggcc gcggaacggt ccgcgtgcgc gcccgtcatt tcggtggcca agcgctcgtc
      301 accgtcgagg acgaaggcgc cggcatccgt cccgaggatc gcgagcgcgt gttcgagccg
      361 ctgttcacga ccaaggccaa gggaaccggc ctggggttgc cggtgtgcag gcagatcatc
      421 gagggccacg gcggtacgat cgccgtcgcc gacacgtcgt ccgccggcac gcgtttcgag
      481 atccggctgc cggaggtgcc ggaagatcat tggggcggca cggagcaacg gcgatgagtc
      541 gcactcgcgt tctcgtcgtc gacgacgaga tcaacatgcg tgagacgcta cgcgatatcc
      601 tggtcgagga aggcttcgag gtctcgatcg ccgagaccgg agaaaaggcg gtcaagatct
      661 gcaagagctc gcgcttcgac gcggtgttga tggacgtccg gatgcccggc atcgacggga
      721 tcgaagcgtc gcggcgcatc caccggattg ccggtgagac gcgggtaatc atcatgagcg
      781 cgtacagtgt cgagcacctg atcgacgcct cctccgccga cgggatcgtc ggtttcctgc
      841 gcaaaccact cgatgccgtc gagctgctga ggctgctcgg cgacacacga aacggcgatt
      901 cgcccgagtt gggagaacag cgatgaccga cctactcgaa cagcggccgc tcgccaacat
      961 tctggtcgtc gaggacgatg acgctcagcg tcagaccctg tgtgacatcc tgacccaaga
     1021 ggggttcgtg ccgatcgcgt gcgcgaccgc gaccgaggca ctggagtcgg cggcggcgtc
     1081 gaacgcgtcg gtcgcgatcg tcgacctgcg cctgcccgac atgacggggg ttcaggtgct
     1141 ggagcggctg atgcagctgc tccacggcgc gcgcgtgatc atccacaccg cctacgggtc
     1201 gttcgaatcc gcccgagacg cgctgaatct cggagcgttc gcctacgtcg agaagctgag
     1261 cgacccgcgc gagctggtca gccacgtcca ccgagcgatg gacgagctgc tcgccggcgt
     1321 gctgcgcgac ggcgatcgtg agctgcggtc gctcaccgag cacgtcccgg acgtggtgtt
     1381 ccaggtcgac gtcggcggtc gcatcctgtt cgtcaatcgt ccgatgaccg gccgctcgac
     1441 cgagcaactg ctcggcgagc atctccacga ctgggtgctg cctgaccatc aggagacgct
     1501 cgacgcggcc gtcagcgcgg tgttcgacaa ctatcgcccg caagaatgcg aggtggtgag
     1561 cgaggcggcc gacggctcgc gctactggta ctcctgccgc atcggaccga tcggagaacc
     1621 gacacacgtc gaacgggcga tcatcatcgc gcgcgacatc accgaggccc gccaggccga
     1681 gcagatcgag cgcgtcgtcc tgcagttgtt ccgcaacgcg cgcgaggcaa tcgagggcgg
     1741 gggaaagctg atggtcgagg tcgagccggt cgtgctcgaa gacgaccact cgcggacgca
     1801 acccgaggtg cgcagcggcc cgtacgtgtt gatcacggtc accgacaccg gcatcggcat
     1861 tgcggaatcg cagatcgacc gcatcttcga accgttcttc acgacgaagc cggggcactc
     1921 caaggggctc ggtctgaccg aggtatacgg gatcgtcgcc caggcggggg ggcacatgtc
     1981 ggtcgagagc gagcccggcg agggaaccgt cttccgtgtc tacctgccga gcgcctaccc
     2041 ggcgggtctg gcccgcggcg gggaatccgg cctgcgcgcg tcgcccaacg tggtataccg
     2101 ctttccgaac ccgaaggggc aacgatgaca ccggatgccg agctctacga gctgccggag
     2161 ttttccgcga tcctcagctt catcgacgcg attttctccg agctcaacat ggggctcttg
     2221 atctatcacg tcgaggagcc gcgggacatc cgcacggcga gactgatcta cgccaacaag
     2281 accgcatcgg cctataccgg cgccgatctc aagcaccagg tcggcaagac gatcttcgat
     2341 gcgttccccc agctcgtcga caccgacgcg cctcggatct acttcgaagt cgtcaacgac
     2401 aagcaggcgc gcaacgccgg aatcatcgaa taccaggatc ggcgagtcca ccacgggcgc
     2461 tacgcgacgc gcgcctttcc gatgcccaac gactgcatcg gtgttctgtt cgacaagctc
     2521 ggggacagac ccgcgagctg acgccatcgt ggttcgtggg acggcgcgga ctccaattcg
     2581 gtggagggcc ggtagccgcc ggggctcgtt accgcgaccc gccccacacc gcgattatac
     2641 gatcggcggg cgcttcaatg tagcgtgaag tcgacataca cggtcacgaa tgtggcgacc
     2701 cgcgagccgt ggagcacggc gggttcgtag cgccaccggg cgaccgcccg cgcggcggcc
     2761 tgttcgaacc cgaggccggg agcgtcgcag cgcacgatct cgatcgcccc gatctccccg
     2821 ctggcgagga tctcggcgtg cagttcgacg tgggcgtcgc gccgcgcgac ccttcccgct
     2881 tccgggtagt ccgggtcgac ccgcgtcgtc gcgatcaacc gcggaggggt gagccccggg
     2941 gttcgcgggt cgatcggtcc ggcgtcgaac ggcggcgtcg gctcgccgac gtccgggtcc
     3001 ggtatctccg gcgccgcgaa cgccgtgatg tcggcaggtg gctcgcgcag cggttcgacc
     3061 ggcggtgcga tccggtccgg caccggtgcc cggcgatgcg attcgctcgc gcgacgaggc
     3121 gccagccggt cgatcggcgg cgcggggagc accgtccgct gcagtgtgat cgtcggctcg
     3181 gataccacgg gtggctcggg cgcgcgcagg tcgggcagca cgacgccgaa caggatgacg
     3241 aggtggatca cgacggcgac gatcgccgca ccgatcagca ggggtttgtc cgatttcctc
     3301 gacccgagag ggtagggcgc agcgtgcatc ggcgacctcg tcccccccgg gagtattccg
     3361 ccacgacgac aagcttaggt tccggtgcgg ctcggggcga gcgggtcgcg gaaatcccgc
     3421 ggcatcgata cagcgattca gccatgccac agccacgcgg cgccccggac gccgctggca
     3481 tcgccgtgca ccgggcggac caggcgggta tcgacgcggt cgctgaagac ccatcgctgc
     3541 cagcggcgcg gcacggcgtc gtacagccgt tcgaggttgg acaggccccc gccgagcacg
     3601 atgacgtcgg gatcgagcag gttgatcacc gtcgcgagcg cgcgcgccat tctcgattcg
     3661 tagcgcgcga gcgccgcgac ggcctgcgcg tcgccgtcgc cggcctgctc ggcgatccgg
     3721 ggtacgtcga gccgacggcc ggaggccgcc gcgtaatcgc gctcgagccc gggcccc
//
LOCUS       DASPGN010000237         6447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1328347, whole genome shotgun sequence.
ACCESSION   DASPGN010000237 DASPGN010000000
VERSION     DASPGN010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328347"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..495
                     /locus_tag="VD788_06860"
     CDS             79..495
                     /locus_tag="VD788_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HXV76028.1"
                     /translation="MRCPRCQTVLERRDHDGVRVDTCATCAGVFLHRGELNRIAPTEG
                     DLEFSTLHEESFRHEDEFGPIACPACSGPTMNKVEFNVYSGIMLDYCPACRGFWLDGK
                     ELDRIVEEVRGLDRPEDDVGAGPLQWFAQFLWSLPR"
     gene            581..1495
                     /locus_tag="VD788_06865"
     CDS             581..1495
                     /locus_tag="VD788_06865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXV76029.1"
                     /translation="MVTANDPRDLAARIVESSAAAIVVTDAGARIRFVNPAFTRITGY
                     PPDEVLGRSPRLLQSGRHSPKFYQRMWRRLTHRRRWHGEIWDRRKNGEVFPMRMSVTA
                     IDERGATLYVGVFEEIAAEKRREERLCRLAFYDPLTGLPNRTLLADRLERGLVQAKRS
                     GRCLAVLFVDVDGFKHVNDRFGHAIGDRVLARVGRTLTLGLRRSDTVARVGGDEFVVL
                     LPDLDSRRQAIPVARKILRSLSAGLTVGGRELAVGASIGISSYPDDARDARQLLRLAD
                     SAMYRLKRERRGARPRPRPSRGVLQSDG"
     gene            1492..2958
                     /locus_tag="VD788_06870"
     CDS             1492..2958
                     /locus_tag="VD788_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HXV76030.1"
                     /translation="MIRRASASVAVALWIVDAALGPAAGQSPAAPDPASSFAERLERN
                     PLDRQAMVGRVEALLASDRWRTALEEARAYSAVAPDDRHVTTALAEALFRAGELAESE
                     ALLVPIVERPDAPARAQLTLGRLYDARGRSADAVDRMDRAVAAADDDPWVMYWSADAT
                     RSRAEAIERLERYVELAGPDDEDRIEAAEGSLRLYRALGETPIWVDENRPERAELPLR
                     QLWDPTTSATIGYVIEARIGDKDKPVKLLLDTGSPGLYVIDRIADKRGFRELSLQTTF
                     GGGGDRRHATRRGFFDSFAVGELSFRSALATAAKHEIDPLGRFHGVVGISIFNGYRVT
                     LDLAAERLVLAPIDEEPAAGAPYWVVGGQWLVEASIVPDGRSGLFLLDTGATRSLLSL
                     ALLDGLAAAVVRQGAEVHGFGGMIDGAREVDGVRLDFHGLGVGPGGLRAADLSLQSKM
                     GGVELSGFVGLDLLEGPVITIDTERRTVRVEANDDSRR"
     gene            complement(2947..3831)
                     /locus_tag="VD788_06875"
     CDS             complement(2947..3831)
                     /locus_tag="VD788_06875"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXV76031.1"
                     /translation="MSYTESIDTAVWRSRDRAGCEELWLVLDAAGIPARVVRGGGGWS
                     LAVAAASASAARREIDEYRNEAERRSPPPVGSLPGGAAGAAAYVAVLIGTAALARRGA
                     FGAEWLTAGRLDAGLVVEGEWWRTVTALTLHGDAAHLVANLAFGVVFGLLLAQALGGG
                     LTWLAVLVGGAAGNALDGWLQPARHAAIGASTAVFAALGLLLALSFPRRDPAPSGKMR
                     LLSPLIAGFLLLVYTGMGGERTDVLAHLTGLVCGALVGLPLRRIPRDRLARSRVQLAS
                     ASLAALFVLAAWYVALAA"
     gene            3898..4764
                     /locus_tag="VD788_06880"
     CDS             3898..4764
                     /locus_tag="VD788_06880"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HXV76032.1"
                     /translation="MLLHLHRPGPPLDGFVELVTYFRDFCPSHTKEKLLPDGAVEIIV
                     DLTDRPKKMFDRDDLSRGRAFERAWISGMRREWIVIEAQRGAAMVVIRFRPGGALPFL
                     GFAVESITGAVEPLDDVLGVVTASLRDRLLEARTTAGKMAAVEGWLLDRAAGRLERNP
                     VVEFLADRLFAPAGIRISNLVAEIGYSQRHVLALFRKWVGLTPKQYGRIRRFQQVLAD
                     VSHGANGPDAMAAYPSPLTRLTEPEWVDLALRHGYYDQAHLIRDFNEFAGLSPGAYVA
                     AYRGLENYLPLD"
     gene            4851..5258
                     /locus_tag="VD788_06885"
     CDS             4851..5258
                     /locus_tag="VD788_06885"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HXV76033.1"
                     /translation="MGELQINVWAVLAAAAANFVIGGLWYSPALMGRVWMRANGFSEA
                     DLAKGSPSVIFGLSFVFCLLMSANLAAFLGDPSVTVGFAVAAGIAAGAGWAALGLGVI
                     ALFERRPFSYILVNGGYLTLSFAAMGAILGAWR"
     gene            5352..5942
                     /locus_tag="VD788_06890"
     CDS             5352..5942
                     /locus_tag="VD788_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76034.1"
                     /translation="MKQTAWRVALATFLVLAVWPVAAESRRPPVDVGPAFDLLDPTGE
                     VLATVDEEIAGRMIARLKRETRRTEGLPEATFEALAIASSELRALDSAGALETESRPA
                     IGALAMIAVVDALVEHFPEIEGRLAAQTRSDEPSPIEKICTCDRSGSRACGCLVVSTG
                     GGGCQYRVSCGTWGSATCSAVNFEMCIAREVIGIVW"
     gene            complement(6001..>6447)
                     /locus_tag="VD788_06895"
     CDS             complement(6001..>6447)
                     /locus_tag="VD788_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76035.1"
                     /translation="RDHDEVYYAGCPVSADGLPLRRHGDRLERLEGGYYRSQGRADDT
                     MNLGGVKVGSLEIEQVLERHEAVAEAAAVGVQLGGEGAEQLVAYVVLRAAVDRSRLGA
                     ELACAISRELNPLFRLHDVVVVDTLPRTASNKLMRRALRACYPTGA"
BASE COUNT          918 a         2234 c         2303 g          992 t
ORIGIN      
        1 gccaagctct acgtcgaacg atgagccacc gtcggggcct cccttccgcc ggcgcacgcg
       61 ctgcgctaga ttagcggcat gcgatgcccg cgctgtcaga ccgtcctcga gcggcgcgac
      121 cacgacggcg ttcgcgtcga cacctgcgca acgtgcgcgg gagtattcct gcaccgcggc
      181 gagctgaacc ggatcgcgcc gacggagggc gatctcgagt tctcgacgct gcacgaggag
      241 tcgttccggc acgaggacga attcggtccg attgcctgtc cggcctgctc cggcccgacg
      301 atgaacaagg tcgagttcaa cgtctacagc ggcatcatgc tcgactattg cccggcgtgc
      361 cgcggtttct ggctggacgg caaggagctc gaccggatcg tcgaggaggt gcgtgggctc
      421 gaccggccgg aggacgacgt gggggccggt ccgctgcagt ggttcgccca gttcctgtgg
      481 agtcttccgc gctagatctg cggttgcaac gccgctccca cggcctagat ttcgtctccg
      541 acagggccag gggaatcatc aggccgattc caggtaggtc atggtcacgg caaacgaccc
      601 acgggacttg gcggcccgga tcgtcgaatc ctccgcggcc gccatcgtcg tcaccgacgc
      661 cggggcccgg atccggttcg tcaaccccgc tttcacccgc atcaccggct acccacccga
      721 cgaggtgctg ggccgcagtc cgcggctgct ccagtcgggt cggcattcgc cgaagttcta
      781 ccagcgaatg tggcgccggc tgacgcaccg gcggcgttgg cacggtgaga tctgggatcg
      841 gcgcaagaat ggcgaggtct ttccgatgcg gatgtcggtc accgcgatcg acgagcgcgg
      901 cgctacgctg tacgtcggcg tattcgagga gatcgccgca gagaagcgcc gggaggagcg
      961 cctctgccgc ctggcgttct acgacccgct gaccgggctg cccaaccgga cgctgctcgc
     1021 cgaccggctc gagcgcgggc tggtgcaggc caagcgcagc gggcgctgtc tcgcggtgct
     1081 gttcgtcgac gtcgacgggt tcaagcacgt caacgaccgc ttcggtcacg cgatcggcga
     1141 tcgggtgctg gcgcgggtcg gtcggacgct gaccctcggt ctgcggcgga gcgacacggt
     1201 ggcgcgcgtc ggcggcgacg agttcgtcgt tctgctcccc gatctcgact cgcgacggca
     1261 ggcgatcccg gtcgcgcgca agatcctgcg ctcgttatcg gccggcctga ccgtgggcgg
     1321 ccgtgaattg gctgtcggcg ccagcatcgg catcagcagc tatccggacg acgcgcggga
     1381 cgcccgacaa ctgcttcgtc tggccgactc ggcgatgtac cggctcaagc gggagcggag
     1441 gggagcgcgg ccccgaccgc ggccgtcacg cggcgtgcta cagtcggacg gatgatccga
     1501 cgggcgtcag cttcggtcgc agtcgcgctg tggatcgtcg acgcggcgct cggaccggca
     1561 gccgggcagt cgccggccgc gcccgacccg gcgtcgtcgt tcgccgagcg tctcgagcgg
     1621 aacccgctgg atcgccaggc gatggtcggg cgggtcgagg cgctgttggc gtcggatcgc
     1681 tggcggacgg cgctcgagga agcacgcgcg tattccgcgg tcgctcccga cgaccggcac
     1741 gtcacgacgg cattggccga ggccctgttc cgcgccggcg agttggcgga gagcgaagcg
     1801 ctgctcgtcc cgatcgtgga gcggccagac gcgccggccc gcgcccaact cacgctcggc
     1861 cggctgtacg atgcccgcgg gcgctcggcc gacgcggtcg atcggatgga tcgcgccgtc
     1921 gcagccgcgg acgacgaccc gtgggtgatg tactggtcgg ccgacgcgac gcggtcgcgc
     1981 gcggaggcga tcgagcgcct cgagcgctac gtcgagctcg ccggcccgga cgacgaggat
     2041 cgcatcgaag cggccgaggg cagcctgcgg ctctaccggg cgctgggcga gacgccgatc
     2101 tgggtcgacg agaatcgacc ggagcgggcg gagctgccgt tgcgccagct gtgggacccg
     2161 acgacctcgg cgacgatcgg ctacgtcatc gaggcgcgca tcggtgacaa ggacaaaccg
     2221 gtcaagctgc tgctcgatac cgggagcccg ggcctgtacg tcatcgaccg gatcgccgac
     2281 aaacgcgggt tccgcgagct gtcgctccag acgacgttcg gcggcggcgg cgaccgccgg
     2341 cacgcgacgc ggcgtggttt cttcgactcg ttcgccgtcg gggaattgag cttccggtcg
     2401 gcgttggcca ccgcggccaa gcacgagatc gacccgctgg ggcgctttca cggcgtcgtc
     2461 gggatctcga tcttcaacgg ctatcgcgtc acgctcgacc tggcggccga gcggctggtc
     2521 ctcgcgccga tcgatgagga gccggccgca ggcgccccgt actgggtcgt cggcggtcag
     2581 tggctggtcg aggcgagcat cgtgccggac ggtcgcagcg ggctgttcct gctcgacacc
     2641 ggcgcgacgc gttcgctgct gtcgctggcg ttgctcgacg gtctggccgc ggcggtcgtc
     2701 agacagggag ccgaggtgca cggcttcggc gggatgatcg acggcgcgcg cgaggtcgac
     2761 ggcgtgcgac tcgactttca cggcttgggc gtcggtcccg gtggtctgcg tgcggcggac
     2821 ctgtcgctgc agagcaaaat gggcggcgtc gaattgtcgg gtttcgtcgg tctcgacttg
     2881 ctcgaggggc cggtgatcac catcgacacc gagcgccgca cggtacgggt cgaggcgaac
     2941 gacgactcac gccgctagcg cgacgtacca cgcggcgagg acgaacagag ccgcgagcga
     3001 ggcggacgcg agctgcaccc gcgatcgggc caaccgatct cgcgggatgc ggcgcagcgg
     3061 aaggccgacg agcgcgccgc agaccagacc ggtgaggtgc gcgagcacgt cggttcgctc
     3121 accgcccata ccggtgtaga ccagcagcag gaaacccgcg atcagcgggc tgagcagtcg
     3181 catcttgccg ctcggcgcgg ggtcgcgccg agggaaggac agcgcgagga gcagacccag
     3241 cgcggcgaac accgcggtcg acgcgccgat cgcggcgtgg cgcgccggtt gcagccagcc
     3301 gtcgagggcg ttgcccgccg cgcccccgac cagaacggcg agccaggtca ggccgccgcc
     3361 gagcgcctgt gcgagcagca acccgaagac gacgccgaac gcgaggttgg cgacgagatg
     3421 cgccgcgtcg ccgtgcaacg tcaatgcggt gaccgtgcgc caccactcgc cttcgacgac
     3481 gagaccggcg tcgagacgtc ccgccgtcag ccattccgca ccgaaggcac cccgccgcgc
     3541 cagcgccgcg gtcccgatca ggacggcgac gtaggccgcg gcgcccgcgg cgccacccgg
     3601 caggctgccg accggcggcg gcgagcggcg ctcggcttcg ttgcggtact cgtcgatctc
     3661 ccgccgtgcg gcgctcgccg acgccgcagc gacggccagc gaccatccgc cacccccgcg
     3721 cacgacgcgc gccgggatgc ccgccgcgtc gagtaccagc cacagctcct cgcagcccgc
     3781 gcgatccctc gatctccaga cggcggtatc gatcgactcc gtgtacgaca tcttctccac
     3841 catcgtaacc cgtgccgggc gccgaaccgc gccgccggtt cggcgtatac tcacggcgtg
     3901 ctgctccacc tgcaccgccc cgggccgccg ctcgacgggt tcgtcgaact ggtgacctac
     3961 tttcgggatt tctgtccgtc gcacacgaag gagaagctgc tgcccgacgg cgccgtcgag
     4021 atcatcgtcg acctgaccga tcgcccgaag aagatgttcg atcgcgacga tttaagccgc
     4081 ggtcgcgcgt tcgagcgggc ctggatatcc ggcatgcgcc gcgagtggat cgtgatcgag
     4141 gcgcagcgcg gcgcagcgat ggtcgtcatc cggttccgtc ccggcggtgc actgcccttt
     4201 ctcggcttcg ccgtcgagtc gatcacgggc gccgtcgaac cgctcgacga cgtgctcggc
     4261 gtggtgacgg cgtcgctgcg cgaccgcctg ctcgaggctc gaacgaccgc gggcaagatg
     4321 gcggccgtgg agggctggtt gctcgatcgc gccgccggtc gcttggaacg caacccggtc
     4381 gtcgagttct tggcggaccg gttgttcgcg ccggcgggga tccgcatctc gaacctggtc
     4441 gccgagatcg gctacagcca gcggcacgtg ctcgcgctgt tccgcaagtg ggtcggcctg
     4501 acccccaagc agtacggcag gatccgacgc ttccagcagg tgctggccga cgtctcgcac
     4561 ggggccaacg gtcccgacgc gatggccgcc tacccgtcgc ccctcacccg gttgaccgag
     4621 cccgagtggg tcgatctggc gttgcgtcac ggctactacg accaagccca cctgatccgc
     4681 gacttcaacg agttcgccgg cctctccccc ggcgcctacg tcgccgccta ccgcggcctc
     4741 gaaaactacc tgccgctcga ctgatcgcgt catctccgtt ttatccaaga ccggcctgcc
     4801 gcgcgccgcc tatcctcccg accggtgatc caaccgagga ggaaatcgcg atgggagagt
     4861 tacagatcaa cgtctgggcg gtgctcgcgg cggcggcggc caacttcgtg atcggcgggc
     4921 tgtggtactc gccggcgctg atgggcaggg tgtggatgcg ggcgaacggc ttcagcgaag
     4981 ccgatctggc caaggggtcg ccctcggtga tcttcggcct gtccttcgtt ttctgcctgt
     5041 tgatgtcggc caacctcgcg gcgttcctgg gcgacccgtc ggtcaccgtc ggcttcgcgg
     5101 tggccgcggg catcgccgcg ggagccgggt gggccgcgct cggtctgggt gtgatcgcgt
     5161 tgttcgagcg gcgtccgttc agctacatcc tcgtcaacgg aggctacctg acgctctcgt
     5221 tcgccgcgat gggcgcgatc ctcggcgcgt ggcgctgatt gcgcgacgga aactccgcgg
     5281 gtccggccgg cgggcatccg gcgagtgcgg cgacgatctt gtcggtaccc acagcctccg
     5341 cggagttacc catgaaacag acagcttggc gcgtcgccct cgcgacgttc ctggtgctcg
     5401 cggtgtggcc ggtcgccgcc gagtcgagac gcccgccggt cgacgtcggc ccggccttcg
     5461 acctcctcga cccgacgggc gaggtgctgg ccaccgtcga tgaggagatc gccggccgga
     5521 tgatcgcgcg actcaagcgg gagacgcgtc gaaccgaagg gctgcccgag gccacgttcg
     5581 aagcgctcgc gatcgcctcg tccgagttgc gggcgctcga ctcggccggc gcgctcgaga
     5641 cggagagccg tccggcgatc ggcgcgctgg cgatgatcgc ggtcgtcgat gcgctcgtcg
     5701 agcactttcc cgagatcgag gggcggctcg ccgcccagac ccggtcggac gagccgtcgc
     5761 cgatcgagaa gatctgcacc tgcgaccgaa gcggctcgcg cgcctgcggc tgcctcgtcg
     5821 tcagtaccgg gggcgggggg tgtcagtacc gcgtctcctg cgggacctgg ggctcggcga
     5881 cctgctccgc ggtcaatttc gagatgtgca tcgcccggga ggtgatcggc atcgtctggt
     5941 agctcgctcg acgcgcggtc gtcgtggctc cgcctgcggg tcgcgaggac cgcgcgcgtt
     6001 ctacgcgccg gtcgggtagc acgcgcgaag cgcgcgtcgc atcagcttgt tcgacgccgt
     6061 gcgcggcagc gtgtcgacga cgacgacgtc gtggagccgg aagagcggat tgagctcccg
     6121 cgagatcgcg caggcgagct cggcgccgag ccgcgacctg tcgaccgcgg ccctcaacac
     6181 gacgtaggcg acgagctgct cggcgccttc accgccgagt tgcacgccga cggctgccgc
     6241 ctcggccacc gcctcgtgtc gttcgagcac ctgctcgatc tcgagcgaac cgaccttgac
     6301 gccacccagg ttcatcgtgt cgtccgctcg gccctgggag cgatagtagc cgccctccaa
     6361 ccgctccagg cggtcgccgt gtcgtcggag cgggaggccg tccgcggaca ccgggcaacc
     6421 ggcgtagtag acctcgtcat gatcgcg
//
LOCUS       DASPGN010000238        15286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1095084, whole genome shotgun sequence.
ACCESSION   DASPGN010000238 DASPGN010000000
VERSION     DASPGN010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 15286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15286
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1095084"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VD788_06900"
     CDS             complement(<1..590)
                     /locus_tag="VD788_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV76036.1"
                     /translation="MRGVSRRVPAASGDARLLDGVDLALGRGEVLHIVGPSGAGKSSL
                     IRLINRLDEPSGGRIEVLGRPLADWRVRELRRRVAMVFQESTLLGMSVRDNLTLPFEL
                     AREVPDDLDRRIDGWLERTELDREVLERDATALSVGQKQRVALARALIGEPEMLLMDE
                     PTSGLDPRTAARLLDRVSELCRERRLTLVMVTHRLE"
     gene            complement(620..2026)
                     /locus_tag="VD788_06905"
     CDS             complement(620..2026)
                     /locus_tag="VD788_06905"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HXV76037.1"
                     /translation="MREFIAAVTRNPVSLLGTAITTASALVFCGLFAIELTSRHENPY
                     LGILSYMVLPGLFVVGLVLIPIGAARARKKARAAAARGEKFQDFPIIDLNVGRMRRIV
                     LTFLVLTLANLVLLATATYKGVEYMDTTEFCGTVCHTVMHPEFTAYERSPHARVKCVE
                     CHIGPGANWFVRSKLSGAWQVVSVTFDLFPRPIPSPIENLRPARDTCEQCHWPAKFVG
                     DKLQVRTRFQEDEANTETKTVLLMRVGGVEGRVAHGIHWHVDPSRTIRYLADPTREEI
                     YDVELTEPDGTVKRYNSGEPPADREVRWREMDCVDCHNRPTHVYRKASTELDRALSDG
                     RIDRELPFIRRQGRLVLEQDYASHAAAREAISAALASYYRENHPEIAAVKTDRIAQAA
                     NEIGNIYGWNVFPEMNVEWGTYPNHIGHEDSPGCFRCHDDLHETEDGEAISQDCETCH
                     TLLALEEEDPEVLTMLQP"
     gene            2195..3214
                     /locus_tag="VD788_06910"
     CDS             2195..3214
                     /locus_tag="VD788_06910"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HXV76038.1"
                     /translation="MQPIRVALMGFGRIGRNLFRILYRRDDVQIAAISDVADHSSLEY
                     LLRFDTILGRFADAVSIKDGNLYVVGKQIPMLSGKGPRDVPWTDLDVDVVVEATAKPR
                     TRADFDDYLERGVGRVILCAPPADPPDITVVIGVNDHKLTPAHRIVSGASCTANCVAP
                     LIKILDDAFGIERSFLTTVHAYTNQQRLADVPSDDPRRGRAAAENIIPQETNAAEVVM
                     DLLPRLRGRVSAMAINVPVPNGSVVDLVCWHEKAVDVIAINEVVRTAAASRYNGILNY
                     EDAPIVSSDIVHMPHSGTFDALATMVLDKRVSKTLTWYDNGWGYAHRVVDLIHKFAAM
                     EREAA"
     gene            3214..4215
                     /gene="gap"
                     /locus_tag="VD788_06915"
     CDS             3214..4215
                     /gene="gap"
                     /locus_tag="VD788_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HXV76039.1"
                     /translation="MATTVGINGFGRIGRGVFRILSKRDDIRVVAINDLYDNEQLAYL
                     LRYDTVMGRFNRKIRVDGDTLFVDDRPVVMTAHRDPAEIPWSKLGVAVVVEATGVFRS
                     REQLERHLDAGADKVVLTVPAKDEIDATIVIGVNDEVLEPQHRIVSNASCTTNCLAPI
                     AKILDDAFGLEQGFITTVHAYTNDQRLADVPHKDPRRSRAAAENIIPTTTGAARAVGK
                     VLPRLAGKLDGMAMRVPVPNGSIVDLVCRLRRNVDRTQVNSAVKAAADGPMADIVEYS
                     EDPLVSSDIIGNPRSSIFDASSTMADGDEFIKVVSWYDNEWGYANRVVDLIERMSGT"
     gene            4254..4778
                     /locus_tag="VD788_06920"
     CDS             4254..4778
                     /locus_tag="VD788_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HXV76040.1"
                     /translation="MSPHPAGRGLTVLVAACAWCAGLAGDVEVEVGRDADLSAYATYS
                     WKGGEPPADDWARLTIVEAVDRELASRGLSKVATGGDLSVSALALAESVTGTTVDPGY
                     WGGGTDFGMPSVDIADYRKGSLYVTVEDRAAGRVVWKASGKANIEMGTQDKFADKIES
                     VVSRVFRKFPRRAR"
     gene            4775..5308
                     /locus_tag="VD788_06925"
     CDS             4775..5308
                     /locus_tag="VD788_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HXV76041.1"
                     /translation="MRGSLLVACCVWLATTGVDAAGRFYLGGGAGSSSAELSANTRVS
                     FDATGYKGYFGYEATRFLAVEASYTDFGDLDETVNGQRFQGDASTAALWAVGLLPVTP
                     RIGLFGRLGYSAYDTEITVEGSSEPTKTDGNDLAWGFGLWYRFTRSWALRLEWESYRF
                     EESDDVTLTSLSVAFKF"
     gene            complement(5298..6020)
                     /locus_tag="VD788_06930"
     CDS             complement(5298..6020)
                     /locus_tag="VD788_06930"
                     /inference="COORDINATES: protein motif:HMM:NF018084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1054 family protein"
                     /protein_id="HXV76042.1"
                     /translation="MPLLFEQPPRFEGLPVSGFDAFAIEQRELRRRSIIDSFHPPLKL
                     LGEDLVARLEPSDDGAPGVEAPLHIHLPRLDWPRGYEPFCTWLALSREAHGYQAGPQL
                     NVGVHRDYVAVRLGWDVAADGFGRFEFLCRRGDLGQALVGVADEQRLRFRVYASAPMP
                     VGSRCVFDSARDVDASFRQLTTSGVWWELGHRFDLPQATDVVCSPELVNETSRIFRAL
                     LPLYDRIVGEHHERRDDDPLRT"
     gene            6090..6821
                     /locus_tag="VD788_06935"
     CDS             6090..6821
                     /locus_tag="VD788_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76043.1"
                     /translation="MSRWAIVASAAVLAALATTAAEGSDAEQRWRAAAGRMLGGTSQL
                     RSLKLELTVAPRADGSADEVRQVVEIELPGRVRRAIFVGGEEQLVVIDGARGYFGDAG
                     RVLPLPEPQLRQSLDQLRRDLLVLAANAANDRLTVVDGGQHTIGGLDCTALDVSLDGV
                     RSRLFVTPTGEVIRQTFGGSDPLSGKPGLIQVDYSAYRELDGVRFPARYDVYLDGRPV
                     VSVRVDSLLVNPPLDPARFAVPVSD"
     gene            6821..7573
                     /locus_tag="VD788_06940"
     CDS             6821..7573
                     /locus_tag="VD788_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXV76044.1"
                     /translation="MPGPRARYPVVLLDVGGTIVGPETSFGSIYASVLSGLGLTVDDA
                     EIERSIHETWREMNRVIPPGSDRYRHFPGGEDEYWLRFTRATLTHALGRPPVDGVAAP
                     ALAGIRRAFLEPGAWRVFADVRPALARLRAAGIRLAVVSNWDSNLPRVLGMLGLAPYF
                     EAVVISHVEGVEKPHPELFRRALARIGATADQALHVGDVPELDLAGAAAAGIDAVLVD
                     RHRRFDATLRPIPDLSALPRIVRHGLAREPRG"
     gene            complement(7570..8649)
                     /locus_tag="VD788_06945"
     CDS             complement(7570..8649)
                     /locus_tag="VD788_06945"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HXV76045.1"
                     /translation="MRFESLAAKPVLLGLIVLAVGVACTSGPAEAQAPPAKPAGEPAP
                     TVFGENFVAGSFDNVNRQAGGPAAIDLDAALGAKPVVLYYWIAGNTRADKVFVELQEL
                     VFDTGRDKLDLFGVVFKRTERDEQAIADRVVSLGIRVPVLNDTDFALGKRLQVRSVPN
                     VTIFDAEGRLRLTNGASLLQQLEYNIDLAGAVRRVAETGSLGTYGYLEAYHPVEELVG
                     QKCPDFEAPLLDGDTAKRWSNLLADDKVNVLIFWSVDCPHCRQSLPEINEWLKANGEG
                     VNVISAAKVTNEATRIKTREFCEYNGFVFPTLVDQDLQIADLYSVTSTPTILFIRPDG
                     VIDSVATSAGHDFGKLVEAKKKSML"
     gene            complement(8713..9327)
                     /locus_tag="VD788_06950"
     CDS             complement(8713..9327)
                     /locus_tag="VD788_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXV76046.1"
                     /translation="MDDDRAVAAIVLAAGGARRFGSSKALARLGGRELIRIAVDAAIG
                     GGCRPIVVVLGAEAAAVARALDGLSVLRVIHRGWRRGVASSIRAGLAEVERSNPHAPA
                     VLLVGIDQPLIDAELVGRLLAASEGGSRPAAAAYADTIGIPAVVPRERFAALRRLTGE
                     GGAKRTLLAAGPRLARVDWPDGAHDVDRREDLARLAPTRRRPGR"
     gene            complement(9330..10268)
                     /gene="hflC"
                     /locus_tag="VD788_06955"
     CDS             complement(9330..10268)
                     /gene="hflC"
                     /locus_tag="VD788_06955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HXV76047.1"
                     /translation="MKAAVWMPVAVGVLLLLAALGGAFFVVNETQQVVITQFGEPKGD
                     PITDPGLNVKLPFIQKAHYFDKRFLEWDGDPNQLPTKDKRFIFVDTYARWRISDPLKF
                     FQRLRDERGAQSRLDDILDGETRNTIASHNLIEVVRSSDREFAVADDLRDASADETAI
                     VVELGKEALEREVLANASARTSDLGIEILDFRLKRINYVQEVRERVYDRMISERKRIA
                     ERYRSEGAGEAARISGEKELELKRITSEAYREAQEIRGVADARAADIYAAAYNRDPEF
                     YRFFKTMETFGNAFDEETVLVLGTDGEYLRYLERSD"
     gene            complement(10265..11260)
                     /gene="hflK"
                     /locus_tag="VD788_06960"
     CDS             complement(10265..11260)
                     /gene="hflK"
                     /locus_tag="VD788_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HXV76048.1"
                     /translation="MAARDIRSVPPQQFRPPSVNFRLLALILAGMVVGALLYDMIYQI
                     EPEAVGVVLTFGKFSQTTEPGLHLKLPWPIQSVTEVAVQRQLKQEFGFRTEEPGVRTR
                     YAQGTFEGESLMLTGDLNVAVVEWTAQYRVDDPYRYLFRVRSITETFRHMNEAVMRAV
                     VGDRSVTEVLTIGRQEIATEAERRLQELCDQYESGIHVEQIVLQDVNPPEGVRDSFNE
                     VNESQQERERMINDARADFNRVIPKARGEAAQMLKIAEGYAADRVNRARGDAALFDSL
                     RAAYARAPEVTRRRIYLETMETLLPKVKRKIVLDDDLEGLLPLLPLGGAEKGGQP"
     gene            11389..12063
                     /locus_tag="VD788_06965"
     CDS             11389..12063
                     /locus_tag="VD788_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76049.1"
                     /translation="MRRALARRGGGIVLSVLCAAPVLAGGLDDVARLFERNRQQLRDY
                     VWTSTTEIALEGERPTVERYEVRVDENGVQRRTTVPSAAGRGRKAGKRQRQAEELHDS
                     LRSLVESYVHPAPETTRTLFADPFVWRGEGRIEGVTRLQARNVVREGDQVSLWLETAT
                     ERPTKLEILTSSGGEPVRVTTEFARIDQGPFYPARIVMETEIKEKRLVVTTANADYRR
                     AERASP"
     gene            12126..12824
                     /locus_tag="VD788_06970"
     CDS             12126..12824
                     /locus_tag="VD788_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HXV76050.1"
                     /translation="MELVRARRGPAAAVFALLGLVVAGPPASGEPAEPPAGVVHVVER
                     GQTLWRIARTYEVEIGELARINGIADPTRIHTGQRVFVPGVDSLRVVVPPSASPVLAA
                     SAWAWPVEEGVLLSYFGAPRRSRDHQGVDIRARHGQPVVAARAGRVVYSGAGMRGYGK
                     TVIIDHGDGISTLYAHNSALAVRVGQRVAQGERIARVGRSGNASSDHCHFEIRHQDRP
                     VDPLPYLARSEARR"
     gene            12821..13582
                     /locus_tag="VD788_06975"
     CDS             12821..13582
                     /locus_tag="VD788_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV76051.1"
                     /translation="MNVEVHCTDCRTAYVLEGGFAGTTLPCPACGAPDGLTVPADAPE
                     PPFRIARPVAAAVTAAHSSAPSVVAETRPVPTPPAASTGPSPATVRAKPSERAAQRSN
                     EADEVVCPRCKLHFVPRKGQLETGNSGRRSVLVVEAMDYFREIAAEALADHFAVKQAA
                     DVGEARALLDAEEFDLLVLDPESGGIELLRGLGRKPCPILVYTDRDESEIYGDSWDEL
                     QRYGADDIVMKAMNAGETLARKASVLLGVRIDEDE"
     gene            complement(13921..14553)
                     /locus_tag="VD788_06980"
     CDS             complement(13921..14553)
                     /locus_tag="VD788_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXV76052.1"
                     /translation="MRRRTMLMLAMLVAVASVASTGCVSKKMFRKNVEANDTRVAAVE
                     SGVEANERRIKDLSEETDSKIAELDAKTERATEVGQSAMTVAEKAVRGRLIWEVTLNN
                     DQVKFSFNQADVPQEAARVLDDLVGKIMSYDKAVYVEVEGHTDATGTDAYNYDLGYKR
                     AMTVRDYMAKNGVPLHAINVISLGEEKPVANNDSTEGRAQNRRVVIRVLE"
     gene            complement(14669..>15286)
                     /locus_tag="VD788_06985"
     CDS             complement(14669..>15286)
                     /locus_tag="VD788_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV76053.1"
                     /translation="AKQAIRVFADGLRPDDRLGLVCFADEQIDWVTELTRDRRRFLER
                     LAVQHGYGQTALLDALAAAPGLLDAEEAGSKAIVLLTDGIDTASKQNLWNALTLARSV
                     NVPIYAIGFSTFASRLLPSGSTPEEHRIVKLFADETGGRLFVVRDPDDLKEATLAVQR
                     ELRFRYVISYRPERTLWDGSYRRIQVETANDRLVVRARRGYYAEP"
BASE COUNT         2366 a         5223 c         5225 g         2472 t
ORIGIN      
        1 tcctccagcc ggtgcgtcac catcaccagc gtcaaccgcc gctcgcgaca caactcgctg
       61 acgcggtcga gcagccgcgc ggcggtgcgc gggtcgagac ccgaggtcgg ctcgtccatc
      121 agcagcatct cgggttcgcc gatcaacgcg cgggccagcg ccacccgctg cttctggccg
      181 acgctgagcg cggtcgcgtc gcgctcgagc acctcgcgat ccagctccgt ccgctcgagc
      241 cagccgtcga tccgccggtc gagatcgtcc ggcacctcgc gcgccagttc gaacggcaac
      301 gtcaagttgt cgcgcaccga catcccgagc agcgtgctct cctggaacac catcgccacc
      361 cgacggcgca gctcgcgcac gcgccagtcg gccagcggcc ggccgagcac ttcgatccga
      421 cctccgctcg gctcgtccag ccggttgatc agccggatca agctgctctt gccggcaccg
      481 ctcggtccga cgatgtgcag cacttcgccg cggccgagcg cgaggtcgac cccgtccaac
      541 agccgtgcgt cgccgctcgc ggccggtacg cggcgcgaca cgccacgcat ggtgaaggcc
      601 gggtcgttgg gccgggcggt cagggctgga gcatcgtgag aacctcgggg tcctcttctt
      661 ccagggccaa cagggtatgg caggtctcgc agtcctgcga gatcgcctcg ccgtcctcgg
      721 tctcgtgcag gtcgtcgtga cagcggaaac agcccggcga atcttcgtgg ccgatgtgat
      781 tcgggtaggt gccccattcc acgttcatct ccggaaagac gttccagccg tagatgttgc
      841 cgatctcgtt cgccgcctgc gcgatgcggt cggtcttgac cgccgcgatc tccgggtggt
      901 tctcgcgata gtagctcgcc agcgcggccg agatcgcctc gcgcgccgcc gcgtgcgagg
      961 cgtagtcctg ctcgagcacc aagcgcccct gccggcggat gaacggcagc tcgcggtcga
     1021 tccgtccgtc ggacagcgcg cggtccagct cggtcgacgc cttgcggtag acgtgcgtcg
     1081 ggcggttgtg gcagtcgacg cagtccatct cgcgccagcg cacctcccga tccgcgggcg
     1141 gctcaccgct gttgtagcgc ttgaccgttc catccggctc ggtcagctcg acgtcgtaga
     1201 tctcctcgcg cgtcgggtcg gccaggtagc ggatcgtgcg gctcgggtcg acgtgccagt
     1261 ggatgccgtg ggcgacgcgt ccttcgacac cgccgacccg catcaacagc accgtcttgg
     1321 tctcggtgtt ggcctcgtcc tcctggaagc gcgtccggac ctgcagcttg tcgccgacga
     1381 acttggccgg ccagtggcat tgctcgcagg tgtcgcgcgc cgggcgtagg ttctcgatcg
     1441 gcgacgggat cggccgcggg aacaggtcga acgttaccga caccacctgc cacgcgcccg
     1501 acagcttgga ccggacgaac cagttggcgc cgggaccgat gtggcattcg acgcacttga
     1561 cgcgggcgtg cggcgaacgt tcgtaggccg tgaactccgg gtgcatcacc gtgtggcaga
     1621 ccgtgccgca gaactcggtc gtgtccatgt actccacgcc cttgtaggtc gcggtcgcca
     1681 acagaaccag gttggccagc gtcagcacga ggaaggtcag cacgatcctg cgcatgcggc
     1741 cgacgttgag gtcgatgatc gggaagtcct ggaacttctc gccgcgcgcg gccgccgcac
     1801 gggctttctt ccgtgcccgc gccgcaccga tcggtatcag gaccaggccg acgacgaaca
     1861 ggccggggag caccatgtac gagaggatgc cgaggtacgg attctcgtgg cggctcgtca
     1921 gctcgatggc gaacagaccg cagaagacga gggcgctggc ggtggtgatc gccgtgccga
     1981 gcagtgagac cgggttgcgg gtgacggccg cgatgaattc gcgcatgcac ggagcctccg
     2041 gcgaacggac gagggtggat tctagctcgt ttgcaacgag agttgaaccg aacagggtgc
     2101 aacgtcgtcg ccgagcctcg cccgcggtgc ggaatcgttc tagaatcgag ggaccgaacc
     2161 agcgaacgcg cgcccgactt cacggaggaa gcgaatgcag ccgatacgcg tggccctgat
     2221 gggcttcggc cggataggcc ggaacctctt tcgaatcctc taccggcgcg acgacgtgca
     2281 gatcgcggcg atcagcgacg tcgccgatca ctcgtcgctc gagtacctgt tgcggttcga
     2341 cacgatcctc gggcgcttcg ccgacgcggt gagcatcaag gacggcaatc tgtacgtcgt
     2401 cggcaagcag attccgatgc tgtcgggcaa gggaccgcgc gacgtcccgt ggaccgacct
     2461 cgacgtcgac gtcgtcgtcg aggcgacggc caagccgcgc acgcgcgccg atttcgacga
     2521 ctacctggaa cgcggggtcg gtcgggtcat cctctgtgcg ccgccggccg acccgccgga
     2581 catcaccgtc gtgatcggcg tcaacgacca caagctgacc cccgcccacc ggatcgtctc
     2641 gggcgcgtcg tgcaccgcca actgcgtcgc gccgctgatc aagatccttg acgacgcgtt
     2701 cggcatcgag cggtcgttcc tgacgacggt gcacgcctac accaaccagc agcgtctggc
     2761 ggacgtgccg tccgacgacc cgcgccgcgg ccgggccgcc gccgagaaca tcatcccgca
     2821 ggagaccaac gccgccgagg tggtgatgga cctgctgcca cggctgcgcg gtcgcgtctc
     2881 ggcgatggcg atcaacgtgc ccgtgccgaa cgggtcggtg gtcgacctgg tctgctggca
     2941 cgagaaggcg gtcgacgtga tcgcgatcaa cgaggtcgtg cgcaccgcgg cggcaagccg
     3001 ctacaacggg atcctgaact acgaagacgc cccgatcgtc tcgagcgaca tcgtgcacat
     3061 gccgcactcg ggcaccttcg acgcgctggc gacgatggtg ctggacaagc gcgtgtcgaa
     3121 gacgctcacc tggtacgaca acggctgggg ctacgcgcat cgcgtcgtcg acctgattca
     3181 caagttcgcc gcgatggaac gggaggccgc gtgatggcga cgaccgtcgg gatcaacgga
     3241 ttcggtcgga tcggccgcgg ggtgtttcgc atcctgagca agcgggacga catccgcgtg
     3301 gtcgcgatca acgacctgta cgacaacgag caactcgcct atctgctgcg gtacgacacc
     3361 gtgatggggc gcttcaatcg caagatccgc gtcgacggcg acacgctgtt cgtcgacgac
     3421 cggccggtgg tgatgaccgc gcaccgcgac ccggcggaga ttccgtggag caagctcggt
     3481 gtcgcggtcg tcgtcgaagc caccggcgtc tttcgcagtc gcgagcagct ggagcgacat
     3541 ctcgacgccg gcgccgacaa ggtggtgttg acggtgccgg ccaaagacga gatcgacgcg
     3601 acgatcgtga tcggcgtcaa cgacgaggtg ctcgaaccgc aacaccgaat cgtgtcgaac
     3661 gcgtcgtgca cgaccaactg tctggcgccg atcgccaaga ttctcgacga cgcgttcggc
     3721 ctggagcagg gcttcatcac cacggtgcac gcctacacca acgaccagcg cctggcggac
     3781 gtgccgcaca aggacccgcg gcgcagtcga gccgccgcgg agaacatcat cccgacgacg
     3841 accggcgccg cgcgcgcggt gggcaaggtg ctgccgcggc tggccggaaa gctcgacggc
     3901 atggcgatgc gggtcccggt tcccaacggg tcgatcgtcg atctcgtttg ccggctaagg
     3961 cgcaatgtcg atcggaccca ggtcaactcg gcggtcaagg cagcggccga cggtccgatg
     4021 gcggacatcg tcgagtacag cgaagatccg ctggtgtcga gtgacatcat cggcaatccg
     4081 cgctcgtcga tcttcgacgc cagctcgacg atggccgacg gcgacgagtt catcaaggtc
     4141 gtgtcgtggt acgacaacga gtggggctac gcgaaccggg tcgtcgacct gatcgagcgg
     4201 atgagcggga cctgagcggc acaccggtgg cagcccaacg acgacgatcg atcgtgtcgc
     4261 cgcatcccgc cggtcgcggt ctgaccgtgt tggtcgcggc gtgcgcatgg tgtgccggcc
     4321 tggccggcga cgtcgaagtc gaggtcggcc gcgacgccga cctgtcggcg tacgccacct
     4381 acagctggaa gggcggtgag ccgcccgccg acgactgggc gcggctgacg atcgtcgagg
     4441 ccgtcgatcg cgagctggcg tcgcgcggtc tgagcaaggt tgccacgggc ggcgacctgt
     4501 cggtgtcggc gctcgcgctg gcggagagcg tcaccgggac gacggtcgac cccggttact
     4561 ggggcggggg caccgatttc ggcatgccga gcgtcgacat cgccgattac cgcaaggggt
     4621 cgctgtacgt gacggtcgag gaccgtgccg ctgggcgagt ggtctggaag gcctcgggca
     4681 aggcgaacat cgagatgggc acacaggaca agttcgccga caagatcgag tcggtcgtga
     4741 gccgggtctt tcgcaagttc ccacggagag ctcgatgaga ggatcgctat tggtcgcctg
     4801 ctgtgtgtgg ctcgcgacga cgggtgtcga cgccgccggc cggttctatc tcggtggcgg
     4861 cgcaggcagc tcgagcgcgg agctgagcgc caatacccgc gtcagcttcg acgccaccgg
     4921 ctacaagggc tacttcggct acgaggcgac gcggttcctc gccgtcgagg cgagctacac
     4981 cgacttcggc gatctcgacg agacggtcaa cggccaacgg ttccagggtg acgcttcgac
     5041 ggccgcgctg tgggccgtcg gcctgctgcc ggtcacaccg cgaatcggtc tgttcggccg
     5101 gctggggtac tcggcgtacg acacggagat cacggtcgag gggtcgtccg agccgacgaa
     5161 gaccgacggg aacgacctcg cctggggctt cggcttgtgg tacaggttca cgcggagctg
     5221 ggcgctgcgt ctcgagtggg agagctatcg attcgaggaa tcggacgacg tgacgttgac
     5281 ctcgctgtcc gtggcgttca agttctgagc ggatcgtcgt cccgtcgctc gtgatgttcg
     5341 ccgacgatcc ggtcgtacag cggcaacaac gcgcggaaga tgcgcgacgt ctcgttcacc
     5401 aactccggcg agcagaccac gtcggtcgct tgcggcaggt cgaaacgatg ccccagctcc
     5461 caccacacgc cgctggtcgt cagttgccgg aacgaggcgt cgacgtcccg agcggaatcg
     5521 aagacgcagc gcgaacccac cggcatcggc gcgctggcgt agacacggaa ccgcaaccgc
     5581 tgctcgtcgg cgacgccgac cagcgcctgg cccagatctc cacggcggca gaggaactcg
     5641 aagcggccga agccgtcggc cgccacgtcc caaccgagac ggacggcgac gtagtcgcgg
     5701 tggacgccga cgttgagctg cggcccggcc tggtagccgt gggcttcgcg ggagagcgcg
     5761 agccacgtgc agaacggttc gtagccgcgt ggccagtcga gtcgcggaag gtggatgtgc
     5821 agcggggcct ccacgcccgg ggcgccgtcg tccgacggct cgagccgcgc caccaggtcc
     5881 tcgcccagca gcttcaacgg gggatggaac gaatcgatga tcgaccggcg gcgcagctcg
     5941 cgctgctcga tcgcgaaggc gtcgaagccg gagacgggaa gcccctcgaa tctcggcggc
     6001 tgctcgaaca gcaggggcaa gccgatctcc ctccctcgcc gtcgctcgaa cgacccgtgc
     6061 gccggaacct taacagaaag cgattcgcgg tgtcacgctg ggcgatcgtg gcgtcggcgg
     6121 ccgtgctcgc cgccttggcg acgaccgccg cggagggctc ggacgccgag caacgctggc
     6181 gcgccgcggc cggtcgcatg ctcggcggga cgtcgcagct acggtcgctg aagctcgagc
     6241 tgaccgtcgc cccgcgcgcc gacggcagcg cggacgaggt gcggcaggtg gtcgagatcg
     6301 agctacccgg tcgggtccgc cgcgcgatct tcgtgggtgg cgaggagcag ctcgtcgtca
     6361 tcgacggtgc ccgcggctac ttcggcgatg ccggccgcgt cctgccgcta cccgagccgc
     6421 agctgcgcca gtccctcgat cagctgcgcc gcgacctgtt ggtgctggcg gcgaatgccg
     6481 ccaacgaccg cttgaccgtc gtcgacggcg gccagcacac gatcggcggg ctcgactgca
     6541 ccgcgctcga cgtcagtctc gacggagtcc gctcgcggct gttcgtcacg cccacgggcg
     6601 aggtgatccg ccagacgttc ggcgggagcg acccgctgtc gggcaagccc gggctgatcc
     6661 aagtcgacta ctcggcctac cgcgagctcg acggcgtacg ctttccggcg cgatacgacg
     6721 tctacttgga cggccggccg gtggtgtcgg ttcgagtcga ctcgttgctc gtcaatccac
     6781 cactcgaccc ggcgcgcttc gccgtgcccg tcagcgactg atgcctgggc cgcgcgcgcg
     6841 ttacccggtc gtgctgctcg atgtcggcgg gacgatcgtc ggccccgaga cgtcgttcgg
     6901 ctcgatctac gcctcggtcc tgtcgggcct cggcttgacc gtcgacgacg cggagatcga
     6961 gcgatcgatc cacgagacct ggcgcgagat gaatcgcgtc atcccgccgg gcagcgatcg
     7021 gtaccgccac tttcccggcg gcgaggacga gtactggctg cgctttacgc gcgcgacgct
     7081 cacgcacgcg ctcgggcgcc cgccggtcga cggcgtcgcc gcgccggcgc tcgccggcat
     7141 ccgccgcgcg tttctcgaac ccggcgcgtg gcgggtcttc gccgacgtgc gcccggcgct
     7201 cgcgcgactg cgcgcggccg gcatccgtct ggccgtcgtg tcgaactggg actcgaacct
     7261 accgcgcgtg ctcggcatgc tggggctcgc accctacttc gaggcggtcg tgatctcgca
     7321 cgttgaaggc gtggagaagc cgcatcccga gctgttccgc cgcgcgctgg cacgaatcgg
     7381 cgccaccgcg gaccaagcgc tgcacgtcgg cgacgtcccc gagctcgacc tggccggggc
     7441 cgccgccgcc ggcatcgacg cggtgctggt cgaccgccac cggcgcttcg acgcgacact
     7501 ccggccgatt cccgacctgt cggcgttgcc gcgcatcgta cgtcacggtc tggcccgcga
     7561 gccgcgaggc tagagcatgc tcttcttctt ggcttcgacc agcttgccga agtcgtgacc
     7621 ggcgctggtc gccaccgagt cgatcacacc gtcggggcgg atgaacagaa tcgtcggcgt
     7681 cgacgtcacc gagtagagat cggcgatctg caggtcctga tcgacgagcg tcgggaagac
     7741 gaagccgttg tactcgcaga actcgcgggt cttgatccgc gtcgcctcgt tggtcacctt
     7801 ggcagccgag atgacgttga ccccttcgcc gttggccttc agccactcgt tgatctcggg
     7861 cagcgactgc ctgcagtgcg ggcaatcgac cgaccagaag atcaacacgt tcaccttgtc
     7921 gtcggcgagc aggttggacc agcgtttggc ggtgtcgccg tccaacagcg gcgcttcgaa
     7981 atcgggacat ttctgaccga ccagttcctc gaccggatgg taggcctcga gatacccgta
     8041 ggtgccgagc gaaccggtct cggcgacccg cctgacggcg ccggccaggt cgatgttgta
     8101 ctcgagctgt tgcagcaggc tcgcgccgtt ggtcaaccgc agccgccctt cggcatcgaa
     8161 tatcgtcacg ttgggcaccg accgcacctg caggcgtttg ccgagtgcga agtcggtgtc
     8221 gttgagcacc ggaacccgga tgccgagcga gacgacgcgg tcggcgatcg cctgctcgtc
     8281 gcgctccgtg cgcttgaaga cgacgccgaa caggtcgagc ttgtcgcggc ccgtgtcgaa
     8341 caccagttcc tggagttcga cgaaaacctt gtcggcgcgg gtgttccccg cgatccagta
     8401 gtagagcacc accggtttgg cgccgagggc cgcgtcgaga tcgatcgcgg ccggcccgcc
     8461 ggcctgccga ttgacgttgt cgaacgagcc cgcgacgaag ttctcgccga aaaccgtcgg
     8521 ggccggttcg ccggcgggct tggcgggtgg cgcttgggct tcggcggggc ccgaggtgca
     8581 ggccacgccg acggccagca cgatcaggcc gagcagcacc ggtttggccg cgagcgattc
     8641 gaagcgcatg atctcacccg acttgcccgc cggccggtgc cgatcgcacg gtgacgggct
     8701 gccaattata gttcagcgac ccggccgtcg ccgggtcggc gcgagccgcg cgaggtcctc
     8761 gcggcgatcg acgtcgtgcg cgccgtccgg ccagtcgacc cgcgccagac gcggtccggc
     8821 ggcgagcagc gttcgcttgg cgccgccctc gccggtcagc cgacgcagcg cggcgaaccg
     8881 ctcgcgcggg acgaccgccg ggatcccgat cgtgtcggca taggccgccg ccgccgggcg
     8941 cgacccgcct tccgaggccg cgagcagccg gccgacgagc tcggcgtcga tcagcggttg
     9001 gtcgatcccg accagcagca cggccggagc gtgcgggttg ctgcgttcga cctcggccag
     9061 accggctcga atcgacgatg ccacaccccg gcgccagccg cgatggatta cgcgcaggac
     9121 ggacagcccg tcgagcgcgc gcgcgacggc cgcggcctcg gcaccgagca cgacgacgat
     9181 cgggcggcac ccgccgccga tcgccgcgtc gaccgcgatg cggatcagct cgcgcccacc
     9241 gagccgtgcg agcgccttcg acgaaccgaa gcggcgcgcc ccgcccgcgg ccaacacgat
     9301 cgccgcaacg gcacgatcgt cgtccatcgc tagtccgacc gctccaggta gcgcaggtac
     9361 tcgccgtccg tgccgaggac cagcaccgtt tcctcgtcga acgcgttgcc gaacgtctcc
     9421 atcgtcttga agaagcggta gaactccgga tcgcggttgt acgcggcggc gtagatgtcg
     9481 gccgcgcgcg cgtcggccac gccgcggatt tcctgcgctt cgcggtaggc ctcggaggtg
     9541 atgcgcttca gctcgagttc cttctcgcca ctgatgcgcg ccgcctcgcc ggccccctcg
     9601 gagcggtatc gctcggcgat gcgtttgcgt tccgagatca tccggtcgta gacccgctcg
     9661 cgcacctcct gcacgtagtt gatccgcttg aggcggaaat cgaggatctc gatgccgagg
     9721 tcgctggtgc gggccgaggc gttggccagc acctcccgct cgagcgcctc cttgcccagt
     9781 tcgacgacga tcgccgtttc gtcggcgctc gcgtcgcgga ggtcgtcggc gacggcgaac
     9841 tcgcgatcgc tcgagcggac gacctcgatc aggttgtggc tggcgatcgt gttgcgcgtc
     9901 tcgccgtcga ggatgtcgtc cagtcgcgac tgcgcgccgc gctcgtcgcg cagccgctgg
     9961 aagaacttca acgggtccga gatccgccaa cgggcgtagg tgtcgacgaa gatgaagcgc
    10021 ttgtccttgg tcggcagttg gttgggatcg ccgtcccact cgaggaaccg cttgtcgaag
    10081 taatgggcct tctggatgaa cgggagcttg acgttgagcc cggggtcggt gatcggatcg
    10141 cccttcggtt cgccgaactg ggtgatcacg acctgctgcg tctcgttgac gacgaagaac
    10201 gccccgccga gcgccgcgag cagcaacaga acgccgaccg ccaccggcat ccataccgcc
    10261 gccttcatgg ctggcctccc ttttccgccc caccgagcgg caacagcggg agcagcccct
    10321 ccaggtcgtc gtccagcacg atcttccgtt tgaccttggg cagcagcgtc tccatcgttt
    10381 cgaggtagat gcggcgtcgc gtaacctcgg gagcccgggc gtaggccgct cgcagcgagt
    10441 cgaacagcgc cgcgtcgccg cgcgcgcggt tgacgcggtc ggcggcatag ccctcggcga
    10501 tcttcagcat ctgcgcggct tcgcccctgg ccttcggaat cacccgattg aagtcggcgc
    10561 gcgcatcgtt gatcatccgt tcccgctcct gctgggattc attgacttcg ttgaaggaat
    10621 cccgaacccc ctccggcgga ttgacgtcct gcagcacgat ctgctcgacg tggatgcccg
    10681 actcgtactg gtcgcacagc tcctgcagcc gtcgctcggc ctcggtcgcg atctcctggc
    10741 gaccgatcgt cagcacttcg gtgacgctgc gatcgccgac gaccgcccgc atcaccgcct
    10801 cgttcatgtg gcgaaaggtc tcggtgatgc tgcgcacgcg gaacaggtag cggtacgggt
    10861 cgtcgacgcg atactgcgcg gtccactcga cgacggccac gttgaggtcg ccggtcagca
    10921 tcaacgattc gccctcgaac gtgccctgcg cgtaacgcgt gcgcactccg ggctcctcgg
    10981 tacggaagcc gaactcctgc ttgagctgcc gttgcaccgc gacctccgtc accgattgga
    11041 tcggccacgg cagcttgagg tgtaggccgg gttcggtggt ttgcgagaat ttgccgaagg
    11101 tcagcacgac gccgacggcc tccggctcga tctggtagat catgtcgtac agcagcgcgc
    11161 cgaccaccat cccggccagg atcagcgcga ggagccggaa gttgaccgac ggcggtcgaa
    11221 actgctgcgg cggcacactg cgaatgtcac gagcggccat cgagccctcc ctcgtttggg
    11281 aacggatctg ttctcgtcgg ctccgagtct atcagggtgc ccggcgaccg gtcggttgcg
    11341 ttgcggtgta ggacgttgca gaatcggtct cgaccacggg agcccggtgt gagacgcgcc
    11401 cttgccaggc gaggcggcgg gatcgtgctg tcggtgttgt gtgccgcccc ggttctcgcc
    11461 ggcggtctcg acgacgtcgc ccggctcttc gagcgcaacc ggcagcagct acgcgattac
    11521 gtctggacct cgacgaccga gatcgccctc gagggcgaac ggccgacggt cgagcggtac
    11581 gaagtgcgcg tcgacgagaa cggggtacag cggcgaacca cggtcccgtc ggcggccggc
    11641 cgcggacgga aggcgggcaa gcgccagcgg caagccgaag agcttcacga cagcttgcgg
    11701 tcgttggtcg agtcctacgt ccatccggcc cccgagacga cccggactct gttcgctgac
    11761 ccgttcgtct ggcgcggtga agggcggatc gagggcgtca cccggctcca ggcgagaaac
    11821 gtcgtgcgcg agggggacca ggtcagcctg tggctcgaga ccgcgaccga gcggccgacg
    11881 aaactggaga tcctcacctc ctcgggcgga gagcccgtgc gggtgacgac cgagttcgcc
    11941 cggatcgacc aagggccgtt ctacccggcc cgcatcgtga tggagaccga gatcaaggag
    12001 aagaggctgg tcgtcaccac cgcgaacgcc gattaccgtc gcgcggaacg ggcgagcccc
    12061 tagcgttcgc acgttcgcga acctatagtc tcgatatgaa aagcccggaa ttcctatatt
    12121 cgggagtgga gttggtgcgg gcgcgtcgcg ggccggccgc ggcggttttc gccctcctcg
    12181 ggctggtcgt cgccggcccg cccgcgtccg gcgagccggc cgagcctccc gccggcgtgg
    12241 tccacgtcgt cgagcgaggg cagacgctgt ggcgcatcgc gcgcacctac gaagtcgaga
    12301 tcggcgagtt ggcccgcatc aacggcatcg ccgatcccac gcggatccac accggccagc
    12361 gcgtgttcgt tccgggcgtc gattcgttgc gcgtcgtcgt accgccgtcc gcgagccccg
    12421 tcctcgccgc ttccgcttgg gcgtggccgg tcgaggaggg tgtgctgctg tcctatttcg
    12481 gggcgccgcg gcggtcccgc gatcatcagg gtgtcgacat ccgcgcccgg cacggtcagc
    12541 cggtcgtggc cgcccgggcc ggccgcgtgg tctacagcgg cgccgggatg cgcggctacg
    12601 gcaagacggt gatcatcgat cacggcgacg gcatctcgac gctgtacgcc cacaactcgg
    12661 ccctggccgt ccgtgtcggt caacgggtgg cgcagggcga gcggatcgcc cgcgtcgggc
    12721 gctcgggcaa tgcctcctcc gatcactgtc acttcgagat tcgccaccag gatcggccgg
    12781 tcgaccccct gccctatctg gcccggtcgg aggcgcgtcg atgaacgtcg aggtccactg
    12841 caccgactgc aggacggcat acgtgctgga gggcggattc gccgggacga cgcttccgtg
    12901 tccggcctgc ggagcccccg acgggctcac cgtgccggcc gacgcgcccg aaccgccgtt
    12961 tcgcatcgca cggccggttg ccgcggccgt caccgcggcg cactcgagcg caccctccgt
    13021 cgtggccgag acgcgtcccg tcccaactcc gccggccgcg tccaccgggc cgtcgccggc
    13081 caccgttcgc gcgaagccga gcgagcgcgc ggcccaacgc tccaacgagg cggatgaggt
    13141 cgtctgtccc cgctgcaagt tgcacttcgt cccgcgcaag gggcagctcg agaccgggaa
    13201 ctcgggacgc cggtcggtgc tggtcgtcga agcgatggac tacttccgtg agatcgccgc
    13261 ggaagcgctg gcggaccact tcgcggtcaa gcaggccgcc gacgtcggcg aggcgcgtgc
    13321 gctgctcgac gccgaggagt tcgacctgtt ggtgctcgac ccggaaagcg gcgggatcga
    13381 gctgctgcgc ggcctgggtc gcaaaccgtg tccgattctg gtctataccg accgagacga
    13441 gtcggagatc tacggcgaca gctgggacga gctgcaacgc tacggcgcgg acgacatcgt
    13501 gatgaaggcg atgaacgcgg gggagacgct ggcgagaaag gcgagcgtgc tgctcggcgt
    13561 gcggatcgac gaggacgagt gatcccgagc ggcagggctc acacgccgcg agcggcgacc
    13621 gcgatcaaca ccagcgccat cgccgccaga atcaacagcg aaccgtcgat caagcgcgcc
    13681 cgcaggttgt cggcgattct ccggcccatc gcgcactcct gaccgcccca cgcgacccca
    13741 cttacagcaa gtcccgtgcc ggtcgatctc ggcggctgcc cgccgcgtcg cggctctctt
    13801 tcgcggcgtg cgcagtcgcg cggtgtcacc cggcggcgtc gcagcgtcct ctcggtgaca
    13861 aaaaaacggc cggtggccta ccgtggccac cggccgttcg atcactcgat gatggaacag
    13921 ctactcgagc acgcggatga cgacgcgacg gttctgcgct cgtccctcgg tcgaatcgtt
    13981 gttggccacc ggcttttcct ctcccagcga gatcacgttg atcgcgtgga gcgggacgcc
    14041 attcttcgcc atgtagtcgc gcacggtcat cgcgcgcttg tagcccaggt cgtagttgta
    14101 cgcgtcggtc ccggtcgcgt cggtgtggcc ctcgacctcg acgtagaccg ccttgtcgta
    14161 cgacatgatc ttgccgacga ggtcgtcgag cacccgggcg gcctcctgcg gcacgtcggc
    14221 ctgattgaac gagaacttga cctggtcgtt gttgagcgtc acctcccaga tcagtcgacc
    14281 gcgcaccgcc ttctccgcga cggtcatcgc gctctggcca acctccgtcg cgcgctcggt
    14341 cttggcgtcc aactcggcga tcttcgagtc cgtctcttcg ctcaggtcct tgatgcgtcg
    14401 ctcgttcgct tcgacaccgg actcgacggc ggccacccga gtgtcgttgg cctcgacgtt
    14461 cttccggaac atcttcttgc tgacgcagcc ggtcgaggcg acggaagcca cggccacgag
    14521 catcgccagc atcagcatcg ttcgtcttcg cattgactgc tctcccttgt gctcaaacca
    14581 gtcgtgagtc gccggactcg ctttcgacag agcctttact ttagaccgcg ggggcggccg
    14641 agcgcaatgt gggcgttgcg gcggcgcctc agggctccgc gtagtacccc cgccgcgcac
    14701 ggacgaccag ccggtcgttg gccgtttcga cctggatccg acggtaggag ccgtcccaca
    14761 gggtccgctc cgggcggtag ctgatcacgt agcggaaccg cagctcgcgc tggacggcga
    14821 gcgtggcctc cttcagatcg tcggggtcgc ggacgacgaa cagccgtcca ccggtctcgt
    14881 ccgcgaacaa cttcacgatg cggtgctcct ccggcgtgct gcccgacggg aggagacgtg
    14941 aggcgaaagt cgagaagccg atggcgtaga tcggcacgtt gaccgaccgg gccagcgtca
    15001 acgcgttcca cagattctgc ttgctcgccg tgtcgatccc gtcggtcaac agcacgatcg
    15061 ccttgctccc cgcctcctcg gcatcaagca ggcccggcgc ggcggccagc gcgtcgagca
    15121 gggcggtctg cccgtaaccg tgctgcaccg cgaggcgttc gaggaagcgc ctgcggtcac
    15181 gggtcagctc ggtcacccag tcgatctgtt cgtcggcgaa gcaaacgagg ccgagacggt
    15241 cgtccggccg cagcccgtca gcgaagacgc ggatcgcctg cttggc
//
LOCUS       DASPGN010000239         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1563302, whole genome shotgun sequence.
ACCESSION   DASPGN010000239 DASPGN010000000
VERSION     DASPGN010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563302"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VD788_06990"
     CDS             <1..868
                     /locus_tag="VD788_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HXV76054.1"
                     /translation="ASSLAKRFYGDYELAGLLLEYNGRRGSTVIHVDEQLAIPICPEH
                     RVRAGDTWSVLAARHLGRPDGWAAVALLNEMLPEKPLAIGSTIVFPVVMPYELRRGES
                     LAALARRFYGDSDRAEVLQRWNGIDDPRRLAVGASVEIPLVAFRATASGSRPASAAPA
                     SSAGAPAPDVATGVEPPAPEPPAPEPPPGLTFGEPLDDIAAALDEGRYDDARERIDAL
                     LDRVQAKGTLEAQARMWELVGRMRVAFDDYERACEAYRALAALASTPDLDADRVSPKI
                     RSVYRDCVAASR"
     gene            1017..1280
                     /locus_tag="VD788_06995"
     CDS             1017..1280
                     /locus_tag="VD788_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76055.1"
                     /translation="MNARNHWRQARFNNAEERSEFIEHARSIDLDGIEVEPVDNDDVR
                     VRFRAPAKFEIGLAGMVHAHGGKVLPSADADQAVPRPRAARSA"
     gene            complement(1221..>2044)
                     /locus_tag="VD788_07000"
     CDS             complement(1221..>2044)
                     /locus_tag="VD788_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76056.1"
                     /translation="FGAGSALADVGGDFEENTPYVLSIIDDPDPTGRSRSSRPRLLHS
                     TPAIELDHRRGDERPSLLAFPADDRPIVAWARKGRDGFDVVISSRVGGRWSEPVVLAG
                     SPADELDPRLAVDPRDGTVHVVFWVRDATPAVIHRQAPADLAQWSEPRFVSPPGEAAM
                     RPSPSIDDGRLRIVYEAHAAAEGSTPHLIVLATDGPGGFTTEVVGLSLHDGANLATID
                     GTGWRLRVRWDESLFEGIEAERLDDGRWLERRLDLPSGAPRRSRPWDSLVGVGRR"
BASE COUNT          305 a          746 c          705 g          288 t
ORIGIN      
        1 ggcctcgtcg ctggccaaac ggttctacgg cgactacgag ctggccggtt tgctgctcga
       61 gtacaacgga cggcgcggct cgaccgtgat ccacgtcgac gagcagctcg cgatcccgat
      121 ctgtcccgag caccgcgtgc gtgcgggcga cacctggtcc gtgctcgccg cgcgccatct
      181 cggccggccc gacggctggg ccgcagtggc gttgctcaac gaaatgctgc cggaaaagcc
      241 gctcgcgatc ggcagcacga tcgtcttccc cgtcgtgatg ccctacgagc tgcgccgcgg
      301 ggagtcgctg gccgcgctcg cccggcggtt ctacggcgac agcgatcgcg ccgaggtcct
      361 gcagcgctgg aacgggatcg acgatccgcg ccggctggcg gtcggcgcgt ccgtcgagat
      421 cccgctcgtc gcgttccgcg cgacggcttc cgggtctcga ccggcgtccg cggcgcccgc
      481 gtcgtccgcc ggcgcgccgg cacccgacgt ggcgaccggc gtcgagccgc cagcgcccga
      541 gccgccggcg cccgagccgc cgccgggcct caccttcggc gagccgctcg acgacatcgc
      601 ggcggcgctc gacgaaggac gttacgatga cgcacgcgag aggatcgacg ccctgctcga
      661 ccgcgttcag gccaagggaa cgctcgaggc gcaggcccgc atgtgggagt tggtcgggcg
      721 catgcgcgtc gccttcgacg actacgagcg cgcgtgcgag gcctaccgcg ctctcgcggc
      781 gttggcgtcg acacccgacc tcgacgcgga tcgcgtgtcg cccaagatcc gctcggtcta
      841 tcgcgattgc gtcgcggcct cacgctgacg taacgcgtct taccgtttcg caaccacgcc
      901 ttcgcgcaac gatcgtcgga cgacgaaatt gaatcggccg gcgccttgac cgcgcgtcga
      961 tacggtcccg atatttacgg ctgacgcgaa ccactccacc gatcgggaga gagcccatga
     1021 acgctcggaa ccactggcgc caggccaggt tcaacaacgc cgaagagcgc tcggagttca
     1081 tcgagcacgc ccgctcgatc gacctcgacg ggatcgaggt cgagccggtc gacaacgacg
     1141 acgtgcgcgt gcgtttccgc gcacctgcga agttcgagat cggcctggcc ggcatggtcc
     1201 acgcccacgg cggcaaggtg ctaccgtcgg ccgacgccga ccaggctgtc ccacggccgc
     1261 gagcggcgag gagcgcctga cggtaggtcg agccgtcgtt cgagccaccg cccgtcgtcg
     1321 agccgctcgg cttcgatccc ctcgaagagg ctctcgtccc acctcacccg caaccgccaa
     1381 ccggtgccgt cgatcgtcgc cagattggca ccgtcgtgga gactgagacc gacgacctcg
     1441 gtcgtgaagc cccccgggcc gtccgtcgcc aggacgatca ggtgcggcgt cgagccttcc
     1501 gcggcggcgt gcgcctcgta cacgatgcgc agtcgcccgt cgtcgatcga cggcgatggc
     1561 ctcattgcgg cctcacccgg gggtgaaacg aagcgcggtt cgctccactg cgcgaggtcg
     1621 gcgggcgctt gccggtgaat gacggccgga gtcgcatcgc ggacccagaa cacgacgtgc
     1681 accgtcccgt cccgcggatc gaccgccagc cgcgggtcga gctcgtccgc cggcgatccg
     1741 gcgagcacca ccggctcgct ccagcgccct cccacgcgag agctgatcac gacgtcgaag
     1801 ccatcgcgcc ctttccgcgc ccacgcgacg atcggccggt cgtcggcggg aaacgcgagg
     1861 agcgaaggcc gctcgtcgcc gcggcggtgg tcgagctcga tcgccggggt ggagtggagc
     1921 aaccgcggcc gcgaggagcg cgagcgcccg gtcgggtcgg ggtcgtcgat gatgctcagg
     1981 acgtagggcg tgttctcctc gaaatcaccg ccgacatcgg cgagggccga accggcgccg
     2041 aacg
//
LOCUS       DASPGN010000240         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_627014, whole genome shotgun sequence.
ACCESSION   DASPGN010000240 DASPGN010000000
VERSION     DASPGN010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627014"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /gene="tuf"
                     /locus_tag="VD788_07005"
     CDS             <1..141
                     /gene="tuf"
                     /locus_tag="VD788_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HXV76057.1"
                     /translation="EMVMPGDNVTIEVNLITPIAMDKGLRFAIREGGRTVGAGTVTEI
                     VE"
     gene            242..568
                     /gene="rpsJ"
                     /locus_tag="VD788_07010"
     CDS             242..568
                     /gene="rpsJ"
                     /locus_tag="VD788_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HXV76058.1"
                     /translation="MVNEKIRIRLKAYDHRILDQSTSEIVDTARRTGARVAGPIPLPT
                     VRNRWTVLRSPHVDKKSREQFEIRTHKRLLDILQPTPETVDALMKLDLPAGVDVEIKA
                     FGQGQA"
     gene            600..1271
                     /gene="rplC"
                     /locus_tag="VD788_07015"
     CDS             600..1271
                     /gene="rplC"
                     /locus_tag="VD788_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HXV76059.1"
                     /translation="MEGLIGRKLGMTQLFDEDGTVTPVTVIAAGPCVVTQLRTSARDG
                     YDAAQLGLVDAKAAKLANKPMRGHHEQAGVPPTRLRREFPVVAGGGEARELKAGDPVT
                     VEIFAGVSHVDVIGVSKGKGFQGVIRRHGFAGGRATHGSMFHRAPGSIGQSAWPSRVL
                     KGMRAAGRMGGDRVTLKNVKVVRVDAEKNLLLVRGSIPGHPGSTVVVSRARRESLGAA
                     GNRGA"
     gene            1274..1903
                     /gene="rplD"
                     /locus_tag="VD788_07020"
     CDS             1274..1903
                     /gene="rplD"
                     /locus_tag="VD788_07020"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXV76060.1"
                     /translation="MPEIAVRNWKNEPVRSLDLDASVFDYPLKKHLIYEAVLAYRAAA
                     RAGTHKVKNRVEVSGGTRKLWKQKHTGRARMGDNRSPLWRHGGTVHGPQPRDYSWKFP
                     KRMRRNALRSALAQKLREGKLVCIEAFDLPSHKTQEFDRAVAGGLGIHGKTLMVPAET
                     EEKLELATRNNPRFTAVRALGVSVVDVLGHDTLLFSEEALRRLSEVLAK"
     gene            1900..2202
                     /gene="rplW"
                     /locus_tag="VD788_07025"
     CDS             1900..2202
                     /gene="rplW"
                     /locus_tag="VD788_07025"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HXV76061.1"
                     /translation="MINPFRTIRQPLITEKSTIQREETGTYLFKVHIRANKQEIARAV
                     EQLFAKEKVKVADVRTAVVRGKGKRMGRFEGRRPSWKKAWVKLTPDSGEIEFFEAS"
     gene            2218..3045
                     /gene="rplB"
                     /locus_tag="VD788_07030"
     CDS             2218..3045
                     /gene="rplB"
                     /locus_tag="VD788_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXV76062.1"
                     /translation="MAIKNYKPTSPGRRFQTRIVRATEIASEPHKPLVEKLHKRGGRD
                     NSGRIAVRHQGGGHKRRYRVIDFRRDKVGIPAKVERIEYDPNRSADIALVCYHDGERR
                     YILAPHGLANGQIIVSSPEADIQPGNALPLRAIPLGTTVHNVELRPGKGGQLARSAGT
                     GAQLVAKEGRFALLRMPSGEMRRVLATCYATVGQVGNTDHENQSIGKAGRNRWQGRRP
                     TVRGVVMNPVDHPHGGGEGRTSGGRHPVTPWGVPTKGKKTRNNPRTDAMIVRRRGKK"
     gene            3058..>3222
                     /locus_tag="VD788_07035"
     CDS             3058..>3222
                     /locus_tag="VD788_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S19 family protein"
                     /protein_id="HXV76063.1"
                     /translation="MARSISKGPFVDEHLRRKVDELNRRNDKKVVKTWSRRSTVTPEM
                     VAHTIAVHNGK"
BASE COUNT          614 a         1031 c         1150 g          427 t
ORIGIN      
        1 gagatggtga tgcccgggga caacgtgacg atcgaggtga acctgatcac gccgatcgcg
       61 atggacaagg gcctgcggtt cgcgatccgc gagggcgggc ggacggtcgg cgccgggacc
      121 gtcaccgaga tcgtcgagta gagagacctc ctgcctcgct cgattcggcc gcccgtcgga
      181 cgggaacgcg gatcgagcag cgggatcgag cctccagccg tggttggagg gcaggacgaa
      241 gatggtgaac gaaaagatcc gcattcgcct caaggcgtac gaccaccgga tcctcgacca
      301 gtcgacgagc gagatcgtcg acaccgcccg gcgcacgggc gcgcgcgtcg ccggcccgat
      361 cccgttgccg acggtccgca accgctggac ggtcctgcgc tcgccccacg tcgacaagaa
      421 atcgcgcgag caattcgaga tccggacgca caagcggctg ctcgacatcc tgcagccgac
      481 gccggagacg gtcgatgcct tgatgaaact ggatcttccg gccggagtcg acgtcgagat
      541 caaggcgttc ggccagggtc aggcctgaga cccgccggcc gcacgagggt ggacagatga
      601 tcgaaggact gatcggacga aagctcggga tgacccaact gttcgacgag gacggtacgg
      661 tcacgccggt gacggtgatc gcggccggcc cgtgcgtcgt cacgcagctc aggaccagcg
      721 cgcgcgacgg ctacgacgcg gcccagctcg ggctggtcga cgccaaagcc gccaagttgg
      781 ccaacaagcc gatgcgcggt catcacgagc aggccggcgt accgccgacg cgcctgcgac
      841 gcgagtttcc cgtcgtcgcc ggcggtggcg aggcgcgcga gctcaaggcc ggagacccgg
      901 tcaccgtcga gatcttcgcc ggcgtgagtc acgtcgacgt gatcggcgtg tcgaagggca
      961 aggggtttca gggtgtcatc cgccgccacg gcttcgccgg tggccgggcg acgcacgggt
     1021 cgatgttcca ccgcgcgccg ggctcgatcg gtcagtcggc ctggccgtcc cgggtgttga
     1081 agggaatgcg cgccgccggc cggatgggtg gcgaccgcgt gacgctgaag aacgtcaagg
     1141 tcgtccgcgt cgacgcggaa aagaacctgc tgctggtacg cgggtcgata ccggggcatc
     1201 cgggatcgac cgtggtggtg tcacgcgccc ggcgcgagtc gctcggcgcg gccggaaacc
     1261 gaggggcatg acgatgccgg aaattgccgt gcgcaactgg aagaacgagc cggttcgctc
     1321 gctcgatctc gacgcctcgg tcttcgacta cccgttgaag aagcacctga tctacgaggc
     1381 ggtgctggcc taccgcgccg cggcccgagc gggcacgcac aaggtcaaga accgcgtcga
     1441 ggtctccggc ggaacccgca agctgtggaa gcagaagcac accgggcgcg cgcggatggg
     1501 cgacaatcgc tcgccgctgt ggcggcacgg cggcacggtc cacggcccgc aaccgcgcga
     1561 ctactcgtgg aagttcccca agcgcatgcg gcgcaacgcg ttgcgctcgg cgctggcgca
     1621 gaagctgcgc gaagggaagt tggtctgcat cgaggcgttc gatctgccgt cacacaagac
     1681 gcaagagttc gaccgcgccg tggccggcgg gctcggcatc cacggcaaga cgttgatggt
     1741 gccggccgag accgaggaaa agctcgaact ggcaacgcgc aacaacccgc ggttcacggc
     1801 ggtgcgggct ctcggggtca gcgtcgtcga cgtgctggga cacgacacgt tgctgttctc
     1861 cgaggaggcc ctgcgccggc tgagcgaggt gcttgcgaaa tgatcaatcc gttccgcacg
     1921 atccgccaac cgttgatcac cgagaaatcg acgatccagc gggaggagac cggcacctac
     1981 ctgttcaagg tgcacatccg ggcgaacaag caggagatcg ctcgcgccgt cgagcagctg
     2041 ttcgccaagg aaaaagtcaa ggtggccgac gtccgcacgg cggtcgtgcg cggcaagggc
     2101 aagcggatgg ggcgctttga gggacgacga ccgagctgga agaaggcctg ggtcaagctg
     2161 acgccggatt cgggcgagat cgaattcttc gaagcgagct agcgagggac agccacgatg
     2221 gcgatcaaga actacaagcc gaccagcccg ggaagacgat tccagacgcg gatcgtccgc
     2281 gcgaccgaga tcgcgagcga gccgcacaag ccgctggtcg agaagctgca caagcgaggc
     2341 gggcgcgata actccggccg catcgccgtg cgtcatcagg gcggcggaca caagcggcgc
     2401 tatcgcgtga tcgacttccg ccgggacaag gtcggcatcc cggccaaggt cgagcggatc
     2461 gaatacgatc cgaatcgcag cgccgacatc gcgctggtct gctaccacga cggcgagcgg
     2521 cgctacatcc tcgccccgca cggcctggcc aacggtcaga tcatcgtctc ctcgccggag
     2581 gccgacatcc agccgggcaa cgccctgccg ctccgcgcca tcccgctcgg gacgacggtg
     2641 cacaacgtcg agctgagacc gggcaagggc gggcagttgg cccgctcggc cggaaccggc
     2701 gcgcagctcg tcgccaagga aggtcgtttc gccttgctgc ggatgccgtc gggggagatg
     2761 cgtcgcgtgc tggcgacctg ttacgcgacg gtcggccagg tcggcaacac cgaccacgag
     2821 aaccagtcga tcggtaaggc cgggcgaaac cgctggcagg gccgccggcc gacggtgcgc
     2881 ggcgtcgtga tgaacccggt cgaccatccg catggcgggg gagaagggcg cacctcgggc
     2941 ggccggcatc cggtcacgcc gtggggcgtg ccgaccaagg gcaagaagac gcggaacaac
     3001 ccgcggacgg acgcgatgat cgtccgtcgt cgcgggaaga agtaacggag gccgtagatg
     3061 gcacgctcga tttccaaggg tccgttcgtc gacgagcacc tgcgccgcaa ggtcgacgag
     3121 ctgaaccgcc ggaacgacaa gaaagtggtc aagacctggt cgcgccgctc gacggtcacg
     3181 ccggagatgg tcgcccacac gatcgcggtg cacaacggca ag
//
LOCUS       DASPGN010000241         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_978112, whole genome shotgun sequence.
ACCESSION   DASPGN010000241 DASPGN010000000
VERSION     DASPGN010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978112"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VD788_07040"
     CDS             complement(<1..465)
                     /locus_tag="VD788_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HXV76064.1"
                     /translation="MGFPVPELALRRLPLLLVALAPLAHADTGHDLRVAARSGDLAEV
                     RRLVEAGADVDAADSWGTTPLLLAAKQQQVDVVRYLLAKAADPDARERFFGSTVLEQA
                     LDDGAPDYEVARMLLEAGADDRAAALDTAFGEGRLELARAATASGPVLESEAK"
     gene            617..693
                     /locus_tag="VD788_07045"
     tRNA            617..693
                     /locus_tag="VD788_07045"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:651..653,aa:Pro,seq:tgg)
     gene            911..1519
                     /locus_tag="VD788_07050"
     CDS             911..1519
                     /locus_tag="VD788_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HXV76065.1"
                     /translation="MKRFPSTVHVIGAGGHAKVVVATIRAAGGEVGRILDDDPALHGR
                     RLLGAAIGGPVTMDLLRGAPAVIAVGDNATRRELARRLDAEWIAVCHPSAVVHASVTI
                     GPGTVVFAGVVIQPDTTIGAHAIVNTGATIDHDCRLGDFVHVGPGVSLCGGVAVGDGA
                     LLGVGARVCPSLRIGAGAVVGAGAACVADVADGARVVGVPAR"
     gene            1547..2041
                     /locus_tag="VD788_07055"
     CDS             1547..2041
                     /locus_tag="VD788_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76066.1"
                     /translation="MSPPDRQRLISGMTLILLGAGLFWLHRSEGIGEAAVFFIVGGSF
                     LAAYLYRRNYGLLVPAGILLGLGAGVVGRDSLFAFGQARLLGLGFGFVAIFVIALAYE
                     RKTAWWPLIPGAALILLGIPDTEKIFDFLHRNWPLLLVVAGVLVLFGAFGKPPSRGGE
                     RAGR"
     gene            2187..>3236
                     /locus_tag="VD788_07060"
     CDS             2187..>3236
                     /locus_tag="VD788_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HXV76067.1"
                     /translation="MTGLYLVCATLLAVATSIAAAETDCAPAGASPLPTGVTPRVEIV
                     FALDATGSMSGLIEGAKDKVWSIVNAVARARPAPEVVVGMLVYRDRGDEYVTRRTPLD
                     ADLDRGYAWLIDQQAAGGGDGPESVRQALHEAVAQFNWSTTPGGLRLIFLVGDAPPHD
                     DYHDDVTLSESIALATERGIRINTIQCGGWAETTGVWKAIADASGGDYFQVEQSGGMV
                     ARATPFDAELARHARLFDETIVPYGTPDEAPPSVDDEASAEALAERAAFKLSPAGESG
                     LVARGDLVRQVLDGAIDVSKVDRDLLPEPLGSLSDDELRDYLAMLAERRRGLREELEW
                     LTAQRNDALAARPSGD"
BASE COUNT          420 a         1132 c         1185 g          499 t
ORIGIN      
        1 cttcgcttcg gactcgagca ccggtccgct cgccgtcgcc gcgcgtgcca gctcgagtcg
       61 accttcgccg aacgcggtgt cgagcgccgc cgcacgatcg tcggcacccg cctcgagcag
      121 catccgcgcc acttcgtagt ccggcgcgcc gtcgtcgagc gcctgctcga gcacggtcga
      181 accgaagaag cgctccctgg cgtcggggtc ggccgccttg gccagcaggt aacgcacgac
      241 gtcgacctgc tgctgcttgg cggcgagcag caacggggtc gtgccccagc tgtcggcggc
      301 gtcgacgtcg gcgccggcct cgaccagccg ccggacctcg gccaggtcgc ccgagcgtgc
      361 ggcgacgcgc aggtcgtgcc ccgtgtccgc gtgcgccagc ggcgcgagcg cgacgaggag
      421 cagcggcagc ctgcggagcg cgagctcggg cacgggaaac cccaccggcg atcgcccgac
      481 ccgtcccggg tcggatcgcc ccgacgattc tacgccacgc cgccacccgg ccgcgggctc
      541 gccgcgccgc gggatcctcg gccgaggcga tcgtcggttg ccccctccgg caccaggcga
      601 catactgtcg gcggatcggg gcgtagcgca gtctggtagc gcatctgctt tgggagcaga
      661 gggtcgcgtg ttcgaatcac gccgccccga ccacctacgg ccgccacggt caagaaaggt
      721 gttccacccc gacccgccgg ctgtcccact cgtgggaggg atcagtcgac ggggaggcac
      781 ggtcgatacg gtcgaaccgg ttccggtcac accttaccgg ctcggtccga tccctctcaa
      841 ttccccattc cgtccgttcc cgatcgcgat cgggtgtagg cttgaggcga cccgccagcg
      901 gaggtcgccc gtgaagcgtt tcccgagcac ggtccacgtc atcggagccg gcgggcacgc
      961 caaggtggtg gtggcgacga tccgggccgc gggcggggag gtcggtcgga tcctggacga
     1021 cgacccggcg ctccacggca gacggttgct cggcgcggcg atcggcggcc cggtcaccat
     1081 ggatctcctc cgcggcgcgc cggcggtcat cgcggtcggc gacaacgcga cgcgccgcga
     1141 gctggcccga cgtctcgacg cggagtggat cgccgtctgc catccgagcg ccgtcgtcca
     1201 tgcgagcgtg acgatcgggc cgggcacggt cgtcttcgcc ggggtcgtga tccagcccga
     1261 cacgacgatc ggcgcccacg cgatcgtcaa taccggcgcg acgatcgacc acgactgtcg
     1321 cttgggcgac ttcgtccacg tcggaccggg ggtgtcgctg tgcggcggcg tggccgtcgg
     1381 cgacggcgcg ttgctcggcg tcggtgcccg ggtctgcccg tcgctgcgca tcggtgcggg
     1441 cgcggtcgtc ggtgccggcg ccgcctgcgt tgccgacgtc gccgacggcg cgcgcgtcgt
     1501 cggcgtgccg gcccgctgat cgtgcgaagc gtgggacaat gaagggatga gcccacccga
     1561 tcggcaacgg ctgatctccg gcatgacgtt gatcctgctc ggcgccggtc tgttctggct
     1621 tcaccggagc gaggggatcg gcgaggcggc ggtgttcttc atcgtcggcg ggtcgtttct
     1681 cgccgcctat ctctaccggc gcaactacgg cttgctggtg ccggccggca tcctgctcgg
     1741 cctcggcgcc ggtgtggtcg ggcgcgacag tttgttcgcc ttcggtcagg cccggctgtt
     1801 ggggctcggt ttcggcttcg tcgcgatctt cgtcatcgcg ctggcctacg agcgcaagac
     1861 cgcctggtgg ccgttgattc ccggtgcggc gttgatcctg ctcggcatcc cggataccga
     1921 gaagatcttc gacttcctcc accgcaactg gccgctgctg ctggtggtcg ccggcgtgct
     1981 cgtgctgttc ggcgcgttcg gcaagccgcc gtcgcgcgga ggcgaacggg ccggacgctg
     2041 agctgcgtgt tcgggcaacc acgccggtgc ggccgaacgg caacggccgg tgtcgcgtgg
     2101 gcgcctggtg cggcccgaat ctcgttggca gaccctttgc actcgaggac gggcagtgac
     2161 tcggggggcg tcgtcggagt gccacgatga ccggtcttta cctcgtctgc gcgaccttgc
     2221 tcgcggtggc gacttcgatc gcggcggccg agaccgactg cgctccggcc ggcgcgagcc
     2281 cgctgccgac cggcgtcacg ccgcgggtcg agatcgtctt cgcgctcgat gccaccggca
     2341 gcatgagcgg cctgatcgaa ggcgccaagg acaaggtctg gtcgatcgtc aacgcggtcg
     2401 cgcgcgcccg ccccgcgccc gaggtcgtgg tcgggatgtt ggtctaccgc gatcgcggcg
     2461 acgagtacgt cacccgccgg acgccgctcg acgccgacct cgatcgtggc tacgcctggt
     2521 tgatcgatca gcaggccgcg ggcggcggcg acgggccgga gagcgtgcgc caagcgctgc
     2581 acgaggcggt ggcgcagttc aattggtcga cgacgcccgg cggactgcgg ctgatcttcc
     2641 tcgtcggcga cgcgccaccg cacgacgact accacgacga cgtgactctg tccgagtcga
     2701 tcgcgctcgc gaccgagcgc gggatccgca tcaacaccat ccagtgcggc ggttgggccg
     2761 agacgaccgg ggtctggaag gcgatcgccg acgcctcggg cggtgactac ttccaggtcg
     2821 agcagagcgg cgggatggtg gcgcgagcga cgccgttcga cgcggagctg gcgcgacacg
     2881 cgcggttgtt cgacgagacg atcgtcccgt acggaacacc ggacgaagcg ccgccgtcgg
     2941 tcgacgacga ggcgtccgcc gaggcactgg ccgagcgcgc tgcgttcaag ctgtcgcccg
     3001 cgggggagtc gggtctcgtc gcacgcggcg atctggtgcg acaggtgctc gacggcgcga
     3061 tcgacgtgtc gaaggtcgac cgcgacctgc tgcccgaacc gctcggctcg ctgagcgacg
     3121 acgagctgcg ggactatctg gcgatgctgg ccgagcggag gcgcgggttg cgcgaggagc
     3181 tcgagtggct caccgcgcaa cggaacgacg ccctcgccgc gcgaccgtcc ggcgac
//
LOCUS       DASPGN010000242         3920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_627607, whole genome shotgun sequence.
ACCESSION   DASPGN010000242 DASPGN010000000
VERSION     DASPGN010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627607"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VD788_07065"
     CDS             <1..1129
                     /locus_tag="VD788_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV76068.1"
                     /translation="GVILALATGLAATACSSDVKIGAVISESGAVEAYGKRVRKGMEL
                     AAEEINAGGGLASGGQLLLVFRDDATNPERGKQVTQELIDQEGIDLIVGAVSSRVTLA
                     IAPICEKERVLLISPSSSSPDISQAGEYIYRNYPSDIREGTSMAKFAKDLALEQVAIV
                     AMDDEFGRGLREVFTQQYEGRSRQVIGSFEFDGGAAEFDAIVTQVTALAPDGIYLVGY
                     ENEVSQLLTELRAAEVAAVIMTSSAVTPNLVQLAGAATDNVVFPQTTFDLDSDAPSVA
                     AFVTAYRQKYDEDPDPYAAHGYDAVKVLAEAINSVEGSHPDDVRIGLNGIQDYDGAAG
                     KTTFDKNGDVVRHPRIMIFRNGAAMPYDEFVEEGGSILPQG"
     gene            1143..1919
                     /locus_tag="VD788_07070"
     CDS             1143..1919
                     /locus_tag="VD788_07070"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HXV76069.1"
                     /translation="MEAGGRPHLSVVVPAFNEESRLGGSLTAILSYLDRHKLHAEVLV
                     VDDGSADGTQRVADELLSFPRGRVLRNDENRGKGFSVRRAVLESRGRWVLMTDADLST
                     PIEEHQRLAAAARDRDLDIVIGSRGLPDSQVEVRQSVMRQTMGRSFNLLVRSITGLPF
                     RDTQCGFKLLDRERVLKIFERMFVDGFAFDVELLYVAHRFGLRIAELPVVWRNSPGST
                     VAVTSAPPAMLRDVLRVLWRFRRGGYAPRSDAAPSSPAHG"
     gene            1912..>3920
                     /locus_tag="VD788_07075"
     CDS             1912..>3920
                     /locus_tag="VD788_07075"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXV76070.1"
                     /translation="MASDLALWSPLTRAPFFEQLERRSAAGEPLCLTGLVEGSRALVL
                     SLWVARGGGPLILVVPDDSALAAYGRDLAAFATVLGRDPSRIVTMPALDADPYDNIAP
                     HPEVVRERVLALDRLGRGALDVLLLPARTLLNPLPDPERWRSWTRVISTGDDLPPERF
                     VLSMMELGYRRVDIVGAPGEISRRGGIVDVFPPTAEQPVRIELFGDAVDSLRAFDTDN
                     QRSTGQLDRVVVVPASENPPTEAAIERLASRLEGAIRRTDAARSARAMREQLARLQGE
                     GIWPGFESLAGLACDRPGSLLDYAIGHCWIVDEAARTEEELLRAAHESRMTYEQSDNR
                     WLPPPDELFRDPVEVRERLGSAALFLQELAGDEPPQVRRVHAVPSRSARHYAGRVPEL
                     VADLGRAAAEGVRTVCLMRAPGSAGRLREILAEYDLVAGAVEQPIEPAREAEAGGGLF
                     VGVAGLRAGFELPQAKLVVLAERDVFAEEPKSADRKPKGRTAFLSDFRDLKPGSFVVH
                     VDHGIARYGGLGRPKGGSLNRDFMVLEFDRGDRLFVPVDRLDLVQKYNGVAGAKPRPD
                     RLGGPGWSRLRTRVRESVESMARELLELYARRQAARGIAFSPDTPWQQELEGAFPFEL
                     TPDQRRALAEIKADMEASRTMDRLLVGDVGFGKTEVALRAAFAAA"
BASE COUNT          570 a         1345 c         1392 g          613 t
ORIGIN      
        1 gggcgtgatc ctggcgttgg cgacgggcct cgccgcgacc gcttgctcga gtgacgtgaa
       61 gatcggcgcc gtcatctcgg aaagcggggc ggtcgaggcg tacggcaagc gtgtgcgcaa
      121 ggggatggaa ctcgccgccg aggagatcaa cgccgggggc ggtctggcca gcggggggca
      181 gctgttgctg gtcttccgcg atgatgcgac caatccggag cgcggcaaac aggttacaca
      241 ggagctgatc gaccaagaag gtatcgacct gatcgtcggt gccgtttcct cacgcgtgac
      301 gctggcgatc gcgccgatct gcgaaaagga gcgcgtgctg ttgatctcgc cgtcgtcctc
      361 gtcgcccgac atctcgcagg ccggcgagta catctatcgc aactacccgt cggacatccg
      421 cgagggcacg tcgatggcga agttcgccaa ggacctcgcg ctcgagcagg tggcgatcgt
      481 cgcgatggac gacgagttcg gccgcggtct gcgcgaggtc ttcacccagc agtacgaggg
      541 ccgctcgcgc caggtcatcg gcagcttcga gttcgacggc ggcgcggccg agttcgacgc
      601 catcgtgacg caggtcacag cgctcgcgcc ggacgggatc tacctcgtcg gctacgaaaa
      661 cgaggtcagc caactgctga ccgagctgcg ggcggccgag gtcgcggcgg tgatcatgac
      721 cagctccgcc gtgacgccga acctggtcca gctggccggc gccgcgacgg acaacgtcgt
      781 cttcccacag acgaccttcg acctcgactc ggacgctccg tcggtcgcgg cgttcgtgac
      841 ggcctatcgc cagaagtacg acgaggaccc ggacccctac gcggctcacg gctacgacgc
      901 ggtgaaggtc ctggccgagg cgatcaacag cgtcgagggg tcgcaccccg acgacgtcag
      961 gatcggtctc aacgggatcc aggactacga cggcgccgcg ggcaagacga ccttcgacaa
     1021 gaacggagac gtggtgcgtc acccgaggat catgatcttc cgcaacggtg ccgcgatgcc
     1081 ctacgacgag ttcgtcgagg aaggcggctc gattctcccg cagggctgag gccggcgcgg
     1141 tcgtggaagc cggcggccga ccgcacctgt ccgtggtcgt tccggccttc aacgaggaat
     1201 cccgtctggg cggctcgctg acggccatcc tgtcctacct cgatcggcac aagctacacg
     1261 ccgaagtgct ggtggtcgac gacgggtccg ccgacggtac gcagcgcgtg gccgacgagc
     1321 ttctttcctt cccgcgcggc cgcgtgctgc gcaacgacga gaaccgcggc aagggattct
     1381 cggttcgccg cgcggtgctc gaatcgcgcg gccgctgggt gttgatgacc gacgccgacc
     1441 tgtcgacgcc gatcgaggag caccagcgcc tggccgccgc cgcccgcgac cgcgatctcg
     1501 acatcgtgat cggctcgcgg ggtctccccg attcccaggt cgaggtccgg cagagcgtca
     1561 tgcggcagac gatggggcgt tcgttcaacc tgctcgtgcg ctcgatcacc ggacttccgt
     1621 ttcgcgacac ccagtgcggg ttcaagctgc tggaccgcga gcgagtgctc aagatcttcg
     1681 agaggatgtt cgtcgacggt ttcgccttcg acgtcgagct gctctacgtc gcccatcgat
     1741 tcgggctgcg gatcgccgag ctaccggtcg tctggcgcaa ctcgccgggg tcgaccgtgg
     1801 ccgtcacctc ggctcctccc gccatgctgc gtgacgtgct gcgcgtcctg tggaggtttc
     1861 gccgcggggg ctacgcaccc cgatccgacg ccgcgccctc gtcgccggcg catggctagc
     1921 gacctggcgc tctggtcgcc gctgacacgg gctccgttct tcgaacagct cgagcgacga
     1981 tcggcggccg gcgagccgct gtgcctgacc ggtctcgtcg aggggtcgcg ggcgctggtg
     2041 ctgagtttgt gggtggcgcg cggtggcggc ccgctgatcc tggtcgtgcc ggacgacagc
     2101 gcgctcgcgg cctacgggcg cgacctcgcc gccttcgcga ccgtgctcgg acgcgacccg
     2161 tcgcggatcg tcacgatgcc cgcgctcgat gccgacccgt acgacaacat cgcaccgcac
     2221 ccggaggtcg tgcgggagcg cgtgctggcg ctcgatcgtc tgggtcgcgg cgcgctcgac
     2281 gtcctgctgc tgccggcgcg cacgttgctc aacccgctgc ccgatcccga acgctggcga
     2341 tcgtggaccc gcgtgatctc gaccggcgac gacctgccgc ccgaacgttt cgtgttgtcg
     2401 atgatggagc tcggctaccg gcgcgtcgac atcgtcggtg caccggggga gatctcgcgc
     2461 cgcggtggaa tcgtcgacgt ctttccaccg accgccgaac aaccggtgcg gatcgagctg
     2521 ttcggtgacg cggtcgactc cctgcgtgcg ttcgacaccg acaaccagcg ctcgaccggc
     2581 cagctcgacc gggtggtcgt cgtgccggcc agcgagaatc cgccgaccga agccgcgatc
     2641 gagcgtctcg cctcacggct cgagggggcg atccgccgca ccgacgccgc ccggtcggcg
     2701 cgcgcgatgc gcgagcaact ggcacggctg caaggcgagg gcatctggcc cgggttcgag
     2761 tcgctcgcag gtctcgcctg cgaccgcccc gggtcgctgc tggactacgc aatcggccat
     2821 tgctggatcg tcgacgaagc ggcgcgcacg gaggaagagc tgctgcgggc cgcccacgag
     2881 tcgcgcatga cctacgagca gagcgacaac cgctggctgc cgccgccgga cgagctgttc
     2941 cgggatccgg tcgaggtgcg ggagcggctg ggctcggccg cgctgttcct gcaggagctg
     3001 gccggcgacg aaccgccgca ggtacgccgg gtgcacgccg tcccgtcgcg gagcgcacgg
     3061 cactacgcgg gccgcgtgcc cgagctcgtc gccgatctgg gccgggcggc ggcagagggg
     3121 gtacgaaccg tctgtctgat gcgcgcgccg ggaagcgccg ggagactgcg cgagatcctg
     3181 gccgagtacg acctcgtcgc cggcgcggtc gaacagccga tcgagccggc ccgggaggct
     3241 gaggccggcg ggggtctgtt cgtcggcgtc gccgggttgc gtgccgggtt cgagctcccg
     3301 caagccaagc tggtcgtgct ggccgaacgc gacgtcttcg ccgaggagcc caagtccgcc
     3361 gatcgcaaac cgaaggggcg caccgcgttc ctctccgact tccgcgacct gaagccggga
     3421 tcgttcgtcg tccacgtcga tcacggcatc gcgcgttacg gcggcctggg tcgcccgaag
     3481 ggcggcagtc tgaaccgcga cttcatggtg ctcgagttcg accgcggcga ccgtctgttc
     3541 gtacccgtcg accggctcga cctcgtgcag aagtacaacg gcgtggccgg ggccaaaccc
     3601 cgccccgacc ggctgggcgg ccccggttgg tcgcgcctgc ggacccgggt gcgcgagtcg
     3661 gtcgagtcga tggcgcgcga gctgctcgag ctgtacgcca gacgccaggc cgcccgcggt
     3721 atcgccttct cgccggacac gccgtggcag caggagctcg agggtgcctt tccgttcgag
     3781 ctgacgcccg accaacgacg cgcgctcgcc gagatcaagg ccgacatgga ggcgtcgcgg
     3841 acgatggacc gtctgctggt cggggacgtc ggcttcggca agaccgaggt ggcgttgcgc
     3901 gccgccttcg ccgccgcctt
//
LOCUS       DASPGN010000243        12615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_510804, whole genome shotgun sequence.
ACCESSION   DASPGN010000243 DASPGN010000000
VERSION     DASPGN010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12615
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510804"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VD788_07080"
     CDS             <1..585
                     /locus_tag="VD788_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76071.1"
                     /translation="AVSGTSALIHVMLSVLLGLAALAVGVTTAELMGQTLERAASVLL
                     VVFGSVYVWWAWRKGGHFHPGGRRLHGSHEREECDGREGDGNPEHLHYHADTALIADR
                     GAWWLAFVIGVNPCVLILPVIFASARGGPAAVAAVCAAYAVPTALLMVGLSAAGVAGG
                     EKLSLPLAARYAEVASGLVIAGLGVFLLVAHGSH"
     gene            745..1683
                     /locus_tag="VD788_07085"
     CDS             745..1683
                     /locus_tag="VD788_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV76072.1"
                     /translation="MIEIRDLWKNYGETQALRGVGFSIRRGEVVGLLGPNGAGKTTTM
                     KIVVGYLTPSRGTATVAGIDVGRDPLGVQRLIGYLPENAPLYSDMLAQEYLEFMADVR
                     QLAAVERRARIEQVVDECAIQSVLTRPIGHLSKGFRQRVGLASTLLHDPPILILDEPT
                     GGLDPNQIVEVRELIRRLGRTKTIILSTHILSEVEMSCGRAVIVIDGRVRADGSLDEL
                     TRAKAQLVTVKTGDPSRAAQLFEAVPQVERVEVERGADGFDTFRLGLAADVEVGESVA
                     EIARRHDWPIRELRRDDKSLEQVFRELTETDEEAAA"
     gene            1680..2402
                     /locus_tag="VD788_07090"
     CDS             1680..2402
                     /locus_tag="VD788_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV76073.1"
                     /translation="MNQVRSLAGRELRSYFNSPIAYVFLLVFVGAALFTFFNVNAFFS
                     RGRADLRGLFDAVPILMILLVPALTMRLWAEERKQGTIEVLLTLPVKEHELVAGKFLA
                     SWALLACGLALTLVLPITVASLGDLDWGPVVGGYFGALLLGGAYLAAGQFVSAATENQ
                     ILAFILALVMCLGLYGIGAEAFAGLFPDRTASLLRSVGTGSRFDSIARGVIDVRDLVY
                     YVSLMAIFLAACVATLRTRRWA"
     gene            2418..4592
                     /locus_tag="VD788_07095"
     CDS             2418..4592
                     /locus_tag="VD788_07095"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HXV76074.1"
                     /translation="MSERRTTYGSTLLTLALLALNVIAFNVLISYGTGPRLDLTEDRL
                     YSISPATERLLANLDEDLTILGYFSDRTHPKLAPLVPQVADMLDEYREMSNGRVRVII
                     TDPRDNEEQEQEANNRYGVTSTPFRLASKYETGIVNAYFALVVIYGDQYVRYGFDDLI
                     EIQPTPDGDVDVALRNLEYDLTRAIKKVVYGFRSTAELFERVDQPVKLTTIVSSETVP
                     EVFRPTVDAVRLAADELEQTGGERFEYEEIDPTGDPALEAQVYQRFGARPMTLGLFSD
                     EQFYLYGFLESDGRVEQVVLTEDGLSAATVREAIEDSMRRQTPGFLKTVGVVAPDPSL
                     PPEVLMQLQMQGQRVPQPPPEFEQVQAVLRADYQIESVDLTGSVPTDVDVLVVLKPKA
                     FDENEVFHLDQYLMRGGRVILCAARYEPQFSPSSGLTVTPVDTGLEDWLAHFGVRIPQ
                     TLVLDDQNQPLPIPEVTRTPLGMMQSWRMEPYPYLVEVSGDGLVNREIAARLDGVGIY
                     WGSPLELEQGAADGIETIEILKSSPRSWTDSDLSRVSYVDYEVPQEGTERHLLAVALS
                     GKFTSYFKENEPPAVEPPPGPDGDEAPGEPAARVVLEESPDTRLVVVGDAAFVSDLVA
                     RALALDSGFFRSNLAFVQNLIDWVNLDSEMLAIRSRGAGVARLERIERGTEVTIELAN
                     YLVPVGALALFGVYRYWRRRHVVPVVPGGARRTASKPRRAEG"
     gene            4589..5566
                     /locus_tag="VD788_07100"
     CDS             4589..5566
                     /locus_tag="VD788_07100"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HXV76075.1"
                     /translation="MSRGQWILLVLLVLQGGLLLIVAAPWSTGAGIDGTRVLVRELGT
                     VEPARIEIVEGDVTLSLQQQDGRWRIEQADGYPADASKVERLIGDLEQIEVRRPVVSG
                     SRYHEALKVTVDDNERRLRLWGSPDGEPAVELLIGSSPNYGVQHVRRRDEDRVYEVRG
                     LSPWDLRADAGSWIDTTLLEVAAADVVSLRITHGAEGFGLSREDGSEWHRSDGEAPVD
                     PAKVDRFLETIEGLRVSEPAGPAGPAEQRASFGLDEPAAVLELVHRPAENATETVTLR
                     IGRALDDDSGKRYAWVDGAEYAVVLSKWDAERVVDQRLESFAPDDDGDG"
     gene            complement(5521..6753)
                     /locus_tag="VD788_07105"
     CDS             complement(5521..6753)
                     /locus_tag="VD788_07105"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXV76076.1"
                     /translation="MNYRQLFGKLEQTLARIERTDDSVTLTSAILERVVEDFRDDLGL
                     TDGRFYVRRGDRLVLESEFPPSADHLGYEIPVSYPPVAELFERGFVLHEPDDPGVDSQ
                     IEDPLGVRTFAAIQVGPVRQQVLAFGLRDRSDREHVIYMLNTIRHAVNLKLRQQHLED
                     RVAEAREIQLSLLPSAAPRFHDYDVHGATRTAEEVGGDLYDFLPVSSRSLGIAIADSA
                     GHGLPAALQARDAIIGLRMGVEERLRLTATIEKLNRVVSQSALITRFITLFYGEVEPN
                     GNLVYCNAGHPPPLLLRDGRISELREGGLLLGPNPDARYERGYTMMAPGSVLLLYTDG
                     ISEATGRDGTMFGTKRLRALLRSRDWGSARELVEAAFAEVRAFSAVDPPADDQTVVAV
                     VRRPQPSPSSSGANDSSR"
     gene            6924..8087
                     /locus_tag="VD788_07110"
     CDS             6924..8087
                     /locus_tag="VD788_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV76077.1"
                     /translation="MTNELLTVPDHELIERAREVADRAMRPVAAKHDVEQTYPWEVQR
                     AIKEAGLAGVWIPKEYGGTGGGVLDLCLVIEEFSKACGGMGVAYAVNALGSFPIILSG
                     TETQKRRWLPGIAAGEKLIAFGLSEKEAGSDAGSMTTTAVRDGDEYVLNGEKKWNTGG
                     AVASLNTIFAVTTPGRGARGISGIVVENDAPGYRVGKHEDKMGIRCVPVVELHLEDCR
                     VPAENLLGGKEGLGFKHAMMTLDRARPGVAAQAVGLAQGAFDLAVEYTGKRKQFGQSI
                     SAFQGIQWMLADMATQIEAARQLVHAAARAADAGAKNLSKLSAMCKLFATDVAMKVTT
                     DAVQLFGGYGYVKDYPIEKYMRDAKITQIYEGTNQVQRLVIARTILREAASAV"
     gene            8397..9035
                     /locus_tag="VD788_07115"
     CDS             8397..9035
                     /locus_tag="VD788_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HXV76078.1"
                     /translation="MNDDRGPLSVRHLDAAVDLMGRNGRIAVLPVTGDSMLPSLREGQ
                     LIAVELLPDDPELGDLLVFRQHDYLVVHRFLGPATREDGRPWLRTRGDNVPTLDPVVE
                     RSRVRGRVIAFEKDGRWWGLRTGRARAWALAVALHDLSWAAAGVLARAAEARFRRAGL
                     SLPLGGWVASVDRLALAWAHRMLFRPLHRDGRSAPEAFSAPRRAPATTRGTR"
     gene            9095..11599
                     /gene="secA"
                     /locus_tag="VD788_07120"
                     /pseudo
     CDS             9095..11599
                     /gene="secA"
                     /locus_tag="VD788_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
     gene            11618..11827
                     /locus_tag="VD788_07125"
     CDS             11618..11827
                     /locus_tag="VD788_07125"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HXV76079.1"
                     /translation="MTHEEREREEQRRRAEQQQIFASASRSAAGVAARGGVQTVKRTA
                     AKVGRNDPCPCGSGKKFKKCHGTGA"
     gene            11880..>12615
                     /locus_tag="VD788_07130"
     CDS             11880..>12615
                     /locus_tag="VD788_07130"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HXV76080.1"
                     /translation="MNDSRFPEGLTFDDVLLTPAHSDVLPSQVTTRTRLTRKIWLNIP
                     LLSAAMDTVTESQLAIAIAQEGGIGVVHKNLPVEAQAEEVDKVKRSESGMIVDPITMR
                     PDQRIFEALELMSRYRISGVPVTDDEGRLVGILTNRDLRFCTHTDQSIRALMTSDNLV
                     TVPVGTTLEQAKELLHRHRIEKLPVVDGAFKLRGLITVKDIQKQIKYPNACKDELGRL
                     RVGAAVGTAPDTLERVAALVEAKVDVV"
BASE COUNT         2132 a         4125 c         4399 g         1959 t
ORIGIN      
        1 gccgtctcgg gcacgtcggc gttgatccac gtcatgttgt cggtgttgct cggcctggcc
       61 gcgctggccg tcggcgtgac gaccgcggag ctgatgggcc agaccctcga gcgcgccgcg
      121 tcggtcctgc tggtcgtgtt cggatcggtc tacgtgtggt gggcctggcg caaggggggc
      181 cacttccacc cggggggccg ccggctccac ggctcgcacg aacgggagga atgcgacggg
      241 cgcgagggag atggcaaccc cgagcacctg cactaccacg ccgataccgc gttgatcgcc
      301 gaccgcggcg cgtggtggct cgccttcgtc atcggggtta acccgtgcgt gctgatcctg
      361 ccggtgatct tcgccagcgc ccgcggcgga ccggcggccg tcgccgccgt gtgcgccgcc
      421 tatgccgtgc cgaccgccct gttgatggtc ggtctgtcgg cggccggcgt ggccggcggc
      481 gagaagctgt cgctgccgct ggctgcgcgt tacgccgaag tcgcgagcgg tctggtgatc
      541 gccgggctcg gggtgtttct gctggtggcg cacggctccc actgaccgct tcgagcggtt
      601 gacatccggc ggaaccgccg ctataagaac aacgtttttc cggcccggcg cgctcgtgcc
      661 cgggcatgtc ccgtgacgca cggtggcggg atcgagacga caagcacggt cgcggcccgc
      721 ggcggccgaa cggtggagac acggatgatc gaaattcgcg atctctggaa gaactacggc
      781 gaaacgcaag ccctgcgcgg cgtcggtttc tcgatccggc gcggcgaagt cgtcgggttg
      841 ttggggccga acggcgcggg caagacgacg acgatgaaaa tcgtggtcgg ctacctgaca
      901 cccagccgcg gcacggcgac cgtcgccggc atcgacgtcg ggcgagaccc gctcggggtc
      961 cagcgactga tcggctacct gccggagaac gcgccgctct actccgacat gctcgcgcag
     1021 gagtacctcg agttcatggc cgacgttcgg caactcgccg ccgtcgagcg ccgcgcccgg
     1081 atcgagcaag tcgtcgacga gtgcgcgatc cagtcggtat tgacccgccc gatcggccac
     1141 ctgtccaagg gcttccggca gcgcgtcggg ctcgcgtcga cgctgcttca cgacccgccg
     1201 atcctgatcc tcgacgagcc gaccggcggc ctcgacccga accagatcgt cgaggtgcgg
     1261 gagttgatcc ggcgactggg tcggacgaag acgatcatcc tctcgaccca catcctcagc
     1321 gaggtcgaga tgagttgcgg gcgcgccgtg atcgtgatcg acggacgggt gcgcgccgac
     1381 ggctcgctcg acgagctgac gcgggccaag gctcagctgg tcacggtcaa gacgggggat
     1441 ccttcccgcg cggcgcagct gttcgaggcg gtgccgcagg tcgagcgtgt cgaggtcgag
     1501 cgcggcgcgg acgggttcga cacgttccgg ctcgggctcg ccgccgacgt cgaggtcggc
     1561 gagtcggtcg cggagatcgc gcgccgccac gactggccga tccgcgagct gcgacgcgac
     1621 gacaagtcgc tcgagcaggt attccgcgaa ttgaccgaaa cggacgagga ggccgcggca
     1681 tgaatcaggt tcgctcgttg gccggccgtg agttgcgctc gtacttcaac tcgccgatcg
     1741 cctacgtctt cctgctcgtc ttcgtcggtg cggcgctgtt caccttcttc aacgtcaacg
     1801 ccttcttctc gcgcgggcgc gccgatctgc gcgggctgtt cgacgcggtg ccgatcctga
     1861 tgatcctgct cgttccggcg ctgacgatgc ggctgtgggc cgaggagcgc aagcagggga
     1921 cgatcgaggt gctattgacg cttccggtca aggaacacga gctggtcgcc ggcaagttcc
     1981 tcgcgagctg ggcgctgttg gcctgcggtt tggcgttgac gctggtcctg ccgatcaccg
     2041 tcgcgtcgct cggcgacctc gattggggac cggtcgtcgg cggatacttc ggcgcgctgt
     2101 tgctcggcgg ggcctacctc gccgccggcc agttcgtctc ggcggcgacc gagaatcaga
     2161 tcctcgcctt catcctggca ctggtcatgt gtctcgggct ctacggcatc ggggcggagg
     2221 cgttcgccgg tctgtttccc gatcgcacgg cgagcctgct ccgatcggtc ggaacgggca
     2281 gccggttcga cagcatcgcg cgtggggtga tcgatgtacg ggacctcgtc tactacgtgt
     2341 cgctgatggc gatcttcctc gcggcgtgcg tcgccaccct gcgcacccgg cgctgggcct
     2401 gagtagagga gcgaaaaatg agcgaacgtc gcacgaccta cggctcgacg ctgctgacgc
     2461 tggcgttgct cgcgctgaac gtgatcgcgt tcaacgtact gatctcgtac gggaccggcc
     2521 cacggctcga tctgaccgag gaccggctgt actcgatctc gccggccacc gagcggctgc
     2581 tggccaatct cgacgaggat ctgacgattc tcggttattt ctccgaccgc acgcatccca
     2641 agctggcgcc gctcgtgccc caggtcgcgg acatgctcga cgagtaccgc gagatgtcga
     2701 acggcagggt gcgcgtgatc atcaccgacc cgcgcgacaa cgaggagcag gagcaggaag
     2761 cgaacaaccg ctacggcgta accagcacgc cgtttcggct ggcgtccaag tacgagaccg
     2821 ggatcgtcaa cgcctatttc gcactggtcg tgatctacgg tgaccagtac gtgcgctacg
     2881 gtttcgacga cctgatcgag atccagccga ccccggacgg cgacgtcgac gtcgccttgc
     2941 gcaacctgga gtacgacctg acccgcgcga tcaagaaggt ggtctacggc ttccgcagca
     3001 ccgccgagct gttcgagcgc gtcgatcagc ccgtgaaatt gaccacgatc gtctcgagcg
     3061 agacggtccc ggaggtgttt cgaccgaccg tcgacgcggt gcggctggcc gccgacgagc
     3121 tcgagcagac cggcggcgag aggttcgagt acgaggagat cgatccgacc ggcgacccgg
     3181 cgctcgaggc gcaggtctac cagcgtttcg gcgcgcggcc gatgacgctc ggactgttct
     3241 ccgacgagca gttctacctc tacgggttcc tcgagtccga cggccgcgtc gaacaggttg
     3301 tgctgacgga ggacggcctg tcggcggcga cggtccgcga ggcgatcgaa gattcgatgc
     3361 gccggcagac tccagggttc ctcaagacgg tcggcgtggt cgcccccgat ccgtcgctgc
     3421 cgcccgaggt actgatgcag ctgcagatgc agggccagcg cgtgccgcag ccaccccccg
     3481 agttcgagca ggtgcaggcc gtcctgcgcg ccgactacca gatcgagagc gtcgatctga
     3541 cgggcagcgt cccgaccgac gtcgacgtgc tcgtcgtgct caagcccaag gccttcgacg
     3601 agaacgaagt gttccacctc gaccagtacc tgatgcgagg tggccgggtg atcctctgcg
     3661 ccgcgcgcta cgaaccgcag ttcagcccga gctcggggct gacggtcacg ccggtcgata
     3721 ccggtctcga ggactggctc gcccacttcg gcgtccgcat cccccagaca ctcgtgctcg
     3781 acgaccagaa tcagccgctg ccgattccgg aggtgacgcg cactccgctg ggcatgatgc
     3841 agtcgtggcg gatggagccg tacccctatc tcgtcgaagt cagcggcgat ggcttggtca
     3901 atcgcgagat tgccgcgcgg ctcgacgggg tcggcatcta ctggggcagc ccgctcgagc
     3961 tcgagcaagg tgcggccgac ggcatcgaga cgatcgagat cctcaagtct tcgccccgct
     4021 cgtggaccga ttccgacctg tcgcgcgtct cgtacgtcga ctacgaggtg ccgcaggagg
     4081 ggaccgagcg ccacctgctc gcggtggcgc tcagcgggaa gttcaccagc tacttcaagg
     4141 agaacgaacc gccggccgtc gagccgccgc ccggccccga cggtgacgag gcgccgggcg
     4201 agccggcggc gcgcgtcgtg ctcgaggaat cgcccgacac gcggctggtc gtcgtcggcg
     4261 acgccgcgtt cgtcagcgac ctggtggcgc gcgcgctggc gctcgacagc ggcttcttcc
     4321 gctcgaacct ggccttcgtc cagaacctga tcgactgggt caacctcgac agcgagatgc
     4381 tggcgatccg ctcgcgtggc gccggcgtgg cgcgcctcga gcggatcgaa cgcggtacgg
     4441 aggtcacgat cgagctggcg aactacctcg taccggtagg ggcgttggcg ttgttcgggg
     4501 tctatcgcta ctggcgccgg cggcacgtgg tgccggtcgt gccgggcggc gcgcgccgca
     4561 cggcgagcaa gccgcggagg gccgagggat gagtcgtggc cagtggatac tgctcgttct
     4621 gctcgtgctg cagggtggac tgctgctgat cgtcgccgcg ccgtggtcga ccggggcagg
     4681 gatcgacggc acgcgggtgc tggttcgcga actcgggacg gtcgagccgg cgagaatcga
     4741 gatcgtcgag ggcgacgtta cgctctcgct tcagcagcag gacggacgct ggcgtatcga
     4801 gcaggccgac ggctacccgg ccgacgccag caaagtcgag cggctgatcg gcgacctcga
     4861 gcagatcgag gtgcgccgcc cggtggtcag cggcagccgc tatcacgagg cgctcaaggt
     4921 cacggtcgat gacaacgagc ggcgcctgcg cctgtgggga tcaccggacg gtgagccggc
     4981 ggtcgagctg ctgatcggca gctcacccaa ctacggcgtc cagcacgtga ggcggcgcga
     5041 cgaggaccgg gtctacgaag tgcgcgggct cagtccgtgg gacctgcgcg ccgacgccgg
     5101 ctcctggatc gacacgacgc tgctcgaggt ggcggcggcc gacgtcgtct cgctgcggat
     5161 cacccacggc gcggagggtt tcgggctgtc gcgcgaggat gggagcgagt ggcatcgatc
     5221 cgacggcgag gcaccggtcg acccggcgaa ggtcgaccgc ttcctcgaga cgatcgaagg
     5281 cctgcgtgtc agcgagccgg ccgggccggc cgggccggcc gagcaacggg cctcgttcgg
     5341 tctcgacgaa ccggcggcgg tgctggagct cgtgcaccgg cccgccgaga atgcgaccga
     5401 gaccgtcacc ctgcgcatcg gtcgcgccct cgacgacgac agcggcaaac gctacgcctg
     5461 ggtcgatggc gcggagtacg cggtcgtcct gtcgaaatgg gacgccgagc gcgtcgtcga
     5521 tcagcgactc gagtcgttcg cccccgacga cgacggcgac ggctgaggac ggcggacgac
     5581 ggcgacgacg gtctgatcgt cggcgggcgg atcgacggcg gagaacgccc ggacctcggc
     5641 gaaagcggct tcgacgagct cgcgcgccga gccccaatcg cgcgagcgca gcagcgcgcg
     5701 cagccgcttc gtgccgaaca tcgttccgtc gcgcccggtg gcctcggaga tcccgtcggt
     5761 gtacagcagc aacaccgacc cgggcgccat catcgtgtag ccccgctcgt atctcgcgtc
     5821 gggattgggt ccgagcagca accccccctc ccgcagctcg ctgatccgtc cgtcgcgcaa
     5881 gagcaacggc ggtgggtgac cggcgttgca atagaccagg ttgccgtttg gctcgacctc
     5941 gccgtaaaac agcgtgatga agcgcgtgat caacgccgac tgactgacga cgcggttcag
     6001 cttctcgatc gtcgcggtca agcgcagccg ctcctcgacg cccatccgca gaccgatgat
     6061 cgcatcgcgc gcctgcagcg cggcgggcaa cccgtgaccg gcagagtcgg cgatcgcgat
     6121 acccaacgag cgggaagaaa ccggcaagaa gtcgtagagg tccccgccga cctcctccgc
     6181 ggtgcgcgtc gccccgtgca cgtcgtagtc gtggaaccgc ggcgcggccg acggcagcag
     6241 cgacagctgg atctcgcgcg cctcggccac ccggtcctcg aggtgctgct ggcgcagctt
     6301 caagttgacc gcgtggcgga tcgtattgag catgtagatc acgtgctcgc ggtccgagcg
     6361 gtcgcgcagg ccgaaggcga ggacttgctg ccggaccggt ccgacctgga tcgcggcgaa
     6421 ggtgcgcacg ccgagcggat cctcgatctg gctgtcgacg ccggggtcgt ccggctcgtg
     6481 aagcacgaag ccgcgctcga acagctccgc caccggaggg taggagacgg ggatctcgta
     6541 gcccaggtgg tcggccgatg gtggaaactc gctctcgagc accaggcggt cgccgcggcg
     6601 cacgtagaac cggccgtcgg tcagaccgag gtcgtcgcgg aagtcctcga cgacgcgttc
     6661 caggatggcc gaggtcaacg tgacggaatc gtcggttcgc tcgattcggg ccagggtctg
     6721 ttccagtttg ccgaagagtt ggcggtagtt cacgtctcgg ctcgcgctcg gctgtcggcg
     6781 ggcgctccgc aggccggcac gcgacactag caaatcgcga cggcgttcgt caaagggcca
     6841 acttccacga cccgaccgac gatggaatcg gcgccccgcg gatggtagag tgacggctct
     6901 ccttctcgtt catcgaggtg ccgatgacca acgagttgct gaccgttccc gaccacgaac
     6961 tgatcgagcg ggcgcgcgag gtggccgacc gcgcgatgcg tccggtcgcg gccaagcatg
     7021 acgtcgagca gacctacccg tgggaggtac agcgggcgat caaggaggcc ggtctggcgg
     7081 gagtgtggat ccccaaggag tacggtggga ccggcggcgg cgtgctcgac ctgtgcctgg
     7141 tgatcgagga gttctcgaag gcctgcggcg ggatgggcgt ggcctacgcc gtcaacgcgc
     7201 tggggtcgtt tccgatcatc ctgtcgggca ccgaaacgca gaagcggcgc tggctaccgg
     7261 gaatcgccgc cggcgagaag ctgatcgcgt tcggcctgtc cgagaaggag gccggttcgg
     7321 atgccggctc gatgacgacg acggccgtgc gcgacggcga cgagtacgtg ctcaacggcg
     7381 agaagaagtg gaacaccggc ggcgcggtcg cctcgctcaa cacgatcttc gccgtcacca
     7441 cgccggggcg cggtgcccgc gggatctccg gtatcgtcgt cgagaacgac gcgccgggct
     7501 accgcgtcgg caagcacgaa gacaagatgg gcatccggtg cgtcccggtc gtcgagctgc
     7561 acctcgagga ctgccgggta ccggcggaga acctgctggg cggcaaggaa gggctcggct
     7621 tcaagcacgc gatgatgacg ctcgaccgcg cgcggcccgg cgtcgccgcg caggccgtcg
     7681 gcctggcgca gggcgcgttc gatctggcgg tggaatacac cggcaaacgc aagcagttcg
     7741 gccagtcgat cagcgccttc caagggatcc agtggatgct ggccgacatg gcgacgcaga
     7801 tcgaggcggc gcgccagctg gtccacgccg cggcccgggc ggccgacgcc ggcgcgaaga
     7861 acctcagcaa gctgtcggcg atgtgcaagc tgttcgcgac cgacgtcgcg atgaaggtca
     7921 cgaccgacgc cgtgcagctg ttcggtggtt acggctacgt caaggactat ccgatcgaga
     7981 agtacatgcg cgacgcgaag atcacgcaga tctacgaggg cacgaaccaa gtccagcggc
     8041 tggtcatcgc gcggacgatc ctgcgcgagg cggcgagcgc cgtctagcgc gccgcgtcat
     8101 gccacgccgc cgatctccga cccgccgtcc gagccgatgt cagaacggcg cgacgagtgt
     8161 caccacgcgt cagcatggcg tccactctgt ggcacacccc ggggggcggc aatcccgaca
     8221 acgtcccgga aactcgcgaa aagtagcgac aaatcgccac gggtccgagc tggcatcgtg
     8281 cttgctctat cggtgggcag gacatgacct cctcctggtc agtgtcgaac cccctccggg
     8341 cctgatggtc atgccccccc gcgggcccgg agggggttga cttttttctg catgccgtga
     8401 acgacgatcg cggtccactc tccgtccgac atctcgatgc ggcggtcgac ctgatgggtc
     8461 gcaacggccg gatcgccgtg ctcccggtca ccggcgacag catgctgccg tcgctgcgcg
     8521 agggtcagct gatcgccgtc gagctcttgc ccgacgaccc cgaactgggc gacctgttgg
     8581 tcttcagaca acacgactat ctcgtcgtgc accgctttct cggaccggcg acgcgcgagg
     8641 acggccggcc gtggctgcgc acgcgcggcg acaacgtccc cacgctcgat ccggtcgtcg
     8701 agcgctcgcg ggtgcgcggg cgtgtgatcg cgttcgagaa ggacgggcgc tggtggggcc
     8761 tgcgcaccgg ccgtgcgcgc gcgtgggccc tggcggtcgc gctgcacgac ctgtcgtggg
     8821 ccgcggccgg tgtgctggcg cgtgcggcgg aagcgcggtt ccgccgcgcg ggtctctcgc
     8881 ttcctctcgg aggttgggtc gcgtcggtcg atcgcctggc gctggcctgg gcccatcgga
     8941 tgttgttccg cccgctgcac cgcgacggtc gaagcgcgcc cgaggcgttc agcgccccgc
     9001 gacgcgcgcc ggcgacgacg cggggcaccc gctgagcgcc tgctatactt tccgaccgga
     9061 tcccgcgccc ccgcgcgcga caggctgaac gaaaatgatc aattccctgc tgaccaaggt
     9121 cgtcggctcg aagaacgatc ggatgctcaa gaagatgcgt ccgctggtcg agcagatcaa
     9181 cgccctcgag ccgtcgatcg aggcgttgcc cgacgaccgg ctgcgcgaca agacggccga
     9241 gttccgccag cgcctcgagg ccggacagcc gctcgacgac ctgctgcccg aggcgttcgc
     9301 cgtcgtccgc gaaaccggtc gccgcgtgat ggagatgcgg catttcgacg tacagctgat
     9361 cggcggcctg gtgttgcatc gcgggatgat cgccgagatg aagaccggcg aaggaaaaac
     9421 gctcgtcgcg acgctccccg gctacctcaa cgcactgacc gacaacggcg tgcacgtcgt
     9481 caccgtcaac gactacctgg cacgccgcga ctcggagtgg atgggccgga tctaccgctt
     9541 tctcggcttg tccgtcggcg tgatccaaca cgacctcaac gacaagcagc ggcaggaagc
     9601 ctatgccgcc gacgtgacct acggcacgaa caacgagttc ggcttcgact acctgcgcga
     9661 caacatgaag ttcacgctcg aatcgatggt acagcgcggc caccacttcg cgatcgtcga
     9721 cgaggtggac tcgatcctga tcgacgaggc gcgcacgccg ctgatcatca gcgggcccgc
     9781 cgaggagtcg accgacaagt actcccgcat caaccgcatc atccctcggc tggaaaaggg
     9841 ggcggtgatc cagggcggca aggactacga agtcgaacac accggcgatt acgtcgtcga
     9901 cgagaaggcc cggaccgtca cgctgtcgga acgcggcgtc gagcgctgcc aggagatgct
     9961 gggcgtcgac aatctgtacg atccggccca catggacctg gtgcaccacg tcaaccaggc
    10021 gttgaaagcg cacgtgttgt tcaagcgcga cgtcgattac atgatcaagg acggccaggt
    10081 ggtcatcgtc gacgagttca ccggcaggct gatgcccggg cggcgttgga gcgacggatt
    10141 gcaccaagcg gtggaggcga aggagggcgt caaaatcgag cgcgagaacc agacgctggc
    10201 caccatcacc ttccaaaact acttccgcat gtacgacaaa ctgagcggga tgaccggaac
    10261 ggcggacacc gaggccgtcg agttcgacaa gatctatgaa ctcgaggtca gcgtcgtgcc
    10321 gaccaaccgc gagctgatcc gcgtcgagta ccccgacgtg atctaccgca ccgacacgga
    10381 gaagtggacc gcggtggtgg aggacatcga ggagagccag cagcgcggcc aaccggtgct
    10441 ggtcggcacg gtgtcgatcg agaaatcgga gacgctgtcg ggcctgctgc gcaagcgcgg
    10501 catcaagcac gtcgtgctga acgccaagta ccacgagatg gaggccgaga tcgtcgcgca
    10561 ggccggccgc gtcggggcgg tgacgatcgc gaccaacatg gccggccgcg gtaccgacat
    10621 cctgctcggc ggcaaccccg agtttctcgc gttgcgcggg ctgagcgagc gcggcgtcaa
    10681 gccggccgag gtctcacccg agcgctggga cgaggagttc gccaaagagc tcgcgctgtc
    10741 gaaggcccgt tgccgaaagg agcgcgagac cgtcatcgac gccggcggtc tgttcatcct
    10801 cggaaccgaa cggcacgaat cgcgccggat cgacaaccag ctgcgcggtc gcgccggtcg
    10861 gcagggtgac ccgggcgcct cgcgctttta cctgtcgctc gacgacgacc tgatgcggat
    10921 cttcgccggc gaccgcttca aggcggcgat gaagaacctc ggcatggaag agaacgtgcc
    10981 gatcgagtcg aggatggtca gccgggcgat cgagcgcgca caaaagcagg tcgaacagcg
    11041 caacttcgag atccgcaagc acctgctcga gtacgacgac gtcaacaaca agcagcgcaa
    11101 cgaggtctac ggcttgagac gcggcttgct cgagggcaag gagcagaagg aatacgtgct
    11161 ggagaaggcg cgcgacatcc tcgacttcct cgtcggcgac tacctggccc agcacaagtc
    11221 gccggccgat tgggaggtcg ccggattccg ccagcagctg gtccacttct tcgcgctcga
    11281 tcccgacgcc agcggagtcg acttcgccac gtcgaatctg gaccaggtgt cgcaacggtt
    11341 gtggcaagcg ctgggcgaca agtacgacgc caaggaagaa aaggtcggcg ccgagttgat
    11401 gcgacactac tagcgccaca tcctgttgca gatcatcgac tccgcctgga aggaccacct
    11461 gctggcgatg gaccatctca aggagggtat cgggctgcgg gcctacggcc aacgcgaccc
    11521 gctggtcgag tacaagaaag aatcgttcga gatgttcggc cagatgaagg agcgcatcga
    11581 gaacgacgcc gtgcgtttcc tgttcatgct cgagccgatg acccacgagg agcgcgagcg
    11641 cgaagagcag cgccgacgcg ccgagcagca gcagatcttt gcctccgcct cgcgctcggc
    11701 cgcgggggtc gccgcgcgcg gcggcgtgca gaccgtcaag cgcacggcgg cgaaggtcgg
    11761 tcgtaatgat ccgtgcccgt gcgggtccgg caagaagttc aagaagtgcc acgggacggg
    11821 cgcgtgaagc gcggcgtcgt ttcgtctaga atggcgtgat cgctggccgg agagggcgga
    11881 tgaacgactc gcgcttcccc gagggcttga ccttcgacga cgtgctgctg acccccgccc
    11941 acagcgacgt gctgccgtct caggtgacga cgcgcacgcg cctgacgcga aaaatctggc
    12001 tcaacattcc gttgctgtcg gcggcgatgg acaccgtcac cgaatcgcag ctcgcgatcg
    12061 cgatcgccca ggagggcggc atcggggtcg tgcacaagaa cctaccggtc gaggcccagg
    12121 ccgaagaggt cgacaaggtc aaacggtccg agtcgggcat gatcgtcgat ccgatcacga
    12181 tgcgtcccga ccagcgtatc ttcgaggcgc tcgagctgat gagccgctat cgcatctcgg
    12241 gcgtgccggt gacggacgac gaaggccgtc tggtcggtat cctgaccaac cgcgaccttc
    12301 gcttctgcac gcataccgac cagtcgatcc gggcgttgat gacctccgac aacctggtca
    12361 ccgtcccggt cgggacgacg ctcgagcagg ccaaggagct gcttcaccgg caccgcatcg
    12421 agaagctccc ggtcgtcgac ggtgcgttca agctgcgcgg gttgatcacg gtcaaggaca
    12481 tccagaagca gatcaagtac ccgaacgcgt gcaaggacga gctggggcgg ctgcgcgtgg
    12541 gggcggccgt cggcaccgcg cccgacacgc tcgagcgggt ggcggcgctc gtcgaggcca
    12601 aggtggacgt ggtgg
//
LOCUS       DASPGN010000244         5781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_744691, whole genome shotgun sequence.
ACCESSION   DASPGN010000244 DASPGN010000000
VERSION     DASPGN010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744691"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..691
                     /locus_tag="VD788_07135"
     CDS             206..691
                     /locus_tag="VD788_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76081.1"
                     /translation="MRIRLLVQAATTVVCLQGVVIPAESPALQGRYFFSDATVADDRV
                     TVTLNLWLRNPVRSSIDGVSLTLHATNGDETLATHAPVSFADGEVLPFRWPVEIPAAE
                     YELWRQGARPKVEFVDPSEGESAASRPVDLAFDIFELPPPEIDESDAVDPSRIPTQRE
                     N"
     gene            693..5573
                     /locus_tag="VD788_07140"
     CDS             693..5573
                     /locus_tag="VD788_07140"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HXV76082.1"
                     /translation="MPGGLRALALAVLLVTASGSVVASWVGEAIGGGGPDGLPALESN
                     LAVPSAVVIGPDGMPWIAARESGQILRLTPSGTIEIVFQGEVVDSVGGVIDDPWDLAF
                     GPNGELAVGERVVTLLLPELSAAPRVVHGGSAVDFGPAGELYLVDFQRTQISRFDPQS
                     GSLEPFAGCPGSIPFCHGSAEGSPALDYRLTSIADIATDAGGNLYIAVPADRILRVDA
                     SDQTIRTVIGNGQPGFAGDDGPAASALVDDPTDIAIAPNGDLYFVDSRNFRIRRIDAA
                     TGTVTTVAGNGVNGYAGDGGPAIDASFPAISGLAVNAEHDLFVTANLRLRKIDAATGI
                     IDTVAGNGTIFFSGDGYPAHRASISGGATGVDADGNVFVADTFNHRVRRIDASTGIIT
                     TYAGTGASTTGIDDDGLQATQVVLPSPVGLSFDAEQNLYVRVDGGVRRIDRDTGIVTT
                     MPAGSLGVVDDLGRDYRVDLASIYRIDPATGTQEHVAGNGTICGVTTGGPASQTSVTA
                     PRSIRFDAAGNIYFVELRLFCDNLQSSSVVRRISHDLQRLDTLTGGVLPSFDHPLNLT
                     FGPAIGVPSLAHAIAVEPGGAVWFTSWAFTARLDPRTGLLERVLGPGGPYAISELLSR
                     PESDLDSLDAEGLSIGPDRSLFLVSAKRVYRVTEGGSPPVAVAAAPASALCAGAHGAT
                     VTLDGSSSHDPDDLDSASDSIANHWWYEDYGTPNERLIGKGAVAEAELSIGSHPITLL
                     VRDVRGNEATDEIEIVVTGTGDADGDLIDDCADNCSMMANPTQLDSDANRVGDACDSC
                     WSLGETDGDGDGVCTALDNCPEVANSGQADRDGDGFGDSCDVCPVTFNDEPSASVRCV
                     TAREPVGDCRRVVADVNRPGSIGRVILTGFDRSVPDKIEFHWRGVCSPRTFELLLNGT
                     LLDSWFSYTACSPAFTYSDPALIQSAWRTDGENELRLRTIDGPTRVVRAYATVTAGQR
                     SQTACFVDATGSDCLIPLESGSLRTVGFSDATVLFSNRFAAVSSVVEQPYGPDPLSVS
                     FDLDTSFDGHRLCVSSTVPDAPPLLGISTTGELYEVNPLTAELDRRGFLPIVPVDADY
                     DVATGQAFAASGAEFGVRFDVRDGSKIGQVLNTPFGYENVEFAFGDLIATYDFYWPQE
                     GYAHRSDLLDLDNQTIGDTPVTFPDQRNPLTGFTHDESSGIFWGVIRDIVQNFDPLTG
                     ASRTLGYYPSRQFSGIELGPDGFLYLVSEPYSNEPPRLFRVGRDDPRLVAVGDLDLPP
                     TWTLARLTTGGRPGSVDCIDVDSQGETRLAINDTCGPPTARIAPVAPVECVSAAGAAV
                     TLDGSSSTDPNSTPGTQDALVSYEWYLDRGGPQELYLGSGAVLTTTLPFGLHQVTLVV
                     GNAYDERSTATIPVEVVDTQLPTLGLGLTPATLWPPNHDMVEIEATPDASDNCGPPAV
                     RLVSIGSSEPDDAPGGGDGTTTNDMRNAEIGVADTGFALRAERAGDGVGRVYEVTYEA
                     EDRGGLKVTATRRVLVPHSLGGATEPLLIRLHSEASGTTIEWDDVGGAVYYNVVRGDL
                     AAPSLLDSVVQLGSVDCIASGLVANSTAGYEDAGTPQPNAVFYYLAEYHDGWRASGYG
                     SESVAGPRSLSVGDCD"
BASE COUNT          906 a         1973 c         1907 g          995 t
ORIGIN      
        1 acctcgaaag cggtcgtccc agagccatga tgcccctcct cgctctcaaa acaccgggca
       61 tcatgttacc acacttatgt cacgtatcta tggaacagga cactaggtac tgtcctatcg
      121 aattcaggac acgacccggc cctggctcct gttgacgtgc cgggccgatc acggtataca
      181 ggtcgaaagc ctcacggagt aacagatgcg catccgactc ctggtccagg ccgcgaccac
      241 ggtagtctgt ttgcagggag tggtaatccc cgccgaatcc ccggcattgc agggacggta
      301 tttcttctcc gacgcgacgg tggccgacga tcgcgtgacg gtgacgctca acctgtggct
      361 gcggaatccc gtccgctcgt cgatcgacgg tgtcagcttg accctgcatg cgacgaatgg
      421 tgacgagacg ctcgcaacgc acgcaccggt gagcttcgcc gacggggagg tcctcccgtt
      481 tcgttggccg gtcgagatcc cggcggccga gtacgagctg tggcgtcaag gcgcgcgacc
      541 caaggtcgag ttcgtcgacc cgtcggaggg cgagtccgcg gcgtcgcgac cggtcgatct
      601 cgcgttcgac atcttcgagc ttccgccacc cgagatcgac gagtcggacg cggtcgatcc
      661 atcccgcatc ccgacccagc gggagaactg agatgccggg cggccttcgc gcgctcgcgc
      721 tcgcggtcct gctcgtcacg gccagtggtt ccgtcgtcgc gtcctgggtc ggcgaagcga
      781 tcggcggagg cggccccgac ggtttgccgg cgctcgaatc gaacctggcc gtgccgtccg
      841 ccgtcgtgat cggccccgat ggaatgccgt ggatcgccgc gcgagagtcg ggtcagattc
      901 ttcggttgac tccctccggg acgatcgaga tcgtgtttca gggcgaagtg gtcgacagcg
      961 tcggcggcgt catcgacgat ccgtgggacc tcgcgttcgg tcccaacggg gagctggcgg
     1021 taggcgagcg agttgtgacg cttctcctgc ccgagctttc tgccgcgccc cgggtggtcc
     1081 acggcggatc ggcggtcgac ttcgggccgg cgggcgagct ctatttggtc gatttccaac
     1141 gtacgcagat ctcccggttc gacccgcagt ccggctccct cgagccgttc gccggatgcc
     1201 ccgggtcgat cccgttttgc catggctcgg ccgaaggctc gcccgctctc gattaccgtc
     1261 tgacgtcgat cgccgacatc gcgaccgatg ccgggggaaa cctctacatc gcggtccccg
     1321 ctgatcgcat cctgcgggtc gacgcgtcgg atcagacgat tcgaaccgtg atcggcaacg
     1381 gtcagccggg gttcgccggc gacgacggac cggccgcatc ggccctggtc gacgatccga
     1441 ccgatatcgc gatcgcgccc aacggcgatc tgtacttcgt cgactcgcgc aatttccgga
     1501 tccgacggat cgacgccgcg accggcacgg tgacgacggt tgccgggaac ggcgtcaacg
     1561 gctacgccgg tgacggggga ccggcgatcg atgcgtcgtt cccggcgatt tccggtttgg
     1621 cggtcaacgc cgagcacgac ctgttcgtca ccgcaaatct gaggctgcgc aagatcgacg
     1681 cggcgactgg aatcatcgac acggtcgccg ggaacggcac gatcttcttc agcggcgacg
     1741 gctacccggc gcatcgtgcg tcgatatccg gcggcgccac gggcgtcgat gccgacggca
     1801 acgtcttcgt cgcggatacg ttcaaccacc gcgttcgtcg gatcgacgca tcgaccggga
     1861 tcatcacgac ctacgccggg accggcgcat cgaccacggg gatcgacgac gacggactgc
     1921 aggcgacgca ggtcgtcctg ccgagccccg tcggtctctc gttcgatgcg gagcagaacc
     1981 tctacgtgcg cgtcgacggc ggcgttcggc ggatcgaccg ggacaccggg atcgtgacga
     2041 ccatgccggc cggctcgctc ggagtggtcg acgacctcgg ccgggactat cgcgtcgacc
     2101 tcgccagcat ctatcgcatc gacccggcca cggggaccca agagcacgtc gccggcaacg
     2161 ggacgatctg tggcgtgacc accggtgggc ccgcgtcgca gacctcggtg accgctccga
     2221 ggagcatccg attcgacgcg gccgggaaca tctacttcgt cgaactccgg ctgttctgcg
     2281 acaacctgca gtcgagttct gtcgtgcgcc ggatctcgca cgatctccaa cgtctggaca
     2341 ccttgaccgg tggagtcctc ccgtcctttg atcacccgct caacctcact tttggaccgg
     2401 ctatcggcgt tccgagcctc gcacacgcga tcgccgtcga gccgggcggg gcggtctggt
     2461 tcaccagttg ggccttcacc gcgcgactcg acccgcggac cggactgctc gaacgcgttc
     2521 tcggacccgg cgggccctac gcgatctccg aacttctttc ccgacccgag tccgacctcg
     2581 acagtttgga cgcagagggc ttgtcgatcg gccccgaccg gtcgctgttt ctggtctccg
     2641 cgaagcgggt ctaccgagtc accgagggtg ggtctcctcc ggttgccgtc gcggcggctc
     2701 cggcgtcggc tctctgcgcc ggtgcccacg gcgcgacggt cacgctcgac gggtcgtcgt
     2761 cccacgaccc ggacgacctc gactcggcgt ccgattcgat cgcgaaccac tggtggtacg
     2821 aggactacgg gacaccgaac gagcggctca tcgggaaagg cgccgtcgcc gaagccgagt
     2881 tgtcgatcgg ctcgcatccc atcacgttgt tggtccgcga cgtccgcgga aacgaggcga
     2941 cggacgagat cgagatcgtg gtgaccggga ccggcgacgc cgacggcgat ctgatcgacg
     3001 attgcgccga caactgctcg atgatggcga acccgacaca gctcgactcc gacgccaacc
     3061 gggtggggga cgcctgcgac tcctgctggt cgctcggcga aaccgacggc gatggcgacg
     3121 gggtctgtac cgctctcgac aactgtcccg aggtcgccaa ttccggccag gccgatcgcg
     3181 acggcgacgg cttcggcgac tcgtgcgatg tctgccccgt gaccttcaac gacgagccga
     3241 gtgcgagcgt gcggtgcgtc accgcccgcg agccggtcgg cgactgccgt cgcgtcgtcg
     3301 ccgacgtgaa ccgcccgggt tcgatcggac gtgtgattct caccggtttc gaccgttcgg
     3361 tcccggacaa gatcgagttt cactggaggg gggtctgctc accgcggacg ttcgagctgc
     3421 tgttgaacgg aaccctgctc gattcgtggt tcagctacac cgcgtgctcc ccggcgttca
     3481 cctattcgga cccggcgctg atccagtccg cttggcgcac cgacggagaa aacgagctgc
     3541 gactgcggac gatcgacgga cccacgcggg tggtccgcgc ttacgcgacg gtcaccgcgg
     3601 gccaacgcag tcaaaccgcg tgcttcgtcg atgcaacggg ctcggattgc ttgatcccgc
     3661 tcgagtccgg ttcgctcagg acggtcggct tttctgacgc cacggtcctc ttttcgaatc
     3721 ggttcgccgc cgtgtcgtcc gtggtcgagc agccctacgg gcccgacccg ctgtcggtct
     3781 cgttcgatct cgacacgtcg ttcgacggcc accgcctgtg tgtgagctcg accgtgccgg
     3841 atgcccctcc gcttctcggc atctccacca caggtgagct gtatgaagtg aacccgctca
     3901 ccgccgagct ggatcggcga ggtttcctcc cgatcgtgcc ggtcgacgcc gactatgacg
     3961 tagccaccgg gcaggcgttc gcggcatcgg gtgccgagtt cggcgtgcgg ttcgacgtgc
     4021 gtgacgggtc gaagatcggt caggtactca acacaccgtt cggctacgag aacgtcgagt
     4081 tcgctttcgg cgatttgatc gcgacctacg acttctactg gccccaggaa ggctacgccc
     4141 accggtcgga cttgctcgac ctcgacaatc agacaatcgg cgacaccccg gtcacgttcc
     4201 cggatcagcg caatccgctg accggcttca cgcatgacga gtcgagcgga atcttctggg
     4261 gagtgatacg cgacatcgtc cagaacttcg atccgctgac gggcgcgtcg aggacactcg
     4321 gctactaccc gtcgcgccaa ttcagcggta tcgagctcgg tccggacggt tttctctacc
     4381 tggtcagcga gccctactcg aacgagccgc cgcggctgtt ccgcgtgggt cgcgacgacc
     4441 cgcgactcgt cgccgtcggt gacctcgacc tcccgccgac gtggacgctc gcgaggctga
     4501 cgacaggagg cagaccgggc tcggtcgact gtatcgacgt cgattcccag ggggagacgc
     4561 gactcgcgat caacgacacg tgcggtccgc cgaccgcgcg catcgcgccg gtcgccccgg
     4621 tcgagtgcgt ctcggccgcc ggggccgcgg tcacgctcga cgggtcatcg tcgaccgacc
     4681 ccaactcgac cccgggcacg caggacgcgc tcgtctccta cgagtggtac ctcgaccgcg
     4741 gggggccgca agagctctac ctcggttcag gggccgtcct caccaccacc ttgccgttcg
     4801 gtctccatca ggtgacgctg gtcgtcggca acgcgtacga cgaaaggtcg acggcgacga
     4861 taccggtcga ggtcgtggat acgcagctgc cgactctggg gctcggtctc acgcccgcga
     4921 ctctctggcc gccgaaccac gacatggtcg agatcgaggc gacgcccgac gcgtcggaca
     4981 actgcgggcc gcccgcggtg cgcctggttt cgatcggcag cagcgagccg gacgacgctc
     5041 ccggcggtgg agacggcacg acgacgaacg acatgcggaa cgccgagatc ggagtcgccg
     5101 acacgggctt cgcgttgcgt gcggagcggg caggtgatgg ggtcgggcgc gtctacgagg
     5161 tgacgtacga ggccgaggat cgcggcgggc tgaaggtgac ggccacgcgc cgcgtgctcg
     5221 tcccccactc tctcggcgga gcgacggagc cgctgttgat ccgccttcac tccgaggcgt
     5281 cggggacgac gatcgaatgg gacgacgtcg gcggcgccgt ctactacaac gtggtgcgcg
     5341 gcgatctggc ggcgccgtcc ctgctcgatt ccgtcgtgca actcggttcg gtcgactgca
     5401 tcgcgagcgg gctcgtcgcc aactcgacgg ccgggtacga ggacgcgggc actccccagc
     5461 cgaacgcggt gttctactac ctcgccgagt accacgacgg ctggcgcgcc agcggctacg
     5521 gctccgaatc ggtcgccggc cctcgctcgc tgtccgtcgg ggactgcgac tgaaggaccc
     5581 gcggcgcggc cggatctcga agtgtcgtgc cgcgtcgtgc tgcgcagtga atcgcgcgcc
     5641 ggcatcgtct tcggggaggc tctcgccggg ttcagcgcgg cagccggaac gagaacccgg
     5701 cgccgacgaa ccagtcctcg gcgcgatcgt tcaggccgac gccgcccgag aaatcgagct
     5761 gcaccgtctc agacgacagg t
//
LOCUS       DASPGN010000245         2308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_159248, whole genome shotgun sequence.
ACCESSION   DASPGN010000245 DASPGN010000000
VERSION     DASPGN010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159248"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VD788_07145"
     CDS             <1..480
                     /locus_tag="VD788_07145"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HXV76083.1"
                     /translation="ATVYDTADSLSHEWWRALVEGFRSHSVLAGRIALISLGVAVIYA
                     AVIFVALAWMRPDHFVTPPGRSAIGPQRHPVLRFSWHAAKNVLGAVLLLTGVAMLVLP
                     GQGMLTILVALTLLEFPGKRRLVLRIVRRPPVHSAIDWLRARAGQPPIVLPSRDDAD"
     gene            877..1257
                     /locus_tag="VD788_07150"
     CDS             877..1257
                     /locus_tag="VD788_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV76084.1"
                     /translation="MDEPRTARTAVVRRAADVPSKTVAAGSATESQLLLGPDDGAPHF
                     AMRRFTMGVGGGMPRHTNAVEHEQYVLEGRAEVEIGGEVHRVASGDVLYIPAGVPHSY
                     RVVEGPFVFLCVVPNQPDRIEIVD"
     gene            complement(1264..2154)
                     /locus_tag="VD788_07155"
     CDS             complement(1264..2154)
                     /locus_tag="VD788_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXV76085.1"
                     /translation="MRILHFSDIHVGGGWARVPRAKWLGKRAVGAANLLAGRGKRFAD
                     GPRKLAALARFREEQRIELVLFTGDYTALGLEPEFASARAAIAPLLAAPGGFVGIPGN
                     HDIYSADVRRERTFLRHFGDTLRTDRPELSVDDGWPLVRLFDGGVAVVALDSTRPNPY
                     PWRSSGRLPPRQLASLARVLDDRTVAGCFVFVLNHYPPRLADGRRDRPFHRLVNDDAL
                     LAACGAIRRGALLSGHVHHRYWLRVPPTGTPLFCSGSATMTGGEGLWLFQTDGSAMQA
                     IPGHFRDGAYRLEPSGAVAL"
BASE COUNT          328 a          792 c          849 g          339 t
ORIGIN      
        1 gcgacggtgt acgacaccgc cgacagcctc tcgcacgagt ggtggcgggc cctggtcgag
       61 ggtttccgct cgcacagcgt gctcgccggg cggatcgcgc tgatctcgct cggggtcgcc
      121 gtgatctacg cggcggtcat cttcgtcgcc ctggcgtgga tgcgcccgga ccacttcgtc
      181 acgccccccg gccgctcggc gatcggcccg caacggcacc cggtgctccg gttcagctgg
      241 cacgcagcga agaacgtgct gggtgccgtg ctgctgttga ccggagtggc gatgctcgtg
      301 cttcccggcc aggggatgct gacgatcctc gtcgcgttga cgctgttgga gttccccggc
      361 aagcgtcggc tggtcctccg catcgtccgc cgcccgcccg tccattcggc gatcgactgg
      421 ttgcgcgcgc gcgccggcca accgccgatc gtgctgccgt cgcgcgacga tgccgactga
      481 ccggggcgcg gtccggaacc ggcagtcgaa ccgatgaccc gcctgcgctc actcgggttc
      541 cgccaccgca gcgagttcag gctcgccggc gtcttttgcg acccgccgcc ggctcgatcg
      601 aggtcgccgt tccgctccac cggctggccg acgcaagccg ccgggggttc cgcatccgcg
      661 gtgtcggcct cgtcctccag gggttcgagc tgctcggcta cctctctccc gtaccgcatc
      721 catccggcgc tcgaacagct cgggatcgag agcgtggtct acgatccctg cgtgctccgg
      781 ccgcggacgg cgaccggctc agcgtgatgc gggcgaacgt cgcccgactg cagcggatcg
      841 cagccggtct ataatgcggc tcgaggaacc caggcgatgg acgagccccg tacggcaaga
      901 accgcggtcg ttcggcgcgc cgccgacgtg ccgtcgaaga cggtcgcggc agggtcggcg
      961 acggaaagcc aactactgct cgggccggac gacggcgcgc cgcacttcgc gatgaggcgc
     1021 ttcacgatgg gggtgggcgg cggaatgccg cgacacacca acgcggtgga gcacgagcag
     1081 tacgtgctcg agggccgcgc cgaggtcgag atcggcggcg aggtgcaccg cgtggcgagc
     1141 ggcgacgtgc tgtacatccc ggccggcgtg ccgcattcct accgggtggt cgagggaccg
     1201 ttcgtctttc tgtgcgtggt accgaaccag cccgaccgga tcgagatcgt cgactgatcg
     1261 cgcttacaag gcaacggcgc cgctcggttc cagccggtag gcgccgtcgc ggaagtgacc
     1321 cggaatcgcc tgcatcgccg atccgtccgt ctggaacagc cacaggccct cgccgccggt
     1381 catcgtcgcg cttccgctgc agaacagcgg ggttccggtt ggcggaaccc gcagccagta
     1441 gcgatgatgg acgtgacccg acagcagcgc gccgcggcgg atggcgccgc aggcggcgag
     1501 cagcgcgtcg tcgttgacca ggcggtggaa cggccgatcg cgccggccgt cggcgagccg
     1561 gggcgggtag tggttgagca cgaagacgaa gcacccggcg acggtccgat cgtcgagcac
     1621 gcgtgcgagc gaagcgagct gccgcggcgg tagtcgtccg ctcgatcgcc aggggtacgg
     1681 gttgggtcgc gtgctgtcga gcgcgacgac ggcgaccccg ccgtcgaaca gccgcaccag
     1741 cggccagccg tcgtcgacgg aaagctctgg ccggtcggtc cgcaacgtgt cgccgaaatg
     1801 gcggaggaac gtccgctcgc gccggacgtc ggcgctgtag atgtcgtggt tgcccggaat
     1861 gccgacgaag ccgccggggg cggcgagcag cggggcgatc gccgctcgcg ccgacgcgaa
     1921 ctccggttcg agtccgagcg cggtgtagtc gccggtgaac agcacgagct cgatccgttg
     1981 ctcctcgcgg aatcgggcga gcgccgccag cttccgcggc ccgtcggcga agcgcttgcc
     2041 gcggccggcc aggaggttcg ccgcgccgac cgctcgtttg ccgagccact tggctcgggg
     2101 cacgcgggcc cagccgccgc cgacgtggat atcgctgaag tggaggattc gcatcggatc
     2161 cagcacaccc ggacgggtgg aagccggaca ggatacaccg tcggggcgcg gccggcgccg
     2221 ggcggacaaa cggcgatcga ggggctaact tcccggtgag ggtcggggcc acgttggttc
     2281 cggtctgcgc cgagcggtag aatcgaag
//
LOCUS       DASPGN010000246         6576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1096707, whole genome shotgun sequence.
ACCESSION   DASPGN010000246 DASPGN010000000
VERSION     DASPGN010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096707"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>6576
                     /locus_tag="VD788_07160"
     CDS             <1..>6576
                     /locus_tag="VD788_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HXV76086.1"
                     /translation="LTLQVNGTDVDVTVLAADTDGTNEQNNSIHDLVADIQSAVNQAL
                     MTAGFQGDEIEIKLYDPNGNRIQFLAVEGEGVNTLAIFIDETNLNNGAFTELGFDPDQ
                     LVDGQGETKKAKADTFFLEDVHFSGEFAVLAHDITATASVGFLGVKATLDPTLELTGD
                     KIIGADVDFALRNPVDGTTRIGVDLIGNAIADGKFFFDAAEGFGGSDEDPQTGFIDGT
                     IDGGVGLSLDISPDGAFSGLPASISASLDLKAESPNWLISPPRLSDPLGFEEVIKSGM
                     AQAGANTNITLAATSASQNDFYNGSKIRITGGTGKVGEVRNITDYDGTTKVATIDAAW
                     TTNPDNSTTYEILLEPIRFDGTNTESLGTALSGGTLAENVSIVVSNDNDGANANPFEA
                     VGLLEAGNYGSAAGLQAALQDVVDDALKRLEDIASLAPGSLAGDITIGLSGSHLTATG
                     EAGMSLRANRIVLDFAGPDFDDILSQFRDFSFDDILAALQLVVDFLQGMKDGGVAGEV
                     FNFQIPLINRSVGDLIDAGDEFLSFVEELSNNPAGSLQELEKFFRDQLDLPQLADPLG
                     LAGGLGSEILKAFNSVSPFTFDQVLSGGKLAEDLSFRLTQGGQGVLVELRAAETALFA
                     SLDDLKDELQDRIDAALAELGVSGTLTVDHVGGQITLTPDGTLVGELLSLKPFSLFSL
                     DIPNGILNLDYNFSAHAEENRPFNLDLSAAGLPAVFTNLIGVSTSGLLAVSADLELNL
                     GLGLDLLGDDKGFYIRTDATNLTASASASGSNLNFSGMIGPLGLFVKGGSASLDAEFS
                     ASMADPNGNGRFNLVALGENLSFDTDLDEIGNFIVVDDLVDDVSDTDTVFLNANASAS
                     LPLFFGLESSPVPLGDPNVLGATIDLVELIVDADPMDSGYSFTLPDVNFNDLTALIPD
                     LFGMLADPAVMIDSLNLVLKTIQDTLNGQIFGFELPLVGDLLADNPVSNFIEDFRVDV
                     LQPFANTLRQSNANLEGLILLIRETFFDAFGPSGLDILKDSNNDDLITLADVGARFLD
                     DTGDPSTDEDGDPVTFLSATALQFDFDIGKEFSFQTPEIVLDLGVPAFGLSANLRPRV
                     NIDFNLHFGFGVHEDLGFYFVADHATPELTADVTVDFGSTTIELPADISEAELEEVLN
                     GLALGGASVVSVVRTTPGSGVEYAITFDQPIHDLLPIEVDGGPNVSITGGGANWTIAL
                     DSATGDFAIAAPAQLVGRLLFLALQVSDGVDLMGDGYDTIFGALTSDAQLPEEDFSKL
                     SLAASLDINEDSGDGRLSFPELFSQSLDDTFDIDVFGAALLRAHAVVDFSTISDEVAR
                     LLPAIHTDIIVDFSISFSDSAGLIVGEPNIGLANIAVDLGDIIDGIAGDILKKVKEIL
                     EPTEWLIGPDGLLNMRIPLLSDLAGSKLTIRNTLLPLFAPDEAPIVNQFLDVVEQLFF
                     LVDLIDDAASDADGLTINFGDFIVTGGEAFKNKYTGVFGGSDVVSLLKGDDDQDITNI
                     SQLQKFDPSKIKIPSAAELETPPGAKSTTQSFVSGVTKEGSFQLELFKPETIFKLLLG
                     QPDVTLFTVELPEFGFEFLYRQSFPIIGPLVGFFSGGIGAHFDFGFGMDTRGLSQFLA
                     SGNPATLINGVFINDLDPATGFDRHEITFTAQIAVGAGVDLGLIKAGVEGGIEATINF
                     NFADLDRDGKVRADELAGNLVANDFNPLSVFDISGLIEFFMRAFVEINLFITQITLEF
                     EFVRLTLFEFNIPFERPAIMASQSGDTLTLNIGSSAAQRLNGDTSDIGETVHVESFGG
                     GVIVWSDQFNVNREVAKISPFFGVTKIVADGGAGNDVIDLELMTDTSIIADIDGGEGD
                     DEITGGWGNDVLVGSGGRDRIFGNKGEDELHGGLGDDELFGDDDNDVLLGEEGHDKLD
                     GGDHDDTLDGGVGNNFYTRSAGADVYTLLNTGSTQFIDGNTGDPELNLTELFGTGITL
                     PTSFDIFLKDGAILVGSGDRLQTLNQILSSTFNFTVGNVPDFYEHQVYVADASGFTDI
                     VGGEFSDNFYVLDTPNAITLNGGAGADHYFLITSDAVGDATATVTIHDVGEGLAEANT
                     IEVVDPELFGFASFADHIRVTETTITLDVGGGSQDTITYDSPAGVINTDQLVLNISTL
                     GLNDVVEIQSTSLTVPVRVDTGAGDDKIVAGGGSEGIDEIKSILQPGGNGNVGFGPLV
                     LV"
BASE COUNT         1248 a         2173 c         2037 g         1118 t
ORIGIN      
        1 cctgacgctg caggtcaacg gcaccgacgt cgacgtcacc gtcctggcgg ctgacacgga
       61 cgggacgaac gagcagaaca actcgattca cgacctggtc gccgacatcc agtcggccgt
      121 gaaccaggcc cttatgacgg cggggttcca gggcgacgag atcgagatca agctgtacga
      181 ccccaacggc aaccgcatcc agttcctggc cgtggagggc gagggcgtca acacgctcgc
      241 gatcttcatc gacgagacca atctcaacaa tggcgcattt accgagcttg gtttcgaccc
      301 ggatcagctc gtcgacgggc agggcgagac caagaaggcg aaggccgaca ccttcttcct
      361 ggaagacgtc cacttcagcg gcgaattcgc tgtccttgcg cacgacatca ccgcgacggc
      421 aagcgtgggc ttcctcggcg tcaaggcgac gctcgacccg acgctcgagc tcaccggcga
      481 caagatcatc ggcgcggacg tggatttcgc gctgcgcaac cccgtggacg gaaccacccg
      541 catcggcgtc gacctgatcg gcaacgcgat cgcggacggc aagttctttt tcgacgctgc
      601 cgagggcttc ggcggcagcg acgaggatcc gcagaccggc ttcatcgacg gcacgatcga
      661 cggcggcgtg ggcctgagcc tcgatatcag ccccgacggc gcgttcagcg ggctgccggc
      721 cagcatctcg gcgtcgcttg acctcaaggc cgagagcccg aactggctca tctcgccgcc
      781 gcggctcagc gatccgctcg gcttcgagga ggtcatcaag agcgggatgg cgcaggccgg
      841 cgccaatacc aacatcacgc tggcggctac gtcggccagt cagaatgatt tctacaacgg
      901 ctcgaagatc cgcattaccg gaggcaccgg caaggtcggc gaggtgcgca atatcaccga
      961 ctacgacggt acgacgaagg tcgcgaccat cgacgccgct tggaccacca acccggacaa
     1021 ctcgaccact tacgagattc ttctcgagcc gatccggttc gacggcacga acaccgaaag
     1081 cctcgggacc gccttgtcgg gcggcacgct ggcggaaaac gtcagcatcg ttgtctcgaa
     1141 cgacaacgac ggcgccaacg caaatccgtt cgaagcggtc ggcctgctcg aggccggcaa
     1201 ctacggcagc gccgcgggcc tgcaagcagc gctgcaggat gtcgtcgacg atgcactgaa
     1261 gcgtctggaa gacatcgcct cgctcgcacc gggaagcctg gccggggata tcaccatcgg
     1321 gctcagcggc agccatctga ccgcgaccgg cgaagccggc atgagcctgc gcgccaaccg
     1381 gatcgtgctc gacttcgcgg gccccgactt cgacgacatc ctgtcgcagt tccgcgattt
     1441 cagcttcgac gacatcctcg cagcgctgca gctggtggtc gacttcctgc agggcatgaa
     1501 ggacggcggc gtcgccggag aggtgttcaa cttccagatc ccgctcatca accgcagcgt
     1561 cggcgacctg atcgacgcgg gcgacgagtt cctcagcttc gtcgaggagc tctcgaacaa
     1621 cccggccggc tccctgcagg agctggaaaa gttcttccgc gaccagctcg acctgccgca
     1681 gctcgccgac ccgctcggcc tggccggcgg cctgggatcg gaaatcctga aggcgttcaa
     1741 ctcggtatcg ccgttcacgt tcgaccaggt attgagtggt ggaaagctgg ccgaggacct
     1801 ttcgttccgc cttacgcaag gcggccaggg agtcctggtc gagctacgcg cagccgagac
     1861 cgcgttgttt gcgtcgctcg atgatctcaa ggatgaactc caggacagga ttgatgcggc
     1921 cctggccgag ctgggcgtaa gcggaacgct cacggtggac cacgtcggag ggcagatcac
     1981 gctcacgccg gacggcacgc tcgtgggtga actgctcagc ctgaagccgt tcagcctgtt
     2041 ctcgctcgac attccgaacg gcatcctgaa tctcgactac aacttctcgg cgcacgccga
     2101 ggaaaaccgt ccgttcaatc tcgacttgtc cgcggcggga ttgcctgccg tgttcacgaa
     2161 cctgatcggc gtgagcacct ccggcctgct cgccgtgtcg gcagatctcg agctgaatct
     2221 cgggctgggg ctcgacctcc tcggcgacga caagggcttc tacatccgca ccgacgccac
     2281 caacctcacg gcctcggcgt cggcgtcggg cagcaacctg aacttcagcg gcatgatcgg
     2341 gccgttgggg ctattcgtga aaggcggaag cgcaagcctc gacgctgagt tttcggccag
     2401 catggccgat ccgaacggca acgggcgctt caacctcgtc gcgctcggcg agaacctgtc
     2461 gttcgacacg gacctcgatg agatcggcaa cttcatcgtc gtagacgatc tcgtcgacga
     2521 cgtgagcgac accgacacgg tgttcctgaa cgccaacgcg agcgcgagcc tgccgctgtt
     2581 cttcggcctg gaaagcagcc cggttccgct cggcgacccg aacgtcctcg gcgcgaccat
     2641 cgacctggtc gaactgatcg tcgacgcaga cccgatggac agcggctaca gcttcacgct
     2701 gcccgacgtg aatttcaacg acctcacggc gctgatcccg gacctgttcg gcatgctggc
     2761 cgaccccgcg gtgatgatcg acagcctgaa cctggtgctg aagacgatcc aggacacgct
     2821 gaacggccag atcttcggct tcgagctgcc gctcgtcggc gacctgctcg ccgacaaccc
     2881 ggtctccaac ttcatcgagg atttcagggt cgacgtcctg cagcctttcg ccaacacgct
     2941 gcggcagtcg aacgcaaacc tggaaggcct gattctcctg atccgggaga cgttcttcga
     3001 tgcattcggt ccctccggcc tggacattct caaggactcg aacaacgatg acctgatcac
     3061 tctcgccgac gtcggcgcca ggttcctgga cgacacgggc gatccatcca cggacgagga
     3121 tggcgatccg gtcactttcc tcagcgcgac cgcgcttcaa ttcgatttcg acatcggcaa
     3181 ggagttttcc ttccagaccc ccgagatcgt gctcgacctc ggcgtgcctg cgttcgggct
     3241 gagcgcgaac ctgcgcccgc gcgtcaacat cgacttcaac ctgcacttcg ggttcggggt
     3301 gcacgaggat ctcggcttct atttcgtcgc cgaccatgcg acgccggagc tcacggcgga
     3361 cgttaccgtg gacttcggct cgaccacgat cgagctgccg gcggacatct ccgaagcgga
     3421 attggaggag gtgctcaacg ggctggcgct cggcggggcc tcggtcgtca gcgtagtcag
     3481 gaccactccg ggatcgggcg tcgagtacgc catcaccttc gatcagccca ttcacgacct
     3541 gctgccgatc gaggtggatg gcggtccgaa cgtgtcgatt acgggcggcg gcgcgaactg
     3601 gaccatcgcg ctcgacagcg ctaccggtga cttcgccatt gccgcccccg cgcagcttgt
     3661 cggccggctg ctgttcctcg ctctgcaggt gtcagacggc gtcgacctca tgggcgacgg
     3721 ctacgacacc atcttcggcg cgctgaccag cgacgctcag ctgcccgagg aggatttcag
     3781 caagctctcg cttgcggcct cgctggacat caacgaggac agcggcgacg gcagactgtc
     3841 gttccccgag ctcttcagcc agtcgctcga cgacaccttc gacatcgacg tcttcggcgc
     3901 ggcgctgctg agggctcatg cggtagtcga cttcagcacg atcagcgacg aggtcgcgcg
     3961 gctgctgccc gcgatccaca ccgacatcat cgtcgacttc agcatctcgt tcagcgactc
     4021 cgccggcctg atcgtcggcg agccgaacat cgggcttgcg aacatcgcgg tcgacctcgg
     4081 cgacatcatc gacggcatcg ccggcgacat cctgaagaag gtcaaggaga tcctcgagcc
     4141 gacggaatgg ctgatcggcc ccgacggcct gctcaacatg cgcattccgc tgctctcgga
     4201 tctcgcgggc agcaagctca ccatccgcaa caccctgctg ccgctcttcg cgcccgacga
     4261 ggcgccgatc gtcaaccagt tcctcgatgt ggtcgagcag ctgttcttcc tcgtcgacct
     4321 gatcgacgac gcggcgagcg acgccgacgg gctgacgatc aacttcggcg acttcatcgt
     4381 caccggcgga gaggcgttca agaataagta cacgggcgtg ttcggcggaa gcgacgtcgt
     4441 cagcctgctc aagggagacg acgaccagga catcaccaat atcagccagc tgcagaagtt
     4501 cgatccgagc aagatcaaga ttccctcggc ggccgagctc gaaacgccgc ccggggcgaa
     4561 gagcacgacg cagagcttcg taagcggcgt cacgaaggaa gggagcttcc agctcgagct
     4621 gttcaagccg gaaacgatct tcaagctgct gctcggccag cccgacgtca cgctgttcac
     4681 ggtggagctg ccggagttcg gcttcgagtt cctttaccgg cagtccttcc cgatcatcgg
     4741 gccgctggtc ggcttcttct cgggcggcat cggcgcgcat ttcgacttcg gcttcggcat
     4801 ggatacccgg ggcctgtcgc agttcctggc gagcggcaac cccgcgacgc tgatcaacgg
     4861 cgtgttcatc aacgacctcg atcccgccac gggattcgac cggcatgaga tcacgttcac
     4921 tgcgcagatc gcggtaggcg ccggcgtgga cctcggtctg atcaaggccg gggtcgaggg
     4981 cggcatcgag gcgaccatca acttcaactt cgcggacctg gaccgcgacg ggaaggtacg
     5041 cgccgacgag ctcgccggca acctggtggc gaacgacttc aacccgctgt cggtgttcga
     5101 catctcgggg ctgatcgagt tcttcatgag ggcgttcgtc gagatcaacc tgttcatcac
     5161 ccagatcacg ctcgaattcg agttcgtacg gctgacgctg ttcgagttca acattccgtt
     5221 cgagcggccc gcgatcatgg cctcgcagag cggcgacacg ctgacgctga acatcggctc
     5281 gagcgccgcg cagcggctga acggcgacac cagcgacatc ggcgagacgg tgcacgtcga
     5341 gagcttcggc ggcggcgtca tcgtctggtc cgaccagttc aacgtcaacc gggaagtcgc
     5401 caagatcagc ccgttcttcg gcgtgaccaa gatcgtcgcc gacggcggcg cgggcaacga
     5461 cgtcatcgac ctcgagctga tgaccgacac gagcatcatc gccgatatcg atggcggcga
     5521 aggcgacgac gagatcaccg gcggctgggg caacgacgtg ctggtgggta gcggcggcag
     5581 ggacaggatt ttcggcaaca agggcgagga cgagctccac ggcggcctgg gcgatgacga
     5641 gcttttcggc gacgatgaca acgacgtcct gctcggcgag gagggacacg acaagctgga
     5701 cggcggcgac cacgacgaca cgctcgacgg cggcgtcggc aacaacttct acacccgcag
     5761 cgccggtgcg gacgtctaca cgctgctgaa tacggggagc acccagttca tcgacggcaa
     5821 taccggcgac ccggagctca acctcaccga gctcttcggc accggaatca cgcttccgac
     5881 ttcgttcgac atcttcctga aggacggcgc gatcctggtc ggctcgggag atcgcctgca
     5941 aacgctcaac cagatcttga gcagcacctt caacttcacc gtcggaaacg ttcccgactt
     6001 ctacgagcac caggtctacg tcgccgatgc ttcgggcttc accgacatcg tcggcgggga
     6061 gttctcggac aacttctatg tgctggatac gccgaacgcc attaccctta atggaggtgc
     6121 aggcgctgac cattacttcc tgatcaccag cgacgccgtc ggcgacgcga cggcgaccgt
     6181 gacgatccac gatgtgggcg agggcctcgc cgaagccaac accatcgagg tcgtcgatcc
     6241 ggaactgttc gggttcgcga gcttcgcgga ccacatccgc gtcaccgaaa ccacgatcac
     6301 gctagacgtg gggggcgggt cgcaggacac gattacctat gactcgccgg ccggggtcat
     6361 caacaccgac cagctcgtcc tcaacatcag cacgctcggg ctgaacgacg tggtggagat
     6421 ccaaagcacc tcgctgacgg tgccggtgcg cgtggatacc ggcgcaggcg acgacaagat
     6481 cgtcgccggc ggcggctcgg agggcatcga cgagatcaag tccatcctgc agccgggggg
     6541 caacggcaac gtgggcttcg gcccgctcgt cctggt
//
LOCUS       DASPGN010000247         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_393927, whole genome shotgun sequence.
ACCESSION   DASPGN010000247 DASPGN010000000
VERSION     DASPGN010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393927"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VD788_07165"
     CDS             complement(<1..1063)
                     /locus_tag="VD788_07165"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV76087.1"
                     /translation="MSLETGGRLLHYRLVEKLGEGGMGVVWKALDTSLDRAVAIKVLP
                     EGLSQDPERLARFEREAKTLAALNHPNIAAIHSVHHQDDRRFIVMEYVVGADLATRLA
                     RGPVPLDETVELMRQVAFALEAAHAGGVVHRDLKPANIVITPDGTAKVLDFGLAKAFY
                     GDLGASAPGNPALSPTVTSAGTLPGVILGTAAYMSPEQARGRAVDRRADVWAFGCVLY
                     EMLSGEVPFPGETVSDVLAGVLKAEPAWDGLPAATPSGVRRVIERCLRKDPRQRYHDV
                     ADLRLDLDERAGRSPHERGPSRAPASRARVAAGVAGCLALGLLAGWLLADRWPDRPVA
                     DRTTRSIVATDPAGRIDAVS"
     gene            1160..>2098
                     /locus_tag="VD788_07170"
     CDS             1160..>2098
                     /locus_tag="VD788_07170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV76088.1"
                     /translation="MTDEPQDRFQLVERIFQRALALPDGDARERYLEQACGGDPELHA
                     RVRSLLDADSRNSGLTDVVHRAAAGLVGTASAGAHDASGRRIGPYRVTGKLGHGGMGV
                     VYRAVRDDDEYNKQVAIKLVSPIGASEDWIRRFRQERNILARLDHPNIARLLDGGTTD
                     DELPYVVMEYVEGRPIDRWCDEQRLDLDERIELFRQVCDAVHEAHRNLVVHRDLKPAN
                     VLVGTDGRPRLLDFGIAKLLDPKVDDAALAAPTRTAGRLLTPEYASPEQVRGLPVSTA
                     SDVYSLGVMLYELLAGVRPYRLDGAPPAEIDRLVCEV"
BASE COUNT          300 a          763 c          758 g          277 t
ORIGIN      
        1 tcgacacggc gtcgatgcgg ccggccgggt cggtcgcgac gatcgaccgg gtggtgcggt
       61 ccgccaccgg ccggtcgggc cagcggtccg ccagcagcca gcccgcgagc agaccgagcg
      121 cgaggcagcc ggccaccccc gcggcgacgc gggcgcgcga ggccggcgcg cgcgatggcc
      181 cccgttcgtg cggtgatcga ccggcacgct cgtcgaggtc gaggcggagg tcggccacgt
      241 cgtggtagcg ctgccgcgga tccttgcgca ggcagcgctc gatcacccgc cgtacgccgg
      301 acggggtcgc tgccggcagg ccgtcccacg ccggctcggc cttcagcacg ccggccagta
      361 cgtccgacac cgtctcgccc gggaacggca cctcgcccga cagcatctcg tacagcacgc
      421 agccgaaggc ccagacgtcg gcgcgccgat cgacggcacg cccgcgcgcc tgctccgggc
      481 tcatgtaggc cgcggtgccg aggatcacgc cgggcagcgt gccggccgag gtcacggtcg
      541 gcgacaacgc cgggttgccc ggtgccgacg ctcccaggtc accgtagaac gccttggcca
      601 gcccgaagtc gagcaccttc gccgtgccgt cgggtgtgat cacgatgttg gccggcttca
      661 ggtcgcgatg gacgacgccg ccggcgtggg ccgcctcgag cgcgaacgcg acctggcgca
      721 tcagctcgac cgtctcgtcg agcgggaccg ggccgcgcgc gagccgggtg gcgagatcgg
      781 cgcccacgac gtactccatc acgatgaagc ggcgatcgtc ttggtggtgc accgagtgga
      841 tcgcggcgat gttcgggtgg ttgagcgcgg ccagcgtttt cgcctcgcgc tcgaaccggg
      901 cgagtcgctc cgggtcctgc gagaggcctt cgggcaggac cttgatcgcg acggcgcggt
      961 cgagcgaggt gtcgagcgcc ttccagacca cgcccatccc gccctcgccg agcttttcga
     1021 cgaggcggta gtgcagcaga cgtccgccgg tctcgagcga cacccgtcat cctccgccgg
     1081 gatcgccgga ttctagcgca gcgggcgttc ccgtagaatg cgggcgggcc ggaggtccgt
     1141 caacggagcg gagggcgtga tgaccgacga accgcaggac cgtttccagc tcgtcgagcg
     1201 gatcttccag cgcgcgctcg ccctgcccga cggcgacgcg cgcgaacgct acctcgagca
     1261 ggcgtgcggc ggcgatcccg agttgcacgc ccgcgtgcga tcgcttctcg acgccgacag
     1321 ccgcaacagc gggctgaccg acgtcgtgca ccgggcggcg gcggggctcg tcgggacggc
     1381 gtccgccggc gcgcacgatg cgtccggtcg ccgcatcggc ccgtaccgcg tgaccggcaa
     1441 gctgggccac ggcgggatgg gcgtcgtcta ccgcgccgtt cgcgacgacg acgagtacaa
     1501 caagcaagtc gcgatcaagc tggtctcgcc gatcggcgcc agcgaggact ggatccgtcg
     1561 cttccgccag gagcgcaaca tcctggcccg gctcgaccac cccaacatcg cccgcctgct
     1621 cgacggcggg accacggacg acgagctgcc ctacgtcgtg atggaatacg tcgaggggcg
     1681 gccgatcgac cgctggtgcg acgagcagcg gctcgacctc gacgaacgca tcgagctgtt
     1741 ccgccaggtc tgcgacgcgg tgcacgaagc ccaccgcaac ctcgtcgtgc accgcgatct
     1801 caagccggcc aacgtgctgg tcggcaccga cgggcggccg cgcctgctcg acttcgggat
     1861 cgccaagctg ctcgacccga aggtggacga tgcggcgctc gccgcgccga cgaggaccgc
     1921 cggccggctg ctgacgccgg agtacgccag cccggagcag gttcgcggcc tccccgtgtc
     1981 gaccgcgagc gacgtctatt cgctcggcgt gatgctgtac gagctgctgg ccggagtccg
     2041 gccgtaccgc ctcgacgggg caccaccggc cgagatcgat cgcctggtgt gcgaggtc
//
LOCUS       DASPGN010000248         3948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_394262, whole genome shotgun sequence.
ACCESSION   DASPGN010000248 DASPGN010000000
VERSION     DASPGN010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394262"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /locus_tag="VD788_07175"
     CDS             complement(<1..1337)
                     /locus_tag="VD788_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76089.1"
                     /translation="MNLSGSWSRVGFVLAFVAVASAAASAERGLTFTQLAAIREVGEI
                     RVSPDGSRIAYTLEIPRTIGVDDDGPEWVELWLTGIGGGDPRPFVHGDVKVETVRFSP
                     DGSLITYLAKRADDEHGALYAIPVDGGESRRLVDFDETIRDYRLSPDGRRVAFVAREP
                     ESEAAKQAKEHGYKQEVFEEDWRQQKLWIAELAPFTPTVGDPDAPNQDDDRKPRALPI
                     DGAVFGVDWTPDGKQLLLEVAPTPLIDDRFMRRRIGVYDVDSGERSASIANVGKLGAV
                     ELSPDGRNVAMIAAADANDPLEGRLAVAPIGGGEPRDLLPGLLGHVTALAWRDPATVA
                     YLADVGEETIYGEVGLTGGEGRELLRSGSARADGAPVPVMTRVALSADGAVTALVGES
                     PRHPPELFVLAGEESVARRLTDSNPWLGEVELADQELYRHEARDGLSLSGVLIR"
     gene            1632..2912
                     /locus_tag="VD788_07180"
     CDS             1632..2912
                     /locus_tag="VD788_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV76090.1"
                     /translation="MGASEEQGRSHRRRPTAGRVRVWPAVAIAVTLGGGLVWIWAFYG
                     EQRQSRVLATIAACGLAAVGWLVWLVAFSGLRRRTRLLAVGGIVLLVAVLALSLERRG
                     LTGDALPLFSWRFATSGAGPAVPEARPAAARSADAESDYPQFLGPRRDATLPGRRFAT
                     DWRSHPPRELWRRPIGAGWSGFAIAGPYAVTQEQRGDSELVTGYDLRTGRLLWVHRDR
                     TRHDDPLGGPGPRATPSIADGRVFAVGGTGRLQVLELHTGTSLWGLDLLRETGAAPPQ
                     YGVAGRRFRIAGDRVEGMHAELSWESSALEAKFTNVVLHQGHLYGLDDGILVAVDVAD
                     GRRVWKKGRYGHGQLVLVGDVLLIQAESGDVVIVRATPTGHEELGRLAAIDGRTWNHP
                     AVAGRLLLVRNDREAACFELVSANEERENDGGHG"
     gene            2905..3768
                     /locus_tag="VD788_07185"
     CDS             2905..3768
                     /locus_tag="VD788_07185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV76091.1"
                     /translation="MADNGTNKKRRRGPGERTVPHRHLLYSAAVQSPETDLDFFERVY
                     RRARGTTFRVLREDFCGTAAMACEWVKRRADNRAIGIDLDPATLAWGKRHYVPVLGRA
                     AERLELIQGDVRDVTRPPADVIAAQNFSYSVFKTRAELGGYFRRVRRSLNDGGLFFVD
                     AFGGTDTLGADVESRRVESTLSFDGRKVPSFTYVWEQASFNPVDHHIVCYIHFKLRGG
                     ERRNKAFRYDWRLWTLPELRELMLDAGFATAEVWIEGWDDEADDTDGVFRRRKRFENM
                     SGWIAYVVGSS"
BASE COUNT          566 a         1373 c         1406 g          603 t
ORIGIN      
        1 cggatcagca cgccgctcag cgacagtccg tcgcgcgcct cgtggcgata gagctcttgg
       61 tcggcgagct cgacctcgcc gagccagggg ttgctgtcgg tcagacggcg cgctaccgac
      121 tcttcgccgg ccagcacgaa cagctccggc gggtgccgcg ggctctcgcc gaccaacgcc
      181 gtcaccgcgc cgtccgccga cagggcgacg cgcgtcatca ccggcaccgg cgcgccatcg
      241 gcgcgcgcgg agcccgagcg cagcagctcg cgcccttcgc cgccggtcag accgacctcg
      301 ccgtagatcg tctcctcgcc gacgtcggcc aggtaggcca cggtcgccgg gtcgcgccag
      361 gccagtgccg tgacgtgccc gagaagcccc gggagcagat cccgcggttc accgccgccg
      421 atcggcgcga ccgccagccg gccctcgaga ggatcgttgg cgtcggccgc cgcgatcatc
      481 gccacgtttc gtccgtcggg gctgagctcc accgcgccga gtttcccgac gttggcgatc
      541 gacgccgatc gctcgccgct gtcgacgtcg tagacaccga tccgcctgcg catgaaacgg
      601 tcgtcgatca acggcgtcgg cgcgacctcg agcagcagtt gcttgccgtc gggcgtccag
      661 tcgacgccga acacggcgcc gtcgatcggg agcgcgcgcg gctttcggtc gtcgtcctgg
      721 ttcggtgcgt cggggtcgcc gaccgtcgga gtgaacggcg cgagctcggc gatccacagc
      781 ttctgctgtc gccagtcctc ctcgaacacc tcctgcttgt agccgtgctc cttcgcctgc
      841 ttcgcggctt cgctctccgg ctcgcgtgcg acgaaggcta cgcgccggcc gtcgggcgac
      901 agccggtagt cgcgaatcgt ctcgtcgaag tcgaccaagc gccgtgactc gccgccgtcg
      961 accggaatcg cgtagagtgc gccgtgctcg tcgtcagcgc gcttggcgag gtaggtgatg
     1021 aggctgccgt ccggcgagaa gcggacggtc tcgaccttca cgtcgccgtg gacgaacggc
     1081 cgcgggtcgc cgccgccgat cccggtcagc caaagctcga cccactccgg tccgtcgtcg
     1141 tcgacgccga tcgtccgggg gatttcgagc gtgtacgcga tgcggctgcc gtccggggag
     1201 acccgaatct cgccgacctc gcgaatcgcg gccagttgcg tgaaggtcag accgcgctcg
     1261 gcggaggccg ccgcgctcgc gaccgcgacg aacgccaaga cgaaaccgac gcggctccaa
     1321 ctcccagaca ggttcattcc gactcctcgc gatcgccagc cgatcgccac tcgagcggtg
     1381 cgcgcgcgat cgcgcgatcc ggggcgagtc cggcgagcgc cactacggga accagcgcac
     1441 ggggaatggt cgctgattcg gtcgcggcgc ggcgccgggc gcgcgaaagc ggaatcgtac
     1501 atcgaagtcc cgctgcgcgg actcgtcgcc gcgcgaaaca gcggtccgcc ccacccggtg
     1561 acggcccggc tacctgcgcg atctcgtctc agggttggca gaaaccgacg atcgtgctct
     1621 tatctcgccg agtgggcgca tccgaagaac aaggccgctc ccaccggcgg cggccgaccg
     1681 cgggcagggt tcgggtttgg ccggccgtcg cgatcgccgt caccctcggc ggtggcctgg
     1741 tctggatctg ggcgttctac ggcgagcagc gtcaatcgcg cgtactggcg acgatcgcag
     1801 cctgtgggct ggccgcggtc ggttggctcg tctggctggt cgcgttctcc ggtttgcggc
     1861 gacgcacgcg cctgctcgcg gtcggcggca tcgtcctgct cgtggccgtg ctcgcgctgt
     1921 cgctcgaacg gcgcggcctc acgggtgacg cgctgccgct gttctcgtgg cgcttcgcca
     1981 cgagcggagc cgggccggcc gttcccgagg cccgaccggc cgcagcgcgc tcggcggacg
     2041 ccgaatccga ctacccgcag ttcctcggtc cgcgacgcga cgcgacgctt ccgggccgcc
     2101 gattcgcgac ggactggcgg tcccaccccc cgcgcgagct gtggcgccgc ccgatcgggg
     2161 ccggttggtc cggcttcgcc atcgcgggac cctacgcggt cacccaggag cagcgcggcg
     2221 acagcgagct cgtcaccggc tacgacctgc ggacgggaag gctgctgtgg gtccatcgag
     2281 accgcacgcg gcacgacgat ccgctgggcg gcccggggcc gcgtgccaca ccatcgatcg
     2341 ccgacgggcg ggtgttcgcc gtcggcggca ccgggcggct ccaggtgctc gagctgcaca
     2401 ccggcacgtc gctgtggggc ctcgacctgt tgcgcgagac cggcgcggcg ccaccgcagt
     2461 acggcgtcgc cggcaggcgg tttcgcatcg cgggcgatcg cgttgaagga atgcatgccg
     2521 aactgtcgtg ggagtcgagc gcgctcgagg ccaagttcac caacgtcgtg ctccaccagg
     2581 gccacctcta cggtctcgac gacgggatcc tcgtcgcggt cgacgtcgcc gacggacgcc
     2641 gcgtgtggaa gaaggggcgc tacgggcatg gccagctggt cttggtcggc gacgtgctgc
     2701 tgatccaagc cgaatcgggc gacgtcgtga ttgtgcgggc gaccccgacc ggtcacgagg
     2761 agttgggtcg actggcggcg atcgacggga ggacgtggaa ccacccggcg gtcgcgggac
     2821 gcctgttgct ggtcaggaac gaccgcgagg cggcgtgttt cgagctcgtc tccgccaacg
     2881 aggaacggga gaacgacggc gggcatggct gacaacggga cgaacaagaa acgacgacgg
     2941 gggccgggcg agcggacggt cccgcaccgg catctgctct attcggcggc cgtccagtcg
     3001 cccgagacgg atctcgactt cttcgagcgg gtctacagga gggcccgcgg tacgaccttc
     3061 cgcgtcctgc gcgaggactt ttgcggaacc gctgcgatgg cgtgcgagtg ggtcaagcga
     3121 cgggccgaca accgtgcgat cggcatcgac ctcgacccgg cgacgttggc gtggggcaag
     3181 cgacattacg tcccggtgct gggtcgagcg gccgagcggc tcgagctgat ccagggcgac
     3241 gtgcgcgacg tgacgcggcc gccggccgac gtgatcgcgg cgcagaactt ctcgtactcg
     3301 gtgttcaaga cacgggccga gttgggcggc tactttcggc gcgttcgccg atcgttgaac
     3361 gacggaggcc tgttcttcgt cgacgccttc ggcggcaccg acacgctcgg cgccgacgtc
     3421 gagagccgcc gggtcgagtc gacgttgtcg ttcgacggca ggaaggtgcc ttcgttcacc
     3481 tacgtttggg aacaggcgag cttcaacccg gtcgatcacc acatcgtgtg ctacatccac
     3541 ttcaagctcc gcggcgggga acgccggaac aaggcgttcc gctacgactg gcggctgtgg
     3601 acgctgcccg agctgcggga gctgatgctc gacgccggct tcgcgacggc cgaagtctgg
     3661 atcgaaggtt gggacgacga ggccgacgac accgacggcg tgttccggcg gcggaaacgg
     3721 ttcgagaaca tgagcggttg gatcgcctac gtcgtcggat cgagctgacc cgccactgcc
     3781 cgaggacaaa aacgcggccg cgccgtggcg cggcctctcc gattcgagcc gactcgcctc
     3841 gatccggcga tcagtcaccc aggctgatca ccgtatcggt ccacagggcg gtgtcgctcc
     3901 acagggcggt gtcgctccac agggcggtgt cgctccacag ggcggtgt
//
LOCUS       DASPGN010000249        13262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1565150, whole genome shotgun sequence.
ACCESSION   DASPGN010000249 DASPGN010000000
VERSION     DASPGN010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 13262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13262
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565150"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1430
                     /locus_tag="VD788_07190"
     CDS             234..1430
                     /locus_tag="VD788_07190"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXV76092.1"
                     /translation="MSPLLAAKSSSSPELAAALVAASLRPELRGDGTPYLELLERGDG
                     LAELLDAAAQHGVLGLTLSTLLDALRRGIDRPPAALEVRFEDLRRRSSMLELKCALVV
                     SLLERARVNPVVLKGAAIALRHYPEPWQRDLADLDLLVRPHDVDAALATLAAAGYRSP
                     VSPARDAYYRAKHFHLPVVHGDGHYVEIHWALTRPDSGFGLDPESVYWSAERVTRQGG
                     AIIRIPRVEHTMLHIVVQNLQEGFSRLGRTVDIDRIVASTPRLDWDQLVAEARRGNLG
                     SATALSLQLAARLMHTPVPDEVRRALLPPPVARFHLALMRPELHLLSRRVTRIRAAED
                     LLELWLLNVARRRFGLLASNLRRFGAIRGAIRVAKISALQLLFYAGALLPGTSRSARA
                     FWSFAD"
     gene            1487..1762
                     /locus_tag="VD788_07195"
     CDS             1487..1762
                     /locus_tag="VD788_07195"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HXV76093.1"
                     /translation="MQARDRPRKTDRFMFKDLGDEFLLYDPQGEQIHVLNGTAREVLL
                     LCDGRRSIVELVAALVERFEVDEATAERDVGELLEHLSELGAIETEG"
     gene            complement(1770..2405)
                     /locus_tag="VD788_07200"
     CDS             complement(1770..2405)
                     /locus_tag="VD788_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV76094.1"
                     /translation="MPDGNHEEGRRFFTTSEVARYCAVTNDGVLKWIKSGKLRAFSTP
                     GGHYRVSAEDFRAFLEQFDIPIDESFFRGSSRQRSVLIVDDEPDIREVVRRWLKELDE
                     ELRVEEACDGYEAGIKIGSMQPDLVILDVMMPRVDGISLCRSIKDNPKTEAVKVLAIT
                     AFPEQDNVRKMYDAGADLCLIKPLQMEHFRLEVIRLMNEAAQHTPGRASSA"
     gene            2610..2686
                     /locus_tag="VD788_07205"
     tRNA            2610..2686
                     /locus_tag="VD788_07205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2644..2646,aa:Arg,seq:acg)
     gene            2721..3200
                     /gene="tadA"
                     /locus_tag="VD788_07210"
     CDS             2721..3200
                     /gene="tadA"
                     /locus_tag="VD788_07210"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF008113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HXV76095.1"
                     /translation="MNRELDDDRFMRLALELAQQAGVRDEVPVGALVVAGGRVVGRGA
                     NAPIGRRDPTAHAEILALREAAEKAGNYRLVGATIYCTVEPCLMCLGAAVHARIDRLV
                     YGADDPKVGSIRALDRLASDGAALNHRFAVTGGVLADESAELIRRFFRDRREQPVGG"
     gene            3217..3309
                     /locus_tag="VD788_07215"
     tRNA            3217..3309
                     /locus_tag="VD788_07215"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3252..3254,aa:Ser,seq:cga)
     gene            3327..3416
                     /locus_tag="VD788_07220"
     tRNA            3327..3416
                     /locus_tag="VD788_07220"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3361..3363,aa:Ser,seq:gga)
     gene            3441..3540
                     /gene="ffs"
                     /locus_tag="VD788_07225"
     ncRNA           3441..3540
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VD788_07225"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            3570..5261
                     /gene="dnaX"
                     /locus_tag="VD788_07230"
     CDS             3570..5261
                     /gene="dnaX"
                     /locus_tag="VD788_07230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXV76096.1"
                     /translation="MYQVLARKCRPQVFEELVGQGHVTRTLANAIAGERLAHAYIFAG
                     LRGSGKTTVARILAKCLNCETGPTATPCGRCVPCREIAESRAIDVLELDAATRTQVDN
                     IRELQEVIAYAPARDRYKVLIIDEAHMLSKNAANALLKTLEEPPPRVVFVLATTEIQK
                     ILPTIVSRCQVFEFRRVAHGELTDYLRWICAGERIEIDDRALERIARAGEGSVRDSLS
                     VLERALAFSGGTIGDDDVMRMLGGVRFEVVRDLVAGLAARDTSAMLDILDALVDEGHD
                     LLHFWSEVIGALRDLLLLQAAPSRLAALDRPADEAKRLTEAAAGLSTEDLTRAFQILA
                     DLEPVLKTSSQPRFMFEAALIRLASLGAVRPIEQVLAEIRTGTATAEPTAAASRPAAA
                     RPPAQKKNEAESVTARPGEDRTGRFVAAVFGARPMLGSILERAQAIRVIGDALAIRLA
                     AGAGSLARQLERKENLEVLVEHSSRLFGRTLRIVIESDDEAATVDSARAASAGSAAAV
                     ERPPTGRRSKGGGKRGSLVEVARSEPGVSKLLRDFGAQVVDIRPLPPSDGTDRES"
     gene            5289..5588
                     /locus_tag="VD788_07235"
     CDS             5289..5588
                     /locus_tag="VD788_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002107277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXV76097.1"
                     /translation="MNIGKMMKDLQKMQAKMQSEMEELEVEASSGGGMVTARMNGKKE
                     LLSIKIASDAITPDDPELLEDLVTAAVNEAGRKVDRSLQDLTRGLAGGMNIPGLT"
     gene            5614..6213
                     /gene="recR"
                     /locus_tag="VD788_07240"
     CDS             5614..6213
                     /gene="recR"
                     /locus_tag="VD788_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXV76098.1"
                     /translation="MSSLAPPLERLIEQLCKLPSVGRKTAQRLAFHLLKAPVDETRAL
                     ADAIRELRERIRLCADCFNLTEDRRCPICLDERRDRTRICVVEEPGNLLALERTGVFR
                     GLYHVLGGALSPLKDVGPGELRIRELVSRLEAGGFAEVILATNPDVEGEATAVYLSRL
                     IAPLGIPVTRLAQGLPAGGDLEFTDDLTLARALEGRRSY"
     gene            6303..6791
                     /locus_tag="VD788_07245"
     CDS             6303..6791
                     /locus_tag="VD788_07245"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HXV76099.1"
                     /translation="MSRACLVVHEQDHASFMAVLERLRTESNSRFIFLLDKNGQQIAK
                     TGDLGGSDPTALASLAAGNVAATEGLAQLIGESEFSTLFHEGEKDSLHISVIANKVIL
                     LVIFDEHSSLGLVRLRVKQRSDELAGILEAMLRRGDGEAAGIPASTLGEITDEDIDAL
                     FG"
     gene            6809..7393
                     /locus_tag="VD788_07250"
     CDS             6809..7393
                     /locus_tag="VD788_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HXV76100.1"
                     /translation="MTFINYAAREINCKIVYYGPGLGGKTTNIQWIHQKTRNESKGKL
                     ISLATETDRTLFFDFLPIELGTIRGFRTRFHLYTVPGQVFYDASRKLILKGVDGVVFV
                     ADSQKMRRDANAEAIDNLRTNLRDNGYELNQLPYVLQFNKRDLPTAMAAEEMARELRL
                     RDEPVFEAVATRGDGVFDTLKAVIKLVLADLRKR"
     gene            7476..7790
                     /gene="rplU"
                     /locus_tag="VD788_07255"
     CDS             7476..7790
                     /gene="rplU"
                     /locus_tag="VD788_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXV76101.1"
                     /translation="MYAVIESGGRQFRVQVGDTVRVERLDGDPGAPVVFDRVLMVGAG
                     DDTTVGGPTVDGAAVRGSILGHGREKKVRIYTFKRRQNSNRRRKGHRQDYTAVKIEAI
                     ES"
     gene            7812..8069
                     /gene="rpmA"
                     /locus_tag="VD788_07260"
     CDS             7812..8069
                     /gene="rpmA"
                     /locus_tag="VD788_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXV76102.1"
                     /translation="MAHKKAGGSSRNGRDSNSKRLGVKRFGGELVTGGSIIVRQRGTP
                     IKAGLNIGRGKDDTLFALTDGRVVFEDRGRMGRFVRIEPVA"
     gene            8098..9786
                     /gene="obgE"
                     /locus_tag="VD788_07265"
     CDS             8098..9786
                     /gene="obgE"
                     /locus_tag="VD788_07265"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXV76103.1"
                     /translation="MFVDEATIQAIGGDGGNGCLSFRREKYVPRGGPNGGDGGDGGSV
                     ILRADGGTSTLLAFRFQRLYRADRGRHGEGSNKKGRSGEDRVVRVPVGTVVLDEDGLA
                     VLADLDRDGAVFVAARGGRGGRGNARFATSTHQAPTRHDPGEPGESRQLRLELKLLAD
                     VGLVGLPNAGKSTLISRISAARPKIADYPFTTLDPHLGVVDGGDGRSFVVADIPGLIE
                     GAHLGAGLGQRFLRHVERCSLLVQLIDPLDPETDPVRAAEVVEREIASYRAALGDRPR
                     WLVATKADTLSDRARLDGLRAHAADAGRPLFVISAVSGEGVGDLVRAMAEAVSHRERR
                     VESSHFEVVAVLGGTFDPVHRGHLELAEEVRRRTGLDRLLLVPGAIPPHKRHAPVTPA
                     WHREAMLRLAVRGRPGIELCTAELESGGVSYTVETLRELRDHGTGSTPVFVLGMDSLE
                     ELPTWRDYRGLIREFDLVVVDRPGAGPPDRCVDPEIAARLVEVGEASGRGWFARQDLG
                     AGGRIFHVRLPPMPVSSSEVRARIARGEEIGDLVPPDVARYILEHGLYAEEGIR"
     gene            9807..10169
                     /gene="rsfS"
                     /locus_tag="VD788_07270"
     CDS             9807..10169
                     /gene="rsfS"
                     /locus_tag="VD788_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXV76104.1"
                     /translation="MVHCVEAAHDKKAEDLVVLDLRNISDVTDYFVICHGTSDRQVLT
                     IADSIEQRLAERLRVKPGSIEGRRRGEWVLMDYIDFVVHVFVAEKREFYRLERLWGDA
                     PRADLEVLAAGKRQRSAR"
     gene            10190..10654
                     /locus_tag="VD788_07275"
     CDS             10190..10654
                     /locus_tag="VD788_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXV76105.1"
                     /translation="MKIRLLVVGKPRDAGAAALVERYLERIRRFGVSVDTVRVPEVRA
                     GGRFTDEHVLEREAAKLLARLEPPGTVVAIDRGGRELSSAELPGKLERWATPRATFVV
                     GGPLGLHETVRRRADFAWSLSRLTLPHELALVLVAEQLYRGMTLLRGVPYHK"
     gene            10710..12080
                     /locus_tag="VD788_07280"
     CDS             10710..12080
                     /locus_tag="VD788_07280"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76106.1"
                     /translation="MRVRTFVTILLLLVVLWGIAAMWTANRDVLAHTVYLWGETTTTV
                     GWLLIAFLALGVLGTILLGGVRELSLMIERSRLRKVGRKYEEIEDEYSRGLVADLEGR
                     EEEALAHFRKTLEWDSKHFNTLIKIGEVLRSQERVAEAIEYHRKAHHLKEDSPRPLYA
                     LVEDYEARGDMDQARAVLGQIIAIDKNSVAAWRKLRSIHVKERHWEPALEAHDRVVRL
                     HAPNDPQAPADRRTGRGIRYEIASERLARGKVREAIGLLRRLIKDDPTFIPAHVKLGH
                     ALQKQGHETEATEAWFSGFEATGSPIFLTVLEEHFLKREEPFAAIEALKRCIGASRKD
                     TLARFYLGKLYFRLEMLDDAFTVLSALRGRASYAPTLHYLLGRINERRNHHSAANEEY
                     RHVIKEMDLVQLEYLCQACDATLIEWSDRCPRCGEWQSVEVNFREEISLDELGLSAAP
                     IYPSRP"
     gene            12117..12884
                     /locus_tag="VD788_07285"
     CDS             12117..12884
                     /locus_tag="VD788_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HXV76107.1"
                     /translation="MRQHTLRRAGRHAGLALLDFLLPTRCFVCDALLPPLALRGACAS
                     CWAALCPVAPDACPGCALAGPSDLLGPAGDRCADCRMAPPPFDTVRAAVVYDDAARAI
                     VLRAKDGRSRELYRELARMMEATLRVAAPELAGIPIVPVPSHPWNDLVRGYSPAGEIA
                     RRLRASSGCPRFALLSRRWRSPRNTKRLSGVSRRALADRGIRLRAPLAVRRLVLVDDV
                     MTTGATLGACARVLRAAGAEEIHALVWARTPRKAAGS"
BASE COUNT         2066 a         4441 c         4652 g         2103 t
ORIGIN      
        1 cgagaagctc aagtacgacc tgtactacat caagaaccac aacgtcctgc tcgacctgtg
       61 gatcatcctc aagacggtgc gcgtcgtcct gtcggcgcac ggcgcccgct gacccgtcac
      121 tttacttcgc ccccgccaga atgtagattc cggagcggat tcgatctgca tcggcgagcc
      181 gtgggcgaag ttcgccagcg ccgggtgctt gaccgcggat cgtccgaggc gcgatgtccc
      241 ccctactcgc tgccaagagc agttcgtctc cggagctcgc ggccgcgctc gtcgccgcgt
      301 ccctccgccc ggagttgcgc ggcgacggca ccccctacct cgagctgctc gagcgggggg
      361 acggtctcgc cgagctgctc gacgccgcgg cgcagcacgg ggtgctcggc ctgacgctct
      421 ccacgctgct cgacgcactc cgccgcggga tcgaccgtcc gccggccgcg ctcgaagtcc
      481 ggttcgagga cctcagacgc cggtcgtcga tgctcgagct gaaatgcgcg ctcgtcgtct
      541 cgctgctcga gcgggcgcgc gtcaatccgg tcgtgctgaa gggcgcggcg atcgcgctcc
      601 ggcactaccc ggagccgtgg cagcgcgatc tggccgacct cgacctgctc gtccgcccgc
      661 acgacgtcga tgccgcgctc gcgacgctgg cggcggccgg ctaccgttcg ccggtgagcc
      721 cggcgcggga cgcgtactac cgggccaagc actttcacct tccggtggtc cacggcgacg
      781 ggcactacgt cgagatccac tgggcgctga cgcggccgga ctcgggcttc ggtctcgacc
      841 cggaaagcgt ctactggtcg gccgagcgcg tgacgcgcca gggcggggcg atcatccgca
      901 ttccgcgtgt cgagcacacg atgctccaca tcgtggtgca gaacctccag gagggcttct
      961 ccaggctcgg ccgcacggtc gacatcgacc ggatcgtcgc gtcgacgccg cgcctcgact
     1021 gggaccagct cgtcgccgag gcgcgtcgcg gcaatctggg gtcggccacg gcgttgtcgt
     1081 tgcagctcgc cgcgaggctg atgcacacgc ccgtaccgga cgaagtccga cgggcgctgc
     1141 tgccgccccc ggtcgcgcgg ttccacctcg cgctgatgcg ccccgagctt cacctgctgt
     1201 cgcgccgggt gacgcggatc cgcgcggccg aggacctgct cgagctgtgg ctgttgaacg
     1261 tggcgcggcg ccgcttcggt ctgctcgcgt cgaacctccg tcggttcggc gcaatccgcg
     1321 gtgcgatccg cgtggccaag atcagcgctt tgcagttgtt gttctacgcc ggcgcgctgc
     1381 ttcccgggac gagccgcagc gcgcgcgcgt tctggagctt cgccgactga gacgactcca
     1441 ctccacgtta caatacccgc ggatgcgagc caaggagcga cggcgcatgc aggcacgaga
     1501 ccgaccgcgc aagaccgatc gtttcatgtt caaggatctc ggcgacgaat tcctgctcta
     1561 tgacccgcaa ggcgagcaga tccacgtgct caacgggacc gcgcgcgagg tgttgctgct
     1621 gtgcgacggg cgccggtcga tcgtcgagct cgtcgccgcg ctggtcgagc ggttcgaggt
     1681 cgatgaggcg acggcggaac gcgacgtggg cgagctgctc gagcacctca gcgagctcgg
     1741 ggcgatcgag accgaaggct gaccgccgct caggcgctcg acgcccgtcc cggggtgtgc
     1801 tgggcggcct cgttcatcag acggatcacc tccagccgga agtgctccat ctgcagcggc
     1861 ttgatcaggc agaggtcggc cccggcgtcg tacattttcc gcacgttgtc ctgctcggga
     1921 aacgcggtga tggcgagcac cttgaccgct tcggtcttcg ggttgtcctt gatcgaccgg
     1981 cagagcgaga tgccgtcgac tcgcggcatc atcacgtcca ggatgacgag atccggctgc
     2041 atgctgccga tcttgatccc ggcttcgtag ccgtcacagg cctcctcgac gcgaagctcc
     2101 tcgtccagct ccttgagcca gcggcgcacg acctcgcgga tgtccggctc gtcgtcgacg
     2161 atcaagaccg agcgctggcg gctcgacccg cggaagaacg actcgtcgat cgggatgtcg
     2221 aattgctcga gaaacgcccg gaaatcttcg gcgctgacgc ggtaatgccc gccgggcgtc
     2281 gagaacgccc gcagcttgcc cgatttgatc cacttgagca cgccgtcgtt ggtcaccgcg
     2341 cagtatcgag cgacctcgga cgtggtgaag aatcgacgac cctcttcgtg gttcccatcc
     2401 ggcattgcgc gactcccgac cgcccggtct ttccggcttt tctgctttgt cggggtgaga
     2461 atctaggttc ccgattgcag tgaggcaacc cgccaggaaa cgcggggcaa ccggcccacg
     2521 gcggtttcgt tgacaccccc ataccccggt gataaactcg cgcattacgc tgaattgggc
     2581 tagtttcctg ggttttttcg ggttttcgac ggaccgtagc tcagctggat agagcgactg
     2641 gctacgaacc agtaggtcgg gggttcgagt ccctccggtc cgaccacgga agccgtcgaa
     2701 ttcctggcgg ggtggtccgg atgaaccgcg agctcgacga cgatcggttc atgcggcttg
     2761 ccttggagct tgcccagcag gcgggcgtcc gcgatgaggt cccggtcggc gcgctggtcg
     2821 tcgccggcgg ccgcgtggtg ggtcgcgggg cgaacgctcc gatcggccgc cgcgacccga
     2881 cggcccacgc ggagatcctg gccctgcggg aggccgccga gaaggccggc aactaccgct
     2941 tggtcggggc gacgatctac tgcaccgtcg aaccgtgctt gatgtgtctc ggcgctgcgg
     3001 tccacgcgcg gatcgaccgg ctggtctacg gcgccgacga cccgaaggtc ggttcgatcc
     3061 gtgcactcga ccggctggcg agcgacggcg cggcgctcaa ccatcggttc gccgtgaccg
     3121 gcggcgtgct cgccgacgag tcggcggagt tgatcaggcg tttcttccgc gatcggcgtg
     3181 agcagccggt cggcggatga cggaatccag gtaaacggag aggtaccgaa gtggccgtaa
     3241 cgggggcgcc tcgaaagcgc tttgtctgcg tgagcaggca cgtgggttcg aatcccaccc
     3301 tctccgccac tcgagatcga gagccgggag agatgaccga gtggcttaag gtgcacgctt
     3361 ggaaagcgtg tgtgcgggta accgtaccgt gggttcgaat cccactctct ccgccaagcg
     3421 actcgtagta accttgtggg gcccaagccc tgtgcactgt gacctcgtga accccgccag
     3481 gcccggaagg gagcaacggt aagcgaatcg tcgcaggtgc cgcaggtcaa cttgggcccc
     3541 ttcctcatca gggcgacggg agctcggacg tgtatcaggt gctggcgcgg aaatgtcgcc
     3601 cgcaggtctt cgaggagttg gtcggccagg gccacgtcac gcgcacgctg gccaacgcga
     3661 tcgccggcga gcggctggcg catgcctaca tcttcgccgg cctgcggggt tccggaaaga
     3721 cgaccgtcgc ccgcatcctg gccaagtgtc tcaactgcga gacggggccg accgcgacgc
     3781 cgtgcggtcg ctgcgtgccg tgccgcgaga tcgccgagag ccgcgcgatc gacgtcctcg
     3841 agctggacgc ggcgacccgc acccaggtcg acaacatccg cgagctgcaa gaggtgatcg
     3901 cctacgcccc ggcccgcgac cggtacaagg tgctgatcat cgacgaagcg cacatgctgt
     3961 cgaagaacgc ggcgaacgcg ttgttgaaga cgctcgagga accgccgccg cgcgtggtgt
     4021 tcgtgctggc gacgaccgag atccagaaga tcctgccgac gatcgtctcg cgctgccagg
     4081 tgttcgagtt ccgccgggtg gcccacggcg agttgaccga ctacctgcgc tggatctgtg
     4141 ccggcgagcg gatcgagatc gacgatcgcg cgctcgaacg catcgcgcgc gccggcgagg
     4201 gctcggtgcg cgattcgctg tccgtcctcg aacgggcgct cgctttttcc ggcggcacga
     4261 tcggcgacga cgacgtgatg cggatgctcg gcggcgtccg cttcgaggtc gtgcgcgatc
     4321 tggtcgccgg gctcgccgcg cgcgacacaa gcgcgatgct cgacatcctc gacgccctgg
     4381 tcgacgaggg gcacgacctg ctgcacttct ggagcgaggt gatcggtgcg ctgcgcgacc
     4441 tgctgctgct gcaggccgca ccgtctcgcc tggcggcgct cgaccgaccg gccgacgagg
     4501 cgaagcggct gaccgaggcc gccgccgggt tgagcaccga ggacctgacg cgagcgttcc
     4561 agatcctcgc cgatctcgag ccggtgctga agacgtcgag ccagccgcgc ttcatgttcg
     4621 aggcggcgct gatccggctg gcgagcctcg gcgcggtacg tccgatcgag caggtgctgg
     4681 ccgagatccg caccggcacg gcgaccgccg agccgaccgc cgccgcctcg cgtccggcgg
     4741 cggcgcgacc gcccgctcaa aaaaaaaacg aagctgagtc ggtgaccgcg cggccgggcg
     4801 aagaccgcac cggtcgtttc gtggcggcag tcttcggcgc gcgcccgatg ttggggtcga
     4861 tcctcgagcg ggcgcaggcg atccgcgtca tcggcgacgc gctggcgatt cgcctcgccg
     4921 cgggtgccgg ctcgctcgcc cggcagctcg agcgcaagga gaacctcgaa gtgctggtcg
     4981 agcattcctc gcgtctgttc ggacggacgc tgaggatcgt gatcgagtcg gacgacgagg
     5041 cggcgacggt cgactccgct cgggcagcga gcgccggctc ggcggcggcg gtcgagcggc
     5101 cgccgaccgg gcgccggtcg aaaggcggcg ggaaacgcgg tagtctggtc gaggtggcca
     5161 ggagcgagcc gggcgtgtcg aagctgttgc gcgatttcgg ggctcaggtc gtcgatatcc
     5221 ggccgctgcc gccgagcgac ggcacggatc gcgaatcttg aggcgactcg acggacggga
     5281 ggagcggggt gaacatcggc aaaatgatga aggacctgca gaagatgcag gccaagatgc
     5341 agagcgagat ggaagagctc gaagtcgagg ccagctccgg tggcgggatg gtcaccgccc
     5401 ggatgaacgg caagaaggag ctgctgtcga tcaagatcgc ctccgacgcg atcacgccgg
     5461 acgaccccga gctgctcgag gacctggtga ccgccgcggt caacgaggcg ggtcgcaagg
     5521 tcgatcgatc gctgcaggac ctgacccgcg ggctggccgg cggcatgaac atcccggggc
     5581 tgacctagct accccgacga cccggacgat ccggtgagca gcctcgcccc gccgctcgaa
     5641 cgactgatcg agcaactgtg caagctgccg agcgtcggac gcaagacggc gcagcggctg
     5701 gcgttccact tgctgaaggc gccggtcgac gagacgcgag cgctggccga cgcgatccgc
     5761 gagctacgcg agcggatccg tctgtgcgcc gactgcttca acctgaccga ggaccgacgc
     5821 tgcccgatct gcctcgacga gcgacgcgac cggacacgga tctgcgtcgt cgaggagccg
     5881 gggaacctgc tcgcgctcga acggaccggc gtgtttcgcg gtctgtacca cgtcctcggc
     5941 ggcgcgctgt cgccgctcaa ggacgtcggt cccggcgagc tgcgcatccg cgagctggtc
     6001 tcgcggctcg aagccggcgg cttcgccgag gtgatcctcg ccaccaaccc cgacgtcgaa
     6061 ggcgaggcga ccgcggtcta tctctcgcgc ctgatcgcgc cgctcggcat cccggtgacg
     6121 cggctggccc agggcctgcc cgccggcggg gacctggagt tcaccgacga cctgacgctg
     6181 gctcgggcgc tcgaggggcg ccgcagctac tgagcgcgac cgcgggcttg cgtgtcgcgc
     6241 gaccgcggct atagatttag cggcgtttgt ccccgggcag cggtctgagt cgcggaggcg
     6301 ggatgtctcg agcctgtctg gtagtgcacg agcaggacca cgccagcttc atggcggtcc
     6361 tcgagcgcct gcgaacggaa tcgaactcgc ggttcatctt cctcctggac aagaacggcc
     6421 agcagatcgc caagaccggc gacctcggcg gaagtgaccc gaccgccctc gcctcgctcg
     6481 cggcgggcaa cgtcgcggct accgaaggtc tggctcagct gatcggcgag tcggaattct
     6541 cgacgctgtt ccacgagggc gagaaggaca gtctccacat cagcgtgatc gcgaacaagg
     6601 tcatcctgct cgtgatcttc gacgagcact cgtctctcgg attggtccgg ttgcgcgtga
     6661 agcagcgcag cgacgagctc gccgggatcc tcgaggcgat gctgcggcgc ggcgacgggg
     6721 aggcggcggg aatcccggcc tcgactctgg gcgagatcac cgacgaagac atcgacgcgc
     6781 tgttcggctg atccacggag cgcgagacgt gaccttcatc aactacgccg cccgcgagat
     6841 caactgcaag atcgtctatt acggccccgg cctcggcggc aagacgacga acatccagtg
     6901 gatccaccag aagacccgca acgagtcgaa gggcaagctg atctcgctcg cgaccgagac
     6961 ggaccggacg ctgttcttcg atttcctgcc gatcgagctc ggcacgatcc gcgggttccg
     7021 cacccggttc catctctaca cggttcccgg tcaggtgttc tacgacgcat cccgcaaact
     7081 gattctcaag ggtgtggacg gcgtcgtctt cgtcgccgat tcccaaaaga tgcgacgcga
     7141 cgccaacgcc gaggcgatcg acaacctgag gaccaacctg cgcgacaacg gctacgagct
     7201 caaccagctc ccttacgtgc tgcagttcaa caagcgcgac ctgccgacgg cgatggcggc
     7261 ggaggaaatg gcgcgcgagc tgcgcctgcg cgacgagccg gtgttcgagg ccgtcgcgac
     7321 ccgcggcgat ggggttttcg acacgctcaa ggcggtgatc aagctcgttc tggccgacct
     7381 gcgcaagcgc tagacccgcc ttgacaggcc gatgaccggt gtctacactc ccggttttgc
     7441 cgggcagttc cacggcgctc accgggagca tccagatgta tgcggtgatc gaatcgggcg
     7501 gacgtcagtt ccgcgtgcag gtcggcgaca cggtcagggt cgaacggctc gacggggatc
     7561 cgggcgcacc ggtcgtcttc gaccgcgtgc tgatggtcgg agcgggagac gacacgaccg
     7621 tcggtggccc gaccgtcgac ggtgccgccg tccgcggctc gatcctcggt cacggacgcg
     7681 agaagaaggt gcggatctac accttcaagc gccgccagaa ctcgaaccgc cgtcgcaagg
     7741 gtcaccgcca ggactacacg gcggtcaaga tcgaggcgat cgagtcctga agcggacctg
     7801 ggagtatcgc gatggcccac aagaaagcag gcggaagctc acggaacgga cgcgactcga
     7861 actcgaagcg cctcggcgtc aagcgcttcg gcggcgagct ggtcacgggt ggttcgatca
     7921 tcgtgcgcca gcgcgggacg ccgatcaagg ccggcctcaa catcggtcgc gggaaggacg
     7981 acacgctctt cgcgctgacc gacggtcggg tcgttttcga ggaccgcggc cgcatggggc
     8041 gcttcgtccg catcgagccg gtcgcctgat cttcctcgcg ctccggcgcc tgacaacatg
     8101 ttcgtcgacg aggcgacgat ccaagcgatc ggtggcgacg gcgggaacgg ctgcctgtcg
     8161 tttcgacggg agaagtacgt cccgcgcggc ggcccgaacg gcggcgacgg gggtgacggc
     8221 ggctcggtga tcctgcgcgc cgacggcggc acctcgacgc tgctcgcctt ccgattccag
     8281 cggctgtatc gagccgatcg cggccggcac ggcgagggca gcaacaagaa gggacgttcg
     8341 ggcgaggatc gggtcgtgcg ggttccggtc ggcacggtcg tcctcgacga ggacggcttg
     8401 gccgtgctcg cggatctcga ccgcgacggc gccgtcttcg tcgcggcccg gggcgggcgc
     8461 ggcgggcgcg gcaacgcgcg tttcgccacg tcgacccatc aggcaccgac gcgacatgac
     8521 ccgggcgagc ccggcgagag ccgccagctg cgactcgagc tgaagctgct ggccgacgtc
     8581 ggcctggtcg ggctgcccaa cgccggcaaa tcgacgctga tctcgcggat ctcggcggcg
     8641 cgccccaaga tcgcggacta cccgttcacc acgctcgacc cgcatctcgg cgtggtcgac
     8701 ggcggcgacg gccgctcctt cgtcgtcgcc gacatccccg gcctgatcga gggggcccat
     8761 ctcggcgccg gcttgggtca gcgcttcctg cggcacgtcg agcgctgctc gctgttggtc
     8821 cagctgatcg acccgctcga ccccgagacc gatccggtgc gggccgccga ggtcgtcgag
     8881 cgggagatcg cgagctaccg tgccgctctc ggcgaccggc cgcgatggct cgtcgcgacc
     8941 aaggccgaca cgctttcgga tcgggcgcgg ctcgacgggc tgcgggccca cgcggccgac
     9001 gccgggcggc cgctgttcgt catctcggcg gtcagtggcg agggggtcgg cgacctggtc
     9061 cgggcgatgg ccgaggccgt gtcgcatcgc gagcgacggg tcgaatcgag ccatttcgag
     9121 gtcgtcgccg tgctgggcgg caccttcgac ccggtgcatc ggggtcacct cgagctggcc
     9181 gaggaggtgc gccggcgaac cggcctcgat cggctgttgt tggtcccggg cgcgatcccg
     9241 ccccacaagc ggcatgcgcc ggtcacgccg gcgtggcacc gcgaggcgat gctgcggctg
     9301 gccgtccggg gtcggcccgg gatcgagctg tgcacggcgg agctcgaatc gggtggtgtc
     9361 agctatacgg tcgaaacgct gcgggagctg cgggaccacg gcaccggttc gactccggtc
     9421 ttcgtgctgg ggatggactc gctcgaggag ctgcccacct ggagggacta ccgcggtctg
     9481 atccgcgagt tcgacctggt cgtcgtcgac cggccgggcg ccggccctcc cgaccggtgc
     9541 gtcgacccgg agatcgccgc gcgtctggtc gaggtcggcg aggcgtcggg ccggggctgg
     9601 ttcgcgcggc aagacctcgg cgccggcggg cggatttttc acgttcggct gccgccgatg
     9661 ccggtctcct cgagcgaggt ccgggccagg atcgcgcgcg gcgaggagat cggcgatctt
     9721 gtccccccgg acgtcgcccg gtatattctc gaacacgggc tctacgccga ggagggaatc
     9781 cgctgagcaa caagctaccc gacgaaatcg tccactgcgt cgaagcggcc catgacaaga
     9841 aagccgaaga cctcgtcgtc ctcgatctcc gaaacatcag cgacgtcacc gattatttcg
     9901 tgatctgcca cggaacgtcc gaccggcagg tgctgacgat cgccgacagc atcgaacagc
     9961 ggctcgccga gcggcttcgg gtgaagccgg gtagcatcga aggccggcgc cggggcgagt
    10021 gggtgctgat ggactacatc gacttcgtcg tccacgtctt cgtcgcggag aagcgggagt
    10081 tctaccgtct cgaacggctg tggggcgacg caccgcgagc cgacctcgaa gtgctggcgg
    10141 cgggaaagcg ccaacgtagc gcgcgctgat cccgcccgag cgggccccga tgaagatccg
    10201 tctgctcgtc gtcggaaagc cgcgcgacgc cggggcggcg gcgctcgtcg aacgttacct
    10261 cgagcggatc cgtcggttcg gggtgtcggt cgacaccgtg cgggtcccgg aggtgcgcgc
    10321 cggcgggcgc ttcaccgacg agcacgtgct cgagcgggag gccgcgaagc tgctcgctcg
    10381 gctggagccc cccgggacgg tcgtcgcgat cgatcgcggc ggccgggagc tgtcgagcgc
    10441 agagctgccg gggaagctcg agcgctgggc gacgccgcgg gcgaccttcg tcgtcggcgg
    10501 gccgctcggt ctccacgaga cggtccggcg ccgcgccgat ttcgcctggt cgctctcccg
    10561 gctgaccctg ccccacgagc tcgcgctcgt gctggtcgcc gagcagctct atcggggaat
    10621 gacgctgcta aggggggtgc cgtaccacaa gtgagtcccg cgcctgtggc ttgacgccct
    10681 cgtcgccgct gaactagtct ggatcgccca tgcgtgtacg cacgttcgtc acgatcctgc
    10741 tgctgctggt cgtcctgtgg ggcatcgcgg cgatgtggac ggcgaaccgc gacgtgctcg
    10801 cccacaccgt ctacctgtgg ggcgagacga cgacgaccgt cggctggctg ttgatcgcgt
    10861 tcctcgcgct cggcgtgctc gggacgattc tgctcggggg ggtccgcgag ctgagcctga
    10921 tgatcgagcg gtcgcggctg cgcaaggtcg ggcgcaagta cgaggagatc gaggacgagt
    10981 actcgcgcgg gctggtggcc gacctcgagg gccgagagga agaggcgctc gcccatttcc
    11041 gcaaaacgct cgagtgggac agcaagcact tcaacacctt gatcaagatc ggtgaagtgc
    11101 tgcgcagcca ggagcgggtg gccgaggcga tcgagtacca ccggaaggcg caccatctca
    11161 aggaagacag cccgagaccg ctgtacgccc tcgtcgagga ttacgaggca cggggcgaca
    11221 tggaccaggc gcgcgccgtc ctcggtcaga tcatcgcgat cgacaagaac tcggtcgcgg
    11281 cctggcgcaa gctgcgctcg atccacgtca aggagcgcca ctgggagccg gcgctcgagg
    11341 cgcacgaccg cgtcgtcagg ctccatgcgc cgaacgaccc gcaggcaccg gcggaccggc
    11401 ggaccggccg gggcatccgc tacgagatcg catccgagcg actggcgcgc ggcaaggtcc
    11461 gggaggcgat cggcctgctc cgccgcctga tcaaggacga cccgacgttc atcccggcgc
    11521 acgtcaagct cggccacgcg ctacagaagc aggggcacga gaccgaggcg accgaggcct
    11581 ggttctccgg tttcgaggcg accgggtcgc cgatcttcct gacggtgctc gaggagcact
    11641 tcctgaagcg ggaggagccg tttgccgcga tcgaggcgct caaacgctgc atcggcgcat
    11701 cgcgcaaaga cacgctggcg cgcttctacc tcggcaagct gtactttcgc ctggagatgc
    11761 tcgacgacgc gttcacggtg ctctccgcgc tgaggggccg ggccagctat gcgccgaccc
    11821 tgcactatct cctcgggcgg atcaacgagc ggcgcaacca ccacagcgcc gcgaatgaag
    11881 aataccgcca cgtcatcaag gagatggacc tcgttcagct cgagtacctg tgccaggctt
    11941 gcgacgcgac gctgatcgaa tggtcggacc gctgtccgcg ttgcggcgag tggcagtcgg
    12001 tcgaggtcaa cttccgcgag gagatctcgc tcgacgagct ggggctgtcc gcggccccga
    12061 tctacccgag ccgcccctga cccgaccggt cccgccgcct cccggcgacc cgcccgatga
    12121 gacaacacac gctacgccgt gccggccgtc acgccggtct cgcgctgctc gatttcctgc
    12181 tgccgacgcg ctgcttcgtc tgtgacgcgt tgctcccgcc gctcgctctc cgcggggctt
    12241 gtgcgagctg ctgggcagcg ctctgcccgg tcgcgcccga cgcctgcccc gggtgcgcgc
    12301 tcgccggccc ttccgatctg ctcgggcccg ccggagaccg ctgcgccgac tgccggatgg
    12361 cgccgcctcc gttcgacacg gtccgcgcgg cggtggtcta cgacgacgcg gcgcgggcga
    12421 tcgtcctgcg cgcgaaggac gggcgctcgc gcgagctcta tcgcgagttg gcgcggatga
    12481 tggaagccac gcttcgcgtc gcggcaccgg agctcgccgg catcccgatc gtcccggtcc
    12541 cgtcgcaccc gtggaacgat ctggtgcggg gctacagccc cgccggcgag atcgcgcgcc
    12601 gcctgcgggc ttcgagcgga tgccctcggt tcgccctgct ctcccggcgc tggcgctcgc
    12661 cgcggaacac gaagcgtctg tcgggagtct cgcgccgggc gctcgccgac cggggcatcc
    12721 gcctccgcgc tccgctcgcc gtgcggcgac tcgtgctggt cgacgacgtg atgacgaccg
    12781 gcgcgacgct cggcgcctgc gcgcgggtgc tgagagccgc cggcgccgag gagattcacg
    12841 cgctggtgtg ggcgcgtacc ccgcgaaaag cggcgggttc gtgacaaccc ctcgggtcgg
    12901 gtatttttct cctgcgcgtt cggcggtttc agtgtagatt cagggtcttg gaacccgcga
    12961 gtggttggtc cgaagctggg ttcggttcgt ggcgcgaagc ttcgagattg ttaggtaatt
    13021 gtggtattag cggattccga ggggtcgggg gcaaccagtg gaagaccagc tacgaaagcg
    13081 ccaaaatttt cttgacgaag cgccgaatag acgtatatct acggcacttc agagatgcca
    13141 aggtcatcca gtcctgaaac ctggagcggg gaggtcatgt taatgagaaa ggtaaaacgc
    13201 ctagccgcgg tggcagttgc gctgatcctc acggcagcgg tcgctgctcc ggcgatcgcc
    13261 gg
//
LOCUS       DASPGN010000250        15485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1565369, whole genome shotgun sequence.
ACCESSION   DASPGN010000250 DASPGN010000000
VERSION     DASPGN010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 15485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15485
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1565369"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /gene="arsB"
                     /locus_tag="VD788_07290"
     CDS             complement(<1..899)
                     /gene="arsB"
                     /locus_tag="VD788_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HXV76108.1"
                     /translation="MARLSFLDRYLTLWIFVAMATGVAAGHFVPSLAASIDRLRVGTT
                     HVPIAIGLILMMYPPLAKVRYEELGDVFRDTRVLALSLFQNWVVGPALMFVLAVVFLR
                     GYPEYMTGLILIGLARCIAMVIVWNDLAGGDTEYAAGLVAFNSIFQLLFFSLYAWLFV
                     TFLPPLFGLDGSLVPVGMRQIAESVSIYLGIPFLAGMITRFVLVRRYGKDWYHRVFIP
                     RISPITLVALLFTILVMFGFKGDSIVRIPLDVLRIALPLTVYFVVMFLVSFWLGRAIG
                     ADYRKTTTLSFTAASNNFELAIAV"
     gene            complement(902..1267)
                     /locus_tag="VD788_07295"
     CDS             complement(902..1267)
                     /locus_tag="VD788_07295"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXV76109.1"
                     /translation="MREFLRLVKALGDESRLRALLTLREGELCLCQIIDVLGLAPATV
                     SRHMNVLYQAGLVERRKQGKWQYYRLAAAGGGSPAALRALAWLLDELKQDPALRDDAR
                     RVRRVRRQDLVDLAACYRS"
     gene            1365..2762
                     /locus_tag="VD788_07300"
     CDS             1365..2762
                     /locus_tag="VD788_07300"
                     /inference="COORDINATES: protein motif:HMM:NF015563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HXV76110.1"
                     /translation="MRKTRFPDSLVLIFGIIVCAQLLSYVVPHGVYDRVPVEGSSRTM
                     VADGTYHRVGGDERVRLAPWAFLTAIPEGLAAAQEIIFLVFLVGGVIALLRESGAIDA
                     VLHGAVKRLGRAPWILIAGTLVVFGLGSFTIGMGEEYVPLIPILVTMCLAMKLDSIVA
                     MGMIWVPYAIGWACAGTNPFGVVIAQGIAGVPITSGLATRSVLLVLFLAIGFQHVYAY
                     ARRIQRDPGASLVSDIDYSTGFDMPHDVRLDWRRIVILTTLVGGIVGFVYGVKTWGWY
                     IVEMNAIFLGIGLAAAVLSRMTPNAAAQTFIRGAAEMTAAALLIGFARTIEVVLSQGQ
                     IIDTIIHSIAKSLEGSGSYVATLGMLGVQTICNFFIPSGSGQAYVTMPIMSPLATLTG
                     VPQQVAVLAYQFGDGFTNMVVPTSALVMGTLALGKIPYTRWVRFVTPLLIKLFVVACI
                     VLLAAVRFGESWGFY"
     gene            complement(2781..3974)
                     /locus_tag="VD788_07305"
     CDS             complement(2781..3974)
                     /locus_tag="VD788_07305"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006169.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXV76111.1"
                     /translation="MADVHAFRERFELRNEAAAGGPLGGLRFAAKDVFDVAGRRVGGG
                     SPDWLASHGPAARTAPVVQACLDAGAVLVGYTIADEMSFSPFGENEHYGTPVNPAAPD
                     RVPGGSSSGSASATAAGEVDFALGADTAGSVRIPASFCGLFGIRPTHGRLSSAGVMPL
                     APSFDTPGWFAREAGLLERVGRVLLPGGGDETSSIERLLLLEETLEIAHSATRHAFEA
                     ALERVTSRIGPVRPAGLSRLRYRDWLEAYDTLRPPEIWRAHGEWIERTRPRFGSKTAA
                     RFETVRARAGADTTAAAAFRAKMQRVAGELLGDDGAFVLPTTPSIAPLKGISDRDAPE
                     LRQHIFQLSGIAPMLGFPEITLPLMQVDGYPVGLSLIGPPGGDRALLAAAVEICGAAG
                     QPSVS"
     gene            complement(3977..4540)
                     /locus_tag="VD788_07310"
     CDS             complement(3977..4540)
                     /locus_tag="VD788_07310"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV76112.1"
                     /translation="MSRDDPQTVTMLLRRWRDGDSGALDRLMPLVYDELHRLAVGLMR
                     SERRDHTLQATALIHEAYARLAGAELPVHDRAHFVSLSARVMRRVLVDYARARRREKR
                     GSGGERVTLAEAMAVVPDAADKLIEIDELLDRLRASDERKSRTLELSLFGGLTQPEIA
                     EALGVSLATVERDLRFARAWLRAELDR"
     gene            4618..5139
                     /locus_tag="VD788_07315"
     CDS             4618..5139
                     /locus_tag="VD788_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76113.1"
                     /translation="MRSARLLPLVLLCLTGPLAAADEGEVYVYTNADLERLGPLPAGE
                     AEPPTADQLAESWAFVQSTIDDAHARIDADRGYELDRRLTEAEADALDRAGGSPRYTL
                     PYNYALGYPYGFGYHDRGRGDRLRRAASRLWERPNAELFEPIVPIHARPYQTNLLRAK
                     TSGRRGFDRPTRH"
     gene            5196..8000
                     /locus_tag="VD788_07320"
     CDS             5196..8000
                     /locus_tag="VD788_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV76114.1"
                     /translation="MVDEDGDRLDPGQRIDRYEIVRVEGSGGMGVVYRARDTVLDRDV
                     ALKRPAGELWQSPESLRRFAREARAAARLSHPHIVQVHEVLEHDGVPWLSLQFVPGKS
                     LAARLAAGERPSIDRVIEWAEQLAAALRHAHERKVLHRDVKPANVLVTDDGRALLTDF
                     GLARSLPRGEADSVERPAGSPGVTLDDRIAGTPSYMSPEQMLGRELDERSDLFSLGLV
                     LYEICSGRPLFAPGTAQEAREALLVHSPPSVRAANRAVPRALDRVIGKLLAWRPEGRF
                     ADAGLLVAELRRCREERARRPRRVWLAAAGGAALLLALAVLGSLRFAGGPAGERRAPD
                     VVRAMALVTWPGGEWEARVSPDGHFISFLSNHDGGQRLWLRDVTGGQVEPLTAGEAWV
                     ASHVWSAEGDEIAYLVRAPGGPRLEIVAPGGGPVRRAAALDDRLDGARLLRFLGDRLY
                     LETAANELVGFDLARGTHATLIDGRAEGRHRAGFDVRPDGRRAAWAERGASGCAIWTG
                     ALDGGDAQAVTSGDHCDVDPLWVGAGEGLVYTSSRSGQLDLWRVEPAGGRPERITLSA
                     GSEVATGSSADGSLVVFVQRSEIANLWMYVPAKRLHLQLSADALGDALPASVPTSSII
                     AFERTRPRLDESTGLFGAQILIGRLAGERMVESKQVAAEGEQALLAPDGQWVAYARGD
                     ELWVQDVATGHAVRVSTRYRRPAVGRFPLARSRENAVWLERGPALCFVEYAGGGTRVA
                     RYPAGGAAGEVRPVAETRDGVAIDDLWASPGGRRLAWIEREHGTSRLRLHDTDGGASR
                     TLVDEDGPLWLDGWIDDRSLLVVRDLPAAGDVAPSIALSRVALDGSIASLGEVERAFV
                     DTVRLVPAARRIYLTAAGDDRVDNLIAVSLDDGSRETLTDNTVTDVTFSTVAPAPDGA
                     LIYTRQQRVADLWGIEFRPQ"
     gene            8083..10935
                     /locus_tag="VD788_07325"
     CDS             8083..10935
                     /locus_tag="VD788_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV76115.1"
                     /translation="MMDDGSDIGIDVPLPSDGPPEPRYRPGDRVARYEIRGVVGAGGM
                     GVVYRALDTRLDREVALKCPWGTLADNSAALKRFEREGRAAARLFHPNIVEIHEVFQH
                     DGLPWLALQFIEGESLRAKLERTGALPLEDVLSWAEQLAAALEHAHAQNILHRDLKPA
                     NVLVTPDGRAMLTDFGLARPIKTRPDAGGEASGTVSGTITLDGHLVGTPAYMSPEQAL
                     GRPLDGRSDIFSLGALLYEMCCGRPLIVKGSAGDARSALVGFEPPSIRELNPRVPHGF
                     ERIVRKALAWRPEDRYPDAAALKSDLADYRRAAARWPRWVPPAAAAAVVALLLAAVLL
                     TRHYSRAEPTDVGPGEVAAEALVTWPSRESDPKLSPDGRWVSFLSDRGGVPQVWLRSL
                     TGTLVEELTAAAAAVTAHAWSPDGREIVRLVNHPDGPLLQRIPALGGLPRDSVTLDER
                     FAGARLVRWIGHRVYVELGERSLVRVDLESGETTPLMTDSAAAGLRWGFDVLPDESRV
                     VYSGGRDFRYAIWTSDLDGSNVEQWTTGEYCDTSPRFLDAEGRYVVFTSTRSGQNDLW
                     RVALDDRALVPITLSTSTEVVGDVSRSGHLIVVEENRDEAHLWALDPRSADARQLTSD
                     ALSDSWPSLGPGSPFLAFLRRLPRLDTTTGVFESRVLRGRLDDTGLVEHEEIAETGAQ
                     PLLSPDGQWVAYLVGRELRVRHAPTGRVVHASDGFVAPASDLYLYANVTTNAVWHPHD
                     PRLYFVDRDDSGFVVKQIDLARSGPAEALGSYGRDTRVDDLSVAPDGGHLAYVSESRD
                     VDGFEVEAIELATGEVRGLLHVDDWRPYTKGWLDDHTLLVAGTPEGRGRGTIFLHALG
                     LNGHLRELGRFGPGLVNTLTFDPRARTVYATVEDEARLQNLVAFSLDGGGPRRLTDNR
                     LPDAGFSTAQLAPDGSIVFVRAKHTQDIWSIEFRRR"
     gene            complement(10946..11482)
                     /locus_tag="VD788_07330"
     CDS             complement(10946..11482)
                     /locus_tag="VD788_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76116.1"
                     /translation="MKHAGRATKVALGSLLLSLIVTAVVLSDPAEPVRHEFSAGELAK
                     IRLRITEIESLAASAYMSDSNTYDAIIAALTQALNDLDGEPGKHTEPGKHDEPPDTGK
                     AEWWEIETIKLFVTNLELVTTKEYTEDPASYETILPLLDRVIDQLKVLIKCSCSPAQP
                     YCCCMKCQARPCDWGQCK"
     gene            complement(11597..14299)
                     /locus_tag="VD788_07335"
     CDS             complement(11597..14299)
                     /locus_tag="VD788_07335"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV76117.1"
                     /translation="MRIEPGTQLMHYRIVEKLGEGGMGVVWKAVDESLGREIAIKVLP
                     ETFAGSADRLARFEQEARLLASLNHPNIAAVYSVHAHEGLRFLAMELVDGEDLATRLA
                     RGPLALDETLDIAIQIARALEAAHDHGVIHRDLKPANVRLTSAGRVKVLDFGLAKGVG
                     PETTSGNPALSPTITSAGTVAGVILGTAGYMSPEQARGRAVDRRADVWAFGCVLYEML
                     SGRRAFDGETVSDTLASILKVDPDWDRLPADTPPSIRRLIRRCLTRDPDRRLAHLAGA
                     LLPMSETRDGVDPERAGEAAPRPARRATWARVLPWTLVVLLGVAAVGSWRGSRVPPAP
                     APLLSLVAPFPDALSIDDQQMGVIALSPDGRKLALSLNDGAVPRLLLRDLREGGEPVV
                     VGGTESAVTPFFSPDGQWVGFFANNKVWKVPVDGGNPIAICEASGNNRGASWGADDRI
                     VFSAHYTMPLSVVGAGGGRPEPLTEIRADRGERTHRWPQYLPELDLVLFTVGTLDSPE
                     NYDGARIEALRPSTGERRTVLEGASIARWIPGGYLLFSRDGFLFATRFDVERLATRGT
                     PVPVVHDVMGMRSSGIVHFDFAADGLLAYVAGESVSRESRMVWLEPDGRIEPIDAPVA
                     GYTDPRISPDGRSIAVAIEGGSTFDIWIYDIERETSTRLTFEGDNTFPTWSPDGTRIL
                     FASVRNNAAVGTYAKNADGSGTDELVYAPDVAAHQGKAVPTDWAPDGRTVLIDFTDER
                     AGNLMAVDLVDGERRVLLATPAGEHTGRISPDGRFLAYTSDESGRAEVYVRPHPGPGG
                     KWQVSTDGGLHPTWSRDGKRLYFRQADEILVADVEAGDGRLRAGRPRKIVGGFPSRLL
                     NVDYDVAPDGRVLIIRSERDDDRPTGVTFLVNWRQELERRLSAE"
     gene            complement(14324..15277)
                     /locus_tag="VD788_07340"
     CDS             complement(14324..15277)
                     /locus_tag="VD788_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXV76118.1"
                     /translation="MLRIPITLAAAVFAAGTLFAAGPARRVAVTIDDLPVVSPTLTDN
                     AAWRRITTDLLAALDEHEVPAVGFVNEKKLHAGDEVDPERVALLVAWLDAGFELGNHT
                     FSHPDLHRVELETFLDDVVRGERVTRPLLAGRERRLVWFRHPYLHTGRDRETREAVHA
                     LLDRHGYRVAPVTIDNSEWIFARAYDLAQSRDDETSMKRIADGYVGYMDRKFAYFERQ
                     TDELFGRAIPHVLLIHANQLNADHFGRLAVAIRARGYVFVGLDEVLEDEAYRSPDDYF
                     GAGGISWLHRWALTRGKSGEFFRGEPLTPEFVKQAAGVDSE"
BASE COUNT         2127 a         5523 c         5639 g         2196 t
ORIGIN      
        1 acggcgatcg ccagctcgaa gttgttgctc gccgcggtga atgacagcgt cgtcgtcttg
       61 cggtagtcgg cgccgatcgc gcggccgagc cagaagctga cgaggaacat cacgacgaag
      121 tagacggtga gcgggagggc gatacgcagc acgtcgagcg ggatgcgcac gatgctgtcg
      181 cccttgaagc cgaacatcac gaggatggtg aacagcagcg cgacgagcgt gatcgggctg
      241 atccgcggga tgaacacccg gtggtaccag tccttgccgt agcgccgcac cagcacgaag
      301 cgcgtgatca tcccggcgag gaacgggatg ccgaggtaga tcgacacgct ctcggcgatc
      361 tgccgcatgc cgaccggcac caggctgccg tcgagaccga acagcggcgg taggaaggtg
      421 acgaacagcc aggcgtagag gctgaagaac agcagctgga agatgctgtt gaacgcgacg
      481 agacccgcgg cgtactcggt gtcgccgccc gccaggtcgt tccagacgat gaccatcgcg
      541 atgcagcgtg ccaggccgat caggatcagc ccggtcatgt actccgggta cccgcggagg
      601 aacacgacgg cgagcacgaa catcagcgcc gggccgacga cccagttctg gaacagcgac
      661 agcgccagca cgcgcgtgtc gcggaagacg tcgcccagct cttcgtagcg caccttggcc
      721 aacggcgggt acatcatcag gatcagaccg atcgcgatcg gcacgtgggt cgtgccgacg
      781 cgcagacggt cgatcgacgc ggcgagcgac gggacgaagt ggccggcggc gacgccggtc
      841 gccatcgcga cgaagatcca cagcgtcagg tagcggtcga ggaaggaaag acgtgccatc
      901 gtcagctccg gtagcaggcg gcgaggtcga ccaggtcctg gcggcgcacc cgtcgcacgc
      961 gccgggcgtc gtcgcggagc gccgggtcct gcttcagctc gtcgagcagc caagccagcg
     1021 cgcgcagcgc ggcgggcgag ccgccgccgg ccgccgccag ccggtagtac tgccacttgc
     1081 cctgtttgcg gcgctcgacc agcccggcct ggtacagcac gttcatgtgg cgcgacacgg
     1141 tggccggggc cagaccgagg acgtcgatga tctggcacag gcacagctcg ccctcgcgca
     1201 gggtcaacag cgcccgcagc cggctctcgt cgccgagcgc cttgaccagc cggaggaact
     1261 cgcgcatcgc cactccgtta cttcgttgac tgacgaagta tagccgatcc gcgccgcggg
     1321 cgacaggggc aggcccggcg gcggtttgcc gctagaatgc gccgatgcga aagacgcgct
     1381 ttccggattc gctggtgctg atcttcggca tcatcgtctg tgcgcagctc ctgagctacg
     1441 tcgtgccgca cggcgtctac gaccgcgtcc cggtcgaggg gtcgagccgc acgatggtcg
     1501 ccgacgggac ctaccaccgc gtcggcggcg acgagcgcgt ccggctggcg ccgtgggcgt
     1561 tcctgacggc gatccccgaa gggctggccg cggcgcagga gatcatcttc ctcgtcttcc
     1621 tcgtcggcgg cgtgatcgcc ctgctgcgcg agagcggcgc gatcgacgcc gtgctgcacg
     1681 gcgcggtcaa gcggctgggg cgcgcgccgt ggatcctgat cgccggcacg ctcgtcgtct
     1741 tcggcctcgg ctcgttcacc atcggcatgg gcgaggagta cgtgccgctg atcccgatcc
     1801 tcgtgacgat gtgcctggcg atgaagctcg actcgatcgt cgcgatgggg atgatctggg
     1861 tgccgtacgc gatcggctgg gcctgcgccg gcaccaaccc gttcggcgtg gtcatcgcgc
     1921 agggcatcgc cggcgtgccg atcacctccg ggctcgccac ccgctcggtg ctgctcgtgc
     1981 tgttcctcgc catcggcttc cagcacgtct acgcctacgc gcggcggatc cagcgcgacc
     2041 ccggggccag cctggtgtcg gacatcgact actcgaccgg cttcgacatg ccgcacgacg
     2101 tgcggctcga ctggcgacgc atcgtgatcc tgacgaccct cgtcggcggc atcgtcggct
     2161 tcgtctacgg cgtcaagacc tggggctggt acatcgtcga gatgaacgcg atcttcctcg
     2221 gcatcggtct cgccgcggcg gtcctgtcgc ggatgacgcc caacgccgcg gcgcagacct
     2281 tcatccgcgg cgcggcggag atgaccgcgg ccgcgttgtt gatcggcttc gcccgcacga
     2341 tcgaggtcgt gctgagccag gggcagatca tcgacacgat catccactcg atcgcgaagt
     2401 cgctcgaggg gagcggctcg tacgtcgcca ccctcggcat gctcggcgtg cagacgatct
     2461 gcaacttctt catcccgtcg ggcagcggcc aggcctacgt gacgatgccg atcatgtcgc
     2521 cgctcgcgac gttgaccggc gtgccgcagc aggtcgcggt gctggcctac cagttcggcg
     2581 acggcttcac caacatggtg gtgccgacga gcgcgctggt gatgggcacg ctggcgctgg
     2641 gcaagatccc ctacacgcgt tgggtgcgct tcgtcacgcc gttgctgatc aagctgttcg
     2701 tcgtggcctg catcgtgctg ctggccgcgg tccgcttcgg cgagagctgg ggcttctact
     2761 gagccgagcg cgcccgccgc tcacgagacc gacggctgcc ccgccgcgcc gcagatctcg
     2821 accgcggcgg cgagcagcgc gcggtcgccg cccggcgggc cgatcagcga caggccgacc
     2881 gggtagccgt cgacctgcat cagcggcagc gtgatctcgg ggaagccgag catcggcgcg
     2941 atacccgaca gctggaagat gtgctggcgc agctcgggcg cgtcgcggtc gctgataccc
     3001 ttgagcggcg cgatcgacgg cgtcgtcggc agcacgaagg cgccgtcgtc gccgagcagc
     3061 tcgcccgcga cgcgctgcat cttcgcgcgg aaggcggcgg ccgccgtcgt gtcggcgccg
     3121 gcgcgggccc gcaccgtctc gaaccgcgcc gccgtcttcg agccgaaccg cggccgcgtg
     3181 cgctcgatcc actcgccgtg cgcgcgccag atctcgggcg ggcgcagcgt gtcgtaggcc
     3241 tcgagccagt cgcggtagcg cagacggctg aggccggcgg gccggaccgg gccgatgcgc
     3301 gacgtcaccc gctcgagcgc cgcctcgaac gcgtgccgcg tcgcgctgtg ggcgatctcg
     3361 agcgtctcct cgagcagcag cagccgctcg atcgacgacg tctcgtcgcc gccgcccggc
     3421 agcagcacgc ggcccacccg ctcgagcaga ccggcctcgc gcgcgaacca acccggcgtg
     3481 tcgaagctcg gcgcgagcgg catcacgccc gcggacgaca gccgcccgtg cgtcgggcgg
     3541 atgccgaaca gtccgcagaa gctggccggg atgcgcaccg agccggccgt gtcggcgccg
     3601 agcgcgaagt cgacctcgcc ggcggcggtc gccgacgccg agccgctcga cgagccgccc
     3661 ggcacgcgat cgggcgcggc cgggttgacc ggcgtgccgt agtgctcgtt ctcgccgaac
     3721 gggctgaacg acatctcgtc ggcgatcgtg tagccgacga ggacggcgcc ggcgtcgaga
     3781 caggcctgga cgacgggcgc ggtccgcgcg gcgggcccgt ggctcgcgag ccagtcgggg
     3841 ctgccgccgc cgacgcggcg gccggcgacg tcgaagacgt ccttggcggc gaagcgcagc
     3901 ccgccgagcg gaccgccggc ggccgcctcg ttgcgcaact cgaaacgctc gcgaaaagcg
     3961 tggacgtcgg ccatcgctag cggtcgagct cggcgcgcag ccaggcccgc gcgaagcgca
     4021 ggtcgcgctc gacggtcgcc agcgaaacgc ccagcgcttc ggcgatctcg ggttgggtca
     4081 gcccgccgaa caggctcagc tcgagcgtgc gcgatttgcg ctcgtcgctc gcgcgcagcc
     4141 ggtcgagcag ctcgtcgatc tcgatcagct tgtcggcggc gtcggggacg acggccatcg
     4201 cctcggccag cgtcacgcgc tcgccgccgc tgccgcgctt ctcgcgccgc cgggcacgcg
     4261 cgtagtcgac cagcacccgc cgcatcacgc gcgccgacag gctgacgaag tgggcgcggt
     4321 cgtgcaccgg cagctccgcg ccggccagac gcgcgtaggc ttcgtggatc agcgccgtcg
     4381 cctgcagcgt gtggtcgcgc cgttccgagc gcatcagccc gaccgccagg cggtgcagct
     4441 cgtcgtagac cagcggcatc aaccggtcga gcgcgcccga gtcgccgtcg cgccagcggc
     4501 gcagcagcat cgtgaccgtc tgcgggtcgt cgcggctcat gccgccaata tggtagccga
     4561 tcgcggcgat tcgggtccgc ggtggtgcgg ggcggccgcc cggcgtatgt tggatgcatg
     4621 cgttccgccc gcctgctgcc gctcgtcctg ctctgcctga ccggcccgct ggcggccgcc
     4681 gacgagggcg aggtctacgt ctacaccaac gccgatctcg agcggctcgg gccgctcccg
     4741 gccggcgagg ccgagccgcc gaccgcggac cagctcgccg agagctgggc cttcgtccag
     4801 tcgacgatcg acgacgcgca cgcgcggatc gacgccgacc gcggctacga gctcgatcgg
     4861 cgcttgaccg aagccgaagc cgacgcgctc gaccgcgccg gcgggtcgcc gcgctacacg
     4921 ctgccctaca actacgcctt gggctacccg tacggcttcg gctaccacga ccggggccgg
     4981 ggcgatcggc tgcgccgcgc ggcctcgcgt ctgtgggagc ggcccaacgc cgagctgttc
     5041 gagccgatcg tgccgatcca cgcgcgaccc taccagacga acctgctgcg ggcgaagacg
     5101 tccggcaggc ggggcttcga ccgacccacc cgacactgac gcgcccgccc gcgtgtttgc
     5161 gggtatgctc cccgtagtct gggaggtcga cgcggatggt cgacgaagac ggcgaccgac
     5221 tcgatccggg ccaacggatc gaccgctacg agatcgtgcg cgtcgagggc tcgggcggca
     5281 tgggcgtcgt ctaccgcgcg cgcgacacgg tgctcgaccg cgacgtcgcg ctgaagcgcc
     5341 cggcgggcga gctgtggcaa agccccgagt cgctgcggcg gttcgcgcgc gaggcccgcg
     5401 cggcggcgcg cctgtcgcac ccgcacatcg tgcaggtgca cgaggtgctc gagcacgacg
     5461 gcgtgccgtg gctctcgctg cagttcgtgc cgggcaagag cctggcggcg cgtctcgcgg
     5521 ccggcgagcg gccgagcatc gaccgcgtga tcgaatgggc cgagcagctc gccgcggcgc
     5581 tccgccacgc gcacgagcgg aaggtgctgc atcgcgacgt caaaccggcg aacgtgctcg
     5641 tcaccgacga cggccgcgcg ttgctgaccg atttcggcct ggcccgctcg ctgccgcggg
     5701 gcgaggccga ctcggtcgag cggcccgcgg gctcgcccgg cgtcacgctc gacgaccgga
     5761 tcgccggcac gccctcgtac atgtcgcccg agcagatgct cggtcgcgag ctcgacgagc
     5821 gcagcgacct gttctcgctc ggcctggtgc tgtacgagat ctgcagcggt cggccgttgt
     5881 tcgcgcccgg cacggcgcaa gaggcgcgcg aggcgctgct cgtccattcg ccgccgtcgg
     5941 tgcgggccgc gaaccgcgcc gtgccgcgcg cgctcgaccg cgtcatcggc aagctgctcg
     6001 cctggcggcc ggaggggcgc ttcgccgacg ccggtctgct cgtcgccgag ctgcgccgct
     6061 gtcgcgaaga gcgcgcgagg cggccgcgcc gggtgtggct cgcggcggcc ggcggcgccg
     6121 ccctgctgct cgcgctcgcc gtcctcggga gcctgcgctt cgccggcggc ccggcgggcg
     6181 agcgccgggc gccggacgtc gtgcgcgcga tggcgctcgt cacctggccc ggcggcgagt
     6241 gggaggcccg ggtctcgccc gacgggcact tcatctcgtt cctgtcgaat cacgacggcg
     6301 ggcagcggct gtggctgcgc gacgtgacgg gcggccaggt cgagccgctg accgcggggg
     6361 aggcgtgggt cgcgagccac gtctggtcgg ccgaaggtga cgagatcgcc tacctcgtcc
     6421 gcgcgcccgg tggcccgcgg ctcgagatcg tcgcgccggg gggtggcccg gtccgccgtg
     6481 cggccgcgct cgacgaccgg ctggacggcg cccgcttgct gcgattcctc ggcgatcggc
     6541 tgtacctcga gaccgccgcg aacgagctcg tcggcttcga tctcgcacgc ggcacgcacg
     6601 cgacgctgat cgacggccgg gccgaggggc ggcatcgcgc cggcttcgac gtgcgacccg
     6661 acgggcgccg ggcggcctgg gccgagcgcg gtgcgtcggg ctgcgcgatc tggaccggcg
     6721 cgctcgacgg cggcgacgca caggcggtca cctcgggcga ccactgcgac gtcgacccgc
     6781 tgtgggtcgg cgccggcgag ggtctggtct acacgtcgag ccggagcggt cagctcgatc
     6841 tgtggcgcgt cgagccggcg ggcggccgcc ccgagcgcat cacgctcagc gccggaagcg
     6901 aggtcgccac cggaagctcg gccgacggct cgctcgtcgt cttcgtccag cgtagcgaga
     6961 tcgccaatct gtggatgtac gtgccggcga agcgactgca tctgcagctg tcggccgacg
     7021 cgctcgggga cgcgctgccg gcatccgtgc cgacctcgtc gatcatcgcc ttcgagcgca
     7081 cgcgtccgcg tctcgacgag tcgaccggcc tgttcggcgc gcagatcctg atcggccgtc
     7141 tggccggcga gcggatggtc gagtcgaagc aggtcgcggc cgagggcgag caggcgctgc
     7201 tcgcgcccga cggccagtgg gtggcctacg cccgcggcga cgagctgtgg gtgcaggacg
     7261 tcgccacggg tcacgcggtg cgcgtctcga cgcgctaccg gcgccccgcc gtcggccggt
     7321 tcccgctcgc gcgctcgcgc gagaacgccg tctggctcga gcgtgggccg gcgctgtgct
     7381 tcgtcgagta cgcgggcggg gggacgcgcg tcgcgcgcta tcccgccggc ggcgccgcgg
     7441 gcgaggtgcg gccggtggcc gagacgcgcg acggggtggc gatcgacgac ctgtgggcgt
     7501 cgcccggcgg acgacgcctg gcgtggatcg agcgcgagca cggcacgagc cgcctgcggc
     7561 ttcacgacac ggacggtggc gcgagccgga cgctcgtcga cgaggacggg ccgttgtggc
     7621 tcgacggctg gatcgacgac cgatcgctgc tcgtcgtgcg cgacctgccc gccgccggtg
     7681 acgtcgcgcc gagcatcgcg ctgtcgcgcg tcgcgctcga cggctcgatc gcttcgctcg
     7741 gcgaggtcga gcgcgccttc gtcgacaccg tccgtctcgt ccccgcggcg cgccgcatct
     7801 acctgacggc cgccggcgac gatcgcgtcg acaacctgat cgccgtctcg ctcgacgacg
     7861 gttcgcgcga gaccttgacc gacaacaccg tgaccgacgt caccttctcg acggtcgcac
     7921 cggcgccgga cggcgcgctg atctacacgc gccaacagcg ggtcgccgac ctgtggggga
     7981 tcgagttccg gccgcagtga gcgaccgcaa cccgtttgac cctccccggc gaggcgcgta
     8041 ccatgccgga aagaggggtg ccacgacaaa ccgggtcgag cgatgatgga cgacggttcg
     8101 gacatcggca tcgacgtgcc gctgccgtcc gacgggccgc cggagccgcg ttaccggccg
     8161 ggcgaccggg tcgcgcgcta cgagatccgc ggggtcgtcg gggcgggcgg aatgggcgtc
     8221 gtctatcgcg ccctcgacac gcgtctcgat cgcgaggtgg cgctcaagtg cccgtggggc
     8281 acgctcgccg acaactcggc agcgctcaag cgcttcgagc gcgaggggcg ggcggccgcg
     8341 cgcttgttcc acccgaacat cgtcgagatc cacgaggtgt tccagcacga cggtctgccg
     8401 tggctcgcgc tgcagttcat cgagggcgag agcctgcgtg ccaagctcga gcggaccggt
     8461 gcgctgccgc tcgaggacgt gctgtcgtgg gccgagcagc tcgccgccgc gctcgagcac
     8521 gcgcacgcgc agaacatcct gcaccgcgac ctcaagccgg ccaacgtgct ggtcacgccc
     8581 gacgggcgcg cgatgctgac cgacttcggg ctggcgcgtc cgatcaagac gagacccgat
     8641 gccggcggcg aggcgagcgg caccgtctcg ggcacgatca cgctcgacgg ccacctcgtc
     8701 ggcacgccgg cctacatgtc gcccgagcag gcgctcggcc ggccgctcga cgggcgcagc
     8761 gacatcttct cgctcggcgc gctgctgtac gagatgtgct gtgggcggcc gttgatcgtc
     8821 aagggcagcg ccggcgacgc gcgctcggcg ctggtcggct tcgagccgcc gtcgatccgc
     8881 gagctcaacc cgcgggtgcc gcacggcttc gagcgcatcg tgcggaaggc gctggcctgg
     8941 agaccggagg accgctaccc ggacgcggcc gcgctgaaga gcgacctcgc cgactaccgg
     9001 cgtgccgcgg cgcgctggcc gcgctgggtc ccgccggccg cggccgccgc ggtcgtcgcg
     9061 ttgctcttgg ccgccgtgct gttgacgcgc cactactcgc gggccgagcc gaccgacgtc
     9121 gggccgggcg aggtcgccgc cgaggcgctc gtcacctggc ccagccggga atcggacccg
     9181 aagctgtcac ccgacggccg gtgggtttcg ttcctgtcgg accgcggagg ggtgccgcag
     9241 gtctggctgc gcagcctgac cggcacgctg gtcgaggagc tgacggcggc ggcggcggcg
     9301 gtcaccgcgc acgcctggtc gccggacggc cgcgagatcg tccggctggt caaccacccg
     9361 gacggcccgc tgctgcaacg aatcccggcg ctcggcgggc tgccgcggga cagcgtcacg
     9421 ctcgacgagc ggttcgccgg cgcaaggctc gtacgctgga tcggccatcg cgtctacgtc
     9481 gagctcggcg agcgctcgtt ggtccgcgtc gatctcgaga gcggcgagac cacgccgctg
     9541 atgaccgact cggccgccgc cggtctgcgc tggggcttcg acgtgctccc cgacgagtcg
     9601 cgcgtcgtct actcgggcgg ccgcgacttc cgctacgcga tctggaccag cgatctcgac
     9661 ggctcgaatg tcgagcagtg gacgacgggc gagtactgcg acacctcgcc gcgcttcctc
     9721 gacgccgagg gccggtacgt cgtcttcacc tcgacgcgca gcgggcagaa cgacctgtgg
     9781 cgggtcgcac tcgacgatcg cgcgctggtg ccgatcacgc tgagcacgag caccgaggtc
     9841 gtcggcgacg tctcgcggtc cgggcacctg atcgtcgtcg aggagaaccg cgacgaagcc
     9901 cacctgtggg cgctcgatcc gcgctcggcc gacgcccggc agctcacctc ggacgcgctc
     9961 agcgactcgt ggccgtcgct cggcccgggg tcgccgtttc tggcgttcct gcgcaggctg
    10021 ccgcggctcg acacgacgac cggggtgttc gaatcgcgcg tgctccgcgg ccgtctcgac
    10081 gacaccggtc tcgtcgagca cgaggagatc gccgagaccg gcgcccagcc gctgctctca
    10141 cccgacggcc agtgggtggc ctacctcgtc gggcgcgagc tgcgcgtacg ccacgcgccg
    10201 accggccgcg tcgtccacgc gagcgacggg ttcgtcgcgc cggcgagcga tctgtacctc
    10261 tacgccaacg tgacgacgaa cgccgtctgg catccgcacg accctcggct ctacttcgtc
    10321 gatcgggacg acagcggatt cgtcgtcaag cagatcgacc tcgcacggtc ggggcccgcg
    10381 gaggcgctgg gctcgtacgg ccgcgacacg cgtgtcgacg acctgtcggt cgcccccgac
    10441 ggcggtcacc tggcctacgt ctcggagtct cgcgacgtcg acggcttcga ggtggaggcg
    10501 atcgagctgg cgacgggcga ggtgcgcggg ttgctgcacg tcgacgactg gcgaccctac
    10561 accaagggat ggctcgacga ccacacgctg ctggtggccg ggacaccgga gggccgcggt
    10621 cggggaacga tcttcctcca cgcgctcggc ctcaacggcc acctgcgcga gctcggtcgg
    10681 ttcggaccgg gtttggtcaa cacgctgacc ttcgacccgc gggcgcgaac ggtctacgcc
    10741 accgtcgagg acgaggcgcg cctgcagaat ctcgtcgcct tctcgctcga cggcggcggg
    10801 ccgcggcggt tgaccgacaa ccgtctgccc gacgccggtt tctcgacggc acagcttgcg
    10861 cccgacggct cgatcgtctt cgtccgcgcc aagcacacgc aggacatctg gtcgatcgag
    10921 ttccgccggc gctaacgcct tcgcgctact tgcactgccc ccagtcgcac ggccgcgcct
    10981 ggcacttcat gcaacagcag tagggttgcg ccggcgagca ggagcacttg atcagcacct
    11041 tgagctggtc gatcacccgg tcgagcagcg gcaggatcgt ctcgtagctc gccgggtctt
    11101 cggtgtattc cttcgtcgtc acgagctcga gattcgtgac gaacagcttg atcgtctcga
    11161 tctcccacca ctccgccttg ccggtgtccg gcggctcgtc gtgcttgccc ggctcggtgt
    11221 gcttgccggg ctcgccgtcg aggtcgttca gcgcctgggt cagcgccgcg atgatcgcgt
    11281 cgtaggtgtt cgagtccgac atgtaggccg aggcggccag cgactcgatc tcggtgatgc
    11341 gcagccggat cttggccagc tcgcccgccg agaactcgtg ccgcaccggc tcggccgggt
    11401 cggacaggac gacggcggtc acgatcaacg agagcaacag ggagcccagc gcgaccttgg
    11461 tcgcgcgacc ggcgtgtttc atgacatacc tccgtgtcga gacgaggccc cggcgatgtg
    11521 aagactggtc gtacccctcg ggctcgaacc cgatctgttg ggagcagcat agctcaggtg
    11581 acggacggcc ggggggctat tcggcgctca gacgtcgctc gagctcctgg cgccagttga
    11641 cgagaaaggt cacgccggtc gggcggtcgt cgtcgcgctc ggagcggatg atcagcaccc
    11701 ggccgtcggg cgcgacgtcg tagtcgacgt tcagcaggcg cgacgggaac cccccgacga
    11761 ttttgcgcgg ccggccggcg cgcagacggc cgtcgcccgc ttcgacgtcg gcgaccagga
    11821 tctcgtcggc ctggcggaag tagagccgct tgccgtcgcg cgaccaggtc gggtgcagcc
    11881 cgccgtcggt cgagacctgc cacttgccgc cggggcccgg gtggggtcgg acgtagacct
    11941 cggcgcggcc cgattcgtcg gaggtgtagg ccaggaaccg gccgtcgggc gagatcctcc
    12001 cggtgtgctc gccggccggc gtcgcgagca gcacccgacg ctcgccgtcg accagatcga
    12061 ccgccatcag attgccggcc cgctcgtcgg tgaagtcgat cagcaccgtg cggccgtcgg
    12121 gcgcccaatc ggtcggcacg gccttcccct ggtgcgcggc gacgtccggc gcgtagacca
    12181 gctcgtccgt cccgctgccg tccgcgttct tggcgtacgt gccaaccgcc gcattgttgc
    12241 ggaccgacgc gaacaggatc cgcgtgccgt cgggagacca cgtcgggaag gtgttgtcgc
    12301 cctcgaaggt caaccgcgtc gacgtctcgc gttcgatgtc gtagatccag atgtcgaagg
    12361 tcgacccacc ctcgatcgcg acggcgatcg accggccgtc gggcgagatg cgcgggtcgg
    12421 tgtaacccgc gacgggcgcg tcgatcggct cgatgcggcc gtcgggctcg agccacacca
    12481 tccgcgactc gcgcgagacc gactcgccgg cgacgtaggc cagcagaccg tccgcggcga
    12541 agtcgaagtg gacgatgccc gacgagcgca tgcccatcac gtcgtggacg accggcacgg
    12601 gcgtgccgcg cgtcgcgagc cgctcgacgt cgaaccgcgt ggcgaacagg aagccgtcgc
    12661 gcgagaacaa caggtagccg cccgggatcc agcgggcgat gctggcgccc tcgagcacgg
    12721 tcctgcgctc gccggtcgat gggcgcagcg cctcgatccg cgcgccgtcg tagttttcgg
    12781 ggctgtcgag cgtgccgacg gtgaacagca ccagatcgag ctcgggcagg tattgcggcc
    12841 agcggtgcgt gcgctcgccg cgatcggcgc ggatctcggt cagcggctcc ggccggcctc
    12901 cgcccgcgcc gaccaccgac agcggcatcg tgtagtgcgc gctgaacacg atgcggtcgt
    12961 cggcgcccca gctcgcgccg cggttgttgc ccgacgcttc gcagatcgcg atcgggttcc
    13021 cgccgtcgac cgggaccttc cacaccttgt tgttggcgaa gaagccgacc cactgcccgt
    13081 cgggcgagaa gaacggcgtc accgcgctct cggtgccgcc gacgacgacc ggctcgccgc
    13141 cctcccgcag gtcgcgcagc agcaggcgcg gcacggcgcc gtcgttcaag ctcagcgcca
    13201 gcttccgccc gtcgggcgag agggcgatca cccccatctg ctggtcgtcg atcgagaggg
    13261 cgtccgggaa cggggcgacg agcgagagca acggggcggg ggccgggggc acccgcgagc
    13321 cgcgccacga accgacggcc gcgacgccga gcagcacgac gagcgtccag ggcaggacgc
    13381 gggcccacgt ggcgcgacgc gcgggtcggg gcgcggcctc gcccgcgcgc tccgggtcga
    13441 cgccgtcgcg tgtctcgctc atcggcagca gcgcgcccgc gaggtgggcc agacgccggt
    13501 cggggtcgcg cgtcaggcaa cggcggatca gccggcggat cgaaggcggc gtgtcggcgg
    13561 gcaggcgatc ccagtccggg tcgaccttga ggatcgaggc cagcgtgtcc gagaccgtct
    13621 cgccgtcgaa cgcccggcgg ccgctcagca tttcgtacag cacgcagccg aaagcccaga
    13681 cgtcggcccg gcggtcgacg gcccggccgc gcgcctgctc cgggctcatg tagcccgccg
    13741 tgccgagaat cacgcccgcg accgtgccgg ccgaggtgat cgtcggcgac agcgccgggt
    13801 tgccgctcgt cgtctccggg ccgaccccct tggccagacc gaagtcgagc accttgaccc
    13861 ggcccgcgga cgtcagccgg acgttggccg gtttgaggtc gcggtggatc acgccgtggt
    13921 cgtgcgccgc ctcgagcgcc cgggcgatct ggatcgcgat gtcgagcgtc tcgtcgagcg
    13981 cgagcggccc gcgcgcgagc cgcgtcgcca gatcctcccc gtcgaccagc tccatcgcga
    14041 ggaagcgcag cccctcgtgc gcgtgcaccg agtagaccgc ggcgatgttg gggtggttca
    14101 acgaggcgag cagccgcgcc tcctgctcga agcgggccag ccggtccgcc gacccggcga
    14161 aggtctcggg cagcaccttg atcgcgatct cgcggccgag gctctcgtcg acggccttcc
    14221 agacgacgcc catcccgccc tcgccgagct tctcgacgat gcggtagtgc atcaactgcg
    14281 tgccgggttc gatccgcacc ttgcccctcg accgccgccc agtctactcg gagtcgacgc
    14341 ccgccgcctg tttgacgaac tcgggcgtca gcggctcgcc gcggaagaac tcgccgctct
    14401 tgccccgcgt cagcgcccag cggtgcagcc agctgatgcc gcccgcgccg aagtagtcgt
    14461 ccggcgagcg gtaggcctcg tcctcgagca cctcgtcgag gccgacgaag acgtagccgc
    14521 gagcgcggat cgccacggcc aggcggccga agtggtcggc gttgagctgg ttggcgtgga
    14581 tcagcaggac gtgggggatg gcgcggccga acagctcgtc ggtctggcgc tcgaagtagg
    14641 cgaacttgcg gtccatgtag ccgacgtagc cgtcggcgat gcgcttcatc gacgtttcgt
    14701 cgtcgcgcga ctgcgccagg tcgtaggccc gggcgaagat ccactcggag ttgtcgatcg
    14761 tcaccggcgc gacgcggtag ccgtggcgat cgagcagggc gtggaccgcc tcgcgcgtct
    14821 cgcggtcgcg gccggtgtgc aggtacgggt ggcggaacca gaccagcctg cgctcgcgcc
    14881 cggcgagcag cggccgcgtc acgcgctcgc cgcggacgac gtcgtcgagg aaggtctcga
    14941 gctcgacgcg atgcaggtcg gggtgcgaga aggtgtggtt gccgagctcg aaaccggcgt
    15001 cgagccacgc cacgagcagc gccacgcgct cggggtcgac ctcgtcgccg gcgtggagct
    15061 tcttctcgtt gacgaagccg acggccggca cctcgtgctc gtcgagcgcg gcgagcagat
    15121 cggtggtgat gcggcgccag gcggcgttgt cggtgagcgt cggcgagacg accggcaggt
    15181 cgtcgatcgt caccgcgacg cgccgggcgg ggccggccgc gaacagcgtg ccggccgcga
    15241 agacggccgc cgcgagcgtg atcgggatcc tgagcatcgt cctctcctcg catacggcga
    15301 agtcgggccg gacagggtac gctacaccga tgccgcccga accgcacgcc ggcgacacgc
    15361 gcagtcggct gacgctgatc gcctcgctct acgtcgccca ggcgatcccg ctcggcttcc
    15421 tgatcgtcgc cgtgccggcc atcctgcggc gcgaggggct ggggctcgag caggtcgggc
    15481 tgatc
//
LOCUS       DASPGN010000251         5432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_394658, whole genome shotgun sequence.
ACCESSION   DASPGN010000251 DASPGN010000000
VERSION     DASPGN010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394658"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VD788_07345"
     CDS             complement(<1..366)
                     /locus_tag="VD788_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76119.1"
                     /translation="MKTIVGISILLMIATVAGCSSDDASSPSDSLSPMTVRLNVMTNP
                     VGCAANVYWESSPDGMTGEMIRMTSPAASNPEVVSNGTTISVNGNMSCAVPGSSGSVD
                     ATLQMMVNGNWQDAPMTGTH"
     gene            672..1004
                     /locus_tag="VD788_07350"
     CDS             672..1004
                     /locus_tag="VD788_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76120.1"
                     /translation="MRKKTAWAVVLILVGLCLAAGTVGAQGTATVKGKAVKVDAKAGT
                     LTVVEEPRAGAEKKESTFRVIADTKIVRDGDPIDLGQVSSGDVVVVVYKVDKGDMVAV
                     SVGVSKAA"
     gene            1119..2177
                     /locus_tag="VD788_07355"
     CDS             1119..2177
                     /locus_tag="VD788_07355"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HXV76121.1"
                     /translation="MRAVLKGTGMFVPELVVDNHRLSRIMDTSDEWIQQRTGIVTRHY
                     AGPHQATSDLAVPAAAQAVAEAGLERDQIDYVVFATMTPDYYFPGAGPVFARKFGLAG
                     VPCLDIRQQCAGFVWGLQLADAVIRAGQYRNVLLIGAEVHAGFMPWDWDLLFGKSRRE
                     PTASEYEWNTRFRDRTVLFGDGAGAFVLSAVDDDETEAGIEAVTVHSDGVHWDKMWVD
                     GVGSAYRPWIDRRMIESGAVVPIVQGRDVFKVAVTLMPDVTQKILAQHGYTLDDLRLV
                     IMHQANLRINEACQKRLGLPDERVFNNIQRYGNTTAATLPIAFHEARRERGLEAGDLV
                     AFVALGSGLNWGAALYRC"
     gene            2266..3159
                     /locus_tag="VD788_07360"
     CDS             2266..3159
                     /locus_tag="VD788_07360"
                     /inference="COORDINATES: protein motif:HMM:NF003784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HXV76122.1"
                     /translation="MSEFDKQLRKVKTERSFIAALDQSGGSTPKALHLYGIEPDAWSN
                     DAEMFEIMHRMRARIMTSPAFDGDRVLGAILFEDTMDREVAGRPTAEYLWQVKRVVPF
                     LKVDRGLVEQREGVQLMKPIPGLVALLDKAKTKQIFGTKMRSVIQRADVDGIAAIVRQ
                     QFEFGGQILAAGLVPIVEPEIDIHCPDKARAEELLEDKLLEALDELNPDEKVMLKLTL
                     PEEDDFYRGCIAHPNVLKVVALSGGYSRSEANERLARQHGMVASFSRALTEGLTVDQS
                     DEQFNRALDEAIGSIYAASQS"
     gene            complement(3156..3725)
                     /locus_tag="VD788_07365"
     CDS             complement(3156..3725)
                     /locus_tag="VD788_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76123.1"
                     /translation="MSKRLRDAARWRLAGLLMRLPRADRAREIELLAREADHPSLTAA
                     VAAAVDATEGGYLALLGPGGPLSPREVSHRPLEDPGRLLAEIAAYHRAFGYRASAEDP
                     ADHLAAAADFAGYLLMKEAFAERSGLHRAAMTTREARAGFVDRHVRCTVRGMRRALEA
                     SGTPVPWLGAALEAIARLAGTDEAARCSA"
     gene            complement(3722..4600)
                     /locus_tag="VD788_07370"
     CDS             complement(3722..4600)
                     /locus_tag="VD788_07370"
                     /inference="COORDINATES: protein motif:HMM:NF021010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethylbenzene dehydrogenase-related protein"
                     /protein_id="HXV76124.1"
                     /translation="MTKAADRRFLSVVPSALALALLAAACVERHEPLATEVAALRVAT
                     LPEDPTDAIWRDAPLYRAELLLQDLVEPRLLEPSTASVDVRALTDGTHVAFRLEWRDA
                     TRDDMPKAARFSDACAVQLPGATEPDVPDPQMGHAGRPVEITLWRASWQAWTDGRKDE
                     IRSIYPNASIDHYPFEAPSLEPGSPEQQAMEQRYAPARRLGNRMEGPRERSVEDLVAE
                     GPGTLTRAEEQRSLGRGVRTETGWQVMIVRLWPEPLDPGRRSEVAFAVWDGDRGEVGS
                     RKMRTGWVPLAIEGER"
     gene            complement(4610..>5432)
                     /locus_tag="VD788_07375"
     CDS             complement(4610..>5432)
                     /locus_tag="VD788_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXV76125.1"
                     /translation="DAGERIAGWRPAELDYAHPNVGEDDCTGTVDGGAALDLPHAHWF
                     FYLARICNHCTYPGCLASCPRGSIYKRPEDGIVLVDQERCRGYQECVKGCPYKKMFFN
                     PMTGTSEKCIACFPKIEQGLQPQCFENCIGKIRLAGWISPPERSRPDNPIDYLVHERK
                     LALPLFPQLGLEPNVYYIPPTHVPRHYLRMMFGPGVDHAIETYRRIPDDADLDGLLKL
                     FGSSARVLPRWKREGDRVLGLDDSGNELARVPLREPLKVLQAYDVRHGVTRTNCP"
BASE COUNT          904 a         1811 c         1863 g          854 t
ORIGIN      
        1 gtgcgtgccg gtcatcgggg cgtcctgcca gttgccgttg accatcatct gcaacgtggc
       61 gtcgacgctt ccgctcgagc cggggacggc gcagctcatg ttcccgttga ccgagatcgt
      121 cgtcccgttc gacacgacct cgggattcga cgccgcgggc gacgtcatac ggatcatctc
      181 cccggtcatt ccatccgggc tcgactccca gtagacgttg gccgcgcagc cgacgggatt
      241 ggtcatcacg ttgaggcgca cggtcatcgg cgacaggctg tcgctcggcg acgacgcgtc
      301 gtccgagcta cacccggcca cggtggcaat catcaacaag atcgagattc cgactatcgt
      361 cttcatcgag attctcctca acgccccagg gagacctcgg cgagcgaggc catgctgtct
      421 gaggaaaaac tcggtcggag aaaactcggt gtcaattaca tgacgtgctc gatcatggac
      481 gaactcggtc gcatccgaaa tttcggcggc ggctgtggtc gctgagagac gcggcgtggc
      541 accgtgcaca cattcatgta atcggcgcgc gtttttcccg cgctcggccg tccaccttcg
      601 cccgggaatc gcccggcacg cggcttgaaa caggcttcga ccgaaactcg aaccgcatgg
      661 agggaaacgc gatgcgcaag aaaaccgcat gggccgtcgt gctgatactc gtcggattgt
      721 gcctggccgc cggcacggtc ggtgcccaag ggaccgcgac cgtcaagggc aaggccgtca
      781 aggtcgatgc caaggcggga acactgaccg tggtcgaaga gccgcgagcc ggcgcggaga
      841 agaaggagag cactttccgc gtcatcgccg atacgaagat cgttcgtgac ggcgacccga
      901 tcgatctcgg ccaggtctcg agcggcgacg tggtcgtcgt cgtctacaag gtcgacaagg
      961 gcgacatggt tgccgtcagt gtcggagtat cgaaagcggc ctgacccgcg cggcggtgcg
     1021 acccgcggcg taaaagccgc gagtcgcacc accggttgac cgattgacag ccccggcccg
     1081 ctccccgaca atcgacccgt tcgatcgggg gagcgatcat gcgagcggtg ctcaagggca
     1141 cggggatgtt cgtgccggaa ctcgtcgtcg acaaccatcg cctgtcccgc atcatggaca
     1201 cgagcgacga gtggatccag caacggaccg gcatcgtcac gcgtcactac gctggcccgc
     1261 accaggcgac cagcgatctc gccgtacccg ccgccgcgca agccgtcgcc gaggccgggc
     1321 tcgagcgcga ccagatcgac tacgtcgtgt tcgcgacgat gacgcccgat tactacttcc
     1381 cgggcgccgg gccggtcttc gcgcgcaagt tcggtctggc cggcgtgccc tgtcttgaca
     1441 tccgccagca atgcgccggt ttcgtctggg gcttgcagct cgccgacgcc gtcatccgtg
     1501 cgggtcagta ccggaacgtg ctgctgatcg gcgcggaagt gcacgcgggg ttcatgccgt
     1561 gggactggga cctgctgttc ggcaaatcgc ggcgcgagcc gacggcgagc gaatacgagt
     1621 ggaatacacg gttccgcgat cgcacggtgc tgttcggcga cggcgcgggc gcgttcgtct
     1681 tgtccgccgt cgacgatgac gaaaccgagg ccggaatcga ggccgtcacc gtgcactcgg
     1741 acggcgtcca ctgggacaag atgtgggtcg acggcgtcgg cagcgcctac cgcccgtgga
     1801 tcgaccggcg catgatcgaa agcggagcag tcgtgccgat cgtccagggt cgcgacgtgt
     1861 tcaaggtggc cgtcacgctg atgccggacg tgacgcagaa gatcctggcc caacacggct
     1921 acacgctcga cgacctgcgg ctcgtgatca tgcaccaggc caatctgcgg atcaacgagg
     1981 cttgccagaa gcgactgggt ctgcccgacg agcgcgtctt caacaacatc cagcgttacg
     2041 gcaacacgac tgcggcgacg ctgccgatcg cgttccacga agcgcgccgc gagcgcgggc
     2101 tcgaggcggg cgacctcgtc gcgttcgtcg cgctcggcag cggtctgaac tggggcgcgg
     2161 cgctctaccg ctgttgatcg ttccggcgtc gaccggcggt ggcgacgcag gcgacggcgt
     2221 tggattacaa tgcagcccga cgactcgaat cgagaggtgt ggttcgtgag cgaattcgac
     2281 aaacaactgc ggaaagtcaa gaccgagagg tcgttcatcg cggcgctcga ccagagcggc
     2341 ggcagcacgc ccaaggcgct gcacctgtac ggcatcgagc ccgatgcctg gtcgaacgac
     2401 gccgagatgt tcgagatcat gcaccggatg cgcgcgcgca tcatgacctc gccggcgttc
     2461 gacggcgacc gggtgctggg tgcgatcctg ttcgaggaca cgatggatcg cgaggtcgcc
     2521 ggccggccga cggccgagta cctgtggcag gtgaagcgcg tcgtcccgtt cctcaaggtc
     2581 gaccgggggc tggtcgaaca gcgagagggc gtgcagttga tgaagccgat cccgggactc
     2641 gtcgcgttgc tcgacaaggc gaagaccaag cagatcttcg gcaccaagat gcggtcggtg
     2701 atccaacgag ccgacgtcga cgggatcgcc gcgatcgtcc gccagcagtt cgagttcggc
     2761 gggcagatcc tcgccgcggg tctggtaccg atcgtcgagc ccgagatcga catccactgc
     2821 ccggacaagg cgcgggcgga agagctgctg gaggacaagc tgctcgaggc gctcgacgag
     2881 ctgaaccccg acgaaaaggt gatgctcaag ctgacgttgc ccgaggagga cgatttctac
     2941 cgaggctgca tcgcgcaccc gaacgtgctg aaggtcgtcg cgctgtcggg cggttactcg
     3001 cgcagcgagg ccaacgaacg gctggctcgg cagcacggca tggtcgcgag cttctcgcgc
     3061 gcgctgaccg agggtctgac cgtcgaccag agcgacgaac aattcaaccg ggcgctcgac
     3121 gaggcgatcg gctcgatcta cgccgcgtcg cagagctagg ccgagcagcg ggcggcctcg
     3181 tccgtgccgg ccagccgagc gatcgcttcg agcgcagcac cgagccacgg gaccggtgtc
     3241 ccgctcgcct cgagcgctct acgcatgccc cgtaccgtgc agcggacgtg gcgatcgacg
     3301 aacccggcgc gcgcctcgcg cgtcgtcatc gccgcccgat gcaggccgga ccgctcggcg
     3361 aacgcttctt tcatcagcag atagccggcg aaatcggccg ccgcggccag gtggtccgcc
     3421 gggtcctcgg cgctcgcgcg atagccgaac gcccggtgat aggccgcgat ctcggccagc
     3481 agcctgcccg ggtcctcgag cggccgatgg ctgacctcgc gtggcgacag cggaccgcct
     3541 ggcccgagca gcgccaggta gccgccctcg gtcgcatcga ccgcggcggc cacggcggcg
     3601 gtcagcgacg ggtgatcggc ctctcgcgcc aagagctcga tctcccgtgc gcgatccgcg
     3661 cgcggaagcc gcatgagtaa ccccgccagg cgccagcgcg ccgcatcgcg tagtcgtttg
     3721 ctcatcgttc cccctcgatc gcgagcggga cccaccccgt ccgcatcttg cgtgagccga
     3781 cctcgccgcg gtctccgtcc caaacggcga acgccacctc gctgcgacga ccggggtcga
     3841 gcggttcggg ccacaaccgc acgatcatca cctgccagcc ggtctcggtg cgcacgccgc
     3901 gtccgagcga acgctgctcc tcggcccggg tcagcgtgcc cggtccctcg gccaccaagt
     3961 cctcgaccga acgctcgcgc ggcccttcca tgcggttgcc caggcggcgc gccggggcgt
     4021 atcgctgctc catcgcttgc tgctcgggcg agccgggctc gagcgacggg gcctcgaacg
     4081 gataatggtc gatgctcgcg ttgggataga tcgaacgaat ctcgtccttc cgaccgtcgg
     4141 tccaggcttg ccacgacgcg cgccacaagg tgatctcgac cggacgcccg gcgtggccca
     4201 tctgcgggtc gggtacgtcg ggctcggtcg cacccggaag ctgcacggcg cacgcgtcgg
     4261 agaatcgcgc cgccttgggc atgtcatcgc gggtcgcatc ccgccactcg agccggaagg
     4321 caacgtgcgt tccgtcggtc agcgcacgca cgtcgacgct cgccgtcgac ggctcgagca
     4381 ggcgcggctc gaccagatcc tgcagcagca gctcggcgcg gtagagcggc gcgtcgcgcc
     4441 agattgcatc ggtcggatcc tcgggcagcg tcgccacgcg cagggcggcg acctcggtcg
     4501 ccagcggttc gtggcgttcg acgcaggccg ccgccagcag cgcgagggcg agcgccgacg
     4561 ggacgaccga gagaaaacga cgatcggcag ctttcgtcat caccttctcc tacgggcagt
     4621 tcgtgcgggt cacgccgtgc cgcacgtcgt aggcctggag caccttgagc ggttcgcgca
     4681 gcggtacgcg ggccagctcg ttaccgctgt cgtcgaggcc gagcacacgg tcgccctccc
     4741 gcttccaacg cgggagcacc cgcgccgacg agccgaacag cttcaatagt ccgtcgaggt
     4801 cggcgtcgtc ggggatccgg cggtaggtct cgatcgcgtg atccaccccc ggtccgaaca
     4861 tcatccgcag gtagtgtcgc ggaacgtgcg tcggcgggat gtagtagacg ttcggttcga
     4921 ggccgagctg cgggaacagc ggcagcgcca gcttgcgctc gtgtaccagg tagtcgatcg
     4981 ggttgtccgg ccgtgagcgc tcgggcggcg agatccagcc ggccaggcgg atcttgccga
     5041 tgcagttctc gaagcactgc ggctgcaggc cctgttcgat cttcgggaag caggcgatgc
     5101 acttctccga cgtgccggtc atcgggttga agaacatctt cttgtacggg cagcccttga
     5161 cgcactcctg gtagccgcgg cagcgctcct gatcgacgag gacgatgccg tcttcgggtc
     5221 gcttgtagat cgagccgcgc gggcacgacg ccaggcagcc ggggtaggtg cagtggttgc
     5281 agatgcgggc gaggtagaag aaccagtgcg cgtgcggcag gtcgagcgcg gcgcctccgt
     5341 cgacggtgcc ggtgcagtcg tcctcgccga cgttcgggtg ggcgtagtcg agctcggccg
     5401 gtcgccagcc cgcgatccgc tcgccggcgt cg
//
LOCUS       DASPGN010000252        19423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_394667, whole genome shotgun sequence.
ACCESSION   DASPGN010000252 DASPGN010000000
VERSION     DASPGN010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 19423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19423
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394667"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VD788_07380"
     CDS             <1..786
                     /locus_tag="VD788_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV76126.1"
                     /translation="EGARLYRDQKVIVVMPAYNAARTLYTTYAEVMAQDYVDAVIVVD
                     DASKDATVDIAQGLARVTVHRHDANRGYGANQKTCYRLALDAGADIVIMVHPDYQYTP
                     KLIPAMTSMIGNGLYHCVIASRILGGYALRGGMPWWRYVSNRVITFYENALIGAKLSE
                     YHTGYRAFSRRLLEALPLEANSDDFVFDNQMLAQILWMGFVVAEVSCPTRYHAEASSI
                     DFRRSVRYGFGCLWTATQFFLAKHDWAGLRRFPRELRETGARG"
     gene            783..2591
                     /locus_tag="VD788_07385"
     CDS             783..2591
                     /locus_tag="VD788_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76127.1"
                     /translation="MSGLTGTRRSIGFAPSLVVILLIGAVLRAGYTIAQARSDPWFAL
                     PMLDGRYYVEWAASLAAGRGGPPGAFYLAPLYPYLLALLIRLGGLSFLGLYVVQQAMG
                     LASAAILAVGGRRVLGETAALATALLFVLHHPLQFFAARPLGEALAILLLTAALALVW
                     RTGPRAGLAAGVCLGLAALTRPNLLLVLPAWVVVESRDRRWRRLATLVLGFAVVVAPV
                     TLRNRIVSGHWVAISSNGGLTAYHGNGPGARGVYTHPAGFPFEMHQQREAATRRARQL
                     SGLALDPVEADAWWGRKALEARLARPLDTAGLVAWRVALTLDNRELSLDYPPMLDPNP
                     WRTTLRLPPGHELAWVPWALLVGLAGAGAVGAGWRRSGGAHAWGALIAALATPVLFYV
                     SSRYRLPAAALLAVPAGAGVAVLLERSVGARRWRALAMAVALIALSMLVPSAALERTQ
                     RAEGLGNRAAAYLACGQLERAEADATRAVELEPDAPRLWATLGAVMDRRERFAAAEQA
                     YERALALEPIPEAAAGLAAALAAQGRVDRGVAVLRRALVLAPGNAACWNGLVGLLYAS
                     GDLAAARRTAREAVAAGVELDGELRSLAEGGAEDGS"
     gene            2581..3084
                     /locus_tag="VD788_07390"
     CDS             2581..3084
                     /locus_tag="VD788_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76128.1"
                     /translation="MARRSTRGIVGLAASAVLTGALGCSPTVTDTGFAGTWASEVQVG
                     GRSVVAIWRDGSGYRFAVNRYDADGRHHLRCTAEGECAYFDGEAPYYALEFAVRAGSN
                     DGALYVDCKGHPLEGKEGTPIEWVERLSVEPGGIVLTAQRIELNGTPVTAMPRRFAKQ
                     SDRPRFR"
     gene            3081..3800
                     /gene="rnc"
                     /locus_tag="VD788_07395"
     CDS             3081..3800
                     /gene="rnc"
                     /locus_tag="VD788_07395"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXV76129.1"
                     /translation="MSRPSIDRTAGGLERALGYRFRDRTLLERALTHRSHVHEREGET
                     GESYERLEFLGDALLGMFVADWLYRKDPRAGEGTLSRGRQTVVRASSLARIASALGLG
                     DAILLGRGEELTGGRLKRSLLADVFEAVVAAIYLDGGLRQARAFVHRHLGPELGRAVG
                     PSPASDDFKTRLQEVVQGTLQRTPSYRIVSTAGPAHALSFVVEVLVGDEVLGRGRGSN
                     RKRAEQEAARCALDRFESTDR"
     gene            3797..5266
                     /locus_tag="VD788_07400"
     CDS             3797..5266
                     /locus_tag="VD788_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76130.1"
                     /translation="MKGDSRNPVPVTRLCGMLALCGLSGAVSAEEPVLLNDRSGGQIV
                     IQAETELSIEGFSGTVAVRAGKSGELRYEIRALADRNQPRALALWSTKGVLRLVPIEP
                     PATEPLLVEIAVPPALAVNVDLSDSKVEVSGLNSPVGVTGARLELDIRGIAGTVDLAV
                     SEATVRIDGIAGDVSFEGESVRLDLRRVQGSAGLTVQGGEVTLAEVSGETDADLGETV
                     FNAVALVHGARVQAAGGRISLGQVSQQVELVVEGTPVEISGASGRVTVDSDSEVTLNE
                     VQGTVILTGYGGVIRGTASSGPLEITIDDADVTLEQWQGKIDVRGDGLDLAMRQITGS
                     IAAATTSSNFVIERVEGPVTIENEFGDVRVSGVSGTTKIVNRDGITVAADLNGQLDLH
                     GSGERVEVSWAQLGSDANHVIENAAGQVVLRVPTNARCRFDVRSEFGEVNSQLPGVQV
                     SDDGTFASGALGGAEQPTIQVRSSGDVELSQYSPPAPTQ"
     gene            complement(5260..6417)
                     /gene="moeB"
                     /locus_tag="VD788_07405"
     CDS             complement(5260..6417)
                     /gene="moeB"
                     /locus_tag="VD788_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HXV76131.1"
                     /translation="MPREVDRGLALDPREIARYSRHLTLPEVGMEGQLRLRAASVLVV
                     GAGGLGSPAALYLAAAGVGRLGLVEFDRVDLSNLQRQILYGTDDVGRPKLEHAARRLA
                     EVNPHVRIDRHELRLDATNALEILRGYDLVLDGTDNFPTRYLVNDACVMLGKPNVYGS
                     IFRFEGQVSVFSTAGGPCYRCLYAEPPAPGEVPNCAEGGVLGVLPGIIGTLQATEAIK
                     LILGRGEPLVGRLLLFNALEMRFRELRLRRDAGCPVCGEHPTIRELATNAAGCATGDG
                     DGASIPEIDSPTLERRLAGERPPLLLDVRTREEWEICRIDGAFLIPLDELPRRLAELE
                     PSREIVVYCHLGGRSARAVEWLRGRGYRGAVNLEGGIDGWSIGVDPRVPRY"
     gene            6504..7301
                     /gene="thiD"
                     /locus_tag="VD788_07410"
     CDS             6504..7301
                     /gene="thiD"
                     /locus_tag="VD788_07410"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HXV76132.1"
                     /translation="MLAIAGSDSSAGAGIQADLKAIEASGGYAATVVTAVTAQNTRGV
                     RRVDRIAPDAVGAQLDALFDDLEIAAVKTGMLVGAETVCVVADVLEVRRPPIVVCDPV
                     AMSSSGAELLDETGLDALRRRIFPLATLITPNAGEAARLSGVAVDRVADAERAGRVLR
                     DAGARAVLVTGGHLAEAPATDVLVDASGTTIFAGRFVERPDTHGTGCVLASAIATGLA
                     RGLALATAIRRAKRLTATAIRRGLSIGSGARLIDALYRLHGRGTEGR"
     gene            7298..7780
                     /locus_tag="VD788_07415"
     CDS             7298..7780
                     /locus_tag="VD788_07415"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:7448..7450,aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXV76133.1"
                     /translation="MSSQEHPLRPGDTAPRFVLDGSDGERHDLDDYRGRRAVVLAWFP
                     KAYTPGUTLECKALRESGGLIRGFDVAYFAASIDGPEINRRFAASLDLDFPILSDPDK
                     SAAKAYGVLRGFGLYAARQTFFIGQDGRILHVEDEVRVRDAGAQVAARLDALGVRRRE
                     "
     gene            7785..8429
                     /gene="msrA"
                     /locus_tag="VD788_07420"
     CDS             7785..8429
                     /gene="msrA"
                     /locus_tag="VD788_07420"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXV76134.1"
                     /translation="MSSSGDKSVMVAAQHALAGRAERMRVAERHFVLGTPLDPRRGEL
                     EFAMFGMGCFWGAEKKFWEADGVYSTAVGYSAGFTPNPNYEEVCSGRTGHAEVVRVAF
                     DPANVSYESLLRVFWENHDPTQRMRQGNDLGTQYRSGIYYYDAGQHEAATQSAGAYAA
                     VLSAAGYGPVATEIRAATEFYYAEDYHQQYLAKNPGGYCGLGGTGVSCPVGIAR"
     gene            8462..12022
                     /locus_tag="VD788_07425"
     CDS             8462..12022
                     /locus_tag="VD788_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76135.1"
                     /translation="MALAWAAAPPLAAEQAAAPSRPEPRRPSVLVHCARSALAQWGWW
                     VDLEYLAELRSRGFEVDYTDLHSDLTWERLRRYDVLVTYVSPLERGRYYDNTPALPPY
                     RDEFIELVERFLDHGGGVMLMARALNADETFRPLIEPWGARIAHERIVDDDPDHLVVT
                     PRMGGRHRLWFTDGVLDGPVTAGVDQVWLPLTPHYSAFETMPIVVSDAWQVVLRGGAR
                     SRTVPIEAADERMLPFAGAYRRPKPDDPPDLFAIRSYRRGRLALCAIWPHYSIGQGTA
                     WLFDRRVLSRGVDDRPSHFGRLVENTLGWLAAGDDRDVGGFRGDAARLVAPNERPGVR
                     RAFLEREHGRDADPRRAATAATAGLRTYRGLIGAATTAGGGPARVADHAAAARRSGLD
                     FVVFLEPFEQLAPGDLERLHQACRDASGDGLQLFAGYRIETNTGNRLFVFGREAELPP
                     AELRIGERLNQQPQDPRTLAFVGGWPKLIDWIGHRLIPGRGANVGYYGLADRPQAQRA
                     TDLRLYGMLAVELYRDGERLEDNLDVYLATAAGSIPPVPVAVHLVDSPRMLERAAREG
                     IGLTHVGARSEADLWEALRYHGQDRAPNVYPSSGPEIVAWPDVFKYYTLAAEDFSGAS
                     AYMPAPLVVRADTGLHEIRIYDGERLFRRFDPRGETELSLVLELPGSIERTLTVVAED
                     ATGGRAVSTARRNWKNGDLAPVFCGDRINHCDPRPMLAKGPGSIRVLSTPEIDAGFTW
                     DGGPRGSRPLIEPERALRPRLVGELGHEGDRGFANTPRLVLADEGAVRVRSTLDTRFA
                     DEVPVINAWRTFGPLGGPSRLLRAEQHYTEFDRPTLGADPYRHPGYGRRGGASVSLFE
                     TEVTFLAGQTIERYELFVAPPTGDVAVVHVVHGRGEREIASYRLSDGDVEPRGARLER
                     GDWIAFYADGGFNTAVQFNRGEPLDVELDPHRGVVYRHAGGPFAVREGERRRFELLSA
                     VDPLDHVPGGAERAREIVRFLLDPLGDGPGGPAPSAGVIELDARDGTASIDLERSSRV
                     PGVPLPVVVSGLNPNWSAGVLLERGYVLGGYGSGADRYRAAAVDPAGRLYLPLFPDLA
                     PRTSVVAGHPVVCDRRELFIQVTLARDRPAPGAWHVSINNPLHQPVRTTVRVVMPMVG
                     LAAGEREVVVPAGGYLVLEPVDFSRTSGSTASTDPDGRRR"
     gene            12100..12699
                     /locus_tag="VD788_07430"
     CDS             12100..12699
                     /locus_tag="VD788_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76136.1"
                     /translation="MKEVLPGVHHWTALHPKIKIAVSSYYLADERVLIDPLWPDAGFE
                     PSGAGPTDILLTNRHHYRDSRGFVERYGCTVWCAESGMHEFTHGERVRPLGPGDRLPA
                     GITAHAIGAICPDETAFSIARGAGLLALGDGVVRRDDGPLGFVPDAYMGDDPESVKDG
                     LRRAFAALGGLRFDHLLLAHGWPWIGGGKAALLGFSRAG"
     gene            12787..14226
                     /locus_tag="VD788_07435"
     CDS             12787..14226
                     /locus_tag="VD788_07435"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HXV76137.1"
                     /translation="MRWIRQIVRAAAVAATLAASGSPVFAGIEATYLANEGFFLTGGG
                     KKVLIDASFDAGIAPYAVLADTTRHAIATGAPPFDDVDLLLATHVHADHFAPEVVLSH
                     LRANPRARFVSTPDAIAALEPLLGGDASLAERITAVYPDEGRPETLERSGVPLRVINL
                     HHGRGGERPVQNLGFVVTLGDTRLLHVGDTVASADELGAAAIGPVDVAFLPYWLLIGA
                     AEARVVGADRIVAMHLPPAGAPAGHFDPYASFEDAAERIARLHPGAVVPARQGERRVL
                     ERPKPRSHAPRRAAPPTAWVTARTAAGPGRFLVAHREMLDPNFAETVVLLVDHDANGA
                     LGLVINRPSELTLSALSSEIEGLRGRDDPVYVGGPVPGGPIFVLIRDDAVAGDAKRVI
                     DGVYLSRDQAILEHLTGRPADEFRVYAGYAGWAPGQLDHELERGDWHVVPAAADWVFD
                     PRPAEVWRRLVPPEPSRQAMSWAGAAGAD"
     gene            complement(14223..15017)
                     /locus_tag="VD788_07440"
     CDS             complement(14223..15017)
                     /locus_tag="VD788_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76138.1"
                     /translation="MKRIPVFATFFLCSSLVVLPAIAGEAAALSLDVLVDGLPVAQHA
                     ARGRSYVEAIEGREYSIRVTNHEPRRVAVALSVDGLNTIDAKSTSAREASKWILDPGE
                     SITIDGWQTSGSTARRFYFTSEDRSYGAWLGRRENLGLISAAVFRERPRPSRPIVVPL
                     EPGRSSGPSARSEAAPRSVESEDRGLAATGIGREVDHRVRRVRFDAEPDPAARVELRY
                     EYRSELVRLGVLPAPCPGPDDPLARRERSRGFDDGEFAPDPFRADR"
     gene            complement(15118..15771)
                     /locus_tag="VD788_07445"
     CDS             complement(15118..15771)
                     /locus_tag="VD788_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXV76139.1"
                     /translation="MGEWVLYLGNRNYSSWSMRAWLAMKQAGFEFDEVRFHLGQPDVR
                     QRIAQVSPTGRVPALHHRGRAIWDSLAIGEYLAERAPDAGLWPRAAEPRAVARSVVAE
                     MHAGFPGLRSRMPFNVRRSSPGVGREAEVEAEIDRVFAIWRTCREDFGGDGEFLFGAF
                     GLADAFYAPVVSRFKTYAVTIERDCRAYVDAVWQQPWVRQWRAEAEAEEWVEPRFDL"
     gene            15935..17902
                     /locus_tag="VD788_07450"
     CDS             15935..17902
                     /locus_tag="VD788_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HXV76140.1"
                     /translation="MNIGVIGSGSIGPDLAYGFVSALSRAGGGRVYLHDIRREALDAG
                     LERIRGYVGKGVSRGKISAKAAREIEQTLIPTLELADLAGCDYVLEAATEDLETKRKI
                     LARLEQTVRADCLIGFATSGIPRARIAAGAVHPERCFVNHPFFPAWRSLPVEVVLSGD
                     ARTGERMLDTLTMLGKIPVVTADVPCFAADDIFCNYCAEAARIVAEGVATPAQVDAIV
                     HQAIGGGGPFNVMDLTQGNLLNVHCLELMQHGPDGSDWFAPPAIFTRQGHKPWIDRKN
                     PGDPTHDAALAERVLDRILAVLLGRTYYVVDHEICNPTELNWLTRTALGFRDGLLDLA
                     ASLGADRVREICTSYAASHPGYVVPDSIAERRPLAFHGNVRLDHDGEIAVVTVFRPEV
                     KNALDERTMDELARTFAALAEDDAIRGVVLTSPDGALAGADITELAALATPQEAADKC
                     RRGQAVLDRIATFAKPVVAAVDGPVLGGGAELSMACHARVVGRSLMLGQPEVNLGIIP
                     GYGGTQRLPRLVGVERGQELLRTGRPVGPREACEWGWAHGEPAGKPVAVAKELVRRHL
                     AGEVQLAAVSAAPMDLPERLAPVALGHRSLAVDAILVDTLRRGLTRPLPDGLGVEAEN
                     FARCQQTVDYDIGMKNFMQNGPRVPAVFINE"
     gene            17911..19194
                     /locus_tag="VD788_07455"
     CDS             17911..19194
                     /locus_tag="VD788_07455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXV76141.1"
                     /translation="MMASNESIVILDGARRTPRADILIDNKEPGLFSRFTATQLGGMA
                     IAATLEHTPIDPALIGHVVMGMAQHANRDSIYGAQGMRWRGGLGQDVPALTVARICGS
                     GAEAVAVGCEMILAGVRHDRKRPFSVVGGAESMQYPFCLFNYRGRPVGEAVQKYGPID
                     VKALPPGTHLQDALLMGLYDPGARMAMANTAEELGRRYEITRVEADEFGFRSHRLAKQ
                     AREAGWFDEEIEPVRVEPDGSSEPIEVRYDTHILDDVSLEKMARLRPAFEPGGIITAG
                     NASAVVDGAAAMVIGKQSDAEEHGLRPLARVLGFGVAACDPRIMGWGPVPAARRAFES
                     SGIDAAQIDLVELNEAFAPQALAVIRDFERMGIDPQKVNPMGNAIALGHPLGATGSIL
                     TLTCAYALRRTGQRYGMVTMCIGGGQGIALILESM"
     gene            19208..>19423
                     /locus_tag="VD788_07460"
     CDS             19208..>19423
                     /locus_tag="VD788_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76142.1"
                     /translation="MGPIDERRALDHLIELLSVEGPSGGEHHVSQTIRARARAAGCPA
                     ASIIEDDAHLRIGDGFRTGNLIIRMPGT"
BASE COUNT         2700 a         6803 c         7061 g         2859 t
ORIGIN      
        1 gagggggcgc ggttgtaccg agaccagaaa gtgatcgtcg tgatgccggc ctacaacgcc
       61 gcgcggacgc tctatacgac gtacgccgag gtgatggcgc aggactacgt cgacgcggtg
      121 atcgtggtcg acgacgccag caaggacgcg acggtcgaca tcgcgcaggg tctggcgcgg
      181 gtcacggtcc accgccacga tgccaatcgc ggctacggcg ccaaccagaa gacctgctac
      241 cggttggcgc tcgatgccgg ggccgacatc gtcatcatgg tgcacccgga ctaccagtac
      301 acgccgaagc tgatcccggc gatgacatcg atgatcggca acggtctgta ccactgcgtc
      361 atcgcgtcgc gcatcctcgg cggctatgcg ctgcgcggcg gcatgccgtg gtggcgctac
      421 gtcagcaacc gcgtgatcac gttctacgag aacgcgttga tcggcgcgaa gctgtccgag
      481 tatcacaccg gctatcgcgc gttctcgcga cggctgctcg aggcgttgcc gctcgaggcg
      541 aactccgacg acttcgtctt cgacaaccaa atgctggctc agatcctgtg gatggggttc
      601 gtcgtcgccg aggtcagctg ccccacgcgt taccacgccg aggcatcgtc gatcgacttc
      661 cgtcgcagcg tgcgatacgg cttcggctgc ctgtggaccg cgacgcagtt cttcctggcc
      721 aagcacgact gggccggcct caggcgtttt ccacgcgagc tgcgcgagac cggcgcgcgg
      781 ggttgagcgg tttgaccggg acccggcggt cgatcggttt cgcgccgtct ctggtcgtca
      841 tcctgctcat cggcgccgtg ctgcgcgccg gctacaccat cgcgcaggcc cgctccgatc
      901 cgtggttcgc gctgccgatg ctcgacgggc gctactacgt cgagtgggcg gcatcgctcg
      961 cggccgggcg cggcggtccg cccggggcgt tctacctggc gccgctctac ccctacctgc
     1021 tggcgttgct gatccggctg ggcgggctgt cctttctcgg cctgtacgtg gtgcagcagg
     1081 cgatggggtt ggcgtcggcg gcgattctcg ccgtcggcgg ccgccgcgtg ctgggcgaga
     1141 cggcggcgct cgcgaccgcg ctgctgttcg tgctccacca tccgctgcaa ttcttcgcgg
     1201 cacgtccgct cggcgaggcg ctcgcgatct tgctgctgac ggcggcgttg gcgctggtct
     1261 ggcgcacggg tccgcgggcc ggtctggccg ccggggtctg tctcggcctg gcggccctca
     1321 cccgacccaa cctgctgctg gtgctgccgg cctgggtcgt cgtcgagtcg cgcgatcgac
     1381 gctggcgtcg cctggcgacc ctcgtcctcg gcttcgccgt cgtcgtggcg ccggtcacgc
     1441 tgcggaatcg gatcgtctcc gggcactggg tcgcgatctc atcgaacggc ggcctgaccg
     1501 cctaccacgg caacggcccg ggcgcgcgcg gggtctacac ccaccccgcc gggttcccgt
     1561 tcgagatgca tcagcaacgg gaggcggcca cgcggcgggc ccgccagctc agcgggctcg
     1621 cgctcgatcc ggtcgaggcc gacgcctggt ggggtcgcaa ggcgctggag gcgcggctcg
     1681 cacgaccgct cgacacggcc ggtctcgtgg cctggcgggt cgcgctgacg ctcgacaacc
     1741 gcgagctgag cctcgactac ccgccgatgc tcgaccccaa cccgtggcgc accacgctgc
     1801 ggctgccccc gggccacgag ctggcctggg tgccgtgggc gcttctggtc ggtctggccg
     1861 gtgcgggtgc ggtcggtgcc ggctggcgcc gcagcggagg ggcgcacgcc tggggcgcgc
     1921 tgatcgccgc cctggcaacc cccgtgctgt tctacgtgtc gagccgctac cggttgccgg
     1981 ctgcggccct gctcgcggtg ccggccggtg cgggtgtggc cgtgctgctc gaacgctccg
     2041 tcggtgcgcg gcgctggcgc gcgctcgcga tggcggtcgc gctgatcgcg ctgtcgatgc
     2101 tcgtgccgtc cgccgcgctc gaacggacgc agcgtgccga ggggctcggc aaccgcgccg
     2161 cggcctacct ggcgtgcggt cagctcgagc gggccgaggc cgacgcgacc cgcgccgtcg
     2221 agctcgaacc ggacgcgccc cggctgtggg ccaccctcgg cgcggtgatg gatcggcgcg
     2281 agcggttcgc cgccgcagag caggcctacg agcgcgcgct cgcgctcgag cccattccgg
     2341 aggccgccgc cggtctggcc gcggcgctgg ccgcgcaggg acgggtcgat cggggggttg
     2401 ccgtgctgcg ccgcgcgctc gtgctcgcgc ccggcaacgc cgcctgctgg aacggactgg
     2461 tgggcctgct gtacgcttcg ggcgacctcg cggccgcgcg ccggaccgcg cgggaagcgg
     2521 tcgccgccgg ggtggagctc gacggcgagc tgcgctcact cgccgagggt ggagcggagg
     2581 atggctcgta ggtcgacccg tggcatcgtc ggactcgccg cgtcggcggt cctcaccggc
     2641 gcgctcggtt gctccccgac ggtgacggac accggtttcg ccggcacgtg ggccagcgag
     2701 gtgcaggtcg gaggccgctc ggtcgtcgcg atctggcgcg acggcagcgg ttaccgtttc
     2761 gcggtcaacc ggtacgatgc cgacggccgg caccatctgc gctgtacggc cgagggcgag
     2821 tgcgcgtatt tcgacggtga agcgccgtac tacgcgctgg agttcgccgt gcgggccgga
     2881 tcgaacgacg gcgcgctcta cgtcgactgc aaggggcatc cgctcgaggg taaggagggc
     2941 acgccgatcg aatgggtcga gcggttgtcc gtcgagccgg gcggcatcgt gctgacggca
     3001 cagcgcatcg agctgaacgg tacgccggtc acggccatgc cgcggcgttt cgccaagcag
     3061 tcggaccgac cgcgcttccg atgagccggc catcgatcga ccgcacggcg ggcgggctcg
     3121 agcgggctct cggctatcgc ttccgggacc gcacgctgct cgagcgcgcg ttgacgcacc
     3181 gctcgcacgt ccacgagcgg gagggggaga ccggcgaaag ctacgagcgt ctcgagttcc
     3241 tcggtgacgc gctgctcggc atgttcgtcg ccgattggct ctaccgtaaa gatccgcggg
     3301 ccggtgaagg cacgctcagc cgcggtcggc agacggtggt ccgcgcctcg tcgctggcgc
     3361 gcatcgcgtc cgcgctcggc ctgggcgacg cgatcctgtt gggacgcggc gaggagctga
     3421 ccggtgggcg actgaagcgt tccctgctcg ccgacgtctt cgaggcggtg gtcgccgcga
     3481 tctacctcga tggcggtctg cggcaagcgc gcgccttcgt ccaccgtcat ctcggccccg
     3541 agctaggccg cgccgtcggg ccgagcccgg cgtcggacga cttcaagacc cgcttgcagg
     3601 aggtggtgca gggcaccttg cagcgtacac cgagctaccg tatcgtttct acggccggcc
     3661 cggcgcatgc gctcagcttc gtcgtcgagg tgctcgtcgg tgacgaggtg ctcggccgcg
     3721 gacgcggttc gaaccgcaag cgggccgagc aggaggcggc caggtgcgcg ctcgatcgat
     3781 tcgagtcgac cgaccgatga agggcgattc ccgtaatccc gtccccgtga cacggctgtg
     3841 cggcatgctc gcgctgtgcg ggctgtcggg cgcggtgtcg gccgaggagc cggtgctgct
     3901 caacgaccgg tcggggggcc agatcgtcat ccaagcggag accgagctga gcatcgaagg
     3961 cttttcgggg accgtcgccg tccgtgcggg caagagcggc gaactgcgct acgagatccg
     4021 tgcgctcgcc gaccgcaacc agccgcgcgc gctggcgttg tggtccacga agggcgtgtt
     4081 gcgactggtt ccgatcgaac caccggccac cgaaccgctg ctggtcgaga tcgccgtccc
     4141 gcccgcgctg gccgtcaacg tcgacttgtc ggattccaag gtcgaggtca gcggcctgaa
     4201 cagcccggtc ggcgtgaccg gagcccggct ggagctcgac atccgcggga tcgccggtac
     4261 cgtcgacctc gccgtcagcg aggcgacggt gcggatcgac ggcatcgcgg gcgacgtgtc
     4321 gttcgagggt gagtcggtgc ggctcgacct gcgccgcgtc cagggctcag ccggactgac
     4381 cgtccagggc ggcgaagtga cgttggccga ggtcagcggc gagaccgacg cggacctggg
     4441 cgagacggtg ttcaacgccg tcgcgttggt ccacggcgcg cgtgttcagg cggccggtgg
     4501 gcggatcagc ctcggccagg tcagccagca ggtcgagctg gtcgtcgagg ggaccccggt
     4561 cgagatcagc ggcgccagcg ggcgggtgac ggtcgattcg gactcggagg tcacgctcaa
     4621 cgaggtccaa ggcacggtga tcctcaccgg ctacggcggc gtgatccgcg ggacggcgag
     4681 cagcggtccg ctcgagatca cgatcgacga cgccgatgtc acgctcgagc agtggcaggg
     4741 caagatcgac gttcgcggcg acggtctcga tctcgcgatg cgacagatca cgggatcgat
     4801 cgccgcggcg acgacgtcgt cgaacttcgt gatcgagagg gtcgaggggc cggtcacgat
     4861 cgagaacgag ttcggtgacg tgcgggtctc cggcgtgagc ggcacgacca agatcgtcaa
     4921 tcgcgacggc atcaccgtcg cggccgacct caacggccaa ctcgacctgc acgggagcgg
     4981 cgaacgcgtc gaggtgtctt gggcccagct cggctccgat gcgaaccacg tgatcgagaa
     5041 cgcggcgggt caagtcgtgc tgcgcgtccc gacgaacgcg cgctgccggt tcgacgtgcg
     5101 gtcggaattc ggcgaggtga actcgcagct gcccggcgtc caggtcagcg acgacggcac
     5161 cttcgcctcg ggcgcgctcg gcggggcgga gcaaccgacg atccaggtcc gctccagcgg
     5221 agacgtcgag ctgtcgcagt acagcccgcc ggcgccgact cagtagcgcg ggacgcgcgg
     5281 gtcgaccccg atcgaccagc cgtcgatgcc gccttcgagg ttgaccgcgc cgcggtagcc
     5341 gcgcccccgc aaccattcga ccgcgcgcgc gctgcggccg ccgaggtggc agtagacgac
     5401 gatctcgcgg gacggttcga gctcggccag gcgccgaggc agctcgtcga gcgggatcaa
     5461 gaacgcgccg tcgatccggc agatctccca ttcctcgcgc gtgcgcacgt cgagcaacag
     5521 cggcggccgt tcgccggcca gtcgtcgttc gagcgtcggc gagtcgatct cggggatcga
     5581 cgcgccgtcg ccgtcgcccg tcgcgcagcc ggcggcgttc gtcgccaact cgcggatcgt
     5641 cgggtgctcg ccgcagaccg gacagccggc gtcgcgccgc agccgcagct cgcggaagcg
     5701 catctcgagc gcgttgaaca gcagcagtcg cccgaccagc ggctcgccgc gaccgaggat
     5761 cagcttgatc gcctcggtcg cctgcagcgt gccgatgatc cccggaagta cgccgagcac
     5821 cccgccctcg gcgcagttgg gcacctcacc cggcgcggga ggctcggcgt agaggcagcg
     5881 gtagcagggg ccgccggccg tcgaaaagac cgaaacctgc ccttcgaagc ggaagatgct
     5941 gccgtagacg ttcggcttgc cgagcatcac gcaggcgtcg ttgaccaggt agcgcgtcgg
     6001 gaagttgtcg gtgccgtcga gcaccaggtc gtagccgcgg aggatctcga gtgcgttcgt
     6061 cgcatcgagc cgtagctcgt gtcgatcgat gcgcacgtgc gggttgacct ccgccagacg
     6121 ccgcgcggcg tgctcgagct tcggtcgccc gacgtcgtcc gtgccgtaga ggatttggcg
     6181 ctgcaggttg ctcaggtcga cgcggtcgaa ctcgacgagc ccgagtcgcc cgacgccggc
     6241 cgcggccagg tacagcgcgg cgggcgatcc gagcccgccg gcgccgacga cgagcacgct
     6301 tgccgcgcgc agacggagct ggccttccat gcccacctcg ggcagggtaa ggtgtcggct
     6361 gtaacgggcg atctcgcgcg ggtcgagtgc gagcccccga tcgacttctc gcggcatcgc
     6421 gcgctcctgt cgcggaggag tattgtgcac ggcgcggcgg cttggttgca atggacctgt
     6481 accggacagc ggtaagaccg gtcgtgctgg ccatcgccgg ttcggacagc tcggccggag
     6541 ccgggatcca ggccgacctc aaggcgatcg aggccagcgg cggctacgcg gcgaccgtgg
     6601 tgaccgcggt cacggcccag aacacccgcg gcgtacgccg cgtcgatcgg atcgcccccg
     6661 acgcggtggg cgcgcagctc gacgcgctgt tcgacgacct ggagatcgcc gcggtcaaga
     6721 ccgggatgct ggtcggcgcc gagacggtgt gcgtggtggc cgacgtgctc gaggtgcggc
     6781 gtccgccgat cgtcgtctgc gacccggtgg cgatgtcgag cagcggggcg gagctgctgg
     6841 acgagacggg actcgacgcc ctgcggcgcc ggatcttccc actcgccacg ctgatcacgc
     6901 ccaacgccgg cgaggcggcg cggctcagcg gagtcgcggt cgatcgggtg gccgacgccg
     6961 aacgggcggg tcgcgtgctg cgcgacgccg gcgcgcgcgc ggtgctggtg acgggcggcc
     7021 acctggccga ggcgcctgcg accgacgtgc tggtcgacgc gtcgggcacg acgatcttcg
     7081 ccgggcgctt cgtcgagcgc cccgacacgc acggcacggg ctgcgtgctg gcgtcggcga
     7141 tcgccaccgg cctcgcccgc ggcctcgcgc tcgccacggc gatccgccgg gccaagcggc
     7201 tgaccgcgac ggcgatccgg cgcgggctgt cgatcggctc gggtgctagg ctgatcgatg
     7261 cgctctaccg cctgcacggc cgaggcacgg aaggacgatg agcagccagg agcatccctt
     7321 gcggccggga gacaccgcgc ctcgcttcgt gctcgacggc tcggacggcg agcgccacga
     7381 cctcgacgac tatcgcggcc gacgggccgt cgtcctggcg tggtttccca aggcctacac
     7441 accggggtga acgctcgagt gcaaggcgct ccgggagagc ggcggtctga tccgcgggtt
     7501 cgacgtggcc tacttcgccg ccagcatcga cgggccggag atcaaccgca ggttcgccgc
     7561 ctcgctcgat ctcgactttc cgatcctcag cgatccggac aagagcgcgg ccaaggcgta
     7621 cggcgtgctg cgcggtttcg gcttgtacgc ggcgcgccag acgttcttca tcggacagga
     7681 cgggcggatc ctgcacgtcg aggacgaagt acgcgtgcgc gacgcgggag cccaggtcgc
     7741 cgcgcggctg gacgccctcg gcgtgcgacg gagggagtga aacgatgtcg tcgagcgggg
     7801 acaagtcggt gatggtcgca gcgcagcacg cgctcgccgg acgggcggaa cggatgcgcg
     7861 tcgccgaacg acacttcgtg ctcggcacgc cgctcgatcc gcgccgcggc gagctcgagt
     7921 tcgcgatgtt cggcatgggc tgcttctggg gtgcggagaa gaaattctgg gaagccgacg
     7981 gggtctactc gaccgcggtc ggctattcgg ccggcttcac gcccaacccg aactacgagg
     8041 aggtctgcag cgggcggacg ggtcacgcgg aggtggtgcg cgtggcgttc gacccggcca
     8101 acgtctcgta cgagtcgttg ctgcgtgtct tctgggagaa ccacgacccg acacagcgga
     8161 tgcggcaggg caacgacctc ggcacgcagt accgttccgg catctactac tacgacgccg
     8221 gccaacacga ggcggcgacg caaagcgccg gggcctacgc ggccgtgctg tcggctgccg
     8281 gctacggtcc cgtcgcgacc gagatccgcg cggcgaccga gttctactac gccgaggact
     8341 accaccagca gtacctggca aaaaaccccg gcggctattg cggtctgggc ggcaccggcg
     8401 tgagctgccc ggtcgggatc gcccgctgat tcgtcaccgc cctcgagcgc ggtggctcgt
     8461 cgtggcgctc gcgtgggccg cggcgccgcc gctcgcggcc gagcaggccg cggctccgtc
     8521 gcggcccgag ccgcgacgtc cgtcggtcct cgttcactgc gcgcgatccg cgctcgcgca
     8581 gtggggttgg tgggtcgacc tcgagtacct ggccgagttg cgatcgcgcg ggttcgaggt
     8641 cgactacacc gatctccact ccgacctgac ctgggagcgg ctgcgacggt acgacgtgct
     8701 cgtgacctac gtcagtccgc tcgagcgcgg gcggtactac gacaacacgc cggcactccc
     8761 gccgtaccgc gacgaattca tcgagctggt cgagcgcttc ctcgatcacg gcggtggcgt
     8821 gatgctgatg gcccgcgcgc tcaacgcgga cgagacgttc cgaccgctga tcgagccgtg
     8881 gggcgcccgc atcgcgcacg agcggatcgt cgacgacgac cccgaccatc tcgtcgtcac
     8941 gccgcggatg ggcggccggc accggctgtg gttcaccgac ggcgtactgg acggcccggt
     9001 gacggcgggc gtcgaccagg tgtggctgcc gttgaccccg cactactccg cgttcgagac
     9061 gatgccgatc gtggtctcgg acgcatggca ggtcgtgtta cgcggcggcg cgcgctcgcg
     9121 gaccgtgccg atcgaggcgg cggacgagag gatgctgccg ttcgccggcg cctaccggcg
     9181 cccgaagccc gacgatccgc cggacctgtt cgcgatccgg agctaccgcc gtgggcgtct
     9241 cgcattgtgc gcgatctggc cccactattc gatcgggcag ggcacggcgt ggctgttcga
     9301 ccggcgggtg ctgagccgcg gggtcgacga tcgcccgtcc cacttcggtc ggctggtcga
     9361 gaacacgctc ggctggctcg ccgccggcga cgatcgcgac gtcggaggct ttcgcggcga
     9421 cgccgcgcgg ctcgtcgctc ccaacgagcg ccccggcgtc cgccgagcgt tcctcgagcg
     9481 cgagcacggc cgcgatgccg atccgcggcg cgcggcgacc gcggccaccg ccggcctgcg
     9541 cacctaccgc ggtctgatcg gggccgcgac gacggccggc gggggtccgg cgcgtgtcgc
     9601 ggatcacgcc gccgcggctc gccgttccgg tctcgacttc gtcgtcttcc tcgagccgtt
     9661 cgagcagctc gcgcccggcg acctcgagcg gctgcaccag gcctgtcgcg acgcgtcggg
     9721 cgacgggctg caactgttcg ccggctaccg gatcgagacc aacaccggca accgcttgtt
     9781 cgtcttcggc cgcgaagccg agctcccgcc ggccgagctg cggatcggcg agcggttgaa
     9841 ccagcagccg caggacccgc ggacgttggc cttcgtcgga ggctggccga agctgatcga
     9901 ctggatcggc caccggctga tccccggccg cggcgcgaac gtgggctact acggtctcgc
     9961 cgatcggccg caggcgcaac gcgccaccga tcttcgtctg tacggcatgc tcgccgtcga
    10021 gctgtaccgc gacggcgagc ggctcgaaga caacctcgac gtctatctcg ccaccgcggc
    10081 gggctcgatt ccgccggtcc cggtcgccgt ccacctcgtc gactcgccac ggatgctcga
    10141 gcgcgcggcg cgggaaggga tcgggctgac ccacgtcggc gcgcggtcgg aggccgatct
    10201 gtgggaggcg ttgcgttatc acggacagga ccgggcaccg aacgtctacc cgtcgagcgg
    10261 gcccgagatc gtcgcgtggc ccgacgtgtt caagtactac acgctggccg ccgaggattt
    10321 ctcgggcgcc tcggcctaca tgccggcgcc gctggtcgtg cgcgccgaca ccggcttgca
    10381 cgagatccgc atctacgacg gcgagcgcct gttccgccgc ttcgatcccc gcggcgagac
    10441 cgagctgtcg ctcgtgctcg agctgccggg ctcgatcgag cgcacgctga ccgtcgtcgc
    10501 cgaggacgcc accggcggtc gtgccgtcag caccgcccga cgcaactgga agaacggcga
    10561 tctcgcgccg gtcttctgcg gcgaccggat caatcactgc gacccgcggc cgatgctggc
    10621 caagggtccg gggagcatcc gcgtcctgtc gactcccgag atcgacgccg gcttcacctg
    10681 ggacggcggg ccacgcggat cacgcccgct gatcgagccc gaacgcgcgc tgcgcccgcg
    10741 gctggtcggt gagctgggcc acgagggcga ccgcggcttt gccaacacgc cccgcctggt
    10801 gctcgccgac gagggcgcgg tgcgggtgcg atcgacgctc gacacgcgct tcgccgacga
    10861 ggtgccggtg atcaacgcgt ggcgcacgtt cggaccgctc ggcggcccat cgcgcctgct
    10921 gcgcgccgag caacactaca ccgagttcga ccggccgaca ctcggtgccg acccctaccg
    10981 ccacccgggc tacgggcggc gcggcggtgc ctccgtctcg ttgttcgaga ccgaggtgac
    11041 cttcctcgcc ggacagacga tcgagcgcta cgagttgttc gtcgcaccgc cgactggcga
    11101 cgtcgcggtc gtgcacgtcg tgcacggtcg cggcgagcgt gagatcgcga gctaccgctt
    11161 gtcggacggc gacgtcgagc cgcgcggcgc tcgactcgag aggggcgact ggatcgcgtt
    11221 ctacgccgac ggcgggttca acacggccgt ccagttcaat cgcggcgagc cgctcgacgt
    11281 cgagctcgat ccccaccgcg gcgtggtcta ccgtcacgcc ggagggccgt tcgcggttcg
    11341 cgaaggcgag cggcgccgct tcgagctgct gagcgccgtc gatccgctcg accacgtgcc
    11401 gggcggcgcc gagcgcgcgc gggagatcgt gcggttcctg ctcgatccgc tcggcgatgg
    11461 ccccggcggg ccggcgccgt cggccggcgt aatcgagctc gatgcgcggg acggtacggc
    11521 atcgatcgat ctcgagcgct cgtcgcgggt gccgggggtg ccgctgccgg tcgtggtgtc
    11581 gggtctcaac ccgaactgga gcgcgggggt cctgctcgag cgtggctacg tgctcggtgg
    11641 ctacggctcc ggggccgatc gctaccgggc ggccgcggtc gatcccgccg gccggctcta
    11701 tctgccgttg ttccccgatc tcgccccgcg tacgagcgtc gtcgccggcc acccggtcgt
    11761 ctgcgaccgg cgcgagctgt tcatccaggt cacgctggcg cgcgaccggc cggcgccggg
    11821 cgcctggcac gtctcgatca acaacccgct ccatcagccg gtccgcacca cggtgcgcgt
    11881 ggtgatgccg atggtcggcc tggcggccgg cgagcgcgag gtggtcgtgc cggccggcgg
    11941 ctacctcgtc ctggaaccgg tcgatttcag tcgaaccagc ggatcaaccg cgtcaacgga
    12001 ccccgacggg cgtaggcggt gacgtggtcg cccgcttcga gccgcgtgct gccgcatgaa
    12061 tagacgacgt cgccgtcgct gcgaccaagg aggatcacga tgaaggaagt gcttccgggc
    12121 gtccaccact ggaccgcgct gcatccgaag atcaagatcg cggtcagctc gtactacttg
    12181 gcggacgagc gcgtactgat cgacccgctg tggcccgacg cgggcttcga gccgtcaggc
    12241 gccgggccga cggacatcct gctcaccaac cggcaccact atcgcgacag ccggggcttc
    12301 gtcgagcgct acggctgtac ggtgtggtgt gccgagtcgg gcatgcacga gttcacgcac
    12361 ggcgagcggg tgcggcccct cgggcccgga gaccggctgc cggccggcat cacggcacac
    12421 gcgatcggtg cgatctgccc cgacgagacg gcgttttcga tcgcgcgcgg cgccgggctg
    12481 ctcgcactcg gcgacggcgt ggtgcgtcgc gacgacggac cgctcggctt cgttcccgac
    12541 gcctacatgg gcgacgaccc cgagtcggtc aaggacgggc tgaggcgggc gttcgccgcc
    12601 ctcggcggtt tgcgcttcga ccatctgttg ctggcccacg gctggccgtg gatcggtggc
    12661 ggcaaggcgg cgttgctcgg attctcgcga gcgggctgag cggagggtcc gctcgccggg
    12721 ccgcggggtc gaaccacgcg gcacaccgcg cgtataatcg tgggcggcct ggggcgcggg
    12781 ggctcgatgc gttggatccg tcagatcgtc cgggcggcag cggtcgcggc gaccctggcc
    12841 gcgagcgggt cgcccgtctt cgccgggatc gaggcgacct acctggccaa cgaaggtttc
    12901 ttcctgacag gcggcggcaa gaaggtgctg atcgacgcct cgttcgacgc cgggatcgcg
    12961 ccgtacgccg tgctcgccga tacgacgcgc cacgcgatcg cgacgggcgc tccaccgttc
    13021 gacgacgtcg acctgctgct cgcgacgcac gtccacgccg atcacttcgc ccccgaggtc
    13081 gtgctgagcc acctgcgggc caacccgcga gcccgattcg tctcgacgcc cgacgcgatc
    13141 gccgcgctcg agccgctgct ggggggcgat gcgtcgctgg ccgagcggat caccgcggtc
    13201 tatcccgacg aggggcggcc cgagacactc gagcgatcgg gcgtgccgct gcgcgtgatc
    13261 aacctgcacc acgggcgggg cggcgagcga ccggtacaga acctgggttt cgtcgtcacg
    13321 ctcggcgata cgcgcctgct ccacgtcggt gacaccgtcg cctcggccga tgaactcggc
    13381 gcggcggcga tcggcccggt ggacgtcgcg ttcctgccgt actggctgtt gatcggtgcg
    13441 gccgaggccc gagtggtggg ggccgaccgg atcgtggcga tgcacctccc tcccgccggc
    13501 gcaccggcgg gtcacttcga tccgtacgcg agcttcgagg acgccgccga gcgcatcgcc
    13561 cgtctgcacc ccggtgcggt cgtgcccgcg cggcagggtg aaagacgcgt gctcgagcgg
    13621 ccgaagccac ggagccacgc cccgcgtcgt gccgcaccgc cgaccgcgtg ggtcacggcc
    13681 cggaccgcag ccggccccgg gcggttcctc gtcgcccacc gcgagatgct cgaccccaac
    13741 ttcgccgaga cggtggtgct gctcgtcgac cacgatgcga acggcgcact cggcctcgtc
    13801 atcaaccggc cgagcgagct gacgctgtcg gcgctgtcat cggagatcga agggctgcgc
    13861 ggccgggacg acccggtcta cgtcggcggg ccggtccccg gcggaccgat cttcgtcctg
    13921 atccgggacg acgccgtggc gggcgatgcc aagcgcgtca tcgacggcgt ctacctctcc
    13981 cgcgaccagg cgatcctcga gcatctcacc gggcggccgg ccgacgagtt ccgcgtctac
    14041 gcgggctacg ccggttgggc gccgggccag ctcgatcacg agctcgagcg aggtgactgg
    14101 cacgtggtgc cggccgcggc ggactgggtt ttcgacccgc ggccggccga ggtgtggcgc
    14161 cgactcgtgc cgcccgagcc cagccgccag gcgatgtcgt gggccggcgc ggcgggtgcc
    14221 gactaacgat cggcgcggaa cggatcgggg gcgaactcgc cgtcgtcgaa tccgcgggag
    14281 cgctcgcgcc gggccaacgg gtcgtccgga ccggggcacg gcgcgggcag cacgccgagg
    14341 cggaccagtt ccgaccggta ctcgtagcgc agctcgacac gcgcggcggg gtcgggctcc
    14401 gcgtcgaagc gcacgcggcg aacccggtgg tcgacctcgc ggccgattcc ggtggcggcg
    14461 aggccgcgat cctcgctttc gacactccgc ggggccgcct cggaccgcgc cgacggtccg
    14521 ctcgaccgac ccggttcgag cggtaccacg atcggtcgcg acggccgcgg ccgctcgcga
    14581 aagacggcgg ccgagatcaa gccgaggttc tcccggcgcc cgagccacgc gccgtaggag
    14641 cggtcctcgc tggtgaagta gaagcgacgc gcggtcgacc cgctcgtctg ccagccgtcg
    14701 atcgtgatcg actcccccgg gtcgaggatc cacttcgacg cctcccgcgc cgacgtcgat
    14761 ttggcgtcga tcgtgttcag gccgtcgacg ctgagtgcga ccgcgacgcg gcgcggctcg
    14821 tggttcgtca cgcgaatcga gtactcccgg ccctcgatcg cttcgacgta actccggccg
    14881 cgcgcggcgt gttgggcgac cggtaggccg tcgacgagca cgtcgagcga gagcgccgcc
    14941 gcctcgccgg cgatggccgg caggacgacg agcgacgagc aaaggaagaa agtggcgaag
    15001 accgggatcc tcttcatcgt tcggctcctt tcgtggctgc cgggacagac acctcggggg
    15061 cggaaaggtt cccggcgcgg cgttccgccg tgccgcctca acccccgccg gcccagctca
    15121 caggtcgaac cgcggctcga cccactcctc ggcctcggcc tccgcgcgcc actgccgcac
    15181 ccacggctgc tgccagacgg cgtcgacgta ggcccggcag tcgcgttcga tcgtgaccgc
    15241 gtacgtcttg aatcgactga cgaccggggc atagaacgcg tcggccaggc cgaacgcgcc
    15301 gaacaggaac tccccgtcgc cgccgaagtc ctcgcggcag gtgcgccaga tcgcgaagac
    15361 gcgatcgatc tcggcctcga cctccgcctc gcggcccacg cccggactcg atcgtcgcac
    15421 gttgaacggc atccggctcc gcaggcccgg aaagcccgcg tgcatctcgg ccacgaccga
    15481 gcgggcgacg gcgcgcggtt cggccgcgcg cggccacagg ccggcgtcgg gcgcccgctc
    15541 ggccaggtat tcgccgatcg ccagcgaatc ccagatcgcg cgtcctcggt ggtgcagcgc
    15601 cggcacgcga ccggtggggg agacctgcgc gatccgttgc cggacgtcgg gctggccgag
    15661 atggaagcgg acttcgtcga actcgaagcc cgcctgcttc atcgcgagcc acgcgcgcat
    15721 cgaccaggag gaataattcc ggtttcccag gtagagtacc cactcaccca ttctcggctg
    15781 accctccgcg gaacggtgga atgcgggcgc ccggcgaccg gtttgccaaa accgacctcg
    15841 gtttcgcaga atacatcgat tcctcccgat tcggcacgga ggcgacggac gccggtcccc
    15901 gcggacgggg ccgccggtca taggagtgcg acggatgaac atcggtgtga tcggttccgg
    15961 gtcgatcggt ccggacctgg cgtacggctt cgtctcggcg ctgtcgcgag ccggcggcgg
    16021 cagggtctac ttgcacgaca tccgccgcga ggcgctcgat gccggcctcg agcggatccg
    16081 cgggtacgtc ggcaaggggg tgtcgcgcgg gaagatcagt gccaaggccg cccgcgagat
    16141 cgagcagacg ctgatcccga cgctcgagct ggccgacctc gccggctgcg actacgtgtt
    16201 ggaggcggcg accgaggatc tcgagaccaa gcgcaagatc ctggcccggc tcgagcagac
    16261 cgtgcgggcc gattgtctga tcggcttcgc cacgtcgggc atcccgcgcg cgcgaatcgc
    16321 cgccggcgcc gtgcaccccg aacgctgctt cgtcaaccac ccgttctttc cggcctggcg
    16381 ctcgctgccg gtcgaggtcg tgctctcggg cgatgcgcgc accggcgaac ggatgctcga
    16441 cacgctgacc atgctgggca agatcccggt cgtcaccgcc gacgtaccgt gcttcgccgc
    16501 cgacgacatc ttctgcaatt actgcgccga agcggcgcgc atcgtcgccg agggcgtcgc
    16561 cacgccggcc caggtcgacg cgatcgtcca ccaggcgatc ggcggtggcg gcccgttcaa
    16621 cgtaatggat ctgacccagg gcaacctgct gaacgtgcac tgcctcgagc tgatgcagca
    16681 cgggccggac ggcagcgact ggttcgcgcc gccggcgatc ttcacccggc agggccacaa
    16741 gccgtggatc gaccgcaaga acccgggcga cccgacccac gacgcggcgc tcgccgagcg
    16801 ggtgctcgat cgcatcctcg ccgtgctgct cggacgaacc tactacgtgg tcgaccacga
    16861 gatctgcaac ccgaccgagc tgaactggct gacgcgcacc gcgctcggct tccgcgacgg
    16921 cttgctcgat ctcgccgcat cgctgggcgc cgaccgcgtg cgggagatct gcacgagcta
    16981 cgccgcgagc caccccggct acgtcgtgcc cgacagcatc gccgagcggc gaccgctggc
    17041 gttccacggc aacgtcaggc tcgaccacga tggtgagatc gccgtcgtca ccgtctttcg
    17101 tccggaggtc aagaacgcgc tcgacgagcg gacgatggac gagctggctc gcaccttcgc
    17161 cgcgctcgcc gaggacgacg cgattcgcgg cgtcgtgctg accagccccg acggggcgct
    17221 ggccggtgcc gacatcaccg agctggccgc gctcgccacg ccgcaggaag ccgccgacaa
    17281 gtgccgtcgc ggccaggccg tgctcgaccg gatcgcgacc ttcgccaagc cggtcgtcgc
    17341 cgcggtcgac ggtccggtgc tcggcggggg cgccgagctg tcgatggcct gccacgcgcg
    17401 cgtcgtcggt cgctcgctga tgctgggtca gccggaggtc aacctcggga tcatcccggg
    17461 ctacggcggc acgcagcgcc tgccgcgact cgtcggtgtc gagcgcggac aggaactgtt
    17521 gcgcaccggc cggccggtcg gcccgcgcga ggcgtgcgag tggggatggg cccacggcga
    17581 accggcgggc aagccggtcg ccgtcgccaa ggagctggtc cgccgccacc tcgccgggga
    17641 ggtccagctc gccgcggtct ccgccgcacc gatggacctt ccagagcgac tcgcgccggt
    17701 ggcgctcggc caccggtcgc tcgccgtcga cgcgatcctc gtcgacacgt tgcggcgcgg
    17761 gctgacgcgg ccgcttcccg atggcttggg ggtcgaggcc gagaacttcg cgcgctgcca
    17821 gcagacggtc gactacgaca tcgggatgaa gaacttcatg cagaacggcc cacgtgttcc
    17881 ggccgtgttc atcaacgagt agcggaggcg atgatggcga gcaacgaatc gatcgtgatc
    17941 ctcgacgggg cgcggcgcac accgcgcgcc gacatcctga tcgacaacaa ggagccgggg
    18001 ctgttctcgc ggttcaccgc gacgcagctg ggcggtatgg cgatcgccgc gacgctcgag
    18061 cacacgccga tcgaccccgc cttgatcgga cacgtcgtga tggggatggc gcagcacgcc
    18121 aaccgcgact cgatctacgg tgcccagggc atgcgctggc gcggcggtct gggacaggac
    18181 gtcccggcac tgacggtggc gcggatctgc ggcagcggtg cggaggcggt cgccgtcggt
    18241 tgcgagatga tcctggccgg cgtgcgtcac gaccgcaagc ggccgttcag cgtggtcggt
    18301 ggggcggaga gcatgcagta cccgttctgc ctgttcaact atcgcggccg tcccgtcggc
    18361 gaggcggtgc agaagtacgg cccgatcgac gtcaaggcgc tcccgccggg cacccatctc
    18421 caggacgcgc tgctgatggg gctgtacgac ccgggcgccc ggatggcgat ggccaacacc
    18481 gccgaggagc tcggccggcg ctacgagatc acgcgcgtcg aggccgacga gttcggcttc
    18541 cgttcgcacc ggctggccaa gcaggcgcgt gaggcgggct ggttcgacga ggagatcgaa
    18601 ccggtccggg tcgaacccga cgggtcgagc gagccgatcg aggtgcgata cgacacgcac
    18661 atcctggacg acgtcagtct cgagaagatg gcgcgattgc ggccggcatt cgagcccggc
    18721 gggatcatca ccgcgggcaa cgcgagcgcg gtggtcgacg gcgcggcggc gatggtcatc
    18781 ggcaaacagt cggacgccga ggagcacggc ttgaggccgc tggcccgcgt ccttggcttc
    18841 ggtgtcgcgg cgtgcgatcc ccgcatcatg ggatggggcc cggtccccgc cgcacgacgc
    18901 gcgttcgagt cgagcgggat cgacgccgcg cagatcgacc tggtcgagtt gaacgaggcg
    18961 ttcgcgccgc aggcgctcgc ggtgatccgc gacttcgaga ggatgggaat cgacccgcag
    19021 aaggtcaacc cgatgggcaa cgccatcgcg ctgggccacc cactgggcgc gaccgggtcg
    19081 atcctgaccc tgacctgcgc ctatgccctc aggcgcaccg gccagcgcta cggcatggtg
    19141 acgatgtgca tcggcggcgg gcaggggatc gcgctgattc tcgagtcgat gtaggccacc
    19201 gacgccgatg gggccgatcg acgagcgacg cgcgctggac cacctgatcg agctgctgtc
    19261 ggtcgagggc ccgagcggcg gggagcatca cgtctcccag acgatccgtg ccagggcgcg
    19321 cgcggccggc tgcccggcgg cgtcgatcat cgaggacgac gcgcacctgc gcatcggcga
    19381 cggtttccgc acgggcaacc tgatcatccg catgcccggc acc
//
LOCUS       DASPGN010000253         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1683142, whole genome shotgun sequence.
ACCESSION   DASPGN010000253 DASPGN010000000
VERSION     DASPGN010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1683142"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VD788_07465"
     CDS             complement(<1..455)
                     /locus_tag="VD788_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXV76143.1"
                     /translation="MTADVIDVGVVGVGHLGRHHARIYAELPGTRLVGVVDRDRERAE
                     AIAERHRCRVFDRPADLIGQVRAASVAVPTEQHLAVAEPLLQGGVDLLIEKPLASTLD
                     GVDRLLELAERGSRVLMVGHTERFNPAVIALLRAVDAPRFFEIHRLAGFS"
     gene            complement(452..1222)
                     /gene="lpxA"
                     /locus_tag="VD788_07470"
     CDS             complement(452..1222)
                     /gene="lpxA"
                     /locus_tag="VD788_07470"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR01852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HXV76144.1"
                     /translation="MIDERAVVDPRAQLGRDVVVGPFCAIGPEVTIGDGCILDSHVVV
                     RGPTRIGQGNKISPMASIGGPPQDLKYAGEPTSLVIGDRNRIHEFVTLNRGTAGGGGI
                     TRIGDDNLMMAYTHIGHDCQIGSRVIFANAATLAGHVEVGDDATIGAFSGVHQYCRIA
                     AHAFIGGYSVITQDALPWVTTVGNRAKTYGLNIVGLKRKGYSKETIVALKRCYMILFR
                     SATPFDEALERVEAELGDVAEVRYFVEFIRGSRRGVVR"
     gene            complement(1239..1676)
                     /gene="fabZ"
                     /locus_tag="VD788_07475"
     CDS             complement(1239..1676)
                     /gene="fabZ"
                     /locus_tag="VD788_07475"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HXV76145.1"
                     /translation="MLDIGAILRSLPHRYPFLLVDRVIELRPRQRITAYKNVSINEPF
                     FQGHFPGAPIMPGVLIIEAMAQAGAVLFLHEMKDRDSKTVYFTGIDRARFRRTVVPGD
                     RLRLTVDVIKLRSKTCKMKGRAEVDGELVAEAELLSAIVERGE"
     gene            complement(1680..>2512)
                     /gene="lpxD"
                     /locus_tag="VD788_07480"
     CDS             complement(1680..>2512)
                     /gene="lpxD"
                     /locus_tag="VD788_07480"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXV76146.1"
                     /translation="PLLEVAEPYLALAQVIELFHPAEARPASVSQRAEIAADAVLGTD
                     VSVGPFAVIEAASVIGDRVRIGAGTVVGRGSRVGDDSRLMPRVVLYPGTVVGRRCIIH
                     SGVVLGGDGFGFATSAGRHHKVPHIGHVAVGDDVEIGANTTVDRGALTATEIGSGSKL
                     DNLVMIAHGVVVGDGCLIAAQSGIAGSARVGARVTLAGQVGVVGHVKIADGTVVASKS
                     AVMQDVERGQLIAGIPAVDHREWKRAQALSRKLPELRAEMRELSRRIDQLEHRSPEED
                     "
BASE COUNT          404 a          903 c          805 g          400 t
ORIGIN      
        1 gagaacccgg ccagccgatg gatctcgaag aaccgcgggg cgtcgactgc gcgtaacagc
       61 gcgatcaccg ccgggttgaa gcgctcggtg tggccgacca tcagcacgcg agagccgcgc
      121 tcggcgagtt cgagcagtcg atcgaccccg tccaacgtcg atgcgagcgg cttctcgatc
      181 agcagatcga cgcccccttg cagcagcggc tcggcgacgg ccaggtgctg ctcggtcggc
      241 accgcgacgc tggccgcccg cacctgcccg atcagatcgg ccggtcgatc gaagacgcgg
      301 cagcggtggc gctcggcgat cgcctcggcc cgctcgcgat cgcggtcgac gactccgacc
      361 agtcgcgtgc ccggcagctc ggcgtagatc cgtgcgtggt gccgccccag gtgaccgacg
      421 ccgaccacgc cgacgtcgat cacatcggcc gtcatcggac gaccccacga cggctgccgc
      481 ggatgaactc gacgaaatac cgcacttccg cgacgtcgcc cagctccgcc tcgactcgct
      541 cgagcgcttc gtcaaacggc gtcgcagaac gaaacaagat catatagcag cgcttgagtg
      601 cgacgatcgt ctctttcgag tagcccttgc gcttcagccc gacgatgttc aggccgtagg
      661 ttttcgctcg gttgccgacc gtcgtgaccc acggcagcgc gtcttgggtg atcaccgagt
      721 acccgccgat gaacgcgtgc gctgcgatgc gacagtactg gtgaacgccg ctgaacgcac
      781 cgatcgtcgc gtcgtcgccg acctcgacgt ggccggcgag cgtagccgcg ttggcgaaga
      841 tcacgcggct gccgatctgg cagtcgtggc cgatgtgagt gtaggccatc atcaggttgt
      901 cgtcgccgat acgggtgatc ccgccgcccc ccgccgtgcc gcgattgagc gtcacgaact
      961 cgtggatgcg gttccggtcg ccgatcacga gcgacgtcgg ctcgccggcg tacttcagat
     1021 cctgcggtgg accaccgatc gaggccatcg gcgagatctt gttgccttgg ccgatccgcg
     1081 tcggtccgcg aacgacgacg tgcgagtcga ggatgcagcc gtcgccgatc gtcacctcgg
     1141 gcccgatcgc gcagaacggg ccgacgacga catcccggcc gagctgcgca cgcgggtcga
     1201 ccaccgcgcg ctcgtcgatc atcgccgtcc gcgccacctc attcgccccg ctcgacgatc
     1261 gccgaaagca gctccgcttc cgccaccagt tctccgtcga cctcggcccg gcccttcatc
     1321 ttgcaggtct tcgaccgcag cttgatcacg tcgaccgtca gacgaagtcg gtccccggga
     1381 acgacggtac ggcggaaacg ggcccggtcg atcccggtga agtagaccgt tttcgagtcg
     1441 cgatccttca tctcgtgcag aaacagcacg gcgccggcct gcgccatcgc ctcgatgatc
     1501 aacacgccgg gcatgatcgg cgctcccgga aagtgtccct ggaagaacgg ctcgttgatc
     1561 gagacgtttt tgtaggcggt gatccgctgc cgcggccgca gctcgatcac acggtcgacg
     1621 agcaagaacg gatagcggtg cggcaggctg cggaggatcg cgccgatgtc gagcaacgtt
     1681 cagtcctcct cgggggatcg gtgctcgagc tggtcgattc gccggctcag ctcgcgcatt
     1741 tccgcgcgga gctccggcag cttccggctc agcgcctgcg cacgcttcca ctcgcgatga
     1801 tcgaccgccg gtatcccggc gatcagctgc ccgcgctcga cgtcctgcat cacggcgctc
     1861 ttggaagcga cgacggttcc gtcggcgatc ttcacgtgac cgacgacacc gacttgcccg
     1921 gccagagtga ctcgagcccc gactcgcgcg ctgccggcga tcccgctttg ggcggcgatc
     1981 aggcagccgt cgccgacgac cacgccatgg gcgatcatca ccaggttgtc gagcttgctc
     2041 ccgctgccga tttcggtcgc ggtcagcgcg ccccggtcga ccgtcgtgtt ggctccgatc
     2101 tcgacgtcgt cgccgacggc gacgtggccg atgtgcggca ccttgtgatg gcgcccagcc
     2161 gaagtggcga aaccgaaacc gtcgccgccg agaacgacgc cggagtggat gatgcagcgt
     2221 cgtccgacga ccgttccggg gtaaagcacg acccgcggca tcagccgcga atcgtccccg
     2281 acccgactgc cgcgcccgac gaccgtgccc gcgccgattc ggacccgatc gccgatcacc
     2341 gacgccgcct cgatcacggc gaacggtccg acgctgacat ccgtccccag cacggcgtcg
     2401 gccgcgatct ccgcccgttg cgagaccgac gccggccgcg cttccgccgg gtggaacagc
     2461 tcgatcacct gcgccagcgc caagtacggc tcggcgacct cgagcagcgg cc
//
LOCUS       DASPGN010000254         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_980130, whole genome shotgun sequence.
ACCESSION   DASPGN010000254 DASPGN010000000
VERSION     DASPGN010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980130"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VD788_07485"
     CDS             <1..388
                     /locus_tag="VD788_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76147.1"
                     /translation="GTAVWTAGDADVPGLMARVVLYDMPDAVDADACLAALGARLDLA
                     SDAVGGVRRYRLGARPVIEYEVREHRGVEVNQRNLHGYLYHDRACAEVHVSFIGYQGQ
                     PHMLLERVITSVEIVATDGTDGGDSQ"
     gene            385..1215
                     /gene="ttcA"
                     /locus_tag="VD788_07490"
     CDS             385..1215
                     /gene="ttcA"
                     /locus_tag="VD788_07490"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HXV76148.1"
                     /translation="MSGGDRLLGIEDVDPRALRQRLSRTMLRTVAEHALIEDGDRILV
                     AVSGGKDSYTMLDLLDRARPRAPVGFELIAFHLDQAQPGYDGAALCGWLERSGVTFEI
                     VREDTYSVVIERAASDPGAAYCRVCSRLRRGIVYRAAERLGCNKVALGHHRDDALETL
                     LLNLFYAGRLQAMPAIYTTNDGRFRVIRPLIECAESDIARHAAQENFPILPCNLCGSQ
                     AELKRREVAQILEQLERRIPNLRQVMLAALKNVRPSHLLDADVAEAWSLRAASYAPRR
                     "
     gene            1375..2040
                     /locus_tag="VD788_07495"
     CDS             1375..2040
                     /locus_tag="VD788_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76149.1"
                     /translation="MRSIRWLLILLVLVTVDGASRPARAGVDVDFGARVSLGDRADLY
                     LSISSHYFDRDRQTVDHWHGRFHDPDDLAVALFISHHTGQSLDVLWALYQRRGLSWWE
                     ISLRYGMPVDLWFVEVERAPGPPYGKAYGHWKKHRADDRRVTFSDADLRNLVAVRMAH
                     EYYGVPVELAMEWRAGGKDVRQLMCDEYERRHGARHAGPSVANGPSADKPGRGHGKGG
                     KHK"
     gene            2109..>2475
                     /locus_tag="VD788_07500"
     CDS             2109..>2475
                     /locus_tag="VD788_07500"
                     /inference="COORDINATES: protein motif:HMM:NF025268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXV76150.1"
                     /translation="MTIRPALSLLLLIATGLIGCAGAGGTRTASVPTSLDEAQALAAA
                     SGKPLLVDFYTDWCSACALFAADGEADERLIAALDEVVLVKIDAESEGDGQRLAKEFA
                     VHGYPTFIVADARGETIARW"
BASE COUNT          370 a          824 c          890 g          391 t
ORIGIN      
        1 cgggacggcg gtgtggacgg ccggtgacgc cgacgtgccg gggttgatgg cgcgtgtcgt
       61 gctgtacgac atgccggatg cggtcgacgc cgacgcgtgc cttgccgcgc tcggagcacg
      121 gttggatctc gcgtcggacg cggtcggtgg ggtccggcgc tatcgccttg gcgccaggcc
      181 ggtgatcgag tacgaggtcc gtgagcaccg cggcgtcgaa gtgaaccagc gcaacctgca
      241 cggctacctg taccacgacc gcgcatgcgc cgaggtgcac gtgtccttca tcggttacca
      301 aggccaaccg cacatgctgc tcgaacgggt gatcacgtcg gtcgagatcg tcgcgaccga
      361 cgggacggac ggaggcgatt cgcaatgagc gggggcgacc ggctgctcgg catcgaagac
      421 gtcgacccgc gagcgctgcg ccagcggctg tcgcggacga tgctgcgcac cgtcgccgag
      481 cacgcgctga tcgaggatgg cgaccggatc ctggtcgctg tctccggcgg caaggacagc
      541 tacacgatgc tcgatctgct cgaccgcgcg cgccctcgcg caccggtcgg gttcgagctg
      601 atcgccttcc atctcgacca agcgcagccc ggctacgatg gtgccgcgct ctgcggctgg
      661 ctcgaacggt caggggtgac cttcgagatc gtgcgcgagg atacctactc ggtcgtgatc
      721 gaacgcgccg cgagcgaccc cggcgcggca tactgtcgcg tctgctcgcg cctgcggcgg
      781 ggcatcgtct atcgcgcggc cgagcggctc ggctgcaaca aggtcgctct gggccaccac
      841 cgcgacgatg cgctcgagac gctgctgctc aacttgttct acgcgggccg gctgcaggcg
      901 atgcccgcga tctacaccac caacgacggg cgctttcgcg tgattcgccc gttgatcgag
      961 tgcgccgaga gcgacatcgc gcgccacgcg gcgcaggaga acttcccgat cctgccgtgc
     1021 aacctgtgcg gctcgcaggc agagctcaag cgccgcgagg tggcgcagat cctcgagcag
     1081 ctcgagcgac ggatacccaa tctgcgtcag gtgatgctcg ccgcgctgaa gaacgtgcgg
     1141 ccgtcgcacc tgctcgacgc cgacgtggcc gaggcttggt cgctgcgggc ggcgagctac
     1201 gctccgcggc gttgagccgg ccccggtcgc ggaactcctt gcatccggtc aacgatcgag
     1261 ggttgacggt ttcgtaatcg cgacccgcgc ccgtcgtccg ttgccggggc gccgccccga
     1321 cctagatttg atcgtcgagc gccggcggac gatcggcggt cggaggtggc aacgatgaga
     1381 tcgatacgtt ggctgctgat cttgctcgtc ttggtgaccg tcgacggtgc gtcgcgtccg
     1441 gcgcgagccg gcgtcgacgt ggatttcggc gcgcgggtct cgctcggcga tcgcgccgat
     1501 ctctacctgt cgatctcgtc gcactatttc gaccgggacc gtcagaccgt cgaccactgg
     1561 cacggccggt tccacgatcc ggacgatctg gcggtcgcgc tgttcatctc gcaccacacc
     1621 ggtcagtcgc tcgacgtgct gtgggcgctg taccagcggc gcgggctgag ctggtgggag
     1681 atctccctgc gctacggcat gccggtcgat ctgtggttcg tcgaggtcga gcgcgcgccg
     1741 ggtccgccgt acggcaaggc ctacggccac tggaagaagc accgcgccga cgaccggcgg
     1801 gtcacgttca gcgacgccga cctgcgcaac ttggtcgcgg tccgcatggc ccacgagtac
     1861 tacggggtgc cggtcgagct ggcgatggaa tggcgcgccg gcggcaagga cgtccggcag
     1921 ttgatgtgcg acgagtacga gcggcgacac ggcgcgcgac acgcggggcc gtccgtcgcg
     1981 aacggcccct ccgcggacaa gccgggccgc ggccacggca agggcggcaa gcacaagtag
     2041 cccggccgcg tacgcggcac gatggtagac tgcgtccgcc tgcgggcaat cgacgccagg
     2101 aggacgcggt gaccattcga ccggcgctga gcctgctgct cctgatcgcg accgggctga
     2161 tcgggtgcgc cggcgcaggc ggtacgcgca cggccagcgt cccgacctcg ctcgacgagg
     2221 cgcaggcgct cgccgccgcc agcggcaagc cgttgttggt cgatttctac acggattggt
     2281 gcagcgcatg cgcgctgttc gccgcggacg gcgaggccga cgaacgattg atcgccgcgc
     2341 tcgacgaggt cgtgctggtc aagatcgacg ccgagagcga aggcgatggc cagcggttgg
     2401 ccaaggaatt cgcggtgcac ggctacccga ccttcatcgt cgccgacgct cggggcgaga
     2461 cgatcgcgcg atggt
//
LOCUS       DASPGN010000255        11579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_161002, whole genome shotgun sequence.
ACCESSION   DASPGN010000255 DASPGN010000000
VERSION     DASPGN010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11579
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161002"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /gene="tuf"
                     /locus_tag="VD788_07505"
     CDS             <1..141
                     /gene="tuf"
                     /locus_tag="VD788_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HXV76151.1"
                     /translation="EMVMPGDNVTIEVNLITPIAMDKGLRFAIREGGRTVGAGTVTEI
                     VE"
     gene            187..336
                     /gene="rpmG"
                     /locus_tag="VD788_07510"
     CDS             187..336
                     /gene="rpmG"
                     /locus_tag="VD788_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXV76152.1"
                     /translation="MRDNVTLQCSSCKRRNYVTTKNKKTTTGKLEFSKYCRWCRKHTP
                     HKETR"
     gene            356..559
                     /gene="secE"
                     /locus_tag="VD788_07515"
     CDS             356..559
                     /gene="secE"
                     /locus_tag="VD788_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXV76153.1"
                     /translation="MMERFRRMKQFLVEVVSELKKTTWPGRREVYGTTVVVIVAVVIC
                     AVYLWVVDMVLGRAMNYVFQVFG"
     gene            559..1104
                     /gene="nusG"
                     /locus_tag="VD788_07520"
     CDS             559..1104
                     /gene="nusG"
                     /locus_tag="VD788_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HXV76154.1"
                     /translation="MTQAAVDKRWYIVHTYSGFEAKVKESLTQRAEAMGMADVIEEIL
                     IPTEEVVEVREGKKTRSTRKFFPGYVLVKMVMSDDAWHVVRNTPKVTGFVGTGNKPVP
                     LSDAEVERIVNQMSVAAEKPKPKFEFRIGEQVRIVDGPFSNFTGQVEEINEDRSTLKV
                     MVTIFGRATPVELEFLQVERP"
     gene            1129..1548
                     /gene="rplK"
                     /locus_tag="VD788_07525"
     CDS             1129..1548
                     /gene="rplK"
                     /locus_tag="VD788_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HXV76155.1"
                     /translation="MKKVVGNIKLHIPAGKATPAPPVGPALGQHGVNIMDFCKAFNAR
                     TAKDGGILTPVVITVYQDHSYSFVTKTPPASVLLAKAAGVPKGSGTPNRDKVGKVTRA
                     QVEEIARAKMQDLNAESIEAAMRIIEGSARSMGIEVG"
     gene            1581..2276
                     /gene="rplA"
                     /locus_tag="VD788_07530"
     CDS             1581..2276
                     /gene="rplA"
                     /locus_tag="VD788_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HXV76156.1"
                     /translation="MARHGKNYVAARKAVERRMHGLEEGVNAVRRAAFAKFDETLELN
                     LRLGVDPRHADQMVRGTVVLPHGTGKSKRVLVIAGGDKAKEAEEAGADHVGGAEMAKK
                     IQDGWLDFDAVIATPDMMREVGKLGKVLGPRGLMPNPKTGTVTFDVKQAVAEIKAGKV
                     EFRVDKTAIIHVAVGKLSFSDEQLRENAQTLIAAVVKAKPASAKGKYVRSIYLASTMG
                     PGVEIDPAAVDAA"
     gene            2290..2823
                     /gene="rplJ"
                     /locus_tag="VD788_07535"
     CDS             2290..2823
                     /gene="rplJ"
                     /locus_tag="VD788_07535"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HXV76157.1"
                     /translation="MNRADKAALIETLNGEFSAAPHVIVASFRGLTVNQANELRRRVS
                     RAGGRYRVIKNRLARRAASGTPVEKLSRTFVGPCAVATHDSDPVALAKTLTEFVKDNP
                     QVELVSGLVDAKETIDASGVKQLATLPGLSELRAQLLALVQTPATTLVRLLNTPGAQI
                     ARVVDARREGRTGGGTE"
     gene            2887..3264
                     /gene="rplL"
                     /locus_tag="VD788_07540"
     CDS             2887..3264
                     /gene="rplL"
                     /locus_tag="VD788_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXV76158.1"
                     /translation="MADLEAIAENLSSLTVIEAANLVKMLEEKWGVSAAAPVAIAAAG
                     AGPAVAEAAEEKTEFEVILAEVGAKKINVIKAVREVTNLGLKEAKDLVDGAPKPIKDG
                     VTKQEAEEIKKKFEEQGAKVEIK"
     gene            3424..7881
                     /gene="rpoB"
                     /locus_tag="VD788_07545"
                     /pseudo
     CDS             3424..7881
                     /gene="rpoB"
                     /locus_tag="VD788_07545"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
     gene            7934..>11579
                     /gene="rpoC"
                     /locus_tag="VD788_07550"
     CDS             7934..>11579
                     /gene="rpoC"
                     /locus_tag="VD788_07550"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXV76159.1"
                     /translation="MSRPAFLFDKAKTINDFNAIRISLASPEKIRSWSYGEVTKPETI
                     NYRTFKPERDGLFCAKIFGPVTDWECLCGKFKRMKHRGVVCDKCGVEVTQSKVRRERM
                     GHIELASPVSHVWFFKGLPSRIGHLLDMSLRELERILYFEAYVVVDPGDCPEIHEKGI
                     LTEEQYRELQDKYPDGFQAGMGAEAIKELLARVEVDGLATELRERMRNETSQQKRLKF
                     AKRLKVVDAFRKSGNRPEWMILDVIPVIPPELRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKKLLDLKAPDVIVRNEKRMLQEAVDALFDNGRRGRVLRGSNNRPLKSLSDTIKG
                     KQGRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKKMALELFKPFIYNKLEQEGLV
                     STIKAAKEMVELERPEVWDYLEEVIKEHPVLLNRAPTLHRLGIQAFEPTLVEGKAIKI
                     HPLVCTAFNADFDGDQMAVHIPLSAKAQIEAMVLMLSTNNVLSPSNGLPIVNPSQDIV
                     LGCYYLTQENLNAAGSGRSFGSVAEVISAYQAGEVETLTPISLRLSGRLIDLSTLPVK
                     ERDSVVRAPEQAVSNYRLRTTVGRVLFNDALPEGLPFINGLMKKKTLQSLVHYVYLRH
                     GAHLTVALVDQLKDVGFLFATRAGVSIGIDDMVVPGEKARVVDKAKQDQIEVEQQFLD
                     GLITNRERYNKVIAIWSEATEKVSEAMFREMTRVERESKELNPIMMMADSGARGSKQQ
                     MRQLAGMRGLMAKPSGEIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSG
                     YLTRRLVDVSQDVIITSDDCQTTEGIEVSRIEEGGEIIEELRDRIVGRVALEDILDPL
                     NAEVIVRKNEMIDEDLAQTVQETGIESVKIRSVLTCECERGVCIRCYGRNLATSRPVE
                     LGEAVGVIAAQSIGEPGTQLTMRTFHIGGTARIEEQSNIEAANAGTLRYQKLKTVDDR
                     DGRPVAINRNGAIVIVDREGRERERYNVVYGAILDVRDGDVVERGQRLAEWDPYNFSI
                     LTDVPGKIRFHDIEAGVTMKEEVEEVSGLVRQVIIQSQDEKKHPQLLVLDEKGKAQRK
                     ILLPVHATIMSQDGAEVRQGDVLAKIPRATTKTKDITGGLPRVVDLFEARHPKDPAVM
                     AEVDGTVHYGEIVKGSRKVIVRTDDGEEREYAIPKGMHVTVQEGEAVRAGDAFTDGPK
                     DPHKILEILGE"
BASE COUNT         2287 a         3549 c         3988 g         1755 t
ORIGIN      
        1 gagatggtga tgcccgggga caacgtgacg atcgaggtga acctgatcac gccgatcgcg
       61 atggacaagg gcctgcggtt cgcgatccgc gagggcgggc ggacggtcgg cgccgggacc
      121 gtcaccgaga tcgtcgagta gcaggcggat ccgttcggcg acggaacagc gaaggcggtc
      181 aacacgatgc gagacaacgt gaccctgcaa tgcagctcat gcaagcgacg caactacgtc
      241 acgaccaaaa acaagaagac gacgacgggc aagctggagt tcagcaagta ctgccgctgg
      301 tgtcgtaagc acacgccgca caaggaaacc cgctgaccgc gggcggagaa cgacgatgat
      361 ggaacgcttc agacggatga agcagtttct cgtcgaggtg gtctcggagc tgaagaagac
      421 cacgtggccg ggcaggcgcg aagtctacgg cacgaccgtc gtggtgatcg tcgccgtggt
      481 gatctgcgcc gtttatctgt gggtggtcga catggtactg ggccgagcga tgaactacgt
      541 gttccaggtg ttcggatgat gacgcaggcc gcggtcgaca agcgctggta catcgtccac
      601 acctattccg gcttcgaggc caaagtcaag gagagcctta cccaacgcgc cgaggcgatg
      661 ggcatggcgg acgtcatcga ggaaatcctg atcccgaccg aggaggtcgt cgaggttcgc
      721 gagggaaaga agacgcggtc gacgcgcaag ttcttcccgg gctacgtgct ggtcaagatg
      781 gtgatgtcgg acgacgcctg gcacgtcgtg cgcaacacgc ccaaggttac cggcttcgtc
      841 ggcaccggga acaagccggt cccgttgagc gacgccgagg tcgagcggat cgtcaaccag
      901 atgagcgtgg ccgcggagaa gccgaagccg aagttcgagt tccgcatcgg cgagcaggtg
      961 cggatcgtcg acgggccgtt cagcaacttc accggccagg tcgaggagat caacgaagac
     1021 cggagcacgc tcaaggtgat ggtcacgatt ttcggccggg cgacgccggt cgagctggag
     1081 ttcttgcagg tcgagcgccc gtagccgcgg gtcgcgccgg ggtatccgat gaagaaggtg
     1141 gtcggtaaca tcaagctgca cattccggcg ggcaaagcga cgccggcgcc gccggtcggt
     1201 ccggctctcg gccagcacgg cgtcaacatc atggatttct gcaaggcgtt caacgcccgg
     1261 acggcgaagg acggaggcat cctcacaccg gtcgtgatca cggtctacca ggaccattcc
     1321 tactcgttcg tcaccaagac gcctccggct tcggtgctgc tggccaaggc ggccggcgtg
     1381 ccgaagggtt cgggcacgcc gaaccgggac aaggtgggca aggtgactcg cgcgcaagtc
     1441 gaggagatcg ccagggcgaa gatgcaggac ctcaacgccg agtcgatcga agcggcgatg
     1501 cgcatcatcg aaggaagcgc acgttcgatg gggatcgaag tcggctgacg tcgcccgtcg
     1561 cgaccaagga gcaagtggca atggccaggc acggaaagaa ctacgttgcg gcgcggaagg
     1621 ccgtcgagcg acgcatgcac gggctggagg agggcgtgaa cgcggtgcgc cgagcggcat
     1681 tcgccaagtt cgacgagacg ctcgaactca acctgcggct gggtgtcgac ccccgccacg
     1741 cggaccagat ggtgcgcggc acggtcgtcc tgccccacgg cacgggcaag tccaagcgcg
     1801 tgctggtgat cgccggcggc gacaaggcga aggaggccga ggaagccggc gccgaccacg
     1861 tcggcggcgc cgagatggcg aagaagatcc aagacggctg gctcgatttc gacgcggtga
     1921 tcgccacgcc cgacatgatg cgcgaggtcg gcaagctggg gaaggtgctc ggcccccgcg
     1981 gcctgatgcc gaaccccaaa accggaaccg tgacgttcga cgtcaagcag gcggtggcgg
     2041 agatcaaggc cggcaaggtc gagtttcggg tcgacaagac ggcaatcatc cacgtggcgg
     2101 tcggcaagct ttcgttcagc gacgagcagc tccgggagaa cgcccagacg ctgatcgcgg
     2161 cggtcgtcaa ggccaaaccg gcgtcggcca aaggcaaata cgttcgatcg atctacctgg
     2221 cctcgacgat ggggcccggt gtcgagatcg atccggcggc ggtcgacgcc gcctgagtcg
     2281 aggtgtaagg tgaaccgcgc ggacaaggca gcattgatcg agactttgaa cggagaattc
     2341 tccgcagcgc cgcacgtgat cgtggctagc tttcgcggcc tgacggtcaa ccaggccaac
     2401 gagctacgcc gtcgcgtgag ccgcgccggt gggcgttacc gggtgatcaa gaaccgactc
     2461 gcccggcgcg cggcgagcgg aacgcctgtc gagaagttga gtcggacgtt cgtcggaccg
     2521 tgcgcggtcg cgacccacga cagcgacccg gtggcgctgg cgaagacgct caccgagttc
     2581 gtcaaggaca acccgcaggt cgagttggtc tccgggttgg tcgacgccaa ggagacgatc
     2641 gacgcgtctg gcgtcaagca gctggcgacg ctgcccggtc tgagcgagct gcgcgcgcag
     2701 ttgctggcgc tggtacagac gccggccacc acgctggtgc gattgctcaa cacgccgggc
     2761 gcgcagatcg cgcgggtcgt cgatgcgcgg cgagaggggc gaaccggcgg gggtacggaa
     2821 taggcccgcg agtcttgggc aggtcaggag attcattcac cgagtccgaa cggaggagtt
     2881 agcgctatgg ccgatctgga ggcaattgcc gagaacctga gttcgctgac cgtcatcgag
     2941 gcggcgaacc tggtcaagat gctcgaggag aaatggggcg tctccgccgc cgcgccggtc
     3001 gcgatcgccg cggcgggagc cggtccggcg gtcgccgagg cggcggagga gaagaccgag
     3061 ttcgaggtca tcctggccga ggttggcgcc aagaagatca acgtgatcaa ggccgtccgc
     3121 gaggtcacga acctcgggct caaggaggcc aaggacttgg tcgacggtgc gcccaagccg
     3181 atcaaggacg gcgtgaccaa gcaagaggcc gaggagatca agaagaagtt cgaggagcag
     3241 ggagcgaagg tcgagatcaa gtaggtttgg agactgtcgc tcgatcccaa ttcggtttcg
     3301 tcgcagaccg tgacctcggc ggtctcgggg gggtgggtct gttcgcccgt cctcttcgtc
     3361 cattctgatc cggagcggag cgcagccgcg gcgccccggc ttccttaagg agtgtcaacc
     3421 ccgatgtccg agacgctcac cggttcccgc ctgcgggcgg acttctcgaa gatccgcacg
     3481 cagatcccga tcccgaattt gatcgaggtc cagaagcgct cctacgaacg ttttctgcag
     3541 atgcacgccg cgcccgccga ccgcgacccg caggtcggcc ttcagggcgt tttcaagtcg
     3601 atcttcccga tcaaggactt ccgcgagacc tgctcgctcg agttcgtcga gtacacgatc
     3661 ggcaactggg agtgcaagtg cggtgagctg caggggatcg agaacctgcg cgccgaatgc
     3721 aaattctgtg gacaccggtt ggtggctccc gacgcgcgcg gcggggtcat cacctgccgg
     3781 gcgtgcggca acctgacgcc cgtctcggtc cgcgagtgcg acaactgcgg cgaagcggtc
     3841 cggctcaagt tcaagtacga cgtcgaggag tgccaagagc gcggtcagac ctacaccgtt
     3901 ccgctcaagg tcacgatcca gctcgtggtc tacgacaagg atcccgaaac cgagaccaag
     3961 agcattcgcg acatcaagga gcaggaggtc tacttcggcg agatcccgat gttgaccgac
     4021 aacggcacct tcatcgtcaa cgggacggaa cgggtgatcg tcagccagct ccaccgcagc
     4081 ccgggagtgt tcttccaggc cgacgcgagc cggacctcgt acatggcgaa ggtcatcccg
     4141 taccgcggtt cgtgggtcga gttcgagacc gaccacaaga acatcctcta cgtccgcatc
     4201 gaccgcaagc gcaagttccc cgccaccgtc ttcctccgcg ccctcggcat cgaggacgac
     4261 cgttcgatcc tcaacacgtt ctacacgccg gcgatcgtgc gtctggccga cggcaagtgg
     4321 gagttcaagg tcggcccggg tctcgtcggt cgcaagactc gcgtcgcggt caccgacaag
     4381 aagggcacgg agctgatcaa gaagggccgc cgcgtcaagg aagaccagct cgcggagctg
     4441 cgcgacgcca agatcgaatg ggtgccggtc gccggtgcgg aagcggaggg cgcctacacc
     4501 gccagtcatc tcgtcgacca ggagaccggc gaggtgatgt tcgaggcggc ccacccgctc
     4561 accgaggaga cgctgaccaa cctgcaggcc cgcggcttca acgagttcga ggtggttttc
     4621 cccgaccggg aggaggtcgg tgactcgctg ctcgcgacga tggccaagga tccggtgaag
     4681 gaccagcacg aggcgttgat cgagatctac cggcgactgc gaccgggcga cccgccgacc
     4741 gtcgagtcgt cgcgcacgct gttcaacaac atgttcttcg atccggcgcg ttacgatttc
     4801 tcgcgcgtcg gtcggttgaa gttcaacacc aagctgtacg ggtcgacccc cgggcacaat
     4861 ctcgacgacg gaccggaccc gatgtggccg gacgatatca agcagctgtg ggagcgcggg
     4921 gaggaccgca caccgtcccg ccaggatttc gtcgaggtgc tgcgctacat gttgaagcta
     4981 cgtcgcggca tcgggcatct cgacgatatc gatcacctgg gcaatcgccg cgtacgcagc
     5041 gtcggcgagc tgctggagaa ccagttccgc atcgggctcg tacgaatgga acgggcgatc
     5101 aaagagaaga tgtcggtcta tcaagagatg acgacggcga tgccgcacga cctgatcaac
     5161 gccaagcccg tgatggcggc gatccaggag ttcttcggca gcagccagct gtcgcagttc
     5221 atggaccaga ccaacccgct gtcggaggtg acgcacaagc ggcggctgtc ggcgctgggg
     5281 cccggcggcc tgtcgcgcga gcgggcgggg ttcgaggtgc gtgacgtcca tccgacgcac
     5341 tacggccgga tttgcccgat cgagacaccc gagggtccga acatcggcct gatctcgtcg
     5401 ctgtcgtgct atgcacgcat caacgacttc ggcttcatcg agtcgcccta ccgcaaggtg
     5461 gagagcggca aggtcgccga cttcgtccgc gtgacgcgca ccgggcagag caactaccgt
     5521 ctcgacgagg tggtgcccgc cgccaagttc gcaccggagc gcgagcgggt tcgggccgcg
     5581 agtaagttgc ccccggaggc cgtgccgcac tcgttctacc tgtcggccta tcaggaagac
     5641 cgcgagacga tcgcgcaggc caacgccagc ctgaccgatg acggccactt cgtcaacgag
     5701 cgggtcaacg cgagaaagca gggtgagttc aagctggtac cccgggagga ggtcagctat
     5761 atcgacgtct cgcccaagca gctcgtgtcg gtcgcggcat cgttgatccc gttcctcgag
     5821 aacgacgacg ccaaccgcgc cctgatgggc tcgaacatgc agcgccaggc cgtgccgctg
     5881 ctccggccgc aggccccgtt cgtcggtacg ggcatggagg gcatcaccgc ccgggactcg
     5941 gggtcggtcg tcacctgcaa gcgggcgggc gtggtcgacc tggtcgactc ccagcggatc
     6001 atcgtccgcg tcggtgcgca cggccaggag gatttcggcg ccgacatcta ctcgttgatc
     6061 aagttcaaac gatcgaacca gaacacctgc atgaatcagc gtccgctcgt ccgcgaagga
     6121 cagcgggtcg acgagggaga ggtgctggcc gacggcccgt gcaccgaaaa cggcgagctg
     6181 gccctcggtc gcaacatcct cgtcgcgttc atgccgtggc gcggttacaa cttcgaggac
     6241 gcgatcctcg tgtcggaacg gctcgtgcgc gaggattcct acacctcgat ccacatcgag
     6301 gagttcgaca tcgaggcgcg cgacaccaag ctcggacccg aggagatcac gcgcgacatc
     6361 cccaacgtct cggaagagtt cctcaaggac ctcgacgagg cgggggtgat ccgcatcggc
     6421 gctcgggtca aaccgggcga catcctcgtc ggcaaggtga cgcccaaggg tgagacccaa
     6481 ctgacaccgg aagagaagct gctgcgggcg atcttcggtg agaaatcggg tgacgtccgc
     6541 gacgcgtcgc tgaccacccc gcccggcatc gagggcatcg tcgtcgacgt caaggtcttc
     6601 agccgcaagg gcgtggagaa ggaccagcgc gcgctgcaga tccaggccga ggacgagcaa
     6661 cgcatgcgca aggacctcgc cgaccaggtg cggatcatcc gcgaggaaga ccgcaagctg
     6721 ctgatggagc tgctggaggg cgagacgctg gtcgcgccgc tcgtcagccg ccggcgcggg
     6781 gagacgctgc tcgaggcggg caccgagctg actcgcgagc ggatgtcggc gatcgagacc
     6841 gaggatctga ggacctgcga gttcaagccg tccaaggcgg aaaagcagcg ggaggtgcac
     6901 cgcatcgagg agcgcacgcg caagcggatc gcgattctcg agaagctggt caaggagaag
     6961 atcgagctga tggagaaggg cgacgagctg gctcccggcg tgatcaagat ggtcaaggtc
     7021 ttcgtcgcga tgaagcgcaa gctggccgtc ggcgacaaga tggccggtcg ccacggtaac
     7081 aagggtgtga tcgcgcgaat cctgccttcc gaggacatgc cctacacccc cgacggcacc
     7141 ccggtcgacg tcgtgctgaa cccactcggc gtcccgtcac gaatgaacgt cgggcagatt
     7201 ctggagacgc acctcggctg ggcggcctcg cagcacggcg aatggttcgg ccaacgcctc
     7261 gaccgggaac gctcggtggc agccgccgag atccgcgcgc acttggcggc gttgatcccg
     7321 tcggcgatgc tgcgcgattg gatcggcggg atggacgacg atcgcctgct cgacgtggcc
     7381 cgatcgctgt cgcacggcgt gcggttcgct tcgcccgttt tcgacggcgc cagcgaggac
     7441 gagatcaagc accaactcaa gctcgccgac ctgccgacgt cgggcaagat ccagctgcgc
     7501 gacggacgga ccggcgagcc gttcgagcaa ccggtgaccg tcggcttcat ttacctgatg
     7561 aaactgtcgc atctggtcga cgacaagatc cacgcacggt cgatcggccc gtactcgttg
     7621 atcacacagc agccgctggg cggcaaggcc cagttcggcg gccagcgctt cggcgagatg
     7681 gaggtgtggg cgctcgaggc ctacggcgca gcgcacattc tccaggagct actcaccgtc
     7741 aagtccgacg atgtctatgg caggaccaag atttacgagg cactggtgaa aggcgaaacg
     7801 gcggtcgagc cgggattgcc cgagtcgttc aacgtcctgg tgcgcgagct gcagagcctg
     7861 tgcctggacg tcgagctgct cagcgatcgc gccgatgcca ccagcgcctc gacgatttga
     7921 gaggggaggc ttcgtgagca ggccagcgtt cctcttcgac aaagcgaaga cgatcaacga
     7981 cttcaacgcg atccggatca gcctcgcgag tccggagaag atccgctcgt ggtcctacgg
     8041 cgaggtgacc aagccggaga cgatcaacta ccgcacgttc aagcccgaac gcgacggctt
     8101 gttctgcgcg aaaatcttcg gtccagtgac cgactgggag tgtctctgcg gcaagttcaa
     8161 gcggatgaag caccgcggcg tcgtttgcga caagtgcggc gtcgaagtga cgcagagcaa
     8221 ggtgcggcgc gagcggatgg ggcacatcga gctggccagc ccggtcagcc acgtctggtt
     8281 cttcaagggc ctgcccagcc ggatcggtca cctgctcgac atgtcgctgc gcgagctcga
     8341 gcggattctc tacttcgagg cctacgtcgt cgtcgacccg ggcgattgtc cggagatcca
     8401 cgagaagggg atcctgaccg aggagcagta ccgcgagctg caggacaagt acccggacgg
     8461 cttccaggcc gggatgggtg ccgaggcgat caaggagctg ctggcccggg tcgaggtcga
     8521 cgggctcgcc accgagttgc gcgagcggat gcgcaacgag acttcgcagc aaaagcggtt
     8581 gaagttcgcc aagcggctga aggtcgtcga cgccttccgc aagtcgggta accgcccgga
     8641 atggatgatc ctcgacgtga tcccggtgat cccgccggag ctgcgcccgc tggtgccgct
     8701 cgacggggga cgcttcgcga cgtccgatct caacgacctc taccgccggg tgatcaaccg
     8761 caacaaccgg ctgaagaagc tgctcgacct caaggcgccc gacgtgatcg tccgcaacga
     8821 aaagcgcatg ctgcaggaag cggtcgacgc gctgttcgac aacggccgtc gcgggcgggt
     8881 gcttcgcggc agcaacaacc ggccgctcaa gtcgctgtcc gacacgatca agggcaagca
     8941 gggtcgcttc cgccagaacc tgctcggcaa gcgcgtggac tactccggcc gctcggtgat
     9001 cgtcgtcgga cccgagctga agctgcatca gtgcggcttg ccgaagaaga tggccctcga
     9061 gctgttcaag ccgttcatct acaacaagct cgagcaggaa gggctggtct cgacgatcaa
     9121 agcggccaag gagatggtcg agctcgagcg acccgaggtc tgggattacc tcgaagaggt
     9181 gatcaaggag cacccggtgc tgttgaaccg ggcgccgacg ctccaccggc tggggatcca
     9241 ggcgttcgag ccgacgctgg tcgagggcaa ggcgatcaag atccacccac tggtctgcac
     9301 ggcgttcaac gccgacttcg acggtgacca gatggcggtg cacatcccgc tgtcggccaa
     9361 ggcgcagatc gaggcgatgg tgctgatgct gtcgacgaac aacgtgctga gcccgtcgaa
     9421 cggcttgccg atcgtcaatc cgagccagga catcgtcctc ggctgctact acctgacgca
     9481 ggagaacctc aacgccgccg gcagcggtcg ctcgttcggc tcggtggcag aggtcatctc
     9541 ggcctaccag gcgggcgagg tcgagacgct gacgccgatc tcgctgcggc tcagcgggcg
     9601 gctgatcgat ctgtcgacgc tgccggtcaa agaacgcgac agcgtcgttc gcgcgccgga
     9661 gcaggcggtg agcaactacc ggctgcggac gaccgtcggc cgggtgttgt tcaacgacgc
     9721 gctgccggag ggtctgccgt tcatcaacgg cctgatgaag aagaagaccc tgcagtcgct
     9781 cgtgcactac gtttacctgc gccacggcgc tcacctgacg gtggcgttgg tcgaccagct
     9841 gaaggacgtc ggctttctgt tcgccacccg agccggggtc tcgatcggca tcgacgacat
     9901 ggtcgtcccg ggcgagaagg cgcgtgtggt ggacaaggcc aagcaggacc agatcgaggt
     9961 cgagcagcag ttcctcgacg gcctgatcac caatcgcgag cgctacaaca aggtcatcgc
    10021 gatctggtcg gaggcgacgg agaaggtctc cgaggcgatg ttccgcgaga tgacccgcgt
    10081 cgagcgcgag agcaaggagc tcaatccgat catgatgatg gccgactccg gtgcgcgggg
    10141 cagcaagcag cagatgcggc agctggccgg gatgcgcggg ttgatggcca agccgtccgg
    10201 cgagatcatc gagacgccga tcacggcgaa cttccgcgaa ggcctcaacg tcctgcagta
    10261 cttcatctcg acccacgggg cgcgcaaggg tctggccgat accgcgctga agacggccaa
    10321 ctcgggctac ctcacccgac ggctggtcga cgtgtcgcag gacgtgatca tcaccagcga
    10381 cgattgccag acgaccgagg gcatcgaggt cagccggatc gaggagggcg gcgagatcat
    10441 cgaggagctg cgcgaccgca tcgtcggccg cgtcgcgctc gaggacattc tcgacccgct
    10501 gaacgccgag gtgatcgtcc gcaagaacga gatgatcgac gaggacctgg cgcagacggt
    10561 gcaggagacc ggtatcgagt cggtcaagat ccgttcggtg ctgacctgcg agtgcgagcg
    10621 cggggtttgc atccgctgct acggccgcaa cctcgcgacc agccggccgg tcgagttggg
    10681 cgaggcggtc ggcgtgatcg cggcccagtc gatcggcgag ccgggcacgc agttgacgat
    10741 gcggaccttc cacatcggcg gcacggcccg catcgaggaa cagagcaaca tcgaggcggc
    10801 caacgccggc acgttgcgct accagaagct gaagacggtc gacgatcgcg acggacggcc
    10861 ggtcgcgatc aaccgcaacg gcgcgatcgt catcgtcgat cgcgagggtc gagagcgcga
    10921 gcggtacaac gtcgtctacg gcgcgatcct cgacgtgcgg gacggcgacg tggtcgagcg
    10981 cggccagcga ctggccgagt gggaccctta caacttttcg atcctgaccg acgttcccgg
    11041 caagatccgc ttccacgaca tcgaggccgg ggtcacgatg aaggaagagg tggaagaggt
    11101 cagcggtctg gtgcggcagg tgatcatcca gtcgcaggac gagaagaagc acccgcagtt
    11161 gctcgtgctc gacgagaagg gcaaggcgca gcggaagatc ctgctgccgg tgcacgcgac
    11221 gatcatgagt caggacggcg cggaagtacg ccagggcgac gtgctggcga agatcccgcg
    11281 agcgaccacc aagaccaagg acattaccgg cggcctgccg cgcgtcgtcg acctgttcga
    11341 ggcgcgccac ccgaaagacc cggcggtgat ggccgaggtc gacgggacgg tacactacgg
    11401 ggagatcgtc aagggctcgc gcaaggtgat cgttcgcacc gacgacggcg aggagcgcga
    11461 gtacgcgatt ccgaagggca tgcacgtgac ggtgcaggag ggagaggcgg ttcgtgccgg
    11521 cgacgcgttt accgacggcc cgaaggaccc gcacaagatc ctcgagatcc tcggcgagc
//
LOCUS       DASPGN010000256         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_44300, whole genome shotgun sequence.
ACCESSION   DASPGN010000256 DASPGN010000000
VERSION     DASPGN010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44300"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1449)
                     /locus_tag="VD788_07555"
     CDS             complement(<1..1449)
                     /locus_tag="VD788_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV76160.1"
                     /translation="MARSSLDRYERIRPLGEGGAGRVWLVRDRLLGGRLVALKALGGG
                     DTARSRSLRDEFFALATLRHPNLAEVYEFHDGESPCFTLEYVDGTDLAAFGRSAPAPV
                     LLELIAEALRALAFLHDFTVFHRDVKPANLLVRREPRLGSRLVLLDFGLALIDGTREA
                     GPAGTLPYLAPELFQGTPAGRRSDLYGLAAVIHEVVLGEPPVSLEDDRAIGDFVTRVT
                     SGERRPRVWPDRVPDGLRLWLEMLLATEPGARPASAAEALARLNSLVGTGFPAETHLT
                     RTARLESGAPEGRDETLCSLTDRIGDARGPDVILLAGEAGSGKTRLLAHLAARGIRDG
                     LAVRWHLGPGNEPATGSDRATLVCLDDPERLGSETVDGLCRLARGESRADGRVIATVR
                     AREVGEPTLRRLLDDRELLANLAVVELPPLDRRGIAALAGRLFPGETADSARVAWLEQ
                     HSEGNAAALVALLVDEAWENRRTLAAPVAVRQS"
     gene            complement(1519..1866)
                     /locus_tag="VD788_07560"
     CDS             complement(1519..1866)
                     /locus_tag="VD788_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76161.1"
                     /translation="MRRTHRRQIVFALALLLCTSVGGLNAAASRDPGPATSFAFTGKT
                     KTQAPNPFDFRFSTFAQFFRLAFASADRLHSLGSLPYLGGWWPDRTSLAAHALGKRPE
                     EPEREITDDHVPI"
     gene            2099..2782
                     /locus_tag="VD788_07565"
     CDS             2099..2782
                     /locus_tag="VD788_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV76162.1"
                     /translation="MKILVVEDEPSLREGLRDLLDGAGHQTLTADDGDAGLAAAVDPS
                     IELVVLDLMLPRVDGIEVCRRLRRARPRLPILVLTARGSEDQKVSGLRAGADDYVTKP
                     FGTRELLARIEALARRAIEPRDGPTQIATDGCRIDLGRCLATRDGSAAVQLTARETEI
                     LRRLYVHRTRAVTRAELLETVWGARGDLQTRTVDMTIANLRQKIERDPASPRIVVTVK
                     GVGYAWGES"
     gene            2779..>3357
                     /locus_tag="VD788_07570"
     CDS             2779..>3357
                     /locus_tag="VD788_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76163.1"
                     /translation="MIRPLAGRTAVAAVLTAAGVIVPCVAWYVVGSRAAIERAQRLAE
                     EPNVAARREAARLAQQLALRLESMRVSESRRPFQDYLTADRLRELQRDCSYEITVPSP
                     LAEGPVDPLIWAHFQLDAVGVLTLPTLAEDGPRGGVDDASEIQRAILGELECAGADHL
                     SAFHRSASPSEPRQLPSPLGLITVGPFAWHTAT"
BASE COUNT          529 a         1142 c         1196 g          490 t
ORIGIN      
        1 cgactgccgc accgcgacgg gggccgccag cgtccgccgg ttctcccacg cctcgtcgac
       61 gagcagcgcg acgagcgccg ccgcgtttcc ctccgagtgt tgctcgagcc aggcgactct
      121 cgcactgtcg gccgtctcgc cggggaacag gcgaccggcc agcgcggcga tgccgcggcg
      181 gtcgagcgga ggaagctcga cgacggccag gttcgcgagg agctcccggt catcgagcaa
      241 acgtcgcagg gtcggctcgc ccacctctcg cgcacggacg gtggcgatca cccgcccgtc
      301 ggcacgcgac tccccgcgcg ccagccggca aagcccgtcg accgtttcgg agccgagcct
      361 ctccggatcg tcgagacaga ccagcgtggc ccggtcgctc ccggtcgccg gctcgttgcc
      421 cgggcccagg tgccagcgga ccgcgagtcc gtcgcggatg ccgcgggccg cgagatgggc
      481 gaggagacgc gtcttgccgc tccccgcttc cccggcgagg aggatcacgt ccgggccgcg
      541 cgcgtcgccg atccgatcgg tgagtgagca gagcgtctcg tcgcggccct cgggcgctcc
      601 cgactcgagc cgggccgtcc gcgtcagatg ggtctcggcg ggaaagccgg tcccgacgag
      661 cgagttgagc ctcgcgagcg cctcggccgc gctcgcgggg cgtgcaccag gctcggtcgc
      721 tagcagcatc tcgagccaca ggcgcagtcc gtcgggaacg cggtcgggcc agacacgggg
      781 gcgacgctcc ccgctggtga cgcgagtgac gaagtcgccg atggcccggt cgtcctccag
      841 cgagaccggg ggctcgccga ggacgacttc gtggatcacg gcggcgagcc cgtacaggtc
      901 gctccggcgg ccggcaggtg ttccctggaa cagctcgggc gcgaggtagg gcaacgttcc
      961 ggccgggccg gcctcccggg tgccgtcgat cagcgccagt ccgaaatcga gcaacacgag
     1021 gcgcgagccg agacggggct cccggcgcac gagcaggttg gccggtttga catcgcgatg
     1081 gaagacggtg aaatcgtgta ggaaggccag tgcgcgcagc gcctcggcga tcagctcgag
     1141 cagcaccggc gccggcgcgc tgcggccgaa tgcggcgaga tcggtaccgt cgacgtattc
     1201 cagggtgaaa cagggactct caccgtcatg gaactcgtag acctcggcca ggttgggatg
     1261 gcgcagcgtg gccagcgcga agaactcgtc ccgcagggag cggctccgtg ccgtgtcacc
     1321 cccgccgagc gccttcagcg cgacgaggcg accaccgagc agtcggtcgc ggaccagcca
     1381 gacccgaccc gcgccgccct cgcccagcgg gcggatccgc tcgtagcgat cgagagacga
     1441 acgggccatg ggcagactat acgcccatgg cccgtgtttt ggagtgggaa gcgtctcaac
     1501 ttgagactct cggacccgtc agatcggaac gtggtcatct gtgatttccc gttccggttc
     1561 ttccggacgc ttccccagcg cgtgggcggc gaggctggtc cggtccggcc accagccgcc
     1621 gagatacgga agggaaccca gcgagtgaag ccggtccgcg ctggcgaagg cgaggcggaa
     1681 gaactgggca aaggtcgaga agcgaaagtc gaacggattc ggcgcctggg tcttcgtctt
     1741 gcccgtgaag gcgaagctcg tggccggccc ggggtcccgg gaagcggccg cgttcagccc
     1801 gccgacggag gtacacaaca gcaacgcgag cgcgaacacg atctgtcgac ggtgggtgcg
     1861 tctcatctct cgatctcctg ctgccatatt cgttttgcac gctaccaatg tgtttcaccg
     1921 tcataatcta ggtgaccagt aggagtgttc aatagtgaaa tcggtaatac taaatacgta
     1981 accaccgaca atccagacaa cggggagcga tcgagcgatc gccggctccg acgcgtcgtt
     2041 ttacgggact ttgacagggg cgagcgacgg gtggttgtat gttgtccgag gaaagccgtt
     2101 gaagatcctg gtggtcgagg acgagccgtc ccttcgcgag gggctgcggg acctgttgga
     2161 cggggccggc caccagaccc tgacggccga cgacggggac gcggggcttg ccgccgccgt
     2221 cgatccctcg atcgagctgg tcgttctcga cctgatgctg ccgcgggtcg acggcatcga
     2281 ggtttgccgg cggctccgcc gggctcgccc acggctgccg atcctggtgc tgaccgctcg
     2341 cggctcggag gaccaaaagg tctcggggct tcgcgccggg gccgacgatt acgtcaccaa
     2401 gccgttcggc acgcgcgaac tcctggcgcg gatcgaagcg ctggcgagac gggcgatcga
     2461 gcctcgggac ggcccgaccc agatcgcgac cgacggctgc cggatcgacc tcggtcgctg
     2521 tttggcgacc cgggacgggt ccgcagcggt acagctgacc gcgcgtgaga ccgagatcct
     2581 ccgccggctc tacgtccatc gaacgcgggc ggtcacgcga gccgagctgc tcgagaccgt
     2641 ttggggcgcc cggggtgacc tgcagacgcg caccgtcgac atgacgatcg ccaacctgcg
     2701 gcagaagatc gaacgggacc cggcgtcgcc gcggatcgtc gtcacggtca agggcgtcgg
     2761 ctacgcctgg ggcgagtcgt gatccgaccg ctcgcgggac gcaccgccgt ggcggcggtg
     2821 ctcacggccg cgggtgtcat cgtcccgtgc gtggcctggt acgtcgtcgg ctcccgcgcc
     2881 gcgatcgagc gcgcccaacg actcgccgag gagccgaacg tcgccgcgcg acgagaggcg
     2941 gcgcgactcg cccaacagct cgcccttcgg ctcgagtcga tgcgcgtgag cgaatcgcga
     3001 cgcccgttcc aggactatct gaccgccgat cggttgcgcg agctacaacg agattgctcc
     3061 tacgagatca cggtgccgtc gccgctggcc gaaggaccgg tcgacccgtt gatctgggct
     3121 cacttccagc tcgatgcggt cggcgtgctc acgctgccga cgttggcgga ggatggcccg
     3181 cgcggaggcg tcgacgacgc gagcgagatc cagcgggcga tcctcggcga gctggagtgc
     3241 gcgggcgcgg accacctgtc ggccttccac cggtcggcca gcccttccga gccgcgacaa
     3301 ctaccctcac ccctcgggtt gatcacggtc ggcccgtttg cttggcacac ggccacg
//
LOCUS       DASPGN010000257        10135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_747033, whole genome shotgun sequence.
ACCESSION   DASPGN010000257 DASPGN010000000
VERSION     DASPGN010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10135
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747033"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3520)
                     /locus_tag="VD788_07575"
     CDS             complement(<1..3520)
                     /locus_tag="VD788_07575"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXV76164.1"
                     /translation="MKLSRWSIWLVVVWSTTAAADGITVGGRVRVPGGLPLARADVQL
                     LVLAGSTADSRALFLGGPAEPVARVLSDEEGRFRIEAPHAGLWRVRVEAPGFVAMEHA
                     LEPLIEQLELPEVTLPADTGLKVSVRGPGGSPIAGAFVLAGPEHGPFATGGRSGWSAP
                     LRGGTTGAAGTVELPRGEGERIGVTASAAGHALAERRAISGTAVTLELLQAAGRPVVV
                     QAPDGSPIAAVLVVAGARHHPVAVTDEQGRAAVAVAGDGMIDARFVAEDGRQSGTRIG
                     GPPAADGKPRVVEIPPATLVAGRVIDAESRRSITGAVVYDAGTPTIAAVTDRAGSFVL
                     RAARSTRLTLTAGAPGYLAPDRPAEATLADDGRPGPTIALRPAAAIEGRVTAAAAGPL
                     AGVEVTIEEQQAQGTMRFEFGAPSAPPRASTDVEGRYRLGRVDPSKNWTVRARADGFA
                     PAEVRAAGLEPRELRTNVDLELRRGLSLIGSVTTLDSVPLADVEVTIEPSSSAARDGM
                     LGIRRETAPSRVVVATDGDGRFRADGLPAGKVDVTARRNGFAPGELRSVEIVAETTEP
                     VDAGGIVLEPGETIRGTVVNRDGAPIEGVEIRVEAAGGGGVMVILGAGMAADDEEPDA
                     LTDGSGWFELVDLSPKKRYTVSARRVGYLQASAGPFPLPHVEPVGLTLDPASDVRGRV
                     VDGAGEAIAGAEVAMTRNRTIEMGSNVMRTIMMQSETTDGDGRFVFEDQEPGEMSIKA
                     VASGYREAKLDGLSIAEGEDLEGVELTLAGGAVVTGQVLGPDGRPVIGAGVQPAGEGE
                     AMMRMLEGSPSDGNGIYRLEGLAPGAVSIEATHDDWPRTVRDVELTAGVNHLDLQFEG
                     GVEVEGRVSDTTGRGVAGAVVRLAPAGRGWGGPETIADGEGRYRMPGVQAGDYHVWAQ
                     APGYASYTSETEIEVRGEPLYAVDIELDTGGAIVGRVSGVEPERFSDVGVEAQGPSAR
                     GAGAVAVDGDGRYRVDNLGPGSYTVVASVANSGKRASGEVTLEPGAVEATLDLEFAPG
                     LTLSGRAVQAGSGIGGATVYVEGTNVVHDGWNETDHEGRFEIDGLEPGDYTIRLRHWK
                     TGLAYDEPLSLSSDKTITLDVPTARVAGRVVDAGDREPLGGVTLALTADGSTSTGASA
                     HSATTDVEGKFEFGNVADGAWTLSASK"
     gene            complement(3660..5303)
                     /locus_tag="VD788_07580"
     CDS             complement(3660..5303)
                     /locus_tag="VD788_07580"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXV76165.1"
                     /translation="MSLVVLAAAGPVVASEGPDLEVVHRIKQEAFKRSRVMETLHQMT
                     DVHGPRLTNSPGMDRAARWAAEQAAAWGLEQVAVESWGPFGRGWSASRFAAHLVDPQY
                     APLIGVPLPWAPGTGGPIRGTPLFAPLQDKTDHEDRVAELERYVEQWSGKLRGRMVLL
                     GEPPHIEPETDAPSERYSSSELSDRAEASDPVEPIEIDPTNVVLPDNPYERERFRAHA
                     PAWARAVLREQRRELRNRLNEFLVAEGVALVIRPARDGDAGTVFPSGAGSHRLDDPLP
                     PPSISLTPEHYNRIARLARNGGPVTIEVEVDARMHTDDLDGANVVAEIPGGSRADELV
                     IIGAHYDDWAYATGSTDNAAGCAVMMEAMRILAALDLPLARTARMVLWTGEEQGLLGS
                     KAYVRRHFGDPRTMELEPAHAKVSAYFNLDNGTGKIRGVYLQGNDMVRPIFKAWLAPF
                     GDHGASTVTIRDTGGTDHLSFDAVGIPGFQFIQDPVEYWSRTHHSNMDVYDRAVPADL
                     MQAAAIVATFVYQTANHDALLPRKPLPEPWPENLRHLDD"
     gene            complement(5624..6064)
                     /locus_tag="VD788_07585"
     CDS             complement(5624..6064)
                     /locus_tag="VD788_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76166.1"
                     /translation="MHGFRPMARAVAVVFAFAVAGNALAGDLDDLIGTINVSARADLG
                     GFKAELGATFGVSGTKIDGLFEVMSDPADVYMALRIGELTRQPVDRVLEQYRSNPNRG
                     WGAIAHDLGIKPGSEEFHALKQNRLSARANSGSGEKGAKGKGKG"
     gene            6382..6837
                     /locus_tag="VD788_07590"
     CDS             6382..6837
                     /locus_tag="VD788_07590"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXV76167.1"
                     /translation="MSSRLHDAEHLVRLGGLFTAGVLLFLLVRGLLIPDGFGAIGHYR
                     VGAIDDNRDQPPVYAGREACTDCHSEVVEAKTGGAHAAIGCEACHGPLAGHAGDPAGA
                     MPELPDPQRLCLGCHRSSVTKPEWFPQVAADEHAAGEPCGSCHLPHVPL"
     gene            6853..7635
                     /locus_tag="VD788_07595"
     CDS             6853..7635
                     /locus_tag="VD788_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXV76168.1"
                     /translation="MPTDRREFLRQGGKFVLLTSASAIGWEQLLAGHPERAPNYEMVE
                     HWWAMFIDIDKCIGCGNCVRACKVENDVPLEPFYFRTWVERYHIPPKSPADLTAEDRP
                     LVDSPNGGYDGFPERYSAGDGSKNFFVPKLCNHCAHSPCVQVCPVGATFESPDGVVLV
                     DKTYCVGCRYCVQACPYGCRYIDPRTHTVDKCDLCYHRITRGLTTACCEACPTGARLL
                     GDLKNPDDPIHRLLRAHKVQVLKPQMATGAKVYYNALDGSVR"
     gene            7644..8813
                     /gene="nrfD"
                     /locus_tag="VD788_07600"
     CDS             7644..8813
                     /gene="nrfD"
                     /locus_tag="VD788_07600"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXV76169.1"
                     /translation="MEQVIHGVEGFIYPNEIELQWSVLIVLYPYVTGLVAGAFILASL
                     ERVFNVDAVRPTYRLALLTALAFLLVAPLPLQLHLGHPERSFEMFLTPHTTSAMAMFG
                     FVYLWYLMAVLVIEIWLDYRKELVRTARASTGLKRLLYRGLTLGSDNLSERSLRIDDK
                     LGRIVTIVGIPSAFLLHGYVGFIFGSVKANPWWSTPLMPIVFLFSAIVSGIAAVLLVY
                     MVYTAMKGIPLEMRCLDKIGSYLFYAFVVDFSLELLDLIHRIYEADESFHSLDFMVHT
                     RLYNSHVILQIIIGTLLPIGMLAVTQVVRLSEAARKTFYAVSAVLALVGILAMRWNVV
                     IGGQLFSKSFLGYTTYKMGFATREGLLPAILVMVLPLVILWVLVKLLPPLEHETT"
     gene            complement(8820..>10135)
                     /locus_tag="VD788_07605"
     CDS             complement(8820..>10135)
                     /locus_tag="VD788_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HXV76170.1"
                     /translation="AVADYDGDGRSDILVTNFGPNVLYRNRGDGTFDNVAPGSPIEAP
                     GWNTGAAFFDADADGDLDLYVAAYIDQTIDAVIAAERVLDWKGVDRVAIGPFGLPGAV
                     DHFFLSDGAGRFVDSTTRAGLDDRAEAYGFAVRAADFDRDGDVDLYVANDSDANYLYR
                     NEGDAIFTEVGLWSGAALDANGAAQAGMGIAVGDPTGDGVYDLFVTNFSEDYSTLYRG
                     DGTGFFDDVSGPSGVGELTYDALSWGTVFADLDNDGDEDLVVANGHIYPQVDDHPQFG
                     LSYEQRNSLLENLGDGRFVDATARGGPGFEQLYSSRGLAAGDYDNDGDLDLLITHLDR
                     PPSLLRNDSTSGAWLIVDCRVPPGAGARTGTEVTVRFGGRIARRDLASGGSFLSVNDP
                     RVHFGLGTHERVERLEIRWPDGTLDRLDDVAARRIVSVTKSAEGR"
BASE COUNT         1444 a         3631 c         3494 g         1566 t
ORIGIN      
        1 gcttgctcgc gctgagcgtc caggcgccgt cggcaacgtt gccgaactcg aacttgccct
       61 cgacgtcggt cgtcgcgctg tgagcggacg ctcccgtcga cgtcgatccg tccgccgtca
      121 gcgccagcgt gacgccgccg agcggctcac ggtcgccggc atcgacgacg cgcccggcga
      181 cgcgcgcggt cggcacgtcg agcgtgatcg tcttgtcgga cgacaggctg agcggctcgt
      241 cgtacgccag cccggtcttc cagtgtcgca ggcggatcgt gtagtccccg ggctcgagcc
      301 cgtcgatctc gaagcggcct tcgtgatcgg tctcgttcca gccgtcgtgc acgacgttcg
      361 tgccctcgac gtagaccgtc gctccgccga tgcccgaccc ggcctgcacg gcgcggccgg
      421 acagcgtcag cccgggcgcg aactcgagat cgagcgtcgc ctcgacggcg ccgggctcga
      481 gcgtgacctc gccgctcgcg cgtttgcccg agttggccac gctggcgacg acggtgtagc
      541 tccccggtcc caggttgtcc acccggtagc gaccgtcgcc gtcgacggcg acggcgccgg
      601 cgccgcgagc gctcgggccc tgcgcctcga cgccgacgtc ggagaatcgc tcgggctcga
      661 cgccgctgac gcgaccgacg atcgcgccgc ccgtgtcgag ctcgatgtcg acggcgtaga
      721 gcggctcgcc gcggacctcg atctcggtct cgctcgtgta gctggcgtag cccggcgcct
      781 gggcccacac gtggtagtcg ccggcctgga ccccgggcat gcggtagcgg ccctcgccgt
      841 cggcgatcgt ctccggaccg ccccagcccc gtccggcggg cgccagtcgg acgaccgcac
      901 cggcgacgcc gcggcccgtc gtatcgctga cccgtccttc gacctcgacg ccgccctcga
      961 actggagatc gaggtggttg acgccggccg tcagctccac gtcgcgaacc gttcgcggcc
     1021 agtcgtcgtg ggtcgcctcg atcgagaccg ctcccggtgc gagcccctcg agacgataga
     1081 tcccgttgcc gtcggacggc gagccttcga gcatcctcat catcgcttcg ccctcgcccg
     1141 cgggctgcac gccggcgccg atcaccggac gcccgtcggg gccgagcact tggccggtga
     1201 cgacggcacc gccggcgagc gtcagctcga cgccctcgag gtcctcgccc tcggcgatgc
     1261 tcagcccgtc gagcttggct tcgcggtagc ccgaggcgac ggccttgatc gacatctcgc
     1321 cgggctcctg gtcctcgaag acgaagcgcc cgtcgccgtc ggtcgtctcg gactgcatca
     1381 tgatcgtgcg catcacgttg ctgcccattt cgatcgtgcg gttgcgcgtc atcgcgacct
     1441 cggcaccggc gatcgcctcg ccggccccgt cgaccacccg gccgcgaacg tcggacgccg
     1501 ggtcgagcgt caacccgacc ggttccacgt gcggcagcgg aaacggcccg gcgctcgcct
     1561 gcaggtagcc cacgcgtcgg gcggagacgg tgtagcgctt cttcggggag aggtcgacga
     1621 gctcgaacca gccggagccg tcggtcagcg cgtccggctc ctcgtcgtcg gccgccatgc
     1681 cggcgccgag tatcaccatc acacccccgc ccccggccgc ctcgacgcgg atttcgaccc
     1741 cttcgatcgg cgcgccgtcg cgattgacga ccgtgccgcg gatcgtctcg ccgggttcga
     1801 gcacgatgcc cccggcgtcg accggttcgg tcgtctcggc gacgatctcg accgaccgca
     1861 gctcacccgg cgcgaacccg ttccgccggg cggtgacgtc gaccttgccg gccggcaggc
     1921 cgtcggcgcg gaaccgccca tcgccgtccg tcgcgacgac gacgcgcgac ggtgccgttt
     1981 ctcggcggat gccgagcatc ccgtcgcgcg cggcggagga ggacggctcg atcgtgacct
     2041 cgacgtcggc gagcgggacg ctgtcgagcg tggtgacgct gccgatcagc gacaagccgc
     2101 ggcggagctc gaggtcgacg ttcgtgcgca gctcgcgcgg ctcgagaccc gccgcccgca
     2161 cctcggccgg tgcgaagccg tcggcacgag cccggaccgt ccagttcttg ctcgggtcga
     2221 cccgcccgag gcggtagcgt ccctcgacgt cggtcgacgc gcgcggcggc gcgctcggcg
     2281 ccccgaactc gaagcgcatc gttccctgcg cctgctgctc ctcgatcgtg acttcgacac
     2341 cggcgagcgg gccggcggcc gccgccgtta cccgaccctc gatcgcggcc gcgggtcgca
     2401 gcgcgatcgt cggtccgggc cggccgtcgt cggcgagcgt cgcctcggcc ggtcggtccg
     2461 gggcgaggta tcccggcgca ccggcggtca gggtgaggcg cgtcgaccgt gccgcgcgga
     2521 gcacgaacga gccggcgcga tcggtgaccg ccgcgatcgt cggcgtgccg gcatcgtaga
     2581 ccaccgcgcc ggtgatcgac cggcgcgact cggcgtcgat cacccgaccg gcgacgaggg
     2641 tcgcgggcgg aatctcgacg acgcggggct tgccgtcggc cgccggcggc ccgccgatgc
     2701 gcgtgccgga ttggcgtccg tcctcggcga cgaaccgcgc gtcgatcatc ccgtcgcccg
     2761 cgacggcgac ggcggcgcga ccttgctcgt cggtcaccgc gaccgggtga tgacgggcac
     2821 cggcgacgac cagcacggcc gcaatcggcg atccgtcggg cgcttgcacg acgaccggcc
     2881 ggccggccgc ctgcagcagc tcgagcgtca ccgccgtacc ggagatcgcc cgtcgctcgg
     2941 ccagggcgtg gcccgcggcc gacgcggtca cgccgatgcg ctcgccctcg ccgcgcggca
     3001 gctcgaccgt gccggcggct ccggtcgtcc caccccgcag cggggcgctc caaccgctcc
     3061 gcccgccggt ggcgaacggc ccgtgctccg ggccggcgag cacgaaggcg ccggcgatcg
     3121 gcgagccgcc gggcccgcgc acgctgacct tgagcccggt gtcggccggc agcgtgacct
     3181 ccggcagctc gagctgctcg atcaacggtt cgagcgcgtg ctccatcgcg acgaagccgg
     3241 gcgcctcgac gcgcacgcgc cacagaccgg cgtggggcgc ttcgatgcgg aaccgaccct
     3301 cctcgtcgct cagcacgcgc gcgacgggct cggcgggccc gccgaggaac agcgcgcggc
     3361 tgtcggccgt cgatccggcc agcaccagca gctgcacgtc ggcccgcgcg agcgggaggc
     3421 cgccgggcac gcgcacgcgt ccgccgacgg tgatcccgtc cgccgccgcc gtcgtgctcc
     3481 aaacgaccac caaccagatc gaccacctgc tgagcttcat tccaccctcc gcatcgatcc
     3541 cgggtccgcc gcttgcaagt gcccggccag cgaaatcctc gcgtccgcgc gggccgaccg
     3601 cgccgcgccg cccgaaatga gggaggtcta cccgatttga ggcaagcgcc gccggccgat
     3661 cagtcgtcga ggtgccgcag gttctcgggc cagggctccg gcagcggttt gcgcggcaac
     3721 aacgcgtcgt gattcgcggt ttggtagacg aaggtggcga cgatcgcggc ggcctgcatc
     3781 aggtcggccg ggacggcccg atcgtagacg tccatgttcg agtgatgcgt tcgcgaccag
     3841 tactcgacgg gatcctggat gaactggaat cccgggatgc cgacggcgtc gaacgacagg
     3901 tggtcggtcc cgcccgtgtc gcggatcgtc accgtcgacg caccgtgatc gccgaagggc
     3961 gcgagccagg ccttgaagat cgggcggacc atgtcgttgc cctgcaggta gacgccgcgg
     4021 atcttgccgg tgccgttgtc cagattgaag taggccgaaa ccttggcgtg tgcgggctcg
     4081 agctccatcg tccgtggatc accgaagtgc cggcgcacgt aggccttcga gccgagcaac
     4141 ccctgctcct ccccggtcca cagcaccatc cgcgccgtgc gcgcgagggg aagatcgagc
     4201 gctgcgagga tccgcatcgc ctccatcatc acggcgcaac cggcggcatt gtcggtgctg
     4261 ccggtcgcgt aggcccaatc gtcgtagtgc gcaccgatga tcaccagctc gtcggcgcgc
     4321 gagccgccgg ggatctcggc gacgacgttc gcaccatcga ggtcgtcggt gtgcatccgc
     4381 gcgtcgacct cgacctcgat cgtcaccgga cccccgtttc gcgccagccg cgcgatgcgg
     4441 ttgtagtgct cgggcgtcaa cgagatcgac ggcggcggca gggggtcgtc gagacggtgc
     4501 gagccggcgc cggacgggaa aacggtcccg gcgtcgccat cccgcgcggg acggatcacg
     4561 agtgccaccc cttcggcgac gaggaactcg ttcaaccggt tgcgcagctc gcggcgctgc
     4621 tcccgcagca ccgcacgcgc ccaggccggg gcgtgtgcac ggaagcgctc gcgctcgtac
     4681 gggttgtccg gtaggacgac gttcgtcgga tcgatctcga tcggctcgac cgggtcgctc
     4741 gcctcggccc gatcgctcag ctccgagctg ctatagcgct cggacggggc gtcggtctcc
     4801 ggctcgatgt ggggcggctc cccgagcagc accatcctgc cgcgcagctt cccggaccac
     4861 tgctcgacgt agcgctcgag ctccgccacg cggtcctcgt gatcggtctt gtcctgcagc
     4921 ggagcgaaca gcggcgtacc gcggatcgga ccgccggtgc cgggggccca cgggagaggc
     4981 acgccgatca acggagcgta ctgcggatcg accaggtgcg cggcgaatcg gctggcggac
     5041 cacccgcgac cgaacggccc ccagctctcc acggcgacct gttcgagacc ccaggcggcg
     5101 gcctgctcgg cggcccagcg cgccgcgcgg tccatcccgg gggagttcgt cagacgcggc
     5161 ccgtggacgt ccgtcatctg atgcagcgtc tccatcaccc gcgagcgctt gaacgcctct
     5221 tgcttgatgc gatggacgac ctcgaggtcg gggccctcgg acgcgacgac cgggcccgcc
     5281 gccgccagga cgacgagcga caacctaccg actccggatt cgatcaaccg ggtgcccccg
     5341 agcgacgctc gcgccgcgcg ggcgcgccgg gcgagtatgg ggggcggacg ggatgctgtc
     5401 aacgcggccg ggccggtaca aaaaacgcgg aaggcggcga ccacaccact agacaccgcc
     5461 ttccgcgtac cgaggccccc gggagggacg acggccgatc gcccgtcgga cgggctgaat
     5521 cctacgccgc ccagcagctg gccgacgttg aggacccgcc gcgactccgg ctcacggggt
     5581 gggacagtgg cggcgagcgg ccggaacagg tttttatcgg actctagccc ttccccttgc
     5641 ccttcgcgcc cttctcgccc gagccgctat tcgcgcgcgc cgacaggcga ttctgcttga
     5701 gtgcgtggaa ctcctcggaa ccgggcttga tgccgaggtc gtgggcgatc gcgccccacc
     5761 cgcgattcgg gttcgagcga tactgctcga ggacgcgatc gaccggctgg cgtgtcagtt
     5821 caccgatccg cagggccatg tagacgtcgg ccgggtcgga catcacctcg aacaaaccgt
     5881 cgatcttggt ccccgagacg ccgaaggtcg cgccgagctc ggccttgaac ccgccgaggt
     5941 ccgcgcgtgc gctcacgttg atcgtcccga tcagatcgtc gagatcgccg gccagcgcgt
     6001 tccccgcgac ggcgaaggcg aagacgacgg cgaccgctcg agccatcggt cgaaaaccgt
     6061 gcatcggtcc tccttttcgg acgatgggcg tccgaccgag gcaatctggt tccgcgaggg
     6121 gggagatgca acgcggggcg ggggcgacgc gcccgcgtga gccgcggacg ccccgtcgag
     6181 gccgccagtc tgccagatcg cgctcggagg cggcgcgatt cgtgcggtcc gccggtggcg
     6241 cgagcgcgaa cccgaccggt tcggtcctca atcgcgggtc gaaccatcgg gcgtgctgat
     6301 gtagaaaccg actgtgacga aacggccgcg agggggcggg atgccgggaa gcgtcgggct
     6361 gtcgatcgcc gcggtgggcg gatgagttcc cggctgcacg atgccgagca cctcgtgcgg
     6421 ctcggcgggt tgttcaccgc cggcgttctg ctgttcctgc tggtgcgcgg gctcttgatc
     6481 ccggacggct tcggcgcgat cggtcactac cgggtcggcg cgatcgacga caaccgcgat
     6541 caaccgccgg tctacgccgg tcgcgaggcg tgcaccgact gccactcgga ggtcgtcgaa
     6601 gccaagaccg gcggcgcgca cgcggcgatc ggttgcgagg cgtgccacgg cccgctcgcc
     6661 ggtcacgccg gcgacccggc gggcgcgatg ccggagctgc ccgacccgca gcggttgtgc
     6721 ctgggttgcc accggtcgag cgtgaccaag cccgagtggt tcccacaggt cgccgccgac
     6781 gagcacgccg cgggcgagcc gtgtgggtcg tgtcacctac ctcacgtccc gctctgagac
     6841 gaagggggat cgatgccgac cgaccgacgc gagttcctca ggcaaggcgg caagttcgtc
     6901 ctgctcacct ccgcctcggc gatcggttgg gagcaactgc tcgccggtca tccggagcgt
     6961 gccccgaact acgagatggt cgagcactgg tgggcgatgt tcatcgacat cgacaagtgc
     7021 atcgggtgcg gcaactgcgt gcgggcgtgc aaagtcgaga acgacgtgcc gctcgagccc
     7081 ttctacttcc gcacgtgggt cgagcgctac cacatcccgc ccaaatcgcc cgccgatctc
     7141 accgccgagg accgcccgct ggtcgattcc cccaacggcg gctacgacgg cttccccgag
     7201 cggtactccg ccggtgacgg ctcgaagaac ttcttcgttc ccaagctgtg caaccactgc
     7261 gcgcactcgc cgtgcgtcca ggtctgtccg gtcggggcga cgttcgagag tccggacggc
     7321 gtcgtgctgg tcgacaaaac ctactgcgtc ggctgccgtt actgcgtgca ggcctgtccc
     7381 tacggctgcc gttacatcga cccacgcacc cataccgtcg acaagtgcga cctgtgctac
     7441 caccgcatca cgcgcggcct gacgaccgcc tgctgcgagg cctgcccgac gggggcgcgc
     7501 ctgctcggcg acctgaagaa ccccgacgac ccgatccacc gcttgctgcg ggcgcacaag
     7561 gtgcaggtgc tcaagccgca gatggcgacc ggcgccaagg tctactacaa cgcgctcgac
     7621 gggtcggtac gctaggagga gtgatggagc aggtcattca cggcgtcgaa gggttcattt
     7681 accccaacga gatcgagctg cagtggagtg tgctgatcgt gctctacccg tacgtaaccg
     7741 ggctggtcgc cggcgccttc atcctcgcct cgctcgaacg ggtgttcaac gtcgacgcgg
     7801 tcaggccgac ctaccggctg gcattgctga ccgcgttggc cttcctgttg gtcgcgccgc
     7861 tgccgctgca gttgcacctg gggcaccccg agcgatcgtt cgagatgttc ctcacgccgc
     7921 acaccacgtc ggcgatggcg atgttcggct tcgtctacct gtggtacctg atggcggtgc
     7981 tggtgatcga gatctggctc gactaccgca aggagctggt gcggacggca cgagccagca
     8041 ccggcctcaa gcggctgctc taccgtggcc tgacgctcgg ctcggacaac ctcagcgaac
     8101 gctcgctgag gatcgacgac aagctgggcc ggatcgtcac gatcgtcggc atcccgtcgg
     8161 cgttcctgct gcacggctat gtcggcttca tcttcggctc ggtcaaggcc aacccgtggt
     8221 ggtcgacccc gttgatgccg atcgtcttcc tgttctccgc catcgtctcc ggcatcgccg
     8281 ccgtgctgct ggtctacatg gtctataccg cgatgaaggg cattccgctg gagatgcggt
     8341 gcctggacaa gatcgggagc tatctgttct acgcgttcgt cgtcgacttc tcgttggagc
     8401 tgctcgatct gatccaccgg atctacgagg ccgacgagtc gttccacagc ctcgatttca
     8461 tggtccatac ccggctctac aactcccacg tgatcctgca gatcatcatc ggcacgctgc
     8521 tgccgatcgg catgctggct gtcacgcagg tcgtgcggct gagcgaggcg gcgcgcaaga
     8581 cgttctacgc cgtctcggcc gtactggcgc tggtcggcat cctggcgatg cgctggaacg
     8641 tcgtgatcgg cggacagttg ttctcgaaga gctttctcgg ctacacgacc tacaagatgg
     8701 gctttgccac gcgcgagggg ttgctgcccg cgatcctcgt gatggtgctg ccgctcgtca
     8761 tcctgtgggt gctggtcaag ctgttgccgc cgctcgagca cgagacgacg tagtcgccgt
     8821 taccgtccct ccgcgctctt cgtcacgctg acgatccgcc gcgcggcgac atcgtcgagc
     8881 cggtcgagcg tgccgtcggg ccagcggatc tcgagacgct cgacccgctc gtgggtgccc
     8941 agaccgaagt gcacccgcgg gtcgttgacc gacaggaacg acccgccgct cgcgagatcg
     9001 cgccgagcga ttcgaccgcc gaaacggacg gtgacctcgg ttccggtgcg agcgccggct
     9061 ccgggcggca cgcggcaatc gacgatcagc cacgcgcccg aggtcgaatc gttgcgcagc
     9121 agcgacgggg gccggtcgag gtgggtgatc agcaggtcga gatcgccgtc gttgtcgtag
     9181 tcaccggcag ccaggccacg gctcgagtag agctgctcga agcccggacc gccacgcgcg
     9241 gtggcgtcga cgaagcggcc gtcgccgagg ttctcgagca acgagtttcg ttgctcgtag
     9301 ctcagcccga actgcggatg atcgtcgacc tgcgggtaga tgtggccgtt ggcgacgacg
     9361 aggtcttcgt cgccgtcgtt gtcgaggtcg gcgaagacgg tgccccagga caacgcgtcg
     9421 taggtcagct cgccgacgcc ggacgggccc gagacgtcgt cgaagaagcc ggtgccgtcg
     9481 ccgcggtaga gcgtgctgta gtcctccgag aaattggtta cgaacaggtc gtacaccccg
     9541 tcgccggtgg ggtcgccgac cgcgatcccc atccccgcct gcgcggcgcc gttggcgtcg
     9601 agcgcggcgc cggaccacag gccgacctcg gtgaagatcg cgtcgccttc gttccggtag
     9661 aggtagttgg cgtccgagtc gttcgcgacg tacagatcga cgtcaccgtc gcggtcgaag
     9721 tcggcggccc gaaccgcgaa accgtaggcc tcggcgcggt cgtccagacc agcccgggtg
     9781 gtcgaatcga cgaagcggcc tgccccgtcg gagagaaaga agtggtcgac agccccggga
     9841 agcccgaacg gcccgatcgc cacccgatcg actcccttcc agtcgagaac ccgctcggcc
     9901 gcgatcaccg cgtcgatcgt ctggtcgatg taggccgcga cgtacagatc gaggtcgccg
     9961 tcggcgtcgg catcgaagaa cgcggcgccg gtgttccagc ccggcgcttc gatcggcgat
    10021 cccggcgcga cgttgtcgaa cgtcccatcg ccccggttgc ggtagagcac gttgggcccg
    10081 aagttcgtca ccaggatgtc gctgcggccg tcgccgtcgt agtccgcgac ggcca
//
LOCUS       DASPGN010000258         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1449173, whole genome shotgun sequence.
ACCESSION   DASPGN010000258 DASPGN010000000
VERSION     DASPGN010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449173"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /gene="ispH"
                     /locus_tag="VD788_07610"
     CDS             complement(<1..986)
                     /gene="ispH"
                     /locus_tag="VD788_07610"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HXV76171.1"
                     /translation="MVEKLYLAKPRGFCAGVVMAIEAVERSAEQMRRDGLGELSVYHA
                     IVHNDTVVGRLAHEHGVHFVEDLDELARLEDRIERRGGRLSDTVVFSAHGVSPAVLRR
                     ADELGLHTIDATCPLVTKVHSEAKKYARLGYHILLIGDSTTHQEVIGTRGEAPRQTTV
                     VSVVGNRKHDPGLADAESVQVPDPSKVVVLTQTTLSVDDTARTIDILRRRFPRLVVPS
                     RDDLCYATKNRQDAVRKIAERVDLFLVVTSDYSSNGMRLLELAESLTGNARRIESVAD
                     LDEQWFDGVTSVGVTSAASTPDDLVQQIVAHFRAVNPDLDVVEQGEWEEIEFR"
     gene            1255..2208
                     /locus_tag="VD788_07615"
     CDS             1255..2208
                     /locus_tag="VD788_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV76172.1"
                     /translation="MSTAIVESTSSSPVTIRAEGLRHVYKGGTVALDGVDLEFGTGLF
                     GLLGPNGAGKSTLMRIMCTLLVPTAGRVTVGGHDVCRERLAVRRLLGYLPQEFGGWRL
                     QRVEEVLDTLAILSGMRDRSRRRGRIAEVLRQVGLDEVADRKVKKLSGGMVRRLGVAQ
                     ALVHEPRVLIVDEPTVGLDPQERLRFRQLMASLGQDRTIVLSTHIVADLGSSCRDLAL
                     IDGGKIVFRGSPEELMSQARGRVFQLAAGGADLAAVEQRYEVVSSVRDGEVNRVRAVS
                     RDGRLPDGAQPVDDPTLEEAYLAFMAARGRSDALSAEESER"
     gene            2205..>3796
                     /locus_tag="VD788_07620"
     CDS             2205..>3796
                     /locus_tag="VD788_07620"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV76173.1"
                     /translation="MNRLSPIWALARAESRLAFRLVRYWVFLVLATLFGAAQWGQFYL
                     IHHLFSAGSASAASANPRYFIGNFGGFLMLIFVVGLIFLAFDVRARDRRERIDEVVDT
                     TPVSNLEFVLGRALGILLAVWLPMSLITLALAGVVWAVGAPIEPVSLAAFLFLMMIPG
                     FLFSIGLVFLVTIVVRTRLLAAIASAILLTLLFVAGIWWIPFYLTPLTDMTGGYSVSF
                     PSDLIPSLSTLGGWAQRSGYALMGLAMLWLTAAMHPRRDDSRRSRLAAGGALMLAAGL
                     ALCAWQVLDWRSTFRQRESWRAAHQERAGEPLPSIRAITGEVRIDPGRSLAMQLELRV
                     TPQGDEPLESALFSLNPGLTVERVAADGVEAGFTQEDGLLDVALASPLVPGTERSLSL
                     SIHGVPDGDFAYLDAPLDILTLNPAEAQIFILGFEPLIFDRRYVALMPGSRWLPAAGT
                     EIGRGPAATRPRDFFDVDLTLTLPDGWRVAGPGRDIGDGGSHRFRPPAPVPQVALIGA
                     RFESWSTEIDGVKLELLLHPSHA"
BASE COUNT          511 a         1303 c         1325 g          657 t
ORIGIN      
        1 cggaactcga tctcttccca ctcgccctgc tcgaccacgt cgaggtcggg gttgaccgcg
       61 cggaagtggg cgacgatctg ctggacgaga tcgtccggcg tcgacgcggc cgacgtgacg
      121 ccgacgctcg tcacgccgtc gaaccactgc tcgtccagat cggcgaccga ctcgatgcgg
      181 cgggcgttgc cggtcagcga ctcggccagc tcgagcagcc gcatgccgtt gctgctgtaa
      241 tcgctcgtca ccaccaagaa caggtcgacc cgttccgcga tcttgcgcac ggcgtcctga
      301 cgattcttgg tcgcgtaaca cagatcgtct cgcgacggca cgaccagccg cggaaagcgc
      361 cggcgcagga tgtcgatcgt cctggcggtg tcgtcgacgc tgagcgtggt ctgggtcaac
      421 acgacgacct tcgacgggtc cggcacctgc accgattcgg cgtcggccag gcccgggtcg
      481 tgtttccggt tgccgactac gctgaccacg gtcgtctgac gcggtgcctc gccacgggtc
      541 ccgatcacct cctggtgcgt cgtcgagtcg ccgatcaaca ggatgtgata gcccagccgc
      601 gcgtatttct tcgcttcgct gtgcaccttg gtcaccagcg ggcaggtcgc gtcgatcgtg
      661 tgcaggccga gctcgtcggc ccgccggagg accgccgggc tgacgccgtg cgccgagaag
      721 acgacggtgt cggacagtct gccgccgcgc cgttcgatcc gatcctcgag ccgcgccagc
      781 tcgtcgagat cctcgacgaa atggacgccg tgctcgtgcg ccagccggcc gacgacggtg
      841 tcgttgtgga cgatggcgtg gtacacgctc agctcgccga ggccgtcgcg ccgcatctgc
      901 tcggccgagc gctcgacggc ctcgatcgcc atgacgacgc cggcgcagaa gccgcggggc
      961 ttcgccaaat agagcttttc gaccatcgcg tgcgattata gagggatccg ccgcggaacg
     1021 cttacggcgc gtcgggccgg ggatgcgctg gcgcacgccg agcacaaaac ccgtgcgtgc
     1081 ggcaaccgtc ggcattcgac cggggccgac cggaaggcag cgccggccgc gcccgccggg
     1141 ggaccgtcca ctcgcgaaaa cggcccgctc gcgatccaca tcccaccgaa cgcggcgatc
     1201 ggggcgggcg gcggtctata gtgtgggccg aaacggcact cgaggggatc cgaaatgagc
     1261 acagcgatcg tcgaatccac atcatcctcg ccggtcacga tccgggccga gggcctgcgt
     1321 cacgtctaca aaggcggcac cgtcgcgctc gacggggtcg acctggagtt cggcaccggg
     1381 ctgttcggcc tgctcggtcc gaacggcgcg ggaaagagca cgttgatgcg catcatgtgc
     1441 acgctgctcg taccgaccgc gggccgcgtc accgtcggcg gacacgacgt ctgccgcgaa
     1501 cggctcgcgg tgcggcgcct gttgggctat ctgccgcagg agttcggtgg ctggcgcttg
     1561 cagcgcgtcg aagaggtact cgacacgctg gcgatcttgt cgggtatgcg cgaccgctcc
     1621 cgacggcgcg gccgcatcgc cgaagtgttg cgtcaggtcg gtctcgacga ggtcgcggac
     1681 cgcaaggtga agaagctgtc cggcggcatg gtgcgccgcc tcggcgtggc gcaggcgctg
     1741 gtccacgagc cgcgggtgct gatcgtcgac gagccgaccg tcggtctcga cccgcaggag
     1801 cggctgcgat tccgccagtt gatggcgagc ctgggccagg accgcacgat cgtgctgtcg
     1861 acgcacatcg tcgccgacct cggctcgagc tgtcgcgacc tggcgctgat cgatggcggg
     1921 aagatcgtct tccgcggcag ccccgaggag ttgatgtcac aggcgcgggg acgcgtcttc
     1981 caactggccg cgggcggtgc cgacctcgcc gcggtcgaac agcggtacga ggtggtgtcg
     2041 agcgtgcgcg acggcgaggt caaccgggtt cgcgccgtca gccgcgacgg ccggttgccc
     2101 gacggggctc agcccgtcga cgacccgacg ctcgaggaag cctacctggc cttcatggcc
     2161 gcgcgcggac ggagcgatgc gttgtcggcc gaggagtcgg agcgatgaac cggctgtcac
     2221 cgatctgggc gctggcgcgc gccgaatcgc gcctggcatt ccgcctggtg cgctactggg
     2281 tgtttctcgt gctggcgacc ctgttcggcg ccgcccagtg gggccagttc tacctcattc
     2341 accatctgtt ctcggccggc agcgcgtcgg cggcgtcggc caacccgcgc tacttcatcg
     2401 ggaacttcgg cggcttcttg atgctgatct tcgtcgtcgg gctgatcttc ctcgcgttcg
     2461 acgttcgcgc gcgcgacagg cgcgaacgga tcgacgaggt ggtcgacacg acaccggtct
     2521 cgaacctcga gttcgttctc ggccgggcgc tcggcatcct gctcgccgtc tggctgccga
     2581 tgtcgttgat caccctcgcg ctggccgggg tcgtctgggc cgtcggcgca ccgatcgagc
     2641 cggtctcgct ggccgcgttc ctgttcctga tgatgatccc gggcttcctg ttctcgatcg
     2701 gtctggtctt cctcgttacg atcgtcgtgc gcacccgact gctcgcggcg atcgcgagcg
     2761 ccatcctgtt gacgctgttg ttcgtcgccg gcatctggtg gattccgttc tatctcaccc
     2821 cgctgaccga catgaccggc ggttactcgg tcagtttccc ctccgatctg atcccgtcgc
     2881 tcagcacgtt gggcggatgg gcgcagcgtt cgggctacgc gttgatgggg ctggcgatgc
     2941 tgtggctgac cgccgcgatg cacccgcggc gcgacgacag ccggcggtcc aggctggccg
     3001 ccggcggggc gctgatgctg gcggccggtc tcgcgctctg cgcgtggcag gtgctcgatt
     3061 ggcgctcgac cttccgccag cgcgagagct ggcgcgccgc gcaccaagag cgcgcaggcg
     3121 agccgctgcc ctcgatccgg gcgatcaccg gcgaggtgcg tatcgatccc gggcgctcgc
     3181 tggcgatgca gctcgagctg cgggtgacgc cgcaggggga cgagccgctc gagtcggcgt
     3241 tgttctcgct gaatccaggg ctgaccgtcg agcgcgtcgc cgccgacggg gtcgaggccg
     3301 gcttcaccca ggaggacggc ttgctcgacg tcgcgctggc gtcgccgctc gttcccggta
     3361 cggagcggtc gctgtcgctg tcgatccacg gcgttcccga cggagacttc gcctatctcg
     3421 acgcgccgct cgacattctc acgctcaacc cggccgaagc ccagatcttc attctcggat
     3481 tcgagccgtt gatcttcgac cggcggtacg ttgcgctgat gcccggctcg cgctggctgc
     3541 cggccgccgg caccgagatc ggtcgcggcc cggccgccac gcgcccgcgc gacttcttcg
     3601 acgtcgatct gaccctcacg ctgccggacg gctggcgcgt ggccggcccg gggcgagaca
     3661 tcggcgacgg cggctcgcac cgctttcgac cgccggcacc ggtcccgcag gtcgcgttga
     3721 tcggcgcgcg gttcgagtcg tggtcgacgg agatcgacgg cgtcaagctc gagctgttgt
     3781 tgcacccgag ccatgc
//
LOCUS       DASPGN010000259         2651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_395985, whole genome shotgun sequence.
ACCESSION   DASPGN010000259 DASPGN010000000
VERSION     DASPGN010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395985"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..2211)
                     /gene="ispG"
                     /locus_tag="VD788_07625"
     CDS             complement(124..2211)
                     /gene="ispG"
                     /locus_tag="VD788_07625"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HXV76174.1"
                     /translation="MEPWSYDGLTYRRWPSRTVRVGEVEIGSRHPIRVQSMTTPSTKN
                     TRATVDQIVRLVDAGCEIVRVTVPTSSDADALPAIRAALARAGLRVPLVADIHFTPAA
                     ALRAVEHVEKIRINPGNFADKKKFAVREYTDAQYRAELERIAQTFSPLVERAKELGVA
                     MRIGTNHGSLSDRIMNRYGDTPQGMVESALEFVDICRDHGYHDLVLSMKASNPVVVHH
                     VYRLLVVRMSELGMDYPIHLGVTEAGDGEDGRIKSALGIGTLLEEGIGDTVRVSLTED
                     PVAEIPVARRLVERYNRLRDDPQAAPPGLVPSLRGEVREQRDPHVYRRRPGRETRVGP
                     RAVGGANPVRVEIPLEGRLGEGDALRRELDRHAGVRVPEETRAELVSVAVADETELAA
                     LAGLRRSLELVAPQVALSARIGFDWRRISSESCVGRWVEACHRLELVVGSPLEPGDLA
                     LFERLLRRAASASTALLFEVSGADAETAVEVAVALASLDRRLGTRCALLGLAPIAGAS
                     SLQPTRLLAARLSAERLEPSVVLIDDARRRGDDCLLGAAVGIGGSLCDGFGDSVQLRG
                     QPNGDSRKIAYGILQAARVRITKTEFISCPSCGRTLFDLEETTARIKQRTAHLKGVKI
                     AVMGCIVNGPGEMADADFGYVGWGDDKIALFVGREMVARDVPTAEADSRLVELIKQHG
                     RWVDPPVPVAEDA"
     gene            2318..2644
                     /locus_tag="VD788_07630"
     CDS             2318..2644
                     /locus_tag="VD788_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76175.1"
                     /translation="MKEIEPLAKRGHYVVAAVVSALGVVVCLGALAGFILTVATNRLR
                     WYPGLTIQEHYLAIGRSYTQGFVVGFFLCFFMVVGALALSRFVERYRRRSRHAVRASS
                     SGARVA"
BASE COUNT          396 a          927 c          918 g          410 t
ORIGIN      
        1 gggaggcgac cgacgggccg tgaggtcccg gccggcgagg gacggacggg ccgtcgggaa
       61 accgggccgg cggggccgag cggcaatccg cggtcggggg cgacgaaccg ggagagccgg
      121 tgttcaggcg tcctcggcga ctggaacggg gggatcgacc cagcgaccgt gctgcttgat
      181 cagctcgacg aggcgggagt ccgcctcggc ggtcgggacg tcgcgtgcga ccatctcccg
      241 gccgacgaac agcgcgatct tgtcgtctcc ccagccgacg tagccgaagt cggcgtcggc
      301 catttcgccc ggcccgttga cgatgcagcc catcaccgcg atcttgaccc ccttgaggtg
      361 cgccgtgcgc tgcttgatcc gtgcggtcgt ctcttcgaga tcgaacagcg ttcggccgca
      421 ggacgggcag gagatgaact cggtcttggt gatgcgcacc cgagcggcct gcaggattcc
      481 gtaagcgatc ttccgggaat ccccgttcgg ttgaccgcgc agctgcacgc tgtcgccgaa
      541 tccgtcgcac aacgaaccgc cgatcccgac cgccgcaccg agcaggcagt cgtcgccgcg
      601 acggcgcgcg tcgtcaatca gcacgaccga tggttcgagc cgttcggcgc tcaagcgcgc
      661 ggcgagcagc cgggtcggtt gcagcgacga cgctccggcg atcggcgcga gccccaagag
      721 cgcgcaacgg gtgccgagcc ggcgatcgag tgacgccagc gcgacggcca cctcgactgc
      781 cgtctcggca tccgccccgg atacctcgaa caagagcgcg gtggatgcgg acgcggcccg
      841 gcgcaacagc cgctcgaaga gggcgagatc gccgggctcg agcgggctgc cgacgacgag
      901 ctcgagccga tggcacgcct cgacccagcg gccgacgcac gactcggagc tgatccggcg
      961 ccagtcgaaa ccgatccgag ccgacagcgc cacctggggt gcgacgagct ccagcgatcg
     1021 tctcagcccg gcgagggcgg cgagctccgt ctcgtccgcg acggcgaccg acacgagctc
     1081 ggcccgggtc tcctcgggga cccgcacacc ggcgtggcga tcgagctcgc ggcgcaacgc
     1141 gtcaccctcg ccgagacggc cctcgagcgg aatctcgacc cgcaccggat tcgccccgcc
     1201 gacggcacgc gggccgaccc gggtctctcg gccgggtcgc cgtcgataga cgtggggatc
     1261 ccgctgctcc cgcacctcgc cgcgcaacga cgggaccagt cccggcggcg cggcctgcgg
     1321 gtcgtcgcgc aaccggttgt aacgctcgac gagccggcga gcgacgggga tctccgccac
     1381 cggatcctcg gtcagcgaga cgcggacggt gtcgccgatg ccctcctcga gcagcgtgcc
     1441 gatcccgagc gccgatttga tccggccgtc ttcaccgtca ccggcctccg tcacaccgag
     1501 gtggatcgga tagtccatgc cgagctccga catccggacg accagcagcc ggtagacgtg
     1561 gtgcacgacg accggattgc tggccttcat cgacagcacc aagtcgtggt agccgtggtc
     1621 gcgacagatg tcgacgaact ccagggcgga ttcgaccatg ccctgcggcg tgtcgccgta
     1681 gcgattcatg atgcggtccg acagcgagcc gtgattcgtg ccgatccgca tcgccacgcc
     1741 cagctccttg gcccgctcga ccagcggcga gaaggtttgg gcgatccgtt cgagctcggc
     1801 ccggtactgc gcgtcggtgt actcgcgcac ggcgaacttc ttcttgtcgg cgaagttgcc
     1861 gggattgatc cggatcttct cgacgtgctc gacggcccgc agggccgcgg cgggcgtgaa
     1921 gtggatgtcg gcgacgagcg gcacgcggag gccggcccgc gccagcgccg cgcggatcgc
     1981 ggggagcgcg tcggcgtcgc tcgacgtcgg cacggtgacg cgcacgatct cgcaacccgc
     2041 gtcgacgagc cgcacgatct gatcgacggt cgcccgcgtg ttcttcgtcg agggcgtcgt
     2101 catcgattgg acgcggatcg gatgtcggct gccgatctcg acttcgccga cgcggacggt
     2161 cctgctgggc cagcgacggt aggtcaaccc gtcatagctc cacggttcca tctggcattc
     2221 atcctcttac ggccgcgcgt gggcagacgg cgctagtcta gcagctgacc aattttggca
     2281 ctggcgtcac ctgcgtcggg ccaggatatt gatcgggatg aaggagatcg agcccctggc
     2341 gaaacgcggc cactacgtcg tggcagccgt cgtttcggcg ctcggagtcg tcgtctgcct
     2401 cggagcgctc gcggggttca tcctcaccgt cgcgaccaac aggctccgct ggtacccggg
     2461 gctcacgata caggagcact atctcgcgat cggccggtcc tacacgcagg gcttcgtcgt
     2521 cggttttttt ctttgttttt tcatggtcgt cggcgcactc gccctctccc gcttcgtcga
     2581 gcgctaccgg cggcggtcgc ggcacgcggt acgggcgtca tcctccggcg cccgcgtcgc
     2641 ctgagcccgc t
//
LOCUS       DASPGN010000260         9149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_981040, whole genome shotgun sequence.
ACCESSION   DASPGN010000260 DASPGN010000000
VERSION     DASPGN010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9149
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_981040"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VD788_07635"
     CDS             <1..1068
                     /locus_tag="VD788_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV76176.1"
                     /translation="RARNDALEKTREFLSRIIEGSAEAIVTLDRHSRIASWNRAAEQI
                     YGWTAEEMIGAGLERLLPAGEATAAEQARIEAEVRGGRTVRDSDSVHVRRDGSPVTVR
                     MTVSPLYAENGEHEGSTAIIRDVTSLVDLERRVREQDRLAAVGRLAAQVAHEIKNPLA
                     GIRGACEVMMTRLEQDSGKEIAAEVVRQIDRLNRTVEDLLLFSRRSSLRPVPSDLHAV
                     IDSVIGVVQGAADARRLEVVRDFSPRIGNVTVDPEQIQQVLFNIIINAAQVMDYAGQM
                     TIRTRLVDHSVVVCVRDSGPGIPPECLTQVFEPFYTTRAQGNGLGLAIVKKIVHSHDG
                     TVEARNHPELGAEFEIRLPYR"
     gene            1132..2241
                     /locus_tag="VD788_07640"
     CDS             1132..2241
                     /locus_tag="VD788_07640"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXV76177.1"
                     /translation="MTRRISRRALLRGAAGSLLLAASTRAAPREPTEIRVLTFNAWHG
                     LRGAPRLRLDGEDPRRKARRFDWQIEQIRRLDPDLLLLQEVNKNARETRRYADALAYD
                     HINKVTNCGIHLPPVKIPTNFNDGLAILARPGLGLRRVGHKRLSGDAICGESIGFQTR
                     ESRFVLFGEIRLGSRTVLVATTHLSAPPPLADPFFARLDRLVAQGRVAHEQRETIARR
                     VDRARRRNLAEVETIVRALDGYRRPKETAGRYRHLVVGGDFNAEPDTAAIATLERAGL
                     RDVSSDRGLLTWDPRRNRENQSIGRRRSPPVPTFGNPAIEALVGDGTGRPRQIDYIFV
                     SSEFTAVSVELALERPLDGLYASDHFGVFSVLRLG"
     gene            2292..3212
                     /gene="miaA"
                     /locus_tag="VD788_07645"
     CDS             2292..3212
                     /gene="miaA"
                     /locus_tag="VD788_07645"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXV76178.1"
                     /translation="MPARLLVIAGPTASGKTRLGVRVAHALGSEIVSADSRQIYRGLD
                     LGSGKDLDEYAAVRPPVRHHLIDVVDPRSVYSVFQFQADCFRLLETKAAEPPFDRGVP
                     LLMVGGSGLYVEAVLRRYRIPAVPADEGLRRRLMAREHGELIEALEHADPGLARRTDL
                     SSKKRVVRALEIATHRFDRVPRASPTHPPIDFLVFAIDIPRATLDQRIDARLDDRLRR
                     GLVDEVRGLLDDGVSPARMARLGLEYREVTAFLTGHKSERQMIDDLRRGIRRLAKRQL
                     TWFRGMPRRGIELQWIGPDEVERVARHPWV"
     gene            complement(3209..4210)
                     /gene="trpS"
                     /locus_tag="VD788_07650"
     CDS             complement(3209..4210)
                     /gene="trpS"
                     /locus_tag="VD788_07650"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXV76179.1"
                     /translation="MGGKRVFSGIQPTGNLHLGNYLGAIKQWVARQDEKESFICIVDL
                     HAITIHQHPAELLENTRALAAMLLACGIDPQRTTLFVQSHVRAHAEASWLLSCITPLG
                     WLERMTQFKDKSARQQTIQTGLLTYPVLQAADILLYDADEVPVGEDQKQHIELSRDIA
                     QRFNSLYGETFKLPTAVIPQVAARVMGLDDPTLKMSKSSTAQGHAVRLTDDDEQILKA
                     FKRAVTDSGREIAFSGAPDRAGVNNLLGIYRALTDKTAAQVLADFRDARGYGDLKLRV
                     AEAVVERFRPIRRRYLELLEQPAELDRLVAIGAQRAAARAEPKVIEMKRRMGLLPAG"
     gene            complement(4222..4794)
                     /locus_tag="VD788_07655"
     CDS             complement(4222..4794)
                     /locus_tag="VD788_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HXV76180.1"
                     /translation="MSKLWVLFLVLCAVVALAGCGSEQSGSAEPEEAATEPVQPSIQR
                     IDAVLSAKSGSELTGVAIFVKDAAGVSLQLNVENTPPGEHAVHIHEFGDCSADDASSA
                     GGHWNPSTEDHGRWGMPPHHLGDIGNITVGEDGTGSLSLSTDRWTMGDGSVTDIVGKS
                     IIVHADPDDFTSQPTGAAGGRIGCAVIETY"
     gene            4867..5148
                     /locus_tag="VD788_07660"
     CDS             4867..5148
                     /locus_tag="VD788_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76181.1"
                     /translation="MVYNLPMIELLSAAVSYIGMLCILSAFVLETRGALSSRGGVYLW
                     LMAIGSALLALRAAHSREWAFLVLELVWSIAALLALVRVSRASATGQGA"
     gene            5171..7729
                     /locus_tag="VD788_07665"
     CDS             5171..7729
                     /locus_tag="VD788_07665"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXV76182.1"
                     /translation="MKAASGPELPARLVVCVVYVVFFLSGACTLVYELVWTRMLVLVF
                     GTSVLAVSTVLAAFMAGLALGAWWFGRRIDRHDNGLRVYAYLEFGVAVCAVLFPLLLS
                     RLDGLYTEIYRALGGNDAVFALIRFSLLFVVLLVPTTLMGGTLPVLAKHVVRRLDALG
                     RGVGLLYAVNTLGAVAGCAATAYLLIEAAGTRGATHLAAAVNLLIGVAALVRARATPD
                     TPGASAGAPRPAARARASIALGGLRRLVLWGLAFSGFAALGYEVLWTRLLTMVLHITT
                     VQSLSTILIVFLTGLALGGAAATRFVDRLPHLPTVFGGLQLLLGLFGLSSVALLGSIP
                     SWLPLLDGFPSWSGHMFKLFAVSFAVMIGPTFVMGMLFPLAARIYIDGLDGLGARVGQ
                     LYAANTGGAILGAFLTGFALIPAIGTQFSMWTLASINLAIGIAVPAADAWPRRKVKLA
                     LAAIVAIGVVLLIAALPADQLANLFHGAQPDGTLLHFDEALGGTVTVHAMPSGIRLLR
                     VNGAGEVPTDRASIRTFRLLGSLPMLLHPDAQQVLIVAYGGGVTLAAVEMHGPRTIDC
                     VEVMPRVVEASRLFGQYNDRVFDRIGQGAIRLIADDGRNHILRTERRYDVIVSDSTHP
                     STADSWILYTEEFYRLCRARLNEGGIVAQWLPLHGLSAADHQMILRTFRAVFPYATLW
                     LTRGYGVLLATPEPLTIDFDRLQRRLSAPRVARSLQEVDLDDPISFLAALALDERAFA
                     EYAGSGPLNTDDRPYLSFDDRTRAGTGTGVFVLYEILPHLVEQLPRPPDGIGNDGLAR
                     LGLRFRARRHVFAADVALRIGNRERAIEELRRARTIDPTGREAERMLRSLEGGG"
     gene            7729..8784
                     /locus_tag="VD788_07670"
     CDS             7729..8784
                     /locus_tag="VD788_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76183.1"
                     /translation="MRRLHLAGGDSVVGRLAASGVDGEFLCWSDVLHEGPVPGGLSPE
                     QLNTVRATFLAQRYASGSLDDVRRALQARLARLAGAADDDEVTLWFAHDLADQLRLLQ
                     LLDWFARDRATAPKLTLVSIDAHPEVARFRELAQLTPRQLSALDWSRRRVHDGDFELA
                     RRAWAAFTAAEPTGLVELGRDRCRALPFLSRALHRHLQQFPGSSDGLSRTEREILDHV
                     ALRGGTVREAFEVTQNEYEEAPFLGPATFLHYVDRLLVGPEPLLDFAVGFRDSRWFHR
                     LRLTAHGRETLAGRRQRHVRGGVDRWLGGVHLDGPTPAWVWDAASQSLIAGVRHPPPD
                     TRPDRRGDPPSLGRDSG"
     gene            8787..>9149
                     /locus_tag="VD788_07675"
     CDS             8787..>9149
                     /locus_tag="VD788_07675"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HXV76184.1"
                     /translation="MRRAPPHPFFEVPHPIVLGHRGAAGEAPENTLAGFARALELGAT
                     VIEADVRLTADAVPVMIHDSTIDRTTNGRGTVAAMTFDGLQRLDAGFSFSADGGASYP
                     FRGCGVRVPSLAQALAEFA"
BASE COUNT         1253 a         3183 c         3175 g         1538 t
ORIGIN      
        1 cgcgcgcgca acgacgcgct cgagaagacc cgcgagttcc tcagccgcat catcgaagga
       61 tcggccgagg cgatcgtcac gctcgatcgt cactcgcgga tcgcgtcgtg gaaccgggcc
      121 gccgagcaga tctacggctg gaccgcggag gagatgatcg gcgcgggtct cgagcggctg
      181 ttgcccgcgg gcgaggcgac cgccgccgag caggcgcgca tcgaggccga ggtgcggggg
      241 ggccgcacgg tgcgggattc cgacagcgtg cacgtccggc gcgacggctc gccggtcacc
      301 gttcgcatga ccgtctcgcc gctctacgcc gagaacggcg agcacgaggg gtcgaccgcg
      361 atcatccgcg acgtcacctc gctcgtcgat ctcgaacggc gggtgcgcga acaggaccgg
      421 ctggcggccg tcggcaggct ggccgcgcag gtggcccacg agatcaagaa cccgctggcc
      481 gggattcgcg gcgcctgcga agtgatgatg acccgtctcg agcaggattc gggcaaggag
      541 atcgcggccg aggtcgtgcg tcagatcgac cggctgaacc gcaccgtcga ggacctgctg
      601 ctgttctcgc gacgttcgtc gttgcggccg gttccgtccg acctgcacgc ggtgatcgat
      661 tcggtgatcg gcgtcgtgca gggggcggcc gacgcgcggc ggctcgaggt ggtccgcgat
      721 ttttctcccc ggatcggcaa cgtcaccgtc gacccggagc agatccagca ggtgctgttc
      781 aacatcatca tcaacgcggc acaggtgatg gactacgcgg gccagatgac gatccggacc
      841 cggctggtcg accacagcgt ggtcgtttgc gttcgtgaca gcggaccggg catcccgccc
      901 gaatgcctga cccaggtgtt cgagccgttc tacaccaccc gcgcacaggg caacgggctg
      961 gggctggcga tcgtcaagaa gatcgtccac tcccacgacg ggacggtcga ggcgcgcaat
     1021 catccggagc tcggcgcgga attcgagatc cggctgccgt accgctgacc cgcggcgccg
     1081 gcgccccact tcggccggag cccgcgccgg attgaattac catgagcctc gatgacgcgc
     1141 cgcatctccc gaagagcgct gcttcgcggc gccgccggct cgttgctgct cgccgcctcg
     1201 acccgcgcgg cgccgcgcga gccgaccgag atccgcgtgc tgaccttcaa cgcctggcac
     1261 ggtctgcgcg gagcaccgcg gctgcgactc gacggcgagg acccacggcg caaggcgcgg
     1321 cgcttcgact ggcagatcga gcagatccga cggctcgacc ccgatctgtt gctgctgcaa
     1381 gaggtcaaca agaacgcgcg ggagacgcgg cgctacgccg acgcgctggc ctacgaccac
     1441 atcaacaagg tgacgaactg cgggatccac ctgccaccgg tcaagattcc gacgaacttc
     1501 aacgacgggt tggcgatcct ggcccggccc ggcctcggtc tgcgacgcgt gggccacaag
     1561 cgactgagcg gggacgcgat ctgcggcgag tcgatcggct tccagacgcg agaatcgcgt
     1621 ttcgtgttgt tcggcgagat acggctcggc tctcgcacgg tgctcgtcgc cacgacgcat
     1681 ctgtcggcgc cgcccccgct ggccgacccg ttcttcgcgc gactcgaccg gctcgtcgcg
     1741 cagggacggg tcgcgcacga acagcgcgag acgatcgcgc ggcgggtcga tcgcgcgcgg
     1801 cggcggaacc tcgcggaagt cgagacgatc gtccgggcgc tcgacggcta ccggcgaccg
     1861 aaggagaccg ccggtcgcta tcgccacctg gtcgtcggcg gagacttcaa cgcggagccc
     1921 gacacggcag cgatcgcgac gctcgagcgg gcgggcctgc gcgacgtgtc gtccgatcga
     1981 ggcttgctca cgtgggatcc gcggcggaac cgtgagaacc agtcgatcgg ccggcggcgc
     2041 tcgccgccgg tgccgacctt cggcaacccg gcgatcgaag cgctggtcgg ggacgggacg
     2101 ggccgaccac ggcagatcga ctacatcttc gtctcctccg agttcaccgc ggtcagcgtc
     2161 gagctggcgc tggagcggcc gctggacggc ctttacgcgt cggaccactt cggtgtcttc
     2221 tccgtgctga ggctcggttg agacgggcgg cgacctcgaa caatggttcc gcgcgcgctt
     2281 cggaggctga cttgcccgcg cgactgttgg tcatcgccgg cccgacggcc agcggcaaga
     2341 cccggctcgg cgttcgggtg gctcacgctc tcggttccga aatcgtctcg gccgactcgc
     2401 gccagatcta ccgcggtctc gatctgggtt cgggcaagga tctcgacgag tacgccgcgg
     2461 tccgaccgcc cgttcggcac cacctgatcg acgtggtcga tccacgcagc gtctactccg
     2521 tcttccagtt ccaagccgat tgcttccgtc tgctcgagac caaggccgcg gagccgccgt
     2581 tcgatcgcgg cgtgccgctg ttgatggtcg gcggaagcgg cctctacgtc gaggccgtgc
     2641 tgcggcgtta tcgcatcccg gccgttcccg cggacgaggg gctgcgccgt cgcttgatgg
     2701 cgcgggagca cggcgagctg atcgaagcgc tcgagcacgc cgatccgggg ctggcgcggc
     2761 gcaccgacct gagcagcaag aagcgggtgg tgcgcgcgct cgagatcgcc acacaccggt
     2821 tcgaccgagt cccgcgggcc agcccgaccc atccaccgat cgattttctc gtcttcgcca
     2881 tcgacatccc gcgcgccacg ctcgaccaac gcatcgatgc gcgcctcgac gaccgactcc
     2941 ggcgcggctt ggtcgacgag gtgcgaggcc tgctcgacga cggcgtctcg cccgcgcgga
     3001 tggcccggct gggtctcgag tatcgcgagg tgacggcctt cctcaccggc cacaagagcg
     3061 agcggcagat gatcgacgac ctgcgccgcg gcatccgccg cctggccaag cgtcagctga
     3121 cctggttccg cggcatgccc cgccgcggga tcgagctgca gtggatcgga ccggacgagg
     3181 tcgagcgcgt cgcgcggcac ccgtgggtct agcccgcggg caacagcccc atcctgcgct
     3241 tcatctcgat gaccttcggc tcggctcgtg ccgcggcgcg ttgggctccg atcgcgacca
     3301 gccggtcgag ctccgccggc tgttcgagta gctcgaggta tcgccgtctg atcggccgga
     3361 agcgctcgac caccgcttcg gcgacgcgca gcttgaggtc gccgtagccc cgcgcatcgc
     3421 ggaaatcggc gaggacctgc gcggcggtct tgtcggtcaa cgcgcgatag atgccgagca
     3481 ggttgttcac gcccgcacgg tcgggggcgc cggagaacgc gatctcgcga ccggagtcgg
     3541 tcacagcccg tttgaacgcc ttgaggatct gttcgtcgtc gtcggtgagg cggaccgcgt
     3601 gaccctgtgc ggtcgagctc ttcgacatct tcagtgtcgg gtcgtcgaga cccatcacgc
     3661 gggccgcgac ctgggggatc accgcggtgg gaagcttgaa cgtctcgccg tacagactgt
     3721 tgaaccgttg ggcgatgtcg cgagacagct cgatgtgctg cttctggtcc tcaccgaccg
     3781 gcacctcgtc cgcgtcgtaa agcaggatgt cggcggcctg cagcaccggg taggtcaaca
     3841 gaccggtctg gatcgtctgc tggcgcgccg acttgtcctt gaactgggtc atccgctcga
     3901 gccagccgag cggggtgatg cagctcaaca accagctcgc ctcggcgtgc gcgcgaacgt
     3961 ggctctgcac gaacaacgtc gtccgctgtg gatcgatgcc gcaggcgagg agcatggcgg
     4021 ccagagcgcg cgtgttctcc agcagctccg ccggatgctg gtgaatcgtg atcgcgtgca
     4081 ggtcgacgat gcagatgaag ctctctttct cgtcctgccg cgcgacccac tgcttgatcg
     4141 caccgaggta gttgccgagg tgcaagttgc cggtcggctg gataccggag aatacgcgct
     4201 tgccccccat ccgccgtccg ctcagtaggt ctcgatcacg gcgcagccga tccgcccacc
     4261 ggcggctccg gtcggctgcg aggtgaagtc gtccgggtcc gcgtgcacga tgatcgactt
     4321 gccgacgatg tcggtcaccg acccgtcgcc catcgtccag cgatcggtgc tcagcgacag
     4381 cgaaccggta ccgtcctcac cgacggtgat gttgccgatg tcgcccaggt ggtgcggcgg
     4441 catgccccac ctgccgtggt cctcggtcga cgggttccaa tgaccaccgg cggaactggc
     4501 gtcgtcggca ctgcagtcgc cgaactcgtg gatgtggacc gcatgttcgc cgggcggggt
     4561 gttctcgacg ttcagctgga gcgagactcc ggccgcgtcc ttgacgaaaa tggcgacgcc
     4621 ggtgagctca ctgccgctct tggccgacag cacggcgtcg atccgttgga tcgacggttg
     4681 gaccggctcg gtcgcggcct cctccggctc ggcacttccc gattgctcgc ttccgcatcc
     4741 cgcaagcgcg acgacggcgc acagcaccaa gaacaggacc cacagcttgc tcatggcttc
     4801 ctccgttcga tcatccccga cgcaggctag cggtttcttg cgggttggtc aacgcgcacg
     4861 gcgcgggtgg tttacaatct gccgatgatc gagctgctct ccgccgcggt gtcgtacatc
     4921 ggaatgctct gcatcctgtc ggcgttcgtt ctcgagaccc gcggcgcgct gtccagtcgc
     4981 ggcggcgttt acctgtggct gatggcgatc ggctcggccc tgctcgcgtt gcgcgccgcc
     5041 cactcgcgcg agtgggcgtt cctggttctc gagctggtct ggtccatcgc cgccctgctc
     5101 gcgctcgtcc gcgtgagtcg cgcgagcgcg accggccagg gggcgtgatg accacgcgac
     5161 gcgtggccgc gtgaaggctg cttcgggtcc cgagctgccg gcgcgtctcg tcgtctgcgt
     5221 cgtctacgtc gtcttctttc tgtcgggcgc ctgcacgctg gtctacgagc tcgtctggac
     5281 ccgcatgctc gtgctcgtct tcggcaccag tgtgctggcg gtcagcacgg tgctcgccgc
     5341 cttcatggcg ggtttggccc tcggcgcctg gtggttcggc cgccggatcg atcgccacga
     5401 caacggactg cgggtctacg cttatctcga gttcggcgtc gcggtctgcg ccgtgctgtt
     5461 cccgctgcta ctgtcacggc tcgacggcct ctacaccgag atctaccgcg cgttgggcgg
     5521 caacgacgcg gtgttcgccc tgatccgctt cagcctgctg ttcgtcgtgc tgctcgtgcc
     5581 gacgacgctg atgggcggaa cgctgccggt gctcgccaag cacgtcgtgc gacggctcga
     5641 cgccctcggc cggggcgtgg gtctgctcta cgcggtcaac acgctcggcg cggtcgccgg
     5701 ctgcgccgcc accgcctacc tgttgatcga ggccgccggc acgcgcggcg cgacacacct
     5761 ggccgccgcg gtcaacctgc tgatcggcgt ggcggcgctc gtgcgggccc gcgccacgcc
     5821 cgatacgccc ggcgcgagcg ccggtgcgcc ccgccccgcg gcgcgagcgc gagcgtcgat
     5881 cgcactggga ggcctgcgcc ggttggtgtt gtggggtctg gcgttctccg ggttcgcggc
     5941 gctgggctac gaggtgctgt ggacccggct gctgacgatg gtgctgcaca tcacgaccgt
     6001 gcagagcctg agcacgatcc tgatcgtatt cctgaccggt ctggcgctgg gcggcgcggc
     6061 ggcgacacgg ttcgtcgatc ggctgccgca cctgccgacc gttttcggcg ggctgcagct
     6121 gctgctcggt ctgttcgggc tcagctcggt cgcgctgctc ggcagcattc cctcgtggtt
     6181 gccgctgctc gacggcttcc cgtcgtggtc ggggcacatg ttcaagctgt tcgcggtctc
     6241 gttcgccgtg atgatcggtc ccacgttcgt catgggaatg ctgttcccgc ttgccgcccg
     6301 gatctacatc gacggtctcg acggcctggg tgcccgcgtc ggacagctct acgccgccaa
     6361 cacgggcggg gcgatcctcg gcgcgttcct caccggattc gcgttgatcc cggcgatcgg
     6421 tacgcagttc agcatgtgga cgctggcgtc gatcaacctg gcgatcggca tcgcggtccc
     6481 ggccgccgat gcctggccgc gccgtaaggt caagctggcc ctcgccgcga tcgtggcgat
     6541 cggcgtcgtc ctgctgatcg cggcgctgcc ggccgatcaa ctcgcgaatc tgttccacgg
     6601 cgcgcagccc gacgggacgc tgctccattt cgacgaggcg ctcggcggca ccgtcacggt
     6661 ccacgcgatg ccgagcggca tccgcttact gcgggtcaac ggcgcgggcg aggtcccgac
     6721 cgaccgtgcg tcgatccgca ccttccgcct gctcggctcg ctgccgatgc tgctgcaccc
     6781 cgacgcgcag caggtgctga tcgtcgccta cggcggcggg gtgacgttgg cggcggtcga
     6841 gatgcacggc ccgcgcacga tcgactgcgt agaggtgatg ccgcgtgtcg tcgaggcgtc
     6901 gcggttgttc gggcagtaca acgatcgcgt gttcgaccgc atcggccagg gcgccatccg
     6961 gctgatcgcc gacgacgggc gcaaccacat cctgcgcacc gagcggcgct acgacgtgat
     7021 cgtcagcgac tcgacccacc cgagcacggc cgacagctgg atcctctaca ccgaagagtt
     7081 ctaccggctc tgccgcgccc ggttgaacga gggcggcatc gtcgcccagt ggttgccgtt
     7141 gcacggcctg tcggcggccg accaccagat gatcctgcgt acgttccgcg cggtttttcc
     7201 atacgcgacg ctgtggctga ctcggggcta cggcgtgctg ctggcgacgc ccgagccgct
     7261 gacgatcgac ttcgatcgtc tgcagcgccg cctgtcggct ccgcgggtgg cgcgcagcct
     7321 gcaggaggtc gacctcgacg acccgatctc gttcctcgcc gcgctggcgt tggacgagcg
     7381 agcgttcgcc gagtacgcgg gcagcgggcc gctcaacacc gacgatcgtc cctacctcag
     7441 cttcgacgac cgcacgcgcg ccggaacggg gacgggcgtc ttcgtgctgt acgagatact
     7501 gccgcacctc gtcgagcaac tgccgcgccc gccggacggc atcggcaacg acgggctggc
     7561 tcgcctcggg ctgcggtttc gcgcgcgacg ccacgtattc gccgccgacg tcgcgctgcg
     7621 catcggcaat cgcgagcgcg cgatcgaaga gctgcgacgc gcgcgcacga tcgatccgac
     7681 gggacgggag gccgaacgga tgctccgatc gctagagggc ggtggctgat gcggcgcctc
     7741 cacctggccg gcggcgattc ggtcgtcggc cgactggccg cgagcggagt ggacggcgag
     7801 ttcctctgct ggtccgacgt cttgcacgaa ggcccggtgc cgggtggcct atctcccgag
     7861 caactgaaca cggtgcgcgc gacgttcttg gcgcagcgtt acgcgtcagg ctcgctcgac
     7921 gacgtccgtc gagcgttgca ggcgcgcctc gcgcgcctcg ccggggcggc cgacgacgac
     7981 gaggtgacgt tgtggttcgc ccacgatctc gccgaccagc tgcggctgct ccagctgctc
     8041 gactggttcg cacgggaccg ggcgaccgcg ccgaagctga cattggtctc gatcgacgct
     8101 catccggagg tcgcgcgttt tcgcgagctg gcccagctca ccccgcggca actgtccgcg
     8161 ctcgattgga gccgacgacg cgtgcacgac ggagatttcg agctcgcccg ccgcgcctgg
     8221 gcggcgttta ccgccgccga acccaccggg ctcgtcgagc tcggccgcga tcgatgtcgg
     8281 gcactgccgt tcctgtcgcg ggcgctgcac cgccacctgc agcagtttcc cggctccagc
     8341 gacggtttgt cgcgcaccga gcgcgagatc ctcgatcacg tcgcgctgcg cggcgggacg
     8401 gtgcgcgagg cgttcgaggt gacgcagaac gagtacgagg aggcgccgtt cctcggcccg
     8461 gcgacgttcc tgcactacgt ggaccgactg ctggtcggcc ccgaaccgct gctcgacttc
     8521 gcggtcggct tccgtgacag ccggtggttc caccggctgc gcctcacggc ccacggccgg
     8581 gagacgctcg ccggccggcg gcagcgtcac gtccgcggcg gcgtcgaccg ctggctcggc
     8641 ggtgtgcacc tcgacggccc gacaccggcc tgggtctggg acgccgcctc gcagtcattg
     8701 atcgccggcg tgcgtcaccc gccgccggac acgcgaccgg accggcgcgg cgatcctcca
     8761 tcgctcggtc gagacagcgg ttgacggtgc gccgcgctcc gccccatccg ttcttcgagg
     8821 tcccgcatcc gatcgtgctc ggtcaccgcg gggcggcagg cgaggcaccg gagaatacgc
     8881 tcgcggggtt cgctcgagcg ctcgagctgg gcgcgacggt tatcgaggcc gacgtgcggt
     8941 tgaccgccga cgcggtgccg gtgatgatcc acgattcgac gatcgatcgc acgacgaacg
     9001 gtcggggtac ggtcgccgcg atgaccttcg acgggctgca acgactcgac gccgggttct
     9061 cgttctccgc ggacggcggg gcgagctacc cctttcgcgg ctgcggcgtg cgcgtgccct
     9121 cgctggccca ggcgttggcg gagtttgcc
//
LOCUS       DASPGN010000261         4525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1449591, whole genome shotgun sequence.
ACCESSION   DASPGN010000261 DASPGN010000000
VERSION     DASPGN010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449591"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VD788_07680"
     CDS             <1..989
                     /locus_tag="VD788_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HXV76185.1"
                     /translation="VVSVILDDRDAVADGHRLNARFAAQIPEQLTTGLIGLATSRAWN
                     TVTLFDKQSGRVHGVVPTGRAASGVALDRQRLRAYVAAPGADTVATIDLLSNRIIDRL
                     QMQVGDRPTELVLIAGGAVVLTANAGSNTVSFVDTQRLFEIDRLPVGTGPAAIALDPE
                     RSRAYVFNRLGDTISLIDLTARRVVLTAATDAAPVRGAFDRRGATLFVIHASSPYLTL
                     FNPDLTIRSRVFVGPGASALTVDQRTDRVYLARRGTDSIDVYDPSSLLPIDAIPTDGD
                     VSFLAIDGELNNLAVLTIETRGIRLINLTNRDPVAEIDLGPEPYWVTFLGEG"
     gene            1062..3209
                     /locus_tag="VD788_07685"
     CDS             1062..3209
                     /locus_tag="VD788_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76186.1"
                     /translation="MTVVATAWAVTSRSSARDLSGFVEYAYGEGTDRRTDPPAPEVER
                     ESSFFGQRYNVVWNRQIYENLRLLTGGYFEGLDQQQQLGGLVTDSSITTFRPFVNLLL
                     SSPVWSSELGWDRNEQSTDPGVGAGLTFVRDNYRATFGWEPDRYPLVQLRLLRTDDRD
                     RQRSLRDRDFRTVQLISEYNPRDPVYLYYTGAWRRTEDAINDNTVTDVSHTGRFVYND
                     SYLNRRALINSDWTGTYRTTDTTTSGTGEISLPVFPFEGLSEIDEFPEQGPLARNAAL
                     IDDDFDTSAGIDLGLPPPAGDDRSRNFGLDFADTVEINTLFVWIDRDIPLDTAASFSW
                     RIYTSDDNQEWTLRDSVPAAVFGGAFLPRFEIRFDNVEARYVKLVTEPLQSTDPDAGD
                     FPSIFVTELEAFIRSPAADVEGTRSLFSQIYNLNFRYRITDAPRLFYELGYFLTDTDA
                     GPSSWVLSNGFSVFQRFARIYTLNARYAREEIRDSSVERDANQLTGSLSADPLETVRF
                     SLVYSGREEDSFFGTTEGQSLFLLTVLELYRGVSLNAGFGRSYREIDPGRLTESRQAN
                     GTLTMQPHRTLAMTLFWQDRRDDLSGGGLTDERRVTRSTELNAAWRPVQALYLFGAFR
                     VEDRPDVDARTTSRYLFSWAPFRDGTLSLVLNYNETLRSDFDTRETVSSSRIRWQVSG
                     NTFVNLGFAKITQESDLFDSDSSNLSADVRFGF"
     gene            3234..3818
                     /locus_tag="VD788_07690"
     CDS             3234..3818
                     /locus_tag="VD788_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76187.1"
                     /translation="MSRIARTIRLAPVVVLLLGAACAHKTQKVTFHDPNMDFGLLRSV
                     AVMPFGNETADRSAADRVRDVFMTTLQATGAFYVLPPGEVARGISRVSPEDETAPTPD
                     NVVALANTLEVDAVITGVVLEYGEVRSGTASANVITVSLRMLEAQSGRVVWSASTTKG
                     GVSASDRLFGGGGQPMNLVTQRAIDELLDSLFSG"
     gene            3835..>4525
                     /locus_tag="VD788_07695"
     CDS             3835..>4525
                     /locus_tag="VD788_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76188.1"
                     /translation="MIRDRSSRVARALCTVAVSLVAARAIAAQTPVPVGREARIAVLP
                     MVNLSAKRAPMDELRALLGDHADLRGLETLRPAELETFMSSRRMRYTGGVDSATAVAL
                     RESTGAQAVLISALHSYDEADPPTFAIVARLVTTESDPRIVWMAEVSLTGDQTPGLLA
                     LGLVRDVATLRDRAFEQLFDSLVEQLGSAEPPRVGRLKAKNRFRPKRFYRTPQRPESD
                     DAAVRVAVLPFI"
BASE COUNT          722 a         1538 c         1493 g          772 t
ORIGIN      
        1 aggtcgtctc ggtcattctg gacgatcgcg acgcggtcgc cgacggtcat cgactgaacg
       61 cccgcttcgc cgcccagatc cccgagcaac tgacgaccgg cctgatcggc ctcgccacca
      121 gccgcgcgtg gaataccgtc accctgttcg acaagcagag cggtcgcgtc cacggcgtcg
      181 tgccgacggg ccgggcggca tcgggggtcg cactcgaccg ccagcggttg cgggcctacg
      241 tcgccgcgcc cggcgccgac accgtcgcca cgatcgacct gctgtcgaac cgcatcatcg
      301 accgactgca aatgcaggtc ggcgatcggc cgaccgagtt ggtgctgatc gcgggcggcg
      361 ccgtcgtgct gaccgcgaac gccggctcga acacggtcag cttcgtcgat acgcagcgat
      421 tgttcgagat cgatcggctc ccggtcggca ccggaccggc cgcgatcgcg ctcgacccgg
      481 agcgaagccg cgcctacgtc ttcaaccgcc tcggcgatac gatctcgctg atcgatctga
      541 cggcgcgtcg ggtcgtgctg acggccgcga ccgacgcggc accggtgcgc ggcgcgttcg
      601 accgccgcgg ggcgacgctg ttcgtgatcc acgcatcgtc tccgtacctc accctgttca
      661 acccggatct gacgatccgc agtcgggtct tcgtcggccc gggtgcgtcg gcgttgaccg
      721 tcgaccaacg caccgatcgc gtctacctgg cccgtcgcgg aaccgattcg atcgatgtct
      781 acgatccgtc ttcgttgctg ccgatcgacg ccattccgac cgatggggat gtgtcgttcc
      841 tcgcgatcga cggcgaactg aacaacctgg cggtgctgac gatcgagacg cgagggatcc
      901 gcttgatcaa cctgaccaac cgcgatccgg tggcggagat cgacctcggc cccgaaccgt
      961 actgggtgac gttcctcggt gagggctagc gcttggacga ccggctgcat cgggcgcgct
     1021 cgtccaggcc gtcaaccgcg tggcgcgtcg gggcggtcgc gttgacggtc gtggcgacgg
     1081 cttgggcggt cacgtcgcgc tcgtcggcga gggatctcag cggattcgtc gagtacgcct
     1141 acggcgaggg taccgaccgg cggacggacc cgcccgcacc ggaggtcgag cgggagtcca
     1201 gcttcttcgg gcagcgctac aacgtcgtct ggaatcgtca gatctacgag aatctccggt
     1261 tgctcaccgg cgggtacttc gaaggtctgg accaacagca gcagctcggt ggtctcgtca
     1321 ccgactcttc gatcacgacg ttcaggccgt tcgtcaacct gctgctcagc tcgcccgttt
     1381 ggtcgagcga gctcggctgg gatcgcaacg agcaatcgac cgacccgggc gtgggagccg
     1441 gcctgacgtt cgtccgcgac aactaccggg cgacgttcgg ctgggagccg gatcgatacc
     1501 cgctcgtcca acttcgcctg ctccgcaccg acgaccgcga tcggcagcgc tcgctgcgcg
     1561 atcgtgactt tcgcacggtg cagctgatct cggaatacaa cccgcgcgac ccggtctatc
     1621 tgtactacac cggcgcctgg cgccgcacgg aagacgcgat caacgacaat accgtgaccg
     1681 acgtctcgca caccgggcgc ttcgtctaca acgactccta cctgaatcgc cgcgcgttga
     1741 tcaactcgga ttggaccggt acctatcgca cgaccgacac gacgacctcc ggaacggggg
     1801 agatcagtct gcccgtcttc ccgttcgagg gtctgtcgga gatcgacgag tttccggaac
     1861 agggaccgct cgcgcggaac gcggcgctga tcgacgacga cttcgacacc agcgccggga
     1921 tcgacctcgg gctaccccca ccggcggggg acgaccgctc gcgcaacttc ggactggact
     1981 tcgccgacac cgtcgagatc aacacgctgt tcgtctggat cgaccgcgac atcccgctcg
     2041 acaccgcggc gtcgttttca tggcggatct acaccagcga cgacaaccag gaatggacgc
     2101 tgcgcgacag cgtgcccgcc gccgtcttcg gtggcgcatt cctgccacgg ttcgagatcc
     2161 gcttcgacaa cgtcgaggcc cgatacgtca agctggtgac cgagccgctg cagtcgaccg
     2221 accccgacgc gggtgatttc ccgtcgatct tcgtcaccga gctcgaagcg ttcatccgct
     2281 cgccggctgc cgacgtcgag ggcacgcgct cgctgttttc ccagatctac aacctcaatt
     2341 tccgctaccg catcaccgac gcgccgcgct tgttctacga gctgggttac tttttgaccg
     2401 acacggacgc cggaccgtct agctgggtgc tgtcgaacgg attctccgtg ttccagcgct
     2461 tcgcccgcat ctacacgctc aacgcgcgct acgcgcggga ggagatccgc gactcgagcg
     2521 tcgaacgcga cgcgaatcag ttgaccggct cgttgagcgc cgacccactg gagaccgtgc
     2581 gcttcagcct ggtctacagc gggcgggagg aggattcgtt cttcggcacg accgagggcc
     2641 agtcgctgtt cctgctgacg gtgctcgagt tgtaccgcgg ggtcagcctg aacgccggtt
     2701 tcggccggtc ctaccgcgag atcgaccccg gccggttgac cgagtcgcgc caggccaacg
     2761 ggacgctgac gatgcaaccc catcggacac tggcgatgac gctgttctgg caggaccgcc
     2821 gcgacgatct cagcggcggc gggctgaccg acgagcggcg cgtgacccgc agcaccgagt
     2881 tgaacgcggc ctggcgaccg gtgcaggcgc tatacttgtt cggcgcgttc cgcgtcgagg
     2941 atcgacccga cgtcgacgct cggaccacga gccgttacct gttcagttgg gctccgtttc
     3001 gcgacggaac gctgagcctg gtgctcaact acaacgaaac gctcaggtcc gatttcgata
     3061 cgagggagac ggtttcgagc tcgcgcattc gctggcaggt cagcggcaat acgttcgtca
     3121 acctggggtt cgccaagatc acgcaggaat ccgatctgtt cgactccgac tcgtcgaacc
     3181 tgagcgccga tgtgcgtttc ggtttttgac tcgaggttgt cgcgagggga atcatgagcc
     3241 gaatcgctcg aaccatcagg ctggcgcccg tcgtcgtgct gctgctcggc gccgcctgcg
     3301 cccacaagac gcagaaggtg acgttccacg accccaacat ggatttcggc ctcctgcgtt
     3361 ccgtcgcggt gatgccgttc ggcaacgaga cggcggaccg cagcgccgcc gaccgcgtgc
     3421 gggacgtgtt catgacgacg ctgcaggcga ccggtgcgtt ctacgtgctg ccgccgggtg
     3481 aagtcgcccg cggaatctcg cgcgtttcac cggaggacga gacggcgccg actcccgaca
     3541 acgtcgtcgc gttggccaac acgctcgagg tcgacgccgt catcaccggc gtcgtgctcg
     3601 agtacggcga ggtgcgctcc ggaacggcgt cggccaacgt gatcacggtc agcctgcgca
     3661 tgctcgaggc gcagagcggt cgggtggtct ggagcgcgtc gacgaccaag gggggtgtca
     3721 gcgcctccga tcgtctgttc ggtggcgggg ggcagccgat gaacctggtg acccagcgcg
     3781 cgatcgacga gctgctggac agcctgttca gcggctgatc gcggggtgga gccgatgatc
     3841 cgcgatcgat cttcacgcgt ggcccgggcg ttgtgcacag ttgccgtgtc cctcgtcgcc
     3901 gcgcgggcga tcgccgcgca aacgcccgtg cccgtcggac gcgaggcacg gatcgcggtg
     3961 ctgccgatgg tcaacctgtc ggccaaacgc gcaccgatgg acgagctgcg cgcgctgctc
     4021 ggcgaccacg ccgacttgcg cggactcgag acgttgcggc ctgcggagct cgagaccttc
     4081 atgtcctcgc gccggatgcg ctacacgggc ggcgtcgatt cggcgaccgc ggtggcgctg
     4141 cgggagtcga ccggtgccca agcggtgctg atcagcgcgt tgcactcata cgacgaggcg
     4201 gacccgccga cgttcgcgat cgtggctcgg ctggtcacga ccgagagcga cccgcggatc
     4261 gtctggatgg cggaggtcag tctgaccggc gaccagacgc cgggactgct cgcgctgggc
     4321 ctggtacgcg acgtcgccac gctgcgcgat cgcgccttcg agcagttgtt cgactcgctg
     4381 gtcgagcagc tcggctcggc cgagccgccg cgtgtcgggc gcctgaaggc caagaaccgg
     4441 ttccgcccga agcggttcta ccgtacgccg caacggcccg agagcgacga cgcggcggta
     4501 cgcgtcgcgg tcctgccgtt catca
//
LOCUS       DASPGN010000262         9842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1567031, whole genome shotgun sequence.
ACCESSION   DASPGN010000262 DASPGN010000000
VERSION     DASPGN010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567031"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VD788_07700"
     CDS             complement(<1..437)
                     /locus_tag="VD788_07700"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HXV76189.1"
                     /translation="MATFDISARLRSRLRRDRPVVALTGAGISAESGLATFRGPGGLW
                     EGRDPLELATPEAFARDPQTVWRFYGWRRRQALAARPNAAHLALARWRRHAPGFTLIT
                     QNVDGLHERAGNHDLIRLHGTLWRLRCVTGDREFDDQRENVGEP"
     gene            545..1156
                     /locus_tag="VD788_07705"
     CDS             545..1156
                     /locus_tag="VD788_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV76190.1"
                     /translation="MSEPTPQERRDEFEEVALPHLDALFNLALNLTRNRKDAEDLVQE
                     TYLRAYRFFDSFQPGTQIKAWLFRILRNTFINRYRAAKARPDEVDFARIDGGFESQVD
                     DDYLRSHGPPDPEQALMTGVPDETIRQAMAELPEEYRTVVLMALLEEMSYKEIAAALA
                     IPLGTVMSRLHRGRRHLRRALHDYARRRGIIRDRPGVFRDGIA"
     gene            1185..1847
                     /locus_tag="VD788_07710"
     CDS             1185..1847
                     /locus_tag="VD788_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV76191.1"
                     /translation="MNCQHFRDRLFLFQDDELPEPERDECQGHLDGCPACAEILAGEE
                     IGLRVLKSKLERTPAPPGLDTRIRLALRAEGAGGRRRVWFRTPSFAAVAAATLLLALV
                     VPTLIDERSPVPAGLHVHELVTVVDLDCDRAGRSLALQRRCSHPLHVNALKRADGTYW
                     AVSPQQSEFRYLWLGREARGRSLVVEGRLFPETETIRLDAVERLEACLIPLPPVFGWR
                     SC"
     gene            1932..2255
                     /gene="trxA"
                     /locus_tag="VD788_07715"
     CDS             1932..2255
                     /gene="trxA"
                     /locus_tag="VD788_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXV76192.1"
                     /translation="MSQVLEANDSNFDAEVLKASGPVLVDFSATWCGPCKKLDPIVRE
                     IAGDYAGRLKVVKVDVDRAPGTAAKFGVMSVPTVLLVRDGAVRDQLIGLVSKQALADR
                     VDKVL"
     gene            2257..2997
                     /locus_tag="VD788_07720"
     CDS             2257..2997
                     /locus_tag="VD788_07720"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HXV76193.1"
                     /translation="MQELLTTWGQQLQQILNDQLQSGSLSAVFVVFAAGVLTSFTPCV
                     YPMIPVTVTYIGGAAAGNRLRACSLSAVYVGGLALIYATLGVVTALLGRQFGSFTREP
                     WIYAGVAVLITLFGVTMLGWIEIPVPGFFGKIQSTGVRRGGHLGALLIGMAAGFVAAP
                     CTAPVLGLLLVYVANTRDVVWGGSLLLIFALGLGLLLMILGISSGLLASLPRAGRWMD
                     RIKTVFGAGMLVIAAWFFYQAAMIYIQS"
     gene            3008..3526
                     /locus_tag="VD788_07725"
     CDS             3008..3526
                     /locus_tag="VD788_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXV76194.1"
                     /translation="MRGRTLQFRAWGFALALALVPAAVAAGAGDPFGQTLVRTADGRS
                     VELAPGAPALHVVFLATWCPPCLDEFEALAELEARWGERGYRLVLVPVKTRHTIERLR
                     EFDEERRLPGELLFDSQGAAQTAAGATELPTHLLYDASGKQVLRSGAFADGVAAAVAS
                     LLENSTPRSPRR"
     gene            3523..4641
                     /locus_tag="VD788_07730"
     CDS             3523..4641
                     /locus_tag="VD788_07730"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HXV76195.1"
                     /translation="MKPTRVGLLFGGRSVEHEVSLSSARGVARAMGETALVCVPIGVT
                     PEGRWLSPEVSRAILDGDGARVEPPAGGDDGDRIVLDPGRRGMQRVTADRTARPLAVD
                     VVFPLVHGWGGEDGRLQGALDLAGLPCVGAGVLGSAVGMDKAVAKSLLERHGVPVTPW
                     ILATRARFARAPAAVVDQVLAALRPPLFVKPANGGSSVGITKVERPGGLADALEFAFA
                     HDRRVLVERGIDAREIECAVLGNDEPEASGLGEIVPSREFYDYEAKYLDDASRLIIPA
                     ELDGAQGERLRRQAVEAYRALDLAGFARVDFLVERNGDRYFLNEVNTLPGFTPISMFP
                     KLWDAAGLPFPRLIERLVSLALERATDESTRRTARTTG"
     gene            4666..5286
                     /locus_tag="VD788_07735"
     CDS             4666..5286
                     /locus_tag="VD788_07735"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXV76196.1"
                     /translation="MAETAGSVLVLNSLYQAVQVTGVRRAFRLFYAGRARAVAPDFAS
                     YDFDNWCDLPPRAQDEVIHTPTRAIRIPKVIQLIQYDRLPSREIRFTRRNIFYRDKNR
                     CQYCGQVFRQKDLNLDHVVPLSRGGKSNWGNVVCACIPCNTRKGDRTPVEAHMRLVRR
                     PKKPAGHPMLRANWIGPCPDEWKTFLDEAYWNVELHDDVMLHRADG"
     gene            complement(5291..6703)
                     /locus_tag="VD788_07740"
     CDS             complement(5291..6703)
                     /locus_tag="VD788_07740"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV76197.1"
                     /translation="MTPSNQRPGSPEATRENVEHVLQLALDTTGAERAFFVRGRPAGD
                     GSLDPVVFAWRTRADDGVPHPSRSLARAALTRREAFLSLDAQAGDAWSEAASVRAMAL
                     RFVLVAPLPVRCGCRSALVLDSRSAPRASPRPELIRSAVLCARLIDQLTEAATPSLVS
                     AGEADGPRLVGRSRPIRETWTWIERAARTQLPVLILGETGSGKEGVARRIHDGGPRRA
                     APFVAFNCAALPDSLIEAELFGSVRGAYTGAERDRRGLFALADGGTLLLDELGDMPAS
                     MQAKLLRALEEGRVRPIGADRDVAVDVRIVGATHHDLAQRVRDGAFRADLYHRLAVIE
                     IRVPPLRERIDDLPLLIESLAPRLERETGRALPPLGRASLDILGRHSWPGNVRELHAV
                     LARALLRADGAPIDPRHLAELCRQARTLDNANDGSERAMIAAALANTDGSVGAAARRI
                     GWTRQKLYRRINALALDRLL"
     gene            6811..7584
                     /locus_tag="VD788_07745"
     CDS             6811..7584
                     /locus_tag="VD788_07745"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXV76198.1"
                     /translation="MSYYRQGPPPGSGFPLGVPQVTPMMRSLMIATGAVWLLQILLGS
                     FGFRLFGLGFEEIFGVVPLLFVRGMLWQPLTYVFLHSPSSLMHILFNMLFVWMFGGDL
                     ERLWGGREFLRYYLITGAGGGVLAALMGLAGGWLGSPADYYSVTIGASGAVFGLITAF
                     GMIFGERTVLFMLLFPMKARTMAMILFAIAFFYTFTGGGGNVSHVGHLGGALVGFLYL
                     KRAWRVGAFYREIKWRIRRRRFKVLTRDDRKDDFDRWVH"
     gene            8094..8990
                     /locus_tag="VD788_07750"
     CDS             8094..8990
                     /locus_tag="VD788_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HXV76199.1"
                     /translation="MDSTHNERSSVLAKYFSEIRSYPLLTKKQEQDLAGRVRDGSREA
                     LHELIESNLSFVVKVATEYRNLGLPFEDLLNEGNIGLIEAAHRYDASKGTKFITYAIW
                     WIRKSILKALSEHSSLVRVPTYQMKKVREIRDAENSLRRSLGRKPQREEISERLARSL
                     AKIDQVLQFSMREMSLDEKVGKDRETPISDYLVDDASASPEDDLIQREANSLVSEAMH
                     QLSEQEKVVIAYRFGIAGGPTLTLKEIGEIMSISRERVRQIECQAKSRLRKIFARKRV
                     VKAPPKRPFPVGGVSKRVRLDS"
     gene            9031..>9842
                     /locus_tag="VD788_07755"
     CDS             9031..>9842
                     /locus_tag="VD788_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76200.1"
                     /translation="MVEHPETGLDDPDEPRQPDASAAIDAMLAARTRELHLIQLLGRR
                     AAEAKSVDDLFRAAISIVQEAAGLDLALVAFEHGGRRDLTCHLSRPCAGPAVERLSRL
                     AGTVLGWPADDRLPTAVEVLAEEFDEGRGVRTGFAADDPIVVPVMRRARPVAIMVFLP
                     AGSPSEAGIRLLFSATNQLAVHLDRILTAREEEVDRFRSILDAMLQPVVLTDAGLGIV
                     HLNASGRALLDRLGLEGAHGFDAVVERLGIAAEVGRVLERGEAWTDGEARLA"
BASE COUNT         1487 a         3335 c         3425 g         1595 t
ORIGIN      
        1 ggttcgccga cgttttcgcg ctggtcgtcg aactcgcgat cgccggtgac acagcgcaac
       61 cgccacagcg tcccgtgcaa ccggatcagg tcgtgattgc ccgcccgctc gtgcaggccg
      121 tcgacgttct gcgtgatcaa cgtgaagccg ggcgcgtgac gtcgccagcg ggcgagcgcg
      181 agatgggccg cgttcggtcg agcggcgagc gcctggcgtc ggcgccaacc gtagaagcgc
      241 cagacggtct gcgggtcgcg cgcgaacgcc tcgggcgtgg ccagctccag cgggtcccgc
      301 ccctcccaca acccgccggg gccacgaaag gtggccaaac cgctctcggc ggagatgccg
      361 gcgccggtca gcgccaccac cggccggtct cgtcgcagcc gcgaacgcag gcgggctgag
      421 atgtcgaacg tggccatcgg tcggttctcc gctccggccg tgcatcgtac cgcgaattga
      481 ccggtcgctg cttccacgta tagttggacc acccgcgccg cccggcgagc gggtcggagc
      541 cccggtgagc gagccaacgc cgcaagagcg cagggacgag ttcgaggagg tcgcgctccc
      601 gcacctcgac gccctgttca acctggcgct caacctgacg cgcaaccgca aagacgccga
      661 ggatctggta caggagacct acctgcgggc ctaccgcttc ttcgactcgt tccagccggg
      721 gacgcagatc aaggcctggc tgttccggat cctgcgcaac accttcatca atcggtaccg
      781 cgcggcgaag gcccggcccg acgaggtcga tttcgccagg atcgacggcg gcttcgagtc
      841 gcaggtcgac gacgactact tgcgcagcca cggcccaccc gacccggagc aggcgctgat
      901 gaccggcgtg cccgacgaga cgatccggca agcgatggcc gaacttccgg aggagtaccg
      961 cacggtggtg ctgatggctc tgctggaaga gatgtcgtac aaggagatcg cggccgcgct
     1021 ggcgatcccg ctggggacgg tgatgagccg cctgcaccgc ggccggcgcc acctgcggcg
     1081 ggcgctccat gattacgccc gccgccgggg aataatccgc gaccgaccgg gggttttcag
     1141 agacggcatc gcgtgacgag cgccgcgtcg gagcgggaga gccgatgaac tgccagcatt
     1201 ttcgcgaccg gctgttcctg ttccaggacg acgagctgcc cgagcccgag cgcgacgaat
     1261 gtcagggcca tctcgacgga tgcccggcgt gcgccgagat cctcgccggc gaggagatcg
     1321 gcctgcgcgt gttgaagtcg aagctcgagc gcacgcctgc gccgcccggg ctcgatacgc
     1381 gtatccggct cgcgctgcgg gccgagggag cgggtggcag gcgccgggtg tggttccgca
     1441 cgccgtcgtt cgccgccgtc gccgcggcga cgttgctgct cgcgctcgtc gtgccgacgt
     1501 tgatcgatga gcgttccccg gtgccggcgg gcctgcatgt ccacgagctg gtgacggtgg
     1561 tcgatctcga ttgcgatcgt gcgggtagga gcctcgcgct gcagcggcgc tgctcgcacc
     1621 cgctgcacgt gaacgcgctc aagcgggccg acggaaccta ctgggcggtc agtccccagc
     1681 agtccgagtt ccgctacctg tggctcggcc gcgaggcccg cggccgatcg ctcgtcgtcg
     1741 aaggccgact gttccccgag accgagacga tccgactgga cgcggtcgag cggttggagg
     1801 cctgtctgat cccgttgccg ccggtgttcg gctggcgttc gtgctaggct gaccgacggg
     1861 cgcgcgtagc caccggcccg atccgacgag gcgttccacc cacacccaac atcgatcttg
     1921 cgagggagtc catgagccag gttctcgaag ccaacgacag caacttcgac gccgaagtgc
     1981 tgaaagcgag cgggccggtc ctcgtcgatt tttccgcgac gtggtgcggg ccgtgcaaga
     2041 agctcgatcc gatcgtgcgc gagatcgccg gtgattacgc agggcgcctc aaggtcgtca
     2101 aggtcgacgt cgaccgggca cccggcaccg ccgccaagtt cggcgtgatg tcggttccga
     2161 ccgtgctgct cgttcgggac ggcgcggtcc gagaccagtt gatcgggctg gtctccaagc
     2221 aggcgctggc cgaccgggtc gacaaggtcc tttgacatgc aagagttgct gaccacctgg
     2281 ggccagcaac tgcagcagat cctgaacgac cagctgcagt cgggatcgct ttccgcggtc
     2341 ttcgtcgtgt tcgcggcggg cgtgctgacg agcttcacgc cgtgtgtcta tccgatgatc
     2401 ccggtgacgg tgacctacat cggcggtgcg gccgcgggca accggctgcg ggcctgctcg
     2461 ttgtcggcgg tctacgtcgg cgggctcgcg ctgatctacg cgacgctcgg cgtggtgacc
     2521 gcgctgctcg gcaggcagtt cggcagcttc acccgcgagc cgtggatcta cgccggggtc
     2581 gccgtgctga tcacgctgtt cggcgtgacg atgctcggtt ggatcgagat cccggtgccg
     2641 ggcttcttcg gcaagatcca gtcgacgggc gtgcgacgtg gcggtcatct cggcgcgctc
     2701 ttgatcggca tggcggcggg cttcgtcgca gcgccgtgca ccgcgcccgt gctcgggttg
     2761 ctgctggtct acgtcgccaa cacgcgggac gtggtgtggg gtggctcgtt gctgctgatc
     2821 ttcgccctcg gcctcggcct gctgttgatg atcctcggca tatcctccgg cctgctggcc
     2881 agcctgccgc gcgccgggcg ctggatggac cggatcaaga cggtcttcgg cgccggaatg
     2941 ctggtcattg ccgcctggtt tttctatcag gccgcgatga tctacattca atcctgaggg
     3001 tcgatcgatg aggggacgaa ctctgcagtt ccgggcgtgg ggcttcgcgc tggcgctggc
     3061 cctggtgccg gccgccgtcg ccgccggagc cggcgacccg ttcggccaga cgctcgtgcg
     3121 caccgccgac ggacggtcgg tcgagctcgc gccgggcgcc cccgcgcttc acgtcgtgtt
     3181 cctcgccacc tggtgcccgc cgtgtctcga cgagttcgag gcgctggccg agctcgaggc
     3241 gcgctggggc gagcgtggct atcggttggt cctggtgccg gtcaagaccc gccacacgat
     3301 cgagcggctg cgcgagttcg acgaggaacg gcgactcccc ggcgagctgc tgttcgactc
     3361 gcagggcgcc gcgcagacgg cggcgggcgc gaccgagctg ccgacccatc tgctttacga
     3421 cgcctcgggc aagcaggtgc tgcgatccgg cgcgttcgcg gacggggttg ccgcagcggt
     3481 cgcttcgctg ctcgagaatt ccacgccgcg gagcccgcga agatgaaacc cacccgcgtc
     3541 gggctgttgt tcggtggtcg atcggtcgag cacgaagtct cactcagctc cgcccgcggc
     3601 gtggcgcgcg cgatgggtga gaccgcgctg gtctgcgtgc cgatcggcgt gacgcccgag
     3661 ggccgttggt tgtcgcccga ggtctcgcgc gcgatcctcg acggcgacgg cgcgcgggtc
     3721 gagccgcccg cggggggcga cgacggcgac cggatcgtgc tcgacccggg acggcgggga
     3781 atgcagcgcg tgacggccga ccggacggca cgcccgctcg cggtcgacgt cgtcttcccg
     3841 ctggtccacg gctggggcgg cgaggacggt cgcctgcagg gcgcgctcga cctggccggt
     3901 ctgccctgcg tcggcgccgg cgtgctcggt tcggcggtcg gcatggacaa ggcggtcgcc
     3961 aagagtctgt tggagcggca cggggtcccg gtcacgccgt ggattctggc gacgcgggcg
     4021 cggttcgctc gggcgccggc cgcggtcgtc gaccaggtgc tcgccgcgct gcgcccgccg
     4081 ctgttcgtca aaccggcgaa cggcggctcg tcggtcggca ttaccaaggt cgagcggccc
     4141 ggcgggttgg ccgacgcgct cgagttcgcc ttcgcccacg accggcgggt gctcgtcgag
     4201 cgggggatcg acgcccgcga gatcgagtgc gcggtgctgg ggaacgacga gccggaagca
     4261 agcggcctgg gcgagatcgt accctcgcgc gagttctacg actacgaggc caagtacctc
     4321 gacgacgcct cccgtctgat catccccgcc gagctcgacg gtgcgcaggg agaacggttg
     4381 cgccgccagg ccgtcgaagc ctaccgcgcg ctcgacctgg ccgggttcgc ccgcgtcgat
     4441 ttcctggtcg agcgtaacgg ggaccggtat tttctcaacg aggtgaacac gctgcccgga
     4501 ttcacgccga tcagcatgtt cccgaagctg tgggacgcgg ccggcctgcc gtttccgcga
     4561 ttgatcgagc ggctggtctc gttggcgctc gagcgcgcga cggacgagtc gacgcgcagg
     4621 accgcacgaa cgacgggctg aggcggtccg gggttggtga actcgatggc ggagacagcc
     4681 ggttccgtgc tcgtgctcaa cagcctgtac caggcggtgc aggtcactgg cgtgcggcgc
     4741 gcgttccgcc tgttctacgc cggtcgcgcg cgggccgtcg cgccggactt cgccagctac
     4801 gacttcgaca actggtgcga cctaccaccc cgggcgcagg acgaggtgat ccatacgccg
     4861 acgcgggcga tccggatccc caaggtgatc cagttgatcc agtacgaccg gctgccgagc
     4921 cgcgagatcc gcttcacgcg ccgcaacatt ttctaccgcg acaagaaccg ctgccagtac
     4981 tgcggccagg tcttccgcca gaaagatctc aacctggacc atgtcgtccc gctgtcgcgc
     5041 ggcggaaagt cgaactgggg caacgtcgtc tgcgcctgca ttccgtgcaa cacgcgcaag
     5101 ggcgaccgca cgcccgtcga ggcgcacatg cggctggtcc gccgcccgaa gaaaccggcc
     5161 ggccacccga tgttgcgcgc caactggatc gggccgtgtc ccgacgagtg gaagaccttc
     5221 ctcgacgagg cctactggaa cgtcgagctg cacgacgacg tgatgctcca ccgcgcggac
     5281 ggctgacgcg tcacaacaag cggtcgagcg ccagcgcatt gatcctgcgg tagagcttct
     5341 gacgcgtcca gccgatccgg cgtgccgccg ccccgaccga gccgtccgtg ttggccagcg
     5401 cggccgcgat catcgctcgc tcggagccgt cgttggcgtt gtccagcgtt cgcgcctggc
     5461 ggcacagctc ggcgagatga cgcggatcga tcggcgcgcc gtcggcgcgc agcagcgctc
     5521 gagccagcac cgcgtgcagc tcgcgcacgt tgcccggcca actgtgccgg ccgagtatgt
     5581 cgagcgaggc tcgcccgagc gggggcagcg cccggccggt ctcccgttcg aggcgcgggg
     5641 cgaggctctc gatcagcaac ggcagatcgt cgatccgctc gcgcagcggc gggacgcgga
     5701 tctcgatcac ggccagacgg tggtacagat cggcgcgaaa cgcgccgtcg cgcacgcgct
     5761 gcgccaggtc gtggtgggtc gccccgacga tacgcacgtc gacggcgacg tcgcgatcgg
     5821 cgccgatcgg ccggactcgg ccttcttcca gcgcgcgaag cagcttggcc tgcatcgacg
     5881 cgggcatgtc gcccaactcg tcgagcagca gcgtgccgcc gtcggccagt gcgaacagtc
     5941 cgcgccggtc gcgctccgcc ccggtgtagg cgccgcgtac gctgccgaac agctccgcct
     6001 cgatcagcga atcgggcagc gccgcacagt tgaacgcgac gaacggcgcc gcacggcgcg
     6061 gaccgccgtc gtggatccgc cgggcgacgc cttccttgcc cgatccggtc tcgccgagga
     6121 tcaacacggg cagctgcgtg cgcgccgcgc gctcgatcca cgtccacgtc tcgcggatcg
     6181 gccgcgagcg accgacgaga cgcgggccgt cggcctcgcc ggcagagacg agcgatggcg
     6241 tcgccgcctc ggtcagctgg tcgatcaagc gcgcacacag gaccgcgctc ctgatcaatt
     6301 cgggtcgtgg gctcgcgcgc ggcgccgatc ggctgtcgag caccagcgcc gatcggcatc
     6361 cgcaccggac cggcagcgga gccaccagca cgaagcgcaa cgccatcgcg cgcaccgacg
     6421 ccgcctcgct ccaggcgtcg cccgcctggg catcgagcga caggaaggcc tcacggcgcg
     6481 tcagcgccgc gcgcgccagc gagcggctcg gatgcggcac cccgtcgtcg gcgcgcgtac
     6541 gccaggcgaa gaccaccggg tcgagcgagc cgtcgcccgc cggccgccca cggacgaaga
     6601 acgcccgctc ggcccccgtc gtgtcgagcg ccagctgcaa cacgtgctcg acgttctcgc
     6661 gtgtcgcttc cggcgaaccc ggtcgttggt ttgacggcgt cacggtgtgc gggatatgct
     6721 cggctttcgt gctcgaccgc cgcaagaacc gccgcatccg gggcggtttc gagagttgaa
     6781 ctcccaagcg tacgacggcc gcgaggttcg atgagctact accgtcaggg tccccctccc
     6841 gggtccgggt tcccgctggg cgtcccgcag gtgacgccga tgatgcggag cctgatgatc
     6901 gcgacggggg cggtgtggct gctccagatc ctgctcggat cgttcggctt ccgcctgttc
     6961 gggctcggtt tcgaggagat tttcggcgtc gttccgttgc tgttcgtccg cggaatgctc
     7021 tggcagccgc tgacctacgt cttcctgcac agcccgagca gtctgatgca catcctgttc
     7081 aacatgctgt tcgtctggat gttcggcgga gacctcgagc gcctgtgggg tgggcgggag
     7141 ttcctgcgct actacctgat caccggggcc ggcggcgggg ttctcgccgc cctgatgggg
     7201 ctcgccggcg gctggctcgg ttcaccggcc gactactaca gtgtcacgat cggcgcttcg
     7261 ggggccgtat tcggcctgat caccgcgttc ggcatgatct tcggcgagcg caccgtgctg
     7321 ttcatgctgc tgttcccgat gaaggcacgg acgatggcga tgatcctgtt cgctattgcg
     7381 ttcttctaca ccttcaccgg cggcgggggc aacgtctcac acgtcggcca tttgggcggc
     7441 gcgctggtcg gattcctcta cctgaagcgc gcgtggcgcg tcggtgcgtt ctatcgagag
     7501 atcaagtggc ggatccgccg ccgccgcttc aaggtgttga cccgggacga ccgcaaagac
     7561 gacttcgatc gctgggtcca ctgacagtta tctggcgtgc gctcgcgccc gccggatctg
     7621 ccgcgaatat cattgcgagt ggcggatctc cgtattcgtc ggtagggttt ggatggcgat
     7681 ttcgtcagcg gaaggtcggt cgagaacacg cgtcaggcta atcgcgaaaa cctctaaaac
     7741 tcaagccggg cgcgggtttt cgaaaacaat tcgatgttga cacgtttcga aaccgtgcat
     7801 agactaacga ctccggagca agattccaac cagtacggaa aggctcgggc tcggtcgcgc
     7861 gacgcgatca tttcgggggg ggagcgggtg tcccgatcgg tcggccgcac agtctcaggc
     7921 acgaaccgcc cgcatttcgc tttcgtctct acgcgcccgg acccgcactc ccgttggatg
     7981 aggcgagaaa ctacgccgat cgattccgac ggtcaagaaa actctcaccg agcgcgcggt
     8041 tcatttgcag cgcccgcaag cggctcccgg cgatcgaagc tcgaggcacc ttgatggact
     8101 ccacacacaa cgaacgaagc tcggtcctgg cgaagtactt ttccgaaatc cgcagctacc
     8161 cgctcttgac caagaagcag gagcaggact tggccggccg cgtccgcgac ggctcgcgcg
     8221 aggcgctgca cgagttgatc gagtcgaatc tcagtttcgt ggtcaaggtc gcgaccgagt
     8281 accggaatct cggtctgccg ttcgaggatc tgttgaacga gggcaacatc ggcttgatcg
     8341 aagccgcgca ccgatacgac gcctcgaagg gcaccaagtt catcacctac gcgatctggt
     8401 ggatccgcaa gtcgatcctc aaggcgctca gcgagcactc gagcctggtc cgcgtgccga
     8461 cctaccagat gaagaaggtc cgcgagatcc gcgacgcgga aaactcgttg cgacgcagcc
     8521 tcggtcgcaa gccgcagcgg gaagagatct ccgagcgact ggcacgcagc ctggccaaga
     8581 tcgaccaggt gctccagttc tcgatgcgcg agatgagtct cgacgagaag gtgggcaagg
     8641 accgcgagac gccgatctcc gactacctcg tcgacgacgc ctcggccagt cccgaggacg
     8701 atctgatcca gcgcgaggcc aactcgctgg tcagcgaggc gatgcaccag ctgagcgagc
     8761 aagagaaagt cgtcatcgcc taccgtttcg gcatcgccgg cggaccgacg ctgacgctga
     8821 aggaaatcgg cgagatcatg agcatcagtc gcgaacgtgt gcggcagatc gagtgccagg
     8881 ccaagagccg cctacgcaag atcttcgcac gcaagcgggt ggtcaaagcc ccgccgaagc
     8941 ggccgtttcc cgtcggcggc gtgtcgaagc gggtccgtct cgattcctga cccttgatcg
     9001 cccaccgggt tgtccctaga ctcgcacgcg gtggtcgaac atcccgaaac cggtctcgac
     9061 gatccggacg agccccggca gccggacgcg agcgcagcca tcgatgcgat gctcgcggcc
     9121 cgcacccgtg agctccacct gatccagctc ctcggccgac gcgccgccga ggccaagtcg
     9181 gtcgacgatc tgttccgcgc cgcgatctcg atcgtgcagg aggccgcggg gctcgacctc
     9241 gctctggtcg cgttcgagca cggcgggcgg cgcgacctga cgtgccacct gtcccgtccg
     9301 tgcgccggcc cggcggtcga gcggctgtca cgtctggccg gcaccgtgct cggctggccg
     9361 gcggacgatc ggctgccgac ggcggtcgag gtcctcgccg aggagttcga cgaagggcgc
     9421 ggcgtgcgca ccgggttcgc ggcggacgat ccgatcgtcg tgccggtgat gcggcgggcg
     9481 cgcccggtcg cgatcatggt cttcctgccg gcgggttcgc cgagcgaagc gggaatccgc
     9541 ctgctgttca gcgcgaccaa tcagctcgcg gtccacctcg atcgcatctt gacggcacgc
     9601 gaggaggaag tcgaccgatt ccgctcgatt ctcgacgcga tgctgcagcc cgtcgtgctg
     9661 accgacgccg ggctgggcat cgtccatctc aacgcctcgg ggcgcgcact gctcgatcgc
     9721 ctcggactgg aaggcgcgca cggattcgac gccgtcgtcg agcgactggg gatcgcggct
     9781 gaggtcgggc gcgtgctcga gcgcggcgaa gcatggaccg acggcgaggc gcgactcgcc
     9841 ga
//
LOCUS       DASPGN010000263         4335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_280928, whole genome shotgun sequence.
ACCESSION   DASPGN010000263 DASPGN010000000
VERSION     DASPGN010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280928"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2224)
                     /locus_tag="VD788_07760"
     CDS             complement(<1..2224)
                     /locus_tag="VD788_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76201.1"
                     /translation="MSIRSVNFSLISTLTLALLVAAPLGAAPPPQANRPKVEFDEQMT
                     PLQRAVDRAVEERKERERGNPDNNGRCNQAIEENDPDDIAMFCKVPHVGMKKLDPTEC
                     GDCGEIFNPAGQMVGKRIRTETTLDYSEPGQRGIPDENGEGRWQKVMVERPRPRGIGN
                     GVSDRGLGRAGGARVHNSVLFPEYAVNEDRDCIDTVTGERFGGYVGGQIPADGDPASC
                     FDASGELKRTLEVAAGGCRHAASGEMLDGDMADFLDDPCFDERNELKTSLIESIDEDG
                     PENLDNDNDGLFAEDPAGNGNEDNDCIDSSGIVQRDAACFDASGALLEGKSELIDEDG
                     DDLIDDDRDGRINEDAPAADALAGCRDFGSRAKGLPPGLGDVMPDGECDLTRAMIVEM
                     NRQAMMPVDQGGLGLSGKVFLADDQGNYDPSVAADLDHDGTTDSVEFGRERRRIKLSE
                     AFTLKCQDPAAEMVDGQCVSPPADDTAALAADPRASSSGLVQGRQQWTDNGDGTRTLK
                     QYMMMGFTFAPPVLEWGYRVEEYACIDLLFGEVCFEVFFARIGYEFDFAVGLRLPVEV
                     EVSDIPSDAVLAGQQLMLNTKITPVDFTAKQYEQFCEENDLADGLLIADCERFSFPNF
                     FDTLNPFTPEAEVDGDEFVAHELVFAGLIVRVIGIPIIGWGIDSAVDLPTMCSLVQTH
                     AQGFDLVQFGSDLAADKPVLEALKNQLINCGSFTTPFGLEQDPLGLPGVERLRAFPFS
                     A"
     gene            complement(2347..>4335)
                     /locus_tag="VD788_07765"
     CDS             complement(2347..>4335)
                     /locus_tag="VD788_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV76202.1"
                     /translation="LAVPVSLIGTFAGMMLLGFSINTLTLFGLVLAIGIVVDDAIVVV
                     ENVERHVRMGKSRIEAALVSARELVGPIIAMTITLAAVYTPIALQGGLTGALFREFAF
                     TLAGAVIVSGVVALTLSPMMGSRLLRAGDTERGFAGMINRHFEVVRRLYARVLDRTLE
                     WRPVVLTLWVLVALLSLPFYMLSMKELAPAEDQGVVFGIVQAAPNATLDQTRLFISQV
                     HQVYESIPEANHTFQITFPSGGFSGVVLKPWSERERTSQQAQLQVAAGLAKIPAVRVI
                     PLTPPALPGGGQYPIDLVVASTAEPEQLAEIADQLVVKAFQSGLFMFADSDLKFDQPQ
                     AEVVFNRDKLRSQGVDLSQAGSDLATMLSGDYVNRFSIQGRSYKVIPQIKRAERLTPD
                     QLEQIHVSGPDGRLVPLSTFATLRTTTVPRELKRFQQLNAVRIQGVIPPNVSLDQALS
                     YLEREAQAMLPSGFSLDHAGQSRQLRTEGRKFLATFLLSAVLIYLVLAAQFESFRDPF
                     IILAGSVPLAIAGSLMFSFLGLTTLNIYSQVGLITLVGLIAKNGILIVEFANQLQARG
                     LDKVNAVREAAATRLRPILMTTAATVVGHTPLVLASGPGAGARNSIGIMLVSGMVIGS
                     IFTLFVVPSIYVLVARRHPTAERPADAEADDRRLARAV"
BASE COUNT          679 a         1489 c         1472 g          695 t
ORIGIN      
        1 aggcgctgaa cgggaaggcg cgcagtcgct cgacgccggg cagcccgagc gggtcctgct
       61 cgagaccgaa cggcgtggtg aagctgccgc agttgatcag ctggttcttc agcgcctcga
      121 gcaccggctt gtcggcggcg aggtccgagc cgaactgaac cagatcgaaa ccctgcgcgt
      181 gggtctgcac cagcgagcac atcgtcggca ggtcgaccgc cgaatcgatc ccccagccga
      241 tgatcgggat gccgatcacg cggacgatca gtccggcgaa aaccagttcg tgcgcgacga
      301 actcgtcgcc gtcgacctcc gcctccgggg tgaacgggtt gagcgtgtcg aagaagttgg
      361 ggaacgagaa gcgctcgcaa tcggcgatca gcagcccgtc ggccaagtcg ttttcctcgc
      421 agaactgctc gtactgcttg gcggtgaaat cgaccggcgt gatcttggtg ttgagcatca
      481 gctgctggcc ggcgagcacc gcgtcggacg ggatgtcgct gacctcgacc tcgaccggca
      541 gtcgcaggcc gaccgcgaag tcgaactcgt agccgatccg cgcgaagaac acctcgaagc
      601 agacctcgcc gaacagcagg tcgatgcagg catactcctc gacgcggtag ccccactcga
      661 gcaccggcgg ggcgaaggtg aaccccatca tcatgtactg cttgagtgtg cgcgtgccgt
      721 cgccgttgtc ggtccactgc tgccggccct gcaccagccc cgagctgctg gcgcgcgggt
      781 cggcggcgag cgccgccgtg tcgtcggccg ggggcgagac gcactggccg tcgaccatct
      841 ccgctgctgg atcctggcac ttcagcgtga acgcctcgga cagcttgatc ctgcgccgct
      901 cgcggccgaa ctcgacgctg tcggtcgtgc cgtcgtggtc gaggtcggcg gcgacgctcg
      961 ggtcgtagtt gccctggtcg tcggcgagga acactttgcc ggaaagcccc agcccgccct
     1021 ggtcgaccgg catcatcgcc tggcggttca tctcgacgat catcgcgcgg gtcaggtcgc
     1081 actcgccgtc gggcatcacg tcgcccaacc cgggcggcag gcccttggcc cggctgccga
     1141 agtcgcggca gccggccagc gcgtcggcgg ccggtgcgtc ctcgttgatc cggccgtcgc
     1201 ggtcgtcgtc gatcaggtcg tcgccgtcct cgtcgatcag ctcgctcttg ccctcgagca
     1261 gggcgccgct cgcgtcgaag caggcggcgt cgcgctggac gatcccgctc gaatcgatgc
     1321 agtcgttgtc ctcgttgccg ttgccagccg ggtcttcggc gaacaggccg tcgttgtcgt
     1381 tgtcgaggtt ctcgggaccg tcctcgtcga tcgactcgat gagagacgtc ttgagctcgt
     1441 ttcgctcgtc gaaacagggg tcgtcgagaa agtcggccat gtcgccgtcg agcatctcgc
     1501 ccgaggcggc gtgacggcag ccgccggcgg cgacctcgag cgtgcgcttc agctcgccgc
     1561 tcgcgtcgaa gcacgaagcc gggtcgccgt ccgccgggat ctggccaccg acgtagccgc
     1621 cgaatcgctc gccggtcacc gtgtcgatgc aatcgcggtc ctcgttgacc gcgtactcgg
     1681 ggaacagcac gctgttgtgc acccgcgcgc cgcccgcacg accgagcccg cgatcggaga
     1741 caccgttgcc gatcccgcgc ggccgcggtc gctcgaccat caccttctgc cagcggcctt
     1801 cgccgttctc gtcggggatg ccgcgctggc ccggctcgct gtagtcgagc gtcgtctcgg
     1861 tgcggatgcg cttgccgacc atctggcccg ccgggttgaa gatctcgccg cagtcgccgc
     1921 attcggtcgg gtcgagcttc ttcatcccga cgtggggcac cttgcagaac atggcgatgt
     1981 cgtccgggtc gttctcctcg atcgcctggt tgcagcggcc gttgttgtcg gggttgcccc
     2041 gctcgcgctc cttgcgctcc tcgaccgccc ggtcgaccgc ccgttgcagc ggcgtcatct
     2101 gctcgtcgaa ctcgaccttc ggccggttgg cctgcggcgg cggcgcggcg ccgagtggtg
     2161 ccgcgacgag cagcgccagc gtcagcgtcg agatcaggct gaaattgacg gaacggatcg
     2221 acatggtgcg gacctccacc cggtgcgggg acaatcgcct cgaaatcgcg tcctcgatcg
     2281 gggaagcgac ggccgggcga agatcccctc acggcgctcg tcgggcgagc cggggtgggt
     2341 ccgccgctac acggcccgcg ccagccgccg gtcgtccgct tcggcgtcgg ccggccgctc
     2401 ggcggtcgga tgccggcggg cgaccagcac gtagatcgag ggcacgacga acagggtgaa
     2461 gatcgatccg atgaccatgc cgctcaccag catgatgccg atgctgttgc gcgcgcccgc
     2521 gccgggcccc gacgccagca cgagcggcgt gtgtccgacc accgtcgccg ccgtcgtcat
     2581 caggatcggc cgcagccgcg tcgccgccgc ctcgcgcacc gcgttcacct tgtcgagacc
     2641 ccgcgcctgg agctggttgg cgaactcgac gatcaggatg ccgttcttgg cgatcaggcc
     2701 gaccagcgtg atcaagccga cctggctgta gatgttgagc gtcgtcaacc cgaggaacga
     2761 gaacatcaac gagccggcga tggcgagcgg gaccgacccg gcgaggatga tgaacggatc
     2821 gcggaagctc tcgaactgcg ccgccagcac gagatagatc agcaccgccg acagcaggaa
     2881 cgtcgcgagg aacttgcgac cttcggtgcg aagctggcgc gactgcccgg cgtggtcgag
     2941 gctgaagccg gacggcagca tcgcctgcgc ttcccgctcg aggtagctca gcgcttggtc
     3001 gagcgagacg ttgggtggga tcacgccctg gatccgcacg gcgttgagct gctggaaccg
     3061 cttcagctcg cgcggcacgg tcgtcgtgcg cagggtggcg aaggtcgaca gcggaaccag
     3121 ccgtccgtcg ggtccgctga cgtggatctg ctcgagctga tcgggcgtca accgctcggc
     3181 ccgcttgatc tgcgggatca ccttgtagct gcgcccctgg atgctgaagc gattgacgta
     3241 gtcgcccgac agcatcgtcg ccaggtccga cccggcctgg ctcaggtcga cgccctgcga
     3301 gcgcagcttg tcgcggttga acacgacctc ggcctgcggc tggtcgaact tgaggtccga
     3361 gtcggcgaac atgaacagac cgctctggaa cgccttgacc acgagctggt cggcgatctc
     3421 ggcgagttgc tcgggctcgg cggtcgacgc gacgacgaga tcgatcgggt actggccgcc
     3481 gccgggcagc gcgggcggcg tgagcgggat cacccgcacc gccgggatct tggccagacc
     3541 ggccgcgacc tggagctgcg cctgttgcga ggtgcgctcg cgctcgctcc acggtttgag
     3601 caccacgccg gaaaaccctc cgctcggaaa cgtgatctgg aaggtgtggt tggcctcggg
     3661 aatcgactcg tagacctgat ggacctgcga gatgaacagc cgcgtctggt ccagcgtcgc
     3721 gttcggtgcc gcctgcacga tgccgaacac cacgccctgg tcctcggcgg gcgccagctc
     3781 cttcatcgac aacatgtaga acggcaggct gagcagcgcg acgagcaccc acagcgtcag
     3841 cacgaccgga cgccattcga gcgtccggtc gagcacgcgg gcgtacagtc ggcgcacgac
     3901 ctcgaagtgg cggttgatca tgccggcgaa tccgcgctcg gtatccccgg cgcgcaacag
     3961 cctggagccc atcatcggcg acagcgtcag cgcgacgacg cccgagacga tgacggcgcc
     4021 ggcgagcgtg aaggcgaact cgcggaacag cgcgccggtc aggccgccct gcagcgcgat
     4081 cggggtgtag accgccgcca gcgtgatcgt catcgcgatg atcgggccga ccagctcgcg
     4141 cgcgctcaca agcgccgctt cgatgcgcga tttgcccatg cgcacatgcc gctcgacgtt
     4201 ctccaccacc acgatcgcat cgtccacgac aatcccgatc gccagcacca gcccaaagag
     4261 cgtcagcgtg ttgatcgaga accccagcag catcatcccc gcgaacgtgc cgatcaggga
     4321 caccggcacc gcaag
//
LOCUS       DASPGN010000264         5741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_514188, whole genome shotgun sequence.
ACCESSION   DASPGN010000264 DASPGN010000000
VERSION     DASPGN010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514188"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VD788_07770"
     CDS             complement(<1..890)
                     /locus_tag="VD788_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXV76203.1"
                     /translation="MAAESLSRRPVAALAAGAALISFAPILVKAAGGSGLAPTAIALW
                     RCLLGATILVAAAAVRRQPLDTPREVRGPLLAAGLAFAVDLFVWHRSIVLAGAGMATI
                     LGATQVFGTATLSALLFRERLTPRFVLAAVAAMAGVVLLVGVGSRIEFTADYVAGVAY
                     GLATGLAYAVFLVCLRTAGRRSRGGSTMVRLAWLSIYASAALGVMLGAEGAAIWPDDA
                     ATWVYLALLALIAQSLGWWTISKSLTHVEGATGGLVLLMQPVLATVWGVLLFGERLEP
                     LQILGAAITLAAIAVGSAGDR"
     gene            complement(957..3416)
                     /locus_tag="VD788_07775"
     CDS             complement(957..3416)
                     /locus_tag="VD788_07775"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase"
                     /protein_id="HXV76204.1"
                     /translation="MSRSFSAIACTALPIVVVLALGVAPWAAQVTGDPFPAEIPPDDS
                     EPIGFKKPGTVECPAGAVPAFEGSTTCVIDPAVIPKYVLPLVFPPVMHDSGLAGDYEI
                     AVRQFKQQILPGGHWNFVTGRADAYPATTVWSYGPASDPRPDSSAIPGGAAGVAPAPN
                     SQFNYPAYTVETISYQTVDVRWINDLVDENGRFLKHLVPIDQTLHWANPQRECADGIP
                     RTDCSGTSPDPYGGPVPIVTHVHGAHVGPESDGYPEAWWLPVASNLPQGFSTAGRLFD
                     DVSGMNTGNLGYADFTYPNSQPATTLWYHDHTLGMTRANVYAGPAGFWLVRGGAHDGG
                     VDVSTGMPAVLPGPAPAPGEQVLDLNVPGSAVRAKIREIPVAIQDRTFFPDGSLFYPD
                     NRAFFEGLDAGQLMIDFAPESDVAPIWQPEAFFNVIVVNGVSWPMLEVAQDRYRLRLL
                     NGCNSRFLNLSLHVLGAGGMPVSEIPFYQIGAEQGFLPRVVRIRTGEHVALVPGAPEP
                     LPSSDPDDPTALLLGLAERADVIVDFSSLADGTVVRMINTAPDAPFGGFPDEAADPET
                     TGQILQFVIRAALTGAAGSTDGTTTNPANLLLNAEQPLGAADATRQVSLNEEESSEVC
                     VAIDAATGEFLLDGRGRLRQLRRVMPGEDFEEECERAGGAPFGPVAALLGTVSFDAEG
                     EEAEGMPLMWTDHSGVSQPVTVYKVDGTPVTIHVTENPTVGATEEWEIYNFTADAHPI
                     HLHLVRFQVVDRRLFDGSPSPNGSPRPWEAGFKDTVLAFPGEITTIRAKFDVAGLYVW
                     HCHIVEHEDNEMMRPYVVSDP"
     gene            3780..5174
                     /locus_tag="VD788_07780"
     CDS             3780..5174
                     /locus_tag="VD788_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXV76205.1"
                     /translation="MQDSRFASIVRRTGLVVSVCAFVGASSPSTLAASDPSTGELFIL
                     WDPPSDRSAIDGYRVYYSQTSRDYSGTSFVEVADNRIGGVRVFGIDDCTDWHFVVRSY
                     NETRESRDSNEVFSWPRPRFTSISLPNTIDRGQSKQFELTGASFKDGDRCEVAGMQCQ
                     LTVVDCRTAVANVTALSTTPIGPYALAVTHVDSGVSGVLEGALQVLDPDLPPGHPDNL
                     TDVTPPTIGAVLPRAGSSTVVLTAHPTVEFTEAVFLDDAVIELTDSNGLYVPIELRRL
                     DGRTLEIVPQDLLDYATTYTLRISEIRDVAGNVQQDTLIRSFTTEADPATTVLGTPFA
                     SIAGPTNDDVVWSPGAGAQYYEVWRCIGANCWVAQNTETHFPSVVDRSQALEGVHHVG
                     LDGEFLPPGVAVLEFVNSGLEYTYKVRACKSVDSVELCGAFSNELYYTGRQFMCTESG
                     HEIPCYPDAPLATD"
     gene            5270..>5741
                     /locus_tag="VD788_07785"
     CDS             5270..>5741
                     /locus_tag="VD788_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXV76206.1"
                     /translation="MDQKAVVHMLAEIAETAAETLELQQVFDRVATSIRGVLPFDNMG
                     VVRIVDCDRAVVHATTVRAEGCACVAEHCEPIPLDSWSPRLRPRPGPIPRVDDAETEL
                     DRSFPIDADILAGGVRSTLWEPFTPKDGFKGGLWLSSYRAHAFTDEHQELLRPIA"
BASE COUNT          913 a         2014 c         1941 g          873 t
ORIGIN      
        1 cggtcgccgg cggaaccgac cgcgatcgcg gccagcgtga ttgccgctcc gagaatctgc
       61 agcggctcga gcctctcgcc gaacagcagc acgccccaca ccgtcgccag caccggttgc
      121 atcagcagca cgaggccgcc ggtcgcgccc tcgacgtgcg tcagcgattt cgagatcgtc
      181 caccaaccga gcgactgcgc gatcagcgcg agcaacgcga ggtagaccca ggtcgccgcg
      241 tcgtcgggcc agatcgccgc gccctcggcg cccagcatca cgcccagcgc ggccgaggcg
      301 tagatcgaca gccaggccag ccgcaccatc gtcgagccgc cacgcgaccg gcgcccggcc
      361 gtgcgcaggc agacgagaaa taccgcgtag gccagccccg tcgccaggcc gtaggcgacg
      421 ccggcgacgt agtccgcggt gaactcgatc cggctgccga cgccgacgag cagcacgaca
      481 ccggccatcg ccgcgaccgc ggccagcacg aatcgcggag tcagccgttc gcgaaagagc
      541 agcgcgctga gcgtggcggt accgaagacc tgggtcgccc cgaggatcgt cgccatcccc
      601 gcgccggcca gcacgatcga tcgatgccag acgaacaggt cgacggcgaa ggccaggccc
      661 gccgcgagca gcggaccccg cacctcgcgc ggggtgtcga gcggctgtcg gcggacggcg
      721 gcggcggcga cgagaatcgt cgcgccgagc aggcagcgcc acaaggcgat cgccgtcggg
      781 gcgagccccg agccgcccgc ggccttgacc aggatcggcg cgaagctgat cagcgcggcg
      841 ccggctgcca gcgctgcgac cggtcgtcga ctgagcgatt ccgccgccat ctgccggaac
      901 agggtcgcac aaacacccgc cgccgccggc gccgaagacg cggattcggg tcagcctcag
      961 gggtcggaga cgacgtaggg tcgcatcatc tcattgtcct cgtgctcgac gatgtggcag
     1021 tgccagacgt agagccccgc gacgtcgaac ttggcccgga tcgtggtgat ctcgccggga
     1081 aacgcgagca cggtgtcctt gaagccggcc tcccacggac gcgggctgcc gttcgggctc
     1141 ggactgccgt cgaacagccg gcggtcgacg acctggaacc gcaccagatg caggtggatc
     1201 ggatgggcat cggcggtgaa gttgtagatc tcccactcct cggtcgcccc gacggtcggg
     1261 ttctcggtga cgtggatcgt gaccggcgtg ccgtcgacct tgtagaccgt caccggctga
     1321 ctgactcccg agtggtcggt ccacatcagc ggcatgccct cggcctcctc gccctcggcg
     1381 tcgaaggaga ccgtgccgag caacgccgcg accggaccga acggagcgcc accggcgcgc
     1441 tcgcactcct cctcgaagtc ctcgcccggc atcacgcgac ggagctgccg caagcggccg
     1501 cggccgtcga gcaggaactc gccggtcgcc gcgtcgatcg ccacacagac ctcgctcgac
     1561 tcttcctcgt tcagcgagac ctggcgtgtc gcatccgccg cgcccagcgg ttgctcggcg
     1621 ttgagcaaca ggttggccgg gttcgtcgtc gtgccgtcgg tcgagccggc ggccccggtc
     1681 agtgcggcgc ggatgacgaa ctgcaggatc tgcccggtgg tctccggatc ggccgcctcg
     1741 tccgggaatc cgccgaacgg agcgtcgggc gcggtgttga tcatccgcac caccgtcccg
     1801 tcggccaggc tgctgaagtc gacgatcacg tcggctcgct cggccagccc cagcagcagc
     1861 gcggtcggat cgtcggggtc ggacgacggc agcggctcgg gggcgccggg cacgagcgcg
     1921 acgtgctcgc cggtgcggat gcgcacgacg cgcggcagga agccctgctc tgcgccgatc
     1981 tgatagaacg ggatctcgct gaccggcatc cccccggccc cgagcacgtg caacgacaga
     2041 ttgaggaagc gcgaattgca gccgttcagc agacgcagtc ggtagcgatc ctgcgcgacc
     2101 tcgagcatcg gccagctcac gccgttgacc acgatcacgt tgaagaacgc ctcgggctgc
     2161 cagatcgggg ccacgtcgct ctcgggagcg aagtcgatca tcaactgtcc ggcgtcgaga
     2221 ccctcgaaga acgcgcgatt gtcgggatag aacaacgatc cgtcggggaa gaaggtgcga
     2281 tcctgaatcg cgaccgggat ctcgcggatc ttcgcccgga ccgcgctgcc cggcacgttg
     2341 agatcgagca cctgctctcc gggcgccggg gccgggccgg gaaggacggc gggcattccg
     2401 gtcgacacgt cgacgccacc gtcgtgggcg ccgccgcgca cgagccagaa cccggcgggt
     2461 ccggcgtaga cgttggcccg cgtcatcccc agcgtgtggt cgtgatacca cagcgtcgtc
     2521 gcgggctggc tgttgggata ggtgaaatcg gcgtagccca ggttgccggt gttcatcccg
     2581 ctgacgtcgt cgaacagtcg ccccgcggtg ctgaagccct gcggcaggtt gctcgcgacg
     2641 ggaagccacc aggcttccgg gtatccgtcg ctctccggcc cgacgtgcgc tccgtgcacg
     2701 tgcgtcacga tcggcaccgg cccgccgtag ggatccggcg acgtcccgct gcagtcggtg
     2761 cgcggaatcc cgtcggcgca ctctcgctgc gggttcgccc aatgcagcgt ctgatcgatc
     2821 ggcaccagat gcttgaggaa ccgaccgttc tcgtcgacca gatcgttgat ccagcgcacg
     2881 tcgacggtct ggtaggagat cgtctcgacg gtgtacgccg ggtagttgaa ctgcgagttc
     2941 ggtgccgggg ccacgccggc ggcgccaccg gggatggccg aggaatcggg ccgcggatcc
     3001 gaggccggtc cgtagctcca aaccgtcgtc gccgggtagg cgtccgctct gccggtgacg
     3061 aagttccagt gcccgcccgg cagaatctgc tgcttgaact gacgcacggc gatctcgtag
     3121 tcgccggcga ggccgctatc gtgcatcacc ggcgggaaga cgagcgggag cacgtacttc
     3181 ggaataacgg ccgggtcgat gacacaggtg gtcgagccct cgaaagcggg gaccgcgccc
     3241 gcgggacact cgacggtgcc gggtttcttg aagccgatcg gctccgagtc gtcgggcgga
     3301 atctcggccg gaaacgggtc gccggtcacc tgcgcggccc acggcgcgac accgagggcc
     3361 agtacgacga cgatcggcaa cgccgtgcac gcgatcgcgc tgaacgaacg tgacatgatc
     3421 gaacccccca agacgtcgcc gtgcgacgcg acggtccgcg tcgggcgacg ccatctccca
     3481 ggggtatagt ccgtcgacgc gcgaggctcg accctcgact ccggacaatc gagagcgaca
     3541 aggtctcgaa tgcgcacgtg cctcgatcgc gaacccgcgc gttgaccaac ccgcgagcag
     3601 tgcgcatcgt gccatgcgct ggcgtgattc gaccgcttgc ggtcgtacgg tccgcaagtc
     3661 gccgagcgcg gtgtgtttcg tcgtcggcaa gcgtgtcttc ggccccacgc gtttcacgct
     3721 cgcttgccgg cgttgttccc attcgtcgcg tggcaccgtt cttgccattc gcggtcgcca
     3781 tgcaggacag tcgcttcgct tcgatcgtcc gccgcacggg gctcgtcgta tcggtctgcg
     3841 ccttcgtcgg cgcatcgagc ccgtcgactc ttgccgcgag cgatccgtcg accggtgagc
     3901 tgttcatcct gtgggatccg ccgagcgacc gatcggcgat cgacggctac cgggtctact
     3961 actcgcagac gtcgagagac tattcgggta cgtcgttcgt cgaggttgcc gacaaccgga
     4021 tcggcggcgt ccgcgtcttc ggcatcgacg attgcaccga ctggcacttc gtcgtgcgct
     4081 cgtacaacga gacccgcgaa tcgcgcgatt cgaacgaggt cttcagctgg ccgagacccc
     4141 gattcacctc gatctcgctg cccaacacga tcgaccgcgg gcagtcgaag cagttcgaac
     4201 tgaccggggc cagtttcaag gacggggatc gctgcgaggt cgccggcatg cagtgtcagc
     4261 tgacggtcgt cgactgccga acggcggtgg cgaacgtgac ggcgctgtcg acgaccccga
     4321 tcggcccgta cgcgctcgcg gtgacccacg tcgactccgg agtttccggc gtgctcgagg
     4381 gtgcgctgca ggtgttggac ccggatcttc cgcccggcca ccccgacaac ctgacggacg
     4441 tgaccccgcc gacgatcggg gcggttcttc cgcgcgccgg gtcgtcgacg gtcgtcttga
     4501 ccgcgcaccc gaccgtcgag tttaccgaag ccgtcttcct cgacgacgcc gtgatcgagc
     4561 tgaccgattc gaacggcctg tacgttccga tcgagctgcg tcggctcgac ggccggacgc
     4621 tcgagatcgt gccgcaggac ctgctcgact acgccacgac ctacacgctg cggatcagtg
     4681 agatccgcga cgtcgccggc aacgtgcagc aggatacgct gatccggtcg ttcaccaccg
     4741 aagccgaccc cgcgaccacg gtgctcggca cgccgttcgc ctcgatcgcc ggcccgacca
     4801 acgacgacgt cgtctggtcg ccgggcgcgg gcgcacagta ctacgaggtc tggcgctgta
     4861 tcggcgcgaa ctgctgggtg gcacagaaca ccgagaccca cttcccgtcg gtcgtcgatc
     4921 ggtcgcaagc gctcgagggt gtgcaccacg tcggtctgga cggagagttc ctgccgcccg
     4981 gtgtggccgt cctcgagttc gtcaactcgg ggctcgaata cacctacaag gtccgggcct
     5041 gcaagtccgt cgattcggtc gagctctgcg gcgcgttctc gaacgagctg tactacaccg
     5101 gccggcagtt catgtgcacg gaatccggac acgagatccc gtgctacccg gacgctccgc
     5161 tcgccaccga ctgagcgccc tcggcccact cgttctgcaa gtggcctccg accctgtcgg
     5221 ctcgccggtt cggccgaatt cggccggccg ggctacaatg ctccgaacca tggatcaaaa
     5281 ggccgtcgtc cacatgctgg cggagatcgc cgagaccgcc gcggagacgc tcgagctgca
     5341 gcaggtcttc gaccgcgtgg cgacgtcgat ccgcggcgtc ctgcccttcg acaacatggg
     5401 ggtcgtgcgc atcgtcgact gtgaccgggc ggtggtccac gcgaccacgg tccgcgccga
     5461 gggctgcgcc tgcgtcgccg agcactgcga accgatcccg ctcgactcct ggtcgccgcg
     5521 gctgcggccg cgccccggac cgatcccgcg cgtcgacgat gccgagaccg agctcgaccg
     5581 gtcgttcccg atcgacgccg acatcctcgc cggcggcgta cgatcgacgc tgtgggagcc
     5641 gttcacgccg aaggacggct tcaagggcgg gctgtggctc agctcctacc gggcgcacgc
     5701 gttcaccgac gagcaccaag agctgctgcg gccgatcgcc g
//
LOCUS       DASPGN010000265         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1216804, whole genome shotgun sequence.
ACCESSION   DASPGN010000265 DASPGN010000000
VERSION     DASPGN010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216804"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VD788_07790"
     CDS             complement(<1..528)
                     /locus_tag="VD788_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HXV76207.1"
                     /translation="MSPTDPGREPGVVSLRIDGLAAGGRGVGRHEGRVWFVERGLPGD
                     RVLARPIARRARFVEAERVRLERPSPSRRQPPCVHQDRCGGCPWMPLAEVEQRAWKER
                     LVRDALARIGGIADCPVEPTRSTGSPLGYRNRVEFAVAWDESGRATIGLHRAGGGGLV
                     AIDRCLLQSDAANRTL"
     gene            complement(1213..2388)
                     /gene="aroC"
                     /locus_tag="VD788_07795"
     CDS             complement(1213..2388)
                     /gene="aroC"
                     /locus_tag="VD788_07795"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXV76208.1"
                     /translation="MALRFLTAGESHGPALTVVLEGLPAGVLVDLDRVHAEMRRRMGG
                     YGRGGRMAIETDRVEIVGGVRFSRTLGSPVSLVIRNLDHSNWTDAMPVHGPHPGPGPA
                     RSVSRPRPGHADLAGALKYDTHDARDVFERSSARETAARTAAGAIAKSMLREAGIEVT
                     SHTLGVGRARAPALADDAFDALLALADDAPMRTIDPAAAAAMIEAVELARRRKDSIGG
                     VFEVLARGVPPGLGTHAHWDRRLDARLGGALLSIQAVKGVSVGEGVESAARHGSEQHD
                     EIGYDAAHGRFVRPTNRAGGIEGGMSNGEMIRLRGYLKPLATLPRPLRTVDLVTKQPL
                     DAVRERTDTIPIVAAGVVGEGMTALVLADELLIKFGGDSVGELLRNLESYREQLRRF"
     gene            2503..>2696
                     /locus_tag="VD788_07800"
     CDS             2503..>2696
                     /locus_tag="VD788_07800"
                     /inference="COORDINATES: protein motif:HMM:NF015791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HXV76209.1"
                     /translation="MEILTGAEMRRIDRRAIDGRGIDGLLLMEAAGRGVADALCRDHP
                     ALARDGVLVVAGKGNNGGDG"
BASE COUNT          405 a          959 c          915 g          417 t
ORIGIN      
        1 gagcgtgcgg ttggccgcgt ccgattgcag cagacagcgg tcgatggcga cgagcccgcc
       61 gccacccgcg cggtggaggc cgatcgtcgc gcgcccgctc tcgtcccagg cgacggcgaa
      121 ctcgacgcgg ttgcgatagc cgagcggcga gccggtcgac cgagtcggtt cgaccgggca
      181 gtcggcgatt ccgccgattc gcgcgagcgc gtcgcggacg agacgctcct tccatgcgcg
      241 ttgttccacc tcggccaatg gcatccatgg acagccccca cagcggtcct gatggacaca
      301 gggcggctgc cgccgcgagg gcgacggtcg ctccaaccgg acccgctccg cctcgacgaa
      361 gcgcgcgcgc cgagcgatcg gtcgagcgag gacccggtct ccgggaaggc cgcgttcgac
      421 gaaccagacg cggccttcgt gccgcccgac cccgcgcccc ccggcggcga gcccgtcgat
      481 tcgcagggag acgacccccg gttccctgcc cgggtccgtg gggctcaccc ccatatcctg
      541 gggctccgga aaactaatat tcgatatgtt ggacttttcg cttgacatcg cctgtcggct
      601 ccggctatcg tccgcgagaa tcggttcgac gtctcggcca ccagccgggg cgtcaggacg
      661 agtgctacgg gtgaaagaaa gggtttggat cgtgtcgttc gggcgctgct cgctctctcc
      721 ggcggattgc aacccgacct caatgcccag gaggccggct tcggagccgt ctctctcgcc
      781 taccacttgt cggaaacggc aagtatcccg actacccacc gttggagtct tgacccgtgc
      841 cagggtggcc gttcaccgga gagagcgacc agcgtcggaa tgaatataac accgtagcgc
      901 gggcgtcaac cgggatgtga cgccgcgccg gtctttgtcg gccggacacc gccgacaaag
      961 tgccctttcc cgtgagatat cgcctttaca gccccagcgg gccgccgtat gttcatggca
     1021 ccctcgacga ggacgaacat tcggaaaagc tggggacccg ccagggcagg ccgctatgac
     1081 cgggggggga ttggatcggc cgcctggcgg tcccgagttg tgtaagtcga gtagcccacc
     1141 tccccgacga ggggagtact cgatttgccc gatttgacgg gcggcgacgc gttcgccgcc
     1201 cgtttttttt gctcagaatc gtcggagttg ctcgcgatac gattcgaggt tgcgcagcag
     1261 ctccccgacc gaatcgccgc cgaacttgat cagcaactcg tcggccagga ccagcgccgt
     1321 catcccctcc ccgaccacgc ccgcagcgac gatcgggatc gtgtcggtcc gctcgcgcac
     1381 ggcatcgagc ggctgcttcg ttacgagatc gaccgtgcgc agcgggcgcg gcagtgtcgc
     1441 cagcggcttc aggtaaccgc gcaggcggat catctcgccg ttcgacatcc cgccttcaat
     1501 gccgccggcc cggttggtcg gacggacgaa acgtccgtgc gcggcgtcgt agccgatctc
     1561 gtcgtgctgc tcgctgccgt gccgcgcggc cgactcgaca ccctcgccga cgctgactcc
     1621 cttgacggcc tggatcgaca gcagcgcgcc gccgagacgt gcgtccaggc gccggtccca
     1681 gtgtgcgtgt gtccccagtc cgggtggcac accgcgagcc agcacttcga aaaccccgcc
     1741 gatcgagtcc ttgcggcgcc gggccagctc gacggcctcg atcatcgccg ccgccgccgc
     1801 cgggtcgatc gtgcgcatcg gcgcgtcgtc cgccagcgcg agcaacgcgt cgaacgcgtc
     1861 gtcggccagt gccggtgccc gggcgcgacc cacgcccagc gtgtggctcg tcacctcgat
     1921 ccccgcctcg cgcagcatcg atttggcgat cgcgccggcc gccgtcctgg ccgccgtctc
     1981 gcgcgcgctc gagcgctcga acacgtcgcg cgcgtcgtgc gtatcgtact tgagcgcgcc
     2041 ggcgaggtcg gcgtgacccg gacggggacg cgagacgctc cgcgccggtc cgggccccgg
     2101 gtgggggccg tggaccggca tcgcgtcggt ccagttcgag tggtcgagat tgcgaatcac
     2161 gagcgagacc ggcgatccca gcgtgcgaga gaagcggacc ccgccgacga tctcgacccg
     2221 atcggtctcg atcgccatcc ggccgccgcg accgtagccg cccatccgcc gccgcatctc
     2281 ggcgtgcacc cggtcgaggt cgacgagcac gccggcgggc agaccctcga gtacgacggt
     2341 cagcgccggc ccgtgggatt cgccggcggt caggaaacgc aacgccatcg ctcgatactc
     2401 cgaatcgtgg attgtaaccc gtttgtcgcc gggttgaccg cgcgcgcgcg gccggactat
     2461 cttgccgctc cggcgcgacg tgtcgacgag aaagaaccga cgatggagat cctgacgggc
     2521 gccgagatgc ggcgcatcga ccgccgagcg atcgacgggc gcggcatcga cggcctgctg
     2581 ctgatggaag ccgccggacg cggtgtggcg gatgcgctgt gccgcgatca cccggcgctc
     2641 gcccgcgacg gcgtgctggt cgtcgccggc aagggcaaca acggcggcga cggctt
//
LOCUS       DASPGN010000266         9832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_281543, whole genome shotgun sequence.
ACCESSION   DASPGN010000266 DASPGN010000000
VERSION     DASPGN010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281543"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..1199
                     /locus_tag="VD788_07805"
     CDS             411..1199
                     /locus_tag="VD788_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76210.1"
                     /translation="MTRRTRFRLTVLAIASTLVTPLYASRLPDSYLALPVLLDDDASG
                     VYLQHRQSVYLVTARHVLFGPEGGEPTRPRVTLRSFTENRSIPDPIVFELDLAALYES
                     HHILSSKADDIAAVRIGDVTSQEDHTVHWESAPGVVARAQPGPAQIILGANEWLRFDE
                     AKVGNDVFLASTEQLERGRPLLLEGTLAEKQRATRRLIVDVRVSPRNLGGPAIAIEEV
                     GPRSRQPRFVGIVVERIRQAPSFDDSEHAVVASVDAILDLIEKP"
     gene            complement(1213..4185)
                     /locus_tag="VD788_07810"
     CDS             complement(1213..4185)
                     /locus_tag="VD788_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELWxxDGT repeat protein"
                     /protein_id="HXV76211.1"
                     /translation="MRCRIFGWSALVGLSQLLGSVAVLAQTDPARLVKDINTSPTSSS
                     GSSPVGFVELGGFLYFKAFTAANGTELWKSDGTEAGTVLVKDIRPGIVGSDPREFVAV
                     GDTLFFFANGGQGEELWKSDGTEAGTVLVKDIRPGPSSSLPLNFTRVPTAAGGMLFFG
                     ANDGVHGDELWRSDGTGDGTFLLKDIRPGNVGSSPGGLIEVDGGVLFAVDDGTHGHEL
                     WKSDGTQGGTDLMIDLEPGSRGSSPRVIARFGTFVYFTGSFGDYPLHSALMKTDGTAA
                     GTSVVTSLPRLFSSTGIVYQGALYITARTFTSNLQLWKSNGSAAGTVLVKEFPVVPNF
                     GDVGGLGGFTEANGELYFVMHDGNGEYIELWKTRGTGATTTRVKVLDAGGDGTWHYTL
                     DLIAVNGGLVFRSFDHEHGFELWRTDGTAAGTVLLKDIRLGLPYSLPFSFAAINGLVL
                     FSANDGIHLTELWRTDGTAAGTALLKDINSVIFTASSSPNGGVEFGGMHFFNASAGTG
                     SELWKSDGTEAGTVLVKDIRPGPSGSAPAGLTVVDGTLFFVADDGVHGREPWKTDGTE
                     AGTVLLRDVRPGAQSSSPGLLNEAYGLLFFAGDDGIHGSELWKSDGTEAGTVLVKDIN
                     PGPAPAHPLELPGENPTLYFDADDGIHGFELWKSDGTEAGTVLVKDINPGPASSFSGR
                     AVEVGGVFVFDASDGVHGRELWRTDGTEAGTQLVIDIWPGSASAFFGRLEPFDGNLYF
                     SGDDGIAGAELWKTDGTQAGTQLVHDIWPGEFGSSIDRLTAFDGTLFFVASDDIAGTE
                     LWKSDGTEAGTQLVEDIHQGMFGSSPRDFVRAGGVLLFTADDDVTDRELWRSDGTEDG
                     TALVQDIGPGGLTSNPSNLVVAGSRLFFAANELVTGRELWAGRAAILARRPDRAIRDL
                     GDEVRALGLHPGIERSLIAKLDAAAAALDRGEGPAIYLLEAFVREVESHDPAVIPEPA
                     AANLLEFAREIVDLLEAR"
     gene            5213..5548
                     /locus_tag="VD788_07815"
     CDS             5213..5548
                     /locus_tag="VD788_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76212.1"
                     /translation="MLRGRVQSSRDRIRVNVQLFDGRDGRSVWGNTFESEQSARDLFD
                     LQVELTQQVVNAIAGAHGVLTRAELPHTRRKPPESLASHDCVFRADGYLLDDPLQAFT
                     RRQMGLGRV"
     gene            5594..6115
                     /locus_tag="VD788_07820"
     CDS             5594..6115
                     /locus_tag="VD788_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76213.1"
                     /translation="MALDNGNVLVHTALGLAALFSGEVEQGLAELRRAAELRPHNLIV
                     LTLLANYLANQGEFELALSLTRRVIELNPHPPSWVAYPMFADHYLHGRYDAALELARQ
                     DLMGSGDFRDPLFLAPRSGSSAGPTKPRPRSATCVRPGSRLIERHAFSALFTDELIEG
                     LEQAGLDEPDSRR"
     gene            complement(6207..7181)
                     /locus_tag="VD788_07825"
     CDS             complement(6207..7181)
                     /locus_tag="VD788_07825"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXV76214.1"
                     /translation="MGRYECVVIGGGPAGLQAALLMARGGTRAIVYDSGTARHLASAE
                     SHSFLAADGMPPAELRRRGREQLKPYGTVGFRDATVERVEPRLEGGFRVSIAGENDAV
                     TTRFVLLATGVRDVPLAIPGFDDFWGTSILHCPYCHGWESRGLELGVLLFDEEMVDLA
                     HKVTWWSRDVIVFVSPDLDLRPESSARLRKLDVAIETRRVRRFVGDAKARRLDAIELW
                     DGTLVPRAAVYYRPKQRQAALVEALGSSLDLRLDAGGLVQVDDGQQTSVPGLYAAGDL
                     TTAYQQIAEAARQGHRAASSIEHALKVDGRPADAAASGPWARDGDDLG"
     gene            complement(7339..>9832)
                     /locus_tag="VD788_07830"
     CDS             complement(7339..>9832)
                     /locus_tag="VD788_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76215.1"
                     /translation="LDFGLAKLTDESAPAAGTQLGTVTAATTHGAILGTVCYMSPEQA
                     EGLAVDARSDVFSLGTMLYEMATGRLPFAGQTPISTITAILRDAPPPVSELRDAIPAA
                     LDAVITRCLEKDPDRRFQDAGALRDALRSVGAGEAARPRRNGTRLALGAIAAAVVVAL
                     AVSGWTWLRRGESGGAWPGAPDQLTAGRTVAVMGFENVTDPDDAEKLGRVLMGLVTTD
                     LVESRGLDVLSTARVLAAIREVGGAVDRGFDLSVANRAAIEAGAELMLVGQVNRDGER
                     ILLAAELVDVRTGKTTGSIRREAGSASELFELAGSIAAEIRRQLGVSTSGDGAAPFDL
                     AESLTSSAEAYRRYAAGELELHLGRFDAAGRHFEAAIGHDPAFALAYFRLGMTRSWAG
                     HREASREALEQGLPHVERLPDDWQRVYRAYVDYGRGRWAEAHDALDALADEQVPIADL
                     YYVLSEIVTHASRYWDPRRVRFLSERALELDPTFATAWFHLLQVYALADDRGALRDAV
                     ERLRAQKVDHVEILRTELNRLAAERRFDEMEPVFREMGPGSVTMTHVMVQGWLRLGQD
                     QRAVDACRAAAGDPDVDEATLSHTLAVTQAWRGRFDEADASLAAARSSGAAHAISTYT
                     LASVRIARLTGDPTRAMREAEEAVEIDRENGAAWYWLGRVQIERGDRASAERSLDGLR
                     AIAASVPSPLGAFWVGLLAAELELARGATGQALATLDRIASLPAEHRDRPLEMLVRAR
                     ALAAAGDTAGAIAAYRVLLDPPYLLAGEHGTPWLDWPLYEAELLYPLALLESADGQTE
                     PAREHLRAYVEHWAGADRPVVGIDDARARLGG"
BASE COUNT         1517 a         3501 c         3332 g         1482 t
ORIGIN      
        1 ccggcccgtg ccccttccgc tcgccgggcc ggtcgtcggc gccctcgacc ccaccggcct
       61 cgacggcgag accgagcacg cagaccgggc agctctcctc gcccggcgcg ccgcgcagcg
      121 ccgtaccgca gcgagggcag cgcgtctcgg tcatcggcgt cctccgcttc ccgacggcag
      181 gttgttcggg cggtgttccg gattctatgc cgcgaccccg ccccggtaac ggagagatac
      241 gattctggac gaaaagacaa cgccacccga gtccgtttcg acaaagaaac cggtatccgt
      301 gtttcgcatt cgttgccgga cgacggcgac gcgggccgcc gacgggggcc ggagcccggc
      361 acggcgcttg cagttacggc aagcggccac gctcgctgac gggaggcggt gtgacacggc
      421 gaactcggtt tcgactgacc gtgctggcca tcgcgtcgac gttggtcacg ccgctctacg
      481 catcgcggct accggactcc tacctggcct tgcccgtgct gctcgatgac gacgcgagcg
      541 gcgtctacct gcagcatcga cagtccgtct atctcgtcac cgcccggcac gtgctcttcg
      601 gtccggaggg gggcgagccg acgcggccgc gcgtcacctt gcgctctttc acggagaacc
      661 ggtcgattcc cgatccgatc gtcttcgagc tcgatctcgc ggcgctctac gagtcacacc
      721 acatcctgtc gagcaaggca gatgacattg ccgcggtgcg gatcggggac gtaacgagtc
      781 aggaggacca cacggttcac tgggaaagcg ctcccggggt cgtggcgcgg gcgcagcccg
      841 gtccggctca gatcatcctc ggtgcgaacg agtggctccg gttcgacgag gcgaaggtgg
      901 gcaacgacgt gttccttgcc tcgacggaac agctcgagcg cgggcgtcca ctcctgctcg
      961 agggtaccct tgccgagaag caacgggcta ccaggcggct gatcgtggac gtgcgcgtct
     1021 ctccgaggaa tctcggagga ccggcgatcg cgatcgagga agtcgggcct cggagccggc
     1081 agccgagatt cgtcggtatc gtcgtcgaaa gaatccgcca agcaccgtcc ttcgatgact
     1141 cggagcacgc cgtcgtggct tccgtcgacg cgatcctcga tcttatcgag aagccgtgag
     1201 gacgaaacga gcctaccgag cctcgagcag gtcgacgatc tctcgcgcga actcgaggag
     1261 gttcgctgca gccggttcgg ggatcaccgc cgggtcgtgg ctttcgacct cgcggacgaa
     1321 cgcctcgagc aggtagatcg cggggccctc gccccgatcg agcgcggctg ccgcggcgtc
     1381 gagcttggcg atcaggctcc tctcgatccc ggggtgcagt cccagcgctc ggacctcgtc
     1441 gcccagatcc cgaatcgcgc gatcgggccg ccgagccagg atcgccgccc gcccggccca
     1501 aagctccctt ccggtcacga gctcgttcgc ggcgaagaac agacggctgc ccgcgacgac
     1561 caggttcgac ggattcgacg tgaggccgcc gggtccgatg tcttggacca acgccgtccc
     1621 gtcctccgtt ccgtcgctcc gccacagctc ccggtcggtg acgtcgtcgt cggcggtgaa
     1681 caacaagacc ccgcccgcgc gcacgaaatc gcgcggcgac gagccgaaca taccctgatg
     1741 gatgtcctcg acgagctggg tgccggcctc cgtgccgtcg ctcttccaca gctccgtgcc
     1801 ggcaatgtcg tcgctcgcga cgaagaacaa ggtcccgtcg aacgcggtga gcctgtcgat
     1861 ggacgagccg aactcaccgg gccagatgtc gtggacgagt tgggtgccgg cctgcgtccc
     1921 gtcggttttc cacagctccg caccggcgat accgtcgtcg ccgctgaagt acaggttccc
     1981 gtcgaacggc tcgagacgtc cgaagaacgc gctggcgctg cccggccaga tgtcgatgac
     2041 gagctgcgta ccggcctccg tcccgtcggt tctccacagc tcccgcccgt ggacgccgtc
     2101 gctcgcgtcg aagacgaaga ccccgccgac ctcgacggcg cgaccgctga acgaggacgc
     2161 gggtcccgga ttgatgtcct tgaccaggac ggtgccggcc tccgtgccgt cgctcttcca
     2221 cagctcgaag ccgtggatgc cgtcgtcggc atcgaagtag agggtcgggt tttctccggg
     2281 cagctcgaga gggtgagcag gggcgggacc cgggttgatg tccttgacca ggacggtgcc
     2341 ggcctcggtg ccgtcgctct tccacagctc ggagccgtgg atgccgtcgt caccggcgaa
     2401 gaacagcaga ccgtaggcct cgttgagcag gccgggagac gacgattgcg cgcccggcct
     2461 gacgtccctc aggagaacgg tccccgcctc cgtcccgtcg gtcttccacg gctcgcgacc
     2521 gtggacaccg tcgtcggcga cgaagaacaa ggtcccgtcg accacggtga gcccggccgg
     2581 tgctgacccc gacggacccg ggcggatgtc cttgaccagg accgtgccgg cttccgtccc
     2641 atcgctcttc cacagctccg atccggtgcc ggccgaggcg ttgaagaagt gcattcctcc
     2701 gaactcgacg ccgccgttcg gcgacgagct cgccgtgaag atcaccgagt tgatgtcctt
     2761 gagcaacgcg gttccggccg cggtgccgtc ggtccgccac agctccgtca ggtggatccc
     2821 gtcgttggcg ctgaacagta cgagcccgtt gatcgcggcg aagctgaaag gcagcgagta
     2881 ggggaggccg aggcggatgt ccttgagcag gacggtgccg gccgcggtgc cgtcggtcct
     2941 ccacagctcg aagccgtgct cgtggtcgaa ggagcggaag acgaggcctc cgttcactgc
     3001 gatgagatcg agggtgtagt gccacgtccc gtcgccaccc gcgtcgagaa ccttgacacg
     3061 ggtcgtagtc gctcccgtcc ctcgggtctt ccacagctcg atatactcgc cgttgccgtc
     3121 gtgcatgacg aagtacaact ctccgttggc ctcggtaaat ccgcccaggc ccccgacgtc
     3181 gccgaaattg ggaacgaccg ggaactcctt caccagcacg gtgccggctg ccgacccgtt
     3241 gctcttccag agttggaggt tgctcgtgaa cgttcgtgcc gtgatgtaca gtgcgccctg
     3301 gtagacgatg cccgtggagg agaacagacg gggcagcgac gtcacgaccg aggtacccgc
     3361 cgcggtgccg tcggtcttca tcagcgcgct gtgtagggga tagtccccga agctgccggt
     3421 gaagtagacg aacgtgccga agcgcgcgat tacccggggg gaagagccgc gggatcccgg
     3481 ctcgaggtcg atcatcaggt cggtgccgcc ctgcgtcccg tcgctcttcc acagctcatg
     3541 gccatgggtg ccgtcgtcga cggcgaacag cacgcccccg tcgacctcga tcagcccgcc
     3601 ggggctcgag ccgacgttcc cgggccggat gtccttcagc aggaacgtgc cgtcccccgt
     3661 cccgtcactc ctccacagct cgtccccatg gacgccgtcg ttggccccga agaacagcat
     3721 cccgccggcc gcggtcggga cacgcgtgaa gttgagaggg agcgaactgg agggtcccgg
     3781 tcggatgtcc ttcacgagga cggtaccggc ctcggtgccg tcgctcttcc acaactcttc
     3841 gccttggccg ccattggcga agaaaaacag cgtatcgccg accgcgacga actcgcgcgg
     3901 atcggagccg acgattcccg gtcggatgtc cttcacgagg acggtgccgg cctcggtgcc
     3961 gtcgctcttc cacagctcgg tcccgttggc cgccgtgaag gccttgaagt aaaggaagcc
     4021 gccgagctcg acgaacccga ccggcgagga cccagacgac gaagtcggcg acgtgttgat
     4081 gtccttcacc aaccgcgcgg gatccgtctg cgccaggacg gcgaccgaac cgagaagctg
     4141 cgacaagccg accagcgcac tccagccgaa tatccggcag cgcatcgtcc caccccccac
     4201 gacggcgcat tgcgccgtcc caccaccctc gacgcggctc tgggacgagc cgcgagtatg
     4261 cccgcaaaat tacgccaacc gcgactggcg gtcaatccaa tcgtccgagt gccgtagaat
     4321 ccgtccaaga ccgagcgaac ccgcatccgg gaagtgcacg cggggccgtt ctacgatgaa
     4381 gagcagcgga gtggggatcg gcgccgggca gcagctactg cactaccgcc tgatcgcgaa
     4441 gatcggcgag ggcgggatgg gtgtcgtgtg gaaggccgag gaggcgcgcg cggcgtcggc
     4501 gatcaaccac ccgcacatct gctcgatctt cgacaacggt cggcctcgcc aagctggcgg
     4561 ccggcgcgga cgccacgccc ggtcccgacg acgcgacgcg gacttctctc gacacgacga
     4621 cgcccggttc ggtgctgggg accgtgttct acatgtcccc cgagcaggcg ctgggacggc
     4681 cgctcgacgc gcgcaccgat ctgttctcgg tcggcgtcgt gctgtacgag atgatcaccc
     4741 cccggccgtc gcgcgttcgg cggcacgacg tcggccgcgg tgttcgacgc gatcctcaac
     4801 cgggcaccgc cggcgttgac cgaggtcgac gcccgggtac cgccggagct cgagcggatc
     4861 gtcaaccggt tgatcgagaa ggagcccgcg cggcgctacc ggagcgccgc cgagctggcc
     4921 gcggacctgc gccggctgcg gaacgaatcg atggcgccgg ggccggcggc gccgaaggcc
     4981 gggcgctcca cacgccgacg gaaccggctc gcgctcggtt cgctggccgt gctgctgctg
     5041 atcgtcgccg gtgtctggct gctgcggatg cgcgacgacg aagtcggctc ccccacgctc
     5101 ggcacgggct cctcggtcac cgggctcagg atcgcagtgc tgccgttcgt caacgccagc
     5161 ggcgacccgc agcagcgcta cttcagcgaa ggtctgacct ggcgcgcgct acgtgctgcg
     5221 cgggcgcgtg cagtcctcgc gcgaccgcat ccgggtcaac gtgcagctgt tcgacggccg
     5281 cgacgggcgg tcggtctggg gcaacacgtt cgaaagcgag cagtcggccc gcgacctgtt
     5341 cgacctccag gtcgagctga cccagcaggt ggtcaacgcg atcgccggag cccacggcgt
     5401 gctgacgcgc gccgagctgc cgcatacgcg tcgcaagccc cccgagagcc tggccagtca
     5461 cgactgcgtc ttccgggccg acggctacct tctcgacgac ccgctccagg cattcacgcg
     5521 ccgccagatg ggtctcggcc gtgtgtgagt tctgcaagcg agccaggcta cgtcgacgat
     5581 aggcgagcgc gcagtggccc tcgacaacgg caacgtgctg gtgcacaccg cgctcggact
     5641 ggccgcgctc ttttcggggg aggtcgagca ggggctagcc gagctgcgac gtgccgcgga
     5701 gctccggccg cacaacctga tcgtgctcac gttgttggcc aactacctgg ccaaccaggg
     5761 cgagttcgag ctggcgctgt cgctgacccg gcgggtgatc gagctcaacc cgcacccgcc
     5821 gagctgggtc gcctacccga tgttcgcgga ccactacttg cacggtcgct acgacgccgc
     5881 gctcgagctc gctcggcagg atctgatggg ctccggcgat ttccgcgatc cgctgttcct
     5941 cgcgcctcgc tcgggcagct cggccgggcc gacgaagccg cgcccgcgct cggcgacctg
     6001 cgtgcgacct ggctcgcggc tgatcgagcg ccacgccttc tcggcactct tcactgacga
     6061 gctgatcgag gggctggagc aggccgggtt ggacgagccg gattctcgtc ggtgacaatg
     6121 tagctacgtc cgcactgccc gcacgctcga cgcgatgctc ggcttcgacg gggtgggcaa
     6181 gacaatgccg aggcgctcgg catcgctcag ccgagatcgt cgccgtctcg ggcccacgga
     6241 cccgaagcag cggcatcggc cggtcgcccg tccaccttca gcgcatgctc gatggacgac
     6301 gcggcgcggt gtccctggcg cgcggcctcc gcgatctgct ggtaggcggt cgtcaggtcc
     6361 cccgcggcat acagcccggg aacgctcgtc tgctgcccgt cgtcgacctg tacgaggccg
     6421 ccggcgtcga gccgaaggtc gagggaactg ccgagcgctt ccacgagagc ggcctgccgt
     6481 tgcttcgggc ggtagtagac cgccgcgcgt ggaaccagcg tcccgtccca gagctcgatt
     6541 gcatcgaggc gccgtgcctt cgcatcgccg acgaaacgtc gcactcgtcg tgtctcgatt
     6601 gcgacgtcga gcttgcggag ccgggccgac gattcgggtc gtagatcgag atcgggggag
     6661 acgaacacga tcacgtcgcg cgaccaccag gtcaccttgt gcgcgaggtc gaccatctct
     6721 tcatcgaaca gcaggacgcc gagctcgagc ccgcgagact cccagccgtg gcagtaggga
     6781 caatggagga tgctcgtccc ccagaaatcg tcgaagccgg gaatcgcgag ggggacgtcg
     6841 cggacacccg tggcgagcag gacgaagcgg gtcgtcacgg cgtcgttctc accggcgatc
     6901 gacacgcgaa agcctccctc cagtcgcggt tcgacgcgct cgacggtcgc atcgcggaat
     6961 ccgaccgttc cgtagggctt caactgctcg cgaccccgac gacgaagctc agccgggggc
     7021 ataccgtcgg cggccaggaa gctgtgcgat tcggccgatg cgaggtggcg cgcggttccg
     7081 gaatcgtaga cgatcgcgcg cgttccaccg cgcgccatca gcagcgccgc ctgcagaccc
     7141 gccggacctc cgccgatcac cacgcattcg tatctgccca tcgaacgcac gcaaactcca
     7201 cgccgacgac ttcggtctgt ccttcaagtc cggtttgagt atgcaccgac gcttcaaagt
     7261 acgcaggtcg agctgcgtag ggccgccgtc cggactcgtg gcttcgatcc tccgctccgt
     7321 cgaggcatcg cccttgcctc accccccgag ccgagcgcgc gcgtcgtcga tcccgacgac
     7381 cgggcggtcc gcgccggccc agtgctcgac gtaggcgcgc aagtgctccc gcgcaggctc
     7441 ggtctggccg tcggccgact cgagcagggc cagcgggtag agcagctcgg cctcgtagag
     7501 cggccagtcg agccacggcg tcccgtgctc gcccgccagg aggtagggcg ggtcgagcag
     7561 cacgcgatag gccgcgatcg cgccggccgt gtcgcccgcg gcggcgagcg cccgcgcgcg
     7621 gacgagcatc tcgagcggac gatcacggtg ttcggcgggg agcgaggcga tgcggtcgag
     7681 tgtcgcgaga gcctgcccgg tcgcgccgcg cgccagctcc agctcggcgg ccagcagacc
     7741 gacccagaaa gctccgagcg ggctcgggac gctcgccgcg atcgcgcgca gcccgtcgag
     7801 cgagcgctcg gccgacgccc ggtcgccgcg ctcgatctgc acgcggccga gccagtacca
     7861 cgccgcgccg ttctcgcggt cgatctcgac ggcttcctcc gcctcccgca tggcgcgggt
     7921 cgggtcgccg gtaagccgtg cgattcgcac cgacgccagc gtgtaggtcg atatggcatg
     7981 cgcggcgccg gaagaccggg cagccgcgag gctcgcatcc gcttcgtcga accggccgcg
     8041 ccacgcctgt gtgacggcca gcgtgtgcga cagcgtcgcc tcgtcgacgt ctggatcgcc
     8101 cgcggctgca cggcacgcgt cgacggcgcg ctggtcctgg ccgagccgga gccagccctg
     8161 caccatcacg tgggtcatcg tgaccgaacc gggacccatc tcccggaaca ccggctccat
     8221 ctcgtcgaag cgacgctcgg ccgcgagccg gttcagctcg gtcctgagga tctccacgtg
     8281 gtcgaccttc tgcgcgcgga ggcgctcgac cgcatcgcgc agcgcgcccc ggtcgtcggc
     8341 caacgcgtag acctggagca ggtggaacca cgccgtggcg aaggtcggat cgagctcgag
     8401 cgcccgctcg gacaaaaacc gtacgcggcg cgggtcccag tagcggctcg cgtgcgtcac
     8461 gatttcggac agcacgtagt agagatcggc gatcgggact tgttcgtcgg cgagcgcgtc
     8521 gagcgcgtcg tgggcctcgg cccaccggcc gcgcccgtag tcgacgtagg cgcgatagac
     8581 acgctgccag tcgtccggaa gccgctcgac gtggggcagc ccttgctcga gcgcttcccg
     8641 gctcgcctcg cggtggccgg cccacgagcg cgtcattccc agccgaaagt aggccagggc
     8701 gaaggccggg tcgtgcccga tcgccgcctc gaagtgccgg ccggccgcgt cgaagcgacc
     8761 gaggtggagt tccagctcgc ccgccgcgta ccgccggtag gcttcggccg aggaggtcag
     8821 cgattcggcc agatcgaacg gagccgcccc gtcgcccgag gtcgaaacac cgagctgtcg
     8881 cctgatctcc gcggcgatcg acccggcgag ctcgaacagc tcgctcgctg atccggcctc
     8941 gcgccggatc gagcccgtcg tcttgccggt tcgcacgtcg accagctcgg cggccagcag
     9001 gatccgctcg ccatcccgat tgacctggcc gaccagcatc aactcggcgc ccgcctcgat
     9061 cgccgcgcgg ttcgcgacgg agagatcgaa gccacgatcg accgcgccgc cgacctcgcg
     9121 gatcgccgcc agcacccgcg ccgtcgacag cacgtcgagc ccgcgcgact cgacgaggtc
     9181 ggtcgtcacc aggcccatca gcacccgccc cagcttctcg gcgtcgtcgg ggtcggtgac
     9241 gttctcgaag cccatcaccg cgaccgtgcg accggccgtc agctggtccg gcgctcccgg
     9301 ccacgcaccg ccgctctcgc cgcgcctcag ccacgtccag ccggagacgg ccagcgcgac
     9361 caccacggcg gcggcgatcg cgccgagggc gagccgcgtg ccgttgcgtc gtggccgcgc
     9421 ggcctcgccc gcacccacgg accgcagcgc gtcgcgcagc gcgcccgcgt cctggaagcg
     9481 ccggtccgga tccttctcga ggcaacgcgt gatgaccgcg tcgagcgccg ccggaatcgc
     9541 gtcgcgcagc tcggagaccg gcggcggcgc gtcgcgcagg atcgcggtga tcgtcgagat
     9601 cggcgtctgg cccgcgaacg ggagccggcc ggtcgccatc tcgtagagca tcgtgccgag
     9661 cgagaagacg tcggagcgcg cgtcgacggc gagcccctcc gcctgctcgg gcgacatgta
     9721 gcagaccgtg cccaggatcg cgccgtgcgt cgtcgccgcc gtcaccgttc cgagctgcgt
     9781 cccggcggcg ggcgccgact cgtcggtgag cttggccagg ccgaagtcga gc
//
LOCUS       DASPGN010000267         4341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_397563, whole genome shotgun sequence.
ACCESSION   DASPGN010000267 DASPGN010000000
VERSION     DASPGN010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397563"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1135)
                     /locus_tag="VD788_07835"
     CDS             complement(59..1135)
                     /locus_tag="VD788_07835"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HXV76216.1"
                     /translation="MTAEEIFRGRLVMAPMTRGTDLPFRVLARELGAEVCFGEMAYAH
                     KIVRGARAESVLFRRDPREGLFGVQLAGKHPQLMAEAARIAEDRGADFVDVNLGCPID
                     DATKRGFGASLLRRAGRVAAIVEAMKRAVGIPVTIKLRSGWTADKPTFLKVATAAERA
                     GVDAISLHARSRAQRYRRPADWGLVRALVETVSVPVIGNGDVFDWSDARRRMTETGCA
                     AVMVGRWGLAKPWIFREFHRGRSESPDSAERLAVLRRYVELCREHFGDDEWGRRRTRG
                     FFVFHQDFFRRYRSGGAPGAVNSDDPRDWGDEPDDDIGKWLCRSDRDGVDALSRWLVD
                     GERTEIPPPPGPESPRAVEAPAYG"
     gene            complement(1132..1380)
                     /gene="xseB"
                     /locus_tag="VD788_07840"
     CDS             complement(1132..1380)
                     /gene="xseB"
                     /locus_tag="VD788_07840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXV76217.1"
                     /translation="MAKKQDLGYGRAAERLEEILARIEEGQVDIDELSGLVEEAAGLV
                     TTCRDKIRAAEVRVKSITEQLERDAPQPATAGDGEPDD"
     gene            complement(1383..3170)
                     /gene="xseA"
                     /locus_tag="VD788_07845"
     CDS             complement(1383..3170)
                     /gene="xseA"
                     /locus_tag="VD788_07845"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXV76218.1"
                     /translation="MSRSISHDGQRTLEVRFPFDRDLVELVKTLPGRRWNAVERFWSV
                     PATEVVSLVELLAPAGFGVDRATRELYRGMGGTRELPVEESSAERPTLPGLFDAIDDD
                     AGNPDPTRETTDYTVSGLNERVREAIRLAIPGEVWLVGEISGFDKSAHRRHVSFELVE
                     RADDGTVVSKIPVVLFSQARQAIADRLRLAGDPMRLEDELAVRLRVKVDLYVAWGQYR
                     VVVEDLDVRYTLGEAARRREEIVRRLAAAGLTERNRGLGLPALPLRIGLITSLGSDAF
                     NDVVRTLAESGFAFDVTAHGARVQGGATEASVLNALDYFRARAGSFDVILICRGGGSR
                     TDLVWFDSEALGAAVAEFPLPVLVGIGHEQDLSVLDAVGWSCKTPTAAAALLVERVTR
                     AVERLSELGREVLALANERIGWQRRAVAERAGRLARAARARLRHERELLRGRQRRAVV
                     GSRAAVAAARDRLARWTALIPRDARVLLERQRLWLAGATSTLGRSARHRLERASERIG
                     RTTRAVPPAARRGVERELERTTQRARRLSLVDPRRVLDRGYSILRVEAGRVITRADEA
                     PAGTSLQADLKQGRLRLRSLGGVVADGGD"
     gene            3272..4270
                     /gene="egtD"
                     /locus_tag="VD788_07850"
     CDS             3272..4270
                     /gene="egtD"
                     /locus_tag="VD788_07850"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HXV76219.1"
                     /translation="MPSSRVAIDVHLNESGAMDSIAREVREGLTAPAKRLPSKLFYDE
                     RGSELFERITELPEYYLTRAEQALLVRYAGRIATLTRYEELVELGSGSAKKTRTLLEA
                     GLAAGSLRRFVPVDVSVEISEATARTIAEAYPGLAVHAVVADFEADALKVPPGRRRLV
                     ALLGSTIGNFARREAVALLRRFSALLDGDGRLLVGTDLVKDHAVLEAAYNDTQGVTAE
                     FNLNILNVLNRQLDGDFDPDRFEHVAFFNEDESRIESYLKARTRHIARLEALGLDVPF
                     AAGELLHTEVSCKYTRESVDSMLGDAGMELSHWFTDERATFALSLARRRRRDLAAA"
BASE COUNT          630 a         1628 c         1463 g          620 t
ORIGIN      
        1 gcaccgccat cgattcgcac ctcgctcgtc ccattcggtc gctcgccgcg tgcgtgcctc
       61 agccgtaggc cggggcctcg accgcgcgcg gactctcggg gcccggtgga ggcgggatct
      121 ccgtccgttc cccgtccacc aaccaccgac tcagcgcgtc gacgccgtcc cggtcgctgc
      181 gacacaacca ttttcctata tcgtcgtccg gctcgtcgcc ccagtcgcgc gggtcgtccg
      241 agttgaccgc gcccggcgcg ccgccactgc ggtatcggcg gaagaaatcc tgatggaaga
      301 cgaaaaagcc tcgcgtgcgc cgccgacccc actcgtcgtc gccgaaatgc tcgcggcaca
      361 gctcgacgta gcgccggaga acggccagac gttccgcgct gtcgggcgac tcgctccgcc
      421 cgcggtggaa ctcgcggaag atccagggct tggccagacc ccatcgcccg accatcaccg
      481 ctgcgcaccc ggtctcggtc atccgccgcc gtgcgtcgct ccagtcgaaa acgtcgccgt
      541 tgccgatgac cggcacggag accgtctcga ccagcgcccg caccagtccc cagtcggccg
      601 gccggcggta gcgctgggcg cgactgcgcg catgcagtga aatcgcgtcg acgcccgctc
      661 gctcggccgc cgtcgcgacc ttgaggaagg tcggcttgtc ggcggtccat cccgagcgca
      721 gcttgatcgt gaccgggatg ccaaccgccc gcttcatcgc ctcgacgatc gccgccaccc
      781 gtcccgcccg acggagcagc gacgcgccga agccgcgttt ggtcgcgtcg tcgatcgggc
      841 aacccaggtt cacgtcgacg aaatccgccc cgcggtcctc cgcgatgcgg gccgcttccg
      901 ccatcagctg cgggtgcttg ccggccaatt ggacgccgaa cagcccttcg cgcgggtcgc
      961 gcctgaagag cacactctcg gctcgcgcgc cacgaacgat cttgtgcgca tacgccatct
     1021 cgccgaagca gacctcggca cccagctctc gcgccagtac acggaagggg agatcggtcc
     1081 cccgtgtcat cggcgccatc accaggcgac cgcggaagat ctcctcggcc gtcaatcgtc
     1141 cggctcgccg tcgccggccg tggcaggctg cggcgcatcc cgctcgagct gctcggtgat
     1201 gctcttgacc ctcacctcgg ccgcccggat cttgtcgcga caagtggtca ccaggccggc
     1261 ggcctcctcg acgagtcccg acagctcgtc gatgtcgacc tgcccttcct cgatgcgggc
     1321 caggatctcc tcgagccgct ccgccgcccg accgtagccc aggtcttgct tcttcgccat
     1381 gctcaatcgc ctccgtcggc cacgacgccc ccgagcgagc gcagccgcaa tcgcccctgc
     1441 ttcaagtcgg cctgcagcga ggtgccggcc ggcgcctcgt cggcgcgcgt gatcacgcgg
     1501 cccgcctcga cgcgcaggat cgagtacccg cggtcgagca cgcgtcgcgg atcgaccagc
     1561 gagagccgcc gggcacgctg ggtcgtccgc tcgagctccc gctcgacccc gcggcgggcg
     1621 gcggggggga cggcgcgcgt cgtgcggccg atccgctcgc tcgcgcgctc cagccgatgc
     1681 cgcgccgacc gacccagcgt cgacgtcgcg ccggcgagcc acagccgctg tcgctcgagc
     1741 agcacgcgtg cgtcgcgcgg gatcagcgcg gtccagcgcg ccagccggtc gcgcgccgcg
     1801 gcgacggctg cgcgcgagcc gacgacggcg cggcgctggc ggcccctcag cagctcacgc
     1861 tcgtggcgca gccgggcacg ggcggcgcgg gcgagccgtc ccgcccgctc ggccaccgcg
     1921 cgccgttgcc atccgatccg ttcgttggcc agcgccagca cctcccgtcc gagctcggac
     1981 aagcgctcga ccgcacgggt cacccgttcg acgaggagcg cggccgccgc ggtcggcgtc
     2041 ttgcagctcc acccgacggc gtcgagcacc gacagatcct gctcgtgacc gatcccgacc
     2101 aacaccggca gcgggaattc ggcgacggcc gcgcccagtg cctcggagtc gaaccaaacc
     2161 agatcggtgc gggagccgcc gccgcggcag atcaggatca cgtcgaacga gccggcgcgc
     2221 gcgcggaagt aatcgagcgc gttgagcacc gacgcctcgg tggcaccgcc ctggacccgg
     2281 gccccatgcg cggtcacgtc gaacgcgaat cccgactccg cgagcgtgcg gaccacgtcg
     2341 ttgaaggcgt ccgagccgag actcgtgatc agaccgatcc gcaacggcag cgccggcaac
     2401 ccgagccccc ggttgcgctc ggtcaacccg gcggcggcga gccgccgcac gatctcctcc
     2461 cgtcggcggg cggcctcgcc cagcgtgtag cgcacgtcga gatcctcgac gacgacgcgg
     2521 tactgacccc atgcgacgta gaggtcgacc ttgacgcgaa gacgaacggc gagctcgtcc
     2581 tcgaggcgca tcgggtcgcc cgccaggcgc agccgatcgg cgatcgcctg gcgcgcctgc
     2641 gagaagagca cgaccgggat cttcgagacg acggtgccgt cgtcggcccg ttcgacgagc
     2701 tcgaacgaga cgtggcgacg gtgcgcgctc ttgtcgaacc cgctgatctc gccgacgagc
     2761 cagacttcgc cggggatagc gaggcgaatc gcctcgcgga cgcgctcgtt cagccccgag
     2821 accgtgtagt cggtcgtctc ccgggtcggg tcggggttcc cggcgtcgtc gtcgatcgcg
     2881 tcgaacaggc ccgggagcgt cggccgctcg gccgacgact cctcgaccgg cagctcgcgg
     2941 gtgcccccca tgccgcggta caactcgcgc gtcgcgcggt cgacgccgaa gccggcggga
     3001 gccagcagct cgaccagcga cacgacttcg gtcgcgggca ccgaccagaa tcgctccacc
     3061 gcgttccacc tccggccggg cagcgtcttg accaattcga ccaggtcgcg gtcgaacgga
     3121 aagcggacct cgagcgtgcg ctggccgtcg tggctgatgc tgcgactcat cggctcgaag
     3181 gctagcgcac cgaacagccc cgtctcaagc gtcgtgcctc ggcgggggtg cgagtttgga
     3241 atttcgatcg cgagcggata gtatctgggc catgccgtcg tcgcgagtcg ccatcgacgt
     3301 ccacctgaac gagtcgggcg cgatggactc gatcgcccgc gaggtgcgcg agggcttgac
     3361 cgcgcccgcc aagcggcttc cgtcgaagct gttctacgac gagcgcggca gcgagctgtt
     3421 cgagcggatc accgagctgc ccgagtacta cctgacgcgc gccgagcagg cgctgctcgt
     3481 gcggtacgcc ggccgcatcg cgacgctgac gcgctacgag gagctggtcg agctcggttc
     3541 gggctcggcg aagaagacgc gcacgctgct cgaggccggt ctggcggcgg gatcgctccg
     3601 ccgcttcgtg cccgtcgacg tctcggtcga gatctccgag gcgacggcgc ggacgatcgc
     3661 cgaggcctac cccggactcg ccgtccacgc ggtcgtcgcc gatttcgagg ccgacgcgct
     3721 caaggttccg ccggggcggc ggcggctggt cgcgctgctc gggagcacga tcggcaactt
     3781 cgcgcggcgc gaggccgtcg cgctgctgcg acggttctcg gcactgctcg acggcgacgg
     3841 tcgattgctg gtcggcaccg atctcgtcaa ggaccacgca gtgctcgaag cggcctacaa
     3901 cgacacgcag ggcgtgaccg cggagttcaa cctgaacatc ctcaacgtgc tcaatcgcca
     3961 gctggacggc gacttcgacc ccgaccgctt cgagcacgtc gcgttcttca acgaggacga
     4021 atcgcggatc gagtcctatc tcaaggcacg cacccggcac atcgctcgtc tcgaggcact
     4081 cggcctcgac gtgccgttcg cggccggcga gctgctccac accgaggtgt cgtgcaagta
     4141 cacgcgtgag tcggtcgact cgatgttggg cgatgccggc atggagctgt cccactggtt
     4201 caccgacgag cgggccacgt tcgccctgtc gctggcgcgg cggcggcgcc gggacctggc
     4261 ggccgcctag gagcctgtcc gagtattgcc tacggccgcg ttccggacgc cgcggggccg
     4321 gatgcaaggc gccgcgctgc a
//
LOCUS       DASPGN010000268         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1686310, whole genome shotgun sequence.
ACCESSION   DASPGN010000268 DASPGN010000000
VERSION     DASPGN010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686310"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VD788_07855"
     CDS             complement(<1..327)
                     /locus_tag="VD788_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HXV76220.1"
                     /translation="MKSGFTAKSKRILRLNVNGGVWIKPGAAIAYTGAFRFERQATTD
                     ARSAQDALFRELLPLVRAVGQGRLFCADYGSHVRIVQIDKEPLVVAGPHVLAFEEAVA
                     FDRSLLG"
     gene            complement(324..1280)
                     /locus_tag="VD788_07860"
     CDS             complement(324..1280)
                     /locus_tag="VD788_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXV76221.1"
                     /translation="MVLAMAMVASPAYAQLNGENLLGDMGAKSGTQPAPGLYLGNVFY
                     RYKTDTIKGPDGKTIAPDPTGDARQTINAAVPMTIYITKKKVLGANFGMMAVMPFANG
                     SLEAPGFGLSEKASTGPSDLYVMPAQLGWHKRRFDATTGFAFFAPTGRHTAGASDNLG
                     KGMWSYELSAGSTLYLDERRSISLAATAYWETHSKKVGEVNVQGMTIGDVKVGQLLTI
                     EGGAAKSFLHGAAHVGIAYYGQWKVTEDQFGLSSIELPVSDKHRVFGIGPDVTIPIAS
                     KKKLLSLVNVRYMFEVGTRTKTQGQSLVITSTVPVGGIKISR"
     gene            complement(1318..>2292)
                     /locus_tag="VD788_07865"
     CDS             complement(1318..>2292)
                     /locus_tag="VD788_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXV76222.1"
                     /translation="YTSDAVEFLRKYDGDKPFCLIVTLVNPHDVQEYPGRGVRGISKD
                     PTFVKGGYRLADFRDLPIDLPPNFADDLSTKPSVQASFRKILGVGTGHVGTRERQLTY
                     ARFYAYLNQQVDAQIGRVLDALDARGLTNDTVIVRTSDHGELAMSHGRMRQKFYNVYR
                     ETLNVPLIVSNPLLYPKAQSTDALASLIDLVPTLATIGGVPEPEQYGLKGVDLTPVLE
                     DPKALVQDVLHFTYEDDVFPVDGADCIRAIVERDWKYAVYYDPFTGAPTEYEMYDLAR
                     DPLELSNLANATHATQATEAERARLHRRLTDVMRGHGTTPDEINWPIF"
BASE COUNT          391 a          725 c          725 g          451 t
ORIGIN      
        1 gccgagcagg ctcctgtcga atgccaccgc ctcttcgaac gccaggacgt gaggcccggc
       61 gacgacgaga ggctccttgt cgatctgaac gatccgcacg tgcgaaccgt agtcggcgca
      121 gaacagccgc ccttgcccca ccgccctgac cagcggcagg agctcgcgga acagggcatc
      181 ctgagctgac cgggcatccg tggtcgcctg ccgctcgaac ctgaacgcgc ctgtgtaggc
      241 gatcgcggcg cctggtttga tccagacgcc gccattgacg ttcaggcgca ggatgcgctt
      301 cgactttgcc gtgaacccgc tcttcatcgc gagatcttga tcccgccaac cgggacggtt
      361 gatgtaatga ccagcgactg accctgtgtc ttggtccgcg tgccgacttc gaacatgtag
      421 cggacgttca cgagcgacag cagcttcttc ttcgacgcga tcgggattgt cacgtcgggg
      481 ccgatgccga acacccggtg cttgtcggac accggcagct caatggacga gaggccgaac
      541 tgatcttcgg tcaccttcca ctgtccgtag tacgcgatcc cgacgtgcgc agcgccgtgc
      601 aggaacgact tggcggcacc gccttcgatg gtgaggagct gcccgacttt gacgtcgccg
      661 atcgtcatcc cctgcacatt gacctcgccg accttcttcg aatgcgtctc ccagtaggcg
      721 gtcgccgcga ggctgatcga tcgccgctca tcgagataca gcgtcgaacc ggccgacagt
      781 tcatagctcc acatgccctt gcccaggttg tcgctcgcgc cggcagtgtg gcgtccggtg
      841 ggagcgaaga acgcaaagcc ggtggtcgcg tcgaaccgcc gcttgtgcca gccgagttgc
      901 gccggcatca cgtaaaggtc gcttggaccg gtgctcgcct tctcggaaag accgaagccg
      961 ggagcttcga gcgaaccgtt cgcgaacggc atgaccgcca tcatcccgaa gttggcgcca
     1021 agcaccttct tcttcgttat gtagatcgtc atcggcaccg cggcgttgat cgtctgtctc
     1081 gcgtcgccgg tcggatcggg cgcgatggtc ttgccgtcag gccccttgat cgtgtcggtc
     1141 ttgtaacgat agaacacgtt gccgaggtag aggcccggag cgggctgggt gccgctcttc
     1201 gctcccatgt cgccgagcag gttctcgccg ttgagctgcg cgtatgccgg cgaggcgacc
     1261 atcgccatcg cgagcaccat cttgaacaca ctcttcccac tcatgtcgtc tctccttcta
     1321 gaaaatcggc cagttgattt cgtccggggt ggtaccgtgc ccgcgcatca cgtcggtcag
     1381 ccggcggtga aggcgggcgc gttccgcctc cgttgcctgc gttgcgtgcg tcgcgttcgc
     1441 caggttcgac agttcgagcg gatcgcgcgc gaggtcgtac atctcgtact cggtcggcgc
     1501 gcccgtgaag gggtcgtagt aaacggcgta cttccagtca cgctcgacga tggcgcggat
     1561 acaatcggcc ccgtcgaccg ggaagacatc gtcttcgtag gtgaagtgca ggacgtcttg
     1621 tactaaagcc tttgggtcct cgaggactgg cgtcaggtcg acgcccttca gcccgtactg
     1681 ctcgggttcg ggaacgccgc cgatggtcgc gagggttggc acaaggtcga tcaggctcgc
     1741 cagcgcgtcg gtggactgtg ctttcggata cagcagcgga ttcgagacga tcagcgggac
     1801 gttcagcgtc tcgcgataga cgttgtagaa cttctgtcgc atcctgccgt gcgacattgc
     1861 gagttcgccg tgatcgctgg tgcggacgat gaccgtgtcg ttggtcagac cgcgggcgtc
     1921 gagggcgtcc agcacgcgcc cgatctgcgc atccacctgc tgattgaggt aggcatagaa
     1981 cctggcgtag gtcagctgcc gctcgcgggt accgacgtga ccggtgccaa cgccgagaat
     2041 tttccggaag gacgcctgaa cgctcggctt cgtcgacagg tcgtcggcga agttcggggg
     2101 cagatcgatc ggcagatccc tgaagtcggc aaggcggtag ccgcccttca cgaacgtcgg
     2161 atccttcgag atcccacgca cgccgcgacc cgggtactcc tgaacgtcgt gcggattgac
     2221 cagtgtgacg atcaagcaga agggcttgtc gccgtcatac ttccgcagaa actcgaccgc
     2281 gtccgacgta ta
//
LOCUS       DASPGN010000269         4297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_983399, whole genome shotgun sequence.
ACCESSION   DASPGN010000269 DASPGN010000000
VERSION     DASPGN010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983399"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1200
                     /locus_tag="VD788_07870"
     CDS             19..1200
                     /locus_tag="VD788_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV76223.1"
                     /translation="MIRRRARPAAIALLLLVTSQLAVPAQDSERRRGFDVTILEPVMQ
                     EVVFGKTKIAAGVEIDDPDLVDRVEFLIGEEIVFVDREAPYEFFYDFGEEQRSWVVRV
                     VAWHKEGVSVSDVTITRKLSFSNIESVNRVILWVSAQDRDGNFVTDLNRGDFQIYEDG
                     AEQKIIDFYAENRAITLAIVIDTSGSMRDKIREVHEAAGAFVDTVREIDQAMVIDFDD
                     SVFLIQDLTNDRDALREAITSTEPIGATSLYDAVHATYRKIGKVDGRKATILLSDGED
                     TSSQFGYKRVLQEAKSNNMLLYTIGIEGGGIDKSVLREFADNTGGRAYFVTKASELGQ
                     VYQRIAEELGKQYYLTYSTSNENWDGHWIKIDVESRRPGVTIRARKGYFAVRGVDRAG
                     G"
     gene            complement(1203..1982)
                     /locus_tag="VD788_07875"
     CDS             complement(1203..1982)
                     /locus_tag="VD788_07875"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXV76224.1"
                     /translation="MSRFLPALLLCLVGAAAADRPAPDDPPAPAPASGETALARVSRL
                     QGSAFLDRNRDVVGAAVIVHRMGDVSKVYLTSTDEFGRFKIEGLPDGEYRVRVERHGV
                     APVAKNDVSVKFPFRAVVELDMEALERGPIVPRTTGYTPQTSGTVTVRGTIRGTGGQP
                     MGEAHVRLVRTDAAFDPQTATTDADGEFSLAEVPVGEWVATVYGVGHLPIRTTVVVDH
                     DAEVEIGLVRQPASYKPSPLELMPVEEPIRPPGLEQAKSKI"
     gene            2300..2375
                     /locus_tag="VD788_07880"
     tRNA            2300..2375
                     /locus_tag="VD788_07880"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2333..2335,aa:Val,seq:tac)
     gene            complement(2379..3101)
                     /locus_tag="VD788_07885"
     CDS             complement(2379..3101)
                     /locus_tag="VD788_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXV76225.1"
                     /translation="MVLPPGNLILLSLAGFLLFRRWPRVGLSLLAASSIGLVALSAPV
                     CSSLLLRSLETYPPLTEGDYPADVQAIVILGAESYSPAREFGTDTIGGTTLERVRYGA
                     RLHRATGVPILTTGGTIRPANVPVGRAMAEVLAGEFGVPVRWTETESSNTFENAKFSA
                     ALLERDGVGKIYLVTTASHIPRAKLAFEAMGLEVVPAPTSLTRRPTPILGDFLPRIKA
                     LHDSHDAFYEWLGLWWYRLAYF"
     gene            3301..3377
                     /locus_tag="VD788_07890"
     tRNA            3301..3377
                     /locus_tag="VD788_07890"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3335..3337,aa:Asp,seq:gtc)
     gene            complement(3412..4011)
                     /locus_tag="VD788_07895"
     CDS             complement(3412..4011)
                     /locus_tag="VD788_07895"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HXV76226.1"
                     /translation="MSRRSSFIVLMLAVLAGPPALAQKITIDYDKDFRGKVETFAWYE
                     HDNEATSLAVQNPLVHSRIVNAIEHFLTTIGLREVGPDATPDLLVTYHTSSTQEVEYN
                     TTNFGYGYPGGWYSPYGYGWGGGASTTTATTYQRGTLVLDAWDAKTEKLIWRGTASAI
                     IPENPEKVTKVIDDALDKMVRKWDKIRKQLRAEAEKGGP"
BASE COUNT          714 a         1416 c         1457 g          710 t
ORIGIN      
        1 cggtccggca ggtgactcgt gatccgacgc cgtgcccggc cggcggcgat cgccctactg
       61 ctgctggtca cgtcgcagct cgccgtcccg gcgcaagact ccgagcgacg gcgcgggttc
      121 gacgtgacaa tcctcgagcc ggtgatgcag gaggtcgtgt tcggcaagac gaagatcgcc
      181 gccggagtcg agatcgacga ccccgatctg gtcgatcgcg tcgagttcct gatcggcgaa
      241 gagatcgtgt tcgtcgaccg cgaggcgccc tacgagttct tttacgactt cggcgaagag
      301 cagcgctcgt gggtcgtccg cgtggtggcc tggcacaagg aaggcgtatc ggtcagcgac
      361 gtgacgatca cccgcaagct gtcgttctcg aacatcgagt cggtcaatcg ggtgattctc
      421 tgggtttcgg cgcaggaccg cgacggcaac ttcgtcaccg atctgaatcg aggcgacttc
      481 cagatctacg aggacggcgc ggagcagaag atcatcgatt tctacgccga gaaccgcgcg
      541 atcacgttgg cgatcgtcat cgacacttcg ggctcgatgc gagacaagat ccgcgaggtg
      601 cacgaggccg ccggcgcctt cgtcgacacc gtgcgcgaga tcgatcaggc gatggtgatc
      661 gacttcgacg acagcgtctt cctgatccag gatctgacca acgaccgcga cgcgttgcgc
      721 gaggcgatca ccagcaccga gccgatcggt gccacttcac tctacgacgc ggtccatgcc
      781 acctaccgca agatcggcaa ggtcgacggt cgcaaggcga cgatcctgct gtcggacggc
      841 gaggatacgt cgagccagtt cggctacaag cgcgtgttgc aggaggcgaa atcgaacaac
      901 atgctgctgt acacgatcgg catcgagggc ggcggcatcg acaagagcgt gttgcgcgag
      961 tttgccgaca acaccggcgg ccgcgcctac ttcgtcacca aggccagcga gctcggccag
     1021 gtttatcagc ggatcgccga agagctgggc aagcagtact acctgaccta ctcgacgagc
     1081 aacgagaact gggacgggca ctggatcaag atcgacgtcg aatcgcggcg accgggtgtg
     1141 acgattcgtg cgcgcaaggg ctatttcgcg gtgcgcggcg tcgatcgcgc cggcggctga
     1201 cctcagatct tcgacttggc ctgctccagc ccgggcgggc ggatcggttc ttcgaccggc
     1261 atcaactcga gcggggacgg cttgtacgac gccggctggc gcacgaggcc gatctcgacc
     1321 tcggcatcgt ggtcgaccac gaccgtggta cggatcggca ggtggccgac gccgtagacc
     1381 gtggccaccc actccccgac cggcacctcc gccagcgaga attcaccgtc ggcgtcggtc
     1441 gtcgcggtct gcgggtcgaa cgccgcgtcg gtgcggacga gccggacgtg cgcttcgccc
     1501 atcggctggc cacccgtgcc gcggatcgtg ccgcgcaccg tcaccgtgcc cgaggtctgc
     1561 ggcgtgtagc cggtcgtgcg cggaacgatc ggcccgcgct cgagcgcttc catgtcgagc
     1621 tcgaccaccg cgcggaacgg gaacttcacg ctgacgtcgt tcttggctac gggcgcgacc
     1681 ccgtgccgct cgacgcgcac gcggtactcg ccgtcgggta gaccctcgat cttgaaccgt
     1741 ccgaactcgt cggtcgacgt caggtagacc ttggacacgt cacccatccg gtggacgatg
     1801 accgcggcgc cgacgacgtc gcggttgcga tcgagaaagg cgctcccctg cagccggctg
     1861 acccgggcca gcgccgtctc gccgctcgcc ggcgccggcg ccggcggatc gtccggcgcg
     1921 ggccggtccg ccgccgccgc accgacgagg cacaacagca acgccggtag gaaccgcgac
     1981 attggtagaa atcaggcgcg gtgcccttgc gggcccgggt tcctgaatcc ccctttcaaa
     2041 ccgtgcgtgc gaatttcccg cacacggctt acgaatcgtc ttctcatgct gcgtgcacgc
     2101 ctccgaaccg aagcctgcta cgcggcgccc cggcaggcct accgcggcgg gacttgcacc
     2161 cgcaagtccg gtacagcatc tcggctcccc cgcgggagct tggttcagga cgcgacatgg
     2221 gctgcattag agaggctcgc cggcggccga ccagcgcggc ggagcgggtg ctatgatcgg
     2281 ccggcccgcc agcgagatcg ggcggttagc tcagctggaa gagcgcctgc cttacacgca
     2341 gggtgtcggg ggttcaagtc cctcaccgcc caccacgatc agaaataggc caagcggtac
     2401 caccacaggc cgagccattc gtagaacgca tcgtggctgt cgtgcagcgc cttgatccgc
     2461 ggcaggaagt cgccgaggat cggtgtcggc cgacgcgtca gactcgtcgg tgcggggacg
     2521 acctccaacc ccatcgcctc gaacgcgagc ttggcgcggg ggatgtgcga tgcggtggtg
     2581 acgaggtaga tcttgccgac gccatcgcgc tcgagcagtg ccgcgctgaa cttggcgttc
     2641 tcgaaggtgt tggacgactc cgtctcggtc caacgcaccg gcacgccgaa ctcgccggcg
     2701 agcacctcgg ccatcgcccg gccgaccggg acgttggccg ggcggatcgt cccgccggtg
     2761 gtgaggatcg gcacgcccgt ggcgcgatgc aatcgcgcgc cgtagcggac gcgttcgagc
     2821 gtcgtgccgc cgatcgtgtc ggtgccgaac tcccgagccg gcgaatacga ttcagcgccg
     2881 agtatcacga tcgcctgcac gtccgccgga tagtcgcctt cggtcagcgg cgggtaggtc
     2941 tcgagcgagc gcagcagcag gctgctgcag accggcgcgc tgagcgcgac caggccgatc
     3001 gacgaggcgg cgagcaacga caggccgacg cgcggccagc gccggaacaa caggaacccg
     3061 gcgagcgata gcaggatcaa gttgccgggc ggcagaacca aaatctttat caagacgacg
     3121 gtgacctgga accaacgtgt aggtgaaggg tgcggcggcg gtccccccgg gcgagccccg
     3181 gtgacgggcg gcgccgcgtg ggatcatagc gggctcgacg gccgttgacg aaaccgaggc
     3241 gattgattac agtgacgggg tttcggggcc gcccgaggcc tcggcgacgg cttgcggcac
     3301 ggagccgtgg tgtagctggt taacataccg gactgtcact ccggagatcg cgggttcgag
     3361 tcccgtcggc tccgccatca ggccgctcgc acatgagggt ccgccgcttc gttacgggcc
     3421 gcctttctcc gcttcggccc gcagctgctt gcgaatcttg tcccactttc ggaccatctt
     3481 gtcgagtgcg tcgtcgatga ccttcgtcac cttctcgggg ttctcgggga tgatcgccga
     3541 ggccgtgccg cgccagatca gcttttcggt cttggcgtcc cacgcatcga ggaccagcgt
     3601 cccgcgctgg taggtcgtcg cggtcgttgt cgaggcccca ccgccccagc cgtagccgta
     3661 cggcgaatac cagccgcccg ggtagccgta gccgaagttg gtcgtgttgt actccacctc
     3721 ttgcgtgctg ctggtgtggt acgtcacgag gaggtccggg gtggcgtccg gtccgacctc
     3781 gcgcaggccg atcgtcgtca gaaagtgctc gatcgcattg acgatgcggg aatggaccag
     3841 cggattctgg acggcgagcg acgtcgcctc gttatcgtgc tcgtaccagg cgaaggtctc
     3901 gaccttcccg cggaagtcct tgtcgtagtc gatggtgatc ttctgggcca aggcaggcgg
     3961 accggctagg accgccagca tcagaacaat gaacgagctt cgacggctca tcggctctcc
     4021 tcctcgcttc tcgggccgat cgacggtgtc cgagcaccgc ggggcatccc gcggcccgtc
     4081 cagggtagac gctcggtggc tcgggcgcaa agaccggatt cgtcgtacta atgaaggccg
     4141 cccctccgct tgcggcccgc cgcggcggag ccgagattgg gatcgcaccc tgacgggccg
     4201 aggagtcgct gaccttgccg gtatcgagaa aacgcgcacc gtggctcgtc ctgctgccgc
     4261 tcgccgtcgt gtcgctccac gcgggcgatg ccgaggt
//
LOCUS       DASPGN010000270         4010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1686654, whole genome shotgun sequence.
ACCESSION   DASPGN010000270 DASPGN010000000
VERSION     DASPGN010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1686654"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VD788_07900"
     CDS             <1..197
                     /locus_tag="VD788_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76227.1"
                     /translation="RAAEAGGRDRLRPARPLVQRIVLAYDRREEARRPSAESPGAPLA
                     VEPGDQTQRLRVAHDGEDGS"
     gene            194..2542
                     /locus_tag="VD788_07905"
     CDS             194..2542
                     /locus_tag="VD788_07905"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HXV76228.1"
                     /translation="MNDEVPARGAKSGRSTQRTRRLSLVSRVRATVRRFADRLLGTDV
                     LWSGLFVAVTLSIVAAQRCGTGYEQFAVGEVAPYDIRATYDIDVVDEALTDERRHQAR
                     VQILDVYVHDSERRSLLANQLTDVFEDGRSAIEIAAQRGEPRSGALERALGDRVPVDV
                     LRTLSRTEFDAGLERTLTSAVSLVMSRMIIGNKALLEREPAIMLTQLPGRATRRMDDY
                     DATIDTRAARDELQQLLRREMKLGADEQSTIIRFAQSYIDANVYLDSEATYRRREQAA
                     AAVPPVLLRVASGDLMIRKGERITEDALAKLDAARKQSPGPLDLVEALGLMLMLCMLA
                     FFIYRYSSYHQRNFRKITHLHALLVLMLLSLCLLTQAILWLAGEIAEDLSPPFGDLEA
                     YHYLIPLGAGAILVALLANGRIATVYSAFAAVVFGGIRGADFYLMLWAFVVQLSGVYA
                     IVTYRERAALLRAGLVVGGMGAVSAVAIEILRGSVEPISSVMFAASLAFVGGAVGVGL
                     LISFSLPLLEWLFNVLTDIRLLELSNVNTPLLSEMAVKAPGSYNHSLVVGTLAEEGAK
                     AIGANSLLCRVAAFYHDIGKMNHPEYYVENQRGVNPHDRLAPSMSALIITSHVKDGIK
                     MAREAGLPEQIVDMIPQHHGTKKLTFFYEKAKQRSDPSLGPVNEEDFRYPGPKPQTRE
                     GAIFMIADGVEAAARTVEDPTPNRLKEMIRKVTNSIVLDGQFDQCDLTFADLERIQQA
                     LLRLLVSMHHRRVDYPGFEFGRPRAVAKAPGDGSGRKVVRGA"
     gene            2544..2996
                     /gene="ybeY"
                     /locus_tag="VD788_07910"
     CDS             2544..2996
                     /gene="ybeY"
                     /locus_tag="VD788_07910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXV76229.1"
                     /translation="MSVSVVNRQRSRRVPRQGLSTFLRRADRLVAAGRGGELTVCLVS
                     DRKMRELSRVYRGRDAPTDVLSFPAGGDESEPGRRYLGDIAVSVETAARQARQRGHAL
                     AVELKILVLHGYLHLLGLDHETDDGQMMRLQRRLERRLLDPGWPGKRR"
     gene            2993..>4010
                     /locus_tag="VD788_07915"
     CDS             2993..>4010
                     /locus_tag="VD788_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXV76230.1"
                     /translation="MIEPATRLIDVWPFAVAAVLLVIDLSLVIALSAMTALGPVSLRR
                     FSSERMPHLAFVENLPRPDSAHKMAAHGLQQICLLGTMTLVAYALRLRGVATPALVAW
                     LGTVLVMVLGLHMFVARGLAMWKPHRAFPVTLWSMRLAFVCLYPLVVAARAWIRRIAE
                     VQARLEDERDEDQDEQVEALIEVGERSGLLEAEEGKMMRGIVDLDETLVREIMTPRTE
                     IVAVEVGTTVDEARGRILDSGHSRLPVYRETIDNVVGVLHSRDLFQAWKAGEDDRAVA
                     HYLRPAVFVPETLSAAELLAEMRQRTQIALVVDEYGGVAGLITLEDVLEEIVGDIRDE
                     HDSEE"
BASE COUNT          635 a         1307 c         1378 g          690 t
ORIGIN      
        1 gccgagcagc agaagctggc ggtcgagacc ggctacgtcc cgctcgtccg ctggtccaac
       61 gcatcgtgct ggcctacgac cgccgcgagg aggcacgccg gccgagcgcg gagagcccgg
      121 gcgcgccgct cgccgtcgag ccgggtgacc agacgcaacg gctccgcgtc gcgcacgatg
      181 gggaggacgg gtcgtgaacg acgaggtccc cgcgcgtggg gcgaaaagcg gtcgctcgac
      241 ccagcgcacc cgcaggctct cgctcgtctc ccgtgtccgc gccacggtac gtcgattcgc
      301 cgatcgcttg ctcggcaccg acgtgttgtg gtcgggactg ttcgtcgcgg tgaccttgtc
      361 gatcgtcgcc gcgcaacgct gtggaacggg atacgagcag ttcgcggtcg gcgaggtcgc
      421 cccctacgac atccgtgcga cctacgacat cgacgtggtg gacgaagcgc tcaccgacga
      481 acggcgccac caggctcgcg tccagatcct ggacgtctac gtccacgatt ccgagcgccg
      541 tagcctgttg gccaatcagc tgaccgacgt gttcgaagac gggcgcagcg cgatcgagat
      601 cgcagcgcaa cggggggagc cgcgatcggg ggcactcgag cgggcgctgg gcgatcgcgt
      661 cccggtcgac gtgctgcgca cgttgtcccg caccgagttc gacgccggcc tcgagcgcac
      721 gctgacctcc gcggtctcgc tcgtcatgag ccggatgatc atcggcaaca aggcactgct
      781 cgaacgcgag ccggcgatca tgctgacgca actgccgggc cgtgccacgc gtcgcatgga
      841 cgactacgac gcgacgatcg acacgcgcgc cgctcgtgac gaacttcagc agcttctgcg
      901 acgagaaatg aagctcggcg cggacgagca atcgacgatc atccgtttcg cgcagtccta
      961 catcgacgcc aacgtctacc tcgactcgga ggcgacctat cgccggcgtg agcaggcggc
     1021 cgcggcggtg cccccggtgc tgctgcgtgt cgccagcggc gacctgatga tccgcaaggg
     1081 cgagcgcatc accgaggacg cgttggccaa gctggacgcc gcgcgcaagc agtcgccggg
     1141 gccactcgac ttggtcgagg cgctcggcct gatgctgatg ctgtgcatgc tggcgttttt
     1201 catctaccgc tacagcagct accaccaacg caactttcgc aagatcaccc acctgcacgc
     1261 gctgttggtg ctgatgctgc tgagcctctg cctgctgact caggcgatcc tgtggcttgc
     1321 cggcgaaatc gccgaggacc tgtcgccgcc gttcggtgat ctggaagcct accattacct
     1381 gatcccgctg ggtgccggcg cgatcctcgt cgcgttgctg gccaacgggc gtatcgcgac
     1441 ggtctactcg gcgttcgcgg cggtcgtctt cggcgggatc cggggggccg atttctacct
     1501 gatgctgtgg gcgttcgtcg tccagctcag cggtgtctat gcgatcgtca cctatcgcga
     1561 acgggccgcc ctgctgcgcg ccgggctggt ggtcggcgga atgggcgcgg tctccgccgt
     1621 tgccatcgag attctgcgtg gcagcgtcga gccgatttct tcggtgatgt tcgccgcgtc
     1681 gctggcgttc gtcggtggcg cggtcggtgt cggtctgctg atctcgttca gtctgccgct
     1741 gctcgagtgg ctgttcaacg tcctgaccga tatccgactg ctcgagctgt ccaacgtcaa
     1801 cactccgttg ttgtcggaga tggcggtcaa ggcgcccggc tcctacaacc actcgctggt
     1861 ggtcggcacg ctggccgaag agggtgcaaa ggcgatcggg gccaattcgc tgctctgccg
     1921 cgtcgcggct ttctaccacg acatcggcaa gatgaaccac ccggagtact acgtcgagaa
     1981 ccagcgtggg gtcaaccccc acgaccggct cgcaccgtcg atgtcggcgc tgatcatcac
     2041 ctcgcacgta aaggacggga tcaagatggc acgcgaggcc ggcctgcccg agcagatcgt
     2101 cgacatgatc ccgcagcatc acggaacgaa gaaactgacc ttcttttacg agaaggccaa
     2161 gcaacggtcg gatccttccc tcggcccggt caacgaggag gattttcgct acccgggacc
     2221 caagccgcag acccgcgagg gggcgatctt catgatcgcc gacggggtcg aggcggccgc
     2281 cagaaccgtc gaggacccga cccccaatcg cttgaaagaa atgatccgca aggtcaccaa
     2341 ctcgatcgtg ctcgacgggc agttcgatca gtgcgacctg acgttcgccg atctggagcg
     2401 gatccagcag gccctgttgc gccttctcgt cagcatgcac catcgacgcg tcgactatcc
     2461 ggggttcgag ttcggccgcc cgcgagcggt ggccaaggcg ccgggggacg ggtcgggtcg
     2521 gaaggtcgtg cgtggcgcct gagttgagcg taagcgtggt caaccgacag cgaagccggc
     2581 gggtgccgcg gcaggggttg tccacgttcc tgcggcgggc ggaccgattg gtcgccgccg
     2641 gccggggggg cgagctgacc gtttgtctgg tgtcggatcg caagatgcgg gagctctctc
     2701 gcgtctatcg aggccgggat gcgccgaccg acgtgctgtc gtttccggcg ggcggcgacg
     2761 agtcggaacc cggccggcgt tatctcggcg acatcgcggt ctcggtcgaa acggccgccc
     2821 ggcaggcgcg ccagcggggc cacgcattgg ccgtcgagtt gaagatcctc gtgcttcacg
     2881 ggtacctgca cctgttgggg ctcgaccacg aaaccgacga cggccagatg atgcggctgc
     2941 agcgacggct cgagcgccga ctgctcgacc ccggctggcc ggggaaacgg cgttgatcga
     3001 acccgcgacc aggctgatcg acgtctggcc gttcgccgtc gccgccgtgc tgctggtcat
     3061 cgacctgtcg ctggtgatcg cgctcagtgc gatgaccgct ctcggaccgg tctcgctgcg
     3121 ccgcttcagc tccgagcgaa tgcctcacct ggccttcgtc gagaacctgc cacgccccga
     3181 ctccgctcac aagatggccg cgcacgggct gcagcagatc tgcttgctcg gcacgatgac
     3241 gctggttgcg tacgccttgc gtctgcgcgg cgtcgcgacg ccggcgctgg tggcctggct
     3301 cgggaccgta ctcgtgatgg tcctcggttt gcacatgttc gtcgctcgcg ggctcgcgat
     3361 gtggaagccc caccgggcgt tccccgtgac gttgtggtcg atgaggctgg ccttcgtttg
     3421 tttgtacccg ttggtcgtcg ccgcacgcgc ctggatccgc cgcatcgcag aggtccaggc
     3481 gcggttggag gacgagcgcg acgaggacca ggacgaacag gtcgaggcat tgatcgaggt
     3541 cggggaacgc agcggcctgc tcgaagccga agagggcaag atgatgcgcg gcatcgtcga
     3601 tctggacgag acgctggtgc gtgagatcat gacgccgcgt accgaaatcg tcgcggtcga
     3661 ggtcggcacg acggtcgacg aagcccgcgg ccgaatcctc gacagcggcc attcccgcct
     3721 gccggtctac cgcgagacga tcgacaacgt cgtcggcgtg cttcactcgc gcgatctgtt
     3781 ccaggcgtgg aaggccggcg aagatgatcg ggcggtggcg cactacctgc gtccggccgt
     3841 gttcgtgccg gaaaccctgt ccgcggccga gctgctggcc gagatgcgcc agcggacgca
     3901 gatcgcgttg gtcgtcgacg agtacggcgg tgtggcgggc ctgatcacgc tcgaggacgt
     3961 gctggaggag atcgtcggcg acatccgcga cgagcacgat agcgaagagg
//
LOCUS       DASPGN010000271        13023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1101728, whole genome shotgun sequence.
ACCESSION   DASPGN010000271 DASPGN010000000
VERSION     DASPGN010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 13023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13023
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101728"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="VD788_07920"
     CDS             <1..1552
                     /locus_tag="VD788_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76231.1"
                     /translation="FLDEVSHRFMHFSEPRLAGVDAADVRRFGRAVERAYAYQDRLLG
                     ELLQAAAPETTVIVLADHGFLNGPDRPPGETADVEDKPGRWHRRFGAFVAAGPPIEPG
                     RLAPVSLLDVAPTTLYLAGLPVPNDMDGRVLDELIRPEFRDRFPVTRIATYEIPPRAT
                     GVEHAAVSPAEREMIESLRSLGYIGGRSTDRPGGTLVTTHVNLAASLLGAGELERAEA
                     EIEAALDIEADYPPARLLLFNLQYRQRRYDEAIATGVGLLDEPELPRGRFALRLARAY
                     SAAGRRTEGVAMLKRRIADGQQRLAAVLAVLLYDERDLDGAERAARLALELDPLDEVA
                     IDVIFDIARETNRVDRIEPLLEAALRRNPRSPAHLNYLSFVRERAGATDEAVRLLEHA
                     LEADPGDAATLANLGRIHLQQGRYDAAASLLERAVAASPSNVTARMHLGSALAGGGRL
                     DDAIVVYEALAGDSPPTPPLCNALGQAHAAGGDLLRAAAWFRRSLELDPGQPEIRRRL
                     SQVEARLE"
     gene            1549..2772
                     /locus_tag="VD788_07925"
     CDS             1549..2772
                     /locus_tag="VD788_07925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV76232.1"
                     /translation="MTVARDKARALALLMLAELLAMSLWFSASAVVPQLTAEWGLDLS
                     EQAWLTMSVQAGFVAGALLSAWLNLADRIAAHRLLAAGALGGAVANGLVVLVPGPPAA
                     LVLRFATGFALAWIYPPGMKIVASWTSRDRGLGIGLLVGALTIGSAAPHLINALPLAG
                     GEAGTPPWRPVVLTASGSALLAAAIAAAWVRSGPRLPAASRFHWRYAIAALADQPTRL
                     ANIGYLGHMWELYAMWAWVPMLLLDSYRSAGLSELSARYAGFAVVAIGAPGCALAGKL
                     ADRYGRTIVSAASLALSGACCLCAGVVLESPVLLTAICLVWGSAVIADSAQFSAAVSE
                     LSDPAHVGTALTVQTCLGFLLTMVTLWLIPQVARSFGWIWAFALLALGPAVGAAGMMR
                     LRSMPAASRMASGHR"
     gene            2936..9559
                     /locus_tag="VD788_07930"
     CDS             2936..9559
                     /locus_tag="VD788_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HXV76233.1"
                     /translation="MHPRTPRRKPIHGACLLLAALWLGPAAGQTVFVDDDNCPGPGSG
                     TDLDPYCLIQDAICTLNGSGGGTVMVRPGEYNESLRMFPGVSVISTDGPAVTTIDADG
                     KPCTTGLCQPSANNLTCSTVVWGAGATPADRLEGFRITGGGGLFREFGGGDPPVALAG
                     GGIFVFSSSPTITNNEILANALSNPNNVKDFWGGGIYLGGGSYLAPITPVISHNLIQE
                     NVANPPGGQNRNTQTQGIGGGIYAATYTAPIVEGNTIRSNMAGDTNRLNQIGGGGGIA
                     IYSVSPMSEPRISSNLIQDNVGADFGGGISFGQAYALVSGLPVYYPSLGLVENNVIEL
                     NRSFSGGGLNTATTRALIRNNTIADNVADFGAGAATGTSGNIGDQATFVNNILAFNTS
                     LLYGAGGLGVYYSQPTIAYNDFFGNFPDDIDGDRGEADVIGFDGNISTDPRFVSREPG
                     SRDLHLTVGSGAVDAGDDLSAPATDIEGVARPLDGDANQLPTVDMGAYELFVDSDGDG
                     LFDPSDPDDDNDGVTDLDDCAPFDHVVSVIAPPVGSTLLAGPGASGIEWTWQAGVQAR
                     VFNVYRGNFAPLQNWSYGVTCLIAETPANRFTDPQIPIADHGYYYLIAAANGCGESRA
                     GVSTAGEIFADPSCPSAGSDSDGDGVPDRTDNCPFDPNPSQADPDVDFRGNPCDDDDD
                     GDGLADGEDNCPLDPLATQADGDDDGVGDACDNCPLDGNSSQTDTDADGDGDACDGDD
                     DDDGFGDLVDNCPLTADPTQADGDGDDVGNVCDNCPSTVNSGQADGDGDGAGDVCDVC
                     PADPDDDGDGDGHCADDDNCPLAANANQADGESDGVGDVCDNCPSVSNASQTDGDGDG
                     AGDVCDPCPADPDDDIDGDGFCGDVDNCPFVTNAQSDQDGDGVGDLCDNCPNDANSDQ
                     ADGDGDGIGDVCDLCPDADADGVCDVDDNCVLDPNPTQQDTDADTVGDACDNCTDSDR
                     DGFGNPGFALNICAPDNCPNAANASQSDQDLDGQGDACDICPVDPENDIDGDTRCGNV
                     DNCRTISNSNQLDSDLDGVGNVCDNCPGNANTNQSNVDGDGRGDVCDPCPGDAADDID
                     GDGVCADFDNCPTVPNSNQQDVDFDGIGEACDNCPGAGNPAQTDSDLDGTGDVCDTCT
                     DVDGDGFGAPGATSCGIDNCVSVANPGQANGDADALGDVCDPCPADALNDVDQDTICG
                     DVDNCPTTANTDQANVDQDATGDLCDDDIDGDSLLNGADNCPDVANFGQTDGDGDGTG
                     DPCDNCAATFNTGQEDADGDGIGNVCDACLDDPDNDIDADGLCGNLDNCPLINNTDQS
                     DDDLDGLGDPCDPCPTDPDIDGDAICNDDRVLVESEIANEIVLVEASQVNQIVLVEAG
                     STMRFLDNTGDPGIAMNWTAESFDDSAWPTGSYGVGYERSTGGARSLIATEVADGAFS
                     VYTRATFNIADVTQIDSLFLGADYDDGYIAWINGVEVFRSPEMPADPISWNTNAGNHE
                     SSNGITPLYEPYQDISNAIQQLHNGSNVVAIGLWNNGAPASGDLVIVPQLVANRVAVS
                     NMSYLANNADPGIGLSWTSEAFDDSSWTDGAYGIGYELSAGGATNLIHTPVPSDTLSV
                     YTRARFTIDDVGIVQNVVLGADYDDGFIAWINGVEVYRSPEMPAGDPLWNSDPLGHES
                     SNDPLPNYNPLIDVTAAAEAVLHDGVNVLAIGVWNREPSNPPSSDLVLVPKLSINRFT
                     STPVSFLANTSDPGIGQSWTQFAYDDSSWLSGPYGIGYEATGHGARNLIQTTVPSTSF
                     SVYTRARFTIQDLSSVHRVFLGADYDDGYTAWINGVEVFRSREMPGGTLSWNTPVNLH
                     ESSNGTRPNYNPLADITFAGLLALEQGDNVLAVGVWNSGAPTSTDLVIAPRLSVDGGA
                     VDNCTNAFNPNQLDSDGDGLGDACDPDDDNDLLADVIDNCRFTPNTDQDDADQDDVGD
                     VCDNCNATANPTQLDADQDGQGDACDVCPADPSDDADADTVCGDVDNCPSVSNLDQSD
                     ADGDGLGNLCDNCINDDNPQQDDADDDGLGDVCDDCPIDPDNDQDGDTLCAEADNCPA
                     VSNLDQTNSDADPFGDACDCRPLDPDIAVVSPPVDTLRMDKQGGVRLVWTDVGHVSIY
                     DVASGLLSELAVDGGVGAATCLVDDVAGTSVLDSQGNPPPGEALYYLLRGENVCGKGS
                     WGFDSGATERLLDVDCP"
     gene            complement(9603..11696)
                     /locus_tag="VD788_07935"
     CDS             complement(9603..11696)
                     /locus_tag="VD788_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HXV76234.1"
                     /translation="MTAAPDRRCALRFGPTLLALLALAAAGRPTDGAVACPEGNLLER
                     ARVSSSYDAYHVERLTDGIVAPEGEHWLSDRNSLFTTLGRVTFELPRTTRVNAVYLQG
                     DSNDSYRISVSEDGTRWQPLGEVPAVPGPGVRRRYAAEFDHELRYLRVEPGTGDGDFA
                     IAEIAAYCGVPEPWPPSFATRTGTVRDAAWAASRQRRLALWKIAYGVAGGLVLLGVAA
                     RRRLPPRLETALLVAVGLAGALAWTNFGTFHGPNVLHHHDVFHYVMGSKYFAENGYSG
                     LYHCTEVAEAELGRREFVERRVIRNLTTNELEPASIVLDDTQRCRSAFSAARWDEFKR
                     DVEFFRAGVYPGSWGTIFRDHGYNATPVWTMAGGRIANLDALSRRPVWLAAIDFALYA
                     GLFAMIAWAFGLEVAALGALIWGVGLPWQYYWTGGAFARVPWLALSVAAICLARRLRF
                     GAAGFALTSAALLRVFPALLVLGPMMVGARDALRAGRPARPTIRAAAGALVALVLLLP
                     LSAWAGGGFGVWTEFRDNLRKHSASPLGNDVGLPAIFAWSASNTQAEVLDGRLDRFAR
                     WEQARRETFAARRPAYLAAALAVLGLFAVFVLRYARSEWEQLAAGTLPMLALAELASY
                     YLIVLLLLAPFAARRPARVVIFVLTVVASQLVSLTDPSVDTLFFIQSLLFVGCAIAIV
                     ADVIRVRRRPTARQA"
     gene            11789..13018
                     /locus_tag="VD788_07940"
     CDS             11789..13018
                     /locus_tag="VD788_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HXV76235.1"
                     /translation="MKRDFERLAEIGSTGDGGVHRPALSDSHLEARHWFAELARREGF
                     ELRVDGAGNHSARLPCGRAGAPTLLLGSHLDSVPHGGRFDGALGVVAALEVLRAVRQA
                     GVERVVDLEAIDFTDEEGTWVCLLGSRAVAGTLSRTEFDRPRGSRQELTERMRVAGMT
                     ESGALAAARDPATLLAFLELHIEQGPRLLSSGADVGIVDSIVGIGSFDLQFNGRADHA
                     GTTPIDRRRDAGRGAAATIVEAFELARREFSDCTVNFGRMVLTPGAYNIVPAHAELAV
                     EFRAPETVRLHEFERELTALARRRAESLQLDLCIIPGPTIAAVPCAPGVRQLFGEACR
                     ALGLSAIDLSSGAGHDTGTMAGLCPAGMIFVPSVGGSHSSREYAEWQACIDGTNTLLL
                     ATLALATEAAGYKETQR"
BASE COUNT         2131 a         4553 c         4508 g         1831 t
ORIGIN      
        1 gttcctcgac gaggtgagcc atcgcttcat gcacttcagc gagccgcgcc tcgccggcgt
       61 cgacgccgcc gacgtgcggc gcttcggccg cgccgtcgag cgggcctacg cgtaccagga
      121 ccgcctgctc ggcgagctgc ttcaggcggc cgcgccggag acgacggtca tcgtgctggc
      181 ggatcacggc ttcctcaacg gaccggaccg gccgccgggc gagaccgccg acgtcgagga
      241 caagccgggt cgctggcacc ggcgcttcgg tgccttcgtc gccgcgggcc caccgatcga
      301 gcccggccgg ctcgcgccgg tctcgctgct cgacgtcgcg ccgaccacgc tgtacctcgc
      361 gggcctgccg gttcccaacg acatggacgg tcgcgtgctc gacgagctga tccggcccga
      421 gttccgcgat cggttccccg taacccggat cgcgacctac gagatcccac cgcgcgcgac
      481 cggcgtggag cacgccgcgg tctcgccggc cgagcgcgag atgatcgaga gcctgcggtc
      541 gctcggatac atcggcgggc gctcgacgga caggccgggc gggacgctgg tgacgacgca
      601 cgtcaacctc gccgcgtcgc tgctcggggc gggggagctc gagcgtgccg aggccgagat
      661 cgaggcggcg ctcgacatcg aggcggacta cccgccggcg cgactgctgc tgttcaacct
      721 gcagtaccgc cagcgccgct acgacgaggc gatcgcgacg ggggtcggtc tgctcgacga
      781 gcccgagctg ccgcgcggcc gcttcgccct gcgactggca cgcgcctact cggcggcggg
      841 ccgccggacc gagggggtcg cgatgctgaa gcgccgcatc gccgacgggc agcagcgatt
      901 ggccgcggtg ctggccgtgc tgctgtacga cgagcgcgat ctcgacggtg ccgagcgagc
      961 ggcgcgtctg gcgctcgagc tcgatccgct cgacgaggtc gcgatcgacg tgatcttcga
     1021 catcgcgcgc gagacgaacc gggtcgatcg gatcgagccg ctgctcgagg ccgcgttgcg
     1081 gcggaatccg cgctcgcccg cgcacctgaa ctacctgtcg ttcgtccgcg agcgggccgg
     1141 cgcgaccgac gaggccgtgc ggttgctcga gcacgcgctc gaggccgatc cgggcgacgc
     1201 ggcgacgctg gccaacttgg ggcggatcca cctgcaacag ggccgctacg acgcggccgc
     1261 gtcgttgctc gagcgcgcgg tcgccgcgag cccgagcaac gtgaccgcgc ggatgcacct
     1321 cggctccgcg ctcgccggcg ggggacgcct cgacgacgcg atcgtggtct acgaggcact
     1381 ggccggggac agcccgccga cgcccccgtt gtgcaatgcg ctggggcagg cccacgcggc
     1441 cggcggagac ctgctgcgcg ctgcggcgtg gtttcgccga tcgctcgagc tcgacccggg
     1501 ccagcccgag atccgccgcc ggctgagcca ggtcgaggcg cggctcgagt gaccgtcgcg
     1561 cgggacaagg cgcgagcgct cgcgctgttg atgctggccg agctgctggc gatgtcgctg
     1621 tggttttcgg cgtcggccgt cgtcccccaa ctcaccgccg agtgggggct cgacctgtcg
     1681 gaacaggcgt ggctgacgat gagcgtgcag gccggattcg ttgccggtgc gctgctgagc
     1741 gcgtggctca acctggccga ccgcatcgcg gcccaccgcc tgctcgccgc cggcgcgctc
     1801 ggcggcgccg tcgccaacgg actcgtcgtg ctggtgcccg ggccgcctgc ggcgctcgtg
     1861 ctgcgcttcg ccaccggctt tgcgctggcg tggatctacc caccggggat gaagatcgtc
     1921 gcgagctgga cctcgcgcga tcgcggtctc ggcatcggtc tgctggtcgg cgcgctgacg
     1981 atcggctcgg cggcgccgca cctgatcaac gcgttgccgc tggcgggcgg ggaggccggg
     2041 acgccgccgt ggcgtccggt cgtgctgacg gcatccggct cggcgttgct cgccgccgcg
     2101 atcgccgccg cctgggtccg ctccggtcca cggctgccgg cagcgagtcg cttccactgg
     2161 cgctacgcga tcgccgcgct ggccgaccag ccgacacgtt tggccaacat cggttacctc
     2221 gggcatatgt gggagctcta cgcgatgtgg gcctgggtgc cgatgctgct gctcgacagc
     2281 tatcgcagcg cgggcttgag cgagctgtcg gcgcgctacg ccggtttcgc ggtcgtcgcg
     2341 atcggcgcac cgggatgtgc gttggccggc aagctcgccg accgctacgg tcgaacgatc
     2401 gtctccgccg cgagcctggc gttgtcgggc gcatgctgtc tctgcgcggg agtcgtgctc
     2461 gagtcgccgg tgctgctgac ggcgatctgc ctggtgtggg gctcggcggt gatcgcggac
     2521 agcgcgcagt tctccgccgc ggtcagcgag ttgtccgacc cggcccacgt cggcaccgcg
     2581 ctgaccgtcc agacctgcct cggtttcctg ctgacgatgg tcacgctgtg gttgatcccg
     2641 caggtcgccc gctcgttcgg ttggatctgg gcgttcgccc tgctcgcgtt ggggccggcc
     2701 gtcggcgccg cgggcatgat gcggctccga tcgatgcccg cagcatcccg catggcctcc
     2761 ggccaccgct gaccgaaagt ccgccgaatg gagcaccgcg gggcctgcgg tggggagatc
     2821 agcagaatcg gacgcaccct ccgcgtcggg ccgattgccc cactcccgac gcaaccgtat
     2881 aaataaggac ctgcgccgga ccgcccgtgg gtgacggaga acggctgaag gggtcatgca
     2941 tccgcgtacg ccgcgtcgaa agccgatcca cggcgcctgt ctgttgctgg ctgcattgtg
     3001 gctgggcccc gccgccggtc agacggtgtt cgtcgacgac gacaactgcc ccgggccggg
     3061 gagcggtacc gacctcgatc cgtattgcct gatccaggac gcgatctgca cgttgaacgg
     3121 ctcgggcggc ggcaccgtga tggtgcgccc gggcgaatac aacgagtcgc tgcggatgtt
     3181 ccccggggtc agcgtcatct cgaccgacgg gcccgcggtg acgacgatcg acgccgacgg
     3241 caagccgtgc accaccgggt tgtgccaacc cagcgcgaac aatctgacct gctcgaccgt
     3301 ggtctggggc gcgggtgcga cgccggccga tcggttggag ggtttccgca tcaccggtgg
     3361 gggcgggttg ttccgcgagt tcggcggagg ggatccgccc gtcgcgctgg ccggcggcgg
     3421 gatcttcgtc ttcagcagct cgccgacgat caccaacaac gagatcctgg ccaacgccct
     3481 gtccaatccg aacaacgtca aggacttctg gggtgggggg atctacctcg gcggcggctc
     3541 ctatctcgcg ccgatcacac cggtcatcag ccacaacctg atccaggaga acgtcgccaa
     3601 cccccccggc ggtcagaatc ggaacaccca gacccagggg atcggcggcg ggatctacgc
     3661 ggcgacctac accgcgccga tcgtcgaggg caatacgatc cgcagcaaca tggcggggga
     3721 caccaaccgg ctgaaccaga tcggtggcgg cggtggcatc gcgatctaca gcgtgtcgcc
     3781 gatgagcgag ccgcgcatct cgtccaatct gatccaggac aacgtcggcg ccgatttcgg
     3841 cggcgggatc tccttcggtc aagcgtacgc cttggtctcg gggttgccgg tctactaccc
     3901 gtcgctcggt ctggtcgaga acaacgtcat cgagctgaat cgcagcttca gcggcggcgg
     3961 cctgaacacg gcaaccacgc gcgccctgat ccgcaacaac acgatcgccg acaacgtcgc
     4021 cgatttcggc gccggcgccg ccacgggcac cagcggcaac atcggggacc aggcgacgtt
     4081 cgtcaacaac attctcgcct tcaatacgtc gttgctctac ggggcgggcg gcctcggcgt
     4141 ctactactcg cagccgacga tcgcctacaa cgatttcttc ggcaacttcc cggacgacat
     4201 cgacggcgat cgcggcgaag cagacgtcat cgggttcgat ggaaacatct cgaccgaccc
     4261 gcgcttcgtc agccgcgaac ccgggagccg cgacctgcac ctgaccgtcg gcagcggcgc
     4321 ggtcgacgcc ggcgacgacc tgtcggcccc agccaccgac atcgagggag tcgcccgccc
     4381 gctcgacggc gacgcgaacc agctcccaac ggtcgacatg ggtgcttacg agctgttcgt
     4441 ggactcggat ggcgacgggt tgttcgaccc gagcgacccg gacgacgaca acgacggcgt
     4501 caccgacctg gacgattgtg cgccgttcga tcacgtggtt tccgtgatcg ctccgccggt
     4561 ggggtcgacg ctgctcgccg gccccggcgc ctccggcatc gagtggacgt ggcaggccgg
     4621 cgtccaggcc cgcgtgttca acgtctatcg cgggaacttc gcgccactgc agaactggag
     4681 ctacggcgtc acctgcctga tcgccgagac gcccgcgaac cgtttcaccg acccgcagat
     4741 cccgatcgcg gatcacggct actactacct gatcgccgcg gccaacggct gcggcgagtc
     4801 gcgcgccggc gtgtcgacgg ccggagagat cttcgccgat ccgtcgtgcc cctccgccgg
     4861 ctccgacagc gacggcgacg gcgtgccgga ccgaacggac aactgtccgt tcgaccccaa
     4921 tccgagtcag gccgatccgg acgtcgattt tcgcggcaac ccgtgcgacg acgacgacga
     4981 tggcgacggt ctcgccgacg gcgaggacaa ctgcccgctc gacccgctgg cgacccaggc
     5041 cgacggcgac gacgacggcg tgggcgacgc ctgcgacaac tgcccgctcg acgggaactc
     5101 gagccagacc gacaccgacg cggacggcga cggggacgcc tgcgacgggg acgacgacga
     5161 tgacggcttc ggcgacctcg tcgacaattg cccgctgacc gccgacccga cgcaagccga
     5221 cggggacggc gacgacgtcg gcaacgtgtg cgacaactgt ccgtcgacgg tgaactccgg
     5281 ccaagccgac ggcgacggcg acggtgccgg cgacgtgtgt gacgtctgtc ccgcggaccc
     5341 cgacgacgac ggcgacggcg acggacattg cgcggacgac gacaactgcc cgctggcggc
     5401 caacgcgaac caggccgacg gtgagagcga cggggtcggc gacgtatgcg acaactgccc
     5461 gtcggtgtcg aacgcgagcc agaccgacgg ggacggcgac ggcgctggcg acgtttgcga
     5521 cccgtgtccc gcggatccgg atgacgacat cgacggtgac ggtttctgcg gcgacgtcga
     5581 caactgcccg ttcgtcacca acgcccagtc cgatcaggat ggcgacggcg tcggcgacct
     5641 gtgcgacaac tgccccaacg acgccaactc cgaccaggca gacggcgacg gggacgggat
     5701 cggcgacgtc tgcgacctgt gcccggacgc cgacgcggac ggagtgtgcg acgtcgacga
     5761 caattgcgtg ctggacccga acccgaccca gcaggacacc gacgccgaca cggtcggcga
     5821 cgcctgcgac aactgcaccg attcggaccg ggacggcttc ggcaaccccg ggttcgcgct
     5881 gaacatctgt gcgcccgaca actgcccgaa cgccgccaac gccagtcaat ccgatcagga
     5941 cctcgacggc cagggtgacg cttgcgacat ctgcccggtc gaccccgaga acgacatcga
     6001 cggcgacacc cgctgcggca acgtcgacaa ctgtcgcacg atcagcaaca gcaatcagct
     6061 ggactcggat ctggacggcg tcggcaacgt ctgcgacaac tgtccgggga acgccaacac
     6121 caaccaatcg aacgtcgacg gcgacggccg cggcgacgtc tgcgacccgt gtccgggaga
     6181 tgccgctgac gacatcgacg gcgacggcgt ctgcgccgac ttcgacaatt gcccgacggt
     6241 gcccaacagc aatcagcagg acgtcgattt cgacggcatc ggcgaggctt gcgacaattg
     6301 ccccggcgcg ggcaatccgg ctcagaccga ttccgacctc gacggtaccg gcgacgtctg
     6361 cgacacctgc accgacgtcg acggcgacgg gttcggtgcc ccgggggcga cgagctgcgg
     6421 gatcgacaac tgcgtgagcg tagccaaccc gggccaagca aacggcgacg ccgacgcgct
     6481 cggcgacgtc tgcgaccctt gccccgccga cgcgctgaac gacgtcgacc aggacacgat
     6541 ctgcggcgac gtcgacaact gcccgaccac ggcgaatacc gaccaggcca acgtcgacca
     6601 ggacgcgacc ggcgacctgt gcgacgacga catcgacggc gacagcctgc tcaacggagc
     6661 cgacaactgc cccgacgtcg ccaacttcgg tcagacggac ggggacggcg acggcacggg
     6721 cgatccctgc gacaactgcg ccgcgacgtt caacacgggc caggaagacg cggacggcga
     6781 tggcatcggc aacgtctgcg acgcgtgtct cgacgatccc gacaacgaca tcgacgccga
     6841 cggactctgc ggcaacctcg acaactgccc gttgatcaac aacaccgatc agagcgacga
     6901 cgacctggat ggattgggcg acccgtgcga tccgtgcccg accgatcccg acatcgacgg
     6961 cgatgcgatc tgcaacgacg accgcgtgct cgtcgagtcg gagatcgcca acgagatcgt
     7021 gctggtcgag gccagccagg tcaaccagat cgtgctggtc gaggccggct cgacgatgag
     7081 gttcctcgac aacaccggcg atccggggat cgcgatgaac tggaccgccg agtcgttcga
     7141 cgactcggcg tggcccacgg gcagctacgg cgtcggctac gaacggagca cgggaggcgc
     7201 gcgcagcctg atcgcgaccg aggtcgccga tggggcgttc tcggtctaca ctcgcgcgac
     7261 gttcaacatc gccgacgtga cgcagatcga cagcttgttc ctcggcgccg actacgacga
     7321 cggctacatc gcctggatca acggcgtcga ggtcttccgt tcgccggaaa tgccggccga
     7381 cccgatctcg tggaacacca atgccggcaa ccacgagtcg agcaacggca tcacgccgct
     7441 gtatgaaccg taccaggaca tctcgaacgc gatccagcag ctgcacaacg gcagcaacgt
     7501 ggtcgcgatc gggctgtgga acaacggcgc gccggcgtcg ggcgacctgg tgatcgtccc
     7561 gcagctggtg gccaatcggg tcgccgtctc caacatgtcc tatctcgcga acaacgcgga
     7621 tccgggtatc gggctgagct ggaccagcga ggcgttcgac gattcgagct ggaccgacgg
     7681 cgcctacggg atcggctacg agctgagcgc cggcggcgcg accaacctga tccacacgcc
     7741 ggtgccgtcc gacacgttgt cggtctacac gcgcgcccgc ttcaccatcg acgacgtcgg
     7801 catcgtgcag aacgtcgtgc tcggtgcgga ctacgacgac gggttcatcg cctggatcaa
     7861 cggtgtcgag gtctaccgct cccccgagat gcccgccggc gatccactat ggaacagcga
     7921 tccgctggga cacgaatcga gcaacgaccc gctgccgaac tacaacccgc tgatcgacgt
     7981 caccgcggcg gccgaggcgg tgctgcacga cggcgtcaac gtgctggcga tcggcgtgtg
     8041 gaaccgcgag ccgtcgaatc caccgtcgtc ggacctggtt ctcgttccca aactgtcgat
     8101 caatcgcttc acctcgacgc cggtctcgtt cctggccaac acctcggacc cggggatcgg
     8161 gcagtcgtgg acccagttcg cctacgacga ttcgagctgg ctgagcgggc cctacggcat
     8221 cggctacgag gcgaccggcc acggcgcgag gaacctgatc cagacgacgg ttcccagcac
     8281 ttccttctcg gtctacaccc gcgcgcgctt cacgatccag gacctgtcgt ccgtccaccg
     8341 cgtcttcctc ggcgccgact acgacgacgg gtacaccgcc tggatcaacg gtgtcgaggt
     8401 cttccgctcg cgcgagatgc ccggcggcac gctgagctgg aatacgccgg tcaacctgca
     8461 cgagtcgagc aacggaaccc ggccgaatta caacccgctc gccgacatca ccttcgccgg
     8521 gctgcttgcc ctcgaacagg gcgacaacgt gctcgcagtc ggcgtgtgga actcgggcgc
     8581 gccgacctcg accgatctgg tgatcgcgcc gcggttgtcg gtcgacggcg gcgcggtcga
     8641 caactgcacc aacgccttca acccgaacca gctcgacagc gacggcgacg gcctgggcga
     8701 cgcctgcgac ccggacgacg acaacgatct gctggcggac gtgatcgaca actgtcgctt
     8761 cacgccgaac accgatcagg acgatgccga tcaggacgac gtcggcgacg tttgcgacaa
     8821 ctgtaacgcg acggcgaacc ccactcagct cgacgccgac caggacggac aaggcgacgc
     8881 gtgcgacgtc tgtccggcgg acccgtcgga cgacgccgac gcggacaccg tctgcggcga
     8941 cgtcgacaac tgtcccagcg tgtcgaacct cgaccagtcg gacgcggacg gtgacggtct
     9001 cggcaacctg tgtgacaact gcatcaacga cgacaacccg cagcaggacg acgccgatga
     9061 cgacggtctg ggcgacgtct gcgacgactg tccgatcgac ccggacaacg accaggacgg
     9121 tgatacgctc tgcgccgaag ccgacaactg tccggcggtg tcgaacctcg accagacgaa
     9181 cagcgacgcc gacccgttcg gcgacgcctg cgattgccgg ccgctcgacc ccgacatcgc
     9241 ggtcgtgtcg ccgccggtcg acacgttgcg gatggacaag cagggcggcg tccggctggt
     9301 ctggaccgat gtcggccacg tgtcgatcta cgacgtcgcc tcgggtctgc tgagcgagct
     9361 ggccgtcgac ggcggggtcg gcgcggcgac gtgtctcgtc gacgacgtgg cgggcacgag
     9421 cgtgctcgac tcgcagggca atccgccgcc gggcgaagcg ctgtactatc tgctgcgcgg
     9481 cgagaacgtc tgcgggaagg ggagctgggg cttcgactcg ggcgccaccg agcgcttgct
     9541 cgacgtcgac tgtccctagg agcctgtccg agtaccgcgc cgccgcaggc aatactcctg
     9601 gctcaggcct ggcgcgcggt cggtcgccgg cggacgcgga tcacgtcggc cacgatcgcg
     9661 atcgcgcagc cgacgaacag cagtgactgg atgaagaaca aggtgtcgac cgacggatcg
     9721 gtgagcgaga ccagctgact ggcgacgacc gtcagcacga agatcacgac gcgcgccggg
     9781 cgccgcgcgg cgaacggcgc gagcagcagc agcacgatca ggtagtacga ggccagctcg
     9841 gccagcgcca gcatcggcag cgtacccgcc gccagctgct cccactcgct tcgcgcgtaa
     9901 cgcagcacga agaccgcgaa cagccccagc acggccagcg ccgcggcgag ataggccgga
     9961 cgccgcgcgg cgaaggtctc ccgccgcgct tgctcccacc gggcgaaccg atcgagccga
    10021 ccgtcgagca cttcggcctg cgtgttcgac gcgctccagg cgaagatcgc gggcaggccg
    10081 acgtcgttgc ccagcggcga cgccgagtgc ttgcgcaggt tgtcgcggaa ttccgtccac
    10141 acgccgaacc cgccgccggc ccacgcgctg agcggcagca gcaggaccag cgcgacgagc
    10201 gcccccgccg ccgcgcgaat cgtcggacga gccggccgtc ccgcgcgcag ggcgtcacgc
    10261 gcccccacca tcatcggacc gagcacgagc agcgcgggga agacgcgcag cagcgcggcc
    10321 gaggtcaagg cgaaaccggc ggcgccgaag cgcagacgcc gcgccagaca gatcgcggcg
    10381 acggatagcg cgagccacgg gacgcgcgcg aacgctccgc ccgtccagta gtactgccac
    10441 ggaagaccga caccccagat caaagcgccg agtgccgcga cctcgagtcc gaaggcccac
    10501 gcgatcatcg cgaacagtcc ggcgtacaac gcgaaatcga tcgcggcgag ccacaccggc
    10561 cggcggctca acgcgtcgag attggctatc cggccgccgg ccatggtcca caccggggtc
    10621 gcgttgtagc cgtggtcgcg gaagatcgtc ccccacgagc ccgggtagac gccggcgcgg
    10681 aagaactcga cgtcgcgctt gaactcgtcc cagcgcgccg cggagaacgc gcttcggcaa
    10741 cgctgcgtgt cgtcgagcac gatgctcgcc ggctcgagct cgttcgtcgt caggttgcga
    10801 atcacccggc gctcgacgaa ctcgcggcga ccgagctcgg cttcggcgac ctcggtgcag
    10861 tgatagagac cggagtagcc gttctcggcg aaatacttcg accccatcac gtagtggaag
    10921 acgtcgtggt ggtgcagcac gttcgggccg tggaaggtgc cgaagttggt ccaggccaac
    10981 gcgccggcga ggccgaccgc gacgagcagc gccgtttcga gccgcggcgg caagcgtcgc
    11041 cgggcggcga cgccgagcag gaccagcccg cccgccacgc cgtaggcgat cttccacagc
    11101 gcgagtcgtc gctggcgcga ggcggcccac gccgcgtcgc gaaccgtccc ggtgcgggtc
    11161 gcgaacgacg gtggccacgg ttcggggacg ccgcagtagg cggcgatctc ggcgatggcg
    11221 aagtcgccgt cgccggtccc gggctcgaca cgcaggtagc gcagctcgtg gtcgaattcg
    11281 gcggcgtagc gccggcggac gcccggcccg gggacggccg gcacctcacc gagcggttgc
    11341 catcgcgtgc cgtcctccga aacggagatg cggtagctgt cgttcgaatc gccctgcagg
    11401 tagaccgcgt tgacgcgcgt cgtgcgcggc agttcgaagg tcacccggcc gagcgtggtg
    11461 aagagcgagt tgcggtcgct caaccagtgc tcgccttccg gtgcgacgat gccgtcggtc
    11521 agccgttcga cgtggtatgc gtcgtaggac gacgacacgc gggcccgctc gagcaggttg
    11581 ccctcggggc aggcgacggc gccgtcggtc ggccgcccgg cggcggcgag ggcaagaagc
    11641 gccaacagcg tcgggccgaa acggagcgcg catcttcgat ccggggccgc ggtcatcggg
    11701 ctgggagcat ataccaacgt ggtacagtct tcccttcgtg cagtacgggc gtgtaacgat
    11761 gaaccggtca cgggtcgacg gggacaggat gaaacgcgac ttcgagcggt tggccgagat
    11821 cggctcgacc ggcgacggcg gcgttcaccg gccggcactg tccgacagcc atctcgaagc
    11881 gcgtcactgg ttcgctgagc tggcgagacg cgagggcttc gagctccgcg tcgacggcgc
    11941 gggcaaccac agcgcgcggc tgccgtgcgg gcgcgccggt gcgccgaccc tgttgctcgg
    12001 atcgcatctc gactccgttc cgcacggcgg tcggttcgac ggtgcgctgg gcgtcgtcgc
    12061 cgccctcgag gtgctgcgag cggtacgcca ggccggcgtc gagcgggtcg tcgacctcga
    12121 ggcgatcgac ttcaccgacg aggaggggac ttgggtctgc ctgctcggta gccgtgcggt
    12181 ggcgggcacg ctctcccgga ccgaattcga ccggccgcgc ggtagccggc aggagctgac
    12241 cgaacggatg cgggtggccg ggatgaccga gtcgggcgcg ctggcggcgg cgcgcgaccc
    12301 ggcgacgctg ctcgccttcc tcgagcttca catcgagcag gggccgcgcc tgctgtcgag
    12361 cggggccgac gtcggcatcg tcgactcgat cgtcggcatc ggttcgttcg acctgcagtt
    12421 caacggccgc gccgaccacg ccggcacgac gccgatcgac cgacgtcgcg acgcgggacg
    12481 cggcgcggcg gcaacgatcg tcgaggcgtt cgagctggcc cggcgcgagt tctccgactg
    12541 cacggtcaac ttcggccgga tggtgctgac ccccggggcc tacaacatcg tacccgcgca
    12601 cgccgagctg gccgtcgagt tccgggcgcc ggagaccgtc aggctccacg agttcgagcg
    12661 cgagctgacc gctctcgcgc ggcgccgcgc ggagagtttg cagctcgacc tgtgcatcat
    12721 cccggggccg acgatcgccg ccgttccgtg tgcaccgggc gtccgccagc tgttcggcga
    12781 ggcgtgccgc gcgctcggtt tgtcggcgat cgatctgtcg tcgggcgccg ggcacgatac
    12841 cgggacgatg gccgggctct gtccggcggg aatgatcttc gtaccgtcgg tcggtggcag
    12901 ccactcgtcc cgcgagtacg ccgagtggca ggcatgtatc gacgggacca acacgctgtt
    12961 gctggccacc ttggcgctgg ccacggaggc ggccgggtat aaggagacgc agagatgaga
    13021 acc
//
LOCUS       DASPGN010000272         5085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_399394, whole genome shotgun sequence.
ACCESSION   DASPGN010000272 DASPGN010000000
VERSION     DASPGN010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399394"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VD788_07945"
     CDS             complement(<1..1274)
                     /locus_tag="VD788_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXV76236.1"
                     /translation="MPGRLPNKTIVAIALPLVLLSVALGLSIRSVPRRAIGLLDSPLR
                     GDRVRGPGLCLVIPGLESLEVVDPYTRSGELAYRTVEGSRIGLELGFRLRLTEDGARR
                     LLAEEDAGPPRQRLAIALDRRIVDRLRSVSPSSLPETDARAQAALADLLADYGTVEGG
                     VELSLGGDGAVAASIDAARERQRIRELVRATGVRILLVGVDGMDWQIAEPLIERGELP
                     NVASLRQRGAWGEVQSLLPMLSPLLWTSVATGVTPDKHGVVDFLETDGSTGLQRPVTA
                     RSRKVRALWDTFSELGRSVDVVAWWASWPAEEVAGHLVSDRVAYSLFDVAAPDGDHRL
                     TWPASYYDEIRGRLVNDEEIGYREISRFVDVTREEFEAARRRIAVDRDAAYREPVNHL
                     TRVLASTESYHRITLELVGRKQADFAAVYYQFL"
     gene            1390..1926
                     /locus_tag="VD788_07950"
     CDS             1390..1926
                     /locus_tag="VD788_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV76237.1"
                     /translation="MEAVWSLPAAGGALREVAEEREAAGFPELYRRHGRALYGTALRL
                     TGRPEDAEDVVQDVFLAWHRRRPDLPPEQLGRWLTRVAVNAALDRLRRSRRWRTEEFD
                     ESRPGIDGAPAELAVDLERAVARLPEKARLVFLLHDVEGLAHRELAEVLGATVGTTKS
                     QLFRARTMLKEAMKRGRR"
     gene            1923..2582
                     /locus_tag="VD788_07955"
     CDS             1923..2582
                     /locus_tag="VD788_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76238.1"
                     /translation="MSCERFRDAIVGDDPTGLAEAERHAAGCGDCAAWIDEQRRLAVA
                     VARWKAAAPAPPAELERRIAAAIEKDGGARQPGERRVAGQRLAWWGAVAASLVLALWL
                     APRWWSPAPTAIGEVERLIAEADRARADYARSIAELERRAMPILARAGDPDLDPLRAA
                     GLLRYRDRLLHLDSVITEAELFLEQNPGHAGGHNVLASAYQQKQQVLGELIRLESGDA
                     S"
     gene            2579..4093
                     /locus_tag="VD788_07960"
     CDS             2579..4093
                     /locus_tag="VD788_07960"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXV76239.1"
                     /translation="MRVSSAARVALAASLTLVPVVPLVHARDAFDREDAFRRSFSRAF
                     DSAGRKVVIDGAISELTIERSADSSVRVDAELLFWSNVESYREQVEREFDVRTQETAE
                     AITIGVELPDFDGRRRGNIELRYEVTMTVTVPRGTPLDVSNRYGGVRVAGVDGPTRIV
                     NNSGAIELTGAAGDVRLESRYGEILVTDVDGNLYANGSSGTLEVRGVTGDAELGNQYA
                     DIVAEQIGGRLSAQTSSGGVRVAGIGGDARIVASYADVEAADVGGRLDVDASSGAVRV
                     RDVKLGARLRSSYAGVEAERIGGDAEIVASSGTARVIDVGGKLDLRGSYGDVAVRGTG
                     GPATIEVDSGSVTAEKVGGSLEIDSSYGTVRVRGVRGSLRVKSDSTGVLAAEIAGAVE
                     IETSYAGVEIDGAGGAIEVNNESGEVIVGGLTGDALSAEHRVRTTYADVRFHWPADRG
                     ASYALESGYGSVTCELPGKRTTSGSRSKFEGRSGDGTARISLTAESGSVWLRGE"
     gene            complement(4159..>5085)
                     /locus_tag="VD788_07965"
     CDS             complement(4159..>5085)
                     /locus_tag="VD788_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76240.1"
                     /translation="DDHNDTANGVTDRPVVSDPPGTEIHQATLAYHGLPDTRLEIGRR
                     EITLDDQRFVGNVAWRQNHQSFDALYVTQESLPRTKLTYAYVDNVNTVRTTDDPIHSH
                     LLNANVDLERYGTAVAYYYYLDFDASARSPFSSASFGVSWTDRFEAGEFELPLRVELA
                     EQREVGQNPNRIDAGYRRLELGASRAGASLTAGYELLEGSPADGQFNTPLATLFKFNG
                     WADKFLVTPADGLQDLYLVGEYGFSRYRALLAFHRFDADSGGDRYGSELDARMVYTAP
                     WDQQFVLSFAIFDGDVGFTDTNKLWIFSEYAF"
BASE COUNT          766 a         1709 c         1898 g          712 t
ORIGIN      
        1 aggaactggt agtagacggc ggcgaagtcg gcttgcttcc ggccgaccag ctcgagcgtg
       61 atgcggtggt agctctcggt cgacgcgaga acgcgcgtca ggtgattgac cggttcacgg
      121 taggcggcgt cgcggtcgac cgcgatccgc cggcgggcgg cttcgaactc ttcgcgggtg
      181 acgtcgacga agcgggagat ctcgcgataa ccgatctcct cgtcgttgac cagccggccg
      241 cggatctcgt cgtagtacga cgcgggccag gtcaggcggt gatcgccgtc gggtgccgcc
      301 acgtcgaaca gcgagtacgc cacgcgatcg ctgaccaggt gtccggccac ctcctcggcc
      361 ggccagctcg cccaccaggc gacgacgtcg accgaacggc cgagctcgct gaacgtgtcc
      421 cacaacgcac gcaccttgcg cgagcgcgcc gtcaccggtc gctgcagacc ggtcgatccg
      481 tccgtctcga gaaaatcgac gacgccgtgc ttgtccggcg tgacgccggt ggcgacgctg
      541 gtccacagca gcgggctcag catcggcagc aaggattgga cctcgcccca cgctccgcgc
      601 tgccgcagcg acgcgacgtt gggcagctcg ccgcgctcga tcagcggctc ggcgatctgc
      661 cagtccatgc cgtccacgcc gacgagcagg atgcgcacgc cggtcgcgcg caccagctcg
      721 cgaatccgct gccgctcgcg cgccgcgtcg atcgacgccg cgaccgcgcc gtcgccaccg
      781 aggctcagct cgacgccgcc ctcgacggtg ccgtagtccg cgagcaggtc ggccagcgcc
      841 gcctgcgccc gcgcgtcggt ttccggcagc gacgagggcg aaacggagcg caaccggtcg
      901 acgatccgcc ggtcgagtgc gatcgccagt cgctggcgcg gcggaccggc atcctcttcg
      961 gcgagcagac gccgggcgcc gtcctcggtc aagcgcaagc ggaagccgag ctcgagtccg
     1021 atccgcgacc cctcgaccgt gcggtaggcc aattcgccgc tgcgggtata ggggtcgacc
     1081 acctcgagcg actcgagacc gggtatgacg agacagagac ccggcccacg gacccggtcc
     1141 ccgcgcagcg gcgagtcgag cagaccgatc gcccgccgcg ggaccgagcg gatcgacaga
     1201 ccgagcgcga ccgacagcag cacgagcggc agcgcgatcg cgacgattgt cttgttgggg
     1261 agtcgaccgg gcataacgga atcgccccga aaacgggcgc ccggcaagga tagcaccggc
     1321 ggccggccgg cccgttccaa ccaaacccgc gccccgcgcg ttaaccgcac ggagaacgga
     1381 gagggaataa tggaggcagt ctggtcattg ccggccgcgg ggggggcgtt gcgggaggtc
     1441 gcggaagaac gggaagcggc aggcttcccc gagctctatc ggcgccacgg gcgagcgttg
     1501 tacggcacgg cgctgcggct gaccggacgc ccggaggacg cggaggacgt cgtgcaggac
     1561 gtgttcctgg cctggcaccg ccggcgaccg gacctgccgc cggagcagct cggacgctgg
     1621 ctgacccgcg tcgccgtcaa cgcggcgctc gaccgcctgc gtcgttcgcg acgttggcgc
     1681 accgaggagt tcgacgagtc gcgcccgggg atcgacggtg cgccggccga gttggcggtc
     1741 gatctcgagc gcgcggtcgc ccggctgccc gagaaagccc gtctggtctt cctgctgcac
     1801 gacgtcgagg ggctggccca tcgcgagctg gccgaggtgc tcggcgcgac cgtcggcacg
     1861 accaagtccc agttgttccg ggcacgaacg atgctgaagg aagcgatgaa gcgaggtcgg
     1921 cgatgagctg cgagcggttt cgagacgcga tcgtcggcga cgatccgacc gggctggccg
     1981 aggccgagcg ccacgcggcc ggctgcggcg attgcgcggc gtggatcgac gagcagcggc
     2041 ggctggccgt ggcggttgcg cgatggaagg cagccgcgcc ggcgccgccc gcggagctcg
     2101 agcgacgcat cgcggcggcg atcgaaaagg acggcggggc gcggcagccc ggcgagcggc
     2161 gggtggccgg gcagcgtctc gcctggtggg gcgcggtcgc ggcctcgttg gtgctggcgc
     2221 tgtggcttgc gccgcgctgg tggtcgccgg ccccgaccgc catcggcgag gtcgagcgat
     2281 tgatcgccga ggccgatcgc gcgcgcgccg actacgctcg ctcgatcgcc gagctcgaac
     2341 ggcgcgcgat gccgatcctc gcccgggccg gcgaccccga cctcgacccg ttgcgcgccg
     2401 ccgggctgct gcgttaccgc gaccggttgt tgcacctcga ctcggtgatc accgaggccg
     2461 agctgtttct cgagcagaac cccgggcacg ccggcggcca caacgtgctg gcgtcggcct
     2521 accaacagaa gcaacaggtg ctcggcgagc tgatccggct cgaatcgggg gacgcgtcat
     2581 gagagtctct tcggccgctc gcgtggcgct ggccgcgtcg ttgaccctcg tgccggtggt
     2641 gccgctggtg cacgcgcggg acgcgttcga tcgcgaggac gccttccgcc gctcgttctc
     2701 gcgcgccttc gactcggcgg gccggaaggt ggtgatcgac ggcgcgatca gcgagttgac
     2761 gatcgagcgc tcggccgact cgtcggtgcg cgtcgacgcc gagctgttgt tctggtcgaa
     2821 cgtcgagagc taccgggagc aggtcgaacg ggagttcgac gtgcgcacgc aagagacggc
     2881 cgaggcgatc acgatcgggg tcgagcttcc cgatttcgac ggccggcgcc gcggcaacat
     2941 cgagctgcgc tacgaggtga cgatgacggt caccgttccg cgcggcacgc cgctcgacgt
     3001 gtcgaaccgc tacggcggcg tacgcgtcgc cggggtcgac ggcccgacgc gcatcgtcaa
     3061 caactcgggc gcgatcgagc tgaccggcgc cgccggcgac gttcggctcg agagccgcta
     3121 cggcgagatc ctcgtcacgg acgtcgacgg caacctctat gccaacggtt cgagcgggac
     3181 gctcgaagtg cgaggggtga ccggcgatgc cgagctcggc aaccagtacg ccgacatagt
     3241 cgccgagcag atcggcgggc ggctttcggc ccagacctcg agcggcggtg tgagggtcgc
     3301 ggggatcggc ggcgatgcgc ggatcgtcgc ctcctacgcc gacgtcgagg ccgccgacgt
     3361 cggcgggcgg ctcgacgtcg acgcttcgtc gggcgcggtg cgcgtgcgcg acgtcaagct
     3421 cggagcgcgc ctacgctcgt cgtacgccgg cgtcgaggcc gaacggatcg gcggcgacgc
     3481 ggagatcgtg gccagctcgg gcacggcgcg ggtgatcgac gtcggcggga agctcgacct
     3541 gcgtggatcc tacggcgacg tcgcggtgcg cgggaccggc ggaccggcga cgatcgaggt
     3601 cgacagcgga agcgtgaccg cggagaaggt cggcggatcg ctcgagatcg attcctccta
     3661 cggcacggtg cgggtgcgcg gcgtgcgcgg ctcgctgcgc gtcaagtccg acagcacggg
     3721 cgtgctggcc gccgagatcg ccggcgccgt cgagatcgag acgagctacg cgggagtcga
     3781 gatcgacggc gcgggcggcg cgatcgaagt caacaacgag agcggggagg tgatcgtcgg
     3841 cggcttgacc ggcgacgccc tgtcggcgga gcatcgcgtg cgcacgacct acgccgacgt
     3901 ccgcttccac tggcccgccg accgcggggc gagctacgcg ctcgagtcgg gctacggcag
     3961 cgtcacctgc gagctgccgg gcaagcggac gaccagcggc tcgcgcagca agttcgaggg
     4021 ccgctcgggt gacggcacgg cgcgcatttc gttgaccgcg gaaagcggca gcgtctggct
     4081 gcgcggcgag tagcaccgcg cggacggcgg ttcgtaagcg cgcgggccgg tgcggtgacc
     4141 gggcccggcg cgtccaagtc agaacgcgta ctcggagaag atccacagct tgttcgtgtc
     4201 ggtgaagccg acgtcgccgt cgaagatcgc aaacgacagc acgaactgct ggtcccacgg
     4261 cgccgtgtag accatgcgcg cgtcgagctc gctgccgtaa cgatcgccgc cgctgtcggc
     4321 gtcgaagcgg tggaacgcga gcagcgcgcg gtaccggctg aagccgtact ctccgaccag
     4381 gtagagatcc tgcaggccgt cggcgggcgt gacgaggaac ttgtcggccc agccgttgaa
     4441 cttgaacagc gtggcgagcg gcgtgttgaa ctggccgtcg gccgggctgc cctcgagcag
     4501 ctcgtagccg gccgtcagcg acgcgcccgc acgcgacgcg ccgagctcga gccgccggta
     4561 gccggcgtcg atcctgttcg ggttctgccc gacctctcgc tgctcggcca gctcgacgcg
     4621 cagcggcagc tcgaactcgc ccgcttcgaa ccggtcggtc cagctcacgc cgaacgacgc
     4681 cgacgagaac ggcgagcgcg cgctcgcgtc gaagtcgagg tagtagtagt aggcgacggc
     4741 cgtgccgtaa cgctcgagat cgacgttggc attcaacaaa tggctgtgga tcggatcgtc
     4801 ggtggttctc acggtgttga cgttgtcgac gtaggcgtag gtcagcttgg tccgcggcag
     4861 cgattcctgc gtgacgtaga gcgcgtcgaa cgactgatgg ttctgccgcc aggcgacgtt
     4921 gccgacgaat cgctggtcgt ccaacgtgat ctcgcgccgg ccgatctcga gccgggtgtc
     4981 gggcagtcca tggtaggcca gcgtcgcctg gtggatctcg gtccccggcg ggtcgctgac
     5041 gacgggccgg tcggtcacgc cgttcgccgt gtcgttgtgg tcgtc
//
LOCUS       DASPGN010000273         6857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1335150, whole genome shotgun sequence.
ACCESSION   DASPGN010000273 DASPGN010000000
VERSION     DASPGN010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335150"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VD788_07970"
     CDS             complement(<1..863)
                     /locus_tag="VD788_07970"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV76241.1"
                     /translation="MSGTTKLAVAWFVVLGLTCVAAPWLGLRDPNAQPDTLVLRDLPP
                     LARAQAVRLADGGLRYAQAVRPLADGSVEVRRGRTTIVLARAELASDWNVRPWYPLGT
                     DGFGRDMLSRLVHGARVSLLVGLAAAAIALGLGTGIGLVAGVAGGWVDALLMRLTDVV
                     LAVPRLFLALLLVALYRPSLSATVLVLGTTTWMAAARLVRGEVLSLKQREFVSAARAA
                     GASPFRLGTRHLLPAVLGPVVVEGTLRVGDTILLESALSYVELGVRPPMASWGNLIAD
                     GRDSLLDAWWIA"
     gene            complement(860..1849)
                     /locus_tag="VD788_07975"
     CDS             complement(860..1849)
                     /locus_tag="VD788_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV76242.1"
                     /translation="MAWFVLRRLALSVPLVVGVATLVFALLETAPGSPTDLILGDRPL
                     PQEVRRQVERAYGLDRPAHERYARWVGALLVRGDLGWSISRSRPVAEVLARALPATLV
                     LTTAALGIHVLLGVTLGIVSATRRGRWPDRIVTLCSLTLYAMPPFWLGLMAILGLAFL
                     VPLFPASSMQSVGAEQWGAARRFADLLWHLALPAGVLAVSSAAAMTRFVRAGLIESMG
                     QEFVRAARARGVGGQRVVLAHALRGSLGPVVNLIGLSLPVLVSGSLVIEIVFAWPGMG
                     RLTYDAIMAKDLPVVLATTTISAFVVVLGSLIADLALAALDPRVRLGGGRAAP"
     gene            complement(1857..3509)
                     /locus_tag="VD788_07980"
     CDS             complement(1857..3509)
                     /locus_tag="VD788_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV76243.1"
                     /translation="MIHRWRRSWPGRLGVLLASAALVSVCACGGPPVERPGAGADDES
                     PRQGGTAVLGSISDVDSWNEYTSQQSFASNVLRRIYLRLADDRGTGLERPEEFAPSLA
                     TDWQVAEDGLAITFFLREARWSDGVPLTADDVRFTWQAQTEPAVPWPGAVNKRRITDV
                     EVVDPRTVRFHFDGRYPYQFADAVDGGILPRHRFGEIPFDEWMTHDWSQYSIGSGPFV
                     LSRHVPQQEIVLERNLHYFEPELPRLDRVVVRIVPDVLTLLTQLQAGEIDWIDGIPPR
                     EAERLADAASAVSIVPFDYPGYDFIGWNEARAPFDDAEVRRALTMAIDRAALVEELLY
                     GYGEVAGTPVPSGWWGADRSIRPLPYDPEGARRLLASRGFRTVGADGVERPGEVLTIE
                     ILTNNGNRLRRDALVKVQQQLDLVGVEATVHPLEAKALRHQVGAGDYDAFLGGWVFVG
                     RVDLAMLFGGGNLPPNGLNVVQYRSASLDELLDRLQRADSAIAMGQPLHEIQRQLHRD
                     QPYTLLYEAQRIAAHGPRLRGVEVDVPADPLAALERFWVDSP"
     gene            complement(3577..4005)
                     /locus_tag="VD788_07985"
     CDS             complement(3577..4005)
                     /locus_tag="VD788_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HXV76244.1"
                     /translation="MDAERPAWYAPGLRFECKENCGACCTNHDDYAYVYLVGDDVDRL
                     VAHLGIDEDEFLGRYTDLDDGQLVLRMDAPSCPFLDGTRCGVYAARPTQCSTFPFWHE
                     ALASRDSWERLKRFCPGIDQGPRHDLLTIRGQLARRPPDD"
     gene            complement(4014..>6857)
                     /locus_tag="VD788_07990"
     CDS             complement(4014..>6857)
                     /locus_tag="VD788_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV76245.1"
                     /translation="IVVAIGCAELVAVGWSIARRARPIDGRRASRVAVGVVTSLLLVG
                     GGFSLERRTRDLALERLRSEFVPLVRDQSARRSVALTAAVRGVAEAFGRGDEVGSPRS
                     SRPDVMAYEFWVSSPLFFNGYQSSLSFYNPAGEMVSHFGFEVPALVEPLDGGERGSGS
                     IEIRSEPFEPIASVRQQLLHAEMPIYEDGRLLGALVGHVIDEPDNLPFLPGVRPYLAA
                     LGPGAPFAPGELPYGGPAYVLYDAPGTVVITTIDRPPAMTEALVQALGSAGRVELSAG
                     GQRFSGLAVQDEFHRLHVLMIPIPGPLQRLASAARLIVLGVAWLALAALSTQLGRGRG
                     LADLVRAVRGSFYRKLLGALLVASMVPPIGLALLLRGYIDQRARDALDSAATRYAAAA
                     QRVLDDYAATLLETDVDYVSLLNDDIVDWLRNVIGQEIVVYENGVLQATSKRELFTSG
                     LLLDRLDGEVHRRLGREGLPFLVAQTALGPTRVPVAYAPVAGADPLRELVVAVPMITP
                     QLEVTRATDRVAEMILLSTVLLAGLLMFVAAAVARTVARPLRELVGATGRIASGDYAT
                     RLEARTRDEVADLVRGFNSMATSLATQRADLERRREYMERLLQHATTGVVSIAPDGTV
                     VTLNPAARELLASRWQRPRVGERLRDYLERFAEWGPLAQQLVAPAGSGEPVEVDVERQ
                     HEPRRLRMVRVDLPEPAGGTVGQLILLDDVTELMRSNQLAAWAEMARAIAHEIKNPLT
                     PIQLSTDHLARVLRDRGVLPDTAVEACIDTVIKQVRTLYEIAGEFSTYAKLPALAPRP
                     ADPLAFMRAAAAPYRAAPPDGLALEERYRPCGRAAIDERVLSRAVVNLIENALQAMPD
                     GGVLELGVGPGDTNGEIEISVADSGTGLSPEARKRLFEPYFSTKSSGTGLGLAIARKA
                     VEAHHGRIEVESRRPRGTRFRILLPRVDEPAEL"
BASE COUNT         1097 a         2509 c         2404 g          847 t
ORIGIN      
        1 gcgatccacc aggcgtcgag caggctgtcc cgtccgtccg cgatcaggtt gccccacgac
       61 gccatcggcg gtcgcacgcc cagctcgacg taggacagcg ccgactcgag caggatcgtg
      121 tcaccgaccc gcagcgtgcc ctcgacgacg accggaccga gcaccgccgg gagcagatgc
      181 cgcgtgccga gccggaacgg cgaagcaccg gccgcgcgcg cggcactgac gaactcgcgc
      241 tgtttgagcg acagcacctc gccgcgcacg agacgcgcgg cggccatcca ggtcgtcgta
      301 ccgagcacca agacggtggc cgacaacgac ggccggtaga gcgcgaccaa cagcagcgcg
      361 aggaacagcc gcggaacggc cagcaccacg tcggtcaagc gcatcagcag cgcgtcgacc
      421 cagccgccgg cgacccccgc cacgaggccg atgccggtgc cgaggccgag tgcgatcgcc
      481 gcggccgcca gaccgacgag cagcgagact cgggcgccgt ggaccaaccg gctcagcatg
      541 tcgcggccga agccgtccgt gccgagcggg taccacggcc gaacgttcca gtcggacgca
      601 agctccgcgc gagccagtac gatcgtcgtc cgcccgcggc gcacctcgac cgacccgtcg
      661 gccaacgggc gaaccgcctg ggcatagcgc agcccgccgt cggccagccg aactgcctgc
      721 gcgcgtgcca gcggcggcag atcgcgcagc accagcgtgt ccggttgggc gttcgggtca
      781 cgcagcccga gccacggtgc cgcgacgcag gtcaggccca gcacgacgaa ccaggcgacg
      841 gccagcttcg tcgtcccgct catggcgcgg cgcgtccgcc gccgagtcgc acgcgggggt
      901 cgagcgcggc cagcgcgagg tcggcgatca ggctaccgag cacgacgacg aaggccgaga
      961 tcgtcgtcgt cgccagcacg accggcaggt ccttggccat gatcgcgtcg taggtcaatc
     1021 ggcccattcc cggccaggcg aagacgatct cgatcacgag cgagcccgac accagcaccg
     1081 gcagcgacag gccgatcaga ttgaccaccg gtccgagcga cccgcgcagg gcatgcgcga
     1141 gcacgacccg ctggccgccg acaccgcgtg cgcgcgcggc gcggacgaac tcctgaccca
     1201 tcgactcgat cagaccggcc cgcacgaatc gcgtcatcgc cgcggccgac gagacggcga
     1261 gcacgcccgc gggcaaggcg aggtgccaga ggaggtcggc gaaacgcctc gccgcgcccc
     1321 actgctcggc gccgaccgac tgcatcgacg aggcgggaaa gagcggcacg aggaacgcga
     1381 gcccgaggat cgccatcaga ccgagccaga acggcggcat cgcgtagagc gtcaagctgc
     1441 acaacgtgac gatccggtcg ggccagcggc cgcgacgcgt cgccgagacg atgccgagcg
     1501 tcaccccgag caggacgtgg atcccgagcg cggccgtggt gagcaccagt gtcgccggca
     1561 gcgcgcgcgc cagcacctcc gccaccggcc gcgagcggga gatcgaccaa ccgagatcgc
     1621 cgcgcaccag cagcgcgccg acccaacggg cgtagcgttc gtgcgccggg cgatcgagac
     1681 cgtaggcccg ttcgacttgc cggcggactt cctgcggcag cggccggtcg ccgaggatca
     1741 ggtcggtcgg gcttccggga gcggtctcga gcagcgcgaa taccagcgtc gccacaccga
     1801 cgacgagcgg caccgacaac gcgaggcgcc tgaggacgaa ccacgccacg cgacgctcag
     1861 ggcgagtcga cccagaaccg ttcgagcgcg gccagcgggt cggccgggac gtcgacctcg
     1921 acaccgcgta gtcgcggacc gtgggccgcg atccgctgcg cctcgtacag cagcgtatag
     1981 ggctggtcgc ggtgcagctg gcgctggatc tcgtgcagcg gctggcccat cgcgatcgcg
     2041 ctgtcggccc gctgcaaccg gtcgagcagc tcgtcgagcg acgccgagcg gtactggacg
     2101 acgttcagcc cgttcggggg caggttgccc ccgccgaaca gcatcgccag gtcgacccgg
     2161 ccgacgaaga cccagccgcc gaggaaggcg tcgtagtcgc ccgcgccgac ctggtggcgc
     2221 agcgccttgg cctcgagcgg gtgcaccgtc gcttcgacgc cgacgagatc gagctgctgt
     2281 tggaccttga ccagcgcgtc gcggcgcagc cgattgccgt tgttcgtcag gatctcgatc
     2341 gtgagcacct cgcccggccg ctcgactcca tcggcgccga ccgtgcggaa cccgcggctc
     2401 gccaacaaac gacgcgcgcc ttccgggtcg tagggcagcg gacggatcga gcggtcggcg
     2461 ccccaccagc cggacggcac gggcgtaccc gcgacctcgc cgtagccgta gagcagctcc
     2521 tcgaccagcg ccgcgcgatc gatcgccatc gtcagcgcgc gccgcacttc cgcgtcgtcg
     2581 aacggcgcgc gcgcctcgtt ccaaccgatg aaatcgtagc cggggtagtc gaacgggacg
     2641 atcgacaccg cgctcgccgc gtcggccagc cgctcggctt cgcgcggcgg gattccgtcg
     2701 atccagtcga tctcgcccgc ctgcagctgg gtcaacagcg tcagcacgtc gggcacgatc
     2761 cggacgacga cgcggtccag ccgcggcagc tcgggttcga agtagtggag gttgcgctcc
     2821 agcacgatct cctgctgcgg gacgtgacgg ctgagcacga acggaccgga gccgatcgag
     2881 tactgcgacc agtcgtgcgt catccactcg tcgaacggga tctcgccgaa ccggtggcgc
     2941 ggcaggatgc cgccgtcgac ggcgtcggcg aactgatacg ggtagcggcc gtcgaagtgg
     3001 aagcggacgg tccgcgggtc gacgacctcg acatccgtga tgcgacgctt gttgaccgcg
     3061 ccgggccacg ggaccgccgg ctcggtctgc gcctgccagg tgaaccgcac gtcgtcggcg
     3121 gtcaacggaa cgccgtcgct ccagcgggcc tcgcgcagaa agaaggtgat cgccaggccg
     3181 tcctcggcga cctgccagtc ggtcgccagc gacggtgcga actcctccgg ccgctcgagg
     3241 ccggttccgc gatcgtccgc cagcctcaga tagatccgcc gcagcacgtt cgaggcgaac
     3301 gactgctgcg aggtgtactc gttccagctg tcgacgtccg agatgctccc cagcacggcg
     3361 gttccgccct gccgcgggct ctcgtcgtcc gcgccggcgc cgggccgctc caccggcggg
     3421 ccgccgcagg cgcacaccga caccagcgcg gccgacgcca acagcacccc gagcctcccg
     3481 ggccacgacc gacgccatcg atggatcatc acaccctccc cgcgcggcag gcgcacgggg
     3541 tcaaacttgg tggccccggc ccccggaggc aaggcgtcag tcgtcgggtg gccgtcgggc
     3601 caactgcccg cggatcgtca gcaggtcgtg gcgcggacct tggtcgatcc cggggcaaaa
     3661 gcgcttcagc cgctcccaac tgtcgcggct cgccagtgcc tcgtgccaga acgggaaggt
     3721 gctgcactgg gtcggtcgag cggcgtagac cccgcagcgg gtcccgtcga ggaacgggca
     3781 ggagggcgcg tccatccgca gcacgagctg gccgtcgtcc aggtcggtgt aacgcccgag
     3841 gaattcgtct tcgtcgatgc cgaggtgggc gacgaggcga tcgacgtcgt caccgacgag
     3901 gtagacgtag gcgtagtcgt cgtggttcgt acaacaggcc ccgcagttct ccttgcactc
     3961 gaaacggagc cccggggcgt accacgctgg gcgctcggcg tccattcgcg cgattatagc
     4021 tccgcgggtt cgtcgacgcg cggcagcagg atgcggaacc gggtgcctcg cgggcggcga
     4081 ctctcgacct cgatccgccc gtggtgcgcc tcgaccgcct tgcgcgcgat cgccaggccg
     4141 agcccggtac cggagctctt cgtcgaaaag tacggctcga acaaccgctt gcgcgcctcg
     4201 ggcgacaggc cggtcccgct gtcggccacg ctgatctcga tctcgccgtt ggtgtcgccc
     4261 ggcccgacac cgagctcgag cacgccgccg tcgggcatcg cttgcagcgc gttctcgatc
     4321 aggttgacca ccgcgcgcga cagcacccgc tcgtcgatcg ccgcgcggcc gcacgggcgg
     4381 tagcgttcct cgagcgccag cccgtcgggc ggagcggcac ggtagggcgc ggcggcggcc
     4441 cgcatgaacg ccagcggatc ggccgggcgc ggagcgagcg ccggcagttt ggcgtaggtc
     4501 gaaaactcac cggcgatctc gtacagtgtg cgcacctgct tgatcacggt gtcgatgcag
     4561 gcttcgaccg cggtgtcggg cagcacgccg cggtcgcgca gcacgcgggc caggtgatcg
     4621 gtcgacagct ggatcggcgt cagcggattc ttgatctcgt gcgcgatcgc gcgcgccatc
     4681 tcggcccagg ccgccagctg attgctgcgc atcagctcgg tgacgtcgtc caacaggatc
     4741 agctgcccga ccgttccgcc cgccggctcg ggcaggtcga cacgcaccat ccgcagccgc
     4801 cggggctcgt gctggcgctc gacgtcgacc tcgacgggct cgcccgagcc ggccggcgcg
     4861 acgagctgct gggccagcgg gccccactcg gcgaaccgct cgaggtagtc gcgcagccgc
     4921 tcgccgacgc gcggccgctg ccatcgactg gccaacagct cgcgcgccgc cgggttcagc
     4981 gtcaccaccg tgccgtccgg cgcgatcgag accacgccgg tcgtcgcgtg ttgcagcaac
     5041 cgttccatgt actcgcggcg gcgctcgagg tcggcgcgct gggtggcgag cgacgtcgcc
     5101 atgctgttga agccgcggac gaggtcggcc acctcgtcgc gggtccgcgc ctcgaggcgc
     5161 gtcgcgtaat cgcccgaggc gatccgaccc gtggcgccga ccagctcgcg cagcggacgc
     5221 gcgacggtgc gggccaccgc cgcggcgacg aacatcagca gtccggccaa cagcaccgtc
     5281 gacagcagga tcatctccgc cacgcgatcg gtggctcggg tcacttcgag ctggggagtg
     5341 atcatcggga cggcgaccac caactcgcgc aacgggtccg cgcccgcgac cggggcgtag
     5401 gcgacgggga cccgcgtcgg tccgagcgcc gtctgggcga cgagaaacgg caatccctcc
     5461 cggcccagac ggcggtggac ctcgccgtcg aggcggtcga gcagcaatcc ggaggtgaac
     5521 agctcccgtt tcgacgtcgc ctgcagcacg ccgttctcgt agacgacgat ctcctgcccg
     5581 atgacgttgc gcagccagtc gacgatgtcg tcgttgagca ggctgacgta gtcgacgtcg
     5641 gtctcgagca acgtggcggc gtagtcgtcc agcacccgtt gcgcggcggc ggcgtaacgc
     5701 gtcgcggcgc tgtcgagcgc atcgcgggcg cgctgatcga tgtagccgcg cagcagcagc
     5761 gcgagaccga tcggcggcac catcgaggcg acgagcagcg cgccgagcag cttgcggtag
     5821 aacgacccgc gcaccgcgcg cacgaggtcg gcgaggccgc ggccgcgccc gagctgggtc
     5881 gacagcgccg ccagcgcgag ccacgccacg ccgagcacga tcagccgcgc cgcggaggcc
     5941 agccgctgca gcggccccgg gatcgggatc atcagtacgt ggagacggtg aaactcgtcc
     6001 tgcacggcga gcccggagaa gcgctgcccg cccgcgctca gctcgactcg gcccgcgctc
     6061 ccgagggctt gcaccagcgc ttcggtcatc gccggcggcc ggtcgatcgt ggtgatcacg
     6121 accgttcccg gggcgtcgta cagcacgtac gccggtccgc cgtagggcag ctcgcccggc
     6181 gcgaacggcg ctcccggacc gagcgccgcg aggtacggtc tcacacccgg caggaacggg
     6241 agattgtccg gctcgtcgat gacgtgcccg accagcgcgc cgagcaaccg gccatcctcg
     6301 tagatcggca tctcggcgtg cagcagctgc tggcgcaccg acgcgatcgg ctcgaacggt
     6361 tcgctgcgta tctcgatcga cccgctgccg cgctcgcccc cgtcgagcgg ctcgaccagc
     6421 gcgggcacct cgaagccgaa gtggctgacc atttcgccgg cggggttgta gaacgacagc
     6481 gaggattggt agccgttgaa gaacagcggg ctcgagaccc agaactcgta ggccatcacg
     6541 tcgggccgag aactacgtgg gctgccgacc tcgtcgccgc gcccgaacgc ctcggcgacc
     6601 ccgcgcaccg cggcggtcaa cgcgacgctg cgccgtgccg actggtcgcg caccagcggc
     6661 acgaactccg agcgcagccg ttcgagcgcg agatcgcgtg tccggcgctc gagcgagaac
     6721 ccgccgccga ccaacagcag gctcgtcacc acgccgaccg cgacacgcga cgcacgacga
     6781 ccgtcgatcg gtcgagcgcg ccgcgcgatc gaccagccga cggcgacgag ctcggcgcag
     6841 ccgatcgcga cgacgat
//
LOCUS       DASPGN010000274        17002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_984336, whole genome shotgun sequence.
ACCESSION   DASPGN010000274 DASPGN010000000
VERSION     DASPGN010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 17002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17002
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984336"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VD788_07995"
     CDS             complement(<1..391)
                     /locus_tag="VD788_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV76246.1"
                     /translation="MLQTLRIRNLVIIEDLTLDFAPGLNLLTGETGTGKSIVVDAIGL
                     VAGRRADRSTVRSGAERAIVEALFRLDRDSPVGRWIDSSGQIDWPEDGELVVRREITA
                     TGGSVRVNGSPLAMSVLAELGERLLELH"
     gene            537..1229
                     /locus_tag="VD788_08000"
     CDS             537..1229
                     /locus_tag="VD788_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV76247.1"
                     /translation="MTAIPLGTAASGLDVWNLVMDSGPMAKLVLLCLLSFSVASWGII
                     AERFRRFRRAEDENRRFLARFRRGGGLAAIQDETKGLEWSPVAGIYRAGFREISLNPP
                     PDHEALRGDSLDAVDRVLRKNASVQFAEMERSLGFLATTASATPFIGLFGTVWGIMNA
                     FRGIGISGSASLAAYAPGIAEALVATAAGLGAAIPAVIGYNHFMGRLRQMDLVIDEFV
                     ADFIHRVQGRRS"
     gene            1233..1667
                     /locus_tag="VD788_08005"
     CDS             1233..1667
                     /locus_tag="VD788_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HXV76248.1"
                     /translation="MAAPIGAGGTGRGRRVLAEINVTPLVDVMLVLLIISMVAAPMLQ
                     RGINLELPATESASEISEARIEVSVDRDGRIRINDKPVHADLLHDRMEALAATAPDET
                     VFLRADKLLPYGEVLLVMDKIRTAGVTRISLVTVPLESAERR"
     gene            1664..2428
                     /locus_tag="VD788_08010"
     CDS             1664..2428
                     /locus_tag="VD788_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXV76249.1"
                     /translation="MSARRLRLRVVTSPLNSVQRFVAFSLLAHALLTAVLVWVPGIRR
                     RTRIPADATVVELVGALPAARPPAPAAPAPQPEPVAEPPASPPKKKPPDQASLAPEKT
                     EPRPEPVEPRPEPVEPPRDEAGSERPAEPVERPADQPTAGSSSEGASVSAIDLGDSAF
                     AWYRAAVANALYGNWRRPVLSGLREPVEVRVAFEIMRDGNVRGLRVELPSGVPMLDRS
                     ALRAVGDATPLPALPASLHEPYLPASIVFRLYPEGY"
     gene            2441..3742
                     /gene="tolB"
                     /locus_tag="VD788_08015"
     CDS             2441..3742
                     /gene="tolB"
                     /locus_tag="VD788_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HXV76250.1"
                     /translation="MIGRRIAFPAIALAFLLVPAGAGSAAQDRPRLDVELSAGMQPIR
                     IAVPAPLADRAEDENAAELVLTLRADLEFSGYFDVVDPSLYRRVPPPAGDDEPVEDWL
                     SIGAQAVARLRLRSSGERIDVEARLFDTEGGGLLFARRYGGSTELVRRVAHTVADDLI
                     EFYAGEGAGIATTRISFVSTHGDDKEVYLMDYDGSRIRRLTTTNTINLTPAWSPDGNE
                     LAYVSWRGRQPAVYVMSSDGKLGELGIVASELSSAPDWSPDGRRLVYSADVEGNVELF
                     VLDRLSGRNTRLTRHAAIETSPAYSPNGREIAFTSDRAGVPQIYLMDAEGVNVRRVSW
                     TGDYNDSAAWSPSGDRLAYASRVDGFFQIVVLDLSTEQVTPLTAARAHNENPRWSPDG
                     RHIVFASNRGGNYQIYTMRANGAEVRQLTRGRESVTPDWSR"
     gene            3860..4435
                     /gene="pal"
                     /locus_tag="VD788_08020"
     CDS             3860..4435
                     /gene="pal"
                     /locus_tag="VD788_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXV76251.1"
                     /translation="MQDKKKWALVICLVLSIVAFSAACKKKQPDQLTDPSATQPSTPT
                     PEPDERAEPPVQVEEPFEQQEPDTEDLDATAQALNAQEVLKTVYFAFDRAELTDTARE
                     ILRENANWLKANPKWNVAIEGHCDERGTIEYNLALGQRRANTVRDYLTSLGVPASRIR
                     SVSYGEERPADPGHTEASWAKNRRGVSLVEN"
     gene            4444..5280
                     /gene="ybgF"
                     /locus_tag="VD788_08025"
     CDS             4444..5280
                     /gene="ybgF"
                     /locus_tag="VD788_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HXV76252.1"
                     /translation="MEAKTIWGALGLVAVTAASSGCVMPDKVAGVQKNLADIQADIRQ
                     IQQSQDETRARLETLEQRTANDEDRVTKADFADVTVSIDQLSRDVAIVDERVNDISRR
                     FDRMSQQVQQARESRSIPYPGGEPSDAGMEPVIVDDGSARLPSTGAGAVPDPDALYNT
                     AYADFSKGNYALAVSGFEEYHEKFPASALADNALYWVGECHYSQGNFPDAIRAFDQML
                     ETYPQSDKAAASNLKKGLAYLEQNEVGQAIVQLRYVVAEYPGSDEAKIARDKLTSLGA
                     PS"
     gene            5379..5651
                     /gene="rpsT"
                     /locus_tag="VD788_08030"
     CDS             5379..5651
                     /gene="rpsT"
                     /locus_tag="VD788_08030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXV76253.1"
                     /translation="MATHKSAEKEHRRSLARRERNRHKRARLRTAIKKFRAAVAEGDL
                     EKARGMLPGTVALLDRTAKVRAIHPGAADRAKSRLTRLLNRTAAAG"
     gene            complement(5660..6088)
                     /locus_tag="VD788_08035"
     CDS             complement(5660..6088)
                     /locus_tag="VD788_08035"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HXV76254.1"
                     /translation="MDGLRRILARPWLVRAAQVGIGVVLAWAGLAKIVDQAGFAAQVH
                     NFKLVPIAVENLIAMTLPWVELVTAHALIFGVRARAGAVLASGLVGVFTLAVLAALAR
                     GMDIECGCFGTGDASRVGWIKLGQNLAMLAVAVVGTLRPR"
     gene            complement(6088..6738)
                     /locus_tag="VD788_08040"
     CDS             complement(6088..6738)
                     /locus_tag="VD788_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXV76255.1"
                     /translation="MPQPSPTTDRERLARSIVILCATGVALGVSFNYFALQQPRGRGL
                     PWIAEDRMAALASGPSVRADGGNGSPSASAERDEFTTANADPFAVSSDTPALPEIPAI
                     GRPVAIETEAVELYVEAGAALILDAREADEYHAGHIPGAVNLPYERVITDPAALETLE
                     TDGRPIIAYCGGGECEVSLSLANELIALGFERVAVYTGGFPEWEAFELPVARGDGR"
     gene            complement(6838..7341)
                     /locus_tag="VD788_08045"
     CDS             complement(6838..7341)
                     /locus_tag="VD788_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXV76256.1"
                     /translation="MPTTARDIMNKEVVSVPTTMDLRDLARMFLEKGITGAPVVDDNG
                     DLAGVVSQTDLIYYGLTRDDELVLDSMFYQTARMEGRHVPPGFQIEDTNTGVVADVMT
                     PLVHSVTERASVQSVARLMTDKHIHRVIVRKGRKVAGIISALDVLRAEVDGRRSLRGA
                     PAGRAAR"
     gene            complement(7435..9540)
                     /gene="rnr"
                     /locus_tag="VD788_08050"
     CDS             complement(7435..9540)
                     /gene="rnr"
                     /locus_tag="VD788_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HXV76257.1"
                     /translation="MPVDDEPREALLEYLSRPDYRPLTQKQLLHRMRVAGQAREHVRR
                     AIRGLQDEGRLASGRGGRLMLRAPERVSGVLHRHRDGYGFVVPDEHGLDVFVAPPNLG
                     DHLSGDRVEARITRRGRGNRLEGVIVRTIERRSRRSLGVFFGSPPGGSVQPFEAGLSD
                     PVHVPEKFRSGARPRQVVRFEIVHGPGRGQPAEGKVLEVLGELDEPGMDVRIVARKYG
                     LEQRFSPVVERAAAKLPSRIGKQRAAGRTRFSDPPPITIDGETAQDFDDAVAVERTRD
                     GFRLYVHIADVASFVEANDPIDEEARRRGTSVYFPGTVLPMLPEELSNELCSLRPGVD
                     RLVQTAIVDFDPDGEPVRTEFADGIIRSAARLTYEQVERVIAGERVAGVPRVVLPMIG
                     AADRLRRLHERRRQRRGSIDFDLPEPEILLDVEGEMTGVRVEPRNRAHRLIEEFMIAT
                     NEAVARYLESGVDACVFRVHDRPDPAKLEGLARFVSRFGLDFPTDGQTIESRDYQRLL
                     AAVDAKPESSLVNSVALRSMKQARYSTDNIGHFSLASSCYCHFTSPIRRYPDLLIHRL
                     LRAARSGRAPGETERADLELWAERCSELERNAESAERELLNWKKVAYIEDKVGESFDS
                     VVTGVTTFGLFCRLESNLVEGLLRVEELGDERFDFDASRQELLGRRSGRAWGLGQRLR
                     VRVERVDRVLQRVDFAPAD"
     gene            9623..9709
                     /locus_tag="VD788_08055"
     tRNA            9623..9709
                     /locus_tag="VD788_08055"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9657..9659,aa:Leu,seq:cag)
     gene            complement(9721..9894)
                     /locus_tag="VD788_08060"
     CDS             complement(9721..9894)
                     /locus_tag="VD788_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76258.1"
                     /translation="MRDRRTDRTGTIVEIARQYSHPKADPVFNYLIRWEDGQIQAVSE
                     QAFSRGYDLEPAD"
     gene            10328..10747
                     /locus_tag="VD788_08065"
     CDS             10328..10747
                     /locus_tag="VD788_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76259.1"
                     /translation="MEHGPKSIGDQIHCLARLTGAPESFVYQVRALFTRKGISLEDDA
                     APYRRALEEAFVREECIRIGAEAPAAVSKVVSDDDPAASYRREVNRLRRAQAQLRGRR
                     RRAIPGAAVAFGTSGRSLVTRQQREDLPMVPGPEEPQ"
     gene            complement(10749..12683)
                     /locus_tag="VD788_08070"
     CDS             complement(10749..12683)
                     /locus_tag="VD788_08070"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HXV76260.1"
                     /translation="MVRRDGSRPYATGLGCASAVVLLYVWTACPTVYYGDGGELIAAA
                     DSLGVAHPPGYPLYTALAKVALAVPLGEAAWRVNLMSSVFGALAAGLVAVLVARWTGS
                     ALAALAAGAALAVTRDVWAIATVAEVYTLHLFLVTALLVVADEWHGAVDRRARRRWTW
                     VAAALVGVGLAHRPTVLLALPALVVLAWDGGPRQPIGRRCVAGATGLAAAIPVAAYTW
                     LMWRSSLEPAVNWAAPDHPLALWNHVTTRGYRLYVLGPAGWLELAGWRRLIAMLWSGA
                     GYVGLPLAIAGLAARFASRGSRDRPALAGLTLGGAWAGFGLMYGTEDVEVLFLPCAVA
                     AALGAGLGLPLLSGAIPRALRPVPAIVLVLAPWALNHPSADLRRATGARDYGRDVLAT
                     VPPAGVLFVEGDNAFVLAYLQQVLGERRDVTIYDRNGLMFHDLLDEPGSPPGPDESPL
                     GFRVRREIEYLTAAVGERPVQFLVWPGYELPRGFRFEPEGLSYRVLPPGHPRLDTAGT
                     WGGYHEESVIAEARRTDNPFARTVAASYPLMRGERARFDGDRPAALRWFERATDLARS
                     ETIHNYLGTVFGRLGELERAKREFRRAIAAKPVSVRAWNNLGRAESLSGNPAAARQAW
                     RRSLAIEPDQPETRRLLGSD"
     gene            complement(12692..13885)
                     /locus_tag="VD788_08075"
     CDS             complement(12692..13885)
                     /locus_tag="VD788_08075"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXV76261.1"
                     /translation="MVSRRAASIGSSPTLRISALAQAMRAEGIDVLDFSAGQPDFPVP
                     AAAKRAASEALAQDRTRYTPNAGLPELRSAIAGSLARTRGLDYPPGQILVSTGAKASL
                     YFAFLALVDAGDEVLVPAPYWVSYPEQVALAGGTPVIVPCPEQGGFKLTVDALERAAS
                     PRTRALVLNYPSNPTGACYRRDELDAIARFCVERGIWVLADEIYSRLVYDGLPFTSIA
                     ELSPEIRERTVVIDGMSKSYSMTGLRIGYAAGPEEVIAAMSRLQSHSTSNACSISQWA
                     SIEALASCEDEVSRRVAEFERRRDEIVRLLGEVDGVRCLVPDGAFYVFPSVDGLIGRR
                     AGDRRIDGGDRFAEYLLAQARVAVVPGTAFGAPDHVRISYAVSVDRIREGVRRIAEAV
                     AALAE"
     gene            complement(13912..14604)
                     /locus_tag="VD788_08080"
     CDS             complement(13912..14604)
                     /locus_tag="VD788_08080"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV76262.1"
                     /translation="MKFPWSRAGSSTGLSGQPDAAAGGEAVQKCPGLGKVLEKIGRID
                     KPEVLDLGPFCGDTAVYFGERGARISVAELEPPPPPPPPAERDPDDLAARTSEPPAVP
                     PIRLAFDDSRFDLVLVWEMCDFLPPDRVGEFGAELHRVMADGGTALLMSASDPPKDGP
                     HADRAPRYRVVDEGHVERQAGSGPRRRRYGHNPRALERALAPLGVASIHLQRNQLREF
                     IFQKKRAVGPKR"
     gene            complement(14608..15048)
                     /locus_tag="VD788_08085"
     CDS             complement(14608..15048)
                     /locus_tag="VD788_08085"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXV76263.1"
                     /translation="MESIVNIGQSVEIKGTLTGNEDLTIEGLVDGKILVKDHSLTIGA
                     NGRITAEVHAKNVVVVGQVVGNITADDKVEVAPSGSVHGDVRAPRVAIADGAKFKGSI
                     DMDRKTGVPATASPSAHASTATKPVGSGVLPPGSITRSAGSTKG"
     gene            15318..16823
                     /locus_tag="VD788_08090"
     CDS             15318..16823
                     /locus_tag="VD788_08090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HXV76264.1"
                     /translation="MPPQLRLLLVTLPLAVGLADASPTAAASNELKQWIDGPVRYISR
                     KQEAKLFHELETDEARAAFIARFWRRRDPDPETLTNEYRELFWRRVKEANQSFLDSSK
                     QGWMTDRGKIWILYGPPTDVEDYTDLQANDAAGHGVIRWIYEGRPGERIDMRPIVVVP
                     FERQTDGEYRVSYDPQLASVFFDATGIAEGQNDIYDKFFELAGAPRQSELSVMLDLGR
                     MQEVPPAEQVLIESVETMESYGTLELPVAVNHYFRPEDGRPLAVINVELTDLGQIDDA
                     AILARFRPVEPTRETRVLGEDSFRLATAGDYRLAQGRLSLEPGRYDVTVVVADPIHVQ
                     TALYKAELDVPETPRTLSLSQPVWAAEMAQVEYRSLASYDEPFHLGPFRVLPLLTATY
                     HRGDSAKLFVETYGAEYPLTATHQLEGLEADGSWVALGQPAVIEQSAAGLAWEVQTGP
                     RWPLGRYRVRVEVVDAAGRTARTEAEFALDEAELDGPAADTDDGAAGDSDS"
BASE COUNT         2655 a         5997 c         5869 g         2481 t
ORIGIN      
        1 cgtgcagctc gagcagccgt tcaccgagct cggccagcac cgacatcgcg agcggtgagc
       61 cgttgacgcg gacgctgccg cccgtcgcgg tgatctcgcg gcgcacgacc agctcgccgt
      121 cctccggcca gtcgatctgc ccactcgagt cgatccagcg tccgacgggc gaatcgcggt
      181 cgagacgaaa cagcgcttcg acgatcgcgc gctccgcacc gctgcggacc gtcgaacggt
      241 cggcgcgccg gcccgcgacg agaccgatcg cgtcgaccac gatcgacttg ccggtcccgg
      301 tctcgcccgt cagcaggttg agccccgggg cgaagtcgag cgtgaggtcc tcgatgatga
      361 cgaggtttcg gatcctgagg gtttgcagca tttgcaactt gggccggcac gcggcgccgc
      421 gcatgcgacc ggggcgtcac gctttgacgc tccccgagcc ctgggatagc atacctggcg
      481 tttgccgtcg gcgtgaaaga ttcgattgtc gggcgttcgc tgctgttgga gtgcaagtga
      541 ccgcgatccc cctcggcacg gcagcctccg gactcgacgt gtggaacctg gtcatggact
      601 ccggtccgat ggccaagctc gtgctgctct gtctgctctc cttctcggtc gcgtcgtggg
      661 gtatcatcgc cgagcggttc cgccggttcc ggcgcgccga ggacgagaac cggcgctttc
      721 ttgcccgttt tcgccggggt ggcggtctcg ccgcgatcca ggacgagacg aagggtctcg
      781 aatggagccc ggtcgccggc atctatcgcg ccggcttccg cgagatcagc ctgaaccccc
      841 cgcccgatca cgaagcgctg cgcggcgact cgctcgacgc tgtcgatcgc gtgctgcgca
      901 agaacgcctc ggtgcagttt gccgagatgg aacgcagtct cggcttcctc gccaccaccg
      961 ccagtgcgac gccgttcatc ggcctgttcg gcacggtgtg gggcatcatg aacgccttcc
     1021 gcgggatcgg catcagcggc agcgccagcc tggcggccta cgcaccgggc atcgccgagg
     1081 cgttggtcgc gacggcggcc gggctcggcg cggcgatccc ggcggtcatc ggctacaacc
     1141 acttcatggg ccggttgcgt cagatggacc tcgtcatcga cgagttcgtc gccgacttca
     1201 tccaccgcgt ccaggggcgg aggagttgac gaatggccgc gccgatcggt gcgggcggga
     1261 ccggacgcgg ccgccgcgtg ctggccgaga tcaacgtcac gccgctggtc gacgtgatgc
     1321 tcgtgctgtt gatcatctcg atggtcgcgg cgccgatgct gcaacgcggg atcaacctcg
     1381 agcttcccgc gaccgagagc gccagcgaga tcagcgaggc gcgcatcgaa gtctccgtcg
     1441 atcgtgacgg tcggatccgc atcaacgaca agccggtcca cgccgacctg ctgcacgatc
     1501 ggatggaggc gctggccgcc accgccccgg acgagacggt cttcctgcgc gccgacaagc
     1561 tgctgcccta cggcgaggtg ctgttggtaa tggacaagat ccgcacggcc ggagtgactc
     1621 ggatctcgct cgtgacggta ccgctcgaat cggcggagcg acgttgagcg cgcggcgctt
     1681 gcgcctgcgc gtcgtcacct ccccgctgaa ctcggtgcag cggttcgtcg ccttctcgtt
     1741 gctggcgcac gcgctgctga ccgccgtgct ggtgtgggtg cccgggatcc ggcggcgtac
     1801 gaggattccg gccgacgcga cggtcgtcga gctcgtcggt gccctgccgg cagcgcggcc
     1861 accggcgccg gcggcacccg ccccgcagcc ggaaccggtc gccgaaccgc ccgcctcacc
     1921 gccgaagaag aagccccccg accaggcgag cctcgcgccc gagaaaaccg agccacgccc
     1981 ggagccggtc gaaccccgac cggaaccggt cgagcccccg cgggacgagg caggatccga
     2041 gagaccggcg gagccggtcg aacggcctgc cgaccagccg acggccgggt cgtcgtccga
     2101 gggggcgtcg gtctccgcca tcgacctcgg cgacagcgcc ttcgcctggt accgcgccgc
     2161 ggtcgccaac gcgctgtacg gcaactggcg ccggccggta ctgagcgggt tgcgcgagcc
     2221 ggtcgaggtt cgcgtcgcgt tcgagatcat gcgcgacggc aacgtgcggg gcctgcgcgt
     2281 cgagctgccg agcggcgtgc cgatgctcga tcgatcggcg ctgcgcgcgg tcggcgacgc
     2341 gacgccgctg ccggcactgc cggccagttt gcacgagccg tatctgccgg ccagcatcgt
     2401 ttttcgtttg taccccgaag gctactgaac aggagcttcc atgatcggtc gtcgaatcgc
     2461 cttcccggcc atcgctctgg ccttcctgct cgtgccggcc ggcgcgggct ccgcggcgca
     2521 ggaccgacct cgcctcgacg tcgagctgtc ggccggcatg cagccgatcc ggatcgctgt
     2581 acctgcgccg ctggccgatc gtgccgagga cgagaacgcg gcggagctgg tgctcacgct
     2641 gcgcgccgat ctcgagttca gcggttactt cgacgtcgtc gacccgtcgc tctatcggcg
     2701 cgtcccccca cccgcgggcg acgacgagcc ggtcgaggat tggctgtcga tcggcgcgca
     2761 ggcggtggca cgcctgcggc tgcgctcgtc gggcgagcgg atcgacgtcg aggcgcgcct
     2821 gttcgacacc gaaggaggcg gactgctgtt cgcccgacgc tacggcggct cgaccgagct
     2881 ggtacggcgg gtggcgcaca cggtcgccga cgacctgatc gagttctacg ccggcgaggg
     2941 cgccggcatc gcgacgacgc ggatcagctt cgtgtcgacc cacggcgacg acaaggaagt
     3001 ctatctgatg gactacgacg ggagtcggat ccgccggttg acgacgacca acacgatcaa
     3061 cctcacgccg gcctggtcgc ccgacggcaa cgaactggcc tacgtctcgt ggcgcggccg
     3121 gcagccggcg gtctacgtga tgagcagcga cggcaagttg ggcgagctcg gtatcgtcgc
     3181 cagcgagctc agctcggcgc ccgactggtc gcccgacggg cggcggctgg tctacagcgc
     3241 cgacgtcgag ggcaatgtcg agctgttcgt gctcgatcgc ctctccgggc gcaatacccg
     3301 gctgacgcgg cacgccgcga tcgagacctc gccggcctac tcgccgaacg gtcgcgaaat
     3361 cgcgttcacc tcggaccgcg ccggcgtgcc gcagatctac ctgatggacg ccgagggggt
     3421 caacgtgcgg cgggtgtcgt ggaccggcga ctacaacgat tccgccgcct ggtccccgag
     3481 cggggaccgg ctggcctacg cctcgcgagt cgacggtttc ttccagatcg tcgtgctcga
     3541 cctgtcgacg gaacaggtca cgccattgac cgctgcccgg gcccacaacg agaacccgcg
     3601 ctggtcgccg gacggccgcc acatcgtctt cgcgtcgaac cgcggcggca actaccagat
     3661 ctacaccatg cgggcgaacg gtgcggaggt gaggcagctc acgcgcgggc gcgagtcggt
     3721 cacgcccgac tggtcgcgct gatccggaat cgcgggagtc ggttgatctg gcggtgcggc
     3781 tcgatataat cgtcccgacc gcgtcgcgta tgacggccgt ccgaatgtcg gtggccgagc
     3841 gaggtgagga agccgaacga tgcaagacaa gaagaagtgg gccctggtca tctgtttggt
     3901 gctatcgatc gtcgcgttct cggccgcttg caagaaaaag cagccggacc agctgaccga
     3961 tccgtcggca acgcagccca gcacaccgac acccgagccc gacgagcgcg ccgagccacc
     4021 ggttcaggtc gaagagccgt tcgagcaaca ggagcccgac accgaagacc tcgatgcgac
     4081 cgcccaggcg ctcaacgccc aggaggtgct gaagacggtc tatttcgcct tcgatcgtgc
     4141 cgagctgacc gacaccgcgc gtgagatcct ccgcgagaac gcgaactggc tcaaggccaa
     4201 tccgaagtgg aacgtcgcga tcgaaggcca ctgcgacgag cgcggcacga tcgagtacaa
     4261 cctggccctc ggccagcgac gtgccaacac cgttcgcgac tacctgacca gtctgggcgt
     4321 gcccgcctcc cgcatccgct ccgtctccta cggcgaggag cggccggccg atccgggcca
     4381 caccgaagcg tcgtgggcca agaaccgccg cggcgtgtcg ttggtcgaaa actaggagcg
     4441 acgatggaag ccaagacgat ctggggcgcg ctcgggctgg tcgcggttac ggccgcctcg
     4501 tccggctgcg tgatgccgga caaggtcgcc ggggtgcaga agaacctcgc cgacatccag
     4561 gccgacatcc gccagatcca gcagagccag gacgagaccc gcgctcggct ggagacgctc
     4621 gaacaacgca cggccaacga cgaagaccgg gtgaccaagg ccgacttcgc cgacgtgacg
     4681 gtgagcatcg accagctgtc gcgcgatgtc gcgatcgtcg acgagcgggt gaacgacatc
     4741 agccggcgct tcgaccggat gtcgcagcaa gtgcagcagg cccgcgagtc acggtcgatc
     4801 ccctatcccg gcggggaacc gtcggatgcg gggatggaac cggtgatcgt cgacgacggc
     4861 tccgcccggc tgccgtcgac gggcgccggc gccgtccccg accccgacgc gctgtacaac
     4921 acggcatacg ccgatttctc gaagggcaac tacgcgctgg cggtctcggg cttcgaggaa
     4981 taccacgaaa agtttccggc cagcgcgctg gccgacaacg cgctgtactg ggtcggggag
     5041 tgccattaca gccaaggcaa cttccccgac gcgatccgcg ccttcgatca gatgctcgag
     5101 acctatccgc agagtgacaa ggccgccgcg agcaacctca agaagggact ggcctacctc
     5161 gagcagaacg aggtcgggca ggcgatcgtc cagctgcgct acgtcgtcgc cgagtacccg
     5221 ggcagcgacg aggcgaagat cgcgcgcgac aagctgacca gcctcggggc gccgtcgtga
     5281 gcgacggccg gtccggttga cgggaccggg ccgctgaact agaatccgcg ctttccgggc
     5341 gggcgtcgga tccggccggg acgacgggag tgatccgcat ggccacccac aagagcgccg
     5401 agaaagagca ccgccggagc ctggcacggc gcgagcggaa ccgccacaag cgcgcgcggc
     5461 tccgcaccgc gatcaagaag ttccgcgcgg cggtggcgga aggcgatctg gaaaaggcgc
     5521 gcgggatgct cccgggaacc gtcgcgttgc tcgaccggac ggcgaaggta cgcgcgatcc
     5581 acccgggggc ggccgatcgc gccaagtccc gcctgacccg cctgctcaac cggaccgcgg
     5641 ccgccggctg agcggcacgc tagcggggac ggagcgtgcc gacgaccgcg accgccagca
     5701 tcgccaggtt ctgacccagc ttgatccagc cgacgcgcga agcgtcgccg gtgccgaagc
     5761 agccgcactc gatgtccatc ccgcgggcga gcgcggccag cacggccagc gtgaagactc
     5821 cgaccagtcc gctggccaac acggcccccg cacgtgcacg gacgccgaag atcagcgcgt
     5881 gcgccgtcac cagctcgacc catggcaggg tcatcgcgat cagattctcg accgcgatcg
     5941 gaaccagctt gaaattgtgg acctgggcgg cgaaaccggc ctggtcgacg atcttcgcca
     6001 gaccggccca cgccagcacc acgccgatcc cgacttgcgc cgcgcgcacg agccacggac
     6061 gggcgagaat gcgtcgcagc ccgtccatca gcggccgtcc ccgcgcgcga cgggaagctc
     6121 gaacgcctcc cactcgggga acccgcccgt atagaccgcg acgcgctcga aaccgagcgc
     6181 gatcagctcg ttggcgaggc tgagcgacac ctcgcactcg ccgccgccac agtacgcgat
     6241 gatcggccgc ccgtcggtct cgagcgtctc gagcgcggcc gggtcggtga tcacgcgttc
     6301 gtagggcaag ttgaccgcgc ccgggatgtg tccggcgtgg tactcgtcgg cctcacgggc
     6361 atcgaggatc agcgccgccc cggcctcgac gtacaactcg acggcttcgg tctcgatcgc
     6421 caccggccgg ccgatcgccg ggatctcggg cagcgccggc gtgtcgctcg agacggcgaa
     6481 cgggtcggcg ttcgccgtgg tgaactcgtc cctctcggcg gacgcgctcg gcgacccgtt
     6541 tccgccgtcg gcccgaacgc tagggccgct ggccagcgcc gccatccggt cctccgcgat
     6601 ccacggcagg ccccgaccgc gcggctgctg cagcgcgaag taattgaacg agacgccgag
     6661 tgccaccccg gtcgcgcaga ggatcacgat gctgcgcgcg aggcgctcgc gatcggtcgt
     6721 cgggcttggc tgcggcatcg agcgtctccg tgtcgcgggc gagccggctc acccgaccca
     6781 gtatccgcgc gggaggtgtc gggcggcaac gccggccccg gtcattttac ccggctttta
     6841 ccgagccgcg cgacccgccg gcgcgccgcg cagcgaacgt cttccgtcga cctcggcgcg
     6901 cagcacgtcg agcgccgaga tgatgccggc gaccttgcga cccttgcgca cgatgacccg
     6961 gtggatgtgc ttgtcggtca tcagtcgagc caccgactgg acactcgcgc gctcggtcac
     7021 cgagtggacg agcggcgtca tcacgtcggc gacgacgccg gtgttcgtat cttcgatctg
     7081 gaagccgggc ggtacgtggc gaccctccat gcgagcggtc tggtagaaca tcgaatcgag
     7141 caccagctcg tcatcgcgcg tcaggccgta gtagatcaga tcggtttgag aaacgacgcc
     7201 ggccaggtcg ccgttgtcgt cgacgaccgg cgccccggtg atccccttct cgaggaacat
     7261 cctggcgagg tctcgcaggt ccatcgtggt cgggaccgag accacttctt tgttcatgat
     7321 gtctcgcgcc gtcgtcggca tgacgacctc cttgaccgag tcgttcggag gacaccggca
     7381 ttgtacaaca gcggccccgc tctcggcgca gcgggtggcg cgccccggat ccgctcagtc
     7441 ggccggagcg aaatcgacgc gctgcagcac gcggtcgact cgctcgacgc ggacgcgcag
     7501 acgttggccc agaccccagg cgcggccact gcgacgcccg agcagctcct gtcgcgacgc
     7561 gtcgaagtcg aaacgctcgt cgcccagctc ctcgacgcgc aacaggccct cgaccaggtt
     7621 cgactcgaga cggcagaaca acccgaacgt cgtcacgccc gtcacgaccg agtcgaacga
     7681 ttctcccacc ttgtcctcga tgtacgccac cttcttccag ttgagcagct cgcgctccgc
     7741 cgactccgcg ttgcgttcga gctcggagca acgctcggcc cacagctcga ggtcggcgcg
     7801 ttccgtctcg cccggcgcac gccccgagcg cgcggcgcgc aacaggcggt ggatcaacag
     7861 atcgggatag cgccggatcg gcgaggtgaa gtgacagtag caggacgagg ccaggctgaa
     7921 gtggccgatg ttgtccgtcg agtagcgcgc ctgcttcatc gagcgcagcg ccaccgagtt
     7981 gaccagcgac gactccggtt tggcgtcgac cgcggcgagc agtcgctggt agtcgcgcga
     8041 ttcgatcgtt tgcccgtcgg tcgggaagtc gagaccgaag cgggagacga accgggccaa
     8101 tccctcgagc tttgccgggt ccggtctgtc gtgcacacga aagacgcacg cgtcgacccc
     8161 gctctcgaga tagcgggcga cggcttcgtt ggtcgcgatc atgaactctt cgatcaagcg
     8221 gtgcgcgcgg ttgcgcggct cgacgcgcac cccggtcatc tcgccctcga cgtcgagcag
     8281 gatctcgggc tccggtagat cgaagtcgat gctgccgcga cgctgacgcc gccgctcgtg
     8341 gagccggcgc agccggtcgg cggcgccgat catcggcagc acgacgcgcg gcacgccggc
     8401 cacacgctct ccggcgatga cgcgctcgac ttgctcgtag gtcaggcgcg cggccgaacg
     8461 gatgatgccg tcggcgaact cggtccggac cggctccccg tcgggatcga agtcgacgat
     8521 cgccgtctgc acgaggcggt cgactcccgg acgtagcgag cacagctcgt tcgacagctc
     8581 ttcgggaagc atcggcagca ccgtgccggg aaagtagacg ctcgtgccgc gccgccgggc
     8641 ctcctcgtcg atcgggtcgt tggcctcgac gaaagacgcg acgtcggcga tgtggacgta
     8701 cagccggaac ccgtcgcgcg tgcgctcgac ggccacggcg tcgtcgaagt cctgggccgt
     8761 ctcgccgtcg atcgtgatcg gcggcggatc ggagaagcgg gtccggccgg cggcgcgctg
     8821 cttgccgatc cgcgacggca gcttcgccgc ggcgcgctcg acgaccggcg agaaccgctg
     8881 ttcaagaccg tacttgcgcg cgacgatccg cacgtccatc cccggctcgt ccagctcgcc
     8941 gagcacctcg agcaccttgc cttcggcggg ctggccgcgc cccggcccgt ggacgatctc
     9001 gaagcgcacg acctggcgcg gccgggcacc cgagcggaac ttctcgggca cgtgcaccgg
     9061 atcggagagg ccggcctcga acggctggac gctgccgccg ggcgggctgc cgaagaagac
     9121 gccgaggctg cggcggctgc gccgctcgat cgtgcggacg atcacgccct cgagccggtt
     9181 gccgcgaccc cggcgcgtga tccgcgcttc gacgcgatcg cccgacaggt ggtcgcccag
     9241 gttcggcggc gcgacgaaga cgtcgagccc gtgctcgtcg gggacgacga agccgtagcc
     9301 gtcgcgatga cggtgcagca cgccgctcac acgctccggc gcgcgcagca tcaaccttcc
     9361 gccccgtccg ctggccaagc gcccctcgtc ctgcagaccg cggatcgcac ggcgcacgtg
     9421 ctcgcgcgct tggcccgcga cgcgcatgcg gtgcagcagc tgcttctgcg tcagcgggcg
     9481 gtagtcgggg cgcgacaggt actcgagtag cgcctcgcgc ggttcgtcat cgacgggcat
     9541 gcggtgttgc gttcgaggtt tgcagcggac ggcccttatc ttgacactgt ttcggtcgat
     9601 tgctaggttg gatcgtcccc tcgccgaagt ggcggaattg gtagacgcgc tagattcagg
     9661 atctagtggg cgcgagcctg tgggagttcg aatctcccct tcggcaccaa cgagttcgtc
     9721 tcagtcggcg ggctcgaggt cgtagccgcg cgagaacgcc tgctccgaga cggcctgaat
     9781 ctgaccgtcc tcccagcgta tcagatagtt gaagaccggg tcggccttgg gatgcgagta
     9841 ttggcgggcg atctcgacga tggtgccggt ccgatccgtc cgtcgatccc gcacgcgccg
     9901 accgatctcg agcggctgat gggaacgggt ggctccgcgg ccgccccgcg gccctctcgg
     9961 ttgtcggtta cggttcaact ccacgactcc gccccccaag ccgagcgcac aaacacccgg
    10021 cccgctcccg aacctcaccg tctcgcgtcg tgtggtgttc cgctatcttc tccgaatact
    10081 aacccaagac aggctagctt ggtggttttt cgggcgccag gacctttcgc gccatccgac
    10141 ggcgcggact gccggccggc cggcggagga atgcgagagt tcggcgttgt cgcgcttgtc
    10201 gcggtttcct ggtcgaaccg gcccgtcggg cggaccgatg gcttgatccg tcaggcgccg
    10261 gagagttctt gactccccga cttttgtccg tatcaagttc ggtgagcgac gtatggagag
    10321 acgcgccatg gaacacggac cgaagtcgat cggagaccag atccactgtc tcgcccgcct
    10381 gaccggtgct ccggaatcgt tcgtctacca ggtccgcgcg ctgttcaccc gcaagggcat
    10441 ctcgctcgaa gacgacgcgg cgccctaccg gcgggcgctc gaagaggcgt tcgtgcgcga
    10501 ggagtgcatc cgcatcggcg cggaggcgcc ggccgcggtc tcgaaggtcg tctcggacga
    10561 cgacccggcc gcgtcctacc gtcgcgaggt caaccgtcta cgtcgggccc aggcccaact
    10621 gcgcggccga cgccgtcgag cgatacccgg cgcggcggtt gccttcggca cctcgggccg
    10681 ttcgctcgtg acccgccagc aacgcgagga cctgccgatg gtccccggtc cggaagaacc
    10741 ccagtaggtc agtcggaacc gagcaggcgc cgcgtctccg gctggtccgg ctcgatcgcg
    10801 agcgatctgc gccacgcttg ccgcgccgcc gcagggttgc ccgacagcga ctcggcgcgg
    10861 ccgaggttgt tccaggcccg gaccgagacg ggcttggccg cgatcgcgcg ccggaactcg
    10921 cgcttggcgc gctcgagctc ccccagccgg ccgaagacgg tgccgaggta gttgtggatc
    10981 gtctcgcttc gggcgaggtc ggtggcgcgc tcgaaccagc gcaacgcagc ggggcgatcg
    11041 ccgtcgaacc gtgcccgctc gccgcgcatc aacggatagc tcgcggcgac cgtccgtgcg
    11101 aacgggttgt cggtgcgtcg ggcctccgcg atcaccgatt cctcgtggta gccgccccac
    11161 gtcccggcgg tatcgagccg ggggtggccc ggcggaagga cccgatacga cagaccctcc
    11221 ggctcgaagc gaaagccgcg cggcagctcg tagccaggcc agaccaggaa ctgtaccggg
    11281 cgctcgccga ccgccgcggt caggtactcg atctcgcggc gcacgcggaa cccgagtggg
    11341 ctctcgtcgg gccccggcgg cgatccgggc tcgtcgagca ggtcgtggaa catcagcccg
    11401 ttgcggtcat agatcgtgac gtcgcgtcgt tcgccgagca cttgctggag gtaggccagg
    11461 acgaacgcgt tgtccccctc gacgaacagc acccccgcgg gtggaaccgt cgccaggacg
    11521 tccctgccgt agtcgcgggc gccggtcgcg cggcgcaagt cggcggaggg atggttgagt
    11581 gcccacgggg ccagcaccag gacgatggcg ggaaccgggc gcaacgcgcg cgggatcgcg
    11641 ccggacagta gcgggagacc gagcccggca ccgagcgcgg cggcgacggc gcacgggagg
    11701 aagaggactt cgacgtcctc ggtgccgtac atcaagccga agccggccca ggcgccgccc
    11761 agcgtcaggc cggcgagcgc cggccggtcg cgcgacccac ggctggcgaa gcgtgccgcc
    11821 aggccggcga tcgcgagcgg cagaccgacg taaccggcgc cgctccacag catcgcgatc
    11881 aggcggcgcc agcctgcgag ctcgagccag ccggcggggc cgagcacgta cagccggtat
    11941 ccgcgcgtcg tcacgtggtt ccacagcgcg agcggatgat ccggcgccgc ccagttgacg
    12001 gcgggctcga gcgaggagcg ccacatcaac caggtgtagg cggcaaccgg gatcgccgcg
    12061 gccagcccgg tggccccggc cacgcacctg cggccgatcg gctgccgcgg cccgccgtcc
    12121 caggccagga cgacgagtgc cggcagggcg agcagcaccg tcgggcggtg ggccagtccg
    12181 acgccgacca gcgcggccgc gacccaggtc catcgccggc gagcgcgccg gtcgaccgcg
    12241 ccgtgccact cgtcggcgac gaccaacagc gccgtgacga ggaacaggtg gagcgtgtag
    12301 acttcggcca ccgtcgcgat cgcccaaacg tcccgcgtta cggcgagcgc cgcacccgcg
    12361 gcgagcgccg cgagcgcgga gccggtccag cgtgcgacga gcaccgcgac caggccggcg
    12421 gcgagcgcgc cgaagacgga cgacatcagg ttgacccgcc aggccgcctc tcccagcggc
    12481 acggcgaggg cgaccttcgc cagcgccgtg tagagcggat agccgggggg gtgggccaca
    12541 cccaggctgt cggcggcggc gatcagctcg ccgccgtcgc cgtaatagac cgtcgggcag
    12601 gcggtccaga catagagcag cacgaccgcc gaggcgcagc cgagccccgt cgcgtacggc
    12661 cgagacccgt cgcgccgcac catccccggc gctactcggc gagtgccgcg acggcctcgg
    12721 cgatccggcg gacgccctca cggatccggt cgacggagac cgcatacgag atgcggacgt
    12781 ggtccggggc gccgaacgcg gtgcccggaa ccaccgcgac ccgcgcctgg gccaacaggt
    12841 actcggcgaa acggtcgccg ccgtcgatcc gccgatcgcc ggcccggcgg ccgatcagcc
    12901 cgtcgacgct ggggaagaca tagaacgcgc cgtcggggac caggcaacgg acaccgtcga
    12961 cctcccccag caaacgcacg atctcgtcgc gccgccgttc gaactccgcc acgcgccgcg
    13021 agacctcgtc ctcgcacgag gcgagcgcct cgatgctggc ccactggctg atcgagcagg
    13081 cgttgctggt gctgtggctc tgcaagcgcg acatcgctgc aatcacctcc tccggacccg
    13141 cggcgtaacc gatgcgcaga ccggtcatcg agtagctctt gctcatcccg tcgatcacga
    13201 ccgtgcgctc gcggatctcg ggggagagct cggcgatgct cgtgaacggc aacccgtcgt
    13261 agaccagccg cgagtagatc tcgtcggcga gcacccagat cccgcgctcg acgcagaatc
    13321 gcgcgatcgc atcgagctcg tcgcgccggt agcaggcgcc ggtcgggttc gacgggtagt
    13381 tgaggaccag cgctcgagta cgcgggctcg cggcccgctc gagcgcgtcg accgtcagct
    13441 tgaagccgcc ctgctcagga caagggacga tcaccggcgt accgccggcc agggccactt
    13501 gctcgggata ggacacccag tacggggcgg gcacgagcac ctcgtcgccc gcgtcgacca
    13561 gggcgaggaa ggcgaaatac agactggctt tggctccggt cgacaccagg atctgacccg
    13621 gcggatagtc gagaccgcgc gtgcgcgcca gcgaaccggc gatcgccgac cgcagctcgg
    13681 ggaggccggc gttcggcgtg tagcgcgtgc ggtcctgcgc gagcgcctcc gacgcggccc
    13741 gcttcgccgc ggcgggcacg ggaaagtcgg gctgacccgc gctgaagtcc agcacgtcga
    13801 tgccttcggc gcgcatcgcc tgggccaggg cgctgatccg cagcgtcggc gacgatccga
    13861 tgcttgccgc acgtcgcgag accatcgtgt acttccgccc gcccgcttcg ctcagcgctt
    13921 cggcccgacc gcccgcttct tctgaaagat gaactcgcgc agctgattgc gctggaggtg
    13981 gatgctcgcc acgccgagcg gtgccagcgc ccgttcgagc gcccgcggat tgtgaccgta
    14041 gcgacgacga cgcgggccgc tgcccgcctg ccgctcgacg tgaccttcgt cgacgacgcg
    14101 gtagcgcggc gcgcggtcgg cgtgcggccc gtccttcggt ggatcgctgg ccgacatcaa
    14161 cagcgccgta ccaccgtcgg ccatcacacg gtgcaactcg gcgccgaact cgccgacccg
    14221 atcgggcggc aggaagtcac acatctccca gacgagtacc aggtcgaagc gcgaatcgtc
    14281 gaaggcgagg cggatcggcg gtaccgcggg cggctcgctc gttcgcgccg cgagatcgtc
    14341 ggggtcgcgc tccgccggtg gtggcggcgg cggcggcggc tcgagctccg cgacgctgat
    14401 gcgcgcaccg cgctcgccga aatagaccgc ggtgtcgccg cagaacggtc cgaggtcgag
    14461 cacttcgggc ttgtcgatcc gaccgatctt ctcgagcacc ttgccgagac ccgggcactt
    14521 ctgcaccgct tccccgcccg cggcggcgtc gggctggccg gacaacccgg tcgagctgcc
    14581 ggcacggctc cacgggaatt tcaccggtca gcccttggtc gaacctgcgc tccgcgtgat
    14641 cgagcccggc gggaggacac ccgatccgac cggcttggtg gcggtcgagg cgtgcgccga
    14701 aggcgacgcc gtcgcgggca cgccggtctt gcggtccatg tcgatgctcc ccttgaactt
    14761 cgcaccatcg gcgatcgcga cccgcggcgc ccgcacgtcg ccgtggaccg atccggaggg
    14821 cgctacctcg accttgtcgt cggcggtgat gttgccgacg acctgcccga cgacgacgac
    14881 gttcttggcg tggacctccg ccgtgatccg cccgttggcc ccgatcgtca gcgaatggtc
    14941 cttgacgagg atcttaccgt cgaccaggcc ctcgatcgtc aggtcctcgt ttccggtcag
    15001 cgtacccttg atctcgaccg attgtccgat gttcacgatc gactccattc ttccctccgg
    15061 ttcgggcgcg ggcgaggttg ctcgcgcggc cactggagag gacggctcgg cggagtccgt
    15121 ctccgccggc gatttcttgt tcaaccaggc catgcgtgag cctcctccgg acgctcgaac
    15181 ggcaggcccc gacccggatc cgccgtccgg cgcttcgaga cccgtcggat tatactcaat
    15241 cgggtcggct cgaccagcgc gggccacccg aatgtggcgt aaacgtcgcc ctttggcgga
    15301 gattctcgtt gcgacggatg cccccccagc ttcgtctctt gctcgtcacc cttccactcg
    15361 cggtcggtct ggccgacgcg tctccgaccg cggccgcgtc gaacgagctg aagcagtgga
    15421 tcgacggccc ggtgcgctac atctcccgca agcaggaggc caagctgttc cacgagctcg
    15481 agacggacga ggcgcgcgcc gcgttcatcg cccggttctg gcggcggcgc gatccggacc
    15541 ccgagacgct gaccaacgag taccgcgagt tgttctggcg gcgggtcaag gaggcgaatc
    15601 agagcttcct cgacagctcc aaacagggtt ggatgaccga ccgcgggaag atctggatcc
    15661 tctacgggcc accgacggac gtcgaggact acaccgacct gcaggcaaac gacgcggccg
    15721 ggcacggcgt gatccgctgg atctacgagg ggcgccccgg cgaacggatc gacatgcggc
    15781 cgatcgtcgt cgtcccgttc gagcgacaga ccgacggcga gtaccgcgtg tcgtacgatc
    15841 cgcagctggc gagcgtgttc ttcgacgcga cggggatcgc cgaaggccag aacgacatct
    15901 acgacaagtt cttcgagctc gccggcgcac cgcgccaatc cgagctgtcg gtgatgctcg
    15961 atctcggccg catgcaagag gtgccacctg ccgagcaagt gctgatcgaa tcggtcgaga
    16021 cgatggagtc ctacggcacg ctcgagctgc cggtagcggt caatcactac ttccgtcccg
    16081 aggacggtcg gccgctggcc gtgatcaacg tcgagctgac cgacctgggt cagatcgacg
    16141 atgccgcgat cctcgcccgt ttccggccgg tcgagcccac gcgcgagaca cgggtgctcg
    16201 gcgaggactc gttccgcctg gcgacggccg gcgactaccg gttggcgcag ggtcgtcttt
    16261 cgctcgagcc gggccgctat gacgtgaccg tcgtcgtcgc cgacccgatc cacgtacaga
    16321 ccgcgttgta caaggcggag ctcgacgtgc ccgaaacacc gcggacgctc agcctgagcc
    16381 agccggtctg ggccgcggag atggcgcagg tcgagtatcg ttcgctcgcg tcgtacgacg
    16441 agccgtttca cctcggcccg ttccgcgtgc tgccgttgct gacggcgacc taccaccgcg
    16501 gcgacagcgc gaagctgttc gtcgagacct acggggcaga gtacccgctg acggcgaccc
    16561 accaactcga gggcctcgag gcggacggga gctgggtcgc gctgggtcag ccggcggtga
    16621 tcgagcagtc ggccgccggc ctggcctggg aggtccagac cgggccgcgc tggccgctcg
    16681 gccgttaccg cgtccgggtg gaagtcgtcg acgcggccgg ccgcacggcc cggaccgaag
    16741 ccgagttcgc gctcgacgag gcggagctcg acggtccggc cgccgacacc gacgacggtg
    16801 cagcgggcga ttcggactcc tgacgcgtca tcgatcgcga ccaggcgagg cgatcgggta
    16861 tgctcggccc tccgccgatg tcgttaccgc gccgcgccgc cctgctgctc gccctgctcg
    16921 ccgcttgtcg gggcgtgccc ctcgccgcgt gcgaggcgga gaccgcgagc gatcccgacc
    16981 cgcgcggccc gaccgccggc gg
//
LOCUS       DASPGN010000275         7206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1452650, whole genome shotgun sequence.
ACCESSION   DASPGN010000275 DASPGN010000000
VERSION     DASPGN010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452650"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..786)
                     /locus_tag="VD788_08095"
     CDS             complement(13..786)
                     /locus_tag="VD788_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76265.1"
                     /translation="MNPRRGITAFLLLAVAFAPAVGAWAPETRVGMIDDAIKLMPRSL
                     RLALETHRQDVLRGALKPMIDEDGPEHRAPSADGSLDRQIEGEATALAALLGRQSEFD
                     AVSEQFGRLGHFVMDAAFPPGVSEDDGDSRYDHFAEFCEQRREKFRLVFYGHDDEQLA
                     VGDFHAFALEVIRRASSEDRELARAYSAAGDPPDPSAFDDRSIPFAVGALAYSRAVND
                     IVRAWLTVWRQVGGDLGRTPYLDNEPRRGDLSQSQPGGS"
     gene            complement(839..1630)
                     /locus_tag="VD788_08100"
     CDS             complement(839..1630)
                     /locus_tag="VD788_08100"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76266.1"
                     /translation="MRRLVGSIVTLVLLAVPVAVAQETADERQRAKEARIAEYLQKKE
                     QRRLDKDARRQEKVAATAPDGPTVAELGGDTLPRELARAQDAVRASAIGADPTIQRYL
                     DLIERQEASAHQLAAFGNFVADAGMAAEAEVYYNVALSLERGDPALWINYGTLMRQLG
                     DLPKAGSAYGRALEIDPNNALAHYNLATVLEAQGKYEDSISAYSVALMLDPSLGDPTV
                     NPAAANNKRLVAVKLMLYQQQTGSLGTPLVEIPEGGLPEEGDVDD"
     gene            complement(1658..2242)
                     /locus_tag="VD788_08105"
     CDS             complement(1658..2242)
                     /locus_tag="VD788_08105"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HXV76267.1"
                     /translation="MTDSLQPPKAGTVFEADMHRLDVGIRQLKVQYDMFFAGALPREP
                     LEQRQQVERIVKRYANNSTLKYAESFRLNSLISRFNSLAELWSKTVRRREEGAAPAAA
                     LANPHAPREQLVARCRLSETRDDDPELRRLYQRFVETRRRMNDGNREISYQKFVRGIA
                     GQTERLRNQHGCAEMELRVVVHDHKVLLKVRSGG"
     gene            2298..3761
                     /gene="selA"
                     /locus_tag="VD788_08110"
     CDS             2298..3761
                     /gene="selA"
                     /locus_tag="VD788_08110"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HXV76268.1"
                     /translation="MQRPKPARAGKRPDSARTPADIPAVSRLLELPALGRLSGRHGHR
                     LVRDVLRDHLARLRERLRDGVLDPAAARRALAARTIAAEVARAVETTVAPAPRVVINA
                     TGVVIHTNLGRAVLSAEAAARVAEVASRYVDLEYDLARGRRGHRADHLRPLMARLFAG
                     HDFVVVNNNAAAIMLALRALARGREVIVSRGELVEIGGSFRVPDILSASGARLREVGT
                     TNRTRLDDYRAAVSPKSGALLKVHTSNFRVVGFAEGTSIEALASLARDSALPLVVDWG
                     SGDLVDLAPLGVTDELPAADVLRAGADLVTFSGDKLLGGPQAGFVVGRAELIARLRRD
                     PLARVCRVDRLTIAATHATLSAYVRGRAFDEVPTLRMLAAPAEAIGRRARRVRREVVA
                     RGGQGKLLRVVAGVSRTGGGSSPAGERPTFLLAVEWPGGDAAPLERRLRGGDPPLIGR
                     LQEGRLLLDLRTVVPEQDARLSERLSEELAALGPVRR"
     gene            complement(3751..4827)
                     /gene="ychF"
                     /locus_tag="VD788_08115"
     CDS             complement(3751..4827)
                     /gene="ychF"
                     /locus_tag="VD788_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXV76269.1"
                     /translation="MKIGLFGFPMTGKSTLFHLLTGAGPAAHSTRGEARIAVSKVPDR
                     RLDGLAAIYQPKKYTPATIEYLDLPAMEKGQAADVLPLDQLRTADALAHVVRAFEDDA
                     IPHAEGSIDPARDVATMETEFLLADHSIAERRVEKLVLQVQKTQRDEDKKELELFRKV
                     LDHLEREIPLRDVAFDDAERLRLRGYTFLSLKPLLIVVNAGELAARRLVEGAAAFGLA
                     SFAARPSTAVVALSAKIESEIADLDESDRFAFMQDLGISQSALDRMIRASYELLGVIS
                     FFTAGESEVRAWTIRQATPAREAAGTIHSDMERGFIRAEVIAYDDLIAAGSWAGGREK
                     GTLRLEGRDYPVKDGDVIVFRFNV"
     gene            4952..5428
                     /locus_tag="VD788_08120"
     CDS             4952..5428
                     /locus_tag="VD788_08120"
                     /inference="COORDINATES: protein motif:HMM:NF037472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rap1a/Tai family immunity protein"
                     /protein_id="HXV76270.1"
                     /translation="MKNDLLTCILLCVVSTTPAWPAPANERVDGHALLAVCSNAVRLF
                     DGEPVEAGRESDPPYCLGFVTAVRQAAEQLAERGAAYEQGGVRCSWHYETGWISQIES
                     VAAELFRPVCYPDGLESERLVRLVVQFMRSRPDELHLSGAALVSAAVSQTFPCPSE"
     gene            complement(5619..>7206)
                     /locus_tag="VD788_08125"
     CDS             complement(5619..>7206)
                     /locus_tag="VD788_08125"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV76271.1"
                     /translation="YEPREGGGSAAVSVHDGKLLLDYAGQRTPLAAAADRVFRNAEAG
                     VTVRYFGRAGTVEGLSIERAAGEPVRMGLADEGELARLDPADYVERSARPPTVNWPAF
                     RGVNRDGIGDGAEPPADFDPRTGAGVAWRVELPGLGNSSPVVWGDRVFVTTAVARGGS
                     VPLRTGLTGDGDEIDEDSEHRWLVLAYDKTTGKKLWETEVGRGVPLTRRHFKATQANS
                     SPATDGEHVVAVFPTAGLACLGIDGRIHWKQELGGLNAGGFNDPGIEWGYASSPIIHD
                     GKVILQVDIHDGPYVAAWELDSGKLAWRTARPGIAPSWSTPAIWPTPDGDELVLNASV
                     IHGYDPDDGKLLWALGPTSVQVVAAPVVGDVRLFVSSGYPPVRPVYAVKPGIEGVHEI
                     DFDSNEADPALAWQLERGGAYMPTPLLYRGLLYVVHHNGRIVAHDARSGAPVYRARLS
                     SGGTCTSSPVAADGKIYQGTEEGTLYVLAAGPEHRELAVREFDAPLMATPAISDGLLI
                     VRTPAQLIALGGRRGPSDAP"
BASE COUNT          982 a         2541 c         2573 g         1110 t
ORIGIN      
        1 cgggctgttc catcaggatc ctccgggctg cgattgcgag aggtcgcccc gccgcggctc
       61 gttgtcgagg tagggcgtgc gccccaggtc gccgccgacc tgcctccaga cggtcaacca
      121 ggcgcggacg atgtcgttga ccgcacgcga gtaggccagc gcgccgaccg cgaacggaat
      181 cgagcggtcg tcgaacgccg acggatcggg cgggtcgccg gcggccgagt aggcccgagc
      241 cagctcgcgg tcctcggagc tggcgcgtcg gatcacctcg agcgcgaacg catggaagtc
      301 gccgacggcc agctgctcgt cgtcgtgtcc gtagaacacc aggcggaact tctcgcggcg
      361 ttgctcgcag aactcggcga agtgatcgta acggctgtcg ccgtcgtcct cgctcacgcc
      421 cggcgggaag gcggcgtcca tcacgaagtg gccgagccgg ccgaactgct cggagaccgc
      481 gtcgaactcg ctttgccggc cgagcagcgc ggcaagtgcc gtcgcctcgc cttcgatctg
      541 ccggtcgagc gagccgtcgg ccgacggggc ccggtgctcc ggcccgtctt cgtcgatcat
      601 cggcttgagc gccccgcgca ggacgtcctg tcgatgggtc tcgagcgcca gccgcagcga
      661 gcgcggcatc agcttgatcg cgtcgtcgat catgccgacg cgcgtttcgg gagcccacgc
      721 accgaccgcg ggcgcgaatg cgaccgcgag gagcaggaac gccgtgatac cccgccgagg
      781 gttcatagga cgcggattct agacaagccc gcgcgtgcgg gcaaacggcc gtttgcgatc
      841 agtcgtcgac atcgccctct tcgggcaagc cgccttccgg gatctccacc agcggcgtgc
      901 cgaggctgcc ggtctgctgc tggtacagca tcagcttgac cgcgaccagt cgcttgttgt
      961 tggccgcggc cgggttgacc gtcgggtccc cgagcgacgg gtcgagcatc aacgcgacgg
     1021 agtacgcgct gatcgagtct tcgtacttgc cctgggcttc gagcacggtc gccaggttgt
     1081 agtgtgcgag cgcgttgttc gggtcgatct ccagcgcccg gccataggcg ctgccggcct
     1141 tgggcaggtc gccgagttga cgcatcagcg tgccgtagtt gatccacagc gccgggtcgc
     1201 cgcgctcgag gctcagcgcg acgttgtagt agacctcggc ctcggcggcc atgccggcgt
     1261 cggcgacgaa gttgccgaac gccgcgagtt ggtgcgccga ggcctcctgc cgttcgatca
     1321 ggtcgaggta gcgctggatc gtcggatcgg cgccgatggc gctggcccgc accgcgtcct
     1381 gcgcccgcgc cagctcgcgc ggcagcgtgt cgccccccag ctcggccacg gtcggtccgt
     1441 cgggggcggt cgccgcgacc ttctcctgcc ggcgggcgtc cttgtcgagg cgtcgctgct
     1501 ccttcttttg caggtactcg gcgatgcggg cttctttggc ccgctgccgc tcgtcggccg
     1561 tctcctgagc gacggcgact ggcacggcga gcaagacgag cgtcacgatc gatccaacga
     1621 ggcgtctcat ccgaaccccc ggcgtcgccc gtcggagcta accacccgag cgaactttga
     1681 gcagtacttt gtggtcgtgg acgaccacgc gcagctccat ttcagcgcac ccgtgctgat
     1741 tgcgcaagcg ctcggtctgt ccggcgatgc cgcgaacgaa cttctggtag gaaatctcgc
     1801 ggtttccgtc gttcatccgc cggcgcgtct cgacgaaccg ctgatacagc cggcggagct
     1861 cggggtcgtc gtcgcgggtc tcgctcaacc ggcagcgcgc gacgagctgt tcacgcgggg
     1921 cgtgcgggtt cgccagcgcc gccgccggag cggcgccttc ctcgcgccgc cgaacggtct
     1981 tcgaccagag ttcggctaga ctgttgaagc ggctgatcag ggagttgagg cggaaactct
     2041 ccgcatactt cagcgtcgag ttgttggcgt agcgtttgac gattcgctcg acttgttggc
     2101 gctgctcgag cggctcgcgc ggcagggccc cggcgaagaa catgtcgtat tggaccttga
     2161 gctgacggat gccgacgtcc agccgatgca tgtcggcttc gaagacggta ccggctttcg
     2221 gcggctgcag cgaatcggtc atatgaggat cgcttccaac ggacgacgcg gtcgaatcta
     2281 aggatcggag accgggaatg caacgaccga agccagcgcg cgcggggaaa cggccggatt
     2341 cggcccggac accggccgac atccccgccg tctcgcgcct gctcgagttg cccgcgctcg
     2401 gccggctgag cggccgccac ggccaccgcc tggtgcgcga cgtcctccgc gaccacctgg
     2461 ctcggttgcg cgagcggctg cgcgacggcg tgctcgaccc ggcggcggcg cgacgcgcgt
     2521 tggccgcccg gacgatcgcg gccgaggtcg cgcgcgccgt cgagacgacc gtcgcgccgg
     2581 cgccgcgcgt ggtgatcaac gcgaccgggg tcgtgatcca caccaacctc ggccgcgcag
     2641 tgctgtcggc cgaggcagcc gcgcgcgtcg ccgaggtcgc gagccgctac gtcgacctcg
     2701 agtacgacct ggcgcggggg cggcgcggcc atcgcgcgga ccatctccgg ccgctgatgg
     2761 cgcgtctgtt cgccgggcac gatttcgtcg tcgtcaacaa caacgccgcg gcgatcatgc
     2821 tggcgctgcg cgcgctggcc cgcggccgcg aggtgatcgt ctcccgcggc gagctggtcg
     2881 agatcggcgg ctcgttccgc gtccccgaca tcctgtcggc gtcgggcgcc cggctccgcg
     2941 aggtcggcac gacgaaccgc acgcggctcg acgactaccg cgccgcggtc tcgcccaaga
     3001 gcggcgcgct gctcaaggtg cacaccagca acttccgcgt cgtcggcttc gccgagggga
     3061 cgtcgatcga ggcgctggcc agcctggcgc gcgacagcgc cctgccgctc gtcgtcgatt
     3121 ggggcagcgg cgacctggtc gacctcgcgc cgctcggtgt gaccgacgag ttgccggccg
     3181 ccgacgtgct gcgcgccggc gccgacctcg tcaccttcag cggcgacaag ctgctcggcg
     3241 gaccgcaagc cgggttcgtc gtcggccgcg ccgagctgat cgcccggctc cgccgcgacc
     3301 cgctggcccg cgtctgtcgc gtcgaccggc tgacgatcgc ggcgacgcac gccacgctgt
     3361 cggcctacgt tcgcgggcgc gcgttcgacg aggtgccgac gctgcgcatg ctcgcggcgc
     3421 cggccgaggc gatcggtcgc cgggcgcgac gcgtcaggcg cgaggtggtg gcgcgcggcg
     3481 gtcagggcaa gctgctgcgc gtcgtcgccg gcgtgtcgcg caccggcggc ggctcgtccc
     3541 cggccggcga gcgtccgacc ttcctgctcg ccgtcgaatg gcccggaggt gacgccgcgc
     3601 cgctcgagcg ccggttgcgc ggcggcgacc cgccgctgat cggtcggctg caggagggca
     3661 ggctgctgct cgacctgcgc acggtcgtcc ccgagcagga cgcgcggctg agcgaacggc
     3721 tgtcggagga gctggcggcg ctcgggccgg tcagacgttg aagcggaaga cgatcacgtc
     3781 gccgtccttg accgggtagt cgcgaccctc gagccgcagc gttcccttct ctcggccgcc
     3841 ggcccagctg cccgcggcga tcaggtcgtc gtaggcgatg acctcggcgc ggatgaagcc
     3901 gcgctccatg tcgctgtgga tcgtgccggc cgcctcgcgc gcgggcgtcg cctggcggat
     3961 cgtccaggcg cgcacctcgc tctcgcccgc ggtgaagaac gagatcacgc cgagcaactc
     4021 gtaggaggcg cggatcatcc ggtcgagcgc cgactgcgag ataccgaggt cctgcatgaa
     4081 ggcgaaccga tcgctctcgt cgaggtcggc gatctccgat tcgatcttgg ccgacagggc
     4141 gacgacggcg gtcgacggtc tggcggcgaa cgaggcgagg ccgaacgccg ccgcgccctc
     4201 gaccagtcgg cgcgcggcga gctcgccggc gttgaccacg atcaacagcg gtttcaacga
     4261 cagaaaggtg tagccgcgca gccgcaaccg ctcggcgtcg tcgaacgcga cgtcgcgcaa
     4321 cgggatctcg cgctccaggt gatcgagcac cttgcgaaac agctcgagct ccttcttgtc
     4381 ctcgtcgcgt tgggtctttt gcacctggag caccagcttc tcgacgcgac gctccgcgat
     4441 cgagtgatcg gccagcagga attcggtctc catcgtggcg acgtcccgcg ccgggtcgat
     4501 cgacccttcg gcgtgaggga tcgcgtcgtc ttcgaaggca cggacgacgt gcgccagcgc
     4561 gtcggccgta cggagctgat cgagcggcag cacgtccgcc gcctggccct tctccatcgc
     4621 cggcagatcg aggtactcga tcgtcgccgg cgtgtatttc ttcggctggt agatggcggc
     4681 cagcccgtcg agccgacgat ccggcacctt cgacaccgcg atccgcgcct ctccgcgtgt
     4741 cgagtgggcg gccggcccgg cgccggtcag caggtggaac agcgtcgact ttccggtcat
     4801 cggaaaaccg aacagcccga tcttcatgcg cggtcactcc cgtttcgcgc ggggagcata
     4861 gcgaacggtc cgcgcccgcg ccacgctcgt cacgcgcgcg gagcggccgt atagtgccag
     4921 tacggcccga cgcgccgcaa cgggtgagcc gatgaagaac gatctcctga cctgcatcct
     4981 gctctgcgtc gtctcgacga cgccggcgtg gccggcgccg gcgaacgagc gggtcgacgg
     5041 ccacgcgttg ctcgcggttt gctcgaacgc ggtgcgcctg ttcgacggcg agccggtcga
     5101 ggcgggacgg gaaagcgacc cgccgtactg tctcggtttc gtcaccgccg tgcgtcaggc
     5161 ggccgagcaa ctcgccgagc gcggcgccgc gtacgagcag gggggcgtcc ggtgctcgtg
     5221 gcactacgaa accggctgga tcagccagat cgagtcggtg gccgccgagc tgtttcggcc
     5281 ggtctgctac ccggacggcc tcgaaagcga gcggctcgtg cgtctcgtcg tgcagttcat
     5341 gagatcccgt ccggacgagc tgcacctctc gggcgccgca ctcgtgtcgg ccgccgtctc
     5401 ccagaccttt ccctgcccga gcgagtgaag cggttcagtc ctccgcccgc tcccagaccg
     5461 gtccgcacca cggcggcgag ccggtcgcgt cggcccggta gtcggccggc agcggcgtcg
     5521 gctggcgcgc cgcgtacctg agaagcaacc tcggaagcgg cgtcacgcga ggttcgcacg
     5581 acgcgaaaga ggacggtttc tgtccagggc gtcccgggct acggcgcgtc ggatggcccc
     5641 cggcgtccgc cgagcgcgat cagctgtgcg ggagtgcgca cgatcagcag cccgtcggag
     5701 atcgccggcg tcgccatcag cggcgcgtcg aactcacgta ccgccagttc gcggtgctcg
     5761 ggcccggcgg cgaggacgta cagcgtccct tcttcggtgc cctggtagat cttgccgtcg
     5821 gccgcgaccg gcgacgaggt gcaggtgccg cccgagctga gccgcgctcg gtagaccggc
     5881 gccccggaac gcgcgtcgtg ggcgacgatc cgcccgttgt ggtgcacgac gtagagcagg
     5941 ccgcggtaga gcaacggcgt tggcatgtag gcgccgccgc gctcgagctg ccaggccagc
     6001 gccgggtcgg cttcgttcga gtcgaagtcg atctcgtgca cgccctcgat gccgggcttg
     6061 acggcgtaga ccggacgtac cggcgggtag cccgaggaga cgaacaggcg cacgtcgccg
     6121 acgaccggcg ccgcgacgac ctgtaccgac gtcgggccga gcgcccacag cagcttgccg
     6181 tcgtcggggt cgtagccgtg gatcaccgac gcgttgagca ccagctcgtc gccgtccgga
     6241 gtgggccaga tcgcgggcgt cgaccacgac ggcgcgatgc cgggcctcgc cgtccgccag
     6301 gcgagcttgc cgctgtcgag ctcccacgcg gcgacgtacg ggccgtcgtg gatgtcgacc
     6361 tgcaggatca ccttgccgtc gtggatgatc ggcgaggacg cgtagcccca ctcgattccc
     6421 gggtcgttga agccgccggc gttcaagccg cccagctcct gcttccagtg gatccggccg
     6481 tcgataccga gacaggcgag acccgcggtc gggaagaccg cgacgacgtg ctcgccgtcg
     6541 gtcgccgggc tcgagttggc ctgggtcgcc ttgaagtggc ggcgggtcaa cgggacgccg
     6601 cggccgacct ccgtctccca cagcttcttg ccggtcgttt tgtcgtaggc caacaccaac
     6661 caacggtgct cggagtcttc gtcgatctcg tcaccgtcac cggtgagccc ggtgcgcagc
     6721 gggacgctgc cgcctcgggc gacggccgtg gtgacgaaga cgcggtcacc ccagacgacg
     6781 gggctcgagt tgcccagccc cggcagctcg acgcgccacg cgacgccggc acccgtccgc
     6841 gggtcgaagt cggcgggcgg ctcggcgccg tcgccgatcc cgtcacggtt gacgccgcgg
     6901 aacgcgggcc agttcaccgt cggtggccgc gcggagcgct cgacgtaatc cgccgggtcg
     6961 aggcgggcca gctcgccttc gtcggcgaga cccatccgca cgggctcacc ggccgctcgt
     7021 tcgatcgaca gcccctcgac cgtaccggcg cggccgaagt agcggacggt cacgccggct
     7081 tcggcgttgc ggaacacgcg gtccgccgcc gccgcgagcg gggtccgttg gccggcgtag
     7141 tcgagcagca gtttcccgtc gtggacgctg accgcggccg agccgccgcc ctcgcgtggt
     7201 tcgtag
//
LOCUS       DASPGN010000276         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_868980, whole genome shotgun sequence.
ACCESSION   DASPGN010000276 DASPGN010000000
VERSION     DASPGN010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868980"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VD788_08130"
     CDS             <1..266
                     /locus_tag="VD788_08130"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HXV76272.1"
                     /translation="QESDGSWVVDAAAHVEELEELFDVEFGERDYDTVGGLVVSGFGR
                     VPNVGESMISHGLNVEVLMADHRRVRKVRVRRDSSEQKATGGA"
     gene            263..1180
                     /gene="era"
                     /locus_tag="VD788_08135"
     CDS             263..1180
                     /gene="era"
                     /locus_tag="VD788_08135"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HXV76273.1"
                     /translation="MSGPQSGNPAASRSGFAALVGWTNVGKSTLLNRLVGVRLAAVAD
                     VAQTTRQRIQGATTIAGRGQIVFVDTPGIHQPRHRLNRAMVETVHRAVEEVDLVLLVL
                     DAQCGIGPGDRRAAELVRRRARARIAVLNKVDRLRQKSRLLPMIEQLVDEWGFAEVVP
                     VSALTGDGCERLLEVVLAQLPVGPPLFPDDYLTDQAERVIAAEWIREKLLDEIRQELP
                     HATAVVIDRWTERSDGLLELEASVLVERESQKKIVIGREGQLLKRVGSAARIELERFF
                     ARRVHLRLWVSVCDGWRDDDAVLHRLGLD"
     gene            1259..2026
                     /gene="recO"
                     /locus_tag="VD788_08140"
     CDS             1259..2026
                     /gene="recO"
                     /locus_tag="VD788_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXV76274.1"
                     /translation="MRLLNDEAWVLETRELGEADLIVALFCREHGSVRGVARAARKSR
                     RRFGGSLAPLTRVRLTWGEKAGRELHRIDGIECTRSFASMQREPVVQAAVAVVCEVAQ
                     ALAREGQPGPREFDLIAAVAQALEAGCDPWIAVRYLEYWMLRLHGVLPDFDRCASCGR
                     ELGRRPVARVDLHGIVRCRECAGGRVGERVHLLRDVERALLGEFRSCAPLAVTGDARL
                     AGPSGTLDVLLRGSLEAFVERRFRSYRHLRALASDRR"
BASE COUNT          304 a          662 c          783 g          350 t
ORIGIN      
        1 agcaggaatc cgacggttcg tgggtcgtcg acgccgccgc gcatgtcgag gagctggagg
       61 agctgttcga cgtcgagttc ggtgagcgag attacgacac ggtcggcggg ctggtggtct
      121 ccggcttcgg gcgcgtaccg aacgtcggcg aatcgatgat ctcgcacgga ttgaacgtcg
      181 aggtgttgat ggccgaccac cgtcgcgtgc gcaaggtccg cgtgcgtcgg gactcctccg
      241 agcagaaagc gaccggcgga gcgtgagcgg gccgcaatcc ggcaacccgg cggcgagccg
      301 ttccggcttt gccgcactcg tcggttggac gaacgtcggc aaatcgaccc tgctgaacag
      361 gctcgtcggc gtcaggctcg ctgcggtagc cgacgttgcg caaaccacgc gccagcgaat
      421 ccagggtgcg acgacgatcg ccggccgcgg acagatcgtc ttcgtcgata cgccgggcat
      481 ccaccaaccc cgtcatcggc tgaatcgcgc gatggtcgag acggtacacc gcgcggtcga
      541 ggaggtcgat ctggtgctgc tcgtgctcga tgcgcagtgc ggcatcggtc cgggcgaccg
      601 acgggcggcg gagctggtgc gtcgccgcgc gcgggcgcgg atcgcggttc tcaacaaggt
      661 cgatcggctg cgccagaaga gtcgcctgct gccgatgatc gagcaactgg tcgacgagtg
      721 gggcttcgcc gaggtcgtgc cggtatcggc cctgaccggt gatggatgcg agcgcttgct
      781 cgaagtggtg ctcgcgcagc tgcccgtcgg gcctccgctg ttccccgacg actacctgac
      841 cgaccaggcg gagcgcgtga tcgcggcgga gtggatccgc gagaagctgc tcgacgagat
      901 ccgccaagag ctgccccacg ccaccgccgt ggtgatcgac cgctggaccg agcggtccga
      961 cggactgctc gagctcgaag cgtccgtgct cgtcgagcgc gagtcccaga agaagatcgt
     1021 cattggccgg gaggggcaac tgctcaagcg cgtcggcagc gcggcgcgca tcgagctcga
     1081 gcggttcttc gcccgtcgcg tccatctgcg gctgtgggtc agcgtgtgcg atggctggcg
     1141 cgacgacgac gccgtgctgc atcgactggg gctcgactga cctgctatca ttcggccttt
     1201 ccgagggctc gtgcgccgtg caagcggcgg cgtcatgtgc cctccgcggc gaatccgctt
     1261 gagattgctg aacgacgagg cgtgggtgct cgagacccgc gagttgggtg aggcggactt
     1321 gatcgtcgcc ctgttctgcc gagaacacgg tagcgtccgc ggcgtggctc gtgccgcgcg
     1381 caaaagccgg cgccgcttcg gcggctcgct cgctccgctg actcgcgttc ggctgacctg
     1441 gggcgagaaa gcgggccgcg agctccatcg aatcgacggg atcgaatgca cccggtcgtt
     1501 cgcctcgatg cagcgcgagc cggtcgtcca agccgcggtc gcggtggtgt gcgaggtggc
     1561 acaggcactc gcccgggagg gccagcccgg accgcgcgag ttcgacctga tcgccgcggt
     1621 ggcgcaggcg ctcgaggctg gctgcgaccc gtggatcgcc gtgcgctacc tcgagtactg
     1681 gatgttgcgg ctgcacggtg tgctgcccga tttcgatcgt tgcgcgtcgt gcggccgcga
     1741 gctgggaagg cgaccggtgg cccgggtcga cctgcacgga atcgtccgct gccgggaatg
     1801 cgcggggggg cgcgtcgggg aacgggtgca cctgctgcgg gatgtcgagc gtgcgctgtt
     1861 gggcgagttc cgcagttgcg caccactggc ggtaaccggt gacgcgcggt tggccggtcc
     1921 gtccggaacg ctcgacgtcc tgctccgcgg ttcgctcgag gcgttcgtcg agcggcgctt
     1981 ccgcagctac cggcatctgc gggcgctggc gagtgaccgg cggtgagcgg caagaacttc
     2041 caacagctgt tgctggcgat ccagcactac tgggcgacgt gcggatgcgt gatccagca
//
LOCUS       DASPGN010000277         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_869276, whole genome shotgun sequence.
ACCESSION   DASPGN010000277 DASPGN010000000
VERSION     DASPGN010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869276"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VD788_08145"
     CDS             complement(<1..678)
                     /locus_tag="VD788_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76275.1"
                     /translation="MPRVTYSENRSRASPLWMLAGFVCATGVTLVASLAGSWDTALSP
                     AALALVALAGVAGAFPVRFAERKTAFTPTHPFILCALGAGGVVPAAAAAVAGVIGASL
                     RSNCRITPVRVVFNTGAGLVAAGAAAGVFVGLGGVPGRTWSVLLGPLAGAASAYFLCN
                     TTLVALAVALDTRAGLLGTWVTTFAWTIASSFAGFSVAVGLLVAVERFGVPGILLAVP
                     PVAVLVQF"
     gene            1012..1449
                     /locus_tag="VD788_08150"
     CDS             1012..1449
                     /locus_tag="VD788_08150"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HXV76276.1"
                     /translation="MGRLANILVVCTRNSARSQIAEAYLRRHVGDRFDVYSAGVDPST
                     VHPLTRRVLEEAGFDVSRHTAKNVRDFLGRLPVHTLIVVCDKADQRCPSTFPGLVERL
                     FWPFDDPAAATGSDEERLAAFRRVRDRIEARVRDWVGAGQTRA"
     gene            1471..3072
                     /locus_tag="VD788_08155"
     CDS             1471..3072
                     /locus_tag="VD788_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HXV76277.1"
                     /translation="MNAIADGRLRREIERRRTFAIISHPDAGKTTLTEKLLLYGGAIR
                     QAGAVRARRAARHATSDWLELEKQRGISVSSSVMTFEHQGLRVNILDTPGHHDFGEDT
                     YRTLTAADSAVMLIDAAKGVEPQTRKLFKVCRLRGIPIFTFINKMDRQGRDPLDLMGE
                     IEDVLGIHATPINWPVGAGSAFRGVYDRLTKRLLLFHGGAHGTAEVDEEIIEGAPNNP
                     AVQRALGTWADEVLESLELLDAAGEQCDADRVLRGELTPMFFGSALTNFGVLPFLERF
                     LRMAPAPYARRATVGPIDPGTDEFSGFVFKIQANMDPDHRDRVAFVRVCSGRFVKGAT
                     TMHVRTRRKLRLANSTLLMAQERHEVEEAYPGDVVGLFDPGVFRIGDTLSDRGDFEFE
                     GIPRFSPEHFAKVRVVEVLRRKALSKGLEQLTQEGLVQLLHEPDGGSSAIVLGAVGPL
                     QFEVLKFRLQSEYGVELKLSPMNYSIARWVRGQFDPVVFTHSIGVKVLEDRERRPVLL
                     FDNANTLRWNLEKHPGLDLVETGDF"
BASE COUNT          578 a         1142 c         1192 g          504 t
ORIGIN      
        1 gaactggacg agcaccgcga ccggcggtac ggcgagcagg atgcccggaa caccgaagcg
       61 ctcgaccgcg acgagcagac cgacggccac ggaaaacccg gcgaaggacg aggcgatcgt
      121 ccaggcgaag gtcgtgaccc aggtaccgag aagaccggcc cgcgtgtcga gcgccaccgc
      181 cagtgcgacc agcgtcgtgt tgcagaggaa gtaggcggag gctgcgccgg cgagcgggcc
      241 gagcagcacg ctccaggttc ggccgggcac gccgcccagc ccgacgaaga cgccggcggc
      301 ggcacccgcg gcgacgagcc cggcaccggt gttgaacacc acgcgtacgg gcgtgatgcg
      361 gcagttcgac cgcagactcg cgccgatgac cccggccacc gcggcagcgg cggccggcac
      421 gaccccgccg gcgccgagcg cgcacagaat gaacggatgc gtcggcgtga aggcggtctt
      481 gcgctcggcg aagcggacgg gaaacgcccc ggcgacaccg gccagcgcga cgagcgcgag
      541 cgccgccggg gaaagtgccg tgtcccagga gcccgccagc gacgcgacca acgtcacacc
      601 ggtggcgcag acgaaaccgg cgagcatcca caacggtgat gcgcggctgc ggttttcgct
      661 gtacgtgacg cgcggcatgt cgaacacccc ccgggcacac gacccgtcgg gaagcgatcc
      721 aagctgccag cgcaagttgc aagaggcata ccgccgcggg tacacgacga atcggcgtgc
      781 gccggcgcag cgacgtgtcg ccggtcgcga cagacccggt cgccacagga cccggggcgg
      841 cgagagcccc cgtcgtatca atcggttgcg cggtgtccgg tttcccggcg cgagatgtcg
      901 cggccggaga cagcggcgta cgcaaatgta acattgtcgt gacgtccgcg cgccgtcctg
      961 gcgcgagcgt tcgttccttc gctaagctgg agcgtctcga cggagtggca ggtgggtcgc
     1021 ctcgcgaaca tcctcgtcgt ctgtacgcgg aactcggcgc ggagccagat cgccgaagcc
     1081 tacttgcgca ggcacgtcgg cgatcgtttc gacgtgtaca gcgcaggggt cgatccgtcg
     1141 accgttcatc cgctcacccg gagagtgctc gaggaggccg gcttcgacgt ctcgcgtcac
     1201 acggcgaaga acgtgcggga ttttctcggg cgcctgccgg tgcacacgtt gatcgtcgtc
     1261 tgcgataagg ccgaccagcg gtgccccagc accttccccg gcttggtcga gcggttgttc
     1321 tggccgttcg acgacccggc cgcggcgacc ggaagcgacg aggagcggct ggcggcattc
     1381 cgccgcgttc gcgatcgaat cgaggccagg gtccgcgatt gggtcggggc gggacaaacc
     1441 cgggcgtgac gcctatagta tcggcccgac atgaacgcga tcgccgacgg ccgactgcgg
     1501 cgggagatcg agcgacggcg cacgttcgcc atcatctcgc accccgacgc cggtaagacg
     1561 accctgaccg agaagctgct gctgtacggc ggagcgattc gccaggccgg cgcggtccgg
     1621 gcgcgccggg ccgcgcggca cgccacgagc gactggctcg aactcgagaa gcagcgcggg
     1681 atctcggtca gtagctcggt gatgaccttc gagcaccagg ggctgcgcgt caacatcctc
     1741 gacacgccgg ggcaccacga tttcggcgag gacacctacc gcacgcttac cgccgccgac
     1801 agcgcggtga tgctgatcga cgcggccaag ggggtcgagc cgcaaacacg caagctgttc
     1861 aaggtctgcc ggctgcgggg cattccgatc ttcaccttca tcaacaagat ggaccggcag
     1921 ggtcgcgatc cgctggactt gatgggcgag atcgaggacg tgctggggat ccacgcgacg
     1981 ccgatcaact ggccggtcgg cgccgggtcg gcgttccgcg gggtctacga ccggttgacc
     2041 aagcggctgc tgttgttcca cggtggcgcg cacggcaccg cggaggtcga cgaggagatc
     2101 atcgagggtg cgccgaacaa cccggccgtc cagcgcgcgc tcggcacgtg ggcggacgag
     2161 gtgctcgaat cgctcgagct gctcgacgcg gcgggcgagc aatgcgacgc cgaccgggtg
     2221 ctgcgcggcg agttgacgcc gatgttcttc ggcagcgcgc tgacgaactt cggcgtgctg
     2281 cctttcctcg aacgatttct gcggatggcg cccgcgccct acgctcgccg agcgaccgtg
     2341 ggtccgatcg acccggggac cgacgagttc agcggcttcg tcttcaagat ccaagcgaac
     2401 atggatcccg accaccgcga tcgggtcgcg ttcgttcgcg tctgctcggg tcgtttcgtc
     2461 aagggcgcga cgacgatgca cgtgcgcacc cgaaggaagc tacggctggc gaactccacg
     2521 ctgttgatgg cccaggagcg ccacgaggtc gaagaggcct acccgggcga cgtcgtcggt
     2581 ctgttcgatc cgggcgtctt ccgcatcggc gacacactga gcgaccgggg tgacttcgag
     2641 ttcgagggca tcccccgctt ctcgccggag cacttcgcca aggtgcgggt ggtcgaggtg
     2701 ctgcggcgca aggcgttgtc caaggggctc gagcaactga cccaggaagg cctggtccag
     2761 ttgctccacg agcccgatgg cggcagttcg gcgatcgtgc tgggcgcggt cggaccgctc
     2821 caattcgagg tgctcaagtt ccgcctgcag agtgaatacg gcgtcgagct gaaactcagc
     2881 ccgatgaact actcgatcgc ccgctgggtc cgcggccagt tcgacccggt ggtcttcacc
     2941 cactcgatcg gcgtcaaggt gctcgaagat cgcgagcgac gcccggtgct gttgttcgac
     3001 aacgccaaca cgctgcgctg gaacctggaa aagcacccgg gtctcgatct ggtggaaacc
     3061 ggcgacttct gaccgtcagc ccgcacgacg catcgacccc gaagtgcacg acgatccgtt
     3121 tcacacagcg cctcgtagag ctccgatcgc cgccgcgcca ttctccaggt gctgctcctc
     3181 ccgcccgcgc cgatggactg tactcgacgg ccgtcgcagc gccgcgggcc gcgggtggtt
     3241 gaccgcggat tcccactggc gtatatggtg gtggcccttt tcgcctcgaa ctcgacggtg
     3301 gaccatgcgt gtacgacttc tcggcctgct gctgctgctc ccgttcgtcg gcgtcgcgat
     3361 tgcgcaggac gccaacgagt gcgacgaacc gggctacccc gacgtgatcg tcggtg
//
LOCUS       DASPGN010000278         7519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_986210, whole genome shotgun sequence.
ACCESSION   DASPGN010000278 DASPGN010000000
VERSION     DASPGN010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986210"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /locus_tag="VD788_08160"
     CDS             <1..738
                     /locus_tag="VD788_08160"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV76278.1"
                     /translation="VFRHNDVEDLAQKLDRVPDGIDRVVLIFDGIFSMRGDHAPLDHI
                     ASLSEHHAPRFRDGVVTVMDDSHGLGAYGDSGRGTEEHCGALADVLVGTFGKAFGVNG
                     GFVAGSGELVEAIRQRADTYIYTNPLGAADCAAAVEAVKLVDGDVGRERLATLRRRTA
                     RFRAGLAQLGLESIDGPHPVVPLLVRDTGRTRRTVEGLFERGVLAVGLTFPVVPRGDE
                     TIRFQVNAAHTEHDIDQVLAAVEQASR"
     gene            complement(735..3038)
                     /locus_tag="VD788_08165"
     CDS             complement(735..3038)
                     /locus_tag="VD788_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HXV76279.1"
                     /translation="MRLSIAKLRDARHVSELLGMLVILFGVVSLLALLSYSQADSTWF
                     KLQPDDLPTANWIGRVGATLAETYLQLFGVGAFSLPFLLAFAGWYRLKPPAQPIGWGR
                     IVGFSLLALAVVALAELLFRPMLYGGEVFDHPGGYVGWRFASLLRALLNTFGAVLFCV
                     TTIVGVTVLFGRVSLVRSAEIVAALCVRGVAWLGDYWREWREELAADEAGAARASDDD
                     ARQTAPRRPSITLGAAGAAAAPSTPAPPAPALVPRGAVEPAEVAAPAPVEPKQASLPL
                     DRLKRGYSLPPLELLKEPENQRREDEQELLERAEQITHKFREFSIEGTVVAIHPGPVV
                     TTFEFKPEAGVKYSKITGMTDDLSLALKAESVRIDRLPGRSTVGIEVPNRRQETIFPR
                     ELIGSPKFREYRSKLLLALGKDIHGEVYVAELDRMPHLLIAGATGAGKSVGLNGMIIS
                     MLYTSTPADLRFIMIDTKMIELGIYADIPHLLIPVVTDPKHASAALTWAVREMEQRYR
                     KLAAVGVRNIAQFNELIAEEPDRTMEHPKTGEPVPLEHLPYIVIVIDEMADLMMVSSA
                     DVEESVMRLAQMARAVGIHLILATQRPSVDVITGTIKANFPSRIAFRVSQRVDSRTII
                     DQQGAEHLLGRGDMLFLPTGSARVLRLHSGYITEHEINRVCSFLKKMAEPVYDPSVLA
                     DSDERGGTVDDGERDEMYVDAVRTVINEGKCSITLIQRRLQLGYARAARIVDMMESQG
                     VVSPGEGSKPREVLVGPEVLETIDAGR"
     gene            complement(3045..4706)
                     /locus_tag="VD788_08170"
     CDS             complement(3045..4706)
                     /locus_tag="VD788_08170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HXV76280.1"
                     /translation="MPVSNRLEIIPLGGLGEFGMNVMAYCHGHDCVVVDVGMMFPGAD
                     QPGVDLVLPDLSFLEDCGNLHGVVLTHGHEDHVGALPHLLSTHDLPVLATAHCRGLVG
                     RRLVEYDPAFTRALRPLPPDGEPLVLGPFTIRTIRAAHSIPQSKMLVIDTPAGRVLHT
                     ADFKFDTRPPDGETTDVDALRRVGEQGVLALFADSTNADRPGTTPGEAEVARGLDRVI
                     DGAPAKVVVSSFASNIQRVRVLSELAVKHGRRLALVGTSLETQVEVAERGGLIAFPPG
                     VRISPERVMELPPREVLVLATGSQGEPLSALARIALDRHRHVAIDDDDLVIHSARRIP
                     GNEKAIDRVFDRLLRLGADVITAQDAMIHASGHPSRGDLERLLELVRPRYLVPIHGEF
                     RQLKAHHDLATRRGMGRDRVLLVESGDVIELDEQSIAVVDRVRVGQVCVDASRERIDP
                     ELLRDRRRSAGEGVVVAVVALEPDGLASAGFPQIETRGFVTGATENGFLGEARQVVVD
                     SLRRADVREREDRVALKARIHSDLKRFLRRKLQRSPLIIPVVLER"
     gene            complement(4737..5552)
                     /gene="rsmA"
                     /locus_tag="VD788_08175"
     CDS             complement(4737..5552)
                     /gene="rsmA"
                     /locus_tag="VD788_08175"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXV76281.1"
                     /translation="MSRQRITARGLGQNFLVDRAAIRRIVEALAAGPGDAVVEIGPGR
                     GALTEGLIAGAGRIVAIEYDRRLAEALRRRFAETRLIVVARDVLRVELASLGPLLGRP
                     AGAPLAVAGNLPYAISKPVAMKLIRERRSVARAILMFQKEVADRLLADPGTRSYGPLT
                     VLARLTYTVERLFDLGPGAFRPRPKVDSTVTTWRPLEATLDERTERALVSVLAAAFRS
                     RRRTLRNNLLAALDDGARVDRLLGASGLDSTVRAEAIPPDGFLGLAHGWPPSL"
     gene            5655..5987
                     /gene="trxA"
                     /locus_tag="VD788_08180"
     CDS             5655..5987
                     /gene="trxA"
                     /locus_tag="VD788_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXV76282.1"
                     /translation="MTDERILILDDDSFDNEIKSIRGPILVDFWANWCEPCKTIAPWI
                     EELSHELAGKAHFAKVDVDENGDLLNRFGIMSIPTLVVFKDGKVVDQLIGAAPKEKIR
                     GLIGRHLG"
     gene            6210..7196
                     /locus_tag="VD788_08185"
     CDS             6210..7196
                     /locus_tag="VD788_08185"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXV76283.1"
                     /translation="MQQDVQSVQEWVQRESEVIDRLIAELRSVIVGQRYLLERMMIGL
                     LADGHLLLEGVPGLAKTLSVSTLAEAVHGSFQRIQFTPDLLPADLTGTLVYNQKDGNF
                     SPRKGPLFANFVLADEINRAPAKVQSALLESMQERQVTIGDTTYELPRPFLVMATQNP
                     IEQEGTYPLPEAQVDRFMLKLRVDYPSLAEEREILDRMSAARRPKAKPVVELVDLLKA
                     REAMQAIYVDDRIKDYVVRLVHATRAPAEYRLELEGLLQYGASPRATLYMVAAGRGHA
                     FLKGRGFVTPEDIKAVGADVLRHRLILSFEAEAESVTAEEVIQRVFDAVEVP"
     gene            7193..>7519
                     /locus_tag="VD788_08190"
     CDS             7193..>7519
                     /locus_tag="VD788_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXV76284.1"
                     /translation="MNPREVIRRVRRLEIRTRRLVDQSLAGSYHSVFRGRGMEFSEVR
                     EYVAGDDVRTIDWNVSARIGHPFVKKFTEERELTVVMLVDASGSGSFGTAPATKMETA
                     AEISALL"
BASE COUNT         1224 a         2540 c         2601 g         1154 t
ORIGIN      
        1 gtcttccggc acaacgacgt cgaggacctg gcgcagaagc tcgaccgggt gccggacggg
       61 atcgatcgcg tcgtgctgat cttcgacggc atcttcagca tgcgaggcga ccacgcgccg
      121 ctcgaccaca ttgcctcgct gtccgagcac cacgcgcccc gctttcgcga cggtgtggtc
      181 acggtgatgg acgattcgca cggcctcggc gcctatggcg acagcgggcg tgggacggag
      241 gaacactgcg gggcgctcgc cgacgtgctg gtcgggacct tcggcaaggc gttcggcgtc
      301 aacggcggct tcgtcgccgg gagcggcgag ttggtcgagg cgatccggca gcgcgccgac
      361 acctacatct acaccaaccc gctcggcgcc gccgactgcg ccgccgcggt cgaggcggtc
      421 aagctggtcg acggcgacgt cggccgcgag cggctcgcca ccctgcgccg gcgcacggca
      481 cggttccgcg ccggcctggc ccaactcggt ctcgaatcga tcgacggccc gcaccccgtc
      541 gtgccgctgc tggtgcgcga caccggccgg acgcggcgga ccgtcgaggg cctgttcgag
      601 cgcggagtgc tcgccgtcgg tctgaccttt ccggtcgttc ctcgcggcga cgagacgatc
      661 cggttccagg tcaacgcggc gcacaccgag cacgacatcg accaggtgct ggccgcggtc
      721 gaacaggcgt cgcgctaacg gccggcgtcg atcgtctcca gcacctcggg accgaccagc
      781 acctcccgcg gtttgctccc ctcgccgggg ctgaccacgc cctgcgactc catcatgtcg
      841 acgatccgcg ccgcgcgagc gtagccgagc tgcaggcggc gctggatcag cgtgatcgag
      901 cacttgcctt cgttgatcac cgtccgcacc gcgtcgacgt acatctcgtc gcgttcgccg
      961 tcgtcgaccg tgccgccacg ctcgtcggag tcggccagca cgctcgggtc gtataccggc
     1021 tccgccatct tcttcaggaa cgagcagacg cggttgatct cgtgctcggt gatgtatccg
     1081 ctgtgcagtc gcaatacgcg ggccgacccg gtcggcagga acagcatgtc gccgcggccg
     1141 agcaagtgct cggcgccctg ctggtcgatg atcgttcgcg agtcgacccg ctgcgacacg
     1201 cgaaaggcga tgcgcgacgg gaagttggcc ttgatcgtgc cggtgatcac atcgaccgac
     1261 gggcgttggg tggccaggat cagatggatg ccgactgcgc gcgccatctg cgccaggcgc
     1321 atcaccgatt cctcgacgtc ggccgacgag accatcatca ggtcggccat ctcgtcgatc
     1381 acgatcacga tgtaggggag atgctcgagc gggaccggct cgccggtctt cggatgctcc
     1441 atcgtgcggt ccggctcctc ggcgatcagc tcgttgaact gggcgatgtt gcgcacgccg
     1501 accgcggcga gcttgcggta gcgctgctcc atctcgcgga ccgcccaggt gagcgcggcc
     1561 gatgcgtgct tgggatcggt gacgaccggg atcaacaggt gcgggatgtc ggcatagatt
     1621 ccgagctcga tcatcttcgt gtcgatcatg atgaagcgca ggtcggccgg agtcgaggta
     1681 tagagcatgc tgatgatcat gccgttgagg ccgaccgact tgccggcacc ggtcgcgccg
     1741 gcgatcagca ggtgcggcat ccggtcgagc tcggcgacgt agacctcgcc gtggatgtcc
     1801 ttgcccagcg ccagcaacag cttcgagcga tactcgcgga acttcggcga gccgatcagc
     1861 tcgcgcggga agatcgtttc ctgccgccgg ttgggcacct cgatgccgac cgtcgatcgg
     1921 cccggcaggc ggtcgatgcg caccgactcg gctttgagcg cgagactcag atcgtcggtc
     1981 atcccggtga tcttcgagta cttgacaccg gcctccggct tgaactcgaa ggtcgtcacc
     2041 accggccccg ggtggatcgc gacgacggtg ccctcgatcg agaactcgcg gaacttgtgc
     2101 gtgatctgct cggcgcgctc gagcagctcc tgctcgtcct cccgtcgctg gttttcgggc
     2161 tccttgagca gttcgagcgg cggcagcgaa tagccacgct tcagtcgatc gagcggcagc
     2221 gaggcctgct tgggctcgac cggcgcgggc gcggccacct cggccggctc gaccgcgccg
     2281 cggggcacga gggccggtgc cggtggggcg ggagtgctcg gcgccgccgc tgccccggct
     2341 gcgccgagcg tgatcgacgg gcggcggggg gcggtctgtc gcgcatcgtc gtcgcttgcg
     2401 cgggcggcac ccgcttcgtc cgcggcgagt tcctcgcgcc actcgcgcca atagtcgccg
     2461 agccaggcga cgccacggac gcagagcgcg gcgacgatct cggcgctacg caccagcgag
     2521 acgcgaccga acaacaccgt cacgccgacg atcgtcgtta cgcagaacag cacggcgccg
     2581 aacgtgttga gcagcgcccg caacagcgag gcgaagcgcc agccgacgta gccgccgggg
     2641 tggtcgaaca cctccccgcc gtacagcatc ggtcgaaaca gcagctcggc cagcgcgacg
     2701 acggccagcg ccaacagcga aaaaccgacg atccggcccc aaccgatcgg ttgcgcgggc
     2761 ggcttcagcc ggtaccagcc ggcgaacgcc aacaggaacg gtagcgagaa cgcaccgacg
     2821 ccgaacagct gcaggtaggt ctcggccagc gtggcgccga cgcgaccgat ccagttcgcg
     2881 gtcggcaggt cgtcgggctg cagcttgaac cacgtgctgt cggcctgcga gtagctgagc
     2941 agcgcgagca gcgacacgac gccgaacagg atcaccagca tcccgagcag ctcgctgacg
     3001 tggcgcgcgt cgcgcagttt ggcgatcgac agccgcatcg cgactcaccg ctccagcacg
     3061 acggggatga tcagcggcga gcgctgcagc ttccgtcgca ggaaccgctt gaggtcggag
     3121 tggatcctgg ccttgagcgc cactcgatcc tcgcgttcgc gcacgtccgc gcggcggagc
     3181 gagtcgacga cgacctgtcg tgcctcgccg aggaagccgt tctcggtcgc tccggtcacg
     3241 aagccgcgcg tctcgatctg tggaaacccg gcgctggcca gcccgtctgg ttcgagcgcg
     3301 acgaccgcga ccaccacgcc ctcaccggcg ctgcgccggc gatcgcgcag cagttccggg
     3361 tcgatccgct cgcgcgatgc gtcgacacag acctgcccga cgcggacacg atcgacgacc
     3421 gcgatcgact gttcgtcgag ctcgatcacg tcgccggatt cgacgagcag cacgcgatcg
     3481 cgccccatcc cgcgacgcgt ggccaggtcg tggtgtgcct tcagttggcg gaactcgccg
     3541 tggatcggca ccaggtaacg cggccggacg agctcgagca gccgttcgag gtcaccgcgc
     3601 gaagggtgac ccgatgcatg gatcatcgcg tcctgggcgg tgatgacgtc ggcccccagc
     3661 cgcagcaggc ggtcgaacac ccgatcgatc gccttctcgt tgcccggaat ccgccgtgcg
     3721 gagtggatca ccaggtcgtc gtcgtcgatc gccacgtggc gatgacgatc gagggcgatg
     3781 cgcgccagcg ccgacagcgg ttcgccctgg gacccggtgg ccagcaccag cacctcccgg
     3841 ggcggaagct ccatcacccg ctcgggcgag atgcgtaccc ccggcgggaa ggcgatcagc
     3901 ccaccgcgct cggcgacctc gacctgcgtc tcgagcgagg tgccgacgag cgccagccgg
     3961 cgcccgtgct tgaccgcgag ctcggacagc acgcggaccc gttggatgtt gctggcgaac
     4021 gaactgacga cgaccttggc cggggcaccg tcgatcacgc gatcgagccc gcgcgccact
     4081 tccgcttcgc cgggagtcgt tccgggtcga tcggcgttgg tcgagtcggc gaacagcgcg
     4141 aggacgccct gttcgcccac ccggcgcagc gcgtcgacgt ccgtcgtctc gccgtccggc
     4201 ggacgggtat cgaacttgaa gtcggcggtg tggagcacgc gcccggccgg cgtgtcgatc
     4261 accagcatct tcgactgcgg gatcgagtgg gccgcgcgga tcgtgcggat cgtgaacgga
     4321 ccgaggacga gcggctctcc gtcgggtggc agcggccgga gagcgcgggt gaaggccgga
     4381 tcgtattcga ccaatcggcg gccgacgagc ccgcggcagt gcgccgtcgc aagaaccggc
     4441 aggtcgtgcg tcgagagcag atggggcaac gcgccgacgt ggtcctcgtg gccgtgggtc
     4501 aacaccacgc cgtgcaggtt gccgcagtcc tcgagaaacg aaagatcggg aaggacgaga
     4561 tcgacgccgg gctgatcggc gccgggaaac atcatgccga cgtcgaccac gacgcagtcg
     4621 tgcccgtggc agtaagccat gacgttcatc ccgaactcgc cgagcccgcc gagcgggatg
     4681 atttccaggc ggttcgagac gggcaagctc cctccggcga acggaccgcg attatatcag
     4741 agtgacggcg gccacccgtg ggccaggccg aggaagccgt ccggcgggat cgcctcggct
     4801 cgcaccgtcg agtcgagccc gctggcgccc agcagccggt cgacgcgcgc gccgtcgtcg
     4861 agcgccgcga gcagattgtt gcgcaacgtc cggcgccggc tgcgaaaggc cgcggccagc
     4921 accgagacca gcgcccgctc ggtccgctcg tcgagggtcg cttcgagcgg tcgccaggtc
     4981 gtgaccgtcg agtcgacttt cggtcgcgga cggaacgcgc ccgggccgag gtcgaacaga
     5041 cgctcgacgg tgtaggtcaa gcgcgccagc accgtcagcg gtccgtagga ccgggtgccg
     5101 ggatcggcca gcagacgatc cgcgacctcc ttctggaaca tcaggatcgc gcgggcgacc
     5161 gaccgtcgct cgcggatcaa tttcatcgcg accggcttcg agatcgcgta cggcaggttg
     5221 ccggcgacgg ccagcggcgc gccggcgggc cggccgagca acggaccgag cgaggcgagc
     5281 tcgacgcgca acacgtcacg cgcgacgacg atcagtcggg tctcggcgaa gcgccggcgg
     5341 agcgcctcgg ccagccgccg gtcgtattcg atcgcgacga tgcggcccgc gccggcgatc
     5401 agcccttcgg tcagtgcccc acgccccggt ccgatctcga ccaccgcgtc gcccgggccc
     5461 gccgcgagcg cctcgacgat ccgccggatc gccgcgcggt cgacgaggaa gttctggccc
     5521 agcccgcgcg cagtgatccg ctgccggctc accgcggggc ccgagggtgc gggaaacggc
     5581 caaatgagct acctatatcg ctcagcacgg cagatcgaac ggttcctgtc tagcagcggg
     5641 tttccggagt cgcggtgacc gacgagcgca tcctgatcct ggacgacgac agcttcgata
     5701 atgagatcaa gtcgatccgg gggcccattc tagtcgactt ctgggccaac tggtgcgagc
     5761 cgtgcaagac gatcgcccca tggatcgagg agctgtcgca cgaactggcg ggcaaggcgc
     5821 acttcgccaa ggtcgacgtc gacgagaacg gcgacctgct caaccgcttc ggcatcatga
     5881 gcatcccgac cctggtggtg ttcaaggacg gcaaggtcgt cgatcagctg atcggcgcgg
     5941 cgcccaagga gaagatccgc gggttgatcg gccggcacct gggctgacgg gtcgcccgga
     6001 ttgacaggaa tatccggcgc tcctacaatg cgtaactcgt tgcgatccaa cggagtagcg
     6061 tgggtcatgc gcgaaggtcc cgacgatatt ccctgttgtt gatcccggtt cgaccatccc
     6121 gcgcgtcgcg cgttcgtatg gatggacggc gccgcgggtc cgcacgttcg atgcgccagg
     6181 ggaaacgagt tcaggaagga agagtgaaga tgcagcagga cgtccaatcg gttcaggagt
     6241 gggttcaacg cgagtcggag gtgatcgatc ggctgatcgc cgagctccgc agcgtgatcg
     6301 tcgggcagcg ctacttgctc gagcggatga tgatcggcct cctggccgac ggccatctgt
     6361 tgctcgaagg cgtcccgggt ctagccaaga cgttgtcggt cagcaccttg gcggaggcgg
     6421 tccacggttc gttccagcgg atccagttca cccccgacct gctgccggcc gatctgaccg
     6481 ggacgttggt ctacaaccag aaggacggca atttctcacc gcgcaagggg ccgttgttcg
     6541 ccaatttcgt cctggccgac gagatcaacc gtgcgccggc caaggtccag tcggcgttgc
     6601 tcgagtcgat gcaggagcgt caggtgacga tcggcgatac gacctacgag ctgcctcggc
     6661 cgttcctcgt catggcgacg cagaatccga tcgagcagga ggggacgtac ccgctgccgg
     6721 aggcacaggt cgaccgcttc atgctcaagc tccgtgtcga ctacccgtcg ctcgccgagg
     6781 aacgggagat cctcgaccgg atgagcgcgg ccaggcgacc gaaggccaag ccggtcgtcg
     6841 agctggtcga cctgctcaag gcccgcgagg cgatgcaggc gatctacgtc gacgaccgca
     6901 tcaaggacta cgtcgttcga ctggtgcacg ccacccgtgc accggccgag taccgtctcg
     6961 agctcgaagg tctgttgcag tacggtgcgt ccccgcgcgc gacgctgtac atggtcgccg
     7021 ccggccgggg acacgcgttc ctcaagggcc gcggcttcgt cacacccgag gacatcaagg
     7081 ccgtcggcgc cgacgtgctg cgtcatcgct tgatcctctc gttcgaggcc gaggcggagt
     7141 ccgtcacggc cgaggaggtc atccagcgtg tgttcgacgc cgtggaggtc ccgtgaaccc
     7201 gcgcgaggtg attcgccggg tccgccgtct ggagatccga acacgccggc tggtcgatca
     7261 gagtctggcc ggtagctatc actcggtctt ccgcgggcga gggatggagt tctcggaggt
     7321 ccgcgaatac gttgccggcg acgacgtccg cacgatcgac tggaacgtct cggcgcggat
     7381 cggacatccg ttcgtcaaga aattcaccga ggagcgcgag ttgacggtgg tgatgctggt
     7441 cgacgccagc ggctccggct cgttcggcac ggcgcctgcg accaagatgg agaccgccgc
     7501 cgagatctcg gcgctgctc
//
LOCUS       DASPGN010000279         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_986221, whole genome shotgun sequence.
ACCESSION   DASPGN010000279 DASPGN010000000
VERSION     DASPGN010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986221"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VD788_08195"
     CDS             complement(<1..690)
                     /locus_tag="VD788_08195"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV76285.1"
                     /translation="MNGGRAEREIDGGGRLRVGVISLGCAKNLVDTEVMLGHLDRAGC
                     EFVRDPRQADVILVNTCGFIEPAREESVQAILEAAQFKKDGRLRRLVVAGCMVQRYRA
                     ELARALPEVDAFVGLDELDRVVSGVGLAPAPVRAAAGAPALRVLRSVPRSAAAVGSRP
                     SRYLYDDTTPRKTATPAWSAYIKIAEGCDHGCAFCAIPGFRGRFRSRPVQSVVREARE
                     LAGRGVAEINLI"
     gene            complement(687..2018)
                     /gene="asnS"
                     /locus_tag="VD788_08200"
     CDS             complement(687..2018)
                     /gene="asnS"
                     /locus_tag="VD788_08200"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HXV76286.1"
                     /translation="MKRDFVYVEELSRHVGESVALNGWVVQQRSSGKIKFLVLRDGTG
                     YLQCVAFVNDVSPELFAALDRLTLESSIRVRGTVRAEARAPGGHELALEELRVIHLAA
                     DDYPIQPKEHGVDFLFDRRHLYLRSRTPHAVLRVRHEVIRACRDFLNDRGFVLIDSPE
                     LTPAACEGTTTLFETDYFGDKAYLTQSGQLYLEPACMAFGRVYCFGPTFRAEKSKTRR
                     HLTEFWMVEPEVAFMQLDGLLELAEEFVSYIVARVLDRCAESLEHLERDTAKLEAIRT
                     PFPRLTYRQAAERLLSAEAQRKMTEAGAPRFTAGSDFGGFDETLLTSEFDRPVMITHW
                     PAEIKAFYMQPDADDPATARCVDMLAPEGYGEIIGGSQRIHDHDLLLERIRQHELPVE
                     AFQWYLDIRKYGTVPHSGFGMGIERCVTWICGIRHLREAIPYPRQIHRIYP"
     gene            complement(2015..>2392)
                     /gene="fusA"
                     /locus_tag="VD788_08205"
     CDS             complement(2015..>2392)
                     /gene="fusA"
                     /locus_tag="VD788_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012882.4"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXV76287.1"
                     /translation="SSEMAFKIAGSLAFQEAMKQAQPTILEPVMAVEIVVPEENMGDI
                     MGDLSSRRGKPQGMEGHGGNQVIKAQVPMSEMLDYASTLKSLTSDRGSYSMELDHYAE
                     VPAQVQQKIVADYQAAKQQEKAG"
BASE COUNT          389 a          832 c          786 g          385 t
ORIGIN      
        1 gatcaggttg atctccgcca cgccccggcc ggccagctcg cgtgcttcac ggacgaccga
       61 ctggaccggt cgcgagcgaa accgaccgcg aaagcccgga atcgcgcaga acgcgcagcc
      121 gtggtcgcag ccctcggcga tcttgatgta ggccgaccag gcgggcgtcg ccgtctttcg
      181 cggcgtggtg tcgtcgtaga gatagcgcga cggccgtgat ccgactgcgg ccgccgagcg
      241 cggcaccgac cgcaacacgc gcagcgccgg cgcgccggcc gcggcccgca ccggcgcggg
      301 tgccaacccg acaccggaga ccacgcggtc cagctcgtcg agcccgacaa aggcgtccac
      361 ctccggcagc gcgcgggcga gctcggcgcg gtagcgctgc accatgcaac cggcgacgac
      421 caggcgccgc aacctcccgt ccttcttgaa ctgcgcggcc tccaggatcg cctggaccga
      481 ttcttcgcgc gccggctcga tgaagccgca ggtgttgacc aggatcacgt cggcctggcg
      541 cgggtcgcgg acgaactcgc aaccggcgcg gtccagatgg ccgagcatga cctcggtgtc
      601 gaccaggttt ttggcgcagc cgagactgat cacgccgaca cgcaatcggc cgccgccgtc
      661 gatctcgcgt tcggcgcgcc cgccgttcac gggtagatcc tgtgaatctg tcgcgggtag
      721 gggatcgctt cgcgcaggtg ccggatcccg cagatccacg tcacgcagcg ctcgatgccc
      781 atcccgaagc cggagtgcgg caccgtgccg tacttgcgga tgtcgagata ccactggaac
      841 gcctcgaccg gcagctcgtg ctggcggatg cgttcgagca acagatcgtg gtcgtggatg
      901 cgttgcgagc cgccgatgat ctcgccgtag ccttcgggcg ccagcatgtc gacgcagcgc
      961 gcggtcgccg gatcgtcggc gtccggctgc atgtagaacg ccttgatctc ggccggccag
     1021 tgcgtgatca tcaccggccg gtcgaactcc gacgtgagca gcgtctcgtc gaagccgccg
     1081 aaatcgctcc ccgcggtgaa ccgcggtgcg ccggcctccg tcatcttccg ctgcgcctcg
     1141 gcgctcagca gccgttcggc cgcttgacga taggtcagcc gcgggaacgg cgtgcggatc
     1201 gcctccagct tggcggtatc gcgctccagg tgctcgagcg actcggcgca gcgatcgagc
     1261 acgcgggcca cgatgtagct gacgaactcc tcggcgagct cgagcagccc gtcgagctgc
     1321 atgaacgcaa cttccggctc caccatccag aactcggtca aatggcgtcg cgtcttcgac
     1381 ttctccgcgc ggaacgtcgg accgaagcag tagacgcggc cgaacgccat gcacgccggc
     1441 tcgagataga gctggcccga ctgcgtgagg taggccttgt cgccgaagta gtcggtttcg
     1501 aacagcgtcg tcgtgccctc gcacgcggcc ggggtcagct cgggcgagtc gatcagcacg
     1561 aaaccgcgat cgttgaggaa gtcgcggcag gcgcggatca cctcgtgccg gacgcgcagc
     1621 accgcgtgcg gagtgcgcga gcgcagatag aggtgccgtc ggtcgaacag gaagtcgacg
     1681 ccgtgctctt tcggctggat cggatagtcg tcggccgcca gatggatcac gcgcagttcc
     1741 tcgagcgcca actcgtgacc gcccggagcc cgggcttccg cccgcaccgt accgcggacg
     1801 cgaatcgacg attcgagcgt cagccggtcg agcgcagcga acagctcggg tgagacgtcg
     1861 ttgacgaacg ccacgcactg caggtatccg gtgccgtcgc gcagcacgag gaacttgatc
     1921 ttgcccgacg aacgctgctg gacgacccag ccgttgagcg caacgctctc gccgacgtgg
     1981 cgcgacagct cctcgacgta gacgaaatcg cgcttcaccc ggccttttcc tgctgtttgg
     2041 ccgcctggta gtcggcgacg atcttctgct gcacctgcgc cgggacttct gcgtagtggt
     2101 cgagctccat cgagtacgag ccgcggtcgc tggtcaacga cttcagtgtc gacgcgtagt
     2161 ccagcatctc cgacatcggg acctgagctt tgatcacctg attgccgccg tggccctcca
     2221 tcccctgcgg cttgccccga cgcgaggaca gatcgcccat gatgtccccc atgttctcct
     2281 cgggaacgac gatctcgacc gccatcaccg gttcgaggat cgtcggctgg gcctgcttca
     2341 tcgcctcctg gaacgcgagc gacccggcga tcttgaacgc catctccgag ga
//
LOCUS       DASPGN010000280         7764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_518474, whole genome shotgun sequence.
ACCESSION   DASPGN010000280 DASPGN010000000
VERSION     DASPGN010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7764
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518474"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..855
                     /locus_tag="VD788_08210"
     CDS             376..855
                     /locus_tag="VD788_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76288.1"
                     /translation="MFQNNAEISRRVYRALKTPSDDNPRTDPILAFHFDGRDDYIMMR
                     CNKNPEISIKGKGQTAGEVKFFAYSDFADFSEHDVLFRLKDDVRLIELYRNGAIEPIS
                     FENWTEFGKWLDGLDENGVQISLKLPYLVYQSVRGQRPSMQNFIVEAIKSALRGGTE"
     gene            852..2618
                     /locus_tag="VD788_08215"
     CDS             852..2618
                     /locus_tag="VD788_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76289.1"
                     /translation="MKRPATTRLALSLALCSVATIRLASATDAEGTPEPGSAPSPGDR
                     GMMLTAIAGGTIEEVPLTYLGVQRDLAGPGYDVHLVRLEGPVGERIGVAAGMSGSPVY
                     FGGELLGALAYRFGALPREAVGGVTPIDDILGADRARPSAAAGPGDTLVTPIATPVQI
                     SGLTGAVRRWLEPQLDELGFMVVAGGEGAPGDDAATPLVPGAPVGVALVRGDLSIAAT
                     GTVTYVDRDTVYAFGHPFLGSGSVEMPMVAVDVVHTLADLAGSVKMTRVRGQMGAIVE
                     DRLTAIVGRFGLEARMIPLELVVRGGDYGDQRFDFELARSPVLSPILAAVSVANALSA
                     NVGYRQQATMLARGEIVIRGMPRLPIEVAATSGVGADPAIGVAAAVQQVLGTLWINPF
                     SEVELDKLALEVRVDAEVRSYRVDDLHYDRGLLRPGQNLTVECVLRKYRGETVSRSFE
                     IHVPDDLPADANLALVVGSPTEVDRALGRPLARRYGSVEDVRAVIDILADLRAANRLT
                     AVLYRSSPGVVSRGAAYSDLPPSAERLMTASPGTPSSRNTTVSELFRTEVELDGPVEG
                     GVVVRLRVDSDLSDGIEAEDRP"
     gene            2615..4867
                     /locus_tag="VD788_08220"
     CDS             2615..4867
                     /locus_tag="VD788_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76290.1"
                     /translation="MRRTRSRYLLPLGLALLAGAGAVHAVSVRSWSASTQDEFARGTL
                     DGTSIDGEGRVRLAPSLVTLWGPDQGVVWSVQPDSAPDAAFVALSGPGRVLRVAAGQP
                     AEIWFEAVGETLVTAMARDPSGGVWFGLSPDGKVMRASRDGGAVVVSEVATTDALFVW
                     TVTRQRDGSLWIGTGVPGRVLRLEPGAEPKLLFDAGDDPVRSAAALPDGGVVFGTGGR
                     GRVVRVRPDGRAFVLYDADETEIVSLAAAADGTIFAVATESPKQPAARTRSATATEGG
                     VQVVTVTAPPPGEEETPGEEEQIAPAPTPRPQAFQTRPGGSLYRLDPDGTVRKLWQTD
                     REMPFAVVVATSDSLLVGTGDGGRVHALDTDGRASRLLRIPSDQVSAMAIDDAGRVVL
                     GGTTDARVESLGPGPRGEGSYLTPAVDAESTADWGRLLWEADAPRGSRLDVEVRSGNT
                     AEPDDTWSDWTAASTSGAAGEARTEVPAARWFQARFRFRAGRDESPTLRRVEVSYQPR
                     NRPPHVSHLAIEPPGVVWLRGPTQSAYRMGPVVADDPIARRIADSITAGRRGVPVDGA
                     IRKTYEAGARTIYWSAEDPDGDELTYSLAIRRDGENDWFPMTRELTDDFHSWDARAVP
                     DGIYRVRLSVDDSTDNPNGTHRRAERISDAFRVDNTPPTVGSPETTRERQRLEVRFEA
                     FDRGGSVAALEVALDGGDWAPLSPLDGVADSERERYEFEIADPGGPVTMTVRVTDSAG
                     NLGGAMWRLD"
     gene            complement(4917..6215)
                     /locus_tag="VD788_08225"
     CDS             complement(4917..6215)
                     /locus_tag="VD788_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXV76291.1"
                     /translation="MQYLDFQFLIPTSVALVLGGALLVVVMRKRRLLDEQGRLRTKLA
                     RADAQNREQSRLISRLRSEKGSVASLALSLPAVVRELNRSDIDPRRVPRLILNLAEAL
                     FQPSQFLFYIARPGSVDGTRPPELVLSTHYGFGDVPHSLRRIPFGEGKIGWVASHRID
                     MLREDWLNPARTQGDKVGDNHRSLKADIMGPLVHHSLEGEQVLGVLCIGSTGTRPSDE
                     KLMFQMVTNLGSLALVNSHLVTRLRDRANTDGLTSLLNKRYFMEELLARMIVAAEREG
                     QALALFIFDIDHFKSYNDTNGHPAGDDLLRTLAELLRRQVRPGDCCCRYGGEEFIVAM
                     PDTNREEAYSVADRIRGAVESFEFEHHESQPGGRVTISGGVAAFPHDGNSVAALTKHA
                     DVALYESKRRGRNRITLYQGVDIGTAEDVSGFDLVPVDEG"
     gene            6325..6975
                     /locus_tag="VD788_08230"
     CDS             6325..6975
                     /locus_tag="VD788_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV76292.1"
                     /translation="MSPVKLVVNEVFHSIQGESSHAGRPCVFVRLTGCNLRCVWCDTA
                     YAFHEGEETTIEAVVAEVARFGCRLVEVTGGEPLLQPAAVPLMTRLLEAGHEVLLETG
                     GSLPIEDVPEGVRRIVDVKCPRSGESERNRWQNLDHLRPGDELKFVVAGRDDYRWAIE
                     QIAGRGLAARAPILFSAVCGSLDPGELARWVLDDRIEARVQLQLHKVLWPGVTRGV"
     gene            6968..7666
                     /gene="queC"
                     /locus_tag="VD788_08235"
     CDS             6968..7666
                     /gene="queC"
                     /locus_tag="VD788_08235"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HXV76293.1"
                     /translation="MSDDVGRPAVVLLSGGLDSATTLALVLARGFDASALTVRYGQRH
                     AVEIACARAVARALGATRHHVADLDLRFVGGSALTDLAIAVPKGRDPAEIDRSIPVTY
                     VPARNLLFLSLALAWAEVLGASDLFIGVNAVDYGGYPDCRPEFVSAFERVANLATWRG
                     TDGCVIRVHAPLIGMTKAQIVAEAVRLGVDLGLTVSCYDPSPDGRSCGACDSCVLRAR
                     GFEQAGIADPALDR"
BASE COUNT         1110 a         2671 c         2772 g         1211 t
ORIGIN      
        1 cccgagacca cagaccgttc cgaggatcgc gtcggcccgt ttgctggtcg atcgagtggt
       61 cacggcacgt cctcgtgcgg atggcgtgcg agcctccgga cgcggtcccg ccggcaaccc
      121 tctcgccctg cgagtggaat ctagggtcgc ggacggacac cgggcaattt cgtgacccgc
      181 ggcttgtctc gccgatcgat atcggctacg gtatcgcccc gggtagctct gattagtcgc
      241 tggcattggg gagaggcccg ccgaggcatc gggggaatcc atccggaata ttgggccttt
      301 ggttgaggcg gactatggcc gaccgtatac tcagcctatt cgaaagccga gagacgaatt
      361 cgcggagcgt agcggatgtt ccagaacaac gctgagatct cgagacgtgt ctaccgggcg
      421 ctgaagaccc catccgacga caatccgcgc accgacccga tcctcgcgtt ccacttcgac
      481 gggcgcgacg actacatcat gatgcgctgc aacaagaacc cggagatctc catcaaggga
      541 aagggccaga ccgctggcga ggtcaagttc ttcgcatata gtgatttcgc ggacttcagc
      601 gagcacgacg tgctgttcag gctgaaggac gacgtgaggc tgatcgagct ctaccggaac
      661 ggcgcgatcg agccgatctc gttcgagaat tggaccgaat tcggcaaatg gctcgacggt
      721 ctcgacgaga acggcgtcca gatctccctc aagctgccct acctcgtcta tcagtccgtg
      781 cgcggtcagc gaccctcgat gcagaacttc atcgtcgagg cgatcaagtc cgccctgcgc
      841 ggcggcaccg agtgaagagg cccgccacca cacggctcgc gctttcgctc gcactttgct
      901 cggtcgcgac gatccgtctc gccagtgcga ccgacgcgga gggcacgccc gagccgggct
      961 ccgcgccgtc gcccggcgac cgcgggatga tgctgaccgc gatcgcgggc ggcacgatcg
     1021 aggaagtccc gctgacctac ctcggcgtgc agcgcgatct ggccggtccc ggttacgacg
     1081 tccacctcgt ccgactcgaa ggcccggtcg gcgagcggat cggggtcgcg gccgggatga
     1141 gcgggagccc ggtctacttc ggcggggagc tgctcggcgc gctggcctac cgtttcggag
     1201 cactgccgcg cgaggccgtc ggcggcgtga cgccgatcga cgacatcctc ggcgccgacc
     1261 gcgcgcgtcc gtcggcggcc gcgggccccg gcgacacgtt ggtcacgccg atcgcgacgc
     1321 cagtgcagat cagcgggctg accggtgcgg ttcgccgctg gctcgaaccg cagctcgacg
     1381 agctcggctt catggtggtc gccggtgggg agggcgcgcc cggcgacgac gctgcgaccc
     1441 cgctcgtgcc gggtgcgccg gtcggcgtgg cgctggtgcg cggcgacctg tcgatcgcgg
     1501 cgaccggcac ggtgacatac gtcgaccgcg acacggttta tgccttcggc cacccgtttc
     1561 tcggctcggg atcggtcgag atgccgatgg tcgccgtcga cgtggtgcac acgctcgccg
     1621 atctcgccgg gtcggtcaag atgactcggg tccgcggcca gatgggagcg atcgtcgagg
     1681 accggctgac cgcgatcgtc gggcgtttcg gtctcgaggc gcggatgatc ccgctcgagc
     1741 tcgtcgtccg cggtggcgac tacggcgacc agcgcttcga cttcgagctg gcgcgcagtc
     1801 ccgtcctctc gcccatcctc gccgcggtct cggtcgccaa cgcgctgtcg gcgaacgtgg
     1861 gctaccgcca gcaggcgacg atgctggcgc gaggcgagat cgtcattcgc ggcatgccgc
     1921 gcctgccgat cgaggtcgcg gcgacgagcg gcgtcggcgc ggatcccgcg atcggcgtgg
     1981 ccgccgccgt gcagcaggtc ctcggcacgc tctggatcaa cccgttcagc gaggtcgagc
     2041 tcgacaagct ggcgctcgag gtgcgtgtcg atgcggaagt gcggagctac cgggtcgacg
     2101 acctgcacta cgaccgcggc ctgctgcgac ccgggcagaa cctgacggtc gagtgcgtgt
     2161 tgcggaagta ccgtggcgag accgtctcgc gctcgttcga gatccacgtg cccgacgatc
     2221 ttccggccga cgccaacctg gcactcgtcg tcggcagccc gaccgaggtc gaccgcgcgc
     2281 tcggccggcc gctcgcgcga cgctacggca gcgtcgaaga cgtgcgcgcc gtgatcgaca
     2341 tcctggccga cctccgagcg gccaaccggc tgacggcggt gctctaccgc agctcgcccg
     2401 gcgtcgtctc gcgcggcgcg gcgtactccg atctgccgcc gagcgccgag cggttgatga
     2461 ccgcgagccc ggggacgccg agcagcagaa acaccaccgt ctccgagctg tttcgcacgg
     2521 aggtcgagct cgacggtccg gtcgagggtg gggtcgtcgt ccgcttgcgc gtcgactcgg
     2581 acctcagcga cgggatcgag gcggaggacc gtccatgagg cggacgcgtt cccggtatct
     2641 cctcccgctc ggtctcgcgc tgctcgccgg ggcaggcgcg gtccacgcgg tctccgtacg
     2701 gtcgtggtcg gcgtcgaccc aggacgagtt cgcccgcggc acgctcgacg gtacgtcgat
     2761 cgacggcgag ggacgggtgc gactggctcc gtccctcgtg acgctgtggg ggccggacca
     2821 gggtgtcgtg tggagcgtcc agcccgactc cgcgcccgac gcggcgttcg tcgcgctgtc
     2881 gggacccggc cgcgtgctgc gtgtcgcggc cgggcaaccg gccgagatct ggttcgaggc
     2941 cgtcggcgag acgctggtga cggcgatggc acgcgacccg agcggcggcg tctggttcgg
     3001 gctgtcgccc gacggaaagg tgatgcgggc ctcgcgcgac ggcggtgcgg tcgtcgtcag
     3061 cgaggtggcg acgaccgacg cgctgttcgt ctggaccgtg acgcgccaac gggacgggtc
     3121 gctgtggatc ggcaccggcg ttcccggccg cgtgctgcgt ctcgagcccg gagccgagcc
     3181 gaaactgctg ttcgacgccg gcgacgaccc cgtgcgcagc gccgccgcgc tccccgacgg
     3241 gggcgtggtg ttcggcacgg gcggccgagg ccgggtggtc cgcgttcgcc ccgacggtcg
     3301 ggcattcgtc ctgtacgacg cggacgagac ggagatcgtc tcgctcgcgg ccgccgccga
     3361 cggaacgatc ttcgccgtcg cgaccgaatc gcccaaacaa ccggcagcac gcacacgaag
     3421 cgcgacggca accgagggcg gcgtgcaggt cgtaaccgtc accgcgcccc cccccggcga
     3481 ggaggagacg cccggcgagg aggagcagat cgccccggcg ccgacgcccc ggccccaggc
     3541 gttccagacg cgacccggcg gatcgctcta ccgcctcgac cccgacggca ccgtccgcaa
     3601 gctctggcag accgaccgcg agatgccgtt cgccgtcgtc gtcgcgacga gcgactcgtt
     3661 gctggtcggc accggcgacg gaggccgggt ccacgcgctc gacaccgacg gtcgggcgtc
     3721 gcggctgttg cgtattccgt cggaccaggt gagcgcgatg gcgatcgacg acgccggtcg
     3781 cgtcgttctc ggtggtacga ccgacgcacg cgtcgagtcg ttgggtccgg ggccacgcgg
     3841 ggaggggagc tacctcactc ccgcggtcga cgccgaaagc accgccgact ggggacggct
     3901 gctgtgggaa gcggatgctc cgcgcggcag ccggctcgat gtcgaggtcc gctcgggcaa
     3961 caccgcggag cccgacgaca catggtcgga ttggaccgca gcctccacgt ccggcgcggc
     4021 cggggaggcc cgcaccgagg tcccggccgc ccggtggttc caggcccgct ttcgcttccg
     4081 cgccggccgg gacgagtcgc cgacgctgcg gcgcgtcgag gtctcctacc agccgcgcaa
     4141 ccggccaccc cacgtttccc acttggcgat cgagcccccg ggcgtcgtct ggttgcgcgg
     4201 accgacccag tccgcgtacc ggatgggacc ggtggtcgcg gacgatccga tcgcccggcg
     4261 catcgccgac tcgatcacgg ccggtcgccg cggcgtcccg gtggacgggg cgatccgcaa
     4321 gacctacgag gccggcgccc ggacgatcta ctggtctgcc gaagacccgg acggcgacga
     4381 gttgacctac tcgctggcga tccggcggga cggggagaac gactggttcc cgatgacgcg
     4441 cgagctgacc gacgacttcc acagctggga cgcgcgggcg gtccccgacg gcatctatcg
     4501 cgtacggctg tcggtcgacg attcgaccga caacccgaac ggcacgcacc ggcgtgccga
     4561 gcggatcagt gacgcgttcc gcgtcgacaa tacgccgccc acggtcggtt cgccggaaac
     4621 cacccgcgag cggcaacggc tcgaggttcg cttcgaggcc ttcgaccgcg gcggtagcgt
     4681 ggcggcgctc gaggtcgcgc tcgacggcgg cgactgggcc ccgctcagcc cgctcgacgg
     4741 cgtggccgac tccgagcgcg agcgctacga gttcgagatc gccgaccccg gcgggccggt
     4801 gacgatgacc gtgcgggtca ccgactcggc cggcaacctc ggcggggcga tgtggcggct
     4861 cgactgacgc gtgtgagccg gtcggtcgag atcgaagcgg tgcgtcgaat cgcgtactag
     4921 ccctcgtcga ccggtaccag atcgaagccg ctcacgtcct cggccgtgcc gatgtccaca
     4981 ccttggtaga gcgtgatgcg gttcctgccg cgccgtttcg actcgtacag cgcgacgtcg
     5041 gcgtgcttgg tcagtgccgc gacgctgttg ccgtcgtgcg gaaaggcggc gacgccgccg
     5101 ctgatcgtga cccgtccccc gggctggctc tcgtggtgct cgaactcgaa cgactcgacg
     5161 gcgccacgga tgcggtcggc gaccgagtag gcctcctcgc gattcgtgtc gggcatcgcg
     5221 acgatgaact cttctccacc gtagcggcag cagcagtcac cgggacgaac ctggcgccgc
     5281 agcagctcgg ccagcgtgcg gagcaggtcg tcgcccgccg ggtggccgtt ggtgtcgttg
     5341 tagctcttga agtgatcgat gtcgaagatg aacagcgcca gcgcctgacc ctcgcgctcg
     5401 gcggccacga tcatccgcgc cagcagctct tccatgaagt agcgcttgtt gagcagcgag
     5461 gtcagcccgt cggtgttcgc gcggtcgcgc agccgggtga cgaggtgcga gttgacgagc
     5521 gcgagcgagc cgaggttggt gaccatctgg aacatcagct tctcgtcgct cggccgcgtt
     5581 ccggtcgagc cgatgcacag cacgccgagc acctgctcgc cttcgagcga gtgatgcacg
     5641 agcggtccca tgatgtccgc cttcagcgag cgatggttgt cgcctacctt gtcgccctgc
     5701 gtacgcgcgg ggttgagcca gtcctcgcgc agcatgtcga tccggtgcga ggcgacccag
     5761 ccgatcttgc cttcgccgaa cgggatccgt cgcagcgaat gggggacgtc gccgaagccg
     5821 tagtgcgtgc tgagcacgag ctccggcgga cgcgtgccgt cgaccgagcc ggggcgcgcg
     5881 atgtagaaca ggaactggct gggctgaaac agcgcctcgg ccaggttgag gatcaggcgc
     5941 ggcacgcgcc gcgggtcgat gtcgctgcga ttcagctcgc gcacgaccgc cggcagcgag
     6001 agcgccaggc tggcgaccga tcctttctcg ctgcgcagtc tcgagatcag ccgactctgt
     6061 tcgcgattct gggcgtcggc gcgggccaac ttggtccgca accgcccctg ctcgtccagg
     6121 agacggcgtt ttcgcatcac gacgaccagc agcgctccgc cgagcaccag cgcgacactg
     6181 gtcgggatca ggaactggaa atccaggtac tgcactgcaa cccttcgtcg gccgggctcc
     6241 cgcggacaga cgtcccgcga gaccccggcg cgtgcgggcg cccggcgagg tggtccaccg
     6301 cgctccccgg cactcgtgtg taaaatgagt cccgtgaagc tcgtcgtcaa cgaggtcttc
     6361 cattccatcc agggggaatc gtcacatgcg gggcggccgt gcgtcttcgt acggctgacc
     6421 ggatgcaacc tgcgatgtgt ctggtgtgac acggcttacg ccttccacga gggcgaggaa
     6481 acgacgatcg aagcggtcgt tgccgaggtg gcgcgcttcg gctgccggct ggtggaggtg
     6541 acgggcggcg agccgctgct gcaaccggcg gccgtgccgt tgatgacccg gctgctcgag
     6601 gccggccacg aggtgctgct cgaaaccggt ggtagcttgc cgatcgagga cgtgcccgag
     6661 ggggtgcggc ggatcgtcga tgtgaagtgt ccgagaagcg gcgagtcgga gcggaatcgc
     6721 tggcagaatc tcgaccacct gcggccggga gacgagctga agttcgtcgt cgccggccgc
     6781 gacgactacc gttgggcgat cgagcagatc gcgggccggg ggctcgcggc ccgggcgccg
     6841 atcctgttct cggcggtctg cggttcgctc gatccgggcg agctggcgcg ctgggtgctc
     6901 gacgatcgga tcgaggcccg cgtccagctc cagttgcaca aggtcctgtg gcccggtgtc
     6961 acgcgcggtg tctgacgacg tcggtagacc ggccgtcgtg ctcctgtcgg gcggtctgga
     7021 ttcggccacg accctggccc tggtcctcgc ccgcggcttc gacgccagcg ccttgacggt
     7081 gcgctacggt cagcgtcacg ccgtcgaaat cgcctgcgcg cgggcggtgg cgcgcgcgct
     7141 gggcgccacg cgtcaccacg tcgccgacct cgatctgcgc ttcgtcggcg gctcggcctt
     7201 gaccgacctc gcgatcgccg tgccgaaggg acgcgatccg gccgagatcg atcggtcgat
     7261 tcccgtcacc tacgtccccg cgcgcaacct gctcttccta tcgctcgccc tcgcctgggc
     7321 cgaggtgctc ggcgcgtcgg acctgttcat cggggtcaac gcggtcgact acggtgggta
     7381 cccggactgc cggccggagt tcgtctccgc cttcgagcgc gtcgccaatc tggcgacgtg
     7441 gcgcggcacc gacggctgcg tgatccgcgt ccacgcgccg ctgatcggca tgacgaaggc
     7501 gcaaatcgtc gccgaggccg ttcgactcgg cgtcgacctc ggtctgaccg tctcgtgcta
     7561 cgacccgtcg cccgacggtc ggtcgtgcgg tgcgtgcgac tcgtgcgtcc tgcgtgcccg
     7621 aggcttcgaa caggcgggca tcgccgaccc cgcgctcgac cgctgattcg ccggccggac
     7681 atccatcgtt gccccggcgg ggctcggagc gctaggatgc tccccgccgg gggcgtgccc
     7741 cgtggaggac agatccgcat ggag
//
LOCUS       DASPGN010000281         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1337131, whole genome shotgun sequence.
ACCESSION   DASPGN010000281 DASPGN010000000
VERSION     DASPGN010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337131"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1930)
                     /locus_tag="VD788_08240"
     CDS             complement(<1..1930)
                     /locus_tag="VD788_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXV76294.1"
                     /translation="MSTHDRDTRESPWELAVGPPPDRWDDWVELDAAAWPRRVERHYR
                     CIPTICFNCESACGLLAFVDKRTGEIRKFEGNPVHPGSRGRTCAKGPATINQIRDPER
                     ILYPLKRSGPRGAGRFERVSWDEALDEIGERVRRALDDGRHDEVIYHVGRPGDDHFMP
                     RLLGAWGIDGHNSHTNVCSSSARAGYALWLGSDRPSPDYAHSRFILLLSAHLETGHYF
                     NPHAQRIIEAKNSGARMAVVDTRLSNTSSHADLWISTWPGSEAALLLGVARRLLESGR
                     FDAEFVRRWTNWPAYLVACDPGGPGTFARFVEHLTARYARFTPTFVAEECRIDGAIVD
                     RLADEIAAAGSRFASHLWRNAASGNLGGWQIARALMLLHVLSGSIGTPGGVDPNDWDK
                     AVPKPSENPPASGRWNELLWPRQFPLAHYEMSFLLPYLQRDRRRGLEVYFTRVYNPVW
                     TNPDGCAWIEMLEDPRRVGCHVALTPIWSETAQWADFVLPMGLGPERHDLMSQETHAG
                     SWIGFRQPVSREFARLEGRPVHDTRQTNPGEVWEEAEFWIALSWRIDADGSRGIRRYY
                     ESRRSPGSPVTLDEYYADIFAGVPGLAQAARAAGTTPLDYMRRRGAFAVPYAGQRRYE
                     KRADDGPAGFPTPSRRLEL"
BASE COUNT          294 a          708 c          717 g          299 t
ORIGIN      
        1 acagctcgag ccgccggctg ggggtgggaa aaccggccgg gccgtcgtcg gcccgcttct
       61 cgtaccggcg ctgaccggcg tagggcacgg cgaacgcccc gcggcgtcgc atgtagtcga
      121 gcggcgtcgt tccggcggcc cgcgccgcct gggccagccc cggcacaccg gcgaagatgt
      181 ccgcgtagta ctcgtcgagc gtcaccggcg agccgggcga tcgcctcgac tcgtagtagc
      241 gacggatgcc gcgcgagccg tcggcgtcga tgcgccagct cagcgcgatc cagaactcgg
      301 cttcctccca cacttcaccc gggttggttt gccgggtgtc gtggacgggc cggccctcga
      361 gacgcgcgaa ctcgcgcgag accggctggc ggaatccgat ccagctgccc gcgtgggtct
      421 cctggctcat caggtcgtga cgctccggac cgagacccat cggcaggacg aaatcggccc
      481 attgcgccgt ctcggaccag atcggggtca gtgcgacgtg acagccgacg cggcgggggt
      541 cctcgagcat ctcgatccag gcgcagccgt cgggattggt ccacaccggg ttgtagaccc
      601 gggtgaagta gacctcgagc ccgcgccgcc ggtcccgctg caggtagggc aacaggaagc
      661 tcatctcgta gtgcgcgagc ggaaactggc gcggccagag caactcgttc cagcggcccg
      721 aggccggcgg gttctcgctc ggtttgggga cggccttgtc ccagtcgttc gggtcgactc
      781 cgccgggcgt gccgatgctc ccgctcagca cgtggagcag catcagcgca cgcgcgatct
      841 gccagccgcc gagattgccg ctggccgcgt tccgccacag gtggctggcg aagcggctgc
      901 cggccgcggc gatctcgtca gccaaacggt cgacgatcgc cccgtcgatc cggcattcct
      961 cggcgacgaa ggtcggcgtg aaccgcgcgt agcgcgcggt caggtgctcg acgaaccgcg
     1021 cgaaggtccc gggcccgccg gggtcgcagg ccaccaggta ggccggccag ttggtccagc
     1081 gccggacgaa ctcggcgtcg aaccgcccgc tctcgagcag gcgccgcgcg acgccgagca
     1141 acagcgcggc ctcgctgccc ggccaggtcg agatccacag gtcggcgtgg gacgaggtgt
     1201 tgctcaagcg ggtgtcgacg accgccatgc gggcgcccga gttcttggcc tcgatgatcc
     1261 gctgggcgtg cgggttgaag tagtgccccg tctcgagatg ggccgacagc agcaggatga
     1321 aacgcgagtg ggcgtagtcc ggcgacggac ggtccgaacc gagccacagc gcgtagcccg
     1381 cgcgggccga cgagctgcag acgttcgtgt ggctgttgtg cccgtcgatg ccccacgcgc
     1441 cgagtagacg gggcatgaag tgatcgtcac ccggtcgccc gacgtggtag atcacctcgt
     1501 cgtgccgccc gtcgtcgagc gcgcgtcgca cgcgctcgcc gatctcgtcg agcgcctcgt
     1561 cccaggagac acgctcgaac cgacccgcgc cgcggggtcc gctgcgcttg agcgggtaga
     1621 ggatgcgctc cggatcgcga atctggttga tcgtcgccgg tcccttggca caggtgcgcc
     1681 cgcggctccc cgggtgcacc gggttgccct cgaacttgcg gatctccccg gtccgcttgt
     1741 cgacgaaggc cagcagaccg caggccgact cgcagttgaa acagatcgtc gggatgcagc
     1801 ggtagtgacg ctcgacccga cgcggccagg ctgccgcgtc gagctcgacc cagtcgtccc
     1861 agcggtcggg gggcgggccg acggcgagct cccacggcga ctctctcgta tcccgatcgt
     1921 gcgtgctcat cgtttcgccc tcaactgatc gccggggcct gcccggcgcg cacgagtatc
     1981 tcctgctcgc acagcatccc cgcgagcgcg agtaccga
//
LOCUS       DASPGN010000282         4703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1454631, whole genome shotgun sequence.
ACCESSION   DASPGN010000282 DASPGN010000000
VERSION     DASPGN010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454631"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /gene="miaB"
                     /locus_tag="VD788_08245"
     CDS             complement(<1..1081)
                     /gene="miaB"
                     /locus_tag="VD788_08245"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXV76295.1"
                     /translation="MGDRGWAPDAVDRAGNCPRAMSRYHIETWGCQMNVHDTEKLAGL
                     LEQRGMERAAGRGDADLILLNTCSIREKAAEKMFSELGRIERLRRKNPDLLIGVCGCV
                     GQQEGQAVFARAPYVDFVIGPRATGSLPGVLDRLRRGDPTARRFVDVDYRDDSIRFPY
                     DAIRRESPETAKAYVTIIEGCNHRCTYCIVPTTRGREICRAMAEVLAEVRALARSGVL
                     EVEFLGQTVNAYRDDRGQTLADLLRATAAIDGIDRIRFTTSHPAQMTERLMDAMRDAR
                     PALCPYLHLPVQSGSSAVLERMRRGYDRETYLDKIEEIRRRIPEISFGTDIIVGFPGE
                     TDEEFDDTLRLLEEVPFDTVYSFAYS"
     gene            complement(1107..1475)
                     /locus_tag="VD788_08250"
     CDS             complement(1107..1475)
                     /locus_tag="VD788_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXV76296.1"
                     /translation="MTKADLVDEVVRVTNVSKKHAEIIVNTVFSSIIEALKGEDKIEL
                     RGFGSFRIRRRRPRQGRNPKTGDQVAVPEKRIPYFKPGKELKDLINEDGASSVARTPP
                     LARDSSVATSRDPRFDGGRE"
     gene            complement(1529..3403)
                     /locus_tag="VD788_08255"
     CDS             complement(1529..3403)
                     /locus_tag="VD788_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXV76297.1"
                     /translation="MDTQQETPDTKKADPTDSLGESADAEPTGEQANGSAASVSLGID
                     VEEVDDFGQALADFESGAKTIKEGEVVRGRVLKLLEKEVIVDIGYKSEGVIDLEEFRG
                     VDGQIHVAPGDRVDVLLEKTEDSDGYVVLSKEKAERLKVWDSVEKAYETGEPLMGRVL
                     DRIKGGLKVDIGLPAFLPGSLVDVRPIRNLESLIGHEYRMRVIKVNKRRGNIVLSRKA
                     VLEIENAEKKEKTLADLEEGKILRGVVKNLTEYGAFIDLGGIDGLLHITDISWGRINH
                     PSEKFQIGDELDVIVLKFDRERERVSLGFKQLQDDPWENAPDKYPPHSRIRGKVVSLT
                     DYGAFVEVEEGIEGLIHVSEMSWTKRVKHPSKMLNVGDWVECVVLEIDQEGRRLSLGL
                     KQTEPNPWDLISSKYRIGDKITGTVRNITDFGAFIEVEEGIDGLVHISDLSWTQRVKH
                     PGEVLEKGAEVEAVILKIDSDNQRLSLGIKQLQPNVLEEFVQTHGTGDVLTGKIVRLT
                     EFGAFVELFEGVEGLVHVSELANERIERPEDKFSVGQDVRVKIIKMDPVEKKIGLSIR
                     AALNEPDSAAVEAYFNNQGDGSATLGDLMDPSMFRQKQGTDDERPEADTPDRSDDDEE
                     "
     gene            complement(3568..4314)
                     /locus_tag="VD788_08260"
     CDS             complement(3568..4314)
                     /locus_tag="VD788_08260"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXV76298.1"
                     /translation="MRLNKALAHAGLASRRGADRLIAEGRVTVNGETVADLGRQVDPL
                     HDAIKVDGRRLPAPPPAHTYLALHKPRGYMTTRSDPENRPTVMQLVSGVKARVYPVGR
                     LDFLTEGLLLLTDDGQLARDLTHPASEVPKTYRAKVHGRPEAAELDRLRRGIRLDGDL
                     TTPAKVQLTKGGPNSWVEIVITEGRKHQVRRMLAAVGHPVLKLKRTRFDGIDLGSLPV
                     GASRPLSPAEIERLRRSAGGKAGPRADRRN"
     gene            complement(4320..>4703)
                     /locus_tag="VD788_08265"
     CDS             complement(4320..>4703)
                     /locus_tag="VD788_08265"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXV76299.1"
                     /translation="LAIVAYRQPITAPEIQAIRGKDPSAALKTLLEKKLLRILGRKKV
                     VGNPLLYGTSKQFLIHFGLNSLSDLPAIEDFEELVEALAAEQPELFAADAEEEGEGDT
                     SDGPPWPGESDEWAESVDGETRVDH"
BASE COUNT          709 a         1587 c         1513 g          894 t
ORIGIN      
        1 ccgagtaggc gaacgaatag accgtgtcga acgggacctc ttcgagcaat cgcagcgtgt
       61 cgtcgaactc ctcgtcggtc tcgccgggaa agccgacgat gatgtccgta ccgaacgaga
      121 tttcgggtat ccgccgccgg atctcctcga tcttgtcgag gtacgtctcc cggtcgtagc
      181 cgcggcgcat ccgctcgagg acggccgagc tacccgactg caccggaagg tgcaggtacg
      241 gacacagcgc cggtcgagca tcccgcatcg cgtccatcaa ccgctcggtc atctgcgccg
      301 ggtgcgacgt ggtgaagcgg atgcgatcga tgccatcgat cgcggcggtg gcgcgcagca
      361 gatcggcgag ggtctggcct cggtcgtcgc ggtaggcgtt gacggtctgg ccgaggaact
      421 cgacctcgag cacgccggac cgagccagcg cacgcacctc ggccaacact tcggccatcg
      481 cgcggcagat ctcgcgaccg cgcgtggtcg gcacgatgca gtacgtgcac cgatgattgc
      541 agccctcgat gatcgtgaca taggccttgg cggtttcggg actctcacgg cggatcgcgt
      601 cgtacgggaa tcggatcgag tcgtcgcggt agtcgacgtc gacgaagcgc cgcgcggtcg
      661 gatcgccgcg gcgcagccga tcgagcacgc cgggcagcga gccggtcgcg cgcggtccga
      721 tgacgaagtc gacgtacggc gcacgggcga agaccgcctg cccttcctgc tgtccgacgc
      781 agccgcagac gccgatcagc agatcggggt tcttgcgccg caagcgctcg atgcgaccca
      841 gctcgctgaa catcttctcc gcggccttct cgcgaatcga gcaggtgttg agcaggatga
      901 ggtccgcgtc gcctcgtccg gcggcacgct ccatcccgcg ctgctcgagc aggccggcga
      961 gcttctccgt gtcgtggacg ttcatctggc agccccaggt ctcgatgtgg tagcggctca
     1021 tcgcccgcgg gcagttgccc gcgcggtcca cggcatcggg agcccacccg cggtcgccca
     1081 tcacggtcag cggggtgcgc tcggggtcat tcgcggcctc cgtcgaaccg cgggtcgcgg
     1141 gaggtggcca cggacgaatc acgggcgagc ggcggggtgc gtgcgacgct cgacgcgccg
     1201 tcctcgttga tcaggtcctt cagttctttc ccgggcttga agtagggaat ccgtttttcc
     1261 ggcacggcga cctggtcgcc ggtcttcggg ttgcgtcctt gccgcggccg ccggcgccgg
     1321 atgcgaaagc tgccgaaccc ccggagctcg atcttgtcct cgcctttcag cgcctcgatg
     1381 atcgacgaga agacggtgtt gacgatgatc tccgcgtgct tcttgctgac gttcgtcacc
     1441 cggacaactt cgtcaaccaa gtcggccttg gtcatccgcc accctccccg tcgaatctcc
     1501 cgtcgcggcc ggcgtcacgg acgaacgatc attcctcgtc gtcgtcggaa cggtcgggag
     1561 tgtcggcctc cggccgctcg tcgtcggtgc cctgcttctg gcggaacatg ctcgggtcca
     1621 tcaggtcgcc gagcgtcgcg ctcccgtccc cttggttgtt gaagtaggcc tcgaccgcgg
     1681 ccgagtcggg ctcgttgagg gctgcgcgga tcgacagacc gatcttcttt tcgacggggt
     1741 ccatcttgat gatcttgacg cggacgtcct gcccgacgga gaacttgtcc tcgggcctct
     1801 cgatccgctc gttggccagc tcggaaacgt ggaccagccc ctcgacgccc tcgaacagct
     1861 cgacgaaggc gccgaactcg gtcagccgga cgatcttccc cgtcagcacg tcgccggtac
     1921 cgtgggtctg gacgaattcc tcgagcacgt tgggctggag ctgcttgatg ccgagcgaca
     1981 ggcgctggtt gtccgagtcg atcttgagga tcacggcttc gacttcggcc cccttctcca
     2041 gcacctcgcc ggggtgcttg acccgctggg tccacgacag atcggagatg tggaccaggc
     2101 catcgatccc ctcttcgacc tcgatgaacg cgccgaaatc cgtgatgttg cgcacggttc
     2161 cggtgatctt gtccccgatg cggtacttcg agctgatcaa gtcccacgga ttgggctcgg
     2221 tctgcttgag accgagcgac aggcgtcggc cctcctggtc gatctcgagc accacgcatt
     2281 cgacccagtc gccgacgttc agcatcttcg acgggtgctt gacccgcttg gtccacgaca
     2341 tttcggagac gtggatcagg ccctcgatgc cctcctcgac ctcgacgaac gcgccgtagt
     2401 cggtcaggct gacgaccttg ccgcggatgc gggaatgcgg cgggtacttg tccggcgcat
     2461 tctcccacgg gtcgtcctgt agctgtttga agccgagcga gacgcgttcg cgctcccgat
     2521 cgaacttgag cacgatcacg tcgagctcgt cgccgatctg gaacttctcc gagggatgat
     2581 tgatgcgacc ccacgagatg tcggtgatgt gcagcaagcc gtcgatgcca ccgagatcga
     2641 tgaacgcgcc gtactcggtc aggttcttga ccacaccgcg gaggatcttg ccctcctcga
     2701 ggtcggcgag cgtcttctct ttcttctccg cgttctcgat ctcgagcacc gctttgcgcg
     2761 acaacacgat gttcccgcgc cgcttgttga ccttgatcac gcgcatgcgg tactcgtggc
     2821 cgatcaggct ctcgagattg cggatcggcc ggacgtcgac caaggagccg ggtaggaagg
     2881 caggcagccc gatgtcgacc ttcagtccgc ccttgatgcg gtcgagcacg cggcccatca
     2941 gcggttcgcc ggtctcgtag gccttctcga ccgagtccca gaccttcagc cgctcggcct
     3001 tctccttcga cagcacgaca tagccgtcgc tgtcttccgt cttctcgagc aagacgtcga
     3061 cgcgatcgcc cggcgcgacg tggatctgac cgtcgacgcc gcggaactcc tcgaggtcga
     3121 tcacgccctc ggacttgtag ccgatgtcga cgatcacctc cttttcgagc agtttcagca
     3181 cgcggccgcg gacgacctcg ccctccttga tggtcttggc acccgactcg aagtcggcca
     3241 gcgcctggcc gaaatcgtcg acctcctcga cgtcgatccc caggctcacc gaggccgcag
     3301 acccgttcgc ctgctcgcct gtcggctcgg cgtcggcgga ctcaccaagc gagtcggtcg
     3361 gatcggcctt tttcgtatcg ggcgtttctt gctgcgtatc catgggattc ggtctcaacc
     3421 tcctgaacat gggcgtcgcc gcggccgccc ccgcatgtcg aaatccggtc ggtcttcacc
     3481 cgcgctgccg cgacgagtca cggcatgaaa caagtgcacc atgctagaga acgcaccggg
     3541 ccatgtcaag aacggtcgcg ttttcagtca attacgtcga tcggcacgcg gtccggcctt
     3601 tccgccggcc gagcggcgaa ggcgctcgat ctcggccggg ctgagcggcc ggctcgcgcc
     3661 gacggggagg ctgcccaggt cgatgccgtc gaatcgcgtg cgcttgagct tgagcacggg
     3721 gtggccgacg gcggcgagca ttcgccggac ctgatgcttg cgtccctcgg tgatcacgat
     3781 ctcgacccac gagttgggcc cgcccttcgt cagttggacc ttggccgggg tggtcaggtc
     3841 gccgtccaga cggattccac gccgcaggcg gtcgagctcg gccgcctccg gtcgaccatg
     3901 caccttggcg cggtaggttt tcggtacctc gcttgcggga tgcgtcaaat cccgcgcgag
     3961 ttggccgtcg tcggtcaaca gcagcagccc ctcggtgagg aagtccaacc gaccgaccgg
     4021 atagacccgg gccttgacgc cggagaccag ttgcatgacg gtcggtcggt tctcggggtc
     4081 cgagcgggtc gtcatgtagc cgcgtggctt gtggagggcc agataggtgt gcgccggagg
     4141 cggtgcgggc agacgcctgc cgtcgacttt gatcgcgtcg tgcagcggat cgacctgccg
     4201 gcccagatcc gccaccgtct cgccgttcac ggtcacgcgg ccctcggcga tcagccgatc
     4261 cgccccgcgc cgggaggcca gcccggcatg cgccagcgcc ttgttgagcc gcatcttgct
     4321 caatgatcga cccgcgtctc gccgtcgacg gattcggccc actcgtcgga ttcaccgggc
     4381 cacggcgggc cgtcgctcgt gtcgccttcg ccttcctcct cggcgtcggc ggcgaacagc
     4441 tccggttgct ccgccgccaa tgcttcgaca agctcctcga agtcctcgat cgcggggagg
     4501 tcggacaggc tgttcagacc gaagtggatc aggaactgct tcgacgtgcc gtagagcaac
     4561 ggattgccga cgaccttctt gcgaccgaga atccgcaaca gcttcttctc gagcagcgtc
     4621 ttcaacgccg cgctcgggtc cttgccgcgg atcgcctgga tttcgggagc ggtgatcggt
     4681 tgccggtagg ccacgatcgc gag
//
LOCUS       DASPGN010000283         8233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1572526, whole genome shotgun sequence.
ACCESSION   DASPGN010000283 DASPGN010000000
VERSION     DASPGN010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572526"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VD788_08270"
     CDS             complement(<1..317)
                     /locus_tag="VD788_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76300.1"
                     /translation="MILHWAEMDVLHIRSARRELDAVLRATPSKSATHRALVAAALAV
                     GPSSLLDPLDADDTRVTLDGLRALGIPIEDDGDRWRVEGRGGAVAGGATIALRQSGTS
                     MRFL"
     gene            498..1430
                     /locus_tag="VD788_08275"
     CDS             498..1430
                     /locus_tag="VD788_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76301.1"
                     /translation="MKFAADMAARGNWREASFRWEKLLTSTGGNAKLYNNLAVASEAV
                     GRLERAGELYSQALDEATGDPLIADNYRRYSRLVALLLERDPDLSDELDAPEIDVDDQ
                     GDKAKGRSARVGLNVPIPPRIDLDGIQSILVTSFRANESGFIDVNRELARFLRGEFRQ
                     RTALEVLDVVPPPAIPEQRLEDLAANSEFWKYLAREYGADLIVSGIVNFGRVEVSGFE
                     DVDVINPRTGQKMRQSQFVEKEEFNYQVDLLFIDGSTGSPLHRDRLTRKVLFRGLSND
                     PLHAFYELSESLGDDIVSVVSTRMRAESRVIFKR"
     gene            1453..4467
                     /locus_tag="VD788_08280"
     CDS             1453..4467
                     /locus_tag="VD788_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXV76302.1"
                     /translation="MRQIDVRKSLRWSWLLAAVLLASSADAQYFGQNKIAYANFDWQV
                     YKSPHFDVYYYPETEPFLEDIVSYAESAYVHLSRTLDHELRFRVPLITYKTHGEFQQT
                     NITLAELPDGVGAFAEPVQYRMVLPIDLPPDELYALIAHELTHIFEYSYFYDGYLGRA
                     IRGRAPTWLMEGLASYLGDDETNLDRMAIRDAVVNNILPPIESLNVVTFLTYRYGHAI
                     FDYIEQEHGIEGVRTFLFEFKKVLLTGNLSKAIEESLGYDIDEFNRRFNRYLRQKYFP
                     VLLEKKSPDDYGTELGTRKLRGVFTFSPALSPSGELVAALGVPGMELDLVVLSAEDGT
                     LVKNLTKGWTNKYRNLVAEVFKGKRDLSWSPREDHVAVFVRRENRWPLFIFDALSGNI
                     VDQIAFDDIYQNASPAFSTDGRRIAFEGNRNGIVDIFEVDLDTREVRNLTQDDFFDAN
                     PWYGPDGKTLLYNRRIGEHWKVFSVDLEDSSKKTQLTFGPYSELQPSYSRDGNTIYYA
                     SDRGEYGVFNLFSLDLQTGDLRQYTDVVGGCFSPVEAGQVGDETFLVFTAYYRGMFRL
                     YRMPLRAPEATIGMAQRLEEPTEAEPFEPDMRLRTDEDQKARYKLKWDIEAPDVGVGV
                     ANDGTFLANAAISFSDLMGDHRVTLAIESVSSFQSYWAQYTNIKRRGNWGARVYDVRD
                     FIVSQVDGSRLEQGSRATGADLFYEYPISRYYRLDFSVGVADTSYLTVEGFDAVSGEA
                     IFGNIEDTLGIVSADLVGDTTRYQRWGPFQGKRFRIGAEYGPRLGGDRSGDMIRYNVD
                     FRAYKQVTRRSVLAFRTAGVIGAGEIQNSYSFGGINQLRGYEYREFTGSRIAWMNLEF
                     RFPLVDEMLFPILALQDIRGFFFADVGSAWYDKGAFSPELSADAFYDPAFGLIRADYS
                     GLTPAIIPWDFWDSENDRLQDGRASYGVGFQFLFLGGLQFNWTWAKRLPHTQYLYAVD
                     PFTGALDLSQPPVPVEEDNDTVQTEFYIAFDW"
     gene            4475..4966
                     /locus_tag="VD788_08285"
     CDS             4475..4966
                     /locus_tag="VD788_08285"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXV76303.1"
                     /translation="MRQDGFELAGRVSDFREGRGRVVKIAGTDVAVFKVGGRFFALRD
                     ACPHMGASLADGKLVGGCVECHWHHWRFDLRTGQGDQRSWAVAAVYQVRVERGELWLK
                     PPERVAPPAADEPPEGDGWVRWDPERFLRKRRAASDRSPDGEERADQGDGQPDDDQQS
                     GER"
     gene            complement(4883..5788)
                     /locus_tag="VD788_08290"
     CDS             complement(4883..5788)
                     /locus_tag="VD788_08290"
                     /inference="COORDINATES: protein motif:HMM:NF037183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HXV76304.1"
                     /translation="MKQATWTNCLRYFVADAWDEWRHSRGVNLLALGTLTATLFVAGL
                     MLLLLSNLERQLTVRRDHVQVQVYLYDETTPDARVRIERLVGTMPGVERVDYVDKTEA
                     LRRYREWAGQLAPALGELDGNPLPTSLEVTLVAGPAAEEQGEAIVAALVENPVVEEVR
                     FDRDWLRKLAALLDLARVGGGALALLVLAAVVFVMASVLRLAVYARRDEIDIMLLVGA
                     TPGFVRGPFLVAGLAQGLVAGALAVAAVETVRRAALAYAGRGSPALVDLVLERALPLP
                     FAGLLIVVGLAVALVSAFFAVRASI"
     gene            complement(5785..6456)
                     /gene="ftsE"
                     /locus_tag="VD788_08295"
     CDS             complement(5785..6456)
                     /gene="ftsE"
                     /locus_tag="VD788_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HXV76305.1"
                     /translation="MIRFYGVTKKYDGDVTALHDVGFEIERGEFAFITGSSGAGKTTL
                     LRLMLRQEEPTAGQIIVNGRNVAALPAGKVPDLRREIGVVFQDFKLIPRKTVLDNVAY
                     VLNVAGFGRNERRRRAYNALRSVGLSHRLSALPRVLSGGEQQRVAVARAVVNEPTLLL
                     ADEPTGNLDPDLAVEMMRMFREINARGTTVVVATHDRDLIQRMQRRTLVLHHGRLIDD
                     GSGER"
     gene            complement(6472..>8233)
                     /locus_tag="VD788_08300"
     CDS             complement(6472..>8233)
                     /locus_tag="VD788_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXV76306.1"
                     /translation="IGLPEVQIGIIPGFGGSQRLPRLIALPAAIDLILTGKRIDARRA
                     RRLGLVDAVVPPAYLEREAVALLERAIEVGVARAIGGARRRKRPVERLIESVPPLRRW
                     VLDHAHKKTEQRIRARDYPAPFRALEAIEAALTKPLPEGLDLEARIVGELVPTQTSKN
                     LIWLFKSQSALKSADVGLQATPRTVRRVAVLGAGVMGGGIAQAVAQQDLPVRLKDIRP
                     EPILAALRTAREIWDRQQRRGRIDEREIAQKMANISPTADDTGLVHADLVLEAVVENL
                     DVKRKVLAEAERRIDARAVFASNTSSLPISDIASRAIHPERVVGLHFFNPVHRMPLVE
                     VIAGRHSSPEAVATVRAFSIRLGKVPVLVRDEPGFLVNRILTFYLNEAMVLLSEGVRI
                     EAVDEAMTGFGMPVGPFALLDQVGLDTARHVEQVLAAAFGSRLGASASPLEVMVAAGR
                     LGQKSAKGFYRYRDAKRTTVDGEVYKLLGVKTAIDLPAETIQERLLLTMTNEAAVCLQ
                     ESVAREPRDVDLALVLGTGFPPFRGGLLRHADWVGIPIVADRLSRLADAHGERFRPTS
                     LLTRMVRDQRRFYPDQFAHG"
BASE COUNT         1372 a         2827 c         2701 g         1333 t
ORIGIN      
        1 aggaaacgca tcgacgtgcc ggactgccgt agcgcgatcg tcgcgccccc cgccaccgcg
       61 ccgccacgtc cctcgacgcg ccagcgatcg ccgtcgtctt cgatcgggat gccgagcgcg
      121 cgcaacccgt cgagcgtgac gcgcgtgtcg tcggcatcga gcggatcgag caggctgctc
      181 ggcccgacgg ccagtgcggc ggcgaccagc gcacggtgcg tcgcgctctt cgacggcgtc
      241 gcgcgcaaga ccgcgtcgag ctcgcgtctc gcgctgcgga tgtgcagcac gtccatctcg
      301 gcccagtgta gtatcatttt tccgagcggt ggtgggccgg tacgatcacc tgtggggggc
      361 acggctcgag gaggtccatg gcaacgtctc cgcgtcgaaa cgccttccga tggatcctcg
      421 ggtcatgctt cgtcgcgtcg tcgatcttgg tcgccggccc ggccggtcgg gccgacgaat
      481 cgggcggcaa ggaagcgatg aagttcgccg ccgacatggc cgcccgcggc aactggcgcg
      541 aggccagctt tcgttgggag aagctgctca cgtcgaccgg tggaaacgcc aagctctaca
      601 acaatctcgc cgtcgcatcc gaggccgtcg gtcggctcga gcgagccggc gagctgtact
      661 cgcaggcact cgacgaggcc accggcgatc cgctgatcgc ggacaactac cgtcgctact
      721 cccggctcgt ggcgctgttg ctcgagcggg atcccgatct gtccgacgaa ctcgacgcgc
      781 ccgaaatcga cgtcgacgat cagggggaca aggccaaggg caggtcggcg cgcgtcggtc
      841 tgaacgtgcc gatcccgccg cggatcgatc tcgacggaat ccagtcgatc ctggtgacct
      901 cgtttcgcgc caacgagtcg gggttcatcg acgtcaaccg cgagctggcc cgctttctcc
      961 gcggggagtt ccgccagcgc accgcgctcg aggtgctcga cgtcgtcccg ccaccggcga
     1021 tccccgagca gcggctcgag gacctggccg ccaacagcga gttctggaag tacctcgccc
     1081 gggaatacgg cgcggacctg atcgtctcgg ggatcgtcaa cttcggtcgc gtggaggttt
     1141 cgggattcga ggacgtcgac gtgatcaacc cgcggaccgg ccagaagatg cggcagtcgc
     1201 agttcgtcga gaaggaagag ttcaactacc aagtcgatct gttgttcatc gacgggtcga
     1261 ccggcagccc gttgcaccgt gatcggctca cccgcaaggt tctcttccgc gggttgagca
     1321 acgacccgtt gcatgccttc tacgaactga gcgaatcgct cggcgacgac atcgtctcgg
     1381 tcgtatcgac gcgtatgcgg gccgagtcgc gggtgatctt caagcgctag gcacccgccg
     1441 aggagaccaa gcatgcgcca gatcgacgtg cgaaagagcc ttcggtggtc gtggttgctc
     1501 gcggccgtcc tgctggcgtc gtcggctgac gcgcagtact tcggccagaa caagatcgcc
     1561 tacgcgaact tcgactggca ggtctacaag tccccgcatt tcgacgtcta ttactatcct
     1621 gagaccgagc cgttcctcga ggacatcgtc agctacgccg agagcgccta cgtccacctc
     1681 tcgcgcacgc tcgaccacga gctgcgattc cgcgtcccgc tgatcaccta caagacccac
     1741 ggcgagttcc agcagaccaa catcacgttg gccgagctgc cggacggggt cggcgcgttc
     1801 gccgagccgg tgcagtaccg catggtgctg ccgatcgatc tgccccccga cgagctgtac
     1861 gcgctgatcg cgcacgagct gacgcacatc ttcgagtact cctacttcta cgacggctac
     1921 ctcgggcggg cgattcgcgg tcgggcaccg acgtggctca tggaagggct ggcgtcctat
     1981 ctcggcgacg acgagacgaa cctcgaccgc atggcgattc gcgacgcggt cgtcaacaac
     2041 atcctcccgc cgatcgagtc gctcaacgtg gtcaccttcc tgacctaccg ctacggccac
     2101 gcgatcttcg actacatcga acaggagcac ggcatcgagg gcgtgcgcac cttcctgttc
     2161 gagttcaaga aggtgttgct gaccggcaac ctgtcgaagg cgatcgaaga atcgctgggc
     2221 tacgacatcg acgagttcaa tcgcagattc aaccgctacc tgcgccagaa gtactttccg
     2281 gtgctgctcg aaaagaagtc gcccgacgac tacggtaccg agctgggcac gcgcaagctg
     2341 cgcggcgtct tcacgttctc gccggcgctg tcgccctcgg gcgaactggt cgcggcgctc
     2401 ggcgtgcccg gcatggagct cgatctcgtc gtgctgtcgg ccgaagacgg cacgctggtc
     2461 aagaacctga cgaagggctg gaccaacaag taccgcaacc tggtggccga agtgttcaag
     2521 ggaaagcgcg acctgtcgtg gtcgccccgc gaggaccacg tcgcggtctt cgtccggcgc
     2581 gagaaccgct ggccattgtt cattttcgac gcgctgtcgg ggaacatcgt cgaccagatc
     2641 gcgttcgacg acatctacca gaacgcgagc ccggccttct cgaccgacgg acgtcgcatc
     2701 gcgttcgagg gcaaccgcaa cggcatcgtc gacattttcg aggtcgacct cgacacgcgc
     2761 gaggtgcgca acctgacgca ggacgacttc ttcgacgcca atccgtggta cggacccgac
     2821 gggaagacgt tgttgtacaa ccgccggatc ggcgagcact ggaaggtctt ctcggtcgac
     2881 ctcgaggatt cgtcgaagaa aacgcagctg acattcgggc cttacagcga gttgcagccg
     2941 tcgtactcgc gcgacgggaa cacgatctac tacgcgtcgg atcgcggcga gtacggcgtg
     3001 ttcaacctgt tttcgctcga cctccaaacc ggcgacctcc gccagtacac cgacgtcgtc
     3061 ggcggttgct tctcgccggt cgaggccggc caggtcggcg acgagacctt tctggtcttc
     3121 accgcctact accgcgggat gttccggctc taccgcatgc cgctgcgcgc tcccgaggcg
     3181 acgatcggca tggcccagcg gctggaggag ccgaccgaag ccgagccgtt cgagcccgac
     3241 atgcgactgc gcaccgacga ggatcagaaa gcacggtaca agctgaagtg ggacatcgaa
     3301 gcgccggacg tcggcgtcgg cgtggccaac gacggaacgt tcctcgccaa cgcggccatc
     3361 tcgttctccg atctgatggg cgaccaccgc gtgacgctgg cgatcgagag cgtgtcgagc
     3421 ttccagtcgt actgggcgca atacaccaac atcaagcgcc ggggcaactg gggtgcgcgc
     3481 gtctacgacg tgcgcgattt catcgtctcg caggtcgacg ggtcgcggct cgagcaggga
     3541 tcgcgcgcga cgggcgccga tctgttctac gagtacccga tcagccgtta ctatcgattg
     3601 gatttctccg tcggcgttgc cgacacgagc tacctgacgg tcgagggctt cgacgcggtg
     3661 agcggggaag cgatcttcgg caacatcgag gacacgctgg gcatcgtcag cgccgacctg
     3721 gtcggcgaca cgacgcgcta ccagcgctgg gggccgttcc agggcaagcg gttccgcatc
     3781 ggcgccgagt acgggccgcg actcggcggc gaccggtccg gcgacatgat tcgctacaac
     3841 gtcgattttc gcgcctacaa gcaggtgacc cggcgttcgg tgctcgcgtt ccgcaccgcc
     3901 ggcgtgatcg gtgcgggcga gatccagaac tcgtattcgt tcgggggcat caatcagcta
     3961 cggggatacg agtaccgcga gttcaccgga tcgcgcatcg cctggatgaa cctcgagttc
     4021 cgcttcccgt tggtcgacga gatgctcttc ccgatcctgg cgctgcagga catccgcggg
     4081 ttcttcttcg ccgacgtcgg ctcggcctgg tacgacaaag gcgcgttcag tcccgagctc
     4141 agcgccgacg cgttctacga cccggccttc ggcctgatcc gcgccgacta cagcggcctg
     4201 acgccggcga tcatcccgtg ggacttctgg gattcggaga acgaccggtt gcaggatggc
     4261 cgcgcctcct acggcgtcgg gttccagttc ctgttcctcg gtgggctgca gttcaactgg
     4321 acctgggcca agcggctgcc acacacccag tacctctacg ccgtcgaccc gttcacggga
     4381 gcgctcgacc tgtcgcagcc gccggttccg gtcgaggaag acaacgacac ggtgcagacc
     4441 gagttctaca tcgcgttcga ctggtgatcg gacgatgaga caggacggct tcgaactggc
     4501 cggtcgcgtc tccgactttc gcgaggggcg cgggcgggtg gtgaagatcg ccggcaccga
     4561 cgtggcggtg ttcaaggtcg gtggacgctt cttcgccttg cgcgacgcct gcccgcacat
     4621 gggggcgtcg ctggccgacg gcaagctcgt cggtggttgc gtcgagtgtc actggcacca
     4681 ctggcggttc gacctgcgta ccgggcaggg cgaccagcgc tcgtgggccg tcgccgcggt
     4741 ctaccaggtg cgggtcgaac gcggcgagct gtggctcaag ccgcccgagc gtgtcgcgcc
     4801 gccggccgcg gacgagccgc ctgagggcga cggctgggtc cgctgggatc ccgagcgctt
     4861 cttgcgcaag agacgggctg cctcagatcg aagcccggac ggcgaagaac gcgctgacca
     4921 gggcgacggc caacccgacg acgatcagca atccggcgaa cggtagcggc agcgctcgct
     4981 cgagcacgag atcgaccagc gccggcgatc cccgaccggc ataagccagc gcggcgcggc
     5041 gcaccgtctc gaccgcggcg acggccagcg ccccggcgac gagcccttgc gccaagcccg
     5101 ccaccaggaa cggtccgcgg acgaaacccg gcgttgcgcc gaccagcagc atgatgtcga
     5161 tctcgtcgcg ccgcgcgtag accgccaggc gcagcacgct ggccatcacg aacacgacgg
     5221 ccgccagcac cagcagcgcc agcgcaccgc cgccgacgcg tgccagatcg agcaacgccg
     5281 ccagcttgcg caaccaatcg cggtcgaatc gcacctcttc gaccaccggg ttttcgacga
     5341 gcgccgcgac gatcgcctcg ccctgctctt ccgccgccgg tcccgcgacc agcgtcacct
     5401 cgagcgaggt gggcagcgga ttgccgtcga gctcgccgag cgccggcgcc agctgaccgg
     5461 cccactcgcg atagcgccgc agcgcttcgg tcttgtcgac gtaatccacg cgttccacgc
     5521 cgggcatcgt gccgacgagg cgttcgatcc gcacgcgggc gtcgggcgtc gtctcgtcgt
     5581 acaggtagac ctggacctgg acgtgatcgc gccgcacggt cagctgtcgt tccaggttgg
     5641 acagcagcaa cagcatcaga cccgcgacga acagcgtcgc ggtcaacgtg ccgagcgcga
     5701 gcaggttgac cccgcggctg tgccgccact cgtcccacgc gtcggcgacg aaatagcgca
     5761 gacagttggt ccacgtcgcc tgcttcatcg ttcgcccgag ccatcgtcga tcaaccggcc
     5821 gtgatgcagc accagcgtgc ggcgctgcat ccgctggatc aggtcgcgat cgtgcgtggc
     5881 gaccaccacc gtcgtccccc gcgcgttgat ctcgcggaac atccgcatca tctcgaccgc
     5941 caggtcgggg tcgaggttgc cggtcggctc gtcggccagc aacagcgtcg gctcgttgac
     6001 caccgcgcgg gccacggcga cgcgttgctg ttcgccgccg gacaagacgc gcggtagcgc
     6061 ggaaagccga tgcgacaggc cgacgctgcg caacgcgttg taggcccgcc gccggcgctc
     6121 gttccggccg aagccggcga cgttgagcac gtaggcgacg ttgtcgagca cggtcttgcg
     6181 cgggatcagc ttgaagtcct ggaagacgac gccgatctcg cgtcgcaggt ccggcacttt
     6241 gcccgccggc agcgccgcca cgttccggcc gttgacgatg atctgcccgg cggtcggctc
     6301 ttcctggcgc agcatcaagc gcaacagcgt cgtcttcccg gctcccgacg aaccggtgat
     6361 gaaggcgaac tcgcctcgtt cgatctcgaa gccgacgtcg tggagcgcgg tgacgtcgcc
     6421 gtcgtacttc ttcgtcacgc cgtagaatcg gatcaccggt cgaacatccg ttcagccgtg
     6481 cgcgaactgg tcggggtaga atctgcgctg gtcacgcacc atccgggtca acagcgaggt
     6541 cggccggaaa cgttccccgt gcgcgtcggc caagcgcgac aggcggtcgg cgacgatcgg
     6601 gatgccgacc caatcggcgt gccgcaacag cccaccgcga aacggcggga agccggtgcc
     6661 cagcaccaac gccagatcga cgtcgcgcgg ctcgcgggca accgactcct gcaggcagac
     6721 ggcggcctcg ttcgtcatcg tcagcagcag acgctcctgg atcgtctcgg ccggcaggtc
     6781 gatcgcggtc ttgacgccga gcagcttgta gacctcaccg tcgacggtcg tccgtttcgc
     6841 gtcgcgatag cggtaaaagc ccttcgcgct cttctgaccg agacgcccgg cggcgaccat
     6901 cacttcgagc ggcgacgcgc tcgcgccgag gcgcgagccg aacgccgccg ccagcacctg
     6961 ctcgacgtgg cgcgcggtgt cgaggccgac ctggtcgagc agcgcgaacg gtccgaccgg
     7021 catgccgaat ccggtcatcg cctcgtcgac cgcctcgatt cggacacctt cggacaacaa
     7081 caccatcgcc tcgttcaggt agaacgtcag gatgcggttg acgaggaagc cgggttcgtc
     7141 gcgcaccagg acgggcacct tgcccagccg gatggagaac gcgcggaccg tggccacggc
     7201 ctcgggcgag ctgtggcggc cggcgatcac ctcgaccagc ggcatgcggt gcacggggtt
     7261 gaagaagtgc aagccgacga cccgttccgg gtggatcgcg cgcgaggcga tgtcggagat
     7321 cggcagagac gaggtgttcg aggcgaagac ggcgcgggcg tcgatacggc gctcggcctc
     7381 ggccagcact ttgcgcttga cgtcgagatt ctcgaccacc gcctcgagca ccagatcggc
     7441 gtgcaccagc cccgtatcgt ccgccgtcgg cgagatgttc gccatcttct gtgcgatctc
     7501 gcgctcgtcg atccgcccgc gtcgctgctg gcggtcccag atctcccgcg ccgtacgcaa
     7561 cgcggcgagg atcggctcgg ggcggatgtc cttgagtcgc accggcaagt cctgctgcgc
     7621 gaccgcctgg gcgatacccc cgcccatcac gcccgccccc agcaccgcga cgcggcggac
     7681 ggtgcgcggc gtcgcctgca acccgacgtc cgcgctcttg agcgcgcttt ggctcttgaa
     7741 caaccagatc aggttcttcg acgtttgcgt cggtaccagc tcgccgacga tgcgcgcctc
     7801 gaggtcgagc ccctccggca acggtttggt cagcgccgcc tcgatcgcct ccagcgcccg
     7861 gaacggagcc gggtagtcgc gagcgcggat ccgctgctcg gtcttcttgt gggcgtggtc
     7921 cagcacccag cgccgcagcg gtgggaccga ctcgatcaat cgctcgaccg gtctcttgcg
     7981 ccgcctcgca ccgccgatgg cgcgcgcgac gccgacctcg atcgcgcgct cgagcagcgc
     8041 caccgcctcg cgttcgaggt acgccggcgg cacgacggcg tcgaccagac cgaggcgacg
     8101 ggcgcgtcgc gcgtcgatcc gtttgccggt caagatcaga tcgatcgccg ccggcaaggc
     8161 gatcagccga ggcagccgct gcgatccacc gaagcccggg atgatcccga tctgcacctc
     8221 cggcagaccg atc
//
LOCUS       DASPGN010000284         7749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1690195, whole genome shotgun sequence.
ACCESSION   DASPGN010000284 DASPGN010000000
VERSION     DASPGN010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690195"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2131
                     /locus_tag="VD788_08305"
     CDS             <1..2131
                     /locus_tag="VD788_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HXV76307.1"
                     /translation="EVARSYDPENPWLQQPLTVNVNVASSCNANWSGSVINMFGAGGN
                     CDNTGENSGILVHEWGHGYDQNDGGGRDAPSEAYADIVAILTTRDSCMGRGVHNDGST
                     CNDFGEACLTCTGFRDFDWTARQANTPATPTDFAQVLCPTDTSIFAGPCRREAHCESY
                     VSSEAIFDLATRDLPAAGMDSGSAWQLVERLWFETRPGSGGNAYTCLLPLSNSCSATS
                     WYQRMRVADDDDGDLANGTPHAAAIFAAFARHAIACGVASDATNQSTSSCPALDTPEL
                     MRAMTPDGVELSWTAVAGAATYRVYRGELGCDRQQVALASIPAGQSSYVDSSADSGVL
                     RHYRVEAFGSNPACTSRVSNCVGAPPGSRLQPVSHRVEDDDGDGIPEPGETLSLPVTL
                     LNTGADAAYGAAADVVRVGTAEVRALVPTASWPTIEPNIPAESDAPHLELVVLAQARC
                     GDTLALDLSGAAANAAPFHAEIRIPMGEPQRDWVETAIVPVPSLTSTPVEVHFDVADD
                     RPIADLDVTLDIFHQDPTQLIVELTSPQGTTVRLHDRSTGSGHGIETRFDLDTAPDGP
                     GSLDDFHAESTLGSWTLSVQDVDASGVTTDGYIRSRTLHVTIDGAWDCEPRSCPDPVP
                     VEAPDLRVERIDGENTVDLNLSWSPVAGAGYHVLQSVDPTFGVGVELIGNLASETSLV
                     LPDGARTTPDLTFFQTRAVNACHHEGP"
     gene            2218..2958
                     /locus_tag="VD788_08310"
     CDS             2218..2958
                     /locus_tag="VD788_08310"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HXV76308.1"
                     /translation="MGFEPKQILISCEHASNRLPPELEAPTELLDLHVAWDPGALIVA
                     ERLAARFAAPLHQGRYSRLVVDLNRTVGNSRLIRRVSDGHRIPFNRGLRPGEIERRIE
                     RYYRPYRQAVERGVTEIVRREGRCVHLTIHSFTPVLRGKVRGNDVGLMYDPSRRPEAD
                     LMRELRGKLTEATGLVVWLNKPYSGTADGIQPRFREAYPADRYVGIELELNQKHAADT
                     RASIAIADAFAGALEGLRWLKAPAALSA"
     gene            3592..5025
                     /locus_tag="VD788_08315"
     CDS             3592..5025
                     /locus_tag="VD788_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76309.1"
                     /translation="MHSHKLEPEYLGPLADAPATDTGTHVLFPGEDRFLSGSACRKRL
                     ELVQALDATLERCLEDGERVVYVAPANQRPGLFESIGLGVWFYHLHRTALVFTDRRLV
                     EVLLEGTRNRPATRIRSWAWSGLSRVKFGFGALKLKPDAGRAASWRISLRPDRKVIKK
                     LLLNIRAKVVHVQGVENAHRWLCPTCGAANPPSPPSCGGCRTAFRTPGVATALSLALP
                     GGGLFYAGRPVLGTLDLLGELMLFGIVALSLAAAPGIAEGVAAAAVGLLLLVLTKVES
                     VHLSRMLVRRTVPETGDRRRTWKRIGVGGAIASSLAVIGAVALTGALAGSLVRDLEFG
                     EGERWTGTRAAGEFLAYDVDQRSQWTSGDGSAVFVAAHPLGIGEEWASFRESYVATIE
                     QDGLEPILLDDEVPTGFAGFRCYVPVEDDDGQPYYSLNYMLYDDESTAVHHVYTFVEP
                     ERFELRARELSDLVQTATWIDAVEPTL"
     gene            5050..5820
                     /locus_tag="VD788_08320"
     CDS             5050..5820
                     /locus_tag="VD788_08320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV76310.1"
                     /translation="MARKDFAPIEADYEFFRTHSTEAQHDAEDYARRLGSFADDREAI
                     AMLDFGCGAGAFTETFLERMSWPPERLTLSLIEPVTAQREHAAARLARFSAETVRHAA
                     ALTRGSKQRFDLVLSNHVLYYVEDLAGTLGELVGRLRPGGRLLLAMADRANALIDMWD
                     AGFGALGRAVPYHTAEDVVGVLESAGLHFATTESRYVIRFEDRPSHRLSILRFLFGEH
                     LDRMPREKLLAGFDRYVSRGAIEIATHDLHFDLTLDGS"
     gene            complement(5817..6224)
                     /locus_tag="VD788_08325"
     CDS             complement(5817..6224)
                     /locus_tag="VD788_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV76311.1"
                     /translation="MIIFFMVTSVFSATKGLELTLPEDDESVDRTPEPAVVVHVEAEH
                     LYIDCQETTIDGILPYLEPILTRNPNKPVVLYTDPDAEYRRMVAVYDVLAAAGGEHSQ
                     YSFRVRDISVPTRSDVLAYVELFGENPFQTACR"
     gene            6208..6690
                     /locus_tag="VD788_08330"
     CDS             6208..6690
                     /locus_tag="VD788_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76312.1"
                     /translation="MKKMIKRKAMSAIDEVGMDESAEDRRSSFIESLHGSGVIDGTIV
                     GRARAGDRRRDESVRGSNRNAKLGTQPRPSGRWAGEVAGAHYPAAESPAFLGGAPGEL
                     GCVSAARGRGTAGRTSLPIFMTDFQKLITALAEGGVEVVVDVAYSRSPENTSVIPAVA
                     "
     gene            complement(7148..>7749)
                     /locus_tag="VD788_08335"
     CDS             complement(7148..>7749)
                     /locus_tag="VD788_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76313.1"
                     /translation="LAASRRPGIAFLILDKGAAVTVEEQELQRVEYGLRAAKLTESQI
                     AEALAYTKLVFHVAYGGEGWPELVAATNEARARKWAETVQLVETENDLEGWRQQRFDP
                     APILRRTKIPVLALFGGNDTLVPPAGNAERMRGYFEEAGNGDVTIRIFPAQGHSRFLG
                     QSLKGDRWEWPDRYWIWDKQAPEFEETVISWLTMRLGLE"
BASE COUNT         1210 a         2682 c         2634 g         1223 t
ORIGIN      
        1 agaggtcgcc cgctcttacg acccggagaa cccctggctg cagcagccgt tgacggtcaa
       61 cgtcaacgtc gcctcgagct gcaacgcgaa ctggagcgga tccgtcatca acatgttcgg
      121 tgccggtggc aactgtgaca acacgggcga gaacagcggg atcctggtcc acgagtgggg
      181 ccacgggtac gaccagaacg acggcggagg gcgggacgcg ccgtccgagg cgtatgccga
      241 catcgtggcc atcctgacca cgcgcgactc gtgcatgggt cggggcgtgc acaacgacgg
      301 cagcacgtgc aacgatttcg gtgaggcttg tctgacctgc accggctttc gcgacttcga
      361 ctggaccgcg cggcaggcga acaccccggc cacgccgacc gacttcgccc aggtgctgtg
      421 tcccacggat acgtcgatct tcgccggacc gtgccgccgc gaggcccact gcgagtcgta
      481 cgtgtcgagc gaggcgatct tcgatctcgc cacgcgcgat ctccccgcgg ccgggatgga
      541 ctccggcagc gcctggcagc tcgtcgagcg actgtggttc gaaacccgtc cggggtcggg
      601 aggcaacgcc tacacgtgcc tgctgcctct gtccaactcg tgcagtgcga cgagctggta
      661 ccagcgcatg cgcgtggcgg acgacgacga cggcgacctg gccaacggca cgccgcacgc
      721 cgcggcgatc ttcgccgcct tcgcacgaca cgccatcgcc tgcggcgtgg cctcggacgc
      781 gacgaaccag agcacgtcga gctgccccgc gctggacacg ccggagctga tgcgggcgat
      841 gactcccgat ggcgtcgagc tgagctggac cgccgtcgcg ggcgcggcca cctaccgcgt
      901 ctaccgcggc gagctcggct gcgatcgcca gcaggtcgcg ctggcgtcga tcccggccgg
      961 gcagtcgagc tacgtcgaca gcagcgcgga ctccggggtg ctcaggcact accgggtcga
     1021 ggccttcggc tcgaaccccg cgtgcacgag ccgggtgtcg aactgcgtcg gcgcgccacc
     1081 gggaagccgc ctccagcccg tctcgcaccg cgtcgaagac gacgacggcg acggcatccc
     1141 cgaaccgggc gagacgctga gcctcccggt cacgctgctc aacaccggag ccgacgccgc
     1201 gtacggtgcc gcggccgacg tggtgcgggt cggcacggcg gaggtgcgcg ctctcgtacc
     1261 gaccgcgagc tggcccacga tcgagccgaa catcccggcc gagagcgacg cgccgcacct
     1321 cgagctcgtc gttctggcgc aggcacgctg cggggacacg ctggcgctcg acctgagcgg
     1381 cgcggccgcc aacgcggccc cgttccacgc cgagatccgg atcccgatgg gcgaaccgca
     1441 gcgagactgg gtcgagaccg cgatcgtccc cgttccttcc ctgacctcca cgcccgtcga
     1501 ggtacacttc gacgtcgccg acgatcggcc gatcgcggat ctcgacgtca cgctcgacat
     1561 cttccaccaa gatccgacac agctgatcgt cgagctgacc tcgccacagg gcaccacggt
     1621 tcgcctgcac gatcgcagca ccggaagcgg ccacggcatc gagacccggt tcgatctcga
     1681 caccgcgccc gacggcccgg gcagcctcga cgacttccat gcagaatcca cgctcggcag
     1741 ctggaccctg tcggtccagg acgtggacgc atccggggtg acgaccgacg gctacatccg
     1801 ctcgcgaacc ttgcacgtca cgatcgacgg cgcgtgggac tgcgagccgc ggagctgccc
     1861 cgacccggtt ccggtcgagg ctcccgacct ccgtgtcgag cgaatcgacg gcgagaacac
     1921 cgtcgacttg aacctgtcct ggagccccgt cgccggggcg ggctaccacg tcctgcagtc
     1981 ggtcgatccc acgttcggcg tcggcgtgga gctgatcggg aatctcgcaa gcgagacgag
     2041 cctggtgctg ccggacggcg cccggaccac gcccgacctg accttcttcc agacccgggc
     2101 ggtcaacgcc tgccaccacg aaggcccctg agccggacgg gcgcccgcct gggctatact
     2161 cgcggcttcg accagcgcgg ccgcgcagga gcgaagtcgt ctacccgacg gggcgtcgtg
     2221 ggtttcgagc cgaagcagat cctgatcagc tgcgagcacg cgtcgaaccg cctgccgccc
     2281 gagctcgagg ctcccaccga gttgctcgac ctgcacgtcg cttgggaccc cggggcgctg
     2341 atcgtcgccg agcgcttggc cgctcgcttc gcggcgccgc tccaccaggg acggtactcg
     2401 cgcctggtcg tcgacctgaa ccgtaccgtc ggcaactcgc ggttgatccg gcgggtgtcc
     2461 gacgggcacc gcatcccgtt caaccgcggc ctgaggcctg gcgaaatcga gcgccgcatc
     2521 gagcgctact acaggccgta ccggcaggcg gtcgagcgcg gcgtgaccga gatcgtccgg
     2581 cgcgaggggc gctgcgtgca cctgacgatc cattccttca ccccggtgct ccgaggcaag
     2641 gtgcgcggca acgacgtcgg gctgatgtac gacccgtcgc gccgtccgga ggcggacctg
     2701 atgcgcgagc tgcgggggaa gctgaccgaa gccaccggtc tcgtcgtctg gctgaacaag
     2761 ccgtacagcg gtacggcgga cggcatccag ccccgttttc gcgaggcgta cccggcggac
     2821 cgctacgtgg gcatcgagct cgagctcaac cagaagcacg ccgccgacac gcgggcatcg
     2881 atcgcgatcg ccgacgcttt cgccggcgcg ctcgaggggt tgcgttggct caaggctccg
     2941 gcagcgctct cggcgtgagc gcccgccggg cgtcgtcgcg catccgccgg tcgagcggcg
     3001 acccggaggc cgagaggtcg cccgcgggcg tgcggacctc gccgagcgac cgcaggttct
     3061 gccggccgag cggatgctcc gccccggccg ccgcgaacac ctcgcggcga cgttgaagaa
     3121 gcgctgcgtc gcgtgggcgt ggacgacgcc cggcatcagc gacaggtagt agtcgagcgt
     3181 tgccggcggg atcgtctgcg agctggcgta gaccacgcgc gcggcgggct ggcacagcaa
     3241 cagcagcatg aacagcatgc gctcctcgta ggcctgcatt tcggcgccct ggcagtcgaa
     3301 gtcgagcgac tgcgacggca ccacgacgat ggtctgcgcg ctgtcgttga tgcgacggat
     3361 ccgctgccat agcgggacga gcttgcgctg cagtcgctcg aatcgtcgct gcgcctcgtc
     3421 ctccggtagg ccgagcggcg ggatgtcgag cggtggggtc atcaccatga tccggcctcc
     3481 cgatcggctg gggtcgcggg ccgattctac gagagaaccc gacggcaact ggccgagacc
     3541 gcctcgcggc ctatattcgt agggtcgaac ccggggggca tggagcggtc catgcactca
     3601 cacaagctcg agcccgaata cctcggacct ctggccgacg cgccggcgac ggacaccgga
     3661 acccacgtgc tgtttccggg cgaagatcga ttcctgtccg ggtccgcctg ccgcaagcga
     3721 ctggaactgg tccaggccct ggacgcgacc ctcgagcgct gcctcgagga tggcgagcgc
     3781 gtcgtttacg tcgcgccggc caatcagcgg cccgggctgt tcgagtcgat cgggctcggc
     3841 gtgtggttct accacctgca ccgcaccgcg ctcgtcttca ccgaccgccg cctggtcgag
     3901 gtgctcctgg aaggcacccg gaaccggccg gcgacgcgta tccgctcgtg ggcctggtcg
     3961 gggctgtcgc gggtcaaatt cggcttcggt gctctcaagc tgaagcccga cgccggtcgc
     4021 gccgcatcgt ggaggatctc gctccgccca gaccggaagg tcatcaagaa gctgctcctc
     4081 aacatccggg ccaaggtcgt gcacgtccag ggggtggaga acgcgcatcg ctggctctgc
     4141 ccgacgtgcg gcgcggcgaa cccgccctcg cccccgtcct gtggagggtg tcgcacggcg
     4201 ttccgcaccc cgggcgtggc gaccgcgctg tcgctcgcgc ttcccggcgg tgggctgttc
     4261 tacgccggtc gcccggtcct gggaacgctc gacctgctcg gtgagctgat gctgttcggc
     4321 atcgtcgctt tgtcgctcgc ggccgccccc ggtatcgcgg agggagtcgc ggcggcggcg
     4381 gtgggcctgc tgctgctcgt gctgacgaag gtcgagagcg ttcacctgag ccgcatgctc
     4441 gtgcggcgga ccgtccccga gaccggcgat cggcgccgca cctggaagag gatcggagtc
     4501 ggcggggcga tcgcctcgtc tctcgccgtg atcggagcgg tcgcgctgac cggggcgctc
     4561 gcgggctcgc tggttcgcga tctcgagttc ggcgaaggtg aacgctggac cggcacgcgc
     4621 gcggccggcg agttcctcgc ctacgacgtc gatcagcgat cgcagtggac gtcgggcgac
     4681 ggttccgccg tgttcgtcgc ggcccatcca ctcggcatcg gggaggagtg ggcgagcttc
     4741 cgcgagagct acgtcgccac gatcgagcag gatggcctcg agccgatcct gctcgacgac
     4801 gaggtcccca cgggcttcgc cgggttccgc tgctacgtgc ccgtcgagga cgacgacggc
     4861 cagccgtact actcgctgaa ctacatgctg tacgacgacg aaagcaccgc cgtgcaccac
     4921 gtctacacgt tcgtcgagcc cgagcggttc gagctccgcg cccgagagct gtcggacctc
     4981 gtgcagaccg cgacgtggat cgatgcggtg gaaccgacgc tctgatccga tctgttattc
     5041 tgtcgtcgga tggctcgaaa ggacttcgcg ccgatcgagg cggactacga gttcttccgc
     5101 acgcactcga ccgaggcgca acacgacgcc gaggattacg cccgacggct cgggtcgttc
     5161 gccgacgacc gcgaggcgat cgcgatgctc gatttcggct gcggcgcggg tgcgttcacc
     5221 gagacgttcc tcgagcgcat gtcatggccg ccggagcggc tgacgctgtc gctgatcgag
     5281 ccggtgaccg cgcagcgcga gcacgccgcg gcgcggctcg ctcggttcag cgcggaaacc
     5341 gttcggcacg ccgccgcact gacacgcggt tcgaagcaac gcttcgatct cgtcctgtcg
     5401 aaccatgtgc tctactacgt cgaggatctc gcgggaacgc tcggtgagct ggtgggcagg
     5461 ctgcgccccg gcggacggct gttgctcgcg atggccgacc gggccaacgc gctcatcgat
     5521 atgtgggacg ccggcttcgg cgcgctcggt cgcgcggtgc cgtaccacac ggccgaggac
     5581 gtcgtcggcg tgctcgaatc cgccgggctc cacttcgcta ccaccgagtc gcgctacgtc
     5641 attcgcttcg aggatcgccc gtcccaccgg ctgagcatcc tgcgcttcct gttcggcgag
     5701 cacctcgacc ggatgccgcg cgagaaactg ctcgccgggt tcgatcgcta cgtcagccgg
     5761 ggtgcgatcg agatcgcgac ccacgacctg cacttcgatc tcacgctaga cggaagctag
     5821 cgacaggccg tctggaacgg attctccccg aacagctcga cgtaggcgag gacgtcgctg
     5881 cgcgtcggca ccgagatgtc acgcacgcga aacgagtact ggctgtgctc gccccccgcg
     5941 gcggcaagca cgtcgtagac ggccaccatc cggcggtact cggcatcggg gtcggtatac
     6001 agcacgaccg gcttgttcgg gttgcgtgtc aggatcggct cgaggtacgg caggatcccg
     6061 tcgatcgtcg tctcctgaca gtcgatgtag aggtgctcgg cctcgacgtg gaccaccacc
     6121 gccggttcgg gcgtccgatc gaccgactcg tcgtcttccg gcagcgtcag ctccagtccc
     6181 ttggtcgccg agaacacgct cgtcaccatg aagaagatga tcaagaggaa agcgatgtcg
     6241 gccatcgacg aggtggggat ggatgagtcg gcagaagacc ggcgttcgag tttcatcgag
     6301 tcgctccacg gatcgggcgt gatcgacggg acaatcgtcg gtcgagctcg agcgggtgat
     6361 cgacggagag acgaatcggt gcggggatcg aaccgcaacg cgaagctcgg cactcagcct
     6421 cggccgtcag gacgatgggc tggtgaggtc gccggcgctc actaccctgc ggcagaatct
     6481 ccggcgttcc ttggcggagc gcctggcgaa cttggttgcg tttcagcggc tcgcggaaga
     6541 ggcacggcgg gccggacatc gctcccgatc ttcatgacgg acttccagaa gctgatcacc
     6601 gccttggccg aaggcggtgt cgaggtcgtc gtggatgtcg cctactcccg ttcgccggag
     6661 aacacttcgg tcattcctgc cgttgcctga acctgccgag cttgatccgc atcaagcgtg
     6721 ccgcgggtcg accgactcga ggcccttctc gaggagcgcg agaaccgcta gcggacgacc
     6781 cgctggacat gatgacggag acgcgaccgg ctgcgtcgtc ctccgctatc tgggtgaaag
     6841 gccagcatga tcgatccggc agagcggcag ccggtttgcc tggatccggg ttcagtcctc
     6901 gtcgatgtcg taggcgaacc gcgtgaccag ccgcgaacgt acgatcgcct ggtcgggtgc
     6961 cgtcagttcg aagcgctcca catccgcgcc caccgggagt gtcatcccat tcggcaggac
     7021 gtcgaaacgg aggtgtaccc gttcggcaat cggtcgcctc ttgaaacgaa cgagctgcac
     7081 gaagaagatg agcttgaacg accggacgtg ctcgcgtcgc tgtcgttcaa gacgaggcca
     7141 cagcccgcta ctcgagtccc aggcgcatgg tgagccacga gatcaccgtc tcctcgaact
     7201 cgggcgcctg cttgtcccag atccagtatc gatcgggcca ctcccagcgg tcgcccttca
     7261 gcgactgccc gaggaaccgg ctgtggccct gtgcggggaa gatccggatc gtgacgtcgc
     7321 cgttgcccgc ctcttcgaaa tagccgcgca tccgctcggc gttcccggcg ggcggcacga
     7381 gcgtgtcgtt gccgccgaac agggccagca ccgggatctt cgtccgcctc aggatcggag
     7441 cggggtcgaa acgctgctgc cgccatcctt cgagatcgtt ttcggtctcc acgagctgga
     7501 ccgtctcggc ccacttcctg gcgcgcgcct cgttcgtcgc cgccacgagc tcgggccatc
     7561 cttcgccgcc gtaggcaaca tggaagacga gcttcgtgta cgccagcgcc tccgcgatct
     7621 gcgactcggt cagtttggct gcgcgcaggc cgtattccac gcgctgcagc tcttgctcct
     7681 cgaccgtgac ggcggctccc ttgtcgagga tgaggaacgc gatccccgga cggcgcgagg
     7741 ccgcgagcg
//
LOCUS       DASPGN010000285         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1690226, whole genome shotgun sequence.
ACCESSION   DASPGN010000285 DASPGN010000000
VERSION     DASPGN010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690226"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VD788_08340"
     CDS             complement(<1..219)
                     /locus_tag="VD788_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76314.1"
                     /translation="MVRSSLGIATAVGALVVLLLLVSCGGGERPATATPAEDAPQRIL
                     IIVTSGLAGDDATAIVAGFAADRPETVVF"
     gene            complement(296..1198)
                     /locus_tag="VD788_08345"
     CDS             complement(296..1198)
                     /locus_tag="VD788_08345"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HXV76315.1"
                     /translation="MLRLFTDILLPVIVAAGTGYWLAARFRLDVRSLSRVAFHLLAPC
                     LVFQAILDSELPGTEMLRMAGLAISVLLVSAAIAGGVARGCGWHREQVAAVVLVVLLP
                     NAGNFGLPTTLFAFGEEGLAQASIFFIAASLATFTIGVLVASSGRARPLAAARGLLRV
                     PAVWAIALALSVSRSGWTLPHALGRPIELFSDASIPVFLLILGMQLRGNGFEARFGPV
                     GWAVASRLLISPVVALVAAPLWGIDGVARQAAVLESAMPCAVISTVLAAEYDLAPRFV
                     TTAVFASTLISPVTLTPLLDYLGA"
     gene            1495..2118
                     /locus_tag="VD788_08350"
     CDS             1495..2118
                     /locus_tag="VD788_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76316.1"
                     /translation="MKRNLSWIAACFAVSVLALIGSAPVHAQATGQTAVSVDIPDIVI
                     LHYFSSVDVTIDANTMGSFLTGTPGNAGTVNEGVTSGLASLSGSNLQVDLAMTPSALT
                     GDPSAAVLVLQNAWAVRAISLGNLGSSTELDIAITGATMSNGGSQIGITTATVNDGTS
                     NASTITFPAPGLAPALQGDVELTLSFANAVDAGPHTGGEFTLTATNL"
     gene            2151..2759
                     /locus_tag="VD788_08355"
     CDS             2151..2759
                     /locus_tag="VD788_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76317.1"
                     /translation="MNFPTTILAGCEGRGPARQRVGPRLVSCLIVLAVAAVNAQDRGG
                     VGRDAIFASPIEPRLGVLEVRRFVDIERVAPAEFVGGSRSSGHYRPGGFATVDLELDT
                     RVLRSVPAVWRMRVGSVDALSEMNVRYELSTADGRTNLLAHAADPGSIVQARVDGLPP
                     TVVDVDDRGVLVEGGVMLHLDLQGVRAAGAHTATMTVTIEHF"
BASE COUNT          487 a          994 c          967 g          406 t
ORIGIN      
        1 gaagacgacg gtctcgggcc ggtccgcggc gaagcccgcg acgatcgccg tggcgtcgtc
       61 accggcgagc ccgctcgtga cgatgatcaa gatgcgctgc ggcgcgtcct cggccggtgt
      121 cgcggtcgcc gggcgctctc ccccgccgca cgagacgagc aggagcagga ccacgagcgc
      181 cccgaccgcg gtcgcgatcc cgaggctcga gcgcaccatc cccgcttcct ccatcggcta
      241 ccccgcaagg ttaccagatc cggcacgtcg gccacacgcc ggccgtcgca cgatctcacg
      301 ctcccaggta atcgagcagc ggcgtcagcg tcaccggact gatcaacgta ctggcgaaga
      361 ccgcggtcgt gacgaagcgg ggcgcgaggt cgtactccgc cgccagcacg gtcgagatca
      421 ccgcgcacgg catcgccgac tcgagcaccg cggcctgacg ggcgacgccg tcgatgcccc
      481 acagcggagc cgcgaccaac gcgaccaccg ggctgatcag caagcgcgac gcgacggccc
      541 agccgaccgg cccgaaccgc gcctcgaagc cgtttccgcg cagctgcatg cccaggatca
      601 acagaaagac cgggatcgaa gcgtcggaga acaactcgat cggacggccc agggcgtgcg
      661 ggagcgtcca acccgatcgc gagaccgaga gcgcgagcgc gatcgcccag accgccggta
      721 cgcgcaacag tccccgggcc gcggcgagcg gccgcgcgcg tcccgacgag gcgaccagca
      781 cgccgatcgt gaaggtcgcg agcgaggcgg cgatgaagaa gatgctggcc tgcgccaagc
      841 cctcctcgcc gaacgcgaac agggtcgtcg gcaatccgaa gttgccggca ttgggcagca
      901 gaacgacgag gacgacggcg gcgacctgct cgcggtgcca accgcaccca cgagccacgc
      961 cgcccgcgat cgccgccgag acgagcagga ccgagatcgc gagaccggcc atccgcagca
     1021 tctccgtgcc gggaagctcg ctgtcgagga tcgcttggaa gaccagacac ggcgcgagga
     1081 gatggaacgc cacccgcgac agcgatcgca cgtcgagccg gaagcgggcc gcgagccaat
     1141 agccggtccc ggctgcgacg atgaccggca acaggatgtc ggtgaacagg cggagcatgg
     1201 gacggcggag gataggtcag gtgaccgatt tcccgccact cgatgtcgcc cggccaccgc
     1261 gaactcgaca ttatttgtca cccgtcgcga tgctccccgt tcgtgcgtgt cgaggcaaca
     1321 ctcgaacggc cggcggttga aggtggagaa tctggcgcgt tcctcggcct cgccgcgagg
     1381 gacgttacga tcctgccggc tcggttccgc ccgtggcact tccattgcta ttccgaacgg
     1441 actcgaggcc cgtgctcgac cttggaatcc accacggaaa ggagaaccag tccgatgaaa
     1501 cggaacctgt cctggatcgc cgcctgcttc gccgtctccg tcctcgcgtt gatcggctcc
     1561 gcgcccgtcc acgcgcaggc gaccggccag accgccgtgt cggtcgacat ccccgatatc
     1621 gtcatcctgc actacttcag ctccgtcgac gtgacgatcg acgccaacac gatgggtagt
     1681 ttcctgaccg gaacccccgg gaacgcggga accgtcaacg agggtgtcac gtcgggtctc
     1741 gccagtctgt cggggagcaa cttgcaggtc gacctggcga tgactccgtc cgctctgacg
     1801 ggcgatccgt cggccgcggt gctggtgttg caaaacgcgt gggccgtccg tgcgatctcg
     1861 ctcggcaacc tcggctcctc gaccgagctc gacatcgcga tcaccggagc gacgatgagc
     1921 aacggcggct cccagatcgg tatcaccacc gcgacggtca acgacggaac gagcaacgca
     1981 tcgacgatca ccttcccggc tcccggattg gcccccgcac tgcagggcga cgtcgagctc
     2041 acgctcagct tcgcaaacgc ggtcgacgcc ggcccgcaca ccggcggcga gttcaccctg
     2101 accgcgacca atctctgatt cggcgctttc gccgggaagt acggggcgag atgaacttcc
     2161 cgacaacgat tctggcaggc tgcgaagggc ggggcccggc gcgtcagcgc gtcgggcccc
     2221 gtctcgtgtc gtgcctgatc gtgctcgcgg tcgcggcggt gaacgcgcag gatcgcggcg
     2281 gtgtcgggcg ggatgcgatc ttcgcctcgc cgatcgagcc ccggctcggt gtgctcgagg
     2341 tccggcggtt cgtcgacatc gagcgagtcg ctccggccga gttcgtcggc ggctcgcgct
     2401 cgagcggcca ctatcgcccc ggcggcttcg ccacggtcga tctcgagctc gacacccgcg
     2461 tcctgcgctc ggtgccggcc gtctggcgga tgcgggtcgg cagcgtcgat gcgctgtccg
     2521 agatgaacgt gcgctacgag ctgagcacgg ccgacggccg caccaacctg ctggcgcacg
     2581 cggccgatcc gggctcgatc gtccaggccc gcgtcgacgg attgccgccg accgtggtcg
     2641 acgtggacga ccgcggggtg ctggtcgagg gcggggtgat gctgcatctc gacctacagg
     2701 gtgtgcgcgc ggccggcgcg cacacggcga cgatgacggt cacgatcgag cacttctagc
     2761 gaggacggtt gggtaggacg agatgcagaa gatcgacgtt gcacacaagc tggttcggtt
     2821 gttcgtcttg gtggtcatcc tggttctcgg agtc
//
LOCUS       DASPGN010000286         8476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_519376, whole genome shotgun sequence.
ACCESSION   DASPGN010000286 DASPGN010000000
VERSION     DASPGN010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519376"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1414)
                     /locus_tag="VD788_08360"
     CDS             complement(<1..1414)
                     /locus_tag="VD788_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HXV76318.1"
                     /translation="MSGPIVRHDLVEHVREIENVFIPLHDGCRIAARIWMPDDVERNP
                     VPAILEYIPYRKRDFTRERDEPIHRYFAGHGYAAVRVDIRGSGDSEGVLTDEYTRAEH
                     ADAVEIIDWLARQPWCDGSVGMMGISWGGFNALQVAALRPPALRAVITLCSTDDRYAD
                     DAHYMGGCLLNENVQWGAILMTYGALPPDPQIVGERWRTMWLDRLDGAAPFAERWMRH
                     PWRDAYWKHGSVCQDYDAIECPVYAIGGWADGYSNAVPRLLAGLRGPCKGLIGPWAHT
                     FPHTGYPGPPIGFLQEAVRWWDEWLRGRTTGIMEEPRFRVWMQESVPPAPQYEERPGR
                     WVAEASWPSPRIELQRFRLNPGRLQHAPSQPTTIAFSSPQTTGLAAGEWCAFGADGEM
                     PLDQRPDDGRSLVFDGDALAERVEILGAPVVELDLSSDAPVAMIAVRLNDVAPDGTSA
                     RVSYGLLNLTHRDDHERPTPL"
     gene            complement(1424..2389)
                     /locus_tag="VD788_08365"
     CDS             complement(1424..2389)
                     /locus_tag="VD788_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76319.1"
                     /translation="MSRSMPLIERDPWRMQYFVGLDCPDDVVIPTEEQDAWKLFPRYR
                     WTFNKLLIADSQGLSCGLHGIDPPSYPVFSKPVYNMRGMGVGSRILRSPKEYERYQRP
                     GHIWMELLEGEHVSTDVAVVRGEARWWRHVLAKALGDGKFDYWTILAEARPEIERYCG
                     EWIARNLPDYTGIVNLETIGARIIEAHLRFADQWPDLYGRGWLDALVRLYARGVWDHA
                     DDDRRDGYSVVLFGAHGVQYRHPDRLLVERLLDHPRISSIQITFHEDKPTWAHSMPPG
                     GFRLAVINGSDLDAGIAARQELALAFWSTQRLVSRRGRVGVKLGG"
     gene            complement(2386..3177)
                     /locus_tag="VD788_08370"
     CDS             complement(2386..3177)
                     /locus_tag="VD788_08370"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HXV76320.1"
                     /translation="MRRFAVRWMAAGLVATAAHAGVVVEMEVRTPDSPDAVGSERFYA
                     EGEMVRMDPHRARGGGEMSAIFRDQTLWFVDHDKKQCQKIDREGVEQLSARLDQMMKQ
                     FEQMPPEQRAMMEKMMQGKMPGVSAPPSRRIDSGGTEQIGEYTCEVHTLYSDEKKVWE
                     VCAADESVAADVIEAMDAFRAMSRFTESLQRVVRDSAFVGMIDTPYTEMDDLGFPVRV
                     RTFDDKERLVSETTLASINRREIDAAVFEVPPGYKVKDLKDQLGK"
     gene            3751..4677
                     /locus_tag="VD788_08375"
     CDS             3751..4677
                     /locus_tag="VD788_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXV76321.1"
                     /translation="MSAPSHPPADLREQRHAMRLSLVVGLSMLLAKWYAFSITNSAAI
                     LSDAAESVVHVAAVAFAAFSLWLSLRPADSSHLYGHDKISFFSAGMEGLLIVLAAVYI
                     VYEAVARWIVGLELHNLGTGTIVVGAAAAVNLGLGIYLVRQGQRHRSLILVANGKHVL
                     TDSWTSLGVIGGLLLVLWSGWRPFDPLLAILVAINILWSGGKLVRESIGGLMDESDEE
                     THRVLVRLLDRETTGRGLSYHELRHRKSGAGTWVELHLLFPRETTIEAAHHHATKIEA
                     AIEAELPGPVTVTSHLEPRERHDEIHDRSRRR"
     gene            complement(4679..5368)
                     /locus_tag="VD788_08380"
     CDS             complement(4679..5368)
                     /locus_tag="VD788_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HXV76322.1"
                     /translation="MWHGELLDTALKVGSAWAAGSLIGLDRSFHSRPAGFRTHALVCI
                     ASALLMLLTGYRWLDDAPAEMLRIDPTRMAQGIMTGIGFLGGGVIFREGLTVRGLTTA
                     ASVWTTAALGILFGIGMYGPAVLGTALTIGILAVFRWVEGRIPWRYYAHYAVRFRNTR
                     VPSEVTVRGELHELGFVAGNMSYALSDDGTVFEYRTVINTGHKANASRLAERLRESAD
                     VLDFTIVPTGD"
     gene            5443..5739
                     /locus_tag="VD788_08385"
     CDS             5443..5739
                     /locus_tag="VD788_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76323.1"
                     /translation="MMKTIQNKTHKPLRIPLDGGHVLHLGPNKTGRVSDHAAERPAIR
                     RLVESGEIAILDDGGDTGDAGAETAGGPEVPAAAPQRKVVIPTGGRTAGGRRER"
     gene            complement(5768..6901)
                     /locus_tag="VD788_08390"
     CDS             complement(5768..6901)
                     /locus_tag="VD788_08390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV76324.1"
                     /translation="MHRRRQEPDGLPKTAATVVDGGAAADSLAVYLREAGSVPLLLPE
                     REFDLARRLALARRALRALARGAGRTLGRDVASTLGSRPRRGEPWTLEQVDRFVEDLV
                     RRCRARRDTGAAKLLAETRRHKRRLDDARDQLIRANLRLVVHIAKKYPHPNLALSDLV
                     QEGNLGLIKAVDKYDHRRGNRFSTYAYWWIKQAIERAIGNQARAVRVPVHVQDKARRI
                     RRASETLRARRRREPTTEEIAGELGLTTSKVHEILGSMRDNDPLEDPERTFDLVRTAS
                     DPQAVCPFEQVLSAQRRRVVEAALERLTPQEQRLIRLRFGLDGERRPTLQSVGEAMGL
                     SRERVRQIEHRALIKIRSNPTSAELGDLVDSDLPAAVDTRGPT"
     gene            complement(7008..7523)
                     /locus_tag="VD788_08395"
     CDS             complement(7008..7523)
                     /locus_tag="VD788_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HXV76325.1"
                     /translation="MSNSAEASIVTVTAACRAVELPGRGRGIVARRPIAVGEFVLAFR
                     GSELRFQEVTEFTHVLEYAPGRFLGPSGELDDLVNHSCDPNCAVETNGRAALLRAVRP
                     IRRGEELTFDYSTAMLTDPTTFPCQCGANGCRGIVRRWSELPSSVRGSYLRRGMVPAF
                     VARPPARPRRR"
     gene            complement(7513..7767)
                     /locus_tag="VD788_08400"
     CDS             complement(7513..7767)
                     /locus_tag="VD788_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76326.1"
                     /translation="MSETNVRVRRWRTKDIPALVECLGAAYREEFEQIVKCFGDTANE
                     YPCRFLVLPDEIGTVRPLVECRTDLYDVRAAVKLEVVHVE"
     gene            7867..8424
                     /locus_tag="VD788_08405"
     CDS             7867..8424
                     /locus_tag="VD788_08405"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXV76327.1"
                     /translation="MLRLTIGSLLVVLSAVAVAAEAPALSWTDLSGRRLALDDLRGRV
                     VVLNFWATWCEPCRRELPELVRVQTRYGVLGLQVLGAGADPPAQSEAVLRFARSRKLN
                     FPLVLGADAKQMEALGLGPALPATVVLDREGRVVERFDGLFERAALEDAIDRALDGGS
                     RAAVEPHVHVAAAAAGTGGASLVPS"
BASE COUNT         1270 a         2974 c         2899 g         1333 t
ORIGIN      
        1 cgagcggggt gggccgctcg tggtcgtcgc ggtgggtcag gttgagcaac ccgtagctga
       61 cgcgggccga cgtgccgtcc ggcgcgacgt cattcaggcg caccgcgatc atcgccaccg
      121 gggcgtccga cgacaggtcg agctcgacca ccggcgcgcc gaggatctcg acccgctcgg
      181 cgagcgcgtc accgtcgaag accagcgagc gaccgtcgtc cggccgctga tcgagcggca
      241 tctcgccgtc ggcgccgaac gcgcaccact cgcctgccgc cagccccgtg gtctgtggcg
      301 acgagaaggc gatcgtcgtc ggttgcgacg gcgcgtgctg tagccgcccc ggattgagcc
      361 ggaagcgttg gagctcgatc cgcggcgagg gccaactcgc ctcggcaacc cagcggccgg
      421 ggcgctcctc gtactgcggc gccggtggaa cgctctcctg catccagacg cgaaaacgcg
      481 gttcctccat gatcccggtg gttcgaccgc gcagccactc gtcccaccag cggaccgcct
      541 cctgcaggaa gccgatcggc ggacccgggt aaccggtgtg cggaaaggtg tgggcccacg
      601 ggccgatcaa ccccttgcac gggccgcgca agcctgcgag cagtcgaggc acggcgttgc
      661 tgtatccgtc ggcccagccg ccgatggcgt agaccgggca ctcgatcgcg tcgtagtcct
      721 ggcagaccga gccgtgtttc cagtaagcgt cgcgccacgg gtgacgcatc cagcgctcgg
      781 cgaacggcgc cgccccgtcg agccgatcga gccacatcgt ccgccagcgc tcgccgacga
      841 tctgcgggtc gggcggcagt gcgccgtagg tcatcaggat cgcgccccat tgcacgtttt
      901 cgttcagcaa gcagccgccc atatagtgcg cgtcgtcggc gtagcgatcg tcggtcgagc
      961 agagcgtgat caccgcgcgc aacgcgggtg gccgcagcgc ggcgacctgc agggcgttga
     1021 acccgcccca ggagataccc atcatcccca ccgagccgtc gcaccagggc tgccgcgcca
     1081 gccaatcgat gatctcgacg gcgtcggcgt gctcggcgcg cgtgtactcg tcggtcagca
     1141 cgccctcgct gtcgcccgag ccgcggatgt cgacccgcac ggccgcgtag ccgtgaccgg
     1201 cgaagtaccg gtggatcggc tcgtcgcgct cgcgcgtgaa gtcacgcttg cgatagggga
     1261 tgtactcgag gatcgccgga accgggttgc gttcgacgtc gtccggcatc cagatgcgag
     1321 ccgcgatgcg acaaccgtcg tgcagcggga tgaagacgtt ctcgatctcg cgaacgtgct
     1381 cgacgaggtc gtgccggacg atcggcccgc tcatcgctcc ggctcagccg ccgagtttga
     1441 cgccgacgcg gccgcgacgc gagaccaacc tctgcgtgct ccagaacgcg agggccaact
     1501 cctggcgcgc ggcgatgccg gcgtcgagat ccgacccgtt gatcacggcg agacggaagc
     1561 cgccaggcgg catcgagtgc gcccaggtcg gcttatcctc gtggaacgtg atctggatgc
     1621 tcgagatccg cgggtggtcg agcaaccgct cgacgagcag ccgatcgggg tggcggtact
     1681 gcacgccgtg cgcaccgaac agcacgacgc tgtatccgtc gcgccgatcg tcgtcggcgt
     1741 ggtcccacac gccgcgggcg tacaaccgca ccagcgcgtc gagccagccc ctgccgtaga
     1801 ggtcgggcca ctgatcggcg aagcgcaggt gggcctcgat gatccgagcg ccgatcgtct
     1861 cgagattgac gatcccggta tagtcgggca ggttccgcgc gatccactcg ccgcagtacc
     1921 gctcgatctc cggtcgagcc tcggcgagga tcgtccagta gtcgaacttg ccgtcgccga
     1981 gcgccttggc gagcacgtgg cgccaccagc gcgcctcgcc gcgcacgacg gcgacgtcgg
     2041 tgctgacgtg ctcgccctcg agcaactcca tccagatgtg gcccggccgc tggtagcgct
     2101 cgtactcctt cggcgagcgc aggattcggc taccgacgcc catgccgcgc atgttgtaga
     2161 ccggcttgct gaagacggga taggacggtg ggtcgatccc gtgcagtccg caggacagac
     2221 cctggctgtc ggcgatcagc agcttgttga acgtccagcg gtatcgcgga aacagcttcc
     2281 aggcgtcctg ctcctcggtc gggatgacga cgtcgtcggg gcagtcaagg ccgacgaagt
     2341 actgcatccg ccaggggtcg cgttcgatca gcggcatcga tcggctcact tcccgagctg
     2401 gtccttcagg tccttgacct tgtagcccgg tgggacctcg aacacggcgg cgtcgatctc
     2461 ccgacggttg atcgacgcga gcgtcgtctc gctgaccaac cgctccttgt cgtcgaaggt
     2521 gcggacgcga accgggaagc cgaggtcgtc catctcggtg tacggcgtgt cgatcatgcc
     2581 gacgaacgcg ctgtcgcgga cgacacgctg gagcgactcg gtgaaacggg acatcgcgcg
     2641 gaaggcgtcc atcgcttcga tcacgtccgc cgcgacgctc tcgtcggccg cgcagacctc
     2701 ccacaccttc ttctcgtccg agtaaagcgt gtgcacctcg caggtgtact cgccgatctg
     2761 ctcggtcccg ccggaatcga tccgtcggga cggcggcgcg gagactcccg gcatcttgcc
     2821 ctgcatcatc ttttccatca tcgcgcgctg ctcgggcggc atctgctcga actgcttcat
     2881 catctgatcg agccgagcgc tgagctgctc gactccctca cggtcgatct tctggcattg
     2941 cttcttgtcg tggtcgacga accacagcgt ctgatcgcgg aagatcgccg acatctcccc
     3001 gccgccgcgg gcccggtgcg ggtccatccg caccatctcg ccctcggcgt agaatctctc
     3061 gctgccgacc gcgtccggcg agtcgggcgt tcggacctcc atctcgacca cgacgccggc
     3121 gtgggccgca gtcgccacca gaccggcggc catccaccga acggcgaagc gtctcatcgg
     3181 tctccctcct tcagcgtcgt ccggcgatcg tacaccgaac cgcggcggcc ggccggtcgc
     3241 ggaccgtcgc gggcggaaat ccgacgatga ccggccgtcc ccttgcccgg ggaatcaccg
     3301 tcacggcagc gctggggctg gcggcggcaa gcgaggcggg gagggagatt cgacgagagg
     3361 caaggccggc ggcccgttcg cgccgccggc ccgggaaccg aaacctcggc agccctacga
     3421 cccgctgccc ggtttggacg aagccggctt gggtccggcc ggtttacccg tcatcggctt
     3481 cttgaccggc gagggggggg cagtggtctt gtttccgctc ttcggtttgt cggccatgtt
     3541 ctcaacctct acgggaccgc ttcgggtggc ccgcgtcgga caggcgtcgg gactgccgcc
     3601 tccgtcgtca cggtgaggtg caagagcgat gcctcgggtt ttcgctcgtg atcagacccg
     3661 gcggttccgt ttcctcgaca cgcgccggcg cgttcagaac ccgcgcgacc gatcggtcga
     3721 agcggcgtgg cataatccgg ccgagccacc gtgagcgccc cctcccatcc gcccgccgac
     3781 ctgcgcgaac aacgacatgc catgcggctg tcgctcgtcg tcgggctgtc gatgctgctc
     3841 gccaagtggt acgcgttctc gatcacgaac tccgcggcga tcctgtccga tgcggccgag
     3901 tcggtcgtcc acgtcgcggc cgtcgccttc gccgcgttca gcctgtggct cagcctgcgt
     3961 cctgccgatt cgagccacct ttacggtcac gacaagatca gcttcttttc cgccgggatg
     4021 gaaggcctcc tgatcgtgct cgccgcggtc tacatcgtct acgaggccgt cgcgcgctgg
     4081 atcgtcggac tcgagctgca caacctgggg accgggacga tcgtcgtcgg cgccgcggcc
     4141 gcagtcaatc tcggtctcgg tatctacctc gtccggcagg gacaacggca ccgctcgttg
     4201 atcctggtgg ccaacggcaa gcacgtgctc accgactcgt ggacgagcct cggtgtgatc
     4261 ggcgggctgt tgctcgtgct gtggagcggg tggcggccgt tcgatccgct gctcgcgatc
     4321 ctcgtcgcga tcaacatcct gtggtcgggc gggaagctgg tgcgagagtc gatcggcggg
     4381 ctgatggacg aaagcgacga ggagacgcac cgcgtgctgg tccggttgct cgaccgggag
     4441 acgaccggcc gcgggctgtc gtaccacgag ctgcgccacc gcaagagcgg tgccgggacg
     4501 tgggtcgagc tgcacctgct cttcccgcgc gagacgacga tcgaggcggc ccaccatcac
     4561 gcgaccaaga tcgaggccgc gatcgaggcg gagctacccg gcccggtgac cgtgacctcg
     4621 cacctcgagc cgcgagaacg gcacgacgag atccacgaca ggtcgcgccg ccgctgactc
     4681 agtcgccggt gggaacgatg gtgaagtcga gcacgtccgc cgattctctc aatcgctcgg
     4741 cgaggcgtga cgcgttggct ttgtggccgg tgttgatgac cgtgcggtac tcgaagacgg
     4801 taccgtcgtc gctcagcgca tagctcatgt tgcccgccac gaaccccaac tcgtgcaact
     4861 cgccgcggac cgtcacctcg gacggtacgc gcgtgttgcg gaagcgtacc gcgtaatgcg
     4921 cgtagtagcg ccaaggaatc cggccctcga cccagcggaa caccgccagg atcccgatcg
     4981 tcaacgcggt tccgagcacg gccggcccgt acatgccgat gccgaacagg atgccgaggg
     5041 cggcggtcgt ccagaccgat gccgccgtgg tcagaccgcg gacggtcagc ccttcccgga
     5101 agatcacacc gccgccgagg aagccgattc cggtcatgat gccctgcgcc atccgtgtcg
     5161 gatcgatgcg cagcatctcg gccggagcgt cgtccagcca gcggtagccg gtcagcagca
     5221 tcaacagggc ggaggcgatg cagaccaggg cgtgcgtgcg gaaaccggcc ggccgactgt
     5281 gaaacgaccg gtcgaggccg atcaggctgc cggccgccca ggcgctgccg actttgagcg
     5341 ccgtgtcgag caactccccg tgccacacga ccgacactct agcgcagttt cccccggtcg
     5401 aacggcgggt gcgatatcat tccggtgaga agcttggagc cgatgatgaa gaccatccaa
     5461 aacaagaccc acaaaccgct gcggatcccg ttggacggcg gccacgtgct gcacctcggc
     5521 ccgaacaaga ccgggcgggt atcggaccac gcggccgagc ggccggcgat tcggcgtctg
     5581 gtcgaaagcg gcgagatcgc gattctcgac gacggcggcg ataccggaga cgcgggcgcc
     5641 gagacggccg gcgggcccga ggtgccggcc gccgcccccc agcgcaaggt cgtcatcccg
     5701 accggcggtc gcaccgccgg gggacgccgc gaacgctgag ctcgacccgc ggcgcggcta
     5761 ttcgccgcta cgtcggcccc cgggtgtcga ccgcggcggg caggtccgag tcgaccagat
     5821 cgccgagttc ggcggaggtc gggttcgagc ggatcttgat cagcgcccgg tgctcgatct
     5881 gccggacccg ctcgcgcgac agacccatcg cctcgccgac cgactggagc gtcggtcgcc
     5941 gctcgccgtc cagaccgaag cgcagccgga tcaaccgttg ctcctgcggc gtgagccgct
     6001 cgagcgccgc ctcgacgacc cgccgccgct gcgcgctcag cacctgctcg aaaggacaga
     6061 cggcctgcgg gtcggacgcc gtccgcacga gatcgaacgt acgctccggg tcctcgagcg
     6121 ggtcgttgtc gcgcatcgag ccgagaatct cgtgcacctt cgaggtcgtc aagccgagct
     6181 cgcccgcaat ctcctccgtc gtcggctcac gccggcgccg ggcccgcagc gtctccgacg
     6241 cgcgccggat ccggcgggcc ttgtcctgaa cgtgcacggg aacgcgtacc gcgcgcgcct
     6301 ggttgccgat cgcgcgctcg atcgcctgct tgatccacca gtacgcatag gtcgagaagc
     6361 gattgccgcg ccggtggtcg tacttgtcga cggctttgat caagccgagg ttgccctcct
     6421 gcacgaggtc ggacagtgcc aggttcggat gggggtactt cttcgcgatg tggacgacga
     6481 gccgcaggtt ggcgcggatc agctgatcgc gcgcatcgtc gagccgccgt ttgtgccgcc
     6541 gcgtctcggc cagcagcttg gccgcgcccg tgtcgcgacg ggcgcggcag cggcggacca
     6601 agtcctcgac gaagcggtcg acctgctcga gcgtccacgg ctcgccccgt cgcgggcggg
     6661 agccgagcgt gctcgcgacg tcgcgcccga gcgtccgtcc ggctccgcgg gcgagcgcgc
     6721 gcagcgcccg ccgcgcgagg gccagtctcc gcgccaggtc gaactcgcgc tccgggagga
     6781 gcaacggcac gctacctgcc tccctcaggt agacggcgag ggagtcggcc gcagcacccc
     6841 cgtcgacgac cgtcgcggcg gtcttcggca ggccatcagg ctcctgtcgg cgccggtgca
     6901 ctcgtcctcc gaggatgaag acccaggaag tacctagcgt agggaattaa ccggccgatc
     6961 gcaacccgca gcgaggggtc gttccgttcg cgcgccggcg ctcggcctca gcggcggcgc
     7021 ggcctcgcgg gcggtcgggc gacgaacgcc gggaccatgc cgcggcgcag gtacgagccg
     7081 cgaacgctcg aaggcagctc ggaccagcgc ctgacgattc cgcggcagcc gttcgccccg
     7141 cactggcagg ggaaggtcgt cgggtcggtc agcatcgccg tcgagtagtc gaaggtcagc
     7201 tcttcgccac ggcggatcgg tcgcaccgcg cgcaacagcg cggcacggcc gttggtctcg
     7261 accgcgcaat tcgggtcaca actgtggttg accaggtcgt caagctcccc cgacggaccg
     7321 agaaaccggc cgggcgcgta ctccagcacg tgggtgaact cggtcacttc ttgaaacctc
     7381 agctcgctcc cgcggaaggc cagcacgaac tcgccgaccg cgatcgggcg gcgggcgacg
     7441 attccccgtc cgcgtcccgg cagctcgacc gcgcggcagg cggcagtcac cgtcacgatc
     7501 gacgcttccg cgctattcga catgcaccac ctcgagcttc accgccgcgc gcacgtcgta
     7561 caggtccgtg cgacactcga cgagcggacg caccgtgccg atctcgtcgg gcaacaccag
     7621 gaagcggcag gggtattcgt tggccgtgtc gccgaagcac ttgacgatct gctcgaactc
     7681 ctcgcggtag gccgccccaa ggcactcgac cagcgccggg atgtctttcg ttctccagcg
     7741 acggacccgg acgttggttt cgctcatgtt gctccgcggc ctgatttagc ccgctcgtcg
     7801 gtccatgtca agtcgtttgt ccgcgccgcg acgtgcatct cgcggccagc gtgttatggt
     7861 caccggatgc tacgactgac gatcggttcg ctgctcgtcg tgttgtcggc cgtcgccgtc
     7921 gccgccgaag caccggcgct gtcctggacc gacttgtcgg gccggcgctt ggcgctcgac
     7981 gacctgcgcg gccgggtcgt cgtgctgaac ttctgggcga cctggtgcga gccgtgccgc
     8041 cgggagctgc ccgagctggt gcgggtgcag acccgttacg gcgtcctcgg gctccaggtg
     8101 ctcggcgcgg gcgccgatcc gcccgctcaa agcgaggccg tgctgcgttt cgcgcggtcg
     8161 cggaagctga acttcccgct cgtcctcggc gccgacgcca agcagatgga ggcgctcggt
     8221 ctgggcccgg cgctcccggc gaccgtcgtg ctcgatcgcg agggacgcgt cgtcgagcgc
     8281 ttcgatggtc tgttcgagcg ggccgcgctc gaggacgcga tcgaccgcgc gctcgacggc
     8341 ggcagtcggg cggcggtcga gccgcacgtc cacgtcgccg ctgccgcggc cggcacgggc
     8401 ggggcctcgc tcgttcccag ctgaccggtc tgacccggcg agggtcgggc gggccggggg
     8461 accgccgggg gggaga
//
LOCUS       DASPGN010000287         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_167821, whole genome shotgun sequence.
ACCESSION   DASPGN010000287 DASPGN010000000
VERSION     DASPGN010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167821"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VD788_08410"
     CDS             <1..1160
                     /locus_tag="VD788_08410"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HXV76328.1"
                     /translation="DGRRVLMRVDFNVPIEDGEVADDSRIVAALPTIRAALDRDASVV
                     LMSHLGRPKGRVDRSYSLAPVRPVLARRLGREVAFAGDCVGAATESAAAALAAGGVLL
                     LENLRFHPGEEKPESEPGFADALARLGERYVNDAFGTAHRAHASMVAVAERFARPAAG
                     YLLARELAYFDRALSSPERPFLALLGGAKVGDKIPLIESLLERVDGILIGGAMAYTFL
                     VARGIAVGGSRVEQDKLELAGKLVEAARLREVDLLLPIDHLCGRTFDEATERRTVESA
                     EIPAGWIGLDIGPRTIEAYRDRLRGAGTIVWNGPMGVFEWQAFSEGTMAMAHALADSG
                     AVTIVGGGDSVAAANRSGLAERFSHISTGGGASLELLEGKELPGVAALADR"
     gene            1191..1952
                     /gene="tpiA"
                     /locus_tag="VD788_08415"
     CDS             1191..1952
                     /gene="tpiA"
                     /locus_tag="VD788_08415"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HXV76329.1"
                     /translation="MNRRPIVAANWKMNGALAEARALARGVLERLAGFTAADVVLCPP
                     FTALAEVARVIAGSPVALGAQHLHWERRGAFTGEVSAEMLRDVGCRFVLVGHSERRSL
                     FGEADATVRRRTEAALETELTPIVCVGEALAEREAGQTDTVLGTQLDRGLGGLGDALA
                     GVVIAYEPVWAIGTGRNATAAQAQSAHSFIRARLGSICGETLAAGIRILYGGSLKPDN
                     AAELCSQPDVDGGLVGGASLDAGSFVEIVRRAAGG"
     gene            complement(1963..>2451)
                     /locus_tag="VD788_08420"
     CDS             complement(1963..>2451)
                     /locus_tag="VD788_08420"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HXV76330.1"
                     /translation="SPIGELVVVTRGGVVCAIAFGRRYDGLERGLERRFARIDWSDAP
                     ADGAATTALGRYFDGDVRALEPLAADPGGSPFQRAVWDVLRRIPPGETRSYLDVARRV
                     GKPDAVRAVARANATNPVPLIIPCHRVIGADGSLTGFGGGLDRKRWLLRHEAAGVEFA
                     LR"
BASE COUNT          329 a          857 c          930 g          335 t
ORIGIN      
        1 tcgacgggcg tcgcgtgctg atgcgcgtcg acttcaacgt tccgatcgag gacggcgagg
       61 tcgcggacga cagccggatc gtcgccgcgc tgccgacgat ccgcgccgcg ctcgatcgcg
      121 acgcctcggt cgtgttgatg agccacctcg gccggccgaa gggtcgagtc gatcggtcct
      181 acagcctggc accggtccgg ccggtgctgg cccgccggct gggccgcgag gtcgccttcg
      241 ccggcgactg tgtcggagcc gcgaccgaga gcgcggccgc cgcgctcgcc gccggcgggg
      301 tcttgctgct cgagaacctg cgcttccacc cgggcgagga aaagccggag agcgaaccgg
      361 ggttcgccga cgcgctggcc cggttgggcg agcgctacgt gaacgacgcc ttcggcaccg
      421 cgcatcgcgc gcacgcgtcg atggtcgccg tcgccgaacg cttcgcgcga cccgccgcgg
      481 gctacctgct ggccagagag ctcgcgtact tcgaccgcgc actgtcgtcg ccggagcggc
      541 cgttcctcgc gttgctcggc ggcgccaagg tcggcgacaa gatcccgctg atcgagagcc
      601 tgctcgagcg ggtcgacggg atcctgatcg gcggtgcgat ggcctacacc ttcctcgtcg
      661 cccgcggtat cgcggtcggc ggctcgcgcg tcgagcagga caagctcgag ctggccggca
      721 agctggtcga ggccgcgcgg ctccgcgagg tcgacctgct gctgccgatc gaccatctgt
      781 gcggccggac gttcgacgag gcgaccgagc gtcggaccgt cgagtccgcc gagatcccgg
      841 cgggttggat cgggctcgat atcggcccgc gcacgatcga ggcctaccgc gaccggctgc
      901 gcggcgcggg gacgatcgtc tggaacgggc cgatgggcgt gttcgagtgg caggcgttct
      961 ccgaggggac gatggcgatg gcccacgcgc tcgcggacag cggcgccgtg acgatcgtcg
     1021 gcggcggcga ctcggtcgcg gcggcgaacc gatcggggct ggccgaacgc ttcagccaca
     1081 tctcgacggg cggcggagcg agcctggagc tgctcgaggg caaggagctg cccggggtcg
     1141 cggcgctcgc cgaccgttga tcgaccaccg cccgtgctcg gagtgaagcc gtgaatcgac
     1201 gcccgatcgt cgccgcgaac tggaagatga acggcgcgct ggccgaggcg cgggcattgg
     1261 cccgcggggt gctcgagcgg ctcgccggtt tcaccgcggc cgacgtggtg ttgtgccctc
     1321 cgttcaccgc gctcgccgag gtcgcgcggg tgatcgccgg gagcccggtc gccctcggcg
     1381 cgcagcacct ccattgggag cggcgcggcg ccttcaccgg cgaggtgtcg gccgagatgc
     1441 tgcgcgacgt cggctgccgt ttcgtgctgg tcgggcacag cgagcgccgc agcttgttcg
     1501 gcgaggccga cgccaccgtg cgccgtcgca ccgaggcggc gctcgagacc gagctgacgc
     1561 cgatcgtctg cgtcggcgag gcgctcgccg agcgcgaggc ggggcagacc gacacggtcc
     1621 tcggaaccca gctcgaccga gggctcggcg gcttggggga cgcactggcc ggggtcgtca
     1681 tcgcctacga gccggtgtgg gccatcggaa ccgggcggaa cgcgacggcc gcacaggcgc
     1741 agtcggccca ctccttcatc cgcgcgaggc tcggctcgat ctgcggcgag acgctcgcgg
     1801 ccgggatccg catcctgtac ggcggcagcc tcaagcccga caacgcggcg gagctgtgct
     1861 cgcaaccgga cgtcgacggc gggttggtcg gtggcgcgtc gctcgatgcc ggatcgttcg
     1921 tcgagatcgt ccgccgcgcg gccggcggtt gagtcacggc catcagcgca gcgcgaattc
     1981 gacgccagcc gcctcgtgac gcaacaacca gcgcttgcgg tcgagaccac cgccgaagcc
     2041 ggtcagcgag ccgtcggcac cgatcacgcg gtggcacggg atgatcagcg gaaccgggtt
     2101 ggtcgcgttg gcgcgagcca cggcgcggac cgcgtcgggc ttccccactc ggcgcgcgac
     2161 gtcgagatag gaacgggtct caccgggtgg aatgcggcgc agcacgtccc acaccgcgcg
     2221 ctggaacggg ctgccgccgg ggtcggcggc gagcggttcg agcgcgcgga cgtcgccgtc
     2281 gaaatagcgg ccgagagcgg tcgtcgcggc gccgtccgcc ggagcgtcgg accaatcgat
     2341 ccgggcgaag cgccgttcga gccctcgctc caggccgtcg tatcgtcgcc cgaacgcgat
     2401 cgcgcagacc acgccgccgc gcgtgacgac gaccagctct ccgatcggtg a
//
LOCUS       DASPGN010000288         2602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1455539, whole genome shotgun sequence.
ACCESSION   DASPGN010000288 DASPGN010000000
VERSION     DASPGN010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455539"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VD788_08425"
     CDS             complement(<1..987)
                     /locus_tag="VD788_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76331.1"
                     /translation="MRPPPALWRAIAIPVLISTAAGAGAPPILTASDVEPTLLDSLSI
                     DGELLAAAVSPSGERAALAYRSDDPKTERSLLRLLALDREPPATVELPGLVRDLLFAV
                     DGSGTFVIVFKPVRREQGRSALMWIGFDDPRPSNVLRLPPTAASLDYWAADRSLIVSA
                     RNELSTIRLDGLRSGRLFRVPGDNHSVVTLGSSSLVLVGQADRILLIDLDDPPGEEEI
                     APRDSVPVDAPVIELAAAPNGAHALARLADGRAYRVRPWPLELTALNPASAVVAAGKT
                     GDRRPFDPPPPAPGSDATATDGARVTTTAERVATPPVERSATPTSPPSETGVA"
     gene            1079..2002
                     /locus_tag="VD788_08430"
     CDS             1079..2002
                     /locus_tag="VD788_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV76332.1"
                     /translation="MHELALRSIGLCKRYDDVIAVDGLDLEVWRGECFALLGPNGAGK
                     TTTVEIFEGLLKPDAGDVEVLGSRWQTAGRALRERLGIQLQETQLADKISVLETIRLF
                     RSFYRSGPTPGEVLEMVGLGEKRDAWVRKLSGGQKQRLSLACALVGDPDVLFLDEPTT
                     GLDPQSRRRIWQVVAALRARGRTVLLTTHYMEEAERLCDRVAIIDRGRVIALGTPREL
                     IASLGAEHVIEFESSRPVEVDLLRSLPGIRDVAEENGAMRLTVSQVHQSIPALLAELA
                     RREIELTRLATHHATLEDVFVSMTGRHLRDE"
     gene            2011..>2602
                     /locus_tag="VD788_08435"
     CDS             2011..>2602
                     /locus_tag="VD788_08435"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV76333.1"
                     /translation="MYRPRPMVELTLTKIRELVREPEALFWIFVFPILLALALGIAFR
                     GAGDDALAVAVQRGDGAEWVRRSLELAGGFRMELVDEAVARRALRKGDVALVVIPGDR
                     WTYWFDPTRPESRLARLAVDDALQTAAGREPARGTVDRELSEPGSRYIDFLIPGLLGM
                     NLMGTGMWGIGFYVVNARSKRLLKRLTATPMKKTEYL"
BASE COUNT          383 a          872 c          964 g          383 t
ORIGIN      
        1 ggcgaccccc gtttcggacg gcggggaggt cggcgtcgcg ctccgctcta cgggcggcgt
       61 tgcgactcgt tccgccgtcg tcgtcacacg cgctccgtcc gtcgcggtgg cgtcggatcc
      121 cggcgccggc gggggcgggt cgaacggtcg tcggtcgccc gtcttgcccg cggcgaccac
      181 cgcggaggcc gggttcaacg cggtcagctc gagcggccag ggccgcacgc ggtaggcacg
      241 gccgtccgcc agccgtgcca gcgcgtgggc gccgttcggc gccgccgcca gctcgatcac
      301 cggcgcgtcg accggcacgc tgtcgcgcgg ggcgatctcc tcctcgcctg gcggatcgtc
      361 gagatcgatc aacaagatcc ggtcggcctg gccgacgagc accagactgc tcgagccgag
      421 cgtgacgacc gagtggttgt cgcccggaac gcggaacagc cggccgctgc gtagcccgtc
      481 cagccggatc gtcgacagct cgttgcgcgc cgagacgatc aacgatcggt cggcggccca
      541 gtagtcgagc gaggccgccg tcggcggcag tcgcaggacg ttcgacggcc gcgggtcgtc
      601 gaacccgatc cacatcagcg ccgagcgccc ctgctcgcga cgcaccggtt tgaacacgat
      661 gacgaacgtg ccggacccat cgacggcgaa caacaggtcg cgcacgagac ccggaagctc
      721 gacggtcgcc ggtggctcgc ggtcgagggc gaggagcctc aacagcgagc gctccgtctt
      781 cgggtcgtcc gagcggtagg ccagcgccgc gcgttcgccc gacgggctga cggccgcggc
      841 cagcaactcg ccgtcgatcg acagcgaatc gagcagcgtc ggctcgacgt cggaggcggt
      901 gaggatcggc ggcgcgcccg cgccggcggc ggtcgagatc agcaccggga tcgcgatcgc
      961 gcgccacagg gccgggggcg gtcgcatcgt cacctccgcc accgaggatg ccagtctttg
     1021 cgacgggtcg caacggcgcg ccgcgggaag cggctgccat ctgggctatc atgcggcgat
     1081 gcacgagctg gccttgcgat cgatcggcct ctgcaagcga tacgacgacg tgatcgcggt
     1141 cgacgggctc gacctcgagg tgtggcgcgg cgagtgtttc gcgctgctcg gtccgaacgg
     1201 cgccggcaag acgacgacgg tcgagatctt cgagggattg ctgaagccgg acgccggcga
     1261 cgtcgaggtg ctggggtccc gctggcaaac cgcgggacga gcgctacgcg agcggttggg
     1321 aatccagctc caggaaaccc agctggccga caagatcagc gtgctggaga cgattcggct
     1381 gtttcgcagc ttctaccgct cggggccgac accgggcgag gtgctcgaga tggtcggcct
     1441 cggcgagaag cgcgatgcct gggtgcgcaa gctgtccggc gggcagaagc agcggctgtc
     1501 gctggcctgc gcgctggtcg gtgatcccga cgtgctgttc ctcgacgagc cgacgaccgg
     1561 tctcgacccg caatcccgcc ggcgaatctg gcaggtcgtc gccgcgttgc gcgcgcgggg
     1621 tcgcaccgtg ctactgacga cgcactacat ggaggaagcc gagcggctgt gcgaccgggt
     1681 ggcgatcatc gaccgcggtc gcgtgatcgc gctcggaacc ccgcgcgagc tgatcgcctc
     1741 gctcggcgcc gagcacgtga tcgagttcga gagcagccgc ccggtcgagg tcgacctgct
     1801 gcggtcgctg cccgggatcc gcgacgtcgc cgaggagaac ggggcgatgc ggctgacggt
     1861 gtcgcaggtc caccagtcga ttcccgcgct gctcgccgag ctggcgcggc gcgagatcga
     1921 gctgacccgg ctggccaccc atcacgcgac gctcgaggac gtcttcgtct cgatgaccgg
     1981 tcgccacctg cgcgacgaat agcggcgacg atgtaccgac cccggccgat ggtcgaattg
     2041 accttgacca agatccgcga gctggttcgc gagcccgaag cgctgttctg gatcttcgtc
     2101 tttccgatcc tgctcgcgct ggcgctcggc atcgcctttc gtggggccgg ggatgatgca
     2161 ttggccgtcg cggtccagcg cggcgacggc gcggagtggg tccgccgcag cctggagctg
     2221 gccgggggat ttcgcatgga gctggtcgac gaggccgtcg cgcgccgggc gctgcgcaag
     2281 ggcgacgtgg cgctggtcgt gatcccgggc gaccgctgga cgtactggtt cgacccgacc
     2341 cgaccggaaa gcaggttggc gcggctggcg gtcgacgacg cgttgcagac cgcggcgggg
     2401 cgggagcccg cgcgcggcac cgtcgatcgc gagctgagcg agccgggatc gcgttatatc
     2461 gacttcttga taccgggtct gctcggcatg aacctgatgg gcaccgggat gtggggaatc
     2521 ggcttctacg tggtcaacgc gcgatccaaa cggttgctca agcggttgac cgccaccccg
     2581 atgaagaaga ccgaatacct gc
//
LOCUS       DASPGN010000289         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1222768, whole genome shotgun sequence.
ACCESSION   DASPGN010000289 DASPGN010000000
VERSION     DASPGN010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222768"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VD788_08440"
     CDS             complement(<1..654)
                     /locus_tag="VD788_08440"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HXV76334.1"
                     /translation="MRSYHDISGDGGSSVVGQISAQRERIRHKLAGVRWRVAIGSGKG
                     GVGKSTLTYQLASALAADGRRVAILDADINGPTQARLAGLRDAPLVPGDNGLVVPRAA
                     DGCPGVVSLGGLVPETESIEFDAVSSGESHTWRATREFALLAQLLESVDWGRLDMLWI
                     DLPPGAERSFQYAEFLGPETAFVIVGLPSELARGVVSRSVAALRRTPNPLIGYIENMR
                     "
     gene            complement(777..1634)
                     /locus_tag="VD788_08445"
     CDS             complement(777..1634)
                     /locus_tag="VD788_08445"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV76335.1"
                     /translation="MAGYRFCRSDDLAELVAAYASCYRVHLAECPVLTLDELKHWIRD
                     YGVWASSCMLAYSDRGEVVGVLIGAKRENDNGILAVGVRADQQRAGHGSHMLTSLANK
                     LAILGPPTIVAEVPETAVAARACFESLGYVAWRSYTDFRLTAGSGPAAQMHPLVGTID
                     VERAGEAGLLEGRDVCWNRASESLLRRSAVISGLALASDRRIEACLLYSTSDDGRRAR
                     IELIHLPDKTRGAPLLGLVAGQLLASGPSVVELPRAHEQEVPFDLLRTVGFRATGSTI
                     GYRVRAGSG"
     gene            complement(1647..>2154)
                     /locus_tag="VD788_08450"
     CDS             complement(1647..>2154)
                     /locus_tag="VD788_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HXV76336.1"
                     /translation="LPAAEEGAAEERPLEPGLRLIARDAKHNPLISALDWSSEAVERL
                     FRVPAGYMRDQTQRRTETIAEENGLPRIELATVEQGIEIGRRLMDELLSGQTLAARPA
                     AAAATDRPDASKCPFSGIYTGGVVPSEHRSPGDGDAADSDGENGEPYLNEIGILSAMA
                     ERRAKLEK"
BASE COUNT          326 a          799 c          708 g          321 t
ORIGIN      
        1 ccgcatgttc tcgatgtagc cgatcagcgg gttgggcgtc cgcctcagcg cggcgacgga
       61 gcgtgagacg acgccgcggg cgagctccga cggtagaccg acgatgacga aggcggtttc
      121 cggaccgagg aactccgcgt actggaacga gcgttcggca cccggcggca ggtcgatcca
      181 caacatgtcg agtcggcccc agtcgaccga ctcgagcagc tgagccagca aggcgaactc
      241 cctcgtcgca cgccaggtgt gggactcgcc gctcgatacg gcgtcgaact cgatcgactc
      301 ggtctcgggc acgagaccgc cgagcgagac cacgcccggg cagccgtcgg cggcgcgtgg
      361 cacgacgagg ccattgtctc ccggcaccag cggtgcgtcc cgcaggcccg ccagccgggc
      421 ctgcgtcggc ccgttgatgt cggcatcgag gatcgcgacc cgccggccgt ccgccgccag
      481 cgccgacgcg agctggtagg tcagcgtgct cttgccgacg ccacccttgc ccgatccgat
      541 cgcgacccgc cagcgcaccc cggccagctt gtgccggatc cgctcgcgtt gcgcggagat
      601 ctgaccgacg accgacgagc cgccgtctcc cgagatgtcg tgataggaac gcatcgactg
      661 gaccgctatt ctgaccggtt cgcccgcgcg agacaagcgg tccgccgccg accccggttc
      721 cgccgccgcg gcccgacggc cgttgcccga tccgtgcctc atccgcgcgc cgcgtctcaa
      781 ccggaaccgg cgcggacccg gtacccgatc gtcgagcccg tcgcgcggaa gccgaccgtt
      841 ctcaacaggt cgaacggcac ctcttgctcg tgtgcgcgcg gcagctcgac gacgctcggg
      901 cccgaggcca agagctgccc cgcgaccagg cccaacagcg gcgcgccgcg cgtcttgtcc
      961 ggcaggtgaa tcagctcgat ccgggcgcga cgaccgtcgt ccgacgtcga gtagagcagg
     1021 cacgcttcga ttcgtctgtc ggatgccagg gccagccccg agatcacggc gctgcgccgg
     1081 agcagactct cgctggcgcg gttccaacag acgtcgcgac cctcaagcag ccccgcctca
     1141 ccggctcgct cgacgtcgat cgtaccgacg agtgggtgca tctgcgcggc cggtccgctc
     1201 ccggccgtca agcggaagtc cgtgtagctg cgccacgcaa cgtagcccag cgattcgaag
     1261 cacgcccggg ccgcgaccgc ggtctccgga acctcggcga cgatcgtcgg cggaccgagg
     1321 atcgccagct tgttcgccag cgaagtcagc atgtgcgagc cgtgaccggc gcgttgctgg
     1381 tcggcgcgaa caccgacggc gaggatgccg ttgtcgttct cccgctttgc accgatcagt
     1441 acgccgacca cctctccacg gtcggagtac gcgagcatgc acgagctggc ccacacaccg
     1501 tagtcgcgga tccagtgttt gagctcgtcg agtgtcagca ccggacactc ggcgaggtga
     1561 acgcggtagc acgacgcgta ggccgcgacg agctccgcta ggtcgtcgga gcggcagaat
     1621 cgatatccgg ccatacgccg gtcccgctac ttctcgagct tggccctgcg ctccgccatc
     1681 gcggacagga tcccgatctc gttcaggtac ggctcaccgt tttcgccgtc gctgtcggcc
     1741 gcgtcgccat cgcccggtga acgatgctcg ctcgggacga caccaccggt gtagatgccg
     1801 ctgaacgggc acttggaggc atcgggccgg tcagtcgcgg cggccgccgc cggtctggca
     1861 gccaacgtct gcccgctgag cagctcgtcc atcaggcgcc gaccgatctc gatcccttgc
     1921 tcgaccgtcg ccaactcgat ccgcggcagc ccgttttcct cggcgatcgt ctcggtcctg
     1981 cgctgggtct ggtcacgcat gtagccggcg gggacgcgga acaaccgctc cacggcctcg
     2041 ctcgaccaat cgagggccga gatcagcggg ttgtgcttcg cgtcccgcgc gatcaggcgc
     2101 agccccggct cgagcggccg ctcctcggcc gccccctcct ccgccgccgg cagc
//
LOCUS       DASPGN010000290         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_988632, whole genome shotgun sequence.
ACCESSION   DASPGN010000290 DASPGN010000000
VERSION     DASPGN010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_988632"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VD788_08455"
     CDS             complement(<1..795)
                     /locus_tag="VD788_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76337.1"
                     /translation="MTGLWLAVAGAADATEERFMLLSAETPERLERRLVELGDAGYRL
                     LGAATGVSAGGADRIAVLLESTAGSTDFDHAVVACDGRLESAGGPGIAHYASAGYRLL
                     PAGVVVRRMEDFWLPESSYDDQMVLILERSSGLSASYHYESVAFGSFERFHRSLADKQ
                     AGGFTLLGVWNSGRRLQALLERSSTAEPPPVPSGTSYRLLIQATRSGLRRAVRRAAGD
                     GFRVVAAADPSIAGPPIVLLGKTGVPADDVTYRFVADVPKKLHKDKL"
     gene            complement(825..2675)
                     /locus_tag="VD788_08460"
     CDS             complement(825..2675)
                     /locus_tag="VD788_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV76338.1"
                     /translation="MKAAAISSLLSVGIVFAPAVAQDVAPADPLELGLEESVEVQFVL
                     VDFLVVDRQGRTVPGLEADDFLLLVDGELVEIATLDAECPTGASADPRAGEAVEPDPS
                     PGEGPRQMVLVFDYYHMTGIADTVERVLAMIDEWPDGDDRHMIASLGEVVRLETPFTS
                     DRDELRWALRRMRNDRDLYAGQYGRLTERRFFDRMRVLFDLMERLPGRKSIVLFSGPF
                     MPDGFHHDPEYKALSGLSASARTSVYPVDVGGLRTPGDQGFGALGGPGELRRLANETG
                     GRMTADTNDITLAYARARRDLGCSYTLGFYDRPPRQDKNRRLTIRVTDRRGLRVVYPE
                     YYVVRSPEKKRASLLRTAAMAPHMFDSDQLDVELFVLGPQSTSRWRTVFGVEIRLGPD
                     EIVGEGETWELEAFLRKPNGTIIRSFKREIPMPATDPTTGRNPTVELFHEIGAPPGRY
                     VASVVLSDPDGAVPRATTRSATLAEIPRGDPFLVGPILGRQVPAVANGVAGEEEPPFR
                     PLIDGSTEAGQPLDALTVLCVRETQTEQTTHTVMRSLTAIDGSAVRRFDDVAVELSGA
                     GGVRCRELIDTLSTDLAPGRYEVRAHAESRGFVSETGTAGLTVEPAAPRR"
BASE COUNT          447 a          998 c          939 g          434 t
ORIGIN      
        1 gagcttgtcc ttgtgcagct tcttcggaac gtcggcgacg aagcgatagg tcacgtcgtc
       61 ggccggcacg ccggtcttgc ccaaaagcac gatcggcggt ccggcgattg acgggtccgc
      121 ggctgcgacg acgcgaaaac cgtcgccggc ggcacgacgc accgcccgcc gcagaccgct
      181 gcgggtcgct tggatcagca gccggtagga ggtccccgac ggaacgggtg gcggttccgc
      241 ggtcgacgat cgctcgagca gcgcctgcaa ccgacggccg ctgttccaca cgccgagcaa
      301 ggtgaaccca ccggcctgct tgtccgccag cgaccggtgg aaccgctcga acgagccgaa
      361 cgccaccgat tcgtagtggt acgatgccga caaccccgac gatcgttcga ggatcagcac
      421 catctggtcg tcgtagctcg attcgggcag ccagaagtct tccatccgtc gcacgacgac
      481 gccggccgga aggagccgat aacccgccga ggcgtagtgt gcgattcctg gtccgccggc
      541 cgattcgagc cggccatcgc aggcgacgac ggcgtggtcg aagtcggtcg agcccgcggt
      601 cgactcgagc aggacggcga tccggtcggc tccgccggcg gacactcccg tcgcggcccc
      661 gagcaatcga tagccggcat cgccgagctc gacgagacgc cgctcgagcc gctcgggggt
      721 ctcggccgac agcagcatga accgttcttc cgtcgcatcg gccgccccgg cgacagcgag
      781 ccacagtccc gtcaccgccg cgcttgcgcg cggacgccgg cccctcatcg ccgaggcgcg
      841 gccggctcga ccgtcagccc cgccgttccg gtctcgctga cgaagcctcg gctctcggcg
      901 tgggctcgaa cctcgtaccg accgggggcg aggtcggtcg acagcgtgtc gatcagttcg
      961 cgacagcgca cgcctcccgc tccggagagc tcgacggcga cgtcgtcgaa gcgccggacg
     1021 gcggacccgt cgatcgccgt caacgatcgc atcacggtgt gagtggtctg ctcggtctgg
     1081 gtctctctca cgcagagaac ggtcagcgcg tcgagcggct ggccggcttc ggtcgagccg
     1141 tcgatcagcg gccggaacgg cggctcctcc tcgcccgcaa cgccgttcgc gaccgcaggc
     1201 acctgacggc cgaggatcgg gccgacgagg aacgggtcac cgcgcgggat ctcggccaac
     1261 gtcgccgagc gcgtcgtcgc ccgggggacc gcaccatccg gatcggacag cacgacgctc
     1321 gcgacgtagc gcccgggcgg cgcgccgatc tcgtggaaca gctcgaccgt ggggttccga
     1381 ccggtggtcg gatcggtcgc tggcatggga atctcgcgct tgaacgagcg gatgatcgtg
     1441 ccgttcggct tgcgtaggaa ggcctccagc tcccacgtct cgccctcccc gacgatctcg
     1501 tcgggaccga ggcggatttc gacgccgaag acggtccgcc agcgcgaggt cgattggggt
     1561 cccaggacga acagctccac gtcgagctga tcgctgtcga acatgtgcgg cgccatcgcc
     1621 gccgttcgga gcaacgacgc ccgtttcttt tcgggcgatc gcacgacgta gtactccgga
     1681 tagacgacac gcaatccgcg gcgatcggtg acgcggatgg tgagacgacg gttcttgtcc
     1741 tggcgcgggg gtcggtcgta gaagcccaag gtgtaggagc aaccgaggtc gcgccgagca
     1801 cgtgcatagg ccaaggtaat atcgttcgtg tctgcggtca tccttccgcc ggtctcgttc
     1861 gccaggcgtc tcagctcccc gggcccgccc agcgctccga acccctgatc gccgggcgtt
     1921 cgcagcccgc cgacatcgac cgggtagacc gacgtgcggg ccgacgccga cagtccgctg
     1981 agcgccttgt actcggggtc gtgatggaag ccgtccggca tgaacggccc ggagaacagc
     2041 acgatcgact tacggcccgg cagtcgttcc atcagatcga acaacacacg catgcggtcg
     2101 aagaaccgtc gctcggtcaa ccgcccgtat tggccggcgt acaggtcgcg gtcgtttcgc
     2161 atgcgtcgaa gcgcccagcg caactcgtcc cgatcgctgg tgaacggcgt ctcgagccgc
     2221 accacctcgc ccaacgaggc gatcatgtgc ctgtcgtcgc catccggcca ctcgtcgatc
     2281 atcgccagca cccgttccac cgtgtccgcg atgccggtca tgtggtagta gtcgaagacc
     2341 aggaccattt gccgcggtcc ctcgccgggt gacgggtcgg gctcgaccgc ctcccccgct
     2401 cgggggtcgg ccgacgcccc ggtcgggcac tcggcgtcga gtgtcgcgat ctcgaccagt
     2461 tcgccgtcga ccagcagcag gaagtcgtcc gcctcgagac cgggcacggt cctcccttga
     2521 cgatcgacga caaggaagtc gacgagcacg aactggacct cgaccgactc ttcgagtccg
     2581 agctcgagcg ggtccgcggg ggcaacgtct tgtgcgacgg ccggcgcgaa cacgattccg
     2641 acggaaagca gggacgagat cgcggccgct ttcacccatg cccccttttc cggtcgttta
     2701 cgccgacgga agtcgccgcg ccgcccgtcg ggccggcgtc cgcggcgttt gccccgcacc
     2761 gccgggatcg gtcggtctct cccccgcatt ctatcgcgta agctcccgac gatctgcc
//
LOCUS       DASPGN010000291         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_288191, whole genome shotgun sequence.
ACCESSION   DASPGN010000291 DASPGN010000000
VERSION     DASPGN010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288191"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /gene="lepA"
                     /locus_tag="VD788_08465"
     CDS             complement(<1..1172)
                     /gene="lepA"
                     /locus_tag="VD788_08465"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXV76339.1"
                     /translation="MIGRFGGGFVSYIRNFCIIAHIDHGKSTLADRLIQSCGAVDPRR
                     FRDQILDSMEIERERGITIKSNTITLDYHAADGRQYQLNLIDTPGHVDFSHEVRRSLM
                     SCEGAILLIDASQGVEAQTVANMYLALEYDLELLPVINKIDLPSADVDRVLTEIESDL
                     GLDPSQAVCCSAKTGAGVADVLEAVVRRLPGPVVDRDAPTRAMLFDAQYDPYRGVVLL
                     CRVKDGVLRPGQKMLLMHSGKQHAIEEVGQLRLDRFPTAELASGSVGYVLAGIKTVRD
                     VTVGDTLTVVGQAAGEPLAGYKEAKPVVFSSIYPISTDEYPELAKALDKLVLNDAALT
                     YEKDSSVALGHGFRCGFLGLLHLEVVQERLRREYDLSLLLSAPSVRYKITLSGGEV"
     gene            1299..>2462
                     /locus_tag="VD788_08470"
     CDS             1299..>2462
                     /locus_tag="VD788_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV76340.1"
                     /translation="MQGSFRSVPLVALLAGRLAAAAAGADPIARADVDERVGARLYSV
                     RLAIRPTWTAGPGACLELDERDLRVRLRGDRVPPERIELDRERQPTLHALLIDTSDSV
                     AGDLDHIRRAATGYVERLDPGRDRAMIVTFDDSVLLHQPPTRDRHRLRQSIERLRTGA
                     GTSLHDGLYYVVRELSVQRERPVIILLSDGFDTSSVHERGDVLDVLDLRPDLTVFSIG
                     FDLPPMGTTGPTGMLSIRKFLQQLAGRTDGRFFDAPTGGTLDDVYRRIHETLAGEAVL
                     RVDDPEPDLSPGELRVGVGRDGCRVEVYRDREAPDDPLGVALSRPYADLPAAFELPPD
                     PRYRQTAIYRAFHRADPACRTGETDRWEAESDWRGIRGCVFDVAMDTGALYDPF"
BASE COUNT          393 a          859 c          823 g          387 t
ORIGIN      
        1 tcgacctcgc cgccggacag cgtgatcttg tagcgcaccg acggcgcgga cagcagcagc
       61 gacaggtcgt actcgcgccg caggcgttcc tgaaccactt cgaggtgcag cagcccgagg
      121 aaaccgcagc gaaagccgtg gcccaacgcg accgaggagt ccttctcgta ggtcaacgcc
      181 gcgtcgttca gcaccagctt gtcgagcgcc ttggccagct cggggtactc gtcggtcgag
      241 atcgggtaga tcgacgagaa gacgaccggt ttcgcttcct tgtacccggc cagcggctcg
      301 ccggctgcct gaccgacgac ggtcagcgta tcgccgacgg tcacgtcacg gacggtcttg
      361 atgccggcca gcacgtagcc gaccgatccc gaggccagct ccgccgtcgg aaagcggtcc
      421 agccgaagct gaccgacttc ctcgatcgca tgctgcttac cgctgtgcat cagcagcatc
      481 ttctggcccg gtcgcaacac accgtccttc acacggcaca gcagcaccac gccgcgatag
      541 ggatcgtact gcgcgtcgaa cagcatcgcg cgggtcggtg cgtcgcggtc gacgacaggc
      601 cccggcagcc tgcgcacgac ggcctcgagc acgtcggcga cgccggcacc ggtcttggct
      661 gagcagcaga ccgcctggct cggatcgaga ccgaggtccg actcgatctc ggtcagcacg
      721 cggtcgacgt cggccgacgg cagatcgatt ttgttgatga ccggcagcag ctcgagatcg
      781 tactcgagcg ccaggtacat gttggccacc gtctgcgcct cgacgccctg cgaggcgtcg
      841 atcagcagga tcgcaccttc gcacgacatg agcgagcgcc gcacctcgtg cgagaagtcg
      901 acgtgccccg gcgtgtcgat cagattgagc tggtactgcc gtccgtcggc ggcgtggtag
      961 tccagcgtga tggtgttgct cttgatcgtg ataccgcgct cgcgttcgat ctccatcgaa
     1021 tcgaggatct ggtcgcggaa ccgccgcggg tcgaccgcgc cgcaactctg gatcagacgg
     1081 tcggcgagcg tcgacttgcc gtggtcgatg tgcgcgatga tacagaagtt gcggatatag
     1141 ctcacgaacc cgcccccgaa tcgaccgatc attctgacac aaggcggctc gccggaccgc
     1201 acgcggctcg gtggcgatgc agcagctcgg agcgtcggtg gtagacatcg ccgccaaacc
     1261 gacgtacggt cctaaccggg taacggaggg gcggcccgat gcagggttct ttccgtagcg
     1321 tgccgctcgt cgcgctgctc gccggccggc tggccgcggc ggcggccgga gccgatccga
     1381 tcgcgcgcgc cgacgtcgac gagcgcgtcg gcgcccgcct gtacagcgtg cgcctggcga
     1441 tccgaccgac ctggacggcc ggaccggggg cgtgcctcga gctggacgag cgcgacctcc
     1501 gagtccgctt gcgtggcgac cgcgttccgc ccgagcggat cgagctcgac cgcgagcgcc
     1561 agccgacgct gcacgcgctg ctgatcgaca cgagcgacag cgtcgccggc gacctcgatc
     1621 acatccgccg cgccgccacc ggctacgtcg aacgactgga ccccggccga gatcgtgcga
     1681 tgatcgtcac cttcgacgat tcggtcctgc tccatcaacc cccgacgcgg gatcgccacc
     1741 ggttgcggca atcgatcgag cggctgcgca cgggagcggg aacgtcgttg cacgacgggc
     1801 tgtactacgt cgtgcgcgaa ctgagcgtcc agcgtgagcg accggtcatc atcctgctgt
     1861 ccgacggctt cgacacgtcg agcgtccacg agcggggcga cgtgctcgac gtgctcgacc
     1921 tgcgccccga cctgaccgtg ttctcgatcg gcttcgactt gccgccgatg ggcacgaccg
     1981 gcccgaccgg catgctgtcg atccgcaagt tcctgcagca gttggccgga cggaccgacg
     2041 gccggttctt cgacgccccg acggggggta cgctggacga cgtctaccgt cggatccacg
     2101 agacgctggc cggcgaagcc gtgctgcgcg tcgacgatcc cgagccggac ctctccccgg
     2161 gcgagctgcg cgtcggcgtc ggccgcgacg gatgtcgcgt cgaggtctac cgggaccgcg
     2221 aggcaccgga cgatccgctg ggcgtggctt tgtccagacc ctatgccgac ctgcccgcgg
     2281 ccttcgagct gccgcccgat ccacgctatc gccaaacggc gatctatcgc gcgttccatc
     2341 gagccgaccc ggcgtgccgc accggcgaga cggaccgctg ggaggccgag agcgattggc
     2401 gtggcatccg cggatgcgtc ttcgacgtcg cgatggacac cggtgcgctg tacgacccgt
     2461 tc
//
LOCUS       DASPGN010000292         6943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1339958, whole genome shotgun sequence.
ACCESSION   DASPGN010000292 DASPGN010000000
VERSION     DASPGN010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6943
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1339958"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1851)
                     /locus_tag="VD788_08475"
     CDS             complement(<1..1851)
                     /locus_tag="VD788_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76341.1"
                     /translation="MTSFRRIPRIERPYSYLVLPCAAVLAAVVSGSVIFPAETVPTDV
                     QIPGTQPAPLDNVPTINTVGNCNCHDFTGTPRPEDPPVFGWTGAMMANSGRDPLFWST
                     VAIAEQDFLPGGGGVGDLCLHCHSVKGWLEGRSTPTNGSGLDPTVDAEGVMCDFCHLL
                     TDPDMANSIPSAPAGQYVEEQHPPFSAYDEMNGAGYYGGAEYVVNSGGVRLGPYTDHD
                     ARHDVVPSPYLRDARYCGTCHDVSNPATGDLAHNHGAMIALTGGSSGVVNGPVAEKAA
                     MNNPPESYGVVERTFSEWSSSAYSQTEVDAVATLPSDVRVPGGAVDRAYHASLQGTCS
                     VGGEICKADTDCGAGEVCGGLSADYSDGTVRRFTCQTCHMSASLAQGAKQGAVRSDLP
                     GHDQTGGSYWIQDAIRWQDAHDTLVFGAGLTTTQDSAMAAAQARAASMLRSAAAIDAT
                     QVGNNLRVRVTNLTGHKLLSGYPEGRRMWIHVEWYDGAENEISEAENGAYGPLGVLVT
                     DLDGDSWNVQSILDPSSTKVYEAKPAMDKEWADQLLGLGYPTVMVLEWDRLTHAPRHT
                     LGDLAALPAGGMYHTFHFVLNNAVEEDNRIPPAGFRRDEAERRNALPVPDE"
     gene            complement(2140..2844)
                     /gene="uppS"
                     /locus_tag="VD788_08480"
     CDS             complement(2140..2844)
                     /gene="uppS"
                     /locus_tag="VD788_08480"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HXV76342.1"
                     /translation="MPAGIHVGIIMDGNGRWARARGLPRAAGHREGAKAVRRTIEAAP
                     DLGITTLTLYAFSSDNWRRPAAEVQTLMELLERYLRRESARCLEHGVRINVIGRRDRL
                     PPRVVRAVEDAERLTAPGRRLHLRLAIDYSGRHALTRAAAIAGELPAIDEPAFGALIN
                     RAIHSSPAAPDVDLLIRTSGEQRLSDFMLWEVAYAELFFTDVLWPAFGKRELKRAVRA
                     FHGRERRFGGLLERAV"
     gene            complement(2872..3264)
                     /locus_tag="VD788_08485"
     CDS             complement(2872..3264)
                     /locus_tag="VD788_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXV76343.1"
                     /translation="MADRQSASQRQSASARAQSKRLEALAGYLADRSAGEFDRLVYER
                     IRLGIMSALAVNRTMSFSDLKDLLRTSDGNLSVHARKLEDAGYIACSKSFEGRFPKTE
                     YRLTATGRAALERYLNHMEALIDATRGE"
     gene            complement(3257..3850)
                     /locus_tag="VD788_08490"
     CDS             complement(3257..3850)
                     /locus_tag="VD788_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76344.1"
                     /translation="MGVPAEPATLRRHAIDNLRYIRETMESASSFTAVPGWGGVAMGL
                     TALVAAAAASRATSVETWMSIWILDALAAFGIGGFTMARKARAAGIRVSRGAGRRFLL
                     GLSPPLLAALVLTVVFYRAGTAAMIPGTWLLLYGTGVMTAGTSSVRMVPIMGAGFVAL
                     GVAALFAPASWSNPLMAVGFGGLHVVFGAVIARRYGG"
     gene            complement(3922..5172)
                     /locus_tag="VD788_08495"
     CDS             complement(3922..5172)
                     /locus_tag="VD788_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXV76345.1"
                     /translation="MVKRLSPDETLEGLVRQLNTAARDAPQPLADSPAEPPARPRSPS
                     GRIEWSLAAPEPDGREWLERLLALARERFASDLLIVSDTPAALRIDGKLERAGGPISS
                     AGAGLLCSAIVPVERRATLAERGSVDFSFELDGLGRFRANVHRERGRWAAAVRLFPSD
                     PPDLEALQLPEQVGRFAELGYGLVLVTGPTGCGKTTTLAALVRRILERRRVHLISIED
                     PVEYDHGHGTSVVEHVEIGRDARSFADALRSVLRQDPDVLLVGEMRDRESVSIAITAA
                     ETGHLVLSTLHTGDGPQTIHRILDSYPAEQMAAVRAQLAASLAGVVSQQLLPRVDGRG
                     RVPAVEVMRMTHGIRNLIRRGKIEQLRSQMSLEPAAGMIDFDQSLARLVRRGLVAVEE
                     ARLRAREPAELDQWLGKPNSGKNL"
     gene            5319..6509
                     /locus_tag="VD788_08500"
     CDS             5319..6509
                     /locus_tag="VD788_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76346.1"
                     /translation="MGQYLGRAVVLAVIVFSSAVRAGGEIYGTVTTTRGDTFTGPIRW
                     DSQENFWSDTLDGRKPVSSAEPDADDGFRFSLFGWELINTKSNSRTYPEFSVPFGYLK
                     AVVPGGSGSVLLELKDGTRLTVEGPSTDFAGDLELAIDDIEEGRVVLPWRGIARVEFA
                     QGPGQGRDRERLYGTVKTETREFTGFVVWDRDESLLDDVLDGEERGREREIAMGEIAS
                     IEPDGASAAIVRTKAGAELRLSGTNDVNDDMRGVIVTLDGVGRVEVGWDELKRVDFRE
                     APTSPRYAAFDGGKRLRGTVHERDGRAYSGAITWDMDESYTWEFLDGESDRVVYAVPL
                     SHVRSIAPVGDRSAEVVLADGKILRLSGTNDVDERNKGVMITAADGSETILDWSAIER
                     VEFD"
     gene            6641..>6943
                     /locus_tag="VD788_08505"
     CDS             6641..>6943
                     /locus_tag="VD788_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76347.1"
                     /translation="MFVAELLVMLGLTAVASSAPGETHPFTVHDMLAMRRLSDPQRSP
                     DGDRIAFTLRTTDLDENRGRTDIWICGADGSGLRRLTTDPASDSNPRWAPDGKTIYF"
BASE COUNT         1119 a         2352 c         2422 g         1050 t
ORIGIN      
        1 ctcgtcgggc acgggcagcg cgttgcgccg ctccgcttcg tcacggcgga agcccgccgg
       61 cggaatgcga ttgtcctctt cgaccgcgtt gttcagcacg aagtggaagg tgtggtacat
      121 cccaccggcg ggaagcgctg ccaggtcgcc gagcgtgtgc cgcggggcgt gggtcagccg
      181 atcccactcg agcaccataa cggtcgggta gcccagaccg agcaactggt cggcccactc
      241 cttgtccatc gccggcttgg cctcgtagac cttcgtcgag ctcgggtcga ggatcgactg
      301 cacgttccag ctgtcgccgt cgagatcggt gaccagcaca ccgagcgggc cgtaggcacc
      361 gttctcggcc tcgctgatct cgttctccgc gccgtcgtac cattcgacgt ggatccacat
      421 ccggcggccc tcggggtatc ccgacagcag cttgtgaccg gtcaagttgg tcacccgtac
      481 gcggaggttg ttcccgacct gggtcgcatc gatcgccgcc gccgagcgca gcatcgatgc
      541 cgcgcgcgcc tgtgcggcgg ccatcgcgct gtcctgcgtc gtcgtcagcc ccgcgccgaa
      601 gaccagcgtg tcgtgcgcgt cctgccagcg aatggcgtcc tggatccaat agctgccgcc
      661 ggtctggtcg tgtcccggca gatcggagcg cacggcgccc tgcttggcgc cctgggccag
      721 cgacgccgac atgtggcagg tctggcaggt gaaccgccga accgtcccgt cggaatagtc
      781 ggccgacagg ccgccgcaca cctctcccgc gccgcagtcc gtgtcggcct tgcagatctc
      841 gccaccgacg ctgcacgtgc cctgcagcga ggcgtggtag gcccggtcga cggcgccacc
      901 cggcacgcgg acgtccgacg gcaacgtggc gaccgcgtcg acctcggtct gcgaataggc
      961 gctggacgac cactcgctga aggtgcgctc gacgacgccg tagctctcgg gcgggttgtt
     1021 catcgccgcc ttttcagcca ccggaccgtt gaccacgccg ctcgagccgc cggtgagcgc
     1081 gatcatcgct ccgtgattgt gtgccaggtc gccggtcgcc gggttgctca cgtcgtggca
     1141 ggttccgcag tagcgggcgt ccctcaggta cggcgagggg accacgtcgt ggcgcgcgtc
     1201 gtgatcggta taggggccga gccgcacgcc gccgctgttg acgacgtact ccgctccacc
     1261 gtagtagccc gcaccgttca tctcgtcgta tgccgagaac ggcggatgct gctcctcgac
     1321 gtactgtccc gccggggcgc tcggaatcga gttggccatg tcgggatcgg tcagcagatg
     1381 gcagaagtcg cacatcacgc cttcggcgtc gaccgtcggg tccaaccccg acccgttggt
     1441 cggagtggaa cgcccttcga gccagccctt cacgctgtgg cagtgcaggc acaggtcgcc
     1501 gacgccaccg ccgcccggca ggaagtcctg ctcggcgatc gccaccgtcg accagaacag
     1561 cgggtcgcga cccgagttcg ccatcatcgc tccggtccag ccgaagaccg gtgggtcttc
     1621 cggtcgcggc gttccggtga aatcgtggca gttgcagttc ccgacggtgt tgatcgtcgg
     1681 gacgttgtcg agtggcgcgg gctgggtgcc cgggatctgc acgtccgtcg gcaccgtctc
     1741 ggccgggaag atcaccgagc ccgagacgac ggcagcgaga acggccgcgc acggcaagac
     1801 cagataggag tagggtcgct cgattcgggg aatcctcctg aagctcgtca tgtggccccc
     1861 caagtgtcgt cacgtgcaag gaagcggcga ccaggcatcc cccgggtcac cggaggagag
     1921 caggttgcaa gggtcgttcc gcgtcatcgc gtcggcgggc gggccttcgc cgcggcaaat
     1981 cggcgagagc gcgacgtgtc ggcgatcggg aaacgactca gtcggcggac ctcgagcgtc
     2041 gaaaccccca gccgtgtgtg atcggtcgca gctcgggagt ttcgccgtcc agaaggtcgt
     2101 caatcacgcg agggaacggg cgagcggaag ctccgcgtct cagaccgccc gctcgagcag
     2161 accaccgaaa cgtcgttcgc ggccgtggaa ggcgcggacc gcccgcttga gctcgcgctt
     2221 gccgaatgcc ggccacagca cgtcggtgaa gaacagctcg gcgtaggcca cttcccacaa
     2281 catgaaatcg ctcagccgct gttcgccgct ggtccgtatc agcagatcga cgtcgggggc
     2341 cgcgggggag gagtgaatcg cgcgattgat caacgcgccg aaagccggct cgtcgatcgc
     2401 gggaagctca ccggcgatcg ccgccgccct cgtcaatgcg tgacgccccg agtagtcgat
     2461 cgccagccgc aggtgcagtc gccgccccgg ggcggtcaat cgctcggcat cctcgacggc
     2521 gcgaacgacg cgggggggca gccggtcgcg acggccgatc acgttgatcc gcaccccgtg
     2581 ctcgagacag cgcgcgctct cccgccgaag gtagcgctcg agcagctcca tcagcgtctg
     2641 gacctcggcc gccggacggc gccagttgtc cgacgaaaag gcgtagagcg tcagcgtcgt
     2701 gatccccaga tcgggggccg cctcgatcgt acggcgcacg gccttcgccc cttcgcgatg
     2761 gccggcggcg cgtggcagcc cacgggcccg cgcccagcgg ccgttgccgt ccatgatgat
     2821 cccgacgtgt attccagctg gcattccgaa ctcaccctcg cgtaaggcgc gtcattcgcc
     2881 ccgagtcgca tcgatcaggg cttccatgtg attgagatac cgttcgaggg cggcgcgacc
     2941 ggtggccgtc agccgatact cggtcttggg aaagcgaccc tcgaacgact tgctgcaggc
     3001 gatgtagccg gcatcctcca acttgcgcgc gtgcacgctc aagttgccgt cactcgttcg
     3061 caacaggtcc ttgagatcgg agaagctcat cgtccggttg acggcgaggg cactcatgat
     3121 gccgaggcgg atccgttcgt agaccagtcg gtcgaactcg cccgccgagc ggtcggccag
     3181 gtaaccggcg agggcctcga gccgcttgct ctgcgcacgc gccgaggccg attgacgctg
     3241 cgacgccgac tgtcgatcag ccaccgtacc gcctcgcgat caccgctccg aagacgacgt
     3301 gcagcccgcc gaaaccgacg gccatcagcg ggttggacca cgacgccggt gcaaacagcg
     3361 cggcgacgcc gagcgcgacg aaaccggcgc ccatgatcgg caccatccgg accgaggagg
     3421 tgccggcggt catcacgccg gtgccgtaca gcagcagcca ggtgccgggg atcatcgccg
     3481 ccgtccccgc gcggtagaac acgaccgtca gcacgagggc cgcgagcagc ggcggggata
     3541 agccgagcag gaatcgccgg ccggcgccac gcgagacgcg gatgcccgcg gcacgggcct
     3601 tgcgcgccat cgtaaagccg ccgataccga aggcggcgag ggcgtcgagg atccagatcg
     3661 acatccaggt ctcgaccgag gtggcacgcg acgcggctgc ggcggcgacg agcgcggtca
     3721 ggcccatcgc cacgccgccc caaccgggga cagccgtgaa cgaagaggcg gactccatgg
     3781 tttcgcggat gtagcgcagg ttgtcgatcg cgtgccgccg caaggtggca ggctcggcgg
     3841 ggacgcccac gggacctgga atcgccatgc gatcaaggta gagtcggatt tcacccgcgt
     3901 caagtacttg tcatcgcaaa gctataaatt tttgcccgaa ttcggcttgc cgagccactg
     3961 gtccaattcc gcgggttcgc gcgctcggag tcgggcctcc tcgacggcga ccaacccgcg
     4021 tcgcaccagc cgggcgagcg actggtcgaa atcgatcatc ccggcggcgg gctcgagcga
     4081 catttgcgag cgcaactgct cgatcttccc gcgacggatc aggttgcgta tgccgtgggt
     4141 catcctcatc acttcgaccg cgggcacgcg gccgcggccg tcgacccgcg ggagcaactg
     4201 ctgcgagacc acgccggcga gcgacgccgc gagctgcgcc cgaaccgccg ccatttgttc
     4261 ggccgggtag ctgtccagga tgcggtggat ggtctgcggg ccgtcaccgg tgtgaagcgt
     4321 cgacagcacg aggtggccgg tctccgccgc ggtgatcgcg atcgagaccg attcccgatc
     4381 ccgcatctcg ccgaccaaca acacgtccgg gtcctgacgc agcaccgaac gcagcgcgtc
     4441 ggcgaacgat cgcgcgtccc gcccgatctc gacgtgctcc acgacactcg tcccgtggcc
     4501 gtggtcgtac tcgaccgggt cctcgatgct gatcaggtgg acgcggcgcc gctcgagaat
     4561 gcggcggacc agcgcggcga gcgtcgtcgt cttgccgcag ccggtcggac cggtgacgag
     4621 caccagtccg taccccagct cggcgaaccg gccgacctgc tcggggagct gcagcgcctc
     4681 gaggtcggga ggatcggacg ggaacaaacg aaccgcagcg gcccaccggc cccgttcgcg
     4741 atggacgttg gcacggaagc ggccgagccc gtcgagctcg aacgagaagt cgaccgagcc
     4801 gcgctccgca agcgtggcgc ggcgctcgac gggaacgatt gccgagcaga gcaggcccgc
     4861 cccggccgac gagatcggac cgccggcacg ttcgagcttt ccgtcgatgc gcagcgccgc
     4921 gggcgtgtcg ctcacgatca acaggtcgct ggcgaagcgc tcgcgggcca gcgcgaggag
     4981 gcgctcgagc cattcgcgac cgtcgggttc gggggcggcg agcgaccatt cgatccgccc
     5041 ggagggcgag cggggacggg ccggaggctc ggcgggcgag tcggctagcg gctgcggggc
     5101 gtcgcgcgcc gcggtgttga gctgccggac cagcccctcg agtgtctcgt cggggctcaa
     5161 tcgcttgacc acgcgtcgtt cttacgccgt tcggcgaccg aaggcaagcc gtacgggcgg
     5221 acaagcgagg caccgcggtg gaaccccccc ggtcgttcag ccgttaatga aggtgagtgg
     5281 cgacaccgag cgcccgccgc ttcgaaggag ggggtccgat ggggcagtac ctgggccgtg
     5341 ctgtcgtcct cgccgtcatc gtcttctcgt cggcggtccg agccggcggc gagatctacg
     5401 gcacggtgac gacgacccga ggcgatacgt tcacgggacc gatccgctgg gacagtcagg
     5461 agaacttctg gagcgatacg ttggacgggc gcaagcccgt gtcgagcgcc gaacccgatg
     5521 cggacgacgg cttccgcttc agtctgttcg gctgggagct gatcaacacc aagagcaaca
     5581 gtcggaccta ccccgagttc tccgttccgt tcggctactt gaaagcggtc gtgcccggcg
     5641 gctcgggctc ggtcctgttg gagctgaagg acgggacgcg cctcaccgtg gagggcccgt
     5701 cgaccgactt cgccggcgat ctcgagctcg cgatcgacga catcgaggag ggccgggtcg
     5761 tgttgccctg gcgcggaatc gcacgggtcg agttcgctca ggggccgggc caagggcgcg
     5821 accgcgaacg cctctatggg acggtgaaga ccgaaaccag agagttcacc gggttcgtcg
     5881 tgtgggatcg cgacgagtcg ctgctcgatg acgtgctcga tggcgaggag cggggacggg
     5941 agcgcgagat cgcgatggga gagatcgcgt cgatcgagcc ggacggcgcg agcgccgcga
     6001 tcgtccgcac caaagccggc gcggagctcc gcctgagcgg cacgaacgac gtcaacgacg
     6061 acatgcgcgg cgtcatcgtc acgctggacg gcgtcggccg ggtcgaggtc gggtgggacg
     6121 agctgaagcg agtcgacttt cgcgaggcac ccacctcgcc gcgctacgcc gcgttcgacg
     6181 gcggaaagcg gctgcgcgga accgtgcacg agcgcgatgg ccgcgcctac tccggtgcga
     6241 tcacatggga catggacgag tcctacactt gggagtttct cgacggcgag tccgatcgcg
     6301 tcgtctacgc cgtcccgctg agccacgtgc gttcgatcgc cccggtcggg gaccgctcgg
     6361 ccgaggtcgt gctggcggac ggcaagatcc tcaggttgag cgggacgaac gatgtcgatg
     6421 agcggaacaa gggcgtgatg atcacggccg cggacgggtc ggagacgatc ctcgactggt
     6481 ctgccatcga gcgcgtcgag ttcgactgac cgatccccgg ctgccgccac ccatcgcacg
     6541 cacacgccgc ggtcctcgcc cgaggcgacg ccgcgcgtgg taggatgtcg cggttttctc
     6601 gtccgcgggc gggtcgcgcc cggcaggaga cgatatcgcg atgttcgtag cggagctgct
     6661 cgtcatgctg gggttgacgg ccgtcgcatc gagcgcgccg ggcgagacgc acccgttcac
     6721 ggtgcatgac atgctggcga tgagacggct gtccgacccg cagcgctcgc ccgacggcga
     6781 ccggatcgcg ttcaccctcc gtaccaccga cctcgacgag aaccgaggcc gcaccgacat
     6841 ctggatctgc ggcgcggacg gttcggggct gcgccggttg acgaccgacc ccgcgtccga
     6901 ttcgaacccg cggtgggcgc ccgacggcaa gacgatctat ttc
//
LOCUS       DASPGN010000293         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_521357, whole genome shotgun sequence.
ACCESSION   DASPGN010000293 DASPGN010000000
VERSION     DASPGN010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521357"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1123
                     /locus_tag="VD788_08510"
     CDS             92..1123
                     /locus_tag="VD788_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76348.1"
                     /translation="MLQSSFYWPLDIEFTPTIGIYIVDWNNHRIRRVTDQGRLETVVG
                     TNIIGDGPVDLSDTVFPGADGTECRLNHPTDVFERSNGTLAIVSWHNHKMREWDPLTG
                     KVFVSIGREPNCGGDGGSSLSAKLNQPVHATEGPDGSVYILDQRNQVVRKVDPSGNMS
                     TVVGTIVCPTPSPLDPGGFEGDGGDPVLAKMKQPTGPNPPPGGGIVVDAEGVLYFSDM
                     NNHRVRKVDFVANTITTVVGNGADAYAGDNAAPIDASLNLPADLEIGPDGRLYIADAG
                     NNVVRAVDFGANTIVTVAGSGTMGFGGDGGPATSAQLANPQGIAFDSVGDLYISDTFN
                     HRIRRVKMQ"
     gene            1134..2396
                     /locus_tag="VD788_08515"
     CDS             1134..2396
                     /locus_tag="VD788_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76349.1"
                     /translation="MRRSLRFQHRRGVVFLALLSALSLNCGSEETYAPPVDTCDEQYP
                     GTFSIDSPEVGHVYVWAGTGQPGAGAMGNLPGATRLYWPVEVNFDPAGSPIVLDWNNH
                     RVLALDGEGEFEKIIGRYFGNPNDGPALEADLNHPTHVVFSPDGSKLILSAWHNSILM
                     EMDLVTNWIAKYCGTGGRCFNGDGQARLASCLDLPVCALYHPISGELYFSDQANHIVR
                     RIDASGVVHVVAGSMPIFNGTSWVYQFGFGGDGGPATSALLNFERTQTANPSGKFCFA
                     ADGTIYIADTKNNAVRVVHTDGTIDTYAGKPAVGAGYSGDGGPATEAQLSDPRDVAID
                     TDGTLFIADTGNHVIRQVAPDGTISTAVGIARPRTADAISACALHDENGGLASEVHLT
                     APHGIELDADGNLWISDTLNQVIRIYYR"
     gene            2472..>3052
                     /locus_tag="VD788_08520"
     CDS             2472..>3052
                     /locus_tag="VD788_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXV76350.1"
                     /translation="MTVAAFNRVTPPLVERLRDALGTECVHVDDESLLLHASDETEDL
                     VFRPEVVVVPRTADAVAHVMRLATEYRVPVTPRGAGTGLSGGALPVHGGIVLSTKHLA
                     RILEIDRENLMAVVEPGVITQVFQEAVEAVGLFYPVDPASRGSCFLGGNLAECSGGPR
                     AVKYGVTKDFVTGVEAVLPDGTLVHHGGKLLKNV"
BASE COUNT          523 a         1078 c          965 g          486 t
ORIGIN      
        1 cgtcgcattg cccgtcgaga cgggacccgt cgggaccatt cgcacctggg ccggcaccgg
       61 agaagccgcc tacgacggcg attgcaaccc ggtgctgcag tcctcgttct attggcccct
      121 cgacatcgag ttcacgccca cgatcggcat ctacatcgtc gactggaaca accaccgcat
      181 ccgtcgcgtc accgaccagg gtcgtcttga aaccgtggtc ggcaccaaca tcatcggtga
      241 cggaccggtc gatctctccg acaccgtgtt tccgggcgcc gacggcaccg agtgccgact
      301 gaaccatccc accgacgtgt tcgagcgcag caacggaacc ctcgcgatcg tgtcgtggca
      361 caaccacaag atgcgcgagt gggatccgct caccgggaag gtgttcgtct ctatcgggcg
      421 ggagcccaac tgcggcggcg acggcgggag ctcgctgtcc gccaagctca accagcccgt
      481 gcacgcgacc gagggccccg atggatcggt gtacatcctc gaccagcgca atcaggtcgt
      541 ccgcaaagtg gatccgtcgg gcaacatgag caccgtggtc ggcaccatcg tgtgcccgac
      601 gccgtctccc ttggacccgg gcggcttcga aggcgacggc ggcgacccgg tactcgccaa
      661 gatgaaacaa ccgaccggcc ccaatccgcc gccgggtgga ggaatcgtcg tcgatgcaga
      721 aggcgttctg tacttctcgg acatgaacaa ccaccgcgtt cgcaaggtcg acttcgtcgc
      781 caacacgatc acgacggtgg tcggcaacgg agccgacgcc tacgccggcg acaacgccgc
      841 gcccatcgac gcatcgctca atcttcccgc cgacctcgag atcggccccg acggccggct
      901 ctatatcgcg gatgccggca acaacgtcgt acgcgcggtg gatttcgggg ccaacacgat
      961 cgtcacggtg gccgggagcg gaaccatggg atttggcggc gacggcggtc cggcaacgtc
     1021 cgcccagctc gcgaacccgc agggcatcgc gttcgattcc gtcggcgact tgtacatctc
     1081 ggataccttc aaccaccgca tccgtcgtgt gaagatgcag taggagaccc ggcttgcgac
     1141 gctcacttcg attccagcac cggcgcggtg tcgtgttcct cgcgctgctc tcggctctgt
     1201 cactgaactg cgggtccgaa gagacctacg ccccgcccgt cgatacctgc gacgagcagt
     1261 atcccggcac cttctcgatc gacagccccg aggtcggtca cgtctacgtg tgggccggca
     1321 ccggacaacc cggcgcgggg gcgatgggca atctgcccgg ggcgacgcgc ttgtactggc
     1381 cggtcgaggt caacttcgac ccggccggaa gccccatcgt gctcgactgg aacaatcacc
     1441 gcgtgctggc cctcgacggc gagggcgaat tcgagaagat catcgggcgc tacttcggca
     1501 atcccaacga cggccccgcg ctcgaggcgg acctcaatca tccgacccac gtcgtgttct
     1561 cgcccgacgg cagcaagctc atcttgagcg cgtggcacaa ctcgatcctc atggagatgg
     1621 acctggtgac caactggatc gcgaagtact gcgggaccgg cggacgctgc ttcaacggcg
     1681 acggccaggc ccgactcgcg tcgtgcctcg acctgcccgt gtgcgcgctc taccacccga
     1741 tctccggcga gctctatttc agcgaccagg ccaaccacat cgttcggcgc atcgacgcgt
     1801 cgggcgtggt ccacgtggtc gcgggctcga tgccgatctt caacggcacc tcgtgggtgt
     1861 atcagttcgg cttcggcggc gacggcggac cggccacgag cgcgctcttg aacttcgagc
     1921 gcacccagac ggcgaacccg agcgggaagt tctgctttgc cgcggacggc actatctaca
     1981 tcgcggacac caagaacaac gccgtgcgcg tcgtgcacac cgacggcacc atcgatacct
     2041 acgccggcaa gccggcggtg ggagcggggt actccggcga cggcggaccg gccacggagg
     2101 cacagctgtc ggatcctcgc gacgtggcca tcgacaccga cggcaccctc ttcatcgccg
     2161 acaccggcaa ccacgtgatt cgccaggtcg cgccggatgg gaccatcagc accgcggtcg
     2221 gcatcgcccg accccggacc gccgacgcca tctcggcctg cgcgctgcac gatgagaacg
     2281 gcggcttggc gagcgaggtt cacttgaccg ccccgcacgg gatcgagttg gacgccgacg
     2341 gaaacctctg gatatcggac acgctgaacc aggtgattcg catctactac cgctagcgcg
     2401 tcgcgcgctt cccgcgtctt cgtcgaaggg cgcgcttgcc ggtccggcgg gcgcgccctt
     2461 agtatctccc catgaccgtc gcggcgttca atcgcgtgac cccgccgctc gtcgagcgct
     2521 tgcgcgacgc gctggggacg gagtgcgtcc acgtcgacga cgagtcgctc ctcctgcacg
     2581 cctccgacga gaccgaagac ctcgtctttc gccccgaggt ggtcgtggtg ccgcgcaccg
     2641 cggacgccgt cgcgcacgtc atgcgcctcg cgaccgaata ccgcgtcccg gtcactccgc
     2701 gcggagccgg cacggggctc tccggtggcg cgctcccggt gcacggcggc atcgtgctat
     2761 cgaccaagca tctcgcgcgc atcctcgaga tcgaccgcga gaatttgatg gcggtggtcg
     2821 agcccggggt gatcacgcag gtcttccagg aagcggtcga agcggtgggg ctgttctacc
     2881 ccgtcgaccc cgcgagtcgc ggcagctgct tcctcggcgg caacctggcc gagtgctcgg
     2941 gcggtccgcg cgcggtcaag tacggcgtca ccaaggactt cgtcaccggg gtcgaagccg
     3001 ttctgcccga cggcacgctc gttcaccacg gcggcaagct gctgaagaac gt
//
LOCUS       DASPGN010000294         9843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1575313, whole genome shotgun sequence.
ACCESSION   DASPGN010000294 DASPGN010000000
VERSION     DASPGN010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9843
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575313"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VD788_08525"
     CDS             <1..765
                     /locus_tag="VD788_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV76351.1"
                     /translation="RTAALRGVDLAVARGEVVGVLGPNGSGKSTLVKVLGGGLGGYAG
                     SARIDGLEVGRTPRRRLARTVAVVEQEPEFSFAFTSLEIVLMGRHPHLAGLAFESERD
                     VELARAALARCGAAELAQRTIHELSSGERQRVVFARALAQEPRALLLDEPASFLDIRH
                     QVGLYDVVRALAERSGCAVLTVLHDLNLAAEYCDRIYLLRGGAVEADGPTRQVFTYAN
                     LKRVYETDVYVDTNALTGRMLVVPLPGRVTQRADDD"
     gene            804..1223
                     /locus_tag="VD788_08530"
     CDS             804..1223
                     /locus_tag="VD788_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HXV76352.1"
                     /translation="MARKLWLLAVGAVLLLPVCRAESARASRMWIVGWEAERASVVTG
                     EAAPVEFTLSIAREMPTPGWRLEVDAVEIDAGAHRIAVRVTERGPAGIVAQVITETPF
                     EIPLGPIEPGSYFVEIWARPSSEVAHQPAHALVVRAR"
     gene            complement(1232..2809)
                     /locus_tag="VD788_08535"
     CDS             complement(1232..2809)
                     /locus_tag="VD788_08535"
                     /inference="COORDINATES: protein motif:HMM:NF025499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HXV76353.1"
                     /translation="MTDAAKLTPADVEQIRERGVAIEEIERQIALLRAPRHFVRLERA
                     CSVGDGIVRIADEEARGLLEAHAAAAGQGRFCKFVPASGAATRMFRDLSVFRAAPRSA
                     EAFDEIRRSARAGDRPALALETFLAELERFPFADALRARMADDGAELADCAAAGRFRP
                     ILDALLGDGGLGYADLPKALLPFHRYPSETRTAFEEHLVEATGYVRDDGCVARLHFTV
                     SPEHRSAFDRLFRQVREPYRRRFDTEFEIGYSTQKPSTDTVAIERPGGALLRDETGRL
                     VFRPAGHGALIENLSELDADLVYIKNIDNVQPDRVKPLANRYKQMLGGFLVRLRRRTG
                     RVLEELRRGVGGLEDAARFAARRLHVDLNGRLDRSGSAAGRAWLIDRLRRPIRVCGVV
                     RNTGEPGGGPFWTSDVAGRRSLQLVEAAQVDPDDGGQRAIFAAATHFSPVDLVCALRD
                     ADGRPYDLGRFVDRDAVIVTTKTAKGRSLRALERPGLWNGAMAGWNTVFVEVPLETFT
                     PVKTVFDLLRPEHRAGE"
     gene            complement(2802..3479)
                     /locus_tag="VD788_08540"
     CDS             complement(2802..3479)
                     /locus_tag="VD788_08540"
                     /inference="COORDINATES: protein motif:HMM:NF022135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HXV76354.1"
                     /translation="MTGRTVRISVFTVLWLIAGRASSAGELWLGFEPTSPGPGDTVVV
                     HLYDGSAERPYLADRVGRFQRLWRRGRADLRGIDGALPAARFVGERAGVVLVVYDSAD
                     GLGFCKSLVVVGSPAPGDPIRWSEIGQRLEIVPQTDPVELRAAPGTLELQVLYEREPL
                     AGATVAAVRIAAPDDGLRATTDEIGVARLHLASGDWRITTTHRDPREPAPVSATLHLV
                     VGEAAGD"
     gene            3928..7056
                     /locus_tag="VD788_08545"
     CDS             3928..7056
                     /locus_tag="VD788_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXV76355.1"
                     /translation="MKILGHVDVRVLEQELLRVLVRDHPSTPGARSFVVVPTRRLVTH
                     LSKRLVRDGGAGAWLGVEFLTFHGLARSILVRAGARVPRVLAEPVRRSALRSVLAGLR
                     QNAWAAFVERRPGALGRLARAVDDLREAGVLPAELETCAGDHRGNRSLAEIYAAYHEA
                     LVALGVAGVTDGAGFIAAATPHAGEFAAASAQLLVYGAYEWLGVHLELIRELGRGREP
                     TVFVPVAPGAEVTRYAAAYLARHLPAETIVALDDPVGAERLPLRSLYDEHAEAEPVPP
                     GRIVLRHVQGATAECEGAAFLALDAVAAGTRPDEVAIVARSLTPYAAPLERAFDDRGL
                     NWTSSLTVPVRRHPIARDLLLSVRVVDEAFPRRAVVEWLRSPRLRFTSLPGVSHPPPR
                     DVAERYSRLAGIVSGFDEWTVVLPDWAARGAVSPSRRRRVARIAAAVRGLRDVLSGGA
                     ATWSRHAANLDRLIDLLQPAVPEARDEGAIAALRLLVRGMAELESLLGQREPVAFGEA
                     RRWLEDACSTTALATRERDDGGIRVLDAMQFRCLTHDRVLLIGTNSGVFPRLAREDPV
                     LGDPLRSELRERFDRPLTLAAEADDEEKLLLALILGAAGRRVDLSWQRADEGGRARTP
                     SLALRQVLRLTRGSPEMSGLACDGVVGVPSHPTQWLRFLAGELGLLAPEHAPLLDALT
                     GAGDDHFERLSRCYPELADGLTMLRATQDFAIRDPAFDARPRTARPFETISVSRFERL
                     GRCPLSFFFHDVLGVQELDPETTAATLPLNVIGNLVHDALERLYRRLAERGPFEADRA
                     DELRRAAREWLDEERGRLFGEVGDRLMPRLPGLWSITAEAWHDALAAFVDRDLERLAY
                     GGWVLDGLEQPIEGSIDLDDAPPVRVRGRLDRRLVRGDESIVGDYKTSGGLARRGEVL
                     WMLRGQTLQVPLYGMLAGGRARVELLGVGPAYDPLDAESARLTFSGLDPAALDGFRET
                     LRVLIGFARSGRFPLHSDRHCSWCCYRPACRRTDPPSLHRETLYDDGKRLAALLRKTL
                     RRPRLDPAEGDP"
     gene            7053..>9843
                     /locus_tag="VD788_08550"
     CDS             7053..>9843
                     /locus_tag="VD788_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXV76356.1"
                     /translation="MNRDAIVDRTERRIAATEFGANLVVVAGAGTGKTSLLVERVLNA
                     VGSARVGIDEIAAMTFTEKAAGEMRERLAEQLERLSSFARGHGVPDDGEAARSFDHLA
                     VALSLPRETIAERATVALERLERATVTTIHGFAADLLRGHPVAAGVDPDFVVDTGEKA
                     ERLRREVWERYLADELGREAPRRDLWKRLLARVTLADVERAAFAMADYDLPTSMLHPP
                     FELPTARALLGEEVRPIVDGLDALLARESGLAASARSYFTRARDHLSDFLVAEHRDWI
                     ARIDADAPFANRIRDGEPPSSFRRLTGFPREEAGRLARESRGFLRSLLAIDDDAAVWL
                     LELLAPFVIAFREAMLRQSLLTFDGLLVLARDLLRRVPAVRQQVKQRYRMLLVDEFQD
                     TDPVQYEIVLLLGERAGGAADDAFAVELEPGKLFVVGDEKQSIYRFRGADYTAYERAT
                     DRVIAGGGRRLELSANFRSVAGVVEPVNRLFGAGPDSRWRTSPYQPCYRAIHAVRPDE
                     SGAATELWTLRLDGAPPAETRRRAEGELIAEQIDRAVREGAARPREITILLRAFTSLA
                     PYLRPLRHRRIPFVVDGGRDFLERPEATQLIAAIRSLARPSDPVALLAFLRSPAGSVS
                     DEELSRYADHGGRWSFDERPDAARFPLISRRLRLLREMERETAELPADAAIRHVIERT
                     GMLPLNAAAFEGPQRVANLAKLAAVAGEMARDGQLSLAEVADSLESGRLAELESEAPL
                     ADDAIDAVRVSTIHRMKGLENRWVILPDLARFAPGSSQSVTAAAVRSPVGQTLALTVG
                     GKSSSGAVWLRREDRRHEIAEELRLLYVALTRARDRLVVVAGPTSREAPWLDALRPWG
                     FDPDAPPEDGARLCDGRVLHRVLRPVAEERPLEPHLHGSAEPACARYRLAVERLAAAR
                     GPTTPSGLVDEPAAER"
BASE COUNT         1325 a         3492 c         3560 g         1466 t
ORIGIN      
        1 cggaccgcgg cgctgcgcgg ggtcgacctg gccgtcgcgc gcggagaagt ggtcggtgtc
       61 ctcggcccga acgggtcggg caagagcacg ctggtcaagg tgctcggcgg tgggctgggc
      121 ggctacgccg gctcggctcg gatcgacggg ctcgaggtcg ggcgaacacc ccgccgtcga
      181 ctggcccgca ccgtcgcggt ggtcgagcag gagccggagt tcagcttcgc gttcacctcg
      241 ctcgagatcg tgttgatggg acgccacccg catctggccg ggctggcgtt cgagtccgag
      301 cgggacgtcg agctggcccg cgcggcgctc gcccgctgcg gcgccgccga gctggcccag
      361 cgcacgatcc acgaactgtc gtccggggaa cgccagcggg tcgtcttcgc ccgcgcgctg
      421 gcacaggagc cgcgtgcact gctgctcgac gagccggcga gcttcctcga cattcggcac
      481 caggtcggcc tgtacgacgt cgtgcgcgcg ttggccgagc gttccggctg cgccgtgctg
      541 accgtgctgc acgatctgaa tctcgccgcc gagtattgcg atcggatcta cctgctgcgc
      601 ggcggcgcgg tcgaggccga cgggccgacg cggcaggtgt tcacctacgc caacctcaag
      661 cgcgtgtacg agaccgacgt ctacgtcgac accaacgcgc tcaccggacg gatgttggtc
      721 gtgccgttgc cggggcgcgt cacgcagcgt gcggacgacg actgacgcgc tatcatgacg
      781 gtgtgagtct ccgaggtgaa cgaatggcac gcaaactgtg gctgctcgcc gtcggtgcgg
      841 tcctgctgct tcccgtctgt cgcgccgaat ccgcacgtgc gtcgcggatg tggatcgtcg
      901 gctgggaggc cgaacgcgcg agcgtcgtca ccggcgaggc ggcgccggtc gagttcacgc
      961 tgtcgatcgc acgcgagatg ccgacgccgg gctggcgtct cgaggtcgat gccgtcgaga
     1021 tcgacgccgg cgcgcatcgg atcgcggtcc gggtcaccga gcgggggccc gccggcatcg
     1081 tcgcgcaggt gatcaccgag accccgttcg agatcccgct cgggccgatc gaaccgggga
     1141 gctatttcgt cgagatctgg gcccgaccgt cgagcgaggt cgcgcaccag ccggcgcacg
     1201 ctctggtcgt ccgcgcccgg tagcagcccg ctcactcgcc ggcgcggtgc tccggacgca
     1261 gcaggtcgaa gaccgtcttg accggagtga acgtctcgag cgggacctcg acgaaaaccg
     1321 tgttccagcc ggccatcgcg ccgttccaca gacccggtcg ttcgagcgcg cggagcgacc
     1381 gacccttcgc agtcttggtc gtgacgatga cggcgtcccg atcgacgaag cgtccgaggt
     1441 cgtacggtcg gccgtcggcg tcccgcagcg cgcagaccag atcgaccggg ctgaagtggg
     1501 tcgccgcggc gaagatcgcc cgctggcccc cgtcgtcggg atcgacctga gccgcctcga
     1561 cgagctgcag cgaccgccgg cccgcgacgt ccgacgtcca aaacggcccg ccgcccggct
     1621 cgccggtatt ccgcacgact ccacaaacgc ggatcggcct ccgcaagcga tcgatcaacc
     1681 aggctcgccc cgccgcggac ccgctgcggt cgagccggcc gttcaggtcg acgtggagac
     1741 gccgggcggc gaaccgtgcg gcgtcctcga gcccccccac gccgcgtcgc agctcttcga
     1801 gcacgcggcc cgttcgccgt cgtaggcgga cgaggaagcc gcccagcatc tgcttgtagc
     1861 ggttcgcgag tggcttgacc cgatcgggtt ggacgttgtc gatgttcttg atgtagacca
     1921 gatcggcgtc gagttcggac agattctcga tcaaggcgcc gtggcccgcg ggacggaaga
     1981 cgagccggcc cgtttcgtcg cgcaacagcg ctccccccgg ccgctcgatc gcgaccgtgt
     2041 cggtggacgg cttctgtgtc gagtatccga tctcgaactc cgtatcgaag cggcggcgat
     2101 agggctcgcg cacctggcgg aacagccgat cgaacgcgga tcggtgctcc ggggagaccg
     2161 tgaagtgcaa ccgcgccacg cagccgtcgt cgcgcacgta gccggtcgcc tcgacgaggt
     2221 gctcctcgaa cgccgtgcgc gtttccgagg ggtagcggtg gaacgggagc agcgccttgg
     2281 ggagatccgc gtagcccaac ccgccgtcgc cgagcaaggc atcgagaatc gggcggaagc
     2341 gacccgccgc ggcacagtcg gcgagctcgg cgccgtcgtc cgccatccgc gcgcgtagcg
     2401 cgtcggcgaa ggggaagcgc tcgagctccg ccagaaaggt ctcgagggcc agcgcggggc
     2461 gatcccccgc gcgggccgag cgccggatct cgtcgaacgc ctccgccgag cgcggcgcgg
     2521 cgcggaagac cgacaggtcg cggaacatcc tcgtggccgc tcccgacgcg ggcacgaact
     2581 tgcagaatcg gccctgcccg gcggcggccg cgtgggcctc gagcagacca cgggcctcct
     2641 cgtcggcgat gcgaacgatg ccgtcgccga cggagcaggc gcgctcgagc cgcacgaaat
     2701 gacgcggcgc gcgcagcaag gcgatctgcc gctcgatctc ttcgatcgcc acgccgcgtt
     2761 cacggatctg ctcgacgtcg gccggtgtca gcttcgcggc gtcagtcacc cgccgcctcc
     2821 ccgacgacga ggtggagcgt ggccgacacc ggcgccggtt cccggggatc gcgatgcgtg
     2881 gtcgtgatcc gccagtcgcc cgacgcgagg tgtagccgcg cgacgccgat ctcgtcggtc
     2941 gtcgcgcgga gcccgtcgtc gggcgccgcg attctcaccg ccgcgacggt cgctccggcg
     3001 agcggctcgc gctcgtagag cacctgcagc tcgagcgttc ccggcgcggc ccgcagctcg
     3061 acggggtcgg tctgcgggac gatctcgagt cgctgtccga tctcggacca gcggatcgga
     3121 tccccggggg ccggcgatcc gacgacgacc agcgacttgc agaaaccgag gccgtcggcc
     3181 gaatcgtaga cgaccagcac gacgccggcc cgctccccga cgaatcgtgc agccggcagc
     3241 gctccgtcga taccccggag gtcggcgcgg ccccggcgcc acaaacgttg gaatcgaccg
     3301 acgcggtcgg cgaggtaggg gcgctccgcg cttccgtcgt agaggtggac gacgacggtg
     3361 tcgccgggac cgggcgaggt cggctcgaag ccgagccaga gctcgcccgc gctagacgcc
     3421 cggcccgcga tcagccacag gaccgtgaag accgagattc ggaccgttcg acccgtcacg
     3481 catcccctcc ggcggcgggg tcgagtgtac acgatcgctc gcgagggccg gcgtgacgga
     3541 gaagaccgct cgctgtcgcc aaatctccag gctttgcgcc gtacaacggg aaacgaaccc
     3601 cgcccgcgga tccgtgatct cgagggcgac cagcgtgacg gcggccggtg acacacggag
     3661 cggcaccgac gtggcaggcc ggccgcgacg cttgtcggcg tcgccggatg ccgcccggcg
     3721 cggagagatc gatggccata gctgcaggag gctactcatg acctaccgtg ttgctggaat
     3781 cccctacgga tcgctggcga tacgatacgc gattctgcgg caaacgcaac ctcgtttatg
     3841 cgctttgggg tcccgatgcc tcgggatgaa aaggaatctc tttggccggt tgaccgggtg
     3901 cgcattgcgg cggagactgg gcacccgttg aagatactgg gccacgtcga cgtccgagtg
     3961 ctcgagcagg agctgctccg ggtgctcgtc cgcgaccacc cgagcacccc cggcgcacgg
     4021 agcttcgtcg tcgtaccgac gcgacgcctg gtcacccacc tgtcgaagcg cctggtgcgc
     4081 gatggcgggg ccggcgcctg gctaggggtc gaattcctga cttttcatgg acttgcgcgg
     4141 tcgatcctcg ttcgggcggg agcgcgcgtc ccgcgggtgc tcgccgagcc ggtgcggcgg
     4201 agcgcgctcc ggtcggtgct ggccgggctg cgacagaacg cttgggcggc cttcgtcgag
     4261 cggcggccgg gcgcgctggg gcggctcgca cgagccgtcg acgatctgcg ggaggccggg
     4321 gtcctcccgg ccgagctcga gacctgcgcc ggcgatcacc gcgggaaccg cagcctcgcg
     4381 gagatttacg ccgcctacca cgaagcgctg gtcgccctcg gcgtggccgg tgtgaccgac
     4441 ggggccggct tcatcgccgc ggccaccccc cacgccggtg agttcgccgc cgcttccgcc
     4501 caactgctgg tctacggcgc ctacgagtgg ctcggtgtcc acctcgagct gattcgcgag
     4561 ctggggcggg gtcgcgaacc gaccgtcttc gtcccggtcg ccccgggtgc cgaggtcacg
     4621 cgttacgccg cggcctacct cgcccggcac ctaccggcgg agacgatcgt ggcgctcgac
     4681 gatcccgtcg gggcagagcg gctcccgctc cggtcgctct acgacgagca cgcggaggcg
     4741 gaaccggttc cacccggtcg gatcgtgctg cggcacgtcc agggagcgac cgccgagtgc
     4801 gagggcgccg cgttcctcgc cctcgacgcc gtcgcggcgg gaacccgccc ggacgaggtc
     4861 gcgatcgtgg cgcgcagctt gacgccctac gcggcgccgc tcgagcgggc cttcgacgac
     4921 cgcgggctga actggacgtc gtcgttgacc gtccccgtcc gccgccatcc gatcgcccgc
     4981 gacctgctgc tgtcggtccg cgtcgtcgac gaggcctttc ctcgacgcgc cgtcgtcgag
     5041 tggctgcgat cgccgcggct ccgtttcacg tcgctccccg gcgtgtccca ccccccgcca
     5101 cgcgacgtcg cggaacgata cagccggctc gccggcatcg tctcgggttt cgacgaatgg
     5161 acggtcgtgc tcccggactg ggccgcccgc ggggcggtct cgccatcgcg acgccgacgg
     5221 gtcgcgcgga tcgcggccgc cgtccgcggg ctgcgcgacg tgctgtccgg cggggcggca
     5281 acctggagcc gccacgcggc caacctcgat cgcctgatcg acctgctgca gccggcggtg
     5341 ccggaggccc gcgacgaggg cgcgatcgcg gcgctccggc ttctcgttcg tgggatggcg
     5401 gagctcgaga gcctgctcgg tcaacgcgag ccggtcgcct tcggcgaagc acgacgttgg
     5461 ctcgaggatg cttgcagcac gacggcgctt gccacgcggg agcgggacga cggcgggatc
     5521 cgggttctcg acgcgatgca gttccggtgc ctgacgcacg accgcgtctt gctgatcggg
     5581 acgaacagcg gcgtcttccc gcgcctcgcg cgcgaggatc cggtgctcgg tgatcccctg
     5641 cgctcggagc tgagagagcg cttcgatcgc ccgctgaccc tcgcggccga agccgacgac
     5701 gaggagaagc tgctgctggc gctgatcctc ggcgccgcgg gccgtcgtgt cgacctgtcg
     5761 tggcagcgcg cggacgaagg ggggcgcgcg cggacgccgt cgttggcgtt gcgccaggtg
     5821 ctgcggctga cgcggggctc gccggagatg agtggcctcg cctgcgacgg cgtggtcggc
     5881 gttccgtccc acccgaccca atggctccgc tttctcgccg gcgagctcgg cctgctcgcc
     5941 cccgagcacg ccccgttgct cgacgcgctg accggtgccg gcgacgatca cttcgagcgt
     6001 ctgagccggt gctaccccga gctcgccgac gggttgacga tgctgcgcgc cacacaagac
     6061 ttcgccatcc gcgacccggc cttcgacgcg cggccgcgaa ccgcccgtcc gttcgagacg
     6121 atctccgtca gccggttcga gcgactcggt cggtgtccgc tgagcttctt tttccacgac
     6181 gtgctgggcg tgcaggagct cgaccccgag acgacagccg cgacgcttcc gctgaacgtg
     6241 atcgggaatc tggttcacga cgcgctcgag cgcctgtacc gccgactcgc cgagcgcggt
     6301 ccgttcgagg cggaccgcgc ggacgaactg cgccgggcgg cgcgggaatg gctcgacgag
     6361 gagcgcggcc ggctgttcgg cgaggtcggt gaccgcttga tgccgcgtct gccgggtctg
     6421 tggtcgatca ccgccgaggc ctggcacgac gcgctcgccg cgttcgtcga ccgggatctc
     6481 gagcggctcg cgtacggagg ctgggtgctc gacgggctcg agcagccgat cgaggggtcg
     6541 atcgacctcg acgacgcgcc gccggttcgg gtccgcggcc gactggaccg tcggctggtt
     6601 cgcggtgacg agagcatcgt cggcgactac aagacctcgg gcggtctcgc ccggcgcggc
     6661 gaagtcctct ggatgctgcg cggacagacc ctgcaggtcc cgctctacgg catgttggcc
     6721 ggcgggcgag cccgcgtcga gttgctcggc gtcggtccgg cctacgatcc gctcgacgcc
     6781 gagagcgcgc gactcacctt ctccgggctc gacccggcgg cactcgacgg attccgcgag
     6841 acgctgcgcg tgctgatcgg cttcgctcgc agcggccgct tcccgttgca cagcgaccgc
     6901 cactgcagct ggtgctgtta ccgtccggca tgccgcagga ccgatccacc ttcgttgcac
     6961 cgggagacgc tctacgacga cggcaaacga ctggcggcgc tgctgcggaa gaccctgcga
     7021 cggccgcggc tcgacccggc cgagggtgac ccgtgaaccg cgacgcgatc gtcgatcgga
     7081 ccgaacggcg gatcgccgcc accgagttcg gcgccaatct cgtcgtcgtc gccggggcgg
     7141 gcaccggcaa gacgagcctg ctcgtcgagc gcgtgctcaa cgcggtcggc tcggcccggg
     7201 tcggcatcga cgagatcgcg gcgatgacct tcaccgagaa ggcggcgggc gagatgcgcg
     7261 aaaggctggc cgagcagctc gagcggttgt cgtccttcgc ccgcggccac ggcgtgcccg
     7321 atgacggaga ggccgcgcgg agctttgacc atctcgccgt tgcgctttcg ctcccgcgcg
     7381 agacgatcgc cgagcgagcg accgtcgcgc tcgagcgtct cgagcgagcg acggtgacga
     7441 cgatccacgg gttcgcggcc gacctgctcc gcggtcatcc ggtcgccgcc ggtgtcgatc
     7501 cggactttgt cgtcgatacc ggcgagaagg cggagcggct acggcgtgag gtatgggagc
     7561 ggtatctggc cgacgagctc ggccgggagg caccgcgccg cgatctgtgg aagcggctgc
     7621 tcgcccgggt gacgctcgcc gacgtggaac gggccgcttt cgcgatggcc gattacgacc
     7681 taccgacctc gatgctccat ccaccgttcg agctgccgac ggcgcgggcg ctcctcggcg
     7741 aagaagtgcg accgatcgtc gacgggctcg acgcgctgct cgcccgcgag agcgggctcg
     7801 ctgcctcggc gcgatcgtac ttcacgcggg cacgggatca cctgtccgat ttcctggtcg
     7861 ccgagcaccg cgactggatc gcgcgaatcg acgcggacgc gccgttcgcc aaccgcatcc
     7921 gcgacggcga gcctccgtcg agcttccggc ggctcaccgg tttcccgcgc gaggaggccg
     7981 gccgcctcgc gcgagaatcc cggggcttcc tccgctcgtt gctcgcgatc gacgacgacg
     8041 ccgccgtctg gctgctcgag ctgctcgcgc cgttcgtcat cgcgttccgc gaagcgatgt
     8101 tgcgtcagag cctgttgacc ttcgacggac tgctcgtgct ggcgcgcgac ctgctgcggc
     8161 gggtaccggc cgtccgccag caggtgaagc aacgctaccg gatgctgctc gtcgacgagt
     8221 ttcaggacac cgaccccgtg cagtacgaga tcgtgttgct gttgggcgag cgagccggtg
     8281 gcgctgcgga cgatgcgttc gccgtcgagc tcgagccggg caagctgttc gtcgtcggtg
     8341 acgagaagca atcgatctac cggttccgcg gagcggacta tacggcctac gagcgggcga
     8401 cggatcgcgt gatcgcgggc ggcgggcggc ggttggagct gtcggccaac ttccgcagcg
     8461 tggccggcgt cgtcgaaccg gtcaatcgcc tgttcggcgc cgggccggac agccgctggc
     8521 gaacgtcgcc ctaccagccc tgctatcgcg cgatccacgc ggtgcgcccc gacgagtcgg
     8581 gagcggcgac cgagctctgg acgttgcggc tcgacggcgc gccgccggcc gagacccgac
     8641 gccgcgccga gggtgagctg atcgccgagc agatcgaccg tgccgtgcgg gagggcgcgg
     8701 cccgcccgcg cgagatcacc atcctgcttc gcgccttcac ctcgctcgcg ccctaccttc
     8761 gacccctgcg ccaccggcgc atcccgttcg tcgtcgacgg aggtcgcgac ttcctcgagc
     8821 gcccggaggc gacgcagctg atcgccgcga tccgcagcct ggctcgtccg tccgaccccg
     8881 tcgccctgct cgccttcctg cggtctcccg ccggttcggt ctcggacgag gagctgtcgc
     8941 ggtacgccga ccacggcggg cgctggtcgt tcgacgagcg gccggacgcg gctcgctttc
     9001 ccctcatctc gcgacggctg cggctgttgc gcgagatgga acgcgagacg gcggagctgc
     9061 ccgccgacgc ggcgatccgc cacgtgatcg agcgcacggg gatgttgccg ctgaacgcgg
     9121 cggccttcga gggaccgcaa cgcgtggcca atctggccaa gctggccgcg gtggcgggcg
     9181 agatggcccg cgacgggcag ctgtcgctgg ccgaggtcgc cgactcgctc gaatcgggtc
     9241 gcctcgcgga gctcgagtcc gaggccccgt tggccgacga tgcgatcgat gcggtgcggg
     9301 tcagcacgat ccatcggatg aagggtctcg agaaccggtg ggtcatcctg ccggatctgg
     9361 cgcgctttgc tcccggttcg tctcagagcg tgacggccgc cgcggtccga tcgccggtcg
     9421 gccagacgtt ggctttgacg gtcggcggca agtcgagctc gggcgccgtt tggctgcgcc
     9481 gcgaggatcg acgtcacgag atcgccgagg agctgcgtct gctgtacgtc gcgctcactc
     9541 gcgcgcgcga ccggctggtc gtggtcgccg gaccgacgag ccgcgaagcg ccgtggctcg
     9601 atgccctccg cccttggggc ttcgacccgg acgcgccgcc cgaggatgga gcgcggttgt
     9661 gcgacgggcg cgtcctgcac cgggtgctcc ggccggtggc cgaggagcgg ccgctcgagc
     9721 cgcacctcca cggcagcgcc gagccggcct gcgcgcgcta ccgtctcgcc gtcgagcggc
     9781 tcgcggcggc gcgcggtccg acgacgccga gcggcctggt ggacgagccg gccgccgaac
     9841 gcc
//
LOCUS       DASPGN010000295        12778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_288731, whole genome shotgun sequence.
ACCESSION   DASPGN010000295 DASPGN010000000
VERSION     DASPGN010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12778
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288731"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4411)
                     /locus_tag="VD788_08555"
     CDS             complement(<1..4411)
                     /locus_tag="VD788_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV76357.1"
                     /translation="MRSRFSTGWRFTVVTAAVASLILLPFSQVLSSDRGTFIAGDQEG
                     GPGKVQKGEKAPGQVPTFSRANYTWSYASGPFYGDQSVGQKDRRAGFFHTPVGIFDLA
                     QGEPNFPGELKAKDARANAGPRYLVIQLDPETVKYGSFESIRDAIESNGGQIVRQLPD
                     STLIARVTPQGRSVIERSPDVYASILYPPAVKLHPSIGRIPLTDPAKAVSEVYELDVR
                     LFQGEDADAIAQTLTSLGGNVRAVAPDTVRVEIHRSKLAEVAGIEGVEMVFEHIAPLP
                     FSEETTTNVQTGKYNNGATPYHDVGVDGSGGGVGSPQILMVLDTGIQLDAADLSDTSM
                     SPGTPSGTHRKVISYSTTAAFPGGRGDSAGCDEGISGGFTHGHLVAAVALGNATDVSM
                     AYGDGFLATDPNGNSWKLDGVAPGAKLIAYDAQPTPAATSCGDPLLANIFAGDLYSGV
                     GTGSMGAAYTAGARTYNFSWGVEANLYDANAIDVDTFLFDNIEAMAFIAAGNAGLDAD
                     NDGVSDLGTLGAPATSKNALSIGASRAADDLAGAASAQEEERAAFSSAGPAPGNRVSP
                     QLLAPGDEEGGGTMGLSSEFGCRSSDNDQLDPVECDIFSQKEGTSFSSPAAAGAALLA
                     RDYFAQGFYPGGSQVPAAATNVSGALVKGLLIASAQFMIGDKPPTFTGHNPGGDNLTR
                     EYRFNNEQGYGRVKLDNVLPLDTWPASTTGLVVQDLVLSTGPRSLGGLDTVSASEVET
                     ATFDVCNPDEELRVVLTWLDPASLDGTLTNDLNLRLVSPMDDQGQQRIYFGNYFTDDN
                     NRNGVLDLNEDCPGLTGQIGTISQSAWSLPSCGNTVADTANPTEMIVLSPDPAGDGSI
                     PSQIEIGQWTIEVEGEAGLLSPQAYAVVVAGSVCQDSWVQLDAVEYSCNSTASATVYE
                     VPETGDLEPTTLTVESRTTIQVVSNGMVVDEEAVNMIRPDFDADKFVADGLVLTDGTA
                     RDPGNGALDVRNGDKLRVVYSDFGGDRISEASVNCTVKLGFGNIVFAQFGQDTTYFVS
                     GGCERNARGNFEFGFPDRYMDHDEAISFNFAFASNEAADLNNVDVALRCVYVDDSTNN
                     DSPEDCRPNSTDCTDPNRTNNETCDGEAGFGGTGTALMTILDSPKTISRISANSGLSA
                     NFSIQMASSIAGTPEIELILLVDAATSGKSSQAIAVSRHTVDVDENSVFYSTDFPTGG
                     VEQFDQSQFRAGDEILENPTTNLGTFQEDYRFETKTWSDLTAGGTKNLDLLSPWNFDV
                     NDGGFRSGILATTDESTITDTIAQWGEDKNFNNADDRRCTLDETQACTRNEDCMVGQG
                     TCASVEDRDPDNDAPEPDRNWSTQGGCGWQTRAPSTCASAPELGCFDDDDCPSADCTG
                     PEASTGGIWHTGTIAGTTGECLAVGASDGQCQAYETVGGTGGTLSWREHLITPIITKV
                     NGDDHKVEILDWRWSQAVELADSNSAWVWEFDTD"
     gene            4639..5490
                     /gene="larE"
                     /locus_tag="VD788_08560"
     CDS             4639..5490
                     /gene="larE"
                     /locus_tag="VD788_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HXV76358.1"
                     /translation="MQIWRRRIAAASGELARVDDLIVALSGGVDSAVLLAIARDALAG
                     RRLLAVTAVSASMPAVDARDAASVARYLAVDHRVVRTSELENPDYRANRGDRCFHCRD
                     VLFSSLGELATREGFGAVAYGAIADDLGDSRPGMRAALRHRILAPLLDAGFSKEDVRR
                     AADAEGLPVAEKPAAACLASRIPVGTEVNPARLRQVARAEEALRGLGFGQLRVRHHGE
                     IARIELDAAGFRRLVDHGLGQRVSEVVRAAGFRYVALDLDGYRPGSLNPVAIEPLPAT
                     PKRDGGQ"
     gene            complement(5415..5864)
                     /gene="sodX"
                     /locus_tag="VD788_08565"
     CDS             complement(5415..5864)
                     /gene="sodX"
                     /locus_tag="VD788_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-type superoxide dismutase maturation
                     protease"
                     /protein_id="HXV76359.1"
                     /translation="MIAAGRANLPLLACVLAVLATATWLATIWLGRPAVVAGRSMEPS
                     LRDGDRVIVDLWTYDRRPPRVGEVVLISAPETDGAWIVKRVARRGARGELWLLGDNPR
                     SSLDSRQWGWLPADRVVGRVVFRYWPPSRFGVAGRGSMATGFRLPGR"
     gene            complement(5861..6829)
                     /locus_tag="VD788_08570"
     CDS             complement(5861..6829)
                     /locus_tag="VD788_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV76360.1"
                     /translation="MVRRRTGWPPGLAAGLAILLALAAVGLAAPWLATDRAWIVRATD
                     GLHFPAWSGDPSVPIGVSNHDPAPLLRAPIPYGPNRVDLGVTLRPPSATHWLGTDDLG
                     RDVAARMVHGARVSMAVGLLAAALAVGVGVPLGAWAGYRRRWADFLVSRVVEAGLSFP
                     TLLLALAVLSFSRGWLAAVPDATRIAVVLGLTGWMPIARYVRAEVLKLTESDVVAAAR
                     AAGGNDVRIVARHILPSALAPVLVTAAFAVAAAIGTEAALSFLGLGVRPPEATWGVLL
                     AESREQVGHAWWLVVFPGIALFLAILACNLVGEGTRDLLDPRSTRR"
     gene            complement(6822..7760)
                     /locus_tag="VD788_08575"
     CDS             complement(6822..7760)
                     /locus_tag="VD788_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV76361.1"
                     /translation="MTLWFVTALVFVLIHLAPGSPLDGAEDRGGMTRLSDEDRAELAR
                     LYRLDQPLHRQYAAWVGGVLRGDLGRSFHDRRPVADKIVERIGVTAAINGLALGLMAA
                     LALPLGALSAWRPGSRVDRYGAWLTYAAYAIPVFWAALLFQLLFAVELRWLPLAGLDA
                     GGGSPTSPLVAAVDRLRHLVLPVVVLAVGGLAYLTRFVRAALLDSHAAENVVAARARG
                     LSALAVLVRHGFRRAAIPMLTLAGLLLPAVVGGSVIVENVFAIPGLGRMLVDAAFQRD
                     VPVLMALTLLSGVATLAGIVASDLAYLIVDPRIRRG"
     gene            complement(7802..9415)
                     /locus_tag="VD788_08580"
     CDS             complement(7802..9415)
                     /locus_tag="VD788_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV76362.1"
                     /translation="MSRRLTLLAAVFTLSLLGACSAPPQDEPPRREVRAVLGSDPASL
                     SLLGKMDTNTEILAAQITDSLVQYDAHLELVPRVAESWELSDDRRTLTFRLREGVRWH
                     DGRPVTADDVVFTVEKVRQPATENRTYAPGFDQLRSIEAPDARTVVARYVEIHPDYLD
                     GWRVPLIPRHLAGRDVDLLAGEFAARPVGCGPFRFVSYRVGQEIVLEANDDYWDGAPR
                     IAGLVFKIYPEQATAFQALLLGEIDVLRASPDQWQSARELRAGRPLDHLVYSKIGVWP
                     VVWNQNGSNPFFTDRRVRQAMMHALDRDAFIDKVVHGLARSGVTTFHPDTVWANPELA
                     PRPYDPALAGRLLDEAGWRDADGDGVREKDGRPFRFTLLVPKSPMELTRQIVVWQQDA
                     WSRIGVIAEIEELEFQAFRERRNAGRFEAASFNLAFTPGPDQFFDLFHSSAVDQGWNF
                     FGLADPEIDALLERGRATFDPAERQRLYSALQRLLYEQEPIGCTLMFSTPVLFDRHLR
                     GIEPTPLGIWLTTSGPRLWHWTEPVSTGG"
     gene            complement(9436..11517)
                     /locus_tag="VD788_08585"
     CDS             complement(9436..11517)
                     /locus_tag="VD788_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76363.1"
                     /translation="MNRSASLLCIAALSLASRAGETSSTVARTAEFAELKLGESDGVA
                     LSERGALFPAPRPVRLVADRLPGQPDQVWSMIAGPAGRLYLGTGPGGKVLEISPEGEA
                     SVFYSTGDLLVTALTFDRSGNLIVGTSPGGMLHRVAPDGSGSLWGETGERYVWSLALG
                     TDGRVYAGTGERGTVLRLGADGTAEPFFDSDEAHIVSLLAAPGGGLFAGGAGRGLLYE
                     IDDEGHGIVLFESELPQVSALALDADGRLVVALVAPPEREEPPPALELRLPDGVEVGR
                     TADGLGGLEEGSGPLLRGVIEGISEPAPSDGRRVRGQLVRLTDGVHGTEVWRSSDEAV
                     YSLLSTGDGVLFGTGEPARVYRLTADGDVALIATLAEAQATVLLESNRSAVLATSNPA
                     TAYRLEPRSSQPAVFVSHPIDAGASARWGAVRWEAAGTIEIYTRTGNSRDPDGTWSGW
                     SPALTDPERSRVVNPDGRYLQWRARFVPESTGDMRLRGVRVHYETYNRPPELKEFRPE
                     GGGHSFAGKAEFRCSVRDPDGDPVTVSLEIRPRGETDWRAAGGGEAAGDRPRPEWTEV
                     ELAWETAAIDEGRYEIRAIASDQTANAPDESGSVTGPTIGVVIDRSPPAYEIDARAGG
                     GVAITLRDALSEIRRLELVRDGRAAYRVRPQDGVCDSTTERFELQRPEAGAALRGFDA
                     AGNRVDIPLPP"
     gene            complement(11514..>12778)
                     /locus_tag="VD788_08590"
     CDS             complement(11514..>12778)
                     /locus_tag="VD788_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76364.1"
                     /translation="PLSVPLAVSGVGASGLAGVGALLSRAGFVPLQSGSYGSAAPAPP
                     PIEPGSAIGVKLVRGDLELTATGTVTWVDGGDLLAFGHPLFGLGSVDLPLTGATVETL
                     LPSVLQSSRFATPLGEIGALREDRVSGVLGRLGGTPRLIPVRFKLAHPLRGERDYSFD
                     IADDPLLSPLLLYVSLNGIIQSRERPFGNATVRLREGSVIKMAEGEDVELDNLFSGSN
                     ALDYGTGIPAYVLYLLMNNVWTQPRIAGVNLMLDYDDAPRTARIRRVSLDRYRVRAGE
                     TVETSVVLAPFRGPERVFGRSVLIPEETAPGRLTLHVGGALSVSRDEEQTEPVLPRDL
                     DQLVRLINQLRRNDRIYIAATTDDSGVVVDGVRLPNLPPSATSLMTRPKSRGSVVVVP
                     RRAILEEAIETGFAVEGSTRIELEIEAP"
BASE COUNT         2010 a         4354 c         4507 g         1907 t
ORIGIN      
        1 tgtcggtgtc gaactcccag acccaggcgg agttgctgtc cgccagctcg acggcctggc
       61 tccagcgcca atcgaggatc tcgaccttgt ggtcgtcgcc gttgaccttg gttatgatcg
      121 gcgtgatcag gtgctcgcgc caggacagcg tgccgccggt cccgccgacc gtctcgtacg
      181 cctgacactg accgtcgctg gcgccaaccg ccagacactc gccggtcgtg ccggcgatcg
      241 tgccggtgtg ccagataccg ccggtcgatg cctcggggcc ggtacagtcg gcgctcgggc
      301 agtcgtcgtc gtcgaagcaa ccgagctccg gggcgctcgc gcaagtgctg ggcgcacggg
      361 tctgccagcc gcagccaccc tgcgtgctcc agttcctgtc gggttccggg gcgtcgttgt
      421 ccggatcgcg gtcctcgacc gaggcgcagg taccctgccc gaccatgcag tcctcgttac
      481 gcgtgcaagc ttgagtctcg tcgagcgtgc agcgccggtc gtccgcattg ttgaagttct
      541 tgtcctcgcc ccattgagcg atggtgtcgg tgatcgtgct ctcgtcggtc gtcgccagga
      601 tgccggagcg gaagccaccg tcgttcacgt cgaagttcca cggcgacagc aggtccaaat
      661 tcttggttcc gcccgcagtg aggtcggacc aggtcttggt ttcgaaccgg tagtcttcct
      721 ggaaggtacc gaggttggtc gtcgggtttt cgaggatctc gtcacccgcg cggaactggc
      781 tctggtcgaa ttgctccacg ccacccgtcg ggaagtccgt cgagtagaac acggagtttt
      841 cgtcgacgtc gaccgtatgc cggctgacgg cgatggcctg cgacgacttg ccggacgtcg
      901 ccgcatcgac cagcagaatc agctcgatct cgggtgtgcc cgcgatcgac gacgccatct
      961 ggatcgagaa gttggccgac agacccgagt tggccgagat ccggctgatc gtcttcggcg
     1021 agtcgaggat cgtcatcaat gccgtgccgg tgccaccgaa gccagcctcg ccgtcgcagg
     1081 tctcgttgtt ggtccggttg ggatcggtgc agtcggtcga gttcggacgg cagtcttcgg
     1141 gactgtcgtt gttggtgctg tcgtccacgt agacgcagcg cagggcgacg tcgacgttgt
     1201 tgaggtcagc cgcctcgttc gacgcgaagg cgaagttgaa gctgatcgcc tcgtcgtgat
     1261 ccatgtagcg gtccgggaaa ccgaactcga agttgccgcg ggcgttccgc tcgcagccac
     1321 cggagacgaa gtaggtcgtg tcctgcccga actgcgcgaa cacgatgttg ccgaagccga
     1381 gcttgacggt gcagttgacg ctcgcctcgg aaatgcggtc tccgccgaag tctgaataga
     1441 ccacgcggag cttgtcgccg ttgcgtacgt cgagcgcacc gttgcccggg tcgcgcgccg
     1501 taccgtcggt cagcaccaac ccgtcggcga cgaatttgtc ggcgtcgaag tcgggacgga
     1561 tcatgttgac cgcctcttcg tcgacgacca tgccgttgct gaccacctga atggtggttc
     1621 gcgactcgac ggtcagggtg gtcggctcga gatcgccggt ctcgggcacc tcgtacaccg
     1681 tcgccgacgc cgtggagttg caggagtact cgaccgcgtc gagctgcacc cagctatcct
     1741 gacagaccga gcccgcaacc acgaccgcgt aggcctgcgg cgacagaagc ccggcttcgc
     1801 cttcgacttc gatcgtccac tggccgatct cgatctggct cgggatcgag ccgtctcccg
     1861 ccggatcggg cgagagcacg atcatctcgg tcgggttggc ggtgtccgcg acggtgttgc
     1921 cacaggacgg caggctccat gcgctctggc tgatcgtgcc gatctggccc gtcagaccgg
     1981 ggcagtcctc gttgaggtcg agcacgccgt tgcggttgtt gtcgtcggtg aagtagttgc
     2041 cgaagtagat ccgctgctga ccctggtcgt ccatcggcga gaccagtcgc agattgaggt
     2101 cgttcgtcag ggttccgtcg agactcgccg ggtcgagcca ggtgagcacg acacgaagct
     2161 cctcgtcggg gttgcagacg tcgaacgtcg ccgtctcgac ctcggaggcg ctgaccgtat
     2221 cgaggccacc gagtgagcgc ggcccggtcg aaagcaccag gtcctgaacg accagaccgg
     2281 tggtgctggc gggccaggtg tcgagcggga ggacgttgtc gagcttgacg cggccgtagc
     2341 cctgctcgtt gttgaaccgg tactcgcggg tcaggttgtc gccgcccggg ttgtggccgg
     2401 tgaacgtcgg aggcttgtca ccgatcatga actgggccga ggcgatcaac agccccttga
     2461 ccagcgcgcc cgagacgttc gtcgcggcgg cgggaacctg gctgccgccg ggatagaacc
     2521 cttgcgcgaa gtagtcccgg gcaaggagcg cggctcccgc ggcggccggg gacgagaagc
     2581 tggttccttc tttctggctg aagatgtcgc actcgaccgg gtccaactgg tcgttgtcgc
     2641 tcgagcgaca gccgaactcg gaggacagcc ccatcgtgcc gcccccttct tcgtcgccag
     2701 gagcgaggag ctgcggggag acgcggttgc cgggggccgg gccggcgctc gagaacgcgg
     2761 cgcgctcttc ttcctgcgcc gatgcggcac cggcgaggtc gtcggcggcg cgggacgcgc
     2821 cgatcgacag ggcgttcttc gaggtcgctg gcgcgccgag cgtgccgagg tccgacacgc
     2881 cgtcgttgtc ggcgtcgaga cccgcgttgc ccgcggcgat gaacgccatc gcctcgatat
     2941 tgtcgaacag gaaggtgtcg acgtcgatgg cgttggcgtc gtacaggttc gcttcgacgc
     3001 cccacgagaa gttgtaggtg cgcgcgccgg cggtgtaggc cgcgcccatc gagcccgtgc
     3061 ccacgccgga gtacaggtca ccggcgaaga tgttcgcgag caacgggtca ccgcacgagg
     3121 tggcggccgg tgtcggctga gcgtcgtagg cgatcagctt ggcgccgggc gcgacaccgt
     3181 cgagcttcca cgagtttccg ttggggtcgg tcgcgaggaa gccgtcaccg taggccatgg
     3241 agacgtcggt ggcgttgccc aacgcgacgg cggcaacgag gtgcccgtgg gtgaaaccac
     3301 ccgagatacc ctcgtcgcaa ccggcgctgt cgccgcggcc ccccgggaag gccgccgtcg
     3361 tcgagtacga gatgaccttg cggtgcgtcc cgctgggcgt tccgggcgac atgctcgtat
     3421 cggagaggtc ggcggcgtcg agctggatgc ccgtgtcgag gaccatcagg atctgcgggc
     3481 tgccgacgcc gccgccgcta ccgtcgacac ccacgtcgtg gtacggggtc gcgccgttgt
     3541 tgtatttccc ggtctggacg ttggtcgtcg tctcttccga gaacggaagg ggcgcgatgt
     3601 gctcgaacac catctcgacg ccctcgatgc ccgcgacctc ggcgagcttc gagcggtgga
     3661 tctcgacgcg aacggtgtcc ggggcgacgg ccctgacgtt accgccgagc gaggtgaggg
     3721 tctgcgcgat cgcgtcggcg tcctcgcctt ggaacaggcg gacgtcgagc tcgtaaacct
     3781 cgctcacggc cttggccgga tcggtcaacg ggatgcgccc gatgctcggg tgcagcttga
     3841 ccgccggcgg gtacaggatg ctcgcgtaca cgtcggggga ccgctcgatc acgctcctgc
     3901 cctgaggagt gacgcgcgcg atcagggtcg agtccggcag ctggcgaacg atctggccac
     3961 cgttgctctc gatggcgtca cggatcgact cgaacgagcc gtacttgacc gtttccggat
     4021 cgagctggat cacgaggtac cgcgggcccg cgttggcccg cgcgtctttc gctttcagct
     4081 cgccggggaa gttcggttca ccctgcgcca ggtcgaagat cccgaccggc gtgtggaaga
     4141 accccgccct ccggtctttc tggcctacgc tctggtcccc gtagaacgga ccgctggcgt
     4201 acgaccacgt gtagttcgcc cggctgaaag tcggaacctg gccgggtgcc ttctcccctt
     4261 tttgaacttt gcccggccca ccctcctggt cgccggcaat gaacgtgccg cgatccgaag
     4321 aaaggacctg cgaaaaggga agaaggatca agctcgcaac ggctgcggtc acgacggtga
     4381 accgccagcc cgtggagaaa cgactgcgca taccctcctc ctgcagtttt gatggcaagt
     4441 gaaacgggtg catgaatgga ccgtacactc tccccccctg agggatgcgc aacttgtacg
     4501 gtgataaaaa cgggttgtca aggaagaagc cggcgcggat cgcggtgaat tgcccgcaaa
     4561 acacggaaca ttattgatgc tagaatcgac ggcggcgtcg atttcccggt gtcggcgatt
     4621 gtagttaatt ggagctttat gcagatttgg cggcgtcgta tcgcggccgc gagcggcgag
     4681 ttggctcgcg tcgacgacct gatcgtcgcg ctttcgggcg gggtcgacag cgccgtgctg
     4741 ctcgcgatcg cacgggatgc gctcgcgggc cggcgcctgc tcgccgttac cgcggtctcg
     4801 gcctcgatgc cggcggtcga tgcgcgcgat gccgcaagcg tcgccagata tctggcggtc
     4861 gatcatcggg tcgtccgcac gagcgagctc gagaatcccg actaccgggc gaaccggggc
     4921 gaccggtgct ttcactgccg cgacgtgctg ttttccagcc tcggcgaatt ggcgacgcga
     4981 gagggtttcg gcgcggtcgc ctacggtgcg atcgcggacg atctcggtga ttctcgaccg
     5041 ggtatgcgtg ccgcgctgcg gcatcggatc ctcgcgccgc tgctcgatgc cggattctcg
     5101 aaagaagacg tccgccgcgc ggcggacgcc gaaggcctgc cggtcgcgga aaaaccggcc
     5161 gccgcctgcc tcgcatcgcg gattccggtc ggcaccgagg tcaatcccgc gcggctgcgg
     5221 caagtcgcgc gcgccgagga ggccctccgc ggtctcggct tcgggcaact ccgcgtccgc
     5281 catcacgggg agatcgcacg gatcgagctc gacgccgccg gcttccgacg attggtcgac
     5341 cacgggctgg gccagcgcgt gtcggaagtc gttcgcgccg ccggcttccg ctacgtcgcg
     5401 ctcgatctcg acggctaccg tcccggcagc ctgaacccgg tcgccatcga gcccctgccg
     5461 gcgacgccga agcgcgacgg cggccaataa cggaatacga cccgtccgac cacacggtcg
     5521 gcgggcaacc aaccccactg tcggctatcg aggctcgagc gtgggttgtc gccgaggagc
     5581 cacagctcac ctcgagcccc gcggcgcgcg acccgcttga cgatccacgc accgtccgtt
     5641 tcgggcgccg agatcaacac gacctcgccg acacgcggcg gacggcggtc gtaggtccag
     5701 aggtcgacga tcacgcgatc gccgtcgcgg agcgagggct ccatcgagcg gccggcgacg
     5761 accgccggac gtcccagcca gatcgtcgcg agccaggttg cggtggcgag cacggccaac
     5821 acgcaggcga gcaacggcag gttcgcacga ccggccgcga tcatcggcgg gtcgagcgcg
     5881 ggtcgagcag atccctcgtt ccctcgccga ccagattgca agcgaggatc gcgaggaaca
     5941 acgcgatacc cgggaagacg acgagccacc acgcatgtcc gacctgctca cgcgattccg
     6001 cgagcaggac gccccacgtc gcctcgggcg gtcgcacgcc cagtccgaga aacgacagcg
     6061 ccgcctcggt cccgatcgcc gcggccaccg cgaaggccgc cgtgacgagc acgggcgcca
     6121 gtgccgaggg caggatgtga cgggcgacga tcctcacgtc gttgccgccc gcggcacgcg
     6181 ccgcggcgac gacgtccgac tcggtcagct ttaggacctc ggcccgtacg taccgcgcga
     6241 tcggcatcca accggtgagc ccgagaacga ccgcgatccg ggtcgcgtcc ggcacggccg
     6301 cgagccagcc gcgcgagaac gacagcacgg ccaacgccag cagcagcgtc gggaagctga
     6361 gcccggcttc cacgacgcgc gagacgagaa agtcggccca gcggcgccgg tacccggccc
     6421 acgcgccgag cgggacgccg acgccgacgg ccaacgcggc ggcgagcagc ccgaccgcca
     6481 tcgagacgcg cgccccatgg accatccgcg cggcgacgtc gcggccgagg tcgtccgtgc
     6541 cgagccagtg cgtggcggag ggcggccgca gcgtcacgcc gagatcgacc cgattcggcc
     6601 cgtacgggat cggggcccgc agcagcggcg ccggatcgtg gttcgaaacg ccgatcggaa
     6661 cggaggggtc gccgctccac gccgggaaat gcaggccgtc cgtcgcgcgg acgatccacg
     6721 cccgatcggt cgcgagccac ggggcggcca ggccgacggc ggccagcgcg agcaagatcg
     6781 cgaggcccgc ggccaggccc gggggccagc cggtccgtcg cctaaccacg tcggatccgc
     6841 ggatcgacga tgaggtaggc caggtcggag gcgacgatcc cggccagcgt ggcgacgccc
     6901 gacagcagcg tcagcgccat cagcaccgga acgtcccgct ggaacgccgc gtcgacgagc
     6961 atccggccga gccccggaat ggcgaagacg ttctcgacga tcaccgagcc gccgacgacc
     7021 gccggcagca gcaggccggc gagcgtcagc atcggaatcg cggcgcggcg gaacccgtgg
     7081 cgcacgagca cggcgagggc cgacaggccg cgggcccgcg cggcgacgac gttctccgcc
     7141 gcgtgcgaat cgagcagcgc ggcgcggacg aagcgggtca agtaggccag accgccgacc
     7201 gccagcacga cgaccggcag gaccaggtgg cgcagtcggt cgaccgctgc cacgagcggc
     7261 gaggtcggcg agccgccgcc ggcgtcgagc cccgcgagcg gcagccagcg cagctcgacg
     7321 gcgaacagca gctggaagag cagcgccgcc cagaagaccg ggatcgcgta cgcggcgtac
     7381 gtgagccacg cgccgtaccg atcgacccgc gatccgggcc gccacgccga cagcgcgccg
     7441 agcgggagcg cgagcgctgc catcagaccg agcgccaggc cgttgatcgc ggccgtcacg
     7501 ccgatccgct cgacgatctt gtccgcgacc ggacggcggt cgtggaacga gcgtccgaga
     7561 tcgccgcgaa gcacgccgcc gacccaggcc gcgtattgcc gatggagcgg ctggtcgagc
     7621 cggtacaagc gcgcgagctc cgcgcgatct tcgtcgctca atcgcgtcat cccgccgcgg
     7681 tcctcggcgc cgtcgagcgg agagcccgga gccaggtgga tcaatacgaa cacgagcgcg
     7741 gtgacgaacc acaaggtcac gccgccggtc gcgatcctcc gccccgccgc gcgccacacg
     7801 gtcagccccc ggtcgacacc ggttccgtcc agtgccagag ccgtgggccg gaggtggtca
     7861 gccagatacc gagcggcgtc ggctcgatcc cgcggaggtg gcggtcgaac agcacgggcg
     7921 tgctgaacat cagcgtgcag ccgatcggct cctgctcgta cagcaggcgc tggagcgcgg
     7981 agtacagccg ctgtcgttcg gccggatcga acgtcgcccg gccgcgctcg agcagcgcgt
     8041 cgatctcggg atcggcgagg ccgaagaagt tccagccctg gtcgaccgcc gaggaatgga
     8101 acaggtcgaa gaactgatcg ggtcccggcg tgaacgccag gttgaacgag gcggcctcga
     8161 agcggccggc gttgcgccgc tcgcgaaagg cctggaactc gagctcctcg atctcggcga
     8221 tcacgccgat gcgggaccac gcgtcctgct gccagacgac gatctgccgc gtcaactcca
     8281 tcggcgactt cggcaccagc aacgtgaagc ggaacggccg cccgtccttc tcgcggacgc
     8341 cgtcgccgtc ggcgtcgcgc catccggcct cgtcgagcag gcggccggcc aacgcgggat
     8401 cgtatggccg cggcgcaagc tcggggttgg cccagaccgt gtcgggatgg aaggtcgtca
     8461 caccggaccg ggccaggccg tggaccacct tgtcgatgaa cgcgtcgcga tccaaggcgt
     8521 gcatcatcgc ctgacgcacc cggcggtcgg tgaagaacgg attggagccg ttttggttcc
     8581 agacgacggg ccagacgccg atcttggagt agacgaggtg atcgagcggt cgccccgccc
     8641 ggagctcgcg ggccgactgc cactggtcgg gcgacgctcg cagcacgtcg atctcgccga
     8701 gcagcagcgc ctggaacgcg gtcgcctgtt ccgggtagat cttgaacacg agcccagcga
     8761 ttcgcggagc gccgtcccag taatcgtcgt tcgcttcgag cacgatctct tggccgacgc
     8821 ggtagctgac gaagcggaac ggcccgcagc cgaccggacg ggcggcgaac tcgccggcga
     8881 gcagatcgac gtcgcgaccg gcgaggtgcc gcgggatgag cggtacgcgc caaccgtcga
     8941 gatagtccgg gtggatctcg acgtagcgcg cgacgaccgt gcgggcgtcc ggggcctcga
     9001 tcgagcggag ctggtcgaag ccgggggcgt aggtccgatt ctcggtcgcg ggctggcgga
     9061 ccttctcgac ggtgaagacc acgtcgtccg ccgtgaccgg tctgccgtcg tgccaacgca
     9121 cgccttcccg cagccggaag gtcagcgtgc ggcgatcgtc cgacagctcc cacgactcgg
     9181 cgacccgtgg caccagctcg agatgcgcgt cgtactgaac caggctgtcg gtgatctggg
     9241 cggccaagat ctcggtgttg gtgtccattt tcccgagcag cgagagcgag gccggatcgg
     9301 agccgaggac cgcacggacc tccctgcggg gcggctcgtc ctgcgggggc gcggagcacg
     9361 cgccgagcag ggacagcgtg aagaccgcgg ccagcagcgt gaggcgtcgc gacatgccgg
     9421 ctatcttgtc acggttcagg gtgggagggg aatgtcgact cggtttcccg cggcgtcgaa
     9481 gccgcgcaac gcggcgccgg cctcgggccg ctgaagctcg aagcgctcgg tcgtcgagtc
     9541 gcagacgccg tcttgcgggc gaacgcggta ggcggctcgc ccgtcgcgca ccagctcgag
     9601 ccgccggatc tcggacagtg cgtcgcgcaa cgtgatggcg acgccgccgc cggcccgcgc
     9661 gtcgatctcg taggcgggcg gcgatcggtc gatgacgacg ccgatcgtcg gcccggtgac
     9721 cgagcccgac tcatccggag cgttggccgt ctggtcgctt gcgatcgcac ggatctcgta
     9781 gcggccttcg tcgatcgcgg cggtctccca ggcgagttcg acctcggtcc actccggtcg
     9841 cggtcggtcg ccggccgctt ccccgccgcc ggcggctcgc cagtcggttt cgccccgcgg
     9901 gcggatctcg aggctgaccg tgaccgggtc gccgtccggg tcacggacgg agcagcggaa
     9961 ctcggccttg ccggcgaagg agtggccacc gccctcgggg cggaactcct tcagctccgg
    10021 tggccggttg taggtttcgt agtggacgcg gacgccgcgc agccgcatgt cgccggtcga
    10081 ctcggggacg aaccgcgcgc gccactgcag gtaccgcccg tcagggttca ccacgcggct
    10141 gcgctcgggg tcggtcagcg ccgggctcca gccggaccac gttccgtccg ggtcccggct
    10201 gttgccggtg cgcgtgtaga tctcgatcgt gccggccgcc tcccagcgga ctgcgcccca
    10261 gcgcgccgag gcaccggcat cgatcgggtg ggagacgaag accgcgggtt ggctcgaccg
    10321 aggctcgagc cgataggccg tcgcggggtt gctcgtcgcc agcacggccg agcgattcga
    10381 ttcgagcaac accgtcgcct gggcctcggc gagcgtggcg atcagcgcga cgtcgccgtc
    10441 cgcggtcagt cggtagacgc gggccggctc gccggtgccg aacagcacgc cgtcgcccgt
    10501 gctcaacagc gagtagacgg cctcgtcgct cgaccgccag acctcggttc cgtggacgcc
    10561 gtcggtcaat cgcaccagtt gcccgcgcac ccgccgcccg tccgacggcg cgggttcgct
    10621 gatgccctcg atcactcccc gcagcaacgg ccccgagcct tcctcgagac cgccgagccc
    10681 gtcggcggtg cggccgacct cgaccccgtc gggcaagcgc agctcgagcg ccggcggcgg
    10741 ctcctcgcgc tcgggcggag cgaccagcgc gacgaccagc cgtccgtccg catcgagtgc
    10801 gagcgcggag acttgcggaa gctcgctctc gaacaacacg atgccgtggc cttcgtcgtc
    10861 gatctcgtac aacagcccgc gacccgcacc gccggcgaac aacccaccgc ccggagcggc
    10921 gaggagactg acgatgtggg cctcgtcgct gtcgaagaag ggttcggccg tgccgtcggc
    10981 gccgaggcgg agaacggttc cccgctcgcc ggtgccggcg tagacacgcc cgtcggtgcc
    11041 gagcgccaac gaccagacgt aacgctcgcc ggtttctccc cacagcgagc ccgacccgtc
    11101 gggcgcgacg cgatgcagca tgccgccggg cgaagtgccg acgatcaggt tgcccgagcg
    11161 gtcgaaggtg agggccgtca ccaacaggtc tcccgtggag tagaagaccg acgcttcgcc
    11221 ctcgggcgag atctcgagca cctttccgcc gggaccggta ccgagataaa gccggcccgc
    11281 gggaccggcg atcatcgacc agacctggtc gggctgtccc ggcaggcgat cggcgacgag
    11341 acgaaccggt cgcggcgcgg gaaagagcgc gccgcgctcg gagagcgcca cgccgtcgga
    11401 ctcgccgagt ttcagctcgg cgaactcggc cgtgcgggcg acggtcgacg acgtctcgcc
    11461 ggcccgtgac gcgagcgaca gcgcggcgat gcacagcagc gacgccgagc ggttcatggc
    11521 gcctcgatct cgagctcgat ccgggtcgag ccctcgacgg cgaagccggt ttcgatcgcc
    11581 tcctcgagga tcgcccgccg cggcacgacg accacgctcc cgcggctctt ggggcgggtc
    11641 atcaggctcg tcgccgacgg tggcaggttc ggtaggcgaa cgccgtcgac gaccacgccc
    11701 gagtcgtcgg tggtcgccgc gatgtagatc cgatcgttgc gacggagctg gttgatcaac
    11761 cggacgagct ggtcgaggtc gcggggcagg acgggctccg tctgctcctc gtcacggctg
    11821 acggagagcg ccccgccgac gtgcagcgtc aatcgaccgg gcgccgtctc ctcggggatc
    11881 agcacgctgc ggccgaacac ccgctccggg ccgcggaacg gtgcgagcac gaccgatgtt
    11941 tcgaccgtct cgccggcccg cacccgatag cgatcgagcg agacgcggcg gatgcgcgcg
    12001 gttcgcggag cgtcgtcgta gtcgagcatc aggttgacac cggcgatccg cggctgcgtc
    12061 cagacgttgt tcatcaacag gtagagcacg taggccggaa tcccggttcc gtagtcgagg
    12121 gcattcgacc cggagaacag gttgtcgagc tcgacgtcct ctccctccgc catcttgatc
    12181 acggatcctt cgcgcagtcg cacggtggcg ttgccgaacg gccgctcccg gctctggatg
    12241 atcccgttga gcgagacgta cagcagcagt ggcgagagca gcgggtcgtc ggcgatgtcg
    12301 aacgaatagt cgcgttcgcc gcgcagcggg tgggccagct tgaagcgaac cgggatcagc
    12361 cgcggtgtgc cgccgagccg gccgaggaca ccgctgacgc gatcctcgcg cagcgcgccg
    12421 atctcgccga gcggcgtggc gaaccgcgag gactgcagca cgctgggcaa caacgtttcc
    12481 acggtcgcac cggtcagcgg caggtcgacc gatcccagtc cgaacagcgg gtgaccgaac
    12541 gcaagcaggt cgcccccgtc gacccaggtc acggtgcccg tcgcagtcag ctccaaatcg
    12601 ccgcggacca acttcacgcc gatcgccgag cccggctcga tcgggggcgg ggccggggcg
    12661 gcgctgccgt agctgcccga ctggagtgga acgaaccccg cgcgcgagag cagcgctccg
    12721 acgccggcca gaccggaggc gccgaccccc gaaaccgcga gcgggacgga cagcggga
//
LOCUS       DASPGN010000296         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_638936, whole genome shotgun sequence.
ACCESSION   DASPGN010000296 DASPGN010000000
VERSION     DASPGN010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638936"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VD788_08595"
     CDS             complement(<1..274)
                     /locus_tag="VD788_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76365.1"
                     /translation="MKQEIRFCTANDGVRLAYATAGRGAPLVKTANWLNHLEFDWESP
                     IWRHLLAALAARFSLLRYDERGNGLSDWDVDDISFDAMVRDLETVVD"
     gene            complement(335..>2279)
                     /locus_tag="VD788_08600"
     CDS             complement(335..>2279)
                     /locus_tag="VD788_08600"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXV76366.1"
                     /translation="PRARATATTDVRLSSFQARLLTLVVGSFAVVLTATLLLVSAANR
                     AGVRRQIDEALELATGVFHQSIADRVERLVEAARLLAGDFAFKSAYATGERATVRSAL
                     ENHRARIGADVMLLASLDYEILADTRDGPGVGSRLPVAQRLDELVAEGRLELSTIAFL
                     DDAPYQIVVVPLMVPIPDAWICVGFRIDDAFVERFKRLTLADVSLLARRPDGWSEIAS
                     TLAPARRAALPEALGRAETTDDIRALTLAGETFVSLATPLGEVDDAPLVAVLQRSLTS
                     AMAPHRRLRRVLIGLFFAALAVGLLFSLRLAASVSRPVLRLAAAAGRVERGDYRVSVV
                     PERPDEIGRLTESFNHMTRGLAERDHVRDLLGKVVSPAVAHELIHRGIELGGEERPVS
                     ILFADVRDFTTLCENVAPALILARLNRYLTAMSGVIETEAGVVDKYVGDAIMALFGAP
                     LPGDDHPLRAVRAALEMHRALSSLNAEFRAERAPEFHVGIGVNSGIVVAGNMGSPTRS
                     NYTVIGDGVNVASRLERLTKTYGVGLLVSRATRQAATGFEYRELDRVRVKGKREPVTI
                     YEPLGPPDRLDAATRERAATLGVALAAYRERNWDVAHREFTALRAAGDATLARLFLQR
                     IERLRGEPPGPDWDGTFARDAGG"
BASE COUNT          367 a          793 c          806 g          313 t
ORIGIN      
        1 agtcgaccac cgtttcgagg tcgcgcacca tcgcgtcgaa cgagatgtcg tcgacgtccc
       61 agtcggacag accgttgccg cgctcgtcgt agcgcagcag cgagaagcgc gcggcgagcg
      121 ccgcgagcag gtgacgccag atcgggctct cccagtcgaa ttcgaggtga ttcaaccagt
      181 tcgcggtctt gaccagcggt gcgccgcgac ccgccgtcgc gtaggccaga cgtacaccgt
      241 cgttcgcggt gcagaaccgg atctcctgct tcatcgcacg ctcattgtac ggccgacctc
      301 gggtcggaag gcgccgacga cccggcgggc ggcgctaccc gccggcgtcg cgagcgaacg
      361 tgccgtccca atcggggccg ggcggttcgc cgcgcagccg ttcgatgcgc tggaggaaca
      421 ggcgggcgag cgtcgcgtca cccgccgcgc gcaacgcggt gaactcgcgg tgcgcgacgt
      481 cccagttccg ctcgcgatag gccgcgagtg ccacgcccag cgtcgcggct cgttcgcgcg
      541 tcgcggcgtc gagccggtcg ggcgggccga gcggttcgta gatcgtgacc ggctcgcgct
      601 tgcccttgac ccgcacgcga tcgagctcgc gatattcgaa gccggtggcg gcttgccgcg
      661 tcgctcgact gaccagcaga cccacgccgt acgtcttggt cagccgctcg agccgcgagg
      721 cgacgttgac gccgtcaccg atcaccgtgt agttcgagcg cgtcggcgag cccatgttgc
      781 cggcgacgac gatgcccgag ttgacgccga tgccgacgtg gaactccggg gctcgttcgg
      841 cacgaaactc cgcgttcaac gacgacagcg ccctgtgcat ctcgagcgcc gcgcgcacgg
      901 cgcgcagtgg gtgatcgtcg ccgggaagcg gtgcaccgaa cagcgccatg atcgcatcac
      961 cgacgtactt gtcgaccacg cctgcttcgg tctcgatgac cccgctcatc gcggtcaggt
     1021 atcgattgag ccgcgcgagg atcagcgcag gtgcgacgtt ctcgcacagc gtggtgaagt
     1081 cgcgcacgtc ggcgaacaga atcgacaccg ggcgttcctc gcctcccagc tcgatgcccc
     1141 gatggatcag ctcgtgggcc accgcgggcg agacgacttt gcccagcaga tcgcgcacgt
     1201 ggtcgcgctc ggccaggccg cgcgtcatgt gattgaacga ctcggtcaac cgcccgatct
     1261 cgtccggccg ttcgggcacg acggagacgc gatagtcgcc gcgctcgacc cgtcccgccg
     1321 ccgcggcgag ccgcagcacc ggccgcgaga cggaggcggc gagccgcagc gagaacagca
     1381 agccgaccgc gagcgcggca aagaacagcc cgatcaacac gcgtcgcagg cgccggtgag
     1441 gcgccatcgc cgaggtcagc gaccgttgca gcacggcgac gagcggtgca tcgtcgacct
     1501 cgcccagcgg tgtggccaac gagacgaagg tctcgccggc gagggtcaat gcgcggatgt
     1561 cgtcggtcgt ttcggcgcgg ccgagcgctt cgggcagcgc ggcccgccgc gcgggggcga
     1621 gggtcgatgc gatctcgctc cacccgtcgg ggcgccgggc gagcaacgac acatcggcca
     1681 gcgtcagccg cttgaagcgc tcgacgaaag cgtcgtcgat gcgaaagccg acgcagatcc
     1741 aggcgtcggg gatcggcacc atcagcggga cgacgacgat ctgatacggc gcgtcgtcga
     1801 ggaacgcgat cgtcgaaagc tcgagccggc cctcggccac taactcgtcg agccgttggg
     1861 ccaccggcag ccggctaccg acgccgggcc cgtcgcgcgt gtcggcgagg atctcgtagt
     1921 cgagcgaggc gagcagcatg acgtcggcgc cgatccgcgc gcggtggttc tcgagcgccg
     1981 atcgcacggt cgcgcgctcg cccgtggcgt aggccgactt gaaggcgaag tcgccggcca
     2041 gcaggcgagc ggcctcgacg agccgttcca ctcggtcggc gatcgattga tggaagacgc
     2101 cggtggccag ctcgagcgcc tcgtcgatct gacggcgcac gcccgcgcgg ttggcggcgc
     2161 tgacgagcag gagcgtcgcc gtcagcacga ccgcgaacga cccgacgacc aacgtgagca
     2221 accgggcctg gaagctcgac aaccgcacgt cagtagtcgc ggtcgctcgc gcgcggggc
//
LOCUS       DASPGN010000297         9630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_873875, whole genome shotgun sequence.
ACCESSION   DASPGN010000297 DASPGN010000000
VERSION     DASPGN010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9630
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873875"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="VD788_08605"
     CDS             complement(<1..1044)
                     /locus_tag="VD788_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76367.1"
                     /translation="MVTGFNTDVEHRGVVFHVQTEEKGPSNPVMVTLVYCQGEVIASY
                     EASYSSQSGTNALSDEQIRTRMRRQHRGLIEAIRGGKYDPSTRYPGEDRRASPSLDEL
                     VLGYLENEIDRARVVASGRNERQVAELRGAPDPVKVRRLLGRLERFLDAARRAEATAA
                     RVEPPTPLPAVTTTYRRPPPPRVAVVRPPVIETVVPVRGEPGGRHRTPRSTWIVTAAV
                     LACGLASLFFFGRPPAMPARPTFVAAPFAPPPSRPLPEPARPVLDPRPGPESLPATGE
                     TRVEEVEALRPAERSRPDPGGSETVPPASGLAEPHRPRPAPASEPPAVAVERPAPTPI
                     DPREPILPPVDAEQ"
     gene            1222..3447
                     /locus_tag="VD788_08610"
     CDS             1222..3447
                     /locus_tag="VD788_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXV76368.1"
                     /translation="MTKKGTQPFVPLVLCCVALLAAGCGPGGPPPDILLVTLDATRAD
                     RLGCYGHAPAATPAIDALAGAGVRFDRAYAQAPLTLPSHVSLLTGTYPAVHGVRVDAQ
                     GRVPEGVETLAERLAEAGWETAAFVSSFDLGAAFGLDRGFDLFDSISGDGSQPTRIAA
                     DRPADATTDAALAWLEARGDRPFFAWLHYNDAHWPYNPPAELTADGADPYDGEIAFVD
                     RQLARVVAWLESTGRLERTLIVVAADHGESLDDHGEPRHGLFVYDATVRVPLIVRWPA
                     RLPAGLVVDEPVALIDVLPTILDLTGLGIGEQVDGRSLRPAFDGKLEAADVVGESHLA
                     EIGYGWAPLAFLVRGGKKFIDAPVPELYDLEDDPGELRNLAPALPGETVAMREALFRI
                     ESRMTPLAPHAAAADPETRARLESLGYLGGTSPDAPAGDAPDPKTMIALLRGHFYGLG
                     LNRAGRYQESVAVLEQLVAESPRSCDPYADLGWSYLATGRSRSAEGAYRESLRALPQH
                     GERLWGLAEALRRQGRYEDAIEAYRASLVQRPEMFESHLGLALSYGSLQRFDEAFEHT
                     RRQVEINPSSTIALTNLTSLGVQVGEFEAAISASERLVELDPTSLELHFARWNAMTRS
                     GKPEREIAALRDSHRRFPDQWMFIWALAWRLAVTRDTAEDDLREAVRLGEQAVELNPN
                     HARSWDAFAAAQAAVGDYEQAVAAARQAHALAAASPDTSLAGQIAARLESYETGRPYY
                     E"
     gene            complement(3463..4095)
                     /locus_tag="VD788_08615"
     CDS             complement(3463..4095)
                     /locus_tag="VD788_08615"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HXV76369.1"
                     /translation="MRWAAALCAIAGLWAIAGCGSVPPTHYYVLETPRPVGPGGGDTG
                     ARAHVVGVTDFRVDPPYDRDTLVYRVAAHPTEVGFYDYHRWAAPLERMLPVVFARGLS
                     LADGATAFEPAVPGRAYAALLHGRVTTIEEIDHADREEVRLRVELVLALPDGSQLWSY
                     AAAGGRALDADEVVEIAEAMNATLAELVQEARPAVAAALGEHRQRIGAGH"
     gene            complement(4092..5072)
                     /locus_tag="VD788_08620"
     CDS             complement(4092..5072)
                     /locus_tag="VD788_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXV76370.1"
                     /translation="MARDERRADIRVGLFVIGALGLFVSGLFWIAGTGAWGDGRVVYL
                     VSLRDSGGVQAGDRVRVAGVPAGKIRRVELRPGDERPVRFDVSLRADVPLHTDAVARI
                     TSAGLLGAAFLEIDPGTARLPVLPAGAEIRGQETIGMDDAMARIDDLGERVAKLLDQT
                     SSLLDAIAVDLDQALEGANRLMSGQNVDNVTALLSKLNRAVDEFGPQLGSLVSRLDSV
                     AAKADRELDALPDLARRLDGLAGDLRTAIGPDGERLRSLLERADASLGSADDALSVLG
                     DNRHELGDALRDLRDTAANLKAFSQTIKERPYSLVRMSAPPQRAPGQGAR"
     gene            complement(5085..5882)
                     /locus_tag="VD788_08625"
     CDS             complement(5085..5882)
                     /locus_tag="VD788_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV76371.1"
                     /translation="MDTADVVIRVSGLSKRFGGNPVLQGIDLEVRHGETLVILGGSGS
                     GKSTLLRCLVGLEQPDAGRVTIQGVDLFDSDPVTLSRLRERIGMAFQGGALFGSRTVA
                     ENVELPLEEFTAMPRLTRRIVARIKLGMVGLEDAMDLYPAELSGGMVKRAALARALAL
                     DPDVLLFDEPSAGLDPVTAAELDRLMIQLKRAFGVTTVVVTHELHSAFAIADRIALID
                     RGRFRIVDTPSVVRACDDPLVRRFLDREPAPSEDSGKKFRRFLDDLR"
     gene            complement(5887..6684)
                     /locus_tag="VD788_08630"
     CDS             complement(5887..6684)
                     /locus_tag="VD788_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV76372.1"
                     /translation="MLGGLIRALGRAANNLFAEAGAMSRLALDSSRRLVLNPFGRGKV
                     RSRETLEQLARAGNGSLPLVSLICLLVGMIMALQSAYQLRRFEAVDLVASLVAVSMSR
                     ELAPLLTAIIVAGRFGSAIAAELGTMRVSSEIDALEAMGIDPTSYLVVPRLVGLSVAM
                     PCLTVFADAVGILGGMLVGVGALGLGSGRYLEDTVDALVLEDVLTGLVKSLAFACIIG
                     LVGCREGLATRGGAEQVGRATTRAVVRSIVLVICADLIVTAWFYVRG"
     gene            complement(6694..7062)
                     /locus_tag="VD788_08635"
     CDS             complement(6694..7062)
                     /locus_tag="VD788_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXV76373.1"
                     /translation="MQARTFETEIAAIRGRDDASTLRVHGRVRYPEAPELRRRLLRWI
                     DETPARRLVVSLGEVADIDTSGAAVLVEALLRGRERGKPVLLCSPSPSVMRMFRLAGF
                     EEALDACCPTPAETARRLMT"
     gene            7215..7784
                     /locus_tag="VD788_08640"
     CDS             7215..7784
                     /locus_tag="VD788_08640"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV76374.1"
                     /translation="MTDVARLLERCRRGDDLAWETLVRRYQSRVYAVAYHYVREADEA
                     RDVAQEIFVRVYQKLDSFDHSSNFLAWLMRLARNACIDRLRRRAARPPASDLPIGESI
                     EVESTEPDPEEHARRESERRLLHRALAGMSEANREMILLREIQGLKLEEIASMLGLPL
                     GTVKSRSSRARIELASRVRAIVPAFGASS"
     gene            7781..8647
                     /locus_tag="VD788_08645"
     CDS             7781..8647
                     /locus_tag="VD788_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV76375.1"
                     /translation="MSRFDCDAFERQLEALLDGRSSAAARARAVRHLRVCGDCAELMR
                     LATMPPPGPVDLVDGVMARTAGSACAPVRRRLCALVDGELDPLQAEQARSHVDSCADC
                     ATLLAVLGALPAALVLLREMALDDRFTADVLARIRSLPSKAPGRTPSWLRRAVQLLER
                     PRFPLEAAYVMTTLVVLFAGIPSAPIAGVSRRAFEIASADLPAGIEQSVVGVGDRVGG
                     SAKQTWDSASEQVSRDARRRADAVAELSSVLASDIRTGIGTLWERIASVEGEQDDRLS
                     PAENDGRTGDGR"
     gene            8644..9165
                     /locus_tag="VD788_08650"
     CDS             8644..9165
                     /locus_tag="VD788_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76376.1"
                     /translation="MNTQADHNPRPSLEDPGRKSVVLAVVLSALPGLGQVYVGYYPRG
                     FTHSLVAASLIAVLSSLTEFSPLFPLGMIFLFFFWFYNMIDAGRRAAMYNHVLAGGEI
                     VDLPKDISIPGLRGSIVGGLVIIAVGAIVLSHTAYDVPLDWLEDWWPAGVVLFGVYLV
                     VKGVQERVGTSLG"
     gene            complement(9249..>9630)
                     /locus_tag="VD788_08655"
     CDS             complement(9249..>9630)
                     /locus_tag="VD788_08655"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXV76377.1"
                     /translation="VHIKGTLSGREDLTVDGRVEGKITLKEHVLTIGANGQIDAELQA
                     KAVIVHGKVTGNITADNKVEIANSGTVMGDIRAPRVSISDGAMFKGSIDMDVASSARR
                     QEQGKREDVAVTKELQHQTAKAAS"
BASE COUNT         1439 a         3456 c         3316 g         1419 t
ORIGIN      
        1 ttgctcggca tcgaccgggg gcaggatcgg ctcgcgcgga tcgatcggcg tcggggccgg
       61 tcgctcgacc gctaccgccg gcggctcgga tgccggcgcc ggacggggtc ggtgcggttc
      121 ggcgagcccg ctcgccggag gaaccgtctc gctccctccg ggatcgggcc gcgaccgctc
      181 ggccggccgc agcgcctcga cctcttcgac ccgggtttcc cccgtcgccg gcaacgactc
      241 gggccccggt cgggggtcga gcaccggtcg cgccggctcg ggcagcggtc gagacggggg
      301 aggggcgaac ggtgccgcca cgaaggtcgg ccgtgccggc atcgccggcg gtcgtccgaa
      361 gaagaacagg ctcgccagac cacaggccag caccgccgcg gtcacgatcc aggtcgagcg
      421 aggggtgcga tgtcggccgc ccggctcgcc ccgcacgggg acgaccgtct cgatgacggg
      481 aggtcgcacc acggcgaccc gcgggggagg cggacggcgg taggtcgtcg tcacggcagg
      541 gagcggcgtc ggcggctcga cccgcgccgc cgtcgcctcg gcgcggcgtg ccgcgtcgag
      601 gaaacgctcg agccgaccga gcaaccggcg caccttgacc ggatcgggag cgccgcgcag
      661 ctcggcgacc tgccgctcgt tccgcccgct cgccacgact cgtgcccggt cgatctcgtt
      721 ctcgagatag cccagcacca actcgtccag gctcggcgag gcccgccgat cctcgccggg
      781 gtatcgcgtc gacgggtcgt actttccgcc ccggatcgct tcgatcaacc cgcgatgctg
      841 gcgtcgcatg cgtgtgcgga tctgctcgtc ggatagtgcg ttcgtgccgg attgcgagga
      901 gtaggaggcc tcgtacgagg cgatcacctc tccctggcag tagacgaggg tgaccatcac
      961 cgggttcgaa ggaccctttt cctcggtctg gacgtggaac acgacgccgc gatgctcgac
     1021 gtccgtgttg aaaccggtca ccatgcacgc acccgtagcc gacgcggggg cctcccgtcc
     1081 ggactacctc gaagtcaaag atgcgcctgg ccgggtacgg gtcaagaacc gggcgagcgc
     1141 ggcccacccg gcgagtccgt gggttgcgac ggatcgttac gaagggtatc tatgacagcc
     1201 ctggagacga cggccgtcgc gatgacaaaa aagggcactc aaccgttcgt tccgctcgta
     1261 ctctgttgcg tggcgttgct ggccgccggc tgcggacccg gcgggccacc cccggacatc
     1321 ctgttggtga cgctcgacgc gacccgcgcc gaccggctcg gctgctacgg ccacgcgccg
     1381 gccgccaccc cggcgatcga cgcgctggcc ggtgccgggg tccgtttcga tcgcgcctac
     1441 gctcaggcgc cgttgacgct gccgtcccac gtctcgctgc tgaccgggac ttacccggcc
     1501 gtccacggcg tgcgggtcga cgcccagggg cgagtgcccg agggagtcga gacgttggcc
     1561 gagcggctcg ccgaggcggg ttgggagacg gccgccttcg tctcgagctt cgacctgggc
     1621 gccgccttcg gtctcgaccg cggcttcgac ctgttcgact cgatctcggg cgacggctcg
     1681 cagccgacgc ggatcgccgc cgaccggccc gccgacgcca cgaccgacgc ggcgctggcc
     1741 tggctcgaag cgcggggcga ccgcccgttc ttcgcctggc tgcactacaa cgacgcgcat
     1801 tggccgtaca acccgccggc cgagctcacc gccgacgggg ccgacccgta cgacggggag
     1861 atcgcctttg tcgaccgtca gctggcccgc gtcgtggcgt ggctcgaatc caccggacgg
     1921 ctcgagcgca cgctgatcgt ggtggcggcc gatcacgggg agtcgttgga cgaccacggc
     1981 gagccccgcc atgggctgtt cgtctacgat gcgacggtgc gcgtgccgct gatcgtccgc
     2041 tggcctgcgc gcctgcctgc gggcctcgtc gtcgacgaac cggtcgcgtt gatcgacgtg
     2101 ttgccgacga tcctcgatct gacggggttg gggatcggcg agcaggtcga tggtcgcagt
     2161 cttcgaccgg cgttcgacgg taagctcgaa gcggccgacg tcgtcggcga gagccacctc
     2221 gccgagatcg gctacggctg ggccccgctc gcctttctcg ttcgcggcgg caagaagttc
     2281 atcgacgcgc cggtgccaga gctgtacgac ctcgaagacg atccgggcga gctgcgcaac
     2341 ctggcgccgg cgcttcccgg cgagacggtc gcgatgcgcg aggcgctgtt ccgcatcgag
     2401 tcccggatga cgccgctcgc gccgcacgcc gccgccgccg acccggaaac gcgggcccga
     2461 ctggagtcgc tcggctacct gggcggcacg tcgccggacg cgccggcagg cgacgctccg
     2521 gacccgaaga cgatgatcgc cctgctgcgc ggccacttct acggtctcgg cctcaaccgc
     2581 gccggccgct accaggagag cgtcgcagtg ctcgaacaac tggtcgccga gagcccacgg
     2641 tcgtgcgacc cctacgcgga cctcggctgg tcctacctgg ccaccggacg cagccgctcg
     2701 gccgagggtg cctaccgcga gagccttcgc gcgctcccgc agcacggcga gcggctgtgg
     2761 ggtctggccg aggcgctgcg ccggcagggc cgctacgaag acgcgatcga ggcgtatcgt
     2821 gcgtcgctgg tgcagcgacc cgagatgttc gagagccacc tggggctcgc gctgtcctac
     2881 ggcagtctcc aacgattcga cgaggcgttc gagcacacgc gacggcaggt cgagatcaac
     2941 ccttcctcga cgatcgcgtt gaccaacctg acgagcctcg gcgtccaggt cggcgagttc
     3001 gaggcggcaa tatccgcgtc cgagcggctg gtcgagctcg acccgacgag tctcgagctg
     3061 cactttgcgc gatggaacgc gatgacgcgg agcggcaagc ccgagcggga aatcgccgcg
     3121 ttgcgcgatt cccaccggcg cttcccggat cagtggatgt tcatctgggc gctcgcctgg
     3181 cgtctggcgg tgactcggga caccgcggag gacgacctgc gcgaagcggt ccggctcggc
     3241 gagcaggccg tcgagctgaa tcccaaccac gcgcgcagct gggatgcgtt cgccgcggcg
     3301 caggcggcgg tcggcgacta cgagcaggcg gtcgcggcgg cgcgtcaggc ccacgcactg
     3361 gccgccgcct ccccggacac atcgctcgcc gggcagatcg ccgcgcgcct cgaatcgtac
     3421 gagaccgggc gaccgtacta cgaatagctc ctcgcctggc gctcagtgcc ccgcgccgat
     3481 ccgctgccgg tgctcgccga gcgccgccgc gaccgccgga cgagcctcct gcaccaactc
     3541 cgcgagggtc gcgttcatcg cttcggcgat ctcgacgact tcgtcggcat cgagcgcgcg
     3601 accgccggcg gcggcgtacg accacagctg gctgccgtcc ggcagggcca gcaccagctc
     3661 cacccgcaag cggacttcct cgcggtcggc atggtcgatt tcctcgatcg tcgtgacccg
     3721 cccgtgcaac agcgcggcgt aggcccgtcc cggcaccgcc ggctcgaacg ccgtcgcgcc
     3781 gtcggccaag gacagtccgc gagcgaagac gaccggtagc atccgttcga gcggcgccgc
     3841 ccaccggtgg taatcgtaga agccgacctc ggtcgggtga gcggcgacgc ggtagaccag
     3901 cgtgtcgcgg tcgtagggag gatcgacgcg gaaatccgtt acgccgacga cgtgcgcccg
     3961 tgcgccggta tcgcccccac ccggcccgac gggccgaggg gtctcgagca cgtagtagtg
     4021 ggtcggtggc accgacccgc atccggcgat cgcccacaga ccggcgatcg cgcacagcgc
     4081 ggcggcccac ctcatctcgc cccctggccg ggcgcgcgtt gcggcggcgc cgacatgcgc
     4141 acgagcgagt agggccgctc cttgatcgtc tgcgaaaacg ccttgaggtt ggcggcggta
     4201 tcgcgcaggt cacgcagcgc gtcgccgagc tcgtgacggt tgtcacccag caccgacaac
     4261 gcgtcgtcgg ccgaacccag actcgcgtcg gcgcgctcga gcagcgagcg caagcgctcg
     4321 ccgtcgggcc cgatcgcggt ccgtaggtcg ccggccagcc cgtcgagccg gcgggcgaga
     4381 tcgggaaggg cgtcgagctc gcgatcggcc ttcgccgcga ccgagtcgag acgcgagacc
     4441 agactcccga gctgcggacc gaactcgtcg accgcccggt tgagcttgct caacaacgcc
     4501 gtgacgttgt cgacgttctg acccgacatc aatcgattgg cgccctcgag cgcctggtcc
     4561 aggtcgacgg caatcgcgtc gagcagggac gaagtctgat cgaggagctt ggccacgcgc
     4621 tcgccgaggt cgtcgatccg ggccatcgcg tcgtccattc cgatcgtctc ctggccacgg
     4681 atctcggccc cggcgggcag caccggcaac ctcgccgtcc cgggatcgat ctcgagaaag
     4741 gccgcgccga gcagacccgc ggaggtgatg cgcgccaccg cgtcggtgtg cagcgggacg
     4801 tcggcgcgca gcgagacgtc gaagcgtacc ggccgctcgt cgccgggacg cagctcgacc
     4861 cggcggatct tgccggccgg cacgcccgct acccgaacgc ggtcaccggc ctggaccccg
     4921 ccggaatcgc gcagcgagac gaggtagacg acccgaccgt cgccccacgc gccggtgccg
     4981 gcgatccaga acagaccgga gacgaacaac ccgagcgcac cgatcacgaa cagcccgacg
     5041 cgaatgtccg ctcgcctttc gtcacgcgcc atggtcgcgc cctctcatcg cagatcgtcg
     5101 aggaaccgac ggaacttctt tccgctgtcc tcgctcggcg ccggctcgcg atcgaggaac
     5161 cggcgcacca gcggatcatc gcacgcgcga acgaccgacg gggtgtcgac gatacggaat
     5221 cgcccgcgat cgatcaaggc gatccgatcc gcgatcgcga acgcgctgtg gagctcgtgc
     5281 gtcacgacga cggtggtcac gccgaacgcc cgcttgagct ggatcatcag ccgatccagc
     5341 tcggcggcgg tcaccgggtc gagacccgcc gacggttcgt cgaacagcag cacgtccggg
     5401 tcgagtgcca gcgcgcgcgc gagcgccgcg cgtttgacca ttcccccgct cagctcggcc
     5461 gggtagaggt ccatcgcgtc ctccagaccg accatgccga gcttgatgcg ggcgacgatg
     5521 cgccgggtca atctcggcat cgcggtgaac tcttcgagcg gcagctcgac gttctcggcg
     5581 accgtccgcg acccgaacaa cgcgccgccc tggaacgcca tgccgatccg ctcgcgcagt
     5641 cgcgacagcg tgaccggatc gctgtcgaac aggtcgaccc cctggatcgt caccctgccc
     5701 gcgtccggct gctcgagacc gaccaggcag cgcaacaacg tgctctttcc gctgccgctg
     5761 ccgccgagaa tgaccagcgt ctcgccgtgg cgcacctcga gatcgatgcc ctgcagcacg
     5821 gggttcccgc cgaaccgctt cgacagaccg ctcacccgga tcacgacatc ggcggtgtcc
     5881 aacgcgctac ccccggacat agaaccacgc ggtgacgatc aggtcggcgc agatcaccag
     5941 cacgatcgag cgcaccaccg cgcgggtggt ggcgcgtccg acctgctcgg cgccgccgcg
     6001 cgtcgccaga ccctcgcgac aaccgaccag cccgatgatg caagcgaagg ccagcgactt
     6061 gaccagtccg gtcagcacgt cctcgaggac cagggcgtcg accgtgtctt cgagataacg
     6121 cccgctgccg agcccgagcg cgccgacgcc gaccagcatg ccgcccagaa tgccgaccgc
     6181 gtcggcgaag accgtcaggc acggcatcgc caccgacagc ccgaccagcc gtgggacgac
     6241 gaggtaggag gtcggatcga tccccatcgc ctcgagcgcg tcgatctcgc tcgacacccg
     6301 catcgtgccc agctcggccg cgatcgccga gccgaagcga ccggcgacga tgatcgccgt
     6361 cagcagcggc gcgagttcgc gcgacatcga cacggcgacg agactcgcta ccagatcgac
     6421 ggcttcgaac ctgcgcaact ggtaggccga ctgcagcgcc atgatcatcc cgaccaacag
     6481 gcagatcagc gagacgagcg gcagcgagcc gttgcccgcg cgcgcgagct gctcgagcgt
     6541 ttcgcgcgag cgcaccttgc cgcgaccgaa cgggttgagg accaagcggc gcgacgagtc
     6601 gagcgcgagg cgcgacatcg cgcccgcctc ggcgaacagg ttgttggccg cgcggccgag
     6661 cgcgcggatc agccccccca gcatcgagcc agtctaggtc atcagccgtc gggccgtctc
     6721 tgccggtgtc gggcagcagg catcgagcgc ctcctcgaag ccggcgaggc ggaacattcg
     6781 catcaccgac gggctcggcg agcagagcag caccggtttg ccccgttccc gtccccggag
     6841 caacgcttcg accagcaccg cggctcccga tgtgtcgatg tcggcaacct cgcccaacga
     6901 cacgaccagc cggcgggccg gtgtctcgtc gatccagcgc agaagacggc gccttagctc
     6961 gggagcctcg ggatagcgga cccggccgtg gacccgcagc gtgctcgcat cgtcgcgccc
     7021 gcggatcgcc gcgatttcgg tctcgaaggt ccgcgcctgc attcgggccc cccgcgacgc
     7081 cccggtccag cataccattc gtccgattcc cgctcgttcg cggaaccccg ccgagcgcgg
     7141 atgcgtcaga atcgacggga acgatccgga gcaagagcga aaacgacgcg aaagtcgcgg
     7201 atcggagcgg tcgattgacc gatgtcgcga ggttgctcga gcgctgtcgt cggggcgacg
     7261 atctcgcctg ggagacactc gtgcgtcgct accagtcccg cgtctacgcc gtcgcctacc
     7321 attacgtgcg ggaggccgac gaagcgcggg acgtggccca ggagatcttc gtccgcgtct
     7381 accaaaagct cgactcgttc gaccacagct ccaacttcct cgcttggttg atgcggctcg
     7441 cgcgcaatgc ctgcatcgac cgcttgcgac ggcgcgcggc gcgcccgccg gcgagcgacc
     7501 tgccgatcgg cgagtcgatc gaggtcgagt cgacggagcc cgacccggag gagcacgcgc
     7561 gccgcgagtc agagcggcga ctgctccatc gtgcgctggc cgggatgagc gaggccaatc
     7621 gcgagatgat cctgctccgc gagatccagg gtctcaagct cgaagagatc gcctcgatgc
     7681 tcggcctgcc gctgggaacg gtcaaatcgc gatcgtcgcg tgcacggatc gagctggcct
     7741 cgcgcgtccg cgcgatcgtg cccgccttcg gggcgtcgtc gtgagtcgct tcgactgcga
     7801 tgcgttcgag cgacagctcg aggcgctgct cgacggacga tcgagcgcgg cggcgcgcgc
     7861 gcgtgccgtg cggcacctcc gcgtctgcgg cgactgcgcc gagctgatgc gactggctac
     7921 gatgccgccg ccggggccgg tcgatctcgt cgacggcgtg atggcacgga ccgccgggtc
     7981 cgcctgcgct cccgtccgcc gccggctgtg cgctctcgtc gacggcgagc tcgatccgct
     8041 ccaggccgag caggcccgat cgcatgtcga ctcctgtgcc gattgcgcga cgctcctcgc
     8101 ggtgctcggc gcgttgcccg ccgcgctcgt gctgttgcgc gagatggcgc tggacgatcg
     8161 cttcacggcc gacgtgctcg cccgcatccg gtcgttgccg agcaaagcgc cggggcgtac
     8221 gccgtcctgg ctccggcgcg ccgtgcagct gctcgagcgc ccgcgtttcc cgctcgaggc
     8281 ggcctacgtg atgacgacgc tcgtggtgct gttcgccggg atcccgagcg caccgatcgc
     8341 cggggtgtcc cgccgcgcgt tcgagatcgc gtcggccgat ctgcccgccg gcatcgagca
     8401 gtccgtcgtc ggggtcggtg accgggtcgg cggaagcgcg aagcaaacct gggattccgc
     8461 cagcgagcag gtcagccgcg acgcccgtcg ccgggcagac gccgttgccg agctttcgtc
     8521 cgtcttggcg agcgatatcc gcaccggcat cggaaccttg tgggagcgga ttgcgtcagt
     8581 agagggggaa caggacgaca ggttgtcgcc ggccgagaac gacggccgaa cgggagatgg
     8641 acgatgaaca cgcaagccga tcacaacccc cgaccgagcc tcgaagaccc cggacggaag
     8701 tcggtggtcc tggcggtggt gctgtcggcg ctcccgggcc tggggcaggt ctacgtcggc
     8761 tactatccgc ggggattcac ccactcactg gtcgcagcga gtctgatcgc ggtcctgtcg
     8821 tcgttgacgg agttcagccc gttgttcccg ctcgggatga tctttctctt ctttttctgg
     8881 ttctacaaca tgatcgacgc cggccggcgc gcggcgatgt acaaccacgt actggcgggt
     8941 ggcgagatcg tcgacctgcc gaaggacatc agcattcccg gtctgcgcgg ctcgatcgtc
     9001 ggcggcttgg tgatcatcgc cgtcggcgcg atcgtgctgt cgcacacggc ctacgacgtc
     9061 ccgctcgatt ggctggagga ttggtggccg gcgggggtcg tgctgttcgg cgtctacctc
     9121 gtggtcaagg gggtccagga acgcgtgggt acgtcgctcg gctgacgccc ggcgcgagaa
     9181 acgagaacgg cccgcggggc ttctcccccg gcgggccgca ctcgatcgac cgaacgggcg
     9241 cagccgccct agctcgccgc cttggccgtc tggtgctgga gctccttggt gaccgccacg
     9301 tcctcccgct tcccctgctc ctgccgccgg gcgctgctcg cgacgtccat gtcgatgctg
     9361 cccttgaaca tcgcgccatc cgagatcgag acgcgcgggg cacggatgtc gcccatcacc
     9421 gtgccggagt tggcgatctc gaccttgttg tcggccgtga tgttccccgt caccttgccg
     9481 tggacgatca ccgccttggc ctgcagctcg gcgtcgatct gaccgttggc gccgatcgtc
     9541 agcacgtgct ccttgagcgt gatcttgccc tcgacccgac cgtcgacggt gaggtcctca
     9601 cggccggaga gcgtgccctt gatgtggacc
//
LOCUS       DASPGN010000298         7115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1108165, whole genome shotgun sequence.
ACCESSION   DASPGN010000298 DASPGN010000000
VERSION     DASPGN010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108165"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="VD788_08660"
     CDS             <1..966
                     /locus_tag="VD788_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76378.1"
                     /translation="NHAYKPYRDRVSANRDFLVSLLDVVAGEPAHLIDAVDRADRRTV
                     AMGAADARPSSIALSYEAEQPRDRIRFPVYEWSKVDSLVIGDEIVDYAPGKLREIEVP
                     WSHRPRVVRSASRPRGYLVLPGWPDIERRLADHGLRVRRVIEPTELEVETIRLTVEHR
                     EAPASASYQGLTRLPVTAERRRERRSVPAGSLWIPADQPDFEIAVQLIEPEAPDSLAA
                     WGLLSIVTERKEYIDLRLLERLARGLLEDPTIRAEWEKAVEDPAFAADAGARWTWWYR
                     RTKFWDETVGLLPVYRVPSRPSLTTEPWSGAAWSVAPPASAEGVR"
     gene            996..1661
                     /locus_tag="VD788_08665"
     CDS             996..1661
                     /locus_tag="VD788_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76379.1"
                     /translation="MDTIRFQTWWRQFERRVEGEGCHEIARRLRAKLRRTRVADRPEF
                     VRELWDKLLQRQHSYGVALLVLGGLSELERLRELARRLTPLPPPQPADEEAHLADLMR
                     ILAASNDDELLRPVETYLLERPISPYWATVPWALWPHRKRLFARAWQRYLQDVQPTDW
                     KPTEVLECFLAEPDAVRLVRTTISDNHPEHWNDLRDALIGEADQVGWLSVEQRESLDR
                     VIY"
     gene            1658..2431
                     /locus_tag="VD788_08670"
     CDS             1658..2431
                     /locus_tag="VD788_08670"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HXV76380.1"
                     /translation="MNRFAPFGDRVWTIAHRGASRDRPENTLVAFDEALRQGCDAIEL
                     DLQLTRDGAVVVFHDLTLARAGGGRRSLRSIDRAGLVSLGLAVPTLDEVLERYSGPTW
                     LLLEIKQTRTERALDEIVRQTVDAVARYGARERVFLLSFDRAALERSADLDPGLRRVL
                     NVGGRDPVDRFPAAFAGLHAVSVDVRALRPPLLAAVEAARLPLLVFTCNQTGEVERAL
                     AAGAAGVMSDRPGWLRGYLAARSAAGDGASVACGDRPIE"
     gene            complement(2399..3433)
                     /locus_tag="VD788_08675"
     CDS             complement(2399..3433)
                     /locus_tag="VD788_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXV76381.1"
                     /translation="MPRHNFAEPRGTIERWTLHSGALRANRLGDPADRTVAVYLSPGY
                     DPAGEPSPVFVGLAGFTSGGLKLLGWQSFGESLPQRIDRLIDQGELGPLVVVLPDCFT
                     SLGGNQYVNSPVVGRWEDFLLHEVLPEVERRYHVRSDAVGRAVFGKSSGGYGALIQAL
                     RHGDCWGAVACHSGDIDFDLCYRPQLAQVVDVLAGFDGDAARFIEHVRRATKIAGHEM
                     FALMMLAMCATYDPDPDAAFGVRLPVDARTCALIDDRWQHWLRHDPLRIVEQPAARNN
                     LTRLRLLFLDCGSRDQFAAHFGARRFAARLAQHEIAHVFEEFDDDHSGIDYRLDRSLP
                     LLYRAVTAGD"
     gene            complement(3438..5372)
                     /locus_tag="VD788_08680"
     CDS             complement(3438..5372)
                     /locus_tag="VD788_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXV76382.1"
                     /translation="MRIRTSCNRDCPDACGIVATVDGGRVTRIEGDADHPVTRGFLCY
                     RTNRFLDRQYDPDRVTTPLLRRTDGFVAIDWDEALDLVAAKMLEIRAESGAAAILHYR
                     SGGSLGLMKHLSDFFFERFGPVTIKSGDVCSGAGDAAQLEDFGEEDSHDLYDLYNSRT
                     IVLWGKNPYVSSVHLLPVLREARARGTRLILVDPVQHRTVGICDWYLQPRPGGDIALA
                     LGVARLLFERGLADADCGSYCDHVDEFRELCFSRGTEQWAALADVAPADLDRLASAFA
                     DGPLAILVGWGMQRRTYGSATVRVLDALGAVSGNIGVAGGGVSFYFKRRGAFDLSFAS
                     GLAAAPRSIPEPLLGPGILAASAPEIRMVWVTAGNPVAMLPESATVARALRTREFTVV
                     VDSFITDTAECADLVLPTTTMLEDDDLVGAYGHHYLGNIRPVVPPPPGVKTDYEIIRQ
                     LAPRVGLNGEFSLPSREWKRRLLQRVEPLGVSLESLEQRPMRNPLTAAVLFADRKFPT
                     ASGKANLIRTVDPEPVRPTSDRPMLLMAISTDKAQGSQWRSTQQHGPPWLTVHPASAA
                     GLADGGYAVLESSTGAMRVRLRFDGGQRRDVALIAKGGWFGRGRCANVLVAAKTTDAG
                     GGAAYYDTPVRLLPDLDPRA"
     gene            5450..6754
                     /locus_tag="VD788_08685"
     CDS             5450..6754
                     /locus_tag="VD788_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HXV76383.1"
                     /translation="MSVLNLVSLAGIAVLSLVAWLAGGCRRPVPLRTVRGSLVLMIAT
                     GTLVFLVPQSRQLLVRLNDLVVAALGSANAGAEFLFGPLAVGPGRSTAAGESSIGFVL
                     AAQVLPAVVFFTALMALFYFLGLVQPVVRVFGRLFHRSLGLSGAESLSGAANIFLGVE
                     SVTTIRPYLDGLTRSELLTVLTCCMSTVASTTLAIYVLFLDDVFPRIAGHLISASVIS
                     IPAAALVSKLMLPETERPATRGHVPPLPRDDRAENPVAALTAGAWDGLRLAAGIATLL
                     IAVLGIVGLVDLGLNRISQPFADRLGGVIDVGRILGWVFTPLTLLLGVEPADVSQAGR
                     LLGQRLVVTEVPTYRELAELSAAGAISPRTMLILSYALCGFAHVASVGIFVGGISAIA
                     PTRRADLAALAPRALLGATLATLLTGALAGLFYHGQGGVLAG"
     gene            complement(6759..>7115)
                     /locus_tag="VD788_08690"
     CDS             complement(6759..>7115)
                     /locus_tag="VD788_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76384.1"
                     /translation="SHLIGCHFHNSVLAGGAKLSDCTIEQSVVGIRTVMNRATVKRAL
                     IMGAEDDPPRPNLVGVRDGALIQDAIVDLNACIGRGAKIVNEERLQEASGDGWVIREG
                     IVVVLKNAVIADGTVI"
BASE COUNT         1019 a         2484 c         2472 g         1140 t
ORIGIN      
        1 aaccacgcct acaagcccta tcgcgatcgc gtgtcggcca accgcgattt cctggtttcg
       61 ctgctcgacg tcgtcgccgg cgagccagcg cacctgatcg acgccgtcga ccgtgccgac
      121 cgccgaacgg tggcgatggg agccgccgat gcccgaccgt cgagcatcgc gctgtcctac
      181 gaagccgagc agcctcgcga ccggatccgc tttccggtgt acgagtggag caaggtcgat
      241 tcgctggtga tcggcgacga gatcgtcgat tacgccccgg gcaagctgcg cgagatcgaa
      301 gtgccgtgga gccaccgtcc gcgcgtcgtt cgatcggcca gccggccacg gggttatctc
      361 gtcctcccgg gctggccgga catcgagcgc cggctcgcgg accacgggct gcgcgtgcgg
      421 cgggtgatcg agccgacgga gctcgaggtc gagacgatcc ggctcacggt cgagcaccgt
      481 gaagcgcccg cctcggccag ctaccagggg ttgacgcgct tgccggtcac cgccgagcga
      541 cgccgcgaac gtcgctcggt gccggccggt tcgctgtgga tcccggccga tcagcccgat
      601 ttcgagatcg ccgtccagct gatcgagccg gaggcccccg actcgcttgc cgcctggggc
      661 ttgttgtcga tcgtgacgga gcgcaaggag tacatcgacc tgcggctgct cgagcgtctc
      721 gcgcgcgggc tgctcgaaga cccgacgatc cgcgcggagt gggagaaagc ggtcgaggac
      781 ccggcgtttg ccgcggacgc cggcgcccgc tggacgtggt ggtaccggcg gaccaaattc
      841 tgggatgaaa cggtcggtct gctgccggtc taccgcgtcc cgtcccgtcc gtcgctgacc
      901 accgagccgt ggtcgggcgc ggcttggtcg gtcgcgccgc ccgcgtcggc tgaaggggtg
      961 cgctagagta ttcggggtga ggcttgcggt ctgaggtgga caccatccga ttccagacct
     1021 ggtggagaca gttcgagcgc cgcgtcgagg gcgagggctg ccacgagatc gcccgccggc
     1081 tgcgcgcgaa gctccgacgc acccgggttg ccgaccggcc cgagttcgtc cgcgagctgt
     1141 gggacaagct gctgcagcgg cagcactcct acggcgtcgc gttgctcgtg ctcggcggcc
     1201 tgagcgagct cgagcgactg cgcgagctgg cgcgacggct gacgccgctg cccccgccgc
     1261 aacccgccga cgaggaggcg cacctcgccg atctgatgcg aatcctcgcc gcgtcgaacg
     1321 atgacgagct gctgcgtccg gtcgagacct acctgctcga acggccgatc agcccgtact
     1381 gggccaccgt gccgtgggcg ctgtggccgc atcgtaagcg gctcttcgcc cgcgcctggc
     1441 agcgctacct gcaggacgtg cagccgaccg actggaagcc gaccgaagtg ctggagtgct
     1501 tcctggccga gccggacgcg gtccggttgg tgcgcacgac gatctccgac aaccatcccg
     1561 aacactggaa cgacctgcgc gatgcgctga tcggagaagc ggaccaggtg ggttggctgt
     1621 ccgtcgagca gcgcgagtcg ctggaccggg tgatttattg aaccgattcg cgccgttcgg
     1681 cgatcgggtg tggacgatcg cccaccgcgg cgcgtcgcgt gatcgaccgg agaatacgct
     1741 cgtcgcgttc gacgaggcgc tgcgacaggg ctgcgacgcg atcgagctcg atctgcagct
     1801 gacgcgggac ggcgcggtgg tcgtgtttca cgacctgacg ctcgcgcgcg caggtggcgg
     1861 gcggcgcagc ctgcgctcca tcgatcgcgc ggggctcgta tcgctcggtc tggccgtgcc
     1921 gacgctcgac gaggtgctgg aacgctactc cggtcccacg tggctgctgc tcgagatcaa
     1981 gcagacccgc accgagcggg cgctcgacga gatcgtgcgc caaacggtcg atgccgtcgc
     2041 ccgctacggc gcccgcgagc gcgtcttcct gctgtcgttc gaccgtgcgg cgctcgagcg
     2101 ctcggccgat ctcgacccgg gtctgcgccg tgtgctgaac gtcggcggcc gcgacccggt
     2161 cgaccgtttt cccgcggcat ttgccggttt gcacgccgtc tcggtcgacg tccgcgcgtt
     2221 gcgcccgccg ctgctcgcgg cggtcgaggc cgcccggctg ccgctgctgg tgttcacctg
     2281 caaccagacg ggcgaggtcg agcgcgcact cgccgccggc gcggcaggag tgatgtcgga
     2341 tcggcctggt tggctgcgcg gctatctcgc cgcgcggtcg gccgcgggcg atggcgcgtc
     2401 agtcgcctgc ggtgaccgcc cgatagagta gcggcagcga tcgatcgagc cgatagtcga
     2461 tccccgagtg gtcgtcgtcg aactcctcga agacgtgggc gatctcgtgc tgcgcgagcc
     2521 gtgccgcgaa acgccgagcg ccgaagtggg cggcgaactg atcgcgcgag ccgcagtcga
     2581 ggaacagcag ccgcaagcgg gtgaggttat tgcgcgccgc cggctgttcg acgatccgta
     2641 gcgggtcgtg ccgtagccag tgctgccatc ggtcatcgat cagcgcgcag gtgcgtgcgt
     2701 cgacgggcag tcgcacgccg aaggccgcgt ccggatcggg gtcataggtc gcgcacatcg
     2761 ccagcatcat caacgcgaac atctcgtggc cggcgatctt cgtcgcccgc cggacgtgct
     2821 cgatgaagcg cgcggcgtcg ccgtcgaagc cggccaacac gtcgacgacc tgcgccagct
     2881 gcgggcggta gcacagatcg aaatcgatgt cgcccgagtg acacgccacc gcaccccagc
     2941 agtcgccgtg tcgcagcgct tggatcaacg caccgtagcc accgctcgac ttgccgaaga
     3001 cggcgcgccc gacggcatcg ctccgcacgt ggtatcgccg ttcgacctcg ggaagcactt
     3061 cgtgcagcag gaaatcctcc cagcggccga ccaccgggga gttgacgtac tggttgccgc
     3121 cgagcgacgt gaagcaatcg ggcaggacga cgacgagcgg ccccaactcc ccttggtcga
     3181 tcagccggtc gatgcgctgc ggcagactct cgccgaacga ctgccagccc agcagcttca
     3241 acccgccgga agtgaacccg gcaagcccga cgaagaccgg cgacggctcg cccgcggggt
     3301 cgtagcccgg cgacaggtag accgccaccg tacggtccgc cgggtcgccc agccgattgg
     3361 cgcgcagcgc gccggaatgc agcgtccaac gctcgatcgt gccgcgtggc tcggcgaagt
     3421 tgtggcgtgg cacgctgtca ggctcgcggg tcgaggtccg gcagcagccg caccggggta
     3481 tcgtagtagg cggcgccgcc gccggcgtcg gttgtcttgg ccgcgaccag cacgttcgcg
     3541 catcgcccgc gtccgaacca cccgcccttg gcgatcagcg cgacgtcgcg ccgctgccct
     3601 ccgtcgaacc gcaggcgcac gcgcatcgct ccggtcgagg actcgagcac ggcgtagccg
     3661 ccgtcggcca gaccggcggc cgaggccggg tgcacggtca gccacggcgg gccgtgctgc
     3721 tgcgtcgagc gccactgcga cccctgggcc ttgtccgtcg agatcgccat cagcagcatc
     3781 ggacgatcgc tggtcgggcg caccggctcc ggatcgacgg tgcggatcag gttggccttg
     3841 ccgctggcgg tcgggaactt gcgatcggcg aacagcaccg ctgcggtcag cggatttcgc
     3901 atcggtcgct gctcgagcga ctcgagcgag actcccagcg gctccacgcg ttgcagcaaa
     3961 cgccgcttcc actcccgtga aggcagcgag aactctccgt tcaagccgac gcgcggcgcc
     4021 agctgacgga tgatctcgta gtcggtcttg acccccggtg gcggtgggac gaccggacgg
     4081 atgttgccga ggtagtggtg gccgtacgcg ccgacgagat cgtcgtcctc gagcatcgtc
     4141 gtggtgggaa gtacgagatc ggcgcactcc gcggtatcgg tgatgaagct gtcgacgacc
     4201 acggtgaact cgcgcgtgcg caacgctcgc gcgaccgtcg ccgattccgg cagcatcgcc
     4261 accgggtttc cggccgtgac ccagaccatg cggatctccg gtgcggatgc ggcgagaatc
     4321 cccgggccga gcagcggctc ggggatcgag cgcggcgccg ccgcgagacc gctggcaaag
     4381 gaaagatcga acgcaccgcg gcgcttgaaa tagaaggaca cgccgccgcc cgctactccg
     4441 atgttgcccg acaccgcgcc gagcgcgtcg agcacgcgca ccgtcgccga tccgtaggtc
     4501 cgccgctgca tgccccagcc gaccaggatc gccagcggac catcggcgaa cgccgaggcg
     4561 agacggtcga gatcggcagg ggccacgtcg gccagcgccg cccactgttc ggtgcctcgc
     4621 gagaagcaga gctcgcggaa ctcatcgacg tggtcgcagt agctgccgca gtccgcgtcg
     4681 gcgaggccgc gctcgaagag caaccgcgcc acgccgagcg ccagcgcgat gtcgccgccc
     4741 ggccgcggct gcagatacca gtcgcagatc ccgaccgtgc ggtgctggac cggatcgacc
     4801 aggatcaagc gggtaccgcg ggcgcgtgcc tcgcgcagca ccggcagcag gtggacgctg
     4861 ctgacgtagg ggttcttgcc ccacagcacg atcgtgcggc tgttgtaaag gtcgtacagg
     4921 tcgtggctgt cttcctcgcc gaagtcctcg agctgcgcgg cgtcgccggc ccccgagcag
     4981 acgtcaccgg acttgatcgt caccggtccg aagcgctcga agaaaaagtc ggagaggtgt
     5041 ttcatcaacc ccaacgaccc accgctgcga tagtgcagta tcgccgccgc gccggactcc
     5101 gcacgaatct cgagcatctt ggcggcgacc aagtcgagtg cctcgtccca gtcgatcgcg
     5161 acgaacccgt cggttcgccg cagcagcgga gtggtgacgc gatccggatc gtactggcgg
     5221 tcgaggaagc ggttggtgcg gtagcagaga aaaccgcgcg tcaccgggtg gtcggcgtca
     5281 ccctcgatcc gcgtcaccct tccgccgtcg acggtggcga cgatcccgca cgcatcgggg
     5341 cagtcacgat tgcaactggt ccggatcctc atgccgggca ggataacgga ggcgcagccc
     5401 cggctcaaat ggctgccgcc cgccgctcga aagtccgata atggtccgca tgagcgtcct
     5461 caacctcgtt tcgctcgccg gcatcgccgt gctgtcgctc gtcgcctggc tcgccggcgg
     5521 ctgccgccgg ccggttcccc tgcgaacggt acgcggctcg ctggtgttga tgatcgcgac
     5581 cgggacgctg gtctttcttg tcccgcagtc gcgccagctg ctggtccgcc tcaacgacct
     5641 cgtcgtcgcg gcgctcggca gcgccaacgc cggtgcggag ttcctgttcg gcccgctggc
     5701 ggtcggcccg ggccggtcga ccgccgccgg cgagtcgtcg atcggcttcg tgctcgccgc
     5761 gcaggtgctc ccggcagtcg tgttcttcac ggcgctgatg gcgctcttct acttcctcgg
     5821 tctggtccag ccggtggtgc gcgtctttgg ccgcctgttt catcgcagtc tcgggctgtc
     5881 cggcgccgaa tcgctgtcgg gcgcggccaa catctttctc ggggtcgagt cggtcacgac
     5941 gatccgtccc tacctcgacg gcctgacccg gtcggagctg ctgaccgtgc tgacctgttg
     6001 catgtcgacc gtcgcgtcga cgacgctcgc gatctacgtc ttgtttctcg acgacgtttt
     6061 tccgcggatc gccggtcacc tgatctcggc ttccgtcatc tcgatcccgg cggcggcgct
     6121 ggtctccaag ctgatgctgc ccgagaccga gcggccggcg acgcggggtc acgtcccacc
     6181 gctgccgcgc gacgaccgcg cggagaaccc ggtcgccgcg ctgaccgccg gtgcgtggga
     6241 cggtctcagg ctcgccgcgg gtatcgcgac gttgctgatc gcggtgctgg ggatcgtcgg
     6301 gctggtcgac ctcgggctga atcgcatcag ccagccgttc gccgaccgcc tgggtggggt
     6361 gatcgacgtc gggcggatcc tcggctgggt gttcacgccg ctgaccctgc tgctcggcgt
     6421 cgagccggcg gacgtgagcc aggccggccg cctgctcggc caacgcctgg tcgtgaccga
     6481 ggtcccgacc taccgggagc tggccgagct gtcggccgcc ggtgcgatct cgccgcgcac
     6541 gatgctgatc ctgtcgtacg cgctgtgcgg tttcgcccac gtcgcatcgg tcggcatctt
     6601 cgtcggcggg atttccgcga tcgctcccac gcgacgcgcc gatctcgccg cgctcgctcc
     6661 gcgggcgctg ctcggcgcga cgctcgccac gctgcttacc ggggcgctgg cgggcttgtt
     6721 ctatcacggg cagggcggcg tgctcgccgg atgacccgtc aaatcaccgt gccgtccgcg
     6781 atcaccgcgt tcttcagcac cacgacgatt ccctcgcgga tcacccagcc gtcgccgctg
     6841 gcttcctgca accgctcttc gttgacgatc ttcgcgccgc ggccgataca ggcgttgagg
     6901 tcgacgatcg cgtcctgaat cagcgcgccg tcgcgcacgc cgaccaggtt cgggcgcggc
     6961 gggtcgtcct cggcgcccat gatcagggcg cgcttgaccg tggcccgatt catcaccgtg
     7021 cggatcccga cgaccgactg ctcgatcgtg cagtcgctga gcttggcgcc cccggccagc
     7081 accgagttgt ggaagtggca cccgatcagg tgcga
//
LOCUS       DASPGN010000299        13352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_522657, whole genome shotgun sequence.
ACCESSION   DASPGN010000299 DASPGN010000000
VERSION     DASPGN010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 13352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13352
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522657"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2133
                     /locus_tag="VD788_08695"
     CDS             <1..2133
                     /locus_tag="VD788_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76385.1"
                     /translation="FTTVVFYTAPGSGDGAPRDRMIVDTDFVRNARLFDPLRETELRV
                     GSTPVDAPSRGRAVRIARSSYPQAVVFERPAADLPPEAIESRRGRELTAEEIIARFQQ
                     VDKIQSDRLERWMATGRIDFHFRFVQGGSTIDVSIDTFYFWERGGDLEWEELDYYING
                     NLVPWKNFPKIPLIQPEKVITLPLDLTLDKTYVYERHGIERVDGREAYVIGFRPAAPA
                     NDRSLYRGRVWIDTVDFNRLKASLTQTQMESPILSNEEIDLFATRRDAAGNEYQLLDR
                     IDGQQVWNTAGRSFTVRREVTFHEYTVNPPRERFEQRRDQAYASKNNIIRDTDEGFRY
                     LERNADGTRELKPLETSQWFAAVGAFESGSTDGVQPFGGANYFNFDLFGDNVQINALI
                     GGVVNFLTLSKPDLARGKLSLTSDLFVSALYFNDTVFADGVELEAERIDQRSQSVNLR
                     LSWNASQFVKLNVIGAAGYREFDQDDEAADARAAYNAATGQQLRFILPQDHTELAATF
                     ELEFSRRAYSMVAGYTEGRRSDWTEWGLFDESTGGFVVYDPLSGLYLPGPAPTVESSF
                     RLWGIDAFKEWYLPHFQKVRTEVNYLDGADQDRFSQYQFSLFGNDRLSGFAGTGVRFD
                     RGIIGRAGYSFNLFEVVRLDAVAENAWIRDPLSSSETQSHTGVGLAANFVAPWKLVIS
                     ASYGRAIASDNPELVGSDEFFVLILRLF"
     gene            complement(2130..3224)
                     /gene="wecB"
                     /locus_tag="VD788_08700"
     CDS             complement(2130..3224)
                     /gene="wecB"
                     /locus_tag="VD788_08700"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:NF014411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HXV76386.1"
                     /translation="MKLVNVVGARPNFIKIAPLMSAYATHAQIRPLLVHTGQHYDAAM
                     SELFFRQLGIPEPDINLEVGSASHAVQTAEIMRAFEPVVLEHRPDAVLVVGDVNSTIA
                     CGLVAVKLGVRLIHVEAGLRSRDRTMPEEINRMLTDAISDLLFCTEQSGVDNLLAEGV
                     AAEKIFLVGNVMIDTLLRHRRAAEQSTVLERLGLVDGGYAALTLHRPSNVDDPAVLSG
                     ILDALEVVQHDMPVVFPVHPRTRNALARSPLGRRAEAMGGLRLIDPLGFLDFLKLTSA
                     ARCVLTDSGGIQEETTILGVPCLTLRENTERPITAELGTNRVVGTDPARILAAYRAVI
                     AEQTPRATAPPLWDGRAAERIVEILLRELR"
     gene            complement(3221..4486)
                     /locus_tag="VD788_08705"
     CDS             complement(3221..4486)
                     /locus_tag="VD788_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV76387.1"
                     /translation="MRVLFFSHYFPPEGNAPASRTYENCRRWVEDGHRVTVVTCAPNH
                     PSGVLYPGYDNRWREREQVDGIEVVRVKTYLAANQGTVRRILNYLSYLASAVLASLDA
                     ERPDVIVATSPQFFCGWAGVLAARLHRSPLVLEIRDIWPESITAVGAMRSRPLLRVLE
                     WLELRMYDAATQIVTVGDGYRRCLLEKGVAGTRISVVMNGIDRRLFSPREPDRRLIER
                     LGLVGRFVVSYSGTIGMACGLEVVLDAAAELNARGRREVVFLLVGDGARRESLEREAA
                     RRQLDNVRFTGRLDKSQMPAVLALSDVCLVHLRRTPLFETVMPSKIFEALGMRRPVIV
                     GVAGDATRLVERAGAGIPIVPDDAGQLVEAVERLARDAALRERLGRDGERYVITHHDR
                     DALARDYLDLLARVAAMHGPGASRRGSTG"
     gene            complement(4494..6203)
                     /locus_tag="VD788_08710"
     CDS             complement(4494..6203)
                     /locus_tag="VD788_08710"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HXV76388.1"
                     /translation="MRRGSQEASVALLWHTARHLRARQLLAQLWRRSGARLGDPQRLA
                     RRERPRYPGCAWHLAHRPLAPGRQRNDAQRLLGGRFEFINEPVDAGWPPDWRCDDQSL
                     LWRYNLHYFEWLWALDFDDARHAVDSWIAHHPPGRRAVGWAPYPLSLRLQNWLALFFG
                     RWRERTERERPWAERLWSSVWVQAEWLARNLEYHLMGNHLLENAVALTWAGACFAGDD
                     AGRWFRTGLRLLRRELDEQILPDGMHYERSPMYHLRAAYALLLLSCVERRELAPLLCP
                     VLERMSRATGMLMHPDAGIALLNDSAFGIANEPTELLAAAAERQAGCAALPHGAWSLP
                     AGGYYGYRDGGTYLICDAAPIGPDHMTGHAHGDLLSFELSAGGRRMLVDSGVSSYAAG
                     EPRSFSRSTRAHNTVEIEGQDQCEFWGVFRVGRRGRPFDVDWCGDDDGFALGASHDGY
                     RRLPGAPVHRRLVSYDRRRRVLEVIDRVEASRPVRAVARLHLHPACELGQRQDDSAEI
                     VRDDQLLRLVYDGSPGAIEREPSWYMPEFGRRIPRICLARTAHGSSIDLRLRIELAGR
                     SGG"
     gene            complement(6200..8353)
                     /locus_tag="VD788_08715"
     CDS             complement(6200..8353)
                     /locus_tag="VD788_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HXV76389.1"
                     /translation="MIQILQHLRSGRLELAELPDPQLRSGSVLIQTRASVISAGTERM
                     LVEFSRASLLQKARQQPDRVRQVLDKMRTDGLLPTLEAVFRKLDEPLPLGYCNAGVVL
                     ETGRDVHDLSPGDRVASNGPHAQLVCVPRNLCARIPDGVTDEHAAFTVLSAIALQGIR
                     LADPTFGERFAVFGVGLLGLLTVQLLRASGCQVLAVDPDTARLQLAERLGARTVDLSA
                     GGDPIAAAKAWTADRGVDGVLITAAAETDEIVHQAAQGCRKRGRIVLVGVVGLDLRRS
                     DFFEKELTFQVSCSYGPGRYDERYEQLGQDYPYGFVRWTEQRNFGAVLAAMHNGTLRV
                     DPLITDRLPFDRAIEAYDKIRGDANTLGVVLRYSPEVKRTPSVAGTVRPSSAVNGVVA
                     GVIGAGNFAKGVLIPALAATSAELAYVASRTPASARHAAAKFGAGQAVSDHRLVLDDR
                     RVNAVFIVVGHDQHARFACEALAAGKHVFVEKPLALDVAQLGELTRAVAAAPGLQLAV
                     GYNRRFSAHTQKARALLAARSGPLCMSITVNAGEIPSDHWIQDAARGGGRIIGEACHF
                     IDLLSYLAGCEIRTVGAQMVGPGPAVREDKMTIVLGFADGSLGTVHYFANGPRGYPKE
                     TLECFADGRALRVENFRVTRGFGYRSLRRFRTARQDKGHRAELAAFVDRVRHGGEPLM
                     PFDGLLNVSLASIAAVRAARERAIVEIASIAGEVG"
     gene            complement(8350..9774)
                     /locus_tag="VD788_08720"
     CDS             complement(8350..9774)
                     /locus_tag="VD788_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76390.1"
                     /translation="MDLEKLYLALPIPLQNLGVTATGWRIRRRRFDREFRQLWAAYDE
                     RTGWDRLRVSRLRDRRLAEFVQRAARDVPHYRELFERLRIDPREIGGLEELSVLPVLT
                     KEEVQRAPERFRSPAIGRRRLLAAHTSGSTGAGLRFVTTWSAHREQWAVWWRYRGWHG
                     LRPGTACLYMGGRSIVPVRQRRPPYWRRNHAGSQTLFSAYHLNSDTAGDYLREMRDSG
                     APWIHGYPSMVALLASFSLEFGIRVPVRWVTLGAENLLPSQARVIERAFGVEPIEHYG
                     MAEAVANVSMCPERRLHVDEDFAATEFVPDDAGRLRVVGTNFTNPAFPLIRYEVGDHA
                     TLDEVECGCGRPGRTVARLDGRLEDYVVTKSGALLGRLDHIFKDLERVREAQIRQSEP
                     GRIVVAVVRGPGWGEPDERRLRGEILKRVGDQLDFDIVYPDSLGRTAREKLRFVISTM
                     ERGKLAAPVGRTPDATARTDEESR"
     gene            complement(9778..11145)
                     /locus_tag="VD788_08725"
     CDS             complement(9778..11145)
                     /locus_tag="VD788_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXV76391.1"
                     /translation="MSDVRLRPLGGYDRTAELRRFIAETIDGAPGTRALFSRADPRRL
                     VVVKPNWIQQSHEDRPGVWEPVITNPRLVVAAVEVVAERLDGIGTICLCDAPHTYADF
                     LAIVARGNLPAELDSIRSRWPQLRVELIDLRRETWARKEQVVVERRPNPEDPRGYVRL
                     NLGRDSLFYRHRGEGRFYGADYDSAVVNEHHRGEIQEYLLAGSPLACDLFVNLPKLKT
                     HKKTGLTCCLKNLVGINGDKNWLPHHTEGSPRDGGDEFPSGAVVHTVERELKKAGRRL
                     ALNLPGVGTWLYRKARNVGKQVLGDSHEVVRNGNWHGNDSCWRMALDLNRALLYGNRD
                     GSWRSRGQAKPYVAIVDGIVGGEGNGPLCPDPVDSRVLIGGDDPAAIDAVACRLMGFD
                     PRSLPIVAGSFETHRWPIAAKPMESLRVRDERIGADVALDRVEPAVPGGFRPHFGWTV
                     LRSSA"
     gene            complement(11168..12241)
                     /locus_tag="VD788_08730"
     CDS             complement(11168..12241)
                     /locus_tag="VD788_08730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV76392.1"
                     /translation="MAVVAEAWGRSGGIAQFNRNLIEAVADRPAVELSVLALLGATDG
                     APAAVDVRVPAPGSKTGFARAALVLARRARPELILNGTVGFGPLALALKAIGRARLWT
                     TSHGIDVWQPGGWLDNLGLRRSDLVTTVSEYTRRRLCEWSGIPTARVRVLHNAIDLAR
                     YRPGPKPERLVARYGARGRKVMLTVGRLSAGERYKGHDRVIPLLGRLAERVGPVLYVI
                     AGSGDDRARLERLVDSCGVSALVRFAGFVPDEELPEHYRLADVFVMPSTGEGFGIVFL
                     EAMACGCPVIAGNRDGSVDALAHGELGRLVDPDSPGELLEAIAQALAEGRSQERAVPG
                     VERFAVARFRERVAELVESLRGG"
     gene            complement(12341..>13352)
                     /locus_tag="VD788_08735"
     CDS             complement(12341..>13352)
                     /locus_tag="VD788_08735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV76393.1"
                     /translation="DDAPFNGLPAGRWLERDGFRACYVAGPAELARVASEVRYDAIYL
                     NSFFSAFAVRALLLRRLGRLRRVPVVLAPRGELSAGALTLKPVKKRAFLAFARWTGLY
                     RGVIWQASSDAERDEIATHTGGGARVVPNVPATARPTDGAALPGKRPGEARFVFLSRI
                     ARKKNLHFALELLGRVEGDVRFDVYGPLEDAAYVERCRGLIDGLPAGVEVRLLGPVPP
                     ERVRTALSSSQFFLFPTLNENFGHAILEALAAGLPVITSDQTFWRGLEARHAGWDLPL
                     DRADAFARVLAECVAMDAATYAARSAAARRLAAAYLDSSLAVDATRALLADACAPRDA
                     AG"
BASE COUNT         2123 a         4746 c         4552 g         1931 t
ORIGIN      
        1 ttcaccaccg tggtgttcta cacggctccc gggtcgggcg acggtgcacc gcgcgaccgg
       61 atgatcgtcg acacggactt cgtgcgcaac gcacgactgt tcgacccgct gcgcgagacg
      121 gagctgcgtg tcgggtcgac gcccgtcgac gccccttcgc gcggtcgggc cgtccggatc
      181 gcgcgatcga gctatccgca ggcggtggtc ttcgaacggc cggcggccga cctgccgccg
      241 gaggcgatcg aaagccggcg cggccgcgag ctcaccgccg aggagatcat cgcccgcttc
      301 cagcaggtcg acaagatcca gtcggaccga ctcgagcgct ggatggcaac cgggcgcatc
      361 gacttccact ttcgcttcgt gcagggcggc tcgacgatcg acgtatcgat cgacaccttc
      421 tacttctggg agcgcggcgg cgatctcgag tgggaggagc tcgattacta catcaacggc
      481 aacctcgtac cgtggaagaa cttcccgaag ataccgctga tccagccgga gaaggtgatc
      541 acgctgccgc tcgacctgac gctcgacaag acctacgtct acgagcggca cggcatcgag
      601 cgtgtggacg gtcgggaagc gtacgtcatc ggtttccgac cggccgcccc ggcgaacgat
      661 cgcagcctgt accgcggccg cgtctggatc gacaccgtcg acttcaaccg gttgaaggct
      721 tcgctgaccc agacgcagat ggaatccccg atcctgtcca acgaggagat cgacctgttc
      781 gctacgcgcc gcgatgccgc cggcaacgag taccagttgc tcgaccgtat cgacgggcag
      841 caggtatgga acacggccgg gcggagtttc accgtgcggc gcgaggtcac gttccacgag
      901 tacacggtca accccccgcg cgaacggttc gagcagagac gcgatcaggc ctacgcctcg
      961 aagaacaaca tcatccgcga caccgacgag ggattccgct atctcgagcg caacgccgac
     1021 gggacgcgcg agctgaagcc gctcgagacc agccagtggt tcgcggccgt cggcgctttc
     1081 gaaagcggct cgaccgacgg ggtgcagccg ttcggcggcg ccaactactt caatttcgac
     1141 ctgttcggcg acaatgtgca gatcaacgcg ctgatcggcg gtgtcgtcaa cttcctcacg
     1201 ctgagcaagc cggacctcgc gcgcggcaag ctgagtctga ccagcgacct gttcgtttcc
     1261 gccctgtact tcaacgacac cgtgttcgcc gacggcgtcg agctcgaggc cgagcgcatc
     1321 gaccagcgtt cgcagtcggt caacttgagg ctgagctgga acgcctcgca gttcgtcaag
     1381 ctcaacgtca tcggcgcggc gggctatcgc gaattcgacc aggacgacga ggccgccgac
     1441 gcgcgggcgg cctacaacgc ggcgacggga cagcaactgc gcttcatcct gccccaggac
     1501 cacaccgagc tggcggcgac cttcgagctc gagttctcgc gacgagccta cagcatggtg
     1561 gcgggctaca ccgagggccg ccgatcggac tggaccgagt ggggcctgtt cgacgagtcg
     1621 accggagggt tcgtcgtcta cgacccgctc agcgggctct acctgcccgg ccccgcgccc
     1681 accgtcgaat cgtcgttccg cctgtggggg atcgacgcgt tcaaggagtg gtatctgccg
     1741 cacttccaga aggtgcgcac cgaggtgaac tacctcgatg gcgccgatca ggatcggttc
     1801 agccagtatc agttcagtct gttcggcaat gaccggttga gcggctttgc cggaaccggg
     1861 gtgaggttcg atcgcgggat catcggtcgc gccggatatt cgttcaacct gttcgaagtg
     1921 gtccgcctgg acgcggtggc ggaaaacgcg tggatccgcg atccgctgtc gtcgagcgag
     1981 acccagtcgc acaccggggt cgggctggcc gccaacttcg tcgcgccgtg gaagctcgtg
     2041 atctcggcca gctacggccg ggcgatcgcc tcggacaacc ccgagctggt cggctccgac
     2101 gagttcttcg tgctgatcct ccggctgttc tagcgcagct cgcgcagcag gatctcgacg
     2161 atccgttcgg ccgcgcgacc gtcccacaac gggggcgccg tcgcccgcgg cgtctgctcg
     2221 gcgatcaccg cgcgataggc ggcgaggatc ctcgccgggt cggttccgac gacccggttg
     2281 gtgcccagct cggcggtgat cggccgctcg gtgttctcgc gcagcgtcag gcacggcacg
     2341 ccgaggatgg tggtttcctc ctggatcccg ccggagtcgg tcagcacgca ccgggcagcg
     2401 ctggtcagct tgaggaaatc gagaaagccg agcggatcga tcagccgcag gccgcccatc
     2461 gcctcggcac gccgcccgag cggcgaacgc gccagcgcgt tgcgcgtgcg cgggtggacc
     2521 ggaaagacga ccggcatgtc gtgctgcacg acctcgagcg cgtcgaggat gccgctcagc
     2581 accgccgggt cgtcgacgtt cgacggccgg tgcagcgtca gcgctgcgta gccgccgtcg
     2641 acgagaccga ggcgttcgag cacggtcgat tgctcggcgg cccgccggtg gcgcaacagc
     2701 gtgtcgatca tcacgttgcc gaccaggaag atcttttcgg cggcgacacc ttcggccagc
     2761 aggttgtcga caccgctctg ctcggtgcag aacagcaggt cgctgatcgc gtcggtcaac
     2821 atccggttga tctcctcggg catcgtccga tcgcggctgc gcagcccggc ctcgacgtgg
     2881 atcagccgca cgccgagctt gaccgcgacc agcccgcagg cgatcgtgct gttgacgtcg
     2941 ccgaccacca gtaccgcatc gggccggtgc tcgagcacga ccggttcgaa ggcccgcatg
     3001 atctcggccg tctggaccgc gtgactcgcc gagccgacct cgaggttgat gtccggttcg
     3061 gggattccca gctgccggaa gaacagctcg ctcatcgcgg cgtcgtagtg ctggccggtg
     3121 tggaccaaca acggccggat ctgggcgtgg gtcgcgtagg ccgacatcag cggcgcgatc
     3181 ttgatgaagt tcgggcgtgc tccgacgacg ttgaccagct tcatcccgtc gatcctcgtc
     3241 tcgacgcgcc cgggccgtgc attgcggcga cgcgcgccag caggtcgagg tagtcccgcg
     3301 ccagggcgtc gcggtcatga tgggtgatca cgtaacgctc cccgtcccgt ccgagtcgct
     3361 cgcgcaacgc ggcgtctcgc gccaggcgct cgacggcctc gaccaactgc ccggcgtcgt
     3421 cgggcacgat cggaatcccg gcacccgccc gctcgaccag ccgcgtcgcg tcgccggcga
     3481 ccccgacgat gaccggccgc cgcatgccga gcgcctcgaa gatcttcgac ggcatgaccg
     3541 tttcgaacag cggggtccgg cgcagatgta ccagacagac gtcgctcagc gccagcacgg
     3601 cgggcatctg gctcttgtcc agtcggccgg tgaaccgcac gttgtcgagc tggcggcggg
     3661 cggcctcccg ctcgagcgat tcacgccgag cgccgtcgcc gacgagcaga aaaaccacct
     3721 cccgccgccc gcgtgcgttc agctcggcgg ccgcgtcgag cacgacctcg agaccgcacg
     3781 ccatgccgat cgtgccggag tagctgacga cgaaacggcc gacgaggccg agccgctcga
     3841 tcagccgacg gtccggctcg cgcggcgaga acaaacggcg atcgatcccg ttcatcacga
     3901 ccgagatacg cgtgccggcg actcccttct ccagcagaca gcggcggtag ccgtccccga
     3961 cggtgacgat ctgcgtcgcc gcgtcgtaca tccgcagctc gagccactcg agcacgcgca
     4021 gcagcgggcg gctgcgcatc gccccgaccg ccgtgatcga ctcgggccag atgtcacgga
     4081 tctcgagcac cagcggcgat cggtgcaggc gcgcggccag cacgccggcc caaccgcaga
     4141 agaactgggg agaggtcgcg acgatcacgt cgggccgctc ggcgtcgagc gaggcgagca
     4201 cggcgctcgc caagtacgac agatagttga ggattcggcg gacggtgccc tggttggcgg
     4261 cgaggtaggt cttgacgcga acgacctcga tgccgtcgac ctgctcgcgt tcccgccagc
     4321 ggttgtcgta gccggggtag agcacgccgc tcgggtggtt cggtgcgcag gtcaccaccg
     4381 tcacacggtg gccgtcctcg acccaacgcc tgcagttctc gtaggtccgc gaggccggag
     4441 cattgccttc gggcgggaag tagtgcgaga agaacaggac gcgcatcgtt gcgctagccc
     4501 cccgaccgtc cggcgagctc gatgcgcaac cgcaggtcga tcgacgagcc gtgcgccgtt
     4561 cgcgccaggc agatgcgcgg gatcctccgg ccgaactccg gcatgtacca cgacggctcg
     4621 cgttcgatcg caccgggact tccgtcgtag actagacgca gcaactgatc gtcgcgcacg
     4681 atctccgccg agtcatcttg gcgctggccc agctcgcagg ccgggtgcag atggagccgc
     4741 gccacggcgc ggaccggccg cgaggcctcg acgcggtcga tcacctcgag cacgcggcgc
     4801 cgccggtcgt agctcaccag ccggcgatgc accggagcgc cgggcagccg gcgataaccg
     4861 tcgtgactcg ccccgagcgc gaagccgtcg tcgtcgccgc accagtcgac gtcgaacggg
     4921 cggccgcgcc gccccacgcg aaacacgccc cagaactcgc attggtcctg gccctcgatc
     4981 tcgacggtgt tgtgggcgcg ggtcgagcgg ctgaagctcc gcggctcacc ggccgcatac
     5041 gacgagacgc cgctgtcgac gagcatcctg cggccgccgg ccgacagctc gaaggaaagc
     5101 agatcgccgt gggcgtggcc cgtcatgtga tcggggccga tcggagcggc gtcgcagatg
     5161 aggtaggtcc cgccgtcgcg gtagccgtag tagccaccgg ccggaagcga ccacgcgccg
     5221 tggggaagcg ccgcgcatcc cgcctgtcgt tcggcggccg ccgccagcag ctcggtcggc
     5281 tcgttggcga tgccgaacgc gctgtcgttc aacagggcga tgcccgcgtc ggggtgcatc
     5341 agcatgccgg tcgcccgcga catccgctcg agcaccgggc acagcagcgg agccagttcg
     5401 cgccgttcga cgcagctcaa cagcagcagc gcgtacgccg cgcgcaggtg gtacatcggc
     5461 gagcgttcgt agtgcatccc gtcgggcagg atttgttcgt cgagctcgcg gcggagcagg
     5521 cgcaacccgg tgcgaaacca gcgcccggcg tcgtcgccgg caaaacaggc tccggcccag
     5581 gtcagcgcga ccgcgttctc gagcaggtga ttccccatca ggtggtactc gaggtttcgc
     5641 gcgagccatt cggcctggac ccacacgctc gaccacagcc gctcggccca cggtcgctcg
     5701 cgctccgtgc gctcgcgcca gcggccgaag aacagcgcga gccagttctg caagcgcagc
     5761 gacagcgggt agggggccca gcccaccgcg cgccggccgg gcgggtggtg ggcgatccac
     5821 gaatcgacgg cgtggcgcgc gtcgtcgaag tcgagcgccc acaaccactc gaagtagtgc
     5881 aggttgtacc gccacagcaa cgattggtcg tcacagcgcc agtcgggcgg ccagcctgcg
     5941 tcgaccggtt cgttgatgaa ctcgaagcgg ccgccgagca gccgctgtgc gtcgttgcgc
     6001 tggcggcccg gggccagcgg ccggtgggcg agatgccagg cgcagcccgg atagcgcggc
     6061 cgctcccgcc gtgccaggcg ttgggggtcg ccgagccgcg cccccgagcg ccgccacagc
     6121 tgggccaaca gctggcgcgc gcgcaagtgg cgcgcggtgt gccacaacag ggccaccgac
     6181 gcttcttgcg atccgcgtct caccccacct caccggcgat cgacgcgatc tcgacgatcg
     6241 ctcgttcgcg cgcggcgcgc accgcggcga tgctcgccaa cgagacgttg agcaacccgt
     6301 cgaacggcat cagcggctcg ccgccgtggc gcacgcgatc gacgaatgcg gccaactccg
     6361 cacggtggcc cttgtcctgt cgcgccgtgc ggaaacgtcg cagcgagcgg tagccgaagc
     6421 cccgggtgac gcgaaaattc tcgacgcgca acgcacgtcc gtcggcgaag cactcgagcg
     6481 tctccttggg gtagccccgc ggtccgttgg cgaagtaatg gacggtgccc agcgacccgt
     6541 cggcgaagcc gaggacgatc gtcatcttgt cctcgcgcac ggcgggaccc gggccgacca
     6601 tctgcgcgcc gacggtgcgg atctcgcagc ccgccaagta gctgagcaga tcgatgaaat
     6661 ggcaggcctc gccgatgatc cgacctccgc cgcgcgcggc gtcctggatc cagtggtcgg
     6721 acgggatctc gccggcgttg acggtgatgc tcatgcacag cgggccgctg cgcgcggcga
     6781 gcagggcgcg ggccttctgc gtgtgcgcgc tgaaccgccg gttgtagccg accgcgagct
     6841 gcagcccggg cgccgcggcc accgcgcgcg tcagctcgcc gagctgggcg acatcgagcg
     6901 cgagcggctt ttcgacgaag acgtgcttgc cggcggccag cgcctcgcag gcgaagcggg
     6961 cgtgctggtc gtgtccgaca acgatgaaca ccgcgttcac ccgtcggtcg tcgagtacca
     7021 gccggtggtc cgacaccgcc tgtcccgcgc cgaacttcgc ggcggcgtga cgggccgaag
     7081 ccggcgtgcg gctggcgacg taggccaact cggccgaggt cgcggcgagc gccgggatca
     7141 acacgccctt ggcgaagttc ccggcgccga tcactccggc gacgacgccg ttcaccgccg
     7201 agctcggtct gaccgtgccg gcgaccgacg gcgtccgctt gacctcgggc gaatagcgca
     7261 gcacgacgcc gagcgtgttc gcgtcgccgc ggatcttgtc gtaggcctcg atcgctcggt
     7321 cgaacggcag gcgatcggtg atcagcgggt cgacccgcaa cgttccgttg tgcatcgcgg
     7381 cgagcacggc gccgaagttc cgctgctcgg tccagcggac gaagccgtag gggtagtcct
     7441 gcccgagctg ctcgtaccgt tcatcgtagc gcccaggacc gtacgagcag gagacctgga
     7501 aggtcagttc cttctcgaag aagtcgctgc gccggagatc cagcccgacg acgccgacga
     7561 gcacgatccg accgcgcttg cggcaaccct gggccgcctg atgcacgatc tcgtccgtct
     7621 ccgccgcggc cgtgatcagc acgccgtcga ctccccgatc ggcggtccac gccttcgccg
     7681 ccgcgatcgg atcaccgccg gccgacaaat cgacggtccg cgcgccgagc cgctccgcca
     7741 gctgcagccg cgcggtgtcc ggatcgacgg ccagcacctg acagccgctg gcgcgcagca
     7801 gctggacggt cagcaggccg agcaggccga cgccgaacac ggcgaaccgc tcgccgaagg
     7861 tcggatcggc cagacggatc ccctgcaggg cgatcgcgga caacacggtg aacgcggcgt
     7921 gttcgtcggt cacgccgtcc gggatgcggg cgcacaggtt gcgcggcaca cagacgagct
     7981 gcgcgtgcgg gccgttgctg gccacccgat cgcccgggct caagtcgtgc acgtcgcgac
     8041 ccgtctcgag cacgaccccg gcgttgcaat agccgagcgg aagcggctcg tcgagcttgc
     8101 ggaacaccgc ctcgagcgtc ggcagcaggc cgtcggtccg catcttgtcc agcacctggc
     8161 gcacgcggtc gggctgctgg cgggctttct gcaacaggct cgcccggctg aactcgacca
     8221 gcatccgctc ggttcccgcc gagatgaccg acgctcgggt ctggatcagt acggacccgg
     8281 agcgcagctg cggatccggt agctcggcga gctcgagtcg tccgctgcga aggtgctgga
     8341 ggatctggat catcgcgatt cctcgtccgt gcgggcggtc gcgtccggcg tccggcccac
     8401 gggcgccgct aatttgccac gttccatcgt cgagatcacg aaccgcagct tttcgcgcgc
     8461 ggtccgtccg agggagtcgg gatagacgat gtcgaaatcg agctggtccc ccactcgctt
     8521 gaggatctcg ccgcgcagcc gccgctcgtc cggctcgccc caaccgggac cgcgcaccac
     8581 ggcgacgacg atgcggccgg gctcgctttg gcggatctgc gcctcgcgta cgcgctcgag
     8641 gtccttgaag atgtgatcga gccgccccag cagcgcgccg ctcttggtca cgacgtagtc
     8701 ctcgagccgg ccgtcgagcc gcgcgaccgt ccgacccggt cggccgcagc cgcactccac
     8761 ctcgtcgagc gtggcgtggt cgccgacctc gtaacggatc aacgggaacg cggggttggt
     8821 gaaattggtc ccgacgacac gcagccgccc ggcgtcgtcc gggacgaact cggtcgccgc
     8881 gaagtcctcg tcgacgtgca accggcgctc gggacacatc gacacgttgg ccaccgcctc
     8941 ggccatgccg tagtgctcga tcggttcgac gccgaacgca cgctcgatca cccgcgcctg
     9001 cgacggcagg aggttctcgg cgccgagcgt gacccagcga accggtacgc gtatgccgaa
     9061 ctcgaggctg aacgacgcga gtagcgcgac catcgacggg taaccgtgga tccagggcgc
     9121 cccgctgtca cgcatctcgc gcagatagtc gccggcggtg tcggagttga gatggtaggc
     9181 gctgaacaac gtttggctgc cggcgtggtt gcgccgccag tagggtggtc gccgctgccg
     9241 gactggaacg atcgaccggc cacccatgta caggcacgcc gtgcccggcc gcagtccgtg
     9301 ccacccacgg tagcgccacc agaccgccca ctgctcgcga tgcgcgctcc aggtcgtgac
     9361 gaagcggagt ccggcaccgg tcgaaccgct ggtgtgcgcc gccagcagtc gacgccggcc
     9421 gatcgccggc gagcggaagc gctccggcgc ccgttggact tcttccttgg tgagcaccgg
     9481 caggaccgac agttcttcca acccgccgat ctcgcgcgga tcgatccgca gtcgctcgaa
     9541 caactcgcga taatgcggga cgtcgcgcgc ggcgcgttgg acgaactcgg cgaggcggcg
     9601 gtcccgcagc cggctgaccc gcagccggtc ccatcccgta cgctcgtcgt aggccgccca
     9661 cagctggcga aactcgcggt cgaaccgccg acggcggata cgccagcccg tcgcggtgac
     9721 accgaggttc tgcaacggga tcggcagcgc gagatacagc ttctcgagat ccatcggtca
     9781 cgcgctcgat cggagcacgg tccagccgaa atgcggccgg aagccgcccg gcaccgcggg
     9841 ctcgacgcga tcgagcgcga cgtccgcgcc gatccgttcg tcgcgcacgc gcaaggactc
     9901 catcggtttg gcggcgatcg gccagcggtg ggtttcgaac gaacccgcga cgatcggcag
     9961 cgagcgcgga tcgaagccca tcagccggca ggcgaccgca tcgatcgccg ccgggtcgtc
    10021 gccgccgatc agtacgcgcg agtcgaccgg gtctggacaa agcgggccgt tcccctcgcc
    10081 gccgacgatg ccatcgacga tcgcgacgta cggcttggcc tgtccgcgcg agcgccagct
    10141 gccgtcgcga ttgccgtaca gcagcgcgcg gttgaggtcg agcgccatcc gccagcagct
    10201 gtcgttgccg tgccagttgc cgttgcgcac cacctcgtgg ctgtcgccga gcacctgctt
    10261 gccgacgttg cgcgccttgc gatagagcca agtgccgacg cccggcaggt tgagcgccag
    10321 cctacggccg gccttcttca actcgcgctc gaccgtgtgg accaccgcgc ccgacggaaa
    10381 ctcgtcgccc ccgtcgcgcg ggctgccctc ggtgtggtgc ggcagccagt tcttgtcgcc
    10441 gttgatgccg accaggttct tcaggcagca ggtgagcccc gtcttcttgt gcgtcttcaa
    10501 cttgggcaga ttgacgaaca ggtcgcaggc cagtggcgaa ccggcgagca ggtactcctg
    10561 gatctctccc cgatgatgct cgttgaccac cgccgaatcg taatcggcgc cgtagaagcg
    10621 tccctcgccg cggtgccggt agaacagact gtcgcgcccc aggttcagcc ggacgtagcc
    10681 gcggggatcc tccggattcg gtcgccgctc gaccacgacc tgttccttgc gggcccaggt
    10741 ctcgcgccgc aggtcgatca gctcgactcg cagctgcggc cagcgcgaac ggatggagtc
    10801 gagctccgcc ggcaggtttc cgcgcgcgac gatcgccaga aagtcggcgt aggtgtgcgg
    10861 tgcgtcgcac aagcagatcg tgccgatccc gtcgagccgc tcggcgacga cctcgaccgc
    10921 ggcgacgacc aaccgcgggt tggtgatcac gggctcccag acccccggcc ggtcttcgtg
    10981 actctgctgg atccagttcg gtttgacgac gaccagccgt cgcggatccg ctcgcgagaa
    11041 gagcgcgcgc gtgccagggg cgccgtcgat cgtctcggcg atgaaacgac gcaactcggc
    11101 ggttcggtcg tagccgccga ggggtcgcag ccggacgtcg ctcatcttct ccgctcccgc
    11161 ccgcggttca tccgccacgc agactctcga ccagctcggc gacccgctcg cggaaccgag
    11221 ccaccgcgaa tcgctcgaca ccggggacgg cccgttcctg cgagcgcccc tcggccagcg
    11281 cctgcgcgat cgcctcgagc agctcgccgg gcgagtccgg gtcgaccaac ctgccgagct
    11341 cgccgtgcgc caacgcgtcg acgctcccgt cccggttgcc cgcgatgacg gggcagccgc
    11401 aggccatcgc ctcgaggaag acgatgccga acccctcgcc ggtgctgggc atgacgaaga
    11461 cgtcggccaa ccgatagtgc tccggcagct cctcgtccgg aacgaagccc gcgaagcgca
    11521 cgagagccga cacgccgcac gagtcgacca gacgctcgag ccgcgcgcga tcgtcgccgc
    11581 tgccggcgat gacgtagagc accggaccga cgcgctcggc cagccgcccg agcagcggaa
    11641 tcacacgatc gtgacccttg tagcgctctc cggccgacaa ccggccgacg gtcaacatca
    11701 ccttcctgcc gcgcgcgccg tagcgcgcga ccagccgttc gggtttcggt cccgggcggt
    11761 aacgtgcgag gtcgatcgca ttgtgcagca cgcggacacg ggcggtcggg atgccgctcc
    11821 attcgcacaa ccgccggcgc gtgtactcgc tgacggtcgt caccagatcg gaccggcgca
    11881 accccaggtt gtcgagccaa ccgcccggct gccagacatc gatcccgtgg ctcgtggtcc
    11941 acagccgcgc ccgaccgatc gccttcagcg ccagcgccag cgggccgaag ccgacggtgc
    12001 cgttgaggat cagctcgggg cgggcgcgcc gcgccagcac gagcgccgcg cgggcgaagc
    12061 ccgtcttgct tcccggcgcc ggcacgcgca cgtcgactgc cgccggtgcg ccgtccgtcg
    12121 cgccgagcag cgccagcacc gacagctcga cagccggccg gtcggcgacc gcctcgatca
    12181 ggttccgatt gaactgggcg atgccgccgc tgcggcccca ggcctcggcg acgacagcca
    12241 tgatccgaaa cggtctcacc ctcgaccccg cgcggcccgt cgatcgaagc ccgcgagcga
    12301 agcatacgga ccgcagacga tcgaggcaac gccgccgcca tcaaccggcc gcgtcgcgag
    12361 gcgcgcacgc atcggccaac agtgcgcgcg tcgcgtcgac ggccagcgaa ctgtcgaggt
    12421 aggcggccgc caagcgccgg gccgccgccg aacgcgcggc gtaggttgcg gcgtccatcg
    12481 cgacgcactc ggccaggacc cgtgcgaacg cgtcggcgcg atcgagcggc aggtcccaac
    12541 ccgcgtgccg cgcttcgagg ccgcgccaga aggtctggtc gctggtgatc accggcaggc
    12601 cggcggccag cgcttcgaga atcgcgtgac cgaagttctc gttgagcgtc gggaacagga
    12661 agaactggga cgaggagagt gccgttcgga cgcgctccgg tggcacgggc cccaacaacc
    12721 gtacctcgac cccggcgggc aacccgtcga tcaagcctcg gcaccgctcg acataggccg
    12781 catcctcgag cggcccgtag acgtcgaacc gcacgtcgcc ctcgacccgg ccgagcagct
    12841 cgagcgcgaa gtgcaggttc ttcttgcgtg cgatccggct gaggaagacg aatcgcgcct
    12901 cgcccggcct ttttcccggc agcgccgcgc cgtccgtcgg ccgtgcggtg gccggcacgt
    12961 tggggacgac acgcgccccg ccgccggtat gggtcgcgat ctcgtcgcgc tcggcgtcgg
    13021 acgaggcttg ccagatcacg ccgcggtaca gcccggtcca gcgggcgaat gcgaggaagg
    13081 cccgcttctt caccggcttg agcgtcaacg ccccggccga cagctcgccg cggggggcga
    13141 gcaccaccgg cacgcgtcgc agtctgccga gccggcgcaa cagcagcgcg cggacggcga
    13201 acgccgagaa gaagctgttg aggtagatcg cgtcgtaacg cacctcggag gcgacgcgcg
    13261 ccagctcggc gggccccgcg acgtagcacg cgcgaaagcc gtcgcgctcg agccagcggc
    13321 cagcgggcag accgttgaac ggggcgtcgt cg
//
LOCUS       DASPGN010000300         2417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_170968, whole genome shotgun sequence.
ACCESSION   DASPGN010000300 DASPGN010000000
VERSION     DASPGN010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170968"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VD788_08740"
     CDS             complement(<1..864)
                     /locus_tag="VD788_08740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV76394.1"
                     /translation="MSGFARCSAAVAAVVVASLAAVPTVRGELLEEIVANVNGDIITL
                     TDLENEEQQMLAEAYRRFTGEELDRSVRTMKARLLVEMIDRRILLDRALAMFSNVEDV
                     KNMYYEGFKKSQNVEDDAEFARMLEKDGLTVDDFKQRLLEIYAPEEVLRVEVGNRIAV
                     SSAEVERYYEEHPADFAIEDQVTVREIVLLVDDEGERAEKLAEAEAIVARVRGGGSFA
                     EAAEAHSDAGTKTAGGLLGTVKRGHLAEALEEVAFELAAGDVSAPIETPYGYHILLVE
                     SRTVGEKSQLDA"
     gene            complement(926..1888)
                     /locus_tag="VD788_08745"
     CDS             complement(926..1888)
                     /locus_tag="VD788_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV76395.1"
                     /translation="MTAVGRKVALWAIASAVVVGCDDPPSDPAIGVAAYVEGSPVYVD
                     EVEHYLDSNLVMDETMEDLAPGILDEIKSRLFDAMLEERMLLAEAGRRGIEVGPLEVT
                     AYLELETSIDDEEDTDSRHLRQTEARQRLMVQKLQETIIRERPPLTDDEVATYAVDHR
                     KTLLPAEPVELRALQLGSLKQADRIYKEIRRKRMTFNEATLAYERSPGQALPTRMSWE
                     SLPDELRATLEELKPGQVSKPLELHGEIYLFHVGKWLSDPADHDAELLLRARSRLESE
                     RNRNSLDALMDDLRNRANIRIKKSNLSFDYVPAEGDPIARWVDS"
     gene            complement(1885..>2417)
                     /locus_tag="VD788_08750"
     CDS             complement(1885..>2417)
                     /locus_tag="VD788_08750"
                     /inference="COORDINATES: protein motif:HMM:NF015426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HXV76396.1"
                     /translation="RQHGHIAAVGFDLYCQILERAVGELKGEAVEEPHPVSLHLGVDI
                     KIPESYLPEAGDRLVIYKRLAGARDDDDIDRLQAETEDRFGRLPVAGRNLFAMGRLRV
                     LAEAAGVKAIDLIDDRLQIRFHDLPAVDPRTVLAVTAEQGGTLKPSGVVVLPAPPQGA
                     DRIGSVAALLDRMLPS"
BASE COUNT          356 a          853 c          771 g          437 t
ORIGIN      
        1 ggcgtcgagc tgcgactttt ctcccacggt gcgcgactcg acgagcagga tgtggtagcc
       61 gtagggcgtc tcgatcggcg cggagacgtc ccccgcggcc aactcgaacg cgacctcctc
      121 gagcgcttcg gccaagtgcc cgcgcttcac cgtccccaag agaccgcccg cggtcttggt
      181 tcccgcgtcc gagtgggcct ctgccgcctc ggcgaacgac ccgccgcccc gcacgcgggc
      241 gacgatcgcc tcggcctcgg ccagcttttc cgcccgctcg ccctcgtcgt ccaccagcag
      301 gacgatctca cgcacggtga cctggtcctc gatcgcgaag tccgccgggt gttcttcgta
      361 gtatcgctcg acttccgccg agctcaccgc gatgcggttg cccacctcga cccgaagcac
      421 ttcttccggc gcgtagatct cgagcagacg ctgcttgaag tcgtcgaccg tcagcccgtc
      481 cttctcgagc atgcgggcga actcggcgtc gtcttcgacg ttttggctct tcttgaagcc
      541 ctcgtagtac atgttcttga cgtcttcgac gttggagaac atcgccagcg cccgatcgag
      601 caggatccgg cggtcgatca tctcgaccag cagccgcgcc ttcatcgtgc gcacgctgcg
      661 gtcgagctct tcgccggtga agcgccggta ggcctcggcg agcatctgtt gctcttcgtt
      721 ctcgagatcg gtcaaggtga tgatgtcacc gttgacgttg gccacgatct cttccaggag
      781 ctcgccgcgc acggtcggta cggcggcgag cgacgccacg acgaccgccg cgaccgccgc
      841 gctgcatcga gcgaagccgg acatcgatcg gacctccgag ttggcccccg gacagtgtag
      901 tcaacgcccc gcgccgcggc caacgtcagg aatcgaccca ccgggcgatc gggtcccctt
      961 cggccggaac gtaatcgaac gacaggttgc tcttctttat gcggatattc gcgcgattgc
     1021 gcaagtcgtc catcagtgcg tccagagagt tgcggttgcg ctcgctttcc agccggcttc
     1081 gggcccgcag cagcagctcg gcgtcgtggt cggcagggtc ggacagccac tttcccacgt
     1141 ggaagaggta gatctccccg tgcagctcga gcggcttgct gacctggccc ggcttgagct
     1201 cctcgagcgt ggcccgcagc tcgtcgggca gactctccca cgacatgcgc gtcggcagcg
     1261 cctggccggg tgatcgctcg taggccagcg tcgcctcgtt gaacgtcatc cgtttgcggc
     1321 gaatctcctt gtagatccga tcggcctgct tgagcgagcc cagttgcagc gcgcgcagct
     1381 cgaccggctc ggccggcaac agcgtcttcc ggtggtcgac ggcataggtc gccacctcgt
     1441 cgtcggtcag cggcggacgc tcgcggatga tcgtctcctg gagcttctgg accatcaacc
     1501 gctgacgcgc ctcggtctgg cgcaggtgtc gcgagtcggt gtcttcctcg tcatcgatcg
     1561 acgtctcgag ctccaggtat gccgtgacct cgagcgggcc gacttcgatc ccgcggcgcc
     1621 cggcttcggc gagcagcatt ctttcctcga gcatcgcgtc gaacaaccgg ctcttgatct
     1681 cgtcgaggat cccgggggcg agatcctcca tcgtctcgtc catgacgagg ttcgagtcga
     1741 gatagtgctc cacctcgtcg acgtagaccg ggctcccctc gacgtaggca gccacgccga
     1801 tggcggggtc gctcggcggg tcgtcgcagc cgacgacgac cgccgacgcg atcgcccaca
     1861 gcgcgacctt acgcccgacg gcggtcacga cggaagcatc ctgtcgagca aggcagcgac
     1921 cgaaccgatc cggtcggcgc cctgcggtgg ggccggaagt accacgacac ccgatggttt
     1981 gagcgtgccg ccctgctccg cggtgaccgc gagcaccgtg cgcgggtcga ccgcggggag
     2041 atcgtggaag cgaatctgca gccgatcgtc gatcaagtcg atcgccttca cccccgcggc
     2101 ctcggccagg acgcgcagcc ggcccatggc gaacaggttg cggccggcga cggggagtct
     2161 gccgaagcgg tcctcggtct cggcctgcaa ccggtcgatg tcatcgtcgt cccgggcgcc
     2221 agccagccgc ttgtaaatga ccagccggtc ccctgcctcg ggaaggtagc tctccgggat
     2281 cttgatgtcg acccccaggt gcaggctcac cggatggggc tcctcgaccg cctcgccctt
     2341 cagttcgccc accgcgcgct cgaggatctg gcagtacagg tcgaagccca ccgcggcgat
     2401 gtgcccgtgc tggcgcg
//
LOCUS       DASPGN010000301         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_756960, whole genome shotgun sequence.
ACCESSION   DASPGN010000301 DASPGN010000000
VERSION     DASPGN010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756960"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VD788_08755"
     CDS             complement(<1..1128)
                     /locus_tag="VD788_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76397.1"
                     /translation="MISFLRSSLLRYVAPAFLGTAAAACDPGYVEAVFLYKGDPPVLG
                     PDATTFARVERRTDPLKPGEPVTTVATSPYAQHGGPIPTIPYGDDLVIIIEVRLSPDP
                     AGQVVLYGISDRFSVRQGDHIRVPILLHPRPPPEPLPGDSSLVIVTSTRAPGYTNRRR
                     VELHLFAPESAVKARVSNLVDLPEDGSVVFDLAELQRVESATAGYPGYRVPAWTLDVE
                     SCPDTGRCRLNVYTRFTDNQEYSGPPAAASVTYDDVPPSLIPGLTSIFPPVARDDVRI
                     SVNLTASETIFSPRLAVAQRVDFPLVRVSPAEDQPGAAFTFSSTHTAGSIGRDGTFLV
                     RADLVDLAGNVARLVEVGSFTVDTIVPEIGGVTVEPRRINGE"
     gene            complement(1131..>2413)
                     /locus_tag="VD788_08760"
     CDS             complement(1131..>2413)
                     /locus_tag="VD788_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76398.1"
                     /translation="LVLLASFSARAEPVKPPVRLLVYTLSSPERWAPLAKDLSEGIVA
                     HVAGPAARKGITALSESDVALMGTFIAERADLSEEDCRKAERCLARLSQAADANKTLY
                     GWVDRVGDSYVAVLTLLDAVTGEREGTKSVISGSEGEFRTLLLETADEILGLSTSSPE
                     GGFSPEPLPAHGQKVAVVPFTGLEGEPGIAETLTNLFSVELLKYEYSVLTRSELIVLL
                     RDAMERRSLLNESRPLDEIIVPLAGAYGADYLATGHVAKFDRSYIIVLKLILISEASV
                     IRRYVEVYRGGVDYLPAALKFAAARLFGREMPGAGDLSILTDVEGEYRIDRGRPGALP
                     QATPHVGLAATKHELSISAPGYLPYSADLYIDGESAEPLKYRPALEPLAAEWYESPIV
                     WGIIGVAVAGATTAILLATVPPNGADSSFQGERE"
BASE COUNT          436 a          807 c          789 g          381 t
ORIGIN      
        1 ttcaccgttg atgcgccgcg gctcgacggt cacaccgcca atctccggga cgatcgtgtc
       61 gaccgtgaac gagccgacct ccacgagccg cgcgacgttc ccggccagat caacgaggtc
      121 ggcacgcacg aggaacgtcc catcgcgtcc gatcgatccg gccgtgtgcg tcgacgaaaa
      181 cgtgaacgcc gcgcccggct gatcctcggc cggcgacacg cggaccagtg ggaagtcgac
      241 ccgctgcgcg accgcgaggc gcggtgagaa gatggtctcg gaggccgtca aattgaccga
      301 gatgcgcacg tcgtcgcgag cgaccggcgg aaaaatgctc gtgaggcccg ggatcaggct
      361 cggcgggacg tcgtcgtagg tgacggaggc ggccgcgggc ggcccggaat attcctggtt
      421 gtcggtgaag cgtgtgtaaa cgttgaggcg gcaccgcccg gtatccgggc acgactcgac
      481 gtcgagcgtc catgccggca cccgatagcc tggatatccg gcggtcgcgc tctcgacgcg
      541 ttggagctcc gccagatcga agaccaccga tccgtcttcg ggaagatcga cgaggttcga
      601 aacacgcgcc ttcacggcgc tctccggagc gaacaggtgc agctcgaccc ggcggcggtt
      661 cgtatagccg ggcgcccgcg tcgaggtcac gatcaccagg ctgctgtcgc cgggcagcgg
      721 ctccggcgga ggccgcggat ggagcagaat cgggacgcgg atgtgatcgc cctggcgcac
      781 gctgaagcga tcggagatgc cgtacagaac cacctgccca gcgggatccg gcgacagccg
      841 cacttcgatg atgatcacga ggtcatcgcc atacgggatc gtcggtatcg gccctccgtg
      901 ctgcgcatac gggctcgtgg ccacggtcgt caccggttcg cccggcttga gcggatcggt
      961 tcgccgttcg acccgcgcga aagtggtcgc gtcgggaccc aaaacgggcg ggtcgccttt
     1021 gtacaagaac accgcttcga catagcccgg gtcgcaggcc gctgcggccg tccccaggaa
     1081 ggccggagcg acatagcgca ataacgaaga ccgcaggaac gagatcatac ctattccctc
     1141 tcaccttgaa agctcgagtc cgcgccgttc ggcggcacgg tcgcgagcaa aatcgccgtc
     1201 gtcgcccccg cgaccgcaac gccaatgatc ccccacacga tcggcgactc gtaccactcg
     1261 gcggcgagcg gttcgagcgc cggccgatac ttgagcggct cggcggattc gccgtcgata
     1321 tagagatcag ccgaatacgg cagatagccg ggcgccgaga tgctgagctc gtgtttcgtc
     1381 gccgcgaggc cgacgtgcgg cgtcgcttgc ggcaaagcgc ccggccggcc gcgatcgatg
     1441 cgatattcgc cttcgacgtc ggtgaggatc gacaggtcgc cggcgccggg catctcgcgc
     1501 ccgaagagcc tcgcggccgc aaatttcagc gccgcgggca aataatcgac tccgccccgg
     1561 tatacctcga cgtaacggcg gatcacgctc gcttcgctga tcagaatgag cttgaggacg
     1621 atgatgtagc tccgatcgaa cttcgcgacg tgtcccgtcg ccagatagtc ggcgccgtac
     1681 gcgcccgcca gcggcacgat gatctcgtcg agcggccggc tctcgttcag gagcgacctc
     1741 cgctccatgg cgtcgcgcag aaggacgatc agctcggatc gagtcaaaac cgaatattcg
     1801 tacttcaaga gctcgacgct gaataagttc gtgagcgtct cggcgatccc cggctctcct
     1861 tcgagcccgg taaaaggaac gacggcgacc ttctgcccgt gggcggggag cggctccggc
     1921 gaaaagcctc cttcggggct cgacgtcgag aggccgagta tttcgtccgc cgtctcgagg
     1981 agcagcgttc gaaactcacc ctcgctcccc gaaatgacgc tcttcgtccc ctccctctcg
     2041 cccgtcacgg catcgagcag ggtcagcacg gccacgtaac tatcgccgac gcgatcgacc
     2101 cagccgtaca gcgtcttgtt cgcgtcggcc gcttgagaga gccgggcgag gcagcgctcc
     2161 gcctttcggc agtcttcttc gctcaaatcc gctctctcgg cgatgaacgt gcccatgagc
     2221 gccacgtccg actcggacag cgcggtgatt cccttccgcg ccgccggtcc cgcgacgtgg
     2281 gcgacgatcc cttcggagag gtctttcgcg agcggcgccc agcgttccgg cgaagagagc
     2341 gtgtacacga ggagacgcac cggcggcttc accggctcgg ctcgagcgga aaaagacgcg
     2401 agcaacacca ggg
//
LOCUS       DASPGN010000302         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1576810, whole genome shotgun sequence.
ACCESSION   DASPGN010000302 DASPGN010000000
VERSION     DASPGN010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576810"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VD788_08765"
     CDS             <1..852
                     /locus_tag="VD788_08765"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HXV76399.1"
                     /translation="TAIKAAGFSMRRTTMPVLLITAMLCGLLYIVEDSVAPVSNRLAQ
                     ETEDKIMGRAPRTYGPTVGGRWGLGPEGNTLYHYQLFDPQRREFQGLTVLSIDRHEPR
                     IIDHLYAQKATWLGDKAELDNGWFRSFGDGTTPGAYEVFAGVREIQLEPPAQLVTKQL
                     DSGRHSALGEQLSTRELKDEIAVLERRGYDTTRLEVAYHGKISRTMAPLVMVVLGLPF
                     AFRVGRRGSLYGIGVALLLVLVYWAVFAVFNAMGLETLLDPWAAAWAPNILFGLLGSY
                     LLLYIPT"
     gene            complement(883..>3567)
                     /locus_tag="VD788_08770"
     CDS             complement(883..>3567)
                     /locus_tag="VD788_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76400.1"
                     /translation="PDDWRRLHEAAASDGIEGAVLEAVRHAAAQLRPGSELERDQTTL
                     VQELGAGLGPALAERGLDLRRLELDSVDFLAVRDGSDPPEPTDVRLLVIGLDGLDWEI
                     VDELVEAGRLPNLGRLIERGARAKLLTISPMLSPVIWTTIATGVEPSRHGVLDFLVQD
                     ASGQSRQPVTSAQRRVPTVWELLSRAGIEVGVTAWWASWPADPVRGYLVSDRVAYQLF
                     GYRADPEDAQGKSWPPELYADRVRPRIVPPAEIDWTEVVPYLDGPRKSRAEFDDDERV
                     LLDEFRTLLAAGRTYLAIADDLRQNYRPRFEVAYFEGTDTIGHLFMPYRAPRLEGIPD
                     KRFASFSQMVDRYYEAIDGYVGQLLADRGDTWTVMVLSDHGFASDATRPRTTDSRVGH
                     GAAADWHRRFGVLILSGAQIVPGVRLEEASVFDIAPTVMALFGQPVPDGWPGRVLAGA
                     IDPSFLDRHPVRYRLDEPARSELLAEGLIDPAAADLLEKLQSLGYVSSGTRGGDSVTA
                     RNNAGVALMAEGRYEEAEREFRAGLEAAPGSPMLALNLGLAQRLQGRNDEAARLFRGA
                     LDHTITRRMAGNLLAQILLERAELDEAERIVARVLADEPDAAELRNTLGQLHERRGLT
                     NEARHEYERAASLDPNAALPRNNLGNLARRAGDLAEAERWYLAAIEADPYFMGAYNNL
                     ALVYQDRGEMERAHDLYARALDKSPGNADVLNNFGSWHYARGEFDEARKYWTRASGAD
                     PRNPSPLNNVAGLEINAQRFDEAERLLRRALELDPGYGDARMNLSIVLMARGAVDEAR
                     RQLLRACEDPRTGAGSWAKLGALELDQGNLDRAIEVLERARALAPASTEALNYLGEAY
                     RRVGRTEEAMSLWRRSLEVDPGQPGLRRYLDRGADGSE"
BASE COUNT          515 a         1281 c         1215 g          556 t
ORIGIN      
        1 accgcgatca aggcagccgg cttcagcatg cgccgaacga cgatgccggt cctgctgatc
       61 accgcgatgc tctgcggcct gctctacatc gtcgaggact cggtggcacc ggtgtcgaac
      121 cgactggcac aggagaccga ggacaagatc atgggacgcg ccccgcggac ctacggtccg
      181 accgtcggcg ggcgctgggg actcggcccc gaaggcaaca cgctctacca ctaccagctg
      241 ttcgacccgc agcggcgcga gttccaaggg ctgaccgtcc tttcgatcga ccgccacgag
      301 ccccggatca tcgaccacct ctacgcgcag aaggcgacat ggctcggcga caaggccgag
      361 ctcgacaacg gctggttccg ctcgttcggc gatggcacca cacccggggc ctacgaggtc
      421 ttcgcgggtg tacgcgagat ccagctcgag ccccccgccc agctcgtgac gaagcagctc
      481 gattcggggc gccacagcgc gcttggcgaa cagctcagca cgcgcgagct gaaggacgag
      541 atcgccgtgc tcgaacgacg gggctacgac acgacgcgcc tcgaggtcgc ctatcacggc
      601 aagatctcgc ggacgatggc accgctcgtg atggtggtgc tcggcctgcc cttcgccttc
      661 cgcgtcgggc gccgcggctc gctctacggg atcggcgtcg cgctgctgtt ggtcctggtc
      721 tattgggcgg tcttcgccgt gttcaacgcg atggggctcg agacgttgct cgacccgtgg
      781 gccgccgcct gggcccccaa catcctcttc ggcctcctcg ggtcgtacct gctgctgtac
      841 attccgacct gatcggcgcg gctccgacac gcgcggaggg gctcactcgc taccgtcggc
      901 cccgcggtcg agatagcgtc gtaagcccgg ctgcccggga tcgacctcga gtgatcggcg
      961 ccagagcgac atcgcctcct ccgtccggcc gacccggcgg taggcttcgc cgaggtagtt
     1021 gagcgcctcg gtgctggccg gcgccaacgc ccgtgcccgc tcgagcactt cgatcgcgcg
     1081 gtcgaggttg ccctggtcga gctcgagcgc gcccagcttg gcccacgagc cggctcccgt
     1141 ccgcgggtct tcgcaggctc gcagcaattg acgccgtgcc tcgtcgaccg ccccacgtgc
     1201 catcaacacg atcgacaggt tcatccgcgc gtcgccgtag ccgggatcga gctcgagcgc
     1261 cctgcgcaat aaccgctccg cctcgtcgaa cctctgggcg ttgatctcga ggccggcaac
     1321 gttgttcaac ggcgacgggt ttcgcgggtc cgcccccgag gcacgcgtcc agtacttgcg
     1381 cgcctcgtcg aactcgccac gcgcgtagtg ccagctgccg aagttgttca gcacgtcggc
     1441 gttgccgggc gatttgtcca gggcccgcgc gtagagatcg tgtgcgcgct ccatctcccc
     1501 gcggtcctgg tagaccagcg ccaggttgtt gtaggcgccc atgaagtacg ggtcggcttc
     1561 gatcgccgcc aggtaccatc gctccgcctc ggcgaggtcg cccgcgcgtc tggcgaggtt
     1621 gccgaggttg ttgcgtggca gagcggcgtt cgggtcgagg ctcgccgcgc gctcgtactc
     1681 gtgtcgtgct tcgttcgtga gaccgcggcg ctcgtggagc tgcccgagcg tgttgcgcaa
     1741 ctcggccgcg tcgggctcgt cggccagcac ccgtgcgacg atccgctccg cctcgtcgag
     1801 ctcggcgcgc tcgagcagga tctgtgcgag cagattgccc gccatccggc gggtgatcgt
     1861 atggtcgagt gccccgcgga acaagcgggc cgcctcgtcg ttgcgccctt gcaggcgctg
     1921 ggccagcccg aggttgagcg ccagcatcgg cgatccgggc gccgcttcga gacccgcgcg
     1981 gaattcccgc tcggcttcct cgtaccgccc ctcggccatc aacgccacgc cggcattgtt
     2041 gcgcgcggtg accgagtccc ctcctcgcgt tccgctcgag acgtagccca ggctctgcag
     2101 cttctcgagc agatcggcgg ccgccgggtc gatcaggcct tcggccagca gctccgaccg
     2161 ggccggctcg tcgaggcggt accggaccgg atgccgatcg aggaacgacg gatcgatcgc
     2221 cccggcaagc acgcgtccgg gccagccgtc gggcaccggc tgcccgaaca gcgccatcac
     2281 ggtcggtgcg atgtcgaaga cactcgcctc ctcgagacga acgccgggca cgatctgcgc
     2341 cccggagagg atcagcacgc cgaatcggcg gtgccagtcg gccgccgcgc cgtggccgac
     2401 ccgcgagtcg gtcgtgcgcg ggcgggtggc gtccgaggcg aagccgtggt cgctgaggac
     2461 catcaccgtc caagtgtcgc cgcggtcggc gaggagctga ccgacgtagc cgtcgatcgc
     2521 ctcgtagtag cgatcgacca tctgcgaaaa gctcgcgaac cgcttgtcgg ggatcccctc
     2581 gagtcgcggt gcccggtagg gcatgaacag gtggccgatc gtgtccgtgc cctcgaagta
     2641 ggccacctcg aagcgcggcc ggtagttctg gcgcaagtcg tcggcaatcg ccaggtaggt
     2701 gcgacccgcc gcgagcagcg tgcggaactc gtcgagcagc acccgctcgt cgtcgtcgaa
     2761 ctcggctcgg ctcttgcgcg ggccgtcgag atagggcacg acctcggtcc agtcgatctc
     2821 ggccggcggg acgatccgcg ggcgcacgcg gtcggcgtag agctcggggg gccagctctt
     2881 gccctgcgcg tcctcggggt cggcccggta gccgaacagt tggtaggcca cgcggtccga
     2941 gaccagatag ccgcgcaccg ggtccgccgg ccacgaggcc caccacgccg tgacaccgac
     3001 ctcgatgccg gcgcgggaca gcaattccca aaccgtcggc acgcggcgct gcgcggaggt
     3061 gacgggctga cgagactggc cgctcgcgtc ctggacgaga aaatcgagca cgccgtgccg
     3121 gctgggctcg acgccggtgg cgatcgtcgt ccagatcacg ggggacagca tcgggctgat
     3181 cgtgagcagc ttggctcgcg cgccgcgctc gatcaaccgc ccgaggttcg gtagccgacc
     3241 ggcctcgacg agctcgtcaa cgatctccca gtcgagcccg tcgaggccga tgacgagcag
     3301 acgcacgtcg gtcggctcgg gcggatcgct cccgtctcgc accgccagga agtcgaccga
     3361 gtcgagctcg agccgccgca gatcgagacc gcgctcggca agcgccggtc ccaggccggc
     3421 acccagctcc tgcaccagcg tcgtctggtc ccgctcgagc tcgctgccgg gacgcagctg
     3481 cgctgcggca tgccgtaccg cttcgagcac ggccccctcg atcccgtcgc tcgcggcggc
     3541 ctcgtgcaac cgccgccaat cgtcggg
//
LOCUS       DASPGN010000303         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1576866, whole genome shotgun sequence.
ACCESSION   DASPGN010000303 DASPGN010000000
VERSION     DASPGN010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576866"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VD788_08775"
     CDS             <1..548
                     /locus_tag="VD788_08775"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HXV76401.1"
                     /translation="ERVSYETRLEGDVPVYRAVLEADVVLEPGRRDPAFQLELASEPD
                     SGTLRAGESLRLTVTPSKDCYVTVLHLDPQGGVERVFPNRFQPDHGVRGGTARTIPAP
                     EDGFELRAELERGRHRQHEQILVVATLDPIRFEVATASDPDELVPNAIESIALSRLNR
                     WLLEVPVDRRVEALWSYEIVE"
     gene            545..2662
                     /locus_tag="VD788_08780"
     CDS             545..2662
                     /locus_tag="VD788_08780"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HXV76402.1"
                     /translation="MRAADRRTVRLLALAAIVGSSPWPPASHGTAPYTTRTEIADPWS
                     AAPPSDRWPRRIDWHDPSLELHLESIERHGERTRLWLALVLKPAVEEAGTSIGPARYR
                     TTSGRWQQTPIHGLPDGARALTLSRSDTPRRELWLEVASEVATLRITLGDDTLDLELT
                     GEPGGLASATPSAAATAPAALAPGRPVRLETVVQLEHRPFRDGALDPGETDELEIEVR
                     NLGSRVAEGVTVAAEASAPGVELPDAIDVPPIPPGSSTTVRLPVTADADLADGTLHLT
                     IRTREPFGHDASPVELVIPTRAHPPAQLVLTDDFAIEGHAVPLPRDSVVTLRLRVRNV
                     GQGSAADVRASVTPGDGVYPAGDTARLFELGSLGPGEVAELSFRCYANQHASQLELHV
                     DLSHRENRDAPARSLLALPLAGASSVARIVRLEAAPRTATVLEPAPPPLVSEVDRFVP
                     ASRSSRPAALAVVIGVERYASAPGATFAAADARTASRYFELVLGIPAERIQLLVDEQV
                     TLGQLHRVFGPGGWVARRMTDASELFVFFAGHGVATGSGFAPYLIPVDGDLNYVEQTG
                     FALDRLIAWLSELGARSTTVFLDTCFSGLTREGAALFATARPLVIVPRPTHPSGLSLF
                     SAARGTQLASAHEAEGHGLFSYVLFKGLSGAADSDGDGRIVAGELEGYVEREVARTAM
                     ELDREQDPGVFVADEAAVVVEFR"
     gene            complement(2670..>3387)
                     /locus_tag="VD788_08785"
     CDS             complement(2670..>3387)
                     /locus_tag="VD788_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXV76403.1"
                     /translation="IGRRIRAALAGHEQSVIEGLQFVGGKALSAGAGVVRGAWALVGW
                     FVVPVYFVFFLIGGGVQTGSLSDYLPFLKPETRRDVVFLVGEFANILVAFFRGQLIVA
                     VLQGLLFALGFSVVGLKFGFVIGLVLGFLNVIPYLGSMVGLGVALPLAYFQQGGGWSA
                     LALVAAVFVVVQVIEGYLLTPRIMGQRTGLHPIAIIFAVFFWGSALDGILGMILAIPL
                     TAFLVVFWRLAKEKYIAEWV"
BASE COUNT          516 a         1246 c         1158 g          467 t
ORIGIN      
        1 gggaacgcgt ctcgtacgag acgcggctcg aaggcgacgt gccggtctac cgcgcggtgc
       61 tcgaggccga cgtcgtcctc gagccgggcc gccgtgatcc ggcattccag ctcgagctcg
      121 cgagcgagcc ggacagcggc acgctgcgtg ccggcgagtc cctgcggctg accgtcacgc
      181 cgtcgaagga ctgctacgtc accgtgctcc atctcgatcc gcagggcggg gtcgagcgcg
      241 tcttccccaa ccgattccaa ccggatcacg gcgtgcgagg cggaaccgcg aggacgatcc
      301 cggcgccaga ggacgggttc gagctccggg ccgagctcga gcgcggacgg caccggcagc
      361 acgaacagat cctggtcgtc gcgacgctcg accccatccg gttcgaggtc gcgaccgcaa
      421 gcgacccgga cgaattggtg ccgaacgcga tcgaatcgat cgcgctcagc cgtctcaacc
      481 gctggctgct cgaggtcccc gtcgatcgac gggtcgaggc gctgtggtcc tacgagatcg
      541 tcgaatgagg gcggcggacc ggcggacggt gcggctcctc gcgctggcgg cgatcgtcgg
      601 gtcgtcgccg tggccgccgg cctcgcacgg cacggcaccg tacacgacgc gaacggagat
      661 cgccgatccg tggagcgccg ctccaccgtc ggaccgctgg ccgcgacgga tcgactggca
      721 cgacccgtca ctcgagctcc atctcgaatc gatcgagcgc cacggcgagc ggacacggct
      781 ctggctcgcg ctcgtgctca agcccgccgt cgaggaagcc ggaacctcga tcggcccggc
      841 ccgctatcga acgacgtcgg gccgctggca gcagacgccg atccacggat tgccggacgg
      901 agcacgggcg ctgaccctgt cgcgaagcga cacgccgcgc cgcgagctgt ggctcgaggt
      961 ggcgagcgag gttgcgacgc tgcgcatcac gctcggcgac gacacgctcg acctcgagct
     1021 gaccggcgag cccggcgggc tcgcgagcgc aacgccgtcc gcggccgcga ccgcgccggc
     1081 cgccctcgcg ccggggcgac ccgtacggct cgagacggtg gtgcagctcg agcaccgccc
     1141 gttccgggac ggcgcgctcg acccgggcga gaccgacgag ctcgagatcg aggtgcgcaa
     1201 tctcggctcg cgcgtcgccg agggtgtcac cgtcgcggcc gaggccagcg cgcccggggt
     1261 cgagctgccg gacgcgatcg acgtcccgcc gattccgccc ggctcgagca cgaccgttcg
     1321 cctgccggtc acggccgatg cggatctggc ggacgggacc ctccacctga cgatccgcac
     1381 tcgcgagccg ttcggtcacg acgcgtcgcc ggtcgagctc gtcatcccga cgcgcgcgca
     1441 cccgcccgcg cagctggtgc tgaccgacga tttcgccatt gagggccacg ccgttccgct
     1501 cccgcgcgat tcggtggtca cgctccggct gcgggtgcgc aacgtcggtc aggggtccgc
     1561 cgcggacgtc cgcgcgtcgg tcacgccggg tgacggggtc tatccggcgg gcgacaccgc
     1621 ccgcctgttc gagctcggct cgctcggtcc gggcgaggtc gccgagctga gctttcgttg
     1681 ctacgccaac cagcacgcct cacagctcga gctccacgtc gacctgtccc accgcgaaaa
     1741 ccgcgacgcg ccggcgcgat cgctgctcgc gctcccgctc gccggtgctt cgtcggtcgc
     1801 gcggatcgtc cgcttggagg ccgcgccccg gaccgcgacc gtgctcgagc cggcgccgcc
     1861 gccgctggtt tccgaggtcg accggttcgt tcccgcctcg cgcagctcgc gccccgcggc
     1921 gctcgccgtc gtgatcggcg tcgaacgcta cgcctccgca cccggcgcca ccttcgccgc
     1981 cgccgacgca cggaccgcca gccgctactt cgagctcgtg ctgggcattc ccgccgagcg
     2041 catccagttg ctggtcgacg agcaggtgac gctcgggcag ctgcaccgcg tgttcggccc
     2101 cggcggttgg gtcgcccggc ggatgaccga cgcgagcgag ctgttcgtct tcttcgccgg
     2161 acacggagtg gcgacgggct ccggcttcgc cccctatctg atcccggtgg atggcgacct
     2221 gaactacgtc gagcagaccg ggttcgcgct cgaccgcctg atcgcgtggc tgtcggagct
     2281 cggcgctcga tcgacgacgg tgttcctcga cacctgcttc agcgggttga cccgcgaggg
     2341 ggccgcactg ttcgcgaccg ctcgaccgct ggtcatcgtg ccgcggccga cccacccctc
     2401 ggggctgtcg ttgttctccg ccgcccgcgg cacgcagctc gcctcggccc acgaggccga
     2461 aggacacggg ctgttcagct acgtgctgtt caaagggttg tcgggcgcgg ccgattccga
     2521 cggcgacggt cgtatcgtcg ccggtgagct tgagggctac gtcgagcgcg aggtcgcgcg
     2581 aaccgcgatg gagctcgacc gcgagcagga ccccggcgtg ttcgtcgccg acgaggcggc
     2641 ggtcgtcgtc gagtttcgct gacgggcggt cagacccact cggcgatgta cttctccttc
     2701 gccagcctcc agaagacgac gaggaacgcg gtcagtggga tggcgaggat cattccgagg
     2761 atgccatcca gcgccgagcc ccagaagaag acggcgaaga tgatcgcgat cggatggagt
     2821 cccgtgcgct ggcccatgat ccgcggcgtg agcaggtatc cttcgatcac ctgcaccacg
     2881 acgaacaccg cggccaccag cgccagcgcc gaccaccctc ccccctgctg gaagtaggcc
     2941 agtggcagcg cgaccccgag accgaccatg ctgcccaggt acgggatgac gttgaggaaa
     3001 ccgagcacca agccgatcac gaagccgaac ttcagcccga ccacgctgaa accgagggcg
     3061 aacagcagac cttgcagcac ggccacgatc agctgtccgc ggaagaacgc gaccaggatg
     3121 ttggcgaact cgccgaccag gaagacgacg tcccgccggg tctcgggttt gaggaacggc
     3181 aggtagtcgg acaacgagcc ggtctgcacg ccgccaccga tcaggaagaa gacgaagtac
     3241 accggcacga cgaaccaccc gaccagcgcc caggcgccac gcaccacacc ggcgcccgcc
     3301 gacagcgcct tgccgccgac gaattgcagc ccctcgatca cactctgctc atgacccgcg
     3361 agcgccgcgc ggatgcgacg cccgatc
//
LOCUS       DASPGN010000304         3398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_874665, whole genome shotgun sequence.
ACCESSION   DASPGN010000304 DASPGN010000000
VERSION     DASPGN010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874665"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..600)
                     /locus_tag="VD788_08790"
     CDS             complement(61..600)
                     /locus_tag="VD788_08790"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXV76404.1"
                     /translation="MSPDPHPAGINIHYLDQGSELIENLTDELYAAGTGERSRGGVGA
                     QLRHCLDFYDCFLAGITERAIDYNRRKRDTRAETDRSHAAERLHKVRETLLRLTADTG
                     TRPLRVRAECGAAGAAEWSDSSVARELQFLVSHTIHHYALIAMMLRTQGYDVAREQPD
                     FGIAPSTLAHWKEIGSFVG"
     gene            complement(597..1418)
                     /locus_tag="VD788_08795"
     CDS             complement(597..1418)
                     /locus_tag="VD788_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV76405.1"
                     /translation="MSRLRGVAKLLALVVANAVFFGVIVVSSVGLVWLARFGRTGRGD
                     PAWRTGVFHAWGKTMARLLGMRVTTGGNHPRPPFVLVSNHLGYVDVVLLASELRCVFV
                     SKAEVRDWPVVGRLCSSVDTLFIRREQKREIPRVMTEMERVLASGRGIVLFPEGTSSP
                     GDDVLRFRPSLLESAARTGRPVHYACLAYRTPAGSPAARDAVCWWGKMPFVSHVFGLL
                     SLPGFEARIEFGDQPIVERERKALTQRLQREVERQFRTVIWVRSAERDRARKENR"
     gene            complement(1429..2238)
                     /locus_tag="VD788_08800"
     CDS             complement(1429..2238)
                     /locus_tag="VD788_08800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HXV76406.1"
                     /translation="MSSSKGASVADYPVEPQSIPELKLAKAGYSVRFATSPAELKSVQ
                     QLRFEVFNLELGEGLEESYKTGRDQDRFDAYCHHLMVLDANDEVIGTYRMQTDEMAAN
                     HIGYYSADEFDLSRMPRNLLDNSVEVGRACVAKPFRNRYVLFLLWCGLAAYMTHNRKR
                     YLFGCCSLTSQDPDEGRRVMDYLRAAGHVRSDFRIEPRPDWRCYDAGEPPASTLPEGK
                     IELPKLFSLYLRYGAQVCGPPAIDRCFKTIDYLVVLDVERLGADARKTFFA"
     gene            2381..3049
                     /locus_tag="VD788_08805"
     CDS             2381..3049
                     /locus_tag="VD788_08805"
                     /inference="COORDINATES: protein motif:HMM:NF013378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HXV76407.1"
                     /translation="MEVIPLPPADDYPSVEDGPEVEHRVRGSRFLGRAFHVRRASEAA
                     VRVREVRRAHHAARHHAWASRIGPPNDVVERADDDGEPSGTAGRPILQCIGGRSLSDV
                     LLVVTRYFGGTKLGTGGLARAYADAARLALDGCVGRVVEIDCTIEIVLAYADLGFLEA
                     ALARSIDGIRRVSREYDPSPRIRLAVRPTRAAGIAASIIDATGGRASVSLVRSALARG
                     PGAV"
     gene            3106..>3398
                     /locus_tag="VD788_08810"
     CDS             3106..>3398
                     /locus_tag="VD788_08810"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76408.1"
                     /translation="MSAPYTELIDRLREVAALNSAASLLNWDQETMMPERAGASRAEE
                     LALLARLAHERATDDRVGEWIERCREDDVLAADPVRSANLREIARDYERARKLP"
BASE COUNT          574 a         1148 c         1159 g          517 t
ORIGIN      
        1 cgctccggac ggatcggcgg ccggtcgagc tgcgtcgtcg gggaccatgc gcgggccgcg
       61 tcagccgacg aaggagccga tctccttcca gtgggcgagc gtggacggcg cgatgccgaa
      121 gtcgggctgc tcgcgcgcaa cgtcgtagcc ctgcgttcgg agcatcatcg cgatcagcgc
      181 gtagtgatgg atcgtatgac tgacgaggaa ctgcagttcg cgagccacgc tcgagtcgct
      241 ccactcggcc gcgccggccg ccccacactc ggcgcgcacg cgcagcgggc gcgtgccggt
      301 gtcggctgtc agacgcagca gcgtctcgcg caccttgtgg agccgctccg cggcgtgact
      361 gcggtcggtc tcggcgcgcg tatcgcgctt gcgccggttg tagtcgatcg cccgctcagt
      421 gatccccgcc aggaagcaat cgtagaagtc gaggcagtgc cgcagctgcg ctccgactcc
      481 gcctcgcgag cgctcgcccg taccggcggc gtacaactcg tcggtcagat tctcaatcaa
      541 ctccgatccc tggtcgagat agtggatgtt gattcccgcc gggtgtgggt cggggctcat
      601 cggttctcct tcctggctcg atctcgctcc gcggaccgta cccatatcac agttcggaac
      661 tgtcgttcca cctcgcgctg caaccgctgg gtcagcgcct tgcgctcgcg ttcgacgatc
      721 ggctgatcgc cgaactcgat ccgggcctcg aaaccgggga gcgacaacag accgaaaacg
      781 tgggagacga acggcatttt cccccaccag cagaccgcgt cgcgggccgc cgggctgccg
      841 gccggcgtgc gataggcgag gcaggcgtaa tggaccgggc ggccggtgcg ggctgccgac
      901 tcgagcagcg acggacggaa acggagcacg tcgtcgcccg gcgaggaagt gccctccgga
      961 aacagcacga tcccgcggcc cgaggcgagc acccgctcca tctcggtcat cacgcgcgga
     1021 atctcacgtt tctgctcgcg ccggatgaac agcgtgtcga ccgacgagca caaccggccg
     1081 acgaccggcc agtcgcggac ctcggccttg gaaacgaaga cgcaacgaag ctccgaagcc
     1141 aacagaacaa cgtcgacgta gcccaggtgg ttcgagacca atacgaacgg cggacgtgga
     1201 tggtttcctc cggtcgtcac ccgcatgccg agcaggcggg ccatcgtctt gccccaggcg
     1261 tggaacacgc cggtgcgcca ggccgggtcc ccgcggccgg tgcggccgaa cctcgcgagc
     1321 cacaccagtc cgaccgagct gacgacgatg acgccgaaga ataccgcgtt ggcgacgacg
     1381 agcgccagca gcttggctac cccgcggagc cggctcacga gcgacccgtc aagcgaaaaa
     1441 ggtcttgcgg gcgtccgcgc cgaggcgctc gacgtccagc acgaccagat agtcgatcgt
     1501 cttgaagcac cgatcgatcg cgggcgggcc gcagacctgt gctccatagc gcaggtagag
     1561 cgaaaacagc ttgggcagct cgatcttccc ttccggtagc gtggaggccg gcggctcgcc
     1621 ggcatcgtag caacgccagt cgggccgcgg ctcgatacgg aaatcggagc ggacgtgccc
     1681 cgccgcccgc aggtaatcca tcacccgccg gccttcgtcc gggtcctggc tcgtcagcga
     1741 gcagcaaccg aacaggtagc gcttgcggtt gtgcgtcatg taggcggcga gcccgcacca
     1801 gagcaagaac aacacgtaac gattgcggaa cggcttcgcc acgcaggcac ggccgacttc
     1861 gaccgaattg tcgagcagat tgcgcggcat ccgtgacagg tcgaactcat cggccgagta
     1921 atagccgatg tgattcgccg ccatctcgtc ggtctgcatg cgataggtac cgatcacctc
     1981 gtcgttggca tcgagcacca tcagatggtg acagtaggca tcgaagcggt cctggtccct
     2041 gccggtcttg taggattcct cgagtccttc tcccaactcg aggttgaaga cctcgaaccg
     2101 cagctgctga accgacttga gctcggcggg tgaggtcgcg aatcgaaccg agtagcctgc
     2161 cttggccagc ttcaactcgg ggatggactg cggttcgacg ggatagtcgg ctaccgacgc
     2221 tcccttgctc gacgacacct gagcacctcc gcctcccggg tccaggtcgc gcatcgcgca
     2281 ggttagaata cctgcaaatc cgcgaaggag aaagaccgtt gggtcggagg aaacggcgag
     2341 tgaccgcgcg cgggacgatg aagaccgtgg tcgttgtcgc atggaagtga tcccgctccc
     2401 acccgccgac gattatccgt cggtcgagga cggtccggaa gtcgagcacc gcgtgcgtgg
     2461 atcccgcttc ctcggacggg cgttccacgt gcgccgggcg agcgaagccg ccgtgcgggt
     2521 ccgcgaggtg cgccgcgccc accacgcggc ccgtcatcac gcctgggcct cgcgcatcgg
     2581 cccgccgaac gacgtcgtcg agcgagcgga cgacgatggc gagccgtcgg ggaccgccgg
     2641 ccggccgatc ctccagtgca tcggcggccg atcgttgtcc gacgtgctgc tggtcgtgac
     2701 acgctatttc ggcggaacca agctcgggac cggcgggctg gcgcgcgcct acgccgacgc
     2761 ggcccgtctg gcgctcgacg gttgcgtcgg gcgcgtcgtc gagatcgact gcacgatcga
     2821 aatcgtgttg gcctacgccg acctcgggtt cttggaagcc gcgctggccc gatcgatcga
     2881 cgggattcgc cgcgtcagcc gcgagtacga cccgtccccg cgaatcaggc tcgccgtgcg
     2941 gccgacccgg gccgcgggca tcgccgcatc gatcatcgac gcgacgggcg ggcgagcgag
     3001 cgtgtcgctc gtccggtcgg cgcttgcacg cggtccgggg gctgtctaga ctgcgcgcaa
     3061 ccggccggat gacccggtca cgcgacctgc gggagagcac gagcgatgtc ggccccctac
     3121 accgagttga tcgaccgtct gcgggaagtg gccgcgctga actctgcggc cagtctgctc
     3181 aattgggacc aggaaacgat gatgcccgag cgcgcgggcg cgtcgcgcgc cgaggagttg
     3241 gcgctgctcg cccggctggc gcacgagcgc gcgaccgacg atcgggtggg cgagtggatc
     3301 gagcgctgcc gtgaagacga cgtgctcgcc gcggacccgg tgcgctcggc caacctgcgg
     3361 gagatcgcac gcgactacga gcgcgcgcgc aagctgcc
//
LOCUS       DASPGN010000305        10658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_639971, whole genome shotgun sequence.
ACCESSION   DASPGN010000305 DASPGN010000000
VERSION     DASPGN010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10658
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639971"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VD788_08815"
     CDS             <1..403
                     /locus_tag="VD788_08815"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXV76409.1"
                     /translation="PHLDRTLHRIKELGARAGVALNPSTPVGTLDEVLDLTDYVLIMT
                     VNPGFGGQRLIPSALRKVASLREKTAGRRPALRIGVDGGVTEQNLAQVARSGADVIVA
                     GSAVYGDPDPQAATRRIVRRLAEVAERERSC"
     gene            387..830
                     /locus_tag="VD788_08820"
     CDS             387..830
                     /locus_tag="VD788_08820"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXV76410.1"
                     /translation="MSEAADRPARAHRTCIRVRYAETDRMGIAWHGRYLEWYETGRTE
                     LMRAAGCTYADVEDEAGIRFPVIEVGSRHLRPARYDDVVEIYTRVTEVRGARVRFDYE
                     LVRASDRQTLATGYTEHAAVGSDGRPTRLPPHLRARLVSSRPDET"
     gene            820..1671
                     /gene="bamD"
                     /locus_tag="VD788_08825"
     CDS             820..1671
                     /gene="bamD"
                     /locus_tag="VD788_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HXV76411.1"
                     /translation="MRPERRAGARRATPSRRSSAILLSVLALCAGASAGCGKKNKTQT
                     TGMIDARQVYAESMRQLQRDNLTEAQSLLERIAYADEDREELEPLVRLAIADATFYES
                     RGINLIDARSLYLDFVALYGAHPLAPYAQMQAGVCSLLQVANPSRDQTLTHQAVTDLR
                     VTEQRWPDSAFARAASTMIRQAEANLAEHEFIVGRFYLNRKARLAAVERFQRILDRYP
                     HYREKDKVYFHLGEAQLGLGNEAEARIYLEKLVTDYPDGEFARSARRELKQLDRQAPQ
                     FDVAGGR"
     gene            complement(1712..2596)
                     /locus_tag="VD788_08830"
     CDS             complement(1712..2596)
                     /locus_tag="VD788_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXV76412.1"
                     /translation="MTRVRIGIAGWDYPDWIGPVYPAAARGGVDRLAFVARYVDLVEV
                     NSTFYRPVAARVAVSWVRRTAARGESFAFSAKAHRSWSHEVGPPGAETIAATLDGLRP
                     IREAGKLVALLVQFPQSFRPDDRALARLERLRAATGDWPLAIEVRHADWQRDETAEWF
                     GRSGLAWCVVDQPRMDGSTADGRARVTAPVGYLRLHGRNEQNWFRADAGRDARYDYRY
                     SRTEIAEVAGRVRQMESRCDELVVVQNNHFRGQALANALELKELIERRKPLAPRTLVD
                     AFPDLAARVRVEASGRLF"
     gene            2701..4878
                     /locus_tag="VD788_08835"
     CDS             2701..4878
                     /locus_tag="VD788_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HXV76413.1"
                     /translation="MPAARSTSQSRSARSKRRQPRERRSERHPLDAIFRPRSVAIVGA
                     SRRTHSIGREILHNLIDFGFSGPCYPINPNATAVLSIPTYRTLADVPGPIDLAVIVVP
                     RDHVLQVVDECAKKGVNGIVVITAGFKEVGEEGRQLESRLKRKLERHGIRMVGPNCMG
                     VINTEKSIRLNATFAAAVPPRGNVGFISQSGALGEAILANASGAGIGVSMFVSMGNKT
                     DISGNDLLEYWEHNDDVQVILMSLESFGNPRRFTQLARRVTRKKPIVTVKAGRTAAGA
                     RAATSHTGSIVGLDVATDSLLEQCGVLRVNSLEQMFVQAAALANQPVPRGGRIAIVTN
                     AGGPGILCTDSLSGRGVELAEFRPATRKALRKALPPEASTANPVDMIASADARQYRAV
                     LELVKRDPGVDGIIAIFVSPIMIDAFEVARAIAEASDGSKPVLSVFMGKNRSREGLQE
                     LARHRVPVYRFPEEAASAMGAMVNYQRLRDAPLGTEVKFEVDRARALRAIRSARHASR
                     SALRAAEVDEVLAAYGLPIAPSVRVRSAAEAIAAAQAMGYPVVLKIDSERYSHKTDVG
                     GVKVDLRNGDEVAAACGELKAALGRRDPTLEFRVQRMVRGGREVILGMTRDPQFGALI
                     LFGLGGVFVEVMRDVSIRIHPLTDVDARSMVERVQGYPLLAGTRGDKPVAIDLIEECL
                     LRLSQMIGDLEEHLAELDVNPLIVTDRREGSFVVDARISLSEG"
     gene            complement(4958..5377)
                     /locus_tag="VD788_08840"
     CDS             complement(4958..5377)
                     /locus_tag="VD788_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76414.1"
                     /translation="MNQTASANRTRSSHRIGRWAALAVTALLLATAADAHPRHHKHHG
                     KHGHGHHSRHVVARVAPRPFIVPEVISLRYVDSYRPYFDGRVYHREHRHYHDTYAFPV
                     YVDGHWQTASHSYCSGRRLPDSYVSFSGPRVSFGIRF"
     gene            5598..6140
                     /gene="hslV"
                     /locus_tag="VD788_08845"
     CDS             5598..6140
                     /gene="hslV"
                     /locus_tag="VD788_08845"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HXV76415.1"
                     /translation="MNRNAEIVATTVLAVRRGKSVVLAADGQVTLDRAVVKSGARKVR
                     RLGDGSVLAGFAGGAADAFALLTRFEEKLAERHGQLERAAVELVRDWRTDRALRRLEA
                     MLIVADRAKTLVLSGTGDLIEPDDGVVAIGSGGVAAMSAARALLRHTDLGPAEVAREA
                     MKIAASIDIYTNDSLTVEEI"
     gene            6140..7546
                     /gene="hslU"
                     /locus_tag="VD788_08850"
     CDS             6140..7546
                     /gene="hslU"
                     /locus_tag="VD788_08850"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003740291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HXV76416.1"
                     /translation="MVFYMPGQVELPPRSAEELTPRQTVAELDRFIVGQAKAKRAVAI
                     ALRNRVRRRRLSPELADEVMPKNILMIGPTGVGKTEIARRLARLSGSPFVKVEASKFT
                     EVGYVGRDVESMVRDLVEVGIEMVRQEKRAEVRERARRAVEQRLLDLLLPPRKRLSRI
                     GDPAAEEREREEQASWERSRETLKRKLAAGELEDRTVEVEVNASPAGGMRIFSAAGME
                     ELDMNLKEMMPGLFQGKPRLRKMNVSEARQVLQREEEDKLVDPDQLARDAIRRVEQSG
                     IIFVDEIDKIAGREGGHGPDVSREGVQRDLLPIVEGTLVHTKHGMVRTDHVLFVAAGA
                     FHVSKPADLIPELQGRLPIRVELESLGRDELVRILTEPENALVRQYQALLKTEGVELV
                     FTADGVVAIAEAAALVNTRTENIGARRLATVMERLLDEVSFKGCELAGQRIEIDATYV
                     EQRLSGIVQDADLSRFIL"
     gene            7573..8685
                     /locus_tag="VD788_08855"
     CDS             7573..8685
                     /locus_tag="VD788_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXV76417.1"
                     /translation="MARDRRRLSCSIPAWSTTLALLLGSGGCGEKGPPLPPEPRGPFP
                     PEQVRVRQVGAEVRVGFVVPVARGGKQAQQPVRAELVRVTYPPGIQPTGGADPFRRRG
                     EIVAVIDGDPLPAGEARTIVDDTLDGMADGARDYTTRYGVRVRDRRGRPSPLVVAADL
                     TIASVARAAGELRGEATADGIRLAWAAPPGEGPFRYNVYRLAGEQSSALDEPLNRRPL
                     EETEYLDTTVATGERYTYLVRVALSEGRPYVEGEDGAAITLLAQDRFAPSSPVGLVAV
                     QEGVGVRLFWDPNPERDIRGYRVYRRVDEGEVEGLWEPLGTDPIERPLYLDTDVRVGQ
                     RLVYRVTAVDRATPPNESAPSASQTIDLRTEPGVGS"
     gene            8682..9512
                     /gene="dapF"
                     /locus_tag="VD788_08860"
     CDS             8682..9512
                     /gene="dapF"
                     /locus_tag="VD788_08860"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXV76418.1"
                     /translation="MSGFEIVKMSGSGNDFVVLDGVGRERLGADVGAWVRRICRRGLS
                     VGADGVLFVRPLGESAVEVEFRNPDGSLAFCANGTRCAARFAFRRGMAPRRMTLETAA
                     GTIPAVVDEHAVRLELPVPVDSGTREVAVGDERVRGRLVDAGVPHFVIDDVDLPEGTL
                     GRWGPVVRRAPVFGAQGTNVDLARRTGRRELSVRTWERGVEGETLSCGSGAVASAFVA
                     WLGGIGRSVRVVPPSGVPLEIEFDDAVGELRAVRLSGDARTVFVGQVDDESVSGFDAG
                     "
     gene            complement(9537..10478)
                     /locus_tag="VD788_08865"
     CDS             complement(9537..10478)
                     /locus_tag="VD788_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV76419.1"
                     /translation="MEFARRSTRRAACSIAAVCACSLTLVATADPPAFESRELAESIY
                     LMRPAGGRSDLANSLLVVRHDGLLVVDAQATPEAARELLAAIAAVSRAPIRYLVLSHA
                     HAEAAGGASAFPESTLVIASTRARDALGDPEWDFAAELDGGMTGADARKPVLVLQART
                     ELDDPQNRIEIFPLGHAHTNTDLFVVVPRANLIYAGAVLFDRQPYARDADVGGWLAAL
                     NYLTKLRPAIVVPLHGEPTDVSGVRKARESLAWLRGRVELGFIDGIAPERIPDRVLAD
                     EEFDDYFGANVSSSVARGLIEQAVAEAIEQRRKRGIM"
BASE COUNT         1685 a         3516 c         3848 g         1609 t
ORIGIN      
        1 accgcatctc gaccgcacgc ttcatcggat caaggagctc ggggcgcggg ccggggtggc
       61 gctcaacccg tcgacgccgg tcgggacgct cgacgaggtg ctcgatctca ccgattacgt
      121 gctgatcatg acggtcaacc cgggattcgg cggccagcgc ttgattccgt cggccctgcg
      181 gaaggtcgcc agcctgcgcg agaaaaccgc gggccggcgc ccggcactgc gcatcggcgt
      241 cgacggggga gtgaccgagc agaacctcgc gcaggtcgcc cgatccggcg ccgacgtgat
      301 cgtcgccggc tcggcagtct acggcgaccc cgacccccag gccgcgaccc gacgcatcgt
      361 tcgacgcctg gcggaagtgg cggaacgtga gcgaagctgc tgaccggccc gcccgcgccc
      421 accggacgtg catccgcgtg cgctacgccg agaccgatcg gatgggcatc gcgtggcacg
      481 gccgctacct cgagtggtac gagacgggac ggaccgagct gatgcgggcc gccggctgca
      541 cctacgccga cgtcgaagac gaggcgggta ttcgctttcc cgtgatcgag gtcggttcgc
      601 gtcatctcag accggcccgc tatgatgacg tggtggagat ttacacccga gtgacggagg
      661 tccgcggggc tcgcgtgcgg ttcgactacg agttggtgcg agcgagcgac cggcagaccc
      721 tggccaccgg ctacaccgag cacgccgccg tcggctcgga cggacgcccg acccgactgc
      781 cgccccacct ccgcgcacgt ctcgtgtcgt cgagaccgga tgagacctga acgacgagcc
      841 ggtgcccgcc gcgcgacgcc gtcccgccgg tccagtgcca tcctgctgag cgtgcttgcg
      901 ctctgcgcgg gggcgtcggc cgggtgcggt aagaagaaca agacgcagac gacggggatg
      961 atcgatgcac ggcaggtcta cgcggagagc atgaggcagc tccagcggga caatctgacc
     1021 gaagcgcaat cgctgctcga acggatcgcc tacgccgacg aggatcggga ggagttggag
     1081 ccgctcgtgc gtctggcgat cgccgacgcc acgttctacg agagtcgggg gatcaacctg
     1141 atcgatgccc ggtcgctcta tctcgacttc gtggcgttgt acggcgccca tccgctggcg
     1201 ccctacgcgc agatgcaagc cggtgtttgt tcgctgctgc aggtggccaa cccgtcgcgc
     1261 gaccagacgc tgacgcatca ggcggtgacc gacctgcgcg tgaccgagca gcgctggccg
     1321 gactcggcct tcgccagagc ggcctcgacg atgatccgtc aggccgaggc caacctcgcg
     1381 gagcacgagt tcatcgtcgg caggttctac ctcaaccgca aggcccggct ggcggccgtc
     1441 gagcgcttcc agcggattct cgatcgctat ccgcactacc gcgagaagga caaggtctat
     1501 ttccacctcg gcgaggctca gctcggtctg ggcaacgagg ccgaggcccg gatctacctc
     1561 gagaaactgg tgaccgacta cccggacggc gagttcgccc gaagcgcgcg gcgcgagttg
     1621 aagcaactcg accggcaggc tccacagttc gacgtcgcgg gtgggcgctg acgccgtcgg
     1681 gcgacggagg cggcgcaggc tcgggcgacc gttagaacaa ccgccccgag gcttcgacgc
     1741 gcacccgagc ggcgagatcg ggaaacgcgt cgaccagcgt tcgtggcgcc agcggcttgc
     1801 gccgctcgat cagctccttc agttcgagcg cgttggccag cgcctggccg cggaagtggt
     1861 tgttctgcac caccaccagc tcgtcgcagc gcgactccat ctgccgcacg cgcccggcga
     1921 cctccgcgat ctcggtgcgg gagtagcggt agtcgtagcg cgcgtcgcgc ccggcgtcgg
     1981 cacgaaacca gttctgctcg tttcgcccgt ggaggcgcag atagccgacg ggcgcggtca
     2041 cccgggcgcg gccgtcggcg gtcgagccgt ccatccgcgg ctggtcgacg acgcaccacg
     2101 cgagaccgct gcggccgaac cactccgccg tctcgtcacg ctgccagtcg gcgtgtcgga
     2161 cctcgatggc gagcggccaa tcgccggttg ctgcgcgcag ccgctcgagc cgtgcgagcg
     2221 cgcggtcgtc cgggcggaac gactgcggga actggaccag caacgcgacg agcttcccgg
     2281 cctcgcggat cggccgcagt ccgtcgagcg tcgcggcgat cgtctccgct cccggcgggc
     2341 cgacctcgtg agaccaactg cgatgggcct tggcgctgaa ggcgaacgac tcgccgcgag
     2401 cggccgtccg tcgaacccac gagaccgcga cgcgggcggc caccgggcgg tagaaggtgc
     2461 tgttgacctc gaccaggtcg acgtagcgtg cgacgaacgc gagccggtcc acgccgccgc
     2521 gcgcagcggc cgggtagacc ggcccgatcc aatcgggata gtcccacccg gcgatgccga
     2581 tacggacccg ggtcacggct cgcattgtgc aaaccggtcc gcgcacggtc aagccgcggc
     2641 ggatgcgcgg tgctatcctg cgtccctctc ggcccgatcg cgggcgcgcg ggggagcacg
     2701 atgccggcgg cacgcagcac gagccagtcg cgatccgcgc ggtcgaagcg ccggcagccg
     2761 cgcgagcgac ggagcgagcg tcacccgctc gacgccatct tccggccacg ctcggtcgcg
     2821 attgtcggcg cgtcgcgtcg cacgcactcg atcggccgcg agatcctgca caatctgatc
     2881 gatttcggtt tttccggtcc gtgctacccg atcaacccca acgccaccgc cgtgctgtcg
     2941 atcccgacct accggacgct ggccgacgtg ccgggcccga tcgacctggc ggtgatcgtc
     3001 gttccccgcg accacgtcct gcaggtcgtc gacgaatgcg cgaagaaggg cgtcaacggc
     3061 atcgtcgtca tcactgccgg cttcaaggag gtcggcgaag agggccggca gctcgagtcg
     3121 cggctcaagc gcaagctcga gcgtcacggc atccggatgg tcgggccgaa ctgcatgggc
     3181 gtgatcaaca cggaaaagtc gatccgcttg aatgccacct tcgccgccgc ggtgcctccg
     3241 cgcggcaacg tcggcttcat ctcgcaatcg ggcgcgctgg gcgaggcgat cctggccaat
     3301 gcgtcggggg ccggcatcgg cgtgtcgatg ttcgtgtcga tgggcaacaa gaccgacatt
     3361 tccggcaacg acctgctcga gtactgggag cacaacgacg acgtgcaggt gatcctgatg
     3421 tccctcgagt cgttcggcaa cccgcgccgc ttcacccaac tggcgcgaag agtgacgcgc
     3481 aagaagccga tcgtcaccgt caaggccggc cgcacggcgg ctggcgcgcg ggcggccacc
     3541 tcgcacaccg gctcgatcgt cggcctcgac gtggcgaccg attcgttgct cgagcagtgc
     3601 ggcgtgttgc gcgtcaattc gctcgagcag atgttcgtcc aggccgcggc actggccaac
     3661 cagcccgttc cacgtggtgg tcgtatcgcg atcgtcacca acgcgggcgg accgggtatc
     3721 ctctgcaccg attcgctgag cgggcgcggg gtcgaactgg cggagtttcg tccggcgacg
     3781 cgcaaggcgt tgcgcaaggc gctgcccccc gaggcgtcga cggcaaatcc ggtcgacatg
     3841 atcgcttcgg ccgacgcgcg gcagtaccgc gccgtgctcg aactggtcaa gcgcgacccg
     3901 ggcgtcgacg ggatcatcgc catcttcgtc tcgccgatca tgatcgacgc gttcgaggtc
     3961 gcgcgcgcga tcgccgaggc atcggacggg tcgaagccgg tgctgtcggt gttcatgggc
     4021 aagaaccgct cgcgcgaagg tctgcaggag ctcgcgcgtc accgtgttcc ggtctatcgc
     4081 tttccggaag aggcggcatc ggcgatgggg gcgatggtca actaccagcg tctgcgggac
     4141 gcaccgctcg gcacggaagt caagttcgaa gtcgaccgcg ctcgagcgct gcgcgcgatc
     4201 cgctcggcgc gtcatgcctc ccgctcggcg ttgcgtgcgg cggaagtcga cgaggtgttg
     4261 gcggcctacg gcttgccgat cgcgccatcg gtgcgcgtcc gatcggcggc cgaggcgatc
     4321 gccgcggcgc aggcgatggg ctatccggtc gtgctcaaga tcgactccga acggtactcg
     4381 cacaagaccg acgtgggcgg ggtgaaggtc gacctgcgta atggcgacga ggtcgcggcg
     4441 gcctgcggcg agctgaaggc cgcgctcgga cggcgcgatc cgacgctcga gtttcgcgtg
     4501 caacggatgg tccgcggcgg acgggaggtc atcttgggga tgacccgcga cccgcagttc
     4561 ggcgcgttga tcctgttcgg tctcggcggc gtcttcgtcg aggtcatgcg tgacgtgtcg
     4621 atccgcatcc acccgctgac cgacgtcgac gcgcgctcga tggtcgagcg cgtccagggg
     4681 tacccgctgc tggccgggac ccgcggcgac aagccggtcg cgatcgacct gatcgaggaa
     4741 tgcctgctgc ggttgtcaca gatgatcggt gatctcgagg agcacctggc ggagctcgac
     4801 gtcaacccgt tgatcgttac cgaccggcgc gaggggtcgt tcgtggtcga cgcgcgcatc
     4861 tcgctgagcg agggctgacc cccgaggccg gcgagcggtc gccccccacg cgccacggtg
     4921 gccgaacgct cgccgggcgc gcggggcgcg agaccgacta gaagcggatt ccgaacgaca
     4981 cgcgggggcc gctgaacgag acgtagctgt ccggcaggcg gcgacccgag cagtaggaat
     5041 gcgacgccgt ctgccagtgc ccgtcgacgt agaccgggaa ggcgtaggtg tcgtggtagt
     5101 ggcggtgctc gcggtggtag acccgcccgt cgaagtacgg gcggtagctg tcgacgtagc
     5161 gcagcgagat cacctccggc acgatgaacg gccgcggcgc gacacgcgcg acaacgtgcc
     5221 tcgagtgatg accgtgcccg tgcttgccgt ggtgtttgtg atgtcgcgga tgggcgtcgg
     5281 cggcggtcgc caacaacagc gccgtgacgg cgagcgcggc ccaacggccg atacggtgcg
     5341 aagatcgagt tcggttggcg gatgctgtct ggttcatggc gttcacctcc gtacggaagg
     5401 ggaggtagcc agattcgggc caaccctcgg ccgctcgaca caacttgctg agggcgtgtg
     5461 agttgaatcg ggtcccgcgc ggggagatcg ccgtccggag cgagaggaac gtcctctggc
     5521 gaggacggga cgcgcacact ccgggggcgg gtagcgtcgt ctccggggtc cgggatacac
     5581 tgcgattttg gcatgtcatg aatcgaaacg ccgagatcgt cgccaccacc gtcctcgccg
     5641 tgcgccgcgg gaaaagcgtg gtgctggccg ctgacggtca ggtgacgctc gaccgcgcgg
     5701 tggtcaagag cggtgcgcgg aaagtccgac gcctcggtga cggctcggtc ctcgccggct
     5761 tcgccggtgg cgcggccgat gcattcgcgc tgctgacgcg cttcgaagag aagctcgccg
     5821 agcgtcacgg gcaactcgag cgagccgccg tcgaactggt gcgcgactgg cgaacggacc
     5881 gcgccctgcg gcgcctcgag gcgatgctga tcgtggccga ccgggccaag acgctcgtgc
     5941 tctccggcac cggcgacctg atcgagccgg acgacggcgt ggtggcgatc ggcagcggag
     6001 gcgtggcggc gatgtccgct gcccgcgccc tgctgcgcca tacggacctg ggaccggccg
     6061 aggtcgcgcg agaggcgatg aagatcgccg cgtcgatcga catctacacc aacgacagcc
     6121 tgaccgtcga ggagatttga tggtcttcta catgcccggt caggtcgaac tcccgccgcg
     6181 atcggccgaa gagttgactc cgcgacagac ggtcgcggag ctcgaccgtt tcatcgtcgg
     6241 acaggcgaag gccaagcgcg cggtggcgat cgccctgcgc aatcgagtac gtcgtcgccg
     6301 cctgtccccc gagctggccg acgaggtcat gccgaagaac atcctgatga tcgggccgac
     6361 cggcgtggga aagaccgaga tcgccaggcg cctggcacgt ctgtccgggt cgccgttcgt
     6421 caaggtcgaa gcgtcgaagt tcaccgaggt gggctacgtc gggcgggacg tcgagtcgat
     6481 ggtccgcgac ctggtcgagg tcgggatcga gatggtgcgt caggagaagc gggccgaggt
     6541 gcgcgagcgg gcgcgccgcg cggtcgagca gcggttgctc gatctgctgc tgccgccgcg
     6601 caagcgcctg tcgcggatcg gcgatcccgc ggcggaggaa cgcgagcgcg aagagcaggc
     6661 gtcttgggaa cggagccggg agacgctcaa gcggaagctc gcggcgggtg agctcgagga
     6721 ccgtacggtc gaggtcgagg tcaacgcctc gccggccggg ggtatgcgca tcttcagcgc
     6781 ggccggcatg gaagagctcg acatgaacct gaaggagatg atgccgggtt tgttccaggg
     6841 caagccgcgt ctgcgaaaga tgaacgtgag cgaagcgcgg caggtgctgc agcgcgagga
     6901 ggaggacaag ctcgtcgatc ccgatcagct cgcgcgcgac gcgatccggc gcgtcgagca
     6961 gtcgggcatc atcttcgtcg acgagatcga caagatcgcc ggccgcgagg gaggacacgg
     7021 tcccgacgtc tcacgcgaag gcgtgcagcg cgacctgctg ccgatcgtcg aggggacact
     7081 cgtccacacg aagcacggca tggtgcgcac cgaccacgtc ctgttcgtcg ccgccggggc
     7141 gtttcacgtg tcgaagcccg ccgatctgat tccggagctg caaggacgct tgccgatacg
     7201 cgtcgaattg gagtcgctcg ggcgcgacga gctggtgcgc atcctgaccg agcccgagaa
     7261 cgcactcgtg cggcagtatc aggcgttgtt gaagaccgag ggcgtcgagt tggtgttcac
     7321 cgcggacggc gtcgtggcga tcgccgaagc ggcggcgctg gtcaacacgc ggaccgagaa
     7381 catcggcgcc cgacggctgg cgacagtgat ggagcgattg ctggacgagg tctcgttcaa
     7441 gggttgcgaa ctggcgggcc aacggatcga gatcgatgcg acctacgtcg agcagcgcct
     7501 gtccggcatc gtgcaggatg cggatctgtc tcgtttcatt ctctgatcga cgaccgcagc
     7561 ccggagatcg gcatggcccg cgaccgacga cgactgagct gctcgatccc ggcttggtcg
     7621 accacgttgg ctctcctgct cggaagcggc ggctgcggcg agaaggggcc gcccctaccg
     7681 cccgaacctc gcggaccgtt cccgccggaa caggtgcgcg tgcgacaggt cggcgcggag
     7741 gtgcgagtcg gcttcgtcgt tccggttgca cgcgggggca aacaggcaca gcaaccggtc
     7801 agggccgagc tcgtgcgcgt gacctaccct cccgggatcc agccgaccgg tggagccgat
     7861 ccgttccgac gtcgcggtga gatcgtcgcc gtaatcgacg gcgacccgct gcctgccggc
     7921 gaggcgcgca cgatcgtcga cgacacgctc gacggaatgg cggacggcgc tcgcgactac
     7981 acgacacgct acggcgtgcg cgtccgcgac cggcgcggca ggccctctcc gctcgtggtc
     8041 gccgccgacc tcaccatcgc ctccgtcgca cgagccgccg gcgagctgcg cggcgaggca
     8101 acggccgacg gtatacgcct ggcgtgggct gctccgcccg gcgaggggcc gtttcgttac
     8161 aatgtctacc gcctggccgg cgagcaaagt tcggcgctcg atgaaccgct caaccgccgg
     8221 ccgctcgagg agacggagta cctggacacg acggttgcga ccggcgagcg ttacacctac
     8281 ctggtgcgcg tcgcgctatc cgagggtcgc ccgtacgtcg aaggcgaaga cggcgcggcg
     8341 atcacgttgc tcgcgcaaga ccggttcgcc ccgtcgtcgc cggtcggcct ggtcgcggtg
     8401 caggaagggg tcggtgtgag gctgttctgg gacccgaatc ccgagcgcga catccgcggc
     8461 taccgcgtgt accgccgggt cgacgaaggc gaagtcgagg gcctctggga gccgctcggc
     8521 accgatccga tcgagcgtcc gttgtatctc gacaccgacg tccgggtcgg acaacgactc
     8581 gtctaccgcg tgaccgccgt cgatcgtgcg acgccaccga acgagagcgc gccatccgcg
     8641 agccagacga tcgatcttcg tacggagccg ggagtgggct cgtgagtgga ttcgagatcg
     8701 tcaagatgtc ggggagcgga aacgacttcg tcgtgctcga cggcgttggg cgagaacgtc
     8761 tcggcgcaga cgtcggtgcg tgggtccggc gcatttgtcg ccgcgggttg tcggtgggcg
     8821 cggacggcgt gctgttcgtg cggccgctcg gcgagtcggc ggtcgaggtc gagttccgca
     8881 atcccgacgg gagcctggcg ttctgcgcca acggcacgcg ttgcgcggcg cgtttcgcgt
     8941 ttcggcgcgg gatggcgccg aggcgaatga cgctcgaaac ggcggccggc acgatcccgg
     9001 ccgtcgtgga cgagcacgcg gttcggctcg agctgccggt gccggtcgat tcgggaacgc
     9061 gcgaggtggc ggtcggcgac gagcgggtga ggggtcgcct ggtcgacgcc ggcgtgcccc
     9121 acttcgtgat cgacgacgtc gatctgcccg agggaacgtt gggtcgctgg ggtccggtcg
     9181 ttcgccgcgc tccggtgttc ggtgcgcagg gcaccaatgt cgacctggct cgccggacgg
     9241 gccgccgcga gttgagcgtc cgaacgtggg agcgtggtgt cgagggcgag acgttgtcct
     9301 gcggcagcgg cgccgtcgcg tcggcgttcg tggcatggct gggcggaatc ggccgctcgg
     9361 ttcgtgtcgt accgccgagc ggcgtcccgc tcgagatcga gttcgacgac gcggtgggcg
     9421 agctgcgcgc ggtgcggctg agcggcgacg cgcgaacggt gttcgtcgga caggtggacg
     9481 acgaaagcgt ctctggcttc gacgccggtt gaaccgcgtc cggattcgcg agccggctac
     9541 atgatgccgc gcttacggcg ttgctcgatc gcttcggcca cggcctgctc gatcagtcca
     9601 cgggcgaccg acgaggaaac attcgcgccg aagtagtcgt cgaactcctc gtcagccaag
     9661 acccggtcgg ggatccgctc cggcgcgatc ccgtcgatga accccagctc gacgcgtccg
     9721 cgaagccagg ccaagctctc cctcgctttc cgcacaccgc tgacgtcggt cggctcgccg
     9781 tgcagcggaa ccacgatcgc cggtcgcagc ttggtcaggt agttcaacgc ggccagccac
     9841 ccgccgacgt cggcgtcgcg cgcatacggc tggcggtcga acagcaccgc gccggcgtag
     9901 atcaggttgg cccgcggaac gacgacgaac agatcggtat tggtgtgggc gtggccgagc
     9961 gggaagatct cgatcctgtt ctgcggatcg tcgagctcgg tgcgggcttg caacaccagc
    10021 acgggtttcc gcgcgtcggc gcccgtcatc ccgccgtcga gctcggccgc gaagtcccac
    10081 tccgggtcgc cgagggcgtc acgcgctcga gtcgaggcaa tgacgagcgt cgattccggg
    10141 aaggccgagg caccgccggc cgcctccgcg tgggcgtgtg acaggacgag gtaacggatc
    10201 ggcgcccggc tgaccgcggc gatcgccgcg agcagctcgc gggccgcctc cggtgtcgcc
    10261 tgagcatcga cgaccagcag gccgtcgtgc cgaacgacga gcagcgaatt ggccagatcg
    10321 gagcgaccgc ccgccgggcg catcaggtag atcgactcgg ctagttcccg tgactcgaaa
    10381 gccggcgggt cggccgtcgc gaccagcgtc aagctgcacg cgcacacggc cgcgatcgag
    10441 cacgccgcgc gcctggtaga ccgacgggcg aattccatcc tcgtctcccc gcgacaggaa
    10501 gaaaaactac catcggtcga ggaggagggc caccgcaccg ggcgtttcag cgggtgagcg
    10561 accggggtat cagcgaagca tcgcccccgg cgaggacgtc gacgaatgcc tggtgcagga
    10621 tgcgcaggcc gtcgttgccg agatctccgt gccgcctg
//
LOCUS       DASPGN010000306        11634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_171788, whole genome shotgun sequence.
ACCESSION   DASPGN010000306 DASPGN010000000
VERSION     DASPGN010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11634
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171788"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..960
                     /locus_tag="VD788_08870"
     CDS             145..960
                     /locus_tag="VD788_08870"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXV76420.1"
                     /translation="MKRLVTAAVLVPLLYGAVKLAPPWVFFGVAVLAIGGSCFECYRI
                     MRTCGAAPMVWPGVAAAAATVWLYGDLPPHLPPSLPLVALVVVVTPVAIWTRRAPDAV
                     LRTIVDTIFPVAFVGLTMGYVVALRALPDPDGEDLVTLLFICLILADTAAYYVGTRLG
                     RHKMSPQISPNKSWEGFAGAMAGAIVGTALAHSWFYTRLPLSHVVPLGLLLGITGALG
                     DLAESVVKRSCHVKDSSAIFPGHGGIFDRTDNLLIAAPVLYYYYLWLLPGAVG"
     gene            957..2126
                     /locus_tag="VD788_08875"
     CDS             957..2126
                     /locus_tag="VD788_08875"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:NF009114.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXV76421.1"
                     /translation="MTSKRLAVLGSTGSIGVSTLDVVAAHPGRFDIVALAAGDNLERI
                     ERQIRRFEPECVSVRDAEVGAELRRRIGRACRIVHGLDGAVTVATWPGVDMLVSAIVG
                     AVGLIPTHEALGIGRDVALANKESLVVAGAHMTRQAEENGAAILPVDSEHNALHQCLR
                     GERLAEIRHLWLTASGGPFRGLTAQQLAQVDRAQALAHPTWKMGPKITIDSASLMNKG
                     LEVIEARWLFGLPPDRIRVVVHPKSVVHSLVEFVDGSFKAQLGVTDMRHPIQYALSYP
                     ERWESTLPPFDPVAAGPLEFEHPDFERFPCLALAFRALDIGGAAPAVVNAANEVAVQA
                     FLDERIAFVDIPRAVSAALERHAGARSDSLEELIAVDRAARRSTAEWIGQGVGSR"
     gene            2123..3436
                     /gene="rseP"
                     /locus_tag="VD788_08880"
     CDS             2123..3436
                     /gene="rseP"
                     /locus_tag="VD788_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HXV76422.1"
                     /translation="MIKLLEFVLAFVLVLAPLVFIHELGHFLAAKAFRVGVSVFSLGF
                     GPRLAGFRRRETDYRISLIPLGGYVRMIGDESDEHRSGASNEFLSKPRWQRFIVFIAG
                     AAFNIVLAFVVMWFYFATFGKIDAPYPEVYAVAEGSEAEASGVRRGDKLVEISGNNVQ
                     GTRDFLYHYNVEIALAPETLKTIVVERDGERVPLRLNTGLDEKLGIGDPGWGVGRGGN
                     PVIGEVFADGAAAEAGLQPGDRIVAAGSKTSITGLELQVLIEASVGREVRLLIERDGR
                     RLPAVVRPRATEDGGKIGISFVTEPLEPVPLDVGQAAVESFRWNLDLSKTLFVVLKRM
                     VTTELSIKTMSGPVGIAQVARQALLTGVDTFLYLLAFFSLQLGILNLMPIPILDGGHI
                     LILIVEGVMRRNLSEAVKERVMQAGLVFLIAFMGVVIFLDVVKIL"
     gene            3661..3993
                     /locus_tag="VD788_08885"
     CDS             3661..3993
                     /locus_tag="VD788_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXV76423.1"
                     /translation="MKIAKNPLVRRAAAVLAVVSLAAAVAAAPSGSADSTETPRPPAA
                     KIDLNQATQEQLESIPGIGPALAKRILDFRTKEGKFLRVDDLLKVRGIGEKSLEKLRP
                     YVTVEPRA"
     gene            4055..4642
                     /locus_tag="VD788_08890"
     CDS             4055..4642
                     /locus_tag="VD788_08890"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXV76424.1"
                     /translation="MKRKGYTLVELLVALAIVAIIASTATVALADRLGGHAATAGARE
                     LSATLQSLRFRAVATNRHHGLYFERDATGWFWFVVRDENGNGLRTREILDGVDRRLSG
                     PHRLRDRSRGIDLGFPAVTIPALPPRTGSIDDLDDPVKFGRADLVSFAPLGTASGGTL
                     YLTDGRYALRAVVLFGPTARVRVWRFDTREGRWRL"
     gene            4630..5013
                     /locus_tag="VD788_08895"
     CDS             4630..5013
                     /locus_tag="VD788_08895"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HXV76425.1"
                     /translation="METLKSDRRIDVFRSTDSFAVEAYRAATQIRSNVGAGLAEEIRR
                     TAVAAGGAMIAASSYPAGAGAERVHLERARAALLESRYYLHLARRFGWLDAKSYRALT
                     LRQDAAVRGLDGLIGHGSQHARRER"
     gene            5109..5906
                     /gene="ccsA"
                     /locus_tag="VD788_08900"
     CDS             5109..5906
                     /gene="ccsA"
                     /locus_tag="VD788_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXV76426.1"
                     /translation="MDLTSASALLPVTVIGYIVAVVTVTIRTIYRAAAARRLSSAMFV
                     LTWVLHSATVVQLGLANGRLPLATGAEYLLVLGWVVLTLHLYVWFKLSMEVAGVVLPP
                     LAGAMTFGAMQLIGVATPAAVTRDAWFLFHTTVSTLGIAILSVAFAMSVLYLLQDRAL
                     KSKKTLAMLERLPSLQKADQIGFRSLTIGFVLLTLGIATGVVVNAEVYQRLVVLGPKQ
                     VFPLLAWLIFAGVLIARPTIGFRGRKSAYLTITGFAFGLMTVIGMTL"
     gene            5910..7256
                     /gene="hemA"
                     /locus_tag="VD788_08905"
     CDS             5910..7256
                     /gene="hemA"
                     /locus_tag="VD788_08905"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HXV76427.1"
                     /translation="MLVLVGSNHRSAPLAVRERMSFPLEKISEAHTRLMQLDGVREGM
                     ILSTCNRVEILAHTDADGAQSLQRFLADQHRLSLDEVERYTYRLADRDAIRHLFSVAS
                     GLDSMIIGEPQILGQVKQAYQMAKDLRTLGPALERLLQHGLATAKRVRSETGISRNAV
                     SVAYAAVELARKIFGHLDGRTAMLLGAGKMGELVGRHLVKNGVGRLWVCSRTYTHAVL
                     RAESLGGAAVHWEESKRKLREVDIVVSCTGAPGLVLTKPDVAASLRGRKIGPLFLIDI
                     AVPRDIDPQTNDLDNVYVYDIDALQGVIDTNLEQRRQAAERAKRMISSEVDAFVRWME
                     TQEITPLIVALRETMMGVGRLEVERFRSRLGPLDERQQQTVEELARAIIQKILHRPIR
                     RLRSSVERGDVLECSSLYREIFGIDPSADGNLDGVGPDSGTMSGPRRLLNGGKKGR"
     gene            7257..8195
                     /gene="hemC"
                     /locus_tag="VD788_08910"
     CDS             7257..8195
                     /gene="hemC"
                     /locus_tag="VD788_08910"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HXV76428.1"
                     /translation="MRELVLGTRGSALALWQARHVASALRAIEPGRRIVERIIKTAGD
                     SQQIAPLGRSDVGVFVRRIEQALLAREIDLAVHSMKDLPTRQPDGLTIASVPPRHDPR
                     DALLTIDGAQLDELPAGTLIGTGSLRRRTQLLHARPDLGTAPLRGNVDTRLAKLVAGE
                     FGAVVLAIAGIERLGLDRVPYRKLPVEVCLPAVGQGALAIETRTEDGEVRELVGALDD
                     PPSRLAVEAERAFLHELGGGCLAPATSYATIRDGRIDLTAVVGDADGIELLRDSETGA
                     STAAREIGVRLARRMKRAGADRLLELSRQAAGDERR"
     gene            8192..8974
                     /locus_tag="VD788_08915"
     CDS             8192..8974
                     /locus_tag="VD788_08915"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HXV76429.1"
                     /translation="MTPLGRPLYERVILITRPEPRNAELKRKLEALGARVEARPTIAL
                     EAPADFGPPERALRRLDDYDWLLFTSPSGVRFFLLLAQRFLGKLPRIRGSIAAVGPAT
                     GSALRAAGLRPAVVAGRSSAEGLAEAVEGRLRRGHRVLQVRPEEAREVLPEALRAAGA
                     IVDSVPFYRNVAARDVPRIARDIRRELFDAIVFTSPSTLLRLLEGAAREGIEIVDAMR
                     RTKLVAIGEITATALADAGLTTHAVAEKPSDDGIAQAVTGLF"
     gene            9041..10141
                     /gene="gcvT"
                     /locus_tag="VD788_08920"
     CDS             9041..10141
                     /gene="gcvT"
                     /locus_tag="VD788_08920"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HXV76430.1"
                     /translation="MQSTEAKRTPMYAAHLAAGARMVAFAGWQMPVQYRGVIEEHTAV
                     RTRVGLFDVSHMGEVRVRGRQALDCLQHLTCNDVARLRPGRAQYTALTTVDGCFVDDI
                     LVYRLDETDYLAVINAANATKDVDWIRQHAGAFDAEVVDESDRWCQLAVQGPAAAATV
                     AELSAAPLEPIRYYGFVRTELAGERVILSRTGYTGEDGFEIYADWSAGDRLWRAVLDA
                     GAVHGILPVGLAARDTLRLEAKMALYGNEIDDTTTVYEADLGWIVKLDKGEFIGRRAL
                     VDQRASGLRRKLVGFELGGRAIARNGFTVLAGDEPVGRVTSGTFAPHLRKSIGLAYVP
                     IERTEIGSELSIDVRGRRETAKVVATPFYKRQ"
     gene            10153..10536
                     /gene="gcvH"
                     /locus_tag="VD788_08925"
     CDS             10153..10536
                     /gene="gcvH"
                     /locus_tag="VD788_08925"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HXV76431.1"
                     /translation="MYPTDRRYSKEHEWIRVDDRVGVIGITDFAQDQLGDIVFVELPE
                     EGKAVRAGDVLGTIESVKAVSEIFSPVSGRVSAANSELDLRPELLNADPHGEGWYCKI
                     ELDDPEQLDRLMDAGGYEEFAQQGS"
     gene            complement(10552..11178)
                     /locus_tag="VD788_08930"
     CDS             complement(10552..11178)
                     /locus_tag="VD788_08930"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXV76432.1"
                     /translation="MPNEIDLSSMILVLASGSPRRLELLRGMGLAPEVVPSGLPERFE
                     PGEPPRALVRRLAEAKGRDVLPRLRRRSVVLAADTEVTLDGRVLGKPRDADHAVEMLL
                     ALCGREHEVLTGVFLVRSDDGRSVCEVESTRVAFRRYDRATIERYVATGEPMDKAGAY
                     AIQGEGSRLVRSVVGSWSNVVGLPCERLGPWLERIGLSLAALRRLRAD"
     gene            11288..11467
                     /locus_tag="VD788_08935"
     CDS             11288..11467
                     /locus_tag="VD788_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76433.1"
                     /translation="MAIVRNARMRSLAIGVGLLLGAAPGATQERTTSLVMSGEPPSLF
                     LLYTGDVIGYIEPCG"
BASE COUNT         1726 a         3968 c         4065 g         1875 t
ORIGIN      
        1 gcggagatct acgtcaccga aacgctgtgg cccgacttcc ggcgccggca tctgttccgc
       61 gcgttgctgg acttccagcg gcgcgagcgt cgttacggag ggctggggca gcccgacgac
      121 ccggccgcgg tcgtccgagg ctgaatgaag cggcttgtca ctgccgcggt gctcgtaccg
      181 ctgctgtacg gcgcggtgaa gctcgccccg ccgtgggtct tcttcggcgt cgcggtgctc
      241 gcgatcggcg gttcctgctt cgagtgctac cggatcatgc gcacctgcgg cgccgcgccg
      301 atggtgtggc ccggcgtggc ggcggcggcg gcgacggtct ggttgtacgg cgacctgccg
      361 ccgcatctgc cgccgagcct gccgctggtc gcactcgtcg tcgtcgtcac gccggtcgcg
      421 atctggaccc gccgcgcgcc cgacgcggtg ctccggacga tcgtcgacac gatcttcccg
      481 gtcgcgttcg tcgggctgac gatgggctac gtcgttgcgc tgcgtgcgct acccgacccc
      541 gacggcgagg acctggtcac gctgctgttc atctgtctga tcctggccga cacggccgcg
      601 tactacgtcg gcacgcggct cgggcggcac aagatgagcc cgcagatctc acccaacaag
      661 agctgggaag gcttcgccgg agcgatggcg ggcgcgatcg tgggaaccgc gctggcccac
      721 agctggttct acacgcgcct cccgctgtcc cacgtcgtgc cgctggggct gttgctgggc
      781 atcaccggcg cactgggcga cctggccgag agcgtcgtca agcgctcgtg ccacgtgaag
      841 gattcctcgg cgatcttccc cggacacggc ggcatcttcg accgcaccga caatctgctg
      901 atcgcggcgc ccgtccttta ctattactac ctgtggctgt tacccggagc cgtcggatga
      961 cgagcaaaag acttgccgta ctcggatcga ccggatcgat cggcgtcagc acgctcgacg
     1021 tcgtcgccgc ccatcccggt cggttcgaca tcgtcgcgct ggccgcgggc gacaatctcg
     1081 agcggatcga acgacagatc cgtcgcttcg aacccgagtg cgtcagcgtg cgcgatgccg
     1141 aggtcggtgc cgagctgcgc cggcggatcg gccgagcctg tcgcatcgtg cacggcctcg
     1201 acggtgcggt caccgtcgcg acctggccag gcgtcgacat gctcgtcagc gcgatcgtcg
     1261 gagcggtcgg actgatcccg acccacgagg cgctcgggat cggacgcgac gtggcgttgg
     1321 ccaacaaaga gtcgctggtc gtggccggcg cgcacatgac gcggcaggcc gaggagaacg
     1381 gcgccgcgat cctgccggtc gacagcgagc acaacgcgtt gcaccagtgc ctccgcggcg
     1441 agcgactggc ggagatccgc cacctgtggc tgaccgcgtc gggcgggccg ttccgcggcc
     1501 tgacggcgca gcagctcgcg caggtcgatc gagcgcaggc gctggcgcac ccgacgtgga
     1561 agatgggccc caagatcacc atcgactccg cgagcctgat gaacaagggg ctcgaggtga
     1621 tcgaggcgcg ctggttgttc ggcttgccac ccgaccggat ccgggtggtc gttcatccga
     1681 agagcgtcgt acactccctg gtggagttcg tcgacggctc gttcaaggct cagctcggcg
     1741 tcaccgacat gcggcacccg atccagtacg cgttgagcta tccggaacga tgggaatcga
     1801 cgctgccgcc gttcgatccc gtggctgccg gaccgctcga gttcgagcac cccgacttcg
     1861 agcggtttcc ctgtctggcg ctggcctttc gcgctctcga catcggtggc gcggcgccgg
     1921 ccgtagtcaa cgccgccaac gaggtcgcgg tccaggcgtt cctcgacgag cgcatcgcct
     1981 tcgtcgacat cccgcgtgcc gtgtcggcgg cgctcgagcg tcacgcgggc gcccgttcgg
     2041 actcgctcga ggagctgatc gcggtcgacc gcgcggcgcg gcgcagcacg gcggagtgga
     2101 tcggccaggg ggtcggttcg cgatgatcaa gctgctcgaa ttcgtcctcg ccttcgtgct
     2161 ggtgctggcg ccgttggtct tcatccacga gctcggccac ttcctggcgg cgaaggcgtt
     2221 tcgtgtcggc gtctcggtct tttcgctcgg attcgggccg cggttggccg gctttcgccg
     2281 ccgggagacc gactaccgca tctcgctgat cccgctcggc ggctacgtgc ggatgatcgg
     2341 cgacgagtcc gacgagcacc gcagcggcgc gagcaacgag ttcctgtcca agccgcggtg
     2401 gcagcggttc atcgtcttca tcgccggcgc ggcgttcaac atcgtgctgg cgttcgtcgt
     2461 gatgtggttc tatttcgcca ccttcggcaa gatcgacgcg ccgtaccccg aggtctacgc
     2521 cgtggcggag ggctccgagg ccgaggcgtc cggcgtccgg cgcggtgaca agctggtcga
     2581 gatctccggc aacaacgtcc agggcacgcg cgactttctc taccactaca acgtcgagat
     2641 cgcgttggcg ccggaaacgt tgaagacgat cgtcgtcgag cgcgacggcg agcgcgtccc
     2701 gctgcggttg aacaccgggc tcgacgagaa gctggggatc ggcgaccccg gttggggcgt
     2761 cggtcgcggc ggcaaccccg tcatcggcga ggtgttcgcc gacggcgcgg ccgccgaggc
     2821 cggtctgcag ccgggcgatc gcatcgtcgc cgccggcagc aagacgtcga tcaccgggct
     2881 cgagctgcag gtgttgatcg aagccagcgt cggccgggag gtgcggctgc tgatcgagcg
     2941 cgacggccgc cggctacccg ccgtcgtgcg cccgcgcgcc accgaggacg gcggcaagat
     3001 cggcatctcg ttcgtcaccg aaccgctcga gcccgtcccg ctcgacgtcg gtcaagcggc
     3061 ggtcgagtcg tttcgctgga atctggacct gtcgaagacg ctgttcgtcg tgctcaagcg
     3121 gatggtgacc accgagctct cgatcaagac gatgtcggga ccggtcggca tcgctcaggt
     3181 cgcgcgtcag gcgctgctca ccggcgtcga caccttcctc tatctgctcg cctttttcag
     3241 cctacagctc ggcatcctca atctgatgcc gattccgatt ctcgacggcg gacacatcct
     3301 gatcctgatc gtcgaaggcg tgatgcggcg aaatctgtcg gaggcggtca aggagcgcgt
     3361 gatgcaggcc ggtctggtct tcctgatcgc gttcatgggc gtcgtcatct tcctcgacgt
     3421 ggtcaagatc ctctgacgtc cgcatgcgga cggcgcggcg gcgctcgcgt ccgccggcgg
     3481 ccgccgcgcg cgcgcgatcg acgaaaacga cgtggttcct cctggcacgg gggttgctgt
     3541 tctccccggc gctcggcgtt cgccccgcgc cgcgcggccc gtggcatcga gccaacgcgg
     3601 cgccggctcc gacggagcgt tctcggggca aggcggtagc caccgccagg aggaagttcg
     3661 atgaagatcg cgaagaaccc gctcgtccgc cgcgccgccg ccgtgttggc cgtggtgtcg
     3721 ctggctgcgg ccgtcgccgc cgcgccgtcg ggctcggccg attcgacgga gaccccgcgt
     3781 ccaccggcgg ccaagatcga tctgaaccag gcgacccaag agcaactcga gtcgatcccg
     3841 gggatcggtc cggcgctcgc gaagcggatc ctcgactttc gcaccaagga gggcaagttc
     3901 ctccgagtcg acgacctgct gaaggtccgg ggtatcggcg agaagtcgct cgagaagctg
     3961 cgtccgtacg tcacggtcga gccccgggcc tgagccgctg gcgttctccg cgggcgctcg
     4021 cgcgagaacg agcgcccgcg gaggctcacg caccgtgaag cgcaaaggct acacgctggt
     4081 cgagctgctc gtcgcgctgg cgatcgtcgc gatcatcgcg tcgaccgcaa ccgtcgcgct
     4141 cgccgaccgg ttgggcggac atgccgcgac ggccggcgcg cgcgagctgt cggcgacgct
     4201 gcagtcgctc cgcttccgcg cggtggcgac caaccggcat cacggtctgt acttcgagcg
     4261 cgacgcgacc ggctggttct ggttcgtggt ccgcgacgag aacggcaacg gtcttcgcac
     4321 gcgtgagatc ctcgacggcg tcgatcgacg gctctcgggg ccgcaccgcc tgcgcgacag
     4381 gtcgcgcggc atcgatctcg gcttcccggc cgtgacgatc ccggcgctgc cgcctcgcac
     4441 cggctcgatc gacgatctcg acgatccggt caagttcggc cgggccgatc tggtgtcgtt
     4501 cgcgccgctc gggacggcgt ccggcggcac gctctacctc accgacggcc gctacgcgct
     4561 gcgcgcggtc gtgttgttcg ggccgacggc gcgggtgcgt gtttggcgct tcgacacgcg
     4621 ggagggtcga tggagactct gaagtccgat cgacgcatcg acgtcttccg ctcgaccgat
     4681 tcgttcgcgg tcgaggctta ccgggccgcc acccagatcc gctcgaacgt cggcgccggc
     4741 ctggccgagg agatccggcg aaccgcggtc gccgccggcg gcgcgatgat cgccgcctca
     4801 tcgtatccgg ccggtgccgg cgccgagcgg gtccacctgg aacgcgctcg ggcggcattg
     4861 ctcgagagcc gctactacct gcacttggcc cggcgcttcg gctggctcga cgccaagagc
     4921 tatcgcgcgc tgaccctgcg ccaggacgcc gcggtgcgcg gcctcgacgg cctgatcggc
     4981 catggcagcc agcatgcgag acgggagaga tgagcccgcc gtcgtccagt tcccggcttg
     5041 agaagccccg gccgcggtgg tagctttccg acggggggta atcgggccgg ttcagccgct
     5101 ggcaacccat ggatctcacg tcggcaagcg ctctcctgcc ggtcacggtc atcggctaca
     5161 tcgtcgccgt cgtcacggtg acgatccgaa cgatctatcg cgcggcggcg gcgcggcggc
     5221 tgtcctccgc gatgttcgtc ctcacctggg ttctgcacag cgcgacggtc gtgcagctcg
     5281 gcctggccaa cggacgtctg ccgctggcca ccggcgccga atacctgctc gtgctcggct
     5341 gggtcgtgct gacgctgcac ctctacgttt ggttcaagct gagcatggag gtggccggcg
     5401 tcgtattgcc gccattggcc ggcgcgatga cgttcggcgc gatgcagctg atcggcgtgg
     5461 ccacacctgc cgcggtcacc cgcgacgcgt ggttcctgtt ccacaccacc gtgtcgacac
     5521 tcggcatcgc gatcctgagt gtcgcgttcg cgatgagcgt cctgtatctg ctgcaagatc
     5581 gcgcgctcaa gtcgaaaaag acgctggcga tgctcgagcg gctgccgtcg ttgcagaagg
     5641 ccgaccagat cggtttccgc tcgctgacga tcggcttcgt cctgctcacg ctcggcatcg
     5701 cgaccggtgt cgtggtcaac gccgaggtct atcagcgcct cgtcgtgctg ggcccgaagc
     5761 aggtcttccc gctgctggcc tggctgatct ttgccggcgt gctgatcgcc cgtccgacga
     5821 tcggttttcg cggccgcaag tcggcctacc ttacgatcac gggattcgcc ttcggactga
     5881 tgaccgtgat cggcatgacg ctgtagacga tgctggtact ggtcggatcg aatcatcgca
     5941 gcgcgccgct ggcggtgcgc gagcggatga gcttcccgct ggagaagatc tccgaggcgc
     6001 atacgcgact gatgcaactc gacggcgttc gcgagggcat gatcctgtcg acctgcaatc
     6061 gcgtcgagat cctggcgcac accgatgccg acggcgcgca gtcgttgcag cgttttctcg
     6121 ccgatcaaca tcggctatcg ctcgacgagg tcgagcgcta cacctatcga ctggccgacc
     6181 gcgacgcgat ccgccacctg ttcagcgtcg cgagcgggct cgattcgatg atcatcggcg
     6241 agccgcagat cctcgggcag gtcaagcaag cctaccagat ggcgaaggac ctgcggacgc
     6301 tgggcccggc gctcgaacga ttgctgcagc acgggctggc gacggccaag cgggtccgta
     6361 gcgagacggg tatctcgcga aacgcggtgt cggtggccta cgctgcggtc gagctcgcac
     6421 ggaagatctt cggtcatctc gacggccgca cggcgatgct gctcggcgcg ggcaagatgg
     6481 gcgagctcgt cggccggcac ctggtcaaga acggcgtcgg tcggctgtgg gtctgcagcc
     6541 gcacctacac ccacgccgtg ctgcgcgccg aatcgctcgg cggagccgcc gtgcattggg
     6601 aggaatcgaa gcgcaagctg cgcgaggtgg acatcgtcgt cagctgcacc ggcgcgcccg
     6661 gactggtgct gaccaagccg gacgtcgccg cctcgctgcg cggccgcaag atcggcccgt
     6721 tgttcctgat cgacatcgcg gtgccgcgcg acatcgaccc gcagaccaac gatctcgaca
     6781 acgtctacgt ctacgacatc gacgccctgc agggagtgat cgacaccaat ctggagcaac
     6841 ggcggcaggc cgccgaacgc gccaagcgga tgatctcgag cgaggtcgat gccttcgtgc
     6901 gatggatgga aacgcaggag atcaccccgt tgatcgtcgc gttgcgcgag acgatgatgg
     6961 gtgtcggacg actggaggtc gagcgcttcc gctcgcgctt ggggccgctc gacgaacggc
     7021 aacagcagac cgtcgaagag ctggcgcggg ccatcatcca gaagatcctg catcgtccga
     7081 tccggcggtt gcgttcgtcg gtcgaacgcg gcgacgtgct cgagtgctcg tcgctctatc
     7141 gcgagatctt cgggatcgac ccgtcggccg acgggaatct ggacggcgtg ggccccgatt
     7201 cgggtacgat gtccgggccg cggcggctgc tgaacggtgg caagaaaggt cgctgagtgc
     7261 gggaacttgt cctcggtacg cgtggcagcg cgctcgcgct gtggcaggcg cggcacgtgg
     7321 ccagcgcgct gcgtgccatc gagcccggcc gccgcatcgt cgagcgcatc atcaagaccg
     7381 cgggcgactc gcagcagatc gcgccgctcg gccggtcgga cgtgggtgtc ttcgtccgcc
     7441 gcatcgagca ggcgttgctc gcccgcgaga tcgatctcgc ggtgcacagc atgaaagacc
     7501 tgccgacgcg gcagcccgac gggctgacga tcgcctccgt tccgccgcgc cacgacccgc
     7561 gtgacgcgtt gttgacgatc gacggcgccc agctcgacga gctgcccgcc ggcacgctga
     7621 tcggaaccgg cagtcttcgt cgcaggacgc agctgctcca cgcgcgtccc gatctcggca
     7681 cggcgccctt gcgcggcaac gtcgacacgc gactggccaa gctggtcgcg ggcgagttcg
     7741 gcgcggtcgt gttggccatc gccggcatcg aacggttggg gctcgaccgt gtgccctacc
     7801 gcaagctgcc ggtcgaggtc tgcctgccgg ccgtcggcca aggcgcgctc gcaatcgaga
     7861 cgcggacgga agacggcgag gtccgcgagc tggtcggggc gctggacgac ccgccgtccc
     7921 gtctcgccgt cgaggccgaa cgggcctttc ttcacgagct cggcgggggc tgtctggccc
     7981 cggcgacctc ctacgcgacg atccgcgacg ggcgcatcga cctcaccgcg gtcgtcggcg
     8041 acgccgacgg gatcgagctg ctgcgcgact cggagacggg tgcctcgacg gcggctcgcg
     8101 agatcggtgt gcgcttggcg cgacggatga agcgcgcggg ggcggaccgc ttgctcgagc
     8161 tgtcgcgcca ggcggccggc gacgagcgtc gatgacccca ctcggtcgac cgctctacga
     8221 gcgggtcatc ctgatcaccc gtcccgaacc acgcaacgcc gagctcaaac gcaagctcga
     8281 agcgctcggc gcgcgggtcg aggcacggcc gacgatcgcg ctcgaggcac ccgccgactt
     8341 cggcccgccg gagcgcgcgc tgcggcggct cgacgactac gactggctgc tgttcaccag
     8401 cccgagcggg gtccggttct tcctgctgct cgcgcagcgt tttctcggca agctcccgcg
     8461 gatccgcggc tcgatcgcgg ccgtcgggcc ggcaacgggc tccgcgctgc gcgcggccgg
     8521 actgcgaccg gcagtggtcg ccggccgcag cagcgcggaa ggcctggccg aggcggtcga
     8581 ggggcgtctc aggcggggtc accgtgtgct acaggtccga cccgaggagg cccgcgaggt
     8641 gctgcccgaa gcgctgcggg cggcgggcgc gatcgtcgac tcggtgccgt tctatcgcaa
     8701 cgtcgccgcg cgcgacgtcc cgcgcatcgc acgcgacatc cgccgcgagc tgttcgacgc
     8761 gatcgtcttc acttcgccct cgacgctgct gcggttgctc gagggggcgg cgcgcgaggg
     8821 gatcgagatc gtcgacgcga tgcgccggac gaagctcgtc gccatcggtg agatcacggc
     8881 gacggccttg gccgacgccg ggttgacgac ccacgccgtc gcggagaaac cgtcggacga
     8941 cggaatcgcg caggcggtga ccggcctgtt ctgaacgatg tgttaccatc gctggctgcg
     9001 cagttcgagc cggcccgaca gcgccgcgcg ggggagtcgg ttgcagtcca ccgaggccaa
     9061 acgaacaccg atgtacgcgg cgcacctggc cgcgggcgcg cggatggtcg cgttcgccgg
     9121 ctggcagatg ccggtgcagt accgcggcgt gatcgaggaa cacaccgccg tacgtacgcg
     9181 ggtcggcttg ttcgacgtca gtcatatggg cgaagtccgg gtgcgcggcc ggcaggcgct
     9241 cgactgcctg caacacctca cctgcaacga cgtggcccgg ctgcggccgg gacgcgcgca
     9301 gtacactgcg ctgacgaccg tcgacgggtg tttcgtcgac gacatactgg tctaccggct
     9361 ggacgagacc gactacctgg cggtgatcaa cgccgccaac gcgaccaagg acgtcgactg
     9421 gatccgccag cacgccggcg cgttcgacgc cgaggtcgtc gacgagtcgg accgctggtg
     9481 tcagctcgcg gtccaaggcc ccgccgccgc ggcgaccgtc gccgagctgt cggccgctcc
     9541 gctcgagccg atccgctact acggcttcgt tcgcaccgag ctcgccggcg agcgcgtgat
     9601 cctgtcgcgc accggctaca cgggtgagga cggcttcgag atctacgccg actggtcggc
     9661 cggcgacagg ctctggcgtg ccgtgctcga cgccggcgcc gtccacggaa tcctgccggt
     9721 cgggttggcc gcgcgcgaca cgttgcggct cgaggccaag atggcgctgt acggcaacga
     9781 gatcgacgac acgacgaccg tctacgaggc cgatctcggc tggatcgtca agctcgacaa
     9841 gggcgagttc atcggccgcc gcgcgctggt cgatcaacgg gcgagcggtc tgcggcgcaa
     9901 gctcgtcggc ttcgagctcg ggggccgggc gatcgcgcgc aacggattca ccgtgctcgc
     9961 cggcgacgag ccggtcggtc gggtgacgag cggcacgttc gcaccccacc tgcggaagag
    10021 catcgggctg gcctacgtgc cgatcgagcg gaccgagatc ggcagcgagt tgtccatcga
    10081 cgttcgaggg cggcgcgaaa cggcgaaggt cgtcgcgacg ccgttctaca agcgacagta
    10141 ggagacgcca cgatgtaccc caccgaccgg cgctacagca aggaacacga gtggattcgc
    10201 gtcgacgacc gtgtcggcgt gatcgggatc accgacttcg cgcaagacca gttgggcgac
    10261 atcgtcttcg tcgagctgcc cgaggaagga aaggcggtgc gtgccggcga cgtgctgggc
    10321 acgatcgaat cggtcaaagc cgtctccgag atcttctcgc cggtgagcgg cagggtatcg
    10381 gccgcgaact cggaactcga cctgagaccc gaattgctca acgcggaccc gcacggtgaa
    10441 ggctggtatt gcaagatcga gctggatgat ccggagcaac tcgacagact gatggacgcg
    10501 gggggctacg aggagttcgc gcagcagggc tcctgagccg ggtcgtcgcg ctcagtcggc
    10561 gcgaagccgg cggagcgcgg ccagcgacag cccgatgcgt tcgagccagg gaccgagtcg
    10621 ctcgcacggc agaccgacca cgttgctcca cgatccgacg acactgcgca ccaaccgcga
    10681 gccctcaccc tggatcgcgt aggccccggc cttgtccatc ggctcgccgg ttgcgacgta
    10741 gcgttcgatc gtcgcccggt cgtagcgccg gaacgcgacc cgcgtgcttt ccacctcgca
    10801 gaccgatctc ccgtcgtcgc tccggacgag gaacaccccg gtgagcacct cgtgctcgcg
    10861 cccgcacagc gcgagcagca tctcgaccgc atgatcggcg tcgcgcggct tgcccagcac
    10921 gcgtccgtcg agcgtcacct cggtgtcggc agcaagcacc accgaccgcc gccggagccg
    10981 gggcagcacg tcgcgcccct tggcctcggc cagacgccgg acgagcgctc gcggcggttc
    11041 acccggctcg aaccgctccg gcagaccgga aggaacgacc tcgggggcga gccccatccc
    11101 gcgcagcagc tcgagccgac gcggcgagcc cgacgccagc accaggatca tcgagctcag
    11161 gtcgatctcg ttgggcacgc gccacttata cacacttttt acgtcgaggt cgcccgccgg
    11221 gccgccggcg catgtagatt actctgtcgt cccggcgtcg cggcgtcggg cgggaggtgg
    11281 taacgagatg gcgatcgttc ggaacgcgcg gatgcggtcg ctcgcgatcg gcgtcggact
    11341 gttgctgggc gccgcaccgg gcgcgacgca ggagcgcacg accagcctgg tgatgtcggg
    11401 cgagccgccg tcgctgttcc tgctctacac cggggacgtg atcggctaca tcgaaccctg
    11461 cgggtgaaag aagaaccccg cggggggcct ggcccggagg gcttgggtca tcgatcaagt
    11521 ccgaacgaag ttccccgaca cgcccgtttt ggtgttggac agcggcaact tctcagatgc
    11581 gccgacgccg aacggactca cacgcacgcg ggcgttgctc gaggggatgg agac
//
LOCUS       DASPGN010000307         5830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_991155, whole genome shotgun sequence.
ACCESSION   DASPGN010000307 DASPGN010000000
VERSION     DASPGN010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5830
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991155"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1818)
                     /locus_tag="VD788_08940"
     CDS             complement(<1..1818)
                     /locus_tag="VD788_08940"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HXV76434.1"
                     /translation="MVRRFEDILERVSAFDPGVDQDLLRKAYVFSAREHRHQKRRSGE
                     PYLTHPIEVAYILADLELDAASIVSGLLHDVVEDTLTNIENVSDHFGEDVAHIVAGVT
                     KISKLKFSNSQEAEAENLRKMILAMVDDIRVILVKLADRLHNMRTLEHLPPPKQQRIA
                     RETKEIYAPIANRLGIGRIKHELEDLSFMYLEPEAHAELARALASRRKVSDRFIDEIR
                     QRLEGAMQSAGIDCEITGRLKSTYSIHKKMKIQKIGVDEVYDYIAFRILTTSIKDCYG
                     ALGIVHSIWRPVPGRIKDYIAMPKPNMYQSLHTSVMTDKGHPFEVQIRTHEMHRIAEE
                     GIAAHWQYKEGGRLSDHDAEKMTWLRQILDWQKDLKDPRDFMELVKIDLFPEEVYAFT
                     PKGKVLSFRAGATPLDFAYAIHTEVGHQCTGAKVNGRIVPLRYELKNGDIVEILTNPN
                     RHPSRDWLSLARTSRARGKIRAWLNANERERSVALGRELTDKHFRRFKVTLKEFDTDS
                     VLEPALAKLGLAQLDDFYAAVGYGKLTPQSLLTLLVPESDLQEKKEGVVERVVKRALR
                     RSDRGISVRGLDDVMVTLAKCCNPVRGEEIIGYISRGRGV"
     gene            complement(1928..3136)
                     /gene="secF"
                     /locus_tag="VD788_08945"
     CDS             complement(1928..3136)
                     /gene="secF"
                     /locus_tag="VD788_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXV76435.1"
                     /translation="MWQILKQSNIDFMGQGRVLLPVSLALVALSVAVLVFNGFNLGIE
                     FTGGTELHVKYAETPRVGEIRGQLSAGGFGGAVVTTIGDAEDNEIYIRLATGGEQQGS
                     DPTTAVLNILRGIPAGDQRSTIDLNIDDERTLAGLLAGAPGLSRARAEEIAAELLQWR
                     REGNIFSSFQQLSAFSGLTPEGIDHLEANSEIGPLALRSQSFIGPAVGRELVEKALIA
                     VVGSLIGMLIYIWIRFQLQWGMAAVAALVHDTLITLGLFSLFDQEMSLPVVAAFLTLI
                     GYSVNDTVVVFDRIRENLAAFRSKALADTVNLSINQTLSRTIITSGLTWLVVVCLLLF
                     GGPALRPFSFVLTVGVAVGTYSSIFIAAPLVVLWQAARDRRKTAAEGGVASDEAPARK
                     AKKIRRTKTI"
     gene            complement(3161..4792)
                     /gene="secD"
                     /locus_tag="VD788_08950"
     CDS             complement(3161..4792)
                     /gene="secD"
                     /locus_tag="VD788_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXV76436.1"
                     /translation="MSNSPLFWKFALILAVVAAAAAFCFPPQDRINLGLDLKGGAHIL
                     MQVDTDSAIKFEMDRLQNSLGQRFKSEGIGYEAILPAGEATLEIRGTSEGQRAQVRDE
                     LNQFVGGWDHESVGQGTWRLTMPGAYRSQVEVEAVDTTLATIRRRIDSLGVSDPTIQK
                     SGIDGRRILVQLPGIEDPTRVKELLKDPAVLEWKEVSYPPGSDFENYFPPASQEAVIA
                     QFGGELPADTELFPQRIEQPDGTTIEAWWPLKRVSTVAGRDLRDARRSADEWGDPAVS
                     FELTQEAGKRFEIATRENLGRKMAIVLGGADSKTVISAPVIEGIIRDQGVIRGGFDVQ
                     RADDLAIKLRSGAIPTDVTILEERTVGPSLGRDSIRSGLAAGLAGFLGVMLFMLAYYR
                     LSGINAVIALALNVLLVFGALGALPFLFSAAGIRATLTLPGIAGLILVIGMAVDSNVL
                     VFERIREELQIGKTVRSAVDQGFAKAFSTILDCNVTTVVAAIFLSVYGTGPVRGFAVT
                     LIIGLIASMFTAVFVSRQLFELVLLKLSPDRTLSI"
     gene            complement(4808..5134)
                     /gene="yajC"
                     /locus_tag="VD788_08955"
     CDS             complement(4808..5134)
                     /gene="yajC"
                     /locus_tag="VD788_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXV76437.1"
                     /translation="MIDPLQTLLAVAPPTEEGAPSLFMTFLPMIMIFVVFYFLMIAPA
                     RRKQKKHAEMLQNLKNGDRVITNGGIHATVVGVTDNVVQLRVADKVKIDVSRQAIAAL
                     QSPTDE"
     gene            complement(5258..>5830)
                     /locus_tag="VD788_08960"
     CDS             complement(5258..>5830)
                     /locus_tag="VD788_08960"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HXV76438.1"
                     /translation="RDADRDPPIVPLGDGAIALRTDGSRRVAAVRTADCVPLLVAERS
                     GHAVAAVHAGWRGTAAGIATRCVSRLSRAGVRPRRLVAAIGPAVRACCYEVSADVVAV
                     VSRASGVDAARVSHDGIRGRPMLDLAGAVAAQLVSAGIAAEAVSVAPWCTACSEELFF
                     SYRRDGATAGRMMAVIGWRSDCGASSGTVA"
BASE COUNT          964 a         1944 c         1951 g          971 t
ORIGIN      
        1 gaccccccgg ccgcggctga tgtagccgat gatctcctca ccgcggaccg ggttgcagca
       61 cttggccagc gtgaccatca cgtcgtcgag gccgcgaacc gagattccgc gatcgctgcg
      121 gcgcagcgcc cgcttgacga cccgctcgac gaccccttcc ttcttctctt gcagatcgct
      181 ctcggggacc aacaacgtga gcaacgattg cggggtgagc ttgccgtagc cgacggcggc
      241 gtagaaatcg tcgagctgcg ccaggcccag cttggccagc gccggctcga gcacggagtc
      301 ggtgtcgaac tccttcagcg tcaccttgaa ccggcgaaag tgcttgtcgg tcaactcgcg
      361 cccgagggcg accgaccttt cgcgctcgtt ggcgttgagc cacgcgcgga tcttgccgcg
      421 ggcgcgcgag gtccgagcca acgacaacca gtcgcggctg ggatgtcggt tcgggttggt
      481 caggatctcg acgatgtcgc cgttcttcag ctcgtagcgc agcggcacga tccggccgtt
      541 gaccttcgcc ccggtgcact ggtgtccgac ctcggtgtgg atcgcgtagg cgaagtcgag
      601 cggggtcgcg ccggcacgga acgacagcac cttgcccttc ggcgtgaagg cgtagacctc
      661 ctcgggaaac aggtcgatct tgaccagttc catgaaatcg cgggggtcct tcaggtcctt
      721 ctgccagtcg aggatctggc gcagccaggt catcttctcg gcgtcgtggt ccgacagcct
      781 tccgccttcc ttgtactgcc agtgcgccgc gatcccctcc tcggcgatgc ggtgcatttc
      841 gtgggtgcgg atctgcacct cgaacgggtg gcccttgtcc gtcatcaccg aggtatggag
      901 cgactggtac atgttcggct tcggcatcgc gatgtagtcc ttgatccggc ccgggacggg
      961 tctccagatc gagtggacga tccccagcgc accgtaacaa tccttgatcg aagtggtcag
     1021 gatgcggaac gcgatgtagt cgtagacctc gtcgacaccg atcttctgga ttttcatctt
     1081 tttgtggatc gagtaggtcg acttgagccg cccggtgatc tcgcagtcga tcccagccga
     1141 ttgcatcgcg ccctcgagac gctggcgaat ctcgtcgatg aagcggtccg agaccttgcg
     1201 ccgcgaggcc agcgctcgag ccagctcggc gtgggcctcc ggttcgaggt acatgaacga
     1261 caggtcctcg agctcgtgct tgatccggcc gattcccagg cgattcgcga tcggcgcgta
     1321 aatctccttc gtctcccggg cgatgcgctg ctgcttgggc ggcggcaggt gctcgagcgt
     1381 ccgcatgttg tgcagccggt cggccagttt gaccaggatc acgcggatgt cgtcgaccat
     1441 cgccaggatc atcttgcgca gattctccgc ctcggcttct tgcgagttcg agaacttgag
     1501 cttcgagatc ttggtgacgc cggcgacgat gtgggccacg tcttcgccga agtggtcgga
     1561 gacgttctcg atgttggtca gcgtgtcctc gacgacgtcg tggagcagtc cggagacgat
     1621 gctggccgca tccagctcga ggtcggcgag gatgtaggcc acctcgatcg ggtgcgtcag
     1681 gtacggctcc cccgatctgc gcttctggtg acgatgctcg cgcgccgaga acacgtaggc
     1741 cttgcgcagc aggtcctggt cgacgccggg gtcgaacgcg ctgacgcgct cgaggatgtc
     1801 ttcgaatcgc cggaccattt cgacggacct acgcgagaga cggaccgcga cccggaacgg
     1861 ggcgcggcgc tcccatatcg ggaattataa ggccggcctc cccgcggagg ccgacgacga
     1921 ccggcgctca gatggtcttg gtccggcgga tcttcttggc cttgcgggcg ggagcctcgt
     1981 cgctcgccac gccgccctcg gcggccgtct tccggcgatc gcgcgctgcc tgccacagga
     2041 ccacgagcgg tgcggcgatg aaaatcgacg agtaggtgcc gacggccacg ccgacggtca
     2101 gcacgaagga gaacggccgc aacgccggcc cgccgaacaa cagcagacag acgaccacga
     2161 gccaggtcaa tccggaggtg atgatcgtcc ggctcagcgt ctggttgatc gacaggttga
     2221 ccgtgtcggc cagcgccttg ctgcgaaagg cggcgaggtt ctcgcgaatc cggtcgaaca
     2281 cgacgacggt gtcgttgacc gagtaaccga tcaaggtcag gaaggcggcc accaccggca
     2341 ggctcatttc ctggtcgaac aaggaaaaca agcccaacgt gatcagtgtg tcgtggacca
     2401 gcgccgcgac cgcggccatg ccccattgga gctggaagcg gatccagatg tagatcagca
     2461 tcccgatcag cgaaccgacg accgcgatca gcgccttttc caccagctcg cggccgaccg
     2521 cggggccgat gaacgattgg ctgcgcagcg cgagcggtcc gatctcgcta ttggcctcga
     2581 ggtggtcgat tccctccggc gtcaggccgg agaaggcgga cagttgctga aagctcgaga
     2641 agatgttgcc ttcacgccgc cactgcagca gttcggccgc gatctcctcg gcacgcgccc
     2701 ggctcagacc gggcgccccg gcgagcagac cggccagcgt ccgttcgtcg tcgatgttca
     2761 ggtcgatggt cgagcgttgg tcccccgcgg ggatcccgcg caggatgttc aacaccgcgg
     2821 tcgtcgggtc gctcccctgc tgctcgccac cggtggccag tcggatgtag atctcgttgt
     2881 cctcggcgtc gccgatcgtc gtgacgacgg ccccgccgaa gccgcccgca gagagctgac
     2941 cgcggatctc gccgacgcgc ggggtttcgg cgtacttgac gtgcagctcg gtgccgccgg
     3001 tgaactcgat gccgagattg aacccgttga acaccagcac cgcgaccgac agggcgacca
     3061 gcgcgagcga gacgggcagc agcacgcgcc cttggcccat gaaatcgatg ttcgactgct
     3121 tgagaatctg ccacacgatc ggtacctcgt cccgctcggg tcagatgctg agcgtccggt
     3181 cgggactgag cttgaggagt acgagctcga acaactgacg ggagacgaag accgcggtga
     3241 acatcgacgc gatcaggccg atgatcagcg tcacggcgaa gccacgcacg ggaccggtgc
     3301 cgtagacgct gaggaagatc gcggcgacga cggtggtcac gttgcagtcg aggatggtgc
     3361 tgaacgcctt ggcgaagccc tgatcgaccg cggaacggac ggtcttgccg atctgcagct
     3421 cttcacggat gcgctcgaag accagcacgt tgctgtcgac ggccatgccg atgacgagga
     3481 tcaggccggc gatgccgggg agcgtcagcg tggcgcggat gcccgcagcg gaaaagagga
     3541 acggcagcgc accgagcgcg ccgaagacga gcaacacgtt gagcgcgagg gcgatgacgg
     3601 cgttgatccc ggacagccgg tagtaggcga gcatgaacag catcacgccg aggaagccgg
     3661 cgaggccggc cgccagaccg gaccggatcg agtcgcgtcc gagcgaggga ccgacggtcc
     3721 gctcctcgag gatcgtgacg tcggtcggga tcgcgccgga ccgcagcttg atcgccaggt
     3781 cgtcggcgcg ctggacgtcg aacccgccac ggatcacccc ttgatcgcgg atgatgccct
     3841 cgatgaccgg cgccgagatg acggtcttgc tgtccgcccc acccaacacg atcgccatct
     3901 tgcgtccgag gttctcgcgg gtcgcgatct cgaaccgctt gccggcctcc tgtgtcagct
     3961 cgaagctcac cgccgggtca ccccactcgt ccgcgctccg ccgcgcgtcg cgcaggtcgc
     4021 gcccggcgac cgtcgacacg cgcttgagcg gccaccacgc ctcgatggtg gtgccgtccg
     4081 gctgctcgat ccgctgcgga aacagctccg tgtcggcggg cagctcgccg ccgaactggg
     4141 cgatgacggc ctcctggctg gcggggggaa aatagttctc gaaatccgac ccagggggat
     4201 agctcacttc cttccactcg agcaccgccg ggtccttcaa cagctccttg acccgggtcg
     4261 ggtcttcgat tcccggaagc tggacgagga tgcgacggcc gtcgatgccg gatttctgga
     4321 tcgtcgggtc gctgacgccg aggctgtcga tgcggcggcg gatcgtcgcc aacgtggtgt
     4381 cgacggcctc gacctcgacc tggctgcggt aggcgccggg catcgtcagc cgccaggtgc
     4441 cttgcccgac actctcgtgg tcccaaccgc cgacgaactg gttgagctcg tcgcggacct
     4501 gtgcgcgctg gccctcgctc gtcccgcgga tctcgagcgt ggcctcgcct gcgggcagga
     4561 tcgcctcgta gccgatcccc tcgctcttga accgctggcc gagcgagttc tgcaggcggt
     4621 ccatctcgaa cttgatcgcc gagtcggtgt cgacctgcat caggatgtgc gctccaccct
     4681 tgaggtcgag accgagattg atccggtcct gcggtgggaa gcagaacgcc gccgccgcgg
     4741 cgacgacggc caggatcaac gcgaatttcc agaacagcgg gctgttcgac atcgtggttc
     4801 cctcggctca ctcgtccgtc ggactctgca gcgcggcgat cgcctggcgc gatacgtcga
     4861 tcttgacctt gtccgccacc cgcagttgga cgacgttgtc ggtaacgccg acgaccgtgg
     4921 cgtggattcc cccgttggtg atgacgcgat ccccgttctt caggttttgc agcatctcgg
     4981 cgtgcttctt ctgcttccgt cgtgccgggg cgatcatcag gaagtagaag acgacgaaga
     5041 tcatgatcat cggcaggaag gtcatgaaca gcgaaggggc gccctcctcc gtcggagggg
     5101 ccaccgccag gagtgtctgc agcggatcga tcatcgcgag cttccacctc gccggaccgg
     5161 gcacgagcgc agggtcgtac gaccggtcac cccacggtcg gcccgtctct cgcgccagtt
     5221 gacgaacgga ggattctgac ccaccgtcgg ggacccgtca agcgaccgtc ccggacgacg
     5281 cgccgcaatc ggaacgccag ccgatgaccg ccatcatccg gccggccgtc gccccgtcgc
     5341 ggcggtagga gaaaaacagc tcctcgctgc aggcggtgca ccacggggcg accgagacgg
     5401 cctcggcagc gatcccggcg gagacgagct gcgccgcgac ggcgccggcc agatcgagca
     5461 tcgggcgccc ccgaatcccg tcgtgcgaca cgcgcgccgc gtcgacgccg ctcgcacggc
     5521 tgaccacggc gacgacgtcg gccgaaacct cgtagcagca cgcgcggacc gccggcccga
     5581 tcgcggcgac cagccgccgg ggccgaacgc cggcgcgcga cagccgggat acgcagcggg
     5641 tcgcgatccc ggcggccgtg ccgcgccagc cggcgtggac cgccgcgacc gcgtgcccgc
     5701 tgcgttcggc gaccagcagc gggacgcagt ccgcggtccg caccgcggca acccgacgcg
     5761 aaccgtccgt gcgcaacgcg atcgcgccat ccccgagcgg gacgatcggc ggatcgcgat
     5821 cggcgtcgcg
//
LOCUS       DASPGN010000308         2479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_406575, whole genome shotgun sequence.
ACCESSION   DASPGN010000308 DASPGN010000000
VERSION     DASPGN010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406575"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VD788_08965"
     CDS             <1..892
                     /locus_tag="VD788_08965"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXV76439.1"
                     /translation="GLQEKYGALRVSDAGIREATIVGQAIGMALRGLRPLAEIQYLDY
                     LLYALQIISDDLATLHYRTRGGQKAPVLIRTRGHRLEGIWHSGSPMAGILNLVRGIYL
                     CVPRDMTRAAGFYNTMLASDDSAVIVEVLNGYRLKEALPANLDRYRLPLGVPELLREG
                     GDVTVVTYGACCRIALDAAELLSRVGIETEVIDVQTLLPFDRVGRIVESLKKTGRVVF
                     VDEDVPGGATAYMMQQVIDQQRGFWWLDAEPKAVSAKAHRPAYGSDGDYWSKPNREQV
                     FRVVYGLMHEAHPDRFPIFY"
     gene            911..2074
                     /locus_tag="VD788_08970"
     CDS             911..2074
                     /locus_tag="VD788_08970"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXV76440.1"
                     /translation="MGERVLSRLRPGDYRLPDELVGRLLTPALVIYIDLVRDNLRRVI
                     DRAGGEVDRLRIHVKTAKIPAVFAELVAAGIRNYKCATLREARELLQVLHDRQVTASD
                     LLVAYPLREPALGQAAELARRFPATRLSVLCEDPTNLRSIPEELSVFVDVNPGMNRTG
                     VPAADEARITELARAAAGRFRGLHYYDGHVHGKDAAERRAMAHDGYGRLVGLTERLLR
                     RSLPVGEIVTSGTPAFVHALGFDPLRSFDHTVHRVSPGTVVYHDARYALELDELELTP
                     AALVWTRVVSRPADEIATCDAGSKSIAAEAGDPCALVVGHPDYRALRPSEEHLPLAVP
                     PADAPRLGAMLTLIPRHVCPTVNLAEQALLVERGRSPRVVSVSARAHDLIAGE"
     gene            2100..>2479
                     /locus_tag="VD788_08975"
     CDS             2100..>2479
                     /locus_tag="VD788_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76441.1"
                     /translation="MMLLGVCLLVAPRPEAADWEIVPFAGFRWGGEVTEIDTNRTVDF
                     DPSAVFGLTVGRYLSSESRVEGTWSRQASSLAGDGLDITLDYLHFAGVYEPHPARAST
                     AYVLVSGGVTRFDASGAVATTHLS"
BASE COUNT          349 a          821 c          893 g          416 t
ORIGIN      
        1 cggcctgcag gagaagtatg gcgcgttgcg ggtcagcgac gccgggatcc gcgaggcgac
       61 gatcgtcggg caggcgatcg gcatggcgct gcgcggtctg cggccgctgg ccgagatcca
      121 gtacctcgac tacctgctct acgcgttgca gatcatctcg gacgacctgg ccacgctgca
      181 ctaccggacg cgcggcggac aaaaggcgcc ggtgctgatt cgcacgcgcg ggcatcgcct
      241 ggagggaatc tggcattcgg ggtcgccgat ggccgggatc ctcaatctgg tgcgcggtat
      301 ctacttgtgc gtcccgcgtg acatgacacg cgcggctggt ttctacaaca cgatgctcgc
      361 ctcggacgac tcggcggtca tcgtcgaggt gctcaacgga taccggctca aggaggcgct
      421 gccggcgaac ctcgatcgct accggctgcc gctcggtgtg cccgagctgc tgcgcgaggg
      481 cggcgacgtc acggtcgtga cctacggtgc gtgttgtcgc atcgcgctcg acgcggccga
      541 gctgttgagc cgggtcggga tcgagaccga ggtgatcgac gtccagacgc tgctgccgtt
      601 cgatcgggtg ggacggatcg tcgagtcgct gaagaaaacc ggacgcgtcg tcttcgtcga
      661 cgaggacgtt ccgggcggcg caacggcgta catgatgcag caggtgatcg accagcagcg
      721 cggcttctgg tggctcgatg ccgagccgaa agcggtgtcg gcgaaggccc accgtccggc
      781 ctacggatcg gacggcgact actggtcgaa accgaatcgc gagcaggtgt ttcgtgtggt
      841 ctacggattg atgcacgagg cccaccccga ccggttcccg atcttctatt gagccggcga
      901 gggtctgccg atgggtgagc gggtcctttc ccggttgcgc cccggggact accggctgcc
      961 ggacgagctg gtcggccggc tgctgacacc cgccctggtc atctacatcg atctcgtgcg
     1021 cgacaatctc cgccgcgtga tcgaccgagc cggcggcgag gtggatcgac tccggatcca
     1081 cgtcaagacg gcgaagatcc cggcggtgtt cgccgagctc gtcgcggccg gcatccgcaa
     1141 ctacaagtgc gcgacgctgc gcgaggcacg tgagctgctg caggtgctgc acgatcggca
     1201 ggtgacggcg agcgacctgc tggtcgccta tccgctgcga gagccggcgc tcggtcaggc
     1261 ggccgagttg gcgcgccgct tcccggccac gcgcctgtcg gtgctgtgcg aggacccgac
     1321 gaacctgcgg tcgattcccg aagagctgtc ggtgttcgtc gatgtcaacc cggggatgaa
     1381 ccgcaccggc gtgcccgcgg ccgacgaggc tcggatcacc gagctcgcac gcgcggcggc
     1441 cggccgtttc cgcgggctgc actactacga cggacatgta cacggcaagg acgcggcgga
     1501 gcggcgggcg atggcccacg acggctacgg gcgtctggtc gggctgaccg agcggctgtt
     1561 gcgacggtcg ctgccggtcg gtgaaatcgt caccagcggt acgccggcgt tcgttcacgc
     1621 gctgggattc gacccactgc ggtcgttcga ccacacggtg catcgggtct ctcccggtac
     1681 ggtcgtctac cacgacgcgc ggtacgcgct ggagctcgac gagctcgagc tgactccggc
     1741 cgcattggtc tggacccgcg tcgtcagccg gcctgccgac gagatcgcca cctgcgacgc
     1801 cggctcgaag tcgatcgccg ccgaggccgg cgatccgtgc gccttggtcg tcggccatcc
     1861 ggactaccgc gcgttgcgac cgagcgagga gcatctgccg ctcgccgttc cgccggcgga
     1921 cgcgccgcgt ctcggcgcga tgctgacact gatcccgcgt cacgtctgtc cgaccgtcaa
     1981 tctcgccgag caggcgctgc tcgtcgagcg tgggcgatcg ccgcgggtcg tctcggtcag
     2041 cgcgcgtgcg cacgacctga tcgccgggga gtgaccgggt gctgcgcggc gggtgccgca
     2101 tgatgctcct gggggtgtgc ctcctcgtgg cgccgcggcc ggaggccgcc gactgggaga
     2161 tcgtcccgtt cgcgggcttt cgctggggcg gcgaggtgac ggagatcgac accaatcgga
     2221 cggtcgattt cgacccgtcc gcggttttcg gcctgaccgt cggccgctac ctgtcgtcgg
     2281 agtcgcgcgt cgagggaacg tggagtcgcc aggcgagctc gctcgccgga gacggtctcg
     2341 acatcacgct cgactacctg cacttcgccg gtgtctacga gccgcacccc gcgcgcgcgt
     2401 cgacggctta cgtgctcgtc tcgggcggcg tgacccggtt cgacgcgagc ggggcggtcg
     2461 cgacgaccca tctgtcgtt
//
LOCUS       DASPGN010000309         3243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1225846, whole genome shotgun sequence.
ACCESSION   DASPGN010000309 DASPGN010000000
VERSION     DASPGN010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1225846"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="VD788_08980"
     CDS             <1..1547
                     /locus_tag="VD788_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXV76442.1"
                     /translation="HVEQADLQQWLQLEMPAAVVGAPTRVALTDDELAEIDRRDAEAP
                     GMTVVGRTKRLGRRVDFGEIGASLESGVERPFAGGFVRGTDDGGFVWATALTSDGASA
                     LRVGFAGVRLPSSTDLYVHNLVGQAFGPYSRRDLTPAGELWSNSVLGDTVIVQLRQWG
                     PATDAELGAISLSIEELGHIGPKFRPAVVAATREFCEQNASCVENTNCSGGPSWSAKA
                     DAEAATALMLWISGAFINTCTGGLVSDTDGSSQIPYVLTANHCVSKNADAANMEFYFQ
                     FEVACGGNCPNEWAGGGIQRLGSTLLATNRSGDYTLLQMSQSPPSGSSLMGWTTANVA
                     NADGTTLYRISHPATAPQAYSAQTVDTSAGTCRGVPRGDWIYSRDIYGATEGGSSGSP
                     VYNGAGQIVGQLTGACGFSPGDPCASEDNATIDGAFANYFDAIDQYLDPSPCSPSPED
                     CDDGADNDCDGATDCSDADCSGDPACGSSGCVNPGGAPAGASCSSNADCCSNKCKGPP
                     SNLTCR"
     gene            complement(1649..3076)
                     /locus_tag="VD788_08985"
     CDS             complement(1649..3076)
                     /locus_tag="VD788_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HXV76443.1"
                     /translation="MSPHSERPDIPATEFIGHPLALWQLFNIEMWERFAYYGMRAVLA
                     PYVALVFFGHLASGEANAEASLVYGGYTALVYATGIAGGYVADKILGFRRAILLGALI
                     MASGLFKPNISTMVGQLYAPGDVRRDSGFTIFYMGINLGALLAPLVTAAWIGAIFGLK
                     WGFFAAGIGMILSTLFLQLAKRSLGSVGLPPEGKEGWGRFFVVAGAALLSVPVIYFLL
                     SESAVLGYILIGIMFGLAIYLVVAGIRSGDKVQLHRYIVMLILFVANAFFWSLFEQAG
                     SSLNFLAKDFVDLDTGLFTMNFAVFQSANPMFILLLAPLFAMLWPKLDRYKMNPSIPR
                     KFGLGLIQVGFGFYVLVFAIGNMQDANGLIPWSMLLLCYFFHTTGELCLSPIGLSMVT
                     KLAERREVGLAMGGWFLSIAMAQYVAGLIAAVASGGEGGHAEAASSITQYASVYWQLF
                     WFGLIAGFVYLLVAPLLNKLMHGVR"
BASE COUNT          591 a         1088 c         1110 g          454 t
ORIGIN      
        1 accacgtcga gcaggccgac ttgcagcagt ggctccagct cgagatgccg gcggcggtcg
       61 tcggagcgcc gacgcgcgtc gcgttgaccg acgacgagct ggcggagatc gaccggcggg
      121 acgccgaagc gcccggcatg acggtcgtcg gccgcaccaa gcggctcggg cggcgagtcg
      181 acttcggcga gatcggcgcg agcctcgaga gcggagtcga gcggccgttc gccggcgggt
      241 tcgtccgcgg cacggacgat ggcggcttcg tctgggcgac ggcgctgacc tcggacggtg
      301 cgagcgcgct gcgcgtcgga ttcgccgggg tgcgcttgcc cagctcgacc gatctctacg
      361 tgcacaacct cgtcggccag gcgttcggac cctactcgcg ccgcgatctg acgccggcgg
      421 gcgagttgtg gtcgaacagt gtgctcggcg acacggtcat cgtccagttg aggcagtggg
      481 gaccggcgac cgatgccgag ctgggcgcga tcagcctgtc gatcgaggag ctggggcaca
      541 tcgggcccaa gttccgcccg gccgtcgtcg cggcgacgcg cgagttctgc gagcagaacg
      601 cgtcgtgcgt cgagaacacc aactgcagtg gcggcccgtc ctggagcgcc aaagcggacg
      661 ccgaggcggc gaccgcgctg atgctgtgga tttccggcgc gttcatcaac acctgtacgg
      721 gcggcctggt gagcgatacc gacgggtcct cgcagatccc gtacgtgttg accgccaacc
      781 actgcgtcag caagaacgcc gacgccgcga acatggagtt ctacttccag ttcgaggtcg
      841 cctgcggcgg aaactgcccg aacgagtggg ccggcggcgg gatccaacgg ctcggctcga
      901 cgcttctcgc gaccaatcgc agcggggact acacgctgct gcagatgagc cagtcgcctc
      961 cgtccggcag ctcgctgatg ggctggacga cggcgaacgt ggccaacgcc gatggcacga
     1021 cgctctaccg catcagccat ccggcgaccg cgccgcaggc ctactcggcc cagaccgtcg
     1081 acacgtcggc cgggacctgc cgcggcgtgc cgcgcggcga ctggatctac agccgagaca
     1141 tctacggcgc gaccgagggt gggagcagcg gctcgccggt ctacaacggc gcgggccaga
     1201 tcgtcggcca gctgaccggc gcctgcggtt tcagcccggg cgacccctgc gcgtcggaag
     1261 acaacgccac gatcgacggt gcgttcgcca actacttcga cgcgatcgac cagtatctcg
     1321 atccgtcacc gtgcagcccg agtcccgaag actgcgacga cggcgccgac aacgattgtg
     1381 acggcgcgac cgactgcagc gacgccgact gctcgggaga tccggcctgc ggctcgagcg
     1441 gatgcgtcaa tcccggcggg gcccccgccg gcgcgagctg ctcgagcaat gccgactgct
     1501 gctcgaacaa gtgcaaggga ccgccgagca acctgacctg ccgctgaaga tcgacgacga
     1561 ccccgacccg cgaggtcggg cacggggcgg aggggggccc ccctccgccc ttttttcgtc
     1621 gagcgcgccg ttgcgcgccg cgggagactc accgcacgcc gtgcatcagc ttgttgagca
     1681 gcggcgcgac cagcaggtag acgaaccccg cgatcagacc gaaccagaag agctgccagt
     1741 agaccgaggc gtactgggtg atgctcgaag cggcctcggc gtgtccgccc tcgccgccgc
     1801 tcgcgaccgc cgcgatcaac ccggcgacgt actgggccat cgcgatcgac aggaaccagc
     1861 cgcccatcgc cagtccgacc tcgcgtcgct cggccagctt ggtcaccatc gacaacccga
     1921 tcggcgacag gcacagctcg cccgtggtgt ggaagaagta gcacagcaac agcatcgacc
     1981 acgggatcaa cccgttggcg tcctgcatgt tgccgatcgc gaagacgagg acgtagaagc
     2041 cgaagccgac ctggatcaag cccaggccga acttgcgcgg gatcgacggg ttcatcttgt
     2101 agcggtcgag cttcggccac agcatcgcga acagcggcgc gagcagcagg atgaacatcg
     2161 ggttggcgga ctggaacacc gcgaagttca tcgtgaacag accggtgtcg aggtcgacga
     2221 agtccttggc caggaagttg agcgagctgc cggcctgctc gaacagcgac cagaagaagg
     2281 cgttggcgac gaacaggatc agcatcacga tgtaacggtg gagctggacc ttgtcgccgc
     2341 tgcggatgcc ggcgacgacc aagtagatcg ccaagccgaa catgatgccg atcaggatgt
     2401 agcccagcac cgcgctttcg ctcaacagga agtagatcac cgggacggac agcagcgccg
     2461 ccccggcgac gacgaagaac cggccccaac cttccttccc ctccggcggc agaccgacgc
     2521 tgcccaacga ccgcttggcc agctggagga acagcgtgct gaggatcatg ccgatgccgg
     2581 ccgcgaagaa gccccacttg agcccgaaga tcgccccgat ccacgcggcg gtcaccagcg
     2641 gcgcgagcag cgcgccgagg ttgatcccca tgtagaagat cgtgaagccg ctgtcgcgcc
     2701 ggacgtcgcc gggtgcgtag agctgcccga ccatcgtcga gatgttcggc ttgaacagcc
     2761 cggaggccat gatcagcgcg ccgagcagga tcgcgcggcg aaagccgagg atcttgtccg
     2821 cgacgtatcc gccggcgatg ccggtggcgt agaccagcgc ggtatagcca ccgtagacca
     2881 ggctcgcctc ggcgttggcc tcgcccgagg ccagatggcc gaaaaagacg agcgcgacgt
     2941 acggcgccag gacggcgcgc atgccgtagt acgcgaagcg ctcccacatt tcgatgttga
     3001 acagctgcca cagggccaac gggtgcccga tgaactcggt cgcggggatg tcgggacgct
     3061 cggaatgtgg gctcacgggt tcctccacgc gggatggtgc gggatacggc gccactcgtc
     3121 gaatccggcc gattctacag aattcgaacg gtcggtccaa ttccgggctg cagaccggcc
     3181 gcccgtgcgt actttgacgg atacgacact cccgcttggc gcgtcgcgtc cggaggtccc
     3241 ttg
//
LOCUS       DASPGN010000310         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_524334, whole genome shotgun sequence.
ACCESSION   DASPGN010000310 DASPGN010000000
VERSION     DASPGN010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524334"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VD788_08990"
     CDS             complement(<1..767)
                     /locus_tag="VD788_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HXV76444.1"
                     /translation="MSSYVSSLMEDVKAKNPAEPEFHQAVAEVAESLVTVLDKHPEYR
                     SAKILERIIEPERVIMFRVPWTDDQGHVRINRGFRIEMNSAIGPYKGGLRFHPTVYLG
                     LLKFLAFEQVFKNALTTLPMGGGKGGANFDPKGRSDGEVMRFCQSFMIELQRHIGADT
                     DVPAGDIGVGGREIGFLFGQYKRIRNEFTGVLTGKGLNWGGSLIRPEATGYGCVYFAQ
                     EMLATRNQTLEGKVCLVSGSGNVAQYTIEKLLDLGAKP"
     gene            complement(945..>2623)
                     /locus_tag="VD788_08995"
     CDS             complement(945..>2623)
                     /locus_tag="VD788_08995"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF003820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HXV76445.1"
                     /translation="PHELVTYGGNGTVFQNWAQYLLTMRYLSRMTDEQTLVLYSGHPL
                     GLFPSQLDAPRLVVSCGLVVPNHSRPEDYERLAALGVTSYGQMTAGSFMYIGPQGIVH
                     GTTITLLNAGRKYLGIEGDRGLGGRVYVTSGLGGMSGAQAKAAVIAGAVGVIAEIDPQ
                     ALSKRRQQGWLQEVEADLDALLSRVERARRDEQALSIGYLGNVVDLWERLAAAGCAVD
                     LGSDQTSLHNPFGGGYYPAGLSLAQSKSMLRADPTGFAERVRESLRRQVDAINVVAER
                     GMHFWDYGNAFLLEASRAGAACVGADGRFRYPSYVEDIMGPMCFDYGFGPFRWVCTSG
                     EQSDLEQSDAIAAAELESMARDAPREIRQQRLDNLRWIREAGRQRLVVGSRARILYAD
                     EQGRKRIALAFNRAVAAGQITAPVVLGRDHHDVSGTDSPYRETADVRDVSNPCADMAV
                     HNVIGDAFRGATWVSLHNGGGVGWGEAINGGFGLLVDGTAECEHRIRRMIAWDVTNGL
                     VRRAWARNPGARWSVDRLMRDEPLLDATLPHEVDPALVDKTVRDTGKSGS"
BASE COUNT          403 a          915 c          878 g          427 t
ORIGIN      
        1 ggcttcgcgc ccaggtcgag gagcttctcg atcgtgtact gggccacgtt gcccgagccc
       61 gagaccaggc agaccttgcc ctccagcgtc tggttgcgcg tggcgagcat ctcctgggcg
      121 aagtacacgc aaccgtagcc ggtggcctcg ggacggatca gcgagccgcc ccagttcagg
      181 cccttgccgg tcagcacgcc ggtgaactcg ttgcggatcc gcttgtactg gccgaacagg
      241 aagccgatct cccggccgcc cacgccgatg tcgccggcag gcacgtcggt gtcggcgccg
      301 atgtgacgct ggagctcgat catgaaggac tggcagaacc gcatgacctc gccgtcgctg
      361 cggcccttgg ggtcgaagtt cgcgccgccc ttgcccccgc ccatcggcag cgtcgtgagc
      421 gcgttcttga agacctgctc gaacgccagg aacttcagca ggcccaggta cacggttgga
      481 tggaagcgca ggccgccctt gtaggggccg atcgcgctgt tcatctcgat gcggaatcca
      541 cggttgatgc ggacgtgacc ctggtcatcg gtccacggca cgcggaacat gatcacgcgc
      601 tcgggctcga tgatccgctc gaggatcttg gcgctgcggt actcggggtg cttgtcgagt
      661 acggtgacca gcgactcggc gacctctgcc acggcttggt ggaactccgg ctccgccggg
      721 ttcttggcct ttacgtcttc catcaggctc gacacgtacg aactcatcgg ttttcctccc
      781 ggagaagaac cggaccgacg tgaccggccc ctccgcacaa atcggtcgta ggcgggcggc
      841 tgcgcgcatg ctcgggggag acgccgggcc ggctggcgcg gggacggtcc ggagacggat
      901 gctgcaccag accaccccgg gtgtcaacgg cggttgccac ccttttagga cccgcttttc
      961 cccgtgtctc tcaccgtctt gtccaccagc gccgggtcga cctcgtgcgg gagcgtggcg
     1021 tcgagcaacg gctcgtcgcg catcagccgg tcgaccgacc atcttgcacc ggggttgcgc
     1081 gcccaggcgc ggcgcaccag tccgttggtc acgtcccagg cgatcatccg ccggatacgg
     1141 tgctcgcact cggccgtacc gtcgaccagc aggccgaaac cgccgttgat cgcctcgccc
     1201 cagccgactc cgccgccgtt gtgcaggctg acccacgtcg cgccgcggaa ggcgtcgccg
     1261 atgacgttgt gcacggccat gtcggcgcac gggttgctga cgtcgcggac gtcggcggtt
     1321 tcgcggtacg gcgagtcggt gccggacacg tcgtgatgat cacggccgag cacgaccggt
     1381 gcggtgatct gacccgcggc gaccgcgcga ttgaaggcga gcgcgatgcg cttgcggccc
     1441 tgctcgtcgg cgtacaggat gcgcgcgcgg gagccgacga ccagccgctg ccggccggcc
     1501 tcgcggatcc aacgcaggtt gtcgagccgt tgctggcgaa tctcgcgcgg tgcgtcgcgc
     1561 gccatcgatt ccaactcggc ggcggcgatc gcatcgctct gctcgagatc gctctgttcg
     1621 ccgctggtac acacccagcg gaacggcccg aagccgtagt cgaagcacat cggccccatg
     1681 atgtcttcga cgtaagacgg gtagcggaag cgaccgtcgg ccccgacaca cgccgcaccc
     1741 gcgcgcgatg cctcgagcag gaacgcgttg ccgtagtccc agaagtgcat gccgcgttcg
     1801 gccacgacgt tgatcgcgtc gacctgccgg cggagcgact cgcgcacgcg ctcggcgaag
     1861 ccagtcggat cggcgcggag catcgacttc gattgcgcga gactcagccc cgccgggtag
     1921 tagcccccgc cgaacgggtt gtgcagcgac gtctgatccg agccgagatc gacggcgcag
     1981 cccgccgcgg cgagccgttc ccacaggtcg acgacgttgc ccaggtagcc gatcgacagc
     2041 gcctgctcgt cgcggcgcgc tcgctcgacg cgcgacagca gcgcatcgag gtcggcttcg
     2101 acctcctgca accaaccctg ctgccggcgc ttgctcaacg cctgcgggtc gatctcggcg
     2161 attacaccga ccgccccggc gatcaccgcg gccttggcct gcgcaccgct catgccgccg
     2221 agccccgacg tgacgtagac ccggccgccc agaccgcggt cgccctcgat gccgaggtac
     2281 ttccgtccgg cgttgagcag cgtgatcgtc gtcccgtgga cgatgccctg cggaccgatg
     2341 tacatgaagc tgccggccgt catctgaccg tacgacgtca cgccgagcgc ggccagacgt
     2401 tcgtagtcct ccgggcggct gtggttggga acgaccaagc cgcagctgac gaccagccgc
     2461 ggggcatcga gctgcgacgg gaacagacca agcgggtgtc cggagtagag caccagcgtc
     2521 tgctcgtcgg tcatccgcga caggtagcgc atcgtcagca ggtactgcgc ccagttttgg
     2581 aagaccgtgc cgttgccgcc ataggtcacc agctcgtgcg ggt
//
LOCUS       DASPGN010000311         7115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_641385, whole genome shotgun sequence.
ACCESSION   DASPGN010000311 DASPGN010000000
VERSION     DASPGN010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641385"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VD788_09000"
     CDS             complement(<1..327)
                     /locus_tag="VD788_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76446.1"
                     /translation="MPAIDEADGRRVRRWLRLAWKALVWLVVVAALLLVVGLAIVLVP
                     VARDPSPRFAERKGELVAFRETRRWDDGRDMLSEMTLVSSSGLEVELTARIPREATGP
                     RPGLVLL"
     gene            complement(357..1169)
                     /locus_tag="VD788_09005"
     CDS             complement(357..1169)
                     /locus_tag="VD788_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76447.1"
                     /translation="MVVVLLALPASAANINGFLRDEGEFDVAISYNAESYDEFWAGNE
                     ITSAPPLGEVSTNTASLWLAYGVTESISVWANIPYVDVEGDGSGNLSDSGLQDLIAMG
                     EFRLAGKPHGEGWGVLIGGIGFSTPLSDYEADAPVSLGDGTTDVLARLVYQFQRNRFY
                     ASGQVGYDLRGDDAPDGYPFYVEAGYTAGPLTISGGYAKLVADGGTDIGDPGFTFPSN
                     QEEFDRTGLKLYGRVTPTFGLVGTWFTTLDGRNTGDSSGGSLGAVFRFDFAE"
     gene            1678..2994
                     /locus_tag="VD788_09010"
     CDS             1678..2994
                     /locus_tag="VD788_09010"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HXV76448.1"
                     /translation="MKRLLRTTCLALTIVTCTGVAGADVVTDWNNHALDAIRATNTNP
                     PRATRALAMVHTAMFDAINGIERVYAPYHVSRPAPPGASPQAAAAWAAYTVLVGLYPD
                     REWEFGEALESSLAGERFGPAQWKGRAFGRYVGRAILTLRADDGADAFVAYAPSGEFG
                     YWQPTPPAFGPALLPQWPAVTPFAMTHGSQFRVPAPPPFDSAEYAAAFEEVRQLGDKS
                     SGTRDPDQTEIAYFWEDGPGTATPPGHWQVIAQQLAEMFDHDLLDNARLFALLSIVQA
                     DGAIVSWDNKYHHDYVRPYTAITAEADDDGNPETASDPGWSTLIPSPPFPSYTSGHST
                     FSGGSARLLGHYFGTDAVSFCAPSPDPQRWPEQLEGVVRCWNSLAEAAEEAGQSRIYG
                     GIHWQYDNQQGLDSGRALADYVWAEFLTPLPQAAEAPRGRGGARWR"
     gene            3084..4679
                     /locus_tag="VD788_09015"
     CDS             3084..4679
                     /locus_tag="VD788_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV76449.1"
                     /translation="MAPPSKLAAEVRLGWWAAPLVVGVLLVNLAGVWEIAAALRGLRE
                     HGERMLRAETAALARTVESRLAATTVDLAFLVGSPALAADAVARDDRWRDEAGTALLL
                     FLRGHPEVRDLVVRDDAGSSLIEAGRQRGVPGFWTPGPGSPSLGAAEPAMRREFEVSG
                     GGAGGPGRLEASLDPRQLVDGSSAQNTQRAECALVDAGGSRLAGAERAANSAEAAESA
                     VGDADWGAPGPWKLRCVAVGATPGAELEPLVARHRMTILLNLVVMALAVGLGTFALQQ
                     QRRRRELEQRARHEAEVRELERQLFHAERLGTVGRLAAGMAHEINNPLEGIANYLRLA
                     AEAVERGDGESARRHLDGVRQGTERAAAILRRVLDHADPSTALVEEVDVGDVVSRSIE
                     FVRARPEFASIRFAVELPTPPARTRGSSVLLGQVFLNLVLNACEAQPAGGEVAVRCAV
                     HARGVDVEVADRGPGVPPARRGRIFEPFESTKRSAGLGLSICHSIVERHGGELSVHER
                     DGGGAVFRVRLVAAEVERTDGAA"
     gene            4666..6063
                     /locus_tag="VD788_09020"
     CDS             4666..6063
                     /locus_tag="VD788_09020"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV76450.1"
                     /translation="MGPRKPARGRILIVEDEEYVRRSLAELLESRGYDVTAAGSAEEA
                     FGRLARAPVDVVLSDLRLAGTDGLTLVRRLRDGYAELPVVILTGHGTIASAVDCIRSG
                     ATDYILKPADPDVLEVSLDRALATRAMKRELAHLRNREEALGESDRPVGESAVWHALM
                     RQVRAAAPTDSTVLLRGESGTGKELLARLLHRLGPRAGRAFVRVNCSATPVEMWESEF
                     FGHRRGSFTGATSDRDGRFLAAHRGTLFMDEIGTMPLAAQAKILRVLEDGEFQRLGDE
                     QPTRVDVRIIAATNSDLEAELEAGRFRQDLFYRLNVLPIRVPPLRERLDDLPLLVERL
                     VAEIAARLNRPVPRIAAHALDELAAYSWPGNVRELRNVLERALILNPDELVTALELGP
                     LEAGGATEVHDRSDLNLRRRLAVAERNLVVDALRRAGGVRKEAARLLGIDQRNLPYYL
                     RKHEIEPDTQSGHDD"
     gene            complement(6125..>7115)
                     /locus_tag="VD788_09025"
     CDS             complement(6125..>7115)
                     /locus_tag="VD788_09025"
                     /inference="COORDINATES: protein motif:HMM:NF020920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HXV76451.1"
                     /translation="LTGRHGFFSCYEAFIHIVDSMFNQHAKWLKACRHIPWRRSIASL
                     NYLLSSHVWRQDHNGFSHQDPGFIDLVVNKKAEVVRVYLPPDANCLLSVTDHCLRSRN
                     YINVVVAGKQPAPQWLAMDDAIKHCSAGIGIWSWASNDQSGEPDVVMACCGDVPTLET
                     LAAVDLLRRHLPEIKVRVINVVNLMKLQPEREHPHGLSDAEFDVLFTDDKPIIFAFHG
                     YPWLIHRLTYRRTNHSNLHVRGYVEEGTTTTPFDMVVLNRMDRFSLVADVIDRVPKLG
                     PRAAYAKQAIRDRLIEHRQYIREHGEDMPAIRNWTWGGGDTDRGPASTEADNF"
BASE COUNT         1071 a         2473 c         2493 g         1078 t
ORIGIN      
        1 gagcagcacg agccccgggc gcgggccggt cgcctcgcgc gggatccgcg cggtgagctc
       61 cacctcgagc ccgctcgacg agacgagcgt catctcggag agcatgtcgc gaccgtcgtc
      121 ccatcggcgc gtctcacgga acgcgaccag ctcacccttc cgctcggcga agcgcggtga
      181 cgggtcgcgg gcgaccggaa cgagcacgat cgcgaggccg acgacgagca ggagcgccgc
      241 gacgacgacg agccacacga gcgccttcca cgccaggcgg agccagcgac gaacccgtcg
      301 tccgtcggct tcgtcgatcg cgggcaacgg tctcctccgc gcgggagtgc gggtgactac
      361 tccgcgaagt cgaagcggaa gacggcgccg agtgaaccgc cgctcgagtc gccggtgttc
      421 ctgccgtcga gcgtggtgaa ccaggtcccg accaggccga acgtcggtgt gacgcgcccg
      481 tagagcttga gcccggtgcg gtcgaactcc tcctggttgc tcggaaaggt gaagccgggg
      541 tcgccgatgt cggtgccgcc gtccgcgacc agcttcgcgt agccgccgct gatcgtcagc
      601 gggccggcgg tgtaccccgc ttcgacgtag aacgggtagc cgtcgggcgc gtcgtcgccg
      661 cgcagatcgt agccgacctg cccggaggcg tagaagcgat tgcgctggaa ctggtagacg
      721 aggcgcgcca gcacgtcggt cgtcccgtca ccgagcgaga ccggggcatc ggcctcgtag
      781 tcggacagcg gtgtcgagaa cccgatcccg ccgatcagca cgccccaacc ctctccgtgc
      841 ggcttgcccg ccaggcggaa ctcgcccatc gcgatcaggt cctggagacc ggaatcggac
      901 aggttgcccg agccgtcgcc ttcgacgtcg acgtagggaa tgttggccca cacgctgatc
      961 gactccgtga cgccgtaggc cagccagagc gaggcggtgt tcgtgctcac ttcaccgagc
     1021 ggcggtgccg acgtgatctc gtttcccgcc cagaactcgt cgtagctctc cgcgttgtag
     1081 ctgatcgcca cgtcgaactc gccctcgtcc ctcaagaagc cgttgatgtt cgccgcactc
     1141 gccggcagcg ccagcaagac gaccaccacg aaaccgatcc cacggagaag attctcgcca
     1201 agcacgacag cccccttgtc tcgttcgtcc agccagtcga atcgtccgcc gggccacgcg
     1261 ggccaggtcg ccatgcaccc tacgcccaga ccgcgtggcc cgcaagatct aagaattacg
     1321 agttcggagc gcgaaaaatc ataagttata tggctttgca gccggccgat ttccggctgc
     1381 gtggatccac gtccgccgtc gttcatagga tcgacgacgc gtccgatcgc gtgcgccgaa
     1441 cgactccacg aaccgcggcc gcgccgcgac gcacataact ccgacgctcc gccatcttgt
     1501 ccgatcatag gcgctatgtc tcggcgcgat tcgcgaaccg ggagtcgacc aggacccgaa
     1561 acgggtaact catttctcca tcaagtactt gcgcgttcca cgcgccagag ttgcggcagt
     1621 tggcaagacc cttgcctagg gcggtcggca cacgatgctg caacgaggag aaccgcgatg
     1681 aaacggctgc ttcgaacgac ctgtctcgcg ctgacgatcg tcacctgcac cggcgtggcc
     1741 ggcgccgacg tcgtcaccga ctggaacaac cacgcgctcg acgcgatccg cgcgaccaac
     1801 accaacccgc cgcgggcgac gcgcgcgctg gcgatggtcc acaccgcgat gttcgacgcg
     1861 atcaacggga tcgagcgcgt ctacgcgccc taccacgtct cgcggccggc tcctcccggc
     1921 gcatcgcctc aggccgcggc ggcctgggcg gcctacaccg ttctcgtcgg gctctacccc
     1981 gaccgcgaat gggagttcgg cgaagcgctc gagagctctc tcgccggcga gcggttcggc
     2041 cccgcgcaat ggaaggggcg agccttcggt cgttacgtcg gacgggcgat cctgaccttg
     2101 cgcgccgacg acggagcgga cgcgttcgtc gcctacgcgc cgtcggggga gttcggttac
     2161 tggcagccca cgccgccggc cttcgggccc gcgctgctgc cgcagtggcc cgccgtcacg
     2221 ccgttcgcga tgacccacgg ctcgcagttc cgggtaccgg caccgccccc gttcgacagc
     2281 gccgagtacg cggccgcgtt cgaggaggtc cgccagctcg gtgacaagag cagcgggacg
     2341 cgcgacccgg atcaaaccga gatcgcctac ttctgggaag acggtcccgg caccgccacg
     2401 ccacccggac attggcaggt gatcgcgcag cagctcgccg agatgttcga tcacgacctg
     2461 ttggacaacg cccgcctgtt cgcgctgttg agcatcgtcc aggccgacgg ggcgatcgtc
     2521 tcgtgggaca acaagtacca tcacgactac gtccggccct acaccgcgat cacggccgaa
     2581 gccgacgacg acggcaaccc ggaaaccgcg tccgatcccg gttggtcgac gctgatcccg
     2641 tcgccgccgt ttccgtccta cacgtcgggg cacagcacct tcagcggagg ttcggcgcgg
     2701 ctgctcggtc attacttcgg cacggacgcg gtgtcgttct gcgcgccgtc acccgatccg
     2761 cagcgctggc ccgaacaact cgagggcgtc gtccgctgtt ggaacagcct ggccgaggcc
     2821 gccgaggagg ccggccagag ccggatctac ggcggcatcc actggcagta cgacaaccag
     2881 caggggctcg actccggccg ggctctcgcc gactacgtct gggccgagtt cctcacgccg
     2941 ctcccgcaag ccgccgaggc cccgcgcggg cgcggtggcg ctcgctggcg ctgagcttcc
     3001 gcgacgttac accgagcgag aagcacgcgt cggtgcagta tgatgcccgc tcgttcgagc
     3061 cgaacgaggc gaggtggatt tcggtggcac cgccctcgaa gctggcggcc gaggtgcggc
     3121 tcggctggtg ggccgcgccc ctcgtcgtcg gtgtcctgct ggtcaacctc gccggggtgt
     3181 gggagatcgc ggccgcgttg cgtggcttgc gcgagcacgg cgagcggatg ctgcgggccg
     3241 aaaccgccgc gctggcacgc accgtcgaaa gccggctggc agcaaccacc gtcgatctgg
     3301 cgtttctcgt cgggtcgccg gcgctggccg ccgacgcggt cgcgcgggac gatcgctggc
     3361 gggacgaggc cggcaccgcg ctgctgttgt tcctgcgcgg tcatccggaa gtgcgcgacc
     3421 tcgtcgtgcg cgacgacgcc gggagctcgc tgatcgaggc cggtcggcag cgcggcgttc
     3481 ccgggttctg gacgcccggt ccgggcagcc cgtcgctcgg cgcggcggag ccggcgatgc
     3541 gacgcgagtt cgaggtgtcg ggtggaggcg cgggcggtcc cggccgactc gaggcgtcgc
     3601 tcgatccgcg gcagctcgtc gacggctcgt ccgcgcagaa cacgcagcgg gcggaatgcg
     3661 ccctcgtcga tgcgggcgga tcccggttgg ccggggccga acgggccgcg aactcggccg
     3721 aggcggccga gtccgccgtc ggcgacgccg actggggtgc gccggggccg tggaagctcc
     3781 gctgcgtcgc ggtcggtgcg acacccggcg ccgagctcga accgctcgtc gctcgccatc
     3841 ggatgacgat cctgctcaac ttggtcgtga tggcgctcgc cgtcggcctc ggcaccttcg
     3901 cgctacagca gcagcgccgc cggcgcgagc tcgaacagcg cgcccgtcac gaggccgagg
     3961 tgcgcgagct cgagcgccag ctgttccacg ccgagcggct cggcacggtc gggcggttgg
     4021 cggccggcat ggcccacgag atcaacaacc cgctcgaggg catcgccaac tacctgcggc
     4081 tcgccgccga ggcggtcgag cgtggcgacg gggagagtgc gcggcggcac ttggacggcg
     4141 tccgacaggg gaccgaacgc gctgccgcga tcctgcgccg cgtgctcgat cacgccgacc
     4201 cgtcgacggc gttggtcgag gaggtcgacg tcggcgacgt cgtctcgcga tcgatcgagt
     4261 tcgtccgtgc gcgccccgag ttcgcctcga tccgcttcgc cgtcgagctg ccgacgccgc
     4321 ccgcccgtac ccgaggcagc agcgtgctgc tcggccaggt ctttctcaat ctcgtgctca
     4381 acgcgtgcga ggcgcagccg gccggcggag aggtggcggt ccgctgcgcg gtccacgcgc
     4441 gcggggtcga cgtcgaagtg gccgaccgcg ggccgggcgt gccgcccgcc cgccgcggac
     4501 gtatcttcga accgttcgaa tcgaccaaac gatcggccgg gctcggcctg tcgatctgcc
     4561 actcgatcgt cgaacggcac ggtggcgagc tgtcggtgca cgagcgcgac ggcgggggcg
     4621 cggtcttccg agtccggctg gtcgcggcgg aggtggaaag gacggatggg gccgcgtaaa
     4681 ccggctcgag gacgcatcct catcgtcgag gacgaggagt acgtccgccg atcgctggcc
     4741 gagttgctcg agtcgcgcgg ctacgacgtc accgccgcgg gttcggcgga ggaggcgttc
     4801 gggcgactcg cacgcgcgcc ggtcgacgtc gtgttgtcgg atctgcggct tgcgggcacc
     4861 gacggcctga cgctcgtgcg gcgtctgcgg gatggctacg ccgagctgcc ggtcgtgatc
     4921 ctcaccggcc acgggacgat cgcgtcggcc gtcgactgta tccgttccgg cgccaccgac
     4981 tacattctca agcccgccga tcccgacgtg ctggaggtca gcctggaccg tgcgttggcc
     5041 acgcgggcga tgaagcgcga gctcgcccac ctgcggaatc gcgaggaggc gctcggcgaa
     5101 tcggatcgcc cggtcggcga gagcgcggtg tggcacgcgc tgatgcgcca ggtgcgcgcc
     5161 gcggcgccga ccgactcgac cgtgctcttg cgcggcgagt ccggcacggg caaggagctg
     5221 ctggcgcgac tgctgcaccg gctcggcccg cgtgccggcc gggccttcgt ccgcgtcaac
     5281 tgctcggcca ctccggtcga gatgtgggag agcgagttct tcggccatcg ccgcggctcc
     5341 tttaccggcg cgaccagcga ccgtgacggg cggttcctcg ccgctcatcg cggcacgctg
     5401 ttcatggacg agatcggcac gatgccgctg gccgcgcagg cgaagatcct gcgcgtgctc
     5461 gaggacggcg agttccagcg cctgggcgac gagcaaccga cacgggtcga cgtgcgcatc
     5521 atcgcggcga cgaacagcga tctcgaggcc gagctcgagg cgggacgctt ccgccaggat
     5581 ctgttctacc ggctcaacgt gctgccgatc cgcgtgcccc cgttgcgcga gcggctcgac
     5641 gacctgccgc tgttggtcga gcggctggtc gcggagatcg ccgcgcggct gaaccggccc
     5701 gtcccgcgca tcgcggccca cgcgctcgac gagctggccg cctactcctg gccgggcaac
     5761 gtgcgcgagc tacgcaacgt cctcgagcgg gcgctgatcc tcaacccgga cgagctggtg
     5821 actgcgctcg agctcgggcc gctcgaggcg gggggggcga cggaggtcca cgaccgctcc
     5881 gatctcaacc tgcgccgccg gctcgccgtc gccgagcgca acttggtggt cgacgcgctc
     5941 cgtcgcgccg gcggcgtgcg caaggaagcg gcccgtctgc tcggcatcga ccagcgcaac
     6001 ctgccgtact acttacgcaa gcacgagatc gagcccgaca cgcagagcgg ccacgacgac
     6061 tagcctagcg gcgcgaatca cagcgggtcg tgggatggcg cgcagcgaag gctcgacgag
     6121 ccgctcagaa gttgtcggcc tcggtcgatg ccgggccccg gtccgtatcg ccgccgcccc
     6181 aggtccagtt gcggatcgcg ggcatgtcct cgccgtgctc gcggatgtac tggcgatgtt
     6241 cgatcaggcg gtcgcggatc gcctgtttgg cataggccgc gcgcgggccc agcttcggca
     6301 cgcggtcgat cacgtccgcc accaggctga agcggtccat ccggttcagc accaccatgt
     6361 cgaacggcgt cgtcgtcgtg ccttcctcga cgtagccgcg cacgtgcagg ttggagtggt
     6421 tggtccggcg gtaggtcagg cggtggatca gccagggata gccgtggaag gcgaagatga
     6481 tcggcttgtc gtcggtgaac agcacgtcga actcggcatc ggacaacccg tggggatgct
     6541 cgcgctcggg ctggagcttc atcaggttga cgacgttgat cacccgtacc ttgatctcgg
     6601 gcaggtggcg gcgcagcaag tcgacggcgg ccagcgtctc gagcgtcggc acgtcgccgc
     6661 aacaggccat caccacgtcc ggctcgccgc tctggtcgtt gctggcccag ctccagatgc
     6721 cgattcccgc cgagcaatgc ttgatcgcgt catccatcgc gagccactgc ggcgcgggct
     6781 gcttgccggc gacgacgacg ttgatgtagt tacggctgcg caggcagtgg tcggtcacgc
     6841 tcagcaggca gttggcgtcg ggtggcagat agacccgcac cacctcggcc ttcttgttga
     6901 ccaccaggtc gatgaagccg ggatcctggt ggctgaaacc gttgtggtcc tggcgccaga
     6961 cgtgactcga cagcaggtag ttcaacgacg cgatcgatcg ccgccacgga atgtggcggc
     7021 aggccttgag ccacttggca tgctggttga acatcgagtc gacgatgtgg atgaacgcct
     7081 cgtaacagga gaagaagccg tggcggccgg tgagc
//
LOCUS       DASPGN010000312        10122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_876255, whole genome shotgun sequence.
ACCESSION   DASPGN010000312 DASPGN010000000
VERSION     DASPGN010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876255"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..3297
                     /locus_tag="VD788_09030"
                     /pseudo
     CDS             1..3297
                     /locus_tag="VD788_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            3325..3942
                     /locus_tag="VD788_09035"
                     /pseudo
     CDS             3325..3942
                     /locus_tag="VD788_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            3996..5507
                     /locus_tag="VD788_09040"
     CDS             3996..5507
                     /locus_tag="VD788_09040"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HXV76452.1"
                     /translation="MSGEPSPDRVAPEAKTGLDPWVEGELPPPPEPTGLAWLGVVGPG
                     VIVLGASIGSGEFLLGPAAFVKYGLSLLWVTGLAAFLQTVFNTELMRYTLATGEPIFS
                     GFMRTRPGARFWGFVYSLLYVLQVGWPGWAGAAAGAVFFLFAGRVAATGDEHAVYLIG
                     VGTFAACVAILAVGRRIERTLEILNWILVAMILGGFVGLAVALVAPQTWLAAIAGFIG
                     FAPATGRFQLIPADADFFLIGAFAAYSGAGGMINLTLSNWARDKGYGMAAGAGYIPAA
                     TAGRHVHLTHTGFRFEPDAAALERWRGWWRVVRADQWGVYFIGAIVGMALPGMIYVTF
                     LPAGSDIRGLGIAAALADALRERAAPWIGIFVALLGVWVLFKTQLDILDGVTRALTDI
                     LWTGSRRLRRNPNADVRAVYYGVLLALSLWGVVALRLTQPIVLLQIGANMAGIVFIVT
                     SIHLLRVNTTLLPPELRPPTWRRLVLIGVAVFYGSFVSLWLASLVGRSGTALG"
     gene            complement(5540..5950)
                     /locus_tag="VD788_09045"
     CDS             complement(5540..5950)
                     /locus_tag="VD788_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76453.1"
                     /translation="MRATTSLALLCMTVFAATGCASLSIEARGGSSTVPEPPPKAGPG
                     HSTPSRLGIPPGHFPPAGQCRVWHPGRPPGHQPAPGSCSVVEQDVGPGDWLVYRPTSE
                     KKVVRVSFYDSRSPRVRIAVRVYDYRTGEFLREM"
     gene            6222..6848
                     /locus_tag="VD788_09050"
     CDS             6222..6848
                     /locus_tag="VD788_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76454.1"
                     /translation="MMHERQSKETWLVLALALPLIAVGCAGEPAAEIDAASAALESAR
                     LAEAEVYAPEAMQTAEAAHAELQTELAAQKERFALGRSYDKTRELAADAQRASEEAVG
                     AAHAGKLMAREEAMNMLAELRTSLDEVETMLDNAPQGKGSAADLAALRGDLGAVDVAL
                     TDIEQAISAQRFIDAVQKAQAAMASTAEIRSEIERAQEAQRAAKSRRG"
     gene            6899..8050
                     /locus_tag="VD788_09055"
     CDS             6899..8050
                     /locus_tag="VD788_09055"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXV76455.1"
                     /translation="MIVLGSGVSVLLAVSVHHAFTTRAAPPALLALEARRALDRADDL
                     GAREWALRPLVEAASALDAGRAEERRQRSRWLPFRDFGEARASYRRAIDAAKRAEEAA
                     AEANAVMIGRVETRLARVEEIVGQVVGLTRGMRLEQAESRSLRVARLRLSEARVQAES
                     GRYRSADRLAGEALAHTRRGVDCLRDRAARYVDRNHVRRWRAWEAETIVWSRRHRAAA
                     IVVNKEHNRLTLYVAGKPERDFHADLGRSHFDAKLRAADGATPEGRYRITRKKGKGRS
                     TYYKALELDYPNEDDRRRHAEAVSNGRVPRRVGAGDSIEIHGAGGRDDDWTQGCVALS
                     NDDIDYVFDAVRVGTPVTIIGGDGGGGRFSSLAARLADAREELDGFVRN"
     gene            8091..8762
                     /locus_tag="VD788_09060"
     CDS             8091..8762
                     /locus_tag="VD788_09060"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXV76456.1"
                     /translation="MTIRLVRRGKALSVAAVALAVAVLGVSTSGYRYRVEALPLTRTE
                     QALPTETAALQAFVADQRREQARLDRQLRRASPRSTYIVIDRTHNRLQLRRNGETMRE
                     ATCSAGSGMVLRDEARDREWVFDTPQGRFEVLRKIEDPVWKKPDWAFIEQGQPLPADP
                     GERIEYGVLGEYALYFGDGYMLHGTLYERLLGRSVTHGCVRLGREDLRAVYHNSAVGT
                     PIFIY"
     gene            8770..9447
                     /locus_tag="VD788_09065"
     CDS             8770..9447
                     /locus_tag="VD788_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76457.1"
                     /translation="MRMIRRNPIATTAVLLPLLGVGFLGFAAGVPDDDDGARTLRERA
                     LRLVHGNTCRRAEAELVEGERFYLVLDIDRRSLSLKLGGVMLHELPIESIEVGRPYRF
                     FFEHDGPKPIRARLHRQSSLSPPRLPLRLPVEIPEEADVETQTIMLPPEPETAIAVPR
                     RFFIRYREGVVLEFRTGRRDLGQRINDRVAALFRGPELRLRLVLERRAAEIFYRSVPR
                     ETDLLVL"
     gene            9569..>10122
                     /locus_tag="VD788_09070"
     CDS             9569..>10122
                     /locus_tag="VD788_09070"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXV76458.1"
                     /translation="MRKIGLFLSCLLISGVAVADPATFVEIALIDGGENGRNGSGDQF
                     GFEATGDYGFNEHWYVGGALGQFERDSSGGDIENTYFNVHGGYVVPVADPTGVNLEAG
                     LWLGDQDNPGDEDTDPRAFEIKAGVNHSLSDKFGIFGTLAWVHGDLDTPQDSDLRNYV
                     WSLGGAYSISERVSINLKIVNGVNG"
BASE COUNT         1588 a         3517 c         3552 g         1465 t
ORIGIN      
        1 tgcgacgcct gcccgctcga cgccgccaac gatgccgacc aggacggcgt ctgcggcgac
       61 ctcgacaact gccccaccgc gccgaacgcg gcgcaagccg atgcggacgc cgacggcgcg
      121 ggcgacgcct gcgacgcctg cccgctcgat cccggagacg acgccgatgc cgacggcgtc
      181 tgtggcgatc tcgacaactg cccggccgtg ccgaacgcag cgcaaaccga cgcggacgcc
      241 gacggcgcgg gtgacccctg cgacgcctgc ccgctcgacg ccgctgacga cgcggaccag
      301 gacggcgtct gcgctgacgc cgacaactgc ccgaccgtgt cgaacgcggg acagagcgac
      361 gcggacgccg acggcgcagg cgacgcctgc gacgcctgcc cgctcgacgc cgacaacgac
      421 gccgacggcg acggcgcctg cggcgacgcg gacaattgtc ccgtcgacgc caaccccgct
      481 cagaccgacg cggacgccga cggcgcgggt gacccctgcg acgcctgccc gctcgacgcc
      541 gacaacgatg ccgaccagga cggagtctgc ggcgacgccg acaactgccc ggccgtgccg
      601 aacgcggcac agggcgacgc ggacggcgac gccacgggcg acgcctgcga cgcctgcccg
      661 ctcgacgccg cgaacgatgc cgaccaggac ggagtctgcg gcgacagcga caattgcccc
      721 gtcgacgcca acgcggctca gaccgacggc gacgccgacg gcgcgggcga cgcctgcgac
      781 gcctgcccgc tcgacgccga caacgacgcg gaccaggacg gggtctgcgg cgacgccgac
      841 aactgtccgg cgatcgccaa ccccgttcag tcggacatcg acggcgacgg tctgggtgac
      901 gcgtgcgacg acgacgacga tgccgacggc gtgatcgacg cggccgattg tgcgccgcag
      961 cttcgcggcg tatcggcgcc gccgggaccg gtcggcgcga cgctgcggct cgccggcggc
     1021 agctcgacgc ggatcgagtg gaagcgtggg atccaggggc acgtgtcgaa cgtgttccgc
     1081 gggtcgatcg agtccggagc cgcctggagc tacgacgaga gctgcttcga cgccgagaca
     1141 ccgctcgaca gcaccgacga cgacgagatc ccggcgccgg gatcggcgtt ctactacctc
     1201 gtcaccgcgg ccaacgcctg cggccaggac gtgacgagcc tggcctcgac ccgcccgacg
     1261 atcgacccgc tggtcgaatg cctcccgcag ggcggtgatg cggacgccga cggggtgatc
     1321 gatctcgccg accattgccc gcaggacgcc gacccgtcgt tgatcgacgc cgacggcgat
     1381 ttcgtcggcg actcgtgcga tggttgcccg caggacgccg acgcggacca ggccgacgcc
     1441 gatctcgacg gtctcgggga cgcgtgcgat ggctgcaccg acaccgacgg cgacggcacg
     1501 ggcgatgcgg cgttctcgcc gacgacgtgc gcgatcgaca actgcggggc gatcgccaac
     1561 ccgacccagg ccgacggcga cgccgacggc atcggcgacg cctgcgacgc ctgccccgcc
     1621 gacgcggtca acgacggcga cggcgacggg tcgtgcgccg gaagcgacaa ctgcccgctc
     1681 gtcgccaacg ccggtcaggc cgacggcgac ggcgacggca tcggcgacgc ctgcgacgcc
     1741 tgtccggccg acgcgaccaa cgacgcggac gccgacggct actgcgccga cgccgacaac
     1801 tgcccgttcg tcgccaacgc cggtcagagc gacggtgacg gcgatgcggt cggcgatgcc
     1861 tgcgacgcct gtcccgatga cgcggtcaac gacggcgatg gcgacgggct gtgcgccgac
     1921 accgacaact gcacgaacat ccccaacccg agtcaactcg actcggacgg tgacggcgcg
     1981 ggcgatccgt gcgacatttg cccgttcaac gacgacccgg actgcacggt ctgcagccaa
     2041 ggggtcgacg acgacggcga cggcatctgc aacgacgacg tcgagctgac cgtcttctcc
     2101 ggctcgggcg agatcgtgct cgtgcaggcc ggcgcgacga tgcgctacct ggccaactcg
     2161 accgcgccag cggtcgacat gtcgtggact gccgacctgt tcgacgacgg tggctgggcc
     2221 gagggggttt acgggatcgg ctacgagacg aacacgggcg cggaagcgct ggcccagacc
     2281 gtcgtcgcga gcaacacggt ctcggtcttc acccgttcga ccttcgtgat cgacgacctg
     2341 gcgcgggttc gcaacctgtt cctcggcgcc gactacgacg acgcctacgc ggcctggatc
     2401 aacggcgtcg aggtctatcg ctcgccgcag gtgccgctcg gcccgctcgc ctgggacacg
     2461 cgggtcggcg gacacgaatc gagcaacggc gaccaacccg actacgggac gctggtcgag
     2521 atctccgcct cggttctgcg taacggcacc aacgtactgg cagtcgcggt ctggaacgcg
     2581 gtacccggtg gtcgcccgtc gtccgatctg gtgctcgccc cgcgcctttc gatcaatcac
     2641 tcgacgtcga cgacgatgcg ctacctggcc aaccaggtcg acccggcgat cggaaccgca
     2701 tggatctcac cggcgttcga cgacggcggt tggagcgacg gcaactacgg ggtcggctac
     2761 gacaccggat cgggtgcgct cggcctgatc aacacgtcgg tccccgccgg cacgttctcg
     2821 gtctacacgc gcgtgcggtt cgaagttgcc gacccgtcgg cgatcgtgcg gatggagttg
     2881 gggacggact acgacgacgg ttacgtcgcg tggatcaacg gcgtcgaggt ccaccgctcg
     2941 gccgagatgt cggcgggcac gcccagctgg aataccaacg ccggcttcca cgagtcgagc
     3001 aacgcgctgg cacccgacta cggagcgccg gtcgacctcg cggccgtcgg catcccggcg
     3061 ctcgtcgccg gtgagaacgt gctcgccatc ggcgtgtgga acaacggtgc tccggggtca
     3121 agcgacctgg tgctggtgcc gcaattgacg atcaacggcc cgatcgccga caactgcccc
     3181 ggcgttccca acgccgatca ggcggacgtc gacggcgacg gcacgggcga cgcctgcgac
     3241 ggcgacacgg acaacgacgg tcgtgccgac gcgctcgaca actgtcccgc ggtcgcgaac
     3301 cccggacaac tcgacggcga cggcgacgag gtcggagacg cgtgcgacgt ttgcccggcg
     3361 acagccgacc cgcaacaggt cgacgtcgac gaagacggca tcggcgacgc ctgcgacaac
     3421 tgtccgtccg acgcgaaccc cggccagagc gacggcaacg gcgacggtct gggcgacgtc
     3481 tgcgaccctg acgaggacgg tttggccacc ggtgacgaca actgccccgc gacgagcaac
     3541 gtcgaccaga ccgacagcga cgccgacgga cccggcgacg cctgcgactg cgacagcgcg
     3601 gatccgaccg tgtgggccac gcctggcgtc gtcgtcgatc tgcggttgag ccacgacggc
     3661 gggccgggac tgaccaccct cagctggacg cagccggcgg cacccggcgc cgacctggtc
     3721 cgtttcgaca cgctgcgctc gaccgacgcg accgacttcg tcggggccgg cgcatgcatc
     3781 gataccgacg gactcgacac gacggcgacg gacgaactga caccgccacc gggcacggcg
     3841 gcgtacttcc tcgtgcgtag cgagaacgac tgcccggcga gcaacgtgaa cgtcggacgc
     3901 cgctcggacg gtcagccgcg gccgacgccg acctgcccct gacgcgctgc gcgaacgccg
     3961 tccgccggcc cacacgttgg ggtaggatcg gccggatgag cggagagccc tcgcccgacc
     4021 gtgtcgcgcc cgaggccaag accgggctcg acccgtgggt cgagggcgag ctaccgccgc
     4081 cccccgaacc gacgggtctg gcgtggctcg gtgtcgtcgg cccgggcgtg atcgtgctcg
     4141 gcgcctcgat cggcagcggc gagttcctgc tcggacccgc ggcgttcgtc aagtacggcc
     4201 tgagcctgct gtgggtcacg gggctcgcgg cctttctgca gacggtgttc aacaccgagc
     4261 tgatgcgcta cacgctggcc accggcgagc cgatcttctc cggcttcatg cgcacgcgcc
     4321 ccggcgcacg tttctggggc ttcgtctact cgttgctcta cgtgctccag gtcggatggc
     4381 cgggctgggc cggtgccgcc gcgggcgcgg tcttcttcct gttcgccggg agggtcgccg
     4441 cgacggggga cgagcacgcg gtctacctga tcggcgtcgg cacgttcgcg gcgtgcgtgg
     4501 cgatcctggc ggtcggccgc cgtatcgagc gcacgctcga gatcctcaac tggatcctgg
     4561 tggcgatgat actcggcggc ttcgtcgggt tggccgtggc gctcgtcgcg ccgcagacct
     4621 ggctcgccgc gatcgccggt ttcatcggct tcgctccggc gaccggtcgc ttccagttga
     4681 ttcccgccga tgccgatttt tttctgatcg gggcgttcgc cgcctactcc ggcgcgggcg
     4741 ggatgatcaa cctgacgttg agcaactggg cgcgcgacaa gggctacggc atggccgccg
     4801 gcgcaggcta catcccggcc gcgaccgccg gacggcacgt ccacctgacc cacaccggct
     4861 ttcgcttcga gcccgacgct gcggcgctcg agcgctggcg cgggtggtgg cgcgtcgtcc
     4921 gtgccgacca gtggggtgtc tatttcatcg gagcgatcgt cgggatggcg ctgcccggga
     4981 tgatctacgt caccttcctt cccgccggca gcgacatccg cggtctcggc atcgccgccg
     5041 ccctggccga tgcgttgcgc gagcgcgcgg caccgtggat cggtatcttc gtcgcactgc
     5101 tcggcgtgtg ggtgctgttc aagacgcagc tcgacatcct cgacggcgtg acccgcgcgc
     5161 tgaccgacat cctgtggacg ggcagccgca ggctgcggcg aaacccgaac gcggacgtcc
     5221 gcgcggtcta ctacggcgtg ctgctcgcgc tgtcgctgtg gggcgtcgtc gcgttgcggt
     5281 tgacgcagcc gatcgtgctg ctgcagatcg gcgcgaacat ggccgggatc gtgttcatcg
     5341 tgacgtcgat ccacctgttg cgggtcaata cgacgctgct gccgcccgag ctgcgccctc
     5401 cgacctggcg acgcctcgtc ctgatcggcg tcgcggtgtt ctacgggtcg ttcgtttcgc
     5461 tgtggctcgc gagcctcgtc ggtcgatccg gcaccgcgct cggctgaagc tcggcgcgcg
     5521 gtcatcgtcg ccggcctcgc tacatctcgc gcagaaactc tccggtccgg taatcgtaga
     5581 cccggaccgc gatgcgaacc cgcggcgagc ggctgtcgta gaacgacacc cgcacgacct
     5641 tcttctcgct cgtcggtcga tagaccagcc agtcgccggg gccgacgtcc tgttcgacga
     5701 ccgagcacga tccgggcgcc ggctggtgac cgggcggccg acccggatgc cagacgcggc
     5761 actggccggc gggcggaaag tgacccggtg ggatcccgag ccgtgagggg gtcgagtggc
     5821 cggggccggc tttcggcggc ggctcgggga ccgtgctgct gccgccgcgc gcctcgatgg
     5881 acaacgaggc gcagccggtc gccgcgaaga ccgtcatgca caggagagcg agactggtgg
     5941 tggctcgcac gagagtaccc tcctcccccg ccggaaacct aacgtcgagc gagcggcggg
     6001 gcaaacccgt cgcgatctca aaatcgaagc ccgagtttcg atatcgttcg tcggacgacc
     6061 ggacgaatcg gcgaagcggc cggtgggcgg actctctcgc gggaaaaccg tgcctggcac
     6121 cgtcgttgct cgaccgtcgg accgagcgac catgggttcg ttttcgttcc cctgacggcc
     6181 gtggcccgat gccgcgccgc cgggcgggaa tggcgggagg catgatgcac gagcgacaat
     6241 cgaaagagac gtggctggtt ctggcgttgg cactaccgct gatcgcggtc gggtgcgccg
     6301 gcgagccggc cgccgagatc gacgcggcca gcgcggccct cgaatccgcc cggctcgcag
     6361 aagccgaagt ctatgcgccg gaggcgatgc aaaccgccga ggctgcacac gccgagctgc
     6421 aaaccgagct cgccgcgcag aaggagcggt tcgcgttggg acgctcctac gacaagaccc
     6481 gcgagctcgc cgccgatgcg cagcgcgcgt cggaggaggc ggtcggggct gcccacgccg
     6541 gtaagctgat ggcgcgtgag gaggcgatga acatgctcgc cgagctgcgc acgtcgctcg
     6601 acgaagtgga gaccatgctg gacaacgccc cgcagggcaa gggctccgcg gcggatctcg
     6661 cggcgctccg aggcgacctc ggcgccgtcg acgtcgcact gaccgacatc gaacaggcca
     6721 tatccgccca gcgcttcatc gatgcggtcc agaaggcaca ggcggcgatg gcgagcaccg
     6781 ccgagattcg cagcgagatc gagcgggcgc aggaggcgca gcgcgctgcg aaatcgcgtc
     6841 gcggttgatc ggatcggcgg cgccgagggc gacgcggcgg ccaggtcgag cgaggtgctt
     6901 gatcgttttg ggctccggcg tctcggttct gctcgcggtt tccgtgcacc acgcgttcac
     6961 cacgcgtgcc gcgccgcctg ctttgctcgc gctcgaggca cgccgcgcgc tcgatcgtgc
     7021 cgacgacctg ggcgcccgag agtgggcgct ccggccgctg gtcgaagccg cgtccgcgct
     7081 ggacgccggc cgcgccgagg aacgccgcca acgatcgcgt tggctgccgt tccgcgattt
     7141 cggtgaggcc cgcgcgagtt atcggcgggc gatcgacgcc gcgaagcggg ccgaggaagc
     7201 cgcggccgaa gcgaacgcgg tcatgatcgg ccgcgtcgag acgcgactgg ccagggtgga
     7261 ggagatcgtc gggcaggtcg tcggactcac ccgcggcatg agactcgagc aggccgagag
     7321 ccgttcgttg cgagtcgccc ggctgaggct ctccgaggcg cgggtccagg ccgagtccgg
     7381 acgctaccga tcggcggacc gtctcgccgg cgaagcgctg gcccacacac gccgaggcgt
     7441 cgactgcctg cgggatcgag cggcgcgcta cgtcgatcgg aaccacgtgc gccgctggcg
     7501 cgcctgggaa gcggagacga tcgtctggtc gcgtcggcac cgagcggccg cgatcgtggt
     7561 caacaaggaa cacaaccgtc tgaccctcta cgttgccggg aagcccgagc gcgatttcca
     7621 cgccgacctg ggtcgaagcc acttcgacgc caagctgcgc gcggccgacg gcgcgactcc
     7681 ggagggtcgc tatcggatca cgcgcaagaa gggcaagggc cggtcgacgt actacaaggc
     7741 gctggagctg gactacccga acgaggacga ccgtcggcgt cacgcggagg ccgtcagcaa
     7801 cggtcgtgtt ccgcgtcggg tgggcgcggg cgactcgatc gagatccacg gcgccggagg
     7861 ccgggacgac gactggacac agggctgcgt cgcgctgtcc aacgacgaca tcgactacgt
     7921 gttcgacgcg gtccgggtcg gcacgccggt gacgatcatc ggcggggatg gtggcggagg
     7981 acggttctcc tccttggccg cgcgcttggc cgacgcgcgc gaagagctcg atgggttcgt
     8041 acggaactga atcgcccccg cgtgcttggt ccgctcgcgc gcgaccccgc gtgacgatcc
     8101 ggctggtgcg acgcggcaag gcgctctccg tggcggcggt tgcgctcgcc gtcgcggtgc
     8161 tcggcgtgtc gacgagcggc tatcgctatc gggtcgaggc cctgccgttg acccgcaccg
     8221 aacaggcgct cccgaccgag acggccgcgc tccaggcgtt cgtcgccgac cagcggcgcg
     8281 agcaggcacg gctcgaccgg caactccgcc gcgcctcgcc ccgcagcact tatatcgtca
     8341 tcgaccgcac acacaaccgc ctgcaactgc ggcgcaacgg cgagacgatg cgtgaggcga
     8401 cctgctcggc cggttccggc atggtgctcc gtgacgaggc gcgtgatcgc gagtgggttt
     8461 tcgacacccc gcaggggcgg ttcgaggtgt tacgcaagat cgaagacccg gtgtggaaga
     8521 aaccggattg ggccttcatc gaacaggggc agccgctccc cgccgatccc ggtgaacgca
     8581 tcgagtacgg tgtcctcggc gagtacgcgc tgtacttcgg cgacggctac atgctgcacg
     8641 gaacgctcta cgaacgtctg ctcggccgca gcgtcaccca cggttgcgtt cgactcggtc
     8701 gcgaagatct gcgcgcggtc taccacaact ccgccgtggg cacgccgatc ttcatctatt
     8761 gaggccggga tgaggatgat tcgacgcaac ccgatcgcga cgaccgcggt ccttctcccg
     8821 ttgctcgggg tgggtttcct cggcttcgcc gctggggtgc ccgacgacga cgacggcgcg
     8881 cggacactgc gcgagcgcgc gctgcgcctc gtccacggga acacctgccg acgcgcggaa
     8941 gcggagttgg tcgagggcga acgcttctat ctggtcctcg acatcgatcg acgctcgctg
     9001 agcctgaagc tggggggtgt aatgctgcat gagctgccga tcgagtcgat cgaggtcggc
     9061 cggccgtatc gctttttctt cgaacacgac gggccgaagc cgattcgcgc ccggctccat
     9121 cggcaatcca gcctgtcgcc cccacgcctg ccgctgaggc tcccggtcga gattccggaa
     9181 gaggccgacg tcgagacgca aacgatcatg ctcccacccg agcccgaaac cgcgatcgcg
     9241 gtaccgcgga gattcttcat tcgctaccga gaaggcgtgg tgctcgagtt ccgcaccggt
     9301 cggcgcgacc tgggacagcg catcaacgac cgcgtcgcgg cgctgttccg cgggccggag
     9361 ctgcgcttgc gcttggtcct cgagcgtcga gcggcggaga tcttctatcg ttcggtgccc
     9421 cgcgaaaccg acctgctcgt gctgtgatcc gacgccccac cgccggtgca gcggaacggc
     9481 ggcacttgcc ggacggtgcc caagctggca gcatgtgtcg ctcggcgata cgactgacgt
     9541 gcgacggctc ggtgccggga ggtcggaagt gaggaagatc gggctctttt tgtcttgtct
     9601 gctgatcagt ggggtcgcgg tggcagaccc ggccaccttc gtcgagatcg cgttgatcga
     9661 cggcggcgag aacggtcgca acggctcggg cgatcagttc ggattcgagg ccaccggcga
     9721 ctacggcttc aacgagcatt ggtacgtcgg cggcgccctc ggtcagttcg aacgcgactc
     9781 ttccggcggc gacatcgaga acacctattt caatgtgcac ggcggctacg tcgtgcccgt
     9841 ggccgaccca accggcgtca acctcgaggc cggtctgtgg ctcggggatc aggacaatcc
     9901 cggcgacgag gatacggatc cccgggcgtt cgagatcaag gccggtgtca accactcgct
     9961 gtcggacaaa ttcggcatct tcggaacgct cgcttgggtt cacggagacc tcgacacgcc
    10021 tcaggacagc gacctgcgca actacgtttg gtcgctcggc ggagcctatt cgatcagtga
    10081 gcgcgtttcg atcaacctga agatcgtcaa cggcgtcaac gg
//
LOCUS       DASPGN010000313         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1343475, whole genome shotgun sequence.
ACCESSION   DASPGN010000313 DASPGN010000000
VERSION     DASPGN010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343475"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..513
                     /locus_tag="VD788_09075"
     CDS             277..513
                     /locus_tag="VD788_09075"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HXV76459.1"
                     /translation="MSQDNVERVIGRLLTDEAFRNRFCEDPETALAGLVEDGMTLNPC
                     EVRALAALDARWIFGFADAVDPRIQKTDLSGGRR"
     gene            510..2102
                     /locus_tag="VD788_09080"
     CDS             510..2102
                     /locus_tag="VD788_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV76460.1"
                     /translation="MTPAARTAVLLLCALLPAPPARPAPTSSAEGYPLTLLEAIESSL
                     EKNERIVISRESLDSASEAVRGARGAYDPFLEIEAGWRESTPPLNSAFSGAPPGELSP
                     TSESVDAGAALHQLLSTGGELSVRTGVVRATTDGSFTFLSPAYDTFVGVELRQPLLRD
                     RAIDPFRLNVRLAASERELSDALLRVDVTDTVAAVERAYWIYVALREAVAVREDAIRL
                     AEEQLEETRARIDSGIAPDTEVAQPLAELERRRGDLLATQEAVVRAQNALKLLILDDS
                     DDALWTGEIVPVEAVVVDPVEVDIERAIELALAARPEIEAAAAVIDQREIESRFASNQ
                     VQPALDAVVSYDRFGLAGSLNPAASTIPGLPTTVPPDLEGSLGRSYGVLGDGDLDDVR
                     AGLVFSIPLGNRSAKSAAAIARNGERQAAAELTRTRKIVRAEVLDAAAAVETTYQRVE
                     AARAALDAARVQLEAEQDRFAAGLSTNFLVLTRQNDLEERRIDEIAARTDYLNARTAM
                     GRATGTLLDDLGIDMVDIVEAQ"
     gene            2140..>3128
                     /locus_tag="VD788_09085"
     CDS             2140..>3128
                     /locus_tag="VD788_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV76461.1"
                     /translation="MLIMLVAFLAVVGVIGFVKFQQIQAAIAQARSFQPPPTAVTTIV
                     AEEVSWRTTLRAIGTIAAVHGVTVSADLPGIVARISFESGSRVEAGDELVRLDTRQER
                     AQLEAAEAERELARLNLERMEELRQKGVVSQAELDAADARFKTAEAQLGQIRATIERK
                     TIRAPFTGILGIRQIDLGQYLAGGDPVVPLQSLDPIHADFSVPQQDIARVGPGTVVRV
                     EVEGSDIEREGRVTAVDSIVDEATRNVRVQATLDNRRGDLRPGMFVETHVVDETPVPV
                     VTVPASAISYAPYGDSVFVVTELTGPDGATYRGVRQQFVKLDGTQGDRVAVVSG"
BASE COUNT          486 a         1094 c         1110 g          438 t
ORIGIN      
        1 aacaagtcga aggccgcgcg gctgctcggg ttgagccgct cgcagctcta ttcccgactc
       61 gagaagtacg ggatcgactg aaccccgggc ccgcgtcgtc cgcgcgcatt cccgcaacct
      121 gtcgattatc ggacagcagc cgtccggcac gccccgagcg gcccgtcgtg cgccccgcac
      181 gacaaccggc accgaatgaa cgagttggac gctgcgtccg ccaccctgac gggctggcac
      241 gcgccttgtt acaccgactc gccgggaggc gcgaccgtga gccaagacaa cgtcgaacga
      301 gtgatcggac ggctgctgac cgacgaggcg ttccgcaatc gcttctgcga ggatcccgag
      361 accgcgctgg ccggcctggt cgaggacggg atgacgctca acccgtgcga ggtgcgggcg
      421 ctcgcggcgc tcgatgcccg gtggatcttc ggcttcgccg acgcggtcga cccgaggatc
      481 cagaagaccg acttgagcgg agggagacgg tgacgccggc ggcgcggacc gcggtgctgc
      541 tgctgtgcgc tctgctgccg gcgccgcccg ctcggccggc gccgacgtcg tcggcggagg
      601 gctacccgct gacgctgctc gaggcgatcg agagcagcct cgagaagaac gaacggatcg
      661 tcatctcccg cgagtcgctc gattcggcga gcgaggcggt gagaggggcc cgcggcgcct
      721 acgacccgtt cctcgagatc gaggccggct ggcgggagtc gacgccgccg ctcaattcgg
      781 ctttctccgg cgcccctccg ggcgaactgt cgccgaccag cgagagcgtg gacgccggcg
      841 ccgcgctgca ccagctgttg tcgacgggcg gcgagctgtc cgtgcgcacc ggtgtcgtcc
      901 gggcgacgac cgacggatcg ttcaccttcc tgtcgccggc ctacgacacc ttcgtcggcg
      961 tcgagctgcg gcagccgctg ctgcgcgacc gcgcgatcga cccgtttcgg ctgaacgtcc
     1021 gactcgccgc cagcgagcgg gagctgagcg atgcgctgct gcgggtcgac gtgaccgaca
     1081 cggtggccgc ggtcgagcgc gcctattgga tctacgtcgc gctgcgtgag gccgtcgccg
     1141 tgcgcgagga cgcgatccgc ctggccgagg agcagctcga ggagacgcga gcgcggatcg
     1201 acagcggcat cgcccccgat accgaggtgg cccaaccgct ggccgagctc gaacggcgtc
     1261 gtggcgacct gctggcgacg caggaggcgg tggtgcgcgc acagaacgcg ctcaagttgt
     1321 tgatcctcga cgattcggac gacgcgctgt ggacgggtga gatcgtgccg gtcgaggccg
     1381 tcgtcgtcga cccggtcgag gtcgacatcg agcgggcgat cgagctggcg ctcgccgcgc
     1441 gcccggagat cgaagcggcg gcggcggtga tcgaccagcg cgagatcgag agccgcttcg
     1501 cgagcaacca ggtgcagccg gcgctcgatg ccgtcgtctc ctacgaccgc ttcggcctgg
     1561 ccggctcgct caacccggcg gcatcgacga ttcccgggct gccgacgacc gtcccgcccg
     1621 acctcgaggg cagcctcggc cggtcctacg gcgtgctcgg cgacggcgat ctggacgacg
     1681 tacgggccgg gctggtcttt tccatcccgc tcggcaaccg ttcggcgaag tcggccgcgg
     1741 cgatcgcgcg aaacggcgag cgacaggccg cggccgagct cacccggacg cgcaagatcg
     1801 tccgcgccga ggtgctcgac gccgccgccg ccgtcgagac gacctaccag cgcgtcgaag
     1861 cggcgcgcgc cgcgctcgac gccgcacgcg tccagctcga agcggagcag gaccgcttcg
     1921 cggcggggct ttccaccaac ttcctcgtgc tcacgcggca gaacgacctc gaagagcgtc
     1981 gcatcgacga gatcgccgcg cgcacggact atctcaacgc acgcaccgcg atgggacgcg
     2041 ccaccggcac gctgctcgac gatctcggaa tcgacatggt cgacatcgtg gaggcacaat
     2101 gacgacgaag gttacaggag tagtccgatg atgaagcgca tgctcatcat gcttgtcgcg
     2161 ttcctcgccg tcgtgggcgt gatcggcttc gtcaaattcc agcagatcca ggccgcgatc
     2221 gcccaggcca ggtcgttcca gccgccgccg acggcggtga cgacgatcgt cgccgaggag
     2281 gtgtcgtggc gcaccacgct gcgcgcgatc ggcacgatcg ccgcagtcca cggcgtgacc
     2341 gtcagcgccg acctgcccgg gatcgtcgcg cggatctcgt tcgaatccgg ctcgcgggtc
     2401 gaggccggcg acgagctggt ccggctcgac acgcgccagg agcgcgcgca gctcgaggcg
     2461 gccgaggccg agcgcgagtt ggcgcggttg aacctcgagc gaatggagga gctgcgacag
     2521 aagggcgtcg tcagccaagc cgagctcgac gcggccgacg cacggttcaa gaccgccgaa
     2581 gcacagctcg ggcagatccg cgcgaccatc gagcgcaaga cgatccgcgc gccgttcacc
     2641 ggcattctcg gcatccgcca gatcgacctg gggcagtacc tggccggcgg cgacccggtt
     2701 gtcccgttgc aatcgctcga cccgatccac gccgacttct ccgttcccca gcaggacatc
     2761 gcgcgcgtcg gccccggcac cgtcgtccgc gtcgaggtcg agggcagcga catcgagcgc
     2821 gaaggccggg tgaccgcggt cgactcgatc gtcgatgagg cgacgcgcaa cgtccgcgtg
     2881 caggcgacgc tcgacaaccg gcgcggcgac ctgcgtcccg ggatgttcgt cgagacccac
     2941 gtcgtcgacg agaccccggt tccggtggtg acggtgccgg cgtcggcgat cagctacgca
     3001 ccctacggtg actcggtctt cgtcgtcacc gagctgaccg ggccagacgg tgcgacctac
     3061 cgcggtgtcc ggcagcagtt cgtcaagctc gacgggacgc agggcgatcg ggtggcggtc
     3121 gtctcggg
//
LOCUS       DASPGN010000314         6896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_173092, whole genome shotgun sequence.
ACCESSION   DASPGN010000314 DASPGN010000000
VERSION     DASPGN010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6896
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173092"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VD788_09090"
     CDS             complement(<1..659)
                     /locus_tag="VD788_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76462.1"
                     /translation="MHNQTARSIAEPRAETPLRPHAGTPRRRLLADRLARYSVIGGGL
                     VIIASILGILVFILIEVAPLVGGADVAGGHVIEPGGPRAAAMITDDYRTRLALLSDDG
                     TLRVVDAEDGSIVLERKLLPDTDGATPDGDVVLAATGAPGAAHFVAATRGGRVIVQPV
                     DWQVDFVGQDRRVTAELPPPVVLVLDPAGGRLGAFTAARDDDGTTTAAATLEGGQIAI
                     AR"
     gene            complement(695..1678)
                     /locus_tag="VD788_09095"
     CDS             complement(695..1678)
                     /locus_tag="VD788_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HXV76463.1"
                     /translation="MNQRPSALAILLVVGSAAGASSPRSAEAVQVDPSLRPYQTTSGV
                     SGNLDSIGSDTLNNLMTLWAEGFRKTYPNVRIQVEGKGSSTAPPALIKGTAQIGPMSR
                     AMKATEIDEFERAFGYEPTQIRVAIDALAVYVNKDNPLDKLTLEQVDAVFSSTLKCGH
                     GGSIDRWDQLGLSGSWSGKPISLYGRNSASGTYGYFKTEALCKGDFRDIVKEQPGSAS
                     VVQGITEDLYGIGYSGIGYKTSGVRTLDLAKGAGEPYFSTDPETVLAGDYPLSRFLYL
                     YVNKAPNRALDPLAREFLKFVLSRDGQETVVKDGYLPISTTIAERELAKLD"
     gene            1786..2553
                     /locus_tag="VD788_09100"
                     /pseudo
     CDS             1786..2553
                     /locus_tag="VD788_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            2849..3058
                     /locus_tag="VD788_09105"
     CDS             2849..3058
                     /locus_tag="VD788_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76464.1"
                     /translation="MAHFEKMLYDNARLVPAYLDGYQASGEPGFATIARELCDYLLRE
                     MAAPGGGATASPPAGTSRVAACCGS"
     gene            3043..4029
                     /locus_tag="VD788_09110"
     CDS             3043..4029
                     /locus_tag="VD788_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76465.1"
                     /translation="MLRLVTPLEQLAADGEVRSSLEAARGTLDRVRARRPPALRDEKI
                     LTAWNGLALSALARAGFVLEREDYLAAARRTATFLLDELRVDGRLRRSHHEGQARHDG
                     YLDDYVMLIAGLLDLYESTGEIGWLTHAVELQQTLDRDFWDADVGGYYMTSDDHEPLL
                     ARDKPAYDGAMPSGNAVGLWNLVRLHALTLDDAYRARGARLLAAFARTLAAQPGAVSE
                     MLLAVQFELADPLEIVIVTSESRAEADPYLDVLRRRYQPFAVLAVTVDGARREAESRR
                     LPPLVGKTARNGSTTAYVCRRAVCRLPAGDVEAFVSELDRALGDEGPPTLKK"
     gene            4146..4475
                     /locus_tag="VD788_09115"
     CDS             4146..4475
                     /locus_tag="VD788_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXV76466.1"
                     /translation="MAKADAPKKSMTKSEILNAISEETELPRKEVSAVLDSLSHQISR
                     SLGRRGPGVFTLPGLVKIEKRKIPARKARRGVPNPFKPGELMDVPAKPASTKVKVRPL
                     KNLKEMV"
     gene            complement(4537..4938)
                     /locus_tag="VD788_09120"
     CDS             complement(4537..4938)
                     /locus_tag="VD788_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HXV76467.1"
                     /translation="MNSRLTVMALLLLVLGCCACSGSGRTDEQSGEGTPGAAVVSFFD
                     HLNAGHLDAARELYTTDARVVVEDNEVFRGWADEVTQQGTIESVEILASTVTDDTAAV
                     QFAIVFADGTSREHTVECLNEDGTWRLGAIL"
     gene            complement(4935..5924)
                     /locus_tag="VD788_09125"
     CDS             complement(4935..5924)
                     /locus_tag="VD788_09125"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HXV76468.1"
                     /translation="MQIYETILDTLGNTPLVRLGRFCPGDAIVAAKIESFNPGSSVKD
                     RIGPALVEAGERSGKLRPGGTIVEPTSGNTGVGLAMVAALKGYKLVCTAADKIPAEKV
                     TLLEAFGAEVITCPTEVPADDPRSYYKVAERIRDERGAYLPYQYYNQANPEAHYRSTG
                     PEIWRQTDGRITHWVAGIGTGGTISGVAKYLKEQSPDVRVIGVDTVGSVYAYYHEHRR
                     MPPADRIHQYLIDGIGEDFMPETVWWDQIDEVVTVDDRAAYDATLQLARREGILCGSS
                     GGAAAEGARRVAAGVGRDAVVVTLFPDSGERYLSKLNQKWLREKGLIDDGGER"
     gene            complement(5929..6495)
                     /locus_tag="VD788_09130"
     CDS             complement(5929..6495)
                     /locus_tag="VD788_09130"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HXV76469.1"
                     /translation="MTDLPAYRPLIEMLDRAMVEDDVEALTRRLADGLHELASQRRIA
                     LPESVRSVNPSTYARRLLHRCPERGYTVVAMTWGPGQQTQLHDHAGMWCVECVIEGRL
                     EVVQYDLLERVGDRCRFERRGSVHAGVGDSGCLIPPFEHHVLRNALPADRSVTVHVYA
                     GEMERCNLYLPAEDGWWTRTATELAYHD"
     gene            6620..>6896
                     /locus_tag="VD788_09135"
     CDS             6620..>6896
                     /locus_tag="VD788_09135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV76470.1"
                     /translation="MDRSSVRFLGTGTAFNHAGRRSQSFLVRAAGQSPFLVDAGPTVM
                     VPLMQEGSAYDEIDRLFVTHLHGDHTAGWPFLLLHLVILARRRRPFDV"
BASE COUNT         1077 a         2354 c         2335 g         1130 t
ORIGIN      
        1 cgcgcgatcg cgatctgtcc gccttcgagc gtggcggcgg cggtcgtcgt gccgtcgtcg
       61 tcgcgtgccg cggtgaaggc accgagtcgg ccgccggcgg ggtcgagcac gagcacgacc
      121 ggcggcggca gctcggcggt cacgcgtcga tcctgaccga cgaagtcgac ttgccaatcg
      181 acgggttgga cgatgacgcg cccgccgcgt gtcgcggcga cgaagtgtgc ggcgcccgga
      241 gcgcccgtcg ccgccagcac cacgtcgccg tcgggcgtgg cgccgtccgt gtcggggagc
      301 agcttgcgct cgagcacgat cgagccgtcc tccgcgtcga ccacgcgcag cgtcccgtcg
      361 tccgacaaca gtgccagccg ggtccggtaa tcgtccgtga tcatcgccgc cgccctgggg
      421 ccaccgggct cgatcacgtg gccgcctgcg acatcggcgc caccgacgag cggcgcgacc
      481 tcgatcagga tgaagaccag gatgccgagg atgctggcga tgatcaccag accaccgccg
      541 atcacgctgt atcgggccag ccgatcggcc agcaggcggc ggcgcggggt gccggcgtgc
      601 ggtcgaagcg gcgtctccgc tcgcggttcg gcgatcgagc gtgcggtctg gttgtgcatc
      661 cggtcccggg atcccgtcgg ccgtcgcgcc gagctcagtc gagcttggcc agctcgcgct
      721 cggcgatcgt cgttgagatc ggcaaatacc cgtccttgac caccgtttcc tgtccgtcgc
      781 gcgaaagcac gaacttgaga aactcacgcg cgagcgggtc gagcgcgcgg ttgggggcct
      841 tgttcacgta gagatagagg aagcgggaca gcgggtagtc gccggccagc accgtctcgg
      901 gatcggtcga gaaataaggt tcccccgcgc ccttggccag atcgagcgtc cgcacgcccg
      961 aagtcttgta gccgatcccg ctgtaaccga tcccgtagag atcttcggta ataccctgga
     1021 cgaccgaggc cgatcccggc tgctccttga cgatgtcgcg gaagtcgccc ttgcacaggg
     1081 cctcggtttt gaagtacccg taggttccgg aggccgagtt gcgcccgtac aggctgatcg
     1141 gcttgcccga ccacgagccg gagaggccga gttgatccca gcggtcgatc gatccgccat
     1201 gcccgcactt gagcgtcgag gaaaagaccg cgtcgacctg ctcgagcgtg agcttgtcga
     1261 ggggattgtc cttgttgacg tagaccgcca aggcgtcgat cgccacgcgg atctgcgtcg
     1321 gctcgtaacc gaacgcgcgc tcgaactcgt cgatctcggt ggccttcatc gcgcggctca
     1381 tcggcccgat ctgggccgtg cccttgatca gcgcgggcgg agcggtcgac gagcccttgc
     1441 cctcgacctg gatccgcacg ttcgggtacg tcttgcggaa gccctcggcc cacagggtca
     1501 tcaggttgtt gagcgtgtcg gagccgatcg agtcgagatt gccagagacg ccgctcgtgg
     1561 tctggtacgg tcggagtgac gggtcgacct gcaccgcctc ggccgaccgc gggctcgagg
     1621 ccccggccgc cgagccgacc acgagcagga tcgcgagggc cgatggtcgc tgattcacct
     1681 ggatcctccc ctcggcgacc cggttcgacg gggccgagca tgacggcaac cacagctcgt
     1741 tgtaaagaaa ccgtatgatc tgggtgttag gacaatgtta gctttatgtt aggagcctca
     1801 ttcgacccga atcccgcgcc tcgtcccgat cgtctcgccg gtcgaccgcc ccgccttcgc
     1861 accgtcctcc gcgccatcgg aacggcggcg tcggccggca ccgcgctggc gggagccgcg
     1921 ggcgagggac gggcgcccgc cgtggcggcg ccgccgggcg cccggtcgta cacgccgacg
     1981 ctgtcccgcg agctcgcggt tcggctcgcg tcgttcgggg agtcctaccg accgcggacg
     2041 cgccatctca ccgcggacgg atcgccgctc tacaccaatc gactggtacg ggaatcgagc
     2101 cggtacctcc ggcagcacgc ccacaatccg gtcgactggt atccctgggg tgacgaagcg
     2161 ttcgacaccg cgggccgcct cggacgaccg gtgctgctca gcatcggcta gtcgacctgc
     2221 cactggtgtc acgtgatgga gcatgagtcg ttcgaggacg aggagatcgc ggagttcatc
     2281 aaccggaact atgtcgcgat caaggtcgat cgcgaggagc ggcccgaact cgacgcgatc
     2341 tacatgcgcg cggtggtttc gctggtcggc cggggcggtt ggccgctgac ggtgtggctg
     2401 accgcggagg ccgagccgtt ctacggaggg acctacttcc cccgcgcgac ggggaccggg
     2461 gtcagcgcac cgggttcctt tccttgttgg agaacctgga tcggctctac cgggcggagc
     2521 cggaccacgt cgcacggcgc gcgcgcgaga tgacggccct gttgcgcggc tcggcgcagc
     2581 cgctgccggg ggagaccggc gacccggaga cgctcgtccg tcgggcgatc ggcgactacg
     2641 ccgcgcggtt ccacggggtc gccgggggcg tgagcggggc gcccaagttc ccgacggact
     2701 ttcccgttcg gctgatgctg cgggagtccg tgcggacggc cgacgcggaa ccggcgacga
     2761 tggcgctgcg gacgttgcgc cggatggccg aaggcggaat ctacgaccag atcggcggcg
     2821 ggttccaccg ctatgcgacg gacgacgcgt ggcgcacttc gagaagatgc tctacgacaa
     2881 cgcgcggttg gtgccggcct atctcgacgg ctaccaggcc tcgggcgaac cggggttcgc
     2941 cacgatcgca cgcgagctct gcgattacct gctgcgcgag atggcggcgc cgggcggcgg
     3001 cgctacggcg tcaccgccgg cgggaacttc gagggtcgca gcgtgctgcg gctcgtgacg
     3061 ccgctcgagc agctggcggc cgacggcgag gtgcgcagct cgctcgaagc cgcccgcggg
     3121 acgctcgacc gcgtgcgagc gcggcgtccg ccggcgctgc gcgacgagaa aatcctgacc
     3181 gcgtggaacg gtctggccct ttccgcactg gcgcgtgccg gcttcgtcct cgagcgcgag
     3241 gactacctcg cggcggcacg ccgcaccgcg acgttcctcc tcgacgagct acgggtggac
     3301 ggccgattac ggcgcagcca ccacgagggg caggcgcgcc acgacggcta cctcgatgac
     3361 tacgtcatgc tgatcgccgg actgctcgat ctctacgaaa gcaccgggga gatcggctgg
     3421 ctaacccacg cggtcgaact ccagcagacg ctcgaccggg acttttggga tgccgatgtc
     3481 ggcggctatt acatgacgag cgacgaccac gagcccttgc tcgcgcggga caagccggcc
     3541 tacgatggcg cgatgccgtc gggaaacgcg gtcgggctgt ggaacctcgt tcggctccac
     3601 gcgctgacgc tcgacgatgc ctaccgtgcc cgcggcgcgc gtctgctcgc ggccttcgcg
     3661 cggacactcg cggcgcaacc gggagccgtg agcgagatgc tgctcgcggt ccagttcgag
     3721 ctggccgacc cgttggagat cgtgatcgtg acctccgaat cgcgcgcaga ggccgatccc
     3781 tacctcgacg tgttgcgccg ccgctaccag ccgttcgcgg tgctggccgt caccgtcgac
     3841 ggcgctcggc gcgaggcgga gtcgcgccgc ttgccgccgc tggtgggcaa gacggcacgg
     3901 aacgggtcga cgacggccta cgtctgccgg cgagccgtct gccgactccc ggccggtgac
     3961 gtcgaggcgt tcgtcagcga gctcgatcgg gcgctgggcg acgagggccc tccgaccctg
     4021 aaaaaatagg ggtttggtga cgatcggcga ttgattcgta cgacaaaagc cgatagacta
     4081 cgcaatcggt tcacagtgcc ggtgcttcgc accactcaca cccacgaagg gaggattgac
     4141 aagagatggc caaggcagac gccccgaaga agtcgatgac caagagcgag atcctcaacg
     4201 cgatctccga ggagacggag ctcccgcgca aagaagtcag cgccgtcctc gattcgttgt
     4261 cgcatcagat ctcccgcagc ctcggccgcc gcggccccgg cgtgttcacg ctccccggtc
     4321 tggtcaagat cgagaagcga aagatcccgg cccgtaaggc acgccggggt gtgccgaatc
     4381 cgttcaagcc gggcgaattg atggacgtgc cggccaagcc ggcgtcgacc aaggtcaagg
     4441 tccgtccgct caagaacctg aaagagatgg tctgagaagc tgatcccgac gacccgcccg
     4501 gccgacggtc atgggtcccc gggcgggttc cctccgctac aggatcgccc ccaacctcca
     4561 ggtgccgtcc tcgttcaggc actcgaccgt gtgttctcgg ctcgtgccat cggcgaagac
     4621 gatcgcgaac tgaaccgctg cggtatcgtc ggtcaccgtc gacgcgagga tctcgaccga
     4681 ttcgatcgtg ccctgctgcg tgacctcgtc ggcccagccg cgaaagacct cgttatcttc
     4741 gacgaccacc cgcgcatccg tcgtatacag ctcgcgtgcc gcgtcgaggt gacccgcgtt
     4801 gaggtggtcg aagaacgaga cgaccgctgc gcccggggtg ccttccccgg actgctcgtc
     4861 cgtccgcccc gaacccgagc acgcgcaaca gccgaggacc agcagcaaca gcgccatcac
     4921 cgtcaagcgt gagttcatcg ctcgcctccg tcgtcgatca gacctttttc ccgtaaccac
     4981 ttctggttca gtttcgacag ataacgctcg ccggaatcgg ggaacagcgt cacgacgacc
     5041 gcgtcgcgac cgacgcccgc cgccacgcgc cgggcaccct cggcggcggc gccgccggag
     5101 gagccgcaga ggatcccctc gcgccgcgcc aattgcagcg tggcgtcgta agccgctcga
     5161 tcgtcgaccg tcaccacctc gtcgatctgg tcccaccaca ccgtctcggg catgaagtcc
     5221 tctccgatcc cgtcgatcaa gtactggtgg atccggtccg ccggcggcat ccggcggtgc
     5281 tcgtggtagt aggcgtaaac actgccgacc gtgtcgaccc cgatcacacg aacgtcgggc
     5341 gattgctcct tgaggtactt cgccacgccg ctgatcgtgc ctcccgtccc gataccagcg
     5401 acccagtggg tgattcggcc gtccgtctgg cgccagatct cgggacccgt gctgcggtag
     5461 tgcgcctcgg gattcgcttg attgtagtac tggtagggca gataggcgcc gcgctcgtcg
     5521 cgaatccgct cggcgacctt gtagtacgat cgcgggtcgt cggccggaac ttcggtgggg
     5581 caggtgatga cctccgcgcc gaatgcctcg agcagcgtga ccttctccgc cgggatcttg
     5641 tcggcggccg tgcagaccag cttgtatccc ttgagcgccg cgaccatcgc cagcccgacc
     5701 ccggtgttgc ccgaagtcgg ctcgacgatc gtaccccccg gtcggagttt gccgctccgt
     5761 tcgccggcct cgaccaacgc cggcccgatg cggtccttga cgctgctccc cgggttgaac
     5821 gactcgatct tggccgcgac gatcgcgtcg ccgggacaga accgcccgag cctgacgagc
     5881 ggcgtattcc ccaacgtgtc gaggatggtc tcgtagatct gcatcgggtc aatcgtggta
     5941 cgcgagctcg gtggccgtcc gggtccacca gccgtcttcg gccggtaggt agaggttgca
     6001 gcgctccatc tcgcccgcgt agacgtggac cgtcaccgag cggtccgccg gcagcgcgtt
     6061 gcgaaggacg tggtgctcga acggcggaat cagacagccg gagtcgccga cgccggcgtg
     6121 caccgaaccg cgtcgctcga agcggcatcg atccccgact cgctccagca ggtcgtactg
     6181 caccacttcg agtcgcccct cgattacgca ctcgacgcac cacatgccgg catgatcgtg
     6241 cagctgggtc tgctggccgg gtccccaggt catcgcgacc acggtgtagc cgcgctcggg
     6301 gcaacggtgg agaagccgcc gcgcgtaggt cgagggattc acgctacgga ccgactcggg
     6361 cagcgcgatc ctgcgctggc tggccagctc gtgaagtccg tcggccaacc ggcgcgtgag
     6421 cgcctcgacg tcgtcctcga ccatcgctcg atcgagcatc tcgatcaacg ggcgatatgc
     6481 tgggaggtcg gtcaagattc ccctcgttgc ggccccaatg tacagaatcc gccgattccg
     6541 cgcgaccctt ttccgggcgc gacgaacgaa gacggaggaa cggccttata ctcgagcgtt
     6601 gccggtgggg gattcgcggt tggatcgttc gtcagtccgc tttctgggaa cgggtacggc
     6661 gttcaatcac gccggccgac ggtcgcagag cttcctggtc cgcgccgccg gccagtcgcc
     6721 gttcctggtc gatgccggtc cgacggtgat ggtcccgctg atgcaggagg gctcggccta
     6781 cgacgagatc gaccggttgt tcgtgacgca tctgcacggc gaccacaccg cgggctggcc
     6841 gttcctgctg ctgcacctcg tcatcctggc gcgtcgacgg cgcccgttcg acgtct
//
LOCUS       DASPGN010000315        18537 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1460595, whole genome shotgun sequence.
ACCESSION   DASPGN010000315 DASPGN010000000
VERSION     DASPGN010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 18537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18537
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460595"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..500
                     /locus_tag="VD788_09140"
     CDS             117..500
                     /locus_tag="VD788_09140"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76471.1"
                     /translation="MVRTALIALSIAAAASAAAEVPRSPAAEAIGLELHADGSMSAEF
                     VDTDLRRVLQAIAELSGFGLVLDPGVEGRVNASFNRVPRKQLLKTILRTHGLSVDGGC
                     SRPGVSAPGNAAGPRAVLIVRPGPS"
     gene            complement(524..1558)
                     /locus_tag="VD788_09145"
     CDS             complement(524..1558)
                     /locus_tag="VD788_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HXV76472.1"
                     /translation="MNTDLSPAEPQLPAPTLLLTGEDVSELLPVADCIRAVEDALRHH
                     ADGRSIPPAVLGIDANRGGFHLKAAGLRLERTWFAVKCNGNFPDNPMRFRLPTIQGLI
                     VLCDGDDGSPLAVIDSAEITSLRTGAATAVAAKHLAREDARDVTICGCGRQGRAQLRA
                     LCCVRPLERVFAFDTDGALARRFAEAMAAETGLRVEPVDDLAFAVRQSDVCVTCTPAT
                     RYLLEAGDVRPGTFVAGVGADSENKWEIDPLLFGRAKVVVDHLEQCATIGDLHHALDR
                     GTIERGQVHAELAELVAGRRAGRTTDEEITLFDSTGIALEDVGAALAVYRRAVERGGA
                     AGVRFGARPR"
     gene            complement(1573..2907)
                     /gene="chrA"
                     /locus_tag="VD788_09150"
     CDS             complement(1573..2907)
                     /gene="chrA"
                     /locus_tag="VD788_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HXV76473.1"
                     /translation="MERPSADRVAGSEGVRFADALRFWLKLGFISFGGPAGQIAIMHR
                     EIVERRRWICETRFLHALNYCILLPGPEAQQLATYVGWLLHGTRGALAAGILFVLPSV
                     FILLALSVAYAAYGEIPAAAGLLAGCKAVVVAIVAEAALRLGRRALRGWVHLLVAGCA
                     FVGIYFLHVPFPVIVLAAATVGLSGARWWPATWTPPGAPPSGAATDATDDGEPIALPR
                     SAPSAVRALRTLAVGILLWLLPLAVLAGWLGRESIFVKQYQFFTGAAFVTFGGAYAVL
                     AYVSQAAVDAYGWITPAAAVDGLALAETTPGPLIMVLQFIGFMTGWNHPDPLDPLTAG
                     TLGALVTTWTTFLPCFLFVLIGAPWIEALRGNRPLHGALSGVTASVIGVIANLALVFG
                     AAVILPDGPGGGVEWFAAGLSALAFLAIYRYRIDALWIVLAGGAAGLVRTWF"
     gene            complement(2870..3907)
                     /locus_tag="VD788_09155"
     CDS             complement(2870..3907)
                     /locus_tag="VD788_09155"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HXV76474.1"
                     /translation="MPQPHRWLLLVHQIPSRPTRLRVRVWRQLQRLGAMAIKNSVYVL
                     PASDKTREDFAWLQQEIESGGGEAVVFEAGPVGGLTDERMIEAFRRDRDAAYARVAAT
                     LGGLSKRLADGKARSSPPRLEQIEQEVDRMQAELHAIDEADFFAAPRRRQAAEELARC
                     RTLLRSAERQRAAPAGPAARPTRDRASYQNRLWVTRPRPHIDRCASAWLIRRFIDRRP
                     RFGFATETGVRRGGIPYDMPGAEFSHHGEDCTFETLMKQFALAGDAALRAIAEIVHDV
                     DLKDGKFGRVEGAGVDAVLRGLAERVRDDGRLLRESVAVFDGLYATLAGMPVAAGRKR
                     GTTKRGSRRRK"
     gene            4113..5024
                     /locus_tag="VD788_09160"
     CDS             4113..5024
                     /locus_tag="VD788_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HXV76475.1"
                     /translation="MTRTRDGERSILTRYLSEIRDRRMLSRDEEVLLALAAQRGDRRA
                     MQELVCANLRFVVRVAHEYRDLGLPFEDLLNEGNVGLLEAARRYDVDRGTKFITFAVW
                     WVRKTILTALTQNANLVRIPEHHLRKLRRIRDAETTLTRVLGRSPEREEVADLLSHGS
                     DRLDETLRVRPRTRSLDEAASRDVEISLGDTLADDSSPTTEEDLLRREARQLVSRALG
                     ELDPQERRVLEYRYGLTDEPAMVLREVGRKLGVSRERVRQIEVKATHRLRRILERRLR
                     GRSPRADAARNLRARSLSPAATEPAPA"
     gene            complement(5158..6000)
                     /locus_tag="VD788_09165"
                     /pseudo
     CDS             complement(5158..6000)
                     /locus_tag="VD788_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            complement(6050..6967)
                     /locus_tag="VD788_09170"
     CDS             complement(6050..6967)
                     /locus_tag="VD788_09170"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXV76476.1"
                     /translation="MNAWGPSTRGLRLLASVLSCAGIVQAQELEPRAYRALPTGLNFL
                     VGAYQYSSGNVVFDGTAPLEDLDIDIHTASLSYLRTFGLAGRSASVSFTAPRVQAAGS
                     AEVAGARVSRSRSGWADARLRLAVNLAGGPALSMQEFAKRRPGRTIGVGLTISVPTGQ
                     YDPQYAVNFGANRWGFKSELGYSSVRRRWILDSAVGVWLFTNNDDGPGGTTVRQDPIW
                     SWQGHISYGFANGMWLAFDLNYFYGGATAADGRETGATQSNSRAGLTWSIPIATRHSL
                     KLGGSTGSYTRAGADFDLATVAYQYQWGR"
     gene            7144..7566
                     /locus_tag="VD788_09175"
     CDS             7144..7566
                     /locus_tag="VD788_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76477.1"
                     /translation="MSRLTLVLLGVLLLLTPAPARADDLTGADRLVCAAMVVVACWET
                     GECEERLPSELNVPQFIEIDLKQKRLSTTEASGEDRSTPVTTLLRQDGAIVLQGHEAG
                     RAFSFVISEATGKASVAVARDGLGVSVFGACTPALSSR"
     gene            7563..7760
                     /locus_tag="VD788_09180"
     CDS             7563..7760
                     /locus_tag="VD788_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76478.1"
                     /translation="MNRRLATLGLGVLVAATVTLAHGSATAPPVEQQTPEGQQATQQP
                     VGSFKKAFSVCLEAKDYLVKF"
     gene            7777..8034
                     /locus_tag="VD788_09185"
     CDS             7777..8034
                     /locus_tag="VD788_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76479.1"
                     /translation="MAARPRPVVERVQTGVRLEKRLLKVLKGLAEYHELSLGDLLEGI
                     VLHAFENKRPFSSESLAVIADLKRVYGLDLRARDSHRLTER"
     gene            8100..8474
                     /locus_tag="VD788_09190"
     CDS             8100..8474
                     /locus_tag="VD788_09190"
                     /inference="COORDINATES: protein motif:HMM:NF042861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HXV76480.1"
                     /translation="MGRPIANTEARRAGILAQAVTRAARALGLSQKAVAAALGVSEAT
                     VSRLARGRAIDPETKEGELALLVIRLFRSLDSLVGGDAAKTRDWMRAHNLHLRGVPAE
                     LIASVSGLVHVVEYLDALRGKS"
     gene            8434..9198
                     /locus_tag="VD788_09195"
     CDS             8434..9198
                     /locus_tag="VD788_09195"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HXV76481.1"
                     /translation="MSSSIWTRCGEKANLRRLGGRVWRVVEAQHVTATRKLVDTLEQQ
                     ETLERLIDGSKPAAPTRAGARPLHYLLFTPFRYPPLGHGSRFGSRADPSLWYGSSRPR
                     TAFAECAYYRLLFLEGTTADLGSVAVDLSLFDVTIATRRGVDLTRPPFDAHRAAIASP
                     TDYAGSRSLGRAMREAGVEAFRYPSARDARGGVNVALFTPAPFAGGNPGAPETWHSVT
                     SRRRVEFVKKDVFERRSFVFAREEFEVAGRLPAPAL"
     gene            9218..10942
                     /locus_tag="VD788_09200"
     CDS             9218..10942
                     /locus_tag="VD788_09200"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HXV76482.1"
                     /translation="MRRTRAAGRTITREVIVRFVAWLLPIAALFVVYPVVLYQRELDA
                     ELRFHEQENLRVIDVGAEVVTRDLQSAVADLSYLAGQSVVRDYLAHGAVARARLERDF
                     LSFCRHKGIYDQIRFIDAEGAERVRVNYDGGTPRVVAEAELQSMAAQEYFTAAIALDP
                     GQVYVSPFEINVEHGEIERPLKPVIRFATPIADDGRVAGVLVLNYLGRRVLDKLREVS
                     AGYRGEISLIDGDGFYLMHPHSPEAEWGFQLGPGPNFATDHPAAWKAVATRAAGSVVS
                     GEGLFTFRRIGPAGSPSRGLVLVAHRTASQLGERARGLLAHVLSAYSLAGPVLLVLIG
                     YLAFAGAARRRQQEQIAESAARLRVLSTQLLTAQERERAGLSRDLHDDLGQVVTAVRL
                     DLERAARETDPRDKDRLIAEALAASKTLMERVRAISTRLRPRILDDLGLKEATRSFLA
                     DCQRHLGVAVDADLRFERAEIPPEISENVYRILQEAVTNVARHSGVERCSVELHVVED
                     GLRLSVTDGGTGFDAEAALARGLGLLGMRERARLLGGTFRLRSAPGDGTRIDVLIPLP
                     PAGEPRRP"
     gene            10939..11589
                     /locus_tag="VD788_09205"
     CDS             10939..11589
                     /locus_tag="VD788_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV76483.1"
                     /translation="MNTAGKIRVVLADDHTMVREALAGILDQHDEIEVVGQASDGSEA
                     VALVERLGPQVLVLDYSMPGNDAPKVIEALARAGLEVKILILTVHENIHYAVRVLESG
                     ALGYVVKTAAAQELVEAIRAVERGEVYISRAVSQRVLQQLRRPRTDRLGVGALSQREF
                     ELLRVLGAGLSLKECAEALNISTSTASTYRARLMQKLGLRTTAEIIRFALEHDVIG"
     gene            11722..14538
                     /locus_tag="VD788_09210"
     CDS             11722..14538
                     /locus_tag="VD788_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXV76484.1"
                     /translation="MAGTVEGKQRYVPAVGPGLKKLLAVVFALFALLVVNSVYLVSIT
                     IAGTHYQGWFYLMMFALHLLLGLALVLPVVVFGAQHIRNARNRRNKRAIRVGYALFSV
                     AISVLVTGIVLMRVDLFGARLEINDPLARSAAYWLHVVLPLAAIWLFVLHRLAGRRIR
                     WKVGLAWASVAALFALGMLALHSQDPRAWNVVGPKSGEQYFFPSLSRTSDGNFIPAEV
                     LDNDHYCQECHADIHDQWSHSVHRFSSFNNPPYLFSVKNTRRAMMARDGTVQGSRFCA
                     GCHDPVPFFSGQFDDPKFDDPEFDLASDETAQAGITCTVCHSISHVNSVRGNADFTID
                     EPVHYPFAFSENPTLRWVNRQLVKAKPEFHKATFLKPLHRTTEFCGTCHKVHLPPELN
                     AYKWLRGQNHYDAFWLSGVSGHGIASFYYPPKAEPNCNNCHMPLAEVSERPNFAAKVR
                     DESGALKTFGHQFPSANTAIPHLVRDQIADADRAIDEHRRFNEGVMRVDIFGVKSDGA
                     IDGELTAPLRPELPALAPGETYLIEIVIRTVKIGHLFTQGTVDSNEVWMDVRASNAER
                     VIGHSGWRDARDNSVDPESHFVNAFVIDRQGDRINRRNAEDIFIALYNNQIPPGAADV
                     VHYRLRVPRDARGSIAIEAKLLYRKFDTEYMRLVTEDASYYNDLPIMTLAEDRVVFPV
                     GGSRPIEQTEPAIERWQRWNDYGIALLDKGQLGELRQAAQAFAEVEKLGRADGPLNLA
                     RVYIKEGLVQQQAPAALARAAANDPPANEWSLLWFGAVIAEANGDYDLAIANLERIVR
                     GGFEQAQGRDFEFHKDYRVQNQLGNALYQRGLLEQGETRKSYLERARDRFLEALSYDP
                     ENLTAHWGLQRVYGDLGTADKAREHAELHAKYKPDDNARDYAAAQARLKYPAANRAAE
                     AVVIYDLDAPVEVDDALEVAKVEN"
     gene            14528..16384
                     /locus_tag="VD788_09215"
     CDS             14528..16384
                     /locus_tag="VD788_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HXV76485.1"
                     /translation="MKTDTEGHDRTTPEEEWVPEDDAVIGKAFRWSMLVGAILVLVVL
                     ATLWSLRERPEVETFVDSGPVAVPQALRQETAGMPTVAFTDITERAGIDFVHNSGAYG
                     AKLLPETMGGGVAFLDYDNDGDEDLLFVNSADWPDHRRGGSAPMTLYRNDGRGRFDDV
                     TRATGLDVSFYGQGAAVGDYDNDGDVDLFLTALGANHLFRNVDGRFHDVTARAGVAGA
                     DDDWSTSAGFFDYDIDGDLDLFVCNYVRWTREIDMRLAFTLNGTDRAYGPPKLYQGVH
                     SSLYRNEGDGTFSDVSFEMGIQVNNPATDKPMGKALAVTFADVDHDGYPDVFVANDTV
                     QNFLFRNNGGRSFSEIGAISGVGFDNQGTATGAMGIDAADIAGDGRLGVAIANFSNET
                     TSFYVQQTDPWQFVDMANAVGVGPPSRLKLSFGLFFFDYDLDGRLDMLQANGHLEDEI
                     NEIQPSQHYRQTPQLFWNCGSDGGTCFAAVADEQLGALATPIVGRGAAFADIDADGDL
                     DIVLAQTGGPALLLRNDQQLGRHWLRVRLAGNRGNRDAIGAGLELRAGGRTQKRVVMP
                     TRSYLSQVERTVTFGLGENERVDSLVIRWPGGRTQQVTVGQVDTTLVVNEGS"
     gene            complement(16412..17776)
                     /locus_tag="VD788_09220"
     CDS             complement(16412..17776)
                     /locus_tag="VD788_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HXV76486.1"
                     /translation="MDYQTTDPTTGKTLECYPTATGAECRGAIERAAAAFAAWRATPV
                     EARAERLRRCAESLDRQAPELAELMAVEMGKPLVEGEAEAAKCAWGCRFFAEQAASFL
                     EPDAKDSDGSQAYVRYDPLGPLLAIMPWNFPFWQFFRFAAPALMAGNVVLLKHAPNTP
                     GCARRIERLLGESGLPDGVVQNLFLTDEQAAGVIADPRVRGVTLTGSTRAGRQVAAAA
                     GRQLKAMVMELGGSDPFIVFGDADLERAADTAVASRCLNNGQSCIAAKRFLVEASVFD
                     RFRDAVVERMRSRKIGDPRDRSVELGPLARRDLRDTLADQVDRTVAAGARVLCGGEPP
                     ARDGFFYPATVLVDIPAGSPAAREELFGPVASLFPFDDERQAVALANDSEYGLGASLW
                     TRERERVDRLIPRLDSGSVFVNGLVKSDPRLPFGGVKQSGFGRELSREGMLEFVNRKT
                     VWIA"
     gene            complement(17833..>18537)
                     /locus_tag="VD788_09225"
     CDS             complement(17833..>18537)
                     /locus_tag="VD788_09225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV76487.1"
                     /translation="VAVKAVSGVLAAGDALDRFEREAKLLARLNHPNIATVHDLIVVD
                     RKPYLILELVRGTTLSELMRGGPLPPGEVRRIALELCDGLAEAHAHGIVHRDLKPANV
                     MITPAGRVKVLDFGLAKTVERDRDGSGAFSTAASTMLGTPGYMSPEQIRGEPVDARAD
                     LWALGCILFEMLTGDRAFAGSCASDTIAAVLRDEAELGRLPADTPVELSESIRRCLDK
                     SPERRPPSVDELRRRL"
BASE COUNT         2956 a         6444 c         6447 g         2690 t
ORIGIN      
        1 gtacctcggt ctggcgcccg ggttgatcct cgagacgatc gccggtctgg cggcgtcgtt
       61 ggtctgatcc gcgccgcgga tagcgcgggc gacgcagcgg tcgtagactc tccggcatgg
      121 tgcgaaccgc gttgatcgcg ctatcgatcg cggcggcggc gtcggccgcc gccgaggttc
      181 cgcgctcgcc ggcggcggag gcgatcgggc tcgagctgca cgccgacggg agcatgtcgg
      241 ccgagttcgt cgataccgac ctgcgccggg tgctgcaggc gatcgccgag ctgagcggct
      301 tcgggctggt gctcgatccg ggcgtcgagg ggcgagtcaa cgcgtcgttc aaccgggtac
      361 cgcgcaagca gctgctcaag acgatcctgc gcacgcacgg cttgtcggtc gacggcgggt
      421 gcagtcgtcc cggtgtttcc gcgccgggga acgcggccgg cccgcgggcc gtgctgatcg
      481 tgcgaccggg cccgtcctag cccggccgcc ggccgccggg cgtctatcgg ggccgtgcac
      541 cgaaccggac gccggccgcg ccaccccgct cgacggcccg ccggtagacc gcgagcgccg
      601 cgccgacgtc ctcgagcgcg ataccggtgc tgtcgaacag cgtgatctcc tcgtcggtcg
      661 tgcgccccgc tcggcgtccg gcgacgagct cggccagctc ggcgtgcacc tggcctcgtt
      721 cgatcgtgcc gcgatcgagg gcgtggtgca gatcgccgat cgtggcgcat tgctcgaggt
      781 ggtcgacgac gaccttggcc cggccgaaca gcagcgggtc gatctcccac ttgttctcgc
      841 tgtcggcacc gacgccggcg acgaacgtac ccggtcggac gtccccggct tccagcaggt
      901 agcgtgtcgc gggagtacag gtgacgcaga cgtcactctg ccggacggcg aacgccagat
      961 cgtcgaccgg ctcgacccgc aggccggtct cggcggccat cgcctcggcg aagcggcggg
     1021 cgagcgcacc atcggtatcg aacgcgaaga cgcgctcgag cggccggacg cagcacagcg
     1081 cgcgaagctg ggcgcggccc tggcgcccgc aaccgcagat ggtgacgtcg cgggcgtcct
     1141 cccgggccaa gtgcttcgcc gccaccgccg tcgccgcgcc ggtgcgcagc gaggtgatct
     1201 ccgccgagtc gatcaccgcg agcggagagc cgtcgtcgcc gtcgcacagc acgatcagcc
     1261 cttggatcgt cggcaggcga aaacgcatcg gattgtcggg gaagttgccg ttgcacttca
     1321 ccgcgaacca cgtgcgctcg agcctgagcc ccgcggcctt caggtggaag ccgcctcggt
     1381 tcgcgtcgat gccgaggacc gcaggcggga tcgaccgccc gtccgcgtgg tgccgcaacg
     1441 cgtcttccac ggctcggatg cagtccgcga ccggaagcag ctccgagacg tcctcgcccg
     1501 tcaggagcag tgtcggtgcc gggagctgcg gctcggccgg cgacaggtcg gtgttcatcg
     1561 ctttacacct cgtcagaacc aagtgcgcac gagaccggcg gcgccacccg cgagcacgat
     1621 ccagagcgcg tcgatccgat agcgatagat ggcgaggaac gcgagggccg aaaggccggc
     1681 ggcgaaccac tcgacgcccc cgcccggacc gtccgggagg atcaccgccg ctccgaagac
     1741 gagagcgagg ttggcgatca cgccgatgac cgaggcggtg accccggaca gcgcgccgtg
     1801 gagcggccgg ttgccgcgca gcgcctcgat ccacggggcg ccgatcagca cgaacaggaa
     1861 gcaggggagg aaggtggtcc atgtcgtcac cagcgctccg agcgtgccgg cggtcagcgg
     1921 gtcgagcggg tccgggtggt tccatcccgt catgaagccg atgaattgga ggaccatgat
     1981 cagcggaccg ggcgtggtct cggccagcgc cagaccgtcg acggccgcgg ccggtgtgat
     2041 ccaaccgtag gcatcgaccg cggcctggct gacatacgcc agcacggcat aggcgccgcc
     2101 gaaggtgacg aacgccgccc cggtgaaaaa ctggtactgc ttgacgaaga tgctctcccg
     2161 gccgagccag ccggcgagca ccgccagtgg aagcagccac agcaagatcc ccacggcgag
     2221 cgtgcgcagc gcgcggacgg cggagggtgc gctgcggggc aaagcgatcg gctcgccgtc
     2281 gtccgtcgcg tccgtcgctg ccccggacgg cggcgccccg ggcggggtcc acgtcgcggg
     2341 ccaccatctc gcacccgaca gcccgaccgt ggccgccgcc agcacgatca ccgggaacgg
     2401 aacgtggagg aagtagatgc cgacgaaggc acagccggca acgagcagat ggacccatcc
     2461 gcgcagcgca cgccgtccga gacgtagcgc cgcctcggcc acgatcgcca ccaccaccgc
     2521 cttgcagccc gcgagcagcc cggccgccgc ggggatctcg ccgtaggcgg cgtaggcgac
     2581 ggacagcgcg agcaggatga agaccgacgg caacacgaac aggatgcccg cggcgagcgc
     2641 cccgcgagtg ccgtgcagca gccagccgac gtaggtcgcg agctgctggg cctccgggcc
     2701 gggcaggagg atgcagtagt tgagcgcgtg gaggaagcgg gtctcgcaga tccagcgccg
     2761 ccgctcgacg atctcccggt gcatgatcgc gatctgaccc gccgggccgc cgaacgagat
     2821 gaagccgagc ttgagccaga atcggagggc gtccgcgaag cgtacgccct cacttccggc
     2881 gacgcgatcc gcgcttggtc gttccacgct ttctcccggc agcgaccggc atcccggcca
     2941 acgtggcgta gaggccgtcg aagaccgcca ccgactcgcg aagcagccgt ccgtcgtcac
     3001 ggacccgctc cgccagcccg cgcaggaccg cgtcgacacc ggccccctcg acgcggccga
     3061 acttgccgtc cttcaagtcg acgtcgtgca cgatctcggc gatcgcgcga agggccgcgt
     3121 cgcccgccaa ggcgaactgc ttcatcagcg tctcgaacgt gcagtcttct ccatggtggc
     3181 tgaactccgc gccgggcatg tcgtagggga tgccgccgcg gcgcacaccg gtctccgtgg
     3241 cgaacccgaa ccggggtcgg cggtcgatga accggcggat cagccaggcc gaagcgcagc
     3301 ggtcgatgtg cggtcgcggg cgggtgaccc acaggcggtt ttggtagctc gcccggtcgc
     3361 gggtcggccg agcggcgggc ccggcgggcg cggcccgctg tcgctcggcc gatcggagca
     3421 gggtccggca gcgggccaat tcctcggcgg cctgccggcg cctcggtgcg gcgaaaaagt
     3481 cggcttcgtc aatcgcgtgt agctcggcct gcatgcgatc cacctcctgt tcgatctgtt
     3541 ccagacgcgg tggggaggag cgggccttcc cgtcggccag ccgcttcgac agcccgccaa
     3601 gggtggcggc gacccgcgcg taggccgcgt cgcggtcccg ccggaaggcc tcgatcatcc
     3661 gctcgtcggt cagcccgccg acgggacccg cctcgaaaac gaccgcctcg cccccacccg
     3721 actcgatctc ctgctggagc caggcgaagt cttctcgcgt cttgtccgag gcgggcagga
     3781 cgtagaccga gttcttgatc gccatcgctc ccagtcgctg gagctgacgc cagactcgca
     3841 cgcgcagccg cgtcggccgg gacgggatct ggtggaccaa cagcagccag cggtgtggtt
     3901 ggggcatcgt cgaaatgtaa cagatgttac tctacgatgt caagtgttac aaggccagat
     3961 cgacggcgga ggcgggcctt cggccggggc gcggaacccg cggacgagac ctcccgcgtt
     4021 gttcaccaaa cgttcacatc gttgcccgca gtccgtcgtt cgcggcaggc ccaatgccga
     4081 tcccggcatt cgggacgacg acggagcgac gcatgacgag aacgcgcgac ggggaacgat
     4141 cgatcctcac acgctacctg tccgagattc gcgaccgccg aatgctgagc cgggacgagg
     4201 aggtgcttct ggcgctcgcc gcgcagcgcg gagatcgccg ggcgatgcag gagctggtct
     4261 gcgcgaacct gcgtttcgtc gtccgggtcg cccacgagta ccgcgacctg ggtttgccgt
     4321 tcgaggacct gctgaacgag ggcaacgtcg gcctgctcga agcggcccgg cggtacgacg
     4381 tcgaccgtgg aaccaagttc atcaccttcg ccgtctggtg ggttcgcaag acgatcctga
     4441 ccgcgctgac gcagaacgcc aatctggtcc ggattccgga gcaccacttg cgcaagctgc
     4501 ggaggatccg cgatgcggaa acgacgctga cccgcgtgct cggccggtcg ccggagcggg
     4561 aggaggtggc cgacctgttg tcgcacggct ccgaccggct cgacgaaacg ctgcgcgtgc
     4621 gccccaggac ccgcagtctc gacgaggcgg cctcccgtga cgtggagatc tcgttaggcg
     4681 ataccttggc cgacgactcg tcgcccacga ccgaggagga tctcttgcga cgcgaagcgc
     4741 ggcaactcgt ctcgcgcgcc ctgggtgagc tcgacccgca ggagcgacgc gtgctcgagt
     4801 accgctacgg gctgacggac gagccggcga tggtgctgcg cgaggtcggg cgcaagctgg
     4861 gcgtcagccg cgagcgcgtg cgtcagatcg aggtcaaggc gacccaccgc ctgcgccgga
     4921 ttctcgagcg gcgattgcgc ggtcgatcgc cgcgggcaga cgccgcccgg aacctgcgcg
     4981 cgcgctctct ctcgcctgcc gcgaccgagc cggcacccgc gtagctcgcc gacggggcgc
     5041 gagcgcggag agcaacgcgg cggcgccgcg cagcaggcgt accgcgttgc cggtcgccat
     5101 cggcccgacg cagctccaca gcacgacctc ctccgcgccc atcgcgcgca gccgcgcgac
     5161 gacggccgcc gcttcgcccg tgctgccgaa cgcggcggtg ccgcggagcc gcagccggtc
     5221 gtacaacggg acgtcgtcct cgcgtggaac cccctgagct tcggcgaacc gctcgagcgg
     5281 ctcgagcatc aggcgtcgag ccgaccgggc cgacggcgcg acggccgcca tcagcacgac
     5341 ggcattcggc acggctcgtc gatcgagccc cgcttcgcgc atgccgcggc ggtgctcggc
     5401 gagacgcgac gcgatcggat cgaggctgcc gagcgtcggc gagacgagcg tcaacaatcc
     5461 caacccgtcg cggcctgcga ggtaggccga ctcgccgcgg ttggtcgcga cgtagagcgg
     5521 cggcagcgga cgctggagcg gacgaagacc gagtcgcagc ccgcccgcgc cgccgccatc
     5581 gtcgccccac agccggcgga gctcggcgag ccggtcgacg aagaccgtcg gtctcgtttc
     5641 ggggtcgagc ccgaaggccc gaaactcggc ctcgaccgct cccgacccga cgcccaacac
     5701 cagccggccg ccggacagcc ggtcgaccag cgcgtagtct tcggcggtga acaccgcctg
     5761 gcggaacggc aggacggcga cggccggtcc caggcgaatc cgccgcgtgc gcgtcgcgac
     5821 ggccgcgagg aagacggccg gattcggcat gccccagtcg tcgccgaagt ggtgctcggc
     5881 gacccacagc gtatcgaggc ccgactcgtc ggccgcgacg gcgaggtcga gcccccgccg
     5941 aacgacgtcg atctccgcgc cgcgccctcg atcgaacaac aggaacgtcg acagcttcat
     6001 cggggaatgg tacacgagta ccttggtaca cgcaacctcc ctcccggggt cagcgacccc
     6061 actggtactg gtaggcgacc gtggcgaggt cgaaatccgc cccggcacgt gtgtacgaac
     6121 cggtcgagcc gccgagcttg agcgagtgtc gggtcgcgat cgggatcgac cacgtcaacc
     6181 ccgccctcga gttggactgc gtcgcgccgg tctcgcgacc gtcggccgcg gtcgcaccgc
     6241 cgtagaagta gttgagatcg aacgcgagcc acatcccatt ggcgaacccg tagctgatgt
     6301 gcccctgcca gctccagatc gggtcctggc ggaccgtggt gccgcccggg ccgtcgtcgt
     6361 tgttcgtgaa cagccacacg ccgactgcgc tgtcgaggat ccaccgccgg cgcacgctcg
     6421 agtagcccag ctccgacttg aagccccagc ggttggcacc gaagttgacg gcgtactgcg
     6481 gatcgtactg cccggtcggg accgagatcg tcaagccgac gccgatcgtc cgccccggtc
     6541 tgcgcttcgc gaactcctgc atcgacagcg ccggaccacc ggccagattg accgcaagac
     6601 gcaggcgggc gtcggcccac ccgctgcggc tgcgcgacac gcgtgccccg gcgacctcgg
     6661 ccgagcccgc ggcctggacg cgcggggcgg tgaagctgac cgacgccgac ctaccggcga
     6721 gcccgaaggt gcgcaggtag gacaggctcg cggtatggat gtcgatgtcg agatcctcga
     6781 gcggcgccgt gccgtcgaag acgacgttgc cggaggagta ctggtaggcg ccgacgagga
     6841 agttgagacc ggtcggcagc gcgcgatacg cccgcggctc gagctcctgc gcctggacga
     6901 tcccggcgca cgacagcacc gacgccagca gcctgagccc ccgagtcgac ggaccccacg
     6961 cgttcaagcg gcacaagttc gcccccgaga gctcggctca tgatagcccc gacgtcgacc
     7021 cgaatggaac ggtgcccggt cgtttcggga gaggaaatgt ccggcgagat gagcgagaat
     7081 gacgccgact cggtacgacc gagcgcctcg cgatcgtgcg aggaccgagg aggtggaatg
     7141 gtcatgtccc gactgacgct tgtgctactc ggcgtgctcc tgctgctgac ccccgcaccg
     7201 gcccgggccg acgatctgac gggagccgac cggctggtct gcgcggcgat ggtcgtcgtc
     7261 gcgtgctggg agaccggcga gtgcgaggaa cgtctgccgt ccgagctcaa tgtgccgcag
     7321 ttcatcgaga tcgacctgaa acagaagcgg ttgagcacga ccgaggccag cggcgaggac
     7381 cgctcgaccc ccgtcacgac cctgctgcgc caggacggcg cgatcgtgtt gcagggccac
     7441 gaggcggggc gggccttcag cttcgtcatc tcggaggcga cgggcaaggc gtccgtcgcg
     7501 gtcgcgcggg acgggctcgg cgtttcggtg ttcggtgcct gcacaccggc gctgtcctcg
     7561 cgatgaaccg acgccttgca acactcggcc tcggcgtgct cgtcgcggcc accgtcaccc
     7621 tggcccacgg gtcggcgacc gcccccccgg tcgagcagca gacccccgag gggcagcagg
     7681 cgacacagca gccggtcggc agtttcaaga aagcgttcag cgtctgtctg gaagccaagg
     7741 attacctggt caagttctag acgcggagga gaaccgatgg ctgccagacc gcgccccgtc
     7801 gtcgaacgag tacagacggg cgtgcggctc gagaagcgac tgctgaaggt gctcaagggg
     7861 ttggccgagt accacgagct ttcgctgggg gacctgctcg aggggatcgt cctgcacgcc
     7921 ttcgagaaca agcggccgtt ctcgtccgag tcgctcgcgg tgatcgccga cctgaagcgg
     7981 gtctacgggc tcgacctgcg tgcccgcgac agccaccggc tcaccgaacg ctgagggacg
     8041 cgggtcgtcg ttgcacggga aagaatcgca tactattatt tcctgtgaga aaccaccgaa
     8101 tgggacgacc gatcgcgaac acggaggcca ggcgggcggg gatcctggcc caggccgtca
     8161 cccgggccgc gcgggcgctc ggcctcagcc agaaggcggt cgccgccgcc ctcggcgtca
     8221 gcgaggcgac cgtctcgcgc ctggcgcgcg gtcgcgcgat cgaccccgaa acgaaggagg
     8281 gtgagctggc gctgctcgtc atccgcctgt tccgttcgct cgactcgctg gtcggcggcg
     8341 acgcggcgaa gacgcgcgac tggatgcgtg cgcacaacct ccacctgcga ggggttcccg
     8401 ccgagttgat cgccagcgtc agcggactgg tccatgtcgt cgagtatctg gacgcgctgc
     8461 ggggaaaaag ctaacctccg ccggctcggc ggccgggtct ggcgggtcgt cgaggcccag
     8521 cacgtcaccg ccacgcgcaa gctggtcgac acgctcgagc agcaggagac cctcgagcgg
     8581 ttgatcgacg gctcgaagcc cgcggccccg acccgcgccg gagcacgacc gctgcactac
     8641 ctgctgttca caccgtttcg ctaccctcca ctcggtcacg gttcgcgatt cggcagccgc
     8701 gcggaccctt cgctgtggta cggctcgtcc cgtccgcgga cggcgttcgc cgagtgcgcc
     8761 tactaccggc tgctgttcct cgaaggcacg acggccgatc tcggctcggt cgccgtcgat
     8821 ttgtcgctgt tcgacgtgac gatcgccacg cgacgtgggg tcgatctcac tcgcccgccg
     8881 ttcgacgcgc atcgcgcggc gatcgcttcg ccgacggact acgccggcag ccgcagcctg
     8941 ggacgcgcga tgcgcgaggc gggtgtcgag gcctttcgct acccgtcggc ccgcgatgcg
     9001 cggggcggcg tcaacgtcgc gctgttcacg cccgcgccgt tcgccggcgg caacccgggc
     9061 gcgcccgaga cctggcattc ggtgaccagc cgccggcggg tcgagttcgt gaagaaagac
     9121 gtcttcgagc ggcgctcgtt cgtcttcgcc cgcgaagaat tcgaagtggc cggtcggttg
     9181 ccggcgccgg cgctgtagcg ccggagacga tgtgccgatg cggcgaacga gagcggccgg
     9241 tcggacgatc acgcgcgagg tcatcgtgcg tttcgtggcc tggctgctgc cgatcgccgc
     9301 gctgttcgtc gtctatccgg tcgtgctcta ccagcgcgag ctggatgccg agctgcggtt
     9361 ccacgagcag gagaacctgc gggtaatcga cgtcggggcc gaggtcgtca cgcgcgacct
     9421 gcaatcggcc gtcgccgacc tgagctacct cgccgggcaa agcgtcgttc gcgactacct
     9481 ggcgcacggc gccgtggcca gagcgcggct cgagcgcgac ttcctgtcgt tctgtcggca
     9541 caagggcatc tacgaccaga tccgcttcat cgacgcggag ggcgccgagc gggtgcgggt
     9601 gaactacgac ggcggaacgc cgcgggtcgt cgccgaagcg gagctacagt cgatggcggc
     9661 acaggagtac ttcaccgctg ccatcgcgct cgaccccggg caggtgtacg tctcgccgtt
     9721 cgagatcaac gtcgagcacg gcgagatcga acggccgctg aagccggtga tccgcttcgc
     9781 cacaccgatc gccgacgacg gccgggtcgc gggcgtgctg gtgctgaact acctgggccg
     9841 tcgcgtgctc gacaagctgc gggaggtgtc ggcgggctat cgcggcgaga tctcgctgat
     9901 cgacggcgac ggcttctacc tgatgcaccc gcactcgccg gaggcggaat ggggcttcca
     9961 actcggtccc ggtccgaact tcgcgaccga ccaccccgcg gcgtggaagg ccgtcgcgac
    10021 ccgggcggcg ggcagcgtcg tgagcggcga aggcctgttc accttccgcc gcatcggccc
    10081 ggccggctcg ccgagccgcg ggctggtgct ggtcgcccac cgcacggcgt cccagctcgg
    10141 cgagcgcgcc cgcggcttgt tggcacacgt gctgtcggcc tactcgctcg ccggcccggt
    10201 gctgttggtg ctgatcggct atctcgcctt cgccggcgcc gctcgacgac ggcagcagga
    10261 gcagatcgcc gagtcggcgg cccggctgcg cgtgctgtcg acgcaactgc tgaccgcgca
    10321 ggagcgcgag cgggccggtc tctcgcgcga cctgcacgac gacctcggcc aggtcgtgac
    10381 cgccgtgagg ctcgacctcg agcgggccgc tcgcgagacc gacccgcgcg acaaggaccg
    10441 cttgatcgcc gaggcgctcg ccgcctcgaa gacgctgatg gagcgggtgc gcgcgatctc
    10501 gacccgcctg cgcccgcgga ttctcgacga tctcgggctg aaggaggcca cccgcagctt
    10561 cctcgccgac tgccagcggc acctcggcgt ggcggtcgac gccgacctgc ggttcgagcg
    10621 cgcggaaatc ccgcccgaga tcagcgagaa tgtctaccgc atcctgcagg aggcggtgac
    10681 caacgtggcc cgacattccg gcgtcgagcg gtgctcggtc gagttgcacg tcgtcgagga
    10741 cggcctgcgc ctgtcggtca ccgacggcgg cacgggcttc gacgcggagg ccgccctggc
    10801 gcgcgggctc ggcttgctcg gcatgcgcga gcgcgcgcgg ctgctcggcg ggaccttccg
    10861 gctgcgatcc gctcccggtg acgggacccg catcgacgtc ctcattccgc ttccgccggc
    10921 cggcgagcct cgccgcccgt gaacacggcg ggcaagatcc gtgtggtgct ggccgacgac
    10981 cacacgatgg tgcgcgaggc gctggccggg atcctcgacc agcacgacga gatcgaggtg
    11041 gtcggacagg cgagcgacgg cagcgaagcg gttgcgctcg tcgagcggct gggcccgcag
    11101 gtgctggtgc tcgactacag catgccgggc aacgacgcgc ccaaggtgat cgaggcgctc
    11161 gcgcgggccg ggctcgaggt gaagatcctg atcctgaccg tgcacgagaa catccactac
    11221 gccgtgcgcg tgctcgagag cggggcgctc ggctacgtgg tcaagaccgc agcggcgcaa
    11281 gagctggtcg aagcgatccg cgccgtcgag cgcggcgagg tctacatctc gcgcgcggtc
    11341 tcgcagcgcg tgctgcagca gttgcgccgg ccgcgcaccg accggttggg cgtcggcgcc
    11401 ctgtcccaac gcgagttcga gctgctccgc gtgctcggag ccggtctgag cctgaaggaa
    11461 tgtgccgaag cgctcaacat cagcaccagc accgcatcga cctaccgggc gcggctgatg
    11521 cagaagctcg gattgcgcac gaccgccgag atcatccgct tcgcgctcga gcacgacgtg
    11581 atcggctgag ccggaccagc cgggctgtag gatttcagct acaacgatat cgcgttctcg
    11641 gcgactggac tcgccggagc cggcagcgca tctttacatt tcgcacaata acgatcggat
    11701 tcgtccattc tcgggaagac catggcgggc actgtcgagg gcaagcaacg ctacgtaccg
    11761 gccgtcgggc ccggtctgaa gaagctgttg gccgtcgtct tcgccctgtt cgcgttgctc
    11821 gtggtcaact cggtctatct ggtcagcatc acgatcgccg gcacgcacta ccagggctgg
    11881 ttctatctga tgatgttcgc cctgcacctg ctgctcggac tggcgctggt gctgccggtc
    11941 gtcgtcttcg gcgcgcagca catacgcaac gcgcggaacc gacgcaacaa gcgcgcgatc
    12001 cgcgtgggct acgcgctgtt ctccgtcgcg atcagcgtgc tcgtgaccgg catcgtcctg
    12061 atgcgtgtcg acctgttcgg cgcacgtctc gagatcaacg atccgctggc gcgctcggcg
    12121 gcctactggc tgcacgtcgt gctgccgctg gcggcgatct ggctgttcgt gctgcaccgg
    12181 ctggccgggc gtcggatccg gtggaaggtc ggcctggcct gggcctccgt cgccgcgctg
    12241 ttcgcgctgg ggatgctggc gctccacagc caggacccgc gcgcctggaa tgtggtcggg
    12301 cccaagtcgg gcgagcagta cttcttcccg tcgctgtcgc gaacgtcgga cggcaacttc
    12361 attccggccg aggtgctgga caacgaccac tactgccagg agtgtcacgc cgacatccac
    12421 gaccaatggt cccacagcgt ccatcgcttc agctcgttca acaacccgcc ctacctgttc
    12481 agcgtgaaga acacgcggcg agcgatgatg gcccgcgacg gtaccgtgca aggctcccgc
    12541 ttctgcgccg gctgtcacga cccggtgccg tttttctccg gccagttcga cgaccccaag
    12601 ttcgacgacc cggagttcga tctggccagc gacgagaccg cgcaggccgg catcacctgc
    12661 accgtctgcc acagcatctc gcacgtgaac agcgtccgtg gcaatgccga cttcaccatc
    12721 gacgagcccg tgcactatcc gttcgcattc agcgagaacc cgacgctgcg ctgggtcaac
    12781 cgccagctgg tcaaggccaa acccgagttc cacaaggcga cgttcctcaa accgctgcac
    12841 cgcacgacgg agttctgcgg gacgtgccac aaggtgcacc tgccgccgga gctgaacgcc
    12901 tacaagtggc tgcgcggtca gaaccactac gacgccttct ggctcagcgg cgtgtcgggt
    12961 cacggcatcg ccagcttcta ctatccgccg aaggccgagc cgaactgcaa caactgtcac
    13021 atgccgctcg cggaagtgtc ggagcggccg aacttcgcgg ccaaggttcg cgacgagtcc
    13081 ggtgcgctca agaccttcgg ccatcagttc ccgtcggcca acacggcgat cccacatctg
    13141 gtccgcgatc agatcgccga cgccgatcgg gcgatcgacg agcaccgccg gttcaacgaa
    13201 ggcgtgatgc gagtcgacat cttcggtgtc aagagcgacg gcgcgatcga cggcgagctg
    13261 acggcgccgc tgcggcccga gctgccggca ctggcgccgg gcgagaccta cctgatcgag
    13321 atcgtgatcc ggacggtcaa gataggccac ctgttcaccc agggcaccgt cgattcgaac
    13381 gaggtctgga tggacgtccg tgcgtcgaac gccgagcggg tgatcggcca cagcggctgg
    13441 cgcgacgcgc gcgacaattc ggtcgacccg gagtcgcact tcgtcaacgc gttcgtcatc
    13501 gatcgtcagg gcgatcggat caaccgccgc aacgcggaag acatcttcat cgcgttgtac
    13561 aacaaccaga ttccacccgg cgcggccgac gtggtgcact accgcctgcg cgtgccgcgc
    13621 gacgcgcgcg gctcgatcgc gatcgaggcc aagctgctct accgcaagtt cgacaccgag
    13681 tacatgcggc tggtgaccga ggacgcgagc tactacaacg acctgccgat catgacgctc
    13741 gccgaagacc gcgtcgtctt cccggtgggc gggtcgcggc cgatcgagca gaccgagccg
    13801 gcgatcgaac ggtggcagcg ctggaacgac tacgggatcg cgctgttgga caagggccag
    13861 ctcggcgagc tgcgccaggc ggcacaggcg ttcgccgagg tcgagaagtt gggccgagcg
    13921 gacggtccgc tcaatctggc ccgcgtctac atcaaggaag ggctggtgca gcagcaggcg
    13981 ccggcggcgc tcgcgcgcgc ggcggccaac gatccgccgg ccaacgagtg gtcgctgctg
    14041 tggttcggcg cggtgatcgc cgaggcgaac ggtgattacg acctcgcgat cgccaatctc
    14101 gagcgcatcg tgcgtggcgg gttcgagcag gcgcaaggac gcgacttcga gttccacaag
    14161 gactaccgcg tgcagaacca gctcggcaac gccctgtacc agcgtggcct gctcgagcag
    14221 ggagagacgc ggaagtccta cctcgagcgg gcgcgcgatc ggtttctcga ggcgttgagc
    14281 tacgaccccg agaacctgac ggcgcactgg ggccttcaac gggtctacgg tgacctcggc
    14341 accgcggaca aggcgcggga gcacgcggag ctgcatgcca agtacaaacc cgacgacaat
    14401 gcgcgggact acgccgcggc gcaggccagg ctgaagtacc ccgcggccaa ccgagcggcc
    14461 gaggccgtcg tgatctacga cctcgacgca cccgtcgaag tggacgatgc gctggaggta
    14521 gcgaaagttg aaaactgata ccgagggaca cgaccggacg accccggaag aagaatgggt
    14581 cccggaagac gacgcggtca tcggcaaggc gtttcgctgg tcgatgctgg tcggggcgat
    14641 cctggtgctc gtcgtgctgg ccaccctgtg gtcgctgcgc gagcggccgg aagtcgagac
    14701 gttcgtcgac agcgggccgg tcgccgtacc gcaagcgctg cgacaggaaa cggccgggat
    14761 gccgacggtc gccttcaccg acatcaccga gcgggccggg atcgatttcg tccacaacag
    14821 cggggcctac ggcgcgaagc tgctgcccga aacgatgggc ggtggcgtcg cgttcctcga
    14881 ctacgacaac gacggcgacg aggacctgct gttcgtcaac tcggccgact ggcccgacca
    14941 ccggcgcggc ggttcggcgc cgatgacgct gtaccgcaac gacggccggg gtcggttcga
    15001 cgacgtgacg cgagcgaccg gcctcgacgt cagcttctac gggcagggtg cggcggtcgg
    15061 cgactacgac aacgacggcg acgtcgacct gttcctgacg gcgctcggcg cgaaccacct
    15121 gttccgcaac gtcgacggtc gcttccatga cgtcaccgcc cgggccggcg tggccggcgc
    15181 cgacgatgac tggagtacga gcgccggctt cttcgactac gacatcgacg gcgatctcga
    15241 cctgttcgtc tgcaactacg tacggtggac tcgcgagatc gacatgcgcc tggcattcac
    15301 gctgaacggc accgatcgcg cctacggtcc gccgaagctc tatcagggag tgcactcctc
    15361 gctctatcgc aacgaggggg acgggacgtt ctccgacgtg tcgttcgaga tgggaatcca
    15421 ggtgaacaac ccggccaccg acaagccgat gggcaaagcg ctcgcggtga ccttcgccga
    15481 cgtcgaccac gacggctacc ccgacgtatt cgtcgccaac gacaccgtgc agaacttcct
    15541 gtttcgcaac aacggcgggc gttcgttcag cgagatcggt gcgatcagcg gtgtcggctt
    15601 cgacaaccag ggaaccgcga ccggcgcgat gggcatcgat gccgccgaca tcgccggtga
    15661 cggcaggctc ggcgtcgcga tcgccaactt ctccaacgaa acgacgtcgt tctacgtgca
    15721 gcaaaccgat ccgtggcagt tcgtcgacat ggcgaacgcg gtcggtgtcg gcccgccgag
    15781 ccggttgaag ctgagcttcg gtctgttctt cttcgactac gacctggacg gccggctcga
    15841 catgctgcag gccaatggcc acctggaaga cgagatcaac gagatccagc ccagccagca
    15901 ctaccggcag acgccgcagc tcttttggaa ctgcggttcg gacggcggga cctgtttcgc
    15961 cgccgtcgcc gacgagcagc tcggagcgct ggccacgccg atcgtcgggc ggggggcggc
    16021 gttcgccgac atcgacgccg atggcgacct cgatatcgtg ctggcgcaga ccgggggtcc
    16081 ggcgctgttg ctgcgcaacg accagcagct cggccgtcac tggcttcgcg tccgactggc
    16141 cggtaaccgc ggcaatcgcg acgcgatcgg cgccgggctc gagctgcgag ccggcgggcg
    16201 gacgcagaaa cgggtcgtca tgccgacccg cagctacctg tcgcaggtcg aacgaacggt
    16261 gacgttcggt ctcggcgaga acgaacgggt cgactcgctc gtcatccgct ggcccggcgg
    16321 ccgcacgcag caagtgaccg ttgggcaggt cgacacgacg ctggtcgtca acgaggggag
    16381 ttgaaccgag ccgggtgcgc ggaccgcggg ctcaagcgat ccacaccgtc ttgcggttga
    16441 cgaactcgag catgccctcg cgggacagct cccgcccgaa gccggactgt ttgacgccgc
    16501 cgaacggcag acgcgggtcg gacttgacca ggccgttgac gaagaccgat ccactgtcga
    16561 gccgcgggat cagccggtcg acccgctcgc gctcgcgcgt ccacagactc gcgcccagac
    16621 cgtactcgct gtcgttggcc agcgccaccg cctgccgctc gtcgtcgaac gggaacagcg
    16681 aggcgaccgg gccgaacagc tcctcgcggg cggcgggcga gccggccggg atgtcgacca
    16741 gcaccgtcgc cgggtagaag aacccgtcgc gcgccggcgg ctcgccgccg cacagcacgc
    16801 gcgcgccggc ggcgacggtc cgatcgacct ggtcggccag cgtgtcgcgc aggtcgcggc
    16861 gggccagcgg gccgagctcg acgctgcggt cccgcgggtc gccgatcttc cgcgaacgca
    16921 tccgttcgac gaccgcgtcg cggaagcggt cgaagaccga agcctcgacg agaaagcgct
    16981 tggccgcgat gcagctctgc ccgttgttca agcagcgcga cgcgacggcc gtgtcggccg
    17041 cgcgttcgag gtcggcgtcg ccgaagacga tgaacgggtc gctgccgccg agctccatca
    17101 ccatcgcctt cagttggcgc ccggccgcgg cggcgacctg tcgtccggct cgcgtgctac
    17161 cggtcagcgt caccccgcgc acccgggggt cggcgatcac cccggcggct tgctcgtccg
    17221 tcaggaacag gttctgcacg accccgtcgg gcagcccgga ctcgccgagc agtcgttcga
    17281 tccgtcgcgc gcaccccggg gtgttcggcg catgcttgag cagcacgacg ttgcccgcca
    17341 tcagcgccgg cgcggcgaag cggaagaact gccagaacgg gaagttccac ggcatgatcg
    17401 cgagcagcgg acccaacggg tcgtagcgca cgtaggcttg cgagccatcc gagtccttcg
    17461 catccggttc gaggaacgac gcggcctgct cggcgaagaa gcggcaaccc cacgcgcact
    17521 tggccgcctc cgcctcgccc tcgaccagcg gcttgcccat ctcgacggcc atcagttcgg
    17581 ccagctccgg cgcctggcgg tcgagcgact cggcgcagcg ccgcagccgc tcggcccggg
    17641 cctcgaccgg cgtggcacgc cacgcggcga acgccgccgc ggcccgttcg atcgcccccc
    17701 ggcattccgc gccggtggcg gtcgggtagc actcgagcgt cttgccggtc gtcggatcgg
    17761 tcgtttggta gtccaccggt gctcctcgat cgaccgctcc gcccgccggc tgatgctgac
    17821 cgattatacc gctcacaacc ggcgacgcag ctcgtcgacg ctcggcggac ggcgttccgg
    17881 cgacttgtcg agacagcgac ggatcgactc gctcagctcc accggggtgt cggccggcag
    17941 tcggcccagc tccgcctcgt cgcgcagcac ggcggcgatc gtgtcggatg cacagctccc
    18001 ggcgaaagcc cgatcgccgg tcagcatctc gaacaggatg cagccgagcg cccacaggtc
    18061 ggcgcgtgcg tcgaccggct cgccgcggat ctgctcgggg ctcatgtacc cgggcgtgcc
    18121 gagcatcgtc gatgcggcgg tgctgaaggc gcccgacccg tcccggtcgc gctcgaccgt
    18181 cttggccagg ccgaagtcga gcaccttgac ccggccggcg ggcgtgatca tcacgttggc
    18241 cggcttgagg tcgcggtgga cgatgccgtg agcgtgggcc tcggccaacc cgtcgcacag
    18301 ctcgagcgcg atgcggcgga cttcgcccgg cggaagcggc ccgccgcgca tcagctcgga
    18361 cagtgtcgtt ccgcggacga gctcgaggat caggtacggc tttcggtcga cgacgatcag
    18421 atcatggacc gtcgcgatgt tcgggtgatt cagtcgggcc aacagcttcg cttcgcgttc
    18481 gaaacggtcg agcgcgtcgc cggcggcgag cacgcccgac acggccttga ccgccac
//
LOCUS       DASPGN010000316         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_408233, whole genome shotgun sequence.
ACCESSION   DASPGN010000316 DASPGN010000000
VERSION     DASPGN010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408233"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..941)
                     /locus_tag="VD788_09230"
     CDS             complement(141..941)
                     /locus_tag="VD788_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76488.1"
                     /translation="MAFVGLLWPLVGLIGFVGYVWLCVVAFRRHVGWGLAVLFLSPVA
                     AVFFAIKNWTEARKPFLVYVMSLVMTVGLAVYTFSAFGGFKMMAMAEQIQNGELSEDE
                     AAVRMLEQMDRSGLLNDEQRAELKRMQQAVVEAKRQADAEPAPVHGSTVTPVSLPPPE
                     RVAAEPVATVDRSPRVDDDGSLDADPTYDDPAVELVPPGYRQIDPGEAGDYLGRDIRI
                     VNKDGSEHSGRLAEFESGVLLVERQLSSGTISFELSASDVDSLLVAYR"
     gene            complement(1030..1995)
                     /locus_tag="VD788_09235"
     CDS             complement(1030..1995)
                     /locus_tag="VD788_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXV76489.1"
                     /translation="MDVRRLATEAFARLAPCLPATPVEESPMLGRVVGGRVFLKLENR
                     QPSGSFKIRGVMNRLLAMPAERRRDGVVAASTGNHGLALAHASRVLAVPAVVFAPETA
                     RPAKIEALRASGVELRLSGEDCLDAERAARRFARERGAVYLSPYNDPYVVGGQATIGV
                     ELVDRPGPVDALVASLGGGGLIGGAGGYLKTEPTAALVIAGSPANSPAMHRSIEARRV
                     VATPSRPTLSDATAGDVEPGAITFELCRRTIDHSVLVDEDAILDGVRTIRREHGMWIE
                     GAAGVAVAAFFAAAALLRGARVVIVICGGNVDPSLAGRVAKITRK"
     gene            2130..>2913
                     /locus_tag="VD788_09240"
     CDS             2130..>2913
                     /locus_tag="VD788_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76490.1"
                     /translation="MGRIATVALTLVLAAPAGAAVAPDDPRPDPRERRPALARVESER
                     LALVPGDGRARAADRFRARYGGDWSFAYDRRSDHPALIQGAGIPLVPGRGNRLGGGAR
                     AGERVTLDTLAAEVRRLLAANGPLLVPAAGRLELDRAASSIREQGRLASLLFRWSVGG
                     VPVERAHVFVRINSGNVTQLGAPLVGRLTEDPVPAIDRERAIELLMRHSGDGEIYRMR
                     EAPALVFQPEEHDGRLDYRLVWKMSYRVLGRIETWEGRVDAHT"
BASE COUNT          427 a         1065 c         1025 g          396 t
ORIGIN      
        1 ggacgccgag tcgctccgcc gctgacccgc gagacgctcg agcgggcgca tcagcgaccg
       61 ggcaacggca acccgtcgat ctggtggatc gatgcgagcg tcatcgaccg gctggccgcc
      121 ggcgccggca gccggatcga ctagcggtag gcgaccagca gcgagtcgac gtcgctcgcg
      181 ctcagctcga acgagatcgt cccgctcgac agctgtcgct cgacgagcaa caccccgctt
      241 tcgaactcgg ccagccgccc gctgtgctcg ctgccgtcct tgttgacgat gcggatgtcg
      301 cggccgagat agtcgccggc ctcgcctggg tcgatctgtc gatagccggg cgggaccagc
      361 tcgaccgccg ggtcgtcgta ggtcggatcg gcgtcgagcg agccgtcgtc gtcgacgcgc
      421 ggcgagcggt cgaccgtcgc cacgggctcg gccgcgaccc gctccggcgg cggaagggat
      481 accggggtga cggtcgagcc atgcacgggc gcgggctcgg catcggcctg ccgtttcgcc
      541 tccacgaccg cctgctgcat tcgcttcaac tcggcccgtt gctcgtcgtt cagcagcccg
      601 gatcggtcca tctgctcgag catgcgaacc gcggcctcgt cctcgctcag ctcgccgttt
      661 tggatctgct cggccatcgc catcatcttg aacccgccga acgccgagaa ggtgtagacc
      721 gccaggccga ccgtcatcac caagctcatc acgtagacga ggaacggctt gcgtgcctcg
      781 gtccagttct tgatcgcgaa gaagacggcc gccacgggtg agaggaacag gacggcgaga
      841 ccccagccga cgtggcgcct gaacgccacg acgcacagcc agacataacc gacgaaaccg
      901 atcaagccga ccaacggcca cagcaaaccg acgaatgcca tcgcgcgcgc tcctgtcgcc
      961 ccgccctcga tcaaacctag gtcggcgcgc ggcgaaggca accccggcag ggtccccgcc
     1021 cgagtccggt cattttcgcg tgatcttcgc cacccggccg gcgagcgacg gatcgacgtt
     1081 accgccgcag atcacgatca ccacgcgggc gccgcgcaac agcgccgcgg cggcgaaaaa
     1141 cgccgcgacg gccaccccgg cggcgccctc gatccacatc ccgtgctcgc gccggatcgt
     1201 tcgcacgccg tcgaggatcg cgtcctcgtc gacgagcacc gagtggtcga tcgtccgccg
     1261 gcagagctcg aaggtgatcg caccgggctc gacgtctccc gcggtcgcgt ccgacagcgt
     1321 cggccgcgag ggggtggcga cgactcgccg tgcctcgatc gatcggtgca tcgccggcga
     1381 gttcgccggc gacccggcga tgacgagggc tgccgtcggc tcggtcttga gatagccgcc
     1441 cgccccgccg atcagcccgc cgccacccaa cgaggcgacg agcgcgtcca ccggaccggg
     1501 ccggtcgacc agctcgaccc cgatcgtcgc ctggccgccg accacgtacg ggtcgttgta
     1561 cggcgaaagg tagacggccc cccgttcgcg ggcgaatcga cgggcagcac gctcggcgtc
     1621 gaggcagtcc tcgccggaga gtcggagctc gacgccgctc gcgcggaggg cttcgatctt
     1681 ggccggccgc gccgtctcgg gtgcgaagac gaccgccggc accgcgagca ctcgcgacgc
     1741 gtgggccagc gccaggccgt ggtttccggt cgaggcggcg acgacaccgt cgcggcgccg
     1801 ctcggcaggc atcgccagca ggcggttcat cacgccgcgg atcttgaacg agccgctcgg
     1861 ctgacggttc tcgagcttga ggaagacacg gccgccgacc acgcgcccca gcatcggcga
     1921 ctcttcgacc ggcgtcgccg ggaggcacgg cgcgagccgg gcgaacgcct ccgtcgcgag
     1981 ccgccgaaca tccatcgcgc atcccaggct atactcggtc gtccgtcggg gccagcgtcc
     2041 gagggcgcgg tcctcagcgc cgctggccga gaggtcgcac acggcgtaga agaaggcgtg
     2101 tgccggcctc gtcccggggg gagggtgtgt tgggtcggat cgcgacggtc gcactgacgc
     2161 tggtgctcgc cgcgcccgcc ggggcggcgg tcgccccgga cgatccgcga cccgaccccc
     2221 gcgagcggcg gccggcactc gcccgagtcg aaagcgaacg gctcgccctc gtccccggcg
     2281 acgggcgcgc ccgcgcggcc gatcgcttcc gcgcgcgcta cggcggcgac tggagcttcg
     2341 cctacgaccg ccggtcggat cacccggcgc tgatccaggg tgccgggatc ccgctcgttc
     2401 ccggtcgggg taaccggctc ggtggcgggg cccgggccgg cgagcgcgtc acgctcgaca
     2461 cgctcgcggc ggaggtccgg cggttgctcg cggccaacgg cccgctgctc gttcccgccg
     2521 cgggacggct cgagctcgat cgtgccgcca gctcgattcg cgagcagggc cgcctggcga
     2581 gcctgctttt tcgctggtcg gtcggcggcg tgccggtcga gcgggcccac gttttcgtcc
     2641 ggatcaactc gggcaacgtc acgcagctcg gcgctccgct cgtcgggcgg ctgaccgaag
     2701 accccgtgcc ggcaatcgac cgcgagcgcg cgatcgagct gctgatgcgg cacagcggcg
     2761 acggcgagat ctaccggatg cgcgaggcac cggcgctggt ttttcagccg gaggaacacg
     2821 acggccgtct cgactaccgg ctggtatgga agatgagcta tcgcgtgctg gggcggatcg
     2881 agacgtggga ggggcgcgtc gacgcgcaca ccg
//
LOCUS       DASPGN010000317         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1111176, whole genome shotgun sequence.
ACCESSION   DASPGN010000317 DASPGN010000000
VERSION     DASPGN010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111176"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VD788_09245"
     CDS             complement(<1..684)
                     /locus_tag="VD788_09245"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HXV76491.1"
                     /translation="MRHAPPRFAPFARIAPCLAVACATALAPAADRAPSAADTDADRI
                     AAPRLLWDSGGGLLALPLADVDVELTVTGVMAHGKLTQRFDNPTGEVIEAVYVFPMPD
                     RAAVYRMEMRVGDRRIVAVVHEKEEARQDYDRAKQTGRKASLVDEHRPNLFSTSVANI
                     NPGETVRVTLEYYEEVDYRDGEFGLRFPLSFTPRYDPARGEEIAGVDDPFATGVGLPV
                     VRLGVTLRPG"
     gene            916..2061
                     /locus_tag="VD788_09250"
     CDS             916..2061
                     /locus_tag="VD788_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HXV76492.1"
                     /translation="MRDDVLTMIPGPTPVRRTILDALARPTTSHQAPSFVETFREALD
                     QVKTIVMSAVGYPFVVSGSGTLAMEMALVNLVASDERLLVVSQGYFGDRWAQLAQAFG
                     IECRLLQAEWGRQVPVERVRREVEHGRYAAVAMTHVDTSTGTAAPVEEYCRALAGRET
                     LLLLDGVCATGGIEERFDEWGLDVVLTGMQKALGAPPGLAILVVSERALARRRAIDAV
                     PAYYADLLRWEPVMEDPARYFSTPPVNEVLALHEAARLVLNEGLARRFARHDRVAAAM
                     RRGFERLGLTLFTDERCRASTLTVLNYPDRVEDAPFRAVVATHGVVVAGALGPIAGKA
                     FRVGHMGNIGAAEVGRTVAALGRALDEVGRPVDVDGAVAEALAGLEP"
     gene            complement(2066..3421)
                     /locus_tag="VD788_09255"
     CDS             complement(2066..3421)
                     /locus_tag="VD788_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXV76493.1"
                     /translation="MARSPARRNEIGAMLRLAIPVVLAEIGWVAMGVVDVMMVGRIDA
                     ESIGAVSVGRAAFMLVCVFGIGLLLGLDTVVSQAYGAGDERECRLSLLHGVYLALIVT
                     VPLMLVAGLLGLLIDRWPMDERVRRMAVGYLAGVNWSLLPVLLYVAFRRYLQAMNLVR
                     PVMIALLSANLVNVLANWVLIFGHLGAPPLGAVGAAWATVLASAYMALFLLVAIVLHH
                     RDERSVLRSIPWRLDPRRLGRLIGLGLPAGGQLLLEIGVFALATVLAARLAPSALAAH
                     QIAINVASVTYMVPLGMSSAAAVRVGQALGRSDREGAASAGWTALGLGSAFMALAALV
                     FVTFPVRIVGAFTSEAEVLRAGVRLLYVAAVFQLFDGLQVVAIGALRGSGDTRTPMVW
                     NLVGYWLMGLPVGYYLCFERGLGVLGLWIGLSLGLIVVGTILLRTWARLTVSWRGTAD
                     R"
     gene            3433..>3884
                     /locus_tag="VD788_09260"
     CDS             3433..>3884
                     /locus_tag="VD788_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76494.1"
                     /translation="MEKPIRRRRAGQPAQDSEPSSNGATLAGLFLGGLVLRLVHVWTV
                     RESPLFTMPMLDPAMYDEWARALLAGRPPGGVFFQDPLYAYVLAGLYRTLGDGPLVVA
                     IVQSILGAAVAPLVFVAARPSFGVATAGTAGSLAALYAPAIYYDGMLL"
BASE COUNT          610 a         1365 c         1376 g          533 t
ORIGIN      
        1 accgggacgc agcgtgacgc cgagacgcac gaccggcagg ccgactcccg tcgcgaacgg
       61 gtcgtcgacg ccggcgatct cctcgccgcg tgccgggtcg taccgcgggg tgaagctcag
      121 cggaaagcgc agcccgaact cgccgtcccg gtagtcgacc tcctcgtagt actcgagcgt
      181 cacgcgcacc gtctcgcccg ggttgatgtt ggcgaccgag gtcgagaaca ggttcggccg
      241 gtgttcgtcg acgagcgagg ccttgcgtcc ggtctgcttg gcccgatcgt agtcctgtcg
      301 ggcctcttcc ttctcgtgca cgacggcgac gatgcgccgg tcaccgaccc gcatctccat
      361 gcggtagacc gccgcgcggt cgggcatcgg gaagacgtag accgcctcga tcacctcgcc
      421 ggtcggattg tcgaagcgct gggtcagctt tccgtgcgcc atcaccccgg tgaccgtcag
      481 ctcgacgtcg acgtcggcca agggcagcgc cagcaggccg ccgccgctgt cccacagcag
      541 gcggggagcc gcgatccgat cggcgtctgt gtcagccgcc gacggcgcgc ggtcggcggc
      601 gggagcgagg gccgtcgcgc aggcgacggc gagacaggga gcgatacggg cgaacggggc
      661 gaatcgtggt ggggcgtgac gcatggcgcg tttctccgac ggccccaccc gtatcgggcg
      721 acaaggcacg atccgtgcca cgcagacgaa tcgaaataac cggcgcgacg ctcgaggtcg
      781 gtgcgagtcg acgtgtcggc cgtgccacgg ctcgtgtacg gcgaccgacg tcgagtcggc
      841 gcagccgctt tacccgcgca ccgggttggt tcatcatcga ggctcggatc ttccgacggg
      901 cggaggcgac ttgcgatgcg cgacgacgtg ttgacgatga tcccggggcc gaccccggtt
      961 cgccggacga ttctcgatgc gctggcgcgg ccgacgacct cccaccaggc gccgtcgttc
     1021 gtcgagacgt ttcgcgaggc gctcgaccag gtcaagacga tcgtgatgag cgcggtcggc
     1081 tacccgttcg tggtgtcggg gtcgggcacg ctggcgatgg aaatggcgct ggtcaacctc
     1141 gtcgcgagcg acgaacgact gttggtcgtc tcccaggggt acttcggcga ccgctgggcc
     1201 cagctggccc aggccttcgg catcgagtgt cgcctgctgc aggcggagtg gggacggcag
     1261 gtgccggtcg agcgggtgcg ccgtgaggtg gagcacgggc gttatgccgc ggtggcgatg
     1321 acccacgtcg acacctcgac cggtacggcg gcgccggtcg aagagtactg ccgcgcgctt
     1381 gccggccgcg agacgctgtt gctgctcgac ggggtctgcg cgacgggcgg gatcgaggag
     1441 cggttcgacg agtgggggct cgacgtcgtg ttgaccggca tgcagaaggc cctcggcgct
     1501 ccccccggcc tcgcgatcct cgtcgtctcc gagcgcgcgc tcgccaggcg acgcgcgatc
     1561 gatgcggtgc cggcgtacta cgccgatctg ctgcgctggg aaccggtgat ggaggatccg
     1621 gcccgctact tttcgacgcc cccggtcaac gaggtgctgg cattgcacga ggcggcgcgc
     1681 ctggtgctga acgagggtct cgcgcggcgt ttcgcgcggc acgaccgtgt cgccgcggcg
     1741 atgcgccgcg ggttcgaacg gctcggtctg accctcttca ccgacgaacg gtgccgggca
     1801 tcgacgctga ccgtgctgaa ctacccggac cgggtcgagg acgcgccgtt ccgcgccgtc
     1861 gtcgccacac acggcgtcgt cgtcgccggt gcgctcggac cgatcgccgg caaggcgttc
     1921 cgggtcgggc acatggggaa catcggcgcg gcggaggtcg gacgcacggt cgccgcgctc
     1981 ggccgtgcgc tcgacgaggt cggccggccg gtcgacgtcg acggcgccgt cgcggaggcg
     2041 ttggccgggc tcgagccgtg agcgatcagc ggtccgccgt cccgcgccag ctcacggtga
     2101 gacgggccca ggtccggagc aggatcgtgc cgaccacgat caggcccagc gacagaccga
     2161 tccacagacc gaggacgccg agcccccgct cgaagcagag gtagtaaccg accggcagcc
     2221 ccatcagcca gtagccgacc aggttccaca ccatcggtgt ccgcgtgtct cccgacccgc
     2281 gcaacgcgcc gatcgccacg acttgcagac cgtcgaacag ctggaacacg gccgcgacgt
     2341 acagcagtcg gacgccggcc cggagcacct cggcctccga cgtgaacgcg ccgacgatcc
     2401 ggacggggaa ggtgacgaag accagcgccg cgagcgccat gaacgccgag cccaagccga
     2461 gcgcggtcca gccggcgctt gccgccccct cccggtcgga gcggccgaga gcctgaccga
     2521 cgcggaccgc ggccgccgac gacatgccga gcggaaccat gtaggtgacg ctcgccacgt
     2581 tgatcgcgat ctgatgcgcg gcgagtgccg agggcgcgag gcgcgcggcg agcaccgtgg
     2641 caagggcgaa gaccccgatc tcgagcaaca actgaccgcc ggcgggcagc ccgagcccga
     2701 tcagccgtcc caagcgccgc gggtcgagtc gccacgggat cgagcgcagc accgagcgct
     2761 cgtcacggtg gtgcagcacg atcgcgacca gcaggaacag cgccatgtag gcgctcgcca
     2821 gcacggtggc ccacgccgcg ccgaccgcgc cgagcggggg ggcaccgaga tggccgaaga
     2881 tcaacaccca gttggccagg acgttgacca gattggccga cagcagcgcg atcatcaccg
     2941 gccgcacgag gttcatcgcc tgcaagtaac ggcggaacgc gacatagagc agtaccggca
     3001 gcagcgacca gttgaccccg gccaggtagc cgacggccat ccggcgcacc cgctcgtcca
     3061 tcggccagcg gtcgatcagc aggccgagca ggcccgcgac cagcatcagc ggcacggtga
     3121 cgatcaacgc cagatagacc ccgtgcagca ggctgagccg gcactcgcgc tcgtcgcccg
     3181 cgccgtacgc ctgcgacacg actgtatcga ggcccaacag cagcccgatg ccgaagacgc
     3241 agaccaacat gaaggccgcc cgtccgacgc tcaccgctcc gatcgactcg gcgtcgatgc
     3301 ggccgaccat catcacgtcg acgacgccca tcgcgaccca gccgatctcg gccagcacca
     3361 cgggaatcgc caggcgcagc atcgcgccga tctcgtttcg ccgagcgggg gatcgtgcca
     3421 tgatggaccg tcgtggaaaa gccgatccgc cgacggcgcg cgggccagcc agcccaagat
     3481 agcgaaccat cgtcgaacgg cgccacgctc gccggcctgt tcctcggcgg gctggtgctg
     3541 cgactcgtcc acgtctggac ggtgcgcgaa tcgccgctgt tcacgatgcc gatgctcgat
     3601 cccgcgatgt acgacgagtg ggcgcgggcg ctgctcgccg gtcgcccgcc gggcggcgtc
     3661 ttctttcagg atccgctcta cgcctacgtc ctggcggggc tgtaccggac gctcggcgac
     3721 ggtccgctcg tggtggcgat cgtccagtcg atcctcggcg ccgcggtcgc accgctcgta
     3781 ttcgtcgccg cccgcccgtc gttcggcgtc gcgacggccg gaacggccgg ctcgctcgcg
     3841 gcgctctacg cgccggcgat ctactacgac ggaatgctgt tgaa
//
LOCUS       DASPGN010000318         3123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1461442, whole genome shotgun sequence.
ACCESSION   DASPGN010000318 DASPGN010000000
VERSION     DASPGN010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461442"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1938
                     /locus_tag="VD788_09265"
     CDS             <1..1938
                     /locus_tag="VD788_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76495.1"
                     /translation="LSVPPGLDRIMRRCLEKAPDLRFQSADDLAFALETLSTASGPTG
                     AAAIAAPARAGRSQVLPWLLVTVLAAVAAAGWLRRGASVPTALPFNTFTRITELAGEE
                     TDPSLSPDGSIVAYAVRESDSWDIYTQRVGGRNATAILNDPQRNERAPAFSPDGAWIA
                     LHESDTDGGIFVAGATGESVRRVTDFGFHPAWSPDGRRLAFTSEEIFDPSARQGAAGL
                     YVVDAGGGAPRKVVEGDAAQPSWSPSGHRLLYWSNTAGQRDLFTVSSAGGDRVPLTND
                     PPIDWSPVWSPDGRYAYFASDRGGAMNLWRIAVDQDTGAPQGYAEPVTMGAQASAGLP
                     SFSKDGTRLAFRSRIGSVNPIEIPFDPVALRAGEPRLLDSRTNIRVPGSVSPDGSLVA
                     YFSIGDRQEDLFVGPPNGSMRRVIDDAPRDRGPEFTPDGRSLVFYSNRSGQWQLWIVG
                     IDGSGLRQVGDAPDGAVYPVVSPRGDTIAFAGSSTRNAYTMPLADSAVPTLLAGVLIG
                     DDLLTPFSWSRDGTRLAGVLVSPSGGPVGVGVYDLATGTATRLTSDPTYAVRWLADSR
                     RVVYFTNGGWQLVVLDTVSRTRTPIALRLPAPSTIDLFALSPDDRHIYYGAARAEADI
                     WILEQKREPRAPRATSATINR"
     gene            1994..2944
                     /locus_tag="VD788_09270"
     CDS             1994..2944
                     /locus_tag="VD788_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76496.1"
                     /translation="MRGLPSVARRLGIAASLLLAGIPAAGEPVTFVFKAEVTNVFDGL
                     GALGGRVEPGTIVRGTYTFDSETPNTAEPVDEGELGLYHHDRPPARVRLFAGPFVFRS
                     VPSVPVVDIHVANDFGFAGTDEYGFTSANNQARGLYPSAPVDRIDLAWRAQNFSGQPF
                     TSAALPLTPPDLTILGGGLLTIYGECTLCAGPAAFFRIEGRLTSLRRAVHMVVDHDAL
                     RCDPNPAYEIAGFYDLVRGDLAALRANGGDFAAAIDECLADDAEGAAFPYALAPEPGQ
                     GFWFLSRRASDALGETYDSGGLGQLQDRDEPIAVSGVDCP"
BASE COUNT          435 a         1125 c         1075 g          488 t
ORIGIN      
        1 ctgtcggtgc cgcccggtct cgatcgcatc atgcggcggt gcctggagaa ggcgccggat
       61 cttcgattcc agtccgccga tgatctcgcg ttcgcactgg aaacgctgtc gaccgcatcc
      121 ggaccgaccg gagccgcggc catcgcggcg ccggcccggg ccggtcgatc gcaggtcctg
      181 ccctggctgc tcgtgacggt cctggcggcg gtcgcggcgg ccgggtggtt gcggcgcggc
      241 gcgtccgtcc caacggccct gccgttcaac accttcacgc gcatcacgga gctcgccggc
      301 gaagaaaccg acccgtcgct ctcgcccgac ggatcgatcg tggcctatgc cgtccgtgag
      361 agcgacagct gggacatcta cacgcagcgc gtcggcgggc gcaacgcgac ggcgattctg
      421 aacgacccgc aacgcaacga gcgcgcgccg gccttttcgc ccgacggcgc gtggatcgcc
      481 ctccacgagt cggacaccga cggcggcatc ttcgtcgccg gcgcgaccgg cgagtccgtg
      541 cgccgcgtca ccgatttcgg gttccatccg gcctggtcgc ccgacggccg tcgcctggcg
      601 ttcacctccg aggagatctt cgatccgtcc gccaggcagg gcgcagccgg tctgtacgtc
      661 gtggatgccg gcggcggcgc gccgcgcaag gtcgtcgagg gcgacgcggc ccagccctcc
      721 tggtcgccgt cgggccaccg gctcctctac tggagcaata ccgccggcca gcgcgatctg
      781 ttcacggtca gttcggccgg cggcgatcgg gtgccgctca cgaacgaccc gcccatcgac
      841 tggtcgcccg tctggtcgcc cgacggccgg tacgcgtact tcgcgagcga ccgcggcggc
      901 gcgatgaacc tgtggcgcat tgcggtcgat caggacaccg gcgcgccaca gggctacgcg
      961 gagccggtaa cgatgggagc gcaggcgtcg gccggcctgc cgtcgttttc gaaggacggc
     1021 acgcggctgg ccttccggtc gcgcatcggg tcggtcaatc ccatcgagat cccgttcgat
     1081 ccggtggcgc tccgcgcggg agagccgcgc ctcctcgatt cccgaacgaa tattcgcgtg
     1141 cccggcagcg tgtccccgga cggttcgctc gtcgcctact tcagcatcgg cgatcgacag
     1201 gaggatctct tcgtcggccc accgaacggc tccatgcggc gcgtgatcga cgacgccccg
     1261 cgggaccggg gtccggagtt cacgccggac ggccgttcgc tcgtgttcta ttcgaaccgc
     1321 tcgggccaat ggcagctgtg gatcgtcggc atcgacggca gcgggcttcg ccaggtgggc
     1381 gacgcgcccg acggcgccgt gtacccggtg gtgtcgccgc ggggcgacac gatcgccttc
     1441 gccggctcgt cgacacgaaa cgcgtacacg atgccgctcg cggacagcgc cgtgccgacg
     1501 ctactggcgg gggtcctgat cggcgacgat ctgctgacgc cgttctcgtg gtcgcgcgac
     1561 ggcacgcgcc tcgcgggcgt tctggtttct ccaagcggcg gtccggtcgg cgtaggcgtg
     1621 tacgatctcg cgaccggaac ggcgacgcgg ctgacgtcgg acccgacgta cgccgtgcgg
     1681 tggctggccg acagtcggcg ggtcgtctac ttcacgaacg gcggctggca gctggtcgtg
     1741 ctcgacacgg tctcgcgcac gcgcacgccg atcgcgttgc ggctcccggc gccatcgacc
     1801 atcgacctct tcgccctcag cccggacgac cgccacatct actacggcgc cgcgcgcgcc
     1861 gaggcggaca tctggattct ggagcagaag cgtgagcctc gagcgccgcg agcaacgagc
     1921 gcgacgatca accgttgagt cgggtggggc atctcggggg ggcagcgtat actcccggcc
     1981 aaggaggcgg acgatgcgag gacttccatc cgtcgcgcgc cggctcggca tcgcggcgag
     2041 cctgctcctt gccggaattc ccgcggccgg cgagccggtc accttcgtct tcaaggccga
     2101 ggtcacgaac gtcttcgacg gactcggcgc gctcggcggc agggtcgagc cgggtacgat
     2161 cgtgcgcggc acctacacct tcgattccga gacgcccaac acggccgagc cggtcgatga
     2221 aggggagctc ggactgtacc accacgatcg cccacccgcg cgcgtgcgcc tattcgccgg
     2281 gccgttcgtc ttccgtagcg tcccgtcggt gccggtcgtc gacatccacg tcgcaaacga
     2341 cttcgggttc gccggaaccg acgagtacgg cttcacgagc gcgaacaacc aggcgcgcgg
     2401 cctctacccg agtgcgcccg tcgaccggat cgacctcgcc tggcgcgccc agaacttcag
     2461 cggccaaccc ttcacgagcg ccgcgctgcc gttgacgccg cccgacctca cgatcctcgg
     2521 gggcggcctg ctcacgatct acggcgagtg cacgctgtgc gcggggccgg cggccttctt
     2581 ccggatcgag gggcggctga cctcgctgcg gcgggccgtt cacatggtcg tcgatcacga
     2641 cgcgttgcgc tgcgacccga atccggccta cgagatcgcg ggcttctacg acctcgtgcg
     2701 cggcgacctt gcggcattgc gggcgaacgg cggcgacttc gcggcggcaa tcgacgaatg
     2761 cctcgccgac gacgccgagg gtgccgcgtt cccctacgcg ctcgcgcccg agccgggcca
     2821 aggcttctgg ttcctctcgc gccgcgcgtc ggacgcgctc ggagagacct acgactccgg
     2881 aggtctcggg cagctccagg accgcgacga gccgatcgcg gtctccggag tcgactgtcc
     2941 gtagcgcggt ggcggtggcc aggaaactcc aactgcgttt cggcttggct tgggtccgag
     3001 atgagtgatt atgtcgactc gaatcgtgcg ggcaaaccgg ccccccagag cgatccatcc
     3061 gccagacacc acccgcgcga taacgagcgt cgtccggcgg cgcccgacgg cgccgtctat
     3121 ttc
//
LOCUS       DASPGN010000319         6635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_292856, whole genome shotgun sequence.
ACCESSION   DASPGN010000319 DASPGN010000000
VERSION     DASPGN010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292856"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..644)
                     /locus_tag="VD788_09275"
     CDS             complement(210..644)
                     /locus_tag="VD788_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76497.1"
                     /translation="MVRTTVWTLTLAIACVAAGTASAAMLSKTYEFNPDAILEIGAST
                     GDGLRLDTVRFQMPTTTGVRTASEASVEIAISNTSEESKRVGIGVALFDSEGRLVGVA
                     SGGTRLVGLKPDKQKSYKLIFADLNVEAPRAATFQISVESRP"
     gene            complement(637..1125)
                     /gene="moaC"
                     /locus_tag="VD788_09280"
     CDS             complement(637..1125)
                     /gene="moaC"
                     /locus_tag="VD788_09280"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HXV76498.1"
                     /translation="MSGKLTHLDGKGRASMVDVSGKPVTARVARAEGRVRLNAAAFRA
                     FADDRLAKGDAAAVVRLAGIQAAKRTDEWVPLCHAIPLDHLSIELEIDEEARTVHIVA
                     EARARWSTGVEMEALVAVSGACLALYDMTKALDRGITIEQVRLIEKSGGRAGHWKRSG
                     DG"
     gene            complement(1122..1733)
                     /locus_tag="VD788_09285"
     CDS             complement(1122..1733)
                     /locus_tag="VD788_09285"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HXV76499.1"
                     /translation="MPQTVGVVLAGGRGDRLGRTKGDLAIDGNSLAERAASLLWPLCG
                     SVLISIADGMANPAPDYPVVVDRPPPGRGPLVGIDAAFAATGDADLLVLACDYPRVSQ
                     SLMRRLASHAAATDDLVMMTDFAGRDHPLVGLWRRAVAGVVRRAVETGRYKVRALLLE
                     LRVRRIGPAEFPGVDLDRVLLNVNSPEELARLSPPESRPEWPR"
     gene            1785..2462
                     /locus_tag="VD788_09290"
     CDS             1785..2462
                     /locus_tag="VD788_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXV76500.1"
                     /translation="MHWLLLFGKRPRRGRVKTRLVPPLTDEHALRLYRAFLVDQLRFV
                     RGFHGVRAAWVADGPLDAASDRELPIDGVERLEQGEGDLGARLRRAFAAATRRGAGPT
                     VVIGTDSPTLPETHVLRAFELLESGAPAVMAPSYDGGYVLIGCVGSRSELFERVPWGG
                     PAVAETTRRRAVQAGIALHTIDPWYDVDDREGLDRLRRELRDPSAARRAPRTARALLD
                     LPFDPVV"
     gene            2629..4068
                     /gene="rho"
                     /locus_tag="VD788_09295"
     CDS             2629..4068
                     /gene="rho"
                     /locus_tag="VD788_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXV76501.1"
                     /translation="MSEERPRGRRGPTRGDRRGGRRRGPSAPGTNLREADEVALPEAE
                     TEGKVAVAEGPPDDDNVVDLNSLYKMDNHALVSAAKDLGIGGSTSLRKQELIFEIMKA
                     QSERSGLVFAEGVLQILQDGYGFLRHPDYNYLPGPDDIYVSPSQIKRFGLTTGDTVSG
                     QVRPPKHDENYFALIKVLAVNFDDPERIRERIFFDNLTPLYPLDHIRLETDAENVAGR
                     IMDLLTPIGKGQRGLIVSPPRTGKTMLLQSLANSITRNHPEAFLIVLLIDERPEEVTD
                     MQRSVQGEVVASTFDEPAQQHVHVAEMVIEKAKRLVEHGKDVVILLDSITRLARAYNT
                     VQPPSGKVLSGGIDANALQRPKRFFGAARNIEEGGSLTIMATALIDTGSRMDDVIFEE
                     FKGTGNMEIHLDRKLVDRRVFPAIDIERSGTRKEELLLGKETLNKVWILRKILNQMSA
                     VEAMELLIDKLTKSESNAEFLKLMQNPTG"
     gene            complement(4079..6226)
                     /gene="recG"
                     /locus_tag="VD788_09300"
     CDS             complement(4079..6226)
                     /gene="recG"
                     /locus_tag="VD788_09300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXV76502.1"
                     /translation="MAYYSDRAMSTIRADTELRFVRGVGPRRAEKLSRLGYETVEDLL
                     YVLPFRWEDRRAFAAVRELRPEAGECTVSVKVERSRLIRTRRRGFTIFEATVADASGV
                     IRAVWYNQPYLERLLTAGREVVLYGRASIDRSGRPTLDNPEYEFVEPGDSEGIHAGRV
                     VPVYRKLADLSSRALRAAIHRALSDLAPAGLRELIPLEVARAHGLIERRRALREIHFP
                     PDEVALDELAAGRTPGQRTLAFEEIYLLQLALALRRHGLEQQGRGIAYAVPDAFRTRL
                     ARMLPFKLTAAQRRVLREIGSDLTSDHPMNRLLQGDVGSGKTVVALLTLLVAIENGYQ
                     GALMAPTEILAEQHFRNLRSMLEPSEIDCRLELLTGSLRAPQRKRVLERIASGEAQLA
                     VGTHALFEAGVGFRRLGLVVIDEQHRFGVLQRAALARKGRHPDVLVMTATPIPRSLAL
                     TLYGDLDLSVIDELPPGRKEIRTLVRAESDRRKVYDGLRREVQRGRQAYVVVPLVAES
                     ERVDLKAATEFADHLAAEVFADLRVGMMHGRLKPADKDRIMQEFREGRIQVLVSTTVI
                     EVGVDVPNATVMLIEHAERFGLSQLHQLRGRVGRGAARSYCVLMVGREQAGEDARERL
                     EVMERTNDGFQIAERDLHIRGPGVVFGTQQHGLSDLQFLAQVVKTPELLEAARREARA
                     LVAAGEPSREAARRMLVGLGGKWRRRLELAQVG"
     gene            6347..>6635
                     /locus_tag="VD788_09305"
     CDS             6347..>6635
                     /locus_tag="VD788_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV76503.1"
                     /translation="MLGDLPRGTLREVVVVDNGSTDRTAEVARAAGATVVSENRRGYG
                     SACLAGVAHLARLSPAPQIVVFVDADYSDHAEELPALIAPIVEDRADIVVGS"
BASE COUNT         1051 a         2374 c         2200 g         1010 t
ORIGIN      
        1 tggcgctcga gcgcgcgccg ggtggggttg accgtgcgac cgtaggtgta gggccaaccc
       61 tgctcgaggc ccggctgccg gtaggtcgag gtcagataca gcggttgcat gaccgccccg
      121 gtcgtcgggt cggcttgctg accagcgtgg accacgagcg aatcgaagcg aagggggtgg
      181 gtcctgcggg ccatcgccgg tggcacgctt cagggtcgcg attcgacgct gatctggaac
      241 gtcgcggccc gcggtgcctc gacgttgagg tcggcgaaga tcagcttgta cgacttctgc
      301 ttgtctggtt tgagcccgac gaggcgcgtc ccgccgctgg cgactccgac cagccgcccc
      361 tcggagtcga acagtgcgac cccgatcccg acccgcttgc tctcctcgct cgtattcgag
      421 atcgcgatct cgacgctcgc ctcgctcgcc gtgcggactc cggtcgtcgt cggcatctgg
      481 aatcgaacgg tgtccaaccg caggccgtcc ccggtcgaag ccccgatctc gagtatcgcg
      541 tcgggattga actcgtaggt cttcgacaac atcgccgccg aggccgttcc cgccgcgacg
      601 cacgcgatcg ccagcgtcag ggtccacacg gtcgtcctaa ccatcaccac tccttttcca
      661 gtgtccagct cgcccgccgc tcttctcgat caggcgcacc tgctcgatcg tgatccctcg
      721 gtcgagcgcc ttggtcatgt cgtacagcgc cagacaggcg ccgctgacgg cgacgagggc
      781 ttccatctcc actccggtcg accatcgggc gcgcgcttcg gcgacgatgt gtacggtccg
      841 cgcttcctcg tcgatctcga gctcgatcga cagatgatcg agcggaatcg cgtggcacag
      901 cgggacccac tcgtcggtcc gtttggcggc ctggatgccg gccagccgca ccaccgcggc
      961 ggcgtccccc ttggccagtc gatcgtcggc gaacgcgcga aacgccgccg cgttgagccg
     1021 cacccgaccc tcggcgcggg ccaccctggc cgtcaccggt ttgcccgaca cgtcgaccat
     1081 cgacgcccgt cccttgccgt cgagatgcgt cagcttgccg ctcatcgcgg ccactccgga
     1141 cgcgactcgg gtgggctcag ccgggccagc tcctccgggg agttgacgtt gagcagcacg
     1201 cggtcgaggt cgacgccggg aaactcggcc gggccgattc gtcgcaccct cagctcgagc
     1261 aggagggcgc ggaccttgta gcgcccggtc tcgaccgcgc gtcgcaccac gccggccacg
     1321 gctcggcgcc acaacccgac cagcggatga tcgcgaccgg cgaagtcggt catcatcacc
     1381 agatcatcgg tcgccgccgc gtgcgaagcc aaccgccgca tcagcgactg ggagacgcgc
     1441 gggtagtcgc aggccagcac cagcaggtcg gcatcaccgg tcgccgcgaa cgccgcgtcg
     1501 atcccgacga gcggcccgcg gccgggcggc gggcgatcga cgacgaccgg gtagtcgggc
     1561 gccgggttcg ccatcccgtc ggcgatgctg atcagcacgc tgccacacag cggccacagc
     1621 aggctcgcgg cgcgctcggc cagcgagttg ccgtcgatcg ccagatcgcc cttggttcgt
     1681 ccgagacgat cgcctcgacc gccggcgagg acgacaccca ccgtctgagg catggctccc
     1741 ctccgcggcg cattctagcc gacgaccgct agaatgcacc cgcgatgcac tggttgctgt
     1801 tgttcggcaa gcgaccccgg cgaggtcgcg tcaagacccg cctggttcct ccgctcaccg
     1861 acgagcacgc gctgcgcctg taccgcgcgt tcctcgtcga tcagctccgg ttcgtccgcg
     1921 ggttccacgg cgttcgcgcg gcctgggtcg ccgacgggcc gctcgatgcc gcgtcggacc
     1981 gcgaattgcc gatcgacggg gtcgagcggc tcgaacaggg cgaaggcgat ctgggtgccc
     2041 gcctgcggcg cgcgttcgcc gccgcgaccc ggcggggcgc gggtccgacg gtcgtcatcg
     2101 gcaccgacag cccgacgctg cccgagaccc acgtcttacg ggcgttcgag ctgctcgaga
     2161 gcggtgcccc ggcggtgatg gcaccgtctt acgacggcgg ctacgtgctg atcggctgcg
     2221 tcgggtcgcg atccgagctg ttcgagcggg tgccgtgggg cggtccggcg gtggcggaga
     2281 cgacccgtcg ccgggcggtc caggccggaa tcgccctgca tacgatcgat ccatggtacg
     2341 acgtcgacga ccgcgagggt ctcgaccgat tgcggcggga gcttcgcgac ccgtcggccg
     2401 cccgccgggc gccgcggacg gcccgcgcgt tgttggactt gccgttcgat cccgtggtat
     2461 agtcgttcgc cggtcaccgc caggcgaccg gcgacttacc ccgcttgaac cccgatctcg
     2521 gccgtgtcgg tcagatgtca ttccattcag gagccgcgcg ccgcgatgct ggagagactc
     2581 cggtgaatgc cgtcgcaccc aactcggcga gcgagtgcag gaggtcccat gtctgaagag
     2641 cgcccccgcg gacgccgcgg tccgacccga ggagaccgcc ggggaggacg ccgccgcggc
     2701 ccgtccgcac ccggaacgaa cctgcgcgaa gcggacgagg tcgcgctgcc cgaggccgag
     2761 accgagggca aagtcgccgt cgccgaagga ccgcccgacg acgacaacgt cgtcgacctc
     2821 aactcgctgt acaagatgga caaccacgcg ctggtcagcg cggccaagga cctgggcatc
     2881 ggcggctcga cctcgctgcg caagcaggag ctgattttcg agatcatgaa ggcgcagtcc
     2941 gagcggagcg ggctggtctt cgccgagggc gtactgcaga tcctgcagga cgggtacggc
     3001 ttcctgcgcc acccggacta caactacctg ccggggcccg acgacatcta cgtctcgcct
     3061 tcccagatca agcgcttcgg cttgaccacc ggcgacaccg tctcgggtca ggtgcggccg
     3121 cccaagcacg acgagaacta cttcgcgttg atcaaggtgc tcgccgtcaa cttcgacgac
     3181 ccggagcgaa tccgcgagcg gatcttcttc gacaacctca ccccactcta tccgctcgac
     3241 cacatccggc tcgagaccga cgccgagaac gtcgccggtc ggatcatgga tctgctcacg
     3301 cccatcggta agggccagcg cgggctgatc gtctcgccgc ctcgtaccgg caagacgatg
     3361 ctgctgcaat ctctcgccaa ctcgatcacg cgcaaccatc ccgaggcgtt cctgatcgtg
     3421 ctgttgatcg acgaacgtcc cgaggaagtg accgacatgc agcggtcggt tcagggcgag
     3481 gtcgtcgcct cgactttcga cgagcccgca caacagcacg tgcacgtcgc cgagatggtg
     3541 atcgaaaaag ccaagcggct cgtcgagcac ggcaaggatg tggtgatcct gctcgacagc
     3601 atcacccgac tggcgcgagc ctacaacacg gtgcaaccgc cgtcgggcaa ggtgctgtcg
     3661 ggcggcatcg acgccaacgc cctgcaacga cccaaacgct tcttcggcgc cgcgcgcaac
     3721 atcgaggagg ggggttcgct gacgatcatg gccacggcgc tgatcgatac cggttcgcgc
     3781 atggacgacg tgatcttcga ggagttcaag ggtacgggca acatggagat ccacctcgat
     3841 cgcaagctgg tcgaccggcg cgtcttcccg gcgatcgaca tcgagcggtc ggggacgcgc
     3901 aaggaagagc tgctgttggg caaggagacg ctgaacaagg tgtggatcct gcgcaagatc
     3961 ctcaaccaga tgtcggccgt cgaggcgatg gagctgttga tcgacaagct gaccaagagc
     4021 gagagcaacg ccgagttcct caagctgatg caaaatccga ccggctgagc agcggcgctc
     4081 aaccgacctg cgccagctcc agccgtctcc tccacttgcc acccagtccg acgagcatcc
     4141 gccgcgccgc ctcgcgcgac ggctcgccgg ccgcgaccag cgcgcgcgcc tcgcgccgcg
     4201 ccgcctcgag cagctccggg gtcttgacga cctgcgcgag gaactgcagg tcgctgagac
     4261 catgctgctg cgtgccgaag acgacgcccg ggccgcggat gtgcaggtcg cgttcggcga
     4321 tctggaatcc gtcgttcgtc cgctccatga cttccaaccg ctcgcgcgca tcctcgcccg
     4381 cctgctctcg gccgaccatc aacacgcagt acgaccgcgc cgcgccgcga ccgacgcggc
     4441 cgcgcagctg atggagctgc gacagaccga agcgctcggc gtgctcgatc agcatcaccg
     4501 tcgcgttcgg cacatcgacg ccgacctcga tcacggtggt cgagaccagg acttggatcc
     4561 ggccctcgcg aaactcctgc atgatccggt ccttgtcggc cggcttgagc cgaccgtgca
     4621 tcatgccgac ccgcagatcg gcgaacacct cggcggcgag atggtcggcg aactcggtcg
     4681 cggccttgag gtcgacgcgc tcgctctcgg caacgagcgg cacgacgacg taggcttgcc
     4741 gtccgcgttg cacctcccgg cgcaacccgt cgtagacctt ccgccgatcc gattccgcgc
     4801 ggacgagcgt gcgaatctcc ttgcggcccg gcggcagctc atcgatcacc gacaggtcga
     4861 gatcgccgta gagcgtcagc gccagcgagc gcgggatcgg cgtcgcggtc atcaccagca
     4921 cgtcgggatg ccggcccttg cgcgccaacg cggcgcgctg cagcacgccg aaccgatgtt
     4981 gctcgtcgat cacgaccagc ccgagtcgtc ggaagccgac accggcctcg aacagggcgt
     5041 gggttcccac cgccagctgg gcctcgcccg aggcgatccg ctcgaggacg cgcttcctct
     5101 ggggcgcgcg cagcgatccg gtcagcagct cgagccggca gtcgatctcg ctcggctcga
     5161 gcatcgatct caggttgcgg aagtgctgtt cggccaggat ctccgtcggc gccatcaacg
     5221 ccccctgata gccgttctcg atcgcgacca gcagggtcag cagcgccacc accgtcttgc
     5281 ccgatccgac gtcgccttgc aacaggcggt tcatcggatg atcggaggtg agatcggagc
     5341 cgatctcgcg cagcacgcgg cgctgggccg cggtcagttt gaacggcagc atgcgagcca
     5401 gccgcgtgcg aaacgcgtcc ggcaccgcgt aggcgatgcc gcgcccctgc tgctccaacc
     5461 cgtgccgccg cagcgccagc gccagttgca gcaggtagat ctcttcgaac gccagcgtac
     5521 gctggccggg ggtgcgtccg gccgcgagct cgtcgagcgc gacctcgtcg ggcgggaagt
     5581 ggatctcgcg caatgcccgc ctccgctcga tcaggccgtg ggcgcgtgcg acctcgagcg
     5641 gaatcagctc gcgcaggcct gccggcgcga gatcgctcaa cgcccggtgg atcgccgcac
     5701 gcagggcccg cgagctgaga tcggccagtt tgcggtagac cggtaccact cgtccggcgt
     5761 ggatgccctc gctgtcaccg ggctcgacga actcgtactc ggggttgtcg agcgtcgggc
     5821 ggcccgagcg gtcgatcgat gcgcgaccgt agagcacgac ctcgcgaccg gcggtcagca
     5881 ggcgttcgag gtacggctgg ttgtaccaga cggcgcggat cacgccgctc gcatcggcca
     5941 cggtcgcctc gaagatggtg aaaccgcggc gtcgcgtgcg gatcagccgc gaccgctcga
     6001 ccttgaccga caccgtgcac tcgcccgctt ccggccgtag ctcgcggacc gcggcgaacg
     6061 cccgacgatc ctcccagcgg aacggcagca cgtagagcag atcctcgacc gtctcgtatc
     6121 cgaggcggga gagcttctcg gcgcgccgcg gaccgacgcc cctgacgaaa cgcaactcgg
     6181 tgtcggcccg gatcgtgctc atcgcgcggt cgctataata ggccaccgtc cgcggccgac
     6241 ggcccgatgc cgtgctttcc gcgacgcgga ggattgcacc acggaaaccg ggccgctcgt
     6301 cgacgtcgtc atccccgcat tcaacgagag ccggtcggtc ggccgggtgc tcggcgacct
     6361 gccgcgcggc accctgcgcg aggtcgtggt cgtcgacaac gggtcgacgg accgcaccgc
     6421 cgaggtcgcc cgtgccgccg gcgcgaccgt cgtgagcgag aaccggcgcg gctacgggag
     6481 cgcctgcctg gccggtgtcg cccacctggc gcgcctgtcg cccgctccac agatcgtcgt
     6541 cttcgtcgac gccgactaca gcgatcacgc ggaagagctc cccgcgctga tcgcgccgat
     6601 cgtcgaagac cgtgccgaca tcgtcgtcgg ctcgc
//
LOCUS       DASPGN010000320        11270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_526120, whole genome shotgun sequence.
ACCESSION   DASPGN010000320 DASPGN010000000
VERSION     DASPGN010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11270
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526120"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VD788_09310"
     CDS             complement(<1..274)
                     /locus_tag="VD788_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV76504.1"
                     /translation="MSDGARIGLSMAVGILVGAVALGGAQDDKAAEAAVVPAKIPEEA
                     RLANNPRPATPESVDNGKLIFASQCTMCHGVTGKGDGDLAGRLGYAI"
     gene            413..826
                     /locus_tag="VD788_09315"
     CDS             413..826
                     /locus_tag="VD788_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXV76505.1"
                     /translation="MRRVRRSVTLGWLALAATAIVADERSIERGAAVYRIYCRGCHGD
                     GATGDGPTAERLTPHPTDLTRLAGDRGGTFPREPVMREIDGREKIPAHPRPIMPIWGL
                     AFQQFDTDVDQESEVRERLADLADYLESIQRRPPG"
     gene            complement(828..1499)
                     /locus_tag="VD788_09320"
     CDS             complement(828..1499)
                     /locus_tag="VD788_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HXV76506.1"
                     /translation="MGERRRVRFYFDFVSPYSWLALMTATEFAARHRVEWEMRPVVFA
                     ALLDAAGLVGPVESNVKRRYTFVDVWRCARRAGLRLVGPPAHPFRSIEALRAACVFQD
                     RPEALALVRRIAAACWSDGADLTDRRTLERLVRDTVAYDGPLATALDDPTIKRLLREN
                     TDRAIADGAFGVPTFVLDGRLFWGQDRMDQLADALAGRDDPEAAELAQLIDRPRGADR
                     RRTAG"
     gene            1642..3363
                     /locus_tag="VD788_09325"
     CDS             1642..3363
                     /locus_tag="VD788_09325"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV76507.1"
                     /translation="MLETVPEQEPAEGAAAIDRPAGAAPDLGPAIHPLVDGERAPGTG
                     RLIEVIDPSTGAPFARVACAGPEEIDAAAEAAARASALWRGSPYKQRGEALRRLAAII
                     YEDRLNLAELIAREQGKPFVEALALELLPALDHLKFITHRAEQYHGGLVVDPRHPYYT
                     HKQAHYLYDALGVVALITPSPLPFAIPLIQSAAALAMGNSVVLKPSERTPLSALRLGE
                     LCERAGFPAGVVNVVPAFPEDSLRLAAHPKIDKVFVTGGLVAGQSVMATAGCAPKPVV
                     LALGGKHPAIVAGDADVDRAARGIVWGALANCGQNCGSVERVYVEERVASRFLERVLS
                     EVDRLIVGESMDEGTDVGPMTTDERRREVHRQVGDAIKRGAKLVRGGVMPDGDGYFYP
                     ATVILDPPADCSLSRDETLGPVIPITVVESTERAILLANDCDYALTASGWTRSADCAG
                     RLMQGLHAGVVTINDLLYSFGEPAATWSGYRLSGMGQNHGTPGLREMSRQRFVSFDTT
                     VAEAPIFAFPYDETADRVVRSAIEHLHAGRRLQRLRAIGRLLRLKRFRRRVHTRSVLL
                     AKKRQVR"
     gene            3469..5010
                     /locus_tag="VD788_09330"
     CDS             3469..5010
                     /locus_tag="VD788_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HXV76508.1"
                     /translation="MKIPSLILRQLYTFGSLENSLAVVRFSLKNRLSDATLTGLTEVR
                     FDDDPVPLDLVSIDLGDGQTVAATAVSEHNPVVFPLRKTVEITAARAPLSSGPHRLKL
                     GFVAKPFGKLELSVQDSIADEKPPRVSVPYRKDNDYSTAAIAERQRFVERFSGIKPQH
                     LWQYSFDPQATRGNVENFTGVAQVPIGFAGPLRVNGEHAKGDFLVPLATSEGTLVASY
                     NRGIKLVNESGGVTCTVVSDRMQRAPVFVFDSAREARDFRGWVYQHFEEIRDAAEATS
                     RVAKLDDIDTYLSNRFAFLRFNYSTGDAAGQNMVGRATFAACSWILERAPEVRKFYLE
                     SNLATDKKASQINVMRTRGKRVVAEATIARDTLVQLMRVEPESLFHHYNVSNVGALLS
                     GANNNGCHSPNAITAMFIATGQDVANVSESSAGILYSELTPDKDLYISLTIPSLIVAT
                     HGGGTGLPTQRECLEIMGCTGRGTVNKLAEIVAGVALAGELSLASAISSLDWVSSHEQ
                     YGRNR"
     gene            5089..6120
                     /gene="nadA"
                     /locus_tag="VD788_09335"
     CDS             5089..6120
                     /gene="nadA"
                     /locus_tag="VD788_09335"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HXV76509.1"
                     /translation="MRTAEDLYTRMHERLARVVPEFELRHKAELAFEINRLKAERNAV
                     ILGHNYMEPALFHSVPDITGDSLELCRQAALTDKDPIVFCGVRFMAETAKILNPHRLV
                     LLPAREAGCSLAASIGAADVRALKRRFPGVPVVVYVNTYAEVKAEADYCCTSSNAARI
                     IDSLDSDTVIFLPDEYLARNVARETGKHIVFPSLLGGGDGQVDYQMIGWHGRCEVHDK
                     FTVDDVTNVRKQFPDAVILAHPECSPEVVVAADFSASTAAMIRYVEQSSAPHYLLLTE
                     CAMGDNIAAANPDKEMLRLCSVRCPHMNEITLEDTLAALQHDRYPIDIAEPIRARAER
                     SIRRMLEIG"
     gene            6131..7768
                     /gene="nadB"
                     /locus_tag="VD788_09340"
     CDS             6131..7768
                     /gene="nadB"
                     /locus_tag="VD788_09340"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HXV76510.1"
                     /translation="MRTDTLIIGSGIAGATAALRLADNPGHRVTVITREADPTESNSD
                     WAQGGIVGRGANDTAELLAEDILRTGAGLSSPPAVRLLAEEGPRLLRELLIDAGGPSF
                     DRDESGEPAFGLEGAHSRRRILHVGDSTGHAIMTRLIERLAERPQIELLTGRTAVDLI
                     TYPHHARDPLRVYDEPTCQGAYVLDTAAGVVEPILARQTILATGGLGQIFLNSSNPPG
                     TRGDGLAMAHRAGARVVNAEYVQFHPTTLHLPGPTKFLISEAVRGEGARLLTPQGEPF
                     MERYSARWKDLAPRDIVARAIYWEMLEHDLPHVMLDLASHRPAEFIRERFPQIYERCK
                     ARGLDITQQPIPVVPAAHYFCGGVLVDLWGRTTLPRLYAVGEVACTGVHGANRLASTS
                     LLEGLVWGARAAQSIDESAREPAVDETAVPPWDVSDLRFDPDPALVQGDMQTIRTLMW
                     HYVGLVRNEHRLTRAIRELTHLALSIEDFYRRARLTDGLIGLRNAIQVALLVARSAHH
                     NRTSRGCHYREDTRAEAPDPPAPSGGRRSAVEVPPGG"
     gene            complement(7773..9140)
                     /locus_tag="VD788_09345"
     CDS             complement(7773..9140)
                     /locus_tag="VD788_09345"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HXV76511.1"
                     /translation="MKTIFEPFRVKSVERLRPTTRDERREILARADYNLFRVSADDIM
                     IDLLTDSGTGAMSAEQWAGMMRGDESYAGARSWFRFRDRVRELTGFRHIVPTHQGRAA
                     ERILFSCLDVAGKTVVSNTHFDTTRANVEYLGGRAVDIPIAAALRPSEFHPFKGDIDL
                     DALVGLLDGPDEIACVVHTVTNNSGGGQPASMGNLRAASRACRQRRVPFFLDACRFAE
                     NAYFIKLREAGFEHREVESIAREMFSLADGATFSAKKDGLANIGGFLASNDDRLARQE
                     VELLILTEGFPTYGGLAGRDLEAIAIGLGEVVDEEYLRYRIASTRYLGDGLTELGYPI
                     IQPPGGHAIYIDAGSFLPRIPRDRFPGQALACAFYVEGGVRSVEIGSLMFGGEDPKTH
                     QHRHAALELLRLAIPRRVYTQSHMDYVLEVGAEIVERRSELAGYRIVEESPQLRHFTA
                     RLAPI"
     gene            complement(9176..10414)
                     /locus_tag="VD788_09350"
     CDS             complement(9176..10414)
                     /locus_tag="VD788_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXV76512.1"
                     /translation="MRKIRSDRGRIGLALAGGGFEGAVYEIGALYALSDSLDGFDLNR
                     DVDIFVGVSAGAFVASCLANGLTTRNLVRAMLRQSGVDHPFAPEVLFSPAIGEIVNRT
                     LMTPRLLVEAMLDYVRNPEDLSLLESLTRLSRALPVAMFDNEPIRAYLEGIFSLPGRT
                     DDFRQLKRRLVVVATDLDSGEAVRFGAPGADGVPISSAVQASTALPGLYPPVLIDGRH
                     YVDGVLLKTLHASVALEANAELVFCINPIVPVDTAEAVNAGAMKRGKLIHRGLPTVMS
                     QTFRTLVHSRLSVGLASYEPRFPGQHVVLFEPPRDDYRMFFTNVFRFSSRKRICEHAF
                     DATRRQLRERRETLEPLLAGSGITYRNDRLADPARDLWGTVGLGATARATRVTAELGS
                     LLDRLESILERLEAKSVIPG"
     gene            complement(10431..10856)
                     /locus_tag="VD788_09355"
     CDS             complement(10431..10856)
                     /locus_tag="VD788_09355"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXV76513.1"
                     /translation="MATKKKSDALPSGGTAHRVWLAGLGALASAEAEGGKLFERLVER
                     GREFSGQVGKPLEEATDRVRETMAGVRGRAGRTLKKVEGAFDQQLTAALKRLGVPTRT
                     EVIDLTQRVEALNRKLDAAAQARPNEKKTGKKVTRKKKA"
     gene            complement(10873..>11270)
                     /locus_tag="VD788_09360"
     CDS             complement(10873..>11270)
                     /locus_tag="VD788_09360"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXV76514.1"
                     /translation="GIAKEIEMNKKVTKATDQAKKFGDEVFKVGRNFWLAGLGVYSLA
                     EEETRKAVDQLIKRGEEFEKDDKNVVSKTIDQATDRAKRFGNEVEDRVQEAVGGMLHR
                     AGIPSRAEIRTLIDRVEELNSKVEKLRAH"
BASE COUNT         1832 a         3865 c         3786 g         1787 t
ORIGIN      
        1 ggatcgcgta acccagacgg ccggccagat caccgtcgcc cttgccggtg accccgtggc
       61 acatcgtgca ctgactggca aagatcagct tgccgttgtc gaccgattcc ggtgtcgccg
      121 gacgagggtt gttggcgagc cgcgcctcct cggggatctt cgccgggacg accgccgcct
      181 ccgccgcctt gtcgtcctgg gccccaccga gcgccaccgc gccgacgaga atccccaccg
      241 ccatcgacaa tccgattcgc gcaccgtcgc tcatcggccg ttcctccggc tgggtcgaaa
      301 cccaagaagc agcttaccac cgggcggcgg cgcgccgcgc gggcggcaac cggaccgacc
      361 gggtcccgag gggcgggggc gggacgccct gcccggatgg tacggttgag gcgtgagacg
      421 ggttcgacgg tcggtgacgc tgggatggct cgccctcgcc gcgaccgcga tcgtcgccga
      481 cgagcggtcg atcgagcggg gcgcggcggt ctaccggatc tactgccgtg gctgccacgg
      541 cgacggtgcc acgggtgacg gcccgacggc ggagcggctc acgccccacc ccaccgacct
      601 gacgagactg gccggggatc gtggcgggac gttcccgcgc gagccggtga tgcgggagat
      661 cgacgggcgc gagaagatcc cggcccatcc gcggccgatc atgccgatct ggggtctggc
      721 cttccagcag ttcgacaccg acgtcgatca ggaatcggag gttcgggagc gcctcgccga
      781 tctcgccgat tacctcgagt cgatccagcg acgcccaccg ggttgactca gcccgccgtc
      841 cgccgacggt cggcgccgcg agggcgatcg atcagttgcg ccaactcggc cgcctcgggg
      901 tcgtcgcgac cggcgagtgc atcagcgagc tggtccatcc ggtcctgtcc ccagaacagc
      961 cggccgtcga gcacgaaggt cggcacgccg aacgccccgt cggcgatcgc tcgatcggtg
     1021 ttctcgcgca acagccgttt gatcgttgga tcgtcgagcg cggtggcgag cggcccgtcg
     1081 tacgcgaccg tgtcgcgcac caggcgctcg agcgtgcgac ggtcggtcag gtccgcgccg
     1141 tcgctccagc aggcggcggc gatccgccgg accagcgcca gcgcctcggg ccggtcctgg
     1201 aagacgcagg ccgcgcgaag cgcttcgatc gagcggaacg ggtgagccgg aggaccgacg
     1261 aggcgcagcc cggctcggcg ggcgcaacgc cagacgtcga cgaaggtgta gcggcgtttg
     1321 acattcgatt cgaccggtcc gaccagtccc gccgcgtcga gcagcgccgc gaaaacgacc
     1381 gggcgcatct cccactcgac ccggtgtcgg gccgcgaact ccgtagccgt catcagcgcg
     1441 agccaggagt acggagagac gaagtcgaaa taaaagcgga ctcggcgacg ttcacccaca
     1501 aatggctcct cgatcggtcg aacggccgcg gcccggaacc accaccctat ctgtttgatt
     1561 ctagtgggtt ttgccagagg cttccgcgcg aatcgcgtcg cagacctgcc gcggggcccg
     1621 ccggagcata tattcggccc catgttggag accgtccccg aacaggagcc ggcggagggc
     1681 gcagctgcga tcgatcgtcc agcgggggcc gcgcccgacc tcggaccggc gatccatccg
     1741 ctcgtcgacg gggagcgggc gcccggcacc gggcgcctga tcgaggtgat cgacccctcg
     1801 accggcgcgc cgttcgcgcg cgtcgcgtgc gccggccccg aggagatcga cgcggcggcc
     1861 gaggccgccg cccgcgcatc ggcgctgtgg cgcggctctc cgtacaaaca gcgcggtgag
     1921 gcactgcgcc ggctggccgc gatcatctac gaggatcggc tcaacctggc cgagctgatc
     1981 gcgcgcgagc agggcaagcc gttcgtcgaa gcgctcgcgc tcgagctatt gccggcgctc
     2041 gaccatctga agttcatcac gcaccgggcc gagcagtacc acgggggtct ggtcgtcgac
     2101 ccccggcacc cgtactacac ccacaaacag gcgcactacc tgtacgacgc cctcggcgtc
     2161 gtcgcgctga tcacgccgtc accgctgccg ttcgcgatcc cgttgatcca gtcggcagcg
     2221 gcgctcgcga tgggaaactc ggtggtgctc aagccgtccg agcgcacgcc gctgtcggcg
     2281 ctgcgactcg gggagctgtg cgagcgcgcg gggtttcccg ccggcgtcgt caacgtcgtc
     2341 ccggcgttcc ccgaggactc gttgcgtctc gccgcacacc ccaagatcga caaggtgttc
     2401 gtcaccggtg ggctcgtcgc cgggcaaagc gtgatggcga ccgcgggctg cgcgccgaag
     2461 ccggtcgtgc tggccctcgg cggcaagcac ccggcgatcg tcgcgggcga cgccgacgtc
     2521 gatcgcgccg cgcgcgggat cgtctggggc gcgttggcca actgcgggca gaactgcggc
     2581 tcggtcgagc gtgtctacgt cgaggagcgt gtcgcatcgc ggtttctcga gcgggtgttg
     2641 tccgaagtgg accggctgat cgtcggcgaa tcgatggacg aggggaccga cgtcgggccg
     2701 atgacgaccg acgagcggcg tcgcgaggtc catcggcagg tgggcgatgc gatcaagcgc
     2761 ggggccaagc tcgtgcgcgg cggcgtgatg ccggacggcg acgggtactt ctatcccgcg
     2821 acggtgattc tcgatccgcc ggcggattgc agcctcagcc gcgacgagac gctcggcccg
     2881 gtcatcccga tcacggtcgt cgagagcacc gaacgcgcga tcttgctggc caacgactgc
     2941 gactacgcgt tgaccgccag cgggtggacg cggtcggccg attgcgcggg tcgactgatg
     3001 cagggtctgc acgcgggtgt cgtcacgatc aatgacctgc tctactcgtt cggcgagccg
     3061 gccgccacct ggtccggcta ccgcttgagt ggcatgggtc agaaccacgg aacccccggc
     3121 ttgcgcgaga tgtcgcggca acggttcgtt tcgttcgata cgacggtggc cgaggcgccg
     3181 atcttcgcct ttccctacga cgagaccgcc gaccgcgtcg tccgctccgc gatcgagcac
     3241 ctgcacgccg gtcgacgtct ccagcgactg cgtgcgatcg ggcggttgct gcggctgaag
     3301 cgcttccgcc gccgcgtcca cacgcgcagc gtgctgctcg cgaaaaaacg ccaggtgcgt
     3361 tgaccgcccg cgatctttcc gcacgacgat cggtggcata atggaggcga gccgacttcg
     3421 gttcgcttcc acgggtgcat tccgcgacat cgctgaaagg gccggtcgat gaagatcccc
     3481 tcgttgattc tccggcagct ctataccttc ggcagtctcg agaattcgct cgcggtcgtc
     3541 cgcttctcgc tgaagaaccg cctgagcgac gccacgctga ccggcctgac cgaggtgcgg
     3601 ttcgacgacg atccggtccc gctcgatctc gtttcgatcg atctcggtga cggccagacc
     3661 gttgccgcca ccgcggtcag cgagcacaac ccggtcgtct ttccgttgcg caagaccgtc
     3721 gagatcaccg ccgcgcgggc accgctgtcg agcggcccgc accgactcaa actgggcttc
     3781 gtggcaaaac ccttcggcaa gttggaactg agcgtccagg attcgatcgc cgacgagaag
     3841 ccgccacgcg tcagcgtgcc gtaccgcaag gacaacgact actcgaccgc cgcgatcgcc
     3901 gagcgccagc gcttcgtcga gcgattctcc ggaatcaagc cgcagcacct gtggcagtac
     3961 tcgttcgacc cgcaggcgac acgcggcaac gtcgagaact tcaccggcgt ggcgcaggta
     4021 ccgatcggct tcgccgggcc gctgcgcgtc aacggggagc acgcgaaagg cgatttcctc
     4081 gtcccgttgg cgacgagcga gggcacgctc gtcgcctcgt acaaccgcgg gatcaagctg
     4141 gtcaacgagt ccggcggggt gacgtgcacg gtggtcagcg accgcatgca gcgcgcgccg
     4201 gtgttcgtct tcgacagcgc gcgcgaggcg cgggattttc gcggctgggt ctaccagcac
     4261 ttcgaagaga ttcgcgacgc ggccgaggcg acgtcgcggg tggccaagct cgacgacatc
     4321 gacacctacc tgtccaaccg cttcgcgttc ctacgcttca attactcgac cggcgatgcg
     4381 gcgggacaga acatggtcgg acgcgcgacg ttcgccgcct gcagctggat actcgaacgg
     4441 gcccccgagg tgcgcaagtt ctatctcgag tcgaacctgg cgaccgacaa gaaggcatct
     4501 cagatcaacg tgatgcgcac ccgcggcaag cgggtggtgg ccgaggcgac gattgcgcgg
     4561 gacacgctcg tgcagttgat gcgcgtcgag ccggagagcc tgttccatca ctacaacgtt
     4621 tcgaacgtcg gcgcgctgct gtccggcgcc aacaacaacg gatgccattc cccgaacgcc
     4681 atcaccgcga tgttcatcgc gaccggccag gacgtggcca acgtgtcgga atcgtccgcc
     4741 gggatcctct attccgagct gacccccgac aaggacttgt acatctcgct gacgatcccg
     4801 tcgctgatcg tcgcgacgca cggcggtggc accggtctgc cgacccagcg cgagtgcctc
     4861 gagatcatgg gttgcacggg acgcgggacg gtcaacaagc tcgcggagat cgtcgccggt
     4921 gtcgcgctgg ccggagaatt gtcgctggcg tccgccattt cttcgctcga ctgggtgtcg
     4981 agccacgagc agtacgggcg caatcgatga cggcccgccg ccaccgtccg gggaggcgga
     5041 cgcgcggcgg tcgtgggata ctggtcagcg cggagaggat ctgaatcgat gcggaccgcc
     5101 gaagacctgt acacgcggat gcacgagcgg ttggcccgag tggttcccga gttcgaactg
     5161 cgccacaagg ccgaactggc gttcgagatc aaccggctca aggccgaacg aaacgcggtg
     5221 attctcggcc acaactacat ggagccggcg ctgttccact cggtgcccga catcaccggc
     5281 gattcgctcg aattgtgtcg tcaggccgcg ttgaccgaca aagacccgat cgtcttctgc
     5341 ggcgtacgtt tcatggcgga gacggcgaag atcctcaatc cgcacagatt ggtgctgctg
     5401 cccgctcgcg aggccggctg ctccttggcc gccagcatcg gcgcggccga cgtccgtgcg
     5461 ctgaagcgga ggttcccggg cgttccggtg gtggtctacg tcaacacgta cgccgaggta
     5521 aaggccgagg cggactactg ctgcacgtcg agcaacgcgg cccgcatcat cgactcgctc
     5581 gacagcgaca cggtgatctt cttgcccgat gagtacctcg cccgcaacgt ggcgcgggag
     5641 accggcaagc acatcgtctt ccccagcctg ctgggcggcg gcgacggaca agtcgactat
     5701 cagatgatcg gctggcacgg acggtgcgag gtgcacgaca agttcaccgt cgatgacgtg
     5761 accaacgtcc gcaagcagtt tccggacgcg gtcatcctgg cgcatcccga gtgcagcccg
     5821 gaagtagtcg tcgccgccga tttctcggcg agcaccgcgg cgatgattcg atacgtcgag
     5881 cagtcgagcg caccacacta cctgctgctc accgagtgtg cgatgggtga caacatcgcg
     5941 gcggccaacc ccgacaagga gatgctgcgc ctatgcagcg tccgttgccc gcacatgaac
     6001 gagatcacgc tcgaagacac gttggctgcg ctgcagcacg atcgctatcc gatcgatatc
     6061 gccgagccga tccgagcgcg tgccgagcgc tcgattcggc gcatgttgga gatcggctga
     6121 tcaccggacg atgcgaaccg acacgctgat catcggcagt ggaatcgcgg gggcgaccgc
     6181 cgccctgcgt ctggccgaca acccggggca ccgcgtgacg gtcatcaccc gcgaggccga
     6241 cccgaccgag tcgaactccg actgggcgca gggcggaatc gtcggccgcg gcgcgaacga
     6301 cacggcggaa ctgctggcgg aggacatcct gcgcaccggg gccggtctct cctccccgcc
     6361 ggccgtgcgg ctgctggccg aagagggtcc gcggctgctt cgcgaactgt tgatcgacgc
     6421 cggcggcccg tcgttcgacc gagacgagtc gggcgagccg gcattcggct tggaaggggc
     6481 acactcgcga cgccggattc tccacgtcgg cgacagcacc ggacacgcga tcatgacgcg
     6541 gttgatcgaa cgcctcgccg aacgaccgca gatcgagctg ctgacgggca gaacggcggt
     6601 cgacctgatc acctatccgc accacgcgcg cgacccgcta cgcgtctacg acgagccgac
     6661 gtgccaaggt gcctacgtgc tcgacacggc ggcaggcgtg gtcgagccga ttctggcgcg
     6721 ccagacgatt ctggccaccg gcggactagg acagatcttc ctcaatagca gcaaccctcc
     6781 cgggacccgc ggcgacgggc tggcgatggc ccatcgcgcg ggagcgcggg tggtcaacgc
     6841 cgagtacgtg cagttccacc ccacgaccct gcacctaccg ggcccgacca agttcctgat
     6901 cagcgaggcg gttcgcggcg agggcgcccg gctgctcacg ccgcaaggcg agccgttcat
     6961 ggagcgttac tccgcgaggt ggaaggacct ggcaccgagg gacatcgtgg cgcgggcgat
     7021 ctactgggag atgctcgagc acgacctgcc ccacgtgatg ctcgacctgg cgtcgcatcg
     7081 cccggcggag ttcatccgtg agcgattccc gcagatctac gagcgctgca aggcccgggg
     7141 tctcgacatc acgcagcaac cgatcccggt cgtccccgcc gcacattact tctgtggcgg
     7201 cgtgctggtc gatctgtggg gccggacgac ccttccgcgg ctgtatgcgg tgggggaggt
     7261 cgcctgcacc ggcgtgcacg gcgcgaaccg actggcgagc acgtcgctgc tcgaagggct
     7321 ggtttggggc gcgcgtgccg cgcagtcgat cgacgagagc gcgcgcgaac cggcagtcga
     7381 cgaaacggcc gtgccgccgt gggacgtcag cgatctgcgc ttcgatccgg accccgcgct
     7441 ggtgcagggc gacatgcaga cgatccgcac gctgatgtgg cactacgtcg gcctggtgcg
     7501 caacgagcac cgtctgactc gggcgatccg cgaactgacc cacctcgcgc tgtcgatcga
     7561 ggacttttac cggcgggcgc ggttgaccga cgggttgatc ggtctgcgca acgcgattca
     7621 agtggcgctg ctcgtcgctc gttcggcgca ccacaatcgt acgagccgcg gctgccacta
     7681 ccgcgaggac acgcgggcgg aggcgcccga tccgccggcg ccgtccggcg gccggcggtc
     7741 tgcggtcgag gtgccgccgg gcggctgaag gatcagatcg gcgccaagcg cgcggtgaag
     7801 tgtcgcagct gcggcgactc ctcgacgatg cggtagcccg ccagctccga gcgccgctcg
     7861 acgatctccg cgccgacctc gagcacgtag tccatgtggc tctgcgtgta gacccgacgc
     7921 gggatcgcca gccggagcag ttcgagcgcg gcgtggcggt gttggtgcgt cttgggatcc
     7981 tcgccaccga acatcaggct gccgatctcg acggagcgca cgccaccctc gacgtaaaac
     8041 gcgcacgcca acgcctgtcc cgggaaacga tcgcgcggga tccggggcaa gaaactcccg
     8101 gcgtcgatgt agatcgcatg acccccgggc ggctggatga tcgggtagcc gagctcggtc
     8161 agcccgtcgc ccaggtatcg cgtcgatgcg atgcggtagc gcaggtattc ctcgtcgacg
     8221 acctcgccaa gcccgatcgc gatcgcctcg agatcgcgcc cggcgagacc gccgtaggtc
     8281 gggaacccct ccgtcaggat caacagctcg acctcctgac gggccagacg gtcgtcgttg
     8341 ctcgcgagga agccgccgat gttggccaag ccgtctttct tggcgctgaa ggtggcgccg
     8401 tcggccaacg agaacatctc gcgggcgatc gactcgacct ctcggtgctc gaagccggcc
     8461 tcgcgcagct tgatgaagta ggcgttctcg gcgaagcggc aggcgtcgag gaagaacggc
     8521 actcggcgct ggcgacacgc gcgactcgcg gctcgcagat tgcccatcga tgccggctgc
     8581 ccgccgcccg agttgttggt gacggtgtgc acgacgcagg cgatctcgtc cggcccgtcg
     8641 agcagtccga ccagcgcgtc gaggtcgatg tcgcccttga acgggtgaaa ctccgacgga
     8701 cggagcgcgg cggcgatcgg aatgtcgacc gcccgtccgc cgaggtattc gacgttggcg
     8761 cgcgtcgtgt cgaagtgggt gttgctgacg accgtcttgc cggcaacgtc caggcaggaa
     8821 aacaagatgc gttccgccgc gcggccctga tgggtcggga cgatgtgacg aaacccggtc
     8881 aactcccgga cgcggtcgcg aaaacgaaac cacgagcgcg ctccggcgta ggattcgtcg
     8941 ccgcgcatca tcccggccca ctgctcggcc gacatcgcgc ccgtgcccga gtcggtcaag
     9001 aggtcgatca tgatgtcgtc ggcgcttacc cggaacaggt tgtagtcggc ccgggccagg
     9061 atctcgcgac gttcgtcgcg ggtcgtcggg cgcagtcgct cgaccgactt gacgcggaac
     9121 ggttcgaaga tcgtcttcat ccgtgaagcc cccccgggcg actccgggtc gcacgctagc
     9181 cgggaatgac cgacttggcc tcgagccgct cgagtatcga ctccagtcgg tcgagcagcg
     9241 acccgagctc ggccgtcaca cgggtcgccc gcgccgtcgc gccgagaccg acggtgcccc
     9301 acagatcgcg cgccggatcg gccagacggt cgtttcggta ggtgatcccg ctcccggcca
     9361 gcagcggctc gagcgtctcg cgccgttcgc gcaactggcg gcgggtcgcg tcgaacgcgt
     9421 gctcgcagat cctcttgcgc gacgaaaagc ggaagacgtt ggtaaagaac atccggtagt
     9481 cgtcccgcgg tggttcgaac aacacgacgt gctgaccggg aaaccgcggt tcgtacgacg
     9541 ccagtcccac gctcagccgc gagtggacca gcgtgcggaa ggtctgggac atcacggtcg
     9601 gcaacccgcg gtggatcaac ttgccgcgtt tcatcgcccc ggcattgacc gcctcggcgg
     9661 tgtcgaccgg cacgatcggg ttgatgcaga acaccagctc cgcgttggcc tcgagtgcga
     9721 ccgaggcgtg gagcgtcttg agcagcacgc catcgacgta atgacggcca tcgatcaaca
     9781 ccggcggata gagccccggc agcgcggtgc tggcctgcac cgcactcgag atcggcactc
     9841 cgtcggcacc cggcgccccg aagcgcacgg cctctcccga atcgaggtcg gtggcgacga
     9901 cgaccagcct ccgcttcagc tgccggaagt cgtcggtgcg ccccggcagc gagaagatac
     9961 cctcgaggta ggcccggatc ggttcgttgt cgaacatcgc gaccggcagg gcgcgcgaca
    10021 ggcgcgtcag cgattcgagc aggctgaggt cctcggggtt gcgaacgtaa tcgagcatcg
    10081 cctcgacgag caggcggggc gtcatcagcg tgcggttgac gatctcgccg atggcgggag
    10141 agaaaaggac ctcgggagcg aaggggtgat ccacgccgga ttgcctgagc atcgcgcgaa
    10201 cgaggttccg tgtcgtcagg ccgttcgcca ggcacgatgc gacgaacgcg ccggcgctca
    10261 cgccgacgaa gatgtcgacg tcgcgattga ggtcgaaccc gtccaacgag tccgacaggg
    10321 cgtacagcgc gccgatctcg tagacggcgc cctcgaaacc tccaccggcg agcgccagcc
    10381 cgatgcgtcc ccggtcgctg cggatcttac gcataccacg ccccggagaa tcaggccttc
    10441 ttctttcgcg tcaccttctt cccggtcttt ttctcgttcg ggcgcgcctg ggccgccgcg
    10501 tcgagcttgc gattgagcgc ctcgacgcgc tgcgtcaggt cgatcacctc ggtccgcgtc
    10561 gggacgccga gccgcttcag cgccgcggtc agctgttggt cgaacgcgcc ctcgaccttc
    10621 ttcagcgtcc gtccggcccg cccgcgaacc ccggccatcg tctcgcgcac gcgatcggtg
    10681 gcttcttcca gcggcttgcc cacctggccg ctgaactccc gaccgcgctc gaccaggcgc
    10741 tcgaacagct tgcctccctc cgcctcggcg ctcgccagcg cacccagacc ggcgagccat
    10801 acacggtgcg ccgtaccgcc cgacggcagg gcgtcggact tcttcttggt tgccatcgac
    10861 cgacctcgcg ggctagtgcg ccctcagctt ttcgaccttg ctgttcagtt cctcgacgcg
    10921 atcgatcagc gttcggatct cggctcgcga cgggatgccg gcacgatgca gcataccgcc
    10981 gaccgcctcc tgcacccggt cctcgacctc gttgccgaac cgcttggcac gatcggtcgc
    11041 ctgatcgatc gtcttgctca cgacgttctt gtcgtccttc tcgaactcct cgccgcgctt
    11101 gatcagctgg tccaccgcct tgcgcgtctc ctcctcggcc agcgagtaga cgccgagacc
    11161 ggcgagccag aagttgcgac cgactttgaa cacctcgtcg ccgaacttct tcgcttgatc
    11221 cgttgccttc gtgaccttct tgttcatctc gatctccttg gctatccctg
//
LOCUS       DASPGN010000321         3300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1228530, whole genome shotgun sequence.
ACCESSION   DASPGN010000321 DASPGN010000000
VERSION     DASPGN010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228530"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2405
                     /locus_tag="VD788_09365"
     CDS             <1..2405
                     /locus_tag="VD788_09365"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV76515.1"
                     /translation="LIVFYAASEIVVTLVPARRFGGRWFRVGLGAADLVLVALGIQLA
                     GHVDRTLWASVFLMILIVALGNYRAHLVAGAAAVGAFHAWLVLGRDHGPEGLWDLALQ
                     ILFLSTMAMYYGYCVRDIHLVRRRREAQQLQNDELRVLLEILEAINSSLDLHRVCLTI
                     VQKLTAIIPAQRCSMLFVDAEAQRCYVIASHDDPSLTMLEIDLRKYPEVRRSIETRNA
                     VLVQNAPDDPVMAEVKEMLKGLDFRSILVVPLTFGSEVLGTLCLKTARGREDYSEDEL
                     RFCTAVARASAHALKNALLHDQVQRESGRYRTAMEKLSRILEQSPDLILTTDNDGAIT
                     EFNRGAERLLDRSREQVTGQHLRDLFPSCPTDLIERLRVDGKLSNQACRFLRPDGSEL
                     TLELNASALVDEHGEVRGAVWVGRDVTALKSAQTQLLQAKKLSSIGEVISGVAHELNN
                     PLSGVLGYSQLLVARNPNSPLARDLEKIYESALRCQKIVKNLLSFARVHKPERKYLGV
                     NGIIDKTLELRRYQLQVNDIEVVRRFDPDLPRTMLDFHQMQQVFLNLITNAQHAMTAV
                     RGRPGRLVVSTRQVHGRIEIRFADNGVGMDGDTLERAFDPFFTTKELGRGTGLGLSVS
                     YGIIKEHGGSIYANGRPGEGATFVIELPIREGQEDELASGQGLTERTEIVAADAGGRL
                     LVVDDEPMILDLFVDLFEDQDLLVDTAASGTEACRKVKQRNYDVVITDVRMPQMNGFE
                     LYSEIMSIRPEMEGRVIFMTGDLIDENTVRFLSQINARSIAKPLEIPAVLAAVSETLG
                     VD"
     gene            2489..>3300
                     /locus_tag="VD788_09370"
     CDS             2489..>3300
                     /locus_tag="VD788_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76516.1"
                     /translation="MSTNPAAGDLFGGPLALSIAGAVWVWLLAWMLGAALFPLGHRLH
                     DRSRLLAWAVIGIAVEAQLWLVLGWFGKLWPPIVVGVGVGITVGAALVAVPRIARELR
                     VEGGRAVTPGRSWLAVTACAALVLFVALVLRLALWPTVFYDDLVYHLGVPRQALLTGT
                     WPAMQGLHYSFMPAGWDATYVLPLAFGAGSAPQLMNVIALVSFAWAVYRLARFGGGPD
                     GAAVATALLVIAPVTGSSGAFAGNDLFVALALAVAAERLVATAASRPRLVGL"
BASE COUNT          490 a         1119 c         1130 g          561 t
ORIGIN      
        1 cgctgatcgt gttctatgcc gcttccgaga tcgtggtgac cctggttccc gcccggcgct
       61 tcggcgggcg ctggttccgc gtcgggctcg gcgccgccga cctggtgctg gtcgcgctcg
      121 gcatccagct cgccggccac gtcgaccgga cgctgtgggc ctccgtcttc ctgatgatcc
      181 tgatcgtcgc gctgggcaac taccgggcgc acctggtcgc gggcgccgcc gccgtcggcg
      241 cgttccatgc ctggttggtg ctgggacgcg atcacggccc ggagggtctg tgggacctcg
      301 ccctgcagat cctgttcctg tcgacgatgg cgatgtacta cggctactgc gtccgtgaca
      361 tccacctggt ccggcgccgc cgcgaggcgc agcagctgca gaacgacgag ctgcgcgtgc
      421 tgctcgagat cctcgaagcg atcaactcct cgctcgactt acaccgagtc tgtctgacga
      481 tcgtgcagaa gctgaccgcg atcattccgg cgcagcgctg ctcgatgctg ttcgtcgacg
      541 ccgaggcgca acggtgttac gtcatcgcct cgcacgacga cccgtcgctg acgatgctcg
      601 agatcgactt gcggaagtat ccggaagtcc gtcgctcgat cgagacgcgc aacgccgtgc
      661 tggtccagaa cgccccggac gacccggtga tggccgaagt caaggagatg ctcaaggggc
      721 tcgacttccg ttcgatcctc gtcgtgccgc tgaccttcgg ctccgaggtg ctcggcacgt
      781 tgtgtctgaa gaccgcccgc gggcgcgagg actacagcga ggacgagctt cggttctgca
      841 ccgcagtcgc acgcgcgtcg gcccacgcat tgaagaacgc gctgctgcac gaccaggtcc
      901 aacgggaatc cgggcgctac cgcacggcga tggagaagct gtcgcgcatc ctcgagcagt
      961 cgccggacct gatcctgacg accgacaacg acggcgcgat caccgagttc aaccgcggcg
     1021 ccgagcggct gctcgaccgc tcgcgcgaac aggtgaccgg tcagcacctg cgtgacctgt
     1081 tcccgtcgtg tccgacggac ctgatcgaac ggctgcgcgt cgacggcaag ctgagcaacc
     1141 aagcctgccg gtttctccgg cccgacggct cggagctgac gctcgagctg aacgcctccg
     1201 cgctggtcga cgagcacggc gaggtccgcg gcgcggtgtg ggtcggccgg gacgtcaccg
     1261 cgctcaagtc ggcccagacg cagctgcttc aggccaagaa gctgtcgagc atcggcgagg
     1321 tgatctcggg cgtggcccac gagctcaaca atccgctgag cggcgtgctg ggctactcgc
     1381 aactgctcgt cgcgcgcaac ccgaacagcc cgctggcgcg cgatctggag aagatctacg
     1441 agtcggcgct gcgctgccag aagatcgtga agaacctgct gtcgttcgcc cgggtccaca
     1501 agcccgagcg gaagtacctc ggcgtcaacg gcatcatcga caagacgctc gagctgcgtc
     1561 gctaccagct ccaggtgaac gacatcgaag tcgtacggcg gttcgacccc gacctgccgc
     1621 ggacgatgct cgactttcat cagatgcagc aggtgttcct caatctgatc accaacgcgc
     1681 agcacgcaat gaccgcggtg cggggtcgcc ccggccggtt ggtcgtctcg acgcgtcagg
     1741 tccacggcag gatcgagatt cgtttcgccg acaacggcgt gggaatggac ggcgacacgc
     1801 tcgagcgcgc attcgatccg ttcttcacca ccaaggagct gggccgcggg acagggctcg
     1861 ggctgtcggt ctcgtacggc atcatcaagg agcacggcgg gtcgatctac gccaacggcc
     1921 gccccggcga gggggcgacc ttcgtcatcg agctgccgat ccgcgaggga caggaggacg
     1981 agctggccag cggccaaggc ctcaccgaac ggaccgagat cgtcgcggcc gacgcgggag
     2041 gccggctgtt ggtcgtcgac gacgagccga tgatcctcga cctgttcgtc gacctgttcg
     2101 aagaccagga tctgctcgtc gacaccgccg ccagcgggac cgaagcctgc cgcaaggtca
     2161 agcagcgcaa ctacgacgtc gtgatcaccg acgtgcgcat gccgcagatg aacgggttcg
     2221 agctctactc cgagatcatg tcgattcgcc ccgagatgga aggtcgtgtc atcttcatga
     2281 ccggcgacct gatcgacgag aataccgtcc gcttcctctc gcagatcaac gcgcgcagca
     2341 tcgccaagcc gctcgagatc ccggcggtgc tggcggcggt atccgagacc ctgggcgtcg
     2401 actgaccgag cgcttgaccg ccggaaagct ttgaatcacg ttacgcggtg ggctggcgga
     2461 gctcgcccgc cacgcgaggg gcaatcccgt gtcgaccaac ccggcagcag gcgatctgtt
     2521 cggcggaccg ctcgcgctgt cgatcgccgg cgccgtctgg gtttggctgt tggcgtggat
     2581 gctgggagcg gcgctgtttc cgctcggtca ccggctgcac gaccgatccc gcctgctcgc
     2641 ctgggccgtg atcgggatcg ccgtcgaggc gcagctatgg ctggtgctcg gctggttcgg
     2701 caagctgtgg ccgccgatcg tcgtcggcgt cggcgtcggg atcacggtcg gagcggcgct
     2761 cgtcgccgtt ccgcgcatcg cgcgcgagtt gcgcgtcgag ggcggtcgcg cggtcacgcc
     2821 cgggaggtcg tggctcgccg tcaccgcctg cgccgcgctc gtgctgttcg tcgcgctcgt
     2881 actgcggctg gcgctgtggc cgaccgtttt ctacgacgac ctggtctacc atctcggcgt
     2941 gccgcgccag gcgctgttga ccggcacgtg gccggcgatg cagggcctgc actactcgtt
     3001 catgccggcc ggatgggacg cgacctacgt gctgccgctc gcgttcggcg ccggcagcgc
     3061 gccgcagctg atgaacgtga tcgccctcgt ctcgttcgcc tgggcggtct accggctggc
     3121 gcgcttcggt ggcgggcccg acggcgcggc ggtcgcgacc gcactgctcg tcatcgcgcc
     3181 ggtcaccggc tcgtcggggg cgttcgccgg caacgatctg ttcgtcgcgc tggcgctggc
     3241 ggtcgcggcc gagcggctgg tggcgacggc ggcgagccgc ccgcggctgg tcggcctgtt
//
LOCUS       DASPGN010000322         6989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_760422, whole genome shotgun sequence.
ACCESSION   DASPGN010000322 DASPGN010000000
VERSION     DASPGN010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760422"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VD788_09375"
     CDS             complement(<1..763)
                     /locus_tag="VD788_09375"
                     /inference="COORDINATES: protein motif:HMM:NF017071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXV76517.1"
                     /translation="MSTALHKTTHAFDEAKKAGRAPYKVADLKLAEFGRKEIRLAEQE
                     MPGLMSIRSKYRDDKPLAGAKVMGSLHMTVQTAVLIETLVDLGADVRWVSCNIFSTQD
                     HAAAAVVVGTEGTADNPRGVPVFAWKGETLEEYWSLTERALDFGEGRGPTQIVDDGGD
                     ATLLIHKGVEFEQAGRVPDPETAESEELAVVLRLLARLRERQPERWRRVAAECRGVSE
                     ETTTGVHRLYEMQRAGTLLFPAMNVNDSVTKSKFDN"
     gene            complement(799..1743)
                     /locus_tag="VD788_09380"
     CDS             complement(799..1743)
                     /locus_tag="VD788_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXV76518.1"
                     /translation="MDTTHRPQIFDQMSSLADRVRSRILLAVEDRELTVSELCSVLQL
                     PQSTVSRHLKTLGDTGWVLHRPDGTRRLYQFTPAELDATARDLWALTRAQVQATASAL
                     EDGRRLAGVLSRRRTRSREFFSEAAERWDRVRDEMFGSGFHALALLGLLDPRMRVADL
                     GCGTGAVSAALAPVVEQVFAVDDSASMLAAAAERLAGFDNVDLRQGQLEAVPLDDDSV
                     DAATLVLVLHHIPEPARVLSEVARVLIPGGRLLIVDMLPHERSELQQEMGHVWMGFDE
                     PRLGRFLAPARLELSRFIVIPPDADAMGPTLFAATAAA"
     gene            1886..3556
                     /locus_tag="VD788_09385"
     CDS             1886..3556
                     /locus_tag="VD788_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV76519.1"
                     /translation="MTRDLRAWVGKRRVLWIGFFAVLVPLAVLLAMQYGWLTRLEKTS
                     AIAEKAWLNNYLEAVSSKVEYFYRKQAERALNVPESLLAYQKPEKIGRFFEKKQVEGA
                     KYLFVYYDPPDSDGDPFMAFYDPVNGSVEQPADHRMLRAITVALSPWTMLLSKSESLS
                     SHALSVDEKDPENRIILNPLSDDSCTIIGIAGMIVDNGHFEQRVLPGAIEQALPKFFD
                     RRAADNLALSVLDGRGDLVLGLDGGARQMQGVARSIPFIYSDWKLVLGSRHTTPEQWA
                     KSNFVLNVSLSVLLAIVLLGGIAMALRTASRQIYLSQMKSDFVSNVSHELRTPLASIR
                     VFGEFLRLGRVADPAKTREYGEFIETESRRLTQLINNILDFSKIESGAKTYRFERVDL
                     AEVLSDTVRSLEVSLRHKGFRLGFDTPVDPVPVEIDPDAIAQAVANLVDNAVKYSNGA
                     REVDVRLERDEREVVISIRDFGIGISGDEQDKIFERFHRVSTGLVHDVKGSGLGLSIV
                     SHIVKAHSGEVRVESEPGRGSIFSIHLPAEHLPETRPTGLTAARADSG"
     gene            3595..4275
                     /locus_tag="VD788_09390"
     CDS             3595..4275
                     /locus_tag="VD788_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV76520.1"
                     /translation="MPRVLIVEDDEAMAVALRDGFTYEGYEVRMANDGASGLRLAAEG
                     DHDLIIMDVMLPRMNGLDICRRIRRDGSRVPLIMLTARGQEIDKVLGLKTGADDYVTK
                     PFSFLELMARVEALLRRSSGFAEPVDHCRFGDVEIDFRRAEARKGGRTVVLSPREFKL
                     IQFLVEHRGEVVTRERLLDAVWGYENVPFTRTVDMHIAKLRKKIEDRPHDPEHIVTVH
                     RVGYKFTG"
     gene            4406..5029
                     /locus_tag="VD788_09395"
     CDS             4406..5029
                     /locus_tag="VD788_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV76521.1"
                     /translation="MSRKPVFTTFETAKLCHVSPLSIINWVNAGKLPAFRTPGGHRRI
                     RREDLVRFMRENGIPIPDDLREGSGRFKVLVVDDEAGIRDVIAEHLTGRAKAYEVMTA
                     ADGFEAGRLMASFRPDVVLLDLRMPGLDGFQVCRTIKADPETAGTIVIAMTGYYSPDT
                     ESRISECGAVRCFAKPIDPATLSAYIDSIVEQKTSGSKTRRRRGVRG"
     gene            complement(5033..5968)
                     /locus_tag="VD788_09400"
     CDS             complement(5033..5968)
                     /locus_tag="VD788_09400"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HXV76522.1"
                     /translation="MRRPAAALAGAVVLLVATLACTEPDETVTVDIGTTRPALADSDL
                     ATQVVEVGIGVEGTIQAIVWNSVEAFEVAGLVEGPTDLTFDEPCSFIDTAVVGALFVG
                     KCGGGLIVESDSQPRPAVALETFSVNQITVHRVRPLSLDPGLDQDVDGVPNASDNCVL
                     VNNPQQTDTGMKGFGDACQAFDPFFGLPLVDNDADGVPDFVDNCPATKNPDQADDGTP
                     VGVPPDEIFVRDGMGNACAADHQTAVVQVDNDGWTFPVLTDFIQPFRANSWLTVDFND
                     QSALDCDWNAGTCTIVQKNVRFCFNVTGGFACDPS"
     gene            complement(5995..6681)
                     /locus_tag="VD788_09405"
     CDS             complement(5995..6681)
                     /locus_tag="VD788_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76523.1"
                     /translation="MRSLWIFVLGAVLVVAAGCTNSESLDSGAAHVPVRVEVENGDTR
                     FPTRAFLRIRSMRILPVDPLADAVHGATDPPAPLLLLSDDTAVSIDLNGDPPFFVSGA
                     PLPVGFYKITRITIDSFDFLNGLPANDGTCPGSITNFPFVPNAVSFTFPAADQHVFEV
                     QAGAENTVTAVIDGQAFLEALEESFSCRSGPLTVRCRTLSPPCTPTTWSSQVNTPALL
                     PFFNTFLSFR"
BASE COUNT         1175 a         2306 c         2368 g         1140 t
ORIGIN      
        1 ggttgtcgaa cttgctcttg gtcacgctgt cgttcacgtt catcgccggg aacagcagcg
       61 tgcccgcgcg ctgcatctcg tagagccgat gcacgccggt ggtcgtctcc tcgctgacgc
      121 cccggcactc ggccgccacg cgccgccagc gctcgggctg ccgctcgcgc aggcgcgcca
      181 aaagccgcag caccaccgcc agctcctcgc tctcggccgt ctccgggtcc ggcacgcgcc
      241 cggcctgctc gaactcgacg cccttgtgga tcagcagcgt cgcgtcgccg ccgtcgtcca
      301 cgatctgcgt gggcccgcgg ccctcgccga agtccagcgc ccgctcggtg aggctccagt
      361 actcctcgag cgtctcgccc ttccaggcga agaccggcac gccgcgcggg ttgtcggcgg
      421 tgccctcggt gccgacgacg accgcggcgg cggcgtgatc ctgggtcgag aagatgttgc
      481 acgagaccca acgcacgtcg gcgccgaggt cgacgagcgt ttcgatcagc accgccgtct
      541 ggaccgtcat gtgcaggctg cccatcacct tggcgccggc cagcggcttg tcgtcgcggt
      601 acttgctgcg gatcgacatc agaccgggca tctcctgctc ggccagtcgg atctccttgc
      661 ggccgaactc ggccagcttc aggtcggcga ccttgtacgg agcgcgacct gctttcttcg
      721 cctcgtcgaa ggcgtgtgtc gtcttgtgta gggcggtgct catcggttct ctccttgaaa
      781 acggtttggc gaatcgtatc aggcggccgc ggtggccgcg aacagcgtcg gacccatcgc
      841 atccgcatcg ggcgggatga cgatgaaacg ggacagctcg agccgcgccg gcgcgaggaa
      901 tcggcctagc cgcggctcgt cgaagcccat ccagacgtgg cccatctcct gctgcagctc
      961 gctgcgttcg tgcggcagca tgtcgacgat cagcaaccgg ccgccgggga tcaggaccct
     1021 cgcgacctcg gacagcaccc tcgccggctc cgggatgtgg tgcagcacga ggaccaacgt
     1081 cgcggcgtcg accgaatcgt cgtcgagcgg aaccgcctcg agctgtccct ggcgcaggtc
     1141 gacgttgtcg aagccggcga gacgctcggc ggcggccgcc agcatcgacg ccgagtcgtc
     1201 gacggcgaag acctgctcga ccaccggtgc gagcgccgcg ctgacggcgc cggtcccgca
     1261 accgaggtcg gcgacgcgca tccgcgggtc gagcagaccg agcagcgcca gcgcatggaa
     1321 ccccgagccg aacatctcgt cacgcacgcg gtcccagcgc tctgcggcct ccgagaagaa
     1381 ctcgcgggac cgagtgcgcc gccgggacag cacgccggcg agccggcggc cgtcctcgag
     1441 cgcggaggcg gtggcctgta cctgggcccg cgtcagggcc cacagatcgc gggcggtcgc
     1501 gtcgagctcg gccggcgtga actggtagag ccgtcgcgtg ccgtcggggc ggtgcagcac
     1561 ccagccagtg tcgcccaggg tcttgaggtg gcggctgacc gtgctctggg ggagctgcag
     1621 cacggagcac agctcgctga cggtcagctc gcggtcctcg accgcgagca ggatgcggct
     1681 ccggacacga tcggcgagcg acgacatctg gtcgaaaatc tgcggccggt gggtggtgtc
     1741 catgttctat ccgtcaatcc ggatagaatg atacaacagg tcggacgaag gtccagccct
     1801 ttcgcgtcgt aaaaacttgt tcggcttgta acgccttcgt aaagccggcc ggtgcccccc
     1861 cttcgctcgg cgtatcattg cccgcatgac gcgcgatctg cgggcctggg tcgggaagcg
     1921 ccgcgtgctg tggatcggct ttttcgcggt tctcgtgccg ctggccgtcc tgctcgcgat
     1981 gcagtacggc tggttgaccc ggctcgagaa gacgtcggcc atcgccgaga aagcatggct
     2041 caacaactac ttggaggcgg tgtcgtccaa ggtcgagtac ttctatcgca agcaggccga
     2101 gcgtgcgctc aacgtcccgg agagcctgtt ggcctaccag aagcccgaga agatcggccg
     2161 tttcttcgaa aagaagcagg tcgagggcgc caagtacctg ttcgtctact acgacccccc
     2221 cgacagcgac ggcgacccgt tcatggcctt ctacgacccg gtcaacggat cggtcgagca
     2281 accggccgac caccgcatgc tccgcgcgat caccgtcgcg ctttcgccct ggacgatgct
     2341 gctcagcaag tccgagtcgc tctcgtcgca cgcgctctcg gtcgacgaga aggaccccga
     2401 gaatcgcatc atcctcaatc cgctgtccga cgacagctgc acgatcatcg gcatcgccgg
     2461 gatgatcgtc gacaacggcc acttcgagca gcgcgtcctg cccggcgcca tcgagcaggc
     2521 gctgccgaag ttcttcgacc ggcgggcggc ggacaacctg gcgctgtcgg tgctcgacgg
     2581 ccgcggcgac ctggtgctgg gactcgacgg cggcgcccgg cagatgcagg gcgtcgcgcg
     2641 atcgatcccg ttcatctatt ccgactggaa gctggtgctc ggcagccgtc acacgacacc
     2701 cgagcagtgg gccaagtcga acttcgtgct caacgtctcg ctgtcggtgt tgctcgcgat
     2761 cgtgctgctc ggcgggatcg cgatggcgtt gcgcacggcc tcgcggcaga tctacctgag
     2821 tcagatgaaa tccgatttcg tttcgaacgt gtcgcacgag ctgcgcacgc cgctggcttc
     2881 gatccgcgtg ttcggcgaat tcctcaggct cgggcgcgtc gcggatccgg ccaagacgcg
     2941 cgagtacggc gagttcatcg aaaccgaaag tcgccggctg acgcagctga tcaacaacat
     3001 cctcgacttc tcgaagatcg agtccggcgc gaaaacgtac cggttcgagc gggtcgacct
     3061 ggccgaggtg ttgtcggaca ccgtgcgttc gctcgaggtc agcctgcgcc acaaagggtt
     3121 tcgcttgggg ttcgacacgc ccgtcgaccc ggtgccggtc gagatcgatc cggacgcgat
     3181 cgcgcaggcc gtcgccaacc tggtcgacaa cgccgtcaag tactcgaacg gcgcacgtga
     3241 ggtcgacgtg cggctcgagc gagacgaacg ggaagtggtg atctcgatcc gggacttcgg
     3301 catcggcatc tccggcgacg agcaggacaa gattttcgag cggttccatc gcgtgagcac
     3361 cggtctggtt cacgacgtca agggcagcgg tctcgggttg tcgatcgtga gccacatcgt
     3421 caaggcgcac tccggcgagg ttcgcgtcga gagcgagccg gggcgcggca gcatcttctc
     3481 gatccacctg ccggccgaac acctgcccga gacgcgaccg accggtctga ccgcggccag
     3541 ggcggattcc ggatgaccgg tcgacagccg cgggcgagca ccgaggacaa accgatgccg
     3601 agagtgttga tcgtcgagga cgacgaggcg atggccgtcg cgctccgcga cggtttcacc
     3661 tacgaaggat acgaggtgcg gatggcgaac gacggcgcga gcggtctgag gctcgccgcc
     3721 gaaggtgacc acgacctgat catcatggac gtgatgctgc ccaggatgaa cggtctcgac
     3781 atctgcaggc ggatccgccg ggacggcagc cgcgtcccgc tgatcatgct gaccgcccgc
     3841 gggcaggaga tcgacaaggt gctgggtctc aagaccggcg ccgacgacta cgtgacgaag
     3901 ccgttcagct ttctcgagct gatggcgagg gtcgaggcgc tgctgcgccg cagctcgggc
     3961 tttgccgagc cggtcgacca ctgtcgtttc ggtgacgtcg agatcgactt tcgtcgcgcg
     4021 gaggcgcgca agggcggccg gaccgtggtg ctttcgccgc gcgagttcaa gctgatccag
     4081 ttcctggtcg agcaccgcgg cgaggtcgtc acccgcgaac ggctgctcga cgccgtctgg
     4141 ggctacgaaa acgtcccgtt cacgcggacg gtcgacatgc acatcgccaa gctgcgcaag
     4201 aagatcgagg accggccgca cgatcccgag cacatcgtca ccgtgcaccg ggtcgggtac
     4261 aagttcaccg gctgagccgc ttcggacgtc gggactggcg cgacatccga gttacagttt
     4321 tggcgcctcg gacttgacat cgtcccgagg atcgcagata ttattcgagt agctttgaat
     4381 tgttaaaaaa gctcggtacg ggacgatgag tcgaaagccg gtattcacca cgttcgagac
     4441 ggcgaagctg tgtcacgtct ctccgctctc gatcatcaac tgggtcaacg cggggaagct
     4501 gcccgctttc cgcacccccg gcgggcaccg ccgcatccgc cgtgaggatc tcgtccgttt
     4561 catgcgcgag aacggcatac cgattccgga cgatctgcgc gaggggtcgg ggcggttcaa
     4621 ggtgctggtc gtcgacgacg aggcggggat ccgcgacgtg atcgccgagc acctgaccgg
     4681 gcgtgccaag gcctacgagg tgatgacggc ggccgacggc ttcgaggcgg gccgcctgat
     4741 ggcgagcttc cggcccgacg tcgtgctgct cgacctgcgg atgccgggtc tcgacgggtt
     4801 ccaggtctgc cgcacgatca aggccgatcc cgagacggcg gggacgatcg tcatcgcgat
     4861 gaccggctac tactctccgg acaccgagtc gagaatcagc gaatgcggcg ccgtgcgctg
     4921 cttcgccaag ccgatcgacc ctgcgacgct gtcggcctac atcgactcga tcgtcgagca
     4981 gaagacctcg ggctcgaaga cgcgccgacg ccggggtgta cgcggctgaa cgtcacgacg
     5041 ggtcgcaggc gaagccgccg gtgacgttga agcagaaccg cacgttcttc tgcacgatcg
     5101 tgcaagtgcc ggcgttccag tcgcaatcga gcgccgactg atcgttgaag tcgaccgtca
     5161 gccaggaatt ggcgcggaac ggttggatga aatcggtcag cacggggaag gtccatccat
     5221 cgttgtcgac ctgcacgacc gccgtctggt ggtcggcggc acaggcgttg cccatgccgt
     5281 cgcggacgaa gatctcgtcg ggcggcacgc cgaccggggt tccgtcgtcc gcctgatccg
     5341 ggtttttcgt ggccgggcaa ttgtcgacga aatccggcac tccgtccgcg tcgttgtcga
     5401 cgagcggcag cccgaagaac gggtcgaacg cctgacaggc gtcgccgaat cccttcatcc
     5461 cggtgtccgt ctgctgcgga ttgttgacca gcacgcagtt gtcgcttgcg ttcggcacgc
     5521 cgtcgacgtc ctggtccaag ccggggtcca agctcagggg tcggacgcgg tggacggtga
     5581 tctggttcac cgagaacgtc tcgagcgcca ccgccggccg cggctgactg tccgactcga
     5641 cgatcagccc gccgccgcac ttgccgacga acagcgcccc gaccaccgcc gtgtcgatga
     5701 acgagcaggg ctcgtcgaag gtcaggtccg tcggaccctc gacgagaccg gccacttcga
     5761 acgcctcgac ggaattccac acgatggcct ggatcgtgcc ttcgacgccg atccccacct
     5821 cgaccacctg cgtggccagg tcggagtcgg cgagcgcggg gcgggtcgtc ccgatgtcga
     5881 ccgtgacggt ctcgtcgggt tcggtgcagg cgagcgtcgc cacgagcagc actaccgcac
     5941 cggcgagggc cgcggcggga cgtctcatcg cgcgggtcgt tgcacctgaa gcgttcagcg
     6001 gaacgacagg aaggtgttga agaagggcag caaggccggg gtgttcacct gagaggacca
     6061 cgtggtcggc gtgcacggcg gagacagggt ccggcaccga acggtgagcg ggccgctccg
     6121 gcaactgaac gactcttcga gcgcctcgag gaaggcctgc ccgtcgatca ctgcggtcac
     6181 ggtgttttcg gcgccggcct gtacctcgaa gacgtgttga tcggccgcgg ggaacgtgaa
     6241 cgacacggcg ttcggcacga acggaaaatt cgtgatcgac ccggggcagg tgccgtcgtt
     6301 ggccgggagg ccgttgagga agtcgaagct gtcgatcgtg atccgtgtga tcttgtagaa
     6361 cccgaccggg agaggggcgc cggagacgaa gaacggcgga tccccgttca ggtcgatcga
     6421 gaccgccgtg tcgtcgctga gcagcagcag tggcgccggc ggatcggttg cgccgtgcac
     6481 cgcgtcggcg agcgggtcca ccgggaggat gcgcatgctg cggatccgca ggaacgcccg
     6541 ggtcggaaac cgcgtgtcgc cgttctcgac ctcgacgcgc accgggacat gcgccgcccc
     6601 ggaatccaac gattccgagt tggtgcagcc cgccgccacc acgagcacag caccgagcac
     6661 gaagatccac agcgatcgca tccgctccga cctcatctcg caagccccct cgacccgcca
     6721 cgctccggcg cgagccggga aaaccccacc atacgaccgg gccggcgcgg acgcccaact
     6781 atagccgaat cattgctgcg agaccaccgc gcgggcggag cggatgcgac gccggacagg
     6841 cccctaggcc taggagcctg tccgagtaac cggggctgat tgagttcggc tggcggtagc
     6901 gagcatgatc atccaatcgt gcagtcgaga tcggcggcgc cgcgggcgcc gaacgactcc
     6961 gtgctgaggt tggcccctgt cgctggggt
//
LOCUS       DASPGN010000323         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_526330, whole genome shotgun sequence.
ACCESSION   DASPGN010000323 DASPGN010000000
VERSION     DASPGN010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526330"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1917)
                     /gene="ppdK"
                     /locus_tag="VD788_09410"
     CDS             complement(<1..1917)
                     /gene="ppdK"
                     /locus_tag="VD788_09410"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HXV76524.1"
                     /translation="MSKLVYTFANGKAEGDSGKKDLLGGKGANLAEMTNLGIPVPPGF
                     TISTHACNQYVEGGHKMPPRLRVQVDRALAGLERVQRKRFGDTGDPLLVSVRSGAKFS
                     MPGMMDTILNLGLNDAAVEGLARVSGDRRFAFDSYRRFIQMYADVVLGVAKHRFETLL
                     GDAKRKRRVELDTELSASDLETLTAGYKRLVKQATGKAFPQDARQQLWGAIAAVFRSW
                     ENPRARRYRREYGIADDLGTAVNVQCMVFGNLGDDCATGVAFTRDPATGANLLYGEYL
                     VNAQGEDVVAGIRTPQPISQSQRVDESQVSLERRLPEAYAQLQKIRAKLEKHYKDMQD
                     VEFTIERGRLYMLQTRTGKRTGLAALNIAYELQRARVISKREAVKRVEPDMLVQLLAP
                     VFDPKEVDRARQSGRVLARGLPAGPGAATGAVVFTAERAEAWVAEGKTVLLVRTETSP
                     EDIGGMVAAAGILTSRGGMTSHAAVVARGMGKPCIVGAESLIVDEARRRVSAGPLVLK
                     EGDRLSIDGATGEVIRGELEPHPSEIQQVLVERSLKPAKSELYKRFSSLMQWADQVRR
                     LGVRTNADTPKDSAIARAFGAEGIGLCRTEHMFFAEDRIAAVREMILSDDDEHRDRAL
                     KRLLPMQRRDFIGIF"
     gene            complement(1914..>2277)
                     /locus_tag="VD788_09415"
     CDS             complement(1914..>2277)
                     /locus_tag="VD788_09415"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HXV76525.1"
                     /translation="RVDNILTKNGSAIERIVRGGSAGSPASEPVEAERALARTVERLV
                     PRIDLASRRKCYDDVVDSLAEFIDPVERFFTDCLVVDPEDPETTRRRHALLIDLKQLF
                     TKYFDIRELAGEADGEAQ"
BASE COUNT          356 a          782 c          735 g          404 t
ORIGIN      
        1 gaaaatgccg atgaagtcgc gtcgctgcat cggcagcagt cgtttgagcg cgcgatcgcg
       61 gtgctcgtcg tcgtccgaca ggatcatctc ccgaaccgcg gcgatgcggt cctcggcgaa
      121 gaacatatgc tcggtccggc acaagccgat accctcggcg ccgaacgccc gggcgatcgc
      181 cgagtccttc ggcgtgtccg cgttggttcg tacgccgagg cgtctcacct gatcggccca
      241 ctgcatcaat gacgagaagc gcttgtacag ctcggacttc gccggcttga gcgagcgctc
      301 gaccagcacc tgctggatct cggagggatg cggctcgagc tccccgcgaa tcacctcgcc
      361 cgtcgcaccg tcgatcgaca gacggtcgcc ctccttgagc accagcgggc ccgcactgac
      421 gcgccgccgc gcttcgtcga cgatcagcga ctcggcaccg acgatgcagg gcttccccat
      481 tccgcgtgcg acgaccgcag cgtgcgacgt cattccgccg cggctggtga ggataccggc
      541 ggcggcgacc atcccgccga tgtcctccgg gctggtttcg gtccgcacga gcaagacggt
      601 cttgccctcc gcgacccagg cttcggcgcg ctcggcggtg aagaccaccg cccctgtcgc
      661 cgcaccggga ccggcaggca gtccacgggc gagcacccga cccgactgac gcgcgcggtc
      721 gacctctttc gggtcgaaca ccggggccag caactgcacc agcatgtcgg gctcgacccg
      781 cttgaccgcc tcacgcttcg agatcacgcg cgcacgctgc agctcgtagg cgatgttgag
      841 cgcggccaga ccggtacgct ttccggttcg cgtctgcaac atgtagagcc ggccgcgctc
      901 gatcgtgaac tcgacgtcct gcatgtcctt gtagtgcttt tccagcttgg cgcggatctt
      961 ctgcagttgg gcgtaggcct ccggcagccg ccgctcgaga ctgacctgac tctcgtcgac
     1021 gcgctggctc tgcgagatcg gctgtggggt gcgaatcccg gccaccacgt cctcgccttg
     1081 cgcgttgacc aggtactcgc cgtacagcag attggcgccg gtcgctgggt cacgcgtgaa
     1141 cgccacgccg gtggcgcaat cgtcccccag gttgccgaag accatgcact gcacgttgac
     1201 cgcggtgccg aggtcgtcgg cgatgccgta ctcgcgacgg tagcgccggg cgcgcgggtt
     1261 ctcccagctg cggaagaccg cggcgatcgc gccccacagc tgttgtcgcg catcctgcgg
     1321 gaaggcctta cccgtcgctt gcttgacgag gcgcttgtag cccgcggtaa gcgtctcgag
     1381 gtcggaggcg ctcagttcgg tgtcgagctc gacgcggcgc tttcgtttcg cgtcgccgag
     1441 cagggtctcg aagcgatgct tggcgacgcc tagcacgacg tcggcataca tctggatgaa
     1501 ccggcggtag gaatcgaagg cgaaccgccg gtcgccgctg actcgagcca ggccctcgac
     1561 ggcggcgtcg ttcaacccga ggttgaggat cgtgtccatc atgccgggca tcgagaactt
     1621 ggcgccggac cgcaccgaga ccaacagcgg gtccccggtg tcgccgaatc gcttgcgctg
     1681 cacgcgctcg agaccggcca gcgcgcgatc gacctgcacg cgcagtcggg gcggcatctt
     1741 gtggccgcct tcgacgtact ggttgcacgc gtgcgtcgag atggtgaaac ccggagggac
     1801 cggaatgccg aggttggtca tctcggccag gttggctccc ttgccaccca acaggtcctt
     1861 cttcccggaa tcgccttcgg ccttgccgtt ggcgaaggtg tagacgagct tgctcactgc
     1921 gcttccccat ctgcctcacc ggcgagttcg cgaatgtcga agtacttggt gaacaattgc
     1981 ttgagatcga tcaacaacgc gtggcggcgg cgagtcgtct cgggatcctc cgggtcgacc
     2041 accaaacaat cggtaaagaa ccgctcgacc gggtcgatga actcggcgag actgtcgacc
     2101 acgtcatcgt agcactttcg tcgagaggcc aagtcgatgc gaggcacgag ccgctcgacg
     2161 gttctcgcca gtgcccgctc ggcctcgacc ggttcgctcg ccggcgaacc ggcgctcccg
     2221 ccgcgaacga ttcgctcgat cgcgctcccg ttcttggtca ggatgttgtc cacgcgc
//
LOCUS       DASPGN010000324        17025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_58008, whole genome shotgun sequence.
ACCESSION   DASPGN010000324 DASPGN010000000
VERSION     DASPGN010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 17025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17025
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58008"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(319..1191)
                     /locus_tag="VD788_09420"
     CDS             complement(319..1191)
                     /locus_tag="VD788_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76526.1"
                     /translation="MNRAVLVLLLLLPGAVAAQSELPCDYHDPKWLPNGERIAFYSNR
                     AGDFDVYTIRADGSDLLNVTRHPARDSEFAWAPAGDRLVFKSNRDGQGAYYLASSDGT
                     GVQPLSADLGEVGAPRWTPDGRALVYEGRVGDNRDIFVVELEGLARRRVTSDTARDFR
                     PAVSPDGRWLVFQSNRSGAYHLYRMSLTGGESEALTVGPFNDASPTWSPDGSRLAFSS
                     DRSGNEDIYVMTIGDTTVVQVTTDEALDYAPEWSPDGKSLVFISSRSGRSGVYVVRLD
                     GTPPRLLIEGCVAK"
     gene            complement(1280..2041)
                     /locus_tag="VD788_09425"
     CDS             complement(1280..2041)
                     /locus_tag="VD788_09425"
                     /inference="COORDINATES: protein motif:HMM:NF025027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HXV76527.1"
                     /translation="MARRKTTPLPATPTQKLEALVARAVDLAKTARRNHMSLAGSNYY
                     ANKLVELRADATNLYRELAPLTAGDSSALGELLERVFATSTEAKARAEAARELTFSLR
                     TTWRSANPAPREDDGLFPLSILSQSGRGYLVTVGRQMNGCFSQGWYDASAVMMRRLLE
                     IAIIEAFEAKGVAAKIKDANGDYLHLSDLVGRALGEASWTLSRNCKKALPALRDAGHT
                     SAHGRYYHARREDLEGLRQGCRVVIEEFLHHAGLQ"
     gene            complement(2045..2476)
                     /locus_tag="VD788_09430"
     CDS             complement(2045..2476)
                     /locus_tag="VD788_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76528.1"
                     /translation="MKQSKPYAELVTQAEEATKAIKDPELRRVAFERVLDDLLAGGGT
                     ATESQARSKKPPQRARTAAKAKSSRGPKGYIRELFDDGFFKKPKTISQVKAELGNRGH
                     HIALTSLSGPLQSLCKDRVLRRHKAKAGEKGTKVTYNYSEW"
     gene            complement(2646..3038)
                     /locus_tag="VD788_09435"
     CDS             complement(2646..3038)
                     /locus_tag="VD788_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76529.1"
                     /translation="MSLVSQVVLALQVGSVGVGLAPQIRVPLRRVSWFVGLIVGPPLL
                     WTLCATSVDLVLSFESMHHFSLDPLYAWLLKLPTIIGTTLFLQCLLAWQYADSPMRSL
                     PIVGVAFGLLAILGDVVFVLCMVTALGP"
     gene            3876..5795
                     /locus_tag="VD788_09440"
     CDS             3876..5795
                     /locus_tag="VD788_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV76530.1"
                     /translation="MSHPRGSSKQRRKTSRAAGELRGKLRPHDPFELIRWLALSQPDP
                     RKALAELVQNSLDADAHRIRITRKRERGTPCLRILDDGAGVIPELNRPDALKYIATHI
                     GHSRKRSLSPQERLQLMTQGQYGIGLLGFWSLGELLEIRSSVPDQRPHRLVLHRDRPD
                     YRIEPLRGNLALDERFTEVVVAGLHREALGALIGRRAADYLKSELRGQLLARAVDLVV
                     EDRMSRGRAQRILTVKPQRFLGERLEGLGPIDVPGHAPIRLEVYVAGEGGDPENGRLS
                     LYSAGTLVAEDFHAMAPLGLDRPPWTDPRLTGIVDFPSFRVAPGSRRGVVLDEAAGAF
                     AAALSAVDTVLLGVLDAIERRRAEELDRSIVRDLQRAFRDFHRHRPRYSMLPVAERPG
                     PAAGTSPGKADDGGGGAVAAESPSPLAPSQSSGRSPREPAVDLLPPGPLDGVRVSPAN
                     MLVECRGSREARAQAVDAVGRPIEDTVEFRWLVVPAMLGALEVGTGGSTAESRARLAA
                     GEHEGSGTLEVTAVSGERRAQATIGIEVVDEIPGGRGDEGIPEPELVDLPGAGWRSRM
                     HDGLWQVNSGHRDFRAAAASPVLKLRYLAMLFSKEVVLRSHQDPRLDQPLEQLVEVAA
                     YADRKLLRKGRRKKG"
     gene            complement(5800..7554)
                     /locus_tag="VD788_09445"
     CDS             complement(5800..7554)
                     /locus_tag="VD788_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HXV76531.1"
                     /translation="MEGHRFDSEAEAGENGSGRPVLVVSSDGATLTWGTRWLGRVGFQ
                     VETARTVDDALRRVEAGAFGLVLFDDELDRTSAEPFCGRLRGLPAARGVPVLSLCTGT
                     SQALAALESGSTDVVTRPYDWRVLIRRADLLYRSHRIDRIQRSRLEPPGLLTGLPNHA
                     AFRQTLAAAVAKSDRVAVMSIGLDRFRSVNQAHGRDAGDRILRLVAGRLTGSVRSSDR
                     LEDPPSALAHFSGDDFKMMFAGLRRPEHAMTIAKRLHRALSDPYVVDRKELFVSASIG
                     VAISPADGVDAETLLHRAETALETAKRDGGGLVRGCSGPVDDGNERRLLVGRHLRGAI
                     DRGELELHYQPLVNLGSGRVVGAEALLRWHNEKLGSVSPAEFVPLAEESGLIDSIGRW
                     VLATACRQLRAWLDEGLPPIRVSVNVSARQLVSGDLADVVRGVLRDSGIDGRLLELEL
                     SERGALRSDPAVLEQMRALKAMGVRLSVDDFGTGNSAIAYLRRFPLDALKIDRSYVRG
                     VESSENDAAITSAMIAMAHKLRLVVVAEGVEQSGQLAFLRDCRCDQYQGFLFSAAVPA
                     ASFRELLGDRAMPAELLN"
     gene            7820..10687
                     /locus_tag="VD788_09450"
     CDS             7820..10687
                     /locus_tag="VD788_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXV76532.1"
                     /translation="MSRLLRTSRARAATLYLPPPADLHGRALLVHEGEAAPLPELADP
                     EAAEQFCRGLSDANLAHRDPVESGGRGGWLIALPALDDLLSADSDGEARRSPGRRRHE
                     SLHPESGPTQTAWLGLQFDADTSASDRLPAALSVLRRDRASGWHWLVAVGGVLAWHGQ
                     HVSSLLREPVSRLPGRTSLHGVLRAMLAAAARDEARVALLLVNPDAFALVNERFGREA
                     GDGVIREIAGRLERALRQTDFVCRYGGAVFGVALPDAGEREIETVAAKLRDAICRDGF
                     LDGALRLSTSFGGASHAPDRDEDLTEAMLRLVRRADRALNSASKSGGGRTVIWRRDRE
                     LEEIGQFDRLSGIFTANPSKDYRNMMLLWDTVHAVASSDDVARLIDRVLERLVAAFNA
                     ARVMLVEHTDAGGYGPARAASRGGERGAATEGERRFAPDHELRALLDQASLERRALER
                     PLSAGRLGCAVPLASDDRRLGLLYLEGDAHEFQLDSSDRILLGGLAGQLAIALDRARL
                     AEAERLRLDDERRTLRRELSELRRALGDAKLVHRSEAMDAVLDVARRVAPTDATVLIC
                     GESGTGKELVARTIHALSARRDGPLVVVDCSAIAVSLIDSELFGHEKGAYTGAERGST
                     GRLAEADGGTILLDEIGELPLEVQGKLLRFVQDRQFVAVGGTRPRRVDVRLIAATNRD
                     LAAEVKIGRFREDLYYRLMVVQLTVPPLRERPEDVVLLARHFVDSFALQYGRQPRRLT
                     AEAEVHLTAQGWPGNVRELQNRVMQAVILSRQERIGVDELTTGRPTPARTAADRAAGT
                     AVPAPAEAADGDAWDTLRAVLRQRIALAANAAAPPPIGRWVADDLILEAHRRSGRVAA
                     HAARALGIAKTTFRRRLRKAAIEAERGTSPRPNDWAEVLASLARIVAAANDDGEDRTR
                     RVRLLLLEEVERRVTRDTGSAALLMGVSDPTYGRWVAELAG"
     gene            10684..11166
                     /locus_tag="VD788_09455"
     CDS             10684..11166
                     /locus_tag="VD788_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76533.1"
                     /translation="MTQKGKTMPKQVGDYILDCLRALNKDEGEVELGNRIHDVRQYWH
                     DLWGGAVPSGARIVAAFLSNEPGSREPKDLWFFSDSHCLLAHNVMSAHLDLLVYPFRN
                     GVHRLRVVRNYDDQPSPGSRLVVGLWFDEQTKVALEAGDGNCLPLETLLEEVLIPHLQ
                     "
     gene            11284..11943
                     /gene="thiE"
                     /locus_tag="VD788_09460"
     CDS             11284..11943
                     /gene="thiE"
                     /locus_tag="VD788_09460"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXV76534.1"
                     /translation="MDRDSGIGRLHVITDETVQTRWSHVELARAAALGGADRVQYREK
                     RPRTTRELVETARRIRRALDGTAARLVLNDRIDVAVAAGAGAVHLGRDDLDPAIARRL
                     VGDSMRIGATANDLEQALRAARLPVDYLGVGPVFGTRSKTAPAPALGLVRLAEIVRAV
                     PLPVIAIGNITVDHVAAVLATGVHGIAVLSAIACAHDPREATSGFARAIDHARSGPVA
                     G"
     gene            11951..13105
                     /gene="thiO"
                     /locus_tag="VD788_09465"
     CDS             11951..13105
                     /gene="thiO"
                     /locus_tag="VD788_09465"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HXV76535.1"
                     /translation="MTIRRTDVTVVGGGVIGLSVAAELLRRGVAVDVIDRGPIPGYAT
                     AAAAGMLAPVSEAELEDEVVLPLALDSLARFPAFVDWLERTTGLPCDYRTDGTLWVAV
                     TRDDRAELEHIHHTLTERIGGMPARWLSAGEVHAREPHLSGRVLCGLWVERDLQVDPR
                     ALTAALERAIVAAGGTIERGTRVVGIIADGGVVRAVELATPDGVARRAAGGVVLAAGA
                     WSGHELRLPLGDLGIRPVKGQLIRLAGAPLLRHVVRHPEAYLVPRGTGELLVGATMEE
                     VGFDPAVTAGAVHDILRKAWQLLPGLYDLELREVSVGFRPMSRDNRPVIGPTAIAGLY
                     LAVGHGRQGVLLAPATAQLLADAITTGRVPEVLLPFAIDRFGGATATVGG"
     gene            13111..13581
                     /locus_tag="VD788_09470"
     CDS             13111..13581
                     /locus_tag="VD788_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76536.1"
                     /translation="MDDFDFLVGRNWHVRNRVRVERLANCDEWTEFDARLLDVRKILG
                     GLGNIDRFSARRDGKPFEAVSLRLFNPSSGLWTIYWADNVSATLTEQVTGRFVDGVGE
                     FLGRETYRGQTVELRFRWTDVTERSPRWEQAYLDESTGEWETNWIMRFTELETR"
     gene            complement(13585..14352)
                     /locus_tag="VD788_09475"
     CDS             complement(13585..14352)
                     /locus_tag="VD788_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV76537.1"
                     /translation="MRPIHTRAVVRCSVLACLLIAGARDAGAQIPDEYTNLTVVPKEI
                     TKSELVGLMREVASSLGVRCAYCHVGPDDLQGMDFASDEKAVKRTARTMMKMVDEING
                     RHLKAIETGRAETTRVRCVTCHRGLTVPKPIQEILDAEIEANGVGAALAKYRELREQH
                     FGSAAYDFSQGPLNWIVEKLAREQRLDDALAIAAANVELNPTEAYARLLLGRLHVMRD
                     EREQAIAAFREALELDPENAWAKTQLEELTAAGDEQE"
     gene            14528..16174
                     /locus_tag="VD788_09480"
     CDS             14528..16174
                     /locus_tag="VD788_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV76538.1"
                     /translation="MRGTVRALRRRWRSAVTVALLSGTLVIVAVLTLQAQFAWTYHRT
                     TAENVLRDYSRLVAEEFIRRTSQEIGYRGFFSVVTALQQSLGGVAAPVLIELAEIPGG
                     LDDARAPRELVRTLFLVDPETGSVETKGVPLDAETAAVLRQRIGSSLPASGERAGPFS
                     AIQLGDEAGQRIVIVVPIAAPAGSSARLAGFEVDTAGLSTWLRRMVDSRPLLPASLGD
                     GELTNDLLFLRLSDATGRVIFTSGTWPGPLLATETPMGDAYGGVFDGLTLTVCIDPAA
                     APRLVIGGLPRSRLPVLSGLLLLTAGLLGAAVWQMRRERALARMRSEFVGRVSHELRT
                     PLTQIRMFAETLSLGRTRTPAEHRRSVEIIDQEARRLGHLVENILQFSREERGATCLA
                     PRPLELAPLVRDVIEKFQPVASARRVRLATDLVEDAMVLADDEAIRQVFLNLLDNAVK
                     YGPAGQEVRVGLERTGRMVRATVEDEGPGVPERDRERIWARWQRLDRDERRAISGTGI
                     GLTVVRELVSLHGGRTWVEPGRRRGARFVVELPVTAGGTA"
     gene            16171..16863
                     /locus_tag="VD788_09485"
     CDS             16171..16863
                     /locus_tag="VD788_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV76539.1"
                     /translation="MKRILIIEDNADLAFGLRNNFELEGYEVRVAGDGPAGLAALADA
                     EADLVVLDLMLPEMNGFDVLEQIRRRGSVVPVLILTARGEESDKVTGLRLGADDYLTK
                     PFGLMELLARVEALLRRSTATGEGRSRTKAILKFGAIEVDPATRTVTRDAAVVHLAPK
                     EFDLLVALIRHDGAVVSREELLDEVWGYASDVASRTVDTHIAELRRKLEHDPSMPRHL
                     LTVRKAGYRLAR"
BASE COUNT         2600 a         5836 c         5930 g         2659 t
ORIGIN      
        1 ccgtcacctc tcgaagctgc gcgatgcgct gagacgggag ctcaacatcc agaagaagac
       61 agcaggtaag gaagggaaga ggaagatcgc gtaaccaagg agccgtggaa actttcaact
      121 aactttgggc ttgactccgg catgacgact tcggaggccg caagaagagg gccacagaga
      181 ccggtcagta gcaccagagc gacaaatcga aagtgaccca cctgtgtgtc ccgtgtccgt
      241 ctaacgcccg ccgataacct gcgggcgccg aaggcgaccg tcaggttcat cggcttgtta
      301 gaccgcaacg tgtggcattc actttgccac gcatccttcg atcagcagtc gtggcggtgt
      361 gccgtcgagt ctgacgacat aaacgccgct gcgtccggaa cgcgacgaaa tgaacacgag
      421 gcttttgcca tccggcgacc actccggcgc atagtccaag gcctcatcgg tcgtcacctg
      481 cacgactgtg gtatcgccga tggtcatcac gtaaatgtct tcattcccac tgcgatccga
      541 cgagaaggcg agacgagaac catccggcga ccatgtgggg gaggcatcgt tgaatggacc
      601 tacggtgagc gcttccgact ctcctccagt caacgacatc cggtacaagt ggtaggcacc
      661 ggagcggttg gactgaaaca ccagccaccg cccatcgggc gagaccgcgg gtcgaaagtc
      721 tctcgcggta tccgaggtca cgcgacgacg cgccaagccc tccagttcaa cgacaaagat
      781 atcccgattg tcgcccacgc ggccttcgta gaccagcgct cgaccgtcgg gggtccatcg
      841 cggagcgcca acctctccaa gatcggccga cagtggctgc acacccgttc cgtcgctact
      901 cgcaagatag taggcgccct gaccatcgcg attcgacttg aagaccaggc ggtcaccggc
      961 cggtgcccat gcgaactctg agtcgcgggc gggatgtcgg gtaacattga gaaggtcgct
     1021 accatctgct cgtatggtat agacgtcaaa gtcacccgcg cggttcgagt agaaggcgat
     1081 gcgctcgcca ttgggcaacc atttaggatc gtgataatca cagggcagct cactctgcgc
     1141 agctactgcg ccaggaagca ataagagtag taccaacacg gcccgattca ctgacttcct
     1201 ccctttgcgg tctaacgtcc agcatcagcg gcggcgcgca gcgccgtccg ctgcatgctg
     1261 ttgttatgcc gggctctcgt cattgcaacc ctgcgtggtg aagaaactcc tcgatgacta
     1321 cccggcaccc ctgtcgtaat ccctcaaggt cttcacgccg ggcatgatag taccggccat
     1381 gggcggaagt atgacccgcg tcgcgaaggg ccggaagagc tttcttgcag tttcgcgaca
     1441 acgtccaact ggcctcgccg agtgcgcggc ccacgaggtc cgacagatga aggtagtcac
     1501 cgttcgcatc cttgatcttg gccgcgactc ccttcgcttc gaatgcctcg atgatggcga
     1561 tctcgagcag gcggcgcatc atgaccgcgc tggcatcgta ccaaccttgc gagaagcagc
     1621 cgttcatctg gcgccctacc gtgacgagat acccgcggcc ggactgactc aagatgctca
     1681 gcggaaacag gccgtcatct tcgcgcggag ccgggtttgc tgatcgccag gttgttcgca
     1741 aagagaacgt tagttccctt gccgcctcag ctcgtgcctt tgcctccgtg gaggtggcga
     1801 acacacgctc gagcaactcg cccagggcgg atgagtctcc cgccgtgagt ggggcgagct
     1861 cccggtatag attggtcgcg tccgcgcgaa gctcgacaag cttgttggca tagtagtttg
     1921 agcccgcgag gctcatgtgg ttccggcgag cagtcttcgc gaggtcgacc gcacgcgcaa
     1981 caagggcttc aagcttctgg gttggggtcg cgggtagcgg cgtcgtcttc ctacgggcca
     2041 cggcctacca ctccgagtag ttgtacgtga ccttcgttcc cttctcgccc gctttggcct
     2101 tgtggcgccg aagtactcgg tctttgcaca acgactggag aggaccactg aggctcgtta
     2161 gagcgatgtg gtgaccgcgg ttgccaagct ccgccttgac ctggctgatg gtcttcggct
     2221 tcttgaagaa gccgtcatcg aacagctctc ggatgtaccc tttcgggccg cggctactct
     2281 tcgccttggc cgccgttcgt gcgcgctgcg ggggcttctt cgaccgcgcc tgactctcgg
     2341 tcgcggtgcc accgccagca aggaggtcgt cgaggacacg ttcaaatgct acccgccgaa
     2401 gttccgggtc cttgatcgcc ttcgtggctt cctccgcttg cgtgacgagt tcggcgtacg
     2461 gcttcgactg tttcattctc gcgtgctcct ctatgtggcc tgagcaaccg gtctcacttg
     2521 cgttcctcgg cataacgtgt gcatcacctg cggccggccc aacgacgccg cgctggccaa
     2581 aggccagcgc cacgattgta cgccggccgt caggtgcatg cgcctgttag gcggccggcc
     2641 gcctttcacg gccctaacgc ggtcaccata cacagcacga acacgacgtc gcccaggatg
     2701 gccaataggc cgaaggccac ccccacgatg ggcagcgatc gcattgggct atcggcatat
     2761 tgccaggcca agaggcattg aaggaagagc gtcgttccaa tgatcgtggg tagtttgagc
     2821 agccacgcat agagcgggtc aagggagaag tggtgcatcg actcgaaaga caggaccaag
     2881 tcgacgctgg tcgcgcaaag agtccacagg agcggaggcc cgacgatcag accgacgaac
     2941 cacgatacac gtcgcagcgg gacccgaatc tgcggcgcca ggccaacccc caccgatcca
     3001 acttgaaggg caagaacgac ctggctgacg aggctcaccc ccgtccatgg cccggccaga
     3061 atctcgacgc ctgaagggag actcacggca acaccgtagc gccggtgggt tccaagaacc
     3121 gccgctagcc agaatcggtg ctgcggccgt gctgcacttt ctggtccgcc taacgttgcc
     3181 ttaagctgcg ggccgggctg gcgcgggcct tgcgcaagca aggaccgtga caggacggac
     3241 cgtcagcttc aaggcgttgt tatacagctc gtcggatcga acgatcatgc ccgggccttt
     3301 ccttgcgccg ctgcgggtcc aacgatcgtc gcaggactgt ccttgttcaa cgatccctcg
     3361 atcacgacgt cgccaacacg cttttccgac caacccgagt tcctcgactc gaaacggact
     3421 cgtcctcttt ccgggtcttg tataactaca aatggacggt tctggataac gccgttggtt
     3481 tcttgttcgg ctgctgccgt tgctgtgttt gctctgcttc atttgaggtg gtccgccgca
     3541 aggtgccgag ccgcatcggc ttggtcgtct tggcgttgcg ttcctcttga cattatacgg
     3601 aacctccgaa cggcgtagat ggtctctccc tcgggatcct cgggcccggc ggcatgcgga
     3661 ccggtttcgc gcagttccca ctgtgaaatc actgcggcgc cgcggcacct tcgcgttagt
     3721 atcggccggt accggtcgtg ggaccgctga acgcgggcgg ccgctcggga tcggatacgg
     3781 gggttgaact tgcacgacgt gtcggggccg ctctccgccg gagatgatgc gctgctgggc
     3841 cggctgccca ccgccacacc agggggcgag ccggcatgag tcacccgcgc gggtcgagca
     3901 agcagcgccg caagacgagc cgggcggcgg gcgagttgcg ggggaagctc cggccgcacg
     3961 atccgttcga gttgatccgc tggctcgcgc tgagccagcc cgatccgcgc aaggcgctcg
     4021 cggaactcgt gcagaacagt ctcgacgccg acgcgcacag gatccgcatt acgcgcaaac
     4081 gcgagcgcgg gacaccctgc ctacgtatcc tcgacgacgg cgccggtgtc atcccggagt
     4141 tgaatcgacc cgacgcactg aaatacatcg cgacgcacat cggtcacagc cgcaaacgct
     4201 ctctctcgcc gcaggagcgc ctgcagctga tgacgcaggg ccagtacggc atcggcctgc
     4261 tcggcttctg gagcctgggc gagctgctcg agattcgctc gtcggtcccg gaccaacgcc
     4321 cgcatcggtt ggtgctgcat cgcgaccggc cggactaccg gatcgagccc ctgcggggaa
     4381 acctcgccct cgacgagcgc tttaccgaag tcgtggtcgc ggggctgcac cgcgaagccc
     4441 tcggcgcgct gatcgggagg cgcgcggcgg actacctgaa gtccgagctg cgcgggcagt
     4501 tgctcgcgcg cgcggtcgat ctcgtcgtcg aggaccgcat gtcgcgcggc cgggcgcagc
     4561 ggatcctcac cgtcaaaccg cagcgcttcc tcggcgagcg cctggagggc ctggggccga
     4621 tcgacgtacc ggggcacgcc ccgatccggc tcgaggtcta cgtcgccgga gagggcggcg
     4681 acccggagaa cggacggctg tcgctctatt cggccgggac gctcgtcgcc gaggactttc
     4741 acgcgatggc accgctcggg ctcgaccgcc cgccgtggac cgatccgcgg ctgaccggca
     4801 tcgtcgactt cccgtcgttc cgggtcgcgc cgggaagccg gcgcggcgtc gtcctggacg
     4861 aagcggcggg cgcgttcgcc gccgcgctgt cggcagtcga caccgtgctg ctcggagtcc
     4921 tcgacgcgat cgaacggcgg cgggccgagg agctcgaccg gtcgatcgtg cgtgacctgc
     4981 agcgcgcctt ccgcgacttc caccgtcacc gcccgcgcta ctcgatgctg cccgtcgccg
     5041 agcgccccgg cccggcggcg ggaacctcgc cgggcaaagc cgacgacggc ggcggcggcg
     5101 cggtcgccgc cgagtctccc tcacccctcg cgccgtcgca gtcgagcgga cgatcgcctc
     5161 gcgaacccgc cgttgacctg ctgccgcccg ggccgctcga tggcgtgagg gtttccccgg
     5221 cgaacatgct cgtcgagtgc cgcggatcgc gcgaggcgcg agcgcaggcc gtcgacgcgg
     5281 tcggccgacc gatcgaagac acggtcgagt tccgctggct cgtggtgccc gcgatgctcg
     5341 gggccctgga ggtcggcacg ggtggatcga ctgcagaaag ccgcgcgcgc ttggccgccg
     5401 gcgagcacga gggcagcggc acgctcgagg tgacggcggt ctcgggcgag cgacgggcgc
     5461 aggcgacgat cggcatcgaa gtcgtcgacg agatccccgg cggccgcggg gacgagggga
     5521 tcccggaacc ggagctcgtc gatctgcccg gcgccggctg gcgctcccgg atgcacgacg
     5581 ggctgtggca ggtcaattct gggcaccgcg acttccgcgc cgcggcggcc agcccggtgt
     5641 tgaagctgcg ctacctcgcg atgctcttct cgaaagaggt cgtgctgcgc agccaccagg
     5701 acccgcggct cgaccagccg ctcgaacaac tggtcgaggt cgcggcgtac gccgaccgga
     5761 agctgctgcg caagggccgg cgaaagaagg gatgagcgct cagttgagca gctcggccgg
     5821 catcgcgcgg tcgccgagca gctcgcggaa gctcgccgcc ggcacggcgg ccgagaacag
     5881 gaagccctga tactggtcgc agcggcagtc gcgcaggaag gcgagctgtc ccgattgctc
     5941 gacgccttcc gcgacgacca ccagccgcag cttgtgcgcc atcgcgatca tcgccgaggt
     6001 gatcgccgcg tcgttctccg acgactcgac gccgcgcacg taggagcggt cgatcttgag
     6061 cgcgtcgagc gggaagcgcc tgaggtaggc gatcgccgag ttgccggtgc cgaagtcgtc
     6121 gacggacagt cgtaccccca tcgccttgag cgcgcgcatc tgctcgagca cggccgggtc
     6181 gctgcggagc gctccgcgtt cgctcagctc gagctcgagc aaccggccgt cgatgcccga
     6241 gtcgcggagg acgccgcgca cgacgtcggc caggtcgccg ctcaccagct ggcgggccga
     6301 gacgttgacc gacacgcgga tcggcggcag cccttcgtcg agccaggcgc gaagctgccg
     6361 gcaggcggtc gcgagcaccc aacgaccgat cgagtcgatc aggccggatt cttcggccag
     6421 cggcacgaac tcggccggcg acacgctgcc gagcttttcg ttgtgccagc gcaagagcgc
     6481 ctccgcaccg acgacccgcc cgctgccgag gttgaccagc ggctggtagt gcagctcgag
     6541 ctcaccgcgg tcgatcgcgc cacgcagatg gcgcccgacg agcaacctgc gctcgttgcc
     6601 gtcatcgacc gggccggaac agccgcgaac caagccgccg ccgtctcgct tggcggtttc
     6661 gagcgcggtc tcggcgcgat gcagcaaggt ctcggcatcg acgccgtcgg ccggcgagat
     6721 ggcgacgccg atgctggccg acacgaacag ctccttgcgg tcgacgacgt acgggtcgga
     6781 cagcgcgcga tgcagccgct tggcgatcgt catcgcgtgc tccgggcgcc tgaggccggc
     6841 gaacatcatc ttgaagtcgt cgccgctgaa gtgcgccagc gcggacggcg ggtcctccag
     6901 acggtcgctg ctgcgcaccg acccggtcag ccgaccggcg accagtcgca ggatgcgatc
     6961 acccgcgtcc cggccgtgtg cctggttgac gctacggaac cggtcgagcc cgatcgacat
     7021 caccgcgacg cggtcgctct tggcgaccgc ggctgccagg gtctgacgga aggccgcgtg
     7081 gttcggcaga cccgtcagca ggcccggcgg ttcgagacga ctgcgctgga tgcggtcgat
     7141 ccggtggctg cgatagagca ggtctgcgcg acggatcagc acgcgccagt cgtacggccg
     7201 ggtcacgacg tcggtgctcc ccgactcgag cgcggcgagt gcctggctcg taccggtgca
     7261 cagcgacagc acgggcaccc cgcgggcggc gggtagcccg cgcaatcgac cgcagaacgg
     7321 ctccgccgac gtgcggtcga gctcgtcgtc gaacaacacc aaaccgaacg cgcccgcctc
     7381 gacgcgccgc agcgcgtcat cgaccgtgcg cgcggtttcc acctggaaac cgacacgccc
     7441 gagccagcgc gtgccccagg tcaacgtcgc gccgtcgctc gagacgacga gcaccgggcg
     7501 gccggacccg ttttcgccgg cctccgcctc gctgtcgaaa cggtgtcctt ccatcggatc
     7561 ctccctcggg cacccgcgct gcgccgcgtc gggtccgagc tagcagcaac ggtgccaccg
     7621 gcccgcgacg ccgattcccg ccgtctggcc gcccgacggg cggggagtgc ggcggcgcga
     7681 ttcgaaaaag cggcgcaccg cctcgaaaaa gcggcccgcg cggttgtccg gcgcgcacgc
     7741 ggaccctaga ttcggccgag gcggtcgacc gctcctccgg gaccggatgt tcgcatcgga
     7801 tcaaccgctc agagcgtttt tgagccggct actgcgcacg agccgcgcgc gcgcggccac
     7861 gctctacctg ccgcccccgg ccgacctgca cggccgggcg ctgttggtgc acgagggcga
     7921 ggccgccccg ctacccgagc ttgccgaccc cgaggccgcc gagcagttct gtcgaggtct
     7981 gagcgatgcc aacctggccc atcgcgaccc ggtcgagagc ggcggccgtg gtggttggtt
     8041 gatcgcgctg cccgccctcg atgatctgct ctcggccgac tcggacggcg aggcgcgacg
     8101 gtctccgggc cgccgccgtc acgagtcgct ccatccggag agcggaccga cccagaccgc
     8161 ctggctcggc ctgcagttcg acgccgacac ctccgcgagc gaccggcttc cggcggcgtt
     8221 gtccgtcttg cgccgagatc gcgcgagcgg ctggcattgg ctcgtcgcgg tcggcggggt
     8281 gctggcctgg cacggccagc acgtctcgtc cctgttgcgc gagccggtca gccggttgcc
     8341 cggccgcacc tcgctccacg gcgtgctacg ggcgatgctc gccgccgccg cccgcgacga
     8401 ggcgcgcgtc gcgctgctgc tggtcaaccc ggacgccttc gccttggtca acgagcgttt
     8461 cggccgcgaa gcgggtgacg gcgtgatccg cgagatcgcc ggccggctcg agcgcgcgct
     8521 gcggcagacc gacttcgtct gccgctacgg cggcgccgtc ttcggcgtcg ccctgcccga
     8581 tgcgggcgaa cgcgagatcg agacggtcgc cgccaaactg cgcgatgcga tctgccgcga
     8641 cggctttctc gacggcgcgc tgcgcttgag caccagtttc ggcggggcga gccacgcgcc
     8701 cgatcgcgac gaggacctga cggaagcgat gctgcggctc gtgcgccgcg ccgaccgcgc
     8761 gctcaactcg gcgagcaagt cgggcggcgg gcggacggtg atctggcgcc gcgatcgtga
     8821 gctcgaggag atcggccagt tcgaccggct gagcgggatc ttcaccgcga acccgtcgaa
     8881 ggactaccgc aacatgatgc tgctgtggga caccgtccac gcggtggcct cgagcgacga
     8941 cgtcgcgcgg ctgatcgatc gggtcctcga gcggttggtg gccgcattca acgcggcacg
     9001 ggtgatgctg gtcgagcaca ccgacgccgg cgggtacgga ccggcgcggg cggcgtcgcg
     9061 cggcggtgag cgcggcgcgg cgacggaagg agagcgccgc ttcgccccgg atcacgagct
     9121 gcgggcgctg ctcgaccaag cgagcctcga gcgccgcgcg ctcgagcgac cgctgtcggc
     9181 cggacggctg ggttgcgccg tgccgctcgc ctcggacgat cgtcggctcg gcctgctcta
     9241 cctcgaaggc gacgcgcacg agttccagct cgactcgtcg gaccgcatcc tgctcggcgg
     9301 tctcgccgga caactggcga tcgcgctcga tcgagcgcgt ctggccgagg ccgaacggct
     9361 gcgtctcgac gacgagcgcc gtacgctgcg gcgcgagctg tccgagctgc gccgcgcgct
     9421 cggcgacgcc aagctggtgc accgctcgga agcgatggac gcggtgctcg acgtggcgcg
     9481 gcgcgtggcg ccgaccgacg cgaccgtgct gatctgcggt gagagcggca ccggcaagga
     9541 gctggtggcc aggacgatcc acgcattgag cgccagacgc gacggcccgc tggtcgtcgt
     9601 cgactgcagc gcgatcgcgg tgagtctgat cgacagcgag ctgttcggcc acgagaaggg
     9661 cgcctacacc ggcgccgagc gtggctcgac cggccggctg gccgaggccg acggcgggac
     9721 gatcctgctc gacgagatcg gcgagcttcc gctcgaggtg cagggcaagc tgctgcggtt
     9781 cgtccaggac cggcagttcg tcgccgtcgg cggaacgcgg ccgcggcgcg tcgacgtacg
     9841 actgatcgcc gcgaccaacc gcgatctcgc cgcggaggtc aagatcggcc gcttccgcga
     9901 ggacctgtac taccggctga tggtggtcca gctgaccgtc ccgccactgc gcgagcgacc
     9961 ggaggacgtc gtgttgctgg cacgccattt cgtcgacagc ttcgcgctgc agtacggccg
    10021 acagccgcga cgactgaccg ccgaagccga ggtgcacctc actgcccagg gctggccggg
    10081 caacgtgcgc gagcttcaga accgcgtgat gcaggccgtg atcctctcgc gccaggagcg
    10141 gatcggggtc gacgagctga cgacgggccg gccgacgccc gcccgtaccg cagccgatcg
    10201 ggcggcggga accgcggtcc ccgcgccggc cgaggcggcc gatggagacg catgggacac
    10261 gctacgcgcc gtgttgcgcc aacggatcgc gctcgcggcg aacgcggccg cgccgccgcc
    10321 gatcggccgc tgggtcgccg atgacctgat cctcgaggcg caccgccggt cgggccgcgt
    10381 cgcggcccat gccgcgcgcg ctctcggcat cgcgaagacg accttcaggc gacgtctgcg
    10441 caaggcggcg atcgaggccg agcgcggcac gagcccgagg ccgaacgact gggccgaggt
    10501 gctggcgtcg ctcgcgcgca tcgtcgccgc ggcgaacgac gacggcgagg accggacgcg
    10561 gcgggtccgc ctgctgctgc tcgaggaggt cgagcgacgg gtcacgcgcg acacgggatc
    10621 ggccgcgctc ttgatgggtg tgagcgatcc gacctacggc cgatgggtcg ccgagctggc
    10681 cggttgacac agaaagggaa gacgatgccg aagcaggttg gcgactacat cctcgactgt
    10741 ttgcgggcgc tcaacaaaga cgagggcgaa gtcgagctgg gcaaccggat ccacgacgta
    10801 cggcagtact ggcacgacct ttggggcggc gcggtgccgt ccggggcgcg catcgtcgcg
    10861 gcgttcctct cgaacgagcc gggttcccgc gagccgaagg acctgtggtt cttctcggac
    10921 tcccactgcc tgctcgcgca caacgtcatg tcggcccacc tcgacctgct ggtctacccg
    10981 ttccgcaacg gggtccatcg cttgcgcgtc gtgcgcaact acgacgatca gccgagcccc
    11041 ggctcgcgct tggtcgtcgg cctgtggttc gacgagcaga ccaaggtcgc gctcgaggcc
    11101 ggtgacggca actgcctgcc gctcgagacg ctgctcgagg aagtgctgat cccgcacctg
    11161 cagtagcgcg gtcggccggc ggcccggcgc gcgcgagcgt agtagaatcc gagtcacggg
    11221 agacgccgca cgcggccgcg tcggtcgcga cgagcggcga tcgagcgaga cgaagggcgc
    11281 ccattggacc gtgattccgg catcggccgg ttgcacgtga tcaccgacga gaccgttcaa
    11341 acacgctggt cgcacgtcga gttggcccgc gccgccgccc tgggcggcgc cgatcgcgtg
    11401 caataccgcg agaagcgtcc gcggacgacg cgcgagctgg tcgagaccgc gcggcggatc
    11461 cggcgcgcgc tcgacggcac ggcggcacgg ctcgtgctga acgaccggat cgacgtggcg
    11521 gtcgcggccg gggccggcgc ggtccacctg ggccgcgacg atctcgaccc ggcgatcgcc
    11581 cgacgtctcg tcggcgattc gatgcggatc ggagcgacgg ccaacgacct ggagcaggcg
    11641 ctgcgggcgg cgcggctgcc cgtcgactat ctcggcgtgg gtcccgtctt cggcacgcgt
    11701 agcaagaccg caccggcccc cgcgctcgga ttggtccggc tggccgagat cgtccgcgcc
    11761 gttccgcttc cggtgatcgc gatcggcaac atcaccgtcg accacgtcgc cgcggtgctg
    11821 gcgacggggg tccacggcat cgccgtgctg tcggcgatcg cctgcgccca cgatccgcgc
    11881 gaggcgacga gcgggttcgc ccgcgcgatc gaccatgccc gctcgggccc ggtggcgggg
    11941 tgagggacgg atgacgatcc gacggacgga cgtgacggtc gtcggcggcg gcgtgatcgg
    12001 gctgtcggtc gcggccgagc tgctgcgacg cggggtcgcg gtcgacgtga tcgatcgggg
    12061 tccgatcccg ggctacgcga ccgccgcggc ggccgggatg ctcgctccgg tttccgaagc
    12121 cgagctggaa gacgaagtcg tactgccgct ggcgctcgac agtctcgcgc gttttcccgc
    12181 cttcgtcgac tggctggaac gtacgaccgg tctgccgtgc gactaccgca ccgacggcac
    12241 gctgtgggtc gccgtcacgc gtgacgaccg ggcggagctc gagcacatcc atcacacgct
    12301 gacggaaaga atcggcggta tgccggcgcg ttggctgtcg gccggcgagg tccacgcgcg
    12361 cgagccccac ctgagcggcc gtgtgctctg tgggctgtgg gtcgagcgcg acctgcaggt
    12421 cgacccgcgc gcccttaccg cggcgctcga gcgggcgatc gtcgccgccg gcggaacgat
    12481 cgagcgcggc actcgcgtcg tcgggatcat cgccgacggc ggcgtggtgc gcgccgtcga
    12541 gctggcgacg cccgatggcg tcgcccggcg cgcggccggc ggcgtcgtgc tcgcggccgg
    12601 cgcctggagt ggccacgagc tgcggctgcc gctcggcgac ctcggcatcc gcccggtcaa
    12661 ggggcagctg atccgcttgg ccggcgcgcc gttgctgcgc cacgtggtgc gccacccgga
    12721 ggcgtatctc gtcccgcgcg gcacggggga gcttctcgtc ggtgccacga tggaggaggt
    12781 cggcttcgat ccggcggtga ccgccggcgc ggtccacgac atcctgcgca aggcgtggca
    12841 actgctcccc ggtctgtacg acctcgaact gcgcgaagtc tcggtcggct tccgcccgat
    12901 gtcgcgtgac aaccggccgg tgatcggccc gacggcgatc gccggtctct atctcgccgt
    12961 cggtcacggc cggcaagggg tgctgttggc accggcgacg gcgcagctgc tggccgatgc
    13021 gatcacaacg ggccgcgtgc ccgaagtgtt gctgccgttc gcgatcgacc gcttcggcgg
    13081 ggcgaccgcg acggtcgggg gatgaccggg atggacgatt tcgatttcct cgtcggcagg
    13141 aactggcacg tcaggaaccg agtgcgggtc gagcgactgg cgaactgcga cgagtggacg
    13201 gagttcgacg cacggctgct cgacgtacgc aagatcctcg gcggcctggg caacatcgac
    13261 cgcttctcgg cccgccgcga cggcaagccg ttcgaggcgg tcagtctcag gctgttcaac
    13321 ccgtcgtccg ggttgtggac gatctactgg gcggacaacg tctcggcgac gctgaccgag
    13381 caggtcaccg gtcgcttcgt cgacggcgtc ggcgagttcc tcggccgcga gacctaccgg
    13441 ggccagacgg tcgagctgcg cttccgttgg accgacgtga ccgagcgctc gccccgctgg
    13501 gagcaggcct atctcgacga gtcgaccggc gagtgggaga cgaactggat catgcggttc
    13561 accgaactcg aaactcgatg agtcctactc ctgctcgtcg cccgccgccg tcagctcctc
    13621 gagctgtgtc ttggcccatg cgttctccgg gtcgagctcc aacgcctcgc ggaaggcggc
    13681 gatcgcctgc tcccgctcgt cgcgcatgac gtgcaggcgg ccgaggagca gccgcgcgta
    13741 ggcctcggtc gggttcagct cgacgttggc cgccgcgatc gccaacgcat cgtccaacct
    13801 ctgctcgcgg gccagcttct cgacgatcca gttgagcggc ccctgtgaga agtcgtaggc
    13861 ggcgctgccg aagtgctgct cgcgcagctc gcggtacttg gcgagcgccg caccgacgcc
    13921 gttcgcctcg atctccgcgt cgaggatctc ctggatcggc ttcggtacgg tcagcccgcg
    13981 atggcaggtc acacaccgca cccgcgtcgt ctccgcgcgg ccggtctcga tcgccttcag
    14041 atgcctgccg ttgatctcat cgaccatctt catcatggtg cgggccgtac gcttcaccgc
    14101 cttctcgtcg ctggcgaagt ccatcccttg gagatcgtcg ggaccgacgt gacagtaggc
    14161 gcagcgcacc cccaggctcg aggcgacctc gcgcatcaaa ccgacgagct cgctcttcgt
    14221 gatctccttc ggcacgaccg tcagattcgt gtactcgtcg gggatctgcg ctccggcgtc
    14281 gcgcgcaccc gcgatcagca gacaagcgag aacggagcac cggaccaccg cgcgcgtgtg
    14341 gatgggtctc atcggtcagc ctccctcggc atgccgccga tcgtaccggg tgcggctcgc
    14401 cgggaggtga acgaacggca aggaagcggt cagcgttcgt tcaggagaat gtcaggaaac
    14461 ggtcaactcc gctcgcgcgg cgggctgcgg gcgagagccg ccgcgcgccg ctgctagaat
    14521 cgccggaatg agaggaaccg tgcgcgcgct tcgccgccgc tggcgctcgg ccgtgaccgt
    14581 cgcgctcctc tccgggacac tcgtgatcgt cgccgtgctg acgctccaag cgcagtttgc
    14641 ctggacctac caccggacga ccgccgagaa cgtgctgcgg gattactcgc gcctggtcgc
    14701 cgaggaattc atccgccgga cgagccagga gatcggctac cgcggcttct tcagtgtcgt
    14761 gaccgcgctg cagcagagcc tcggcggcgt ggccgcgccg gtgctgatcg agctcgccga
    14821 gattccgggc ggcctcgacg acgcccgcgc tccccgcgag ctcgtccgga cgctgtttct
    14881 cgtcgatccg gagaccggtt ccgtcgagac caagggcgtg cccttggacg cggagacggc
    14941 ggccgtgctc cggcagcgga tcggttcgtc tctcccggcg agcggcgaac gggccggacc
    15001 gttctcggca atccagctgg gcgacgaagc cggccagcgg atcgtgatcg tggtgccgat
    15061 cgccgcgccg gccggcagct ccgcgcggct cgccggtttc gaggtcgaca ccgcgggtct
    15121 ttcgacctgg ctgcggcgga tggtcgactc acgaccgctg cttcccgcgt cactcggtga
    15181 cggcgagctg acgaacgacc tgctcttcct ccggctgtcg gacgccacgg gccgggtcat
    15241 cttcacctcg ggcacgtggc cgggtccgct gctcgccacc gaaacgccga tgggcgacgc
    15301 gtacggcggc gtcttcgacg ggctgacgct caccgtctgc atcgacccgg cggccgcgcc
    15361 ccggctcgtc atcggcgggc tgccccgctc gcgcctgccc gtgctgtcgg gactgctgct
    15421 gctcaccgcc ggactcctcg gggcggcggt ctggcagatg cgccgcgagc gggcgctggc
    15481 ccgcatgcgc tccgagttcg tcgggcgcgt ctcgcacgag ctgcgcacgc ccttgacgca
    15541 gatccgcatg ttcgccgaga cgctgtcgct cggccgcacc cggacgcccg cggagcaccg
    15601 ccgctcggtc gagatcatcg atcaggaggc gcgacgcctc ggccatctcg tcgagaacat
    15661 cctccagttc tcccgggagg agcgtggcgc gacgtgcctc gcgccgcgcc cgttggagct
    15721 ggcacccttg gtgcgtgacg tgatcgagaa gttccagccg gtcgcgagcg cgcggcgggt
    15781 gcgcctggcg acggacctgg tggaggacgc gatggtcctc gccgacgacg aggcgatccg
    15841 ccaagtcttc ctcaacctgc tcgacaacgc ggtcaagtac ggtcccgcgg ggcaggaggt
    15901 tcgggtcggc ctcgaacgaa ccgggcggat ggtgcgcgcc accgtcgagg acgagggtcc
    15961 gggcgtgccc gagcgcgatc gagagcggat ctgggcccgc tggcagcgtc tcgatcgcga
    16021 cgagcgccgg gcgatctcgg ggacgggaat cggtctgacc gtcgtgcggg agctggtgtc
    16081 gcttcacggc gggcggacct gggtcgagcc cggccgacgc cgcggcgcgc ggttcgtcgt
    16141 cgagctgccc gtcacggccg gaggtacggc gtgaagcgga tcctgatcat cgaagacaac
    16201 gccgatctcg ccttcggtct gaggaacaac ttcgagctcg agggctacga ggtgcgcgtc
    16261 gcgggcgacg ggccggccgg cctcgccgcg ctcgcggacg cggaagccga tctggtggtg
    16321 ctcgacctca tgctccccga gatgaacggc ttcgacgtgc tcgagcagat ccggcgtcgc
    16381 ggctcggtcg tgccggtgct gatcctgacc gcgcgcgggg aggaatcgga caaggtgacg
    16441 ggtctgcgtc tcggggccga cgactacctg acgaagccgt tcggcctgat ggagttgctg
    16501 gcgcgggtgg aggcgctgct gcggcggtcg accgcgaccg gcgagggccg ctcgagaacg
    16561 aaggcgatcc tcaagttcgg tgcgatcgaa gtcgatccgg cgacgcgcac ggtcacccgc
    16621 gacgccgcgg tcgtccacct cgcgcccaag gagttcgatc tgctcgtggc gctgatccgc
    16681 cacgacggcg cggtcgtttc gcgggaagag ctgctcgacg aggtctgggg ttatgcgagc
    16741 gacgtcgcgt cccgcacggt cgacacgcac atcgccgagc ttcgccggaa gctcgagcac
    16801 gacccgtcga tgccgcgcca cctcctcacc gtccgcaagg ccggctaccg cctggcgcgc
    16861 tgacggctcg ccggggttga caggacccgt atactcggac cggttcgccg ggacggggcg
    16921 gatcccaaca cgacagagga gagcatcatg gttcgtagcg cgagtgcggt ttggcagggc
    16981 gatctgaaag gcgggagcgg ccggttgtcg gcgccgagcg gcgtg
//
LOCUS       DASPGN010000325         2739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1580325, whole genome shotgun sequence.
ACCESSION   DASPGN010000325 DASPGN010000000
VERSION     DASPGN010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580325"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="VD788_09490"
     CDS             complement(<1..1610)
                     /locus_tag="VD788_09490"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV76540.1"
                     /translation="MTAARHYEREGGLSRFSLDRRITVLVLFLTLLVVGAAAATRIPV
                     ELIPAGYDNPFLQVVVPWEDAPPKEVMDKVVLPLEEELSTVRGLDRLTTLSVTGRGMA
                     WLSFKHGTDMNVAYREVRDRIERARNRFPEDIDRTYIRKEDASGIPVHVMGVAIDESV
                     ADPYNLIQNEVILPLERIDGVASVTADGLIEKEVLIELDREKTNAAGLNIYDLSVELG
                     ADNFTMASGTVLDGSRKLLLRSVARVNTLEELQDRLVGESVRLGEIATISYEQPERDF
                     RVRAMSKPAVAVLVLKEGDANVREVSRKVAAVAERLSDNPRLQLLETITIFNQGEVID
                     EALSTLLESGLIGGLIAVCVLFLFLRRLRLTLIIALGIPLSLLIGLTVMYFTGESLNL
                     LTLLGLMLCVGLLVDNSVVVAENVHRLHREGLERREACIHGAGEVGLAITMSTLTTIV
                     VFLPVALVEGPAQFFLLRLALPVCVSVGASLLVALVFIPLCVYLTLPANGGNAHGGLT
                     RLAHERLNRVLRWVYDASFGRLNHAYNKL"
     gene            complement(1607..>2739)
                     /locus_tag="VD788_09495"
     CDS             complement(1607..>2739)
                     /locus_tag="VD788_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV76541.1"
                     /translation="IEVEVHGDDLGRLRDYSDRVTAELARVPELADVEATLESGAPEV
                     QIVYDRDRLAQYGLNIRDVARMVRDKVQGYEATRFNMKDRRIPIVVRLNLDDRETVDD
                     VRSIIINPGADRPIRLASVADVTLGEGPSEVRRVDGRRVALINANIAAGALSVATERI
                     AETLDRRIDWPADMTYFIAGQNEEWEASRNSLWIALALSVFLVYVIMAAQFESLIHPL
                     VIMLTIPLAFVGTVVALKLLGLALSIVVFLGMIMLAGIVVNNAIVLVDYINTLRRRGI
                     ERDEAIVTAGKVRLRPILMTTATTVLGLVPMAIGLGDGAEIRTPMAIAVISGLISSTA
                     LTLIVVPTCYALADSMTSRLFGRQTVRVDAPDAVPGGSEAMT"
BASE COUNT          509 a          952 c          830 g          448 t
ORIGIN      
        1 agcttgttgt aggcgtggtt caagcgcccg aacgacgcgt cgtagaccca tcgcagcacc
       61 cggttcagcc gttcgtgggc cagccgggtc agcccgccgt gggcgttgcc gccgttggcc
      121 ggcagggtga ggtagacgca caacgggatg aaaaccagcg ccacgagcag cgatgcgccg
      181 accgagacgc acaccggcag cgccagtcgc agcaggaaga actgggccgg gccttcgacc
      241 agcgccaccg gcagaaagac gacgatcgtg gtcaacgtcg acatcgtgat cgccaggccg
      301 acctcgcccg cgccgtggat gcaggcctcg cggcgctcca acccttcgcg atgaagtcga
      361 tggacgttct ccgccacgac aaccgagttg tcgaccagca gtccgacgca gagcatcagt
      421 cccaacaacg tcaacagatt gagactttct ccggtgaagt acatcaccgt cagcccgatc
      481 aacaggctga gcgggatccc gagcgcgatg atcagcgtca accgcaagcg acgcaggaac
      541 aggaacaaga cacagaccgc gatcaacccg ccgatcaagc cggactcgag cagcgtgctc
      601 aacgcctcgt cgatcacctc gccctgattg aagatcgtga tcgtctcgag caactgcaat
      661 cgcggattgt cggacaggcg ctcggcgacc gccgccacct tgcgcgacac ctcgcgcacg
      721 ttggcgtcgc cttccttcag caccagcacc gccaccgccg gcttgctcat cgcccgcacg
      781 cggaaatccc gctccggctg ctcgtaggag atcgtcgcga tctcccccag ccgaacggat
      841 tcgccgacca ggcggtcttg cagctcctcc aacgtgttga cccgcgccac cgaccgcagc
      901 agcagcttgc gcgaaccatc gagcaccgtt ccgctggcca tcgtgaaatt gtcggctccc
      961 agctcgaccg acagatcgta gatattcaac ccggcggcgt tggtcttctc gcggtcgagc
     1021 tcgatcaaca cttccttctc gatcaatccg tccgcggtga ccgatgccac accgtcgatc
     1081 cgctcgagcg gcagaatgac ttcgttctgg atcaggttgt acgggtcggc caccgattcg
     1141 tcgatcgcca cgcccatcac gtgcaccggg atccccgacg catcctcctt gcggatgtag
     1201 gtgcgatcga tatcctccgg aaagcgattc cgggcgcgct cgatgcgatc gcgcacctcg
     1261 cgataggcga cgttcatgtc ggtgccgtgc ttgaagctga gccaggccat cccacggccg
     1321 gtcaccgaaa gcgtggtgag acgatcgaga ccgcgtaccg tgctgagctc ctcctcgagc
     1381 ggcagcacga ccttgtccat cacctccttc ggcggcgcgt cctcccacgg gacgaccacc
     1441 tgcaggaagg ggttgtcgta gcccgctgga atcagctcga cggggatgcg ggtcgccgcg
     1501 gccgccccga cgaccaacag cgtgaggaac aggaccagca cggtgatgcg gcggtcgagc
     1561 gagaaccggc tcagaccgcc ctcccgctcg tagtgccgcg ccgccgtcac gtcatcgcct
     1621 ccgaccctcc cggaacggcg tccggcgcgt cgacccgcac ggtctggcgc ccgaacagac
     1681 gcgaggtcat cgaatcggcc agggcgtagc aggtcggcac tacgatcagc gtcagtgccg
     1741 tcgagctgat caggccgctg atcaccgcga tcgccatcgg ggtgcggatc tccgccccat
     1801 ctcccagccc gatcgccatc ggtaccaagc cgagcaccgt cgtcgccgtc gtcatcagga
     1861 tcggtcgcag gcgcacttta cctgccgtta cgatcgcttc atcgcgctcg atgccgcggc
     1921 gacgaagggt gttgatgtag tcgaccagca cgatcgcgtt gttgacgacg atcccggcca
     1981 gcatgatcat tcccaagaag acaacgatcg acagcgcgag cccgagcagc ttgagcgcga
     2041 ccaccgttcc gacaaacgcc agcgggatcg tcagcatgat caccagcgga tggatcaacg
     2101 actcgaactg cgcggccatg atgacgtaga ccaggaagac gctgagagcc agcgcgatcc
     2161 acaagctatt gcgactggcc tcccattcct cgttctggcc ggcgatgaag taggtcatgt
     2221 cggccggcca gtcgatgcgt cggtcgaggg tctcggcgat gcgctcggtg gcaacgctga
     2281 gcgctcccgc ggcgatattg gcgttgatca atgccactcg acgaccgtcg acacggcgca
     2341 cctcgctcgg cccctcgccc aacgtgacgt cggccaccga cgccaaccgt atcggacggt
     2401 cggcgccggg gttgatgatg atcgaacgca cgtcatcgac cgtttcccgg tcgtcgaggt
     2461 tcagtcggac gacgatcgga atgcggcggt ctttcatgtt gaaccgcgtc gcctcgtagc
     2521 cctggacctt gtcgcgaacc atacgcgcca cgtcgcggat gttgaggccg tattgggcca
     2581 gccgatcgcg gtcgtagacg atctgcacct cgggcgcacc gctctcgagc gtcgcctcga
     2641 cgtcggccag ctccggcact cgtgccagtt ccgccgtcac ccgatcggag tagtcgcgga
     2701 gccggcccag gtcgtcgccg tggacctcga cctcgatcg
//
LOCUS       DASPGN010000326         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1228786, whole genome shotgun sequence.
ACCESSION   DASPGN010000326 DASPGN010000000
VERSION     DASPGN010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228786"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VD788_09500"
     CDS             <1..459
                     /locus_tag="VD788_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76542.1"
                     /translation="MLGHGRAQAIALHLEYDWRPESNFARAALADRVLPQLERGPQLA
                     VELARTAGDRDRWEIAWNVSQTSFDLPQIAAYVESWLKDDVGWRLISSDASADRSSAR
                     WSFEDGETARWQATSGIETADTDPRRYRVTLSIERSPGPAAGRAAVAGGG"
     gene            complement(834..2945)
                     /gene="glgX"
                     /locus_tag="VD788_09505"
     CDS             complement(834..2945)
                     /gene="glgX"
                     /locus_tag="VD788_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HXV76543.1"
                     /translation="MRLCPGSPFPLGAGWDGSGINFAIYSEEAERVELCLFDEIESRR
                     ERQSIPLPERTGHVWHGYLPDGRRGQLYGYRVFGPYQPREGLRFNPNKVLVDPYAKAV
                     GRTTRWDDSMFGYRVGHPAADLSFDTRDNAAFAPLASVLDPAFDWGDDRAPNTPWSRT
                     IIYETHVRGLTWRHPALPPELRGTYAGLASEPVLEHLERLGVTAVELMPIHHHVDDRR
                     LVERGLVNYWGYNTLSYFAPDARYAGRGGAAHEFKTMVRTLHRAGVEVILDVVYNHTA
                     EGDRMGPTLSLRGVDNRTYYRLKPNDRRRYLDFTGTGNTLNVQHPQVLRMIADSLRYW
                     IVEMHVDGFRFDLASALARESYDVDKLGSFFDIIYQDPVISRVKLIAEPWDLGRGGYQ
                     VGQFPVSWSEWNGKYRDAVRAFWRDRSSSLGELATRVAGSSDLYRPGGRSPRASINFV
                     TSHDGFTLADLVSYENKHNLANGEENRDGDPHNLSKNFGVEGPADDPAIRALRRRQRR
                     GLLATLLFSLGVPMISGGDELSRTQRGNNNAYCQDNETSWYDWELDDDRRRFLEFVRA
                     AIAVRNAQPVFRRRRFLADGDVRWLNPDASAMTGEDWSAPGRGHVGLLLSASAIDETD
                     ETGRVAEGDSMLLLINAEERPVTYALPPHDGLSGWQLVLDTWREQPETETERDRGAGE
                     AYPLRPRTVALFRGVGGAATA"
BASE COUNT          447 a         1045 c         1067 g          454 t
ORIGIN      
        1 atgctggggc acggccgggc gcaggcgatc gcgctccatc tcgagtacga ctggcggccg
       61 gagtcgaact tcgcccgcgc cgcgctcgcc gaccgcgtgc tgccacagct ggagcgcggt
      121 ccgcagctgg ccgtcgagct cgcccgcacc gcgggtgacc gcgatcgctg ggagatcgcg
      181 tggaacgtca gccagacgag cttcgacttg ccgcagatcg cggcctacgt cgagagctgg
      241 ctgaaggacg acgtcggctg gcgcctgatc tcgagcgacg cgtcggccga ccggtcgtcc
      301 gcgcgctgga gcttcgagga cggcgagacc gcccgctggc aggcgacgag cgggatcgaa
      361 acggccgaca ccgatccgcg gcgctaccgc gttacgctgt cgatcgagcg atcgccggga
      421 ccggcggccg gccgcgcggc cgtcgccggc ggtggatgag ccgcggccgc gctcggtcgg
      481 acccgcccag cggctcgtcg gccaccagga tgagcgccgc cagcggcggg agtctcagcc
      541 gcagcgcgcc gccggtcggc gtcccagcga acgaagctga ggaagctgtg gcggctgtcg
      601 tgacagtcga tccactcgaa gccggccggc tcgaagtcgc gctggtgcag cgcgcgttca
      661 tcgcgcagcg gggtgttgag gctgccagtc caggccgcgg tcgtgatccc actcgcgcca
      721 ctggccgaac tcgtcgccga tgaacatcgc cgatgaccga gaccgatctc gcggcggggg
      781 cccaaacgcc aggtcgtcct cgctcagccg gccaccggac cgtcggccgg agctcacgcc
      841 gtcgctgcgc cgccgacgcc gcggaacagg gcgacggtgc gcggccgcag cgggtaggcc
      901 tcgccggcgc cgcggtcgcg ctcggtttcg gtctccggct gctcgcgcca ggtgtcgagc
      961 acgagctgcc agccggaaag cccgtcatgc ggcggcagcg cgtaggtcac cggacgctcc
     1021 tcggcgttga tcagcagcag catcgaatcg ccctccgcca cccgtcccgt ctcgtcggtc
     1081 tcgtcgatcg cgctcgccga cagcagcagc ccgacgtggc cgcggccggg agcgctccag
     1141 tcctcgccgg tcatcgcgct cgcgtccggg ttgagccacc ggacgtcgcc gtccgcgaga
     1201 aaccggcgcc gtctgaacac cggctgggcg ttgcgcacgg cgatcgccgc acgcacgaac
     1261 tcgagaaaac ggcgtcgatc gtcgtcgagc tcccaatcgt accaactggt ctcgttgtcc
     1321 tggcagtagg cgttgttgtt gccgcgctgc gtgcgggaca gctcgtcgcc gcccgagatc
     1381 atcggcacgc ccagcgagaa cagcagcgtc gccagcaggc cacgtcgttg tcgtcgcctc
     1441 aacgcgcgga tcgcggggtc gtcggccggc ccttcgacgc cgaagttctt gctgagattg
     1501 tgcggatcgc cgtcgcggtt ctcctctccg ttcgccaggt tgtgcttgtt ctcgtaactg
     1561 acgaggtcgg ccagggtgaa gccgtcgtgc gacgtgacga agttgatgct ggcgcgcggg
     1621 ctgcggccgc cgggccggta caggtcgctg ctgccggcga cgcgcgtcgc gagctcgccg
     1681 agcgacgacg accggtcgcg ccagaaggcg cggacggcgt ctcggtactt cccgttccac
     1741 tccgaccacg acacgggaaa ctgtccgacc tggtagccgc cgcgccccag atcccacggt
     1801 tctgcgatca gcttgacccg cgagatcacc gggtcctggt agatgatgtc gaagaacgag
     1861 ccgagcttgt cgacgtcgta cgactcgcgg gccagcgcgc tcgccagatc gaaccggaac
     1921 ccgtcgacgt gcatctcgac gatccagtag cgcaggctgt cggcgatcat gcgcagcacc
     1981 tggggatgct gcacgttcag cgtgtttccg gtgccggtga agtccaggta gcgccgacgg
     2041 tcgttcggct tcaaccgata gtaggtccga ttgtccactc cgcgcagcga cagggtcgga
     2101 cccatgcggt cgccttcggc ggtgtggttg tagacgacgt cgagaatcac ctcgacgccg
     2161 gcccgatgca gcgttcgcac catcgtcttg aactcgtgcg cggcaccgcc ccgaccggcg
     2221 tagcgcgcgt cgggagcgaa gtaggacagc gtgttgtaac cccagtagtt gacgagccct
     2281 cgttcgacga ggcgccggtc gtcgacatgg tggtggatcg gcatcaactc gacggcggtg
     2341 accccgagtc gctcgaggtg ctcgagcacc ggctcgctcg ccagtccggc gtaggtcccg
     2401 cgcagctcgg gcggcagcgc cgggtgacgc caggtcaggc cccggacgtg ggtctcgtag
     2461 atgatcgtgc ggctccacgg cgtgttcggc gcccggtcgt cgccccagtc gaaggcgggg
     2521 tcgagcaccg aggccagcgg agcgaaggcg gcgttgtcac gcgtgtcgaa cgacagatcg
     2581 gcggccgggt ggccgacgcg gtagccgaac atcgaatcgt cccaccgcgt ggtgcgcccg
     2641 acggccttgg cgtacggatc gacgagcacc ttgttcggat tgaagcgcag cccctcgcgc
     2701 ggctggtacg ggccgaacac gcgatagccg tacagttggc cgcgccgccc gtccggcagg
     2761 tagccgtgcc agacatggcc ggtgcgctcg ggcagcggga tcgactggcg ctcgcgccgc
     2821 gactcgatct cgtcgaacag acacagctcg acgcgctcgg cctcttcgga gtagatcgcg
     2881 aagttgatgc ccgacccgtc ccagccggca cccagtggaa acgggctccc cgggcagagt
     2941 ctcatcgggt tcgcccgagc acgcggtagc tttcgggagc ggcgtagagg aacagcagcc
     3001 cgtcgcgacg ctc
//
LOCUS       DASPGN010000327         2332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_409651, whole genome shotgun sequence.
ACCESSION   DASPGN010000327 DASPGN010000000
VERSION     DASPGN010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409651"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VD788_09510"
     CDS             complement(<1..1395)
                     /locus_tag="VD788_09510"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXV76544.1"
                     /translation="MTRFVFVTGGVVSSLGKGLASASLGAILEARGLKVRMVKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGTETDLDLGHYERFVRTTTGRNSNFTTGRIYERVIARE
                     RRGDYLGATVQVIPHITDEIIRSIRLGAGDADVCMVEIGGTVGDIESLPFLEAIRQLG
                     VELGRNNCVYMHLTLVPIVGGSGEIKTKPTQHSVKELRGIGIQPDVLLCRCRDPLPPE
                     QRRKIALFTNVEERAVISAVDADDIYKIPILLHEQQLDGIVCDKLRIDAPPADLREWQ
                     QVVAGRQQHDATVNIAMVGKYVQIRDSYISLNEALRHAGLKTRTRVQVHYVESTDLES
                     AGTASLAGMDAILVPGGFGERGIEGKVAAVRHAREHGVPYLGICLGMQVAVIEFARGV
                     LGLPGANSTEFDREAPDPVIALITEWRDRSGETLKRDDRSDLGGTMRLGAQESRILTG
                     TLARKVYGRDTIHER"
     gene            1600..2220
                     /locus_tag="VD788_09515"
     CDS             1600..2220
                     /locus_tag="VD788_09515"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXV76545.1"
                     /translation="MKRAQAGFTLLELMVVVAIVGILMAVAIPAMGDFIRNGRITAAA
                     NDVMAALHFTRSEAIKWRQPVTLCTSANALQANNQPNAAAGCADSPLLTGWIVFVDLN
                     QDGVRDAGDTLLLNHGPMDARITAASEVDPFLVTYLMNGFALNTNGARIALCDQRGNT
                     PSGGELSSARAIFVAVTGRAGVSRDMDEIQTLVDEIGADVGGCEAS"
BASE COUNT          344 a          807 c          798 g          383 t
ORIGIN      
        1 gcgctcgtgg atcgtgtcgc ggccgtagac cttgcgcgcc aacgtgccgg tcaggatgcg
       61 ggactcctgc gcgccgaggc gcatcgttcc gccgaggtcc gagcggtcgt cgcgcttcag
      121 cgtctcgccg ctgcggtcgc gccactcggt gatcagcgcg atgaccgggt ccggcgcttc
      181 ccggtcgaac tcggtggagt tcgcacccgg cagtccgagc acgccgcgcg cgaactcgat
      241 caccgcgacc tgcatgccga ggcagatgcc gaggtagggt acgccgtgct cgcgcgcgtg
      301 tcgcaccgcc gcgaccttgc cctcgatgcc gcgctcgccg aaaccgcccg gcaccaggat
      361 cgcgtccatg ccggcgaggc tcgccgtgcc cgcgctctcg aggtcggtcg actcgacgta
      421 gtgcacctgc acgcgcgtgc gcgtcttgag cccggcgtgg cgcagcgcct cgttgaggga
      481 aatgtaggaa tcgcggatct gcacgtactt gccgaccatc gcgatgttga ccgtcgcgtc
      541 gtgctgctgc cggccggcga ccacctgctg ccattcgcgc aggtccgcgg gcggggcgtc
      601 gatcctgagc ttgtcgcaca cgatcccgtc gagctgctgc tcgtgcagga gtatcgggat
      661 cttgtagatg tcgtcggcgt ctaccgccga gatcacggcg cgctcctcga cgttcgtgaa
      721 cagcgcgatc ttgcggcgct gctccggcgg gagcgggtcc cggcagcggc acagcagcac
      781 gtccggctgg atgccgatgc cgcgcagctc cttgaccgag tgctgggtcg gcttggtctt
      841 gatttcgccc gagccgccga cgatcggcac cagcgtcagg tgcatgtaga cgcagttgtt
      901 gcggccgagc tcgacgccga gctggcggat ggcctcgagg aacggcagcg actcgatgtc
      961 gccgaccgtg ccgccgatct cgaccatgca gacgtcggcg tcgccggcgc cgaggcggat
     1021 gctgcggatg atctcgtcgg tgatgtgcgg gatcacctgg accgtcgcgc cgaggtagtc
     1081 gccgcggcgc tcgcgtgcga tcacgcgttc gtagatacgc ccggtggtga agttgctgtt
     1141 gcggcccgtg gtcgtgcgca cgaagcgctc gtagtgcccg aggtcgaggt cggtctcggt
     1201 gccgtcctcg gtgacgaaga cctcgccgtg ctggaacggg ctcatcgtgc ccgggtccac
     1261 gttgatgtac gggtcgagct tgaccatgcg gaccttgagg ccgcgggcct ccaggatcgc
     1321 gccgaggctg gccgaggcga gccccttccc cagggaggag accacgccgc cggtcacgaa
     1381 cacgaatcgc gtcatcggaa tccccgtcgt ccgcccgaaa cgacgggaga tcagtctagt
     1441 cgagcggtgc gggacccgcc agaggaaatg tgaagcgcgt cacgcgcgag ccgacataag
     1501 cagacgcgga ttccggcgtc cgggccgttc gtcgccgccg cgcgagcgcc cgtcggaacg
     1561 cgcgaatcgg cccttcccgc cctccttata gtgcaaccca tgaaaagggc acaggccggc
     1621 ttcacgctcc tcgagctcat ggtcgtcgtc gcgatcgtgg gcatcctgat ggccgtcgcc
     1681 attccggcga tgggcgattt catccgcaac gggcgcatca ccgcggccgc gaacgacgtc
     1741 atggcggcgc tgcacttcac gcgctccgag gcgatcaagt ggcgccagcc ggtcacgctg
     1801 tgcacgagcg ccaacgcgct ccaggccaac aaccagccga atgccgccgc cggctgcgcg
     1861 gattccccgc tcctgaccgg ctggatcgtg ttcgtcgacc tcaaccagga cggcgtgcgc
     1921 gatgccggcg acaccctcct gctgaaccac ggcccgatgg acgcgcggat caccgcggcc
     1981 tccgaagtcg atccgttcct cgtgacctac ctcatgaacg ggttcgccct caacaccaac
     2041 ggcgcgcgaa tcgctctctg cgaccagcgc gggaacacgc cgtcgggcgg cgagctgtca
     2101 tcggccaggg ccatcttcgt cgccgtgacc ggccgggccg gcgtctcgcg cgacatggac
     2161 gagatccaga ccctggtcga cgagatcggg gccgacgtcg gcggttgcga ggcgagctga
     2221 gtcatgacgc tcacgaacag gcaatccggg ttctccatgg tcgaagtgct ggtcgccctc
     2281 gtcgtgctgg cgatcggcct gctgggcatc gccgcgctgt acctcaacag cc
//
LOCUS       DASPGN010000328         3808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_58268, whole genome shotgun sequence.
ACCESSION   DASPGN010000328 DASPGN010000000
VERSION     DASPGN010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58268"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..2182
                     /locus_tag="VD788_09520"
     CDS             308..2182
                     /locus_tag="VD788_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXV76546.1"
                     /translation="MTIEAPPKSPKAKPQRLERAVIRFAGDSGDGMQLTGMQFTAATA
                     AAGNDLATLPDFPAEIRAPAGTMAGVSGFQIQFASDTVFTPGDRPDVLVAMNPAALRA
                     NLADLAPGGILIVNVDSFKDNDLRKAGYERNPLEDGSLDGYQVFKTELTRLTRAALKE
                     VDLPTRSADRCKNFFALGMMYYLYHRPMTVTLAWIERKFAGKELLIEANRLALKAGYA
                     YCEATEQFASSYEVPAAPLEPGTYRNISGNQALALGFVAAARRAGLTLFQGSYPITPA
                     SDLLHELSTFKNHGVITFQAEDEIAAIGAAIGASFAGSLGITSTSGPGLALKSEFLSL
                     AIMAELPLVVVDVQRAGPSTGMPTKTEQADLLMALYGRFGESPCIVLAPSTPADCFNA
                     AYEACRLSLLHMTPAILLSDGFIANGAEPWKLPEPDELPPIPVAFHRQVDGFQPYRRD
                     PHTLSRPWAVPGTAGLEHRIGGLEKAEGSGNVSYDPANHEAMVKLRQAKVDRVADDIP
                     PAEVTGDADGELLVVGWGSTYGAITSAVRRATQAGLRVAQLHLRHLNPLPSNLGEILD
                     RFPRVLVAEINMGQLAFVLKGRFVKPVLSLTKIQGTPFAASEVVAEIERLLGTRANDH
                     "
     gene            2172..3185
                     /locus_tag="VD788_09525"
     CDS             2172..3185
                     /locus_tag="VD788_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXV76547.1"
                     /translation="MTTEIKLSKKDFTSDQEVRWCPGCGDYAILSAAQQVFPELGVPR
                     EKFVIVSGIGCSSRFPYYMKTYGFHTIHGRAPSVATGVKLANPELSVWIVTGDGDGLS
                     IGGNHIIHLFRRNVDVNVMLFDNRIYGLTKGQYSPTSETGKRTKSTPFGSVDRPFNPC
                     ALALGAGATFVARSVDIYADHLRQTLKTAHAHRGTSFIQIFQNCNIFNDGAFEQIREK
                     GLREEHLLLLEHGKPLVFGKDLDRGIRLSGCNRPEIVELGNGVGESDLMVHDEAAPIG
                     YAAMLSEMQPPSWPMPVGVIRRIDAPCFDAAVHRQIADVTADRGNGDLRAAIHSGNTW
                     KVS"
     gene            complement(3178..>3808)
                     /locus_tag="VD788_09530"
     CDS             complement(3178..>3808)
                     /locus_tag="VD788_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76548.1"
                     /translation="LGIEAGQHDAPGSVDRSEAILWIALVAAGAIDARATPDINRHRR
                     FLREQSRGIPRVIEVRHRHALRATDGFRMGAGFRNFAPIRKGQVMAQDNHGAVLAVED
                     GLVLLPLYQGKGSDGFFIAREVRPVWLVLSALLRRLRLDRLMRLLPGVRRSSGADVLV
                     ADTRIARFYPLEIFHLMGFRKVRRDGDYLVVARRKYDDRRPARIEFPAS"
BASE COUNT          628 a         1286 c         1283 g          611 t
ORIGIN      
        1 cgcgcacatg ggtcgcccgg gcgccgcggt ctcggatctc gagagctacc tcgagctgac
       61 ccccgacgcg cgcgacgccg aatcggttcg cgggcgggtc gcgctcttgc ggcagcggct
      121 gtccgagttc aactgagctc cgccggtcgg tcgcccccgt ttggcgccgg catttgaaac
      181 gcgcccggcg catgtagaat aatgggcgtt gcatgggccg gagtttccgc cgcccgagcg
      241 caaatgtcgc gccggttcgc ggatcctcac ctcggagcac cgctctcccc agggaaacga
      301 ccttccgatg accatcgagg caccgcccaa gagcccgaag gccaagccgc aacggctcga
      361 gcgcgcagtc atccgcttcg ccggcgactc cggtgacggc atgcagttga ccggcatgca
      421 gttcaccgcg gccaccgctg ccgcgggtaa cgacttggcc acgttgcccg actttcctgc
      481 cgagattcgc gcccccgcgg gcacgatggc cggcgtgtcg ggtttccaga tccagttcgc
      541 gtccgacacc gtcttcacgc cgggcgatcg cccggacgtg ctggtcgcga tgaacccggc
      601 ggcgttgcgg gccaacctgg ccgatctcgc cccgggcggg atcctgatcg tcaacgtcga
      661 ctcgttcaag gacaacgatc tgcgcaaggc cggctacgag cgcaacccgc tcgaggacgg
      721 ctcgctcgac ggctaccagg tgttcaagac cgagttgacg cggctgacgc gtgccgcgct
      781 gaaggaggtc gatctcccga cgcggtcggc cgatcggtgc aagaacttct tcgcgctggg
      841 gatgatgtac tacctgtacc accgtccgat gacggtgacg ctcgcctgga tcgagcggaa
      901 gtttgctggc aaggagttgt tgatcgaggc caatcggctc gcgctcaagg cgggttacgc
      961 gtactgcgag gcgaccgagc agtttgcgag ctcgtacgag gttcctgccg caccgctcga
     1021 gccgggcacg taccgcaaca tctcgggcaa ccaggcgctg gcgctcggct tcgtcgccgc
     1081 cgcgcgtcgc gcgggtctga ccctgttcca gggttcgtac ccgatcacgc cggcctccga
     1141 cctgctgcac gagctgtcga ccttcaagaa ccacggcgtg atcaccttcc aggccgagga
     1201 cgagatcgcc gcgatcggcg ccgcgatcgg cgcgtcgttc gcgggctcgc tcggcatcac
     1261 ctcgaccagc ggtcccggct tggcgctcaa gagcgagttt ctgtcgctgg cgatcatggc
     1321 cgagctaccg ctggtcgtcg tcgacgtgca acgcgcgggc ccgtcgaccg gaatgccgac
     1381 gaagaccgag caggcggacc tgctgatggc gctctacggc cggttcggcg agtcgccctg
     1441 tatcgtgctg gcgccgagca ccccggcgga ctgcttcaac gcggcctacg aggcgtgccg
     1501 gctgtcgttg ctgcacatga cgccggcgat cctgctgtcg gacggcttca tcgccaacgg
     1561 tgccgagccg tggaagctgc ccgagcccga tgagctgccg ccgattccgg tcgcctttca
     1621 ccggcaggtc gacggcttcc agccctaccg gcgggatccg cacacgctct cccggccgtg
     1681 ggccgtcccg ggcacggcgg gcctcgagca ccggatcggc gggctggaaa aggcggaagg
     1741 cagcggcaac gtctcctacg atccggcgaa tcacgaggcg atggtcaagc tgcgccaggc
     1801 caaggtcgac cgcgtggccg acgacattcc gccggccgag gtgaccggcg acgccgacgg
     1861 cgagttgctc gtcgtcggct ggggctcgac ctacggcgcg atcacgagcg cggtcagacg
     1921 tgccacgcaa gcggggctgc gtgtcgccca gctgcacctg cgtcacctga acccgctgcc
     1981 gtcgaacctc ggcgagattc tcgatcgctt cccgcgagtg ctggtagcgg agatcaacat
     2041 gggccagttg gcgttcgtgc tgaagggcag gttcgtcaaa cccgtgctca gtctgaccaa
     2101 gattcagggt acgccgttcg cggcctccga ggtagtggcg gagatcgaac ggctgctggg
     2161 gacgagagcg aatgaccact gagatcaagc tgtccaagaa ggacttcacc tcggaccagg
     2221 aagttcgctg gtgtccgggc tgcggcgact acgcgatcct gtctgcggcg cagcaggtct
     2281 ttcccgaatt gggcgtcccg cgcgagaagt tcgtcatcgt gtcgggcatc ggctgctcca
     2341 gccgattccc gtactacatg aagacctacg gcttccacac gattcacggg cgtgcgccga
     2401 gcgtcgcgac cggggtgaag ctggccaacc cggagctatc ggtgtggatc gtcaccggag
     2461 acggcgacgg gttgtcgatc ggcggcaatc acatcatcca cctgttccgg cgcaacgtcg
     2521 acgtcaacgt gatgctgttc gacaaccgta tctacgggct gaccaagggt cagtactcgc
     2581 cgacgtccga gacgggcaag cggacgaaat cgaccccgtt cggttcggtc gaccggccgt
     2641 tcaacccgtg cgcgttggcg ctcggcgccg gcgcgacgtt cgtcgcgaga agcgtcgaca
     2701 tctacgccga ccacctgcgg cagacgctga agacggcaca cgcgcatcgc ggaacctcgt
     2761 tcattcagat tttccagaac tgcaacatct tcaacgacgg cgcgttcgag cagatccgcg
     2821 aaaaggggct gcgcgaggag cacctgctgt tgctcgaaca cggcaagccg ctggtcttcg
     2881 gcaaggacct cgatcggggg atccgactgt cgggctgcaa ccggccggag atcgtcgagc
     2941 tgggaaacgg cgtcggcgag agcgacctga tggtgcacga cgaggccgcg ccgatcggct
     3001 acgccgcgat gctgagcgag atgcaaccgc cgagttggcc gatgccggtc ggcgtgatcc
     3061 ggcgtatcga cgcaccgtgc ttcgacgccg ccgtgcaccg gcagatcgcc gacgtcaccg
     3121 ctgatcgcgg caacggcgac ctgcgggcgg cgatccactc cggcaacacc tggaaggtca
     3181 gctagccggg aactcgatgc gggccggccg ccggtcgtcg tacttgcggc gggcgacgac
     3241 caggtagtcg ccgtcgcgtc ggaccttgcg gaatcccatc aggtggaaga tctcgagcgg
     3301 atagaaccgg gctatcctcg tgtcggcgac gagcacgtcg gcgccgctgg aacgcctgac
     3361 cccgggaagt agccgcatca gcctgtcgag tctcagcctg cgcagcagcg ccgacagcac
     3421 gagccacacc ggccgcacct cgcgggcgat gaagaatccg tcgctgccct tcccctggta
     3481 cagcggcaac aacaccaacc cgtcctcgac ggccagcacg gcgccgtggt tgtcctgggc
     3541 catcacctgg cccttgcgga tcggagcgaa gttgcggaat ccggctccca tccggaaccc
     3601 gtcggtcgcg cgcagggcat ggcggtggcg cacctcgatc acccgcggga tgccgcggct
     3661 ctgttcgcga aggaagcgcc ggtggcggtt gatgtcggga gtcgctcggg cgtcgatcgc
     3721 gccggcggcg acgagcgcga tccacagaat cgcctcggac cggtcgaccg aaccgggcgc
     3781 atcgtgctgg ccggcctcga ttccgaga
//
LOCUS       DASPGN010000329         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1697910, whole genome shotgun sequence.
ACCESSION   DASPGN010000329 DASPGN010000000
VERSION     DASPGN010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697910"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VD788_09535"
     CDS             <1..188
                     /locus_tag="VD788_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76549.1"
                     /translation="CNQPWEMPRLYAALHRNILDVFNEYGVQIMTPAYEGDPDQPKIV
                     PREQWHTPPAEPPPASR"
     gene            376..1515
                     /gene="asnA"
                     /locus_tag="VD788_09540"
     CDS             376..1515
                     /gene="asnA"
                     /locus_tag="VD788_09540"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HXV76550.1"
                     /translation="MTHTTDKQADLAGPGIGDYDQLERVLPADYRRLLDPKETQRALF
                     AAKRYIEDRLCAELNLIPVTVPLIVDAESGVNDYLDRDGSRTPIQFHISNDHGQNPID
                     AQVVQAATKWKRMALRQFGMLPGEGILTDMRAVRKDYFLDHDHSAYVDQWDWELAITE
                     EQRTLGYLTDVVKKLWKVIKGAERQVQQLFPALRSTSYPDLPDELTFMHAEDILARYP
                     DLPRKARETAILEEHPALFIYGIGWTLADGYPHEMRAADYDDWVTETTSVDGRPMHGL
                     NGDILVWNPVTRRRHELSSMGIRVDADTLRRQLELTGQLDFLDFPYHRAILDGAIPLS
                     IGGGIGQSRTYMTLLRKAHLGEVSVSVWPKKLKRICEARGIRVLD"
     gene            1661..2173
                     /locus_tag="VD788_09545"
     CDS             1661..2173
                     /locus_tag="VD788_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76551.1"
                     /translation="MDRVIEVGVLRRRRVRRVAVGAGCAAGLLVLVVGLPRLLRPSLP
                     SGTLRIATVERGEVEALLQPSVLGYARGVGVGDRGLDPEQIEVGDASQHVPRSYGATD
                     VGDDPGQHAVVLRARLGQLEPAEAALRQTEVEGRTVPDASTSALRRLSDLAGRREAKR
                     LDAELNRYRL"
     gene            2191..2823
                     /locus_tag="VD788_09550"
     CDS             2191..2823
                     /locus_tag="VD788_09550"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXV76552.1"
                     /translation="MKDEPELGEFKFRVNNRWIDGTLNRAENPAFYGAGAGDTGRTRS
                     HVHQIDEPPVLLGEDRGANPVEYLLTGLSGCVTTTFVAYAAAQGVKTDELRTELEGVL
                     DVRGFLGVSGRVRPGYRQIRVRFHVKSDAPREKVEELIKLAERRSPVSDSVRKGVRVD
                     FSLVMRRALDGLEVIGTGGAPGVLGWVARAAGEPPTERIGGSKNGRETDT"
     gene            2801..3133
                     /locus_tag="VD788_09555"
     CDS             2801..3133
                     /locus_tag="VD788_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76553.1"
                     /translation="MGAKRTRDLVLVLSLVGLVLACGQQGEQAEQAPDAMSEAASEVE
                     DTMSETAEDVRAETERLIAEKQDEIAQLTAQLEEASVESAQALREQLEAAQQELADLE
                     SRLSELTN"
BASE COUNT          561 a         1064 c         1110 g          458 t
ORIGIN      
        1 actgcaacca gccctgggag atgccacgat tgtacgccgc gcttcaccgc aacatcctcg
       61 acgtgttcaa cgaatacggc gtacagatca tgacgccggc gtacgaaggc gaccccgacc
      121 aaccgaagat cgtgccgcgc gagcagtggc acacccctcc cgccgagcca ccgccggcgt
      181 cgcgctagcg ctcaggacga tcaccggcgg cgccgatcac gggaccagcg gccgccagcg
      241 gaataggccg gacggcccgg gcgaccccgc cgccattaga ggtcccttga cgcgagaggc
      301 ggtcgcgcga gccggtgcta tgtttcgttt ccacgggagg gtcggctcga cccgggatca
      361 aggaggtaag caccgatgac gcacaccacc gacaagcagg ccgatctcgc gggtccgggc
      421 atcggcgact acgaccagct cgaacgggtg ctgcccgccg actaccggag gttgctcgac
      481 ccgaaagaga cccaacgcgc gctgttcgcg gccaagcgct acatcgaaga ccgactgtgt
      541 gccgagctga acctgatccc ggtgacggtc ccgctgatcg tcgacgcgga aagcggggtc
      601 aacgactacc tcgaccgcga cggatcccgc acgccgatcc agttccacat ctcgaacgac
      661 cacggtcaga acccgatcga tgcgcaggtc gttcaggcgg cgaccaagtg gaagcggatg
      721 gcgttgcggc agttcggcat gctaccgggc gagggcatcc tcaccgacat gcgcgcggtg
      781 cgcaaggact acttcctcga ccacgaccac agcgcctacg tcgaccagtg ggactgggag
      841 ctggcgatca cggaggagca acgcaccctg ggctacctga ccgacgtcgt gaagaagctg
      901 tggaaggtga tcaagggggc cgagcgacag gtccagcagc tgttcccggc gctccgctcg
      961 acgagctacc cggaccttcc cgacgagctg accttcatgc acgccgagga catcctggct
     1021 cgctatcccg atctgccgcg caaggcgcgc gagaccgcga tcctggagga gcacccggcg
     1081 ctgttcatct acggcatcgg ctggacgctg gccgacggct acccgcacga aatgcgcgcg
     1141 gcggactacg acgactgggt gacggagacg acctcggtcg atggacgtcc gatgcacggt
     1201 ctgaacggcg acattctcgt ctggaacccg gtcacgcgcc gacgccacga gttgagctcg
     1261 atgggcatcc gggtcgacgc cgacacgctt cgccgacagc tcgagctgac cggtcagctc
     1321 gacttcctcg acttccccta ccaccgtgcg atcctcgacg gggcgatccc gctcagcatc
     1381 ggcggcggga tcggacagtc gcgaacctac atgacgctgt tgcgcaaggc ccatctgggc
     1441 gaggtcagcg tcagcgtttg gccgaagaag ctcaagcgga tctgcgaggc gcgcgggatc
     1501 cgggtcctcg actgagctat cggtaggcgg gaccccgcgc cggaccgtcc agcgccggac
     1561 ggaggcgttc gaaaacgaac gatcctcgac gccgggccga gcggattccg gcgaaaacca
     1621 ccggctccca ccctgatccg cggcttgcga gagcgcaacc gtggaccgcg tgatcgaagt
     1681 cggtgtgctg aggaggcggc gggtgcgacg ggtcgccgtc ggcgccggct gcgccgccgg
     1741 tctgttggtt ctcgtcgtcg gtctaccccg gctgcttcga ccctcgttgc cgagcggtac
     1801 gttgcgcatc gccaccgtcg agcgaggcga ggtcgaggcg ctgctgcaac cttctgtcct
     1861 cggctacgcg cgaggcgtag gtgtcggcga ccgtggcctc gatccggaac agatcgaggt
     1921 cggcgatgcg agccagcacg tcccccggtc gtacggtgct accgacgtcg gcgacgaccc
     1981 aggtcagcac gccgtcgtcc tccgcgcgcg cctcggccag ctcgagccgg ctgaggcggc
     2041 gctgcgacag accgaggtcg agggcaggac ggttcccgac gcctcgactt ccgccttgcg
     2101 caggctcagc gatctcgcgg gccggcggga agccaaacgg ctcgacgcgg agctgaaccg
     2161 ttaccggctg taggggagac gatcgagcgg ttgaaggacg agcccgagct gggggagttc
     2221 aagttccgcg tcaacaaccg gtggatcgac gggacgctga atcgcgcgga aaaccccgcc
     2281 ttctacggcg cgggagcggg ggacaccggc cgtacccggt cgcacgtcca tcagatcgac
     2341 gagccgccgg tgctgctcgg ggaggaccgt ggcgccaacc cggtcgagta cctgctgacc
     2401 ggcctctccg gctgcgtgac gacgaccttc gtcgcctacg ccgccgcaca gggagtaaag
     2461 accgacgagc tgcggaccga gctcgagggg gttctcgacg tgcgtggctt tctcggcgta
     2521 tcggggcggg ttcgccccgg ctatcgccag attcgcgtgc gcttccacgt caagtccgac
     2581 gccccgcgcg agaaggtcga ggaactgatc aagctcgccg agcgtcgctc gccggtgtcg
     2641 gacagcgtgc gcaagggcgt gcgggtcgac ttctcgctgg tgatgcggcg ggcgctcgat
     2701 ggcctcgagg tcatcggcac cggaggcgcg cccggtgtgc taggctgggt tgcccgcgcg
     2761 gccggcgagc cgccgaccga acgcataggg gggtcgaaga atgggcgcga aacggacacg
     2821 tgatctggtg ttggttctct cgctggtcgg actcgtcctg gcctgtggcc agcagggcga
     2881 acaggccgag caagcgccgg acgcgatgag tgaggcggcg tccgaggtcg aggacaccat
     2941 gtccgaaacg gccgaggacg tccgagccga aaccgagcgg ctgatcgcgg agaagcagga
     3001 cgagatcgcg cagttgaccg cgcagctcga ggaagcctcc gtcgagagcg cgcaggcgct
     3061 gcgcgaacag ctcgaagcgg cgcagcagga gctggcggac ctggaaagca ggctctccga
     3121 gctcacgaac tgagccgcgg tcggcgggcg tccgcccggc cgaccccctc gtgagttgct
     3181 gaatttgcgt ctt
//
LOCUS       DASPGN010000330         3402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_760859, whole genome shotgun sequence.
ACCESSION   DASPGN010000330 DASPGN010000000
VERSION     DASPGN010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760859"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..764)
                     /locus_tag="VD788_09560"
     CDS             complement(54..764)
                     /locus_tag="VD788_09560"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV76554.1"
                     /translation="MKASKQALGIGFVSMLMVAGCAPAGDTAPPVEARSELPPEPVVT
                     YRGGDPERRVEHGELIVDVDEARRALHAQHHPHGVLETRRIPGEFTPPAMGEIADFAL
                     AKDDLIVINPGPGEYVHVMEGQRHGYRNLTIGITYTAPGGAPPMHTHFGEEAHVLTSG
                     QTIRYALGDEVFELQGPYVVNIPPMVPHSFQNLDDQVAELVVIFPTNVWEYDVLDYFP
                     FKDLPVGSTQPARTPSGR"
     gene            871..3075
                     /locus_tag="VD788_09565"
     CDS             871..3075
                     /locus_tag="VD788_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV76555.1"
                     /translation="MRVRTFLILLAFLAHAAGTEATDEAARNDAFWEAARAGDLETLK
                     RLHAEGVAIDTPTPYGATALSYACDKGQIDVVRYLVANGADVNVKDTFYEFDPLGWAV
                     FNEHDEIAVVLLENGAEDANSALVTGAGRGSEALVKAALASGRITAADVAEALERAKA
                     LDEPQQIVALLEAAEVKPSERKEIELLPETLAGYAGKFRNDDVEMTIEVRVEDGALVA
                     QATGQRPLALRPIAENVFETVGVPIEVTFEGRGGTIDRLVVDQGGQQTSFARIVETEG
                     EGAAAVAPPSDAVPRAPTTATPGIVRKAQQNWPSFRGPRASGVADGQGAPVEWNVSTG
                     DNVRWKTPIPGLANSSPIVWGDRVFVTTAISGGGDETFRTGLYGDVDSVDDESEHEFR
                     LYALDRASGAIVWERTVAQAVPGAKRHLKSTQANSTPATDGKRVVALFGTIGLLVAFD
                     LDGKELWRTDLGVLDAGWFYDRSYQWGHASSPVIHDGLVIVQADVYADSFIAAYRLED
                     GKQAWKAPREDVPSWGSPTVYDSGERAELITNGKTIKGYDPRTGKELWSLAPNSEVTV
                     ATPIVAHDLFYVTAGYPPVQPIYAIRPGGEGDLTLAGDAEESETIAWSKSRGGTYMPT
                     PIVYGGQLYTCANDGRLTAYDAGTGTPVYRARIGGGAGSFTASPIAADGRLYFTAETG
                     QIYVVKAGPAYQELAANEMDEVCMSTPAISDGLLVVRTLRHVYGLGEPPDPE"
     gene            3196..>3402
                     /locus_tag="VD788_09570"
     CDS             3196..>3402
                     /locus_tag="VD788_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76556.1"
                     /translation="MQQRVVRWLAAVVCTCCALSLPHSQVSIPRQTYLAFDGADFVRI
                     EDSPALDLAGGEFTFSAWILPTGWG"
BASE COUNT          548 a         1129 c         1204 g          521 t
ORIGIN      
        1 cagttcgcga tcggtgtgca gaccgtcgcc tcagcggggc gtgtgccagc ccatcaccga
       61 ccgctcgggg tgcgggcagg ttgtgtagac ccgaccggca ggtctttgaa cgggaagtag
      121 tcgagcacgt cgtactccca gacgttggtc gggaagatca cgaccagctc cgcgacctga
      181 tcgtcgaggt tctggaacga gtgcggcacc atcggcggga tgttgacgac gtagggaccc
      241 tggagctcga acacctcgtc gcccagcgcg tagcggatcg tctgacccga ggtcagcacg
      301 tgcgcctcct cgccgaagtg cgtgtgcatc ggcggcgcgc ctcccggcgc ggtgtaggtg
      361 atgccgatcg tcagattgcg atagccgtgg cgctgaccct ccatcacgtg cacgtactcg
      421 ccggggccgg ggttgatcac gatcaggtcg tctttggcca gcgcgaagtc ggcgatttcg
      481 cccatcgccg gcggcgtgaa ctcccccggg atgcgacgcg tctcgagcac gccgtgcggg
      541 tgatgctgtg cgtgcagcgc ccggcgcgcc tcgtcgacgt cgacgatgag ctcgccgtgc
      601 tcgacgcgcc gctccggatc gcctccccgg taagtgacga ccggctcggg cggtagctcg
      661 gagcgggcct cgaccggcgg ggccgtatcg ccggcaggcg cgcagcctgc gaccatgagc
      721 atcgagacga atccgatacc gagtgcctgc ttgctagctt tcatcgacgg ttctcctcgg
      781 cggtgcacgc gatgggctcc ggtcgggtac actccaccgg ccgggcggac cgagcatacg
      841 ccacggcacg gtcctcgggg aggctccacg atgcgcgtac gtactttctt gatcctgttg
      901 gcgtttctcg ctcacgccgc gggcacggag gccaccgacg aggcggcgcg caacgacgcg
      961 ttctgggagg cggcgcgcgc cggcgatctc gaaacgctga agcggctgca tgcggaggga
     1021 gtcgcgatcg acacgccgac accctacggt gcgaccgcac tgtcctacgc ctgtgacaag
     1081 gggcagatcg acgtcgtgcg ctacctcgtc gcgaacggcg ccgacgtcaa cgtcaaggac
     1141 accttctacg agttcgaccc gctcggctgg gcggtgttca acgagcacga cgagatcgcc
     1201 gtggtgttgc tcgagaacgg ggcggaggat gcgaacagcg cgctggtgac gggtgcgggc
     1261 cgcggcagcg aggcgttggt caaggcggcg ctcgccagcg gacggatcac cgccgccgac
     1321 gtcgccgagg cgctcgagcg cgcgaaggcg ctcgacgagc cgcagcagat cgtcgcgttg
     1381 ctcgaagcgg ccgaggtcaa accgtccgag cgcaaggaga tcgagctttt gcccgagacg
     1441 ctcgccggct acgccggcaa gttcaggaac gacgacgtcg agatgacgat cgaggtgcgc
     1501 gtcgaggacg gggcgctggt ggcgcaggcc accgggcagc gaccgctcgc gctgcgtccc
     1561 atcgccgaga acgtgttcga gacggtcggg gtcccgatcg aggtaacgtt cgagggtcgc
     1621 ggcggaacga tcgaccgtct ggtcgtcgac cagggcggtc agcagacgag cttcgccagg
     1681 atcgtcgaga ccgaggggga gggcgcggcg gccgtcgccc cgccgtccga tgcggtgcct
     1741 cgagcgccga cgaccgcgac gccgggaatc gtgcgcaagg cccagcagaa ctggccgtcg
     1801 ttccgcggtc cgcgagcgtc gggtgtcgcc gatggtcagg gagcgccggt cgagtggaac
     1861 gtgtcgacgg gcgacaacgt ccgctggaag acgccgatcc ccggtctcgc caactcgagc
     1921 ccgatcgtct ggggcgaccg cgtcttcgtc acgaccgcga tcagcggcgg gggagacgag
     1981 acgtttcgaa ccggactgta cggcgacgtc gattccgtcg acgacgagag cgagcacgag
     2041 ttccgtctct acgccctcga ccgtgcgagc ggcgcgatcg tctgggagcg gaccgtcgcc
     2101 caggcggtgc cgggcgccaa acgccacctg aaatcgaccc aggccaactc gacgccggcc
     2161 accgacggaa aacgggtcgt cgcgctgttc ggcaccatcg gtctcttggt ggcgttcgac
     2221 ctcgacggca aggagctgtg gcgcaccgat ctcggcgtgc tcgatgccgg ctggttctac
     2281 gaccgcagct accagtgggg gcacgcgagc tcgccggtga tccacgacgg cctggtgatc
     2341 gtgcaggccg acgtctatgc ggactcgttc atcgcggcct atcggctcga agacggcaag
     2401 caggcctgga aggcaccgcg ggaggacgtc ccgtcgtggg gctccccgac ggtctacgac
     2461 tcgggcgagc gcgccgagtt gatcaccaac ggcaagacga tcaagggcta cgacccgcgg
     2521 acgggcaagg agctgtggag cctcgcgccc aactcggagg tgactgtcgc gacgccgatc
     2581 gtggcgcacg acctgttcta cgtcaccgcg ggctatccgc cggtgcagcc gatctacgcg
     2641 atccgtccgg gaggcgaggg cgacctgacg ctcgccggcg acgccgagga gagcgagacc
     2701 atcgcctgga gcaagtcacg cggcggtacc tacatgccga cgccgatcgt ctacggcggt
     2761 cagctctaca cctgtgccaa cgacggccgg ttgaccgcct acgacgccgg aaccgggacg
     2821 ccggtctacc gcgcgcgcat cggcgggggc gccggctcgt tcacggcctc gccgatcgcc
     2881 gccgacggcc gcttgtactt caccgcagag accggtcaga tctacgtggt caaggccggg
     2941 ccggcctacc aggaactggc ggcgaacgag atggacgagg tgtgcatgag cacgccggcc
     3001 atttcggacg ggttgctggt cgtgcggacc ctgcgccacg tctacggcct gggtgaaccc
     3061 cccgacccag agtgattccc ccgatccggt agcaaggtcg gcggaaacgt cgtagatctc
     3121 cccgagggat tcgccagggg tcccgttttt cgctagactg aggccaggct tccggggatc
     3181 ggggacacac tcgacatgca gcagcgcgtc gtacgttggc tcgcggcggt ggtctgtacc
     3241 tgctgcgcac tctccctgcc ccattcccag gtctcgatac cgcgacaaac ctacctcgct
     3301 ttcgacggcg cggatttcgt ccggatcgag gacagcccgg cgctcgacct cgccggcggt
     3361 gagttcacct tcagcgcctg gatcctgccg accggctggg gc
//
LOCUS       DASPGN010000331         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1346123, whole genome shotgun sequence.
ACCESSION   DASPGN010000331 DASPGN010000000
VERSION     DASPGN010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346123"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1461)
                     /locus_tag="VD788_09575"
     CDS             complement(<1..1461)
                     /locus_tag="VD788_09575"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV76557.1"
                     /translation="MSERTGESVIGRSECTRCGAHFVQEDDLLGACPRCLIGVGLADE
                     VDDAEGDAPRAGPADAIGERFGSYRLVDLLGEGGMGVVYLADQHQPIRRKVAIKLIKL
                     GMDTREIVARFESERRTLARMDHPNIAKVHEAGVSEDGRPFFVMEYVPGTTITRYCDD
                     RTLDVASRVRLFIDVCRAIQHAHQKGVIHRDIKPTNVLVTPVDGAPVVKVIDFGVAKA
                     IDPLDTGQSWFTRRGVMIGTPEYMSPEQAAGSGTDVDTRTDVYALGLILYELLTGNAA
                     IDRERLRTMSLEQIRASIRDEELPRPSARYHSTATGRAAIARLRRTTPVGLGRELRGD
                     LDWIVMTALAKDRERRYASPGELADDLERHLDNRPVLAGPPTAAYRLRKFVLRHRVAA
                     TFGGSMLVLLIAFTIGMTVLAGRIARERDRANLEARAASQVSQFMIDLFEVSDPGEAL
                     GETIPAREILERGARRIEQELGDQPALQARLMATIGT"
     gene            complement(1503..2231)
                     /locus_tag="VD788_09580"
     CDS             complement(1503..2231)
                     /locus_tag="VD788_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76558.1"
                     /translation="MSRTRRRGATGRFVTTEWSLVVASAEGGAAAGEALAELCRIYWP
                     PVYAHLRRRGADPERALDLTQGFFTKLLEKNYIADARRERGRFRTFLLTSLHHYTANE
                     WDRDHALKRGGNRSPIPIDAADAERGYLFEPSHDETPERVFDRRWARTLLRTVLDRLR
                     GDSKEREASGFDRIAPFVVGGQESGYRAIAGELGTTEAAVRVRVHRLRRRFRALVREE
                     VARTVVDERQIELEMRHLFRVLAG"
     gene            complement(2316..>3011)
                     /locus_tag="VD788_09585"
     CDS             complement(2316..>3011)
                     /locus_tag="VD788_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HXV76559.1"
                     /translation="DKGVLTFPWGNGLRAEDLESFWEAQTFPDGGPFYDWVHGETGAR
                     VFKVQHPEFELWSQGIHAKSGVACADCHMPYERVGAMKLSSHDVRSPLERIGTACVTC
                     HGVDETDLRERVAAVQATTVALTERAGAAMTEMLDAILEAKAAGASDDDLAEVFSLQR
                     KAMWRLDYISSENSNGFHADQEAARLLGESIDYSRRAQAAALRWRAPAAPATDALPVE
                     PIRGVTPDDGPAG"
BASE COUNT          440 a         1088 c          988 g          495 t
ORIGIN      
        1 ggtgccgatc gtcgccatca gtcgggcctg cagcgccggt tggtcgccca gctcctgctc
       61 gatcctgcgc gccccgcgct cgaggatctc gcgtgccggg atcgtctccc ccagcgcctc
      121 gccgggatcc gacacttcga acaggtcgat catgaactgc gacacctgac tcgccgcgcg
      181 cgcctcgagg ttggcgcgat cgcgttcgcg cgcgatgcgg ccggccagca cggtcatccc
      241 gatcgtgaat gcgatcagca gcaccagcat cgatccgccg aacgtcgcgg cgacgcgatg
      301 ccgcaggacg aacttccgca ggcgataggc cgcggtcggc ggcccggcga gcaccggccg
      361 gttgtcgagg tgacgctcga ggtcgtccgc cagctcgccc ggcgaggcgt agcgccgctc
      421 gcggtccttg gccagcgcgg tcatcacgat ccagtcgaga tcgccacgca gctcccggcc
      481 gagaccgacc ggggtggtcc gccgcaagcg cgcgatcgcc gcccggccgg tggcagtcga
      541 gtggtaccgc gcgctcggcc gcggcagctc ctcgtcgcgt atcgacgcgc ggatctgctc
      601 caggctcatc gtgcgcagtc gctcgcgatc gatcgccgcg ttgccggtca gcagctcgta
      661 gaggatcagc ccgagcgcgt agacgtcggt ccgggtgtcg acgtccgtgc cggagccggc
      721 cgcctgctcg ggagacatgt actcgggcgt cccgatcatc accccgcgac gggtgaacca
      781 gctctgcccg gtgtcgagcg ggtcgatcgc cttggccacg ccgaagtcga tgaccttgac
      841 caccggtgcc ccgtcgaccg gggtgaccag tacgttcgtc ggcttgatgt cgcgatggat
      901 cacgcctttc tgatgcgcgt gctggatcgc gcggcagacg tcgatgaaca accgcacacg
      961 cgatgcgacg tcgagtgtcc ggtcgtcgca gtaccgcgtg atcgtcgtgc cggggacata
     1021 ctccatcacg aagaacggac gaccgtcttc gctcacgccg gcctcgtgca ctttggcgat
     1081 gttggggtgg tccatccgtg ccagcgtccg gcgctccgac tcgaaccgag cgacgatctc
     1141 gcgcgtgtcc attcccagtt tgatcagctt gatcgccacc ttccggcgga tcggctggtg
     1201 ctggtcggcg agatagacga cacccatgcc gccctcgccg agcagatcga ccaagcggta
     1261 cgatccgaac cgctcgccga tcgcgtccgc ggggccggct cgcggcgcat cgccctcggc
     1321 gtcgtcgacc tcgtcggcca gcccgacccc gatcaggcag cgcggacagg cccccagcag
     1381 gtcgtcctct tgcacgaagt gcgcgccgca gcgggtgcac tcggatcgcc cgatcacgct
     1441 ctcccccgtt cgttcactca tcgttgaagc acggcctggt gctcgttacg cgtcgcggtg
     1501 gatcagccgg ccaatacccg aaacagatga cgcatctcga gctcgatctg gcgttcgtcg
     1561 acgacggtcc gcgccacctc ttcgcggacc aacgcgcgaa accgccggcg caatcggtgt
     1621 acccggaccc gcacggccgc ctcggtcgtc cctagctcgc ccgcgatggc gcggtagccg
     1681 ctctcctgtc cgccgaccac gaacggggcg atccgatcga atccactcgc ttcgcgttcc
     1741 ttgctgtcgc cccgcaggcg atcgagaacc gtgcgaagca gcgtgcgggc ccaacgtcgg
     1801 tcgaagaccc gttccggcgt ctcgtcgtga ctcggctcga acaggtagcc gcgctcggcg
     1861 tccgcggcat cgatcggaat cggagaccgg ttcccgcctc gcttcagcgc gtggtcgcga
     1921 tcccattcgt tggcggtgta gtggtgcaac gaggtgagaa ggaacgtgcg gaagcggccc
     1981 cgttcgcgac gcgcgtccgc gatgtagttc ttctcgagca gcttggtgaa gaagccctgc
     2041 gtcaaatcga gcgcacgctc gggatcggcg ccgcgacgcc tcaggtgggc gtagaccggc
     2101 ggccagtaga tgcggcacag ctcggccaac gcctcgccgg ccgcagcgcc gccttccgcg
     2161 gacgcgacga ccagcgacca ttcggtcgtg acgaagcgcc ctgtcgcacc tctccgccgg
     2221 gttcgggaca cgagacctca cgagctgaaa cgaagtcgaa tgctgtcaag agtttatcac
     2281 aagggtcgcc gcccgccgag cggaacggcc cggcgtcaac cagccgggcc gtcgtccggt
     2341 gtgacgcctc ggatcggctc gaccggtagc gcgtccgtcg ccggtgcggc gggggcgcgc
     2401 cagcgcagcg cggcggcttg cgcccgccgg ctgtagtcga tcgactcccc gagcagtcgc
     2461 gcggcttcct ggtcggcgtg gaagccgttc gaattctcgc tgctgatgta gtcgagacgc
     2521 cacatcgcct tgcgctgaag cgagaatact tcggccaggt cgtcgtcgct cgccccggcc
     2581 gccttggcct cgaggatcgc atcgagcatc tcggtcatcg cggcccccgc ccgctcggtg
     2641 agcgccacgg tcgtcgcttg caccgccgcg acacgctcgc gcaggtcggt ctcgtccacg
     2701 ccgtgacagg ttacgcacgc ggtgccgatc ctctccagcg ggctccgcac gtcgtggctg
     2761 ctcagcttca tcgctccgac gcgctcgtag ggcatgtggc agtcggcgca agcgaccccg
     2821 ctcttggcat ggatgccctg gctccacagc tcgaactccg ggtgctgcac cttgaagact
     2881 cgggcgcccg tctctccgtg cacccagtcg tagaacgggc ccccgtccgg aaacgtctgc
     2941 gcctcccaga agctctcgag gtcctccgcg cgcagcccgt tgccccacgg aaaggtgagg
     3001 acgcccttgt c
//
LOCUS       DASPGN010000332         3650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_175837, whole genome shotgun sequence.
ACCESSION   DASPGN010000332 DASPGN010000000
VERSION     DASPGN010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175837"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1945)
                     /locus_tag="VD788_09590"
     CDS             complement(<1..1945)
                     /locus_tag="VD788_09590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXV76560.1"
                     /translation="MTHERERRERKAIPRIGRQWVRRAVCGLFLLLSRHVVPAAEVAL
                     FEFGSANRYLANSSDPGVGLAWTQPGFDDSIWQQGTYGIGYDDAGSALALLQTQVPSG
                     VMSVYTRTTFQITETELAEVSQLTLGVDYDDGFVAWLNGVEIWKASLPSNPAWDTPAS
                     ATESSNGTQPSYRRFDVTRNIPQLQVGTNVLAVGVWNTHTGSSDLVLAPAMWKNADAA
                     LIRGPYLQSGSPTSIVVRWRTNGATPSRVLYGPSAGSLAQVVEDPTAVEDHEITLTGL
                     DPDTTYYYAVGTDSEILAGDDAQHFFVTAPLSGTAKPTRIWVLGDSGTGDANATAVRD
                     AYYAYTGERHTDLWLMLGDNAYDDGTDLEFQTKLFDIYPEMLRKSVLWPTLGNHDGVT
                     ADSATQTGPYYDIFTLPKGGEAGGVASGTEAYYSFDYGNVHFIVLDSFETSRTLLSPM
                     RIWLEQDLAATTQDWIIAFWHHPPYSKGSHDSDTEYQQVDMRQNFVPLLDEYGVDLTL
                     TGHSHSYERSYPIVGHYGTSDTFDESMKTDPGDGREDGGGPYQKPALGPNPHSGIVHT
                     VAGNGGRLSSGTFDHPAMFLSLMELGSLIIDVNQTRLDLRLLDRNGAILDYLTVFKGP
                     IVVAPVADFDATPPSGNVPLLVDF"
     gene            complement(1968..2816)
                     /locus_tag="VD788_09595"
     CDS             complement(1968..2816)
                     /locus_tag="VD788_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76561.1"
                     /translation="MRTIIVSALLLSCFTATAVSSHPAIDDQIKALTARIADDSADAS
                     LYLRRGELHRIHGEWDKAERDYRAALQLDGDLVIVEFCLGRLKLESGHAEEAKTALDS
                     YLARRPEDATALATRAQASSALGNHLAAAEDYTIAIEHSRSEAPKPEYFLARARELAA
                     AGTDHLATAIAGLDEGLEQLGHPVTLQLYAIDLELERKNFDEALRRIDRVSSESVRQE
                     PWLVRKAAILEAAGRVAEARQVYQQTLASLDALPATRKNNRAVVRLEDEARQGLKRLS
                     PEEAGP"
     gene            complement(2910..>3650)
                     /locus_tag="VD788_09600"
     CDS             complement(2910..>3650)
                     /locus_tag="VD788_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76562.1"
                     /translation="CVDGDSDLYFTGCDAYTTRSGPDCDDSIASCTNDCTTDTDGDTI
                     PDCADDCADADDDGYGVGAGCAGADCSDTDVDNWTSCATCVDGDSDLYFTGCDAYTTR
                     SGPDCDDSIASCTNDCTTDGDGDTIPDCADDCADADDDGYGVGTGCAGADCSDTDVDN
                     WTSCATCVDGDSDLYFTGCDAYTTRSGPDCDDTSGSTFPGAAPLDDSDACMNDDDGDD
                     WGDKSPPGGVVPGSDCDDTNLAVAPGTC"
BASE COUNT          593 a         1154 c         1230 g          673 t
ORIGIN      
        1 cgaagtcgac gagcagtggc acgttaccgg acggcggggt cgcatcgaag tcggccacgg
       61 gcgcgacgac gatcggcccc ttgaaaacgg tcaagtagtc gagaatcgcg ccgttgcgat
      121 cgagcagccg cagatcgagg cgagtctggt tgacgtcgat gatcagcgag ccgagttcca
      181 tcaacgacag gaacatcgcc ggatggtcga acgtcccgct cgagagtctg ccgccgttgc
      241 cggcgacggt gtggacgatt cccgaatgag ggttcggtcc gagcgcaggc ttctgatagg
      301 gcccgccacc atcctcccgg ccgtcgcctg gatcggtctt catcgactcg tcgaaggtgt
      361 ccgacgtgcc gtaatgaccg acgatcgggt aagaccgctc gtagctgtgg ctgtgaccgg
      421 tcagtgtcag atcgacgccg tactcgtcga gcagtggaac gaagttctgc cgcatgtcga
      481 cctgctggta ctcggtgtcc gagtcgtgac tgcccttgct gtacggcgga tggtgccaga
      541 acgcgatgat ccagtcctga gtcgtggcgg cgaggtcctg ctcgagccag atacgcatcg
      601 gactgagcag cgttcggctc gtctcgaacg agtccagcac gatgaagtgg acgttgccgt
      661 agtcgaacga gtagtacgct tcggttccgg acgcgacccc acccgcctcg ccgcccttgg
      721 ggagcgtgaa aatatcgtag tacgggccgg tctgggtcgc cgaatcggcg gtgactccgt
      781 cgtggttccc caacgtcggc cacaacaccg acttccgcag catctccggg tagatgtcga
      841 acagcttggt ctggaactcg agatcggtac cgtcgtcata ggcgttgtcg ccgagcatca
      901 accacaggtc ggtgtggcgt tcgccggtgt acgcgtaata cgcgtcgcgc accgccgtcg
      961 cgttggcatc gcccgtaccg gaatcgccga gcacccagat tcgcgtcggt ttggccgtgc
     1021 cgctgagcgg ggcggtgacg aagaaatgct gtgcgtcgtc gccggccagg atctcgctgt
     1081 cggtccccac ggcgtagtaa taggtggtgt cgggatcgag tccagtcagc gtgatctcgt
     1141 ggtcttcgac cgccgtaggg tcctcgacga cctgcgccaa gcttcccgcg gacgggccat
     1201 agagcactcg gctcggcgtc gccccgttcg tacgccagcg cacgacgatg ctggtcgggc
     1261 tgccggactg caaatacggg ccgcggatga gggccgcatc ggcgttcttc cacatcgccg
     1321 gagcgagcac caggtcgctc gagcccgtgt gcgtgttcca cacgccgacc gccagcacgt
     1381 tcgtgccgac ttgcagttgc gggatgttgc gggtcacgtc gaagcgccgg tagctcggtt
     1441 gcgtaccgtt gctcgattcg gtggccgagg ccggcgtatc ccacgcgggg ttcgagggca
     1501 gactcgcctt ccagatctcg actccgttca gccacgcgac gaagccgtcg tcgtagtcga
     1561 cgcccaacgt gagttgcgaa acctcggcga gctcggtctc cgtgatttgg aaggtggtgc
     1621 gcgtatagac cgacatcacg cccgacggca cttgagtctg aagcagcgct agggcgctgc
     1681 ccgcgtcgtc ataaccgatg ccgtacgtgc cctgttgcca gatcgagtcg tcgaagcccg
     1741 gctgggtcca tgccagcccg acccccgggt cggacgagtt cgccaggtac ctgttcgccg
     1801 acccgaactc gaacagcgcg acctcggccg cgggaaccac atgcctggac agcagcagga
     1861 agagcccgca taccgcgcgg cggacccact gccggccgat gcgtgggatg gcttttcgct
     1921 cgcgtcgctc ccgttcgtgc gtcaaggcgg ggatgaactc ggccgttcta cggcccggct
     1981 tcttcgggag acaggcgctt cagcccctgt cgcgcctcgt cctccagacg taccaccgca
     2041 cggttgttct ttcgggttgc ggggagtgcg tccagtgaag cgagcgtctg ctgatacact
     2101 tgtcgagcct ctgccacgcg accggccgcc tcgaggatgg ccgccttgcg aaccagccac
     2161 ggttcctggc gcaccgactc gctggagacc cgatcgatcc gccggagcgc ttcatcgaag
     2221 ttcttgcgct cgagctcgag gtcgatggca taaagctgca gcgtcaccgg gtggccgagt
     2281 tgctcgaggc cttcgtcgag gccggcgatc gccgtcgcca ggtgatcggt tccggccgcg
     2341 gcgagctcgc gggcacgtgc caggaagtac tccggtttcg gggcctcgct tcggctgtgc
     2401 tcgatcgcga tcgtgtagtc ctcggccgcc gccaagtggt tgccgagagc actcgacgcc
     2461 tgggcgcgtg tcgccagcgc ggtcgcgtcc tccggccggc gggccaggta cgagtcgagg
     2521 gccgtcttcg cttcctcggc gtggcccgat tcgagcttga gccgcccgag acagaactcg
     2581 acgatgacca agtcaccgtc gagttgcagc gcggcacgat agtctcgttc ggctttgtcc
     2641 cactcgccgt ggattcggtg gagctcaccg cgccgcaggt acagactcgc gtcagccgag
     2701 tcgtccgcga tgcgagcggt cagcgccttg atctgatcgt cgatcgcggg gtggctcgac
     2761 accgcggtgg cggtgaaaca agacaagagg agcgcggaaa cgatgatggt acgcaagatt
     2821 gtaattcctc tagaagagaa gttgaccgcc cgccgtatat gtacgcagat ttcacgttcc
     2881 gtccacaccg accgtgaacg gtatgaggct caacaggttc cgggagccac cgcgaggttg
     2941 gtgtcgtcgc aatccgatcc gggtacaacg ccgcccgggg gtgacttgtc gccccaatcg
     3001 tcgccgtcgt cgtcgttcat gcaggcgtcg gaatcgtcta ggggcgccgc tccggggaag
     3061 gtactgccgg aggtgtcatc gcagtcggga cccgagcgag tcgtgtaggc atcgcacccg
     3121 gtgaagtaga ggtcggagtc gccgtcgacg caggtcgcgc aggaagtcca gttgtcgacg
     3181 tcggtgtcgc tgcaatcggc tcccgcgcaa ccggtgccga caccgtagcc gtcgtcgtcc
     3241 gcatcggcgc agtcgtcggc acagtcgggg atcgtgtcgc cgtcgccgtc cgtggtgcag
     3301 tcgttcgtgc agctcgcgat cgaatcgtcg cagtcgggac ccgagcgagt cgtgtaggca
     3361 tcgcacccgg tgaagtagag gtcggagtcg ccgtcgacgc aggtcgcgca ggaagtccag
     3421 ttgtcgacgt cggtgtcgct gcaatcggct cccgcgcagc ccgcgccgac accgtaaccg
     3481 tcgtcgtccg cgtcggcgca gtcgtcggca caatcgggga tcgtgtcgcc gtccgtatcg
     3541 gtcgtacagt cgttcgtgca gctcgcgatc gagtcgtcgc agtcgggacc cgagcgagtc
     3601 gtgtaggcat cgcacccggt gaagtagagg tcggagtcgc cgtcgacgca
//
LOCUS       DASPGN010000333         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1463308, whole genome shotgun sequence.
ACCESSION   DASPGN010000333 DASPGN010000000
VERSION     DASPGN010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1463308"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /gene="selD"
                     /locus_tag="VD788_09605"
     CDS             <1..737
                     /gene="selD"
                     /locus_tag="VD788_09605"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HXV76563.1"
                     /translation="VGYPDDLDIAILGEILRGGGEKLREAGAVIAGGHTTTDREPKYG
                     IAALGLVHPERVLTKGGARPGDVLLLNKAIGTGVTTTAAKQQQADPTQLAGAVASMAR
                     LNGGASQVLSRFAEQVHAATDITGFGLAGHAHEMAHHSGLTLRLVWDRVPLLDGAAGY
                     ARGGFVPAGGKRNRSYYAPWVAVRRALADWEQVLLYDPQTSGGLLAAVAAERADEVER
                     AFGEAGEPVWRVGTAIEGRAGGLEIA"
     gene            complement(747..2714)
                     /locus_tag="VD788_09610"
     CDS             complement(747..2714)
                     /locus_tag="VD788_09610"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76564.1"
                     /translation="MNSRASLVLPAVIVVLTLGVYTPAFEAGFIWDDDDYVSENPLLR
                     DADGLLRIWTTTETPQYYPMVFTTFWLEHQLWGLAPAGYHVVNVLLHALNAILLGYAL
                     RLLSVPGAWWVAVLFAVHPVQVESVAWVTERKNVLSGLFYLLSSLSFLRFERDRRTRH
                     YLLSLATFVLALLSKTVTASLPVALGLALLWRNGRLRRADVLRLVPYVLLGAGLALIT
                     VGLESEMIRAVRSDFDLSWWQRLLIAGGALLFYPWKLVVPYPLIFNYPRWDLAAPAAL
                     YAALVVVCVVCVALSVAWRRGRRGVVLAALFYGATIFPVLGFLDVYPFRYSFVADHFQ
                     YLASVGPLIVLVGLGRASSRWLCRRAPAAGPAISRAAGAAIVAALALLGFNQASIYHD
                     LPTLWQHTIAHNPRSWLALHNMGLFRMNAGDLDAALSQLDRAIEAKPASAESYTARGL
                     TRYRAGDQDAALADLNRAVELQPSYPQARLNRGNVLLDLGRPDLAIADLEIFVDANRS
                     YLPARHSLARALLRMKRYAEAAAHLDEIVRRGPDYEAFLNRGVAQVNLERYERAVADF
                     TRALELRPGATDAWSNRAFAYVRLGRPVSALEDLDRSIALDGDNVRLFLMRGGVHQTL
                     GDMRRACMDWQQACARGDCRLLQQNCFRGGG"
     gene            complement(2745..>3253)
                     /locus_tag="VD788_09615"
     CDS             complement(2745..>3253)
                     /locus_tag="VD788_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76565.1"
                     /translation="SASPLNHSYQQQNRVLPFPFHFLDNNQAMNVRPRNYDWPEFYDR
                     LIDLCLYAFSWRAIGARLWANGSNIPGWMNVVRAVSSEGFGRIRHHRRVRQRLDDDRE
                     LRAYFDQRSTTLPEFYRARIARELGAFFRWLPAGAIDHDPNTFAYDESRPVVPRAGAI
                     ARAVARAE"
BASE COUNT          558 a         1098 c         1170 g          427 t
ORIGIN      
        1 tggtcggcta ccccgacgac ctcgacatcg cgatcctcgg cgagatcctg cgcggcggcg
       61 gcgaaaagct gcgcgaggcg ggggcggtca tcgccggcgg ccacacgacg accgaccgcg
      121 agccgaagta cggcatcgcc gcgctcggtc tggtgcaccc cgagcgggtg ctgaccaagg
      181 gcggcgcgcg ccccggcgac gtcctgctgc tgaacaaggc gatcggcacc ggcgtgacga
      241 cgacggcggc caagcagcaa caggccgatc cgacccagct ggccggggcg gtcgccagca
      301 tggcgcgtct caacggcggc gcatcgcagg tcctgtcgcg tttcgccgag caggtccacg
      361 cggcgaccga catcaccggg ttcgggctgg ccggtcacgc gcacgagatg gcgcatcatt
      421 ccgggttgac cttgcggctg gtgtgggacc gggtgccgct gctcgacgga gcggcaggct
      481 atgcccgcgg cggtttcgtc cccgccggcg gcaagcgcaa ccgctcgtac tacgcaccgt
      541 gggtggcagt gcgacgcgcg ctcgccgatt gggagcaggt cctgctctac gacccgcaga
      601 cctcgggcgg tctgttggcc gcggtcgccg cggagcgcgc cgacgaggtc gagcgcgcct
      661 tcggcgaggc cggagagccg gtgtggcggg tgggcacggc gatcgagggc cgtgccggcg
      721 ggctcgagat cgcctgagcg ccaacttcac ccaccgccgc ggaagcagtt ctgctgcagc
      781 aggcggcaat cgcctcgcgc gcacgcctgc tgccagtcca tgcaggcgcg tcgcatgtcg
      841 ccgagggtct ggtgcacgcc gccgcgcatc aggaacagcc gcacgttgtc gccgtcgagc
      901 gcgatcgacc gatcgagatc ctcgagcgcg gatacgggtc gcccgagccg tacgtaggcg
      961 aaggcgcggt tggaccacgc gtccgtcgcc ccgggacgca gctcgagcgc ccgggtgaaa
     1021 tcggcgaccg ctcgttcgta acgctcgaga ttgacctggg ccacgccgcg gttgagaaag
     1081 gcctcgtaat cggggccgcg ccgcacgatc tcgtcgagat gcgccgccgc ttccgcgtac
     1141 cgcttcatcc gcagcagcgc gcgcgccagc gagtgccggg ccggcaggta ggaccggttg
     1201 gcgtcgacga agatttcgag atcggcgatc gccaggtccg ggcgtcccag gtcgagcagt
     1261 acgttgccac gattcaaccg ggcctgcgga tacgatggtt ggagctcgac ggcacgattc
     1321 aggtcggcga gcgccgcgtc ctgatcgccg gcccggtagc gggtcaggcc gcgcgccgtg
     1381 tacgactcgg ccgacgcggg cttggcctcg atcgcccgat cgagctggct gagcgccgcg
     1441 tccaggtcgc cggcgttcat ccggaacagc cccatgttgt gcagcgccag ccacgagcgg
     1501 ggattgtgcg cgatcgtgtg ctgccacagc gtcggcaggt cgtggtagat cgacgcctgg
     1561 ttgaaaccga gaagagccag cgcggcgacg atcgccgccc ccgccgcgcg cgagatcgcg
     1621 gggccggctg ccggtgcacg ccggcagagc caccggctcg acgcgcggcc gagaccgacc
     1681 aggacgatca gcggtccgac gctcgcgagg tattggaagt ggtcggcgac aaacgagtag
     1741 cggaacgggt agacgtcgag aaagcccagc acgggaaaga tcgtcgcgcc gtagaacagc
     1801 gccgcgagga cgacccctcg ccggccgcgc cgccaggcga cgctgagggc gacgcagacg
     1861 acacacacga cgaccagcgc ggcgtagagc gccgccggcg cagcgagatc ccagcgcggg
     1921 tagttgaaga tcaacgggta gggcacgacg agcttccacg ggtagaacag caacgctccg
     1981 ccggcgatca gcagccgttg ccaccacgac aagtcgaagt cggagcgcac cgcgcgtatc
     2041 atctcgctct cgagcccgac ggtgatcaac gccagacccg cgccgagcag gacgtagggc
     2101 acgaggcgca gcacgtccgc gcgccgcagc cgtccgttgc gccacagcag cgcgagaccg
     2161 agcgcgacgg gcagactcgc ggtcaccgtc ttgctgagca acgcgaggac gaacgtggcg
     2221 agcgacagca ggtaatgccg cgtgcgccgg tcgcgctcga agcgcaggaa gctcagcgaa
     2281 gacagcagat agaacagacc cgacaggacg ttcttgcgct cggtgaccca ggcgaccgat
     2341 tcgacctgga ccgggtggac ggcgaacagg acggcgaccc accacgcacc gggcaccgac
     2401 agcagacgca gggcgtagcc gagcaagatc gcgttgagcg cgtgcagcag cacgttgacg
     2461 acgtggtagc cggccggtgc cagcccccac agctggtgct cgagccagaa ggtggtgaag
     2521 accatcggat agtactgcgg cgtttccgtc gtcgtccaga tccgcagcag gccgtcggcg
     2581 tcgcgcagca gtgggttctc gctgacgtag tcgtcgtcgt cccagatgaa accggcctcg
     2641 aacgccggtg tgtagacccc gagcgtcaag acgacgatca ccgccggcaa caccaacgac
     2701 gcgcgcgagt tcacggtcgg gatcctaacg cacgccgcgt gcgctcactc ggcgcgggcc
     2761 accgcccgcg cgatcgcacc ggcccgcggc acgacgggcc gactctcgtc gtaggcgaac
     2821 gtattcgggt cgtggtcgat cgcgccggcc ggcagccagc ggaagaacgc ccccagctcg
     2881 cgtgcgatcc gtgcgcggta gaactcgggc agcgtcgtgc tccgctgatc gaagtaggcg
     2941 cgcagctccc gatcgtcgtc gagacgttgc cgcacccgcc ggtggtgtcg gatgcggccg
     3001 aagccctcgg aggagacggc gcgcacgacg ttcatccagc ccgggatgtt gctgccgttg
     3061 gcccacagac gcgctccgat cgcgcgccag gagaacgcgt agaggcaaag atcgatcagc
     3121 cggtcgtaga actccggcca gtcgtagttc cgcggccgta cgttcatcgc ctggttgttg
     3181 tcgaggaagt ggaacgggaa cggcagcaca cgattctgct gctggtagct gtggttcaac
     3241 ggagaggcgc tgc
//
LOCUS       DASPGN010000334         9552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1581393, whole genome shotgun sequence.
ACCESSION   DASPGN010000334 DASPGN010000000
VERSION     DASPGN010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9552
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1581393"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VD788_09620"
     CDS             <1..298
                     /locus_tag="VD788_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXV76566.1"
                     /translation="IREENAVVLGVSPDGGPSHRGFRAKYELPFALLSDPDKRVMQQY
                     GAWGEKTLYGRKTVGVIRSTVLIGPDGTVVKHWKRVGQAADHPAQVLDALRAIG"
     gene            470..1846
                     /locus_tag="VD788_09625"
     CDS             470..1846
                     /locus_tag="VD788_09625"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HXV76567.1"
                     /translation="MQRKVDNMRIGRRGFSLITVGLFTVCCCGLVAAAALTPEQRLGK
                     ELFVDPISTPAQAVSCASCHAPQVGFVGPNTGINLHGGVYRGAVPTRFGNRKPPSAAY
                     ATLSPVFHFDDDEGLFVGGNFWDGRATGDRLGNPAADQAGGPFLNPVEQNMPDKQSVC
                     MHVAGARYADLFREVWGADSLDCSSAGVELTYDRIALSIAAYEDSPEVNAFSSKYDRW
                     LVGTAELTTQETWGLELFNGKAMCAACHISEVGPNGEKPLFTDYTYDNLGVPRNPENP
                     FYQMDTVYLDDGSAINPLGRDWVDYGLGEFLANQSNPDWAAMADESMGKQKVPTLRNV
                     AKVFGHRTPKAFGHNAYFKSLESIVHFYNTRDAKPVCPDPFTSEKDALAAGCWPAPEV
                     GMNVNITELGNLGLTAAEEAAVVAFLHTLSDGYLERGVSRNRAARPDEVQREAIDGGD
                     AARLPARR"
     gene            1963..3315
                     /locus_tag="VD788_09630"
     CDS             1963..3315
                     /locus_tag="VD788_09630"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HXV76568.1"
                     /translation="MKGWRILAAAATVVLALPLINLAVGLPRSPLAEIAATDPHPTAA
                     ARVIARKCVHCHTSQPELPFYARVPFAARLIDRDIREGSRRVDYLAGLTDAAAPSEVL
                     LAKTDRVIEQRTMPPTRFVMLHWGLDLTNEDRAALSAWTGSVRVTTYADPGTPAALRS
                     AAIRPLPAQVDADPRKVELGRRLFHDPRLSGDDSVSCASCHDLAKGGTDQRRYSIGVG
                     GARGGINSPTVFNASYNVLQFWDGRAGDLALQADGPVNNPIEMASNWEQVTSKLARDR
                     AFTAAFRQVYPDGYSPESVVDAIAEFERTLITPDSPFDRFLVGDDAALDDEQRRGWQL
                     FRDNGCATCHTGAALGGGSFEVMGLEADYFGDRGGPSETDFGRFNATGRESDRYRFKV
                     PTLRNVALTWPYFHDGSVCELDEAVRMMALYQRGRVLAEADVQAMVAFLESLTGVYRG
                     "
     gene            3357..5852
                     /locus_tag="VD788_09635"
     CDS             3357..5852
                     /locus_tag="VD788_09635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV76569.1"
                     /translation="MDLSHDRWRRADALFDAALDLPPAERDAFLDRECGEDRPLRRLV
                     GRLLGNDAPTVGFELTPDGVGRAPLLYPIDDGSSVEEADEVLPSGVVVDRYRIVRELG
                     RGGMAVVYLAERADGQFEQQVALKLIKRGTDTDQVVRRFAQERQMLAAVNHPNIAKLL
                     DGGATAEGRPYFVMEYIEGEPIDAFCDVRRLDVEQRLGLFLKVTDAVSHAHRNLVVHR
                     DLKPSNILVTGDGEVKLLDFGIARYVSDGGEDDPALTRTGVRVMTPAYASPEQVRGDP
                     VTTASDVYQLGLLLFRLLAGRSPYVIDGGSAESLQRAICQTAPPRPSATLAAAGGSTG
                     AKPDSADAISRRRGTTLAALKRRLAGDLDTVVLACLRKEPDRRYPSVAQLADDIDRHL
                     TGRPVTARPDTFGYRAGKFVRRHKLAVAFVSLVLAMLVTFSVVTTIQAGRIARERDRA
                     NREAAAAGQVADFLVDLFEIADPSEARGSSITAREILERGAAAIDDELTDQPLVQARM
                     KDAIGRVYRNLGLYDEAEPLLRQALEARRDNLGEDRLATAHSLHELGYLLELRGDLDG
                     AEAHYRRSLALYRELAGEDSVLAAVGMNNLAVVLHRKGDLDGAEPLYRRALAIRQARN
                     DDDVSNILSNLATIHQDRGRLDDAIELFRRALEFAEQRLGPDHPESVTTLNNLALALK
                     ESGDYDAAEPLYRRAVELDRRVLGERHPNFAVAVNNLADLLETKGELAEAETLYRQAK
                     EIWLAALGERHWLTALGRGNHGGVLARLGAYRAAEDELLAAIAGLEQALGPDHERTIK
                     AVRRLAELYEAQGRFAEAEPLRERLAAGGGDAT"
     gene            complement(5856..6200)
                     /locus_tag="VD788_09640"
     CDS             complement(5856..6200)
                     /locus_tag="VD788_09640"
                     /inference="COORDINATES: protein motif:HMM:NF020426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HXV76570.1"
                     /translation="MPNKRLYKIIFQSQGKVYELYAKKVAHGSLFGFVEIEEIVFGER
                     TQVVVDPSEEQLKTEFQGVRRTFLPIHAVVRIDEVEKQGVARISPGSGNDGVVKPFPT
                     PIPVPPPRDGKG"
     gene            6281..6724
                     /locus_tag="VD788_09645"
     CDS             6281..6724
                     /locus_tag="VD788_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76571.1"
                     /translation="MPRALLPFVIVLAAAGCAGSETPDGAPAAAAAADCVERVLRQDG
                     ELAAVRNHAPKQMPLADAVGNYVAGLAGLDFSGCPAGFTDAFARHRSAWFDAVEFLGS
                     YDTMRGEMHDLFEQIRAESPDAAARLSAHEQAIWSTWAEVESAGR"
     gene            6868..7938
                     /gene="had"
                     /locus_tag="VD788_09650"
     CDS             6868..7938
                     /gene="had"
                     /locus_tag="VD788_09650"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: protein motif:HMM:TIGR03201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HXV76572.1"
                     /translation="MNLEAWVVEEPGRPMARRRREVTAGPGEVVIEVAGCGLCHTDLG
                     FYYDAVPTRHPFPLTLGHEIAGRVVETGEGAEEWRSCAVIVPAVIPCGACAACAAGRS
                     SICPEQIFPGNDVHGGFATHVVVPSRGLCRVPDLRDPRRNPLGVELAQLSVIADAVST
                     PWEAIRRSGLASGDLAVFVGAGGVGGFGVQLAAAVGAAVVAIDVDERRLERLAGCGAS
                     LVLNAAIGFRELKRAVRDFADRHDAPSWRRKIFETSGTAAGQATAFGLLEHGGHLAVV
                     GFTPERVELRLSNLMAFDATAQGNWGCPPEHYPALVDLALSGKIALQPFTERRPLSSI
                     NEAFADVHAHRVDRRVILSPER"
     gene            7941..9044
                     /gene="oah"
                     /locus_tag="VD788_09655"
     CDS             7941..9044
                     /gene="oah"
                     /locus_tag="VD788_09655"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018807 -
                     6-hydroxycyclohex-1-ene-1-carboxyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HXV76573.1"
                     /translation="MELKNHDLVDRTEFDGVRYDTVPLRDPDRKTIAGLHVARITLDN
                     PPQLNSYTTTMVKGVIIGMRRASNDRACVGVVFSGTGTRAFCTGGNTAEYAEYYAGRP
                     EEYRQYMRLFNDMVTAILHCDKPVICRVNGMRIAGGQEIGMACDFSVAQDLSLFGQAG
                     PRHGSAPDGGSTDFLPLFVGVEAAMESCTLCEQWSAHKALRMGLLTRITPALRVDGEW
                     IANPLVVTDRWIDERGRIVYGERKQGDELAAGKRLLERGEIDLSLLDAEVDGLIWSLA
                     HTMPGCLTKTIESVRKHKLAWWDRNRETNRAWLGLNMMTEGQAGFRAFHEGTRECREA
                     DFLLLRRSLAEGQVWGDELVERVLARAHGKVTS"
     gene            9041..9520
                     /locus_tag="VD788_09660"
     CDS             9041..9520
                     /locus_tag="VD788_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV76574.1"
                     /translation="MTIRLGEPGCAIAVRDLRRSDLADVIRIDAAHTGAAKPEYWDRV
                     LREFLDPRRERTAVGLATDDPIGLSGYLLGEVRAFEFGSPACGWVFAIGVDPARTRRR
                     IASLLLAECCRRFARAGIACVRTMVLRDNVPVLSFFRDCGFVAGSFVQLELDLGEDR"
BASE COUNT         1480 a         3266 c         3352 g         1454 t
ORIGIN      
        1 gatccgggaa gagaacgcgg tcgtgctcgg ggtctcgccc gacggcggcc cgtcgcaccg
       61 ggggttccgc gccaagtacg agcttccgtt cgccctgttg agcgatccgg acaagcgggt
      121 gatgcagcag tatggcgcct ggggcgaaaa gacgctgtac ggcaggaaga ccgtcggcgt
      181 catccgttcg acggtgctga tcggacccga cgggacggtc gtcaagcact ggaaacgcgt
      241 gggccaggcg gccgaccatc cggcgcaagt gctcgacgcg ttgcgcgcca tcggctgacg
      301 ccccgcggcg ccggccgatc ctccgacgat cgcaccggcc tatcacacca tcgggacccc
      361 aacggtcgtg tacgcgatct tgcgccaccc gtcgccgcac cgcgttaatg accccagtcg
      421 gccagaggtg tcgctctcct ggggtctcga ggatcggcgc ggacggccaa tgcaacgaaa
      481 ggtcgacaac atgagaatcg gacgacgcgg cttttctctc atcaccgttg gtctgttcac
      541 cgtctgctgc tgcgggctgg tcgcggcggc cgccctgacg ccggagcaac ggctcggcaa
      601 ggagctgttc gtcgacccga tctcgacacc ggcacaggcc gtgtcgtgcg cgagctgtca
      661 cgccccgcag gtcggcttcg tcggcccgaa cacggggatc aacctgcacg ggggcgtcta
      721 ccgcggcgcc gtcccgacgc gcttcggtaa ccgcaagccg ccgagcgcgg cctacgccac
      781 gctcagcccg gtgttccact tcgatgacga cgaaggtctg ttcgtcggcg ggaacttctg
      841 ggacggtcgt gccaccggcg atcggctggg caaccccgcc gccgaccagg ccggcggccc
      901 gttcctcaat ccggtcgagc agaacatgcc cgacaagcag tcggtctgca tgcacgtggc
      961 gggcgcgcgc tacgccgacc tgttccgcga agtgtggggc gccgattccc tcgactgcag
     1021 cagcgcgggc gtcgagctga cctacgaccg gatcgcgctg tcgatcgcgg cctacgagga
     1081 ctcgcccgag gtcaacgcgt tctcgtcgaa gtacgatcgc tggctcgtcg gcacggccga
     1141 gctgaccacg caggaaacat gggggctcga gctgttcaac ggcaaggcga tgtgcgccgc
     1201 ctgccacatc agcgaagtgg ggccgaacgg cgagaagccg ctgttcaccg actacacgta
     1261 cgacaacctg ggcgtgccgc gcaacccgga gaatccgttc taccagatgg acacggtcta
     1321 tctcgacgac ggctcggcga tcaacccgct gggacgcgac tgggtcgact acggattggg
     1381 cgagttcctc gcgaaccaat cgaaccccga ctgggcggcg atggccgacg agagcatggg
     1441 caagcagaag gtcccgacgc tgcgcaacgt ggccaaggtg ttcggccatc gcacgccgaa
     1501 ggccttcggg cacaacgcct acttcaagtc gctcgagagc atcgtgcatt tctacaacac
     1561 gcgtgacgcc aagccggtct gtcccgaccc gttcaccagc gaaaaggacg cgctggccgc
     1621 cggctgctgg ccggcgcccg aggtcgggat gaacgtcaac atcaccgagc tcggcaacct
     1681 gggcttgacc gcggccgagg aggcggcggt cgtggccttc ctgcatacgc tgtcggacgg
     1741 ctacctggaa cgcggtgttt cgcggaaccg cgccgcccga ccggacgagg tccagcgcga
     1801 agcgatcgac ggcggcgacg ccgctcggct tcccgctcgt cgctgagcgg ccgaagggcc
     1861 gggccgggtc gcgcgcggac ccggccccgc cccgtaccac gacaaaacac ggggtcgaac
     1921 accatgacga cgcagcccgc agcgaaacgg acccgacgaa gcatgaaggg atggcggatc
     1981 ctcgccgccg cggcgaccgt ggtcctcgcg ttgccgctga tcaacctcgc ggtcggcctg
     2041 ccgcgaagcc cactcgccga gatcgcggcg accgacccgc atccgaccgc cgcggcacga
     2101 gtcatcgcac gcaagtgcgt gcactgccac acgtcccaac cggagcttcc gttctacgcg
     2161 cgcgtgccgt tcgccgcacg gctgatcgac cgcgacatcc gcgagggatc gaggcgcgtc
     2221 gactatctcg ccggcctgac cgacgccgcg gcgccgagcg aggtgctgct ggccaagacc
     2281 gaccgggtga tcgagcagcg gacgatgccg ccgacgcggt tcgtgatgct gcactggggt
     2341 ctcgacctga cgaacgagga tcgcgccgcg ttgagcgcgt ggaccggctc ggtgcgcgtg
     2401 acgacctacg ccgaccccgg cacgcccgcg gcgttgcgct cggcggcgat ccgtcccctg
     2461 cccgcgcaag tcgacgcgga cccgcgcaag gtcgagctcg gccggcggtt gttccacgac
     2521 ccgcgtctgt cgggcgacga ttcggtctcc tgcgcctcgt gtcacgatct cgccaagggc
     2581 ggaaccgatc agcgccggta ctcgatcggc gtcggtggag cgcgcggcgg gatcaactcg
     2641 ccgacggtgt tcaacgcgtc gtacaacgtg ctccagttct gggacggccg cgcaggcgat
     2701 ctggcgctgc aagccgacgg gccggtcaac aacccgatcg agatggcgtc gaactgggaa
     2761 caggtgacga gcaagctggc gcgggaccgt gcgttcaccg cggcgtttcg ccaggtgtac
     2821 cccgacggat acagcccgga gagcgtcgtc gacgcgatcg cggagttcga acggacgttg
     2881 atcacgccgg attcgccgtt cgaccggttc ctcgtcggtg acgacgccgc gctcgacgac
     2941 gagcagcggc ggggctggca gctattccgc gacaacggct gcgcgacctg ccatacgggc
     3001 gccgcgctcg gcggcggctc gttcgaagtg atgggcctcg aggccgacta cttcggcgat
     3061 cgcggcgggc cgtccgagac cgatttcggc cggttcaatg cgaccggtcg ggaatccgac
     3121 cgttatcgct tcaaggtccc gacgttgcgc aatgtcgcgc tgacctggcc ctacttccac
     3181 gacggatcgg tttgcgagct cgacgaggcg gtgcggatga tggcgctcta tcagcgcggg
     3241 cgagtgctgg ccgaggcgga cgtgcaggcg atggtcgcgt tcctcgagtc gctgaccggc
     3301 gtctaccggg gatagcgcct gtcgccgagc ggtgacctgg tagaatcgcc gcgacgatgg
     3361 acctgtcgca tgatcggtgg cgtcgcgccg acgcgttgtt cgatgccgcg ctcgatcttc
     3421 ctcctgccga acgcgacgcg ttcctcgatc gggaatgcgg cgaggaccga ccgctgcgcc
     3481 ggctcgtcgg gcgactgctg ggcaacgacg cgccgaccgt cggtttcgag ctcacgccgg
     3541 acggcgtggg tcgcgctccc ctgctctacc cgatcgacga cgggtcatcg gtcgaggagg
     3601 cggacgaggt cctgccgagc ggagtggtcg tcgaccgcta ccggatcgtg cgcgagctgg
     3661 gccgtggcgg gatggccgtg gtctacctcg ccgagcgcgc cgacggccaa ttcgagcagc
     3721 aggtcgcgct caagttgatc aagcgcggga cggacaccga ccaggtcgtc cgccggttcg
     3781 cccaagaacg ccagatgctg gccgcggtga accacccgaa tatcgccaag ctgctggacg
     3841 gcggcgcgac cgccgaaggc cgaccgtact tcgtgatgga gtacatcgag ggagaaccga
     3901 tcgacgcgtt ttgcgacgta cgtcggctcg acgtcgaaca gcgcctcggg ctgtttctca
     3961 aggtcaccga tgcggtgtcc cacgcacacc gcaacctggt cgtgcaccgc gacctcaagc
     4021 cgtcgaacat cctcgtcacc ggtgacggcg aggtcaagct gctcgacttc ggcatcgcgc
     4081 gttacgtcag cgacggcggc gaggacgacc cggccttgac ccgcaccggc gtccgcgtga
     4141 tgacgccggc ctacgccagc ccggagcagg tccgtggcga cccggtgacg acggcctccg
     4201 acgtctacca gctggggctg ctgctgttcc ggctgctggc cgggcgctcg ccgtacgtga
     4261 tcgacggagg ttcggccgag tcgctccagc gcgcgatctg ccagacggcg ccgccgcgac
     4321 cgagcgccac cttggccgcg gccggcggct cgaccggcgc gaaaccagat tcggcggatg
     4381 cgatcagccg gcgccgtggc acgacgctcg cggctctgaa acgcaggctg gccggtgacc
     4441 tcgataccgt cgtgctggcg tgcctgcgca aggagccgga ccggcgctac ccgtccgtcg
     4501 cacagttggc cgacgacatc gaccgtcatc tcaccggccg tccggtgacc gcgcggccgg
     4561 acaccttcgg ctaccgcgcg ggcaagttcg tccgccgcca caaactcgcc gtggccttcg
     4621 tctcgctggt cctggcgatg ctggtgacgt tttccgtcgt gacgacgatc caggccgggc
     4681 ggatcgcgcg cgagcgcgac cgggccaacc gggaggcggc cgccgccggc caggtcgccg
     4741 actttctcgt cgacctgttc gagatcgccg acccgagcga agcgcgcggc agctcgatca
     4801 cggcccgcga gatcctcgag cgcggtgctg cggcgatcga cgacgagctg accgatcaac
     4861 cgctggtgca ggctcggatg aaggacgcaa tcggtcgcgt ctaccgcaac ctcggcttgt
     4921 acgacgaggc cgagccgttg ctgcgccaag cgctcgaagc caggcgcgac aatctcggcg
     4981 aggaccggct cgccacggcg cacagcctgc acgagctcgg ctatctgctc gagctgagag
     5041 gggacctcga cggggccgaa gcgcactacc gtcgttcgct cgcgctctac cgcgagctgg
     5101 ccggcgagga cagcgtgctg gccgcggtcg ggatgaacaa cctggccgtc gtcctgcacc
     5161 gcaagggcga cctggacggc gccgagccgc tctatcgcag ggcgctggcg atccgccagg
     5221 cacgcaacga cgacgacgtc tcgaacatcc tgagcaacct ggcgacgatc caccaggacc
     5281 gtggccggct cgacgacgcg atcgagctgt tccgccgagc gctcgagttc gccgagcagc
     5341 gactggggcc ggaccatccg gaaagcgtga cgacgctgaa caacctggcc ctcgcgctga
     5401 aggagtccgg tgactacgac gccgcggagc cgctttaccg gcgggcggtg gagctcgacc
     5461 ggcgcgtcct cggcgagcga caccccaact tcgcggtggc ggtcaacaac ctggccgacc
     5521 tgctcgagac gaagggcgaa ctggcggagg ccgagacgct ctaccgccaa gccaaggaga
     5581 tctggctcgc cgccctcggc gagcggcatt ggctgacggc gctcggccgc ggcaaccacg
     5641 gtggtgtgct ggcgcgcctg ggcgcctatc gcgcggccga agacgagctg ctggcggcga
     5701 tcgccgggct ggagcaggcg ttggggccag accacgagcg cacgatcaag gccgtgcgca
     5761 ggttggccga gctttacgag gcgcagggac gattcgccga agccgagccg ctgcgcgagc
     5821 gtctggcggc cggtggcggc gacgcgacct gaggctcaac cctttccgtc gcgcgggggc
     5881 gggaccggta tcggcgtcgg gaacggcttg accacgccgt cgttgccgga tcccggcgag
     5941 atgcgggcga caccctgctt ttcgacctcg tcgatgcgaa cgacggcatg tatcggcagg
     6001 aatgtccggc ggaccccctg aaactccgtc ttcagctgct cttcggacgg atcgacgacg
     6061 acctgggtcc gctcgccgaa gacgatctcc tcgatctcga cgaagccgaa cagcgagccg
     6121 tgggcgacct tcttcgcgta caactcgtag accttgccct gactctggaa gatgatcttg
     6181 tacagtcgtt tgttcggcac cgtcactccc gcccccgatc ggccgtcgtc gggtcgcggg
     6241 tattctagcc ggtcagtcgg gatcgtgtgg agtttcgacc atgccgcgag cactgctgcc
     6301 gttcgtgatc gtcctcgccg ccgccggttg cgccggcagc gaaacgccgg atggcgcccc
     6361 ggcggcggcc gcggcggcgg actgcgtcga gcgggtcctg cgtcaggacg gcgagttggc
     6421 ggcggtgcgg aaccacgcgc cgaaacagat gccgctggcc gatgccgtcg gcaactacgt
     6481 cgcggggctg gccgggctcg acttcagcgg gtgtcccgcc gggttcaccg acgcgttcgc
     6541 ccgccaccgg tccgcctggt tcgacgcggt cgagttcctc ggttcctacg acacgatgcg
     6601 cggcgaaatg cacgacctgt tcgagcagat ccgcgccgaa agccccgacg ccgcggctcg
     6661 actctccgcg cacgagcagg cgatctggag cacgtgggcc gaggtcgagt ccgccggtcg
     6721 ctgaggtctt gcccgacgcc gtccaagcga cattcgggac ctcttttgtg ttcgaccgcg
     6781 cgttgaccgg gacccctcgt ccgcgctgat attgggaaat cggagtctga ttaccgaatc
     6841 gcgcaggacg cgcggagacg catcgcgatg aacctcgaag cgtgggtcgt cgaagaaccg
     6901 ggccggccga tggcccgtcg ccggcgcgag gtgaccgcgg gacccgggga ggtcgtgatc
     6961 gaggtcgcgg gctgcggcct gtgtcacacc gatctcggtt tctactatga cgccgtgccg
     7021 acgcgtcacc ccttcccgct gaccctcggc cacgagatcg ccggtcgcgt ggtcgagacg
     7081 ggcgaggggg ccgaggaatg gcggtcgtgc gcggtgatcg tacccgccgt gatcccctgc
     7141 ggcgcgtgcg cggcgtgcgc ggccgggcgc agctcgatct gccccgagca gatattcccc
     7201 ggcaacgacg tgcacggtgg tttcgcgacg cacgtcgtcg tgccgagccg cggcttgtgt
     7261 cgggtccccg acttgcgcga cccgcggcgg aacccgctcg gtgtcgagct ggcacagctg
     7321 tcggtcatcg ccgatgcggt ctccacgccg tgggaggcga tccgccggtc gggactggcc
     7381 agcggcgacc tcgcggtctt cgtcggcgcc gggggagtcg gcgggttcgg cgtgcagctc
     7441 gccgcggcgg tcggcgccgc ggtcgtcgcg atcgacgtcg acgagcgacg gctcgagcgg
     7501 ctcgccggtt gcggggcctc gctcgtcttg aacgcggcga tcggctttcg ggagctgaag
     7561 cgcgccgtac gggacttcgc cgaccggcac gacgcgccga gctggcggcg gaagatcttc
     7621 gagacctcgg gcaccgccgc cggccaggcg acggcgttcg ggctgctcga gcacggcggt
     7681 cacctggccg tcgtcggctt cacgccggag cgggtcgaac tgcgcctgtc gaacctgatg
     7741 gcgttcgacg cgacggcgca gggcaactgg ggctgcccgc ccgagcacta cccggcgctg
     7801 gtcgacctcg ctctgtcggg caagatcgcc ctgcagccgt tcaccgaacg acgaccgttg
     7861 tcgtcgatca acgaggcgtt cgccgacgtt cacgcgcacc gcgtcgatcg gcgggtgatc
     7921 ctttctccgg agagatgacg atggagctca agaaccacga tctcgtcgac cgcaccgagt
     7981 tcgacggcgt gcgctacgac accgtgccac tgcgcgatcc cgaccgcaag acgatcgccg
     8041 gcctgcacgt ggcgcgcatc acgctcgaca acccgcctca gctcaactcc tacacgacga
     8101 cgatggtcaa aggggtgatc atcggcatgc gtcgcgcctc gaacgatcgg gcctgcgtcg
     8161 gggtggtctt cagcggcacg ggcacccggg cgttctgcac cggcggcaac accgccgagt
     8221 acgccgagta ctacgcgggg cgccccgagg agtaccggca gtacatgcgg ttgttcaacg
     8281 acatggtcac cgcgatcctg cactgcgaca aaccggtgat ctgccgcgtc aacggcatgc
     8341 gaatcgccgg cggacaggag atcggcatgg cctgcgattt ctccgtggcg caggatctca
     8401 gcctgttcgg acaggccggc ccgcgccacg gctccgcgcc ggacggcgga agcaccgact
     8461 ttctgccgct gttcgtcggc gtcgaggcgg cgatggagag ctgcacgctg tgcgagcagt
     8521 ggagcgcgca caaggcgctc cggatgggcc tgctgacacg catcactccc gcgctgcgcg
     8581 tcgacggcga gtggatcgcg aacccgttgg tcgtcaccga ccgctggatc gacgagcgcg
     8641 gccgcatcgt ctacggcgag cgcaagcagg gtgacgagct cgccgcgggc aagcggttgc
     8701 tcgagcgcgg cgagatcgat ctgtcgctgc tcgacgccga ggtcgacggg ctgatctggt
     8761 cgctcgccca tacgatgccc ggctgcctga cgaagacgat cgagagcgtg cgaaagcaca
     8821 agctcgcctg gtgggaccgc aaccgagaaa ccaaccgcgc ctggctcggg ttgaacatga
     8881 tgaccgaggg acaggccgga ttccgtgcgt ttcacgaagg aacccgcgag tgtcgggagg
     8941 ccgatttcct cctgctgcgc cggtcgctgg ccgagggaca agtgtggggt gacgagctgg
     9001 tcgagcgggt gttggcccgc gcccatggca aggtgacctc gtgacgatcc ggctcggcga
     9061 gcccgggtgc gcgatcgcgg tgcgcgactt gcgccgctcg gacctggccg acgtgatccg
     9121 catcgacgcc gcgcacaccg gtgcggccaa gcccgagtat tgggacaggg tgctccgcga
     9181 gtttctcgac ccgcggcgcg agcgcacggc ggtcgggctt gccaccgacg acccgatcgg
     9241 cctgtcgggc tatctgctgg gcgaagtgcg ggcgttcgag ttcggctcgc ccgcctgcgg
     9301 ctgggtcttc gcgatcggcg tcgatccggc gcgcacgcgg cgccgcatcg cctcgttgct
     9361 gctcgccgag tgctgtcggc gattcgcgcg cgccgggatc gcgtgcgtgc gcacgatggt
     9421 gctgcgcgac aacgtcccgg tgctgtcgtt ttttcgcgat tgcgggttcg tcgccggcag
     9481 cttcgtccaa ctggagctgg atctcgggga ggaccgatga accacgacgc ggcgctgcgt
     9541 tgcgagccga tc
//
LOCUS       DASPGN010000335         4107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_59444, whole genome shotgun sequence.
ACCESSION   DASPGN010000335 DASPGN010000000
VERSION     DASPGN010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59444"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VD788_09665"
     CDS             <1..513
                     /locus_tag="VD788_09665"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HXV76575.1"
                     /translation="VLADETRPFLQGSRLTAWELARDRIDTTLITDNMAGALMSSGEV
                     DLVVVGSDRIAANGDVANKIGTYTIAVLAKENGIPFYVAAPMSTIDFDCPDGSRIPIE
                     ERDSREVTEWFGTRLAPDGVGARNIAFDVTPARYVAAIVTERGIARPPFSESLRRLRE
                     AEAGDGDDRR"
     gene            510..1535
                     /gene="tsaD"
                     /locus_tag="VD788_09670"
     CDS             510..1535
                     /gene="tsaD"
                     /locus_tag="VD788_09670"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXV76576.1"
                     /translation="MTRILGIETSCDETAAAVLSDRHRVESSVVASQVELHSKYGGVV
                     PEIAARHHVEAIDPVIRQALNDAGVSLDQIDAVAVTRGPGLIGSLLVGVSAAKAIAWF
                     RQLPLVGVNHLEGHVRSVFIEHPRIEFPAIALVVSGGHTSLYLCPRESVYREVARTRD
                     DAAGEAFDKVAKLLGLGYPGGPVIERLSRGADERAYVFPQAQMKDGSLDFSFSGLKTA
                     VRRAAQAAGLVRPAAVGGEVTPVVCDLVASFQRAVVRVLVRQTMAVCDREQVRNVLVT
                     GGVACNGRLREEFARAAERRKLRLYFPSPRYTTDNAAMIAAAGFLHLEAGRLADNGLT
                     ADSSWKL"
     gene            complement(1542..2318)
                     /locus_tag="VD788_09675"
     CDS             complement(1542..2318)
                     /locus_tag="VD788_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HXV76577.1"
                     /translation="MKAIDLTGKTALVLGVANQRSLAWAIAESLGDAGAELAFTYQGE
                     RLQKNVQGLASRFPDAPVMACDVTSEEQVEGVFARVERDLGRLDILIHSIAFAPREAL
                     EGDFIETSLEGWRIALEISAYSFIHLARHALPLMQEKGGAMLALTYLASQRVVPRYNV
                     MGAAKAALEHGVRQLAAELGPRGIRVNAISAGPVATLSARGISGFSNMSAHHRERAPL
                     RRNIEAREIGDAALFLCSDLASGITGEILFVDAGYNIMAL"
     gene            2435..3451
                     /locus_tag="VD788_09680"
     CDS             2435..3451
                     /locus_tag="VD788_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV76578.1"
                     /translation="MRNRPEAGLMSGLLSIEDLHVRFEGAAGPVRAVDGLSFTVERGE
                     TYGLVGESGCGKSLTALAILRLTEPGRITAGAIRFDGHDLLGLSEKQMRAVRGARIGI
                     VFQEPAAALNPVMRVGAQVVEAIRVHRKVGRREAWREAVRLLGMVSLSDPAAQARAYP
                     HELSGGMKQRAMLAIALACRPSLLIADEPTTALDVTIQAQILELLRRLRREMELTVLL
                     ISHDLGVVAENADRVGVMYAGRIVEEAPAAGLFADPRHPYTRGLLAAIPHGRAGGGKR
                     RLATLPGQVPDPSALPVGCRFHPRCPERFEPCDRDYPAESHATPGRRVACHLYECAGV
                     PRAR"
     gene            3454..>4107
                     /locus_tag="VD788_09685"
     CDS             3454..>4107
                     /locus_tag="VD788_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HXV76579.1"
                     /translation="MADTGRALVEARDLRKTFRRRDSLVGAVTASVHAVNGVDLDVAR
                     AETIGLVGESGSGKSTLGRMLIRLIDPDHGSLRFDGIDLLALKPAELRALRRQFQIVF
                     QDAHGSLNPRMRVGAAIAEPLVVHRLVRSRSERRERVARLLEQVGLDASAGDRYPHEF
                     SGGQRQRIGIARAVACSPRFIVADEPVSALDPPVQAQIINLMLDLQRQLGLAYLFISH
                     "
BASE COUNT          585 a         1429 c         1454 g          639 t
ORIGIN      
        1 gtgctggccg acgagacgcg accgttcctc cagggctcgc gtctgacggc gtgggagttg
       61 gcgcgcgatc ggatcgacac gacgctgatc accgacaaca tggccggcgc gctgatgagc
      121 tcgggcgagg tcgacctggt cgtcgtcggc tcggaccgca tcgcggccaa cggcgacgtc
      181 gcgaacaaga tcggcaccta caccatcgcc gtgctggcca aggagaacgg cataccgttc
      241 tacgtcgccg cgccgatgtc gacgatcgac ttcgactgtc cggacggcag ccgtatcccg
      301 atcgaagagc gcgactcgcg cgaggtgacc gagtggttcg gcacgcgcct ggcgccggac
      361 ggtgtcggcg cgcgcaacat cgcgttcgac gtcaccccgg ctcgttacgt cgcggcgatc
      421 gtcaccgaac gcgggatcgc gcgccccccg ttcagcgagt cgctgcgccg cctgcgcgag
      481 gccgaggcgg gcgacggcga cgaccggcga tgacccgcat cctcggcatc gagacctcgt
      541 gtgacgagac cgcggcggcg gtgctgtccg atcgtcatcg cgtcgaatcg tcggtcgtcg
      601 cctcgcaggt cgagctgcac agcaagtacg gcggcgtcgt ccccgagatc gccgctcggc
      661 accacgtcga ggcgatcgac ccggtgatcc gccaagcgct gaacgacgcc ggcgtctcgc
      721 tcgaccagat cgacgcggtg gcggtgacac gcggaccggg gctgatcggc tcgttgctgg
      781 tcggcgtgtc ggcggccaag gcgatcgcct ggttccgaca gttgccgctg gtcggggtca
      841 accacctcga ggggcacgtc cgctcggtct tcatcgagca cccccggatc gaattcccgg
      901 cgatcgcgct ggtggtgtcg ggcggccaca cctcactcta cctctgcccg cgcgagagcg
      961 tctatcgcga agtcgctcgc acgcgcgacg atgcggcggg cgaggcgttc gacaaggtgg
     1021 cgaagctgct gggtctcggc tacccgggcg gcccggtcat cgagcgattg tcgcgcggcg
     1081 ccgacgagcg ggcctacgtc ttcccgcagg cccagatgaa ggacggctcg ctcgactttt
     1141 cgttcagcgg gctcaagacc gcggtgcgcc gcgcggcgca ggcggcgggc ctggtacgac
     1201 cggcggcggt cggcggcgag gtcacgccgg tcgtgtgtga tctggtcgca tcgttccagc
     1261 gggcggtggt gcgcgtcctg gtgcgccaga cgatggccgt ctgcgaccgc gagcaggtgc
     1321 gcaacgtgct ggtcaccggc ggtgtcgcct gcaacggccg gctgcgcgaa gagttcgccc
     1381 gcgccgcgga gcggcgcaag ctgcggctct attttccgtc gccgcggtac acgaccgaca
     1441 atgcggcgat gatcgcggcc gccgggttcc tgcatctcga agccggccgg ctggcggaca
     1501 acgggctgac tgcggattcg tcgtggaagc tgtgaccgcc gctacagcgc catgatgttg
     1561 tagccggcgt cgacgaacag gatctcgccg gtgatgccgg aggccaggtc gctgcagagg
     1621 aacaatgcgg cgtcgccgat ctcgcgagcc tcgatgttgc gccgcagcgg tgcgcgctcg
     1681 cggtggtgcg ccgacatgtt ggagaagccg ctgatcccgc gtgccgaaag cgtcgccacc
     1741 ggtccggccg agatcgcgtt gacccgaatg ccgcgggggc cgagctcggc cgcgagctgc
     1801 cgcactccgt gctcgagcgc cgccttggcc gcacccatca cgttgtagcg cggtacgacg
     1861 cgctggctcg ccaggtaggt cagcgccagc atcgcgcccc ccttctcctg catcagcggg
     1921 agggcgtgac gagccaggtg gatgaaggaa taggcgctga tctcgagcgc gatgcgccag
     1981 ccctcgagcg acgtttcgat gaagtcaccc tcgagcgcct cgcgcggcgc gaacgcgatc
     2041 gagtggatca ggatgtcgag gcggcccaga tcgcgctcga cgcgcgcgaa caccccctcg
     2101 acctgttctt cgctcgtcac gtcgcacgcc atcaccggcg cgtcggggaa tcggctggcg
     2161 agcccttgga cgttcttctg caggcgctcg ccctgatacg tgaaggccag ctcggcgccc
     2221 gcgtcaccga gcgactccgc gatcgcccag gccagcgaac gttggttggc cacgccgagc
     2281 accagagcgg tcttgccggt caaatcgatc gccttcacct gtcggacctc cccggcgccc
     2341 gcacgcggcg cggcacccca atagactaac ccgggacggc ccagaatatc gacgcccgtc
     2401 ccccggccgg tcaaccggcg ccggccgata tagaatgcgc aaccggccgg aggccgggct
     2461 gatgagcggc ctgctgtcca tcgaagatct ccacgtgcgg ttcgaaggag ccgcggggcc
     2521 ggtccgcgcg gtcgacggtt tgtcgttcac cgtcgagcga ggcgagacct acggtctcgt
     2581 cggtgaatcc ggttgtggca agagcctgac cgcgctcgcc attctgcgac tgaccgagcc
     2641 gggtcggatc accgccggcg cgattcgctt cgacgggcac gatctgctcg gtctgagcga
     2701 gaagcagatg cgcgcggttc gtggcgcgcg catcggcatc gtctttcagg aaccggcggc
     2761 ggcgctcaac ccggtgatgc gcgtcggcgc gcaggtggtc gaggcgatcc gcgtgcatcg
     2821 caaggtcggt cgacgcgagg cctggcggga ggcggtgcga ttgctcggga tggtctcgct
     2881 gtccgatccc gcggcacagg cgcgcgccta cccgcacgag ctgtcgggcg ggatgaagca
     2941 gcgcgcgatg ctggcgatcg cgctcgcctg ccggccgtcg ttgttgatcg ccgacgagcc
     3001 gacgaccgcg ctcgacgtga cgatccaggc gcagatcctc gaattgctcc ggcgcctgcg
     3061 ccgggagatg gagctgacgg tgctgttgat cagccacgac ctcggcgtcg tcgcggagaa
     3121 tgcggaccgc gtcggcgtga tgtacgccgg ccgaatcgtc gaggaggcgc cggccgcggg
     3181 gttgttcgcc gacccgcgcc atccctacac ccgcggcctg ctcgcggcga tcccgcacgg
     3241 cagggccggc ggcggcaaac gccggctggc gacgctgccc gggcaggtac ccgacccgtc
     3301 ggcgctgccc gtcggctgcc gcttccaccc gcgttgcccc gagcggttcg agccgtgcga
     3361 ccgcgactac ccggccgagt cgcacgccac gcccggccgg cgggtcgcct gccacctcta
     3421 cgagtgcgcc ggcgtgccgc gggcgaggta gcgttggccg acaccggccg agcgttggtc
     3481 gaggctcgcg acctgcgcaa gacgtttcgc cggcgcgatt cgctcgtcgg agccgtgacc
     3541 gcctcggtcc acgcggtcaa cggcgtcgat ctcgacgtcg cccgggcgga gacgatcggg
     3601 ctggtcggcg agtcgggctc gggcaagtcg acgctcggac ggatgttgat tcggctgatc
     3661 gatcccgacc acggctcgtt gcgtttcgac ggcatcgacc tgctggcgtt gaagcccgcc
     3721 gagctgcgcg cgctgcggcg acagttccag atcgtgttcc aggacgccca cggctcgctc
     3781 aacccgcgca tgcgcgtcgg cgcggcgatc gccgaaccgc tcgtcgtcca tcgtctcgtc
     3841 cggtcgcgca gcgaacgtcg cgaacgcgtg gcgcgcttgc tcgagcaggt cggactcgat
     3901 gcctccgccg gcgacaggta tccccacgag ttctccggcg ggcaacggca gcgcatcggc
     3961 atcgcgcgcg ccgtcgcctg ttccccgcgg ttcatcgtcg ccgacgaacc ggtgtccgcg
     4021 ctcgatccgc cggtgcaagc gcagatcatc aacttgatgc tcgacctgca acgccagctg
     4081 ggtctggcct atctgttcat ttcgcac
//
LOCUS       DASPGN010000336         2363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_411311, whole genome shotgun sequence.
ACCESSION   DASPGN010000336 DASPGN010000000
VERSION     DASPGN010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411311"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VD788_09690"
     CDS             complement(<1..435)
                     /locus_tag="VD788_09690"
                     /inference="COORDINATES: protein motif:HMM:NF014418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HXV76580.1"
                     /translation="MPRSVRLADPDDLARQVRRAAAAVDSARALLLDVDGTLAPIAPT
                     PEEATVPAGTLRALARLVDGGWTVAPVSGRPLGQVRRMLPLDGLLRFGSHGLEGEAYP
                     GGSAPEVEAGARRLLDRLTRDAAEVVDGFPAVRVEAKPCGLAF"
     gene            complement(649..2055)
                     /gene="boxB"
                     /locus_tag="VD788_09695"
     CDS             complement(649..2055)
                     /gene="boxB"
                     /locus_tag="VD788_09695"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: protein motif:HMM:TIGR03225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HXV76581.1"
                     /translation="MQLFDKIPNNVNLSHNRRLQRALEKWQPNYLKWWHEAGPADYQA
                     DEIYLRTAISVDAKGWAHFDYVKMPDYRWGIFLAPPEPDRRVPFGDSYGDPAWQEVPG
                     EFRNALRRIIVTQGDTEPASVEQQRKLSATAPSQYDCRNLFQVNVEEGRHLWAMVYLL
                     HAHFGRDGREEAEELLERRSGDFDKPRILTTFNEPIDDWLSFFMFTMFTDRDGKYQLL
                     ALAESGFDPLARTTRFMLTEEAHHMFVGETGVGRVIKRACELMKQDRNEDARAQGGVD
                     LETVQRYVNFWYSSSLDLFGGEISSNAADYFAAGLKGRAKEERFDDHRALDGTYTIEV
                     LANGGVEPRAVPLRNAMNEILRDAYVDDNQRGVDRWNKTIREEGIDFEIRLPNRRFNR
                     KMGIHAGSWFDPDGNRITPEEFEAKRNGWLPSDADKSYIRQLMVPVLERGKIANWLAP
                     PARGINGQPFDFEYVRRV"
     gene            complement(2055..>2363)
                     /locus_tag="VD788_09700"
     CDS             complement(2055..>2363)
                     /locus_tag="VD788_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76582.1"
                     /translation="EGPIPTEEADELGLVTTAPDEIDWDDDVRIAIEERASLSPDALT
                     GMEASLRFPGGENCDSKIFGRLSAWQNWIFQRPNAVGESGALTLYGRPERPRFDYRRT
                     "
BASE COUNT          364 a          829 c          762 g          408 t
ORIGIN      
        1 gaaggcgaga ccgcacggct tcgcctcgac ccgcacggcc gggaacccgt cgacgacctc
       61 ggcggcatcc cgcgtcagcc ggtcgagcag gcgtcgcgcg ccggcctcga cctccggtgc
      121 cgacccaccg gggtatgcct cgccctcgag gccgtggctg ccgaagcgga gcaacccgtc
      181 gagcgggagc atcctccgca cctggccgag cggccggccc gagaccggcg cgaccgtcca
      241 gccgccgtcg accagacggg cgagggcccg caacgtgccg gccgggacgg tcgcctcctc
      301 cggggtcggt gcgatcggtg ccagtgtacc gtcgacgtcg agcagcagcg ctcgggccga
      361 atcgacggcc gcggctgcgc gtcgcacctg ccgcgcgagg tcgtcgggat cggccaaccg
      421 cacgcttcgc ggcatacgct atccgccgcg ctcaatcgtg cgagcgctca cgacgggaga
      481 ggccagcgcg atgccgaaac cgcgcgcgac cccgtcggaa atccgattgg cccgcacgcg
      541 ccggcgcgat gcgatcgtcc tgtcgcgctg cgcaccttcg tccattgcgg aaaggtgtac
      601 ggcaggcgat ccgacaggac gatcagcgga tcgctgcgac gtcaccgctc aaacccgacg
      661 cacgtactcg aagtcgaacg gctgtccgtt gattccccgc gcgggcggag cgagccagtt
      721 cgcgatcttg cctcgctcga gcaccggcac catcaactgc ctgatgtagc tcttgtccgc
      781 atcgctcggc aaccacccgt tgcgcttggc ctcgaactcc tcgggcgtga tccgattccc
      841 gtccgggtcg aaccacgatc cggcatggat ccccatcttc cggttgaacc gacggttcgg
      901 cagacgaatc tcgaagtcga tcccctcctc gcgaatcgtc ttgttccaac gatcgacgcc
      961 acgctgattg tcgtcgacat acgcgtcgcg caagatctcg ttcatcgcgt tgcgcagcgg
     1021 cacggcgcgc ggctcgacgc ctccgttggc caacacctcg atcgtgtacg tcccgtcgag
     1081 cgctcgatga tcgtcgaagc gctcttcctt ggcgcgaccc ttgaggccgg cggcgaagta
     1141 atcggcggcg ttcgacgaga tctcgccgcc gaacaggtcg agactcgacg agtaccagaa
     1201 gttgacgtat cgctgcaccg tctcgagatc gacaccgccc tgcgcgcggg catcctcgtt
     1261 gcgatcctgc ttcatcagct cgcacgcccg cttgatcacg cgtccgaccc ctgtttcgcc
     1321 gacgaacatg tggtgagctt cctcggtcaa catgaacctc gtcgtgcgcg ccagcgggtc
     1381 gaatccgctc tcggccagtg ccagcagctg gtacttgccg tcacggtcgg tgaacatcgt
     1441 gaacatgaag aacgacagcc aatcgtcgat cggctcgttg aaggtggtga gaatgcgcgg
     1501 cttgtcgaag tcgcccgagc gtcgctcgag cagctcctcg gcctcttcgc gaccgtcgcg
     1561 accgaagtgg gcgtgcaaca ggtagaccat cgcccacagg tggcggccct cctcgacgtt
     1621 gacctggaac agattgcggc agtcgtactg actcggcgcc gtggccgaca gcttccgttg
     1681 ctgttccacg ctcgccggtt ccgtgtcccc ctgggtgacg atgatgcggc gcaacgcgtt
     1741 gcggaactcg cccggcacct cttgccaggc cgggtcgccg taggagtcgc cgaacggcac
     1801 ccgtcggtcg ggctccggcg gcgcgagaaa gatcccccag cggtagtccg gcatcttgac
     1861 gtaatcgaag tgggcccagc ccttggcgtc gaccgagatc gcggtgcgca ggtagatctc
     1921 gtcggcctgg tagtcggcgg ggccggcctc gtgccaccac ttcaggtagt tcggctgcca
     1981 tttctcgagc gcgcgctgca accgccggtt gtgtgacagg ttgacgttgt tggggatctt
     2041 gtcgaacagc tgcatcaggt tctcctgtag tcgaatcgcg gtcgctccgg ccggccgtac
     2101 aacgtgagcg cgccgctctc gccgaccgcg ttgggccgct ggaagatcca gttctgccag
     2161 gccgacaagc ggccgaagat cttgctatcg cagttctcgc caccggggaa tcgcaggctg
     2221 gcctccatcc cggtcagcgc gtcgggcgac aggctcgccc gctcctcgat cgcgatccgg
     2281 acgtcgtcgt cccagtcgat ctcgtcgggg gcggtggtga cgaggccgag ctcgtcggcc
     2341 tcctcggtcg ggatcggccc ctc
//
LOCUS       DASPGN010000337         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1699835, whole genome shotgun sequence.
ACCESSION   DASPGN010000337 DASPGN010000000
VERSION     DASPGN010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699835"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /gene="dut"
                     /locus_tag="VD788_09705"
     CDS             complement(<1..341)
                     /gene="dut"
                     /locus_tag="VD788_09705"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HXV76583.1"
                     /translation="MKPTPAVERPLAEVRVRRLPGCEDLPLPTAATEHATGLDLRAAV
                     TAAVRLAPGQRLLIPTGIAIALPGGFEAQIRPRSGLALRHGVTLVNSPGTIDADYRGE
                     IGVVLINHGERT"
     gene            complement(338..1600)
                     /locus_tag="VD788_09710"
     CDS             complement(338..1600)
                     /locus_tag="VD788_09710"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXV76584.1"
                     /translation="MIRKTTFENGLTLLTESMPDVRSVCLGVWLRRGSRDEPAAINGI
                     SHFIEHLVFKGTDSRSARDIALEMDAIGGQMDAFTSKEYTCFYAKVLDEHLDGAVELL
                     ADVVQNPAFDPRELERERLVVLEEIRMVEDTPDELLFDLFSERVYPAHPLGRPIQGTE
                     KTVSAMSRRRLLGFFHQAYCPSNMLIVAAGSLRHARLSRLVGQAFAGLRRNGRRKPGS
                     AKPVFRSALALRDKPELEQLHLLLGLPAFPTTLAQRYALHVLNTILGGTMSSRLFQRI
                     REERGLAYSVYSALNLFSDSGVLALYAGTSPPRGDEAIVVIRDELRALADAGPTEDEI
                     HVAREHLKGSLMLSLESTSSRMSNLARQEIYFGRELGLAETLAGIERVDAGQIKRMAR
                     RLFRGRRIGLAAVGRVGALRVRARDLVV"
     gene            complement(1691..>2097)
                     /locus_tag="VD788_09715"
     CDS             complement(1691..>2097)
                     /locus_tag="VD788_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76585.1"
                     /translation="DDAAEAQTRSPLAGGRERQAGLPGQRGDAAGPAPQLKPISSDDP
                     GADPNGGGKQYGGLPIAGVRTLSDEEPFRVYNGQNEYAQWRFTYIDYELQAAGRGAQP
                     GPPSPPGPGTREQTAPRSKAPGRLPTGDRSDR"
BASE COUNT          292 a          732 c          757 g          316 t
ORIGIN      
        1 gtgcgctcgc cgtggttgat cagcacgacg ccgatctcgc cgcggtagtc ggcatcgatc
       61 gttcccgggc tgttgaccag cgtcacgccg tgccgcagag ccagaccgct cctcggccgg
      121 atctgcgctt cgaagccgcc gggcagcgcg atcgcgatcc cggtcgggat caacaggcgc
      181 tgtcccggcg ccagtcggac cgcggccgtc accgccgcgc gcagatcgag gcccgtcgcg
      241 tgctcggtgg cggccgtcgg cagcggcagg tcctcgcagc ccggcaagcg acgcacgcgg
      301 acctcggcca gcggacgctc gactgccggg gtcggcttca aaccacgagg tcccgcgcgc
      361 gcacgcgcaa cgcgccgacg cggccgaccg cggccagccc gatgcgacgg ccgcggaaca
      421 gccggcgcgc catccgtttg atctggccgg cgtccacacg ctcgatgccg gcgagcgtct
      481 cggccaagcc gagctcgcgg ccgaagtaga tctcctggcg ggcgaggttg gacatccggc
      541 tgctcgtcga ctcgagcgac agcatcaggc tgcccttgag atgctcgcgc gcgacgtgga
      601 tctcgtcctc ggtcggtccg gcgtcggcca gcgcgcgcag ctcgtcgcgg atgaccacga
      661 tcgcctcgtc gccgcgcggt gggctcgtgc cggcgtacag cgcgagtacg cccgaatcgg
      721 agaacagatt gagcgccgag tagacgctgt aggccagacc tcgctcttcg cgaatccgtt
      781 gaaacagtcg cgaggacatc gtgccgccga gaatcgtgtt caacacgtgg agcgcgtagc
      841 gctgggcgag cgtcgtcggg aaggccggta gaccgagcag caggtggagc tgttcgagct
      901 ccggtttgtc gcgcagcgcg agcgccgagc gaaagaccgg cttggccgac cccggcttgc
      961 gccggccgtt ccggcgcagc ccggcgaacg cctggcccac gagtcgcgaa agacgggcgt
     1021 gcctcaagct gccggcggcg acgatcaaca tgttcgacgg gcagtaggcc tggtggaaga
     1081 agccgagcag ccgccgccgg ctcatcgcgc tgaccgtctt ctcggtcccc tggatcggcc
     1141 gcccgagcgg atgggccggg tagacgcgct cggaaaaaag gtcgaacagc agctcgtccg
     1201 gcgtgtcctc gaccatgcgg atctcttcca gcacgaccag ccgctcgcgc tcgagctcgc
     1261 gcgggtcgaa cgccgggttc tgcacgacgt cggccagcag ctcgacggcc ccgtcgaggt
     1321 gctcgtcgag caccttggcg tagaagcagg tgtactcctt cgaggtgaag gcgtccatct
     1381 gcccgccgat ggcgtccatc tcgagcgcga tgtcgcgggc gctgcggctg tcggtgccct
     1441 tgaacacgag gtgctcgatg aaatgcgaga tgccgttgat tgccgccggc tcgtcacggc
     1501 tgccgcgccg cagccagacg ccgaggcaga ccgaccgcac gtcgggcatc gactcggtca
     1561 gcagggtcag accgttctcg aacgtcgttt tacggatcac ggcgggggta cccctggacg
     1621 tcgccggctg cagcccgagg cggccgcagg ccgggggcgc gggcgctcgg acgcggagta
     1681 taccacgcgg tcagcggtcc gatcggtccc cggtcggcag ccggcccggg gctttggagc
     1741 ggggcgctgt ttgctccctc gttccggggc ccggtgggct cggtgggccc ggttgcgcac
     1801 cgcgacccgc ggcctgcagc tcgtagtcga tataggtgaa gcgccactgg gcgtactcgt
     1861 tctggccgtt gtagacgcgg aacggttcct cgtccgacag cgttcggacg ccggcgatcg
     1921 gcaacccgcc gtactgcttg ccgccgccgt tgggatcggc gccgggatcg tccgaggaga
     1981 tcggcttgag ctgcggcgcc ggcccggcgg cgtcgcctcg ctggccgggt aggccggcct
     2041 gccgctcgcg gccgcccgcg agcggacttc gcgtttgcgc ctccgccgcg tcgtctc
//
LOCUS       DASPGN010000338         7442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_996400, whole genome shotgun sequence.
ACCESSION   DASPGN010000338 DASPGN010000000
VERSION     DASPGN010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996400"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VD788_09720"
     CDS             <1..590
                     /locus_tag="VD788_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76586.1"
                     /translation="FLTTRAGIRRRHRSNGHARSRYHQRPISRMGVLTVEADGGLPAP
                     RLKQVLLDEIRRSRSPFGIRIIEATSGETATDAYNFQAFLGEINLAAKVYPDGREQWI
                     RGVNFVGTPLNGIRGIVAAGDRSAVDNAFCGAESGYVPVSTISPALVLSELELQSKPD
                     SEYAQFSLPLPWRSDRRGRRRAPAGRNRPDRYTPG"
     gene            592..2370
                     /locus_tag="VD788_09725"
     CDS             592..2370
                     /locus_tag="VD788_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV76587.1"
                     /translation="MASGRLVRLARPHWPAFALGFALLLATNGLALSLPWLLREAIHA
                     MERGAALGVIARLALAMIVVALVQAVVRTASRLSILGASRKIACEVRDTFFSQLTHMD
                     AGYYDTHRTGDIMSRGVNDIQLIQSFFGPGVMNLLNTTIVYGATLVLLLGISVELTVV
                     SLMLFPVLFFVVNRLSDKVYAYSKAVQEQLSTISNRSQENISGIQQVKTYVQEEREIA
                     AFRELCEEFRRRNLSMATVRGAMVALIGVVTGIGTLIVLFVGGRFVIEGRIGFGDFVA
                     FNAYLAMLSWPTVALGWIINTFQRGVGAMERLDEVFDSRPAIPPAAEASAASSAGTTA
                     PVEGDIEIRRLTFAYDGAAPPDGPAAPAALCDVSITIEAGSRVALVGPVGSGKSTLAG
                     LIARVYPAPRGTIFIGGSDINDIPVERLRASIGYVPQEAFLFSRSLRDNVAFGQPDAT
                     EEALSRALEVSHLGRDVAAFPSGADTIVGERGVTLSGGQRQRATLARAVIGEPRILIL
                     DDALSSVDADTERAILDQLDRLMRGRTSILISHRFSSLAGMDRIVVLDRGRVVEQGTH
                     GELLERGGLYARLYRRHRLETTLEQR"
     gene            2367..4154
                     /locus_tag="VD788_09730"
     CDS             2367..4154
                     /locus_tag="VD788_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV76588.1"
                     /translation="MSAPSSGIHEEAALGKAYDARLIRRPWRYVRPHRGLVLASLVTM
                     LAVSAAQLVQPYLIKIAIDQHIAAGRLAGLGTVAGLFLLALLGEFALRFAQLYVLQKT
                     GQNVVLDLRMELFSHLQHLPSAFFDRNPVGRLMTRLTTDVEALNEAFTSGLVVILADL
                     IKLVGIVAILLWMDWRLALVTFAILPPTLVLTRLFRDRMRAAYREVRVWVARVNAFLQ
                     ENVSGMRVVQLFSREAAMRRQFGEINRAHRDSQLRGVRYDSIFSALAELIGSVTLAAI
                     VWGGGWGVLRGIVTFGTLVAFIDYAGKFFGPVQELSQRYTVMQSAMAAAERIFALLDT
                     KAAVRSPSRPYAAPGRLRGEIVFERVNFGYVEGEPVLHDVSFTIRPGQRIAVVGWTGS
                     GKSTLIRLLIRLYDVWDGRILLDGVDVRDYDLQDLRRRVGIVLQDHFLFAGNVEGNIS
                     LGDPRVTPRRVREAAAAVHADRFIERLPRGYDEPVRERGSNFSVGQKQLLSFARAIAF
                     DPAILVLDEATASVDPETEHHIREALATLLDGRTSIVIAHRLATLRDVDRILVLHHGR
                     LVEQGTHDELVRLDAGIYRTLYTLQAATG"
     gene            4220..7273
                     /locus_tag="VD788_09735"
     CDS             4220..7273
                     /locus_tag="VD788_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76589.1"
                     /translation="MPTVPRVPRSALAVLCVAAAISTRAEYVNFESAHVHALDLTPQR
                     TRLLAVNTPGATLEIFAVASDGGLTPLATVPVGLEPVGVVARTDSEAWVVNQLSDTVS
                     VVDLDGAVVVRTLSVGDEPTDVAFAQGRAFVSLAGVDALEVFDLTALTAPPVRVELFG
                     RKPRAMAVSSDGARLYVVPLMSGNRTTVVHAGVILANDANLDPGRLAALGLEDLTCDG
                     APPAYPSLPDGITRRPDLPPLPGGVAPVGLIVSWNEATDRWQDETGADWSHCLPFRLP
                     DHDLFVVSAGDLSVTTVDRLGTSLFDVSVNPGNGKIYIPHTDARNFVRFEHPQGVQGH
                     VVDNRIAIVDPSDGYSVTLVDLNDHIDRGSDPATNLAERRASISQPGMMAWSADGTRA
                     WLVGLGSRKVFRLDAACETGACIFGADRADPAVVEVGEGPSAVALNEPADRLYVLNRF
                     SNSIAIVDTEALAKVSEIELHDPTPLATLDGRRFLYDAIDGSGHGDNACSSCHLFGDR
                     DDLAWDLGNPGGDSVPYTTIDDNVRFVIPVGGEPLTCDPALCAAQTGFDPQKGPMTTQ
                     TLRGMLEPLHWRGDRATMNNFNQAFPGLMGTADIGPINGEPAGLSTEEMERFRRFALA
                     IRYPPNPNRNIDDTLPDADLRLPGSGVDGNPLAGELVFNEQPTDAGQPCVSCHSHPFG
                     AAGGTVGGVEPAEPTSLATAALFNGNADQSFHSDVKVAHLRNMYEKRGPTFGDHVGAD
                     PPAKSGVGFGHDGSIPDLLTFFSFNVFTMTAEQVADVAAFSLHFPTGTRPAVGRQLTV
                     PAGSPPTAPPAAESLLATLIGLGDLAGASRHCELVASAEHGGRVRAFHLSGGLWVTDV
                     AAEAPLATGALRQQATSPVTFLCVTPGSGPRLGGDRDQDVVPDGDDCAASDPETWRGP
                     AESAGVTLARGGATTLVWNEQATSSGPSVRYDVLGGSLASLRADGGVGGASCVAGALV
                     APDYADTRPDPAAGEGLYYLVRGVNDCAVGTAGAGREPLDARICP"
BASE COUNT         1036 a         2592 c         2608 g         1206 t
ORIGIN      
        1 acttcctcac gacgcgcgcg ggtatccgcc ggcgccaccg gtcgaacggc cacgcccgca
       61 gccgctacca ccagcggccg atcagccgga tgggcgtgtt gaccgtcgag gccgacggcg
      121 gtttgcccgc cccgcggttg aagcaggtac tgctcgacga gatccggcgc agcagatcgc
      181 cgttcggcat ccgcatcatc gaggcgacga gcggcgagac cgcgaccgac gcctacaact
      241 tccaggcgtt tctcggcgag atcaacctcg ccgccaaggt ctatcccgac ggccgcgagc
      301 agtggattcg cggcgtcaac ttcgtcggca ctccgctcaa cgggatccgc gggatcgtcg
      361 ccgcgggcga tcgctcggcg gtcgacaacg cgttctgcgg agccgagtcg ggctacgtcc
      421 cggtgtcgac gatcagcccc gcgctggtgc tgtccgagct cgagctgcag agcaagccgg
      481 actcggagta cgcgcagttc agcctgccgc tgccgtggcg gtccgatcgc cgggggcggc
      541 gtcgcgcgcc ggctggacga aatcgtccgg accgctacac tcccggctga gatggcttcc
      601 ggtcgtttgg tccgtctggc gcgcccgcac tggccggcgt tcgcgctcgg tttcgccctg
      661 ctgttggcga ccaacggcct ggcgctgtcg ttgccgtggt tgctgcgcga ggccattcac
      721 gcgatggagc gcggcgcggc gctcggcgtg atcgcgcggc tcgcgctggc gatgatcgtc
      781 gtggcgttgg tccaggcggt ggtgcgcacc gcctcacggc tgtcgatcct gggcgccagc
      841 cgcaagatcg cctgcgaggt gcgtgatacg ttcttctcgc agctcacgca catggacgcc
      901 ggctactacg acacgcaccg caccggcgac atcatgtccc gcggcgtcaa cgacatccag
      961 ctgatccagt cgttcttcgg gcccggagtg atgaacctgt tgaacacgac gatcgtctac
     1021 ggcgcgacgc tggtgctgct gctcggcatc agcgtcgagc tgacggtggt ctcgttgatg
     1081 ctgttcccgg tgctgttctt cgtcgtcaac cgcttgagcg acaaggtcta cgcctactcc
     1141 aaggcggtgc aggagcagct ctccacgatc tcgaaccgct cgcaggagaa catcagcggt
     1201 atccagcagg tcaagaccta cgtccaggaa gagcgcgaga tcgccgcttt tcgcgagctg
     1261 tgcgaagagt tccgtcggcg caatctgtcg atggcgacgg tgcgcggcgc gatggtggcg
     1321 ttgatcggcg tggtcaccgg gatcggcacg ttgatcgtgc tgttcgtcgg cgggcggttc
     1381 gtgatcgagg ggcggatcgg cttcggcgac ttcgtcgcgt tcaacgccta cctggcgatg
     1441 ctgagttggc cgacggtggc gctgggctgg atcatcaaca ccttccagcg cggcgtcgga
     1501 gcgatggagc gcctggacga ggtgttcgac agcaggcccg cgatcccgcc ggcggccgaa
     1561 gcctccgccg cgtcgtcggc cggcacgacc gcgccggtcg agggcgacat cgagatccgg
     1621 cgactgacct tcgcctacga cggagcggcg ccgcccgacg ggccggccgc cccggcggcc
     1681 ctgtgcgacg tctcgatcac gatcgaggcc ggcagccggg tcgcgcttgt cggcccggtc
     1741 ggttcgggca agtcgacgct ggccggtctg atcgcacgtg tctatcccgc tccgcgcggg
     1801 acgatcttca tcggcggcag cgacatcaac gacattccgg tcgagcggct gcgcgccagc
     1861 atcggctacg tgccgcagga ggccttcctg ttctcgcggt cgctgcgcga caacgtcgcc
     1921 ttcggccagc cggacgccac cgaggaagcg ttgtcgcgcg cgttggaggt gtcgcacctc
     1981 gggcgagacg tcgccgcctt cccctcgggg gccgacacga tcgtcggcga gcgcggcgtc
     2041 acgctgtcgg gcggtcagcg ccagcgcgcc acgctggccc gcgcagtgat cggcgagccg
     2101 aggatcctga tcctggatga cgcgctgtcg agcgtcgacg ccgacaccga gcgggcgata
     2161 ctcgaccagc tcgaccggct gatgcgcggc aggacgtcga tcctgatctc gcaccgattc
     2221 tcgtcgctgg ccgggatgga ccgcatcgtc gtgctcgacc ggggacgggt cgtcgagcag
     2281 gggacccacg gcgagctgct cgaacggggc ggcctctacg cgcggctgta ccggcgccat
     2341 cggctggaaa ccacgctgga gcagcgatga gcgcgccgtc atccggaatc cacgaggagg
     2401 ccgcgctggg caaggcctac gacgcgcggc tgatccgtcg gccgtggcgt tacgtccggc
     2461 cgcaccgcgg gttggtgctg gcatcgctcg tgacgatgct ggcggtcagc gcggcgcagc
     2521 tggtccagcc ctacctgatc aagatcgcga tcgaccagca catcgcggcc ggccggctgg
     2581 cggggctggg gacggtcgcc ggattgttcc tgctggccct gttgggcgag ttcgcgctgc
     2641 gcttcgcgca gctctacgtg ctgcagaaga ccggccagaa cgtcgtgctc gatctgcgga
     2701 tggagctgtt ttcgcacctg cagcacctgc cgtccgcgtt cttcgaccgc aacccggtcg
     2761 gacgattgat gacgcggttg acgaccgacg tcgaggcgct caacgaggcg tttacctcgg
     2821 gcctggtcgt catcctggcc gatctgatca agctcgtcgg catcgtcgcg atcctgttgt
     2881 ggatggactg gcgtctggcg ctcgtgacct tcgcgatctt gccgccgacg ctggtgctga
     2941 cccggctgtt ccgcgaccgt atgcgagcgg cctaccgcga ggtgcgcgtc tgggtcgccc
     3001 gcgtcaacgc cttcctccag gaaaacgtct cgggcatgcg ggtcgtccag ctgttctcgc
     3061 gcgaggcggc gatgcgccga cagttcggtg agatcaaccg tgcgcaccgc gactcgcagc
     3121 tgcgcggggt tcgctatgat tcgatcttct ccgcgctggc cgagctgatc gggtcggtca
     3181 cgctggccgc gatcgtctgg ggcggcggct ggggcgttct gcgcggcatc gtcaccttcg
     3241 gcacgttggt cgcgttcatc gactacgccg gcaagttctt cggaccggtg caggagctgt
     3301 cccagcgcta cacggtgatg caatcggcga tggccgccgc cgaacggatc ttcgcgttgc
     3361 tcgacaccaa ggccgcggtg cgctcgccgt cgcgaccgta cgccgctccc gggcggttgc
     3421 gcggcgagat cgtcttcgag cgcgtcaact tcggctacgt cgaaggcgaa ccggtactgc
     3481 atgacgtctc gttcaccatc cggcccggcc agcgcatcgc cgtcgtcggc tggaccgggt
     3541 cgggcaagtc gacgctgatc cgcctgttga tccggctcta cgacgtgtgg gacggccgca
     3601 tcctgctcga cggcgtcgac gtgcgcgact acgacctgca ggacttgcgc cggcgggtcg
     3661 gcatcgtgct gcaggatcac ttcctgttcg ccgggaacgt cgagggcaac atctcgctgg
     3721 gcgatccgcg cgtgacgccg cggagggtgc gggaggcggc tgcggcggtc cacgccgacc
     3781 gcttcatcga gcggttgccg cgaggctacg acgagccggt gcgggaacgc ggctcgaatt
     3841 tctcggtcgg gcagaagcag ctgctgtcgt tcgcgcgggc gatcgcgttc gacccggcga
     3901 tcctggtgct cgacgaggcg acggcctcgg tcgaccccga gaccgagcat cacattcgcg
     3961 aggcgctggc cacgttgctc gacggtcgca cctcgatcgt catcgcgcac cggctggcca
     4021 cgctgcggga cgtcgaccgg atcctggtgc tgcaccacgg gcggctggtc gagcaaggca
     4081 cgcacgacga gctggtccgg ctcgacgcgg ggatctaccg cacgctctac acgctgcagg
     4141 ccgcgaccgg ctgatcgccg gatcacgcgg cgccgcgccc ggcttgccgc ggcgcgcacc
     4201 atgccgtata gagtcgggca tgccaaccgt cccccgggtt ccgcgctcgg cgctcgccgt
     4261 gttgtgcgtc gcggcggcga tctcgacgcg ggctgagtac gtcaacttcg agtcggccca
     4321 cgtgcacgcg ctcgatctga cgccgcagcg cacccgcctg ttggccgtga acacgcccgg
     4381 cgcgacgctc gagatcttcg ccgtggcgag cgacggcggg ctgacgccgc tggcgacggt
     4441 ccccgtcggg ctcgagcccg tcggtgtcgt cgcccgtacc gacagcgagg cgtgggtcgt
     4501 caaccagctg tccgacacgg tcagcgtcgt cgatctcgac ggcgccgtcg tcgtgcgcac
     4561 gctgtcggtc ggcgacgagc cgaccgacgt cgccttcgcc caggggcggg ccttcgtctc
     4621 gctggccggc gtcgacgcgc tcgaggtgtt cgacctgacc gcgctgaccg cgccgccggt
     4681 gcgagtcgag ctgttcgggc gcaagccgcg cgcgatggcg gtctcgagcg acggcgcccg
     4741 gctgtacgtg gtcccgctga tgtcgggcaa ccggacgacc gtggtccatg ccggcgtgat
     4801 cttggccaac gacgcgaacc tcgatcccgg ccggctggcc gcgctgggtc tcgaggatct
     4861 gacctgtgac ggcgcgccgc cggcctaccc gtcgctgccg gacggcatca cgcggcggcc
     4921 cgacctgccg ccgctgcccg gcggcgtcgc accggtcggg ctgatcgtga gttggaacga
     4981 ggcgaccgat cgctggcagg acgagaccgg cgccgactgg tcgcattgcc tgccgttcag
     5041 gctgcccgac cacgacctgt tcgtcgtctc cgccggcgat ctgtcggtga ccacggtcga
     5101 tcgtctcgga acgtcgctgt tcgacgtgtc ggtcaatccc ggcaacggca agatctacat
     5161 accccacacc gacgcccgca acttcgtccg cttcgagcac ccgcaggggg ttcagggcca
     5221 cgtcgtcgac aaccgcatcg cgatcgtcga cccgagcgac ggctactcgg tgacgctcgt
     5281 cgacctcaac gatcacatcg accgcggcag cgacccggcg acgaacctcg ccgagcggcg
     5341 ggccagcatc tcccagcccg gcatgatggc gtggagcgcg gacggtacgc gggcgtggct
     5401 cgtcggcctc ggctcgcgca aggtctttcg cctcgacgcg gcgtgcgaga ccggcgcgtg
     5461 cattttcggg gccgaccgag ccgacccggc cgtcgtcgag gtcggcgagg ggccttctgc
     5521 ggtggcgctc aacgagccgg ccgaccggct ctacgtgctg aaccgcttct ccaactcgat
     5581 cgcgatcgtc gacaccgagg cgttggccaa ggtcagcgag atcgagctgc acgacccgac
     5641 gccgcttgcc acgctcgacg gacggcgctt cctctacgac gcgatcgacg gctcgggtca
     5701 cggcgacaac gcctgctcga gctgccatct gttcggcgat cgcgacgatc tggcgtggga
     5761 cctcggcaac cccgggggag attccgtccc gtacacgacg atcgacgaca acgtgcgttt
     5821 cgtcatcccg gtcggcggcg agccgttgac ctgcgatccc gcgttgtgcg cggcacagac
     5881 cggcttcgat ccgcaaaaag gaccgatgac gacgcagacc ttgcgcggga tgctcgagcc
     5941 gttgcactgg cgtggtgatc gggcgacgat gaacaacttc aaccaagcgt tccccggtct
     6001 gatgggtacg gccgacatcg gcccgatcaa cggcgagccg gccgggctga gcacggagga
     6061 gatggagcgg ttccgccgat tcgcgctggc catccgctat ccgcccaacc cgaatcgcaa
     6121 catcgacgac acgctgcccg acgccgacct gcggctgccc gggagcggcg tcgacggcaa
     6181 cccgctggcc ggtgagctcg tcttcaacga gcagccgacc gatgccggcc agccgtgcgt
     6241 gtcgtgccac agccatccgt tcggcgcggc cggcgggact gtcggcggcg tcgagcccgc
     6301 cgagccgacc tcgctcgcca ccgccgcgct gttcaacggc aacgcggacc agtcgttcca
     6361 cagcgacgtc aaggtggccc atctacgcaa catgtacgaa aagcgaggcc cgaccttcgg
     6421 cgaccacgtc ggcgccgacc cgccggccaa gagcggcgtc ggcttcggcc acgacgggtc
     6481 gattcccgac ctgttgacgt ttttctcgtt caacgtcttc acgatgaccg ccgagcaagt
     6541 ggcagacgtc gcggcgttca gcctgcactt cccgaccggc acccggccgg cagtcgggcg
     6601 gcagctcacc gtgccggcag gatcaccgcc gacggcgccg cccgcggccg aatcgttgct
     6661 cgcgaccttg atcgggctgg gagacctggc cggcgcctcc cgccactgcg agctcgtcgc
     6721 gtcggccgag cacggcggtc gcgtgcgggc gttccacctg tcgggcggcc tctgggtgac
     6781 cgacgtcgcc gccgaggcgc cgctcgcgac cggcgccctg cgccagcagg cgacctcgcc
     6841 cgtcacgttc ctgtgcgtca cacccggctc gggaccgagg ttgggcgggg accgcgatca
     6901 ggacgtcgtg cccgacggcg acgactgcgc cgcctccgat cccgagacct ggcgcggacc
     6961 ggccgagagc gccggcgtga ccctcgcccg cggtggtgcg acgacgctcg tctggaacga
     7021 gcaggcgacg tcgagcggcc cgtcggtgcg ctacgacgtg ctgggcggca gtctcgcgtc
     7081 gctgcgcgcg gatggcgggg tcggcggcgc gagctgcgtc gccggcgcgc tcgtcgcgcc
     7141 ggactacgcc gacacgcgcc ccgatccggc cgcgggggag ggcctctact atctcgtccg
     7201 cggcgtcaac gactgcgcgg tgggcaccgc cggtgccggc cgcgagccgc tcgacgcgcg
     7261 gatttgcccg tgaagtgatc ggcctcgtta gagacaggac actaggagcc tgtccgagta
     7321 accggggctg attgagttcg gctggcggta gcgagcatga tcatccaatc gtgcagtcga
     7381 gatcggcggc gccgcgggcg ccgaacgact ccgtgctgag gttggcccct gtcgctgggg
     7441 tg
//
LOCUS       DASPGN010000339         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_296214, whole genome shotgun sequence.
ACCESSION   DASPGN010000339 DASPGN010000000
VERSION     DASPGN010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296214"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1291)
                     /locus_tag="VD788_09740"
     CDS             complement(83..1291)
                     /locus_tag="VD788_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV76590.1"
                     /translation="MGRTKRHGAPGGGRRVVTRSIYFGDEHEAFRDEVRRFLVHEVAP
                     HAERWERDGRIPREIFGRMGRLGYLGITVPERYGGSGADLLYAAVFLEELPRSMMGGF
                     NAAVSVQQFMATPHILRAGSDALIRRFVVPSVEGRAVGALAITEPDAGSDVAAIRTTA
                     RRDGDAFVVNGAKTFITNGADGDFLTLAVRTGDAGAGGISLLAVERDTPGVRVANRLA
                     KLGWHASDTAELVFEDARVPADRLIGRENGGFAQLMESFALERLCSALIAVGGAHVTL
                     ELTLEHIARRRAFGSRIDRFQAIRHRVADMVAEIEAARQLSYHVAWLLDRGETATREA
                     AMAKLVATELAKRVADECLQFFGGYGFMEEYPIARLWRDARGGTITAGTSEIMREIVA
                     RETIDRSDED"
     gene            complement(1240..1626)
                     /locus_tag="VD788_09745"
     CDS             complement(1240..1626)
                     /locus_tag="VD788_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76591.1"
                     /translation="MKARYLCLAAALLSAVGCSAPVAHYRCDFDARGVEPDVTTVWKC
                     NRDVIERVVDGERFSLREFRGAARFFSESTGIPADTRETRQGTQPGLGLRENLDQWDA
                     WYVAHRDSLVWDERSGTVRPAAGGAS"
     gene            complement(1659..>2994)
                     /locus_tag="VD788_09750"
     CDS             complement(1659..>2994)
                     /locus_tag="VD788_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HXV76592.1"
                     /translation="VGLARESELFGKLTAGPVAKQLIGLFDGMNELKKPLPGAAPRPV
                     RRLAVLGGGFMGSGIATVSVPLVPVTVKDVSDDVLARCARSVLEGLDQRVRSGAITTF
                     ERDRQLAGLHLTRNDGELARADLIVEAVFEDLALKREILATTEQVVSDEAVFASNTSA
                     LPITEIAAGARRPERVLGMHYFSPVPKMPLLELVVTAQTSDGAVATARDFAIRQGKTV
                     IVVRDGPGFYTSRILSPYLGEALRLVEEGARVEAIDGALRDFGFPVGPMALLDEVGID
                     VVAHVARNFGERYARRGLHGGEGFARLHEAGYAGRKNKRGFYRYGDSGGKKRKKTVNE
                     EVYAVTGGAPRKAFAAGEIADRLALLMINEAIYCLDEGVIASPRDGDIGAILGLGFPP
                     FCGGPFRYVDHCGPRELIERMERLRDRCGPRFEPAPALRALARDGSGFRVGS"
BASE COUNT          460 a         1105 c         1008 g          421 t
ORIGIN      
        1 gaagtcgacc ggcgctcgac ccgccgcctt gtccgggagc gagcgcatca tctgttcgac
       61 ggtcaccacc tggcctccct cgtcagtcct cgtccgagcg gtcgatcgtc tctcgcgcga
      121 cgatctcgcg catgatctcg gaggttcccg cggtgatcgt cccgccgcgg gcgtcgcgcc
      181 acagccgcgc gatcgggtat tcctccatga agccgtagcc accgaagaac tgcaggcact
      241 cgtcggcgac gcgcttggcc agctcggtcg ctacgagctt ggccatcgcg gcctcgcgcg
      301 tcgcggtctc gccccggtcg agcagccagg ccacgtgata ggacagctgc cgcgccgctt
      361 cgatctcggc caccatgtcg gcgacgcggt gacggatcgc ctggaaacgg tcgatccgcg
      421 agccgaacgc ccggcgtcga gcgatgtgct cgagcgtcag ctcgagcgtc acgtgggccc
      481 cgccgacggc gatcagcgcg gagcacagcc gctcgagcgc gaacgattcc atcaattgcg
      541 cgaacccgcc gttctcgcgg ccgatcagcc ggtccgccgg aacgcgggca tcctcgaaca
      601 ccagctcggc cgtgtcggag gcgtgccaac ccagcttggc caggcggttg gcgacgcgca
      661 caccgggcgt gtcgcgttcg accgcgagca ggctgatccc gccggcaccg gcatcaccgg
      721 tgcgcacggc gagcgtgaga aagtcgccgt cggcgccgtt ggtgatgaag gtcttggcgc
      781 cgttgacgac gaacgcatcg ccgtcgcgac gggccgtggt gcggatcgcg gccacgtccg
      841 agccggcgtc gggctcggtg atcgccagcg caccgaccgc gcgtccctcg accgacggca
      901 cgacgaagcg ccggatcaac gcgtcggagc ccgcgcgcag gatgtgcggc gtggccatga
      961 actgctgcac cgagaccgcg gcgttgaacc cgcccatcat cgatcgcggc agctcctcga
     1021 gaaagaccgc ggcgtacagc aggtccgccc cgctgccgcc gtaccgctcc gggaccgtga
     1081 tgccgagata gccgagacgc cccatccgcc cgaagatctc gcgcgggatg cggccgtccc
     1141 gctcccagcg ttcggcgtgc ggggcgactt cgtggaccag gaagcggcgc acctcgtcgc
     1201 gaaacgcttc gtgctcgtcg ccgaagtaga tcgaccgcgt cacgacgcgc cgcccgccgc
     1261 cgggcgcacc gtgccgcttc gttcgtccca caccagcgaa tcgcggtggg cgacgtacca
     1321 ggcatcccac tggtcgaggt tctcgcgcag accgagcccg ggctgggtgc cctgacgcgt
     1381 ttcgcgcgtg tcggccggga tcccggtcga ttcgctgaag aaccgcgccg cgccgcggaa
     1441 ctcgcgcagg ctgaaccgct cgccgtcgac gacgcgctcg atcacgtcgc ggttgcactt
     1501 ccagacggtg gtgacgtcgg gctcgactcc gcgggcgtcg aaatcgcagc ggtagtgggc
     1561 caccggggcc gaacaaccga ccgccgacag cagggcggcg gcgaggcaca ggtaccgggc
     1621 tttcatcgcc gccacctaac cacgcacgcg ggtcgaattc aagacccgac gcggaagccc
     1681 gatccgtccc gcgccagggc gcgtaaggcg ggcgccggtt cgaagcgcgg cccgcaccgg
     1741 tcgcgcagcc gctccatccg ttcgatcagc tcgcgtggcc cgcagtggtc gacgtagcgg
     1801 aacggtccgc cgcaaaacgg cgggaagccg agtccgagga tcgcgccgat gtcgccgtcg
     1861 cgcggcgagg cgatcacgcc ctcgtcgaga cagtagatcg cctcgttgat catcaacagc
     1921 gccagccggt cggcgatctc ccccgcggcg aacgccttgc gcggcgcgcc gccggtcacc
     1981 gcgtagacct cctcgttgac cgtcttcttt ctcttcttcc ccccgctgtc gccgtagcgg
     2041 tagaaaccac gcttgttctt gcgccccgcg tagccggcct cgtgcagccg agcgaacccc
     2101 tcgccgccgt gcagaccgcg ccgcgcgtag cgctcaccga agttgcgcgc gacgtgcgcc
     2161 accacgtcga tcccgacctc gtcgagcaac gccatcggcc cgaccgggaa accgaagtcg
     2221 cgcagcgcgc cgtcgatcgc ttcgacgcgc gctccctcct cgacgagccg cagcgcctcg
     2281 ccgagatagg gcgacaggat gcgcgaggtg tagaagccgg ggccgtcgcg caccacgatc
     2341 acggtcttgc cctggcggat ggcaaaatca cgcgcggtgg cgacggcccc gtcgctcgtc
     2401 tgcgcggtca ccaccagctc gagcaacggc attttgggaa ccggcgagaa atagtgcatg
     2461 cccagcaccc gctccggacg tcgcgcgccg gccgcgatct cggtgatcgg aagggccgag
     2521 gtgttcgagg cgaagacggc ctcgtccgag acgacctgct cggtcgtggc caggatctcg
     2581 cgtttcagcg cgagatcctc gaacacggcc tcgacgatca ggtcggcgcg ggccagctcg
     2641 ccgtcgttcc gggtcaggtg gagcccggcc agctgccggt cgcgctcgaa cgtcgtgatc
     2701 gcgcccgaac gcacgcgttg gtcgaggcct tcgagcaccg atcgcgcgca gcgggcgagc
     2761 acgtcgtccg aaacgtcctt caccgtcacc ggcacgagcg gcaccgagac ggtggcgatg
     2821 ccgctgccca tgaacccgcc gccgagcacg gcgagccggc ggaccggccg cggcgcggcg
     2881 ccgggcaacg gcttcttcag ctcgttcatc ccgtcgaaca ggccgatcaa ctgcttggcc
     2941 accgggccgg ccgtcaactt gccgaacagc tccgattcgc gggccagacc gacg
//
LOCUS       DASPGN010000340         8748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1700219, whole genome shotgun sequence.
ACCESSION   DASPGN010000340 DASPGN010000000
VERSION     DASPGN010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1700219"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VD788_09755"
     CDS             <1..913
                     /locus_tag="VD788_09755"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HXV76593.1"
                     /translation="VRIDRPVFLVGVQGGGGTIVARTLYRHPRAVYASGNSRFWAGLD
                     EIHNSRHTIRDLPESLIHRSAHFFNVDGRLDCHPIYGYQRCWLYAIDEFLDRYRRTAA
                     DADESTSLALRRVIGKTLLAYAHDLRTARFVDMSQLYTIQLSYIAELLRGCDPRFVLL
                     TRNPYVTVARAARKEYDGHGTKVEMTTEQKMRCAIEHWHNSYRLALDDARGRPLLQVK
                     YEDFLEDPLREVRRICEFTELEFDPRQVPGPGQRMPLGSLSPEKWYPLKPEENLRYSR
                     DLAPELVAGLNQRAADVIRRLGYEVIE"
     gene            918..2126
                     /locus_tag="VD788_09760"
     CDS             918..2126
                     /locus_tag="VD788_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV76594.1"
                     /translation="MRIALLNQFYPPSDAPTGLLLDDLGRALAASGHRVEAICSDRDY
                     NDPTLRYPAIDQVGGVEVRRVGGTRLGRGSRAGRAIDYLNFLTRAGARLLAGRRADVV
                     VVLTTPPMLAAPVMAVAALKRTRVVFWVMDVYPDLAFELGVIRRESAGGRLLSALSSR
                     LLRAADVVVALGETMATRLGEAGARRVETVHNWADGEAIRPLPTDGHALRRAWGWNAR
                     TVVQYSGNMGLAHEFDTVLDAAAGLLHRPEILFAFVGGGPRRAEVEQQARRRGLRNVE
                     FRPWVAQDRLGESLTAGDVHLVTLRPGLEGLLVPSKIYGILAAGRPTLYVGPARCEVG
                     RIVEQGCCGIRVERGDAAELARCIERYADDRALRDEHGRAARETFERSFDRRRALSRL
                     CAIVTATDAD"
     gene            2152..4119
                     /locus_tag="VD788_09765"
     CDS             2152..4119
                     /locus_tag="VD788_09765"
                     /inference="COORDINATES: protein motif:HMM:NF027452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HXV76595.1"
                     /translation="MATPAPRRYHPRDGADGSIGRMRPGAGMTTPRVGATLLAALAAA
                     CPAPAAAGSIRLAADGQARAAIVVPPSATAAEREAAAELGHYLDRVTGGSFPVVADAE
                     SAPGLAAIRIGWTDDRPAGAPRPEELGPEEWVVRTDERGLAIYGGRPRGTLYGVYHFL
                     EDEIGVEWWTPFDEFVPHRPTLIVEPLDERGAPAFGYRDIAVESGPPRFRARSRLNGH
                     FSRLPLRLGGSLAFAPPSHAHTAFLLVPPELYDDHPELFAEIGGMRFAGDSQLCLSEP
                     AVVDRVVDEVLRHLARARAAAERSGFAAPRLVSVSQNDWERPCECAGCMRTVAQRGSR
                     AATWIVFVNEVARRVAEVEPDVLVETLAYQHTLAPPTGPPAASNVVVRVSGLHQRDLL
                     RPITAADNRVHRAALLGWDGLVEHLFVWDYTVEFGEDGNLPLPNLALFADDLRFYRRV
                     GVEGVYYQPEDPIADDLRDLKIWVLARLIADPSRSVEALVRRFTDGYYGTAGKSIRAY
                     LAVVERAARRADEPLFYGAPASAHPYLDRRFFAHARRLFDRAERRVAERTDLAARVRS
                     ARLALDRATMALWSRACADACGADEFDHLARRYRATWSAELGRRAPTADLDAALAEVD
                     REIAFRRRRADPPAAPAAAAAEPQPRPRNAN"
     gene            4212..5942
                     /locus_tag="VD788_09770"
     CDS             4212..5942
                     /locus_tag="VD788_09770"
                     /inference="COORDINATES: protein motif:HMM:NF004040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional sulfate
                     adenylyltransferase/adenylylsulfate kinase"
                     /protein_id="HXV76596.1"
                     /translation="MHQTLIAPHGGELVDLMVDDVRAASLKEESKDWPSWDLTPRQIC
                     DLELLLCGAFSPLRGFMVRRDYDSVRDRMRLADGTLWPIPITLDVDDRTAARLGAGAK
                     LALRDAEGVMLAVLKVEEIWRPDRDTEAEKVFGTTDAAHPAVRHLHEASHANYVGGAI
                     EGVQPPLHYDFRELRLTPAQVRRRLERFGWLRVVAFQTRNPMHRAHHELTLRAAKEVE
                     ANLLIHPVVGVTKPGDLDHYTRVHCYKAILNRYPQQTVMLSLLPLAMRMGGPREAVWH
                     AIIRKNYGCTHFIVGRDHAGPGNDSTGQPFYGPYDAQQMMVEHQTELGVEMVPFKHMV
                     YVEDRDAYFPADEVPSGSRVLNISGTDLRQRLAEGRDIPEWFTFPDVATQLQRRHPPR
                     FRQGFTVFFTGLSGAGKSTIANALMVKFLEMGGRPVTLLDGDIVRKNLSSELGFSKEH
                     RNINISRIGYVASEITKNGGIAICAPIAPYDAVRKAVREMIAPLGGFILVHVDTPIDV
                     CEDRDRKGLYAKARAGIIKEFTGVSDPYELPEDADLVLDTTRLSPEQAAQQVLLHLER
                     EGYVGVNGDS"
     gene            5942..6733
                     /locus_tag="VD788_09775"
     CDS             5942..6733
                     /locus_tag="VD788_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXV76597.1"
                     /translation="MSRGEDLRRIVEALGAACSILAAFTPGEIVQRSKSGGDPVTEAD
                     LAIDAALRRILPRTNEGWLSEETIDDPERLDRERVWVVDPLDGTKEFVSGIPEWCVSI
                     GLVENSRPVAGGICIPARGLQVVGAVGSGVEVNGEPPRPRAASGLDGAEVLASRSEVD
                     RGEWRRFAGAPFVVRPMGSVAAKLALVAAGLTDATWTLVPKHEWDVAGGAALVLAAGG
                     DVWRLDGSSPRFNQPKPKFDGLFAAAAGLRDPIERYFREIGIRAN"
     gene            6778..8577
                     /gene="asnB"
                     /locus_tag="VD788_09780"
     CDS             6778..8577
                     /gene="asnB"
                     /locus_tag="VD788_09780"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXV76598.1"
                     /translation="MCGINGILRLDARAAPVDREELLTTRDRMAARGPDGSGLWIADD
                     RTIGLGHRRLAIIDLSDAGLQPMATRDGRYRIVFNGEIYNYRELRAELLAAGARFESH
                     SDTEVVLESFARRGVDSFSGLRGMYALAIWDAAERRLVLARDPYGIKPLYYSVSGTHL
                     RFASQLKALEAGGAIGSDVDPAGLAGFLLWGSVPEPFTLRREVRCLPAGHYLVAADGR
                     AGAPQPHARLACASRDDDGSAAVDALEDSVRAHLVADVPVAVFLSSGLDSSLLAALAR
                     RAADDPPTTLTLKFDAYVGSQRDEAPLAAEVARVLGTRHVERFVKREDFLDLWPAALH
                     AMDQPSVDGFNTYVVSQAAHREGFKVVLSGVGGDELLGGYRSFPEVPRWSRWAAALDR
                     LPGVRRVWPAAAGVRPSTPKLAGLIEYGTTLPGAYFVRRALYLPEELGALIGAGTAAD
                     GLAAYDPVADAGKYLGPLPDHDGWQAVHLMESSQYMRNQLLRDSDWASMAHSLELRVP
                     LVDARLQQSFAAADFRPARDGGKAAAIRAAAPELPASLWSRPKTGFSIPVMEWLDERI
                     DPSTAPGLASRALALRVLEAFGVELAERVGRGD"
BASE COUNT         1208 a         3101 c         3123 g         1316 t
ORIGIN      
        1 ggtgcggatc gaccggccgg tcttcctcgt cggcgtccag ggaggcggcg gcacgatcgt
       61 ggcgcgcacg ttgtaccgtc acccgcgcgc ggtctacgcc tcgggcaact cgcgcttctg
      121 ggccggcctc gacgaaattc acaacagccg gcacacgatc cgcgacttgc cggagtcgct
      181 gatccaccgt agcgcccact ttttcaacgt cgacgggcgt ctcgactgcc acccgatcta
      241 cggctaccag cgttgctggt tgtacgcgat cgacgaattc ctcgaccgct atcgcagaac
      301 cgcggccgat gccgacgagt cgacgtcgct cgcgttgcgt cgagtgatcg gcaagacgtt
      361 gctggcctac gcgcacgacc tgcggaccgc ccgcttcgtc gacatgagcc agctctacac
      421 gatccagctg tcctacatcg ccgagctgct gcgcggctgc gacccgcggt tcgtcctgtt
      481 gacgcgcaat ccgtacgtga ccgtcgcgcg cgcggcccgc aaggaatacg acgggcacgg
      541 cacgaaggtc gaaatgacga ccgagcagaa gatgcgctgc gcgatcgagc actggcacaa
      601 ctcgtaccgg ctggccctcg acgacgcccg cggccggccg ctgttgcagg tgaagtacga
      661 ggacttcctc gaggatccgc tgcgcgaggt gcgccgcatc tgcgagttca ccgagctcga
      721 attcgatccg cgccaggtcc cgggacccgg gcagcgaatg ccgctcggct cgctgtcgcc
      781 ggagaagtgg tacccgctga aacccgagga gaacctacgc tactcgcgcg acctcgcgcc
      841 cgagctggtc gccggtctga accagcgggc cgcggacgtg atccgccggc tgggttacga
      901 ggtcatcgag tagcgcagtg cggatcgcgt tgttgaatca gttctacccg ccgtccgacg
      961 cgccgaccgg tctcctgctc gacgacctcg gccgcgcgct cgccgcgagt ggccaccggg
     1021 tggaggcgat ctgctccgac cgcgactaca acgacccgac gctgcgctac ccggcgatcg
     1081 accaggtcgg tggggtcgag gtcaggcgtg tcggcgggac ccggctcggt cgcgggagtc
     1141 gtgccgggcg cgcgatcgac tacctcaact tcctcacccg cgcgggcgcg agactgctcg
     1201 ccggaaggcg ggccgacgtg gtggtcgtgc tgaccacacc gccgatgctg gccgctccgg
     1261 tgatggccgt cgccgcgctc aagcgcacgc gtgtcgtctt ctgggtgatg gacgtctatc
     1321 ccgatctcgc gttcgagctc ggcgtgatcc ggcgcgaatc ggccggtggc cggctgctgt
     1381 ccgcgttgtc gagccggctg ctccgcgcgg ccgacgtcgt ggtcgcgctc ggcgagacga
     1441 tggccacgcg gctcggcgag gccggcgcac ggcgggtcga gacggtgcac aactgggccg
     1501 acggcgaggc gattcgcccg ctgccgaccg acgggcacgc gctgcgtcgg gcctggggct
     1561 ggaacgcgcg gacggtggtg cagtactcgg gcaacatggg gctggcccac gagttcgaca
     1621 cggtgctcga cgccgccgcc ggactgcttc atcgtcccga gatcctgttc gccttcgtcg
     1681 gcggcggacc gcgccgggcg gaggtcgagc agcaggcgcg gcgccgcggg ttgcggaacg
     1741 tcgagttccg cccgtgggtc gcgcaggacc ggttggggga gagcctgacc gccggcgacg
     1801 tccacctggt gactctgcgg cccggtctcg aggggctgct cgtgccgtcg aagatctacg
     1861 ggatcctggc cgcgggccgg ccgacgctct acgtcggtcc cgcgcggtgc gaagtgggcc
     1921 ggatcgtcga gcagggatgc tgcgggatcc gggtcgagcg cggcgacgcc gcggagctgg
     1981 cgcgatgcat cgagcgctac gccgacgacc gagcgctccg cgacgagcac ggccgcgcgg
     2041 cgcgcgagac gttcgagcgg agcttcgatc ggcggcgagc gctttcgcgg ctgtgcgcga
     2101 tcgtcaccgc gacggatgcc gactagccgg ctccgccccg ccgatctcgc gatggcgacc
     2161 cccgcgccgc gtcgttacca tccccgcgat ggtgccgacg gttcgatcgg gcggatgcgg
     2221 ccgggtgccg ggatgacgac gccacgcgtc ggcgcgacgc tgctcgcggc gctggcggcc
     2281 gcctgccccg cgcccgcggc ggcgggcagc atccggttgg cggccgacgg acaggcgcgc
     2341 gccgcgatcg tcgttccgcc gagcgcgacc gcggccgagc gcgaagccgc cgccgagctc
     2401 ggacactacc tcgaccgcgt caccggcggg agcttcccgg tggtggcgga cgccgagtcc
     2461 gcgccgggtc tcgccgcgat ccggatcggc tggaccgacg atcggccggc cggtgccccg
     2521 cgccccgagg agctcggacc cgaggaatgg gtcgtgcgca ccgacgagcg tgggctcgcg
     2581 atctacggcg gcagaccgcg cgggacgctc tacggcgtct atcacttcct cgaggacgag
     2641 atcggcgtcg agtggtggac cccgttcgac gaattcgtcc cccaccggcc gacgctgatc
     2701 gtcgagccgc tcgacgagcg cggggcgccg gcgttcggct accgcgacat cgccgtcgag
     2761 agtggtccgc cgcgcttccg cgcccgcagc cggctcaacg gtcatttcag ccgcctcccg
     2821 ctgcggttgg gcggctcgct cgccttcgct ccgccgtccc acgcgcatac ggccttcctg
     2881 ctcgtcccgc ccgagctgta cgacgatcac cccgagctgt tcgccgagat cggcgggatg
     2941 cgattcgccg gcgacagcca actctgtctc agcgagcccg cggtcgtcga ccgtgtcgtc
     3001 gacgaggtgc tgcggcacct cgcccgcgca cgcgccgcgg ccgagcgctc cggcttcgcc
     3061 gccccgcgtt tggtcagcgt gtcgcagaac gactgggagc ggccctgcga gtgcgcgggt
     3121 tgcatgcgga cggtcgcgca acgggggtcg cgggcggcga cgtggatcgt cttcgtcaac
     3181 gaggtcgcgc gacgcgtggc cgaggtcgag ccggacgtcc tggtcgagac gctggcctac
     3241 cagcacacgc tcgccccgcc gaccggccca ccggccgcct cgaacgtcgt cgtgcgcgtc
     3301 tcggggttgc accagcgcga ccttcttcgg ccgatcacgg cggcggacaa ccgggtgcat
     3361 cgcgcggcgt tgctcggctg ggacgggctg gtcgagcacc tgttcgtctg ggactacacg
     3421 gtcgagttcg gcgaggacgg caacctgccg ctgcccaacc tcgcgctgtt cgccgacgat
     3481 ctgcggttct atcgccgggt cggcgtcgag ggcgtctact accagccgga agacccgatc
     3541 gccgacgatc tgcgcgatct caagatctgg gttctcgcgc ggctgatcgc cgacccgtcc
     3601 cggtcggtcg aggccctcgt acggcggttc accgatggct actacgggac cgccggaaag
     3661 tcgatccgcg cctatctggc ggtcgtcgag cgggccgcgc gccgcgcgga cgaaccgctg
     3721 ttctacggcg cgccggcgtc ggcccatccc tacctcgacc ggcgcttctt cgcgcacgcc
     3781 cggcgactgt tcgaccgtgc ggagcgacgt gtggcggagc ggaccgacct ggccgcgagg
     3841 gtgcggagcg cccggctggc cctcgatcgg gcgacgatgg cgctgtggtc gcgtgcctgc
     3901 gccgacgcct gtggtgcgga cgagttcgat cacctggccc gccggtaccg cgcgacctgg
     3961 tccgccgagc tcggccgccg ggctcccacg gccgacctcg acgccgcgct ggcggaggtc
     4021 gatcgtgaga tcgcgttcag acggcggcga gcggacccac ccgcggcccc cgcggccgcc
     4081 gccgccgagc cgcaaccgcg gccaaggaac gcgaactagt cggcgatgac gcgtttgaca
     4141 ccctccggtc cgacagatat cgtcccgcgg atcggcggat cgacactcga tcgcgcgagg
     4201 ggaaggctcc gatgcaccaa accttgatcg cgccgcacgg tggcgagctc gtcgatctga
     4261 tggtcgacga cgtccgcgcg gcttcgctga aggaagaatc gaaagactgg ccgtcgtggg
     4321 atctcacgcc gcggcagatc tgcgacctcg aactgctgtt gtgcggcgcg ttctctccgc
     4381 ttcgcggatt catggtccgc cgggactacg acagcgtccg cgaccgcatg cggctggccg
     4441 acggcacgtt gtggccgatc ccgatcacgc tcgacgtcga cgatcgaacc gcggcccggc
     4501 tcggtgccgg cgccaagctg gcattgcgtg acgcggaagg cgtgatgttg gcggtgctga
     4561 aggtcgagga gatctggcgg ccggaccgcg acaccgaagc cgagaaggtc ttcggcacga
     4621 ccgacgccgc ccacccggcc gtccgtcacc tgcacgaggc gagccacgcg aactacgtcg
     4681 gcggcgcgat cgagggtgtg cagccgccgc tgcattacga ctttcgcgag ctgcggttga
     4741 cgccggcgca ggtgcgccgg cgtctcgaac gcttcggctg gctgcgcgtc gtcgcattcc
     4801 agacacgcaa tccgatgcac cgcgcgcacc acgagctgac gctgcgcgcg gcgaaggagg
     4861 tcgaggcgaa cctgctgatc cacccggtgg tcggcgtgac caagccgggc gacctcgatc
     4921 attacacgcg cgtgcactgc tacaaggcga tcctgaaccg ctacccgcag cagaccgtga
     4981 tgctgtcgct gctcccgttg gcgatgcgga tgggcgggcc gcgcgaggcg gtctggcacg
     5041 cgatcatccg caagaactac ggctgcacgc acttcatcgt cggtcgcgat cacgcaggtc
     5101 cgggcaacga ttcgacgggc cagccgttct acggcccgta cgacgcacag cagatgatgg
     5161 tcgagcatca gaccgagctc ggcgtcgaaa tggtcccgtt caagcacatg gtctacgtcg
     5221 aggaccgcga cgcgtatttc cccgccgacg aagtaccgtc cggctcgcgc gtgctcaata
     5281 tttccgggac cgatctgaga caacggctgg ccgagggtcg cgacatcccc gagtggttca
     5341 ccttccccga cgtggcgacc cagctgcagc gccgtcatcc gccgaggttc cgccagggat
     5401 tcaccgtctt cttcaccggg ctgtcgggcg cgggcaagtc gacgatcgcc aatgcgttga
     5461 tggtgaagtt cctcgagatg ggcgggcgcc cggtgacgct gctcgacggg gacatcgttc
     5521 gcaagaacct ctcgtccgag ctcggcttct ccaaggagca ccgcaacatc aacatcagcc
     5581 gcatcggcta cgtcgcctcc gagatcacca agaacggcgg gatcgcgatc tgcgccccga
     5641 tcgcgccgta cgacgcggtg cgcaaggccg tgcgcgagat gatcgccccg ctcggcggct
     5701 tcatcctggt gcacgtcgac accccgatcg acgtctgcga ggaccgcgat cggaaagggc
     5761 tctacgccaa ggcgcgggcc gggatcatca aggagttcac cggcgtctcc gacccctacg
     5821 agctccccga ggacgccgac ctggtgctcg acaccacgcg cttgtcgccc gagcaggcgg
     5881 cacagcaggt gctgctgcac ctcgagcgcg agggctacgt cggcgtcaac ggcgacagct
     5941 gatgtcgcgc ggggaggatc tgcgccggat cgtcgaagcg ctcggcgcgg cgtgctcgat
     6001 cctcgccgcg ttcacgccgg gcgagatcgt ccaacgatcc aagtccggcg gggatccggt
     6061 gaccgaggcc gatctggcga tcgacgcggc gttgcggcgg attctcccac ggacgaacga
     6121 ggggtggctg tccgaggaga cgatcgacga tcccgagcgg ctcgatcgcg agcgggtgtg
     6181 ggtcgtcgat ccgctcgacg gcacgaagga gttcgtcagc ggcatccccg agtggtgcgt
     6241 ctcgatcggc ttggtcgaga actcccgccc ggtagccggt ggtatctgca ttccggcgcg
     6301 cggcctgcaa gtcgtcggcg ccgtcgggag cggcgtcgag gtcaacggcg agccgccgcg
     6361 tccgcgcgcc gccagcggtc tcgacggagc cgaggtgctc gccagccgca gcgaggtcga
     6421 tcgcggcgag tggcggcgtt tcgccggcgc cccgttcgtc gtgcggccga tgggctcggt
     6481 cgcggccaag ctggcactgg tcgcggccgg actgaccgac gccacctgga cgctcgtgcc
     6541 caaacacgag tgggacgtcg ccggcggagc ggcgctggtg ctggccgccg gcggcgacgt
     6601 ctggcggctc gacggctcgt ccccgcggtt caaccaaccg aagccgaagt tcgacggcct
     6661 gttcgccgcc gccgccggcc tgcgcgaccc gatcgagcga tacttcaggg agatcggcat
     6721 ccgcgcgaac tgagggatcc tcgccgcgcg ggaccggtcg agggaggcga gcgcgccatg
     6781 tgcgggatca acgggattct gcggctcgat gcgcgggccg cgccggtcga ccgcgaggaa
     6841 ctgctcacga cgcgcgaccg gatggccgcg cgtggccccg acggcagcgg tctctggatc
     6901 gccgacgacc gcacgatcgg tctggggcac cggcggctcg cgatcatcga cctgtccgac
     6961 gccggcctgc agccgatggc gacccgcgac gggcgctacc gcatcgtgtt caacggcgag
     7021 atctacaact accgcgagct gcgcgcggaa ctgctcgccg cgggcgcgcg tttcgaatcg
     7081 cacagcgaca ccgaggtcgt gctcgagtcg ttcgcgcgtc ggggcgtcga ctcgttctcc
     7141 ggcctgcgcg gcatgtacgc gctcgcgatc tgggacgcgg ccgaacggcg cctggtactg
     7201 gcgcgggatc cctacggcat caagccgctg tactactccg tctccggcac ccacctgcgc
     7261 ttcgcctcgc agctcaaggc gctcgaggcc ggcggcgcga tcggcagcga cgtcgatccg
     7321 gcggggctcg ccggcttcct gctctggggc tccgttcccg agccgttcac gttgcgacgc
     7381 gaggtccgct gcctcccggc cgggcactac ctggttgccg ccgacggtcg tgccggcgcg
     7441 ccgcagcctc acgcgcggct ggcctgcgcc agccgcgacg acgacggatc cgcggcggtc
     7501 gacgcgctcg aggacagcgt gcgcgcgcat ctcgtcgccg acgtaccggt cgcggtcttc
     7561 ctgtcgtcgg gcctcgattc gagcctgctc gcggcgctcg cgcgacgagc ggccgacgac
     7621 ccgccgacga cgctgacgct caagttcgac gcctacgtcg gcagccagcg ggacgaagcg
     7681 ccgctcgccg cggaggtcgc gcgggtgctc ggcacgcggc acgtcgagcg cttcgtcaag
     7741 cgcgaggatt tcctcgacct gtggcccgcc gcgctccacg cgatggacca gccgagcgtc
     7801 gacggcttca acacctacgt cgtcagccag gcggcgcatc gcgagggctt caaggtcgtg
     7861 ctctccggcg tcggcggcga cgagctgctc ggtggctatc gttcgttccc cgaggtgccg
     7921 cgctggagcc gctgggccgc ggcgctcgat cggctgcccg gcgtgcgtcg cgtctggccg
     7981 gcggcggcgg gtgtccggcc gtcgaccccc aagctcgccg gactgatcga gtacgggacg
     8041 acgctacccg gcgcatactt cgtccggcgc gcgctctact tgcccgagga gctcggggca
     8101 ttgatcggtg cgggcacggc cgccgacggg ctcgcggcct acgacccggt ggcagacgcc
     8161 ggcaagtacc tcgggccgct gccggaccac gacgggtggc aggccgtgca cctgatggaa
     8221 tcgtcccagt acatgcgaaa ccagctgttg cgcgattcgg actgggcgtc gatggcccac
     8281 tcgctcgagc tgcgggtccc gctggtcgac gcccggctgc agcagagctt cgccgccgcc
     8341 gacttccggc cggcacgcga cggcggcaag gccgcggcga tccgcgccgc ggctcccgag
     8401 ctgccggcga gcctgtggag ccgccccaag acgggcttct cgatcccggt gatggagtgg
     8461 ctcgacgagc ggatcgaccc gagcacggcg ccgggactcg cgtcgcgcgc gctggcgctg
     8521 cgcgtcctcg aggcgttcgg cgtcgagctg gccgagcggg tcggccgcgg tgactgattc
     8581 cctcaaacga cggttgctca ccggcggcgt gtgggtcctg gccggcaagc tggtgacggc
     8641 gctggccaac gtcggcgtgc tcgcgctgct cgcgcgactg ctcagcgagc agggcttcgg
     8701 cgactacggc ttggtcttca gtctggtcac cgtcgccgcg gtcgtctc
//
LOCUS       DASPGN010000341        12535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_60395, whole genome shotgun sequence.
ACCESSION   DASPGN010000341 DASPGN010000000
VERSION     DASPGN010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12535
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60395"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VD788_09785"
     CDS             complement(<1..1117)
                     /locus_tag="VD788_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HXV76599.1"
                     /translation="MRSRSIALAWTGLSLVACGATSPPAEVGVDAAPMFDERAHASGI
                     DFVHFNGMSGELYFVEIFGPGVGLFDYDNDGDLDLYVTQGRMLGPGKTVGDAIFPPAD
                     DAPPGDRLFRNELHELGRLTFTDVTDAAGIAPAGYGMGVAAGDYDGDGWIDLYVTAFG
                     PNRLLHNRGDGTFVDRTDASGTQDDRWSTSAAFVDYDADGRLDLYVVNYVSFSFATHR
                     PCVSATGAPGYCGPDSFRPEPDRLLRNLGGGRFADVSEASGVGTLAGSGLGVCYGDFD
                     RDRRLELYVANDLMANRLWAPDGGGRLIDRALLAGCAFNADGQAESSMGVDAGDFDGD
                     GDEDLFVTHLDNETNTLYLNDGRGWFTDVTFERGLGTA"
     gene            1295..1663
                     /locus_tag="VD788_09790"
     CDS             1295..1663
                     /locus_tag="VD788_09790"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HXV76600.1"
                     /translation="MEQRKGQEEKRKPALDAADLERMRPYAPAWLVKPRRGVPALHRT
                     FTFGSFAEAMWFTLKLGEWAERDGQRPTILISWDRVTVALGSAASGGLDRRDFLAAGN
                     VDRLFDGLLRSKRPTGAAGV"
     gene            1702..2205
                     /locus_tag="VD788_09795"
     CDS             1702..2205
                     /locus_tag="VD788_09795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXV76601.1"
                     /translation="MPIYEFYCVDCHRVFSFLSRRVRPRRRPDCPDCGRKRIERRASA
                     FAVAGGRAEPPAETGGEIDDSRVERAMASLAADAERIGDDDPRAMAGLVRRMYDASGL
                     PLGPGMDEAIRRMESGEDPDRIEDEMGEILEAEDPFADGPEEVRPGGAAARRLPPPTV
                     DTTLYEL"
     gene            complement(2216..3349)
                     /locus_tag="VD788_09800"
     CDS             complement(2216..3349)
                     /locus_tag="VD788_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXV76602.1"
                     /translation="MNRYPSTLRFLAALALSAIALPVSAGSQRAETVLNYEGDLRPGQ
                     SLQVDNLVGSLIVRGDDTGGRVLIEARVIAEAATEEEAEALRDAIALRPTDGDGRRSI
                     TVEYPVRRHSGFRLPRSEREGVMDKWVTPLVRKSVVSTEYDGQTVEVGGSKGSVAIAV
                     HVKVTVPMDVRASFRQVVGSLHGIGLRGDLAFEAVDGEILTEQIYGALEVRTGGGQVT
                     VRKFGGETFRLQTASGNVTLIDVTADEAELLSGAGKIEADGITAAKLAVDSGAGAVLL
                     DRVDSETIRVDSESGVIDVAARLTRTREASIISRVGDVTLRVNPTTPFELKAEAESGS
                     VRHRDLAAEVIDEEKNSVHLTRGKGGAAVEIRTGKGAVTIRAI"
     gene            3578..5734
                     /locus_tag="VD788_09805"
     CDS             3578..5734
                     /locus_tag="VD788_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXV76603.1"
                     /translation="MRKLQPALLTILLAAPLAAAPKEDLKLDYPDTKKVEVVDDYHGT
                     KVEDPYRWLEQDARESTDVQRWIEAENEVTFDYLERIPERKAIENRLTELWDYERYSS
                     PFKVGGRYYYAHNSGLQNQSVVYTQDNLDAEARVLLDPNTWSEDGTVALAGLSFSDDG
                     NFMAYAKSVAGSDWQRWYVRDLRTDQDLADELQWTKFTNAAWTPDGRGFFYSRFDAPP
                     PGEEYKALNTDQKVFYHRVGTPQSEDVLVFHRPDQPKWGYGSQVTDDGHYLVISVYEG
                     TDNRNRVFYKDLFEPYGMPVALIPQIDNEYSFLGNDGPIFYFRTDLDAPKGRVIAIDT
                     RRPEPASFRQIIPEADGPLGNVSLKGNMFVVSYLEDVVGRVRVFRTDGTHVRDVELPG
                     KGSVGGFGGQRNDLDTFYTYTSFNTPPTIYRYDLVTGASTLWRKPDLKFDPDAYVVKQ
                     VFYQSKDGTKIPMFITHRKGIELDGNRPTLLYGYGGFSISLTPSFSVTRLAWMEMGGV
                     YAVANLRGGGEYGEEWHDAGRLHNKQNVFDDFIAAAEWLIDNDYTSAEKLAIMGGSNG
                     GLLVGACMTQRPDLFAVAIPAVGVMDMLRYNQFTAGRYWVDDYGNPSEPEYFKTLLAY
                     SPYHNIKPGTSYPATLITTADTDDRVVPAHSFKFAARLQEAHRGDAPVLIRIETRAGH
                     GGGKPTTKQIEETADVYAFLVENLYEESPVIESRTE"
     gene            5882..6352
                     /locus_tag="VD788_09810"
     CDS             5882..6352
                     /locus_tag="VD788_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXV76604.1"
                     /translation="MDDRPERGSQPPRLEQTLDDHRAAMQVVAGVEDCLDRHPDRDGE
                     WIETLRSELRRLIETLRAHFSAEEDGPLYRALPVSHPRFATRLRTLEAEHGRILDRAA
                     QAVIRADRLSEPRLYEIRELNADLQLLVATIRRHEAEENEIVLSAHWDEVGIGD"
     gene            6546..8414
                     /locus_tag="VD788_09815"
     CDS             6546..8414
                     /locus_tag="VD788_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXV76605.1"
                     /translation="MSPRIVDQHIVEFVSDSGEGAQTAGQMFGTVSARMGNGVWTVEI
                     IPAEIEPPHRSQAGASGNRIRIATGEVTNMGEAADVVVAFNEQVLYSRIDVGALRPGT
                     VIFLESAWADAPEPAVREAYAAALRDFAERGFEVVETPMERECKKIVADPRRGKNMWA
                     LGLLCAVYSRDLAIVFDELEKKLGRKGAEVVESNRRLVLSGRDWALEHVPLRYEVPVE
                     PRTQPRVVMNGNQAVALGMMAAGIEVVAMYPITPATSVSHYLAASFHRVGGFLHQAED
                     EISAIGYALGASYAGKTAATVTSGPGLALKTEFLGLAVMAELPLVLVVVQRGSPSTGL
                     PTRVEQGDLLAAVHGEPGDAPKIVMAASTIEECFQFVITARKLAETFRGPVVVLTDAN
                     LATGQTSFRLPEPHADWLSPPVDRSAWTAAVRPYEWDPTTGLSQRPVPGQRGGEYVLT
                     GLAHDEASRVAYESAVNQRAMRMRSRKLITLQKSLRPPEPFGDPQGELLVVGWGSTRG
                     AIEEAVSRLRARGERVSSLTLRFLSPLEPGLAEIFARFAKVMTIEINYSDDADDPEVP
                     PAFRRRSQLARLLRSHTLVDVDCWSRVPGVPLAPNQIEEVLHDRLAASRRGAQCSG"
     gene            8402..9355
                     /locus_tag="VD788_09820"
     CDS             8402..9355
                     /locus_tag="VD788_09820"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXV76606.1"
                     /translation="MFRLKEDWELEPKPRCLVLEDYVGATPRWCPGCGDHAVLGALQR
                     LARDEQLPPEQTVVVSGIGCSSRFPHYMKTYGFHGLHGRPLPVACGIRARRPDLHVFV
                     VTGDGDCCAIGTAHWIHAVRYNMRMTVMLLDNHVYGLTKAQSSPTSRLGQKSNTHPFG
                     APLRPLNPLTVTLGITNASFVAQTLDWNPPHLYATLAEAHRHRGLSFVRVLQRCPHYL
                     EGAWDGVQRDPTRVLLLTHANGIPLDESLARPFTNRVEHDPSDLARGRELAAREDVVP
                     IGLFFRDEDAERYDELSAAGLGMSTAEKLGRIEAELERFVI"
     gene            9371..>12535
                     /locus_tag="VD788_09825"
     CDS             9371..>12535
                     /locus_tag="VD788_09825"
                     /inference="COORDINATES: protein motif:HMM:NF028456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76607.1"
                     /translation="MKTTDSERTGETAARARQESAAARDGARIFASTSAAIDRTERLL
                     CGDGVVRSVAVAEGLALAGSHAATSLGAGAPRPGPSVPRSEAAWVHHRVAGPGTALRG
                     AAAFELLAADAGEAVAHTLAARRMVRLLGRPGICSIEAAAADRPCHVRLPDAREVSAV
                     LDDRSTCAGGVSAAADRVLGRRGILPTYRVDDATLLLVAAGEDRLRAHRLVDRLRRRG
                     LTCGVVGVAMVHPFPAAELRAALAGCESVWVVTPAVGEDAAEAARAAGCAAIVDHRSA
                     GGELLLPPELAERFPANAGRAAVATHEADDRSAVALAVDPPDPEALRAAVAAASEAGW
                     AEPWNVIRLSPGADPAGVVLGRDSTCAPPARIDVGVIVRPAADDGRRAGALLSRLDRR
                     STLLLVTDDDRPLDTWRALGPARRAAITGGRPATRWISMADWSTVSADLARLIRERFA
                     GARSQPADGTLRSIPIDVETVEAELRAEAATVGGEAASPVMPEVALVERSSVESWEFH
                     RTAPRCFDERPLGGPLVPWAAVGLGAATPEWRAYPFVLDARGVGAGHAPLDVLLQEAI
                     EHAGDGLSSGILSRRRPTVLRLANRTASASGSLAPPAELVRAALSALPDELDLSEAGR
                     AELQREIATVEPRMPAGGRAVGFDERTLDILLAETLVARRAPARAEFRRCVEQLVERL
                     DELLRIDDGHRPQAASPAAIAPTLGAGERFVDPERLSRHLPAYRGSAPLEPERRARIE
                     RSRATLRSWLDAGGSSADLILVQTTALGPDPRPRCRVVVHPDPLRAAVGLWDGLARSM
                     VEPVRALRTARLDVSGHYEPERHDRPIAALDPLGFSAEDMLQIPAVVVRDDERPLGED
                     ALGAFSALLRSGRPVHVLATRRATDVSLSRDTGPGYDPGTGYLALAHREAFVIQTSLT
                     RPATLTEAFERMAIGLRPSVALVTEPDWDGPIEPWRQLVLAEFGRDPYHFRYDPAAGP
                     SWAERFDIDDNPQPAAGWPRHSVEQAGTARGASVVEPCFTMADLAAAVPRSRGHFRTL
                     SANEWASDQTEIGSYCELEPTEQLR"
BASE COUNT         1929 a         4315 c         4369 g         1922 t
ORIGIN      
        1 tggcggtgcc cagcccgcgc tcgaaggtca cgtcggtgaa ccatcctcga ccgtcgttga
       61 ggtagagcgt gttcgtctcg ttgtcgaggt gcgtgacgaa cagatcctcg tcgccatcgc
      121 cgtcgaagtc tccggcgtcg acccccatgc tcgactcggc ctgtccgtcg gcgttgaacg
      181 cgcagccggc caagagcgcc cggtcgatca accgcccgcc gccgtcgggt gcccacagtc
      241 gattcgccat caagtcgttg gcgacgtaca gctcgagtcg ccggtcgcga tcgaaatcgc
      301 cgtagcacac gccgagaccg gaccccgcga gcgtgccgac gccggacgct tcgctcacgt
      361 cggcgaaccg tccgccgccg aggtttcgca acagccggtc gggctccggc cgaaaactgt
      421 ccgggccgca atagcccggg gcgccggtcg ccgacacgca cggccggtgc gtggcgaagg
      481 agaaggagac gtagttcacg acgtagagat cgagccggcc atcggcgtcg tagtcgacga
      541 aagcggcact cgtgctccac ctgtcgtcct gcgttccgct cgcatcggtc cgatcgacga
      601 acgtcccgtc gccgcggttg tgcagcagcc ggtttggacc gaaggcggtg acgtacagat
      661 cgatccaacc gtcgccgtcg taatcgcccg ccgcgacacc catgccgtaa ccggccggtg
      721 cgattcccgc ggcgtccgtc acgtcggtga acgtcaaccg gcccagctcg tgcaactcgt
      781 tgcggaacag gcgatcaccg gggggcgcat cgtccgccgg cgggaagatc gcgtcgccca
      841 cggtcttgcc gggaccgagc atccgaccct gggtcacgta gaggtcgaga tcaccgtcat
      901 tgtcgtagtc gaacagcccg accccaggcc cgaagatctc gacgaagtac agctcgcccg
      961 acatgccgtt gaagtgcacg aagtcgatcc ccgaggcgtg ggcgcgctcg tcgaacatcg
     1021 gcgccgcgtc gaccccgacc tccgccggcg gcgacgtcgc gccgcacgcc acgagcgaca
     1081 gtccggtcca tgccagcgcg attgaccgtg atcgcatgcg gaatgccccg tcagcgagca
     1141 agggagcacc agaacatgcc acgtctcgtc ggcgcgggca acgctcatcg ccggtggccg
     1201 ggttgtcggt cttttctggg cgcgaaacgg cgtggttacg agattcgcgg tggtcaagtc
     1261 tggcacgcgt cgtaacgtgg tcgcggggcc agcgatggaa caacgcaaag gtcaagaaga
     1321 aaaacgaaag cccgcgctcg acgccgccga tctcgaacgc atgcgtccgt acgccccggc
     1381 ctggctggtc aagccgcgcc ggggggtacc ggcgctccac cggacgttca ccttcggcag
     1441 cttcgccgag gcgatgtggt tcacgctgaa actcggtgag tgggccgagc gcgacgggca
     1501 gcgtccgacg atcctgatca gctgggaccg cgtgacggtc gcgctgggat cggccgcatc
     1561 gggcggcctc gatcggcgcg acttcctcgc cgctggaaac gtcgatcgcc tgttcgacgg
     1621 gttgctccga tccaaacggc cgaccggcgc ggccggcgtc tgaggatggt ccccgaccgg
     1681 cgattcgcct agaatcaccg catgccgatc tacgagttct actgcgttga ttgccatcgt
     1741 gtcttcagct tcctgtctcg ccgcgtgcgg ccgcgccgcc gccccgactg cccggattgc
     1801 ggccgcaagc gaatcgagcg gcgcgcgtcg gcgttcgcgg tggccggcgg tcgggccgaa
     1861 ccgccggccg agaccggcgg cgagatcgac gacagccgcg tagaacgcgc gatggcctcg
     1921 ctcgccgccg acgcggagag gatcggcgac gacgatccac gggcgatggc cggtctggtg
     1981 cgcaggatgt acgacgcctc gggtctgccg ctcggccccg gcatggacga ggcgatccgt
     2041 cggatggagt ccggggagga tccggaccgc atcgaggacg agatggggga gatcctcgaa
     2101 gcggaggacc cgttcgccga cgggccggaa gaggttcggc cgggaggggc ggcggcccga
     2161 cgccttccgc cccccaccgt cgacaccacg ctctacgagt tgtgacggcc cgcgatcaga
     2221 tcgcgcggat cgtcaccgcg cccttgcccg tgcggatctc gaccgcggcg ccgcccttgc
     2281 cccgcgtcag atggaccgag ttcttctctt cgtcgatgac ctcggccgca aggtcccgat
     2341 gcctgaccga tccgctctcg gcctcggcct tgagctcgaa cggagtggtc ggattgacgc
     2401 gcaaggtgac gtcgccgacg cgtgagatga tcgacgcctc gcgcgtgcgt gtcagacgcg
     2461 ccgcgacgtc gatcactccg gactccgagt cgacccggat cgtctccgag tcgacccgat
     2521 cgagcagcac cgctccggcg ccactgtcga cggccaactt cgccgcggtg atgccgtcgg
     2581 cctcgatctt gcccgcaccg ctcaacagct cggcctcgtc ggccgtgacg tcgatcagcg
     2641 tgacgttgcc cgacgcggtc tgcagccgga acgtctcgcc gccgaatttc cgcaccgtca
     2701 cctgaccacc gcccgtgcgc acctcgagcg cgccgtagat ctgctcggtg aggatctcgc
     2761 cgtcgaccgc ctcgaacgcg agatcgccgc gcagtccgat cccgtgcagg ctgccgacca
     2821 cttggcggaa cgacgcgcga acgtccatcg ggacggtcac cttgacgtgg accgcgatcg
     2881 cgaccgagcc cttcgatccg ccgacctcga cggtctgacc gtcgtactcg gtcgacacga
     2941 cgctcttgcg cacgagcggc gtgacccact tgtccatcac cccttcgcgc tcgctgcggg
     3001 gcaggcgaaa tccgctgtgt cggcggaccg ggtattcgac ggtgatcgag cgccggccgt
     3061 cgccgtcggt cggacgcaac gcgatcgcgt cgcgcagcgc ttcggcctct tcctccgttg
     3121 cggcctcggc gatcacgcgg gcctcgatca gcaccctgcc gccggtgtcg tcgccccgca
     3181 cgatcagcga gccgaccagg ttgtcgacct gcagcgattg accgggacgc aggtcgccct
     3241 cgtagttcaa cacggtctcg gcccgttggc tgccggccga gacggggagc gcgatcgcgg
     3301 acagcgcgag cgcggccagg aatctcaggg tggacggata tcggttcatc ggacaatcct
     3361 cccggtcgca gaagataggt tccgagcacg accgaggcca cgagccgtgc cgcggccggc
     3421 cggtgcgcca cacgccgccg gcccggggcg gattacgatc cggcaagccc gaccacgcga
     3481 gcgtgatcgc tcggtcccgg gaaaccgcgc gggccgccgt tcgtacctac cctctgctag
     3541 gatgggggaa tcctggacgg cttctcggag gatgacgatg cgcaagctcc agcccgcact
     3601 cctgacgatt ctgctggccg ccccgctggc ggccgccccc aaggaagacc tgaagctcga
     3661 ctaccccgac accaagaagg tggaggtcgt cgacgactac cacggcacga aggtcgaaga
     3721 cccgtaccgc tggttggagc aggacgcccg cgagtcgacc gacgtccagc gctggatcga
     3781 ggcggagaac gaggtcacct tcgactacct cgagcgaatc ccggaacgca aggcgatcga
     3841 gaaccggctg accgagctgt gggactacga gcgctactcg tcgccgttca aggtcggcgg
     3901 gcgttactac tatgctcaca acagcgggtt gcagaaccaa tcggtggtgt acacccaaga
     3961 caatctcgac gccgaggccc gcgtgctgct cgacccgaac acctggtccg aggacggcac
     4021 cgtcgcactg gccgggctgt cgttcagcga cgacggcaac ttcatggcgt acgccaagtc
     4081 ggtcgccggt tccgactggc agcgctggta cgtgcgcgat ttgcgaaccg accaggacct
     4141 ggccgacgag ctgcaatgga ccaagttcac caacgcggcg tggacgccgg acgggcgcgg
     4201 cttcttctac agccgcttcg acgcgccgcc gccgggtgaa gagtacaagg cgctcaacac
     4261 cgaccagaag gtgttctacc accgcgtcgg tacgccgcag tcggaggacg tgctcgtctt
     4321 ccatcggccg gatcagccga agtggggcta cggcagccag gtgaccgacg acggtcacta
     4381 cctggtcatc agcgtctacg agggtaccga caatcggaac cgcgtcttct acaaggacct
     4441 gttcgagccc tacggcatgc cggtcgcgct gatcccgcag atcgacaacg agtactcgtt
     4501 cctcggcaac gacgggccga tcttctactt ccgtaccgat ctcgacgctc ccaagggacg
     4561 cgtgatcgcg atcgacacgc ggcggcccga gccggcgagc ttccggcaga tcatcccgga
     4621 ggccgacggt ccgctgggca acgtgtcgct caagggcaac atgttcgtcg tcagttatct
     4681 cgaggacgtc gtcggccgcg ttcgtgtttt tcgcaccgac ggtacgcacg tgcgcgacgt
     4741 cgagttgccc ggtaagggat cggtcggcgg gttcggcggg cagcgcaacg acctggacac
     4801 gttctacacc tacaccagtt tcaatacgcc gccgacgatc taccgctacg acctggtgac
     4861 cggcgcgagc acgctgtggc gaaagccgga tctcaagttc gatcccgatg cgtacgtcgt
     4921 caagcaggtg ttctaccaga gcaaggacgg aacgaagatc ccgatgttca tcacccaccg
     4981 caaggggatc gagctcgacg gcaatcgtcc gaccctgctc tacggctacg gcggcttcag
     5041 catctcgttg acgccgtcgt tctcggtcac ccggctggcg tggatggaga tgggcggggt
     5101 ctatgcggtg gccaatctgc gcggcggcgg cgagtacggc gaggaatggc acgacgccgg
     5161 ccgcctgcac aacaagcaga acgtgttcga cgacttcatc gccgccgccg agtggttgat
     5221 cgacaacgac tacaccagcg cggagaagct ggcgatcatg ggcgggagca acggcgggct
     5281 gctggtcggc gcctgcatga cgcagcggcc ggatctgttc gccgtcgcga tcccggcggt
     5341 cggcgtgatg gacatgctcc gttacaacca gttcaccgcc ggtcgctact gggtcgacga
     5401 ctacggcaat ccgagcgagc cggagtactt caagacgctg ttggcctact cgccttacca
     5461 caacatcaag cccggtacga gttatccggc gacgctgatc acgacggccg acaccgatga
     5521 tcgcgtggtc ccggcccaca gcttcaagtt cgccgcccgc ctgcaagagg cccaccgcgg
     5581 ggacgcgccg gtcctgatcc gcatcgaaac ccgcgccgga cacggcggcg ggaagccgac
     5641 gacgaagcag atcgaggaga ccgccgacgt ctacgctttt ctcgtcgaga atctctacga
     5701 agaatcaccg gtgatcgaga gccggaccga atgaccgcga gtcgagtgcg ttcatcgacg
     5761 gcctccgcga ggaggccgtt tttttgggtt ctgaatggac tgcgacactc ctttattgga
     5821 gttgtgacgt ggcgctgact ccccgaacat aacgaagagg cgacgcatcg acgggggagt
     5881 gatggacgac aggcccgaac gcggctcgca gccgccccga ctggagcaga cactggacga
     5941 ccaccgggcg gcgatgcagg tcgtcgccgg cgtggaggac tgtctcgatc gtcacccgga
     6001 tcgggacggc gagtggatcg aaacgctgcg gagcgagctc cgccggctga tcgagacgct
     6061 gcgcgctcac ttcagcgccg aggaggacgg accgttgtat cgggcgctgc cggtctccca
     6121 cccacgcttc gcgacgcgct tgcgcacgct cgaagccgag cacggtcgga ttctcgatcg
     6181 cgcggcacag gccgtgatcc gtgcggaccg tctcagcgag ccacggctgt acgagatccg
     6241 cgagctgaac gccgacctgc agctgctggt ggcgacgatc cgacgccacg aggcggaaga
     6301 gaacgagatc gtcctgtcgg cgcactggga cgaggtcggc atcggcgact gagcgtcgga
     6361 aaagcgaccc gaaccatccc tttgcgcggt ggtgccggct gtcctcgacg aaacagaatg
     6421 gggacgatgg aaatcgggcc gggttcccat cgacggtagc gaggatcgaa agatcgtaat
     6481 cgccgtggcg cgatcccgcc ccgatcagcc ggacgaccat cgatccgtcc gaaggaggcc
     6541 atcggatgtc gccgcgcatc gtcgatcagc acatcgtcga gttcgtgtcg gactccggcg
     6601 aaggggccca gactgccgga cagatgttcg gcaccgtcag cgcgcggatg ggcaacggcg
     6661 tgtggacggt cgagatcatt ccggccgaga tcgaaccacc gcaccgctcc caggcgggcg
     6721 ccagcggcaa ccgcatccgc atcgccaccg gcgaggtgac gaacatgggc gaggcggccg
     6781 acgtggtcgt cgcgttcaac gagcaggtgc tctacagccg catcgacgtc ggggcgttgc
     6841 gcccgggtac ggtgatcttc ctcgagagtg cgtgggccga cgcgccggag ccggcggtgc
     6901 gcgaggccta cgcggcggcg ctgcgcgact tcgccgaacg cggcttcgag gtggtcgaga
     6961 cgccgatgga gcgggagtgc aagaagatcg tcgccgaccc gcgccgcggc aagaacatgt
     7021 gggcgctcgg gctgctgtgc gcggtctact cccgcgatct cgccatcgtc ttcgacgaac
     7081 tggaaaagaa gctgggtcgc aagggagcgg aggtcgtcga gtcgaaccgc cggctggtgc
     7141 tgtcggggcg agactgggcg ctcgagcacg tgccgttgcg ctacgaggtg ccggtcgaac
     7201 cgcgcaccca gccgagggtg gtgatgaacg gcaaccaggc cgtcgcgctc gggatgatgg
     7261 ccgccggaat cgaggtcgtc gcgatgtatc cgatcactcc ggcgacttcg gtctcgcact
     7321 acctggcggc gagcttccat cgtgtcggcg gcttcctgca ccaagccgag gacgaaatct
     7381 ccgcgatcgg ttatgcactg ggcgcgagct atgcgggcaa gaccgcggcg accgttacgt
     7441 cgggtcccgg gctcgcgctg aagaccgagt tcctcgggct ggcggtgatg gccgagctgc
     7501 cgctggtgtt ggtcgtggtt cagcgcggca gcccatcgac cgggctgccg acccgcgtcg
     7561 agcagggcga cctgctggcg gcggtgcacg gcgagccggg cgacgcgccc aagatcgtga
     7621 tggccgcctc gacgatcgag gagtgcttcc agttcgtcat caccgcgcgc aagctggccg
     7681 agacctttcg cggaccggtg gtcgtgctga ccgacgccaa cctggcgacg ggccagacgt
     7741 cgttccggct gcccgagccg cacgccgact ggctgagccc gccggtcgat cggtcggcgt
     7801 ggaccgccgc cgtccgaccg tacgagtggg atccgacgac cggcttgtcc cagcgaccgg
     7861 tgcccggaca gcggggcggc gagtacgtgc tcaccggtct ggcccacgac gaggcgagtc
     7921 gcgtggccta cgagtccgcc gtcaaccagc gcgcgatgag gatgcgcagt cgcaagctga
     7981 tcaccctgca gaagtcgctt cgtccgcccg agccgttcgg tgacccgcag ggcgagctgt
     8041 tggtcgtcgg ctggggttcg acgcgcggcg cgatcgagga agcggtctcg cgcctgcgcg
     8101 ctcgagggga gcgtgtctcg tcgttgactc tccgcttcct ctctccgctc gaaccaggtc
     8161 tggcggagat cttcgcccgc ttcgccaagg tgatgacgat cgagatcaac tacagcgacg
     8221 acgcggacga tccggaggtt ccgccggcgt tccgtcggcg atcgcagctc gcacgcctgc
     8281 tgcgctccca cacgctggtc gacgtcgact gctggtcgcg cgtccccggg gtgccgctgg
     8341 cgccgaatca gatcgaggaa gttctgcacg accgtctggc cgccagccga aggggggcgc
     8401 aatgttccgg ctgaaggaag attgggagct cgagccgaaa ccgcgttgcc tggtgctcga
     8461 ggactacgtc ggcgcgaccc cgcgttggtg tcccggttgc ggcgatcatg cggtgctcgg
     8521 cgcgctccag cggctggccc gcgacgagca gctgcccccg gagcagaccg tcgtcgtctc
     8581 cggtatcggc tgttccagtc ggttcccgca ctacatgaag acctacggct tccacggtct
     8641 acacgggcga ccgctccccg tcgcgtgcgg catccgtgcg cgccgcccgg accttcacgt
     8701 cttcgtcgtg accggcgacg gcgactgctg cgcgatcggc acggcgcact ggatccacgc
     8761 cgtccgctac aacatgcgga tgacggtgat gctgctcgac aaccacgtct acgggttgac
     8821 caaggcccag agctcaccga cctcgcggct ggggcagaag tcgaacacgc atccgttcgg
     8881 tgccccgttg cgtccgctca atccgctgac ggtcaccctg ggcatcacca acgcgtcgtt
     8941 cgtcgcacaa acgctcgatt ggaacccgcc gcacctgtac gccaccctgg ccgaggcgca
     9001 ccgtcatcgc gggctgtcgt tcgtgcgggt actgcaacgg tgtccgcact acctggaagg
     9061 tgcgtgggac ggcgtgcagc gcgacccgac ccgcgtcctg ctgctcacac acgccaacgg
     9121 gattccgttg gacgagtcgc tcgctcggcc gttcacgaac cgcgtcgagc acgacccgtc
     9181 cgacttggcc cgcggccgcg agttggcggc gcgcgaggac gtcgtgccga tcgggctgtt
     9241 tttccgcgac gaggacgccg agcggtacga cgagctgtcg gcggccggtc tcggcatgag
     9301 tacggcggag aaactaggaa ggatcgaggc ggagctggag cgtttcgtca tctgaggtgg
     9361 atcggcatcg atgaagacga cggacagcga gaggaccggt gagacagcgg ctcgagcgcg
     9421 gcaggaatcc gccgccgccc gcgacggggc gcgcatcttc gcctcgacct ccgccgcgat
     9481 cgatcgcacc gagcgcctgc tgtgcggcga cggcgtggtc aggtcggttg ccgtcgccga
     9541 ggggctggcg ctcgccggtt cgcacgccgc gacgagtctc ggtgcgggcg cgccccggcc
     9601 ggggccgtcg gtcccgcgca gcgaagcggc ctgggttcat caccgcgtgg ccggccccgg
     9661 cacggcgctt cgcggagcgg cggcgttcga gctgttggcc gccgacgccg gcgaggcggt
     9721 cgcgcacacg ctggccgcgc ggcggatggt acgcctgctc ggtcgcccgg ggatctgctc
     9781 gatcgaggcc gccgcggcgg accgtccgtg ccacgtgcgg ctgccggacg cgcgggaggt
     9841 ctcggccgtg ctcgacgatc gatcgacgtg tgccggcgga gtctcggccg ccgccgaccg
     9901 ggtgctcggc cggcgcggga tcctgccgac ctatcgggtc gacgacgcga cgctgttgtt
     9961 ggtcgcggcg ggcgaggatc gcctgcgggc gcatcggctg gtcgatcgtc tgcgtcgccg
    10021 cggcttgacc tgcggcgtcg tcggcgtcgc gatggtccat ccgttcccgg cggcggagct
    10081 acgagcggcg ctcgcgggct gcgagtcggt gtgggtcgtc acaccggcag tcggcgagga
    10141 tgccgcggaa gccgcgcgcg cagccgggtg cgccgcgatc gtcgatcacc gcagcgcggg
    10201 cggcgaactg cttttgccgc ccgaactcgc cgagcggttt ccggcgaacg ccgggcgtgc
    10261 tgccgtcgcg acgcacgagg ccgacgaccg gtcggcggtc gcgctcgccg tcgatccgcc
    10321 cgaccccgag gcgctccgcg ccgcggtcgc ggcggcgtcc gaggccggct gggccgagcc
    10381 gtggaacgtg atccggctct cgccgggcgc cgatccggcc ggcgtcgtgt tgggtcgtga
    10441 ttcgacctgc gcgccgcccg cgcggatcga cgtcggcgtc atcgtccggc cggcggcgga
    10501 cgacggacgc agggccggcg cgttattgtc ccgtctcgat cggcggagca cgttgctgct
    10561 cgtcaccgac gacgaccgcc cgctcgacac ctggcgagcg ctggggcccg cgcgtcgggc
    10621 cgcgatcacc ggcgggcgtc ccgcgacgcg ctggatctcg atggcggatt ggtcgaccgt
    10681 ctccgccgat ctcgcgcggt tgatccgcga gcggttcgcc ggggcgcgat cgcagcccgc
    10741 ggacggtacg ctccggtcga tcccgatcga cgtcgagacg gtcgaggcgg agctgcgcgc
    10801 cgaagccgcg accgtcggcg gggaggcggc atcaccggtg atgcccgagg tcgcgctggt
    10861 cgagcgatcc tcggtcgagt cgtgggagtt ccaccgcacg gcaccgcggt gcttcgacga
    10921 acgaccgctc ggcggtccgc tcgtgccctg ggcggcggtc gggctcggcg ccgccacccc
    10981 cgagtggcga gcctacccgt tcgtgctcga cgcccgcggg gtcggcgccg gtcacgcccc
    11041 gctggacgtg ctgctgcaag aggcgatcga acacgccggc gacggcctgt ccagcggaat
    11101 tctctcgcgc cgacgtccga cggtcctgcg cctcgccaac cgcacggcat cggcgagtgg
    11161 gtcgctcgcg cctccggcgg agttggtgcg cgccgcgttg tcggcgctgc cggacgaact
    11221 cgatctgagc gaggccgggc gcgccgagct gcagcgcgag atcgcgaccg tcgaaccgcg
    11281 gatgccggcc ggcggacggg cagtcgggtt cgacgagcga acgctcgaca tcctgctggc
    11341 cgagacgctg gtcgcacgcc gggcgccggc gcgagccgag tttcgccgtt gcgtcgagca
    11401 actcgtcgag cgtctcgacg agttgctgcg gatcgacgac ggccatcggc cgcaggcggc
    11461 gagccccgcg gcgattgccc cgacgctcgg cgccggggag cgcttcgtcg atcccgagcg
    11521 gctgtcgcgg cacctgcccg cctatcgcgg ttctgcgccg ctcgagcccg agcgccgcgc
    11581 gcggatcgag cggtcgcgcg ccacgcttcg ctcgtggctc gacgcgggag gctcgagcgc
    11641 ggacctgatc ttggtgcaga cgacggcgct cggtccggat ccgcggccgc gatgtcgcgt
    11701 cgtcgtccac ccggatccgc tgcgcgccgc cgtcggcttg tgggacgggc tggcccgctc
    11761 gatggtcgaa ccggtccgcg cgctgcgcac cgcacggctc gacgtgtcgg ggcactatga
    11821 gcccgagcgg cacgatcgtc cgatcgccgc cctcgacccg ctcgggttca gcgcggagga
    11881 catgctgcag attcccgccg tcgtcgtccg cgacgacgag cggcccctcg gcgaggacgc
    11941 actcggggcg ttctccgccc tgctccggtc cgggcgaccg gtccacgtgc tcgcgacgcg
    12001 acgggcgacc gacgtttcgc tgtcgcgcga caccggcccg ggttacgatc cgggcaccgg
    12061 ttacctcgcg ctcgcgcatc gggaggcgtt cgtcatccaa acgagcctca cgcggccggc
    12121 cacgttgacc gaagcgttcg agcgcatggc gatcggtctc cggccgtcgg tggcgctcgt
    12181 caccgagccg gactgggacg ggccgatcga gccttggcgg cagctcgtgc tggccgagtt
    12241 cggccgcgac ccgtatcact tccgttacga cccggcggcc ggtcccagct gggccgagcg
    12301 cttcgacatc gacgacaacc ctcagcccgc ggccggttgg ccgcgccaca gcgtcgagca
    12361 ggccggcacc gcccgcggcg cgtcggtcgt cgagccatgc ttcacgatgg ccgatctcgc
    12421 cgcggcggtc ccgcggtcgc gcggtcattt caggacgctg tcggcgaacg agtgggccag
    12481 cgatcagacc gagatcggtt cctattgcga gctcgagccg accgagcagc tccgg
//
LOCUS       DASPGN010000342         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_528850, whole genome shotgun sequence.
ACCESSION   DASPGN010000342 DASPGN010000000
VERSION     DASPGN010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528850"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VD788_09830"
     CDS             complement(<1..952)
                     /locus_tag="VD788_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HXV76608.1"
                     /translation="MAEIGINHNGSLDVARELVDAAARAGVEVVKHQSHVIADEMTAA
                     ARAVIPGNADRSIYEIMAQCALSPDDERELKRYVESRGMIFLSTPFSRAAADNLQRMR
                     VEGYKIGSGECNNYPLIDHVARFGKPVILSTGMNSIESVGPAVEILRAHRVPFALLHC
                     TNVYPTPARLVRLGAMAELKAAFPDAVVGLSDHTQGNYTCFAAVALGAAIVERHFTDR
                     MDREGPDVVCSADERAMAELIEGSRQIFLARGGHKTPVEEERVTMEFAFATVVAIRPI
                     ARGETLSRENIWVKRPGTGEIGAMHFERLLGRTAARDVPCD"
     gene            complement(1046..1121)
                     /locus_tag="VD788_09835"
     tRNA            complement(1046..1121)
                     /locus_tag="VD788_09835"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1086..1088),aa:Ala,seq:ggc)
     gene            1221..1319
                     /locus_tag="VD788_09840"
     tRNA            1221..1319
                     /locus_tag="VD788_09840"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1255..1257,aa:Sec,seq:tca)
     gene            1363..2001
                     /locus_tag="VD788_09845"
     CDS             1363..2001
                     /locus_tag="VD788_09845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HXV76609.1"
                     /translation="MHRRATRIAEYLLLFIALPVYLARRPAPIPVFPLLWLLAAVCLL
                     YLVTRAEFDRRRLWDARYLSARLRAASLPFVVLAPPLAAAAWLVEPERFFGLVRFRPG
                     LWTLVMVLYPILSVYPQGVVYRAFVFERYRGVFGGPVGSVLASAVAFGAAHLVFRNWV
                     APTLGLGGGLLFAWTYQRTGSSLVAAIQHAAFGCWIFTTGLGVYFYYGAVRF"
     gene            complement(1980..>2359)
                     /locus_tag="VD788_09850"
     CDS             complement(1980..>2359)
                     /locus_tag="VD788_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76610.1"
                     /translation="FEVVELFLAGPGATCLELELGPHGHYLALLLSGPRRPLDRVIAL
                     APRIERAGRQWRAEVELPSAIVPRPIVAWNAYAMHGAGARRRFLAVAPVPGDRPDFHR
                     PDRFVPGDPTADEQGPDQNRTAP"
BASE COUNT          309 a          820 c          816 g          414 t
ORIGIN      
        1 ggtcgcacgg cacgtcccgc gccgccgtcc gaccgagcaa gcgttcgaag tgcatcgcgc
       61 cgatctcgcc ggtgccgggc cgtttgaccc agatgttctc gcgcgacagc gtctcgccgc
      121 gggcaatcgg tcgaatcgcg acgaccgtgg cgaaggcgaa ctccatcgtg acgcgctcct
      181 cctcgaccgg cgtcttgtgc ccgccgcgcg cgaggaagat ctggcgcgat ccctcgatca
      241 gctcggccat cgcccgctcg tcggcggaac agacgacgtc cggtccctcg cggtccatcc
      301 gatcggtgaa gtgccgctcg acgatcgccg cacccaacgc caccgcggcg aaacacgtgt
      361 agtttccctg cgtgtgatcg gacagcccga cgacggcgtc ggggaacgcc gccttgagct
      421 cggccatcgc acccagccgc accagcctcg ccggcgtcgg atagacgttc gtgcagtgca
      481 gcagcgcgaa cggcacgcgg tgcgcgcgca ggatctcgac cgcggggccg accgactcga
      541 tcgaattcat cccggtgctg aggatcaccg gcttgccgaa gcgcgcgacg tggtcgatca
      601 acgggtagtt gttgcactcg ccggagccga tcttgtatcc ctcgacccgc atccgctgca
      661 ggttgtcggc ggcggctcgc gagaacggcg tgctcaggaa gatcatccct cgactttcga
      721 cgtagcgctt gagctcccgt tcgtcgtcgg gcgacaaagc gcattgggcc atgatctcgt
      781 agatcgagcg gtcggcgttg ccggggatca cggcgcgcgc ggcggccgtc atctcgtccg
      841 cgatcacgtg ggattgatgc ttgacgacct cgacgccggc gcgcgcggcg gcatcgacca
      901 gctcgcgggc cacgtcgagc gatccgttgt ggttgatacc gatctcggcc acgacgagcg
      961 gcggctcgtc atctccgacc gtccgtcctg cgatgtgaaa ctcggccacg gcgtcgactc
     1021 tgccggctca atctcgttcg gtcgttggtg gagctgagga ggatcgaact cctgacctcc
     1081 tgaatgccat tcaggcgctc tcccagctga gctacagccc cacgacggaa agtggcgccc
     1141 acgatagcat aggccgagag cgcggtccgg cggccggttg acgctatccg ggccccggcc
     1201 tagcatgcgt ggtcccgtcg ggaagcgcat ggggcccggt ggcccccccg gtcttcaaaa
     1261 ccgcgagtcg ggcggcctcc gccgtccggg gtgggttcga ctcccacacg cttccgccag
     1321 ccgctcgcgc gcggtgccga gcgatcgtcg taagatggct cgatgcatcg ccgcgcgacg
     1381 cggatcgcgg agtacctgct gctgttcatc gcgctcccgg tctacctcgc gcgccgtccg
     1441 gcgccgatcc cggtttttcc gctgctgtgg ctgctcgctg cggtctgcct gctctacctc
     1501 gtgacccgcg cggaattcga tcgccgccgg ttgtgggacg cacggtacct gtcggcgcgg
     1561 ctgcgcgccg cgtcgctccc gttcgtcgtg ctcgcgccgc cgttggccgc ggcggcctgg
     1621 ctcgtcgagc cggagcggtt cttcggtctg gtccgctttc gtccggggtt gtggacactg
     1681 gtgatggtgc tctatccgat cctgtcggtc tacccgcagg gcgtcgtcta tcgcgccttc
     1741 gtcttcgaac ggtaccgggg tgtgttcggc ggtccggtcg gatcggtcct ggcgagcgcc
     1801 gtcgcgttcg gcgcggcgca tctcgttttc aggaactggg tcgcgccgac tctcggcctt
     1861 ggcggtggcc tgctgttcgc ctggacctat cagcgcacgg gctcgtcgct ggtggcggcg
     1921 atccagcacg ccgcgttcgg ctgctggatc ttcaccaccg gcctcggggt ctacttctat
     1981 tacggcgccg ttcgattctg atccggtccc tgctcgtcgg ccgtcggatc gcccggcacg
     2041 aagcggtcgg gccggtggaa gtcgggccga tcccccggta ccggggcgac cgcgaggaag
     2101 cggcggcggg cgccggcgcc gtgcatcgca taggcgttcc acgcgacgat cggtcgcggg
     2161 acgatcgccg acggcagctc gacctcggcg cgccactgtc gtccggcgcg ctcgatccga
     2221 ggagcgagcg cgatgacccg gtcgagcgga cggcgggggc cggagagcag cagcgccagg
     2281 tagtggccgt gcgggccgag ctcgagctcg agacaggtcg cgccggggcc ggcgaggaac
     2341 agctcgacga cctcgaact
//
LOCUS       DASPGN010000343         7649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_296369, whole genome shotgun sequence.
ACCESSION   DASPGN010000343 DASPGN010000000
VERSION     DASPGN010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7649
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296369"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /gene="hemG"
                     /locus_tag="VD788_09855"
     CDS             complement(<1..661)
                     /gene="hemG"
                     /locus_tag="VD788_09855"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HXV76611.1"
                     /translation="MQRRTVAVIGGGISGLTVAYELLERSQRLPEGTGIVCLEAGDRA
                     GGNIRSERIDGYTCEAGPTGFLDSAPATVTLARRLGLVERIVRAKPAAAHRYVFRKGA
                     LRKVPLDPFSFLTSGVLSPWGKLRVLGEPFAPRGRNDDESVHEFVSRRIGAEAAAVLV
                     DAMVSGIYVGDARRLSLPATFPKMRQMEREHGGLFRSMWARRRRARADGDAATAGGPA
                     GP"
     gene            complement(688..1719)
                     /gene="hemE"
                     /locus_tag="VD788_09860"
     CDS             complement(688..1719)
                     /gene="hemE"
                     /locus_tag="VD788_09860"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HXV76612.1"
                     /translation="MTDALFLRACRGEKTERPPLWIMRQAGRYLPEYRELRRSTDFLT
                     LCRTPELAARVTLQPIDRFGFDAAILFSDILVPAAALGVRFDFNPGPVLDDPIRTPAQ
                     IDALRVGEPESAVPYVFETVRILRRELADRVPLIGFAAAPLTFAVYLVEGGGSKNFDR
                     IKRLLFGDPRAAHGLLEKVAELTERYLRAQIQAGAQAVQLFDTWAGLFDPACYREFGL
                     RYARRVLDGLAPAGVPRIYFALDASHLLEQVGESGADVIGADWRVPLDVVSDRLGRRF
                     PLQGNLDPCVLLTTPQIVGRRVGQLLDGARDLPGHIFNLGHGVLPATPVENVEALVEA
                     VRAAGRARG"
     gene            complement(1716..2882)
                     /locus_tag="VD788_09865"
     CDS             complement(1716..2882)
                     /locus_tag="VD788_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXV76613.1"
                     /translation="MSSDTPAPRPLTQLTDEETMFRDAIREFAEGEIAPRTREMERDG
                     RYDDALLRSLFEIGAMAIEVPQEYGGVGGSFFQAVLAVEEISRADASVGVLVDVQNTL
                     FIVALTRHGTEQQKRRYLPRLARDTVAAYALSEAGSGSDAFALACRAERRDGGWVLDG
                     HKLWITSGAEAGVYVVFANADPAQGYRGITAFLVERDDPGFSVGKRESKLGLRASSTV
                     ELLFDGCRIPADRVLGQVGRGYKYAIETLNEGRIGIGAQMVGVSRAALDAATTYSKER
                     KQFGKPISEFQAVQHQLAQCATRLEAARLLVYNAARLRDAGLPFIAEAAMAKLYSSEA
                     AEFITSAAVEIFGGYGYSTEYPVEKYYRDAKIGKIYEGTSFMQLNTIAKLLLGA"
     gene            3036..3536
                     /gene="tpx"
                     /locus_tag="VD788_09870"
     CDS             3036..3536
                     /gene="tpx"
                     /locus_tag="VD788_09870"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HXV76614.1"
                     /translation="MAKITLKGNPFQTVGELPAVGAKAPRFHLVDADMNDVGLEEFAG
                     KRKILSINPSFDTPVCATSARKFNDRASSLDNTVVLAISADLPFAQKRFCASEGLNDV
                     VTLSMMRSKSFAKDYGVLIEDGPLAGITARALLVLDENDRVLYSQLVGEIAEEPNYEA
                     ALSKLD"
     gene            complement(3520..4359)
                     /locus_tag="VD788_09875"
     CDS             complement(3520..4359)
                     /locus_tag="VD788_09875"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXV76615.1"
                     /translation="MLVAGCGWLGRAVGRELSRRGDRVIGIRRDPAAGTELERDGIRP
                     LIVDLATVSAARSLPGGVDAVIACPAPADPGVEAYRRSYVDAVATLVDRYAAELDRAF
                     VYTGSTGVFGQRDGGQVDERSPTLPTSPTAGVLVEAEQLVLASGVGSIVRLSGLYGPS
                     RYGVIDRVRNGRLALGPADGRFMNFCHLDDAVRSVVAAIDRGRPGTVYHASDAEPTRR
                     REVIRWIAARAGFDPTVGTGDDAAEGPDRRVSAVWSREALGVELAYPSFRDGLADAIS
                     RAC"
     gene            4442..5431
                     /locus_tag="VD788_09880"
     CDS             4442..5431
                     /locus_tag="VD788_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV76616.1"
                     /translation="MPDAIVASGLEKTFVRPRSIREIVTRPFGRARRIDALRGVDLTV
                     AEGEILGLLGPNGAGKTTLLKILACLVLPDAGRAVVGGSDTVDENRVKRRIGLVHSDE
                     RSFYWRLSARENLRFFARLYDVPRRVTESRIAMLLERVDLAEAADRPFSGYSSGMKQR
                     VSIARALLHDPPILLMDEPTRSLDPTSSKQLRTFIAEELNRHEGKTIVLATHDLREAE
                     ALSHRVAILARGRVRQIGTVDEVRRWGLSERLFRLELDPFDGAIGGTFRVVDDQTADG
                     VRRVTVALPADRSLDELLKKLVGLGVGVHLCDRIEPDLEQAFARILEAESAEP"
     gene            5428..6234
                     /locus_tag="VD788_09885"
     CDS             5428..6234
                     /locus_tag="VD788_09885"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV76617.1"
                     /translation="MIDNLLKLWAFIKRDFLSEVSYRLAFLMSVAGMFFSLLAFYFLT
                     KMIDPATAGLDGIPPFDWLLIGLSFQFYFSTALYSFSARVRNEQLLGTLEAMLVTPTP
                     TSMVVFSSAAWDFVYGGIRVLLYLLFATLVFGVKLHLQSPMSLIIGVLLTLLSSAGLG
                     ILSASFILYFKRGDPINFLLSGATTLLGSVFFPVQLLPGWVQPLSEYLPITWSLRIVR
                     GSLLQGRTFGELQRELIVLALLTVVLLPGGLLCSRFAIRRAKREGTLIQY"
     gene            6341..7576
                     /gene="icd"
                     /locus_tag="VD788_09890"
     CDS             6341..7576
                     /gene="icd"
                     /locus_tag="VD788_09890"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HXV76618.1"
                     /translation="MSKAIEGEPIRLLNGRLRVPDRPVIPYVEGDGIGPDIWAATRTV
                     LDAAVAKAYEGKKRIAWYEIFAGEKANEAYGEWLPEASVQAVREYKVAIKGPLTTPVG
                     GGIRSLNVALRHLLDLYACVRPVRHIPGVPSPVKRPDEIDVVIFRENTEDVYAGIEWK
                     SGSPEARKLIEFIGSELGKSIRPDSGIGIKPISMFGTSRLVGKAIRYAIDHGRESVTL
                     MHKGNIMKFTEGAFKDWGYEIAASQFADRTLSEELLWSEHDGRRPDGRVVIKDRIADS
                     MFQQILLRPAEYSVLATPNLNGDYLSDACAAQVGGLGMAPGANMSDTIAFFEATHGTA
                     PKYANLDRVNPGSLILSGVMLLQHLGWNEAAERVKTGLARAVERKTVTYDLARQMEGA
                     REVSCSEFAEAIVSASVSA"
BASE COUNT         1217 a         2663 c         2596 g         1173 t
ORIGIN      
        1 cgggccccgc cgggccgccg gccgtggcgg cgtcgccgtc ggcccgcgct cgccggcgtc
       61 gcgcccacat cgagcggaac aggccgccgt gctcgcgctc catctgtcgc atcttgggga
      121 aggtcgccgg cagcgagagg cgccgcgcgt cgccgacgta gatcccgctg accatcgcgt
      181 cgacgagcac cgcggccgcc tcggcaccga tccggcgcga gacgaactcg tggaccgact
      241 cgtcgtcgtt ccgaccgcga ggagcgaacg gctcgcccag cacgcggagc ttgccccagg
      301 gtgagagaac acccgaggta aggaacgaga acgggtcgag cgggaccttc cgtaacgccc
      361 ccttgcgaaa gacgtaacgg tgggcggccg cgggtttggc ccgcacgatc cgctcgacga
      421 ggccgaggcg gcgcgccagc gtcaccgtcg ccggggcgct gtcgaggaag ccggtcggac
      481 cggcttcgca ggtgtagccg tcgatccgct cgctgcggat gttcccgccc gcccgatcgc
      541 cggcctcgag gcagacgatc ccggtaccct cgggcagacg ttgggaacgt tcgagcagct
      601 cgtatgcgac ggtcaggccg gagatccctc cgccgatcac ggccaccgtc cgacgttgca
      661 tcacccgccc ccgacgcgac ccgggtctca gccccgggcg cgacccgccg ctcgaaccgc
      721 ctcgaccagc gcctcgacgt tctcgaccgg cgtcgccggc agcacgccgt ggcccagatt
      781 gaagatgtga cccggtagat cgcgcgcccc gtcgagcagc tggccgacgc gccggccgac
      841 gatctgaggc gtggtgagca acacgcacgg atcgaggttc ccctgcagcg ggaaccgccg
      901 cccgagccgg tccgagacca cgtcgagcgg cacccgccag tcggcgccga tcacgtcggc
      961 ccccgattca cccacctgtt cgagcaggtg gctcgcatcc aatgcgaaat agatccgcgg
     1021 aacgccggcg ggcgccagtc cgtccagcac gcgccgcgcg tagcgcaggc cgaactcgcg
     1081 gtagcacgcc gggtcgaaca acccggccca ggtatcgaac agctggaccg cctgcgcgcc
     1141 ggcctggatc tgcgcccgca aatagcgctc ggtcagctcc gcgacctttt cgagcaaacc
     1201 gtgcgccgca cgcgggtcgc cgaacagcag ccgcttgatc cggtcgaagt tcttcgagcc
     1261 gccgccttcg acgagataga cggcgaaggt caacggcgcg gcggcgaagc cgatcagcgg
     1321 gacgcggtcg gccagctcgc ggcggagaat gcgtacggtt tcgaagacat agggcaccgc
     1381 cgactccggc tcgccgacac gcagggcgtc gatctgcgcc ggcgtccgga tcgggtcgtc
     1441 gagcaccgga ccgggattga agtcgaagcg gacgccgagc gccgccgccg ggacgaggat
     1501 gtccgaaaac aggatcgccg cgtcgaagcc gaagcggtcg atcggctgca acgtgacccg
     1561 cgcggcgagc tccggcgtcc ggcacaacgt caagaagtcg gtcgagcggc gaagctcgcg
     1621 gtactccggc aggtagcggc ccgcctggcg catgatccac aacggcgggc gctccgtctt
     1681 ctcgccgcgg caggcccgca agaacagcgc gtccgtcatg cgccgagcag gagcttggcg
     1741 atcgtgttga gctgcatgaa cgacgtgcct tcgtagatct tgccgatctt ggcgtcgcgg
     1801 tagtacttct cgacggggta ctccgtcgag tagccgtaac cgccgaagat ctcgacggcg
     1861 gccgaggtga tgaactcggc ggcctcgctc gagtagagct tggccatcgc cgcctcggcg
     1921 atgaacggca ggccggcatc gcgcaatcgg gccgcgttgt agaccaacag ccgcgccgct
     1981 tcgagccgtg tcgcgcactg ggcgagctgg tgctggactg cctggaactc ggagatcggc
     2041 ttgccgaact gcttgcgctc cttgctgtag gtcgtcgcgg catcgagcgc cgcgcgggag
     2101 acgccgacca tctgcgcgcc gatgccgatg cggccctcgt tcaaggtctc gatcgcgtac
     2161 ttgtatccgc gcccgacctg gcccagcacg cggtcggccg ggatcctgca cccgtcgaac
     2221 agcagctcga cggtgctcga cgcgcgcagc ccgagcttgc tctcgcgctt gccgacgctg
     2281 aaccccgggt cgtcgcgctc gaccaggaag gcggtgatcc cacggtagcc ctgagccggg
     2341 tcggcgttgg cgaacacgac gtagaccccg gcctcggcgc cgctcgtgat ccacagcttg
     2401 tgcccgtcga gcacccagcc cccgtcgcgc cgttcggcgc ggcaggcgag cgcgaaggcg
     2461 tccgagcccg agccggcttc ggacaacgcg taggccgcga ccgtgtcacg ggccagccgc
     2521 ggcaggtagc gccgcttctg ctgttcggtg ccgtgccgcg tcagtgcgac gatgaacagc
     2581 gtgttctgca cgtcgaccaa cacgccgacc gaggcgtccg cgcgggagat ctcctcgacg
     2641 gcgagcaccg cctgaaagaa gctgcccccg acgccgccgt actcctgcgg cacctcgatc
     2701 gccatcgcgc cgatctcgaa cagactccgc agcagcgcgt cgtcgtagcg tccgtcgcgc
     2761 tccatctccc gcgtccgggg cgcgatctcg ccctcggcga actcgcggat cgcgtcgcgg
     2821 aacatcgtct cttcgtcggt cagctgggtc aacggccgtg gtgcgggggt gtcgctggac
     2881 atgtcgtgct cccggtcctg gtcgagggaa gcccattatg acgaatgccg gcggggcgac
     2941 caagccccgg cggccgtcag atcggccacc gccgccgtgc taacctgggg gcgacccccg
     3001 tggatcgtca tccgtcgatg aaagcgaggt aacgcatggc caagatcacg ctcaagggca
     3061 acccgttcca gacggtcggc gagcttcccg cggtcggcgc gaaagccccg cggttccacc
     3121 tggtcgacgc cgacatgaac gacgtcggtc tggaggaatt cgccggaaaa cgcaagatcc
     3181 tctcgatcaa cccgagcttc gacacgccgg tctgtgcgac ctccgcgcgc aagttcaacg
     3241 accgggcctc gtcgctcgac aacacggtcg tgctggcgat cagcgccgat ctaccgttcg
     3301 cgcagaagcg cttttgcgcg agcgagggcc tgaacgacgt cgtcacgctc tcgatgatgc
     3361 gttcgaagag cttcgccaag gactacggcg tgctgatcga ggacggaccg ctcgccggca
     3421 tcaccgcccg cgcgttgctc gtgctcgacg agaacgaccg cgtgctctac tcccagctgg
     3481 tcggcgagat cgccgaagag ccgaactacg aggccgcgct cagcaagctc gactgatcgc
     3541 gtcggccaga ccgtcgcgga aggagggata ggccagctcg acgccgagcg cctcgcgcga
     3601 ccagacggcc gagacgcgtc ggtcgggacc ctccgccgcg tcgtcgccgg taccgacggt
     3661 cggatcgaaa ccggcgcgcg ccgcgatcca gcggatcacc tcccggcgcc gggtcggttc
     3721 cgcgtcgctc gcgtggtaga ccgtgcccgg ccggccgcga tcgatcgccg ccacgaccga
     3781 cctgaccgcg tcgtcgaggt ggcagaaatt catgaagcga ccgtcggccg gaccgagcgc
     3841 cagccgcccg ttccgcacgc gatcgatcac gccgtagcgc gacggcccgt agagccccga
     3901 cagccgaacg atcgacccga cgccgctcgc cagcaccagc tgctcggcct cgaccagcac
     3961 gccggccgtc ggcgaagtcg gcagcgtcgg gctgcgctcg tcgacctgac cgccgtcgcg
     4021 ctggccgaag acgccggtcg agccggtgta gacgaacgcg cggtcgagct cggccgcgta
     4081 acgatcgacc agcgtggcga cggcgtcgac gtagctgcgc cggtaggcct cgaccccggg
     4141 gtcggccggc gccgggcagg cgatgaccgc gtcgactccc cccggtagcg accgcgccgc
     4201 cgaaacggtg gccaagtcga cgatcagtgg gcggatcccg tcgcgctcga gctcggtgcc
     4261 ggccgccggg tcgcggcgga tgccgatcac gcgatcgccg cggcgagaca actcgcgccc
     4321 gacggcccgg ccgagccaac cacatcccgc gacgagcacc cgcatcgccg gcaaaggata
     4381 gcgcggattc gccgcgggaa gcctgctatc ctcgcgtggt ccgcgccccg gagcacaggt
     4441 tttgcccgac gccatcgtcg ccagcggtct agagaagacc ttcgtccggc cgcgctcgat
     4501 ccgcgagatc gtgacgcgtc cgttcgggcg cgcgcgcagg atcgacgcgc tgcgcggcgt
     4561 cgacctgacc gtcgccgagg gcgagattct cggcctgctc gggccgaacg gcgcgggcaa
     4621 gaccacgctg ctcaagatcc tggcgtgcct ggtgctgccc gacgccgggc gcgccgtcgt
     4681 cggcggcagc gacacggtcg acgagaaccg ggtcaagcga cgaatcggtc tggtccactc
     4741 cgacgaacgg tcgttctact ggcggctgtc ggcgcgcgaa aacctgcgct tcttcgcccg
     4801 gctgtacgac gtaccgcgac gggtgaccga gagccggatc gcgatgctgc tcgagcgggt
     4861 cgatctggcc gaggccgcgg accgcccgtt ctccggttac tcctccggaa tgaagcagcg
     4921 ggtgtcgatc gcgcgggcgc tgctccacga cccgccgatc ctgttgatgg acgagccgac
     4981 ccgcagcctc gatccgacct cctcgaagca gctgcgaacg ttcatcgccg aggaactgaa
     5041 ccgtcacgag gggaagacga tcgtattggc gacccacgac ctgcgcgaag ccgaggcgct
     5101 gtcgcaccgg gtggcgatcc tcgcccgtgg acgggtgcgg cagatcggca cggtcgacga
     5161 ggtccgtcgc tggggcctca gcgaacggct gttccgtctc gagctcgatc cgttcgatgg
     5221 ggcgatcggt ggaacgttcc gcgtcgtcga cgaccaaacc gccgacggcg tacgccgggt
     5281 gaccgtcgcc ctcccggccg accggtcgct cgacgagctg ttgaagaagc tcgtcggtct
     5341 cggcgtcggc gtccatctgt gcgaccggat cgagcccgac ctcgagcagg cgttcgcgcg
     5401 gattctcgaa gcggagtccg ccgaaccgtg atcgacaatc tgctcaagct gtgggcgttc
     5461 atcaaacgcg acttcctgtc ggaagtttcg taccgcctgg cgttcctgat gagcgtcgcc
     5521 gggatgttct tctcgttgct ggccttctac ttcctgacca agatgatcga cccggcgacg
     5581 gcgggcttgg acgggatccc gccgttcgac tggctgctga tcggcctgtc gttccagttc
     5641 tacttttcga ccgcgctcta ttcgttctcg gcgcgggtgc gcaacgagca gctgctcggc
     5701 acgctcgagg cgatgctggt cacgccgacg ccgacttcga tggtggtctt ttcgtccgcc
     5761 gcctgggact tcgtctacgg cggaatccgc gtgctgctct acctgctgtt cgcgacgctc
     5821 gtcttcggcg tcaagctgca cctgcaaagc ccgatgtcgc tcatcatcgg cgtgctgctg
     5881 acgctgctct catcggccgg tctcggcatc ctgtcggcgt cgttcatcct ctacttcaag
     5941 cggggcgacc cgatcaactt cctgctcagc ggcgcgacca cgctgctcgg ctcggtcttc
     6001 ttcccggtcc agctgctgcc gggttgggtg cagccgctgt cggagtacct accgatcacc
     6061 tggtcgctgc ggatcgtccg cggctcgctg ctgcaggggc gcaccttcgg cgagctgcag
     6121 cgcgagctca tcgtgctggc gctgttgacc gtggtcctgc tacccggcgg gctgctctgc
     6181 tcgcgcttcg ccattcgccg ggccaagcgc gaggggacgc tgatccagta ctgaatcggt
     6241 acgccgaacg gtttgacacc ctctccgccg ggttgtctaa tgctcctttc cgaactcggc
     6301 aatctcccga tcgaaggagt ccgtcgtgac gccggcaagc ttgtccaaag caatcgaagg
     6361 cgaaccgatc cgtctgctga acggccggct tcgcgtaccc gaccgtccgg tgatccccta
     6421 cgtcgagggc gacgggatcg gtcccgacat ctgggccgcc acgcggacgg tgctcgacgc
     6481 cgcggtcgcc aaggcctacg aggggaagaa gcggatcgcc tggtacgaga tcttcgccgg
     6541 cgagaaagcc aacgaagcgt atggcgagtg gctgcccgag gcgagcgtgc aagcggtgcg
     6601 cgaatacaag gtcgcgatca aggggccgct gacgacgccg gtcggcgggg gcatccgcag
     6661 tctcaacgtc gcgctacgac acctgctcga tctctacgcc tgtgtgcgcc cggtgcgtca
     6721 cattccgggc gtgccgtccc cggtcaagcg gccggacgag atcgacgtgg tcatcttccg
     6781 cgagaacacc gaagacgtct atgccggtat cgagtggaag tcgggaagtc ccgaggcgcg
     6841 caagctgatc gagttcatcg gtagcgagct cggcaagagc atccgtcccg actccggcat
     6901 cggcatcaag ccgatctcga tgttcggcac gtcgcgtctg gtcggtaaag cgattcgcta
     6961 cgcgatcgac cacgggcgag agagcgtcac gttgatgcac aagggcaaca tcatgaagtt
     7021 caccgaaggg gcgttcaagg actggggcta cgagatcgcg gcgagccagt tcgccgatcg
     7081 cacgctgtcg gaggagctgc tgtggtcgga gcacgacggc cggcgtcccg acggacgcgt
     7141 cgtcatcaaa gaccgcatcg ccgactcgat gttccagcag atcctgctcc gtccggccga
     7201 gtactccgtc ctggccacgc ccaacctgaa cggcgactac ctctcggatg cgtgcgccgc
     7261 acaggtgggg ggcctgggca tggcgcccgg cgccaacatg tcggacacca tcgccttctt
     7321 cgaggccacg cacgggacgg cgcccaagta cgcgaacctg gaccgggtca accccggctc
     7381 gctgatcctc tccggggtca tgctcctcca gcacctgggg tggaacgagg cggccgagcg
     7441 ggtgaagacg ggcctggcgc gtgcggtgga gcgcaagacc gtgacctacg atctggcgcg
     7501 gcagatggaa ggggcgcggg aggtttcctg ctcggagttc gcggaggcga tcgtgagcgc
     7561 ctcggtatcg gcctgaggga catagaagat gcgcaacaag atcacggtga tcggcggcgg
     7621 gttcgtgggc gagcacgtgg ccgtgggct
//
LOCUS       DASPGN010000344         4896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_177694, whole genome shotgun sequence.
ACCESSION   DASPGN010000344 DASPGN010000000
VERSION     DASPGN010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177694"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VD788_09895"
     CDS             complement(<1..769)
                     /locus_tag="VD788_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76619.1"
                     /translation="MSIRSMLVWLAIVTGMPSWAADPEATCPTRNEVSTTLRLVDVRR
                     GSRTARFGADPPFALYDKAAANVGRPVASRDGKTMRAVLVIDRPVETIWKALNDEPHH
                     ALDGKYLPLRHSEVIGGTPRGIERTLFQYFKKAGIGRWWVSRVEMNGELYRDSAGRIW
                     ELSWRGVSDQVDAAQPPISMIADELPPLEETYGAWFLVPLDPRCTLIEYFNHTEPGGF
                     VSIAQNLLAKSSLRETLQAIVRLADEHLPEADPAAVFL"
     gene            complement(814..2598)
                     /locus_tag="VD788_09900"
     CDS             complement(814..2598)
                     /locus_tag="VD788_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76620.1"
                     /translation="MTFDRHVAPILFDRCAVCHRPEGAAPFALLTYDDARRRARQIVE
                     VTASGYMPPWLPAPGRGEFVGERRLAEAERSTLRRWLEQGTPPGDAPEAVVAPRFDSG
                     WTLGPPDLELAMTEAFTIPASGPDVYRNFVLPVPIDRTRYVRAVEFRPGSPTTVHHAR
                     VLLDPTPVSRQRDAESPAYGFAGMDSGAAAAPDGHLLGWTPGKVPAAAPADLAWRLDP
                     GDDLVLQLHMVPSGKPESIQASIGLYFTDRRPTRRAYALILGSREIDIPAGAAAHVVR
                     DRYELPIDVEALAVYPHAHYIAREMRVWAELPTGERRWLIHIPEWDFNWQDEYRFARG
                     VSIPRGSALVMEFVYDNSADNVRNPSSPPRRVRYGANSTDEMAEVMIQVLPAGEAERA
                     RLEDDFERFRVQKAIAYRQERLAADPLDAASHAALASSYLYLGQAALAVPHLEAALRI
                     GPESAGLHQNLGYALRRAGRVDEAARHYRRALELAPDSAEAAFDLAQIFRERGQAEQA
                     LALYRRVLRLRPESARTCVAIAELILDDSRPAAEEIAEAVRMAELAVRLSRGRDRATL
                     ELLATAYDAAGLPDRAREVRATLPPTAN"
     gene            complement(2682..4022)
                     /locus_tag="VD788_09905"
     CDS             complement(2682..4022)
                     /locus_tag="VD788_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HXV76621.1"
                     /translation="MLRCGATLGRLVRTTLIAPALLAAPEQTGAGPAAVPRNLVLVSI
                     DTLRADRLGCYGHPRPTSPVIDALARDGVIFDDASAPSPWTKPSHGALLSGRYPSRNG
                     LTTMAGELDAQTPHLAELLRDAGFQTVAIVNSKLLTQGGLARGFDHVEYLPYRQGDRR
                     GSPVSSTAVELLDRRDQRRRLFAFIHYMDVHSDYVSLPRYEEMFVRPYDGYADGSTQQ
                     LYRFSLGLFDLDRRDADHLLDLYDAGIRQIDDRIGRLVDYLKQQGLLDETLVVVLSDH
                     GEEFLEHGSVLHGHSQFQEVVRVPLLLCGPTVPRGVRVSTPVSLVDVMPTVIDLLGLP
                     LPDRLDGLPLRGAWDGSSALPPGRELFFEADVVWPPPGPGLSPLGPYRAVRSGRFKLH
                     YDVDTKHAGLFDLERDPGETVNVIADHPNEARRLWRRLERFLAGPKPADETGSR"
     gene            complement(4033..>4896)
                     /locus_tag="VD788_09910"
     CDS             complement(4033..>4896)
                     /locus_tag="VD788_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXV76622.1"
                     /translation="ATAPDTARSLTGLLTGYPFAAEPGAAPRPSLGELFSRLGYRTYA
                     LVEDGAVDRQLAPGFEEWNDGLDGADPIPQLADALARRAPSVGLVYLAGPDSLAPLSS
                     GLAELPGYDRTLVVLTSDHGEPDPAAPVERGFRDSVVRVPLLVKFPAGGKPETVPSRT
                     GSITSSIDLLPSLLALAGKDISGALPGSNVLDGYFSGSAFITAGDAWAFVQDNHKVVV
                     RGEKAWVFDLAADPAETEDLTASRPDLARGLLKMAVEMRGSLSTDAVSAVDEGMDEET
                     LEHLRSLGYIQ"
BASE COUNT          771 a         1713 c         1720 g          692 t
ORIGIN      
        1 ggaggaagac ggcggcgggg tccgcttcgg gcaggtgctc gtcggccagg cgaacgatcg
       61 cctggagcgt ctcgcgcaac gagctcttgg ccagcaggtt ctgcgcgatc gagacgaagc
      121 cccccggttc cgtgtggttg aagtactcga tcagcgtgca ccgcggatcg agcgggacga
      181 ggaaccaggc gccgtaggtc tcctcgagtg gcggcaactc gtccgcgatc atgctgatcg
      241 gaggctgcgc ggcgtcgacc tggtccgaca cgccgcgcca gctgagctcc cagatcctgc
      301 ccgccgagtc gcgatagagc tcgccgttca tctcgacgcg gctgacccac cagcggccga
      361 taccggcctt cttgaagtac tggaacaacg tccgctcgat cccccgcggc gttccaccga
      421 tcacctcgga atgccgcagc ggcaggtact tgccgtcgag cgcgtggtgc ggctcgtcgt
      481 tcaacgcttt ccagatcgtc tcgaccggcc ggtcgatgac gagcaccgcg cgcatcgtct
      541 tcccgtcgcg cgacgcgacc ggtctgccga cgttcgccgc cgccttgtcg tagagggcga
      601 acgggggatc ggcgccgaag cgagcggtgc gcgaaccgcg acggacgtca acgagacgca
      661 acgtcgtcga tacctcgttg cgagtcgggc aggtcgcctc ggggtcggcg gcccacgacg
      721 gcatgccggt gacgatcgcc agccaaacca gcatcgaacg gatcgacaaa ccggaggcct
      781 ccgtccccaa ggttaacacg cgcgcccgcc tcgtcagttc gcggtcggcg gtaacgtcgc
      841 gcggacctcg cgcgcgcggt cgggcaggcc ggccgcgtcg taggccgtcg ccaacagctc
      901 gagcgtcgcg cgatcgcgac cccggctcaa tcgcacggcg agctcggcca tccgtaccgc
      961 ttccgcgatt tcctcggcgg cgggtcgcga gtcgtccaag atcagctcgg cgatcgccac
     1021 gcacgtccgg gccgactccg ggcgcaatcg cagcacgcgg cgatacagcg cgagcgcctg
     1081 ctcggcctgc ccccgctcgc gaaagatctg cgccaggtcg aatgccgctt cggccgagtc
     1141 cggtgccagc tcgagcgcgc gccggtagtg tcgcgcggcc tcgtcgactc gcccggctcg
     1201 acgcagcgcg tagcccagat tctggtgcag accggcgctt tccggaccga tgcgcagcgc
     1261 cgcctcgaga tgcgggacgg cgagcgccgc ctggccgaga tacagatagc tcgaggccag
     1321 cgcggcgtgg ctcgcggcgt ccaaggggtc ggccgccagt cgttcctgcc ggtaggcgat
     1381 cgccttctgg acacggaagc gctcgaagtc gtcctcgagc cgggcgcgct ccgcctcgcc
     1441 cgccggcagc acctggatca tcacctcggc catctcgtcg gtcgagttgg cgccgtagcg
     1501 cacccggcga ggcgggctgc tcggattgcg cacgttgtcc gccgagttgt cgtagacgaa
     1561 ctccatcacc agcgccgacc ctcgtgggat cgacacaccg cgggcgaagc gatactcgtc
     1621 ctgccaattg aaatcccatt ccgggatgtg gatcagccat cgccgctcgc cggtcgggag
     1681 ctcggcccag accctcatct cacgggcgat gtagtgcgcg tgagggtaaa ccgccagcgc
     1741 ttcgacgtcg atcggcagct cgtagcgatc gcgaaccacg tgggccgccg cgcccgccgg
     1801 gatgtcgatc tcgcgcgacc cgaggatcag cgcgtacgcc cgccgggtcg ggcgacggtc
     1861 ggtgaaatag agaccgatcg aggcctggat cgactccggc ttcccgctcg ggaccatatg
     1921 caactgcaag acgaggtcgt cccccgggtc gaggcgccac gccaggtccg cgggggcggc
     1981 ggccggcact ttgcccggcg tccatccgag caggtggccg tcgggcgcgg ccgccgcacc
     2041 gctgtccatt cccgcaaagc cgtatgccgg cgattcggcg tcgcgctgac gtgacaccgg
     2101 tgtcggatcg agcagcactc gcgcgtgatg cacggtcgtc gggcttccgg gtcggaactc
     2161 gacggcccgc acgtatcgcg tccggtcgat cgggacggga aggacgaagt tgcggtagac
     2221 gtcgggtccc gacgccggga tcgtgaacgc ctccgtcatc gccagctcca gatccggggg
     2281 accgagtgtc caaccggaat cgaaccgggg agcgacgacc gcctccggcg cgtcgcccgg
     2341 cggggtgcct tgctcgagcc agcggcggag cgtcgagcgt tccgcttcgg cgagccgccg
     2401 ctcgccgacg aactcgcccc gaccgggcgc ggggagccac ggcggcatgt atccgctcgc
     2461 cgtgacctcg acgatctgcc gagcccgccg cctggcgtcg tcgtaggtca gcagcgcgaa
     2521 cggcgccgca ccttcgggac gatggcagac cgcgcagcgg tcgaacagga tcggcgcgac
     2581 gtggcggtcg aaggtcaccg gttcgccggg cgatccgcac ccgacgcaag acgccaccgc
     2641 cgccgcgacc gaccagacgg cccggcgact cgccgcgccg ctcaacggga accggtctcg
     2701 tcggccggtt tcgggccggc gagaaagcgc tcgagacggc gccacaaccg gcgcgcctcg
     2761 ttcgggtggt ccgcgatcac gttgaccgtc tcgccggggt cccgctcgag atcgaacagg
     2821 ccggcgtgct tcgtatcgac gtcgtagtgc agcttgaatc ggccgctgcg caccgcgcga
     2881 tagggtccca gcggcgagag ccccggcccc ggcgggggcc aaacgacgtc ggcctcgaag
     2941 aacaactcgc gaccgggcgg gagcgccgag ctgccgtccc acgcgccgcg cagcggcaac
     3001 ccgtcgagac ggtccggcag gggcagaccg agcaggtcga tcaccgtcgg cattacgtca
     3061 acgagcgaca cgggcgtcga cacgcggaca ccgcgcggga ccgtcggccc gcacagcaac
     3121 agcggcacgc gcaccacctc ctggaactgg ctgtgaccgt gcaacacgct gccgtgttcg
     3181 agaaactctt cgccatggtc cgacagcacg acgaccaggg tctcgtcgag cagcccctgc
     3241 tgcttcaagt agtcgacgag gcggccgatg cgatcgtcga tctgccggat accggcgtcg
     3301 tacaggtcga gcaggtggtc cgcgtcccgc cggtcgaggt cgaacagccc cagcgagaat
     3361 cggtacagct gctgtgtcga gccgtcggcg tagccatcgt agggacgaac gaacatctcc
     3421 tcgtagcgcg gcagcgagac gtagtcgctg tggacgtcca tgtaatggat gaaggcgaac
     3481 agacggcgcc gttggtcgcg gcggtcgagc agctcgaccg cggtgctgct gaccggcgat
     3541 cctcgccggt cgccttgccg atagggcagg tactcgacat ggtcgaagcc gcgagcgagc
     3601 cctccctgcg tcagcagctt gctgttgacg atggcgacgg tctggaaccc cgcgtcccgc
     3661 aacagctcgg ccaggtgcgg cgtttgtgca tccaactctc cggccatcgt cgtcaatccg
     3721 ttgcgactgg ggtagcggcc gctcagcagt gcgccgtgcg acggcttggt ccacggggac
     3781 ggcgccgaag cgtcgtcgaa gatcactccg tcgcgggcga gggcgtcgat caccggcgac
     3841 gtgggacggg gatgaccgta gcatccgagc cgatcggcac gcagcgtgtc gatcgagacg
     3901 aggaccaggt tgcgcggaac cgccgccggc ccggcgccgg tttgctccgg ggcggcgagc
     3961 aaggccgggg cgatgagcgt cgttcgaacg agccggccga gcgtcgcacc gcaccggagc
     4021 atcgtccctc acttactgga tgtagcccag gctgcgaagg tgttcgagcg tctcctcgtc
     4081 catcccctcg tcgaccgccg agaccgcgtc ggtcgagagg gacccgcgca tctcgaccgc
     4141 catcttcaac agaccgcgcg cgaggtcggg ccgactcgcg gtgagatcct ccgtctccgc
     4201 ggggtccgcg gcgaggtcga agacccatgc tttctctccg cggacgacca ccttgtggtt
     4261 gtcctggacg aaggcccacg cgtcgcccgc ggtgatgaag gcactgcccg agaagtaccc
     4321 gtcgagcacg ttcgagcccg gcaatgcacc ggagatgtcc ttaccggcca gggccagcag
     4381 cgacggcagc aggtcgatgc tcgacgtgat cgagccggtc cgcgacggca cggtctctgg
     4441 cttcccgccg gccgggaact tgaccagcag cggaacccgg acgaccgagt cgcggaagcc
     4501 gcgctcgacc ggggcggcag gatccggctc accgtggtcg gaggtcagca cgaccagggt
     4561 ccggtcgtag cccggcagct cggcgagccc gctcgaaagc ggcgcgagcg aatccggacc
     4621 ggcgaggtag acgagaccga cggacggcgc gcgccgcgcc agtgcgtcgg ccagttgggg
     4681 aatcgggtcg gcgccgtcca aaccgtcgtt ccactcctcg aagccggggg cgagctgccg
     4741 gtcgacggcg ccgtcctcga ccaaggcgta ggtccggtag ccgagccgcg agaacagctc
     4801 gccgaggctc ggccgtggcg ccgctccggg ctcggccgcg aaggggtagc cggtgagcag
     4861 accggtcagc gagcgcgcgg tatcgggcgc cgtcgc
//
LOCUS       DASPGN010000345         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_296950, whole genome shotgun sequence.
ACCESSION   DASPGN010000345 DASPGN010000000
VERSION     DASPGN010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296950"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1672
                     /locus_tag="VD788_09915"
     CDS             <1..1672
                     /locus_tag="VD788_09915"
                     /inference="COORDINATES: protein motif:HMM:NF009379.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXV76623.1"
                     /translation="LDAHQIPHLVFINKMDRSEVRYRDLIQSLRDLSDRPVIPHQYAI
                     GRADELVGYIDLVTEQAYSYNPGAPSDRIELPTEYREREHAARTEMLETLADFDDDLM
                     EMLLDEQEPPADDIVRHLQKSLGADQVVPVFMGVAERDMGVRRLLEALLKETPEPSVT
                     AARVGVDPNGPTVVQVLKTYHLPHSGKLSLVRVWAGEVKDVMTLGGMRVGGVYRMFGA
                     HHENVGAAGPGEIVGLGRLEEAKTGQTLTNGAAAPALPTAESTPPMYAFAIAADNRGD
                     EVKLSGAIAKLIDEDPSLRFEQDPELQESLLWGQGEMHLRVAMDRLRSKYNIAVHGRP
                     PQTPYRETIRKSTQSHGRHKKQSGGHGQFGDVKIEIRPRARGEGFEFVDKIVGGAVPR
                     QYIPAVEAGAREYLRRGPLGFPVVDVSVTLYDGQFHSVDSSELAFKMATALALKEGLA
                     KCDPVLLEPILAVEISVPSDVTSKVLQLVTQKRGQILGYDAKDGWTGWDRISVHIPQA
                     EMHDLIVNLRSLSQGVAFFSWTYDHLNPVPDKQAETVVAHRQAELHPS"
     gene            complement(1745..>2101)
                     /locus_tag="VD788_09920"
     CDS             complement(1745..>2101)
                     /locus_tag="VD788_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HXV76624.1"
                     /translation="AEADDVPPGVHGFHIHETGQCEPPDFESAGGHYNPTDKQHGWDN
                     PEGRHAGDFANVHVQDDGKLAVEYFTDAVTLGEGESTLFDDDGSAVVVHEGPDDYQSD
                     PAGHAGNRIACGVISR"
BASE COUNT          332 a          745 c          723 g          301 t
ORIGIN      
        1 cctcgacgcc caccagatcc cgcacctcgt cttcatcaac aagatggacc gctcggaggt
       61 gcgctaccgc gatctgatcc agagcctgcg cgacctttcg gaccggccgg tgatcccgca
      121 ccagtacgcg atcgggcgcg ctgacgagct ggtcggctac atcgacctgg tgacggagca
      181 ggcctacagc tacaaccccg gcgcgccttc ggaccggatc gaactgccga ccgagtatcg
      241 cgagcgcgag cacgcggcgc gcaccgagat gctggagacg ctagccgact tcgacgacga
      301 cctgatggag atgctgctgg acgagcagga gccgccggcc gacgacatcg tccgccacct
      361 gcagaagtcg ctcggcgccg atcaggtcgt gccggtgttc atgggcgtcg ccgagcgcga
      421 catgggcgtg cgccgcctgc tcgaggcgct gctcaaggag accccggagc cgagcgtcac
      481 cgcggcccgg gtcggcgtgg atccgaacgg cccgaccgtg gtccaggtgc tcaagaccta
      541 ccatctgccg cattcgggca agctgtcgct cgtgcgcgtg tgggcgggcg aggtcaagga
      601 cgtgatgacg ctgggcggca tgcgggtcgg cggcgtctac cgcatgttcg gcgcgcacca
      661 cgagaacgtc ggcgctgccg gccccggcga gatcgtcggc ctcggccggc tcgaggaagc
      721 gaagaccggg cagacgctga ccaatggcgc cgccgcgccc gcgctgccga ccgcggaatc
      781 gacgccgccc atgtacgcct tcgcgatcgc cgccgacaac cgcggcgacg aggtcaagct
      841 gtccggcgcg atcgccaagc tgatcgacga ggaccccagc ctgcgcttcg agcaggaccc
      901 cgagctgcag gagagcctgc tctggggcca gggcgagatg cacctgcggg tcgccatgga
      961 ccggctgcgc tcgaagtaca acatcgccgt gcacggccga ccgccgcaga cgccttatcg
     1021 ggagaccatc cgcaagtcga cccagagcca cggccggcac aagaagcaga gcggcggcca
     1081 cggccagttc ggcgacgtca agatcgagat ccggccgcgc gcgcgcggcg agggcttcga
     1141 gttcgtcgac aagatcgtcg gcggcgcggt gccgcgccag tacatcccgg cggtcgaggc
     1201 gggtgctcgg gaatacctgc gccgtggccc gctcggcttc ccggtggtcg acgtcagcgt
     1261 caccctgtac gacggccagt tccacagcgt cgacagctcg gagctcgcgt tcaagatggc
     1321 gaccgcgctc gcgctgaagg agggcctggc caagtgcgac ccggtgctgc tcgagcccat
     1381 cctcgcggtc gagatctcgg tgccctcgga cgtcacctcg aaggtgctgc agctggtgac
     1441 gcaaaagcgc ggccagatcc tgggctacga cgccaaggac ggctggacgg gctgggatcg
     1501 gatctccgtg cacatcccgc aggcggagat gcacgacctg atcgtcaacc tgcgctcatt
     1561 gagccagggc gtcgccttct tcagctggac ctacgatcac ctcaacccgg tgccggacaa
     1621 gcaggccgag accgtggtgg cgcatcggca ggccgagctg cacccgtcct gagagggcgt
     1681 ccggcccgcg gcccgaccac cggccgcacc gcccgcctgc ccgaggctcg gcgcggcgac
     1741 gacgttaccg ggagatgacc ccgcaggcga tccggttgcc ggcgtggccg gccgggtcgc
     1801 tctggtagtc gtccgggccc tcgtgtacca cgaccgccga cccatcgtcg tcgaacagcg
     1861 tgctttcgcc ctcgcccagc gtcaccgcgt ccgtgaagta ctcgaccgcc agtttgccgt
     1921 cgtcttgcac gtgcacgttg gcgaagtcgc ccgcgtgacg gccctcgggg ttgtcccagc
     1981 cgtgctgctt gtcggtcggg ttgtagtggc ccccggccga ctcgaaatcc ggcggctcgc
     2041 actgcccggt ctcgtggatg tgaaacccgt gcacgccggg cggcacgtcg tccgcctcgg
     2101 c
//
LOCUS       DASPGN010000346        11607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1465537, whole genome shotgun sequence.
ACCESSION   DASPGN010000346 DASPGN010000000
VERSION     DASPGN010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11607
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465537"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VD788_09925"
     CDS             <1..315
                     /locus_tag="VD788_09925"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXV76625.1"
                     /translation="GPVPATRKLLDRVGIGLDRIELVELNEAFAAQALACLRELGLAD
                     DAEQVNPNGGAIALGHPLGMSGARLLATATYELERRRARYALCTMCIGVGQGIAMLLE
                     RV"
     gene            346..1527
                     /locus_tag="VD788_09930"
     CDS             346..1527
                     /locus_tag="VD788_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXV76626.1"
                     /translation="MEPASDWPLDPTIHFLNHGSFGSCPTPVLRYQSELRRRMERQPV
                     QFFVRDLEALLDGAREALARFVHVAADDLVPLSNATAGVNAVLRSLDLGATDELLVTD
                     HEYNACRNALDYVASRAGARVVVAAIPFPLTSPDEVVEAVLGRVTRRTRLALLDHVTS
                     QTGLVLPIERLVAELASRGVETLVDGAHAPGMLDLDLSRVGAAYYAGNCHKWLCAPKG
                     AGFLYARRDLQPAVRPIAISHGANTPRPGRSRFQTEFGWLGTDDPTPFLCVPRSLRFL
                     ADALPGGWPALRRRNRGLVLEGRRVLCEALDRGLPCPDEMIGSLASIPLPAAPPAEGR
                     SALYGDPLQEVLLGRFSIEVPIVAWPAAPERLVRISAQLYNRPEQYRLLAAALGEVLR
                     A"
     gene            2092..2529
                     /locus_tag="VD788_09935"
                     /pseudo
     CDS             2092..2529
                     /locus_tag="VD788_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
     gene            2798..4987
                     /locus_tag="VD788_09940"
     CDS             2798..4987
                     /locus_tag="VD788_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76627.1"
                     /translation="MRNKLITTIVLVAAACLPPVAADVPPLLSYQGRIVDSMGSPVVD
                     PTLVRFRVYRGGDGTSVFSSGLVVYDELVWIEPSPNGVFSHSVGSGTAAPSCDDGPCA
                     LSAGDFGDGVEPIWIEVRVDPDGEAGTPDDDLLLPRTRIGTVGFAYRVASLDGAEGGA
                     LAGAVTADTLVGRTGATFGDGSASDVALTFDGAAGDPTIRWVDSLSEFAFSASTRHEA
                     GLSLAAGRSIAIGPFDNPTEGAYLRMNHTAGLAADGNLDGTPDANHVFALCYNCAEGS
                     SGAELPSEYTMAYQWAMTNAPLDGQRSTSQTWTFRGPGMPAFRPYLFSMDLDAAGGYA
                     SPTAKWTFSNSPGSVALHINQNGNVKIGADSPQPTYPLDVEGTIHATEDLRLEPSRGV
                     TFGSTPALVDDSASSRLLVGGGYTRVQLGNGTGEVNLNFGSDLFRPRIQWLPAFQRFT
                     VTDETLFSEGITIGPLKTISYGPFTNPVPDTYIRYYKTADQFADGNLDGLPDEDHVVG
                     VCYNCGEGGSGGPQVTSEYALRAQWEMSYAPVDGERVVEHNWNFLALGKPLFRPLLFY
                     LDVDQDGNFAEPWAKWTFSTERGKVAFHINQNGNVVVGSDSPQPTRQLEVRGDAAVEG
                     GLVLGACDDAAAAGTLNIASCNLLTVHGASTVNRVNTCDDDEVGRVLYLLCGNDATVL
                     CDGDGSRCSSGNLRLAGDDADFVCSPDDVLTLMCDGFNWRQVAVSAN"
     gene            5053..7257
                     /locus_tag="VD788_09945"
     CDS             5053..7257
                     /locus_tag="VD788_09945"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HXV76628.1"
                     /translation="MNDGIRTLLASTAIAVAVSCPAARAAEGMWTPAQMTEVGGRLAA
                     AGLELPAATLSDLTAHPMNAVISLGGCTASFVSADGLVVTNHHCATRAIQHNSTEERN
                     LLETGFLAGSRDEELFAGPGSRLLVTVAADDVTDRILAELDAGLDGYARQQEIEAREK
                     RLVADCEKDPGHRCRVASFHGGLQYVRIKQLEIRDVRLVYAPADGVGNYGGEIDNWMW
                     PRHTGDFSFYRAYVDRDGKAADHAPTNVPYRPKHHLTIAGGGLGTGDFVIVAGYPGRT
                     NRYRLADEVHGAFEWYYPRRRAALEQWLAVIERETAARPDARIRYAGRVSGLNNSIKN
                     YQGMLDSYAKVDLVGRKRKLESELAGWIGEDAERAARCGDALGELQRLVDLEQADRQR
                     ELYYASLAREADLYDAARTLYRLSRERRKADSDREAGYQQRDERRIRERLTRLERTFD
                     AEVDRAVWRAMLLDYAALPPDQRVGAFDAWFGFDGEAADPDLLDRRLDEMYGATRLGR
                     TETRLQWMDADAPAFDESEDPFIRLAAATYPSDIELERKEKELAGRFTVARPRYMECL
                     IAYRESLGQPVYPDANGTLRLTFGQVRGYSPRDAVVFEPFTTVGGIVEKETGNDPFDA
                     PPPLLRAIAERRFGPYLDPRLGSVPVDFLSDVDTTGGNSGSPTLNARGELVGLLFDGT
                     YESIISDWDFLPEVTRSIHVDIRYALWVMEFVDGARSLVREMSADGGPSTGN"
     gene            7431..>11607
                     /locus_tag="VD788_09950"
     CDS             7431..>11607
                     /locus_tag="VD788_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV76629.1"
                     /translation="MRRSRRPGVGSYLAIGAIAVGGLWFAVSGGSGGDPDRGRAARAP
                     AFVPGELLVRFKDAAAEGERTRVRSLVGGRRLRRLGRALEQLKLDDGRSTQQALERLR
                     RDPAVEYAEPNYLYHVDNLPDDPLFPELFGLRNTGQTGGTPGADIHAEEAWDLTTGSR
                     EVVIGVIDSGVDYNHPDLAPNIWINPDEIPQNGIDDDDNGYVDDVRGWDFVNDDNDPF
                     DDQGHGTHVAGTIGAAGDNGLGVTGVAWKVRLVPLKFISAFGSGTTADAVAAVEYATA
                     IGADATNNSWGGGAYSLALDEAIQAAAEAGVVFVASAGNQGLNTDLLPHYPSSYSAPN
                     VISVAAVNHDDVRPVFSNVGPRSVDLGAPGVSVLSTTPGGTYGLNTGTSMAAPHVTGA
                     VALIRSLAPEIGVVEIRQLLMDHVDPTPTLGPLIASGGRLNVARSFAARDQLEPGAVR
                     QLTSVGSTSNAIELSWIATGDDGDDGTATTYELRFAPWPIDESNFALASRAWQTPRPA
                     PSGSAESFELTGLASGSDYHVALRAVDEWGNRGPLALALPAATLPPPVFASSPVRFDL
                     TLFSGERASRTIDLTNAGPGTLDWQVDSADLSWIGLSPQSGRLLSGETTLVALEIDTR
                     DLAPGVHQVTVEIGSNDPLRPLVPHPVTVEVIGAPAIEVEPSAVDAGSVIVGASTNVV
                     LTVRNFGSEPLTVDATPGGTTELTVTPSQLELAPGEQRTLDLRWSPTVAGPFDAVLTL
                     ATNVPGVPLLVVPVHGDAAPAPAIAIEPSALGETLLAGTSSVRTLRIHNPGDGPLVVD
                     FRFEPGDDPGPSLVSPVVNGGFESGDFTGWEATTNNLFTRGPWIVAGPGVGSFENSQP
                     IEGSFDALNAFEGQAGLRFDLFQSLAIPVGATRAELGYFDRIQWNDFGAPGEIERFYR
                     ATIEDDHGQRLDTIVEREILFDGTGYTDLGWQRRSIDLAHHAGRTVRLHIREGIQQSL
                     TGLAQIEFDDFRMELEGFPAWFRPQPKRVTVAPGETVDVELVFDAAGLLSAAFDGRIR
                     LETNVPSTPRLFVDIDLSVYGVPRMTLRGRPFVVESTRTFSTAGATTEHLLSLAAPAS
                     GGGLLQLVVEADLGQAGESALLRVEGVELGSIGNTGGDCAPATAEFAVDAERLAAIAA
                     DGVLEATVTNTPSVAVICAANRHRLRLTYDTPAETVDFGTRFVGETVELPILIENSGS
                     APLELTSITSDRPAYTLSAASAQLPVGATMSLAVRFTAGVAGHFDGTLTIVGNDPERP
                     RFDVALTATAIDPPTIELEPAALDERLPVGGATERVLRLTNHGDAPLDYSIDLSSAGE
                     SFVELVPSGGSVAADATADLLVRLDAVGLAPASYDATLTVRAQLESIPAVVVPLRLTV
                     DSAPDIEVHGPSIR"
BASE COUNT         1735 a         4076 c         4060 g         1736 t
ORIGIN      
        1 ggcccggtgc ccgcgacgcg caagctgctc gatcgagtcg gtatcgggct ggaccgcatc
       61 gagctcgtcg aactcaacga ggcgttcgcg gcgcaggcgc tcgcctgcct gcgcgagctc
      121 ggtctcgccg acgacgcgga gcaggtcaac cccaacggcg gtgcgatcgc gctcggtcat
      181 ccgctcggca tgagcggcgc ccggctgctc gcgacggcga cctacgagct cgaacggcgg
      241 cgcgcgcgct acgcgctgtg cacgatgtgc atcggcgtcg ggcagggcat cgcgatgctg
      301 ctcgaacggg tctgagcgcc ggtcgggcag cggtacaatc ggcggatgga accggcgtcc
      361 gactggccgc tcgatccgac gatccacttc ctcaatcacg gctcgttcgg ctcgtgcccg
      421 acgccggtgc ttcgatacca atccgagctg cgccggagga tggagcgcca gccggtccag
      481 ttctttgtcc gcgacctcga ggcgctgctc gatggtgcgc gggaagcgct cgcgcggttc
      541 gtccacgtcg cagccgacga tctcgtgccg ctgtcgaacg cgacggccgg ggtcaacgcc
      601 gtgcttcgtt cgctcgacct cggcgcgacc gacgagctgt tggtcaccga ccacgagtac
      661 aacgcctgcc gcaacgcgct cgactacgtc gcctcgcgtg cgggcgcccg cgtcgtcgtc
      721 gcggcgatcc cgttcccgct gacctcgccg gacgaggtcg tcgaggcggt tctcggacgc
      781 gtcacccgcc gcacgcgttt ggcgctgctc gatcacgtga cgagtcagac cggtctcgtg
      841 ctgccgatcg agcggctggt cgccgagctg gcgagccgcg gcgtcgagac gctggtcgac
      901 ggagcccacg cacccggcat gctcgatctc gacctgtcgc gggtcggggc ggcgtactac
      961 gccggcaact gccacaagtg gctgtgcgcg ccgaagggcg caggctttct ctacgcccgc
     1021 cgcgacctgc agccggcggt ccgcccgatc gcgatcagcc acggagcgaa cacgccgcgc
     1081 ccgggtcgct cccgtttcca gaccgagttc ggttggctcg gcaccgacga cccgacgccg
     1141 ttcctgtgcg ttccgcgctc gctgcggttc ctggcggacg cgctgccggg cggctggccc
     1201 gcgctgcgac ggcgcaaccg cgggctggtg ctcgaaggac ggcgggtgct gtgcgaggcg
     1261 ctcgaccgcg gtctgccgtg tccggacgag atgatcgggt cgctggcttc gataccgctg
     1321 ccggccgcgc cgccggccga ggggcgctcg gcgctctacg gcgacccgtt gcaggaggtg
     1381 ttgctcgggc gcttctccat cgaagtgccg atcgtcgcct ggcccgcggc gcctgagcgc
     1441 ctcgtgcgca tctcggcgca gctgtacaac cgcccggagc agtaccggct gctcgccgcc
     1501 gcgctcggcg aggtattgcg ggcgtagccc gaacccgccg gcccgagagg tattgtaaga
     1561 gccctcccgg cagatccccc acggcccggc tagccgcacg tccggccgag cggtatatca
     1621 atgacggcgg cgctcgatgc gccgcatttg ttctggggcg gctcggcccc ccgggcgcgg
     1681 gaaagtgtgt cgatgcttcg ggatctgctg cgacgtgcgt gcggtgccgc gctcgtgacc
     1741 ctcggggtcg cggcgctcgc gccgtcgccg gtcgcacagc ccgctccacc gggcgagctc
     1801 ggaccctcgc tccgcttcct cgacccggtg acgttgacct gggacgagga aggaaacgcc
     1861 gacagctaca acgtctacaa gggtctggtc gcgagcgacg ccgactggca gtacaaccac
     1921 gtctgtctgg cgctcgaggt cggcgggacg cagatcgtcg acagcgcgat cccgtccgtc
     1981 ggcctgacgg cctactacct cgtcagcaag gagaaccggt tcggtgaggg gccgctcggc
     2041 tcgcaggccg gcaaggtgct gcgcccgaac cccgagccgt gcgtcgattc ggacggcgac
     2101 ggagtggccg acaacctcga caactgtccg aacgatccga acgccaacca ggccaacgcc
     2161 gacttcgacc tcttcggcga cgtctgcgac gacgacgacg acaacgacgg gctggacgac
     2221 gacaccgagg attcgctggg caccgatccg accgatcccg actcggacga cgacggactg
     2281 accgacgccg aggaggtgct ggtcaccgga accgatccgt tgtcgcccga caccgatgac
     2341 gacggggcac cggacgcgca cgacaactgt ccgaccgcgt ccaacgcgaa ccaactggac
     2401 tcggacgaag acggcccggg cgacgcttgc gacaattgcc cgttcacctt caacccggac
     2461 cagagcgagt ccgacgggga cggcccgggc gacgcctgcg acaattgccc gttcaccttc
     2521 aacccggacc agagcgactt gaacggcgac gggatcggcg acgcgtgcgg cggcttcgtc
     2581 gacgtttggg tgctcgatgc cggcggcaca ccgagcgctg gcgcgggcta tgcgatcgac
     2641 cgatgttcga tcggtcaggt cgccgcgggc gcgtcgaccg ccgtgctggt ccgcatcgaa
     2701 gcgggcttcg tccacgacgg cacgatccag ggcgcgccat gagacggaac gacgggccgg
     2761 gaccgatcgg cggttcccgg cggggagacg gcgaaagatg cgaaacaagc tgatcacgac
     2821 gatcgtcctc gttgccgcgg cgtgtctgcc gccggtcgcc gcggacgtcc cgccgctgct
     2881 ctcctaccag ggtcgcatcg tcgattcgat gggctcgccc gtcgtcgacc cgaccctcgt
     2941 ccgcttccgc gtctaccgcg gcggcgacgg cacgtcggtc ttctcgagcg gcctcgtggt
     3001 ctacgacgag ctggtctgga tcgagccgtc gccgaacggc gtcttctcgc actcggtcgg
     3061 ctcggggacg gccgcgccgt cgtgcgacga cggaccgtgc gcgctgagcg cgggtgactt
     3121 cggcgacggc gtcgagccga tatggatcga ggtacgcgtc gaccccgacg gcgaggcggg
     3181 tacgcccgac gacgacctgc tgctgccgcg gacgcggatc gggacggtcg gcttcgccta
     3241 ccgcgtggcg agtctcgacg gtgccgaggg cggcgcgctg gccggtgcgg tcacggccga
     3301 cacgctggtc ggacggacgg gcgcgacctt cggcgacggc agtgcgagcg acgtcgcgct
     3361 gaccttcgac ggcgccgcgg gcgacccgac gatccgctgg gtcgattcgc tgtccgagtt
     3421 cgccttctcg gcctcgaccc ggcacgaggc ggggctgtcg ctcgccgccg gccgctcgat
     3481 cgcgatcgga ccgttcgaca acccgaccga gggcgcctat ctgcggatga accataccgc
     3541 cggcctggcc gccgacggca acctcgacgg cacgcccgac gcgaaccacg tcttcgccct
     3601 ctgctacaac tgcgccgagg gcagctcggg cgccgagctc ccgagcgagt acacgatggc
     3661 ctaccagtgg gcgatgacca acgcgccgct cgacggacaa cgctcgacgt cgcagacttg
     3721 gacctttcgc ggaccgggaa tgccggcctt ccgcccctat ctgttctcga tggacctcga
     3781 tgccgcgggc ggctacgcca gcccgaccgc caaatggacc ttcagcaact cgcccggcag
     3841 cgtggcgctg cacatcaacc aaaacggcaa cgtcaagatc ggcgcggatt cgccgcagcc
     3901 gacctacccg ctcgacgtcg agggaacgat ccacgccacc gaagacctgc ggctcgagcc
     3961 cagccgcggc gtgacgttcg gcagcacgcc ggcgttggtc gacgactcgg cctcgtcgcg
     4021 actgctggtc ggcggcggct acacgcgtgt gcagctcggc aacggcaccg gcgaggtcaa
     4081 tctcaacttc ggcagcgacc tgttccggcc gcggatccag tggctgccgg cgttccagcg
     4141 gttcaccgtg accgacgaga cgctgttcag cgaaggaatc acgatcggcc cgctgaagac
     4201 gatctcctac gggccgttca ccaaccccgt gccggatacc tacatccgct actacaagac
     4261 cgccgaccag ttcgccgacg gcaacctgga cggcctcccc gacgaggatc acgtcgtcgg
     4321 tgtctgctac aactgcggcg agggcggctc gggcggaccg caggtgacga gcgagtacgc
     4381 gctgcgcgcg cagtgggaga tgagctacgc gccggtcgac ggcgaacgag tggtcgagca
     4441 caactggaat ttcctggcgc tcggcaagcc gctgttccgg ccgctgctgt tctacctcga
     4501 cgtcgatcag gacggcaact tcgccgagcc gtgggccaag tggaccttca gcaccgagcg
     4561 cggcaaggtc gcgttccaca tcaaccagaa cggcaacgtc gtggtcggct ccgactcgcc
     4621 ccagccgaca cgtcagctcg aggttcgcgg ggacgcggcc gtcgagggcg ggctcgtgct
     4681 cggcgcgtgc gacgatgcgg cggcggccgg cacgctcaac atcgcgagct gcaacctgct
     4741 gacggtccac ggcgcgtcga cggtcaatcg cgtgaatacc tgcgacgacg acgaggtcgg
     4801 tcgcgtgctc tacctgctgt gcggcaacga cgccaccgtg ttgtgcgacg gcgacggctc
     4861 ccgttgttcg tccggaaatc tgaggctggc cggcgacgac gccgatttcg tctgcagccc
     4921 ggacgacgtg ctgacgctga tgtgcgacgg cttcaactgg cgccaggtgg cggtctcggc
     4981 caactgaggc gcgcgacgac gcttgcccgg cgcgccgggc cgatctagag tggggtcctc
     5041 gcgagtaacg acgtgaacga cggaatacgc actctgctcg catcgacggc gatcgccgtc
     5101 gccgtctcat gccccgcggc acgggccgcg gagggtatgt ggacgccggc gcagatgacc
     5161 gaggtcggcg ggcggctggc cgctgccggg ctcgagctgc cagctgcgac gttgagcgat
     5221 ctgaccgctc acccgatgaa cgcggtgatc agtctcggcg gctgcacggc atcgttcgtc
     5281 tccgccgacg gtctggtggt gaccaatcac cactgtgcga ctcgcgcgat ccagcacaac
     5341 tcgaccgagg agcggaacct gctcgagacc ggctttctcg ccggcagccg tgacgaggag
     5401 ttgttcgccg gccccggcag ccggctgctg gtcaccgtgg cggccgacga cgtgaccgac
     5461 cgcattctcg ccgagctcga cgccggcctc gacgggtacg cccgccagca ggagatcgaa
     5521 gcgcgcgaga agcggctggt cgccgactgc gagaaggacc ccggtcaccg ctgccgcgtc
     5581 gcgtcgtttc acggtggcct gcagtacgtg cggatcaagc agctcgagat ccgcgacgtc
     5641 aggctggtct atgccccggc cgacggtgtc ggcaactacg gtggcgagat cgataactgg
     5701 atgtggccac ggcacaccgg cgacttttcg ttctaccgcg cctacgttga tcgggacggc
     5761 aaggccgccg accacgcccc gaccaacgtg ccgtaccggc cgaagcacca cctgacgatc
     5821 gccggcggcg gactcggcac gggcgatttc gtcatcgtcg ccggctaccc cgggcgcacc
     5881 aaccgctacc ggctggccga cgaggtgcac ggcgcgttcg agtggtacta cccgcgacgc
     5941 cgcgcggcgc tcgaacagtg gctggcggtc atcgagcgcg agacggccgc ccggccggac
     6001 gcgcggatcc ggtacgccgg acgggtctcc ggtctgaaca actcgatcaa gaactaccag
     6061 gggatgctcg acagttacgc caaggtcgat ctggtcggta ggaaacggaa gctcgaaagc
     6121 gagcttgccg gttggatcgg agaagatgcc gagcgggcgg cccgctgcgg cgacgcgctc
     6181 ggcgagctgc aacgactggt cgacctcgag caggccgacc gacagcgcga gctgtactac
     6241 gcgtcgctcg cgcgcgaggc cgatctgtac gacgccgcgc gcacgttgta ccgattgagc
     6301 cgcgagcgcc ggaaggccga ttccgaccgc gaggccggct atcagcaacg cgacgagcgg
     6361 cgaatccgcg aacggctgac ccgcctcgaa cgcaccttcg acgcggaggt cgatcgggcc
     6421 gtctggcgcg cgatgctgct cgactacgcg gcgttgccgc ccgaccagcg ggtcggcgcg
     6481 ttcgacgcgt ggttcggctt cgacggcgag gcggccgatc cggacctgct cgaccgcagg
     6541 ctggacgaga tgtacggcgc gactcgcctg ggccggaccg agacgcggct gcagtggatg
     6601 gacgccgatg cgccagcgtt cgacgaaagc gaggacccgt tcatccgcct ggccgccgcg
     6661 acctacccca gcgacatcga gctcgagcgg aaggaaaaag agctggccgg acgcttcacc
     6721 gtcgcgcggc cgcgttacat ggaatgcctg atcgcctacc gcgagtcgct gggccagccg
     6781 gtctatcccg acgccaacgg cacgctgcga ctgaccttcg ggcaggtgcg gggctactca
     6841 ccgcgggacg cggtggtatt cgagccgttc accaccgtcg gcggcatcgt cgagaaggag
     6901 accggcaacg acccgttcga tgcgcctccg ccgctgctgc gggcgatcgc cgagcggcgc
     6961 ttcggcccgt acctcgatcc gcggttgggg tcggtcccgg tcgatttcct cagcgacgtc
     7021 gacacgaccg gcggcaactc cgggtcgccg acgctgaacg cccgcggcga gctggtcggc
     7081 ctgctgttcg atggaaccta cgagtcgatc atctccgact gggacttcct gccggaggtc
     7141 acgcggtcga tccacgtcga catccgctac gcgttgtggg tgatggagtt cgtcgacggg
     7201 gcacggtcgc tcgtgcgcga gatgagcgcc gacggcggcc cgtcgaccgg caactgagcg
     7261 cggcgtgccc gccctcccgg tgccgcgagt ttcaacgcgt cgcggtctgc gctgcatctg
     7321 ccccgcgccg ggtgccgatc cgcggttggc ccggtcccgt gttcgaccac tcgggggtat
     7381 agtctccata tcgtcgtcgc gcgcgcagga ccggggggag catggatccg ttgcgtcgct
     7441 cgaggagacc cggtgtcggg tcgtacctcg cgatcggcgc gatcgccgtc ggcgggctgt
     7501 ggttcgccgt ctcaggcggg tcgggcggcg acccggatcg gggtcgggcc gcccgagcgc
     7561 cggcgttcgt tcccggcgag ttgctcgtgc gcttcaagga cgcggcggcc gagggtgaac
     7621 ggactcgggt gcggtcgctg gtcggcgggc gacgcctgcg gcgcctgggg cgcgcgctcg
     7681 agcagttgaa gctcgacgac ggccggtcga cccagcaggc gctcgaacgg ctgcgtcgcg
     7741 atcccgccgt cgagtacgcc gagccgaact acctgtatca cgtcgacaac ctgcccgacg
     7801 atccgttgtt ccccgagctg ttcggcctgc ggaacaccgg tcaaaccgga ggaacgccgg
     7861 gcgccgatat ccacgccgag gaggcgtggg acctgaccac cggctctcgc gaggtcgtca
     7921 tcggggtgat cgacagcggg gtggactaca accaccccga cctggcgccg aacatctgga
     7981 tcaacccgga cgagatcccg caaaacggca tcgacgacga cgacaacggc tacgtcgacg
     8041 acgtgcgtgg atgggatttc gtcaacgacg acaacgaccc gttcgacgac cagggacacg
     8101 gtacccacgt cgccggaacg atcggcgccg cgggcgacaa cggtctcggc gtgaccggcg
     8161 tggcgtggaa ggtgcggctc gtaccgttga agttcatcag cgccttcggc tcgggcacga
     8221 cggccgacgc ggtcgccgcg gtcgagtacg cgacggcgat cggggccgac gccaccaaca
     8281 actcgtgggg tggcggcgcc tactcgctcg cgctggacga ggcgatccag gccgccgcgg
     8341 aggccggcgt cgtcttcgtc gcctcggccg ggaaccaagg gctgaacacc gacctgctcc
     8401 cgcactaccc gtcgtcgtac tcggcgccca acgtgatctc cgtcgccgcg gtgaaccacg
     8461 acgacgtgcg accggtgttc tcgaacgtcg ggccgcggag cgtcgacctc ggggcgcccg
     8521 gtgtatcggt gctgagcacg accccgggcg ggacgtacgg gctgaacacc gggacgtcga
     8581 tggccgcgcc gcacgtcacc ggcgcggtcg cgctgatccg ctcgctggcg cccgagatcg
     8641 gtgtggtcga gatccgccag ctgttgatgg accacgtcga tccgacgccg acgctcgggc
     8701 cgttgatcgc cagcggcggc aggctcaacg tcgcccgcag cttcgccgcg cgcgatcagc
     8761 tcgagccggg ggcggtgcgg cagctgacct ccgtcggctc gacgagcaat gcgatcgagt
     8821 tgagctggat cgcgacgggc gacgacggcg acgacggcac ggccacgacc tacgagctgc
     8881 gtttcgcccc gtggccgatc gacgagtcga acttcgcgct ggcgtcgcgt gcgtggcaga
     8941 cgccgcgacc ggcaccgtcc ggctcggccg agtcgttcga gctgaccggc ttggcgtccg
     9001 gcagcgatta tcacgtcgcg ctgcgcgccg tcgacgagtg gggcaatcgc gggccgctgg
     9061 ccttggcgct tccggcagcg acgctgccgc ctccggtgtt cgcgtcctcg cccgtgcggt
     9121 tcgacctgac gctgttcagc ggtgagcggg ccagccggac gatcgatctg accaacgccg
     9181 ggcccgggac gctcgactgg caggtcgact cggccgacct tagctggatc ggcctgtcgc
     9241 cgcagagcgg gcgattgctc tccggcgaga cgacgctcgt cgcgctcgag atcgacaccc
     9301 gcgatctggc gccgggggtc caccaggtca cggtcgagat cggcagcaac gacccgctgc
     9361 ggccgctcgt accccacccg gtgacggtcg aggtcatcgg cgctccggcg atcgaggtcg
     9421 aaccgtcggc ggtcgacgcc ggctcggtca tcgtcggcgc ctcgacgaat gtcgtgctca
     9481 ccgtgcgtaa tttcggcagc gagcccttga ccgtcgatgc gacgcccggc ggcacgaccg
     9541 agctgaccgt cacgccatcg caactcgagc ttgcgccggg cgagcagcga acgctcgatc
     9601 tccgctggtc gccgaccgtg gccggtccgt tcgacgcggt gctcacgctg gcaacgaacg
     9661 tgcccggcgt gccgttgctc gtcgtgccgg tgcacggtga cgcggcgccg gcgccggcga
     9721 tcgcgatcga accctccgcg ctcggcgaga cgctgctcgc cggcacgagc agcgtgcgca
     9781 ccctgcgcat ccacaatccg ggcgacgggc cgctcgtcgt cgacttccgc ttcgagccgg
     9841 gggacgaccc cggcccatcg ctggtctcgc cggtggtcaa cggcgggttc gagagcggcg
     9901 acttcaccgg ctgggaagcg acgaccaaca atctgttcac gcgcgggccg tggatcgtcg
     9961 ccggcccggg cgtcggatcg ttcgagaaca gccagccgat cgagggatcg ttcgacgcgc
    10021 tcaacgcgtt cgagggtcag gccgggttgc gtttcgacct gttccagtcg ctggcgattc
    10081 cggtcggcgc gacccgagcc gagctcggct acttcgaccg cattcagtgg aacgacttcg
    10141 gcgcgccggg cgagatcgag cggttctatc gcgcgacgat cgaggacgat cacggccagc
    10201 ggctcgacac gatcgtcgag cgcgagatcc tgttcgacgg gaccggctac accgacctcg
    10261 gctggcagcg gcgatcgatc gacctcgcgc atcacgccgg ccgcaccgtg cgactgcaca
    10321 tccgcgaggg aatccagcag tcgctgaccg gtctcgcgca gatcgagttc gacgatttcc
    10381 gcatggagct cgaaggcttc ccggcttggt tccgcccgca gcccaagcgg gtgaccgtcg
    10441 cgccgggcga gacggtcgac gtcgagctgg tgttcgacgc ggccggcctg ctgtccgccg
    10501 ctttcgacgg tcggatccgc ctcgagacca acgtaccgag cacgccccgg ctgttcgtcg
    10561 acatcgacct gtcggtctac ggcgtgccgc ggatgacgct tcgcgggcgg ccgttcgtcg
    10621 tcgagtcgac gcggacgttc tcgacggccg gcgcgacgac cgagcacctg ctgtcgctcg
    10681 cggcgcccgc gagcggcggc ggcctgctgc agctggtcgt cgaagccgac ctcggtcagg
    10741 ccggcgagtc ggcgctgctg cgcgtcgaag gcgtggagct ggggagcatc ggcaacaccg
    10801 gtggggactg cgcaccggcg accgccgagt tcgccgtcga cgccgagcgg ctggccgcga
    10861 tcgccgcgga cggcgtcctc gaagcgacgg tcaccaatac tccgtcggtc gccgtgatct
    10921 gcgccgccaa tcgtcatcgc ctccggttga cctacgacac gccggcggaa accgtcgact
    10981 tcggcacgcg cttcgtcggc gaaacggtcg agctcccgat cttgatcgag aactcgggct
    11041 ccgccccgct cgagctgacc tcgatcacga gcgatcggcc tgcctacacg ttgtcggccg
    11101 catcggcgca gctgccggta ggtgcgacga tgtcgctcgc cgttcgtttc acggcgggtg
    11161 tggccggcca tttcgacggc acgctgacga tcgtcggcaa cgatcccgag cgaccgcgct
    11221 tcgacgtcgc actgaccgca accgcgatcg acccaccgac gatcgagctc gagccggcag
    11281 cgctcgacga gcgcctgccg gtcggcggcg caaccgagcg cgtcctgcgg ctgaccaacc
    11341 acggggacgc gccgctcgac tactcgatcg acctgtcgtc ggccggcgag tcgttcgtcg
    11401 agcttgtacc gagcgggggc tcggtcgcgg cggatgccac cgccgatctg ctcgtgcggc
    11461 tcgacgccgt cgggctggct ccggcgagct acgacgcgac gctgaccgtc cgagcccagc
    11521 tcgaatcgat accggcggtg gtcgtcccgt tgcgcctgac cgtcgacagc gcacccgaca
    11581 tcgaggtcca cgggccgtcg atccggg
//
LOCUS       DASPGN010000347         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1465547, whole genome shotgun sequence.
ACCESSION   DASPGN010000347 DASPGN010000000
VERSION     DASPGN010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465547"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VD788_09955"
     CDS             complement(<1..810)
                     /locus_tag="VD788_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HXV76630.1"
                     /translation="MRHEDLPPAPAGGARLPLAIFDKCARYTQAREVQAAGLYPYFRT
                     ISDSEDTVVVMEGRRRLMLGSNNYLGLTHHPKVLEAAENALRRYGSGCTGSRFLNGTI
                     DLHCKLEAALAEFVGKQDCLVFSTGYMANLGVISGLVARRDVVYLDKLDHASIVDGAN
                     LSFGEIRRFNHGDLNDLERKLRNDVSVGGRLIMVDGVYSMEGNISDVPGLLLTARRFG
                     AALVVDDAHAIGVLGPRGNGTAAHYGLSNEVDVITGTFSKSLASIGGFVAAD"
     gene            1056..1685
                     /locus_tag="VD788_09960"
     CDS             1056..1685
                     /locus_tag="VD788_09960"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HXV76631.1"
                     /translation="MGENAETGRRTRAFASPWLERLSRIHPLVPLCVWAPLAVAAIVA
                     GARAGLSAAAVAGWTAAGLLAWTLTEYVLHRWLFHFEPADPGLRDKLYPVHRLHHDVQ
                     EWDRLVAPLLMSVPLAAVFFGLFSLVVGWPSALPLFGGFVIGYLAYDYIHFYTHFGRP
                     RTRIGRGLRRRHLQHHFACPDRWFGVSSPLWDYVFRTHVPRGSQARPRV"
     gene            complement(1682..3106)
                     /locus_tag="VD788_09965"
     CDS             complement(1682..3106)
                     /locus_tag="VD788_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76632.1"
                     /translation="MFKTVLLGMGAWLLAAAAAVAGPCDGAPAADEPRTLILALDGVP
                     FRVVRTVREMGAFEGWGETRPMVSPFPSMTNVGFAAILSPFGAPPIPGYELRRYDRER
                     NVVTGGGIRDSRFAWRKEFDLQQKGLWSKVGLYMAPRRNVTKEMRQLRRFLLDSTDRI
                     VTVLVSSTDPLTHFHGDAATARALLGVAEELELTRRVHRDVTGRELEIVMVSDHGNSP
                     HKVRRADGIKKSLRRAGLRPSKRLVRPDDVITVTYGVVGYGVLYLSPDRAEAAARSIV
                     DSEGVHLAAWRSADGEMRVRSSDGEARIRWRNDGPQRSFAYDVGSGDPLELGVAVERM
                     RRAGVVGTDGFARRDDWFEWTAFERYPDAVVRLVDSLGGAWVSNSATVIFSFEPGFAW
                     GIKAAEVGAWLRAGKLEETHGSLDRASSWGFFLASDPRLDASRAVSAEQALRPWAELS
                     RCRSTALVRLGTAGDGLHAPGHAR"
     gene            complement(3207..3590)
                     /locus_tag="VD788_09970"
     CDS             complement(3207..3590)
                     /locus_tag="VD788_09970"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXV76633.1"
                     /translation="MSSRPTSEHQVARPPFGAPHVFVLAVIDGAPPHRVHRIERSETV
                     IGREQGVDFLLDDQEISKRHCVLRADGPVCTVTDLGSLNGTRLNGRPLRDGVAQRLRH
                     LDQIQLGTTCLFFLAGKFRTPSVKD"
     gene            3692..>4011
                     /locus_tag="VD788_09975"
     CDS             3692..>4011
                     /locus_tag="VD788_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76634.1"
                     /translation="MNTRGFILQADYRVEAGAAVLLLYGKLEGGAGFLIRERRRRPWL
                     YVRRSQFARVADVDGGLCRVDETPPRVTMDGAPVVRLEHASPGDSATIRQRLERAGVE
                     TFEA"
BASE COUNT          610 a         1394 c         1375 g          632 t
ORIGIN      
        1 gtcggccgcg acgaagccgc cgatggacgc cagcgacttc gagaaggtcc cggtgatcac
       61 gtccacctcg ttcgacaggc cgtagtgtgc cgcggtgcca ttgccccgcg gcccgagcac
      121 tccgatcgca tgggcgtcgt cgacgacgag cgccgcgccg aatctccgcg cagttaagag
      181 caggccgggc acgtcgctga tgttgccttc catcgaatag acgccgtcga ccatgatcag
      241 ccggccgccg accgagacgt cgtttcgcag cttgcgctcg aggtcgttca ggtcgccgtg
      301 gttgaagcga cggatttcgc cgaagctcag gttggcgccg tcgacgatcg atgcgtgatc
      361 gagcttgtcg aggtagacca cgtcgcggcg ggcgaccaga ccggagatca caccgaggtt
      421 ggccatgtag cccgtcgaga agacgagaca atcctgcttc ccgacgaact cggcgagcgc
      481 cgcttcgagc ttgcagtgca ggtcgatcgt gccgttgagg aatcggctcc cggtgcaacc
      541 ggagccgtaa cgccgcaagg cgttctccgc cgcctccagc accttcgggt gatgcgtcag
      601 gccaaggtag ttgttcgagc ccagcatcaa ccgacgccgc ccctccatca cgacgacggt
      661 atcctcggaa tcggagatcg tgcggaagta ggggtagaga ccggcggcct gcacttctcg
      721 cgcctgcgtg tagcgcgcgc acttgtcgaa gatggcgagc ggcagccggg cgcctccggc
      781 gggcgcgggc ggcaggtcct cgtgacgcaa cctcgtccgg gcgctcggct cgacgaaggg
      841 gcgccggtat gtcggatcgg cgggagcgct gcgtctggaa acgtcgaacc aattccagcg
      901 ccaccgccgt gctactctgg tctcggacat ccggcctccg gagcggcggc ttccttgggg
      961 gaagtcggcc gaagcgggcg agccagtata acagtgccgg accggcgacc gcccgatcgt
     1021 ttcggggcgg ggcgtcacga ccggtcggag ggtggatggg agaaaacgcg gagaccggcc
     1081 gtcggacccg ggcgtttgcc agcccctggc tcgagcggct gagccggatc cacccgctcg
     1141 tcccgctctg cgtctgggca ccgctcgccg tcgccgcgat cgtggccggc gctcgcgcgg
     1201 ggctttcggc cgcggccgtc gccggctgga ccgccgccgg gctgctggcc tggaccttga
     1261 ccgaatacgt gctgcatcgc tggttgtttc atttcgagcc ggccgatccg gggttgcgcg
     1321 acaagctcta tccggtccac cgcctgcacc acgacgtgca agaatgggat cgcctggtcg
     1381 cacccctctt gatgtccgtc ccgctcgctg cggtgttctt cgggctgttc agcctcgtcg
     1441 tcggctggcc gtcggcctta ccgctgttcg gcggattcgt catcggctat ctggcttacg
     1501 actacatcca cttctacacc cacttcgggc gaccgcgcac gcggatcggc cgcggtctca
     1561 ggcgccgcca cctgcagcac catttcgcct gccccgatcg ttggttcggc gtcagctcgc
     1621 cgctgtggga ctacgtcttc cgcacgcacg tgccgcgcgg cagccaggcg aggccgcgcg
     1681 tctagcgcgc gtggcccgga gcgtggagtc cgtcgcccgc cgtgccgaga cggacgagcg
     1741 ccgtcgaccg gcagcggctc aactcggccc acgggcgcag cgcctgctct gcgctcaccg
     1801 cgcgcgaggc gtcgagtcgc gggtcgctcg ccaggaagaa gccccagctc gacgcccgat
     1861 cgagactccc gtgtgtttcc tcgagcttgc ccgcacggag ccacgcgccg acctcggcgg
     1921 ccttgatgcc ccaggcgaaa ccgggctcga acgaaaagat cacggtcgcc gagttgctga
     1981 cccacgcccc gccgagcgag tcgaccagcc ggaccaccgc gtccgggtag cgctcgaacg
     2041 ccgtccactc gaaccaatcg tcgcgccgcg cgaagccgtc ggtgccgacc acgccggcgc
     2101 gacgcattcg ctcgaccgcc acgccgagct cgagcgggtc tcccgagccg acgtcgtagg
     2161 cgaaggaccg ctgcggcccg tcgttccgcc agcggatgcg tgcctcgccg tcgctcgacc
     2221 ggacgcgcat ctcgccgtcg gcggaacgcc aggccgccag atggaccccc tcggagtcga
     2281 cgatcgaccg cgccgccgcc tccgcccgat ccgggctcag atacaggaca ccgtatccga
     2341 ccacgccgta cgtcacggtt atcacgtcgt ccggtcgaac gagccgcttc gacggacgca
     2401 ggccggcgcg acgcaatgac ttcttgatcc cgtctgcgcg acggaccttg tgcggggagt
     2461 tgccgtggtc ggagaccatc acgatttcga gctctcgtcc ggtgacgtcg cggtggaccc
     2521 gccgggtcag ctcgagctcc tcggccacgc cgagcaacgc gcgggccgtc gcggcgtcgc
     2581 cgtgaaagtg ggtcaacggg tcggtcgacg agacgagcac cgtgacgatc cggtcggtgg
     2641 agtcgaggag gaagcgccgc agctgccgca tctccttcgt cacgttccgg cgcggcgcca
     2701 tgtacaagcc gaccttggac cacaggccct tctgctgcag gtcgaactcc tttctccagg
     2761 cgaacctgga gtcgcgaatt ccgccgccgg tgacgacatt gcgctcccga tcgtagcgac
     2821 gcagctcgta tccgggaatc ggcggggcac cgaacgggct gagaatcgcc gcgaagccga
     2881 cgttcgtcat cgagggaaac ggcgagacca tcggccgagt ctcgccccaa ccctcgaacg
     2941 cgcccatctc gcgcacggtg cgcacgaccc ggaagggcac gccgtccagg gccaggatca
     3001 gcgtccgtgg ctcgtccgct gccggcgcgc catcgcacgg gcccgccacc gccgcggccg
     3061 ctgccagtaa ccaggcgccc atcccgagca gcaccgtctt gaacatcgac tggaaccccg
     3121 gcgcggtgtc cgcgcgcgtg acgcaacgcc cactccgccc gcgcaggtta gcacgcgcgg
     3181 attcccgtga tcagggggga gcgtgctcag tctttgactg acggcgtgcg gaacttgccg
     3241 gcgaggaaga acagacaggt cgtgccgagc tggatctgat cgagatgccg gagacgctgc
     3301 gccactccgt cgcgcagcgg acggccgttg agccgggtgc cgttgagact gcccagatcg
     3361 gtcacggtgc agacggggcc gtcggcccgc aagacgcagt ggcgcttgct gatctcctgg
     3421 tcgtcgagca agaagtcgac cccctgttcg cggccgatca ccgtttccga tcgctcgatc
     3481 cgatgcaccc ggtgcggcgg agcgccgtcg atcaccgcga gcacgaagac gtgcggcgcg
     3541 ccgaagggcg gacgggcaac ttggtgttcg ctcgtgggac gcgacgacat cggctctcct
     3601 tcggggtttc gcagagaatc tggcgatcgg gccgccggct gtcaagcgcg ggaaatcggc
     3661 ggtcggccgg gtgataatgc gatcggtgac ggtgaacaca cggggtttca tcctccaagc
     3721 ggactaccgc gtcgaggccg gggctgcggt cctgctgctg tacggcaagc tcgagggcgg
     3781 cgcgggtttt ctgatccgtg agcggcgccg tcggccgtgg ttgtacgtgc gccggtcgca
     3841 gttcgcgcgg gtcgccgacg tcgatggagg cctgtgccgg gtcgacgaaa cacccccgcg
     3901 cgtgacgatg gacggggcgc cggtggttcg tctcgagcac gcgtcacccg gtgattccgc
     3961 gacgatccgc cagcggctcg agcgtgccgg tgtcgagacg ttcgaagccg a
//
LOCUS       DASPGN010000348         4912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1465689, whole genome shotgun sequence.
ACCESSION   DASPGN010000348 DASPGN010000000
VERSION     DASPGN010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1465689"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VD788_09980"
     CDS             complement(<1..290)
                     /locus_tag="VD788_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HXV76635.1"
                     /translation="MAALAGSVDSRSPSGSDSRVDLPAYRSYGWRASPVSAAPATPPE
                     VEQAIDRELRRAGLERVGAEAELWVVVQLALDGPLAIRADELVYGGWPGWRG"
     gene            489..2558
                     /locus_tag="VD788_09985"
     CDS             489..2558
                     /locus_tag="VD788_09985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXV76636.1"
                     /translation="MSKTFRGLTVIAAATVLLAGCGGGGTEQPENPWTAENTPLIERE
                     VLFGNPERASVQISDDGSKIAWLAPVDDVLNVWVADADDPTAGSPVTNDTDRGIRIYF
                     WAPTNQHVVYVQDEGGDEDWQVYLTDVATRETRNLTPIDGVQARVAAVHHDFPNEIIV
                     ALNDRDPRHHDLYRVNLTTGERTLIEKNEDGFIEYTIDDDFNVRLATKVTQDGGAEVF
                     KRSLTGKWESYATIPSDDVLTTKPLGFDASGNVLYWIDSRGRDTAALLEVDLSSNASQ
                     VLGTSDKADVSDVMIHPTTSEVEAVAYTYDRKNWDVLDEAVAKDMEFLRTVTDGETEV
                     QDRTLDDRTWIVSYELDDGPVQYYLYDRDAGKADYLFSNRPALEELPLAEMHPVVIAA
                     RDGVQLVSYYTLPVWSDSTGDGIPEQPLPMALVVHGGPWARDDWGYNSLHQWLANRGY
                     AVLSVNFRGSTGLGKAHVNAGNLEWAGKMHDDLIDAVDWAVERGIAQRDKVAIMGGSY
                     GGYATLVGLTFTPDVFACGVDIVGPSNLNTLIESIPPYWEPMIELFAARVGDSRTETG
                     RTLLEERSPLRHADRIERPLLIGQGANDPRVKQAEADQIVAAMQTKEIPVTYVLYPDE
                     GHGFARPENRLSFFAVSEAFLAGHLGGRIEPVGDDFADSSIQVVEGAELIAGLTEALG
                     AQPEMAD"
     gene            complement(2621..4096)
                     /locus_tag="VD788_09990"
     CDS             complement(2621..4096)
                     /locus_tag="VD788_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HXV76637.1"
                     /translation="MIGATMATDRTFFGHPRGLAVLFFTELWERFGFYGMRALLILYL
                     TGQLGYDASEAGALYALYTSAVYLVGLPGGWLADRMLGQQRAVLWGGLLLAAGYLSLA
                     VPGLTAFYGGLAVIVVGTGLLKPNISAIVGKLYATGDGRRDAGFSIFYMGINVGALLG
                     PLVCSYLGENVDWRLGFAAAGVGMSCGVLVYLGWRSRLGQAGLHPSKPADTRRAALED
                     RRIKRVLAAAAVAVAGAVGLVVSGVVTVDVVAVSDYFGLVLALVTVAFFLWLFLGVRW
                     TPIERGRLAAIVVLFVAASIFWGAYEQAGSSLNLFADQSTDRSIPGSDRRFPAGWFQQ
                     APALFVILLAPVFAWLWVRLGPRQPSSPAKFALGLVFVGLGFVVMVGAAGAAASGRPV
                     SPAWLIGTYFLHVVGEMCLSPVGLGTVTKLAPERATGMMLGVWFLASAVGNYVGGRAA
                     GFYETFPLWKLFGLFAVVAIGAGLLLAWLTPPIRRLMGGIH"
     gene            4199..>4912
                     /locus_tag="VD788_09995"
     CDS             4199..>4912
                     /locus_tag="VD788_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV76638.1"
                     /translation="MTKKFDPIESFACMRHEFGEHGGVNMSIETSTTFTVMSADTLPE
                     IFQGRRGPTDDKGGCFLYGRHFNPTVYVLGKQLAALEGAAAGYCTASGMGAISSALVQ
                     LCDHGDHVVSSATVYGGTHALLNHFLPAKTGIGVTFVDVSDLDVVERAFTSRTRALYV
                     ESMANPTLRVADIPRLAELAHAHRAKLVVDNTFSPLIVSPIQLGADVVVYSLTKFING
                     ASDYIAGAICGSESFIMELM"
BASE COUNT          894 a         1699 c         1653 g          666 t
ORIGIN      
        1 cagccgcgcc agcccggcca gccgccgtag accagctcgt cggcccggat cgcgagcgga
       61 ccgtcgagcg cgagctgcac gacgacccac agctcggcct ccgccccgac ccgttccagt
      121 ccggcacggc gcagctcgcg gtcgatcgcc tgctcgacct cgggtggcgt cgccggggcg
      181 gcggaaaccg ggctcgcgcg ccagccgtag ctacggtagg ccggcaggtc gacgcgagaa
      241 tcgctgccgc tcggagaccg tgaatcgacg gacccggcga gggccgccaa cgcgacgaca
      301 accgccgccc tgcgcgggag cgacggtcgc tgccgcggga aaagtcgagc gtccgcgatc
      361 gcttttctcc tcgcagctcg ttccgccgca ttatagggcg atcgtcggcg cgactggcgg
      421 cggcgtggtc gcatgcgcta tggatagacg gcgaagttgc tttgcaaacg accatctgga
      481 gggtagagat gtcgaaaacg tttcgtggct tgacggtgat cgcggccgct accgtactgc
      541 tcgccggctg cggcggcggc gggaccgaac agcccgagaa tccctggacc gcggagaata
      601 cgccactgat cgagcgcgag gtgttgttcg gcaaccccga gcgagcgtcg gtgcagatca
      661 gcgatgacgg cagcaagatc gcgtggctcg cgccggtcga cgacgtgctg aacgtgtggg
      721 tcgcggacgc cgacgatccg acggcgggat cgcccgtaac caacgacacc gatcgcggaa
      781 tccgtatcta tttctgggcg ccgacgaacc aacacgtggt ctacgtccag gacgagggcg
      841 gcgacgagga ctggcaggtc tacctcaccg acgtcgccac ccgcgaaacg cgcaacctga
      901 cgccgatcga cggcgtgcag gcgcgggtcg ccgccgtcca ccacgacttc ccgaacgaga
      961 tcatcgtcgc gctcaacgat cgcgacccgc gtcaccacga tctgtaccgc gtcaacctga
     1021 cgaccggcga gcgcacgctg atcgagaaga acgaggacgg cttcatcgag tacacgatcg
     1081 atgacgactt caacgtgcgg ctggcgacca aggtgaccca ggacggcggt gccgaggtgt
     1141 tcaagcgctc gctgaccggt aagtgggagt cgtacgccac gatccccagc gacgacgtgc
     1201 tgacgacgaa gccgctcggc ttcgatgcca gcggcaacgt gctctactgg atcgacagcc
     1261 gcggtcgcga caccgccgcg ctgctcgagg tcgacctgtc gagcaacgcc tcccaagtgc
     1321 tgggtaccag cgacaaggcc gacgtcagcg acgtgatgat ccacccgacc acgagcgagg
     1381 tcgaggcggt cgcctacacc tacgatcgca agaactggga cgtgttggac gaggcggtgg
     1441 ccaaggacat ggagttcctg cggacggtga ccgacggcga gaccgaggtc caggatcgca
     1501 cgctcgacga ccgcacgtgg atcgtctcct acgagctcga cgacggtccg gtgcagtact
     1561 acctctacga ccgcgacgcg ggcaaggccg actacctgtt cagcaaccgc ccggcgctcg
     1621 aggagctgcc gctggccgag atgcacccgg tcgtgatcgc ggcgcgcgac ggcgtgcagc
     1681 tcgtcagcta ctacacgctg ccggtatggt cggactcgac cggcgacggc atccccgagc
     1741 agccgctgcc gatggcgctc gtcgtgcacg gtgggccgtg ggcgcgcgac gactggggct
     1801 acaactcgtt gcaccagtgg ctggccaacc ggggctacgc cgtgctcagc gtgaacttcc
     1861 gcggttcgac cggcctcggc aaagcccacg tcaacgccgg caacctcgaa tgggccggca
     1921 agatgcacga cgatctgatc gacgcggtcg actgggccgt cgaacggggc atcgcccagc
     1981 gggacaaggt cgcgatcatg ggtggcagct acggcggtta cgccacgctc gtcggtctga
     2041 ccttcacgcc cgacgtcttc gcctgcggcg tcgacatcgt cggtccgtcc aacctcaaca
     2101 cgctgatcga gtcgatcccg ccctactggg aaccgatgat cgagctgttc gccgcccggg
     2161 tgggtgacag ccgcaccgaa accggtcgca ccctgctcga ggagcgctcg ccgttgcgcc
     2221 atgccgaccg gatcgagcgc ccgctgttga tcgggcaggg cgccaacgac ccgcgcgtga
     2281 agcaggccga ggcggaccag atcgtcgcgg cgatgcagac caaggagatt ccggtgacct
     2341 atgtgctcta tccggacgaa ggacacgggt tcgcgcggcc ggagaatcgg ctgtcgttct
     2401 tcgccgtctc ggaggcgttc ctcgccgggc atctcggcgg acgcatcgag ccggtcggcg
     2461 acgacttcgc cgactcgagc atccaggtgg tcgagggagc cgagctgatc gccggtctga
     2521 ccgaggcgct cggcgcgcag cccgaaatgg ccgactgagc gcagttcgat tcggtgatgc
     2581 gagaacgccc ggggcggcga cgtcccgggc gttcgttcgt tcagtggatc ccgcccatca
     2641 gtcgccggat cggcggggtc agccaggcga gcagcaggcc cgcaccgatc gcgaccacgg
     2701 cgaacagacc gaacagcttc cacagcggga aggtctcgta gaagccggct gcgcgcccgc
     2761 cgacgtagtt gccgaccgcc gacgcgagaa accagacgcc gagcatcatg ccggtcgccc
     2821 gctcgggggc cagcttggtc accgtcccga gcccgaccgg actcagacac atctcgccga
     2881 ccacgtgcag gaagtaggtc ccgatcagcc aggccgggct gaccggccgg cccgaggcgg
     2941 ccgcgcccgc cgcaccgacc atcacgacga agccgagacc gacgaagacc agcccgagcg
     3001 cgaacttggc cgggctcgac ggctggcgcg gccccaaccg gacccacagc caggcgaaga
     3061 ccggcgcgag caggatgacg aacagcgccg gcgcctgctg gaaccagccg gcggggaacc
     3121 gtcggtcgga gccgggtatc gagcggtcgg tgctctggtc ggcgaacagg ttcaacgacg
     3181 agccggcttg ctcgtaggct ccccagaaga tcgaggcggc aacgaacaac acgacgatcg
     3241 ccgccagccg gccgcgttcg atcggcgtcc agcggacgcc gaggaacagc cagaggaaaa
     3301 acgcgaccgt cacgagcgcc agcaccagcc cgaagtagtc cgagaccgcg acgacgtcga
     3361 cggtcacgac cccggagacg accaggccga ccgcgccggc gacggcgacg gcggcggcgg
     3421 ccagcacccg cttgatccgg cggtcctcga gcgccgcgcg tcgcgtgtcc gccggcttgc
     3481 tcggatgtaa tcccgcctgg cccaaccgcg aacgccagcc gaggtagacc agcacgccgc
     3541 agctcatccc gaccccggcc gcggcgaagc ccagccgcca gtcgacgttc tcgcccaggt
     3601 agctgcagac cagcgggccg agcagcgcgc cgacgttgat ccccatgtag aagatcgaga
     3661 agcccgcatc gcgtcgcccg tcgcccgtcg cgtagagctt gccgacgatc gcgctgatgt
     3721 tgggcttgag cagacccgtg ccgacgacga tcaccgccag accgccgtag aacgcggtta
     3781 ggccgggtac cgccagcgac aggtagcccg cggccagcag cagcccgccc cacagcaccg
     3841 cacgctgttg gccgagcatg cggtcggcga gccagccacc gggcaacccg accaaataga
     3901 ccgccgaggt gtagagcgcg tagagcgcgc cggcctcgga ggcgtcgtac ccgagctggc
     3961 cggtgaggta caggatcaac aacgcgcgca tgccgtagaa gccgaaccgt tcccacagct
     4021 cggtgaagaa cagcacggcg agcccgcggg gatggccgaa gaatgtgcga tcggtcgcca
     4081 tcgtggcgcc gatcataacc tcgcccagcg cgaattgacg accccgacgg tcgtgattcg
     4141 atcgcgcgat ccgacttagg atggactgct gggtgcggga gcccgctcgg aggaccgaat
     4201 gaccaagaag ttcgacccga tcgagtcgtt cgcctgcatg agacacgagt tcggcgagca
     4261 cggcggcgtc aacatgtcga tcgagaccag cacgacgttc accgtgatga gcgccgacac
     4321 gctgcccgag atcttccagg gacgtcgcgg tccgaccgac gacaagggcg gctgctttct
     4381 ctacgggcga cacttcaacc cgacggtcta cgtgctcggc aagcagctcg cggcgctcga
     4441 gggtgccgcg gccggctact gcaccgcgag cggcatgggc gcgatctcga gcgcgctggt
     4501 gcagctgtgc gaccacggcg accacgtcgt ctcgtcggcg acggtctacg gtggcacgca
     4561 tgcgctgctc aaccatttcc taccggccaa gaccggcatc ggcgtgacct tcgtcgacgt
     4621 ttccgacctc gacgtggtcg agcgggcgtt cacctcgcgg acccgcgcgc tgtacgtcga
     4681 gagcatggcc aatccgacgc tgcgcgtcgc cgacataccg cggctcgccg aactggccca
     4741 cgcccaccgc gccaagctgg tagtcgacaa caccttcagc ccgctgatcg tctcgccgat
     4801 ccagctcggg gcggacgtcg tcgtctacag cctgaccaag ttcatcaacg gcgcgtcgga
     4861 ctatatcgcc ggcgcgatct gtggcagcga gtcgttcatc atggagctga tg
//
LOCUS       DASPGN010000349         6661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_529784, whole genome shotgun sequence.
ACCESSION   DASPGN010000349 DASPGN010000000
VERSION     DASPGN010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529784"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VD788_10000"
     CDS             complement(<1..929)
                     /locus_tag="VD788_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76639.1"
                     /translation="MTIRQPEDLASANAEPIPIDAPQAFANRELSWLAFARRVLFMVE
                     DDELPLLERAKFVGIMGMLHYEFYSKRVSGLKRQIKEKPNRLSVDGRTPAQELADCRA
                     EVVRQVEVLNRVVETEILPGLAAAGLPILRWHRLRPEQRTRMLHYFERSVRPILIPLA
                     VDAEHPFPFISNLGLNLAALVPDPQGGPHRFVRTKVPVNRPRWVPLGDGEGWVPLEEI
                     IKANLHRLFPGAPPVSVYAFRVTRGAEGSSRVAADATDTEVFEEPGSIVRQVSAELKA
                     RRFAGVVRLQVEQAMPADLRKWLGEQLGVDAGDV"
     gene            1085..2326
                     /gene="glgA"
                     /locus_tag="VD788_10005"
     CDS             1085..2326
                     /gene="glgA"
                     /locus_tag="VD788_10005"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HXV76640.1"
                     /translation="MAGLMRVVFMAREYPPEVYGGAGVHLEHLARAMARLARVEVKCF
                     GERPADAAGGLRVVGYPYGDSIFADNPDPARHALAALETCLRFNGLPVEADVVHCHTW
                     YSHFGGLLAKIVYGTPLVVTVHSLEPLRPWKRDKLGRGYDLSLWVERTTLELADAVIA
                     VSAPERAQVLDRFEVRPDRVHVIPNGVDTDVYRKIDGKPALRRLGIDPDRPFVLFVGR
                     LSRQKGIGHLLRAVRLLPPSIGLALVAASPDSNAIEREVEAAVDELRRERDNVSWIRR
                     MLPRDETIALYSEAAVFCCPSVYEPFGIINLEAMACETPVVGTAVGGIRETVVPEQTG
                     LLVGFEPRSADDPEPRDPQRLARELAAAIERAVTDETWRAEAGRRGRRRVVERYGWDR
                     VAARVFDVYRRAVAGTGGGMR"
     gene            2323..4308
                     /locus_tag="VD788_10010"
     CDS             2323..4308
                     /locus_tag="VD788_10010"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HXV76641.1"
                     /translation="MTTTKTDLPDELARRVWVCNVRPRVGDGDLPIKRTVGETVAVTA
                     DLFCDGHDKLAAVVRHKARSAAEWREVAMAPEPNDVWRAEFRVDDQEPHQFFVEAWVD
                     HFSTWRAALEKKAAAGQDVGSELLEGAALVDAASARARGEDRSRLVEAAGRLAGDRPQ
                     AERIEQARADELGAWMARHDERRRATRSEPYTVEVSRARARFGAWYEFFPRSVGSGTL
                     RDAAGRLPDIARMGFDVVYLPPIHPIGRTQRKGPNNTLEATADAPGSPWAIGSEAGGH
                     TAVHPDLGGLDDFDHFRREAERLGLEVALDVAFQCSPDHPWVLDHPQWFKRRPDGTIK
                     HAENPPKSYQDIYPLDFDTDDWRALWYELLGVVAFWVDRGVRIFRVDNPHTKPIRFWG
                     WLISEIKRRHPDTIFLSEAFTRPKVMYALAQVGFDQSYTYFTWRNSKQDLIDYSSQLT
                     RTEVRDFLRPNLWPNTPDILPEYLQIGGRPAFMSRLVLAATLGASYGIYAGYELCENE
                     SLAGREEYLNSEKFEIKQRDWDWPGNLRGYIARVNRIRRENPALAYDHRLRFHDTDNE
                     QLLVYSKTSEDLSNVIVVAVNLDPHHVHHGWVELSLDELGIDRDQDYQVHDLLGQGRY
                     LWRGPRNYLRLDPESSPAQIFRVRRRLRTEQDFDYFS"
     gene            4331..>6661
                     /gene="treS"
                     /locus_tag="VD788_10015"
     CDS             4331..>6661
                     /gene="treS"
                     /locus_tag="VD788_10015"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HXV76642.1"
                     /translation="MPADKDDPLWYKDAIIYELHVKAFQDSNHDGRGDFRGLIQRLDY
                     LQDLGVTAVWLLPFYPSPLRDDGYDISDYRGIHPDYGTLRDFRALIREAHQRGLRVIT
                     ELVVNHTSDQHRWFQEARRARAGSSRREFYVWSDSDTRFAGTRVIFTDNERSNWAWDP
                     VAGAYYWHRFFSHQPDLNFDNPQVRRAIKGVMRYWLDMGVDGLRLDAVPYLVERDGTN
                     NENLPETHEVIKELRAEVDRHYTGRMLLAEANQWPDDVRPYFGDGDECHMAFHFPLMP
                     RIFMAQRQEDRRPIVDILQNTPAIPEVCQWALFLRNHDELTLEMVTDEERDYMYKEYA
                     TDPRMKVNVGIRRRLAPLLDNGRRRIEMLNSLLFSFPGTPIVYYGDELGMGDNIFLGD
                     RDGVRTPMQWTADRNAGFSRCDPARLYLPLIMDAVYGYHAVNVEAQQRNPSSLLQFMK
                     RLISLRRQHKAFGRGTIDFLHPRNRRILAYLRRWRGEVILCVANLSRYVQPAELDMRE
                     FDGWTPVEMIGRTEFPPIGQLPYFLTLAPHAFYWFQLEPQPQPIEMLSRGEAAGALPS
                     LTLEEGYDQLFAPEYRVTLLEEVIRPFLGRQRWFQGKAREIVRLQLVDWVKLGGGFYV
                     ALADVHYADERCECYVLPLKIARGEPARRALAEAASGVLARVRTAEGEGVIYDALADH
                     HATASLLNAMADGRDYRTAMRNRLRAYVTRAVEAVGRQVSSVRRLSAEQSNTSVIVDD
                     AFILKLFRRVEPGPSPELELGRFLTERTGFRNLAAVA"
BASE COUNT         1028 a         2303 c         2294 g         1036 t
ORIGIN      
        1 acgtcgccgg cgtcgacgcc gagctgctcg ccgagccact tgcgcagatc ggccggcatc
       61 gcctgctcga cctgcagccg cacgacgccg gcgaagcgcc gggccttcag ctcggcgctg
      121 acctggcgca cgatgctgcc cggttcctcg aacacctcgg tgtcggtcgc gtcggcagcg
      181 acccggctcg acccctcggc accgcgggtg acgcggaacg cgtaaaccga taccggcggt
      241 gcgccgggga acagccggtg caggttggcc ttgatgatct cctcgagcgg aacccatccc
      301 tcgccgtccc ccagcggcac ccagcgtggc cgattgaccg gcaccttggt ccggacgaag
      361 cgatgggggc cgccctgggg atcgggcacg agtgcggcca ggttgagccc caggttgctg
      421 atgaacggaa acgggtgctc ggcgtcgacg gcgagcggga tgaggatcgg ccgcaccgag
      481 cgctcgaagt agtgcagcat gcgggtccgc tgctcgggcc gcagccggtg ccagcgcagg
      541 atcggcaggc cggctgcggc caacccgggc aggatctcgg tctcgaccac gcggttcaac
      601 acctcgacct gccgcacgac ctcggcgcga caatccgcca gctcctgggc cggcgtccgc
      661 ccgtcgacgg acagcctgtt cggcttttct ttgatctgac gcttcagccc gctcacccgc
      721 ttgctgtaga actcgtagtg cagcatgccc atgatgccga cgaacttggc gcgctcgagc
      781 agcgggagct cgtcatcttc caccatgaac agcacgcgcc gtgcgaacgc gagccacgac
      841 agctcacggt tggcgaacgc ctgcggggcg tcgatcggga tcggttcggc gttcgcggac
      901 gccaggtcct cgggctgtcg aatcgtcatc tctttgcggg cctcgtagga acagtctagt
      961 gcctcggcgg ccgggtgtga agcaccggcc cccgtcgccg ggtaagatct cccccgatgg
     1021 gcaggcgatg cggttctcgg gagactgcgc agtggggcac gcgccggttt tgcacgaggg
     1081 ggacgtggca ggtttgatgc gtgtcgtctt catggcgcgg gagtatccgc ccgaggtcta
     1141 cggcggggcc ggcgtgcacc tcgagcacct ggcccgcgcg atggcccgcc tcgcccgggt
     1201 cgaggtcaag tgcttcggcg agcgcccggc ggacgccgcg ggcgggctgc gggtcgtcgg
     1261 ctacccgtac ggcgattcga tcttcgccga caatcccgac cccgcccgtc acgcgctggc
     1321 ggcgctcgag acctgcctgc gcttcaacgg cctgcccgtc gaggccgacg tggtgcactg
     1381 ccacacgtgg tacagccact tcggcgggct gttggcgaag atcgtctacg gcacgccgct
     1441 cgtcgtcacg gtccattcgc tcgagccgct gcggccgtgg aagcgcgaca agctcggccg
     1501 tggatacgac ctgtcgctgt gggtcgaacg cacgacgctc gagctggcgg acgcggtgat
     1561 cgcggtttcc gcgcccgagc gtgcgcaggt cctcgatcgt ttcgaggtgc ggccggaccg
     1621 cgtgcacgtg atcccgaacg gtgtcgacac cgacgtctat cgcaagatcg acggcaagcc
     1681 ggcgctgcgg cgcctcggga tcgatcccga ccgcccgttc gtgctgttcg tcggacgtct
     1741 cagccggcag aaggggatcg ggcatctgtt gcgcgcggtg cggctgttgc cgccgtcgat
     1801 cggcttggcg ctcgtcgccg cctcgcccga ttcgaacgcg atcgagcgcg aggtcgaagc
     1861 ggcggtcgac gagctgcggc gcgagcgcga caacgtgagc tggatccgcc gcatgctgcc
     1921 gcgggacgag acgatcgcgc tctattccga ggccgccgtc ttctgctgcc cgtcggtcta
     1981 cgagccgttc ggtatcatca atctcgaggc gatggcgtgc gagacgccgg tcgtcggcac
     2041 ggcggtcggt gggatccgcg agacggtggt gccggagcag accgggctgc tcgtcgggtt
     2101 cgagccccgc tcggccgacg atccggagcc gcgcgacccg cagcgtctgg cgcgcgagct
     2161 ggccgccgcg atcgagcgcg ccgtcaccga cgaaacgtgg cgtgccgagg cgggacgccg
     2221 cggtcgccgg cgcgtggtcg aacgatacgg ttgggaccgg gtggccgctc gcgtcttcga
     2281 cgtctatcgc cgcgccgttg ccgggaccgg cggagggatg cgatgacgac gacgaagacg
     2341 gacctgccgg acgagctggc gcgccgcgtc tgggtctgca acgtgcgacc gcgcgtcggc
     2401 gacggcgacc tgccgatcaa gcgcacggtc ggcgagacgg tggcggtcac ggccgacctc
     2461 ttctgcgacg gtcacgacaa gctcgcggcc gtcgttcgcc acaaggcccg gtcggccgcc
     2521 gagtggcgcg aggtggcgat ggcgcccgag ccgaacgacg tatggcgagc cgagttccgt
     2581 gtcgacgatc aggaacccca ccagttcttc gtcgaggcgt gggtcgatca cttctcgacg
     2641 tggcgcgccg cgctggagaa gaaggcggcg gccggccagg acgtcggcag cgagctgctc
     2701 gagggtgccg cgttggtcga cgcggcgagc gctcgcgcgc gcggtgagga ccgcagtcgc
     2761 ctggtcgagg cggccgggcg gctcgccggc gatcggccgc aggcggagcg gatcgaacag
     2821 gcgcgcgccg acgagctcgg agcgtggatg gcgcgccacg acgagcggcg gcgcgcgacg
     2881 cgctccgagc cgtacacggt cgaggtgtcc cgcgcgcgcg cgcgcttcgg cgcatggtac
     2941 gagttcttcc cgcgctcggt cggctcgggc acgctgcgcg atgccgcggg acgcttgccc
     3001 gacatcgcgc ggatggggtt cgacgtcgtc tacctgccgc cgatccaccc gatcggccgg
     3061 acgcagcgca agggcccgaa caacacgctc gaggcgacgg ccgacgcccc gggcagcccg
     3121 tgggcgatcg gctccgaggc gggcggccac accgcggtgc accccgacct gggcggactc
     3181 gacgacttcg accacttccg ccgcgaggcg gagcggctcg gcctggaagt cgcgctcgac
     3241 gtcgccttcc agtgctcgcc cgaccatccg tgggtcctcg accacccgca gtggttcaag
     3301 cgccggccgg acgggacgat caagcacgcc gaaaacccgc cgaagagtta ccaggacatc
     3361 tatccgctcg actttgacac cgacgactgg cgcgcgctgt ggtacgagct gctcggcgtc
     3421 gtcgccttct gggtcgaccg tggcgtgcgc atcttccgcg tcgacaaccc gcataccaag
     3481 ccgatccggt tctggggttg gctgatctcc gagatcaagc gccgtcaccc cgacacgatc
     3541 ttcctgtcgg aggcattcac ccggcccaag gtgatgtacg cgctggccca ggtcggcttc
     3601 gatcagtcct acacctactt tacctggcgc aacagcaagc aagacctgat cgactactcg
     3661 agtcagctga cgcggaccga ggtgcgggac tttctgcgtc ccaacctgtg gcccaacacg
     3721 cccgacatcc tgcccgagta cctgcagatc ggcgggcgcc cggcgttcat gagccggctg
     3781 gtgctggccg cgacgctcgg cgcgagctac ggcatctacg ccggctacga gctgtgcgag
     3841 aacgagtcgt tggcgggccg cgaggagtac ttgaactcgg aaaagttcga gatcaagcag
     3901 cgcgactggg actggccggg caacctgcgg ggctacatcg cgcgtgtcaa ccgcatccgg
     3961 cgcgagaacc cggcgctggc ctacgatcat cggctgcggt tccacgacac cgacaacgag
     4021 cagctgttgg tctacagcaa gacgagcgag gatctgtcga acgtgatcgt cgtcgccgtc
     4081 aacctcgacc cgcatcacgt gcaccacggc tgggtcgagc tgtcgctcga cgagctgggt
     4141 atcgatcgcg atcaggacta ccaggttcac gacctgctgg gccagggccg ctacctctgg
     4201 cgcggcccgc gcaactacct ccgcctcgac ccggaatcgt caccggcgca gatcttccgc
     4261 gtcaggcgcc gcttgcgcac cgagcaggac ttcgactact tcagttgagc gacgagacga
     4321 ggaaccgacg atgcccgccg acaaggacga tccactctgg tacaaggacg cgatcatcta
     4381 cgagctgcac gtcaaggcgt tccaggacag caaccacgac ggtcggggcg attttcgcgg
     4441 tctgatccaa cggctcgact acctgcagga cctgggcgtg accgcggtct ggttgctccc
     4501 gttctatccg tcaccgctgc gcgacgacgg ctacgacatc tccgactatc gcgggatcca
     4561 cccggactac ggcacgctgc gcgacttccg cgcgttgatc cgcgaggcgc accagcgcgg
     4621 cctccgcgtg atcaccgagc tcgtcgtcaa ccacacctcc gaccagcatc gctggtttca
     4681 ggaggcccgc cgcgcgcggg ccggcagctc gcgtcgcgag ttctacgtct ggagcgacag
     4741 cgacacccgc ttcgccggaa cgcgcgtcat cttcaccgac aacgaacggt cgaactgggc
     4801 gtgggacccc gtcgccggcg cctactactg gcatcgcttc ttttcgcacc aaccggacct
     4861 caacttcgac aacccgcagg tccggcgcgc gatcaagggc gtaatgcggt actggctcga
     4921 catgggggtc gacggcttgc gcctcgacgc cgtgccgtac ctggtcgagc gcgacgggac
     4981 gaacaacgag aacctgcccg aaacccacga ggtgatcaag gagctgcggg ccgaggtcga
     5041 ccggcattac accggccgca tgctgctggc cgaagccaac cagtggccgg acgacgtgcg
     5101 cccgtacttc ggcgacggtg acgagtgcca catggccttc catttcccgc tgatgccccg
     5161 gatcttcatg gcgcagcgcc aggaggatcg gcgcccgatc gtcgacatcc tgcagaacac
     5221 gccggcgata cccgaggtct gccagtgggc gctgttcctc cgcaaccacg acgagctgac
     5281 gctcgagatg gtgaccgacg aggaacggga ctacatgtac aaggagtacg cgaccgaccc
     5341 gcggatgaag gtcaacgtcg gcatccgccg ccggctcgcg ccgctgctcg acaacggccg
     5401 gcggcggatc gagatgctga acagcctgtt gttctcgttc cccggcacgc cgatcgtgta
     5461 ctacggcgac gagctgggca tgggcgacaa catcttcctc ggcgaccgcg acggcgtgcg
     5521 cacgccgatg cagtggacgg cggatcgcaa cgccggcttc tcccgctgcg acccggcgag
     5581 gctgtacctg ccgttgatca tggacgcggt ctacggctat cacgccgtca acgtcgaggc
     5641 ccagcagcgc aatccgtcgt cgctgctgca gttcatgaag cgcttgatct cgctgcgccg
     5701 ccagcacaag gcgttcggcc gcgggacgat cgacttcctc catccgcgca accggcggat
     5761 cctggcctat cttcgtcgct ggcgcggcga ggtgatcctg tgcgtcgcca acctgtcgcg
     5821 ctacgtgcag cccgccgagc tcgacatgcg cgagttcgac ggctggacgc cggtcgagat
     5881 gatcggtcgt accgagttcc cgccgatcgg ccagcttccc tacttcctca ccctcgcacc
     5941 gcacgccttc tactggttcc agctcgagcc gcagccacag ccgatcgaga tgctgtcgcg
     6001 tggtgaggcg gccggcgcgc tgccgtcgct gacgctcgag gaaggatacg atcaactgtt
     6061 cgcgcccgag taccgcgtca cgctgctcga ggaggtgatc cgaccgtttc tcgggcggca
     6121 gcgctggttc cagggcaagg cgcgggagat cgtccgtctg cagctcgtcg actgggtcaa
     6181 gctcggcggc ggcttctacg tcgccttggc cgacgtgcac tacgcggacg aaaggtgcga
     6241 gtgctacgtc ctgccgctca agatcgcgcg cggcgagccg gcgcggcgcg cgctggccga
     6301 ggcggcgtcc ggcgtgctgg cgcgggttcg cacggccgag ggggagggcg tgatctacga
     6361 cgccctcgcc gatcaccacg cgacggcgtc gctgctcaac gcgatggccg acggtcgcga
     6421 ctaccgaacg gcgatgcgca accggctgcg ggcctacgtc acgcgcgccg tcgaggcggt
     6481 cgggcggcaa gtgtcgtcgg tgcgccgtct gtccgccgag cagagcaaca cctcggtgat
     6541 cgtcgacgac gcgttcatcc tcaagctgtt ccgccgcgtc gagccggggc cgagccccga
     6601 gctcgagctg gggcgctttc tcaccgagcg cacgggcttt cgcaacctgg cggcggtggc
     6661 c
//
LOCUS       DASPGN010000350         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_413682, whole genome shotgun sequence.
ACCESSION   DASPGN010000350 DASPGN010000000
VERSION     DASPGN010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413682"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VD788_10020"
     CDS             <1..1210
                     /locus_tag="VD788_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76643.1"
                     /translation="TLSGDGFYDLGNDFAFGEWRDSLPREIEGAPFERHVEAGLGNLR
                     RIYRSIAAGFGGKIDSGLSGGYDSRLTLALLREQGVTPALHVYGKSDDPDVRVATEIA
                     RGEGLTLEHVDKSMRPATSAEDFPAVVADNCHALDGCPPDGIFDGGADVETRRERCAG
                     GELMLNGGGGEIFRNFFYLPDRSLTVRAFLWSFYSRYDPSWCTDRFSEEAYLTRLGDK
                     ITADLGTTGGKLRRAEIELIYPLFRCRYWMGKNNSNNNRFGWALTPFIDYRTVGGAVR
                     VPIDYKSYGRLQAELIRRIDPRLAGYPSVYGHPFAAPPPLGRRVKELLSIARPPLLRR
                     YLYRIKYRRPQPRGRYLDDRFVHRVVDPGFPFLSRFFKLDRINDPAVYNRVCTLEYLF
                     ERHDPSDAPV"
     gene            complement(1223..2704)
                     /locus_tag="VD788_10025"
     CDS             complement(1223..2704)
                     /locus_tag="VD788_10025"
                     /inference="COORDINATES: protein motif:HMM:NF045042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXV76644.1"
                     /translation="MGISLGRRRLVLTLFALYLAFVHLMLGLALWKSDFVGLLLKNLT
                     PILEPLLDRGVDYSTIEPLVIPLAELDGWDDTGGVVAADLDGDGRREFVVTGRAGVAA
                     FGSDGARLWFHAVDLQLTWKSEVHGLPGRHAPGVAVADVDGDSSTELMLLTRDGRLVV
                     IDGVAGAVERTVRLPVPADAERWEHLVLAHFRRSDELDLLLQATDRPYFRSGRFLAAY
                     DLERLLAGEPTTPLWATDRFRGAGHSGVRVADLDGDGLDEVVGAMILGPDGGVRVEIP
                     VRVDAKSHVDALHVADVRPDLPGLEVVALEESPENRIFLYNHRGVIWVAHHRNKEAQF
                     AAIGDFDTTRPGLEVWSRSRMIKHQTPWIFDSRGRLIASYWMNFVTPPDWTVEGMAVP
                     TVIDWTGSAEQLVAAMEFGTAGDVAVFDPVTGRLLHRFEERAERLYVADVQGDWREEL
                     IVASGGELHVYSNDQPNPRPEREPLWNEPQYVRRKINWNSYNP"
     gene            complement(2721..>3694)
                     /locus_tag="VD788_10030"
     CDS             complement(2721..>3694)
                     /locus_tag="VD788_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HXV76645.1"
                     /translation="TEHHEYYLEPADVVALIPRVAEIYSEPFGNESVVPAYHCARMAN
                     ELGIRVLLGGDGGDELFAGNERYATQKIFDLYRRVPSPLRRGLLEPVVTHFPAGGSIW
                     PVRKARGYIAKARMRMPDRTQIYNYMERFGADTVFQPDFLAEVDVDEPRRLLRESYDG
                     VRADSMLNRCLAMDLRFTLADNDLPKVSRMAELEGVRVCYPLLDDELIDFAARLPPEL
                     KLRRLELRWFFKHALRDFLPEEIIRKTKHGMGMPFGLWLETYAPLQELVRDSLDALKR
                     RDIVRPAFVDQVSEMHRNEHAVYYGVMIWLLVQLEQWLRCHDDGSGV"
BASE COUNT          623 a         1257 c         1208 g          606 t
ORIGIN      
        1 gacattgagc ggcgacggat tctacgacct cggcaacgac ttcgcgttcg gcgagtggcg
       61 agactctctt ccgcgagaaa tcgagggtgc gccgttcgag cgacacgtcg aggccggcct
      121 cggtaacctg agacgcatct atcggagcat cgcggcgggc tttggcggca agatcgactc
      181 gggcttgtcc ggcgggtacg attcacgctt gaccctggcc ctgttgcgcg aacagggcgt
      241 cacgcccgcc ctgcacgtct atggcaagag tgacgatccg gacgtccgcg tcgcgaccga
      301 aatcgcgcgg ggagagggtc tgaccctcga gcacgtcgac aagtcgatgc gtcccgcgac
      361 ctcggccgag gactttccgg cggtcgtcgc ggacaactgt cacgcgctcg acggttgtcc
      421 gcccgacggg atcttcgacg gaggagcgga cgtcgagacg cgacgggagc gttgtgccgg
      481 cggcgagttg atgttgaacg gtggtggcgg ggagattttt cgaaacttct tctaccttcc
      541 cgatcgttcg ttgaccgttc gggcgtttct ctggtctttc tacagtcgct acgatccgag
      601 ctggtgcacc gaccgcttct cggaggaggc gtacctgacc cgtctcggcg acaagatcac
      661 ggccgatctg ggtacgaccg gcggcaagct gcggcgggcg gagatcgagc tgatctaccc
      721 gttgttccgc tgtcgttact ggatggggaa gaacaacagc aacaacaacc ggttcggctg
      781 ggcgctgacg ccgttcatcg attaccgaac ggtcggcggg gcggtccggg ttccgatcga
      841 ctacaagagc tacgggcgcc tccaggccga gctgatccgg cgcatcgacc cgcgactggc
      901 cggctatccg tcggtctacg gccatccctt cgcggccccg ccgccgctcg ggcggcgggt
      961 caaagagctg ctgtcgatcg ccaggccgcc gctgcttcga cgctatctct accggatcaa
     1021 gtatcgacga ccgcaaccgc gcggaaggta cctcgacgac cgcttcgtcc atcgcgtcgt
     1081 cgaccccggt tttccgttcc tgtcccgctt cttcaagctc gaccggatca acgacccggc
     1141 ggtctacaac cgggtctgca cgctcgagta cctgttcgaa cgccacgatc ccagcgacgc
     1201 gccggtctag cacgaccgcc gctcacgggt tgtacgagtt ccagttgatc ttgcgacgga
     1261 cgtactgagg ctcgttccac agcggttcgc gctcggggcg cgggttcggc tgatcgttcg
     1321 agtagacgtg cagctcgccg ccgctcgcga cgatcaactc ctcgcgccag tcgccctgaa
     1381 cgtcggccac gtacagccgc tcggcccgtt cctcgaaccg gtgaagcagc cgtcccgtga
     1441 ccggatcgaa cacggccacg tcgcccgccg tgccgaactc catcgcggcc accagctgct
     1501 cggcggagcc ggtccagtcg atcaccgtcg gcaccgccat tccctcgacc gtccagtcgg
     1561 ggggcgtgac gaagttcatc cagtagctcg cgatgaggcg gcctcgcgaa tcgaagatcc
     1621 agggcgtctg gtgcttgatc atccgactgc ggctccagac ctcgagtccc ggccgggtgg
     1681 tatcgaaatc accgatcgcg gcgaactgcg cctctttgtt tcggtggtgg gcgacccaga
     1741 tgactccccg gtggttgtaa aggaagatcc gattctcggg cgactcctcg agcgcgacca
     1801 cctcgaggcc cggaagatcc ggtcgcacgt cggcgacgtg cagcgcgtcg acgtgcgatt
     1861 tcgcgtcgac gcggaccggg atctccaccc gcacgccacc gtccgggccg aggatcatcg
     1921 cccccaccac ctcgtccaac ccgtcgccat cgagatccgc gacgcgaacg ccgctgtgac
     1981 cggcaccgcg gaaccggtcg gtcgcccaga gcggggtggt cggctcgccg gccagcagcc
     2041 gttcgaggtc gtaggccgcg aggaagcggc ccgagcggaa gtaaggccga tcggtcgcct
     2101 ggagcaacag gtcgagctcg tccgatcggc ggaagtgcgc gagcacgagg tgctcccagc
     2161 gctcggcatc cgccggcacc ggaagccgca cggtccgctc gaccgctccg gcgacgccat
     2221 cgatcacgac caggcgaccg tcacgcgtga ggagcatcag ctcggtcgac gagtcgccgt
     2281 cgacgtcggc gaccgcgacc ccgggcgcgt gccgcccggg gagtccgtgg acttcggact
     2341 tccacgtcag ctggagatcg accgcgtgga accacagccg cgcgccgtcc gaaccgaacg
     2401 ccgcgacgcc ggcccggccg gtgacgacga actcgcgccg gccgtcgcca tcgaggtcgg
     2461 cggcgaccac ccctccggtg tcatcccatc cgtccagctc agcgagcgga atcaccagcg
     2521 gctcgatcgt cgagtagtcg acgccacgat cgagcagcgg ctcgaggatc ggcgtcaggt
     2581 tcttcagcaa cagtccgaca aagtccgact tccacagcgc gagaccgagc atcaggtgga
     2641 cgaacgcgag gtacagcgcg aacaacgtca acacgagtcg gcgccggccg agcgagatcc
     2701 ccacgtgcga ccccttctcc tcagacgccc gaaccgtcgt cgtggcatcg cagccactgt
     2761 tcgagctgaa ccaacagcca gatcatcacg ccatagtaaa cggcgtgctc gttgcggtgc
     2821 atctcgctca cctggtcgac gaacgccggg cggacgatgt cgcgccgttt cagagcgtcc
     2881 aggctgtccc gcaccagttc ctgcagcgga gcataggtct cgagccacag gccgaagggc
     2941 atgcccatgc cgtgcttggt cttgcggatg atctcctccg ggagaaaatc ccgcaacgcg
     3001 tgcttgaaga accagcgcag ctcgagccgg cgcagcttca gctcgggcgg caggcgggcg
     3061 gcgaagtcga tcagctcgtc gtcgagcaac gggtagcaga cgcgtacgcc ctcgagctcg
     3121 gccatccggc tgactttcgg cagatcgttg tcggccaagg tgaagcgcag gtccatcgcc
     3181 aggcagcgat tgagcatgct gtcggcgcgc acgccgtcgt agctctcacg cagcagccgt
     3241 ctcggctcgt cgacgtcgac ctcggcgagg aagtccggct ggaacaccgt gtcggctccg
     3301 aatcgttcca tgtagttgta gatctgcgtc cggtccggca tccgcatccg cgccttcgcg
     3361 atgtagccgc gcgccttccg cacgggccag atcgatccac cggcggggaa gtgcgtgacg
     3421 accggctcga gcagcccgcg gcgcaacggc gacggaaccc gccggtagag gtcgaagatc
     3481 ttctgcgtgg cgtatcgctc gttgccggcg aacagctcgt cgccgccgtc gccgccgaga
     3541 agcacgcgga tgccgagctc gttcgccatc cgcgcgcagt ggtaggccgg cacgaccgat
     3601 tcgttgccga acggctcgga gtagatctcg gcgacgcgag ggatcagcgc gacgacgtcg
     3661 gcgggctcga gatagtactc gtggtgctcg gtgc
//
LOCUS       DASPGN010000351         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1584436, whole genome shotgun sequence.
ACCESSION   DASPGN010000351 DASPGN010000000
VERSION     DASPGN010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1584436"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1585)
                     /gene="trpC"
                     /locus_tag="VD788_10035"
     CDS             complement(149..1585)
                     /gene="trpC"
                     /locus_tag="VD788_10035"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HXV76646.1"
                     /translation="MRCDASRGCALRDVLQRIVRAKRADVARRRLACPLDATATARRE
                     PIRSFAAALGRDGLSLIAEFKRRSPSSGAIRIDADPALRAIDYQRGGAAAISVVTDRE
                     FFAGCDDDLIRARSASTLPVLRKEFIVDPYQIHETAALGADALLLIVRILDDRSLARA
                     IRTTRELAIEPLVEVHDEHDLARAVDAGATIVGLNSRDLDTFRVDLSRTISLRATVPA
                     GLIAVAESGVETAEDARRVREAGFDAVLVGEALMRADDPRQLIEAWTGRRMEPRRSWV
                     KICGVTELDDALDAVRAGADAIGFVFARSPRRIEPARARRIIERLPADVRTVGVFVDE
                     SHDRLVRTVRESGVDMIQLHGDEPPSLCGRLTRPVIKRIRVGRTDDGETLLARMRRYD
                     VAAFLLDPGAGNGRPFDWSIARRLGQRVVVAGGLSAANVPRALSGADPYGVDACSGVE
                     RYPGRKDPDKLHAFVRAVRQADERDRTR"
     gene            complement(1548..>2430)
                     /gene="trpD"
                     /locus_tag="VD788_10040"
     CDS             complement(1548..>2430)
                     /gene="trpD"
                     /locus_tag="VD788_10040"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HXV76647.1"
                     /translation="KGETVAEITGFARAMRDAAEPLRHPFDDAIDTCGTGGDGRSTFN
                     VSTVSAFVAAGAGCRVAKHGNRSVSSRCGSAEALEALGIDPRASARLTARLLAEVGVG
                     FLFAPDYHRAMRHAVEPRREIGLRTIFNIVGPLTNPAGVRRQLVGVFERELTEVVAKV
                     LARLGSTHCVVVHGADGLDEITLGGPTHVCEWRDGTLDRYRLDPRRFGFARVAAGRLR
                     GGSPRENARIALAVLEGRPGPARDVALINAGAAIYVGGVAESIETGIERARESIDSGA
                     AVEKLHALRRFAGLRVA"
BASE COUNT          354 a          899 c          856 g          321 t
ORIGIN      
        1 cccgacgtaa tcgcgcaaca gcgcggacag ctcgtcgcga aacgaggcct gtccgaccca
       61 ctcacgatgt gcgcgctcga gctcgtcgag ggccgccatc agcgtttccg ggacgaaccg
      121 gccgccgtac tcgccgaagt agccgcggct atcgggtccg gtcgcgctca tccgcctgcc
      181 tcaccgcccg cacgaaggcg tgcagcttgt cggggtcctt tctgccgggg tagcgctcga
      241 cccccgagca cgcgtcgacg ccgtacggat cggcgcctga gagcgcccgc ggcacgttgg
      301 ccgccgacag cccgccggcg acgaccacac gctggcccag ccgacgggcg atcgaccagt
      361 cgaacggtcg gccgttgccg gccccggggt cgagcaagaa cgccgcgacg tcgtaccggc
      421 gcatgcgcgc gagcaacgtc tcgccgtcgt cggttcgccc cacgcggata cgcttgatga
      481 ccggtcgagt caagcgcccg cagagcgagg gcggctcgtc gccgtggagc tggatcatgt
      541 cgacgccgct ttcccgcacc gtccgcacca gtcggtcgtg gctctcgtcg acgaagacgc
      601 cgaccgtgcg tacgtccgcc ggcagacgct cgatgatccg ccgggcgcgc gcgggctcga
      661 tccggcgcgg cgagcgcgcg aagacgaagc cgatcgcatc ggccccggcg cgaacggcgt
      721 cgagcgcatc gtcgagctcc gtcacgccgc agatcttgac ccacgatcgc cgcggctcca
      781 tccggcgacc ggtccacgcc tcgatcagtt ggcgcgggtc gtcggcccgc atcagcgcct
      841 cgccgaccag taccgcatcg aagcccgcct cgcgcacgcg ccgcgcgtcc tcggccgttt
      901 cgaccccgct ctcggcgacg gcgatcagcc ccgcgggaac cgtcgcgcgc aacgagatcg
      961 tccgcgacag gtcgacgcgg aaggtatcga ggtcgcggct gttcagcccg acgatcgtcg
     1021 cgccggcgtc gaccgcgcgc gccaggtcgt gctcgtcgtg gacctcgacc agcggttcga
     1081 tcgcgagctc gcgcgtcgtc cgtatcgcgc gggccaacga ccggtcgtcc aggatgcgga
     1141 cgatcagcaa cagcgcgtcg gcgccgagag cggccgtctc gtggatctgg tacgggtcga
     1201 cgatgaactc cttgcgcagg accgggagcg tcgacgccga ccgcgcacgg atcaggtcgt
     1261 cgtcacaacc ggcgaagaac tcgcgatcgg tcaccacgga aatcgcggcg gccccgccgc
     1321 gctggtagtc gatcgcacga agcgcggggt cggcgtcgat gcggatggcg ccgctggacg
     1381 gcgagcgtcg cttgaactcg gcgatcagcg acaagccgtc ccggcccagc gctgcggcga
     1441 agctgcggat cggctcccgg cgggccgtcg ccgtcgcatc gagcgggcag gcgagtcggc
     1501 gccgcgcgac gtcggcgcgc ttggcacgga cgatccgttg cagcacgtca cgcaacgcgc
     1561 aaccccgcga agcgtcgcag cgcatgcagc ttctcgaccg cggcgcccga gtcgatcgac
     1621 tcgcgcgccc gctcgatgcc ggtctcgatc gactcggcga ctccgccgac gtagatcgcc
     1681 gcaccggcgt tgatcagcgc gacgtcgcgt gccggccccg gccgtccctc gagcacggcg
     1741 agggcgatgc gcgcgttttc gcgcggagat ccaccgcgca ggcggccggc cgcgacgcga
     1801 gcgaagccga accgccgcgg gtcgagcctg taccgatcga gtgtgccgtc ccgccactcg
     1861 cagacgtggg tcggtccgcc gagcgtgatc tcgtcgagtc cgtccgcgcc gtgcacgacg
     1921 acgcagtggg tcgaccccag gcgtgccagg accttggcga ccacctcggt cagctcgcgc
     1981 tcgaacacgc cgacgagctg gcgtcgcact ccggcgggat tggtcagcgg cccgacgatg
     2041 ttgaagatgg tgcgcaggcc gatctcgcgg cgcggttcga ccgcgtggcg catcgcccgg
     2101 tggtagtccg gcgcgaacag aaagccgaca ccgacctcgg ccaacagccg ggcggtgagc
     2161 cgggccgagg cgcgagggtc gatacccagc gcctccagcg cctcggcgct gccgcagcgg
     2221 ctcgagaccg agcggttgcc gtgcttggcg actcgacatc cggcgccggc cgcgacgaac
     2281 gcgctgaccg tcgacacgtt gaaggtcgaa cgcccgtcgc cgccggtgcc gcaggtgtcg
     2341 atcgcgtcgt cgaacggatg gcgcaggggc tcggccgcat cccgcatcgc gcgggcgaag
     2401 ccggtgatct ccgcgacggt ctcgcccttg
//
LOCUS       DASPGN010000352         8991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1232961, whole genome shotgun sequence.
ACCESSION   DASPGN010000352 DASPGN010000000
VERSION     DASPGN010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232961"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VD788_10045"
     CDS             <1..1266
                     /locus_tag="VD788_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HXV76648.1"
                     /translation="LYRNNGDGTFTDVAPAAGVADERWGVTAGWSDVDLDGDLDLYVT
                     NYVVFTFERYPIRGAPMPDGKPPCMWRGIEVYCGPRTLIPEADVLFRNDGDTDGDGVP
                     SFVDRTRDAGLLLDEAHFGLGVLFFDGDGDGDGDLYVANDSVRNLYFVNDGRGRFLES
                     SILAGVAYNEQGNEQAGMGLASGDYDRDGRFDLAVTNFSHDHDTLYHNDGNGYFTDLS
                     YPAGIGSPSFLTLGWGIAFVDLDQDGWEDLYVCHGHVYPQVDDYELGTSYRQPNAVFR
                     NSRDGTFEPLDSAGAGQHLVKSSRALAPVDLDGDGDLDLLVTSLNDSPDLLLNDGATG
                     NWLQLRLIGRRSSRDGIGARVILDSAGERQYREVRRSTGFAASTLPIAHFGLGPATTI
                     ERVEVRWPSGAVSQLTDLPANRRVEVVEP"
     gene            complement(1270..2466)
                     /locus_tag="VD788_10050"
     CDS             complement(1270..2466)
                     /locus_tag="VD788_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV76649.1"
                     /translation="MTRRRYKILHLITRLDLGGAQQNTLYCCAHHDRSRFAVRLIAGA
                     GGVLDDEARRLDDVDVRIVPWLKYAVSPASDALALLRLRAQMIRERIDLVHTHSSKAG
                     LLGRVAAHLAGVPVVVHTAHGWSFNDTQPVLVRAAYVALERFAARLTDRLITVSSRHR
                     RVGLEQGIGTPDGTIVIRSGIDVAAWATPRVERESVRRQLGIEPHHRLVGCVACLKPQ
                     KAPLDFVRAAAAAHAKDTDLRFVIAGDGELRPAVAAEIGRHGLDGVVRLLGWRRDVAD
                     LLHAMDLFLLTSRFEGLPRAVLQAAASGVPIVASDVDGTPEVIEHRTSGMLFPPGRPE
                     LAAQSVLELLRDAELRARCVREARARLTGEFDIAEMVRRLDRLYLDRLERPVQPAFEG
                     NAIHPV"
     gene            complement(2515..4380)
                     /gene="asnB"
                     /locus_tag="VD788_10055"
     CDS             complement(2515..4380)
                     /gene="asnB"
                     /locus_tag="VD788_10055"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXV76650.1"
                     /translation="MCGICGSVALDGETLSPVAVRAMLAAMVHRGPDGEGLFEERGIV
                     AGARRLAVIDLASGDQPLANEDGSVQVVFNGEIYNFAELRQSLVSRGHRFRSRCDTEV
                     LVHLWEEFGTELVHRLNGMFCFCIHDRRTGETFIARDRLGIKPLFFATVHGRLHFASE
                     ISVLLRHPEIPAEVDSSVLTELYCLQFVSGDRTVFREIRKLLPGHAIHVKDGRLSLDC
                     WYEIPPPLERDDTGRTVRQVELRELLRQAVEMCTVADVPLGTFLSGGIDSTIVTGLLA
                     RSSDHPVQTFSVGFADARAFDERRFAREAAEYYRTEHRELVVSPVDIAEHLPRLVERQ
                     CLPLMDPAAIPTYLLSRFAREHVTVVLTGEGADELFGGYKRYLYQHRFGWLARLPGAR
                     GVGRGALGGLVPPRIEQALAAMTDDDVALSHVQWASVVGFRVAASLFDEDAFTEHRRR
                     IGERYAGYFGGERRLADQLRADLSEWLPHNLLAKVDHASMAVSLEARVPFLDHRIVEW
                     ATTLPDDLRIRGGMTKAVLRDAFRDRLPPAIVERDKRGFDLPLGQWIRGPLRELTEDL
                     LTARRLAGWPGLKVGAARELLERHLSGRQEFGLPLFGLLSVMLFLERVAPVPRAS"
     gene            complement(4387..7749)
                     /locus_tag="VD788_10060"
     CDS             complement(4387..7749)
                     /locus_tag="VD788_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV76651.1"
                     /translation="MLRALIHYWRVTGAVVAGAAVATAVLSGALMVGDSVRGSLRRLT
                     LDRLGAVERALVGERFFREELASELSAALDGTLVVPLIIAQGSAIQPDSGARASRVEI
                     HGVDERFAALFPTAPTLDFARSPTQPFPSAIVNRSLADELRVAEGEDLLLGFGTSSDV
                     PRETLMGESDPDDVLASLRVTVTRIVPDAGVGRFGLAPNQIDPFNVFVALGGVQRAVE
                     QRRRVNALLVGETATGIEPGEVLERTLTFEDLGLELTLADDHATLGSRDFVLRPDVGR
                     AATEVLDALGALGQPVLTYLANRLRHGDEVVPYSSVTAVDPLDGPDWASFEWHSRRGP
                     DSGPRADGIVLNRWTADALHAAEGDTIELEYFEVGPREELILREHRFRVAGVVEPRGL
                     AGDPGLTPEYPGIQQAEDMAGWDPPFPVDLGLIGDRDEEYWDRYRALPKAFVGYDTGA
                     RLWSTRFGSITTIRFGAAPGSDVAGTAERLRRELRSRLAPAAFGFRLRPVKEEGLRAS
                     AGATDFGGLFIGFSLFLIASAAMLAGLLFGLGVERRAGEIGLLLAVGYRLRRVRGRLL
                     GEGASIAMLGALFGLGLGVAYAALMMVGLRTIWAAAVGSSRLVLFVEPSSLALGWVLS
                     VVVVLTSIWFRVERLKRIAPARLLAGSLAGGPVRHRAGLPRWIALAAASAATTLVVVG
                     LVTGRPDDPALAFGSGALLLVAGLATFAWWCRGAGRRRGVHRVAGGLTGLAARNSAWN
                     PGRSILSAALVACACFVIVVVAANRGQSSRDMRALESGSGGFPLLAESRVPLFGRLDS
                     RADLLELGFDAGVATRLADNLVVPLRSMPGDDASCLNLYRPERPRILGVPPQLVERAG
                     FTFRQALPLADSAASPWDLLEAPSADGTIPAIGDHNSTTWILRLGLGDGIELTDELGR
                     TVTLRLVATLDTSVFQSELLISEADFLEHFPGRSGYGYFLIDTPSRALDETAALYERT
                     LAPFGFDVSTSAEKLESFKVVEHTYLATFQLLGGLGLLLGTVGLAVILLRNVIERRGE
                     LATLRAFGFRRASLARMILIENLFLLVVGVSIGTVAALVAVGPRLLRLDVPWLSIATT
                     LGIVVAVGMLASVAAVRGALRVPLLPALKAER"
     gene            complement(7734..8402)
                     /locus_tag="VD788_10065"
     CDS             complement(7734..8402)
                     /locus_tag="VD788_10065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV76652.1"
                     /translation="MLSLSGVRKSFDLGPSPVEVLSGVSFTIGAGEAMVVTGPSGSGK
                     STLLHLIGTLEQPSSGRIEIDGRDPFAATEPELARFRNANVGFVFQDHHLLPQYSVLE
                     NVLVPTLAFPRKDRAAGERARELLDRVGLAHRLDHRPAELSGGERQRVAVARALINRP
                     RLLLCDEPTGSLDPRSAGSVAELLFELHGEESNVMIVVTHSLELASRFARRCELREGR
                     CCER"
     gene            complement(8408..8833)
                     /locus_tag="VD788_10070"
     CDS             complement(8408..8833)
                     /locus_tag="VD788_10070"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXV76653.1"
                     /translation="MHELHTTRRVEFADTDMGGIVHFSRYLVFMETAEHEFLRAAGTD
                     VDLRLPDGRRIGWPRVAVSCEYKSPARYGDLLDIHVRVLRKGRSSLSYGIRFSCGGRE
                     VAEGRVTSVCCELAPGEEVRSIPIPDSIARRIDQPPDGR"
BASE COUNT         1512 a         3178 c         3061 g         1240 t
ORIGIN      
        1 ctctatcgca acaacggtga cgggaccttc accgacgtcg cgccggccgc cggggtggcc
       61 gacgagcggt ggggcgtgac ggcgggatgg agcgacgtcg atctcgacgg ggatctcgac
      121 ctgtacgtga cgaactacgt cgttttcacc ttcgagcgct acccgatccg tggcgccccg
      181 atgcccgacg gcaaaccgcc gtgcatgtgg cgcggaatcg aggtctactg cggcccgcgc
      241 acgctgatac ccgaagccga cgtgctgttc cgcaacgacg gcgacacgga cggtgacggc
      301 gtcccgagct tcgtcgatcg tacgcgtgac gccggcctgt tgctcgacga ggcccatttc
      361 ggtctgggcg tgctgttctt cgacggcgac ggcgacggcg acggcgacct gtacgtggcg
      421 aacgattccg tgcggaactt gtatttcgtc aacgacggcc gaggccggtt cctggaatcc
      481 tcgatcctcg ccggtgtggc ctacaacgag caaggaaacg aacaggccgg catgggcctg
      541 gcgagcggcg actacgatcg tgacggccgc ttcgacctgg ccgtcaccaa cttctcgcac
      601 gaccacgaca cgctctatca caacgacggc aacggctact tcaccgacct atcctatccc
      661 gcgggaatcg gcagcccgtc gttcctcacg ctcggctggg gcatcgcgtt cgtcgacttg
      721 gatcaggacg gttgggagga tctgtacgtc tgtcacggtc acgtctaccc gcaggtcgac
      781 gattacgagc tcggcacctc gtatcgacag cccaacgcgg tgttccgtaa ctcgcgtgac
      841 ggcacgttcg aaccgctcga ctcggccggc gcgggtcagc acctcgtcaa gtcctcgcgg
      901 gcgctggctc cggtcgacct cgacggcgac ggcgatctcg atctgctcgt cacctcgctc
      961 aacgactcgc ccgacctgtt gctcaacgac ggcgcgacgg gcaactggct gcagctgcgg
     1021 ttgatcggtc gccgtagctc gcgcgacggg atcggcgcac gggtgatact cgacagcgcc
     1081 ggcgagcggc agtaccgcga ggttcgccgc tcgaccggct tcgccgcttc gacgctgccg
     1141 atcgcgcact tcggtctcgg cccggcgacg acgatcgagc gcgtcgaggt tcgctggcca
     1201 tcgggggcgg tcagccagct gaccgatctg ccggcgaacc ggcgggtcga ggtagtggaa
     1261 ccgtagcgct cagaccggat ggatcgcgtt gccctcgaac gcgggctgga ccggccgctc
     1321 cagacggtcg aggtagagac ggtccagtcg tcggaccatc tcggcgatgt cgaactcgcc
     1381 cgtcagccgg gcgcgggcct cgcggacgca gcgcgcacgc aactcggcgt cgcggagcag
     1441 ctccaggacc gactgcgcgg ccagctccgg tcgtccgggc gggaacagca tgccgctcgt
     1501 gcggtgctcg atcacctcgg gcgtgccgtc gacgtcggac gccacgatcg gcacgccgct
     1561 ggccgccgcc tgcagcaccg cccgcggcaa gccttcgaag cgcgaggtga gcaggaacag
     1621 gtccatcgcg tgcagcaagt cggcgacgtc ccgccgccag ccgagcagcc ggacaacgcc
     1681 gtccaacccg tggcggccga tctccgcggc gaccgccggg cgcaactcgc cgtcgccggc
     1741 gatgacgaat cgcaggtcgg tgtccttcgc atgggccgcg gccgcggcgc ggacgaaatc
     1801 gagcggcgcc ttctgcggct tgaggcaggc cacgcaaccg acgagccggt ggtgcggctc
     1861 gataccgagc tgccggcgaa ccgattcgcg ctcgacccgc ggggtagccc acgcggcgac
     1921 gtcgatcccg ctgcggatga cgatcgtgcc gtccggcgtg ccgatgcctt gctcgagccc
     1981 gacccgacgg tggcgggacg agacggtgat caatcggtcg gtcagtctcg ctgcgaaccg
     2041 ctccagcgcg acataggccg cgcgaacgag caccggctgc gtgtcgttga agctccaccc
     2101 gtgggccgtg tggacgacga ccggcacgcc ggcgaggtgc gccgcgaccc ggccgagcag
     2161 accggccttg gaagagtgcg tgtgcaccag gtcgatccgt tcgcggatca tctgcgcccg
     2221 cagtctcagc agcgcaagtg cgtcactcgc cggcgagacg gcgtatttca gccacggcac
     2281 gatccgtacg tcgacgtcgt ccagtcgccg cgcctcgtcg tcgagcacgc cacccgcgcc
     2341 ggcgatcagt cggaccgcga atcgggagcg gtcgtggtgt gcgcagcaat agagcgtgtt
     2401 ctgctgggcc ccaccgagat cgagccgggt gatgagatgc aggatcttgt agcgtcgtcg
     2461 ggtcatctcg gcagaatgta gccctggttc gcggcgaggt ccataagtcg cgagtcagct
     2521 cgcacggggc acgggcgcga cgcgctcgag aaacaacatg acggagagca gaccgaacag
     2581 cggcaagccg aattcctggc ggcccgacag gtgccgctcg agcagctccc gcgcggcccc
     2641 gaccttcagc cccggccaac ccgcgagtcg tcgcgccgtc aacaggtcct cggtcagctc
     2701 ccgcagcgga ccgcgaatcc actgtccgag cggcagatcg aagccgcgtt tgtcgcgctc
     2761 gacgatcgcg ggtggcaggc gatcgcgaaa cgcgtcgcgc agaacggcct tggtcatccc
     2821 gccgcgaatc cgcagatcgt cggggagcgt cgtcgcccac tcgacgatcc ggtggtcgag
     2881 aaacggcacg cgcgcttcga gcgaaacggc catgctcgcg tgatcgacct tggccagcag
     2941 gttgtgcggc agccattccg acaggtcggc gcgcagttga tcggccagac gccgctcgcc
     3001 gccgaagtag ccggcgtagc gctcgccgat tcggcggcga tgctcggtga aggcatcctc
     3061 gtcgaacagg ctggccgcga cgcgaaagcc gacgaccgac gcccattgca cgtgactcag
     3121 cgccacgtcg tcgtcggtca tcgcggccag cgcctgctcg atccgtggtg gcacgagccc
     3181 acccagcgcg ccgcgaccga cgccacgcgc acccggcagg cgagcgagcc agccgaagcg
     3241 gtgctggtac aggtagcgct tgtagccgcc gaacagctcg tcggcgccct cgccggtcag
     3301 caccaccgtg acgtgctccc gggcgaatcg tgacaacagg taggtcggga tcgccgccgg
     3361 gtccatcagc ggcagacact ggcgttcgac caagcgcggg aggtgctcgg cgatgtcgac
     3421 cggtgagacc accagctcgc ggtgctcggt acggtagtac tcggcggcct ctcgtgcgaa
     3481 gcggcgctcg tcgaaggcgc gggcgtccgc gaaaccgacg gaaaaggtct gcaccgggtg
     3541 gtccgagctt ctcgccagca atccggtgac gatcgtcgag tcgatcccac ccgacaggaa
     3601 cgtgccgagc ggcacgtcgg ccacggtgca catctcgacc gcttgccgga gcaactctcg
     3661 cagctcgacc tgccgtaccg tccgcccggt atcgtcgcgc tccaagggtg gaggaatctc
     3721 gtaccagcag tcgagcgaca gccgtccgtc cttgacgtgg atcgcgtgcc ccggcagcag
     3781 cttgcggatc tcgcggaaca cggtccgatc gccgctgacg aactgcaggc agtacaactc
     3841 ggtcagcacc gacgaatcga cctccgccgg aatctcgggg tgacgcagca gcaccgagat
     3901 ctccgaagcg aagtgcagcc ggccgtggac cgtggcgaag aacagcggtt tgatcccgag
     3961 ccggtcgcgc gcgatgaacg tctcgccggt gcgtcggtcg tggatacaaa agcagaacat
     4021 gccattcaag cggtggacca gttcggtgcc gaactcctcc cacaggtgga caagcacctc
     4081 ggtgtcgcag cggctgcgaa atcggtgccc ccggctgacg agcgactggc gaagctcggc
     4141 gaagttgtag atctcgccgt tgaacacgac ctggaccgac ccgtcctcgt tggccaacgg
     4201 ttgatcgcct gaggcgaggt cgatgaccgc cagccgccgc gcaccggcga cgatcccgcg
     4261 ctcctcgaac agcccctccc cgtcggggcc gcggtggacc atcgccgcga gcatcgcgcg
     4321 caccgccacc ggagagagag tctcaccgtc cagcgccacg cttccgcaga tcccgcacac
     4381 gggcgttcag cgttccgctt tgagcgccgg cagcagcggg acccgcaacg cgccgcgtac
     4441 tgcggcgacg ctggccagca taccgaccgc gaccacgatg ccgagcgtgg tcgcgatcga
     4501 aagccagggc acgtcgaggc ggagcagccg cggcccgacg gcgaccagcg cggcgacggt
     4561 gccgatcgag accccgacga ccaacaggaa caggttctcg atcaggatca ttcgtgccag
     4621 tgacgcgcgc cgaaagccga atgcgcgcag cgtcgccagc tctccgcgcc gctcgatcac
     4681 gttgcgcagc aggatcaccg ccaaaccgac ggtcccgagc aacaggccga gtccgccgag
     4741 cagctggaag gtcgccaggt aggtgtgttc gacgaccttg aagctctcga gcttctcggc
     4801 gctggtcgac acgtcgaagc cgaaaggcgc gagcgtgcgc tcgtagagcg ccgccgtctc
     4861 gtcgagcgcc cgcgacggcg tatcgatcag gaagtacccg tagccggagc ggccggggaa
     4921 gtgctcgagg aaatccgcct cggagatgag cagctcgctc tgaaacacgc tggtgtcgag
     4981 cgtggccacc agacgcaggg tgaccgtccg tccgagctcg tcggtcagct cgatcccgtc
     5041 acccaacccc agacggagaa tccacgtcgt cgagttgtgg tccccgatgg cggggatcgt
     5101 cccgtccgca gacggtgcct cgagcagatc ccacggactc gcggcggaat cagctagcgg
     5161 cagtgcctgc cggaaggtga acccggcccg ctcgacgagc tgggggggga cgccgaggat
     5221 ccgcggccgc tcggggcggt agaggttcag gcagctggcg tcgtcgccgg gcatgctgcg
     5281 cagcggcacg acgaggttgt ccgcaagacg tgtggccacc ccggcgtcga acccgagctc
     5341 gagcaggtcc gcacgagaat cgagtctccc gaacagcggg acgcgggatt cggccaacaa
     5401 cgggaaaccg ccggatcccg actcgagtgc ccgcatgtcg cggctcgact gaccgcgatt
     5461 ggcggcgacg acgacgatca cgaagcaggc acaggcgacg agcgccgcgc tcagtatgct
     5521 gcgtccgggg ttccatgcgc tgttccgtgc cgccagtccc gtcaacccgc ccgcgacgcg
     5581 gtgcacgccg cgtcgtcgtc cggcgccgcg gcaccaccag gcgaacgtcg ccaggccggc
     5641 gaccaagagc agcgccccgc tgccgaaggc cagcgcagga tcgtccggtc gcccggtgac
     5701 caggccgacg acgaccaacg tcgtggccgc cgaggcggcc gccagagcga tccagcgcgg
     5761 caggccggcc cggtggcgga ccgggcctcc agccagcgac ccggccaaca gccttgccgg
     5821 agcgatccgc ttcagccgct cgacgcgaaa ccagatcgac gtcagcacga cgacgaccga
     5881 gagcacccag ccaagcgcga ggctcgacgg ctcgacgaac agcacgagcc gggacgaccc
     5941 gaccgcggcg gcccagatcg tgcgcaaccc gaccatcatc agcgcggcat aggccacccc
     6001 cagcccgagg ccgaacaacg ccccgagcat cgcgatcgac gcaccctcgc cgagcaggcg
     6061 cccgcgaacc cggcgcagcc tgtagccgac cgcgagcagc aggccgatct cgcccgctcg
     6121 tcgctcgacg ccgaggccga acaacaggcc ggcgagcatc gccgcggagg caatcagaaa
     6181 caggctgaag ccgatgaaca acccgccgaa atcggtcgcg cccgccgacg cgcgcagccc
     6241 ctcttccttg accggccgca gccggaagcc gaacgcggcc ggcgcgagcc gcgatcgcag
     6301 ctcccggcga aggcgctcgg ccgtgccggc gacgtcgctc cccggcgcgg cgccgaagcg
     6361 gatcgtcgtg atcgagccaa agcgggtact ccacagccgg gcgccggtgt cgtagccgac
     6421 gaacgccttg ggcagcgcgc ggtagcgatc ccagtactcc tcgtcgcggt cgccgatcag
     6481 cccgagatcg accgggaacg gcgggtccca accggccatg tcttcggcct gctggatgcc
     6541 ggggtactcc ggtgtcaggc ccgggtcgcc ggcgagcccc cgcggctcga cgacgccggc
     6601 cacgcgaaac cggtgctcgc gtaggatcag ctcttcgcgc ggcccgacct cgaagtattc
     6661 cagctcgatc gtgtccccct cggcggcgtg cagcgcgtct gccgtccagc gattgagcac
     6721 gatcccgtcc gcgcgcgggc cgctgtccgg cccgcgccga gagtgccact cgaacgacgc
     6781 ccagtccggg ccgtcgagcg ggtcgacggc ggtcaccgac gagtagggca cgacctcgtc
     6841 gccgtggcgc agacgattgg cgagataggt cagcacgggt tgcccgagag cgccgagcgc
     6901 gtcgagcacc tcggtcgccg cccgaccgac gtccggtcgc aggacgaaat cgcggctccc
     6961 cagcgtggcg tgatcgtccg cgagcgtcag ctcgagcccg aggtcctcga aggtcagcgt
     7021 ccgttccagc acttcgccgg gctcgatacc cgtggccgtt tcgccgacca agagcgcgtt
     7081 gactcgacgg cgttgctcga ccgcgcgttg cacgccgccc agcgccacga acacgttgaa
     7141 cgggtcgatc tggttcggtg ccagaccgaa gcgcccgacc cccgcgtcgg ggacgatccg
     7201 cgtgacggtc acccgcagcg aggccaacac gtcgtccgga tcggactcgc ccatcagcgt
     7261 ttcgcgcgga acgtcgctcg acgtgccgaa gccgagcagc aggtcctctc cctcggccac
     7321 acggagctcg tcggccagcg agcggttgac gatcgccgag gggaagggct gcgtcggcga
     7381 gcgcgcgaag tccaaggtcg gtgcggtggg gaacagagcg gcgaagcgct cgtcgacacc
     7441 gtggatctcg acacgcgatg cacgggcgcc ggagtcgggc tggatcgcgc tgccctgtgc
     7501 gatgatcagc ggcacgacga gcgtaccgtc cagtgccgcg gacagctcgc tcgccagttc
     7561 ttcgcgaaag aagcgctcac cgaccagcgc ccgctcgacg gcgccgagcc ggtcgagtgt
     7621 gaggcggcgg aggctgcccc gcaccgagtc accgaccatc agcgcgcccg acagcaccgc
     7681 ggtggcgacc gcggccccgg cgacgaccgc gcccgtcacg cgccaatagt ggatcagcgc
     7741 tcgcagcatc ggccctcccg cagctcgcac cgtcgggcga agcgcgaggc cagctccagg
     7801 ctgtgggtca cgacgatcat cacgttcgac tcctcgccgt ggagctcgaa cagcagttcg
     7861 gcgaccgatc cggccgagcg cggatcgaga ctccccgtcg gttcgtcgca cagcagcagc
     7921 cgcgggcggt tgatcaatgc ccgggccacg gcgacccgct gtcgttcccc gcccgacagc
     7981 tcggcggggc gatgatcgag ccggtgggcc agtccgaccc ggtccaacag ctcgcgtgcg
     8041 cgctcgcccg ccgcgcggtc ttttcgcggg aaggccagcg tcgggaccag cacgttctcc
     8101 agcaccgagt actggggcag cagatgatgg tcctggaaga cgaagccgac gttggcgttg
     8161 cggaaccgag ccagctccgg ttcggtggcg gcgaacgggt cgcgaccgtc gatctcgata
     8221 cgacccgagc tcggctgctc gagcgtgccg atcaggtgca gcagggtact cttgccggag
     8281 cccgagggtc cggtgacgac catcgcttcg ccggcgccga tggtgaagct gacgccggat
     8341 agcacctcga ccggggacgg cccgaggtcg aagctcttgc gaacgccgct caggctgagc
     8401 atgcggctca ccttccgtcc ggcggctggt cgatcctgcg tgcgatggaa tccgggatgg
     8461 ggatcgaccg cacttcctcg cccggcgcca gctcgcagca gaccgaggtg acgcgaccct
     8521 cggctacctc ccgccccccg caactgaacc gaattccgta gctcagcgac gatcggccct
     8581 tgcgcagaac gcgaacgtgg atgtcgagca gatcgccgta gcgcgccggg ctcttgtact
     8641 cgcaactgac ggcgaccctc ggccaaccga tcctccgccc gtccggaagc cgcagatcga
     8701 cgtcggtccc cgccgcccgc agaaactcgt gctcggcggt ctccataaag acgaggtatc
     8761 gggaaaaatg gacgatcccg cccatgtcgg tatcggcgaa ctcgacgcgc cgggtggtgt
     8821 gcagttcgtg catcagcggg tcctattctc cacgtattcg cggtacacgt tcaagacgtc
     8881 gtcggccgcc tgctcgtcgc ggaactcgcg gtggaccgcc tcgtagccgg cgagaccgag
     8941 gcgccggcgc agcgcggtat cggccatcag tcgctcgagg gcgtcggcca g
//
LOCUS       DASPGN010000353         2148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1466625, whole genome shotgun sequence.
ACCESSION   DASPGN010000353 DASPGN010000000
VERSION     DASPGN010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1466625"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VD788_10075"
     CDS             complement(<1..771)
                     /locus_tag="VD788_10075"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HXV76654.1"
                     /translation="MATPIDLRSDTVTRPGPDMRRAMAAAVVGDDVYGEDPTVRALEE
                     RAAVIVGHPSALFVPTGTMGNQIAIQLHTQPGCDVLVEQDSHVYRYELGAMAAWSGAL
                     ARVIVGDGGRITPEQIDERIAPDVYYMSPTRLLVLENTHNHAGGTVLDLEQSSRLVAR
                     ARHHRLALHLDGARLFNAAAALGVDAERLAAGFDTVMFSLSKGLGAPVGSVLCGTTES
                     MRRARVIRKRLGGGMRQAGILAAAGLFALEHNLDRLVED"
     gene            complement(786..1556)
                     /locus_tag="VD788_10080"
     CDS             complement(786..1556)
                     /locus_tag="VD788_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76655.1"
                     /translation="MRRAARLLRIGCACLALGTAAPAAADSPERIFERGNSAYDAGAY
                     AEAADAYRTLLKYQILDPRLEYNLGNAEFRLGNLGRAILHYERARRLDPSDRDIQANL
                     AFARSLRLDRVPPAERPAAVAWVVGIQDRLGPDPQAWTLVALLWLLGAVVAWGLAERG
                     RWSGGLGWCSATLALLIGLTSMSWYATHTRVEGQTTAVVVDEATEVLAGPGQNNATLA
                     TIHEGLDVEVWGERNEWIQVRLPNGVSGWVAKRAIEIV"
     gene            complement(1556..>2148)
                     /locus_tag="VD788_10085"
     CDS             complement(1556..>2148)
                     /locus_tag="VD788_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76656.1"
                     /translation="EPIRLAVERGEGDGDPTAIRSEIQMQRRDLAYIKPLRGELTQRH
                     APAHRRTVFRLALVAPVLLTPLVIMLGRRQARLRRDHGLSRARRAGTKARRKLQSIDK
                     RIDQLEVGAFHEELARTLVEYVADRFNRSAAGMTYELAEELLASRSVPDALRRRFRSC
                     LETCDFARFVPSAGKSERRIELLTEAREVVDGLERG"
BASE COUNT          314 a          784 c          739 g          311 t
ORIGIN      
        1 atcctcgacc agccggtcga gattgtgctc gagcgcgaac agcccggcgg cggcgaggat
       61 gcccgcctgg cgcatgccgc cgccgagtcg cttgcggatc acgcgcgccc gccgcatcga
      121 ttcggtcgta ccgcacagca ccgagcccac cggcgcgccc aaccccttcg acagcgagaa
      181 catcaccgtg tcgaatcccg ccgccagccg ctcggcgtcg acgccgagcg cggccgccgc
      241 gttgaacaga cgggcgccgt cgagatgcag cgcgagacgg tgatggcgcg cccgagcgac
      301 cagtcgcgag ctctgctcga ggtcgagcac ggtgccgccg gcgtggttgt gggtgttctc
      361 cagcaccaac agccgcgtcg gcgacatgta gtagacgtcg ggcgcgatcc gctcgtcgat
      421 ctgttcgggc gtgatcctgc cgccgtcgcc gacgatcacc cgtgcgagcg cgccgctcca
      481 ggccgccatc gcgccgagct cgtagcggta gacgtgcgag tcctgctcga cgaggacgtc
      541 gcaccccggt tgcgtgtgca gctgaatcgc gatctgattg cccatcgtgc ccgtcggcac
      601 gaacagcgcc gatggatgcc cgacgatcac cgccgcgcgt tcttcgagcg cgcgaaccgt
      661 cgggtcctcc ccgtagacgt cgtccccgac gacggcggcg gccatcgccc ggcgcatgtc
      721 cgggcccggt cgcgtcaccg tgtcgcttct cagatcgatc ggcgtcgcca tcgtgctcgc
      781 tccattcaga cgatctcgat cgcgcgcttg gcgacccaac cgctgacgcc gttgggcagg
      841 cgcacctgga tccactcgtt gcgctcgccc cacacctcga cgtcgagccc ctcatggatc
      901 gtggccagcg tcgcgttgtt ttgccccggc ccggcgagga cctccgtcgc ctcgtcgacg
      961 acgaccgccg tcgtctgacc ttcgactcgc gtatgggtcg cgtaccacga catcgaggtc
     1021 aacccgatca gcagcgcgag cgtcgcgctg caccagccca gtcccccact ccagcgcccg
     1081 cgctcggcga gaccccaggc gacgacggcg ccgagcaacc acagcagcgc gacgagcgtc
     1141 caggcctgcg gatcgggccc caggcggtcc tggatcccga cgacccaggc gaccgccgcc
     1201 ggccgctcgg cggggggcac gcggtcgagc cgcagcgagc gggcgaaggc caggttggcc
     1261 tggatgtcgc gatccgacgg gtcgagacgc cgggcccgct cgtagtgcag gatggcgcgc
     1321 ccgagatttc cgagtcggaa ctcggcgttg cccaggttgt actcgagacg cggatcgagg
     1381 atctggtact tgagcagcgt acggtaggcg tcggcggcct cggcataggc tccggcatcg
     1441 taggcgctgt tgccgcgctc gaaaatccgc tccggcgagt cggcggcggc gggagccgcg
     1501 gtgccgagcg ccaggcaagc gcagccgatc cgtaacagcc gcgcggcccg gcgcatcagc
     1561 cgcgctccaa gccgtcgacc acctcgcgcg cctcggtcaa gagctcgatg cgtcgttcgg
     1621 acttgccggc ggacggtacg aaccgtgcaa aatcgcaggt ctcgagacac gaacggaacc
     1681 ggcggcgcag cgcgtcgggg accgaccgcg acgccaacag ctcctcggcc aactcgtagg
     1741 tcatgccggc cgcgctgcgg ttgaaccggt cggcgacgta ctcgacgagc gtgcgggcca
     1801 gctcctcgtg gaacgcgccc acttcgagct ggtcgatccg cttgtcgatc gactgcagct
     1861 tgcgccgcgc cttggtgccc gcgcgacgcg cccgcgacag cccgtggtcg cggcgcagcc
     1921 gcgcttggcg ccggccgagc atgatcacga gcggggtgag caggaccggt gcgacgagcg
     1981 cgagtcgaaa gacggttcgt cggtgcgccg gcgcgtggcg ctgggtcaac tctccgcgca
     2041 gcggcttgat gtaggccaag tcgcgacgct gcatctggat ctcgctgcgg atcgcggtcg
     2101 ggtcgccgtc gccttcaccg cgctcgaccg ccagccggat cggctccg
//
LOCUS       DASPGN010000354         6548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_179556, whole genome shotgun sequence.
ACCESSION   DASPGN010000354 DASPGN010000000
VERSION     DASPGN010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6548
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179556"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2294)
                     /locus_tag="VD788_10090"
     CDS             complement(<1..2294)
                     /locus_tag="VD788_10090"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV76657.1"
                     /translation="MSPFSWPVRRPVATSMAFAGIFFLGLIAWLRIPVELMPAVSGDD
                     LFVNFNRPGSEPEVVEREILLPLQARVSELPHVDETWAEIRDSGGTFTVRFEPGSDLK
                     VRELELRRLATDLVRTQPRGTLIDVSALDLTAFSRFVMFVQVTGMEDRNSLLDFVEQR
                     VVPRLLAVAGVSRVMAGGGAPRELTVRIDPDRCAAMGVLPEQVTAALARTVSRERFLG
                     GIEDDAGRTVVVLDGRPRGVIALADTRVAADRAVLLRHVADVELGTGREERLFRVNGK
                     PTVAIVVFQDETANLIRLGRDLRARLDGLREEFRPYGIDFVVNFDSSELVESQLTRLE
                     KLAVSGFLIALAVLFLFLRQWRAVAVVAIAVPASLLSALALLFLAGQTINLITLFGLA
                     VGIGMLVDNSIVVYEAVQRRLEHGGTPDQSAEAGVRRTVRAILAASVTNAVVFAPIAF
                     ADFEDAVVRALLEVMALAIILPVLASVLVAVGLVPLLARRLAAPAALASIERARRRRE
                     RQAGLVAPDLARGLFGGLLAVVLRRPAGWVASIGFAVIVTFVVAVTWFASGVSGRQPP
                     EAETVRFSVTIEDRGPLESAAASFARLEGAVAELEGVELVESMVEEEGGSLTVHLLPK
                     GERPEQTNANRVRAVVVETARELDGVSIDSQQAGLGAGGGSGPGGSGGLAALLGQGPA
                     EVVLSGPDAAELNALAREVAESLESIAEVATATTDVRQGQSEFRVAPDEIALAAFGLT
                     ADQVLPALSVVRREGVEMRTGFTL"
     gene            complement(2291..5368)
                     /locus_tag="VD788_10095"
     CDS             complement(2291..5368)
                     /locus_tag="VD788_10095"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV76658.1"
                     /translation="MQLIARLATRRPVAVTVVATAVVILGWTAWRSLPLDLLPDLESP
                     TVVVSIRSGDRPPAEMERIYGEQVEQRLFAVTGIREINQVARTGRIIATVVFEWETEM
                     DFALVDVEKAVGPIRSDPDVDEILVRHFDPRQAAVLTIGLLTDEGGPDLAELQRIARR
                     QVATTLERLPGVAEVRVTGGREKQVQVRVDRYKLEAYGVALPELEERLRAANVDINAG
                     TLEEGGKVFLVRGLARYREASDVGEVVVRFGQDDDGRRVPVRVSDLGRVRVEDAEITH
                     LVRVDGREGVGLSIYKEAGANTVEVSRTVREALGSLRGDLPGIDVHVVADEAALVEDA
                     IADVQRGALIGIGLAMLVLALFLRAAGPTLIVATTVPVSLLGTLFLMRFGDQTLNIMT
                     LGGLALGAGMLVDNAIVVVESIYRRLASGESGEDAVARGTADVGGAILASTLTTCAVF
                     LPIVFVRGLAGRLVTGLSFTVVVSLVVSLVAAVLLIPALSRWLLPQGTARPVDPGVER
                     IERIVHRLLGRPLVVVGVALGLTAGALFLLRGLGSELLPPSDPRQFSVRLVGYPGQRV
                     ESTARTVEAVEQILRQASGDDLVAMLSEVGRLPEDDRFIRSEQTEENTARVVVRLKAG
                     GATAGQVVASAAPAVAELAGIEASWEVGSSALSQALGTSGPPIVVEVSGQALSDLRLA
                     ADRLSEAMAARPELWNVRSSFEGGPPELRVVLDRTLADGLGVDLTLVTATLEASLDGL
                     RSTVLTTGDEERDVVLMLPRIRRDELLELPLTTSRGVRVSVGDVARVEPAVGAREIFR
                     RDQRRVARVTARIADGSDFPRAIAAAEGAIEAAAVPPGLDVRLAGEEEQRARTFDELR
                     FAAALAVMLVFMVLAGTFESLIHPLTVVASIPLALVGVAVVLWPIGRPLGVMELLGAI
                     VLAGVAVNDAILLVDAARRLLATGIPRREALARAAGIRLRPILMTSATTVLAMLPLAI
                     GSGEAARLRSPLALTIIGGMIASTVASLSVTPALYLILDRLRPTARRADTGPR"
     gene            complement(5375..6454)
                     /locus_tag="VD788_10100"
     CDS             complement(5375..6454)
                     /locus_tag="VD788_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV76659.1"
                     /translation="MTCRRRLAALAVGSLLLSAGCGAQGNDRTVEFRVPVFVEPVRTG
                     TVEDRIVATGTLRSLEIVSLRADTGGALAIASDPAGRRLAEGSRVDAGQVVAVITGEE
                     VRLAAGTDATRQRYEAARRDYESKKELFDEGLLSEELFRQVETALAESKIEWERSLLT
                     EARSSLVTPIGGVILRLARDENGTPLADGQLVTQGFVVAQIAPATSLIADVDLVGRDV
                     ARVVGGMPARIRHHAWEDQAFDGRVLRLAPALDPVTRTLRAEVAVQNPEGRLRPGMFV
                     EVTLIAERREDVAVVPREAVTERGGRKVVFVLNGQKVNQRDVGVGLGDDEVIEIRQGV
                     EPGERIVVRGLETLTDGTSVRPTGA"
BASE COUNT         1032 a         2472 c         2235 g          809 t
ORIGIN      
        1 tggagagtga agccggtccg catctcgacg ccctcgcgcc gtaccacgct caacgccggc
       61 agcacctggt ccgccgtcag gccgaacgcc gcgagcgcga tctcgtcggg tgccacgcgg
      121 aactcgctct gcccctgtcg cacgtcggtc gtcgcggtgg cgacctccgc gatcgactcg
      181 agcgattcgg cgacctcgcg cgccagcgcg ttcagctccg cggcgtcggg ccccgacagc
      241 acgacctccg ccggtccctg tccgagcagc gcggccagcc cgccggaccc gcccggtccg
      301 ctgccgccgc cggcgccgag ccccgcctgc tggctgtcga tgctgacccc gtcgagctct
      361 cgcgccgttt cgacgacgac cgcgcgcacg cggttggcgt tggtttgctc gggtcgctct
      421 cccttcggca gcaggtgcac cgtcaacgac ccgccttcct cctcgaccat gctttccacc
      481 aactcgaccc cttcgagctc ggctaccgcg ccctccagtc gggcgaacga cgcggcggcc
      541 gactcgagcg ggccgcggtc ctcgatcgtg acgctgaagc gaaccgtctc ggcctccggc
      601 ggctggcggc cgctgacacc gctcgcgaac caggtcaccg cgacgacgaa ggtcacgatc
      661 acggcaaagc cgatcgacgc gacccagccc gccggccgcc gcagcacgac ggccaacaga
      721 ccgccgaaca gcccgcgcgc gagatccggc gcgacgagac cggcctgccg ctcgcgccgg
      781 cgacgcgccc gttcgatcga ggccagcgcc gccggggcgg ccagccggcg tgccaacagc
      841 gggaccaggc cgacggcgac cagcaccgaa gccagcaccg gcaggatgat cgcgagcgcc
      901 atcacctcga gcagcgcgcg caccacggca tcctcgaaat cggcaaaagc gatcggggcg
      961 aagacgacgg cgttggtcac gctggccgcg aggatcgcgc ggaccgtgcg gcgcacgccg
     1021 gcctccgcgg attggtcggg cgtgccgccg tgctccagcc tgcgctgcac cgcctcgtag
     1081 acgacgatgc tgttgtcgac gagcatgccg atgccgacgg cgaggccgaa cagcgtgatc
     1141 aggttgatcg tctggccggc gaggaacagc aacgccagcg ccgacagcag gctcgccggc
     1201 accgcgatcg cgacgacggc gaccgcccgc cactgccgca ggaacaggaa cagcacggcg
     1261 agcgcgatca ggaacccgga aacggccagc ttctccaggc gggtcagctg cgattcgacg
     1321 agctcggacg aatcgaagtt gacgacgaag tcgatcccgt acggccgaaa ctcctcgcgc
     1381 agcccgtcga gccgcgcgcg cagatcgcga ccgaggcgga tcagattggc cgtctcgtcc
     1441 tggaacacca cgatcgccac cgtcggcttg ccgttgacgc ggaacagccg ctcctcccgt
     1501 ccggtgccga gttcgacgtc ggccacgtgc cgcaacaaca cggcccggtc ggcggcgacc
     1561 cgcgtgtcgg ccagcgcgat cacgcctcgc ggccggccgt cgagcacgac gaccgtgcgg
     1621 ccggcatcgt cctcgatccc gccgaggaag cgctcgcggc tgacggtgcg ggccagcgcc
     1681 gcggtcacct gttcgggcag cacgcccatc gcggcgcagc ggtcgggatc gatcctgacg
     1741 gtcagctcgc gcggtgctcc cccgccggcc atcacccgcg agacgccggc gacggccaac
     1801 agccgcggga cgacccgctg ctcgacgaag tcgagcaggc tgttgcgatc ctccattccg
     1861 gtgacctgga cgaacatgac gaagcggctg aacgcggtca ggtccagcgc gctgacgtcg
     1921 atcaacgtgc cgcgcggctg cgtgcggacg agatcggtgg cgagacgccg cagctcgagc
     1981 tcgcgaacct tgaggtcgct gcccggctcg aagcgaacgg tgaacgtgcc gcccgagtcg
     2041 cgaatctcgg cccacgtctc gtcgacgtgc ggcagctccg acacgcgggc ctgcagcggc
     2101 agcaggatct cgcgctcgac gacctcgggc tcgctacccg gccggttgaa gttgacgaac
     2161 agatcgtcgc cgctgaccgc cggcatcaac tcgaccggaa tgcggagcca cgcgatcaga
     2221 cccaggaaga agatcccggc gaaggccatc gacgtcgcca ccgggcgccg caccggccac
     2281 gagaacgggc tcatcgcggt cccgtgtcgg cgcgacgcgc cgtgggccgc agccggtcga
     2341 ggatcaggta caacgccggc gtgacggaca gcgaggcgac cgtcgacgcg atcatcccgc
     2401 cgatgatcgt caacgccagc gggctgcgca ggcgggccgc ctcgccgctg ccgatcgcca
     2461 gcggcagcat cgccagcacg gtcgtcgcgc tggtcatcag gatcgggcgc aggcggatcc
     2521 cggcggcgcg ggccaatgcc tcgcgtcgtg gaatgcccgt cgccagcagg cgacgggcgg
     2581 cgtcgacgag caggatcgcg tcgttgaccg cgacgccggc gagcacgatc gctccgagca
     2641 gctccatcac gccgagcggg cgcccgatcg gccaaagcac gaccgcgacg ccgaccagcg
     2701 ccagcgggat cgacgcgacg acggtcagtg gatggatcaa cgactcgaac gtgccggcga
     2761 gcaccatgaa caccagcatc accgcgagcg cggcggcgaa gcgcagctcg tcgaaggtgc
     2821 gcgcgcgctg ctcctcttcg ccggccagcc gcacgtccag cccgggcggg acggcggcag
     2881 cttcgatcgc accctcggcc gcggcgatcg cgcgtgggaa atcgctgccg tcggcgattc
     2941 gcgcggtgac gcgggccacc cgccgctggt cgcgacggaa gatctcgcgg gcgccgaccg
     3001 ccggctcgac gcgcgccacg tcgccgacgc tgacgcgcac gccgcgcgag gtcgtcagcg
     3061 ggagctcgag cagctcgtcg cgccggatgc gcgggagcat caacacgacg tcgcgctcct
     3121 cgtcgccggt ggtcagcacg gtcgagcgca ggccgtcgag cgaagcctcg agcgtcgcgg
     3181 tcacgagcgt cagatcgacg ccgagtccgt cggccagagt gcggtcgagc accacgcgca
     3241 gctcgggcgg cccgccttcg aacgacgaac gcacgttcca caactcgggt cgcgccgcca
     3301 ttgcctcgct caaccggtcg gcggccagcc gcaggtcgga cagcgcctgg cccgacacct
     3361 cgacgacgat cggcggaccg gaggtgccga gcgcctgcga cagcgcggac gagccgacct
     3421 cccacgaggc ctcgatgccg gccagctcgg cgacggccgg cgcggcggag gccacgacct
     3481 gaccggcggt cgcgccgccc gccttcagac ggacgacgac ccgcgccgtg ttctcctcgg
     3541 tctgctcgga gcggatgaag cggtcgtcct ccggcagacg gccgacctcg gacagcatcg
     3601 cgacgaggtc gtcgccgctg gcctggcgca ggatctgctc gaccgcctcg accgtgcgtg
     3661 cggtcgactc gacgcgttgg cccgggtagc cgaccagacg tacggagaac tgccgcgggt
     3721 cggacggcgg cagcagctcc gagccgaggc cgcgcagcag gaacaacgcg cccgccgtca
     3781 gccccagcgc gacgccgacg acgacgagcg ggcggccgag caagcgatga acgatgcgct
     3841 cgatccgctc gacacccggg tcgaccgggc gggcggtccc ctgcggcaac agccagcgcg
     3901 acaacgccgg gatcaacagc accgcggcga cgagcgacac caccagcgag acgacgacgg
     3961 tgaacgacag cccggtgacc agccggccgg ccagcccgcg gacgaagacg atcggcagga
     4021 agaccgcgca ggtggtcagc gtgctggcca ggatcgcccc gccgacgtcc gcggtgccgc
     4081 gggcgacggc gtcctcgccg gactcgccgc tggccagccg gcggtagatg ctctcgacga
     4141 cgacgatcgc gttgtcgacc agcatgccgg cgccgagcgc cagaccaccc agcgtcatga
     4201 tgttcagtgt ctggtcaccg aagcgcatca ggaacaacgt cccgagcagc gacaccggca
     4261 cggtggtggc gacgatcagc gtcggcccgg ccgcacgcag gaacagcgcc agcacgagca
     4321 tcgccaggcc gatgccgatc agcgccccgc gctgcacgtc cgcgatcgcg tcctcgacca
     4381 gcgccgcctc gtccgccacc acgtgcacgt cgattcccgg gaggtccccg cgcaggctgc
     4441 ccagcgcttc gcgcaccgtc cgcgatacct cgaccgtgtt ggcgcccgct tccttgtaga
     4501 tcgacaagcc gacaccctcg cgcccgtcga cgcgcaccag gtgggtgatc tccgcgtcct
     4561 cgacgcgaac ccgtccgagg tcgctgacgc gcaccggcac gcgccgcccg tcgtcgtcct
     4621 gaccgaatcg aacgacgacc tcacccacgt cgctcgcttc gcggtaccgc gccagcccgc
     4681 ggacgaggaa gaccttccct ccctcctcca gcgtgccggc gttgatgtcg acgttggccg
     4741 cgcgcaggcg ttcctcgagc tcgggcagcg cgacgccgta ggcctcgagc ttgtagcgat
     4801 cgacccgcac ctggacctgt ttctcgcggc cgccggtgac gcgcacctcg gccacgcccg
     4861 gcagccgttc gagcgtggtc gcgacctgcc ggcgcgcgat gcgctgcagc tcggcgagat
     4921 cggggccgcc ctcgtcggtc aacaggccga tggtcagcac ggcggcctga cgcgggtcga
     4981 aatgccgaac caggatctcg tcgacgtccg ggtcggagcg gatcggaccg accgccttct
     5041 cgacgtcgac cagcgcgaag tccatctcgg tttcccactc gaagacgacg gtggcgatga
     5101 tgcggccggt gcgcgcgacc tggttgatct cgcggatccc ggtcaccgcg aacagacgct
     5161 gctcgacctg ctcgccgtag attcgctcca tctcggccgg tgggcggtcg cccgagcgga
     5221 tcgacacgac gaccgtcggc gattccagat ccggcagcag gtcgagcggc aggctacgcc
     5281 aggcggtcca gccgaggatc acgacggcgg tggccaccac cgtcaccgcc accggtcgtc
     5341 gtgtcgccag acgagcgatc agctgcatga gggttcacgc gccggtggga cggacgctcg
     5401 taccgtcggt caacgtctcg agcccgcgca cgacgatccg ctcaccgggc tcgacaccct
     5461 ggcggatctc gatcacctcg tcatcgccca gaccgacgcc gacgtcacgc tggttgacct
     5521 tctggccgtt cagcacgaag acgaccttgc gacccccgcg ctcggtgacc gcttcccgcg
     5581 ggacgaccgc cacgtcctcg cgccgctccg cgatcagcgt cacctcgacg aacatccccg
     5641 gacgcagccg tccctcggga ttctgcacgg cgacctcggc gcgcagggtc cgcgtcaccg
     5701 gatcgagcgc cggcgccagc cgaagcaccc gcccgtcgaa cgcctggtct tcccaggcat
     5761 ggtgccggat gcgggccggc atcccgccga cgacgcgcgc gacgtcgcgc ccgacgagat
     5821 cgacgtcggc gatcaggctg gtcgccggcg cgatctgcgc gacgacgaat ccctgcgtca
     5881 cgagctgacc gtcggccaac ggcgttccgt tctcgtcgcg cgccagccgc aggatgacgc
     5941 cgccgatcgg ggtcacgagg ctcgagcggg cttcggtcag caggctgcgc tcccattcga
     6001 tcttcgactc ggcaagcgcg gtctcgacct ggcggaacag ctcctcgctc aacaggcctt
     6061 cgtcgaacag ctctttcttg gactcgtaat cgcggcgcgc ggcctcgtaa cgctggcggg
     6121 tggcgtcggt gccggccgcc agacgcacct cctcaccggt gatcaccgcg acgacctggc
     6181 cggcgtcgac gcgcgaaccc tcggccagcc gccggccggc ggggtcggac gcgatggcca
     6241 acgcaccgcc cgtgtcggcg cgcagcgaca cgatctccag tgagcgcaat gtacccgtcg
     6301 cgacgattcg atcctcgacc gtgccggtcc gcaccggctc gacaaagacg gggacgcgga
     6361 actcgaccgt gcggtcgttg ccctgcgccc cgcaaccggc ggagagcagc aacgaaccga
     6421 cggcgagcgc ggcgaggcgc cggcggcagg tcatcgattg ggcgcctccg ccgatgcgag
     6481 cgttccgtcg gcggccgcgc cgaccggcgt gaagcggacg gcgtcggctt ggacgtagtc
     6541 gccgtcgg
//
LOCUS       DASPGN010000355        10950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_531006, whole genome shotgun sequence.
ACCESSION   DASPGN010000355 DASPGN010000000
VERSION     DASPGN010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10950
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531006"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VD788_10105"
     CDS             complement(<1..284)
                     /locus_tag="VD788_10105"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXV76660.1"
                     /translation="MSEQDPTRPPDDLLVAALGAALFASDEPVPVSELSEAFGGVPPA
                     EIEGAIDRLREQLDRSSSGLRVEPVAGGFQLSTTPAVGPWVRQFFRQRNRA"
     gene            complement(281..1093)
                     /locus_tag="VD788_10110"
     CDS             complement(281..1093)
                     /locus_tag="VD788_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HXV76661.1"
                     /translation="MNHTIQPDSGRAGEISAALAEDAYRIRVARFEGPLDLLLHLVRS
                     NEIDILDIPIVEVTAQYNAHLELMREMNLEIAGEYLVMAATLMHIKSRLLLPPDPSAE
                     EDEEDPRAELAQELLEYQRFKQAAENLHAMDSRRTLVWTRTSVPPEFLGEELLAVDLF
                     DLLKAFRGLLGRLGEEARLQLKRDTVSVADKIHWLGELLERRKTIDLLELLTDLPTRL
                     DRIATFLAVLEMVRLQIVVVFQRRAFDEIRLAKVIGSDDDEPARRTVEERAD"
     gene            complement(1152..1748)
                     /locus_tag="VD788_10115"
     CDS             complement(1152..1748)
                     /locus_tag="VD788_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76662.1"
                     /translation="MPTLAKTVLSLALLAASAVCLAATPAAPYDLIVHVVWGDESVGP
                     ESLREQVERSVVRWLDAAACYHSLARFDDDHASPNDDVSDLLFTIEISNLEVREDWEV
                     SIAERTSPDQQPGETDDDLVATVGFDVDFVLQLLPERQALRQRSFRHEKSYRPRFGED
                     ARESVRDEAIDDLARSARSFVCNRRKKLAGEIARATAD"
     gene            complement(1777..2538)
                     /locus_tag="VD788_10120"
     CDS             complement(1777..2538)
                     /locus_tag="VD788_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXV76663.1"
                     /translation="MSKSPFQNFVVRFRNGESVFSAGDIGATMFIVQSGSVRLHHDAD
                     GEQRELGVMEKGDFFGEMSILEGLPRTHSATAVDDTELIEINSTTFDKMIKSNIEIAI
                     RMLRKLSIRLREAERRLNSRGLAGSSSGARIPTTIGETADSAGASITGIRLEAEEGAT
                     AFPLTGPETLIGRYDPVTELKPDVDLTDLDLKRSVSRRHARILETSDGFALVEEVGAL
                     NGTFVNGTRLAAGEPHPIREGDTLGFGMVRVVFRG"
     gene            complement(2602..3486)
                     /locus_tag="VD788_10125"
     CDS             complement(2602..3486)
                     /locus_tag="VD788_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXV76664.1"
                     /translation="MIRAAAVWTLFALAGAATAAPTLDDERNLFEWSSVAPMVVAGSS
                     LGENGRYTELRVIRTLRGDPRADDVIRVDVKTANRMRDRLIEPKALRLDLGQDYVLLL
                     EGEQRGSGRPYYTLVRGVDGALELPAEGREALLHALATFVEIQDTADDRLWWERFSRL
                     IEDPNPALVQTALEQFVRYRRGDPQLVPALVPLLDHPKIGIRADSAKLIGQILERHPD
                     PHAIPDDAAVRAELVARATSDEAPPVRVAAVEALARYPADLVDEVLERIARTDPEQQV
                     RYAAELVRMEHRRNGQNR"
     gene            complement(3543..5024)
                     /locus_tag="VD788_10130"
     CDS             complement(3543..5024)
                     /locus_tag="VD788_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76665.1"
                     /translation="MVLFNYSTRELTAKIVYYGPGLCGKTTNLQYIHGTLPDNVRGKM
                     LSLATKTDRTLFFDFLPIDLGQIRGMKTRVQLYTVPGQVFYNETRKLVLKGADGIVFV
                     ADSQSTMLGANIESFKNLEENLKAHGMTLADMPHVLQFNKRDLPKLSSIEELNAAINR
                     FNAPFYESVATTGIGVQDTLKAITKLVLLHLTRKYDPKTAPAAKTTQRPAVGLTGAPP
                     LAVEAPAGPAPSLSQTMQMPAVDPRDAFAPGGTHPRRVPLSSDPAAFDPLAASPAPSE
                     PAFEDREIQDLVDEVQEVSTLSAAGVSLAPETPETSAWDAPAPSEPPDLSSGVAEAGG
                     GPSFEIDHGVDPDLAGAAASAARRSSFSDAPRRTPADSPPAWELGGDDGQPATEDAAP
                     GRWDLGSFDEPSRESETAPDGPPFWEIGEVDASAADRAPAGGLPTLTEVVSDVELFDG
                     TVDVARLTEGQEQELVVPVEIEQADRSVRRLKLSIRLRIDPVV"
     gene            complement(5158..6483)
                     /gene="der"
                     /locus_tag="VD788_10135"
     CDS             complement(5158..6483)
                     /gene="der"
                     /locus_tag="VD788_10135"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXV76666.1"
                     /translation="MRPLVAIVGAPNVGKSTLFNRLVGGRPSIVTDEPGVTRDRLYGE
                     VRRARRAFRLVDTGGLTPNTAAPFAEEIERQAGIAVDEAACLLLVVDGRGGLTATDRD
                     VASRLRRRGLPILLVANKIDDPAVERLALELFELGLGEPLPVSAEHGLGIAELLERID
                     AVLGPGDTIEDAEDDGSGPPIQVALVGRPNVGKSSLLNRLAGEPRALVSEVPGTTRDA
                     VDTILEFAERRFRLIDTAGIRRPSRVRLRAERFSVVRARHNIDRSDVVVLLLDATDEF
                     AAQDAHIAGYVADAFKPLIVAVNKWDLVGEREQAAKRMTADARQRLRFAKEAPIVLVS
                     AKTGQRVTRLLETAARLHALGGSEIPTAEVNRWLQQVSGGLGEGAPGSIRFFYATQTG
                     TRPLTFLVFCNDPTRVHFSIRRRMENTLRERFSLGGVPIRLRFRARRGQ"
     gene            6640..6909
                     /locus_tag="VD788_10140"
     CDS             6640..6909
                     /locus_tag="VD788_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXV76667.1"
                     /translation="MIKADIINRVAEDARITKVKAVEAVEAVFDAMKGAMKRGERIEL
                     RGFGVFQVKPRKKGIGRNPRTGREVRIPPGKTIRFKPGKNLRNLG"
     gene            7100..7894
                     /locus_tag="VD788_10145"
     CDS             7100..7894
                     /locus_tag="VD788_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXV76668.1"
                     /translation="MATARTTADLLRPQAIVEAIVAGLPYGFWTIAILAAHEMGHYLA
                     CRHYGIPVTLPFFIPGPPLLVGSFGALIRIRGRIPDRKALFDVAAAGPLSGFAVALPV
                     LLVGVAVAEPSPAASGDAGLWLGDPLVVSLYAWLVPRAGTSEANQLIGAGWVGMLVTS
                     LNLFPVGQLDGGHATYAISRRLHRALAWVSLAGMAVLLVYQVVALEQAPAYLIWFAIL
                     LWMRDRHPPLVDEAASIGAVRVVLAGLLLLIFVLSFIPIPLTVIDG"
     gene            complement(7881..10223)
                     /locus_tag="VD788_10150"
     CDS             complement(7881..10223)
                     /locus_tag="VD788_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HXV76669.1"
                     /translation="MPPRRPAIAPCAAFVVGAFVGLHGVGQAPLLPGALSALALLLAL
                     GAHARAPRLASFAVCASLAGAGAALSLAERSESAARLATCFPDGQRSIELDFSGRVIA
                     IAEPTFEGERLLLLAGRGTGRAGSCRLTVKLRIGRSPDASTRVVDRLDGGDRVRVWCR
                     LTRPRGLANRSAEADPSEPPSGGVDAWGSVKSARLVVLDGPGRAGVDRATGRLKREAR
                     RRLDLLLGPNGETRALAGAMLLGDRAMLHPNTQRSLRDSGLFHLCAISGLHVGIVMLL
                     SIALIERSRLSRPTRVLLALALLYGFGQFVGLRASVARATLAAAAAQAGRLFDRTGDP
                     LNTLCWIAALLVGCSPPLVGDLGFQLSATATAGILLALSPKVRVSPLRVSAAAYLATV
                     PLTAFHFGWVAPVALVANLVAVPLCTFVLGSGYLALLGCGVPFVGPLAGRISEWSGEA
                     MLGFARAVSTFEPGAFPVARPAAAVVAAFYFLAVAWAVARLRDARPTACRLLGCAGAV
                     CLAWIHLGPPPPATRELAAAVIDVGQGQAVIARVPGGGSLLVDAGGSHNRRFDPGERV
                     VLPFVLGFAGRRIEHLVLSHDHLDHVGGAAAIVDALEVGSLCLPPGFARSTRMKRLAD
                     TVVARGGALRLCIAGVRLVGERAVVLAPPRVAWGTSVNDRSVVIRIGSPGHRLLVPGD
                     LERAGEHALVHSAVSLTAEALVLSHHGSRTGSTRALLDRVAPRAALISCGHDNRFGHP
                     HAETIERVRVAPVTLWRTDLDGAIQLRESSGGWSIRATRR"
     gene            complement(10301..>10950)
                     /locus_tag="VD788_10155"
     CDS             complement(10301..>10950)
                     /locus_tag="VD788_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76670.1"
                     /translation="LPEDPTVTSVPPSVGVRTYAREIERRWARLQQRPRILSPKEWAL
                     ICDWYARAVPLQVIEEAMQAALDKSHADRPPRTLVYLAASVEEAWEVLVDGRRAQLHD
                     GSSRSDPADAARIWQERLLAEPRSSPLKALLERLLEDHGRGADPVELDARLDAELPAA
                     ISPDRLARVRAAVAGELEPFVERISAEVLAETARTAMLTRLRRELGLARLAPARG"
BASE COUNT         1677 a         3830 c         3810 g         1633 t
ORIGIN      
        1 gcccgattgc gctgtcgaaa gaactgacgc acccacgggc ccaccgcggg tgtggtcgag
       61 agttggaagc cgccggccac cggttcgacg cgcaagccgc tcgacgagcg gtcgagctgc
      121 tcgcggagcc gatcgatcgc cccctcgatc tcggcgggcg gcacgccgcc gaacgcctcg
      181 gacagctcgc tcacgggaac cggctcgtcg gaggcgaaca gcgcggcgcc gagcgcggca
      241 accaacagat cgtccggcgg gcgggtcggg tcttgctcgc tcaatcggct cgctcctcga
      301 cggtgcggcg agccggctcg tcgtcgtcgc ttccgatcac cttggccagg cggatctcgt
      361 cgaacgcccg acgttggaag acgacgacga tctgcagccg taccatctcg agcaccgcca
      421 ggaaggtcgc gatgcggtcg agccgggtcg gcaggtcggt aagcagctcg agcaggtcga
      481 tcgtcttccg acgctcgagc aactcgccca accagtggat cttgtcggcc accgaaacgg
      541 tatctcgctt gagctgcagg cgggcttcct cgcccaggcg gccgagcaga ccgcgaaacg
      601 ccttgagcag gtcgaacaga tcgaccgcca gcagctcctc gccgaggaac tctggcggca
      661 cactggtgcg ggtccaaacc agcgtccgcc tggagtccat cgcatgcagg ttctccgccg
      721 cttgcttgaa gcgctggtac tcgagcagct cttgcgccag ctcggcccga ggatcttctt
      781 cgtcctcctc ggccgacggg tccggcggca gcagcaggcg ggacttgatg tgcatcagcg
      841 tcgccgccat caccaagtac tcgccggcga tttcgagatt catctcccgc atcagctcga
      901 gatgcgcgtt gtactgcgcg gtgacctcga cgatcgggat gtcgaggatg tcgatctcgt
      961 tcgatcgtac gagatgcagc aggagatcga gcgggccttc gaatcgcgcg acgcggatgc
     1021 ggtaggcgtc ttcggcaagc gcggcagaaa tctcgcccgc tcggccgctg tcgggttgga
     1081 tcgtgtggtt cacgtcgatc tcactggctc cgggcgacga cccgccactc taacacgcgc
     1141 tcgatggacg atcaatcggc ggtggcgcgg gcgatctcgc cggccagctt cttccggcgg
     1201 ttgcagacga acgaacgcgc gctgcgcgcc agatcgtcga tcgcctcgtc gcggacggat
     1261 tcgcgtgcat cctcgccgaa acgaggccga tacgacttct cgtggcggaa gctgcgctgc
     1321 ctcagcgcct ggcgttcggg cagtagctgc agaacgaagt cgacgtcgaa tccgacggtc
     1381 gccacgaggt cgtcgtcggt ctcacccggt tgctggtcgg gcgaggtccg ttccgcgatg
     1441 ctgacctccc agtcctcgcg cacttcgagg ttgctgatct cgatggtgaa caacaggtcg
     1501 gaaacgtcgt cgttgggtga cgcgtggtcg tcgtcgaagc gtgccagcga gtggtaacag
     1561 gcggcggcgt cgagccagcg aacgacggag cgttcgacct gttcgcgcag gctctccggt
     1621 ccgacgcttt cgtcacccca gaccacgtgg acgatgaggt cgtagggggc ggcgggggtc
     1681 gcggcgaggc agacggcgct cgccgcgagc agcgccagcg acaggacggt cttcgcgagc
     1741 gtcggcatct ccccgacagt ctagcggaag gaagcctcag ccgcggaaca cgacccgcac
     1801 catgccgaag ccgagcgtgt ccccctcccg gatcggatgg ggttcgccgg ccgcgaggcg
     1861 cgtgccgttg acgaaggtgc cgttcagcgc cccgacttct tcgaccagcg cgaaaccgtc
     1921 gctcgtctcc aggatgcggg cgtgccggcg ggagacgctg cgtttgagat cgagatcggt
     1981 gagatcgacg tccggcttga gctcggtgac gggatcgtag cggccgatca gcgtctccgg
     2041 gccggtcagc ggaaaggcgg tcgcgccctc ctcggcctcg aggcggattc cggtgatcga
     2101 cgccccggcc gaatcggcgg tctcgccgat ggtcgtcggg atccgcgctc cggacgagct
     2161 gccggccaag ccccgcgagt tcagccgccg ttcggcctcg cgcaggcgga tcgacagttt
     2221 gcgcaacatg cggatggcga tctcgatgtt gctcttgatc atcttgtcga aggtcgtgct
     2281 gttgatctcg atcagttccg tgtcgtcgac ggcggtcgcc gagtgcgtgc gcggcaagcc
     2341 ttcgaggatc gacatctcgc cgaagaagtc gcccttctcc atcacgccga gctcccgctg
     2401 ctcgccgtcg gcgtcgtggt ggagacgcac ggagccggac tgcacgatga acatcgtggc
     2461 cccgatgtcg cccgcggaga agaccgactc cccgttacgg aaacggacga cgaagttctg
     2521 gaacgggctc ttcgacatct tcccggatga tcataggttc cggattcggc ggaagtcaat
     2581 tcgacgtaac ctggcccgaa ctcagcggtt ttggccgttg cgccggtgct ccatccgcac
     2641 gagctcggcg gcgtagcgca cctgctgctc ggggtcggtt cgggcgatcc gctcgaggac
     2701 ctcgtcgacc aagtcggccg ggtagcgcgc caaggcctcg accgccgcaa cccggaccgg
     2761 gggcgcttcg tcgctcgtcg cccgcgcgac gagctccgct cggacggcgg cgtcgtccgg
     2821 aatcgcgtgt ggatcgggat ggcgctcgag gatctggccg atcagcttcg cgctgtccgc
     2881 ccggatgccg atcttcgggt ggtcgagcag cggcacgaga gccgggacca gctgcggatc
     2941 gccccggcga tacctgacga actgctcgag cgccgtttgc acgagcgccg ggttcggatc
     3001 ttcgatcagc cgggagaatc gctcccacca cagccggtca tcggccgtgt cctggatctc
     3061 gacgaaagtg gcgagcgcgt gcagcagcgc ctcccggcct tccgccggca gttcgagggc
     3121 gccgtcgaca ccgcggacca gcgtgtagta cggacggccg ctcccgcgct gttcaccctc
     3181 gagcagcagc acgtagtctt ggccgagatc gagccgcagg gccttcggct cgatcagccg
     3241 gtcgcgcatg cggttggcgg tcttgacgtc gacgcggatg acgtcgtccg ctcgcggatc
     3301 gccgcgcagc gtgcggatga cgcgcagttc ggtgtagcgc ccgttctcgc cgagactgga
     3361 accggcgacg accatcggcg ccaccgacga ccactcgaac aggttgcgct cgtcgtcgag
     3421 cgtcggggct gcggtcgcgg cgccggcgag cgcgaacaac gtccagacgg cggcggctcg
     3481 gatcacccta tgagcatgca cgacgaccgc ggcggctgcc agccgtgggc cgcggcgccg
     3541 cgtcacacga cggggtcgat ccgcagccgg atcgacagct tcagccgccg caccgaacga
     3601 tcggcctgct cgatctcgac cggcacgacg agttcctgct cttggccctc ggtcaagcgc
     3661 gccacgtcga ccgtaccgtc gaacaactcc acgtccgaga cgacctcggt cagcgtcggg
     3721 agtccgccgg ccggggcacg gtcggccgcc gaagcgtcga cctcgccgat ctcccagaac
     3781 gggggtccgt cgggcgccgt ctcgctctcc cgggaaggct cgtcgaacga accgagatcc
     3841 cagcgcccgg gcgcggcatc ctccgtggcg ggctggccgt cgtcgccgcc caactcccac
     3901 gccggcggcg aatcggcggg ggtgcggcga ggcgcatcgc tgaacgacga gcgccgcgcg
     3961 gcgctcgccg cggcgccggc cagatcgggg tcgacgccgt gatcgatctc gaagctcgga
     4021 ccaccgccgg cctccgcgac gccgctcgac aaatcgggcg gctcgctcgg cgcgggcgca
     4081 tcccacgccg acgtctcggg ggtctcgggc gcgagcgaga cgccggccgc ggacagcgtg
     4141 gagacctcct ggacctcatc gaccagatct tggatctccc ggtcctcgaa cgcgggctcg
     4201 gacggagccg gtgacgccgc cagcgggtcg aacgccgccg gatcactcga cagcggcacg
     4261 cggcgcgggt gtgtgccgcc cggggcgaac gcgtcacgcg gatcgacggc cggcatctgc
     4321 atcgtctgcg agagcgacgg cgccggaccc gccggggctt cgaccgccag cggcggcgcg
     4381 ccggtcaggc cgaccgcggg ccgctgggtc gttttggccg ccggcgcggt cttcgggtcg
     4441 tacttccggg tgaggtgcag cagcacgagc ttggtgattg ccttcagcgt gtcctgcacg
     4501 ccgatgccgg tcgtcgcgac cgattcgtag aacggcgcgt tgaaccgatt gatggcggcg
     4561 ttcagctcct cgatgctcga cagcttcggc agatcgcgct tgttgaactg gagcacgtgc
     4621 ggcatgtccg ccagcgtcat gccgtgcgct ttgaggttct cctcgaggtt cttgaacgac
     4681 tcgatgttgg cgccgagcat cgtcgactgc gaatcggcga cgaacacgat gccgtcggca
     4741 cccttcagca ccagcttgcg cgtttcgttg tagaacacct gacccgggac ggtgtagagc
     4801 tggacccgcg tcttcatccc gcgaatctgg ccgagatcga tcggcaggaa gtcgaagaac
     4861 agcgtgcggt cggtcttggt cgcgagcgat agcatcttgc cgcgtacgtt gtccggcagc
     4921 gtcccgtgga tgtactgcag gttcgtggtc ttcccgcaaa gccccggccc gtaatagacg
     4981 atcttcgcgg tcaactcacg cgtcgaatag ttgaacagta ccatcggtga ttcccccgac
     5041 cctgcagccg attcccctcg gtccggaaac tccaggacga ccgtcgcgga acatatatcc
     5101 cggccccggc agggtcaagc gcgtcgcacc cgacgggcgg cccgacacgt cgggccgtca
     5161 ttggccacga cgtgcgcgga aacgcagccg gatcggcacg ccgccgagcg agaaccgctc
     5221 ccgcagcgtg ttctccatcc ggcggcggat cgagaagtgc acccgggtcg gatcgttgca
     5281 gaagacgagg aaggtcagcg ggcgagttcc ggtctgcgtg gcgtagaaga accggatcga
     5341 gcccggcgcg ccctcgccca ggccgccgct cacctgctgc aaccaacggt tgacttccgc
     5401 cgtcgggatc tcgctgccgc cgagcgcgtg caaccgtgcg gcggtctcga gcaaacgcgt
     5461 cacgcgctgg ccggtcttgg cgctgaccag cacgatcggc gcctccttgg cgaagcgcag
     5521 acgttggcgt gcgtccgccg tcatccgctt ggccgcctgc tcgcgctcac cgacgagatc
     5581 ccacttgttg acggcgacga tcagcggctt gaacgcgtcg gcgacgtaac cggcgatgtg
     5641 cgcgtcctgg gccgcgaact cgtcggtcgc atcgaggagc agcaccacga cgtccgagcg
     5701 atcgatgttg tggcgcgcgc gaacgacgct gaaccgttcg gcgcgcagcc gcaccctgga
     5761 ggggcggcgg attcccgccg tgtcgatcag acggaagcgc cgctcggcga actcgagaat
     5821 cgtgtcgacc gcatcgcggg tcgtgccggg gacctcgctg accaaggctc gtggctcgcc
     5881 ggccagccga ttcaacagcg acgacttgcc gacgttcggc cggccgacga gcgcgacctg
     5941 gatcggcggc cccgacccgt cgtcctcggc gtcctcgatc gtgtcgcccg gtccgagcac
     6001 cgcgtcgatc cgctcgagca actcggcgat gcccagaccg tgctcggccg acaccggcag
     6061 cggctcgccg agacccagct cgaacagctc gagcgccagc cgttcgacgg ccggatcgtc
     6121 gatcttgttc gcgaccagca ggatcggcaa accccgacgg cggaggcggc tcgcgacgtc
     6181 ccgatcggtc gcggtcagac cgccccggcc gtcgacgacg agcagcaggc aggccgcctc
     6241 gtcgactgcg atgccggcct gccgctcgat ctcctcggcg aacggcgccg cggtgttggg
     6301 cgtcagcccg ccggtgtcaa ccagacggaa cgcccgccga gcgcgtcgga cctccccgta
     6361 cagccggtca cgggtcacgc ccggctcgtc ggtcacgatc gacgggcggc cgccgacgag
     6421 ccgattgaac agcgtgctct tgccgacgtt cggcgctccg acgatggcga ccagcggtct
     6481 cacggtttcc gaccctagca cgcgtcgcac cgccgggcca gccgaggcga gagctaagtt
     6541 catatttgag atggatttag cgagatgcgt cgcttgacag ctctggccgg tccgcgtaca
     6601 ctgaccgcct tttgggaggc tcttaggagg tccgccccga tgatcaaggc cgacatcatc
     6661 aaccgcgtcg ccgaggacgc caggatcacc aaggtcaagg ccgtcgaagc ggtcgaggcc
     6721 gtcttcgacg cgatgaaggg ggcgatgaag cgcggggaac ggatcgagct gcggggcttc
     6781 ggtgtcttcc aggtcaagcc gcgcaagaaa gggatcggcc ggaatccccg caccggtcgg
     6841 gaagtccgga tcccgccggg caagaccatt cgtttcaagc cgggtaagaa cctgcgcaac
     6901 ctgggctgac ccactggaac cgacgatccg cccgtatcga gccccgctcg ccgacgacgg
     6961 ctggcccacg cgcgaccgtc cgacgcggtc cgaccctccc ccggtccgcc ggtcgggccg
     7021 accgctgttc cacgtcgcgc tgttcgtcac caccttcctc acgctgtcgt ggaccggtgg
     7081 tctgttctgg gaagggctga tggcgaccgc gcggacgacc gccgaccttc ttcggccgca
     7141 ggcgatcgtc gaggcgatcg tcgccgggct gccctacgga ttctggacga tcgcgattct
     7201 cgcggcccac gagatgggtc actacctcgc ctgccgccac tacggaatcc cggtgacgct
     7261 tccgttcttc atcccgggac cgccgctttt ggtcggttcg ttcggtgcgc tgattcgcat
     7321 ccgcggccgc atcccagacc gcaaggcgtt gttcgacgtg gccgcggccg ggccgctttc
     7381 gggctttgcc gtcgcgctgc cggtgctcct cgtcggtgtg gcggtcgccg aaccgtcgcc
     7441 ggcggcgtcg ggggatgcgg ggttgtggct cggcgatcca ctggtcgtct cgctctacgc
     7501 ctggctcgtg ccgcgcgcgg gcacgagcga agccaaccag ctgatcggcg cggggtgggt
     7561 cggcatgctc gtcacctcgc tcaacttgtt ccccgtcggc cagctcgacg gtgggcatgc
     7621 gacctacgcg atctcccgcc gattgcaccg ggcgctggcc tgggtctcgc tggccgggat
     7681 ggccgtgctg ctggtctacc aggtcgtcgc gctcgagcag gcaccggcct acctgatctg
     7741 gttcgcgatc cttctgtgga tgcgtgaccg ccaccccccg ctcgtcgacg aagcggcctc
     7801 gatcggggcg gtgcgcgtgg tgctcgccgg gctcctgttg ctgatcttcg tgctgtcgtt
     7861 cattccgatc ccgctgaccg tcatcgacgg gtagcgcgaa tcgaccagcc gccggaggac
     7921 tcgcggagct ggatcgcgcc gtcgaggtcg gtgcgccaca acgtaaccgg ggcgactcgc
     7981 acccgctcga tcgtctcggc gtgcgggtgt ccgaagcggt tgtcgtgacc gcaggagatc
     8041 aacgcggcgc gcggtgcgac gcggtcgagc agggcgcgtg tgctgccggt ccggctgccg
     8101 tggtgcgaca gcaccagcgc ctcggccgtg agcgacaccg cggagtggac gagcgcgtgc
     8161 tcccccgccc gctcgagatc gcccggtacc agcagccggt gccccggcga gccgatccgg
     8221 atcacgaccg agcggtcgtt gacgctcgtt ccccaagcca cgcgcggcgg agcgagcacc
     8281 accgcccgtt cgccgaccaa gcgaacaccc gcgatacaga gtcgcagcgc tccgccgcgc
     8341 gcgacgaccg tgtcggccag ccgcttcatt ctcgtggaac gcgcgaaccc gggcgggagg
     8401 cagagcgatc cgacttcgag cgcgtcgacg atcgcggccg cgccgccgac gtggtccagg
     8461 tggtcgtggc tcagcacgag gtgctcgatc cgccgcccgg cgaagccgag tacgaagggc
     8521 aggacgaccc gttcacccgg atcgaaacgc cgattgtggc tgccgccggc gtcgacgagc
     8581 aacgagccac cacccggcac tcgggcgatg accgcctgcc cctggccgac gtcgatcacg
     8641 gccgccgcca gctcgcgggt cgcaggcggg ggtggtccga ggtggatcca cgccagacag
     8701 accgcaccgg cgcagccgag cagccggcac gcggtcggtc tcgcgtcgcg aaggcgggcg
     8761 acggcccagg cgaccgccag gaagtagaac gccgcgacca ccgccgccgc gggccgtgcg
     8821 acggggaacg cccccggctc gaacgtcgag acggcgcggg cgaaccccag catcgcctcc
     8881 ccgctccact cgctgatgcg acccgcgagc gggccgacga acggcacccc gcagcccagc
     8941 agcgcgaggt atcccgagcc gagcacgaac gtgcacaacg gcaccgccac cagattggcg
     9001 acgagggcga ccggggcgac ccagccgaag tggaatgccg tcaacgggac ggtggcgagg
     9061 taggcggcgg ccgagacacg cagcggagaa acgcgcacct tcggcgagag cgccagcagg
     9121 atgccagcgg tcgcggtcgc gctcagctgg aagccgaggt cgccgaccag cggcggcgag
     9181 cagccgacga gcaatgccgc aatccagcac agcgtgttca gcgggtcgcc cgttcggtcg
     9241 aacaaacgcc cggcctgggc cgccgccgcg gccagggtcg cacgggccac cgacgcgcgt
     9301 aatccgacga actggccgaa gccgtagagc agcgcgaggg cgagcagcac ccgtgtcggc
     9361 cgcgagagtc gcgagcgttc gatcagcgcg atcgacagca acatgacgat gccgacgtgc
     9421 agaccgctga tcgcgcagag atggaacaac cccgagtcgc gcagcgaacg ctgtgtgttc
     9481 gggtgtagca tcgcgcgatc gccgagcagc atcgcgcccg cgagagcccg tgtttccccg
     9541 ttcgggccga gcaacaggtc gaggcggcgc cgcgcctcgc gtttgagccg gcccgtcgcc
     9601 cgatcgacac cggcgcgccc gggcccgtcg agcacgacca gtcgcgcgct cttgaccgag
     9661 ccccacgcat cgaccccacc gctcggcggc tcggacggat cggcttctgc ggaacggttg
     9721 gccagcccgc gggggcgggt caggcggcac cacactcgca cgcgatcacc cccgtcgagt
     9781 cggtcgacca cccgggtcga ggcgtcgggc gaccggccga tgcggagctt gaccgtcagc
     9841 cggcacgagc cggcgcgtcc ggtcccccgg ccggcaagga gcagcagccg ttcgccctcg
     9901 aacgtcggtt ccgcaatcgc gatgacgcgg ccgctgaagt cgagttcgat cgagcgttgg
     9961 ccgtcgggaa aacaggtggc gagccgcgcg gcgctctcac tccgctcggc gagcgacagc
    10021 gcggcgcctg cgcccgccag cgaggcgcag accgcgaacg aggcgagacg tggtgcccgc
    10081 gcatgcgcgc cgagcgccaa cagcagcgcc agtgccgaca gggcgccggg cagcagcggt
    10141 gcttggccga cgccgtgaag cccgacgaac gcgccgacga cgaacgcggc gcacggcgcg
    10201 atggcgggtc gtcgcggtgg cactcgccac ccttcgtggg gcgcgggatc ctcgggtcaa
    10261 tgtagcgcgt gcgcgcggca cgtgccgtcc gaaccccttc tcagccgcga gccggggcca
    10321 gtcgcgccaa ccccagttcg cggcgcagcc gggtcagcat cgccgtgcgg gcggtctcgg
    10381 ccaggacctc ggcggagatg cgctcgacga atggctcgag ctccccggcg accgcggcgc
    10441 gcacgcgcgc gagacggtcg ggcgagatcg cggcgggcag ctccgcgtcg aggcgcgcgt
    10501 cgagctcgac cggatcggcc ccgcgcccgt ggtcttcgag cagtcgctcg agcagcgcct
    10561 tcagcggtga agagcggggc tcggcgagca ggcgctcctg ccagatacgt gccgcgtcgg
    10621 cggggtccga ccgcgacgac ccgtcgtgaa gctgggcgcg gcgaccgtcg accagcacct
    10681 cccacgcctc ctcgaccgac gccgcgagat agaccagcgt ccgtggcggc cgatcggcgt
    10741 ggcttttgtc cagcgcggcc tgcatcgcct cctcgatcac ctgcagcggg acggcgcggg
    10801 cataccagtc acagatcaac gcccactcct tgggagagag gatccgcggg cgctgctgca
    10861 ggcgagccca gcggcgctcg atctcgcgcg cgtaggtccg gacgccgaca cttggcggga
    10921 cgctcgtcac cgtcgggtct tcaggaaggt
//
LOCUS       DASPGN010000356         4651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_63049, whole genome shotgun sequence.
ACCESSION   DASPGN010000356 DASPGN010000000
VERSION     DASPGN010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63049"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..636
                     /locus_tag="VD788_10160"
     CDS             253..636
                     /locus_tag="VD788_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76671.1"
                     /translation="MVTAAQRPAWSELDEPQRRELDLQYLRELSMVSRPGLTAAGIQA
                     YLDYRHERPERAITTFEEGQIDRLLQRYVDRSHLPDPVALEEHRGRLERFGAARLHLT
                     NAETYVAIMVTLNLLFVIALLVVLM"
     gene            702..3788
                     /locus_tag="VD788_10165"
     CDS             702..3788
                     /locus_tag="VD788_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV76672.1"
                     /translation="MAGDAQWPTIRAVVERALARPPAERAAFVAAACRDDEALHRQVE
                     LMLESVDESFIELDETRALDMQAVAAAADPLIGRSLAHYRITDKVGQGGMGAVYRAWD
                     DKLGREVAIKVLPAEFAEDPRRLERFEREARMLASVNHPNIATIHDVDESHGVHYLVL
                     EMVEGEGLDRRIARAPLAFDEAVPVFIQIADALEAAHEKGIIHRDLKPANVHVTPRGQ
                     VKVLDFGLAKALEALPVGPASTGAAQRSAPRQTRAGAIVGTVTYMSPEQARGKPVDKR
                     TDFWSFGCTLYEALAGRPPFRGDSLFETVAAIIEREPDWAALPADTPARTRRLLERCL
                     DKDPERRVAAAADVRAALAPRERRTWRPWLVMSALVLVLLGLGLWRVWRDRAAVSVTA
                     TPKVAVTGVTNLSGRSQLDWLEEGLASLVRDRLAESRHVAVVSRSRWETIARSAGDSR
                     DLSRLAARSGIDFVVSGELLSTPAGLVWTARVTDAAAGVDLVARRIEGLTETSLLDSS
                     NRVALLAKQAMKIPHTETVEVFAADFAAEHPGAYESYLAGLEFFGDFAYDDAERSFRS
                     ALELEPGYHVARYRLAHVLWLTGRTEQAQATLAAIPNDAELTPRERLYIEGARALLER
                     DWTAAKQAYRSLLEQYPYELEAREFLAEVHDMEYDDEAALEQLRILAEQEPENPAVWA
                     NLGQTHLRVGAFDEAEAALDTYLALAPAEPLGFTVLGELNRARRRFDVAVRHFEHALE
                     LSPGFPPAMLGLATTSALAGDRARAEELWQRLVDDETEQPGYRIDAAFELSALRRAAG
                     RFEQSLEPLARLAPLIEREGFREALALSTEGLIRVELGQLDAAGERIALAIERSPSVP
                     TRYLFARGWLELARGDADAVRATAEEIRGHTLPPADPDRTEDKAAAFLDGMAALAAGD
                     AARAVEATRRAVALEGHAYASYELGLARALRAADRHEEAVKLAAAAAAAPDPVEPRLD
                     LELDRVRALLVEAEIRDEMNDRSAARRLARAFLERFAAAPGDHPDVVRARALARKG"
     gene            complement(3785..>4651)
                     /locus_tag="VD788_10170"
     CDS             complement(3785..>4651)
                     /locus_tag="VD788_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76673.1"
                     /translation="ERYIEFTSERFDSHDALDDNTGNFIVRGRLVDDAYVEGALLNKP
                     YLRGSDGRVRPLPRPQTGGELIDGFGYGADDDLVGLVLLADLGGARVFDADFDLVPGV
                     VRNLSGFLNSVSVQLLDKSGKTALYASMEALAGVFEPIALFDLDVADPTIDYLRRVES
                     GPLEGFRFDDEPQDDDELLELLVASYAPVEFDLRAVVVAGVAPEFIHDEDGDGRFTAD
                     DVASMGYELLSNPVELAIREQFDLSITETVEGRTCPSRSLIFVDLDGNGEDGEVPCSG
                     TGGARRIRRTPR"
BASE COUNT          652 a         1672 c         1692 g          635 t
ORIGIN      
        1 cctcgctgtc ccagcaggag aagggcggat aggtcgagac gaagtagttg ccgacccgcg
       61 gcgaggcggc gtcgagggcg gcgcccggtg tgcgctcagc catgacgcac cccggcgtgc
      121 tgacgttcgg cgaggtcgag catctcgagt cagtgtaggt gcgtgccgcg ggccgccgca
      181 gacggcaaag acgacagcga gggtcttcaa tcggcacgac cgagccggcg gcgggcgatg
      241 tacgtttgaa gcatggtcac cgccgcccag cgcccggcct ggagcgagct cgacgagccg
      301 cagcgtcgcg agctcgacct gcagtacctg cgcgagctgt cgatggtcag ccggccgggc
      361 ctgaccgccg cggggatcca agcctatctc gactaccggc acgagcgccc ggagcgcgcg
      421 atcaccacct tcgaggaagg ccagatcgac cggctgctgc agcgttacgt cgatcgttcg
      481 cacctgcccg atcccgtcgc gctcgaggag caccgcgggc ggctcgagcg cttcggcgcg
      541 gcgcgactgc acctgacgaa cgcagagacc tacgtcgcga tcatggtcac actcaatctg
      601 ctgttcgtga tcgcgctgct cgtcgtgctg atgtagcacg gcgcgcgacc ccgccgcgcg
      661 cggttgaccg ccgccggact gtgccggaca atgccccggt catggcgggc gacgcgcagt
      721 ggccgacgat tcgcgcggtg gtcgagcggg cgctcgcgcg tccgccggcc gaacgcgccg
      781 ccttcgtcgc cgcagcctgc cgcgacgacg aggcgctcca ccggcaggtc gagctgatgc
      841 tcgagtcggt cgacgagagc ttcatcgagc tggacgagac ccgcgcgctc gacatgcagg
      901 ccgtcgcggc cgccgccgac ccgctgatcg gccgctcgct ggcccactac cgcatcaccg
      961 acaaggtcgg tcagggcggt atgggcgcgg tctaccgcgc ctgggacgac aagctcggcc
     1021 gcgaggtggc gatcaaggtg ctgccggccg agttcgccga agatccgcgg cgactcgaac
     1081 ggttcgagcg cgaggcgcgc atgctggcct cggtcaacca cccgaacatc gcgacgatcc
     1141 acgacgtcga cgagtcgcac ggcgtgcact acctcgtgct cgaaatggtc gaaggcgagg
     1201 ggctcgaccg gcggatcgcg cgtgcgccac tcgcgttcga cgaggccgtg ccggtcttca
     1261 tccagatcgc cgacgcgctc gaagccgcgc acgagaaggg gatcatccac cgcgatctca
     1321 agccggccaa cgtccacgtc acgccgcgcg gccaggtcaa ggtgctcgac ttcgggctgg
     1381 ccaaggcgct cgaggcgctg cccgtcggcc cggcctcgac cggtgctgcg caacgctcgg
     1441 cgccccggca gacgcgcgcc ggcgcgatcg tcggcacggt gacctacatg agtcccgagc
     1501 aggcgcgcgg caagccggtc gacaagcgga ccgacttctg gtcgttcggc tgcacgctgt
     1561 acgaggcgct ggccggccgg ccgccgttcc gcggcgacag cctgttcgag acggtcgccg
     1621 cgatcatcga gcgcgagccg gactgggcgg cgttgccggc cgacaccccg gcgcggacga
     1681 ggcgcctgct cgagcgctgt ctcgacaagg acccggaacg ccgcgtcgcc gccgccgcgg
     1741 acgtgcgcgc cgcgctcgcg ccacgcgagc ggcggacgtg gcggccgtgg ctcgtgatgt
     1801 cggcgcttgt gctcgtcttg ctcggtctcg gtctgtggcg cgtctggcgc gaccgcgcag
     1861 ccgtcagcgt cacggcgacg cccaaggtcg ccgtgacggg cgtgaccaac ctcagcggcc
     1921 gatcgcagct cgactggctc gaggaagggc tggccagcct ggtccgcgac cgtctcgccg
     1981 aatcgcgcca cgtggcggtg gtgtcgcgat cacgctggga gacgatcgcc cgctcggccg
     2041 gcgacagccg cgacctcagc cggttggcgg cccgttccgg catcgacttc gtcgtatcgg
     2101 gcgagctgct gtcgacgccc gccgggctgg tgtggaccgc ccgcgtgacc gacgccgcgg
     2161 cgggcgtcga cctcgtcgcg cgccgcatcg aggggctgac cgagacgagc ctgctcgact
     2221 cgagcaatcg ggtcgcgctg ctggccaagc aggcgatgaa gatcccgcac accgagacgg
     2281 tcgaggtctt cgccgccgac ttcgccgccg agcaccccgg ggcctacgag tcgtacctcg
     2341 ccggtctcga gttcttcggc gacttcgcct acgacgacgc cgagcgctcg ttccgctcgg
     2401 cgctcgagct cgagccgggc taccacgtcg cgcgctatcg gctggcgcac gtgctgtggc
     2461 tgaccggccg caccgagcag gcgcaggcca cgctggcggc gatcccgaac gacgccgagc
     2521 tgacgccgcg cgagcggctg tacatcgaag gcgcgcgcgc gctgttggag cgggactgga
     2581 cggcggccaa gcaggcctac cgatcgttgc tcgagcagta cccgtacgag ctcgaggcgc
     2641 gcgagttcct cgccgaagtc cacgacatgg agtacgacga cgaggccgcg ctcgagcagc
     2701 tgcggattct cgccgagcag gagccggaga acccggcggt gtgggccaac ctcggacaga
     2761 cccacctgcg ggtcggcgcg ttcgacgagg ccgaggcggc gctcgacacc tatctcgcgc
     2821 tggcgccggc cgagccgctg ggcttcaccg tgttgggcga gctgaatcgg gcgcgccggc
     2881 gattcgacgt cgccgtccgg cacttcgagc acgcgctcga gctgtcgccc ggcttcccgc
     2941 cggcgatgct cggcctggcg accacctcgg cgctcgccgg cgatcgcgca cgcgccgagg
     3001 agctgtggca gcggctggtc gacgacgaga ccgagcagcc cggctaccgc atcgacgcgg
     3061 cgttcgagct gagcgcgctg cgtcgcgccg ccggccgctt cgagcaatcg ctcgagccgc
     3121 tcgcgcgtct cgcgccgttg atcgagcgcg aggggtttcg cgaggcgctg gcgctgtcga
     3181 ccgaggggct gattcgcgtc gagctcggcc agctcgacgc ggcgggcgag cggatcgcgc
     3241 tcgcgatcga gcgctcgccg tcggtgccga cgcgctacct gttcgcgcgc ggctggctcg
     3301 agctggcgcg cggcgacgcc gacgccgtgc gcgccaccgc cgaggagatc cgcggccaca
     3361 cgctgccgcc cgcagacccc gatcgcaccg aggacaaggc cgcggccttc ctcgacggga
     3421 tggccgcgtt ggccgcgggc gacgccgcgc gcgccgtcga ggcgacgcgc cgggcggtcg
     3481 cgctcgaagg ccatgcctac gcgtcgtacg agctcggcct ggcgcgcgcg ctgcgcgcgg
     3541 cggatcggca cgaggaggcc gtcaagctgg ccgccgccgc cgccgccgcg cccgatccgg
     3601 tcgagccgcg tctcgatctc gagctcgatc gtgttcgcgc gctgctcgtc gaagcggaga
     3661 tccgcgacga gatgaacgac cgctccgcgg ccaggcgcct ggcgcgggcg ttcctcgagc
     3721 gcttcgccgc ggcgcccggc gaccaccccg acgtcgttcg cgcgcgcgcc ctggcgcgca
     3781 agggctagcg cggcgtgcgg cgtatccggc gcgcgccgcc ggtgcccgag cacgggacct
     3841 cgccgtcctc gccgttgccg tcgaggtcga cgaagatcag gctgcgtgac gggcaggtgc
     3901 gcccctcgac ggtctcggtg atcgacaggt cgaactgctc gcgtatcgcc agctcgaccg
     3961 ggttcgacaa cagctcgtag cccatcgacg ccacgtcgtc ggccgtgaaa cgcccgtcgc
     4021 cgtcctcgtc gtggatgaac tccggcgcga cgcccgcgac gacgaccgcc cgcaggtcga
     4081 actcgaccgg ggcgtaggag gcgacgagca gctcgagcag ctcgtcgtcg tcctgcggct
     4141 cgtcgtcgaa gcggaagccc tcgagcgggc cgctttcgac ccgtcgcagg tagtcgatcg
     4201 tcgggtcggc gacgtcgaga tcgaacagcg cgatcggttc gaacacgccg gccagcgcct
     4261 ccatcgacgc gtagagcgcg gtcttgccgc tcttgtcgag cagttggacc gagaccgagt
     4321 tgaggaagcc ggagaggttg cggacgacgc cgggcacgag gtcgaagtcg gcgtcgaaca
     4381 cgcgcgcgcc gccgaggtcg gccagcagca ccagcccgac caggtcgtcg tcggcgccgt
     4441 agccgaagcc gtcgatcagc tcgccgccgg tctgcgggcg cggcagcggt cgcacgcggc
     4501 cgtcgctgcc gcgcaggtag ggcttgttca gcagcgcgcc ctcgacgtag gcgtcgtcga
     4561 ccagccgccc gcgcacgatg aagttgccgg tgttgtcgtc gagcgcatcg tgggagtcga
     4621 atcgctcgga ggtgaactcg atgtaccgtt c
//
LOCUS       DASPGN010000357         5264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_63473, whole genome shotgun sequence.
ACCESSION   DASPGN010000357 DASPGN010000000
VERSION     DASPGN010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63473"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VD788_10175"
     CDS             complement(<1..1068)
                     /locus_tag="VD788_10175"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HXV76674.1"
                     /translation="MSGGLRRLGPLVVLGLYLSAAPAWAQQPFTVETVVEPDSGLSDT
                     APFHIVLHITGQQPPGIVQPEPFPLIDGLSVVNGPNTNRRFFWRDGEASATYQLSYVV
                     IAERPGRYTVPSLGVEIDGRDYRTEPLTFEVGRGRDGSRGVSPSGTIGQPRQQADPVF
                     FEIKLGEDEVWVGQPVPLSVTLYSAYRVTGVVFGETPGFPDFWVEQVEVDADAERFQT
                     RIDGRLYTGYPVDRRMLIPLAPGAFTIEPYVGQVQVRQSGAGDRFDFFSFGRTHTVVR
                     KTPELEVHVKPLPPGQPAGFSGAVGRFTIDVRADRDRARVNDAVALRATVEGEGSLQS
                     VAPPTLVEPTGAKLFEPTVTES"
     gene            complement(1065..1799)
                     /locus_tag="VD788_10180"
     CDS             complement(1065..1799)
                     /locus_tag="VD788_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76675.1"
                     /translation="MTRVLLVPLVLASLLSPFGGKAHRKTEEANRHYELGEFGEALAD
                     YTEAQVVAPESPRLHYDVGNVLYRQGDYEGAAEAFTRALVTAPDELAPLAAFNLGNAR
                     FQQREYGDAADAYRRALEGRPDDADAKRNLELALRALEQQEQQPQQDSQQDPSEDPQP
                     QDQPQQGPGQDAEPPESDSGERGGEPPPKTGEERSESEAQPSGMTPEQAERLLDSLAD
                     EERDNLERERRRKAAAARERRLEKDW"
     gene            complement(1796..2836)
                     /locus_tag="VD788_10185"
     CDS             complement(1796..2836)
                     /locus_tag="VD788_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV76676.1"
                     /translation="MRFAAPEMLWLALSVPAMVLIAVWLGRRRRRALERFAGASEFVA
                     RFDGTVSLHRRAVKLLLLALALVGVIVAAARPQWGTRLEAVTRRGVDVVVLLDHSLSM
                     AAEDVKPNRLFRANQAIDSLLVRLAGDRVALVTFAGEATLLCPLTLDHSAVRLFLDAV
                     EAESTQVPGTALARALELSLEAFGESRSVSADESRAVLLLTDGEDHEGGIDEALRDLE
                     RAGVAVYTVGVGTTRGAPIPLADEAGMHTGYKKDPDGRVVTTRLEEPLLERIALESGG
                     RYYRATAAQIEVDEITRALTGLDTQEFGEVLRARYEERFQVPLAVALLALTLEALIGD
                     RRRAVEQGRSAG"
     gene            complement(2841..3839)
                     /locus_tag="VD788_10190"
     CDS             complement(2841..3839)
                     /locus_tag="VD788_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV76677.1"
                     /translation="MMIRLATPWVLLGIPLVVGAVWWMARRRATSDARLELPQAESRI
                     PLTLSPWVRLDRALPWVRAVALVLLVVSIARPQAGEQLEDVSTHGVDIVVALDVSGSM
                     RAEDFTPRNRLEVARRTIERFVNGRPTDRIGLVAFAAIAATRSPLTQDHEMLKSFLGQ
                     VDFAPADQDGTAIGLGLATAVNRLRESRAESRVVVLVTDGVNNAGQIGPRAAAQAAEA
                     LGVRVYTVGVGSEGRVPVPVDMGPLGRRVVLREMELDEALLEEIAATTDGQYFRATDP
                     EGLQRVFEQIDELEKTEIESRVRVLYSELFSWTLLPAIGLLLFERLLLVTRLRRLP"
     gene            complement(3836..4816)
                     /locus_tag="VD788_10195"
     CDS             complement(3836..4816)
                     /locus_tag="VD788_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76678.1"
                     /translation="MSRAAAFPLVAGLVLATLAGRPAAAETDVRLTVKPTEATVGQRL
                     DVELVVELDSGVTVETPSIGPRLGPFTVFGGRWEGPADDGARRRYRWLAQISAYRTGA
                     LEIPPIRLSVATGGQPVEIASEALTVDVRSVLEPPEGEQPLDIADIKPPVSIPPDYRP
                     LWLALTLVGVLAAAALALWWVQRRYAGKLAAVPIDDDLFRRVPPHEWIYRELQRLLEE
                     KLAERGEVDRFYAELSHALKRYLEGRYRVDLLERTTEEARSQLEQAGLDQPVIERVVE
                     FLAECDRVKFARERPASDRWKVAIEQVYEIVDRTTPAESTVEPGRQQGAA"
     gene            complement(4821..>5264)
                     /locus_tag="VD788_10200"
     CDS             complement(4821..>5264)
                     /locus_tag="VD788_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76679.1"
                     /translation="LAINPAGRGTRIGHALEYLQRVVRKRAVAFLISDFQDTGYERTM
                     RVVAQKHDLVAIAVSDPREQSLPDVGLISVEDPETGRRGLIDTGSAAARRAYAEAAAR
                     ARTELRRSVRRSGVDLLELSTGESYELPLVRFFHERARRAAMAGG"
BASE COUNT          787 a         1915 c         1780 g          782 t
ORIGIN      
        1 cgactcggtc acggtcggct cgaacagctt ggcgccggtc ggctcgacca gcgtcggcgg
       61 cgcgaccgac tgcagcgacc cttcgccctc gaccgtcgcg cgcagtgcga ccgcgtcgtt
      121 gacccgcgcg cgatcacgat cggcgcggac gtcgatcgtg aatcggccga cggccccgct
      181 gaagcctgcg ggctgtccgg gcggcagcgg tttgacgtgg acctcgagct cgggtgtctt
      241 gcgcacgacg gtatgcgtgc gaccgaagct gaagaagtcg aagcggtccc ccgccccgct
      301 ctgcctgacc tgcacttgtc cgacgtacgg ctcgatggtg aacgcacccg gtgcgagcgg
      361 gatcagcatc cgacgatcga cgggatagcc ggtgtagagc cgcccgtcga tgcgcgtctg
      421 gaagcgctcc gcgtcggcat cgacctcgac ctgctcgacc cagaagtccg gaaagcccgg
      481 cgtctctccg aacaccacgc cggtgacgcg gtaggccgaa tagagcgtga ccgacagcgg
      541 aaccggctgg ccgacccaca cttcgtcctc accgagcttg atctcgaaaa agaccgggtc
      601 cgcctgttgc cggggctggc cgatcgttcc gctcggtgac accccgcgcg aaccgtcccg
      661 tccccggccg acctcgaagg tcagcggctc ggtgcggtag tcgcgaccgt cgatctcgac
      721 gccgagcgac ggcacggtgt acctgccggg gcgctcggcg atcaccacgt aggacagctg
      781 gtaggtggcg ctcgcctccc cgtcacgcca aaagaaacga cggttggtgt tcggcccgtt
      841 gacgacgctc aagccgtcga tcagcggaaa cggctcgggt tgtacgatcc ccggcggctg
      901 ctggccggtg atgtgcaaca cgatgtggaa cggcgcggtg tcggagagcc ccgagtccgg
      961 ctcgacgacc gtctcgacgg tgaacggctg ctgcgcccag gccggcgcgg ccgacaggta
     1021 cagcccgagc acaaccagcg ggccgagccg acggagcccg ccgctcacca gtccttctcc
     1081 agacgccgct cgcgggccgc ggccgccttg cggcggcgtt cccgctcgag gttgtcccgc
     1141 tcctcgtccg cgagactgtc gagcagccgc tcggcttgct cgggggtcat cccgctcggc
     1201 tgggcctccg actccgaccg ttcctcgccg gtcttcggcg gtggctcgcc gccccgttcg
     1261 cccgaatcgg attcgggtgg ttcggcgtcc tgacccggac cctgctgagg ctggtcctgc
     1321 ggctgcgggt cctcgctcgg atcctgctgc gaatcctgct gcggctgctg ttcttgctgc
     1381 tcgagcgcgc gcagcgccag ctcgaggttg cgtttggcgt cggcgtcgtc cggccggccc
     1441 tcgagcgcgc gccggtaggc gtcggccgcg tcgccgtatt cgcgctgttg gaagcgggcg
     1501 ttccccagat tgaacgccgc cagcggtgcc agttcgtcgg gagccgtgac caacgcgcgg
     1561 gtgaacgcct ccgccgcgcc ttcgtagtca ccctgacggt acagcacgtt gccgacgtcg
     1621 tagtgcagtc gcggggattc gggtgcgacg acctgtgcct cggtgtagtc ggcgagcgcc
     1681 tcgccgaact ctcccagctc gtagtgacgg ttggcttcct cggtcttgcg gtgcgccttg
     1741 ccgccgaacg gcgagagcag cgacgcgagg acgagcggca cgaggaggac gcgtgtcatc
     1801 cggcactcct tccctgctcg acggcgcgcc gccggtcgcc gatcagcgct tcgagcgtca
     1861 gcgccagcaa cgcgacggcc agcggaactt ggaatcgctc ctcgtagcgg gcgcgcagga
     1921 cctcaccgaa ctcctgggtg tcgagaccgg tgagcgcccg cgtgatctcg tcgacctcga
     1981 tctgcgcggc ggtcgcgcgg tagtagcgcc cgcccgattc caacgcgatg cgctcgagca
     2041 acggctcctc gaggcgggtc gtgacgacgc gaccgtcggg atccttcttg tacccggtgt
     2101 gcatgccggc ctcgtccgcc agcggaatcg gcgcgccacg cgtcgtgccg acgccgacgg
     2161 tgtagaccgc cacgccggcc cgctcgagat cgcgaagggc ctcgtcgatt ccgccttcat
     2221 ggtcctcgcc gtcggtcaac aacaggaccg cgcggctctc gtccgccgag accgaccggc
     2281 tctcgccgaa cgcctcgagc gacagctcga gcgcccgcgc cagtgccgtg ccggggacct
     2341 gcgtcgattc ggcctcgacc gcgtcgagaa acagccgcac cgccgagtga tcgagggtca
     2401 gcgggcatag cagcgtcgcc tcgccggcga aggtcaccag cgccacccgg tctccggcga
     2461 gacggaccag caacgaatcg atcgcctggt tcgcgcggaa caaccggttc ggcttcacgt
     2521 cttcggcggc catgctcaac gagtgatcga gcagcacgac gacgtcgact ccgcgccgcg
     2581 tcaccgcctc gagccgcgtg ccccactgcg ggcgcgccgc ggcgacgatc acaccgacca
     2641 acgccagcgc cagcaacagc agcttgactg cccggcgatg caggctcacc gtgccgtcga
     2701 agcgcgccac aaactcgctg gcgccggcga accgctcgag tgcgcgccgg cgtctccggc
     2761 cgagccagac ggcgatcagc accatcgccg gaaccgacaa cgccaaccac aacatctctg
     2821 gcgcggcgaa tcgcatcttc tcaaggaagc cttcgcagac gcgtgacgag cagcagccgc
     2881 tcgaacaaca gcaggccgat cgccggcaac agggtccatg agaacagctc gctgtacagc
     2941 acccgcacgc gactctcgat ctcggtcttt tccagctcgt cgatctgttc gaacacgcgc
     3001 tgcaacccct cggggtcggt cgcacgaaaa tactggccgt cggtcgtcgc cgcgatctcc
     3061 tcgagcaggg cctcgtcgag ctccatctcg cgcaacacga cgcggcggcc caacgggccc
     3121 atgtcgaccg ggaccgggac gcgcccctcg ctgccgacgc cgacggtgta gacgcgaacg
     3181 ccgagtgcct ccgccgcctg tgccgccgcc cgcggaccga tctgtcccgc gttgttcacc
     3241 ccgtcggtca ccagcacgac gacccggctc tcggcccgcg actcgcgcag gcggttgacc
     3301 gcggtcgcca gaccgagccc gatcgcggtg ccgtcctggt cggcgggggc gaaatcgacc
     3361 tggcccagga acgacttgag catctcgtga tcctgggtca gcgggctgcg cgtcgccgcg
     3421 atcgcggcga acgcgacgag gccgatacgg tccgtcggac ggccgttgac gaagcgctcg
     3481 atcgtacgcc gggccacttc gaggcgattg cgcggcgtga agtcctccgc gcgcatcgag
     3541 cccgacacgt cgagggccac gacgatgtcg accccgtgcg tcgagacatc ctcgagctgc
     3601 tcgcccgcct gcggacgggc gatcgagacg accagcaaca ccagcgccac cgcgcggacc
     3661 cacggcagcg cccgatcgag gcggacccac gggctgaggg tcaacgggat gcggctttcc
     3721 gcctggggca gctcgaggcg tgcatcggag gtggcccgtc ggcgcgccat ccaccagacg
     3781 gcgccgacga cgagcgggat cccgagcaac acccagggtg tcgccaaccg gatcatcatg
     3841 ccgctccttg ctgccgaccg ggctcgaccg tcgattcggc cggcgtcgtc cgatcgacga
     3901 tctcgtagac ttgctcgatc gcgaccttcc agcggtcgga cgcgggccgt tcgcgggcga
     3961 acttcacccg gtcgcattcg gcgaggaact cgacgacgcg ctcgatcacg ggctggtcga
     4021 gccccgcttg ctcgagctgc gaccgcgcct cctcggtcgt gcgctccagc aagtcgacgc
     4081 ggtatcgccc ctcgaggtag cgcttgagcg cgtgcgacag ctcggcgtag aatcggtcga
     4141 cctcgccgcg ctcggcgagc ttctcctcga gcaaccgctg caattcgcgg tagatccact
     4201 cgtggggcgg cacgcgacgg aacaggtcgt cgtcgatcgg cacggcggcg agctttccgg
     4261 cgtagcgccg ctggacccac cacaaggcga gcgcggcggc ggccagcacc ccgaccagtg
     4321 tgagcgcgag ccatagcggc cggtagtccg gcggaatcga caccggcggt ttgatgtcgg
     4381 cgatgtcgag cggctgttct ccctccggcg gctcgagcac gctcctgacg tcgacggtca
     4441 gcgcttcgct cgcgatctcg accggctgcc cgccggtcgc gaccgacaag cggatcggtg
     4501 ggatttcgag cgcaccggtc cgataggcac tgatctgcgc cagccaccga tagcgccgcc
     4561 tcgcgccgtc gtcggccgga ccttcccacc gtccgccgaa gaccgtgaac gggccgagcc
     4621 ggggtccgat cgacggggtc tcgactgtca caccgctgtc gagctcgacg acgagctcga
     4681 cgtcgagccg ctggccgacg gtggcctcgg tcggcttgac ggtgagccgg acgtcggtct
     4741 ccgcggcggc cgggcggccg gcgagcgtgg cgagaaccaa gccggcgacc aacgggaagg
     4801 cggcggcgcg ggacaacgtg tcagcctccc gccatcgccg ctcgccgagc gcgctcgtga
     4861 aagaagcgga ccaacggcag ttcgtacgac tcacccgtcg acaattcgag caggtcgacg
     4921 ccgctgcgac ggacgctccg gcgcagttcc gtacgcgcgc gcgccgccgc ctcggcgtag
     4981 gcccgccgcg ccgccgcgct gccggtgtcg atcagtcccc gtcgccccgt ctcggggtcc
     5041 tcgacgctga tcagtccgac gtccggcagg ctctgctcgc gcggatcgga gacggcgatc
     5101 gccaccaggt cgtgcttctg cgcgacgaca cgcatcgtcc gctcgtagcc cgtgtcctga
     5161 aaatcggaga tcaggaaggc gacggctcgt ttgcggacga cgcgctgcag gtactcgagg
     5221 gcgtgcccga tccgcgttcc gcgccccgcc gggttgatcg cgag
//
LOCUS       DASPGN010000358        11204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1703681, whole genome shotgun sequence.
ACCESSION   DASPGN010000358 DASPGN010000000
VERSION     DASPGN010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11204
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1703681"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1222
                     /locus_tag="VD788_10205"
     CDS             149..1222
                     /locus_tag="VD788_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76680.1"
                     /translation="MVNLDRLAVETGGRFTERTNDLQLAYARAQRDRACTYTLGFYVE
                     ADDESRSRSLSVRVKRPGMRAIAPQRYVFRSDDEKRESLLRAAWIAPEMFQTGVVRAH
                     AYPLRPADKGAWEALLAVGFTVPLGDSAGAAVRRRFGAMLYQGSRVVHRFSRTVTLQP
                     DHSGVDSTPVVTFLERVRLPPGSYSLTAVVSDPLDTRPHAVKVPIAVEPIPRRELFLS
                     PPILGRRSGPNLVVIGGGSGGDDQLGSSASFEPMLVQQVDATVDAVAVSQACMLGSPK
                     HALEKKTRILRTLHRADGGAVGRLDPLPLTLDGEDKIRCQNLVDVIPTASIVDGEYVF
                     HVELAAGADDASSGRRVRFVVSD"
     gene            1342..3303
                     /locus_tag="VD788_10210"
     CDS             1342..3303
                     /locus_tag="VD788_10210"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV76681.1"
                     /translation="MSHVLLLVFVTGILASDWPQWGGPQRDFTAAEAGIELSAFPTPG
                     PRVVWDKPLGPGYAAIVTHGDRLVTAYRRGQFDVVVCLDRERGDTRWEHAYEAPVPRD
                     GVDLDTSYGEGPNSTPLLADGRVYTLGLTGVVHALDLDDGTVAWSHDLRREYEVPIPY
                     FGHAASPVRHGDSVIFIAGGALAFDLETGELRWHNRSFGASYASPLIVDGEHGAVLVA
                     AVAGEIVGLDPSDGALLWRHEHANQHRTILSSPILGADGRLFVSAYWVGSLGLVLGAD
                     GRSAAEAWRAPQVQLARTNAVRVGDWIFGFHRSVLSAVNAVTGEIGWRHRGLRQGNLV
                     RLGGDRFLLLDAIGNLTLASLTPEGAVVHDETQLFHGRAWTVPTIVGTTLYARDLERI
                     VAVDLRGTAETPSVARTEPAPGRAELAEPAFREAIAGLARAARREDAAGLAAARGAFA
                     AWRDRAGVAHLAHYYLAFAAWRQAVGASDTQALAAVDAAVEHLGEAIRLRKEFADAHA
                     LLATLYAMYYRLDPQRAAVIGPLADEHLDLALLFGGDHPRVVLTDGLRRIDRPVEYGG
                     DRRAGLARVREALELFERESTDESGIEPGWCHGLAWSWYGEALMRGTEEEREPARRAF
                     ERALEIDADLATARRMLAELTEQAPGGQR"
     gene            3332..4405
                     /locus_tag="VD788_10215"
     CDS             3332..4405
                     /locus_tag="VD788_10215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXV76682.1"
                     /translation="MTAVRWGAITACWLVWGLFYASRLRLVISDVSWAETLSYSMPDA
                     VLWAALTPIPLALARRFPIRADRWWAHIPLHLAAAGVVAGVHGLLDATLNVVFGSPLS
                     FGEMLLHLLRFGVHTNVMLYFAIVGVSQFFARSQRLRERERQASELRAQLAQARLDAL
                     QMQLRPHFLFNALNTVSGLVEQDVAAGRRVLRQLGDVLRMILQRRDRQEVSVREELDL
                     VRAYLAIESARFGERLTVQVDVAEKTLGLAVPSFLLQPLVENAVTHAVARRIRGGRID
                     LRAWCGDGSRLVVSVEDNGAEDASAPVESGQGIGLTNARARLRQLYGDEQELRVLRNE
                     RGGWTVTVSIPARSLADAMRRSA"
     gene            4402..5166
                     /locus_tag="VD788_10220"
     CDS             4402..5166
                     /locus_tag="VD788_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HXV76683.1"
                     /translation="MKLLRALVVDDEPAARQALRSLLEHVADLRVIGECASGAEAVES
                     VHRLRPDLLFLDVEMPDGNGFDVLQQLAPGHLPAVVFVTAFDHYAADAFRVDAIDYML
                     KPFDAERLAETVSRVRRRLARGGPTPSEWADALRALADRGRSLERVAVLDGDASIVIK
                     TSQIRWLESQANYLRLHVGDRSYLARGTMRSMEQRLDATRFVRIHRTAIVNLDHVRQL
                     RPIGHGDLQLVLADGTELSVSRRYRSGLERLVERLA"
     gene            5206..6186
                     /locus_tag="VD788_10225"
     CDS             5206..6186
                     /locus_tag="VD788_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV76684.1"
                     /translation="MKALALVLCAACAVPPSVAQQPKYKNLQVFPADVSEAELDEAML
                     ENLRGLGLRRLAGEGCLYCHVGDLETPRSEWNYASDAKPAKQKARVMMRMVRAINEEY
                     LSKLSPRAAPAFQVTCTTCHEGRLDPRPLPDVLWAVYETDGIDGTTRRYQELRDGYFG
                     AAAYDFRVHVLSGIATRMADRGAIDDAIRLAEVNVEVYPQEPAAKRAWVALTLERTID
                     ADGVDAALSQLARLEPTLAEGVVTPSLLDSLAWRLNRSERETQGHALIEANFSKFPGE
                     YTPLESLVFVRSNSGRKDEALALLEGWLDEHPDHARARRVWINLTQTPTD"
     gene            complement(6211..7077)
                     /locus_tag="VD788_10230"
     CDS             complement(6211..7077)
                     /locus_tag="VD788_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76685.1"
                     /translation="MRYAHVSTVLSLVFSLLVPAAAAHDARSAFEIHGVTQDFIDRMA
                     ALGYPDLDDDRLVAFKIHGVSERFVEEMRDVGLPRLEPDQLIAFRIHGVDRRFVEEMN
                     GLALGRFSPDTLIGFRIHGVSPGFIEQMRALGLELDADDAMAFRIHGVTAEFIDEMAE
                     LGFAGLDSGDLIAFRIHRIDRAFIDEMADLGLTGLTVDQALRLRIHQVDERFRDEMAE
                     LGLGNFTADRLVAMRILDVDEELIRELRGGPVGPDAVRRILGERSCGRRDCFDDGLWN
                     ALIEVVGDLSGL"
     gene            complement(7132..7683)
                     /locus_tag="VD788_10235"
     CDS             complement(7132..7683)
                     /locus_tag="VD788_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76686.1"
                     /translation="MASITGLWLPILLSAVFVFVASSIVHMVLRYHWTDWSRLPGEDK
                     ARAALTGLPPGNYRMPYCSSMADLKDETFVGKMKEGPNALVIGLPTGPPTMGKQLVQW
                     FVYTLAVGVVTAYVASRTMDAGADYLAVFRVAGTVAFLTYAGASPAGSIWVGMKWSTT
                     LKSLFDGLIYGAVTGGTFGWLWP"
     gene            7790..8284
                     /locus_tag="VD788_10240"
     CDS             7790..8284
                     /locus_tag="VD788_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76687.1"
                     /translation="MSLTVTAAVGLILAIKVPQTRAEFVDAVAAGRGATKVETVVVER
                     DIDGVYQTLAERSAACLDVLVERSGFVGTHWEVSSSDYNPTLERIDDGTAAFALQVEH
                     RPRGVGHKPPPGGLYVFAAEIKALDDSRTEVVLYRPTMGFKKIAASFRAWLDGDDAGC
                     PKLR"
     gene            8339..8566
                     /locus_tag="VD788_10245"
     CDS             8339..8566
                     /locus_tag="VD788_10245"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HXV76688.1"
                     /translation="MKTTLNIDDSVMARLKQEAARSGRTMSELVETALRQLFRSRRSR
                     EELPPIPTFASGGALVDLADRDALYQAMEGR"
     gene            8581..9012
                     /locus_tag="VD788_10250"
     CDS             8581..9012
                     /locus_tag="VD788_10250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HXV76689.1"
                     /translation="MDTNVLVYAADRDSPHHAACLARLESWRSANDAWFVTWSILYEF
                     LRVVSHPRVLRKPWTVPQAWSFVEAVLASPGLEVLAETERHADVARRTIRELPHLAGN
                     LLHDAHTAILMREHGVRRIYTRDTDFHRFGFLEPLDPLAAG"
     gene            complement(9035..9406)
                     /locus_tag="VD788_10255"
     CDS             complement(9035..9406)
                     /locus_tag="VD788_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76690.1"
                     /translation="MRSPRFVALVVSVVLLGSWTTDGGELRTVTTTGSIPASAERVLR
                     AFVDADDLVRRLGRLRRGTDPARLGRCGRGSGATAAEGRAPHRGYRSGGDWNWMHDTV
                     VEGWRLVLDDMQRWFRLERRE"
     gene            complement(9450..10109)
                     /locus_tag="VD788_10260"
     CDS             complement(9450..10109)
                     /locus_tag="VD788_10260"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXV76691.1"
                     /translation="MRTRIFLVRHGETTSAAEDRFAGSTDVELAETGRRQARALGRRL
                     AHEALAVAYTSPMTRTRETARLILDGRDVETTVVPELREIDHGQWEGKSKDEVRRMWT
                     EEFRAWEADPFTFAPSGGETGLEVLNRALPRLLAIAERHVGASVLVVSHKATIRLLVG
                     HLLGFALRGFRDRLGQDPCALNVLDVQRGGEARLLLFNDVSHYEDLPGPVGSRLSPRW
                     P"
     gene            complement(10145..10603)
                     /locus_tag="VD788_10265"
     CDS             complement(10145..10603)
                     /locus_tag="VD788_10265"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXV76692.1"
                     /translation="MASSTNTIRLHRVLRAPAERVYRAFLEPEALVKWIPPHGFTGKV
                     HEIDARVGGGYRMSFTNFSTGTSHSFGGRYVELEPHRRIRYTDRFDDPNLPGEMQVAI
                     MLDEVSCGTELRIVQEGVPAVIPAEACYLGWQESLSMLARLVEPEIPDGL"
     gene            complement(10716..>11204)
                     /locus_tag="VD788_10270"
     CDS             complement(10716..>11204)
                     /locus_tag="VD788_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76693.1"
                     /translation="DYALAESEMCRALYAASGFGPRDWRFAETLDELGLIAFRLRDYE
                     VAERLQGAAIAEMLLAVGPHGEPLADDDGSKRVAIRPDCRSGIEVYTRRLGWIHEHVE
                     PSVTISEIRDAPWVVLTAGYIPLDAASVHRLDWLVSQYLLVENVAAAELLTEFQRRIR
                     GE"
BASE COUNT         1679 a         3961 c         3831 g         1733 t
ORIGIN      
        1 cgccgcgatc ggtgccgtct cccgctgctc gatctacccg gtcgacgtga gagggctgta
       61 cgacgaagga cgctcgttca aactggacac gttcggctga gggtgaagcg gcggcagccg
      121 cgcgggagtt gcccgcgcca acgtcccgtt ggtcaacctc gaccgactcg ccgtcgaaac
      181 cggcgggcgg ttcaccgagc gcaccaacga cctgcagctg gcctacgccc gggcgcagcg
      241 cgatcgggcc tgcacctaca cgctcggctt ctacgtagag gccgacgacg agagccggtc
      301 gcgttcgctg tcggtacggg tcaagcgccc gggtatgcgc gcgatcgccc cccagcgcta
      361 cgtctttcga tcggacgacg agaaacgcga gtcgctgctg cgcgccgcct ggatcgcgcc
      421 cgagatgttc cagaccggag tggtgcgcgc ccacgcctac ccgttgcggc cggccgacaa
      481 gggcgcctgg gaggcgttgc tcgccgtcgg cttcaccgtg ccgctgggcg acagcgccgg
      541 cgcggcggtg cggcgccggt tcggcgcgat gctctaccaa ggaagccgcg tggtgcaccg
      601 cttctcgcga acggtcaccc tgcagcccga tcactccggg gtcgattcga cgccggtcgt
      661 caccttcctc gaacgggtcc ggctgccccc cgggagctac tcgttgacgg cggtcgtctc
      721 cgacccgctc gacacgcggc cgcacgcggt caaggtgccg atcgcggtcg aaccgattcc
      781 gcgacgggag ctgttcctct cgccgccgat cctcggccgg cgctccgggc cgaacctggt
      841 ggtgatcggc ggcgggtccg gcggcgacga ccagctcgga tcgtcggcca gcttcgagcc
      901 gatgctggtc cagcaggtcg acgcgaccgt cgacgccgtg gccgtgagcc aggcctgcat
      961 gctcggctcg ccgaaacacg ctctcgagaa gaagacgcgg atcttgcgca cgctgcaccg
     1021 ggccgacggc ggcgccgtcg gtcggctcga cccgctgccg ctcacgctcg acggcgagga
     1081 caagatccgc tgccagaacc tggtcgacgt gatcccgacc gcctcgatcg tcgacggtga
     1141 gtacgtgttc cacgtcgaac tcgccgcggg cgccgacgat gcgtcctccg gccggcgtgt
     1201 ccgcttcgtc gtctcagact gaagcccggc cgctcgtccc gacccccgac ccgcccgtcg
     1261 ccccgctcgt cccgctcgtc acttcgggca aaccgacgac ggcggtcggg cgttcgaacc
     1321 tccgacgaga ggaggtctcc gatgtcgcac gtcctgctac tggtcttcgt caccggcatc
     1381 ctcgcctcgg actggcccca gtggggcgga ccgcagcgtg acttcacggc ggccgaggcg
     1441 gggatcgagc tctccgcctt ccccactccc ggaccacgcg tcgtctggga caagccgctg
     1501 gggccgggct atgcggcgat cgtcacccac ggcgatcgac tggtgacggc ctaccgccgc
     1561 ggccagttcg acgtggtggt ctgtctcgat cgggagaggg gcgacacgcg atgggagcac
     1621 gcctacgaag cgccggttcc gcgcgacggt gtcgacctcg acacgtccta cggcgagggc
     1681 ccgaacagca cgccgttgct cgccgacggg cgggtctaca cgctcgggct caccggcgtg
     1741 gtccacgcgc tcgatctcga cgacggaacg gtcgcgtggt cccacgacct gcgtcgcgag
     1801 tacgaggtac cgatcccgta cttcggtcac gccgcgagcc cggtgcgcca cggcgacagc
     1861 gtcatcttca tcgccggtgg cgcgctggcg ttcgacctcg agaccggcga gctgcgctgg
     1921 cacaaccgtt ccttcggcgc gagctacgcc tccccgctga tcgtagacgg cgagcacggt
     1981 gcggtcctgg tggcggcggt cgcgggcgag atcgtcggtc tcgatccgtc agacggcgcg
     2041 ctgctgtggc gtcacgagca cgcgaaccag caccgcacga tcctcagctc gccgatcctc
     2101 ggtgcggacg gccgtctgtt cgtctcggcc tactgggtcg gcagcctcgg gctcgtgctc
     2161 ggtgccgacg gtcgatcggc ggccgaagcg tggcgggccc cgcaggtgca actcgcccga
     2221 acgaacgccg tgcgcgtcgg tgactggatc ttcggcttcc accggtcggt gctgagcgcc
     2281 gtgaacgccg tcaccggcga gatcggctgg cgtcaccgcg gactgcggca aggcaacctg
     2341 gtgcgcctcg gcggcgatcg cttcctgctg ctcgacgcga tcggcaacct gacgctcgcc
     2401 tctctcacgc ccgagggcgc ggtcgtgcac gacgagacgc agctgtttca cgggcgtgcc
     2461 tggacggtgc cgacgatcgt cggcacgacg ctctacgccc gcgatctcga acgcatcgtg
     2521 gcggtcgacc tgcgcgggac ggcggagacg ccgtccgtcg ctcgcacgga accggctccc
     2581 gggcgtgcgg agctggccga gcccgcgttc cgcgaggcga tcgccgggct ggcgcgcgcc
     2641 gcgcgtcgcg aggacgcggc cgggctcgcc gctgcccgcg gggcgttcgc ggcctggcgc
     2701 gatcgggccg gcgtggcgca tctggcccac tactacctcg cgttcgccgc gtggcggcag
     2761 gcggtcggcg cgagtgacac gcaggcgctc gcggcggtcg acgcggcggt cgagcatctc
     2821 ggcgaggcga ttcgcctgcg caaggagttc gccgatgccc atgccttgct ggcgacgctg
     2881 tatgcgatgt actaccgcct cgatccgcag cgcgcagccg tcatcggacc gctggcggac
     2941 gagcatctcg acctcgcgct gctgttcggc ggagatcacc cgcgcgtcgt gttgaccgac
     3001 gggctgcggc ggatcgaccg cccggtggag tacggcggtg accggcgggc gggcctcgcg
     3061 cgcgtccgcg aagcgctcga gctgttcgag cgcgagtcga ctgacgaatc ggggatcgag
     3121 ccgggctggt gccatggtct ggcctggagc tggtacggcg aggcgctgat gcggggtacg
     3181 gaggaagagc gcgagccggc gcggcgcgcc ttcgagcgcg cgcttgagat cgacgccgac
     3241 ctggcgacgg cgagacggat gctggccgag ctgacggagc aggcgcccgg tgggcagcgc
     3301 tgatatcatc gcgacaacga agcggcggag aatgacggct gtgcgatggg gcgcgatcac
     3361 cgcgtgttgg ctggtctggg ggctgttcta cgcgtcgcgg ctgcgcttgg tgatctccga
     3421 cgtctcgtgg gccgagacgt tgtcctacag catgccggac gccgtgctgt gggcggcgtt
     3481 gacgccgatc ccgctcgcgc tcgcccgccg cttcccgatc cgcgcggatc gctggtgggc
     3541 ccacatcccg ctgcacctcg cggccgcggg cgtcgtcgcc ggcgtgcacg gcctcctcga
     3601 cgccacgctg aacgtggtgt tcggctcgcc gctgtcgttc ggcgagatgc tgctgcacct
     3661 gctgcgcttc ggtgtgcaca cgaacgtgat gctgtacttc gccatcgtcg gcgtgtccca
     3721 gttcttcgcc cgcagccaac gcctgcgcga acgggagcgc caggcgtcgg agcttcgcgc
     3781 ccagctggcc caggcgcggc tcgacgcgtt gcagatgcag ctgcgcccgc acttcctgtt
     3841 caacgcgctc aacaccgtct ccggactggt cgagcaagac gtcgccgccg gccggcgagt
     3901 gctgcggcag ctcggcgacg tgctgcgcat gatcctgcaa cgccgcgacc gccaggaggt
     3961 ctccgtccgc gaggagctcg atctcgtccg ggcctatctc gcgatcgaat cggcgcggtt
     4021 cggcgagcgc ctgacggtgc aggtggacgt cgccgagaag acgctcggcc tggccgtccc
     4081 gtcgttcctg ctgcaaccgc tggtggagaa cgcggtgacc cacgcggtcg cgcgccggat
     4141 ccgcggaggg cggatcgacc tccgcgcgtg gtgcggcgac gggtcgcggc tcgtcgtcag
     4201 cgtcgaggac aacggggccg aggacgcgtc ggcaccggtg gagtccggcc agggcatcgg
     4261 tctgaccaac gcgcgagccc gactgcggca gctgtacggc gacgagcaag agctgcgcgt
     4321 gctgcgcaac gagcgcggcg gctggacggt gacggtctcg attccggcgc gctcgctggc
     4381 cgacgcgatg cggcgatcgg catgaagctg ctgcgcgcgc tggtcgtcga cgacgagccg
     4441 gcggcgcggc aggctctccg ctcgctgctc gagcacgtgg ccgacctccg cgtgatcggc
     4501 gagtgtgcga gcggcgccga ggcggtggag tcggttcatc gtctccgccc cgacctgctg
     4561 ttcctcgacg tcgagatgcc cgacggcaac ggtttcgacg tgctgcagca gctcgccccg
     4621 gggcacttgc ccgcggtggt cttcgtcacc gccttcgacc actacgccgc cgatgccttc
     4681 cgcgtcgacg cgatcgacta catgctcaag ccgttcgacg ccgagcgcct ggccgagacg
     4741 gtttcccgcg tgcgccgccg gctcgcccgc ggcgggccga cgccttcgga gtgggccgac
     4801 gcgttgcgcg cgctggccga ccgcggccgg tcgctcgagc gcgtggcggt gctcgacggc
     4861 gacgccagca tcgtgatcaa gacatcccag atccgctggc tcgagtcgca ggcgaactac
     4921 cttcgtcttc acgtcggcga ccggtcctac ctcgcccgcg gcacgatgcg ttcgatggag
     4981 cagcgcctcg acgcgacgcg tttcgtccgc atccaccgga cggcgatcgt caacctggac
     5041 cacgtgcgtc agctgcgccc gatcggccac ggcgacctgc agctcgttct ggccgacggc
     5101 acggagctga gcgtgagccg ccgctatcgg agcggcctgg agcggctggt ggagcgactg
     5161 gcctgatccg gggtagactt ctcgcgaggg gttcgaggag caccgatgaa agcgctggcg
     5221 ctcgtcctct gcgcggcgtg cgctgtgcca ccctccgtgg cgcaacaacc caagtacaaa
     5281 aatctccagg tcttcccggc ggacgtctcg gaggccgagc tcgacgaggc gatgctcgag
     5341 aacctgcgcg gactgggcct gcgccgactg gccggcgagg gctgcctcta ctgtcacgtc
     5401 ggcgatctcg aaacgccgcg cagcgagtgg aactacgcgt cggacgccaa gccggccaag
     5461 cagaaggcgc gcgtgatgat gcggatggtg cgcgcgatca acgaggagta cttgtccaag
     5521 ctgtcgccac gcgcggcgcc ggcgttccag gtgacttgca ccacgtgcca cgagggacgc
     5581 ctcgatcctc ggccgctgcc ggacgtcctg tgggcggtct acgagaccga cggcatcgac
     5641 ggcacgaccc ggcgctacca ggagctgcgc gacggctact tcggggccgc ggcctacgac
     5701 tttcgcgtcc acgtcttgtc cggcatcgcc acgcggatgg ccgaccgggg cgcgatcgac
     5761 gacgcgatcc gactggccga ggtcaacgtc gaggtctacc cccaagagcc ggccgccaag
     5821 cgggcctggg tcgcgctgac gctcgagcgc acgatcgacg ccgacggtgt cgatgcagcg
     5881 ctttcacagc tcgcgcggct ggagccgacg ctcgcggagg gcgtcgtgac gccgagcctg
     5941 ctcgactcgc tcgcttggcg actcaaccgg tccgagcgcg agacccaggg gcacgcgctg
     6001 atcgaggcca atttctcgaa atttcccggc gagtacaccc cgctcgagag cttggtcttc
     6061 gttcgctcga acagcggccg caaggacgaa gcgctcgcgc tgctcgaggg ttggctcgac
     6121 gagcacccgg accacgcgcg cgcccggcgg gtgtggatca acctgaccca gacgccgacg
     6181 gattaggccg aaccgcgagc gggtgctcga ctagagtccc gacaggtcgc cgaccacctc
     6241 gatcagcgcg ttccacaacc cgtcgtcgaa acagtcgcgc cgaccacagc tccgttcgcc
     6301 gagaatccgt cgcaccgcgt cggggccgac cggcccgccg cgaagctcgc ggatcagctc
     6361 ctcgtcgacg tcgaggattc tcatcgcgac cagccggtcc gcggtgaagt tccccagccc
     6421 cagctcggcc atctcgtcac ggaatctctc gtcgacctgg tggatccgaa gacgcagggc
     6481 ctgatcgacc gtcagaccgg tcagcccgag gtcggccatt tcgtcgatga acgcccggtc
     6541 gatccgatgg atgcggaacg cgatcaggtc gcccgagtcg aggccggcga accccagctc
     6601 cgccatctcg tcgatgaact ccgccgtgac gccgtggatg cggaacgcca tcgcgtcgtc
     6661 cgcgtcgagc tcgagcccca gcgcacgcat ctgctcgatg aacccgggtg agacgccgtg
     6721 gatgcggaag ccgatcagcg tgtccgggga gaaccgaccg agcgccagcc cgttcatctc
     6781 ctcgacgaaa cgtcgatcga ccccgtggat gcggaaggcg atcagctggt ccggttcgag
     6841 gcgaggcagc ccgacgtccc gcatctcctc gacgaaccgc tcggagacac cgtggatctt
     6901 gaacgcgacc agccggtcgt cgtcgaggtc gggatagccc aacgcggcca tccggtcgat
     6961 gaaatcctgc gtgaccccgt ggatctcgaa tgcggaccgt gcgtcatggg cggccgccgc
     7021 cggaaccagg agcgaaaaga ccagggacag aacggtcgag acgtgcgcgt atcgcaaggc
     7081 gtgaccctcc atgcccggtt agtactcggc ggaggccgtc gaggttacag ctcacggcca
     7141 cagccaaccg aaggtgccgc cggtcacggc cccgtagatc agcccgtcga acaggctctt
     7201 cagcgtcgtg ctccatttca tcccgaccca gatcgacccg gccggcgagg ccccggcgta
     7261 ggtcaggaac gcgaccgtgc cggcgacgcg gaagacggcc aggtagtcgg cgccggcatc
     7321 catcgtccgg ctcgcgacgt aggccgtcac gaccccgacg gccagcgtgt agacgaacca
     7381 ctgcacgagc tgcttgccca tcgtcggcgg cccggtgggc agcccgatca ccagcgcgtt
     7441 cggcccctcc ttcatcttcc cgacgaaggt ctcgtccttg aggtcggcca tcgaggagca
     7501 gtacggcatg cggtagttgc ccggcggcag tcctgtcagc gccgctcggg ccttgtcctc
     7561 tccgggaagc cggctccagt ccgtccagtg gtaacgaagc accatgtgga cgatcgagct
     7621 ggcgacgaac acgaacacgg cagacagcaa gatggggagc cacagaccgg tgatggaagc
     7681 catccgtttg tcctccactc agacccggcg gcgatcctac cgcatctcgg gtgattccgg
     7741 gtgcgggccg tccttgcgcc accccggtcc cggcctatat tcgaccccga tgagtctcac
     7801 cgtcaccgcc gccgtcggcc tgatcctggc gatcaaggtg ccgcagaccc gagccgagtt
     7861 cgtcgatgcc gtcgccgccg gccggggcgc gaccaaggtc gaaacggtcg tcgtcgagcg
     7921 cgacatcgac ggtgtctacc agaccctggc ggagcggtcc gcggcgtgcc tcgacgtgct
     7981 cgtcgagcgc tcgggcttcg tcgggacgca ctgggaggtc agctcgtccg actacaaccc
     8041 cacgctcgag cggatcgacg acggcaccgc ggccttcgcg ctgcaggtcg agcaccggcc
     8101 gcgtggcgtg ggtcacaaac cgccgcccgg cggtctctac gtgttcgccg cggagatcaa
     8161 ggcgctcgac gattcccgga ccgaagtcgt gctctaccgg ccgacgatgg ggttcaagaa
     8221 gatcgcggcg tccttccgcg cttggctcga cggcgacgac gccggctgcc cgaagctgcg
     8281 ctagccgcgg gcccccaacc gcttgacata taaccatacg cttggtatgg ttttccttat
     8341 gaagacgact ctcaatatcg acgattcggt gatggcccgc ctcaagcagg aagcggctcg
     8401 gtccggccgg accatgtccg agctggtcga aaccgccctc cggcagctgt tccggtctcg
     8461 tcgctcgcgg gaggagctac ccccgattcc caccttcgcc agcggcgggg cgctggtcga
     8521 cctggccgac cgcgatgcgc tctaccaggc gatggaagga cgctaggtcg ggtgttcgtc
     8581 gtggacacga acgtcctggt ctacgcggcg gaccgggact cgccccacca tgccgcgtgt
     8641 ctcgcgcgac tcgagtcctg gcgctcggcg aacgacgcct ggttcgttac gtggagcatc
     8701 ctctacgagt tcctgcgggt ggtcagccat ccgcgagtgc tgcgaaaacc gtggaccgtg
     8761 ccgcaggcct ggtcgttcgt cgaagcggtt ctcgcgagtc cgggactcga ggtcctcgcc
     8821 gaaaccgagc gacacgcaga cgtcgcgcga cggacgatcc gggaactgcc ccacctcgcg
     8881 ggcaacctgc tgcacgatgc ccacacggcg atcctgatgc gcgagcacgg ggtcagacga
     8941 atctacacgc gcgacaccga cttccaccgg ttcggtttcc tcgaaccgct cgacccgctc
     9001 gccgcgggtt gacggtggcg gccgcgtctc gtcgtcattc ccgccgttcg agccgaaacc
     9061 accgctgcat gtcatcgagc acgagtcgcc acccctcgac caccgtgtcg tgcatccaat
     9121 tccaatcccc gccggatcgg tacccgcgat gaggtgcccg accttcagcc gccgtggctc
     9181 cgcttcctcg accacaccga cccagacgtg ctgggtctgt tcctcgccgt agtcgtccca
     9241 ggcgacggac caggtcgtcc gcatcgacga acgcgcgcag cacccgctcc gcactcgcgg
     9301 gaatggagcc ggtcgtcgtg acggttcgca gctcgccgcc atcggtcgtc caactgccga
     9361 gcagcacgac cgagacgacg agcgcgacga atcgcggact tctcatcgac actggcctat
     9421 ccctccgcat cccgcgaact ctccctacat tacggccacc gcggggacag gcgtgagccg
     9481 accggtcccg gcaggtcctc gtagtgggag acgtcgttga acagcagcag ccgcgcctcg
     9541 ccgccgcgtt ggacgtcgag cacgttgagc gcgcacgggt cctgacccag ccggtcgcga
     9601 aagccgcgca gggcgaagcc gagcaagtgc ccgaccaaga gccggatcgt cgccttgtgc
     9661 gagacgacga gcaccgaggc gccgacgtgc cgctcggcga tcgccaacag gcgcggcagg
     9721 gcccggttca gcacctcgag tccggtctcg cctccgctcg gcgcgaaggt gaacgggtcg
     9781 gcctcccagg cgcggaactc ctccgtccac atccggcgta cctcgtcctt gcttttcccc
     9841 tcccactggc cgtgatcgat ctcgcgcaac tcgggaacga ccgtcgtctc gacatcgcga
     9901 ccgtcgagga tcagccgcgc cgtctctcgc gtacgcgtca tcgggctcgt gtaggcgaca
     9961 gcgagcgcct cgtgcgccag ccggcgcccg agcgccctgg cctgccgccg gccggtctcg
    10021 gcgagttcga cgtcggtcga gccggcgaag cggtcctcgg cggccgaggt cgtttcccca
    10081 tgacggacga ggaagatccg ggtgcgcatg cgggctttct cgacgggcat tctactcgcg
    10141 cgaactagag accgtccgga atctccggct cgacgagccg cgccagcatc gacagcgact
    10201 cctgccagcc gaggtagcag gcctcggccg gaatcacggc cgggacgccc tcctgcacga
    10261 tgcggagctc ggtcccgcag gagacctcgt cgagcatgat cgcgacctgc atctcgccgg
    10321 gcagattggg gtcgtcgaac cggtccgtgt agcggatgcg ccggtggggc tcgagctcga
    10381 cgtaccggcc gccgaacgag tggctcgtgc cggtgctgaa gttcgtgaac gacatgcggt
    10441 agccgccccc gacgcgcgca tcgatctcgt gcaccttgcc ggtgaagccg tgcgggggaa
    10501 tccacttgac taacgcctcg ggttcgagaa acgcccggta gacgcgctcg gccggggcgc
    10561 gaagcacgcg gtggagtcga atcgtgttcg tcgaactggc catggtcgga cctcctgaat
    10621 cctcgagttt gcggtcgttc agccggtagt cgttcgagcc gcgtgcggat cgacacccgg
    10681 cggcacgaaa cgggagaatc ttacgccgac ccgcgctact cgccgcgaat ccggcgctgg
    10741 aactcggtca gcagctcggc cgccgcgacg ttctccacca gcagatactg cgagacgagc
    10801 cagtcgaggc ggtggaccga ggcggcgtcg agcgggatgt agcccgcggt gagcacgacc
    10861 cacggcgcgt cgcggatctc ggaaatcgtc acgcttggtt cgacgtgctc gtggatccag
    10921 ccgagccgcc gggtgtagac ctcgatcccc gaacgacaat cgggtcgtat cgcgacccgc
    10981 ttcgagccgt cgtcgtccgc taggggctcg ccgtggggtc cgacggcgag cagcatctcc
    11041 gcgatggcgg caccctgcag ccgttcggcg acctcgtagt cgcgcagtcg aaacgcgatc
    11101 agtcccagct cgtcgagggt ttcggcaaaa cgccagtccc gcggcccgaa tccgctcgcg
    11161 gcgtacaggg cgcgacacat ctccgactcg gcgagtgcat agtc
//
LOCUS       DASPGN010000359         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_766685, whole genome shotgun sequence.
ACCESSION   DASPGN010000359 DASPGN010000000
VERSION     DASPGN010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766685"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VD788_10275"
     CDS             <1..792
                     /locus_tag="VD788_10275"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76694.1"
                     /translation="RPGDRPLRRIVVRTAEFSGEVGVGLYARAGVSGLDRLAARLMER
                     VPELAGVVRLHSPHGRRGGTRAEVMAGRGWIVEQVAGFRLELPATSFLQVSRAAAEAL
                     VERVAETAPALDGCHVLDLYGGVGLHALTAVRGGAASATVCEADVEAVRCGRETAARS
                     RSSGVRYVHADVGRFLARPAARAAAPHWVIANPPRSGLGTGVARAIRALGACRLSLVS
                     CDPATLARDLALLVEGGRYGIERIVPLDPFPQTAHVEVVATLGAR"
     gene            complement(815..1855)
                     /gene="holB"
                     /locus_tag="VD788_10280"
     CDS             complement(815..1855)
                     /gene="holB"
                     /locus_tag="VD788_10280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HXV76695.1"
                     /translation="MEPIVGQTAVVELLDGIRASGRVPHGLLLQGPDGVGKRAVARRF
                     AAGLLCDEPAAAPCGRCESCRLVDRDSHPDLAWVRRLPKVATGTETAADDESDLHPQI
                     KVDQIRELTRLLAMAARRGGRRVAILDPADRMNREAQNALLKTLEEPPGRAVLVLLAS
                     RPHLLLPTVRSRSFVVGVAALRVSELARLLVVERGMDRDEALTRAALAEGRPRRALDL
                     DLDAERARREGLLLCLERLGRGGAGIGELPDLATAVAGRTQDDLVDNLDMLEALLRDA
                     ARASLDAEDEALVHADEAPRLARIGERLGVLRAAELTRGIERLERDLRVNVNRTLVAE
                     AILAAVAGGPLP"
     gene            complement(1864..2547)
                     /gene="tmk"
                     /locus_tag="VD788_10285"
     CDS             complement(1864..2547)
                     /gene="tmk"
                     /locus_tag="VD788_10285"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXV76696.1"
                     /translation="MTARGTVGASGPWITFEGVEGTGKSTQLERLGRRLVARGRSVVV
                     TREPGGTGLGRRLRGVLLEPAEPPMDAFAELLLYTADRAQHLAEVVLPALDAGRVVLC
                     DRYLDATLAYQGHARGLGFARVLELHRTPPLDARPQRTLLFDMDPAVALGRARRRNAS
                     AASGEREDRFERERIEFHRRVREGYLMLAEREPTRIRLIDAAGEAQEVERAVCRQLAD
                     IFPELGSGE"
     gene            complement(2544..3482)
                     /locus_tag="VD788_10290"
     CDS             complement(2544..3482)
                     /locus_tag="VD788_10290"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HXV76697.1"
                     /translation="MHVWNGLDSMSRRMPAVVASIGKCDGVHLGHQEILRGVAREGRR
                     CELPALLISFDPHPATVVAPDRVPKLIQTRRQKLAALESTGLTDFLILEFDNELAALS
                     GERFFSDVLLPRLGFRSIHVGHGFRFGHGRSGDIELLRRIGERDGFAVHEVPPVVVDG
                     DVVSSSRIRDAVGRGEAERARRLLGRPFAVEGEVVPGRGRGRELYFPTANLRVDNELI
                     PRSGVYVTETRALAGRHASVTNVGVRPTFAESALTVETHLLDFDGDLYDQRIEVAFLA
                     RIRDEMRFDTATELADQIARDRAAAISYFQSPRIGS"
     gene            complement(3488..>4217)
                     /locus_tag="VD788_10295"
     CDS             complement(3488..>4217)
                     /locus_tag="VD788_10295"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV76698.1"
                     /translation="RYGRDLGLTDGLARLLAELDAVSELRWIRVHYLYPNTISRALIE
                     SMRDAERVVPYVDIPLQHAHPATLARMRRGGSGATHAALLGRFRAAMPDATLRSTFIV
                     GFPGETEDEFAALLEFVERERFHHLGVFRYSHEQGTPAHRLDDDVPAEVKRERSERLM
                     AAQQAIVFAHNRALVGRRVTVLVEGAHPETDHLLVGRTAAQAPEVDGQVLINDGDAVP
                     GRFVHVEITETAGYDVVGRVVGPA"
BASE COUNT          586 a         1544 c         1515 g          572 t
ORIGIN      
        1 cggccgggtg atcgcccgct gcggcggatc gtcgtccgca ccgccgagtt ctcgggcgag
       61 gtcggggtcg ggctctacgc gcgtgccggc gtctccggtc tcgatcggct cgccgcgcga
      121 ttgatggagc gggtgccgga gctcgccggg gtcgtccgtt tgcactcccc gcacggccgt
      181 cgcggcggca cgagagcgga ggtgatggcc ggacgagggt ggatcgtcga gcaggtcgcc
      241 ggcttccgcc tcgagctccc cgcgaccagc ttcctgcagg tcagccgggc ggcggccgag
      301 gcgctggtcg aacgggtcgc cgaaacggcg cccgcgctcg acggctgcca cgtgctcgac
      361 ctctacgggg gagtcggtct ccatgcgttg accgcggtgc gcggcggcgc tgcatccgcg
      421 acggtctgcg aggccgacgt cgaggcggtg cgctgcggtc gtgaaacggc ggcgcgtagc
      481 cgctcgagcg gggtgcgcta cgtacacgcc gacgtggggc ggttcctcgc acggccggcc
      541 gcccgggcgg cggcgccgca ctgggtgatc gccaacccgc cgcgcagcgg cctcggcacc
      601 ggcgtcgcgc gcgcgatccg cgcgctcggg gcttgccgcc tgtcgttggt ctcctgcgac
      661 ccggcgacgc tcgcccgcga cctggcgctg ctcgtcgaag gcggccgata cgggatcgaa
      721 cgcatcgtcc cgctcgaccc gttcccccaa accgcgcacg tcgaggtcgt ggccacgctc
      781 ggcgcccgct gatcgcgaaa ccgtcgctgt acgctcacgg cagggggccg ccggcgacgg
      841 cggccaggat cgcctcggcg accagcgtcc gattgacgtt gacgcgcagg tcgcgttcga
      901 gacgctcgat cccgcgcgtc agttcggccg ctcgcagcac gcccagacgc tcgccgattc
      961 gcgccaggcg cggggcctcg tcggcgtgca cgagcgcctc gtcctcggcg tcgagcgacg
     1021 cacgggccgc gtcgcgcaac agcgcctcga gcatgtcgag gttgtcgacg agatcgtcct
     1081 gcgtgcggcc cgcgaccgcc gtcgcgaggt ccggaagctc gccgatcccg gcgccgccgc
     1141 gccccagccg ttcgagacag agcagcagcc cttcgcgccg cgcgcgctcg gcgtcgagat
     1201 ctagatcgag cgcgcggcgc gggcggccct cggccagcgc ggcccgggtc aacgcctcgt
     1261 cgcggtccat cccgcgctcg acgaccagca gccgggccag ttcggagaca cgcaacgccg
     1321 cgacgccgac gacgaacgac cgcgagcgga cggtggggag cagcaggtgg gggcgcgacg
     1381 cgaggagcac gagcaccgcg cggcccggcg gttcctcgag cgtcttgagc agcgcgttct
     1441 gcgcctcgcg gttcatgcgg tcggccggat cgaggatcgc cacgcggcgg ccgccccgcc
     1501 gcgccgccat cgccagcagg cgcgtcagct cgcggatttg gtcgaccttg atctgcgggt
     1561 gcaggtccga ctcgtcgtcc gccgcggtct cggtccccgt ggcaaccttc ggcagccgcc
     1621 ggacccaggc cagatcgggg tggctgtcac ggtcgaccag ccggcacgat tcacagcggc
     1681 cgcacggcgc ggccgccggc tcgtcgcaca gcagtccggc ggcgaagcgc cgcgcgacgg
     1741 cgcgcttgcc gacgccgtcg gggccctgca gcagcaggcc gtgcgggacg cgcccgctcg
     1801 cgcggattcc gtcgagcagc tcgaccacgg cggtttgtcc gacgatcggt tccatcgcct
     1861 cgctcactcg ccgctaccga gctcggggaa gatgtcggcg agctgccggc acacggctcg
     1921 ctcgacttcc tgcgcttcgc ctgcggcgtc gatcaggcgg atccgagtcg gttcgcgttc
     1981 ggccagcatc agatagccct cgcgcacgcg gcggtggaac tcgatccgct cgcgctcgaa
     2041 gcggtcctcg cgttcgccgc tggcggcaga cgcgttgcgt cgacgcgccc gcccgagcgc
     2101 gacggcgggg tccatgtcga acagcagcgt ccgctgcggc cgcgcgtcga gcggcggagt
     2161 gcggtgcagc tcgagcacac gcgcgaaacc caagccgcgt gcgtgcccct ggtaggccag
     2221 cgtcgcgtcg aggtatcgat cgcagagcac gacccggccc gcatcgagcg ccggcagcac
     2281 cacctcggcg agatgttgcg cgcgatcggc ggtgtacaac agcagttcgg cgaaggcgtc
     2341 catcggaggt tccgccggct cgagcagtac gcctcgcagc cgccggccga gcccggtgcc
     2401 gccgggctcg cgggtgacga ccaccgaccg cccccgtgcg acgagccgcc gcccgagtcg
     2461 ctcgagctgc gtcgacttgc cggtgccctc gactccctcg aaggtgatcc acgggccaga
     2521 cgccccgacc gtcccgcgcg cggtcacgac ccgatccgcg gggactggaa gtacgagatc
     2581 gccgccgcgc ggtcgcgcgc gatctgatcg gcgagctcgg tcgcggtgtc gaatcgcatt
     2641 tcgtcgcgga tccgcgcgag gaacgcgacc tcgatccgtt ggtcgtacag atcgccgtcg
     2701 aagtccagca ggtgggtctc gacggtcaga gcggactcgg cgaaggtcgg ccgcacaccg
     2761 acgttggtca ccgacgcatg acgtccggcc agcgcccggg tctcggtgac gtaaaccccc
     2821 gagcgcggga tcagctcgtt gtcgacacgc aggttggcgg tgggaaagta cagctcacgc
     2881 ccgcgaccgc gccccggcac cacctcgccc tcgacggcga acggacgccc gagcaggcga
     2941 cgcgcgcgct cggcctctcc gcgaccgacc gcgtcgcgga tccgtgacga cgagacgacg
     3001 tcgccatcga cgacgacggg agggacctcg tggacggcga agccgtcgcg ctcgccgatc
     3061 cgccgcaaca gctcgatgtc acccgaccgg ccgtggccga agcggaatcc gtggccgacg
     3121 tggatcgagc ggaagccgag ccgcggcagc aggacgtcgc tgaagaaccg ttcgccgctg
     3181 agcgcggcca gctcgttgtc gaactcgagg atcagaaagt ccgtcagccc ggtcgactcg
     3241 agcgccgcca gcttctgtcg gcgggtctgg atcagcttcg gcacgcgatc cggcgcgacg
     3301 acggtcgccg gatgcgggtc gaagctgatc aacagcgccg gcagctcgca tcgccggccc
     3361 tcccgcgcga caccgcgcag gatctcctgg tggcccaggt ggactccgtc gcacttgccg
     3421 atgctggcga cgacggccgg catccgccgc gacatgctgt ccaacccgtt ccagacgtgc
     3481 atcgcgctca ggccgggccg acgactcgtc cgacgacgtc gtagcctgcc gtttcggtga
     3541 tttcgacgtg gacgaagcga ccgggcaccg cgtcaccgtc gttgatcagc acttggccgt
     3601 cgacctccgg tgcttgcgcg gcggtacggc cgacgagcag gtgatcggtc tccggatgtg
     3661 ctccctcgac cagcacggtc acgcgccggc cgaccagcgc gcggttgtgc gcgaacacga
     3721 tcgcctgttg cgccgccatc aagcgctcgc tccgctcccg cttgacctcg gccggcacgt
     3781 cgtcgtcgag acgatgggcc ggcgtacctt gctcgtggga atagcggaac acgccgaggt
     3841 gatggaaccg ctcccgctcg acgaactcga gcagcgcggc gaactcgtct tccgtctccc
     3901 ccggaaaccc gacgatgaag gtcgagcgca gcgtcgcgtc gggcatcgcc gcgcggaacc
     3961 ggccgagcag ggcggcgtgg gtggccccag aaccgccgcg gcgcatccgc gcgagcgtcg
     4021 ccgggtgcgc gtgctgcagc gggatgtcga cgtagggcac gacccgctcg gcgtcgcgca
     4081 tcgactcgat caaggcccgg gagatcgtgt tcggatagag gtagtgcacc cgaatccaac
     4141 gcagctcgct caccgcgtcg agctcggcga gcaaccgggc gagaccatcg gtcagcccga
     4201 gatcgcggcc ataacgg
//
LOCUS       DASPGN010000360         2916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_65081, whole genome shotgun sequence.
ACCESSION   DASPGN010000360 DASPGN010000000
VERSION     DASPGN010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65081"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2441
                     /locus_tag="VD788_10300"
     CDS             <1..2441
                     /locus_tag="VD788_10300"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXV76699.1"
                     /translation="AAERPSLTPGLLLALYGLSGLAAMAYQVAWSRALILAMGSSTYA
                     FSTIVACYIFGLALGSLLVSSAEARPAWRALVSRHTLSIAGYLQAAIGLSALLVVPLF
                     GRMPLWVAGLARGPEATFSDVLRAEALIVFGLLFVPTCCMGALMPLVCRLYGRDAGPG
                     RSMGDVYAANTIGTIVGAFSAGFVLIPLQSVGMQHTIHIASALSIAIGSLFLLGAERS
                     ARHGLAVVLLWIAGAATIVLGGTWAREVMVSGPFLARREIERSVLFYREGVDTTVAVT
                     SSDAVSRTLRVNGKPDASNSFMDMVTQLMSAHIPLLLRPDAEQVLVIGLGSGATGGAV
                     LSHPVERVDVAEISAAVVEAAAYFDAVNNGVLTDERVTIHREDGRNHLLLTDRRYDVI
                     VSEPSNPWISGVANLFTREFFELALSRLRPGGLHCQWIQGYSIRAEDVAAVLHTLGSV
                     FAHVQFWETGFGDYLVIGSEMPIELDLDRVYQTMALPEVALSLGRVLIQDPNQLAAHW
                     IAGRDELEQWIGGQRVLTDDRPYLEFAAPLFLLGSDEARIRQALLEFDEPPALAPSAP
                     PALAEEFRAALGQARSRRRAMHRLETAVMARDGGATLDGLLDVARWGATDARSMKRVD
                     QMLTAIEAESNPALAERVGATAERLRRDAPYLARAGADRTEPATMYWPFTRSFDYETD
                     AEAERLEAEILRAIEARDRERMMDQARRLLARAPQSWRALSTIGALLLDERGPEAARP
                     YLLKAWIRNPASRAVGHRLAHVYAGTGRPDLAIRFVERSIANGLSPELVAADPGLAAL
                     RDQPAFRELVDGTR"
     gene            2454..>2916
                     /locus_tag="VD788_10305"
     CDS             2454..>2916
                     /locus_tag="VD788_10305"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76700.1"
                     /translation="MKPWLVVKFGGTSVATAARWQVIADRACRALAEHRVWIVVSALA
                     GVSDLLERAITDAAAGRRSTAFGRARKLHDRLADELGLDDDARLPVTRLVERVDSLLR
                     GIRLTREAPPRLRAQILATGELCSTELGIHVLRARGLTAVRVDARDLLTSSE"
BASE COUNT          371 a         1012 c         1065 g          468 t
ORIGIN      
        1 ccgctgccga gcggccgagc ctgacccccg gtctgctgct cgcgctctac ggcctctccg
       61 gactcgcggc gatggcgtac caggtcgcct ggtcgcgcgc gctgatcctg gcgatgggct
      121 cctcgaccta cgccttcagc acgatcgtcg cctgttacat cttcggcctg gcgctgggca
      181 gcctgctcgt ctcgtcggcc gaggcgcgcc cggcctggcg cgcgctcgtc tcgcggcaca
      241 cgctgtcgat cgcgggatac ctgcaggcgg cgatcggact gagcgccttg ctcgtcgtcc
      301 cgctgttcgg tcggatgccg ctgtgggtcg ccggcttggc ccgcggaccc gaggcgacct
      361 tctcggacgt gctgcgcgcc gaggcgctga tcgtcttcgg cctgctgttc gttccgacct
      421 gttgcatggg tgcgctgatg ccgttggtct gccggctcta cgggcgcgac gcgggaccgg
      481 gccgcagcat gggcgacgtc tacgcggcca acacgatcgg gacgatcgtc ggcgcgtttt
      541 ccgccgggtt cgtcctgatc ccgctccagt cggtcggcat gcagcatacg atccacatcg
      601 cgtcggcgct cagcatcgcg atcggttcgc tcttcctgct cggcgccgag cgcagcgcgc
      661 gccacgggct cgccgtcgtc ctgctctgga tcgccggcgc cgcgacgatc gtgctcggcg
      721 gcacgtgggc tcgcgaagtg atggtgtcgg gaccgtttct cgcgcggcgc gagatcgagc
      781 gctcggtgct gttctatcgc gaaggggtcg atacgaccgt ggcggtcacg tcgagtgacg
      841 ccgtgtcacg cacgctgcgc gtcaacggga agccggacgc gtcgaactcg ttcatggaca
      901 tggtgacgca gctgatgtcg gcccacatcc cgctgctgct gcgaccggac gccgaacagg
      961 tgctggtgat cgggctgggt tccggtgcga ccggcggcgc cgtgctgagc catccggtcg
     1021 agcgggtcga cgtggccgag atctccgcgg ccgtcgtcga ggcggcggcc tacttcgacg
     1081 cggtcaacaa cggcgtgctg accgacgagc gcgtgacgat ccatcgcgag gacggccgga
     1141 accacctgct gttgaccgac cggcgctacg acgtcatcgt gtcggagccg agcaacccgt
     1201 ggatcagcgg cgtggccaac ctgttcacgc gcgagttctt cgagctggca ctgagccgct
     1261 tgcgccccgg cggcttgcac tgccagtgga tccaaggcta ctcgatccgg gccgaggacg
     1321 tggccgcggt gctccacacg ctcggcagcg tcttcgcgca cgtccagttc tgggagaccg
     1381 gcttcggcga ctacctggtg atcggctcgg agatgccgat cgagctcgac ctcgatcgcg
     1441 tctatcaaac gatggcgctg cccgaggtcg cactgtccct cggtcgagtg ctgatccagg
     1501 acccgaacca gctcgccgct cactggatcg cgggacgcga cgagctcgag cagtggatcg
     1561 gcgggcaacg tgtgctgacc gacgatcggc cgtatctcga gttcgccgct ccgctgttcc
     1621 tgctcggttc ggacgaggcg cggatccgcc aggcgttgct cgagttcgac gagccgccgg
     1681 cgctcgcgcc gtcggctccg cccgcgctgg ccgaggagtt ccgtgccgcg ctggggcaag
     1741 cacgcagccg gcggcgggcg atgcatcggc tcgagacggc ggtgatggcg cgtgacggcg
     1801 gcgccacgct cgatggcctg ctcgacgtgg cgcgctgggg cgcgaccgac gcgcgatcga
     1861 tgaagcgcgt cgatcagatg ctgacggcga tcgaggccga atcgaaccca gcgctcgccg
     1921 agcgggtcgg ggcgaccgcc gagcgcctgc gccgcgacgc gccctacctc gcgcgggccg
     1981 gggcggatcg gaccgagccg gcgacgatgt actggccgtt cacgcggagc ttcgactacg
     2041 agaccgatgc cgaggccgag cggctcgagg cggagatcct ccgggcgatc gaggcccgcg
     2101 atcgcgagcg aatgatggac caggcgagac ggctgctcgc gcgagccccg caatcgtggc
     2161 gcgcgttgtc gacgatcggc gcgctcttgc tcgacgagcg cggtcccgag gctgcgcggc
     2221 cgtacttgct caaagcgtgg atccgcaacc ccgcgtcgcg ggcggtcggg caccggctcg
     2281 cacacgtcta cgccggcacg ggacggcccg acctcgccat ccgtttcgtc gagcggtcga
     2341 tcgccaacgg cctgtcgccc gagctcgtcg cggcggaccc gggcctggcg gcgctgcgcg
     2401 accagcccgc cttccgtgag ctcgtcgacg ggacgcgtta gcggacgatg gcgatgaaac
     2461 cgtggctcgt ggtcaagttc ggcggcacct cggtggcgac ggcggcgcgc tggcaggtga
     2521 tcgccgaccg cgcctgccgg gcgctggccg agcaccgcgt ctggatcgtc gtctcggcgc
     2581 tcgccggtgt ctccgacctg ctcgagcgcg cgatcacgga cgcggcggcc gggcggcgct
     2641 cgacggcctt cgggcgcgcg cgaaaactgc atgaccggct ggccgacgag ctggggttgg
     2701 acgacgacgc gcggctgccg gtgacgcggc tcgtcgagcg tgtggactcg ctgctgcgcg
     2761 ggatccgcct gacgcgagaa gcgccgccgc ggctgcgggc gcagatcttg gccaccggcg
     2821 aactgtgctc gaccgagctc ggcatccacg tgctgcgtgc tcgcgggctg accgcggtgc
     2881 gggtcgacgc tcgcgacctg ttgaccagca gcgagc
//
LOCUS       DASPGN010000361         4686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1587915, whole genome shotgun sequence.
ACCESSION   DASPGN010000361 DASPGN010000000
VERSION     DASPGN010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587915"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VD788_10310"
     CDS             complement(<1..243)
                     /locus_tag="VD788_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76701.1"
                     /translation="MTAGFGASTFAFVWQATALEALRRMRGLGLDGFDVILAPGHCWP
                     AELDGRARRQVARALDADGIRVDSVNFPALDLNLASP"
     gene            complement(240..1076)
                     /locus_tag="VD788_10315"
     CDS             complement(240..1076)
                     /locus_tag="VD788_10315"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76702.1"
                     /translation="MAGTITGSTRVLGIIGDPIAQARSPEVWTGLFRLNGVDAVCVPL
                     QVSPAGLGAFLDGARALRNVVGLIVTIPHKAASLPHVASLTERADRVGAVNVIRLTEG
                     GWHGDTVDGIGFVAGLRRRGHEPAGRRSLVVGVGGAGAAIAFALAEAGAAEVAVFDLD
                     TERATDVSARLAATGIASRVAPAAAAGFDLVVNASPLGMAPDDPLPIALDGVDPGCIV
                     GDVVVHPEPTTLLRAAADRGCFTQPGVDMMDGQIAPMAMFLGLGAGRWDADAVAEVVG
                     ER"
     gene            complement(1080..1766)
                     /locus_tag="VD788_10320"
     CDS             complement(1080..1766)
                     /locus_tag="VD788_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76703.1"
                     /translation="MPLRGTAFLAIWHDIEPHGELEYNAWHTREHMPERVGIPGFEAG
                     RRFVDWSRDRHRYFTLYETRTIAALGSDAYRARLDAPSAWTHRVQPSFTNFVRAACRT
                     VVSEGRGTGGALATIRIDFGGAVDRSAFETACGVLAADALSRHGITGVHVGVADPAVT
                     DTPTTESALRELTGEDVFDAAVLVEGIGRRELAEALPAVEQSLAPAIGGAATAAAVYD
                     LAYSLTAEEV"
     gene            1845..3002
                     /locus_tag="VD788_10325"
     CDS             1845..3002
                     /locus_tag="VD788_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXV76704.1"
                     /translation="MQITSVTTTAVRTSMREPLTWPGGVRQSASGLVVEVHTDEGISG
                     IGEAPGPTLPTIQTIIEHELAQFLVGQDPLRTEWLVNRMEEFSRNWRGIAAYAISGLE
                     IALLDLKGKALGAPVAELLGGFCSDRVPVVGYLFIDEPEANARKAKAFVDAGYTELKL
                     KVGRDFAQDHDAIAAIRDAVGPDVKLRIDANMIWGVPAAIKWIRGLERFDLQYVEQPV
                     PDFDVAGLAQVRRSVGVPIAADEACTDVRSALELVKQDACDVFVVYPSEAGGLTRARQ
                     IAAIAEAAGKWCAIGSWAELGPATIANAHLVAATTTFGFASDTHYPLQLEDVLRAPLD
                     MSDGLLAVPRSPGLGVELDREATTRLANHQVRESVFYDDIHGEAPRVGQIV"
     gene            3027..4652
                     /locus_tag="VD788_10330"
     CDS             3027..4652
                     /locus_tag="VD788_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV76705.1"
                     /translation="MSDTPTTDTKGGGGELSRRDLLVKAGVVGAGAVAAGSVAGKATA
                     APKRVGAAPRKGGKLTFALEQDPVHIAPFGAILTSNHWGKEPMYESLIEWDAKLNQRP
                     ALAQSWKVVDPRTVDFTLRRGVRFHNGKEVTAADVKYSVEGWLNPPLPGAATTVNQVP
                     SIESATVRSKYVVRIRLKKPDARLFGFLAWTRYSAIVPEGMYQQVNAGREGIGTGPFR
                     LVSFTPGQGTEYQAYANYRKAGLPYLNQLSLPTMPDEQARIAALRAGAIDGATLSADG
                     ARTFQGNSNFTVLRGLTAAFRELQMTQKTGEDKPWHDVRVRQAVNFAINRQEILNRVY
                     NGFGEYSGHVPPGYGPWPLTRAQLRNQYLKFDLAKARTLMRQAGFANGFEVTMSTFST
                     PLDFGQVAAVIKSQLDRIGIEVNIKAEEPGTFAANNGAGRFEWDLTARGMRGDVDGYT
                     SEFNPANAIYKVWYPAYRNVRMWRLVGNGFITLDEKKRLAMYKELQRILLTEQLQVPL
                     VAVSKFQVVRRRVKNMYVAFSDFNTGLKTAWVQ"
BASE COUNT          733 a         1648 c         1673 g          632 t
ORIGIN      
        1 cgggctcgcg agattcaggt cgagcgccgg gaagttgacg gagtcgactc ggatgccgtc
       61 cgcgtcgagg gcgcgtgcga cctgacgccg ggcgcgtccg tcgagctccg cgggccagca
      121 gtgaccgggc gcgaggatga cgtcgaaccc gtcgagcccg agcccgcgca tccgtcgcag
      181 cgcctcgagc gcggtcgcct gccacacgaa cgcgaaggtg ctcgccccga agccggccgt
      241 caccgctcgc cgaccacttc ggcgaccgcg tccgcgtccc agcggcccgc gccgagcccg
      301 aggaacatcg ccatcggcgc gatctgcccg tccatcatgt cgacgcccgg ctgcgtgaag
      361 cagccgcggt ctgctgcggc tctcaggagc gtcgtcggct cggggtggac gacgacgtcg
      421 ccgacgatgc agccggggtc gacgccgtcg agcgcgatcg gcagcggatc gtcgggcgcc
      481 atcccgagcg ggctggcgtt gacgacgagg tcgaatccgg ccgccgcggc gggcgcgacc
      541 cgggacgcga tgcccgtcgc ggcgagccgt gccgacacgt cggtcgcacg ctcggtgtcg
      601 aggtcgaaca ccgcgacctc cgcggccccg gcctcggcga gagcgaaggc gatcgcggcc
      661 cccgccccgc ccacgccgac gacgagggag cgtcggcccg caggctcatg cccgcgcctg
      721 cgcaggccgg cgacgaagcc gatcccgtcg accgtgtcgc cgtgccagcc gccttccgtg
      781 agccggatca cgttcaccgc gccgacccgg tcggcgcgct ccgtcagtga ggccacgtgt
      841 gggagcgacg ccgccttgtg cgggatcgtc acgatcagcc cgacgacgtt ccggagcgct
      901 ctcgcgccgt cgaggaacgc gccgagccct gccggcgaca cctgcagcgg gacgcagacg
      961 gcgtcgaccc cgttgaggcg gaacagcccc gtccagacct cgggcgagcg tgcctgggcg
     1021 atcgggtcgc cgatgatgcc gaggacgcgc gtcgagccgg tgatcgtgcc cgccacggct
     1081 cagacctcct ccgccgtgag cgagtaggca aggtcgtaca ccgccgcggc ggtcgcggcg
     1141 ccgccgatcg ccggcgccag cgactgctcg acggcgggga gagcctcggc gagctcgcgc
     1201 cggccgatcc cctcgaccag gaccgctgcg tcgaacacgt cctcgcccgt gagctcccgg
     1261 agcgcggact cggtggtcgg cgtgtcggtg accgccgggt cggcgacacc gacgtggacg
     1321 cccgtgatgc cgtgccgcga gagcgcgtcg gcggcgagga cgccgcacgc cgtctcgaac
     1381 gcgctccggt cgacggcgcc gccgaagtcg atccggatcg tcgcgagcgc cccgccggtg
     1441 ccccgtccct cgctgacgac cgtccggcag gccgcgcgca cgaagttcgt gaacgacggc
     1501 tgcacccggt gcgtccatgc ggacggcgcg tcgaggcggg cacggtaggc atccgagccc
     1561 agcgcggcga tcgtccgcgt ctcgtagagc gtgaagtacc ggtgccggtc gcggctccag
     1621 tcgacgaagc ggcgtcccgc ctcgaacccc gggatcccga cgcgctccgg catgtgctcg
     1681 cgcgtgtgcc aggcgttgta ctccagctcg ccgtgcggct cgatgtcgtg ccagatggcg
     1741 agaaacgccg ttcccctcag cggcatggga cagttcgtgg caattgtcga caatcgacgg
     1801 atggttgaca atcgcgcatc gcgcgcctag tctagccagc ctcagtgcag atcaccagtg
     1861 tcacgacgac tgcggtccgg acgtcgatgc gtgagccgct gacgtggccg ggcggcgtgc
     1921 gccagtccgc ctccggcctc gtcgtcgagg tccacaccga cgaggggatc tcggggatcg
     1981 gcgaggcgcc gggaccgacg cttccgacga tccagacgat catcgagcac gagctcgcgc
     2041 agttcctcgt cggccaggac ccgctccgca cggagtggct cgtcaaccgg atggaggagt
     2101 tctcccgcaa ctggcgcggg atcgccgcgt acgcgatctc cgggctcgag atcgcgctcc
     2161 tcgacctgaa gggcaaggcg ctcggagcgc ccgtcgccga gctgctcggc gggttctgct
     2221 cggaccgcgt cccggtcgtg ggctatctct tcatcgacga gccggaggcg aacgcccgca
     2281 aggcgaaggc gttcgtcgac gcgggctaca cggagctgaa gctcaaggtc ggccgcgact
     2341 tcgcgcagga tcacgacgcg atcgcggcga tccgcgacgc ggtcgggccg gacgtcaagc
     2401 tcaggatcga cgcgaacatg atctggggcg tcccggccgc gatcaagtgg atccgcggcc
     2461 tcgagcgctt cgacctccag tacgtcgagc agcccgtgcc ggacttcgac gtggcggggc
     2521 tggcgcaggt gcggcgctcg gtcggcgtgc cgatcgccgc cgacgaggcg tgcacggacg
     2581 tccgttccgc gctcgagctc gtcaagcagg acgcctgcga cgtcttcgtc gtctatccct
     2641 cggaggccgg cggcctcacg cgggcgcggc agatcgccgc gatcgcggag gctgccggca
     2701 aatggtgcgc gatcgggagc tgggcggagc tcgggccggc gacgatcgcc aacgcccacc
     2761 tcgtcgccgc gacgacgacg ttcgggttcg cgagcgacac ccactacccg ctccagctcg
     2821 aggacgttct ccgggcaccg ctcgacatgt cggacggcct gctcgccgtg ccgcggtcgc
     2881 ccggtctcgg cgtcgagctc gaccgcgagg cgacgacgcg gctcgcaaac catcaggtgc
     2941 gcgaatccgt cttttacgac gacatccatg gagaggcacc gagggtcggt cagatcgtct
     3001 gacgaccgag ggaaggaggg acagacgtgt cggacactcc gacaacggac accaagggcg
     3061 gtggcggcga gctcagtcgt cgcgacctcc tcgtcaaggc cggcgtcgtc ggtgcaggtg
     3121 ccgtggctgc cggctcggtg gccggcaagg cgacggctgc accgaagcgc gtcggggcgg
     3181 cgccgaggaa gggcggcaag ctcacgttcg ccctggagca ggacccggtg cacatcgcgc
     3241 cgttcggggc gatcctcacc tcgaaccact ggggcaagga gccgatgtac gagtccctca
     3301 tcgaatggga cgcgaagctc aaccagcgcc cggcgctcgc gcagagctgg aaggtcgtcg
     3361 acccccggac ggtcgacttc acgcttcgcc gcggcgtgcg cttccacaac ggcaaggagg
     3421 tcaccgcggc cgacgtcaag tactcggtcg agggctggct caacccgccg ctgccgggcg
     3481 cggcgacgac ggtgaaccag gtgccgtcga tcgagtcggc cacggtgcgg agcaagtacg
     3541 tcgttcggat ccgcctcaag aagcccgacg cgcggctgtt cggcttcctc gcctggacgc
     3601 gctactcggc gatcgtcccc gagggcatgt accagcaggt caacgccgga cgggagggga
     3661 tcggcacggg gccgttccgc ctcgtgagct tcacgccggg tcagggcacc gagtaccagg
     3721 cgtacgcgaa ctaccgcaag gcagggctgc cgtacctcaa ccagctctcg ctgccgacga
     3781 tgccggacga gcaggcacgg atcgcggcac tgcgggcagg ggcgatcgac ggggcgacgc
     3841 tctccgccga cggggcgcgg acgttccagg gcaacagcaa cttcaccgtc ctccgcgggc
     3901 tcaccgcggc gttccgcgag ctgcagatga cgcagaagac aggcgaggac aagccgtggc
     3961 acgacgtgcg cgtccggcag gcggtcaact tcgcgatcaa ccgccaggag atcctcaacc
     4021 gcgtctacaa cgggttcggc gagtactcgg ggcacgtgcc gccggggtac gggccgtggc
     4081 ccctgacacg ggcgcagctc cgcaaccagt acctcaagtt cgatctcgcg aaggcccgca
     4141 cgctcatgcg gcaggcaggc ttcgcgaacg ggttcgaggt gacgatgtcg acgttctcga
     4201 cgccgctcga cttcggccag gtggcggcgg tgatcaagtc gcagctcgac cggatcggga
     4261 tcgaggtcaa catcaaggcc gaggagcccg gcacgttcgc cgcgaacaac ggcgccggcc
     4321 ggttcgagtg ggacctgacc gcgcgcggca tgcgcggcga cgtcgacggc tacacgtcgg
     4381 agttcaaccc ggcgaacgcc atctacaagg tctggtaccc cgcctaccgg aacgtcagga
     4441 tgtggcggct cgtcggcaac gggttcatca cgctcgacga gaagaagcgg ctcgcgatgt
     4501 acaaggaact gcagcggatc ctgctcaccg agcagctgca ggtgccgctc gtcgcggtct
     4561 ccaagttcca ggtcgtgcgc aggcgcgtga agaacatgta cgtcgcgttc agcgacttca
     4621 acaccggtct caagaccgcc tgggtgcagt aagaggtggg ggaacccaag gttacccccc
     4681 ccccag
//
LOCUS       DASPGN010000362         5492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_651052, whole genome shotgun sequence.
ACCESSION   DASPGN010000362 DASPGN010000000
VERSION     DASPGN010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5492
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651052"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VD788_10335"
     CDS             complement(<1..449)
                     /locus_tag="VD788_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76706.1"
                     /translation="MELGPGEVADRAQRVGVRIAERASPPVENVRQQRLRLVVATRGV
                     EGQCESVLRRERVRVIGSREAQPRRQRLAVQPFRFRPVAAVVVEPGQPVARGERQRSV
                     GVVFALERVDRARELPLRRLVQAERSQRLAEIAAQPGLDLRSVGELRV"
     gene            484..1575
                     /locus_tag="VD788_10340"
     CDS             484..1575
                     /locus_tag="VD788_10340"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76707.1"
                     /translation="MLDGHRRTLGADAPDTLRAGIELAAAVQAQGRFDEAEQLHREAL
                     AGLTRSLGADHPETLIAASDLGLLLAESGRYDDAERAFLAAIDGARRALGESHPRTLV
                     MMMDLANGRDGQGRYADAERLHREVLRTAGDTLGADHPITLAATNGLATNLHRQGRYP
                     EAEPLYRSAYEGRRAKLGVEHQDTQASINNLVQLLSALGRYDEATSLAAELVETRRRL
                     LGPDHPSTLVSMYGLAGLRSGQGRADEALAVYRETTERMERVLGPDHPWTLAGRNGYA
                     WLLLTADPTDLRDPQLALGLAEETCARSGRKNPYHLDTLALAWFRVGQTDKAIEAVRE
                     AIAVAPPENARLGDELAAHLAGYLAGEGP"
     gene            complement(1589..2311)
                     /locus_tag="VD788_10345"
     CDS             complement(1589..2311)
                     /locus_tag="VD788_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV76708.1"
                     /translation="MEQDPAFHTTRWSRVLAARGVDEPEARRALAELCGAYWYPLYSF
                     LRRRGHPAEEAADLTQGYFAELLEKRFLDDVHPERGKLRSFLLVSLKHYVSHERERQR
                     SQKRSVRHRAISIDAAEAEQRYRFEPADPVTPETIYERRWALTVIERARARLADEMTA
                     AGKREQYRALAELISFGRTQRSEREIGESIGLSEGAVGMALLRLRRRLGRLLREEIAH
                     TVHDRSHVDEELHHLLTVLQEL"
     gene            2593..4278
                     /locus_tag="VD788_10350"
                     /pseudo
     CDS             2593..4278
                     /locus_tag="VD788_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
     gene            4534..4686
                     /locus_tag="VD788_10355"
                     /pseudo
     CDS             4534..4686
                     /locus_tag="VD788_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4957..>5492
                     /locus_tag="VD788_10360"
     CDS             4957..>5492
                     /locus_tag="VD788_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HXV76709.1"
                     /translation="MGGLPATVDAWLEDGDGRAVARAIPSNVAPGERGFRFRPRCDRQ
                     YRLVLQTGDGWNGSATFLTTAAVVDGTSPSPWLPIPAPSDPPPPIPDADADGVWDEVD
                     TCPQVADPTNVDADLDGFGDACDVCPATANPDQLSFEFEPIFATSKTQLSWTTPVDYD
                     YAVGPLDDVANLTTWLTG"
BASE COUNT          904 a         1955 c         1872 g          761 t
ORIGIN      
        1 acgcggagtt caccgaccga ccggagatcg aggccaggct gcgcagcgat ctcggcgagg
       61 cgctgcgagc gctcggcctg tacgaggcgg cggagcggca gctcgcgagc gcggtcgaca
      121 cgctcgagcg cgaagacgac gccgacgctc cgttggcgct cgcctcgcgc taccggctgg
      181 ccaggctcca cgacaaccgc ggccacgggg cggaagcgga acggctgcac cgcgagacgc
      241 tggcggcgcg gctgcgcctc tcgggacccg atcacccgga cacgctcgcg tcgaagaacg
      301 ctctcgcact gaccctcgac gccgcgggtc gccacgacga ggcggagacg ctgttggcgg
      361 acgttctcga ccggcggcga cgcacgctcg gcgatgcgca ccccgacacg ctgcgcacga
      421 tcggcgacct cgcccggacc gagctccacc gcgggcgcgg cgcggaggcc gagcgttcgc
      481 gaggtgctcg acggccaccg ccgcacgctg ggagccgacg ctcccgacac gctccgcgcc
      541 gggatcgagc tggccgcggc ggtgcaggcg caaggccgat tcgacgaggc cgagcagctc
      601 catcgcgagg cgctcgccgg gctgacgcgc tcgctcggcg ccgaccaccc ggaaacgctg
      661 atcgccgcga gtgacctcgg tcttctgctc gccgagtcgg gccgctacga cgacgcggag
      721 cgggcgttcc tcgcggcgat cgacggggcc cgccgcgcgc tcggcgagag tcatccgagg
      781 acgctcgtga tgatgatgga cctggccaac ggccgtgacg gacagggccg ctacgccgac
      841 gccgagcggc tgcaccgcga ggtgctgcga accgcgggcg acacgctcgg cgcggatcac
      901 ccgatcacgc tcgccgcgac caacgggctc gccacgaacc tccaccggca agggcgctac
      961 cccgaggcgg aaccgctgta ccggtcggcg tacgagggcc ggcgggcgaa gctcggcgtc
     1021 gagcaccagg acacccaggc ctcgatcaac aacctcgtcc agctcctgtc ggccctcggt
     1081 cgatacgacg aggcgacgtc gctcgccgcg gagctggtcg agacgagacg ccggctgctg
     1141 gggccggacc atccgtcgac cctggtctcg atgtacggcc tggccgggct gcgctccggg
     1201 cagggccgcg ccgacgaggc cctcgcggtc taccgcgaga cgacggagcg gatggagcgc
     1261 gtactcggcc ccgaccaccc ctggacgctc gccggccgga acggctacgc ctggctgctg
     1321 ctcaccgccg atcccaccga cctgcgcgac ccgcagctcg cgctcgggct ggccgaggag
     1381 acctgcgcgc ggagcggacg caagaacccg taccacctcg acacgctcgc gctcgcctgg
     1441 tttcgcgtcg gccagacgga caaggcgatc gaagcggtgc gcgaggcgat cgcggtggcg
     1501 ccgccggaga acgcgcgtct cggcgacgag ctggcggcgc atctcgccgg ctacctggcg
     1561 ggcgagggac cctgaccgcg gggcggagct acagctcctg cagcacggtc agtaggtggt
     1621 gcagctcttc gtcgacgtgc gatcggtcgt gcaccgtgtg cgcgatctcc tcgcgcagta
     1681 gccggccgag tcgccggcgc agacgcagca gcgccatccc gaccgcaccc tcgctgagcc
     1741 cgatcgactc gccgatctcc cgttcgctgc gttgggtgcg gccgaacgag atcagctcgg
     1801 cgagcgcccg gtactgttcg cgcttgccgg cggccgtcat ctcatcggcg agacgcgcgc
     1861 gcgctcgttc gatgaccgtc aacgcccatc gacgctcgta gatcgtctcg ggcgtgacgg
     1921 ggtcggccgg ctcgaagcga tatcgctgtt cggcctccgc cgcatcgatc gagatcgctc
     1981 gatggcgaac cgagcgcttc tgcgaacgtt gccgctcgcg ctcgtgcgag acgtagtgct
     2041 tgagcgacac caagaggaac gaccgcagct tgccccgctc ggggtggacg tcgtcgagga
     2101 agcgcttctc gagcagctcg gcgaagtagc cttgggtcag gtccgccgcc tcctcggccg
     2161 ggtgcccgcg ccggcgcagg aacgagtaga gcggatacca gtaggcgccg cacagctcgg
     2221 cgagcgcccg ccgcgcctcg ggttcgtcga cgccgcgtgc cgccaggacg cggctccatc
     2281 gggtcgtgtg gaacgccggg tcctgctcca ccgccgcccc cttcgcggcc cattgtagga
     2341 cccctgggga aaaggacgtt agatcccgga cccgggccgt ctcttccgtt agaggagggc
     2401 gaagccatgg ctagcatgag gtcgttcggg aagctgacgg cgctggcaat cgtcgcagcg
     2461 atcgcggtcg ggtggagccc ggtcagccgg ggatacacgt cgccgcagca gcaggccctc
     2521 gtcgagctac gggccgcatc ggcgggcccc gtgcgcgtcg cctaccagca cggcttcccg
     2581 atcgtcgttt cgatgcgcgt gccggctgac ggcgccgacc cgctcgagca ggcatggagc
     2641 ttcctcgcga cctacggccc cctgtacctg ctcgatcagg aaggtgtgga gctggtgcac
     2701 tcgcgcaccg acgggaacga cggcgagctg gcggtccggt tcggccagcg gtaccggggc
     2761 cttacggtgt tcggcgccga actggtcgtg cacctcgcga tcgatccgac cgggccgtcg
     2821 ctggtcaccg agacgcaggg caatctgctg gtgccggcgc tcgccgatcg caaccctccg
     2881 caggcgatcg aaatcgacga gacgccggcg ttcaccgccg acgccgccga gacgatcgcc
     2941 cgcgatcggc tcggtctggg ggcggcgccg gtcgagggcg tcacgacgtt gatgctgtac
     3001 tccgactgga tcttgtccga tgcggctcag atcacgcagg cgtcgttgcc cggcgcggtg
     3061 ctggtctacc gcgtcagcct ggccgcgccg accggtccgt tgcaagtgct ggtcgatgcg
     3121 aacagcggcg agatcgtgtt cgagcacggc ctgagcgagc aagcccacca gatggaaatg
     3181 cgcgactcga tgggcaccgt gttcggcgac tcgtgtctgt ggctggtctg gccccaactc
     3241 ttcccactcg ccggcaacca caacggtttg accgccgcgt accagggcca tccggattcg
     3301 aacgccctgt ggtgggcgtc gcttgcgacc tacaacaagt accagagtct cggtcgggac
     3361 tcgtacgacg acgacgggag ctggatcctg atcacgctca acgcgttgtt cgagcccgac
     3421 gaccccaaca aaccgcccga gaccaacgcc agggcgggaa actgcggtct gcagttcagc
     3481 ccgggctacg gcagcaccga cgtcttcgcc cacgagttct cccatctcgt gatcaacgag
     3541 acctcggacc tggtctacga ggggcattcc ggcgcgctcg acgagtcgtt cgccgacgtg
     3601 atgggtgcga tggtcgacga cgacgactgg ctgatcggcg agggcacgac cggcagcgtc
     3661 ttcggtgccg gggcgctacg cagcctgcag aatccgccgg cctacaactg cgccggcggc
     3721 agctttccgt gccccgatca cttgtcccag tggtggtcgg acccggacga caaggacaac
     3781 ggcggcgtgc acaccaacag caacatcatg aacaaggcgt tctacctgat gaccgtcgag
     3841 ggcgactacg gctacgtgct gatcagcggg atggggcgga ccaagacgac ccggctggcc
     3901 tacgaggtct tcacccgtct tccgaagaac gcgaacttca gccaggcgtc gtggggcctg
     3961 atcaacaagg cgtggcagtg gatcaacaac aagcacggct tcacctattc cgatgcctgc
     4021 ggggtgcaca acgcgctcgc gatcaccgga atccgctggc cggatttcga ctgcgacggg
     4081 ggcgaggacg acgcgaccga caccgacggc gattgggtgt tcgacgacgg agacggatcg
     4141 gacaccgtcg gagacaatcc gtgctactcg ttcaagaact ccaactgcga cgacaactgc
     4201 atcaaccagc ccaatccgac tcaggtcgac ttcgacgacg acggcatggg tgacgcgtgc
     4261 gacttcgacg acgacgacga cggcgtgccg gactggtacg atccgtgtat cggcgtgccg
     4321 aacgacccct cggactggga cgacgacgac atcccgagct gtctcgatcc cgacgacgac
     4381 ggcgacggcg tgccggacga cggggacggc gacggcgacc cgttctcgaa cccgtgcccg
     4441 tcgctccaaa cggcgaactg cgacgacaac tgcaagatcg atcccaatcc gagccagtac
     4501 gacggcaacc acaacggctt cggcgacgca tgcgaccccg acgtcgacgg cgatggctac
     4561 tacgtcgaca ccgacaactg caatctgatc tacaacccga cgcagctcga ctcggacggc
     4621 gacggattgg gcgacgcctg cgacaagtgc cccgacgtac aggacaacgc caacgcctac
     4681 acgccggcga tccccgagct caacatcgat ccggccccgc tgcagcccga cagcgacggc
     4741 gacggaacac ccgacgcctg cgacgatcgg gcgtggctca cgatcggcgt gaagctcgac
     4801 ggcaagaact acagcaagtt cgccgacatc ccgcgtgatg gccgaccgca cccgatggag
     4861 ctcgccggtg cgccgggaag tggccagctg gtgcggctcg acgtcgacgc ctgcgacggc
     4921 ggtccggtcg accccgcgca gcgctaccag ctcgcgatgg gcgggctgcc cgcgaccgtc
     4981 gacgcatggc tcgaggacgg cgacgggcgc gcggtcgcgc gtgcgatccc gtccaacgtc
     5041 gccccgggcg agcgcggctt ccgcttccgc ccgcgttgcg accggcagta ccggctggtg
     5101 ctgcagacgg gtgacggttg gaacggctcg gcgacgttcc tgaccacggc cgccgtggtc
     5161 gacggcacct ctccctcgcc gtggctgccg atccccgcac cgtccgaccc gcccccgccg
     5221 attcccgacg ccgatgccga cggcgtgtgg gacgaggtcg acacctgccc gcaggtggcc
     5281 gacccgacca acgtcgacgc cgatctcgac ggcttcggcg acgcgtgcga cgtctgtccc
     5341 gccaccgcga accccgacca actgtcgttc gagttcgagc cgatcttcgc cacgtcgaag
     5401 acacagctgt cgtggacgac gcccgtcgac tacgactacg ccgtcggtcc gctcgacgac
     5461 gtggccaacc tcacgacgtg gctgaccggt ca
//
LOCUS       DASPGN010000363        15422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_301630, whole genome shotgun sequence.
ACCESSION   DASPGN010000363 DASPGN010000000
VERSION     DASPGN010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 15422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15422
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301630"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..596)
                     /locus_tag="VD788_10365"
     CDS             complement(87..596)
                     /locus_tag="VD788_10365"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HXV76710.1"
                     /translation="MSRLTAMDIEKQEFRTKLRGYDPDDVRLFLQSVAEEIERLNLDN
                     GTLREELGRSRQTIEEFRNREQTLQQTLVTAQSMSGEMKEKAKSEAELLIARARLSAE
                     RMLQQAQDQLARLETEIGRCRLERDLFERRLRSTIDEHLTLLDQRQEGAREIDNLHVL
                     PRRAGSDAG"
     gene            complement(823..1509)
                     /locus_tag="VD788_10370"
     CDS             complement(823..1509)
                     /locus_tag="VD788_10370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXV76711.1"
                     /translation="MNDSTVEDCYRAVRRRIEAACARADRDPREVRLVAITKTVPAET
                     IRRLLACGHHLLGENRVQEVLPKIDELGNEADWHFVGHLQRNKVRQVVGRFSLIHSLD
                     DTRLAAELDRRAGEAGLRQAVLLQVNAAGESTKFGVAPEEVGPVVDRVAAFGRLELRG
                     LMTVPPPAGEAEDSRRWFAGLRELRDRAAARSGLPLPELSMGMTDDFEVAIEEGATLI
                     RVGRALFGER"
     gene            complement(1506..1964)
                     /locus_tag="VD788_10375"
     CDS             complement(1506..1964)
                     /locus_tag="VD788_10375"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HXV76712.1"
                     /translation="MAERFRAAIDGGSRGNPGPAAWGVAVLGPDGEPAEGYAGYLGRA
                     TNNVAEYRALIEALELAIGRGADDVELLADSELVVRQIQGSYRVRNETLKPLFAEAMR
                     RIRAIPRFRIVHVRREQNTAADRLVNLALDRAEADPEAGTARIVEVGSGG"
     gene            complement(1948..2727)
                     /locus_tag="VD788_10380"
     CDS             complement(1948..2727)
                     /locus_tag="VD788_10380"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HXV76713.1"
                     /translation="MKAREQLLSLVRIQQLANEIRQAKGIVDAAPAQVEEIERRFRDR
                     NAEFVALKERNDELARDQRTRSAELAMLEEKKKKFMDDLMQVKNQKEYSAMLREIDAV
                     KAQIAGHEEAVLRDMEEIDKLERELVEYEEHIAKERDAVAIERREVEARAEEARIVIE
                     RRSAEREEVEGRLPSPLRAGLHRLEPRRQGIFVAEIENGVCQSCFVRVRPQVAQEIRT
                     ALVVHTCDSCKRFLYDRSLTSPPEEGVPAQRDVETANGGAV"
     gene            complement(2931..4232)
                     /locus_tag="VD788_10385"
     CDS             complement(2931..4232)
                     /locus_tag="VD788_10385"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HXV76714.1"
                     /translation="MLNAIFFFVVLVSVLLAAASGRMEALNLAIFEQAKAAVTLAINL
                     VGVMAFFLGLMRVAEDAGLLRVIDRMVGPVMRRLFPSVPPGHPAMSAMILNIAANMMG
                     LTNAATPFGIKAIEQLDTLNSRHGTATNDMVLFLAINTSGLAILPSGVAALRASLGST
                     DPWGIWITTMFASGCATIVGITAAWLYARLPGYRSSAPPPLTGPTASAPAAEPATAED
                     AVAPARSRASTALGVAFWVVFVMLLARFVWLETERMDGLDLFRSVTANWTVPALIAAI
                     LIFAWSRRVRVYESLVEGAKDGFQVALRIIPYLVAILVMVGMLRAAGGIELLVRFVGP
                     LTDGFGMPAEALPMALLRPLSGSGAFGIMAAIMEEHGPDSFIGYLVSTFQGSTETTLY
                     VLAVYFGAVGVKRSRHALAACLTADVAGILAAVFIVNLLFG"
     gene            complement(4285..4746)
                     /locus_tag="VD788_10390"
     CDS             complement(4285..4746)
                     /locus_tag="VD788_10390"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HXV76715.1"
                     /translation="MTKQQAFQRITQETIEEHRQINFYLDQITLTLDALDVASADVEP
                     MRRLAAQIEGLKERLVEHHLSEEKDGLFQAVLEAMPDCRVEISRLTKQHQRIIEILEM
                     ARIHAQHGEPSETESLREDLRNFLEMFRRHEYDEESLLKRALERENRAAID"
     gene            complement(4820..5485)
                     /locus_tag="VD788_10395"
     CDS             complement(4820..5485)
                     /locus_tag="VD788_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXV76716.1"
                     /translation="MKPHRSVPLDFTPLPEAEMVRRARRFAETMDRRRTVRHFSERDL
                     PADVVELAIRAAGTAPSGAHKQPWYFVAVRDAEVKRRIREAAEAEERASYEHRMPRQW
                     LDDLEPLGTDWRKPFLEICPWLIVVFAQSFGSTDEGRKRKHYYVQESVGIAVGILLTA
                     LHLSGVATLTHTPSPMGFLREILRRPENERAYLLIALGHPVEGCRVPDLLRKSRQEIL
                     EVV"
     gene            complement(5501..6979)
                     /locus_tag="VD788_10400"
     CDS             complement(5501..6979)
                     /locus_tag="VD788_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV76717.1"
                     /translation="MKRLLSTALMLAFHVGFARPVLQWFVGVRYRRRDRVPRGPCVVV
                     SNHNSHLDAAVLMSLFPLGRLPHVHPVAAADYFGSSWIRRTAAMVLMNGIPIERRPRA
                     GGDPLQPMADALAQGESLVFFPEGSRGEAGVVAPFRPGIGRLVQKMPGLLVVPVFLSG
                     PERIWPRGQVVPVPLSIDAFVGRPRSYSPELDAKEIADQLQRDVLALAPPAPPLPQPH
                     PGPPLRVAVTGLDPGSRHEVFDQVTRRLGQTEKTLGITDPVIEVDPDGVRELTGPIPQ
                     PRGRPWLGALAKIFRTGGLFKGQKFVEMVDLAQIDEALGHRPATRVVVTDGSALVDLL
                     AWAKAVYYPGTVDDNEMNHLLQYLAGRKPIPAAKWPSFIRRAPEVWLVNTFDLAHPPV
                     PDVLVHVRQPVERIMQLLRSRGERLAAHENEAFLERLDQSYGQIAKVLERRHKVDFIE
                     VELEARGDGEWLGVVQQVCRRRLEDRDTARRGDPDGNVPVVQ"
     gene            complement(6976..7872)
                     /locus_tag="VD788_10405"
     CDS             complement(6976..7872)
                     /locus_tag="VD788_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76718.1"
                     /translation="MSDSLSQFLADIPPLDVALVVAVGTVGLVSLESRRRGGRFRELP
                     GWAGFWAFAAVLLISLTHAPTYLSLPLLAAVMFVALRTYFFLAPVRPRDRYAILASYL
                     SIPFALWPVYLSNEVLFLVVVPVTLFLVVPVFLAIGHPEQRMLDSMGRTLLGVLLFVF
                     CLAHLGLLADHETGELLELFGILVLAAELPQRVAGRFRTGSGWWIKPTIGVPLSLVLA
                     AGLGWLLGPWSGLDPADAARAGALVSVAVTMGAVVNEAVSEDLALTAPSARRGRGALL
                     NRIMPAAYAAPVYFHYVNYFAP"
     gene            7927..9453
                     /gene="zwf"
                     /locus_tag="VD788_10410"
     CDS             7927..9453
                     /gene="zwf"
                     /locus_tag="VD788_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXV76719.1"
                     /translation="MGPTSTRTRGTDGLSQTRPADPCAVVIIGASGDLTRRKLIPALY
                     NLRLDGLLPERFAVVGTARSKTAHREFVDDLEQGIRSFSRQQFDAEQWRGFSAGLSYV
                     STGDGAGGYAELKQNLEQLDRSRGTAGNRLYYLSVPPSAIPAVIEGLRESGLNRPTST
                     GWTRIVIEKPIGHDLDSARRMNATVERAFKEPQIFRIDHYLGKETVQNLMVFRFANAI
                     FEPLWNQKYVDHVQVTVAESIGVEGRGAYYESAGATRDVLQNHMFQLLCLIGMEPPVS
                     LAPDAIRDEKVKVLEALRPVDPTRVAEASVRGQYAAGVIDGKEVVGYLAEEGVAPDSL
                     TETYVALRLYIDNWRWGGVPFYLRAGKRLNRRLTEIALRFNDVPHRLFPNDPPKPNTL
                     VLQVQPDEGINLVFDVKVPGAQPRIKPVEMEFSYRKEFGGEPSEAYERLLLDAILGDS
                     TLFIRRDEVEGSWRYVDRLLEGWARDEGPLPQYTAGTWGPAEAHVMLAADGRTWRRWR
                     "
     gene            complement(9472..11475)
                     /gene="tkt"
                     /locus_tag="VD788_10415"
     CDS             complement(9472..11475)
                     /gene="tkt"
                     /locus_tag="VD788_10415"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXV76720.1"
                     /translation="MSNVPDERLSRLAVNTIKFLSVDAVEKADSGHPGLPMGAADMAF
                     VLWSRFLRYDPKDPGWVDRDRFVLSAGHGCMLLYSLLHLAGYDLPLGELEHFRQWGSR
                     TPGHPEFGHTVGVEATTGPLGQGISNAVGMALSAKMLEARFNRDPEFAPISHRIFVLA
                     SDGDVMEGVSGEAGSLAGHLRLGNLVVLYDDNRITIEGETALAFSEDVGRRYDSYGWH
                     VQHASGHDHAELATAIAAAVDDKTRPSIIVCRTHIAHGAPTKQDSASAHGSPLGGEEV
                     AGAKLRADWPAEPTFRVPDDVRAFFRERAEEGSALRLAWEEKLDGWRRRNTAASSSWD
                     AIWRRSVPKDIVERLLAEAPAGAAATRAHGAVVLQRAAEYVPSLIGGSADLAPSTNTR
                     IKSSPSVGPGQFDGRNLHFGVREHAMGAMLNGFLYHGAFRPFGATFLVFSDYMRPPIR
                     LAALSKLPAIYVFTHDSIFVGEDGPTHEPVEQAWALRMIPNLHVFRPADGKETALAWG
                     MALQRKDGPTAMLLSRQKLPPIERRVKGELADPRRGAYLVAGHDRPQAILAATGSEVQ
                     LAIAAYDALSEEGRKLAVVSIPCVEIFHAQPSVYRQRLFPKGVPVATIEAGSTLPWRA
                     LTGPDGLTIGIDGYGASAPAAVNAEKYGMTTEAVTSKVRDWLL"
     gene            complement(11482..12513)
                     /gene="glk"
                     /locus_tag="VD788_10420"
     CDS             complement(11482..12513)
                     /gene="glk"
                     /locus_tag="VD788_10420"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HXV76721.1"
                     /translation="MILAADVGGTKTHLGLFRRSGARLTSVREQSFPSQEFASLEAIV
                     AEFVGSRTRTIDRCSVGVAGPVVGGRSQVVNLRWPVDANRLALRLGLDHVTLLNDLEA
                     NAWGIAHLPPRKLINLTPGVRSRGGNAALISAGTGLGMAILFPDGDRLRPSASEGGHQ
                     EFAPRDDLEIELLRFLRKRHGRVSIERVVAGPGFSAIYRFLIEHGWGRESAEMARRLA
                     AADDPNGVISEAGLEDRDPVARMTLQMFVSLFGAAAGDLALVSKSTAGVFVGGGIAPK
                     ILPELRRGEFVQSFRRKGRLTPLLEQIPVKVIVEPRTALIGAAAAATDPSTGSPGARR
                     SRNARRRRR"
     gene            complement(12537..>15422)
                     /locus_tag="VD788_10425"
     CDS             complement(12537..>15422)
                     /locus_tag="VD788_10425"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXV76722.1"
                     /translation="GIATVRIEQALLIPAEGIDGRTVEFVLQGSAWFTVEPPDEIEAG
                     QLELFTGHRRIDAPIETAVLSVADQRQVAALLDRYSVIELDAERLATAAATYAQWVGS
                     VERRATGAEAGMFKALYGDPVYRDYFALWCVTRGAGELVYTYDPGDAEQLTIASFNRP
                     QVRGWDRVRLERHIRFQQRKGRFLDTRIDDLGAWDIWLSTSWSPNGSDASGDHGFETR
                     HYDLDVRVSRDRMRLEGQATLELETTAAGRQTVSLELFRDLELDSVRDGLGRELFAFR
                     SGPEIVVGLPDRPAPGDRLTLHVGYHGRVLDWRGKGTFDLVTTDGWYPHCGSIDRATY
                     DVTLRWPRKLDLLAGGKLVASGEDGRYRWERRVLDVPSIAFTFVVGKYEISERQIDDT
                     RLRLAFSGTVGPPSPTMRERTVDVIARSLRFLEGAFGRYPLDELTVVVLPRGYSQSFL
                     GFIALNDTIAGAAEAEPFDAGRWKRETTIAHELAHQWWGNLVGWWSYRDQWLSEAMAN
                     YAGMLFHVHSAGGGDALLAELSAGWRTTLEQRIDDGRTVESLGPIVLGSRLNSSRAAN
                     GYGAIVYRKGAVVLAMLARAVGEERFLEIVRGILESHRGRVLQTEDFIAAVEQGAGLE
                     LDGFAGQYVYGTGIPFVYYDYDVDHAASGGSAWRLQGEARRLLEVRHTFHVVAGEDGR
                     WDVVRRLEPRGGGEGTAVMVPFRIVPSERAVDHPPGARPSQSGRLMLNGRAAEFEIES
                     KVEPDELIFDPWGEILAHFYSDESSPKRVARLRGQDLLVEGKPEEAERELLEALELPL
                     GTPRLSAGAAWRSDAVGAERDEDAAIHLILARLYIDGGQDERAEPHIRAADELLDGRP
                     WDRRLERDVLRARVDLRSGRAAAAYKRLKPTLGIAQSDPRHPRRDGARLRLQNERLAL
                     IEAYAVMAVAAYATGHATDYEWARDAALENGVDIGALAAVAKVGPRP"
BASE COUNT         2479 a         5422 c         5151 g         2370 t
ORIGIN      
        1 ccgaccggtc gccgcgccgg tcagggcgct gcggccggtg ggtgtggcga gttccgcgat
       61 tccgctgagc aggaggttcg cgcgtatcat ccggcgtcgg agccggcacg gcgcggcagc
      121 acgtggagat tgtcgatctc ccgcgctccc tcctgtcgct gatcgagcag cgtcaggtgc
      181 tcgtcgatcg tgctgcgcag ccggcgctcg aacagatccc gctcgagccg gcagcggccg
      241 atctcggttt cgagccgcgc gagctgatcc tgcgcctgct gcaacatgcg ctcggcgctg
      301 aggcgggccc gcgcgatcag cagctcggcc tcgctcttgg ccttctcctt catttcgccg
      361 ctcatgctct gcgccgtgac gagcgtttgc tggagggtct gctcgcggtt gcggaactcc
      421 tcgatcgtct gccggctccg accgagctcc tcgcgcagcg tgccgttgtc gagattgagc
      481 cgctcgatct cctcggcgac cgactggaga aacagacgca cgtcgtcggg gtcgtagccg
      541 cggagcttcg tgcggaactc ttgtttctcg atgtccatcg cggtcagtcg gctcatcgcg
      601 gaccctcccc ggtaggcgaa acgagaggaa acataggacc gccggccagg cttcgcaacc
      661 cgaacaacaa ggaccggccg gtgcatccct agccccttca acagtttagc gaacattccg
      721 tacgctcgtt cgcaggaccc ctaccgtcaa ccctgggtca ttctaacggg cagttcgccc
      781 gtcgcgtcaa gtttttcgac gggaagcggg acttgcggcc ggtcaacgct cgccgaacag
      841 ggcccggccg acacggatca aggtcgctcc ctcctcgatc gcgacctcga agtcatccgt
      901 catgcccatc gacagctcgg gcagcggcaa acccgatctc gcggcggcgc gatcgcgcag
      961 ctcgcgcaac ccggcgaacc agcgccggct gtcttccgcc tcgccggccg gcggcggcac
     1021 ggtcatcagg ccgcgcagct cgagccggcc gaaggcggcg acccggtcga cgaccggacc
     1081 gacctcctcc ggcgcgacgc cgaacttcgt cgactcgccg gccgcgttga cctgcagcag
     1141 cacggcctgg cgtagaccgg cctcacccgc gcggcggtcg agctcggccg ccagtcgggt
     1201 gtcgtccagg ctgtgaatca gcgagaaacg gccgacgacc tggcggacct tgttccgctg
     1261 gaggtggccg acgaagtgcc agtcggcctc gttccccagc tcgtcgatct tcggcagcac
     1321 ctcctgcacg cggttctcgc cgagcaggtg gtgaccgcag gcgagcagcc ggcggatcgt
     1381 ctccgccgga acggtcttgg tgatcgcgac caatcgcacc tcgcgcgggt ctcgatcggc
     1441 ccgtgcgcat gccgcctcga tccgtcgccg aacggcgcgg tagcaatcct cgaccgtgga
     1501 atcgttcatc ctccggaccc gacctccacg atccgggcgg tcccggcctc gggatcggcc
     1561 tcggcccggt cgagcgccag attgaccagg cggtcggccg ccgtgttctg ctctcgacgg
     1621 acgtggacga tgcgaaagcg gggaatcgcg cggatccggc gcatcgcttc ggcgaacagc
     1681 ggtttcagcg tctcgttcct cacgcgatag ctgccctgga tctggcgcac gaccagctcg
     1741 gagtcggcga gcagctcgac gtcgtccgct ccgcggccga tcgccagttc gagcgcctcg
     1801 atcagcgcgc ggtattcggc gacgttgttg gtcgcgcggc cgaggtagcc ggcgtatccc
     1861 tcggccggct cgccgtccgg acccaggacc gccacgcccc aggcggcggg tcccgggttc
     1921 ccgcgggagc caccgtcgat cgccgcccta aaccgctccg ccattcgccg tttcgacgtc
     1981 gcgctgcgcg ggaacaccct cctcgggagg ggaagtcagc gagcggtcgt agaggaatct
     2041 cttgcagctg tcacaggtgt ggacgacgag cgcggtgcgg atctcctgcg cgacttgggg
     2101 tcggacgcgg acgaaacagc tttggcagac gccgttctcg atctcggcga cgaagatccc
     2161 ctgacggcgc ggctccagcc ggtgcaggcc ggcgcgcagc ggcgacggca gacgcccctc
     2221 gacctcctcg cgctcggcac tgcggcgctc gatcacgatc cgcgcttcct cggcacgcgc
     2281 ctcgacctcc cgccgctcga tcgcgacggc gtcacgctcc ttggcgatgt gctcttcgta
     2341 ctcgaccagc tcgcgctcga gcttgtcgat ctcttccata tcgcgcagga ccgcttcctc
     2401 gtgcccggcg atctgcgcct tgaccgcatc gatctcccgc agcatggccg agtattcttt
     2461 ctggttcttg acctgcatca ggtcgtccat gaacttcttc ttcttttcct cgagcatggc
     2521 caattccgcc gagcgcgtgc gctggtcgcg cgcgagctcg tcgttgcgtt ctttcaacgc
     2581 gacgaactcg gcgttgcggt cgcggaatcg ccgctcgatc tcctcgacct gagccggagc
     2641 ggcgtcgacg atccccttgg cctggcgaat ctcgttggcc agctgctgga tacgaaccag
     2701 actcagcaac tgctccctgg ccttcaaaac cggccctcct tccgccgccg cgattcgtac
     2761 cccaaaaaca cgaagggcgc tccgatctcg agcgcccttc tcatcgcggt cccgtcgcgc
     2821 ccgctgcgtc gccgcagcgg tcccgcagga cggatgccac ctcgcggctt gtcgttgctc
     2881 atcgtcgatg ccttcacaaa ccgttaacgt accagccctc cccgggagcg tcaaccgaag
     2941 agcaggttga cgatgaagac cgcggccaga atgccggcaa cgtcggcggt caggcaggcg
     3001 gccagggcgt gccggctgcg tttgaccccg accgctccga aatagacggc gagcacgtag
     3061 agcgtggtct cggtgctgcc ctggaaggtg ctgaccaggt agccgatgaa cgaatcgggc
     3121 ccgtgctcct ccatgatcgc ggccatgatg ccgaacgccc ccgatcccga cagcggtcgc
     3181 agcaaggcca tcggcagcgc ctcggccggc ataccgaagc cgtccgtcaa cgggccgacg
     3241 aagcggacca gcagctcgat cccgccggcg gcgcgcagca tgccgaccat caccaggatc
     3301 gcgacgagat aggggatgat gcgcagggcg acttggaaac catccttggc gccttcgacc
     3361 agcgactcgt agacgcgcac cctccgagac caggcgaaaa tcaggatcgc ggcgatcagc
     3421 gcgggtaccg tccagttggc ggtcaccgag cggaacaggt cgagtccgtc catccgctcc
     3481 gtctcgagcc agacgaaccg cgccagcagc atcacgaaga cgacccagaa cgcgacgccc
     3541 agcgccgtgc tcgcgcggga gcgtgccggg gcgacggcat cctcggcggt ggccggttcg
     3601 gccgccggcg cgctcgcggt cggacccgtg agcggtggag gcgcgctcga ccggtagccc
     3661 ggcaggcggg cgtagagcca agccgcggtg atcccgacga tcgtcgcgca gccgctggcg
     3721 aacatcgtcg tgatccaaat accccacggg tcggtcgagc cgagcgaggc ccgcagcgcg
     3781 gcgacaccgg acggaaggat cgccagtccg gaggtgttga tcgcgagaaa cagcaccatg
     3841 tcgttcgtcg cggtgccgtg ccggctgttc aaggtgtcga gctgctcgat cgccttgatg
     3901 ccgaacggcg tggcggcgtt ggtcagtccc atcatgttgg cggcgatgtt gaggatcatc
     3961 gcgctcatcg ccggatggcc gggcggcacc gacggaaaca gccggcgcat caccgggccg
     4021 accatccggt cgatcacccg gagcaagccc gcgtcctcgg cgactctcat cagcccgaga
     4081 aagaaggcca tcacgccgac caggttgatc gccagcgtga ccgcggcctt ggcctgctcg
     4141 aagatcgcga ggttgagcgc ttccatgcgc ccgcttgcag cggcgagcag gaccgagacg
     4201 agcacgacga agaagaagat cgcgttcagc aacggatcag ctttccgtgt gaagcgggaa
     4261 gtccgtcacg gcccggtcgt gcggtcagtc gatcgcggcg cggttctcgc gctcgagcgc
     4321 ccgtttgagc agcgactctt cgtcgtactc gtgtcgtcgg aacatctcga ggaagttccg
     4381 caggtcctcg cgcagcgatt cggtctcgct cggctcgccg tgctgggcat ggatccgcgc
     4441 catttcgagg atctcgatga tcctctggtg ctgcttggtc agtcggctga tctcgacgcg
     4501 gcaatcgggc atcgcctcca gcacggcctg gaacagaccg tccttctcct cgctaaggtg
     4561 gtgctcgacg aggcgctctt tcaagccctc gatctgcgcg gccagccgcc gcatcggttc
     4621 gacgtcggcc gacgcgacat cgagcgcgtc caacgtcagc gtgatctggt cgagatagaa
     4681 gttgatttga cgatgctctt cgatcgtttc ctgggtaatc cgctgaaatg cttgttgttt
     4741 cgtcatgtcg ccctgactcc ctgctccacg ttgaaagccc cttcaagtat cgttcctgtc
     4801 aagtcggcgt gcaaaaccgt cagacgacct cgaggatctc ttgccggctc ttgcgcagca
     4861 gatccggcac ccggcagccc tcgaccgggt ggcccagcgc gatcaacagg taggcccgct
     4921 cgttctccgg ccgtctcagg atctcccgca gaaagcccat cgggctcggc gtatgggtca
     4981 gcgtcgccac gccggacagg tgcaacgccg tcaacaagat gccgacggcg atgccgaccg
     5041 attcctggac gtagtagtgc ttgcgcttcc ggccctcgtc cgtgctgccg aagctctgcg
     5101 cgaagaccac gatcagccac gggcagatct cgaggaacgg cttgcgccag tcggtgccga
     5161 gcggttcgag atcgtcgagc cactgccgtg gcatgcgatg ctcgtagctg gcgcgctcct
     5221 cggcctcggc cgcttcccgg atccggcgtt tgacctccgc gtcgcgcacc gcgacgaagt
     5281 accacggttg cttgtgggct cccgatggcg cggtgccggc ggcgcgaatc gccagctcga
     5341 cgacgtcggc cgggaggtct cgctccgaga aatgacggac ggtccggcgg cggtccatcg
     5401 tctcggcgaa tcggcgcgcc cggcgcacca tctccgcctc gggcagcgga gtgaaatcga
     5461 gtgggaccga acgatgtggt ttcacggcga ccttccgcgc tcattgtacg acgggaacgt
     5521 tcccgtccgg atcgccgcgt cgcgcggtat cgcggtcttc gagccgccgg cggcacacct
     5581 gctggacgac gccgagccat tcgccgtccc cgcgggcctc gagctcgacc tcgatgaaat
     5641 cgaccttgtg ccgccgttcg agcaccttgg cgatctggcc gtagctctgg tcgaggcgct
     5701 cgagaaacgc ctcgttctcg tgcgcggcca atcgttcacc gcgggaacgg agcagctgca
     5761 tgatgcgctc gaccggttgg cggacgtgga ccagcacgtc cggcacgggc gggtgggcca
     5821 gatcgaaggt gttgaccaac cacacctccg gtgcgcggcg gatgaagctc ggccacttcg
     5881 cggccggtat cggtttgcgt cccgcgaggt actgcagcaa gtggttcatc tcgttgtcgt
     5941 cgaccgtacc ggggtagtag accgccttgg cccaggccaa caggtcgacg agcgcgctgc
     6001 cgtcggtcac cacgacccgg gtcgccggcc ggtggccgag ggcctcgtcg atctgcgcca
     6061 ggtcgaccat ctcgacgaac ttctgtccct tgaacagacc gccggtgcgg aagatcttcg
     6121 ccagtgcgcc gagccacggc cgtccgcgcg gctgcgggat cggaccggtc agctcgcgga
     6181 ccccatcggg gtcgacctcg atgaccggat cggtgattcc gagcgtcttt tcggtctggc
     6241 ccagacgtcg cgtgacctga tcgaacacct cgtgacgcga gcccgggtcg agaccggtga
     6301 cggccacgcg caacgggggt cccgggtgcg gctgcggcag cggcggcgcg ggcggagcga
     6361 gcgccagcac gtcgcgttgc aactggtcgg cgatctcctt cgcgtccagc tccggcgagt
     6421 agctgcgcgg ccggccgacg aacgcgtcga tgctgagcgg taccggcacg acctgccccc
     6481 gcggccagat ccgctcgggg ccggagagga acaccgggac gaccaacagt ccgggcatct
     6541 tctgcaccag acggccgatg ccgggacgga acggcgcgac gacgccagcc tcgccgcggc
     6601 tgccctcggg gaagaagacc aggctctcac cctgagccag cgcgtcggcc atcggctgca
     6661 gcgggtcccc accggcgcgc ggccggcgct cgatcgggat gccgttcatc agcaccatcg
     6721 cggcggtgcg ccggatccag ctcgagccga agtagtcggc ggcggcgacg ggatggacgt
     6781 gcggcagacg cccgagcgga aacaggctca tcagcaccgc ggcgtcgaga tgcgagttgt
     6841 ggttcgacac cacgacgcac gggccacgag gcacgcgatc gcggcggcgg tagcgcacgc
     6901 caacgaacca ctgcaggacc ggtcgcgcga agccgacgtg gaacgcgagc atcaacgcgg
     6961 tcgacagcag gcgtttcacg gagcgaagta gttgacgtag tgaaagtaga ccggcgcggc
     7021 ataggccgcc ggcatgatcc ggttgagcag tgcgccacgc ccgcgtcgcg ccgacggcgc
     7081 cgtcagcgcc agatcctccg acaccgcctc gttgaccacc gcccccatcg tgacggcgac
     7141 gctgaccaac gccccggcgc gcgccgcgtc cgccggatcg agaccggacc acggaccgag
     7201 cagccagccc agaccggcgg ccaacaccag gctgagtggc acgccgatcg tcggcttgat
     7261 ccaccagccg ctgccggtgc ggaaccgtcc ggcgacgcgc tgcggcagct ccgcggccag
     7321 caccaggatg ccgaacaact cgagcagctc gcccgtctcg tgatcggcca gcagcccgag
     7381 gtgcgccagg cagaacacga acagcagcac gccgagcagc gtgcgcccca tcgaatcgag
     7441 catgcgttgt tccgggtggc cgatcgcgag gaaaaccggg acgacgagaa acagggtgac
     7501 ggggacgacg acgagaaaca acacctcgtt gctcaggtag accggccaca gtgcgaacgg
     7561 gatcgacaag tacgacgcga ggatcgcgta gcgatctcgc ggacggaccg gcgccaggaa
     7621 gaagtaggtg cggagcgcaa cgaacatcac cgcggcgagg agcggcagcg aaaggtaggt
     7681 cggagcgtgg gtcaggctga tcagcaacac cgcggcgaag gcccagaaac cggcccaccc
     7741 cggcagctcg cgaaaccgac cgccgcgccg gcgcgactcg agcgaaacga gtccgacggt
     7801 ccccaccgcg accaccaggg ccacgtcgag cgggggaatg tcggcgagga actggctcag
     7861 cgagtcggac acggcgttat tatgacgtcg aacccggtcg gcagaccaaa gaatcgaggt
     7921 actcgaatgg gccctacctc cacgcgcacg cgcggcaccg acgggctgtc ccagacgcgc
     7981 cccgccgacc cgtgcgccgt cgtgatcatc ggtgccagtg gcgatctcac acggcgaaag
     8041 ttgatccccg ccttgtacaa cttgcgactc gacggcctgc tgcccgagcg attcgccgtg
     8101 gtcggcacgg cgcgcagcaa gaccgcgcac cgggagttcg tcgacgatct ggaacaaggg
     8161 atccgctcgt tctcgcgcca gcagttcgat gccgagcagt ggcgcggctt ctccgccggg
     8221 ttgtcctacg tcagcacggg cgacggcgcc ggtggctacg ccgagctgaa gcagaacctc
     8281 gaacagctcg accggtcacg cggcacggcg ggcaaccggc tctactacct gtcggtgccg
     8341 ccatcggcga ttcccgccgt gatcgaaggg ttgcgcgaga gcgggttgaa tcggccgacc
     8401 tccaccggct ggacgcgcat cgtgatcgag aagccgatcg gccacgacct ggactccgcg
     8461 cgtcgaatga acgcgaccgt cgagcgcgcg ttcaaggagc cgcagatctt ccgcatcgat
     8521 cactacctgg gtaaggagac cgtgcagaac ctgatggtct tccgcttcgc caacgcgatc
     8581 ttcgaaccgt tgtggaatca gaaatacgtc gaccacgtgc aggtcaccgt cgccgagtcg
     8641 atcggcgtcg aggggcgcgg cgcctattac gagtcggccg gagcgacgcg ggacgtgctg
     8701 cagaaccaca tgttccagtt gctgtgcctg atcgggatgg agccgccggt ctcgttggcg
     8761 ccggatgcga tccgcgacga gaaggtcaag gtgctcgagg cgctgcgccc ggtcgacccg
     8821 acgcgggtcg ccgaggcgag cgtgcgcggc cagtacgcgg ccggagtgat cgacggcaag
     8881 gaggtcgtcg gctacttggc ggaggagggc gtcgcgccgg attcgctgac cgagacctac
     8941 gtagccctgc ggctctacat cgacaactgg cgctggggcg gcgtgccgtt ctacctgcgc
     9001 gcgggaaaac ggctgaaccg ccggctgacc gagatcgcgc tgcgcttcaa cgacgtgccc
     9061 caccgtctgt ttccgaatga cccgccgaag ccgaacacgc tcgtgttgca ggtccaaccg
     9121 gacgagggca tcaatctcgt cttcgacgtc aaggtacccg gagcccagcc acggatcaaa
     9181 ccggtggaga tggagttcag ctaccgcaag gagttcggcg gtgaaccgtc ggaggcctac
     9241 gaacgactgc tgctcgatgc gatcctcggc gactctaccc tgttcatccg gcgcgacgag
     9301 gtcgagggct cgtggcgtta cgtcgaccgc ttgctcgagg gctgggcccg cgacgagggc
     9361 ccgctgcccc agtacaccgc cggcacctgg gggccggccg aggcgcacgt gatgctcgcc
     9421 gccgacgggc gcacctggcg gcgttggcgc tgacgccgtc gcgccgcacg atcagagcag
     9481 ccagtcgcgg accttggatg tcaccgcctc ggtcgtcata ccgtatttct ccgcgttgac
     9541 cgctgccggc gccgacgcgc cgtagccgtc gatgccgatc gtcaaaccgt ccggtccggt
     9601 gagcgcacgc cacggcagcg tggagccggc ctcgatcgtc gcgaccggga cgcccttggg
     9661 gaacagccgt tgccggtaga ccgacggctg tgcgtggaag atctcgacgc acggtatcga
     9721 cacgacggcc agcttgcggc cctcctcgga cagcgcgtcg taggctgcga tcgccagctg
     9781 gacctccgat ccggtcgccg ccaggatcgc ctgtgggcga tcgtggccgg cgacgaggta
     9841 cgccccgcgc cgcgggtcgg ccagctcacc cttcacccgc cgctcgatcg gcgggagctt
     9901 ctgacgcgac agcagcatcg cggtcggacc gtccttgcgc tgcaacgcca tcccccaggc
     9961 gagcgcggtc tccttgccgt cggccgggcg aaagacgtgt aggttcggga tcatccgcag
    10021 cgcccacgcc tgttcgaccg gttcgtgcgt cgggccgtcc tcgccgacga agatcgaatc
    10081 gtgagtgaag acgtagatcg ccgggagctt cgacaacgcc gccagccgga tcggcgggcg
    10141 catgtagtcc gaaaagacga ggaacgtcgc gccgaacggg cggaacgcgc cgtgatacag
    10201 gaaaccgttg agcatcgctc ccatcgcgtg ctcgcggacg ccgaaatgaa gattgcgccc
    10261 gtcgaactgc ccgggaccga ccgacggcga gctcttgatc cgcgtgttgg tcgacggcgc
    10321 caggtcggcc gagccgccga tcagcgacgg cacgtactcg gccgccctct gcaataccac
    10381 ggcgccgtgg gcgcgcgtcg cggcggcgcc cgcgggcgcc tccgccaaca accgctcgac
    10441 gatgtccttc ggcacactgc gccgccagat cgcgtcccac gaactcgagg ccgcggtgtt
    10501 gcgccggcgc catccgtcga gcttctcctc ccaagccaga cgcagcgccg agccctcctc
    10561 ggcccgctcg cggaagaacg cgcggacgtc gtccggcacg cggaaggtcg gctccgccgg
    10621 ccaatcggcc cgaagcttgg ccccggcgac ctcctcgccc cccagcggcg agccgtgcgc
    10681 ggatgccgag tcttgcttgg tcggcgcacc gtgggcaatg tgcgtgcgac agacgatgat
    10741 cgacggccgg gtcttgtcgt cgaccgcggc agcgatcgcc gtggccagct ccgcatgatc
    10801 gtgcccggag gcgtgctgga cgtgccagcc gtagctgtcg tagcgccggc cgacgtcttc
    10861 gctgaacgcg agcgcggtct ccccttcgat cgtgatccgg ttgtcgtcgt acagcacgac
    10921 gaggttgccg agccgcaggt gaccggccag cgagccggcc tctccgctca ccccttccat
    10981 cacgtcgccg tcgctggcca gcacgaagat gcggtgggag atcggcgcga actcggggtc
    11041 gcggttgaag cgcgcctcga gcatcttggc ggacagggcc atcccgaccg cgttggagat
    11101 cccctgcccg agtggtccgg tcgtcgcctc gaccccgacc gtgtggccga actcggggtg
    11161 tcccggcgtg cggctgcccc attgcctgaa gtgctcgagc tcgccgagcg gtaggtcgta
    11221 gccggcgaga tgcagcaacg agtagagcag catgcagccg tgaccggccg acaacacgaa
    11281 gcgatcgcgg tcgacccagc cgggatcctt cgggtcgtag cgcaaaaagc ggctccagag
    11341 gacgaacgcc atgtcggcgg cgcccatcgg cagcccgggg tggcccgagt cggccttctc
    11401 caccgcgtcg acggacagga acttgatcgt gttgaccgcc agtcggctca atcgctcgtc
    11461 gggtacgttg gacatcggcg cctacctcct tctgcgacgg gcgtttcgcg accggcgggc
    11521 gcctggcgac ccggtcgacg ggtcggtggc agcggcggcg gcaccgatca gcgcggtgcg
    11581 cggctcgacg atcaccttga ccgggatctg ctcgagcagc ggggtcagcc ggcccttgcg
    11641 acgaaaggac tgcacgaact ctccgcggcg cagctcgggc aggatcttcg gcgcgatgcc
    11701 gccgccgacg aagacgcccg cggtcgattt cgacaccagc gccagatcgc ccgccgccgc
    11761 gccgaacaag gaaacgaaca tctgcagcgt catccgcgcg accggatcgc ggtcttccag
    11821 accggcctcg ctgatcactc cgttcgggtc gtccgccgcg gcgagccgac gagccatctc
    11881 cgccgactcc cggccccatc cgtgctcgat cagaaagcgg tagatcgcgg agaacccggg
    11941 tccggcgacg acccgctcga tgctgacccg gccgtggcgc ttgcgcagga agcgcaacag
    12001 ctcgatctcg agatcgtcgc gcggcgcgaa ctcctggtgc ccgccctcgc tcgcgctcgg
    12061 ccgcaaccgg tcgccgtcgg ggaacaggat cgccatgccg agcccggtgc cggccgagat
    12121 cagcgcggcg ttgcccccgc gcgatcgcac gcccggtgtg aggttgatca acttgcgcgg
    12181 cggcaggtgc gcgatgcccc aggcgttcgc ctcgaggtcg ttcaacaggg tcacgtgatc
    12241 gaggccgagc cgcagggcga gcctgttcgc gtcgaccggc cagcgcaggt tgaccacctg
    12301 gctgcggccg ccgacgaccg gacccgcgac gccgaccgag cagcgatcga tcgtcctggt
    12361 tcgtgagccg acgaactcgg cgacgatcgc ctcgagcgag gcgaattcct gactcgggaa
    12421 gctctgttcg cgtaccgagg tcagccgggc accggatcga cgaaacagac cgaggtgcgt
    12481 cttggtgccg ccgacatccg ccgcgaggat catccgccga ttatacgcaa cgcggactac
    12541 ggtcgcggcc cgaccttggc taccgcggcc agcgcaccga tgtcgacgcc gttctccaac
    12601 gcggcgtcgc gcgcccactc gtagtcggtc gcgtgaccgg tggcgtaggc ggcgaccgcc
    12661 atcacggcgt aggcttcgat cagcgccagc cgttcgttct gcaggcgcag ccgcgcgccg
    12721 tctcgacgcg ggtgacgcgg gtccgactgt gcgatgccga gcgtcggctt caaccgcttg
    12781 taggccgcgg ccgcccgccc cgatcgcaag tcgacgcgtg cccgaaggac gtcgcgctcg
    12841 agccggcgat cccacggcct cccgtcgagc agttcgtccg ccgcgcggat gtgcggctcg
    12901 gcgcgctcgt cctgtccacc atcgatgtag agccgggcga ggatcaggtg gatcgcggcg
    12961 tcctcgtcgc gctccgcgcc caccgcgtcg gagcgccacg cagccccagc gctcaaccgc
    13021 ggcgttccca acggtagctc gagcgcctcg agcagctcgc gctcggcttc ctcgggcttg
    13081 ccctcgacga gcaggtcttg gccgcgcaag cgcgccaccc gcttgggact gctttcgtcg
    13141 gaatagaagt gcgccaggat ctcgccccat gggtcgaaga tcagctcgtc cggttcgacc
    13201 ttcgactcga tctcgaactc cgccgcccgc ccgttcagca tcagccggcc ggactgcgac
    13261 gggcgggcgc cgggcgggtg gtcgaccgcg cgctcgctcg gcacgatgcg gaacgggacc
    13321 atcaccgcgg taccctcacc tccgccgcgc ggctcgagcc gccggacgac gtcccaccgg
    13381 ccgtcctcgc ccgcgaccac atggaaggtg tgtctgacct cgagcagacg ccgcgcctcg
    13441 ccttgcagac gccaggcgga cccgcccgag gcggcatggt cgacgtcgta gtcgtagtac
    13501 acgaacggga tgccggtacc gtagacgtac tggccggcga agccgtcgag ctcgagaccg
    13561 gcaccctgct cgacggcggc gatgaagtcc tcggtctgga gcacgcgacc gcggtgggac
    13621 tccaggatcc cgcgcacgat ctcgagaaac cgctcctcgc cgacggcgcg ggccagcatc
    13681 gccagcacga cggcgccctt gcggtagacg atcgccccgt agccgttcgc cgcgcgggac
    13741 gagttgagcc gactgccgag cacgatcggc cccaacgact cgaccgtgcg gccgtcgtcg
    13801 atccgttgct cgagcgtcgt acgccagccc gcggacagct cggccagcag ggcgtcgccg
    13861 ccgcccgcgg aatgaacgtg gaacaacatg cccgcgtagt tcgccatcgc ctcgctcaac
    13921 cactggtcgc ggtagctcca ccacccgacg aggttgcccc accactggtg cgccagctcg
    13981 tgggcgatcg tcgtctcgcg cttccaacgg ccggcgtcga acggctccgc ttcggccgcg
    14041 cccgcgatcg tgtcgttcaa ggcgatgaac ccgagaaagc tctgcgagta tccgcgcggg
    14101 agcaccacga ccgtcagctc gtcgagcggg taccgcccga aagcgccttc gagaaaccgg
    14161 agcgatcgcg cgatgacgtc gacggttcgc tcgcgcatcg tcggcgacgg agggccgacg
    14221 gtgccgctga acgccagccg caggcgggtg tcgtcgatct gccgctcgct gatctcgtac
    14281 ttgccgacga cgaaggtgaa cgcgatcgac ggtacgtcca gcactcggcg ctcccagcgg
    14341 tagcgaccgt cctcgccgga ggcgacgagc ttcccgccgg cgagcagatc gagctttcgc
    14401 ggccagcgca acgtcacgtc gtaggtcgcg cggtcgatcg acccgcagtg cggataccag
    14461 ccgtcggtcg tgaccagatc gaatgttcct ttgccccgcc agtcgagcac ccgcccgtgg
    14521 tagccgacgt gcagtgtcaa ccggtcgccc ggcgccggtc tgtccgggag gccgacgacg
    14581 atctcgggtc ccgaacgaaa agcgaacagc tcccgtccca gcccgtcccg cacgctgtcc
    14641 agctcgaggt cgcggaacag ctcgagcgac accgtctgcc ggccggctgc ggtcgtctcg
    14701 agctccagcg tcgcctggcc ttccaaccgc atccgatccc ggctgacgcg gacgtcgaga
    14761 tcgtagtgac gcgtctcgaa accgtggtcg cccgaggcgt cggagccgtt cggcgaccac
    14821 gacgtcgaca accagatgtc ccacgcgccg agatcgtcga tgcgcgtgtc gaggaagcgg
    14881 cccttgcgtt gctggaagcg gatgtgacgc tcgaggcgaa cccgatccca gccgcgaacc
    14941 tgcggccggt tgaacgaggc gatcgtcagc tgttcggcgt ctcccggatc gtaggtatag
    15001 accagttcgc ccgcgccgcg ggtcacgcac cacagcgcga agtaatcgcg gtagaccgga
    15061 tcgccgtaga gcgccttgaa catgccggcc tcggccccgg tcgcacgccg ctcgacgctg
    15121 ccgacccact gcgcgtaagt cgccgcggcg gtcgcgagcc gctcggcgtc gagctcgatc
    15181 accgagtagc ggtcgagcag tgctgctacc tgccgctgat cggccacgct caggaccgcg
    15241 gtctcgatcg gcgcgtcgat ccggcggtgc ccggtgaaca actcgagctg cccggcttcg
    15301 atctcgtccg gtggttcgac ggtgaaccac gccgaaccct gcagcacgaa ctcgaccgtg
    15361 cggccgtcga tcccttcggc gggaatcaac agcgcctgtt cgatcctcac cgtggcgatc
    15421 cc
//
LOCUS       DASPGN010000364         5573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_65961, whole genome shotgun sequence.
ACCESSION   DASPGN010000364 DASPGN010000000
VERSION     DASPGN010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65961"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VD788_10430"
     CDS             complement(<1..373)
                     /locus_tag="VD788_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76723.1"
                     /translation="MVPPADLVFRDVTPSRWNDVQTLFGERGACGGCWCMFWRLPRKR
                     WESGKGGPNRRALGRIVTSGGRPGVLAYDRRRPVGWCAIAPREVYPSLARSRVLAPVD
                     DTPVWSVSCLFVLKDYRRQGLS"
     gene            complement(389..1636)
                     /locus_tag="VD788_10435"
     CDS             complement(389..1636)
                     /locus_tag="VD788_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76724.1"
                     /translation="MKQNQVVDRPGPTSILRRNLTMSSSCMLGVALLLWPVTPTAADG
                     PFPPHGLAALVGEAVAATEARITPGALHADVAAVQTELARIIGRMGPGPASYVRARRL
                     HRLLHRRYLAQYRRDADSLTRLFDRGEFNCLSATLLYGLVARELGYRVVVIERPGHLL
                     LALDLDGRRVMIETTSVGGFDRHPPGADPSSLNLFGLERAAATGGDPTVIEREFPLEA
                     MIGFAWLNAAWRDLEAGRPRLSSRRVDRARGFLPYVAGTDDARRILARAFHGEYESAR
                     FESALEIARIEFGMWPQTTTARERLLAAAVKRIEEVCDADLVVDADRIAREVTGSASG
                     ADVRRFEQRVWPLVAASAVRLSDWGLAERSAERYARVEPDRVEARRLLDWVAERRDLA
                     GQRSSSPVCEEHPTRADTSTSRR"
     gene            complement(1753..2469)
                     /locus_tag="VD788_10440"
     CDS             complement(1753..2469)
                     /locus_tag="VD788_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV76725.1"
                     /translation="MMNAKTQSARVVVVEDEPDIAEFLRGALTKDGFRATAETTAQGA
                     LAAIRRDNVDLVILDLMLPDMSGLELLKMLKRDEATAGARVIVVSARTEEVDRVLAFE
                     FGADDYVTKPFSPRELMLRVKAVLQRSAEAGPRPEELITAGPIEIDMQMHEARVEGRP
                     IKLTLTEFRLLAELVRASGRVRSREALLSEVWGYDSEVMSRTVDTHIRRLRNKLGNAA
                     NWLDTVRGIGYRIQPPGPEA"
     gene            complement(2582..4021)
                     /locus_tag="VD788_10445"
     CDS             complement(2582..4021)
                     /locus_tag="VD788_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76726.1"
                     /translation="MRRTIPFALLPLLAVTCGLAGLPSGEGTGDDGSNPVALERWTDP
                     TFQKEFLGSYGFQAELEPRVTSVEREKMEEILRLMSGGSDTDAIEELERYLAPPASNT
                     KKKRDPDFDGTTSAIFDFTLGNLYFQREELERAAASYENAIAKFPSFRRAHKNLGLIR
                     VRQGDFDKAVDSLSEVIELGGRDGLTFGLLGYAYSSTGQFVSAETAYRNAVLLQPETL
                     DWKLGLTQSVFKQQKFGEAVTLCEELIETYPERADYWILQANAYIGLGRPLAAAENFE
                     IVQRMGKATVPSLYMLGDIYVNEGLWDLALRAYGLAVAGNPQQPVTQPMQRVEVLAQR
                     GALDQAKALLSEVKRVFGDRIEADDKKKLLKLEARVAVADGEGGASVGVLEEIVAIDP
                     LDGDALILLGQHYAKQQEPERAIFYYERAESIEEFEAEAKVRHAQLLVELSRYEAAVP
                     LLKRAQELEPRDDVANYLEQVERIARSRR"
     gene            complement(4053..4721)
                     /locus_tag="VD788_10450"
     CDS             complement(4053..4721)
                     /locus_tag="VD788_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXV76727.1"
                     /translation="MRQGSARRSISLLQGAGAFCLAVGATLALFLVLPLMQSIYDPIP
                     DDLLSVTEVGVELPPPPPPLPEEETEEEEPPDEPPKLNEPAQPLDLAQLDLALNPGLG
                     DGVFGDFTVELFDRMAQGGDEELDRVFSLGELDQRPRVIFQRMPQYPPELRRSKRQGT
                     VYVVFVVDTDGRVTSPTIETASDGAFERPALEAVRQWRFEPGTRKGEKVQFKMRVPIT
                     FNAG"
     gene            complement(4741..5151)
                     /locus_tag="VD788_10455"
     CDS             complement(4741..5151)
                     /locus_tag="VD788_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV76728.1"
                     /translation="MGRFRSFSGREEEAAGIDISPLLDAVFILLIFFIVTTTFVEESG
                     VEVDKPQAASAVKLEKNSILIALTADGAVVYGGREIGIAGVQPLVERMLQKEDLPVIV
                     QADSSVQAGLMVRVIDEAKLGGATKVSVATRKQA"
     gene            complement(5164..>5573)
                     /locus_tag="VD788_10460"
     CDS             complement(5164..>5573)
                     /locus_tag="VD788_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV76729.1"
                     /translation="FAMGATDLKEVAVRFAELDSTELAPFDRDLRFMRRAVSTAPLFG
                     LLGTVTGMLATFAALATGSGGDKTMDLIAGGISEALITTETGLVIALPGLFFQFHLTR
                     MRDRYEAFLAHLETVCTQRFYRRLRAARDVEAA"
BASE COUNT          907 a         1951 c         1849 g          866 t
ORIGIN      
        1 acgagagacc ctggcgtcga tagtccttca gcacgaacag gcacgacacc gaccaaacgg
       61 gtgtatcgtc gacgggcgcg aggacgcggg agcgggcgag cgacgggtag acctcgcgcg
      121 gtgcgatggc gcaccacccg acggggcgcc gtcggtcgta ggccaggacg ccgggacgcc
      181 caccggaggt gacgatgcgt ccgagggcgc ggcggttggg cccgcccttg ccgctttccc
      241 atcgcttgcg gggaaggcgc cagaacatgc accagcatcc gccgcaggca ccgcgttcgc
      301 cgaacagggt ctgaacgtcg ttccagcgcg acggggtcac gtcgcggaac accagatctg
      361 cggggggcac cacgaaccgg attgtatatc agcgccgcga ggtcgacgtg tcggcccggg
      421 tcgggtgctc ttcgcagacg ggcgacgacg aacgctggcc ggcgagatct cggcgctcgg
      481 cgacccagtc gagcaggcgg cgggcctcga cgcggtccgg ctcgacacgg gcgtagcgct
      541 cggccgaccg ctcggccaac ccccaatcgg acagccgtac cgcggaggcc gcgacgagcg
      601 gccacacccg ctgctcgaag cgcctcacgt ccgcaccgct cgcactcccc gtcacctcgc
      661 gggcgatccg gtcggcgtcg acgacgagat cggcgtcgca cacctcctcg atccgtttga
      721 cggcggccgc caagagccgc tcgcgcgccg tcgtggtttg cggccacata ccgaactcga
      781 tgcgggcgat ctcgagtgcc gactcgaacc gcgccgattc gtactcgcca tgaaaagcgc
      841 gggccaggat ccgtcgcgcg tcgtcggtgc cggcgacgta cggaagaaaa ccacgcgcgc
      901 ggtcgacccg ccgtgacgac agccgcggtc ggcccgcctc gagatcccgc caggcggcgt
      961 tgagccaggc gaagccgatc atcgcttcga gcgggaactc gcgctcgatc accgtcggat
     1021 cgccgccggt cgccgccgct cgctccaacc cgaacaggtt cagcgacgac gggtcggctc
     1081 cgggtgggtg gcgatcgaag ccgccgaccg aggtcgtttc gatcatcacc cgccgtccgt
     1141 cgagatcgag cgcgagcagc aggtgaccgg gacgctcgat gaccacgacg cgatagccga
     1201 gctcacgggc caccaggccg tagagcagtg tggcggaaag acaattgaac tcgccgcgat
     1261 cgaacagccg cgtcaggctg tcggcgtcgc gacggtactg cgcgaggtag cggcggtgca
     1321 gcaggcggtg caggcgtcgg gccctcacgt agctcgcggg gccgggcccc atgcgcccga
     1381 tgatccgcgc gagctccgtt tgaacggcgg cgacgtcggc atgcagcgct cccggcgtga
     1441 tccgcgcttc ggtcgcggcc accgcctcgc cgacgagcgc ggcgagaccg tgtggcggga
     1501 acggaccgtc ggcggcggtg ggcgtgacgg gccacagcag caacgcaaca ccgagcatgc
     1561 aggaggagga catggtgaga ttccgtcgga ggatgctggt gggacccggg cggtcgacga
     1621 cctggttctg cttcactcga tctgcgcagc agaatctagg cccggctgtc gggtcgggca
     1681 agacgagaag cggtcccgcg gctgcccgct ttcgtaaccg gcccgccgcg gaccgtcagt
     1741 cgaaatcggg gctcaagcct cgggtcccgg cggctggatg cggtagccga tcccgcgtac
     1801 cgtgtcgagc cagttcgcgg cgttgccgag cttgttgcgc aaccgacgga tgtgcgtatc
     1861 gaccgtgcgc gacatcacct ccgagtcgta gccccagacc tccgacagca acgcctcgcg
     1921 cgaacggact cggcccgagg cgcggacgag ctcggccagc aggcgaaact cggtcagcgt
     1981 cagcttgatc gggcggccct cgacccgcgc ttcgtgcatc tgcatgtcga tctcgatcgg
     2041 accggcggtg atcagctcct cggggcgcgg cccggcctcc gcgctgcgtt ggagcaccgc
     2101 cttgacgcgc agcatcagct cgcgaggact gaacggcttg gtcacgtagt cgtcggcgcc
     2161 gaactcgaac gccagcaccc gatcgacctc ctcggtccgc gcgctgacga cgatcacccg
     2221 cgcgccggcg gtcgcctcat cgcgcttcag catcttgagc agctcgagcc cgctcatgtc
     2281 cggcagcatc aggtccagga tgacgagatc gacgttgtcg cgccggatgg cggccaacgc
     2341 gccctgcgcg gtcgtctccg cggtcgcccg aaagccgtcc ttggtcagcg cgccgcggag
     2401 aaactccgcg atatcgggtt cgtcctcgac gacgacgacc cgtgccgact gggtcttcgc
     2461 gttcatcatc ggtatctatc ggaacatccg cttgttcggt ggatgttaag aaatcgcaag
     2521 aaaccggtaa cattcgacgc acgatcgtca cgccggcccg aatccgcgat cccggggccc
     2581 gtcaccgtcg gctgcgcgcg atccgctcga cctgctcgag gtagttggcg acgtcgtccc
     2641 tcggctcgag ctcctgtgcc cgcttcaaca aggggaccgc cgcctcgtat cgcgacagct
     2701 cgaccagcag ctgcgcgtgg cggaccttgg cctcggcctc gaactcttcg atgctctcgg
     2761 cccgctcgta gtagaagatc gcccgttcgg gctcctgctg cttcgcgtag tgctgtccca
     2821 gcaggatcaa cgcgtcgccg tcgagcgggt cgatcgcgac gatctcttcc agtacgccga
     2881 ccgacgcccc gccctcgcca tcggcgacgg ccacccgcgc ctcgagcttg agcagcttct
     2941 tcttgtcgtc ggcctcgatg cgatcaccga acacccgctt gacctcggac agcaaggctt
     3001 tcgcctgatc gagcgctccg cgttgagcca gcacctcgac ccgctgcatc ggctgcgtca
     3061 ccggctgctg cgggttgccg gcgaccgcca gcccgtaagc ccgcaacgcg agatcccaca
     3121 agccctcgtt gacgtagatg tcacccagca tgtacaggct gggcaccgtc gccttgccca
     3181 tgcgctggac gatctcgaag ttctcggccg cggcgagcgg ccggccgaga ccgatgtagg
     3241 cgttggcctg gaggatccaa tagtcggccc gctccgggta ggtctcgatc agctcttcgc
     3301 acagcgtgac cgcctcgccg aacttctgct gcttgaagac gctttgcgtc agacccagct
     3361 tccagtcgag cgtttcgggc tgcagcagga ccgcgttgcg gtaggcggtc tcggccgaga
     3421 cgaactgccc ggtcgagctg taggcgtaac ccagcaaccc gaaggtcaaa ccgtcacgcc
     3481 cgcccagctc gatgacctcg ctcaacgagt cgacggcctt gtcgaaatcc ccctggcgga
     3541 cgcggatcag gccgaggttc ttgtgcgcgc ggcggaagct ggggaacttg gcgatcgcgt
     3601 tctcgtagct cgccgcggcc cgctcgagct cctcgcgctg gaaatagaga ttgcccaggg
     3661 tgaaatcgaa gatcgcgctc gtcgtgccgt cgaagtccgg atcccgtttc ttcttggtgt
     3721 tggacgcggg tggtgcgagg taccgctcga gctcctcgat cgcgtcggtg tcgctgccgc
     3781 cgctcatcag gcgcaggatc tcctccatct tctcgcgctc gaccgaggtg acccgcggct
     3841 cgagctcggc ctggaaaccg taggagccga ggaactcctt ctgaaaggtc gggtcggtcc
     3901 aacgctcgag cgcgacggga ttcgacccgt cgtcaccggt cccctcgccg gacggaagac
     3961 cggccaggcc gcaggtgacc gcgagcaggg gcagcagtgc gaacgggatc gtgcgtctca
     4021 tcggtcgaag tccttccccc agcccgcggg gctcagcccg cgttgaacgt gatcggcacg
     4081 cgcatcttga actggacctt ctcgcctttg cgcgtgcccg gctcgaatcg ccactgtctg
     4141 accgcctcca acgccgggcg ctcgaaggcg ccgtccgacg ccgtctcgat cgtcggggag
     4201 gtcacccgac cgtcggtgtc gacgacgaac acgacgtaga ccgtgccctg ccgcttgctg
     4261 cggcgcagct ccggcgggta ctgcggcatg cgctggaaga tgacccgcgg tcgctggtcg
     4321 agctcgccca gggagaagac gcgatcgagc tcctcgtcgc cgccctgggc catccgatcg
     4381 aacagctcga cggtgaagtc gccgaacacc ccgtcgccga gacccgggtt cagcgccagg
     4441 tcgagctggg ccagatcgag cggctgagcc ggttcgttga gcttcggcgg ctcgtccggc
     4501 ggttcttcct cctcggtctc ctcttccggg agcggcggcg gcggcggcgg cagctcgacc
     4561 ccgacctcgg tcacggaaag cagatcgtcc gggatcggat cgtaaatcga ctgcatcagc
     4621 ggcagcacga gaaacaaggc cagcgtcgcc ccgaccgcca gacaaaaggc gccggcaccc
     4681 tggagcaacg agatcgaacg tcgcgccgac ccttgccgca tctcgacccg tctctccccg
     4741 ctaggcctgt ttgcgggtcg cgacgctgac cttcgtcgcg ccgcccagct tggcctcatc
     4801 gatgacccgc accatcaggc cggcctgcac cgagctgtcc gcctggacga tgacgggcag
     4861 gtcctccttc tgcagcatgc gctcgaccag cggttggaca cccgcgatgc cgatctcccg
     4921 accgccgtag accaccgcgc cgtcggcggt gagggcgatc aggatgctgt tcttctcgag
     4981 cttcaccgcc gaggcggcct gcggcttgtc gacctccacc ccggactcct cgacgaaggt
     5041 cgtcgtcacg atgaagaaga tcaggaggat gaagacggcg tcgagcagcg gcgaaatgtc
     5101 gatgcccgcg gcctcttcct cacgaccgga aaacgaacgg aagcgtccca tcggtcgaac
     5161 tcctcaggcg gcctcgacgt cgcgcgccgc gcgaagccgc cggtagaacc gctgtgtgca
     5221 gacggtctcg aggtgcgcga ggaacgcctc gtagcgatcc cgcatgcgcg tcaggtggaa
     5281 ctggaagaac aaccccggca gcgcgatcac gagtcccgtc tcggtggtga tcagcgcctc
     5341 ggagatcccg ccggcgatca ggtccatcgt cttgtcgcca cccgagccgg tggcgagcgc
     5401 ggcgaacgtc gcgagcatcc cggtgacggt tcccagcaag ccgaacaacg gggcggtgct
     5461 gaccgcccgg cgcatgaatc ggagatcgcg gtcgaacggc gccaactcgg tcgaatcgag
     5521 ctcggcgaaa cgaaccgcta cctccttcag gtcggtcgcg cccatcgcga act
//
LOCUS       DASPGN010000365         6093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1588547, whole genome shotgun sequence.
ACCESSION   DASPGN010000365 DASPGN010000000
VERSION     DASPGN010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1588547"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VD788_10465"
     CDS             <1..303
                     /locus_tag="VD788_10465"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXV76730.1"
                     /translation="NGPLEWIPGSCNLGHVDAQGRGERIARELVRRTPRRMALLEPGD
                     VAAFGPYTFHRSRPNRSTRPRRVLINGYAYPGANSRVYPGCGTGREIYVFKHARWV"
     gene            complement(304..1038)
                     /locus_tag="VD788_10470"
     CDS             complement(304..1038)
                     /locus_tag="VD788_10470"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXV76731.1"
                     /translation="MSASWCALCNTQHAPSDACPGELEVTEPERHGWRVLVHTGSRTE
                     VYGVLIGPTGGAWRARVLTFPRMLWSIPGGRGTIKFAGRTPQEAERKAVEFILAFCKG
                     RGYSVLGEPAVVESAQLRNEASGHPAKLQARQRFLKSLPIRYGRQSPQADGQTGDLSH
                     GGLFVMTARPLPTDSPIKLRIDLDGFSVPLSGRVAWSRSAAETGRPAGMGVQLTQAPP
                     IYRRYVDRLRQRQEESAADGGDESPA"
     gene            complement(1045..1206)
                     /locus_tag="VD788_10475"
     CDS             complement(1045..1206)
                     /locus_tag="VD788_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76732.1"
                     /translation="MIAASEASGNGLISGFQAETLLWVWCVVVLLYAIVRSFARAEPD
                     PLRPSGPID"
     gene            complement(1238..2065)
                     /locus_tag="VD788_10480"
     CDS             complement(1238..2065)
                     /locus_tag="VD788_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXV76733.1"
                     /translation="MRVPPADRTAWLDGVPTPTGQLAIPLSDPAVLVGHGLFETVALR
                     DGHPLELAAHLDRLEGSARALAMPHPPRDELERICRAAVSAEPSACGWLKVLLTGGGR
                     SIVFTGPMEPEEDGAPVRAVLLPWRRDRRGPLVGIKTTSYAANRLALDHARRRGADEG
                     LWLNGRGHLCEACFANLFLVERGRLFTPGRREGLLPGVVRATVIRVARAGGIAVHETR
                     VRTKRLARCDEAFLTSSLGGVRPLVMVDGRAIGGGVPGPLTRRLAELVGRTRRGHRC"
     gene            complement(2062..3378)
                     /locus_tag="VD788_10485"
     CDS             complement(2062..3378)
                     /locus_tag="VD788_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HXV76734.1"
                     /translation="MSDTASACCERSLELPCDLESFFARALDDQDSAVWLDGRGEFGG
                     GWVSGTMMGLCCRRRAGIAARTGWLERIDRLVRRRLRRGGTARTGVVVLLGYEALDDA
                     VAGSRVVVVEVDASLAALSDGSTLLSACAAAGAAAERRFERLARRSAVEPSRPAAIAG
                     PVRTSLPRAAYLAAVARVKRHVTAGDVYQANLCQRFDAPVDGDGFTLYRRLAAASPAP
                     RSAYLRAAGSELVSISPEVFLRFAPPDRVETHPIKGTRSRQSDPLADRRAALELVASP
                     KDRAELLMIVDLERNDLGRICRTGSIRVTQVAELRSFPAVHHLVARVEGRLRPGVGPQ
                     EMIRATFPGGSISGAPKIRARELLRAIEPVPRGFFTGSLIWFGDDGSVDSSILIRTLV
                     LSGGTVRLGAGGGIVADSDPEGEWEESNLKAAPIARALGFDPREAR"
     gene            complement(3375..3980)
                     /gene="lexA"
                     /locus_tag="VD788_10490"
     CDS             complement(3375..3980)
                     /gene="lexA"
                     /locus_tag="VD788_10490"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXV76735.1"
                     /translation="MALTRRQRQVLDVIRDFIDTHGYSPSLEEIGSELHLSSVATVHK
                     HVSHLVQKGFVKRAWNQNRSIELVHQAPAAASVQLRLAGRIAAGHPLEAVETDEAIAV
                     PADMVRDVDNAYVLRVQGDSMINEQIRDGDFVVVERRAAAQDGETIVALIEGADATLK
                     RLFREGPNVRLQPANPQVEPIVVPAERLRIQGVVVGVIRKY"
     gene            4155..>6093
                     /locus_tag="VD788_10495"
     CDS             4155..>6093
                     /locus_tag="VD788_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXV76736.1"
                     /translation="MDPRPTIIRLTAIALALAGAPCATPAQDSPIADELIRQEEIRQT
                     IEDALELYFSSDGDRMANILASVGSVADLGDDAVPFLSNELAQQDPRTFFFAAYSLGR
                     IRTEGAARALREAIETAESQQIGEWPMMRKAWACWGLGMTGDPEAVTLLDSGRHLSGL
                     IPVGRQFTVLESAAVHTAPLSLEALHRLLKTLDDEIDPRTAYRRNVLDAIRRLGDPAS
                     TEVLIDLVDNGPPALRDDAAHALGAIDTPEAKAVLLARLDLPDPRARRGVVLGLQIHP
                     PTGGLDPVFARLAVETDAVIRGVLYDIIVRAGGQATTERLLAQWGKPDPQDRAQLAAL
                     LPYLDRDKVQDVLAMAVRDRATSVAYNAAEAAARLGDDRAIALLIDVVRSAELDATSA
                     IQFLGELRVEEAGDVLAWRLIDVELRDKRYDSHRRDRVIGIGRALVDVGYGGRIDDLK
                     QATVGLEDPKLVGYLEQLTAELELLATNRDKTKRWIPMLASDDPRHRELAYAYFARAG
                     DPRAGDELIRAFGRVEPSEGVRILDALGGIDTAASRELIARVLTAPEFDDYRRAELRE
                     MAAWSARRLGMYDTLVAAVERRRGRDFLPLVYAAVLGGERALELLGRYRIERLDYIGE
                     DHGVELTKIDWMRIELSHGRSI"
BASE COUNT          933 a         2176 c         2127 g          857 t
ORIGIN      
        1 aacggaccgc tcgagtggat ccccgggagc tgcaacctgg ggcacgtcga cgcccaggga
       61 cgcggggagc ggatcgctcg cgagctcgtg cgacgcacgc cgcggcggat ggcgctgctc
      121 gagccgggcg acgtcgccgc cttcggtccg tataccttcc accgcagccg gccgaaccgc
      181 tcgacccgtc cgcggcgggt gttgatcaac ggctacgcct accccggtgc caactcgcgt
      241 gtctaccccg gctgcggcac cggccgcgag atctacgtct tcaagcacgc tcgctgggtc
      301 tgactacgcg ggcgactcgt cgccgccgtc ggccgccgac tcctcctgcc gttgccgcaa
      361 tcgatcgacg taccggcgat agatcggtgg agcctgcgtc aactggaccc ccatgccggc
      421 cggtcgtccg gtctccgccg ccgaacgcga ccaggcgaca cgaccgctga gcggcaccga
      481 gaagccgtcg agatcgatcc gcagcttgat cggcgaatcg gtcggcaacg gccgcgcggt
      541 cattacgaac agcccgccgt gtgacaggtc gccggtctgc ccgtcggctt gcggcgactg
      601 cctgccgtag cggattggca ggctcttaag gaagcgctgg cgcgcctgca gcttggccgg
      661 atgaccggag gcttcgttcc ggagctgggc gctctcgacg accgccggct cgcccagcac
      721 cgagtagccg cgacccttgc agaaggcgag gatgaactcg accgccttgc gctcggcctc
      781 ctgcggcgtg cgcccggcga acttgatcgt gccgcgcccg cccggaatgc tccacagcat
      841 ccgcggaaag gtcagcacgc gcgcgcgcca ggcaccgccg gtcggaccga tgagcacgcc
      901 gtagacctcg gtgcgcgacc cggtgtggac cagcacgcgc caaccgtgac gttcgggttc
      961 ggtgacctcg agctcgcccg ggcaggcgtc agacggcgcg tgctgggtgt tgcagagcgc
     1021 gcaccacgaa gcggacatcg gcgctcaatc gatcggtccg gacgggcgga gcgggtcggg
     1081 ctccgcgcgg gcgaacgatc ggacgatcgc gtacaacaac acgacgacac accagaccca
     1141 caacagcgtc tccgcctgga acccgctgat caaaccgttc ccgctcgcct cgctcgccgc
     1201 gatcatcgac gtgctcccgt gatcgacccg ctccactcta gcatcgatgc ccacgacgcg
     1261 tgcggccgac gagctcggcc aagcgccgcg tcagcggacc gggcacgccg ccgccgatcg
     1321 cacgaccgtc gaccatgacg agcggcctga ccccgccgag gctcgacgtg aggaacgcct
     1381 cgtcgcaacg cgcgaggcgc ttcgtcctca cgcgcgtttc gtgtaccgcg atcccgccgg
     1441 cgcgtgccac tcggatcacg gtcgcccgga cgacgcccgg cagcaggccc tcccgccgac
     1501 ccggcgtgaa cagcctcccg cgctcgacca ggaacaggtt ggcgaagcag gcctcgcaca
     1561 ggtgccctcg tccgttgagc cacagccctt cgtcggcccc gcgacgacgc gcgtggtcca
     1621 acgcgagccg attggcggcg tagctcgtcg tcttgatgcc gacgagcggg ccgcggcggt
     1681 cgcgccgcca agggagcagc acggcccgca cgggtgcgcc gtcctcttcg ggctccatcg
     1741 gtccggtgaa gacgatcgag cgaccacccc cggtcagcag caccttgagc cagccgcagg
     1801 ccgacggctc cgcggagacc gcggcgcgac agatccgctc gagctcgtcg cgcggcgggt
     1861 ggggcatggc gagcgcgcgg gccgagcctt ccagccgatc gaggtgcgcc gccagctcga
     1921 gcgggtggcc gtcccgcagc gccaccgttt cgaacaggcc gtggccgacg agcaccgcgg
     1981 gatcggagag cgggatcgcg agctgaccgg tgggcgtcgg cacgccgtcg agccacgccg
     2041 tgcggtcggc cggcggcacc ctcatcgggc ctcgcgcgga tcgaagccca gcgctcgggc
     2101 gatcggcgcc gccttcagat tcgattcctc ccactcgccc tccggatcgg agtcggcgac
     2161 gattccaccg ccggcaccga ggcggacggt acccccggac aggacgagcg ttcggatcag
     2221 gatgctcgag tcgacgctgc cgtcgtcgcc gaaccagatc aagctgccgg tgaaaaaacc
     2281 gcgggggacc ggttcgatcg cgcgcagcag ctcacgcgca cggatcttcg gcgcgccgct
     2341 gatcgacccc cccgggaagg tcgcgcggat catctcctgc ggtccgacac ccggccgcaa
     2401 ccgcccctcg acgcgcgcga cgaggtggtg caccgccgga aagctccgca gctcggccac
     2461 ctgcgtcacc cggatcgaac ccgtccgaca aatgcgcccg aggtcgttcc gttcgaggtc
     2521 gacgatcatc aacagctcgg cgcggtcctt cggcgaggcg acgagctcga gcgccgcccg
     2581 gcgatcggcg agcgggtcgg actggcgcga gcgcgtgccc ttgatcgggt gcgtctcgac
     2641 gcgatcgggg ggcgcaaaac gcaggaagac ctcgggcgag atcgaaacga gctccgagcc
     2701 cgccgcccgc aggtaggccg agcgcggagc cggggaggcg gcggccaatc tgcggtagag
     2761 ggtaaagccg tcaccgtcga ccggggcgtc gaaccgctgg cagaggttcg cctgatagac
     2821 gtcacccgcc gtgacgtggc gcttgacccg cgcgaccgcg gcgaggtagg cggcgcgcgg
     2881 caggctggtc cgcaccgggc cggcgatggc cgcgggcctc gacggttcca cggcggagcg
     2941 gcgcgcgagg cgttcgaacc ggcgctcggc cgcggcgccg gcggcggcgc acgccgacag
     3001 cagcgtgctc ccgtccgaga gcgcggcgag cgacgcgtcg acctcgacga cgacgactcg
     3061 gctgccggcg accgcgtcgt cgagcgcttc gtagccgagc agcacgacga caccggtgcg
     3121 tgcggtcccg ccacgtcgga gccgccgccg gacgagacgg tcgatgcgtt cgagccatcc
     3181 cgtgcgagcg gcgattccag cccgtcggcg acagcagagg cccatcatcg tgcccgagac
     3241 ccagccgccg ccgaactcgc ctcggccgtc gagccatacc gcgctgtcct gatcgtcgag
     3301 cgcgcgcgcg aagaacgatt cgaggtcgca cggcagctcg agcgagcgct cgcagcaagc
     3361 ggaggcggta tcgctcaata cttgcggatc acgccgacga cgacgccctg gatccgcagt
     3421 cgctcggcgg ggacgacgat cggctcgacc tgtggattcg ccggctggag tcggacgttg
     3481 gggccctcgc gaaacagacg tttgagcgtc gcgtcggcgc cctcgatcaa cgcgacgatg
     3541 gtctcgccgt cctgcgccgc cgcacgacgc tcgacgacga cgaagtcgcc gtcgcggatc
     3601 tgctcgttga tcatcgaatc gccctgcacg cggagcacgt aggcgttgtc gacgtcccgg
     3661 accatgtcgg cggggacggc gatcgcctcg tcggtttcga ccgcctcgag cggatggccg
     3721 gcggcgatcc gccccgcgag acggagctga accgacgcgg cggcgggcgc ttggtggacc
     3781 agctcgatcg agcggttctg gttccacgcg cgcttgacga accccttctg gacgagatgc
     3841 gagacgtgct tgtggacggt cgcgaccgag gagaggtgaa gctcggagcc gatctcctcg
     3901 aggctcggcg agtaaccgtg ggtatcgatg aaatcgcgga tgacgtcgag cacctgacgt
     3961 tgtcggcggg tcagtgccat gcgggaccct cctccgaggt gaatggtcgc actcagccta
     4021 ggcgaaacac ccacgaaagt caaccaccgc tcgccgcgcc gtgctggtgg caggaagcgg
     4081 tttttgcctc gtttcccgct ggccggtata ttgcccattt tccggacccg agcccgggag
     4141 cggagcgccc ccgaatggac cctcgcccca cgatcatccg cctgacggcg atcgcgctcg
     4201 ctctggccgg ggcgccgtgc gcgacaccgg cccaggattc tccgatcgcc gacgagctga
     4261 tccggcagga ggaaatccga cagacgatcg aggacgcgct cgagctctat ttctcgagcg
     4321 acggcgaccg gatggccaac atccttgctt ccgtcggctc cgtggccgac ctcggcgacg
     4381 acgccgtccc gttcctctct aacgagctcg cgcagcagga cccccgcacg ttcttttttg
     4441 ccgcctactc gctcggccgt atccgcaccg agggggccgc ccgcgcgttg cgtgaggcga
     4501 tcgagacggc cgagagccag cagatcggtg aatggccgat gatgcggaag gcctgggcct
     4561 gttgggggct cggtatgacg ggtgaccccg aagccgtgac cctgctcgat tccggccgac
     4621 atctgtccgg gctgatcccc gtcgggcgcc agttcaccgt gctcgagtcg gcggccgtcc
     4681 acaccgcgcc gctgagcctc gaggcactcc accggctgtt gaagacgctc gacgacgaga
     4741 tcgacccgcg gacggcctac cgaaggaacg tgctcgatgc gatccgccgg ctcggcgatc
     4801 cggcctcgac cgaggtcctg atcgacctcg tcgacaacgg gccgccggcg ctgcgggacg
     4861 atgcggccca cgcgctcggg gcgatcgaca cgccggaggc caaagcggtg ttgctcgccc
     4921 gtctcgacct cccggacccg cgagccaggc gcggggtcgt gctcggactg cagatccacc
     4981 ctccgaccgg cgggctcgac ccggtattcg cgcggctcgc cgtcgaaacg gacgccgtga
     5041 tccgtggcgt gctgtacgac atcatcgtcc gtgccggtgg ccaggccacg accgaacggc
     5101 tgctcgccca gtggggcaag cccgatcctc aagaccgcgc gcagctggcg gcgctcctgc
     5161 cctacctcga tcgggacaag gtgcaggacg tgctcgcgat ggcggtacgc gatcgcgcga
     5221 cgtcggtcgc ctacaacgcg gccgaggcgg cggctcgctt gggcgacgac cgcgcgatcg
     5281 cgctcttgat cgacgtcgtc cgatccgccg agctcgacgc gacgtcggcg atccagttcc
     5341 tcggcgagct acgcgtcgag gaggcgggcg acgtgctcgc ctggcggttg atcgatgtcg
     5401 agttgcgcga caagcgctac gactcccacc ggcgcgaccg ggtgatcggc atcggccgcg
     5461 cgctggtcga cgtcggctac ggcggccgga tcgacgatct gaaacaggcg accgtcggcc
     5521 tcgaggaccc gaagctcgtc ggctatctcg agcagctcac cgccgagctc gagctgctcg
     5581 cgacgaatcg ggacaagacg aagcgctgga tccccatgct cgcgtccgac gacccgcggc
     5641 atcgcgagtt ggcctacgcg tatttcgccc gcgcgggcga tccgcgcgcg ggagacgagc
     5701 tgattcgggc gttcggtcgt gtcgagccga gtgaaggcgt gcggatcttg gatgcgctcg
     5761 gcggcatcga caccgccgcg tcgcgcgagc tgatcgcgcg cgtgctgacc gctcccgaat
     5821 tcgacgacta ccgccgcgcg gagctgcgcg agatggctgc ctggagcgcg cggcggctcg
     5881 gcatgtacga cacgctcgtc gcggccgtcg agcggcgccg cgggcgcgac ttcctgccgt
     5941 tggtctacgc ggccgtgctc ggcggcgaac gggcgctcga gctactcggg cgctaccgta
     6001 tcgagcggct ggactacatc ggcgaggatc acggggtcga gttgacgaag atcgattgga
     6061 tgcggatcga gctgtcccac ggtcggtcga tcg
//
LOCUS       DASPGN010000366        10472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_886471, whole genome shotgun sequence.
ACCESSION   DASPGN010000366 DASPGN010000000
VERSION     DASPGN010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886471"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2210
                     /locus_tag="VD788_10500"
     CDS             <1..2210
                     /locus_tag="VD788_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV76737.1"
                     /translation="GAGVIVAVIDTGVDRTHPDLADHIWTNPGEVPGNGIDDDGNGYV
                     DDTWGWDFCFDSPPVAAAHGTQVAGQVAGDGTGGTVTGMAPDAELMSLGIDCDTPSRA
                     WEASDYAIAHGAHVITQSYSWWWTDQPNYEGFRRQTDVELAAGVIHANSAGNNGSSQA
                     TYPIPYNVSTPANCPAPWVHPDQTLAGGISSVLAVGDVNWSTDVIAASSSIGPSAWED
                     IQANTDPAFPHTNPPEYRDYPYENGAEMGLIKPDLSAYGTGTLSTCPGPFYCQFSGTS
                     SATPHVSGTIALMLEANPEATPEELAQALMTTAQHRGSPGKNNVYGAGLLQAFPAVLA
                     VESGVVYSAHAIDDASTGNGDGMFDPGEQVVMQITVESRTDAAIEGLEGILSSTTPGV
                     TIHDHRATYPDLAARGTATSFAPHYSLSVDPGACATVIAFDLELRYAGSVRRSTFELR
                     VGEAQPVTLLDDDFESDLGWTSDPVAATQGFWVRQDPIGVSDDQGRPANPEDDTSPSG
                     THCWVTGNGELTGRKDENNNDVDGGPVILLSPPFGRTHMLSLTLSYDRWYYDVDSGNR
                     LRVEVSNDGGESWALLENLIFDEGGWSTQTWDLLALLPPTDDMRLRFWAEDDFADDPV
                     EAAVDEVRLQGTWVDCQEWVPPTLDAPNPVGDTLRVAPDPGGHTVLTWQAPPVDEGHD
                     AATLYRIVRAPGPQGPWTEIGSATSTGWVDVDAFGSDDSFYYRVFAENSGGAE"
     gene            2992..3633
                     /locus_tag="VD788_10505"
     CDS             2992..3633
                     /locus_tag="VD788_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV76738.1"
                     /translation="MAIDDDSIDRLARRLAGPGVWSSPTLVVKTALPRALARSADPLE
                     ADPHVGLLHPMIRRFWSAAVERASGRDRRELLAESEAAHDSRLKLLRAIDRHGGRLLV
                     GTDAPNPFVAPGFSIHHELQFFVDTGLPRPRVLEIATAEAAAFLDRAGEFGVIAPGTR
                     GDLLVLDGDPRQDLAALERPVGVMAAGRWYDRRSLEAEIERTRPQYLEGRPPR"
     gene            3732..4583
                     /locus_tag="VD788_10510"
     CDS             3732..4583
                     /locus_tag="VD788_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXV76739.1"
                     /translation="MGRRYVPVTMGSPRFRTVDVGECIVTEAWFPPGVRLPHHVHERP
                     CFAVMLDGSFDLDFAGRSYPCLPASVSTEPAGERHANRIRNGGAHVVVVQPDPATSEF
                     VRPLRGLLEEIHHFSHTDITRMARRVTLELRRPDDLSSLAIESLALGMLVAARRVERP
                     DRSGHPVPRWLSRAREFVHANYRRSFTIRHLAREAGVHPVHLARVFRSHFRTSVGEYV
                     RGLRLDWAARELAASDESIAVIALEAGFADQSHFTRAFKQHRGLTPGRYRRTVDRPVP
                     RRPGPER"
     gene            complement(4585..5103)
                     /locus_tag="VD788_10515"
     CDS             complement(4585..5103)
                     /locus_tag="VD788_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76740.1"
                     /translation="MVRRSHAIAGLIVVLSVALPAEGNSSPVASATGGYRWTIAEDDI
                     FGIEVTNRHLNVSARKHTDGSSSGIFVYDQVVEGESFRFVVSVTCFEVYDGNRAKVGG
                     VVEMSNFPPLPAGVFAWFQAIDNGEGVDALPDRSTLLGFGDEAANEAFCNSPDPPRFG
                     PWDVIGNLQVQP"
     gene            complement(5233..6051)
                     /locus_tag="VD788_10520"
     CDS             complement(5233..6051)
                     /locus_tag="VD788_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76741.1"
                     /translation="MVREFAGDPRVETFVMSFPETRETLSTFWTNLYLRGTMVFDPDG
                     AFSLFAYSQPDTPGVAASRSFVIGPDQSIAFPFFGHDPTAVTNRIYQVLGQPKGDHDG
                     DGDVDSADLLALTVCFTGEGGYAEPGCLSVDLDDDADVDCHDWYRFRDAWTEGGEAPP
                     IDSCPQTVELVVGLDELVLEPIAGAVGYDVVHGDLLELRDRHGDYATALRGCLADDLV
                     DSVVSYTLEPAPGSGAWFLARGVTVDSAMTYDTGAESQVGDRDVAIEGSPLACR"
     gene            complement(6091..7539)
                     /locus_tag="VD788_10525"
     CDS             complement(6091..7539)
                     /locus_tag="VD788_10525"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76742.1"
                     /translation="MITRAPALWTGILVGTLLIPAAAASESPRLRHVRRYDSAIGKHI
                     LSAQIVLDHHALINTTNRVCLLDLSAPGTSCVSQINGLDSTTTTLGPDGYVYVNLARS
                     GFAILHLDDTTLALSLVGEVAETEVFFEKMAVRGNRLYVAAHSAGIRVYDITDPGSPV
                     LVGTLEQGFHDAWDIAVVGSTAYVADGAGGLKLVDLTDETAPRIVAGEDPRTSAGTSQ
                     AVLASEDHVYVAAGGAGVAVYDAGDLSSRRRYDTPVCAKNLARVGPYLAVADIGGLEL
                     LRVETDGELTSVAGESSKFLHSPAGVALRLWHGVAAWGPDRVLASDWSTLDLYQLVDP
                     SADDQPDIIASTQRLHFPIDGGTRLVTVRNAGSAPLQLSGFSSTHPEFTTQAEAATIQ
                     PDDSVDLLIEYSGDGPASGLILVTSNDPDESPLPIQVFGETQHLDIGEPAVPFVLESW
                     TRDHTTGQFAYGTFDLQAHAGQVVFFHVFAPS"
     gene            7669..8580
                     /locus_tag="VD788_10530"
     CDS             7669..8580
                     /locus_tag="VD788_10530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV76743.1"
                     /translation="MGEEREKRAMHEQNRRSWNAVTPVHNAHKRDQPAFLRRGGTTLW
                     PDELELLGDLAGLSVVHLQCNCGQDTLCLSRIAGSVVGVDIADAAVDFARELSRATGL
                     RATFVRSDLFDWFDSTPERYDVAFSSYGTIGWLYDVTRWARGVARVLKPGGRLVLLEF
                     HPLVWSLGARGLTGDSYFVEGAIRENNGVNDYVGDALAPSGYEAGDGGFRNPEPAYGF
                     QWTVAEIVQGIVDAGFLIEIFREYPYANGCELFDGMRRIERNRYVMPEGLPSVPLMLG
                     ISARSPAAAQRLGQHVPRKNSSGPRTL"
     gene            8630..10426
                     /locus_tag="VD788_10535"
     CDS             8630..10426
                     /locus_tag="VD788_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXV76744.1"
                     /translation="MSGRFVVLSLLVLIPSAAATAPTTPVDPRVDRVERGLRPVVWFE
                     GDPLWTLAERMEHYGTPGISVAVIEDGKIAWYRGWGVADRETGEPVTADTLFQAGSVS
                     KPVAALGAVRMAADGLLALDGNVNDTLESWKVPENEFTRESPVTLTGLLSHTAGLTVH
                     GFPGYAAGDPLPTIVQVLDGHPPANTAAVRVDSLPGSNWRYSGGGYTVAQLLMSDAAD
                     RPFEVLMRETVLEPLEMKRSTFENPLPPAKLAAAAAGILPDGSAVKGKRHVYPEMAAA
                     GLWTTAEDLARFGIEVQQALAGRGRVLSREAAELLVEPVTPGYGRGFGLWTREDGAYF
                     GHNGWDEGFCAQLTLSRDGRHGAALMINSNHPDLMTELVNAIAHEYGWPGYRAYVPRP
                     IDRGALKRYSGRFRYNSEQSFAVERKGKRLFMQYVGGDPEELLHIGDGRFMRRGRTAP
                     ITFTETNGVVEFHFLLPDGGRQSHPQLAQDAVMPRELRARGDYDRAVAAYRAILDADA
                     NDAAASEDYLNNTGLGLVDEGHHEVGTDLLRVAADLYPQSANTWDSLGYAWRVRGDDE
                     QALEYYRLALERDPGFPSAVAAVAELVGGQGD"
BASE COUNT         1665 a         3553 c         3614 g         1640 t
ORIGIN      
        1 ccggcgcggg ggtgatcgtc gccgtgatcg acaccggtgt cgatcggacc catcccgacc
       61 tggccgacca catctggacc aacccgggcg aggtcccggg caacgggatc gacgacgacg
      121 gcaacgggta cgtcgacgac acgtggggct gggacttctg ctttgactcg ccgcccgtcg
      181 cggcggccca cggcacgcag gtggcgggcc aggtcgcggg cgacggcacc ggcggaaccg
      241 tgaccggcat ggcaccggac gcggagctga tgtcgctcgg catcgactgc gacacaccgt
      301 cccgagcctg ggaggcgagc gactacgcca tcgcgcacgg cgcccacgtg atcacgcaat
      361 cctatagttg gtggtggacc gaccagccga actacgaggg ttttcgccgg cagaccgacg
      421 tcgagctggc ggcgggcgtg atccacgcga actcggcggg caacaacggc agctcgcagg
      481 cgacctatcc gattccgtac aacgtctcga cgccggcgaa ctgcccggcg ccgtgggtcc
      541 acccggatca aaccctcgcc ggcggcatct cgtcggtgct cgcggtgggc gacgtcaact
      601 ggtcgaccga cgtcatcgcc gcgtcgtcct cgatcggccc gtcggcctgg gaggacatcc
      661 aggccaacac cgatcccgcc ttcccgcaca ccaatccgcc cgagtaccgg gactacccgt
      721 atgaaaacgg cgcagagatg gggctgatca agccggatct gtcggcctac ggcacgggca
      781 cgctatcgac ctgcccgggc ccgttctact gccagttcag cgggacctcg tcggccacgc
      841 cccacgtctc gggtacgatc gcgctgatgc tcgaggccaa ccccgaggcc acgcccgagg
      901 agctggcgca ggcgttgatg acgaccgcac agcatcgcgg cagcccgggc aagaacaacg
      961 tctacggcgc gggcttgctg caggcctttc ctgccgtgct ggccgtcgag tccggcgtgg
     1021 tctactcggc ccacgccatc gacgacgcgt ccacgggcaa cggcgacgga atgttcgacc
     1081 cgggcgaaca ggtcgtgatg cagatcacgg tcgaaagccg cacggacgcg gcgatcgagg
     1141 ggctcgaggg gatcctctcc agcaccacgc ccggtgtcac gatccacgat caccgcgcga
     1201 cctaccccga cctcgccgca cggggtacgg cgacgagctt cgcgccccac tactcgctga
     1261 gcgtcgatcc cggcgcatgc gccacggtca tcgccttcga tctcgagctg cgctacgcgg
     1321 gctcggtacg ccgctcgacg tttgagcttc gcgtcggtga ggcgcagccg gtcactctgc
     1381 tcgacgacga cttcgagtcg gacctcggct ggacctccga tcccgtcgcc gcgacccagg
     1441 gcttctgggt ccggcaggac ccgatcggcg tatccgacga ccaaggacgc ccggcgaatc
     1501 ccgaggacga cacctccccg tcgggcacgc actgctgggt cacgggcaat ggcgagctca
     1561 ccggccgcaa ggacgagaac aacaacgacg tcgacggcgg cccggtgatc ctcctttctc
     1621 cgccgttcgg tcgcacgcac atgctgtcac tgacgctgag ctacgaccgc tggtactacg
     1681 acgtcgactc gggcaaccgg ttgcgcgtcg aggtgtcgaa cgacggcggg gaaagctggg
     1741 cactgctcga gaacctgatc ttcgacgagg gcggttggag cacccaaacg tgggacctgc
     1801 tcgcgctgct gccgccgacc gacgacatgc ggctgcgctt ctgggccgag gacgacttcg
     1861 ccgacgatcc cgtcgaggcc gccgtggacg aggtgcggtt gcaaggcacg tgggtcgact
     1921 gccaggagtg ggtgccgccg acgctcgatg ctccgaaccc ggtcggcgac acgctgcgcg
     1981 tcgcgcccga ccccggtggc cacaccgtgc tgacctggca ggcaccaccc gtcgacgagg
     2041 ggcacgacgc cgcgacgctg taccgcatcg tgcgcgcgcc gggtccgcag gggccgtgga
     2101 ccgagatcgg atcggcgacc tcgacaggct gggtcgacgt cgatgccttc ggttccgacg
     2161 attccttcta ctaccgcgtg ttcgccgaga acagcggggg agctgagtga cgccgggcca
     2221 gccggcgcgc ccgttggatc tcgttccaac cgcgggcggc ggctcggcat cttgtcggat
     2281 cgagggaggc cgcaccgatg acgcgagcgg cttggctcgg tctgacgctc atcgccctcg
     2341 gatcgaccct gtacttcacc ggcgtgctct cgccgtcgag accggagcat gtcgccgtgg
     2401 ggcatcacgc agacggcacg tgggccgtga ccggttccac ggtcctgacg atgaccgaac
     2461 acggagcact cgagaatcac acggtcgtcg tccagggcga ccggatcgtc gtggtggcac
     2521 cctctgctcg agtgaagctg ccggccggga cgacgcgcgt cgacgggtcg gggcgcttcg
     2581 tcatgccggg actcgccgac atgcacgtgc actacgggtt cgacggggat ggtgcgttgt
     2641 tcctggcgaa tggcgtgacc acggtgcgca acatgtccgg gctcccgttc ctcgcagaca
     2701 cgatcgacag gatccgcagc ggacaggcgg tcggaccgac gatctactcg gccggtccgc
     2761 tgatcgacgg acccgggtcg ttctggcccc acgccgtgat tgccgatacg ccggagcagg
     2821 tccgcgacgc cgtgcggcgg actgcggcgt ccgggttcct cgcggtcaaa ctctacgata
     2881 agctgtcccg cgacgggttc cgcgccggaa tcgagcaggc ccgggcggag gggctgccag
     2941 cggagtctcg tcccgcccgg ggattccacc ggcgttcgac cgaacggatg gatggcaatc
     3001 gacgacgact cgatcgatcg gctcgcgcgg cgactggcgg gccccggcgt gtggagcagc
     3061 ccgacactgg tggtgaagac cgcgctaccg cgagccctcg cccgctccgc ggatccgctg
     3121 gaagccgatc cgcacgtcgg cctgctccat ccgatgatcc gccgcttctg gtcggccgcc
     3181 gtcgagcggg cctcgggtcg agatcgccgg gagctactcg ccgagtccga ggcggcgcac
     3241 gactcgcggt tgaagctgct gcgtgccatc gaccgacacg gcggccgtct gctcgtgggg
     3301 accgacgctc ccaacccgtt cgtcgccccc ggcttctcga ttcatcatga gctgcagttc
     3361 ttcgtcgaca ccggtctgcc gcggccgcgg gtgctcgaga tcgcgaccgc ggaggctgcg
     3421 gcctttctcg accgggcggg cgagttcggc gtgatcgcac ccggcacgcg aggtgatctg
     3481 ctcgtgctgg acggcgaccc gcgccaggac ctcgccgcgc tcgagcgtcc tgtcggcgtg
     3541 atggcggccg gaagatggta cgaccgccgg tcgctcgagg ccgagatcga gcggactcgc
     3601 ccccagtatc tcgaaggacg cccccctcgg tagccgctgc agaatccccg cgcgccgacg
     3661 gcgtggtcca cgcccgcgct cgccggttgg cccatactct aaccgaggac gacgcgggtc
     3721 gtgggggacg gatgggcaga cgctacgttc cggtgacgat gggcagccca cgcttccgga
     3781 cggtcgacgt cggggaatgc atcgtgaccg aggcctggtt cccccccggc gtgcgactcc
     3841 cacaccacgt gcacgagcgg ccgtgcttcg ccgtgatgct cgacggctcg ttcgatctcg
     3901 acttcgccgg gcggtcctac ccgtgtctgc ccgcgtcggt gtcgaccgag ccggccggcg
     3961 aacgccatgc gaaccgaatc cgcaacggtg gcgcacacgt cgtggtggtc cagcccgacc
     4021 cggcaacgag cgagttcgtt cgcccgctgc ggggtctcct cgaagagatc catcatttct
     4081 cccataccga cattacccgg atggcacgac gggtgacgct cgaactgcga cgaccggacg
     4141 acctctcctc gctcgcgatc gagagcctgg cgctcggcat gctcgtcgcg gcgcgacgtg
     4201 tcgagcggcc cgaccgaagc ggacaccctg taccgcggtg gctgtcgcgc gcgcgggagt
     4261 tcgtacacgc caactaccgt cgatcgttca ccatccgcca cctggcccgc gaggccggtg
     4321 tccatccggt ccatctcgcg cgggtgttcc gttcccattt ccgaacctcg gtcggcgagt
     4381 acgttcgcgg cttgcgtctg gactgggccg cacgagagct ggccgcctcc gacgaatcga
     4441 tcgcggtcat cgcgctcgag gcgggcttcg ccgaccagag ccatttcacg cgagccttca
     4501 agcagcaccg cggcctgacg ccgggccgct accggaggac ggtcgaccgg cccgtcccgc
     4561 ggcgacccgg cccggagcgt tagcctacgg ctgcacctgc aggttgccga tcacgtccca
     4621 cggtccgaag cgtggcggat cgggactgtt gcagaacgcc tcgttcgcgg cctcgtcgcc
     4681 gaatccgagc agtgtcgaac ggtcgggcag cgcgtcgacg ccctcgccgt tgtcgatcgc
     4741 ctggaaccag gcgaacaccc cggcgggcag cggcgggaag ttgctcatct cgacaacccc
     4801 cccgaccttc gcccgattcc cgtcgtacac ctcgaaacag gtgaccgaca ccacgaagcg
     4861 gaacgactcg ccctcgacga cctgatcgta gacgaagatg ccgctcgagc tgccgtccgt
     4921 gtgcttgcgg gcactgacgt tgagatgtcg gttggtcacc tcgatcccga agatgtcgtc
     4981 ctcggcgatc gtccagcggt atccgccggt tgcgctggcc accggtgagc tgtttccctc
     5041 cgccggcaac gcgacggaca acacgacgat cagaccggcg attgcgtggc ttcgtcgaac
     5101 catggtgctt ctcccttcta cgcccgtgca tcgacgggtc gcggttctgg ccgaatgcga
     5161 tgcggaaagt gtgggccgag gagccgcgcc ggtcttgaag ccatgcaaga ggtttttgaa
     5221 tcgaacgaac attcagcggc aggccagcgg ggatccctcg atcgcgacgt cgcggtctcc
     5281 gacctgcgat tccgcgccgg tgtcgtaggt catggccgag tcgacggtca cgccacgcgc
     5341 gaggaaccaa gcaccggacc ccggcgcggg ctcgagcgtg taggagacca cgctgtcgac
     5401 gagatcgtcc gcgagacagc cgcggagggc ggtcgcgtag tcgccgtgtc ggtctcgaag
     5461 ctcgagcagg tccccgtgga cgacgtcgta acccacggca cccgcgatcg gctccagcac
     5521 cagctcatcc agcccgacga ccagctcgac ggtctgcggg cacgagtcga tcggcggtgc
     5581 ctccccgccc tcggtccaag cgtcgcgaaa gcggtaccaa tcatggcagt cgacatccgc
     5641 gtcgtcgtcg aggtcgacgg ataggcatcc cggctcggcg tagccaccct ctccggtaaa
     5701 gcaaaccgtc agcgcgagga ggtccgccga gtcgacgtcg ccgtccccgt cgtggtcgcc
     5761 cttcggctgg ccgagcacct ggtagatccg gttggtcacc gccgtcggat cgtggccaaa
     5821 gaaaggaaag gcgatcgact gatcggggcc gatcacgaac gagcggctgg ccgcgacccc
     5881 cggcgtgtcg ggctgcgaat aggcgaacag cgagaacgct ccatccgggt cgaataccat
     5941 cgtgccgcgt agatagagat tcgtccagaa ggtcgagagc gtctctcgcg tttccggaaa
     6001 gctcatcacg aacgtctcga ctctcggatc gccggcgaac tccctgacca ccgtgttctc
     6061 gaggtccgcg accgcggacg ggcaggcggg tcaactgggc gcgaacacgt ggaagaacac
     6121 gacttgcccg gcgtgagcct gtaggtcgaa ggtgccgtag gcgaactgcc cggtggtgtg
     6181 atcccgcgtc caggactcga gcacgaacgg aacggccggc tcaccgatat cgaggtgttg
     6241 cgtttcgccg aagacctgga tcggcagtgg gctctcgtcg gggtcgttcg atgtcaccag
     6301 gatcaacccg gaggcgggcc cgtcgccgga gtactcgatc agcaggtcga cggagtcgtc
     6361 gggctggatc gtggctgcct cggcttgggt ggtgaactcc gggtgggtgc tcgagaatcc
     6421 cgatagctga agcggtgccg agccggcgtt gcggacggtc acgagcctcg tcccaccgtc
     6481 gatcggaaag tggagacgtt gggtcgacgc gatgatgtcc ggttggtcgt ccgcggacgg
     6541 gtcgaccagc tggtagaggt cgagggtgga ccaatcggag gcgagtacgc gatccggtcc
     6601 ccaggctgcg acgccgtgcc acagtcgcag cgcaacgccg gccggagaat ggaggaactt
     6661 cgacgattcg ccggccacgc tcgtcagctc cccgtcggtc tcgacgcgca acagctcgag
     6721 ccctccgatg tccgccaccg cgagatacgg accgactcgg gcgaggttct tggcgcagac
     6781 gggcgtgtcg tagcgtcgcc ggctcgacag gtcgccggca tcgtagaccg cgacacccgc
     6841 tccccctgcc gccacgtaga cgtggtcctc gctcgccagc accgcttgtg acgttccggc
     6901 ggacgttcga gggtcctcgc ccgcgacgat ccgcggcgca gtctcgtcgg tgaggtcgac
     6961 gagcttgagt ccgcccgcgc catcggcgac gtaggcggtg ctgccgacca cggcgatgtc
     7021 ccaggcgtcg tgaaagccct gttcgagggt cccgaccagt accggcgagc ccggatcggt
     7081 gatgtcgtag actcggattc ccgccgagtg ggccgcgacg tagagccggt tgccgcggac
     7141 cgccatcttt tcgaagaaga cctcggtctc cgcgacctca ccgacgaggg acagcgcgag
     7201 ggtcgtatca tccaggtgca ggatggcgaa tccggatcgc gcgagattca catagacgta
     7261 gccgtcgggt ccgagcgtcg tcgtggtgga atcgagtccg ttgatctggc tgacgcaaga
     7321 ggtcccgggt gcactcaggt ccaacaggca aacgcggttg gtggtattga tcagcgcgtg
     7381 gtggtcgaga acgatctgcg cgctgaggat gtgcttcccg atcgcgctgt cgtaacgccg
     7441 cacgtgtcgg agacgcggcg actccgaggc ggctgctgcc gggatcaaca aggtcccgac
     7501 caggatgccg gtccagagag cgggcgcgcg cgtgatcatc ccctccgttc gactggccgt
     7561 acttggatgg aatcacaacc gctcgcgtcg cgtcaggggg cccagatgac ggattcggtc
     7621 catgatccgc ggcgggcgtg agcagcgggc atcgggcagg gaggcgggat gggagaagaa
     7681 cgagaaaagc gagcgatgca cgagcagaat cgacgctcgt ggaacgccgt cacgccggta
     7741 cacaacgccc acaaacggga ccagcccgcg tttctgcgac ggggcgggac gacgctgtgg
     7801 ccggacgagc tcgagctgct cggcgacctc gccggactga gcgtggtgca cctccagtgc
     7861 aactgcggtc aggacacgct ctgtctgtcg cgcatcgccg gctccgtcgt cggggtcgac
     7921 atcgcggatg ccgcggtcga tttcgcgcgg gagctgtcgc gcgcaacggg gctccgggcg
     7981 acgttcgtcc gcagcgatct cttcgactgg ttcgactcga ctcccgagcg ctacgacgtc
     8041 gcgttctcga gctacggcac catcggctgg ctctacgacg tgacccgctg ggcccgaggc
     8101 gtggcccggg tgctgaagcc gggcgggcgt ctggtcttgc tcgagttcca cccgctggtg
     8161 tggagcctgg gcgcgcgggg tctcaccggg gactcctact tcgtcgaggg tgcgatccgg
     8221 gagaacaacg gggtcaacga ctacgtcggc gacgcgctgg ctccgtcggg ctacgaggcc
     8281 ggcgacggcg gcttccgcaa ccccgagccc gcgtacggct tccagtggac cgtggcggag
     8341 atcgtgcagg gcatcgtcga cgcggggttt ctcatcgaga tcttccgcga gtatccgtac
     8401 gcgaatggct gcgagctgtt cgacgggatg cgccgcatcg agcgcaaccg ctacgtcatg
     8461 cccgagggct tgccgtccgt cccgctgatg ctcgggatct cggcgcggag cccggccgcg
     8521 gcccagcggc tcggccagca cgtcccacga aagaactcgt cgggcccgcg cacgttataa
     8581 cttgtcgagg ggctcgtcgg ccgaggttca acagattcgg aggtgtcgga tgagcggtcg
     8641 gttcgtcgtc ctgtctctgc tggtgcttat cccgtcggcc gcggccaccg cgcccacgac
     8701 gcccgtcgac ccgcgtgtcg accgcgtcga gcgcggtcta cgtccggtgg tgtggttcga
     8761 aggcgatccg ctgtggacgc tggccgagcg gatggagcac tacggcacac ccggcatctc
     8821 ggtggcggtg atcgaggacg ggaagatcgc gtggtaccgc ggctggggtg tggccgaccg
     8881 ggagaccggc gagccggtca ccgcggacac cctgttccag gccggctcgg tcagcaaacc
     8941 ggtcgcggcc ttgggggcag tgcggatggc ggccgacgga ttgctcgcgc tcgacggcaa
     9001 cgtcaacgat acgctcgaga gctggaaggt gcccgagaac gagttcacgc gcgagtcgcc
     9061 ggtgacgttg acgggcttgc tcagtcacac cgcgggtctg acggtccatg gcttcccggg
     9121 ctacgccgcc ggtgatccgc tgccgacgat cgtgcaggtg ctcgacgggc atccaccggc
     9181 caacaccgca gcggtccgcg tggacagctt gccgggctcg aattggcgct actccggtgg
     9241 aggctacacc gtcgcccagt tgctgatgtc cgacgcggcc gatcgaccgt tcgaggtatt
     9301 gatgcgggag accgtgctcg agccgctcga gatgaagcgc agcaccttcg aaaacccgtt
     9361 accgccggcc aagctcgccg ccgccgccgc gggcatcctg cccgacggtt cggccgtcaa
     9421 gggcaaacgt cacgtctacc cggagatggc cgccgccggt ctgtggacga ccgccgagga
     9481 tctggcccga ttcggcatcg aggttcagca ggcgctcgcc gggcgtggtc gcgtgctgtc
     9541 gcgcgaggcg gccgagctgc tggtcgagcc ggtcactccg ggttacgggc gcggcttcgg
     9601 tctgtggacg cgggaggacg gggcgtactt cggccacaac ggttgggacg agggcttctg
     9661 cgcgcagctc acgctcagcc gggacgggcg ccacggcgcg gcgctgatga tcaactcgaa
     9721 ccacccggac ctgatgacgg agctggtcaa cgcgattgcc cacgagtacg gctggcccgg
     9781 ttaccgcgcc tacgtcccgc gaccgatcga ccgcggcgcg ctcaaacgct acagcggccg
     9841 gttccgctac aactccgagc agtcgttcgc cgtcgaacgc aaaggcaaac ggctgttcat
     9901 gcagtacgtc ggtggtgatc ccgaggaact gctccacatc ggcgacggcc ggttcatgcg
     9961 acgcgggcgg accgcgccga tcacgttcac cgagacgaac ggcgtcgtcg agttccattt
    10021 cctgctgccg gacggcggcc ggcagtcgca cccgcagctc gcccaggacg cggtcatgcc
    10081 gcgcgagttg cgggcgcgag gagactacga ccgggcggtc gcggcctacc gggcgattct
    10141 cgacgccgac gcgaacgacg ccgcggcgtc cgaagactac ctcaacaaca cgggcctcgg
    10201 gctggtcgac gagggccatc acgaggtcgg gaccgacctg cttcgcgtcg ccgccgatct
    10261 gtacccgcag tccgcgaaca cctgggacag tctgggctac gcctggcgcg tccgagggga
    10321 tgacgagcag gcgctcgagt actaccgact ggcgctcgag cgcgacccgg gcttcccgtc
    10381 ggcggtggcg gccgtcgcgg agctcgtcgg cgggcaaggc gattgacgaa tcagaaggtc
    10441 cagcgtccgg cgaacctcag cacctgatcc tg
//
LOCUS       DASPGN010000367         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_886576, whole genome shotgun sequence.
ACCESSION   DASPGN010000367 DASPGN010000000
VERSION     DASPGN010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886576"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="VD788_10540"
     CDS             complement(<1..1376)
                     /locus_tag="VD788_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HXV76745.1"
                     /translation="MGIQQTKLASRPGPLSALAVLALVAAGAVGADEPCRRLPRGIVG
                     WWPGEASADDRTLAAQHGLVVNGVAFGAGVIGEAFELDGVNDRIDVPDAPHLRPERFT
                     LAAWVRVDVVTEWACILCKQYGGGNADSLSLWLNFGVLQGGMFGFGEAIAPAPFPVGR
                     IVHAAVTYDGANIRLYQDGELVAAVNGPASAVPYDSNQLIIGAEDNGAGAYTAHFSGM
                     IDEPQIFGRALSSCEIRSLLAARTGGHCAGDSDGDLLPDFQDNCPGLSNAGQIDGDAD
                     GAGDACDCSPADPGVHSLDETFELEFTGDSLPDWCGDPSFTGNDTQYDVIRGDLADLP
                     VTGGGAVCRSACAAPLPGMLGWWTGDGGGTDLAGGHDGTLENGAGHSPGWTRDAFSLD
                     GSNDRVRTGNVMLPEVFSVAVWVNSARANQTAYRRIVETSFSTHFALTTDAGGTGYKL
                     IVRSPSQP"
     gene            complement(1455..2786)
                     /locus_tag="VD788_10545"
     CDS             complement(1455..2786)
                     /locus_tag="VD788_10545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HXV76746.1"
                     /translation="MSRAGAGKLVLGVLLTIVVVRLLVFVVAVGERSLQMDLSAFYTA
                     GRAAAAGLSPYVNHVGERPSLWDGVDTYRHSRFLYPPLVARAFRPLAALPYHAAKYVW
                     LALSLGALAGALALAARLARAARPSERPLALAVAAAAFHPLFTLLERGQSDSLTLLMM
                     MAAVWWMRNRSTSLAAGVLLALATLLKLHCVFIVPFLLVRRRWRVLAGFSAGGLLLLI
                     ASLVTDGAGGVAGYLTEELPRISRYGERGTAEMRLPPAVGVPLQRMLATGRTVMDGRS
                     YDVELFAFTINASAVRTPVGHAVWTGLRRLAPALAPAQVSLVFLLAGWLLLAGWQLRF
                     GEPDRTDTRAELVYWQCVLVVILLSAPTTWVMGTVWLLPIGAIVLGERDLRGARLVAA
                     GVCVCGLLGAGLPDPPGALADYRLVVAESVCLAGLLGRWAADSTPTSRDTP"
     gene            complement(2783..>3174)
                     /locus_tag="VD788_10550"
     CDS             complement(2783..>3174)
                     /locus_tag="VD788_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76747.1"
                     /translation="AEAELRAALAEQHQSKYHVELGKVLARQNRDREAIAAYLAAERL
                     EPRDYRIPHALGLLHRRTGDLASSAAALGRALRLEPRHTPSAVRLGEVLLESGRATEA
                     AEAFRYALQNDPDNREAREGLMRAERP"
BASE COUNT          505 a         1139 c         1144 g          386 t
ORIGIN      
        1 tacggctgcg acggcgacct gacgatcagc ttgtaaccgg ttccgccggc gtccgtcgtc
       61 agtgcgaagt gggtcgagaa agaggtctcc acgatgcggc ggtaggcggt ctggttcgct
      121 cgggccgagt tgacccagac cgccacgctg aacacctccg gcagcatgac gttcccggtg
      181 cgaacgcggt cgttcgaccc gtcgaggctg aacgcgtcgc gggtccagcc cggcgaatgg
      241 cctgctccgt tctccagggt gccgtcgtgg ccgccggcga ggtcggtccc gccgccgtcg
      301 ccggtccacc agccgagcat ccccggcagc ggcgcggcgc aggccgaccg gcacaccgcg
      361 ccacccccgg tgacgggaag atcggccagg tcgccgcgga tcacgtcgta ctgcgtgtcg
      421 tttccggtga acgatgggtc gccacaccag tcgggcaggc tgtccccggt gaactccagc
      481 tcgaaggtct cgtcgaggct gtggacgccc ggatcggcgg gggagcagtc gcacgcatcg
      541 cccgcgccgt cggcgtcccc gtcgatctgt ccagcgttgg ataggccggg gcagttgtct
      601 tggaaatcgg ggagcaggtc gccgtccgag tcgccggcgc agtgaccgcc ggtgcgggcc
      661 gcgagcaagg agcggatctc gcagctcgac agcgctcggc cgaagatctg gggctcgtcg
      721 atcatcccgg agaaatgggc ggtgtaggcg cctgcgccgt tgtcctcggc gccgatgatc
      781 agctggttcg agtcgtacgg aaccgccgag gccggcccgt tcaccgcggc gaccagctcg
      841 ccgtcctgat acaggcggat gttcgccccg tcgtacgtga ccgccgcgtg cacgatccgc
      901 ccgacgggga acggcgcggg ggcgatggcc tcgccgaacc cgaacatgcc cccctgcaac
      961 acgccgaagt tgagccacaa gctgagagag tcggcgttgc cgcccccgta ctgcttgcag
     1021 aggatgcacg cccactcggt gacgacgtcg acgcggaccc acgcggccag cgtgaaccgt
     1081 tccggccgca ggtggggtgc gtcgggcacg tcgatccgat cgttgacgcc gtcgagctcg
     1141 aacgcctcgc cgatcactcc ggcgccgaag gcgaccccgt tcacgacaag tccgtgctgg
     1201 gcggcgagcg tccggtcgtc ggcactggcc tcgcccggcc accaaccgac gatgccgcgc
     1261 gggaggcgtc gacagggctc gtcggccccg accgcacccg ccgcgacgag cgccagcacc
     1321 gccagcgccg agagcggccc cggccggctc gcgagtttcg tctgctggat tcccatgatg
     1381 cctcctcgtt ctcctgcggg gtgcgccgca ggacagcccg catcccacca tgcgagaagg
     1441 cccgcgaaga tccgtcacgg agtgtcgcgc gaggtggggg tcgagtccgc cgcccaccga
     1501 ccgagcaggc cggccaggca gaccgactcg gcgacgacga gccggtagtc cgcaagagcg
     1561 cccggcgggt cgggcagacc ggcgccgagc aggccgcaga cgcaaactcc ggccgcgacg
     1621 agccgcgccc cgcgcaggtc gcgctcgccg agcacgatcg cgccgatcgg tagcagccac
     1681 accgtcccca tcacccacgt cgtcggcgcc gacagcagga tcacgaccag cacgcattgc
     1741 cagtagacca gctccgcgcg cgtgtcggtc cgatcgggct cgccgaagcg gagctgccag
     1801 ccggcgagca acagccagcc cgccaggaga aacacgaggg acacctgtgc gggagcgagc
     1861 gcgggagcga gccggcggag acccgtccag accgcgtggc cgaccggcgt gcggacggcg
     1921 gacgcgttga tcgtgaaggc gaagagctcg acgtcgtacg agcggccgtc catcacggtt
     1981 cggcccgtcg ccagcatgcg ttgcagcggg acaccgaccg caggaggcag acgcatctcg
     2041 gccgtacccc gttcgccgta tcgtgagatg cgcggaagct cctcggtcag gtaaccggcg
     2101 acgccgccgg cgccgtcggt caccaggctc gcgatcagca gcagcagccc gccggcggaa
     2161 aaaccggcga gcacgcgcca cctgcgccgc acgagcagga acgggacgat gaacacgcag
     2221 tgcagcttca acagcgtggc caacgcaagc agcaccccgg cggcgagcga ggtcgagcgg
     2281 tttcgcatcc accacaccgc cgccatcatc atcagcagcg tcagcgagtc gctctgcccg
     2341 cgctcgagca gggtgaacag cggatggaac gccgcggcgg cgacggccag cgcgagcggc
     2401 cgctcgctcg gccgtgcggc gcgggccaga cgggcggcga gggccagcgc gccggcgagc
     2461 gccccgagcg acagcgccag ccaaacgtac ttggccgcgt ggtacggcag cgcggccaac
     2521 ggtcggaagg cgcgggcgac gagcggcggg tagaggaagc gcgagtgacg gtaggtgtcg
     2581 acgccgtccc acaacgacgg ccgctcgccg acgtggttga catagggcga caggccggcc
     2641 gcggcggcgc gacccgcggt gtagaacgcg gacaagtcca tctgcagcga tcgttcgccg
     2701 acggccacga cgaagacgag cagtcggacc acgacgatcg tcagcaggac gccgagcacg
     2761 agcttgcccg cgccggcccg gctcacgggc gctcggcgcg catcagcccc tcgcgggcct
     2821 cgcgattgtc ggggtcgttc tgcagggcgt agcgaaacgc ctcggccgcc tcggtcgcgc
     2881 gccccgactc gagcagcacc tcgccgagac gcacggcgct cggcgtgtgg cgcggttcga
     2941 gccgcagcgc gcggcccagc gccgcggccg aggatgccag atcgccggtc cgccggtgca
     3001 gcagaccgag cgcgtgcggg atgcggtagt cacgcggctc gagccgctcg gcggcgaggt
     3061 aggccgcgat cgcctcccgg tcgcggttct ggcgagcgag caccttgccc aactcgacgt
     3121 ggtacttcga ctgatgctgc tcggcgagcg ccgcgcgcag ctcggcctcg gcct
//
LOCUS       DASPGN010000368         8695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_769286, whole genome shotgun sequence.
ACCESSION   DASPGN010000368 DASPGN010000000
VERSION     DASPGN010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769286"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VD788_10555"
     CDS             complement(<1..614)
                     /locus_tag="VD788_10555"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HXV76748.1"
                     /translation="MTGLLAIGLANALTALVAALVVSIVARFLERPALVHAMWLLVLL
                     ELLAPPVFEIDMLPGALRAVPSVATFVVADAGAPTGAETPAGARATVAPERVATPPAG
                     VETDLLGWAIAAWLAGAAVVIGLALVRVVRLHRRLRGPARAPRRLRRRLDRLAREMGL
                     GRPPALRIVRSHLSPCVWLRPGGSELLFPAALLGRLTPRETDALL"
     gene            complement(611..1006)
                     /locus_tag="VD788_10560"
     CDS             complement(611..1006)
                     /locus_tag="VD788_10560"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXV76749.1"
                     /translation="MPRGRRGVTDAELDVLKVLWDRGPSTIRELTDRLYPDGQTSHYA
                     TVQKLLERLQAKRCVARRSAGRAHLFRATVDREELIEDGLRRTAERLCEGSLTPLLTH
                     LVRASRLTEDEAARLRRLVDDLDRKRGGR"
     gene            complement(1081..2538)
                     /locus_tag="VD788_10565"
     CDS             complement(1081..2538)
                     /locus_tag="VD788_10565"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXV76750.1"
                     /translation="MSRQSTILVAMLAAALGCATCVFGARESEEIRIAAAALSSEAPE
                     ERLRAVCALADAGDRAAAAIPALVAVLGDERAVESTRCQRDGPTIDEERWFTRTSPGR
                     EAARALAALGEPALEALIERLGATDAVERRNAVWALGEIEDRAVVPRVVAALGDPDAT
                     VRREAVVALGKIGQFETRAAVTPLLGDPHAGVRAAAVRALVELGDPRAAGPLTAALDD
                     PDAAVREAAAEALGEVDDPGAADRLAARVRDPDEEVRRAAVEALSELRPDGAFKALAG
                     AIDDPSREVREAVAEALGETRSTHAASTLGALLGDSHWSVRESAASGLGELGDARGVE
                     PLIGVLDDPNWRVREAVCESLGELQHRRAVTALVGRLADPSWRVRTAAVEALGELGDE
                     RALDGLSQAIADDHHEVRVAAAEALGRFDDTRARTALERGLDDRQPAVRVAVLDALAE
                     SGDPALVDRIEPLLADPHPAVRETAVDALAELAGD"
     gene            2678..3049
                     /locus_tag="VD788_10570"
     CDS             2678..3049
                     /locus_tag="VD788_10570"
                     /inference="COORDINATES: protein motif:HMM:NF040913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SaoD/DsrE family protein"
                     /protein_id="HXV76751.1"
                     /translation="MKVAYVFSTSGHTASYKLGQMILPQMERREHGVEVVGMMFFDDN
                     TFLLRRGDPMGERLSRVAREQNVLLMMCDSCALERGLAEGGRWPEKSTAKDTVAGVVT
                     GCFPDLYRALAANPPDQVITL"
     gene            complement(3059..4735)
                     /gene="ettA"
                     /locus_tag="VD788_10575"
     CDS             complement(3059..4735)
                     /gene="ettA"
                     /locus_tag="VD788_10575"
                     /inference="COORDINATES: protein motif:HMM:NF008775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HXV76752.1"
                     /translation="MPEFIYTMKDLKKVVPPKREILKGIWLSFFHGAKIGVLGLNGAG
                     KSSLMRIMAGVDREFDGEAFPAKGIKIGHLPQEPELDESETVLGNVRQGVAETMGLLE
                     RFNAVSDQLAEPLDAEQMARLLDEQAKLQDRIDAAGAWELESRLELAMGALRCPPADA
                     RVENLSGGERRRVALCRLLLQRPDLLLLDEPTNHLDAESVLWLEHHLRDYPGTIVAIT
                     HDRYFLDNVAGWILELDRGAGIPWEGNYSSWLEQKQQRLTREEKASAARARTLERELD
                     WVRMAPRARQAKSKARLAAYEKLLAAETERQTELEQIYIPAGPRLGDVVIEADRLTKG
                     YDDRLLIDELSFKLPPGGIVGVIGPNGAGKTTLFRMITGQEQADSGTLRVGETVRLAY
                     VDQKRDVLNPEHTVWQAISEGNDQIELGSRRVPSRGYVSSFNFRGADQQKRVGSLSGG
                     ERNRVHLARVLKSGANVLLLDEPTNDLDVDTLRALEEALLAFAGCVVVISHDRWFLDR
                     IATHILAFEGDSRVEWFEGNFDDYEADKKRRLGPDADIPRRIQYRKLTRD"
     gene            complement(4837..5115)
                     /locus_tag="VD788_10580"
     CDS             complement(4837..5115)
                     /locus_tag="VD788_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXV76753.1"
                     /translation="MGTRLFVGNLPFTADEDEVRSLFEQNDRTVSEVKLITDRDTGRP
                     RGFGFVEMGSTEDADQAIRELNGYELNGRQLNVNEARERNGGGGGRRF"
     gene            5524..6768
                     /locus_tag="VD788_10585"
     CDS             5524..6768
                     /locus_tag="VD788_10585"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXV76754.1"
                     /translation="MSTIRSIPTRFADFALGASIQRAIVAAGFLEPRPIQLQTIPAAL
                     AGRDVLGLAQTGTGKTAAFALPLLERLLPRLSTRARGPRVLIVAPTRELATQIGEEID
                     RLARYTRVKTAVVFGGVSARPQIAALRERPAIVVACPGRLLDLLAQGELALGDVETLV
                     LDEADHMFDMGFLPDVRRILAALPAERQNLLFAATMPPEIRGLAQQLLRDPLVVELSH
                     SKPVDTIDHALYPVDSERKLEALQAILAEDGFVSAIVFTRTKHRARRLARQLDAAGHR
                     AVALQGNMSQNARERAMTGFRQRRFDVLVATDIAARGIDVEQVSHVVNFDMPGTPDAY
                     THRIGRTGRAERDGRAYTFVTTDDRAMVEQVERRIGQRIPRRSFDGARCLAAEPVRSA
                     PAVSRGGRAARAPRSSSRGRRR"
     gene            complement(6707..8035)
                     /locus_tag="VD788_10590"
     CDS             complement(6707..8035)
                     /locus_tag="VD788_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXV76755.1"
                     /translation="MSKWVSAMLVVLAPQLIAGETPADMAAAIERDYREHLADLFVEL
                     HRNPELSFREFRTAARLAEELRSAGIEVSEKVGGTGVVGMLSNGEGPLVMVRADMDGL
                     PIAESSGLPYASTARQVDITGAEVPVMHACGHDVHVTAMVGTARRLAALREHWSGTVM
                     FVGQPAEERIGGAKAMLADGLYSRFGVPDYALALHVSAGDPAGKISLEPGLIASSSDS
                     VDVTVRGIGAHGASPHQGRDPIYVAAQLVIALQGLVSRELSPLEPGVVTVGSIHGGFK
                     HNIIPDRVELQLTVRADGEATRVKLLDGIERIAEHVGRAAGLPDELLPVVVRSTESTP
                     ATVNDPALAARIRRAFVDRLGEQALHDDVREGMGAEDFAYFLGTPDRVPGCYFSVGGT
                     PQADLAAADAGGPPVPSHHSPFFKIEPEPSVTAGVHAMTIAALELLGRRD"
     gene            complement(8401..>8695)
                     /locus_tag="VD788_10595"
     CDS             complement(8401..>8695)
                     /locus_tag="VD788_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXV76756.1"
                     /translation="ILRTTEYGDPRRDREALEKLSPIHFVDRVEDPLLILHGATDPRV
                     PVGEALQFHQAVARRDVPAELIIFPDEGHGVQKRPNRVLSTGHVIRFFDEHLR"
BASE COUNT         1289 a         3148 c         2967 g         1291 t
ORIGIN      
        1 agcagtgcgt cggtctcacg cggcgtcaac cgtccgagca gcgcagccgg gaacaacagc
       61 tcggagcccc ccggccgcaa ccagacgcac ggtgagaggt gcgagcgaac gatgcgcagc
      121 gccggcggac gcccgaggcc catctcgcgc gcgagccggt cgagccggcg acgcaggcgg
      181 cgcggggcgc gggccgggcc gcggagccga cggtggagtc gaacgacgcg caccagcgcg
      241 agcccgatca cgacggcagc ccccgcgagc caggctgcga tcgcccagcc gagcaggtcg
      301 gtctcgacgc cggcgggcgg ggtcgcgacg cgctcgggcg caacggtggc tcgtgcaccg
      361 gccggcgtct cggccccggt gggcgcgccc gcgtcggcga cgacgaaggt cgcgacggac
      421 ggcacggccc gcagcgcgcc gggcaacatg tcgatctcga acaccggcgg cgccaacaac
      481 tcgagcagga cgaggagcca catcgcgtgg accagcgccg gtcgctcgag gaaacgcgcg
      541 acgatcgaca cgacgagcgc cgcgacgagc gccgtcagcg cgttggccag cccgatcgcg
      601 agcaagccgg tcaccgtccg ccccgcttgc ggtcgaggtc atcgaccagc cgccgcaaac
      661 gtgcggcctc gtcctccgtc agccgcgacg cgcggaccag gtgggtcaac agcggggtca
      721 gtgaaccctc gcacaggcgt tcggccgttc ggcgcagccc gtcctcgatc agctcttcgc
      781 ggtcgacggt cgcgcggaag agatgggcac gaccggcgga ccggcgcgcc acgcagcgtt
      841 tggcctggag ccgttcgagc agcttctgca ccgtcgcgta gtgcgaggtc tggccgtcgg
      901 gatagagccg gtcggtcagc tcgcggatcg tcgacgggcc gcgatcccac agcaccttca
      961 gcacatcgag ctcggcgtcg gtcacgccgc ggcggccacg cggcacggtc tcttctcctc
     1021 aggcgaattg tctgaccacc aagctaagac aactcgtctg ggttgtcaag cccggcgggg
     1081 tcagtctccc gccagctcgg ccagcgcgtc gacggcggtc tcgcggaccg ccgggtgcgg
     1141 atcggccagc agcggctcga tccggtcgac cagcgccgga tcgcccgact cggccagcgc
     1201 gtcgagcacc gccacccgaa cggcgggttg ccggtcgtcg aggccccgct cgagcgccgt
     1261 gcgcgcccgc gtgtcgtcga agcgacccaa cgcttcggcc gccgcgaccc gcacctcgtg
     1321 gtggtcgtcg gcgatcgcct ggctcaggcc gtcgagcgcg cgctcgtcgc ccagctcgcc
     1381 gagcgcctcg accgcagcag tccgtacccg ccacgacggg tcggccagcc ggccgaccag
     1441 ggccgtgacg gcccgcctgt gctgcagctc gccgagcgac tcgcagaccg cctcgcggac
     1501 gcgccagttc ggatcgtcga gcacgccgat caacggttcg acaccgcgag cgtctcccag
     1561 ctcccccagc ccgctcgcgg cgctctcgcg gaccgaccag tggctgtcgc cgagcagggc
     1621 gccgagggtg ctcgccgcgt gcgtcgaacg ggtctcaccg agagcctcgg ccaccgcctc
     1681 gcgcacctcg cgggacgggt cgtcgatcgc gccggccaac gccttgaacg cgccatccgg
     1741 gcgcagctcg gagagtgcct cgaccgccgc gcggcgcact tcctcgtccg gatcccgcac
     1801 ccgcgccgcc agacgatcgg cggcgccggg gtcgtcgacc tcgcccagcg cttcggccgc
     1861 ggcctcccgc accgcggcgt ccgggtcgtc gagcgccgcg gtcagcggac cggcggcgcg
     1921 cggatcaccg agctcgacga gcgctcggac cgccgccgcc cgcactccgg cgtgcgggtc
     1981 accgagcaac ggggtgaccg ccgcgcgcgt ttcgaactgt ccgatcttgc cgagcgcgac
     2041 gaccgcctcg cgccgcaccg tcgcgtcggg atcgccgagt gcggcgacga cgcgcggcac
     2101 cacggcccga tcctcgatct cgcccagcgc ccacacggcg tttcgccgct cgaccgcgtc
     2161 ggttgcgccc agccgctcga tcaacgcctc gagcgccggc tcgccgagcg ccgcgagagc
     2221 ccgcgccgct tcgcgccccg ggctggtccg ggtgaaccac cgttcctcgt cgatcgtcgg
     2281 gccgtcccgc tggcaccggg tcgactcgac ggcacgctcg tcgccgagca cggcgacgag
     2341 cgcggggatc gctgcggcag cgcggtcgcc cgcgtcggcc aacgcgcaga ccgcccgcag
     2401 gcgctcctcg ggcgcctcgc tcgacagcgc agccgcggcg atgcggatct cctcgctctc
     2461 acgtgcgccg aagacgcagg tcgcacagcc gagggcggcg gccagcatcg ccacgagaat
     2521 cgtcgactgt cgggacatcg gtgcctcctc cgggtcggtt gcatccgtta caacaccggc
     2581 ggccgggcca gggtttacgg gaacatcggc tagaatcgcc ccgtgccgtg gggtggccgc
     2641 cgtgcgcgag ccgccgcgag ccaccggagg agtcgagatg aaggtcgcct acgtattcag
     2701 cacgtcgggg cacacggcca gctacaagct ggggcagatg atcctgcccc agatggaacg
     2761 gcgcgagcac ggcgtcgagg tcgtcggcat gatgttcttc gacgacaaca ccttcctgct
     2821 gcgccgcggc gatccgatgg gcgagcggtt gtcgcgggtc gcgcgcgaac agaacgtctt
     2881 gctgatgatg tgcgattcgt gcgcgctcga gcgtgggttg gccgagggag gccgctggcc
     2941 ggagaaatcg accgcgaagg acacggtcgc cggcgtcgtg accggttgct tccccgatct
     3001 gtatcgcgca ctggccgcca acccgccgga ccaggtgatc acgctatagc tcggacgatc
     3061 agtcgcgcgt cagtttgcgg tactggatgc ggcgcgggat gtcggcgtcc gggcccaacc
     3121 ggcgcttctt gtcggcttcg tagtcgtcga aattgccctc gaaccactcg acgcgagagt
     3181 cgccctcgaa ggcgaggatg tgcgtcgcga tgcgatcgag gaaccagcgg tcgtgactga
     3241 tcacgacgac gcatccggcg aacgcgagca acgcctcctc gagcgcgcgc agcgtgtcga
     3301 cgtcgagatc gttggtcggc tcgtcgagca gcagaacgtt cgcaccgctc ttgaggaccc
     3361 gtgccaggtg cacgcggttg cgctcgccac ccgacagcga cccgacgcgc ttctgctggt
     3421 cggcgccgcg gaagttgaac gacgagacgt agccgcgtga cgggacgcga cgcgagccga
     3481 gctcgatctg gtcgtttcct tcggagatcg cctgccacac cgtgtgctcg ggattcaaca
     3541 cgtcgcgctt ctggtcgacg taggccaggc gaaccgtctc gccgacgcgc agcgtcccgg
     3601 agtccgcctg ctcctggccg gtgatcatgc ggaacagcgt cgtcttgccc gcgccgttcg
     3661 gcccgatcac gccgacgatc cctcccgggg gcagcttgaa cgacaactcg tcgatcagca
     3721 gtcgatcgtc gtagcccttg gtcagccgat cggcctcgat caccacgtcg cccaatcgcg
     3781 ggccggccgg gatgtagatc tgctcgagct cggtttgacg ctcggtctcc gcggcgagca
     3841 gcttttcgta cgccgcgaga cgcgccttgc tcttcgcctg ccgcgcccgc ggtgccatcc
     3901 gcacccagtc gagctcgcgc tcgagtgtcc gagcgcgcgc ggccgaggcc ttctcctcgc
     3961 gggtcagccg ttgctgcttc tgctcgagcc aggaggagta gttgccctcc cacgggatgc
     4021 ccgcgccgcg gtccagctcg aggatccagc ccgcgacgtt gtcgaggaag tagcggtcgt
     4081 gcgtgatcgc gacgatcgtt ccgggatagt cacgcaggtg gtgttcgagc cacagcaccg
     4141 actcggcgtc gaggtggttc gtcggctcgt cgagcagcag cagatccggc cgttgcagca
     4201 gcagccggca cagcgcgacg cgccgccgct ccccgcccga cagattctcg acacgcgcgt
     4261 cggccggcgg gcagcgcagg gcgcccattg ccagctcgag acggctctcg agctcccacg
     4321 cgccggccgc gtcgatccgg tcctggagct tggcctgctc gtcgagcagc cgcgccatct
     4381 gttccgcgtc gagcggctcg gcgagttgat cgctgaccgc gttgaaccgc tcgagcaacc
     4441 ccatcgtctc ggccacgcct tggcggacgt tgcccagcac cgtctcgctc tcgtcgagct
     4501 cgggttcctg cggcaggtgg ccgatcttga tgcccttggc cggaaaggcc tcgccgtcga
     4561 attcgcgatc gacgccggcc atgatacgca tcaggctgct cttgcccgca ccgttcagac
     4621 cgagcacgcc gatcttcgcg ccatggaaga aagaaagcca gatgcccttg aggatctcgc
     4681 gcttgggcgg gacgaccttc ttcaggtctt tcatcgtgta aatgaactcg ggcatgacgg
     4741 ccgggattct atccgattcg gtgcggttcg tccgctggac aaaaaaaggg cgcccgtcgc
     4801 gggcgccctc catcggcatg ttgaagttgc gcggtcctag aaccggcgcc cgccgccgcc
     4861 accgttgcgc tcgcgcgcct cgttgacgtt cagctgccga ccgttcaact cgtagccgtt
     4921 gagctcgcga atcgcctggt cggcatcttc ggtgctgccc atctcgacga aaccgaaccc
     4981 tcggggtcgg cccgtgtcgc ggtccgtgat cagcttgacc tccgacacgg tgcggtcgtt
     5041 ctgctcgaac agcgagcgaa cctcgtcctc gtcggcagta aaaggcagat taccgacaaa
     5101 caatctcgtc cccatctctc agactcctcg ctcccgctcg gggagcacga tctcaggtac
     5161 ccggcccatg actcgaacca atgagtcagg aactacgccg aagcgctacg caccttggcg
     5221 aacggtttcg gcacggcaaa agtcgtttct gtacgaatga ttgcgaccgg gaacctgtgg
     5281 ggcaaggtat aaggcggagc ggcggttgtc aaggaaccgg gtcccacccg cggggcccgg
     5341 cttgacaggg cggcccgcgt gctctacctt gcgaccggcc ggatcctccg gcccgcggcg
     5401 cacaacaggg tcttcccgga tcgcgccgcc agatcacccc ttacctactt ctgttgtgtg
     5461 ggaaggcagc ggcggccgtg gtgcgtcgag ttcgccccgg ggtccgccga ggaccgcaaa
     5521 ccgatgtcca cgattcgatc gatcccgacc cgttttgccg acttcgcgct gggtgcctcg
     5581 atccagcgcg cgatcgtcgc cgccggattc ctcgagccgc gtccgatcca gctccagacg
     5641 atccccgcgg cgctcgccgg acgcgacgtg ctcggcctgg cgcagaccgg aaccggcaag
     5701 accgcggcct tcgccttgcc actgctcgaa cggctgctcc cccgcctgtc gacgcgagcg
     5761 cgcgggccgc gcgtgctgat cgtcgcgccg acccgtgagc tcgccacgca gatcggcgag
     5821 gagatcgacc gactggcgcg gtacacgcgc gtcaagaccg cggtcgtgtt cggcggtgtc
     5881 tccgcacgcc cgcagatcgc ggcgttgcgc gagcggccgg cgatcgtcgt cgcctgtccc
     5941 gggcgtctgc tcgacctact ggcccagggc gagctcgccc tgggcgacgt cgaaacgctc
     6001 gtgctcgacg aggcggatca catgttcgac atgggcttcc tgcccgacgt gcgccggatc
     6061 ctcgccgcgc tgccggccga gcgccagaat ctgctgttcg ccgcgacgat gccacccgag
     6121 atccgcggcc tggcgcagca gctgctgcgc gacccgctgg tcgtcgagct gtcccattcg
     6181 aagccggtcg acacgatcga tcacgcactg tatccggtcg attccgaacg gaagctcgag
     6241 gcgctgcagg cgatcctcgc cgaggacggt ttcgtttcgg cgatcgtctt cacccgcacc
     6301 aagcaccgcg cgcgcaggct cgcgcggcag ctcgacgccg ccggccaccg cgcggtcgcg
     6361 ctgcagggga acatgtcgca gaacgcccgc gagcgcgcga tgaccggttt ccggcagcgc
     6421 cggttcgacg tgctggtcgc gaccgacatc gccgcccgcg gcatcgacgt cgagcaggtg
     6481 tcacacgtcg tcaacttcga catgcccggc actcccgacg cctataccca tcggatcggt
     6541 cgcaccgggc gcgccgagcg cgacggccgg gcctatacct tcgtcaccac cgacgatcgc
     6601 gcgatggtcg agcaggtcga acggcggatc ggccagcgca tcccgcgccg gtcgttcgat
     6661 ggcgcgcgat gtttggccgc ggagcccgtg cgcagcgcgc cggccgtcag tcgcggcggc
     6721 cgagcagctc gagcgccgcg atcgtcatcg cgtggacgcc ggcggtgacc gacggttcgg
     6781 gctcgatctt gaagaacggc gagtggtgcg acggaaccgg cggaccgccg gcgtcggctg
     6841 cggcgagatc ggcctgcggc gtaccgccga cgctgaagta gcaaccggga acacgatccg
     6901 gcgtgccgag gaagtaggcg aagtcctcgg cgcccattcc ctcgcgcacg tcgtcgtgca
     6961 gcgcctgctc gccgagccgg tcgacgaacg cccggcggat ccgcgcggcc agtgcgggat
     7021 cgttgaccgt tgccggcgtc gactccgtcg accgtacgac caccggcagt agctcgtcgg
     7081 gcaggccggc ggcgcgcccc acgtgctcgg cgatccgctc gattccgtcg agcagcttga
     7141 cccgcgtcgc ttcgccgtcg gcgcgcaccg tcagttgcag ctcgacccgg tccgggatga
     7201 tgttgtgctt gaagccgccg tggatcgagc cgacggtcac gaccccgggc tcgagcggcg
     7261 acagctcgcg gctgacgagc ccctgcagcg cgatcaccag ctgggccgcg acgtagatcg
     7321 ggtcccggcc ctggtgcggc gaggcaccgt gggcgccgat gccgcgcacg gtgacgtcga
     7381 cgctgtccga cgaagaagcg atcaaacccg gttcgagcga gatcttgccg gcggggtcgc
     7441 ccgccgagac gtgcaacgcc agcgcgtagt ccggcacgcc gaaacgcgag tacagaccgt
     7501 cggccagcat cgccttcgcg ccgccgattc gttcttcggc cggctgaccg acgaacatca
     7561 ccgtgccgga ccaatgctcc cgcagcgcgg cgaggcgtct cgccgtgccg accatcgccg
     7621 tcacgtgcac gtcgtggccg caggcgtgca tcaccggcac ctcggcgccg gtgatgtcga
     7681 cttgacgcgc ggtcgatgcg taaggcagcc ccgaactctc cgcgatcggc agaccgtcca
     7741 tgtcggcacg caccatcacc aacggaccct cgccgttcga cagcatgccg acgaccccgg
     7801 tgccgccgac cttctcgctc acctcgatcc ccgcgctgcg cagctcctcg gccagccgcg
     7861 ccgcggtgcg gaactcgcga aaggacagct cgggattgcg gtgaagctcg acgaacagat
     7921 cggcgaggtg ctcccgatag tcgcgctcga tcgccgcggc catgtcagcc ggcgtctcgc
     7981 ccgcaatcag ctgtggcgcg agcacgacca acatggccga gacccacttg ctcatcgccg
     8041 aacctccttc gttgcaggcg gcgatgatag acggcggttc tcccggcgcg cacaaaaaaa
     8101 cgggaaggcc atccggcgcg gcggcggccg atcgccgcat cgcgtgcaac cgggcgcgat
     8161 cgcgtgcgta ttacgacctc gacacgagac gccggaccgc cggcaggaag gcggccgact
     8221 tgattgcgag atcgcggggg tacggcgagt tttgatcgtg cggggcggcg agctgacctc
     8281 gctcgacctc gaccgatcgg gctgctcgat gctgcaggtc gccgacggct ggccttcggt
     8341 tggacgatcg ctccggtccc gccacgacgc gcgcgtgacg tcgagcgctg cccggttcgt
     8401 tcatcgcagg tgctcgtcga agaagcggat cacgtgcccc gtcgacagca cgcggttggg
     8461 gcgcttctgc acgccgtgcc cttcatcggg gaagatgatc agctcggccg ggacgtcgcg
     8521 tcgagcgacc gcctggtgga actgcagcgc ctctccgacc gggacgcgcg ggtcggtcgc
     8581 tccgtgcagg atcaacagcg ggtcctcgac gcgatcgacg aagtggatcg gtgacagctt
     8641 ctcgagcgcc tcgcggtccc gtcgcggatc gccgtactcg gtggtgcgga ggatg
//
LOCUS       DASPGN010000369         4373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_66554, whole genome shotgun sequence.
ACCESSION   DASPGN010000369 DASPGN010000000
VERSION     DASPGN010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66554"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2370)
                     /locus_tag="VD788_10600"
     CDS             complement(<1..2370)
                     /locus_tag="VD788_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV76757.1"
                     /translation="MSDESRFGTSFITSRPVAILMVFLAAVVFGALSFDRLPVTLMPE
                     LSYPTITVRTEYPGSAPEEVENDISRPIEESVGVIGGLKKLSSISRAGVSDVVLEFAW
                     DSEMSDATQDVLEKLDLVFLPEEAKRPLILHYDPALDPVMELSLSGEGRRFEGEAGLR
                     RLRRLADLRVKKELEPIKGVAAVRVRGGLEEEIHVRLEEEALRRSGISIQQVIDRLAQ
                     ENINVAGGTLQEGRTEYLVRTLNEYEDLEQIAATVVTRLDGREVRLRDLGQVLWANKE
                     REIVTRTDGGESVQIEIFKEGDANIVALAQRVKAAIGELDLEAEAPATAAAARGGEGG
                     QGSFAGLAAAIFAQEGARLQLVADRSLFIDSSIDEVINTAIIGGLLAVFILYLFLRNF
                     QSTAIVAISIPISLLITFAPLNLLGVSLNIMSLGGLALGVGMLVDSSIVVLESIYRCR
                     EEGDEVETAANRGTAEVRGAVFASTMTSVAVFFPMVFVEGIAGQAFGDLGLAVVVSLL
                     ASAAVALFFVPMLSGRPGLGLSVAVGFAATLLAVWLALNHRPAFLVTIAAIGLLLIVG
                     FLYSLYLAISGKLAVDPTTRKLLGIWNWASWRAFRRGLGWPVHATRSLVRRRRRATGG
                     RLRALAVLPRLAAGLLMIPVSLLYWSYLLVVRLVAGSLFEWLGKTVVIAFLGLVASSM
                     RFHLPLLGRLLAALSWLPLRLTGGVLERLHQSYPGVIRLALAHPLAIVVLVIACLWGS
                     WQVARSLESELLPEVHQGEFTVEVRFPVGTPLDRTERVVAPIEQAILAEK"
     gene            complement(2367..3566)
                     /locus_tag="VD788_10605"
     CDS             complement(2367..3566)
                     /locus_tag="VD788_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV76758.1"
                     /translation="MKTDVRIPVALLAVMIATAVAVGCGGSGKADASDGEVQAETATH
                     DDESAGEQDGEGDDKKNKKEEAIPVEVAELELGSIETVLSFSSNLEAEEQVKVYSEAK
                     RLITELLVEEGDRVAKGRVLLRLQDGEQLSALAKAGSQLDQARREYEQQHRLFTQELI
                     SEQAYNDAKYELDQLQIAYDDAKRELGYTEVQAPIAGTVTARLVNLGDQVQIGQHLFD
                     IVDFDSIVARIYVPEKYLTELRPGLAARLAAQTSETQAYSGTVDRISPIVDPKSGTIK
                     VTVEVGNQPGLRPGMYVDVDLVVAQRDDAVLVPKRAVVYDNDQMFVYRVDGEQRARRI
                     FIEAALSDKYFVQPREGLSSGDRVVVAGQAGLKDGALVKLPEGTQPDQTLEAAEDPSE
                     VIERASR"
     gene            3898..>4373
                     /gene="ftcD"
                     /locus_tag="VD788_10610"
     CDS             3898..>4373
                     /gene="ftcD"
                     /locus_tag="VD788_10610"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:NF019454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HXV76759.1"
                     /translation="MLIECVPNFSEGRDRAVIDAITAEIAHTDGAALLDVDPGRATNR
                     TVVTLAGPPAAVLEAAFRAIRKAAELIDMSKHRGEHARMGATDVCPFVPMQGATMQDC
                     IDLANRLAERVGDELGIPVYLYGEAARKPERKRLPDIRQGEYEALPKKLEDPAFAP"
BASE COUNT          737 a         1536 c         1396 g          704 t
ORIGIN      
        1 tttttcggcc aggatcgcct gctcgatcgg cgccaccacc cgctcggtgc gatcgagcgg
       61 cgttccgacg ggaaatctga cctcgacggt gaactcgccc tggtgcacct cgggcagcaa
      121 ctcgctttcg agcgaccgcg cgacctgcca cgagccccac aggcaggcga tgacgagcac
      181 gacgatggcg agcgggtgag ccaaggcgag gcgaatcacg cccgggtagg actgatggag
      241 ccgttcgagc actccaccgg tgagccgcag cggtaaccac gacagcgcgg cgagcagccg
      301 gcccagcagt gggaggtgga accgcatcga actggcgacg agcccgagaa acgcgatgac
      361 gaccgtcttg ccgagccact cgaacaggga cccggcgacg agccggacga ccagcagata
      421 gctccagtag agcagcgaga cggggatcat caacagcccg gcggccagcc gcggcaagac
      481 agccagggcc cgaagccggc cgcccgtcgc gcgacgacgc cggcgaacga gcgacctcgt
      541 cgcgtgcacc ggccagccca gtccgcggcg gaaggcgcgc cacgaggccc agttccagat
      601 cccgagcagc ttccgcgtcg tcggatcgac ggcgagcttg ccggagatgg cgaggtacag
      661 gctgtagagg aagccgacga tgagcaggag accgatcgcc gcgatcgtca ccaggaacgc
      721 gggcctgtgg ttgagcgcga gccacaccgc gagcagcgtg gcggcaaaac cgaccgcgac
      781 ggacaggccg agacccggtc ggccggacag catcggaacg aagaacagcg cgacggccgc
      841 ggacgccagc agcgagacga cgaccgccag accgaggtca ccgaatgcct ggcccgcgat
      901 cccttcgacg aagaccatcg ggaagaatac ggcgaccgac gtcatcgtcg acgcgaacac
      961 cgctccacgg acctcggcgg tcccgcggtt ggccgccgtc tccacctcgt cgccctcttc
     1021 ccggcaacgg tagatcgact cgagcacgac gatcgacgag tcgacgagca tgcctacgcc
     1081 gagcgccaaa ccgccgagcg acatgatgtt gagcgagact ccgagcaggt tcagcggggc
     1141 gaaggtgatc agcagcgaga tcgggatgct gatcgccacg atcgcggtgc tctggaagtt
     1201 gcgcaggaac aggtagagga tgaacaccgc cagcaagccg ccgatgatcg ccgtgttgat
     1261 cacttcgtcg atgctgctgt cgatgaacag cgagcggtcg gcgacgagct gcaatcgcgc
     1321 tccctcctgc gcgaagatcg ctgcggcgag accggcgaat gatccctggc cgccttcgcc
     1381 gccgcgggcg gccgccgccg ttgccggcgc ttcggcctcg aggtcgagct cgccgattgc
     1441 ggccttgacg cgttgggcca gcgcgacgat gttggcgtcg ccttccttga agatctcgat
     1501 ctgcacgctc tcgccgccgt cggtccgggt gacgatctcg cgctctttgt tggcccacag
     1561 cacctgcccg aggtcgcgca gccggacctc gcgcccgtcc agacgcgtga cgacggtcgc
     1621 ggcgatctgt tccagatcct cgtactcgtt cagcgtgcgc accaggtatt cggtgcgtcc
     1681 ctcctgcaac gtgccgcccg cgacgttgat gttctcctgg gccagccggt cgatcacctg
     1741 ctggatcgag atcccgctgc ggcgcaacgc ctcttcctcg agccgcacgt ggatctcttc
     1801 ctcgagaccc ccgcgcaccc gcaccgcagc cacgcccttg atcggctcga gctctttctt
     1861 gacacgcagg tcggccagcc ggcgcagccg gcgcagaccg gcctcgccct cgaaccgccg
     1921 gccctcgccg gacagactga gctccatcac cgggtcgagc gcggggtcgt agtgcaggat
     1981 caacggacgc ttggcttcct cgggcaggaa gaccaggtcg agcttctcga gcacgtcctg
     2041 cgtcgcgtcg gacatctccg agtcccaagc gaactccagc acgacgtcgc tgacgccggc
     2101 gcggctgata ctgctcagct tcttcaggcc gccgatcacg ccgaccgact cttcgatcgg
     2161 ccggctgatg tcgttctcga cctcttccgg cgccgacccc gggtactcgg tacgcaccgt
     2221 gatcgtcggg tagctcagct cgggcatcag cgtgaccggc aaccggtcga acgacagcgc
     2281 gccgaacacc accgcggcga gaaagaccat caggatcgcg acgggtcgcg acgtgatgaa
     2341 gctcgtgccg aaccgtgatt cgtcgctcaa cggctcgccc tttcgatcac ctcgctcgga
     2401 tcttcggccg cctcgagcgt ctggtccggc tgcgtgccct ccggcaactt gaccagcgcg
     2461 ccgtccttca gcccggcctg ccccgcgacg acgacgcgat cgccgctgct cagaccctcg
     2521 cgcggctgca cgaagtactt gtcgctcagt gcggcttcga tgaagattcg ccgcgcgcgc
     2581 tgctcgccgt cgactcgata gacgaacatc tggtcgttgt cgtagaccac cgcgcgcttg
     2641 ggtaccagca cggcgtcatc acgctgggcg acgacgagat cgacgtcgac gtacatcccg
     2701 ggacgcagac ccggctggtt gccgacctcg accgtgacct tgatcgtccc cgacttggga
     2761 tcgacgatcg gcgaaatgcg gtcgacggtt cccgagtagg cctgcgtctc gctggtttgc
     2821 gcggccagcc gggccgcgag cccgggacgc agctcggtca gatacttttc cgggacgtag
     2881 atccgcgcca cgatcgaatc gaaatcgacg atgtcgaaca ggtgctggcc gatctgcacc
     2941 tgatcaccga ggttgaccag gcgtgcggtg acggtgccgg cgatcggcgc ctggacctcg
     3001 gtgtagccca actcacgttt cgcgtcgtcg taggcgatct gtagctggtc cagctcgtac
     3061 ttggcgtcgt tgtaagcctg ctcgctgatc aactcctgcg tgaacagccg gtgctgctgc
     3121 tcgtactcgc gtcgcgcctg atcgagttgg ctgccggcct tggccagcgc gctgagttgc
     3181 tcaccgtcct gcagtcgcag caacacccga cccttggcca cgcggtcacc ctcctcgacg
     3241 agcagctcgg tgatcagccg cttcgcctcg gaataaacct tgacctgttc ctcggcctcc
     3301 agattcgacg aaaagctgag caccgtttcg atcgagccga gctcgagctc cgcgacctcg
     3361 accggtatcg cctcttcttt tttgttcttc ttgtcgtcgc cctcgccgtc ctgctcaccg
     3421 gccgattcgt cgtcgtgcgt ggccgtttcg gcttgcacct cgccgtccga cgcgtccgcc
     3481 ttcccgctgc cgccgcagcc gacggcgacg gccgtcgcga tcatcaccgc caacaacgca
     3541 accgggatcc gcacgtcggt tttcacggct cgctcctgcc ttttgtcttg tttcgcccct
     3601 gtcggaggcg gtggtgtgga ccgggaacct cgtcgccgac tcaataaacg gctcgcgact
     3661 tccgctggtt ccatcggccg tacccgccgc cccggtggcc gctacaccca actagacggg
     3721 cgacaggccg gggggattac atccgggacc gatatcctcg cgctcgccgt tcccgtgccc
     3781 gggttcgacg cccgccatcg cttcaactta tcaaacggga acgatttacg ccgccgaaca
     3841 cgagctgggc tagaatcgtc cgaacccgcg tctggccggc cgccgaggag aatccggatg
     3901 ctgatcgagt gtgtgcccaa tttttccgag ggccgcgacc gcgccgtgat cgatgcgatc
     3961 acggccgaga tcgcgcacac cgacggcgcc gccctgctcg acgtcgatcc cggccgggcg
     4021 accaatcgca cggtcgtcac gctggccggc cccccggcgg cggtgctcga ggccgcgttt
     4081 cgcgccatcc gcaaggcggc cgagttgatc gacatgtcga agcatcgcgg ggagcacgcc
     4141 aggatgggtg cgaccgacgt ctgcccgttc gtgccgatgc agggcgcgac gatgcaggac
     4201 tgcatcgatc tggccaatcg gctggccgag cgcgtcggcg acgagctcgg gattccggtc
     4261 tacctgtacg gagaagcggc acgcaagccg gagcgcaagc ggctccccga catccgccaa
     4321 ggggaatacg aggccctgcc gaagaagctc gaggacccgg cgttcgcgcc cga
//
LOCUS       DASPGN010000370         4200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_418288, whole genome shotgun sequence.
ACCESSION   DASPGN010000370 DASPGN010000000
VERSION     DASPGN010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418288"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VD788_10615"
     CDS             complement(<1..495)
                     /locus_tag="VD788_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXV76760.1"
                     /translation="MCPVEPWRIGVDLGGTKIEAALLDGAGRIVRRRRRATPRGDYRA
                     TLAALVDLVGSLRAGLDRPTTVGVCGPGAVSPATGRIKNANSVWLNGMPLREDLEREL
                     GHGVRLANDADCFALSEAVDGAARGAGTVFGVIVGTGVGGGVVVGGRLIRGPNAIAGE
                     WGHNP"
     gene            complement(482..>4200)
                     /locus_tag="VD788_10620"
     CDS             complement(482..>4200)
                     /locus_tag="VD788_10620"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HXV76761.1"
                     /translation="TDGIGVAPDGRLRRVKLLSTGALRGRAAADGAGDRLRVDASWDC
                     DAAPLRGLILRRPGRPRQLGRVASYSADDRTLELQAPLTQPLAAGETFELVSDEDAPL
                     FAARLLMGSAAGEPLPGARIRLATTLGTNALLERHGARTALFITAGFGDLLRIGTQQR
                     PDLFALDIVRPEPFYETVVEVPERIEASGRVLRALSTAEPAEHARRLVRCGLRTAAVA
                     LMHAYRNPAHERELARLLTECGFTHVSCSHELAPLIKLVPRAQTAVVDAYLAPVIHGY
                     LTRVGAAAGDGLLVMTSAGGLSAPDRFRPKDSLFSGPAGGVVGAAHAGRRAGYDRLIS
                     FDMGGTSTDVARIDDDYEYRYEQRIGDAELIAPGLAIETVAAGGGSVCWLDGARLRVG
                     PESAGAAPGPACYGAGGPLTLTDVNLLLGRLDASRFGIPVDRRAAERRLGQLLAALAD
                     HTGEPVDAEAVLRGLIAIADETMADAIRRISLRRGYDPSDYVLVAFGGAGGQHACGVA
                     DRLGMTRVVVPPDAALLSAAGLGHAVLERFAERQVLEPLERCRAPLARWIDELAREAS
                     RDLLAAGAEPDELAVRRRLLQLRLAGQEATIGIDWQPAGDPAADFERAYVGLYGHAPE
                     SRSIELESIRVIVSAAAPPGSASTGDVADGGDAADVARRARYDRGELAVGRSVPGPAL
                     IVEPHSTTVVDERWTATVLSDGALGLARATRVEEQEDGEHERPESIRLELFTQRFTNL
                     VREMGERLERTAVSTNVKERLDFSCALLDPHGSLVVNAPHIPVHLGALGLCVRRLGEA
                     IRLRPGDVAVTNHPAFGGSHLPDVTVVTPVFVNESGGRMLGYVASRAHHAEIGGIRPG
                     SMPPAATSLAEEGVVIPPTLLIEQGRPHWERMRRLLLDAEFPTRAVEDNLADLRAAVA
                     ANHAGGCALRELARVHGADTVLRFMEQLDRIADDRIRCALRKLGDGVYGARELLDDGS
                     PLVVRAECEDGRITLDFTGSSGVHPGNLNATPAIVRSAVLYVLRLLIDRPLPLNEGLL
                     RAATIRVPSGILDPPFSDDPRSSPAVVGGNVETSQRLVDTLIRALGLAACSQGTMNNV
                     IFGNASFGYYETIGGGCGAGPGFDGASAVHSHMTNTRITDPEVVEHRYPVRLERFAVR
                     PDSGGAGFHRGGDGLVREFLFLDEVELSLLTQHRNQAPYGMQGGQAGRTGAQRVIRAN
                     GETLELGAVDGCSIRRGDRLIVETPGGGGWGVPG"
BASE COUNT          626 a         1565 c         1451 g          558 t
ORIGIN      
        1 cgggttgtgg ccccattcgc cggcgatggc gttcggcccg cggatcagcc ggccgccgac
       61 caccacgccg ccaccgaccc cggtgccgac gatcacaccg aacaccgtgc ccgcgccgcg
      121 cgccgcaccg tcgaccgctt cggacagcgc aaagcagtcg gcatcattgg ccagccgcac
      181 gccgtgaccg agctcgcgct cgaggtcttc gcgcaacggc atgccattga gccacaccga
      241 attggcattc ttgatccgac cggtcgcggg tgagaccgcc ccgggcccgc agacgccgac
      301 ggtcgtcggc cggtcgagcc cggcgcgcaa cgacccgacg agatccacca gcgcggcgag
      361 cgtcgcgcgg taatcaccgc gcggcgtcgc gcgccgacga cgacggacga tccggcccgc
      421 accgtcgagc aacgcggctt cgatcttggt cccgccgaga tcgacgccga tccgccacgg
      481 ctcaaccggg cacaccccag cctccgccac ccggcgtttc gacgatcaag cggtcgccgc
      541 gccgaatcga gcagccgtcg accgccccca gctcgagcgt ctcaccgttc gcgcggatca
      601 cgcgctgcgc gccggtgcgg ccggcctgcc cgccctgcat gccgtacggg gcctggttgc
      661 gatgctgcgt cagcagtgac aactcgacct cgtcgaggaa caggaactcg cgcaccagcc
      721 cgtctccccc acggtggaag ccggccccgc ccgagtcggg ccggaccgcg aatcgctcga
      781 gccgtaccgg gtaacgatgc tcgacgacct cggggtcggt gattcgtgtg ttggtcatgt
      841 ggctgtgcac cgcgctcgcg ccgtcgaagc ccgggccggc gccgcagcct ccgccgatcg
      901 tctcgtagta gccgaacgag gcgttgccga agatcacgtt gttcatcgtg ccctggctgc
      961 acgcggccag gcccagcgcg cgaatcaacg tatcgaccaa gcgctggctg gtctcgacgt
     1021 tgccgccgac caccgccggc gaagaccgcg ggtcgtcgct gaacggcggg tcgaggattc
     1081 cgctcggtac gcggatcgtc gccgcgcgca gcagcccctc gttcagcggg agcggccggt
     1141 cgatcaacag ccgcaagacg tacagcaccg cgctgcgcac gatcgccggg gtggcgttga
     1201 ggtttcccgg gtgcaccccg gatgacccgg tgaaatcgag cgtgatccgc ccgtcctcgc
     1261 attcggcgcg gaccacgagc ggcgagccgt cgtcgagcag ctcgcgtgcg ccgtagacgc
     1321 cgtcgccaag cttgcgcaac gcgcaacgga tccgatcgtc ggcgatgcgg tcgagctgct
     1381 ccatgaatcg caggacggtg tccgcaccgt ggacgcgcgc gagctcgcgc agggcgcacc
     1441 cgccggcgtg attcgccgcg acggccgcgc gcaggtccgc cagattgtcc tcgaccgccc
     1501 gcgtcgggaa ctccgcgtcg agcaacagcc ggcgcatccg ctcccagtgc gggcgaccct
     1561 gctcgatcag cagcgtcggc ggaatcacca cgccctcctc ggccaggctc gtcgccgcgg
     1621 gcggcatcga accggggcgg atcccgccga tctcggcgtg atgcgcgcgc gaggcgacgt
     1681 agcccagcat ccgtccaccc gactcgttca cgaagaccgg ggtgacgacg gtgacgtcgg
     1741 gcagatgcga gccgccgaac gccgggtggt tggtcaccgc gacgtcgccc gggcgaagcc
     1801 ggatcgcctc gccgagccgt cgcacgcaca agccgagcgc gccgaggtgc accgggatgt
     1861 gcggcgcgtt cactacgagc gagccgtgcg ggtcgagcaa cgcgcaggaa aagtcgagcc
     1921 gctctttgac gttggtcgac acggcggttc gctcgaggcg ttcgcccatc tcccgcacca
     1981 ggttggtgaa gcgttgcgtg aacagctcga gacggatcga ctccggtcgc tcgtgctcgc
     2041 cgtcctcctg ctcctccact cgcgtcgctc gcgccagtcc gagcgccccg tccgacagca
     2101 ccgtcgccgt ccaccgctcg tcgacgaccg tcgtgctgtg gggctcgacg atcaacgccg
     2161 ggcctggcac gctccgaccg acggccaatt caccgcgatc gtatcgtgcc cgtcgggcca
     2221 cgtcggccgc gtctccgccg tccgcgacgt cacccgtcga agccgagccg ggtggcgcag
     2281 cggcggagac gatgacgcgg atcgactcga gctcgatcga gcgcgattcg ggggcatggc
     2341 cgtagagccc gacgtaggca cgctcgaaat ccgccgcggg gtcgccggcg ggctgccagt
     2401 cgatcccgat cgtcgcctcc tgtccggcca gccggagctg gagcaggcgg cggcggaccg
     2461 ccagctcgtc gggctcggcg ccggcggcga gcaggtcgcg gctcgcctcc cgtgccagct
     2521 cgtcgatcca gcgcgcgagc ggcgcgcggc aacgctcgag tggttcgagc acttgacgtt
     2581 cggcgaagcg ttcgaggacg gcgtggccca gcccggccgc cgacagcagg gcggcgtcgg
     2641 gtggaacgac gacgcgtgtc atgccgagcc ggtcggccac gccacatgcg tgttggcccc
     2701 cggcgccgcc gaacgccacc agcacgtagt ccgacgggtc atacccgcgt cgcagcgaga
     2761 tccggcggat cgcgtcggcc atcgtctcgt cggcgatcgc gatcaaaccg cgcagcaccg
     2821 cttcggcgtc gaccggctcg ccggtatggt cggcgagcgc ggccaacagc tgtccgagcc
     2881 ggcgctcggc ggctcgccgg tcgacgggaa tgccgaagcg cgaggcgtcc aaccggccga
     2941 gcagcagatt cacgtcggtc agcgtcagcg gcccgcccgc tccgtagcag gccggcccgg
     3001 gtgccgcgcc cgcgctctcc ggaccgaccc gcaaccgcgc accgtcgagc cagcagaccg
     3061 agccaccgcc ggctgcgacg gtctcgatcg ccagacccgg tgcgatcagc tcggcgtcgc
     3121 cgatccgctg ctcgtagcga tactcgtagt cgtcgtcgat acgggccaca tcggtgctcg
     3181 tgccacccat gtcgaagctg atcagccggt cgtagccggc ccgacgcccg gcatgggcgg
     3241 cgccgacgac accccccgcc gggccgctga acaggctgtc cttcggccga aaacgatcgg
     3301 gtgcgctcag tccgccggca ctggtcatca ccagcagtcc gtcgccggcg gcggcgccga
     3361 cccgcgtcag atagccgtgg atgaccggcg ccaggtaggc gtcgaccacc gcggtctgcg
     3421 cgcgcggcac cagcttgatc agcggcgcca actcgtgcga acaggagacg tgggtgaatc
     3481 cgcactccgt cagcagccgt gccaactcgc gttcgtgagc cggattgcga taagcgtgca
     3541 tcagcgcgac ggcagccgtc cgcaggccgc agcggaccag acgccgagcg tgctcggccg
     3601 gctcggccgt cgacagcgcg cgcagtacgc gaccgctcgc ctcgatccgt tcgggcacct
     3661 cgaccaccgt ctcgtaaaac ggctctggcc tgacgatgtc gagcgcgaac agatcggggc
     3721 gctgttgcgt gccgatccgc agcaggtcgc cgaagccggc cgtgatgaac agcgcggttc
     3781 gcgccccgtg gcgctcgagc aacgcgttgg tgccgagggt ggtcgccaga cgaatgcggg
     3841 cgccgggtag cggctcgccg gccgcgctgc ccatcaacag gcgcgcggcg aacaacggcg
     3901 cgtcctcgtc cgacacgagc tcgaacgtct caccggcggc gagcggctgg gtcagcggcg
     3961 cttggagttc gagcgtgcga tcgtctgccg agtacgaggc gacacgaccc aactggcggg
     4021 gacgtcccgg acgtctcagg atcagaccgc ggagcggcgc ggcgtcgcag tcccacgacg
     4081 cgtccacgcg caatcggtcg cccgctccgt cggcagcggc ccgtccgcgc aacgcgccgg
     4141 tgctgagcag cttgacgcgg cgcaaccgcc cgtcgggcgc cacgccgatg ccgtcggtga
//
LOCUS       DASPGN010000371        18103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_418306, whole genome shotgun sequence.
ACCESSION   DASPGN010000371 DASPGN010000000
VERSION     DASPGN010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 18103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18103
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418306"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VD788_10625"
     CDS             <1..801
                     /locus_tag="VD788_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76762.1"
                     /translation="TLGATLDRIAGEKAGIVKPTMPVVSGVTQQAAVDVIRRTCRERG
                     ARFIDARHAVRFEREDASGLVFASQRTRYRGIRLALDGRHQIDNARVAVAAYECLMER
                     IGRRPRPAAVRQALSRVRWAGRLERLVPAAGPPALVFDAAHNPAGVEALARHLRLAAS
                     TPPVLLFGATSGKPLERMLEPLAALVAAAVISRPQVERGLDAEEVAVVARRFLSVVEV
                     VPDPALAVVRARELAGCDRDVLVAGSLYLVGQVMGLLAERTVPGPVAM"
     gene            812..1951
                     /locus_tag="VD788_10630"
     CDS             812..1951
                     /locus_tag="VD788_10630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV76763.1"
                     /translation="MDPVNWAGWLVGLLLFALGLQRLRDLAAVPPLPDRTDPRSPRVS
                     MIVTARDELARIETSVRRAIAQREVELELIVVDDRSADGTREVLERLAREYARLRVLR
                     VDELPDGWLGKPHACHVGAEQARGEWLLFSDADAWMTDRVVARAVDVARREGADHLCL
                     FPGESHASIAARATLLNFSAVLLHYAARANRDERRSMIGVGAFNLVRAEAYREIGGHR
                     RLRFEVVDDLKLGLLLRRAGFRSRAFGAADDVEVHWAPTVPRVVHALEKNMFAMLEFS
                     TPRGLLAIAGLATLVAAALVTPFLGGSGALVASLGWAVSLVPALVMARHTRWGVLPVL
                     LTPFVSPVMVWVVANSVYRTLRRGGICWRGTFYAIDALRDGQVRP"
     gene            2026..2652
                     /locus_tag="VD788_10635"
     CDS             2026..2652
                     /locus_tag="VD788_10635"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76764.1"
                     /translation="MGRAIKVEAPQLRLDPAAIAESIDWPTVFGRGGPVEIEIGIGKG
                     RFLLEIARLRPEVLHLGVEWANKYLRVAERRAARRALDNVRFARVDGRELIRRAVPDA
                     SVSAYYVFYPDPWPKKRHHKRRFFRPATLDDLARTLVAGGALHAATDHAEYWSVIETL
                     MDGDDRFERSAEFGGPGFPLPVDGPLTNYEAKYGVEGRSRNRGSWRRR"
     gene            complement(2660..3409)
                     /locus_tag="VD788_10640"
     CDS             complement(2660..3409)
                     /locus_tag="VD788_10640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV76765.1"
                     /translation="MDLAEEYRQQFQWRDWATALAALPSVEERVVIDLGCGVGDLAAE
                     LAARGARVVGLDGNRELLAVARSRRIPNAEFRQADLGALPDLGIRADGLWCSFVAAYF
                     PSLAGTLSRWAQNLEPGGWVMLVEVDDLFGHEPLGTAARARFDAYVEEALAAGRYDFR
                     AGSKLAQHLGAAGFRIESALRLRDEEFSFDGPAAAGVVEAWSRRLERMVLLEQRWGES
                     YRSVRDEFLGCLRRTDHRSTATVRACLARKS"
     gene            3520..3596
                     /locus_tag="VD788_10645"
     tRNA            3520..3596
                     /locus_tag="VD788_10645"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3554..3556,aa:Trp,seq:cca)
     gene            3669..4718
                     /locus_tag="VD788_10650"
     CDS             3669..4718
                     /locus_tag="VD788_10650"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH and helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXV76766.1"
                     /translation="MGVKTFVRGELIEIIEWTDATRDTLACRFPDEDRAIKRGAQLIV
                     RESQVAQFVYLGQFGDTFGPGKHRLVTDNIPILTRLASWRYGLESPFKADVYFVNTRL
                     FTGNKWGTSNPLMMRDDDLGVVRARAFGTYDCRVVDAKRFLREVAGSDHDFRLEEFAE
                     TMRSRLVSVFSDALATSRVPLLDLAARYTELGHALLPLINPVMAGKYGVEIDNFVVEN
                     VSVPSEVEQAIDKRSSMAAVGDIDEYVKFQLGRGLELGGGGAAGMASEMAVSLAIAQE
                     MMQRQRSAPAATEAGASDPRSIPDLLSVEQVAELLGVDRADVTSVIESGELEAKRIGR
                     SWRVKRSALERYLAD"
     gene            complement(4719..5642)
                     /locus_tag="VD788_10655"
     CDS             complement(4719..5642)
                     /locus_tag="VD788_10655"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HXV76767.1"
                     /translation="MAVESPSALKFLVACDGCRRQYDAGALAPGSRFHCSCGATVVVP
                     RRKVFEAEVVRCSSCSAPRTGNRAACTHCGADFTLHERDLQTICPQCMARISNLARFC
                     HHCATPIVPQGRAGEPTGVDCPVCGSGRTLNSRSLAQDRVTVLECGACAGMWVGRRAF
                     ELIAEKARGVAASADEPLAATAAGGGASPAAQHGSLYRRCPECNQMMNRRNFGRRSGI
                     VIDSCKDHGIWFDAEELDRILRWIRQGGEARSRKRADEERGRGVSAADRFLNQRLDRM
                     AGQGSTSIRVERTGFDRDDLVGGMIRLLFDL"
     gene            5714..6268
                     /locus_tag="VD788_10660"
     CDS             5714..6268
                     /locus_tag="VD788_10660"
                     /inference="COORDINATES: protein motif:HMM:NF015239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HXV76768.1"
                     /translation="MALKLFLTGDPGCGKTTAVRRVVERLRGRVRMTGFITEELRRDG
                     RRIGFRGVTLDGTPFPLADRDARGELRVGPYRVVLDGLESIGLAALEPGGAQLVVLDE
                     VGKMELLSSRFRERVTALVEGTTPLLATIALHGVGFIRRLRHDPRVTLVKMSRHDSDT
                     AVGDAIRRLRDAGVVPRRNGTGRS"
     gene            complement(6292..7320)
                     /locus_tag="VD788_10665"
     CDS             complement(6292..7320)
                     /locus_tag="VD788_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76769.1"
                     /translation="MSASFMRLASRLGRPAAVVCTVLLGVACGGGDPRGAASVDLAAA
                     DYVGIEACSGCHLGVYTSYAGTGMARSVRMAEIDPRPAGPARLVVGSGNHARAIFHQD
                     AGDATRPASCSLAPGHEWHECPDDPRTPSHLAGAPLEHCLGCHAAVAREGVAADGVDC
                     ERCHGPGSIHVDRWRSATGSDGEADPTIVNPRRLPHAERMAVCLRCHFGAPPGLPHED
                     RAAAAARVTEFRPGPFLEGFATDAQGARLMSSRCYVESGGGVDCLTCHDPHVTVYHDD
                     RPPERFRRACLRCHAADDCTLEPGTRAATRPPDDCTACHMRRAEPEDQAGTHYTDHWI
                     RRPIDRSR"
     gene            complement(7317..8696)
                     /locus_tag="VD788_10670"
     CDS             complement(7317..8696)
                     /locus_tag="VD788_10670"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HXV76770.1"
                     /translation="MKESDELRRRSRWLLRVMEWNDSRARYPNIWIDGMPRHVLAFER
                     IASRLRLGDLIAIYYPASQRHTERSEKFLGLSRAVGVRLAETKGYAWVDLSTVHRFAS
                     PLAFDQPPRRVFLCCDPGWPDAEVRLFGKVWDAALAEGWVPEPEDRESGSRVEAAGEA
                     GRPAEPAPAVAAAVPRRGVGRRMPAGRLFAGVDYSGDMRDPREATWLAIVELCGDRLN
                     VVRLDATGRHGLEGYLREPDATLMNVEAIGLDFPFSVPLGFAERLLGGAFPDEGWWAL
                     ARKMETLSRPEYLVALQEYRDQHGESRRHTDEVADAFSPLHRVDPDRGPMTFHGIRMI
                     AEDRSRYAVRPFESAQGRLLLEVYPGGFTRRLSPLAVSEKANGRGRRQRILSALGHAG
                     YLPTTCDARTARKCLERGDALDAVIAARQAAVAVMTGEVERTPEQLAPDAAERVRREG
                     WIYGLAEPE"
     gene            8969..10903
                     /locus_tag="VD788_10675"
     CDS             8969..10903
                     /locus_tag="VD788_10675"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HXV76771.1"
                     /translation="MPGTNVDLPPTVILVGNPNVGKSVVFKHLTGRHVTISNYPGTTV
                     EITRAPAMVGGRRTELFDTPGINDLAQESEDAQVTREIIARHPDATILQVADAKNLRR
                     ALLLTIQLAELGRPMVLVLNMMDELERSGARIDTPALARTLGVPVVSTVAIARHGIDQ
                     LVASIPDAKPAGVAVVVDGPPREVFVRNQMRMDRANEILAESYRLERPARPPFSVRLG
                     FWAMDPLKGLLVLLAALLFTFWFVGLIGAGTFVDLLETGLFHQGLNPLAIRAADALLP
                     FDHTHSPEPVTLEVSIPLSPAHGIPLASLEREVATTAYVLDGEPADGPLAASVRLVHD
                     FLVGEYGMITMGLSYALAIVLPIVITFFLVFSVLEDSGYLPRLSIMVNRLFRLMGLSG
                     KAVLPMVLGLGCDTMATMTTRILETRRDRLITSLLLALAIPCSAQLGVLLAMMAQLSV
                     GAAVLWVVLIVGVMLLVGWLSSKVFPGERSDFILELPPIRRPQIGNVVAKTFARIEWY
                     LKEVIPLFVAGTAILFVLDRTHVLTWIAAAGRPLVSGWLGLPADAINAFLIGFLRRDF
                     GAVYLLDAATGSDAILSPLQIFVSMVTITLFMPCIANLFMIAREHGKRIALYMTLFIF
                     PFAFLVGGLVHLVGRALGYR"
     gene            10904..11347
                     /locus_tag="VD788_10680"
     CDS             10904..11347
                     /locus_tag="VD788_10680"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HXV76772.1"
                     /translation="MKHAPLYLHCSLCGLDFEKTDTVCQRGCPLGVACGHVRCPNCAF
                     EFPEPRRPAQWLRRILGRRGRLDLRALEGATVTLDRLDSGTRCRLQHLGCRSHGRRNT
                     LTVFGMTPGTEIELLQRQPSFVVRVGETEVGLDEEIAREIYVKPI"
     gene            11399..12547
                     /locus_tag="VD788_10685"
     CDS             11399..12547
                     /locus_tag="VD788_10685"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HXV76773.1"
                     /translation="MSTPRRIPAVHVAAFMILAACSASSAAPGPLVLVGGGLDESNEP
                     VYREILGLRAGPRPICVLPTASDEPQQAADRFAARFERYGGPGTATVVRLKPGGADKP
                     KIARRLEQCGGFFFLDGDPSRIVDTLRGGGRTSLAEQAILRVHRRGGVIAGSNAAAAA
                     MSDPMIAGETGSDDALRHGVSSSAGEAGLWVRDGMGFLDAGLTDPHHISAGRTGRLLV
                     ALAALDGVDRGFGIDDDTALVVDGTRARVVGSSQAIVVEKVSAGSGRLRLAGARFRVL
                     VLGNGDGYHLDDRTAFVDAGKNVLRLPDRVPTPPDGPWLASGLPLFLVELSVSSASEA
                     TFESRGFRFRLSKEAGFEARAWELPDGRHLPHGFTAGWFLLDVSEATP"
     gene            12658..14034
                     /locus_tag="VD788_10690"
     CDS             12658..14034
                     /locus_tag="VD788_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV76774.1"
                     /translation="MWRCCLPILVSLSIPLGSAAGDEPGEPIGPADAVRLALEHNPRL
                     LAADAGLRAAEADRRAASASGLPRIEFTEDWVRSNNPVFVFSSKLLQGVFATDDFAID
                     VLNEPEPITNSTARLSIYQSIWNGRNSPEKKTAVAGVAVAESSQRKARDEVVFTTLEA
                     YWALASARRMMEVTADGVAAAEANLALARELVDAGLAVPSDRLAAEVRLADVRATSVR
                     AEYGVEVAHAALLRALGEDRPEMRYVPIETDRLPELGDDSLERRTEQAMLGRADLAEL
                     DRRIEQARIGARSARAGRYPEIGAAGSYEWNGERLLAADGENWAVGLALRMTIFDGHR
                     TDARVQRAAAELAQLEAMRVALAQGVRLEVASAWAERESAARRLELAATALEQAGEVM
                     RIVRERYGEGVALVVELLSAEAALTAAQAQQVVAVGELWLADAGLDLASGTDPIPDLP
                     SVTTGGPG"
     gene            14051..15130
                     /locus_tag="VD788_10695"
     CDS             14051..15130
                     /locus_tag="VD788_10695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV76775.1"
                     /translation="MKTSSLRSVVMLLSLVLGGCAGDGGRPPAEPRPRVAVDVERVAV
                     ESWRDGLELTAGVLPAVRATPGTVLMGRVDRVLVEEGDVVERGQTLARIESRDVAARL
                     AQARAGVAAARAAEQNARRMHERMERLHANSAASDKNLEDAEAAHEAALAQLAAAEEA
                     VKVAEVQVDYSEIVAPFAGVVVDKGIEAGDTAAPGMPLFVIDDLSKVKIEVQVPESAA
                     ASVAVGDPVEVEFRGERFDGRLSELVPAADPRSRTVTMRALLDNPERRLRAGMFARLV
                     LPGGVRDALTVPSSAIVRKGPLTGVFVVGSDASDRPVAGLRWVTLGTERAGRVEVLTG
                     LDAGERVVSAPDGRLEDGQPVEVGE"
     gene            15127..>18103
                     /locus_tag="VD788_10700"
     CDS             15127..>18103
                     /locus_tag="VD788_10700"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV76776.1"
                     /translation="MTGIGISGRIAGAFIGNKLTPLLAIAALVLGLMATLATPREEEP
                     QIVVPMIDVMIGWPGASAEDVERLVTVPLERALREIPEVRYVYATSRPSAAMLIAQFY
                     VNSDPERALINVRDKAANAGGRLPAGVAPATITSRSIDDVPILALTLASGRYDPLTLR
                     RIAAEIEEDVRQIPGVSTTTLIGGLGREIRVELDLERLAARGLAPAVVLDALERSGVA
                     LPAGSVGSARGELLIETGELLRSAAEVGATVLASPDGSPVHLRDVAAIHDGAAEPRLY
                     VMHAEHGRPSQPAVTLSVAKIEGANATDVAQAVLQRVERMRGRIVPEDVTLTVTRDYG
                     ETAKQKSDELILHLIVATASVVLLMAVSLGVRAGLIVGVAVPVTLSLTLLIYYLWGYT
                     LNRVTLFALIFSIGILVDDAIVVVENILRHLRMKKLPPLQAAVFAVDEVGNPTILATF
                     TVIAAILPMAFVSGLMGPYMRPIPVGASFAMLISLLVAFTVSPWIALRVLRRDDVQAG
                     RASDAAAAEGWHARAYRRLMRPLIERPLYRWVIFAGVVVLLLGVVALVPLKVVTVKML
                     PFDNKSELQLIVDAREGTTLEDTLAAARAMADRLAVEPEVRDIQIYAGTSAPFNFNGL
                     VRHYFLRSGANVADLQINLMPKEQRRLSSHEMARKVRPLVVEIARRHGVRVKVAEIPP
                     GPPVLSTLVAEVYASDRPTQIAIAERVLAIFESTDGVVDSDWYVEADQPKLRFVVDRE
                     KAALAGVDPEQIAITLRLAVDGIDATMAHPGDEREPVPIRVTLPRAARARDEVLRDIR
                     LIAADGSVVPLSEVARVESTTARPYLYRKNGRAVTYVIGDVAGSEESPVYAILAMSDR
                     IRQIELSALGAIDERMVDDPLAGDREAVVWDGEWQITYEVFRDLGVAFAAVLVLIYVL
                     VVAWFQSFTVPFVIMAPIPLTLVGILPAHAATGAFFTATSMIGMIALAGIIVRNSILL
                     VDFITLGLERGQPLRDAVVA"
BASE COUNT         2565 a         6224 c         6454 g         2860 t
ORIGIN      
        1 acgctcgggg cgacgctcga ccggatcgcc ggcgagaaag cgggcatcgt caagccaaca
       61 atgcccgtcg tcagcggcgt cacgcagcaa gcggcggtcg acgtgatccg ccgaacctgt
      121 cgcgagcgcg gtgcgcggtt catcgacgcc cgccacgccg tgcgcttcga acgcgaggac
      181 gcgagcgggc tcgtcttcgc gagccagcgc acgcgctacc gcggcatccg gctggcgctc
      241 gacggacggc accagatcga caacgcccgc gtcgccgtcg ccgcctacga gtgcctgatg
      301 gagcgcatcg gccgccggcc gcgaccggcg gcggtgcgcc aggctctctc tcgcgtccgc
      361 tgggccggcc gtctcgagcg gctcgtgccg gccgccggcc caccggccct cgtgttcgac
      421 gccgcgcaca acccggccgg cgtcgaagcg ctggcgcgcc acctgcggct ggctgcatcc
      481 accccgccgg tactgctgtt cggggcgacg agcggcaagc cgctcgagcg gatgctcgag
      541 ccgctcgccg ctctggtcgc ggcggccgtg atcagccgcc cacaggtcga gcgcggcctc
      601 gacgccgagg aagtcgccgt ggtggcccgc cgattcctgt cggtcgtcga ggtcgttccc
      661 gacccggcgc tcgccgtcgt ccgcgcgcgc gagttggccg gctgcgatcg cgacgtgctg
      721 gtcgcgggct cgctctatct cgtcggtcag gtgatgggat tgctcgcgga gcgcaccgtg
      781 cccggcccgg tggcgatgta gggcggctgg ggtggacccc gtcaactggg ccggctggct
      841 cgtcgggctg ctgttgttcg ccctcggcct acagcggttg cgcgatctgg cggcggtgcc
      901 gccgctaccc gatcggaccg acccccggtc tccgcgcgtg tcgatgatcg tcaccgcgcg
      961 cgacgagctg gcgcgcatcg agacctcggt gcgccgcgcg atcgcgcaac gagaagtcga
     1021 gctcgagctg atcgtggtcg acgaccgctc ggccgacggc acgcgggagg tgctcgagcg
     1081 cctggcgcgg gagtatgcgc gactgcgcgt cctgcgggtc gacgagctac ccgacggttg
     1141 gctgggcaag ccgcacgcct gccacgtcgg cgccgagcaa gcacgcggcg agtggctgct
     1201 gttcagcgac gccgatgcgt ggatgaccga ccgggtcgtc gcgcgagcgg tcgacgtcgc
     1261 gcgccgcgag ggagccgacc acctgtgtct cttccccggc gagtcgcacg ccagcatcgc
     1321 cgcgcgcgcc acgctgctca acttctccgc cgtgctgttg cattacgccg cgcgggccaa
     1381 ccgcgacgag cggcgatcga tgatcggcgt cggcgcgttc aacctggtgc gggccgaggc
     1441 ctatcgcgag atcggcggcc accgcaggct ccgcttcgag gtcgtcgacg acctcaagct
     1501 cggcctgctg ttgcggcgcg ccggcttccg ctcgcgcgcg ttcggcgcgg cggacgacgt
     1561 cgaggtgcac tgggcgccga ccgtaccccg cgtcgtccac gcgctcgaga agaacatgtt
     1621 cgcgatgctc gagttcagca cgccgcgcgg tttgctcgcg atcgccggtc tggcgacgct
     1681 cgtcgccgcg gcgctcgtca caccgtttct cggcggaagc ggggcgctcg tcgcctcgct
     1741 cggttgggcc gtgtcgctcg tgccggcgct ggtgatggcc cgtcacacgc gctggggcgt
     1801 cctacccgtg ctgttgacgc cgttcgtctc cccggtgatg gtgtgggtcg tggccaactc
     1861 ggtctatcgc acgctgcgac gcggcggcat ctgctggcgc gggacgttct acgcgatcga
     1921 cgcgttgcgc gacgggcagg tgcgaccgtg atccgtcctt ccgttgacac cgtcgcgatc
     1981 accgatatgg tggtcgatga tcctccgcgg gtacgaggtc cggggatggg acgagcgatc
     2041 aaggtcgaag cgccgcagct ccggctcgat ccggcggcga tcgccgaatc gatcgactgg
     2101 ccgaccgtat tcggtcgcgg cggtccggtc gagatcgaga tcggcatcgg taaggggcgg
     2161 ttcctgctcg agatcgcccg gctgcgaccg gaggtgttgc acctcggcgt cgagtgggcc
     2221 aacaagtacc tgcgtgtcgc cgaacgacgc gccgcgcgcc gagcgctcga caacgtgcgc
     2281 ttcgcgcgcg tcgacgggcg cgagctgatc cgccgcgcgg tgccggacgc ttcggtgtcg
     2341 gcgtactacg tcttctatcc cgacccctgg ccgaagaagc ggcatcacaa gcgacgcttc
     2401 ttccgaccgg cgacactcga cgatctggca cgcacgctgg tcgcgggcgg cgcgttgcac
     2461 gcagcgaccg accacgccga gtactggagc gtgatcgaaa cgctgatgga cggggatgac
     2521 cgcttcgagc ggtcggccga gttcggcggc cccggctttc ccctaccggt cgacggcccg
     2581 ctgaccaact acgaggcgaa gtacggcgtc gaggggcgct cgcggaaccg cggtagctgg
     2641 cgtcgccgtt gactcgtgct cagctcttgc gagcgaggca cgcccgcacc gtcgccgtcg
     2701 atcggtggtc cgtccgacgc agacagccga ggaactcgtc acgcaccgag cggtagctct
     2761 cgccccaacg ctgctcgagc aggaccatcc gctcgaggcg cctgctccag gcttcgacga
     2821 cccccgctgc cgcggggccg tcgaacgaga actcttcgtc ccgcagccgc agagcggact
     2881 cgatccggaa gcccgccgcg cccaagtgct gcgctagctt gctccccgcc cggaagtcat
     2941 agcgtcccgc cgccagtgct tcctcgacgt aggcgtcgaa ccgtgcgcgt gccgcggtac
     3001 cgagcggctc gtggccgaac aggtcgtcca cctcgacgag catgacccac ccgccgggct
     3061 cgagattctg cgcccagcgc gacagcgtgc cggcgaggct cgggaagtac gccgcgacga
     3121 agctgcacca caagccgtcg gcgcggatgc cgaggtcggg caatgcgccg aggtcggcct
     3181 ggcggaactc cgcgttcgga atccggcgcg agcgggctac ggcgagcagc tcccggttcc
     3241 cgtcgaggcc caccacgcgc gcaccgcgcg cggcgagctc ggccgccaga tcccccacac
     3301 cacagccgag gtcgatgacg acccgctcct cgacggacgg caaggcggcg agcgccgtcg
     3361 cccaatcccg ccactggaac tgctgccggt actcttccgc gagatccacg ctcgggacta
     3421 tagcgcgcga ggggaggcgg ccgaattcca agcgcatcca tccgccggga ctggcgtacg
     3481 gcgtcgaatc gatgttaaga tccggccact caaacactta ggggagtagc tcaattaggc
     3541 agagcatcgg tctccaaaac cgaaggttgc aggttcgatt cctgtctccc ctgccagcac
     3601 ccgccgccgc cgttcgtcct tgcctgcctg cccacgcgaa gttagctttg cgccagagga
     3661 gaatcccgat gggtgtcaag acgttcgtcc gtggcgagct gatcgagatc atcgaatgga
     3721 ccgatgcgac acgcgacacg ctggcctgcc gattccccga cgaagaccgg gcgatcaagc
     3781 gcggcgcgca gttgatcgtg cgcgaatccc aggtagcgca gttcgtctac ctcgggcagt
     3841 tcggcgacac tttcggcccg ggaaagcacc ggctcgtcac cgacaacatc ccgatcctga
     3901 cccgcctcgc ctcgtggcgt tacggcctcg agtcgccgtt caaggccgac gtctacttcg
     3961 tcaacacgcg gctgttcacc ggcaacaaat ggggcacgtc gaatccgctg atgatgcgcg
     4021 atgacgatct cggcgtcgtt cgggcccggg ccttcggcac ctacgactgc cgcgtcgtcg
     4081 acgccaaacg gttcctgcgc gaggtggccg gctcggacca cgactttcgg ctcgaggagt
     4141 tcgccgagac gatgcgctcg cggctggtca gcgtcttcag cgatgcgctg gccacgtcga
     4201 gagttccgct gctcgacctg gcggcgcgtt acacggagct cggccacgcg ctgctgccgc
     4261 tgatcaaccc ggtgatggcg ggcaagtacg gcgtcgagat cgacaacttc gtcgtcgaga
     4321 acgtgtcggt gccgtccgag gtcgagcagg cgatcgacaa gcgctcgagc atggccgccg
     4381 tcggcgacat cgacgagtac gtcaagttcc agctcggccg cggtctcgag ctcggcggcg
     4441 gcggagcggc gggtatggcc agcgagatgg ccgtcagtct ggcgatcgct caagagatga
     4501 tgcagcgcca acgcagcgcg ccggccgcca ccgaagcggg agcgagcgat ccgcgctcga
     4561 tccccgattt gttgtcggtc gagcaggtag ccgagttgct cggagtcgat cgggccgacg
     4621 tgaccagcgt gatcgagtcg ggtgagctcg aggcgaagag gatcggccgg agctggcgcg
     4681 tcaagcgctc ggcgctcgaa cggtacctgg ccgattgatc acaggtcgaa cagcagacgg
     4741 atcatcccgc cgacgaggtc gtcgcggtcg aagcccgtgc gttcgacgcg gatcgacgtc
     4801 gatccctgac cggccatccg atcgaggcgc tggttgagga agcgatcggc cgccgacacg
     4861 ccgcgccccc gttcctcgtc ggcgcgtttg cgcgaacgcg cttcgccgcc ctggcggatc
     4921 caacgcagga tgcggtccag ctcttccgcg tcgaaccaga tgccgtggtc cttgcaactg
     4981 tcgatgacga tcccgctgcg ccggccgaag ttcctgcggt tcatcatctg gttgcactcc
     5041 gggcagcgcc ggtagagcga accgtgctgc gccgccggcg aagcaccccc gccagcggcc
     5101 gtcgccgcca gcggctcgtc ggccgaggcg gccacgccgc gcgccttctc cgcgatcagc
     5161 tcgaacgccc ggcgtccgac ccacatcccg gcgcacgcgc cgcactcgag caccgtgacg
     5221 cgatcctgcg cgaggcttcg gctgttcagc gtgcggccgg agccgcagac cgggcagtcg
     5281 acccccgtcg gttccccggc acgcccttgc ggcacgatcg gcgtggcgca gtgatggcag
     5341 aagcgcgcca ggttgctgat acgcgccatg cattgcgggc agatcgtctg caggtcgcgt
     5401 tcgtgcaagg tgaagtcggc accgcagtgg gtgcatgcgg cgcggttgcc ggtgcgcgga
     5461 gcgctgcacg acgagcaacg gacgacctcg gcctcgaaca ccttgcgccg cggcacgacg
     5521 accgtcgcgc cgcacgagca gtggaagcgg ctgccgggcg ccagcgcgcc cgcgtcatac
     5581 tgccgcctgc agccgtcgca ggccacgagg aacttgagcg ccgaaggcga ttcgacggcc
     5641 atggggccca atctagtcgc gccgtccggc gaagtcacgt tccgctatca tgccgcgcgg
     5701 gagaccgact cgtgtggcgt tgaagctgtt tctcaccggc gaccccggct gcgggaaaac
     5761 gaccgccgtg cgtcgcgtcg tcgagcgact gcgcggccgc gtgaggatga cggggttcat
     5821 caccgaggag ctgcgccgcg acgggcgtcg catcggcttc cgcggcgtca cgctcgacgg
     5881 gacgccgttc ccgctggccg atcgtgacgc gcggggcgaa ctgcgtgtcg gcccgtaccg
     5941 cgtcgtgctc gacgggctcg agtcgatcgg cctggccgcg ctcgagccgg gcggggccca
     6001 gctggtcgtg ctcgacgagg tgggcaagat ggagctgttg tcctcgcgtt tccgcgagcg
     6061 ggtcaccgcg ctggtcgagg gcacgactcc gctgttggcg acgatcgcgc tgcacggcgt
     6121 gggcttcatc cgccggctgc gccacgatcc gcgcgtgacg ctggtcaaga tgagccgaca
     6181 cgacagcgac accgccgtcg gcgatgcgat ccgccgactg cgcgacgcgg gagtcgtccc
     6241 gcgccgcaac ggcacgggtc ggtcgtgacg gaggcgcggg acgcggccgt ctcagcgcga
     6301 ccggtcgatc ggccggcgta tccaatgatc tgtgtagtgc gttcccgctt ggtcttccgg
     6361 ttcggcgcga cgcatgtgac acgcggtgca gtcatcgggc ggccgcgtcg ccgcgcgggt
     6421 gcccggctcg agggtgcagt cgtccgccgc gtggcaccgg aggcaggcgc ggcggaaccg
     6481 ctcggggggg cggtcgtcgt gatagacggt gacgtgcggg tcgtggcagg tcaggcagtc
     6541 gaccccgccg ccgctttcga cgtagcagcg gctcgacatc aaccgcgcgc cttgcgcgtc
     6601 ggtggcgaat ccctcgagaa acggccccgg gcggaactcg gtgacgcgcg cggcggcggc
     6661 cgcgcggtcc tcgtgcggga ggccgggagg ggcgccgaag tggcagcgca ggcagaccgc
     6721 catgcgctcg gcgtgcggta ggcgcctcgg gttgacgatc gtcgggtcgg cctcaccgtc
     6781 gctgccggtc gcgctgcgcc agcggtcgac gtggatcgag cccggtccgt gacagcgctc
     6841 gcagtcgacc ccgtcggctg cgacgccctc gcgcgccacg gcggcgtgac agccgagaca
     6901 gtgctcgagc ggtgcgcccg ccaggtgcga cggcgtgcgg ggatcgtccg gacattcgtg
     6961 ccactcgtgc cccggcgcga gcgagcacga cgccggtcgc gtcgcgtcgc cggcgtcctg
     7021 atggaagatc gcgcgggcgt ggttgcccga gccgacgacg agtcgtgccg gacccgctgg
     7081 gcgcggatcg atctccgcca tgcggaccga acgcgccatg cccgtcccgg cgtacgacgt
     7141 gtagacgccg agatggcaac cgctgcaggc ctcgatgccg acgtagtccg ccgcggcgag
     7201 gtcgacgctc gccgcgccgc gcgggtcgcc gccgccgcac gcaaccccga gcagcaccgt
     7261 acaaacgacc gccgccggcc ggccgaggcg cgaggcgagt cgcatgaatg acgcgctcac
     7321 tccggctcgg cgagaccgta gatccagcct tcgcggcgga cacgttcggc ggcgtcgggc
     7381 gcgagctgct cgggtgtgcg ctcgacctcc cccgtcatca ccgcgaccgc cgcctgccgc
     7441 gcggcgatca ccgcgtcgag cgcgtcgcct cgttcgaggc acttgcgcgc agtacgcgcg
     7501 tcgcacgtcg tcggcaggta gccggcatgg ccgagcgcgg acaggatccg ctgccggcgc
     7561 ccacggccgt tggctttttc cgacacggcc agcggcgaca ggcgccgcgt gaaccctccg
     7621 gggtagactt cgagcagcaa acgcccttgc gccgactcga acggtcggac cgcgtaacgg
     7681 gaccggtcct cggcgatcat gcggatgccg tggaaggtca tcggtcctcg atcggggtcg
     7741 acgcggtgca gcggtgagaa cgcgtccgcc acctcgtcgg tgtggcgcct ggactctcca
     7801 tgctggtcgc ggtactcctg gagcgcgacc aggtactcgg gtcgtgacag tgtctccatc
     7861 ttgcgcgcca gcgcccacca accctcgtcg ggaaatgcac cgccgagcaa gcgctcggca
     7921 aagccgaggg gaacgctgaa cgggaaatcg agaccgatcg cttcgacgtt catcaacgtc
     7981 gcgtcgggtt cgcgcaggta tccctcgaga ccgtgccgcc cggtcgcgtc gagacgaacc
     8041 acgttcagcc gatcgccgca cagctcgacg atcgcgagcc acgtcgcctc gcgcgggtcg
     8101 cgcatgtcac cgctgtaatc gacgccggcg aacagccggc cggcgggcat acggcgcccg
     8161 acgccccgac gcgggacggc cgccgcgacg gccggcgccg gttcggcagg ccgcccggct
     8221 tcgcccgcgg cctcgacgcg cgagccgctc tcgcgatcct ccggctcggg tacccagcct
     8281 tcggccagcg cggcgtccca caccttgccg aacagccgta cctcggcatc gggccagccg
     8341 ggatcgcagc agagaaagac ccgacgcggc ggctggtcga acgcgagcgg cgacgcgaac
     8401 cggtggacgg tcgagaggtc gacccaggcg tagcccttgg tctcggccag gcggacgccg
     8461 accgcgcgag acagcccgag gaacttctcc gaacgttcgg tatgtcgctg gctggccggg
     8521 tagtagatcg cgatcagatc gcccaggcgc agccgggagg cgatccgctc gaacgccagt
     8581 acgtgtcgag gcattccgtc gatccagatg ttcgggtagc gggcgcgcga gtcgttccac
     8641 tccatcacgc gcagcaacca gcgcgagcgc cgccgcagct cgtccgattc cttcacgtaa
     8701 ccctccccgg cggcagttcg agccgccgtt tccgggatga ggacctcgtt cgcgggggac
     8761 gtcaggaaag ggaccacgcg cacccgccgc tgtcaagcgc ggtggcactc ggtttgcttt
     8821 tctgtcagta tcgaaacgtc gcttcgcgcg tctcggcggc gggcggcccc gcgggccccc
     8881 gaggggcagg cccgggcgac ggggcgagac cggcggcatg gcgtcttcca tcggagttcg
     8941 tttgaacgcg gaagcgataa acccctcatt gccggggact aacgtcgatc tgccaccgac
     9001 cgtgatcctc gtcggcaacc ccaacgtcgg caagagcgtg gtcttcaagc acctcaccgg
     9061 tcgccacgtc acgatctcca actaccccgg gacgacggtc gagatcactc gtgcgccggc
     9121 gatggtcggc gggcgccgta cggagctttt tgacacaccg ggcatcaacg acctggcgca
     9181 ggagagcgaa gacgcgcagg tcacgcggga gatcatcgcc aggcacccag atgcgactat
     9241 attgcaggta gctgatgcca agaatctgag gcgagcgctg ctcctcacga tccagctcgc
     9301 cgagctcggc cggccgatgg tgctcgtcct caacatgatg gacgagctcg agcgctcggg
     9361 agcccgaatc gacacgcccg cgctcgcgcg aacgctcgga gttccggtcg tctcgacggt
     9421 cgcgatcgcc cgacacggaa tcgatcagct cgtcgcgtcg attccagacg cgaagcccgc
     9481 gggcgtcgcg gtcgtcgtcg acggtccccc gcgcgaggtc ttcgtgcgca atcagatgcg
     9541 aatggatcgc gccaacgaga tcctcgccga aagctaccgt ctcgagcgtc cggcgcgtcc
     9601 tccgttcagc gttcgtctgg gcttctgggc gatggacccg ttgaaggggc tcctcgtcct
     9661 gctcgccgcg ttgctgttca ccttctggtt cgtcggtctg atcggtgcgg gcaccttcgt
     9721 cgatctgttg gagacggggc tgttccatca gggcctcaat ccgctcgcga tccgcgccgc
     9781 cgacgcgtta ctgccgttcg accacaccca ttcgcccgaa ccggtcacgc tcgaggtctc
     9841 gatcccgctg tcgcccgcgc acgggatccc gctggcgtcg ctcgagcgcg aggtggcaac
     9901 gacggcctac gtgctcgacg gcgagccggc cgacggcccg ctcgcggcta gcgtgcgtct
     9961 cgtccacgac tttctcgtcg gcgagtacgg catgatcacg atggggttga gctacgcgct
    10021 ggccatcgtc ttgccgatcg tgatcacctt cttcctcgtg ttcagcgtgc tcgaggattc
    10081 cgggtatctc ccgcggctgt cgatcatggt caaccgcttg ttccgcctga tggggctttc
    10141 gggcaaggcc gtgctgccga tggtgttggg cctcggctgt gacacgatgg cgacgatgac
    10201 gacgcgcatt ctcgagaccc gccgcgaccg gctgatcacc tcgctgctgc tggcgctggc
    10261 catcccgtgc tcggcgcagc tcggggtgct gctggcgatg atggcccagc tgtcggtcgg
    10321 ggccgccgtg ctgtgggtcg tgctgatcgt cggcgtgatg ctgctcgtcg gctggctcag
    10381 ctcgaaggtc tttcccggcg agcgaagcga tttcatcctc gaactgccac cgatccgccg
    10441 cccgcagatc ggcaacgtcg tggccaagac gttcgcacgg atcgagtggt acctcaagga
    10501 ggtcatcccg ctgttcgtgg cgggcacggc gatcctgttc gtcctcgacc gcacgcacgt
    10561 gttgacgtgg atcgcggccg ccggcagacc gctcgtctcg ggttggctcg ggctgccggc
    10621 cgacgcgatc aacgcgtttc tcatcggctt tctaaggcgg gacttcggtg ccgtctacct
    10681 gctcgatgcc gcgaccggtt ccgacgccat cctgtcgccg ttgcagatct tcgtgtcgat
    10741 ggtcaccatc acgctgttca tgccgtgcat cgccaacctg ttcatgatcg cgcgcgagca
    10801 cggcaagcgc atcgcgctct acatgacgct cttcattttt ccgttcgcct tcctcgtcgg
    10861 cgggctggtc catctggtcg gccgagccct ggggtatcga taagtgaagc acgcgccgct
    10921 ctacctccac tgctcgttgt gcggtctcga cttcgagaag accgatacgg tgtgccagcg
    10981 cggctgtccg ctgggcgtgg cctgcggtca cgtgcgctgc ccgaactgcg ccttcgagtt
    11041 cccggagccg cggagacccg cacaatggct gcgtcgaatc ctcggccggc gcgggcgact
    11101 cgatctgcgg gcgctcgagg gcgcgaccgt cacgctcgac cggctcgact ccggcacccg
    11161 ctgccgactg cagcatctcg gttgtcggag ccacggccgg cgcaacacgc tgacggtctt
    11221 cgggatgacg cccggaaccg agatcgagct gctccagcgc cagccgagct tcgtcgtccg
    11281 ggtcggcgaa acggaggtcg gtctcgacga ggagatcgcg cgcgagatct acgtcaagcc
    11341 gatttgaccg cggccgcggt cgcgtgcgtc gcggtatcct gtcggcgggc tgaaaccgat
    11401 gtcgacccct cgtcggattc ccgctgtcca cgtggccgcc ttcatgatcc tggccgcctg
    11461 ctccgcgtcg tcggccgcgc ccgggccgct cgtcctcgtc gggggcggtc tcgacgagtc
    11521 gaacgagccg gtctatcgcg agatcctcgg cctgcgcgcc ggcccccggc cgatctgcgt
    11581 actgccgacc gcgagcgacg agccgcaaca ggcggccgac cggttcgccg ctcgattcga
    11641 gcgttacggc ggcccgggga cggcgacggt ggttcggctc aagcccggtg gcgccgacaa
    11701 gccgaagatc gcccgccggc tcgagcagtg cggcgggttc ttcttcctcg acggtgatcc
    11761 gtcgcgcatc gtcgacacgc tgcgcggagg cggccggacg agcctggccg agcaggcgat
    11821 cctgcgcgtc caccggcgcg gtggcgtgat cgccggatcg aacgcggccg ctgcggcgat
    11881 gagcgacccg atgatcgccg gcgagaccgg cagcgacgac gcgttgcggc acggggtgtc
    11941 gtcgtccgcc ggcgaagccg gcctgtgggt tcgcgacggg atggggtttc tcgacgccgg
    12001 attgaccgac ccgcatcaca tctccgcggg aaggaccggg cggctgctcg tcgcgctggc
    12061 cgcgctggac ggcgtcgatc ggggtttcgg tatcgacgac gacacggcgc tcgtcgtcga
    12121 cggaacccgc gcccgggtgg tcggctcgtc gcaggcgatc gtggtggaga aggtcagcgc
    12181 cggcagcggg cggctgcgat tggccggtgc gcgctttcgc gtgttggtgc tcggcaacgg
    12241 cgacggctac cacctcgacg acaggacggc gttcgtcgac gcgggcaaga acgtgctgcg
    12301 cttgcccgac cgggtgccga cgccgcccga cggtccgtgg ctggcgagcg ggctgccgct
    12361 gttcctcgtc gagctgtcgg tttcgagcgc cagcgaggcg accttcgagt cgcgcgggtt
    12421 ccgcttccgc ctcagcaagg aagccggttt cgaggcgcgg gcctgggagc tgccggacgg
    12481 acgccatcta cctcacggtt tcaccgccgg ttggttcctc ctcgacgtca gcgaagcgac
    12541 cccgtgagcg ggtcggccgg cggattttgc ggcgtcggat cgtctcgttt gaccggcgta
    12601 tcgattccga agtcggtgca tctctgacga gagagccgac ggcggcgagg aacgacgatg
    12661 tggcgatgct gtctgccgat cctggtctcg ttgtcgatcc cgctcgggtc ggccgcgggt
    12721 gacgaaccgg gcgagccgat cggacccgcc gatgcggtgc gcctcgcgct cgagcacaat
    12781 ccccgcctgc tggccgccga cgcggggctg agggcggccg aggccgatcg gcgtgccgcc
    12841 tcggcgagcg gtctgccgcg gatcgagttc accgaggact gggtgcggtc gaacaacccg
    12901 gtcttcgtct tttcatcgaa gctgctgcag ggggtcttcg cgacggacga cttcgcgatc
    12961 gacgtactga acgaaccgga gccgatcacc aactccaccg cgcggctgtc gatctaccag
    13021 agtatttgga acgggcgcaa ttcgccggag aagaagacgg cggtcgccgg ggtcgcggtc
    13081 gccgaatcgt cgcagcgcaa ggcgcgcgac gaggtggtgt tcactacgct cgaagcgtac
    13141 tgggcgctgg cgtcggcgcg acggatgatg gaggtgaccg cagacggcgt cgcggcggcg
    13201 gaggccaacc tggcgctggc gcgcgagctg gtcgacgccg gactggcggt gccgtcggat
    13261 cgcctggcgg ccgaggtgcg gctggcggac gttcgggcga cgagcgtccg cgcggagtac
    13321 ggcgtcgagg tcgcgcacgc ggccctgctg cgcgcgctcg gcgaggaccg cccggagatg
    13381 cgttacgtgc cgatcgagac cgaccggttg ccggagctcg gcgacgattc gctcgaacga
    13441 cgcaccgagc aggcgatgct cggccgggcc gatctcgccg agctcgaccg gcggatcgaa
    13501 caggcccgca tcggcgcgcg gagcgcgcgc gccggtcgct accccgagat cggcgcggcc
    13561 ggcagctacg agtggaacgg cgaacgcctg ctcgccgccg acggcgagaa ctgggccgtc
    13621 ggtctggcgc tgcgcatgac gatcttcgac ggccaccgga cagacgcgcg cgtgcaacgc
    13681 gcggcggccg agctggcgca gctcgaggcg atgcgggtcg cgctcgcgca gggagtacgg
    13741 ctcgaggtcg catcggcgtg ggccgagcgc gaatcggcgg cgcgccggct cgagttggcc
    13801 gccaccgccc tcgagcaagc cggcgaggtg atgaggatcg tgcgcgaacg gtacggcgaa
    13861 ggggtggcgt tggtcgtcga actgttgagc gcagaggcgg cgttgaccgc ggcgcaggcg
    13921 cagcaggtgg tcgcggtcgg cgagctgtgg ttggccgacg ccggcctcga cctggcctcc
    13981 ggaaccgacc cgatccccga tctgccgtcc gtcaccacgg gcggtccggg ttgatccgat
    14041 ggagcgcgcg atgaagacga gttcactgcg gtcggtcgta atgctgctgt cgctcgtgct
    14101 cggcggttgc gccggtgatg gcggccgtcc accggccgag ccccgtccgc gggtcgcggt
    14161 cgacgtggaa cgggttgccg tcgagtcgtg gcgtgacggc ctcgagctga cggccggcgt
    14221 gttgcccgcg gttcgtgcga cgccgggcac ggtgctgatg ggtcgtgtcg atcgcgtgct
    14281 ggtcgaagaa ggcgacgtcg tcgagcgcgg ccagacgctg gcacggatcg agagccgcga
    14341 cgtcgcggcg cggttggccc aggcgcgagc gggggtggcc gcggcccgcg ccgccgagca
    14401 gaacgcgcgg cggatgcacg agcgcatgga gcggctgcat gccaacagcg cggcgagcga
    14461 caagaacctc gaggatgccg aggcggcgca cgaggcggcg ctggcgcagc tcgcggccgc
    14521 cgaggaggcg gtcaaggtgg ccgaggtcca ggtcgactat tcggagatcg tcgcgccgtt
    14581 cgccggcgtc gtggtggaca aggggatcga ggccggcgac acggccgcgc ccggcatgcc
    14641 gctgttcgtc atcgacgatc tgtccaaggt caagatcgag gtgcaggtgc cggagtcggc
    14701 cgccgcgtcg gtcgcggtcg gtgacccggt cgaggtcgag ttccggggcg agcggttcga
    14761 cgggcgtttg tccgagctcg tgcccgcggc ggacccgcgc tcgcgcaccg tcaccatgcg
    14821 tgcgctgctc gacaacccgg aacggcggct gcgcgcgggc atgttcgcgc ggctcgtgct
    14881 gcccggcggc gtgcgcgatg ccctgacggt tccgtcgagc gcgatcgtcc gcaagggacc
    14941 actgaccggt gtgttcgtcg tcggctccga tgcgagcgac cggccggtgg ccgggctacg
    15001 ctgggtcacg ctgggcaccg agcgcgccgg ccgggtcgaa gtgctgaccg gtctcgacgc
    15061 gggcgaacgc gtggtctccg cgccggacgg caggctcgag gatggtcaac cggtcgaggt
    15121 cggcgaatga ccggcatcgg catctcgggc cggatcgcgg gagcattcat cggcaacaaa
    15181 ctgactccgc tgttggcgat cgcggcgctg gtgctcgggc tgatggccac cctcgccaca
    15241 ccacgcgagg aggagccgca gatcgtggtg ccgatgatcg acgtgatgat cggctggccc
    15301 ggggcgagcg ccgaggacgt cgagcgcttg gtcaccgtgc cgctcgaacg cgcgctgcgc
    15361 gaaattcccg aggtgcgcta cgtctacgcc acgtcgcgac cgtcggcggc gatgctgatc
    15421 gcccagttct acgtcaactc cgacccggag cgggcgctga tcaacgtgcg cgacaaggcg
    15481 gccaacgcgg gcgggcgctt gcccgccggc gtcgcgccgg ccacgatcac ctcgcgttcg
    15541 atcgatgacg tgccgatcct cgcgctcacg ctggcatcgg gtcgttacga cccgctgacc
    15601 ctccgacgga tcgccgccga gatcgaggaa gacgtacggc agatccccgg cgtgtcgacg
    15661 acgacgttga tcggcggcct cgggcgcgag atccgcgtcg agctggacct cgagcgattg
    15721 gccgcgcgcg gtctcgcgcc ggccgtcgtg ctggacgcgc tcgagcggag cggggtcgcg
    15781 ttgccggcgg gaagcgtcgg gtcggcgcgc ggagagctgc tgatcgaaac cggcgagttg
    15841 ctgcgctcgg cggccgaggt cggagcgacg gtcctcgctt cgcccgacgg cagcccggtc
    15901 cacctgcgcg acgtggcggc gattcacgac ggggccgccg agccgcggct gtacgtgatg
    15961 cacgccgagc acggccgccc gtcgcagccg gcggtcacgc tgtcggtggc caagatcgaa
    16021 ggggccaacg cgaccgacgt ggcgcaagcc gtcctgcagc gcgtcgagcg gatgcgcggg
    16081 cgaatcgtgc ccgaggacgt gacgctgacc gtcacgcgcg attacggcga aacggccaag
    16141 caaaaatcgg acgagctgat cctgcacctg atcgtcgcga ccgcgtcggt cgtgttgttg
    16201 atggcggtct ctctcggagt ccgtgccggc ctgatcgtcg gcgtcgcggt gccggtcacg
    16261 ctgtcattga cgctgctgat ctactacctg tggggctaca cgctcaaccg ggtgacgctg
    16321 ttcgccttga tcttctcgat cgggatcctg gtcgacgacg cgatcgtggt ggtcgagaac
    16381 atccttcgtc acctgcggat gaagaagctc ccgccgctgc aggccgcggt gttcgcggtg
    16441 gacgaggtcg gaaacccgac gattctcgcg accttcaccg tgatcgcggc gatcctgccg
    16501 atggcgttcg tctcggggct gatgggaccc tacatgcggc cgatcccggt cggcgcctcg
    16561 ttcgccatgc tgatctcgct gctggtggcg ttcaccgtct cgccgtggat cgccttgcgc
    16621 gtgctgcggc gcgacgacgt ccaggcgggt cgcgcttccg acgcggcggc ggccgaaggc
    16681 tggcacgcgc gggcctaccg tcggctgatg cggccgctga tcgagcgccc gctctaccgc
    16741 tgggtgatct tcgccggcgt cgtggtgctg ttgctcggtg tcgttgcgct cgtgccgttg
    16801 aaggtggtca ccgtcaagat gctgccgttc gacaacaaga gcgagctgca gctgatcgtc
    16861 gacgctcgcg aagggacgac gctcgaggac acgttggccg cggcgcgcgc gatggccgac
    16921 cggctcgccg tcgagcccga ggtccgcgac atccagatct acgccggcac gtcggcgccg
    16981 ttcaacttca acggcctggt gcgccactat tttctgcgtt ccggcgccaa cgtcgccgac
    17041 ctgcagatca acctgatgcc caaggaacag cgccgcctct cctcgcacga gatggcgagg
    17101 aaggtacgtc cgctcgtcgt cgaaatcgcg cggcgccacg gcgttcgcgt caaggtggcg
    17161 gagatcccgc ccggtccgcc cgtgctgtcg acgctggtcg ccgaggtcta cgcctccgat
    17221 cgaccgacgc agatcgcgat cgccgaacgc gtgctcgcca tcttcgaatc caccgacggc
    17281 gtcgtcgaca gcgactggta cgtcgaggcc gaccagccca agctgcgttt cgtcgtcgat
    17341 cgggaaaagg cggcgctggc cggcgtcgac cccgagcaga tcgcgatcac gctgcgtctg
    17401 gcggtcgatg gcatcgacgc gacgatggcc caccccggcg acgaacgcga accggtgccg
    17461 atccgcgtca ccctgccgcg cgccgcgcgc gcgcgggacg aggtgttgcg cgacatccgg
    17521 ctgatcgccg ccgacggttc ggtcgttccg ctgtccgagg tggcacgcgt cgagtcgacg
    17581 acggcgcgcc cttatctcta ccgcaagaac ggacgggcgg tgacctacgt gatcggagac
    17641 gtcgccggaa gcgaggagtc gccggtctac gccatcctcg ccatgtccga ccgcatccgg
    17701 cagatcgagc tctccgcgtt gggcgcgatc gacgaacgga tggtcgacga cccgctggcc
    17761 ggcgatcgcg aggcggtggt gtgggacggc gagtggcaga tcacctacga ggtctttcgc
    17821 gacctcgggg tggcgttcgc ggccgtgctg gtgctgatct acgtgctggt ggtcgcctgg
    17881 ttccagtcgt tcaccgtgcc cttcgtgatc atggcgccta tcccgctcac gctcgtcggg
    17941 attctgccgg cacatgcagc gaccggagcg ttcttcaccg cgacgtcgat gatcggcatg
    18001 atcgcgctcg caggaatcat cgtccgcaac tcgatcctcc tcgtcgactt catcacgctg
    18061 ggtctggagc gcggccagcc tctccgcgat gcggtcgtcg cct
//
LOCUS       DASPGN010000372         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_302507, whole genome shotgun sequence.
ACCESSION   DASPGN010000372 DASPGN010000000
VERSION     DASPGN010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302507"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VD788_10705"
     CDS             complement(<1..1472)
                     /locus_tag="VD788_10705"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76777.1"
                     /translation="MRKASFRSRRPVATGLAGPGVQGRLCALLFAAFAALVPASPARA
                     YDAMVAWEPVAGAVGYLLYVAYGDDPFGSAIDLGAADAQVAGKLRAWVEGLPLGPTAR
                     FAIAAYSYPGVVGEQSNALELTYPRVAEVVDSDGDGLKDAQEDLDLDGVVDPGETDQR
                     NPDTDGDGLSDYEERAIWRTDPVDADSDGDGTRDGDEVALGTDPNDPTVGGGMPCNPS
                     SGNCPGQDAEVWIAAATYPAVLLEGAMSTGLRFTHGSDADVHRDSLAPWVIFPDSSTS
                     AFAAGSEDRAVYQIAVPVSGSWYLWGRFYYPSDGGDNGANSFFATVDGGRPQRFGNNL
                     GYFQRWHWDGDGNVEDGARSPLLLGFLEAGVHQLTIEKREVNPIPPRLDALFLTTDPT
                     AVPDDATARTRLDVCPGGRCWRCGDATGNRRITVDDAWLILSAAVNPGLCSLSVCDVD
                     HDGHVSATDAAVLLQTVIAGGADDLVCDGAFTVGVSGAIAL"
     gene            complement(1725..>2141)
                     /locus_tag="VD788_10710"
     CDS             complement(1725..>2141)
                     /locus_tag="VD788_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76778.1"
                     /translation="RRYALPQLRRLGAVVGRFMRSDSKLSVVQSSLLAFLVAAAILSL
                     SGCRTSATGGDDPAPEPGAGATRSEDGAAEQQPAAGAGMQVHIDPETGEFLDEPPPGT
                     ELGGDVPTPPSGAVERPAPGGGVMIELQPKSPPATE"
BASE COUNT          349 a          740 c          718 g          334 t
ORIGIN      
        1 agcgcgatcg caccggagac gccgaccgtg aacgcgccgt cgcagaccag gtcgtcggca
       61 cctccggcga tcaccgtctg cagcaagacc gccgcatcgg tggcggagac gtgcccgtcg
      121 tggtcgacgt cgcagacaga caggctgcac agccccgggt tgacggccgc gctcaggatc
      181 agccaggcat catcgacggt gatgcgccga ttgccggtgg cgtcaccaca cctccagcag
      241 cgtcctccgg ggcagacgtc gagacgggtg cgcgcggtgg cgtcgtcggg gaccgcagtc
      301 gggtcggtcg tcaaaaacag cgcgtcgaga cgcggcggga tcgggttcac ctcgcgcttc
      361 tcgatcgtca gctggtgaac gccggcctcc aagaacccta gcagcaaggg cgagcgggcg
      421 ccgtcttcca cgttgccgtc gccgtcccag tgccagcgct ggaagtatcc gaggttgttg
      481 ccgaagcgtt gcgggcgacc gccgtccacg gtggcgaaga acgagttcgc cccattgtca
      541 ccgccatcgc tcggatagta gaagcggccc cacaagtacc aggagccgga gaccggcacg
      601 gcgatctggt agaccgcgcg atcctcgctg ccggctgcga acgcgctcgt cgagctgtcg
      661 ggaaagatga cccaaggagc caggctgtct cgatggacgt cggcatcgct gccgtgggtg
      721 aaccttagcc cggtgctcat tgcgccctcg agcagcaccg cgggataggt ggccgccgct
      781 atccacacct cggcgtcctg acccggacag ttgccggacg acgggttgca tggcatgccg
      841 ccgccgaccg tggggtcgtt ggggtcggtg ccgagcgcga cctcgtcgcc gtcgcgcgtg
      901 ccgtcgccgt cgctgtcggc gtcgaccgga tctgtgcgcc agatggcccg ctcctcgtaa
      961 tcgctcaagc cgtcgccgtc ggtgtcgggg ttgcgttggt cggtctcgcc cgggtcgacg
     1021 acaccgtcga ggtcgagatc ttcctgcgcg tccttcaggc cgtcgccgtc gctgtcgacg
     1081 acctccgcga cccgcgggta ggtgagctca agtgcgttcg actgctcacc caccacaccg
     1141 ggatacgagt aggcagcgat cgcgaaacgg gcggtcggac cgagcggcag accctccacc
     1201 caagcacgca gcttgccggc gacttgcgcg tcggcggcac cgagatcgat cgccgagccg
     1261 aaggggtcgt cgccataggc tacgtagaga agatagccga cggcccctgc gaccggctcc
     1321 caggcgacca tcgcgtcgta ggcacgagcc ggcgacgccg gcaccagggc ggcgaacgcc
     1381 gcgaacagca gcgcgcacag gcgtccttgg acgcccggac cggccagacc ggtagcaacc
     1441 ggccgccgtg agcgaaagct agctttgcgc aacgtcgagc cttttcgaac tttttctgaa
     1501 cgagaccggg cagtcgctga cggtcgtcgt cgagtgtcgt agcggctgcg ggccggggcg
     1561 gcccgcactt gcctcacctc accacatcca accgtgcccg gtcacaagag ggcggaagtc
     1621 acggagcaag ctaccgtgga ctttttcaaa gtccagtttg cgtagcgggt tatgcacaat
     1681 ccgctcgagc gattctttca atttctgtta aggacttttg cgcgtcactc ggtggccggc
     1741 ggcgacttcg gctgaagctc gatcatcacc cccccacccg gggctgggcg ctccaccgcg
     1801 ccgctcggcg gcgtcggcac gtcaccaccg agctcggtcc ccggcggtgg ctcgtcgaga
     1861 aactctccgg tctccggatc gatgtggacc tgcatgccag cgccagcggc cggctgctgc
     1921 tcggcggcgc catcctcgct ccgggtcgcg cctgcacccg gctcgggcgc tggatcgtcg
     1981 ccgccggtcg ccgaggtgcg gcagcccgac aggctgagga tcgccgcggc aaccaggaac
     2041 gccagcaatg aggactgcac caccgacagc ttcgaatcgc tccgcatgaa cctgcctacc
     2101 acagcgccaa ggcgcctcaa ctggggtagt gcatagcgcc t
//
LOCUS       DASPGN010000373         3230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_184112, whole genome shotgun sequence.
ACCESSION   DASPGN010000373 DASPGN010000000
VERSION     DASPGN010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184112"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3230
                     /locus_tag="VD788_10715"
     CDS             <1..>3230
                     /locus_tag="VD788_10715"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HXV76779.1"
                     /translation="PLLPDSDTAIGLSEPGTYDNELLDAHFMAGDGRVNENIALTAVH
                     HVFHSEHNRLVGHIKEVLVASAATGELDFLNEWLLVDVTTPPTPADIPDLVWDGVRLF
                     QAARFGTEMQYQHLVFEEFARKISPQIDIFLQEGQGYDSTIDASIVAEFAHVVYRFGH
                     SMLLDEVDRFDPNFATDNSLTLIEAFLNPTAFDLSGTLSAEEAAGAIVRGTTRQVGNE
                     IDEFKTEALRNNLLGLPLDLAAINLTRARETGIPSLNAARATFFLWTGDSQLKPYTSW
                     VDFMQNLRHPESLINFIAAYGTHPLIATEGTLAGKRAAATLIVLGGDGEPADRLDFLY
                     GTGTWANGAGRPLDLDGVTTTGLGNVDFWIGGLAEHIMPFGGLLGSTFNYVFENQLEK
                     LQNGDRFYYLERVAALNFLTELEGNSFAKLIMANTDATHLPGDVFSTPAFILEVDQSR
                     QFTGLGVDGRDDPIDDANPLNPLVIRTNPTVVDNPLTPFDEATANYLEYTGVDHVVLG
                     GTDDDDILTASIGDDTIWGDGGNDRIEGGDGVDQLRGGAGDDIITDTGGDDNLQGGDG
                     NDAIHGGNGIDLIIGGFGNDFIVTGEDGDESFGGPGNDFFLGDPADEMVFGNEGDDWI
                     EGGMADGSAGENFDARGLDAIVGNDVFIDSIFPDRMNGEGGDDIMVGSMGGQVDRFLG
                     GSGFDWASFQGDEFAADVDLSLRAFDETPVPFSIASALSRFESTEGLSGSSRSDILRG
                     DDEDITTIPFSGTQGSTLTNFALIDGLQGLVDGLLGPGQTSFAAGNIILGGAGSDIIE
                     GRGGDDLIDGDHWLSVRISVRENADGTGAEIDTVTSLTQLVDRVFSGEINPGQLVIVR
                     EILVDDGADLDFDTAHFSGALIDAGADGVLGTADDVQQFTFAINGVATALDDLAANYQ
                     DGDVLTVTNLIDDDGDGVAETPGPEGSDALRHIERLQFADRTIVLRDGLNAEPVGQLA
                     ILDSNGQPVGTPVEGQTLTVSVAGVTDADNVSSGGTVLPPIAYVWQVELRPGTGVFED
                     IIVATGLGDVRAAGTTFTVTNDVAGLAIRVRALYEDEHGVLETVFSA"
BASE COUNT          560 a         1156 c         1008 g          506 t
ORIGIN      
        1 ccgctgctgc ccgattccga tactgcgatc ggactctccg agcccggcac ctacgacaac
       61 gagctgctcg atgcgcactt catggcgggc gacgggcgag tcaacgagaa catcgcgctc
      121 accgccgtcc accacgtgtt ccactccgag cacaaccgtc tggtcggcca catcaaggag
      181 gtgctcgtcg cctcggcggc caccggtgag ctggacttcc tcaacgaatg gctgctcgtg
      241 gatgtcacga cgcctcccac gccggccgac atccccgacc tggtgtggga tggcgtgcgc
      301 cttttccagg ccgcacgctt cggcaccgag atgcagtacc agcacctcgt attcgaggag
      361 ttcgcgcgca agatctcccc gcagatcgac attttcctgc aggaggggca gggctacgat
      421 tccaccatcg atgcgtcgat cgtcgccgag ttcgcgcacg tcgtctaccg tttcggtcac
      481 tcgatgctgc tcgatgaggt cgaccgcttc gatccgaact tcgccaccga caacagcctc
      541 actctcatcg aggcgttcct caatcccacc gcgttcgacc tcagcggcac gctcagtgcc
      601 gaggaggcgg cgggtgcaat cgtgcgcggc accacgcgcc aggtcggcaa cgagatcgac
      661 gagttcaaga cggaggcgct gcgcaacaac ctgctcggcc tgccgctcga cctcgccgcc
      721 atcaacctga cgcgtgcccg cgagacgggc attccgagcc tcaacgccgc ccgcgccacg
      781 tttttcctgt ggaccggcga cagccagctc aagccgtata cgagctgggt cgacttcatg
      841 cagaacctgc ggcaccccga gtcgctgatc aacttcattg ccgcctacgg cacccacccg
      901 ctgatcgcca ccgagggcac cctcgcgggc aagcgtgcgg ccgccaccct catcgtcctg
      961 ggcggcgacg gagagcccgc ggaccggctc gacttcctct acggcaccgg cacctgggcc
     1021 aacggcgccg gcaggccgct cgacctcgac ggggtcacca ccaccggcct tggcaacgtc
     1081 gacttctgga tcggcggcct cgccgagcac atcatgccgt tcggcggcct gctcggctcc
     1141 actttcaact acgtgttcga gaaccagctc gagaagctgc agaacggcga tcgcttctac
     1201 tacctggagc gcgtggccgc cctcaacttc ctcaccgagc tcgagggcaa ctcgttcgcc
     1261 aagctgatca tggcgaacac cgacgccacc cacctgccgg gcgacgtgtt ctcgacgccg
     1321 gccttcatcc tcgaggtgga ccagagccgg cagttcaccg gcctcggcgt cgacgggcgc
     1381 gacgatccga tcgacgacgc gaacccgctc aatccgctgg tgatccgcac caacccgacc
     1441 gtcgtcgaca acccgctgac gccgttcgac gaagcgacag cgaactacct tgaatacacc
     1501 ggcgtcgacc acgtggtgct cggcggaacc gacgacgacg acatcctgac cgccagcatc
     1561 ggcgacgaca ccatctgggg cgacggcggc aacgaccgca tcgagggcgg cgacggcgtc
     1621 gaccagctcc gcggcggcgc cggcgacgac atcatcaccg acaccggcgg cgatgacaac
     1681 ctgcagggcg gcgacggcaa cgacgccatc cacggcggca acggcatcga cctcatcatc
     1741 ggcggcttcg gcaacgactt catcgtcaca ggcgaggacg gcgatgagtc cttcggcggt
     1801 cccggcaacg atttcttcct gggcgacccg gccgacgaga tggtgttcgg caacgagggc
     1861 gacgactgga tcgaaggcgg catggccgac ggcagcgccg gcgagaactt cgacgcccgc
     1921 ggactcgacg ccatcgtcgg caacgatgtg ttcatcgact cgatcttccc cgaccgcatg
     1981 aacggcgagg gcggcgatga catcatggtc ggcagcatgg gcggccaggt ggaccgcttc
     2041 ctgggcggct ccggcttcga ctgggcgagc ttccagggcg acgagttcgc cgccgacgtg
     2101 gacctcagcc tcagggcgtt cgacgagacg ccggtgcctt tctcgatcgc atcggccctg
     2161 tcgcggttcg aatcgacgga aggcctgtcg gggtcctctc gcagcgatat cctgcgcggg
     2221 gacgatgaag acattacgac catcccgttc tcgggcaccc agggcagcac gctcaccaac
     2281 ttcgccctca tcgacggttt gcaggggctg gtcgacggcc tgctgggccc cggccagacc
     2341 tcgtttgccg ccggcaacat catccttggc ggcgccggca gcgacatcat cgagggccgc
     2401 ggcggcgatg acctcatcga cggcgaccac tggctcagcg tccgcatcag cgtgcgcgag
     2461 aacgccgacg gcaccggtgc cgagatcgat actgtcacca gcctgacgca gctggtggac
     2521 cgtgtgttct cgggcgagat caaccccggt cagctcgtga tcgtgcgcga gatcctcgtc
     2581 gacgacggcg ccgacctcga cttcgacacg gcgcacttct cgggcgctct catcgacgcg
     2641 ggtgccgacg gcgttcttgg aaccgcagac gacgttcagc aattcacctt cgccatcaac
     2701 ggcgtcgcga ccgcccttga tgacctcgct gccaactacc aggacggcga tgtgctgacc
     2761 gtcaccaatc tcatcgacga cgatggcgat ggggtcgccg agaccccagg cccggagggc
     2821 agcgatgcgc tgcggcacat cgagcggctg cagttcgccg atcggacgat cgtgctgcgg
     2881 gacggcctca acgccgagcc ggttggccag ctcgccatcc tcgactcgaa tgggcagccg
     2941 gtcggcacgc cggtggaggg gcagaccctc accgtatcag tcgccggcgt caccgatgcc
     3001 gacaacgtca gctccggcgg caccgtcctg ccgcccatcg cctacgtttg gcaggtcgag
     3061 ttgcggccgg gcaccggcgt cttcgaggac atcatcgtcg ccacggggct cggcgatgtc
     3121 cgcgccgcgg gcacgacctt cacggtcacg aacgacgtgg ccgggctggc aatccgcgtc
     3181 agggccctct acgaggacga gcacggcgtg ctggagacgg tgttctccgc
//
LOCUS       DASPGN010000374         2429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1003268, whole genome shotgun sequence.
ACCESSION   DASPGN010000374 DASPGN010000000
VERSION     DASPGN010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003268"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VD788_10720"
     CDS             complement(<1..578)
                     /locus_tag="VD788_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76780.1"
                     /translation="MKISRRSFLEQSARTWVALHAGAWAAAAGERPVEQPVEQPMKLR
                     ELRLAAARPAELVPFYRDALGFEMLEGRDDAVAFRAGHTRLRFRRTEVTPAPYYHVAF
                     NIPENKIERAVEWAGRRFPLIHLAGVGEPIVYFDFIDAHSIYFWDPAGNLLEFIARHE
                     LANAADGPFDASDILASSEIGIVVDDVPATVER"
     gene            661..1203
                     /locus_tag="VD788_10725"
     CDS             661..1203
                     /locus_tag="VD788_10725"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV76781.1"
                     /translation="MRNGARVTVLLLLLAPLSACRQAQEVCVLETSMGTMVFRFFEGG
                     APETTQRIKDLVSAGFYDGLPFYRVVAGHVIQAGDGGDNDRPTVVGEFGVHPHVLGAV
                     GLARDEDPDSGNTEIYICLAERPHLDGRYAVFGLLVEGFDVLERIGRVEVDEQWDGEI
                     AFHRPKTPVIIERAFLERRR"
     gene            1200..>2429
                     /locus_tag="VD788_10730"
     CDS             1200..>2429
                     /locus_tag="VD788_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV76782.1"
                     /translation="MRRLRTAISVGLLAGAVQAETVLIENVRIFNGVDDRLEPGHVLV
                     VDGLIDTVSRDRIDSPAGARVIEGGNRVLSPGFIDLHTHLTGQNPRSMERFHPTVAGA
                     TAAAMARFYLDAGFTTIRDAGGTHPDFARAIEDGLIYGPRVFPSGAIVSQTAGHGDAR
                     APHDPHPTLVGVSPYSADGSTILADGVDQTLIAVRENLKAGATQIKIMGGGGVMSEFD
                     PIHSLQPSPDEIRAAVQAAGDWGTYVLAHAYTSEAVRRLVENGVRCIEHGLLIDDETA
                     KLVADRDVVISTQLVVYRTLHELPGLSERNLKKLEVVLEGQENLIRLIKKYEIKTGFA
                     TDFIQGGYPMLTREFTERARYWTPAEVLIQATSESAEVIRMVGKLNRWGNFGEIREGW
                     VADLLLINGEPLDDISVL"
BASE COUNT          401 a          806 c          834 g          388 t
ORIGIN      
        1 cgctcgacgg tggccggcac gtcgtcgacg acgatgccga tctcgctcga ggccaggatg
       61 tccgaggcgt cgaacggtcc gtcggcggca ttggccagct cgtgacgtgc gatgaactcg
      121 agcaggttgc ccgcgggatc ccagaagtag atcgagtgcg cgtcgatgaa gtcgaagtag
      181 acgatcggct cgccgacgcc ggcgaggtgg atcagcggga agcgccggcc ggcccactcg
      241 acggcccgct cgatcttgtt ctccgggatg ttgaaggcaa cgtggtagta cggcgcggga
      301 gtgacctcgg tgcgccggaa ccgcaaacgg gtgtggccgg cgcggaaggc caccgcgtcg
      361 tcgcgccctt ccaacatttc gaagccgagc gcgtcgcggt agaacggcac gagctcggcc
      421 ggtcgcgcgg cagccagacg cagctcgcgc agcttcatcg gctgctcgac cggctgctcg
      481 accggacgct cgccggccgc cgcggcccag gcgcccgcgt gcagggcgac ccacgtccgg
      541 gcgctctgct cgaggaaact ccgtcgcgag atcttcatgg tcgtccgtag atccatccta
      601 acctgcgacg ggctggtcta caatcgggtg gccggcggtg cggaacgggg gacggaaacg
      661 atgcgcaatg gggcccgggt caccgtgctg ctgctgctgt tggcgccgct gtcggcttgc
      721 cgtcaagccc aggaagtgtg cgtgctcgag accagcatgg gcacgatggt cttccggttc
      781 ttcgagggcg gcgcgccgga gacgacgcag cggatcaaag acctggtcag cgcaggattc
      841 tacgacgggc tgccgttcta ccgcgtcgtc gccggtcacg tgatccaggc cggagacggc
      901 ggcgacaacg atcggccgac ggtcgtcgga gagttcggtg tccaccccca cgtcctgggt
      961 gccgtcggtc tcgcgcgtga cgaggatccg gacagcggta acaccgagat ctacatctgt
     1021 ctcgccgagc gacctcacct cgacggacgc tacgcggtct tcggcttgct ggtcgagggc
     1081 ttcgacgtcc tcgaacgcat cggccgcgtc gaggtcgacg agcagtggga cggcgagatc
     1141 gcgttccacc gtccgaagac gccggtgatc atcgaacgcg cgttcctcga gagaaggcga
     1201 tgagacgact ccggaccgcg atctccgtcg ggttgctcgc cggcgccgtc caggcagaga
     1261 cggtgctgat cgagaacgtc cgcatcttca acggcgtcga cgaccggctc gagccgggcc
     1321 acgtcctcgt cgtcgacggt ctgatcgaca ccgtgtcgcg cgatcggatc gattcgcccg
     1381 ccggagcacg cgtgatcgaa ggcggcaatc gcgtgctgtc gccgggcttc atcgacctcc
     1441 atacccacct gaccggccag aacccgagga gcatggagcg ctttcacccg acggtggccg
     1501 gcgcgaccgc ggcggcgatg gcgcggttct acctcgacgc cggcttcacc acgatccgcg
     1561 atgcgggcgg tacgcacccg gacttcgccc gcgcgatcga ggacggtctg atctacgggc
     1621 cgcgcgtctt cccgtccggt gccatcgtgt cgcagacggc gggtcatggc gacgctcgcg
     1681 cgccgcacga tccccacccg acgctggtcg gcgtttcacc ctattccgcc gacggctcga
     1741 cgatcctggc cgacggtgtg gatcagacgt tgatcgccgt ccgcgagaac ctgaaggccg
     1801 gcgcgaccca gatcaagatc atgggcggcg gcggcgtgat gtcggagttc gatccgatcc
     1861 actcgttgca accctcaccg gacgagatcc gcgccgcggt gcaggcggcc ggcgactggg
     1921 gcacctacgt gctggcgcac gcctacacca gcgaggccgt tcgccggctc gtcgagaacg
     1981 gcgtgcgctg tatcgagcac ggtctgttga tcgacgacga gaccgccaag ctggtcgccg
     2041 accgcgacgt cgtcatctcg acgcagctcg tcgtctatcg cacgctccac gagcttcccg
     2101 gtctgagtga acggaacctg aagaagctgg aagtcgtgct cgaggggcag gagaacctga
     2161 tccgcttgat caagaagtac gagatcaaga ccgggttcgc gaccgacttc atccagggcg
     2221 gctatccgat gctgacccgc gaattcaccg aacgcgcccg ctactggacc ccggctgagg
     2281 tgctgatcca ggcgacctcg gagagcgccg aggtgattcg catggtcggc aagctcaacc
     2341 gctggggcaa cttcggcgag atccgcgagg gctgggtcgc cgacctgctg ctgatcaacg
     2401 gcgagccgct cgacgacatc tcggtcctg
//
LOCUS       DASPGN010000375        16284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1706997, whole genome shotgun sequence.
ACCESSION   DASPGN010000375 DASPGN010000000
VERSION     DASPGN010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 16284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16284
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1706997"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VD788_10735"
     CDS             <1..398
                     /locus_tag="VD788_10735"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXV76783.1"
                     /translation="LVALLIDLEPVPAAPHAAVSSGMAAEPAAAPLTAAPEPEPTAPV
                     AEPISFDDPYVASALCTTCNECTNLNPRMFVYNANRQATIADPSAGTFEELVKAAEKC
                     PARCIHPGTPRDGDPTVDERLLARAKAFA"
     gene            412..1233
                     /locus_tag="VD788_10740"
     CDS             412..1233
                     /locus_tag="VD788_10740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HXV76784.1"
                     /translation="MSEVLAAASIMLGLAGFFGVVLSAANRYLRVEEDPRIDQVEEML
                     PGTNCGACGQPGCRGFAEALIEGRTVPGKCSVSPAAEVARIASFLGVEAGVEERRVAR
                     LHCAGGRSSVRRLADYAGIDSCRAAVLVNGGGKACPWGCLGLGDCERVCGFDAIRMTT
                     EDLPRVSVLACTACGDCVEVCPLDLFTLERVSHRVIVQCRSPLGGELARSLCRVACDA
                     CGRCALDAQQGVIEMQEGLPRLLDPARADERCTLHCPTGAIRWVVGDQFAENDRV"
     gene            1226..5335
                     /locus_tag="VD788_10745"
     CDS             1226..5335
                     /locus_tag="VD788_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXV76785.1"
                     /translation="MSEAERRGWRWLDALAGRRRERRPSFPLDLWEASLSSGLEAVLA
                     AERGAGSVVRQAIDGDGAAAVSESYGAAISGRRATAVVSDDDLGSVIDRLRTAAARHA
                     PLVIYVVARETGTAERLSSSGRTLAIAQDPQHAIDLGLIARFVAERALAPVAVVIEAE
                     GVADAVGRWCRPPAALVEHYLGRADDRIPTPTPAQATLFGERRRRVPRWFDPARPTAH
                     GMRPRGPARTAAEIGREVFFASSVPVILDQAIETLAGLTGRDAGRVRRFGPADARHVL
                     LLHGSAVPIAAATVLRGEAAIRRTAVVAIDWWTTEALGRLDEITRSATVLTVFERVTE
                     ADRRFTDGVVARCRRDGLRIVTAGYGRLSADGVRDACRNAEAGLELRARLELDLRPPS
                     LSSEFPRRQAMLDRLKRDYPVLDRLAVARRARSRPAPPAVTIVALRVESPDRPENALA
                     PVVERLAVGNRTHVRGRLRLVEHGLWEVRLALSVREIDPADADAAVGLLLLGHVDQTI
                     VDDPLSGMTEGGTVVLASALEPAALGASLPDAWRTAIRAGRIRLFRVAPGDHPIERLL
                     EAAGSLLGAGAARVGESVAIEPIGAVPEADRPPLPLAVRRFGTPGESFDNVARFWSEW
                     IEPRSSEPDNDAVPDPYLAAGAVPACSSSLFDATAGRETVPTVDPAICTGCGRCWESC
                     PDSAIAPVALTARALLESVDESVVHPDDERELAARLARARRQLAARVESSLADGRRRS
                     VGARDWHDAFEWLFAQLRLERAELARARRIGDRWVAELGAIPLSVCDPFFSGARARGG
                     EAELLMLAVNPRTCQGCGICVSVCPESAVGRPARTAEILAGLARTWRRWEALPDTGGA
                     TIARLAESNDMSPVAAVLLSRHALLAVTGSGAEPGSGERLATRQIAAVIEFQRQRRVL
                     RRVRELDELARGLRSAIRETLGAPIEFDDLRTLERALEEPTAAGGVLARLERSADGRL
                     VDERRLRGLLERSRDVEAAARHLREGEHGLGQSRYGVVLHGEAGGAWAGRFPLNPFSV
                     PVALDLARDGAAVAAGLAASLVERAVDDARRVRRAALWLEAPVDLAERERPLAAVDAA
                     ALTEAERAAIAPIVLLADGAALESRGADSLFALLSGDLPIKLVLFDPCDPLASRLDPL
                     LVGLAAREAFVLSASVSAPRQLFDGVSAAIDFPGPALISIHAPSPRRHGFATAATIER
                     ARGAVEAHVHPLLRYDPRRPGVLATRIDLDGNSAVERDRAPDRATASCWARGERRFRD
                     DELLLAARDPAQPTVPGPNGRDPAPGESLALLERIVGRRWETLRELAGGATASTDEIE
                     RSGARPVAEHNAELAALRDEYEARIERQSAMQAERLRKRLRQLVGHGER"
     gene            5340..6671
                     /gene="rsxC"
                     /locus_tag="VD788_10750"
     CDS             5340..6671
                     /gene="rsxC"
                     /locus_tag="VD788_10750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HXV76786.1"
                     /translation="MSASLLGRAATFRHGVHPDELKERTRRLAIERMPFVRRYVVPLS
                     QHAGAPSRALVGQGMRVRRGQLIAAPDGWISTAQHAPVSGSVVAIESWPHPLGTRVPA
                     IVIEIDPFDNQRVPPGDPPRFETMSQGEIVERIQLAGLVGLGGAAFPSHVKLRPPDGK
                     RAAHVVLNGCECEPYLTCDHRVMLERAGDVIEGLRILMRVVGASTGYVGVELNKNDAI
                     DALREVVSGDRSIKLLPLEVKYPQGAEKMLIDAIFHREVPSGGLPLDLDIVVNNVGTA
                     AAIAELFRSGRPLIERVITVSGPGVDRPSNLLVPIGTPIRDVLRHCGMRSDTRQVILG
                     GPMMGMAQKSLEVPVIKGISAVIALTGRAPVVDEEPCIRCGRCLDACPVFLNPSRIAA
                     VVRSDDHGALEGLFVQDCCECASCSFVCPSNIPLVQLMRMGKALVRRGRGG"
     gene            6673..7665
                     /locus_tag="VD788_10755"
     CDS             6673..7665
                     /locus_tag="VD788_10755"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HXV76787.1"
                     /translation="MSETQIRLGITTAPFLKSGLGTRRLMWEVFAALLPVLAAATWYF
                     GVTALLVVSSAVCGALLTEWCLTPSDRRSTLSDGSALLTGVLLGLTLPPSIPLWMAFF
                     GAVVAIALGKSIWGGLGRNLFNPALVGRAFLQAAFPLTLTTWTAPGSGVLAVGDSTWS
                     LPLTRLTDGTTCATPLGLAKFEGRPTATTELFWGQVGGSLGETASAVLIACGIFLALR
                     RVFDWRLPVSTLLTVALLSETLHRVEPSRFPGAAFMLLSGGLLFAAVFMVTDPVTTPL
                     TRRGTWWFGVGVGVLVVLIRLFGGLPEGVMYAVLLMNAVTPLINRYTQPRRFGG"
     gene            7670..8317
                     /locus_tag="VD788_10760"
     CDS             7670..8317
                     /locus_tag="VD788_10760"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HXV76788.1"
                     /translation="MEAHGPQGTLRMVSTLAVAGLLSGLVLVAVQLETEPRILRNRAE
                     ALNQAIYRVLPGTVEIRRWAVRGGALARVDGPSGDGEIYAGHRTDGTLIGYAIPASGP
                     GFMDTIGLIYGYDPGAHRIVGIQVLDSRETPGLGDKIITDETFLRNFDALEIEPSIVP
                     VKHGSKSRSNEVDCITGATISSEAIVAILNASAENWAALLEAGHAPTEVSRNAGR"
     gene            8304..8921
                     /locus_tag="VD788_10765"
     CDS             8304..8921
                     /locus_tag="VD788_10765"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HXV76789.1"
                     /translation="MPAAERPWDELARGLWRENPVFVAVLGLCPALAVTNSLTNGLVM
                     GAATTFVLVGSSLMVSALRKIIPRSVRVPVYVIIIATFVTAVDLLLAAWMPVAHKQLG
                     AFISLIVVNCLILGRQEAFASKHRPRLAVADAVGMALGFTAALALLGAVREILGSGSL
                     LGLRLFGERFEPWTIMVLPPGGFFTLGLLLTVFARVRQRRGQETR"
     gene            8918..9496
                     /locus_tag="VD788_10770"
     CDS             8918..9496
                     /locus_tag="VD788_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HXV76790.1"
                     /translation="MSDLLFIFVSASVINNFTLAYFLGLCPFFGVTGRLDTALRLGLA
                     NIFVMMITAVASWFLNLFVLPHAPYLRLITFIVVIASSVQFVEMVIKKSSPALFRALG
                     IFLPLITTNCAILGLALFATNRGYGLVEGLVYALGAGVGLTLALVIMAGLREEAELSG
                     VPRLVRGAAMGFFVAGILSMAFMGFAGLFHGV"
     gene            9912..11156
                     /locus_tag="VD788_10775"
     CDS             9912..11156
                     /locus_tag="VD788_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV76791.1"
                     /translation="MANRHTVIVGGGIVGLSSAYFLARRGERVTVVERDTPGRGASSG
                     NAGILALGHPPLPHPNAFRQIRRLLFRKTNPLYIAPRADPELLRWLWSFRRACARERF
                     EHSMELLARMGWAAGECIRRLVADEGIDCEYSPSGWLEVFRTRQAMDRARRTADLLRR
                     HGYTVTALDGDALRQREPAFRDEVVGAMHYEDSAFAHPAKLMVGLADRVRKLGVDFLT
                     EAEVVRITLRSSRFTGVELADGQRIDADRAVLAAGAWTTPLARSIGVRVPMQAGKGYH
                     LELRATAHRPSTTCVLAETFVAVTPLDGGLRLAGTVELAGLDLTLNRRRLAMLPRGAA
                     DYLRGIDRAELGGSWCGLRPMTADGLPVLGWAPGVEGVFVATGHAMMGFLLGPLSGKL
                     VAEAIVDGRMSLDAPELAPARF"
     gene            11219..11467
                     /locus_tag="VD788_10780"
     CDS             11219..11467
                     /locus_tag="VD788_10780"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HXV76792.1"
                     /translation="MTEPIKRLYRSRTERQIAGVCGGIGTYSAMDPVVIRLLLVALTA
                     VTGFVPGIVAYVVAWIVTPDEPLPVASVERHAVDTGPG"
     gene            11553..12818
                     /locus_tag="VD788_10785"
     CDS             11553..12818
                     /locus_tag="VD788_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76793.1"
                     /translation="MATVREMSQRDRSVHRFAFVIHPLHVGQIKSSPTLGWTRRLPDG
                     IVERVAAYLPPFPVSLVTGARSPATGQRVEGQLLALGSTPKQMLRKAPEFTYRRLVRA
                     AGLAASGGARLMGLGAFTSVVGDAGVTVACRAPIPITSGNSLTVSATIEAAKQAVRRM
                     GLTELAACRATVVGATGSIGSVCARMLARDVRSIDLVSIEPDKLTALARLIRRETPRV
                     ELTTATTTRGVLGASDLVVSATSAIGQRVLDIAECKPGAVICDVARPPDVSAEEARLR
                     PDVLVIESGEVLIPGEVDFHYDIGLPPGVSYACLAETALLAMDGRFECYTLGRAIELG
                     RVDEIGRLAERHGFRLACLRSVDGVLSDRVIEAKRAQALALRGDEDSLARLRSRAASA
                     LASIQVGAKGVPAVVARGRVSRPSVHAVR"
     gene            12823..13437
                     /locus_tag="VD788_10790"
     CDS             12823..13437
                     /locus_tag="VD788_10790"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HXV76794.1"
                     /translation="MRGFRLALVALGSVVAVCFGPACGPEPAETPRFVVLDEPPPQET
                     VEVARTVTDALGKELVAALFRELESGSAVGAIDVCAVRAQQMTAAYAEQGTRVRRVSR
                     RFRNPANEPDAYESRKLAEFESMQQRGELPTESVAVVLQDGKRTLRYMRPILLKPPCK
                     MCHGPVSEIDRQVLDAIHARYPGDRALGFSVDEVRGAFSVEIDL"
     gene            complement(13445..15403)
                     /locus_tag="VD788_10795"
     CDS             complement(13445..15403)
                     /locus_tag="VD788_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXV76795.1"
                     /translation="MSTDKQRTNDRPDVQPDHLIDTSGMSQGKADALELAEASRESHW
                     AYPTFAGALFMGQLSWSLIHPFPVEESDHDEQGEAFLERLRTFLREQVDADEIDRTGE
                     IPDDVIRGLAEMGAFGIKTPKEYGGLGLSQQYYSRAAMMLGSHCANTAALLSAHQSIG
                     VPQPLLQFGTEEQKRRFLTRTARGEISAFALTEEGVGSDPARMATTADPTEDGRYFVI
                     NGRKLWCTNGTKAGLLVVMAKTPPKIKNGREINQITAFVVEATSPGVTVDYRCRFMGL
                     RALYNAVIDFKDVKVPRENIIAGEGKGLRVALTTLNTGRLTLPANCVGAMRACLSMAR
                     DWSNKREQWGSTIGKHAAIADKIARMASTLFAVEAMTMLTSALVDRKQTDIRVEAAMA
                     KMFGTEEAWNIVYDTMQTIGGRGYETRDSLAGRGETPYPIERVMRDMRINTIFEGSSE
                     IMRLFLAREAMDPHLKAAGEAVNTRLPIGRRFKAALRAAGFYALWYPKQWLPLGGPST
                     AGLEPKLARQVRYVARTSRRLGRRMFHSMLGHGPKMEREQILLGRFVGVGTELFAIAA
                     SCARAQQLIERGTDRDDVLALVDHFCRESRRRIDGYFRGIRDNDDAVGYRLAQRILDS
                     ETDWLYRGMVDADLDTRRRGEPAELMTN"
     gene            complement(15421..16044)
                     /locus_tag="VD788_10800"
     CDS             complement(15421..16044)
                     /locus_tag="VD788_10800"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXV76796.1"
                     /translation="MRSNSESNGQAKPSFIEAARRKQIVDTAIRTIATRGYSQASLAE
                     IAREAGISKGVISYHFEGKVELVAEILSLLLREPAEFIKRRVDAGPRAMDKLRAYVTA
                     NFEFMKTHRDHYVALVDLWESRGSETANRFSAEAYEPSRSYLSRILEKGEASGEFRRL
                     SHTSVASVIQAAIDGVMLQWVFDESAVDLDACRDQILEMVTLYVVIR"
BASE COUNT         2214 a         5565 c         5788 g         2717 t
ORIGIN      
        1 ggctggtcgc cctgctgatc gatctcgaac cggtcccggc ggcaccccac gccgcggtct
       61 cgagcggtat ggcggcggag ccggcagccg caccgctgac cgccgcgccc gaacccgagc
      121 cgaccgcccc cgtcgccgag ccgatctcgt tcgacgatcc gtacgtcgct tccgcgttgt
      181 gcaccacctg caacgaatgc accaatctca atccccggat gttcgtctac aacgcgaacc
      241 gtcaggccac gatcgccgat ccgtccgccg gcaccttcga agaactggtc aaggccgccg
      301 agaagtgtcc ggcgcgttgc atccacccgg gaacgccgcg tgacggcgac ccgacggtcg
      361 acgagcgcct gctggcgcgc gccaaggcgt tcgcgtgaga gcggagcggc gatgagcgag
      421 gtgctggcag ccgcgtcgat catgctcggc ctcgccggtt tcttcggcgt cgttctgtcg
      481 gccgccaatc gctatctccg cgtcgaggag gatccccgga tcgaccaggt ggaagagatg
      541 ctgcccggga ccaactgcgg cgcgtgcggt cagcccggtt gccgcgggtt cgccgaggcg
      601 ctgatcgagg gtcggaccgt gcccggcaag tgcagcgtga gcccggctgc cgaagtggct
      661 cggatcgctt cgtttctcgg cgtcgaagcc ggcgtcgaag agcgccgggt ggcgcgtctg
      721 cattgcgcgg gcggccgatc ctcggtgcgg cgtttggccg actacgcggg gatcgacagc
      781 tgtcgggccg cggtgctggt caacggcggc ggcaaggcct gcccgtgggg ctgtctcggc
      841 ttgggggatt gcgaacgggt ctgcggcttc gacgcgatcc ggatgacgac cgaggacctg
      901 ccgcgcgtga gcgtcctggc gtgcaccgcc tgcggcgact gcgtcgaggt ctgcccgctc
      961 gacctgttca cgctcgagcg cgtctcgcac cgcgtgatcg tccagtgccg cagcccactc
     1021 ggcggcgaac tggcgcgcag cctgtgtcgc gtcgcctgcg acgcatgcgg ccgctgcgcg
     1081 ctcgacgcgc agcagggtgt gatcgagatg caggaaggcc tgccccggct gctcgacccg
     1141 gcacgggccg acgagcgctg tacgctgcac tgtccgaccg gtgcgatccg ctgggtcgtc
     1201 ggcgaccagt tcgcggagaa cgaccgtgtc tgaggcggaa cggcggggct ggcggtggct
     1261 cgacgctctc gccggccggc ggcgggaacg ccggccgagc tttccgctcg atctctggga
     1321 ggcatcgttg tcgagcggtc tcgaggccgt cctcgccgcg gagcgcgggg ccggcagcgt
     1381 ggtgcgccaa gcgatcgacg gcgacggggc cgccgcggtg tcggagagct acggcgcggc
     1441 gatcagcggg cgacgtgcga ccgccgtcgt ctccgacgac gatctcggga gcgtcatcga
     1501 tcggttgcgc accgcggccg cacggcacgc gccgctcgtg atctacgtcg tcgctcgcga
     1561 aaccggcacc gccgagcggc tctcctcgag cggtcgaacg ctcgcgatcg cgcaggaccc
     1621 gcagcacgcg atcgatctcg ggctgatcgc ccgcttcgtc gcggagcgcg cgctcgcgcc
     1681 ggtcgccgtc gtgatcgagg cggagggcgt ggccgacgcg gtcggccgct ggtgtcggcc
     1741 gcccgccgcg ctcgtcgagc actacctcgg tcgggccgac gaccggatcc ccacaccgac
     1801 gccggcccag gcgacgttgt tcggcgagcg tcgccgccgg gtgccgcgct ggttcgaccc
     1861 ggcgcgaccg acggcccacg gcatgcggcc acgaggcccg gcgcgaacgg cggcggagat
     1921 cgggcgggag gtcttcttcg cttcgagcgt tccagtcatt ctcgaccagg cgatcgagac
     1981 gctggccggt ctgaccggac gcgacgccgg tcgggtccgc cggttcggtc cggccgacgc
     2041 acgccacgtc ctgctgctcc acgggtccgc cgtgccgatc gccgcggcga ccgtcctgcg
     2101 cggcgaggcc gccatccggc gcaccgccgt cgtcgcgatc gactggtgga cgaccgaggc
     2161 gctcggacgc ctcgacgaga tcacgcggtc ggccacggtg ctgaccgtct tcgagcgagt
     2221 gaccgaggcc gatcggcgct ttacggacgg tgtggtcgcg cgctgccggc gtgacgggct
     2281 ccgcatcgtg accgccggct acgggcggct ctcggccgac ggagtgcgcg acgcttgccg
     2341 caacgccgag gcgggtctcg agctccgcgc gaggctcgag ctcgatctgc gtccgccttc
     2401 gctttcgtcc gagttcccgc ggcgccaggc gatgctcgat cgactgaaac gcgactaccc
     2461 ggtactcgac cggctcgccg ttgcacgacg ggcgcggagc cgaccggccc cccctgcggt
     2521 cacgatcgtc gcgctccgcg tcgaatcgcc cgatcgcccg gaaaacgcac tcgcgccggt
     2581 cgtcgaacgg ctcgccgtcg gcaaccggac ccacgtgcgc ggccggctgc gtctcgtcga
     2641 acacggtctg tgggaagtgc ggctggcgtt gtcggtgcgc gagatcgacc ccgcggacgc
     2701 ggacgcggcc gtcgggttgc tgctgttggg acacgtcgac cagacgattg tcgacgatcc
     2761 gctctccggg atgaccgagg gcggcaccgt cgtcctcgcc tcggcgctcg agccagcggc
     2821 gctcggggcc tcgctgcccg acgcctggcg aacggcgatc cgagcggggc gaatcagact
     2881 gttccgcgtc gcgccgggcg atcacccgat cgagcggctg ctcgaggcgg ccgggtcgct
     2941 gctcggcgcc ggtgcggcgc gcgtcggtga gtcggttgcg atcgaaccga tcggtgcagt
     3001 cccggaggcc gaccgaccgc cgctgccgct cgcggtgcgc cgcttcggca cgccgggcga
     3061 gagcttcgac aacgtggcgc gcttctggag cgagtggatc gagccgcggt cgagcgagcc
     3121 cgacaacgac gcagtgcccg atccctatct ggcggccggc gcggttccgg cctgcagctc
     3181 gagtctgttc gatgcgaccg ccgggcggga gaccgttccg acggtggatc cggcgatctg
     3241 caccggctgc gggcggtgct gggagtcgtg ccccgactcg gcgatcgcgc cggtcgcgct
     3301 gaccgcacgc gcgctgctcg agtcggtcga cgaatcggtc gtccaccccg acgacgagcg
     3361 cgagctggcc gcgcgactcg cccgcgcgcg gcgtcagctc gccgcgcggg tcgaatcgag
     3421 cctggccgac ggccgtcggc ggtcggtcgg cgcgcgagac tggcacgacg ccttcgagtg
     3481 gttgttcgcg cagcttcggc tggagcgggc cgaactggca cgcgcgcgac ggatcggcga
     3541 ccgatgggtc gcggagctcg gcgcgatccc gctctccgtc tgcgacccgt tcttctccgg
     3601 cgcccgcgcg cgcggcggcg aggcggagtt gctgatgctc gcggtcaacc cgcgaacttg
     3661 ccagggttgc ggcatctgcg tctccgtctg tcccgagagc gccgtgggtc gaccagcgcg
     3721 aacggccgag atcctcgccg gcctggcgcg gacgtggagg cggtgggagg ccctgcccga
     3781 caccggcggg gcgacgatcg cccgtctcgc cgaatcgaac gacatgtcgc ccgtggccgc
     3841 ggtcctgctc tcgaggcacg cgctgctcgc ggtgacgggg agcggcgccg agccgggatc
     3901 cggcgaacgg ctggccacgc gtcagatcgc cgcggtcatc gagttccagc ggcagcgacg
     3961 tgtgctcagg cgcgtccgcg agctcgacga gctcgcgcgc ggcttgcggt ccgccatccg
     4021 cgagacgctc ggcgcgccga tcgagttcga cgacctgcgg actctcgaac gggcgctcga
     4081 agaaccgacc gccgccggcg gagtcctggc acggctcgag cggtccgccg acgggcgcct
     4141 cgtcgacgag cggcgattgc gcggtctgct cgaacggagc cgcgacgtgg aggcagccgc
     4201 gcgacatcta cgcgagggcg agcacggcct cgggcagtcg cgctacggcg tcgtgctcca
     4261 tggcgaggcc ggtggtgcgt gggccggccg ctttccgctg aacccgttca gcgtgccggt
     4321 cgcgctcgac ctcgcgcggg acggcgccgc ggtcgcagcc ggtctggccg cgtcgctcgt
     4381 cgaacgggcg gtggacgacg cgcgccgcgt gcgccgcgcg gcgctgtggt tggaggcccc
     4441 cgtcgacctc gcggaacggg agcggccgct cgccgcagtg gacgccgcgg cactgaccga
     4501 ggcggagcgg gccgcgatcg cgccgatcgt gctgctggcc gacggcgccg cgctcgaatc
     4561 gcgcggcgcc gactcgctgt tcgcgctgct gtcgggcgac ctgccgatca agctcgtgct
     4621 gttcgatccg tgcgatccgc tcgcgtcgcg gctcgacccg ttgctcgtcg ggctcgccgc
     4681 ccgcgaagcc ttcgtgcttt ccgcctcggt ctccgcgccg cgccagctgt tcgacggcgt
     4741 atcggccgcg atcgactttc ccggccccgc gctgatctcg atccacgcac cgagtccgcg
     4801 gcggcacggc ttcgccaccg cggcgacgat cgagcgcgct cgcggggcgg tcgaggccca
     4861 cgtgcacccg ttgctgcgtt acgacccccg gcgccccggc gtgctcgcca cgcggatcga
     4921 cctcgacggc aactcggccg tcgagcgaga ccgcgcgccg gaccgggcga ccgcgagctg
     4981 ctgggcccgc ggcgagcgca gattccgaga cgacgaactt ctcctcgcgg cgcgggatcc
     5041 cgcccaaccc accgttcccg gcccgaacgg aagggatccc gcgcccgggg agagcttggc
     5101 gctgctcgag cggattgtcg gccgtcgctg ggagacgctg cgcgagctgg ccggcggcgc
     5161 cacggcgtcc accgacgaga tcgagcgatc cggtgcacga ccggtcgcgg agcacaacgc
     5221 cgagctcgcc gcgctacgcg acgagtacga ggcgcggatc gaacgccaga gcgcgatgca
     5281 ggccgagcgt ctccgcaagc ggctgcggca gctcgtcggt catggcgaga ggtagcatcg
     5341 tgagtgcgtc gttgctcggt cgcgcggcca cctttcgtca cggcgtgcac cccgacgagc
     5401 tgaaggaacg aacgcggcgc ctcgcgatcg agcggatgcc gttcgtccgg cgctacgtcg
     5461 tgccgctcag ccagcacgcc ggcgccccgt cgcgggcgtt ggtcggtcag ggaatgcgcg
     5521 tccgccgggg ccagctgatc gccgcccccg acggttggat ctcgaccgcg cagcacgcgc
     5581 cggtcagcgg ctccgtggtg gcgatcgagt cctggccgca tccgctcggc actcgggtgc
     5641 cggcgattgt catcgagatc gatccgttcg acaaccagcg cgtcccgccc ggcgatccac
     5701 ctcggttcga gacgatgtcc caaggcgaga tcgtcgagcg gatccagctc gcgggcctcg
     5761 tcggtctcgg cggcgcggcg tttccgagcc acgtcaagct gcgtccgccc gacggcaagc
     5821 gggcagccca cgtcgtgctc aacgggtgcg agtgcgagcc gtacctgacc tgcgatcacc
     5881 gcgtgatgct cgaacgcgcg ggcgacgtga tcgaaggcct gcgcatcttg atgcgagtcg
     5941 tcggcgcgtc gaccgggtac gtcggcgtcg agctgaacaa gaacgacgcg atcgacgcgc
     6001 tgcgcgaggt cgtgagcggc gaccggtcga tcaagctgct tccgctcgag gtcaagtacc
     6061 cgcagggcgc cgagaagatg ctgatcgacg cgatcttcca tcgtgaggtg ccgagcggcg
     6121 ggttgcccct cgacctcgac atcgtcgtca acaacgtcgg caccgcggcc gcgatcgctg
     6181 agctgtttcg cagcgggagg ccgctgatcg agcgggtcat caccgtgagc gggccgggtg
     6241 tcgatcgacc atcgaacctg ctggtgccga tcggcacgcc gatccgcgac gtgctgcggc
     6301 actgcgggat gcgctccgac acgcgccagg tgatcctcgg cgggccgatg atgggcatgg
     6361 cacagaaatc gctcgaagtg ccggtgatca agggcatctc ggcggtgatc gcgctcaccg
     6421 gccgtgcgcc ggtggtcgac gaggaaccgt gcatccgttg cggccgttgc ctcgacgcct
     6481 gtccggtctt tctcaatccg tcgcgcatcg cggccgtcgt ccgttccgac gatcacggcg
     6541 cactcgaagg cttgttcgtg caggactgtt gcgagtgcgc cagctgttcg ttcgtctgtc
     6601 cgtccaacat tccgctggtc cagctgatgc gcatgggtaa ggcgctcgtg cgacgcgggc
     6661 gagggggctg acgtgtcgga gacgcagatc cggctcggca ttaccaccgc gccgtttctc
     6721 aagagcgggc tcggtacgcg ccggctgatg tgggaagtgt tcgcggccct gctgccggtg
     6781 ttggccgccg ccacgtggta cttcggcgtg accgcgctgc tcgtcgtgtc gagcgccgtc
     6841 tgcggcgcgt tgctgaccga gtggtgcttg accccgtccg atcgccggtc cacgctgtcg
     6901 gacggcagcg cgctgctgac cggcgtgctg ctggggctga ccctgccccc gtcgatcccg
     6961 ctatggatgg ccttcttcgg cgcggtcgtg gcgatcgcgc tgggcaagtc gatctggggc
     7021 gggctgggac ggaacctgtt caatcccgcg ctcgtcggcc gagcgttctt gcaggccgcg
     7081 tttccgctga cgctgacgac atggaccgcg cccggctccg gcgtgctcgc cgtgggggac
     7141 agcacctggt ccttgccgct gacacggctg accgacggga cgacctgtgc gacgccgctc
     7201 ggtctggcca agttcgaagg tcgaccgacg gcgacgaccg agctgttctg gggacaggtc
     7261 ggcggctcgc tcggcgagac cgcgtcggcg gttttgatcg catgcgggat cttcctcgcg
     7321 ctccgccgcg tgttcgattg gcgtctgccc gtctcgacgt tgctgacggt cgccctgctg
     7381 agcgagacgc tccaccgggt cgagccgagc cgattccccg gcgccgcgtt catgctgctg
     7441 tccggcggct tgttgttcgc cgcggtgttc atggtcaccg acccggtgac gacgccgttg
     7501 acccgccgcg gtacctggtg gttcggcgtc ggcgtcggcg tgctcgtcgt gttgatccgg
     7561 ctgttcggtg gtttgcccga gggcgtcatg tatgcggtgc ttttgatgaa cgcggttacg
     7621 ccgttgatca accgctacac gcagccgcgg cggttcggag gctgacctga tggaagccca
     7681 cgggccgcaa ggcacgctgc ggatggtgtc gacgctggcc gtcgccgggc tgctgtcggg
     7741 cctggtgctc gtcgccgtcc agctcgagac cgaaccccgc atcctgcgca accgcgccga
     7801 ggcgttgaat caggcgatct accgcgtgct gccgggaacg gtcgagatcc gacgctgggc
     7861 cgtgcgcggt ggtgcgctgg cgcgcgtcga cggtccgagc ggcgacgggg agatctacgc
     7921 gggccaccgc acggacggca cgttgatcgg ttacgcgatc ccggcttcgg gtcccggctt
     7981 catggacacg atcgggttga tctacggtta cgacccgggg gcgcaccgga tcgtcgggat
     8041 ccaggtgctc gacagccgcg agactccggg tctcggtgac aagatcatca ccgacgagac
     8101 gttcctccgg aatttcgacg cgctcgagat cgagccgtcg atcgtgccgg tcaagcacgg
     8161 gtcgaagtcg cggtcgaacg aggtcgactg catcaccggt gcgacgatct cctcggaggc
     8221 gatcgtcgcg atcttgaacg cgagcgccga gaactgggcg gcgctgctcg aggcgggtca
     8281 cgcgccgacc gaggtcagtc gcaatgccgg ccgctgagcg tccgtgggac gagctcgccc
     8341 gagggctgtg gcgcgagaat cccgtcttcg tcgcggtgct cggcctgtgt cccgcgctgg
     8401 ccgtgacgaa ctcgctgacc aacgggctcg tgatgggtgc cgcgacgacg ttcgtgctcg
     8461 tcggctcgag cctgatggtg tctgcgctgc gcaagatcat cccgcgctcg gtgcgcgtgc
     8521 cggtctacgt gatcatcatc gcgaccttcg tcaccgccgt cgatttgctg ctggccgcgt
     8581 ggatgcctgt ggcgcacaag cagctcggcg ccttcatctc gttgatcgtg gtcaactgcc
     8641 tgatcctcgg ccgtcaggag gcgtttgcct cgaagcatcg tccgcgcctg gccgtcgccg
     8701 atgcggtcgg gatggcgctg gggttcaccg cggcgctggc gctgctcggt gcggtccgcg
     8761 agatcctcgg cagcgggtcg ctgctcggtc tgcgcctgtt cggcgagcga ttcgagccgt
     8821 ggacgatcat ggttctgcct cccggcggct tcttcacgct cggtctgttg ttgaccgtct
     8881 ttgcccgggt gcgccaacgg cgcggccagg agacgcgatg agcgacctgc tgttcatctt
     8941 cgtgtcggcc agcgtgatca acaatttcac gctggcctat ttcctcgggc tctgtccgtt
     9001 cttcggcgtg accggtcgcc tcgacaccgc gctgcggctg ggcctggcca acatcttcgt
     9061 gatgatgatc accgccgtcg cgtcgtggtt tctcaacctg ttcgtgctgc cgcacgcgcc
     9121 ctacctgcgg ctgatcacct tcatcgtcgt cattgccagc tcggtgcagt tcgtcgagat
     9181 ggtgatcaag aaatcgagtc ccgcgctgtt tcgagcgctc ggcatcttcc tgccgttgat
     9241 cacgaccaac tgtgcgattc tcggtctggc actgttcgcg accaaccgcg ggtacggatt
     9301 ggtcgagggt ctggtctatg cgctgggggc cggggtggga ctgaccctgg cgctcgtgat
     9361 catggcgggc ctgcgtgagg aagcggagct gtccggcgtt ccgcgtctgg tacgcggcgc
     9421 ggcgatgggc ttcttcgtcg ccggcattct gtcgatggca ttcatgggat tcgccggtct
     9481 gttccatggt gtctgacctc gccggcgtcg tggatccgca gcaaggcctg accgcgcttg
     9541 gtacagactg ccgatttggc gacgctgcac agcggctctt ggcactcgta ctcgcacacc
     9601 gagacgtctt cgggtacagt ttggacgacc tgctcgacgc accagtcgcg cacgctgcgc
     9661 acgagcgtac cgaccgcgat cggtcgaact acgatcgtaa actgggttcc catcggcctc
     9721 cgtccggtcg gcgatcgcag cgttgtttac cggtagttca gcgtcacggc aatgcgaaat
     9781 gcgcgttgcg cgtgccacgg agacgcggca ctgcgcccgg cgccccggtg gtcggtcgag
     9841 acgaaggcgg gccgttgacc gcgaccggcg cgtgagccaa ctttgaccgg gtcctcggcg
     9901 gaaacgagcg aatggcgaac cggcacacag tgatcgtcgg cggggggatc gtcgggctct
     9961 cgtcggccta ctttctcgcg cgtcgcggtg agcgcgtgac cgtggtcgaa cgagacacgc
    10021 ccggccgcgg tgcatcttcc ggtaacgccg gaatcctcgc tctcggccac ccgccgctgc
    10081 cgcacccgaa cgcgtttcgc cagattcggc gcttgctgtt tcgcaaaacc aatccgttgt
    10141 acatcgctcc gcgcgccgat ccggagctgc tgcgctggct ctggtcgttc cgccgggcct
    10201 gcgcacgcga acgcttcgag cattcgatgg agctgctggc ccggatgggc tgggccgcgg
    10261 gcgagtgcat ccggcgactg gtggcggacg aggggatcga ctgcgagtac agcccgagcg
    10321 gctggctcga ggtcttccgc acccggcagg cgatggatcg tgcgcggagg acggcggatc
    10381 tgctgcgccg tcacggctac accgtgaccg ctctcgacgg cgacgcgctg cgccagcgcg
    10441 agccggcgtt ccgggacgag gtcgtcggcg cgatgcacta cgaggacagc gcgttcgcgc
    10501 accccgccaa gctgatggtc ggtctggcgg accgcgtccg gaaactgggt gtcgatttct
    10561 tgaccgaagc cgaggtggtc cggatcacgc tgcgttcctc gcgcttcacc ggagtcgaat
    10621 tggccgacgg ccagcggatc gacgccgatc gggccgtgct cgccgcggga gcgtggacca
    10681 cgccgctcgc gcgctcgatc ggcgttcggg tgccgatgca ggcgggcaag ggctaccacc
    10741 tcgagctccg cgccaccgcc caccgaccga gcacgacgtg cgtcctggcc gagaccttcg
    10801 tcgcggtcac gccgctcgac ggcgggttgc gcctcgccgg cacggtcgag ctcgcgggac
    10861 tcgatctcac gctcaaccga cggcggctcg cgatgctccc tcgcggggcg gccgactacc
    10921 tgcgcggaat cgaccgggcc gagctcggcg gcagctggtg cggcctgcgg ccgatgaccg
    10981 ccgacgggct cccggtgctc ggctgggcac ccggcgtcga gggggttttc gtcgccaccg
    11041 gccacgcgat gatggggttc ctgctcgggc cgctcagcgg aaagctggtc gccgaggcga
    11101 tcgtcgacgg gcgcatgtcg ctcgacgccc ccgagctcgc ccccgcccgc ttctgagccg
    11161 gcgcggtgga accgccgtca cacacgctgc gtatcaacgg gcgggagggc ccgtcgagat
    11221 gaccgaaccg atcaagcgcc tctatcgatc gcggaccgag cggcagatcg ccggggtttg
    11281 cggcggaatc ggcacgtaca gcgcgatgga cccggtcgtg attcgactgc tgctggtagc
    11341 gctgacggcg gtgaccgggt tcgttccggg aatcgtcgcc tacgtcgtcg cctggatcgt
    11401 cacacccgac gagccgctgc cggtcgcctc ggtcgagcgg cacgcggtcg acaccggacc
    11461 cggctaattc ggaccttttc ggtgccgagc cgactcccgc gacgcaccgg cgatccgagg
    11521 aacccgtgta atctgtaagc gagaggcgag ccgtggccac ggtgcgggag atgtcccaac
    11581 gagacaggtc ggtccaccgc ttcgctttcg tcatccatcc actacacgtc ggccagatca
    11641 agagcagtcc gaccctcggt tggacccgac ggctgcccga cggcatcgtc gagcgggttg
    11701 cggcctacct gccgccgttt cccgtctcgc tcgtgaccgg ggcgcgctcg ccggccaccg
    11761 gccagcgggt cgaggggcag ttgctcgccc tcgggagcac gccgaagcag atgctgcgga
    11821 aggcgcccga gttcacctat cgccgtctgg tccgcgcggc cggtctcgcc gcgagcgggg
    11881 gagcccgact gatgggtctc ggcgcgttca cgagcgtggt cggcgatgcc ggggttaccg
    11941 tcgcgtgccg cgctccgatc ccgatcacca gcggcaacag cctcaccgtc tccgccacga
    12001 tcgaggccgc caagcaggcg gtccgacgga tgggattgac cgaactcgcc gcttgtcgcg
    12061 cgacggtcgt cggcgcgacc ggctcgatcg gctcggtctg cgcgcgcatg ctggcacgcg
    12121 acgtccggtc gatcgatctg gtctcgatcg agcccgacaa gctgaccgcg ctcgcccgcc
    12181 tgattcgtcg cgagacgccc cgcgtcgagc tgaccaccgc gacgacgact cgcggcgtgc
    12241 tcggcgcgag cgacctggtc gtcagcgcga cgtcggcgat cggacaacgc gtgctggata
    12301 tcgcggaatg caagccgggc gccgtgatct gcgacgtcgc acgaccgccc gacgtctcgg
    12361 ccgaggaggc ccggctgcgg ccggacgtgc tggtgatcga gagtggcgag gtgctgatac
    12421 cgggtgaggt cgatttccac tacgacatcg gcctgccgcc cggggtatcc tacgcgtgcc
    12481 tcgccgagac cgcgttgctc gcgatggacg gtcgcttcga atgctatacc ctcggacggg
    12541 cgatcgagct cgggcgggtc gacgagatcg gacgcctggc cgagcggcac ggcttccgct
    12601 tggcatgcct gcgttccgtc gacggcgtgc tgtcggaccg cgtgatcgaa gcgaagcgcg
    12661 ctcaggcgct ggcgctccgc ggcgacgagg acagtctggc acgcctgcgc agccgagccg
    12721 cgagtgcgct ggcgtcgatc caggtcgggg caaagggcgt gccggcggtc gtggcccgcg
    12781 ggcgcgtgtc gcgcccgtcg gtgcacgcgg tgaggtgagg tcatgcgagg attccgactg
    12841 gcgctggtcg cgctcgggtc ggtcgtcgcc gtctgcttcg gtccggcgtg cggacccgag
    12901 ccggctgaga cgccgcgctt cgtcgtgctc gacgagccgc caccgcagga gaccgtcgag
    12961 gtggctcgca cggtcaccga cgcgttgggc aaggagctgg tcgccgccct gttccgcgag
    13021 ctcgagagcg gaagcgcggt cggtgcgatc gacgtctgcg cggttcgcgc ccaacagatg
    13081 acggcggcct acgccgagca ggggacgcgc gtgcgtcgcg tcagccggcg gttccgcaat
    13141 cccgccaacg aaccggacgc gtacgagagt cgcaagctgg ccgagttcga gtcgatgcaa
    13201 caacgcggcg agctgccgac ggaatcggtc gcggtcgtgc tgcaggacgg caagcgcacg
    13261 ctgcgctaca tgcgaccgat cctgctcaag ccgccgtgca agatgtgtca cggaccggtg
    13321 agcgagatcg accgacaggt gctggacgcg atccacgcgc ggtacccggg cgaccgcgcc
    13381 ctcggcttct cggtcgacga agtgcgcggc gcgttctccg tcgagatcga tctgtagcgg
    13441 accgtcagtt cgtcatcagc tccgccggct caccgcggcg ccgcgtgtcg agatcggcgt
    13501 cgaccatgcc acgatagagc cagtccgttt cggaatcgag tatgcgctgg gccagccggt
    13561 agccgacggc gtcgtcgttg tcacgaatac cgcggaagta accgtcgatc cgccggcgtg
    13621 attcgcggca gaagtggtcg acgagcgcca gcacgtcgtc ccggtcggta ccccgctcga
    13681 tcagctgttg ggcgcgagcg cagctggcgg cgatcgcgaa caactcggtg ccgacgccga
    13741 cgaagcggcc gagcaatatc tgctcgcgct ccattttcgg tccgtggccc agcatgctgt
    13801 ggaacatgcg gcggccgagc cggcggctgg tccgcgcgac gtagcgcacc tggcgggcga
    13861 gcttcggttc gagaccggcc gtcgacgggc caccgagcgg cagccactgt ttcggatacc
    13921 acagcgcgta gaagccggcg gcccgcagcg ccgccttgaa ccggcggccg atcggcagcc
    13981 gggtgttgac cgcttcgccc gccgctttga gatgcgggtc catcgcctcg cgcgcgagga
    14041 acagtcgcat gatctcgctc gatccctcga agatcgtgtt gatgcgcatg tcgcgcatca
    14101 ctcgctcgat cgggtacggg gtctcaccac gcccggcgag cgagtcacga gtctcgtagc
    14161 cacgaccgcc gatcgtctgc atcgtgtcgt agacgatgtt ccacgcctct tcggtgccga
    14221 acatcttggc catcgccgcc tcgacacgga tatcggtctg cttgcggtcg acgagggccg
    14281 aggtgagcat cgtcatcgcc tcgacggcga acaaggtcga ggccatgcgg gcgatcttgt
    14341 ccgcgatcgc ggcgtgcttg ccgatcgtcg agccccattg ctcgcgtttg ttcgaccagt
    14401 ccctggccat cgacagacac gcgcgcatcg cgccgacgca gttcgccggc agtgtcagac
    14461 gaccggtgtt gagcgtggtc agcgcgacgc gcaagccctt gccctcgccg gcgatgatgt
    14521 tttcgcgcgg gaccttgacg tccttgaagt cgatcaccgc gttgtacaac gctcggaggc
    14581 ccatgaaccg gcagcggtag tcgacggtga ccccggggct cgtggcctcg acgacgaacg
    14641 cggtgatctg gttgatctcg cggccgtttt tgatcttggg tggggtcttg gccatcacga
    14701 ccagcaaccc ggccttggtg ccgttggtgc accacagctt gcggccattg atgacgaagt
    14761 accggccgtc ctccgtcggg tcggcggtgg tcgccatccg cgccgggtcc gatcccacgc
    14821 cttcctcggt cagcgcgaac gcggagatct cgccccgcgc ggtacgggtg aggaatcgcc
    14881 gcttctgctc ctcggtgccg aactgcaaca gcggctgggg cacgccgatc gactggtgcg
    14941 ccgacaggag cgccgcggtg ttggcgcagt ggctgccgag catcatcgcg gcgcggctgt
    15001 agtactgctg ggacagaccg agcccgccgt actccttggg cgtcttgata ccgaacgcgc
    15061 ccatttcggc caagccccga atcacgtcgt ccggtatctc gccggtgcgg tcgatctcgt
    15121 cggcgtccac ctgctcgcgc agaaacgtcc gcagacgctc gaggaacgcc tctccctgct
    15181 cgtcgtggtc cgactcctcg acggggaagg ggtgaatcag gctccacgac agttgaccca
    15241 tgaagagggc accggcgaac gtcgggtagg cccagtgcga ctcgcgcgag gcctcggcca
    15301 gttccaaggc gtcggccttg ccttgcgaca taccgctcgt gtcgatgagg tggtccggtt
    15361 ggacatccgg ccggtcattg gtgcgttgct tgtcggtact catcgggtgt tcctcctgac
    15421 tcaacgaatc accacgtaca acgtgaccat ctcgagtatc tggtcacggc aggcgtccag
    15481 atcgacggcg ctctcgtcga agacccactg cagcatcaca ccgtcgatcg ccgcctggat
    15541 caccgaggcg acgctcgtgt ggctcagcct gcggaactcg ccgcttgcct cgcccttctc
    15601 gaggatgcgg gacaggtagc tgcggctcgg ttcgtacgcc tccgcgctga agcggttggc
    15661 ggtctccgag ccgcgactct cccacagatc gacgagagcg acgtagtggt cgcggtgcgt
    15721 cttcatgaac tcgaaattgg cggtgacgta ggcccgcagc ttgtccatcg cacgcggtcc
    15781 ggcatcgacg cgccgcttga tgaactcggc cggctcgcgc agcagcaggg agaggatctc
    15841 cgcgacgagc tcgaccttgc cctcgaagtg ataggagatc acgcccttgc tgatgccggc
    15901 ctcgcgtgcg atctcggcga gcgacgcctg ggagtacccg cgcgtggcga tcgtgcggat
    15961 cgcggtgtcg acgatctgct tgcgccgtgc cgcctcgatg aaggacggtt tggcttgtcc
    16021 gttcgattcg gaatttgacc gcatggttaa aagataggtc ctgtggccaa tggcggcaac
    16081 gccaaaaacg gaccggcggc tgttatcgcc actcgattta taattttttg cagaagatgg
    16141 tggacgggct cgggctcagg gtcgatcggt cggaccgacc gatcggatga aattgacgag
    16201 gtcccagcgc acgttctcgc tgagccgctg accctctccc gacatcttcc cgtggccgtg
    16261 ggtcaggatg tagaaccact cccc
//
LOCUS       DASPGN010000376         6917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_185052, whole genome shotgun sequence.
ACCESSION   DASPGN010000376 DASPGN010000000
VERSION     DASPGN010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185052"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..3884)
                     /locus_tag="VD788_10805"
     CDS             complement(120..3884)
                     /locus_tag="VD788_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV76797.1"
                     /translation="MRASRIRFLLRLSFAVATSAAVAAPGELRLRFAGGEFRPAAPQT
                     PPAWFSTDRSAPAPRGERCLVAIAERALEPAERHRLEATGARLLGYLPDNGYRLCVSP
                     GAESAIRSLPFVAWLGAAPPHLEIAAPLAAAAADPAGDVRLRAILAPGEMPGRALGIL
                     AGLAPTAAPAGPDLAWRVSATVPAARLRVVLSALAALPEVVAVEPVRRVVPLNQDGVW
                     VHQSFVGPAAQQTPIFDRGIFGCGQVISVADTAQDYDSCQFRDPAGPPPVLGCATPPC
                     PAGTPDLSLRKDILYYNWSSTATGEEDTCPTLFLGGSGHGTHTSGSAAGDNAPWADCT
                     TFVSPNRNAGDGQAPGARLVVIELGDGLEYLNELGGSIWNIADVAFQSGARIHSFSFG
                     GVCYDALGQCVPGCELPYDSLARDADLAMWTYPDLLLVNAAGNSGTLCLPPDSVVTPA
                     IAKNVLAVGGLEHGASANAVMPESSRGPVFDGRLRPTVAAQGRAVVSAASDADLGTLD
                     CSTCSLDGTSMSAPTVAGLAALVREYYAAGFHVSGVRDAGAGISPSGALLKATLIGAA
                     VDIAGAGPDHDSGFGRVQLDTVLPFDGASFRLLVDDHRQGLTTGSLVQHAIDVTAGET
                     LAATLVWTDYPAEPNAATARVNELHLEVVDPTGNVWFQTLDPLDGTPAATDDPTDEAD
                     ELNTEEQLVFASPAAGRWVVRVSGVSVPWGPQPYALVVRGAFDECAAPPAPAVPALAT
                     PADGQVQLSWAPVDGAASYAVERALAACPDGPWVRLATGVAATTYLDVDVSGGATYSY
                     RVIAASDAAGACESPPSSCAEVQPTGDCTLTPVFAGVASAVSQGGATCGVVLDWSDGA
                     AICPGDVVYNVYRDTSPDFEPAPANLIASCLSGTGYTDRSGLAYGTEYQYVVRAEDAA
                     PGHGGPCRDGNQDLNLARAAAAPDGPATIGQWSDDAGDTGAAQFNRGPSWGIDETGGD
                     SGPRAYRASSSQLVCTDLTSPPLTLAEPAAGPELSFSTKHALDYDPGVILFYEGSIGQ
                     VEIASGPAYSNWTRVPLAPDYPAYIDAPFYNVCPTTQAVGQYFSDIDTTWDTYTASLV
                     NWGGQDVRIRFHLSGDLYFSGGDWWIDDVLVTQAAVPGSCATGGAAPPPVPDGATVSG
                     APMRAVRAGDDVVLSWDAAQCPAAAVNVYRGSIGDFTTFTGGDCDLQATGTATLAIPD
                     DSWFLVVATDGADTDGSWSRDHTGAELGYSGAGTVCLSIVAHAADGVCPD"
     gene            4029..5747
                     /locus_tag="VD788_10810"
     CDS             4029..5747
                     /locus_tag="VD788_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76798.1"
                     /translation="MVSNPFIRAVPVVAALAALVSCVGPATEKDRIVLERTGAGDGRW
                     RATYLLGEPAGELRFRRQAGFYREAVWTIASPGWRLDRRGDDQTIVAGPDADRRRLVV
                     EFPQHTDELAKEYEFFAEFSDGSVAVYTGHLYLTDEPPPRPGAEDDADFLVRVELRPR
                     DDEQIVVRGRTARGRTVWDDPEGDGTYVYFGGIEPLETDAMIAVIDPGAPPWLRDELG
                     RLLPEMFARYTTEFGSRLPWKPVVLFSFDGEGSGLSSGGGTLTGLVQMSARGSAWYDA
                     SPDAAEHLLYLIAHEAAHLWNGQFHPYRDDSDSWMHEGSADAFAELALLRSGTIDAAR
                     LAERRTEALGRCIDGLRGVSLRESGTSGRFDNYYGCGNVLALWSVAALGDAGEPDRLF
                     DLWRAVFAHADDDGRYDRQSYFDALATLGVEAERVGELREFLDTVHADPAAAIRALLA
                     RAGLRLVPLDRPVPSRRRARATAAVAHLMRAACDGWYSFYTEWPSIRSATSESCEPFR
                     AELRIVRLEGHGIADDGDRGYDAVAERCRAGLPVRLGLDGGGAVSVPCETVLPALLPP
                     LAVPQL"
     gene            complement(5763..6683)
                     /locus_tag="VD788_10815"
     CDS             complement(5763..6683)
                     /locus_tag="VD788_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76799.1"
                     /translation="MNGRRWLWLGCLGLGAVAAVAAIVGGVVSLTALRQIRSERAERD
                     SVEHRLPPQRSARRAPEADGDRSTAPSGRGRVVLAVSVAQLTVLPAAAGEFVRLETRY
                     DPNRYALDERFERAADGSWTYRIRFEPSGSSMLALLRVKLGGPLPKLRLSLPRGVPLV
                     LNGEVRSGVGAVELGGLIVESAELRAEQGALSLSFAEPLAEPMEWLSLVGDKGSVEIT
                     GLGNASPDRTSVRQHLGEIDLDLRGRWVRDGEIRVGAALAGGTIWLPEGVTILGLEDR
                     LEPVRERSELPRPVLTFSLERRGGSLAVVE"
BASE COUNT         1001 a         2455 c         2548 g          913 t
ORIGIN      
        1 ccgaatcccg gcgggcgact accaggtcga catcctgatc acctacgaga acggcgacgt
       61 cctggcccac gcccgtgccc cgctgcaggt gacgccggcg gcgaactgac cgcgagccgt
      121 caatcggggc agacgccgtc cgcggcgtgc gccacgatcg agagacagac ggtgcccgcg
      181 cccgagtagc cgagctccgc accggtgtgg tcgcggctcc agctgccgtc ggtgtcggcg
      241 ccgtcggtcg ccacgacgag gaaccaactg tcgtcgggga tcgccagcgt ggccgtgccg
      301 gtcgcctgca ggtcgcagtc gccgccggtg aacgtggtga agtcgccgat cgatccgcga
      361 tagacgttga ccgccgccgc ggggcactgc gccgcgtccc agctgagcac gacgtcgtcg
      421 cccgcgcgca ccgcccgcat cggcgcgccg gagaccgtcg caccgtcggg caccgggggt
      481 ggcgcggccc cgccggtcgc gcagcttccg gggacggcgg cctgggtgac gaggacgtcg
      541 tcgatccacc agtcgccgcc cgaaaaatac aggtcgcccg acagatggaa gcggatgcgc
      601 acgtcctggc cgccccagtt gacgagcgac gcggtgtagg tgtcccacgt cgtgtcgatg
      661 tcgctgaagt actggcccac ggcctgcgtc gtcgggcaga cgttgtagaa cggtgcgtcg
      721 atgtaggccg ggtagtcggg cgccagcggc acgcgtgtcc agttcgagta ggccggaccg
      781 ctcgcgatct cgacctgtcc gatcgagccc tcgtagaaca ggatcacgcc cgggtcgtag
      841 tcgagcgcgt gcttggtcga gaacgacagc tcgggacccg cagccggttc cgcgagcgtc
      901 agtggcggcg aggtcagatc ggtgcagacg agctgcgagc tcgaggcgcg ataggcgcgc
      961 ggcccgctgt cgccgccggt ctcgtcgatg ccccaggacg ggccgcggtt gaactgcgcc
     1021 gcacccgtgt cgccggcgtc gtcgctccac tggccgatcg tcgccggacc gtcgggcgcg
     1081 gcggcggcgc gcgccagatt gaggtcctga ttgccgtcgc gacaagggcc gccgtggccg
     1141 ggcgccgcgt cctcggcacg gacgacgtac tggtactcgg taccatacgc gagcccggag
     1201 cggtcggtgt agccggtccc gctcaggcaa ctcgcgatca ggttcgccgg cgcgggctcg
     1261 aaatcgggcg aggtatcgcg atagacgttg tagacgacgt cgccggggca gatcgccgcg
     1321 ccgtcgctcc agtcgagcac gacgccgcag gtcgcgccgc cctggctgac agccgaggcg
     1381 acaccggcga agaccggcgt cagcgtgcaa tcaccggtcg gctgcacctc ggcgcaggac
     1441 gacggcggtg actcgcaggc gccagcggca tccgaggcgg cgatcacacg gtagctgtag
     1501 gtcgccccgc ccgaaacgtc gacgtcgagg taggtcgtgg cggcgacacc ggtcgcgagc
     1561 cggacccacg gcccgtccgg acaggccgcg agcgcgcgtt cgaccgcgta cgaggcggcg
     1621 ccgtcgaccg gcgcccagct cagctgcacc tggccgtccg cgggcgtcgc gagcgcggga
     1681 acggcgggcg cgggcggggc cgcgcactcg tcgaacgctc cgcggacgac cagcgcgtag
     1741 ggctgcggcc cccacggcac cgaaacaccg ctcactcgca cgacccagcg gccggcggcg
     1801 ggggaggcga agaccagctg ctcctcggtg ttgagctcgt ccgcctcgtc ggtcggatcg
     1861 tcggtcgcgg ccggcgtgcc gtcgagcggg tcgagcgtct ggaaccagac gttgcccgtc
     1921 gggtcgacga cctcgaggtg cagctcgttg acccgcgccg tcgccgcgtt cggctcggcc
     1981 gggtagtcgg tccagacgag ggtcgccgcc aacgtctcgc cggccgtcac gtcgatcgcg
     2041 tgttgcacga ggctgccggt cgtcagaccc tggcgatggt cgtcgaccaa cagacggaac
     2101 gaggcgccgt cgaacggcag caccgtgtcg agctgcacgc ggccgaagcc cgagtcgtga
     2161 tcgggacccg cgccggcgat gtcgaccgcg gcgccgatca gcgtcgcctt gagcagcgcg
     2221 ccgctcggcg agatacccgc gcccgcgtcg cgcacgcccg agacgtggaa gcccgccgcg
     2281 tagtactcgc gtaccagcgc cgccagaccg gcgacggtcg gagcggacat cgacgtaccg
     2341 tcgagcgagc aggtcgagca gtcgagcgtg ccgagatcgg cgtcggacgc cgccgagacg
     2401 acggcgcgac cctgggccgc gacggtcggc cgcagccggc cgtcgaacac cggaccgcgg
     2461 ctcgactccg gcatgacggc gttggccgag gcgccgtgct cgagaccgcc gacggcgagg
     2521 acgttcttgg cgatcgccgg cgtgacgacc gagtccggcg gcaggcagag cgtgcccgag
     2581 ttgccggcgg cgttgaccag cagcaggtcg ggataggtcc acatcgcgag gtcggcgtcg
     2641 cgggccagcg agtcgtacgg cagctcgcag cccggcacgc actggccgag cgcgtcgtag
     2701 cagacgccac cgaacgagaa cgagtggata cgcgcgccgc tctggaaggc gacgtcggcg
     2761 atgttccaga tcgaaccgcc cagctcgttg aggtattcga gaccgtcgcc gagctcgatc
     2821 acgacgagcc gcgcgcccgg ggcctgaccg tcgccggcgt tgcgattggg cgagacgaag
     2881 gtcgtacagt cggcccacgg cgcgttgtcg cccgcggccg agcccgaggt gtgcgtaccg
     2941 tggccgctcc cgccgaggaa cagcgtcgga caggtgtcct cctcgccggt cgccgtcgac
     3001 gaccagttgt aatagaggat gtccttgcgc agcgacaggt cgggagtgcc ggccggacat
     3061 ggcggcgtcg cgcagcccag caccggcggc ggaccggcgg gatcgcggaa ctggcaggag
     3121 tcgtagtcct gcgcggtgtc ggccacggag atcacctggc cgcagccgaa gatcccgcgg
     3181 tcgaagatcg gtgtctgctg ggccgccgga ccgacgaacg actggtgcac ccagacgccg
     3241 tcctggttca acgggacgac gcggcggacc ggttcgacgg cgacgacctc ggggagcgcc
     3301 gcgagcgcgg acaagacgac gcgcaggcgc gcggcgggca ccgtggcgct gacgcgccag
     3361 gcgaggtcgg gtccggcggg cgccgcggtc ggcgccagtc cggccaggat cccgagagcg
     3421 cgtccgggca tctcgcccgg ggcgaggatc gcgcgcagtc ggacgtcgcc cgccgggtcc
     3481 gcggccgccg cggcgagcgg ggctgcgatc tcgaggtgcg gcggcgcggc gccgagccag
     3541 gcgacgaacg gtagcgagcg gatcgcgctc tcggcacccg gcgaaacgca cagtcgatag
     3601 ccgttgtccg gcaggtagcc gagcagacgc gcaccggtcg cttcgagacg gtgccgctcg
     3661 gccggctcga gcgcgcgctc ggcgatcgcg accaggcaac gctcgccgcg gggcgcaggc
     3721 gcgctgcggt cggtcgagaa ccacgccggc ggggtttgtg gcgccgccgg acggaactcg
     3781 ccgccggcga agcggagccg cagctcgcca ggggcggcga ctgccgcgga cgtcgcgacc
     3841 gcgaacgaca gccggagcag aaagcggatg cggcttgcgc gcacgtcggt cctcacgggt
     3901 ggcgcggggc gtcgagatca atgtcgtgcg cagcggtcgc gagtcaagcc gccccggttt
     3961 caacatcgaa atcgaggctc cgctcgtcgt cgggcagtcg aaaaatcgga tagaatcccc
     4021 gactcgcgat ggtttcgaat ccgttcatcc gcgcggtccc ggttgtcgcc gcgctcgcgg
     4081 ctctcgtctc ttgcgtcggc ccggcgaccg agaaggaccg catcgtcctg gaacggaccg
     4141 gcgccggcga cggccgctgg cgtgcgacct acctcctcgg cgagccggcc ggcgagctcc
     4201 gcttccggcg ccaggccggc ttctaccgcg aagccgtttg gacgatcgcg agccccggtt
     4261 ggcgtttgga ccgccggggc gacgaccaga cgatcgtggc cggccccgac gcggaccgcc
     4321 gtcgtctggt ggtcgagttt ccccagcaca ccgacgagct cgccaaggag tacgagttct
     4381 tcgccgaatt cagcgacggc agcgtcgccg tctacaccgg gcatctctac ctgacggacg
     4441 agccgccgcc gcgaccgggc gcggaggacg acgccgactt cctcgtgcgc gtcgagctgc
     4501 gtccgcgcga cgacgagcag atcgtcgtgc ggggccggac ggcccgcggt cgcacggtct
     4561 gggacgaccc cgaaggcgac gggacctacg tctacttcgg cgggatcgag cccctcgaga
     4621 cggacgcgat gatcgcggtg atcgaccccg gcgcgccgcc gtggctgcgc gacgagctgg
     4681 gtcgcctgct gcccgagatg ttcgcgcgct acacgaccga gttcggctcg cggctgccgt
     4741 ggaagccggt ggtgctgttc agcttcgacg gcgagggctc gggactctcg agcggtggcg
     4801 ggactctcac tggactggtg cagatgtccg cccggggcag cgcctggtac gatgcctcgc
     4861 cggacgcggc ggagcacctg ctctacctga tcgcccacga ggcggcacac ctgtggaacg
     4921 gccagttcca tccctaccgc gacgactccg acagctggat gcacgagggc agcgccgacg
     4981 cgttcgccga actggcgctc cttcgcagcg ggacgatcga cgccgcccgt ctggcggagc
     5041 gacgcaccga ggcgctaggc cggtgcatcg acgggctgcg cggcgtctcg ctgcgcgagt
     5101 cgggcacgag cggcaggttc gacaactact acggctgcgg caacgtgctc gcgttgtggt
     5161 cggtggcggc actgggcgac gccggcgaac ccgaccggct cttcgacctc tggcgggcgg
     5221 tcttcgccca cgccgacgac gacgggcgct acgaccggca gagctacttc gacgcgctgg
     5281 cgacgctggg cgtcgaggcc gaacgggtcg gcgagttgcg ggagttcctc gacaccgtcc
     5341 atgccgaccc ggcggcggcg atacgcgcgc tcttggcccg cgccggcctc cgcctggtcc
     5401 cgctcgaccg gccggtgccg tcgcggcggc gcgcacgggc caccgcggcc gtcgcgcacc
     5461 tgatgcgcgc cgcgtgcgac ggctggtaca gcttctacac ggagtggccg tcgatccgca
     5521 gcgcgacctc cgagtcctgc gagccgttcc gcgccgagtt gcgcatcgtc cgcctggagg
     5581 ggcacgggat cgccgacgac ggcgaccgtg gttacgacgc ggtggccgag cgttgccgcg
     5641 cagggctccc ggtccggctc gggctcgacg gaggcggcgc ggtgtccgtc ccgtgcgaga
     5701 cggtgctgcc cgccctgctc ccgccgctcg ccgttccgca gctgtgagcc cttgcgggcg
     5761 acctactcga cgacggccaa gcttccgccg cgacgctcga gcgagaacgt gagcaccggt
     5821 cggggcagtt ccgatcgttc ccgcacgggc tcgagacgat cctcgagacc gaggatcgtg
     5881 acgccttcgg gcagccagat ggtgccgccg gcaagggcgg ccccgacgcg gatctcgccg
     5941 tcgcgcaccc accggccgcg caggtcgaga tcgatctcgc cgagatgctg ccttacgctc
     6001 gtccgatcgg ggctcgcgtt gccgagaccg gtgatctcga ccgatccctt gtcgccgacg
     6061 agcgacagcc actccatcgg ttcggccagc ggctcggcga agctcagcga aagcgccccc
     6121 tgttcggccc gcagttcggc cgattcgacg atgagcccac ccagctcgac cgcgccgacc
     6181 ccgcttcgga cctcgccgtt cagcacgagc ggcacgccgc gcggcaagct caaccggagc
     6241 ttcggcaacg gaccgccgag cttgacccgt agcagcgcca gcatcgacga gccgctcggc
     6301 tcgaaccgga tgcgataggt ccagctcccg tcggccgccc gctcgaaccg ctcgtcgagc
     6361 gcgtaccgat tcgggtcgta cctcgtttcg agccggacga actctcccgc cgctgccgga
     6421 aggacggtca gctgggcgac cgagaccgcg agcacgaccc ggccgcgccc cgacggggcc
     6481 gtcgagcgat cgccgtccgc ctccggggcg cgccgcgcgg agcgctgcgg cgggagccga
     6541 tgttcgacgg agtcgcgttc cgctcgctcg gagcggatct gccgcagcgc ggtcagcgac
     6601 acgacgccgc cgacgatcgc ggcgacggcg gcgacggcgc cgagaccgag gcagccgagc
     6661 cagagccacc gccgtccgtt cactcgtcga ttataggcgg cgggcggccg gctaccggca
     6721 ggggtgatga tgctggtcgc tcgccgcccc cgcgttcgtc aagcgacaac gagcacgatc
     6781 aatcgtgatt cgcggtatcc tcggcgtcga tgcgtgtgtt ggtcgtcacc ggcgatcatc
     6841 gtcttcccga ctcgaccaaa tgggacgggg cctacaccga gggcgatctc gagctgcatc
     6901 ggacgatgca ggacgcg
//
LOCUS       DASPGN010000377         6116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1590645, whole genome shotgun sequence.
ACCESSION   DASPGN010000377 DASPGN010000000
VERSION     DASPGN010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590645"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VD788_10820"
     CDS             complement(<1..184)
                     /locus_tag="VD788_10820"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXV76800.1"
                     /translation="MQERDERPDAKVTLLPMVPLRDIVVFPHTMVPFVVGRRPSLLAV
                     ERALETDKRLFLSTQRN"
     gene            complement(238..1479)
                     /gene="clpX"
                     /locus_tag="VD788_10825"
     CDS             complement(238..1479)
                     /gene="clpX"
                     /locus_tag="VD788_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HXV76801.1"
                     /translation="MSRKKSEGEGLKCSFCNKSQRDVRKLIAGPTVYICDECVDICLD
                     IIAEERENEEQEGRLRLPKPVEIKTFLDEYVIGQDSAKKRLSVAVYNHYKRNEIATRR
                     RNEVELQKSNILLIGPTGSGKTLLAQTLARLLSVPFAIVDATTLTEAGYVGEDVENII
                     LKLLQAAGNDVEKCQRGIIYIDEVDKIARKGENPSITRDVSGEGVQQALLKILEGTVA
                     NVPPQGGRKHPHQEFVPVDTTNILFIVGGAFVGLEAIIERRIGRKTMGFKAEVKARRE
                     KDLQQILSKAQPQDLIKYGLIPEFVGRLPVAATLSELDCKSLVKILTEPKNALVKQYQ
                     KIFEFENVSLRFTDDALEAIAAQAVERNIGARGLRMIMEELMLEMMYALPSQSNVKEV
                     VVTKDVVTNRSNPLAVMEKAG"
     gene            complement(1510..2094)
                     /gene="clpP"
                     /locus_tag="VD788_10830"
     CDS             complement(1510..2094)
                     /gene="clpP"
                     /locus_tag="VD788_10830"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HXV76802.1"
                     /translation="MPLVPMVVEQSSRGERAYDIYSRLLKDSIVFIGSPIDDDVANLV
                     IAQMLFLEAEDPDRDVSVYINSPGGTVTAGLAILDTMRFIKPDISTFCVGQAASMAAV
                     LLSAGTKGKRFALPNSRIIIHQPLGGVQGQAADIDIHAREILRMRETLNRILVANTGQ
                     PLEKVAEDTDRDYIMTAARAKEYGLIDEVISERS"
     gene            complement(2098..3375)
                     /gene="tig"
                     /locus_tag="VD788_10835"
     CDS             complement(2098..3375)
                     /gene="tig"
                     /locus_tag="VD788_10835"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXV76803.1"
                     /translation="MKVDLTEQSPVKKVLAFEIDADEMAKETKSVLRGYASKARIPGF
                     RPGKAPLSVIHAKFKKEVEGDVRERVMSRSFHRAAEEHGLRPLGDPVVDEVRAEDGEP
                     FRFKLTVEVLPEIEVKNYRGIEARRESRPVGEREVADALEELRQSRTQFVTEEGRAAM
                     TGDVISCDVRGVPDGGQPFERERTLVEVGAPANPPAFNERLEGARAGEKLSFAVDYPK
                     EYENPDLAGKTVRFEITVHEVKVRQVPQLDDEFAKDLGDFDDLAALTARVRSDLEQRR
                     SREVEGKLRQTLLDKVLLENPMVLPDVLVDSEIRQRLEELIRRLYLQGVDPEKVEIDW
                     KRLRDDQEAPARKSVHARLVLDAIARAEGLAVTPEEIDDRIRNDARSVGESHDQFKKR
                     LREHGGTEVIRTQILREKSLDLLTAVANIQSEE"
     gene            complement(3419..3503)
                     /locus_tag="VD788_10840"
     tRNA            complement(3419..3503)
                     /locus_tag="VD788_10840"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3467..3469),aa:Leu,seq:tag)
     gene            complement(3571..3646)
                     /locus_tag="VD788_10845"
     tRNA            complement(3571..3646)
                     /locus_tag="VD788_10845"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3611..3613),aa:Lys,seq:ctt)
     gene            complement(3694..3770)
                     /locus_tag="VD788_10850"
     tRNA            complement(3694..3770)
                     /locus_tag="VD788_10850"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3734..3736),aa:Arg,seq:tcg)
     gene            complement(3794..3869)
                     /locus_tag="VD788_10855"
     tRNA            complement(3794..3869)
                     /locus_tag="VD788_10855"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3834..3836),aa:His,seq:gtg)
     gene            3932..5230
                     /locus_tag="VD788_10860"
     CDS             3932..5230
                     /locus_tag="VD788_10860"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXV76804.1"
                     /translation="MPRPVPSVLVLLQLLLLAACGWRQAPLQPVFPATRFGVACDGVA
                     DDAPAIQRALDAAGAAGGATVRLPQGTCRLASAAADGSMLEIKGATRLQGAGTGRTVL
                     RVTGAWFGAPVTNMIYTERGASGVVIEDLTLDFNARENRGRFATTGLYGLAVRSSDTV
                     ARRVEVVDSPANGIGVPVAGTRVRFEDCRPHDNRLNGFYAGEVSDIEIVGCEAFGNTN
                     GISLANGSRRVVIAGNRLHHQRADGVSIGDTAPQSHRTDTREVIIAGNRIEDNARAGI
                     ALIASRNRGASPSLQTVVHDNDLLRNAAVAISCNSAQDVRITFNRLEDNADTGVLILN
                     GREIAVYGNTIAGSRLAIRIADNPHGPRPGEIGRISIVGNRFGRSGGLARGLPAVTID
                     LDALTDRVIVADNRIDRPDAITTVDPGADPGQLVRAGNGP"
     gene            complement(5245..5321)
                     /locus_tag="VD788_10865"
     tRNA            complement(5245..5321)
                     /locus_tag="VD788_10865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5285..5287),aa:Arg,seq:tct)
     gene            complement(5339..6007)
                     /locus_tag="VD788_10870"
     CDS             complement(5339..6007)
                     /locus_tag="VD788_10870"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV76805.1"
                     /translation="MSGSKEYFDAIGAGWDRMQQSFFSERIRERAFAIAGVQAGRLAA
                     DLGAGTGFVTGGLLERGLRVIAVDQSPAMLATLRAKFPDADCRGGDAAALPIDDDRVA
                     YCFANMFLHHVDDPPRAIGEMARVTAPGGRIVVTDLDLHQHEFLRVEHHDRWMGFARA
                     DVERWFRAAGLTEVHIACLDERCCACSEAGEQAAIGIFAASGTKPARAVPPIAGVNRV
                     GDPC"
BASE COUNT          979 a         2079 c         1950 g         1108 t
ORIGIN      
        1 cgttgcgctg agtcgagaga aacaaccgtt tgtcggtctc gagcgcccgc tccaccgcca
       61 gcagcgacgg acgtcggccg acgacgaacg gcaccatcgt gtgcgggaag acgacgatgt
      121 cgcgcagcgg caccatcggt aacagcgtta ccttggcgtc aggacgctca tcgcgttcct
      181 gcatctcgag actcctgtgg tgttgcggcc cgagcgaccc gtgagcgaag accgcgacta
      241 gccggccttc tccatcacgg cgagcggatt gctacgattg gtgaccacgt ccttggtgac
      301 gaccacttcc ttgacgttcg actgcgaagg aagcgcgtac atcatctcga gcatcagctc
      361 ttccatgatc atgcgcaagc cgcgcgcacc gatgttgcgc tccaccgcct gcgccgcgat
      421 cgcctccagc gcgtcgtcgg tgaagcgcag cgagacgttc tcgaactcga agatcttctg
      481 atactgcttg accagggcgt tcttgggttc ggtcaggatc ttgaccagcg acttgcagtc
      541 gagctcgctg agcgtggccg cgaccggaag ccgaccgacg aattcgggaa tcagaccgta
      601 cttgatcagg tcctgcggtt gggccttcga caggatctgc tgcaggtctt tctcgcgtcg
      661 cgccttgacc tcggccttga atcccatcgt cttgcggccg atgcgccgct cgatgatcgc
      721 ctcgagaccg acgaacgcgc caccgacgat gaacaggatg ttggtcgtgt cgaccggaac
      781 gaactcttga tgggggtgct tgcgtccacc ctgcggcggg acgttggcca ccgtgccctc
      841 gaggatcttc aacaacgcct gctgcacgcc ctcgcccgac acgtcgcggg tgatcgacgg
      901 gttttcgccc ttgcgcgcga tcttgtccac ctcgtcgatg tagatgatgc cgcgctggca
      961 cttttcgacg tcgttgcccg ccgcctggag cagcttgaga atgatgttct cgacgtcctc
     1021 gccgacgtag ccggcttcgg tcagcgtcgt cgcgtcgacg atggcgaacg gcaccgatag
     1081 caggcgtgcc agcgtctgcg ccagcagggt cttgcccgag cctgtcggtc cgatcagcag
     1141 gatgttcgat ttctgcaact cgacttcgtt gcgccgccgc gtcgcgatct cgttgcgctt
     1201 gtagtggttg tacaccgcga cggacagccg cttcttggcc gagtcttgcc cgatcacgta
     1261 ctcgtcgagg aacgtcttaa tctcgaccgg cttgggcagg cgcagtcggc cttcctgctc
     1321 ttcgttctcc cgctcctcgg cgatgatgtc gaggcagatg tcgacgcatt cgtcacagat
     1381 gtagacggtc gggccggcga tcagttttcg tacgtccctc tgactcttgt tgcagaaaga
     1441 gcacttcaaa ccttcgccct cgctcttctt gcgcgacatc caaccctcga tgcgaacctg
     1501 acagacccgt cagcttctct ccgagatcac ctcgtcgatc agaccgtact ccttcgcccg
     1561 cgcggcggtc atgatgtagt cccggtcggt atcctcggcg accttctcga gcggctgacc
     1621 ggtattggct accaggatac gattcaacgt ctcacgcatc cgcaggattt cacgtgcgtg
     1681 gatatcgata tccgcggctt gcccctggac cccgccgaga ggctggtgga tgataatgcg
     1741 cgaattcggc aaggcgaagc gtttcccctt ggtgcctgcg gacagcagca ccgccgccat
     1801 gctggccgct tggccgacgc agaacgtgct gatatcgggc ttgatgaacc gcatcgtatc
     1861 caggatcgcc aggccggccg tcaccgtacc gcccggagag tttatataca ccgagacgtc
     1921 gcggtcggga tcctccgctt cgagaaagag catctgcgcg atgaccaggt tggcgacgtc
     1981 gtcgtcgatc ggcgaaccta taaatacgat actgtccttg agtagacgcg agtagatgtc
     2041 gtaagcccgc tcgccacggc tgctctgctc gacgaccatg ggaaccagcg gcatcgctca
     2101 ctcctcgctt tgaatattag caacggccgt tagcaagtca agcgacttct ctctaagtat
     2161 ttgggtccta atgacttctg tcccaccgtg ctcgcggaga cgtttcttga actggtcgtg
     2221 gctctcgccg accgatcgag cgtcgttccg gatgcgatcg tcgatctctt ccggagtgac
     2281 cgcgaggccc tcggcgcgcg cgatcgcgtc gaggaccaac cgcgcgtgga ccgacttgcg
     2341 cgccggcgcc tcctgatcgt cacgcagcct cttccagtcg atctcgacct tctccgggtc
     2401 gacgccctgc aggtacaggc gccggatcaa ctcctcgagg cgctggcgga tctccgagtc
     2461 gaccagcacg tccggcagca ccatcggatt ctcgagcagc accttgtcga gcagggtctg
     2521 ccgcagcttg ccttccacct cgcggctccg ccgctgctcg aggtccgagc ggacgcgggc
     2581 ggtcagtgcg gcgaggtcgt cgaaatcgcc cagatccttg gcaaactcgt cgtcgagctg
     2641 cggaacctgc cgcaccttga cctcgtgaac ggtgatctcg aaccgcaccg tcttgcccgc
     2701 gaggtcggga ttctcgtact ccttcgggta gtcgacggcg aacgacagct tctctccggc
     2761 acgggcgcct tcgagcctct cgttgaacgc cggtgggttg gccggagcgc cgacctcgac
     2821 cagcgtacgc tcgcgctcga acggctgacc cccgtccgga acgccgcgca cgtcgcacga
     2881 gatcacgtcg ccggtcatcg ccgcgcgtcc ttcctcggtg acgaactgcg tgcgggactg
     2941 ccggagctct tcgagcgcat cggccacctc gcgctcgccg accgggcgcg attcccggcg
     3001 tgcctcgatc ccgcggtagt tcttgacctc gatctccggc agcacctcga cggtcagctt
     3061 gaagcggaac ggttccccgt cttcggctcg tacctcgtcg acgaccggat cgcccagtgg
     3121 gcgcaagccg tgctcctcgg cggcgcggtg gaacgaacgg ctcatcacgc gctcgcgcac
     3181 gtcgccttcg acttccttct tgaacttggc gtggatcacc gagagcgggg ccttgcccgg
     3241 gcggaatccg gggatgcgcg ccttgctggc gtagccgcgc agcacgcttt tggtctcctt
     3301 cgccatctcg tcggcgtcga tctcgaaggc cagcaccttt ttgaccggac tctgttccgt
     3361 gaggtcgact ttcatcggtc catcctctcg actcgcgtgt cgggttcggg ctcgcgcctg
     3421 gtgcgaaagg ggggattcga acccccacgg gttgccccac cggattctaa gtccggcgcg
     3481 tctgccaatt ccgccacttt cgcggccgac cgatccactt taccatcgcg gaccgccgcg
     3541 ggccgttggt cggacgcttt cggatgaagg tggtgagccg ccagggaatc gaaccccgaa
     3601 cccgcagatt aagagtctgc tgctctgcca attgagctag cggcccaccc gtgccggaat
     3661 cggccgtcga atggtatcac agcgacggcc gtgtggtgcg cccggaggga ctcgaacccc
     3721 cgaccctctg gttcgaagcc agatgctcta tccggctgag ctacaggcgc gtgccgtcga
     3781 cgaagcaagt gcgtggggtg agtgatggga ttcgaaccca cgacagccag atccacagtc
     3841 tggagctcta ccaactgagc tacactcacc atcggtcatg ccgaaccgcg acgccggaca
     3901 accgagcatt atatagaacg cctccggccg tatgccacgc cccgttccgt cggtcctcgt
     3961 gctgttgcag ctgctgctgc tcgccgcctg cggttggcgc caggcaccgc tgcagccggt
     4021 ttttcccgcg acacgcttcg gtgtcgcgtg tgacggcgtc gctgacgatg cgccggcgat
     4081 ccagcgcgcg ctcgacgcgg cgggtgcggc gggcggggcc accgtgcggt tgccgcaagg
     4141 tacttgccgg ctggcgagcg cggcggccga cggctcgatg ctcgagatca agggcgcgac
     4201 gcgattgcag ggcgccggta ccggtcgtac ggtgctgcgg gtcacgggcg cgtggtttgg
     4261 cgcccccgtc acgaacatga tctacaccga gcgcggcgcg tcgggcgtcg tcatcgaaga
     4321 tctcacgctc gatttcaacg cgcgagagaa ccgcgggcga ttcgcgacga ccgggctcta
     4381 cggtctcgcg gtgcgaagca gcgacaccgt cgcgcgccgc gtcgaggtcg tcgattcgcc
     4441 ggccaacggc atcggcgtgc cggtcgccgg cacccgcgta cggttcgagg actgccggcc
     4501 gcacgacaat cggctcaacg gcttctacgc cggcgaggtc tcggacatcg agatcgtcgg
     4561 ctgcgaggcg ttcgggaaca ccaacgggat ctcgttggcc aacggctcgc gtcgtgtggt
     4621 gatcgccggc aaccggttgc accaccagcg ggccgacggc gtgtcgatcg gcgacaccgc
     4681 gccccagtcg caccggacgg acacgcgcga ggtgatcatt gccggtaacc ggatcgagga
     4741 caacgcccgc gccggcatcg cgctgatcgc ctcgcgcaat cggggcgcca gtccgagcct
     4801 gcagaccgtc gtccacgaca acgacctgct gcgcaacgcc gcggtcgcga tctcgtgcaa
     4861 cagcgcccag gacgttcgaa tcaccttcaa ccggttagag gacaatgccg acaccggcgt
     4921 gttgatcctc aacgggcgcg agatcgcggt ttacgggaat acgatcgccg gcagccgcct
     4981 ggcgatccgg atcgccgaca acccgcacgg accgcgaccc ggggagatcg gtcggatctc
     5041 gatcgtcggc aaccggttcg gccggagcgg tgggttggcc cggggactgc cggcggtgac
     5101 gatcgacctc gacgcgctga ccgatcgcgt gatcgtcgcc gacaatcgga tcgaccgtcc
     5161 cgacgcgatc accaccgtcg accccggcgc ggacccgggg cagctggtgc gcgccggcaa
     5221 cggaccctga gcatcgcggg cgggtggtac gcctggaggg attcgaaccc ccgacccgcg
     5281 gcttagaagg ccgctgctct gtccgactga gctacaggcg cccgcggaaa ccgccagttt
     5341 agcagggatc gccgacgcgg ttcacgccgg cgatcggcgg cacggcgcga gcgggtttcg
     5401 tgccggatgc ggcgaagatg ccgatcgccg cttgctcgcc cgcctccgag cacgcacagc
     5461 agcgttcgtc caggcaggcg atgtggacct cggtcaagcc cgccgcgcgg aaccaacgct
     5521 cgacgtccgc gcgcgcgaaa cccatccacc gatcgtgatg ctcgacgcgg agaaactcgt
     5581 gctggtgcag gtcgaggtcg gtgaccacga tccggccgcc aggcgcggtg acccgcgcca
     5641 tttcgccgat cgcgcgcggc gggtcgtcga cgtggtgcag gaacatgttg gcgaagcagt
     5701 acgccacgcg gtcgtcgtcg atcgggaggg ccgccgcgtc gccgccacgg cagtcggcgt
     5761 cggggaactt ggcgcggagc gtggcgagca tcgccggtga ctggtcgacg gcgatcacgc
     5821 ggagaccgcg ctcgagcagc ccgcccgtga cgaagcccgt gccggcgccg aggtcggccg
     5881 ccagtcgccc ggcttggacg ccggcgatgg cgaacgcccg ctcgcggatg cgctcggaaa
     5941 agaagctctg ctgcatgcgg tcccaacccg cgccgatcgc gtcgaagtac tccttgctcc
     6001 cgctcaccgc tcgtcccttc ccttctcgga tcgcgctcgg cgccgcgccc ggcgaaactg
     6061 ggcgcgcacc cgcccgcccg aaacggcgac gacgatcgcc tcgacggctt cgatct
//
LOCUS       DASPGN010000378         3509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_653740, whole genome shotgun sequence.
ACCESSION   DASPGN010000378 DASPGN010000000
VERSION     DASPGN010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3509
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653740"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1783
                     /locus_tag="VD788_10875"
     CDS             293..1783
                     /locus_tag="VD788_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV76806.1"
                     /translation="MIRTGTAAFVAVSILTAAAAAPPPELTVGQVVIRALELSPELAV
                     LRDEPAAFETLAAEERALFDPLLSVALGHERVAGRDLSASGRLFDYNTDSPVAELWLT
                     TLLPIGTTLELGATNLVEDASADGQQLVEARLRASIRQPLLRGVRPSVNRGRVRAARQ
                     EAEGARHEAQAAAATLAAEVEQACWDLILAREQIAVLEAAEAQARELRESVRERVGSG
                     ITARDQIVAAESEIALRRQDLSEARTLLDVSTAVVVGYIGPLDAGGALELRIVDPLAA
                     PAGVIGAVDALVESALRDRPELARARVLAQRGEDEVDRTRGLLLPQVDLWADASLLGY
                     GDTSRQAWDDLDGRYWSVAAGLAFERVLGNRDDRADHRRELVRRRQAVNTVAGLERLV
                     ETEVRVAHAQARRSLDFIAAVTATRALDEQKLVVEQVRFELGQASAFQVARAQRDLVE
                     RRLDELTAIAESHKALVELERAAGRLLERHGLAVEPAPGTTETGPR"
     gene            1780..2961
                     /locus_tag="VD788_10880"
     CDS             1780..2961
                     /locus_tag="VD788_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76807.1"
                     /translation="MTGAGGWRDALRERLRPGMLVQLAVWLSSVGLCTALLVGQTGGA
                     PVVGVARGRIVQLGVAWDSLVEAVPVKLFQSVRRGEVVALLDDSVLEAQIVQVEAEIA
                     RLRAEHEEAQKLLEAEFDEREAGWDADGRSFARDASELAIRLREVQVDLEYDCALRDG
                     LRAQAGSLTRLVERGHAPAVELELARAEAVATERRCEESERLAAELAQRLADAETLRD
                     RHRLHEPARPARDAAVEQLSQAIAVQRGLLQELEAMRAQCILRSPFDGTIVAIARRAG
                     DAALARPGEGEIRQRGEAVRPGDAVVAVAADVASEIVAWVVEGSAHTPQPGARVELIT
                     TGPSPRVTRSSIESVGATVERLPERLWVHQVPVWGRPFVVPVPAGFDVAAGDRVEVRL
                     R"
     gene            3007..3357
                     /gene="ssrA"
                     /locus_tag="VD788_10885"
     tmRNA           3007..3357
                     /gene="ssrA"
                     /locus_tag="VD788_10885"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          490 a         1164 c         1344 g          511 t
ORIGIN      
        1 cttgcccggc aagcgcaggt cgaggacgat caggtcgtag ggcacggaca gcgccttgct
       61 cagtccgctg tcgccgtcgc cggcgaggtc gaccgcgaac gaggcgttct cctcgagcat
      121 ccgccggatg ttcctggcga gccgggtctc gtcttccacg atcagcgcgc gcactcggta
      181 ctctcgtcgt tcgggagaaa cgatacacca cgccccgcgc tcgccacggc gcgggccgcc
      241 ttcagcttcg cttcagccgc tgcacggtag cttgaccggt cgaggacgtg tgttgattcg
      301 caccggcacc gccgccttcg tcgcggtctc gatcctgacc gctgccgccg cggctccccc
      361 gcccgagctg acggtgggcc aggtggtgat ccgtgcgctc gagctgagcc ccgagctggc
      421 ggtgctgcgc gacgagccgg cggcgttcga gaccctggcg gcggaggagc gggcgctgtt
      481 cgacccgctg ctgagcgtgg cgttgggcca cgagcgcgtg gccggtcggg acctcagcgc
      541 gagcggccgg ctgttcgatt acaacacgga cagcccggtg gccgagctgt ggctgacgac
      601 gctgctgccg atcggcacca cgctcgagct gggcgcgacg aacctggtcg aggacgcgag
      661 cgccgacggc cagcagctgg tcgaggcgcg gctgcgggcg tccattcgac aaccgctgct
      721 gcgcggcgtg cgcccgtcgg tcaaccgggg ccgggtccgg gcggcgcgtc aggaagccga
      781 aggcgcgcgt cacgaagcgc aggccgcggc ggcgacgctc gcggccgagg tcgagcaggc
      841 ctgttgggat ctgatcctcg cgcgcgagca gatcgcggtg ctcgaagccg ccgaggcgca
      901 agcgcgcgag ctgcgcgagt cggtccgcga gcgcgtgggg agcgggatca ccgcgcgcga
      961 tcagatcgtc gcggccgaga gcgagatcgc gctgcgccgg caggacctca gcgaagcgcg
     1021 gacgttgctc gacgtgtcga cggcggtcgt cgtgggctac atcggaccgc tcgacgccgg
     1081 cggggcgctc gagcttcgca tcgtcgatcc gttggccgcg ccagccggcg tgatcggtgc
     1141 ggtcgacgcg ctcgtcgagt ccgcgctgcg cgatcgaccg gagctcgcgc gagcgcgggt
     1201 cctcgcgcag cgtggcgagg acgaggtcga ccggacgcgc ggtctgctgt tgccgcaggt
     1261 cgacctgtgg gccgacgcgt cgctgctcgg ctatggggat acgtcacgcc aggcgtggga
     1321 cgatctcgac ggacggtact ggagcgtcgc cgccggcctt gccttcgagc gcgtgctggg
     1381 caaccgcgac gaccgtgcag accatcggcg cgagctcgtg cggcggcgac aggcggtcaa
     1441 caccgtcgcc ggactcgagc gcctggtcga aaccgaagtg cgggtggcac acgcgcaggc
     1501 gcggcggagt ctcgacttca tcgccgcggt caccgccacg cgcgcgctcg acgagcagaa
     1561 gctggttgtc gagcaggtgc gcttcgagct gggccaggcc agtgcgttcc aggtggcgcg
     1621 cgcccagcgc gatctggtcg agagacggct cgacgagctg accgcgatcg ccgagtcgca
     1681 caaggcgctg gtcgagctcg agcgggcggc gggccgcttg ctcgagcgtc acgggctggc
     1741 ggtcgagccc gcgccgggca cgaccgagac gggaccgaga tgacgggcgc gggcggctgg
     1801 cgcgacgcgc tgcgcgagcg gctgcgcccc gggatgctgg tccaactggc ggtgtggctg
     1861 tcgtcggtcg ggttgtgcac cgcgttgctc gtcgggcaga ccggcggcgc tccggtggtc
     1921 ggtgtggccc gcggccgcat cgtccaactc ggggtggcgt gggactcgct cgtcgaggcg
     1981 gtgccggtca agctgttcca gagcgttcgg cgcggagagg tcgtcgcgtt gctcgacgac
     2041 agcgtgctcg aggcgcaaat cgtacaggtc gaagccgaga tcgcgcgcct gcgcgccgag
     2101 cacgaggaag cgcagaagct gctcgaagcc gagttcgacg aacgcgaggc cggctgggac
     2161 gccgacggcc gctcgttcgc ccgcgatgcg tcggagctcg cgatccgcct gcgggaggtg
     2221 caggtcgacc tcgagtacga ttgcgcgttg cgcgacggat tgcgcgcgca ggccgggagc
     2281 ctgacgcggc tggtcgagcg cggtcacgcg ccggccgtcg agctcgagct cgcccgggcc
     2341 gaggcggtgg cgaccgagcg ccgctgcgag gagagcgagc ggctcgccgc cgagctggct
     2401 cagcggctgg ccgacgccga gacgctgcgc gatcgtcatc gtcttcacga gccggcgcgt
     2461 ccggcgcgcg acgcggcggt cgagcagctc tcgcaggcga tcgcggtgca gcgcggcctg
     2521 ctgcaggaac tcgaagcgat gcgcgcgcag tgcatcctgc gttcgccgtt cgacgggacg
     2581 atcgtcgcga tcgccaggcg ggccggcgac gccgccctcg cgcgcccggg cgagggcgag
     2641 atccgtcagc ggggcgaggc ggtgcgcccg ggcgacgccg tcgtcgcggt cgccgctgac
     2701 gtcgcgagcg aaatcgtcgc gtgggtcgtc gagggcagcg cgcacacgcc gcagccgggc
     2761 gcgagggtcg agctgataac gacgggcccg tcgccgcgcg tcacgcgatc gtcgatcgag
     2821 tctgtcggcg cgacggtcga gcgactgccc gagcgtctgt gggtccatca agtaccggtg
     2881 tggggacgcc cgttcgtcgt gcccgtgccg gcgggcttcg acgtggctgc gggggatcgc
     2941 gtcgaggtgc ggttgcgctg aggcgtcggt ccgcccccgc tgctacaatg agaagcgatt
     3001 cccgtcgggg gcgaactgga ttcgacgggg agagtagagg cgagagtagc acgtcgaggg
     3061 tccactcctc gtaaatcagg tgggctaaca atagtcggca ctaacgacta cgcactcgca
     3121 gcttaagctg tgacgtcttc cgggttgccc gtcccgaggc gacccggcga ggcgacgcca
     3181 tatcgggata gcctggtcgc gacgcccgtg gcgaccgggc gaaaatcacg ggctggccac
     3241 cggtcgactt gccgttcagt aggccggtgg cgagataaaa agaagaaccg gctaaacgtg
     3301 tagaagctct ctaccgaacc tttccggacg cgggttcgat tcccgccgcc tccaccattc
     3361 gactcgcgcc tgcggcgctc gctcatggca ggccaggtga tcgcgagtcg aatggtgccc
     3421 tgagcgagcg aagcgagtcg aagggctact tcgctcttga tcggtccccc gccgcttccc
     3481 agggtcggtt ctgtaccacg catacatcc
//
LOCUS       DASPGN010000379         7243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1239214, whole genome shotgun sequence.
ACCESSION   DASPGN010000379 DASPGN010000000
VERSION     DASPGN010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239214"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VD788_10890"
     CDS             complement(<1..692)
                     /locus_tag="VD788_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXV76808.1"
                     /translation="MRSRDYDAVIVGARCAGAATAMLLARRGLRVLAIDRASYGRDTL
                     STNALMRGGVLQLGRWGLLGAVEAAGTPAVRHTAFHYGDEVIDIAIKPRDGIDALYAP
                     RRTVLDALLVDAAREAGATVRFGARFRELLHDAGGRVRAVVIENADGTIDTVGCRIAV
                     GADGVRSTVARAVGAGRYRVARHATGVVYGYFHGLELDGYHWYYRALVSAGAIRTNDD
                     LTCVFASVPANR"
     gene            complement(713..1294)
                     /locus_tag="VD788_10895"
     CDS             complement(713..1294)
                     /locus_tag="VD788_10895"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXV76809.1"
                     /translation="MPDTPVVKSTPAPRNGVDTPTLFATINAVGQQPELAAFTFRASN
                     EWQGGTHSRSTMETFSGAGGDHRHSRTYRYDGDHPQVLCGTDNGPTPVEFLLHGLASC
                     LTAGIANIAAARGVTLSEVKSTIEGQIDLQGILGLSDEVRNGYQRVRVHFTIKGDAPE
                     EKLRQIVEQSRNRSAVFDVLTNGTPVEITVNAA"
     gene            1486..2727
                     /locus_tag="VD788_10900"
     CDS             1486..2727
                     /locus_tag="VD788_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXV76810.1"
                     /translation="MKRTETVIIGAGQAGLAMSRCLVERGVPHVVLERGRIAERWRSE
                     RWDSARLLTPNWQSRLPYCHYRGSDPDGYMKMSEVVDYFESYAGSFAAPVETGTTVLS
                     VEQPRRDVFVVTTDRGVWQTSNVVIATGHCDVPYIPPLARGLSDGIHQTVPTRYRNPD
                     SLPAGGVLVVGASASGTQLASELVAAGRQVTLAVGRHTRLPRQYRGKDIPWWLEMSGK
                     FREPIEPTDDGAARRFRPSTQLAGRTDRGNLDLGVLQARGVRLTGRAIAACGSIVRFA
                     DDLAESVARADDQLQKVLGQIDEFIETTGMSHRVGPRESIERIHPLRAPSEIDLSRER
                     VTTVLWATGFRRNYAWLRIPVLDGDGEIRHRGGITPVAGLYLLGLQSMRRRNSSFIDG
                     VGIDAEELASHLAARVRSRAA"
     gene            complement(2735..3172)
                     /locus_tag="VD788_10905"
     CDS             complement(2735..3172)
                     /locus_tag="VD788_10905"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HXV76811.1"
                     /translation="MSPQPPVWPRGKPSVGQRARLERRVAAEDIERFTEISGDRNPLH
                     YDREAAAATKFGGIIVQGGVTSAILNAVVAEKLPGPGTVFLHVDWSFRAPVRPGDRIA
                     GEVEVLDVRDDKPIAKLATRVVRDDGTVALDGTALCYTMPLPG"
     gene            complement(3186..3311)
                     /locus_tag="VD788_10910"
     CDS             complement(3186..3311)
                     /locus_tag="VD788_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76812.1"
                     /translation="MIEAADDCAAPQLDAILAGVEVTLPDDVGGEHPEAVSSGLQ"
     gene            3778..4344
                     /locus_tag="VD788_10915"
     CDS             3778..4344
                     /locus_tag="VD788_10915"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXV76813.1"
                     /translation="MIAALRRIVRAIDLHSRQLVELFNVTGPQLIALQELARLGPVPV
                     GLLARNVHVSHPTMTGILDRLERRGLALRTKDTADRRRITVHVTRIGRELLEAAPSPL
                     QDRFRAEFAKLEEWEQTQMLSTLQRIAAMMNVEALEASPVLTSDLAVSAATEILPITT
                     PLVIPHTPAPTAGEPSVTAGRDPGEPVR"
     gene            4341..5120
                     /locus_tag="VD788_10920"
     CDS             4341..5120
                     /locus_tag="VD788_10920"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV76814.1"
                     /translation="MTDAANETVSATALALPASGESLEQDSEWCVVDVGGRWRKIRFH
                     DYDEIYSIVGLYEKLFYDVLKCSSPTTIRSMLGRCLRESRVAPGDLAVLDLGAGNGMV
                     GEELAKLGIRRIVGVDIIPAAAAAARRDRGEIYRDYFVIDMTAPPEQAVERLGRHRLN
                     CLTCVAALGFGDIPPRAFANAYNVIERDGWVAFNIKSRFLGNGKPGGFAGLIRRMIGE
                     RLLEVRDERRYTHRLATSGEPLEYTAFVGVKQHDVPAGWEM"
     gene            5138..5605
                     /locus_tag="VD788_10925"
     CDS             5138..5605
                     /locus_tag="VD788_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXV76815.1"
                     /translation="MMTAPKRATLVCLAGGLLLAALPAAGRPRVEFETEGPIITTEGH
                     ATLRWAAVDPVERGSVFELQDSTDPEFRSTHVWYRGPDRASFVSGLPEGTSWFRVRAV
                     RGQRAGPWSDPVAIEVDYASSSTLGRLLALGAVVFLATLVTVVVGHRRREAGG"
     gene            5629..7077
                     /locus_tag="VD788_10930"
     CDS             5629..7077
                     /locus_tag="VD788_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76816.1"
                     /translation="MDLRSAVLDQTTGVLLLAVFGVLWVALGVWWGRNARDLEGFMLA
                     GRNVGLALGSATAMATWVTSNTIMITPKFALQMGVWGMLAYSTASFGLLLFAPMAYRI
                     RSLMPRAFTSGDFIRLRYGRLAWGLFLAISLVYSMTWLVSMGMAGGIVMQALSGIPYL
                     HGMTVILTVCVLYTLFGGLYAVIGTDFIQSVIILIGVVVVGAVVLQKVELSRFHADLM
                     RDHPALLDMLMPVALLSLFNNMFFGFGEIFHNNVWWSRAFAMRDGVPHKAFGLSALFW
                     LPIPVATGFIGLCAGPLGINVPDADMVGPLVASRVLGSAGAIVVFIVVFCSLASSIDS
                     LLAATSDLLVEDVYRKMLRPRSSESHLRRASTWIIVGLGVVTWLLCAPRLGDLIQVLF
                     LSGPLVASAIWPIVAGLYWRGASGLGAVLGMLLGSAVGLWAYFAQGWFVASLISAAVS
                     MTAVATATWLRPSSFVWSALDEHPTSAVAGGR"
     gene            7074..7199
                     /locus_tag="VD788_10935"
     CDS             7074..7199
                     /locus_tag="VD788_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76817.1"
                     /translation="MTVFPEWFFNALILAALILTGLSAAGLLSLLFRDWKKGRLW"
BASE COUNT         1033 a         2470 c         2507 g         1233 t
ORIGIN      
        1 aagcggttcg caggaaccga ggcgaaaacg cacgtcaggt cgtcgttcgt ccggatcgcc
       61 ccggcgctga ccagcgcgcg gtagtaccag tgatagccgt cgagctccag tccgtggaag
      121 tagccgtaga ccacgcccgt cgcgtgacgg gcgacgcggt atcgaccggc gccgaccgca
      181 cgcgcgacgg tggaacgcac gccgtcggcg ccgacggcga tgcggcagcc gacggtgtcg
      241 atcgtgccgt ccgcgttctc gatcacgacg gcgcgcaccc ggccgccggc atcgtgcagt
      301 agctcgcgga accgcgcacc gaagcggacg gtcgctcctg cttcgcgtgc ggcgtcgacc
      361 aacagcgcgt cgagcaccgt gcgtcgcggc gcatagagcg cgtcgatccc gtcgcggggc
      421 ttgatcgcga tgtcgatcac ctcgtcgccg tagtggaagg cggtgtgccg cacggccggg
      481 gtgccggccg cctcgaccgc gccgagcaac ccccaacggc cgagctgaag cacgccgccg
      541 cgcatcagcg cgttcgtcga cagcgtatcg cggccgtacg aggcgcggtc gatcgccagc
      601 acccgcagcc cccggcgggc cagcagcatc gccgtggccg cgccggcgca gcgggcgccg
      661 acgatcaccg cgtcgtagtc acgagagcgc atcgcgtcga ctcgcatccg gatcaagccg
      721 cgttgacggt gatctcgacc ggcgtgccgt tggtcagcac gtcgaacacc gccgaacggt
      781 tgcgcgactg ctcgacgatc tgccgcagct tctcctccgg cgcatcgccc ttgatcgtga
      841 agtggacgcg gactcgctgg tagccgttgc gcacctcgtc cgacagaccg aggatgccct
      901 gcaggtcgat ctggccctcg atcgtcgact tgacctcgct caacgtcacc ccgcgcgcgg
      961 cggcgatgtt ggcgattccg gccgtcaagc acgaggccaa accgtgcagc aggaactcga
     1021 ccggcgtggg gccgttgtcg gtgccgcaca gcacctgcgg gtggtcgccg tcgtagcggt
     1081 aggtgcggct gtgccggtgg tcgccgccgg cgccgctgaa ggtctccatc gtgctgcgac
     1141 tgtgggttcc gccctgccac tcgttgctcg ccctgaaggt gaacgcggcc agctcgggct
     1201 gctgcccgac ggcgttgatc gtggcgaaca gggtcggcgt gtcgacgccg ttgcgcgggg
     1261 cgggggtgga cttgactacg ggggtgtcag gcatgtcgtg ctcctttgac gggtgttcga
     1321 tcccggcttt gccttcaccc cgaggaaacg cgaaaccgcg cgcggccccg ccacccgccc
     1381 ggcgtttttt ttcgggggat cgaccgaagc tggccgggtt ggcgggattc ctcccggttt
     1441 ggcgtaaccc gggtggaacg acgaaccgac gaggagtgcc caccgatgaa acgcaccgag
     1501 accgtcatta tcggggccgg ccaggccggg ttggcgatga gccgctgtct cgtcgagcgc
     1561 ggcgtgcccc acgtcgtgct cgagcgcggc cgcattgccg agcgctggcg cagcgaacgc
     1621 tgggattcgg cgcgactgct gacgccgaac tggcagagcc ggctgcccta ttgccactat
     1681 cgcggctccg acccggacgg ctacatgaag atgagcgagg tcgtcgacta cttcgagtcc
     1741 tacgccgggt cgttcgcggc gccggtcgag accgggacga ccgtgctgtc cgtcgagcaa
     1801 ccgcgccgcg acgtgttcgt ggtgacgacc gaccgcggcg tgtggcagac gtcgaacgtc
     1861 gtgatcgcca ccggccactg cgacgtaccc tacatcccgc cgctggcgcg aggactgagc
     1921 gacgggatcc atcagacggt gccgacgcgc taccgcaatc ccgactcgct gcccgcgggc
     1981 ggcgtgctgg tcgtcggtgc gtcggcctcg ggcacccagc tcgcgagcga gctcgtggcc
     2041 gcgggtcgcc aggtgaccct cgcggtcggc cgccacacgc gcctcccgcg gcagtaccgc
     2101 ggcaaggaca tcccctggtg gctcgagatg tccggcaagt tccgcgagcc gatcgaaccg
     2161 accgacgacg gcgccgcgcg gcgcttccgt ccgtcgacgc agctcgccgg gcgtaccgac
     2221 cgcggcaacc tcgacctggg cgtgctgcag gcacgcggtg tgcgcctgac gggacgcgcg
     2281 atcgccgcat gcggctcgat cgtgcgcttc gccgacgacc tggccgagtc ggtcgctcgc
     2341 gccgacgacc aactgcagaa ggtgctgggc cagatcgacg agttcatcga gacgaccggc
     2401 atgagccacc gtgtcggacc gcgcgagtcg atcgagcgca ttcacccgct cagggcgccg
     2461 agcgagatcg atctgtcgcg cgaacgggtc acgacggtgc tgtgggccac gggcttccgc
     2521 cggaactacg cctggctgcg gatcccggtg ctcgacggcg acggcgagat ccgccatcgc
     2581 ggcggcatca cgccggtcgc gggcctctac ctgctcggct tgcagtcgat gcgccggcgc
     2641 aactcgagct tcatcgacgg cgtggggatc gacgccgaag agctggcatc gcacttggcg
     2701 gctcgcgtgc gctcgcgcgc cgcctgagcg gggatcagcc gggtagcggc atcgtgtagc
     2761 acagcgccgt cccgtcgagc gccacggtgc cgtcgtcgcg gacgacgcgg gtcgcgagct
     2821 tcgcgatcgg cttgtcgtcg cgcacgtcga gcacctcgac ctcgccggcg atccggtccc
     2881 ccgggcggac cggcgcgcgg aagctccagt cgacgtgcag gaagaccgtg cccggtcccg
     2941 gcagcttctc cgcgacgacc gcgttgagta tcgcgctcgt cacgccgccc tggacgatga
     3001 tcccaccgaa cttcgtcgcc gcggcggcct cgcggtcgta gtgcaacggg ttgcggtcgc
     3061 cgctgatctc ggtgaaacgc tcgatatcct cggcggcgac gcggcgctcg aggcgggctc
     3121 gctggccgac cgacggcttg ccgcgcggcc agactggcgg ttgagggctc atcgtgcctc
     3181 cgcgttcatt gtagaccgct cgacaccgct tcgggatgct ccccgcctac gtcgtccggg
     3241 agggtgacct cgacgccggc gaggatcgcg tcgagctggg gagcggcgca gtcgtcggcc
     3301 gcttcgatca cgcatcgcgc gtggcgaacg ggttcggcgc gacgccgatc cgtaacatcg
     3361 tgggccggac tgccgcggcg tcgccgccgg ccaggacgcc aagtcggtac agttgcgcga
     3421 gtgctcaagc aggtcgtcgc cgaggactcg tcggcaccgg gcgcacatca agaccagaag
     3481 ctgggctcga gaacacgaac gaactgctgc gctacgcggt cgcctgggcg ctagatccgt
     3541 gaaccgccga gtccagggcg gccgtaaatt taattagcat gagaaatatt caggcctcaa
     3601 cacgactcgt tcggcagctc aagattcatt gcgaaattcc ttttaaacta cgctaaatga
     3661 acaaattaga ttgagtcgtc catcatgctg acttgacttt tttatttctt tgtattctaa
     3721 tctgtttttt gctcggagat cgatcagttg attccatacc ccacgacgga tgatcgggtg
     3781 atcgcggcgc ttcggcgaat cgtgcgggcg atcgacctgc attcgcgcca gctggtcgag
     3841 ctgttcaacg tgaccgggcc ccagctgatc gcgctgcagg agcttgcccg gttgggcccg
     3901 gtcccggtcg gtctgctcgc gcgcaacgtc cacgtcagcc acccgacgat gaccgggatc
     3961 ctcgaccgct tggaacgacg cgggctggcg ctgcgaacga aggacaccgc ggaccggcgg
     4021 cgcatcacgg tccacgtcac gcgcatcggg cgcgagctgc tcgaggcggc gccgtcgccg
     4081 ctccaggacc gcttccgcgc cgagttcgcc aagctcgagg agtgggagca gacgcagatg
     4141 ctgtcgacgc tccaacgcat cgccgcgatg atgaacgtcg aggcgctcga agcgtcgccg
     4201 gtcctgacct cggacctggc cgtttccgcc gctaccgaga ttctaccgat cacgaccccg
     4261 ctcgtgattc ctcacacccc ggccccgacc gccggtgagc cgagcgtcac cgccgggcga
     4321 gatccgggag aaccggtacg atgaccgacg ccgccaacga aaccgtctcc gccaccgccc
     4381 tcgccctgcc tgcgtcgggc gaatcgctcg aacaggacag cgagtggtgc gtcgtcgacg
     4441 tgggcgggcg gtggcgcaag atccgctttc acgactacga cgagatctat tcgatcgtag
     4501 gcctgtacga gaagctgttc tacgacgtgc tcaaatgcag ctcgccgacg accatccgtt
     4561 cgatgctcgg gcgctgcctg cgcgagtccc gcgtcgcgcc gggcgacctc gcggtgctcg
     4621 acctgggcgc gggcaacggg atggtcggcg aggagctggc caagctcggc atccgccgca
     4681 tcgtcggcgt cgacatcatc ccggcggcgg ccgcggccgc ccggcgcgac cgcggagaga
     4741 tctaccgcga ctacttcgtg atcgacatga ccgcgcctcc ggagcaggcc gtcgagcgac
     4801 tcggccgcca ccggctgaac tgcttgacct gtgtcgctgc cctcggcttc ggcgacatcc
     4861 cgccccgggc cttcgccaac gcctacaacg tcatcgaacg ggacgggtgg gtcgcgttca
     4921 acatcaagag ccggtttctc ggtaacggca agcccggcgg cttcgccggt ctgattcggc
     4981 gcatgatcgg cgaacggctg ctcgaggttc gcgacgagag gcgctacacc caccgcctgg
     5041 ccaccagcgg cgagccgctc gagtacaccg cgttcgtcgg cgtcaagcag cacgacgttc
     5101 cggcgggctg ggagatgtga tccgtccggg gagcccgatg atgacggcac cgaagcgagc
     5161 gacgctcgtt tgtctcgccg gcgggctgct gttggccgcg ctgccggcgg cgggccggcc
     5221 ccgcgtcgag ttcgaaaccg agggaccgat catcacgacc gaagggcacg cgacgttgcg
     5281 ctgggcggcc gtcgatccgg tcgagcgggg ttcggtcttc gagctgcagg actcgaccga
     5341 tcccgagttc cgctcgaccc acgtctggta tcgcggtccg gatcgcgcgt cgttcgtatc
     5401 ggggctcccc gaaggcacca gctggttccg cgtccgcgcg gtgcgcggcc agcgagccgg
     5461 accatggtcg gacccggtcg cgatcgaggt cgattacgca tcgagctcga cgctcggccg
     5521 tctgctggcg ctaggcgccg tcgtcttcct cgccacgctg gtgaccgtcg tggtcggcca
     5581 ccgccggcgc gaggcgggcg gctgacgccc gccggtcacc ccagcgcgat ggacctccgt
     5641 tccgcagtcc tcgatcagac aaccggcgtg ctgctgctcg ccgtcttcgg cgtgctgtgg
     5701 gtcgcgctcg gcgtctggtg gggacgcaac gcgcgggatc tcgaggggtt catgctcgcc
     5761 ggccgcaacg tcggcctggc cctcggctcg gcgacggcga tggcgacgtg ggtcacctcg
     5821 aacacgatca tgatcacccc gaagttcgcg ttgcagatgg gcgtctgggg catgctggcc
     5881 tattcgacgg cgagcttcgg tctgctgctg ttcgcgccga tggcctaccg catccgctcg
     5941 ctgatgccgc gggccttcac ctcgggcgat ttcatccgtt tgcgctacgg gcgattggcc
     6001 tggggtttgt tcctcgcgat ctcgctcgtc tactcgatga cctggctggt cagcatggga
     6061 atggccggcg ggatcgtgat gcaggcgctg tccggcattc cgtacctcca cggcatgacg
     6121 gtgatcctca ccgtctgcgt gctgtacacg ctgttcggcg ggttgtacgc ggtgatcggc
     6181 accgacttca tccagagcgt gatcatcctg atcggcgtgg tggtcgtcgg cgcggtcgtg
     6241 ctgcagaagg tcgagctgtc gcgcttccat gccgatctga tgcgggacca cccggcgttg
     6301 ctcgacatgc ttatgccggt ggcgctgctg tcgctgttca acaacatgtt cttcgggttc
     6361 ggcgagatct tccacaacaa cgtctggtgg tcgcgggcct tcgccatgcg ggacggcgtg
     6421 ccgcacaagg cgttcggcct gtcggcgttg ttctggctgc cgatcccggt ggccaccggc
     6481 ttcatcggcc tgtgcgccgg gccgctcggg atcaacgtgc cggacgcgga catggtcggg
     6541 ccgctggtgg cgagtcgggt gctgggctcg gccggcgcga tcgtggtgtt catcgtcgtt
     6601 ttctgctcgc tggcgtcgag catcgacagc ctgctcgccg cgacgtccga cctcttggtc
     6661 gaggacgtct accgcaagat gctccggccg aggtcgtccg agtcgcacct caggcgggcc
     6721 tcgacctgga tcatcgtcgg actcggggtg gtgacctggc tcctctgcgc gccgcggctc
     6781 ggcgacctga tccaagtgct gttcctgtcc ggtccgctgg tggcgagcgc gatctggccg
     6841 atcgtcgccg ggctgtattg gcgcggcgcc agcgggctcg gcgcggtgct cggcatgctg
     6901 ctcggcagcg ccgtcggcct gtgggcctat ttcgcacagg gctggtttgt cgcctcgctg
     6961 atctcggccg cggtgtcgat gaccgccgtc gcgacggcga cctggctccg cccgtcgagc
     7021 ttcgtctggt cggcgctcga cgagcacccg acgagcgcgg tcgcgggagg taggtgaccg
     7081 tgttccccga gtggttcttc aacgcgttga tcctcgcggc gctgatcctg accgggctgt
     7141 ccgccgccgg gctgctgtcc ctgctgttcc gcgactggaa gaaagggagg ctgtggtgat
     7201 gaacgaggca ctggagccca accgcatcac gacggcgctc gag
//
LOCUS       DASPGN010000380        10517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1472779, whole genome shotgun sequence.
ACCESSION   DASPGN010000380 DASPGN010000000
VERSION     DASPGN010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10517
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472779"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VD788_10940"
     CDS             complement(<1..618)
                     /locus_tag="VD788_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76818.1"
                     /translation="MPAVTPHSTYSHRLAAHRRTIARSVAAEGRLAWIRLFLFVVAVA
                     LAWPALFADRLPWWVVLLPVAAFLPVVVWHSRLIQRRERVERAAAFYRRGLDRLDDRW
                     AGGGSAGESFRQPDHPYADDLDLFGRGSLFELLSIAGSGAGERTLAGWLLVGADPETV
                     RRRPAAVAELRPMLDLRERLVVAGADVRAGVDPRQLVSWVGEPAVL"
     gene            complement(673..1029)
                     /locus_tag="VD788_10945"
     CDS             complement(673..1029)
                     /locus_tag="VD788_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76819.1"
                     /translation="MKGLRDATLLLGLLLVLTSVKVGPVDGDADLLPVSHAGSVPAAA
                     SPLVEQIPTGSVIEPTDGAVAAGSPDGEPSCRVSVRTVERQGTVVVRVRGVGEPVFVR
                     PADRPADGSLWACEKG"
     gene            complement(1205..2638)
                     /locus_tag="VD788_10950"
     CDS             complement(1205..2638)
                     /locus_tag="VD788_10950"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV76820.1"
                     /translation="MSAPARTTRARLLVVDDEEPQRLMLERILARAGYDVATAAHGKQ
                     ALELLERQRFDALLTDQRMPEMDGLDLLRHVQRLDAGLPVVLMTAYGTVSNAVDAMKR
                     GAADYLTKPFERDELLVVIDKVLRQRRLEDEVVTLRGALRARYRLDNLVGASAPMQEV
                     FTLIERVSATDVPVLITGESGTGKELVARAIHQQSGRESGPFVALNCAAIPETLLESE
                     FFGHERGAFTGATRTHIGRFEQADRGTLFLDEIGAMRVDLQAKLLRAIQEGEVQRLGS
                     AVSRKVDVRIVAASCEDLEQAIRNRRFREDLYYRLNVVPVRLPPLRERIDDVPLLVDH
                     FVSKAADKFGREGLGVGPEVVDRLQRHPWPGNVRELENCIERMVVLTRAKRLTLQDVP
                     PRIGDAQAATDNVDGFDLPLEGIRLPELERHLIRQALARSRGSLGPAARLLGISYKTL
                     QYRMRKFELDRGAFDGGPAPSPVGRPK"
     gene            complement(2635..4230)
                     /locus_tag="VD788_10955"
     CDS             complement(2635..4230)
                     /locus_tag="VD788_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV76821.1"
                     /translation="MTLRLVALMSVVLLTSLAAFGLMMNHYQDSVMDEVAHTASEVGR
                     ATLRTLEARGSAHAGQAQVLVAETISWNDAVGPIGDGAVDSNARVIFERVGAPARVNL
                     LGERPGGAPSAEATDVERAEFAIVCRSDGQPMTEGDCVSLADAGRRELENQFFIDIDG
                     VRAEADPREGLVLRIETLKPESTEARPPTFVFKRHEDIRLPIPIEEYDSLFDRVRRRS
                     LWVFLGVFALGSVLTAGLASRFTRPIRRLDAGIRRLSSGDLDVQVPTRGNDEIARLGR
                     AFNDMTRSLRANRQRAREMLRREKHSALGRLAAGVAHDIRNPLHSIGLTLEHLKDSCR
                     PETGERADEFERSIEIIRGEIRRLDQIVGSFLGFARNEGRPRHTVALNALLIETARLV
                     RKEAEWRKVDVAVETDPSDPTVGADLDALRSSLLNLVLNSFEAMPDGGRLRLSIDSSE
                     ERVAIEVVDDGPGVPAEDQDRVFDFDYTTKDGGNGLGLAMVYQCIVEDHGGQVTLDSP
                     PDGGTRVRMTLPRVSAGRAEPRA"
     gene            complement(4335..4943)
                     /locus_tag="VD788_10960"
     CDS             complement(4335..4943)
                     /locus_tag="VD788_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76822.1"
                     /translation="MMRARPITARKPRRAVLPALGLLLVGLSAGCGGDDSGGGGGGIV
                     TTPCVELANAVDPVPGTVVAVDGLSGDCDELWVDVTISGVGQPLFGVSFDLLFPTNLV
                     ALRAPDTSGSVLGSGGVPVEIITDPIPGGTNISLARFSSTAAGVDVSGEHLLIRLKFI
                     RFAGSGSGTVEFANPALFDDAEPPAEIPGVTWNGATIRIIQI"
     gene            complement(4975..7650)
                     /locus_tag="VD788_10965"
     CDS             complement(4975..7650)
                     /locus_tag="VD788_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXV76823.1"
                     /translation="MKRVVELTMLALGVIAGAAAVPIAAQGECLGFVERPVVDGGTNG
                     VERLVVADIDRDGRPDLVTASFGDGKLFWYENRSDQPTPPRFLPHLIADDLGSASDVV
                     AVDLDGDQDLDLIAASRERGVFWFEQAAGSQPPVFSKRTVTLETELALGVHAADVDGD
                     GHVDVLTASFLDDTIAWYRNSGTTPIEFTRFVITERALGAAAVWAADVDGDTRTDVVA
                     ASSLDGEVVLYRQTAGAPGTIPAFDKIVLAGLDGRCSVSTDVRCDIAADCPPPAEPMD
                     QPETCVVQPVSSAIGASSVTVADLDDDGRLDVAAAALNENRIIWYRNDGGQPPAFSEF
                     DVTNDAQLVRVVRVADVNLDQKPDLLSASYGDDTIAWYEYVGGQPPGFNEIPVSTDVL
                     SADDVGAADLDGDGAVDIVSVSSVAGFPATRDRIAWYRNNGSGFDEGSTISRSADGAV
                     RAIGADLDGDGRVDVVTAGRSDRLAWYRNDGGDPPSFTEQIVAEDVLGAIDVVAVDLD
                     GDTDLELVVASADDGRIRAFDNGLITGAVRGAPVPVFTEASVDVAAGLDNLQKIVAVD
                     LDGDGDLDLISAEAGEAPEVEEDEPTPPFGRIQWYENDGGGGFSPPQLITDDAGGARS
                     VHAVDLDEDGDVDVLAALFGIDRVVWLENVPDADGPAFPLTREFDVGLLGPTSIDTAD
                     FDGDGDIDIVVSASESEVVALLSSNLDDDPEQPPPSPPAFATRVLATSADGISEVFAA
                     DLDAVPDGDPDIVATIPGDDVIVWFDNDGAAIFTVDLVATEIDFASSSDVADLDGDGM
                     PDIVSGQRLAVLWYQAGGETCQGFDASGDGRIDGVELAYIGRGFGRAVGPASPQWMIA
                     VDYNADGTIDGQDLAILASDGVFGFTTDSCSYVCN"
     gene            7856..8536
                     /locus_tag="VD788_10970"
     CDS             7856..8536
                     /locus_tag="VD788_10970"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV76824.1"
                     /translation="MLRQAFHPIRVTVLAVVAFAPHVAVAERLDDPAALTEQAPDRFS
                     VEFETTKGKFVVDVTRAWAPLGADRFYNLVKHGFYDDVRFFRVVPDFVVQFGLNGDPA
                     IGKPWQSARIKDDPVTRSNEAGTLTFAMAGPNSRTTQVFINLKSNKSLDQSGFSPFGE
                     VSAGMDVVRKLYAGYGDQPPRGRGPNQSQIAMEGNEYLDRSFPKLDAIVDATLIENET
                     ADEAAGGD"
     gene            8529..8807
                     /locus_tag="VD788_10975"
     CDS             8529..8807
                     /locus_tag="VD788_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76825.1"
                     /translation="MTDRDPGGARDVIDFRIDVECYAGHRGEETPRRFAVGRATIEVA
                     DVVDRWLAPEHRYFKVRGDDGGLYILRHDPVAGSWSLTMFESAALRDA"
     gene            complement(8836..>10517)
                     /locus_tag="VD788_10980"
     CDS             complement(8836..>10517)
                     /locus_tag="VD788_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXV76826.1"
                     /translation="GCYGGESARTPVIDGLAARGARFDQAICGAPVTLPSHATIMTGL
                     DVPGHGVRHNGTYRLGDEAWTLAEALAREGYATGAFVGAFVLDARYGLAQGFDHYDDA
                     VHDLAATPSMGRYNERPANEVVDAAIGWLERTIAVDPRRPFLAWVHLFDPHMPYEPPT
                     EYAAMLPGRPYDREIAFCDAQIGRLLEFLAQADLLERTLVVLTADHGEGLGDHDELTH
                     GVLIYDSTVRVPLIVSHPRLFARGVDIADRVAGGTDVFPTVLGLLGVAAGQAEVDGRD
                     LFAAAVDDDRAIYVESLVPLLDYGWSPLRGLRRLGDKLIRAPEPEYYRVTDDPGETVD
                     LYDDSPAAGALEARLDARLAGWPDDAAAVDRQRALDEEELERLASLGYLRAPPSGAAI
                     GAQDPKRMMPLWDKIDRAGKLSLAGDHDGAAELIGEVLDADPASGKAWYTALRIYDRA
                     GRHDAAEQAVRRALALSPSADGWVILARYALNRRDLAEFDGALAEARKLDARHGGIHI
                     GLGHRLAMEGRLAEARAEFARAIEVDPSRSGAHAREQIERIDRIAGRPDAD"
BASE COUNT         1578 a         3731 c         3658 g         1550 t
ORIGIN      
        1 gagcacggcc ggttcgccga cccagctcac gagctgacgc ggatcgaccc cggcgcggac
       61 atcggctccg gcgaccacca gccgttcgcg aaggtcgagc atcggccgca gctcggcgac
      121 ggcggccggc cgccgccgaa cggtttccgg atctgccccg acgaggagcc agcccgccag
      181 cgtgcgttca cccgccccgc tgccggcgat cgacagcagc tcgaacagcg agccacggcc
      241 gaacagatcc aggtcgtcgg cgtaagggtg gtccggttgg cgaaacgact ccccggccga
      301 gccaccgccg gcccagcggt cgtcgagccg gtcgaggccc cggcggtaga atgccgcggc
      361 ccgctcgacg cgctcgcgcc gctggatcaa cctcgaatgc cagacgacga ccgggaggaa
      421 cgccgcgacg ggcagcagca cgacccacca cggaagccgg tcggcgaaca gagccggcca
      481 ggccagcgcg acggcgacga cgaacaggaa tagacggatc caggcgagcc ggccctcggc
      541 cgccaccgag cgcgcgatcg tgcggcgatg ggcggcgagg cggtggctgt aggtgctgtg
      601 cggggttacg gccggcacgg atccctcggc gtggagggct agggtatcag ccgcgccggc
      661 gaatgcctcc ggtcaaccct tctcgcacgc ccacagcgag ccatcggccg gccgatccgc
      721 cggtcgaacg aagacgggct cgccgacgcc ccgcacgcgc acgacgaccg tcccctgccg
      781 ctcgacggtt cgcacggata cccggcaact cggttccccg tcggggctcc cggccgcgac
      841 ggccccgtcg gtcggctcga tcacggagcc ggtcggaatt tgctcgacga ggggcgaggc
      901 ggccgccggc accgagccgg cgtgggacac ggggagcagg tcggcgtcgc cgtcgaccgg
      961 accgaccttg accgaggtca gaacgagcag caatcccaac aacagcgtcg cgtcccgcag
     1021 gcccttcatg ctctctccgt cccgtctgtg tcacccgtag taacgaacga ggcggggctt
     1081 tggatgcgtt ttttttcggc agagcggtct tcgtgcattc ggcgtgccac gtcccgggcg
     1141 gtatcgggcg cggtcgcccg tccaggcagg tgccaccgac tgccccattt ggggcggtgc
     1201 cgctctattt ggggcgtccg accggtgagg gcgccgggcc gccgtcgaat gccccgcgat
     1261 cgagctcgaa cttgcgcatg cggtactgca gtgtcttgta ggagataccc agcaggcggg
     1321 cggcgggacc gaggctacca cggctgcggg cgagagcctg ccggatcaga tgccgttcga
     1381 gctcgggcag ccggatgccc tcgagcggga ggtcgaagcc gtcgacgttg tccgtcgccg
     1441 cctgcgcgtc gccgatgcga ggcgggacgt cttggagcgt cagacgtttg gcgcgggtca
     1501 gcacgaccat tcgctcgatg cagttctcga gctcgcggac gttgcccggc cacgggtggc
     1561 gctgtagtcg gtcgacgacc tcgggcccga cgccgagccc ttcgcgaccg aacttgtcgg
     1621 cggccttcga cacgaagtgg tcgacgagca gcggcacgtc gtcgatgcgc tctcgcagcg
     1681 ggggcagacg gaccggcacc acgttgaggc ggtagtagag gtcctcgcgg aacctgcggt
     1741 tgcggatcgc ctgctcgagg tcctcacagc tcgcggcgac gatccgcaca tcgaccttgc
     1801 gcgagaccgc cgagccgagg cgttgcacct ctccctcctg gatcgcgcgc agcagcttgg
     1861 cttgcaggtc gacccgcatc gccccgatct cgtcgaggaa cagcgttcca cggtcggcct
     1921 gctcgaagcg tccgatgtgc gtgcgcgtgg ctccggtgaa cgcgccccgc tcgtggccga
     1981 agaactcgct ttcgagcagc gtttccggaa tcgccgcaca gttcaacgcg acgaacggac
     2041 cggactcgcg gccgctttgc tggtggattg cccgcgcgac cagctccttg ccggtcccgc
     2101 tctccccggt gatcagcacc ggcacgtcgg tcgcggagac gcgctcgatc agcgtgaaca
     2161 cctcctgcat cggcgccgac gccccgacca ggttgtccag ccggtagcgc gcccgcagcg
     2221 cgccgcgcag ggtgacgacc tcgtcctcga gccgccgctg gcgcagcact ttgtcgatga
     2281 cgaccagcag ctcgtcccgc tcgaacggct tggtcaggta atcggcggcg ccgcgcttca
     2341 tcgcgtcgac ggcattcgag acggtgccgt aggccgtcat cagcaccacc ggcaggccgg
     2401 cgtcgagccg ctgcacgtgc cgcaacaggt cgagcccgtc catctccggc atccgctggt
     2461 cggtcagcag cgcgtcgaac cgctgccgct cgagcagctc gagcgcctgc ttgccatggg
     2521 ccgcggtggc gacgtcgtag cccgctcgag caaggatgcg ctcgagcatc agccgctgcg
     2581 gctcctcgtc gtcgacgacg agcaggcggg cgcgcgtcgt tctcgcgggc gcgctcatgc
     2641 ccggggctcc gcacgccccg cactgacgcg cggcagcgtc atccgcaccc gtgttccgcc
     2701 gtccggcggg ctgtcgagcg tgacctgacc accgtggtcc tcgacgatgc actgatagac
     2761 catggccaga ccgagaccgt tcccgccgtc tttggtcgtg tagtcgaagt cgaagacgcg
     2821 gtcctggtcc tcggccggca cgccggggcc gtcgtcgacc acctcgatcg cgacgcgctc
     2881 ttcgctcgag tcgatcgaca gccgcagccg gccgccgtcg ggcatcgcct cgaagctgtt
     2941 gagcacgaga ttgagcagcg acgaacgcag cgcgtcgagg tcggccccga ccgtcggatc
     3001 gctcgggtcg gtctcgaccg cgacgtcgac cttgcgccac tcggcctcct tgcgcacgag
     3061 ccgcgcggtt tcgatcagca acgcgttgag cgcgacggtg tgccgcggac gtccctcgtt
     3121 gcgggcgaag ccgaggaagc tgccgacgat ctgatccagg cgtcggatct cgccgcggat
     3181 gatctcgatc gagcgctcga actcgtcggc gcgctcaccc gtctcggggc ggcagctgtc
     3241 cttcaaatgc tcgagcgtca gcccgatcga gtgcaggggg ttgcggatgt cgtgggccac
     3301 gccggccgcc agccggccga gcgccgagtg cttctcacgg cgcagcatct cccgcgcccg
     3361 ctgccggttg gcacgcaggc tgcgcgtcat gtcgttgaac gcgcggccga gccgggcgat
     3421 ctcgtcgttg ccccgcgtcg gcacctgcac gtcgaggtcg cccgaggaca gccggcggat
     3481 gccggcgtcg agccggcgga tcggccgcgt gaagcgcgag gcgagcccgg cggtcagcac
     3541 cgatccgagt gcgaagacgc cgaggaacac ccacagcgat ctgcggcgga cccggtcgaa
     3601 caacgagtcg tactcctcga tcgggatcgg cagtcggatg tcctcgtgcc gcttgaagac
     3661 gaaggtcggc gggcgagctt cggtcgactc cggcttgagc gtttcgatcc tcagcaccag
     3721 accctctctc gggtcggctt cggcacgcac cccgtcgatg tcgatgaaga actggttctc
     3781 gagctcccgg cgtccggcgt cggccagcga gacgcagtca ccctcggtca tcggctggcc
     3841 gtcggaccgg cagacgatcg cgaactcggc gcgttcgaca tcggtcgcct ccgcggacgg
     3901 cgcgccgccc ggtcgctcgc cgagcagatt gacccgcgcc ggtgcgccga cgcgctcgaa
     3961 gatcacccgc gcgttcgaat cgaccgcgcc gtcgccgatc ggaccgacgg cgtcgttcca
     4021 ggagatcgtc tcggcgacga ggacttgcgc ctggccggca tgggccgagc cgcgggcctc
     4081 gagcgtccgc aacgtggccc ggccgacctc ggaggcggtg tgtgccacct cgtccatcac
     4141 gctgtcttgg tagtggttca tcatcaggcc gaacgccgcc agtgaggtca gcaacacgac
     4201 gctcatcagc gccaccaaac gcaaggtcat gactctgctc ccgggacggg cgaagcggac
     4261 gacccggccc tggcgtcccg gtcggacgcc ggatccgacg cggaccctgg caagaagtgt
     4321 accagcgcgg tcggtcagat ctggatgatg cggatcgtcg cgccgttcca ggtgaccccg
     4381 gggatctcgg ccggcggttc ggcgtcgtcg aacagcgcgg ggttggcgaa ctcgaccgtg
     4441 ccgctgccgc tgccggcgaa ccggatgaac ttcagccgga tcagcaggtg ctcgccgctg
     4501 acgtcgacgc ccgcggcggt gctgctgaaa cgcgccagcg agatgttggt cccgcccggg
     4561 atcgggtcgg tgatgatctc gaccggcacg ccccccgacc cgagcaccga gcccgacgtg
     4621 tccggtgcgc gcaaggcgac gagattggtc gggaacagca ggtcgaacga gacgccgaac
     4681 aacggttgcc ccacgcccga gatcgtcacg tcgacccaca gctcgtcgca atcgcccgac
     4741 agcccgtcga ccgccacgac cgtgcccggg accgggtcga cggcgttcgc caactcgacg
     4801 cacggagtgg tcacgatgcc gccgcccccg ccgccgctgt cgtcgccgcc gcagccggcg
     4861 gacaaaccga cgagcagcag cccgagcgcc ggcagcacgg cccgtcgggg cttccgtgcg
     4921 gtgatcggtc gggctcgcat catcgtcgtc tctccgtcgg tctcgtcgtc gtggtcaatt
     4981 gcagacgtag ctgcagctat cggtggtgaa gccgaacacg ccgtcgctcg cgaggatcgc
     5041 cagatcctgc ccgtcgatcg tgccgtcggc gttgtagtcg acggcgatca tccattgggg
     5101 cgaggcagga ccgaccgcgc gaccgaagcc gcggccgatg taggccagct ccacgccgtc
     5161 gattcggccg tcgccgctgg cgtcgaaacc ctgacaggtc tccccgccgg cctggtacca
     5221 gagtacagcg aggcgctggc cggagacgat gtccggcata ccgtcaccgt cgaggtccgc
     5281 cacgtcggac gagctggcga agtcgatctc cgtcgcgacg aggtcgacgg tgaagatcgc
     5341 cgcgccgtcg ttgtcgaacc agacgatcac atcgtcgccc gggatggtgg cgacgatgtc
     5401 cggatctccg tccggcaccg cgtcgaggtc ggcggcgaac acctcgctga tcccgtcggc
     5461 gctcgtcgcg aggacacgcg tggcgaacgc cggtggcgac ggtggcggct gttccggatc
     5521 gtcgtcgagg ttgctcgaga gcagcgccac gacctccgat tccgacgcgc tcaccacgat
     5581 gtcgatgtcg ccgtcgccgt cgaaatcggc ggtgtcgatc gacgtcggcc cgagcagacc
     5641 gacgtcgaac tcccgcgtca gcgggaaggc gggaccgtcg gcgtcgggca cgttctcgag
     5701 ccagacgacg cggtcgatgc cgaacagcgc ggccagcacg tcgacgtcgc cgtcctcgtc
     5761 gaggtcgacc gcgtgcaccg agcgcgcgcc gcccgcgtcg tcggtgatca actgcggcgg
     5821 ggagaagccg ccaccgccgt cgttctcgta ccactggatc cggccgaacg gcggcgtcgg
     5881 ttcgtcctcc tcgacctcgg gtgcctcgcc ggcctcggcc gagatcaggt cgaggtcgcc
     5941 gtcgccgtcg aggtcgaccg cgacgatctt ctgcaggttg tcgagaccgg cggcgacatc
     6001 gacgctcgcc tcggtaaaaa cgggaaccgg tgccccgcgc acggcgccgg tgatcaggcc
     6061 gttgtcgaac gcgcggatcc ggccatcgtc ggccgaggcg acgaccagct cgaggtcggt
     6121 gtcgccgtcg agatcgaccg cgacgacgtc gatcgcgccg agcacatcct cggccacgat
     6181 ctgctcggtg aagctcggcg ggtcgccgcc gtcgttgcgg taccaggcga gacgatcgga
     6241 tcggccggcg gtgaccacgt cgacccgccc gtcgccgtcg aggtcggcgc cgatcgctcg
     6301 cacggcgccg tccgcgctgc gcgagatcgt cgagccctcg tcgaaaccgg atccgttgtt
     6361 ccggtaccag gcgatgcgat cgcgggtggc ggggaagccc gccaccgacg agaccgacac
     6421 gatgtcgacc gccccgtcgc cgtcgaggtc ggccgcgccg acgtcgtcgg cgctgagcac
     6481 gtcggttgag accgggatct cgttgaagcc gggcggctgg ccgccgacgt actcgtacca
     6541 cgcgatcgtg tcgtcgccgt agctggccga cagcagatcg ggtttctgat ccaggttgac
     6601 gtcggcgacg cgaaccacgc ggaccagctg cgcgtcgttc gtcacgtcga actcgctgaa
     6661 cgccggcggt tgtccgccgt cgttccggta ccagatgatc cggttctcgt tgagcgcggc
     6721 ggcagcgacg tcgagccggc cgtcgtcgtc gaggtcggcg acggtgaccg agctcgcgcc
     6781 gatggccgac gacaccggct ggaccacgca ggtctcgggc tggtccatcg gctcggcggg
     6841 cggcgggcag tctgcggcga tgtcgcagcg aacgtcggtc gatacgctgc aacgaccgtc
     6901 gaggccggcc agcacgatct tgtcgaacgc cgggatcgtc cccggtgcgc cggcggtctg
     6961 gcgatagagg accacctccc catcgaggct cgaggcggcg accacgtcgg ttcgcgtgtc
     7021 gccgtcgacg tcggccgccc acactgccgc ggcgcccagc gcccgctcgg tgatgacgaa
     7081 ccgcgtgaac tcgatcggag tggtcccgct gttgcgatac caagcgatcg tgtcgtcgag
     7141 gaacgacgcc gtcaacacgt cgacgtggcc gtcgccgtcg acgtctgccg cgtgaacccc
     7201 gagcgccagc tccgtctcga gcgtgaccgt gcgcttgctg aacaccggcg gctggctgcc
     7261 ggccgcctgc tcgaaccaga aaacgccccg ctcgcgcgag gcggcgatca ggtcgaggtc
     7321 ctggtcgccg tcgaggtcga ccgcgaccac gtccgaggcg ctgcccagat cgtccgcgat
     7381 caggtgcggc aggaaccgag gcggggtcgg ctgatcgctg cgattctcgt accagaacag
     7441 cttcccgtca ccgaacgacg cggtgacgag gtccgggcgc ccgtcgcggt cgatgtcggc
     7501 cacgaccagc cgttcgacgc cgttggtccc cccgtcgacc accgggcgct cgacgaaacc
     7561 gagacactct ccctgggccg cgatcggaac cgccgccgcc ccggcgatca cgccgagcgc
     7621 gagcatcgtg agttcgacta cccgtttcat ccggcgtcct tcgcagcggt cggcgagatg
     7681 gaagctcggg ccgagagtgg cttgtgtacc accgatccgc cccgttgtcg atgatctcgc
     7741 gcgatcgccg cggaccggta accgcctgcg gcggccgggt ttggccgccg cgttcctcgg
     7801 atgataagat cgttggttcg cgaaccggag ggaaatcgat gatcgaggca ccggcatgct
     7861 gaggcaagcg ttccatccga tccgggtgac ggtcctcgcg gtcgtcgcct tcgcgccgca
     7921 cgtggcggtc gccgaacggc tcgacgatcc cgccgcgctg accgagcaag cgccggaccg
     7981 gttctccgtc gagttcgaaa cgacgaaggg caagttcgtc gtcgacgtga cgcgcgcctg
     8041 ggcgccgctc ggcgcggatc gtttctacaa cctggtcaag cacggtttct acgacgacgt
     8101 acgcttcttc cgcgtcgtgc ccgacttcgt cgtgcagttc ggtctgaacg gcgacccggc
     8161 gatcggcaag ccctggcaga gtgcccgcat caaggacgac cccgtcacgc ggagcaacga
     8221 ggccggcacg ctgacgttcg ccatggccgg accgaactcg cgcacgacgc aggtgttcat
     8281 caatctgaag tccaacaagt cgctggacca atcgggcttc tcgccgttcg gcgaggtttc
     8341 cgcgggaatg gacgtcgtca ggaagctgta cgcgggctac ggcgaccaac cgccgcgcgg
     8401 acgcggaccg aaccaatcgc agatcgcgat ggaaggcaac gagtacctcg acaggagctt
     8461 ccccaagctc gacgcgatcg tcgacgcgac gttgatcgag aacgagaccg cggacgaggc
     8521 ggccggcggt gactgaccgg gacccggggg gcgcgcgcga cgtgatcgac ttccggatcg
     8581 acgtcgagtg ctacgcgggc catcgcggtg aggaaacgcc gcggcgcttc gccgtcggcc
     8641 gcgcgacgat cgaggtggcc gacgtcgtcg atcgctggct cgcgcccgag catcgctact
     8701 tcaaggtgcg gggagacgac ggcggcctgt acatcttgcg ccacgatccc gtcgcgggta
     8761 gctggagcct gacgatgttc gaaagcgccg ccctgcgcga cgcgtagcct cgcttcaacc
     8821 gtcgctcgat cgggtctagt cggcgtccgg ccgacccgcg atccgatcga tccgctcgat
     8881 ctgctcgcgc gcgtgcgcgc cggagcgcga cgggtcgacc tcgatcgcgc gggcgaactc
     8941 ggcgcgcgcc tcggccaggc ggccctccat cgccagacgg tgaccgagac cgatgtggat
     9001 cccgccgtgc cgtgcgtcga gcttccgggc ctcggccagc gcaccgtcga actcggccaa
     9061 gtcccgccgg ttgagcgcat agcgggccag gatgacccaa ccgtcggcgc tcgggctgag
     9121 cgccagcgcg cgacgcaccg cctgctcggc tgcgtcgtga cgaccggcgc gatcgtagat
     9181 ccggagcgcg gtgtaccacg ccttgccgct cgccgggtcg gcgtcgagca cctcgccgat
     9241 cagctcggcc gccccgtcgt ggtcgccggc cagcgacagc ttgccggcgc ggtcgatctt
     9301 gtcccacaac ggcatcatcc gcttcgggtc ctgcgcgccg atcgcggccc cgctcggcgg
     9361 cgcccgcagg tagcccaacg aagcgagccg ttcgagttcc tcctcgtcga gcgcgcgctg
     9421 ccggtcgacc gccgccgcgt cgtccggcca gcccgcgagc cgcgcgtcga gtcgcgcctc
     9481 gagcgcgccg gccgccggcg aatcgtcgta gagatcgacc gtctcgcccg ggtcgtccgt
     9541 cacgcggtag tactccggct ccggcgcacg gatcaacttg tccccgagcc gacgcagacc
     9601 gcgaagcggc gaccagccgt agtccagcaa cggcacgagc gattcgacgt agatcgcgcg
     9661 gtcgtcgtcg accgcggctg cgaacaggtc gcgcccgtcg acctccgcct gcccggcggc
     9721 gacgccgagc agaccgagca cggtcgggaa gacgtcggtg ccacccgcga cgcggtcggc
     9781 gatgtcgacg ccgcgggcaa agagccgggg gtgcgacacg atcaacggca cccggaccgt
     9841 actgtcgtag atcaacacgc cgtgcgtcag ctcgtcgtgg tcgccgagac cctcgccgtg
     9901 atcggcggtc agtacgacga gcgtgcgttc gagcaggtcg gcctgcgcga ggaattcgag
     9961 cagccggccg atctgcgcgt cgcagaaggc gatctctctg tcgtacggcc ggccggggag
    10021 catcgccgcg tactcggtgg gaggctcgta cggcatgtgc gggtcgaaca ggtggaccca
    10081 ggccaggaac ggccggcgcg ggtcgaccgc gatcgtccgc tcgagccagc cgatcgccgc
    10141 gtcgacgact tcgttcgccg gtcgctcgtt gtagcggccc atcgacggcg tcgccgcgag
    10201 gtcgtgtacg gcgtcgtcat agtgatcgaa gccctgggcc aacccgtaac gggcgtcgag
    10261 cacaaacgcg ccgacgaacg cgccggtggc gtaaccctcc cgcgcgagcg cctcggccag
    10321 cgtccacgcc tcgtcgccca agcgataggt gccgttgtgc ctgaccccgt gtcccggcac
    10381 gtcgaggccg gtcatgatcg tcgcgtggga cggcagcgta acgggtgcgc cacaaatcgc
    10441 ttggtcgaag cgggcgccgc gcgcggcgag gccgtcgatc accggcgtcc gcgcggactc
    10501 gccgccgtag cagccga
//
LOCUS       DASPGN010000381         3330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1472800, whole genome shotgun sequence.
ACCESSION   DASPGN010000381 DASPGN010000000
VERSION     DASPGN010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472800"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VD788_10985"
     CDS             <1..990
                     /locus_tag="VD788_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HXV76827.1"
                     /translation="GSIKMLPSPIAERAASLVPGERRPTLSFLVDFAQDGEPIGSRIV
                     RGIVRSRARLDYDEADRWLAETDGRWGAVLAALDRWARARELRRIEAGAIVIRAAEVD
                     VWVDPDGGVRLERIEPDSASRRIVGEAMVLAGELAAARCVAAGIPAIYRRQPPSDRLP
                     ALPAGAATDPVTVRRVRRALSRSDSATRPGRHHGLGIDAYVQVTSPIRRFQDLTIGRQ
                     LAAHLAGVLPPYDAEAIQRVAATTERAEIEARRAERAAVDYWLLRYLEQSVGRTVEAI
                     VVETAPRPVVLLSETLREQPMPSLARVEPGERLLLRVERVNPRAGLLALRRVD"
     gene            1069..2706
                     /locus_tag="VD788_10990"
     CDS             1069..2706
                     /locus_tag="VD788_10990"
                     /inference="COORDINATES: protein motif:HMM:NF045305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HXV76828.1"
                     /translation="MILPARSQPDLPLDRLIRHEAPGADATPLDVLFVGAGPAGLAGA
                     IWLAKLIREDGGLGEIEIGVLEKAESLGEHSLSGAVVNPRPFRMLFPDLDDDALPFRG
                     PVRQDRVYLLTEGAARRIPTPREMHNEGNFVASICEIVRFLGERAEGLGVNLFTGFPA
                     ESLLVDGDAVVGVRTAPAGLDRDGKPGGGYIPPNDLTARVTVLSDGTRSALAQGWMKW
                     QDVGSANPQIYALGVKELWEVKRAPEAVIHTMGWPLPADAFGGSWCYPMGPNLVSLGL
                     VVGLDYRESGFDPHESMQKLKTHPLFREMLDGGELVEWGAKTIPEGGYHSLPDRLSGD
                     GLILTGDAAGLVNVATLKGIHYAMQSGIFAAHTIFDALRKADSSSSALASYDRRLRDS
                     YVTHDLRRTRNMRLAFKSGFWLGSAKAALMSLTGGRFPGGRIAVDSDADEERVARRAG
                     EFRPDGKLTFAKLDAVYRSGNSTRDDIPSHLIVGEAVDGEIAEFYRHLCPAGVYERDG
                     DRLVVNPPNCVDCKATDVVGPRWMPREGGSGPKYKRM"
     gene            2818..>3330
                     /locus_tag="VD788_10995"
     CDS             2818..>3330
                     /locus_tag="VD788_10995"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HXV76829.1"
                     /translation="MQVERIRPTLFSRLYLPSILAGLRVTFRHIFRRKDTLQYPEEQR
                     VFGERYRGVPALVKDQHGRTKCVACYMCQWVCPPLAITIEAAEIPASDHERAQIEKEP
                     SVFEINMLRCIYCGLCEEACPEEAIFMSKTYAVTGLSRELMIFDKEKLLEIGGIREDA
                     TRKWAPENKGP"
BASE COUNT          500 a         1134 c         1200 g          496 t
ORIGIN      
        1 gggagcatca agatgctccc gtcgccgatc gccgagcggg cggcgagcct ggtcccgggc
       61 gaacgccggc cgacgctcag cttcctcgtc gacttcgcgc aggacggcga gccgatcggt
      121 tcgcggatcg ttcgcggcat cgtccgttcg cgcgctcggc tcgactacga cgaggccgac
      181 cgctggctgg cggaaacgga cgggaggtgg ggagcggtgc tggccgcgct cgaccgttgg
      241 gcccgcgcgc gcgaactgcg ccggatcgag gcgggtgcga tcgtgatccg cgccgccgag
      301 gtcgacgtgt gggtcgatcc cgacggcggg gtgcggctcg agcggatcga gcccgactcg
      361 gcctcgcgcc gcatcgtcgg cgaggcgatg gtgctcgccg gcgagctcgc ggccgcacga
      421 tgcgtcgccg ccgggatccc ggcgatctat cggcgccagc cgccgtcgga ccggttgccg
      481 gcgctcccgg ccggcgcggc gacggatccg gtcaccgttc ggcgcgtacg ccgggcgctg
      541 agccgcagcg acagtgcgac gcgtcccggg cgccaccacg gtctggggat cgacgcctac
      601 gtgcaggtga cctcgccgat ccgccgcttc caggacctca cgatcggccg gcagctggcg
      661 gcccatttgg ccggcgtcct gccgccctac gacgccgagg cgatccagcg cgtggccgcg
      721 acgaccgaga gggccgagat cgaggcgcgc cgggccgagc gcgccgccgt cgattactgg
      781 ttgctgcgct acctcgagca gagcgtcggc cggaccgtcg aggcgatcgt cgtcgagacc
      841 gcgccgcgcc cggtcgtgct gctgagcgag acgctgcgcg agcagccgat gccgtcgctc
      901 gcgcgggtcg agccgggcga gcgtctcttg ctgcgtgtcg agcgggtcaa cccgcgggcc
      961 gggctgctcg cgctgcggcg cgtcgactga tccggtggtc ggtttcaaag cgaggcgaaa
     1021 cctggcatga tgagcggtcc cgtcgatccg cgaccgagga cgcgaacgat gatcctaccc
     1081 gcacgctccc aacccgatct gccgctcgat cgcctgatcc ggcacgaggc gccgggggcg
     1141 gacgccacgc cgctcgacgt gctgttcgtc ggcgccggcc cggccggtct ggccggagcg
     1201 atctggctgg cgaagctgat ccgcgaggac ggcgggctgg gggagatcga gatcggcgtg
     1261 ctcgagaagg ccgagagcct cggcgagcac agtctgtccg gcgcggtggt caacccgcgg
     1321 ccgttccgca tgttgttccc cgatctcgac gacgacgcgc tgccgtttcg cgggccggtc
     1381 cgacaggatc gggtctacct gttgaccgag ggtgcggcgc gccggatccc gacgccgcgc
     1441 gagatgcaca acgaggggaa cttcgtcgcg tcgatctgcg agatcgtgcg ctttctcggc
     1501 gagcgggccg aggggctcgg cgtcaacctg ttcaccggct tccccgccga gtcgctgctc
     1561 gtcgacggcg acgcggtcgt cggcgtgcgt accgcgcccg ccggtctcga tcgcgacggg
     1621 aagccgggtg gcggctacat accgcccaac gacctgacgg cgcgcgtgac ggtgctcagc
     1681 gacggcacgc gcagcgcact ggcgcagggc tggatgaagt ggcaagacgt cggctcggcc
     1741 aacccgcaga tctacgccct cggcgtcaag gagctgtggg aggtgaagcg tgcgcccgag
     1801 gcggtgatcc acacgatggg ctggccgctg ccggccgacg cgttcggcgg cagctggtgc
     1861 tacccgatgg gtccgaacct cgtctcgctc gggctggtgg tagggctcga ttaccgggag
     1921 agtggcttcg acccccacga gtcgatgcag aagctcaaga cccacccgct gttccgcgag
     1981 atgctcgacg ggggcgagct ggtcgaatgg ggggcgaaga cgattcccga aggcgggtac
     2041 cactcgcttc ccgatcggtt gagcggggac ggcctgatcc tgaccggtga cgccgcgggc
     2101 ctggtcaacg tcgccacgct caagggtatc cactacgcga tgcagtcggg catcttcgcc
     2161 gcgcacacga tcttcgacgc gttgcggaag gccgacagct cgtccagcgc gctcgcgtcc
     2221 tacgaccggc gattgcgcga cagctacgtc acccacgatc tccgccgcac gcgcaacatg
     2281 cggttggcgt tcaagtccgg attctggttg gggagcgcca aggccgcgct gatgagcctc
     2341 accggcgggc ggttcccggg aggacggatc gccgtcgatt ccgacgccga cgaggagcgg
     2401 gtcgcgcggc gagcgggtga attccgtccc gacggcaagc tgaccttcgc caagctcgac
     2461 gcggtctacc gcagcggcaa ctcgacgcgc gacgacattc cgtcgcacct gatcgtcggc
     2521 gaggcggtcg acggcgagat cgccgagttc taccgacacc tctgtcccgc cggagtctac
     2581 gagcgggacg gcgaccggct cgtcgtcaac ccgccgaact gcgtcgactg caaggcgacc
     2641 gacgtggtcg gtccgcgctg gatgccgcgc gagggcggca gcgggccgaa gtacaagcgg
     2701 atgtgaggcg tgcgccatcc gcggcaagtc tcttggattc aatcgtttgc ctgcatgatc
     2761 cacactgtgt taccatcctc ggcccgcggt cgtcgaccac ccgccgccga ggtcccgatg
     2821 caagtcgagc gcatccgacc caccctgttc agccggctct acctgccgag catcctcgcc
     2881 gggctgcgcg tgaccttccg tcacatcttc cgccggaagg acacgctgca atacccggag
     2941 gagcagcgcg tgttcggcga gcggtaccgc ggcgtgcccg cgctcgtgaa ggatcagcac
     3001 ggtcggacca agtgcgtcgc gtgctacatg tgccagtggg tctgcccgcc gctcgcgatc
     3061 acgatcgagg ccgccgagat tccggcaagc gatcacgagc gcgcgcagat cgaaaaagag
     3121 cccagcgtgt tcgagatcaa catgctgcgc tgcatctact gcggcctgtg cgaggaagcc
     3181 tgtccggaag aggcgatctt catgtcgaag acctacgccg tcaccgggct gtcgcgcgag
     3241 ctgatgatct tcgacaagga gaaactgctc gagatcggcg ggatccgcga ggacgcgacc
     3301 cgcaagtggg ctcccgagaa caagggaccg
//
LOCUS       DASPGN010000382         3435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_304251, whole genome shotgun sequence.
ACCESSION   DASPGN010000382 DASPGN010000000
VERSION     DASPGN010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304251"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1469)
                     /gene="cls"
                     /locus_tag="VD788_11000"
     CDS             complement(6..1469)
                     /gene="cls"
                     /locus_tag="VD788_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HXV76830.1"
                     /translation="MDRRRPCRLLSASLNTALAAAAFLVALVAAGHALLNKRDPRAAL
                     GWITVSLLFPLVGPVLYFVFGINRISTRAQKMGHRWSRLSQPHHVHPSADAEEREERD
                     IPAKFTALARVSRAVTGLPLARRNLVVPFENGEGAYTPMVDAIDEATSTVYLETYIFE
                     TDGTGRRFIDALARAARRGLDVRVLIDGIGALYSRPRASSLLRRHGIRVARFLPPRLL
                     PPSLNVNLRNHRKILVIDGHRSFTGGMNIGGRHMVSDPANRSPTVDLHFRVDGPVVRQ
                     LEDTFLADWGFATGDPPPERRNPLPEPAGDALCRVILDGPDEDLGKLATVINGAISAA
                     RSRVAILSPYFLPPREMTSALQAAALRGVDVMVVLPEKSNLRFVDWATRNMLWELLER
                     GVDVFYQPPPFAHTKLLLVDDHYVQVGSANMDPRSLRLNFEFAVEVYDSRMGRVAAEY
                     VDRIVARSRRVTLDEVDSRSLPVRLRDSVAWLFQPYL"
     gene            complement(1439..1603)
                     /locus_tag="VD788_11005"
     CDS             complement(1439..1603)
                     /locus_tag="VD788_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76831.1"
                     /translation="MSPLLPDADAGTEIDVDADPLFIDSDERTTLTNPDGFNGVHDTD
                     VWIVGARVGF"
     gene            1811..2869
                     /locus_tag="VD788_11010"
     CDS             1811..2869
                     /locus_tag="VD788_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HXV76832.1"
                     /translation="MSASEFGDKLRREREDRRTSIEEIAERSKIDLRYLEALEGGDFD
                     ALPGPAIGKYYVRVYAEKLGFEPAAWIADYEHAQNLRRLPAIKARRRARREPRRVEAA
                     IARWRAETAARTDPDGSADPPPVEAEFAGVRPQPPDAPVDEPQPIVAAADDCATATSA
                     SAGTAEPARRSVTPLATAATLAGVLSIAAWFWWSVQPDSAVSAPGARSAAPALAARVE
                     ARPVGSPVPTPRPSVARPAAVDSPRPESRLSVTESGLGRAVERNRLVGAATEFPEGSA
                     AVFLTRVVGGARGDRLRHVWIRDGRTVQTIELPIGGSHWRTHSRKTLYGLGPWTVEAW
                     DARGRVLASVSFRCVAGR"
     gene            complement(2885..>3435)
                     /locus_tag="VD788_11015"
     CDS             complement(2885..>3435)
                     /locus_tag="VD788_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76833.1"
                     /translation="SSRRSAGRDAVERSASVGASLVWLLAAHLATVVAFADAAPCADA
                     KSRQFDFWIGEWEVTANGTVAGHNSIRPILDGCVLQETWSGVKGSAGSSFNFFDPQRG
                     KWRQLWVWRAGTTLELEGEFADGRMTLEGTSVGTDGRAVRNRVTWYDNDDGTVRQHWQ
                     IVEPGSEEWKTVFDGFYRKRSD"
BASE COUNT          536 a         1180 c         1191 g          528 t
ORIGIN      
        1 cggcgctaca agtacggctg gaacaaccag gcgaccgaat cccgcagccg gaccggcagg
       61 ctgcgactgt cgacctcgtc gagcgtcacc cgacgcgaac gggcgacgat ccgatcgacg
      121 tactcggccg ccacacgacc catccgcgaa tcgtagacct cgactgcgaa ttcgaagttc
      181 agcctcaggc tgcgtgggtc catgttggcc gagccgacct ggacgtagtg gtcgtcgacg
      241 agcaacagct tggtgtgggc gaacggcggc ggctggtaga agacgtccac ccctcgttcg
      301 agcagctccc acagcatgtt gcgcgtggcc cagtcgacga accgcagatt gctcttctcg
      361 ggcagcacga ccatgacgtc gacgccgcga agcgccgcag cctgcaacgc ggaggtcatc
      421 tcacgcggcg gcaggaagta cgggctgagg atcgcgacgc ggctcctggc cgccgagatc
      481 gctccgttga tcaccgtggc gagcttgccg aggtcctcgt cgggtccgtc gagaatcacg
      541 cggcacaacg cgtcgcctgc cggctcggga agcgggttgc gtctctccgg cggcgggtcg
      601 ccggtggcga agccccagtc ggcgagaaag gtatcctcga gctgccgcac caccgggccg
      661 tcgacgcgaa agtggagatc gaccgtcggc gaacggttgg ccggatccga gaccatgtgg
      721 cgcccgccga tgttcattcc tccggtaaac gaacgatggc cgtcgatcac caggatcttg
      781 cggtggttgc gcaggttcac gttcagcgac ggcggtagga gccgcggcgg gagaaaacga
      841 gcgacccgaa tcccgtgcct tcgcagcagc gagcttgcac ggggacggga atagagcgcg
      901 ccgatcccgt cgatcaacac ccgcacgtcg agcccccggc gcgcggcgcg cgccaacgca
      961 tcgatgaagc gtcggccggt gccgtcggtc tcgaagatgt aggtctcgag gtagaccgtc
     1021 gaggtcgcct cgtcgatcgc gtcgaccatc ggcgtgtagg caccctcgcc gttctcgaac
     1081 ggcacgacga ggttgcggcg tgcgagcggc aggccggtga ccgcgcgcga gacgcgggcg
     1141 agcgcggtga acttggccgg gatatcgcgc tcttcccgtt cctccgcgtc ggccgacgga
     1201 tggacgtggt gcggttggct cagacgcgac cagcggtgcc ccatcttctg ggcgcgcgtg
     1261 ctgatgcggt tgatcccgaa cacgaagtac agcaccggcc cgaccagcgg gaacagcagg
     1321 ctgaccgtga tccagcccaa ggccgcgcgc gggtcgcgct tgttgagtag cgcgtgcccg
     1381 gccgcgacca gggcgacgag gaacgcggct gccgcgagcg cggtattgag cgacgcgctc
     1441 agaagccgac acgggcgccg acgatccaca cgtcggtgtc atggacgccg ttgaatccgt
     1501 ccgggttggt cagcgtcgtc cgctcgtcgg aatcgatgaa caacggatcg gcgtcgacgt
     1561 cgatctccgt gcccgcatcg gcgtccggca gcaacgggct catcgtcgcc tctgcgcggt
     1621 agtccgtctc gttcgttgcc gccgctcccc gagaagcccg catggtcgac cccctttgat
     1681 cggcccggcc catccctcgt tcattcagct aggccggggg cgcggttgtc aagatccgat
     1741 ccgctcgacc tgcggttgtc tcacggaacc cgcgggaggg tcaaccaggc aggcggtcta
     1801 tactcttccc gtgtccgcgt ccgaattcgg tgacaagctc cgtcgagagc gggaagaccg
     1861 tcggacctcg atcgaggaga tcgccgagcg gtcgaagatc gatctgcgct acctcgaggc
     1921 gctcgagggc ggcgacttcg acgcgcttcc cggacccgcg atcggcaagt actacgtccg
     1981 ggtctacgcc gagaagttgg ggttcgagcc cgcggcgtgg atcgccgact acgagcacgc
     2041 gcagaacctg aggcgtctgc cggcgatcaa ggcacgtcgt cgcgcgcggc gggagccgag
     2101 gcgcgtcgag gccgcgatcg cgcggtggcg cgccgagacg gccgcgagga ccgacccgga
     2161 cgggagcgcg gatccgccgc cggtggaggc agaattcgcg ggcgttcgac cgcagccccc
     2221 cgacgcgccg gtggacgagc cgcaaccgat cgtcgcggcc gcggacgact gcgcgaccgc
     2281 gacgagcgcc tccgcgggga cagccgagcc tgcgcgccgc tcggtgactc cgctcgccac
     2341 cgcggcgacg ctcgccgggg tgctttcgat cgcggcctgg ttctggtggt cggtccaacc
     2401 cgactcggcc gtcagcgcac ccggtgcgag atcggcggcc ccggcgctgg ccgcccgcgt
     2461 cgaggctcgg cccgtcggct cccccgtacc gacgccgaga ccatccgttg cgcgtccggc
     2521 ggcggtcgac tctccgcgcc cggagagcag gctgtcggtg acggaatccg gtctcggtcg
     2581 cgcggtcgag cgcaaccgcc tggtcggggc cgcgaccgag tttccggagg ggagcgccgc
     2641 cgtcttcctc acgcgcgtcg tcggcggtgc gcgtggggat cgcctgcgcc acgtttggat
     2701 ccgcgacggc cggacggtcc agacgatcga gcttcccatc ggcggctccc actggcgaac
     2761 ccacagccgc aagacgctct acgggctcgg tccgtggacg gtcgaagcgt gggacgctcg
     2821 agggcgcgtg ctggccagcg tgtcgtttcg ctgcgtcgcc gggcggtagc ctatgcgtcc
     2881 gtattcaatc ggaacgcttc cgatagaatc cgtcgaagac cgtcttccat tcctcggagc
     2941 cgggttcgac gatctgccag tgctggcgga ccgtgccgtc gtcgttgtcg taccaggtga
     3001 cgcggttgcg caccgcacgc ccgtccgtcc ccaccgaagt gccctcgagc gtcatcctgc
     3061 cgtcggcgaa ctcgccctcg agctcgagcg tggtaccggc acgccagacc cagagctggc
     3121 gccacttgcc ccgctgcggg tcgaagaagt tgaaactgga gccggcgctc cctttcacgc
     3181 cgctccacgt ctcctgcagc acgcagccgt cgaggatcgg acggatcgaa ttatgtccgg
     3241 cgacggttcc gttcgcggtc acttcccatt cgccgatcca aaaatcgaat tggcgggatt
     3301 tcgcgtcggc gcacggcgcc gcatccgcga aggcgacgac ggtcgccagg tgtgccgcca
     3361 gcagccagac gagcgatgcg ccaaccgagg ccgatcgttc cactgcgtcc ctcccggcag
     3421 atcgtcggga gctta
//
LOCUS       DASPGN010000383         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1122515, whole genome shotgun sequence.
ACCESSION   DASPGN010000383 DASPGN010000000
VERSION     DASPGN010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122515"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..1222
                     /locus_tag="VD788_11020"
     CDS             194..1222
                     /locus_tag="VD788_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXV76834.1"
                     /translation="MPALQMTGLARIPLNLLAGAEQYELDRDELSAAAGLTPSELADP
                     DTRVPLSKIWALWRVLIERTDDPALGLRLGMRTRVRELGLVGYAVRHSRTLREAIGRV
                     ARYSRIVNEALVLHLIENENHGKLIVERAPALDELIHPTDMRLASALAVAREITGAHI
                     VPREVGVPHARPADVSAHERFFSCAIAFDQPESVLVFRCEDLDRAAIWADATLIGYLD
                     RLADEMLASLDETVTFRERVRRAIWFDLSGGKPSVRQVARQLGVSPRTLQRRLEENDT
                     SFAIELDRLRHEMAVRLLQDRSLAVYEVAFLLGYSEPSTFYRAFRRWERRSPQEYRRT
                     VGDPADRR"
     gene            complement(1219..1764)
                     /locus_tag="VD788_11025"
     CDS             complement(1219..1764)
                     /locus_tag="VD788_11025"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXV76835.1"
                     /translation="MLLATRRRPTATLLLLTLLAWATVVPALADGNTTFSGRVFGTSA
                     SEPEAGVVVALVEPTTERVYSSEPSAADGTFRVDGAPAGTYRLVAETPRGAYLADAPV
                     EVTPGGNRPVALALGGTDPAYQTTQDPTGQAQGDLPAWAKWTIVGGIAVLGLLAIDSL
                     TDDESEEAPVSPSILGPEIDR"
     gene            complement(1838..2422)
                     /locus_tag="VD788_11030"
     CDS             complement(1838..2422)
                     /locus_tag="VD788_11030"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXV76836.1"
                     /translation="MRRSITVIPLGLALLVQAAGALAEETVEPAPAAGQGQLVGQVST
                     PSGTAVSGATVVAYHLSSESVFRSADTDAKGRFKIAALPYGYFDIAVQTGDGLYVADQ
                     VVNVPPAGKASLTMTLYAGAADQEAPRGFAGIDQAPSGVARVESKASAGQFWKSAKGV
                     AILGGIGGAALLAIALSSDEDEDEISVSPSTLAD"
     gene            2521..2856
                     /locus_tag="VD788_11035"
     CDS             2521..2856
                     /locus_tag="VD788_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76837.1"
                     /translation="MPPLTPRTLDIEPDPRETAPVEVRTRPPREHRAARRSPRHSHTR
                     VRMLYFGLSSVWGFLAGSVAVLVAFDAVGHPITLRGELGTALSIAAVLAVVGGVIVSM
                     AYRELAGRR"
     gene            complement(2868..>3170)
                     /locus_tag="VD788_11040"
     CDS             complement(2868..>3170)
                     /locus_tag="VD788_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76838.1"
                     /translation="HYVGNRFLTLVSNAVTNLNLTDMETCYKMFRGDLLRGLDLREER
                     FGFEPEVTAKISRLDVRIYEVGISYWGRTYAEGKKINWRDGVEALACILRYGLLRG"
BASE COUNT          456 a         1110 c         1100 g          504 t
ORIGIN      
        1 gaccacccgg tgctgcccga cgaactcgtc gagatcgcgc gggcgcatcc gatcggccag
       61 cggcacccgc gccgagccgg acggcggcgg tggcgatggg tcgaacagcg acatcgaccg
      121 attagtgtag aggagtcggc cgccgccggg cacgagtgtt agaattccgg gcggttcgag
      181 ctgtgagggt cgagtgcccg cactccaaat gaccggtctg gcccgcatcc cgctcaacct
      241 gctggcgggt gccgagcaat acgagctgga ccgtgacgaa ttgtcggcgg cggccgggct
      301 gacgccgagc gagctggccg accccgacac ccgcgtgccg ctcagcaaga tctgggcgtt
      361 gtggcgggtg ttgatcgagc gcaccgacga tccggcgctg gggctgcgtc tcggtatgcg
      421 gacccgggtg cgcgagctcg gtctcgtcgg ctacgccgtg cgccacagcc gtacgctgcg
      481 cgaggcgatc ggccgcgtgg cgcgctacag ccggatcgtc aacgaggcgc tggtgctgca
      541 cctgatcgag aacgagaacc acgggaagct gatcgtcgaa cgggcccccg cgctcgacga
      601 gctgatccac ccgaccgaca tgcggctggc gtccgcgctc gccgtcgcac gcgagatcac
      661 cggcgcgcac atcgtacccc gcgaagtagg cgtgcctcac gcgcggccgg ccgacgtcag
      721 cgcccacgag cggttcttct cgtgcgcgat cgcgttcgac cagcccgaat cggtgctggt
      781 gttccgctgc gaggatctcg atcgagcggc gatctgggcc gacgccacgt tgatcggcta
      841 cctcgaccgg ctggccgacg agatgctcgc gtcgctcgac gagaccgtca cttttcgcga
      901 gcgcgtgcgt cgcgccatct ggttcgactt gagcggcgga aagcccagcg tgcggcaggt
      961 cgcgaggcag ctcggcgtca gtccgcgcac gctccagcgc cggctggagg agaacgacac
     1021 ctcgttcgcc atcgagctcg atcggttgcg acacgagatg gcggtgcggc tgctgcagga
     1081 ccgctcgctg gcggtttacg aggtggcgtt cctgctcggt tactccgagc cgagcacgtt
     1141 ctaccgcgcc ttccgccggt gggagcgccg ctcgccccag gagtaccggc gcacggtcgg
     1201 tgaccccgcc gatcgacgct agcgatcgat ctccgggccg agaatcgatg ggctgaccgg
     1261 ggcctcctcg ctttcgtcgt cggtgagcga gtcgatcgcc agcaggccga gcaccgcgat
     1321 gccaccgacg atcgtccact tggcccatgc cggcaggtcg ccttgggcct ggccggtggg
     1381 gtcctgcgtg gtctggtagg ccggatcggt gccgccgagg gccagggcca ccggccggtt
     1441 gcctcccggc gtcacctcga ccggcgcgtc cgcgaggtac gccccgcgcg gggtctccgc
     1501 caccaggcgg taggtgcccg ccggggcgcc gtcgacgcga aaggtcccgt ccgctgccga
     1561 tggctcggag ctgtagacgc gttccgtcgt cggctcgacg agcgcgacga ccacaccggc
     1621 ttcgggttcg gaggccgacg ttccgaagac cctcccgctg aaggtcgtgt tcccgtcggc
     1681 gagcgcggga acgaccgtgg cccacgcgag cagcgtcagg agcaagagcg tggccgtcgg
     1741 acggcgccgc gttgccagca gcatcgagtt tcctcccgcc acgcaccgtg tggccccgcc
     1801 attctccggc cggtgtcgat tcctgtcaag cgaccggtca gtcggcgagc gtggaggggc
     1861 tgaccgagat ttcgtcctcg tcctcgtcgc tgctcaacgc gatcgcgagc aacgcggcgc
     1921 cgccgatacc gccgaggatc gcgacgccct tggcgctctt ccagaactga ccggccgagg
     1981 ccttgctctc gacgcgggcc acgcccgacg gcgcttgatc gatcccggcg aaccctcgcg
     2041 gggcttcctg gtcggccgcg ccggcgtaca acgtcatcgt caggctggcc ttgccggccg
     2101 gcgggacgtt gacgacctga tccgcgacgt acaggccgtc gccggtttga accgcgatgt
     2161 cgaagtaacc gtacggtagc gccgcgatct tgaaccggcc cttggcgtcg gtgtccgccg
     2221 agcggaagac gctctcgctc gacaggtggt aggcgacgac ggtggcgccg ctgacggcgg
     2281 tcccgctcgg cgtcgagacc tggccgacga gctgcccttg accggcggcc ggcgccggct
     2341 cgaccgtctc ctccgcgagc gctccggcgg cctgaacgag cagcgccagg cccagcggaa
     2401 tcaccgttat cgatctgcgc atcttcggtc tcaccccttg actcggccaa tctccgcgaa
     2461 ctaacatgcg tgcaaccaca cccgacagag gatagcaacg ctcttccctt tcacaaaacg
     2521 gtgcccccct tgacgccacg cacgctcgac atcgagcccg acccgcgcga aacagcgccg
     2581 gtcgaagtcc ggacgcgccc gccgcgcgag catcgcgcgg ctcgtcgttc gccgcggcac
     2641 agccacacgc gcgtgcggat gctctatttc ggtctttcgt cggtttgggg cttcctcgcc
     2701 ggatcggtcg ccgtgctcgt cgcgttcgac gcggtcggcc acccgatcac gctgcgcggg
     2761 gagctgggca cggcgctgtc gatcgccgcg gtgctcgcgg tcgtcggcgg cgtgatcgtc
     2821 tcgatggcgt accgcgagct ggcgggcagg cggtagcgcc gcgcgcctca gccccgcagc
     2881 aggccgtagc gcaggatgca ggccaacgcc tcgacgccgt cgcgccagtt gatcttcttg
     2941 ccctcggcgt aggtccgccc ccagtagctg atgccgactt cgtagatgcg tacgtcgagg
     3001 cgcgagatct tggccgtcac ctcgggctcg aagccgaagc gctcctcacg caggtcgagg
     3061 ccgcgcagca ggtcgccgcg gaacatcttg tagcaggttt ccatgtcggt gagattgagg
     3121 ttggtgacgg cgttcgacac cagggtgagg aaccgattcc cgacgtagtg
//
LOCUS       DASPGN010000384         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1708736, whole genome shotgun sequence.
ACCESSION   DASPGN010000384 DASPGN010000000
VERSION     DASPGN010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708736"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1476)
                     /locus_tag="VD788_11045"
     CDS             complement(<1..1476)
                     /locus_tag="VD788_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76839.1"
                     /translation="MRRLLIPIVLAPALAVGCAQRDKGTASTLAALHDVRPDVEEATV
                     EQGLEQAMQGYRRFLEETPETAMTPEAMRRLADLQLEKQFGIRVGDGKPREMAAAAPR
                     TEMAAPEPAPPPALDAGAAVAGVGQRESDQDFEARTTAESGMLTGSDPVSSPAGPIAD
                     PTGPLEAIALYDRLLTEYPDYEHNDKVLYQKARAYDELGRTEEAIETMERLIGANPHS
                     EHHDEVQFRRGEYFFTRRQYRDAESAYSTIIGRGAGTEYYELALYKLGWTFYKQELYE
                     EALHKYIALLDYKVSIGYDFDQTQAEDDERRVADTYRVISLSFSNLGGPDSVREYFAE
                     FGSRSYEDRVYSNLGEHYLAKLRYDDAAKTYKAFVALYPFHRAAPHFSMRVAEVYAAG
                     GFPKLVLESKKAFAASYGLDSEYWRHFDIDGSPDVLGYLKTNLTDLAGHYHALYQDVA
                     LGEERPANFQEALRWYRAYLTSFPTDPGSPGVNHRLADLLLE"
     gene            complement(1476..3365)
                     /locus_tag="VD788_11050"
     CDS             complement(1476..3365)
                     /locus_tag="VD788_11050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76840.1"
                     /translation="MRAPLRLLGLFLVLAVGLGARPVVGAELRDLYFGEALYHAQQGR
                     YFDALERLDTEIAQYRDLDEPGLDTLHYHINDAEFSVGDFELNYRMHHRAGRAIRAVL
                     EGAVDEAVRNEAAFRLARIHFQKDQLDDALYALDRITGTVPEPIADEVEFLRANIYMA
                     TGRPSEAVDVLERLRADDSLAGFAAYNLGIALLQDGRSEDAIEQLDRAGRLAAGDRAG
                     FAIRDKSNLVLGSILFESTDFERAKQSMDRVRLDGPFSNQALLRAGWSEASAERYDRA
                     LVPWTILVEREPTDFAVQEAMLAVPHAYARLDLHGRAALGYGRALQSFGEQIERVDAS
                     IGSIRQGRFLDALIREESRQDEGWVIRLRGLPDAPETYYLMELMASHDFQTALQNYLD
                     LEDLRSRLIGWKTSLDAFEDIIGLRQENYEPRLPDVDAQFRELDSRIRLRLEQRKHVG
                     DRLQAMLTAPRPEYLATADERIVSERLALIEQQLGDSDDPESLALRRRTARLRGALSW
                     RLETEYHERLTTAHLHLNELNADVDELTRRYDAFVRTRQAATHSYVGYDVQIDRLRQR
                     VGDALERVDLLMSRQGHLIETVAINQLEARRERLVAQQTQARFAVADSYDRAARAQSV
                     EVEGR"
     gene            complement(3369..>3767)
                     /locus_tag="VD788_11055"
     CDS             complement(3369..>3767)
                     /locus_tag="VD788_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76841.1"
                     /translation="VLGIAAELSLLEEKLLYPSGTQVAIFVALAEGDRMRLDAVRLQI
                     DGQLVAHYIYGAKELEALRNGGVQRIYVGNVATGDHRLDVLIDGKREDGKDFSQTGQF
                     VFRKEVKPKLVELTLAAPDSGGTPIALGEL"
BASE COUNT          559 a         1271 c         1269 g          668 t
ORIGIN      
        1 ctcgagaagc agatcggcga gtcgatgatt gaccccgggc gatcccggat ccgtcgggaa
       61 cgaggtgaga tacgcgcggt accagcgcaa tgcctcctgg aagttggctg gccgctcctc
      121 tccgagggcc acgtcctggt agagcgcgtg gtagtgaccc gcgagatccg tcaggttcgt
      181 cttcaggtag ccgaggacgt ccggcgagcc gtcgatgtcg aagtgacgcc agtactcgga
      241 gtccaggccg tagctggcgg cgaatgcctt cttcgattcg agcaccagct tcgggaaccc
      301 gcccgccgcg tatacctcgg cgacgcgcat gctgaagtgc ggtgcggcgc gatggaacgg
      361 atagagcgcg acgaaggcct tgtacgtttt cgccgcatcg tcgtagcgca gcttggccag
      421 gtagtgctcg ccgaggttgc tgtagacgcg gtcctcgtag gagcgactgc cgaactcggc
      481 gaagtactcg cggaccgaat ccggcccgcc gaggttgctg aagctcaggc tgatgactcg
      541 gtaggtatcg gcgacacggc gttcgtcgtc ctccgcctgg gtttgatcga agtcgtagcc
      601 gatcgagacc ttgtagtcga gcagcgcgat gtacttgtgc agggcctcct cgtacagctc
      661 ctgcttgtag aacgtccacc cgagcttgta cagcgcgagc tcgtagtact cggtgccggc
      721 gccccgaccg atgatcgtcg agtaggcgct ctcggcatcg cgatactgcc tgcgcgtgaa
      781 gaagtactcg ccgcgccgga actgcacttc gtcgtggtgc tcggagtgcg gattcgcgcc
      841 gatcaggcgc tccatcgtct cgatcgcctc ttcggtgcgg ccgagctcgt cgtaggcccg
      901 cgccttctga tacagcacct tgtcgttgtg ctcgtagtcc gggtactcgg tgagcagccg
      961 gtcgtacagc gcgatcgcct cgagtggacc cgtcgggtcg gcgatcggac cggccggcga
     1021 gctgacgggg tcgctgccgg tcagcatccc gctctccgcg gtggtgcgcg cctcgaagtc
     1081 ttggtcggac tcccgttggc cgacgccggc gaccgcggcg cccgcgtcga gcgcgggtgg
     1141 gggcgcgggc tccggtgcag ccatttccgt tcgcggcgcg gctgcggcca tctcgcgcgg
     1201 cttgccgtcg ccgacacgta tgccgaactg cttctcgagc tgcaggtccg cgagacgccg
     1261 catcgcctcc ggagtcatcg ccgtctccgg tgtctcctcg aggaagcgtc ggtaaccctg
     1321 catcgcctgc tcgaggccct gctcgaccgt cgcctcctcg acgtccggac gcacgtcgtg
     1381 cagcgccgcg agcgtcgacg cggttccctt gtcgcgctgg gcgcagccga cggcgagtgc
     1441 cggcgcgagc acgatcggta tgaggaggcg gcgcatcagc gtccctccac ctcgacggac
     1501 tgggcgcgcg cggcccggtc gtagctgtcg gcgacggcga agcgggcctg ggtctgctgc
     1561 gccaccaggc gttcccggcg ggcctcgagc tggttgatcg cgacggtctc gatcaggtgt
     1621 ccctgcctgg acatcagcag atcgactcgt tccagcgcat caccgacgcg ctggcgcaac
     1681 cggtcgatct ggacgtcgta gccgacgtag ctgtgcgtcg ccgcctgtcg cgtcctgacg
     1741 aaggcgtcgt accgccgcgt cagctcgtcg acgtccgcgt tcagctcgtt gaggtgcaga
     1801 tgagccgtgg tcaaccgctc gtggtactcg gtttccagtc gccagctgag cgcgccgcgc
     1861 agtcgggccg tacgtcgccg cagtgcgagc gattcgggat cgtcggagtc accgagctgc
     1921 tgttcgatga gggccagacg ctcgctgacg attcgctcgt ccgccgtcgc caggtattcc
     1981 ggtcgcggcg cggtcagcat cgcctgcagg cgatcgccga cgtgcttgcg ctgctcgaga
     2041 cgcaggcgga tccgcgaatc cagctcgcgg aactgcgcgt cgacgtccgg cagccgcggc
     2101 tcgtagttct cctgccgcag gccgatgatg tcttcgaacg catcgaggct cgtcttccag
     2161 ccgatcagcc tcgatcgcaa gtcctcgagg tcgaggtagt tttgcagcgc ggtctggaag
     2221 tcgtgcgacg ccatcaactc catcaggtaa taggtttccg gcgcatcggg caggccgcgc
     2281 aggcggatca cccagccctc gtcctgccgg ctctcctcgc ggatcagcgc gtcgagaaac
     2341 cgaccctgcc ggatgctgcc gatcgaggcg tccacccgtt cgatctgttc gccgaacgac
     2401 tggagcgccc gcccgtaccc cagcgcggcg cggccgtgca ggtccagcct ggcgtacgca
     2461 tgcggcacgg ccagcatcgc ctcctgcacc gcgaaatcgg tcggctcgcg ttcgaccagg
     2521 atcgtccacg gaacgagcgc gcggtcgtac cgctcggccg atgcctcgga ccagccggcc
     2581 cgcagcagag cctggttcga gaacggtccg tcgagacgga cccggtccat cgactgcttc
     2641 gcccgctcga agtcggtcga ctcgaacagg atgctgccga gcacgaggtt cgacttgtca
     2701 cggatggcga aaccggcgcg atccccggcc gccagccgac cggcccggtc cagttgttcg
     2761 atcgcgtcct cggatcgacc gtcctgcagc aatgcgatgc cgaggttgta ggcggcgaac
     2821 ccggccaggc tgtcgtcggc acgcagccgc tcgagcacgt cgacggcctc gctcgggcgg
     2881 cccgtcgcca tgtagatgtt cgcgcgcagg aactcgacct cgtccgcgat cggttcggga
     2941 accgtacccg tgatccgatc cagcgcgtac agcgcatcgt cgagctggtc cttctggaag
     3001 tggatgcggg ccagccggaa tgcggcctcg tttcgcacgg cttcatcgac ggcgccttcg
     3061 agcacggccc ggatcgcgcg ccccgcgcgg tggtgcatcc ggtagttgag ctcgaagtca
     3121 ccgacggaga actcggcgtc gttgatgtga tagtgcagcg tatcgagtcc cggctcgtcg
     3181 agatcgcggt actgcgcgat ctcggtgtcg agccgctcga gcgcgtcgaa atagcgcccc
     3241 tgctgggcgt ggtacagcgc ctcgccgaaa tacagatccc gcagttcggc accgacgacc
     3301 ggacgggccc cgagtcccac ggccaggacc aggaagagtc cgagcaggcg gagtggcgct
     3361 cgcatcgatc acagttcccc gagcgcgatc ggcgtgccgc cggaatcggg cgcggccagg
     3421 gtcagttcca ccagcttcgg cttcacttcc ttgcggaaga cgaattgtcc ggtctgactg
     3481 aagtccttgc cgtcctcgcg cttgccgtcg atgagcacgt cgagccggtg atccccggtc
     3541 gcgacgttgc cgacgtagat ccgttgcacg ccgccgttgc gcagggcctc gagctctttg
     3601 gcgccgtaga tgtagtgcgc gacgagctga ccgtcgatct gcagccgcac ggcgtcgagc
     3661 cgcatccgat cgccctcggc caacgcgacg aagatcgcga cctgcgtccc cgacgggtag
     3721 agcagctttt cctcgagcag gctcagctcc gccgcgatgc cgaggac
//
LOCUS       DASPGN010000385         7246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1473540, whole genome shotgun sequence.
ACCESSION   DASPGN010000385 DASPGN010000000
VERSION     DASPGN010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473540"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1967
                     /locus_tag="VD788_11060"
     CDS             138..1967
                     /locus_tag="VD788_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76842.1"
                     /translation="MTRKRRGCAPIPEYPKFFKLDGDHPYKREVPDGHVPYRARRVRG
                     AQVVYFNFALAREMGLIGARHPDRLNAKLRRSIVDTFGLRIINEYDLLSGVRVPPRDR
                     LDGSYMATRYLALQHPGRTGKTSGDGRSIWNGTVTHRGVTWDVTSCGTGVTKLCPATA
                     ESDRYYKTGNWRTDYGCGTSTVLEGLVSTLMSETFARNEIATERVLAVLELPDGFGIT
                     VRAGCNLLRPSHFFVHLKQARLASLAAAIDCFIERETENGRFPRLDLPAERYRHLAAY
                     IADTFARVAATFEREYIFCWLDWDGDNILADGGIIDYGSVRQFGLYHREYRFDDGPRW
                     STTLPEQRRKARQIVRTFAQIRDYLLTGRKPALASLSRDPVLRRFDHGFARTRDALLL
                     RDVGFDRAAADALHARAPRQVEAFRRVHHWFERSRSARGPHHVGDGISWNAIFSTRDL
                     LRELPGRLLAGGAELPPELLIEIALSTFATHRDRAMTPQRIRQAIRFRQAYRGLIETA
                     ARLTGRSVPLVLADVAARAAILNRYDRITGDGILHAAERLHRGRRRLGPEGVYAVVDG
                     VARYQTRLPERAAPADARPPRVAGAERIFDGIVELLADCRHGL"
     gene            complement(1955..2173)
                     /locus_tag="VD788_11065"
     CDS             complement(1955..2173)
                     /locus_tag="VD788_11065"
                     /inference="COORDINATES: protein motif:HMM:NF040329.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6116 family protein"
                     /protein_id="HXV76843.1"
                     /translation="MIDWLTRWASGLRFPTLLAVTAALFLVDLVLPDLIPFVDEVLLA
                     LLTVLFAALRRRTGRDRAAASTHDRHRP"
     gene            complement(2238..2570)
                     /locus_tag="VD788_11070"
     CDS             complement(2238..2570)
                     /locus_tag="VD788_11070"
                     /inference="COORDINATES: protein motif:HMM:NF039419.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HXV76844.1"
                     /translation="MRKIAIVLATLLAVSWTAAALAEEAITLQGKVSCAMCVMKEEGA
                     KDCQNVLQVADGDGTVDYYLVKNEVAEEFGHACDGAKAVQVTGTVAEKDGKRWLTATK
                     MVPVVKKA"
     gene            complement(2660..3715)
                     /locus_tag="VD788_11075"
     CDS             complement(2660..3715)
                     /locus_tag="VD788_11075"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXV76845.1"
                     /translation="MIKSQLEAGTVGQRPAINAVVRGVTRVVSGMTPQERSFCAYMLM
                     GPSGTGKSHLIGMLARVIHGDERRVVVADCTQFVHGDPWMVFAAQLAPLFDGSVIDHG
                     ASLREAPPLSIVRIEYLERGPEEISRGLGVALETGQLVLPEGRRGSLRNCLVFLTSTL
                     CSKEILDEAPRIGFSGAQDDDDEEMHDRIYETCRQQAEQRFGGNLIGRLDALVVFHRF
                     QHEHLSEILDHRLDRLNAWLAPRRIRCSFLPAAKVLLLERGGRDLRLGARALIRTHQR
                     LVEFPLADMMLSGRIVAGTHVRIDRRNADDYLHFDVTEDRSAATSIGAREVPVSWASR
                     RASATPRAGDSRPSSAH"
     gene            complement(3778..4086)
                     /locus_tag="VD788_11080"
     CDS             complement(3778..4086)
                     /locus_tag="VD788_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76846.1"
                     /translation="MFWNEPSLYGASFPYRDVTTPVQHPLMGVTGLPWQQFPRFVPPI
                     YGFNQPFFNMPIGNTPMVNPMIYPQFANPFLRPFDVPQMRFDVPRWDLPLHNLYRPFF
                     "
     gene            complement(4154..4354)
                     /locus_tag="VD788_11085"
     CDS             complement(4154..4354)
                     /locus_tag="VD788_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76847.1"
                     /translation="MTTTTKTTVKGSPTVDAPESPTREEIQAAYQIHTLSRILYNHMA
                     NAQSWSAMMPTNPGYWGYPYGW"
     gene            complement(4386..4910)
                     /locus_tag="VD788_11090"
     CDS             complement(4386..4910)
                     /locus_tag="VD788_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXV76848.1"
                     /translation="MNHQVSRALDEVRDLYRQVVGTPAPEIQPVSYASFPPGVDPLDH
                     ALREVKQLQKISEQLKFAPRLAVWAPPADTYSGKDEFVVRVEVPGITRDDLKVFVGAG
                     ECIVRGERKPPSNADELPMTIERSWGQFERRFVLPTGCQQDRVSARCHDGVLEIRIAA
                     GERTQPVETKVEID"
     gene            5049..5501
                     /locus_tag="VD788_11095"
     CDS             5049..5501
                     /locus_tag="VD788_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXV76849.1"
                     /translation="MTANTTAPDVGRRLGAARLLRGLSQATVARRAGIAASYLSRVEN
                     GKVQPTYPTLMRIVRALRVDLGQVVGESDGGPTARRCPVSPDGRCLCELIRPQAEIWR
                     DPHGEHYSARELQLIRSLALWLRAAKPERLRALEVLIEDLTRAALDPR"
     gene            complement(5498..6553)
                     /locus_tag="VD788_11100"
     CDS             complement(5498..6553)
                     /locus_tag="VD788_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76850.1"
                     /translation="MRDKPRTCAVLALTALAVGCGGERAGSVMSDDGDGAATAAVART
                     KVFETEPLPIDRIYHSMQGPFDRIQVDHTDLDWVTAVHTEVIDADTGEPMGGEFFCHS
                     QVQLLNGTRLMVMATGADTIRFPQGFAMPVSSIMRGVPAEQRALTFLGMVLNNHETEI
                     DRRGKVRTTVEYLRDVDLGQAGPPKKLFRADLTMFVEDLEEYVPDDAGLEPNDDVTTH
                     CALVGELPQHWIVPPGKQKTRYRYTDFVPVDGRVHFGWVHLHNYGVYMRLTDVTTGDV
                     LFQADVENEPDRDQIRRISNYSSEEGFPLYRDHVYEIEALYDNTTGHDVDAMAMMVLY
                     YHPDGNVDITYPAPPAG"
     gene            6659..7069
                     /locus_tag="VD788_11105"
     CDS             6659..7069
                     /locus_tag="VD788_11105"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXV76851.1"
                     /translation="MDRRPATTGIGAGLLLCALAAAGCGERAEPAPRDGGPALGERAA
                     SDAMLLYRNEGCPMCHGELGAGVDGVGPPLRDLAAYWDRERLIAYLADPEAFRQANPD
                     FDQRHEEEYGLEMPPYDYLAPDKRGLLADWLLAR"
BASE COUNT         1113 a         2467 c         2470 g         1196 t
ORIGIN      
        1 agatgatcga ccagaacagg acgctggcca gttttcgacg cacgtcgccg attgtctcac
       61 gagcgccggt tggcttccgc ggcgtcgaaa acctatcttt ggcggtggga tttcgggggc
      121 gtcgagcaag ggacccgatg acgaggaaac gacgcggctg tgcgccgatt ccggaatacc
      181 cgaagttctt caagctcgat ggcgatcacc cgtacaaacg ggaagtgccc gacgggcatg
      241 tgccctaccg cgcccgccgc gtgcgcggcg cgcaggtggt gtatttcaac ttcgcgctgg
      301 cgcgcgagat gggcttgatc ggtgcgcgcc accccgaccg gttgaacgcg aagctccggc
      361 gatcgatcgt cgacaccttc ggcctgcgca tcatcaacga gtacgacctg ctcagcggtg
      421 tccgcgtccc gccacgcgat cggctcgacg gcagctacat ggccacgcgc tacctggcgc
      481 tgcagcatcc cggacggacc ggcaagacgt cgggtgacgg gcggagcatc tggaacggca
      541 cggtcacgca tcgcggcgtc acctgggacg tgacaagctg cggcaccggc gtgacgaaat
      601 tgtgtccggc gaccgccgag agcgatcgct actacaagac cggcaactgg cgcaccgact
      661 acggctgcgg cacgtcgacg gtgctcgagg gcctcgtcag cacgctgatg agcgagacct
      721 tcgcgcgcaa cgagatcgcg accgagcgcg tgctggcggt gctcgagctg cccgacggat
      781 tcggtatcac ggttcgcgcc ggatgcaacc tgctgcgtcc ttctcatttc ttcgtgcacc
      841 tgaaacaggc gaggctggcc tcgctcgccg cggcgatcga ctgcttcatc gagcgcgaga
      901 cggaaaacgg acgctttccc cgtttggacc tgccggccga acggtaccgc cacctcgccg
      961 cgtacatagc ggacaccttc gcacgcgtcg cggcgacctt cgagcgcgag tacatcttct
     1021 gctggctcga ctgggacggc gacaacatcc tggcggacgg cgggatcatc gactacggct
     1081 cggtgcggca gttcgggctg tatcaccgcg agtaccgctt cgacgacggc ccacgctggt
     1141 cgacgacgct gcccgagcag cgacgaaagg cgcgtcagat cgtccggaca ttcgcccaga
     1201 tccgcgacta cctgctgacc gggcgcaagc cggcgctcgc ctcgttgtcg cgcgacccgg
     1261 tgctccgccg cttcgaccac gggttcgcgc ggacacgcga cgcgctgttg ctgcgggacg
     1321 tcgggttcga ccgggcggcg gccgacgcgc tccacgcacg cgccccacgg caggtcgagg
     1381 cgtttcgccg agtgcaccat tggttcgagc gctcgcggtc ggcgcgcggc ccgcatcacg
     1441 tcggcgacgg catcagctgg aacgcgatct tcagcacgcg cgacctgctg cgcgagctac
     1501 cgggacgact gctggccggc ggagcagagc tgccgccgga gctcttgatc gagatcgcgc
     1561 tgtcgacctt cgccacccac cgcgaccgtg cgatgacgcc gcaacggatc cgtcaggcga
     1621 tccgcttccg gcaggcctat cgcggcctga tcgaaaccgc cgcccggctg accggccggt
     1681 cggtaccgct cgtgctggcc gacgtggcgg cccgcgccgc gatcctcaac cgctacgatc
     1741 ggatcaccgg ggacggcatc ttgcacgccg ccgagcgact gcatcgcgga cgccgtcgcc
     1801 tcggccccga gggcgtctac gcggtcgtcg acggcgtcgc gcgctaccag acccgtctgc
     1861 ccgagcgtgc cgcgccggcg gacgcgcggc cgccgcgggt cgcgggagcg gagcggatct
     1921 tcgacgggat cgtcgagctg ctggcggatt gccgtcacgg tctgtgacgg tcgtgggtgg
     1981 aggcggcagc tcgatcgcga cccgtccgcc ggcgcagcgc ggcgaacagc acggtcagga
     2041 gcgcgagcag gacctcgtcg acgaagggaa tcaggtcggg gaggacgaga tccacgagaa
     2101 acagcgcggc ggtgacggcg agcaaagtcg ggaagcgcag cccggaggcc cagcgcgtca
     2161 accagtcgat cacggtgtcc ccgccggggc acgccgccgg tcggcggcgc gtcccgtccc
     2221 cagaaggtcg actgggctca ggctttcttc acgaccggta ccatcttggt cgccgtcagc
     2281 caccgcttgc cgtctttctc ggcgacggtc ccggtcacct gcacggcctt ggcgccgtcg
     2341 caggcatggc cgaattcctc ggcgacttcg ttcttgacca ggtagtagtc gacggtgccg
     2401 tctccgtcgg ccacctgcag cacgttctgg cagtccttgg ccccttcttc cttcatcacg
     2461 cacatggcgc agctcacctt gccctgcagc gtgatcgctt cctcggccaa agcggccgcc
     2521 gtccaactca cggccagcaa ggtggccaac acgatcgcga tcttcctcat cgtaatcctc
     2581 cgcgctcgaa agttgatgga gtcgccatcc gggcattgta gccgatgacg gcaacgccgt
     2641 cggggccgcc cggggagacc taatgcgccg agctcggccg cgagtcgccg gcgcgcggtg
     2701 tcgccgaggc gcggcgactc gcccacgaga cggggacctc gcgcgcgccg atcgacgtcg
     2761 ccgccgaccg atcctccgtg acgtcgaagt ggaggtagtc gtcggcgttc cgccggtcga
     2821 tccgcacgtg cgtcccggcg acgatccggc cgctcagcat catgtcggcc agcggaaact
     2881 cgaccagtcg ctggtgcgtg cggatcaacg cgcgcgcccc gagccgcagg tcgcgtccgc
     2941 cccgttcgag cagtaacacc ttcgccgccg gcaggaagct gcagcggatg cgccgtggag
     3001 cgagccaggc gttgagacgg tccaggcggt ggtcgaggat ctcggacagg tgctcgtgct
     3061 ggaagcggtg gaagacgacc agcgcgtcga gcctgccgat cagattcccg ccgaagcgct
     3121 gttccgcctg ctgccggcag gtctcgtaga tccgatcgtg catctcctcg tcgtcgtcgt
     3181 cctgcgcgcc gctgaagccg atgcgcggcg cttcgtcgag gatctccttg gagcacaggg
     3241 tgctggtgag gaacaccagg cagttgcgca ggcttccgcg acgcccctcg ggcagcacca
     3301 gctggccggt ctcgagcgcg acccccagcc cgcgagaaat ctcctccggc ccgcgctcga
     3361 ggtactcgat ccgcacgatc gacagcggcg gcgcttcgcg cagcgacgca ccgtggtcga
     3421 tcaccgatcc gtcgaacagc ggcgcgagct gggcggcgaa gaccatccac ggatcgccgt
     3481 ggacgaactg cgtgcagtcg gccacgacga cgcgccgctc gtcgccgtgg atcacgcgcg
     3541 ccagcatccc gatcagatgc gacttacccg tcccgctcgg ccccatcaac atgtaggcgc
     3601 agaaagagcg ttcctgcggc gtcataccgc tgaccacgcg cgtcacccct cgaacgacgg
     3661 cgttgatcgc cggccgctgg ccgacggtgc cggcctcgag ctgtgacttg atcaactcgg
     3721 agaacatcgg atgaccgctg cggacgcggc cggcgtcgcc ggccgcgtcg tttcgcgcta
     3781 gaagaaggga cgatagagat tgtgcagcgg caggtcccac cgcgggacgt cgaatcgcat
     3841 ctgcggcacg tcgaacggtc gcaggaacgg attggcgaac tgcgggtaga tcatcggatt
     3901 gaccatcggc gtgttgccga ttggcatatt gaagaagggt tggttgaagc cgtagatcgg
     3961 cggcacgaat cgcgggaact gctgccatgg caggccggtg actcccatca acgggtgctg
     4021 caccggagtg gtgacgtccc tgtacgggaa gctggctccg tacagactcg gttcgttcca
     4081 gaacatcggc tcctcctttg ttgtgggtcg gcgccggggc tgccggcgcg atccggttgt
     4141 ggccgcagac gtcttaccag ccgtacgggt aaccccagta gcccgggttc gtcggcatca
     4201 tcgcgctcca gctctgtgcg ttcgccatgt ggttgtagag gattcgcgag agggtgtgga
     4261 tctggtacgc ggcctggatt tcctcgcgcg tcggcgattc gggcgcgtcc acggtcggtg
     4321 aacccttgac ggtcgtcttg gtcgtcgtcg tcatctctct ctcctgccgg ctcgtgccgt
     4381 gtcgatcagt cgatctcgac cttggtttcc accggctgcg ttcgctcgcc ggcggcgatg
     4441 cggatctcga gcacaccgtc gtggcagcgg gccgagacgc ggtcctgctg gcagccggtc
     4501 ggcaggacga accgtcgctc gaactgcccc cagcttcgtt cgatcgtcat cggcagctcg
     4561 tcggcgttcg acggtggctt tctctcgccg cggacgatgc actcgccggc cccgacaaac
     4621 accttgagat cgtcacgcgt gatgccgggc acctcgacac ggacgacgaa ctcgtccttg
     4681 ccggagtagg tgtcggcggg cggggcccac acggcgagac gcggggcgaa cttcaactgc
     4741 tcggagatct tctgcaattg cttgacctcc cgcagcgcgt gatccaacgg atcgacgccg
     4801 ggcgggaacg acgcgtacga caccggttgg atctcgggcg ccggcgtgcc gactacctgc
     4861 cggtacagat ccctcacctc gtcgagggct cgactgacct ggtggttcat ctcaaacctc
     4921 ctgaccctgt ggccgttcga tctcttgtgg agtccgggtg gtggcggcag cgtagttcgc
     4981 gcttgatcgc cggttcaata tctgctaaca gaaacccagc acagggatgt tcacgttcgg
     5041 ggcgcaactt gactgccaat acaacagcgc cggacgtcgg acgacggctg ggcgccgccc
     5101 gtctgctgcg ggggctgtcc caggctaccg tcgcgcgccg cgccggaatc gccgcctcct
     5161 acctgtcccg cgtcgagaat ggcaaggtcc agccgaccta tccgacgttg atgcgaatcg
     5221 ttcgtgcgtt gcgggtcgac ctcggccagg tggtcggcga atccgacggc ggcccgaccg
     5281 cgcggcgctg cccggtctca ccggacggcc gctgcctgtg cgagctgatc cgtccacagg
     5341 ccgagatctg gcgcgatccc cacggggagc actactcggc gcgcgagctg caattgattc
     5401 gcagcctggc gctctggctc cgcgcggcca agccggagcg gctgcgcgcg ctcgaggtgc
     5461 tgatcgagga cctgacccgc gccgcgctcg acccgcgcta accggccggt ggcgcggggt
     5521 aggtgatgtc gacgttgccg tccgggtggt agtagagcac catcatcgcc atcgcgtcga
     5581 cgtcgtgccc ggtcgtattg tcgtagagcg cctcgatctc gtagacgtgg tcgcggtaga
     5641 gcgggaaacc ttcctcgctg gaatagtttg agatccgtcg gatctggtcg cggtcgggct
     5701 cgttctccac gtcggcctgg aacagcacgt cgccggtggt cacgtcggtc agccgcatgt
     5761 agaccccgta gttgtgcagg tggacccagc cgaagtgcac ccggccgtcg accggaacga
     5821 agtcggtgta gcgatagcgc gtcttctgct tgcccggcgg gacgatccag tgctggggca
     5881 gctcaccgac cagcgcgcag tgggtcgtca cgtcgtcgtt gggctcgagg ccggcgtcgt
     5941 ccggcacgta ttcttcgagg tcttcgacga acatcgtcag gtcggcgcgg aacagcttct
     6001 tcggcggccc ggcctggccc agatcgacgt cgcgcaggta ctcgacggtg gtgcgcacct
     6061 tgccccgtcg atcgatctcg gtctcatggt tgttcagcac catgccgaga aaggtcaacg
     6121 cccgctgctc ggcgggcacg ccgcgcatga tcgacgagac cggcatggcg aagccttggg
     6181 ggaagcggat cgtgtcggcc ccggtggcca tcaccatcaa ccgcgtgccg ttcagcagct
     6241 ggacctggct gtggcagaag aactcgccgc ccatcggctc gccggtgtcg gcgtcgatca
     6301 cctcggtgtg gacggcggtg acccaatcga ggtcggtgtg gtcgacctgg atccggtcga
     6361 acgggccctg catcgagtgg tagatccggt cgatcggaag cggctcggtc tcgaacacct
     6421 tggtgcgggc cacggccgcg gtggccgccc cgtcgccgtc gtcggacatc accgagcccg
     6481 cccgctcccc gccgcagccg accgcgagcg cggtgagcgc gagcacggcg caggtcctcg
     6541 gtttgtcgcg catacccgtt ggtagattct acggtaggtc gcgtgcgcct tcaaagccga
     6601 tcgggctccc ttataatccc gcggaacggt cacgacgggg acggagggct cgagacggat
     6661 ggaccgacga ccagcgacga cggggatcgg cgcgggcctg ctgctgtgtg cgctcgccgc
     6721 ggccggctgc ggcgagcggg cggagccggc accgcgcgac ggcgggcctg cgctcggcga
     6781 gcgcgccgcg tcggacgcga tgctgctcta tcgaaacgag ggctgcccga tgtgtcacgg
     6841 cgagctgggg gccggtgtcg acggcgtggg cccgccgctg cgcgacctcg ccgcgtactg
     6901 ggaccgcgag cggctgatcg cctacctggc agaccccgag gcgtttcgcc aggccaaccc
     6961 cgacttcgac cagcgacacg aagaggaata cggtctcgag atgccgccct acgactatct
     7021 cgcgcccgac aagcgcgggc tgctggccga ctggctgctc gcgcgctgaa ccgggcccgc
     7081 gcgcggcgcg ggaccccggc ccgcgtcact ccgcgatcgg ctcgagcgtc aacgggtcgc
     7141 gcagcggaag ctcggtcgct ttgccgccgt agaactgcgc catcgacgcg cgctcgtcgg
     7201 cgtcaccctt gacgatctcg tcccacttgc cgacgatcag cagcac
//
LOCUS       DASPGN010000386         5687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1123617, whole genome shotgun sequence.
ACCESSION   DASPGN010000386 DASPGN010000000
VERSION     DASPGN010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123617"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VD788_11110"
     CDS             complement(<1..757)
                     /locus_tag="VD788_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76852.1"
                     /translation="MPDASGPGRRDRDKLLGLLEGGLVVVVLTAPLPFGAVTVGGRAW
                     LEILALALLGLWCVRAVRRPTVLPPRAVTAGLLGVVGLGLCQAIPLPDVVVSVLSPQA
                     REIHRVATPPAAIRRAEAELLPSAEVELDRAAALSVDPGLTASATRTGAALTGLLLIA
                     TTVAATRGARSIALALLGSAAFQSIYGLLVLVSGHDRIWQVPKRYFLDSATGTFVNRN
                     HFAGLLAMSLACGLALILASVERPGGNRRPGRRW"
     gene            complement(763..3009)
                     /locus_tag="VD788_11115"
     CDS             complement(763..3009)
                     /locus_tag="VD788_11115"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HXV76853.1"
                     /translation="MLRDEINLRDQWRILLRRRWTIYLAVASVTLVALFASFAMTPLY
                     RATATLQIERRNPDILTFRDLSQVDYSWAAYNDFYQTQYKLIGGAPVARNAADRLGLA
                     SHPDFDTGKRRPGLLMRLRSLVPRNRPAVETDPRDRLAQRILAGLDVSPIRNSHLVTI
                     AWTSDNPELAAMVANSVADSYVQLNIESQYSTSGQAEEFLVDQIANLKREITEIESKL
                     QDYGESKRIVSIDDTENLTLQALKEISAKHTEAQTTLARAEAAYHAVRSAAPEALPEV
                     MNSPLISRLREEYAGLEAQVSEQSERFGEDWPVLQTLTTKLRQAERRLAIETELISQQ
                     VRAGAEADYDRARNEVQNLEALLDAQESAAQRLKRDAVEFANLQSEVNKKRETLNALI
                     SRQNEVSLSTRLKDLDATSGNIRIIERAKVPVAPFRPNPLLNVILGLGFGIGLGVALA
                     IMLDHLDNTINSVDELRELVSLPVLATIPRHGAGGSPLGRVRRTRPATLSATLDLVAH
                     RDSKAGVAEAYRDLRTSILLSNPGQPPRTILVTSAVPEEGKTATSINLAVVLAQLGAR
                     VVMVDTDLRRPRLHHALEVRNDRGVSNYLSGLEPDPARLAAPTGIDNLDLVPSGPIPP
                     NPSELLNSPTFGRFARILLDGGYDHVVFDSPPVLSVSDSVIIANLVDTRVLVVRAGRT
                     AKHSVRLAVDKLRQAGDRPLGLVLNDVDPGTDGSTHYGYRYYGREDRRPTSPGVAARE
                     HASGADVG"
     gene            complement(3026..3964)
                     /locus_tag="VD788_11120"
     CDS             complement(3026..3964)
                     /locus_tag="VD788_11120"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXV76854.1"
                     /translation="MRPSVRCWLIPIVTLGIAATDGAVGETPADRTEEPLRLEARPFA
                     EGTPVVPPTAALAGGSDYRIGRQDLLEIAVFDVEELDQTVRVADDGSITMPLLGKLRV
                     AGLTKFELERMIAEQLDDRFIRDPQVTVFIKEYESKKVAVSGAVKNPGTYEMLGQKTI
                     LEMLSEAGGLDQDLGKEIIIFRKPVGEPTRRLAVDLDRLVYRAEPELNLIVEPGDIIY
                     VPAVEKIRIFVSGAVRNPDLYEVPRDEPVTVLKAVTLAGGVTDRAAEKKIQIIRNDEN
                     GERTILLVNLRKVKQGRVEDPLLRKDDIVLVPESFF"
     gene            complement(3982..5211)
                     /locus_tag="VD788_11125"
     CDS             complement(3982..5211)
                     /locus_tag="VD788_11125"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXV76855.1"
                     /translation="MLAIAVLALSPAQATSKLEPPDLDRYLRWGFLRARPGLELSNLG
                     YDNNILLTPEETRVGDYTATLSPKLDGMILFGSRAFLTFVQRFDYTAYINHTDQNFLD
                     NTTLSRLTLPFRSRQIGLFVDAGYVIDHQRPIDLEGARPRTENASGGIGLIVAPGWRT
                     EIELGRRLRVIEYTDDDFNALVGPSVGDRLDRRENGTTLDLSYQLRGRTKLLITGLWQ
                     EIEFAEPFTVGDLSFTRDTDEWRAMAGLEFGEGGPLVGSFRLGWDRIVPRSPILEVFS
                     QPVGQASLRYRLNSRTRFELETERLPGFAVYGANSYYLLWNARFGTIYYFSRLIGIEA
                     GVSAGRLTFPDSIDLLGREDELRSYDLGTRFRLMENSIGRRVEYGFKVRYYDRTSTVP
                     EFDRSATTVGFDAALGF"
     gene            complement(5266..>5687)
                     /locus_tag="VD788_11130"
     CDS             complement(5266..>5687)
                     /locus_tag="VD788_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76856.1"
                     /translation="GVDRLDAFASPVLEANRLRQRSRANAALGQHLAAVADAERAQRL
                     APDDPSLTELLGDQLAAAGDAGRAIETLRAALAQLARGGGNPLARARIYRKIGQAEES
                     RGQPANAYDAYRIAVELAREPIAASRLARMREDAGMP"
BASE COUNT          904 a         1938 c         1909 g          936 t
ORIGIN      
        1 gccagcggcg ccccggtcgc cgattcccgc ccggccgctc gacgctcgcg agtatcagcg
       61 ccaggccgca ggcgagcgac atcgcgagca gcccggcgaa gtggttgcgg ttgacgaagg
      121 tcccggtcgc cgaatcgagg aagtaccgct tgggaacctg ccagatgcgg tcgtggcccg
      181 agacgaggac cagcagcccg taaatcgact ggaacgccgc ggagccgagc agcgcgagcg
      241 cgatcgagcg ggcgccacgg gtggcggcga cggtcgtcgc gatcagcaac aggccggtca
      301 gcgcggctcc ggtcctcgtg gccgaggcgg tcaagcccgg atcgaccgac aatgccgcgg
      361 cccgatcgag ctccacctcg gccgatggca gcagttccgc ctcggcgcgg cgaatcgccg
      421 ccggtggggt cgcgacgcga tggatctccc gcgcctgcgg ggagaggacg gagacgacga
      481 cgtccggcag cgggatcgcc tgacacaaac ccagtccgac cacccccaac aggccggcgg
      541 tcaccgccct cggcggtagc acggtcggcc gccggacggc acggacgcac cacaacccga
      601 gcagcgccaa cgcgaggatc tcgagccagg cccgaccgcc gaccgtcacc gccccgaacg
      661 gcagcggcgc cgtcagcacg acgacgacca gaccgccttc gaggagaccc agcagcttgt
      721 ctcggtcccg acgcccgggg ccgctcgcgt cgggcaagct cgtcagccga cgtcggcgcc
      781 ggacgcgtgc tctcgcgccg cgacgccggg ggaggtcggc cggcgatcct ctcgaccgta
      841 gtagcggtag ccgtagtgtg tcgagccgtc cgtccccggg tcgacgtcgt tgagcaccag
      901 ccccaacggc cggtcaccgg cctgccgcag cttgtcgact gccagccgga ccgagtgctt
      961 cgccgtccgg cctgcccgaa ccaccaggac gcgcgtatcg accagattcg cgatgatcac
     1021 cgagtccgag acggacagca ccgggggcga gtcgaacacg acgtgatcgt agccgccgtc
     1081 gagcaggatc cgagcgaatc gtccgaaggt gggcgagttg agcaattccg acggattggg
     1141 cgggatcgga ccgctcggta ccaggtcgag gttgtcgatc ccggtcggcg ccgccagccg
     1201 ggccggatcc ggctcgagcc ccgacaggta gttgctgacg ccgcgatcgt tgcggacctc
     1261 gagcgcgtgg tgcaatcgcg gacgtctgag atcggtgtcg accatcacca cccgcgcccc
     1321 gagctgggcc agcaccaccg ccaggttgat cgacgtcgcg gtcttgccct cttcggggac
     1381 ggcgctcgtg acgaggatcg tccgcggcgg ctgtccggga ttcgacagca ggatcgacgt
     1441 gcgtagatcg cgataggcct cggccacgcc cgccttgctg tcgcgatggg cgacgaggtc
     1501 caacgtcgcc gacaacgtcg ccggccgcgt tcgtcgtacg cgtccgagcg gtgagccgcc
     1561 cgcgccgtgc cgcggaatcg tcgccaggac cggcaacgag accaactcgc gcagctcgtc
     1621 gacggagttg atcgtgttgt cgaggtgatc gagcatgatc gccagcgcga ctcccagacc
     1681 gataccgaaa cccaggccga ggatcacgtt gagcagcggg ttcggtcgaa acggcgcaac
     1741 cgggaccttg gcccgctcga tgatccggat gttgccggac gtggcgtcga ggtccttgag
     1801 cctcgtcgac agcgacacct cgttctggcg cgagatcagc gcattcagcg tttctcgctt
     1861 cttgttcacc tcgctttgca ggttggcgaa ctcgaccgcg tcacgcttca gccgttgggc
     1921 cgccgattcc tgcgcatcga gcagggcctc gaggttctgc acttcgttgc gcgccctgtc
     1981 gtagtcggcc tcggcgccgg cccgcacctg ctgcgagatc agctcggtct cgatcgcgag
     2041 acggcgctcg gcctggcgta gcttggtggt caacgtctgc agcaccggcc agtcctcgcc
     2101 gaatctctcc gactgctcgc tgacctgggc ctcgagccca gcgtactcct cgcgaaggcg
     2161 cgagatcagc ggcgagttca tcacctccgg aagcgcctct ggcgccgcgc tgcgcacggc
     2221 gtggtacgcc gcctcggccc gggccagcgt cgtctgcgct tcggtgtgct tggcgctgat
     2281 ctccttcagc gcctgcagcg tcaggttctc cgtgtcgtcg atcgagacga tccgcttcga
     2341 ctcgccgtag tcctgcagct tggactcgat ctcggtgatc tcgcgcttca ggttggcgat
     2401 ctggtcgacg aggaactcct cggcttggcc gctggtcgag tactgcgatt cgatgttgag
     2461 ctggacgtac gagtcggcga cgctgttggc caccatcgcg gccagctcgg ggttgtccga
     2521 cgtccaggcg atcgtcacca ggtgcgagtt ccgaatcggg gagacgtcga gcccggccaa
     2581 gatccgttgg gccaggcggt cgcgcggatc ggtctcgacc gcgggccggt tgcgcggcac
     2641 gagcgaccgc aggcgcatga gcagccccgg tctccgcttg ccggtgtcga agtccgggtg
     2701 gctggccagc ccgagtcgat cggccgcgtt ccgcgcgacc ggtgcgccgc cgatcaactt
     2761 gtactgggtc tgatagaagt cgttgtacgc cgcccaggaa tagtcgacct gcgacaggtc
     2821 gcggaaggtg aggatgtcgg ggttccgtcg ttcgatctgc agcgtcgcgg tcgcccgata
     2881 cagcggcgtc atcgcgaagc tggcgaacaa cgcgacgagc gtcaccgacg caacggccag
     2941 gtagatcgtc cagcgccggc ggagcaggat ccgccactgg tcgcgcaggt tgatctcgtc
     3001 tctcagcatg tcgggacccc gatcctcaga agaacgactc cgggacgagg acgatgtcgt
     3061 ccttgcgcag caacgggtct tccacccgtc cctgcttcac ctttcgcaga ttgaccagca
     3121 ggatcgtccg ttctccgttc tcgtcgttgc ggatgatctg gatcttcttc tcggcggcac
     3181 ggtcggtcac cccgccggcc aacgtcaccg ccttcagcac cgtcaccggc tcgtcgcgcg
     3241 gcacctcgta caggtccggg ttgcggaccg cgccgctgac gaagatgcgg atcttctcga
     3301 ccgccggcac gtagatgatg tcacccggtt cgacgatcag gttgagctcg ggttcggcac
     3361 gatagaccag tcgatcgagg tcgacggcca ggcgccgcgt cggctcgccg acgggcttgc
     3421 ggaaaatgat gatctccttg ccgagatcct ggtcgagccc gcccgcctcc gacagcatct
     3481 cgaggatcgt tttctgcccc agcatctcgt aggttccggg gttcttcacc gcgccgctga
     3541 cggcgacctt cttggattcg tactccttga tgaagacggt cacctgcggg tcccggatga
     3601 agcgatcgtc cagttgctcg gcgatcatcc gctccaactc gaacttggtc aggccggcca
     3661 cccgcagctt gccgagcagc ggcatcgtga tcgagccatc gtcggcgacc cgcaccgttt
     3721 gatccaactc ctcgacgtcg aacaccgcga tctcgagcag atcctggcgc ccgatccgat
     3781 agtcggaccc gccggcgagc gccgccgtcg gtggaacgac cggcgttcct tcggcgaacg
     3841 ggcgcgcctc gagccgcagc ggctcctccg tcctgtcggc cggggtctcg ccaaccgcgc
     3901 cgtcggtggc cgcgattccc agggtcacga tcgggatcag ccagcagcga acggagggtc
     3961 tcatgcttgt acctcgaccg ctcagaaacc gagcgccgcg tcgaagccga cggttgttgc
     4021 gcttcgatcg aactccggca ccgtcgacgt acggtcgtag tagcgtacct tgaaaccgta
     4081 ctcgacgcgc cgtccgatcg agttttccat caaacggaaa cgcgtaccga ggtcgtagga
     4141 acgcagctcg tcctcgcgac cgagcaggtc gatcgagtcg ggaaaggtca gccttcccgc
     4201 gctgacaccg gcctcgatcc cgatcagccg actaaaatag tagatggtgc cgaaccgcgc
     4261 attccacagc aggtagtagc tgttggctcc gtagacggcg aaccccggca gtcgctcggt
     4321 ctccaactcg aagcgggtgc gggagttcag ccggtagcgc agcgacgcct gcccgacggg
     4381 ctgcgaaaaa acctccagta tcggcgaacg cggcacgata cgatcccagc cgagccggaa
     4441 cgaaccgacc agcggcccgc cttcgccgaa ctcgagcccg gccatcgccc gccactcgtc
     4501 ggtgtcgcgt gtgaacgaca ggtcgccgac ggtgaacggc tcggcgaact cgatctcctg
     4561 ccacaggccg gtgatcaaca gcttggttct gccgcgcagc tgataggaca gatcgagcgt
     4621 cgttccgttc tcccgccggt cgagtcgatc gccgaccgac ggaccgacca gcgcgttgaa
     4681 gtcgtcgtcg gtgtattcga tgacccgcag gcgacgtccg agctcgattt cggtgcgcca
     4741 gcccggcgcg acgatcaacc cgatcccgcc gctcgcgttt tcggtgcgcg gtcgcgcgcc
     4801 ctcgaggtcg atcggtcgct ggtggtcgat gacgtagccg gcgtcgacga acagtccgat
     4861 ctgccggctg cggaacggga gggtcagtcg cgacagcgtg gtgttgtcga ggaagttctg
     4921 gtcggtgtgg ttgatgtagg ccgtgtagtc gaaacgctgg acgaaggtga ggaacgctcg
     4981 actgccgaac aggatcattc cgtcgagctt gggcgacagt gtggcggtgt agtcaccgac
     5041 ccgcgtctcc tcgggcgtca acaggatgtt gttgtcgtac cccaggttcg acagctcgag
     5101 ccccgggcgc gcgcgcagga agccccagcg caggtagcga tcgaggtccg gcggttcgag
     5161 cttgctcgtc gcctgtgccg gggacagcgc caagacggca atcgccagca atgacgcgag
     5221 tctactcatt ggacgaaggg agtctaggag tcatcccggc gacggtcaag gcatgccggc
     5281 gtcttcgcgc atgcgcgcca gcctggacgc cgcgatcggt tcgcgggcga gctcgaccgc
     5341 gatgcggtag gcgtcgtatg cgttcgccgg ttggccgcgg ctctcctccg cctgaccgat
     5401 cttgcggtag atcctcgcgc gggcgagcgg attcccgccg ccgcgggcca actgggccaa
     5461 cgcggctcga agggtctcga tcgctcgacc cgcgtccccc gcggcggcca actgatcgcc
     5521 gagcagttcg gtcaggctcg ggtcgtcggg cgcgaggcgc tgcgcccgtt cggcatcggc
     5581 gaccgcggcc aggtgctggc cgagcgccgc gttcgcccgc gaccgctgcc gtagccgatt
     5641 ggcctcgagc accggcgacg cgaacgcatc gagccgatcg acgcccg
//
LOCUS       DASPGN010000387         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_655454, whole genome shotgun sequence.
ACCESSION   DASPGN010000387 DASPGN010000000
VERSION     DASPGN010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655454"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="VD788_11135"
     CDS             <1..1287
                     /locus_tag="VD788_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76857.1"
                     /translation="GAPSAFTVQLPGAQPRAIPETLAIPVALSADATRVGALASCVDG
                     VAPEPLVRTLSFSNPTSGRDKLGAGPNVKLRLTLSCREAEVVFCTAGQGCFGQPGGQL
                     GGDLAMCNDPLAGWLTLHASQVLPLTIGGSHELNDLSTEAGSCSDSDYSAPGANGQGS
                     GFEFQTVCDRSGAIAYLPAGTTPGLLRPDRGSNREISGVADMMATDRQGSGSRGSGAG
                     VLAGQALALKLGLALGETGSAPYVPAGAGSCVENAGEFVCDESAVNAGAGCETAGAAD
                     DRLCFARSGITPAALGDFLLPTELCTQVAGDDRRLAETGCGDDSDDLCRSFVLPDCVT
                     DLSVSELLAAGEEVLSGGASGVCGDDPSALTEALELINRAFDGCGKVIDCSQLPETTD
                     TTRELDRRGGGSNTEESDVSSTGLRTFAEGGTGARR"
     gene            complement(1321..1833)
                     /locus_tag="VD788_11140"
     CDS             complement(1321..1833)
                     /locus_tag="VD788_11140"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV76858.1"
                     /translation="MPDEPLEERDGWYVVNVKDAHWFESPVFGKSVLFEDRDSYRARA
                     VGVRLHILEPGKPNCRYHRESAQENFLVLSGRCRLLVNGEQRRLEPWDFVHCAPGVSH
                     VFVGEGDGPCVMLMVGDRPEHLRIFYPRHAAARAVNAEAPEPTADPRVAYSDVTPWAP
                     TESRDWPLDG"
     gene            2198..3121
                     /locus_tag="VD788_11145"
     CDS             2198..3121
                     /locus_tag="VD788_11145"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HXV76859.1"
                     /translation="MTVLDAIGGTRLLPLRRIVPAGSARVLVKLEYENPTGSMKDRMA
                     LAMIEAAEADGRLGPGGSVVEYTGGSTGVSLAFVCSAKGIPLEIVTSDAFSKEKRDHM
                     TALGARLTLVPSPTGGTTKALTLEMIETARRLAAKPGCFWTDQLNNADPLPSYERMAD
                     ELWRQTGGEIDAFVQSVGTAGSLRGISTRLRALDGAVRVVAVEPDESAVLSGRPSGSH
                     KIEGTGPGFVMPLWTGTLADEIETVSSAEAMAVARRLATEEGVFAGTSTGGNVAVALR
                     VAERLGPGRTVVTIAVDSGMKYLSTPLYSQA"
     gene            complement(3141..>3322)
                     /locus_tag="VD788_11150"
     CDS             complement(3141..>3322)
                     /locus_tag="VD788_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76860.1"
                     /translation="PGDVGCFDDLGDGSVRTALAPGGGSRYYLVTGYRGADQGPAGND
                     GAGVARDPAQQTCTP"
BASE COUNT          477 a         1187 c         1185 g          473 t
ORIGIN      
        1 ggggcgccga gcgcgttcac ggtccagcta cccggcgcgc agccgcgggc gatccccgaa
       61 acgctcgcga tccccgtcgc cctgtccgcg gacgcgaccc gcgtcggcgc gctcgcgagt
      121 tgtgtcgacg gcgtggcgcc cgagccgctg gtccgcacgc tctccttttc gaacccgaca
      181 tccggccgtg acaagctggg cgccggcccc aacgtgaagc tccgcctgac gctcagctgc
      241 cgcgaggccg aggtcgtgtt ttgcacggcg ggccagggct gcttcgggca gcccggcgga
      301 cagctcggcg gtgacctcgc gatgtgcaac gacccgctcg ccggctggct cacgctccac
      361 gcgtcccagg tgctcccgct gacgatcggc ggatcgcacg agctcaacga tctgtcgacc
      421 gaggccggta gctgctccga ctccgactac tccgctcccg gcgcgaacgg ccagggctcc
      481 ggtttcgagt tccagacggt ctgcgaccgg agcggcgcga tcgcctatct tccggcgggc
      541 accacgccgg gccttctccg gcccgatcgt ggctcgaacc gcgagatctc cggcgtggcc
      601 gacatgatgg cgacggaccg ccaggggagc ggctcgcgcg gatcgggcgc cggcgtgctc
      661 gccggtcagg ctctcgcact caagctcggc ctcgcgctcg gcgagaccgg cagcgcaccc
      721 tacgttccgg ccggagccgg ctcctgcgtc gagaacgcgg gcgagttcgt ttgtgacgag
      781 tccgcggtga acgcgggcgc cggttgcgag accgccggcg cagcggacga ccgtttgtgc
      841 ttcgctcggt ccggcatcac gcccgccgcg ctcggcgact tcctgctccc gaccgagctg
      901 tgcacgcagg tcgcggggga cgatcgtcgc ctcgcggaga ccggttgcgg cgacgactcc
      961 gacgacctgt gccggtcgtt cgtccttccc gactgcgtga ccgacctcag cgtgagcgaa
     1021 ctgctcgcgg ccggcgagga ggtcctcagc ggcggcgcga gcggcgtgtg cggggatgat
     1081 ccgtccgcgc tgaccgaggc gctcgaactg atcaaccgcg ccttcgacgg ctgcggcaag
     1141 gtgatcgact gctcgcagct tccggagacg accgacacga cccgcgagct cgaccgtcgc
     1201 ggcggcggat cgaacaccga ggaaagcgac gtgtcgtcga ccggcctgcg gaccttcgcc
     1261 gaaggcggca cgggagcacg acgctgagcg gtcgagtcgg gcgcgagagg cgacccgggt
     1321 tcaaccgtcg agcggccagt cccgcgactc ggtcggggcc cagggcgtga catcggagta
     1381 ggcgactcgg gggtcggcgg tcggctccgg cgcttcggcg ttgaccgcgc gcgccgcggc
     1441 gtgccgcggg tagaagatcc gcaggtgctc cggccgatcg ccgaccatca gcatcacgca
     1501 cgggccgtcc ccctcgccga cgaagacgtg cgagaccccg ggcgcgcagt ggacgaagtc
     1561 ccacggctcg agccggcgct gttcgccgtt gacgagcaag cggcaacgcc cggacaacac
     1621 gaggaagttc tcctgggcgc tctcgcgatg gtagcggcag ttgggtttgc ccggctcgag
     1681 gatgtgcagc ctgacgccga ccgcgcgtgc ccgataggaa tcgcgatcct cgaacagcac
     1741 cgacttgccg aagaccgggc tctcgaacca gtgcgcgtcc ttcacgttca cgacgtacca
     1801 gccgtcgcgt tcctcgagcg gttcgtccgg catcgacttc ctccgcgaaa cccgtcggtc
     1861 gcgtcagcgc gactggccgg cggcgcgcac cgcatcgacg acttcctcga cctcgggcca
     1921 cggccggcgg ctgagcctgg ccagcagccc gccccgccgc gaaacgacgg tgcggccgtc
     1981 gacgacgacc tcgaactggc cgcgtcggcc cgcctcgagc ccgacgtcga caccgagggc
     2041 gtcctcgatc cgggcggcga aactcgccgc cttctccgcc gtggtgactc aggtccggca
     2101 gtggacgatg cgcacgttca tcgccggcga gtataacagc tcgaccgtcg cgtgctccgc
     2161 gccgctgtat tatcgtcgga gcgatgagga gggccggatg acggtgctcg acgcgatcgg
     2221 cgggacgcgg ctcctgccgt tgcgacggat cgtgccggcg ggcagcgcgc gcgttctcgt
     2281 caagctcgag tacgagaacc cgaccggcag catgaaggat cgcatggcgc tcgcgatgat
     2341 cgaggcggcc gaggcggacg gtcgactcgg gcccggcggc tcggtcgtcg aatacaccgg
     2401 cggcagcacc ggggtgtcgc tcgccttcgt ctgctccgcg aaaggcattc cgctcgagat
     2461 cgtgacctcg gacgcattca gcaaggagaa gcgcgatcac atgaccgcgc tcggcgcacg
     2521 cctcacgctc gtgccgagcc cgaccggcgg cacgaccaag gcgctgacgc tcgagatgat
     2581 cgagaccgcg cgccggctcg cggcgaaacc gggctgcttc tggaccgacc aattgaacaa
     2641 cgcggatccg ctgccgagct acgagcggat ggcggacgag ttgtggcggc agaccggggg
     2701 cgagatcgac gccttcgtgc agtccgtcgg cacggccggt tcgttgcgcg ggatctcgac
     2761 gcgtctccgc gcgctcgacg gcgcggtgcg ggtcgtcgcc gtcgagcccg acgagtcggc
     2821 cgtgctgtcc gggcgcccca gcggttcgca caagatcgag ggtaccggtc ccgggttcgt
     2881 catgccgctg tggaccggca cgctcgccga cgagatcgag acggtgtcgt cggcggaagc
     2941 gatggcggtg gctcgacgcc tcgccaccga agaaggcgtc ttcgccggaa cgtcgaccgg
     3001 cggcaacgtc gcagtcgcgt tgcgcgtcgc cgagcggctg ggtcccggcc gcaccgtcgt
     3061 cacgatcgcc gtcgactccg gtatgaagta cctcagcacg ccgctgtact cgcaggcgtg
     3121 aggaccgacc tcccgggccc tcaaggcgtg cacgtctgct gcgccggatc gcgcgcaacg
     3181 ccggccccgt cgttgcccgc gggaccctgg tcggcgcctc gatacccggt gaccaggtag
     3241 taacgggatc cgccgccggg cgccagggcc gtgcggaccg agccgtcgcc gaggtcgtcg
     3301 aagcagccga cgtcgccggg ga
//
LOCUS       DASPGN010000388         5235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_772742, whole genome shotgun sequence.
ACCESSION   DASPGN010000388 DASPGN010000000
VERSION     DASPGN010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_772742"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1712
                     /locus_tag="VD788_11155"
     CDS             <1..1712
                     /locus_tag="VD788_11155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV76861.1"
                     /translation="SARGIPCSMTLSGSSTEIERQVRWLMTLRGVTVTTLLVCAFGVE
                     LLLRPIETLRPLFTLAAVAYGMVLLYAVLYRWLSGTRFFVVLQLVGDAVIVTFFVGIT
                     GGIDSPMSFLYLLPIAVASLLLYRSGGLALAAVCWGFYAASVLGLDVSPVSGSLSREP
                     AHVVYVLVAHLVGMLACAVLSSHLAERMRAQGRELAERRGAVARLKALNENIIESINS
                     GLVTTDLSGRITFINRGGTEILRDAAERFDGRSIESVFRLEPGFLDEIREKLTANLRF
                     RFEQHHQTPDGATIFLGIAASNLHDRQGNPLGYIFIFQDLTEIHALEQEMRLKERMVA
                     LGEMAAGMAHELRNPLAAISGSVQYLKGDLRPSGETLELMDIILRESQRLDQAIKDFL
                     TFARPGTFAPETVDLVRLIEDHVKLLKKSREFTPSHAIETDYPTATVVCEADPNRLKQ
                     VCWNLAINALKAMPDGGKLTIRVALADGGRRLDISFSDEGQGMTEEDRERYFQPFSGS
                     FRQGTGLGAAIVYRLVEEHRGRIVLDSAAGRGTCVTITLPRYTITRTEQAAPKPQLRA
                     AGG"
     gene            1716..3197
                     /locus_tag="VD788_11160"
     CDS             1716..3197
                     /locus_tag="VD788_11160"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV76862.1"
                     /translation="MSKILIVEDEKSMRDLLALMLRKEGYEVETVESAELGRDWLAAK
                     GPTDLVISDISMPGISGLELLRFSRKTCPETAVILMTAYGTKQTAIEALNEGASYYVE
                     KPFDLDEMKVVVRKTLEHRRIARENRDLRDQNRGLRAELTEKYCFDGLVGRSAKMRAI
                     FELIERVAATNSTIMISGESGTGKELIARAVHYNSGRRDNPFVSINCGALPDDLLESE
                     LFGHMKGSFTGATANKKGLFEVANGGTIFLDEIGETSAAMQIKLLRVLQERTIRRVGG
                     TEEIAVDVRVITATNQDLESMVQERTFREDLYYRINVIAIRMPALREKPEDIPALAEY
                     FLDKYRAVVGKPIPGGISKAALEALETYAWPGNVRQLENVIERAVALEASEQIQPESL
                     PVEVRSGARAGGELDVVLPESGIDLEQHLEDLRQRYMLEAMERSGGVQTKAAEMLGMT
                     FRSFRYFAKKYELTTGRDGGGADSGEERHPEEAASRIIAGGQS"
     gene            3403..3852
                     /locus_tag="VD788_11165"
     CDS             3403..3852
                     /locus_tag="VD788_11165"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXV76863.1"
                     /translation="MMRRREKGFTLIELLIVVAIIGIIAAIAIPNLLNAIDRGKQKRT
                     MADLRSVGTAIESYSIDNNFYPNAGTTLGTLQSLIEPLYIRTAPSNDGWNRAFNISCS
                     TTEYTVCSGGKDGGNCVNDANGATQTFNDSITFSNGQFVQWPEGAQQ"
     gene            3970..4416
                     /locus_tag="VD788_11170"
     CDS             3970..4416
                     /locus_tag="VD788_11170"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXV76864.1"
                     /translation="MRSRRNSEAGFTLIELLVVVAVIGVVAAISIISYFTALDKSRQR
                     ATMADMRSVARALEAYMVDHNFAPDSTGGVASLTNVLIPYQINVVPVRDHWGNLYNFE
                     SNAFDNYTLESFGKDATPGADISYATRMDFNLDILISDGVFKAAPE"
     gene            4413..>5235
                     /locus_tag="VD788_11175"
     CDS             4413..>5235
                     /locus_tag="VD788_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76865.1"
                     /translation="MKPTLPPDDRPVSSDGSRLLPPTLAIVLVLLPPETRGESAAGCA
                     ALVLLLVMLAARRTLPAWVVWLPIAGTAAVVPELWLGQAPGAVVEPLAHRVLAASAGL
                     AVATIGIPTEERSRFPLALAVAAAVAATFGLYQVVWGFERIVEGIRQGGVVADAARVV
                     ARTEAGRAFAAFPTPAALGGLLILSLPITIGAALARRGAARAAVVVLAALQAAGLLAT
                     ASVTAVGGLAAALVVWGVRRAGRSSKRAVVAGLAATAAVAVVVVMRGPAVTDLGAP"
BASE COUNT          878 a         1660 c         1821 g          876 t
ORIGIN      
        1 ggtccgcccg cgggattccg tgttcgatga cgctgtccgg ctccagcacc gagatcgaac
       61 ggcaggtccg ctggctgatg acgctgcgcg gcgtcacggt cacgacgttg ctggtctgcg
      121 ccttcggcgt cgagctgctg ctccggccga tcgagacgct gagacccctg ttcaccttgg
      181 ccgccgtggc ttacggcatg gtgctgctgt acgccgtgct ctaccggtgg ctgagcggca
      241 cacggttctt cgtcgtgctg cagctggtcg gcgacgcggt gatcgtgacc ttcttcgtcg
      301 ggatcacggg cgggatcgac agcccgatgt cgttcctcta cttgctgccg atcgccgtcg
      361 cgtcgctgtt gctctatcgc agcggcgggc tggcgctggc cgcggtttgc tgggggttct
      421 acgcggcctc ggtgctgggg ctcgacgtgt cgccggtctc cggcagcttg tcgcgcgagc
      481 cggcgcacgt ggtctacgtg ctggtcgcgc atctggtcgg catgctggcc tgtgccgtgc
      541 tgtcgtccca cctcgccgag cggatgcggg ctcaggggcg cgagctggcc gagcgccgcg
      601 gggcggtcgc ccggctgaag gcgctcaacg agaacatcat cgagtcgatc aacagcggac
      661 tcgtcacgac cgacctgagc ggtcggatca ccttcatcaa ccgcggcgga accgagatct
      721 tgcgcgacgc cgccgagcgc ttcgacggga ggtcgatcga gtcggtcttt cgcctcgagc
      781 cgggctttct cgacgagatc cgcgagaaac tgaccgccaa cctgcgcttc cgcttcgagc
      841 agcaccacca aacaccggac ggggcgacga tcttcctcgg catcgcggcg tccaacctgc
      901 acgatcgcca gggcaacccg ctgggctaca tcttcatctt ccaggacctg accgagatcc
      961 acgcgctcga gcaagagatg cggctgaagg agcggatggt cgccttgggt gaaatggcgg
     1021 ccggcatggc ccacgagctg cgcaacccgc tggcggcgat cagcggctcg gtccagtatc
     1081 tcaagggtga cctgcgcccg tcaggcgaga cgctcgagct gatggacatc atcctgcgcg
     1141 agtcgcagcg gttggatcag gcgatcaagg actttctgac cttcgcccgc ccggggacgt
     1201 tcgcccccga aaccgtcgat ctggttcgac tgatcgagga tcacgtcaag ctactgaaga
     1261 agagtcgcga gttcaccccg agccacgcga tcgagaccga ctacccgacg gcgacggtcg
     1321 tctgcgaagc cgacccgaac cggctgaagc aggtctgctg gaatctggcg atcaacgcgt
     1381 tgaaggcgat gcccgacggc ggcaagctga cgatccgcgt ggcgctggcc gacgggggtc
     1441 gccggctgga catctccttt tccgacgagg ggcagggaat gaccgaggaa gaccgcgagc
     1501 gctacttcca accgttcagc ggctcgttcc gtcaggggac gggtctcggc gcggcgatcg
     1561 tctaccgctt ggtcgaggaa caccgcggcc ggatcgtgct cgattccgca gcgggacgcg
     1621 gtacctgcgt gacgattacc ctgccccgct acacgatcac ccgcaccgag caagcggcgc
     1681 ccaagccgca gttgcgtgca gccggaggtt gagcgttgag caagattctg atcgtcgaag
     1741 acgagaagag catgcgcgac ctgctcgcgt tgatgctgcg caaggaaggc tatgaggtcg
     1801 agaccgtcga gtcggccgag ctcggccgcg attggctcgc ggccaagggg ccgacggacc
     1861 tggtcatcag cgacatctcg atgccgggaa tctccgggct cgagctgctg cgcttcagcc
     1921 gcaagacctg tcccgagacg gcggtgatcc tgatgaccgc ctacgggacg aagcagaccg
     1981 cgatcgaggc attgaacgag ggcgcgagct actacgtcga aaagccgttc gacctggacg
     2041 agatgaaggt cgtcgtgcgc aagacgctcg agcacaggcg gatcgcgcgc gagaaccgcg
     2101 atctgcgcga ccagaaccgc gggttgcgtg ccgagttgac cgagaagtac tgcttcgacg
     2161 gcttggtcgg gcgcagtgcg aagatgcggg cgatcttcga gctgatcgag cgcgtggcag
     2221 cgaccaacag cacgatcatg atctcgggcg agtccggcac cggcaaggag ctgatcgcgc
     2281 gggcggtgca ctacaactcg ggccggcgcg acaatccgtt cgtatcgatc aactgcggcg
     2341 cgctgcccga cgacctgctc gagagcgagc tgttcggcca catgaaggga tcgttcaccg
     2401 gcgcgaccgc caacaagaag ggtctgttcg aggtggccaa cggcgggacg atcttcctcg
     2461 acgagatcgg cgagacgtcg gccgcgatgc agatcaaact gttgcgcgtg ctgcaggagc
     2521 gcacgatccg ccgggtcggc ggcaccgagg agatcgccgt cgacgtccgg gtgatcacgg
     2581 cgaccaacca ggatctcgag tcgatggtgc aggagcggac gtttcgcgag gatctgtact
     2641 accggatcaa cgtgatcgcg atccgcatgc ctgcgttgcg cgagaaaccg gaggacatcc
     2701 cggcgctggc cgaatacttc ctcgacaagt atcgcgcggt ggtgggcaag ccgatccccg
     2761 gaggaatctc gaaggccgcg ctcgaggcgc tcgagaccta cgcgtggccg gggaacgtgc
     2821 gccagctcga gaacgtcatc gagcgggcgg tggcgctcga ggcgagcgag cagatccaac
     2881 cggagagtct gccggtcgag gtgcgcagcg gggctcgcgc cggcggcgag ctggacgtgg
     2941 tgctacccga gagtggcatt gatctggagc agcacctcga agatcttcgt cagcgctata
     3001 tgctcgaagc gatggagcgc agcggtggcg tccagacgaa ggcagccgag atgctcggga
     3061 tgaccttccg ttcgttccgc tacttcgcga agaagtacga gttgacgacg gggcgcgacg
     3121 gcggaggcgc cgactcgggt gaggagcgtc acccggagga agccgcatcg cgcatcattg
     3181 ccgggggcca gtcgtgacag gatttgtcac cgggcgtagg acccgagcga cagcccgggg
     3241 cagcatccgg cgctccgacg gccgatcggg aggtggcggc cgcgggcgat gtgtggtcaa
     3301 gttgttgtca gacaatgatt tgtcagcgga gtgcagctga acccgcaagt tggcatccct
     3361 gttgctgtat cggcgggtgg accgacaaag gaggcctcga acatgatgcg tcgtagagag
     3421 aagggtttca ccctgatcga actgctgatc gtggtcgcga tcatcggcat catcgccgcg
     3481 atcgcgatcc ccaacctgct caacgcgatc gaccgtggca agcagaagcg aaccatggcc
     3541 gacttgcgtt cggtcggtac ggcgatcgag tcgtactcga tcgacaacaa cttctacccc
     3601 aatgcgggga cgactctcgg cacgttgcag agtctgatcg agccgctgta catccgtacc
     3661 gcgccgtcga acgacggatg gaaccgcgcc ttcaacatct cgtgctcgac gaccgagtac
     3721 accgtctgct ccggtggtaa ggacggcgga aactgcgtca acgacgcgaa cggtgcgacg
     3781 cagacgttca acgactcgat caccttctcc aacggccagt tcgtgcagtg gcccgagggc
     3841 gctcagcagt aagctcgtca ggactgacgg tcgagagaga caaatggggg cccgcccggc
     3901 ccccatttgt tttctcgctc cgatcatcca tccgcaatcg atcgctcgga gggcgaggga
     3961 catcccggaa tgagaagccg acggaacagc gaagcgggct tcacgctgat cgagctgctg
     4021 gtggtggtgg cggtgatcgg ggtggtggcc gcgatctcga tcatttcgta cttcaccgcg
     4081 ctcgacaagt cgaggcaacg cgcgacgatg gccgacatgc gcagcgtggc gcgcgccctc
     4141 gaggcctaca tggtcgatca caacttcgcg ccggattcga ccggcggcgt ggcgtcgctg
     4201 accaacgtgc tgattccgta ccagatcaac gtggtgccgg tccgcgatca ctggggcaat
     4261 ctctacaact tcgagtcgaa tgccttcgac aactacacgc tggaatcgtt cggcaaggac
     4321 gcgactcccg gggccgacat cagctatgcc accaggatgg acttcaatct tgacatcctg
     4381 atcagcgatg gagtattcaa agcagctccg gagtgaaacc aaccctacca cccgacgacc
     4441 gtcccgtatc gagcgacggt tcccgcctgc tcccgcccac acttgccatc gtcctggttc
     4501 tgctgccgcc ggaaacccgc ggcgagtcgg ccgccgggtg cgccgcgctg gtcctgctgc
     4561 tcgtgatgct cgccgcgcgt cggacgctgc cggcgtgggt cgtttggctg ccgatcgcgg
     4621 gcaccgcggc ggtcgtgccc gagctgtggc tgggccaggc gcccggcgcc gtcgtcgagc
     4681 cgctggccca tcgggtgctg gcggcatcgg cgggtctggc cgtcgcgacg atcgggatcc
     4741 cgaccgagga acgatcgcga ttcccgctcg ccctcgccgt cgccgccgcg gtcgccgcga
     4801 cgttcggtct gtaccaggtc gtgtggggct tcgagcggat cgtcgagggg attcgtcaag
     4861 ggggcgtcgt ggccgatgcg gcacgggtcg tggcccgcac cgaggcgggg cgcgcgttcg
     4921 ccgccttccc gacaccggcc gcgctcggtg ggttgttgat cctgagcctg ccgatcacga
     4981 tcggcgcggc gctggcgcgg cgcggcgcgg cgcgcgcggc cgtcgtcgtg ctggccgcgc
     5041 tgcaggcggc ggggctgctg gcgaccgcgt cggtgaccgc cgtcggcggg ctggcggccg
     5101 cgctcgtcgt ctggggggtt cgtcgcgcgg gccggagctc gaaacgcgcc gtcgtcgccg
     5161 gcttggccgc gacggccgcg gtggcggtcg tcgtcgtgat gcgaggcccg gcggtgaccg
     5221 acctcggcgc gccgg
//
LOCUS       DASPGN010000389        12570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_70122, whole genome shotgun sequence.
ACCESSION   DASPGN010000389 DASPGN010000000
VERSION     DASPGN010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12570
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70122"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VD788_11180"
     CDS             complement(<1..187)
                     /locus_tag="VD788_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76866.1"
                     /translation="MNRVVLRLAAIALFPLAVAAAEAPARPEVQHEIDQLVRAITTTE
                     RAGDEAAFVARADRLQQI"
     gene            complement(184..1671)
                     /locus_tag="VD788_11185"
     CDS             complement(184..1671)
                     /locus_tag="VD788_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76867.1"
                     /translation="MTARSWIRTLVPLAFLSGGYATTHGAPGEPTAVTVVDRDPATGE
                     LSIRYEPACDAADHHIEYGRIEDLGPVLVYSGQVCGIGTTGAFDGVIPESDSFFFLVV
                     GNDGVALEGSYGTSLIAGSFEERADDTLDPGCALVQQLVDRCDLPTVELTAYRPQSEA
                     YGAPLMRRAVPEAELLSPGVGIRVNGDDDDDDGTPDLDDGSVIGENDLIELTLAASLP
                     LAPAGLEYALIRTSGDVRVWSDGAKGTEILGTADEAIVSFAGGPLTTWLEQPTAGSSS
                     LTLVARRTSEMSIVASAELSVFTFSSIVIALGGEDQVPGDPPLVPGNHGTFEVAIELY
                     RRGYDVHMYDEDVVSASGAGLAFDEVVSAVRDRGVGRIAIYGYSHGAGSTNDLARRLD
                     DNRASIGTFSIDFTGYVDAIDNDSDIDIGTETELPPSTGYHANYYENPGCGLFVLCGG
                     PIAGADFDLDVNTTPWGAELDHFTVDDAPEVIQGLLDQLVIQVAP"
     gene            complement(2042..2809)
                     /locus_tag="VD788_11190"
     CDS             complement(2042..2809)
                     /locus_tag="VD788_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76868.1"
                     /translation="MSRLSKIPIALALLCPILAFTAGCSDDEDETSGGVSAQQAAAFL
                     EQVVPDVIEFSDGLAALIRGATTVARATGGVPTCQPIEIDGTPLCLSGVGESCPGTGG
                     EFQWAFAQCNSALLEVTLDGTIDFSGAAPSFDVVFTDLSILSTSGTQTIDGGIDVLLE
                     ITGCNTVSYQGLELFYDGISIVLPSGGTLTICGINNTTGFFTALIDATGVAAFEADVS
                     LFGSTGSALVSQGGVPLYDCSLTIGGAASCTDVEPPG"
     gene            2942..3586
                     /locus_tag="VD788_11195"
     CDS             2942..3586
                     /locus_tag="VD788_11195"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXV76869.1"
                     /translation="MPQQPTSETQPTRERLLDAAARLFHERGFEATPVAAILERAGVH
                     SGSLYHYFPGKDALLRGVLERYRGLLQPVVLGPIEEAERDPIERVFRLLDWYRQGMTT
                     SRCRRGCPIGNLALEVSDTHPEVRPIIAQNLAAWAAGIERWLDEAADRLPADCDRQAL
                     AGFVLTVMEGGLLEARALGDLAPFDRSVAVLRDHVNRLLADAAGRPAGAQEDRR"
     gene            3583..4167
                     /locus_tag="VD788_11200"
     CDS             3583..4167
                     /locus_tag="VD788_11200"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXV76870.1"
                     /translation="MNRRRFAVPSLIPVAVAVAVAVAGVWAAPAPTADPIDSTLFVAG
                     GDAVRSFTKEIVIDAPVGRVYDAWTTSEGWSALYGAPSVSHIDLAIGGRYEWLFDGRI
                     GSNGCQVLSYVPDRMVSFSWNAPPSQSSSRVRRTWVVVETFEEGPATTRVRLTHLGFG
                     DGAEWDETLRYFDEAWDRVLGRLETAFASERAAD"
     gene            complement(4225..4710)
                     /gene="pgaD"
                     /locus_tag="VD788_11205"
     CDS             complement(4225..4710)
                     /gene="pgaD"
                     /locus_tag="VD788_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HXV76871.1"
                     /translation="MNKPGFDDRTIDARDRLTQKIRRRDGVLTIVLWVVYAYLWLPLV
                     SLFAWYLGFSFAYRLVEQAGGPSRLVRLLAWFALFAVVAAAGVVTWSRLELRRFGVRE
                     RRRSSPVLTPQQEIDHWQIEQGRFDQIRNSQRLLIDLDARGRFLSVADRSQRGLLVPR
                     D"
     gene            complement(4691..5974)
                     /gene="pgaC"
                     /locus_tag="VD788_11210"
     CDS             complement(4691..5974)
                     /gene="pgaC"
                     /locus_tag="VD788_11210"
                     /inference="COORDINATES: protein motif:HMM:TIGR03937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HXV76872.1"
                     /translation="MSWYALVVALFDFTFFYPLIMSILWVTGSVYYFFYRERASPAHP
                     DAPPDLAQWPPVSFLVPCHNEGTHVVETIQSLLDQDYPVFEIIAVNDASTDETGERLD
                     RLSREDYRVRVIHFRTNQGKAMGLRVASLAAKHEILVAVDGDALLTPRATKWLARHFV
                     DGPRVGAVTGNPRVRNRSTLLGKIQVGEFSSIIGLLKRAQRIYGRVFTVSGVVAAFRK
                     AAVHQVGYWGLEVMTEDIDVSWRLQLAHWDIRYEPNALCWILMPETLRGLWRQRLRWA
                     QGGMEVLGGHFPSLFRWRARRMWLVAAELVASTFWAYAMAAVFATWVAGRFVDLPAPF
                     AGATVFPGWSGLLLGTMCLLQFLVSVVIDSRYESRLGRVYYWLIWYPMVYWAIQVVTS
                     VYALPRALLRPKWSRGTWTSPDRGLARRVYEQTRV"
     gene            complement(5971..7893)
                     /gene="pgaB"
                     /locus_tag="VD788_11215"
     CDS             complement(5971..7893)
                     /gene="pgaB"
                     /locus_tag="VD788_11215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HXV76873.1"
                     /translation="MTRAAIAFLALSITAAGAADVELVTLTYHDVRSHLVGNHDPDRY
                     AVSSANLAAHFRWLASQGYHVVSVERIAAALAGGEPLPPRAVLLTFDDGFRSAYTHVF
                     PLLETFGYPAVLSLVTSWIEAEGAIDYDGVALGPEDFLSWAQIREMQASGLVEIASHS
                     HDLHRGIRANPQGNLEPAAVALAWDDEGYESEANYRERVAADLSTSARAIEDHTGRRP
                     RVIAWPFGAWNRPIREIAAGLGMELSLTLQEVEFASRDGVFGREMLVSNPGIPRFSAM
                     FAPRQARPLRAAQVDLDYVYDADPERQEINLGLLLDRIEQLEISHVFLQAFADPDGDG
                     AADALYFPNRRLPVRADLFNRAAWQLRTRAHVRVFAWLPLLAYRGEAVDPDWRVLQQA
                     GDTRQPDPAGETRLSPFHPDARRFIADIYRDLALHAQLDGIHFHDDGRLNEFEDVSPA
                     ALGAYERETGEPFSFDGVDRDADRTRRWAAIKSAALIEFSRELVEIVRVWHPGSLTSR
                     NLFAPAVVDPEAEIYLAQDYADYLANYDYTTLMAMPRLEDTERERRFYRRLIQAVGGV
                     AAGLDKTVFQLQTVDWRSGRPIPSDELRDTLRFLQSYGVRHLAYYPDDLVAGHPRVDL
                     LRQGLSLADMPRSGDR"
     gene            complement(7890..10283)
                     /gene="pgaA"
                     /locus_tag="VD788_11220"
     CDS             complement(7890..10283)
                     /gene="pgaA"
                     /locus_tag="VD788_11220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HXV76874.1"
                     /translation="MLTYPPTTRGDDPQAERGSLRERREEAVELARSGQYERALESFE
                     RLRAEYPEEPSVLYDQTVVLFWAGRHTDALGNAPWIDPRLAPAYVISAVAGSARNTQQ
                     FAMAVEWYGHALAADPDSVDARVGLAMSLADAGEPRRARQTLEAVEPAARQTARVRLS
                     SAYLYTRDGAQLEAIGEYDAVLEVDPDNFEALRGKAQALRALLLPRQALELARQHPGI
                     LDEREIARTEGDALALDLRDAVESAGEGPPYARIDEAIAAIDRRLATEPSTGELARRL
                     RYDRIVGLVAAGRPQPAVADYESLIAEGAEPPTYALIAAGSAYLALRRPREAERALRR
                     GEAIDPGNVALRIELFYALIALERFGEAFRVIDGLSAELEPAPRAPGSPVGQPNPVQL
                     DVEIIAAVGRAYADQLAEAQRRLEHLLARAPNNAQARYELGNVYRWRGFHRRAERQYV
                     QVGDHGGVGDVWRDVAAAHNDLDLGRPASTGRTLSRLGVEQPATLAVRDLERRWAAHN
                     RSRLSIDARYGNSSGEVFGSEQYQIDGWWFSPPLGHEYRIYVRTFDSWARFDDGETAR
                     RRLAAGVEYGRGGLRAAGEINGDRFDFDSPGAAARLDWSITDHWLFGCVAEYDSYATQ
                     LRADRAGIDSNLFSARGTYRGSESWYVSLALGHQPYDDGNRMTAVAADGRLRLYNGYK
                     YKVDGFALVESSTGSDADVPYFSPERALVARIGIANDWRQWRVYDRSLTHRLSADAGL
                     YDQRGFGADPIWTLDYRLQWNLNARWQLWFGAQRNRRVYDGSREDATFWTFGVDGRP"
     gene            10510..11037
                     /locus_tag="VD788_11225"
     CDS             10510..11037
                     /locus_tag="VD788_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76875.1"
                     /translation="MLGRLVVGLLLLSVASRAPAAEADAPRQDPWKGKTRSEVVGLLG
                     EPDKTKTDKQGVLSLTYRFNRIDPMAPSQPDAIFLQVPGVGVVARIDKSRRPPPDAMT
                     IEPPQFDEQGRQTAGGVSETRSATTSYDIDSKEVSHSTSASAGPPGGSRVTLRFEIGS
                     DGRVVDWTTTGEKGR"
     gene            11063..>12570
                     /locus_tag="VD788_11230"
     CDS             11063..>12570
                     /locus_tag="VD788_11230"
                     /inference="COORDINATES: protein motif:HMM:NF028457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5117 domain-containing protein"
                     /protein_id="HXV76876.1"
                     /translation="MNRRPPGCRRATRVALPLLVVLASVAGLPAKKREPTTPGEPTLA
                     ALTEGAETIDGLLTFYRTEDKLYLRLPAELVGAPLGFAAVRVHAGGDYAMRGGAIDNQ
                     LVRWRVVGDRLVLVKENLDFRAEHDPTTERLLESSFSDSPVFSAPLVRTNDEPAPLVI
                     DASKLFGPDLAQLLPDDAGYDVRADDAILASLESFDDNVVARVVYRAHRQAKPAEQAA
                     NPFSRFVRPGRLPDARAAEITVDYNFYRLEDDGFRPRAADERIGGMTLSYKDYSDVDN
                     ADTLFRHVLRRFDLRKVDPSAAVSDPIEPITFYIDRSVPERWRPLVREATLWWNPAFE
                     AVGLRNALRVLDAPDDPRWDPADIRHSVIFWNYSDDLVFSGVAGPIFVDPRNGKTIKA
                     TVHLNGEFFSYGLHRYLVYAWWRAPAPGADATTWQARRDAARALRRAPAACDRAASFS
                     SQMAFARLVLRSRGVIGADPAEDDRFIREAFRELVAHEVGHALGFPHNWKASL"
BASE COUNT         1955 a         4368 c         4405 g         1842 t
ORIGIN      
        1 cgatctgttg cagccggtcg gcgcgcgcga cgaacgcggc ctcgtcgccg gcacgctccg
       61 tcgtggtgat cgcccggacc agctggtcga tctcgtgctg gacctccggg cgagcgggcg
      121 cctccgccgc ggccacggcg agcggaaaca gggcgatcgc cgccagccga aggacgaccc
      181 ggttcacggc gccacctgta tgaccagctg gtccagcagt ccttggatga cctcgggcgc
      241 gtcgtcgacg gtgaagtgat cgagctcggc accccacggc gtcgtgttga cgtcgagatc
      301 gaagtcggca cccgcgatcg gtcccccgca gagcacgaac agaccgcagc ccgggttctc
      361 gtagtagttg gcgtggtagc cggtggaagg cggcagctcg gtttccgtgc cgatgtcgat
      421 gtcggagtcg ttgtcgatcg cgtcgacgta gccggtgaag tcgatcgaga aggtcccgat
      481 cgaggcgcgg ttgtcgtcca gacgacgcgc caggtcgttg gtcgaacccg caccgtggct
      541 gtagccgtag atcgcgatgc gacccacccc acggtcccgc accgcgctca ccacctcgtc
      601 gaacgcgagg ccggcgccgc tggcggagac cacgtcctcg tcatacatgt gcacgtcgta
      661 gccgcggcgg tagagctcga tcgcgacctc gaacgtcccg tgattacccg gcacgagcgg
      721 aggatcgccg ggcacctggt cctcgccgcc gagcgcgatg acgatgctgg agaaggtgaa
      781 gaccgacagt tcggccgagg cgacgatgct catctccgac gtccgccgag ccaccagggt
      841 caacgacgac gaccccgccg tgggttgctc gagccaggtg gtcagcgggc cgccggcgaa
      901 cgagacgatc gcctcgtcgg cggtcccgag gatctccgtg cctttggccc cgtcggacca
      961 gacgcgtacg tcgcccgacg tgcgtatcag cgcgtactcg agacccgcgg gcgcgagcgg
     1021 aagtgaggcg gcgagcgtga gctcgatcag gtcgttctcg ccgatgacgc tgccgtcgtc
     1081 caagtcggga gtcccgtcgt cgtcgtcgtc gtctccgttc acgcggatgc cgacacccgg
     1141 ggagagcagc tccgcttccg ggacggcccg gcgcatcagc ggtgccccgt acgcttcgga
     1201 ctgcggccgg taggcggtca gctcgacggt cggcaggtcg caacgatcca cgagctgttg
     1261 aaccagcgcg caacccgggt cgagcgtgtc gtcggcacgt tcttcgaacg atccggcgat
     1321 caacgacgtg ccgtacgagc cctccagcgc gaccccgtcg ttgccgacga cgagaaagaa
     1381 gaacgagtcg ctctcgggga tcacgccgtc gaaggcgccc gtggtgccga tcccgcacac
     1441 ctgacccgaa tagaccagca ccggtcccag gtcttcgatc ctgccgtatt cgatgtggtg
     1501 gtcggccgca tcgcacgccg gctcgtagcg gatcgacagc tcgccggtcg cgggatcgcg
     1561 atcgacgacc gtcaccgcgg tgggctcccc gggtgccccg tgggtcgtgg cgtatcctcc
     1621 cgacaggaag gcgagcggca cgagagtccg gatccagctg cgagcggtca acggttggtt
     1681 ccccggcgag acttcccgtc caaaggtacg gcgaactcgt ccggccgaca gcgggagccg
     1741 cgcgccgtgg tcgaggccgg cccggatcga agaccaaccc gacgcacgat cggctcgcgc
     1801 ggggagcttc cgctcgcacc gtcgacggtc gcgatcggtc gcggccgcgc agcaccgcga
     1861 gaggtcgctc gagctcggcc aggaccgtct tcgccaggtc ctggcgccgg tgagcgaact
     1921 gctcgaggct gtcgtgggtg cccgccgtct cgaaggtgca cgcggcgatc cggctcacgc
     1981 cgatgccgct cgcgccgctc gcgctcgaac gactccgggc cgaggaccgt ctttcggtcg
     2041 atcagcccgg cggctcgacg tcggtacagg aggccgcgcc gccgatcgtg agcgagcagt
     2101 cgtagagcgg tacgccgcct tgcgagacga gcgccgaccc ggtgctcccg aacagcgata
     2161 cgtcggcctc gaacgccgcc acgccggtcg cgtcgatcaa cgcggtgaaa aagccggtcg
     2221 tgttgttgat gccgcagatc gtcagcgtcc cgcccgaagg aagcacgatc gagatcccgt
     2281 cgtagaacaa ctcgagtccc tgataggaca cggtgttgca gccggtgatc tcgagcagga
     2341 cgtcgatccc gccgtcgatc gtctgcgtgc cgctcgtcga caggatcgac aggtcggtga
     2401 agaccacgtc gaagctcggc gcggcgcccg agaagtcgat cgtcccgtcg agcgtgacct
     2461 cgaggagcgc cgagttgcat tgcgcgaagg cccactggaa ctcgccaccg gtccccgggc
     2521 acgactcgcc cactccggac aggcagagcg gggtaccgtc gatctcgatc ggctggcagg
     2581 tcggcacgcc gcccgtcgcc cgcgcgaccg tcgtcgcgcc gcggatcagc gcggccagac
     2641 cgtccgagaa ctcgatcacg tccggcacga cctgctcgag aaacgctgcg gcctgctgtg
     2701 ccgaaacgcc tcccgacgtc tcgtcctcgt cgtccgagca gccggcggtg aacgcgagga
     2761 tcgggcagag caacgcgagg gcaatcggga tcttcgacag gcggctcacg atcggtcctc
     2821 cggaatcccc gcggagccta cgcccttccg cgccgggaaa caagggacac ccggagattc
     2881 gggcttgacg gggagtggtt ctcgcggtat tgattgatcg ttcaatcaag tcaggtgtac
     2941 attgccgcag cagccgacca gcgagaccca gccgacgcgc gagcggttgc tcgacgccgc
     3001 ggcccggctg tttcacgagc gtggcttcga ggccacgccg gtggccgcga tcctcgagcg
     3061 agcgggtgtc cactccggca gtctctatca ctactttccc ggcaaggacg ccctgctgcg
     3121 gggcgtgctc gagcgctacc gcgggttgct gcagccggtc gtgctcgggc cgatcgagga
     3181 ggccgagcgc gatccgatcg agcgtgtttt ccgcctgctc gactggtacc ggcaggggat
     3241 gacgaccagc cggtgcaggc gtggttgccc gatcggcaat ctcgcgctcg aagtgagcga
     3301 cacgcacccc gaggtccggc cgatcatcgc ccagaacctg gcggcgtggg ccgccggcat
     3361 cgagcgctgg ctcgacgagg ccgccgatcg gctgccggcc gactgcgacc gccaggcgct
     3421 cgccggtttc gtcctgacgg tgatggaggg tgggttgctc gaggcgcggg cgctgggcga
     3481 cctcgccccg ttcgaccggt ccgtcgccgt gttgagagat cacgtcaacc gcctgctggc
     3541 cgacgcggcc ggccggccag ccggagccca ggaggaccgt cgatgaaccg acgccgattc
     3601 gccgtcccgt ccctcatccc cgtcgccgtc gccgttgccg tcgccgtcgc gggcgtgtgg
     3661 gccgcacccg cgcccaccgc cgacccgatc gacagcacgc tcttcgtcgc gggtggcgac
     3721 gcggtgcggt cgttcaccaa ggaaatcgtc atcgacgcgc cggtcggtcg cgtctacgac
     3781 gcctggacca cgagcgaggg ttggtcggcg ctctacggag cgccgagcgt cagccacatc
     3841 gacctcgcga tcggcggtcg gtacgagtgg ctgttcgacg gccggatcgg cagcaacggg
     3901 tgccaggtgt tgagctacgt gcccgaccgg atggtcagct tctcgtggaa tgccccgccg
     3961 agccaatcga gctctcgcgt cagacgcacg tgggtcgtgg tcgaaacgtt cgaggaaggc
     4021 ccggcgacga cacgcgtccg gctgacccat ctcggtttcg gcgacggggc cgaatgggac
     4081 gagacgttgc ggtacttcga cgaggcgtgg gaccgcgtgc tggggagact cgagacggca
     4141 ttcgcctccg agcgcgccgc cgactgatgg cggcggcccg tcgcgagcgc gaaggcggct
     4201 cgggggtcag gcggcgggtg agggtcaatc gcgcgggacg agcaggccac gctgggagcg
     4261 gtcggcgacc gacaggaagc gcccgcgggc gtcgaggtcg atcagcaacc gctgcgagtt
     4321 gcggatctgg tcgaaccggc cctgctcgat ctgccagtgg tcgatctctt gttgcggcgt
     4381 caggaccgga ctcgaccggc ggcgctcgcg gacgccgaaa cgcctgagct cgagccgcga
     4441 ccaggtgacg acgccggctg cggcgacgac cgcgaacagc gcgaaccagg ccagcagacg
     4501 aacgagccgc gacggtccgc cggcctgctc gacgagccgg tacgcgaagc tgaagccgag
     4561 gtaccaggcg aacaagctga ccaacggcag ccacaagtag gcgtagacca cccacagcac
     4621 gatcgtcaac acgccgtcgc gccggcggat cttctgtgtc agccggtcgc gggcgtcgat
     4681 cgtgcggtcg tcaaacccgg gtttgttcat agacgcgtct cgccagaccc cgatcggggc
     4741 tggtccaggt gccccggctc catttcggcc ggagcagcgc gcggggcagg gcatagaccg
     4801 aggtcacgac ctggatcgcc cagtagacca tcgggtacca gatcaaccag tagtacaccc
     4861 gcccgaggcg cgattcgtag cggctgtcga tcaccacgct gaccaggaac tgcagcagac
     4921 acatcgtgcc cagcagcagc ccgctccagc cggggaagac ggtcgcgccg gcgaacgggg
     4981 ccggtagatc gacgaagcgg ccggcgaccc acgtcgcgaa gaccgccgcc atcgcatagg
     5041 cccagaaggt gctggcgacg agctcggccg cgacgagcca catcctccgc gcgcgccagc
     5101 gaaacagcga cgggaagtgc ccgccgagca cctccatccc gccctgggcc cagcgcagcc
     5161 gctggcgcca caacccgcgc agggtctcgg gcatcaggat ccagcacagc gcgttcggct
     5221 cgtaacggat gtcccagtgc gcgagctgca ggcgccaact gacgtcgatg tcctcggtca
     5281 tcacctcgag gccccagtag cccacctggt gaacggccgc cttgcgaaac gccgccacca
     5341 cgcccgagac ggtgaaaaca cggccataga tccgctgcgc ccgcttgagc agtccgatga
     5401 tcgaggagaa ctcgccgacc tggatcttgc cgagcaacgt cgagcggttg cgcacgcgtg
     5461 ggttgcccgt caccgcgccg acccgcggac cgtcgacgaa gtggcgagcc agccacttcg
     5521 tcgcccgcgg cgtcagcagc gcgtcgccgt cgacggcaac caggatctcg tgcttggccg
     5581 ccagcgaagc gacacgcaga cccatcgcct tgccctggtt cgtgcggaag tgaatgacgc
     5641 gcacgcggta gtcctcgcgc gacagccgat cgagccgctc gccggtctcg tcggtgctcg
     5701 cgtcgttgac cgcgatgatc tcgaacaccg ggtagtcctg gtcgagcaac gactggatcg
     5761 tctcgaccac gtgcgtgccc tcgttgtgac acgggacgag gaagctgacc ggcggccact
     5821 gcgcgaggtc cggcggcgca tcggggtgcg caggcgaagc ccgttcgcga tagaagaagt
     5881 agtagaccga tcccgtgacc cacaggatcg acatgatcag cgggtagaag aaggtgaagt
     5941 cgaacagcgc cacgaccagc gcgtaccagc tcatcggtcg ccgctccgcg gcatgtcggc
     6001 cagcgacagg ccctgtctga gcagatcgac gcgcggatgc ccggcgacga gatcgtcggg
     6061 gtagtaggcc aggtgccgta cgccgtacga ctgcaggaag cggagcgtgt cgcgcagctc
     6121 gtccgacggg atcggccggc cggagcgcca gtcgacggtc tggagctgga acacggtctt
     6181 gtcgagaccc gcggcaaccc caccgaccgc ctgaatcagc cgacggtaga atcggcgctc
     6241 gcgctcggtg tcctccaacc gcggcatcgc catcaacgtc gtgtagtcgt agttcgccag
     6301 gtagtcggca tagtcctgcg cgagatagat ctccgcctcg ggatcgacca ccgccggggc
     6361 gaacaggttg cgcgaagtga gcgaccccgg gtgccacacc cgcacgatct cgaccaactc
     6421 gcggctgaac tcgatcaacg ccgcgctctt gatcgcggcc cagcgtcgtg ttcggtccgc
     6481 gtcgcggtcg acgccgtcga acgagaacgg ctcgcccgtc tcgcgctcgt aggccccgag
     6541 cgccgccggg ctgacgtcct cgaactcgtt cagccggccg tcgtcgtgaa agtggatgcc
     6601 gtcgagctgc gcgtggagcg cgaggtcgcg gtagatgtcg gcgatgaacc gccgggcgtc
     6661 ggggtggaac ggggagaggc gcgtctcgcc ggcggggtcc ggctgccggg tgtcgccggc
     6721 ctgctgcagc acgcgccagt ccgggtcgac cgcctcgccg cgataggcca acagcggtag
     6781 ccaggcgaag acccggacgt gcgcgcgcgt ccggagctgc cacgccgcac ggttgaacag
     6841 atcggcccgc acgggcagcc ggcgattggg gaaatagagc gcatccgccg cgccgtcgcc
     6901 gtccggatcg gcgaaggcct gcaggaagac gtggctgatc tcgagctgct cgatgcggtc
     6961 gagcagcaga ccgaggttga tctcctgccg ctcgggatcc gcgtcgtaga cgtaatccag
     7021 atcgacctgc gccgcccgca gcgggcgcgc ttggcgcggc gcgaacatcg cgctgaaccg
     7081 cgggatgccc gggttcgaca ccagcatctc gcgtccgaac acgccgtctc ggctggcgaa
     7141 ctcgacctcc tgcagcgtca gcgaaagctc catgcccagc ccggccgcga tctcgcggat
     7201 cggtcggttc cacgcaccga acggccaggc gatgacgcgc ggtcgccggc cggtgtggtc
     7261 ttcgatcgct cgcgcgctgg tcgacaggtc ggcggcgacg cgctcccggt agttcgcctc
     7321 ggactcgtag ccctcgtcgt cccaggccag cgcgacggcc gccggctcga ggttgccctg
     7381 cgggttggcg cggatcccgc ggtgtagatc gtgcgagtgc gacgcgatct cgaccagccc
     7441 cgaagcctgc atttcgcgaa tctgggccca gctcaggaag tcctccggcc cgagcgcgac
     7501 accgtcgtag tcgatcgcgc cctcggcctc gatccagctc gtcaccaagc tcaacaccgc
     7561 ggggtagcca aacgtctcga gtagcgggaa gacgtgggtg taggcgctgc ggaatccgtc
     7621 gtcgaaggtc aacagcacgg cccgtggcgg cagcggttca cccccggcca gcgccgccgc
     7681 gatccgctcg acgctgacca cgtggtagcc ctgcgaggcg agccagcgga agtgcgcagc
     7741 gagattggcg ctgctgaccg cgtagcggtc gggatcgtgg ttgcccacca ggtgcgaacg
     7801 cacgtcgtgg taggtgagcg tcacgagctc gacatcggcg gcgccggcgg ccgtgatcga
     7861 caacgcgagg aaggcgatgg cagcgcgcgt cacggcctgc cgtccacgcc gaacgtccag
     7921 aacgtcgcgt cttcgcggct gccgtcgtag acgcggcggt tccgctgcgc gccgaaccag
     7981 agctgccagc gggcgttgag gttccactgc agccggtaat cgagcgtcca aatcggatcg
     8041 gcgccgaatc cccgttgatc gtacaggccg gcgtcggcgc tcagccggtg cgtcaacgac
     8101 cgatcgtaga cgcgccactg ccgccagtcg ttggcgatgc cgatccgcgc aaccagcgcc
     8161 cgttccgggc tgaagtaggg gacgtcggcg tcgctgccgg tcgacgattc gacgagagcg
     8221 aagccgtcga ccttgtactt gtagccgttg tacaggcgca gacgcccgtc ggccgcgacg
     8281 gcggtcatcc ggttgccgtc gtcgtacggc tgatggccga gcgccagcga gacgtaccac
     8341 gattcactac cgcggtaggt gcccctggcg ctgaacaggt tgctgtcgat gccggcacga
     8401 tcggcgcgca gctgggtggc gtagctgtcg tattccgcga cgcagccgaa cagccagtga
     8461 tcggtgatgc tccaatcgag ccgcgcggcg gctcccggag agtcgaagtc gaagcggtca
     8521 ccgttgatct caccggcggc gcggagcccg ccgcggccat actcgacgcc ggcggccagg
     8581 cgtcggcgag cggtctcgcc gtcgtcgaac cgggcccacg agtcgaacgt gcgaacgtag
     8641 attcgatact cgtggccgag cggcgggctg aaccaccagc cgtcgatctg atactgctcg
     8701 ctgccgaaca cctcgcccga gctgttgccg taccgcgcgt cgatcgacag ccggctacgg
     8761 ttgtgggcgg cccagcgccg ctcgaggtcg cgcacggcga gcgtggccgg ctgctcgacc
     8821 ccgaggcgcg acagcgttcg gccggtgctg gccggccgtc ccaggtcgag gtcgttgtgc
     8881 gcggcggcga cgtcgcgcca cacgtcgccc accccaccgt ggtcgccgac ctgcacgtat
     8941 tgccgctcgg cgcggcggtg gaagccgcgc cagcgataga cgttgcccag ctcgtagcgc
     9001 gcctgagcgt tgttgggtgc tcgggccagc aggtgttcca accgccgctg ggcctccgcc
     9061 agttggtcgg cataggcccg tcccacggcc gcgatgatct cgacgtcgag ctggaccggg
     9121 ttcggttggc ccaccggcga tccgggggcc cgcggtgcgg gttcgagttc cgccgacagc
     9181 ccgtcgatca cgcggaacgc ctcgccgaac cgctcgagcg cgatcagcgc gtagaacagc
     9241 tcgatccgca gcgcgacgtt gccggggtcg atcgcctcgc ctcgtcgcag cgcccgctcg
     9301 gcctcccgcg gccgccgcaa cgcgaggtag gccgagccgg ccgcgatcag cgcgtaggtc
     9361 ggcggctccg cgccctcggc gatcaggctc tcgtaatcgg ccaccgccgg ctgaggccgg
     9421 ccggccgcca ccaggccgac gatccgatcg taacgcagcc gccgcgcgag ttcgccggtg
     9481 ctgggctcgg tggcgagccg tcggtcgatc gccgcgatcg cctcgtcgat gcgcgcgtac
     9541 ggagggccct ctccggcgga ctcgacggcg tcgcgcaggt cgagtgccag cgcgtcgccc
     9601 tcggtgcgag cgatctcccg ctcgtcgagg atgcccgggt gctggcgcgc cagctccagt
     9661 gcctgccgcg gcagcagcaa cgcgcgcagc gcctgcgcct tgccccgcag tgcctcgaag
     9721 ttgtccgggt cgacctcgag cacggcgtcg tactcgccga tcgcctcgag ctgggcgccg
     9781 tcgcgggtgt agagataggc cgacgacagg cgcacccgcg ccgtctgccg ggcggcgggt
     9841 tccaccgcct cgagcgtctg cctcgcgcgg cgcggctcgc cggcgtcagc cagcgacatc
     9901 gccagtccga cccgggcatc gacgctgtcg gggtcggcgg cgagggcgtg cccgtaccac
     9961 tcgaccgcca tcgcgaactg ttgcgtgttg cgcgcggaac cggcgaccgc ggagatcacg
    10021 taggccggtg ccaggcgcgg gtcgatccac ggcgcgttgc cgagcgcgtc ggtatggcgc
    10081 ccggcccaga acagcaccac ggtctggtcg tagagcacgg agggctcttc cgggtactcg
    10141 gcccgcagtc gttcgaacga ctcgagcgca cgttcgtact gccccgatcg tgccagctcc
    10201 acggcctctt cgcgtctctc gcgcaggctg ccgcgctcgg cttgcggatc gtcgcctcgg
    10261 gtggtcggcg ggtacgtgag caacaacccg atcgcgagcc cgagcccagt cccgatccgg
    10321 atgccgcggt tgtcgatgac cgtcgatccc cgtacggcgc tgccggccgc cccgaccctc
    10381 ctcgcgcgat ccgctcccgc ccaactatac cgcgacggga agtcgaacgg gcgccgcttc
    10441 gacgcgaagt gtggacggcg atgcgatctc ggtcgacaat gacggccgat gggtacggag
    10501 gtggcgaaaa tgctcgggcg tctcgtggtc ggtctgctgc tgctgtcggt cgcatcgcga
    10561 gcgccggcgg ccgaggccga tgcgcctcgg caagatccgt ggaaagggaa gacccgctcc
    10621 gaggtcgtcg gcctgttggg ggaacccgac aagacgaaaa ccgacaagca gggcgtgcta
    10681 tcgctgacct atcgattcaa ccggatcgac ccgatggcgc cgtcgcagcc ggatgcgatc
    10741 ttcctccagg tccccggggt cggcgtcgtc gcccgcatcg acaagtcgcg gcggccgccg
    10801 ccggacgcga tgacgatcga accgccgcag ttcgacgaac agggcaggca gaccgcgggc
    10861 ggcgtctcgg agacgcgctc ggcgacgacg tcctacgaca tcgactcgaa ggaggtatcg
    10921 cactcgacgt cggcatccgc cggcccgccc ggcgggagcc gggtcacgtt gcgtttcgag
    10981 atcggcagcg acgggcgcgt cgtcgactgg accaccaccg gcgagaaagg ccggtagtac
    11041 caccgcgggt gaggaaacac cgatgaatcg acgacccccc ggctgccgcc gagccacgcg
    11101 tgtcgcgctg ccgctgttgg tggtgctggc ctcggtcgcc gggctgccgg cgaagaaacg
    11161 cgagccgacg acgccgggcg agccgacgct cgccgcgctg acggaaggcg ccgagacgat
    11221 cgacgggctg ctgaccttct accggacgga ggacaagctg tacctgcggc tgccggccga
    11281 gctggtcggg gcgccgctgg ggttcgccgc ggtgcgggtc cacgccggcg gcgattacgc
    11341 gatgcgcggc ggagcgatcg acaaccaact cgtgcgctgg cgggtcgtcg gcgaccggct
    11401 ggtgctggtc aaggagaatc tggatttcag ggccgagcac gacccgacga ccgagcggct
    11461 gctcgagtcg tcgttcagcg actcgccggt cttctcggca ccgctcgtgc ggacgaacga
    11521 cgagcccgca ccgctcgtga tcgacgcgtc gaagctgttc gggcccgatc tggcgcaact
    11581 gctgccggac gacgccggtt acgacgtacg ggccgacgac gcgatcctcg cgtcgctcga
    11641 gtcgttcgac gacaacgtcg tggcccgcgt ggtctatcgc gcccaccggc aggccaagcc
    11701 cgcggagcag gcggccaacc cgttctcgcg cttcgtgcgg cccggacggc tgcccgacgc
    11761 gcgcgcggcc gagatcaccg tcgactacaa cttctaccgg ctggaggacg acggattccg
    11821 gccgcgagcc gccgacgagc ggatcggcgg gatgacgctg tcgtacaagg actactcgga
    11881 cgtcgacaac gccgacacgt tgttccgcca cgtgctccgc cgcttcgacc tacgcaaggt
    11941 cgatccgtcg gccgcggtgt cggacccgat cgagccgatc acgttctaca tcgatcgttc
    12001 ggtgccggag cgctggcgac cgctcgtgcg cgaggcgacc ctgtggtgga acccggcctt
    12061 cgaggcggtc gggctgcgca atgcgctgcg cgtgctcgac gccccggacg acccgcgctg
    12121 ggatccggcc gacatccgcc actcggtgat cttctggaac tactccgacg acctggtctt
    12181 ctccggcgtc gccgggccga tcttcgtcga tccgcgcaac ggcaagacga tcaaggcgac
    12241 ggtgcacctc aacggcgagt tcttctccta cgggctgcat cgttacctgg tctacgcctg
    12301 gtggcgagcg cccgcgccgg gcgcggacgc cacgacgtgg caggcccggc gggacgcggc
    12361 ccgcgcgttg cgccgcgcgc ccgccgcgtg tgaccgcgcg gcctcgttct cgagccagat
    12421 ggcgttcgcg cgtctggtgc tccgttcgcg cggggtgatc ggcgcggacc ccgccgagga
    12481 cgaccgtttc atccgcgagg cctttcgcga gctggtggcg cacgaggtcg gtcacgcgct
    12541 cggcttcccg cacaactgga aggcgagtct
//
LOCUS       DASPGN010000390         8334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1474521, whole genome shotgun sequence.
ACCESSION   DASPGN010000390 DASPGN010000000
VERSION     DASPGN010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8334
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474521"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VD788_11235"
     CDS             <1..627
                     /locus_tag="VD788_11235"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXV76877.1"
                     /translation="VGVRPGDPIVPHAEFRAMAIDGVVSGKAFDDRVGVALMCQAMSE
                     LAEREHPNTVIGVGAVQEELGARGAGTAAELARPDLALVLECTPADDLPPDKSPQAAL
                     GRGPQIRHYDPTAVSNRRLVRLVEQVAAEVGIEVQMAVRRSGGTDAGRIHTSGSGVPT
                     VVIGVPARYIHSHVAVMQLSDYRAALRLVVETVMTIDAEQARQFGSFD"
     gene            707..4060
                     /locus_tag="VD788_11240"
     CDS             707..4060
                     /locus_tag="VD788_11240"
                     /inference="COORDINATES: protein motif:HMM:NF028228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXV76878.1"
                     /translation="MMIASARSLPAVSCCLTLLWSGCGGGNGGRGEPAGVPEEVIRHN
                     IRGTAYLGQQKWAEAEAAFQQALDLRPHDALLANNQAVALIQQGRNDEAEQTLRRAIG
                     ESPDSPHARYNLGTILKNRGEFEAAAEQFEAVEAIDPDDLMTHYNLGVLYSRIGREAD
                     AEMQYRRALELDPTHVSTLYGLGRLLLQQGDQQRGVELISQSQAIRARSGLDEAVGTQ
                     YGEQGPYAMGADFPGDALAAPPPIEVEFDQSARAALASDRPPRAFVIGPAGAARDAVL
                     YLVDGDGSIRVLDGGGLGQAWPIGRPVWSIAVGDVDRDGTPEVIALAGDPNGLRPIVA
                     ARDGRVTEVAEPVPTPSGVAASALALVDQDHDGDLDLFFCWTAAAITDCAIATNAGSG
                     RFTVAPASDHGFSPRHLPDADRLSVDFSDVDNDRDVDLLLARRGRIDLFVNQREGRFE
                     ETSDAAGVGAVFPAADGALSVADVDKNGFMDLVAGVDRGTQVLANTGGRFDPGVVLAG
                     GPTEGSPNVVLDYDNDGFLDLASGGPDGVSLYRNLGAGEWETGGRRLTDRDGWPLAAL
                     DWDADGDLDLAILDRGESPAVVRLANRGGDRNRHIRIVSRGVGDNRDGVGAKVELLAG
                     ALRQKFEIKTAVPLHAGLGTRDRVESARYLWPSGVLQDEIDLPAGQPVEVTQLDRKGT
                     SCPLLYAWRDGAWRFVTDFLGGSAIGYLHAPGVYGRPDTDEYVKIEGGLSPAADGSLR
                     LRLNNQLQEVIWFDQTRLIVVDHPAGTEVFPDERLMPGPPFPAFRLWAADDLRPLARA
                     TSVGDGADLTAVLAERDRRYARNFELLPFKGYATEHTVELDLGRGLSRGERIVLLLDG
                     WIDYADSSANLAAHQAGRRLVPPELTVADGRGGWRSTDHRMGFPAGLPKTMAVELSGL
                     FPSDDLRVRIRTTMRIYWDRARVLVGGEDVELVVRVVEPSAARLEAGGYPAPTSPDGA
                     PPFGYDPAAVSFRSPWTAHVGRYTPFGDVTPLLRAIDDRFVTTRSGDQIELRFAAPDG
                     PAAGMERTFLLFADGFGKDMDLNSAASDTVGPVPFHGMSAYPYSAGITPPNAGPAEGA
                     AGRLVLDSDRGAPGAVPLALVTEDVAGPR"
     gene            4060..5871
                     /locus_tag="VD788_11245"
     CDS             4060..5871
                     /locus_tag="VD788_11245"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV76879.1"
                     /translation="MSGSGARRLALLLAAVAPLGCGDLGRGSNYDLVLTGGRVVDGVG
                     TRPYVADVAIRAGRIAAIGRLDPREAGRRIDVTGLVVAPGFIDMHSHADLILLAPIAV
                     QSELLEAKVRQGVTTVVVGNCGLGVAPAGVATAGILAAVNGWMTPRGVSAGPLGLADY
                     LDRIERGGTVVNAGTLVPHGPVRVEVLGVAAGEPSAAQLDRMRIEVDRGLAAGGFGLS
                     VGLIYPPGMYAATDELIELARVVARHDRLFTAHVRGASETLLDATSELVEIARRSGAR
                     VHHSHMEAVGEAFWAETKRMIAIEDRARADGWRISHDVFPYTRAATMMTAVFPPWALE
                     GGVDSLLERLREPRLRERMRAEIETLRPGWPPWTRDGWSHNLVGAVGWDGIFVASVGS
                     RDPHAPIGRTVAEIAGATGRRPFDVVVELLLDEHGAVGQQVAEISGRDGAIEALLAIL
                     EHPAAAIVSDAEDFGGGAPHPAHAGAFVRALRLNRELRLMPIEEIVRRMTGHPAGLLG
                     IGDRGRIEVGLAADLVAFDPLRVTDRASWDEPQRHAEGVELVLINGVPVVEAGAYSGA
                     AHGVVLRASDARLSSSSSPSTVSASTAAMRREAVPSP"
     gene            complement(5889..6935)
                     /gene="neuC"
                     /locus_tag="VD788_11250"
                     /pseudo
     CDS             complement(5889..6935)
                     /gene="neuC"
                     /locus_tag="VD788_11250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
     gene            7006..7539
                     /locus_tag="VD788_11255"
     CDS             7006..7539
                     /locus_tag="VD788_11255"
                     /inference="COORDINATES: protein motif:HMM:NF019032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HXV76880.1"
                     /translation="MRHARTGSRGLRWSIYGAAAVVALLLLNIDDWSRDFVSTHAEIS
                     AGAPESLRPFASTLPGDDLGLAVRWAAKRIGSWEFVGDAVEGRDRVLLFVRTSPLLRL
                     RDDILVRIEDRGHERVVTAVSEGRVAIGDLGRNPRNLRRLLVELRDVLDGASSRRAAP
                     VRSETGRRRSAPLARAA"
     gene            complement(7513..>8334)
                     /locus_tag="VD788_11260"
     CDS             complement(7513..>8334)
                     /locus_tag="VD788_11260"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV76881.1"
                     /translation="LLGLVLAWRRRGWPRLAIWFVAGYSASVVAFFVFSRFRMPMMPA
                     LYVLAAHAVVEIVRCLGRARVDRTRRARAALLVAGWLALVAFVHLPLRGRADSLAAAL
                     AGASGLPFRLETSATARFNLGVVYAARAADAADASAWLRRAEEQLRLAWAEEPRFASI
                     PIELGKVLARQRREAEAVEVYRAALALEPGNHRIHHALGLLAERMADEQAAETAFRRA
                     LELEPRHVASAVHLGESLLRQGRVEEARAAFAHAARLAPENERARSGLRRSREGR"
BASE COUNT         1153 a         2971 c         3040 g         1170 t
ORIGIN      
        1 gtcggcgtcc gcccgggcga cccgatcgtg ccccacgccg agtttcgtgc gatggcgatc
       61 gacggcgtcg tcagcgggaa ggcgttcgac gaccgggtcg gcgtcgcgct gatgtgccag
      121 gcgatgtcgg agctggccga gcgcgagcac cccaacaccg tgatcggtgt cggcgcggtg
      181 caggaagagc tgggcgcgcg cggcgcgggc acggcggccg agctggcacg accggacctg
      241 gcgctggtgc tcgagtgcac gccggcggac gatctccctc ccgacaagtc gccgcaggcc
      301 gcgctgggac gcgggccgca gatccggcac tacgacccga cggcggtgtc gaaccgccga
      361 ttggtccgcc tggtcgagca ggtcgccgcc gaggtcggca tcgaggtcca gatggcggtc
      421 cgccgcagcg gtggcaccga tgcgggacgg atccacacca gcggcagcgg cgtgccgacc
      481 gtggtgatcg gcgttccggc gaggtacatc cacagccacg tcgccgtgat gcagctgtcc
      541 gactaccgtg ccgcgttgcg actcgtcgtc gagacggtga tgacgatcga cgccgagcag
      601 gcccggcagt tcggcagttt cgactgagat tgcggcccgc gggctcgctt cggataatga
      661 cccgccaggc cgggcccgtg cgcgcggccg gacgcgaggc gaaccgatga tgatcgcgag
      721 cgctcgatcc ctcccggccg tgagctgctg cctgacgctg ctgtggagcg gctgcggcgg
      781 cgggaacggc ggccgcggcg agccggccgg cgttcccgag gaggtcatcc gccacaacat
      841 ccggggcacg gcctatctcg gccagcagaa atgggcggag gccgaagccg cgtttcagca
      901 ggcgctcgac ctgcgcccgc acgatgcact gctcgcgaac aaccaggcgg tggcgttgat
      961 ccagcagggt cggaacgacg aggccgagca aacgctgcgc cgcgcgatcg gcgagtcccc
     1021 ggacagcccc cacgcgcgct acaacctcgg cacgatcctc aagaaccgtg gcgagttcga
     1081 ggcggccgcc gaacagttcg aggcggtcga ggcgatcgac cccgacgacc tgatgacgca
     1141 ctacaacctc ggcgtcctct actcgcggat cggccgggag gccgacgccg agatgcagta
     1201 tcgacgggcg ctcgagctcg acccgaccca cgtctcgacg ctctacggat tggggcgact
     1261 gctgctgcag cagggcgacc agcagcgggg ggtcgagctg atctcgcagt cgcaggcgat
     1321 ccgggcacgg tcggggttgg acgaggccgt cggcacgcaa tacggcgagc agggacccta
     1381 cgcgatggga gccgacttcc cgggagacgc gctcgccgcc ccgccgccga tcgaggtcga
     1441 gttcgaccag agcgcgcgcg ccgcgctcgc gagcgaccgg ccaccgcgcg cctttgtcat
     1501 cgggcccgcc ggcgcggcgc gcgacgccgt gctgtacctc gtcgacggcg acggttcgat
     1561 ccgggtcctc gacgggggcg gtctgggcca ggcgtggccg atcggccggc cggtatggtc
     1621 gatcgcggtc ggcgacgtcg atcgagacgg caccccggag gtgatcgcgc tcgccggcga
     1681 cccgaacggg ctccggccga tcgtcgccgc tcgcgacggc cgcgtgacgg aggtggccga
     1741 acccgtgccg acgccatccg gcgtcgccgc ctccgcgctg gcactcgtcg atcaggacca
     1801 cgacggcgat ctcgatctgt tcttctgctg gaccgcagcc gcgatcaccg actgcgcgat
     1861 cgcgacgaac gccggctcgg gccgattcac ggtcgcgccc gcgtcggacc acgggttctc
     1921 gccgcggcac ctgccggacg ccgatcggct gagcgtcgat ttctcggacg tcgacaacga
     1981 ccgcgacgtc gacctgctgc tcgcgcgccg cggccggatc gacctgttcg tcaatcagcg
     2041 cgaagggaga ttcgaggaaa cgtcggatgc cgcgggcgtc ggcgccgttt tccccgcggc
     2101 cgacggcgcg ctgagcgtgg ccgacgtcga caagaacggg ttcatggacc tcgtcgccgg
     2161 ggtcgatcgc gggacgcagg tcctggcgaa cacggggggt cgtttcgacc ccggcgtcgt
     2221 gctcgccggc ggtccgacgg agggatcgcc gaacgtggtg ctcgactacg acaacgacgg
     2281 attcctcgac ctcgcctccg gcggcccgga cggtgtgtcg ctgtatcgca acctgggtgc
     2341 cggcgaatgg gagacgggcg ggcggcggct gaccgaccgg gacggctggc cgctcgccgc
     2401 gctcgactgg gacgcggacg gcgacctcga tctggcgatt ctcgaccgcg gtgagtcacc
     2461 cgcggtcgtc cggctcgcaa accgaggcgg cgaccgcaac cgccacatcc gcatcgtctc
     2521 gcgcggcgtc ggcgacaacc gcgacggggt gggagcgaaa gtcgagttgc tggcgggcgc
     2581 gctgcggcag aagttcgaga tcaagaccgc cgtcccgctg cacgccgggc tggggacgcg
     2641 cgatcgggtc gaatcggccc gctacctatg gcccagcggg gtgctgcagg acgagatcga
     2701 cctgcccgcc ggtcaaccgg tcgaggtcac tcagctcgat cgcaagggta cctcctgtcc
     2761 cctgttgtac gcgtggcgtg acggcgcctg gcgattcgtc accgacttcc tcggcggaag
     2821 cgcgatcggt tacctgcacg cgccgggcgt gtacgggcgt cccgacaccg acgagtacgt
     2881 caagatcgag gggggcctga gcccggcggc cgacggctcg ctccgcctgc ggctcaacaa
     2941 ccagctccag gaggtgatct ggttcgacca gacgcggttg atcgtcgtcg accacccggc
     3001 gggaaccgag gtcttccccg acgagcgcct gatgcccggt ccccccttcc ccgcgtttcg
     3061 gctgtgggcc gcggacgatc tccgtccgct cgcccgcgcc acctcggtcg gagacggagc
     3121 cgatctgacc gcggtgctcg cggagcgcga ccggcgctat gcgcgcaact tcgagctctt
     3181 gccgttcaag ggctacgcca ccgagcacac cgtcgagctc gacctcggcc gcgggttgtc
     3241 gcgcggcgag cgcatcgtgt tgctgctcga cggttggatc gactacgccg actcgtcggc
     3301 caacctggcg gcgcaccagg ccggccggcg gctggtgccg cccgagctga ccgtggccga
     3361 cggccgcggc ggctggcgct cgaccgacca ccggatgggc tttccggcgg gcctgcccaa
     3421 aacgatggcg gtcgagctga gcgggctgtt cccgtcggac gatctccgtg tccggatccg
     3481 caccaccatg cgcatctact gggaccgcgc gcgcgtgctg gtcggcggcg aggacgtcga
     3541 gctcgtggtt cgcgtggtcg aaccgtccgc ggcgcggctc gaagcgggcg gatacccggc
     3601 cccgaccagt cccgacgggg cgccgccgtt cggctacgac ccggcggcgg tttccttccg
     3661 ctcgccgtgg accgcccacg tcggtcgcta cacgccgttc ggcgacgtga cgccgttgct
     3721 gcgggcgatc gacgaccgtt tcgtcacgac ccgctccggc gatcagatcg agcttcgctt
     3781 cgcggcgccc gatgggccgg ccgccggcat ggagcgcacc ttcctgctgt tcgccgacgg
     3841 cttcggcaag gacatggacc tcaattccgc cgccagcgat acggtcggcc cggttccgtt
     3901 tcatggcatg tcggcctacc cgtacagcgc ggggatcacg ccgccgaacg cgggtcctgc
     3961 cgagggggcc gcggggcgtc tggtgctcga ctcggatcgc ggcgcgccgg gtgcggtccc
     4021 gctggcgctc gtcaccgagg acgtcgcggg cccgcgctag tgtcgggaag cggagctcga
     4081 cggctggcgc tgttgctggc ggcggtcgcg ccgctcggtt gcggtgacct cggccgcggg
     4141 tcgaactacg acctggtcct caccggcggc cgagtcgtcg acggcgtcgg cacccgaccc
     4201 tacgtcgccg acgtcgcgat tcgcgccgga cggatcgccg cgatcggacg gctcgaccca
     4261 cgggaagccg gcaggcggat cgacgtcacg gggctcgtcg tcgcgccggg cttcatcgac
     4321 atgcacagcc acgccgactt gatcctgctc gcgccgatcg cggtgcagtc cgagctgctc
     4381 gaggccaagg tgcgtcaggg ggtcacgacg gtcgtcgtcg gcaactgcgg cctcggcgtc
     4441 gcgcccgcgg gcgtcgcgac cgccgggatc ctcgccgccg tcaacggctg gatgaccccg
     4501 cgcggggtct cggcggggcc gctggggctg gcggactacc tcgaccgcat cgaacgcggc
     4561 gggacggtcg tcaatgccgg cacgctggtc ccgcacgggc cggtgcgggt cgaagtgctc
     4621 ggcgtggcgg ccggcgagcc gagcgcggcc cagctcgacc gcatgcggat cgaggtcgat
     4681 cgcggactgg ccgccggcgg gttcggtcta tcggtcgggc tgatctaccc gccggggatg
     4741 tacgccgcga ccgacgagct gatcgagctg gcccgcgtcg tcgcccgcca cgatcggctg
     4801 ttcaccgccc acgtccgtgg cgcgtccgag acgctgctcg atgcgacgag cgagctcgtc
     4861 gaaatcgcca ggcgcagcgg agcgcgggtc caccactccc acatggaggc ggtgggcgag
     4921 gcgttctggg ccgaaacgaa acggatgatc gcgatcgagg atcgggcccg cgccgacgga
     4981 tggcggatca gccacgacgt cttcccgtac acgcgggccg cgacgatgat gaccgccgtc
     5041 ttcccgccgt gggcgctcga gggcggcgtc gactcgctgc tcgagcgtct gcgcgagcct
     5101 cggctgcgcg agcgcatgcg tgccgagatc gagacgctgc gtcccggctg gcctccctgg
     5161 acgcgagacg gctggtcgca caacctggtc ggcgcggtcg gctgggacgg catcttcgtc
     5221 gcgagcgtcg gctcgcgcga tccgcacgca ccgatcgggc gcacggtggc cgagatcgcc
     5281 ggagccaccg gccgccggcc gttcgacgtc gtcgtcgagc tgctgctcga cgagcacggc
     5341 gccgtcggcc agcaagtggc cgagatctcg gggcgcgacg gtgcgatcga ggcattgctc
     5401 gcgatcctcg agcatccggc cgcggcaatc gtctcggacg cggaggactt cggcgggggt
     5461 gcgccgcacc cggcgcacgc cggtgcgttc gtccgagcgc tgcggctgaa ccgcgagctc
     5521 cggttgatgc cgatcgagga gatcgtccgg cgcatgaccg gccatccggc cggtctgctc
     5581 ggcatcggcg accgcgggcg catcgaggtc gggctcgcgg ccgatctcgt cgcgttcgac
     5641 cccttgcggg tcaccgaccg cgcgagctgg gacgagccac aacgtcacgc cgagggcgtc
     5701 gagctggtgt tgatcaacgg cgtgccggtc gtcgaggcgg gcgcttactc cggcgcggcc
     5761 cacggggtcg tgctgcgtgc gtcggatgcg cgcttgagca gcagctcctc gccgagcacg
     5821 gtttcggcca gcacggccgc gatgcggcgc gaggccgtcc cgtccccgta gggactggtc
     5881 atgccgagca gccgctcgcg gaagccggga tcgcgcgccc gacgcaccgc ctgcaggatc
     5941 cgctcggcgt cggcgggcac gtcgatcacg ttcggggcgc gctcccgtcc ctgctgccgg
     6001 atcccgacgt tgaccgtggg caccccgagc gacggagtct ccatgatgcc gctgctcgag
     6061 ttgccgagca tcaacgacac ctgccgcagc aggctccagt agactcgcgg gttgaggttg
     6121 acgaacaggc gggcatcgcc gtgctcggcg cagaaacgct cggctcgcgc gatcagctgc
     6181 cggctgcccg cgtcggcgtt cgggaaacag aacagtcgct gcccatcgat ccgttcgagc
     6241 gccccgaaca gcgcatccgc ctcgcgaacc gtgtcgcgcg cgatcgtcac cgggtgatac
     6301 gccacgagca tcgcgcccgg ccgcacgggc acgccgagcg cccgctcgag cgcctcgcga
     6361 cccagaggct ccgcccggtg caggtggtcg atcgacggtg cgccgacccg gtgcacgcgc
     6421 cagtgctcct cacccatcga cagcacgcgt cgctcggcac tcgccgtcgg gacgaaatgc
     6481 aggtggctca tcttggtcac ggcgttgcgg accgcgtcgt cgatcgcccc ctcactcacc
     6541 tccccgccct cgatgtgggc gatcgggatg cggagagcga gcgcgaccga ggccggcgcc
     6601 agcagctcgt agcgatcggc gatcaagagc aacagatccg gacgcaggcg ccccagcacg
     6661 tcggccagcc cgagcgtcgc cacgccgatc gtcttggcca tcgccgtgtc gctgtccgag
     6721 ctgatcaggc actcgacgcg ctcgtcgacg acgaacccgt cgcgctcgat ctcgtcggca
     6781 gcgcgaccga actcctcggc caactgggca ccgatggcga tcagctgcag atcgacccgt
     6841 gggtcgtcgc gcaggtcgcg cagcacccag ctcaggtggc tgtagtcggc gcgtgaggtc
     6901 gtgacgaccc cgatcctgcg ttgcggcgag cccatgctgc ctctccctcg ctcactatag
     6961 cccgcagtcg acgtcgcgtc gtccgtgacg ctagaatcga gcgagatgcg tcacgctcgg
     7021 acgggttcac gcggtctgcg ttggtcgatc tacggagcgg cggccgtcgt cgcgttgctg
     7081 ctgctcaaca tcgacgactg gagccgcgac ttcgtctcga cgcacgccga gatctcggcc
     7141 ggcgcgccgg agtcgctcag gcccttcgcg tcgaccctgc ccggcgacga tctcgggctc
     7201 gccgtgcgct gggcggcgaa gcggatcggc agctgggagt tcgtcggtga cgccgtcgag
     7261 ggtcgggatc gcgtgttgct gttcgtccgc accagcccgt tgcttcgtct gcgggacgac
     7321 atcctggtgc ggatcgagga tcgcggccac gagcgagtgg tgacggcggt ctccgagggc
     7381 cgcgtcgcga tcggcgacct cggtcgcaat ccgcgcaacc tgcggcgctt gctcgtcgag
     7441 ctgcgtgacg tgctggacgg cgccagctcg cggcgggcgg ctcccgtccg ctcggaaacc
     7501 ggccgacggc ggtcagcgcc cctcgcgcga gcggcgtaac ccgctgcgcg cgcgttcgtt
     7561 ctcgggcgcg agccgcgcgg cgtgggcgaa cgctgcgcgc gcctcctcca cgcgcccctg
     7621 tcgcagcagc gactcgccga gatggacggc actcgccacg tggcgcggct cgagctcgag
     7681 cgcccggcgg aacgccgtct cggccgcctg ctcgtccgcc atgcgctccg ccagcaaccc
     7741 gagcgcgtga tggatgcggt ggttgccggg ctcgagcgcg agagcggcgc ggtagacctc
     7801 gaccgcctcg gcctctcgtc gttgccgggc cagtaccttc cccagctcga tcgggatact
     7861 ggcgaagcgc ggctcctcgg cccaggcgag tcgcaactgc tcctcggccc gcctcagcca
     7921 ggccgaggcg tccgccgcgt cggccgcgcg cgcggcgtac accacgccga ggttgaaacg
     7981 ggcggtggcc gaggtctcga gacggaacgg caacccgctg gcgccggcca gcgccgccgc
     8041 caggctgtcg gcccgcccgc gcagcggaag gtggacgaac gcgacgaggg cgagccagcc
     8101 ggcgacgagg agcgcggcgc gggcgcgccg cgttcgatcg acccgcgcac gcccgaggca
     8161 ccggacgatc tcgacgaccg cgtgggcggc cagcacgtag agcgccggca tcatcggcat
     8221 gcggaaccgc gagaagacga agaacgcgac gaccgaggcg gagtagccgg cgacgaacca
     8281 gatcgcgagg cgtggccagc cacgccggcg ccaagcgagg acgaggccga gcag
//
LOCUS       DASPGN010000391         9762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_305824, whole genome shotgun sequence.
ACCESSION   DASPGN010000391 DASPGN010000000
VERSION     DASPGN010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9762
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305824"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VD788_11265"
     CDS             <1..693
                     /locus_tag="VD788_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76882.1"
                     /translation="LAGALDRSGVFVSDPAKKFQAGTVQAKVGALVGEARVRVIGDLP
                     WEFDFDGMQPGRAPQHWVRAGRPWEVREVDGGNVLVKLKRDSGLLRNELFMGPSTMTG
                     YTIQADVRGGKQGRRLTDIGLINNGYTLDLQGNLQKLEVRSWPAERRMAQAIDFAWEP
                     DTWYTMRMRVEITGDKGVIYGKVWPRGAEEPGEWTIRTEDPLPIPTGSAGLIGYSPAD
                     IYYDNIKVVVNE"
     gene            690..2204
                     /locus_tag="VD788_11270"
     CDS             690..2204
                     /locus_tag="VD788_11270"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV76883.1"
                     /translation="MKRQARTTATRQAEAMIAMVALLAPVAATADDAVMYGNTPARNM
                     ASSATGVPTKFDPDSGLNVLWSMPLGSQSYAGPLYYDGMVFVGTNNELELNSKFTGDR
                     GNLMAFDAATGKFLWQSAHAKLPAGRVNDWPLQGICSTPAVENGRVYYVSNRAEVICA
                     DAEGFRDGENDGPVTDEPSKTEIDEDVIWKYDMIGQLDVFPHNLAAGNPLLVGDLLFT
                     VTGQGVDEGHVNIPSPAAPSFVALDKNTGELVWESALPGTKVLHGSWSNPGWIEFDGK
                     GQVVFPGGDGVLYGFEPASGELIWQFDMNPADSVWELGGRGTRNNVISTPVVDDGLIY
                     IGVGQDPEHGEGWGNFWVLDVAGKTGKLDEKAVVWHVGGEKFGRTLSTAAIHDGLTYI
                     ADLSGFVYCFDAKTGKLHWKYDTFAAIWGSPFYVDGKVFIGDEDGDLAVLKAGRGKDG
                     EPELLHEVNMGASVYTTPVVHDGVMYVVSRNRLFALKEGIAPKKKDAPPTEKKG"
     gene            2213..3301
                     /locus_tag="VD788_11275"
     CDS             2213..3301
                     /locus_tag="VD788_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76884.1"
                     /translation="MAVTDRTLEGRTAQARERLDAHVREMVRWHFDPATGCPFWLDKA
                     VEWGFDPRSEIGGYDDLKLLGHFKDEWLRGGPIRRWVPKGLAGRPLYVFETGGSTGVP
                     KSRVNVEDFQRDYEIFGDNLDDATFPPGSDWLMLGPTGPRRLRLAIEHLAHHRGGACF
                     HVDLDPRWVSKLVREKRWKEVDEYKNHCIDQALTVLRAHDDIHCLFTTPKLLEALAEK
                     VSLRKAGITGIFCGGTEMTAQFNRFAREELVPGIDFVPTYGNTLMGLASPKPFDPADG
                     YSITYYPPLPRAVFEIVDPKNLDRTVDYDERGRVLLTTLTHEFFMPRFPERDEGYRAR
                     PSDRFPWDGVRDLGLLTELQASVAVGVY"
     gene            3301..4740
                     /locus_tag="VD788_11280"
     CDS             3301..4740
                     /locus_tag="VD788_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV76885.1"
                     /translation="MLHVPVVRAGRSYRSLDRVTLTHVRTSEPVVEVSQANAGLIARD
                     LLRVGEHRRRLQAIGVEPLLDICRRAAESFIDGELPVDVDSGSTLGPEAFVELQSATT
                     GMPQALCRANMEKVRFVLAEMPRVLGGLSRGLDLRALDDGWVEQNGRPVSYLCQTDAL
                     GAVLPSNSPGVHALWIPAIPLKVPLVLKPGREEPWTPLRIMHSLIAAGCPAEALSFYP
                     TDHAGTSQILLRCQRSMFFGDATTVAAWQGEDRIQLHGPGWSKVVLGPDKVDSWPQYI
                     ELMTTSIAANGGRSCVNASGVWVPAHGRAIADALAARLASIEPRPLDDPQAELAAFTR
                     PEMARAVSDYIDDLLAQGGAEDVTAAHRRGGRVIEVDGCTFLLPTLIHCSDPDHPLAR
                     TELLFPFAAVVEVPPAELLDRIGSTLVVSAVTDDPRFRRELLTSRNVERLNLGPLPTG
                     VVSWDQPHEGNLFEHLYRQRAFQAADPAA"
     gene            4754..5920
                     /locus_tag="VD788_11285"
     CDS             4754..5920
                     /locus_tag="VD788_11285"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXV76886.1"
                     /translation="MAGIEINPRDLSETGSWSVSAESGRLVYGEGSIDSIGEQVRALD
                     RFRALVVTDRGIRAAGHVERAERSLAAAGVAYSIFDGVVENPTTVNVELGREFASGWA
                     PDCIVALGGGSSMDCAKGINFLYTNGGRMQDYWGTGRATRAMLPSVGVPTTAGTGSEA
                     QSYALISDATTRVKMACGDAKARFLTVILDPSLTPSAPFEVRAVSAIDAASHATESYV
                     SSRRNPVSQLFAREAWRLIDTSLERSLAESGDVGARGRMLLGAHFAGRSIEHSMLGAA
                     HALANPLTARFGLTHGIAVGLMLPHVIRFNRDEVDGLYGELEAVSADAGEPIDRRLER
                     FRRAGRLPATLGEVGVPLSALDQLAADAASQWTANFNPRRVGERELRVLYEAAY"
     gene            5904..7061
                     /locus_tag="VD788_11290"
     CDS             5904..7061
                     /locus_tag="VD788_11290"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV76887.1"
                     /translation="MKPLTDRRAGPLAIAWVAALGSVVPASASALPAGDDWPMFRGDA
                     GLAGVSRTTLAERLEPLWVFEVEEGIESAVAIVDGTVYLGALDGNLYAIDLDDGEVKW
                     KYEAGAEIRSSPTHAAGSIYFGDGDGIFHAVDATRGTARWKFETGGEIVSSANVSADR
                     VLFGSHDQFLYCLDADDGSLLWKIETEGYVYGTPALVDGKAISVGCDGFLRAVRLEDG
                     KEVQRFEIGSYVGASPATVAGKAYFGTFDNQVMAVDLGDGRVEWVYQNPERQFPYLSS
                     AAVHGGIVIVGGRDKLVHAIDAETGKSLWTHATRGKVDASPVVAGDRVYVASAGGEIL
                     GLDLASGKVDWSFDSGFGIVASPALSGGRLVIGTTEGRLFCFGASGSKDTR"
     gene            7058..8416
                     /locus_tag="VD788_11295"
     CDS             7058..8416
                     /locus_tag="VD788_11295"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HXV76888.1"
                     /translation="MRRDEVKTAPERSEPNTAVGADPKETGLGSNFVSNYPPYSFWKE
                     EDVRLADTAFDREPRDRTPLGLYLHIPFCRKRCKFCYFKVFTDKRGDEVQRYLDAVVR
                     EVASYAGRAAVAGRPLEFVYFGGGTPSFISARHLRVLVAGLKHELDWSAVREVTFECE
                     PGTLTEAKLHAIREVGVTRLSLGIENFDDRILAENGRAHTTREIYRVEPWIRQIGFEQ
                     LNIDLIAGMVGESWDSWRETVRRTLEMAPDSVTVYQMELPYNTVYSQRLLDGREVGIA
                     DWATKRAWHAYAFEQLSRLGYRGSSAYTMVRADRDVRFVYRDSVWHGCDLLGIGVSSF
                     GHISGVHYQNESRWDPYLDAVESGRSAIARAFVTDASERLVRETILLLKRGYLDARAL
                     GQKHGADVVERFRPVVAELSDRGLMTLSGDRIELTREGLLRVDQLLPHFYHPRYQNAR
                     YT"
     gene            8509..>9762
                     /locus_tag="VD788_11300"
     CDS             8509..>9762
                     /locus_tag="VD788_11300"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXV76889.1"
                     /translation="MMGSRVVLILLLLAVGGLCVPGYAEDEPADEREPDEGAAASGTP
                     TAEDEYIEVNVSYVPKSSAIVSKLPLSQQMTPANVGTVSEALIFEQDGYVLSDALRNV
                     SSLNVQAQAGVHDYFLIRGYDSISGSMVMTDGAIEPEATWYPTYNIVGVEVLKGPSGF
                     LYGVDPLAGVVNIVRKQPLPTNFATFGLSMGSFATGEGVVDWNHSSADGKKNFRLNAL
                     GYGTDNWRDVGRNRQYAVNPSFGWQVGQDSQLNFNLEAVRAEFSPDSGIPIVDGGLPS
                     VPRDRSYQTPFDFSDQDIVRFQLDYQTRVNDAVTLRNKFYVRDLDWTTKGTQFLGVGL
                     NPFEPTGITRTLTSLDDDQQYVGNQIEGVFTVTTGPVTHNLLTGLEIRYFTDTFDIGV
                     LPPEDPQNPLNPGVPSIDLFDPVETA"
BASE COUNT         1636 a         3154 c         3384 g         1588 t
ORIGIN      
        1 ctggccggcg cgctcgatcg gtcgggggtc ttcgtgtcgg acccggccaa gaagttccag
       61 gccgggacgg tgcaggcgaa ggtcggcgcc ctcgtcggcg aggcgcgggt ccgggtgatc
      121 ggcgacctgc cgtgggaatt cgacttcgac gggatgcaac cgggccgcgc gccccagcac
      181 tgggtccgtg ccggccgccc gtgggaggtt cgcgaggtcg acggcggcaa cgtgctggtc
      241 aagctcaagc gagactcggg tctgttgcgc aacgagttgt tcatgggacc gagcacgatg
      301 acgggctaca cgatccaggc cgacgtccgt ggcggcaagc agggccgccg gctgacggac
      361 atcgggttga tcaacaacgg ctacacgctc gatctgcagg gcaacctgca aaagctcgaa
      421 gtgcggtcgt ggcccgcgga gaggcgcatg gcccaggcga tcgacttcgc gtgggagccg
      481 gacacctggt acacgatgag aatgagggtc gagatcacgg gggacaaggg agtgatctac
      541 ggcaaggtgt ggccgcgcgg cgcggaagag ccgggtgaat ggacgatccg caccgaggac
      601 ccgctgccga tcccgaccgg gagcgccgga ttgatcgggt actccccggc ggacatctac
      661 tacgacaaca tcaaggtggt ggtgaacgaa tgaagcggca agcacgaacg acagcaacgc
      721 ggcaggcgga ggcgatgatc gcgatggtgg cgctcctggc gccggtcgcc gcgacggccg
      781 acgatgcggt gatgtacggc aacactccgg cccggaacat ggcctcgtcc gcgaccggag
      841 tgccgaccaa gttcgacccc gactcggggc tgaacgtctt gtggtcgatg ccgctcggct
      901 cgcagagcta cgccgggccg ctgtactacg acggcatggt cttcgtcggc accaacaacg
      961 agctcgagtt gaactcgaag ttcaccggcg accgcggcaa cctgatggcg ttcgacgcgg
     1021 cgaccggcaa gttcctctgg caatccgctc acgccaagct ccccgcggga cgcgtcaacg
     1081 attggccgtt gcagggcatc tgctcgacac cggccgtcga gaacggacgc gtttactacg
     1141 tctcgaatcg tgcggaggtg atctgcgccg atgcggaggg cttccgcgac ggcgagaacg
     1201 acgggccggt cacggacgag ccgagcaaga ccgaaatcga cgaggacgtg atctggaagt
     1261 acgacatgat cggccaactc gacgttttcc cgcacaatct cgccgcgggt aatccgctgc
     1321 tcgtcggcga cctgctgttc accgtcaccg gacaaggcgt cgacgaggga cacgtcaaca
     1381 tcccgtcgcc ggcggcgccg agcttcgtcg cgctcgacaa gaacaccggc gagctggtgt
     1441 gggaaagcgc gctgcccggc accaaggtcc tccacggcag ctggtcgaat cccggctgga
     1501 tcgagttcga cggcaaaggc caggtggtct tccccggcgg ggacggcgtg ctctacgggt
     1561 ttgaaccggc atcgggcgag ctgatctggc agttcgacat gaacccggcg gactcggtgt
     1621 gggagctcgg cggacgaggc acccgcaaca acgtgatctc gacgccggtc gtcgacgacg
     1681 ggctgatcta catcggcgtc gggcaggacc cggagcacgg cgagggctgg ggcaacttct
     1741 gggtgctcga cgtcgccggc aagaccggca agctcgacga gaaggcggtc gtctggcacg
     1801 tcggcggcga aaagttcggc cgcacgctgt cgactgccgc gatccatgac ggcttgacct
     1861 acatcgccga cctgagcggc ttcgtctact gcttcgacgc caagaccggc aagctgcact
     1921 ggaagtacga cacgttcgcc gcgatttggg gctcgccgtt ctacgtcgac ggcaaggtgt
     1981 tcatcggcga cgaggacggt gatctcgcgg tgctcaaggc cggccggggc aaggacggcg
     2041 aaccggagct gctgcacgag gtcaacatgg gagcgtccgt ctacaccacg ccggtggtcc
     2101 acgacggcgt gatgtacgtc gtcagccgca accgcctgtt cgcgttgaag gagggtatcg
     2161 ccccgaagaa gaaggacgcc ccgccgaccg agaagaaagg gtagggcggc cgatggcggt
     2221 gacggaccgg acactcgagg gccgaacggc gcaagcgcgc gagcgactcg acgcccacgt
     2281 gcgcgagatg gtgcggtggc atttcgatcc ggcgaccggc tgtccgttct ggttggacaa
     2341 ggccgtcgag tgggggttcg accctcgcag cgagatcggt ggctacgacg acctcaagct
     2401 gctcgggcat ttcaaggacg agtggctgcg cggcgggccg atccggcgct gggttccgaa
     2461 ggggctcgcc gggcgaccgc tctacgtctt cgagaccggt gggtcgaccg gggtgcccaa
     2521 gagtcgcgtg aacgtcgagg acttccagcg cgactacgag atcttcggcg acaacctcga
     2581 cgacgcgacc tttccccccg gctccgactg gttgatgctc gggccgaccg gaccgcgccg
     2641 gctgcggttg gcgatcgagc acctggccca ccatcgcggg ggggcgtgct tccacgtcga
     2701 tctcgatccg cgctgggtgt cgaagctcgt tcgcgagaag cgctggaagg aagtcgacga
     2761 gtacaagaac cactgcatcg accaggcgtt gaccgtgctg cgcgcccacg acgacatcca
     2821 ctgcctgttc accacgccga agctgctcga agcgctggcc gagaaagtgt cgctgcgcaa
     2881 ggcgggcatc accggcatct tctgcggcgg aacggagatg accgcccaat tcaaccgctt
     2941 cgcgcgtgag gaactggtcc cggggatcga cttcgttccg acctacggca acacgctgat
     3001 gggtctggcg tcacccaagc cgttcgaccc ggccgacggg tactcgatca cctactatcc
     3061 gccccttccg cgcgcggtgt tcgagatcgt cgacccgaag aacctcgacc ggaccgtcga
     3121 ctacgacgaa cgcggtcgtg tcttgttgac cacgctgacg cacgagttct tcatgccgcg
     3181 cttccccgag cgggacgagg gctatcgtgc gcggccgtct gatcgattcc cgtgggacgg
     3241 cgtgcgcgat ctcggcctgc tcaccgagct gcaggcctcg gtcgccgtcg gagtctactg
     3301 atgctgcacg tgcccgtggt ccgcgccggc cgttcctacc gcagtctcga ccgcgtcacg
     3361 ctgactcacg tccgaacgtc ggagcccgtc gtcgaggtca gccaggcgaa cgccggactg
     3421 atcgcacgcg acctgctgcg ggtcggggag caccgccgcc gactccaagc gatcggcgtc
     3481 gaaccgctgc tcgacatctg ccggcgtgcc gccgaatcgt tcatcgacgg cgagcttccg
     3541 gtcgacgtcg acagcggcag cacgctgggg cccgaggcct tcgtcgagct ccagtcggcg
     3601 acgaccggca tgccgcaggc gctgtgtcgc gcgaacatgg agaaagtgcg cttcgtgctg
     3661 gccgagatgc cgcgagtgct cggtggattg agccgcggac tcgatctgcg cgcgctcgac
     3721 gacggctggg tcgagcagaa cgggcggccg gtcagctact tgtgccagac cgatgcgctc
     3781 ggcgcggtgc tgccgagcaa ctcgcccggc gtgcacgcgc tgtggatccc cgcgattccg
     3841 ctcaaggtgc cgctcgtact caaacccggt cgggaagaac cctggacacc gctgcgcatc
     3901 atgcactcgt tgatcgccgc cggctgcccg gccgaagcgt tgagcttcta cccgaccgac
     3961 cacgcgggga cgagccagat cctgctgcgc tgccagcgct cgatgttctt cggtgacgcc
     4021 acgaccgtcg cggcctggca gggcgaggat cggatccagc tgcacgggcc gggatggagc
     4081 aaggtcgtcc tgggcccgga caaggtcgat tcctggccgc agtacatcga gctgatgacg
     4141 acgtcgatcg cggccaacgg cggtcgctcg tgcgtgaacg cctcgggtgt gtgggtcccc
     4201 gcgcacgggc gcgcgatcgc cgacgcgttg gccgcccggc tggcctcgat cgaaccacgc
     4261 ccgctcgacg acccgcaagc cgagctggcc gccttcaccc gccccgagat ggcgcgcgcg
     4321 gtctcggact acatcgacga cctgctcgcg cagggtggcg cggaggacgt gaccgccgca
     4381 caccgccgcg gggggcgcgt catcgaagtc gacggttgca ccttcctgct gccgacgttg
     4441 atccactgca gtgacccgga ccacccgctg gcgaggacgg agctgctctt tccctttgcc
     4501 gcggtggtcg aggtcccgcc ggcggagctg ctcgaccgca tcggctcgac gctggtcgtc
     4561 agcgcggtga cggacgaccc gcggttccgg cgcgagctgc tgacgtcgcg caacgtcgag
     4621 cggctcaatc tcggtccgct gccgaccggt gtcgtctcgt gggatcagcc gcacgagggg
     4681 aacctgttcg agcacctgta tcgccagcgg gccttccagg ccgccgatcc cgcagcctga
     4741 gacgggatcg gacgtggccg gcatcgagat caacccgcgg gacctgtcgg aaaccggctc
     4801 gtggtcggtc tcggccgaat ccggccggct ggtctacggc gaaggttcga tcgactcgat
     4861 cggcgagcag gtgcgtgcgc tcgatcgctt ccgcgcgttg gtcgtgaccg atcgcgggat
     4921 tcgcgccgcg ggccacgtcg agcgcgcgga gcggtcgctc gccgccgccg gtgtcgccta
     4981 ctcgatcttc gacggggtgg tcgagaaccc gacgaccgtc aacgtcgagc tgggtcgcga
     5041 gttcgccagt ggctgggcgc ccgactgcat cgtcgcgctc ggcggtggca gctcgatgga
     5101 ctgcgccaag ggcatcaact tcttgtacac caacggcggt cgcatgcagg actattgggg
     5161 caccggtcgg gcgacccgcg cgatgctacc ctcggtcggc gttccgacga ccgccggcac
     5221 cggctccgaa gcccagtcgt acgcgctgat atcggacgcg acgactcggg tcaaaatggc
     5281 gtgcggcgac gccaaagccc gattcctcac ggtcattctc gatccgtcgc tcacgccgag
     5341 cgcgccgttc gaggtgcgcg ccgtgtcggc gatcgacgcc gcctcccatg ccacggagag
     5401 ctacgtttcg tcgcgacgca acccggtatc gcagctgttc gcgcgcgagg cgtggcgtct
     5461 gatcgacacg agcctagagc ggtcgctggc ggagagcggg gacgtcggcg cacgcgggcg
     5521 catgctgctc ggggcccatt tcgccggtcg ctcgatcgag cactcgatgt tgggtgcggc
     5581 ccacgcgctg gccaatccgc tgaccgcgcg gttcgggctg actcacggca tcgcggtcgg
     5641 actgatgctg ccgcacgtca tccgcttcaa tcgcgacgag gtcgacgggc tgtacggcga
     5701 gctggaggcg gtttcggccg atgcgggaga gccgatcgat cgccggctcg agcggttccg
     5761 ccgggccggc cgtctgccgg cgaccctcgg cgaggtcggc gtcccgctga gcgcgctcga
     5821 ccagctcgcc gccgacgccg cgagtcaatg gaccgccaac ttcaacccgc gccgcgtcgg
     5881 agaacgggag ttgagggtgc tctatgaagc cgcttactga tcgacgggcc ggcccgctgg
     5941 cgatcgcgtg ggtcgcggcc ctcgggtccg tcgtgccggc atcggcttcg gcgctgccgg
     6001 cgggcgacga ttggccgatg ttccgtggtg acgccgggct cgccggcgtc agccgtacca
     6061 cactcgccga gcggctcgag ccgctgtggg tcttcgaggt cgaggagggg atcgagtccg
     6121 ccgtcgcgat cgtcgacggc acggtctatc tcggggcgtt ggatggcaat ctctacgcga
     6181 tcgatctcga cgacggcgag gtcaagtgga agtacgaggc cggtgccgag atccgatcat
     6241 ccccgaccca cgccgcgggc tcgatctact tcggagacgg cgacggcatc ttccacgccg
     6301 tcgatgccac ccgcggcacg gctcgctgga aattcgagac cggcggcgag atcgtctcgt
     6361 cggccaacgt ctccgcggac cgcgtgctgt tcggctcgca cgaccagttt ctctactgtc
     6421 tcgatgccga cgacggttcg ctgctgtgga agatcgaaac cgaaggttat gtctacggca
     6481 cgccggcact ggtcgacggc aaggcgatct cggtcggttg cgacggcttc ctgcgtgccg
     6541 tacgactcga ggacggcaag gaggtccagc gcttcgagat cggctcctac gtcggcgcca
     6601 gtccggcgac ggtcgcgggc aaggcctact tcggcacctt cgacaaccag gtgatggcgg
     6661 tcgatctggg cgacggtcgg gtcgagtggg tttaccagaa cccggaacgg cagtttccgt
     6721 acctctcgtc ggccgcggtg cacggcggga tcgtgatcgt cggtggccgg gacaaactgg
     6781 tgcacgcgat cgatgccgaa acgggcaagt cgctgtggac gcatgcgacg cggggaaagg
     6841 tcgatgcttc acccgtcgtc gccggtgacc gggtctacgt cgcgtcggcc ggtggcgaga
     6901 tcctcggtct ggacttggcg agtggcaagg tcgactggag cttcgacagc ggattcggca
     6961 tcgtcgcctc ccccgcgctg tccggcggac ggttggtgat cggcaccacc gagggtcgcc
     7021 tgttctgctt cggcgcgagc ggttcgaagg acacgagatg aggcgggacg aggtcaagac
     7081 ggctccggag cggagcgaac cgaacaccgc ggtcggcgcc gatccgaagg aaaccgggtt
     7141 gggcagcaac ttcgtctcga actacccgcc ctattcattc tggaaggaag aggacgtccg
     7201 gctggccgac acggcgttcg accgcgaacc gcgcgaccgc acgccgctcg gtctgtacct
     7261 gcacatcccg ttctgccgca agcgttgcaa gttctgttac ttcaaggtct tcaccgacaa
     7321 gcgtggcgac gaggtccagc gctatctgga cgccgtcgtt cgcgaagtgg cgtcgtacgc
     7381 cggccgtgcc gcggtcgccg gtaggccgct cgagttcgtt tacttcggtg gcgggacgcc
     7441 gtcgttcatc agcgccagac atctccgcgt gctggtcgcg ggtctgaagc acgagttgga
     7501 ctggagcgcc gttcgcgaag tgaccttcga gtgcgagccc ggcacgttga ccgaggcgaa
     7561 gctgcacgcg atccgcgagg tcggcgtgac gcggctcagt ctgggcatcg agaacttcga
     7621 cgaccgaatc ctcgccgaga acggacgggc acacacgaca cgcgagatct accgcgtcga
     7681 accctggatc cgccagatcg gcttcgagca gctgaacatc gacctcatcg cggggatggt
     7741 gggggagagt tgggatagct ggcgcgagac ggtgcggcgc acgctcgaga tggcgccgga
     7801 cagcgtcacg gtctaccaga tggagttacc ctacaacacg gtctactcgc aacgcctgct
     7861 cgacggtcgc gaggtcggta tcgccgattg ggcgaccaag cgggcgtggc acgcctatgc
     7921 cttcgagcag ttgtcccgtc tcggctaccg cgggtcgagc gcctacacga tggtccgcgc
     7981 cgaccgcgac gtacgcttcg tctatcgcga ctcggtctgg cacgggtgcg atctgttggg
     8041 gatcggtgtg tcatcgttcg gtcatatctc gggtgtgcac taccagaacg agtcgcgttg
     8101 ggatccctac ctcgatgcgg tcgagtccgg ccgctcggcg atcgcgcggg cgttcgtcac
     8161 ggacgcgtcg gagcgtctgg tgcgagagac gatcctgctg ctcaaacgcg gctacctcga
     8221 cgcgcgggcg ctcggccaaa agcacggggc cgacgtcgtc gagcgcttcc ggccggtcgt
     8281 cgcggaactg agcgatcgcg gcttgatgac gctgagcggg gatcggatcg agctgacccg
     8341 cgaagggctg ttgcgcgtcg accagctgct gccgcacttc taccatcccc ggtaccagaa
     8401 tgcacgctac acctaggctc cccgacgcga gtgcatcgta gacgagagac gagaggcggt
     8461 tgacggtgtc ggaaggggcc gagcacaaac ggcggtcgag agggagccat gatgggtagt
     8521 cgagtcgtgc tgattctgct gttgttggcg gtgggcgggc tgtgcgtgcc ggggtacgcc
     8581 gaggacgaac cggccgacga gcgcgagccg gatgagggtg ccgcggccag cgggacaccg
     8641 accgccgagg acgagtacat cgaggtcaac gtatcctacg tccccaagtc gagcgcgatc
     8701 gtctccaagc tcccgttgtc gcaacagatg acaccggcca atgtcggcac ggtcagcgag
     8761 gcgctgatct tcgagcaaga cgggtacgtg ctgagcgacg cgctgcgcaa cgtcagcagc
     8821 ttgaacgtcc aggcgcaggc gggcgtgcac gattacttct tgatccgcgg ctacgattcg
     8881 atcagcggga gcatggtgat gaccgacggc gcgatcgagc cggaggcaac ctggtacccg
     8941 acctacaaca tcgtcggcgt cgaggtgctg aagggtccct cgggctttct ctacggcgtc
     9001 gatccgctgg cgggagtcgt caacatcgtg cgcaagcagc cgcttccgac caacttcgcg
     9061 accttcggtc tgtcgatggg gagtttcgcc accggcgagg gagtggtcga ctggaaccat
     9121 tcgagcgccg acgggaagaa gaacttccga ttgaacgcgt tgggctacgg aacggacaac
     9181 tggcgcgacg tgggtcggaa ccggcagtac gccgtcaatc cgagcttcgg ctggcaggtc
     9241 ggccaggaca gccagctcaa cttcaacctc gaggcagtgc gagcggagtt cagcccggac
     9301 agcgggatcc cgatcgtcga tggcgggctg ccgtcggttc cacgcgatcg ctcgtatcag
     9361 acacccttcg atttcagcga ccaggacatc gtccggtttc agctcgacta ccagacgcgc
     9421 gtcaacgatg cggtgacgct gcgcaataag ttctacgtgc gcgatctgga ctggaccacc
     9481 aagggaacgc agttccttgg cgtcggcctc aacccgttcg agccgacggg gatcacgcga
     9541 actctgacct cgctcgacga cgaccagcag tacgtcggca accagatcga gggagtgttc
     9601 acggtcacaa ccggcccggt cacgcacaat ctgctcaccg gtctcgagat tcgttacttc
     9661 accgacacct tcgacatcgg cgtcctacca cccgaggatc cgcagaaccc actgaatccc
     9721 ggcgtgccga gcatcgacct gttcgacccg gtcgagaccg cc
//
LOCUS       DASPGN010000392         6066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_890347, whole genome shotgun sequence.
ACCESSION   DASPGN010000392 DASPGN010000000
VERSION     DASPGN010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890347"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1610)
                     /locus_tag="VD788_11305"
     CDS             complement(<1..1610)
                     /locus_tag="VD788_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV76890.1"
                     /translation="MSETRVNPLVRFAVDRRVTMGMIVLGVGVLGWLSLTRLPLEFLP
                     AFSSSNITVIAPYSSSSPDEIERRIVRPLEDSLGTINGVDTLSSSASSDSARVSVGFI
                     DGTDMDLAAVEVRDRIDRVRHLLPDDLDRVFVRRFQSSDIPVLRLDLSATWPDARLYE
                     FAETVVQRRLERLEGVAQVEVDGLRVPELQVNLDQARMLAHGVDVRSLVTLLRESNVN
                     LSGGDIREGSRRLLVRAEGEFRTLEQVRDQPIRANGLRLRDVADVAYTFPEKEEYNYL
                     NGVEALTVSINKTSGANLLEVVDRAKRELADIERLSEFAGASIRVYFDASQDVREGLG
                     QLRDAGTIGGLLAILAVYLFLRRVRTTLLVGLSIPVSVVATFVLIYLMRQAGLLDITL
                     NVISLAGLMLALGMLVDNSVVVIESVFRHRNDLDEDSRTAALRGASEVALPIIASTAT
                     TMCVFLPLIFLGSGGRFKLYFENIGVTICIVIVASLLVALTIVPMAAAVMLRGQSARP
                     APFIDRLTRWYGSTLRFTLNHRLGFVIS"
     gene            1962..2975
                     /locus_tag="VD788_11310"
     CDS             1962..2975
                     /locus_tag="VD788_11310"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXV76891.1"
                     /translation="MNLQTAFTRQVGIEVPLICGAMYPCSNAALIAAVSEAGGIGIVQ
                     PISLTYVHGHELRAGLREIRARTARPIGFNAIVERSSRIYQDRMRRWVDVAIEEGVRF
                     FVTALGNPRWVVDRVRAVNGVVYHDVTDRRWALKALEQRVDGLICVNRRAGGHTGTRD
                     GRELFEELHDLGVPLICAGGIGDETTFAEAIRLGYAGAQLGTRFIASHECTAHDDYKR
                     AIVEARAEQIVLTDKLSGVPCSVIRTPYVDRVGTRSGRLARWLLRGRRTKHLMRTIYA
                     LHSLRRLKHASLRGLSYRDIWQAGKSVETIDRIEPAGEIVRRFRDAIGRADRQPAAAI
                     EPR"
     gene            3116..3550
                     /locus_tag="VD788_11315"
     CDS             3116..3550
                     /locus_tag="VD788_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76892.1"
                     /translation="MSEGINTEVRHGSRVFHVQTQTADRREPVIETLIYEGGAVAVRM
                     TASLAALSEKWRVPAGDVSRLLALQHWEMVHKIKHGMLDGAAAERPSDAPAPARSDPA
                     AVERLDASIDRDAPGVRELLAELEAKLDRLVTGGDPSEPDED"
     gene            3645..3977
                     /locus_tag="VD788_11320"
     CDS             3645..3977
                     /locus_tag="VD788_11320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HXV76893.1"
                     /translation="MDTYYCPHDLTRFAEMGENRPELWAKYIDYYNAVFEDGALTERE
                     KSLIALAVAHSVQCPYCIDAYTRSCLEKGADLDQMTEAVHVANAIRGGSSMVHGVQMR
                     SVAKKLGM"
     gene            3979..5013
                     /gene="arsS"
                     /locus_tag="VD788_11325"
     CDS             3979..5013
                     /gene="arsS"
                     /locus_tag="VD788_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274671341.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HXV76894.1"
                     /translation="MTSLLQIGHPLASPAAQLDMLERTGAPAFDDVLSRHSLAALESG
                     PRIEVLQVNVGKLCNQTCAHCHVDAGPDRKEIMTREVAQQVIELLRRHPIPTLDITGG
                     APELNPNFRYLVERARELGRQVIDRCNLTVLAIRGQHDLPRFLAAHAVEVIASLPSFR
                     RAGTDAQRGDGVFAKSIDGLRKLNAVGYGRGGGLRLTLVHNPVGAFLPGGQSSLEREY
                     KRALERDHGVVFDTLYTITNMPISRFLEFLDRSGNLDRYMKLLVDSFNPRAVGAVMCR
                     SYISIGWDGRLYDCDFNQMLALPVDHGAPATLGALLASGDLSRRVRTERHCFGCTAGH
                     GSSCAGAVSG"
     gene            complement(5017..>6066)
                     /locus_tag="VD788_11330"
     CDS             complement(5017..>6066)
                     /locus_tag="VD788_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76895.1"
                     /translation="AFGGEPCTVVQWSSWSDASAIAPFAMQALLYHDSLLIVFQIRPG
                     DQTLAGGTTGIQQRDARSASRYPATDAGVGDDTAVCWFEPRYPAGPPAADLELINADK
                     VDSVVAGHYVHYAVAVRNHGPAPAGAVDLHDVVPPTLENCRWFCSASAGSSCAAEGTG
                     PIDESVAVAAGGWVGCDLHCRVATGSAASEIAHHAAAEISGPVADPLPANNSATDVNQ
                     LLAEPPECGSAAAAAVVDALGDPSDLVPRALHAGPGPLTVTVQRVFALDAEPAPASER
                     LAASCAAAADGRPPPLGSGDRPAGQTAFQIEFTIEDGRGGVCSAIASVRGTSARGRFG
                     LTPAAVSAGDACALP"
BASE COUNT          963 a         2098 c         2121 g          884 t
ORIGIN      
        1 atcgagatga cgaaccctag tcggtgattc agcgtgaagc gcaacgtcga gccgtaccag
       61 cgggtcaggc gatcgatgaa cggggcgggc cgcgcgctct gtccgcgcag catcaccgcc
      121 gccgccatcg gaacgatcgt cagcgcgacc agcaacgagg ccacgatcac gatgcagatg
      181 gtgactccga tgttctcgaa gtacagcttg aacctcccgc ccgagccgag gaagatcagc
      241 ggcaggaaga cgcacatcgt cgtcgcggtc gaggcgatga tcggcagcgc gacctcactg
      301 gctccgcgca gcgcggcggt ccgcgagtct tcgtcgaggt cgttgcggtg acgaaagacc
      361 gactcgatga cgaccaccga gttgtcgacc aacatgccga gcgccagcat cagcccggcc
      421 agactgatca cgttcagcgt gatgtcgagc agacccgcct gccgcatcaa gtagatcagc
      481 acgaacgtcg cgaccacgct gaccgggatc gacagcccga cgagcagcgt cgtgcgcacg
      541 cgccgcagga acaggtagac cgcgaggatc gccagcagcc cgccgatcgt gccggcgtcc
      601 cgcagctggc ccagaccctc gcgcacgtcc tgcgaggcat cgaagtagac gcggatcgac
      661 gcgccggcga actcactcag ccgctcgatg tcggccagct cgcgcttcgc ccgatcgacg
      721 acctccagca agttggcgcc cgacgtcttg ttgatgctga cggtcagcgc ctcgacgccg
      781 ttcagatagt tgtactcctc cttctcgggg aaggtgtagg ccacgtcggc cacgtcgcgc
      841 agccgcaacc cgttcgcgcg gatcggttgg tcgcggactt gctcgagcgt gcggaactcg
      901 ccctcggcgc ggacgagcaa ccggcggctg ccttcgcgga tgtcgcctcc cgacaggttc
      961 acgttgcttt cgcgcaacag ggtgaccagc gatctgacgt cgacgccatg ggccagcatc
     1021 cgtgcctggt cgagattgac ctgcagctcg ggcacgcgca ggccgtcgac ctcgacctga
     1081 gccaccccct ccagccgctc gagcctgcgc tggacgacgg tctcggcgaa ctcgtacaac
     1141 cgcgcgtcgg gccaggtcgc gctgaggtcc aatcgcagca ccgggatgtc cgagctttga
     1201 aaccgtctga cgaatacgcg gtcgaggtcg tcgggcagca ggtgtcgcac gcggtcgatc
     1261 cgatcgcgca cctcgaccgc cgccaggtcc atgtcggtcc cgtcgatgaa gccgacggag
     1321 acgcgcgccg aatcggacga cgcgctggac gacagcgtat cgacgccgtt gatcgtgccc
     1381 agcgagtcct cgagcgggcg gacgatccga cgctcgatct cgtcgggcga ggacgaggag
     1441 tacggtgcga tcacggtgat gttcgaggac gagaacgcgg ggaggaactc gagcgggagc
     1501 cgggtgagcg acaaccagcc cagcacgccg acaccgagca cgatcatgcc catcgtcacc
     1561 cggcggtcga ctgcgaagcg gacgagcgga ttgacgcgcg tttccgacac agtttcgccg
     1621 tgacccctga cggggcctga agcccctcgc tcgggtttct ctcgactatc ggacgaaccc
     1681 ggaacgccgc tgggatcata tccgaccgcg gcccgcaccg gcgacccggc gccggccgct
     1741 cgtgtaactg tttgtggaca cgggtcttgc gctcgagaac ctggcggcgc ggacgacggg
     1801 ttcccacggc cgttgcgatc gacgggcgac cgtgtgaata atcgccgcac cggcacggga
     1861 tccgggcgat ggtcgaggcg ttcccgctcg acgacgcacg gcgggtctct cggcggctgt
     1921 ccgtcggctg acgtgtgttt cggggcggcg ctcgtggtgg agtgaacctg caaaccgcgt
     1981 tcacgcgaca ggtcgggatc gaggtgccgc tgatctgcgg cgcgatgtac ccctgctcca
     2041 acgccgcgct gatcgccgcg gtctccgagg ccggcggcat cggcatcgtc cagccgattt
     2101 ccctcaccta cgtgcatggt catgagctgc gggcgggctt gcgcgagatc cgcgcgcgga
     2161 cggcgagacc gatcggcttc aacgcgatcg tcgagcggtc gtcgcggatc taccaggatc
     2221 ggatgcgacg ctgggtcgac gtcgcgatcg aggaaggcgt gcgcttcttc gtcaccgcgt
     2281 tgggcaaccc gcgatgggtc gtcgaccgcg tccgggcggt caacggcgtg gtctatcacg
     2341 acgtgaccga tcgtcgctgg gcgctcaagg cgctcgagca gcgggtcgac gggttgatct
     2401 gcgtcaatcg gcgtgcgggg gggcacaccg gcacgcggga cgggcgcgag ctgttcgagg
     2461 agctgcacga cctcggcgtc ccgctgatct gcgccggcgg catcggcgac gagaccacgt
     2521 tcgccgaggc gatccgtctg gggtacgccg gagcgcagct cggcacgcgg ttcatcgcca
     2581 gccacgagtg taccgcccac gacgactaca agcgcgcgat cgtcgaggcg cgagccgaac
     2641 agatcgtgtt gaccgacaag ctgagcgggg tgccgtgctc ggtgatccgc acgccctacg
     2701 tcgaccgcgt cggcacgcgc tccggccggc tggcccgctg gctgctgcgt ggccggcgga
     2761 cgaagcacct gatgcgcacg atctacgcgc tgcactcgtt gcgccggttg aagcacgcgt
     2821 cgttgcgcgg cctgtcctac cgtgacatct ggcaggccgg caagagcgtc gagacgatcg
     2881 atcggatcga gcccgccggc gagatcgttc gacggttccg cgacgccatc ggcagggcgg
     2941 atcggcagcc ggcggcggcg atcgagccgc gctagtcgtc gacgaacggc cgctgcccga
     3001 gccacgcggc cgaacgggga gcgccggctg cgctcgccgt cgtcgaccgg cccgtcggca
     3061 gcccgccgct tgaccgccga gacgactacg ccgattgttg ccgggcgagg tccgcatgag
     3121 cgaagggatc aacaccgagg tccggcacgg ctcccgcgtg ttccacgtgc agacccagac
     3181 cgccgaccgt cgcgagccgg tcatcgagac gttgatctac gaaggcgggg cggtcgccgt
     3241 gcggatgacc gcctcgctgg cggcgttgtc ggagaaatgg cgcgtgccgg cgggcgacgt
     3301 ctcgcggctg ctcgcactcc agcactggga gatggtgcac aagatcaagc acgggatgct
     3361 cgacggagcc gccgcggaac ggccgagcga cgcgccggcg cccgcgcggt ccgacccggc
     3421 cgcggtggag cgcctcgacg cgtcgatcga ccgggacgcc cccggcgtgc gcgagctgct
     3481 ggcggagctc gaggccaagc tcgatcgcct ggtcaccggc ggcgacccgt ccgagccgga
     3541 cgaggactga gcgggaacaa ccggccgcgg gccgctgtta gcgaggaagc cggccgatgg
     3601 tacagtgtcc gaccgccccg acgtccgccg gcaggagacc gcgagtggat acctactact
     3661 gtccgcacga cctgacccgc ttcgccgaga tgggtgagaa ccggcccgag ctgtgggcca
     3721 agtacatcga ctactacaac gcggtgttcg aggacggggc gttgaccgag cgcgagaaga
     3781 gtctgatcgc gctggccgtc gcacactcgg tgcagtgccc gtactgcatc gacgcctaca
     3841 cccggtcctg cctcgagaaa ggcgccgatc tggaccagat gaccgaggcg gtccacgtcg
     3901 ccaacgcgat ccgcgggggc tcgtcgatgg tccacggggt gcagatgcgt tccgtcgcca
     3961 agaagctggg catgtgagtt gaccagtctg ctgcagatcg gccacccgtt ggcctcgccc
     4021 gcggcgcagc tcgacatgct cgagcgcacc ggcgcgccgg cgttcgacga cgtcctgtcg
     4081 cgtcattcgc tcgccgcgct cgaaagcggg ccgcgcatcg aggtgctgca ggtcaacgtc
     4141 ggcaagctgt gcaaccagac ctgcgcgcat tgtcacgtcg atgccggccc cgaccgcaag
     4201 gagatcatga cgcgcgaggt cgcccaacag gtgatcgagc tgctgcggcg ccacccgatc
     4261 ccgacgctcg acatcaccgg gggggccccc gagctgaacc ccaactttcg ctacctggtc
     4321 gaacgggcgc gcgagctcgg acggcaggtg atcgaccgtt gcaacttgac cgtgctggcg
     4381 atccgcgggc agcacgatct gccccggttc ctcgccgcgc acgcggtcga ggtgatcgcg
     4441 tcgctcccgt cctttcgtcg ggcaggcacc gacgcacagc gcggcgacgg cgtgttcgcc
     4501 aagagcatcg acggattgcg caagctcaac gccgtcggtt acgggcgagg cggcgggctg
     4561 cggttgaccc tggtgcacaa cccggtcggt gcgttcctgc ccggcggcca gagctcgctc
     4621 gaacgcgagt acaagcgcgc gctcgagcgc gatcacggcg tcgtgttcga cacgctgtac
     4681 acgatcacca acatgccgat cagccgcttc ctcgagtttc tcgaccgcag cggcaacctc
     4741 gaccgctaca tgaagctgct cgtcgactcg ttcaacccgc gggccgtcgg cgcggtgatg
     4801 tgccggagct acatcagcat cggctgggac ggacggctgt acgactgcga tttcaaccag
     4861 atgttggcgc taccggtcga tcacggggca cccgccacgc tcggcgcgtt gctggcgtcc
     4921 ggcgatctat cgcgccgcgt gcgcaccgag cgtcactgtt tcggttgcac cgccggccac
     4981 ggctcgtcgt gcgccggcgc ggtcagcggc tgacgatcac ggcagcgcgc aggcgtcgcc
     5041 ggccgacacc gccgccggcg tgagaccaaa ccggccgcgc gccgacgtgc cgcgcaccga
     5101 agcgatcgcg ctgcagaccc ctccgcgccc gtcctcgatc gtgaactcga tctggaacgc
     5161 ggtctggccg gcggggcgat caccgctgcc gagcggcggc ggtcggccgt cggccgcggc
     5221 ggcgcacgat gcggccagcc gctcgctcgc cggcgcgggc tcggcgtcga gcgcgaagac
     5281 gcgctggacc gtcaccgtca gcggacccgg gccggcgtgg agcgcgcgtg ggaccaggtc
     5341 cgacggatcg cccagcgcgt cgaccacggc ggcggccgcc gcgctgccgc actcgggcgg
     5401 ttcggcgagc agttggttga cgtcggtcgc cgagttgttg gcgggcagcg ggtcggcgac
     5461 gggccccgag atctcggcgg ccgcgtggtg ggcgatctcg ctcgccgccg agccggtcgc
     5521 cacgcggcag tgcagatcgc agccgaccca gccgcccgcc gccaccgcga cgctttcgtc
     5581 gatcgggccc gtgccctcgg ccgcgcacga cgaccccgct gacgcactgc aaaaccaccg
     5641 gcaattctcc agcgtcggcg gaacgacgtc gtggaggtct accgcgccgg cgggcgccgg
     5701 tccgtggttc cgtaccgcga ccgcgtagtg cacgtagtga ccggcgacca cgctgtcgac
     5761 cttgtcggcg ttgatcagct cgaggtccgc ggccggtggt cccgcggggt agcgcggctc
     5821 gaaccagcag acggcggtgt cgtccccgac gcccgcatcg gtcgccggat agcggctggc
     5881 ggagcgcgcg tcgcgctgtt ggatgcccgt cgttccaccg gcgagcgtct ggtcaccggg
     5941 gcggatctgg aaaacgatca gcaggctgtc gtgatacaac agcgcctgca tcgcgaacgg
     6001 agcgatcgcc gacgcgtcgc tccaactcga ccattgcacg acggtgcaag gctcgccgcc
     6061 gaacgc
//
LOCUS       DASPGN010000393        12466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1006718, whole genome shotgun sequence.
ACCESSION   DASPGN010000393 DASPGN010000000
VERSION     DASPGN010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12466
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006718"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VD788_11335"
     CDS             <1..677
                     /locus_tag="VD788_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXV76896.1"
                     /translation="MTEALVALFARAADTPRLKAVLLEGEGANFSFGASVEQHLPGSF
                     ERMLPGFHAIFDRMLACAVVTLAAVRGQCLGGGLELAAFCHRVFAAPDARLGQPEIAL
                     GVVAPVASVILAERIGRGRAEELCLTGRSYTAREALAAGLVDQVDDDPTAAALEWACR
                     YLLPRSSSSLRFAVRAVRTGFAGRFRQDLAEVERLYAEGLMETTDAAEGLRAFLEKRP
                     PQWSDR"
     gene            674..1852
                     /gene="bcrC"
                     /locus_tag="VD788_11340"
     CDS             674..1852
                     /gene="bcrC"
                     /locus_tag="VD788_11340"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit C"
                     /protein_id="HXV76897.1"
                     /translation="MSDNDRRLLVERAEGLARDMTFAEVRAWKRRTGGLAIGFMPVYV
                     PRELLHAQGVLPVGVVGGGDEVEIIRGDAYYQSYICHIPRSTIELGLNGGLDCLDGMI
                     FPAICDVIRNLSGVWKLEFPHKLAYYLDLPQNFDRSIGGRFYRDELCRLSAELTERGA
                     RQYDGELLRASIELYNEQRRLVQRLYDLRREQPWRVPTAEAYVLLRAGLVVAPEEFSR
                     MLADYLRAASADDRCKPMDQAKVVLVGSFCEQPPLGLIKTLERSGCYIVDDDLVQVHR
                     WLRREVATAGDPLDALTAALLDHAIDSPTRYSPNGANGQDLVERVHRSGAEGVLFCAP
                     SFCDPALLDQPMAVGAVERAGIPWTAFKYSENGGQFQVIREQAGTFADSIKLWSEVHR
                     "
     gene            1849..3141
                     /gene="bcrB"
                     /locus_tag="VD788_11345"
     CDS             1849..3141
                     /gene="bcrB"
                     /locus_tag="VD788_11345"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HXV76898.1"
                     /translation="MSEPTVTGTPAALKDDSQLRQKRMIAEHYTRLARSPQTGEKSVY
                     TFVPGNLTELLRSFDLLPLLPEINALQSAMRGKSREYIAAAERLGHSEDVCTYVKCDV
                     GMLRSGNVGPTGEVLPDPAMLLLSYTGCFTFMKWFEILRREYRCPTVMLHVPYQADGA
                     VTDSMRRYVVDQLTDVVIPALERLSGRRYDQERLSEMLARSALAEDDLVWVLRSAARR
                     PSPIDAYFGGVYYIGPIFSAFRGTEEAVDYYRALREEVSRRAARGEGPVTPDGVIDRE
                     RYRIVVEGPPNWTHFREFWKMFADEGAVVVASTYTKVGGVYDFGFRHDPSRPLETLAD
                     YCMGCYTNLNLPGRVDMLCRYVEEYSADGLLVNSIKSCNSFSAGQLRILREVERRTGK
                     PAGFIESDLVDPRYFSAANIKNRLESYFQMIEQKRSAA"
     gene            3138..4460
                     /locus_tag="VD788_11350"
     CDS             3138..4460
                     /locus_tag="VD788_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HXV76899.1"
                     /translation="MRYSVGIDLGSTTTKAVIVGESGAILGRGITNSRSNYRVACDVA
                     LGEALISARFTLVADEWRRAGGAGADDGALLGRLELGFREQQYRAQLATLAEVIDRLS
                     RSVADADAVRPAVAWILEQMERETPGLYATDGQRRSDFFRDLAGARYMQLGEQVARGR
                     EVGFDRLAGLFDKAILEVENSPVAAGDFAGHAGRALERMAPVPEKLVGAVARAARARL
                     EQAASVGTGYGRQTLPFPKDKIRSEILCHGLGAHAMFPATRTVLDIGGQDTKAIQVDE
                     NGVVTSFQMNDRCAAGCGRYLGYIADEMNLGVHQLGPLACSARRAVRINSTCTVFAGA
                     ELRERLSLGEPREDILAGLHRAIILRAMSLLARSGGVYDQFTFTGGVARNPAAVGALE
                     RLVDENYGRLTTNISPDSIYTGALGAALFARRGLARDGRDAAGGGARA"
     gene            4457..5326
                     /gene="bcrD"
                     /locus_tag="VD788_11355"
     CDS             4457..5326
                     /gene="bcrD"
                     /locus_tag="VD788_11355"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit D"
                     /protein_id="HXV76900.1"
                     /translation="MTRETVGPVAGVDVGSSAVKVVVLDQPAGAEPCTLFADSSRIRR
                     RDPQRVANELFRTALTTIGLEPEQLTYVASTGDGELLDFRTGHFYGMTTHARGGLFLD
                     PEARAVIDIGALHARAVLMDERAKVLGYRMTSQCASGSGQFLENICRYLGITIEEIGE
                     LSTRADDPEPCSSICAVLAETDVINMVSRGISTANIIKGIHVSMAGRYLRLLASVGAR
                     GVVLVTGGLAGDAGLLQALREGVEAQKSKLPIEIRAHVHSALAGAYGAALWGAFRARK
                     LGGAGARFAAGAQ"
     gene            5323..6066
                     /locus_tag="VD788_11360"
     CDS             5323..6066
                     /locus_tag="VD788_11360"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXV76901.1"
                     /translation="MNATARPPAPLSLEGRTGIVSGGAGGIGSAVVRTLVGAGARVAS
                     VDLPGREAPAGATAIPCDLSDAAAIDRMFADFDGRFDRLDLLVHCAGVTRDAVLWKMS
                     ADDWSRVMRVNLDAAFHLLRGAAPRLRAAGRGAVVLISSINGERGKFGQANYAASKAG
                     LIGLGRTAARELGRFGVRVNVVAPGWVETAMTAALPAEVKRQAIGESPLGRTATPEDV
                     AAAVWFLCAEMSRHVTGQVLRVDGGQLMA"
     gene            6089..6580
                     /locus_tag="VD788_11365"
     CDS             6089..6580
                     /locus_tag="VD788_11365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV76902.1"
                     /translation="MMEATDEREDRVVVRGLRPADLDAVIALDAKNVGRRREQFFRLK
                     LEQNLSETGIKVSLAAELDACFVGFLLARVYYGEFGSLEPVAVLDTIDVHPDFRGHGV
                     GSALMRQLRVNLVALGVSQLRTEVGWDSQQLMRFLHREGFRPAARVCLDLPLEWERVG
                     RRC"
     gene            6574..7002
                     /locus_tag="VD788_11370"
     CDS             6574..7002
                     /locus_tag="VD788_11370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXV76903.1"
                     /translation="MLTLSKSTRYALYATMEMARAAGDGHVTAAEVAGRYKIPPSVLA
                     KVFQQLVRSGLARGSRGSGGGYRLARAAGEITVLEVMRAFQPPNGAVPSAAGEPAAAA
                     PLTRLLDEVAELARCTFASVTLETLTRTARPVHRVPSAGR"
     gene            complement(6988..8262)
                     /locus_tag="VD788_11375"
     CDS             complement(6988..8262)
                     /locus_tag="VD788_11375"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HXV76904.1"
                     /translation="MSDRPARSRQLDAATLESILEVTRRLAAPFELDEILARIIDAGR
                     AVLAADRGTVFLLDETTDELVVRVGTGVRELRIPAGKGIAGECAQSRRLINVPDCYAD
                     ARFNRDVDRESGYRTRCLLAVPLIAEQDRLVGVLQLLNKKRGTFSQRDERIASALAAQ
                     CAVALERVRMTQELLEKERMQRELAVAREIQTGFLPRKLPRVDGYDLAGVTEPAEETG
                     GDTFDWIGSGPRGAVMLVGDATGHGVGPALSATQVRAMVRIAGRLGASLEQTLAHVND
                     QLTEDLAANRMVTAFLGRLDADAGKLFYHAAGQGPLLHYHAATDQCEWRNSTAPPLGC
                     IASLPVPKPAEFVLAPGDVVGVMTDGIFEYADARERMFGETGVAEVVRRHHDRPMSEL
                     VRLLFDAARRHADGRPPADDITIVLVRRLPSG"
     gene            complement(8294..10297)
                     /locus_tag="VD788_11380"
     CDS             complement(8294..10297)
                     /locus_tag="VD788_11380"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXV76905.1"
                     /translation="MNRALVSRGRAVILLALVPGLLGWLCLRWPVTGALETRYGFDLL
                     MQLRGVRQPPPAVCVVAIDDASAIELGHDPLDPWPRAVHARLIGVLAREGARAIAVDV
                     LFDEPRDPAQDVELELALFDAGNVVLAATVERVEDPRFRSTRRIDPHPPFAESAAAVA
                     SVELPTDADGVVRRAWLVLDERPSLALAAYRVATGGDAPRADGAARLIDYYGPPRTIP
                     TVSLYQALDPDQYLPAGYFRDRIVFVGASQPAALTPSAAKDSFPTPFSGGDVGLTYGV
                     EIHATVAANLVDGSEIRLPLRAVETLLVLALALLASLLFIVLRPLAGAAMLCGLELLA
                     WSGAYFAFVAGGLWLPVVIPSVVQLPAAYVVSLVWYYVTTARDREKIRRAFAFYLSPE
                     MIRRIAENPEALSLGGEEVVATAVFTDIRGFTRIAERMGARETAAMLNRYFSEVTGRI
                     FDTGGTLIKYIGDAVFAIWGAPVPFDDHATRACRAAIEIARAEQAPGDGAGSELITRI
                     GIHTGSMLVGNLGSAQRFDYTAIGDTVNLAARLESLNKQFGTRALVSAETLGATDGTF
                     VTRALGRVRVAGREEPVAIHELLGQNGAVTRPDAETIGRFERAVGDFVAGGFERAAEG
                     FREVRERCGGRDGPSELYLDAIARRRAEPTAGEWDGVIRFTEK"
     gene            complement(10347..>12466)
                     /locus_tag="VD788_11385"
     CDS             complement(10347..>12466)
                     /locus_tag="VD788_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76906.1"
                     /translation="QTAVILDPENSLYRSYLGKAYFEERRKDAAEKELEIAKQLDPSD
                     PTPYLYGALVKRTYNRPVEALKDLQKSIELNDKRAVYRSRLLLDEDLAVRSADLAGTY
                     NDLGFDQLGMVTARRSADIDQSNHSAHLFLAGTYRRLPGFAPAFLSETLQARIYQPVN
                     VNAVRPDVVNESVSFNEYTSLINRNRVRGFATIGYGEVDTDLENSALAPLEIGDSNPS
                     GGEATVSFNRDRLAGALSYESFEDDGFRINNDVDNKALRGFLAFAPSYRDQFQLSFID
                     GTRETGDLPLRAVPFIPGIERIETELTNVGLGYHRIISPAADLAVSLIYNDTRQTAVN
                     RFDGQPTGSAQLEGPQIEAQYVRRQTWLTWTAGVGHFDGEQTADTVVLGQPTSASADD
                     EFSNGYLYAKLRKLGPVELTAGASYEEVTAPVGFLPPRDSGVLPGDVPYEDNQLSPKL
                     GLSIQGTPTTVIRATAYSRLNPAIGRLQTLEPTQVAGFNQFFDDPAGTSSDNYGVGLD
                     QQFAKGFFGGLGVLRRDLEIPEPTCENPDPTIGCGFAQPSSQIVERTSDDWLGNVYLN
                     ATAGRRVALSLEYAYEQREFDFTQVDNLSRFEDSMRTHRVRPQVRYFLPMGFFAGVQA
                     TYYDQTVDQFTDLSSDVRTANENDFWIGDVELGYLFPQRWGSVSLRVFNVTDQDFDYY
                     RSSLEESVIPARTVLLSLSFTSP"
BASE COUNT         1889 a         4224 c         4416 g         1937 t
ORIGIN      
        1 aaatgaccga agcgctcgtc gcgctgttcg cccgcgccgc ggacacacca cggttgaagg
       61 ccgtgctgct cgagggtgag ggagcgaact tctcgttcgg ggcgagcgtc gagcagcatc
      121 tgccgggcag cttcgagcgg atgctccccg gcttccacgc gatcttcgat cggatgctgg
      181 catgcgcggt cgtgacgttg gccgcggttc gcggacagtg tctcggcggc ggtctcgagc
      241 tggccgcgtt ctgccaccgt gtcttcgccg cgcccgacgc gcggctgggt cagccggaga
      301 tcgcgctggg cgtcgtcgca ccggtggcct cggtgatcct cgccgagcgc atcggtcgcg
      361 gccgcgccga ggagctctgc ctgaccggtc gcagctacac cgcgcgcgag gcgctggccg
      421 ccggcctggt cgatcaggtc gacgacgacc cgaccgccgc ggcgctcgag tgggcctgcc
      481 gctacctgct gcctcgctcg agcagcagcc tgcgattcgc cgtgcgcgcc gttcgaaccg
      541 gcttcgccgg acgattccgc caggacctcg ccgaggtcga gcgcctgtac gccgagggct
      601 tgatggagac gacggacgcc gccgagggtc tgcgcgcttt cctcgagaag cgaccgccgc
      661 agtggagcga ccgatgagcg acaacgaccg acgcctcctc gtcgagcgcg ccgaagggct
      721 ggcgcgggac atgaccttcg ccgaggtgcg ggcgtggaag cgacgcacgg gcggcttggc
      781 gatcgggttc atgccggttt acgtcccgcg cgagctgttg catgcgcagg gtgtgctgcc
      841 ggtcggcgtc gtcggcggcg gcgacgaggt cgagatcatc cgcggcgacg catactacca
      901 gtcctacatc tgccacatcc cacgcagcac gatcgagctc ggcctgaacg gcggcctgga
      961 ctgcctcgac gggatgatct tcccggcgat ttgcgacgtg atccgcaacc tgtcgggcgt
     1021 gtggaagctc gagttcccgc acaagctcgc ctactacctg gacctgccgc agaacttcga
     1081 tcgctcgatc ggcggacggt tctaccgcga cgagctgtgc cgtctgtcgg ccgagctgac
     1141 cgagcgtggc gcgcgccagt acgacggcga gctgctgcgc gcgtcgatcg agctgtacaa
     1201 cgagcagcga aggctggtcc aacggctgta cgacctgcga cgcgagcagc cgtggcgggt
     1261 gccgaccgcc gaggcgtacg tgctgctgcg ggcggggctc gtcgtcgcgc ccgaggaatt
     1321 ctcgcgcatg ctggcggact acctccgggc ggcgagtgcg gatgatcggt gcaagccgat
     1381 ggaccaggcg aaagtggtgc tggtcggctc gttctgcgaa cagccaccgc tcggtctgat
     1441 caagacgctc gagcgctccg gctgctacat cgtcgacgac gacctggtgc aagtccatcg
     1501 ttggctgcgc cgggaggtgg cgacggcggg cgacccgctc gatgcgctga ccgccgccct
     1561 gctcgaccac gcaatcgaca gcccgacgcg ctactccccg aacggcgcga acgggcagga
     1621 cctcgtcgaa cgcgtgcacc ggtcgggcgc ggagggggtg ctgttctgcg cgccgagctt
     1681 ctgcgacccc gccctgctcg accagccgat ggcggtcggt gcggtggagc gggccggcat
     1741 tccgtggacc gccttcaagt actcggagaa cggcggtcag ttccaggtga tccgcgagca
     1801 ggccggcacc tttgccgatt cgatcaagct gtggagcgag gtgcatcgat gagcgagccg
     1861 accgtcacgg gaacgcccgc ggcactgaag gacgacagcc agctgcgcca gaagcgaatg
     1921 atcgccgagc actacacccg gctcgcccgc tcgccgcaga cgggagagaa gagcgtctat
     1981 acgttcgtgc ccggcaacct gacggagctg ctgcgcagct tcgacctgct gccgctgctg
     2041 ccggagatca acgccctgca gtcggcgatg cgcggcaagt cgcgcgagta catcgccgcg
     2101 gccgagcgac tcggtcattc ggaggacgtc tgcacctacg tcaagtgcga cgtcggcatg
     2161 ctccgctccg gaaacgtcgg tccgacgggt gaggtgctgc ccgatccggc gatgctgctg
     2221 ctgtcctaca ccgggtgctt caccttcatg aagtggttcg agatcctgcg acgggagtac
     2281 cgttgtccga ccgtgatgct gcacgtgccg taccaggccg acggcgcggt caccgactcg
     2341 atgcgacgct acgtcgtcga tcagctgacc gacgtggtca tcccggcgct cgagaggctg
     2401 tcgggacggc ggtacgacca ggaacgcttg tcggagatgt tggcccgctc ggccctggcc
     2461 gaggacgatc tggtctgggt gctgcgctcg gccgcccggc ggccgtcgcc gatcgacgcc
     2521 tacttcggtg gggtctacta catcggaccg atcttctcgg cctttcgcgg caccgaggag
     2581 gcggtcgact actaccgcgc actgcgcgag gaggtgtcgc gccgagcggc gcgtggcgag
     2641 ggaccggtca cgcccgacgg ggtgatcgac cgggagcgct accgcatcgt cgtcgagggc
     2701 ccgccgaact ggacccactt ccgcgagttc tggaagatgt tcgccgacga gggcgcggtc
     2761 gtcgtcgcat cgacctacac caaggtgggc ggtgtctacg acttcggctt ccgtcacgat
     2821 ccgtcccggc cgctcgagac gttggccgac tactgcatgg gatgctacac gaacctcaac
     2881 ctgccgggcc gcgtcgacat gctgtgccgc tacgtcgagg agtactcggc ggatggtctg
     2941 ttggtcaact cgatcaagag ctgcaattcg ttcagtgccg gccaactgcg catcctgcgc
     3001 gaggtcgagc ggcgtacggg gaagccggcc gggttcatcg aaagcgacct ggtcgacccg
     3061 cgctacttct ccgccgccaa catcaagaac cggctcgagt cgtatttcca gatgatcgag
     3121 cagaaacgga gcgccgcatg aggtactcgg tcggcatcga cctcggctcg acgacgacga
     3181 aagcggtaat cgtcggtgag agcggggcga ttctcggccg cggtatcacc aacagccgga
     3241 gcaactaccg cgtcgcctgc gacgtcgcgc tcggcgaggc actgatcagc gcccgcttca
     3301 cgttggttgc cgacgagtgg cggcgcgccg gcggcgccgg cgcggacgac ggggcgctgc
     3361 tcgggcgtct cgagctcggt tttcgcgaac aacagtaccg tgcgcagctg gcgacgctgg
     3421 ccgaggtgat cgaccgtctg tcgcgatccg tcgcggacgc ggacgcggtt cggccggcgg
     3481 tcgcctggat cctcgaacag atggagcgcg agacgccggg gctgtacgcc accgacggcc
     3541 agcgccggag cgacttcttc cgtgatctcg ccggcgcgcg ctacatgcag ctcggcgagc
     3601 aggtcgcgcg cggccgggag gtcggcttcg atcggctcgc cggactgttc gacaaggcga
     3661 tcctcgaggt cgagaacagc ccggtcgctg ccggtgattt cgccggacac gccggtcggg
     3721 cgctcgagcg gatggccccc gtgccggaga agctggtcgg ggcagtggct cgggcggcgc
     3781 gggcacggct cgagcaagcg gcgagcgtcg gcaccggtta cgggcggcag accctgccgt
     3841 tccccaagga caagatccgg tccgagatcc tgtgtcacgg cctcggcgcg cacgcgatgt
     3901 tccccgcgac ccgcacggta ctcgacatcg gtggccagga caccaaggcg attcaggtcg
     3961 acgagaacgg cgtcgtcacg tcgtttcaaa tgaacgatcg ctgcgccgcc gggtgcggtc
     4021 gctacctcgg ctacatcgcc gacgagatga acctcggagt tcaccaactg ggcccgctgg
     4081 cctgttcggc ccggcgcgcg gtgcggatca actcgacctg caccgtgttc gccggagccg
     4141 agctgcgcga gcgtctgtct ctcggcgaac cgcgcgagga catcctcgcg gggctgcacc
     4201 gcgcgatcat cctgcgagcg atgtcgctgc tggcgcgatc gggcggggtg tacgaccagt
     4261 tcaccttcac cggtggcgtg gcgcggaacc cggcggcggt cggcgcgctc gagcgattgg
     4321 tcgacgagaa ctacggtcgt ctgacgacga acatctcccc cgattcgatc tataccggcg
     4381 cgctgggcgc ggcgttgttc gcccggcgcg gcctcgctcg cgacgggcgt gacgcggctg
     4441 gcggcggagc gcgagcatga cgcgcgagac ggtcggaccg gtcgcgggcg tcgacgtggg
     4501 ctcgtcggcg gtcaaggtcg tggtgctcga ccaaccggcg ggcgccgagc cgtgcacgct
     4561 gttcgccgac agcagccgaa tccgccgccg cgatccgcag cgggtcgcaa acgagctgtt
     4621 ccgtacggcg ctgacgacga tcggactcga acccgagcag ttgacctacg tcgccagcac
     4681 gggcgacggc gagctgctcg actttcgcac cggtcacttc tacggcatga cgacgcacgc
     4741 gcgcggcgga ctgttcctcg accccgaggc gcgggcggtg atcgacatcg gcgcgctgca
     4801 cgctcgcgcg gtgttgatgg acgagcgagc caaggtgctc ggctaccgga tgacgagtca
     4861 gtgtgcgtcg gggtccgggc agtttctcga gaacatctgc cgctacctcg gcatcacgat
     4921 cgaggagatc ggcgagttgt cgacgcgggc cgacgacccc gagccctgct cgtcgatctg
     4981 cgccgtgctg gccgagacgg acgtgatcaa catggtctcg cgcgggattt cgacggccaa
     5041 catcatcaag ggaatccacg tctcgatggc cggccgctac ctgcgcctgt tggcttcggt
     5101 cggcgcgcgc ggggtggtgc tggtcaccgg cggactcgcc ggtgacgccg gtctgctgca
     5161 ggcgctgcgc gagggcgtcg aggcccagaa gagcaagctg ccgatcgaga tccgcgcgca
     5221 cgtccactcg gcgttggccg gagcgtacgg cgcggccttg tggggtgcgt tccgcgcgcg
     5281 gaagctgggc ggtgcgggag ctcgcttcgc agcgggggct caatgaacgc gaccgctcga
     5341 ccacccgcgc cgctttcgct cgagggccgg accggcatcg tcagcggagg agcgggagga
     5401 atcggctcgg ccgtcgtccg cacgttggtc ggcgccggcg cgcgggtcgc gagcgtcgat
     5461 ctgcccggta gggaggcgcc cgccggtgca accgcgatac cgtgcgacct gagcgatgcc
     5521 gccgcgatcg accggatgtt cgcggacttc gacggtcgat tcgaccggct ggacttgctg
     5581 gtgcactgcg ccggcgtgac ccgcgacgcg gtgctgtgga agatgagcgc cgacgactgg
     5641 tcgcgcgtga tgcgcgtcaa tctcgacgcc gcgtttcacc tgctgcgagg tgcggcgccg
     5701 cggcttcgcg ccgccggacg aggcgcggtg gtgctgatct cgtcgatcaa cggcgagcgc
     5761 ggcaagttcg gccaggccaa ctacgcggcg agcaaggccg ggttgatcgg gctcggccgc
     5821 acggcggccc gcgagctcgg ccggttcggc gtgcgggtca acgtcgtcgc gcccggttgg
     5881 gtcgagaccg cgatgaccgc cgcgctaccg gccgaggtca agcgacaggc gatcggcgag
     5941 agcccgttgg gacgcaccgc gacacccgag gacgtcgccg ccgccgtctg gtttctctgc
     6001 gcggagatgt cgcgtcacgt caccgggcag gtgttgcggg tcgacggagg acagctgatg
     6061 gcctgagccg ggccgtccgg aggagacgat gatggaagcg acggacgagc gcgaggatcg
     6121 ggtggtggtt cgcggattgc ggccggccga tctcgacgcg gtgatcgcgc tcgacgcgaa
     6181 gaacgtcggg cgccgccgcg agcagttctt ccggctcaag ctggagcaga acctgagcga
     6241 gacggggatc aaggtctcgc tggccgccga gctggacgcg tgcttcgtcg ggttcctgct
     6301 cgcgcgcgtc tactacggtg agttcggcag cctcgaaccg gtcgccgtgc tcgacacgat
     6361 cgacgtgcat cccgacttcc gcggtcacgg cgtcggttcg gcgctgatgc gccagctgcg
     6421 ggtcaatctc gtcgcgctcg gcgtgagcca gctgcgcacc gaggtcggct gggacagtca
     6481 gcaactgatg cgcttcctgc accgcgaggg gtttcgaccc gccgcccgag tgtgcctcga
     6541 cctgccgctc gagtgggaac gggtcgggcg acgatgttga cgctgagcaa gagcacgcgt
     6601 tacgcgctct acgcgacgat ggagatggcc cgcgccgcgg gcgacggtca cgtcacggcg
     6661 gcggaggtgg ccgggcgcta caagatcccg ccgagcgtac tggccaaggt gttccagcag
     6721 ctggtccgct cggggctggc ccgcggctcg cgcgggtccg gcggcggata ccgcctcgcg
     6781 cgcgcggccg gcgagatcac ggtcctggag gtgatgcggg ccttccaacc gccgaacggc
     6841 gcagtgccga gcgccgccgg cgagccggcg gcggccgcgc cgctgacgcg gttgctcgac
     6901 gaggtcgccg agctggcccg ctgcaccttc gcgtcggtga cgctcgagac gttgacccgc
     6961 acggcccgac ccgtgcaccg cgttccgtca gccggacggt agccgtcgca ccaacacgat
     7021 cgtgatgtcg tcggccggcg ggcgaccgtc ggcgtggcgc cgggccgcgt cgaacagcag
     7081 ccgcaccagc tccgacatcg ggcggtcgtg gtggcgtcgg acgacctccg ccaccccggt
     7141 ctcgccgaac atccgctcgc gcgcgtcggc gtactcgaag atcccgtcgg tcatcacgcc
     7201 gaccacgtcg cccggtgcga ggacgaactc ggccggcttc ggcaccggca gcgacgcgat
     7261 gcagccgagc ggaggcgccg tcgagttccg ccattcgcac tgatcggtcg cggcgtggta
     7321 gtgcagcagc ggaccctgcc cggcggcgtg atagaacagc ttgccggcat cggcgtcgag
     7381 ccggccgagg aacgcggtga ccatccggtt cgccgcgagg tcttcggtga gctggtcgtt
     7441 gacgtgagcc agcgtctgct cgaggctcgc tcccaggcgg ccggcgatgc gcaccatcgc
     7501 ccgcacctgg gtcgcgctca gcgctggacc cactccatgg ccggtggcgt ctccgaccag
     7561 catcaccgcg ccccgcggtc ccgagccgat ccagtcgaag gtgtccccgc cggtctcctc
     7621 cgccggctcg gtcacgccgg ccaggtcgta gccgtcgacc cgcgggagct tgcgcggcag
     7681 aaagccggtc tggatctcgc gcgcgacggc cagctcgcgc tgcatgcgct ccttctccaa
     7741 cagctcctgc gtcatccgca ctcgctcgag cgcgacggca cactgcgcgg ccagcgcgga
     7801 ggcgatccgc tcgtcgcgtt ggctgaacgt gccgcgcttc ttgttcagca attgcagcac
     7861 cccgaccaac cgatcctgct ctgcgatcag cggtaccgcg agcaggcagc gggtgcggta
     7921 accgctctcg cgatcgacgt cgcggttgaa gcgcgcgtcg gcgtagcagt ccggtacgtt
     7981 gatcagccgg cggctctgcg cgcattcgcc ggcgatgccc ttgcccgccg gaatgcgcag
     8041 ctcgcgcacg ccggtgccga cgcggacgac gagctcgtcg gtcgtttcgt ccaacaggaa
     8101 gaccgtcccg cgatcggccg ccagcacggc gcggccggcg tcgatgatcc gcgccaggat
     8161 ctcgtcgagc tcgaacggag ccgccaagcg gcgggttacc tcgaggatgc tctcgagcgt
     8221 cgccgcgtcg agctgtcggc ttctcgccgg acggtcggac acgcctgacc ccttcgtcgc
     8281 cgttccacgc ggcctacttc tcggtgaacc ggatcacgcc gtcccactcc ccggccgtcg
     8341 gctcggcccg ccgccgggcg atcgcgtcca ggtagagctc cgacggcccg tcccgcccac
     8401 cgcaacgctc gcggacctcg cgaaagcctt cggccgcccg ctcgaacccg ccggcgacga
     8461 aatcgccgac ggcgcgctcg aatcggccga tcgtctcggc atcgggacgg gtgaccgcgc
     8521 cattctgccc cagcaattcg tggatcgcca ccggctcctc gcgtccggcg acccgcaccc
     8581 gaccgagcgc ccgcgtgacg aacgtcccgt cggtggcgcc gagcgtctcg gcgctgacca
     8641 gcgcgcgcgt gccgaactgc ttgttcagcg actcgagtcg cgcggccagg ttgaccgtgt
     8701 cgccgatcgc cgtgtagtcg aatcgctggg ccgaaccgag gttgccgacg agcatcgagc
     8761 cggtgtggat accgatccgc gtgatcaact cggagcccgc gccgtcgccc ggcgcctgct
     8821 cggcgcgggc gatctcgatc gccgcgcggc aggcacgggt ggcgtggtcg tcgaacggca
     8881 ccggtgcccc ccagatcgcg aagaccgcgt cgccgatgta cttgatcagg gtcccgccgg
     8941 tgtcgaagat gcgtccggtg acctccgaga agtaccgatt gagcatcgcc gcggtctccc
     9001 gcgcgcccat ccgctcggcg attcgggtga agcctcggat gtcggtgaac accgccgtgg
     9061 cgacgacctc ctcccctccc aggctcaacg cctcggggtt ctcggcgatt cggcggatca
     9121 tctcgggcga gaggtagaag gcgaacgcgc gtcggatctt ctcgcggtcg cgcgcagtgg
     9181 tcacgtagta ccagaccagg ctgacgacgt aggccgccgg cagttggacg accgacggga
     9241 tcacgaccgg caaccacagc ccgcccgcga cgaaggcgaa gtacgcgccg ctccacgcga
     9301 ggagttccag cccgcacagc atcgccgcgc cggcgagcgg ccgcagcacg atgaacaaca
     9361 gcgaagccag cagcgcgagc gccagtacca gcagcgtctc gaccgcccgc agcggcaggc
     9421 ggatctcgct gccgtcgacc aggttggccg cgaccgtcgc gtggatctcg acgccgtagg
     9481 tcaagccgac gtcgccgccg ctgaacggcg tgggaaacga gtccttggcc gccgacggcg
     9541 tcagcgccgc cggctgggac gcgccgacga agacgatgcg atcgcggaag taacccgcgg
     9601 gcaagtactg atccggatcg agcgcctggt acagcgagac cgtcgggatc gtccgcggtg
     9661 gaccgtagta gtcgatcagg cgtgccgcgc cgtcggcgcg tggcgcatcg ccaccggtcg
     9721 ccacccggta ggccgccagc gccagactcg gtcgctcgtc gagtacgagc cacgcgcgtc
     9781 gcacgacacc gtcggcgtcg gtcggcagct cgaccgatgc caccgccgcg gccgactcgg
     9841 cgaacggcgg gtgcggatcg atcctgcgcg tcgagcggaa acgcgggtcc tccacccgct
     9901 cgacggttgc ggcgagcacg acgttgccgg cgtcgaacaa cgcgagctcg agctcgacgt
     9961 cctgcgccgg gtcgcgcggc tcgtcgaaca gcacgtcgac cgcgatcgcg cgcgcccctt
    10021 cgcgcgcgag cacaccgatc aagcgcgcgt gcaccgcgcg cggccacggg tcgagcggat
    10081 cgtggccgag ctcgatcgcg gaggcgtcgt cgatcgcgac cacgcagacg gccggcggcg
    10141 gttgtcgcac gccgcggagc tgcatcagca gatcgaagcc gtagcgggtc tcgagcgcgc
    10201 cggtcaccgg ccagcgcaga caaagccagc cgagcagacc cggcacgagc gccagcagga
    10261 tcaccgcccg gccgcgcgac acgagtgccc tgttcatttc cccggccccc gccgacgccg
    10321 gctgcttcgg ggatcgtccg cggacactag ggcgacgtga agctgagact caacagcacg
    10381 gtgcgcgccg ggatcaccga ctcctcgagc gagctccggt aatagtcgaa gtcttggtcg
    10441 gtcacgttga acacccgcag cgagaccgag ccccaacgtt gcgggaacag atagcccagc
    10501 tcgacgtcgc cgatccaaaa gtcgttctca ttggcggtgc gtacgtccga gctgagatcg
    10561 gtgaactgat cgacggtctg gtcgtagtag gtcgcctgca cgccggcgaa aaaacccatc
    10621 ggtaggaaat agcgcacctg cggccgcacg cggtgggtgc gcatcgagtc ctcgaaacgc
    10681 gacagattgt cgacttgagt gaaatcgaac tcacgctgct cgtaggcgta ctccagactg
    10741 agagcgactc gccttcccgc ggtggcattg agatagacgt tgcccagcca gtcgtcgctg
    10801 gtgcgctcga cgatctgcga cgacggctga gcgaagccgc agccgatcgt cggatcgggg
    10861 ttctcgcacg tcggctcggg aatctcgaga tcgcgtcgca acacgccgag cccgccgaag
    10921 aaccccttgg cgaactgctg gtcgagaccg acgccgtagt tgtcggacga ggtgccggcc
    10981 ggatcgtcga agaactggtt gaagccggcg acctgggtcg gctcgagcgt ctgcagacga
    11041 ccgatcgccg ggttcaaccg actgtacgcc gtcgcacgga tcaccgtcgt cggtgtgccc
    11101 tgaatcgaca gaccgagctt cgggctcagc tggttgtcct cgtacggcac gtcgcccggc
    11161 agcacaccgc tgtcgcgcgg gggaagaaag ccgaccggcg ccgtcacttc ttcgtacgag
    11221 gcacccgcgg tcagctcgac cggacccagc ttgcgcagct tggcgtagag atagccgttc
    11281 gagaattcgt cgtcggccga cgccgaggtg ggctggccga ggaccaccgt gtcggccgtc
    11341 tgctctccgt cgaaatgtcc gacgccggcg gtccacgtaa gccacgtctg ccggcggacg
    11401 tactgcgcct cgatctgcgg gccttcgagc tgggccgatc cggtcggttg accgtcgaag
    11461 cggttgaccg cggtctgccg cgtgtcgttg tagatcagcg acaccgccag atccgcggcc
    11521 ggcgagatga tgcggtggta gcccaggccg acgttggtca actcggtctc gatccgctcg
    11581 atcccgggaa tgaacggcac ggcgcgcagc ggcaggtctc cggtctcgcg cgtgccgtcg
    11641 atgaagctca gctggaactg gtcgcggtag ctcggagcga atgccaggaa accgcgtagc
    11701 gccttgttgt cgacgtcgtt gttgatgcga aacccatcgt cctcgaagct ctcgtaggac
    11761 agagcgccgg cgagccggtc gcggttgaag ctcaccgtgg cctcgccgcc ggacgggttg
    11821 ctgtcgccga tctcgagtgg ggcgagcgcg gaattctcga ggtcggtgtc gacctcgccg
    11881 tagccgatcg tggcgaagcc gcggacgcga ttgcggttga tcaacgacgt gtactcgttg
    11941 aacgacacgc tctcgttgac cacgtccggt cgcaccgcat tgacgttgac cggctggtag
    12001 atccgggctt gcagcgtctc gctgaggaac gccggggcga agccgggcag gcgccggtag
    12061 gtgcccgcga ggaacaggtg ggccgagtgg ttggactggt cgatgtcggc gctgcgccgc
    12121 gcggtaacca tgccgagctg gtcgaagccg aggtcgttgt aggtgccggc gagatcggcc
    12181 gagcgcaccg ccaggtcttc gtcgagcagc aggcgcgagc ggtagaccgc gcgcttgtcg
    12241 ttcaactcga tcgatttctg caggtccttc agcgcttcga cgggacggtt gtaggtgcgc
    12301 ttgaccagcg ctccgtagag gtacggcgtg gggtcggacg gatcgagctg cttggcgatc
    12361 tcgagctcct tctccgccgc gtccttccgt cgttcctcga agtacgcctt gcccaggtac
    12421 gaccgataga gcgaattctc cggatcgagg atcaccgcgg tctgca
//
LOCUS       DASPGN010000394        19897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1711522, whole genome shotgun sequence.
ACCESSION   DASPGN010000394 DASPGN010000000
VERSION     DASPGN010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 19897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19897
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711522"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="VD788_11390"
     CDS             complement(<1..709)
                     /locus_tag="VD788_11390"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HXV76907.1"
                     /translation="MRSFAPLLSSVIPFAICVLVAAAALADDEPPATEWVCPPCGGSC
                     DATIHSAPGRCEQCGMQLVRKQEVRARNVAILVFDGVQIIDYAGPYEVFGQAGFTVFT
                     VSKTGEPITTAMGMRVTPTYALDQAPPAEVIVVPGGHVEDTYVDQEVLDWLRERSTHA
                     AKVMSVCNGAFIMARAGLLDDMRATTFYGLIDDLRTFAPKVEVVADQRFVDNGKVVTT
                     AGLSSGIDGSLHVIGEML"
     gene            complement(706..1704)
                     /locus_tag="VD788_11395"
     CDS             complement(706..1704)
                     /locus_tag="VD788_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HXV76908.1"
                     /translation="MIPDQPTVARRPRVVFLLLPRLHMQDLAGPAQVFYEAAGFGAGY
                     ELVFAATVERVCTAQGLVLADLVPPPEPRPTDRVIVPGIDSAELDRLDAPFDWLRAAL
                     GAGCEVGSICSGAFALARAGLLDGRRCTTHWKIADRLEREYPRARVHDNRLFVRDGRV
                     VTSAGVTAGIDLALSLVREDCGPRTAALVAREMVVYLRREGEGQQLGAFLEHRTHLHP
                     GVHRVQDWLTEHPDRNATLDELGRIAGMSARNLTRVFRRATGITLKEFSGSIKLEVAS
                     RMLERPDLSIDAVAERCGFRDPRQLRRLWKRRHGTSLQGWRALHREKQDRSERNMR"
     gene            complement(1782..3182)
                     /locus_tag="VD788_11400"
     CDS             complement(1782..3182)
                     /locus_tag="VD788_11400"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV76909.1"
                     /translation="MTLRSALQSALAVAAFAAVPASAGERPAPAWAQWRGPDRAAHAL
                     DFDAPDAWPEKLTRVWRVEVGAGVASPVVAAERVCLLTRDGDDETASCRRLEDGSLIW
                     SRSYSSPFYANGGAINPRWYPVSRGRGPFATPVLHDGRLLTLGVDRVLSSFDAATGEL
                     VWQRRFIPVDVPAKPVFVCRPCGCVDDGKKFDSPGICPACGMGLSAEGIETAATTSGG
                     NYYGAASSPLVAGDLGVVHVGNPTRGLLVGFDPTSGEERWRWEGPVVGYSSPVMATIH
                     DERQVVTMTRTAVVGVSAATGRPLWTFAIDNNAHAATPVVLGDLVICSEYRGRTFAVR
                     VAKIETGWEATLAWANPEMTQWFGTPVLDADRLYGFFYSRRGQLAALDARTGETLWTD
                     EGRQGESAAVVAAGNELLALTNDGRLLVFGKAADSHRPIRSYELTDSPAWTYPALVGR
                     DLLIKDEAALTRWSVD"
     gene            3288..6956
                     /locus_tag="VD788_11405"
     CDS             3288..6956
                     /locus_tag="VD788_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXV76910.1"
                     /translation="MSRRRKILLGAVTVPCVLFALLVFVVFADLGFLRGTVERVVGAA
                     IGCELEIGGRFDLRLLGTPTVVAEDVVLVDPARVAAHPLVRIERLVAQLDPVSLLSSP
                     IHVTEVELRGVRIDLDADAGLRPRRGTGRPADERREVDRPPVLQVEVDHATLFDGSLT
                     LDGSSERPWMLEVERLDATAAAGRPASVRVTGRLDGSPIELSAETDPLDRLIVGRDVA
                     YRLTVDLAGTTVRSHGRIGDLAGLVDPTLDVELAGASFDELARLLRLDTSLAGPFDLR
                     AQVRPAGDRVEVRLVATTEPLTASLSGHSVSLLDFAAYEVGGEVRGTNLAAVGALFGR
                     HGLPAATFALAGSVRHDDGTTTLDGVTARIDENTLSVDGSLGPAPARVGTDLEFDGRG
                     PDLSSFSKLTGFDLPRGPYALHGRVVRVDEGYRLERVDLRTAAGAAVVDGVAGNYPEF
                     AGTRLVVGIDVADLSVFSRPARVALPAEPLVARGTVDYDDDRLWVSGLRFELGATRGT
                     IDGAIDGPPTFDGSEVDATIEGPELCGVGRWFGITGLPCRAFRAVGSVRFDDGLDLRS
                     VAIRLEPATGEGGVAELRLDGRVETRPHLLGTDLRLEADGVRLADLTSVVPLPSRPFE
                     VSGRLVLEQAGAARLDGVSGRLGEHRFAVDGVIGLARGLGGTRAKVELAGVDLADAAR
                     LLADSGVDRIPPLPATDYSITGTVERVEDAYRLIGIRGTIGPSLLTLDGSIGRGPDPT
                     VDLTLDFRGPQASLLDVALDARLGPEPFHLSGRIERRESATRFDALSLLLGDSRSRLD
                     GVLGPPPTFVGSDLDFRLAGGSLAVVGSIASLDRLPPVPFEIEGHVEGTTEVFSLAGL
                     RVVVGQTDLAGGLSGDLRGAKPRFDGEFVSNRVDLGPWLPVTEPPAAEVAGAAATSTE
                     NKPGKPRLVSDAPLELDRLRKLNGRLVWTARELVGYREGLGDLRLVMSLDDGRLRIDP
                     LALRGQHGGRFDIRFGIEPFGAGHRAELEANLEQIRLFSVLAEVTAVEQSPPVSLDVR
                     LRGAGRSLHEMLASADGHAVAIVEPGRIESWRYEFLTTDVLAALLDALNPFRKERRST
                     EIECAVVVGRLEHGVLRLDPIAARTDRTTVVGEGRVRFDDERIDLDWAAKPRQGIGLS
                     ATAITNSYIKVSGRLSDPSIDVKPLRAVANTGAAVATMGLSILARGLWDRVTAERKVC
                     ERALEDAARIAPGSHDGG"
     gene            6946..7851
                     /locus_tag="VD788_11410"
     CDS             6946..7851
                     /locus_tag="VD788_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76911.1"
                     /translation="MAGRTAEEEVRTTSGRRRAAKRLIAAIWVVVCAASLAIRGQPTG
                     EPAPVETQRELARILERLANVAELYLDNVLRFTCDETIHFTGRGAPVVHRFQYLYRYS
                     EDDASLRDFRRTRSGKTALGSLAGFGLPAYLERAYSWVFIFLREVQPRYRFRLDGEDD
                     VLGRPGHRVSFAAIPPYEPGVNDWFGTAWVDRDGYQLLVVEAWRLDDRHARRLIEREA
                     TVERGEGKASFETSIYRTEFDMVKNGMRFPGRVTIERTAHRFQGGSTRARVVPLWRVD
                     QIYKRYRFFGVRTEQEIQRLVQRSE"
     gene            7904..8725
                     /locus_tag="VD788_11415"
     CDS             7904..8725
                     /locus_tag="VD788_11415"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HXV76912.1"
                     /translation="MIDLEQRVREFHRLHESGCFVMPNPWDVGSARLLEQLGFQALAT
                     TSAGFAWSLGRPDNGATLDEVLAHLRAVAGAVAVPVSADFEGGFAVAPAEVARHVAAA
                     STTGIAGLSIEDSTGDPAAPLFDRALATERIRAARRAIDDGCAGIVLTARSEGFICGR
                     PDLAETLRRLEAFAEAGADCLYAPGLPTRDEIAAVVAAVAPKPVNVLASGSLGSVSEL
                     AALGVRRISVGGALARAALTGLLDAAGEIAQRGTFDGLASARPFVRINGAFLVER"
     gene            complement(8735..9157)
                     /gene="mscL"
                     /locus_tag="VD788_11420"
     CDS             complement(8735..9157)
                     /gene="mscL"
                     /locus_tag="VD788_11420"
                     /inference="COORDINATES: protein motif:HMM:NF001843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXV76913.1"
                     /translation="MGMIKELKEFAIKGNVVDMAVGIIIGGAFGTIVKSLVSDVIMPP
                     IGLLLGGVDFSNLFLVLGQGSYDSLAAATESGAATLNYGVFLNNVVSFLIVAFAVFLL
                     VKSINTLKRKEQATPAAPPAPPPDVVLLTEIRDLLKRS"
     gene            9300..10052
                     /locus_tag="VD788_11425"
     CDS             9300..10052
                     /locus_tag="VD788_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76914.1"
                     /translation="MGKPSKFVLAGGVVVLIAAGIAVWLLLGNLDRIVKRAVERVGSE
                     TTGTRVALDDVLISIRSTEGALDGLTIANPPGYETTHALELERVRLALDLSSLRTDEV
                     VLREVAVDGAHVVFEQKGQTSNLRTLLDHVERAGRESGDPRTAAGDELRLVVEKFTFS
                     GGKVTLIHDRLDEDLTFEIPEVVVRDIGRVGAGETIEDVARQLLEPVLERTIDSAKDE
                     VEQMVKQRLEQEVESRKQQALESLKDRLLDKD"
     gene            10130..11323
                     /locus_tag="VD788_11430"
     CDS             10130..11323
                     /locus_tag="VD788_11430"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXV76915.1"
                     /translation="MTHHGRRNGRLESRRLSSLVLSATVLLQVAPASASAIPRFVFVD
                     GSDRFAERIACRSWGSRPGFALTVLHRGRSVLSEQCGLADLGRGVPVDGDTRFRATPV
                     GKTLISAAALLLHEERALSLDERLSSFFPGLPEWAQRIRVLDVLNHTAGLPEFRDETG
                     LPDSIDGEWVLAFLARRPRPAFEPGSSFAESESGYLVLTELIERTSGLRWIDFVEDRI
                     LRPLGMHETRLVDGPAAEVPARAIGYRSTGDGFEIDRSPSDTPIGSFEVFTTVGDLTR
                     WYSALAEHELLTPRSTRMMFHFPLTSSGSVSSVAMGWVDRTVPVDARRHAGLRGFATG
                     DSSMELAFYPDEELCVILLGNADRLPAFLDDALSHYLIDVPRRKAAYPADDPFGRERG
                     ASHRR"
     gene            11371..12576
                     /locus_tag="VD788_11435"
     CDS             11371..12576
                     /locus_tag="VD788_11435"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HXV76916.1"
                     /translation="MRSLISLVVAVSSIASGCSPGALPPLEPRVADPPAADGSVASDV
                     TTGADGRVYLSWIEPAGGETHRLRFSTWDGDGWSAPRTVAAGDGWFVNWADFPSMAAL
                     ADGTLAAHWLVRSDESSYAYDVLLAVSSDGGASWSEPTAVHADRTQTEHGFVSLLPAN
                     GDEFHVIWLDGRELAGADHGGGAMTLRAARFERNGLRSGPESLIDSRVCDCCQTDAVL
                     AAGGTPVVVYRDRTDDEVRDISIVRREDDAWSEPVAVHHDDWRMPACPVNGPAVDHAS
                     ARLAAAWFTAPDGSGRVQVAFSDDGGRTFGPPVRLGGDRAVGRVDLVMLDDGSALVSW
                     LEESRLSLQRVGPNGTRGPIHAVARTSNERAAGFPRMTRAGELLFLTWTEPAEPSRVR
                     AAVVALADL"
     gene            12765..12965
                     /locus_tag="VD788_11440"
     CDS             12765..12965
                     /locus_tag="VD788_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76917.1"
                     /translation="MARRSRTSILKRQRELRKAEKAAQKRARRHGVSLDPMKEPVPTV
                     SLADLLRAGSDRTNQEPDDDGS"
     gene            complement(13136..14038)
                     /locus_tag="VD788_11445"
     CDS             complement(13136..14038)
                     /locus_tag="VD788_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV76918.1"
                     /translation="MQYSERTYPGNSTTDRSYLPGLPTAARLVVRLAATAAPAPIASA
                     ALGLFLTPPRRQVPERETRWTWGTVPDEFHAAGRRLHGWSIGRGPTVLLVHGWGGRGS
                     QLGSFVEPLVERGFRVVGYDGPGHGRSAGRRSSLPELAEAVRTVGAASGPLVAVIAHS
                     LGAAATTVALDGGLDVGRVAYLAAPARLRYFIETFCAALGLPQTVERRMVRRIERRFS
                     IRLAALDGTAIAYRRALPPALLVHDRADRDVPFAQSRELAEAWPAASLIATGGLGHRG
                     ILRDRAIVDRVVAFVSGDGATGGR"
     gene            complement(14059..14652)
                     /locus_tag="VD788_11450"
     CDS             complement(14059..14652)
                     /locus_tag="VD788_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXV76919.1"
                     /translation="MAIKGEQTRAAILDRAIRLSSTVGLESLSLAVLAREVGLSKSGL
                     FAHFRSKEDLQLQLIERARARFVERVVAPALKRPRGEPRFRTMFEKWLEWDSGPDLPG
                     GCVFMTFAHELDDRPGPPRDALVATLREWVETLKTAARLAIAEGHFRANVDVDELVFE
                     AYGIVLAYNHFHRTLGIERAERRARRAFDALIDRARR"
     gene            14733..16052
                     /locus_tag="VD788_11455"
     CDS             14733..16052
                     /locus_tag="VD788_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76920.1"
                     /translation="MADNIETHKRALQVNLDRRTFGTFAEIGAGQEVARWFLQVGGAA
                     GSVAKTISAYDMTFSDAIYGRGSRYVSRERLLSMLDHEYALLLERLAADRGTTTQFFV
                     FADTVEARNYSGTNDCHGWLGVRFQDEPGGPPSDVRLHVNLRDPTNLQQQRALGILGV
                     NLIFAALYRRDSAATLLDALFTDLSLERIEIDVVELAGPAVAPLDTARLGQRLVRGGL
                     AQAVLLRNDGTLVQPSSLLRKRPLVVERGLFPTVAAFHRQMLDEACAELKRSEKPQRD
                     PLPVPELSVLPVAGREAPDEAETHRRLHELLALGGPVLLTRFPQVYHLTSYLRRYTRE
                     PLRFVFGASTLVQVLHDAHYADLMGGVLEALGRLLADNVKIYLYPMQADVFREIVERH
                     GGASRVAPPASQWVTAETIEFEPPIGHLFRYLREMGWVVGLDPPRAG"
     gene            complement(16062..16739)
                     /locus_tag="VD788_11460"
     CDS             complement(16062..16739)
                     /locus_tag="VD788_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76921.1"
                     /translation="MFARKAGWSWLSVVLLAGLPVAAEEWTLKSVLKQVDRQTKEARW
                     VTAEIAWNEFLGSAIDHDGDGKVWIDLAGRMRAEVGGSNPRTLLLSPAYVQLYKPLEQ
                     VAEVYETLFQPDLLVQYAMLGFAPRGSKLKQDYDVALVKRDEVDGRNTFLLELTPRST
                     ETAASVPVVMVWIDETTWLPARQLIRQSNGGLQVSVSYRHLTVEPSLPEQTFRADWPA
                     GTTVIGK"
     gene            16831..18726
                     /locus_tag="VD788_11465"
     CDS             16831..18726
                     /locus_tag="VD788_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HXV76922.1"
                     /translation="MGNRSLRCRLVAAGLVSASLVGGAARAGWASWDDEDRRRWLEHH
                     AKVEKRLWVPMRDGVRLSTDLYLPADADGPFPVIFWRTPYNFSELDGMLLSFAHESVA
                     RGYAFVLQNERGKFFSEGEWVLLGLPRTDGYDALSWIAEQPWSNGKVGTLGCSSPAEW
                     QLALAAMDHPAHAAMVPMAPGAGIGRVGEFWEQGNWYRGGAEQMFYLPWLYEVQDTQR
                     PRLPDGLSREDLVRLSSYYDLAPEMPEIDWKKKIRILPVADVMERVDGPRGIYKEYFA
                     RRPDDPAWFDSGLWHDDEDFAVPSLWLFSWYDISTTPNLALVDHIVRNAGDAEVRDSQ
                     FVIMAPVEHCAFFRSASPYTIGERELGDIDLDYEERIYAWFDRWLKGEDNGVTRSMPK
                     VEYWTFGVNEWRKAERWPPAGAETIPFYLASDGDANSLFGDGRLEREPPAENRPDRFT
                     YDPTVPVPSLGGGVCCIGGTIEPGAFDQRAIEARADVLVYTSRPLERDLDATGPVRVT
                     LFVSSDAPDTDFTVKLVDVAPDGTAYNLDETIQRVRYRAGYDREVLMKPGEVYELAVS
                     PMSTSNVFLAGHRIRIEVSSSNFPRFARNLNTGGPNHNESEPAVARNVVHHSKRYPSR
                     IELTVLP"
     gene            complement(18754..>19897)
                     /locus_tag="VD788_11470"
     CDS             complement(18754..>19897)
                     /locus_tag="VD788_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76923.1"
                     /translation="LTVLLAWLAIAAAWPGPTGSWSRGRLAAVVGIALCSVFCKESWV
                     ILPGLVVGFELFIARRRPRRALRTAALLAIPAAAYVVAYPWLFPGRGGYYEAGSWVLA
                     KLPHYWAGFMMLTTVESTRAAFGIAELAALGAIAGCAMIGWRQRSHLVGLGFVFFLCA
                     MAPVLFIPFNPTRYTTAPLVGFVLVMTGTVDALCRWSPVRRRRLALVAAAAGALALFG
                     AGVLLLRGDLADMRRLRASHDELVDEIRAFAPRLPTDRPILCVRMDRVDPLGRLDREG
                     ALGLPKLYFKRTVTPYGLADWAQLFTYARAEYGGEIYADLPSPRSAGEGYAIVAHFAG
                     RFALIEPRADSLTAEMAELGRAGYASRLVGPWAPARGTLVARDSHE"
BASE COUNT         3033 a         6894 c         6978 g         2992 t
ORIGIN      
        1 ccagcatttc gccgatcacg tgcagcgaac cgtcgatccc ggacgacagc ccggccgtcg
       61 tcacgacctt gccgttgtcg acgaagcgct ggtcggcgac gacctcgacc ttcggcgcga
      121 acgtgcgaag gtcgtcgatc agcccgtaga acgtcgtcgc gcgcatgtcg tcgagcagac
      181 cggcgcgggc catgatgaac gcgccgttgc agaccgacat caccttggcc gcgtgtgtcg
      241 atcgctcgcg gagccagtcg agcacttcct ggtcgacgta ggtatcctcg acgtggcccc
      301 cgggaacgac gatcacctcc gccggtggtg cctgatccaa cgcataggtc ggtgtgaccc
      361 gcatgcccat cgccgtcgtg atcggctcgc ccgtcttcga cacggtgaag acggtgaacc
      421 cggcctggcc gaagacctcg taagggccgg cgtaatcgat gatctgcacg ccgtcgaaga
      481 ccaggatcgc gacgttgcgc gcccgcacct cctgcttgcg cacgagctgc atgccgcact
      541 gctcgcaccg ccccggagcg ctgtggatcg tcgcatcgca ggacccgccg cacggcgggc
      601 agacccattc ggtcgccggc ggctcgtcgt cggccagcgc ggcggcggcg accaggacgc
      661 agatcgcaaa cgggatcacg gacgacaaca ggggtgcgaa gctcctcatc gcatgttcct
      721 ttccgatcgg tcttgttttt cccggtgcag ggcacgccag ccttgcaagc tcgtcccgtg
      781 acgccgtttc cacagcctgc gcagctgccg cgggtcgcgg aaaccgcagc gctcggcgac
      841 cgcgtcgatc gagagatcgg gccgctcgag catccggctc gccacctcga gcttgatcga
      901 ccccgagaac tccttgagcg tgatgcccgt cgcacggcga aagacgcgcg tcaggttgcg
      961 cgcgctcatc cctgcgatgc gtcccagctc gtcgagggtc gcgttgcggt cggggtgctc
     1021 ggtcaaccaa tcctgcaccc ggtgcacacc ggggtgcagg tgtgtgcggt gctcgaggaa
     1081 tgcgccgagc tgctggccct cgccctcgcg acgcaggtag accaccattt ctcgcgcgac
     1141 gagcgcggcg gtgcgcggtc cacaatcctc gcggaccaac gacagcgcga gatcgatgcc
     1201 ggccgtcacg ccggcgctgg tgacgacgcg gccgtccctg acgaacagac ggttgtcgtg
     1261 aacccgggcg cgcgggtact cgcgctcgag ccggtcggcg atcttccaat gggtcgtgca
     1321 gcgtcgcccg tcgagcaagc cggcacgggc cagcgcgaac gcgccgctac agatcgaccc
     1381 gacctcgcaa cccgccccga gcgccgcgcg caaccagtcg aacggcgcgt cgagccggtc
     1441 gagctcggcg gaatcgatcc cgggtacgat gacgcggtcg gtcgggcgcg gctcgggcgg
     1501 gggtacgaga tcggccagca cgagcccctg ggccgtgcac actcgctcga ccgtcgcggc
     1561 gaagaccagc tcgtagccgg cgccgaagcc ggcggcttcg tagaacacct gcgccgggcc
     1621 ggccagatcc tgcatgtgca gccgcggcag cagcaggaaa acgacacgcg gacgacgtgc
     1681 gacggtcggt tggtctggga tcatacagcc aatactacga tcaactaagt cgttgtttgt
     1741 gaaggaataa ggacaaatgt cggacggatc gggccaaccg gtcagtcgac gctccatcgc
     1801 gtcagggcgg cctcgtcctt gatcagcagg tcgcgcccga cgagcgccgg ataggtccac
     1861 gccggcgagt cggtcagctc gtagctgcgg atcggccgat gggagtcggc ggccttgccg
     1921 aagacgagca atctcccgtc gttggtcaac gccaagagct cgttgccggc ggccacgacg
     1981 gccgccgatt ccccctgccg gccctcgtcg gtccacagcg tctcgccggt gcgggcatcg
     2041 agcgctgcga gctgccccct gcgcgagtag aagaagccgt acagacggtc ggcgtcgagc
     2101 accggggtgc cgaaccactg cgtcatctcc ggattggccc aggccagcgt ggcctcccaa
     2161 cccgtctcga tcttggccac gcgcacggcg aaggtacggc cgcggtactc cgagcagatc
     2221 accaggtcgc cgagcacgac cggggtcgcc gcgtgggcgt tgttgtcgat cgcgaaggtc
     2281 cacaacggtc ggcccgtcgc ggccgaaact ccgactaccg cggtgcgcgt catcgtgacg
     2341 acctgtcgct cgtcgtggat cgtcgccatc accggcgacg agtagccgac gaccggtccc
     2401 tcccagcgcc agcgctcctc gccgctcgtc gggtcgaagc cgacgagcag gccacgcgtc
     2461 gggttgccga cgtggaccac cccgagatcg ccggcgacga gcggcgacga ggccgcgccg
     2521 tagtagtttc cgccgctcgt cgtcgcggcc gtctcgatgc cctccgcact caatcccatg
     2581 ccgcacgccg ggcagatgcc cggcgagtcg aacttcttgc cgtcgtcgac gcaaccgcac
     2641 ggtcggcaga cgaagaccgg cttcgccggg acgtcgaccg ggatgaaccg gcgttgccag
     2701 acgagctctc cggtcgcggc gtcgaagctc gacaggacgc ggtcgacacc caaggtcaac
     2761 aaccggccgt cgtgcagcac cggcgtggcg aacggtcccc tccctcggga aaccgggtac
     2821 caccgcggat tgatcgcgcc gccgttggcg tagaacggtg aggagtagct tcggctccag
     2881 atcaacgagc cgtcctcgag acgccgacag gacgccgtct cgtcgtcgcc gtcgcgcgtc
     2941 agcaggcaga cgcgctcggc ggcgacgacg ggagaagcga cgcccgcgcc gacctcgacc
     3001 cgccagacac gcgtcagttt ctcgggccag gcatcgggcg catcgaagtc gagcgcatgc
     3061 gccgcgcgat ccgggccgcg ccactgcgcc caggcgggcg ccggacgctc gccggcgctc
     3121 gccggtacgg cagcgaacgc ggcaacggca agggccgact ggagcgccga gcgaagcgtc
     3181 atcgagacac agtgtagagc gacttcgacg agctgcccaa cacagcggcc cgcccggccg
     3241 ccgatataat cgcggtgcgc gtgaaagccg acaaccagtc tcaccggatg tcgcggcgac
     3301 ggaagatcct gctcggcgcc gtcacggttc cgtgcgtgct gttcgccctg ctcgtgttcg
     3361 tcgtgttcgc cgacctcggc ttcctccgtg gaacggtcga gcgcgtcgtc ggcgcggcga
     3421 tcggttgcga gctcgagatc ggcggacggt tcgacctgcg cctgctcggc acgccgacag
     3481 tcgtcgccga ggacgtggtg ctggtcgatc cggctcgggt cgcggctcac ccgctggtcc
     3541 gcatcgagcg actcgtcgcg cagctcgatc ccgtctcgct gctgtcgtcg ccgatccacg
     3601 tcacggaggt cgagctgcgg ggggtgcgaa tcgacctcga tgccgacgcc ggcttacgac
     3661 cgcgccgggg aaccggtcgg cccgccgacg agcgccgcga ggtcgatcgg cccccggtgc
     3721 tccaggtcga ggtcgaccac gccaccctgt tcgacggcag cttgacgctc gacggttcgt
     3781 cggagcgacc ctggatgctc gaggtggagc ggctcgacgc caccgcggcg gccgggagac
     3841 cggcgagcgt gcgggtcacc ggcaggctcg acgggtcgcc gatcgagctc tcggccgaga
     3901 ccgatccgct cgaccggctg atcgtcgggc gtgacgtcgc ctatcgactg accgtcgacc
     3961 tggcgggcac caccgttcgg tcgcacggac gaatcggcga tctcgcgggc ctcgtcgacc
     4021 ccacgctcga cgtcgagctg gcgggtgcct cgttcgacga gctcgcccgc ctgctgcgtc
     4081 tcgacacctc cctcgccggg ccgttcgacc tccgcgcgca ggtacgaccg gcgggtgatc
     4141 gggtcgaggt gcgtctcgtg gcgacgaccg agcccctcac cgcgagcctg agcggccaca
     4201 gcgtgtcgct gctcgacttc gccgcgtacg aagtcggcgg ggaggttcgc gggacgaatc
     4261 tcgcggccgt cggcgcgctg ttcggccggc acgggctgcc ggcagcgacg ttcgcgctcg
     4321 ccggctcggt ccgccacgac gacggaacca cgacgctcga cggggtgacg gcccgaatcg
     4381 acgagaacac cctgtcggtc gacggctcgc tgggccccgc gccggccaga gtcgggaccg
     4441 atctcgagtt cgacggccgg ggtcccgatc tttcgtcgtt ctcgaagctg accggcttcg
     4501 acttgccgcg cggcccgtac gcgcttcacg gacgggtcgt gcgtgtcgac gagggatacc
     4561 gcctcgaacg cgtcgacctt cgcaccgccg ccggcgccgc cgtcgtcgac ggggtcgcgg
     4621 ggaactatcc ggagttcgcc ggcactcgcc tcgtcgtcgg catcgacgtc gccgacctgt
     4681 cggtcttctc tcggcccgca cgggtcgcgc tgccggcgga gccgctcgtc gctcgcggaa
     4741 ccgtcgatta cgacgacgac cgcctgtggg tgagcgggct tcgcttcgag ctgggcgcga
     4801 cccgtggaac gatcgacggg gcgatcgacg gtccaccgac cttcgacggc tccgaggtcg
     4861 acgcgacgat cgagggtccc gagctgtgcg gcgtcggccg gtggttcggc atcacggggc
     4921 tgccgtgccg ggctttccgg gcggtgggaa gtgtgcgatt cgacgacggc ttggacctgc
     4981 ggtcggtcgc gatccgcctc gagccggcga cgggggaagg cggcgtcgcc gagctgcggc
     5041 tcgacggtcg agtcgagacc cgcccgcacc ttctcggcac ggatttgcgg ctcgaggccg
     5101 acggcgtgcg gctcgcggat ctcacttctg tcgtcccgct cccctcccga cctttcgagg
     5161 tgtccgggcg gctggtcctc gagcaggccg gagcggccag gctcgacggg gtgagcggga
     5221 ggctcggcga gcatcggttc gccgtcgacg gcgtgatcgg actggcgcgc gggctcggcg
     5281 gaacccgggc gaaggtcgag ctcgcgggag tcgatctcgc cgacgccgct cggctgctgg
     5341 ccgatagcgg ggtcgatcgc atccccccgc ttcccgcgac cgactactcg atcacgggca
     5401 ccgtcgaacg ggtcgaggac gcgtatcgtc tgatcgggat ccgggggacg atcggaccct
     5461 cacttctgac gctcgacggt tcgatcggtc ggggacccga tccgaccgtc gatctcacgc
     5521 tcgacttccg cgggccgcag gcatccctgc tcgacgttgc gctcgacgcc cggttgggac
     5581 cggagccgtt ccacctgtcg gggcggatcg agcgacgcga gagcgcgacg cgattcgacg
     5641 cgctgagcct cctgctcggt gactcccggt cgcgcctgga cggcgtgctg gggccgccgc
     5701 cgaccttcgt cggatccgat ctcgacttcc gcctcgccgg cggcagtctc gccgtcgtcg
     5761 gctcgatcgc gtcgctcgat cgcctgccgc cggtaccgtt cgagatcgag ggtcacgtcg
     5821 agggcacgac ggaggtgttc tcgctcgccg ggctgcgcgt cgtcgtcgga cagaccgacc
     5881 tcgccggtgg gctgtccggt gatctccgcg gcgccaagcc gcgcttcgac ggcgagttcg
     5941 tctcgaaccg cgtcgatctc ggaccgtggc ttcccgtgac cgagccgccg gcggccgagg
     6001 tggcgggcgc ggccgcgacc tcgaccgaga acaagccggg caagccgcgg ctcgtatccg
     6061 acgccccgct cgaactcgat cggctgcgga agctcaacgg gcggttggtc tggaccgcgc
     6121 gcgagctggt cggctaccgc gaggggctgg gcgacctccg actggtgatg agcctcgacg
     6181 acggccggct caggatcgac ccgctggcgt tacgcggaca gcacggcggg cggttcgaca
     6241 tccgcttcgg catcgagccg ttcggtgcgg gccatcgcgc cgagctcgag gcgaatctcg
     6301 agcagatccg tctgttctcc gtcttggccg aggtcaccgc ggtcgaacag tctccgccgg
     6361 tgagtctcga cgtgcggctc cgcggcgcgg gacgctcgct gcacgagatg ctggcctcgg
     6421 ccgacgggca tgccgtcgcg atcgtcgagc cgggccgcat cgagagctgg aggtacgagt
     6481 tcttgacgac ggacgtgttg gccgcgctgc tcgacgcgct caacccgttc cgcaaggaac
     6541 gcaggtcgac cgagatcgag tgcgcggtcg tggtcggtcg cttggagcac ggcgtgctgc
     6601 gtctcgatcc gatcgccgca cgtacggaca ggacgacggt cgtcggcgag ggccgggtgc
     6661 gattcgacga cgagagaatc gacctcgact gggcggccaa gccacgccag ggcatcggtc
     6721 tgagcgcgac cgcgatcacc aactcctaca tcaaggtgag cggccgtctg tccgacccga
     6781 gtatcgacgt caagccgtta cgcgcagtcg ccaacaccgg cgcggcggtg gcgacgatgg
     6841 ggctgtcgat tctggcccgt gggctgtggg atcgcgtcac cgccgaaagg aaggtctgcg
     6901 agcgggcgct agaggatgcc gcgcggatag cgcccgggag ccacgatggc gggtagaacc
     6961 gccgaagagg aagtgcggac gacgagcggt cgacgacgag cggccaagcg gctcatcgcc
     7021 gcgatctggg tcgtcgtctg tgccgctagt ctcgcgatcc gcggccaacc gaccggggag
     7081 ccggccccgg tcgaaacgca gcgcgagctg gcgcggatcc tcgagcggct ggccaacgtg
     7141 gccgagctgt acctcgacaa cgtgctgcgc ttcacctgtg acgagacgat ccacttcacc
     7201 ggtcgcgggg ctcccgtcgt ccaccgattc cagtatctct accgttactc cgaggacgac
     7261 gcgtcgctgc gcgactttcg ccggacccgc tcgggcaaaa ccgcgctggg cagcctcgcc
     7321 ggattcggct tgccggccta cctcgaacgt gcctactcct gggtcttcat cttcctgcgc
     7381 gaagtgcagc cgcggtaccg tttccgactc gacggcgagg acgacgtcct cggacggccc
     7441 ggccaccgcg tgtcgttcgc ggcgatccca ccgtacgaac ccggtgtcaa cgattggttc
     7501 ggaaccgcgt gggtcgatcg cgacggctat cagctactgg tggtcgaggc ttggcgactc
     7561 gacgatcggc acgcacgccg gctgatcgaa cgcgaggcga ccgtcgagcg cggggagggc
     7621 aaggcatcgt tcgaaacgtc gatctaccga accgagttcg acatggtcaa gaacggcatg
     7681 cgttttccgg gacgggtgac gatcgagcgt acggcacatc gcttccaagg aggatcgacc
     7741 cgcgctcgcg tcgtaccgct ctggcgcgtc gaccagatct acaagcggta ccggttcttc
     7801 ggcgtgcgga ccgaacagga gatccagagg ctcgtgcagc gatcggaatg aggacgaccc
     7861 gccgtcgcgg gtataatgcc ggccgatccg gggcttcggc gtgatgatcg acctcgagca
     7921 acgtgttcgc gagttccaca ggctccacga atcgggctgc ttcgtcatgc cgaacccgtg
     7981 ggacgtcggc agcgcgcggc tgctcgagca actggggttc caggcgctcg cgacgacgag
     8041 cgccggcttc gcttggtccc tcggccgccc ggacaacggc gccaccctcg acgaggtgct
     8101 ggctcatctc cgggcggtcg ccggggcggt cgccgtaccg gtcagcgccg atttcgaggg
     8161 cggcttcgcc gtcgcgcccg cggaggtcgc gagacacgtc gccgccgcgt cgacgacggg
     8221 aatcgccggg ctgtcgatcg aagattcgac cggcgacccg gccgccccgc tgttcgaccg
     8281 cgcgttggcg acggagcgga tccgcgccgc gagacgggcg atcgacgacg gttgcgcggg
     8341 catcgtgctg accgcccggt ccgagggctt catttgcggc agaccggatc tggccgagac
     8401 gctgcgccga ctcgaggcgt tcgccgaggc cggggccgac tgcctctacg ctcccggtct
     8461 gcccacgcgt gacgagatcg cggcggtcgt cgcggccgtc gcgcccaagc cggtcaacgt
     8521 gctggcgagc ggcagcctcg gtagcgtatc cgagctggcc gcgctcggcg tgcggcgcat
     8581 cagcgtcggc ggcgcgctcg cccgtgccgc gctgaccgga ttgctcgacg ccgccggcga
     8641 gatcgcccag cggggtacgt tcgacggcct ggcctccgcg cggccgttcg ttcgcatcaa
     8701 cggcgcgttt ctcgtcgaac gatagccccg tcgatcacga tcgcttcagc aagtcgcgga
     8761 tctcggtcag cagcacgacg tccggcggcg gtgccggcgg cgcggcgggc gtagcctgct
     8821 ccttccgctt caacgtgttg atcgatttca ccagcaggaa gacggcgaac gccacgatga
     8881 gaaagctgac gacgttgttg aggaaaacgc cgtagttgag tgtggcggcc ccggactcgg
     8941 tggcggccgc cagcgagtcg taagacccct gacccaggac gagaaacagg ttgctgaaat
     9001 cgacgccgcc gagcagcagg ccgatcgggg gcatgatcac gtccgacacc agcgacttga
     9061 cgatggtgcc gaaggcgccg ccgatgatga tcccgaccgc catgtcgacg acgtttccct
     9121 tgatcgcgaa ctcctttaac tccttgatca ttcccatctc gactcctccg ccgtggggcc
     9181 ggtgcgctcg cggtacggcc cgggggctcg cccgcgattc ttgcacggct cgcccgcaac
     9241 gcgtcggggt ttctgcacgc gaccgccggc gatatgatgc gtgccgaagg aggtggcgcg
     9301 tgggcaagcc gtcgaagttc gtgctggcgg gcggcgtcgt cgtgctgatc gccgcgggga
     9361 tcgcggtctg gctcctgctc ggcaacctcg atcggatcgt caagcgcgcc gtcgagcgtg
     9421 tcggcagcga gacgacggga acccgcgtcg cgctcgacga cgtactgatc tcgattcgat
     9481 cgaccgaagg ggcgctcgac gggctgacga tcgccaaccc gccgggctac gagacgaccc
     9541 acgcgctcga actggagcgc gtgcggttgg cgctcgacct gtcctcgttg cgtacggacg
     9601 aggtcgtgtt gcgcgaggtg gccgtcgacg gtgcgcacgt cgtcttcgag cagaagggcc
     9661 agacgagcaa cctgcgaacg ctgctcgatc acgtcgagcg ggcgggacgg gaatcgggcg
     9721 acccgcgcac cgcggccggg gacgagctgc ggctcgtcgt cgagaagttc accttcagcg
     9781 gcggaaaggt cacgttgatc cacgaccggc tcgacgagga tctgacgttc gagatcccgg
     9841 aggtcgtcgt ccgcgacatc ggtcgcgtcg gcgccggtga aacgatcgag gacgtggcgc
     9901 gccagctgct cgaacccgtg ctcgagcgga cgatcgattc ggccaaggac gaggtcgagc
     9961 agatggtcaa gcaacggctc gaacaggagg tcgagagccg caagcagcag gcgctcgaat
    10021 cgctgaagga ccggctgctg gacaaggact gagtccgccc gttccatatt tctggcggca
    10081 atgaccggtc gcggcgaaac cgccgctgcg gtcgttcgta gcaacagtcg tgacacacca
    10141 tgggagaagg aacggccgcc ttgagtcgag gcggctgtcg tcgctcgtcc tgtcggcgac
    10201 ggtgctgctg caggtcgctc cggcttccgc ctcggccatc ccgcgcttcg tcttcgtcga
    10261 cggcagcgac cggttcgccg agcgcatcgc ctgccgctcg tggggctccc ggccgggctt
    10321 cgcgttgacg gtgctccacc ggggccgctc ggtgctgagc gagcaatgcg gtctggccga
    10381 cctcggccgc ggcgtgccgg tcgacggcga tacgcgcttc cgcgcgaccc ccgtcggcaa
    10441 gacgttgatc agcgcggcgg cgctgttgct tcacgaggaa cgggccctgt cgctcgacga
    10501 gcggctgtcg tcgttcttcc cggggctgcc cgagtgggcg cagcggatcc gcgtgctcga
    10561 cgtgttgaac cacacggccg ggttgccgga gttccgtgac gagaccggtt tgcctgactc
    10621 gatcgacggc gagtgggtgc tcgcctttct cgcgcgccgg ccacgaccgg cgttcgaacc
    10681 cggctcgagc ttcgccgaga gcgaatcggg ctacctcgtg ttgaccgagc tgatcgaacg
    10741 cacctccggg ttgcgctgga tcgacttcgt cgaggaccgt atcctgcggc cgctcggcat
    10801 gcacgagaca cggctggtcg acggaccggc ggcggaggtg cccgcacgcg cgatcggcta
    10861 tcgatcgacg ggtgacggct tcgagatcga tcggtcgccg tcggacaccc cgatcggctc
    10921 gttcgaggtg ttcaccacgg tcggcgatct gacgcgctgg tacagcgcgc tggccgaaca
    10981 cgagctgctg accccgcgat cgacgagaat gatgttccac ttcccgttga cgagctcggg
    11041 ctcggtcagc tccgtcgcga tgggttgggt cgaccgcacc gtaccggtcg acgcgcgacg
    11101 acacgccgga ctgcgcgggt tcgccaccgg cgactcgtcg atggagctgg cgttctatcc
    11161 cgacgaggag ctgtgcgtga tcctgctggg caacgccgac cgcctcccgg cgttcctcga
    11221 cgatgcgctg tcccactacc tgatcgacgt cccgcgtcgc aaggccgcct accccgccga
    11281 cgacccgttc ggccgcgagc gcggagcgag ccaccgtcgg taacgcctcg cgcgaggtta
    11341 gaatccccgg acgaaagggg gcgcggcctc gtgcgttcgc tgatctcgct ggtggtggcg
    11401 gtgtcgtcga tcgcgagcgg atgctcgccc ggcgccctgc cgccgctcga gccccgcgtc
    11461 gccgatccac cggcggccga cggcagcgtc gcctccgacg tgacgaccgg cgccgatggc
    11521 cgtgtctacc tgagctggat cgaaccggcc ggcggcgaga cgcatcggct gcgtttctcg
    11581 acgtgggacg gcgacggctg gtccgcccca cgtacggtcg cggccggcga cggctggttc
    11641 gtcaattggg ccgacttccc gtcgatggcc gcgctggccg acggaacgtt ggccgcgcat
    11701 tggctcgtgc ggagcgacga gtcgtcgtac gcgtacgacg tgctgctcgc cgtctcgtcg
    11761 gacggcggcg cgagttggag cgagccgacc gccgtgcacg ccgaccgaac gcagaccgag
    11821 cacggtttcg tctcgctgct gccggcgaac ggcgacgagt tccacgtgat ctggctcgac
    11881 ggccgggagc tggcgggcgc cgatcacggc ggcggcgcga tgacgctgcg tgcggcgagg
    11941 ttcgagcgga acgggctgcg gagcgggccg gaaagcctga tcgactcgcg cgtctgcgat
    12001 tgctgtcaga ccgacgcggt cctcgcggcg ggcggcacgc cggtcgtggt ctaccgcgac
    12061 cggacggacg acgaggtgcg ggacatctcg atcgtacgcc gcgaggacga cgcgtggagc
    12121 gagcccgtcg cggtccatca cgacgactgg cggatgccgg cctgcccggt caacgggccc
    12181 gcggtcgacc acgcgtcggc gaggttggcc gcggcttggt tcaccgctcc cgacgggagc
    12241 ggtcgcgtcc aggtcgcgtt ctccgacgac ggcgggcgga cgttcgggcc gcccgtgcga
    12301 ctcggcggcg atcgcgcggt gggtcgtgtc gatctggtga tgctcgacga cgggtcggca
    12361 ctcgtcagct ggctcgagga gtcgcggctg tcgctacagc gcgtcgggcc gaacggaacg
    12421 cgcggtccga tccacgccgt cgcccgcacg agcaacgagc gcgcggcggg ctttccacga
    12481 atgacgcgcg ccggcgagct gctgttcttg acgtggaccg agcccgcgga gccctcccgc
    12541 gtgcgagcgg ccgtggtggc gctcgccgac ctgtgacgac cgggccgttc tttacagcat
    12601 ccggcgcgta aggtacgatc gttgttcggt ctggtgcgca cccgatcgtg ccgcactcca
    12661 agatccgggg gaagcggacc acccaccggc gccccgggaa atctcccggt cccgtcgggc
    12721 cgaacaccca ggctgggagt cctggcatca gcgaggacaa tccgatggca cgaaggtcac
    12781 gtacatccat cctcaagcga cagcgcgaac tgcgcaaggc ggagaaggcg gcgcagaagc
    12841 gtgcgcgccg tcacggcgtc agtctcgatc cgatgaagga gcccgtcccg acggtctcgc
    12901 tggcggacct gctgcgcgcc ggctccgatc ggaccaacca ggaaccagac gacgacggct
    12961 cgtagcgcaa gcctggcgcg gctggagtcg agtaccggcg cctcgatcct gtaccccgca
    13021 cgatggtcgc acgacggcgt gaatcgatcc cgcgagcatc gacagcccgc tgggcaccgc
    13081 gaaccactcc tcccacgacg catcgacgag gtacgcgcgc ggtgcgcaac gtcgctcagc
    13141 gaccgccggt ggcaccgtca cccgagacga acgcgacgac ccgatcgacg atcgcgcggt
    13201 cgcgcaggat gccgcgatgt cccagcccgc cggtcgcgat cagcgatgcc gccggccaag
    13261 cctcggccaa ctcgcggctt tgcgcgaacg gcacgtcgcg atccgcgcgg tcgtgcacca
    13321 gcaatgccgg cggcagggcg cgccggtacg cgatcgccgt cccgtcgagc gcggcgagcc
    13381 gaatcgagaa gcgccgttcg atacggcgca ccatcctccg ctccaccgtc tggggcagac
    13441 cgagcgcggc gcagaacgtc tcgatgaagt agcgcaagcg cgcgggcgcc gcgaggtagg
    13501 cgacgcgccc gacgtcgaga ccgccatcga gcgcgaccgt cgtcgccgcc gctcccagcg
    13561 agtgcgcgat caccgcgacg agcggtccgc tcgccgcgcc gaccgtacgt accgcctcgg
    13621 ccagctccgg cagcgacgat cggcggcccg ccgaccgtcc gtggcccggc ccgtcgtaac
    13681 cgacgacacg aaagccacgc tcgaccagcg gttcgacgaa cgagccgagc tgcgagccgc
    13741 gtccacccca gccgtggacc agcaggacgg tcgggccccg gccgatgctc cagccgtgca
    13801 ggcggcgtcc ggccgcgtgg aactcgtccg gcaccgtgcc ccaggtccag cgagtctcac
    13861 gctccgggac ctgcctgcga ggcggggtga ggaacagccc gagcgcggcc gaggcgatcg
    13921 gggcgggggc cgccgtcgcg gcgagccgta ccaccaggcg ggcggcggtg gggagcccgg
    13981 gaagatacga ccgatcggtc gtgctattac cggggtaggt tcgttcggaa tactgcatcg
    14041 acacctccgg ggacgggatc agcggcgggc gcggtcgatc agcgcgtcga acgcgcgacg
    14101 tgcccgacgc tcggcgcgct cgataccgag cgtgcgatgg aagtggttgt acgccagcac
    14161 gattccatag gcctcgaaga cgagctcgtc gacatcgacg ttcgcgcgga agtgcccctc
    14221 ggcgatggcg aggcgcgcgg cggtcttcag cgtctcgacc cattcccgca aggtggcgac
    14281 gagggcatcc cgcggcggcc cgggccggtc gtcgagctcg tgggcgaagg tcatgaagac
    14341 gcacccgcca gggagatcgg ggccgctgtc ccactcgagc cacttctcga acatcgtgcg
    14401 aaaacggggc tcccctcgtg gccgtttgag cgccggggcc acgacccgct cgacgaaccg
    14461 cgcgcgggcg cgctcgatca gctggagttg caggtcttcc ttggagcgga agtgggcgaa
    14521 caggccgctc ttcgacagtc ctacctcccg tgccaggacg gcgagcgaca gcgactccag
    14581 tccgaccgtg ctcgacagcc ggatcgcccg atccaggatc gccgcccggg tctgctcgcc
    14641 tttgatcgcc acgcgtggaa aatagtacga ccggtcggtt ttgtcaacgg gccgatctcg
    14701 acgaggattc gcccgggtta agatggcccg aaatggccga caacatcgag acccacaagc
    14761 gggcgctcca ggtcaatctc gatcgccgga ccttcgggac gttcgccgag atcggcgcgg
    14821 gccaagaggt cgcccgctgg ttcctgcagg tcggtggtgc ggccgggtcg gtcgccaaga
    14881 cgatctcggc ctacgacatg acgttcagcg acgcgatcta cggccgcggg tcgcgttacg
    14941 tctcgcgcga gcggctgttg tcgatgctcg accacgagta cgcgctgttg ctcgagcgct
    15001 tggccgccga ccgcgggacg accacccagt tcttcgtctt cgccgacacg gtcgaagcac
    15061 gcaactactc cggcaccaac gactgccacg gctggctcgg cgtccggttc caggacgagc
    15121 ccggcgggcc gccgagcgac gtacgcctgc acgtcaacct gcgcgatccg accaacctac
    15181 aacagcaacg agccctggga atcctcggcg tcaatctgat cttcgccgcc ctctaccgtc
    15241 gcgactcggc ggcgacgctc ctcgatgcgc tgttcaccga cctctcgctc gaacggatcg
    15301 agatcgacgt cgtcgagctc gccgggccgg cggtcgctcc gctcgacacc gcgcggttgg
    15361 gccagcggtt ggtacgaggc gggctggccc aggcggtact gcttcgcaac gacgggacgc
    15421 tcgttcaacc ctcgagcctg ctgcgcaagc ggccgctcgt cgtcgagcgc gggctgttcc
    15481 cgacggtggc ggcgtttcac cgccaaatgc tcgacgaggc ctgcgccgaa ttgaagcgga
    15541 gcgagaaacc gcagcgcgat ccgctgccgg tccccgagct gtcggtgctc ccggtcgcgg
    15601 gccgtgaggc gccggacgag gccgagacgc accgccgcct gcacgagctg ttggcgctgg
    15661 gcggtcccgt cctgctgacg cgattcccgc aggtctacca cctcacctcc tacctcagac
    15721 gttacacgcg cgagccgctg cgtttcgtct tcggcgcgtc gacactggtc caggtccttc
    15781 acgacgcgca ctacgccgac ttgatgggcg gcgtgctgga ggcgctcgga cgcctgctgg
    15841 cggacaacgt caagatctac ctctacccga tgcaggccga cgttttccgc gagatcgtcg
    15901 agcggcacgg cggtgcgtcg cgggtggcgc cgcccgccag ccagtgggta accgccgaga
    15961 cgatcgagtt cgagccgccg atcggtcatc tcttccgcta cctgcgcgag atggggtggg
    16021 tcgtcggcct cgacccgccg cgggccggtt gagcggcggg gctacttgcc gatgaccgtc
    16081 gtaccggcgg gccagtccgc gcggaaggtc tgctcgggca ggctcggctc gacggtgagg
    16141 tgacgatagc tcacggatac ctgcaacccg ccgttgctct gccggatcag ctgccgcgcg
    16201 ggcagccagg tcgtctcgtc gatccacacc atcaccaccg gcacggacgc ggcggtctcg
    16261 gtcgacctgg gcgtcagctc caacaaaaac gtgttgcggc cgtcgacctc gtcgcgcttg
    16321 acgagtgcga cgtcgtagtc ctgcttgagc ttcgatccgc gtggtgcgaa accgagcatg
    16381 gcgtactgca cgagcaggtc cggctggaac agcgtctcgt atacttccgc cacctgctcg
    16441 agcggcttgt acagctggac gtacgccgga gagagcagca gcgtgcgcgg gttgctcccg
    16501 ccgacctcgg cccgcatccg cccggcgaga tcgatccaca ccttcccgtc gccgtcgtgg
    16561 tcgatcgccg aaccgaggaa ctcgttccac gcgatctccg ccgtgaccca gcgggcttcc
    16621 ttggtctggc ggtcgacctg cttcaacacg ctcttgagcg tccactcctc cgccgcgacg
    16681 ggcaggccgg cgagcagcac gaccgacaac caagaccaac cggcctttct cgcgaacacc
    16741 ggggttcctc ggggcttgcg ttaggatagc ggcacgcccg cgaccaccat actacgaaga
    16801 gcgagccagc gggccagcgg gggaaccgag atgggcaacc gatcgctgcg ctgccggctg
    16861 gtcgccgcgg gtctggtgtc ggcatcgctc gtcggcggag cggcccgggc gggatgggcg
    16921 agctgggacg acgaggatcg acggagatgg ctggaacacc acgcgaaggt cgagaagcgg
    16981 ctgtgggtcc cgatgcggga cggcgtccgg ctgtcgaccg acctctatct gccggccgac
    17041 gccgacgggc cgttcccggt gatcttctgg cggacgccct acaacttcag cgagctcgac
    17101 ggcatgttgc tgtcgttcgc ccacgaatcg gtggcccggg gttacgcgtt cgtgctgcag
    17161 aacgagcggg gcaagttctt ctcggagggc gaatgggtgc tgctcggcct gccacgcacc
    17221 gacggctacg acgcgctcag ttggatcgcc gaacaaccgt ggtcgaatgg caaggtcggc
    17281 acgctcggct gctcgtcgcc ggccgagtgg cagctggcgc tcgccgcgat ggaccacccg
    17341 gcacacgccg cgatggtgcc gatggctccc ggcgccggca tcggccgggt cggcgagttc
    17401 tgggagcagg gcaactggta tcgcggtggg gccgagcaga tgttctacct gccctggctc
    17461 tacgaggtgc aggacacgca gcgcccgcgg ctacccgacg gtctgtcgcg cgaggacctg
    17521 gtgcggctgt cgagttatta cgacctggca ccggagatgc ccgagatcga ttggaagaag
    17581 aagatccgca tcctgccggt cgccgacgtg atggagcggg tcgacgggcc gcgcggcatc
    17641 tataaggagt acttcgcgcg caggcccgac gacccggcat ggttcgacag cggtttgtgg
    17701 catgacgacg aggacttcgc cgtgccgtcg ctgtggctgt tctcctggta cgacatctcg
    17761 accacaccca acctggcgct cgtcgaccac atcgtccgca acgccggcga cgccgaggtg
    17821 cgggacagtc agttcgtgat catggcaccg gtcgagcact gcgcgttctt ccgatccgcc
    17881 tcgccgtaca cgatcggcga gcgcgagctg ggcgacatcg acctcgacta cgaggaacgg
    17941 atctacgcct ggttcgaccg ctggctcaag ggcgaggaca acggcgtcac ccgctcgatg
    18001 ccgaaggtgg agtactggac gtttggggtg aacgagtggc ggaaggccga gcgctggccg
    18061 cccgcgggcg ccgagacgat tccgttctat ctggcgagcg acggcgacgc gaacagcctg
    18121 ttcggcgatg ggcgtctcga gcgcgaaccg ccggcggaga accggcccga ccgcttcacc
    18181 tacgacccga ccgtgccggt cccgtcgctc gggggcggcg tctgctgcat cggcgggacg
    18241 atcgagcccg gcgcgttcga ccagcgcgcg atcgaggcgc gggccgacgt gttggtctac
    18301 acgagccgac cgctcgagcg ggacctcgac gcgaccgggc ccgtgcgggt cacgctgttc
    18361 gtctcgtcgg acgcgcccga caccgacttc acggtcaagc tcgtcgacgt cgctcccgac
    18421 ggcacggcct acaacctcga cgagacgatc caacgcgtgc gctaccgcgc gggctacgat
    18481 cgcgaggtgt tgatgaagcc gggcgaggtc tacgagctgg cggtctcacc gatgtcgaca
    18541 agcaacgtct tcctcgccgg tcaccggatc cggatcgagg tgtcgtcgag caacttcccg
    18601 cgcttcgcgc gcaacctgaa caccggtggg ccgaaccaca acgagtccga gcccgccgtc
    18661 gcgcgcaacg tggtccatca ctcgaagcgc tatccctcgc gcatcgagct gacggtgctg
    18721 ccgtgatcgc cggcgcgtcg cgaaccggcc gcctcactcg tgcgagtctc gggcgacgag
    18781 cgtcccgcgc gccggcgccc acggcccgac gagccggctg gcgtagccgg cacggcccag
    18841 ttcggccatc tcggccgtca ggctgtccgc ccgcggctcg atcagggcga agcggccggc
    18901 gaagtgggcc acgatcgcgt acccctcccc ggccgaccgc ggcgacggca ggtcggcgta
    18961 gatctcgccg ccatattccg cgcgcgcgta ggtgaagagc tgagcccaat cggcgaggcc
    19021 gtagggcgtg accgttcgct tgaagtacag cttgggcagt ccgagcgcgc cctcgcggtc
    19081 caggcgtccg agcgggtcga cgcggtccat gcgcacgcag aggatcggcc ggtcggtcgg
    19141 caaccgcggt gcgaaggcgc ggatctcgtc gaccagttcg tcgtggctcg cgcgcagccg
    19201 tcgcatgtcg gccagatcgc cgcgcaacag cagcacaccc gcgccgaaca gggccagcgc
    19261 tccggccgcc gccgcgacga gcgccagccg gcggcggcgc accggcgacc agcggcacag
    19321 cgcgtcgacc gtcccggtca tcaccagcac gaagccgacc aacggcgccg tcgtgtagcg
    19381 cgtcggattg aacgggatga acagcaccgg cgccatcgcg cacaggaaga aaacgaagcc
    19441 gaggccgacc aagtggcttc gctgccgcca gccgatcatc gcgcaaccgg cgatcgcgcc
    19501 gagcgcggcc agttcggcga tgccgaaggc ggcgcgggtc gactcgaccg tcgtgagcat
    19561 catgaagccg gcccagtagt gcggcagctt ggccagcacc cacgagcccg cctcgtagta
    19621 accgccgcgc ccgggaaaga gccaggggta ggccacgacg tacgccgctg ccggaatcgc
    19681 gagcagcgcc gccgtgcgca gcgcgcgccg cggccggcgc cgcgcgatga acagctcgaa
    19741 gccgacgacg aggccgggga ggatcaccca gctttctttg cagaagacgg agcacagagc
    19801 aatgccgacg acggcggcga gccggccgcg cgaccagctc ccggtgggac cgggccacgc
    19861 ggccgcgatg gcgagccacg ccaacagcac ggtcagg
//
LOCUS       DASPGN010000395         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1594191, whole genome shotgun sequence.
ACCESSION   DASPGN010000395 DASPGN010000000
VERSION     DASPGN010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594191"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="VD788_11475"
     CDS             complement(<1..1097)
                     /locus_tag="VD788_11475"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXV76924.1"
                     /translation="MSEAGRRPNHLAGETSPYLLQHLYNPVDWYPWGADAQHKARRED
                     KPIFLSIGYAACHWCHVMERESFENAAIAERLNRDFVSIKVDREERPDIDEIYMNAVQ
                     LMTGAGGWPLSAFLTPDLEPFYAGTYFPPDDRYGRPGFGTLLAEIRRAWTERRDQIVA
                     SAGKITDSLRAIADGVSDGEPHGTIDENLQRIAVADWTRRFDPRWGGFGSAPKFPPDG
                     ALAVLLREHGRTGEKVPLSVATRTLDGMAGGGLYDHVGGGFSRYSVDERWLVPHFEKM
                     LYNQALLVPLYVDAYKITGEARYRRVVEQTLDFVRRELTDDDGGFYSSLDADSETEEG
                     KFYVWTPREIGSLLDAEEAKLFCSLYGIDEAG"
     gene            complement(1094..>2520)
                     /locus_tag="VD788_11480"
     CDS             complement(1094..>2520)
                     /locus_tag="VD788_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76925.1"
                     /translation="GQNAACGTARRFQIEESWQTDKSLRLLSLTLGQLAGLDPPKVVF
                     YFADMMRQQAGAHFFSYIGSCSLTADDGVGRQSGFGAEFAIDSVIEQAASYGTRVYAV
                     RGEGLVASSTSSRRMAFDNVAATNQQRYAHAGDSLAGLSIETGGRSFIHGETPAKIVQ
                     AVEQDMGCLYLVSFDPAGLPLDVPLSVSVEPRSRGVKIHTRGRIVIQSDRTRQISELM
                     AAFASPRAGSADAPIFGAVIPTGFADGRYDALVQLRVPGSPLARAEWHLGASLLTRGE
                     LNDNVSAQIALDRPGVPAVFETQLGFRPGPYELILVAHDAVADQIGADRLEGGWPLRD
                     EGPVVGPIAIMQPASGAFVRDGEVRTHGALGLTEREPLRAAVPTAFVGLVCRGPEKGR
                     LLVHRSLSGMTEHPFPEMEVDLEVERCAQIRDLIPEGTMTPGRFAYRVRLLRDERTVA
                     EGERSFDVVGVQETAPSATEGSDE"
BASE COUNT          391 a          880 c          858 g          391 t
ORIGIN      
        1 cccgcctcgt cgataccgta cagcgaacag aacagcttcg cttcctcggc gtcgagcaac
       61 gagccgatct cccgcggcgt ccagacgtag aacttacctt cctcggtctc gctgtcggcg
      121 tcgagcgaag aatagaaccc accgtcgtcg tcggtcagct cgcggcgtac gaagtccaac
      181 gtctgttcga cgacgcgccg gtaacgcgcc tcgccggtga tcttgtaggc gtcgacgtac
      241 agcggcacga gcagcgcctg gttgtacagc atcttttcga aatgcggcac gagccaccgc
      301 tcgtcgacgc tgtaacgcga gaacccgccg ccgacgtggt cgtagaggcc gcccccggcc
      361 atcccgtcga gcgtgcgcgt cgcgacggac agcggcacct tctcgcccgt ccgcccgtgc
      421 tcgcgcagca agacggcgag ggcgccgtcc ggcgggaact tgggcgccga gccgaagccg
      481 ccccagcgag gatcgaaccg gcgtgtccag tcggcgacgg cgattcgttg caggttctcg
      541 tcgatcgtgc cgtgaggctc gccgtcactc acgccgtcgg cgatcgcacg caggctgtcg
      601 gtgatcttgc cggccgaggc gacgatctgg tcgcgccgct cggtccaagc gcgccggatc
      661 tcggccagca gcgtgccgaa cccgggccgt ccgtagcggt cgtccggcgg gaagtaggtc
      721 ccggcgtaga acggttcgag atcgggggtg agaaaggcgc tgagcggcca gccgcccgca
      781 ccggtcatca gctggacggc gttcatgtag atctcgtcga tgtccgggcg ctcctcgcga
      841 tcgaccttga tcgagacgaa gtcgcgattg agccgttcgg cgatcgccgc gttctcgaac
      901 gattcacgtt ccatcacgtg acaccagtga caggcggcgt aaccgatcga gaggaagatc
      961 ggtttgtcct cgcgacgggc cttgtgctga gcgtccgcac cccacgggta ccagtcgacc
     1021 gggttgtaca ggtgttgcag caggtagggg ctggtctcgc cggcgaggtg attcggccgg
     1081 cgtcctgctt cgctcattcg tccgatccct cggtggcgga cggcgccgtc tcctgcacgc
     1141 ccaccacgtc gaagctccgc tcgccctcgg ccaccgtccg ctcgtcccgc agcaggcgga
     1201 cccgataggc gaaccggccc ggggtcatcg tcccctcggg aatcaggtca cggatctgcg
     1261 cgcaacgctc gacctcgagg tcgacctcca tctcggggaa cgggtgctcg gtcattccgc
     1321 tcagcgagcg gtggaccagc agccgtccct tctcgggtcc gcggcagacc aagccgacga
     1381 acgccgtcgg caccgccgca cgcagcggct cgcgctcggt caggccgagc gcgccgtggg
     1441 ttcgcacctc tccgtcgcgg acgaacgcgc cgctcgccgg ttgcatgatc gcgatcggac
     1501 ccaccaccgg cccttcgtcg cgcagcggcc agccgccctc gagccgatcc gcaccgatct
     1561 ggtcggcgac ggcgtcgtgc gcgacgagga tcagttcgta cggacccggc cggaacccga
     1621 gctgcgtctc gaagacggcc ggcaccccgg gccgatcgag cgcgatctgc gcggacacgt
     1681 tgtcgttcag ctcgccacga gtgagcagcg aggcgccgag gtgccattcg gcacgcgcga
     1741 gcggcgagcc gggaacgcgg agctgaacca gcgcgtcgta gcggccgtcg gcgaagccgg
     1801 tcgggatgac cgcgccgaag atcggcgcgt cggccgaccc ggccctcggt gaggcgaacg
     1861 cggccatcag ctcggagatc tggcgcgtcc ggtcgctctg gatcacgatg cggccgcggg
     1921 tgtggatctt gaccccgcgc gaccggggct cgacgctgac gctcaacggg acgtcgagcg
     1981 gcagaccggc gggatcgaag ctgacgaggt agaggcagcc catgtcctgc tcgaccgcct
     2041 ggacgatctt ggccggcgtc tcgccgtgga tgaacgatcg accgccggtc tcgatcgaca
     2101 ggccggcgag actgtcgccg gcgtgggcgt accgctgttg attcgtcgcc gccacgttgt
     2161 cgaaggccat ccggcgcgag ctggtcgagg aggcgaccag accctcgccg cggacggcgt
     2221 agacccgcgt cccgtacgat gcggcctgtt cgatcacgct gtcgatcgcg aactcggcgc
     2281 cgaagcccga ctggcgtccg acaccgtcgt cggccgtcag cgagcaactg ccgatgtagc
     2341 tgaagaagtg ggcgccggcc tgctggcgca tcatgtcggc gaagtagaac acgaccttcg
     2401 gcgggtcgag accggccagc tggcccagcg tcagcgacaa cagccgcagc gacttgtccg
     2461 tttgccacga ttcctcgatc tggaaccgcc tggccgtccc gcacgcggcg ttctgcccca
//
LOCUS       DASPGN010000396         4810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1125752, whole genome shotgun sequence.
ACCESSION   DASPGN010000396 DASPGN010000000
VERSION     DASPGN010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125752"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..1376
                     /locus_tag="VD788_11485"
     CDS             90..1376
                     /locus_tag="VD788_11485"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HXV76926.1"
                     /translation="MAYKPYNSFAIAQAQFDGAAAILGLDEPTRELLRQPMREYAFAI
                     PVRLDDGKTKVFRGFRVQHNDARGPCKGGIRFHPQETQDTVRALAMWMTWKCAVVDLP
                     LGGAKGGVICDPHNLSDREQEQVCRGWVRQMARNVGPQTDVPAPDVMTSAQHMLWMLD
                     EFEGIRGAKLPGFITGKPVSVGGSLGRTEATGYGVLFTVREALKAMNVRASDTTASVQ
                     GFGNVAQHAIRLYEQMGGTTVCVSCWDQQDKTSYAYQRASGVKLDELLSITDRFGGID
                     KNKAKQLDYRVLPGEEWLKQDVDILIPAAMENQITEENVQQIAPRVKIIAEGANGPTT
                     TEADTVLRQREVEVIPDFLANAGGVTCSYFEQVQSNMNYYWAKDEVLGKLDVKMTSAY
                     HAVSEVARKKKLYMRDAAYIIAVDRVARACRDRGWV"
     gene            1416..3155
                     /locus_tag="VD788_11490"
     CDS             1416..3155
                     /locus_tag="VD788_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXV76927.1"
                     /translation="MGRGFFDRREVPRFDRAFFRSRDAVMRIGDGELGGKARGLVLVR
                     DLLAAEREAVGGGEIEIYLPTMAVLATDVFDAFIARNGLVELACSERSDEQIAAAFLR
                     ADLPVEVAGDLRALVEEVREPLAIRSSSLLEDALFRPFAGVYATKMVPNNQPDPDIRF
                     RRLAEAIKFVFASTWFRGAREYHRAAGRAGDDEKMAVVIQEVVGSGHVDRFYPNVSGV
                     GRSYNFFPSSGAAPEDGVVNLALGLGKTIVDGGVSWIYSPARPHAPAPFASVGDRMRQ
                     TQLEFWAVNVGPPPAYHPTEETEFLVKLDLAAADYDDTLSPIASTYDVTRDRLVAGTA
                     IDGPRVVDFAPMLTDPGARYNDVIRTLLALGRDLLGQAVELEFAIKLARRASAEPARF
                     GVLQLRPMLVAEAEIDITDDELSSPRLLLASKRVMGNGSIDSIRDVVYVRPDRFEARW
                     SRQVAAQLERINRTLIDQSRPYLLIGFGRWGSSDPWLGVPVDWSQVSGARVIVESSLA
                     GMDVEPSQGAHFFHNLISFEVCYLCVRQVDWEWLERFHPVGETELLRHVRLDQPLTVK
                     VDGRRGRGGVWHR"
     gene            3175..>4810
                     /locus_tag="VD788_11495"
     CDS             3175..>4810
                     /locus_tag="VD788_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXV76928.1"
                     /translation="MSNDTPIQQLIESLQERAKELNCLYRVDEILVDPDRSIAQVGAE
                     IPDALPPGWKYPDACAVHLRLNDEEFQTANFRPTPWFLTAPVLLEAREIGLLSVHYTE
                     PRPPADEGPFLKEERKLINTLAERLGFFVTRRNLHRAHESLQSARDLSQRKERAEWYV
                     ILEFLRRTDPELLRRITRRMINHLELSGVAGAVGLLRDAALLEEAVPSLAGENVPLRK
                     SRFRDFTSLSEQTLQIAADNLGVEEMVASIRNWIDEERSSFLIEALEDQSRPLSAVVE
                     SIERHRSHVGQTDHLPVPFRRTLNAALLRRLFSDETAYINVGKDVVEATDVYDLVNRI
                     IYPLHGHGKLGGKSAGLFLAEHILRRAGESCEPLRGIKAPPTWYVSSNALLEFLNYNN
                     LRELYFRKYMHIEQVRQEYPHVVQLFKNSHFPPEISRGLSLALDDLGDRPLIVRSSSL
                     LEDRIGSSFAGKYKSLFLPNRGTREERLEALRDAIAEVYASAFAPDPIEYRADRGLTD
                     VHEEMGIMIQSVVGSRVGRYFLPAFSGVALSRNEFRW"
BASE COUNT          807 a         1606 c         1608 g          789 t
ORIGIN      
        1 cgcgccgaga gacgttaccc tcggcgtcgc cgagtgcgcc gaccgctctc ggccgacgag
       61 tgacgcgtcc cgagcgatca ggagtatcga tggcctacaa gccctacaat tcgttcgcca
      121 tcgctcaagc ccagttcgac ggggcggcgg cgatcctcgg actcgacgaa ccgacccgcg
      181 agctgttgcg ccaaccgatg cgcgagtatg cgttcgcgat tccggtgcgt ctcgacgacg
      241 gcaagacgaa ggtcttccgc ggattccgcg tgcagcacaa cgacgcgcgc ggcccgtgta
      301 agggcggcat tcgtttccat ccgcaggaga cgcaggatac ggtccgagcg ctggcgatgt
      361 ggatgacctg gaagtgcgcc gtggtcgact tgccgctcgg cggagccaag ggcggcgtga
      421 tctgcgatcc gcacaatctg agcgaccgcg aacaggagca ggtctgccgc gggtgggtcc
      481 gtcagatggc gcgcaacgtc gggccgcaga ccgacgtgcc cgcgcccgac gtgatgacca
      541 gcgcccagca catgctgtgg atgctcgacg agttcgaggg catccgcggc gccaagctgc
      601 ccggcttcat caccggcaaa ccggtcagcg tcggcggctc gctcggtcgc accgaggcga
      661 ccgggtacgg cgtgctgttc accgtgcgcg aggcgctcaa ggcgatgaat gtccgggcgt
      721 cggacacgac ggcgagcgtc cagggattcg gtaacgtcgc ccagcatgcc attcgactct
      781 acgagcagat gggcggaacg accgtctgcg tctcctgctg ggaccagcag gacaagacgt
      841 cctacgccta ccagcgggcg agcggcgtga agctcgacga gctgctgtcg atcaccgacc
      901 gcttcggcgg gatcgacaag aacaaggcga agcagctgga ctaccgggtg ctgccgggcg
      961 aggagtggtt gaagcaggac gtcgacatcc tgattccggc ggcgatggag aatcagatca
     1021 ccgaagagaa cgtccagcag atcgccccgc gggtcaagat catcgccgaa ggggccaacg
     1081 gccccaccac gaccgaagcc gatacggtgc tgcggcagcg cgaggtcgag gtcattcccg
     1141 acttcctggc caacgccggc ggtgtgacct gcagctactt cgagcaggtc cagagcaaca
     1201 tgaactacta ctgggccaag gacgaggttc tcggcaagct cgacgtgaag atgacctcgg
     1261 cctaccatgc ggtcagcgag gtggcgcgaa agaagaaact gtacatgcgc gacgcggcct
     1321 acatcatcgc ggtcgaccgc gtcgcgcgcg cctgccgtga ccgggggtgg gtctgagcgt
     1381 tcccctcgcc cgcgggccgt tcgtcacgag gagcgatggg ccgaggattc ttcgatcgac
     1441 gcgaagttcc gcgttttgac cgcgcgttct ttcgttcgcg cgacgccgtc atgcgcatcg
     1501 gcgacggcga gctgggcggc aaggcgcgcg ggctcgtgct cgtccgcgac ctgctcgcgg
     1561 cggagcgcga ggctgtcggc ggtggtgaga tcgagatcta cctgccgacg atggcggtgt
     1621 tggccaccga cgtcttcgac gctttcatcg cgcggaacgg tctggtcgag ctggcgtgct
     1681 ccgagcggtc cgacgaacag atcgcggcgg cgttcttgcg ggcggatctt ccggtcgagg
     1741 tcgcgggcga cctgcgggcg ttggtcgagg aggtgcgtga gccactggcg attcgctcgt
     1801 cgagtctgct cgaagacgcg ctgttccgtc cgttcgccgg cgtctacgcg acgaaaatgg
     1861 tgccgaacaa tcagccggat ccggacatcc gcttccggcg gctcgccgag gcgatcaagt
     1921 tcgtcttcgc ctcgacctgg ttccgcggag cgcgcgagta ccaccgcgcc gccggacgcg
     1981 ccggcgacga cgagaagatg gcggtagtga tccaggaggt cgtcgggagc ggtcatgtcg
     2041 atcggttcta tcccaacgtg tcgggcgtcg gtcgctccta caacttcttc ccgtcgtccg
     2101 gcgcggcgcc ggaggacggc gtggtcaatc tggcgctcgg cctcggcaaa acgatcgtcg
     2161 acggcggcgt gagctggatc tactcgcccg cgcggccgca cgcgccggca ccgtttgctt
     2221 cggtcggcga tcgtatgcgt cagacgcagc tcgagttctg ggcggtcaac gtcggcccgc
     2281 cgccggccta ccacccgacc gaggaaacgg agtttctcgt caagctcgac ctggccgccg
     2341 cagactacga cgacacgttg tcgccgatcg cgtcgaccta cgacgtcacg cgtgaccggc
     2401 tcgtcgcggg caccgcgatc gacgggccgc gcgtcgtcga tttcgccccg atgctgaccg
     2461 atcccggcgc gcggtacaac gacgtgatcc gcacgctgct cgcgctcggg cgcgacctgc
     2521 tggggcaggc ggtcgagctc gagttcgcga tcaagctcgc gcgtcgcgcg tcggcggaac
     2581 ccgcccgctt cggcgtactg cagctccgac cgatgctggt cgcggaagcc gagatcgaca
     2641 tcaccgacga cgagctgtcg tcgcccagac tgctgctggc gtcgaagcgg gtgatgggca
     2701 acggctcgat cgattcgatc cgcgacgtgg tctacgtccg tcccgatcgg ttcgaggcgc
     2761 gatggagccg acaggtcgcc gcccagctcg agcgcatcaa ccgcacgctg atcgaccagt
     2821 cccgccccta cctgctgatc ggcttcggcc gctggggcag ctccgacccg tggctcggtg
     2881 tcccggtcga ctggagccag gtctccggcg cacgcgtgat cgtcgaatcg agcctggccg
     2941 gaatggacgt cgagccgagc caaggcgccc acttctttca caacttgatc agcttcgagg
     3001 tgtgctattt gtgtgtgcgg caggtcgatt gggagtggct cgaacggttc caccccgtcg
     3061 gcgaaaccga gctgctgcgc cacgtccggc tcgaccagcc gctgaccgtc aaggtcgacg
     3121 gtcgccgcgg tcggggaggc gtctggcacc gctagcgcgg gaagggtccg tcgagtgtcg
     3181 aacgacacgc cgattcagca gttgatcgaa tcgctgcagg agcgcgccaa agagctcaac
     3241 tgtctctacc gcgtggacga gatcctggtc gaccccgatc gatcgatcgc ccaggtcggc
     3301 gccgagatcc ccgacgcgct tccccccggt tggaagtacc ccgatgcgtg cgcggtccat
     3361 ctgcggttga acgacgagga gttccagacg gcgaacttca ggcccacgcc gtggttcctc
     3421 accgcgccgg tgctgctcga ggcgcgcgag atcggtttgc tgtcggtcca ctacaccgag
     3481 ccgagacccc ccgccgacga aggcccgttc ctcaaggagg agcgcaagct gatcaacacc
     3541 ctcgccgagc gcctcggctt cttcgtcacc cgtcgcaacc tgcaccgcgc gcacgagagc
     3601 ctgcagagcg cgcgagacct gtcgcaacgg aaggagcgcg cggagtggta cgtgatcctc
     3661 gagttcctcc gccgcaccga ccccgagctg ctgcggcgca tcacgcggcg gatgatcaac
     3721 cacctcgagc tgtcgggcgt cgccggtgcc gtcggactgc tgcgcgatgc cgcgttgctc
     3781 gaggaggcgg ttccttcgct cgccggtgag aacgtacccc tgcgcaaatc ccgcttccgc
     3841 gatttcacct cgctcagcga acagacgctg cagatcgccg cggacaatct cggcgtcgag
     3901 gagatggtcg cctcgatccg caactggatc gacgaggaac gctcgagctt cttgatcgag
     3961 gcgctcgagg atcagagccg gccgctgtcg gccgtggtcg aatcgatcga gcggcaccgc
     4021 agccacgtcg gccagaccga tcacctgccg gtcccgttcc ggcggacgct gaatgccgcg
     4081 ttgttacgcc ggctgttcag cgacgaaacc gcctacatca acgtcggcaa ggacgtggtc
     4141 gaagcgaccg acgtctacga tctggtcaac cgcatcatct acccgctcca cggccacggc
     4201 aaactcggcg ggaagagcgc gggcctgttc ctcgccgagc acatcctgcg ccgggccggc
     4261 gagtcatgcg agccattgcg cggaatcaag gcgccgccga cgtggtacgt cagctcgaac
     4321 gccctgctcg agttcctcaa ttacaacaac ctccgggagc tctacttccg caagtacatg
     4381 cacatcgagc aggtccggca ggagtacccc cacgtcgtgc agctgttcaa gaactcccac
     4441 ttcccgccgg agatctcccg cgggctgtcg ctcgccctgg acgacctcgg cgaccggccg
     4501 ttgatcgtgc gtagctcgag cctgctcgag gatcggatcg gctcgagctt cgccggcaag
     4561 tacaagagcc tgtttctgcc gaaccgtggc acgcgcgaag aacgcctcga ggcgctacgc
     4621 gacgcgatcg ccgaggtcta cgcgtcggcg ttcgcaccgg acccgatcga gtaccgtgcg
     4681 gatcgcggac tgaccgacgt ccacgaggaa atggggatca tgatccagtc ggtcgtcgga
     4741 agccgcgtcg gccgctactt ccttccggcg ttctccggcg tggcgttgag ccgcaacgag
     4801 ttccgctggt
//
LOCUS       DASPGN010000397         5871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_307662, whole genome shotgun sequence.
ACCESSION   DASPGN010000397 DASPGN010000000
VERSION     DASPGN010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307662"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VD788_11500"
     CDS             <1..368
                     /locus_tag="VD788_11500"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXV76929.1"
                     /translation="AAIEQAAIVARGDVIEPEDLPSQVRESRQSTPTGAAADRDPSKR
                     PFRDAKRDVVEVFERGYLGALLERHAGNVTAASQQAGMLRSALQRLLRKYGLKSAEFR
                     KGRRPGVRAVAGESPDRSR"
     gene            365..1234
                     /locus_tag="VD788_11505"
     CDS             365..1234
                     /locus_tag="VD788_11505"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HXV76930.1"
                     /translation="MTARTEYWRLLIDTPADGAWNMAVDEAILDGYTATTGAIRPPTL
                     RLYGWRPAALSLGKSQPAGESHDAPALRAAGVDLVRRPTGGWAVLHDHERTYALTGPL
                     RAPSFASGVLTTYRRVADALILALGGLGVEVGASGEAPARTTTRASAACFTVTSAYEI
                     TAGGRKLVGSAQLRRRGGFLQHGSIPIRIDAARLAVVLGREIDAERFTDLGTALGRIP
                     DPDEVDRALCAGFSQAFDVKLRRGRLTADERLRATRLRAWKHLSAAWVVGGKAGRREA
                     AWGPTARASSAAS"
     gene            complement(1215..3830)
                     /locus_tag="VD788_11510"
     CDS             complement(1215..3830)
                     /locus_tag="VD788_11510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HXV76931.1"
                     /translation="MHPSQFPPAPGNPPPRSPDAHPPFYLRRGPYLVMAGCVLGAALA
                     GLVFGRFVRFDLPDVRGLEDYNPPVMTRVMARDGSMIASFAEERRMLIDLPDIPQVFI
                     QALVATEDSSFYRHPGIDVKGIVRALWSDLRHMRMKEGASTLTQQLARNLFLHPDKTI
                     RRKLQEVVLALEIERQYTKQEILRFYCNQIYTGHGRYGIEAAARYYFGKPAREMSLVE
                     SATLAGLIQRPEALSPLRSPQRSIERRNYVLSRMADEGLLSEAAAAEAQKQPLEVAQL
                     RRTDDPAPYFVEDVRRWLQAEYGSSSLYKEGLVVSTTLDPDLQQIANRAMDVGLRQLD
                     RRQGWRGVTQRVPDGEDPLIWQSADWAAGIRPGEVHDGIVIDVDSERARVRVASYEGN
                     LTRERIAWTAIARPDKLLDVGDVVRVRVESTDEQGRARLTLEQEPLAEAAIVVLEPTS
                     GAILAMVGGFDFERSEFNRATQARRQTGSAFKPFVYAAALSEGWTLAHTLVDEPTVFL
                     DRRNPEPYQPENYTNEYYETVTLRSALEKSANIATVKLLTEVGYSPVIDTARRLGISG
                     DLRPYPSLALGAFEVTLLELTAAYGAFANQGVLVEPHLVRDVHNREGALVAQIEPEVR
                     DAVRPEIAYLMNRVLSGVISDGTGRAASRLGSNVAGKTGTTDHNTDAWFVGYSPNLAV
                     GVWVGFDEPRSLGSRETGAIAALPIWQEFMEQALPLRPAGDFPQPANISVQSVDRRTG
                     LKANLRAGCNPVISEVFVSGTEPTTYCTDEHHIKLGFPYPFHRHALTERGALAIPSDE
                     LSRLLDTEADVFLVDGGTQLEAHTPQGASYSLALEVLPPEPPPPWPGRLDERFDRSTW
                     VGKDGRSARVSWLRN"
     gene            3967..>5871
                     /gene="uvrB"
                     /locus_tag="VD788_11515"
     CDS             3967..>5871
                     /gene="uvrB"
                     /locus_tag="VD788_11515"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXV76932.1"
                     /translation="MQPFELVSGFEPTGDQPAAIDALARGLTDGLPDQVLLGVTGSGK
                     TFTIANLIARAQRPTLVLAHNKTLAAQLYQEFRRLFPRNAVEYFVSYYDYYQPEAYVP
                     QSDTYIEKEATINEEIDRMRHSATRALFERRDVLIVASVSCIYGLGSPEAYHGMMLLL
                     ERGMTIDRDAALRKLVEIQYERTGADLRRGAFRVRGDTVEIWPSYADDAYRIEFWGDQ
                     IEAIARIDPVRGVRLESLDRHPVYPNSHYVTPRETLIRAMESIRTELGERVAALERSG
                     RLLEAQRLKQRTSFDLEMLSELGYCHGIENYSRHLTGRQPGEPPPTLMDYLHDDCLLI
                     VDESHATLPQVRGMYHGDRSRKLTLVEHGFRLPSAVDNRPLTFDEFEQRRGQAIYVSA
                     TPGELELARAGGEYVQQVVRPTGLLDPATDVRPVNGQVDDLLAEIRRRTDRGERTLVT
                     TLTKRMAEDLTDYYRELGVRVEYLHSEVVTLERVRILRDLRRGEFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGYLRSEPSLIQTIGRAARHVNGSAILYADRVTGSMRRALEET
                     SRRRGIQQAYNERHGITPETVRRAMDELIGTPIAADYSTVPLDEPQDEPVFENLDKLD
                     EEIGRLEKKMH"
BASE COUNT          904 a         1999 c         2055 g          913 t
ORIGIN      
        1 gagcggcgat cgagcaggcg gcgatcgtgg cccgcggcga cgtgatcgaa ccggaggatc
       61 taccgagcca ggtgcgcgag tcgcgccagt cgacgccgac gggcgcagcc gcggatcggg
      121 atccgtcgaa gcgacccttc cgtgacgcca agcgcgacgt ggtcgaggtt ttcgagcgcg
      181 gctacctggg cgcgctgttg gagcgacatg ccggcaatgt gacggccgcc tcgcagcagg
      241 ccggcatgtt gcgttcggcg ctgcaacgac tgctccgcaa gtacggtctc aaatccgccg
      301 agttccgtaa gggtcgccgg cccggcgtgc gtgccgtcgc cggcgagtcg cccgaccgct
      361 cgcgttgacc gcgcgaacgg agtactggag actgttgatc gatacgcccg ccgacggtgc
      421 gtggaacatg gcggtcgacg aggcgatcct cgacggctac acggcgacga cgggggcgat
      481 ccgacccccg accctgcgct tgtacggctg gcgaccggcc gcgctgtcgc tgggcaagag
      541 ccagcccgcc ggcgagagcc acgacgcgcc ggcgctccgc gccgcggggg tcgatctcgt
      601 gcgacgtccg accggggggt gggccgtgtt gcacgatcac gagcggacct acgcgctgac
      661 cggcccgctg cgagcaccgt cgttcgcctc gggcgtgctg acgacctacc ggcgcgtggc
      721 cgacgcgctg atcctggcgc tcggcggtct gggcgtcgag gtcggcgcga gcggggaggc
      781 gccggcgagg accacgaccc gcgcgagcgc tgcgtgtttc accgtgacct cggcctacga
      841 gatcaccgcc ggcggtcgca agctcgtcgg ttcggcacag ctccgcagac ggggtggctt
      901 cctgcagcac ggctcgatcc cgatccgcat cgacgcggct cgcctcgcgg tcgtgctcgg
      961 ccgggagatc gacgccgagc gcttcaccga cctcggcacc gcgctcggtc ggatccccga
     1021 cccggacgag gtcgatcgcg cgttgtgcgc ggggttctcg caggcgttcg acgtcaaact
     1081 gcgccgaggg cggctgacgg cggacgagcg gctgcgggcg acgcggctac gcgcgtggaa
     1141 gcacctgtcc gcggcctggg tcgtcggcgg gaaggcgggc cgacgcgagg cagcctgggg
     1201 accgacggct cgagctagtt ccgcagccag ctgacacggg cgctgcgtcc gtccttgccg
     1261 acccacgtgc tgcgatcgaa gcgctcgtcc aatcgcccgg gccacggcgg cggcggttcg
     1321 gggggcagca cttcgagggc cagcgaatag gaagccccct gcggggtatg ggcttcgagc
     1381 tgcgtaccac cgtcgacgag gaagacgtcg gcttccgtgt cgagcagccg cgacagctcg
     1441 tccgacggga tggccagcgc gccacgctcg gtcaacgcgt ggcgatggaa cgggtaaggg
     1501 aagccgagct tgatgtgatg ctcgtcggtg cagtaggtcg tcggctctgt tccgctgacg
     1561 aacacctcgc tgatgacggg gttgcacccg gcgcgcaggt tggccttcag ccccgtacgc
     1621 cgatcgacgg actgcaccga gatgttggcg ggctgcggaa aatcgccggc cggccgcagc
     1681 ggcagcgcct gttccatgaa ctcctgccag atcggtaggg cggcgatcgc gccggtctcc
     1741 cggctgccca ggctgcgcgg ctcgtcgaaa ccgacccaga cgccgacggc caggttcggt
     1801 gagtagccga cgaaccacgc gtcggtgttg tgatcggtgg tgccggtctt cccggccacg
     1861 ttggatccca accgcgacgc ggcccgtccc gtgccgtcgc tgatcacgcc cgagagcacg
     1921 cggttcatca ggtaggcgat ctcggggcgc acggcgtccc gaacctcggg ctcgatctgc
     1981 gcgaccagcg cgccctcgcg gttgtgcacg tcgcgcacga ggtgcggctc gaccagcacc
     2041 ccttggttcg cgaatgcacc gtaggccgcg gtcagctcga gcagcgtgac ctcgaacgcg
     2101 ccgagggcca gcgacgggta ggggcgcagg tcgccgctga tgccgagacg ccgtgcggtg
     2161 tcgatcaccg gcgagtaacc cacctcggtc agcagcttga ccgtggcgat gttggccgac
     2221 ttctccaggg cgctgcgtag cgtgaccgtc tcgtagtact cgttggtgta gttctccggc
     2281 tggtacggct cggggttgcg ccggtcgagg aagaccgtcg gttcgtcgac cagcgtgtgc
     2341 gccagcgtcc agccttcgct gagggcggcg gcgtagacga acggcttgaa cgccgagccg
     2401 gtctgccgcc gagcctgggt ggcgcggttg aactcactgc gctcgaagtc gaacccgccg
     2461 accatggcga ggattgcacc gctcgtcggc tcgaggacga cgatcgcggc ctcggccagc
     2521 ggttcctgtt cgagcgtcaa gcgcgcccgc ccctgctcgt cggtcgattc gacgcgcacc
     2581 cgaacgacgt cgcccacgtc gagcaacttg tccggacggg cgatcgcggt ccaggcgatt
     2641 ctctcgcggg tgaggttccc ctcgtagctg gcgacgcgga cacgagcccg ctcggaatcg
     2701 acatcgatca cgatcccgtc gtgcacttcc ccgggccgga tgccggcggc ccagtcggcc
     2761 gattgccaga tcagcgggtc ctccccgtcg ggcacgcgct gggtcacgcc gcgccagccc
     2821 tgcctgcggt cgagctggcg cagcccgacg tccatcgctc ggttggcgat ctgttgcaag
     2881 tcgggatcga gcgtcgtact gacgaccagg ccttccttgt acaggctgga gctgccgtac
     2941 tcggcttgca gccaacgccg cacgtcctcg acgaagtacg gcgccgggtc gtcggtgcgc
     3001 cgcagctgcg ccacctcgag cggctgtttc tgcgcctcgg cggcggcggc ctcgctgagc
     3061 agtccctcgt cggccatgcg ggacagcacg tagttgcgac gctcgatcga gcgttgcggg
     3121 ctacgcaacg gcgacagcgc ctccgggcgt tggatcagcc cggccagcgt cgccgactcg
     3181 accagcgaca tctcgcgggc gggtttgccg aaatagtagc gtgccgcggc ctcgatgccg
     3241 tagcgcccgt ggccggtgta gatctgattg cagtagaacc gaagaatctc ctgcttcgtg
     3301 tactggcgct cgatctcgag ggccagcacc acttcctgca gcttgcgccg gatcgtcttg
     3361 tcggggtgga gaaagaggtt gcgcgcgagc tgctgcgtca gcgtgctcgc gccctctttc
     3421 atccgcatgt gccgcagatc gctccacagc gcgcgcacga tgcccttgac gtcgataccg
     3481 gggtgccgat agaagctcga gtcctcggta gcgacgagcg cttggatgaa gacctgcggg
     3541 atgtcgggca gatcgatcag catccgccgc tcctcggcga acgaagcgat catcgagccg
     3601 tcgcgggcca tcacgcgcgt catcaccggc gggttgtagt cttcgaggcc gcgaacgtcc
     3661 ggcaggtcga accggacgaa ccggccgaag acgaggccgg ccaacgcggc accgagcacg
     3721 cagccggcca tgacgagata cggcccgcgc cgcaggtaga acggcgggtg cgcgtccggg
     3781 ctgcggggcg gtgggttccc gggcgccggg gggaactggc tcgggtgcat tctctccgcg
     3841 tctcctgcga gtcgcctccg atagcatagc tccggatcga ccctcggtgc acgtcccggc
     3901 tcgcgtggtt cgccggatgg cccacgcgat ccgggctatg cttgacgttt cacgagaggg
     3961 tgcgacgtgc agccgttcga gctcgtgtcg ggtttcgagc ccaccggcga ccagccggcc
     4021 gcgatcgatg cgctcgcccg aggtctgacc gacggcctgc ccgatcaggt gctactgggc
     4081 gtcaccggct cgggcaagac cttcaccatc gccaacctga tcgcccgcgc gcagcggccg
     4141 accctggtgc tggctcacaa caagacgctg gcggcccaac tctaccagga gttcagacgg
     4201 ctctttccgc ggaatgccgt cgagtacttc gtttcctact acgactacta ccagccggaa
     4261 gcctacgtcc cgcagtccga cacctacatc gagaaggagg cgacgatcaa cgaggagatc
     4321 gaccgcatgc ggcactcggc gacccgcgcg ttgttcgagc gccgcgacgt gctgatcgtc
     4381 gccagcgtct cctgcatcta cgggctcggc tcgcccgagg catatcacgg catgatgctg
     4441 ctgctcgagc gggggatgac gatcgaccgc gacgcggcgc tgcgcaagct ggtcgagatc
     4501 cagtacgagc gcaccggcgc cgacctccgc cgcggcgcct tccgcgtgcg cggcgatacc
     4561 gtcgagatct ggccctccta tgccgacgac gcctaccgca tcgagttctg gggcgaccag
     4621 atcgaagcga tcgcgcggat cgaccccgtc cgcggggtgc ggctcgagtc gctcgaccgg
     4681 catccggtct accccaactc gcactacgtc acgccgcgcg agacgctgat ccgcgcgatg
     4741 gagtcgatcc gcaccgagct cggggaacgg gtcgcggcgc tcgagcgcag cgggcggctg
     4801 ctcgaggcgc agcggctgaa gcagcggacg agcttcgatc tggagatgct gagcgagctc
     4861 ggctactgcc acggaatcga gaactactcg cgtcatctga ccggccgcca accgggcgaa
     4921 ccccctccga cgttgatgga ctacctgcac gacgattgcc tgctgatcgt cgacgagtca
     4981 cacgccaccc tgccgcaggt tcgcgggatg taccacggcg accggtcgcg caagctgacg
     5041 ctggtcgagc acggcttccg tcttccgtcg gcggtcgaca accggccgct gaccttcgac
     5101 gagttcgaac aacgtcgcgg tcaggcgatc tacgtctcgg cgacccccgg cgagctcgag
     5161 ctggcccgtg ccggcggcga gtacgtccaa caggtcgttc gtccgaccgg gctgctcgac
     5221 ccggcgaccg acgtgcgtcc ggtgaacggt caggtcgacg acctgttggc cgagatccgc
     5281 cggcggaccg accgcggtga gcgcaccctg gtcacgacgc tgaccaaacg gatggccgag
     5341 gatctgaccg actactaccg cgaactgggc gttcgggtcg agtacctgca ctcggaggtc
     5401 gtcacgctcg agcgagtgcg cattctgcgt gacctgcgcc gcggcgagtt cgacgtgctg
     5461 gtcggcgtca acctgctgcg ggaaggactg gatctcccgg aggtgtcgct cgtcgcgatc
     5521 ctcgacgcgg acaaggaggg ctatctccgc tccgagccgt cgctgatcca aacgatcggc
     5581 cgcgcggcga gacacgtcaa cggcagcgcg atcctctacg ccgatcgcgt caccggctcg
     5641 atgcggcggg cgctcgagga aacctcgcgc cggcgcggga tccagcaggc ctacaacgaa
     5701 cgacacggga tcacaccgga aaccgtgcgg cgcgcgatgg acgagctgat cggaactccg
     5761 atcgcggccg attactcgac ggtgccgctc gacgagccgc aggacgagcc cgtcttcgag
     5821 aatctggaca agctcgacga ggagatcggg cggttggaga agaagatgca t
//
LOCUS       DASPGN010000398        11481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1594904, whole genome shotgun sequence.
ACCESSION   DASPGN010000398 DASPGN010000000
VERSION     DASPGN010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11481
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1594904"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VD788_11520"
     CDS             complement(<1..311)
                     /locus_tag="VD788_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HXV76933.1"
                     /translation="MRKVSLFAIAVLLFAMPAFAGDKDQEKLPEGQTMANISISGMTC
                     GGCCSKVEAAVADLEGVVKVKADYEKKTAMVVYEKDKIDVDQIVEAINTKTSFKAEAG
                     KS"
     gene            416..1753
                     /locus_tag="VD788_11525"
     CDS             416..1753
                     /locus_tag="VD788_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HXV76934.1"
                     /translation="MAGGSARAAKKRILFGMFPAWGHVAPTVAIAQQLQDRGHDVAYA
                     VHPAISALLAEAGLETLAGLGWGRVAVRSQQHLQRGGFDLAWLVRVARGRPATTLVDD
                     LPRGVDELRVAMHRFWPDVIVNDLLFAPGSIAAELAGVPWAGSCPFALPQWDDDLPPY
                     GSGIPYGAPRDLRWRLLDLRTRRFRRRLDRSINRARRKLGAEALPHPFSRLSPWLDVA
                     YVTEAIEYPRPSLGRQVHFVGPSISRRRGDHRIPFPWDWLDGCPLVLVSLGTIFVHHE
                     RFLRSCAEAARDQPWQMVVRVGPAFDPTRGEAPPDNVRFVTELAQLALLPKCAAMLSH
                     AGMSSVLEALSEGVPLVLAPAAVEQPDTAARVVRAGAGIRIDLRRAGRDEIRTALRRV
                     LDEPAFRENARRIAGDFARCDGPVVAADLIERLADTRRPVLRPPGRGPTVYVR"
     gene            1843..2397
                     /locus_tag="VD788_11530"
     CDS             1843..2397
                     /locus_tag="VD788_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXV76935.1"
                     /translation="MKRKPWPTIGGALLAGLAWLAACDTTDRQRIERVLVDRAPWLDE
                     SARREIARAVTTVARSHEIDPWLLLAVIEVESTYRFDAHSRRGAVGLMQVKPLAATEV
                     AEAVGAEWTSHSDLLVPARNVAVGAAYLVQLKRRFRTWDGALTAYNRGPGRAGAAGPV
                     LTSRYAKRVLRVHASMLEQANGSP"
     gene            2455..3432
                     /locus_tag="VD788_11535"
     CDS             2455..3432
                     /locus_tag="VD788_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HXV76936.1"
                     /translation="MSRITGGIAVALVGRLALPAGDEARLRLASDAWPPFTDVAGETR
                     LALDLVHKALERAGVAAETTIVSWNEATAGLRDGEFDGSAAIWHDAERERRLLYSEPY
                     LENRLVLVGKNGSDVGAGDLPALAGKRIAVVDQYAYGAEVDDASGPLFVGGPSDQANL
                     ARLLAGEVDYMLVDELLIRYLVEHRQQETAQLLEIGRTPLVRRPLHFAVRRELAEAQT
                     IVAKFNAEIRQMLADGSYNVILALDWIRADVDGDGRPELVPRESRAGTTAPAHGYDIL
                     SSIPREPPVTALDRYWIDGRVYDGWEQVPDRYKTPPRAIDEPPPALLRF"
     gene            complement(3488..7273)
                     /locus_tag="VD788_11540"
     CDS             complement(3488..7273)
                     /locus_tag="VD788_11540"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXV76937.1"
                     /translation="MAVAVAVVVGLTGIQGAGLDAPATIIYDSYRVPTIVAETEHDAI
                     FLQGYLHAKDRLFQMDFQRRLFSGRVSELVGPAALPQDVQLRTLGLRRAADRSLLAQT
                     PEAMAWLQAYADGVNAYLADPSQPLPIEYASLEIDRGGIPPWTPTDTLVMAKGLSFGL
                     SFDLGDIDRTLALLNFLGVCEVLGCNGLQLFNDDLYRTAPFESVVSIPPANPPAAGSE
                     PDVPAEESLPGYMSDPDFAALVQGYRATIAEIPILEQTLASEPSAQGSNWWIVSGARS
                     DSGFPMIANDPHLALDNPATFYEVHLSVAGGINVTGTSFPGTPGVVLGCNDTICWGAS
                     VNAIDVTDVYNEVLLPLDPSQPTTPTHTLFGGVPEPLQFIPQLFLFNVIGDGVPNNTL
                     NAGIPATQGGVTLVVPRRNNGPIVNVSFNPASPTPLTGLSVQYVGWSATQEVECLRRF
                     ARAGSLQEFKDALQYFDVGSQNWSYADKHGNIAYYTSGELPIREDLQKNFFPAGLQPP
                     SLIRDGTNTNKHEWLPLANPQPNQALSTEILPFTEMPQIENPSAGYILNANNDPIGTT
                     LNNVSWDQFRPGFNGLLYLSSGYATGYRMGRLQRLFNEKLAGGGTLSLADFVEIQGNN
                     QLLDAEVLTPYILQAFANATSSGAPPELAALTADPRLEPAIDRLAAWDFSTPTGIDQG
                     FDPGDNPAAPGPPDQAEIDASVASTIYAAWRSHVVRRVIDTTLASLPVPLDDFAPGSD
                     QAMKALRRFLDNFPINGGVGSSLINFFVVPGVDEPPVARDIILLQSLLAGLDLLASDE
                     MAPAFGNSTDLADYRWGKLHRLVLDHPLGSPLLSIPPAGSLQNVSPQLRGFARAGGMG
                     ALDASSHSVRASRLNDFMFGSGPARRMISTMTPDGPEMLEVIPGGESGMPGSPQQVDQ
                     LVLWLVNAYKPLPVSLEDVEANADETVTIVCDGGDEPTCRLAPVISCLAPTLDADAQT
                     CTASAACGVVASCLDPGGAMVGLSCDTTGPFGLGASAVNVQCTNGVDAVSTSCPVTVV
                     DQTPPTISFELMPATLWPPNHHLVDVTASVVAEDSCGAASVSLASVGSSELDDADGNG
                     DGSTINDIQGADLGMTDTALSLRAERAGHGDGRVYTVSYSATDAAGNTTTASSAVMVP
                     HDQGGVVDPLDLSLEQTMHGTLARWGEVEEAQHYNVIRSTLGDLRADRSKYSLGPVTC
                     IRGGVQVLDTRGHEDDDVPAPGEAFIYMVEFHDGMSRSSYGTESAAKPRQVFSGDCE"
     gene            complement(7318..8892)
                     /locus_tag="VD788_11545"
     CDS             complement(7318..8892)
                     /locus_tag="VD788_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76938.1"
                     /translation="MIHVHRGPSPWRFCHRVMLVAGFAALAATPFATAHSPTVDASLR
                     DWCVGAFSNTAPGGGRQEDTGIALTCGNCSANSGLACEQSSDCPSGQSCVNVTSRTEI
                     AWWDDRTDGAVNDLATVAITQDMTNLYIAAELWVDPDPVSLPFGQIAIDHAPGGLGSW
                     HDPSGMLRRPGHCSLSTDRACARDEDCHFCQLSTEPLPSTRVRACGSGCDPDINDVCL
                     TNETCVDLGAGGPQSAAGFHASPIGFPDHLVLFDFSLWLIGAGDAVLLMQPGSVVDPN
                     PWDPVLGCAPDFAGDTTACDFPPAVNPGASGGSGGPPGSVEVAIPWSAFGCTGCPGAC
                     SCPGFGPGQPFRFSMIVARGTLTLDFAPDGALEDVVSEAVAGTTTTSTNSCAGFGIGN
                     TSCETDGSLDAFVPRTPVLPHETPPGGRVVQLTMSKAAGSSINLDWFPSCSADDVDYE
                     VYEGMLGNWYSHQPVVGFCMTGGTSATFDPSAGSSYYLVVPTNGVTEGSYGTSTAGER
                     PVGAMPCAAQVLGSCP"
     gene            complement(9029..>11481)
                     /locus_tag="VD788_11550"
     CDS             complement(9029..>11481)
                     /locus_tag="VD788_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV76939.1"
                     /translation="ILINELKVTQVTDAAGKFDFETVPPGTYNLSFTLADNSLTRTVI
                     VTAGKTMKLELEVDWDLGVSEKVTVTAAARAAKIVDAPAAVTAIPEEQIEQQASTGQV
                     PKVLEFTPGAEVTQSGLYDFNFNTRGFNSSLNRRVSTYIDGRDVGVVLLGAQEWAAIS
                     GGLDDVAELEFIRGPSAALYGANASSGVVNITTKAPRDSLGSLVRLTGGDLDTRSIDF
                     RRADRLGGGWYTKVLLGAKDSGDFTISRSPDLSPQPEYSEYCFQTGDSDCLPREKPLF
                     ISQDNEIRYGSLRVDKYLSDKAVLTLEGGLSDISGPVFQTGIGRVQNLDSDRPFYRFN
                     FSNPNWNVLAHYSERMGDQANLNEDLIVNYELITDTKRYGIEAQGNWSLADDRVRFVV
                     GAAHTEERVDTTNPETGEQTVVYEPIEMNRQAVFSQVDWTASRHFKLVFAGRVDRSTL
                     HDTQFSPKAALVYSINPKNSIRLTYNRAVQVANYSEFFLHTRIAPFPIGGFVRTICEA
                     PILDEPVDCGIDENFIPVLAVGNDDLELEKTAAWEIGYSGLLGRKVFLTIDYYNAENT
                     DFITDLIQQVGTPLGNVDGCIDNSLQPSDDPRECPVNQDYLPWISTPEAETTILIGTL
                     TVAQALRNAVENSVGGDLLGFRLAQDLDGSTVVVGRTYTNVGQVDTQGIDFGIQYFVT
                     PKLNLQASYSWFDFEIVDYGENAADFDMEDRADVEQILLPNSPTHKASVAMTLATGRW
                     TASAGARWVEGFRWSAGIFQGDVPSYTTGDVSASYAFNEIVRLGLNVANVADNEHHQT
                     FGGDLLTRRALLNLTLTW"
BASE COUNT         1783 a         3853 c         3955 g         1890 t
ORIGIN      
        1 gacttgccgg cctccgcctt gaagctggtc ttggtgttga tcgcctcgac gatctggtcg
       61 acgtcgatct tgtccttctc gtagacgacc atcgccgtct tcttctcgta gtcggccttg
      121 accttgacca cgccttcgag atcggcgacc gcggcttcga ccttcgagca gcagccgccg
      181 caggtcatcc cgctgatcga gatgttggcc atcgtctgcc cttcgggcag cttttcctga
      241 tccttgtcgc ccgcgaacgc gggcatcgcg aacagcagca cggcaatcgc gaatagcgag
      301 actttcctca tcgggtcctc cctgtagatg acgctcgtca tcggtcttct tctgaccctc
      361 ggattatagc gtgcgatcgg cgacgcgcta gcgcgcgccg gcatagaatc tgcggatggc
      421 gggtggcagc gcgcgagccg cgaaaaaacg cattctgttc gggatgttcc cggcttgggg
      481 tcacgtcgcg cccaccgtcg cgatcgcaca gcagttgcag gaccgggggc acgacgtggc
      541 ctatgccgtg cacccggcga tctccgcgct gcttgccgag gcggggctcg agacgctggc
      601 gggtctcggc tggggccgcg tcgcggtgcg cagccagcag cacctgcaac gcggcggctt
      661 cgacctcgcg tggttggtgc gggtggcgcg gggccgaccg gcgacgacgt tggtcgacga
      721 tctgccccgc ggcgtcgacg agctgagagt ggcgatgcat cgcttctggc ccgacgtgat
      781 cgtcaacgac ctgctgttcg cgccaggatc gatcgcggcc gaactcgccg gggtgccgtg
      841 ggccggaagc tgcccgttcg cgcttccgca gtgggacgac gacctgccgc cgtacgggtc
      901 cggtatcccg tacggcgcgc cgcgcgatct gcgctggcgc ctgctcgacc tccgcacgcg
      961 gcgattcaga cgccgcctcg accgttcgat caaccgcgcg cgacgcaagc tgggcgcgga
     1021 agcgctgccg catccgttca gccgcctgtc gccgtggctc gacgtggcct acgtcaccga
     1081 ggcgatcgag tacccgcgtc cgtcgctcgg gcgtcaggtt cacttcgtcg gtccgtcgat
     1141 cagccggcgg cgcggtgatc accgcatccc gttcccgtgg gactggctcg acgggtgccc
     1201 gctggtgctg gtcagcctgg gcacaatctt cgtgcaccac gagcgcttcc tcaggagctg
     1261 tgccgaggcg gcgcgcgatc agccgtggca gatggtcgtt cgcgtcggtc ccgccttcga
     1321 cccgacgcgc ggcgaggcgc caccggacaa cgtcaggttc gtcaccgagc tggcccagct
     1381 cgccctgctg ccgaagtgcg ccgccatgct gtcccacgcc gggatgagct cggtgctcga
     1441 ggcgttgagc gaaggggtcc cgctcgtcct cgccccggcc gccgtcgaac aacccgacac
     1501 ggccgcgcgg gtcgtgcgcg cgggcgcggg gatccggatc gatctccgcc gcgccggccg
     1561 cgacgagatc cgcaccgccc tccgtcgcgt gctcgacgaa cccgccttcc gcgagaacgc
     1621 gcgacggatc gccggcgact tcgcgcgctg cgacgggccg gtcgtggcgg ccgatctgat
     1681 cgaacgtctg gccgacacgc ggcggcccgt gctgcgccca ccgggccgag gaccgacggt
     1741 ctacgtgcgt tagtctcgcg gcgtccgccg cgccgtcaac agccggatca ccccacgccg
     1801 ggcgaacctc cgccggattc gcgtatgctc ggaccgacga cgatgaaacg gaaaccgtgg
     1861 ccgacgatcg gtggcgcgct gctcgcgggt ctcgcctggc tcgccgcctg cgatacgacg
     1921 gacagacaac ggatcgagcg agtgctcgtc gatcgcgcgc cctggctcga cgagagcgca
     1981 cggcgtgaga tcgcgagggc ggtcaccacg gtcgcccggt cccacgagat cgacccgtgg
     2041 ctgctgttgg ccgtgatcga ggtcgagagc acctaccgct tcgacgctca cagccgccgt
     2101 ggggcggtcg ggctgatgca ggtcaagccg ctcgccgcga ccgaggtggc ggaggcggtc
     2161 ggggccgaat ggaccagcca ctccgacctg ctggtgccgg cacgcaacgt cgccgtcggg
     2221 gccgcgtacc tcgtccagct caagcggcgg ttccgcacct gggatggagc gctgacggcc
     2281 tacaaccgcg ggccggggcg ggccggcgcc gccggtcccg tgctgacgtc gcgctatgcc
     2341 aagcgcgtgc tgcgcgtcca cgcgtcgatg ctcgagcagg cgaacggttc gccgtgagcc
     2401 ggtgtgttac cttgtcccgg gcagcgctgg tgtaaagggg ccgcgatggt cgcgttgtcc
     2461 aggatcaccg gagggatcgc ggtggcgctc gtcggtcgac tggcgctacc ggccggagac
     2521 gaagcgcggc tgcgcctcgc ctcggacgcc tggccgccgt tcaccgacgt cgccggtgag
     2581 acacgtttgg ccctcgacct cgtgcacaag gccctcgagc gtgcaggcgt tgccgccgag
     2641 acgaccatcg tgtcgtggaa cgaggcgacc gccgggctcc gtgacggcga gttcgacggg
     2701 agtgcggcga tctggcacga cgcggagcgc gagcgccggc tgctctactc cgaaccctac
     2761 ctcgagaatc ggctggtcct cgtcggcaag aacggaagcg acgtcggcgc cggcgacctg
     2821 cccgctctgg ccggcaagcg gatcgcggtc gtcgaccaat acgcctacgg cgccgaggtc
     2881 gacgacgcct cggggccgct gttcgtcggc ggccccagcg accaagcgaa tctggcgcgc
     2941 ctgctcgccg gcgaggtcga ctacatgctg gtcgacgagt tgttgattcg ctacctggtc
     3001 gagcaccggc agcaggaaac ggcgcaattg ctcgagatcg gacggacgcc gctcgtcaga
     3061 cgcccgctcc acttcgcggt ccgacgcgag ctggccgaag cgcagacgat cgtcgcgaaa
     3121 ttcaacgccg agatccggca gatgctggcc gacggcagct acaacgtgat cctcgcgctc
     3181 gactggattc gtgccgacgt cgatggcgac gggcggccgg aactggtgcc gcgggaatcc
     3241 cgcgccggca cgaccgcgcc ggcccacggc tacgacatcc tgtcgtcgat cccgcgcgag
     3301 cctccggtca cggcgctcga ccgctactgg atcgacgggc gcgtctacga cggctgggag
     3361 caggtgccgg atcgatacaa gaccccgccg cgcgcgatcg acgaacctcc gccggccctg
     3421 ctccgtttct agcggatcgg cgccgggcca cccgtcgacg ggcacccgcg cgcgatccga
     3481 cccgcaatca ctcacaatcg ccgctgaaca cctgccgcgg cttggccgcg ctctccgttc
     3541 cgtacgagct tcgcgacatg ccgtcgtgga actcgaccat gtagataaac gcttcacccg
     3601 gcgccgggac gtcgtcgtcc tcatgaccgc gcgtgtcgag tacctggacg ccgccgcgga
     3661 tgcaggtcac cggaccgagt gagtacttgc tgcggtcggc gcgcagatcg cccagcgtcg
     3721 agcggatgac gttgtagtgc tgcgcttcct ccacctcgcc ccagcgagcg agcgtgccat
     3781 gcatcgtctg ctcgagggac agatcgagcg gatcgacgac gccgccttgg tcgtgcggaa
     3841 ccatgaccgc cgagctggcc gtcgtcgtgt tgcccgccgc gtcggtcgcg gagtacgaca
     3901 ccgtgtagac gcgaccgtcg ccgtggcccg cgcgttccgc gcgcaacgac agtgcggtgt
     3961 cggtcatgcc caggtcggcg ccttggatgt cgttgatcgt gctgccgtcg ccgttgccgt
     4021 cggcatcgtc cagctcgctg ctgccgacgg aagccaggct gaccgacgcc gcgccgcagc
     4081 tatcctccgc gacgaccgac gccgtgacgt cgaccaagtg gtggttcggc ggccacaacg
     4141 tcgccggcat cagctcgaac gagatcgtcg gcggcgtctg gtcgacgacc gtcaccgggc
     4201 agctcgtcga gaccgcgtcc acgccgttcg tgcactgcac gttgaccgcg gaagcgccca
     4261 aaccgaacgg gccggtcgtg tcacacgaca ggccgaccat cgctccaccg ggatcgagac
     4321 acgaggccac caccccgcac gcggcggacg cggtgcaggt ctgggcatcg gcatcgagcg
     4381 tcggggcgag acagctgatg accggtgcga gccggcaggt cggctcgtcg ccgccgtcgc
     4441 agacgatcgt caccgtttcg tcggcgttcg cctcgacgtc ctcgagcgac accggcagcg
     4501 gcttgtaagc gttgaccagc cacagcacga gctgatcgac ctgctgcggg cttcccggca
     4561 ttccgctttc gccacccggg atcacctcca acatctccgg tccgtcggga gtcatcgtcg
     4621 agatcatccg ccgggcgggg ccgctgccga acatgaagtc gttgagccgg ctggcgcgca
     4681 cggaatgcga cgaagcgtcg agcgcgccca tcccgccggc acgggcgaag ccgcgcaact
     4741 gcggcgagac gttttgcaac gagccggccg gcgggatgct caagagcggc gagcccaatg
     4801 ggtgatcgag cacgaggcga tgcagcttgc cccagcggta gtccgccaga tcggtcgaat
     4861 tgccgaaggc cggtgccatc tcgtccgagg cgagcaggtc gaggccggcg agcagactct
     4921 gcagcaggat gatgtcccgt gcgaccggcg gttcgtcgac gcccggcacg acgaaaaagt
     4981 tgatcagcga cgagccgacc ccgccgttga tcgggaagtt gtcgaggaag cgccgcagtg
     5041 ccttcatcgc ctggtcgctc cccggtgcga agtcgtcgag cggaaccggc agcgacgcca
     5101 gcgtggtgtc gatcacgcgg cgcacgacgt ggctgcgcca ggcggcgtag atcgtcgaag
     5161 cgacgctggc gtcgatctcg gcctggtcgg gcggaccggg cgcggccgga ttgtcgcccg
     5221 gatcgaagcc ctggtcgatt ccggtcggcg tgctgaagtc ccacgccgcc aggcggtcga
     5281 tcgctggctc gaggcgcggg tcggccgtca gcgccgcgag ctcgggtggc gcaccgctcg
     5341 acgtcgcgtt ggcgaacgcc tgaaggatgt agggtgtcag cacctcggcg tcgagcagct
     5401 ggttgttgcc ctggatctcg acgaagtcgg cgagcgacag cgtgcccccg ccggcgagct
     5461 tctcgttgaa cagacgctgc aggcgtccca tccggtagcc ggtggcataa cccgagctga
     5521 ggtagagcaa accgttgaac cccggccgga attgatccca cgacacgttg ttcaacgtcg
     5581 tgccgatcgg gtcgttgttc gcgttgagga tgtagccggc gctcgggttc tcgatctgcg
     5641 gcatctcggt gaagggcagg atctcggtcg acagtgcctg gttcggttgc gggttggcga
     5701 gcggcagcca ctcgtgcttg ttggtattcg tcccgtcacg gatcaaagac ggcggctgca
     5761 gacccgccgg gaagaagttt ttctgcaggt cctcgcggat cggcagctcg ccgctcgtgt
     5821 agtaggcgat gttgccgtgc ttgtcggcgt aggaccagtt ctgcgagccg acgtcgaagt
     5881 actgcagggc gtctttgaat tcctgcaggc tgccggcgcg ggcgaaccgg cgcaggcact
     5941 cgacctcctg ggtcgcgctc cagccgacgt actgcacgct gagaccggtc agcggcgtcg
     6001 gcgaggccgg gttgaacgac acgttgacga tcgggccgtt gttgcgccgg ggaacgacga
     6061 gggtcacgcc gccctgggtc gccgggatcc ccgcgttgag cgtgttgttc ggcacaccgt
     6121 cgccgatgac gttgaacagg aacagctgcg ggatgaactg cagcggctcg ggaacgccgc
     6181 cgaacagcgt gtgagtcggc gtcgtcggct ggctcgggtc gagcggcagc aatacctcgt
     6241 tgtagacgtc ggtcacgtcg atcgcgttga ccgaggcacc ccagcagatc gtatcgttgc
     6301 agccgagcac cacgcccggc gtgccgggga agctggtacc ggtgacgttg attcccccgg
     6361 cgacgctgag gtgcacctcg tagaacgtgg ccgggttgtc gagcgccaag tgcgggtcgt
     6421 tggcgatcat cggaaaaccg ctgtcgctgc gggcgccgga gacgatccac cagttgcttc
     6481 cctgcgccga cggctcggac gcgagcgttt gctcgaggat cgggatctcg gcgatcgtgg
     6541 cgcgatagcc ctggaccaac gccgcgaagt ccgggtcgga catgtaaccg ggcagcgact
     6601 cctccgccgg gacgtcgggc tcggagcccg cggccggcgg gttggccggt gggatcgaca
     6661 caaccgactc gaacggcgcc gtgcggtaca ggtcgtcgtt gaacagttgg agcccgttgc
     6721 agccgagcac ttcgcagacg ccgaggaagt tcaacaaggc gagcgtgcga tcgatgtcgc
     6781 ccagatcgaa cgagagaccg aaactcaggc ccttcgccat caccaacgtg tcggtcggcg
     6841 tccacggcgg aatgccgccg cgatcgatct cgagcgaggc gtactcgatc ggtagcggct
     6901 gcgatggatc ggccaggtag gcgttgacgc cgtcggcata ggcctgcagc cacgccatcg
     6961 cctcgggagt ttgcgcgagc aggctgcggt cggcggcgcg tcgcaggccg agcgtgcgca
     7021 gctggacgtc ctgcggcaac gccgcggggc cgacgagctc cgacacgcgc ccggaaaaca
     7081 gccggcgctg gaagtccatc tggaacaggc gatccttggc atgcaggtag ccctgcaaga
     7141 agatcgcgtc gtgctcggtc tcggcgacga tcgtcgggac gcgatacgag tcatagatga
     7201 tcgtcgccgg agcatcgagc ccggcgccct ggatccccgt caggccgacc acaacggcga
     7261 cggcgacggc cactgaagcc ttgtctctgt aagtcatgat cccccccggg tcgaactcta
     7321 cggacagctg cccaagactt gggccgcaca cggcatcgcg ccgacgggcc gctcgcccgc
     7381 ggtgctggtg ccgtaggagc cttcggtgac tccgttggtc ggaacgacga ggtagtagct
     7441 gctccccgcc gacgggtcga acgtcgcact cgtgcccccg gtcatgcaga agccgacgac
     7501 cggctggtgg ctgtaccagt tgccgagcat tccctcgtag acctcgtagt cgacgtcgtc
     7561 ggccgagcag gacgggaacc agtcgaggtt gatcgacgac ccggccgctt tggacatcgt
     7621 cagctgcacg acgcgtccgc cgggcggggt ctcgtgcggc aagaccggcg tgcgaggaac
     7681 gaacgcgtcg aggctgccgt cggtctcgca gctcgtgttg ccgatcccga agccggcgca
     7741 gctgttggtc gacgtggtcg tcgtgccggc gaccgcttcc gagacgacgt cctcgagcgc
     7801 gccgtcgggc gcgaagtcga gcgtcaacgt cccgcgcgcg acgatcatcg agaagcggaa
     7861 cggctgcccc ggcccgaacc ccgggcagct gcacgctccc ggacaaccgg tgcaaccgaa
     7921 cgcgctccac ggaatcgcga cctcgaccga ccccggaggg ccgcccgagc cgccgctcgc
     7981 gcccgggttt accgcgggcg gaaagtcgca ggccgtcgta tcgccggcga aatccggcgc
     8041 gcaaccgagt accggatccc acgggttcgg gtcgacgacg ctccccggct gcatcagcaa
     8101 caccgcgtct cccgccccga tcaaccacag gctgaaatcg aacagcacga ggtggtcggg
     8161 gaagccgatc ggactggcgt ggaacccggc ggcgctttgc gggccgcccg cgcccaagtc
     8221 cacgcaggtt tcgttggtca ggcagacgtc gttgatgtcc gggtcgcacc ctgaaccgca
     8281 ggcccgcacg cgcgtcgacg gcaacggctc ggtcgacagc tggcagaagt gacagtcttc
     8341 atcccgcgcg cacgcgcggt cggtcgaaag gctgcagtga cccggcctgc ggagcatccc
     8401 gctcggatcg tgccagctac cgagcccgcc cggcgcgtga tcgatcgcga tctggccgaa
     8461 cgggagcgag accggatctg ggtcgaccca cagttcggcg gcgatgtaca ggttggtcat
     8521 gtcctgggtg atggcgaccg tcgccaggtc gttgacggca ccgtcggtcc gatcgtccca
     8581 ccacgcgatc tcggtccggc tggtgacgtt cacgcagctc tggcccgagg ggcagtcgct
     8641 cgactgctcg caggccagac cggaattggc cgaacagttg ccgcaggtca acgcgatgcc
     8701 ggtgtcttcc tgacggccgc cgcccggcgc cgtgttcgag aacgccccga cgcaccagtc
     8761 gcgaagcgac gcgtcgacgg tcgggctgtg ggccgtcgcg aacggcgtcg ccgccagcgc
     8821 cgcgaacccc gccacgagca tgacacgatg acagaatctc cacgggctcg gccctcgatg
     8881 gacgtggatc atgactgcct cccctggcgt tggatgcccg gattataggt cgattcccgg
     8941 ttcgaagcag cgcaattcgg acccgccggc gggcgaaaca gaagatttgg agttatcgcg
     9001 acggcaggcc ggcggttcgg ccctgcgatc accacgtcag cgtcaggttg agcaacgcac
     9061 ggcgcgtgag caggtcgccg ccgaaggtct ggtggtgctc gttgtcggcc acgttggcga
     9121 cgttgagccc gagtcggacg atctcgttga aggcgtagct cgccgacacg tcgccggtcg
     9181 tgtagctcgg cacgtctccc tggaagatgc cggccgacca gcggaaaccc tcgacccatc
     9241 gcgcgccggc gcttgcggtc cagcgtcccg tggccaacgt catcgcgacg ctcgccttgt
     9301 gcgtcggcga gttgggcagc aggatctgct cgacatcggc gcggtcctcc atgtcgaagt
     9361 cggccgcgtt ctcgccgtag tcgacgatct cgaagtcgaa ccacgagtag ctcgcctgca
     9421 ggttcagctt cggtgtgacg aagtactgga tgccgaagtc gatcccctgc gtgtcgacct
     9481 ggccgacgtt ggtgtacgtg cggccgacga cgacggtcga cccgtcgaga tcctgcgcca
     9541 gccggaaccc gagcagatcg ccgccgaccg agttctcgac ggcattgcgc agcgcctgcg
     9601 cgacggtcag cgtcccgatc aggatcgtgg tctcggcctc cggcgtgctg atccatggca
     9661 agtagtcctg gttgaccggg cactcgcgcg ggtcgtccga cggctgcagg ctgttgtcga
     9721 tgcagccgtc gacgttgccc agcggcgtgc cgacctgctg gatcagatcg gtgatgaaat
     9781 cggtgttttc cgcgttgtag tagtcgatgg tgaggaacac cttccggccc agcaagccgc
     9841 tgtagccgat ctcccacgcc gcggtcttct ccagctccag gtcgtcgttg ccgaccgcga
     9901 gcaccggaat gaagttctcg tcgatcccgc agtcgacagg ctcatcgaga atcggcgcct
     9961 cgcagatcgt ccggacgaac ccgccgatcg ggaacggcgc gatgcgcgtg tgcagaaaga
    10021 actccgagta gttcgcgacc tggaccgcgc ggttgtaagt caggcggatc gagttcttcg
    10081 ggttgatgct gtagaccagc gccgccttgg gtgaaaactg ggtgtcgtgc agggtgctgc
    10141 ggtcgacccg gccggcgaac accagcttga agtggcggct cgccgtccag tcgacctggg
    10201 agaagacggc ctgccggttc atctcgatcg gctcgtagac caccgtttgc tcgcccgttt
    10261 cggggttcgt cgtgtcgacc cgctcttcgg tgtgggcggc gccgacgacg aaccgcaccc
    10321 ggtcgtcggc caagctccag ttgccctggg cctcgatgcc gtagcgcttc gtgtcggtga
    10381 tcagctcgta gttgacgatc aggtcttcgt tgagattggc ctggtcgccc attcgctcgg
    10441 aatagtgggc cagcacgttc cagttcgggt tggagaagtt gaaacggtag aacggccggt
    10501 cggaatcgag gttctgcacg cggccgatgc cggtctggaa caccggaccc gaaatgtcgg
    10561 acagaccgcc ctcgagcgtg agcaccgcct tgtcggacag gtacttgtcg acacgcaacg
    10621 agccgtaacg gatctcgttg tcctgcgaga tgaacagcgg cttctcgcgc ggtaggcagt
    10681 cggaatcgcc ggtctggaag cagtactccg agtactccgg ttgcggactg agatccggac
    10741 tgcgcgagat ggtgaagtcg cccgagtctt tcgcgccgag cagcaccttg gtgtaccacc
    10801 ctccgcccaa gcggtcggcc cggcggaaat cgatgctgcg cgtgtcgagg tctccgccgg
    10861 tgagacggac caggctgccc aggctgtccc gcggtgcctt ggtcgtgatg ttgacgacgc
    10921 cgctcgatgc gttcgcgccg tacagcgcgg ccgacggccc gcggatgaac tcgagctcgg
    10981 cgacgtcgtc caggccaccg gagatcgccg cccactcctg ggcgccgagc agcacgacac
    11041 cgacgtcacg accgtcgatg taggtcgaga cgcgacggtt gagcgagctg ttgaagccgc
    11101 gtgtgttgaa gttgaaatcg tacaacccgc tctgcgtgac ctcggctccg ggcgtgaact
    11161 ccagcacctt cggcacctga ccggtcgacg cctgctgctc gatctgctct tcgggaatcg
    11221 cggtgaccgc cgccggcgca tcgacgatct tggccgcgcg cgcggcggcg gtgacggtca
    11281 ccttctccga cacgccgaga tcccagtcga cctccagctc cagtttcatc gtcttgcccg
    11341 cggtgacgat gaccgtgcgg gtcaggctgt tgtcggcgag cgtgaagctc aggttgtacg
    11401 tcccgggcgg aaccgtctcg aagtcgaact tgccggcggc gtcggtgacc tgggtcacct
    11461 tcagctcgtt gatcaggatc a
//
LOCUS       DASPGN010000399         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1359420, whole genome shotgun sequence.
ACCESSION   DASPGN010000399 DASPGN010000000
VERSION     DASPGN010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359420"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VD788_11555"
     CDS             <1..1095
                     /locus_tag="VD788_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76940.1"
                     /translation="DALASIAASERALDTTRPDLVVLSHAVNFQFGSLAWLAARRGIP
                     TVLAQGVYGVSRFVKLLQPEHLYETIDRPAATDLARLSRARAERLAAAGRAYLEARWS
                     GRTDDLGALYAFRRATETPTRERLAARFGWDAERPIVAVYASNWFDFPHPCGMTQFRD
                     FLDWMRATLDAAVDNRRVNWLFKAHPCDAWYGGVTLSDLVPPLDTHPHLRLVPVEWNG
                     AALLRAVDALVTVHGTAGVEFAAAGKPVLVADRGWYHDAGFVRWSRSRAEYVEALATD
                     WWKDLDVEATTRHAQVFAGWYFARPAWQDGLVMEDDPRQGEIYRTAPRLLTEHPQALA
                     RELATIREWFASPHRHYHTYKMSVADEFAF"
     gene            1079..>2542
                     /locus_tag="VD788_11560"
     CDS             1079..>2542
                     /locus_tag="VD788_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV76941.1"
                     /translation="MSSRSSVVERALGWLRASGTALTTAKEWRAFVDGMRAGLQLLSA
                     AERRRAGALSLAILVGSALEIVALAAVLPFINVVIQPASVNTNGLLRRVYELSGATDV
                     SEFVFLLGGGVIAVMLFSAAAGWALVYTQNRYAAACQTRLATHLLERALRAPYVWFLS
                     RNSTTLSRLVYDDVVIWSRAFVHRLIMMVEDVLVVIMAAALVLALSPRTGIAVVVVMA
                     ILGYVSVSLTRPLLSRLARSKREGLDAALLLAHQAFAGIKDVKLTSREHHFRALFHQT
                     YTTVADSHAALNVWQETPTRVLRVLAQLTLVALALVFWRMGIGSGQIATQLALLVIVT
                     TKVVPAVSALSTAVNNLANALPHIEAIHQALRSIDAETALVVTATGPGRAVGSWRTVR
                     FDAVSYRYPGARECALSDVSLDMSRRGSYGIVGRSAAGKSTLVDLLIRLLEPTEGCIW
                     IDGDPAPALDRRDWQRRIGYVPQAPFIADDTLRANVAF"
BASE COUNT          340 a          881 c          889 g          432 t
ORIGIN      
        1 gatgcgctgg ccagcatcgc cgcctcggag cgcgcgctgg acacgacccg tcccgacctg
       61 gtcgtgctca gccacgcggt gaacttccag ttcggttcgc tggcgtggct ggcggcccgg
      121 cgcggtattc cgactgttct cgcgcagggc gtctacggtg tctcgcgctt cgtgaagctc
      181 ctgcagccgg agcacctgta cgagacgatc gaccgccccg cggcgacaga cctcgcgcgg
      241 ctgtcccgcg cacgtgccga gcgactggcg gcggcgggtc gtgcctatct cgaggcgcgc
      301 tggtcgggcc gcaccgacga cctgggggcg ctgtacgcgt ttcgacgggc caccgaaacg
      361 ccgacacgcg agcggctggc cgcgcggttc ggctgggacg ccgagcgtcc gatcgtcgcc
      421 gtgtatgcgt cgaactggtt cgacttcccg cacccgtgcg ggatgacgca gttccgcgat
      481 ttcctcgact ggatgcgcgc cacgctcgat gccgccgtcg acaaccggcg cgtgaactgg
      541 ctgttcaagg cgcatccgtg cgacgcgtgg tatggcggcg tgacgctgtc cgacctcgtg
      601 ccgcccctcg acacgcatcc ccacctgcga ctggtgccgg tggaatggaa cggtgcggcg
      661 ctcttgcgtg ccgttgacgc cctcgtcacc gtccacggca ccgcgggcgt ggagttcgcg
      721 gctgccggca agcccgtcct cgtcgccgac cgcggttggt atcacgacgc ggggttcgtg
      781 cgatggtcgc gctcgcgcgc cgagtacgtc gaggcgctcg ccaccgactg gtggaaagac
      841 ctcgacgtcg aggcgacgac acgccacgcg caggtgttcg cgggctggta cttcgcgcga
      901 ccggcgtggc aggacgggct cgtgatggaa gacgacccga gacagggaga gatctaccgg
      961 actgcgccgc ggctgctcac cgagcatccg caggcgctcg cgcgagagct ggcgaccatc
     1021 cgggagtggt tcgcgtcccc gcatcggcac taccacacct acaagatgag cgtggccgat
     1081 gagttcgcgt tctagcgtgg tcgagcgcgc gctcgggtgg ctgcgcgcga gcggtaccgc
     1141 cctgacgacg gcgaaggagt ggcgtgcctt cgtggacggg atgcgtgccg ggttgcagct
     1201 gttgtcggcc gcggagcgaa gacgcgcggg cgcgctcagc ctcgccatac tcgtggggag
     1261 cgcgctcgag atcgtcgcgc tggcggcggt gctgccgttc atcaacgtcg tgatccagcc
     1321 ggcatcggtc aatacgaacg gcctcctgcg ccgcgtctac gagctctccg gcgcgacgga
     1381 cgtttccgag ttcgtgttcc tgctcggcgg cggtgtgatt gccgtcatgc tgttcagcgc
     1441 cgccgccggc tgggccctgg tctacacgca gaaccgttac gccgcggcct gccagacgcg
     1501 cctcgccacc catctcctcg aacgcgctct ccgggcgccg tacgtctggt tcctgtcgcg
     1561 caattcgacg acgctgtcgc ggctcgtcta cgacgatgtc gtcatctgga gccgcgcgtt
     1621 cgtccatcgg ctgatcatga tggtcgagga cgtgctcgtg gtcatcatgg cggccgcgct
     1681 ggtgctggcg ctctcaccgc ggacgggtat cgcggtcgtc gtggtcatgg cgatcctggg
     1741 ctacgtgtcg gtgtcgctga cgcgcccgct gttgtcccgg ctcgcgcgga gcaagcgtga
     1801 gggcctcgat gccgcgctgt tgctcgccca ccaggcgttc gccggcatca aggacgtgaa
     1861 gctcacgagc cgcgagcacc actttcgtgc cctgttccac cagacgtaca cgaccgtcgc
     1921 cgactcgcat gccgccctca acgtgtggca ggagacgccg acacgagtgc tgcgggtgct
     1981 cgcccagctc accctggtgg ccctcgcgct cgtgttctgg cgcatgggga tcggcagcgg
     2041 tcagatcgcg acgcagctgg cgctgctcgt catcgtcacg accaaggtcg ttccggcggt
     2101 gagcgcgctg tcgaccgccg tcaacaacct ggcgaacgcg ctcccgcata tcgaggccat
     2161 ccaccaggcg ctgcggtcca tcgacgccga gacggcgctg gtcgtcacgg cgacgggccc
     2221 cggcagggcg gtcggctcgt ggcgcacggt acgcttcgac gccgtctcgt accgttatcc
     2281 gggcgcgagg gagtgcgcgc tcagcgacgt gtcgctcgac atgagccgtc gcgggtccta
     2341 cggcatcgtc ggccgctcgg cggccgggaa gagcacactc gtggaccttc tcatccggct
     2401 cctcgagccg accgagggat gcatctggat cgacggggac ccggcgccgg cgctcgatcg
     2461 acgggactgg cagcgccgca tcgggtacgt tccgcaggca cccttcatcg ccgacgacac
     2521 gctgcgggcc aacgtggcct tc
//
LOCUS       DASPGN010000400        14234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_189583, whole genome shotgun sequence.
ACCESSION   DASPGN010000400 DASPGN010000000
VERSION     DASPGN010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 14234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14234
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189583"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VD788_11565"
     CDS             complement(<1..572)
                     /locus_tag="VD788_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXV76942.1"
                     /translation="MEIAANIPDGPIEKKWDRHRFELKLVNPANKRRHGVIVVGAGLA
                     GASAAATMAELGYRVRCLCYHDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFFDTIK
                     GGDFRSREANVYRLAQVSVNIIDQCVAQGVPFAREYGGMLANRSFGGAQVARTFYARG
                     QTGQQLLLGAYSSLMRQVHSGQVKMFARRE"
     gene            complement(589..1266)
                     /locus_tag="VD788_11570"
     CDS             complement(589..1266)
                     /locus_tag="VD788_11570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HXV76943.1"
                     /translation="MRDVLHLTQSSVGKKVLMAATGIVLAGFVFVHMLGNLKIYQGPE
                     KYDRYAEFLRTVGAPVFGHGQVLWIARFVLLFCVGVHVYAATDLYLRCRRARSARYRK
                     FDDLSFSRASRTMRWGGVLLVAFVVYHLLHLTFGTAHPDFVAGSAYRNVVAGFKQWPV
                     SAAYVAAMIPLGLHLYHGMWSTTQTLALEGERVKTWRRPLAAALALAIVIGNVSIPVA
                     VLIGVVR"
     gene            1423..4404
                     /locus_tag="VD788_11575"
     CDS             1423..4404
                     /locus_tag="VD788_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXV76944.1"
                     /translation="MSDGSGTRFVDRLLDHPSLAPMVVHHRRIERVPAVLAQPRDPLP
                     PAIEAALTTAGLGGLYRHQAEGIDRLRERRDVLLVTPTASGKTLLFSVAVLETALADP
                     SSRALLLYPTKALAQDQLAGFRALAGGVGALRPPSFEIYDGDTPQARRRHIRSDPPSA
                     LITNPDMLHMGILAHHHDWAAFLRGLRWVVLDELHVYRGMFGAHVHHILARLERLCAH
                     YGARPQFIAASATVGNPAAFARTLVGREFVVIDRSGAPASARDVVFVNPIGVSPYTVA
                     VRVVAEAIRDRRRTIAFAKARRVTELLHSWLVQHEPEFRSRVAPYRAGYLPEERRAIE
                     GRLFRGELSAILSTSALELGIDVGALDVCVLVGYPGSLISSWQRIGRVGRRRDGGAVV
                     LIAMPDALDQYVVRHPDLFFGGRFENAVLDPWNRQIAGRHLVCAAAERPLAAGEYDRA
                     GQSGRQLIDELVRDGRLVQDAAGTRFFSFKRRPQREVSVRAAGEPFVIVDTAGKLLGT
                     IDGQRVYHECHAGAIYLHGGRSFLVRALDADRRRVTADRANVDYYTVVLGDKETAILE
                     RLDARRLGDFPVGLGRLEVTVRIRGYQKKRLFDGEPIAQHPLDVPPLVFETVGLWIEL
                     PAELPAAYAARELHFMGGIHAVEHAMIGLFPLLAIADRDDVGGISYTGHPQLGAPAVF
                     VYDGLAGGAGLAEQGFREIEHLVGRTLELVRSCDCEDGCPACIQSPKCGNGNKPLDKA
                     AALLTLRLLGGEEALASLGVEAAAEGAERAIPPAPRLDPAPIESLATGGTARRHGITR
                     SGNLEPARALDVRPSPPIAQRTLIFDLETQRGADEVGGWAHVERMGLALAVVYDTARE
                     VFRTYFETDVDRLLLDLVMADRVVGFNVDRFDMRVLAGYTDWDLSRVRTVDMLGLIHR
                     RLGFRVSLEHLCEANLGESKVGDGLQSLRWWKDGRIDLIEQYCKRDVELTRRLWDLGQ
                     RRGYLLYRDHEQRTVRVPVEW"
     gene            4473..5147
                     /locus_tag="VD788_11580"
     CDS             4473..5147
                     /locus_tag="VD788_11580"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HXV76945.1"
                     /translation="MGRPWAAVSGLVLAGLFGACGEPSTGMMVSIPPPDGWREQLEGS
                     RSDKDEFFKTDPGSPLLPAETAAFDGLDYWEPNADYYHVGRVNLYLEPQKFDVVTTSG
                     VIRPCAKVGWVGFRQGGQDHKLQVYRLLDQAPQQGGADYFLPFMDETTGEETYPAGRY
                     VDLVGPEGGPYVLDFNTASNPWCAYGDVDRFVCPVTPPENRLPVRIEAGERGYRQSPR
                     AEDGAG"
     gene            5156..5581
                     /locus_tag="VD788_11585"
     CDS             5156..5581
                     /locus_tag="VD788_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76946.1"
                     /translation="MAPLDAGRADGRAVVARARASATALVATVLAATAASSLLPSVRS
                     LDVLVGPAAAIGAIGPAIGYRRYLLARDRRGSYLRAVVSALAVTGVAALFGAAVHGVT
                     GELLPLIGPFMHVLLAAVIWPADERLSAFSGAPSGPGKS"
     gene            5586..6128
                     /locus_tag="VD788_11590"
     CDS             5586..6128
                     /locus_tag="VD788_11590"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXV76947.1"
                     /translation="MAPPVGSRTVFLVGFMCCGKTTVGRRLAERRGGSFIDTDERIAR
                     HTGRTIEAILAGPGEEELRRLEIDCLRSLPQAGATVVGTGGGAYSRYEVRRSLACRGL
                     TVWLDVSLGEARGRRKADATPRPLWSDSDDAVAFRAFFERRRAVYAMAEARVDADRAP
                     DEVVRAVERLLDEPESHFFH"
     gene            6211..6480
                     /locus_tag="VD788_11595"
     CDS             6211..6480
                     /locus_tag="VD788_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXV76948.1"
                     /translation="MTRTELIDDLANRTGTEKKDVKSFLEHLTSLIEDEIKKGGEVPL
                     KGLGKFRVQNRKARVGRNPLTGAEIQIPAKTVVKFSVAKSLKDLA"
     gene            complement(6577..7104)
                     /gene="purE"
                     /locus_tag="VD788_11600"
     CDS             complement(6577..7104)
                     /gene="purE"
                     /locus_tag="VD788_11600"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HXV76949.1"
                     /translation="MTERTRARVSVLMGSDSDLPVMAEAIKVLEQLEVPYEMHVTSAH
                     RSPERTTRIVATAPDRGVAVFIVGAGAAAHLAGVVAAHSTRPVLGVPLASSDLNGLDA
                     LLATVQMPAGVPVGTLAIGKAGAANAGWLAASILATADPGLAAKLATERARMADAIEA
                     KSERAKGKLRSLVGD"
     gene            complement(7097..8410)
                     /gene="purD"
                     /locus_tag="VD788_11605"
     CDS             complement(7097..8410)
                     /gene="purD"
                     /locus_tag="VD788_11605"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HXV76950.1"
                     /translation="MRVLLVGGGGREHALAWALGRSPQLDELVVAPGNAGIADDHRTV
                     AIAADDVSALVEHATSGAYDLVVVGPEAPLVGGLADRLGEAGVAVFGPTRRAAELEAS
                     KVFAKRFMTRHRIPTADYRVFDGAAAARDYLGARGRPYPLVVKADGLAAGKGVSICAD
                     AGEAIEAAEAMLVGGRFGEAGRRIVVEELLRGREASFFVLADGRGFLELATCQDYKRA
                     GDGDTGPNTGGMGAYSPSVHLDAEMRAQIHREIVEPTIAGLRAEERDFRGVLYVGLML
                     TGDGPKVLEYNARFGDPETQVMMPRLDGDWLELLHAAATGRLNGREIAWKTDPAVCVV
                     MAAEGYPGSYAKGWPIDGIAEARAVGGVVVFHAGTARDAARRLVTAGGRVLGVTATAG
                     DLGQARRRAYLAVEAIDWQGRHYRGDIARDAIAARTEPRREGSDD"
     gene            complement(8490..8801)
                     /locus_tag="VD788_11610"
     CDS             complement(8490..8801)
                     /locus_tag="VD788_11610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXV76951.1"
                     /translation="MPIYEYRCIKCGHHIEIIQKLSDRPLRKCPECGGRLEKLVSRAA
                     FLLKGGGWYAEGYSKSADQKAEAEPAASPAKAETKGAASPDESKSKSKSNKSKKNASS
                     T"
     gene            complement(8900..10663)
                     /locus_tag="VD788_11615"
     CDS             complement(8900..10663)
                     /locus_tag="VD788_11615"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXV76952.1"
                     /translation="MTARRRLARRWLFGLLSCCAAISSVLDTGGAGFTAVCAQDEAPR
                     PDTLSLSSDLRVRVLRGRDVELEVRAAYGDDWRTIAERVSAAAGATAALAAWNGGEVT
                     PERWLRVPLSLLNDDYRALVLVSLFPEDRRDGSDWVHLARSGRLPTYDAGLWQVAEWF
                     AGDGDAFGELLAANRLGSPELRQGQAVRIPGSLLHPAFRGGDPATDTELEFERDEHGP
                     YAAYRLKRGEALYSAVVVRYTGRTKASDVVTAAELIKERSGIRDLRDIPVGFKIKIPF
                     ELLEPQFLPPTDPRRIASEAERREVEEAMARIPRGDGHGLDGVVIILDPGHGGRDLGT
                     MNNGIWEHDYVYDVTCRLKRRLEQYTVGRVYLTLVDEETGCAPSEADRLHANRQGTVQ
                     TDPPFLARENGEAKIGVNLRWYLANSIYRREVAAGVDPGKILFLSLHADARHPSLGGV
                     MVYVPGAANVGETHGFGSKTYTRYAEVREQQWIKLTKKERIRSEAASLRLAEGIVDAF
                     RALDLPVQPYQPVRNRIIRGRSTFVPAVLRGNVVPAKVLVEMVNLSNGDDAALLARAA
                     DRERLAEALHRALVEHFGADR"
     gene            complement(10713..11552)
                     /locus_tag="VD788_11620"
     CDS             complement(10713..11552)
                     /locus_tag="VD788_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76953.1"
                     /translation="MSRTAPSTAALICVAGSLVAWPGSAGAAPPAIERRTIVVVEVRP
                     LTAERVGERADRPLRLVLDSEDDAGALVELGWPTAADRSRMTFRVAHAPAVAGSERAV
                     ALDARLVLPDGTSVHASRTIAFDENETTLFEIHRFDDRSLTLAVELATESELVVSSRL
                     TPGSPIRFRLEIVRVLDGRSIPLENDYLNTLIGEEVSYAFRLADTADADSVSITIAPR
                     RLVGDIAEIDISVSGKLPVGDELMIIGRTERWLASRDATSTLAFEAGQPPTGYRFLLT
                     ARF"
     gene            11655..13736
                     /locus_tag="VD788_11625"
     CDS             11655..13736
                     /locus_tag="VD788_11625"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXV76954.1"
                     /translation="MNPSAKLDWVATPSRLPDRSRWLAALVSLSIFPLVAGPEVLGQD
                     APRPFAGFVSDAVGNVLQNVELLIVDTRRPAEPLASARSDERGRFRFAGVPRGVYRLA
                     ALKEGYLTFIARVDTDVENWHEIVLHPVSAADPDRREAVPDDSSWVYRLPRRYVLHDR
                     EPEPPRESLRAAPPASRPERREGWSVRLDRLVAVPADTTVDGTGGPEMRGSETGLEIS
                     STVGARGRMAVRGFQREFASTRVDAAGPSAVTRDARSMQLSFAYETGPAAELAVSAFY
                     AEREVAYAATPPETGAAIGLAGSTSDGHTHWGYDASWATRLDESSSLRLSVDYRDTTL
                     NGVGVAGVGGIAADGAADGVSARTVGASGVYAHAPVDDHELRFDVRAQRLDTRNVAAA
                     AGEPTVLTPVDFALAGWSVGLDAQDTWSVGPPLSLVYGLGYRQATGTRRAALFVPRVG
                     AGWRFHGVRLDAMVSYHEVTDWTPRDAGRWSAPFRPAETWGYQAELRVPVGSGVSVTG
                     ATAFSPLQLDPLGYAGAGFETDTRPIYLTDGNTAAREDRLAIAQQSQRTRTYVEYARG
                     SAAGQLAPITFDAVQTDFLIERTLDYGAGRFGLEVVPSGTGVVLQYQTLLCRSTSSGD
                     GLDTREESIELRVTQQLADGLPVGDWRLLFAVRRSWLTSFGETDVAVARDPELLDTLN
                     HRLTAGLSVSF"
     gene            13962..>14234
                     /locus_tag="VD788_11630"
     CDS             13962..>14234
                     /locus_tag="VD788_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXV76955.1"
                     /translation="MARRSRKVGVWLVLILAGPLLVSGLVAFARRVNAAAPEVGVEWT
                     QSSLGAVAIEVDESSPAARVGLRPGDRLITVDGRPIASSLDAGELAW"
BASE COUNT         2071 a         4996 c         4965 g         2202 t
ORIGIN      
        1 atctcgcggc gcgcgaacat cttcacctga ccgctgtgga cctgccgcat gagcgagctg
       61 taggcgccga ggaggagctg ctgcccggtc tggccgcggg cgtagaacgt gcgcgccacc
      121 tgcgcgccgc cgaaggagcg attggcgagc atgccgccat actcgcgggc gaatggcaca
      181 ccttgcgcga cgcactgatc aatgatgttc acgctgacct gagccaggcg atagacgttg
      241 gcctcgcgcg aacggaaatc accgcccttg atcgtgtcga agaacaggcg atagacgcta
      301 tcgccgtcgt tttggtagtt cttggcggcg ttgatcccgc cctgcgccgc gatgctgtgc
      361 gcgcgccgcg ggctgtcgtg gtagcacaag cagcggacac ggtagcccag ctcggccatg
      421 gtcgccgcgg cggaggcacc ggccagcccc gcgccgacga cgatcacgcc gtgccggcgc
      481 ttgttcgccg ggttgaccag cttcagctcg aagcggtgcc gatcccactt cttctcgatc
      541 ggtccgtcgg gaatgttggc ggcgatttcc acctgtgagc cccctggtct aacgcaccac
      601 gccgatcagc acggcgacgg ggatcgagac gttgccgatc acgatcgcga gcgcgagcgc
      661 agcggccagc ggacggcgcc aggtcttgac ccgctcgccc tcgagcgcca gcgtttgggt
      721 cgtgctccac atgccgtggt agaggtgaag cccgagcggg atcatcgcgg cgacgtaagc
      781 cgccgaaacg ggccattgct tgaagcccgc aacgacgttg cggtaggccg atccggcgac
      841 gaagtccgga tgcgcggtgc cgaaggtcag gtgcagcagg tgatagacca cgaacgccac
      901 cagcagcacg ccaccccagc gcatcgtccg cgacgcgcgg ctgaacgaca ggtcgtcgaa
      961 cttgcggtag cgcgcggagc gcgcgcgacg gcagcgcagg tacaggtctg tcgccgcgta
     1021 gacgtgaacg ccgacgcaga acagcagcac gaaccgtgcg atccacagca cctgcccgtg
     1081 gccgaacacc ggcgcgccga cggtgcggag gaactcagcg tagcgatcgt acttctccgg
     1141 accctggtag atcttcagat tgcccagcat gtggacgaag acgaatccgg cgagcacgat
     1201 ccccgttgcg gccatcagga ctttcttgcc gacggaggac tgggtgagat gtagcacgtc
     1261 gcgcatcgtc aactcgagcg atcgaacggc aagggagcag gatcataatg attccgccac
     1321 gcgtccgcaa ccgcgtgcag cgcttggccg gtgcgccggc gtgcgataat cgtcgggtga
     1381 atcccggccg tttcactccc gatcgttcgg acgactgacg cgatgtccga cgggagcggc
     1441 acgcgcttcg tcgaccggct gctcgaccat ccttcgctcg cgccgatggt cgtgcaccac
     1501 cgccgcatcg agcgggtgcc ggccgtcctc gcccaaccgc gcgatccact gccgcccgcg
     1561 atcgaggccg cgctgacgac ggctggactg ggcggtctgt accgccacca ggccgagggc
     1621 atcgaccgct tgcgggagag gcgcgacgtg ctgctggtca cgccgaccgc gtccggcaag
     1681 accttgctgt tctcggtcgc ggtgctcgaa acggcgctcg ccgatccgtc gagccgtgcg
     1741 ctgttgctct acccgaccaa agcgctggcg caggaccaac tcgccgggtt ccgcgcgctg
     1801 gccggcggtg tcggggcgct gcgcccgccg agcttcgaga tctacgacgg tgacacgccg
     1861 caagcccgcc gtcgccacat ccgcagcgat ccgccgtcgg cactgatcac caaccccgac
     1921 atgctgcaca tgggcatcct ggcccatcac cacgattggg ccgctttcct gcgcggcctg
     1981 cgctgggtcg tgctggacga gctgcatgtc taccgcggca tgttcggtgc tcacgtgcac
     2041 cacatcctgg cccgtctcga gcgactgtgc gcccattacg gcgcgcgacc gcagttcatc
     2101 gccgcctcgg ccacggtcgg caacccggcc gcgttcgccc gcacgctcgt cgggcgggag
     2161 ttcgtcgtga tcgatcgttc cggcgcaccc gcctcggcac gcgacgtcgt cttcgtcaac
     2221 ccgatcggtg tctcgccgta caccgtcgcg gtgcgcgtgg tggccgaggc gattcgcgac
     2281 cggcggcgta cgatcgcgtt cgccaaggcc cggcgggtca ccgagctgct gcacagctgg
     2341 ctggtgcaac acgagccgga gttccgctcg cgcgtcgcac cctaccgcgc gggctacctg
     2401 ccggaggagc gccgcgcgat cgaaggtcgc ctgttccgcg gcgagctgtc ggcgatcctg
     2461 tcgacgagcg cgctcgaact ggggatcgac gtcggcgcgc tcgacgtctg cgtgttggtc
     2521 ggctatcccg gatcgttgat ctcctcctgg cagcggatcg ggcgcgtcgg ccggcgtcgc
     2581 gacggcggcg cggtggtgct gatcgcgatg ccggacgccc tcgaccagta tgtcgtgcgt
     2641 catcccgacc tgttcttcgg cggccgcttc gaaaacgcgg tgctcgaccc gtggaaccgc
     2701 cagatcgcgg gtcgccacct cgtctgcgcc gccgccgaac ggccactcgc ggccggtgag
     2761 tacgaccgcg cgggacagag cggccggcag ctgatcgatg aactggtgcg cgacggccgg
     2821 ctggtgcagg acgccgccgg gacacggttc ttctcgttca agcgacgacc gcaacgcgag
     2881 gtgagcgtgc gcgccgcggg cgagccgttc gtgatcgtcg acacggcggg caagctgctc
     2941 ggtacgatcg atggccaacg ggtctatcac gagtgtcatg ccggagcgat ctaccttcac
     3001 ggcggtcgtt cgtttctcgt tcgcgccctc gacgccgatc ggcgtcgcgt gacggccgac
     3061 cgggccaacg tcgactacta caccgtcgtg ctgggcgaca aggaaaccgc gatcctcgag
     3121 cgtctcgacg cgcgccgcct cggcgacttc cccgtcgggc tcggtcggct cgaggtcacg
     3181 gtgcgcatcc gcggctacca gaaaaagcgt ctgttcgatg gcgagccgat cgcccagcat
     3241 ccgctcgacg tcccaccgct ggtcttcgag acggtcggat tgtggatcga gctgccggcc
     3301 gagctgccgg ccgcctacgc ggcgcgggag ctgcacttca tgggcggaat ccacgccgtc
     3361 gagcacgcga tgatcgggct gtttccgttg ctcgcgatcg ccgaccgcga cgacgtcggc
     3421 gggatctcct acaccggcca cccgcagctc ggcgctccgg ccgttttcgt ctacgacggg
     3481 ttggcggggg gcgcggggct ggccgagcag gggtttcgcg agatcgagca cctcgtcgga
     3541 cgcacgctcg agctggtgcg gagctgcgac tgcgaggacg gttgcccggc ctgcatccag
     3601 tcgccgaagt gcggcaacgg caacaagccg ctcgacaagg ccgccgcgct gctgacgctc
     3661 cgcttgctcg gcggcgagga ggcgctcgcc tcgctcgggg tcgaggccgc ggccgagggc
     3721 gccgagcgag cgatcccgcc cgccccgcgc ctcgacccgg cgccgatcga gtccctcgcc
     3781 accggcggta ccgcgcggcg ccacggcatt acgcgctcgg gtaacctcga gccggcgcgg
     3841 gcgctcgacg tgcgcccgtc gccgccgatc gcccagcgaa cgctgatctt cgatctcgag
     3901 acccagcgcg gcgcggacga agtcggcggc tgggcgcacg tcgagcggat gggtctggcg
     3961 ctggccgtgg tctacgacac ggcacgcgag gtcttccgca cctacttcga gacggacgtc
     4021 gaccgcctgc tgctcgacct ggtgatggcc gaccgcgtgg tggggttcaa cgtggaccgc
     4081 ttcgacatgc gcgtgctggc cggctacacc gactgggatc tctcgcgggt gaggacggtc
     4141 gacatgctcg ggctgatcca ccggcggctc ggtttccgcg tctcgctcga gcacctgtgc
     4201 gaagccaacc tcggcgagag caaggtcggc gacgggttgc agagcctgcg ctggtggaag
     4261 gacggccgga tcgacctgat cgagcagtac tgcaaacgcg acgtcgagct gacacgacgg
     4321 ttgtgggacc tgggacagcg gcggggttac ctgctctatc gcgaccacga gcagcggacc
     4381 gtacgggtgc ccgtcgagtg gtgagaccgc cgggcgggac gtggaccgcg ccggcgggta
     4441 gaatgccgca tcacgcgtgt cgaggaaacg gaatgggacg accgtgggcc gccgtttcgg
     4501 ggttggtgct cgccggcctg ttcggtgctt gtggcgagcc gtcgacgggg atgatggtct
     4561 cgatccctcc gcccgacggc tggcgcgagc agctcgaggg ttcccgatcc gacaaggacg
     4621 agttcttcaa gaccgacccc ggctcgccgc tcctgccggc ggagacggcg gcgttcgacg
     4681 ggctcgacta ctgggagcct aacgccgact actaccacgt cgggcgcgtc aatctctacc
     4741 tcgagcccca gaagttcgac gtcgtgacga cgtccggcgt catccgtccg tgcgccaagg
     4801 tcggctgggt cgggttccgc cagggcgggc aggatcacaa gctgcaggtc taccgcttgc
     4861 tcgaccaggc gccgcagcag ggtggggccg actacttcct gccgttcatg gacgaaacga
     4921 ccggcgagga gacctacccc gcggggcgct acgtcgatct ggtcggcccc gagggcgggc
     4981 cctacgtgct cgacttcaac acggcgagca atccgtggtg cgcctacggt gacgtcgacc
     5041 gcttcgtctg tccggtgacg cccccggaaa accgtctccc cgtacggatc gaagccggcg
     5101 agcgcggcta tcgccaatcg ccccgggcgg aagacggggc cggatgactc gccaggtggc
     5161 gcccctcgac gcgggccggg ccgatggccg ggcggtcgtc gcacgggcgc gtgcgagcgc
     5221 gaccgcgctc gtcgcgacgg tgctggcagc gacggcggcc agctcgctgc ttccgtccgt
     5281 gcgctcgctc gacgtgctgg tcggtccggc cgccgcgatc ggcgcgatcg gacccgcgat
     5341 cggctaccgg cgctatctgc tggcgcggga tcggcggggc agctacctcc gcgccgtcgt
     5401 ttccgcgctg gccgtgaccg gggtcgccgc cctgttcggt gcggcggtgc acggcgtgac
     5461 cggcgagttg ctgccgttga tcggcccctt catgcatgtc ctgctggccg ccgtcatctg
     5521 gccggccgac gagcgcctct cggcgttcag cggtgcgccg agcggccccg ggaagagtta
     5581 gtccggtggc gccgccggtc gggtcccgca ccgtcttcct cgtcgggttc atgtgctgcg
     5641 gcaagacgac ggtaggccgt cggctggcag agcgccgcgg cgggtccttc atcgacacgg
     5701 acgagcggat cgcccgacac accgggcgga cgatcgaggc gatcctcgcc ggcccgggcg
     5761 aggaggagct ccggcggctc gagatcgact gcctgcgatc gctcccgcag gccggagcga
     5821 ctgtcgtcgg caccgggggc ggcgcctatt cgcgttacga agttcgccgt tcgctcgcct
     5881 gccgcggtct gacggtctgg ctcgacgtct ccttgggcga ggcgcggggg aggcggaagg
     5941 cggacgccac gcccagaccg ctgtggtccg actcggacga cgcggtcgcc ttccgcgcgt
     6001 tcttcgaacg gcgtcgcgcg gtgtacgcga tggccgaggc gcgagtcgat gccgaccgcg
     6061 ccccggacga ggtcgtccgc gcggtcgagc gtctgctcga cgagccggaa tctcactttt
     6121 ttcattgatt cttgcggcgg aatggtgata cctatgccgg acgacacggc cgtcgagccg
     6181 cgaagggatc ttcccgctgg aggcttacgc atgaccagaa ccgagctgat cgacgatctc
     6241 gccaaccgta cgggtaccga gaaaaaggac gtcaagtcct tcctcgagca cctgacgtct
     6301 ttgatcgaag acgagatcaa gaagggcggt gaggtgccgc tgaaagggct ggggaagttc
     6361 cgtgtccaga accggaaggc gcgtgtcgga cgcaacccgc tgaccggtgc cgagatacaa
     6421 attcccgcca agaccgtggt gaagttctcg gtcgccaagt cgctcaaaga tctggcctga
     6481 ccggcgagcc ggccccggtc cgctcggtcg ccggggctcc ccgcacccat ccgtcccaac
     6541 ccaggcttcc ggcccagccc ggggcccgtc gccgggtcag tcaccgacga gcgaccgcaa
     6601 cttgcccttc gcgcgttcgc tcttggcctc gatcgcatcg gccatccgcg cccgctcggt
     6661 cgccagcttc gcggccagcc ccgggtcggc cgtcgccagg atcgaagcgg cgagccatcc
     6721 cgcgttcgcg gcacccgcct tgccgatcgc cagcgtgccc accgggacgc ccgccggcat
     6781 ctggacggtc gccagcaacg cgtcgagccc gttgagatcg ctcgacgcga gtgggactcc
     6841 cagaaccgga cgcgtactgt gggccgcgac cacccccgcc agatgtgcgg cggccccggc
     6901 accgacgatg aagaccgcga cgccgcggtc cggggcggtc gcgacgatcc gcgtcgtgcg
     6961 ttccggcgac cgatgggccg aggtgacgtg catctcgtag ggcacctcga gctgctccaa
     7021 gaccttgatc gcctcggcca tcaccgggag atcgctgtcg ctgcccatca ggaccgaaac
     7081 acgcgcccgc gtccgttcag tcatcggaac cctcccttcg aggttcggtc cgggcggcga
     7141 tcgcgtcgcg tgcgatgtcg ccgcggtagt gccggccctg ccagtcgatc gcttccacgg
     7201 cgaggtaggc ccgccggcgg gcttggccca ggtcgcccgc ggtcgcggtc acgccgagga
     7261 cccgcccgcc cgcggtcacc agtcgtcgcg ccgcatcgcg cgccgttccc gcgtggaaca
     7321 cgacgacacc gccgacggcc cgggcctcgg cgatgccgtc gatcggccac cctttggcgt
     7381 aggatccggg gtagccctcg gccgccatca cgacgcagac ggcggggtcg gtcttccagg
     7441 cgatctcgcg cccgttcagc cgacccgtcg cggcggcgtg cagcagctcg agccagtcgc
     7501 cgtcgaggcg gggcatcatc acctgggttt ccgggtcgcc gaaccgcgcg ttgtactcga
     7561 gcaccttcgg gccgtcgccg gtcagcatca atccgacgta cagcacgccg cggaagtccc
     7621 gctcctcggc acgcaggccg gcgatcgtcg gttcgacgat ttcccggtgg atctgcgcgc
     7681 gcatctcggc gtcgaggtgg accgacggcg agtaggcgcc catgccgccc gtgttcggcc
     7741 cggtgtcgcc gtccccggcg cgcttgtagt cttggcaggt cgcgagctcg agaaagccgc
     7801 gtccgtcggc caacacgaaa aacgaggcct ctcgtccccg cagcagctcc tcgacgacga
     7861 ttcgacggcc ggcctcgccg aatcgtcccc cgaccaacat cgcctcggcc gcctcgatcg
     7921 cctcgcccgc gtccgcacag atcgagacgc ccttgccggc cgcgaggccg tcggccttga
     7981 ccacgagcgg gtagggccgc ccccgcgcac cgagatagtc ccgagccgcg gcggcgccgt
     8041 cgaagacgcg gtagtccgca gtcgggatgc ggtggcgcgt catgaaccgt ttcgcgaaca
     8101 ccttgctcgc ctcgagctcc gccgcccgcc gcgtcggtcc gaagacggcg acacccgcct
     8161 cgccgagtcg atcggccaag ccgccgacga gcggagcctc cggaccgacg acgaccaggt
     8221 cgtacgcccc gctcgtcgcg tgctcgacca gcgcggacac gtcgtcggcc gcgatggcga
     8281 ccgttcgatg gtcgtcggcg atgcccgcgt tgccgggagc gacgacgagc tcgtcgagtt
     8341 gcggactgcg tccgagcgcc caggccagcg cgtgctcgcg cccaccaccg ccgacgagca
     8401 agactcgcat cgtgtcgtcc ctaggagggg aaagcgggaa ttatcccacg gcaccatccc
     8461 gccggcaacg gtgggcggcc ggagcgcgct caggtgctcg atgcgttctt cttgctcttg
     8521 ttgctcttgc tcttgctctt gctttcgtcg ggcgacgcgg cgcccttggt ctccgccttc
     8581 gccggggagg cggccggttc ggcctcggcc ttctgatcgg ccgatttcga gtaaccctcg
     8641 gcgtaccagc caccgccctt gagcaggaag gcggcgcgcg agacgagctt ctcgaggcgc
     8701 ccgccgcact cggggcactt gcgcagcgga cggtcgctga gcttctggat gatctcgatg
     8761 tggtggccgc acttgatgca gcggtactcg tagatcggca tcgactccct cggccggggg
     8821 cgacgggcgc cgccgtaatc ggaaaaccta gcagcgggag gcccggtcgg caatcggccg
     8881 tccgcggcgc cgtcccggct cagcgatcgg cgccgaagtg ctcgaccaat gcgcgatgga
     8941 gcgcctcggc cagtcgttcg cggtcggcgg cccgggccaa cagcgcggcg tcgtcgccgt
     9001 tgctcagatt caccatttcg acgagcacct tggccgggac gacgttgcca cgcagcaccg
     9061 ccgggacgaa ggtcgagcgc ccgcggatga tgcggttgcg caccggttgg tagggttgga
     9121 ccggcaggtc gagcgcccgg aaggcgtcga cgatcccctc ggccagccgg agcgacgccg
     9181 cctcggatcg gatccgctcc ttcttggtca gtttgatcca ctgttgctcg cgcacctcgg
     9241 cgtagcgcgt gtaggtcttc gagccgaaac cgtgggtctc gccgacgttg gccgcgccgg
     9301 ggacgtagac catgacgccg ccgagcgaag ggtgccgcgc gtcggcatgc agcgacagga
     9361 acaggatctt gccggggtcg acgccggccg cgacctcgcg gcggtagatc gagttggcca
     9421 ggtaccagcg caggttgacg ccgattttcg cctcgccgtt ctcgcgggcg agaaacggcg
     9481 ggtccgtttg caccgttccc tgtcggttcg cgtgcagcct gtcggcctcg gacggcgcgc
     9541 aaccggtctc ctcgtcgacc agtgtcaggt agaccctgcc cacggtgtac tgctccaggc
     9601 ggcgcttcag ccggcaggtc acgtcgtaga cgtagtcgtg ctcccaaatg ccgttgttca
     9661 tcgtgccgag gtcgcggccg ccgtgacccg ggtcgaggat gatgaccacc ccgtccagac
     9721 cgtgcccgtc cccacgggga atgcgggcca tcgcctcctc gacctcgcga cgctcggcct
     9781 cggaggcgat acgacgcggg tcggtcggcg gaaggaactg cggctcgagc agctcgaacg
     9841 ggatcttgat cttgaacccg accgggatgt cgcgcaggtc gcgaattccg ctccgctcct
     9901 tgatcagctc cgccgccgtg acgacgtccg aagccttcgt ccgtccggtg tagcgtacga
     9961 cgacggcgga gtagagcgcc tcgccgcgtt tgagccgata ggcggcgtaa ggcccgtgct
    10021 cgtcgcgctc gaactcgagc tcggtgtcgg tcgcgggatc gccgcctcga aacgcggggt
    10081 gcaacaggga ccccgggatc cgcaccgctt gcccctggcg cagttcgggt gagccgaggc
    10141 ggttggcggc gagcaactcg ccgaacgcgt cgccgtcccc ggcgaaccac tcggcgacct
    10201 gccacaggcc ggcgtcatag gtcggcaggc ggccggatct cgccagatgc acccagtcgg
    10261 atccgtctcg acgatcctcg gggaacagcg aaacgagcac cagcgcgcgg tagtcgtcgt
    10321 tcaacagcga caacggaacg cgtagccagc gctccggcgt cacctcgccc ccgttccacg
    10381 ccgcgagcgc ggcggtcgcg ccggcggcgg cgctcacacg ctcggcgatc gtccgccagt
    10441 cgtcgccgta cgcggcgcgc acctcgagct cgacatcccg gccgcgaagc acgcgaactc
    10501 tgaggtccga gctgagcgac agggtgtccg gtcgtggcgc ctcgtcctgg gcacagacgg
    10561 cggtaaagcc ggccccgccg gtgtcgagca cggacgagat cgcggcgcag cacgacagca
    10621 acccgaacaa ccagcgccgg gcgagccggc gtcgagcggt caccgcggtc gttccgtctg
    10681 gagcagagca tcgcacgggt cggagaccgg tcctagaacc gcgccgtcag caggaaacgg
    10741 taacccgtcg gcggctggcc cgcctcgaac gcgagggtcg atgtcgcatc ccgcgatgcg
    10801 agccagcgct ccgttcgacc gatgatcatc agctcgtctc cgaccggcag tttgccactg
    10861 accgagatat cgatctcggc gatgtcgccg accaaccggc gcggagcgat cgtgatcgag
    10921 acggaatccg cgtcggccgt gtcggccagg cggaacgcat acgacacctc ttcgccgatc
    10981 agcgtgttga ggtagtcatt ctcgagcggg atcgaccggc cgtcgagcac gcgcacgatt
    11041 tcgagccgaa accggatcgg cgagccgggg gtcagccgcg acgacaccac cagctcggac
    11101 tcggtcgcga gctccaccgc caacgtcaac gagcggtcgt cgaaccgatg gatctcgaac
    11161 agcgtcgtct cgttctcgtc gaaggcgatg gtgcgcgacg cgtggacgga ggtgccgtcc
    11221 ggcaggacca gccgggcgtc gagcgcgacg gcccgctcgg atcccgcgac ggccggggcg
    11281 tgcgcgacgc ggaacgtcat ccgcgaccga tccgccgcgg tcggccaccc gagctcgacc
    11341 agggcccccg cgtcatcctc cgagtcgagg acgagccgca gcgggcggtc tgcgcgttca
    11401 ccgacccgct cggcggtcag tggccggacc tcgacgacga cgatcgtccg acgttcgatt
    11461 gcaggcggtg ccgcgccggc cgacccgggc caggcgacga gcgacccggc gacgcagatc
    11521 agggcggccg tcgacggggc tgttcgcgac acgggacctc cctccgcctc gcgcgaggcg
    11581 gcctcgccca tcttattgga aaaccgacta ttgacaaggg aaaccgacgg caatacaatc
    11641 cgcgcgctac ctatatgaac ccaagcgcca agcttgactg ggttgcgaca ccgagccggc
    11701 tcccagaccg atcgagatgg cttgccgcgc tcgtttcgct ttcgatcttc cccctcgtcg
    11761 ccggcccaga ggttctcggg caggatgctc cgcggccctt cgccgggttc gtctcggacg
    11821 cggtcggcaa tgtcctgcag aacgtcgagc tgctgatcgt cgacacgcgc cggcccgccg
    11881 agccgctggc cagcgcgcgg agcgacgaac gcgggcggtt ccgcttcgcc ggcgtgcccc
    11941 gcggcgtcta ccggctggcc gcactgaagg agggatacct caccttcatc gcgcgggtcg
    12001 ataccgacgt cgagaattgg cacgagatcg tgttgcatcc ggtctcggcc gccgaccccg
    12061 atcgccgcga ggccgttccg gacgattcgt cctgggtcta ccggttgccg cgccgctacg
    12121 tcctgcacga ccgcgagccc gagccgccga gggagtcgct ccgagcagcg cccccggcct
    12181 cgcggcccga gcgccgggag ggatggagcg tgcgcctcga tcggctcgtc gcggttccgg
    12241 cggacaccac ggtcgacgga acgggcggac ccgaaatgcg cggcagcgag acgggcctcg
    12301 agatcagctc gaccgtcggc gcccgcggta ggatggccgt tcgcggcttc cagcgcgagt
    12361 tcgcctcgac ccgggtcgat gccgccgggc cgtcggccgt cactcgtgac gcgcgatcga
    12421 tgcaactgtc gttcgcctac gagacgggcc cggcggccga gctggcggtg agcgcgttct
    12481 acgccgaacg agaggtcgcc tacgcggcga cgccgcccga gaccggcgcg gcgatcgggc
    12541 tggccggctc gacctccgac ggtcacaccc actggggtta cgacgcgagc tgggcgaccc
    12601 ggctcgacga gtcgagctcg ctcaggctga gcgtcgacta ccgtgacacc acgctgaacg
    12661 gggtcggggt cgcgggagtc ggcggcatcg cggccgacgg tgcggccgac ggcgtgtcgg
    12721 cccgcacggt cggagcgtcc ggggtctacg cgcacgctcc ggtcgacgat cacgagttgc
    12781 gcttcgacgt ccgggcgcag cgcctggata cccgcaacgt ggcggcggcg gcaggggagc
    12841 cgaccgtact cacgccggtc gacttcgcgc tggccggctg gagcgtcggg ctcgacgcgc
    12901 aggacacctg gtcggtcggt ccaccgctgt cgttggtcta cgggctcggt tatcgccaag
    12961 cgaccggcac gcgccgggcc gcgttgttcg tgccccgcgt cggcgcgggc tggcgcttcc
    13021 acggcgtgcg actcgacgcc atggtgagct atcacgaggt caccgactgg accccgcgcg
    13081 acgccgggcg gtggtccgcc ccgtttcggc cggccgagac gtggggctac caggcggagc
    13141 tccgcgttcc cgtcggctcg ggcgtcagcg tcacgggcgc gacggcgttc tcgccgctcc
    13201 agctcgaccc gctgggttac gccggggccg gcttcgaaac cgacacccgg ccgatctatc
    13261 tgacggacgg caatacggcc gcgcgcgagg atcggctggc gatcgcgcag cagtcgcagc
    13321 gcacgcgaac ctacgtcgag tacgcccgcg gatcggccgc gggacagctc gccccgatca
    13381 cgttcgatgc ggtgcagacc gacttcctga tcgagcgaac actcgactac ggcgccggcc
    13441 ggttcggtct cgaggtggtg ccctccggga cgggcgtcgt gctgcagtac caaacgctgc
    13501 tgtgccggtc gacttcgagt ggggacggcc tcgacacgcg cgaggagtcg atcgagttga
    13561 gggtgacgca gcagctggcc gatgggctcc cggtcggcga ctggcgactg cttttcgccg
    13621 tacgtcgctc ctggctcacc tcgttcggcg agaccgacgt cgcggtcgcg cgcgacccgg
    13681 agctgctcga cacgctcaac catcgactga cggccggtct gtccgtttcg ttctgagcta
    13741 tttctttggc ctccgcgaag aatcgatcca ttttctcgta gaatgttccc gcatccgtcc
    13801 aaacgattgg acgcggctcg cggttgcgtc cgccttcggg tccgggtcgc gactcgaaac
    13861 ggccgaaacc cggcgcatcg aggacgcttc gacggccgcc ggcatggcgc gtcgcttgct
    13921 tgtcgtccgg cggggcggtc gtgcagccgc ccgaggtggg agtggcacgt cgtagccgaa
    13981 aggtaggggt ctggctggtg ctgatcctgg ccgggccctt gctggtctcc ggtctggtcg
    14041 ccttcgcacg acgcgtcaac gccgccgcac ccgaagtcgg ggtcgagtgg acccagtcat
    14101 cgctcggcgc cgtcgcgatc gaggtcgacg agtcgtcgcc ggccgcccgg gtgggattgc
    14161 ggcccggcga ccggttgatc acggtcgacg gacggccgat cgcgagctcc ctcgacgccg
    14221 gcgagttggc gtgg
//
LOCUS       DASPGN010000401        11303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_189654, whole genome shotgun sequence.
ACCESSION   DASPGN010000401 DASPGN010000000
VERSION     DASPGN010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189654"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VD788_11635"
     CDS             <1..275
                     /locus_tag="VD788_11635"
                     /inference="COORDINATES: protein motif:HMM:NF020839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MerR family DNA-binding protein"
                     /protein_id="HXV76956.1"
                     /translation="ELGRLPDDRVPEGADWGRLSARWTRRIDERIALLERLRTTLTGC
                     IGCGCLSLDRCRLANPGDRLARLGPGPRYWVDGGRGRAPVGDDEPG"
     gene            complement(293..1522)
                     /locus_tag="VD788_11640"
     CDS             complement(293..1522)
                     /locus_tag="VD788_11640"
                     /inference="COORDINATES: protein motif:HMM:NF014510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HXV76957.1"
                     /translation="MIETSLDFLSSLRDVVRVADLIDILIIAACVYFAILWLLRRASR
                     GVVVGLSLVAFVYLLARQLQMALTSWLFQVGFTAILIGLILVFQKDIRSVFERLGALV
                     GIVGRSAGPASGTVEVLARSLAALARRKIGAIVVLPGRASLDPHVYGGYALDGRVSEP
                     LIASIFDPSSHGHDGAMIVSGDRVASFGVHLPLSERLDQLAGRGTRHAAALGLSELTD
                     ALVFVVSEERGTLSVAQRGRMEAVVDPAALETRIGSFHRETGAADEHSAGPFAGAVDW
                     LRPALLHHWPTKLLSIALASMLWLTVAYRVEPVVRQLSVPVEFHDLPETLQITDVNPE
                     VITLTLSGPKHRFELLDPDELRVSLDVSDTEPGRSVRPFRQEHVDIPSEYFTISDFSP
                     DSVSFTIEPRPPAPPDS"
     gene            1697..2782
                     /locus_tag="VD788_11645"
     CDS             1697..2782
                     /locus_tag="VD788_11645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV76958.1"
                     /translation="MSVRSIVGLGAILVVVAGLAVPRLIDGRAGGSRPAPSAAAPTRL
                     AVEVLEVEPRRMAERLATTGTVRANESVDLVSEIAGVVRTIHFDEGATVEQGELLVEI
                     DDTELQAQRDRVLYRLRLAENREARQRELREQGVVSEQDYDVADNELNVLRADLRLIE
                     AELAKAAIRAPFSGVVGLRYVSTGSLVSRDTLIATLQDLDPAKIDFTLPEKYAGRVGA
                     GDPVEFRVRGSDRVHTARIYAVEPRIVAETRSLLLRATTANDERALLPGAFADVTLAV
                     DEVADAIVVPSLAVVPELGGKKVFVVENGRAEARRVETGIRTDTEIQITAGLRPGERV
                     IVSAIQRLEPGLPVDELPGATGAAAAR"
     gene            2779..5865
                     /locus_tag="VD788_11650"
     CDS             2779..5865
                     /locus_tag="VD788_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV76959.1"
                     /translation="MSLYSLSIRRPVLAIVMSIAILIFGLLGLSFLGVREFPAVDPPL
                     ITVSTDYRGANADVIETQITEPLEESINAVAGIRTMTSTSREGRSTIIVEFGLESDLE
                     RAANDVRDAVSRALGNLPPDVEPPQVSKADADASPIVQVTLGSDTRDSLELTRLADEL
                     FAERLQTIPEVARVDIWGDKTYAMRLWVDPQKLAAYRLTPLDLRRAVERENVELPAGR
                     IEGGTIELPVRVLSRLSTPDEFNAMILKEDAGGVVRLRDVGQAVLGPRNERTVLKKDG
                     VPMVAVVLRPQPGANYIEIADEFYRRVEQIRRELPADVTVEYGFDTTDFIRKSIAEVG
                     QTIVLALLLVVSIIFLFLRDLRTTVIPLLVIPVSLVGAFFVMYALGFSVNVLTLLALV
                     LAIGLVVDDAIIVLENIYAKIEAGLAPLQAGVAGTREIFFAVVATTLALVAVLLPVMF
                     LGGLTGRLFREFGATLAGAVIISSFVALSMTPMLCTRILRRYEGHSRFHRATEPFFRS
                     LAAAYRDTLERLMTRRWLAVPLIAGCGGLITLFFWLLPAELAPIEDRSGLRLSVRGPE
                     GATYPYMGRVMDDLGAAVAEAVPEAEQIIWVTSPGFGASSSVNSGFLRLRLVPPEQRE
                     RSQMEIADALGAVTRRLTGAQIFVTQEPTIAVGRRRGLPVQFVLQAPTLERLKDVLPR
                     FLDEARGDPAFSEVDVDLLFDKPELTVDIDRPRARDLGISVLDVAQTVQLALSEQRLG
                     FFVMDGKQYEVIGQVTSDNRNETIDLRNLYVASRSGAPVLLDKLVTIAERSSPPQLYR
                     FDRYVSATVSAGLAPGLTISQGIDAMQAIADRLLDDTFHTTLAGESRDYQESSNTLVF
                     IFGLAIVLLYLVLAAQFESFRDPFTIMLTVPLALAGALFALWWYGLTLNVFSQIGMIM
                     LIGLVTKNGILIVEFANQRRAAGRPLIEATVEAAAARLRPVLMTSLSTVLGILPIALS
                     LGAGSASRVPMGVAVIGGLTFGTLLTLFVVPAMYTFLTARTLVRVGADDLEGHEAA"
     gene            complement(5877..6815)
                     /locus_tag="VD788_11655"
     CDS             complement(5877..6815)
                     /locus_tag="VD788_11655"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HXV76960.1"
                     /translation="MPAPGLLDAVGDTPLIELVPEPPLPSGARLLAKLEFVNPGGSIK
                     DRPVSRILTRALRAGDLGGGRRLLDSTSGNAGVAYAMFGAALGIEVTLVIPGNASRER
                     IERIRAHGARVIETDPIEGYDFALREARRLAEQRPRDYWYANQYANEHNWLAHYESTG
                     PEIVRQVAELTGRPADAFVAGVGTGGTLTGVGRRLREANPDLYLAAIVPETFPGIEGL
                     KPLGAAQDLVPEILDQSLIDERFPMTMDESAETTRWLARRGLFVGPSSGAYVRAAIRL
                     ARSGRFATTVTVLSDTGERYGSTGLWRSTAAGHPRA"
     gene            complement(6826..8280)
                     /gene="moeB"
                     /locus_tag="VD788_11660"
     CDS             complement(6826..8280)
                     /gene="moeB"
                     /locus_tag="VD788_11660"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HXV76961.1"
                     /translation="MSTVTVRIPTPLRTFTNGASEVRVQASTVGEALGVVGQTHDGIL
                     ERVLDRDGRVRGFVNIYLGEHNIRDVGGLDARLDDPSPVISIVPAVAGGAGARQRRLA
                     ELRAAIAEVSPREAHALQQRGAVLIDVRETDETAQGGPAGATRVTRGFLELKIEELVP
                     DPSTPILTMCASGVRSLFAADGLRQLGYRNVRNVASGFSGWKSAGLPVDVPRTLTPDQ
                     RERYGRHLAIPEISEQGQTKLLDAKVLLVGAGGLGSPAAYYLAAAGVGTLGIVDDDVV
                     DRSNLQRQILHQDARVGQPKVDSAGQTLIALNPTVVVERHRTRLTRFNVDEIVPGYDV
                     VVDGSDNFSTRYLVNDACIKHAKPNVHGSVYRFEGQVSVFWPSRANRPGPCYRCLYPE
                     PPPPELAPSCADAGVLGVLPGVIGLLQAVETIKLLLDLGTPLAGRLLQYDALEQRFTM
                     LRVNRDPRCAYCGDGRDFPGYVDYDDTCRAPLAS"
     gene            complement(8277..8762)
                     /locus_tag="VD788_11665"
     CDS             complement(8277..8762)
                     /locus_tag="VD788_11665"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HXV76962.1"
                     /translation="MTDRRSPPPVIWPVELVERLRRLARRGYPHEVCGLLVGRSDRHA
                     TRVARISRARNLATDRLRDRFELDPTDYLRADREARRDGLDVVGVWHTHPDHPARPSA
                     TDLASAWPELSYVIVSVGVDGVRQIRCWRLDGGRFVEQPLAHAPDRFGEPTERTEERR
                     K"
     gene            9060..9710
                     /locus_tag="VD788_11670"
     CDS             9060..9710
                     /locus_tag="VD788_11670"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HXV76963.1"
                     /translation="MIRRYLDPADSLEEVLFGVIMVLTFTLGAGLIVEESEDATTELL
                     LAILGCNVAWGLIDAGMYAMGCILERSRKSRLLESLERAANERDALAIVGRELDERLE
                     PLTSAEERERFYREIIKHLRSVTPERTRLTREVLFGAVATFGLVFASTIPAIVPFLVF
                     HDRFLALRVSNLLLLVMLFLAGFRWARATHANAWLFGTTLLGVGLVLVAIAIALGG"
     gene            9710..10396
                     /locus_tag="VD788_11675"
     CDS             9710..10396
                     /locus_tag="VD788_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76964.1"
                     /translation="MRRARVVPPVAALLACIAVARAADEPPGHAAPRPTEQTWEIYAS
                     LYAYLGSPEPDYLQPTLAADRGALHLEARYNYEDLSTGSFWAGYNLSAGDSVALELTP
                     MLGLVTGRTSGIAAGLAGAVSWRLVECFVEGEYVFDSDDSADSFFYAWSELTLRPVER
                     LRAGLVGQRTRVYDSPRDVQRGILVGIAAGRAELTAHFFNPDDDDPLYVISLGIDFSG
                     VAGGTRATRE"
     gene            complement(10330..>11303)
                     /locus_tag="VD788_11680"
     CDS             complement(10330..>11303)
                     /locus_tag="VD788_11680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HXV76965.1"
                     /translation="AAVTVLIVELRPHRRVAAFVGLALLVTLNHEPFFETLWKQQAET
                     AILLLLALSIVCIRRERDGVAGALLGLAIMLKIYPAALAAYFVVRRRFAALAWCAITL
                     ALVTVATTIVFGLRENAVYFLQILPGMVGEVPIATSENLSLGRYARTIFGIGPGAAEH
                     VVRALAVVLLGVTALVVHRARGREREPLAFGAFVPLFLLVMPNSWVSYQLLLLVPLVA
                     VLKFAAEHPERAAAPLALVAAAGVSLLFYWPCAAPEVPWPCARTPFVLGLFRLPRGLH
                     DLLVDARIVATLLTWAAVLLALRDDRHSRVARVPPATPEKSMPSEMT"
BASE COUNT         1700 a         3954 c         3915 g         1734 t
ORIGIN      
        1 ccgagctcgg ccgactgccg gacgatcgtg tgcccgaggg ggcggactgg ggccgccttt
       61 ccgcacgctg gactcggcgg atcgacgaac ggatcgcgct gctcgagcgc ctgcgcacga
      121 cgctgaccgg ctgcatcggc tgcggctgcc tgtcactcga ccgctgccgg ctcgccaatc
      181 cgggggatcg cctcgcccgt ctcggtccgg gtccgcggta ctgggtggac ggcgggcgcg
      241 gccgagcacc ggtgggcgac gacgagccgg gctgaccgct ccgatcggtc gctcacgagt
      301 cgggtggggc cggcgggcgg ggctcgatcg tgaacgagac gctgtcgggt gagaaatcgc
      361 tgatggtgaa gtactcggac gggatgtcga cgtgctcctg gcggaacggt cgtacgcttc
      421 gacccggctc ggtgtcgctc acgtcgagcg agacgcgcag ctcgtcggga tcgagcagct
      481 cgaaccggtg cttcggcccg gacagcgtca gcgtgatgac ctccgggttg acgtccgtga
      541 tttggagcgt ttccggcaag tcgtggaact cgaccgggac ggacagctgg cgcacgaccg
      601 gctccacgcg ataggccacg gtcaaccaca gcatcgacgc gagcgcgatc gacagcagct
      661 tggtcggcca gtggtgcagc agcgccgggc gcaaccagtc gacggcgccg gcgaacggac
      721 ccgcgctgtg ctcgtcggca gcgccggtct cgcggtggaa cgacccgatc cgcgtctcga
      781 gcgccgccgg gtcgacgacc gcttccatcc ggccccgctg cgccaccgac aacgtccccc
      841 gttcctcgct gacgacgaac accagcgcat cggtcagctc gctcaagccg agagcggcgg
      901 cgtgtcgcgt gcccctcccc gccagctgat cgagccgctc ggacaacggc aagtggacgc
      961 cgaagctcgc cacccggtcg ccgctgacga tcatcgcccc gtcgtgaccg tgggacgacg
     1021 ggtcgaagat cgaagcgatc aacggctccg acacgcgtcc gtcgagcgcg tagccgccgt
     1081 acacgtgcgg atcgagcgac gctcgaccgg gaagcaccac gatcgcgccg atcttccgcc
     1141 gggcgagcgc cgcgagcgat cgcgccagga cctcgaccgt gccgctcgcc ggtccggccg
     1201 aacggccgac gatgccgacc agggcgccca ggcgctcgaa cacgctgcgg atgtccttct
     1261 ggaacaccag gatcagaccg atcaggatcg cggtgaaccc gacctggaac agccacgacg
     1321 tcagcgccat ctgcagttgg cgcgccagca gatagacgaa ggcgacgagt gacagaccga
     1381 cgacgacccc gcggctcgcc cgtcgcagca gccagaggat cgcgaagtag acgcaggcgg
     1441 cgatgatcag gatgtcgatc aggtcggcga cccgaacgac atccctcagc gagctgagaa
     1501 aatcgagcga cgtttcgatc atggcgcacc cgccgggtcc gaacgacgtt acatacatca
     1561 gccgcccgcc gcggagcaag ccggcggcag cgcgaccccc gtgacatctt tttacgacgg
     1621 cgcgttcgca tcgcgcgggc gggggctcgg tacgcggtgc tacagtggga ttttccctcc
     1681 cgcgggagcg gccagcatga gcgtgcgcag catcgtcggc ctgggggcca tcctggtcgt
     1741 cgtcgccggc ctggccgtcc ctcgcctgat cgacgggcgt gccggcgggt cccggcccgc
     1801 gccgtcggcc gccgccccca cccggttggc cgtcgaggtg ctggaggtcg agccgcgccg
     1861 catggccgag cggttggcga cgacgggcac ggtgcgcgcc aacgagtcgg tcgacctcgt
     1921 cagcgagatc gccggcgtcg tccgcacgat ccacttcgac gagggtgcga cggtcgagca
     1981 gggcgagctc ttggtcgaga tcgacgacac cgagctgcag gcccagcgcg atcgcgtgtt
     2041 gtaccggctg cgtctcgccg agaaccgtga ggcacgccag cgcgagctgc gcgagcaggg
     2101 cgtagtcagc gagcaggact acgacgtcgc ggacaacgag ctcaacgtgc tgcgcgccga
     2161 tctgaggctg atcgaggccg agctggccaa ggccgcgatt cgcgcgccgt tttccggggt
     2221 cgtcgggctg cgctacgtca gcacgggcag cctcgtctcg cgcgacaccc tgatcgcgac
     2281 gctgcaggac ctcgatccgg cgaagatcga cttcacgctg cccgaaaagt acgcgggacg
     2341 cgtcggcgcg ggagacccgg tggagttccg cgtccgcggc agcgaccgcg ttcacaccgc
     2401 gcggatctac gccgtcgagc cgcgcatcgt cgccgagacg cgctcgttgt tgctgcgcgc
     2461 cacgaccgcc aacgacgagc gggcgctgtt gcccggcgca ttcgccgacg tgacgctcgc
     2521 cgtcgacgag gtggccgacg cgatcgtcgt accgagcctc gccgtcgtgc ccgagctggg
     2581 gggcaagaag gtcttcgtcg tcgagaacgg gcgcgccgag gcccgacgcg tcgagaccgg
     2641 catccggacc gacaccgaga tccagatcac cgccggtctc cgtccgggcg agcgggtgat
     2701 cgtttcggcg atccagcgtc tcgagcccgg actacccgtc gacgagctgc cgggcgccac
     2761 cggcgcggcg gccgcccgat gagcctctac tcgctgagca tccgccgccc ggtactggcg
     2821 atcgtcatgt cgatcgcgat cctgatcttc ggtctgctcg ggctgtcgtt tctcggcgtg
     2881 cgcgagttcc ccgcggtcga cccaccgctg atcacggtca gcaccgacta ccgcggggcc
     2941 aacgccgatg tcatcgagac gcagatcacc gagccgctcg aagagtcgat caacgccgtg
     3001 gccggcatcc gcacgatgac ctcgaccagc cgcgaggggc ggagcacgat catcgtcgag
     3061 ttcggtctcg aatcggacct cgagcgcgcc gccaacgacg tgcgcgacgc cgtctcccgc
     3121 gcgctgggca acctgccgcc cgacgtcgag ccgccgcagg tcagcaaggc cgatgccgat
     3181 gcgagcccga tcgtgcaggt cacgctcggc agcgacacgc gcgactcgct cgagctgacc
     3241 cgcctcgccg acgagctgtt cgccgaacgg ctgcagacca ttcccgaggt cgcgcgagtg
     3301 gacatctggg gcgacaagac ctacgccatg cgtctgtggg tcgatccgca gaaactggcg
     3361 gcctaccgcc tgacgccgct cgacctccgc cgcgccgttg agcgcgagaa cgtcgagctg
     3421 ccggccgggc ggatcgaggg cggcacgatc gagctcccgg tgcgcgtgct cagccggctg
     3481 tcgacgccgg acgagttcaa cgcgatgatt ctcaaggagg acgccggtgg cgtcgtccgc
     3541 ctgcgcgacg tcggacaggc cgtgctcgga ccgcgcaacg agcggacggt gctgaaaaaa
     3601 gacggggtgc cgatggtcgc cgtcgtgctg cggccgcagc ccggcgccaa ctatatcgag
     3661 atcgccgacg agttctatcg ccgggtcgag cagatccggc gcgagctgcc cgccgacgtg
     3721 accgtcgagt acggcttcga caccaccgac ttcatccgca agtcgatcgc cgaagtgggt
     3781 cagacgatcg tgctggcgct gctgctcgtc gtgtcgatca tcttcctctt cctgcgcgac
     3841 ctgcgcacga cggtcatccc gctgctcgtc atcccggtct ctctggtcgg cgcgttcttc
     3901 gtgatgtacg cgctcgggtt ctcggtcaac gtgctgaccc tgctcgcgct cgtgctcgcg
     3961 atcggtctcg tggtcgacga cgcgatcatc gtgctggaga acatctacgc caagatcgag
     4021 gccgggttgg cgccgttgca ggccggggtg gccggtacgc gcgagatctt cttcgccgtc
     4081 gtcgccacca cgctcgcgct cgtcgcggtg ctgctgccgg tgatgttcct cggcgggctg
     4141 accggacgac tgttccgcga gttcggcgcc acgctggcgg gtgcggtgat catctcgtcg
     4201 ttcgtcgcgc tgagcatgac gccgatgttg tgcacgcgca tcctgcgccg ctacgagggt
     4261 cactcgcgct tccaccgggc gaccgagccg ttcttccgtt cgctggccgc ggcgtatcgc
     4321 gacacgctcg agcggctgat gacccggcgc tggctcgccg tgccgttgat tgccggctgc
     4381 ggcgggctga tcacactgtt cttctggctg ttgccggcgg agctggcgcc gatcgaggac
     4441 cgcagcgggc tgcggctgag cgttcgcggg ccggagggcg cgacctatcc ctacatgggt
     4501 cgcgtgatgg acgatctggg cgccgcggtg gccgaggcgg tgcccgaggc cgagcagatc
     4561 atctgggtca cctcgcccgg cttcggcgcc agctcgtcgg tcaattccgg cttcctgcgt
     4621 ctgcgtctcg tccctcccga gcaacgcgaa cgcagccaga tggagatcgc cgacgcgttg
     4681 ggcgccgtca cccggcggct gaccggcgcg cagatcttcg tcacgcagga gccgacgatc
     4741 gccgtcggcc ggcggcgcgg gctgccggtg cagttcgtgc tgcaggcacc cacgctcgag
     4801 cggctgaagg acgtcctgcc gcgctttctc gacgaggccc ggggcgatcc ggcgttctcg
     4861 gaagtcgacg tcgacctgct gttcgacaag cccgagctga ccgtcgacat cgaccggccg
     4921 cgagccaggg acctcgggat ctccgtgctc gacgtggcgc agaccgtaca gctcgcgttg
     4981 agcgagcagc gactcggctt cttcgtgatg gacggcaaac agtacgaggt gatcgggcag
     5041 gtcacctcgg acaaccgcaa cgagacgatc gacctgcgca atctctacgt cgcctcgcgc
     5101 agcggcgcgc cggtgctgct cgacaagctc gtgaccatcg ccgagcgttc gagcccgccg
     5161 cagctctacc gcttcgaccg ctacgtctcg gcgacggtgt cggcggggct cgccccgggg
     5221 ctgaccatct cccaggggat cgacgcgatg caggcgatcg ccgaccggct gctcgacgac
     5281 acgtttcaca cgacgttggc cggcgagtcg cgcgattatc aagaaagctc gaacacgctg
     5341 gtcttcatct tcgggctggc gatcgtgctg ctctacctcg tgctggcggc gcagttcgag
     5401 agcttccgcg atccgttcac gatcatgctg accgtgccgc tggcgctcgc cggcgcgttg
     5461 ttcgcgctgt ggtggtatgg gctgacgctg aacgtgttca gccagatcgg catgatcatg
     5521 ctgatcggtc tggtgacgaa gaacggcatt ctgatcgtcg agttcgccaa ccagcgacgc
     5581 gcggcaggcc gcccgttgat cgaggcgacg gtcgaggccg ccgcggcgcg gctgcggccg
     5641 gtgttgatga ccagcctgtc gacggtgctc ggcatcctgc cgatcgcgct gtccttgggc
     5701 gcggggtcgg cgagccgcgt gccgatgggc gtggcggtga tcggcggtct gacgttcggc
     5761 acgctcttga cgctgttcgt cgtgcccgcg atgtacacct tcctcaccgc gaggacactc
     5821 gtccgcgtcg gtgccgacga cctcgaaggg cacgaagccg cctgaccgac catcagtcac
     5881 gcccgcgggt gacccgctgc cgtcgatcgc cacagaccgg tcgagccgta gcgctcgccg
     5941 gtgtcgctca acacggtgac cgtcgtcgcg aaacgcccgc tccgcgcgag ccggatcgcc
     6001 gcgcgcacgt acgcccccga cgagggcccg acgaataaac cgcgccgggc gagccagcgc
     6061 gtcgtctcgg cgctctcgtc catcgtcatc gggaaccgct cgtcgatcaa cgactggtcg
     6121 aggatctcgg gcacgagatc ctgcgcggcg ccgagcggct tgagcccctc gatgccggga
     6181 aaggtctcgg ggacgatcgc ggccaggtac aggtccggat tcgcctcgcg cagacgccgc
     6241 ccgaccccgg tcagcgtgcc gccggttccg acacccgcga cgaacgcgtc ggcgggccgg
     6301 ccggtcagct cggcgacctg cctcacgatc tcgggcccgg tcgactcgta gtgcgccagc
     6361 cagttgtgtt cgttcgcgta ctggttggcg taccagtagt cccgcggtcg ctgttccgcc
     6421 agccgcctcg cctcgcgcag cgcgaaatcg tagccttcga tcgggtcggt ctcgatgacc
     6481 cgggcaccgt gcgcgcggat ccgctcgatc cgttcccgac tggcgttgcc cgggatcacc
     6541 agcgtcacct cgatgccgag cgcggccccg aacatcgcat aggccacgcc cgcgttgccc
     6601 gaggtcgaat cgagcaggcg ccgcccgccg ccgagatcac cggcccgcag cgcgcgggtc
     6661 aggatgcggc tgaccggccg gtccttgatc gagccgccgg ggttgacgaa ctcgagcttg
     6721 gccaacaacc gcgcgccgct cggcaacggc ggctcgggca ccagctcgat cagcggtgtg
     6781 tcgccgacgg cgtcgagcag gccgggcgcg ggcacctgcg cctcctcagc tcgccaacgg
     6841 tgcgcggcac gtgtcgtcgt agtcgacgta gcccgggaag tcacggccgt ccccgcagta
     6901 ggcgcagcgc gggtcgcggt tgacccgcag catggtgaag cgctgctcga gcgcgtcgta
     6961 ctgcaacaac cggccggcga gcggcgtgcc gaggtcgagc agcagcttga tcgtctcgac
     7021 cgcctgcaac agcccgatca cgccgggaag cacgccgagc acgcccgcat cggcgcacga
     7081 cggtgccagc tcgggcggcg gcggctcggg atacagacag cggtagcacg gccccggccg
     7141 gttcgcgcgg gagggccaga agacgctgac ctgtccctcg aagcgataga cggatccgtg
     7201 gacgttcggc ttcgcgtgct tgatgcaggc gtcgttgacc agataacgcg tggagaaatt
     7261 gtcgctgccg tcgacgacga cgtcgtagcc ggggacgatc tcgtcgacgt tgaaccgggt
     7321 cagccgcgtc cgatgacgct cgacgacgac cgtcgggttg agcgcgatga gcgtctgacc
     7381 cgccgagtcg accttcggct gtccgacgcg ggcgtcctgg tgcaggatct ggcgctggag
     7441 gttcgaccgg tcgacgacgt cgtcgtcgac gatgcccagg gttcccacac cggcggccgc
     7501 caggtagtac gccgccggcg agccgagacc accggccccg accagcagga ccttcgcgtc
     7561 gagcagcttg gtctgtccct gctcgctgat ctccgggatc gcgaggtggc gcccgtaacg
     7621 ctcgcgctga tcgggcgtca gcgtgcgcgg cacgtcgacg ggcaggcccg cgctcttcca
     7681 gccgctgaac cccgaggcga cgttgcggac gttgcggtac ccgagctggc gcaggccatc
     7741 ggcggcgaac agcgagcgca ctccgctcgc gcacatcgtc aggatcgggg tggacggatc
     7801 gggcaccagc tcttcgatct tcagctcgag aaaaccgcgg gtgacgcgcg tcgcaccggc
     7861 gggcccgccc tgggccgtct cgtcggtttc gcggacgtcg atcagcaccg ccccgcgctg
     7921 ctgcaacgcg tgggcctcgc gcgggctgac ctcggcgatc gcggcgcgca gctccgccag
     7981 ccgtctctgt ctggcgccgg ccccgcccgc gacggccggg acgatcgaga tcaccggcga
     8041 cgggtcgtcg agccgcgcgt cgagcccgcc gacgtcgcgg atgttgtgct cgccgaggta
     8101 gatgttgacg aaaccgcgga cccgaccgtc tcggtcgagc acgcgctcga ggatcccgtc
     8161 atgggtctgg ccgacgacgc ccaacgcctc gccgaccgtg ctcgcctgca cccgcacctc
     8221 gctcgcaccg ttggtgaatg tgcgcagcgg cgtcgggatt cggaccgtga ccgtgctcat
     8281 tttcttctct cctctgtccg ttcggttggt tctccaaacc gatctggggc atgtgccagt
     8341 ggctgctcga cgaaccgccc gccgtcgaga cgccagcagc ggatctgccg cacgccgtcg
     8401 acgccgacgg acacgatcac gtagctcagc tccggccagg ccgaggcgag atcggttgcc
     8461 gaaggccgtg ccgggtgatc ggggtgggtg tgccagacgc cgacgacgtc gaggccgtcc
     8521 cgcctcgcct ctcgatcggc gcggaggtag tcggtcggat cgagctcgaa gcgatcgcgc
     8581 aggcgatccg tcgccaggtt ccgcgcgcgg gagatgcgcg cgacccgtgt cgcgtgccgg
     8641 tcggaccgac cgacgagcag tccgcagacc tcgtgcgggt aaccccggcg ggcgaggcgc
     8701 cgcaatcgct cgaccagctc gaccggccag atcacgggcg gcggggaacg ccggtccgtc
     8761 actccgagga ctcctgttcg caccacgggc gacccccaaa agcaagaacg ccgggctgat
     8821 cgcccggcgt tcgttcggtc atcgcgatta gctggggccg atcagacagg cgtctcccca
     8881 ccgggcggat tgtcgccagt acagcaacag cgagtctggg tccctcgtcc gatcatcacc
     8941 cgattcttgt actccggttc gacgcggctg tcaaacggct cggtgattcg accgggatcg
     9001 cgtcgatccg ggtttcgcgc tagcctggcc gggacgggcc gttacgcgtc ggaggaccga
     9061 tgatcagacg ttacctcgac ccggcggaca gcctcgagga agtgctgttc ggcgtcatca
     9121 tggtgctgac cttcacgttg ggcgccggtc tgatcgtcga ggaaagcgag gatgccacca
     9181 ccgagctgct gctcgccatc ctcggctgca acgtcgcctg gggtctgatc gatgccggga
     9241 tgtacgcgat ggggtgcatc ctcgagcgga gtcgcaagtc gcgactgctc gagtcgctcg
     9301 agcgagcggc caacgaacga gacgcgctgg cgatcgtcgg gcgcgagctg gacgaacggc
     9361 tcgagccgct gacctcggcc gaggagcgcg agcggttcta ccgcgagatc atcaaacacc
     9421 tgcgcagcgt cacccccgag cgcacccggc tgacgcgcga agtcctgttc ggggcggtcg
     9481 ccacgtttgg tctcgtgttc gcctcgacga ttcccgcgat cgtgccgttt ctcgtctttc
     9541 atgaccgctt cctcgcgctg cgcgtgtcga acctgctgct gttggtcatg ctgttcctcg
     9601 ctggcttccg ctgggctcgg gcgacgcacg ccaacgcttg gttgttcggc acgacgctgc
     9661 tcggagtggg gttggtgctc gtcgcgatcg cgatcgcgtt gggaggttga tgcgacgcgc
     9721 ccgcgtggtg ccgccggtcg cggcgctgtt ggcctgcatc gccgtcgctc gcgcggccga
     9781 cgaaccaccg ggtcatgccg cgccgcgacc caccgagcag acgtgggaga tctacgcttc
     9841 gctctacgcc tatctcggct cgccggagcc cgactacctg cagccgacgc tcgcggccga
     9901 ccgcggcgcg ctgcacctcg aggcgcgcta caactacgag gatctgtcga ccgggtcgtt
     9961 ctgggccgga tacaacctga gcgccggcga ctcagtcgcg ctcgagctca cgccgatgct
    10021 cggcctggtc acgggacgca cgagcgggat cgccgcgggc ctcgcgggtg ccgtctcctg
    10081 gcggctcgtc gagtgcttcg tcgagggcga gtacgtcttc gacagcgacg attccgccga
    10141 ttcgttcttc tatgcatggt ccgagctcac gctgcgtccg gtcgaacgac tgcgcgcggg
    10201 cctcgtcggg cagcggacgc gggtctacga cagcccgcgc gacgtccagc gcgggatcct
    10261 cgtcggcatc gccgccggtc gtgcggagct caccgcccac ttcttcaacc cggacgacga
    10321 cgacccgctc tacgtcatct cgctcggcat cgacttctcg ggggtcgcgg gcggcacgcg
    10381 cgccacccgg gaatgacggt cgtcgcgcag cgccaacagc accgcggccc aggtcagaag
    10441 cgtcgcgacg atcctggcgt cgaccagcag atcgtgcagg ccgcggggca gtcggaacag
    10501 cccgaggacg aacggcgtgc gcgcgcaggg ccacggcacc tcgggcgcgg cgcacggcca
    10561 gtagaacagc agcgaaacgc cggcggcggc caccagcgcc aacggtgccg cggctcgctc
    10621 ggggtgctcg gccgcgaact tcagcacggc gacgagcggc accaacagca gcagctggta
    10681 gctgacccac gagttgggca tcaccagcaa gaacagcggc acgaacgccc cgaacgcgag
    10741 cggctctcgc tcgcgccccc gcgcgcgatg gacgacgagc gccgtcacgc cgagcaggac
    10801 gacggcgagc gctcgcacga cgtgctcggc cgcgccgggg ccgatgccga agatcgtccg
    10861 ggcgtagcgg ccgagcgaca ggttctcgct ggtcgcgatc ggcacctcgc ccaccatccc
    10921 cggcaggatc tgcaagaagt agaccgcgtt ctcccgcaag ccgaagacga tcgtcgtggc
    10981 gacggtcacc agcgccagcg tgatcgcgca ccaggcgagg gcggcgaacc ggcggcggac
    11041 gacgaaatac gccgccagtg ccgccggata gatcttgagc atgatggcca ggccgagcag
    11101 cgcaccggcg acgccgtcgc gctcgcgccg gatgcagacg atcgacagcg cgagcaacag
    11161 caagattgcc gtctcggcct gctgcttcca cagcgtctcg aagaacggtt cgtggttcag
    11221 cgtgacgagc agcgccaatc cgacgaacgc cgcgacccga cggtgcggtc gcagctccac
    11281 gatcagcacg gtgacggccg ccg
//
LOCUS       DASPGN010000402         5719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_73080, whole genome shotgun sequence.
ACCESSION   DASPGN010000402 DASPGN010000000
VERSION     DASPGN010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5719
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73080"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VD788_11685"
     CDS             complement(<1..1485)
                     /locus_tag="VD788_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXV76966.1"
                     /translation="MPPDRATPDERWSRLVECVRRDSTVSFAEQLSRYRAIDGDRESR
                     GAPLAAWVPEGPRPATSNAAVVLRAVGLDSFAGLHAWSVADRVAFWQLVIERLAIRFD
                     RPPRRISGRPDRPTAPDWLSGARMNCVDSCFAAEGDRAAIVWGDERDPLPRTVTYREL
                     RGLVDRVAGGLRAHRFAPGARIALYMPMNAACVAAYLGIILAGCQVVSIADSFPPPEL
                     ARRLRIARADGIVTVRRFIRAGKTIGLYDHVLAAAAPRAIVIDDDPKAPLARPDDLGW
                     TELLADAADAGAVHGPPSTPTTVLFSSGTTGDPKAIPWTHLTAIKAAMDGHFHQDIRP
                     TDVVAWPTNIGWMMGPWLIFAGLINRATIALYEGSPVGEGFLRFVGDARVSVLGVVPS
                     LVRAWRSRGEDRPVLADVELFSSTGEPSNVADYLWLMSRTAYRAPVIEYCGGTEIGGG
                     YLTGSVLQPAVPATFTTPALGLDLVLIGDDGRIVSGRGGGEAFLV"
     gene            complement(1491..2186)
                     /locus_tag="VD788_11690"
     CDS             complement(1491..2186)
                     /locus_tag="VD788_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HXV76967.1"
                     /translation="MSEFSPEDAPRPVDLVAFDVDGTLIREDDGLTVWEVLNREFVGD
                     CRINRERHRMFLDGKLSYAEWVALDIGGWQAAGATRERIVAAFASLAPIDGARPALDT
                     LAAFGVKLVVISGTLDLLLETLLPDAPFDEVYCNHIGFDEFGRISHWAATPFDNRGKE
                     KLLRAIALREGIPLERCGFVGDSTNDRWIARAAGLSVALNPRCDELERAADVVLRARD
                     LRDVLPCLLGRGS"
     gene            complement(2183..4357)
                     /locus_tag="VD788_11695"
     CDS             complement(2183..4357)
                     /locus_tag="VD788_11695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HXV76968.1"
                     /translation="MPKPRVSAAGGLAAIGYTLKKGREAGGLWRLYTRLRSRNACKTC
                     AFGMGGQRGGMVNEQGRWPEVCKKSIQAQAGDMQAPLTEAFFARHSLAELERFTSRQL
                     EHAGRLVFPIVADARDVRFRRIGWDEALARLSDDLRAAPPEQTFVYASGRSSNEAAFL
                     LQMLARAYGTPNIHNCSSYCHQASGVALSRMIGSGTATVVLEDLDHADCMLLVGANPA
                     SNHPRLIVKMVELRKRGGRVIVINPVRELGLERFRIPSKPGSLLFGSTVSDLYLQPHV
                     GSDITVFRALLKGLLERSAEDRDYLARYAEGWEAVERDARTRSWQSLLDGCGLRRDEI
                     ERAVDILAAARRGIALWAMGLTHHEHGVDNVLALGNLAAARGWVGRAGSGLMPIRGHS
                     NVQGVGSVGFTPALKRAFAEKMAELYGIDPRPAGMDTFASMTAAEQGKVRVALMLGGN
                     LWGSNPDLGWATRAMRRIGTTAYITTKLNPGHVHGRGSFNLVLPVLARDEETQPTTQE
                     SMFNFVRLSDGGAAAPSGEAISEVALVASLARRLLPDAGFPFERMTSHATIRRAIADV
                     VPGYRAIGRIDESRNEFQIEGRTFHEPRFSTPSGKLVLHVPRETDWRPADGAFRLMTL
                     RSEGQFNTVVYEDEDLYRGTTRRDVVMMHPRDGERLGVREGRAVSVETEVGALPAVVA
                     FIDIAPGNLAMYYPEANAIVPRRVDRDSGTPAFKSVSARIVV"
     gene            4396..5205
                     /gene="mutM"
                     /locus_tag="VD788_11700"
     CDS             4396..5205
                     /gene="mutM"
                     /locus_tag="VD788_11700"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HXV76969.1"
                     /translation="MPELPEVETVARQLEPRLAGRRVVALRILDPRLREQPTPRLRGR
                     RVTEVGRAGKRVVVGFDGNGERRGLWLAVHLRMTGRLIWADEGVPIDSRSLRARFRLD
                     RGNLLFYDTRRFGTFFWTAERSAAEPQALDPLDPRLDAARLARLLAGGSQPLKVWLLR
                     QDRLVGLGNIYASEICHVARLSPTRGVDTLDATETGRLLSATRRVLRRAIRSCGTTFS
                     DYQDANGLAGSYQHHLRVYGREGAACRRCAATIRRIVQQQRGTFYCPDCQR"
     gene            complement(5225..>5719)
                     /locus_tag="VD788_11705"
     CDS             complement(5225..>5719)
                     /locus_tag="VD788_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV76970.1"
                     /translation="ARAAAPERTLAVHSFSKAFGMAGNRCGYVVGPASVLAQVEKATT
                     HTVYCAPHAAQLAALGALGRAGERWLADARERYARAGRRAAGRLGVPPPAGGTFLFLD
                     VAAQLDGSGLFGFLDRCVTNGLLLAPGPSFGPYPSHVRLCFTCSEPAVIERGVEVLAR
                     LIGR"
BASE COUNT          869 a         2025 c         2008 g          817 t
ORIGIN      
        1 cacgagaaac gcctcgccgc cgccgcggcc gctgacgatc cgcccgtcgt cgccgatcag
       61 caccaggtcg agccccagcg ccggcgtggt gaaggtggcg ggaaccgcgg gctgtagcac
      121 ggaacccgtg aggtaaccgc cgccgatctc cgttcccccg cagtactcga tcaccggggc
      181 gcggtaggcg gtccgactca tcagccacaa gtagtccgcg acgttcgacg gctcgcccgt
      241 gctgctgaac aactcgacgt cggcgagcac cggtcgatcc tcgccgcggg agcgccaggc
      301 gcgcaccaac gacggcacga cgcccaacac gctgacacgc gcgtcgccga cgaagcggag
      361 gaagccttcg ccgacgggtg agccttcgta cagcgcgatc gtcgcgcggt tgatcaggcc
      421 ggcgaagatc agccacgggc ccatcatcca accgatgttg gtcggccagg cgacgacgtc
      481 ggtcgggcgg atgtcctgat ggaagtggcc gtccatcgcc gccttgatcg cggtcaggtg
      541 ggtccacggg atcgctttcg gatctccggt cgttcccgac gaaaacagca cggtcgtcgg
      601 ggtggacggc gggccgtgca ccgccccggc gtccgcggca tcggcgagga gctcggtcca
      661 gcccaggtcg tcggggcgcg cgagcggtgc cttcgggtcg tcgtcgatga cgatcgcccg
      721 cggtgccgcc gcggccagca cgtggtcgta gagcccgatc gtcttgccgg cgcgaatgaa
      781 gcgccggacg gtgacgatgc cgtccgcccg ggcgatgcgc agcctgcgcg cgagctcggg
      841 aggcggaaag ctgtcggcga tcgacaccac ctggcaaccg gcgaggatga tgccgagata
      901 ggccgcgacg cacgcagcgt tcatcggcat gtagagcgcg atgcgcgcgc cgggcgcgaa
      961 gcgatgggcg cggagaccgc ccgccacgcg atcgacgagt cctcgcagct cgcggtaggt
     1021 cacggttcgc ggcagcgggt cccgctcgtc gccccagacg atcgccgcgc gatcgccctc
     1081 ggccgcgaag cagctgtcga cgcagttcat ccgtgcgccg ctcagccagt cgggggcggt
     1141 cggacggtcg gggcgccccg aaattcgtcg gggcggacga tcgaagcgga tcgcgagtcg
     1201 ttcgatgacg agctgccaga acgccacccg gtcggcgacg gaccaggcgt gaaggccggc
     1261 gaacgagtcg aggccgaccg cgcgcaacac gacggcggcg ttcgacgtcg ccggccgtgg
     1321 gccctcgggc acccacgcgg cgagcggagc ccccctgctc tcacggtcgc cgtcgatcgc
     1381 gcggtagcgc gacaactgct cggcgaacga cacggtcgaa tcgcgccgga cgcactcgac
     1441 gagccggctc cagcgttcgt cgggcgtcgc gcgatccggc ggcatccgcg tcagctcccg
     1501 cgaccgagca ggcagggcag gacgtcgcgc agatcgcggg cgcggagcac gacgtcggcc
     1561 gcgcgctcga gctcgtcgca ccgcgggttc agcgccacgc tcagacccgc ggcccgggcg
     1621 atccaccggt cgttcgtgct atcgccgacg aagccgcagc gctcgagcgg gatcccctcg
     1681 cgcagcgcga tcgcgcgcaa caacttctcc ttgccgcggt tgtcgaatgg tgttgccgcc
     1741 cagtgcgaga tgcgaccgaa ctcgtcgaag ccgatgtgat tgcagtagac ctcgtcgaac
     1801 ggggcgtccg gcagcagggt ctcgagcagc aggtcgagcg ttcccgagat gacaaccagc
     1861 ttcactccga atgcggcgag cgtgtcgagc gccggtcgcg cgccgtcgat cggtgccagc
     1921 gacgcgaacg cggcgacgat ccgctcccgc gtcgcaccgg ccgcctgcca gccgccgatg
     1981 tcgagggcga cccactcggc gtagctcagc ttgccgtcga ggaacatgcg gtggcgttcg
     2041 cggttgatcc ggcaatcgcc gacgaactcg cgattgagca cctcccacac ggtcagcccg
     2101 tcgtcctcgc ggatcagcgt cccgtcgacg tcgaacgcga ccaggtcgac cggccgcggc
     2161 gcgtcctcgg gcgagaactc gctcaaacca cgatccgcgc ggataccgac ttgaacgccg
     2221 gcgtgccgga atcgcgatcg acgcgtcgcg gcacgatcgc gttggcttcg gggtagtaca
     2281 tcgcgaggtt acccggtgcg atgtcgatga aggcgacgac cgcgggcagc gcgccgacct
     2341 cggtttcgac cgacaccgcg cggccctctc gaacgcccag tcgttcgccg tcgcgcgggt
     2401 gcatcatcac gacgtcgcgt cgggtcgtgc cgcggtagag atcctcgtcc tcgtagacca
     2461 ccgtattgaa ctgcccctcc gagcgtagcg tcatcagccg gaaggcgccg tccgcgggac
     2521 gccagtccgt ctcgcgcggt acgtgcagga cgagcttgcc cgagggagtc gagaagcgag
     2581 gttcgtggaa ggtacgcccc tcgatctgga attcgttcct cgactcgtcg atccgaccga
     2641 tggcgcggta gcccggtacg acgtcggcga ttgcacgccg gatcgtcgcg tgactcgtca
     2701 tccgctcgaa cgggaacccg gcgtcgggca acaaccgccg ggccaacgag gcgacgagtg
     2761 cgacttcgct gatcgcctcg cccgacggcg cggcggcgcc gccgtcggac aaccggacga
     2821 agttgaacat gctctcctgg gtcgtgggct gggtctcctc gtcgcgcgcc agcaccggca
     2881 gcaccaggtt gaagctgccg cggccgtgga cgtgtcccgg attgagcttg gtcgtgatgt
     2941 aggccgtcgt tccgatccgg cgcatggcac gggtggccca gccgagatcg ggattcgagc
     3001 cccacaggtt gcccccgagc atcagcgcca cacgcacctt gccctgctcg gccgcggtca
     3061 tcgaggcgaa ggtgtccatg ccggccggcc gcgggtcgat tccgtacagc tcggccatct
     3121 tctcggcgaa ggcccgtttg agagccggag tgaacccgac cgatccgacg ccctggacgt
     3181 tgctgtgccc gcgtatcggc atcaggccgc ttcccgcacg gccgacccac ccgcgggcgg
     3241 ccgcgaggtt gccgagcgcc aggacgttgt ccacgccgtg ctcgtggtgc gtcagcccca
     3301 tcgcccacag cgcgattccc cgccgggcgg cggcgaggat gtcgacggcg cgctcgatct
     3361 cgtcacgccg cagcccgcag ccgtcgagca acgactgcca gctccgcgtc cgtgcgtcgc
     3421 gctcgaccgc ctcccagccc tcggcgtagc gcgcgaggta gtcccggtct tcggccgagc
     3481 gctcgagcag ccccttgagc aaggcgcgaa agacggtgat gtcggagccg acgtgcggct
     3541 gcaggtagag gtcgctgacg gtcgagccga acagcaggct tcccggcttg gacgggatcc
     3601 tgaaccgctc gagtcccagc tcgcgcaccg ggttgatcac gatcaccctg ccgccgcgtt
     3661 tcctcagctc gaccatcttg acgatcagcc gcggatggtt cgaggccggg ttggcgccga
     3721 cgagcagcat gcaatcggcg tgatccaggt cctcgagcac gacggtcgcc gtgccgctgc
     3781 cgatcatccg agagagcgcg acgcccgaag cctggtgaca gtacgaggag caattgtgga
     3841 tgttcggcgt tccgtaagcc cgcgccagca tctgaagcag gaacgccgcc tcgttcgacg
     3901 agcggcccga ggcgtagacg aaggtctgct cgggcggagc ggcccgtaga tcgtcgctca
     3961 gacgggcgag tgcctcgtcc cagccgatcc tgcggaaacg aacgtcgcgc gcgtcggcca
     4021 cgatcgggaa gaccagtcgg ccggcgtgtt ccagctgacg ggaggtgaag cgctcgagct
     4081 ccgccagcga atgacgcgcg aagaacgcct cggtcagcgg cgcctgcatg tcgccggcct
     4141 gggcctggat cgatttcttg cagacctccg gccaccggcc ctgctcgttg accatcccac
     4201 cgcgctggcc gcccatgccg aacgcacagg tcttgcaggc gttgcgggac cggagtcgcg
     4261 tgtacaggcg ccacagccca ccggcctcgc ggcccttctt gagcgtgtag ccgatcgcgg
     4321 ccaacccgcc tgcggcggag actctcggtt tgggcatcgc actcctcggc gtcccgccca
     4381 tgatacttta gcccggtgcc cgagcttccg gaggtcgaga cggtggcgcg ccagctcgag
     4441 ccgcggctgg cgggtcgtcg cgtcgtcgcc ttgcggatcc tcgatcctcg gctgcgcgag
     4501 caaccgacgc cgcggctccg cgggaggcgc gtcaccgagg tcggccgcgc cgggaagcgg
     4561 gtcgtggtcg gcttcgacgg caacggcgag cggcgcggcc tgtggctcgc cgtccacctg
     4621 cggatgaccg gtcggctgat ctgggccgac gagggcgttc ccatcgactc gcgttcgctg
     4681 cgcgcccgct ttcgtctcga tcgcgggaat ctgctgttct acgacacgcg tcgcttcggc
     4741 acgttcttct ggaccgccga gcgaagcgcg gccgagcccc aggcgctcga cccgctcgat
     4801 ccgcggctcg atgccgcgcg tctcgcccgg ttgctcgccg gcggctccca gccgctcaag
     4861 gtttggctgc tgcgtcagga ccggctcgtc gggctgggaa acatctacgc gtccgagatc
     4921 tgccacgtcg cgaggctgtc gccgacgcgc ggcgtcgaca cgctcgacgc gaccgaaacg
     4981 ggtcgcctgc tgagcgcaac gaggcgagtt ctgcgtcgcg cgatccgcag ctgcgggacg
     5041 acgttctccg actatcagga cgccaacggc ctcgccggat cctatcagca ccacctgcga
     5101 gtgtacggac gcgagggcgc cgcgtgccgc cgctgcgcgg cgacgatccg acggatcgtc
     5161 cagcaacagc gcggcacctt ctactgtccg gactgccaac gctgagagcg ggggagcgct
     5221 ccgctcagcg accgatcaac cgcgcgagca cctcgacgcc gcgctcgatc accgcgggct
     5281 cgctgcaggt gaagcacagt cgcacgtggg acgggtaggg gccgaagctc gggcccggcg
     5341 cgagcagcag gccgttcgtc acacagcggt cgagaaaacc gaagagcccg ctgccgtcga
     5401 gctgcgcggc gacgtcgaga aagaggaagg tccctcccgc aggcggtggg acgccgaggc
     5461 gaccggccgc gcgccggccg gcgcgggcgt agcgctcgcg ggcgtcggcg agccaacgct
     5521 ctccggcgcg gcccagggcg ccgagcgccg cgagctgggc cgcgtgcggc gcgcagtaga
     5581 cggtgtgggt cgtcgccttc tcgacctggg ccagcaccga cgccggtccg acgacgtagc
     5641 cacagcgatt gcccgccata ccgaacgcct tggagaacga gtggacggcc agcgtccgtt
     5701 cgggcgccgc ggcccgtgc
//
LOCUS       DASPGN010000403         4681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_775996, whole genome shotgun sequence.
ACCESSION   DASPGN010000403 DASPGN010000000
VERSION     DASPGN010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775996"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VD788_11710"
     CDS             <1..364
                     /locus_tag="VD788_11710"
                     /inference="COORDINATES: protein motif:HMM:NF018531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HXV76971.1"
                     /translation="RARMSPFKQTRALAEDEIARLFDAARTTLDEWTDRLRREAGDGF
                     PQHVTAFRREMAVHGRYRRPCPDCGAPVQRIVYSGNEANYCAVCQTGGKLLADRALSR
                     LLKSDWPKTIEELEHRSR"
     gene            complement(397..1503)
                     /locus_tag="VD788_11715"
     CDS             complement(397..1503)
                     /locus_tag="VD788_11715"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HXV76972.1"
                     /translation="MSTPPLTIGIEEEYQIIDPATGQLTSYIQEMLEQGRVVLEGQIK
                     PEFLQSQVEVGSRICSDVQQARSELLRLRGTVCRMAEAKGRRVAAASTHPFSQWVSQQ
                     RSAGERYTKHEHDMGDVARQMLVFGMHVHIGIEDPELRIDVMNQARYFMPHLLALSTS
                     SPFWHGRDTGLKSYRTIVMGNLPRAGLPPSFNSWAEYQRFLAILIETNCIDEPTKIWW
                     DIRPHPNYPTLEFRFADICTRVEEAVCIAGLIQAIVAKLIKLRRGNVRWRDYRRNLIT
                     ENKWRAVRHGIDGLLIDFGKRKELPVRSLIVELLDWVDDVVEELGSREEVAYAHTILR
                     EGTSADRQLSVYRETGDFKRVVDLVIAETTIGCR"
     gene            1706..2692
                     /locus_tag="VD788_11720"
     CDS             1706..2692
                     /locus_tag="VD788_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76973.1"
                     /translation="MLVSSCVGFVWWLNRPGELLEPARLRGSDTTAYVEWTLRLDDPG
                     TGALVEDLLDGLSELQEQIGAPLPPGIADVLNEMQNRRNEREFRQLFPCVVAWSVVGD
                     DGGGEDLHLVSVSIEKLGNRMVFIDWVMGLFVGRSPEVDVVRHGGEKIYVLREPGSAF
                     FLRRSDFIFTSDADTARRAVDRLVAQREETEAPSGIEPLLADLPPEHPLRGASLNRND
                     EIRRLFEMVTGDDERGVLDALDWDRVRAMTFSGGFTEQSSFAALVTLDTDAAAWHADE
                     LAALERVVRRVFELDASATQVALDRLDDRIVVRIELDDLPSLVRRRLALDGR"
     gene            complement(2701..3327)
                     /locus_tag="VD788_11725"
     CDS             complement(2701..3327)
                     /locus_tag="VD788_11725"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HXV76974.1"
                     /translation="MHHERFLTNGPQGSRSPRVVLAHGAGAPMDSPFMNRIADGLGRG
                     GLSVVRFEFPYMARRREDGKRRAPDRGDVLRRSWLEVIERFAPARRLVIGGKSMGGRI
                     ASLVADEAGVAGLVCLGYPFHPAGRPERLRAAHLAQIATPTLIVQGTRDPLGNREEIA
                     GYVLSERVRVEFIEDGDHSLEPRKRSGSDKERSLEQAISAVTAFVAAR"
     gene            3598..3831
                     /locus_tag="VD788_11730"
     CDS             3598..3831
                     /locus_tag="VD788_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXV76975.1"
                     /translation="MAKRIGIVEVASDCCKGCGLCVASCPTGSLVLGDGWNRLGYHPV
                     GFVVDSGCTGCGVCFYACPEPAALRVLTADRAA"
     gene            3848..>4681
                     /gene="vorB"
                     /locus_tag="VD788_11735"
     CDS             3848..>4681
                     /gene="vorB"
                     /locus_tag="VD788_11735"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HXV76976.1"
                     /translation="MGRQLIKGNEAIVKAALLAGCRSFYGYPITPASEIAESAAKYFP
                     LAGRTFVQAESETAAINMVYGAAAAGERTMTGSSGPGISLMQEGISYLAGSELPCVIA
                     DIMRGGPGLGNIGPEQSDYNQMVKGGGHGNYHNIVYAPAFAQEMCELTMRAFDVADRF
                     RNPVVVLADGYIGQMMEPVEFPDRGVETSEPDWAVRGDAATRRNLITSIYLDHEKLES
                     HQRKLMAKYARACDELPESRAYRMQGAQIVLVGYGIVGRVLRSVVDLARARGIRAGSI
                     RP"
BASE COUNT          734 a         1580 c         1634 g          733 t
ORIGIN      
        1 ccgcgcccgg atgtcgccgt tcaagcagac ccgcgcgctg gcagaggacg agatcgcccg
       61 gctgttcgac gcggcgcgga ccacgctcga cgagtggacc gaccgcctgc gtcgcgaagc
      121 cggcgatggc ttcccacagc acgtcaccgc gtttcgtcgc gagatggccg tccacggtcg
      181 ctatcgtaga ccgtgccccg actgcggcgc gcccgtgcag cggatcgtct actccggcaa
      241 cgaggccaac tactgcgcgg tctgccagac cggcggcaag ctgctcgccg accgagcact
      301 gtcgcgcctg ctcaagtccg actggccgaa gacgatcgag gagctggagc accggtctcg
      361 atgaccggcg ccgtcccggt cgtcggccgc gcgcgatcag cggcagccga tcgtcgtctc
      421 ggcgatgacc agatcgacca cgcgcttgaa atcgccggtt tcccggtaga ccgacagctg
      481 gcggtcggcg ctcgtcccct cgcgcaagat cgtgtgcgcg tacgcgacct cctcgcgact
      541 gcccaactcc tcgacgacgt cgtcgaccca gtcgagcagc tcgacgatca acgacctgac
      601 cgggagctcc ttgcgcttgc cgaagtcgat cagcagcccg tcgatgccgt gacgcaccgc
      661 gcgccacttg ttctcggtga tcaggttgcg gcggtagtcg cgccagcgga cgttgccgcg
      721 acggagcttg atcagcttgg cgacgatcgc ctggatcaac ccggcgatgc acaccgcctc
      781 ttcgacgcgg gtacagatat cggcgaaacg gaactcgagc gtcgggtagt tcggatgcgg
      841 ccggatgtcc caccagatct tggtcggctc gtcgatgcag ttggtctcga tcagaatcgc
      901 caggaagcgc tggtactcgg cccacgagtt gaacgacggc ggcaatccgg cccgcgggag
      961 attgcccatc acgatcgtgc gatacgactt gagtccggtg tcgcgtccgt gccagaacgg
     1021 cgagctggtc gacaacgcca gcaggtgcgg catgaagtaa cgggcctggt tcatcacgtc
     1081 gatgcgcagc tcgggatcct cgatcccgat gtgcacgtgc atgccgaaga cgagcatctg
     1141 ccgcgcgaca tcgcccatgt cgtgctcgtg tttggtgtag cgctcgccgg cgctgcgctg
     1201 ttgcgagacc cactgcgaga acggatgggt gctggcggcc gcgacgcgcc gacccttggc
     1261 ctccgccatt cggcagacgg tgccgcgcag gcgcagcagc tcggagcgcg cctgttggac
     1321 gtcgctgcag atgcggctgc cgacctcgac ctgcgactgg aggaactcgg gcttgatctg
     1381 cccctcgagc accacgcggc cctgttccag catctcctgg atgtaggagg tcaactgacc
     1441 ggttgccgga tcgatgatct ggtactcttc ttcgattccg atcgtcagcg ggggtgtgga
     1501 catcaggacc gttctccgtc gtcgccggtc ggaggaggaa gcgcgattct aacacggcgc
     1561 cggcagacgg accttggtgc gccggcctcg atcggctagc atccagccga tggtagaggg
     1621 ctcccggact acgccgcgaa cgggccgatc caacaccacg aaactgttgc tcggttgcgc
     1681 ggccggatgc gccgtcgttg ggctcgtgct cgtctcgtcg tgcgtcgggt tcgtctggtg
     1741 gctgaaccgc cccggcgagc tgctcgagcc ggcgcggttg cgcggcagcg acacgaccgc
     1801 ctacgtcgaa tggacgctgc ggttggacga tcccggaacc ggcgctctcg tcgaagacct
     1861 gctcgacgga ttgagcgagc tgcaggagca gatcggcgcg ccgttgccgc ccgggatcgc
     1921 cgacgtgctg aacgagatgc agaatcgcag gaacgaacgg gagttccgcc agctgttccc
     1981 gtgcgtcgtc gcctggagcg tggtcggcga cgacggcggc ggggaagacc tgcacctcgt
     2041 cagcgtgagc atcgagaagc tcggcaaccg catggtgttc atcgattggg tgatggggct
     2101 gttcgtcggc cgcagccccg aggtcgacgt cgtgcgccac ggcggcgaga agatctacgt
     2161 cctgcgcgaa ccggggtcgg cgttcttcct gcgtcggagc gatttcattt tcaccagcga
     2221 cgcggacacc gcgcgtcgcg ccgtggatcg gctcgtggca cagcgggagg agaccgaagc
     2281 gccgtccggg atcgagccgc tgctggccga tctaccgccg gagcacccgc tgcgcggagc
     2341 gtcgttgaat cgcaacgacg agatacgccg cctgttcgaa atggtcaccg gcgacgacga
     2401 gcgcggggtg ctcgacgcgc tcgactggga tcgcgtgaga gcgatgacgt tctccggcgg
     2461 gttcaccgag cagtcgtcgt tcgcggcgct cgtcacgctc gacaccgacg cggccgcctg
     2521 gcacgcggac gagttggccg cgctcgagcg cgtcgtgcgg cgggtcttcg agctcgacgc
     2581 gtcggcgacg caagtcgcgc tcgacagact cgacgaccga atcgtcgtgc ggatcgagct
     2641 cgacgacctg ccgtcgctcg tccggcggcg cctggcgctc gacgggcggt agtcggtcga
     2701 tcagcgggcg gcgacgaacg cggtgaccgc cgagatcgcc tgctcgagcg accgctcctt
     2761 gtccgatccc gaccgcttgc ggggctcgag cgagtggtcg ccatcttcga tgaactcgac
     2821 gcgcacgcgt tccgacagca cataaccggc gatctcctcg cggttgccga gcgggtcgcg
     2881 cgtgccctgg acgatcagcg tgggcgtcgc gatctgcgcc agatgggccg cgcgcaaccg
     2941 ctccggccgt ccggcggggt ggaacgggta accgagacag accagcccgg cgaccccggc
     3001 ctcgtcggcc accaggctcg cgatgcgccc ccccatcgac ttgccgccga tcaccagacg
     3061 gcgagccggt gcgaaccgct cgatcacctc gagccacgag cgacgcagca cgtccccgcg
     3121 gtccggggcg cgtcgcttgc cgtcctcccg acgccgggcc atgtagggga actcgaagcg
     3181 cacgaccgac aggccgcccc ggccgagtcc gtcggcgatc cgattcatga acggcgagtc
     3241 catcggcgct ccggcaccgt gtgccagcac gacccgcgga gagcgagagc cctgcgggcc
     3301 gttggtcagg aatcgctcgt ggtgcatgct gcctccgaag catagcagcg cgcgacgccg
     3361 gccggtcttg gcctacctcc cgcggctgtc gactggggca tccgacgcat ccgcggagcg
     3421 gcatacgccc cgcgaccggc agaacgccgg tggttcgtcg ggacaactga ctcgctgtca
     3481 accggttgcg aggaccggtg cggggcgccg ccgccgcccg ggctggcacg gcgcgtgcag
     3541 ccggttcgag gcgacgccac ccaccgagcg ggtgcgtctc cgaagcggag ggaagcgatg
     3601 gccaaacgga tcgggatcgt cgaggtcgcg agcgactgct gcaaggggtg cggactgtgt
     3661 gtcgcgtctt gtccgacggg gtcgctcgtg ctcggagacg gctggaaccg cctgggctac
     3721 cacccggtcg gattcgtcgt cgacagcggc tgcaccggtt gcggcgtctg cttctacgcc
     3781 tgtccggagc cggcggcgct gcgggtgctg acagccgacc gggccgcgtg agcggtgggc
     3841 gacgccgatg ggcaggcagc tgatcaaggg caacgaggcg atcgtcaagg cggcgttgct
     3901 cgccggctgt cgatcgttct acggctaccc gatcaccccg gccagcgaga tcgccgagtc
     3961 ggccgccaag tacttcccgc tcgccgggcg caccttcgtc caggccgaat cggaaacggc
     4021 ggcgatcaac atggtctacg gggccgcggc ggcgggcgag cgcacgatga ccggctcgtc
     4081 cgggcccggc atttcgctga tgcaggaggg aatctcctac ctcgccggca gcgagcttcc
     4141 gtgcgtgatc gccgacatca tgcgtggcgg ccccggcctg ggcaacatcg gccccgagca
     4201 atcggactac aaccagatgg tcaagggcgg cggccacggc aactaccaca acatcgtcta
     4261 cgcaccggcg ttcgcccagg agatgtgcga gctgacgatg cgcgcattcg acgtcgccga
     4321 ccgattccgc aatccggtcg tcgtgctggc cgacggctac atcgggcaga tgatggagcc
     4381 ggtcgagttc cccgatcgcg gcgtcgagac gagcgagccc gactgggcgg ttcgcggcga
     4441 cgcggccacg cggcggaacc tgatcacgtc gatctacctc gaccacgaga aactggaatc
     4501 gcatcagaga aaactgatgg cgaagtacgc ccgcgcgtgc gacgagctgc ccgagtcccg
     4561 cgcctatcgc atgcagggcg cgcagatcgt gctcgtgggc tacggcatcg tcgggcgcgt
     4621 gctgcggtcg gtcgtcgacc tggcgcgcgc gcgggggatt cgtgccggat cgatccggcc
     4681 g
//
LOCUS       DASPGN010000404         6451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_189969, whole genome shotgun sequence.
ACCESSION   DASPGN010000404 DASPGN010000000
VERSION     DASPGN010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189969"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VD788_11740"
     CDS             <1..653
                     /locus_tag="VD788_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HXV76977.1"
                     /translation="RCGIVFEPHRGWLGKNMYCPACGARMTARPLEVEHALGRRPATT
                     GHGTGAAPRRLLPLVVVVADVRSLWNVGSIFRTADACGVERLFLTGITGHPPRSEITK
                     TALGAEQVVDWEYRPDAAGVLDELARRGYVPVALESGAAAVPLQTVDWPDRVCLVVGN
                     EVAGIAAQCLDACRMQVAIPMLGVKRSFNVAVAFGIAAHRVATVMSERGSLAVGRR"
     gene            650..1699
                     /locus_tag="VD788_11745"
     CDS             650..1699
                     /locus_tag="VD788_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76978.1"
                     /translation="MTSRGRPTVGAGLTAAISATALAAIAAVYADGTIVFPLTIRNVW
                     YRTAAHSGFTGAKHSGDLTIGRQALEFYAKRRSLAIPLEQLWMISYGTMRGDVDTEWA
                     VLTLKDGNERSFVGLRDGSKWGYGQRTREIYETLIDAAVELSAAQFAAPPGFRPYTEL
                     DHIFAMPVPQGWSSYHHELVHVDGAVRWGVVIFSEQELIEEGAPATVSPTSRTTERQR
                     ALRELQQGRATAWILRRDESTGGMSCDGFSERALQTVRQRLADDPFFGVPFEIAADQR
                     FDDGPSIDGCASPRLVLEAQAADGRRLVLDLRVVSRRETYGLIGLRTTADAYTRELAL
                     FEQGVAGFRFAVTRD"
     gene            1722..1970
                     /locus_tag="VD788_11750"
     CDS             1722..1970
                     /locus_tag="VD788_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76979.1"
                     /translation="MAWIETVGEDEAVGLLARMYEQARRRAGKVFHILRIQSLRPRVL
                     RCSTSLYREVMFSRQSGLTRARRELIATVVSRANDCHY"
     gene            2034..2282
                     /locus_tag="VD788_11755"
     CDS             2034..2282
                     /locus_tag="VD788_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76980.1"
                     /translation="MRAAMNDYRTATLADADRAMLDFAVKLTIAPSSIERTDVDALRL
                     VGFDDATIHDIVQVTALFNYYDRLADGLGVDAEPEWDS"
     gene            complement(2225..4060)
                     /locus_tag="VD788_11760"
     CDS             complement(2225..4060)
                     /locus_tag="VD788_11760"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXV76981.1"
                     /translation="MPSPTGERRPKRLGTAALIGLCLLAGGWATAPRDRRAGATAGEP
                     QREFTRLSDEYLSWYFRTHPVRATRLGVHRYDSALPDLSREAIASRIADSRAWLDRLH
                     AIDPAALSRDARFDHRILDHAIRAELLELEEIRGWRHNPLVYSRLIADAFATLVEDRG
                     TTLEQRLPPLTDRVEQLDGLLDAARSNLEHAPGLWIDLAAQGVRGIASFMETELDEQL
                     EAQGLSRVDPGRRLRWDRARRRAVAQLNDFGAWLEGELRASAGGEFRLGRELFERKLW
                     YEEHVALSADELRRLNREQIDRYRLWLTRVARRIGPRDTPEQVMDRITTDHPPPDELL
                     RAAATVIDEIHRFVTDRDLVGLPESELPEVRATPPWARSSFASMSAPGPFEDGGSPAF
                     YNVTNVDPSWTERQQAEHLTYFNFPSLVGIAIHEVMPGHFVQLSWRRRLPTDVRKVFL
                     PASVVEGWAHYVEQMVVDEGLGDEDPAVRLAQLRRALQRHARWDAGLEMHAYGLSIDE
                     AVTRFREIAYFAEFPAQREIQRGTYDPTYLYYALGRMQILALREDYRRYLVRRGRPFS
                     LREFHDRFLELGLPISLAREALMPDDDQLSHSGSASTPNPSAKRS"
     gene            4126..5298
                     /locus_tag="VD788_11765"
     CDS             4126..5298
                     /locus_tag="VD788_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV76982.1"
                     /translation="MNTGRRIIVVGGGVFGLTAAVELCRRGWRVRLFDSQHPPAARAS
                     SSDISKAVRADYGDDTFYGELASLALERWRDWNAASGVELYHEVGFLLLTPRPMEDGG
                     FEYESFRRLRQAGQSPERIDAGVLARRFPAWAGSPYVDGYYNPHAGWSPSARVVEWLA
                     ARATAEGVAIEVGAVDELVVRASRVLGVRTRDGDVHRADRVLVAAGAWTPRLVPAAER
                     ELRAIGQPVLLLKPADPSLFEAARFPVWAADIANTGWYGFPATEAGIVKLANHGPGRP
                     VLPDEPLEVRADEIERFRAFLGASCPALADAPAAGSRLCLYCDTPDGDFWIDHDPTTE
                     GLVVAAGGSGHGFKFAPVLGPIVADVVECRPNRWAARFGWRQPDTGRKEHARSRKE"
     gene            5295..5708
                     /locus_tag="VD788_11770"
     CDS             5295..5708
                     /locus_tag="VD788_11770"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HXV76983.1"
                     /translation="MMYELFEHTADLGLRVRARDLAGLFRDAARGLFAMIVEPEPAGA
                     PVGERSFALSAARYDHLLVDWLGELLCLFDSERLVAGDFRVELDGPKLTASAAIYDVA
                     GGRFHVLREVKAITYHGLKVEPLDGGWLAELIVDI"
     gene            5733..>6451
                     /locus_tag="VD788_11775"
     CDS             5733..>6451
                     /locus_tag="VD788_11775"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HXV76984.1"
                     /translation="MGKRAYDGPLEQVEDYVWRIPKSYKRGMRVDGLIYASERLIEQI
                     RGDQAPEQVANVAFLPGIRLASLAMPDIHWGYGFTIGGVCATDADEGGVISPGGVGYD
                     INCGVRLVRTNLRFDEVEPRLVHLVEALFRRVPAGVGLEGSYRFSARQLRELMAEGVS
                     YLARRGLATDGDIEHTEAGGRLEGARPDFVGERALARGAGQCGTLGSGNHFLEVQVVD
                     RIEQPSVAARLGLEQGGVCVM"
BASE COUNT          961 a         2167 c         2334 g          989 t
ORIGIN      
        1 cacgatgcgg catcgtgttc gagccgcatc gcggttggct cggcaagaac atgtactgcc
       61 cggcgtgcgg tgcgcggatg accgcgcgcc cgctcgaggt cgagcacgcg ctcggccggc
      121 ggccggcaac caccggccac gggaccggcg cggcgccgag gcggctcttg ccgctcgtcg
      181 tcgtcgtcgc cgacgtgcgc agtttgtgga acgtcggttc gatcttccgc accgccgacg
      241 cctgcggcgt cgagcgcctg ttcctgaccg ggatcaccgg ccacccgcca cgcagcgaga
      301 tcacgaagac cgcgctcggt gccgagcagg tcgtcgattg ggagtaccgc cccgacgcgg
      361 cgggcgtgct cgacgagctg gcccgccgcg gatacgtgcc ggtcgcgctc gagagcggag
      421 cggcggccgt cccactgcaa acggtcgact ggcccgatcg cgtctgcctg gtcgtcggca
      481 acgaggttgc cggcatcgcc gcgcagtgcc tcgacgcctg tcgcatgcag gtcgcgatcc
      541 cgatgctcgg cgtcaagcga tcgttcaacg tcgccgtcgc gttcgggatc gccgcgcacc
      601 gcgtcgcaac ggtgatgagc gagcgcggat cgctcgcggt cggccgccga tgacctcccg
      661 cggccgaccg accgtcggcg ccgggctgac cgcagcgatc tcggcgaccg cgctggcggc
      721 gatcgcggcg gtctacgccg acggcacgat cgtcttcccg ctgacgatac gcaacgtctg
      781 gtatcgcacg gcggcacata gcggcttcac cggtgccaag cacagcggcg acctgacgat
      841 cggtcgtcag gcgctcgagt tctacgccaa gcggcgcagt ctggcgattc cgctcgagca
      901 gctgtggatg atctcctacg gcacgatgcg gggcgacgtc gacaccgagt gggcggtgct
      961 gacgctcaag gacggcaacg agcgttcatt cgtcgggttg cgcgacggca gcaaatgggg
     1021 ctacggtcag cgcacacgag agatctacga gacgctgatc gacgccgcgg tggagttgtc
     1081 cgccgcgcag ttcgccgcgc cgcccggctt ccggccgtac accgagctcg accacatctt
     1141 cgccatgccc gtgccgcagg gttggagctc gtaccatcac gagctggttc atgtcgacgg
     1201 ggcggtgcgc tggggcgtcg tcatcttctc ggaacaggag ttgatcgagg aaggggcgcc
     1261 ggcgacggtc tcgccgacct cgcgcaccac cgaacgccag cgcgcgttgc gtgaactgca
     1321 gcagggacgc gcgaccgctt ggatcctacg acgcgacgag tcgaccggtg gcatgagctg
     1381 cgacgggttc agcgagcgcg cgctgcagac ggtccgccaa cggctcgccg atgatccgtt
     1441 cttcggcgta ccgttcgaga tcgcggcgga ccagcgtttc gacgacggac cgtcgatcga
     1501 cggctgtgcg tcgccgcggc tcgtgctcga ggcacaggcg gcggacggcc ggaggctggt
     1561 gctcgacctg cgcgtggtca gccggcgcga gacctacggg ctgatcggtc tgcgaaccac
     1621 cgccgacgcc tacactcgcg aactggcatt gttcgagcag ggggtggccg ggttccgctt
     1681 cgcggtgacc cgagattgac ccgctgcgcg ggaggacgac gatggcctgg atcgagaccg
     1741 tgggtgaaga cgaggcggtc ggactgttgg cgcgaatgta cgagcaagcg cggcggcggg
     1801 cgggcaaggt ctttcacatc ctccgcatcc agtcgctcag gccgcgcgtg ctgcgctgct
     1861 cgacctcgct ctatcgcgag gtgatgtttt cccggcagag cgggctgacc cgcgccaggc
     1921 gtgagctgat cgccacggtc gtctcgcgcg cgaacgactg ccactactga acgcgggcgc
     1981 acggcgagga cctccgggtc gaggtctcgg atcggcagaa cggagaggaa ctggtgcgcg
     2041 cggcgatgaa cgactaccgc acggcgacgc tggccgacgc ggatcgcgca atgctcgact
     2101 tcgcggtgaa gctgacgatc gctccgtcgt cgatcgagcg cacggacgtc gacgccctgc
     2161 gcctggtcgg cttcgacgac gcgacgatcc acgacatcgt ccaggtcacg gcgctgttca
     2221 actattacga ccgcttggcc gacgggttgg gagtcgacgc cgaaccggag tgggacagct
     2281 gatcgtcgtc cggcatcagc gcctcgcgcg cgagtgagat cggcaggccg agctcgagaa
     2341 atcgatcgtg gaactctcga agcgaaaacg gccgcccgcg gcgcacgagg tatcggcggt
     2401 agtcttcgcg tagtgcgagg atctgcatcc gcccgagcgc gtagtacagg taggtcggat
     2461 cgtaggtgcc gcgctggatc tcgcgctggg caggaaactc ggcgaagtac gcgatctcgc
     2521 ggaagcgtgt cacggcctcg tcgatcgaca gtccgtaggc gtgcatctcg agcccggcgt
     2581 cccagcgcgc gtggcgttgc agcgcgcgac gcagctgcgc cagtcgcaca gccggatcct
     2641 cgtcgccgag accctcgtcg accaccatct gctcgacgta atgggcccag ccttcgacca
     2701 ccgacgccgg gaggaagacc ttgcgcacgt cggtcggcag gcgcctgcgc cacgagagct
     2761 ggacgaagtg gccgggcatc acctcgtgga tcgcgatgcc gacgagtgac gggaagttga
     2821 agtaggtcaa gtgctcggcc tgctggcgct cggtccagct cggatcgacg ttggtcacgt
     2881 tgtagaacgc cgggctcccg ccgtcctcga acgggcccgg tgccgacatc gacgcgaacg
     2941 agctgcgcgc ccacgggggc gtggcgcgca cctcgggcag ttcggattcc ggcaggccga
     3001 ccagatcgcg gtcggtgacg aatcgatgaa tctcgtcgat gacggtcgcc gcggcgcgta
     3061 gcagctcatc cgggggcggg tggtcggtcg tgatgcggtc catcacctgc tcgggcgtgt
     3121 cgcgcggtcc gatccgccgt gcgacgcggg tcagccacag gcggtaccgg tcgatctgct
     3181 cgcggttgag ccgacgcagc tcgtcagccg acagcgcgac gtgctcctcg taccacagct
     3241 tgcgctcgaa cagctcccgg cccagtcgga actcgccgcc ggccgacgct cgcagctcgc
     3301 cttcgagcca cgcgccgaag tcgttcagtt gcgcgacggc gcgccggcgc gcacgatccc
     3361 aacgcaaccg ccggccgggg tcgacccgcg acaggccctg ggcctcgagc tgctcgtcga
     3421 gctccgtctc catgaacgaa gcgatgccgc gcaccccctg cgccgccagg tcgatccaca
     3481 acccgggcgc gtgctcgagg ttcgaccgcg ccgcgtcgag cagaccgtcg agctgctcga
     3541 ctctgtcggt cagcggcggc aagcgctgct cgagcgtggt accacggtcc tcgaccagcg
     3601 tggcgaacgc gtcggcgatc aaccggctgt agaccagcgg attgtggcgc cagccgcgga
     3661 tctcttccag ctcgagcaat tcggctcgga tcgcgtggtc gaggatgcga tggtcgaacc
     3721 gggcatcgcg cgacagcgcc gccgggtcga tcgcatgcag ccggtcgagc caggcacggg
     3781 agtcggcgat tcggctcgcg atcgcttcgc gactgagatc gggcagcgcc gagtcgtacc
     3841 gatggacccc gagccgggtc gcccgcacgg gatgcgtgcg gaagtaccac gacaggtact
     3901 cgtccgacag gcgggtgaat tcccgttgtg gctcgccggc cgtcgcgccg gcccggcggt
     3961 cacgcggagc ggtcgcccat ccgccggcga gaaggcagag tccgatcaac gccgccgtcc
     4021 ccagccgctt cggccggcgc tcgcctgtcg ggctgggcat cacgctcacc cagtcgaatc
     4081 tatactgggt cgcggaagag gcaacccgcg gcgccggagg ggaccgtgaa caccgggcga
     4141 cggatcatcg tggtcggagg aggggtgttc ggactgacgg cggcggtcga gctgtgccgc
     4201 cgcggctggc gagttcggct gttcgactcg cagcatccgc ccgcggcgcg cgcatcatcg
     4261 agcgacatca gcaaggccgt gcgcgcggac tacggcgacg acacgttcta cggcgagctg
     4321 gccagcctgg cgctcgagcg ctggcgcgac tggaacgcgg cgtcgggcgt cgagctgtac
     4381 cacgaggtcg gcttcctgct gctgacgccg cgtccgatgg aggacggcgg cttcgagtac
     4441 gaatcgttcc gccgcctgcg ccaggccggt cagtcccccg agcgcatcga cgcgggtgtg
     4501 ctcgctcgac gctttcccgc ctgggccggg tcgccctacg tcgacggcta ctacaaccca
     4561 cacgcgggat ggtctccgag cgcgcgcgtc gtcgagtggt tggccgcgcg cgcgaccgcc
     4621 gagggagtcg cgatcgaggt cggtgccgtc gacgagttgg tcgtccgcgc ctcgcgggtg
     4681 ctcggcgtgc gcacgcgcga cggcgacgtc caccgcgcgg accgcgtgct cgtcgccgcg
     4741 ggtgcctgga ccccgcgtct ggttccggcg gccgagcgcg agctgcgggc gatcgggcaa
     4801 ccggtactcc tgttgaagcc ggccgatccg tcgctgttcg aagcggcgcg ttttccggtg
     4861 tgggcggccg atatcgcgaa caccggctgg tacggattcc cggcgacgga ggcggggatc
     4921 gtcaagctgg cgaaccacgg accgggccga ccggtcctgc cggacgagcc gctcgaggtg
     4981 cgcgccgacg agatcgagcg ctttcgcgcc ttcctcggcg ccagttgccc ggcgttggcc
     5041 gacgccccgg cggccggcag ccgtctctgc ctgtattgcg acacgcccga cggcgacttc
     5101 tggatcgacc acgatccgac gaccgagggt ctggtcgtcg cggccggcgg cagcgggcac
     5161 ggcttcaagt tcgcgccggt gctcggcccg atcgtcgcgg acgtcgtcga gtgccgtccc
     5221 aaccgctggg ccgcgcgttt cggctggcgc cagcccgaca ccgggcgcaa ggaacacgct
     5281 cgaagtcgga aggaatgatg tacgagctgt tcgagcacac ggccgacctc gggttgcggg
     5341 tcagggcccg cgacctcgcc ggcctgttcc gcgatgcggc gcgaggtctg ttcgcgatga
     5401 tcgtcgagcc cgagcccgcg ggagctccgg tcggcgagcg gagcttcgcg ctgtcggccg
     5461 cgcgttacga tcatctgctg gtcgactggc tcggcgagtt gctctgcctg ttcgacagcg
     5521 agcgactcgt cgcgggcgat tttcgcgtcg agctcgacgg gccgaaactg acggccagcg
     5581 ccgcgatcta cgatgtcgca ggcggccggt tccacgtgct gcgcgaagtg aaggcgatca
     5641 cctatcacgg attgaaggtc gaaccgctcg acggcggatg gctcgcggag ctgatcgtcg
     5701 acatctgagc ggagttcgac gttgggaagg cgatgggcaa acgagcatac gacggaccgc
     5761 tcgagcaggt cgaggactac gtttggcgca tccccaagag ctacaagcgc ggcatgcggg
     5821 tcgacgggtt gatctacgct agcgagcggc tgatcgagca gatccgcggg gatcaagcac
     5881 cggagcaggt ggcgaatgtc gcgttcctgc ccgggatccg tctggccagt ctggcgatgc
     5941 cggacatcca ctggggctac ggcttcacga tcggcggggt ctgcgcgacc gacgccgacg
     6001 agggcggcgt gatctcgccc ggcggggtgg gctacgacat caactgcgga gtgcgtctcg
     6061 tccgcaccaa cctgcggttc gacgaagtcg agccgcgtct cgtgcacctc gtcgaggcgc
     6121 tgttccgccg cgtgccggcc ggcgtcgggc tcgaggggag ctaccgcttc agcgcccggc
     6181 aactgcgcga gttgatggcg gaaggcgtga gctatctggc ccggcgcggc ctggcgaccg
     6241 acggcgacat cgagcacacc gaggccggtg gccggctgga gggcgcccgc cccgacttcg
     6301 tcggcgagcg ggcccttgcc cgcggcgccg ggcagtgcgg cacgctcggc tcgggcaacc
     6361 actttctcga agtccaggtc gtcgatcgga tcgaacagcc gagcgtcgcc gcgcggctgg
     6421 gtctggagca gggaggagtc tgcgtgatga t
//
LOCUS       DASPGN010000405         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1477587, whole genome shotgun sequence.
ACCESSION   DASPGN010000405 DASPGN010000000
VERSION     DASPGN010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477587"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1713
                     /locus_tag="VD788_11780"
     CDS             <1..1713
                     /locus_tag="VD788_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV76985.1"
                     /translation="GLTQADAGRVVLRGKDVTGDAPQRRGVGMVFQQYALFGHMSVAD
                     NVEFPLKIRRVDARERARRRDQLLELVGLVGYQRRFPIQLSGGQQQRVALARALAHNP
                     QVLLLDEPFGALDARIRTELRRNLQEIRRELRVTTIFVTHDQEEAVELADRLGVMNFG
                     RLLEVGTADELYLRPKTEFVATFLGKANLFVGECSADEVKLGAARFPLETRSNADPPQ
                     RRVQVLFRPEDVAVKLTRDALDWPLLGEAVVENVEFAGSSERLRLRMPAEPGVRAIAP
                     AVPFGADYLLVDATRSQHLARRYPLAVGDRAWIGVRRLHALAHRGLSFAVVDRGRFVT
                     SGAIEYAGTIARLCHARVSIVVSNGHGAAGVDRAQAARERLGSGLASVTAHGVESESR
                     AFVRELDRIRPDLVVVPRVDLDERFGLEDLLAADLRSVLVARAGASAPRRVILCTAAG
                     DAAKADIVVAGRILRHFGSPVTILTVVQPGDPVDREQLVRFHDLSRRTLQLHGLDAVS
                     EIVDGEPREWIARRARDEGHDLVVLGAPPAEADARTRFTRQAIELTDQVDIPLLIVNA
                     GEAA"
     gene            1713..>3011
                     /locus_tag="VD788_11785"
     CDS             1713..>3011
                     /locus_tag="VD788_11785"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HXV76986.1"
                     /translation="MRRGVWPAAILIAVTSLAVSPAAEEERPTKRLTFGGSVRARLSR
                     LWNQFRPGLDENDQAFSLRTLLRTEVTLPRLSLVAEFQDSRAYLTDENSAVSTIVVNA
                     AGLLQGYVGTRLNDVFTEGATLDLRLGRQTIDLGGRRLVARNRFRNTIQNYSGLTAHW
                     RGNDQSDWFAFFVLPVLVRPDNLDRAGLLDNDIASDEEDFDLRFWGASYRRPLGRGSI
                     GELYLFGLEEDDDPGELETRNRRLYTPGLRWLRPPGARRWDFDVETTVQFGTRRATPL
                     PADVEPLDVRALFQHGTIGYTFDEPWSPRLGAEWDYATGEADSSDGEWGRFDTLFGPR
                     RTEFGPTGIYGILGRENIVSVGPRLTLSPGSRLDGFVSWRANWLDERSDFFARSLVRD
                     PSGESGGFAGHQLELRGRYWWVPSTLCWEIGAALFLEGRFM"
BASE COUNT          425 a         1040 c         1077 g          469 t
ORIGIN      
        1 ggcctgacac aggccgacgc cggtcgcgtc gtattgcgtg ggaaggacgt caccggcgac
       61 gcaccgcagc gccgcggcgt cggcatggtc ttccagcagt acgcgctgtt cggccacatg
      121 agcgttgccg acaacgtcga gttcccgctc aagatccgcc gcgtcgacgc tcgcgaacgc
      181 gcgcgacggc gggatcagct cttggagctg gtcggtctcg tcggctacca gcgacgcttt
      241 ccgattcagc tgtcgggggg tcagcagcag cgagtcgcgc tcgcccgggc gctcgcacac
      301 aacccacagg tgctgttgct cgacgagccg ttcggtgcgc tcgacgcgcg gatccggacc
      361 gagttgcgcc gcaacctgca ggagatccgg cgcgagctga gggtgacgac gatcttcgtg
      421 acccacgacc aggaagaggc ggtcgagctc gccgaccggt tgggtgtgat gaacttcggc
      481 cgcttgctcg aggtcggtac cgccgacgag ctctacctgc gcccgaagac cgagttcgtc
      541 gccacctttc tcggcaaggc caacctgttc gtcggcgaat gcagcgccga cgaggtcaag
      601 ctcggcgcgg cgcgcttccc gctcgagacg cgctcgaacg ccgatccgcc gcaacgccgg
      661 gtgcaggtcc tgtttcgtcc cgaagacgtc gcggtcaagc tgacgcggga cgcgttggac
      721 tggccgttgc tcggagaggc cgtcgtcgag aacgtcgagt tcgccggctc gagcgaacgg
      781 ctgcggctgc ggatgccggc cgaaccgggc gtgcgggcga tcgcacccgc ggtcccgttc
      841 ggggccgact acctgctggt cgacgcgacc cggtcccaac acctcgcgcg gcgctacccg
      901 ttggccgtcg gcgacagggc ctggatcggc gtgcgacgcc tgcacgcgct ggcgcaccgg
      961 gggctgagct tcgcggtcgt cgatcggggt cggttcgtca cgtcgggagc gatcgagtac
     1021 gccgggacga tcgcgaggct gtgccacgcg cgggtgagca tcgtcgtgtc gaacggacac
     1081 ggggcggccg gcgtcgatcg cgcccaggcc gcgcgagagc gattgggaag cgggttggcc
     1141 tcggtcaccg cgcatggcgt cgagtcggag tcgagggcct tcgtgcgtga gctcgaccgg
     1201 atccgcccgg acctggtcgt cgtgccccgc gtcgacttgg acgagcgctt cggcctcgag
     1261 gacctgctgg cggccgatct ccgcagcgtg ctggtcgcac gagccggcgc gagcgcgccg
     1321 cggagggtga tcctgtgcac cgcggcgggc gatgcggcga aggcggacat cgtcgtcgcc
     1381 gggcggatcc tgcggcactt cggctcgccg gtgacgatcc tgaccgtcgt ccagcccggc
     1441 gacccggtcg accgggagca gctcgtccga ttccacgacc tgtcgaggcg caccctccag
     1501 ctccacggcc tcgacgccgt ctccgagatc gtcgacggcg agccacggga gtggatcgca
     1561 cggcgcgcga gggacgaggg gcacgatctc gtcgtgctcg gggcgcctcc agccgaggcc
     1621 gacgcccgca cgcggttcac ccggcaggcg atcgagctca ccgatcaggt cgacatcccg
     1681 ctgctgatcg tcaacgcggg cgaggccgcg tgatgcggcg cggcgtgtgg ccggcggcga
     1741 tcctgatcgc cgtcacctcg ctggcggtct caccggcggc ggaggaggag cgcccgacca
     1801 agcggctgac cttcggcggc agtgtccgag cgcgcctgtc gcggctgtgg aatcagttcc
     1861 ggcccggtct cgacgagaac gaccaggcgt tcagcttgcg cacgttgctg cggacggaag
     1921 tcacgctccc gcggttgtcg ctcgtcgccg agttccagga ctcgcgcgcc tacctcaccg
     1981 acgagaacag tgcggtgtcg acgatcgtgg tcaacgccgc gggtctgctc caggggtacg
     2041 tcgggacccg gctcaacgac gtcttcaccg agggagcgac gctcgacctg cgcctcgggc
     2101 gtcagacgat cgacctcggc ggaaggcggc tcgtcgcgcg caatcgcttc cgcaacacga
     2161 tccagaacta cagcgggctg accgcgcatt ggcgcggcaa cgaccaatcc gattggtttg
     2221 ccttcttcgt gctgccggtg ctcgttcgac ccgacaacct cgaccgagcc ggcctgctcg
     2281 acaacgacat cgcgtccgac gaggaggact tcgacttgcg cttctggggg gcgtcgtacc
     2341 ggcgtcccct cggccgggga tcgatcggcg agctctacct gttcggtctc gaggaggacg
     2401 acgatcccgg cgagctcgag acgaggaatc gccggctcta cacgcccggc ctgcgctggc
     2461 tgcgaccgcc cggcgctcgc cggtgggatt tcgacgtcga gacgacggtg cagttcggca
     2521 cgagacgcgc cacgcccctg ccggccgacg tcgaaccgct cgacgtgcgg gcgctgttcc
     2581 agcacgggac gatcggctac acgttcgacg aaccgtggtc gccgcggctc ggcgccgaat
     2641 gggactacgc gaccggcgag gccgactcga gcgacggcga atggggccgc ttcgacacgc
     2701 tgttcgggcc gcggcgaacg gagttcggcc cgaccggcat ctacgggatc ctcggccgcg
     2761 agaacatcgt ctcggtcgga ccgcgcctga cgctgagtcc cggctcccgg ctcgacggat
     2821 tcgtctcgtg gcgcgccaac tggctcgacg agcgctcgga cttcttcgcc cgctcgctcg
     2881 tccgcgaccc gtcgggcgag tccggtggtt tcgccggaca tcagctcgaa ctgcgcggcc
     2941 gctactggtg ggtgccgtcc accctgtgtt gggagatcgg cgcggcgctc ttcctcgaag
     3001 gccggttcat g
//
LOCUS       DASPGN010000406         7010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_658811, whole genome shotgun sequence.
ACCESSION   DASPGN010000406 DASPGN010000000
VERSION     DASPGN010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7010
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658811"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VD788_11790"
     CDS             <1..624
                     /locus_tag="VD788_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXV76987.1"
                     /translation="LARAVHAVEALDGDRLERELSEAAVDLPTPQLLERVLAPLLRSI
                     GDGWREGRIRPSHEHLTTAVVRSFLGVIALRRDSAGTGGELVVATPAGQLHELGALMA
                     AVAAVLDGWRVTYLGPNLAADEIALAARQRGARAVALSVIYAGDPNRIEAELRELRRL
                     IGADIGLFVGGAGLAAVTGALAEVGALRIDSIDSFRRVLERLRGKTT"
     gene            696..1769
                     /locus_tag="VD788_11795"
     CDS             696..1769
                     /locus_tag="VD788_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76988.1"
                     /translation="MSVYRSIAELPVRIDAVEFEGLVRPAQRGGFGRRTTIVRLRGAG
                     ATGVGEDVNYDAGEQKELVDHGLDLPIRGNRRLGRLERLLDELDLFGREPRHPEARAY
                     RRWAVSSAALDLALRQQRRPFHHLVGLVPRPVRFVKSMSLGAAASIEPVSRILRRRPT
                     TRFKLDAGRGWTAATADSLRGTGAVEIVDLKGAYHGTPVDLAADPALYLTIARGLPDV
                     WLEDPAWNRETRAVLEPFLDRVTWDAPIHSAAEIEALAPRPRMVNMKPSRFGSLARLF
                     EAYAWCRANGVGMYGGGQFELGPGRGQIQYLAALFHPEAPNDVAPTAYHDAETATDLP
                     PSPLRPAPAAAGFAWREPQPESR"
     gene            1769..2908
                     /locus_tag="VD788_11800"
     CDS             1769..2908
                     /locus_tag="VD788_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76989.1"
                     /translation="MSAELTGDALERLVRTVFRPRSTDRRLALMFDLPDTVRPDHDEW
                     ADRRELVADWARKLARAGARLGLDSVDRVAYRNVRNNNADLPPEAWVVSGAEVPDDAD
                     RIASPATPMERILDGYELLIAVTELSATAPLKLAARRFGFRAATMPGFTRGMIPTLGL
                     DWDEIHQRCVALAGRLSRATAAEVRLTAAGRACRLTIDLRHRSAAASGGLIREPGTVS
                     NLPSGESYIVPYEGEIEGDPSRTSGVLPVRLDGELLFYRVEANRAVGVEGDGPVASRE
                     AAKVREEPAYANIAELGLGVLSEYGIRPVGRTLLDEKLALHIAFGRSDHFGGRVGAAQ
                     FSSPEQVVHVDRVYVPEMQPGIAVVGVDLIDDDGGEALIRDGRYV"
     gene            complement(2922..3893)
                     /locus_tag="VD788_11805"
     CDS             complement(2922..3893)
                     /locus_tag="VD788_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76990.1"
                     /translation="MHDDGRGRDLSARLTVFGAGAVLIGCGLVALALLHLALPRITRG
                     LDAGGTAVDPATAWMGAALYSLLGLALVGSGVGSIRKRRWVRPIMLFISGTWLMVGSF
                     VTGLVVLVADDLVALAAGDLIVSTPELVGPVRALMIGLSAGFGLVLPALFFWAYRDRD
                     VETTCARHSPGAGWSDRCPPAVLALSAALAGAGLIGLPSVFRPVFPLFGRVATGWPAV
                     VGLLMVSAWCLWLGWSTFRLEREGWWGTLVFFTVLGWSTVVTALAVDPLEFYLLLGYD
                     EEQLELLRPSAGAARWLMVGSSVALTVLGVVYLLGVRRHFSGRRRTG"
     gene            complement(3973..4749)
                     /locus_tag="VD788_11810"
     CDS             complement(3973..4749)
                     /locus_tag="VD788_11810"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXV76991.1"
                     /translation="MLRPKMILAVLLLFAVTAIVVTPLLGQGGEAKNLEERVVALEER
                     IATLERTMAQRLASLERRVEAGAAGPVDDPAAEAAYAEISRLMASGEYEKAKTEMASF
                     MKQYGETNTAKKARRTYQELSVIGKASPETWDIEKWFQGENQVDLNKGTTLLVFWEEW
                     CPHCKREVPKLQSWYTDLKGQGLQIIGLTKLTRSSTEEKVTSFIADNHVDYPMAKETG
                     ELSRYFNVSGIPAAAVIKDGKVVWRGHPAQLNEAQLKSWL"
     gene            4904..5410
                     /locus_tag="VD788_11815"
     CDS             4904..5410
                     /locus_tag="VD788_11815"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HXV76992.1"
                     /translation="MSRSRGPTVLTPRRFRLLLRLFPPFLAGRTRVVDVSDDFLRCRV
                     RVRRSPLTRNLQGSIFGGTIFSAADPFFAMLYWQALARRGLRVQAWIRSARIVYLKPA
                     ESALTFEFEVAENEVRQALEALEREGRFARLHTTRAVDEQGQVCAVVELEVYLRRPRD
                     DRREAAAF"
     gene            complement(5425..5814)
                     /gene="apaG"
                     /locus_tag="VD788_11820"
     CDS             complement(5425..5814)
                     /gene="apaG"
                     /locus_tag="VD788_11820"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HXV76993.1"
                     /translation="MPESDAITRGVRVHVVSRHDPDRSDPENGQWFFLYTIRITNEGD
                     RTVRLLSRHWIITDSSGKVEEVRGPGVVGHQPTLAPGESFEYTSGCPLETAFGTMHGS
                     YEMVVEGGGAFRAEIAPFALGLPYSIN"
     gene            5979..6983
                     /locus_tag="VD788_11825"
     CDS             5979..6983
                     /locus_tag="VD788_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76994.1"
                     /translation="MNYLTQDKLAIFGSAGAIGSNMVQSALTMGITPNIAMYDPFATG
                     NEGAAEEIYHCAFPGARVRWTSDPAEALDGASFVISAGGAPRKEGMTREDLLKGNAEI
                     AVQLGREIKRQAPGLKFAVIIFNPADITGLVTLVHAGLPASRVATLAALDSTRLQTAL
                     AQHFDVAQDEVTGARTYGGHGEMMAVFASTVRISGRPLTELIGTPRLSDQAWSEIQTR
                     VRQGGKRIIQLRGRSSFQSPAHQSVLMVKAVIDGKGYPWPCGTYVDSAKHGMAKVMMA
                     METRLDRDGVYWELPRGTEDELAALRASYEHVAKLRDEVVAMGILPPLDRWSAINPRL
                     "
BASE COUNT         1091 a         2432 c         2463 g         1024 t
ORIGIN      
        1 ctggcccgcg cggtgcacgc cgtcgaagcg ctcgacggcg atcggctgga gcgggagctg
       61 tcggaggctg ccgtcgatct gccgaccccg cagctgctcg agcgggtgct ggcgccgctg
      121 ctgcggtcga tcggcgacgg ctggcgcgag ggccgaatcc gcccgagtca cgagcacctg
      181 accacggccg tcgtccgctc gttcctcggg gtgatcgcgc tgagacggga ttcggccggg
      241 acgggcggcg agctcgtggt cgcgacaccg gcgggacagc tgcacgagct gggagcgctg
      301 atggcggcgg tcgcggcggt gctcgacggc tggcgcgtga cctacttggg cccgaacctc
      361 gcggccgacg agatcgcgtt ggccgcccgg cagcgcggcg cccgcgcggt cgccctcagc
      421 gtgatctacg ccggcgaccc gaatcggatc gaggccgagc tacgcgagct gcggcggctg
      481 atcggcgccg acatcgggtt gttcgtcggc ggggcgggtc tcgccgccgt gacgggggcg
      541 ttggccgagg tcggtgcgct gcgcatcgat tcgatcgact ccttccgccg cgtgctcgag
      601 cgcttgcgcg ggaagacgac ctagccgacg ggcgacccgc gcttcggcgc cgcaaacggt
      661 cgttccgttc ggtccgggcc gttacaatgg ccgggatgtc cgtctaccgg tccatcgccg
      721 agctgccggt gcggatcgac gccgtcgagt tcgaggggct ggtccgaccg gcccagcggg
      781 gcggcttcgg ccgccgtacc acgatcgtgc gtctccgcgg agccggcgcg acgggggtcg
      841 gcgaggacgt caactacgac gccggcgagc agaaggagct ggtcgaccac gggctcgacc
      901 tgccgatccg cggcaaccgc cggctggggc gtctcgagcg gttgctcgac gagctcgatc
      961 tgttcgggcg ggagccgcgc catccggaag cccgcgccta ccgccgctgg gccgtctcga
     1021 gcgccgcgct cgatctggcg ctgcgccagc aacgccgccc gttccaccac ctcgtcggcc
     1081 tcgtcccgcg accggtccgg ttcgtcaaat cgatgtcgct cggcgccgcc gcgtcgatcg
     1141 agccggtgtc gagaatccta cgccgccggc ccacgacccg attcaagctc gatgcgggtc
     1201 gaggctggac cgcggcgacc gccgacagcc tgcgtggcac cggcgcggtc gagatcgtcg
     1261 acctcaaggg cgcctaccac gggacccccg tcgatctcgc cgccgatccc gcgctgtacc
     1321 tcaccatcgc ccgcggcctg ccggacgtct ggctcgaaga tccggcatgg aaccgcgaaa
     1381 cgcgtgcggt gctcgaaccg ttcctcgatc gtgtcacctg ggacgcgccg atccactccg
     1441 cggccgagat cgaggcgctc gcgcctcggc cgagaatggt caacatgaaa ccgtcgcgct
     1501 tcggctcgct ggctcgcctg ttcgaggcct acgcttggtg ccgcgcgaac ggggtcggca
     1561 tgtacggcgg tggacagttc gagctcggtc cgggtcgggg tcagatccag tatctcgccg
     1621 cgctgttcca cccggaagcg ccaaacgacg tcgcaccgac cgcctaccac gatgccgaaa
     1681 cggcgaccga tctgccgccg tcgccgctgc ggccggcgcc cgcggccgcc ggcttcgctt
     1741 ggcgcgagcc gcaaccggag agccggtgat gagcgccgag ctgacgggcg acgcgctcga
     1801 acggctggtg cggaccgtct tccgcccgcg gtcgacggac cggcgattgg cgctgatgtt
     1861 cgatctgccg gacacggtcc gacccgacca cgacgagtgg gccgatcggc gggagctggt
     1921 cgccgactgg gcgcgaaagc tggcccgggc cggcgcgcgg ctcgggctcg actcggtcga
     1981 tcgggtggcc taccgcaacg tgcgtaacaa caacgcggac ctcccgccgg aggcgtgggt
     2041 cgtgagcggt gccgaggttc ccgacgacgc agaccgcatc gcgtcgccgg cgacgccgat
     2101 ggagcgcatc ctcgacggct acgagctgct gatcgccgtc accgagctgt cggcgacggc
     2161 accgctcaag ctcgccgcgc ggcgtttcgg ctttcgcgcc gcgacgatgc cgggctttac
     2221 gcgcggcatg atcccgacgc tcggactgga ctgggacgag attcatcaac ggtgcgtggc
     2281 gctcgccggg cggctgagcc gcgcgaccgc cgccgaggtg cggttgaccg cggcgggacg
     2341 tgcgtgtcgg ttgacgatcg acctccgaca tcgcagcgcc gcggccagtg gaggcctgat
     2401 tcgcgagccc ggtacggtga gcaacctgcc gtcgggcgag agctacatcg tgccgtacga
     2461 aggggagatc gagggcgatc ccagccgtac ctcgggcgtg ttgccggtgc gcctcgacgg
     2521 cgagctgctg ttctatcgcg tcgaggccaa ccgcgcggtc ggcgtcgagg gcgacggacc
     2581 ggtcgcgagc cgcgaagcgg cgaaggtgcg ggaggaaccg gcctacgcca acatcgcgga
     2641 gctggggctg ggcgtgctga gcgagtacgg catccggccg gtcggccgga ccttgctcga
     2701 cgagaagctg gcattgcaca tcgccttcgg tcggagcgat cacttcggcg gtcgtgtcgg
     2761 cgcggcgcag ttcagctcgc ccgagcaggt cgtccacgtc gaccgcgtct acgtgccgga
     2821 gatgcagccg gggatcgcgg tggtgggcgt cgatctgatc gacgacgacg gtggcgaggc
     2881 gttgatccgg gacggcaggt acgtctgacg acgggtcgcg atcagccggt gcgccgacgt
     2941 ccggagaagt gacgccgcac gccgagcagg tagaccactc ccagcacggt cagcgccacc
     3001 gagctaccga ccatcaacca acgtgcggcg ccggccgagg gccggagcaa ctcgagctgc
     3061 tcctcgtcgt agccgagcag caggtagaac tcgagcgggt cgaccgcgag cgcggtgacg
     3121 accgtcgacc aaccgagaac cgtgaagaac acgagcgttc cccaccaacc ctcacgctcc
     3181 agacggaagg tcgaccagcc gagccacaga caccaggccg acaccatcag caggccgacg
     3241 acggcgggcc agccggtcgc cacccggccg aacagcggga acaccggccg gaagaccgac
     3301 ggcagcccga tcaagcccgc gccggccagc gccgcgctca gcgcgagcac cgcgggcggg
     3361 cagcgatcgc tccagcccgc gccgggcgag tggcgggcgc aggtggtctc gacgtcgcgg
     3421 tcgcgatagg cccagaagaa cagcgcgggc aggaccaggc cgaacccggc gctgaggccg
     3481 atcatcagcg cgcgaaccgg cccgaccagc tcgggtgtcg agacgatcag gtcaccggcg
     3541 gccaacgcga cgagatcgtc ggccaccaac accacgagcc cggtgacgaa cgacccgacc
     3601 atcagccacg tgccgctgat gaacaacatg atcggacgaa cccagcgacg cttgcgaatc
     3661 gagccgacgc cggacccgac cagcgccaga cccagcagcg agtagagcgc ggcgcccatc
     3721 cacgcggtcg ccggatcgac cgccgttccg ccggcatcga gtccacgcgt gatccgcggc
     3781 agggccaggt ggagcaacgc gagcgcgacg agcccgcatc cgatcagtac cgcaccggcg
     3841 ccgaagacgg tcaggcgagc actgagatcg cgccctcgcc cgtcgtcatg catgcggcca
     3901 gtataagaaa aaacggcgcg ggtgcgaccc gcgccgtttc gtgacgagct ccgcgagcga
     3961 tcggtcggcc ggtcagagcc aggatttcaa ctgggcctcg ttgagctgtg ccggatggcc
     4021 gcgccagacg accttgccgt ctttgatgac cgcggcggcc gggatcccgc tgacgttgaa
     4081 gtaacggctc agctcaccgg tctccttggc catcgggtag tcgacgtggt tgtccgcgat
     4141 gaacgaggtc accttttctt cggtcgacga gcgggtgagc ttggtcaagc cgatgatctg
     4201 caggccctgg cccttgaggt ccgtgtacca cgactgcagc ttcggtacct cgcgcttgca
     4261 gtgcggacac cactcttccc agaagacgag caacgtcgtt cccttgttca ggtcgacttg
     4321 gttctcgccc tggaaccact tttcgatgtc ccacgtctcc gggctggcct tgccgataac
     4381 ggacagctct tgataggtgc gacgcgcctt cttcgcggtg ttggtctcgc cgtactgctt
     4441 catgaacgag gccatctcgg tcttggcctt ctcgtattcg cccgaggcca tcagccgcga
     4501 gatctccgcg taggccgctt cggccgccgg atcgtccacc ggccctgccg cgccggcctc
     4561 gacccgccgc tcgagcgagg cgagacgctg ggccatcgtg cgttcgagcg tggcgatccg
     4621 ttcctcgagg gcaacgaccc gctcctccag gttcttggcc tcgccaccct ggccgagcag
     4681 cggcgtgacc acgatcgccg tgacggcgaa cagcaagagc acggcgagga tcatctttgg
     4741 tcggagcatg gtgcctccct cgatcggttc ccgggttttc gaactctcgc ggtcccttca
     4801 atatacagtg cccccccgcc gagcgtccat cgaccggcgg ccgggcctgg cgacgcggtt
     4861 ccgcccccgg tatgatcgcg cccgacgagc cgagggaggc gtcgtgtcca ggtctcgcgg
     4921 tccgaccgtc ctgaccccgc gccgattccg gctgttgctg aggctgtttc cgccgtttct
     4981 cgccggccgc acgcgggtgg tcgacgtgtc ggacgacttc ctccgctgcc gcgtccgtgt
     5041 ccggcgcagc ccgttgaccc gcaacctgca ggggtcgatc ttcggtggca cgatcttctc
     5101 cgcggccgat ccgttcttcg ccatgctgta ctggcaggcg ctcgcccgac gcgggctgcg
     5161 cgtgcaggcc tggatccggt ccgcccgcat cgtctacctc aagcccgccg agagtgcgct
     5221 gaccttcgag ttcgaggtcg ccgagaacga ggtccggcag gcgctcgagg cgctcgagcg
     5281 cgaaggacgc ttcgcgcgcc tccacacgac gcgcgccgtc gacgagcagg ggcaggtctg
     5341 cgccgtggtc gagctcgagg tctacttgcg tcgcccgcgg gacgaccggc gcgaagcggc
     5401 ggcgttctga ccggcggcga ctcgtcagtt gatcgagtac gggagaccca acgcgaatgg
     5461 ggcgatctcc gcacgaaacg ccccaccccc ctcgaccacc atctcgtagc tgccgtgcat
     5521 cgtgccgaac gcggtctcga gcggacaccc cgaggtgtac tcgaacgact cgccgggggc
     5581 gagcgtcggc tggtggccga ccaccccggg tccgcggact tcctcgacct tgccgctcga
     5641 gtcggtgatg atccaatgcc gactcagcag ccggaccgtc cggtcgccct cgttcgtgat
     5701 acggatcgtg tagagaaaga accactggcc gttctcggga tcggagcggt cggggtcgtg
     5761 gcgggatacg acgtggacgc gcacgccgcg ggtgatcgcg tcggattcgg gcatcgtttc
     5821 gcgcgagatt ctagcacgcg cgcccgaggg gaaaaacgcc cgggcgagtc ccgacgaaaa
     5881 cggtcgaatc acgacgcggg cgacgcaaaa aatgctagaa ctagggggga atccgcgagg
     5941 gacgtcacgc cgcacctcgc tcccaggggg atgactcgat gaactacctg acgcaggaca
     6001 agctcgcgat cttcggatcg gcgggcgcga tcggctcgaa catggtgcaa tcggcgctga
     6061 cgatgggaat cacgcccaac atcgcgatgt acgatccatt cgccacgggg aacgagggcg
     6121 ccgccgagga gatctaccac tgcgcgtttc ccggtgcccg tgtccgctgg acgtcggacc
     6181 ccgccgaagc actcgacggc gcgtcgttcg tcatctccgc cggcggcgcg ccgcgcaagg
     6241 aggggatgac ccgcgaggac ctgctcaagg gcaacgcgga gatcgccgtc cagctgggtc
     6301 gcgagatcaa gcggcaggcg cccgggttga agttcgccgt gatcatcttc aaccccgccg
     6361 acatcaccgg actcgtcacc ctcgtgcacg cggggctccc cgcgtcgcgc gtcgcgacgt
     6421 tggccgcgct cgacagcacg cgcctgcaga ccgcgctggc ccagcacttt gacgtcgcgc
     6481 aggacgaggt gaccggcgcg cggacctacg gcgggcatgg cgagatgatg gcggtcttcg
     6541 ccagcacggt ccgcatctcc ggccggccgt tgaccgaact gatcggcacg ccgagactga
     6601 gcgaccaggc gtggagcgag atccagacgc gcgtgcgcca aggcggcaag cgcatcatcc
     6661 agctgcgcgg tcgcagctcg ttccagagcc ccgcgcacca gtcggtgctg atggtcaagg
     6721 cggtgatcga cggcaaggga tacccgtggc cgtgcggcac ctacgtcgac tcggcgaagc
     6781 acgggatggc caaggtgatg atggcgatgg agacgcgact cgaccgcgac ggggtctact
     6841 gggagctgcc gcgaggcacc gaggacgagc tggccgcgct gcgcgcctcg tacgagcacg
     6901 tcgccaagct gcgcgacgag gtggtcgcga tgggcatcct gccgccgctc gaccgctggt
     6961 cggcgatcaa cccccggctg tagcgcggca cggagcgggc gatgggcaaa
//
LOCUS       DASPGN010000407         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1009263, whole genome shotgun sequence.
ACCESSION   DASPGN010000407 DASPGN010000000
VERSION     DASPGN010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009263"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2400)
                     /locus_tag="VD788_11830"
     CDS             complement(<1..2400)
                     /locus_tag="VD788_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HXV76995.1"
                     /translation="MGIPVDPAGRRRRPAAIAALLVASLAAGAPCADDEVTLDAAREL
                     HRAGKLTEALAAYREVAARAAQTDPLSAAIAHNNACVLLLNTGDHTAAVAECRSALEL
                     ARRLDDPRRLGRTLNNLALALQYVGEFDEATERFRDALEINEEQGDVEGQVINWANLG
                     VVATEAGRYADALRCHEHAQQLAERHPEQPWAAEQRQVACINRAVVLERIGAYDEALE
                     LYAEQLAAGSRLDTGRRANLVVNTGVIYRNLGDPVRALAAFESAAASFRELNDRAALS
                     NAILNQALVLHLNLERPADAEVAYRRALELTRQTGDRPEEIRVLCSLGRLLLEHERLV
                     EAGEAFDACRAVAAATDSSEGRWRAAEGAARVAAASGEIDRALDAVERAIAEIEAVRV
                     SLARSELRQHYFGEKRSIYAFAVELLATRALGGAGTAPAERALELAQRAKARSLLDAI
                     GPAGPHSEPLSAGGIAERIGDDLLLEYFFGERRLYGWAVRAGRVRIFDAGPAELRRRE
                     IDAVHAALERGDRAPPGEIGALSAALLAEAEILDRGAERTWIAPDGRLHFLPFELLPL
                     PGGDEPLIRRTEVGYLPSASILALSPRTEPGPATGFAGIGAPSLPAGDERFPSPAGVV
                     VRRYGLTALPDAAREIEAIRDSIPGPGEVRVGDDATEENLARLVRQGTRVLHVATHSV
                     VGESVAGGAAIVLTPTETSDGLLQPAEIARLGRAAELTVLASCRSALGPAAEGDALAV
                     LTGAFLAAGSPAVVATLWDVDDAASRVFMEQFYHHLGRGRTPSAALRAAKLAMIDDER
                     WV"
     gene            2458..>2835
                     /locus_tag="VD788_11835"
     CDS             2458..>2835
                     /locus_tag="VD788_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76996.1"
                     /translation="MEIGEAIARASVWFALCCYPAGPFGPVVSTARGKRVARFVWTAG
                     CVAFAAHVVASFGAFYEWSHLVALDETRRRSAAVTGIDSGLGLYLNYLFGLLWMLDAA
                     WWWSDDEAYGRRSRRGLLLFHAFF"
BASE COUNT          372 a         1035 c         1020 g          408 t
ORIGIN      
        1 gacccagcgc tcgtcgtcga tcatcgccaa cttcgccgcg cgcagcgccg ccgacggcgt
       61 gcgaccgcgt ccgaggtggt gatagaactg ctccatgaag acgcggctcg ccgcgtcgtc
      121 gacgtcccac agcgtggcga cgacggccgg tgagccggcg gcgaggaacg cgccggtcag
      181 gacggccagc gcgtcgccct ccgcggccgg tccgagcgcc gaccgacagc tggcgaggac
      241 ggtcagctcg gcggcccgcc ccaggcgcgc gatctcggcc ggttggagca atccgtcgct
      301 cgtctcggtc ggcgtcagca cgatcgcggc gccgcccgcg accgattcgc cgacgacgct
      361 gtgagtcgcg acgtgcagca cccgcgtgcc ttggcgcacc agccgcgcca gattctcctc
      421 ggtcgcatcg tcgccgacac gcacctcgcc gggccccggg atcgagtcgc ggatcgcctc
      481 gatctcacgg gccgcgtcgg gcagcgcggt gagcccgtac cggcgaacga cgacgccggc
      541 gggggacgga aaacgctcgt cgcccgcagg caagctcggt gcaccgatgc cggcgaaccc
      601 cgtcgccggt cccggttcgg tccgcggtga cagcgcgagg atcgaggcgc tcggcaggta
      661 gccgacttcc gtgcggcgga tcaacggctc gtcccctccc ggcagcggca gcaactcgaa
      721 cggcaggaaa tgcagccggc cgtcgggcgc gatccacgtg cgctcggccc cccggtctaa
      781 gatctcggcc tctgcgagca gcgcggccga cagcgccccg atctcgcccg gtggcgcgcg
      841 gtcgccgcgc tcgagcgcgg cgtgtaccgc gtcgatctca cggcgccgca gctcggccgg
      901 gccggcatcg aagatccgca cgcggccggc ccgcacggcc caaccgtaca accgtcgttc
      961 accgaagaag tactcgagca gcaggtcgtc gccgatccgc tcggcgatcc cgccggcgct
     1021 caacggctcg ctgtgtggac cggccggacc gatcgcgtcg agcagcgagc gggccttcgc
     1081 ccgctgcgcg agctcgagcg cgcgctcggc cggagccgtt ccggccccgc ccaaggcacg
     1141 cgtcgccaac agctcgacgg cgaaggcgta gatcgaccgt ttctcgccga agtagtgctg
     1201 tctcagctcg ctccgagcga gactgacgcg aacggcctcg atctccgcga tcgcccgttc
     1261 gacggcgtcg agcgcgcggt cgatctcacc gctcgccgcc gcgacgcgcg ccgctccctc
     1321 cgccgcgcgc cagcgaccct cgctcgagtc ggtggccgcg gcgacggccc ggcaggcatc
     1381 aaacgcctcg ccggcttcga ccagacgctc gtgctcgagc agcagccggc cgagcgagca
     1441 gagcacgcgg atctcctccg gtcggtcgcc ggtctgacgc gtcagctcga gggctcggcg
     1501 gtacgcgacc tccgcgtccg ccggtcgctc gaggttcaga tggagcacca gcgcttgatt
     1561 gagaatcgcg ttcgacagtg ccgcgcgatc gttcaactcg cgaaagctcg cggcggccga
     1621 ctcgaaggcg gcgagggccc gtaccggatc gcccaggttg cggtagatca cgcccgtgtt
     1681 gaccaccaga ttggcgcggc gaccggtgtc gagtcgtgaa ccggcggcga gttgctcggc
     1741 gtacagctcg agtgcctcgt cgtaggcgcc gatccgttcg aggacgaccg cgcggttgat
     1801 gcaggcgacc tgccgttgct ccgcggccca cggctgctcg gggtgccgct ccgccagctg
     1861 ttgggcgtgc tcgtggcaac gcagcgcgtc ggcgtagcga ccggcctcgg tcgcgacgac
     1921 cccgaggttg gcccagttga tcacctggcc ctcgacgtcg ccctgctcct cgttgatctc
     1981 gagcgcgtcg cggaagcgct cggtcgcttc gtcgaactcg ccgacgtact gcagcgccag
     2041 cgcgaggttg ttgagcgtgc ggccgagccg gcggggatcg tcgagccggc gggccagctc
     2101 gagcgccgac cggcactcgg ccaccgcggc ggtgtgatcc ccggtgttga gcagcagcac
     2161 gcacgcgttg ttgtgcgcga tcgcggccga cagcggatcg gtctgggcgg cgcgcgcggc
     2221 cacctcgcga taggccgcga gcgcttcggt cagcttgccg gcccgatgca gctcgcgcgc
     2281 cgcgtcgagc gtcacctcgt cgtccgcaca cggcgcgccg gccgcgagcg acgcgacgag
     2341 cagcgcggcg atcgccgccg gacgccgccg ccggccggcc ggatcgacgg ggattcccac
     2401 ggcgcgattg tcgcacgatt cgtcgtcggc acggacaagg gctagactcg tcggatcgtg
     2461 gagatcggcg aggcgatcgc gcgggccagc gtctggttcg cgctgtgctg ctaccccgcc
     2521 gggccgttcg gtccggtcgt ctcgaccgcc cgcgggaagc gggtcgcgcg attcgtctgg
     2581 accgccggct gcgtggcgtt cgcggcccac gtcgtcgcat cgttcggagc gttctacgag
     2641 tggagccacc tcgtcgcgct cgacgagacc cggcgccggt ccgccgcggt gaccgggatc
     2701 gattccggtc tgggcctgta cttgaactat ctgttcggac tgctgtggat gctcgacgcg
     2761 gcgtggtggt ggagcgacga cgaggcctac gggcggaggt cgcggcgggg gctgttgctg
     2821 ttccacgcgt tcttc
//
LOCUS       DASPGN010000408         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1009291, whole genome shotgun sequence.
ACCESSION   DASPGN010000408 DASPGN010000000
VERSION     DASPGN010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009291"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..421)
                     /gene="rplS"
                     /locus_tag="VD788_11840"
     CDS             complement(74..421)
                     /gene="rplS"
                     /locus_tag="VD788_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXV76997.1"
                     /translation="MSTIIESIERRQLREVPAFKAGDTVRVHFKVIEGNRQRLQVFEG
                     ICIKRQGSGVRETFTVRKNSFGVGVERTFPLHSPKIEKLEVAMVGDVSRAKLYYLRGR
                     VGKKARIRELRQS"
     gene            complement(715..1578)
                     /gene="lepB"
                     /locus_tag="VD788_11845"
     CDS             complement(715..1578)
                     /gene="lepB"
                     /locus_tag="VD788_11845"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXV76998.1"
                     /translation="MSGIDERDPPRRTDDGHDGPAGGTFAPAERPLEPWGADGEPPED
                     GRAGDWPTTPAEIPLVDDTGLEPGGPAAEGRTAAGARRHSRNPVDRLTRGLPDPIRVV
                     VDWLVTIVGAVAIVLLVKAYVVNPYRIPSSSMEPTLHCAQPASGCEARFSDRVLANRF
                     IFHIRDPRRGEIVVFETPDAARIKCGAGGTFVKRIVGLPGETVEVRLERGEGYVFVNG
                     RKLDEPYLDAGRRSGEEFGPVKVPESGYFMMGDNRSQSCDSREWGSVPRDNLIGKVFA
                     TYWPPNRISLR"
     gene            complement(1575..2231)
                     /locus_tag="VD788_11850"
     CDS             complement(1575..2231)
                     /locus_tag="VD788_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV76999.1"
                     /translation="MRIDVFTQVPHAFGWLTEQRPLATVLGTELELRLLNYRDTTPLR
                     AGQVDDDPYGGGAGMVLRVDVVDTALEAVYGDDRPGRIVALTPQGRQLDQALVEELAI
                     EPRIALLSSRFEGFDERIVSHLCTDALSIGPYVLSGGELPAMVLLDAIARRLPGALAD
                     GSGDVESFSRALEGGIEYPHYTRPAQYRGWDVPDVLLSGDHGRIESWRREQSRVRSAG
                     "
     gene            complement(2415..>2805)
                     /locus_tag="VD788_11855"
     CDS             complement(2415..>2805)
                     /locus_tag="VD788_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77000.1"
                     /translation="SDDEGRWQVGASVVVDGEVATVTLTRRVGGGRRAIRLDRAVPRG
                     AVLAVRLGDLPPAAPDSYYAFQLVGLAVEDDDGRAVGVVTEVHTGVANDNLELDDGRL
                     VPLIEDAVREIDVDGGRIVVARGFVLD"
BASE COUNT          426 a         1021 c          962 g          396 t
ORIGIN      
        1 gaggaccaca cagacgccga acgtcagcac ccagcgcacg aacggaatct agtcctgatc
       61 tcgctccgcg agatcaggac tggcggagct cgcggatccg cgccttcttg ccgacgcgcc
      121 cgcggaggta gtagagcttc gcccggctga cgtcgcccac catggcgacc tcgagcttct
      181 cgatcttcgg cgagtgcagc gggaacgtcc gctcgacgcc gacgccgaac gagttcttgc
      241 gcacggtgaa cgtctcccgc acgcccgagc cctggcgctt gatgcagatc ccctcgaaga
      301 cctggaggcg ctggcggttc ccctcgatca ccttgaagtg cacgcggacg gtgtcaccgg
      361 ccttgaacgc ggggacctcg cgcagctggc gccgctcgat gctctcgatg atcgtgctca
      421 tgagaaaagg gtcggccgtc ggccggcgac ggatgatagc aacggtgcca cgcgtcgccg
      481 acgcgacgcg cggcacctgg acaacagcca tgctcgatgg ggagccgaga gtgcgtgctt
      541 cttctggccg cagccgcgga gcggatgcgg ctcgcgagca agcgaaacga gatcctgccc
      601 ggcggccgcc aagaggcagt tgcggccgcg aagcggacgc cttcagggat gtgccccgcc
      661 gcaggcgggg atccagcggc aggattccgc tcccgcggaa tcctgcttcg cgggctaacg
      721 aagactgatt cgattcggtg gccagtaggt cgcgaagacc ttgccgatca ggttgtcgcg
      781 cggcacggac ccccactcgc gcgagtcgca cgactgcgag cggttgtccc ccatcatgaa
      841 gtacccgctc tccggcacct tgaccgggcc gaactcctcg cccgagcggc ggcctgcgtc
      901 gagatacggc tcgtcgagct tccggccgtt gacgaagacg tagccctcac cgcgctcgag
      961 ccgcacctcc acggtctcgc ccggcagccc cacgatccgc ttcacgaacg tcccccctgc
     1021 gccgcacttg atgcgcgccg cgtcgggggt ctcgaacacg acgatctcgc cgcgccgcgg
     1081 atcgcggatg tggaagatga agcggttcgc gagcacgcga tcggagaagc gcgcctcgca
     1141 gccggatgcg ggctgtgcgc agtggagagt cggctccatc gacgacgacg gaatgcggta
     1201 cgggttgacg acgtacgcct tgacgagcag cacgatcgcc accgcgccga cgatcgtcac
     1261 cagccagtcg acgacgacgc ggatcggatc cgggaggcct cgcgtcaacc ggtcgaccgg
     1321 gtttcgcgag tgacggcggg cgcccgcggc cgtacgaccc tcggcggcgg gtccaccggg
     1381 ctcgaggccc gtgtcgtcga cgagcggaat ctccgcgggc gtcgtcggcc agtcaccggc
     1441 gcggccgtcc tcgggtggct ccccgtcggc tccccacggc tcgagagggc gttccgcggg
     1501 agcgaatgtc ccgccggcag ggccgtcgtg accgtcgtcc gtccgccggg gcgggtcgcg
     1561 ctcgtcgatc ccgctcatcc agcgctcctg acccggctct gctcgcggcg ccagctctcg
     1621 atccggccgt ggtcgcccga gagcaacacg tcgggaacgt cccagccgcg gtactgcgcc
     1681 ggccgcgtgt agtgcgggta ctcgatgccg ccctcgagtg cgcgcgagaa gctctcgacg
     1741 tcgccggagc cgtcggcgag cgccccgggc agcctcctcg cgatcgcgtc gagcagcacc
     1801 atcgccggca gctcgccacc cgagaggacg tacgggccga tggagagcgc gtcggtgcag
     1861 aggtgcgaca cgatccgctc gtcgaagccc tcgaaccggg aggagaggag ggcgatgcgc
     1921 ggctcgatcg cgagctcctc gacgagcgcc tggtcgagct gccgcccctg cggggtgagc
     1981 gcgacgatcc ggccgggccg gtcgtcgccg tacaccgcct cgagcgcagt gtcgacgacg
     2041 tcgacacgca gcaccatgcc cgcgccgcca ccgtacgggt cgtcgtcgac ctggcccgcg
     2101 cggagcgggg tcgtgtcgcg gtagttcaag agccgcagct cgagctcggt cccgagcacg
     2161 gtcgcgagcg gccgctgctc cgtgagccag ccgaacgcgt gcgggacctg cgtgaagacg
     2221 tcgatgcgca ccggcgaccc agggtacccc tagacggttg accgggaaat cgaccagggc
     2281 ggcttgcagg cgccacgcac cgcgacgctg cgtcctcggt cgcaccgaga tgcccacata
     2341 tcggcgctcc ctgcgtcctt gcgtcgcggc acgtgccgcg tgccatccac cgtgaccatt
     2401 tcccggtcaa ccatctagtc gagcacgaac ccgcgggcga ccacgatccg gccgccgtcg
     2461 acgtcgatct cgcgcaccgc gtcctcgatc agcggcacga gccgcccgtc gtcgagctcg
     2521 agattgtcgt tcgcgacacc cgtgtgcacc tctgtcacca cgccgaccgc gcggccgtcg
     2581 tcgtcctcga ccgccaggcc cacgagctga aaggcgtagt agctgtccgg cgccgccggt
     2641 gggagatctc ccagccgcac cgcgagcacg gcaccgcgtg gcacggcgcg gtcgagccgg
     2701 atcgcgcggc ggccgccgcc gacgcgacgg gtgagcgtga cggtcgcgac ctctccgtcg
     2761 acgacgaccg aggctccgac ctgccagcgt ccttcgtcgt cgctc
//
LOCUS       DASPGN010000409         2925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_542001, whole genome shotgun sequence.
ACCESSION   DASPGN010000409 DASPGN010000000
VERSION     DASPGN010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542001"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /gene="ccsA"
                     /locus_tag="VD788_11860"
     CDS             complement(<1..1471)
                     /gene="ccsA"
                     /locus_tag="VD788_11860"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXV77001.1"
                     /translation="MNLGDLLIKLSALFAVVAFVEACRWARGRTGSRRRFRWAYHGMT
                     LTLLAASALLVLAILRHDFRFEYVIGYSSRDLPLIYLLSAFWAGQQGTYLLWGLVGAL
                     FGYFLFRERAWEQATVMAFYLPTVAFMHLLMLDPNGNPFRLATSVPPDGRGLNPLLQD
                     PWMASHPPAIFVGYVAATIPAVLALVALVKRREEHWVSPALRWTLLSFVLLGAGIVLG
                     GFWAYKVLGWGGYWGWDPVENASLIPWIVVTALVHGLIVQKATGALPRTNLALALAGY
                     ATVFYATFLTRSGVLADFSVHSFPAGSIYRILLGIQLAIVAVSLVLFFRRWSIGARDV
                     PLALAWPLVLAVAVVLFVLSAGLVLIGTSWPIITTWLGKPSSFGPSWYNTVSLPIYVA
                     LLALLGVAPLLAWARRPIALVRRRLALTVAAAMLATAIALALGSRGLWPLALFFVAAA
                     ALVANALRLVEVGRGRWLNSGAALAHIGFALMLLGIAGSS"
     gene            1646..1906
                     /locus_tag="VD788_11865"
     CDS             1646..1906
                     /locus_tag="VD788_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77002.1"
                     /translation="MTQPAQQPCGRCAEHARDAVGRFVTQPRRGRGESLRRHRVAALA
                     YADASDGSRTLFADGQAVERLRTLIAEGHLAFVRDAYGSART"
     gene            complement(1885..2562)
                     /locus_tag="VD788_11870"
     CDS             complement(1885..2562)
                     /locus_tag="VD788_11870"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXV77003.1"
                     /translation="MQRSWCPACSTNHDGLAGCPGELTPVGVERPGWRITVETPHGLE
                     AYGVLVAQCEGLWRARVVTFPNVLWKAPGLAGSMKFVGGTGRQAMDRAIEFVRRHCTE
                     RGYRIRGDRFLEPERQLAGRSAVVVPAPRKVRFLPLRFGVVRPSEPAGTANLSESGIY
                     IITPAPLDPGRSLTMSVWIPSNLRVGLSGRVVWMTKMPRFGRPPGMGVCLAEPPPSYL
                     GYVRALP"
     gene            complement(2695..>2925)
                     /locus_tag="VD788_11875"
     CDS             complement(2695..>2925)
                     /locus_tag="VD788_11875"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HXV77004.1"
                     /translation="YLHDGRATTLTEAILAHGGEAEGARDGFASLTDSAKADVLIFLR
                     TLRTPDDLEQSESIQNPGGRLARDRGTFVGRN"
BASE COUNT          514 a         1033 c         1023 g          355 t
ORIGIN      
        1 acgagctgcc ggcgatgccg agcaacatca gcgcgaagcc gatgtgggcc aacgctgcgc
       61 cggagttcag ccaacgtcca cgaccgacct cgaccagacg cagggcgttt gcgacgagcg
      121 ccgccgccgc gacgaagaac agcgcgagcg gccacagccc gcgcgagccg agcgcgagcg
      181 cgatggcggt ggccagcatc gccgcagcga ccgtcagcgc cagacgccgg cggacgagcg
      241 cgatcggtcg ccgggcccag gccaacagcg gcgcgacgcc gagcagtgcg agcagcgcga
      301 cgtagatcgg caagctgacg gtgttgtacc aggacggtcc gaacgacgac ggcttgccga
      361 gccaggtggt gatgatcggc caggacgtcc cgatcagcac cagtcccgcc gacaggacga
      421 acagcacgac ggcgacggcc agcaccagcg gccaggccaa cgccagcggg acgtcgcggg
      481 cgccgatcga ccagcggcgg aagaacagga ccagcgagac cgcgacgatc gccaactgga
      541 ttccgagcag gatccggtag atgctgccgg ccgggaacga gtgcaccgag aagtcggcga
      601 gtacgccgct tcgcgtcagg aacgtcgcat agaacacggt cgcgtagccc gccagtgcca
      661 gcgccaggtt ggtgcgcggg agcgcgccgg tcgccttctg cacgatcaac ccgtgaacca
      721 gcgcggtgac gacgatccac gggatcaacg acgcgttctc gaccgggtcc cagccccagt
      781 agccgcccca gccgagcacc ttgtaggccc agaatccacc gagcacgatt ccggcgccga
      841 gcagcacgaa cgacaacagc gtccagcgca gcgccggcga gacccagtgc tcctcgcgtc
      901 gtttgacgag cgccaccaga gccagcacgg ccgggatcgt cgccgcgacg tagccgacga
      961 agatcgcggg tgggtggctc gccatccacg ggtcctgcaa cagcggattg agcccgcggc
     1021 cgtcgggggg cacgctcgtc gcgaggcgga acgggttgcc gttcgggtcg agcatcagca
     1081 ggtgcatgaa cgcgacggtc ggcagataga acgccatcac cgtggcctgc tcccacgccc
     1141 gctcgcggaa caggaagtag ccgaacagcg cgccgacgag accccacagc aggtaggtgc
     1201 cctgctggcc ggcccagaag gccgacagca gatagatcag cggcaggtcg cgcgaggagt
     1261 agccgatgac gtactcgaag cggaaatcgt ggcgcaggat ggcgagcacc agcagcgccg
     1321 acgcggcgag cagcgtcagc gtcatgccgt gataggccca gcggaagcga cgccgggagc
     1381 ccgtccggcc gcgcgcccaa cggcaggctt cgacgaaggc gacgacggcg aacagcgcgg
     1441 atagcttgat cagcaggtcc ccgagattca tggtggccac tcctccgatc gttgtgggcc
     1501 ccggtcggat ctccttgaga gcaaaacgac tgccaagacg cgtgttgccg agaaccgcgg
     1561 cttacgcggc aaaatccgat ccttttagcc ccgtttcgct cgcccggccg ggggagttgc
     1621 ccctgccccg tcgaatctgc gcgttttgac ccaaccggct cagcagccct gcggccggtg
     1681 cgccgagcac gcgcgagatg cagtcggacg gttcgtcacc cagccccgac gcggtcgcgg
     1741 ggagagcctc aggcgccacc gcgttgcggc tctggcctac gccgacgcga gcgacggcag
     1801 ccgcacgctg ttcgccgacg gccaggcggt cgagcggcta cggacgctga tcgccgaggg
     1861 gcacctcgcg ttcgtgcgtg acgcttacgg cagtgcgcgc acgtagccga ggtagctcgg
     1921 cggtggttcg gcgaggcaca cccccatacc gggaggacgc ccgaaccgcg gcatcttggt
     1981 catccagacg actcgcccgc tgaggccgac ccgcaggttc gacgggatcc acaccgacat
     2041 cgtcagcgag cggccggggt cgagcggcgc gggcgtgatg atgtagatcc cgctctcgga
     2101 cagattcgcc gtccccgcgg gctcggacgg tcgtaccacg ccgaagcgca gcggcagaaa
     2161 ccgcaccttg cgcggcgccg ggaccacgac cgcggatcgg ccggcgagct gccgctccgg
     2221 ttcgaggaag cgatcgccgc ggatccggta gccgcgctcc gtgcagtggc gcctgacgaa
     2281 ctcgatcgcg cgatccatcg cctggcggcc ggtgccgccg acgaatttca tcgatccggc
     2341 gaggcccggt gccttccaca gcacgttggg gaaggtgacg acgcgcgcgc gccagagacc
     2401 ctcgcactgc gcgacgagca cgccgtaggc ctcgaggccg tgcggcgttt cgaccgtgat
     2461 ccgccacccc ggtcgctcga cgccgacggg tgtcagctcg ccgggacaac cggccaggcc
     2521 gtcgtgattc gtgctacacg cgggacacca gctccgctgc atgcggcgac ccctgtctcg
     2581 acggaccgcc ggctcgacgg ccccacaggg gaaaaaccta gtcccgcgcg ccggcggatc
     2641 aagcgtcgcc cgtgcccgcc gtgggacggc ggccgagccg ccgcgctcgc gcccctagtt
     2701 tcgcccgacg aaggtgccgc ggtcgcgcgc caggcgtcca cccgggttct ggatcgactc
     2761 gctctgctcc aggtcgtccg gcgtgcggag cgtacgcagg aagatcagca cgtcggcctt
     2821 tgccgaatcg gtgagcgagg cgaagccgtc gcgcgcgccc tcggcctcgc cgccgtgggc
     2881 cagaatcgcc tcggtcagcg tcgtcgcccg gccgtcgtgc aggta
//
LOCUS       DASPGN010000410         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_542207, whole genome shotgun sequence.
ACCESSION   DASPGN010000410 DASPGN010000000
VERSION     DASPGN010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542207"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="VD788_11880"
     CDS             complement(<1..1270)
                     /locus_tag="VD788_11880"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXV77005.1"
                     /translation="MLDREHVLRALFERLSRRVVLERRLPADLGGYAILVSPDASLRF
                     WFRDLAAVDSVLLAAARELVRPGDVVWDVGANVGLFSFAAAGRSRPDGRVLAIEPDPW
                     LAQLVNRSATLNPDLLGRFNVLQAALTERAGDAVLHVARRGRAANYVAGFMPSTQTGG
                     VREALAVRAVTLDALLDDWPRPDVLKIDVEGAEASVLRGARRVLHEVRPRILCEVSQA
                     NRYEVSAAFDRAAYAMYDVAQPAGRRERIECCAWNTIALPGRDGARRERAGVVVTDRC
                     PNPACGTTDVFAVLERSCSVRLGGRIETVDFRLACCRVCGLGYVDPPSAPHVLDAFDP
                     SDYAYWDAPRERLSHATRIKYELARGRHLQQLRRGFSARLGSRIARLTDRDFDRLRLE
                     HVLEHQPAPIETLAALRGKLRPGAHMVLTVP"
     gene            complement(1245..>2204)
                     /locus_tag="VD788_11885"
     CDS             complement(1245..>2204)
                     /locus_tag="VD788_11885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV77006.1"
                     /translation="AVFARLRERDYDLVEFFGGEFGLVTWRLRRVGQRPFVVAHTDGF
                     ELLASEREDAYAPPRTSSDRLRRWFRRHAHDRLSRAAFTHADAFVTGCELDRKCVLEL
                     RMFAPERTAVVSPGVDEEYLGAREPAGRETRIAFTGSWIPRKGIRYVVETMNELRRAS
                     PELRLDLYGTAASAEAVLQDFAPEVRERVTVHGRLSNAELADGLSRAMVFLFPAQYEG
                     FGMALAEAMACGCAPVTTPTGFGAELRDGKEALLCAFDDVGAMKRSVRALLVDSDLRS
                     RVAAAARRRVAALSWPTQIGKLEATYASWLAQRAAHAGSRARA"
BASE COUNT          395 a          776 c          753 g          280 t
ORIGIN      
        1 aagggaccgt gagcaccatg tgcgcgccgg ggcgcagctt gccccgcagc gccgcgagcg
       61 tctcgatcgg tgcgggttga tgctcgagaa catgctcaag gcggaggcgg tcgaagtcgc
      121 gatcagtcag gcgcgcaatg cgcgaaccca gccgagcgga gaatccgcgt cggagctgct
      181 gcaggtgtcg cccgcgcgcg agctcgtact tgatccgggt cgcgtgcgaa aggcgctctc
      241 ttggagcatc ccagtacgcg tagtcggatg gatcgaacgc gtctagcacg tgaggtgcgg
      301 acggcgggtc gacgtacccg agaccacaga cgcgacagca ggccaagcgg aagtcgaccg
      361 tctcgatgcg cccgccgagg cgaacgctgc aactgcgctc aagcacggcg aagacgtcgg
      421 tggtgccgca cgccgggttc gggcagcgat cggtcacgac gacgccagca cgctcgcgtc
      481 gggcaccgtc tcgaccgggg agggcaatcg tgttccaagc gcagcactcg atgcgctcgc
      541 ggcgccccgc gggttgcgca acatcgtaca tggcgtacgc cgcgcgatcg aacgcggcgg
      601 agacctcgta ccgattggcc tgggatacct cgcagagtat ccgcgggcga acttcatgca
      661 gcacgcgccg tgcgccgcgc agcaccgacg cctccgcgcc ctcaacgtcg atcttgagca
      721 cgtcgggcct gggccagtcg tcgagcagcg cgtcgagcgt cacggcgcgc accgcgagcg
      781 cctcgcggac gcctccggtc tgggtcgaag gcatgaagcc cgcgacgtag ttggccgcgc
      841 ggccgcgcct cgcaacgtgc agcaccgcgt caccggcgcg ctcggtcagc gccgcctgca
      901 gcacgttgaa gcgtccaaga aggtcaggat tgagcgtcgc ggaacgattc acgagctgcg
      961 caagccaagg gtccggctcg atcgccagca cccgaccatc gggacgcgac cggcccgcgg
     1021 ccgcgaagct gaacaggccg acgttggcgc ccacgtccca gacgacgtca cccggtcgga
     1081 cgagctcccg tgcggcggcg agcagcacgg aatccacggc ggcgaggtcg cgaaaccaga
     1141 atcgcagtga agcatcgggt gagaccagga tggcgtagcc gccgaggtcg gcggggagcc
     1201 ggcgctcgag caccaccctc cggctcagcc gctcgaacag cgccctaagc acgtgctcgc
     1261 gatccagcat gcgcggctct ctgagccagc cacgacgcgt acgtcgcctc cagcttgccg
     1321 atctgcgtcg gccacgacaa ggcggcgacc cgccgccgtg ccgcggccgc aacgcgcgaa
     1381 cgcaggtccg aatcgacgag cagtgcgcgg accgaccgct tcatggcacc cacgtcgtcg
     1441 aacgcgcaca gcagcgcctc cttgccatcg cgcagctccg cgccgaaccc ggtcggtgtc
     1501 gtcaccgggg cgcagccgca ggccatcgcc tccgccagtg ccattccgaa gccctcgtac
     1561 tgagcgggaa ataggaacac catcgccctc gaaagcccgt ccgcaagttc ggcgttcgac
     1621 aggcggccgt ggaccgtaac ccgttcgcgc acctcgggcg cgaagtcctg gagcaccgcc
     1681 tccgcagacg cggcggtccc gtacagatcg agtcgcagct cagggctcgc ccgccgcagc
     1741 tcgttcatcg tttcaacgac gtagcggatg cccttgcgag gaatccacga acccgtgaac
     1801 gcgatgcgcg tctcgcgccc cgcgggctcg cgcgcgccaa gatactcctc gtccacaccc
     1861 ggcgagacga cggcagtgcg ttcgggcgcg aacatgcgca gctcaagcac gcacttgcga
     1921 tcgagctcgc agcccgtgac gaacgcgtcg gcatgggtga acgccgcgcg cgagaggcga
     1981 tcgtgggcat gacggcgaaa ccagcggcgc agacggtccg acgacgttcg cgggggcgcg
     2041 tacgcgtctt cgcgttcact cgcgaggagc tcaaaaccgt ccgtgtgggc aacgacgaag
     2101 ggtcgctgcc ccacgcggcg aaggcgccag gtgacgagcc cgaactcgcc gccgaagaac
     2161 tcgacaagat cgtagtcgcg ctcgcgcagg cgggcgaaaa cggc
//
LOCUS       DASPGN010000411         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1478387, whole genome shotgun sequence.
ACCESSION   DASPGN010000411 DASPGN010000000
VERSION     DASPGN010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478387"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VD788_11890"
     CDS             complement(<1..349)
                     /locus_tag="VD788_11890"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXV77007.1"
                     /translation="MTDESSTAPRHPIQVASRRTGLSQDLIRAWERRHAAVSPERTST
                     NRRLYSDADIHRLRLLHGAIAGGRRIGDVAGLSLAELERLVSSDAEGSAPRSSPARAA
                     PAPAAARHLARAVH"
     gene            complement(360..2168)
                     /locus_tag="VD788_11895"
     CDS             complement(360..2168)
                     /locus_tag="VD788_11895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXV77008.1"
                     /translation="MIPLEDFFRKPDKIMVRLSPSGRYLAWLEPYERRMNVVVRDSAT
                     GEQRRVTDSTTRDIEGLTWVSDDRLVYARDRGGDENHHLYAVGRDGSNPLDLTPFEGV
                     KCSIVDDLEDQDDQILFQMNKRAREVFDVYRLDTASGEMQRIAENPGNVQSWITDHAG
                     RLRLAVTTDGVNTSILCREREADGWTTVATYDFKEYASPRLFTFDNRSIIVSSNLGRD
                     KRAIAEYDLRAGGESRVIFEHAEVDLDTVLHSRARKRLTGVAFRVDRLGYRFLDDERE
                     RIQRFVDDRLPGAENTVTSSSKDETRFVVHSGSDRDLGGYYLLDARSMQLTKLFDISP
                     WLDADRLAPMEPIQYRSRDGLLIRGYLTQPRERPHRPVPLVVHPHGGPWARDSWGFDP
                     EVQFLADRGFAVLQMNFRGSAGFGRRFLEAGFGQWGLAMQDDITDGVRWAIEQRIADP
                     ERVAIYGGSYGGYAALSGLTRTPELYACGVSYVGVSNLFSWIAAIPPYWKQYLEMLHE
                     MVGHPERDAERLRATSPLYNADKIGVPLLVAQGANDPRVRKPESDQIVEALRRRGVEV
                     DYLVKDDEGHGFQNEENRFEFYRALEAFLGRHLASG"
     gene            complement(2165..3037)
                     /locus_tag="VD788_11900"
     CDS             complement(2165..3037)
                     /locus_tag="VD788_11900"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV77009.1"
                     /translation="MSLTGLRFALFAVLMLATLLFVVWVFQRRLIYLPLGQSVPAVDE
                     LLPGAEPVRYTTADGLALDGWFQPASTSAPRATVLFFPGNAGNRADRAGIALALARRG
                     FSVLLVDYRGYGGNPGRPSERGLALDAAAALAHLRGRADVDPQRIVYFGESLGGAVAL
                     ALALDHPPAMLALRSPFTSLVDVAALHYPYLPVRWLLADRYPSLERIARVGCPLIVFA
                     GGADRIVPARQSRALYETATVAERRWVAIDDAGHNDRDWLDGERMLDELDRFWDDVRS
                     GPPAAGSPGIPEER"
     gene            3093..3311
                     /locus_tag="VD788_11905"
     CDS             3093..3311
                     /locus_tag="VD788_11905"
                     /inference="COORDINATES: protein motif:HMM:NF043052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HXV77010.1"
                     /translation="MSGTYKLVTLVGTSDKSFEEAIRNAIRDAAGSLRNLAWFEVSEH
                     RGRIDRGEIVEYQVKLQASFKVESSVNP"
     gene            complement(3301..>4038)
                     /locus_tag="VD788_11910"
     CDS             complement(3301..>4038)
                     /locus_tag="VD788_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HXV77011.1"
                     /translation="GSRGLGYQVCDDALRELRGVPQKYGIELPDPQLACAPVDSPEGR
                     RYLGAMSAAGNFAWCNRQLLMWQAREVFAEVFARSWEQLGMSLVYDVAHNIAKLERHR
                     VDGGEREVWVHRKGATRAFPPGHPELPAAYREIGQPVLIPGDMGRASWVLVGRQGSME
                     RTFGTTCHGAGRAMSRTAALRSARGRRIDRELERRGVIARARTLRGLAEEQPEAYKDV
                     DVVVDVVDRAGLSSKVARLRPVGVVKG"
BASE COUNT          622 a         1411 c         1397 g          608 t
ORIGIN      
        1 cgtgcaccgc gcgggccagg tgtcgcgcgg ccgccggcgc cggcgccgcg cgggctgggg
       61 acgaacgggg cgccgacccc tcggcgtcgg aggagacgag ccgctcgagc tcggcgaggc
      121 tcaagccggc gacgtcgccg atccgcctcc cgccggcgat cgcgccgtgc agcaaccgca
      181 gccggtggat gtcggcgtcg ctgtacaggc ggcggttggt cgacgtccgc tcgggcgaca
      241 ccgcggcatg gcgccgctcc catgctcgga tcaggtcctg gctcagaccg gtccggcgcg
      301 aggcgacctg gatcgggtgg cgcggtgccg tgctgctttc gtccgtcatc ggcgcagttc
      361 tagccgctcg ccaggtgtct ccccaagaac gcctcgagcg cccggtagaa ttcgaagcga
      421 ttctcctcgt tctggaagcc gtgtccctcg tcgtccttga ccaggtagtc gacctcgacg
      481 ccgcgacgcc tgagcgcctc gacgatctgg tccgactcgg gcttccggac gcgcgggtcg
      541 ttcgctccct gggccacgag cagcgggacg ccgatcttgt ccgcgttgta caacggcgag
      601 gtcgcgcgca ggcgctcggc gtcgcgttcg ggatgcccga ccatctcgtg gagcatctcg
      661 agatactgct tccagtacgg cggaatcgcc gcgatccagc tgaacaggtt ggacacgccg
      721 acatagctca cgccgcaggc atacagctcc ggcgtccgcg tcagccccga caacgcggcg
      781 tatccgccgt agctgccccc gtagatcgcg acgcgttcgg gatcggcgat gcgctgctcg
      841 atggcccagc gcacgccgtc ggtgatgtcg tcctgcatgg cgagccccca ctggccgaag
      901 ccggcctcga gaaaacgccg gccgaagccg gccgatccgc ggaagttcat ctgcagcacc
      961 gcgaagccgc gatcagcgag gaactgcacc tccgggtcga agccccagct gtcgcgggcc
     1021 cacgggccgc cgtgcggatg aaccacgagc ggaaccgggc ggtgcggtcg ctcgcgcggc
     1081 tgcgtcagat agccgcggat cagcaacccg tcgcggctgc ggtattggat cggctccatc
     1141 ggcgccagcc ggtccgcgtc gagccacggc gagatgtcga acagcttggt cagctgcatc
     1201 gatcgggcgt cgagcaggta gtagccgccg aggtcgcgat cgctcccgga atggacgacg
     1261 aagcgcgtct cgtccttgga gctcgacgtg acggtgttct ccgcgccggg caatcgatcg
     1321 tcgacgaacc gctggatgcg ctcgcgctcg tcgtcgagga agcgataacc cagccgatcg
     1381 acgcggaacg cgacgccggt cagccgctta cgcgcacgcg agtggagcac cgtgtcgaga
     1441 tcgacctccg catgctcgaa gatcacgcgg ctctcgcccc ccgcccgcag gtcgtactcg
     1501 gcgatcgccc gcttgtcgcg ccccaggttc gacgagacga tgatcgagcg gttgtcgaag
     1561 gtgaacaggc gcggactggc atactccttg aagtcgtagg tcgcgaccgt cgtccagccg
     1621 tcggcttcgc gctcgcggca caggatgctg gtgttcacgc cgtcggtcgt gaccgccaac
     1681 cgcagccgtc cggcgtggtc ggtgatccag ctctgcacgt tcccgggatt ctcggcgata
     1741 cgctgcatct ctccgctggc ggtgtcgagt cggtagacgt cgaagacctc cctcgcgcgc
     1801 ttgttcatct gaaacaggat ctgatcgtcc tgatcttcga ggtcatcgac gatgctgcac
     1861 ttgacgcctt cgaacggcgt caggtcgagc gggttcgacc cgtcgcgacc gacggcgtag
     1921 agatggtggt tctcgtcccc gccgcgatcg cgcgcgtaga ccaaccggtc gtcgctgacc
     1981 caggtcaagc cctcgatgtc ccgcgtcgtc gagtcggtga cgcggcgctg ctcgccggtc
     2041 gcactgtccc tgacgacgac gttcatccgc cgctcgtacg gctcgagcca ggccaggtag
     2101 cggccgctgg gcgacagccg aaccatgatc ttgtccggct tgcggaagaa atcttcgagc
     2161 gggatcatcg ttcctccggg atgcccggcg accccgcggc cggcgggccg gatcgaacat
     2221 cgtcccagaa gcgatcgagt tcgtccagca tcctctcgcc gtcgagccag tcgcggtcgt
     2281 tgtgcccggc gtcgtcgatc gcgacccacc ggcgctcggc gactgtcgcc gtctcgtaca
     2341 gcgcgcggct ctgtctggcc ggcacgatgc ggtcggctcc cccggcgaag acgatcagcg
     2401 gacagccgac gcgcgcgatc cgttcgagcg acgggtaacg gtccgcgagc agccagcgca
     2461 cgggcaggta cggatagtgc agcgccgcca cgtcgacgag cgaggtgaac ggcgaacgca
     2521 gcgcgagcat cgccggcgga tggtcgagcg cgagcgcgag cgccaccgcc ccgccgagcg
     2581 actcgccgaa gtagacgatc cgctgcgggt cgacgtccgc gcgacctcgc aggtgggcca
     2641 gcgccgcggc cgcgtcgagc gcgagcccgc gctcgctggg gcgtccgggg ttcccgccgt
     2701 agccgcggta atcgacgagc agcaccgaga agccgcgacg cgccagcgcc agggcgatgc
     2761 cggcgcgatc cgctcggttt ccggcgttcc cggggaagaa cagcaccgtc gcccgtggcg
     2821 cggaggtcga cgccggctga aaccagccgt cgagcgcgag cccgtcggcg gtcgtgtagc
     2881 gaaccggctc ggcgcccggc agcagctcgt cgaccgccgg caccgattgt ccgagcggga
     2941 ggtagatcag ccggcgctgg aagacccaga cgacgaacag cagcgtcgcc agcatcagca
     3001 cggcgaacag cgcgaatcgg agcccggtca gggacatgtt gcgagtctaa caggctgctg
     3061 ggttagaatc ggtcgcgatc cagggagcag aaatgagcgg tacctacaag ctggtcacgt
     3121 tggtcggtac gtccgacaag agtttcgaag aagcgatccg caacgcgatc cgcgacgcgg
     3181 cggggtcgct gcgcaacctg gcctggttcg aggtcagcga gcaccgcggt cgtatcgacc
     3241 ggggcgagat cgtcgaatac caggtcaagc tccaggcgag cttcaaggtc gagtcgtcgg
     3301 tcaacccttg accacgccga ccggacgcag ccgggcgacc ttgctcgaca gtccggctcg
     3361 gtcgacgacg tcgaccacga cgtcgacgtc tttgtaggcc tcgggctgtt cttcggcgag
     3421 tccgcgcagc gtgcgggcgc gcgcgatcac gccgcgtcgt tcgagctccc ggtcgatgcg
     3481 ccggccacgc gcgctcctga gcgcggcggt gcggctcatc gcccgaccgg cgccgtggca
     3541 ggtcgtgccg aacgtccgct ccatgctgcc ctgccgcccg acgagcaccc agctggcgcg
     3601 tcccatgtcg cccggaatca acaccggctg gccgatctcg cggtaggcgg ccggcagctc
     3661 ggggtgcccc ggcggaaagg cgcgggtggc gcccttgcgg tggacccaga cctcccgctc
     3721 gccgccgtcc acgcggtgac gctcgagctt ggcgatgttg tgggccacgt cgtagaccag
     3781 cgacatgccg agctgttccc agctgcgcgc gaagacctcg gcgaacacct cacgggcctg
     3841 ccacatcaac agctgccggt tgcaccaggc gaagttcccg gcggcggaca tcgcccccag
     3901 gtagcgccgg ccctcgggac tgtcgaccgg cgcgcaggct agctgggggt cgggtagctc
     3961 gatgccgtac ttctgcggaa cgccgcgaag ctcacgcaac gcgtcgtcgc acacctgata
     4021 gccgagcccg cgcgaccc
//
LOCUS       DASPGN010000412        16848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1596677, whole genome shotgun sequence.
ACCESSION   DASPGN010000412 DASPGN010000000
VERSION     DASPGN010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 16848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16848
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596677"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VD788_11915"
     CDS             <1..949
                     /locus_tag="VD788_11915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77012.1"
                     /translation="AYENSIMKEGALAGLYQVALVRGEFDRAAQYAEQLDDLSGGRPW
                     TRAMPIETLIRAGEQKKADKATAAALESITEPKERRAFLRELAKGQLAAGHFAEAERT
                     ARQARELDEPNEPDEVTARFLAWSLMNQGKTDEAERVIAEAIAEHPYSADLRVYGSFN
                     ALIAGDAETAERRAAEVLARGPAISAAHVALAYALGQQGKFDRAVPHAERALAMGPDR
                     TSRTLLAWVLIAGEIDLERGLELAVKAVDTPDSYFEAANDLSCMALAEHCLGVAYLKQ
                     GRYDEAVAVLTEASRLRPEHAAIREQLEQASQLSVQSGS"
     gene            complement(934..1560)
                     /locus_tag="VD788_11920"
     CDS             complement(934..1560)
                     /locus_tag="VD788_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HXV77013.1"
                     /translation="MGAPPSMIGIAGGSCSGKSTIAARLAERLGSARAPVVALDSYYR
                     DRRELERLTGSEPNWDDPVALDRALMIDQLVALSRGASVDLPVYDFNEHRRTADTLTI
                     VPGTHVIAEGLFTLRWSELRALWRLSVFVEVDDDTGRRRRIARDTRERGRDRASVERQ
                     WRLSVRPMYRAHVAPTRRYADLALDGTRPVDESLRAIVAQLAPDQDPD"
     gene            complement(1568..2182)
                     /locus_tag="VD788_11925"
     CDS             complement(1568..2182)
                     /locus_tag="VD788_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77014.1"
                     /translation="MEPPAEPQLAPYVIEGARSSRSRCKSCRRKIDKGTLRIGVLIEG
                     PYGTGYLWNHLSCLARTRLDQVEEAYAQQAWNEAKELPVKLPSLDELRKLAEEGARRR
                     EQRKTLPYAEIDPSGRAKCKGCGGPLERGAVRFVLGRAVEFGNQVRTAPVLLHPRCVA
                     AELAQDDRGTDADGFHRAVRDHSEDLVGELLDAALAEIDAAGLR"
     gene            complement(2227..3618)
                     /locus_tag="VD788_11930"
     CDS             complement(2227..3618)
                     /locus_tag="VD788_11930"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HXV77015.1"
                     /translation="MSIYHTVRRLFTEETPPLDPRAGGRRTDGRAEHRLRRDAESILR
                     AAIAAVDPEELVVQAMATHARTIPADGRLWVAGFGKAVIPMARGLHRVVGDRVAGAVL
                     IAPVGAQAYVGPQFDVFSAGHPLPDQGGEAGARAVRQLAAEAEAGDLLICLVSGGGSS
                     LLTLPPDDVPLEEVRIVTDLLLRSGAEIDEINCVRKHIDMLKGGRLAREAAPARVLSL
                     VLSDAADNATEIVASGPTVPDPSRFADALAVLKRYDLWKQVPLAVRGWLDRGRCGEID
                     DTPERNDTVFLNTSNVIIGDGQMAAGAARWAAERLGYETQVLTTALGGAAEEAGCFLA
                     ETARIVRAAQSPEPYPTCLITTGETSLRSRDSRYGSRNQQLALAAALRIDRLDGALVA
                     SMGTDGFDGWCDAAGAIATGTTIERARSAGLDCAAALADGRATSIFERLDDQIVSGPT
                     GTDVADIQIVLMA"
     gene            3847..4554
                     /locus_tag="VD788_11935"
     CDS             3847..4554
                     /locus_tag="VD788_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77016.1"
                     /translation="MGRNAQTAAAVAVHLLTASGGAIALFAIEAISGGRIRSALGWMC
                     AAVVVDGVDGTLARRARVRELLPRIDGALLDNVVDYVNYAIVPAWLIHANGLLPERFS
                     PAGTLVIAVAAAFQFSHTSAKSEDHLFRGFPSYWNVTAFYLLVGDLAPGINLAVVCGL
                     ALLSVVPLYWVYPSRTPTLRRTTIALTVLWGAALITMIVRYPRVDPWLARISLLYVAY
                     YTAVSLWLTYRRRRTTV"
     gene            4562..5323
                     /locus_tag="VD788_11940"
     CDS             4562..5323
                     /locus_tag="VD788_11940"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXV77017.1"
                     /translation="MRRIVVIGDVHGEQQRLARVLSWIADEPPALALLVGDLAVDPPW
                     RAPLRLRERGAHDRSVREVLARVRAALGCPVLFVPGNHDLPDPDPAAVGTNCDGSVVD
                     CAGLRVGGLGGAGPARFGFPYEWGERDASAALAALSAAGERIDLLVCHAPPHGSTLDR
                     IHRGEHVGSRAVREWVVAARPRLAVFGHIHEAWGLERLDGVPCLNAGALGEPFGEEII
                     WSVDWDERAGPLVVRSYHPAAGEFETRDWLGPDGR"
     gene            complement(5320..7512)
                     /locus_tag="VD788_11945"
     CDS             complement(5320..7512)
                     /locus_tag="VD788_11945"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77018.1"
                     /translation="MPVTRSAGQWVDRWLLLGTFVCLAVLALHRLFAYDIWWQIAAGR
                     QIAGHGIPEVDPFSFGFADRPWVEPRWLWCLAVWAVYAGGGVNFLILLKLALLAAAGG
                     LLVAAAAGRPRWAVTLGLSLVLATAHERFMVRPELVSFVALAATLLCVQQFKDGGSSR
                     WLWGLPAMQVVWCNTHTLWVLGPVTLWIVVLAEWVEGRAVDRGVLAPRNDRIAGARLG
                     ALASAALATSATTLVNPYFVRGVLFPLDLLHEIRAGHMFSETIAEFRGPFSDYFFSAD
                     FRTTAFVAAIVVSFAALVANRRRVPLYRPALWAAYLFVAVEAQRNVALFGWVAGFTLM
                     LDLAQWHAAAPRRLTPVVVRATVASFLVVLIPAAASDRFYRSQGSLKRFGFGVSDHRF
                     PRKALAFIREHDLPMPAFHALGDGGYVLFEGGPASVYADGRLEVYGSEILERAFRVTW
                     AGEGLDAESDRTGADTIIVRNDIGYRPLLRRLERSGEWFPVYYDPLHLVYLRGRPATR
                     ERFAQLAFDWSSPPRFALTAPRLPADAPWLVDRTDDGYADERLGSLFAGVGNYELARS
                     HFERAHVLNPRDARTRMFVGLFREAEGRQDEAARLLRSVPPGYLAEAEVHTLAGAVGL
                     WAGNRETALAHFERARELGAAEPAASLLVARAALLAGETERASAALIPLIEVHGAEPE
                     LWNLMAVLEIKRGRPAEAAAHFEHSLELDPDQPSVRNQLRALEHQRQD"
     gene            complement(7503..7922)
                     /locus_tag="VD788_11950"
     CDS             complement(7503..7922)
                     /locus_tag="VD788_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77019.1"
                     /translation="MGIGVRSFLVDRDGSVSRIPWCRMSELFQGRACEEFRRRSGQSV
                     RCAIVFIEFERRRPVRILRTEFNVLYLDERGALDSNAQSQQLRLIGQSLDGEYPPGRM
                     RSNLIDMRPRLARKKLRDKFEWTPSEEVLETIFRRCL"
     gene            complement(8013..8198)
                     /locus_tag="VD788_11955"
     CDS             complement(8013..8198)
                     /locus_tag="VD788_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77020.1"
                     /translation="MLTLFEELNLYVYHSLRGLVLDVVIWCLHPLIYDGRQYGLFRIY
                     LAAVTCKACRSWYGIVD"
     gene            complement(8276..9361)
                     /locus_tag="VD788_11960"
     CDS             complement(8276..9361)
                     /locus_tag="VD788_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77021.1"
                     /translation="MILSKGRVLLVCAIACVGNGAGLAQPAKVPLTGILTDSNQRPVA
                     DFRVILRVAGTSRILVSPPTDDEGSYRLDVDTGINCAPIAVVSPLGKRVALAEMTPVP
                     VLPGTRFDIELEHPIRPARVEWAFRGADRLFVSFVEDAAFVGRQRAQAQLTVADFDRS
                     QSYVSEFLAAYNWRAVPTLEVGGRIGYAGTDFDRGGGASGMTDLEFWGKFLAGSSVKT
                     GTRWAPGAVLTLPTGDADTGLSAEASRLKLFATARHELGRLTLSGNLGLRYNEDATID
                     GTELAGEIAGSLGIAALLPVGDQLVGVAELYFEGKRFEQGQDTGLFLVGANWKPLEHG
                     FFRLAAGFGLEDGAPDFQLVAGYVFDF"
     gene            9491..10072
                     /locus_tag="VD788_11965"
     CDS             9491..10072
                     /locus_tag="VD788_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HXV77022.1"
                     /translation="MIRVTDRAKAKFLEIVRGEGRESDGLRVIVEHGGTARAEFALNF
                     VGPGDGNEDDVVEDVGPFKVHIDPTSAKYLEEASVDYVDGLDDSGFKVDAPHAGIAPP
                     KGPVAEAVRKVLEEKVNPAVANHGGFVELVAVEDDTAYLRFGGGCQGCGMINVTLKQG
                     VEKILFAEVAGISKVMDVTDHAAGTNPYYQPAK"
     gene            10032..11468
                     /locus_tag="VD788_11970"
     CDS             10032..11468
                     /locus_tag="VD788_11970"
                     /inference="COORDINATES: protein motif:HMM:NF040546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HXV77023.1"
                     /translation="MRRVPTPTTSRPSRPALRRIEIRLVSCYELGHQPLAVATAQAAL
                     ERCGFRPAAVDLAVEPLAAEAASRPADLIAISVPMHTALHIGVAAARRLRGAHEAAHI
                     CLYGLYASLNADYLLGAGLCDSIVGGEFERPLVDLARALAGGSPVEEVRGLRLAGRPS
                     PPNVERTPFDLPARGGLPPLERYAKLRIDGGERTAAAIEASRGCLHHCRHCPIPPVYD
                     GRFFVVPRELVLGDVRNLVAAGVRHLTFADPDFLNGPRHTMTLAREIHEEFPHLTFDV
                     TTKIENLLRHRERLPQLARFGCVFVVSAVESLNDTVLEHLAKGHTRRDVFEAQRLVAR
                     AGIALRPSLVPFTPWETLDSYRELLDWIECDALADGIDPVQLSIRLLVPPGSLLERSP
                     AMRPYLRAFEPQRFGHRWDHPDPRMDALQREVARIVARAARHAEPGRATHARIRRAAG
                     VPASPVPTRVPPALAAADPPRLTEPWFC"
     gene            complement(11537..12772)
                     /locus_tag="VD788_11975"
     CDS             complement(11537..12772)
                     /locus_tag="VD788_11975"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXV77024.1"
                     /translation="MPASVLLLIPTTSYKTDDFMRAAARLDVELIVGTDRRQALEAVA
                     AGHTLAFDLRDTARALDDIERLAARRGLHAVLGVDDETTLLATLAAARLGLPHNPIEA
                     VRATRDKYEMRRLFSRAGLFGPAFERLPLDVDPVELASGLSFPGVLKPVGLAGSRGVI
                     RVDDRSGFVRAFRRIAAILDDAEVRRLKGVQRHLLFERYLPGAEYAVEGLLDRGRLHV
                     LALFDKPDPLVGPYFEETLLVTPSRASEGVRRALADETHAGCRALGLVHGPLHAELRV
                     SEGRPWLIEIAARTIGGICSRTLRFGHGRSLEQLVLGQALGDGAILDPVPGAAGVMML
                     PIPARGILREVRGIDAARRVDLVEEVTMTMHRGDEVIPLPEGHRYLGFVFARGSTPEA
                     VEHALRAAGALLKFEIEPV"
     gene            complement(12803..13372)
                     /locus_tag="VD788_11980"
     CDS             complement(12803..13372)
                     /locus_tag="VD788_11980"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HXV77025.1"
                     /translation="MPEVEGSRRRSPIVLLAVVLFVAACGDPRPRAGSAETAPPAAPA
                     PADPQPELPVEPPPSPGLRSAEAAPAGSEDDPARLAGLVWSLRIEPGISTRPIPDGFH
                     ITIAFDLDGAVRGHSGCNDYSGRVRIDRGKLGFGPLASTMKACSAPAMELERSYMAHL
                     EQVNGFAIREERLQLLRGDRPILVFVPTE"
     gene            13570..15552
                     /locus_tag="VD788_11985"
     CDS             13570..15552
                     /locus_tag="VD788_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXV77026.1"
                     /translation="MELLRIVQLLTYSFGAYAFGSALVMTLRPASPRPDDCLARGPSR
                     FARTADLLMLVVCLVWFCTLLAHTLDELRPGRWLSIDLTRLVLMYQFPPLIALVNYAD
                     ARKQTEALAHAAWRTTLVGLWVLAEATLAYALLAVYDVLPAVRPLDVYLGLSISALFV
                     LASVFSSFVVVRGRPHRETQTERSGRRWMLGLYLLMVALVGLIVTAYSLGWPIAEVFA
                     FVGTSLPLIFMFTATYHADRFEFFDLFVKRGLLVLATIVLLTAFFGGVLPRLEAIELG
                     WARPWVYAVALSPAVLALPWVYRKMGAWLDNVWLGRRLTTLEAMKRFLSDLGQGADRR
                     DLVLRAERGLSEIFNAPAQVRLSPERAQPDDAEWLQQAPVVSPDGTVGVIRLGRRANH
                     VPYFSEDRALLGSLADVFSHMLRAVDLHRKKREQEQLARELSLRASRAELRALRAQIN
                     PHFLFNALNTIAALIHQDPARADQTVEQLAEIFRYTLRGSDSEWTPLDDEIDFVRSYL
                     EVERARFGERLSVEITADAEARAHFVPTMMIQTLVENAVKHGVARVRGVARIEVDATV
                     AGDRLVVAVRDSGPGFDRATPASVKRGAGGGGYGLHNVRRRLLGHFGAQAALEVRRDE
                     TRGRTEVRLVFPSAMLERVPRPDAGRSGPETQGAAR"
     gene            15549..16661
                     /locus_tag="VD788_11990"
     CDS             15549..16661
                     /locus_tag="VD788_11990"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXV77027.1"
                     /translation="MTIRALLVDDEPPARARLRAMLDRIDGLQIVGEASNGVEAVEQI
                     GATRPDLVFLDVEMPGLSGIQVAASLAPPRPAIVFCTAYDRYAIPAFDHHATDYVLKP
                     VQAARLERTIARVRETLAHEATLRGELEAATDTQRRLLPQRLPEMRTLDYAGSCRPAK
                     EVGGDYYDFLALDDGGLGLALADVSGKGIFAALLSASLQAKVQALAPHYERVDQLVGQ
                     INRMTHASIAANRYATMFYGRYDDETRALDYVNAGHLPPIVVRRGARPGETRIERLAP
                     SGTVVGLLAEARYRRRRIALRPGDLFCIFTDGLSETRDAAGEEFGCTRLERLLTAHSA
                     ESDAERVTSAIRDELDAFAGARTREDDLTWIVGCVR"
     gene            16658..>16848
                     /locus_tag="VD788_11995"
     CDS             16658..>16848
                     /locus_tag="VD788_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV77028.1"
                     /translation="MSDRPLSVLIVDDERPARERLERLLAGDRRLAVTGHAEDGEQAM
                     DQIVGLRPDIVFLDIQMPG"
BASE COUNT         2556 a         5908 c         5948 g         2436 t
ORIGIN      
        1 cgcctacgag aacagcatca tgaaggaagg cgcgctggcc ggcttgtacc aagtcgcgct
       61 cgttcgtggc gagttcgacc gcgcggcgca atacgccgag caactcgacg acctgagcgg
      121 cggccgcccc tggacgcgcg cgatgccgat cgagacgctg atccgcgccg gcgaacagaa
      181 gaaagcggac aaagcgaccg ccgcggcgct cgagtcgatc accgagccga aggaacgccg
      241 tgccttcctc cgcgagctgg ccaagggaca actcgcggcc ggccatttcg cggaggccga
      301 gcgaacggcg cggcaggcgc gcgagctgga cgaaccgaac gagccggatg aggtgaccgc
      361 acggtttctg gcctggagtt tgatgaacca gggcaagacc gacgaggccg agcgggtgat
      421 cgccgaggcg atcgccgagc atccctactc ggccgacctg cgcgtgtacg ggtcgttcaa
      481 cgcgctgatc gccggcgacg cggagacggc cgagcgtcgg gcggccgagg tgctggcgcg
      541 gggacccgcg atctcggcgg cgcacgttgc gctggcctac gcgctcggcc agcagggcaa
      601 gttcgaccgc gcggtgccgc acgccgagcg cgcgctcgcg atgggacccg accgcaccag
      661 ccgcacgctg ctggcctggg tgttgatcgc cggggagatc gatctcgaac gcgggctcga
      721 gctcgcggtc aaggccgtcg acacccccga ctcgtacttc gaggcggcga atgatctgtc
      781 gtgcatggcg ctcgccgagc actgcctggg cgtggcctac ctcaagcagg ggcgttacga
      841 cgaggcggtc gcggtgttga ccgaggcctc ccgccttcgc ccggagcacg cggcgatccg
      901 cgagcagctc gaacaggcgt cgcagctgtc ggttcagtcg ggatcctgat ccggcgcgag
      961 ctgcgcgacg atcgcgcgca gcgactcgtc gaccggtcgg gtcccgtcca gtgcgagatc
     1021 cgcgtagcgg cgggtcggcg cgacgtgggc ccggtacatc ggtcgaaccg agagccgcca
     1081 ctggcgctcg accgacgccc ggtcgcgccc gcgctcgcgc gtatcccgcg cgatgcggcg
     1141 tcgccggccg gtgtcgtcgt cgacctcgac gaagaccgag agacgccaca gcgcccgcag
     1201 ctccgaccag cgcagcgtga acagcccctc ggcgatcacg tgggtaccgg ggacgatcgt
     1261 cagcgtgtcg gccgtgcgac gatgctcgtt gaagtcgtag accggtaggt cgacgctggc
     1321 accgcgggac aacgcgacga gttggtcgat catcagcgcc cggtcgagcg cgacgggatc
     1381 gtcccagttc ggttcactgc ccgtcagccg ctcgagctcc cgccggtcgc ggtagtagga
     1441 gtcgagcgcg accaccggtg cccgggccga tcccagtcgc tcggcaagcc gtgcggcgat
     1501 cgtgctcttg ccggaacacg agccgcccgc gataccgatc atcgacggcg gcgcgcccat
     1561 cgcggcctca gcgaagaccc gccgcgtcga tctccgccag cgccgcatcg agcagctcgc
     1621 cgacgaggtc ctcgctgtgg tcccgcaccg cccggtggaa cccgtcggcg tcggtgccgc
     1681 ggtcgtcctg cgccagctcg gccgcgacac atcgcggatg caacaggacg ggagcggtgc
     1741 ggacctggtt gccgaactcc accgctcgac cgagcacgaa tcggaccgcg ccgcgctcga
     1801 gcggaccgcc gcaacccttg cacttggccc ggcccgacgg gtcgatctcg gcgtacggca
     1861 gtgttttgcg ttgctcccga cgccgcgcgc cctcttcggc cagcttccgc agctcgtcga
     1921 gcgacggcag cttgaccggc agctccttgg cctcgttcca cgcctgctga gcgtaggcct
     1981 cttccacctg gtcgagccgc gtgcgggcca ggcagctcag gtgattccac aagtagccgg
     2041 tgccgtacgg cccctcgatc aacacgccga tccgcagcgt gcccttgtcg atcttgcggc
     2101 gacagctctt gcagcgcgag cggctcgatc gtgcgccctc gatgacgtac ggggcgagct
     2161 ggggttcggc gggtggctcc atctccacca cagatgataa caactgcccg ggtcggagtc
     2221 tcccggtcag gccatcagca cgatctggat gtcggcgacg tcggtaccgg tcggcccgct
     2281 gacgatctgg tcgtccagcc gctcgaagat cgacgtcgcc cgaccgtcgg cgagtgccgc
     2341 ggcgcagtcg agaccggccg agcgcgcgcg ctcgatcgtc gtcccggtgg cgatcgcacc
     2401 ggcggcgtca caccagccgt cgaagccgtc ggtgcccatc gacgcgacga gcgctccatc
     2461 caaccggtcg atcctcaacg cggcggcgag cgccagctgc tggttgcggg acccgtagcg
     2521 cgagtcgcgc gagcgtaacg aggtctcgcc ggtcgtgatc agacaggtcg gatacggctc
     2581 gggcgactgg gccgcgcgca cgattcgcgc ggtttccgcc aggaagcagc cggcctcctc
     2641 ggccgcaccg ccgagtgcgg tcgtcagcac ctgcgtctcg tagcccagcc tctcggccgc
     2701 ccagcgggcg gcgccggccg ccatctggcc gtcgccgatg atgacgttcg aggtgttgag
     2761 gaagacggtg tcgttccgct cgggcgtgtc gtcgatctca ccgcagcgac cgcggtcgag
     2821 ccagccgcgg acggccagcg gtacctgctt ccacagatcg tagcgcttca acacggccag
     2881 cgcgtcggcg aagcgcgagg gatcgggcac cgtcgggccg gacgcgacga tctcggtcgc
     2941 gttgtccgcc gcatcggaca ggacgagcga caggacgcgg gccggtgccg cctcgcgcgc
     3001 cagacgcccg cccttcagca tgtcgatgtg cttgcgcacg cagttgatct cgtcgatctc
     3061 ggcgcccgac cgcagcaaca ggtcggtgac gatgcgcacc tcctcgagcg gcacgtcgtc
     3121 gggtgggagc gtgagcagcg acgagccgcc acccgagacg agacagatca acagatcgcc
     3181 ggcttcggcc tcggccgcga gctggcggac cgcgcgcgca ccggcctcgc caccttgatc
     3241 cggcagcggg tggccggcgc tgaagacgtc gaactgcggg ccgacgtagg cctgggcgcc
     3301 gaccggggcg atcagcaccg cgcccgccac gcggtcgccg acgacgcggt gtagcccgcg
     3361 cgccatcggg atgacggcct tgccgaaccc ggcgacccac aaccggccgt ctgcgggaat
     3421 cgtgcgggcg tgcgtcgcca tcgcctggac cacgagttcc tcggggtcga cggccgcgat
     3481 cgcggcccgt aggatcgact ccgcgtcgcg ccgcagccgg tgctccgcgc gaccgtccgt
     3541 cctccggccg ccggcccggg gatcgagcgg cggcgtttcc tcggtgaaca ggcgacggac
     3601 ggtgtggtag atcgacatcg gctcccccgg cgggtcgggc tgaacgtagt gtcgccgggc
     3661 ggccgtcgca agaatcacgc ccccctgagc gccgagtgaa accgacggcg gtaccggcgg
     3721 gtcgactacg aaagtgaggt cgagattacg gaatcgaaca cgaatcgacc gctgcggggc
     3781 taggatttcc gcacgccgcg cgcacgggat cgcgcggcgc ggacgagagg tgaggagcgc
     3841 gcgtcgatgg gacgaaacgc gcagacggcg gcggccgtcg cggtccacct gttgacggcg
     3901 agcggtggcg ccatcgcgct gttcgcgatc gaggcgatct cgggcggcag gatccgctcg
     3961 gcgctgggct ggatgtgcgc ggcggtcgtc gtcgacggcg tggacggaac actcgcccgc
     4021 cgtgcgcggg tacgcgagct cctgccgcgc atcgacggcg cgttgctcga caacgtcgtc
     4081 gactacgtga actacgcgat cgtgccggct tggctgatcc acgccaatgg tctgttgccg
     4141 gaacggttct cgccggcggg cacgctcgtg atcgcggttg ccgcggcatt ccagttctcc
     4201 cacacctcgg ccaagagcga ggatcatctc ttccgcggct tcccgtcgta ctggaacgtc
     4261 accgcgttct acctgctggt gggcgatttg gcgccgggga tcaacctggc cgtcgtctgc
     4321 ggcctggctc tgttgagcgt cgttccgctg tactgggtct acccctcgcg cacgccgacg
     4381 ctgcggcgca cgacgatcgc gctgacggtg ctgtggggtg cggcgctgat cacgatgatc
     4441 gtgcggtatc cgcgcgtcga tccgtggctg gcccgcatct cgctgctcta cgtcgcctac
     4501 tacacggcgg tcagcctgtg gctgacctac cgcaggcggc gcacgacggt atagtcggtc
     4561 gatgcggcgg atcgtggtca tcggcgacgt ccacggcgag cagcagcgct tggcccgcgt
     4621 gctgagctgg atcgccgacg aaccgccggc gttggcgctg ctcgtcggcg acctggccgt
     4681 cgatccaccg tggcgggcgc cgctgcgctt gcgcgaacgc ggggcccacg accggtccgt
     4741 ccgcgaagtg ctggcgcgcg tacgggccgc cctcggttgc ccggtgctgt tcgtgcccgg
     4801 caatcatgac ctgccggatc ccgacccggc cgccgtcggt accaactgcg atgggtcggt
     4861 cgtcgattgc gccggccttc gggtcggcgg tctgggcggc gccggaccgg cgcgtttcgg
     4921 cttcccgtac gagtggggcg aacgcgacgc gagcgcggcc ctcgccgcct tgtccgcggc
     4981 gggcgagcgg atcgatctgc tcgtctgcca cgcgccaccc cacgggtcga cgctcgatcg
     5041 gatccatcgc ggcgagcacg tcggcagccg ggccgtgcgg gagtgggtcg tcgcggcccg
     5101 cccgcggctc gcggttttcg gtcacatcca cgaggcgtgg gggctcgagc gactcgacgg
     5161 ggtaccgtgt ctgaacgccg gcgcgctggg cgagccgttc ggcgaggaga tcatctggtc
     5221 ggtcgactgg gacgaacggg ccggaccgct ggtcgtccgg tcgtatcacc ccgcggcggg
     5281 cgagttcgaa acacgcgact ggctcgggcc ggacggccgc tagtcctgcc tctggtgctc
     5341 gagcgctcgc agctggttcc ggacgctcgg ctgatccggg tcgagttcca gggaatgctc
     5401 gaagtgcgcg gcggcctcgg ccggtcgacc gcgcttgatc tcgagcaccg ccatcaggtt
     5461 ccacagctcc ggctcggccc cgtgcacctc gatcaacggg atcagcgcgg ccgacgcccg
     5521 ctcggtctcg cccgcgagca gcgcggcgcg cgcgacgagc agcgacgccg ccggctcggc
     5581 cgcgcccagt tcgcgagccc gttcgaagtg cgcgagcgcc gtctcgcggt tgccggccca
     5641 tagaccgacc gccccggcca gcgtgtggac ctcggcttcg gcgaggtagc ccggcggcac
     5701 cgagcggagc agccgcgcgg cctcgtcctg ccggccctcg gcctcgcgaa acagaccgac
     5761 gaacatgcgc gtccgcgcat cgcgcggatt gagcacgtgc gcccgctcga agtgcgagcg
     5821 cgccagctcg tagttgccga caccggcgaa caggctgccc agacgctcgt cggcgtagcc
     5881 gtcgtccgtt cgatcgacga gccacggcgc gtcggccggc agccgcggcg cggtcagcgc
     5941 gaaccgcggc ggcgacgacc agtcgaacgc cagctgcgcg aaccgctcgc gggtggccgg
     6001 gcggccgcgc aggtagacca ggtgcagcgg atcgtaatag accgggaacc actccccgct
     6061 ccgctcgagc cgacgaagca acgggcggta accgatgtcg ttgcgcacga tgatcgtgtc
     6121 ggcacccgtg cggtccgact ccgcgtcgag gccctcaccg gcccacgtca cgcggaacgc
     6181 gcgctcgagg atctcactgc cgtagacctc gagccgcccg tcggcgtaga cgctcgccgg
     6241 cccgccctcg aacaggacgt acccgccgtc cccgagggcg tggaaggccg gcatcggcag
     6301 atcgtgctcg cggatgaacg ccagcgcttt gcgcgggaag cgatggtcgc tgacgccgaa
     6361 gccgaaacgc ttgagcgatc cctgtgagcg atagaagcgg tcgcttgcgg ccgccgggat
     6421 cagcacgacg aggaacgacg cgacggtcgc ccgcacgacg accggcgtca gccggcgcgg
     6481 cgccgccgcg tgccattgcg ccagatcgag catcagcgtg aatccggcga cccagccgaa
     6541 cagggcgacg tttcgttggg cctcgaccgc aacgaacagg taggcggccc ataatgcggg
     6601 ccgatacaac ggcacccgac gccggttggc gacgagggcc gcgaacgaga cgacgatcgc
     6661 ggcgacgaag gccgtcgtgc ggaagtccgc cgagaagaag tagtcggaga acgggccgcg
     6721 gaactcggcg atcgtctcgc tgaacatgtg gcccgcgcgg atctcgtgga gcaggtcgag
     6781 cgggaacagc acgccacgga cgaagtacgg gttgaccaag gtcgtcgccg aggtcgcgag
     6841 tgcggccgac gccagtgcgc cgaggcgcgc cccggcgatg cggtcgttgc ggggtgcgag
     6901 cacgccccgg tccaccgccc gtccctcgac ccactcggcc agcacgacga tccacagcgt
     6961 gaccgggccg agcacccaca gcgtatgcgt gttgcaccag acgacctgca tcgccggcag
     7021 cccccacagc cagcgcgacg atcctccgtc cttgaattgc tggacgcata gcagggtcgc
     7081 cgccagcgcg acgaacgaca cgagctcggg tcgcaccatg aaccgctcgt gcgccgtcgc
     7141 cagcacgagc gacagaccga gcgtcacggc ccagcgcggc cgacccgcgg cggcggcgac
     7201 gagcagccca ccggccgccg ccaacagagc cagcttgagc aggatcagga agttcacccc
     7261 gccgccggcg tagacggccc agacggcgag gcaccacagc cagcgcggct cgacccacgg
     7321 ccggtcggcg aagccgaagc tgaacgggtc gacctcggga atgccgtgac cggcaatctg
     7381 gcggcccgcg gcgatctgcc accagatgtc gtaggcgaac aggcggtgca gcgcgagaac
     7441 ggccaggcag acgaacgtgc cgagcagcag ccaccggtcg acccactggc cggccgatcg
     7501 cgttacaggc atctgcggaa gatcgtctcc agcacttcct cgctcggtgt ccactcgaac
     7561 ttgtcgcgca gcttcttgcg cgcgaggcgc ggacgcatgt cgatcaggtt ggagcgcatg
     7621 cgccccggcg ggtactcccc atcgaggctc tggccgatca gacggagctg ctggctctgg
     7681 gcgttggagt cgagcgcgcc gcgctcgtcg aggtagagca cgttgaactc cgtgcgcagg
     7741 atgcggaccg gccgacgcct ctcgaactcg atgaagacga tcgcgcagcg caccgactgg
     7801 cccgagcgcc ggcggaactc ctcgcaggcc ctgccctgga acagctccga catgcggcac
     7861 cacgggatgc ggctcaccga cccatcccga tcgaccaaga agctgcgaac gccgatgccc
     7921 atcgtgcgga aggtaccctc gagatccggg aaaagcatag tccaacccgg tcccgggttc
     7981 ctaccccccg tcgggtcggc ccgcggcccc gatcaatcca cgatgccgta ccaactgcgg
     8041 caggccttgc aagtgaccgc ggcgaggtag atgcggaaca ggccgtactg ccggccgtcg
     8101 tagatcagcg gatggagaca ccagatgacg acgtcgagca ccagcccgcg gagcgagtgg
     8161 tacacgtaga ggttcagttc ctcgaacagc gtcagcatgg tactcgattc ccatataccg
     8221 acggccggag ggattgccac tcgactgtaa ctatttcaag acggcgcgag gtccgtcaga
     8281 aatcgaacac gtagccggcg acgagctgga aatcgggcgc cccgtcctcg aggccgaagc
     8341 cggccgccag gcgaaagaaa ccgtgctcga gcggcttcca gttggctccg acgagaaaca
     8401 gccccgtgtc ctgaccctgc tcgaagcgct tcccctcgaa gtacaactcg gcgacgccga
     8461 cgagctggtc gccgacgggc aacagcgccg cgatcccgag cgagcccgcg atctcgccgg
     8521 cgagctcggt gccgtcgatc gtcgcgtcct cgttgtaacg cagcccgagg ttgccggaca
     8581 gcgtgagccg gcccagctcg tgacgcgccg tcgcgaacag cttcaaccgc gacgcttcgg
     8641 cgctcagtcc cgtgtcggcg tccccggtcg gcagagtcag gacggcaccg ggcgcccagc
     8701 gggtcccggt cttcacactc gatccggcca ggaacttgcc ccagaactcg agatcggtca
     8761 taccgctggc gccgccgcca cggtcgaagt cggtcccggc gtagccgatc cggccaccga
     8821 cctcgagcgt cggcaccgcc cgccagttgt aggccgcgag gaactcggaa acgtagctct
     8881 gcgagcggtc gaaatcggcg acggtgagct gggcctgcgc ccgctgccgg ccgacgaacg
     8941 cggcatcctc gacgaacgag acgaacagtc ggtcggcccc gcggaacgcc cattcgacgc
     9001 gggccggtcg gatcgggtgc tcgagctcga tgtcgaagcg cgtgcccggc agcaccggca
     9061 cgggggtcat ctcggccagc gcgacccgct tgccgagcgg tgaaaccacg gcgatcgggg
     9121 cgcagttgat tccggtgtcg acgtcgaggc gataggatcc ttcgtcgtcg gtcgggggcg
     9181 agacgagaat ccggctcgtc ccggcgacgc gcaggatgac gcgaaagtcg gcgaccggtc
     9241 gctggttcga atcggtcagg atgccggtca gcgggacctt cgcgggttgt gcgaggccgg
     9301 ccccgttccc gacgcacgcg atcgcgcaaa cgagaaggac gcgtcctttc gacaagatca
     9361 tcgctggttc accccgcacg ccgaacgacc ttccggcggc tggtcagtgt accagtatcc
     9421 cgctcggccg cgtagattgt cgctgccgaa ctccgatggt agattgggtt gagagccccg
     9481 ggagagatcg atgatccgtg tgaccgaccg cgccaaggcc aagttcctcg agatcgtccg
     9541 cggcgaagga cgcgagagcg acggcctgcg tgtgatcgtc gagcacggcg gcacggccag
     9601 agccgagttc gcgctcaact tcgtcggacc gggggacggc aacgaagacg acgtggtcga
     9661 ggacgtcgga ccgttcaagg tccacatcga tccgacgagc gcgaagtacc tcgaagaggc
     9721 cagcgtcgac tacgtcgacg ggctcgacga cagcgggttc aaggtcgacg ccccccacgc
     9781 cgggatcgcc ccacccaagg gtccggtcgc cgaagccgtg cgcaaggtgc tggaagagaa
     9841 ggtgaacccc gcggtggcga accacggagg gttcgtcgag ctcgtcgcgg tcgaggacga
     9901 cacggcttat ctgcgcttcg gcggcggatg ccagggttgc ggcatgatca acgtcacgct
     9961 caagcagggt gtggagaaga tcctgttcgc cgaggtggcg ggtatcagca aggtgatgga
    10021 cgtcaccgat catgcggcgg gtaccaaccc ctactaccag ccggccaagt aggcccgcgc
    10081 tgcgccggat cgagatccgt ctcgtttcgt gctacgagct ggggcaccag ccgctggccg
    10141 tcgcgacggc ccaggctgcg ctcgaacggt gcggctttcg tccggcggcc gtcgatctcg
    10201 ccgtcgagcc tctcgcggca gaggcggcga gccggcctgc cgatttgatc gcgatctcgg
    10261 tgccgatgca cacggcgctg cacatcggcg tggccgcggc gcggcgcctg cgaggcgcac
    10321 acgaggcggc gcacatctgt ctgtacgggc tgtacgcctc gctcaacgcc gactacctgc
    10381 tcggcgcggg gctgtgcgac tcgatcgtcg gcggcgagtt cgagcggccg ttggtcgacc
    10441 tggcccgggc gctcgccggt gggtcgcccg tcgaggaggt ccgcggtctg cggctcgccg
    10501 gtcggccgtc gccgccgaac gtggagcgga ccccgttcga cctgccggcg cgcggcggtc
    10561 tgccgccgct cgagcgctac gccaagctgc ggatcgacgg cggggagcgg accgccgccg
    10621 cgatcgaggc cagccgcggc tgtctgcacc actgccgtca ctgtccgatc ccgccggtct
    10681 acgacgggcg ttttttcgtc gtgccgcgcg agctggtgct gggcgacgta cggaacctgg
    10741 tggccgccgg cgtgcgccac ctgaccttcg ccgaccccga cttcctcaac ggcccgcgtc
    10801 acacgatgac gctcgcgcga gagatccacg aagagttccc gcacctgacg ttcgacgtca
    10861 cgacgaagat cgagaacctg ctgcggcacc gcgagcggct gccgcagctc gcgcgtttcg
    10921 gttgcgtctt cgtcgtctcg gcggtcgagt cgctcaacga tacggtgctc gagcacctgg
    10981 cgaaggggca tacccggcgc gacgtcttcg aggcccagcg tctggtcgcc cgggccggca
    11041 tcgcgctgcg gccgtcgctc gtgccgttca cgccgtggga gacgctcgac agctatcgcg
    11101 agctgctcga ctggatcgag tgcgacgcgc tggccgacgg gatcgacccg gtccagcttt
    11161 cgatccgcct gctcgttcca ccgggctcgc tgctcgaacg atcgccggcg atgcggccgt
    11221 acctgcgcgc gttcgagccg cagcggttcg gtcatcgctg ggatcacccc gacccgcgga
    11281 tggatgccct ccaacgggag gtggcgcgca tcgtggcccg cgcggcgcga cacgccgagc
    11341 cgggacgggc gacccacgca cgcatccggc gggcggccgg tgtgcccgcg tcaccggtgc
    11401 cgacccgtgt tccgcccgcg ttggccgccg ccgatccccc acggctgacc gagccgtggt
    11461 tctgttgagc cgagccgctc gaggatccgg gcgatcctca tcgacctctc gggttctaga
    11521 gcccggtgcg ggcggctcag accggctcga tctcgaactt cagcagggcg ccggcggccc
    11581 gcagcgcgtg ctcgaccgct tccggtgtcg aaccgcgggc gaagacgaag ccgagatagc
    11641 gatgaccctc ggggagcggg atgacctcgt ctccgcggtg catcgtcatc gtcacttcct
    11701 cgacgagatc gacgcggcgc gccgcgtcga tgccgcgcac ctcgcgcagg ataccgcgcg
    11761 cgggtatcgg cagcatcatc acgcccgccg cgccgggcac gggatcgagg atcgcgccgt
    11821 cgccgagcgc ctggccgagc accagctgct cgagcgatcg gccgtggccg aaacgcaacg
    11881 tgcgggagca gatcccgccg atcgtgcgcg ccgcgatctc gatcagccac ggacgacctt
    11941 cgctgacgcg caattcggca tgcagcgggc cgtgcaccag gccgagcgcg cgacagccgg
    12001 cgtgcgtctc gtcggcgagc gctcggcgca ccccctcgct cgcccgcgac ggcgtgacga
    12061 gcagcgtctc ctcgaaatac ggtccgacga gggggtcggg cttgtcgaac agcgccagca
    12121 cgtgcagtcg cccacggtcg agcaggccct cgacggcgta ctccgcgccg ggtaggtagc
    12181 gctcgaacag caggtggcgc tggacgccct tcaaccgccg cacttcggca tcgtcgagga
    12241 tggcggcgat gcggcggaac gcgcggacga aaccggaccg atcgtcgacg cgaatcacgc
    12301 cgcggcttcc ggccagaccg accggtttca acacgccggg aaacgacaaa ccgctggcca
    12361 gctcgaccgg gtccacgtcg agcggaaggc gttcgaacgc cggcccgaac agcccggcac
    12421 gcgagaacaa ccggcgcatc tcgtacttgt cacgcgtcgc tcgcacggct tcgatcgggt
    12481 tgtgcggcag gccgagcctg gccgcggcga gcgtcgccaa cagcgtcgtc tcgtcgtcta
    12541 cgccgaggac cgcgtgaaga ccgcggcgcg cggcgagacg ctcgatgtcg tccaacgcgc
    12601 gagcggtgtc gcgcagatcg aaggccagcg tgtgtcccgc cgcgaccgcc tcgagcgcct
    12661 gtcgtcgatc ggtgccgacg atcaactcca cgtccaaccg agcggccgcg cgcatgaagt
    12721 cgtcggtctt gtacgaggtc gtcgggatca gcaacagcac cgaggcaggc atcgcttcat
    12781 cgacccgggg gcggcccgcc cgtcattcgg tcggcacgaa gaccaggatc ggccggtccc
    12841 cgcgcagcag ttggagccgc tcctcgcgta tcgcgaagcc gttgacctgc tcgagatggg
    12901 ccatgtacga ccgctcgagc tccattgcgg gtgcggagca ggctttcatc gtcgacgcca
    12961 gcggaccgaa gccgagcttc ccccgatcga tccggacgcg gccgctgtaa tcgttgcatc
    13021 ccgaatgccc gcggaccgct ccgtcgaggt cgaacgcgat cgtgatgtgg aacccatcgg
    13081 gtatcggtcg ggtcgagatg cccggttcga tccggagcga ccagaccaga ccggccagac
    13141 gcgcgggatc gtcctccgat ccggcgggcg ccgcctcggc ggagcggagc cccggggagg
    13201 gcggcggctc gaccggcagc tccggctgcg ggtcggccgg cgcgggagcg gcgggcggcg
    13261 cggtctcggc ggagccggcc cgcgggcgcg ggtcgccgca cgccgcgacg aacagcacga
    13321 ccgccagcaa cacgatcggc gaccgacgac gggatccctc aacctccggc aacgacgcgg
    13381 ccctccgagg aacctcacga gtatagcgac tgcggcccgg cgcgcacggg tgccgctcgc
    13441 cggcgtgcgg cggccgttcg gcggccgttc cgaccgctcg gccggccgcc tcggccggcc
    13501 ccgcgcgact gccgtatgct tgctggaccc cggcccgcat cccggccgtg cacgagcgac
    13561 gaggaagtcg tggaactgct gcggatcgtt cagttgctga cctacagctt cggcgcctac
    13621 gccttcggat cggcgctggt gatgaccctg cgtccggcga gtccccggcc cgacgactgc
    13681 ctcgcacgag gcccatcgcg tttcgcccga acggcggacc tgctgatgct cgtggtctgc
    13741 ctcgtttggt tctgcacgct gctggcccac acgctggatg agttgcggcc gggcagatgg
    13801 ctgtcgatcg acctgacgcg tctcgtgctg atgtaccagt tcccgccgct gatcgccctc
    13861 gtcaactatg ccgacgcgcg gaagcagacc gaggcgctgg cccacgcggc ctggcgtacc
    13921 acgctcgtcg gcctgtgggt gctggcggag gccacgctgg cctacgccct gctcgcggtc
    13981 tacgacgtgc tgccggcggt ccgaccgctc gacgtctacc tcgggctgtc gatttccgcg
    14041 ctgttcgtgc tcgcctcggt gttcagctcg ttcgtcgtcg tccgcgggcg cccgcaccgc
    14101 gagacccaaa ccgaacgctc gggccggcgc tggatgctcg ggttgtacct gctgatggtc
    14161 gcgctggtcg gcctgatcgt gacggcctac tcgctcggct ggccgatcgc cgaagtcttc
    14221 gcgttcgtcg gcacgtcgct gccgctgatc ttcatgttta cggcgaccta tcacgccgat
    14281 cgcttcgagt ttttcgacct gttcgtcaag cgcggtctgt tggtgctggc gacgatcgtc
    14341 ctgctgacgg cgttcttcgg cggcgtgctg ccgcgcctcg aggcgatcga gctgggctgg
    14401 gccaggccgt gggtctacgc cgtcgcgttg tcaccggcgg tgctggcgct gccgtgggtc
    14461 taccgcaaga tgggcgcgtg gctcgacaac gtctggctcg gacgacggct gacgacgctc
    14521 gaggcgatga agcggttcct gtccgatctg ggccagggcg ccgaccggcg cgacctggtg
    14581 ctgcgcgccg agcgcggctt gtccgagatc ttcaatgccc cggcgcaggt gcggctgagc
    14641 ccggaacgag cgcagccgga cgacgccgag tggttgcagc aggctccggt cgtctcgccc
    14701 gacggcacgg tcggggtgat ccgcttgggg cgacgtgcca accacgtccc gtacttcagc
    14761 gaggaccgcg cattgctcgg ctcgctcgcc gacgttttct cccacatgct gcgcgccgtc
    14821 gatctgcacc ggaagaaacg ggagcaagaa cagctcgcgc gcgagctatc cctgcgcgcg
    14881 agccgtgccg agctgcgtgc gctgagggcc cagatcaatc cccacttcct gttcaatgcg
    14941 ctcaacacga tcgcggcgtt gatccaccag gatcccgcgc gcgccgacca aacggtcgag
    15001 cagctcgccg agatctttcg ctacacgctg cgcgggtcag acagcgagtg gaccccgctc
    15061 gacgacgaga tcgatttcgt ccgatcctac ctcgaggtcg agcgcgcccg attcggcgag
    15121 cgactgagcg tggagatcac ggcggacgcg gaggcacgcg cgcacttcgt gccgacgatg
    15181 atgatccaga cgctggtcga gaacgcggtc aagcacggcg tggcgcgcgt ccgcggcgtg
    15241 gcccggatcg aggtcgacgc gacggtcgcc ggcgaccggc tcgtcgtcgc cgtgcgcgac
    15301 agcgggccgg gcttcgaccg ggcgactccg gcgtcggtca agcgcggcgc cggtggcggc
    15361 ggctacggac tgcacaacgt ccgccggcgg ctcctcggtc acttcggcgc tcaggcggcg
    15421 ctcgaggtgc ggcgcgacga gacccgcggg cgcaccgagg tccgactggt ctttccgtcc
    15481 gcgatgttgg aacgcgttcc gcgaccggat gcgggccgtt ccggcccgga gacccaggga
    15541 gcggcgcgat gacgatccgc gcactgctcg tcgacgacga acccccggcg cgggcccggc
    15601 tgcgcgcgat gctcgaccgc atcgacggtc tgcagatcgt cggcgaggcc agcaacggtg
    15661 tcgaagcggt cgagcagatc ggtgccacgc ggccggatct ggtcttcctc gacgtcgaga
    15721 tgcccgggtt gagcgggata caggtcgcgg cgagcctcgc cccaccgcgc cccgcgatcg
    15781 tcttctgcac ggcctacgac cgctacgcga tccccgcctt cgaccatcat gcgaccgatt
    15841 acgtgctgaa accggtccag gccgccaggc tcgaacgaac gatcgcgcgg gtgcgcgaga
    15901 cactcgcgca cgaggcgacc ctgcgcggag agctggaggc cgccaccgat acgcagcggc
    15961 ggctgctgcc ccagcgcctg ccggagatgc ggacgctcga ctacgccggc agctgccgac
    16021 cggccaagga ggtcggcggc gactactacg acttcctggc gctcgacgac ggcggattgg
    16081 gcttggcgct cgccgacgtg tcggggaagg gtatcttcgc ggcgctgctc tcggccagcc
    16141 tgcaggccaa ggtgcaggcg ttggccccgc actacgagcg cgtggatcag ctggtcggtc
    16201 agatcaaccg catgacccac gcgtcgatcg cggccaaccg ctacgcgacg atgttctacg
    16261 gtcgctacga cgacgagacg cgggcgctcg actacgtcaa cgccggccac ctgccgccga
    16321 tcgtcgtacg ccgtggcgcg aggccggggg agacccggat cgagcgcctg gcgccgagcg
    16381 ggacggtggt cgggctgctg gccgaggcgc gctaccggcg gcggcgaatc gcgctgcgac
    16441 cgggtgatct gttctgcatc ttcaccgacg ggctgagcga gacgcgcgac gcggccggcg
    16501 aggagttcgg ctgcacacgg ctcgagcggc tgttgaccgc gcattccgcc gagagcgacg
    16561 ccgagcgggt gacgagcgcg atccgcgacg agctcgacgc cttcgccggg gcccgtacgc
    16621 gggaggacga cttgacctgg atcgtcgggt gtgtgcggtg agtgaccgcc cgctcagcgt
    16681 gctgatcgtc gacgacgagc gaccggcgcg cgaacggctc gaacggctgc tcgcggggga
    16741 tcgccggctc gcggtgacgg ggcacgccga ggacggcgag caggcgatgg accagatcgt
    16801 cggattgcgc cccgacatcg tgttcctcga catccagatg ccgggttg
//
LOCUS       DASPGN010000413         4667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1596964, whole genome shotgun sequence.
ACCESSION   DASPGN010000413 DASPGN010000000
VERSION     DASPGN010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1596964"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /gene="arsM"
                     /locus_tag="VD788_12000"
     CDS             <1..661
                     /gene="arsM"
                     /locus_tag="VD788_12000"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HXV77029.1"
                     /translation="AYLGLGCGNPQAIAAIREGETVLDLGAGGGFDCFLAAKRVGPAG
                     HVIGVDMTAEMVSTARANAAKLGTTNVEFRLGEIERLPVADGTIDVILSNCVVNLSPD
                     KAAVFREAFRVLKPGGRLAIADVVATAPLPDALKGDVAALTGCIAGAAEVVTLERLLA
                     DAGFDDVQIRVHPESREFIRDWMPGAGVERFVASASIEARKPAGAACCGPSCCAAGDP
                     A"
     gene            658..1257
                     /locus_tag="VD788_12005"
     CDS             658..1257
                     /locus_tag="VD788_12005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV77030.1"
                     /translation="MIESTTPRDLTGDRAWRELGAELRPFVARRVRSAADVDDVLQEI
                     FVRIQRGLVSLRDEERFGPWVYRLARSAVVDHLRSTSRHARPGGPGGQEPGTDPDDAD
                     RGEAEREMAGYVALFLAMLPSPYREALTLTELEGMTQKEAAEMLGISVSGMKSRVQRG
                     RRKLRRALEDCCRIALDARHRVIDYRPREDGRPPDGCCG"
     gene            1312..2379
                     /locus_tag="VD788_12010"
     CDS             1312..2379
                     /locus_tag="VD788_12010"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HXV77031.1"
                     /translation="MDPRIDRPRGRGATRNVRSRFERLGYEPFHDDGADPPADPRTTL
                     IADRSRSIITRNDSPDIPFDRSINPYRGCEHGCSYCFARPTHAYLDMSPGLDFETKIV
                     FKPNAPELLERELARRDYACLPLALGTNTDPYQPAERGLELTRRLLLVLERHRHPVTI
                     VTKSALVTRDLDILAPMAERRLAAVYLSITTLDDQLARRMEPRAAAPARRLATLARLA
                     DHGVPTGVLASPIIPGLNDDELERILEAAAKHGASRAGYLLLRLPHEVREIFSDWLER
                     AYPGRASKILARIREMRGGELNDPRYGERHVGRGPFAELLARRFELAVRRLGLADRRT
                     ELDTTQFRVPRGGGRQGALFD"
     gene            2381..3076
                     /locus_tag="VD788_12015"
     CDS             2381..3076
                     /locus_tag="VD788_12015"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HXV77032.1"
                     /translation="MRRSVPADYTARMLPAESIERWLRDAFRAPLPGPAAQNRMAPRP
                     RTGWQPGVVPDGCRPGAGLLLVYPVDGLPVVLLTKRDPLLAQHAGQVSLPGGALDPDE
                     TVEQAALREAFEEVGLVSHGIRVIGQLTPLHIPVSQFVLHPVVACSERRPELVARPGE
                     VERLLEPALGCLADSRNYAIERRLFRGQEFRVPYVSVGGEKLWGATAMILAEFLTLLG
                     SPPDPWVTDQRRA"
     gene            complement(3061..3291)
                     /locus_tag="VD788_12020"
     CDS             complement(3061..3291)
                     /locus_tag="VD788_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77033.1"
                     /translation="MKRVRIVSLVLLGLIALLLVVLAVRNPAPPFLPADADHAAAGDT
                     PACLACHGVDAPMPRSTNHPLGEDCWRCHARR"
     gene            3412..4152
                     /locus_tag="VD788_12025"
     CDS             3412..4152
                     /locus_tag="VD788_12025"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXV77034.1"
                     /translation="MWSRRPNKVLIVARDPAAAGLSPAFLQHSGIDLHHVAGGSAALD
                     AARRELPRLIVQQVDAPPDEAIALARGLKNDPHTAAIPLIAVADERLGDELTAAGVDA
                     IVNRPLVLREYWDAVRRFVHLPRRRALRESINLRFTYVESERRSQAFSRDLSPYGAFL
                     KTDQHVSVGARITVRFNLPGDPVAIECSAIVRGTCYADAHTAGFAIEFEGMAETDVAR
                     LERFVDRSQQRGLFGVLLAVVVPGERPG"
     gene            4190..4510
                     /locus_tag="VD788_12030"
     CDS             4190..4510
                     /locus_tag="VD788_12030"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HXV77035.1"
                     /translation="MSRDDQDDDGRTEGSGNRILVRTWNDGEALMVRQLLATYGIPCQ
                     VVSDVPHTVLPLTIDGLGEIRILVPAARLRAARGVLADHLRHGLEVLDGGRPDGDDED
                     EDVA"
BASE COUNT          672 a         1672 c         1642 g          681 t
ORIGIN      
        1 cgcctacctc ggtctcggct gcggcaaccc tcaggcgatc gccgcgatcc gggaagggga
       61 gaccgtgctc gacctgggcg ccggcggcgg cttcgattgc ttcctcgccg ccaagcgggt
      121 gggtcccgcc ggccacgtca tcggcgtcga catgacggcg gagatggtat cgacggcgcg
      181 cgccaacgcg gcgaagctcg gcacgacgaa cgtcgagttc cgcctcggcg agatcgagcg
      241 cctgccggtg gccgatggca cgatcgacgt gatcctgtcg aactgcgtcg tcaacctgag
      301 ccccgacaag gcggcggtct tccgcgaggc gtttcgcgtg ctcaagccgg gcggacgcct
      361 cgcgatcgcg gacgtcgtcg cgacggcgcc gcttcccgac gccctcaaag gcgacgtggc
      421 cgcgctgacc ggttgtatcg cgggggctgc cgaagtcgtg acgctcgagc ggttgctcgc
      481 cgatgccggc ttcgacgacg tgcagatccg cgtccatccc gaaagccgcg agttcatccg
      541 tgactggatg ccgggcgcgg gcgtggagcg cttcgtcgcc tcggcgagca tcgaggcgcg
      601 gaagccggcg ggcgcggctt gctgcgggcc gtcgtgctgc gcggcggggg atccggcatg
      661 atcgagtcga cgacccctcg cgatctcacc ggcgaccggg cgtggcgcga gctcggagcc
      721 gagctgcggc cgttcgtcgc gaggcgcgtg aggagcgcgg ccgacgtcga cgacgtgctg
      781 caggagatct tcgtccggat ccagcgcggg ctcgtctcgc tgcgcgacga ggagcgcttc
      841 gggccgtggg tctaccgcct cgcgcggagc gcggtcgtcg atcacctgcg gtcgacctcg
      901 cgccacgcgc ggccgggcgg gcccggtggg caggagccgg gcacggaccc ggacgatgcg
      961 gaccgcggcg aagcggagcg ggagatggcg ggctacgtcg cgctcttcct cgcgatgctg
     1021 ccgtcgccct accgcgaggc gctgacgctg accgagctcg aagggatgac gcagaaggaa
     1081 gcggccgaga tgctcggtat ctcggtctcc gggatgaaat cgcgggtcca gcgcggccgc
     1141 cgcaagctgc gccgagcgct cgaggattgc tgccggatcg cactcgacgc gcgccaccgc
     1201 gtgatcgact accgaccgcg cgaggacggc aggccgcccg acggctgctg cggctgacgc
     1261 acacgtattt tcgtcttgct ttcgtctccg gggcgcggca tgatgcccgg gatggacccg
     1321 aggatcgacc ggccacgcgg ccggggtgcg acgcgcaacg tgcggagccg tttcgagcgg
     1381 ctgggctacg agccctttca cgacgacggc gccgaccccc ccgccgaccc gcgcaccacg
     1441 ctgatcgccg atcggtcccg ctcgatcatc acgcgcaacg attcgcccga catcccgttc
     1501 gaccgctcga tcaacccgta ccgcggatgc gagcacggct gcagctactg cttcgcccgc
     1561 ccgacccacg cctacctcga catgtcgccc gggctcgact tcgagacgaa gatcgtgttc
     1621 aagccgaacg cacccgagct gctcgaacgc gagctggctc ggcgcgacta cgcctgcctc
     1681 ccgctggcgc tcggcacgaa caccgacccg taccaaccgg ccgaacgcgg actcgagctg
     1741 acgcgccggc tcctgctcgt gctcgagcgc caccggcacc cggtcacgat cgtgaccaaa
     1801 tcggcgctcg tcacgcgcga tctcgacatc ctggcgccga tggccgagcg acggctcgcc
     1861 gcggtctatc tctcgatcac gacgctcgac gaccagctcg cgcgccggat ggagccacgg
     1921 gccgccgccc ctgcgcgccg gctcgcaacg ctcgcgcgac tcgccgacca cggcgtcccg
     1981 accggcgtgt tggcctcgcc gatcatcccc ggcttgaacg acgacgagct cgagcggatc
     2041 ctcgaggccg ccgcgaagca tggcgcgagc cgggcgggct acctgctgct gcgcctgccg
     2101 cacgaggtgc gcgagatctt cagcgactgg ctcgagcggg cctacccggg tcgtgcgagc
     2161 aaaatactcg cccggatccg cgagatgcgc ggtggggagc tgaacgaccc gcgttacggc
     2221 gagcgccacg tcggccgggg tccgttcgcc gagctgctcg cccggcggtt cgagctggcc
     2281 gtgcgccgtc tcggtctcgc cgaccggcga accgagctcg acacgaccca gttccgcgtg
     2341 ccgcgcggcg gcgggcgcca gggcgctctg ttcgactgac gtgcgccgtt cggtgccggc
     2401 ggactacact gcgcggatgc tgccggcgga atcgatcgag cgttggctgc gcgatgcgtt
     2461 ccgcgctccg ctgcccggtc ccgcggcgca aaaccggatg gctccgcgcc cgcgcacggg
     2521 ctggcaaccg ggcgtggtcc ccgacggctg ccgtccgggc gccggtctgc tgctcgtcta
     2581 cccggtcgac ggcctcccgg tcgtgctctt gaccaagcgc gatcccctgc tggcgcagca
     2641 tgccggccag gtttcccttc ccggcggagc gctcgacccc gacgagacgg tcgagcaggc
     2701 cgcgctgcgc gaggctttcg aggaggtcgg tctcgtgtcg cacgggatcc gcgtcatcgg
     2761 tcaactgacc ccgctgcaca tcccggtgag ccagttcgtc ctgcaccccg tcgtcgcctg
     2821 ctctgagcgg cgcccggagc tcgtcgcacg acccggggag gtcgaacgcc tcctcgagcc
     2881 ggcgttgggc tgcctcgccg acagccggaa ctacgccatc gagcggcgcc tcttccgtgg
     2941 tcaggagttt cgggttccgt atgtgtcggt cggcggcgag aagctgtggg gcgcgacggc
     3001 gatgattctc gccgagttcc tcaccctgct cggatcgccg ccggatccgt gggtcaccga
     3061 tcaacgacgg gcgtgacagc gccagcagtc ctccccgagc gggtggttcg tcgagcgcgg
     3121 catcggggcg tcgacgccgt gacaggcgag acaggccggc gtgtcgccgg ccgccgcgtg
     3181 atcggcatcc gctggcaaga agggcggggc cgggttgcgc accgccagca cgaccaacag
     3241 cagcgcgatc aggccgagca gtacgagcga gacgatccgt acgcgtttca tcaccgacga
     3301 ctctgaccga ttgcgggcgt tcccgcaacg gaacgagcac gttgacgcgc ggcacgccgc
     3361 agcctagatt ccactcggcc ggggccgtgc ggaggtagag ccgtcaactc gatgtggagt
     3421 cggcgtccca acaaggtgct gatcgtggcg cgcgacccgg cggcggccgg tctgagccct
     3481 gccttcctgc agcactccgg catcgacctg caccacgtcg ccggcggttc ggcggctctc
     3541 gacgccgcgc ggcgcgagct gccgcgactg atcgtccagc aggtcgacgc cccgccggac
     3601 gaggcgatcg cgctcgcccg gggcctgaag aacgatccgc ataccgccgc gatcccgttg
     3661 atcgccgtcg ccgacgagcg gctcggcgac gagctgaccg ccgccggagt cgacgcgatc
     3721 gtcaaccggc cgctcgtgct gcgcgagtac tgggacgcgg tgcgacgatt cgtccatctg
     3781 ccgcgccgcc gcgccctgcg cgagtcgatc aacctgcgct tcacctacgt cgagtccgag
     3841 cgccgatcgc aggccttcag ccgcgacctg tcgccctacg gcgctttcct caagacggac
     3901 cagcacgtga gcgtgggcgc gcggatcacg gtgcggttca acctcccggg cgaccccgtc
     3961 gcgatcgagt gctccgcgat cgtccgcggc acgtgctacg ccgacgcgca taccgcgggc
     4021 ttcgccatcg agttcgaggg gatggccgaa acggacgtcg cccgactcga gcgcttcgtc
     4081 gaccgctcgc agcagcgcgg gctgttcggc gtcctgctgg ccgtcgttgt cccgggggaa
     4141 agaccgggat agactgaacc gaacggcacc ccccgcgagg tgaggatcgg tgagtcgaga
     4201 cgaccaagac gacgacggcc ggaccgaggg aagcggcaac cggatcctgg ttcgaacctg
     4261 gaacgacggc gaggcgttga tggtacgcca gctgctcgcg acttacggga tcccgtgcca
     4321 ggtcgtatcg gacgtgccgc atacggtcct accgttgacg atcgacggct tgggggagat
     4381 ccgcatcctg gttcccgcgg cacggctgcg cgccgcacgc ggcgtgctcg ccgaccattt
     4441 gcgccacggt ctggaagtgc tcgacggagg ccgcccggac ggcgacgacg aagacgaaga
     4501 cgtcgcctga tcggtcgcgc tagcgcacgt cgagcgggat cttgagccgc tcggttttcg
     4561 gggcgcagaa cccgctcttg acgacgcagt aataaaaggt cacctcgccg tcgacctcgt
     4621 gacgaccctc gggcaccgcg ccgtcgagcg ccaggcgcac ggtgatc
//
LOCUS       DASPGN010000414         9552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1129119, whole genome shotgun sequence.
ACCESSION   DASPGN010000414 DASPGN010000000
VERSION     DASPGN010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9552
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1129119"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /gene="speA"
                     /locus_tag="VD788_12035"
     CDS             complement(<1..1290)
                     /gene="speA"
                     /locus_tag="VD788_12035"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HXV77036.1"
                     /translation="MMKDGEGTSMGVTRSRPRWKLSEAEELYQVNAWGDGYFAINRDG
                     HLEVRPDRRKQGCDLYRLVESLEQRGLTPPILLRFDQIVQDRVRRIQQAFHRVIEDSS
                     YEGTYHLAYPIKVNQQYQVVEAVRTAGSNGPLALEVGSKPELLAVMAIHDIDGALLIC
                     NGYKDSEYIELALLAKKLGRRPVVVIEQLDEVRLVLQISEKLDTEIELGIRMKPSTKG
                     AGRWDESAGERAKFGLSTWDVLKVAEQLAAVGKRDWLKLLHLHVGSQITSIGAITRVL
                     REATRVYTEVAKLCPALCFFDAGGGLGVDYDGSRTSFHSSMNYSVAEYAEEVVYAILE
                     RCKETGVPHPDIITESGRATVAHHAMLVVEATDTSRARVPLPEDGTPGTDHEVVHYLR
                     ELHAGLTLKNCRETLHEALVMRDEMLEQFIRGDLSLVA"
     gene            1432..2055
                     /locus_tag="VD788_12040"
     CDS             1432..2055
                     /locus_tag="VD788_12040"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXV77037.1"
                     /translation="MSRNSSSKQRGAIKTGCAVAAGVVVLLVVIVALTTVGRYNNLVT
                     LQESVEAAWSEIDNQYKRRYDLIPQLVSTVQGAADFEKSTLEAVTEARASVGRAQLQS
                     YVEAQQGLSAALGRLFVVVERYPDIKANQNFLSLQDQLEGTENRIAVARRDYIDRVRQ
                     YNTYRRRFPSNLVAGLLGFDAQPQLEIDAGERETPQVEFDFGSDDGS"
     gene            2055..2816
                     /locus_tag="VD788_12045"
     CDS             2055..2816
                     /locus_tag="VD788_12045"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXV77038.1"
                     /translation="MRRAVLHCGLVPAAVWAAAFTVAAQGVELPANDGWVTDLAGFLT
                     PEEERSLETQMESYRQGSGNEIALLTVAELGGQTIERYALEVGRSWGMGQRDRHNAAL
                     LVVARDERKVRIEVARGLEGNLTDSISGRIIRNVIAPPFKQGRYYDGLRAGVAAMQAA
                     AGGDYAAIPESRGSRGSGIAGLIGPILVILIVLAILRGGRRGGGLGGALPWLIAGSAL
                     GGRSHSRGWHSGGGGGFGGFGGGGGFSGGGASGGW"
     gene            2816..3586
                     /locus_tag="VD788_12050"
     CDS             2816..3586
                     /locus_tag="VD788_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXV77039.1"
                     /translation="MAAVWTAVGEQAGGGAWYAIVVALPLAFLALLLFLVLLAASRQR
                     RYRVAAGFGDDDRAAVRAALAAAERRTVGEILPVVVERSDPHPGAEWLAALVLVLLGS
                     SALLAWMPWQHPVVVLALQLLFGGLGFGLARLLPDFKRRFVLERRADEVSREQAFQEF
                     YANGLHRTTAATGVLIFVSLFERRVVILADEGIDAVVDASFWADTDDRVLDGIRRGSL
                     RDGLIAGIARAGELLAARFPWTEGDRNEIPDRLIVRRE"
     gene            complement(3594..4460)
                     /locus_tag="VD788_12055"
     CDS             complement(3594..4460)
                     /locus_tag="VD788_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77040.1"
                     /translation="MQRATAARKVLPLLSLFVVAATGCGPGDDHPHPHPEEEETARAA
                     AAEAQAATRRSEKIRSAVDDPLRPDEDRARDADRKPAEVLAFFGIDEGMRVADLQAST
                     GYYTEILSSVVGPDGEVYAQNNAFVTDRFGEPLGLRLAKLQQAGRDNVVRITAEVDEI
                     ELPPDLDAALFVRFYHDLFWLPKPDGSRADRAEFLRRVHAALRPGGVFGVIDHHAEAG
                     SLERDALERDGLHRIDRELVLAEIEAAGFVLDGESDLLANPADTRDWNIFSEGRRDKT
                     DRFVLRFVKPAS"
     gene            4748..5134
                     /locus_tag="VD788_12060"
     CDS             4748..5134
                     /locus_tag="VD788_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77041.1"
                     /translation="MTHNLRSRLIFFVAIAIAGAGPAGAAAPADGKVVLEADRGGRPA
                     APDADRVIRLQRALEPIFGATRVSSTEAVVATAGTIRLVVDERHAVFSIARIDGEGRL
                     TTECVTGAAEAASRMDEADDGAVSQE"
     gene            5137..6846
                     /locus_tag="VD788_12065"
     CDS             5137..6846
                     /locus_tag="VD788_12065"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HXV77042.1"
                     /translation="MPRPRRSPVRWTSALALAAGLAAAGPALRAEASIVLNVVDPDGV
                     GFNDPTPVDPVPQNPGTTLGEQRRIVFERAAELWGSILDSDSEIVVQAAFRPLPCTAG
                     SGLLASAGSVRILANFPGAPFDDTWYQPALANKLAGYDLTPGPPDPGLLADPFNDDLY
                     LVLNLSVDDDPQCLGGAGWYYGLDNRAGSRVDLINVLMHELAHGFGFANFVDETTGAG
                     PAGYTDAFSRHTLDNTTGLHWHEMTDAQRSASATRSGEVVWDGPHVRAEAPHVLGPAP
                     VLEVIAPAEGAGTYEVTTATFGAEVDPDGVPGELVLAFDAVGTASDACEELVGDYTGA
                     IVVVDRGSCSFTDKAARVQQAGGAGIVVVNDRPVGLPPMTGQDETIAIPAVGLSAADG
                     AHVREAMPGATGVIGFDESRLAGTDAARNVRLYAEPAGSSAISHWDLTASPSLLMEPV
                     FHPDLESTETVDLTSRALADLGWDACPDSDFSPDVWIGDCDPGVANRVMDDGCTIVDV
                     LAECALGASLDRKCLTDKTKLLVDGKVLESRDRAAIRQCATGNGRPPRGERPTGATRR
                     TPR"
     gene            6903..8198
                     /locus_tag="VD788_12070"
     CDS             6903..8198
                     /locus_tag="VD788_12070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV77043.1"
                     /translation="MTDGSEWVDECDVDERTRDEILRLRRRVTELETELETEHAEFRR
                     STHGTQDQREILESIVSQLGDGVVVADQTGKFLLFNPAAERIVGIGITDAPPERWSEV
                     YGIYYPDRVTPFPWSDLPLVRALEGHETRDVEMFVRHATLPDGVFISVSGTPLMSGDT
                     VRGAVVVFRDVSQSRNAGEARRLLELAHHQADERRRVEAELERVRDTLIRRTRFSAIG
                     ELAASIAHDLRNPLGAIRNSSFYLSRQVPPDHADWSRHLEIIEHETRAADRIIDNLLD
                     MSRSKAPEREELDLAVVVREAFGGDHGPTGVELELRLEGGPYRVWADHSQLRRLLGNL
                     LTNAEQAMDGTGKVVVRARRVGEMDVITITDQGKGVPDALRERIFEPLFTTRAKGTGL
                     GLAICRQIAERHDGTLALSETGEAGSTFELKLPHAAGID"
     gene            complement(8208..8876)
                     /locus_tag="VD788_12075"
     CDS             complement(8208..8876)
                     /locus_tag="VD788_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77044.1"
                     /translation="MTGARATVWTVLVPLLCLGAGASTEDPAAGVEARVAALTAGLEL
                     TDEQRPVVERALEQMIRRQRHAVSQLAAAADPTAPPGLGALADELRAAEREAREAMDD
                     VLTEAQLSAVARAVADERARAAGEAIAGRLRQPLELTDEQYDRLVPVFTEHVERRAAQ
                     RNAPGRSFGAVRNAREAARRMQVELESDLEPILTPEQMERYRELVEQARERLRGRERG
                     TRGR"
     gene            complement(8873..9331)
                     /locus_tag="VD788_12080"
     CDS             complement(8873..9331)
                     /locus_tag="VD788_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77045.1"
                     /translation="MSDPVDSLAVDDRARRRGVTLMLVVFVLGLVCGAALSIITVRSV
                     LPRPVARPAFDDRPGDGRPVAGPGARFERMAERLDLDEEQRVRVREILRRSRQEIRDV
                     FETSRQEIIEVLDEEQREEFERMRTSEQRRRRPGGRRGHPDPPPDDGRTQ"
     gene            complement(9328..>9552)
                     /locus_tag="VD788_12085"
     CDS             complement(9328..>9552)
                     /locus_tag="VD788_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77046.1"
                     /translation="ASALVASIPYARRAIPRLAAAAALLVILSAVLSWGGRAAPRATA
                     NESFAELLIAGSELGGDPWFESLTGAEDSR"
BASE COUNT         1330 a         3382 c         3420 g         1420 t
ORIGIN      
        1 cgcgacgagg ctcagatcgc cgcggatgaa ctgctcgagc atctcgtcgc gcatcaccag
       61 cgcctcgtgc agcgtctcgc ggcagttctt cagcgtcagc ccggcgtgca gctcgcgcag
      121 gtagtggacg acctcgtggt cggtgccggg cgtgccgtcc tccggcagcg gcacgcgggc
      181 ccgcgacgtg tcggtcgcct cgaccaccag catcgcgtgg tgggccaccg tcgcccggcc
      241 cgactcggtg atgatgtcgg ggtgcgggac gcccgtttcc ttgcaacgct cgaggatcgc
      301 gtagaccacc tcctcggcgt actcggcgac cgagtagttc atcgacgagt ggaagctggt
      361 gcgcgagccg tcgtagtcga cgcccaagcc gccgccggcg tcgaagaagc agagcgccgg
      421 acagagcttg gccacctcgg tgtagacacg cgtcgcctcg cgcagcacgc gggtgatggc
      481 gccgatcgag gtgatctgac tgccgacgtg cagatgcagc agcttgagcc agtcgcgctt
      541 gccgacggcg gcgagctgct cggcgacctt gagcacgtcc cacgtgctca gaccgaactt
      601 ggcgcgctcc ccggccgact cgtcccagcg accggcgccc ttggtgctgg gcttcatccg
      661 gatgccgagc tcgatctcgg tgtcgagctt ctccgagatc tgcagcacca ggcgcacctc
      721 gtcgagctgc tcgatgacga cgaccggccg gcgccccagc ttcttcgcca acagcgccag
      781 ctcgatgtac tcgctgtcct tgtacccatt gcagatcagt agcgcgccgt cgatgtcgtg
      841 gatcgccatc accgccaaga gctcgggctt gctcccgacc tcgagcgcca gcggcccgtt
      901 gctgccggcc gttctcaccg cctcgacgac ctggtactgc tggttgacct tgatcgggta
      961 ggccaggtgg taggtgccct cgtagctcga gtcctcgatc acccggtgga aggcctgctg
     1021 aatccggcgg acgcgatcct gcacgatctg gtcgaaccgc aacaggatcg gcggggtcag
     1081 cccgcgctgc tccaggctct cgaccaggcg gtagaggtcg cagccctgtt tgcgccggtc
     1141 gggacgcacc tcgaggtgcc cgtcgcggtt gatggcgaag tagccgtcgc cccaggcgtt
     1201 gacctggtag agctcttcgg cctccgacag cttccagcgc ggtcggctcc tcgttacgcc
     1261 catcgacgtg ccctctccgt ccttcatcat cggccgtgcc ctcgcagaga ttagcagatc
     1321 cgagcggggc cgtgcgcggc ccgtttcacg cggacccggc ccggcgctcg ccgcggatcg
     1381 ggttgacgac cgtgaaagcg aggcggtagc ctgggcgtcg gagggcaagg aatgtcgcgc
     1441 aacagctcga gcaagcagag aggggcgatc aagaccggct gcgccgtcgc cgccggcgtg
     1501 gtcgtgctgc tggtcgtgat cgtcgcgctg acgacggtcg gccgctacaa caacctggtg
     1561 acgttgcagg agagcgtcga ggccgcctgg agcgagatcg acaaccagta caaacgccgc
     1621 tacgacctga tcccgcagct ggtgtcgacg gtccagggcg ccgccgactt cgagaaatcg
     1681 acgctcgagg cggtgaccga ggcgcgggcc agcgtcggcc gggcccagct gcagtcctac
     1741 gtcgaggcgc aacagggcct gtccgccgcg ctcggccggt tgttcgtcgt cgtcgagcgc
     1801 taccccgaca tcaaggccaa ccagaacttt ctctcgctgc aggatcagct cgagggcacc
     1861 gagaaccgca tcgcggtcgc gcggcgcgac tacatcgacc gcgtccgcca gtacaacacc
     1921 taccggcggc gcttcccgag caacctggtc gccggcctgc tcggcttcga cgcgcagccg
     1981 cagctcgaga tcgatgcggg cgagcgcgag acgcctcagg tcgagttcga cttcggcagc
     2041 gacgacggct cgtagtgcga cgagcggtcc tccactgcgg gctcgtgccg gcggccgtct
     2101 gggccgccgc cttcaccgtg gcggcgcagg gagtcgagct gccggcgaac gacggctggg
     2161 tcaccgacct ggccggcttc ctcacacccg aggaggagcg ttcgctcgag acgcagatgg
     2221 agtcgtacag gcagggaagc ggcaacgaga tcgcgttgtt gaccgtggcg gagctcggcg
     2281 ggcagacgat cgagcgctac gcgctcgagg tcggccggag ctggggcatg ggccaacgcg
     2341 accgccacaa cgccgcgttg ctcgtcgtcg cgcgcgacga gcgcaaggtc cgcatcgagg
     2401 tggcgcgcgg tctcgagggt aacctgaccg actcgatctc ggggcggatc atccgcaacg
     2461 tcatcgcgcc gccgttcaag caggggcgct actacgatgg gctgcgcgcg ggcgtcgcgg
     2521 cgatgcaggc cgcggcgggc ggcgactacg ccgcgattcc cgaatcgcgc gggtcgcgtg
     2581 gctcggggat cgccggcctg atcggaccga tcctcgtcat cctgatcgtc ctcgccatcc
     2641 tgcgcggcgg gcggcgcggc ggcggcctgg gtggcgcgct gccgtggttg atcgcgggct
     2701 ccgcgctcgg cggccggtcg cactcgcgcg ggtggcactc ggggggtggc ggcggcttcg
     2761 gcggcttcgg cgggggcgga ggcttctccg gcggcggcgc ctcggggggc tggtgatggc
     2821 ggcggtgtgg acggcggtcg gcgagcaggc cggcggcggg gcctggtacg cgatcgtcgt
     2881 cgcgttgccg ctcgctttcc tcgcgctgct gctgtttctc gtgctgctcg ccgcgtcgcg
     2941 ccagcgccgg tatcgcgtcg ccgccggctt cggcgacgac gatcgcgcgg cggtgcgcgc
     3001 ggcgctggcc gccgccgagc ggcgcacggt cggcgagatc ctgccggtcg tcgtcgagcg
     3061 ctccgacccg cacccggggg ccgagtggtt ggccgcgctc gtcctcgtgc tgctcggctc
     3121 gtcggcgttg ctcgcctgga tgccgtggca gcacccggtc gtcgtgctgg ccctacagct
     3181 gctgttcggc gggctcggct tcggcctggc ccggctgctg cccgacttca aacggcgttt
     3241 cgtcctcgag cgacgcgccg acgaagtcag ccgggagcag gcgttccagg agttctacgc
     3301 caacgggctg caccggacca ccgcggccac cggcgtgctg atcttcgtca gcctgttcga
     3361 gcggcgcgtc gtgatcctgg ccgacgaggg gatcgacgcc gtcgtcgatg cgtcgttctg
     3421 ggccgacacc gacgaccgcg tgctcgacgg catccgccgc ggctcgctgc gggacgggct
     3481 gatcgcggga atcgcgcggg ccggcgagct gctggccgcc cggttcccgt ggacggaggg
     3541 cgatcgcaac gagatcccgg atcggttgat cgtccgccgc gaatagggcc cgctcagctc
     3601 gccggcttga cgaagcgcag gacgaaccgg tcggtcttgt cgcggcggcc ctcggagaag
     3661 atgttccagt cgcgcgtgtc ggccgggttg gccagcagat ccgactcgcc gtctaggacg
     3721 aaacccgcgg cttcgatctc ggccagcacc agctcgcggt cgatgcgatg cagcccgtcg
     3781 cgctcgagcg cgtcgcgctc gagcgatccc gcctcggcgt ggtggtcgat cacgccgaac
     3841 acacctcccg gtctcagcgc ggcgtggacc cggcgcagga actccgcgcg gtcggcccgg
     3901 ctgccgtccg gcttggggag ccagaacagg tcgtggtaga agcggacgaa cagggcggcg
     3961 tcgagatccg gcggcagctc gatctcgtcg acctcggcgg tgatgcgcac cacgttgtcc
     4021 cgcccggcct gttgcagctt ggccagccgc aggccgagcg gctcgccgaa gcggtcggtg
     4081 acgaaggcgt tgttctgggc gtagacctcg ccgtccggtc cgacgaccga cgacaggatc
     4141 tccgtgtagt agccggtcga tgcctgcagg tcggcgacgc gcatcccctc gtcgatgccg
     4201 aagaacgcga gcacctcggc cggtttccgg tcggcgtcgc gcgcgcgatc ctcgtccgga
     4261 cgcagcgggt cgtcgacggc cgagcggatc ttctccgacc gccgcgtcgc cgcctgcgct
     4321 tcggcggccg cggcccgcgc ggtctcctct tcttcgggat gggggtgcgg gtggtcgtcg
     4381 cccgggccgc agccggtggc ggcgacgacg aacagggaga gaagcggcaa gaccttcctc
     4441 gcggcggtgg cgcgctgcat cggggctctc ctcggatccg gtcgaggtgg cgaccctagc
     4501 acaagccgct ctcgcccgct gacagccggt taccatcgat ggaccggaag cgttagccga
     4561 aaacggcccg tggtggtttc cgtcgtccgg cgcgatcacc ttcgggacac tcttacgagt
     4621 tcgcgatcgc aagcggccgt cgagtgacgc tcgctgtcgg gattgtcatt gcttttgtat
     4681 tccgcggccc tcatccattg cggtatcccg atgctcgtgc ccgggcgggc acgtcgagag
     4741 agacgcgatg acccacaacc tgcggagccg gctgatcttc ttcgtcgcga tcgcgatcgc
     4801 gggggctggc cccgcgggcg ccgcggcacc cgccgacggg aaggtcgtgc tcgaagccga
     4861 ccgcgggggt cggccggccg caccggatgc ggatcgggtg atccgtctgc agcgcgcgct
     4921 cgagccgatc ttcggggcga cacgggtgag ctcgaccgag gccgtcgtcg cgacggccgg
     4981 gacgatccgt ctcgtggtcg acgagcgtca cgcggtcttt tcgatcgcgc ggatcgatgg
     5041 ggaagggcga ttgaccaccg aatgcgtcac cggggccgcc gaggccgcgt cgcgaatgga
     5101 cgaggccgac gacggtgccg tgagtcagga gtgagcatgc cccgacctcg tcgctcgccg
     5161 gtccgctgga cctccgcgct ggccctcgcc gccggcctgg ccgccgccgg ccccgcgttg
     5221 cgggccgaag cctcgatcgt gctcaacgtc gtcgatcccg acggcgtggg cttcaacgat
     5281 ccgacccccg tcgacccggt gccgcagaac cccgggacga cgctcggcga gcagcgtcgt
     5341 atcgtcttcg agcgggcggc cgaactgtgg gggtcgatcc tcgacagcga cagcgaaatc
     5401 gtggtccagg ccgcattccg cccgctgccg tgcaccgccg ggtccgggct gttggcgagc
     5461 gccggatcgg tgcgcatcct cgccaacttc ccgggagcgc cgttcgacga cacgtggtac
     5521 cagcccgcgc tggccaacaa gctcgccggc tacgacctga cgccgggacc gcccgacccg
     5581 ggcctgctcg ccgacccgtt caacgacgac ctgtacctgg tgctcaacct ctcggtcgac
     5641 gacgatccgc agtgcctcgg tggcgccggc tggtactacg ggctggacaa ccgggccggc
     5701 tcgcgcgtcg atctgatcaa cgtgctgatg cacgagctgg cccacggctt cggcttcgcc
     5761 aacttcgtcg acgagacgac cggcgccggt ccggcgggct acaccgacgc gttcagccgg
     5821 cacacgctgg acaacacgac cggcctgcac tggcacgaga tgaccgacgc ccagcgctcc
     5881 gcctcggcca cccgctcggg tgaggtggtg tgggacgggc cgcacgtgcg ggccgaggcg
     5941 ccgcacgtcc tgggaccggc cccggtgctc gaggtgatcg ccccggccga gggcgccggg
     6001 acgtacgagg tcacgaccgc cacgttcggc gccgaggtcg acccggacgg cgtgccgggc
     6061 gagctcgtcc tcgccttcga cgcggtcggc acggcgagcg acgcctgcga ggagctggtc
     6121 ggcgactaca ccggggcgat cgtggtcgtc gatcgcgggt cgtgcagctt caccgacaag
     6181 gcggcgcgcg tgcagcaggc cggtggtgcg ggcatcgtcg tggtcaacga ccgtccggtc
     6241 ggcctgccgc cgatgaccgg acaggacgag acgatcgcga tcccggcggt cgggctgtcc
     6301 gccgccgacg gggcgcacgt ccgggaggcg atgccgggcg ccacgggcgt gatcggcttc
     6361 gacgagagcc gtctcgccgg aaccgacgcc gcgcgcaacg tccggctgta cgccgagccg
     6421 gccggttcct ccgcgatctc gcactgggac ctcaccgcct cgcccagcct gctgatggag
     6481 ccggtcttcc acccggacct cgaaagcacc gagacggtcg acctgaccag ccgcgcgctg
     6541 gccgacctgg gctgggacgc ctgtcccgat tccgacttct cgcccgacgt gtggatcggc
     6601 gactgcgacc cgggggtcgc caaccgggtg atggacgacg gctgtacgat cgtcgacgtg
     6661 ctcgccgagt gcgccctcgg agcgtcgctc gaccgcaagt gcctgaccga caagacgaag
     6721 ctgctcgtcg acggcaaggt gctcgagagc cgcgaccggg cggcgatccg gcagtgtgcg
     6781 accgggaacg ggcgcccgcc gcgcggagaa cgaccgacgg gagcaacccg gcgaacccct
     6841 cgctagcgcc cggccgacgg gtgtagactt ggcgccgttt ccttcccggc gggtcgacac
     6901 ggatgaccga cggcagcgag tgggtcgacg aatgcgacgt cgacgagcgg actcgggacg
     6961 agatcctgag gctgcggcgg cgtgtgaccg agctcgaaac cgagctcgaa accgagcacg
     7021 ccgagtttcg tcgctccacg catgggacac aggatcagcg cgagatcctc gagtcgatcg
     7081 tctcgcagct cggcgacggc gtggtcgtcg ccgaccagac cggcaagttc ctgctgttca
     7141 accccgccgc ggaacgcatc gtcggcatcg gcatcaccga cgcgccgccc gagcgatggt
     7201 ccgaggtcta cggcatctac tacccggacc gcgtcacgcc gtttccgtgg agcgacctgc
     7261 cgctcgtgcg cgcgctcgag ggtcacgaaa cgcgcgacgt cgagatgttc gtccggcacg
     7321 cgacgctgcc ggacggcgtg ttcatcagcg tcagcggcac gccgttgatg agcggcgata
     7381 cggtgcgcgg ggcggtggtc gtcttccgcg acgtttcgca gtcgcgcaac gcgggggaag
     7441 ccaggcggtt gctcgagctg gcccatcacc aggccgacga gcgccgacgt gtcgaggccg
     7501 agctcgagcg cgtgcgcgac acgctgatcc ggcgcacgcg gttcagcgcg atcggcgagt
     7561 tggcggccag catcgcgcat gacctacgca acccgctcgg cgcgatccgc aactcgagct
     7621 tctacctcag ccgacaggtg ccgccggacc acgcggactg gtcgcgccat ctcgagatca
     7681 tcgagcacga aacgcgggct gccgatcgga tcatcgacaa cctgctcgac atgagccgct
     7741 cgaaggcccc cgaacgggag gagctcgatc tcgccgtcgt ggtgcgcgag gcgttcggcg
     7801 gagaccacgg accgacgggc gtcgagctcg agctgcgctt ggagggcggg ccgtatcggg
     7861 tgtgggccga ccacagccag ctgcggcgcc tgctcggcaa cctgctgacc aacgccgagc
     7921 aggcgatgga cggcacgggc aaggtcgtcg tgcgcgcccg ccgcgtcggc gagatggacg
     7981 tgatcacgat caccgaccag ggcaagggcg tgcccgacgc actgcgcgag cggatcttcg
     8041 agccgctgtt caccacgcgg gccaagggca ccgggctggg tctcgcgatc tgccggcaga
     8101 tcgccgagcg acacgacgga acgctcgcgt tgagcgagac gggcgaggcg ggatcgacct
     8161 tcgagctcaa gttgccgcac gcggcgggga tcgactgagg cttgccgtca gcggccgcgc
     8221 gttccgcgct cccggccgcg cagacgttcg cgcgcctgtt cgaccagctc gcggtagcgc
     8281 tccatctgct ccggcgtcag gatcggctcg aggtcggact cgagctcgac ctgcatgcgc
     8341 cgcgccgcct cgcgcgcgtt gcgcacggcg ccgaacgacc gcccgggcgc gttgcgctgg
     8401 gcggcgcgcc gctcgacgtg ctcggtgaag accggcacca gccggtcgta ctgctcgtcg
     8461 gtcagctcga gtggctgtcg caggcgccct gcgatcgcct cgcccgcggc gcgcgcccgc
     8521 tcgtcggcga cggcccgcgc gacggcggag agctgggctt ccgtcaacac gtcgtccatc
     8581 gcctcacgcg cctcgcgctc ggccgcccgc agctcgtcgg cgagcgcgcc gagcccggga
     8641 ggcgccgtcg ggtcggcggc ggcggcgagc tggctcacgg cgtggcgttg ccgccggatc
     8701 atctgctcga gcgcccgttc gacgaccggt cgctgctcgt cggtcagctc gagccccgcg
     8761 gtgagcgcgg cgacccgcgc ctcgacgccg gctgcgggat cttccgtcga ggcacccgcc
     8821 ccgagacaca acaacggcac cagcacggtc cacaccgtcg cccgagcccc ggtcattgcg
     8881 ttcgtccatc gtccggcggc gggtcgggat gaccccgacg gccgcccgga cgcctacgtc
     8941 tttgctcgga ggtccgcatc cgttcgaact cctcgcgttg ctcctcgtcg agcacttcga
     9001 tgatctcctg tcgcgacgtc tcgaacacgt cgcgaatctc ctgccggctg cgccgcagga
     9061 tctcgcgcac gcggacgcgc tgctcctcgt cgagatcgag ccgctcggcc atccgctcga
     9121 accgcgcgcc gggcccggcg accggtcggc cgtcgccggg tcgatcgtcg aacgcgggtc
     9181 gggcgacggg acggggcagc acgctgcgga ccgtgatgat gctcagcgcg gcaccgcaga
     9241 cgagacccaa cacgaagacg acgagcatca gcgtcacgcc gcgccgccgc gcgcggtcgt
     9301 cgaccgccag ggagtcgacc gggtcgctca ccggctgtcc tccgcgcccg tcaacgattc
     9361 gaaccacggg tcgccgccga gctcgcttcc ggcgatcaag agctcggcga acgactcgtt
     9421 cgccgtcgcc cgaggcgccg cgcgcccgcc ccacgacagc acggccgaca ggatgacgag
     9481 cagcgccgcc gccgccgcca atcgcgggat cgcacggcgc gcgtagggga tgctcgcgac
     9541 cagcgcgctg gc
//
LOCUS       DASPGN010000415         9096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_311200, whole genome shotgun sequence.
ACCESSION   DASPGN010000415 DASPGN010000000
VERSION     DASPGN010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9096
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311200"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VD788_12090"
     CDS             complement(<1..647)
                     /locus_tag="VD788_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77047.1"
                     /translation="MFPAVRAWSIALLLAAPPSAQDRPREPEPLEVEVDERATVRLVL
                     VDTVVVDSAGRTVPDLTIDDFEVFALGRPVEVDTLDVDCPVGAVDDPAPMRLAAKRPS
                     LPATEQPRRIVLAFDYLHLGALEREEAIDQAQHLLKNGAARGDEVMVAALNGGLRIEQ
                     TFTADAETAFLSLRRMQYDVTLWNGNFEHINEYGFVEGLTGLFDVLGTVPGPKAVV"
     gene            complement(710..895)
                     /locus_tag="VD788_12095"
     CDS             complement(710..895)
                     /locus_tag="VD788_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77048.1"
                     /translation="MSTARTDRPLPHSLRKHLRLEKARLRRELPPEAAQRAIEQLERS
                     LRRAGEAKAKPRSDEPS"
     gene            1240..1827
                     /locus_tag="VD788_12100"
     CDS             1240..1827
                     /locus_tag="VD788_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77049.1"
                     /translation="MLPLTLLLAVGCGKAPTTEIEAADETLDRAEAAGAAEYAPESFG
                     AAEQARAELDTELKAQEEKLGVFRSYSKAQELAGAAKVAAEKAEQDAATAREDAREEA
                     AALIADVRASLTETKELLERAPRGKGSEVDLAALQADLTGIENAAAELDRAFADGRYL
                     EAKTGAEAAMAGAEQIKSEILRAMETAKPRTARRS"
     gene            1784..2974
                     /locus_tag="VD788_12105"
     CDS             1784..2974
                     /locus_tag="VD788_12105"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXV77050.1"
                     /translation="MRWRRRSPGRLGAAEPWLLALVGIAAVACGLQAASGRATPPAYS
                     RYAAERALDRARRADAGAHAPWRLRRADAALADARLEFRRHQLRFYLWRDFTGAREKL
                     RRSEAMAVETEGLARQRLAAERESMRRELEHAACVVGLTASLARTFRFGKQTVEGMRA
                     AKRYLAEAEVFARRQRFDRARLLCQRANVEARSARDRMTDALARFVDGDNVRRWTEQR
                     AHAVRWSAAHSRPALVVNKDEATLAVFVSGRLVATYPVEIGLNHLQRKLRAGDEATPE
                     GIYHVTAKKDRGQTRYHRALELSYPNDDDRRRLARLIDQRVLPPGVTSGDLIEIHGEG
                     GRARDWTLGCVSLDNNAMDDLFDRVAVGTPVTIVGGDGDDGRFSTLAGFLASSGVERA
                     CPDD"
     gene            2955..3608
                     /locus_tag="VD788_12110"
     CDS             2955..3608
                     /locus_tag="VD788_12110"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXV77051.1"
                     /translation="MRAPTIDGGTRRRRAGILVAAAAAFAVCYALGTGYDYAPSGVEP
                     TLTAPDAVDADALRENRRLRTALARHTPREVYLVVDRANNRLYLRRGETTLLEALCSS
                     GSGTVLHAPDGRSWRFDTPSGLFRVRSKTANPVWRRPDWAFVEEGLPLPGRPEDRFEY
                     GSLGEYALHFGDGYLIHGTLYERLLGRSVTHGCIRLGRDDLRRVYDAAKLGTPVYIF"
     gene            3608..4276
                     /locus_tag="VD788_12115"
     CDS             3608..4276
                     /locus_tag="VD788_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77052.1"
                     /translation="MSQLGLARPIAAAVLLLSGAAVATAVFGQFGPETLERTNLRLAE
                     QVTLAERERPYLMLDLSAPRLRLMMGGALLREFAVSGAEVGTPTVLFVRRSPPSPFAG
                     RPRTLAGVHPPRRSRPDEVVAPVPGSVVEPPLVPPDPVEAEPAPERFFLRFDDGLSIE
                     VRQERAATGGWRARYTRLRWKLVDMLDASRPGPASRLRLVMPESEAGSLYRSIPQATP
                     LIVS"
     gene            complement(4344..6512)
                     /gene="glgP"
                     /locus_tag="VD788_12120"
     CDS             complement(4344..6512)
                     /gene="glgP"
                     /locus_tag="VD788_12120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HXV77053.1"
                     /translation="MEMQRFRNASGQAGAKDLRRAAQALATNVPSRLAPLARVAYNYF
                     WSWQPDGDRLFRDIDAYRWRLCGQNPVRFLQEALPESLEKASLSPRIVDRVERLRDAL
                     GADLARPAATGFPADRPVAFFCAEFGIHRSMPIYSGGLGCLAGDILKEASDLAIPMVG
                     VGILYRQGYFHQRVDAAGLQHEYWYETDPERRPAVRVTAADGHPIQVTVPIWAEEVAV
                     QVWRVDVGRVPLFLLDTTVDRNTPRQRFISARLYEGNRQVRLAQYALLGVGGMRVLRA
                     LGIEPLVVHLNEGHPALATIEAMRGLMARGASFGQACTEARRQFVFTTHTPVPAGNET
                     YSADEMRAVFPDVAGQLGTEWEELLALGRVHPDRRDEPPGLTPAAIRLSRSINGVSRI
                     HGEVSRAMWRELFPASAVEQVPISHVTNGAHLPSWMSPPMRRLLDRHLDADWSDPDRV
                     RDPATWAAIDAIPDEELWTVRNALAERLVKWVRSQTVTERLTRDEPMDFVEQAARTFD
                     PEVLTLGFARRIATYKRLHLLLRDPPRLLRLLEGSRPLQLLVSGKAHPKDDVAKALLT
                     ELFRLKTDPRVAGRVAFLEDYDLGKASLLVAGCDVWLNLPRPPLEASGTSGIKAAFNG
                     TLNLSVLDGWWAEAYDGTNGFAIEGSPGGGEADDERDAAALFDLLERQVVPLFHERGA
                     DGIPHGWIARVKASLRTICPRFCATRMLDECARRVYRPAD"
     gene            complement(6522..7283)
                     /locus_tag="VD788_12125"
     CDS             complement(6522..7283)
                     /locus_tag="VD788_12125"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV77054.1"
                     /translation="MDDYDPSTYGNSIAEVYDRWYASLGPDAAVACLRELAGGAGPVL
                     ELGIGTGRIALPLSRAGVEVHGIDSSAAMVAKLRSKPGGDRLRVTLGDFADVAVGGRY
                     PLIFVAFNTLFALTSQEDQLRCFRNVAGRLDPRGRFVLDAFVPDLARFERNQRIGLEA
                     HDPDAVRIEASLHDPVRQTVTSRHVVLTEHGAKFYPLEVRYAWPSELDLMARLAGLRL
                     VHRWGDWNRLPFDSRSTQHVSVYERDAQDAGERDG"
     gene            7536..>9096
                     /locus_tag="VD788_12130"
     CDS             7536..>9096
                     /locus_tag="VD788_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77055.1"
                     /translation="MIRRWTPRTIGALVALTVLLAAGPAFAQGLCDSDPELPIVPVDS
                     DITIDTIWTNDNVYVLRDVIYVRGGATLTIEQGTIVRGVPDATTPSVPNNPGTLVITR
                     DSKIYALGTAREPIVFTDCNNDNLRDFRPGLFPYDDLANVLGEVANWGGLIVLGRTYV
                     ANNSAGGPNPAREVLIEGLEANGECSNDPSISCTSNADCGVGNTCDGIGRGFYGNCSA
                     VYDTLGDPCICDDDDSGSLRYLSISHGGFNLSQDNEINGLTLGAVGRETDVSYIEVFQ
                     SKDDGFEFFGGTVSPDHLVVANTGDDGFDTDEGFRGKLQYLLLMQGKPGTDQGDKGAE
                     MDGGNSPDASRPIAIPTIYNATYVGLGAGGGSSNTALHFRDNTGFRYYNSAFLDFRGA
                     AALVEGATDSTFPRDPALGTAANTSGERAITAFPPAGEDACFYAEVPTSATQLELRDN
                     TFWCIGRQEMPGLAGTLPSLGYCSNVGPGTRNCLDDADCGVGNTCVDKPLDYGISSGD
                     VNKVHFDPGWLA"
BASE COUNT         1397 a         3213 c         3188 g         1298 t
ORIGIN      
        1 acgaccgctt tgggaccggg caccgtgccg agcacgtcga acagaccggt cagtccttcg
       61 acgaagccat actcgttgat gtgctcgaaa ttgccgttcc acagggtgac gtcgtactgc
      121 atccggcgca ggctgaggaa ggccgtctcc gcgtcggcgg tgaaggtctg ctcgatgcgc
      181 agaccaccgt tcagcgccgc gaccatcacc tcgtcaccgc gcgccgcgcc gttcttcaac
      241 aaatgctggg cctgatcgat cgcctcctcg cgctcgaggg cgccgagatg caggtagtcg
      301 aaggccagca cgatgcggcg gggttgctcg gtagccggca gcgacggccg ctttgccgcg
      361 aggcgcatcg gcgccggatc gtcgaccgcg ccgacggggc agtcgacgtc gagggtgtcc
      421 acctcgaccg ggcgccccag cgcgaacacc tcgaagtcgt cgatggtcag atcggggacc
      481 gtccgccccg cggagtcgac gacgacggtg tcgacgagaa cgaggcgcac ggtcgcgcgc
      541 tcgtcgacct ccacctcgag cggctccggt tcgcgaggtc gatcctgggc cgacggcggc
      601 gccgcgagca gcaacgcgat cgaccatgcg cggaccgcgg ggaacattcc gaaccgaccc
      661 cacccggccc acgagcctac cacaggcctg ctcgcgcccg gcgaatgcgt cagctgggct
      721 cgtcgctgcg aggcttcgcc ttggcctcgc cggcccgccg cagactgcgc tcgagttgct
      781 cgatcgcgcg ttgggcggcc tcgggcggaa gctcgcgtcg cagacgggcc ttttccagtc
      841 tcaggtgttt gcgcagcgag tgcggcagcg gacgatcggt acgggcggtg ctcattccgg
      901 tttccctcgc cgccgtacga tgcaatccgg gtgccgcctc cggcggcgat gccggagcaa
      961 gcgacccggc gcggatttcg ccgcctatcg gacccgccgg gtccggtccc gggatcgata
     1021 cggaactgcc gattccgccc accgtcgccg ccgggccccg cgctggccag gccgtatcga
     1081 gcgggatcgg cacggcgccg tagccgggga acgctggcac ccgcattgct ctcccatcgg
     1141 acaggggcaa cgaccccccg ccgcttctcg cggtgggtcg attgcgaccc cggaaagagg
     1201 tcgagtcaat ccatgaacca caagacgttg ttagccgcaa tgctccccct gaccctattg
     1261 ctcgccgtag gctgcgggaa ggcgccgacg accgagatcg aggcggcgga cgagacgctg
     1321 gaccgtgccg aggcggcggg agcggcggaa tacgcgccgg agtcgttcgg tgcggccgag
     1381 caagccagag cggagctcga taccgagctc aaggcgcagg aggagaagct cggcgtcttc
     1441 cgctcgtact ccaaggcgca ggagttggcc ggggcggcca aggtcgcggc cgagaaggcc
     1501 gagcaggacg cggccacggc acgcgaggac gcgcgcgagg aggcggccgc gctgatcgcc
     1561 gacgtgcggg ccagcctgac cgagacgaaa gagctgctcg agcgcgcccc ccgcggtaag
     1621 ggcagcgagg tcgatctcgc cgcgctgcaa gccgacttga ccggaatcga gaacgccgcc
     1681 gccgagctcg accgcgcgtt tgccgacggg cgatacctcg aggcgaagac cggggccgag
     1741 gccgcgatgg cgggtgccga acagatcaag agcgagatcc tgcgtgcgat ggagacggcg
     1801 aagccccgga cggctcggcg cagctgagcc ctggcttctc gcgctcgtcg ggatcgcggc
     1861 ggtcgcctgc gggctccagg cggcgagcgg acgggccacg ccgccggcct acagccgcta
     1921 cgcggccgag cgggcgctcg accgggcacg ccgggccgac gcgggggctc atgcgccgtg
     1981 gcggctgcga cgggccgacg cggcgctggc cgacgctcgg ctcgagttcc gccggcacca
     2041 gctgcgcttc tacctgtggc gcgatttcac cggcgcgcgc gagaagctgc gccgcagcga
     2101 ggccatggcg gtcgagaccg aaggcctcgc gcggcagcgg ctggcggcgg aacgggagtc
     2161 gatgcggcgc gagctcgagc acgcggcatg cgtcgtcggt ttgacggcga gcctggcgcg
     2221 gaccttccgc ttcgggaagc agacggtcga gggaatgcgg gcggcgaaga ggtatctcgc
     2281 ggaggcggag gtattcgccc gccggcagcg gttcgaccgc gcgcggctcc tttgccaacg
     2341 cgcgaacgtc gaggcgcggt cggcgaggga ccggatgacc gacgcgctcg cccgattcgt
     2401 cgacggcgac aacgtacgtc gctggaccga gcagagggcg catgcggtgc gctggtcggc
     2461 cgcgcactcg cggcccgcgc tggtcgtcaa caaggacgag gcgacgctcg cggtcttcgt
     2521 ctcggggcgg ttggtcgcga cttatcccgt cgagatcggt ctcaaccatc ttcaacggaa
     2581 gctcagggcc ggggacgagg cgacgcccga gggtatctac cacgtgacgg cgaagaaaga
     2641 tcgaggacag acgcgctacc acagggcgct cgaactgagc tatcccaacg acgacgaccg
     2701 acgccggctg gcacgactga tcgaccagcg cgtgctgccg cccggggtga cgtccggcga
     2761 cctgatcgag atccatggcg agggcgggcg ggcgcgggac tggaccctcg gctgcgtttc
     2821 gctcgacaac aacgcgatgg acgatctgtt cgaccgcgtt gcggtcggta cgccggtcac
     2881 gatcgtcggc ggcgacggtg acgacggtcg cttcagcacc ctcgccggct tcctcgcctc
     2941 gtccggagtc gagcgtgcgt gccccgacga ttgacggcgg gacgcgccga cgtcgtgccg
     3001 gcattctggt cgcggcggcc gccgccttcg ccgtctgcta cgccctcggc accggctacg
     3061 actacgcgcc ttcgggcgtc gaaccgacgc tgacggcccc ggacgcggtc gacgccgatg
     3121 cgctgcgcga gaaccggcga ctgcgcaccg cgctcgcgcg gcacacgccg cgcgaggtct
     3181 acctcgtcgt cgatcgcgcc aacaaccggt tgtacctcag acgcggcgag acgacgctgc
     3241 tcgaagcgct ctgctcgtcc ggctccggga cggtgctgca cgcacccgac gggcggagtt
     3301 ggcgcttcga cacgccgagc ggtctgttcc gcgtgcggtc gaagaccgcg aacccggtgt
     3361 ggcgcaggcc ggactgggcc ttcgtcgagg aggggcttcc gcttcccggc cggccggagg
     3421 accggttcga gtacggctcg ctgggcgagt acgcgctcca cttcggcgac ggctacctga
     3481 tccacggaac gctgtacgag cggctgctcg ggcgcagcgt gacccacggc tgcattcggc
     3541 tcggtcgcga cgacctgcgc cgagtctacg acgcggcgaa gctcggcacg ccggtctaca
     3601 tcttctgatg agccagctcg ggctggcccg tccgatcgcg gccgcggtcc tgctgctgtc
     3661 cggcgcggcc gtggcaacgg ccgtcttcgg ccagttcggc cccgagacgc tcgaacgtac
     3721 caacctccgg ttggccgagc aagtgacgct cgccgagcgc gagcggccgt acctgatgct
     3781 cgacctgtcg gctccgcggt tgcgcttgat gatgggtggc gccctgctgc gcgagttcgc
     3841 ggtttcgggc gccgaggtcg gaacgccgac cgtgctgttc gtccgccgtt cgccgccgtc
     3901 gccgttcgcc ggtcgacccc gcacgctcgc cggggtgcac ccgccgcgcc gctcgcggcc
     3961 ggacgaggtc gtcgcgccgg tcccggggag cgtcgtcgag ccgccgttgg ttccgcccga
     4021 tccggtcgag gccgagccgg cgcccgagcg gttcttcctg cgattcgatg acggcctgtc
     4081 gatcgaggta cgccaggagc gcgcggcgac cggcggctgg cgggcgcggt atacgaggct
     4141 ccgctggaag ctcgtcgaca tgctcgacgc cagccggccc ggccccgcct cgcgtttgcg
     4201 tctggtaatg cccgagtccg aggcaggttc gctctatcgc tccattccgc aagcgacacc
     4261 gttgatcgtg tcgtgaaagg tgtgatgacg atcccccgct cccggcgagc aacccccgag
     4321 ccggtcgccg tttccgttcc tgatcagtcc gcgggccggt agacgcgccg cgcgcattcg
     4381 tccagcatcc gcgtcgcgca gaaccgaggg cagatcgtgc gcagcgaggc cttgacccgc
     4441 gcgatccacc catgcgggat cccgtccgca ccccgctcgt ggaacagcgg aacgacctgc
     4501 cgctccaaca ggtcgaacag cgccgccgcg tcccgctcgt cgtccgcctc gccgccgccc
     4561 gggctgccct cgatcgcgaa gccgttcgtg ccgtcgtacg cttcggccca ccagccgtcg
     4621 agcacgctca ggttcagcgt cccgttgaac gccgccttga tgccgctcgt gccgctggcc
     4681 tcgagcggtg ggcgcggcag gttgagccag acgtcgcagc cggcgacgag caggctggcc
     4741 ttgcccaggt cgtagtcttc gaggaacgcc acgcgccccg cgacgcgtgg gtcggtcttc
     4801 agccgaaaca gctcggtcaa cagcgccttg gccacgtcgt ccttggggtg cgccttgccc
     4861 gacacgagaa gttgcagcgg tcggctcccc tcgagcaggc gcagcagccg tggcgggtcg
     4921 cgcagcagca gatgcagtcg cttgtacgtc gcgatccgcc gggcgaagcc cagggtcagc
     4981 acctcgggat cgaaggtgcg cgccgcctgc tcgacgaagt ccatcggctc gtcgcgcgtc
     5041 agccgctcgg tcaccgtctg ggagcggacc cacttcacca accgctcggc cagcgcgtta
     5101 cggaccgtcc acagctcctc gtcggggatc gcgtcgatcg ccgcccaggt cgccggatcg
     5161 cgcacgcggt ccggatcgct ccaatcggca tcgaggtgcc gatcgagcag gcgccgcatc
     5221 ggcggcgaca tccaactcgg caggtgcgcg ccgttggtga cgtgcgagat cggaacctgc
     5281 tcgacggcac tcgccgggaa cagctcgcgc cacatcgccc gcgagacctc gccgtggatc
     5341 cggctgacgc cgttgatgct ccggctcagg cggatcgccg ccggcgtcag gccgggtggc
     5401 tcgtcgcgcc gatcggggtg cacgcgcccg agggccaaca gctcctccca ctcggttccg
     5461 agctggccgg cgacgtcggg aaagacggcg cgcatctcgt cggcggagta cgtctcgttg
     5521 ccggccggga ccggcgtgtg cgtcgtgaac acgaactgcc gtcgcgcctc ggtgcacgcc
     5581 tggccgaacg acgcgccgcg ggccatcagc ccgcgcatcg cctcgatcgt cgcgagcgcg
     5641 ggatgcccct cgttgagatg aaccacgagc ggttcgatcc cgagagcgcg caggacacgc
     5701 atcccgccga cgccgagcag cgcgtactgg gccaatcgca cctggcggtt cccttcgtac
     5761 agccgcgccg agatgaatcg ctgccgcggg gtgttgcgat cgacggtcgt gtcgagcagg
     5821 aacagcggga cgcgtccgac gtcgacgcgc cacacctgga ccgcgacctc ctcggcccag
     5881 atcggcacgg tgacctggat cgggtggccg tccgcggcgg tgacgcgcac ggccggccgg
     5941 cgctcgggat cggtctcgta ccaatactcg tgctggaggc cggccgcgtc gacccgttga
     6001 tggaagtagc cctggcggta caggatgccg acaccgacca tcgggatcgc gagatcggac
     6061 gcctccttca ggatgtcgcc ggcgagacag cccagcccgc cggagtaaat cggcatcgaa
     6121 cgatggatgc cgaattcggc gcagaagaac gccaccggtc ggtcggccgg gaaaccggtc
     6181 gccgccggcc gcgcgaggtc ggccccgagg gcgtcgcgca ggcgctcgac gcgatcgacg
     6241 atacgcgggc tgagcgaggc cttctcgagc gactccggca gcgcctcttg caggaagcgc
     6301 accggattct gcccgcacag ccgccagcgg taggcgtcga tgtcgcggaa caagcgatcg
     6361 ccgtccggct gccaggacca gaagtagttg taggccaccc gagcgagcgg tgcgagacgc
     6421 gacggcacgt tcgtcgcgag cgcctgcgcg gcgcgacgca gatccttcgc gccggcctgg
     6481 ccgctggcgt tgcggaaccg ctgcatctcc atgccagccc ctcagccgtc ccgctcgccg
     6541 gcgtcctgcg cgtcgcgctc gtagaccgac acgtgctggg tgctccgcga gtcgaacggc
     6601 aggcggttcc agtcgcccca gcggtgcacg agtctcaggc ccgccagacg cgccatcagg
     6661 tcgagctccg acggccaggc gtagcgcacc tcgagcgggt aaaacttcgc gccgtgctcg
     6721 gtcagcacca cgtgacggct ggtcaccgtc tgacggaccg ggtcgtgcag cgaagcctcg
     6781 atccgtaccg cgtccggatc gtgcgcctcg agaccgatcc gctggttccg ctcgaagcgc
     6841 gcgaggtccg gcacgaacgc gtccaagacg aacctcccgc gggggtcgag gcggccggcc
     6901 acgttgcgaa aacagcgcag ctgatcctcc tgactggtca gcgcgaagag cgtgttgaag
     6961 gcgacgaaga tcaacgggta gcgtccgccg acggcgacgt cggcgaagtc gccgagcgtg
     7021 acccggagcc gatccccgcc gggtttcgac cgcagcttcg ccaccatcgc cgcggagctg
     7081 tcgatgccgt ggacctcgac accggcgcgc gacagcggta gcgcgatccg acccgtcccg
     7141 atgccgagct cgagcaccgg accggcgccg cccgccagct cgcgcaggca ggcgaccgcg
     7201 gcatcgggac cgagcgaggc gtaccagcga tcgtagacct ccgcgatcga gttcccgtag
     7261 gtcgaagggt cgtagtcgtc cacggcaacc cccttccgcc gtcatgatac gccggcgtga
     7321 cggccgcgtc aggattgcgt gacgattccg cgtcggggac gctcggccgg tccgcgaccg
     7381 gtcgcctccc cggcgccctg cgacacaatc ttcacatcgc cttaagacgt tcgtcgcgca
     7441 cgtccggtct ctttagcgcg atcgatcggg gcgagcaccc cgcacgaggc acggtgcaat
     7501 tgccacgacc gagaacgtct tcaatgggag gggacatgat ccgaaggtgg acacctcgga
     7561 ccatcggtgc gctcgtcgcg ctgacggtcc ttctcgccgc cggacccgcg ttcgcgcagg
     7621 ggctgtgcga ctcggacccg gagttgccga tcgtcccggt cgacagcgac atcacgatcg
     7681 acacgatctg gaccaacgac aacgtctacg tcctgcgcga cgtgatctac gtgcgcggcg
     7741 gggcgacgct gacgatcgag cagggcacga tcgttcgcgg cgtcccggac gcgaccacgc
     7801 cctccgtgcc gaacaacccc gggacgctgg tcatcacccg cgactcgaag atctacgcgc
     7861 tcggcacggc gcgcgaaccg atcgtcttca ccgactgcaa caacgacaac ctgcgcgact
     7921 tccgccccgg cctgttcccg tacgacgacc tggccaacgt cctgggcgag gtggcgaact
     7981 ggggcggcct gatcgtgctc ggccggacct acgtcgccaa caactcggcg ggcggcccga
     8041 acccggcgcg cgaggtgctg atcgaggggc tcgaggccaa cggcgagtgc agcaacgatc
     8101 cgtcgatcag ctgcacgtcg aacgccgact gcggcgtggg caacacctgc gacggtatcg
     8161 gccgcggctt ctacggcaac tgctcggcgg tctacgacac gctcggcgac ccctgcatct
     8221 gcgacgacga cgactccgga tcgctgcgct acctctcgat cagccacggc ggtttcaacc
     8281 tgtcgcagga caacgagatc aacggcctga cgctgggcgc cgtcggtcgc gagacggatg
     8341 tctcctacat cgaggtgttc cagagcaagg acgacggctt cgagttcttc ggcggcacgg
     8401 tcagtcccga tcatctggtc gtggccaaca ccggtgacga cggcttcgac accgacgagg
     8461 gtttccgcgg caagctgcag tacctgctgc tgatgcaggg caagccgggc accgaccagg
     8521 gcgacaaggg cgccgagatg gatggcggca acagcccgga cgccagccgg ccgatcgcga
     8581 tcccgacgat ctacaacgcg acctacgtcg gtctcggcgc gggcggcggc tcgagcaaca
     8641 cggcgctgca cttccgcgac aacaccggct tccgctacta caacagcgca ttcctcgatt
     8701 tccgcggtgc ggcggcactg gtcgaggggg cgaccgactc gaccttcccg cgcgacccgg
     8761 cgctcggaac ggcggcgaac acgagcggcg agcgggcgat cacggcgttt ccgccggcgg
     8821 gcgaggacgc ctgcttctac gcggaggtgc cgacgtcggc gacgcagctc gagctgcggg
     8881 acaacacgtt ctggtgcatc ggccggcaag agatgccggg gctcgccggg acgctgccgt
     8941 cactcggcta ctgctcgaac gtcgggccgg gtacgcgcaa ctgtctggac gacgccgact
     9001 gcggcgtggg caacacctgc gtcgacaagc cgctcgacta cggcatttcg agcggcgacg
     9061 tgaacaaggt ccacttcgat cccggctggc tcgccg
//
LOCUS       DASPGN010000416         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_661196, whole genome shotgun sequence.
ACCESSION   DASPGN010000416 DASPGN010000000
VERSION     DASPGN010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661196"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VD788_12135"
     CDS             complement(<1..648)
                     /locus_tag="VD788_12135"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXV77056.1"
                     /translation="MTHEERRFRPISGWPALFVCVALVACGPALPILLTSLAPAARAA
                     AGVIPAVAGVVMAFGFMAIAPNQSRVLLLFGAYTGSVVESGFFWVNPFYSKKKISLRI
                     RNFETGSIQHPEKKDQLGKVIEPKGRTPGRPSKVNDREGNPIEISSVVVWRVVNTAEA
                     VFEVDDYEDFVAVQSEAALRNLASRHPYDAADGTVSLRGNTTEVNDQLQHDIQERL"
     gene            764..2203
                     /locus_tag="VD788_12140"
     CDS             764..2203
                     /locus_tag="VD788_12140"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HXV77057.1"
                     /translation="MIFSSVEFLVFFGLVLAVYWGMLDRFRDPAALRPQHLFLLVASY
                     YFYMSWNALFVLLIVLSTLIDYLAGARIHASSCPRTRRALLLTSLVANLGLLAVFKYT
                     GFFLDNANAVLGLFSLRLPLALEFLLPVGISFYTFQSMSYTIDIYRGRLTPARSLTDF
                     ALFVSFFPQLVAGPIVRASQFLPQLSRPRRPTDIDVHFGLSYLLLGLVKKLLLADWLS
                     TVADPLFADPLAFNSIGAWAGVIAYTLQIYCDFSGYSDIAIGTAALLGYRLPDNFNMP
                     YLSRNVSEFWSRWHISLSSWLRDYLYVPLGGNRGGRLRTLRNLLLVMLLGGLWHGAQW
                     TFVAWGAAHGLALVVHKEFRRRFPRDPSRRPSAVGQVAGWLATMLFVCASWVLFRSPS
                     FDTARIVLTKLAFLDNRGVAPAGPLLVFCLAVVVLAHVAGRWIFADDRRAVPPRIGLP
                     LRAVGYAVVVVLLIALAPVDTRPFIYFQF"
     gene            2206..>3004
                     /locus_tag="VD788_12145"
     CDS             2206..>3004
                     /locus_tag="VD788_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77058.1"
                     /translation="MNRSRHPYLQLAAVAGIGLPVVAAGLFAIDRAFLTDPRSGELLA
                     AEQRVYRDASTSIELLDELIARDEVTWDPTNGERIATVLASLKRTDGPALLLVGSSQL
                     LVVRDDRTAAGIARRVDKVLDERLAGEVTVYNLSLGGMTLPEKRLMLDHALRRIDVDQ
                     IVVGLTLWDSASDEVRPALARLPVAPVPAPAPGFARALPSSINRAVTSRVERALGDAI
                     PLLGHRSAIQRWLASELFPRATRGGDADRGGPLDAEPVSYAYDERQRR"
BASE COUNT          396 a         1056 c          981 g          571 t
ORIGIN      
        1 caggcgctcc tggatgtcgt gctgcaactg gtcgttgacc tcggtcgtgt tgccccgcag
       61 cgacaccgtc ccgtcggcgg catcgtacgg gtggcggctc gccaggttgc gcaacgcggc
      121 ctcgctctgg acggcgacga aatcctcgta gtcgtcgacc tcgaataccg cctcggcggt
      181 gttgaccacg cgccagacga cgaccgacga gatctcgatc gggttcccct cgcggtcgtt
      241 gaccttcgac gggcggccgg gggtccgccc cttcggctcg atcaccttgc ccagctggtc
      301 tttcttttcg ggatgctgga tcgatccggt ctcgaagttg cgaattcgca gcgagatctt
      361 cttcttcgag tagaacgggt tgacccagaa gaaacccgat tcgacgacgg agcccgtgta
      421 cgccccgaac agcagcagta cgcgcgattg gttgggcgcg atcgccatga agccgaacgc
      481 catcacgaca ccggcgacgg ccgggatcac cccggcggcc gcgcgcgccg cgggagcgag
      541 ggacgtcagc aggatcggca gcgcgggccc gcaggccacg agcgcgacgc agacgaacag
      601 ggccggccaa ccggagatcg ggcggaagcg gcgttcttcg tgggtcatga tcgatttcct
      661 ccgagagata ccattatgat atcacgatgg tagcatccgt gcaaggacgt cgccctctcg
      721 accagccttg ccttcgccgg gaacggaacg gataatcgcg ccgatgatct tctcgagcgt
      781 cgaattcctg gtctttttcg gcctggtact ggcggtctac tgggggatgc tcgaccgatt
      841 ccgcgacccg gcggcgcttc gcccgcagca cctgttcctg ctcgtcgcca gctactactt
      901 ctacatgtcg tggaacgcgt tgttcgtcct gctgatcgtg ctgtcgacgc tgatcgacta
      961 cctcgccggc gcgcggatcc acgcctcctc ctgtccgcga acacgacgcg cccttctgct
     1021 aacgagcctg gtcgccaacc tgggtctgct ggccgtgttc aagtacaccg gtttctttct
     1081 cgacaacgcc aacgccgtgc tcggcctgtt ctcgctgcgc ctgccgctgg cgctcgagtt
     1141 cctgctgccc gtcgggatct cgttctacac cttccagtcg atgagctaca ccatcgacat
     1201 ctatcgcggg cggctcacgc cggcccgcag tctcaccgac ttcgccctgt tcgtttcctt
     1261 cttcccgcag ctggttgccg gcccgatcgt gcgtgcctcg cagttcctgc cccagctgtc
     1321 gcggccgcgg cggccgaccg acatcgacgt ccatttcggt ctgagctacc tgttgctcgg
     1381 gctggtcaag aagctgctgc tcgccgactg gctgagcacc gtcgccgacc cgctgttcgc
     1441 cgacccgctg gcgttcaact cgatcggcgc ctgggccggc gtcatcgcct ataccctgca
     1501 gatctactgc gacttttccg gttactccga catcgcgatc ggcacggccg cgctgctcgg
     1561 ctaccggctg ccggacaact tcaacatgcc gtacctgtca cgcaacgtct ccgagttctg
     1621 gtcgcgctgg cacatcagtc tgtcgagctg gctgcgcgac tacctgtacg tcccgctcgg
     1681 cgggaaccgc ggtggacggc tgcgcacgct gcgcaatctg ctgctggtga tgctgctcgg
     1741 cgggttgtgg cacggcgccc agtggacctt cgtcgcctgg ggcgcggccc acggcctcgc
     1801 gctcgtcgtc cacaaggagt tccgccggcg tttcccgcgc gacccgtcgc gccgcccctc
     1861 cgcagtgggc caggtcgcgg ggtggctggc cacgatgctg ttcgtctgcg ccagctgggt
     1921 gttgtttcgc tctccctcgt tcgacacggc acgcatcgtg ctgacgaagc tggcctttct
     1981 cgacaaccgc ggggtggcgc cggcgggtcc gctgctcgtg ttttgcctgg cggtcgtggt
     2041 gctggcgcac gtcgcgggtc ggtggatctt cgccgacgat cgccgcgccg tgccgccgcg
     2101 gatcggcctc ccgttgcgcg ccgtcggcta tgcggtcgtc gtcgtgctgc tgatcgcgct
     2161 cgctcccgtc gacacgcgtc ccttcatcta cttccagttc tgaccttgaa ccgctcacgc
     2221 catccgtacc tgcagctggc cgcggtggcc ggcatcggcc tgccggtcgt cgccgccggg
     2281 ctgttcgcca tcgatcgtgc gttcctgacc gatccgcggt cgggcgagct gctcgcggca
     2341 gagcaacggg tctaccgcga cgcgtcgacg tcgatcgagc tgctcgacga gctgatcgca
     2401 cgtgacgagg tgacctggga cccgaccaac ggcgagcgca tcgcgacggt gctggccagc
     2461 ctgaaacgga ccgacgggcc cgcgctgctg ctcgtcggct cctcgcaact gctggtggtg
     2521 cgggacgatc gcacggcggc gggcatcgca cggcgggtgg acaaggtgct cgacgagcga
     2581 ctggccggcg aggttaccgt gtacaacctg tcgctcggcg gaatgacgct gccggagaag
     2641 cgcctgatgc tcgaccacgc gctgcgtcgg atcgacgtgg accagatcgt cgtcggcttg
     2701 acgttgtggg attcggcgtc ggacgaggtg cggccggcgc tcgcgcggct gccggtcgcc
     2761 cccgtcccgg cccccgcacc cggcttcgct cgggcgttgc cgtcgtcgat caaccgcgcg
     2821 gtcaccagcc gcgtcgagcg ggcgctcggc gatgcaatcc cgctcctcgg ccaccggtcc
     2881 gcgatccagc gctggctcgc gagcgagttg tttccgcgag cgacgcgcgg cggcgacgcg
     2941 gaccgcggcg ggccgctcga cgcggagccg gtctcctacg cctatgacga gcggcagcgg
     3001 cgcg
//
LOCUS       DASPGN010000417         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_427427, whole genome shotgun sequence.
ACCESSION   DASPGN010000417 DASPGN010000000
VERSION     DASPGN010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427427"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..834
                     /locus_tag="VD788_12150"
     CDS             166..834
                     /locus_tag="VD788_12150"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXV77059.1"
                     /translation="MVQNSNTGSTDRLQRRAETRRTEILAAAARVFRRRGYADAGMRE
                     IAAEADLSPGNLYHYFRGKEEILCFCQQRALERLLGALERASRPGLPVAARLGQLLHA
                     HVICLLDEFEGAAAHLEPEAVPAETRAAIVEQRDRYERGVRQLITAGVASGEFTPCDE
                     RVVTRAMLGALNWSARWFRPDGPDSPSRIADELSRYLVRGLASGRRGPPAHTGAAGPG
                     VGHG"
     gene            827..1354
                     /locus_tag="VD788_12155"
     CDS             827..1354
                     /locus_tag="VD788_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXV77060.1"
                     /translation="MADPIVDDPTENVRLSLEVNGERTEVSFAPYKTLLEVLREELRL
                     TGTKHGCELGECGACAVLVDGRPLLSCLLLALDCAGRSVTTVEGLGDGAELDPLQAAF
                     ADLGGSQCGYCTPGILLTARALLDRTPRPNRDQIREALSGNLCRCTGYQQIFESVEDA
                     IVRVARDRTIPGVDR"
     gene            1351..>2237
                     /locus_tag="VD788_12160"
     CDS             1351..>2237
                     /locus_tag="VD788_12160"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXV77061.1"
                     /translation="MNDDGRFDRVGRPRRRIDGRAKVTGRTLFADDLDFPRMLHCRLL
                     RSTHPHARILAIRTERARAIDGVRLVLTGRDLRGEFGILPVSQDEEPLCREKVRYVGD
                     PVAAAIARDEVTAQRAVEAIEVDYEPLTTIADPAQALAVVEPRIHDYGEEGNVHKRVS
                     LCFGDVDRALEQADHVFDDLFFYEGNTHLPLEQHAAVAAVDADGKLTLWSSTQTPHYV
                     HRALAKVLDMPPSRIRVIACHNGGGFGGKSDIFNHEIVVSKAALVLGRPVKIALNREE
                     VFYCHRGRHPVLMRFRTGVT"
BASE COUNT          338 a          760 c          802 g          337 t
ORIGIN      
        1 atcccgaacc ggccgccgac gcccgccccc cccgcgcgcg ccgccggcgc cgctcccgcg
       61 ggcgccgggg cggcccggac gaccgcggtc gtccccggca ggacggcgag gactgaggcg
      121 cgaaagccgc ttgacgttcc gcccggtcgt gggacaatgt gaaccgtggt tcaaaactcg
      181 aacaccggtt cgaccgaccg tttgcaacga cgggccgaga cacgccggac cgagatcctc
      241 gcggccgcgg cgcgggtgtt ccgacgacga gggtacgccg acgcggggat gcgggagatc
      301 gcggccgagg ccgatctgtc accgggcaat ctctaccact acttccgcgg gaaggaggag
      361 atcctgtgct tctgccagca gcgcgccctc gagcgactgc tcggggcgct cgagcgagcg
      421 agccggccgg gacttccggt cgccgcgcgc ctgggccagc tgctgcatgc tcacgtgatc
      481 tgtttgctcg acgagttcga gggagcggcg gcgcacctcg agcccgaagc ggttccggca
      541 gagacgcgcg cggcgatcgt cgagcagcgc gaccgttacg agcgcggcgt gcgtcagctg
      601 atcacggcgg gagtggcgag cggtgagttc acgccgtgcg acgagcgcgt ggtgacccgt
      661 gccatgctcg gcgcgctcaa ttggtcggca cgctggttcc ggccggacgg gccggattcg
      721 ccgtcgcgga ttgccgacga gctgtcgcgc tatctcgtcc gcgggctcgc gagcgggagg
      781 cgtgggccgc cggcgcacac cggggccgcg ggtccggggg tcggtcatgg ctgatccgat
      841 cgtcgacgat ccgaccgaga acgtccggct ttcgctcgag gtgaacggcg aacggacgga
      901 ggtgtcgttc gcgccttaca agacgctgct cgaggtgttg cgggaagagc tgcggctgac
      961 cggaaccaaa cacgggtgcg agctcggcga gtgcggggcg tgcgccgtgt tggtcgatgg
     1021 acgcccgctg ctgtcgtgct tgctgttggc cctcgattgc gcgggacgat cggtgacgac
     1081 ggtcgagggt ctcggcgacg gtgccgagct cgacccgctg caggccgcct tcgccgacct
     1141 cggcgggtcc cagtgtggct actgcacgcc cgggatcctg ctcaccgcgc gcgcgctgct
     1201 cgatcgcacc ccgcggccga accgcgacca gattcgcgag gcgttgtccg gcaacctctg
     1261 ccgctgtacg ggctaccagc agatcttcga gtcggtcgaa gacgcgatcg tccgcgtcgc
     1321 gcgcgaccgg acgatcccgg gggtggaccg gtgaacgacg acggacgctt cgatcgggtc
     1381 ggtcgccctc gccggcggat cgacggccga gccaaggtga ccgggcgcac gctgttcgcg
     1441 gacgatctgg attttccacg gatgctccac tgccgactgc tccgctcgac ccatccccac
     1501 gcccgcatcc tcgccatccg gaccgagcgc gctcgagcga tcgacggtgt ccgactggtg
     1561 ttgaccggcc gggacctgcg cggcgagttc ggcatcctcc ccgtcagtca ggatgaagag
     1621 ccgttgtgcc gcgaaaaggt acgttacgtc ggcgatccgg tggcggccgc gatcgcgcgc
     1681 gacgaagtga ccgcccagcg ggcggtcgag gcgatcgagg tcgactacga gccgctgacg
     1741 acgatcgccg acccggcgca ggctctcgcg gtggtcgagc cgcggatcca cgactacggc
     1801 gaggagggca acgtccacaa acgtgtgtcg ctctgtttcg gcgacgtcga tcgggcactc
     1861 gagcaggcgg atcacgtctt cgacgacctg ttcttctacg agggcaacac ccacctgccg
     1921 ctcgaacagc atgctgcggt cgccgccgtc gacgccgacg gcaagttgac tctgtggtcg
     1981 agcacgcaga cgccgcacta cgtgcaccgc gcgttggcca aggtgctcga catgccgccg
     2041 agccggatcc gcgtgatcgc ctgccacaac ggcggcggct tcggcggaaa aagcgacatc
     2101 ttcaaccacg agatcgtggt gtcgaaggca gcactggtcc tcggccgacc ggtgaagatc
     2161 gcgctcaacc gcgaggaggt cttctactgc caccgcggac ggcatccggt actgatgcgc
     2221 ttccgcaccg gggtgac
//
LOCUS       DASPGN010000418         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_311433, whole genome shotgun sequence.
ACCESSION   DASPGN010000418 DASPGN010000000
VERSION     DASPGN010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311433"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..918)
                     /gene="nth"
                     /locus_tag="VD788_12165"
     CDS             complement(238..918)
                     /gene="nth"
                     /locus_tag="VD788_12165"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXV77062.1"
                     /translation="MARGAPGGRQPDKPDPRLMHRVMRTLAARYEIDRFLQLNGPDPY
                     RILVGCVISLRTKDEVSYAATARLFARARTPAEMIRLRTATVAELIYPAGFYNRKAKQ
                     IREISRLLTRRHAGRVPDEIDELLALPGVGRKTANLVVTLGFGNPGICVDTHVHRITN
                     RLGWVATDHPDRTEFALRESLPRRLWIPINETLVRHGQQVCRPVSPLCSRCPVASDCA
                     RRGVERSR"
     gene            complement(925..1566)
                     /locus_tag="VD788_12170"
     CDS             complement(925..1566)
                     /locus_tag="VD788_12170"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXV77063.1"
                     /translation="MSDTGDHPFTELIRGRTTVERFDARRGLDETEVRALVRDAVQAP
                     SSFNIQHWRFVAVRRPDDKRRLMEAAYGQQQVADAAVTFIVLGDTRGADKLPLIVERA
                     VGQGALADGKAAAWVRLAERIYADPTVARDEAIRSASLAAMVLMLAAEARGLGSGALS
                     GFDAGRVRREFAIDQRYVPVMLLCVGHPASVSGERQPRLSVDEVLSFDRGRVF"
     gene            complement(1579..2139)
                     /locus_tag="VD788_12175"
     CDS             complement(1579..2139)
                     /locus_tag="VD788_12175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77064.1"
                     /translation="MQTHAMPTIALRFSLGFGMVTLLAALVFRSNVFLPASPMFRSLT
                     AGAMLAGVLALMRSDRGPQAGALATALALLRLGFAHSQGWTAAVAGVFEVGGLFLVAL
                     IFDLLARRGILFGKFLVLGPLLGGVYLATTQLSVMFQVTGVELMPTLMRHVFIGLLIG
                     DSVGFGIEVADLLVLARSVGQQRSTG"
     gene            complement(2143..>2830)
                     /locus_tag="VD788_12180"
     CDS             complement(2143..>2830)
                     /locus_tag="VD788_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77065.1"
                     /translation="VGGKPYVTGINIQQALLTLIEGEKVDWEVTASAGRELRRETIHL
                     NTGKMLFLCAGAYEALYDQVFRRVTSPTSRVKLPTETVLIDGQVEIREFFSLRHHFKL
                     EDLFDYGMQPQFLSRFDNTLILEELDADMLKRILLEPRDSVFNASKNFFRKYDIDLQI
                     TDGAALRIAYAAAEAKRIGARALKEVYGRIIKPFEYDPFARDELQQTASGRRLVIDEQ
                     LVTRTLNPAD"
BASE COUNT          482 a          973 c          947 g          428 t
ORIGIN      
        1 cacgtcgcgg tggtccatgc tcagcaccag cccgcggtag gcgtatcgcg agcagtcctc
       61 gccccgggcc ggtgtcgcgc cgtggcacag cgccagcagg acgaagcagg tggccgggcg
      121 gatcggcatt cgagctcccc gagaccggtc gaagggcggc acggccttcg agctacggta
      181 cgcacgggct gcccgctttt cacccgcgat ctcgccggcg ggccgttcgg ccgcggctca
      241 gcggctccgc tcgacgccgc gccgcgcgca gtcggacgca acgggacagc gtgagcacag
      301 cggcgagacg gggcggcaca cctgttggcc atgtcgcacc agcgtctcgt tgatcggaat
      361 ccacagacgg cgcggcaacg actcgcgcag cgcgaactcg gtgcgatcgg ggtgatcggt
      421 cgcgacccag ccgaggcggt tggtgatgcg atgcacgtgg gtgtcgacgc agatcccggg
      481 gttgccgaag ccgagcgtga cgaccagatt ggccgtcttg cggccgacgc ccggcaacgc
      541 gagcagttcg tcgatctcgt cgggaactcg gcctgcgtgt cggcgggtca acaaccggct
      601 gatctcgcgg atctgcttcg ccttgcggtt gtagaagccg gccggataga tcagctcggc
      661 cacggtcgcc gtccgcagcc ggatcatctc ggccggcgtc cgcgcccgtg cgaacagccg
      721 ggccgtcgcc gcgtagctca cctcgtcctt ggtgcgcagc gaaatgacgc agccgaccag
      781 gatgcgatac gggtctgggc cgttgagctg gaggaaccga tcgatctcgt atcgtgcggc
      841 aagcgtccgc atcacgcggt gcatcagccg gggatcgggt ttgtccggct gcctgccgcc
      901 gggcgcgccg cgcgccatgc cgactcagaa tacgcgaccg cggtcgaacg acagcacttc
      961 gtcgaccgac aggcgtggtt gccgctcccc ggacacgctc gcggggtgcc cgacgcacaa
     1021 cagcatcacc ggaacgtagc gctggtcgat ggcgaactcc cgccgaaccc gtcccgcgtc
     1081 gaacccgctg agcgcccctg agccgagccc gcgcgcctcc gcggccagca tcagcaccat
     1141 ggcggccagc gatgcggacc gtatcgcttc gtcgcgagcc accgtcgggt cggcgtagat
     1201 ccgttccgcc agccgaaccc aggccgcggc cttgccgtcc gccagtgcac cctgcccgac
     1261 cgcgcgctcg acgatcagcg gcagcttgtc tgcaccgcgc gtgtcgccca acacgatgaa
     1321 ggtcacggcc gcgtcggcca cctgttgctg accgtaggcc gcttccatca accgccgctt
     1381 gtcgtccggg cggcggacgg cgacgaagcg ccagtgctgg atgttgaacg acgacggcgc
     1441 ctgcaccgcg tcgcgtacca gcgcacgaac ctccgtctcg tcgagtccgc gccgcgcgtc
     1501 gaaccgttcg acggtggtgc ggccgcggat gagctcggtg aatgggtggt cgccggtgtc
     1561 gctcatcgat cgtgactcct acccggtcga gcgctgctgc ccgacggacc gcgccagcac
     1621 gagcagatcg gcgacctcga tgccgaaacc gacggaatcg ccgatcagca acccgatgaa
     1681 gacgtggcgc atcaaggtcg gcatcaactc gacgccggtc acctggaaca tcaccgacaa
     1741 ctgcgtcgtc gcgagataga cgccaccgag cagcggtccg aggacgagaa atttgccgaa
     1801 cagaataccg cggcgcgcga gcaggtcgaa gatcagcgcg accaggaaca gcccgccgac
     1861 ctcgaacacg ccggcgacgg cggccgtcca tccctgggag tgggcgaagc ccagccgcag
     1921 cagtgcgagg gcggtggcca gcgcgccggc ctggggcccg cggtccgatc gcatcagcgc
     1981 gaggactccc gccagcatcg caccggccgt cagcgagcgg aacatcggcg aggccggcag
     2041 gaagacgttc gaacgaaaga cgagcgcggc cagcagcgtc accatgccga accccagcga
     2101 gaagcgcagt gcgatcgtcg gcatggcgtg cgtttgcacg gttcagtcgg ccggattcaa
     2161 cgtacgcgtc accagttgct cgtcgatcac caaccgcctg ccgctcgccg tctgctggag
     2221 ctcgtcacgc gcgaacggat cgtactcgaa cggcttgatg attcggccgt agacttcctt
     2281 gagcgcccgg gcgccgatgc gcttggcttc ggccgcggcg taggcgatgc gcagcgccgc
     2341 gccgtcggtg atctgcaggt cgatatcgta cttgcggaag aagttcttcg acgcgttgaa
     2401 aacgctgtca cgcggttcga gcagtatgcg cttgagcatg tcggcgtcga gctcctcgag
     2461 gatcagcgtg ttgtcgaaac gcgagaggaa ctgaggctgc atcccgtagt cgaacagatc
     2521 ctccagcttg aagtgatgtc gcaacgagaa gaactcgcgt atctcgactt gaccgtcgat
     2581 cagcacggtc tcggtcggta gcttgacccg actggtcggc gaggtcaccc ggcggaacac
     2641 ctgatcgtac aacgcctcgt aggcaccggc gcagaggaac agcatcttgc ccgtgttcag
     2701 gtggatcgtc tcgcgccgca gctcccgacc ggccgaagcg gtcacctccc agtcgacctt
     2761 ctcgccctcg atcagcgtaa gcaacgcctg ctggatgttg atcccggtga cgtacggctt
     2821 gcccccgacc
//
LOCUS       DASPGN010000419         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_311541, whole genome shotgun sequence.
ACCESSION   DASPGN010000419 DASPGN010000000
VERSION     DASPGN010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311541"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VD788_12185"
     CDS             complement(<1..376)
                     /locus_tag="VD788_12185"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28"
                     /protein_id="HXV77066.1"
                     /translation="MTDRQADRLLERLSTAAGPPGAEDEVRAIVRRALASVGRLSHDR
                     LGSVLCERRGTADTPRIVLDSHLDEVGFMVQSVRPDGGLAFVPLGGWWGHVLLGQRVE
                     ILTADGRVPGVIGSKPPHFLSPD"
     gene            455..2083
                     /locus_tag="VD788_12190"
     CDS             455..2083
                     /locus_tag="VD788_12190"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: protein motif:HMM:NF005482.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HXV77067.1"
                     /translation="MQRAVSALERARLAYRPRLPPILRSGLDRLAVEEAGATTATRDA
                     DLIRARFPRTFGRPIVRLGSGRSPMPSASMTVGVVLSGGQAPGGHNVITGLFDALRSM
                     HADSRLLGFLGGPRGVVEGNQRELTEPLLAPFRNTGGFDLIGGGRDKIETTEQFAACR
                     ATCLGLGLDGLVIVGGDDSNTNAAMLAEYFVEHDVRTAVVGVPKTIDGDMRGNGIETS
                     FGFDTATKTYSELIGNICRDAASAGKYWHFVKLMGRNASHVALECALSTRVNVALIGE
                     EVERDATTLEQIVERIAQVIRARAATGRGYGVCIVPEGLIEFIPEMRALIAELNRLLA
                     DPSAAGPVDRRETIVAASLGNGGRRAFASLPEAIRHQLLFERDAHGNVQVSLIETERL
                     LVEKVRERLGGLRAQQHFFGYEGRSAAPSNFDADYTYALGHVAAALIALGRTGYVCAV
                     QDLHLPPDRWRAAGIPLTSLMQIEQRRGRATPVIGKALVDLGGAPFGELAAERERWSI
                     EDAYLYPGAIQYFGPDEICNRPTRTLELEAGARR"
BASE COUNT          322 a          770 c          765 g          313 t
ORIGIN      
        1 cgtcggggga gaggaaatgg ggcggcttgc tgccgatgac gccgggcacg cggccgtccg
       61 cggtcaagat ctcgacacgc tggccgagca gcacgtgccc ccaccagccg cccaggggga
      121 cgaacgccag cccgccgtcg ggtctcacgc tctgcaccat gaaaccgacc tcgtcgagat
      181 gactgtccag cacgatccgc ggcgtatcgg ccgtgccgcg gcgctcgcac agcacgctgc
      241 ccagccggtc gtgggacagc cgaccgaccg aagccaacgc gcgacggacg atcgcgcgta
      301 cctcgtcctc ggcgcccggt ggtccggcgg cggtgctgag ccgctcgagc agtcgatcgg
      361 cctgtcggtc ggtcatcgcc ggcgaggata ccgcatcccg cacgtggcct ataatcggtt
      421 ctcccgtgac ccgcacggcg gaggttccga cccgatgcaa cgtgccgtat cggcgctcga
      481 acgggcgcgt ctcgcatacc gtccccgact gccgccgatt ctccgctccg gcctcgaccg
      541 cctggcggtc gaggaggcgg gtgccacgac cgccacgcgc gacgccgatc tgatccgcgc
      601 gcgcttcccg cgcaccttcg gccgtccgat cgtccggctc gggagcggtc gttcgccgat
      661 gccgagcgca tcgatgaccg tcggcgtcgt gctcagcggc ggtcaggcgc ccggcgggca
      721 caacgtcatc accggtctgt tcgacgcgct ccgatcgatg cacgccgact cgcgcctgct
      781 cgggtttctc ggcggtccgc gcggtgtcgt cgagggcaac cagcgcgagc tgaccgaacc
      841 actgctcgct ccgttccgca acacgggcgg cttcgatttg atcggcggcg ggcgcgacaa
      901 gatcgagacg accgagcagt tcgccgcgtg ccgcgccacc tgcctcgggc tcgggctcga
      961 cgggctggtc atcgtcggcg gcgacgactc gaacaccaac gccgcgatgc tcgccgagta
     1021 tttcgtcgag cacgacgtgc gcacggcggt cgtcggcgtc ccgaagacga tcgacgggga
     1081 catgcgcggc aacgggatcg agacctcgtt cgggttcgac accgcgacga agacctattc
     1141 ggagctgatc ggcaacatct gccgcgacgc cgccagcgcg ggaaagtact ggcacttcgt
     1201 caagctgatg gggcgaaacg ccagccacgt ggcgctcgag tgcgccttgt cgacgcgcgt
     1261 caacgtggcg ctgatcggcg aagaagtcga gcgtgacgcg acgacgctcg agcagatcgt
     1321 cgagcgaatc gcgcaggtca ttcgcgcgcg tgccgcgacg ggccgggggt acggcgtctg
     1381 catcgttccc gagggtttga tcgagttcat cccggagatg cgggcgctga tcgcggagct
     1441 gaaccggctg ctggccgacc cgtcggccgc cgggccggtc gaccggcgcg aaacgatcgt
     1501 cgccgcgtcg ctcggcaacg gcggtcgccg ggcgttcgcc agcctgcccg aggcgatccg
     1561 ccaccagctg ctgttcgaac gcgacgcgca cggcaacgtg caggtcagcc tgatcgagac
     1621 cgagcgcttg ctcgtcgaaa aagtgaggga gcgcctcggc gggctgcgtg cgcaacagca
     1681 tttcttcggc tacgaggggc gatcggcggc gccgagcaac ttcgatgccg actacaccta
     1741 tgcgctcggc cacgtggccg ccgcgctgat cgcgctcggc cgcacgggct acgtctgcgc
     1801 cgtgcaggat ctgcacctcc cgcccgaccg ctggcgcgcg gcgggcatcc cgctgacctc
     1861 gctgatgcag atcgagcagc gcaggggccg cgcgaccccg gtcatcggca aggcgctggt
     1921 cgacctcggc ggcgccccgt tcggcgaact cgccgccgaa cgcgagcgct ggtcgatcga
     1981 ggacgcctac ctctatccgg gtgcgatcca gtatttcggc ccggacgaga tctgcaaccg
     2041 cccgacgcgg acgctcgagc tcgaggccgg cgctcgccga tgagaggcgt cgcgacgccg
     2101 ggggtccgag ggtgacggcg cggctcgtct actcgagcga tcgcgggcgg ccggcggcct
     2161 gcgagcggtg
//
LOCUS       DASPGN010000420         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1011441, whole genome shotgun sequence.
ACCESSION   DASPGN010000420 DASPGN010000000
VERSION     DASPGN010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011441"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VD788_12195"
     CDS             <1..1352
                     /locus_tag="VD788_12195"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXV77068.1"
                     /translation="QKFQALVEEQREIMVKDEFDEIYTTAGASSMNAGTTEDLTLYFI
                     TVPANKLELWFWMESDRLLNPIMREFYSERDVVHEERRLRTESTPTGKFDEQFDAMFW
                     QSHPYSWPVVGWPSDLRTISKEQADEYYATYYAPNNITVAMAGNFDVARVKQLAQQYF
                     GRLARGEREPPDVVTLEMEQLAEKRMEAECDCQPQIEVRYHTAPFMHGDSYALDVLAG
                     LMNGRTGRLFKSMVLGQEIASSAFATQQSRKFAGAFAFGAETKGDATPEQLEAAWFAE
                     VERLRSEPIPADELQKVKNQIAADAFRRLQNPFFLMLQLLFYDGLGDWRYMNEWSDLT
                     LAVTDADVKRVAETYFTPANRAVAVYYRKEGAEAEQWPPALAQLPADQQQMVKAQLRQ
                     VQQVDDPERLQGILGQIQQQKAAMPPQMLPAVEIIEQFIQQRIEELTAAGAAPEGGA"
     gene            1352..>2630
                     /locus_tag="VD788_12200"
     CDS             1352..>2630
                     /locus_tag="VD788_12200"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXV77069.1"
                     /translation="MSRSWRLAAALVVIALIGAPGPATLAAKKAKKSVPAHPDELRYG
                     PLDFEVPDAADYRHELSNGIPVYIAEDHALPLVTVSITLRAGGFLDTEDKPGVATSTG
                     TMIRLGGAGEMSAEEFDEKVDFLAAEISTSTGDLSSSASFNCITDVVDETAELFFTML
                     KRPRFQQDRIDVERENFLEEMKQRNDSPQSISSREWGWLMRGRDFFAARAITRDELFA
                     ITRDDLIAFHERYWRPPNMLIAVSGDVDTEQFLAKLEQWFAGWETDGPQVPWPPPRPE
                     HTPAPGVYYVDKEIPQGRVLLGHLGYERNGWDDPEAFSLSIMNDILGGGGFTSRMVKR
                     IRSDEGLAYSAGSSYGVGQYWPGVFQAFYQSKSDTVAFAAEIALEEIDRIRQGEVSED
                     ELTTAKNSFIDVFPRRFESAGQIVGTFVDDEYDG"
BASE COUNT          477 a          897 c          881 g          375 t
ORIGIN      
        1 agcagaagtt ccaggcgctc gtcgaagagc aacgcgagat catggtcaag gacgagttcg
       61 acgagatcta caccaccgcc ggcgcgtcga gcatgaacgc cggcacgacc gaggacttga
      121 cgctctactt catcaccgtg ccggccaaca agctcgagct gtggttctgg atggaatcgg
      181 accgactgct caacccgatc atgcgcgagt tctacagcga gcgcgacgtg gtgcacgagg
      241 agcgccggct gcgcacggaa tcgacgccga ccggcaagtt cgacgagcag ttcgacgcga
      301 tgttctggca gtcgcacccc tacagctggc cggtcgtcgg ttggccgtcc gacctgcgca
      361 cgatcagcaa ggagcaggcc gacgagtact acgccaccta ctacgcgccg aacaacatca
      421 cggtggcgat ggccggcaac ttcgacgtcg cccgggtcaa gcagctcgcg cagcagtact
      481 tcggccggct ggcccgcggc gagcgcgagc cgcccgacgt cgtcacgctc gagatggagc
      541 agctcgccga gaagcggatg gaggcggagt gcgactgcca gccgcagatc gaggtgcgct
      601 accacaccgc gccgttcatg cacggcgact cgtacgcgct cgacgtgctg gccggtctga
      661 tgaacggccg caccgggcgg ctgttcaagt cgatggtgct cggccaggag atcgcgtcct
      721 cggccttcgc cacccagcaa tcgcgcaagt tcgccggcgc gttcgccttc ggcgccgaga
      781 ccaagggcga cgccacgccc gagcagctcg aggcggcatg gttcgccgag gtcgagcggt
      841 tgcgcagcga gccgatcccg gccgacgagc tgcagaaggt caaaaaccaa atcgcggccg
      901 acgccttccg ccggctgcag aacccgttct tcctgatgct gcagctcctg ttctacgacg
      961 ggctgggcga ctggcgttac atgaacgagt ggtccgacct gaccctcgcc gtgaccgacg
     1021 ccgacgtcaa gcgcgtggcc gagacctact tcaccccggc caaccgcgcc gtcgcggtct
     1081 actaccggaa ggagggcgcc gaggccgagc agtggccgcc ggcgctggcc cagctccccg
     1141 ccgaccagca gcagatggtc aaggcgcagc tgcgacaggt ccagcaggtc gacgatccgg
     1201 aacggctgca ggggatcctc ggccagatcc agcagcagaa ggcggcgatg ccgccacaga
     1261 tgctgcccgc ggtcgagatc atcgaacagt tcatccagca gcggatcgag gagctgacgg
     1321 cggcgggcgc ggcccccgag ggaggtgcgt gatgtcgagg tcgtggaggt tggccgccgc
     1381 gctggtcgtg atcgccctga tcggggcgcc cggcccggcg acgctcgcgg ccaagaaggc
     1441 gaagaagtcg gttccggcgc accccgacga gctgcgctac ggcccgctcg acttcgaggt
     1501 gcccgacgcc gccgactacc gtcacgagct gagcaacggc atcccggtct acatcgccga
     1561 ggatcacgcg ctgccgctcg tcaccgtgtc gatcacgctg cgggccggcg gcttcctcga
     1621 caccgaggac aaacccggag tggcgacgag caccggcacg atgatccggc tgggcggagc
     1681 gggcgagatg agcgccgagg agttcgacga gaaggtcgac ttcctcgcgg ccgagatcag
     1741 cacctcgacc ggcgatctca gctcgagcgc ctcgttcaac tgcatcaccg acgtcgtcga
     1801 cgagaccgcc gagctgttct tcaccatgct gaagcggccg cgcttccagc aggaccgcat
     1861 cgacgtcgag cgcgagaact tcctcgagga gatgaagcag cgcaacgaca gcccgcagtc
     1921 gatctcctcg cgcgagtggg gctggctgat gcgcgggcgc gatttcttcg ccgcgcgggc
     1981 gatcacccgg gacgagctgt tcgccatcac ccgcgacgac ctgatcgcgt tccacgagcg
     2041 gtactggcgc ccgccgaaca tgctgatcgc ggtctccggc gacgtcgaca ccgagcagtt
     2101 cctggccaag ctcgagcagt ggttcgccgg ctgggagacg gacgggccgc aggtgccgtg
     2161 gccgccgcca cgccccgagc acacaccggc acccggcgtc tactacgtcg acaaggagat
     2221 cccccagggt cgcgtgctgc tcggtcatct cggctacgag cgcaacggct gggacgaccc
     2281 cgaggcgttc tcgctgagca tcatgaacga catcctcggc ggtggcggct tcacctcgcg
     2341 gatggtcaaa cgcatccgct cggacgaggg gctggcctac agcgccggtt cgagctacgg
     2401 cgtcggccaa tactggcccg gcgtgttcca ggccttctac cagtcgaaga gcgacacggt
     2461 ggccttcgcc gccgagatcg cgctcgaaga gatcgatcgc atacgccagg gcgaggtcag
     2521 cgaggacgag ctgacgacgg ccaagaactc gttcatcgac gtcttcccgc gccgcttcga
     2581 atcggccggg cagatcgtcg gcaccttcgt cgacgacgag tacgacggca
//
LOCUS       DASPGN010000421         6381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_545083, whole genome shotgun sequence.
ACCESSION   DASPGN010000421 DASPGN010000000
VERSION     DASPGN010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545083"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VD788_12205"
     CDS             <1..779
                     /locus_tag="VD788_12205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HXV77070.1"
                     /translation="EPTTENRTMIDLSGKTALVTGGSRGIGKACCELLARAGARVAVN
                     YRVERPWAELLVQRIEERGGQAFCLAADVSRPDEANMMVDETVDRFGRLDVLVNNAGV
                     WRGDPVEEISDNEWHETVAINLTGTFHMIRSAVPYMKSSGGGRIINISSTAGQRGEAF
                     HCHYAATKGAIIALTKSLASELAADGILTNCVAPGWVETDMTEQVLERDRATIVSAIP
                     LRRVARPEEIAAVVVFLASDLSSFINGEVVNVNGGSVLCG"
     gene            complement(792..1424)
                     /locus_tag="VD788_12210"
     CDS             complement(792..1424)
                     /locus_tag="VD788_12210"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV77071.1"
                     /translation="MTRGFDDWSGWIRGDRDGGADDRAPYEIVNEILAAVRDRQTKTV
                     ACFGRPTPARLRFLAQCFARVVAIDRRAGEHDPAGDEPNVEYRRRAPFDLEATDRGFD
                     VALVIASLDQDDLAEIDRRLSAIHASLAEGGLMIASVPAAPRGPAAREMRLAGRADGP
                     PRAFHEVELQYRLRRAGFRGVRVRRFGDEDASIDQPRERLLSVAVRRANN"
     gene            complement(1535..2794)
                     /gene="rho"
                     /locus_tag="VD788_12215"
     CDS             complement(1535..2794)
                     /gene="rho"
                     /locus_tag="VD788_12215"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXV77072.1"
                     /translation="MSAQKEGRRRSRNRSRGGRRRGGRGTGGHHHQRQPGGNWVATIS
                     RAPQVEPSVEVVGVLQIVEAGHGFLRSTERDLNADPSDPVVPVEIIREQGLATGLELS
                     GLAAPAEQHGRAPWLLRIGSINGEPPEQFGTQTPFKDLVAEDPTERIRLETVGEELST
                     RVVDLISPIGKGQRCLVVAPPKAGKTVLLQKIAHAISVNHPEIHLIVLLVDERPEEVT
                     DMRRNVRGEVIASSSDEMARTHVQIAEIVLERAKRLVEYGRDVVVLLDSITRLSRAYN
                     AEQRNSGRVLSGGIDARTMEKPRRFFGAARKAHGGGSLTVIGTALVDTGSRMDEVIFQ
                     EFKGTGNTEVVMDRRLFERRIFPSIDISSSGTRKEEKLFSPTEYKMVTLLRRALAPLK
                     PAEAMQVLTERLAKHRSNAEFLERMQS"
     gene            complement(2810..3298)
                     /locus_tag="VD788_12220"
     CDS             complement(2810..3298)
                     /locus_tag="VD788_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77073.1"
                     /translation="MTRVGPSSFVLDCPGRPALRIEPYAGRWRAAAGGTASGWVLERT
                     GDENGPFRLFDGDGGTELGCTSSLGGAAQRRELRYLLMGDGRLFRLSPRGPAEAGYDL
                     SGWETPGAYLTARALEGGWQILPTPASGGIDDLLAVVVLLSAEILDAEERLVCTDGSE
                     TR"
     gene            complement(3322..4026)
                     /locus_tag="VD788_12225"
     CDS             complement(3322..4026)
                     /locus_tag="VD788_12225"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXV77074.1"
                     /translation="MLLLLVACQENRNEKISAIYDLKAQPTTENVERIRELLAAPDRD
                     VRATALNALVTLGVDDARELALRHLADEDGFVRATAAKLVGDVGGSEDAAVLVARLTQ
                     DSDPVVRLRAAEALTRVGGDEAVDGLGRGLSDPIDDVRLACARGVREMNPGAAIPQLA
                     RLLLEDPNYEIRVQAARALGGTGDPDVRPLLEAALEDKNEFVRAAAANALRTHEIVRE
                     RREGEAQVDPATPAPE"
     gene            4143..4652
                     /locus_tag="VD788_12230"
     CDS             4143..4652
                     /locus_tag="VD788_12230"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77075.1"
                     /translation="MPILSAETQFKKGLAALVDHNYKDASVFFRRAIEADSARSGKKP
                     DLRYLSYYGLSLSKSGGSTTEAIRLCRAAVAKHRNHPVLLLNLGRVYLEAGKHSHALD
                     AFDRALRLAPDNQTLVRELKKLDRRAAPVLGMLPRSHPLNVALGKLRHNWRRSRSKEA
                     AQPVSVPRS"
     gene            complement(4657..5007)
                     /locus_tag="VD788_12235"
     CDS             complement(4657..5007)
                     /locus_tag="VD788_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77076.1"
                     /translation="MEARIRTALTLYAFGVVGLFLLVTPWTGVWTQAVVGLLPTKLGQ
                     WVLGGWVRGTISGLGALNLAVAFQVAIELWKAMHRAADTAAARELLHEKNGGLAAAAG
                     EDGWEESTPWKEGR"
     gene            5068..6210
                     /locus_tag="VD788_12240"
     CDS             5068..6210
                     /locus_tag="VD788_12240"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXV77077.1"
                     /translation="MPSVPLLDLKAQYAPLRSALTEAVTRVVESQYFILGPEVEALER
                     EVATYTGAKHAIGCASGTDALVLSLAALDLEPEDEVITTPFSFFSTASCAYKVGARPA
                     FADIDPRTFNLDPERAEDAITERTRVLLPVHLFGQSAEMDGLLDIARRRELPLIEDAA
                     QALGASFRPRAATSPLRCGTMGRFGCYSFFPTKNLGCFGDGGMIVTSDDALAERVRVL
                     RVHGGRQMYHHRWVGWNSRLDALQAAVLRVKLPHLDAWSAGRARNADRYDRMLTEAGL
                     VEAGRVTLPYRGPGRDHIFNQYTLRVERRDELKAHLDASGIGNSIYYPVALHLQDCFR
                     ELGYGEGDFPHAEQACREVISLPVYPEIESAQLEAVVGRIVEFYRG"
BASE COUNT          991 a         2242 c         2134 g         1014 t
ORIGIN      
        1 acgaaccgac gacggagaat cggacgatga tcgacctttc gggaaagacg gcattggtca
       61 cgggaggctc gcgcggcatc ggcaaggcgt gctgcgagct gctggcacgg gccggggcca
      121 gggtcgcggt caactaccgc gtcgaacggc cgtgggccga gctgttggtg cagcgcatcg
      181 aggagcgcgg gggtcaagcg ttctgtctcg cggccgacgt ctcccggccg gacgaggcca
      241 acatgatggt cgacgagacg gtcgaccgct tcggccggct cgacgtgctg gtcaacaacg
      301 ccggcgtttg gcgcggcgat ccggtcgagg agatcagcga caacgagtgg cacgagacgg
      361 tcgcgatcaa cctgacgggg acgttccaca tgatccgctc cgcggtgccc tacatgaaat
      421 cgtcgggtgg cggccggatc atcaacatct cgagcaccgc gggccagcgc ggcgaggcgt
      481 ttcactgtca ctacgccgcg accaagggag cgatcatcgc gctgaccaag tcgctggcga
      541 gcgagctggc cgccgacggc atcttgacca actgcgtcgc gcccggttgg gtcgaaaccg
      601 acatgaccga gcaggtgctc gagcgcgatc gcgcgacgat cgtctccgcg ataccactcc
      661 gccgcgtcgc gcggccggag gagatcgcgg cggtcgtggt cttcctcgcg tccgacctgt
      721 cgagcttcat caacggcgaa gtcgtgaacg tgaacggcgg cagcgtgctg tgcggctgaa
      781 cctcggcagc actagttgtt ggcgcgacgt acggccacac tcagcaaccg ctcccgcggc
      841 tgatcgatcg aggcatcctc gtcgccgaag cgtctgaccc gcacgccgcg gaagccggcg
      901 cgacgtaagc gatattgcaa ctcgacctcg tggaacgcgc gcggcggacc gtccgcgcgc
      961 cctgccaggc gcatctcgcg cgcggcggga ccacgcggcg cggccggcac ggacgcgatc
     1021 atcaagccac cctcggccag cgacgcgtgg atcgcgctca ggcggcgatc gatttcggcc
     1081 aggtcgtctt gatcgagcga cgcgatgacg agcgccacgt cgaaaccgcg atcggtcgcc
     1141 tcgaggtcga acggcgcccg gcgccggtat tccacgttcg gctcgtcgcc ggcggggtcg
     1201 tgctcgccgg cgcggcgatc gatcgcgacc acgcgggcga aacactgcgc gaggaatctc
     1261 agccgcgccg gggtcgggcg gccgaagcag gccaccgttt tcgtctggcg gtcgcgcacg
     1321 gcggcgagga tctcgttgac gatttcgtag ggcgcccgat cgtcggcgcc gccgtcgcgg
     1381 tcgccgcgga tccacccgct ccaatcgtcg aaacctcgcg tcacggccac ctccgccggg
     1441 ggcgccgggc cccgtcgtca aatgtgagat ccgcgtgcgc ggggatcaac gtatcgcgat
     1501 gtatattcgc gagggccgcg gatgcggggc gggctcagga ctgcatgcgt tcgaggaact
     1561 cggcgttgct tcggtgcttg gccaggcgct cggtcagcac ctgcatcgct tcggccggct
     1621 tgagcggcgc cagggcccgc cgcagcagag tcaccatctt gtactcggtc gggctgaaca
     1681 gcttctcttc cttacgggtc ccgctcgagc tgatgtcgat cgacgggaag atgcggcgct
     1741 cgaacagcct ccggtccatc accacttcgg tgttgccggt gcccttgaac tcctggaaga
     1801 tcacctcgtc catgcgcgag ccggtgtcga cgagggccgt gccgatgacc gtcagcgacc
     1861 cgccgccgtg cgccttgcgc gcggcgccga agaagcgcct cggcttttcc atcgtgcgag
     1921 cgtcgatgcc gccactcagc acccgccccg agttgcgctg ctcggcgttg taggctcgcg
     1981 acagccgggt gatcgagtcg agcagcacga cgacgtcgcg cccgtactcg acgagacgct
     2041 tggcgcgctc gagcacgatc tccgcgatct gcacgtgcgt gcgcgccatc tcgtccgacg
     2101 acgacgcgat cacctcgccc cgcacgttgc ggcgcatgtc ggtgacctct tcggggcgct
     2161 cgtcgaccag cagcacgatc aggtggatct cggggtggtt gaccgagatg gcgtgggcga
     2221 tcttctggag cagcacggtc ttgccggcct tcggcggcgc gacgacgagg cagcgctggc
     2281 ccttgccgat cggactgatc agatcgacga cgcgggtcga aagctcctcg ccgacggtct
     2341 cgagtcggat gcgctcggtg ggatcttcgg cgacgaggtc cttgaacggg gtttgcgtgc
     2401 cgaactgctc ggggggctct ccgttgatcg agccgattcg aagcagccag ggcgcgcggc
     2461 cgtgctgctc ggcgggcgcg gcgagacccg acagctcgag accggtcgcg agaccctgct
     2521 cgcggatgat ctcgacgggg acgaccggat cggacgggtc ggcgttgaga tcgcgctcgg
     2581 tcgatcggag aaagccgtgc ccggcctcga cgatctgcag cacgccgacg acctcgacgc
     2641 tcggctcgac ctgcggcgcc cgggagatcg tcgcgaccca gttgccgccg ggctggcgct
     2701 ggtgatgatg acccccggtc ccgcgacctc cgcgccggcg gcctcccctc gaacggttcc
     2761 gactcctacg cctgccttct ttttgcgcgc tcatggctct ccgggctcgc taccgggttt
     2821 cggacccgtc ggtgcagacc aatcgctctt ctgcgtcgag gatctcggcg gacaacaaga
     2881 cgaccactgc gagcaagtcg tcgatgccgc cgctcgcggg cgtcggcagg atctgccaac
     2941 cgccctcgag cgcgcgcgcc gtcaaatacg cgcccggggt ttcccacccg ctcagatcgt
     3001 aaccggcttc ggccggcccc ctcggcgaga gacggaacag tcgtccgtcg cccattaaga
     3061 gatagcgcaa ttcgcgccgc tgcgccgccc cgccgagcga tgaggtgcag ccgagctcgg
     3121 tcccgccgtc gccgtcgaac agtcgaaacg gcccgttctc gtcacccgtc cgctccaaca
     3181 cccagcccga agccgtcccg ccggccgccg cccgccagcg ccccgcgtac ggctcgatcc
     3241 gcagcgcggg ccgaccggga cagtcgagga cgaaggagct cgggccgacg cgggtcaacc
     3301 cgaatggcgc gtgccgaggc atcattcggg agccggggtc gccgggtcga cttgcgcctc
     3361 gccttcccgt cgctcgcgca cgatctcgtg cgtgcgcagt gcgttggccg cggccgcgcg
     3421 cacgaactcg ttcttgtcct cgagcgcggc ctcgagtagc ggccggacgt cgggatcgcc
     3481 cgtcccgccg agtgcgcgag ccgcctggac tcggatctcg tagttggggt cctcgagcag
     3541 aagtcgggcc agctggggta tggccgcccc cggattcatt tcgcgcacgc cgcgcgcgca
     3601 agccagcctt acgtcgtcga tcgggtcgga caagccgcgc ccgagaccat cgaccgcctc
     3661 gtcgccgccg acccgggtca gtgcctccgc cgcgcggagc cgcacgaccg ggtcgctgtc
     3721 ctgcgtcagc cgcgcgacga ggacggcggc atcctcggac cctcccacgt cgccgaccag
     3781 tttggcggcg gtcgcccgaa cgaacccgtc ctcgtcggcc aggtggcgca gcgcgagctc
     3841 gcgcgcgtcg tcgacgccga gcgtgacgag cgcgttcaga gccgtggccc ggacgtcccg
     3901 gtcgggtgcg gcgagcagct cgcggatacg ctccacgttt tccgtcgtcg gctgggcctt
     3961 gaggtcatag atcgccgaaa tcttctcatt ccggttctcc tgacaggcca cgaggagcaa
     4021 caacatcgaa aacagcgatg aggattgccg aacaacacct ctcgttttgc tctttttttg
     4081 ttgcatgctc cgatccgtgt gcgtatcgga atgatcaaac agaaatatga ggtgtgatcg
     4141 agatgcctat tctatccgca gaaacccaat tcaagaaagg gctggcggcc ctagtggatc
     4201 acaactacaa ggacgcatcg gtcttttttc gccgtgccat cgaggccgac tcggcacgca
     4261 gcggcaagaa gcccgacctg cgttacctat cgtactacgg gctgagcctg tccaagtccg
     4321 gcggttcgac gaccgaggcg atccgcctct gccgcgcggc ggtcgccaaa caccggaacc
     4381 acccggtgtt gctgctcaac ctgggacggg tctatctcga ggccggcaag cactcgcacg
     4441 cactcgacgc gttcgaccgc gcgttgcgac tcgcgccgga caaccagacc ctggtgcgcg
     4501 agctgaagaa actggatcgt cgggccgcgc ccgtgctcgg catgctgccc cgctctcacc
     4561 cgttgaacgt cgcgctgggc aagctgcggc acaactggcg ccggagtcgt tccaaagagg
     4621 ccgcgcagcc cgtctcggtt ccccgcagtt gaccactcat ctcccctcct tccagggcgt
     4681 ggactcctcc catccatcct cgcccgcggc ggccgccagg ccgccgtttt tttcgtggag
     4741 cagttcacgt gcggcggcgg tgtccgccgc gcgatgcatc gccttccaga gctcgatcgc
     4801 gacctgaaag gccacggcga ggttgagcgc gccgagcccg ctgatcgtcc cccgtaccca
     4861 gccgccgagt acccattgcc ccagcttcgt cggcagcagg ccgacgaccg cctgggtcca
     4921 gacgccggtc caaggcgtga cgagcaggaa caggccgacc accccgaacg cgtacagcgt
     4981 caacgccgtg cggattctcg cttccaatcg gggtctccgg cggcatctta gcacccgccc
     5041 cgcgccatcc gcgctacaat ccgggccatg ccgtccgtgc cgttgctcga cctgaaggcg
     5101 cagtacgcac cgctgcggag cgcgttgacc gaggccgtca cccgtgtcgt cgagtcccag
     5161 tacttcatcc tgggacccga ggtcgaggcg ctcgaacgcg aagtggcgac ctacaccggc
     5221 gccaaacacg cgatcggttg cgcctcggga acggacgcgt tggtcctgtc gctcgccgcc
     5281 ctcgacctcg agccggagga cgaggtgatc acgacgccgt tctcgttctt ctcgaccgcg
     5341 tcgtgcgcgt acaaggtcgg ggcccggccg gcgttcgccg acatcgatcc acggaccttc
     5401 aatctcgacc ccgagcgcgc ggaggacgcg atcaccgagc gcacgcgcgt gctgcttccg
     5461 gtccatctgt tcggccagag cgccgagatg gacgggttgc tcgacatcgc gaggcggcgc
     5521 gagctaccgc tcatcgaaga cgcggcccaa gcgctcggcg cgagctttcg gccccgtgca
     5581 gccacgtccc cgcttcgctg cggaacgatg ggccgattcg gctgctactc gttcttcccg
     5641 acgaagaacc tcggctgctt cggcgacggc gggatgatcg tcacgtcgga cgacgcgctg
     5701 gccgagcggg tgcgcgtgct gcgtgtgcac ggcggccgcc agatgtacca ccatcgctgg
     5761 gtcggctgga acagccggct cgacgcgctg caggcggcgg tgctgcgtgt caagctgccc
     5821 cacctcgacg cgtggtccgc cggccgcgcg cgcaatgccg accgttacga ccggatgctg
     5881 accgaagcgg ggctggtcga agcgggtcgc gtgacgctgc cgtatcgcgg cccggggcgc
     5941 gaccacatct tcaatcagta cacgctgcgc gtcgagcgtc gcgacgagct gaaggcgcac
     6001 ctcgacgcga gcggcatcgg caattcgatc tactacccgg tcgcgctgca cctgcaggat
     6061 tgcttccgcg agctcggtta cggcgaaggc gacttccccc acgccgagca ggcctgccgc
     6121 gaagtgatct cgctgccggt ctaccccgag atcgagtcgg cgcagctcga agcggtggtc
     6181 ggccggatcg tcgagttcta ccgcgggtga gcgcgggcgc tcagaccggg ctcaccggca
     6241 cctcgccctc gtcgtcgtcg ctcaaccagt cgtagaggaa ccaacccgcg accagcacgg
     6301 cgaggacgac ccacgccttg gccggcgtgg tcagaccgct ccaccagcga ccgagcgacg
     6361 gatcgaccga cagcgcgcga t
//
LOCUS       DASPGN010000422         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1599297, whole genome shotgun sequence.
ACCESSION   DASPGN010000422 DASPGN010000000
VERSION     DASPGN010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599297"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VD788_12245"
     CDS             complement(<1..273)
                     /locus_tag="VD788_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77078.1"
                     /translation="MDPKELIHDWNVDADGDPPPEVVMLDDETLRDGLQSPSVVDPPI
                     ELKKRILHLMDELGIDTANLGLPGAGPRAVADVEGLCREIVVADLAI"
     gene            complement(345..1397)
                     /locus_tag="VD788_12250"
     CDS             complement(345..1397)
                     /locus_tag="VD788_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77079.1"
                     /translation="MSAGAAKQGSRRQFSLGWALGVSVVVHLLLFAGAGWLPLVPVRA
                     SSEPEETLLQFSFSEPVETAERSVPEGDVPFETAEPRPRPNTELTPPVDPGATPVPPR
                     PADADAQVEPGPETVLTDAERERQTAEDSSEVQAPALPRDDSEPLFPEDDRPLAEPDS
                     TVADERSRSFDLDRAIRDFGRVLDEARAENPVDPGSSPPRNVFVPDPAELDTTGYGLG
                     NLTFETADFDWSDYARAVYVQIWRAWHNRLLRMTNEFEKWAHQNQDPTLRHANQIRFV
                     IERSGQVSEILIEGPSGCVPLDDSATQALSEVILPPLPDAFPKEREVVHARFIAIGDI
                     RMMKPVLTRMKALNYF"
     gene            complement(1402..2520)
                     /locus_tag="VD788_12255"
     CDS             complement(1402..2520)
                     /locus_tag="VD788_12255"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXV77080.1"
                     /translation="MSQPTQRCPVRDFLERGVAARRMPAAAWSVACRERVVSRGAIGH
                     AVLEPTVEPSSDDTPFDLASLTKPLCTALLLVLAEADGSLALDAPISEFVEEMGGSAF
                     ADASLIELAVHAARLPAWRPLYLAGSDASSFAAQIARERPAVDDGESLYSDLGYVLLG
                     IAIERTAGATLDTLFAQRVARPLGLARLGFAAPGVRFADAAATERGNRYERVLAGRAG
                     RAFGWRRSVIRGEVHDGNAFALGGVAGHAGLFGPIAEVAALARELLDPHVLPLGGKAR
                     VRLFEPYRAGTSRTVGWILARGARAAAGILPDSAPGHTGFTGTSIWLDPDAGRSFVLL
                     TNRVHPEVRGGGFDPVRRGFHRVAAAVARSASGEQPLQ"
     gene            complement(2517..>3006)
                     /locus_tag="VD788_12260"
     CDS             complement(2517..>3006)
                     /locus_tag="VD788_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77081.1"
                     /translation="STDIDVVLDLMIHDLDLVVHMDGTEVVSVDAAGVAALTDKIDIA
                     NARIRFASGCVANLTASRISAESSRRIRLFQARTYLSCDTGKREVDRYRLALGDEGRP
                     EIRHERLPVEDGEPLRLELEAFVASVRERHTPPVDGRQARRAIELAQRVHRAIAASSP
                     AP"
BASE COUNT          453 a         1059 c         1048 g          446 t
ORIGIN      
        1 aatcgccagg tcggcgacga cgatctcgcg acagagcccc tcgacgtcgg ccaccgctcg
       61 cggtccggct cccggcaggc ccaggttcgc cgtgtcgatg cccagctcgt ccatcaggtg
      121 caggatgcgc ttcttcagct cgatcggggg atcgacgacc gacggcgact gcagtccgtc
      181 gcgcaacgtc tcgtcgtcga gcatcaccac ttccggcggc ggatcgccat ccgcgtcgac
      241 gttccagtcg tgaatcagct ctttcgggtc cattctcgtc cgctcctcgg ctggcgtcga
      301 tcgtaccggt ttccgcccga tgccgtcaac gcgcgccgcc gtcgtcagaa atagttgagc
      361 gccttcattc gggtcaacac cggcttcatc atccggatgt cgccgatcgc gatgaagcgc
      421 gcgtggacga cctcgcgctc cttggggaac gcgtccggca gcggcggcag gatcacttcg
      481 ctcagcgcct gggtcgccga gtcgtccagc ggcacgcaac cggacggccc ttcgatcaga
      541 atctccgaca cctgccccga ccgctcgatg acgaagcgta tctggttcgc gtgacgcagg
      601 gtcggatcct ggttctgatg ggcccacttc tcgaactcgt tcgtcatccg caacaggcga
      661 ttgtgccagg cacgccagat ctggacgtag accgctcgcg cgtagtcgct ccagtcgaaa
      721 tccgccgtct cgaaggtgag gttgcccaaa ccgtaacccg tggtgtcgag ctcggcgggg
      781 tcgggtacga agacgtttcg cggaggcgag cttccggggt cgaccgggtt ctccgcgcgc
      841 gcctcgtcga gcacgcgacc gaaatcgcga atcgcgcggt cgaggtcgaa cgaccgcgac
      901 cgctcgtcgg cgacggtgct gtcgggctcg gcgagcggcc gatcgtcctc ggggaacagc
      961 ggctcgctgt cgtctcgcgg cagcgcgggt gcctggacct ccgagctgtc ttcggcggtt
     1021 tggcgctctc gctccgcgtc ggtgaggacg gtctccggcc ccggctcgac ctgcgcgtcc
     1081 gcgtccgcgg gccggggtgg gacgggagtg gcgcctggat cgaccggcgg cgtcagctcc
     1141 gtgttcggtc gcgggcgcgg ctcggcggtc tcgaacggca cgtcgccctc gggtaccgaa
     1201 cgctcggcgg tctcgaccgg ctccgagaac gagaactgaa gcagcgtttc ctcgggttcg
     1261 ctcgacgctc gcacaggaac gagcggtaac cagccggccc ccgcgaacaa cagcaggtgg
     1321 acgacgacag agacgccgag cgcccagccg aggctgaact gacgtcggct tccctgcttt
     1381 gcggcgccgg cggacatgct ctcattgtag aggctgttcg ccgctcgcgc ttcgcgccac
     1441 ggccgcggcg acccgatgga aaccgcgccg aaccggatcg aaaccgccgc cgcgaacctc
     1501 gggatggacg cggttggtca gcagcacgaa cgaacgaccg gcgtccggat cgagccagat
     1561 cgacgtcccg gtgaacccgg tgtgcccggg agcggaatcg ggcaggatcc cggcggcggc
     1621 gcgcgccccc cgcgcgagga tccagccgac cgtccgcgac gttccggcgc ggtacggctc
     1681 gaacagccgc acacgggcct tcccgccgag cggtagcacg tgcgggtcga gcagttcgcg
     1741 cgccagcgcc gcgacctcgg cgatcgggcc gaacagcccg gcgtgacccg cgactccgcc
     1801 gagagcgaac gcgttcccgt cgtggacttc cccgcgaatc accgaccgcc gccagccgaa
     1861 tgcacgaccg gctcgaccgg cgagcacgcg ctcgtagcgg ttgccgcgct cggtcgccgc
     1921 cgcgtcggcg aaacgaacgc cgggggcggc gaaaccgagc ctggccagcc cgagcggtcg
     1981 tgccacgcgc tgcgcgaata gggtatcgag ggtcgcgccg gccgtccgct cgatggcgat
     2041 tcccagtagc acgtacccca ggtcggagta gaggctctcg ccgtcgtcga ccgccggccg
     2101 ctcgcgcgcg atctgcgcgg cgaagctgga cgcgtcgctg ccggccaggt agagcgggcg
     2161 ccaggcaggc aaccgtgcgg cgtggaccgc gagctcgatc agcgacgcgt cggcgaatgc
     2221 cgacccgccc atctcctcga cgaattccga gatcggtgcg tcgagcgcca gcgacccgtc
     2281 ggcctccgcc agcacgagca acagcgccgt gcacaacggc ttggtcaacg acgcgagatc
     2341 gaacggcgtg tcgtcggacg acggctcgac ggtcggctcg agcacggcgt ggccgatcgc
     2401 tccccggctg acgacccgct cccggcacgc gaccgaccag gctgcggccg gcatccggcg
     2461 cgcggccacg ccgcgttcga ggaagtcgcg aaccgggcag cgctgcgtcg gctggctcac
     2521 ggcgccggtg acgacgcggc gatcgcgcgg tgtacgcgct gggcgagttc gatcgcgcgc
     2581 cgggcctggc ggccgtcgac cggtggcgtg tggcgttcgc gtaccgaggc gacgaacgcc
     2641 tcgagctcga gacgaagcgg ctcgccgtcc tcgacgggga gtcgttcgtg acggatctcg
     2701 ggccgcccct cgtcaccgag cgccagccga tagcggtcca cctcccgctt gccggtgtcg
     2761 cacgacagat aggtgcgggc ctggaacaac cggattcggc gcgacgactc ggcggagatg
     2821 cggctggcgg tgaggttggc cacgcagccc gacgcgaagc ggatcctggc gttggcgatg
     2881 tcgatcttgt cggtcaatgc cgcaacgccc gccgcgtcga ccgaaacgac ctcggtaccg
     2941 tccatatgca ccacgaggtc gaggtcgtgg atcatcaagt cgagtaccac gtcgatgtcg
     3001 gtgctg
//
LOCUS       DASPGN010000423        20096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1130975, whole genome shotgun sequence.
ACCESSION   DASPGN010000423 DASPGN010000000
VERSION     DASPGN010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 20096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20096
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130975"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="VD788_12265"
     CDS             complement(<1..422)
                     /locus_tag="VD788_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HXV77082.1"
                     /translation="MARVYNWQIGRQMSYWYPEARPERQFAAVFDINKCIACQTCTLA
                     CKTTWTSGRGQEYMLWNNVESKPYGFYPLGWDVEILDRLGAQHWSADGSYQGRTLFEA
                     AEPGERMLGWLPDDLDYAHPNVGEDDCSGTVDRGAHFS"
     gene            complement(422..3883)
                     /locus_tag="VD788_12270"
     CDS             complement(422..3883)
                     /locus_tag="VD788_12270"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXV77083.1"
                     /translation="MKQNSLDRRSFLQFVAASGLGAFAASVNDAWGLGLPDNPLAAYP
                     DRGWEQVYRDLFEADSKFTFLCAPNDTHNCILDAHVRSGVITRIGPTMRYGEATDLYG
                     HRASHRWDPRVCQKGLALTRRFYGERRVRHPVVRAGYKRWIERGFPRGADGRPPAEYF
                     DRARDEWVRVTHDEAAEMVAKALVNIAETYSGEEGSRHLAAQGYDAETIDAMHGAGVQ
                     CLKFRGGMPLLGMTRVFGLYRMANSMALLDSKLRGVGPDQALGARGFDNYSWHTDLPP
                     GHPMVTGQQTVEFDLNAVEYAKTVLVWGMNWITTKMPDAHWLTEARMAGTRVVVIACE
                     YSATSTKADDAIVVRPGTTPALALGLAGEIIRNKLYDADYLRRHTDLPLLVRTDDLTY
                     LRARQVFGTAPAALWNNTRVVRGGETLPPPGAQREQLITEADREAWGDYVWWDRAANV
                     PRSITRDGVGKHSTIGDVLLEGEVEVPLHDGSKVRCRPVFDLIRDYTAHFDADTVEQL
                     TWAPAGAVRSLARDIAASPGTTLFAIGMGPNQFFNNDNKDRAIFLLAALTGNVGRLGG
                     NVGSYAGNYRVALFNGSPQYINEDPFDIELDATRSARVRQYWKAESAHYYNHEDHPLR
                     VGTRLLTGASHMPCPTKSMWFANANSILGNVKWHYNTVVNVLPRIEMIAVQEWWWSTS
                     CEWADIVFSVDSWAELKHPDVTASVTNPFITVFPRTPLARAFDTIGDIEVLALVGRKL
                     AAETGDRRFADLWHFVDAGRTDVYLQRIFDHSSNAKGYDFLDLEAKAKQGIPAFLLSR
                     TYPKAVGFEQVEDSVPWYTKTGRLEFYREEPEFIEAGENLPVHREPVDSTFYEPNVIV
                     AAPHEAIRPAGPTSYGVAEDDLSNDVRCGRNVVKSWQALERSPHPLAKDGYRFVFHTP
                     KYRHGAHTMPVDVDMIAVLFGPFGDLYRHDKRKPYVAEGYVDIHPADARELGIEDGDY
                     VWIDSDPSDRPFRGWQNDRRNYEFARLICRARYYPGTPRGVTRMWFNMYGATPGSVEG
                     ARSRADGLARNPRSPYQAMFRSGSHQSATRGWLKPTWMTDSLVRKGLFGQAIGKGFLP
                     DVHCPTGAPRESIVKITRAEPGGLDARGLWRPAERGLRPSYESEAMRRYLRGAFVAGA
                     GGDGEEG"
     gene            complement(3880..5559)
                     /locus_tag="VD788_12275"
     CDS             complement(3880..5559)
                     /locus_tag="VD788_12275"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV77084.1"
                     /translation="MRANKHLLLWTSLGTLLALSWAAYAEHGLQQWRSLQQDYRDALP
                     PAQAELFRVQLRQVHVPVLGATDRCVSCHVGMSPAETGIRGVARFAAHPDVVHDPSRF
                     GCTVCHGGQGLATVAEDAHGGVRHWPEPMIPQRHVWAGCGTCHAHLQVTNIAEMRAGR
                     DLFERYDCLACHKLEGRGGLARPGAAGGMEGPDLSRAGATGYDAEWYEGHLARRERET
                     SVAWRSSFGPMPAVERASIDTFLASRVGAPGLVEAKALFHSLGCRGCHPVHGVGGDDG
                     PDLTSVGQKDPGQLDFTHVPGERTLSNWLAEHFRAPAKIVPDSKMPYLGLTEQQIDAL
                     VFYMLSLRTTEFPEGFWPEDRVRVDHFGEREFAGDGATLYGTFCASCHGPQGQGMRYP
                     GMTAFPAIANPDFLRLASDDFLRATLTRGRPGRRMPAWGEKTGGLRPDEIETLVRFLR
                     ELGGVGQPETDGRPDRWAAGDPARGMTLWRENCASCHGTDGEGAEGPAIANRVLLDSA
                     SDSYFAETIRRGRRGTTMPAFSLPSPVRRTLDDDEIEALVAYIRDFEDGTR"
     gene            complement(5561..7126)
                     /locus_tag="VD788_12280"
     CDS             complement(5561..7126)
                     /locus_tag="VD788_12280"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXV77085.1"
                     /translation="MNAGAHDRAPARVTALVRFRDDLRAAPRRFYQTMFRSGAPASDR
                     TRSMFVFGNVFLHLHPARTHRWSLRWATTFGLGIASLAAFSITLVTGVLLMFYYKPYV
                     DVAYDSIKDIHFVVPTGRFIRNIHRWAGNVMVVTVILHMARVFYTAAYRRPREFNWVV
                     GILLLVLTLGLAFTGYLLPWDQLAYWAITIGANIAQSPREVTDVLGVTSWFDPGGLQK
                     LLLLGSNDVGEEALIRFYLLHVMILPLVLAGLLAVHFWRVRKDGGLTRPHDADERLGP
                     VRGIRPVFSELPQRTYHLAALVRGKSPAVGRGPEQTVPTMPHLLHAELAVFMLITLVC
                     VGLALVLDAPLKELANPAVPENPAKAPWYFLGLQELVAFSAFMGGIGIPAVVLAGLAL
                     IPYLDREREGTGLWFGGPGSLRLVGSSFAFGLAAVVAIEAFVIRFGWIRTWWPDTPQL
                     VITLINPGTILTLFYALYSITVIKRHDSTRAGALALFSCFLAGFVVLTMIGTYFRGPN
                     WEFYWSPGQWPGH"
     gene            complement(7123..7605)
                     /locus_tag="VD788_12285"
     CDS             complement(7123..7605)
                     /locus_tag="VD788_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXV77086.1"
                     /translation="MTTQSKVSRLDPEPLPRRDFLGWSALLAAGSALLFATLGMLRLP
                     KAAVLRSPSRKFRLTLPETLADGEAFVPAGRSIAVFRDRDGVYAISLVCTHLGCIVKP
                     TGRGFDCPCHGSRFAADGSVIKGPAPKALPWLAVSLAANTVTVDEDASVPAGTKELLA
                     "
     gene            complement(7602..9539)
                     /locus_tag="VD788_12290"
     CDS             complement(7602..9539)
                     /locus_tag="VD788_12290"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV77087.1"
                     /translation="MREEGRNHPSRLRVEVPGPGYHRRLIACQDACPVHTDARGYVRA
                     IAEGRFEEAYLIARGPNPFASICGRICGAPCEAACRRGRIPRTDDDGSFVGPDRPIAI
                     RALKRFACEQAGVEVRDPRDAIDAARSWVPRVCADAEEMAALLRALVSERLPSARGER
                     IAIIGSGPAGLSCAHDLALMGFRPVVYDSEPVAAGMLAVGVPEYRLPRELIRREVAVI
                     EALGVEIRCGVTVGRDVSFRRLRDEHAAVVLAVGAKRPRALGLPGERGPGVLGGVDFL
                     RAVALGEAPHVGRSAVVIGGGNVAYDVARTVLRQIAYDAARAAARLRDTRRVVLVSLE
                     TLEEMPADTVEIVEGDEEGIERLNGWGPVEVLRDARGRVTGLALRRCLRVYDDERRFA
                     PRFDDGQQRTVDCDTVLMATGQSTDLGFLADGGADVEQSRPGWPRVDPDTLATTGRGV
                     FVAGDLAHGTRLLIDAVASGKRAARSVYHHVCGSEIAMEVLETYMALPLYSRERRYDT
                     LSREQVPTLPPETRLADPSATVEIGYDATTACREASRCLDCGVTPVFDGGRCVLCGGC
                     VDVCPTLCLKLVSLADLAGDTEIDRLIDEGLGPSVDLDDNSAILKDEDHCIRCGLCAL
                     RCPVEAITMERIRFQTHWRTA"
     gene            complement(9520..9978)
                     /locus_tag="VD788_12295"
     CDS             complement(9520..9978)
                     /locus_tag="VD788_12295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXV77088.1"
                     /translation="MISRASRYALSVLRSLGQAEDERLSTAALADETGVPPSYLAKLL
                     TQLRKQGVVDAERGWGGGFRLRPEALDRPLRDIVVIFDGRERSADKACLLELTDCDED
                     NPCVLHPFWRQIRQALDELLTTQTIRDLAMTRTRDSLRERRRGPDEGRRS"
     gene            complement(10069..10578)
                     /locus_tag="VD788_12300"
     CDS             complement(10069..10578)
                     /locus_tag="VD788_12300"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXV77089.1"
                     /translation="MQLLLVVMLLIALAAGAVWAWGGREQTFRARIRIEATPEAVFRW
                     LTEPPLLARWIGGFVESRPLGDGRIRVGARSTEVIEERGRRTELQSEIVALDPGRRLA
                     VRLSSPFLTAENAFTLDASDDGATVVSQQLTMRYRGITRLFGPFLGGAVRAKLDADLE
                     RLRQGAETD"
     gene            complement(10747..12735)
                     /locus_tag="VD788_12305"
     CDS             complement(10747..12735)
                     /locus_tag="VD788_12305"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXV77090.1"
                     /translation="MRIRSRTIFTAGLVLAATAALAAEAQRPTKRVGAERARHGLTVE
                     QRIEQQRALNQQISPLVPAGVNRSPVSVEITRHDIEALSVDAPSGHAPLRIGVVKPLA
                     EGIGKPRGGAFTYGVTEKNADGSFVWALSVGSPGAQAIRVQLTGFSLPDRTEMFLLGA
                     NGQADGPYVGRGRNGDGEFWTRSIVGDSGTLVLKYDGTTPEAAHGEMAFTVAAIGHIR
                     GRKPRPVERSHDDWPCFTNVSCLVDATCGSTGPATPAEDAVAKMEWITGQFITTCTGG
                     LLADTDGSTQIPYFLTANHCVSSSVANLETFFNYTTDSCNGGCPDNLVTGGTPPAAST
                     VGVTLVATGSSQDFTLLTLNQAPPAGAVFLGWNNAPVAFSDGASLYRISNANFGPQVY
                     SEHTVDAGSPTCTGWSRGNRIYSVDEVGATMGGSSGSPVLNANGEVVGQLSGCCGFNC
                     GDECDAENNWTVDGAFAAYWNQVSDFLDPQGGGCTGDAECDDGQFCTGAESCVGGACQ
                     SSGNPCGAGETCNESTDSCDAPACDNDGTCEAGEDCNNCPNDCGSKVNGNPNSRYCCD
                     GDLPDCGDSRCSEGGFSCGDGGGTCSDDADCADGQFCNGDEICSGGSCQSGSDPCPGQ
                     GCDEGLNQCVTCGGNKDSCTTDSDCCSNACRGGTCRGN"
     gene            12885..14297
                     /locus_tag="VD788_12310"
     CDS             12885..14297
                     /locus_tag="VD788_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV77091.1"
                     /translation="MYKRRSRSITLTLAALLATTALAEDWTRFRGANGLGTSSSKGLP
                     AELGRETNKIWEIEVPFGRSSPVVAGDRIFLTAIEGEALVTLAIDRASGKTLWKKSVQ
                     PSRRDEYHHDTDSATTTPVTDGRNVYVFFQEFGIVSYDAQGEQRWTHPLGPFRNFYSI
                     AASPIVSGDLLYMFCDQAEGSFVVALDTKTGEQAWRRSRPGRLESYTTPILYPDEKKP
                     AALVIYGSAWIDAYELRTGETLWSVGEVGVGPVSSPVVAGDTIFVAAPSHGEHGWPPF
                     AGITGEFDKDGDGKLARDEVAEAWLIQHYGWLDRDGDGVISESDWTELGKEVMTEHWG
                     AYAIRLGGEKAEHLWNYRSNVSEIASPLVLDGVFYMVDKGILTSLDVKTGELIKRDRI
                     AEGSPKVYASPVAADGKIYLATLDGSVVVIRAAGEWETLSRVDLDDEIWATPAIADGR
                     LYLRTRHKLFSFGSSGGGAS"
     gene            14306..16132
                     /locus_tag="VD788_12315"
     CDS             14306..16132
                     /locus_tag="VD788_12315"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV77092.1"
                     /translation="MGRPTTIDGFTRRLLLVGLTLVAFGPLSAGDADGRLIITGVDVI
                     PLDAERVLRDQSVVVERGLITRIGPATDVAVDETAVVIDGAGKYLLPGLFDMHMHIGE
                     GQESDLTLYVANGVTTVQSMHGSPWHLALRERIAGGELLGPRLFTTGPTTATAGVDSP
                     ERAERFVDEQKEAGYDAIKQYGDGRDTMSRETYDRLIATAKARQIRVVGHAPRNIPFR
                     AVLEARQDSIDHAEEIYYTYGPIVDLYRPHLDFQFGRTSLEDYRAADPPFPDRRERLP
                     GVLAALATEMKRAGVALTPTLIAFETIRKQTTPEFDRLMQRPELSYVEPLTRASWGPR
                     SNRYRVGWASQLAEMDRVLGECLELQKALVKALDDVSVPVMAGTDAPLTFVFPGFSLH
                     GELEALVGAGLTPYRALRTATATPAEFLGIAGQAGTVQTGKRADLLLLDANPLADIRN
                     TRSIRGVVVRGRWIPRSEIDSLLSRIAESYEPRWNEIRIYEAMFEAGEIRKALATYDS
                     SKNGDERVASYLERTVNRIGYSYVRADDLARAIEVFTLNTEYFPDAFNTWDSLAEAHL
                     LAGDAALARRYYEKSLALNPDNSNALGQLDELRELPRTDEDR"
     gene            16129..16764
                     /locus_tag="VD788_12320"
     CDS             16129..16764
                     /locus_tag="VD788_12320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV77093.1"
                     /translation="MTRSRRSWQPYSERAAEFARRYESVSFERVHRPWSHLLADRGGR
                     ALDVGAGSGRDAVGLADRGFEVVAVEPAAGLRAEGRRRHPSPRIRWIDDALPELSAVR
                     NLELRFGLILLSAVWMHVPPAQRSGAVERLADLLAPAGLLVVTLRHGEPDRGRSMHPV
                     DGGGLTELCRAHALEPLAIPHPAADDPYGRIAVGWTIHVFRPSDDLRETER"
     gene            16788..17369
                     /locus_tag="VD788_12325"
     CDS             16788..17369
                     /locus_tag="VD788_12325"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HXV77094.1"
                     /translation="MFQNLAKYFFRGLLLVVPAALTVYVVYAVIVTLDGWINLSPLFG
                     RNVPGAGIVLTAALITLVGVLASNFATRWMFGVMDQLFTRLPLIKLLYTSLKDLIEAF
                     VGDKKRFDRPVLVRLDGSGELAFVGFLTRDELGPYGMPGKVAVYFPQSYNFAGNLLIV
                     PRERVTPLALDARTAMALIVSGGVSGDVPATGS"
     gene            complement(17366..18004)
                     /locus_tag="VD788_12330"
     CDS             complement(17366..18004)
                     /locus_tag="VD788_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77095.1"
                     /translation="MPRLRLALAALALGSARAALAAEGDVHLICPLVLAQQRIDVEEQ
                     RTEIALAEARLVAAERILGLVQRLHENDAIERIAYLAAKHDRDVAEVDLTRQRLIAKR
                     EQATLVQYETVCLPAGDGETERERAARREQAQRTFLQVECHRVGKDLAIAELDLAYFR
                     ELLDSARNLRANAAASVQDIIHAERDVEAARIRVEHHRLRVDRCIASGEAAG"
     gene            18118..18900
                     /locus_tag="VD788_12335"
     CDS             18118..18900
                     /locus_tag="VD788_12335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV77096.1"
                     /translation="MLLVKNKLARQVFGLLETANLLSLKLLFHNRSALRTYPGAVFRE
                     YMRLARRGKWVSMGVLELFPEVQQTRIVLEHMAGHGIYTPLDELAYLALITKALEPRS
                     IFEIGTFRGRTALNFALNSPADCRVFTIDLPPEGREQLSRDLGAADRGIVRASDTGVD
                     YRGKDVATKIEQLFGDSNAFDFSPYFGQIDLVFVDGAHDYRSVRNDSANARRMIRAGG
                     VIVWHDFANYGDYNDVTRAVLDLFPPSRIVQIENSQLAVYRS"
     gene            complement(18919..>20096)
                     /gene="ppk1"
                     /locus_tag="VD788_12340"
     CDS             complement(18919..>20096)
                     /gene="ppk1"
                     /locus_tag="VD788_12340"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HXV77097.1"
                     /translation="ELSFPPYSPQTPARLRQLDPAAPGAIFEEIGRGDILLHHPYHSF
                     ASSVLRFIESAARDPDVLAIQLTIYRTSSDSPIVAALADAARRGKQVAVLVEITARFD
                     EAPNIAWGAYLEKEGVHVAYGVEELKTHVKLALVVREEAGIVRRYLHVGTGNYHTGTA
                     RIYEDLGILTADPEICEDAAMVFAAITGATPLSGFKRMLVAPVTMRERFVELIRRETD
                     HARHGRPAGIEAKMNQLQDVEIIRELYRAGRAGVPITLNVRGLCCLRPGVPGLSENIR
                     VFGIIGRYLEHSRLYRFENGGDAEYFTGSADWMYRNLDRRVETVVPVKDPRICAELER
                     TLALYRRDNASAWDCGPDGVYTRRRPAVGEERRAVQEQMADAATPDDDEPAPRHDEG"
BASE COUNT         3143 a         6898 c         6931 g         3124 t
ORIGIN      
        1 aagctgaaat gggcaccccg gtcgacggtc ccactgcagt cgtcctcgcc gacgttcggg
       61 tgtgcgtagt ccaggtcgtc gggaagccac cccagcatgc gctcgcccgg ctccgccgct
      121 tcgaacagcg tccgaccctg atagctgccg tcggccgacc aatgttgcgc gccgagtcgg
      181 tccaggatct cgacgtccca gcccaacgga tagaagccgt acggcttcga ctcgacgttg
      241 ttccacagca tgtactcctg gccccggccc gaggtccagg tcgtcttgca ggccaacgtg
      301 caggtctgac acgcgatgca cttgttgatg tcgaacacag cggcgaactg tcgctcgggc
      361 cgcgcctcgg gataccagta agacatctgc cgaccgatct gccagttgta gacgcgtgcc
      421 atcagccctc ctccccgtcg ccgcccgcgc cggcgacgaa cgcgccgcgc aggtagcggc
      481 gcatcgcctc actctcgtag ctgggtctga gaccgcgttc ggcgggacgc cacaggcccc
      541 gcgcgtccag gccgcccggc tcggcccgcg tgatcttgac gatcgactca cgcggcgccc
      601 cggtcgggca gtgaacgtcg ggcaggaatc ccttgccgat cgcttggccg aacaggccct
      661 tcctgaccaa cgagtcggtc atccaggtcg gcttgagcca gccgcgcgtg gccgactggt
      721 gcgaaccgct gcgaaacatc gcctggtacg gcgagcgcgg gttgcgcgcc agcccgtcgg
      781 ctcgcgacct cgccccctcg accgatccgg gcgtcgcgcc gtacatgttg aaccacatgc
      841 gcgtcacccc gcgcggcgta ccggggtagt agcgcgcgcg gcagatcagc cgggcgaact
      901 cgtagttcct gcggtcgttt tgccagccgc ggaacggacg gtcgctcggg tcggagtcga
      961 tccagacgta gtcgccgtcc tcgataccca gctcacgcgc gtcggccggg tggatgtcga
     1021 cgtagccctc ggccacatag ggtttgcgct tgtcgtgccg atacaggtcg ccgaacgggc
     1081 cgaacagcac cgcgatcatg tcgacgtcta ccggcatggt gtgcgccccg tggcggtatt
     1141 tcggcgtgtg gaagacgaag cggtagccgt ccttggccag cgggtgcggc gagcgttcga
     1201 gtgcttgcca gctcttgacc acgttacgcc cgcaccgcac gtcgttcgac aagtcgtcct
     1261 cggccacgcc gtagctggtc ggtccggcgg ggcggatcgc ctcgtgcggc gcggcgacga
     1321 tcacgttcgg ctcgtagaac gtggagtcga ccggctcgcg atgtacgggc aggttctccc
     1381 cggcctcgat gaactccggc tcctcgcggt agaactcgag ccggccagtc ttggtgtacc
     1441 acgggaccga atcctccacc tgctcgaagc cgaccgcctt gggataggtc cggctcagca
     1501 ggaacgccgg gataccctgc ttcgccttgg cttcgagatc gaggaagtcg tatcccttgg
     1561 cgttcgacga gtgatcgaag atgcgttgca ggtagacgtc ggtgcggccg gcatcgacga
     1621 agtgccacag gtcggcgaaa cgccgatcgc cggtctcggc cgccagcttg cggccgacca
     1681 gcgccagcac ctcgatgtcg ccgatcgtgt cgaacgcccg cgcgagcggc gttcgcggga
     1741 agaccgtgat gaacgggttg gtcaccgagg cggtgacatc cgggtgcttc agctcggccc
     1801 acgagtccac cgagaagacg atgtcggccc attcgcacga ggtggaccac caccactcct
     1861 gcaccgcgat catctcgatc cgcggcagca cgttgaccac cgtgttgtag tgccacttga
     1921 cgttgcccag gatcgaattg gcgttggcga accacatcga cttggtcggg cagggcatgt
     1981 gcgatgcgcc ggtgagcagc ctggtgccca ccctcagcgg gtggtcctcg tggttgtagt
     2041 agtgcgccga ctcggctttc cagtactgtc tgacccgggc ggaccgggtc gcgtcgagct
     2101 cgatatcgaa cgggtcttcg ttgatgtact gcgggctgcc gttgaacagc gccacgcggt
     2161 agttgcccgc gtaggagccg acgttgccac cgaggcgacc gacgttgcca gtcagcgccg
     2221 ccagcaggaa gatcgcgcgg tccttgttgt cattgttgaa gaactggttg ggtcccatgc
     2281 cgatggcgaa cagcgtcgtg ccggggctgg cggcgatgtc gcgggcgagc gagcggacgg
     2341 cacccgccgg ggcccaggtc aactgttcga cggtgtcggc gtcgaaatgc gccgtgtagt
     2401 cgcggatcag gtcgaacacc ggccggcagc gcaccttgct tccatcgtgc aacggaacct
     2461 cgacctcgcc ttcgagcagc acgtcgccga tcgtcgagtg cttgccgact ccgtcgcgag
     2521 tgatcgagcg cgggacgttc gccgcgcggt cccaccatac gtagtcgccc caggcctcgc
     2581 ggtccgcctc ggtaatcaac tgctcccgct gtgcacccgg cgggggaagc gtctcgccgc
     2641 cccgaacgac ccgcgtgttg ttccacagcg cggccggtgc ggtgccgaac acctgtcggg
     2701 cgcgcaggta ggtcagatcg tccgtgcgga cgagaagcgg caggtcggtg tggcggcgca
     2761 ggtagtcggc gtcgtacagc ttgttgcgga tgatctcacc ggcaaggccg agggccagcg
     2821 ccggggttgt tcccgggcgc acgacaatcg catcgtccgc cttggtcgat gtcgccgagt
     2881 actcgcaggc gattacgacg acacgcgtgc ccgccatgcg ggcctcggtc aaccagtgcg
     2941 cgtcgggcat cttggtcgtg atccagttca taccccagac cagcacggtc ttggcatact
     3001 cgacggcgtt caggtcgaac tcgaccgtct gctggccggt gaccatcggg tggccgggcg
     3061 gaaggtcggt gtgccacgag tagttgtcga acccgcgcgc gcccaaggcc tggtccgggc
     3121 cgacgccgcg cagcttcgag tcgagcagcg ccatcgagtt ggccatgcga tacagaccga
     3181 acacgcgagt catgccgagc aacggcattc ctccgcgaaa cttcaaacac tggacgcccg
     3241 cgccgtgcat cgcgtcgatc gtctccgcgt cgtatccctg cgcggccaaa tgccggctgc
     3301 cttcctcgcc cgagtaggtc tcggcgatgt tgaccagcgc cttggccacc atctccgccg
     3361 cctcgtcgtg cgtgacccga acccactcgt cgcgtgcgcg gtcgaagtac tccgccggtg
     3421 gtctaccgtc cgcgcctcgc ggaaagcccc gttcgatcca gcgcttgtag ccggcgcgta
     3481 ccacggggtg acgcacgcgt cgctctccgt agaaccgccg cgtcaatgcc aggcctttct
     3541 ggcagacgcg tgggtcccaa cgatggctcg cgcggtggcc gtacagatcg gtggcttcgc
     3601 cgtagcgcat cgtcggaccg atgcgcgtga tcacccccga gcgcacgtgg gcgtcgagga
     3661 tgcagttgtg cgtgtcgttc ggcgcgcaca agaacgtgaa cttcgagtcc gcctcgaaca
     3721 ggtcgcggta gacctgctcc cagccgcggt cggggtacgc ggcgagcggg ttgtccggga
     3781 gcccgagacc ccaggcgtcg ttgaccgagg ccgcgaacgc accgagaccc gacgcggcga
     3841 cgaactgcag aaagctccgt cggtcgagac tgttttgctt catcgtgttc cgtcctcgaa
     3901 gtcgcggatg tacgcgacca gtgcttcgat ctcgtcatcg tcgagcgtgc ggcgcacggg
     3961 cgacggcagg gaaaacgccg gcatcgtcgt tccgcggcgc ccgcggcgga tcgtctcggc
     4021 aaaatagctg tcgctcgcgc tgtcgagcag gacgcggttg gcgatcgccg ggccctccgc
     4081 gccttcgccg tcggtgccgt ggcagctcgc gcagttctcg cgccacagcg tcataccgcg
     4141 cgcggggtcg cccgctgccc agcgatccgg gcgcccgtcg gtctccggct ggccgacgcc
     4201 acccaactcg cgcaggaagc gcacgagcgt ttcgatctcg tcggggcgca gcccgcccgt
     4261 cttctcgccc caggccggca tgcgccgccc gggacgcccg cgtgtcagcg tcgcacgcag
     4321 aaagtcatcc gacgccagac ggagaaagtc ggggttggcg atcgccggaa aggccgtcat
     4381 gcccgggtat cgcatgccct ggccctgcgg gccgtggcac gacgcgcaga aggtcccgta
     4441 cagggtcgcg ccatcgccgg cgaactcgcg ttcgccgaag tggtcgacgc ggacccgatc
     4501 ctcgggccag aacccctcgg gaaattcggt cgtccgcagc gacagcatgt agaagacgag
     4561 ggcgtcgatc tgctgttcgg tcagaccgag atagggcatc ttcgagtcgg gcacgatctt
     4621 ggccggagct cggaagtgct cggcgagcca gttcgacaga gtgcgctcgc cgggcacgtg
     4681 cgtgaaatcg agctggcccg ggtctttctg gccgaccgag gtcaggtcgg ggccgtcgtc
     4741 gccgccgacg ccgtggaccg ggtggcaacc gcggcagccg agtgagtgga acagcgcttt
     4801 cgcctcgacc agccccggtg cgccgacgcg cgaggcgagg aacgtatcga tcgacgcgcg
     4861 ttcgaccgcc ggcatcggac cgaacgagct gcgccatgcc accgacgtct cgcgctctcg
     4921 ccgcgccagg tggccctcgt accactccgc gtcgtaaccc gttgctccgg cgcgggacag
     4981 gtcgggaccc tccatgccac ccgccgcgcc gggtcgcgcc agccccccgc gtccctcgag
     5041 cttgtggcag gcgagacagt cgtagcgctc gaacagatcg cggcctgcgc gcatctcggc
     5101 gatgttcgtc acttgcaggt gtgcgtgaca ggtgccgcag ccggcccaaa cgtgacgctg
     5161 cgggatcatc ggctcgggcc agtgccgcac gccgccgtgg gcatcctcgg ccaccgtggc
     5221 gagcccttgg ccgccatggc agacggtaca gccgaagcgc gacggatcgt gcacgacgtc
     5281 ggggtgcgcc gcgaaccgcg ctacgccgcg gatgccggtc tcggcgggcg acatcccgac
     5341 gtggcagctc acgcagcggt cggtcgcgcc gagtaccgga acgtgaacct gacgcagctg
     5401 gacgcggaac agctccgcct gcgcgggcgg cagggcgtcg cggtagtctt gttggagcga
     5461 ccgccactgt tgcaggccgt gctcggcgta ggccgcccag gacaatgcca agagcgtgcc
     5521 gaggctggtc cacaacagca ggtgcttgtt ggcccgcatg tcagtgcccc ggccattgcc
     5581 cgggcgacca atagaactcc cagttcggcc cgcggaagta cgtgccgatc atcgtcagca
     5641 cgacgaagcc ggcgagaaaa cagctgaaca gggcgagcgc gccggcccgg gtcgagtcgt
     5701 ggcgcttgat gaccgtgatc gagtacagcg cgtagaacag cgtcaggatc gttcccgggt
     5761 tgatcagcgt gatgaccagt tgcggcgtgt cgggccacca ggtgcggatc cagccgaacc
     5821 ggatgacgaa cgcctcgatc gcgaccaccg cggcgagacc gaaggcgaac gacgaaccga
     5881 ccagccgcag actgcccggc ccgccgaacc agagccccgt cccctcgcgc tcgcgatcga
     5941 ggtagggaat cagcgccaag ccggcgagga cgacggccgg gataccgatg ccgcccatga
     6001 aggccgagaa ggcgaccagc tcctgcagcc cgagaaagta ccagggagcc ttggccgggt
     6061 tctcgggcac cgccgggttg gccagctcct tgagcggggc gtcgagcacc agcgccaacc
     6121 cgacacagac gagcgtgatc aacatgaaga cggccagctc ggcgtgcaac aggtgcggca
     6181 tcgtcggcac ggtctgttcg ggaccgcggc cgaccgccgg gctcttgccg cgcaccagcg
     6241 cggccagatg gtaggtgcgt tgtggcagct cgctgaacac ggggcgaatc ccacggactg
     6301 ggccgagtct ctcgtcggcg tcgtgcggtc gcgtcagtcc gccatctttg cggacacgcc
     6361 agaagtgcac cgccagcaga ccggcgagca ccagcggcag gatcatcacg tgcagcaggt
     6421 agaagcggat cagcgcctcc tcaccgacgt cgttcgagcc gagcagcagc agcttctgca
     6481 ggccgccggg gtcgaaccat gacgtcacgc cgagcacgtc ggtgacctcg cgaggcgatt
     6541 gggcgatgtt ggcgccgatc gtgatcgccc aataagccag ttggtcccag ggcagcaggt
     6601 agccggtgaa ggccagcccc agtgtcagca ccagcagcag gatgccgacg acccagttga
     6661 actcgcgcgg ccggcggtag gccgcggtgt agaacacacg cgccatgtgc aggatcaccg
     6721 tgacgaccat tacgttgccc gcccagcggt ggatgttgcg gatgaagcgg ccggtgggga
     6781 cgacgaagtg aatgtccttg atcgagtcgt aggccacgtc gacgtagggc ttgtagtaga
     6841 acatcagcag cacgccggtg accagcgtga tcgagaaggc ggcaaggctg gcgatgccga
     6901 gcccgaacgt ggtggcccaa cgcagcgacc agcggtgggt gcgagccgga tgcaagtgca
     6961 ggaagacgtt gccgaagacg aacatcgatc gcgtgcgatc gctggccggc gcacccgagc
     7021 ggaacatcgt ctgatagaac cggcgcggag cggcccgcag gtcgtcgcga aagcgcacca
     7081 acgcggtgac gcgcgccggc gctcggtcgt gcgccccggc gttcatgcca acagctcctt
     7141 ggtgcccgcg ggcacgctcg cgtcctcgtc gacggtgacg gtgtttgcgg cgagcgacac
     7201 ggcgagccac ggcagggcct tgggtgccgg gcccttgatc acgctgccat cggctgcgaa
     7261 gcgcgagccg tggcacgggc agtcgaatcc gcggccagtc ggcttgacga tgcagccgag
     7321 atgcgtgcag accagcgaga tcgcgtagac gccgtcccga tcgcggaaga ccgcgatcga
     7381 tcggccggcg ggcacgaatg cctcgccgtc ggccagtgtc tcgggcagcg tcagccggaa
     7441 tttccgcgac ggcgagcgca gcaccgcggc cttgggcagg cgcagcatgc cgagggtcgc
     7501 gaacagcagc gcggagccgg cggcgagcag cgcggaccac ccgagaaaat cccgacgcgg
     7561 aagcggttcg gggtcgagtc gcgagacctt cgattgggtc gtcatgccgt tctccaatgc
     7621 gtctgaaagc ggatccgctc catcgtgatc gcctcgaccg gacagcgcag cgcgcacagc
     7681 ccacaacgga tgcaatggtc ctcgtccttg agaatcgccg agttgtcgtc gaggtcgacg
     7741 gacggaccga ggccctcgtc gatcaaccgg tcgatctcgg tatcgcccgc cagatcggcg
     7801 agcgacacga gcttcaggca cagcgtggga cagacgtcga cgcagccgcc gcacagcacg
     7861 cagcgcccgc catcgaacac cggtgtcacg ccgcaatcga ggcagcgcga agcttcgcgg
     7921 caggcggtcg tcgcgtcata accgatctcg acggtggccg acgggtcggc gaggcgcgtc
     7981 tcgggcggca gcgtcgggac ctgctcgcgc gagagcgtgt cataacgtcg ctcgcgggaa
     8041 tacagcggca acgccatgta ggtctcgagt acctccatcg cgatttcgct gccgcagacg
     8101 tggtggtaga ccgagcgcgc ggcgcgcttg cccgacgcga ccgcgtcgat cagcaatcgg
     8161 gtgccgtggg ccaggtcgcc ggcgacgaaa acgccgcggc ccgtggtcgc gagcgtgtcg
     8221 gggtcgaccc gcggccagcc ggggcgcgat tgctcgacgt cggctccgcc gtccgcgaga
     8281 aagccgaggt cggtggactg acccgtggcc atcagcaccg tatcgcagtc gaccgtacgc
     8341 tgctggccgt cgtcgaagcg cggcgcgaag cggcgttcgt cgtcgtagac gcgcaggcag
     8401 cggcgcagcg ccaggccggt gacccggccc ctcgcgtcgc gcagcacctc gaccggaccc
     8461 cagccgttca ggcgttcgat gccctcctcg tcgccctcga cgatctcgac cgtgtcggcc
     8521 ggcatctcct ccagcgtttc gagcgagacg agtacgacac gtcgcgtgtc gcgaagccgc
     8581 gccgcggcgc gcgccgcgtc gtaggcgatc tgccgcagca ccgtgcgcgc cacgtcgtag
     8641 gccacgttgc cgccgccgat gacgactgcc gagcggccga cgtggggcgc ttcgccgagc
     8701 gccaccgccc gcaggaaatc gactccgccg agaacgccgg ggcctcgttc accgggcagg
     8761 ccgagggcac gcgggcgctt ggcaccgacg gcgagcacca ccgccgcatg ctcgtcgcgc
     8821 agccgacgga acgagacgtc ccgcccgact gtcacgccgc agcggatctc gacaccgagc
     8881 gcttcgatca ccgctacctc acgacggatc agctcgcgcg gcaagcggta ctcgggcacg
     8941 cctacggcaa gcatgcccgc ggcgaccggc tcggagtcgt agacgaccgg ccgaaagccc
     9001 atcagcgcca ggtcgtgcgc gcacgacagg ccggcggggc ccgaaccgat gatcgcgatc
     9061 cgctccccgc gcgcggacgg aaggcgctcg ctgacgaggg cgcgcagcaa cgcggccatt
     9121 tcctcggcgt cggcgcagac ccgcgggacc caggatcgtg cggcgtcgat cgcatcacgc
     9181 gggtcccgta cctcgacacc ggcctgttcg caggcgaagc gcttcaatgc acggatcgcg
     9241 atcggcctgt ccgggccgac gaagctgccg tcgtcgtcgg tgcgcggaat gcggccgcga
     9301 cggcaggcgg cttcacacgg cgctccgcag atgcgaccgc agatcgatgc gaacgggttc
     9361 ggcccgcgcg cgatcaggta cgcctcctcg aaccggcctt cggcgatggc gcgcacgtaa
     9421 ccgcgggcgt cggtgtgcac cgggcaggcg tcctgacagg cgatcaaccg tcgatggtac
     9481 ccgggcccgg gaacctcgac acgcaatctc gacggatggt tacgaccttc ttccctcatc
     9541 cgggcctcgg cgccgttcgc ggagcgaatc tctcgtgcgg gtcatcgcga gatcacggat
     9601 cgtctgcgtc gtcagcaact cgtcgagcgc ctgacggatc tgccgccaga acgggtgaag
     9661 cacgcaagga ttgtcctcgt cgcaatccgt cagctcgagc aggcaggcct tatcggcgct
     9721 ccgctcgcga ccgtcgaaga tcacgacgat gtcgcggagc ggtcggtcga gagcttcggg
     9781 tcgcaggcgg aagccgccgc cccacccccg ctccgcgtcg acgacgcctt gcttacgcag
     9841 ctgggtgagc agctttgcca ggtagctggg ggggacgccg gtctcgtcgg ccaatgccgc
     9901 cgtcgacagt cgttcgtctt cggcctggcc caggctgcgc aggacgctca gtgcgtaccg
     9961 tgaggcgcgg ctgatcatcg gactcctcaa gcgctctacg gatcagaata gcgattgaga
    10021 ttccgacaac aagccgcatg acgcaccgat agcggacccg atttgtcttc aatcggtctc
    10081 ggcgccctgc cgcagacgct cgagatcggc gtcgagtttg gcgcggaccg cgccgccgag
    10141 aaacggtccg aacagccggg tgatgccgcg gtagcgcatc gtcagctgct ggctcacgac
    10201 cgtcgctccg tcgtcgctcg cgtcgagcgt gaacgcgttc tcagccgtca ggaacgggct
    10261 cgacagccgc accgcgagcc gccgccccgg atcgagcgcg acgatctcgg actgaagctc
    10321 ggtcctgcgg ccacgctcct cgatcacctc ggtcgatcgc gcgccgacgc ggatccgccc
    10381 gtcgcccagc ggacgcgact cgacgaaccc gccgatccac cgcgcgagca gcggcggctc
    10441 cgtcagccag cgaaagaccg cctctggcgt ggcttcgatc cggatccggg cgcgaaaggt
    10501 ctgctcgcgc ccgccccagg cccagacggc gccggcggcg agcgcgatca gcagcatcac
    10561 gacgagcagg agttgcatgc gtctcactct ccagtccacc cgacttggcg ggacccgtca
    10621 cccatgtcga tgaagtccag ctgcaagagc tcggcctggc gcccgcgggg gtgcgccgcg
    10681 gcccgcgtcc caaaaaaagc gagccggcat cgccggctcg ccctcggatt cactgatggc
    10741 tccagctcag ttgccgcggc aggttccacc gcggcaggcg ttcgagcagc agtcggaatc
    10801 ggtcgtgcag ctgtccttgt tgccgccgca ggtcacgcac tggttgagac cttcgtcaca
    10861 cccctgaccc gggcacgggt cggaaccgct ctggcagctg ccgcccgagc agatctcgtc
    10921 gccgttgcag aactgaccgt cggcgcagtc ggcgtcgtcg gagcaggtgc cgccgccgtc
    10981 gccgcaggag aagccgccct ccgagcagcg cgaatcgccg cagtcgggga ggtcgccgtc
    11041 gcaacagtag cggctgttcg ggttgccgtt gaccttcgag ccgcagtcgt tcgggcagtt
    11101 gttgcagtcc tcgcccgcct cgcacgtgcc gtcgttgtcg caggccggcg cgtcgcacga
    11161 atcggtcgac tcgttgcagg tctcaccggc gccacacggg ttgccgctcg actgacacgc
    11221 gccgccgacg cagctttccg ctcccgtgca gaactggccg tcgtcgcact cggcatcgcc
    11281 ggtgcagccg ccgccctgcg gatcgaggaa atccgagacc tggttccaat aggccgcgaa
    11341 cgcgccgtcg accgtccagt tattctcggc atcgcattcg tcgccgcagt tgaagccgca
    11401 gcagcccgaa agctggccga cgacttcgcc gttcgcgttc aacaccggcg agccgctgct
    11461 gccgcccatc gtggcgccga cctcgtcgac gctgtagatc cgattgccgc ggctccagcc
    11521 ggtgcaggtc ggcgaaccgg cgtcgacggt gtgctcggag tagacctgcg ggccgaaatt
    11581 ggcgttgctg atgcgataga ggctggcgcc gtcgctgaac gcgaccggcg cgttgttcca
    11641 gccgaggaag accgcccccg cggggggagc ctggttcagc gtgagcaggg tgaagtcctg
    11701 gctcgagccg gtcgcgacca gcgtcacgcc gaccgtgctc gccgccggcg gcgtgccgcc
    11761 ggtgaccagg ttgtccgggc agccgccgtt gcacgagtcg gtcgtatagt tgaagaaggt
    11821 ctcgagattc gcgacgctcg acgagacaca gtggttcgcc gtcaggaagt agggaatctg
    11881 ggtcgagccg tcggtgtcgg ccaagagccc gccggtgcag gtggtgatga actgaccggt
    11941 gatccactcc atcttggcga cggcgtcctc ggccggcgtc gccgggccgg tgctgccgca
    12001 ggtcgcgtcg accagacaac tcacgttggt gaagcacggc caatcgtcgt ggctgcgctc
    12061 gaccgggcgc ggcttgcgac cgcggatgtg gccgatcgcc gcgacggtga aggccatctc
    12121 gccgtgggcc gcctcggggg tcgtgccgtc gtacttcaac accagcgtac cgctgtcgcc
    12181 gacgatcgag cgggtccaga actcgccgtc gccattgcgt ccgcgcccga cgtacggccc
    12241 gtcggcctgg ccattggcgc cgagcaggaa catctcggtc cggtccggta gcgagaaccc
    12301 ggtcagctgc acgcggatcg cctgcgcgcc gggagagccg acgctcagcg cccagacgaa
    12361 gctcccgtcg gcgttcttct cggtgacgcc gtaggtgaac gcaccgccgc gcggcttgcc
    12421 gatcccctcg gccaacggct tgacgacgcc gatccgcagc ggggcgtggc ccgacggcgc
    12481 gtcgaccgac agcgcctcga tgtcgtgccg agtgatctcg accgacaccg gagagcggtt
    12541 gacccccgcg ggaacgagtg gcgagatctg ctggttgaga gcacgttgct gctcgatcct
    12601 ctgctcgacg gtcagcccgt ggcgcgcacg ctccgctccg acccgtttgg tggggcgctg
    12661 tgcctcggcc gcgagcgcgg ccgtggccgc caggaccaac cccgccgtga agatcgtccg
    12721 tgaacggatt ctcatcgatt ccccctcctc ggttcgtggg acccgcggcg cgcgtgcgat
    12781 aacggcgcgc tccgaccccc gggttgacga ttggcggact ataccgtggt ttcgccggcc
    12841 ggggggaggt gggttagaat cgagcgagaa tcggggagat cgccatgtac aaacggagat
    12901 cccggtcgat cacgctgacg ctcgcggccc tgctcgcgac caccgccctc gccgaggact
    12961 ggacgcggtt ccgcggcgcc aacggtctcg gcacctcgtc ctcgaagggt ctgccggcgg
    13021 agctcggccg agagacgaac aagatctggg agatcgaagt gccgttcggc cgctcgtctc
    13081 cggtcgtcgc gggcgatcgc atcttcctca ccgcgatcga gggcgaggca ctggtcacgc
    13141 tggcgatcga tcgggccagc ggcaagacgc tgtggaagaa gagcgtgcag ccgagccgtc
    13201 gggacgagta ccatcacgac acggactccg cgacgaccac gccggtgacg gacggacgaa
    13261 acgtctacgt cttcttccag gagttcggca tcgtctcgta cgacgcgcag ggcgagcagc
    13321 gctggaccca tccgctggga ccgtttcgca acttctacag catcgccgca tcgccgatcg
    13381 tcagcggcga tttgctctac atgttctgcg atcaagccga aggatcgttc gtcgtcgcgc
    13441 tcgacacgaa gaccggcgag caggcatggc gtcgcagccg ccccggtcgc ctggagagct
    13501 acacgacgcc gatcctgtac cccgacgaga agaagcccgc tgcgctcgtg atctacggca
    13561 gcgcctggat cgatgcgtac gagcttcgaa ccggcgagac gctgtggtcg gtcggggagg
    13621 tcggcgtcgg gccggtatcg tcgccggtgg tcgccggcga cacgatcttc gtcgccgccc
    13681 cgagccacgg cgagcacggt tggccgccgt tcgccgggat cacgggcgag ttcgacaagg
    13741 acggcgacgg caagctcgcc cgcgacgagg tggccgaagc ctggttgatc cagcactacg
    13801 gctggctcga ccgggacggc gacggcgtga tcagcgagtc ggactggacc gagctcggca
    13861 aggaagtgat gaccgagcac tggggcgcct acgccatccg tctcggcggc gagaaggcgg
    13921 agcacctgtg gaactaccgt agcaacgtct ccgagatcgc gtcacccttg gtgctggacg
    13981 gcgtgttcta catggtcgac aagggaatcc tcaccagcct cgacgtcaag accggcgagc
    14041 tgatcaagcg cgaccggatt gccgaaggtt cccccaaggt ctacgcgtcg ccggtcgcgg
    14101 ccgacggcaa gatctacctg gcgacgctcg acggctcggt cgtcgtcatc cgcgccgcgg
    14161 gcgagtggga gacgctgtct cgcgtcgatc tcgacgacga gatctgggcg acgccggcaa
    14221 tcgccgacgg caggctctac ctccgcacgc gccacaagct gttcagcttc ggctcgagcg
    14281 gcgggggggc ctcgtagtcg gcacgatggg ccggccgacg acaatcgacg gtttcacgcg
    14341 ccgcttgctg ctggtcggcc tgacgctggt cgcgttcggc ccgctgagcg ccggcgatgc
    14401 cgatggtcgc ttgatcatca ccggcgtcga cgtcatcccg ctggatgccg aacgggtgct
    14461 gcgcgaccag tcggtggtcg tcgaacgggg tctgatcacc cggatcggtc cggcgaccga
    14521 cgtcgcggtc gacgagaccg cggtcgtgat cgacggcgcc ggcaagtacc tgctgcccgg
    14581 tctgttcgac atgcacatgc acatcggcga agggcaggag tcggatctga cgctctacgt
    14641 cgccaacggg gtcacgaccg ttcagagcat gcacggcagc ccctggcacc tcgcgctgcg
    14701 ggagaggatc gccggcggcg agctgctcgg gccgcggttg ttcaccaccg gaccgacgac
    14761 ggccaccgcg ggggtcgatt ccccggaacg ggccgagcgg ttcgtcgacg agcagaagga
    14821 ggccggttac gacgcgatca agcaatacgg cgacgggcgg gacacgatgt cgcgcgagac
    14881 ctacgatcgg ttgatcgcga cggccaaggc gcggcagatc cgcgtggtcg gccacgcgcc
    14941 gcgcaacatc ccgttccgcg ccgtcctcga agcgcggcag gactcgatcg accacgccga
    15001 ggagatctac tacacctacg gaccgatcgt cgacctctac cggccgcacc tcgattttca
    15061 gttcggccgg accagtctcg aggactatcg agcggctgat ccgccgttcc ccgaccgccg
    15121 agagcgcctg ccgggcgtgc tcgccgcgct cgcgacggaa atgaagcgcg cgggcgtggc
    15181 gttgaccccg acgctgatcg ccttcgagac gatccggaag cagacgactc ccgagttcga
    15241 ccggctgatg cagcgacccg agctgagcta cgtcgagccg ttgacgcgcg cgtcgtgggg
    15301 accgcgctcg aaccgctacc gcgtcggttg ggcctcgcag ctggcggaga tggaccgcgt
    15361 cctcggcgaa tgcctcgagc tgcagaaggc gctggtcaag gcgctcgacg acgtgtcggt
    15421 cccggtgatg gccggcaccg acgcgccgct gaccttcgtc ttcccgggct tctcgcttca
    15481 cggcgagctc gaagcgctgg tcggggcggg gttgacgccg taccgcgcgc tgcggacggc
    15541 gacggcgacg cccgccgagt tcctcggaat cgccggacag gccggcaccg tccagaccgg
    15601 caagcgtgcg gatctgttgt tgctcgacgc gaacccgctg gccgacatcc gcaacacgcg
    15661 ttcgatccgg ggggtcgtgg tccgcgggcg gtggatcccg cggagcgaga tcgactcgtt
    15721 gctttcgcgg atcgcggagt cgtacgagcc gcgctggaac gagatccgga tctacgaggc
    15781 gatgttcgag gcgggagaga tccgcaaggc gctcgcgacg tacgacagct cgaagaatgg
    15841 cgacgagcgg gtggccagct acctcgagcg caccgtcaac cgcatcggct actcgtacgt
    15901 tcgcgccgac gatctcgcac gggcgatcga agtgttcacg ctgaacacgg agtactttcc
    15961 ggacgcgttc aacacctggg actcgctggc cgaggctcac ctgctggcgg gcgacgccgc
    16021 gctcgcccgc aggtactacg agaagtcgct cgcgctcaat cccgacaact cgaacgccct
    16081 cgggcagctc gacgagctgc gcgagctccc tcggaccgac gaagacaggt gacccgatcg
    16141 cgccggagct ggcagcccta ctcggaacgc gcggcggagt tcgcgcggcg ctacgaatcg
    16201 gtctccttcg agcgggtcca ccggccgtgg tcgcacctgc tcgccgatcg gggcggccgg
    16261 gcgctcgacg tcggcgccgg ctcgggacgc gatgcggtcg ggctggccga tcgcggcttc
    16321 gaggtcgtcg cggtcgagcc ggccgccggg cttcgcgcgg aggggcggcg gcggcacccg
    16381 tcgccgcgga tccgctggat cgacgacgcg ctgcccgagc tgtccgccgt gcgcaacctc
    16441 gagttgaggt tcgggctgat cctgctcagc gcggtttgga tgcacgtgcc gcccgcgcaa
    16501 cggtcgggcg ccgtcgagcg gctggccgat ctgctcgcgc cggcaggtct gctcgtcgtc
    16561 acgctgcgac acggtgagcc ggaccgcggg cggtcgatgc acccggtcga cggcggcggg
    16621 ctgacggagt tgtgccgcgc gcacgcgctc gagccgcttg ccatcccgca ccccgcggcc
    16681 gacgatcctt acggccggat cgcggtcggc tggacgattc acgtcttccg cccgagcgac
    16741 gacctgcgcg aaactgagcg atgataggcc cgcaaggagg tggcgccttg ttccagaacc
    16801 tggccaagta cttctttcgc gggttgctgt tggtcgtacc cgccgcgctg acggtctacg
    16861 tcgtctacgc cgtcatcgtg acgctcgacg gctggatcaa tctgagtccg ctgttcgggc
    16921 ggaacgtccc cggcgccggc atcgtcctca ccgcggcgct gatcacgctg gtcggcgtgc
    16981 tggcgagcaa cttcgccacg cgttggatgt tcggcgtgat ggaccagctg ttcacgcggc
    17041 tgccgctgat caagctgctc tacacgtcgc tcaaggacct gatcgaagcg ttcgtcggcg
    17101 acaagaagcg gttcgaccgg ccggtgctgg tccggctcga cggcagcggc gagctggcct
    17161 tcgtcggctt cctcacgcgc gacgagctcg ggccttacgg catgcccggc aaggtcgcgg
    17221 tctacttccc gcagtcgtac aatttcgccg gcaacctgct gatcgtgccg cgcgagcggg
    17281 tgacgccgct ggcgctcgac gcccggaccg cgatggccct gatcgtctcg ggcggcgtct
    17341 cgggcgacgt gccggccacg ggctcctagc cggctgcctc gccggaggcg atacaccgat
    17401 cgacgcgcag ccggtgatgc tcgacgcgga tgcgcgccgc ctcgacgtcg cgctcggcgt
    17461 ggatgatgtc ctggaccgag gcggccgcgt tcgcccggag gtttcgcgcg ctgtcgagca
    17521 gctcgcggaa ataggcgagg tcgagttcgg cgatcgccag gtccttgccg acgcggtggc
    17581 actcgacctg gagaaacgtg cgctgcgcct gctcgcgccg tgccgcgcgt tcgcgctcgg
    17641 tctcgccgtc gcccgccggc aggcaaacgg tctcgtactg gacgagcgtc gcctgctcgc
    17701 gcttcgcgat cagccgctgg cgggtcagat ccacttcggc gacgtcgcga tcgtgcttcg
    17761 ccgcgagata cgcgatgcgc tcgatcgcat cgttctcgtg cagccgctgg accaggccga
    17821 gaatccgctc cgcggccacg agacgcgctt cggcgagggc gatctcggta cgctgctcct
    17881 cgacgtcgat ccgctgctgg gcgagcacga gtggacagat cagatgcacg tcgccctcgg
    17941 cggcgagtgc tgcccgcgcc gagccgagcg cgagggcggc gagcgccagg cgaagtcgcg
    18001 gcatggaacc tccgaccacg atgatagcgg cccccgaccg cgacgccacg cgcggaccga
    18061 tcccgccggt cgaatccggc ctgggccgtt gcgggtggct cgcgtaagat ggccggcatg
    18121 ctgctcgtca agaacaagct ggcccgtcag gtcttcggcc tgctcgagac cgccaacctg
    18181 ctgtcgctca agctgctgtt tcacaaccgg tcggcgcttc gcacctatcc cggggcggtc
    18241 tttcgcgagt acatgcgcct cgcgcggcgg ggtaagtggg tgtcgatggg tgtgttggag
    18301 ctgttcccgg aagtccagca gacccgcatc gtgctcgagc acatggccgg ccacgggatc
    18361 tacacgccgc tcgacgagct ggcctacctg gcgctgatca ccaaggcgct cgagccgcgg
    18421 agcatcttcg agatcggcac cttccgcggg cgcaccgcgc tcaacttcgc gctcaacagt
    18481 ccggccgact gccgcgtctt caccatcgat ctcccgcccg aaggacgaga gcagctgtcg
    18541 cgcgatctcg gcgccgccga ccgcggcatc gtgcgggcga gcgataccgg tgtcgactat
    18601 cgcggcaagg acgtcgcgac caagatcgag cagctgttcg gcgactcgaa cgctttcgac
    18661 ttctcgccgt atttcggcca gatcgatctg gtcttcgtcg acggcgcgca cgactaccgt
    18721 tcggtgcgca acgacagcgc caacgcgcgg cgcatgatcc gcgccggcgg cgtcatcgtc
    18781 tggcacgact tcgccaacta cggcgactac aacgacgtga cccgggccgt gctcgatctg
    18841 ttcccgccgt cgcgcatcgt ccagatcgag aactcgcagc tcgcggtcta ccggtcgtga
    18901 gcggcgagcc gccgccgctc agccctcgtc gtgccgcggc gccggctcgt cgtcgtccgg
    18961 ggtcgccgca tccgccatct gctcctggac cgcccggcgc tcctcgccga ccgccggccg
    19021 gcgacgcgtg tagacgccgt cggggccgca gtcccaggcc gacgcgttgt cgcgccggta
    19081 gagcgcgagg gtccgctcga gctccgcgca gatccgcggg tccttgaccg gcacgaccgt
    19141 ctcgacccgc cggtcgaggt tgcggtacat ccagtcggcg gagccggtga agtactcggc
    19201 gtcgccgccg ttctcgaagc ggtagagccg gctgtgctcg aggtagcggc cgatgatgcc
    19261 gaagacccgg atgttctccg acagtcccgg cacgcccggt cgcaagcagc acagtccgcg
    19321 cacgttgagc gtgatcggca cgccggcccg tcccgcccgg tacagctcgc ggatgatctc
    19381 gacgtcctga agctggttca tcttggcttc gatccccgcg ggacgaccgt ggcgcgcgtg
    19441 gtcggtctcc cgccggatca gctcgacgaa tcgttcgcgc atcgtcaccg gtgcgaccag
    19501 catccgcttg aaaccgctga gcggggtggc ccccgtgatc gcggcgaaga ccatcgcggc
    19561 gtcctcgcag atctcggggt cggcggtcag gatgccgagg tcctcgtaga tccgcgccgt
    19621 cccggtgtgg tagttcccgg tgccgacgtg caggtagcgg cggacgatcc cggcctcctc
    19681 gcgcacgacg agcgcgagct tgacgtgggt cttcagctcc tcgacgccgt aggcgacgtg
    19741 cacgccctcc ttctccagat aggcgcccca ggcgatgttc ggcgcctcgt cgaagcgggc
    19801 ggtgatctcg acgagcaccg cgacctgctt gccgcggcgg gcggcgtcgg cgagcgcggc
    19861 gacgatcggc gagtcgctcg acgtgcggta gatcgtcagc tggatcgcga gcacgtcggg
    19921 atcccgcgcg gccgactcga tgaagcgcag caccgagctg gcgaagctgt ggtacgggtg
    19981 gtgcagcagg atgtcgcctc gaccgatctc ctcgaagatc gcgccgggcg ccgccgggtc
    20041 gagctgccgt agccgcgcgg gggtctgcgg cgagtagggc gggaacgaca gctcgg
//
LOCUS       DASPGN010000424         5729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_780218, whole genome shotgun sequence.
ACCESSION   DASPGN010000424 DASPGN010000000
VERSION     DASPGN010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5729
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780218"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="VD788_12345"
     CDS             <1..1225
                     /locus_tag="VD788_12345"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXV77098.1"
                     /translation="ALGNGLTGVFLQDTDDTIVANNVVSGNSEWGIVHVDTAPAVPLV
                     PAQIIRNVVGMNATGSAGLGNTFSGIALQEVAGTTVADNLVSNNGQAGIALFRGTRTA
                     SVVRNIVGADTTGTLAFGNGNEGIFLFDARDNAIGDGSAAGGNRILFNGLQGVAVAET
                     AANLARGNVVSGNEIRANSALGIDLFAGGVAGVTANDPLDPDNGPNGLQNFPIVDMAE
                     VGLVETLISGRLHSKTGTAYDVELFSNRTAGCDPSNHGEGQVLLERLGVVTDAAGNAP
                     IVTAVTGVALGDPITATATNAEGTSEFSMCRAAVPCATLMAFPESIVGSAFDSVDWLV
                     PTDVAYAIFTLNALPAFAVSESGVLFNATSVALSGVNPAPGTGFGVLVREWGCGSWQT
                     TIGAEPNRDSTLIAP"
     gene            complement(1274..2836)
                     /gene="hybB"
                     /locus_tag="VD788_12350"
     CDS             complement(1274..2836)
                     /gene="hybB"
                     /locus_tag="VD788_12350"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HXV77099.1"
                     /translation="MTIEARKFTFWKVILLAILCGGAYAAIVRYAFGLGAATHLSDSF
                     PWGLWIGFDVLVGVGLAAGGFVIAATVHVFRIERYEAIARPTILTAFLGYLLVCVALL
                     FDLGMPHRIWHPLIMWNPHSVMFEIGWCVMLYTTVLALEFSPLVFERFNLRRPLKLVR
                     MLYIPLVIAGVLLSTLHQSSLGTLYVIAPGKLHGLWYSPLLPVFFFISAIAGGLAMTI
                     FESFISRRAFGRELEGDLLRGLGRVIVVVLAVLAVWRLQDLALRGNFGLIFRLTPESV
                     MFWGEFILGIAIPLALFALPRVRASESGLLLASMLTIMGFVVNRLNVAITGMISSSGV
                     TYVPSWMEFAVTIAIVAAGFALFAAAVRYLAVFPEGPAAASATVDPLARLATIGRPVF
                     GGTVLFTLWALLLAGGVAVAYSLDRGATPAGPRARPATSEPSRALPADASLPAPFTFP
                     SSEESPGPVVFDHRSHVDPHEAACDTCHRSMFRINEAGVPLTGALDYERIHQGDLCAS
                     CHATDDDCGLCH"
     gene            complement(2826..3629)
                     /locus_tag="VD788_12355"
     CDS             complement(2826..3629)
                     /locus_tag="VD788_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXV77100.1"
                     /translation="MKPAILNDLTRCIGCEACVWACREVNELPVDERADRLSSGALSA
                     VERRGDVFVRRQCMHCLEPACVSVCPVAALHKTPEGPVVYDASRCIGCRYCMIGCPFG
                     IPRYEWDDPTPKVQKCTMCFARRLQHGEQPACTAACPTGAALFGDRDALIEEAQRRIR
                     EEPDRYVDHVYGVQEAGGTSVIYLSAVPFADLGFETTVANDPYPRLTWQVLSKIPNVV
                     SVAGVLLFGIWWLTGRKELIQRVHDGRISLEQAMREMPSFSPPEHDHDN"
     gene            complement(3631..4368)
                     /locus_tag="VD788_12360"
     CDS             complement(3631..4368)
                     /locus_tag="VD788_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77101.1"
                     /translation="MVVLLVLSMILLFLTADYLVQRSHLRRLAREATVASPSLAGPAD
                     TERVPDEVCLTPGHVWMRRDPTGSVRVGVDPVLISLLGGVEHAYTVEPGHELAAGGPL
                     VMLRRGSRALRIRSPLAGRVAEVNERLVTTPGQLASDPYRDGWLYRIEPSSSSDSLRG
                     GLAGETARRFLERERVRLRDLLEALTSRPEQAVAHAADGGLPAGDAVDHLDDRQWERL
                     IATFFHDDSRHEGTLVAFRAPRDTAGV"
     gene            complement(4527..5201)
                     /locus_tag="VD788_12365"
     CDS             complement(4527..5201)
                     /locus_tag="VD788_12365"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV77102.1"
                     /translation="MSEEHSIRVLIVDDHSVVRRGLRALLATEPGIEVVAEAVDGEDA
                     VSRAADSRPDVVVMDLVLPSMDGVEAIRRIKLRDPDARILVLTGFGSDDKLFPALKAG
                     ALGYLLKDASPEELVRAIRATARGLSSLNQQIARRLVAELANEGPDRPPSRDALTQRE
                     IEVLRLLARGMSNDDIGDRLHISDATVRTHVSNILFKLRLDNRTQAALYALKEGVASL
                     NDIRST"
     gene            complement(5198..>5729)
                     /locus_tag="VD788_12370"
     CDS             complement(5198..>5729)
                     /locus_tag="VD788_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXV77103.1"
                     /translation="NQPEAAVEHCRELRQASLKALRELRHMIFELRPPDLEKLGLVAA
                     LETRLATVEGRAGLKTELHCEGMTDLPFAVSDGLYRIANEAFANSVRHAQASRISLRL
                     ERREREVVLEILDDGTGFDIDAGRSGGGFGLSCMEERAKRMGGTFAVQTERGRGTRIR
                     VSVPVADPAASAAEPR"
BASE COUNT          969 a         1980 c         1934 g          846 t
ORIGIN      
        1 agcgctgggg aacggactga ccggtgtgtt tctccaggac accgacgata cgatcgtcgc
       61 gaacaacgtc gtctcgggca actccgagtg gggcatcgtc cacgtcgata ccgctccggc
      121 cgttccgctg gttccggccc aaatcatccg caacgtcgtc gggatgaacg cgaccggctc
      181 ggccgggctc ggcaacacct tcagcggcat cgcgttacag gaggttgcgg gcacgacggt
      241 cgccgacaac ctggtctcga acaacggcca ggccggtatc gccctgttcc gtggtacgcg
      301 caccgcgagc gtcgtgcgca acatcgtcgg cgccgacacg acggggacgc tggctttcgg
      361 caacggcaac gaaggcatct tcctgttcga cgccagggac aacgcgatcg gcgacggatc
      421 ggcggccggc ggcaaccgca tcctgttcaa cggcctgcag ggggtcgccg tcgccgagac
      481 cgcggcgaat ctcgcacggg gcaacgtcgt cagcgggaac gaaattcgcg cgaacagcgc
      541 gctcggcatc gacctgttcg ccggcggtgt cgcgggcgtc accgccaacg atcctctcga
      601 ccccgacaac ggcccgaacg gactccagaa cttcccgatc gtcgacatgg ccgaggtcgg
      661 cctggtcgaa acgctgatca gcgggcggtt gcacagcaag accggcacgg cgtacgacgt
      721 cgagctgttc tcgaaccgta cggccgggtg cgatccgtcg aatcacggcg agggtcaagt
      781 gcttctcgag cgcctgggcg tcgtgaccga cgccgcgggg aacgcgccga tcgtcaccgc
      841 ggtgacgggt gtcgccctcg gcgacccgat taccgcgacg gcgaccaacg ccgagggcac
      901 ctccgagttc tcgatgtgtc gcgccgccgt tccgtgcgcg acgctgatgg cgtttcccga
      961 gtcgatcgtc gggtccgcgt tcgattcggt cgattggctg gttccgaccg acgtggccta
     1021 cgcgatcttc acgctcaacg cgctgccggc cttcgccgtt agcgagtcgg gcgtgctgtt
     1081 caacgcaact tccgtcgcgc tgtccggggt caacccggct ccgggcaccg gcttcggcgt
     1141 gctggtgcgg gagtggggct gcggcagttg gcagacgacg atcggtgcag agccgaaccg
     1201 ggactcgacg ctgatcgcgc cgtagcacgt cgggtcggcc gccggcggag cgcgcggctc
     1261 cgccggcgag gtgttagtgg cacagcccgc agtcgtcgtc ggtcgcgtgg cacgaggcgc
     1321 acaggtcgcc ctggtggatc cgttcgtagt cgagcgcccc ggtcagcggg acgcccgctt
     1381 cgttgatgcg gaacatcgag cggtggcagg tgtcgcaagc cgcctcgtgc ggatcgacgt
     1441 gactccggtg gtcgaacacg accggccccg ggctctcctc actgctgggg aaggtgaacg
     1501 gcgcgggcaa cgaagcgtcg gccgggagcg cgcgcgacgg ttcactcgtc gccggtcgcg
     1561 ctcgaggtcc ggccggcgtc gcgccacgat cgaggctgta ggcgaccgcg acgccgccgg
     1621 ccaacagcag cgcccacagc gtgaacagca ccgtcccgcc gaagaccggt cggccgatcg
     1681 tcgccagccg cgccagcggg tcgaccgtcg cgctggccgc cgccggtccc tcggggaaga
     1741 ccgcgaggta gcgcacggcc gccgcgaaca gcgcgaagcc ggcggcgacg atcgcgatcg
     1801 tcaccgcgaa ctccatccag ctcggcacgt aggtcacgcc cgagctggag atcatgccgg
     1861 tgatcgccac gttcaagcga ttgacgacga agcccatgat cgtcagcatc gaggccagca
     1921 gcagcccgct ttcgctcgcg cgcacgcgcg ggagcgcgaa caacgcgagc gggatcgcga
     1981 tgccgaggat gaactcgccc cagaacatca cgctctcggg ggtcaggcgg aagatcaggc
     2041 cgaagttccc gcgcagggcc aggtcttgca gccgccagac cgcgagcacc gccagcacca
     2101 ccacgatcac ccgacccaat ccgcgcagca ggtcgccctc gagctctcgc ccgaaagccc
     2161 gccggctgat gaacgactcg aagatcgtca tcgccagccc gccggcgatc gcgctgatga
     2221 agaagaagac cggcagcagc gggctgtacc acaggccgtg cagcttgccg ggtgcgatca
     2281 cgtacagtgt gccgagcgac gattgatgca gcgtcgacag cagcacgccg gcgatgacga
     2341 gcgggatgta gagcatgcgg accagcttca gcggccgcct gaggttgaat cgctcgaaca
     2401 ccagcgggct gaactcgagc gccaacaccg tggtgtagag catgacgcac cagccgatct
     2461 cgaacatgac cgagtgcgga ttccacatga tcagcggatg ccagatccgg tgtggcatgc
     2521 ccaggtcgaa caacaaggcg acgcagacca gcaggtagcc gaggaacgcg gtcaggatcg
     2581 tcgggcgcgc gatcgcttcg taccgctcga tgcggaagac gtgcacggtc gccgcgatga
     2641 cgaagccgcc ggcggcgagg ccgacgccga ccagcacgtc gaagccgatc cacaggcccc
     2701 aggggaacga gtccgacaga tgggtcgccg cacccagccc gaaggcgtag cgcacgatcg
     2761 ccgcgtacgc gccgccgcac aggatcgcca gcaggatgac tttccagaag gtgaacttgc
     2821 gggcctcaat tgtcatggtc gtgctccggc ggcgaaaacg acggcatctc tcgcatcgcc
     2881 tgctcgagcg agatccggcc gtcgtgcacc cgctgaatga gttccttgcg accggtcagc
     2941 caccagattc cgaacaacag gacaccggcg acgctgacca cgttggggat cttcgacagc
     3001 acctgccagg tcagacgcgg atacgggtcg ttggccaccg tggtctcgaa gcccagatcg
     3061 gcgaacggca ccgcggacag ataaatcacc gaggttccac ccgcctcctg cacgccgtag
     3121 acgtgatcga cgtatcggtc gggttcctcg cggatacgac gctgcgcctc ctcgatcaac
     3181 gcatcgcggt cgccgaacag cgccgcgccg gtcgggcaag ccgcggtgca tgccggctgc
     3241 tcgccgtgct ggagccgccg agcgaagcac atcgtgcatt tctgcacctt cggcgtcggg
     3301 tcgtcccatt cgtagcgcgg gatgccgaac ggacagccga tcatgcagta gcggcagccg
     3361 atgcaacgcg aagcgtcata gaccaccggc ccttcgggcg tcttgtgcaa agcggcgacc
     3421 ggacagaccg acacgcacgc cggctcgagg cagtgcatgc actggcgccg gacgaaaacg
     3481 tcgccgcgcc gctcgacggc gctcaaggca ccggaggaca agcggtcggc gcgctcgtcg
     3541 accggcaatt cgttgacttc acgacaggcc cacacgcagg cctcgcagcc gatgcagcgt
     3601 gtcaggtcgt tcaggatggc gggtttcatc tcagactcca gccgtgtcgc gcggagcacg
     3661 gaaagcgacg agggtgccct cgtgcctcga atcgtcgtgg aagaaagtcg cgatcagccg
     3721 ttcccactgg cgatcgtcga ggtggtcgac ggcgtcgccg gccggcaacc cgccgtccgc
     3781 cgcatgggcg acggcctgct cgggacggct ggtcagtgcc tcgagcagat cgcgcaggcg
     3841 cacgcgttcg cgttcgagga agcggcgggc ggtctctccg gccagcccgc cgcgcagcga
     3901 gtcggacgac gacgacggtt cgatgcggta cagccagccg tcccggtacg ggtcgctcgc
     3961 cagctgcccc ggcgtcgtga cgagccgctc gttgacctcg gccacgcggc ccgcgagcgg
     4021 cgagcggatt ctgagcgcac gtgacccgcg ccgcagcatc accagcgggc cgcccgcggc
     4081 cagctcgtgc cccggctcga cggtgtacgc gtgctcgacg ccaccgagca aggagatcaa
     4141 caccgggtcg accccgacgc ggaccgaccc cgtcggatcg cgccgcatcc agacgtgacc
     4201 cggtgtgagg cagacctcgt ccgggacccg ctcggtgtcg gccggccccg ccagcgaggg
     4261 cgacgcgacc gtcgcttccc tggccagccg cctcaggtgc gaccgctgga ccagatagtc
     4321 cgcggtgaga aacaggagga tcatcgacag gaccagcagc actaccatgg tgtccctcct
     4381 ttccgttcca tgaccagcat cgttcggacg accccggcgc ggcatcggag gaaagaccgc
     4441 ttttgcctca tctcgccggt ggagggagcg agcggcgtcg cgcgatattc ggcgtagttg
     4501 cggggtacga ggaaaggcgg agtgggctac gtggaacgga tgtcgttcaa gcttgccacg
     4561 ccttccttca gcgcgtacag cgcggcctgc gtccggttgt ccagtcgcag cttgaacagg
     4621 atgttgctga cgtgcgtccg caccgtcgcg tcgctgatgt ggagtctgtc gccgatgtcg
     4681 tcgttgctca tcccgcgagc cagcaggcgc agcacctcga tctcgcgctg ggtcagcgcg
     4741 tcgcgcgagg gcgggcgatc cggtccctcg ttcgccagct cggcgaccaa ccgacgcgcg
     4801 atctgttgat tcaacgacga gagaccgcgg gcggtcgcgc ggatcgcacg gaccagttcc
     4861 tcggggctgg catctttcaa caggtagccc agcgcgccgg ccttgagcgc gggaaacagc
     4921 ttgtcgtcgc tgccgaagcc ggtcaacacg aggatccgcg cgtcgggatc gcggagtttg
     4981 atccgtcgga tcgcctcgac cccgtccatc gacggcagaa cgagatccat cacgaccacg
     5041 tccggtcggg agtccgccgc ccggctgacg gcgtcctcgc cgtcgaccgc ttcggcgacg
     5101 acctcgatgc cgggttcggt ggcgagcagc gcgcgcagac cccggcgcac gaccgaatgg
     5161 tcgtcgacga tcagtacgcg gatcgaatgt tcttcgctca tcgcggctcc gctgccgacg
     5221 cggccgggtc ggcgaccggc acgctcaccc ggattcgcgt cccgcgaccg cgctcggtct
     5281 ggacggcgaa ggttccgccc atccgtttcg cccgctcctc catacagctc agcccgaacc
     5341 ccccgccgct tcgtccggcg tcgatgtcga agcccgtgcc gtcgtcgagg atctcgagca
     5401 cgacctcccg ctcgcgtcgt tcgaggcgca aactgatcct ggacgcctgc gcgtgtcgca
     5461 ccgagttggc gaaggcctcg ttcgcgatgc ggtacagccc gtccgagacg gcgaacggca
     5521 ggtcggtcat tccctcgcag tgcagctccg tcttcagtcc ggcgcggcct tcgaccgtgg
     5581 ccaaccgagt ctcgagcgca gcgaccagcc ccagcttctc gaggtcgggt ggacgcagct
     5641 cgaagatcat gtgccgcagc tcccgcaacg ccttcagcga ggcctgccgc agctcgcgac
     5701 agtgctcgac ggcggcctcg ggctggttc
//
LOCUS       DASPGN010000425         4477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_313165, whole genome shotgun sequence.
ACCESSION   DASPGN010000425 DASPGN010000000
VERSION     DASPGN010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313165"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VD788_12375"
     CDS             <1..137
                     /locus_tag="VD788_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HXV77104.1"
                     /translation="LKATIRDYIRTNNEDPKPFVWTKTADEILASVARFCQRTSDSGH
                     "
     gene            362..2077
                     /gene="pilB"
                     /locus_tag="VD788_12380"
     CDS             362..2077
                     /gene="pilB"
                     /locus_tag="VD788_12380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HXV77105.1"
                     /translation="MAVKLGEMLVKAGVISASQLEEALEAQRGNGEKLGFNLIKLGYV
                     REDDITQLLSEQFGVPSINLRHFEIDETVINLIPSEVAQKYLILPVNRTGATLTISMA
                     DPTNVFAMDDIKFMTGYNVEPVVASEIAIREAIEKYYGSQHALELKKVMDEMAEAEAE
                     ALEVLEEDEELDIASLEAATEEAPVVKLVNIILTDSIKKSASDIHIEPYERDFRVRFR
                     IDGVLYEIMHPPMKLRDAITSRLKIMAKLDISEKRLPQDGRIKVKMKLGGKTKEMDYR
                     VSCLPTLFGEKIVLRLLDKDNLMLDMTKLGFESESLQKFERAILRPYGMVLVTGPTGS
                     GKTNTLYSSMNRVNTPETNIMTAEDPVEFNLHGINQVQTKEQIGLNFATALRSFLRQD
                     PNIVLVGEIRDFETAEIAVKAALTGHLVLSTLHTNDAPSTINRLMNMGIEPFLVATSV
                     NLICAQRLVRRICRECVEEINMPTQALVDVGFSPEDAASSKLKKGRGCPSCNNTGYRG
                     RVGLYEVLEVSEEIRELILSGASAIELRRKAIEEGMISLRLSGVMKIKQGVTTVEEVV
                     RETVL"
     gene            2098..3222
                     /locus_tag="VD788_12385"
     CDS             2098..3222
                     /locus_tag="VD788_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXV77106.1"
                     /translation="MSVTMHQLLKTLVDQGGTDLHIATNSPPQIRIHGKMVPLQSPPM
                     TPAETKSIIYSVLSDNQKHRLEENFELDFSFGVKGLARFRANIFFQRGAVGGAFRLIP
                     WEIRSFRELGLPEVVATLCDKPRGLILVTGPTGSGKSTTLASMIDKVNSERHEHIVTI
                     EDPIEYLHAHKKCLVNQRELHADTQTFSKALRAVLRQDPDVVLIGEMRDLETIESALR
                     IAETGHLTFATLHTNSAAQTINRIIDVFPPHQQSQIRAQLSLVLEGILCQALLPRANG
                     GGRALAMEILVPNSAIRNLIREDKVHQIYSAMQVGQAKYGMQTFNQSLATLVMKKQIT
                     RELALSMSSNAEELREMLERGATGLQPTTAGAGARRQVRR"
     gene            3256..4458
                     /locus_tag="VD788_12390"
     CDS             3256..4458
                     /locus_tag="VD788_12390"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXV77107.1"
                     /translation="MPSYSWKGRTRGGKIQEGVLAAESKEAAIATLRKQQIIVTAVTE
                     RGKEFALPRLGGGISNKQIAVFTRQFSVMIDAGLPLVQGLEILGSQQDNRTFQKILFQ
                     VRQDVETGSTLADSLRKHPKAFDDLYCNMVAAGEAGGILDTILQRLAQYIEKIVKLRA
                     AIRSAMVYPIAVITIAIGVVWVILWKVIPTFATLFEGLGASLPLPTRITIAVSNFIGA
                     WWWLMIGSVIVGIVLLVRYHKTYKGRRHLDGLLLRLPVLGMVLKKIAVARFCRTLGTL
                     VSSGVPILDGLEITARTSGNAIVEDAIMATRKSVEEGKTIAEPLKASDVFPPMVIQMV
                     AVGEQTGALETMLSKIADFYEDEVDEATANLLALLEPVMICFLGVVIGGIVISMYLPM
                     FDLINKIG"
BASE COUNT          905 a         1389 c         1421 g          762 t
ORIGIN      
        1 agctgaaggc cacgatccgc gactacattc gcacgaacaa cgaagatccc aagccatttg
       61 tctggacgaa gactgctgat gaaatcctgg catccgtcgc ccgattttgt cagcgaactt
      121 cggactcagg acactagcgg ctgtcgacgc cgaatgaccg ccgttttgac cgattccgcc
      181 acccgtatca caatctttgt caccgcgtgc cgttccttga caataatttt caaggaacct
      241 agattcgcca cgattcgaga ctccgcgatc agccgaggcg tctcgcgcgg ccgggccggg
      301 atgaaagcgc agcgctgccg aagagtccgg ggaaccaccg agtaaggggg cgacagaacc
      361 tatggccgtc aaactgggcg agatgctggt caaggccggt gtgatcagcg cctcgcagct
      421 cgaagaggcg ctcgaggccc aacgtggcaa cggtgagaag ctcggcttca acctgatcaa
      481 gctcggctac gttcgcgagg acgacatcac ccaactgctc tccgagcagt tcggcgtccc
      541 gtcgatcaac ttgcggcact tcgagatcga cgaaaccgta atcaacctga tcccgtcgga
      601 ggttgcgcag aagtacctga tcctgccggt caaccgcacc ggtgcgacgc tgacgatctc
      661 gatggccgat ccgaccaacg tgttcgccat ggacgacatc aagttcatga cgggctacaa
      721 cgtcgagccg gtcgtcgcca gcgagatcgc gattcgcgag gcgatcgaga agtactacgg
      781 ctcccagcac gcgctcgagc tgaagaaggt catggacgag atggccgagg cggaggccga
      841 ggcgctcgag gtgctggagg aagacgaaga gctcgacatc gcgtcgctcg aggcggccac
      901 cgaagaggct ccggtcgtca agctcgtcaa tatcatcttg accgactcga tcaagaaaag
      961 cgcttccgac atccatatcg agccctacga gcgcgacttt cgcgtccgct tccgcatcga
     1021 cggggtgttg tacgagatca tgcacccgcc gatgaagctg cgcgacgcga tcaccagccg
     1081 gctgaagatc atggccaagc tcgatatctc cgagaagcgg ctgccgcagg acgggcggat
     1141 caaggtcaag atgaagctcg gcggcaaaac caaggagatg gactaccgcg tctcatgcct
     1201 gccgacgttg ttcggcgaaa agatcgtgct gcgcttgctc gacaaagaca acctgatgct
     1261 cgacatgacg aagctcgggt tcgagtccga gtcgctgcag aagttcgagc gggcgatcct
     1321 ccggccgtac ggcatggtgc tggtgaccgg ccccaccggc tcgggcaaga ccaacacgct
     1381 gtactcgtcg atgaaccggg tcaacacgcc cgagaccaac atcatgaccg cggaggaccc
     1441 ggtcgagttc aacctccacg gcatcaacca ggtgcagacc aaagagcaga tcgggctcaa
     1501 cttcgccacg gcgctgcgtt cgttcctgcg gcaagacccg aatatcgtgc tggtcggtga
     1561 gatccgcgac ttcgagaccg ccgagatcgc ggtcaaggcg gcgctgaccg gccacctcgt
     1621 gctgtcgacg ctgcatacca acgacgcgcc gtccacgatc aaccgcctga tgaacatggg
     1681 catcgaaccg ttcctcgtcg ccacctccgt caatctgatc tgcgcccagc gtctggtgcg
     1741 tcggatctgc cgggagtgtg tcgaagagat caacatgccg acccaggcgc tggtcgacgt
     1801 cggcttctca cccgaggacg ccgcgagctc gaagctgaag aagggccgag gttgtcccag
     1861 ctgcaacaac accggctacc gtgggcgcgt cgggttgtac gaggtgctcg aagtgtccga
     1921 ggagattcgc gagctgattc tgtcgggagc gtccgcgatc gagctgcgcc gcaaggcgat
     1981 cgaagaaggg atgatctcgc tgagactctc gggagtaatg aagatcaagc agggcgtgac
     2041 gaccgtcgaa gaggtcgtgc gcgagacggt cctgtaggac ctcgcggaga tgcaagcatg
     2101 tcggtaacga tgcaccagct gctgaagacg ctcgtcgacc agggcgggac ggaccttcat
     2161 atcgccacca attcgccgcc ccagatccgc atccacggca agatggtccc actgcagtcc
     2221 ccgccgatga cgccggccga gacgaagagc atcatctaca gcgtgctgtc cgacaatcag
     2281 aagcaccggc tcgaggagaa cttcgagctc gacttctcct tcggggtcaa ggggttggcg
     2341 cggttccgcg ccaacatttt cttccagcgc ggcgccgtcg gcggggcgtt ccggctgatc
     2401 ccgtgggaga tccgctcgtt ccgcgagttg ggtctgccgg aggtggtggc gacgctgtgc
     2461 gacaagccgc gcggcttgat tctcgtgacc ggaccgacgg gctcgggcaa gtcgacgacc
     2521 ctggcgtcga tgatcgacaa ggtcaacagc gagcgtcacg agcacatcgt gacgatcgag
     2581 gacccgatcg agtacttgca cgcacacaag aagtgcctgg tcaaccagcg cgagctgcac
     2641 gccgacaccc agaccttcag caaggcgctg cgcgcggtgt tgcggcagga tcccgacgtc
     2701 gtgctgatcg gcgagatgcg cgacctggag acgatcgaat cggcgctgcg catcgccgag
     2761 accggccact tgaccttcgc cacgctgcac acgaactcgg cggcgcagac gatcaaccgc
     2821 atcatcgacg tcttcccgcc gcaccagcag tcgcagatcc gggcccagct gtccctcgtc
     2881 ctcgagggga tcctgtgcca ggcgctgctg ccacgagcca acggcggcgg gcgcgcgctg
     2941 gcgatggaga tcctcgttcc gaactcggcc attcgcaacc tgatccgcga ggacaaggtc
     3001 catcagatct attcggcgat gcaggtcggc caggcgaagt acgggatgca gacgttcaac
     3061 cagtcgttgg ccacgttggt gatgaagaag cagatcaccc gcgagctggc gctgtcgatg
     3121 agctcgaacg ccgaggagtt gcgcgagatg ctcgagcgcg gcgccaccgg tctgcagccg
     3181 acgaccgccg gtgccggtgc ccggcggcaa gtgcgtcgct gatttcacgc ggtcagacaa
     3241 gaaggagtcg gaacgatgcc cagttacagt tggaaggggc gaacccgcgg cggcaagatc
     3301 caggaagggg tgctggccgc cgaaagcaag gaggcggcga tcgcgacgct gcgcaagcag
     3361 cagatcatcg tgacggccgt gaccgagcgg ggcaaggagt tcgcgctccc tcgtctcggc
     3421 ggtgggatca gcaacaaaca gatcgcggtc ttcacccgcc agttctcggt gatgatcgac
     3481 gccggcctcc cgctcgtcca gggtctggag atcctcggct cgcaacagga caaccgcacg
     3541 ttccagaaga tcctgtttca agtacggcag gacgtcgaga ccggttcgac gctggccgac
     3601 tcgctgcgca agcaccccaa ggcgttcgac gacctgtact gcaacatggt cgccgccggt
     3661 gaggccggcg gtattctcga cacgatcctg cagcgtctcg cgcagtacat cgaaaagatc
     3721 gtcaagctgc gcgcggcgat tcgctccgcg atggtctacc cgatcgcggt catcacgatc
     3781 gcgatcggcg tcgtctgggt cattctgtgg aaggtcattc caaccttcgc cacgttgttc
     3841 gaaggcctcg gtgcgagcct gccgctgccg acccgcatca cgatcgccgt gtcgaacttc
     3901 atcggcgcct ggtggtggct gatgatcggc agcgtcatcg tcggcatcgt cctgttggtg
     3961 cgctaccaca agacgtacaa ggggcgacgg catctcgacg ggttgctgct gcggctgccg
     4021 gtgctgggga tggtgctgaa gaagatcgcg gtcgcgcgct tctgccgcac gttgggcacc
     4081 ctggtctcct cgggcgttcc gatcctcgac ggccttgaaa tcaccgcacg aacgtcgggc
     4141 aatgcgatcg tcgaggatgc gatcatggcg acccgcaaga gcgtcgagga gggcaagacg
     4201 atcgccgagc cgctgaaggc gtcggacgtc ttcccgccga tggtcataca gatggtcgcc
     4261 gtcggcgaac agacgggtgc cctcgagacg atgctgtcga agatcgccga tttctacgag
     4321 gacgaggtcg acgaggcgac agccaacctg ctcgccttgc tcgaaccggt gatgatctgc
     4381 ttcctgggtg tggtcatcgg cggcatcgtg atctcgatgt acctgccgat gttcgacctg
     4441 atcaacaaga tcggctgatc gcacgaggga cgcgccg
//
LOCUS       DASPGN010000426         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1600417, whole genome shotgun sequence.
ACCESSION   DASPGN010000426 DASPGN010000000
VERSION     DASPGN010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1600417"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VD788_12395"
     CDS             <1..483
                     /locus_tag="VD788_12395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77108.1"
                     /translation="NRVLGRQSGAVFAEAAVEVAGRLGMRGVVVTGSNYSGPLPDSTE
                     VIAHRGLPGAELAGLLASARFGIVGGGDLLQQAIAMHTPSVTAPAAPDQPRRIAAFER
                     VDLCITARLERLAETTVEAVRDGRIDALRERLASSDTANGLMLGVERIEALAGSRPSG
                     "
     gene            495..1817
                     /locus_tag="VD788_12400"
     CDS             495..1817
                     /locus_tag="VD788_12400"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HXV77109.1"
                     /translation="MSQGTITDAAAPAIGGRLAGLPAVVTAGFATTVGMWAAGYLCRL
                     PAILLPSPFVLVLLLACLFTGGLLLGRLSGGGATGGAAAGLVSAALNLLVLGSLLGGA
                     EADRLVPSALWWIPGSILASTALGALGSAIGSARPVARAAPFNWLNAFVWVAVAATAL
                     LLAAGGLVTSAEAGLAVDDWPTSFGYNMFLYPFSKMTGGIYYEHAHRLLGALVGLTTV
                     VLASFVQATEPRRWVRAWAWVGVPTVIVQGVLGGIRVTERNLALAMAHGVLAQLFFAG
                     LVALAVFTSDRWRGPIEPTVRRGARTDLRLGPTLVAVTVVQLVLGASQRHFQQLLMAH
                     LTLGLLVVLPLALAVGSRAWGLATGRDGLLRRLGRLLVAVVLVQVVLGFGALVVTLAG
                     SDGAIGPASLLVFPTAHQWLGAVLLAVTTALVCWNSRCLDEPGAGRTG"
     gene            2055..>3680
                     /locus_tag="VD788_12405"
     CDS             2055..>3680
                     /locus_tag="VD788_12405"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HXV77110.1"
                     /translation="MTTSTRRDLVRRHRDVFVALLALAIWAPGASADVVIHEIMYHPL
                     SNADSDEFVEIFNTGGSPVDISNWCFDGVDFCFPAGGTTIGAGDFLVIANDAVGFQTT
                     YGFPPDHVFTPATASVLDDAGERLALIDQGFVIVDELLFTDEPPWPVTPDGLGPSLEV
                     IDATLDNSTPRNWRASLAAGGTPGAANSVAATGLPPWIDQIQHTQDVLPLDPVVVTAR
                     VVAATTVDLTYKIDFGGEVTLQMLDNGSSGDGGAGDGVYGAAIPGQPIGTLVRYRITA
                     TGPTGTIRYPRADDTVTYDGTAVVDPALSSELPILHWFIDPIDYQEALDHRLTDETEP
                     AVLFYDGTLYDAVETRIRGASARQWPKPPWKFFTPQGHNFAAPGLIVNDVDTFNIQSG
                     YADKSYTREVLAWETVGEAGLPAGRVFPIRVEQNGQFFGLFTFLEAPDSDFVVRNRLD
                     VRGARYKAFENLRMHMTIDEIVPLYEKKSRLEEDHSDLWQLITQVNQPATQQIHDWLA
                     DNIDIPTVVNYIAAMSIVHNNDHLNKNYFLYRDT"
BASE COUNT          506 a         1281 c         1263 g          630 t
ORIGIN      
        1 aatcgcgtgc tgggtcgtca gagcggcgcg gtcttcgcgg aggctgcggt cgaggtggcc
       61 ggtcggctcg ggatgcgcgg cgtcgtcgtc accggctcga actactccgg tccgctaccc
      121 gattcgacgg aggtgatcgc ccatcgcggc ctgcccggag ccgagctggc gggtctgctc
      181 gcgtcggcgc gcttcggcat cgtcggtggc ggcgacctgc tgcaacaggc gatcgcgatg
      241 cacacccctt cggtcaccgc cccggcggcg cccgaccaac cgcggcggat cgcggccttc
      301 gagcgcgtcg acctgtgcat caccgcccgg ctcgagcgac tcgccgagac gaccgtcgag
      361 gcggtgcgcg acggacggat cgatgccttg cgcgagcggc tcgcgtcgag cgacacggcc
      421 aacggtctga tgctcggcgt ggagcggatc gaggcgctcg ccggatcgcg cccgagtggc
      481 tagaatgacg ccgggtgagc cagggtacga tcaccgatgc cgccgcgccg gccatcggcg
      541 gccgcctcgc cggtctgccc gcggtcgtga ccgcgggttt cgcgacgacg gtcgggatgt
      601 gggccgccgg ttacctctgc cgcttgccgg cgatcctgtt accgagcccg ttcgtgctcg
      661 tactgctcct cgcctgcctg ttcaccggcg ggctcttgct cggtcgcctc tcgggcgggg
      721 gagcgaccgg aggcgcggcg gcgggtctgg tttcggccgc gctcaacctc ttggttctcg
      781 gtagcctgct cggtggcgcg gaggccgacc gcctggtccc gtcggcgttg tggtggatcc
      841 cgggctcgat cctcgcgtcg accgcccttg gcgcgctcgg ctcggcgatc ggctccgctc
      901 ggcccgtcgc gcgcgcggca ccgttcaact ggctgaacgc cttcgtctgg gtcgccgtcg
      961 cggcgaccgc actgctgctc gccgccggtg gtctcgtaac cagcgccgag gccgggctgg
     1021 cggtcgacga ctggccgacg tcgttcggct acaacatgtt cctctacccg ttctccaaga
     1081 tgacgggcgg gatctactac gagcacgcgc accgcctgct gggagcgctc gtcgggctga
     1141 ccaccgtcgt ccttgcgtcg ttcgtccagg cgaccgagcc gagacgatgg gtgcgggcgt
     1201 gggcctgggt cggggtgccg acggtgatcg tccaaggggt gctgggcggg attcgggtaa
     1261 ccgagcgcaa cctggcgctg gcgatggccc acggcgtgct ggcgcagctc ttcttcgccg
     1321 ggttggtggc gctcgcggtc ttcacctcgg accgctggcg cggaccgatc gagccgaccg
     1381 tccgtcgcgg cgcacggacc gacctcaggc tcggtcccac actcgtcgcg gtgaccgtcg
     1441 tccaactcgt gctcggcgcg tcgcagcgcc atttccagca gctcctgatg gcgcacctga
     1501 ccctcggctt gctcgtcgtc ctcccgctcg cgttggccgt cggcagccga gcctggggtc
     1561 tcgcgaccgg gcgggacggg ttgctgcggc ggctgggtcg cctgctggtg gccgtcgtcc
     1621 tggtccaggt ggtcctcggt ttcggcgccc tggtcgtgac gctggcgggg tcggacgggg
     1681 cgatcgggcc ggcctcgctc ctggtcttcc cgaccgccca ccagtggctc ggggcggtcc
     1741 tgttggccgt gacgacggcg ctcgtttgct ggaattcgcg ctgtctcgac gagcccggag
     1801 cggggcgcac ggggtgaggt gccgcgggtt cctccacgga ccgccggacc gcacgatcga
     1861 ggtggaaaag ctggaagggc ggtcggttcc accgtatacg gataccggtt gtcgcgtggc
     1921 gcatcgtcga ccccccgagc gatgggtccc cgccagggca ccggtgcgcg ggtcgggcaa
     1981 acggattgac agacggtgac ttaggcgcaa ccagcggtgg tttgccccga gatgccgccc
     2041 cgggagtcga gttgttgacc acgtcgaccc gccgtgacct cgtcaggcgt catcgcgacg
     2101 ttttcgtcgc gctgctcgcg ctcgcgatct gggcgcccgg cgcctcggcc gacgtcgtga
     2161 tccacgagat catgtaccac ccgctgtcca acgccgactc ggacgaattc gtcgagatct
     2221 tcaacaccgg cggctcgccc gtcgatatct ccaattggtg cttcgacggc gtcgatttct
     2281 gcttccccgc cgggggcacg acgatcggcg cgggagattt tctcgtcatc gcgaacgatg
     2341 ccgtcgggtt ccagacgacc tacggctttc cgccggatca cgtcttcact cccgcgacgg
     2401 cgagcgtgct cgacgacgcc ggcgagcggc tggcgctcat cgaccagggc ttcgtcatcg
     2461 tcgacgagct gctgttcacc gacgagccgc cgtggccggt cacaccggac ggtctcggcc
     2521 cgtcgctcga ggtgatcgac gccacgctcg acaactcgac cccgcgcaac tggcgggcgt
     2581 cgctcgccgc cggcggtacg ccgggcgcgg cgaacagcgt cgccgccacg ggtctgccgc
     2641 cgtggatcga ccagatccag cacacccagg acgtgctccc gctcgatccg gtcgtcgtca
     2701 cggcgcgggt cgttgccgcg acgaccgtcg acctgacgta caagatcgac ttcggcggcg
     2761 aagtcacgct gcagatgctc gacaacggct cgagcggcga cggcggggcg ggcgacgggg
     2821 tctacggcgc ggcgatcccc ggccaaccga tcgggacact cgtccgttac cgcatcaccg
     2881 ccaccggacc caccgggacc atccgttacc cccgggccga cgacaccgtg acctacgacg
     2941 gtaccgccgt cgtcgatccg gcgctgagct cggagctgcc gatcctccat tggttcatcg
     3001 atccgatcga ctaccaggag gcgctcgatc accggttgac cgacgaaacc gagcccgcgg
     3061 tgctgttcta cgacggcacg ctgtacgacg cggtcgagac gcggattcgc ggggcgtcgg
     3121 cacgccagtg gcccaaaccg ccgtggaagt tcttcacgcc ccagggtcac aacttcgccg
     3181 cacccggctt gatcgtcaac gacgtcgaca cgttcaacat ccagagcggc tacgccgaca
     3241 agagctacac ccgcgaggtg ctggcgtggg agacggtcgg tgaggcgggg ctcccggccg
     3301 gccgggtctt cccgatccgg gtcgagcaga acggtcagtt cttcggtttg ttcaccttcc
     3361 tcgaagcccc agattccgat ttcgtcgtgc gcaatcggct cgacgtgcgt ggcgcccgct
     3421 acaaggcctt cgagaatcta cggatgcaca tgacgatcga cgagatcgtt ccgctctacg
     3481 agaagaagtc gcggctggag gaggatcact cggatctgtg gcagctgatc acgcaggtca
     3541 accagccggc gacgcagcag atccacgatt ggctggccga caacatcgat atcccgacgg
     3601 tggtcaacta catcgccgcg atgtcgatcg tccacaacaa cgaccacttg aacaagaact
     3661 actttctcta tcgcgacacc
//
LOCUS       DASPGN010000427         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_546367, whole genome shotgun sequence.
ACCESSION   DASPGN010000427 DASPGN010000000
VERSION     DASPGN010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546367"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1864
                     /locus_tag="VD788_12410"
     CDS             <1..1864
                     /locus_tag="VD788_12410"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXV77111.1"
                     /translation="VDLNKPMTIDIGNVTLERALDILMLQTKNFYKVLDEYTLLVAPD
                     TRQKRQEYEDQVIRTFFLSNADTKTVVTLLRSLLQSRQIAENAELNSVTIKDTPSKVA
                     IAERIINANDKSRGEVLVDVELLEINRTLARTIGLDLSSKILSLQFGNGEQSVPLNNL
                     DVLKQSGNWTIGVIPTVTLDFLKTDSSTKFLAKPQLRVSEGERAEILIGDRVPIPTTS
                     FNTSQTVGGNIVPITSFTYQNVGITVQIEPRVHHNKEITLRVSVEASQVTGSVATGVG
                     GQEQPIIGTRQIQTVIRLRDNETNLLAGLIQTADQDALSTVVGLGDIPGLRRLFGRSG
                     LQKSSTDIIMALTPRIIRVPDITEDDLATLWVGTEENMQLRGTARDALGQGPFSGEPV
                     DTSLGEEAAVAGGGGGAVSRISSSPEVERDRAQAAAEAERRRQEDELEVDAPVEDEGG
                     PGGPVVPPQDQPEEPPAEEPEEPEDEEQPPSGPASVRLVPSKPVYQVGERVVVQVVID
                     SASNVGSTPFHLHYNPATLEFIQPGTEGPFMRSDGANTVFLAADTGGGGEIVVGLSRM
                     GAATGVDGSGTLAVFEFTAVAAGDCGFAFSGASVRDPQAGGLNAAFNAASVRVE"
     gene            1909..2370
                     /locus_tag="VD788_12415"
     CDS             1909..2370
                     /locus_tag="VD788_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77112.1"
                     /translation="MTLVELVAVSAILIIMASLALPVANTMVKRQKELELRRALREMR
                     EAIDRFQFDAQLYPGIKTKYLSGTNEEGYPEELAWLVEGVDAGATASADEVRLKYLRR
                     IPLDPITGKAEWGTRSSRDRPDSLFSDGINIFDVHSMSDKQGLNGVPYSEW"
     gene            2381..2791
                     /locus_tag="VD788_12420"
     CDS             2381..2791
                     /locus_tag="VD788_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXV77113.1"
                     /translation="MRNRAVTRRPSPGFTLIELLVVVAIIGILATVAVGQYQRNIVKA
                     KEAVLRENLFTMRTQINNYFADKGRYPMDLQELVDDRYLREVPFDPITRSNDSWIVEY
                     VELTDEDISTEPGIEDVRSGAEGTSLDGTAYGEW"
     gene            2791..>3032
                     /locus_tag="VD788_12425"
     CDS             2791..>3032
                     /locus_tag="VD788_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77114.1"
                     /translation="MGRRTTPARQAGYLMAALAAAVAVMVIFSTIAFQAWIDVLQRDN
                     EAEMMFRAKDIARAIQRYRRDHGGAPPVKLEQLLEPG"
BASE COUNT          557 a          975 c         1056 g          444 t
ORIGIN      
        1 agtcgatctg aacaagccga tgacgatcga catcggcaac gtcacgctcg agcgcgcgct
       61 cgacatcctg atgctgcaaa ccaagaactt ctacaaggtg ctcgacgagt acacgttgtt
      121 ggtcgcaccc gacacccggc agaagcgcca ggagtacgag gatcaggtga tccgcacctt
      181 cttcctcagc aacgccgaca ccaagaccgt cgtcacgctg ctgcggtcgc tgctgcaatc
      241 gcggcagatc gccgagaacg ccgagctcaa ctcggtgacg atcaaggaca cgccgtccaa
      301 ggtggcgatt gccgagcgga tcatcaacgc caacgacaag tcgaggggcg aggtgctggt
      361 cgacgtcgag ctgctcgaga tcaatcgcac gctggcgcgc acgatcggcc tcgacctgtc
      421 gtcgaagatc ctctcactgc agttcggcaa cggcgagcag agcgtgccgc tcaacaacct
      481 cgacgtgctc aagcagagcg gcaactggac gatcggcgtg atcccgaccg tcacgctcga
      541 tttcttgaag accgactcgt cgaccaagtt cctggccaag ccgcagctgc gggtgtcgga
      601 aggcgagcgc gccgagatcc tgatcggcga tcgcgtgccg atcccgacga cgtcgttcaa
      661 cacgtcgcag acggtcggcg ggaacatcgt gccgatcacc tcgttcacct accagaacgt
      721 cgggatcacg gtgcagatcg agccgcgcgt ccaccacaac aaggagatca cgctgcgcgt
      781 gtcggtcgag gcgagtcagg tgacggggtc ggtcgcgacc ggcgtcggcg ggcaggagca
      841 gccgatcatc ggtacgcggc agattcagac cgtcatccgc ctgcgcgaca acgagaccaa
      901 tctgctcgcc ggcttgatcc agaccgcgga ccaggacgcg ctgtcgaccg tcgtcggctt
      961 gggcgacatt cccggtctca ggcggctgtt cggacgcagc gggctgcaga agtcctcgac
     1021 cgacatcatc atggcgctga ccccgcgcat catccgcgtg cccgacatca ccgaggacga
     1081 cctggcgacg ctgtgggtcg ggacggaaga gaacatgcag ctccgcggca cggcgaggga
     1141 cgcgctcggt cagggaccgt tcagcgggga gccggtcgat accagcctgg gcgaggaggc
     1201 ggcggtcgcc ggcggaggcg gcggtgcggt gtcgcgcatc tcctcgtcgc ccgaagtgga
     1261 gcgggaccgg gcgcaggcgg cggccgaggc cgagcgacga cggcaggagg acgagctcga
     1321 ggtcgacgcg ccggtcgagg acgagggcgg cccgggtggc ccggtcgtcc cgccgcagga
     1381 ccagcccgag gaaccgcccg cggaggagcc cgaggagccc gaggacgagg agcagccgcc
     1441 gagcgggccg gccagcgtcc ggctggttcc gtccaagccg gtctaccagg tcggcgaacg
     1501 ggtcgtcgtc caggtcgtga tcgacagcgc ctccaacgtc ggctcgacgc cgtttcacct
     1561 gcattacaac ccggccacgc tcgagttcat ccagcccggg acggagggcc cgttcatgag
     1621 gtcggacggg gccaacaccg tctttctcgc agcggatacc ggcggtggtg gggagatcgt
     1681 cgtcgggctg tcgcggatgg gtgccgcgac cggtgtcgac ggctcgggaa cgctcgcggt
     1741 cttcgagttc accgcggtcg cggccgggga ctgtggcttc gccttctccg gcgcgagcgt
     1801 ccgggatccg caggccggcg gcttgaacgc ggcgttcaat gccgcctcgg tgcgggtcga
     1861 atgacagggc gcggtcgatc gcggtcgggg cgcgcggggg agcgcggagt gacgctcgtc
     1921 gagctcgtcg cggtctcggc gatcctgatc atcatggcca gtctggcgct acccgtcgcc
     1981 aacacgatgg tcaagcgcca gaaggagctc gagctgcgcc gggcgctgcg cgagatgcgc
     2041 gaggcgatcg atcggttcca attcgacgcg cagctctatc cggggatcaa gaccaagtac
     2101 ttgagcggga cgaacgagga gggctatccc gaggagctgg catggttggt cgagggagtc
     2161 gacgccggag cgaccgcctc cgccgacgag gtgcggctca agtacttgcg tcgcattccg
     2221 ctcgacccga tcaccggcaa ggcggagtgg ggcacgcgct ccagccgcga tcgaccggac
     2281 tcgctgttca gcgacgggat caacatcttc gacgtccact cgatgagcga caagcaggga
     2341 ctgaacggcg tcccgtactc ggaatggtag aggtgacggc atgcgcaacc gagcggtgac
     2401 acgacgcccg agcccgggct tcacgctgat cgagctgctc gtcgtcgtgg cgatcatcgg
     2461 cattctggcc acggtcgcgg tcggtcagta ccagcgcaac atcgtcaagg ccaaggaggc
     2521 ggtgctgcgc gagaacctgt tcacgatgcg gacccagatc aacaactact tcgccgacaa
     2581 gggtcgctat ccgatggacc tgcaggagct ggtcgacgac cgctacctgc gcgaggtgcc
     2641 gttcgacccg atcacgcgca gcaacgacag ctggatcgtc gagtacgtcg agctgaccga
     2701 cgaggacatc tcgaccgaac ccggcatcga agacgtccgt agcggggcag aaggcacctc
     2761 gttggacgga acggcctacg gagagtggtg atgggccggc gaacgacccc tgcgcgccag
     2821 gccgggtacc tgatggccgc gctggccgcc gcggtcgcgg tgatggtgat cttctcgacg
     2881 atcgccttcc aggcctggat cgacgtgctg caacgcgaca acgaagcgga gatgatgttt
     2941 cgcgccaagg acatcgcccg cgcgatccaa cgctaccgga gggatcacgg cggagcgccg
     3001 ccggtcaagc tcgagcagct gctcgagccc gg
//
LOCUS       DASPGN010000428         3459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_195055, whole genome shotgun sequence.
ACCESSION   DASPGN010000428 DASPGN010000000
VERSION     DASPGN010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195055"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1110)
                     /locus_tag="VD788_12430"
     CDS             complement(70..1110)
                     /locus_tag="VD788_12430"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77115.1"
                     /translation="MEIERLARGVVAVAVAVAVTVGAAAAAAGGDEQTGVDPAVSCHD
                     VALDGSTAFLAQTAGVSLWDLSDPTRPRATHRLPLPATVQRVVVRHPHLYLAAGSHGM
                     YIARLIEDGAPELISRHDTPGSAGDVALTGNYALLADDRYGVRVIDVSDPERPLQKAQ
                     LSTRDRVRSMSLAGGVLAIAEGSAGVRLIDIERPERPRELHLLRDAGDARDVAFVGDR
                     LLVAADRAGLLVYAFDREGKPSRVGQATVERGAKFVAGDRGLAVVSDGTPSLHLVGFD
                     DDGRPALIDRLRLHRSAPAGRVHLSGQLAYAALDRAGIAIVNLADPLAPDVLLPRERR
                     LEIIWPEARGDR"
     gene            complement(1100..2857)
                     /gene="aceK"
                     /locus_tag="VD788_12435"
     CDS             complement(1100..2857)
                     /gene="aceK"
                     /locus_tag="VD788_12435"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HXV77116.1"
                     /translation="MRETDARADERLVVRGADLIHRAFEALELSLGEVTSRARRQFER
                     RAWRELQASSERRLDLYSETLDRLEADVRRMLSGRVEQQELWRRMRQAYAGKLVDEQN
                     YELAQTFFNSVTRRIFSTVGVNPEIEFVGRRSALSDLSSRPGIYRRFDAREPLDELLV
                     ELLASYRFAARWCDLARDATRVARQVERRLGRGSRAARFDTIEMLRSVFFRNKGAYLI
                     GRIRRELHVIPFVVALTHGPDGIEVDAALLEQHEVSIVFSFTRSYFLVETDCPRQLIV
                     FLKSIMPLKPIADLYTSLGFNKHGKTELYRSLRRHLDKYDDRFEIAPGDVGMVMLVFT
                     LAGFDMVFKVIRDRFAPPKQTSHAAVMERYRLVFKHDRVGRLIDAHEFEHLTFARQLF
                     SERLLDELLRHAGQTVEPRGDEVVIHHLYAERKVTPLNIYLRRADRAAAERAVIDYGN
                     AIKELAAANIFPGDFLLKNFGVTRHGRVVFYDYDELCLLETCRFRALPPPRVPEDEWA
                     AEPWFGVRDEDVFPEEFRRFLGLGDELQQVFGRHHGDLFTVGFWQALQRRHEHGELVD
                     VYPYPAKRRLTRAGDGNGD"
     gene            complement(2864..>3459)
                     /locus_tag="VD788_12440"
     CDS             complement(2864..>3459)
                     /locus_tag="VD788_12440"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HXV77117.1"
                     /translation="SHTNPLWLHPEDARRIGVASGELVRVNTRIGHFVARAWVTEGIH
                     PGVIACSHHVGRWRLHREIGGHKIASAVVDIERAGRTFRFRRKAGIEPWDSVDPDTRR
                     VWWTDAGVNQNLTFPVQPDPVSGMHCWHQLVRPERALADDRQGDVFVDLDLAEGVYRE
                     WLARTTPAPGPGGLRRPLWLKRPLDPGPPAYRFPDGE"
BASE COUNT          550 a         1205 c         1211 g          493 t
ORIGIN      
        1 gtcccggcgt agccgcttgc ccgccgattc gttggccgcg ggcggcggcg ccgcgcactg
       61 accgcggtct cagcggtcgc cgcgggcttc cggccagatg atctcgagcc gccgctcccg
      121 cggcagcagc acgtcgggag ccagcggatc ggccaggttg acgatcgcga tgcccgcgcg
      181 atcgagcgcc gcgtacgcca gttgaccgct gagatggacg cgcccggccg gcgccgagcg
      241 gtggagccgc agccggtcga tcagcgccgg ccgcccgtcg tcgtcgaatc cgacgaggtg
      301 gagcgacggc gtcccgtcgc tgacgacggc cagaccgcga tcgccggcga cgaacttggc
      361 gccgcgctcg accgtcgcct ggccgacgcg ggacggcttg ccctcgcgat cgaaggcgta
      421 gaccaacagg cccgcccgat cggcggccac cagcaaccgg tcgccgacga acgcgacgtc
      481 ccgcgcgtcg cccgcgtccc gcagcagatg cagctcgcgc ggccgttcgg ggcgctcgat
      541 gtcgatcagc cgcacaccgg cgctgccctc ggcgatcgcc agcacgccgc cggcgagtga
      601 catcgaccgc acgcggtccc gcgtcgacaa ctgcgccttc tgcaacggac gctcgggatc
      661 cgacacgtcg atcacgcgta cgccgtagcg gtcgtcggcc agcaacgcgt agttcccggt
      721 caacgcgacg tcgccggccg agcccggcgt atcgtgacgc gagatcagct ccggcgctcc
      781 gtcttcgatc aggcgggcga tgtacatccc gtgcgatccc gccgccagat agagatgtgg
      841 gtgtcgcacg acgacccgct gaacggtcgc cggcagtggc aagcggtggg tcgcccgcgg
      901 ccgggtgggg tcggacagat cccacaacga cactcccgcg gtctgagcca gaaacgccgt
      961 cgagccgtcg agcgcgacgt cgtggcacga cacggccgga tcgacgccgg tttgctcgtc
     1021 gccgccggcg gccgcggcgg ccgccccgac ggtcacggca acggcaacgg caacggcaac
     1081 cacgccgcgc gccaggcgct caatctccat ttccgtctcc cgcgcgcgtc agcctgcgct
     1141 tggccgggta aggatagacg tcgaccagct cgccgtgctc gtgacgacgc tgcagcgcct
     1201 gccagaagcc gacggtgaac aggtcgccgt ggtgtcgtcc gaagacctgc tgcagctcgt
     1261 ctcccagccc gagaaagcgg cggaactcct ccgggaagac gtcctcgtca cgcacgccga
     1321 accacggctc cgccgcccac tcgtcctcgg gaacccgcgg cggcggcagc gcacggaaac
     1381 gacaggtctc gagcagacac agctcgtcgt agtcgtagaa gacgacgcga ccgtggcgcg
     1441 tgacgccgaa gttcttcaac aggaaatcgc ccggaaagat gttcgccgcg gccagctcct
     1501 tgatcgcgtt gccgtagtcg atcaccgcgc gctcggcagc ggcgcggtcc gcgcggcgca
     1561 ggtagatgtt gagcggggtc accttgcgct cggcgtagag gtggtggatc accacctcgt
     1621 cgccgcgcgg ctcgaccgtt tgtccggcgt ggcgcagcag ctcgtcgagc aaccgctcgg
     1681 agaacagctg gcgagcgaag gtcagatgct cgaattcgtg cgcgtcgatc agccgtccga
     1741 cgcgatcgtg cttgaacacc aggcggtaac gctccatcac cgcggcgtgg gaggtttgtt
     1801 tgggcggcgc gaaccggtcg cggatcacct tgaacaccat gtcgaacccg gccaaggtga
     1861 agaccaacat caccatgccg acgtcgccgg gcgcgatctc gaaacggtcg tcgtacttgt
     1921 cgaggtggcg ccgcaggctg cggtacagct ccgtcttgcc gtgcttgttg aagccgagcg
     1981 aggtgtacag gtccgcgatc ggcttgagcg gcatgatcga cttgagaaag acgatgagct
     2041 gccgcgggca gtcggtctcc accaggaagt acgagcgggt gaagctgaac acgatgctga
     2101 cttcgtgctg ctcgagcagc gccgcgtcga cctcgatccc gtcgggcccg tgggtcagcg
     2161 cgacgacgaa cgggatgacg tgtaactcgc ggcggatgcg gccgatcagg taggcgccct
     2221 tgttgcgaaa gaacaccgag cggagcatct cgatcgtgtc gaagcgcgcc gcgcgggagc
     2281 cgcgcccgag tcgccgctcg acttgacgtg cgacccgcgt cgcatcgcgc gcgagatcgc
     2341 accagcgcgc cgcgaaacgg tagctggcca gcagctcgac caacagctcg tcgagcggct
     2401 cgcgcgcgtc gaagcgacgg tagatcccgg gtcgcgagct caggtcggac aacgccgagc
     2461 ggcgaccgac gaactcgatc tcggggttga cgccgaccgt cgagaagatg cggcgtgtga
     2521 cggagttgaa gaacgtctgc gccagctcgt agttctgctc gtcgaccagc ttgccggcgt
     2581 aggcctgccg catccggcgc cacagttcct gctgctcgac ccgtccgctc agcattcggc
     2641 ggacatctgc ctcgagccga tcgagcgttt ccgagtagag gtcgagccgt cgctcggagc
     2701 tggcttgcag ctcgcgccag gcacgacgtt cgaactggcg ccgggcacgc gaagtgacct
     2761 cgcccaacga tagctcgagc gcttcgaacg cccgatggat caggtcggcg ccgcggacga
     2821 cgaggcgctc gtcggctctc gcgtcggtct cgcgcacgcc aagctactcc ccgtcgggga
     2881 agcggtaggc cggggggccg gggtcgagcg gccgcttgag ccacaacgga cggcgcagcc
     2941 cgccgggccc cggggcgggc gtggtccgcg ccagccactc gcggtagacg ccctcggcca
     3001 ggtcgaggtc gacgaagacg tcgccctggc ggtcgtcggc gagggcgcgc tcgggtcgca
     3061 cgagctgatg ccagcagtgc atgccgctca ccgggtcggg ctggaccggg aaggtcaggt
     3121 tctgattgac gcccgcatcg gtccaccaca cgcggcgcgt gtcggggtcg acgctgtccc
     3181 acggctcgat cccggccttg cgccggaaac ggaaggtccg gcccgcgcgt tcgatgtcga
     3241 ccacggcgct cgcgatcttg tggccgccga tctctcgatg cagacgccag cggccgacgt
     3301 ggtgcgagca ggcgatcacg cccgggtgga tcccttcggt gacccacgcc cgggcgacga
     3361 agtgaccgat gcgcgtgttg acacgcacca gctcgccgct ggcgacgccg atccgccgcg
     3421 cgtcctccgg gtgcagccac aacgggttgg tgtgcgaga
//
LOCUS       DASPGN010000429         4350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_781486, whole genome shotgun sequence.
ACCESSION   DASPGN010000429 DASPGN010000000
VERSION     DASPGN010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781486"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1327)
                     /locus_tag="VD788_12445"
     CDS             complement(<1..1327)
                     /locus_tag="VD788_12445"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXV77118.1"
                     /translation="MSKTYDAIVIGGGHNGLTCAAYLARAGRDVLVLERRHVLGGAAV
                     SEELHPGFTFSVCSYVVSLFRPRIIRELGLARHGLEIIPLDCSFLPLPDGDSLARWGD
                     AARTRREIARFSRRDADLYPEFGRAMGKLARFARHVINDPAPDPTSLDPRELAGLLRL
                     RRAMREFDGDLRYLNFKLLTMSAVDFLDEWFESEVLKSPMSVSGIIGTFLGVRSPGTA
                     YVLLHHYMGEIDGAFRAWGFAKGGTGRVSLAIADAARAFGAEIRTGAGVARVRVRSGR
                     ATGVVLDNGDELAARQVVSAVDPHRTFLRLVGEEHLDGEFVEQIRRYKLRGSSGKVNL
                     AVDRLPEFACRPNGGGAAHLRGDIAIAPGVEYLERAYDEAKYGDFSRRPYINVVIPSV
                     VDPSVAPPGQHVMSVFVQYAPYAIKEGPEHWPERREAFGDAVIDTLSDYC"
     gene            complement(1324..2514)
                     /locus_tag="VD788_12450"
     CDS             complement(1324..2514)
                     /locus_tag="VD788_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HXV77119.1"
                     /translation="MPVPTPFHPRTSELCTSLLWKEWAGHYAVCSYGTCHEREYFALR
                     HAAGLIDVSPLHKYEIAGPGAGAFLSFLTTRDLSKLRVGRMTYLCWCDDDGKVLDDGT
                     AGRLDKQHYRLTSNQPALSWLERNAAGFDVAIEDSSERLGVVALQGPTSRAILAAAAG
                     PAVESLRFFAVSRGTIDGIPVWISRTGYTGDLGYEVWVERDGALGVWDALVAAGRPYR
                     IRPAGLNAMDVTRVEAGFVLNGVDYFSANTCLIPARRSSPYEIGLGWTVTLDREPFIG
                     QAALRAECARPADWRLVGLEYDWDEFEALHDEFGLPPHVPSAAWRSAVPVYDGERRQV
                     GQATSGAWSPILKKNLALATLRPAAASTGSHLRVETTVEYERRTVGATVVGTPFFDPP
                     RKRA"
     gene            2722..3120
                     /locus_tag="VD788_12455"
     CDS             2722..3120
                     /locus_tag="VD788_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77120.1"
                     /translation="MRIFERLAAVTVIVALAALPALASGEDMKKKEDPKAAAAQAGEA
                     KALEAKAGDAKAAGTDAVAGAQTKKKNCPTDGSFNTMEDMFGAEEVAAMLAAHQQRLA
                     QDAQTVQWQAVAEPEDGKDEKKKKSDKSKD"
     gene            3161..3430
                     /locus_tag="VD788_12460"
     CDS             3161..3430
                     /locus_tag="VD788_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXV77121.1"
                     /translation="MPQVKFTQNLERHLSAPAIDVAGRTVREALDAVFHGNPMLRSYV
                     LDDQGRLRRHVVVFVDGQMVEDRDGLTDAVESSSELFVMQALSGG"
     gene            3435..>4350
                     /locus_tag="VD788_12465"
     CDS             3435..>4350
                     /locus_tag="VD788_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77122.1"
                     /translation="MEHGDRVRVATRKGLFTLERAGSGRWQIGRMSFIGDPVTIVLDD
                     RRDGSLYAALNLGHFGVKLRRSRDGGETWDELVHPAFPEQENGPSVHQIWSLEPGGDD
                     RPGRLWAGTIPAGLWRSENHGDSWRLVEPLWNRPERANWFGGGYDEAGIHSICVDPRD
                     SNALAVGVSCGGVWISNDSGETWECRTRGMYAAYMPPERREDPDIQDPHRVSQCAGAP
                     DVLWVQHHNGVFRSTDRARQWRELADIEPSTFGFAVAAHPTDPRTAWFVPAVKDECRV
                     PVGGRVVVTRTRDGGESFEVLDRGLPQQH"
BASE COUNT          668 a         1507 c         1586 g          589 t
ORIGIN      
        1 ggcagtagtc ggacagcgtg tcgatcaccg cgtccccgaa ggcctcccgc cgctcgggcc
       61 agtgctcggg accctccttg atcgcgtagg gcgcgtactg cacgaagacg gacatcacgt
      121 gctggcccgg cggggcgacg ctcgggtcca ccaccgaggg gatcacgacg ttgatgtacg
      181 gccgccgcga gaagtcgccg tacttcgcct cgtcgtaggc gcgctccagg tactcgacgc
      241 ccggcgcgat cgcgatgtcg ccgcgcaggt gcgcggcgcc gccgccgttg ggccggcacg
      301 cgaactccgg cagccggtcg acggccaggt tgaccttgcc cgagctgccg cgcagcttgt
      361 agcgccggat ctgctcgacg aactcgccgt cgagatgctc ctcgccgacg aggcgcagga
      421 aggtgcggtg cgggtcgacg gcggagacga cctgacgcgc cgccagctcg tcgccgttgt
      481 cgagcaccac gccggtggcg cggcccgacc gcacgcgcac gcgcgcgacg ccggcaccgg
      541 tgcggatctc ggcgccgaac gctcgcgcgg cgtcggcgat cgccaggctg acgcgtcccg
      601 tcccgccctt ggcgaagccc caggcgcgga aggcgccgtc gatctcgccc atgtagtggt
      661 gcagcaggac gtaggccgtg cccggcgagc gcacgccgag gaaggtaccg atgatgccgc
      721 tgaccgacat cggcgatttg agcacctcgc tctcgaacca ctcgtcgagg aagtcgaccg
      781 cgctcatcgt cagcagcttg aagttcaggt agcgcaggtc gccgtcgaac tcgcgcatcg
      841 cccgccgcag ccggagcagc ccggccagct cgcgcgggtc gagcgacgtc gggtcgggcg
      901 ccgggtcgtt gatcacgtga cgcgcgaagc gggccagctt gcccatcgcc cggccgaact
      961 cgggatagag gtcggcgtcg cgccggctga agcgcgcgat ctcgcgtcgc gtcctcgcgg
     1021 cgtcgcccca gcgcgccagc gagtcgccgt ccggcagcgg caggaacgag cagtcgagcg
     1081 ggatgatctc gaggccgtgc cgcgcgagac cgagctcgcg gatgattcgt ggccggaaca
     1141 ggctgacgac gtaggagcag accgagaagg tgaagcccgg gtgcagctct tcgctgaccg
     1201 ccgcgccacc cagcacgtga cgtcgctcga gcaccagcac gtcgcgaccg gcccgggcca
     1261 ggtaggccgc gcaggtcagc ccgttgtgcc ccccgccgat gacgatcgcg tcgtaggtct
     1321 tgctcacgcg cgtttcctcg gcgggtcgaa gaacggcgta ccgacgaccg tggcgcccac
     1381 cgtgcgccgc tcgtactcga ccgtggtctc gacgcgcagg tggctgcccg tcgacgcggc
     1441 ggccggtctg agcgtggcga gcgccaggtt cttcttgagg atcggcgacc acgcgccgct
     1501 cgtcgcctgg ccgacctggc gccgctcgcc gtcgtagacc ggcacggcgc tgcgccaggc
     1561 ggcggacggc acgtgcggcg gcagcccgaa ctcgtcgtgc aacgcttcga actcgtccca
     1621 gtcgtactcg aggccgacca gccgccagtc ggccggtcgc gcgcactcgg cgcgcagcgc
     1681 ggcctgaccg atgaacggct cgcggtcgag cgtgaccgtc cagccgagcc cgatctcgta
     1741 gggcgacgag cgtcgcgccg ggatcaggca ggtgttggcg ctgaagtaat cgaccccgtt
     1801 cagcacgaac cccgcctcga cccgcgtcac gtccatcgcg ttcaggccgg cgggcctgat
     1861 gcggtagggg cggccggcgg ccaccagcgc gtcccacacg ccgagcgccc cgtcgcgctc
     1921 gacccacact tcgtagccga gatcgcccgt gtagccggtg cgcgagatcc agaccgggat
     1981 cccgtcgatc gtcccgcgac tcacggcgaa gaagcgcaac gactcgacgg ccggcccggc
     2041 cgccgcggcg aggatcgccc gcgaggtcgg cccctgcagc gcgacgaccc cgaggcgctc
     2101 gctcgaatcc tcgatcgcga cgtcgaagcc ggcggcattg cgctcgagcc aggagagtgc
     2161 gggctgattc gaggtcaacc ggtagtgctg cttgtcgagc cgtccggccg tgccgtcgtc
     2221 gagcaccttg ccgtcgtcgt cgcaccagca caggtaggtc atccggccga cgcgcagctt
     2281 gctcaggtcg cgcgtggtga gaaacgacag gaacgcgccg gcacccgggc ccgcgatctc
     2341 gtacttgtgg agcggcgaga cgtcgatcag accggcggcg tgccgcagcg cgaaatactc
     2401 gcgctcgtgg caggtgccgt aggagcagac cgcgtagtgg ccggcccact ccttccacag
     2461 caggctggtg cagagctcgg aggttcgcgg atggaacggg gtcggtaccg gcatggcgtc
     2521 cgtcatcgat cgggcggaag cgagcgccga ttctgacaca caaccgggcg ccggcggtag
     2581 gccgcggggg cggtgtgcgg tgcgccgggc ggtgccgatt tccgcggtgt tccggcttgg
     2641 atgtggacga agccgcccgc tcggggtata cagaggtggt tcaggctccc gcaccacacg
     2701 cgggcccccg gaggatgaac cgtgcgcatc ttcgagcgtc tcgccgccgt gaccgtcatc
     2761 gtcgccctcg ccgccctgcc ggcgctcgcc tcgggcgagg acatgaagaa gaaggaagac
     2821 ccgaaggccg ccgcggcgca ggccggagag gccaaagccc tagaggccaa ggccggcgac
     2881 gccaaggccg cgggcaccga cgccgtcgcg ggcgcgcaga cgaagaagaa gaactgcccg
     2941 accgacggct cgttcaacac gatggaagac atgttcggcg ccgaggaggt cgcggcgatg
     3001 ctggcggccc accagcagcg gctggcgcag gacgcgcaga ccgtccagtg gcaggccgtc
     3061 gccgaaccgg aagacggcaa agacgagaaa aagaagaagt cggacaagag caaagactga
     3121 gccgtcccgc cgccggtcgg accacccgcc gggggtcgtc gtgccgcagg tcaagttcac
     3181 gcagaatctc gagcgtcatc tgtccgcgcc ggcgatcgac gtcgcgggcc gcacggtccg
     3241 cgaggcgctc gatgcggtct tccacgggaa tccgatgtta cgcagctacg tgctggatga
     3301 ccagggacgg ctgcgccggc acgtggtcgt gttcgtcgac ggacagatgg tcgaggaccg
     3361 cgacggtctg accgatgcgg tcgaatcgtc gagcgagctg ttcgtgatgc aggcgctgtc
     3421 ggggggctga ccagatggaa cacggagatc gcgttcgcgt cgcgacgcgc aagggtctgt
     3481 tcacgctcga gcgcgcgggg tccgggcgct ggcagatcgg ccgcatgtcg ttcatcggcg
     3541 atccggtcac gatcgtgctc gacgaccgcc gcgacggctc gctctacgcg gcgctgaacc
     3601 tcggccactt cggcgtcaag ctgcgccggt cgcgcgatgg cggcgagacg tgggacgagc
     3661 tggtccaccc ggcgttcccc gagcaggaga acggaccgtc cgtgcatcag atctggtcgc
     3721 tcgagccggg aggggacgat cggccgggcc gcctgtgggc cggcacgatc cccgccgggc
     3781 tctggcgctc ggagaaccac ggcgacagct ggcggctggt cgagccgttg tggaaccgcc
     3841 cggagcgcgc caactggttc ggcggcggct acgacgaggc gggcatccac tcgatctgcg
     3901 tcgacccgcg cgactcgaac gcgctggccg tcggcgtctc gtgcggcggg gtgtggatca
     3961 gcaacgattc gggcgagacg tgggagtgcc ggacgcgcgg gatgtatgcg gcctacatgc
     4021 cgcccgagcg ccgcgaggat cccgacatcc aggatccgca tcgcgtgtcg cagtgcgccg
     4081 gcgcacccga cgtgctgtgg gtgcagcacc acaacggtgt cttccgcagc acggatcggg
     4141 cgcgccagtg gcgcgagctg gccgacatcg agccttcgac gttcggcttc gcggtcgcgg
     4201 cccacccgac cgacccgcgg accgcctggt tcgtgccggc ggtcaaggac gagtgccgcg
     4261 tacctgtcgg cggccgggtc gtcgtcaccc gcacgcgtga cggcggcgag agcttcgagg
     4321 tgctcgaccg cggtctgccg cagcaacacg
//
LOCUS       DASPGN010000430         9885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1365288, whole genome shotgun sequence.
ACCESSION   DASPGN010000430 DASPGN010000000
VERSION     DASPGN010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9885
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365288"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="VD788_12470"
     CDS             <1..1395
                     /locus_tag="VD788_12470"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXV77123.1"
                     /translation="GETTPVVWDRAEYEYLAAVRWEPDAPLTILVQNRRQTEERLLTV
                     DPASGRTSTLLIESDASWLNLHDAMPFWLPGGDRFLWVSERDGAPRLELRARDGTRLR
                     ALDGGQSGFRRFEAYLERADAVVVRAGTDPTRASLHSLSLTGGARSKPLGDGDGVESV
                     VSSGSRELIVYRSAGENRSERYRLAGAGGPLPLELRSAAELPPSMPRLEFVEVGDGPS
                     FHAVLVRPRTYEAGQRYPVLLHVYGGPGSQMVQRDAGRYLLDQWFADHGYVVVSIDGR
                     GTPGRGRDWERAIKGNLIERPLQDQVAALRALGARFPELDLERVGIWGWSFGGYFSAM
                     AVLMRPDVFHVGAAGAPVVSWEGYDTHYTERYMDLPQLNPSGYRDADALTYAGGLRRP
                     LMLVHGTADDNVYFVHSLKLADALLREGKPFEFLPLPGLTHMVSDRQVVRRMYHRMLA
                     LFDEHLARRDAGTE"
     gene            1446..1631
                     /locus_tag="VD788_12475"
     CDS             1446..1631
                     /locus_tag="VD788_12475"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HXV77124.1"
                     /translation="MGLYRSRTDRVLGGVCGGIAHWLGWSPNLVRVLYVVVSILSAGF
                     PGTLVYIVLWILMPQAD"
     gene            complement(1639..2640)
                     /locus_tag="VD788_12480"
     CDS             complement(1639..2640)
                     /locus_tag="VD788_12480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV77125.1"
                     /translation="MRFDGGVPAIRPAGAAVLVLTAALAALAAADGPQPAEVGSPSDD
                     GRIERLRRLHYTESHQVSMVLLPTSVTDRRGRNVIDLTKEDFRLLEDKVPQNIRYFSS
                     EAREPISVAFVLDVSGSMRQLGKMDHAKEAIRFFVDQLRYDDRFALICFADEQVDWVT
                     EFTADRQRFMRRLSVQEGYGKTALNDAIAAAPSLVDDGIKGRKAIVLITDGVDNSSRI
                     GIEQAVDIARRVSVPVYSIGFLSVAENMLPKDVAAMNLEVLRFVSAETGGKLFAVHDP
                     DELKEAVTAVDRELRHQYLLGYYPPRDKRPGGYREIQVSTERKRLTVRTRKGYYATP"
     gene            2883..3734
                     /locus_tag="VD788_12485"
     CDS             2883..3734
                     /locus_tag="VD788_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77126.1"
                     /translation="MTFLERLLSDDPLLSIEMRPPRADLSAEASMDSWLAMGSAVRRL
                     ATKDTALFFTDNAVGANEEENLHHLVTNLEDEVARLRICPFLTTKHTLEYCLWYADRA
                     VYDGHTALTVLGGDRHVGPPRCVPHSDELRRRIRQRHRGLALGAWANPYHDAAAQVGY
                     LADGRATADFFLSQVVSHHELGPVERFLERAEREGLALPGVFGVFYYRSANPRTFALL
                     SSFLRVPAEEITRDFEQRGKSADEICAETIRALRRLGVRHVYISNLKPDEAPKRLAAI
                     GAIVRAG"
     gene            complement(3983..5848)
                     /locus_tag="VD788_12490"
     CDS             complement(3983..5848)
                     /locus_tag="VD788_12490"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HXV77127.1"
                     /translation="MDRHAPTKPKVKPGQEPFRRLLEQGGVALFDGAMGTMFYSRGVF
                     INRAFEELNLTRPSLVREVHAEYLAAGADVLETNTYMANRFRLSAHGLSDRVEEINTR
                     AVEIAREAGSGAHWVAGAIGPLGVRIEPFGPISREEARAVFAEQARALAAAGVDLFVI
                     ETFSNLSELQQAIFAVREVSDLPLIAQMTVGKGGVTPEGVDAGEAAAKLVAAEADVVG
                     VNCSEALATLDALEAMRKAVDAPLSGQPNAGQPRTVQGRHLYLASPDYLVAWARRALR
                     VGARVLGGCCGTTPDHIRMLCQAVRETEPVTPPASVGRAIEIAPAAEPPSRKERSVLA
                     GALASSRFVVGAEMPLPIGWETSGIVAAARRLAAAGASFLALPEDSRSEARMPPYAMA
                     QLLAPVRGIEPVVYYACRERRLARIQSDLLGVWASGLANLLLVTGEPPHSPLDAGGSE
                     LDVDAIGAVNLASRLNHGEDIGGNPIGKPTGFHIGVRLDPTSYDREQEVRRLYWKVDA
                     GAEYALTSPVFDLDALQSLVADTREFKIPIIATVWPLRSAREAEFFEHEMVDVPVPKT
                     IVERMQRAERDGTEAREGVAIARELTVGVRDLVHGLQVYVPGGRLETALEVLEAL"
     gene            5962..6702
                     /locus_tag="VD788_12495"
     CDS             5962..6702
                     /locus_tag="VD788_12495"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77128.1"
                     /translation="MASDRSVRIAMWSGPRNISTAMMRSWGNRPDTFVCDEPLYAHYL
                     LSTGLSHPGADEVIARHETDWRRVVAWLTGPVPEGRTIFYQKQMTHHLLPEIDRGWLD
                     ELRHAFLIRDPGPMLASLASVYPHPTLDDTGLPQQVEIFERVRRTTGNLPPIVDAREV
                     LADPERMLRKLCAALGVEFTPRMLSWAPGPRATDGVWAKHWYDAVVKTTGFLPAAPRE
                     RPAPPGMDDLLRECREPFELLYRHRLRP"
     gene            6731..7639
                     /locus_tag="VD788_12500"
     CDS             6731..7639
                     /locus_tag="VD788_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXV77129.1"
                     /translation="MLQSFDERNRDLEINVNGRLVHRDEAGISPFDSAVQGGDAVWEG
                     LRLYNGRVFKLREHLTRLRGSARALGFESIPSDEAIIEEIRRTLVANRMFDGVHIRLT
                     LTRGVKITSGMDPRLNTRGPTLIVLAEHKPPVYAKTGLTLITSSVRRAPPDCLDPKIH
                     HCNLIQSILAKIEANAAGADDAVMLDPRGFVAETNATHLFLVERGTVATPRTVACPEG
                     VTRATVLELCAAERIPTRVGDLSLAEFYRADEVFCSGTMGELAAVVAIDGRRIGGGEP
                     GPLTLRLDAAYRCETATGGEPLVDRA"
     gene            7721..8278
                     /locus_tag="VD788_12505"
     CDS             7721..8278
                     /locus_tag="VD788_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV77130.1"
                     /translation="MEVLAIRAPGNHGAIDPLVARAKAGDFGAFEGLYNAHLHGVYAI
                     CMRMTANRSRAEELTQETFVRAWQRLAQFRGEGGFAGWLRRMAVNVVLGDSRATARRR
                     RRETVPERTPAFPGAAAAHEPARAIDLEHAIERLPTRARHVFVLHDIEGYRHEEIARR
                     LGVAVGTSKAQLHRARKLLREALRE"
     gene            8275..8931
                     /locus_tag="VD788_12510"
     CDS             8275..8931
                     /locus_tag="VD788_12510"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV77131.1"
                     /translation="MNCIDYELLLDDYVDGELDGARRAEVERHVATCAACRAALGELG
                     QLASAVGELPRGLAPERDLLPGIRRTALGRGGARWRLWAGLAASVALAATAVWIGLHY
                     AGRPGTPPDREPGRLSADRERAWSVAQVTRAEFELAEQEYEEATARLLRLLDARADSL
                     SPETRELVAENLRIIDQAIAELRTALDNDPNDARNGHALNSLYRHKVEFLWLMSRLSS
                     "
     gene            8957..9745
                     /locus_tag="VD788_12515"
     CDS             8957..9745
                     /locus_tag="VD788_12515"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXV77132.1"
                     /translation="MRRIAKTWTTLIVALGCVAAVAGERQVDQTIEAAADGTVGIELI
                     SGSVRFVGWSGDEIRITGTLGRDVEGLDVRKSDNSVRIEVELVERSGGMHEADADLEI
                     RVPADSRIEAESVSADLEIEGIRGEVSIESVNGEVQIRGEVTEAEVATVSSDVHVASD
                     GRLRDGRFETVSGSIEFEGALDPTGRFDFEAVSGDITLRIPAGTAADFDVETFSGTIV
                     NELGPEPRRTSEHLPSKSLAFSTGAGGARVSIESFSGTVRILGR"
BASE COUNT         1445 a         3448 c         3507 g         1485 t
ORIGIN      
        1 ggcgagacga cgccggtcgt ctgggatcgc gcggaatacg agtacctggc ggcggtgcgt
       61 tgggagccgg acgcgccgtt gacgatcctg gtacagaacc gccggcagac cgaggagcgc
      121 ctgctgaccg tcgacccggc gagcggtcgg acctcgacgc tgttgatcga gagcgacgcg
      181 agctggctca acctgcacga cgcgatgccg ttctggttgc ccggcggcga tcgcttcctc
      241 tgggtcagcg agcgcgacgg cgcgccgcgg ctcgagctgc gggcccgcga cggcacgcga
      301 ctccgcgcgc tggacggcgg gcagtcgggg ttccgccggt tcgaggccta tctcgagcgg
      361 gccgatgccg tcgtcgtgcg agccggcacg gacccgaccc gagcgagtct ccactcgctg
      421 tcgttgaccg gcggcgctcg gtcgaagccg ctcggcgacg gcgacggcgt cgagtcggtc
      481 gtgtcgagcg gctcgcgcga gttgatcgtc tatcgcagcg ccggcgaaaa ccggtcggag
      541 cggtaccggc tggccggcgc cggtggcccg ctcccgctcg agctgcggtc ggcggccgag
      601 ctgccgccgt cgatgccccg tctcgagttc gtcgaagtcg gtgacggtcc gagcttccac
      661 gccgtgctcg tgcgcccccg gacgtacgag gccgggcaac gctatccggt gctgctccac
      721 gtctacggcg gccccggctc gcaaatggtc cagcgcgacg ccggacgcta cctgctcgac
      781 cagtggttcg ccgaccacgg ctacgtcgtc gtctcgatcg acgggcgagg cacgcccggt
      841 cgcgggcgcg actgggaacg cgcgatcaag ggcaacctga tcgaacgccc gctgcaggac
      901 caggtggccg cgctgcgcgc gttgggcgcg cgcttcccgg agctcgacct cgaacgggtc
      961 ggcatctggg gctggtcgtt cggcgggtac ttcagcgcga tggccgtgct gatgcgtccg
     1021 gacgtcttcc acgtcggggc cgccggcgca ccggtggttt cctgggaggg ctacgacacg
     1081 cactacaccg aacggtacat ggatctcccg cagctcaacc cgtcgggcta tcgcgatgcc
     1141 gacgcgctga cctacgccgg cggcctgcgg cggccgttga tgctggtcca cggcacggcc
     1201 gacgacaacg tctacttcgt gcacagcctc aagctggccg atgcgctgct gcgcgagggc
     1261 aagccgttcg agttcctgcc gctgccgggg ctgacccaca tggtgtccga ccgtcaggtc
     1321 gtgcgacgga tgtatcatcg gatgctcgcg ctgttcgacg agcatctcgc gcggcgcgac
     1381 gccggcacgg aatgaacgag ctcgttgcgc gaggcgcggg cgagccggga gcaggaggcc
     1441 gaccgatggg gttgtaccgg tcgaggacgg atcgggtgct cggtggggtt tgcggcggga
     1501 tcgcccactg gttgggctgg agtcccaacc tggtccgcgt gctctacgtc gtggtctcga
     1561 tactgagcgc gggcttcccc ggaacgctgg tctacatcgt gctatggatc ctgatgccgc
     1621 aggccgactg accggccctc agggggtcgc gtagtagccc ttgcgtgtgc gtacggtcag
     1681 tcgcttgcgc tcggtcgaca cctggatttc gcggtagccc cccggtcgct tgtcccgcgg
     1741 cgggtagtag cccagcaggt actggtggcg cagctcgcga tcgaccgcgg tgacggcctc
     1801 cttcagttcg tccggatcgt ggacggcgaa caacttgccc ccggtctccg cgctgacgaa
     1861 ccgcagcact tcgaggttca tcgccgctac atccttgggc agcatgttct cggcgaccga
     1921 caggaacccg atcgaataga ccggcacgct cacgcgccgc gcgatgtcga ccgcctgctc
     1981 gatgccgatc cgactcgagt tgtcgacgcc gtcggtgatc agcacgatcg ccttacgccc
     2041 tttgattccg tcgtcgacga gcgacggcgc ggccgcgatc gcatcgttca acgccgtctt
     2101 gccgtagcct tcctggaccg acagccggcg catgaaccgc tgccggtcgg cggtgaactc
     2161 ggtgacccag tccacctgtt cgtcggcgaa gcagatcagg gcgaaccgat cgtcgtaacg
     2221 gagctggtcg acgaagaagc gaatcgcttc cttggcgtgg tccatcttcc ccagttgccg
     2281 catgctgccc gacacgtcca gcacgaaggc gaccgagatc ggttcgcggg cctccgacga
     2341 gaagtagcgg atattctgcg gcaccttgtc ctcgagcagc cgaaagtctt ccttggtcag
     2401 gtcgatcacg ttgcgaccgc ggcggtcggt cacgctggtc ggcagcagca ccatcgacac
     2461 ctgatggctc tcggtgtagt gcaggcggcg caggcgctcg atgcggccgt cgtccgacgg
     2521 cgacccgacc tccgccggct gcggtccgtc tgccgcggcg agcgcggcca gtgccgccgt
     2581 cagcaccaac accgctgcgc cggccggcct gatcgccggc actcccccgt cgaaccgcat
     2641 cgttcttctc cctggccgcc gatcacggcg gcgagagaac cggcgtgcaa cccgcgcgcc
     2701 agccagatac gcgcggccgc gatgacggtt tcgtccctcg cgcccgtcga aatcccggca
     2761 cgccgttggc cggcatcgct tccaccgggc gccgcgagcg ccgcgcggct ggcagccagt
     2821 gtcaccccgc gacgccgtcg ccgacacgca gcgtcgctcg cgccgggtgt taccatgacg
     2881 cgatgacctt cctcgaacgg ttgctgtcgg acgacccgtt gttgtcgatc gagatgcgcc
     2941 cgccgcgcgc cgacctgtcc gccgaagcgt cgatggactc gtggctcgcg atggggagcg
     3001 ccgtgcgccg gctggcgacg aaggacacgg cgttgttctt taccgacaac gccgtcggcg
     3061 ccaacgaaga agagaatctg caccacctcg tcaccaacct cgaagacgag gtcgcccggt
     3121 tgcggatctg tccgttcctc accaccaagc acacgctcga gtactgcctg tggtacgccg
     3181 accgggcggt ctacgacggc cacaccgctc tgacggtgct cggcggggac cgccacgtcg
     3241 gcccgccgcg ctgcgtgccg cactcggacg agctgcgccg gcgcattcgc cagcggcacc
     3301 gcgggctcgc gctcggcgcc tgggccaacc cgtaccacga cgcagcggcg caggtcggct
     3361 acctggccga cggacgggcg accgccgact tcttcctgtc ccaggtcgtc tcgcaccacg
     3421 agctcggccc ggtcgagcgc ttcctcgaac gggcggagcg cgaagggctg gcgctacccg
     3481 gcgtgttcgg cgtgttctac tatcgcagcg cgaatccgcg gacgttcgcg ctgctgtcga
     3541 gtttcctgcg tgtcccggcc gaggagatca cgcgcgactt cgagcagcgg ggcaaatcgg
     3601 ccgacgagat ctgcgccgag acgatccgcg cgctacgccg gctcggcgtg cgccacgtct
     3661 acatcagcaa tctcaagccg gatgaagcgc cgaagcggct cgcggcgatc ggagcgatcg
     3721 tccgcgccgg ctaggagcct gtccgagtat tgcctacggc cgcgttccgg ccgccgcggg
     3781 gccggatgca aggcgccgcg ctgcagacag atgcgacggc atcggccagg cgcggcaacg
     3841 ccgcagacgg ccccgcgccg ccggaacccg tcgggcgcgt gggggttgcg ctcgcagtcg
     3901 tcgttgctcg tcgtcgacga tgcccgagca tcgacttcct cctcgcgcct cggctgcgag
     3961 cgcaaaaccc acgcttgtcg gcctagagcg cctcgagcac ctcgagcgcc gtctcgagtc
     4021 gcccgccggg aacgtagacc tgcaggccgt ggacgaggtc gcgcacgccg acggtcagct
     4081 cgcgcgcgat cgccacgccc tcgcgcgcct cggtgccgtc gcgctcggct cgctgcatgc
     4141 gctcgacgat cgtcttcggc accggtacgt cgaccatctc gtgttcgaag aactcggcct
     4201 cgcgcgccga acgcaacggc cacaccgtcg cgatgatcgg gatcttgaac tcgcgcgtgt
     4261 cggcgaccag cgactgcagc gcgtccaggt cgaacaccgg tgaggtcaag gcgtactccg
     4321 ccccggcatc gaccttccaa tacaaccgcc ggacctcctg ctcgcggtcg taggacgtcg
     4381 ggtcgagacg cacgccgatg tggaagccgg tcggcttgcc gatcggattg ccgccgatgt
     4441 cctcgccgtg attcagccgc gaggcgaggt tgaccgcgcc gatcgcgtcc acgtcgagct
     4501 cgctcccgcc cgcgtcgagc ggagaatgcg gcggctcgcc ggtgaccaac agcaggttgg
     4561 ccagtccgct ggcccagacg ccgagcaggt cggactggat ccgggccaga cgccgctcgc
     4621 ggcaggcgta gtagaccacc ggctcgatgc cgcggaccgg cgcgagcagc tgggccatcg
     4681 cgtacggcgg catccgcgcc tcgctgcggg agtcttcggg aagggcgagg aagctggccc
     4741 cggccgcggc cagccgccgc gccgcggcga cgatgcccga cgtctcccaa ccgatcggca
     4801 gcggcatctc ggcaccgacg acgaagcggc tgctggccag cgccccggcg agcaccgagc
     4861 gttccttgcg cgacggcggc tcggcggcgg gtgcgatctc gatcgcgcgc ccgaccgacg
     4921 cgggcggcgt caccggctcg gtttcccgca ccgcttggca cagcatgcgg atgtggtcgg
     4981 gagtcgtccc gcagcaaccg ccgagcacgc gggcgccgac gcgcagcgcg cggcgggccc
     5041 aggcgacgag gtagtcgggc gaggcgaggt agagatgtcg accttggacg gtccgcggct
     5101 ggccggcgtt cggctgcccg gaaagcgggg cgtcgaccgc cttgcgcatc gcctccagcg
     5161 cgtcgagcgt ggccagcgcc tcgctgcagt tcaccccgac cacgtcggcc tcggccgcca
     5221 ccagcttggc cgccgcctcg ccggcgtcga cgccctcggg cgtcacgccg cccttgccga
     5281 ccgtcatctg cgcgatcagc ggcagatccg acacctcgcg cacggcgaag atcgcctgct
     5341 gcagctccga caggttcgag aaggtctcga tcacgaacag gtcgacgccg gccgcggcga
     5401 gagcccgcgc ctgctcggcg aagaccgcgc gcgcctcctc gcgcgaaatc gggccgaacg
     5461 gctcgatgcg gacgccgagc ggaccgatcg cccctgccac ccagtgagcg ccgctccccg
     5521 cctcgcgcgc gatctcgacc gcgcgcgtgt tgatctcttc gacgcggtcg gacaggccgt
     5581 gggccgacag ccggaagcgg ttcgccatgt acgtgttggt ctcgagcacg tcggcgccgg
     5641 ccgcgaggta ttcggcgtgg acctcgcgca cgagcgaggg gcgggtcagg ttcagctcct
     5701 cgaacgcgcg gttgatgaac acgccgcggc tgtagaacat cgtccccatc gccccgtcga
     5761 acagggcgac gccgccctgc tcgagcagcc ggcggaacgg ctcctggccc ggtttgacct
     5821 tcggtttcgt cggggcgtgg cggtccatgc gtgcgcttcc gtccggtaat tgtatcgacc
     5881 cggtgcgggc cgcgcagtgg cgggacgtgt catagcctcg agcgcgccga aacggcggcg
     5941 gttgcggcta tcatcgcggt gatggccagc gatcgttcgg tacggatcgc catgtggtcg
     6001 ggtccgcgga acatctcgac cgcgatgatg cgctcgtggg gcaatcgccc ggacaccttc
     6061 gtctgcgacg agccgctcta cgcccactac ctgctcagca ccggtctgtc ccacccgggc
     6121 gccgacgagg tcatcgcccg ccacgagacc gactggcggc gagtggtcgc gtggctcacc
     6181 gggccggtgc cggagggccg gacgatcttc taccagaagc agatgaccca ccacctgttg
     6241 cccgagatcg accgcgggtg gttggacgag ctgcgtcacg cgttcctgat ccgcgacccg
     6301 ggaccgatgt tggcctcgct ggccagcgtc tacccgcatc cgacgctcga cgacaccggg
     6361 ctaccgcagc aggtcgagat cttcgagcgt gtgcgtcgca cgaccggcaa cctgccgccg
     6421 atcgtcgacg cgcgcgaggt gctggccgat cccgagcgga tgctgcgcaa gctgtgcgcg
     6481 gcgctcgggg tcgagttcac cccgcggatg ctgagctggg ccccgggtcc gcgcgcgacg
     6541 gacggggtct gggccaagca ctggtacgac gcggtggtca agacgacggg cttcctcccc
     6601 gccgccccgc gcgagcggcc ggcgccaccg gggatggacg acctgctgcg cgaatgccgc
     6661 gagccgttcg agctgctcta ccgccaccgc ctgcggccgt agccggatcg gcgcggccga
     6721 ggagtcccgc atgctgcaat cattcgacga gaggaatcgc gacctcgaga tcaacgtcaa
     6781 cgggcgtctc gtgcatcgcg acgaggccgg gatcagcccg ttcgactccg ccgtccaggg
     6841 cggcgacgcg gtgtgggagg ggctgcgcct gtacaacggc cgcgtgttca agctgcgcga
     6901 gcatctgacc cgtctgcgcg gctcggcgcg ggcgctcggg ttcgagtcga taccgagcga
     6961 cgaggcgatc atcgaggaga tccggcgcac gctggtcgcc aaccggatgt tcgacggcgt
     7021 gcacatccgg ctgacgctga cccgcggcgt gaagatcacc tcgggcatgg acccgcggct
     7081 caacacccgc gggccgacgc tgatcgtgct cgccgagcac aagccgccgg tctacgccaa
     7141 gacgggactg acgctgatca cctcgagcgt ccgtcgtgcc ccccccgact gcctcgaccc
     7201 gaagatccac cactgcaacc tgatccagtc gatcctggcc aagatcgaag ccaacgccgc
     7261 cggggcggac gacgcggtga tgctcgatcc ccgcggcttc gtcgccgaga ccaacgccac
     7321 ccacctgttc ctggtcgagc gcggcacggt cgccaccccg cggaccgtgg cctgtccgga
     7381 aggggtgacg cgtgccaccg tgctcgagct gtgcgccgcc gaaaggatcc cgacgcgggt
     7441 cggcgatctg tcgctggccg agttctatcg ggccgacgag gtattctgca gcgggacgat
     7501 gggcgagctc gccgcggtcg tcgcgatcga cggacgccgg atcggcggcg gcgaaccggg
     7561 gccactgacg ctgcggctgg acgcggccta tcgctgcgaa accgccacgg gcggcgagcc
     7621 gctggtcgat cgggcgtgac gccccggttc aaacctcggg ccgctcgcga gcatccttat
     7681 cgtcggagcg aggcagcccg ctcgacggga gcacgaccgt atggaggtct tggccatccg
     7741 cgcaccgggg aatcacggcg cgattgaccc gctcgtcgcg cgagccaagg ccggcgactt
     7801 cggcgcgttc gaggggctgt acaacgcgca cctgcacggg gtctacgcga tctgcatgcg
     7861 gatgacggcg aaccggtctc gggccgagga gctgacgcaa gagaccttcg tccgtgcctg
     7921 gcagcggctc gcccagttcc gcggcgaggg cggcttcgcc ggttggctgc ggcggatggc
     7981 ggtcaacgtg gtgctgggcg actcgcgcgc caccgcgcgc cgtcggcgtc gcgagacggt
     8041 gcccgagcgg acgcccgcct ttcccggtgc cgccgccgcg cacgagccgg cccgggcgat
     8101 cgatctcgag cacgcgatcg aacgcctgcc gacccgcgcc cggcacgtct tcgtgttgca
     8161 cgacatcgag ggttaccggc acgaggagat cgcgcgcagg ctcggtgtcg cggtcggtac
     8221 ctcgaaggcg cagctccacc gggcccggaa gctgctgagg gaggcgctga gagaatgaac
     8281 tgcatcgatt acgagctgct gctcgacgat tacgtcgacg gcgagttgga cggcgcgcgc
     8341 cgggccgagg tcgaacggca cgtggcgacc tgcgcggctt gccgtgccgc gctcggcgag
     8401 ctcggtcagc tcgcgtcggc ggtcggcgaa ctgccccgcg ggctcgcccc cgagcgggac
     8461 ttgctgcccg gcatccgccg gacggctctc ggacggggcg gggcgaggtg gcggctgtgg
     8521 gccgggttgg ccgcatccgt cgccctcgcc gcgaccgcgg tctggatcgg tctgcactac
     8581 gccggccgac ccgggacgcc gccggaccgg gagccgggtc ggctgagcgc ggatcgcgag
     8641 cgcgcgtggt ccgtggcaca ggtgactcgc gccgagttcg agctggccga gcaggagtac
     8701 gaggaggcca ccgcgcgact gctgcgcctg ctcgacgcgc gggccgactc cctttcaccc
     8761 gagacccgcg agctggtcgc ggagaacctg cgtatcatcg atcaggcgat cgccgagctg
     8821 cgtaccgcgc tcgacaacga tccgaacgat gcgcgcaacg ggcacgcgct caactcgctc
     8881 taccggcaca aggtcgagtt cctctggctt atgtcccggc tgtcgtcctg acgacgatgc
     8941 ccaggagatg aaggtgatgc gtaggattgc caagacatgg accacgttga tcgtcgcctt
     9001 gggctgcgtc gcggccgtcg ccggcgagcg tcaggtcgac cagacgatcg aagccgcagc
     9061 cgacggcacc gtcggcatcg agctgatctc ggggtcggtt cgcttcgtcg gctggagcgg
     9121 cgacgagatc cggatcacgg ggaccctcgg gcgcgacgtc gaggggctcg acgtgcgcaa
     9181 gagcgacaac tcggtccgga tcgaggtcga gctggtggag cgctccggcg ggatgcacga
     9241 ggccgacgcc gatctcgaga tccgcgtgcc ggcggacagc cgcatcgagg ccgagtcggt
     9301 cagcgccgac ctcgagatcg agggtatccg cggggaggtc tcgatcgagt cggtgaacgg
     9361 cgaggtgcag atccggggcg aggtgaccga ggccgaggtg gcgacggtgt cgagcgacgt
     9421 ccacgtcgcc tccgacggcc gactgcggga cggacggttc gagacggttt ccggcagcat
     9481 cgagttcgag ggggcgctcg acccgaccgg acggttcgac ttcgaggcgg tcagcggcga
     9541 catcacgttg cggatccctg cggggacggc ggcggatttc gacgtcgaga ccttcagcgg
     9601 gacgatcgtg aacgagctgg gaccggaacc gcgccgcacc agcgaacacc tgccgtcgaa
     9661 gtcgctcgcg ttctcgacgg gtgcgggcgg ggcgcgggtg tcgatcgagt cgttcagcgg
     9721 tacggtgcgc atcctcggcc gatgacgtcc ggacgagcga cggatccgtc ggatggatag
     9781 aatggtgcgt ctgctgacgt agcgtcgaat ttgaaccagg ccaagcagct caagcactac
     9841 gagatcctct cgcgcatcgg cgaggggggc atgggcgtgg tctac
//
LOCUS       DASPGN010000431         9857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_430599, whole genome shotgun sequence.
ACCESSION   DASPGN010000431 DASPGN010000000
VERSION     DASPGN010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9857
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430599"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VD788_12520"
     CDS             complement(<1..289)
                     /locus_tag="VD788_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77133.1"
                     /translation="MGAASSTDFGITWLAYALMTAASWGLYGIFLHSGQVAMGDPING
                     RYKAFLLVGVAYFVTAVLAPLFMLLSAGSDWNLPAKGLWLSLVAGTVGAIGA"
     gene            528..1640
                     /locus_tag="VD788_12525"
     CDS             528..1640
                     /locus_tag="VD788_12525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV77134.1"
                     /translation="MSGIIKLLFGVVVLAAVVFGGYSWLQGREPAGSGLETFPVELGS
                     ITEKAVAVGQIEPRIQFRVKSKISGIVRKCSVEIGDRVLPGDALFEIAPDPTPSELVE
                     AESQVDAGRSGFHRAEADWKRSQALASQGVISSDQLDRDREAYELARIELERAEDNLE
                     LTRAGRVSGRGRTMESIIRAPAGGIVLARSVDPGDPVVPLTSFQEGTELATIADMRDL
                     VFKGTVDEIDVGKLAPGASARLKIGALPEQTVTGTLTRIAPQAKEEDGARLFEVEIEL
                     DPNEDVVLRAGYSANADLIIREKTDILLVPERLVLFEDDGARTFVEVPDAADPLAEPK
                     KVEIETGLSDGLNIEVTSGLDEGDLVVQRPPRDVLG"
     gene            1647..2876
                     /locus_tag="VD788_12530"
     CDS             1647..2876
                     /locus_tag="VD788_12530"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV77135.1"
                     /translation="MPWRNTIRQLARDLKSQKLRTFLTTFGIVWGTVAVSLLLAFGEG
                     FHHQLRKTSAGLGKGIVICWPSLTSIPFEGLGKGRRIRLDETDIDLIRSKALGVDSIS
                     NEYADNLRLQLGPRTLSVDVSGVRPAFGEMRNMIPAAGGRFLNPIDEEQKRRVAFLGD
                     ELAQTLFGGADAVGQQIRVHGSPFTVVGVLQPKTQDSSYSGRDKDKVIVPGSTFRALT
                     GQKYLDNFIFMARDLSRTEQTTAEVLSIIAGKHRFDQTDKEALSVWDTSEGFRFLDTF
                     MFAFKLFLGVVGSLTLVVGGIGVSNIMNVVVEERTREIGIKMALGATPGAVLRQFMAE
                     TVVMTAAGGTLGLSLTHAICSAFPAFGLTEYVGTPTVSLTVGGATAALLGAIGLVAGY
                     FPARSAARLDPVVAMKT"
     gene            2876..4132
                     /locus_tag="VD788_12535"
     CDS             2876..4132
                     /locus_tag="VD788_12535"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV77136.1"
                     /translation="MFTRPLLVVQLFLRSSRVQKKRAILTIAAIAWGSLSLLLLLAFG
                     EGLKRQLTLANAGMGQDIAVLWPGQTTRPWNGLPAGRSIRPRVEDVELLASRVEHLEA
                     VTGELRTWRAAYTYGKRTVNGRLTGVHLAFGEMRNHIPESGGRWLNPLDEQLRRRVIF
                     LGFELAEDLFGDEDPIGRTLQVNDVPFTVVGVMKHKLQMGTYGGPDASHGVIPITTFK
                     TQLGRDRLDVIVVKPKEPQLMPAVLRQVRETLGAKYGFDPGDPRAIGTWDTVEGAAML
                     RNMTLGIQLFLGIIGALTLVVGGIGVANIMYAVVKERTREIGVKMALGARRSWITAPL
                     VLEGLTYTMLGGLLGMLMATCLVAGLSLIPTAGNEAIEMLGKPTLSLEIAMANAIVLG
                     AIGLMAAYFPARRAASVDPAETLRYE"
     gene            4125..4853
                     /locus_tag="VD788_12540"
     CDS             4125..4853
                     /locus_tag="VD788_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV77137.1"
                     /translation="MSEAMQTARRAGPTTSDKVIRMEQIRKVYDTGKVRVEALCGVDL
                     SAMQGEFIAIVGPSGSGKSTLMNLIGCLDTPTSGVYELRGEKVAGLRREQLAEIRNRR
                     VGFVFQAFNLLPQISAFENVEMPMMFGGVPRRRRRERVEALLERVGLQDRMTHRPTEL
                     SGGQMQRVAIARAMAMEPDILLADEPTGNLDTSSGGDIMTLFTELWQQGCTIVVITHD
                     RALADRAPRQVEIRDGRITSDVRA"
     gene            complement(4805..6652)
                     /locus_tag="VD788_12545"
     CDS             complement(4805..6652)
                     /locus_tag="VD788_12545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77138.1"
                     /translation="MSVAGEVCEESVPIAPSPQPNSRERWLPLPLLAALTAVGVALLF
                     RTPQWIASHDLDRYPVLVDAFGRSFLAGNLYPRWLPDLFGGYGYPTFVYYQPGYFFLT
                     LPFYLLVPDTILALKLGITTLLFGGVYGFYLLARRLADWRTALFAAACFGMTPYIYVN
                     LYVRGDLSELAAMLLAPWPLYFLVRTARPRNGARTPRLDVAGMTVALAAVIASHPAVA
                     ICYLPAFCLFALGLALPLRRGARGLAAAAIAVIASVALTAPYWFMTLTMKRHVHFERL
                     IEGYNQARSHTVQFEQLFSRLWGFGSSTPNSPADGMSFQLGLPLCLLALGGAWIGRRD
                     RAVRWAFAIYLLLVAAMTPLASSLWSIPSPYQYLQFPWRLLSTIAVFQALVVVGLAGL
                     GGQGGNGHRHAAVLALAWGLLFVWSANQFRLGGEFDPRLTVEGRDQRMAGFKTFANSN
                     EFSPRTADPPPTVPREANRMIVVPRSVAIEPGPGHGVHSLDYRIESPHRSTEIFIQQL
                     YFPGWAVEIDGRPVADVELRNTRLPDGRMRVWLEGPGSHRLVARYAGPPGQGRLSAIS
                     IGWILLMAVGLARFGDRLVVPHGPSDPGAIDDERQARTSLVIRPSRIST"
     gene            complement(6710..7186)
                     /locus_tag="VD788_12550"
     CDS             complement(6710..7186)
                     /locus_tag="VD788_12550"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV77139.1"
                     /translation="MATKPIPEGFNTVSSYLVVSDAAKAIEFYAKAFGAERVNHMPMP
                     GGKGTMHAEMRLGDSRFMLTDENPQWGTRSPKSLGGTPVSMHIYTRDADAMFKRAVGA
                     GCEVQMPPTDMFWGDRYAKVADPFGHVWGIATHVEDVPDAELGKRAKKWFADMAGQ"
     gene            7354..8832
                     /locus_tag="VD788_12555"
     CDS             7354..8832
                     /locus_tag="VD788_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77140.1"
                     /translation="MTGKTDDAPRRPGLPLLLAVAAAAVYLPSLFAGFFSDDFQWLGR
                     MNATLERPLYLFSIIYRDFNPVLHASLLLDWLAGRGSPVAFHAQSILIHAANAALLFV
                     LCRKLALPSSGAAAVTLLWAWNVRISEAVIWPAARGHELATTFVLAAFVVLLGSLRWR
                     SALALSLFVAALLTKETALMPMLLVPMLLGASRRHWRLYAAVGSLGFAFVLFNLVAKP
                     DFHSSPAPLSALLLKIPFILLRPIGLGDHYDFSPPMAVALLVLFGGAAWLLRRTPAAV
                     GFAWVALCTLPIIPLDKLSSRYLYMMAVGYALVAYGAAEWSVRSLTAASARAVLRGAM
                     TIGVGVVLAAGALRIQAEIRDYEDLGRPYHSCLSILAPELVALEPGEQVVVVDVGPRD
                     SVRRLADELNARPTITKLIPSRDAGVDGLIALVDMLNVARERSPGLLGYAGRLGEPGP
                     TRFVVFDGHAARRVAPIPTDRLPDGRVFVAGWGEADRYFEAD"
     gene            complement(8858..9511)
                     /locus_tag="VD788_12560"
     CDS             complement(8858..9511)
                     /locus_tag="VD788_12560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77141.1"
                     /translation="MKQPRSIPAAVLPAATLALSLACNVSVNGPIHVADGETRENGLA
                     TVNGSLTVGRDCRIAGACSVVNGEIEIGEGSSVGGLNAVNGPIRVGPRVTVDGDVDSV
                     NGPISLASGTIVGGEVSTINGPIELDGTTVGEHVRTVNGDLQLSRSSVVKGNVVIERK
                     IGGVRADRPLTVLIDTGSVVEGDLIVEDPDLPVKVYLRHGGRVNGEIRNAEVVRDAT"
     gene            complement(9531..>9857)
                     /locus_tag="VD788_12565"
     CDS             complement(9531..>9857)
                     /locus_tag="VD788_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77142.1"
                     /translation="LDRKLNRKASKGWIASRAGSTETVLTLERMRGEAADATSAQDVF
                     LALTSTRSDFSSRMARAVDAGYRYLGLFVEAGQTTVLLQKQPDSTASARSSTRREPEA
                     DPGRSP"
BASE COUNT         1634 a         3300 c         3294 g         1629 t
ORIGIN      
        1 aggcgccgat cgccccgacg gtcccggcga ccaacgacaa ccacagcccc ttggccggca
       61 ggttccaatc cgagcccgcc gacagcagca tgaacagcgg ggcgagcacg gcggtgacga
      121 agtacgcgac gccgaccagg aggaacgcct tgtagcgacc gttgatcgga tcgcccatcg
      181 ccacctggcc cgagtgcaag aagatgccgt agaggcccca gctggccgcg gtcatcaacg
      241 cgtaggccag ccaggtgatc ccgaagtctg tagagctagc ggctcccatc cgttccctcc
      301 gctggggtga ttccgcgccc cggtccaacc ccgcgtccgc tcgcggcatc tggatcgaac
      361 gcgccccgag gacgaaccac cggggcgccg gccggggcct cggtacagtg ccgaattacg
      421 atagcctaac cgcttggccg ctgccgtcca gccggccaga aacttttgac cgggtccgac
      481 gttattctca gagaaaggtg gcgcccggcg gcgacggggg gttgaccatg tccgggatca
      541 tcaagctgtt gttcggggtc gtcgtgctgg ccgccgtcgt cttcggcggc tacagctggc
      601 tccaagggcg ggagccggcc ggaagcggtc tcgagacgtt cccggtcgag ctcggatcga
      661 tcaccgagaa agccgtggca gtgggccaga tcgagccacg gatccaattt cgcgtgaagt
      721 cgaagatctc cgggatcgtc cgaaagtgtt cggtcgagat cggggaccgg gtcctgcccg
      781 gagacgcgct gttcgagatc gccccggacc cgacgccctc cgagctggtc gaagcggaaa
      841 gccaggtcga cgcgggacga tcgggctttc atcgagccga ggccgattgg aagcgatcgc
      901 aggcgttggc gtcgcagggc gtgatctcgt ccgatcagct cgaccgggac cgcgaggcct
      961 acgagctggc ccggatcgag ctcgagcggg ccgaggacaa cctggagctg acccgtgccg
     1021 ggcgcgtgtc cggccgtggc cggacgatgg agtcgatcat ccgcgccccg gccggtggca
     1081 tcgtgttggc gcgcagcgtc gaccccggcg acccggtcgt gccgctcacc tcgttccagg
     1141 agggcaccga gctggccacg atcgccgaca tgcgtgacct cgtgttcaag ggcacggtcg
     1201 acgagatcga cgtcggaaag ctggcgcccg gcgcgtcggc gcgtctgaag atcggcgcgc
     1261 tgccggagca aaccgtgacc ggaacgctga cccgcatcgc gccccaagcc aaggaagagg
     1321 acggcgctcg gctgttcgag gtcgagatcg agctcgaccc caacgaggac gtcgtgctgc
     1381 gcgccggtta ttcggccaat gccgacctga tcatccggga gaagaccgat atcctgttgg
     1441 tgcccgagcg gctggtgttg ttcgaagacg acggcgcccg cacgttcgtc gaagttccgg
     1501 acgcggccga cccgcttgcc gaaccgaaga aggtcgagat cgaaaccggg ttgtcggacg
     1561 ggctgaacat cgaggttacc tccggtctcg acgagggtga cttggtcgtc caacgcccgc
     1621 cgcgcgacgt gctcgggtag tcggccgtgc cgtggcgcaa cacgatccga cagttggcgc
     1681 gggatctcaa gagccagaag ctccggacat tcctcacgac cttcggcatc gtctggggta
     1741 ccgttgcggt cagcctgctg ctcgcgttcg gcgaaggatt ccatcatcag ctgcgcaaga
     1801 cctccgccgg gctgggtaag gggatcgtca tctgctggcc gtcattgacc tcgattccgt
     1861 tcgagggact gggcaaggga cgacgcattc gcctcgacga gaccgacatc gacctgattc
     1921 gcagcaaggc gctcggcgtc gacagcatct cgaatgaata cgcggacaac ctccggctcc
     1981 agctcggtcc gcgcacgctc agcgtcgacg tttcgggcgt gcgtcccgcg ttcggtgaga
     2041 tgcgcaacat gatcccggcc gcgggaggcc ggttcctcaa cccgatcgat gaggagcaga
     2101 agcgacgagt cgcgtttctc ggcgacgagc tcgcgcagac gttgttcggc ggcgccgacg
     2161 ccgtcggcca acagatccgc gttcacggat cgccgttcac ggtggtcggc gtgctgcaac
     2221 cgaagaccca ggactcgtcg tacagcggcc gcgacaagga caaggtcatt gtgccgggat
     2281 cgacgtttcg agcgctcacc gggcagaagt acctggacaa tttcattttc atggcgcgcg
     2341 acctgtcgcg caccgaacaa acgaccgccg aggtcctgtc gatcatcgcc ggcaaacacc
     2401 gcttcgacca gaccgacaag gaggcgctgt cggtgtggga cacgtcggag ggcttccgct
     2461 tcctcgacac gttcatgttc gcattcaagc tctttctcgg cgtcgtcggc tcgctgacgc
     2521 tggtcgtcgg cgggatcggc gtgtcgaata tcatgaacgt cgtcgtcgaa gagcgaactc
     2581 gggagatcgg catcaagatg gcgctcggtg ccacccccgg cgccgtgctg cggcagttca
     2641 tggccgagac ggtggtaatg acggccgccg gtgggacgct cggtctgtcg ctcacgcacg
     2701 cgatctgctc ggccttcccg gccttcgggc tgaccgagta cgtcggtacg ccgaccgtct
     2761 cgctgaccgt cggcggcgcg acggccgcgt tgctcggtgc gatcggactc gtcgccggct
     2821 attttccggc gcgcagcgcg gcgcgtctcg acccggtcgt cgcgatgaaa acgtgatgtt
     2881 cacccggccg ctgctcgtcg tacaactctt cctgcgatcg tcgcgggtcc agaagaagcg
     2941 agcgattctc accatcgcgg cgatcgcgtg gggctcgttg tcgctgctgc tgttgctggc
     3001 gttcggcgag gggctgaagc ggcaactcac gctggccaac gcggggatgg gccaggacat
     3061 cgcggtactg tggcccggcc agacgacccg accgtggaac ggcttgcccg ccggccggtc
     3121 gatccgaccc cgcgtcgaag acgtcgagtt gcttgcctcg cgggtcgagc acctcgaagc
     3181 ggtcaccggt gagctgcgca cctggcgcgc cgcctatacg tatggcaagc gcacggtcaa
     3241 cggccgattg accggcgtgc atctcgcctt cggtgagatg cggaaccaca tccccgagtc
     3301 cggtggacgc tggctcaatc cgctcgacga gcaactccgt cgccgcgtga tcttcctcgg
     3361 cttcgagctg gccgaggacc tgttcggaga cgaggatccg atcggtcgga cgttgcaggt
     3421 caacgacgtg ccgttcacgg tcgtcggtgt gatgaagcac aagctgcaga tgggaaccta
     3481 cggcgggccg gacgccagcc acggcgtgat accgatcacg acgttcaaga cccaactcgg
     3541 tcgcgatcga ttggacgtga tcgtcgtcaa gccgaaggag ccgcagctga tgccggccgt
     3601 gctccggcag gtgcgcgaga cgctgggcgc gaaatacggc ttcgatcccg gggacccgcg
     3661 ggcgatcgga acctgggata cggtcgaagg ggccgcgatg ctgcgcaaca tgacactcgg
     3721 tatccagctg ttcctcggca tcatcggtgc gctgaccctg gtcgtcggcg ggatcggcgt
     3781 cgccaacatc atgtacgccg tcgtcaagga gcggacgcgc gagatcggcg tcaagatggc
     3841 gctcggcgcc cggcggagct ggattaccgc acccctcgtt ctcgagggac tgacctacac
     3901 gatgctcggc ggcctgctcg gcatgctgat ggccacctgt ctcgtcgccg ggctctcgct
     3961 gataccgacc gccggaaacg aggcgatcga gatgctgggc aagccgaccc tgtcgctcga
     4021 gatcgcgatg gccaacgcca tcgtgctcgg agcgatcggg ttgatggcgg cctacttccc
     4081 cgcgcgccgt gcggcgtcgg tcgatcccgc ggagaccctg cgctatgagt gaagcgatgc
     4141 agaccgcccg acgcgccggc cccacgacct ccgacaaagt catccggatg gaacagatcc
     4201 gcaaggtgta cgacacgggc aaggtcaggg tcgaggcgtt gtgcggcgtg gacctgtcgg
     4261 cgatgcaggg ggagttcatc gcgatcgtcg gcccgtcggg atcgggcaag tcgacgttga
     4321 tgaacttgat cgggtgtctc gacacgccga cctcgggagt ctacgagctg cgcggcgaga
     4381 aggtcgccgg cctgcggcga gagcagctcg cggagatccg caaccggcgg gtcggtttcg
     4441 tgtttcaggc gttcaacctg ctgccgcaga tctcggcctt cgagaacgtc gagatgccga
     4501 tgatgttcgg cggcgtgccg cgccggcgac ggcgcgaacg ggtcgaggcg ctgctcgagc
     4561 gcgtcgggct gcaggaccga atgacgcaca ggccgacgga gctgtctggc ggtcagatgc
     4621 aacgcgtggc gatcgcccgg gcgatggcga tggagcccga catcctcctc gccgacgagc
     4681 cgaccgggaa tctcgacacc tcgtcgggag gcgacatcat gacgctgttc accgagctgt
     4741 ggcagcaggg gtgcacgatc gtagtcatca cccacgatcg cgccctggcc gaccgtgctc
     4801 cacgtcaggt cgagatccgc gacggacgga tcacgagcga cgtgcgcgcc tgacgctcgt
     4861 cgtcgatcgc acccgggtcg gacggaccgt gcggtaccac caaacggtcg ccgaaccgcg
     4921 ccagcccgac cgccatcagc aggatccagc cgatcgagat cgccgacagc cggccctgac
     4981 cggggggccc cgcgtagcgc gcgacgagcc ggtgcgaacc cgggccctcg agccagacgc
     5041 gcatgcgacc gtcgggcaac cgcgtgttgc gcagctcgac gtcggcgacc ggacgcccgt
     5101 cgatctcgac cgcccagccc ggaaagtaca gctgctggat gaagatctcg gtcgagcgat
     5161 gggggctctc gatccggtaa tcgagcgaat gaactccatg acccggtccc ggctcgatcg
     5221 cgacgctgcg gggcacgacg atcatgcggt tcgcctcacg tggcaccgtc ggcggaggat
     5281 cggccgtccg cggcgagaac tcgttcgagt tggcgaacgt cttgaacccg gccatccgct
     5341 ggtcgcgtcc ttcgacggtc aagcgtgggt cgaactcccc tcccagtcgg aactggttgg
     5401 cgctccagac gaacaacaga ccccaggcca gggccagcac cgctgcgtgg cggtggccgt
     5461 tgccgccctg gccgccgagc cccgccagac cgaccaccac cagcgcttgg aagacggcga
     5521 tcgtcgatag cagccgccac gggaactgca ggtactgata gggtgacggg atgctccaga
     5581 gcgaagacgc caacggcgtc atcgccgcca cgagcagcag gtagatcgcg aacgcccatc
     5641 gcaccgcgcg gtcacgacgg ccgatccacg ctccgccgag cgccagcaga cacagcggca
     5701 gcccgagctg gaacgacatg ccgtcggccg gcgagttcgg cgtcgaactg ccgaaccccc
     5761 acaggcggga aaagagctgc tcgaactgta cggtgtgcga tcgtgcctga ttgtaaccct
     5821 cgatcaaccg ttcgaaatgg acgtggcgct tcatcgtcag ggtcatgaac cagtacggcg
     5881 cggtcagcgc caccgacgcg atcacggcga tcgccgccgc ggcgagcccc cgtgcacccc
     5941 ggcgcagcgg cagcgccaag ccgagggcga acagacagaa cgccggcagg tagcagatcg
     6001 ccaccgccgg atgcgaggcg atgacggccg ccagcgcgac ggtcatgccg gcgacgtcca
     6061 ggcgaggagt ccgggcgccg ttccgcggcc gcgcggttcg cacgaggaaa tacagcggcc
     6121 aaggcgccag cagcatcgcc gccagctcgc tgaggtcccc gcgaacgtac aggttgacgt
     6181 agatgtacgg agtcataccg aagcaagccg cggcaaatag cgccgttcgc cagtcggcga
     6241 ggcgccgcgc gagcaagtag aagccgtaga cgccaccgaa caacagcgtc gtgatcccca
     6301 gcttgagcgc gagaatcgtg tcgggaacca gcaggtagaa cggaagggtg aggaaaaaat
     6361 agccgggctg atagtagacg aacgtcgggt agccgtagcc gccgaacagg tccggcaacc
     6421 acctggggta gagattgccg gcgagaaagc tgcggccgaa ggcgtcgacc aacacggggt
     6481 aacggtcgag gtcgtgcgat gcgatccact gcggcgtacg gaagagcaaa gcgacgccga
     6541 cggccgtcag tgccgcgagc aacggtagcg gcaaccaccg ctcgcggctg ttcggctgtg
     6601 gtgacggcgc gatcggcacg gactcctcgc acacctcccc ggcgacgctc acaacgtcgt
     6661 ccgctccgag ttcgatgtca agcagccggc gagtcccgtt cgtcgcgcgt cactgacctg
     6721 ccatatcggc gaaccacttc ttcgcgcgct ttccgagctc cgcgtccggc acgtcctcga
     6781 cgtgggtcgc gataccccag acgtggccga acggatcggc caccttggca tatcggtcgc
     6841 cccagaacat gtcggtcggg ggcatttgca cctcgcagcc cgcgccgacc gcgcgcttga
     6901 acatcgcgtc ggcgtcccgc gtgtagatgt gcatcgacac cggtgtccca ccgagcgact
     6961 tgggcgagcg cgtgccccat tgcggattct cgtccgtgag catgaaacgc gaatcaccga
     7021 ggcgcatctc ggcgtgcatg gttcccttgc caccgggcat cggcatgtgg ttgactcgct
     7081 cggctccgaa cgccttggca tagaactcga tcgccttcgc cgcgtcgctc acgacgagat
     7141 agctactgac ggtgttgaag ccttccggga tcggcttggt cgccatcggg tcctccttct
     7201 gttggatcgc ctctcgagca cgttcgctcc ccgcaggata gaccacaacc gcacgctttt
     7261 catcgcgaga tccgctcgtc gcgcgccgag tttgaccccc ggcgcgcgga cggccatatt
     7321 tttcggcgtc tttcgatcgc gggcgaccac cgaatgactg gtaagaccga cgatgcacca
     7381 cgccgccccg gactgccgct gctactggcg gtggcggccg cggccgtcta cctcccgtcg
     7441 ctgttcgcgg ggttcttctc cgacgatttc cagtggctcg gacggatgaa cgccacgctc
     7501 gagcggccgc tctacttgtt cagcatcatc taccgggatt tcaacccggt gctgcacgcg
     7561 tcgctgttgc tcgactggct ggccggtcgg ggctcgccgg tcgcattcca cgcgcagtcg
     7621 attctgatcc acgcggcgaa cgcggcgttg ctattcgtcc tgtgccgcaa gctcgcgctg
     7681 ccttcgtcgg gcgcggcggc tgtcacgctg ttgtgggcct ggaacgtccg gatctccgag
     7741 gcggtgatct ggcccgcggc ccgcggacac gagttggcca cgaccttcgt gctggccgct
     7801 ttcgtcgtgc tactcggttc gttgcgctgg cgctcggcgc tcgcgctgtc gttgttcgtc
     7861 gcggctctgt tgacgaagga aaccgcgctg atgccgatgc tgctggttcc gatgctgctc
     7921 ggcgcgtccc gccggcattg gcggttgtac gctgcggtcg ggtcgctcgg cttcgcgttc
     7981 gtcctgttca acctggtcgc caagccggac tttcacagtt cccccgcacc gctttccgcg
     8041 ctgctgctca agatcccgtt catcctgctt cgaccgatcg ggctcgggga ccactatgat
     8101 ttttcgccac cgatggccgt cgcgctgctt gtccttttcg gcggcgcggc atggctgctg
     8161 cgccggaccc ccgccgcggt cggcttcgcc tgggtcgcgc tctgcacgct gccgatcatc
     8221 ccgctcgaca agctctcctc gcgctacctg tacatgatgg cggtcggcta cgcgctggtg
     8281 gcgtacggtg cggcggaatg gagcgtgcgg tctctcaccg cggcgtcggc gagagcggtg
     8341 ttgcgtggtg cgatgacgat cggggtcggc gtcgtgctcg ccgcaggcgc gttgcgcatc
     8401 caggccgaga ttcgcgacta cgaagacctc ggccgtccct accacagctg tctgtcgatc
     8461 ctcgcgcccg aactcgtcgc actcgagccg ggggaacagg tggtcgtcgt cgacgtcgga
     8521 ccgcgtgact ccgtccgtcg gttggccgac gagctcaatg ccagaccgac gatcaccaag
     8581 ctgatcccgt cgcgagacgc gggggtcgac ggcctgatcg cgctcgtcga catgttgaac
     8641 gtcgcgcgag agcggtcacc gggcctgctc gggtacgctg gacggctcgg cgagcccggg
     8701 cccacccgct ttgtcgtctt cgacggccat gcggcgcgca gggtcgcgcc gatccccacc
     8761 gatcgcctac cggatggacg ggtgttcgtc gcgggttggg gggaggccga tcgctacttc
     8821 gaggccgact agcgcggatc gccacgcccg ctcgcgctca ggtcgcgtcg cgcaccactt
     8881 cggcgttgcg aatctcgccg ttcacccggc caccatgacg caggtagacc ttgaccggca
     8941 gatcgggatc ttcgacgatc aggtcgccct cgaccaccga acccgtatcg atcagcaccg
     9001 tcagcggccg gtcggcccgc acacccccga tcttccgctc gatgacgacg ttgcccttga
     9061 ccaccgacga acggctcagt tggaggtcgc cgttgacggt tcgcacgtgc tcgccgacag
     9121 tcgtgccgtc gagctcgatc ggtccgttga tggttgacac ttccccaccg acgatcgtcc
     9181 cggacgcgag cgagatcggc ccgttgaccg agtcgacgtc gccatcgacc gtgacccggg
     9241 gtccgacgcg aatcggaccg ttgaccgcat tcagcccgcc gacggacgag ccctcgccga
     9301 tctcgatctc gccgttgacc acgctgcatg caccggcgat ccggcaatcg cgtccgacgg
     9361 tcagtgagcc attgacggtg gccaggccgt tctcacgggt ttccccgtcc gcgacgtgga
     9421 tcgggccatt gacactcacg ttgcaagcca aggacagcgc gagcgtcgcc gccggcagca
     9481 ctgcggccgg aatcgatcga ggctgtttca tcgcaccccc caccccttcg tcaaggagaa
     9541 cggcccgggt cggcctccgg ttcccgccgg gtcgaggacc gggcggaagc ggtcgaatcc
     9601 ggctgctttt gcaacagaac cgtcgtctga ccggcctcga cgaacaaacc gagatagcgg
     9661 tagcccgcgt cgaccgcccg cgccatccgc gacgagaagt cggaccgcgt gctggtcagc
     9721 gcgagaaaga cgtcctgggc ggaggtggca tcggccgctt cgccgcgcat ccgctcgagc
     9781 gtgagcacgg tttccgtcga accggcgcgg gacgcgatcc atcccttact tgccttgcga
     9841 ttcaacttgc ggtcgag
//
LOCUS       DASPGN010000432         5313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1249689, whole genome shotgun sequence.
ACCESSION   DASPGN010000432 DASPGN010000000
VERSION     DASPGN010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5313
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249689"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="VD788_12570"
     CDS             <1..1508
                     /locus_tag="VD788_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77143.1"
                     /translation="GVAGACVALAYAPLAFHAMKLLPVSLAIASQALALALLGAARAG
                     RRAAVPLAGLACGVACLARAEFLLFAPIAFASLWLDRRRPTGARRVAAGLFAAGVLVA
                     IAPATLHNLSHGDRVLIASSAGENLFVGNQRGGNGGHSELHPSAGDLHSQRVWAVRIA
                     ERETGRPLVPSAVSRYWSGRALGEIAADPVAWLALEGRKLMRILDPADPTDMYSLALE
                     RSLYLPVLYLLPLSTTGLWVLGAAGLVGAWRRRREDAWPLLAAVAVQFLVLLAFFVST
                     RLRVPLLFLLTPFAGLAVSSGLARLVERRRVVPLLATAAIVLLGSVHWLVLLRPPPRE
                     AVRLASVLSLRDRLDESLEIVAPWTGPEREDALALDQAGWVHNKKGHLDAARRLYLRA
                     LELGLPSAAREAQTRTRLAALYEREGDVLRATRQHDAAVAAMPDSAPMRHARGLFLLR
                     QGRVREAADDFREAARLAPGWPEPLAMLRSLGLPPPDAGATAEGRAPRQAK"
     gene            complement(1496..2095)
                     /locus_tag="VD788_12575"
     CDS             complement(1496..2095)
                     /locus_tag="VD788_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77144.1"
                     /translation="MSERGLRSATAEPARWYRCSTAARWLPRVIGASLLIATMLGAFR
                     VEARKYTPLPYFQRLVVLAGALVALAVVRRGAELRRSFGLAGDELVVAVGARRSRLSL
                     AEIERLYFAAPFAGTTSWIAATVLVDRDGRQWRLPALIRDGGELVTACLARAGRNDLE
                     AWADAYRVVAKMGRASLHVWVGYATAAALLAVSVAYYFA"
     gene            complement(2092..2811)
                     /locus_tag="VD788_12580"
     CDS             complement(2092..2811)
                     /locus_tag="VD788_12580"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXV77145.1"
                     /translation="MGSLFALADLHLSAAGAKPMHVFGELWRDHAERMARAWDEAVGA
                     DDTVLLPGDLSWARNLSEVAPDLAWIAARPGRKLLLRGNHDSWWSSPGRVRAALPSCC
                     EVLQNNALEFDDWVIVGARGWTDPDDPFAKPGDDRVFARELERLQASVADADARFDRG
                     RPRLAMTHFPPWIDEERPTRVVGLLRRAGVRLCVYGHLHGEDQQRAVRGERDGIRFVF
                     VAADAVRFRPVPIDLAFEPLR"
     gene            2879..4609
                     /locus_tag="VD788_12585"
     CDS             2879..4609
                     /locus_tag="VD788_12585"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77146.1"
                     /translation="MSVRSFHADAAALAAWMLVAAGPSVAAWSAEDLADFAGTEVLPK
                     LGRLVLVYPAGDGEVGRTNRLSADRRAGYLQSELGCEVRTVADTEATEADLSGHLFAI
                     GWSNALLRTPPFESIVRVDPAGGHVFLGEVGVPAGEDLMFSIASPVDPAKRWVFWSRI
                     DPELDRFSVLPILGSDWARYRGYEVTAQGMLVDRSVFPPVYDPHAVGEFDDFRASYPV
                     RARSAHYRLHHADGTLTPAEGQEILTARERALTRAIEVLGRAEPPLEIDLFVYPDTVT
                     KTRASSVPDDVHSVGRSAEMHMLTPYARTPNPHEEMHLLAHRLFGPCVHTALYEGLAL
                     AFDEEASRNREVVAAALVERAGKPTIDALLDENRLRALGKTGLGYPAGGLLASWILER
                     GGVELLGSVYAAEPLSSAELGAKLGQSAADVTASFDAWLVARAERGRDTLAFEEARAR
                     ASSLKRRGDVDGAIVALETALAIRPDDADTLYRLALVEMNGERLSDAEKHLDAIIAQT
                     SRLSSSAARYVTFGHYFRGRVYERTGRPAEARAEFERVLALPDGSDSHRMAREALEKM
                     DEQEAGGGPG"
     gene            complement(4619..4852)
                     /locus_tag="VD788_12590"
     CDS             complement(4619..4852)
                     /locus_tag="VD788_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77147.1"
                     /translation="MSRMIVRRLPLVAASFLLVLFVAERDRRKDDPITYRHAEADMQV
                     LILVFTVVLTMAAALATAAGVLNLFFRVISRRR"
     gene            complement(4973..>5313)
                     /locus_tag="VD788_12595"
     CDS             complement(4973..>5313)
                     /locus_tag="VD788_12595"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HXV77148.1"
                     /translation="LDAPAGDDRQPVAERLPDPTAGDVEDDVARRELQRQVETALEAF
                     RKELGDRDALLLDQRILSEDPLSLQEIGDRFGTTREAVRQAEVRLMKRLSDYLKRELG
                     DLGPIRIGPR"
BASE COUNT          731 a         1939 c         1856 g          787 t
ORIGIN      
        1 ccggtgtcgc cggggcgtgc gtcgcactgg cctacgcgcc gctcgcgttc catgcgatga
       61 agctcttgcc ggtcagcctc gcgatcgcgt cccaggcgct cgcgctggcg ttgctgggtg
      121 ccgcgcgggc cgggaggcgc gccgcggtac cgctggccgg gctcgcctgc ggcgtcgcct
      181 gcctcgcgcg cgccgagttc ctgctgttcg caccgatcgc gttcgcctcg ctgtggctcg
      241 atcgacgacg tccgaccggg gcgcggcggg tcgccgccgg gctcttcgcg gcgggcgtcc
      301 tcgtcgcgat cgcgccggcc acgctccaca acctgtcgca cggcgaccgg gtgctgatcg
      361 cgtcgtcggc cggtgagaac ctgttcgtcg gcaaccagcg cggcgggaac ggcggccact
      421 cggagctcca ccccagtgcc ggcgacctcc actcgcagcg tgtgtgggcc gtgcggatcg
      481 ccgagcgcga gaccggccgg ccgctcgttc cgtcggcggt ctcgcgttac tggagcgggc
      541 gtgcgctggg cgagatcgcc gccgacccgg tcgcctggct cgcgcttgag ggtcgcaagc
      601 tgatgcggat tctcgatccc gccgatccga cggacatgta ctcgctggcc ctcgaacgct
      661 cgttgtacct gcccgtgctc tacctgctgc cgctctcgac gaccgggctg tgggtgctgg
      721 gcgccgcggg tctggtcggc gcgtggcgcc ggcggcggga ggatgcctgg ccgctgctgg
      781 cggcggtcgc ggtgcagttc ctcgtgctgc tcgcgttctt cgtcagtacc cggctgcgcg
      841 ttccgctcct gttcctgctc accccgttcg ccggcctcgc cgtttcgtcc ggcttggcga
      901 gactcgtcga gcggcgacgg gtcgtcccgc tgctcgcgac cgccgcgatc gtgctgctcg
      961 gctcggtcca ctggctcgtg ttgctgcggc cgcccccgcg cgaagcggtc cggctggcgt
     1021 cggtgctgtc cctcagagac cgcctcgacg aatcgctcga aatcgtcgcc ccgtggaccg
     1081 gacccgagcg ggaagacgcg ctggcgctcg atcaggcggg gtgggtccac aacaagaagg
     1141 gccacctcga cgccgcgcgc cgcctctact tgcgcgcact cgaactcggg ctgccctccg
     1201 ctgcccgcga ggcccaaacc cggactcgcc tggcggcgct gtacgaacgg gagggggacg
     1261 tactgcgcgc cacccggcag cacgacgccg cggtcgcggc gatgcccgac agcgccccga
     1321 tgcgtcacgc gcgtggtctg ttcctgctgc ggcagggccg ggtgcgcgag gccgcggacg
     1381 actttcgcga ggcggcgcgt ctcgcacccg ggtggccgga gccgctggcg atgctgcgct
     1441 cgctcggcct ccccccgccg gacgccggcg cgacagcgga aggacgagcg ccgcgtcagg
     1501 cgaaatagta ggcgacggag acggcgagca gcgcggcggc cgtcgcgtaa ccgacccaca
     1561 cgtgcaggga cgcccgcccc atcttcgcca cgacgcgata ggcgtcggcc caagcctcga
     1621 ggtcgttgcg ccccgcgcga gcgagacagg cggtcaccag ttcgccgccg tcccggatca
     1681 gcgcggggag ccgccactgc cgaccgtcgc gatcgaccag caccgtcgcc gcgatccagc
     1741 tcgtcgtgcc ggcgaacggc gcggcgaagt acagccgctc gatctcggcc aagctcagtc
     1801 gggaccggcg cgcaccgacc gcgacgacca gctcgtcgcc ggccaaaccg aacgaccgac
     1861 gaagttccgc gccgcgtcgg acgacggcga gtgcgacgag cgcgccggcc agcacgacga
     1921 gacgctggaa gtacggcagc ggggtgtact tcctggcctc gacgcggaac gcaccgagca
     1981 tcgtcgcgat caacaacgag gcgccgatga cccgcggcaa ccaacgcgcg gccgtgctgc
     2041 agcggtacca gcgcgcgggc tccgccgtcg cggagcgaag gccgcgctcg ctcatcgcag
     2101 cggctcgaac gcgaggtcga tcgggaccgg tcggaagcgg accgcgtcgg ccgcgacgaa
     2161 gacgaagcgg atcccgtctc gctcgccgcg taccgcccgc tgctgatcct cgccgtggag
     2221 gtggccgtag acacacagcc gcacacccgc ccgacgcagc agaccgacga cgcgcgtcgg
     2281 ccgttcctcg tcgatccacg gaggaaagtg ggtcatcgcc aatcttggcc tgccccgatc
     2341 gaagcgcgcg tcggcgtcgg cgaccgacgc ctgcagccgc tcgagctcgc gcgcgaagac
     2401 gcggtcgtcg ccgggcttgg cgaacgggtc gtccggatcg gtccaacccc gcgcgccgac
     2461 gatcacccaa tcgtcgaact cgagcgcgtt gttctgcagc acttcgcaac acgaaggcag
     2521 cgccgcgcgc acacgccccg ggctcgacca ccacgagtcg tggttgccgc gaagcagcag
     2581 ctttcgcccc ggccgcgcgg cgatccaggc caggtcgggt gcgacctcgc tcaagttccg
     2641 tgcccaggac aggtcacccg gcagcagcac ggtgtcgtcc gcgccgaccg cctcgtccca
     2701 ggcgcgggcc atccgctcgg cgtgatcgcg ccacagctcg ccgaagacgt gcatcggttt
     2761 ggcgcccgcg gcggacagat ggagatccgc gagcgcgaac agacttccca ttcggtcgat
     2821 tctagcggac ggcggccgag ccggtggcgc gaccgggtcg ttcacgtaaa atgccgggat
     2881 gtccgtccgg tcgttccatg ccgacgcggc cgcgctcgcc gcctggatgc tggtcgccgc
     2941 gggcccgtcc gtcgcggcct ggtccgccga ggatctcgcc gatttcgccg gcaccgaagt
     3001 gctgccgaag ctcggtcggc tcgtactggt ctaccccgcg ggcgacggcg aggtgggtcg
     3061 caccaaccgg ttgtcggccg accggcgagc cggctacctc caaagcgagc tcggctgcga
     3121 ggtgcggacg gtcgccgata ccgaagcgac cgaggccgat ctgtccggcc acctcttcgc
     3181 gatcggttgg agcaatgcgc tgctccggac ccccccgttc gagtcgatcg tccgtgtcga
     3241 cccggcgggc ggtcacgtct tcctcggcga ggtcggggtg cccgccggcg aggacctgat
     3301 gttctcgatc gcctcgcccg tcgatccggc caagcgctgg gtcttctggt cacggatcga
     3361 tcccgagctc gaccgcttct cggtcctgcc gatcctcggc tcggactggg cgcggtaccg
     3421 cggctacgag gtgaccgctc aagggatgct cgtcgaccgc tcggtcttcc cgccggtcta
     3481 cgacccccac gcggtcggcg agttcgacga tttccgcgcg agctacccgg tccgcgcgcg
     3541 ctcggcgcac tatcggctgc atcacgccga cggaacgctc acgccggccg agggccagga
     3601 gatcctgacg gcccgggaaa gggcgctcac ccgcgcgatc gaggtgctcg ggcgcgccga
     3661 gcctccgctc gagatcgacc tgttcgtcta tccggacacg gtgaccaaga cccgtgccag
     3721 ctccgtgccc gacgacgtgc acagcgtcgg acgctcggcg gagatgcaca tgctcacgcc
     3781 ctacgcccgc acaccgaacc cccacgagga gatgcacctg ctcgcccacc gcctgttcgg
     3841 accctgtgtc cacacggcgc tgtacgaggg gttggccttg gccttcgacg aggaggcgtc
     3901 gcgcaaccgc gaggtcgtcg cggccgcgct ggtcgaacgg gccggcaagc cgacgatcga
     3961 cgcgctgctc gacgagaacc ggctacgggc gctgggcaag acgggcctcg gctaccccgc
     4021 gggcggcctg ctggcctcgt ggatcttgga gcgcggcggc gtcgagctgc tcggcagcgt
     4081 ctacgcggcc gaaccgctgt cgagcgcgga gctcggggcg aagctcggcc agtccgccgc
     4141 cgacgtgacc gcgagcttcg acgcgtggct cgtcgcacgg gccgagcgcg gtcgcgacac
     4201 gctcgcgttc gaggaggcac gcgcgcgggc gtcgtcgctc aaacgccgcg gcgacgtgga
     4261 cggggcgatc gtggcgctcg agacggctct tgcgatccgc ccggacgacg ccgataccct
     4321 gtaccgtctg gcgctggtcg agatgaacgg cgagcgtctc tccgatgccg aaaaacacct
     4381 cgatgcgatc atcgcccaaa cctcgcgcct ctcctcgagc gcggcacgtt acgtgacctt
     4441 cggtcactac tttcgcgggc gggtctatga acggaccggt cggccggccg aggcccgggc
     4501 cgagttcgag cgggtgctcg ccctgccgga cggaagcgac tcgcatcgga tggcgcgcga
     4561 ggcgctcgaa aagatggacg agcaggaggc gggaggcggg cccgggtagc tgcggcgttc
     4621 agcgccggcg ggaaatcact cgaaagaaca gattgaggac gcccgccgcc gtcgccaggg
     4681 ctgccgccat cgtcaggacg actgtaaata cgagaatcag gacctgcata tccgcctcgg
     4741 catgacgata cgtgatcggg tcgtcttttc gcctatccct ctcggctacg aacaatacca
     4801 gcaagaagct tgccgccaca aggggcaatc ggcggacgat catcctgctc aacgtgttag
     4861 cggatatcgt gccggtaccg gcggcggttt caagcggcgc gccccgcggc cgcgcgttcc
     4921 gcttttcgtg cctcgggaga cgtttttgtg gctcacgagc cacgaacgcg tgtcacctcg
     4981 gcccgatccg gatcggaccg aggtctccca gctcccgctt caggtagtcg ctcaggcgct
     5041 tcatcagtcg gacctcggcc tgacggaccg cctcccgggt ggtcccgaag cggtccccga
     5101 tctcttgcag cgagagcggg tcttcgctga ggatccgctg gtcgagcagt agggcgtccc
     5161 ggtcccccag ctccttgcgg aaagcctcga gcgcggtttc gacctggcgc tgcagctccc
     5221 gccgcgcgac gtcgtcctcg acgtcgcccg ccgtcgggtc cggcagacgc tccgcgaccg
     5281 gctgccgatc gtcgcccgcc ggcgcgtcga gcg
//
LOCUS       DASPGN010000433         4773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_196083, whole genome shotgun sequence.
ACCESSION   DASPGN010000433 DASPGN010000000
VERSION     DASPGN010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196083"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VD788_12600"
     CDS             <1..1123
                     /locus_tag="VD788_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77149.1"
                     /translation="APRTLDRLDLQLRLLALAERQLVDCVHDEEVKPWQRLNLARYAR
                     NLALANGGLVPDLFDLVVAARGSVSDNYAWELYHAASVYPAQATSIADLATVRIRADE
                     VFDGTRRTRIRRMVRRPKRPAWRSVLRGKRKTERWPGEWIEGFDGEAICSYPPEDVVI
                     ENFGRYLRRRGKQVLSDERARSVPFTSSVLDGIDVRETIRRWSEGTIFVREAGRVPGE
                     VGSVVMVFDEDDDAYPYRQTWIGEHEQESDMAFYSTEPAQGIVGPGICRVTYGGFMMS
                     YPPQRMLDVWADRDYRIAESSAEILLLAAMDYTEQRYVVHVGPRPPRSMLQQLAARLG
                     LKILHLPLGTLSPPTIRRIRVMHVLSGHDKREIARDYTW"
     gene            1147..1605
                     /locus_tag="VD788_12605"
     CDS             1147..1605
                     /locus_tag="VD788_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77150.1"
                     /translation="MDRKYRQRGYMDGDRDDDRSRGKTAPRKQLTKEERIQQRSLRHA
                     MDREAREVMRCHVCGRSVFDLGTIGRETACPHCNAALHCCRTCVHFDSAARWQCRVPI
                     QQAVPDKNKANECSEYSPRLVLDATGRRSSGPAGGRAGDARSQFEDLFKS"
     gene            1568..2677
                     /locus_tag="VD788_12610"
     CDS             1568..2677
                     /locus_tag="VD788_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77151.1"
                     /translation="MRARSSKICSRARTLAGAVVASVLLVAVIGCVPLAGGDTPQPLL
                     IGRDYPIEAGISFPFALLHWLDSLAGLDGPGMTAGKTIEAHRREYVERLGAPDDATLE
                     QLREFVGLRWSSVKRLGRERRHALSLVFLEARDLDQALAASSELLSDDERDALRRTVE
                     RFAPLYRSIWDDGEVARRFVDGVRGHDAGRALARFLTDVARFYGVPPDRAPHPRIVPL
                     PVVPGYGTHAQAIDRHLLIEIRDGERLLDEIAPIVHENAHFLFYRIDERRRERLEAHA
                     ASIEPGGIEAWRMLHEALPTAIGQGVAVRRFAESAWSMNDRWYHVDEIDAYAKRVYPL
                     VRDRLATGGELDESFIDMAVALYVGERPPITPRSP"
     gene            complement(2658..3074)
                     /locus_tag="VD788_12615"
     CDS             complement(2658..3074)
                     /locus_tag="VD788_12615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXV77152.1"
                     /translation="MKSRTDYLTFTVPRRMGFVNITREVERAVVASGIREGLCLVNAM
                     HITASVFINDDEAGLHEDYQEWLERLAPHDPSRYRHNRTGEDNGDAHHKRQLMGREVV
                     VAVTDGKLDFGPWEQIFYGEFDGRRAKRVLVKVIGE"
     gene            3240..3617
                     /locus_tag="VD788_12620"
     CDS             3240..3617
                     /locus_tag="VD788_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77153.1"
                     /translation="MTTNATSRHHERGEGRVGFLITLIVAAVLIFVGAKVIPVRITAY
                     EFRDALREEARYGAVRNTDALVAQRIMDKAAELEIPLKKTGLTVQRTQSQMVIKAQYE
                     QPIDLKVMTYVYKFDVTEKAPLF"
     gene            complement(3592..>4773)
                     /gene="hutI"
                     /locus_tag="VD788_12625"
     CDS             complement(3592..>4773)
                     /gene="hutI"
                     /locus_tag="VD788_12625"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HXV77154.1"
                     /translation="VRNAAVAVDGDRIVFVGSEREARRSVELDDDAVEIDAAGGTALP
                     GFVDAHTHLPFAGWRETEFDRRLRGATYSSIAAEGGGILSTVSATRRATLEQLAELVR
                     SRLDAMLALGTTTVEAKSGYGLSYAEELKQLAALRDAGADHPLQVVPTFLGAHTVPGE
                     YRDRRDDYVRLLIERILPEVARQRLARYADAFVDAHAFTIDEARRVLTTARELGLGLR
                     LHADQLADDGAAALAAELGAASADHLEFAAAGGIESLARAGTCGVLLPAATFFLMLEA
                     KPPGRRLIDAGVPVVVATDFNPGSCPCESMSAAIWFACLDAGLTVDEAITAATLNAAH
                     SLGMADRVGSLEAGKRADIVVHAVPNRYHLVYRFGVPRVSIVIAGGRPVYDARTTVLS
                     R"
BASE COUNT          767 a         1645 c         1642 g          719 t
ORIGIN      
        1 cgccccgcgc acgctcgacc ggctcgacct gcagctgcgg ctgctcgccc tcgccgagcg
       61 gcagctggtc gattgcgtgc acgacgagga ggtcaagccg tggcagcggc tgaatctcgc
      121 acgctatgcc aggaacctgg ccctggccaa cggcgggctc gtacccgatc tgttcgacct
      181 cgtcgtcgcc gcgcgcggat cggtgtccga caactacgca tgggagctgt accacgccgc
      241 ttcggtctac ccggcgcagg cgacgagcat cgcggacctg gcgaccgtgc gtattcgagc
      301 cgacgaggtg ttcgacggga cgcgacgcac ccgcatccgg cggatggtgc gccggccgaa
      361 acgccccgca tggcggtcgg ttctgcgcgg caagcgcaag accgagcgct ggcccggcga
      421 gtggatcgaa gggttcgacg gcgaggcgat ctgctcttac ccgcctgagg acgtggtcat
      481 cgagaatttc gggcgctacc tgcggcggcg cggcaagcag gtactctccg acgaacgggc
      541 gcgcagcgtg ccgttcacca gctcggtgct cgacggcatc gacgtgcgcg agacgatccg
      601 ccgctggtcg gaaggcacga tcttcgtgcg ggaagcgggg cgcgtgcccg gtgaggtcgg
      661 ctcggtcgtg atggttttcg acgaggacga cgacgcctac ccctaccgcc agacgtggat
      721 cggcgagcac gagcaggaat cggacatggc gttctactcg accgagcccg cccagggcat
      781 cgtcggcccg ggaatctgcc gcgtgaccta cggcgggttc atgatgtcct accccccgca
      841 aagaatgctg gacgtgtggg ccgatcggga ctaccgtata gccgagagca gcgcagagat
      901 cctgctgctg gccgcgatgg actacaccga gcaacgctac gtggtccacg tgggacccag
      961 gccgccgcgc tcgatgctcc agcagctggc cgcccggttg gggctgaaga tcctgcatct
     1021 tcctctcggc acgctttctc cgccgaccat ccggcggatt cgtgtgatgc atgttctcag
     1081 cggtcacgac aaacgcgaga tcgcccggga ctacacctgg tgacgcgcaa cgagcgaggt
     1141 tgaaccgtgg accgtaagta ccgacaacgg ggctatatgg acggcgatcg ggacgacgac
     1201 cggtcacgcg gcaagaccgc gccgcgcaag cagctgacga aagaagagcg gatccagcag
     1261 cggtcgctgc gtcacgcgat ggatcgcgaa gcgcgcgagg tcatgcgctg ccacgtctgc
     1321 ggtcgtagcg tcttcgactt gggcacgatc ggccgcgaaa ccgcctgccc ccactgcaac
     1381 gcggcgctcc attgctgccg gacgtgcgtt cacttcgact ccgccgcacg gtggcagtgc
     1441 cgcgtgccga tccaacaggc ggttcccgac aagaacaagg cgaacgagtg ctccgagtac
     1501 tctccgcgcc tggtgctcga cgcgaccggc cgccgcagct cgggacccgc gggaggccgg
     1561 gccggcgatg cgcgctcgca gttcgaagat ctgttcaaga gctagaacgc tcgccggcgc
     1621 cgtcgtcgcg tcggtcctgc tcgtcgccgt catcggctgc gtaccgctgg ccggaggcga
     1681 caccccccag ccgctgctga tcgggcgcga ctacccgatc gaagccggca tctcctttcc
     1741 gttcgccctg ctgcactggc tcgacagtct cgccggcctc gacggcccgg gcatgaccgc
     1801 cggcaagacg atcgaggccc atcgacggga gtacgtcgaa cggctcgggg cgcccgacga
     1861 cgcgacgctc gaacagctgc gcgagttcgt cggtctgcgc tggagcagcg tgaagcggct
     1921 cggccgggag cgtcgccacg cgctgtcgct cgtcttcctc gaggcgcgcg acctggacca
     1981 ggcgttggcc gcgtcgtccg agctgctgtc cgacgacgag cgcgacgcac tgcggcggac
     2041 ggtcgagcgg ttcgcgccgc tctaccggtc gatctgggac gacggtgagg tggcccggcg
     2101 tttcgtcgac ggcgttcgcg gccacgacgc ggggcgagcg ctggcccggt tcctgaccga
     2161 cgtggcgcga ttctacggcg ttccgccgga ccgcgcgccg cacccgcgca tcgtgccgct
     2221 gccggtcgtt ccgggttacg gcacgcacgc gcaggcaatc gaccgacacc tgctgatcga
     2281 gatccgagac ggcgagcggc tgctggacga gatcgcaccg atcgtccacg agaatgccca
     2341 cttcctgttc taccgcatcg acgagcggcg gcgcgagcgt ctcgaagccc acgcggcgag
     2401 catcgagccc ggcggcatcg aggcgtggcg catgttgcac gaggcgctac cgaccgcgat
     2461 cgggcagggc gtcgcggtac ggcgcttcgc cgagagcgcg tggagcatga acgaccgctg
     2521 gtatcacgtc gatgagatcg acgcctacgc caagcgggtc tatccgctgg tgcgcgatcg
     2581 actcgccacg gggggcgagc tcgacgagag cttcatcgac atggcggtcg cgctgtacgt
     2641 cggcgagcga ccgccgatta ctccccgatc accttgacca gcacgcgctt cgcacgccgg
     2701 ccgtcgaact ccccgtagaa gatctgctcc cacggtccga agtcgagctt gccgtcggtg
     2761 acggcgacga ccacctcgcg gcccatcaac tggcgcttgt ggtgcgcgtc gccgttgtct
     2821 tcccccgtgc ggttgtgccg gtagcgcgag ggatcgtgcg gcgcgagccg ctcgagccat
     2881 tcctggtagt cctcgtgcaa gccggcctcg tcgtcgttga tgaacacgct ggcggtgatg
     2941 tgcatggcgt tgaccaggca caacccctcg cggatgccgc tcgcgacgac ggcccgctcg
     3001 acctcgcggg tgatgttgac gaaccccatc cgccgcggca cggtgaacgt gagatagtcg
     3061 gtcctggact tcatcgacac ctccgtcggg aagcgaacgc cgcggggcga ttctaacgaa
     3121 aacggccgga tttccgcgat ttcctcccga gggcccgtgg cttgtcccgt gttttggccg
     3181 aacctatatt cgaccgggcc ggacggcgcc tcgtccgcgc gaagaggaga gtcgacgcca
     3241 tgacgacgaa cgccacgagc cgacaccacg agcggggtga ggggcgggtc ggtttcctga
     3301 tcacgctgat cgttgccgcg gtgctgatct tcgtcggtgc caaggtcatc cccgtgcgaa
     3361 tcaccgccta cgagttccgc gacgccctgc gcgaagaagc gcgctacggc gcggtgcgca
     3421 acaccgacgc gctcgtcgcc caacgcatca tggacaaggc cgccgagctc gagatcccgc
     3481 tgaagaagac cggactgacg gttcagcgca cccagagcca gatggtgatc aaggcccagt
     3541 acgagcaacc gatcgacctg aaggtgatga cctacgtcta caagttcgac gtcaccgaga
     3601 aagcaccgtt gttctagcgt cgtacaccgg ccggccgccg gcgatcacga tcgacacgcg
     3661 cggcacaccg aatcgataga ccagatgata gcggttcggc acggcgtgca cgacgatgtc
     3721 ggcgcgcttg ccggcctcca ggctgccgac gcggtccgcc atcccgagcg agtgcgccgc
     3781 gttcaacgtc gcggcggtga ttgcctcgtc gaccgtcaaa cccgcatcga gacaggcaaa
     3841 ccagatcgcc gcgctcatcg actcgcacgg acaggagccg gggttgaaat cggtcgccac
     3901 gacgacggga acgccggcgt cgatcagccg tcgcccgggt ggcttggctt ccagcatcag
     3961 gaagaacgtc gcggccggca gcagcacgcc gcacgtaccc gcgcgggcca gcgactcgat
     4021 gccgcccgcc gcggcgaact ccaggtgatc ggccgaggcc gcgccgagct ccgccgcgag
     4081 cgcggcggcg ccgtcgtcgg cgagctgatc ggcgtggagc cgcagaccaa ggcccagctc
     4141 gcgcgccgtc gtcagcacgc gtcgcgcctc gtcgatggtg aatgcgtgcg cgtcgacgaa
     4201 cgcgtcggcg tagcgcgcga gccgctgacg cgccacctcg ggcaggatcc gctcgatcag
     4261 cagccggacg tagtcgtcgc gccggtcgcg gtactcgccg ggcaccgtgt gcgcaccgag
     4321 gaaggtcggg acgacctgga gcggatgatc ggcgccggcg tcgcgcagcg cggccagctg
     4381 tttcagctcc tcggcatacg acagtccgta gccgctcttc gcctcgacgg tcgtcgtgcc
     4441 gagggccagc atcgcgtcga gtcgcgagcg gaccagttcg gcgagctgtt ccagcgtcgc
     4501 gcgccgggtg gcgctcacgg tcgacaggat gccgcccccc tcggcggcga tgctcgagta
     4561 ggtcgcgccg cgtagccggc gatcgaactc ggtctcgcgc cagccggcga acggcaggtg
     4621 ggtgtgcgcg tcgacgaatc cgggcagcgc cgtcccgccg gcggcgtcga tctcgaccgc
     4681 gtcgtcgtcg agttcgaccg accgacgggc ctcccgctcg ctgccgacga agacgatgcg
     4741 gtcgccgtcg acggcgaccg ccgcgttgcg gac
//
LOCUS       DASPGN010000434         2898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_782290, whole genome shotgun sequence.
ACCESSION   DASPGN010000434 DASPGN010000000
VERSION     DASPGN010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782290"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VD788_12630"
     CDS             <1..528
                     /locus_tag="VD788_12630"
                     /inference="COORDINATES: protein motif:HMM:NF045201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77155.1"
                     /translation="WVPPSPNMPSVRTALVYPGGCLIEGTNLSEGRGTTLPFELVGAP
                     WLDPHAVAAGLDSERLAGVLSRPATFRPMFHKHAGIACHGVQLIVTRAEAFEPFLTYL
                     ALIRVVRRLDRRFAWRSAPYEFESERPAIDHLLGVTGLRERLESDEPLERLRSGWEPA
                     LDRFAESRRRYLLYD"
     gene            complement(736..2547)
                     /gene="sppA"
                     /locus_tag="VD788_12635"
     CDS             complement(736..2547)
                     /gene="sppA"
                     /locus_tag="VD788_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HXV77156.1"
                     /translation="MLGPAPPGGGASEGALKKLLVGGLALIGGLTLLAFLAVGLIGFV
                     MAVAEPGVKRHTILQVDFERGVVETVPQDAFAQLMLEDQLEVLDVVAALDRAAEDNRV
                     EALVARIGGGGIGLAHLQEIRDAVLRFRESGKPAIAFSETFGEFGPGNGGYYLATAFD
                     RIYLQPSGDIGLTGLHYESMFVKGVLDNLDVEAQMDQRYEFKNAMNMYTERDLTEAHR
                     LAMAALTESQFEQMVEGIAEARGIDAPEVRRIFDEGPYLGAETVEVGLVDELRYEDEV
                     YAELEQQLDGRTNRLFLDAYLERAGGPHRRGRTVALIHAYGAVSRGQGGYSPLDGSVT
                     MGSNAVVDALRTAITSDRVEAIILRVDSPGGSYVGSDTIWRETVRAREAGKPVIVSMG
                     NLAASGGYFVAVDADRIVAQPGTITGSIGVLGGKLVTTGLWNKLGVTFDGVGSSANST
                     MFSPVHPFTEHGYRRFESWLDRVYEDFTGKVARGRELPLEEVLEVAKGRVWTGSDALE
                     IGLVDRLGGLHEAMQLAREELGLAADAPLRLRRIPADKTPLELLLGEKPDHRNAVAAA
                     AVAGIVRDLQPVVRLAREAVSSRRAGIEMPPELAVRP"
     gene            2593..>2898
                     /locus_tag="VD788_12640"
     CDS             2593..>2898
                     /locus_tag="VD788_12640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77157.1"
                     /translation="MCTMTWSEREDGYEVLFNRDERRTRKPAAPPSIRQAGSLRYLAP
                     ADGDFGGAWIAVNELGLTLAIENGYLDQDDLSREPAGGFTSRGLLVTSLAGETTVGAV
                     "
BASE COUNT          438 a         1055 c          966 g          439 t
ORIGIN      
        1 tgggtcccgc cctcgccgaa catgccgtcc gtgcggacgg cgctggtcta ccccggcggc
       61 tgtctgatcg agggcaccaa cctgtccgag ggacgcggca cgacgttgcc gttcgagttg
      121 gtcggcgcac cgtggctcga cccgcacgcg gtcgccgccg gattggattc cgagcggctc
      181 gccggcgtgc tgtcccggcc ggcgacgttc cgcccgatgt tccacaagca cgccggcatc
      241 gcctgtcacg gcgtccagct gatcgtcacg cgcgccgagg cgttcgagcc gttcctcacc
      301 tacctcgcgc tgatccgggt ggtgcgccgg ctggatcgcc gtttcgcgtg gcgctcggcg
      361 ccgtacgagt tcgagagcga acgaccggcg atcgaccacc tgctcggcgt caccggcttg
      421 cgcgagcggc tggagtcgga cgagccgctc gagcggctgc ggtccggctg ggagccggcg
      481 ctcgatcgct ttgcggagtc gcgacggcgg tacttgctct acgattgacc ccgacaaagc
      541 aaacggcgcg ggtggctcgt gccaaaccac ccgcgccgca atgtagcctt cgcgcgcgcc
      601 ggacgacaac ggggggttta tcggccagcg ggttcgatcg gctctctgtc gaaagacgag
      661 ggtaatctac cgcgaaccgc ccggcgtgga aggcgcgcgt ggtcgattcc tgcggatcgt
      721 ccgaccgcac ccccgctacg gacggacggc gagctccggc ggcatctcga ttcccgcccg
      781 gcggctcgac accgcctcgc gcgccagacg cacgaccggt tgcagatcgc gcacgatacc
      841 ggccactgcc gcggccgcca cggcgttgcg atgatcgggc ttctcgccga gcagcagctc
      901 gagcggcgtc ttgtcggccg gtatccgtcg cagccgcaac ggggcgtcgg cggccagacc
      961 gagctcctcg cgcgcgagct gcatcgcttc gtgcagcccg cccagccggt ccacgagccc
     1021 gatctcgagc gcgtcggagc cggtccacac ccgccccttg gccacctcga gcacctcctc
     1081 gagcggtagc tcgcgaccgc gggccacctt gccggtgaaa tcttcgtaca cccggtcgag
     1141 ccaggattcg aatcgccggt atccgtgctc ggtaaacggg tggaccggag agaacatggt
     1201 cgagttggca ctcgacccga cgccgtcgaa ggtcactccg agcttgttcc acagaccggt
     1261 cgtcaccagc ttgccgccca gcacgccgat cgacccggtg atcgtaccgg gctgggccac
     1321 gatccgatcg gcgtcgaccg cgacgaagta cccgcccgag gcggccaggt tgcccatcga
     1381 gacgatcacc ggcttgcccg cctcgcgggc gcggaccgtt tcgcgccaga tggtgtccga
     1441 tcccacgtac gatccgcccg ggctgtcgac tcgcaggatg atcgcctcga cgcggtcgct
     1501 cgtgatcgcg gtgcgcaacg catcgacgac cgcgttcgat cccatggtga ccgacccgtc
     1561 gagcggcgag tatccgcctt gcccgcgcga gacggcgccg taggcgtgga tcagcgccac
     1621 cgtccgaccg cgccggtgtg gaccgccggc gcgctccagg taggcgtcga ggaacagacg
     1681 gttggtccgg ccgtcgagct gctgctcgag ctcggcgtag acctcgtcct cgtagcgcag
     1741 ctcgtcgacc agaccgacct cgaccgtctc ggcgccgaga tacggaccct cgtcgaagat
     1801 ccgccggacc tccggcgcgt cgatgccgcg tgcctcggcg atgccctcca ccatctgctc
     1861 gaactgcgat tccgtcaacg ccgccatcgc cagacgatgc gcctcggtca ggtcccgctc
     1921 ggtgtacatg ttcatcgcgt tcttgaactc gtagcgctgg tccatctgcg cttcgacgtc
     1981 gaggttgtcg agcacgccct tgacgaacat gctctcgtag tgcagcccgg tcagcccgat
     2041 gtcgccggat ggctgcaggt agatccggtc gaacgcggtg gcgaggtagt agccgccgtt
     2101 gcccggaccg aactcgccga acgtctccga gaacgcgatc gccggcttgc ccgattcgcg
     2161 aaaccgcagc acggcgtcgc ggatctcctg cagatgggcg agaccgatgc cgccgccgcc
     2221 gatgcgcgcg accagcgcct cgacccggtt gtcctcggcc gcccgatcga gcgccgcgac
     2281 cacgtccagc acctcgagct gatcctcgag catcagttgg gcgaacgcat cctgcggaac
     2341 cgtctcgacc acgccgcgct cgaagtcgac ctgcaggatc gtgtgccgct tgaccccggg
     2401 ctcggcgacc gccatgacga aaccgatcag accgacggcg aggaacgcga gcagcgtcag
     2461 cccgccgatc aacgccaggc cgccaaccaa cagcttcttc agggcaccct ccgaggctcc
     2521 gccgccgggc ggggccggac cgagcatctt aacaacttcg cgtcgaagcg gcccggagcc
     2581 tagaatgccg gcgtgtgcac gatgacctgg agcgagcggg aggacggcta cgaggtcctg
     2641 ttcaaccgcg acgagcggcg cacgcgcaag ccggcggcgc cgccgtcgat ccggcaggcc
     2701 ggttcgctgc gctacctcgc cccggccgac ggcgacttcg gcggcgcctg gatcgcggtc
     2761 aacgagctcg gactgacgct ggcgatcgag aacggctacc tcgaccagga cgacctgtcg
     2821 cgcgaaccgg ccggcgggtt caccagccgc gggctgctgg tcacgtcgct ggccggcgag
     2881 acgaccgtcg gcgcggtg
//
LOCUS       DASPGN010000435        11829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_315038, whole genome shotgun sequence.
ACCESSION   DASPGN010000435 DASPGN010000000
VERSION     DASPGN010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11829
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315038"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /locus_tag="VD788_12645"
     CDS             <1..1651
                     /locus_tag="VD788_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77158.1"
                     /translation="PGRLGRLAARLMAAEADARPSLDEVERELRALGGELGNECVTPP
                     REHAAARVRLTPPPRARRIEPIEPARRQAAEARPVTRRWPVGIVAALAVVLPAAFVLV
                     LLPRWLPDRDAARPEPAAPRPLARPAEPTERQGRETVERLRARQQAERERQTVRELSD
                     ALDAKAVGRWGGVALADAARRIDTADARLRDGDYLDAATGYREATARLRELDARSTDV
                     LRRALEDGERALAAGDAAGATAAFDLALAIAPEDGTAGQGSRRAGVLDRLTELLDEGY
                     DAERAGELEVAERRYREALELDSLSRPAGEALARIESRLRDDRFAGFMSDGLAALERG
                     DYERAGQAFERARAVRPDASEVADGITRLESARKLDAIDAHRARAERHETGEAWDEAR
                     AEFDRVLELDPTIVFAQQGAVRTRARAEQAARIEFHLAHPDRLSDDDVLSDARLLLSE
                     AVEVTPAGPRHRQRVERLRELIERAATPVRVRLRSDNLTEVVVYHVGPRGSFDERELE
                     LRPGRYTVVGSRRGYRDVRHELIVPAGREPDPLWVRCEEPI"
     gene            1648..4074
                     /locus_tag="VD788_12650"
     CDS             1648..4074
                     /locus_tag="VD788_12650"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HXV77159.1"
                     /translation="MSRPVQIEAAGVRRTVGPEAFPLSCGATECDLALDVVGAGGPVA
                     WLGQTDGELFVEVARDDVPISCNGVPVTSSQWLRDGDRLRVGNSVIRLSAAGGELGLV
                     VETTEDDVTEPPALLPAEFVGSDAESDRTIRPIAYRPGATGDRAPAVRRRGRAAWAAW
                     IAVALAAGAAWFVFATRSVEIRVEPPPERLRLSGTPPVIRLAGRYLVRPGDHRVVAEK
                     PGYEPLDVTIEVTGESNQTFEFRLSPLPDLVAITTGGVDGARVWVDGIEIGVTPLSPF
                     PLAAGEHELRVEAERHAAHLQPLRVDGGGDSVPVEVQLVPRFATVRFRSEPAGATVQV
                     DGVVIGETPLTADVDEGRRRVRFLLTGHQPFDDTLRIDAGAAIDLPLVRLAPAQAALA
                     LDSQPPGAIVTLDGDYAGETPIELAIEPTRASRIGLSKAGYETAWRTLSLRSGERAAI
                     EVDLVPRYGEVAVRAVPPDAELWVDGTARGRADQTLRLVAVPQRIEIRKPGFLDFRTE
                     VTPRPDFPQVVHAILKTEEQIAAERRPPRIRTSEGQELLLIEGGRFRMGAPRREPGRR
                     ANETERDVELTRPFYLSTTEISNRDFRRFRAEHLSGQVGGHNLEIDNHPVVRVSWNDA
                     ALFCNWLSERDSLDPFYEAAGGRVVAVVPTTNGYRLPTEAEWAWAARFDADGRERKYP
                     WGDALPIPAAAGNFADRSAAGMLSTTLDAYDDGYPATAPVDGFAPDVRGLFQVGGNVA
                     EWIHDGYTVNSPAAEPVVDPIGPDAGELHVIRGSSWMDATISELRLTFRDYGTGGRPD
                     LGFRIARYAD"
     gene            4094..4282
                     /locus_tag="VD788_12655"
     CDS             4094..4282
                     /locus_tag="VD788_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77160.1"
                     /translation="MRGPLVLLLLVLLSTTPQGLRSAASADAPEPSAAPATDESLEEF
                     VPSEEISADNAVSFPVDI"
     gene            4300..5100
                     /locus_tag="VD788_12660"
     CDS             4300..5100
                     /locus_tag="VD788_12660"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV77161.1"
                     /translation="MKRSFPDEFLFQLASLVLALAVVHGFYVLLVRPNAETILAEQTA
                     RMQIEPGYVPPRSAWVVVRDYEQEACFVLMLWAFAIMGHKSFVTGRERRLLEADLIPL
                     GEGTKILPEDAREYSRQIQSLPPADQRRLVPRSLLTALHRFTATRSIQDVTSTAHGVC
                     EAESARLESELTMVRYIAWAIPSIGFIGTVRGIGDALGQAHKAVQGEIAGVTQSLGTA
                     FNSTFIALLISIVLMFLLHQLQLQQERLVLDTEHYLDQHLIQHLQVPA"
     gene            5109..6518
                     /locus_tag="VD788_12665"
     CDS             5109..6518
                     /locus_tag="VD788_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXV77162.1"
                     /translation="MKTLALELNDAGVIALADAGPAAERPIVSPGLAIVDGRDVATGH
                     RALERARLNPRKLHSRFWTRPDAGPLGRPFGDEWTHADLVHEHFRALWQSAGDGIEQV
                     VLAVPGSYSEPQLGLLLGIAGAAGIPVTGLVDAAVAAATRVAPVPWLVHLDLRLHDVV
                     LTELAVSDGEWIRGAVRTSDMTGLAVLRDTWAKYVAAQFVRRTRFDPLHSATTEQELY
                     LALDGLVDELRTNERASITLQRAGRAFSLELTRGELAATTASTCDALVDWVRRARSAA
                     ELASVSISHHAAAVPSLVDGLADLDEAEPIVLGQTAAAEGALVHRREICAPGDGLPYV
                     TRLPAVAPAGAAAAPHGRARTRPASSAAGVPTHVLWEDRAYEITGEPLEFGSAPRFAR
                     SVRIVGSTAGLSRRHCVFLRNGSSVLVRDHSTHGTFVNGRRVEHEARVGPGDRVRIGS
                     PGVELRLIRVVGRETPPAD"
     gene            6496..7590
                     /locus_tag="VD788_12670"
     CDS             6496..7590
                     /locus_tag="VD788_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77163.1"
                     /translation="MRRRRQIEVFGLSFLDAITCGFGAVVLFYMVINAAVGMRAGRMT
                     GELRAEADRLEVEVLAAYQDLVDLRNRTRRLDDDSAVAFGLARRLIEQVEAIRHELAT
                     YDETTLAREQQLNKLKADLRALEQEARRLSAAAPSDETPGDELRAFIGDGERQYLTGL
                     KVGGQRIVILVDASASMLDETIVNVIRRRNLPDETKIRADKWQRAVRSVDWLTTQIPA
                     DSQFQLYTFNSRLTAVLPDSAGRWLDGGSRDDLDRAVAALRGVVPRDGTNLEAGLAVL
                     DQLRPAPDNLILLVDGLPTLGRGSSERGMVSGKQRMRLFERAVAKLSPRLPVNVILLP
                     MDGDPMAASAFWRLAVTSGGSLINPAEDWP"
     gene            7587..8600
                     /locus_tag="VD788_12675"
     CDS             7587..8600
                     /locus_tag="VD788_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77164.1"
                     /translation="MKRRSRAQGEGFSLSFLDCICCGFGAIVLLLVLTKIGEPAALER
                     AREELDRRVARLQLELEQIRGETRVLERESLAKQEQLSVERELIARLSGDLSAIQGEF
                     AASRQQSEVHDIVEGRLLAARQQLTEEMTRLAAQQPRRPSPADRPVGGIPVDSEYVIF
                     VIDTSGSMQGYTWELVVEKMAQTLDIYPNVKGMQVMNDMGQYMFPQYAGKWIPDTPAR
                     RSAVLSRLRSWQAFSNSSPVEGIVRAIQAFAAADKKVSLYVFGDEFTGPSIGDVLETV
                     ERINTRDAEGQPRVRIHGIGFPVMFTTPGVRDFTGVRFATLMRELCRENGGTFVGLNH
                     VDP"
     gene            8752..10308
                     /locus_tag="VD788_12680"
     CDS             8752..10308
                     /locus_tag="VD788_12680"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77165.1"
                     /translation="MRSREPMTVAAVDLGSNSFHLIIAHFVDGELQIIDRMREQVMLA
                     DGLDDRSLLAEEAQQRALACLERIGQRLRSLPSVRVRAVGTNTLRRAKNADAFRARAQ
                     QSLGHPIEIVSGQEEARLIYLGVAHDRFSSAPRLLADIGGGSTEIIVGEGYRPVRSHS
                     LFMGCVNYSKRFFPDGGLRKDAFREAEIAAALELRTIGREIKRMGWDDCLGSSGTIIT
                     IQEILRATGWSDGAITWPGLKRLRRSLVDAGAIERIGLPGVKAGRARVLPGGLAILRA
                     IFRSLEIESMAAASGALREGVLHDLVGRIRHEDIRDHTIQRIVERYRVDVGQAMRVEH
                     VALRLLTEFERPWQIDSERLRKFVIWAAQLHEIGLGISHTGFHKHGAYLIEHSDMPGF
                     SADDQQILAALVRSQRRKLSKPVFSRLTEGGGEAIVRLCIVFRLAVLLNRSRSPQVVP
                     AVRVGDRWSRLALQFPAGWSEAHPLTRADLLEEAGYLRGVGVEMTVEEEPARGEDSSG
                     PNGSVEGSAR"
     gene            10305..10808
                     /locus_tag="VD788_12685"
     CDS             10305..10808
                     /locus_tag="VD788_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXV77166.1"
                     /translation="MKRLLVLRHAKSSWRDQALDDHERPLNKRGRRDAPRIGLLMERR
                     GLLPQIAITSSALRARATLELALEPREWAGEIRVSPRVYGASATEIVDVLGETPGRYD
                     RLLLVGHNPTLEELVAGLTGRAVALPTGALAVIDGAWSDWGALALDGAAELVALYTPR
                     RLDDEDG"
     gene            complement(10805..11350)
                     /locus_tag="VD788_12690"
     CDS             complement(10805..11350)
                     /locus_tag="VD788_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77167.1"
                     /translation="MRLSRSFPATAVLLLACCLPCPTSSAAEPTETEAVGPNVRITLT
                     VGDVDPGAQPVVRTYRLLTRDGGEPSRMLMGWRTPIPMSKGAGDELSYVYQNVGMTAQ
                     IRTTVRAGGTVLVSGEVEISGARQAIEGMQIPPHLPVIGTFQQDLSVSLEPGKPLRVA
                     EVPDPEGGTMYLELTAEVLDR"
     gene            11456..>11829
                     /locus_tag="VD788_12695"
     CDS             11456..>11829
                     /locus_tag="VD788_12695"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXV77168.1"
                     /translation="MEFSERQTRIITTAATVLALVIILAASAALTWLVTLFLRSFSHV
                     LLPPAVAAVIALVVEPYYRLLNRRLGLPMPLALLTLMLSALLPAIAFAWFFGALLVDQ
                     LAQLVEQFPAWWETTRQRVEQR"
BASE COUNT         1702 a         4066 c         4250 g         1811 t
ORIGIN      
        1 gcccggtcgc ctcggccggc tcgccgcccg gctgatggcc gccgaggccg acgctcggcc
       61 gagcctcgac gaggtcgaac gggagcttcg cgcgctcggc ggggagctcg gcaacgagtg
      121 cgtcacgccc ccgcgcgagc acgccgccgc cagggtcagg ctcaccccac ctccgcgcgc
      181 ccgtcggatc gagccgatcg agccggcgcg gcggcaggct gcggaggctc gaccggtcac
      241 ccgacggtgg cccgtgggaa tcgtcgcggc actcgccgtc gtcctgcccg cggcgttcgt
      301 gctcgtactt ctgccgcgct ggcttcccga ccgggacgcc gcgagaccgg agcccgccgc
      361 tccgcgaccg ctcgcgcgac cggccgaacc gaccgagcgc cagggtcggg agacggtcga
      421 acggctgcga gcgcggcagc aggccgagcg cgagcgccaa acggtgcgcg aactgtccga
      481 cgccctcgac gccaaggccg tcgggcgctg gggcggcgtg gccctggcgg acgccgcgcg
      541 tcggatcgac acggccgatg cccggctgcg agacggagac tatctcgacg cggcgacggg
      601 ctatcgcgaa gcgaccgcgc gcctgcgcga gctcgatgca cgatccacgg acgtcctgcg
      661 ccgggcgctc gaggacgggg agcgggcgct cgcggcgggg gacgccgcgg gggcgaccgc
      721 cgccttcgac ctggccctcg ccatcgcgcc cgaggacgga acggcggggc aggggagccg
      781 tcgcgccggc gtgctcgatc gactgaccga gctgctcgac gaagggtacg acgccgagcg
      841 cgccggggag ctcgaggtcg cggagcgacg ctaccgagag gcgctcgagc tcgattcgct
      901 gtcgcggccg gccggcgagg ctctggcgcg catcgaatca cgcctgcgcg acgaccggtt
      961 cgccggtttc atgtccgacg gactcgcggc gctcgagcgt ggagactacg aacgggcggg
     1021 ccaggcgttc gagcgggcgc gtgccgtccg tcccgacgcg agtgaggtgg cggacggaat
     1081 cacgcggctc gagtcggccc gcaagctcga cgcgatcgac gcgcaccgcg cgcgggccga
     1141 acgccacgaa accggcgagg cgtgggacga agcgcgcgcc gagttcgatc gcgtgctcga
     1201 gctcgatccg acgatcgtct tcgcacagca aggcgcggtg cgcacgcgcg ctcgcgccga
     1261 gcaggccgct cgcatcgagt tccacctcgc gcaccccgac cggttatccg acgatgacgt
     1321 cctgtccgac gcccggctgc tgttgagcga ggccgtcgag gtgacgccgg ccggcccgcg
     1381 ccaccggcag cgcgtcgaac ggctcaggga gctgatcgag cgcgcggcga ctccggtgcg
     1441 ggtgagattg cgctcggaca atctgaccga ggtcgtcgtc taccacgtcg gcccgcgggg
     1501 gagcttcgac gagcgagagc tcgagctgcg cccggggcgt tacaccgtgg tcggcagtcg
     1561 gcgcgggtat cgcgacgtgc gccacgagct gatcgtgccc gcgggccggg agcccgatcc
     1621 gctatgggtc cgctgcgagg agccgatatg agccgtccgg tccagatcga ggccgcgggt
     1681 gtccggcgga ccgtgggacc ggaggcattc ccgttgtcgt gcggcgcgac ggagtgcgat
     1741 ctggcgctcg acgtcgtcgg cgccggcggg ccggtggctt ggctcggcca gaccgacggg
     1801 gagttgttcg tcgaggtcgc gcgcgacgac gtgccgatca gctgcaacgg cgtgccggtg
     1861 acgagttcgc agtggctccg tgacggcgac agactgcgcg tcggcaacag cgtgatccga
     1921 ttgtcggcgg ccggcggcga gctggggctc gtcgtcgaga cgaccgagga cgacgtgacg
     1981 gagccgccgg cgctcctgcc cgcggagttc gtcggctccg acgcggagtc cgaccggacc
     2041 atccgaccga tcgcctaccg gcccggggcg acgggagatc gcgcaccggc agtccgtcgc
     2101 cgaggtcgtg cggcgtgggc tgcgtggatc gccgtcgcgc tcgccgccgg ggccgcctgg
     2161 ttcgtcttcg ccacgaggtc ggtcgagatc cgcgtcgaac cgccgcccga gcggctgcgg
     2221 ttgtccggca cgccgcccgt gatccgcttg gccggccgct acctcgtccg accgggtgac
     2281 catcgtgtgg tcgcggaaaa gccgggctac gagccgctcg acgtcacgat cgaggtgacc
     2341 ggagaatcga accagacgtt cgagttccgc ttgagtccgc tgcccgatct cgtcgcgatc
     2401 acgaccggcg gggtggacgg cgcccgcgtg tgggtcgacg gcatcgagat cggcgtcact
     2461 ccgctttcgc cgttcccgct cgccgcgggc gagcacgagc tgcgtgtcga ggccgagcgt
     2521 cacgcggctc acctgcaacc gctccgcgtc gacggcggcg gtgacagcgt cccggtcgag
     2581 gtgcagctcg tgccacggtt cgcgacggtc cgcttccggt cggaaccggc gggcgcgacg
     2641 gtgcaggtcg acggcgtggt catcggcgaa acgccgctga ccgccgacgt cgacgagggg
     2701 cgccgccgcg ttcgattcct gctcacgggt catcagccgt tcgacgacac gctccgcatc
     2761 gacgccggcg ccgcgatcga tctgccgctc gtccgacttg cgccggcgca ggccgccctc
     2821 gcgctcgaca gccaaccccc gggcgcgatc gtcacgttgg acggcgacta cgcgggcgag
     2881 acgccgatcg agctcgccat cgaaccgacg cgcgccagcc ggatcggcct gtccaaggcc
     2941 ggctacgaga ccgcctggcg cacgctctcg ctgcgctcgg gggagcgggc ggcgatcgag
     3001 gtcgatctcg tgccgcgcta cggcgaggtg gccgtccgcg ccgtaccgcc ggacgcggag
     3061 ctctgggtcg acggcaccgc ccggggacgc gcggaccaga cgctgcggtt ggtcgcggtg
     3121 ccccagcgca tcgagatccg caagccgggc tttctcgact ttcgcaccga ggtaacaccg
     3181 cggcccgact ttccgcaggt cgttcacgct atcctgaaga ccgaggagca gatcgcggcc
     3241 gagcgccggc cgccgcggat ccggacgtcc gagggacagg agctcttgtt gatcgaaggg
     3301 ggacgattcc gcatgggcgc gccgcgccgc gaacccggtc gtcgggcgaa cgagacggag
     3361 cgcgacgtcg agctgacgcg tccgttctat ctgtcgacaa ccgagatcag caatcgcgat
     3421 ttccgccgct ttcgggccga gcacctatcg ggtcaggtcg gtggtcacaa tctcgagatc
     3481 gacaaccacc ccgtggtccg ggtgagctgg aacgacgcgg cgctgttctg caactggctc
     3541 agcgagcggg actcgctcga tccgttctac gaggcggccg gcggccgcgt cgtcgcggtc
     3601 gtgccaacga ccaacggcta ccgactgccg accgaggccg agtgggcctg ggccgcgaga
     3661 ttcgacgccg acggccggga aaggaagtac ccgtggggcg acgcgctgcc gattcccgcg
     3721 gcggcgggaa atttcgccga ccggtcggcg gcgggcatgc tgtcgacgac gctcgatgcc
     3781 tacgacgacg gctacccggc gaccgccccg gtcgacggct tcgcgccgga cgtgcgtggc
     3841 ctgttccagg tcggcggcaa cgtcgccgag tggatccacg acggctacac cgtcaactcg
     3901 cccgcggccg agccggtggt cgatccgatc ggccccgatg ccggcgagct acacgtgatc
     3961 cgcggctcga gctggatgga cgcgacgatt tccgagctac gattgacctt ccgcgactac
     4021 ggcacgggcg ggcgccccga cctcgggttt cgcatcgccc gttacgcgga ttgagcgcgg
     4081 acgggagacg ccgatgagag gtccgctggt gttgctgctg ctggtcctgc tttcgaccac
     4141 cccgcaaggt cttcgctccg cggcatccgc ggacgcgccg gagccgtcgg ccgcacccgc
     4201 gaccgacgag tcgctcgagg agttcgttcc gtccgaggag atctcggccg acaacgccgt
     4261 ctcgttcccg gtcgacatct gatcggtctt cgggtcgtga tgaagagatc gttccccgac
     4321 gagttcctgt tccaactcgc gtcgctcgtc ctcgcgctgg cggtcgtgca cgggttctac
     4381 gtgctgctcg tccgccccaa cgcggagacg attctcgccg agcaaaccgc acgcatgcag
     4441 atcgagccgg gctacgtgcc gccgcgctcg gcgtgggtcg tcgtccgcga ctacgaacag
     4501 gaagcctgtt tcgtgctgat gctgtgggcg ttcgccatca tgggccacaa gagcttcgtc
     4561 accgggcgcg agcggagact gctcgaggcc gacctgatcc cgctcggcga ggggacgaag
     4621 atcctgccgg aggatgcgcg cgagtactcg cgccagatcc agtcgctacc acccgccgac
     4681 cagcgtcgtc tggtcccgcg ctcgctgctg acggcgctgc accgcttcac cgcgacgcgc
     4741 agcatccagg acgtcacgag caccgcccac ggcgtgtgcg aggccgaatc ggcgcgactg
     4801 gagtccgagc tgacgatggt ccgatacatc gcctgggcca tcccgtcgat cggcttcatc
     4861 ggcaccgtgc gcgggatcgg tgatgcgctc ggtcaagcac acaaggcggt ccagggcgag
     4921 atcgcggggg tcacgcagag cctgggcacc gccttcaact cgacgttcat cgcgctgctg
     4981 atcagcatcg tgctgatgtt cctgttgcac cagctgcaac tgcaacagga gcggctggtg
     5041 ctcgacaccg aacactacct cgaccaacac ctgatccaac atctgcaggt tccggcctga
     5101 acgcgaccgt gaagacgctc gccctcgaac tcaacgacgc cggcgtgatc gccctcgccg
     5161 atgccggacc ggcggccgaa cggccgatcg tcagcccggg cctggcgatc gtcgacgggc
     5221 gcgatgtcgc gacgggccac cgcgcgctcg agcgtgcgcg attgaatccg cgcaagctcc
     5281 acagccggtt ctggacgcgc cccgacgccg gcccgctcgg gcggccgttc ggcgacgaat
     5341 ggacccacgc ggacttggtc cacgagcatt ttcgcgcgct gtggcagagt gccggcgacg
     5401 ggatcgagca ggtggtgctg gcggtccccg gcagctatag cgagccgcaa ctcggcctgt
     5461 tgctcgggat cgccggcgcc gcgggcattc cggtgaccgg tctggtcgac gcggcggtcg
     5521 ccgccgcgac gcgggtggct ccggtgccgt ggctcgtcca cctcgacctc cgcctacacg
     5581 acgtcgtgct gaccgagctc gcggtgtcgg acggggagtg gatccgcggt gcggtgcgca
     5641 cgagcgacat gacggggctg gccgtcctac gcgacacctg ggccaagtac gtcgccgccc
     5701 agttcgtccg acgcacccgt ttcgacccgt tgcactcggc gacaaccgag caggagctct
     5761 acctcgcgct cgacgggctg gtcgacgagc tgcgaacgaa cgagcgcgcc tcgatcaccc
     5821 tgcaacgcgc cgggcgggcc ttctccctcg agctgacccg tggggagctc gcggcgacga
     5881 cggcgtcgac ctgcgacgcg ctggtcgact gggtgcggcg cgcccgttcg gctgctgagc
     5941 tggcgtccgt ctcgatctca caccacgccg ccgccgtacc atcactcgtc gacgggctgg
     6001 ccgacctgga cgaggcggag ccgatcgtcc tcggtcagac cgccgccgcc gaaggcgcgt
     6061 tggtccatcg gcgcgagatc tgtgctccgg gcgacgggtt gccctacgtc acccggttgc
     6121 cggcggtcgc cccggccggc gctgccgccg cgccgcacgg tcgagcgcga acgcgcccgg
     6181 cgagctcggc cgccggcgtg ccgacccacg tgttgtggga ggaccgggcc tacgagatca
     6241 ccggcgagcc gctcgagttc ggttcggcac cacgcttcgc gcggtcggtc cgcatcgtcg
     6301 ggtcgacggc cggtctctct cgtcggcatt gcgtcttcct gcggaacggc tcgtccgtgc
     6361 tggtccgtga ccacagcacc cacgggacct tcgtcaacgg ccggcgcgtg gagcacgagg
     6421 cccgggtggg gccgggcgac cgggttcgca tcggctcgcc cggcgtcgag ctccgtttga
     6481 tccgggtggt gggtcgtgag acgccgccgg cagattgagg ttttcggtct ctccttcctc
     6541 gacgcgatca cctgcgggtt cggcgcggtc gtgctgttct acatggtgat caacgccgcg
     6601 gtcggcatgc gcgccggtcg catgaccggc gagctgcgcg cggaggccga ccggttggaa
     6661 gtcgaagtgc tggcggccta ccaggacctc gtcgatttgc gcaatcggac gcggaggctg
     6721 gacgacgaca gcgccgtcgc cttcggtctg gcacgccggt tgatcgagca ggtcgaggcg
     6781 attcggcacg agctcgcgac ctacgacgag acgacgctcg cgcgcgagca acagctgaac
     6841 aagctgaagg ccgatctcag ggcgctcgag caggaggcac ggcgcctgtc ggccgccgcg
     6901 ccgtccgacg agacgccggg cgacgagctg cgcgcgttca tcggcgacgg cgagcgacag
     6961 tacctcaccg ggctgaaggt cggcgggcag cgcatcgtca tcctggtcga cgcctcggcc
     7021 agcatgctcg acgagacgat cgtcaacgtc atccgtcgcc ggaacctgcc cgacgagacg
     7081 aagatccgcg ccgacaagtg gcaacgcgcc gtccgctcgg tcgattggtt gacgacacag
     7141 atcccggcgg attcgcagtt ccagctctac acgttcaact cgcgattgac cgctgtgctg
     7201 ccggattccg ccggtcgctg gctcgacggc ggcagccgcg acgatctcga tcgcgcggtc
     7261 gccgcgctgc ggggggtcgt gccgcgcgac gggaccaacc tggaagccgg cttggcggtg
     7321 ctcgatcaac tgcggccggc gcccgacaac ctgatcctgc tggtcgacgg gctgccgacg
     7381 ctcggtcgcg ggtcgtccga gcgggggatg gtcagcggca agcagcgaat gcgtctgttc
     7441 gaacgtgcgg tggcgaagct cagcccgcgc ctaccggtca acgtcatcct gttgccgatg
     7501 gacggtgatc cgatggcggc cagcgcgttc tggcgcctgg ccgtcacgag cggcggctcg
     7561 ttgatcaacc cggccgagga ctggccgtga agcgccgctc gcgcgcgcag ggggagggct
     7621 tcagcctgtc gttcctcgac tgtatctgtt gcgggttcgg tgcgatcgtg ctgctgctcg
     7681 tgctgaccaa gatcggcgag ccggcagcgc tcgagcgggc gcgcgaggag ctcgatcggc
     7741 gcgtggctcg gctgcagctc gagctcgagc agattcgcgg ggaaacgcgg gtgctcgagc
     7801 gcgaatcgct cgccaagcag gagcagctgt cggtcgagcg cgagctgatc gcgcggctgt
     7861 ccggcgacct gtcggcgatt cagggcgagt tcgcggcgag ccgccaacag tcggaggtgc
     7921 acgacatcgt cgagggccga ttgctcgccg cccgtcagca gttgacggaa gagatgaccc
     7981 ggctggcggc gcagcagccg cgtcgtccgt cgcccgccga tcggccggtc ggcggcattc
     8041 cggtcgacag cgagtacgtg atcttcgtca tcgacacgtc gggcagcatg cagggctaca
     8101 cctgggagct ggtggtggag aagatggcgc agacgctcga catctatccc aacgtcaaag
     8161 gtatgcaggt gatgaacgac atgggccagt acatgtttcc gcagtacgcc gggaagtgga
     8221 tacccgatac ccccgcgcgg cgaagcgcgg tgctgagccg cctgcgcagt tggcaggcgt
     8281 tcagcaactc gagcccggtc gagggaatcg tccgtgcgat ccaggccttc gccgcggcgg
     8341 acaagaaggt cagcctctac gtcttcggcg acgagttcac cggaccgtcg atcggcgacg
     8401 tgctggagac ggtcgaacgg atcaacacgc gcgacgcgga gggccaaccg cgcgtgcgga
     8461 tccacggcat cggcttcccg gtgatgttca ccacacccgg agtccgcgat ttcaccggag
     8521 ttcggttcgc cacgctgatg cgcgagctgt gccgcgagaa cggcggcacc ttcgtcggcc
     8581 tgaatcacgt cgacccatga gtctcggcgc gggcgtcggc cgtcgccggt cgaccggttt
     8641 accgccggcg gcgacgggga gtagtatttc ggctcggctg ctcgacggcc tggggacgcg
     8701 cggcaacccg ccgcgcgcct gaggcgaccg gcagcgcagg agagaggtcc ggtgagatcg
     8761 cgcgagccga tgacggtggc cgccgtcgac ctggggtcga acagcttcca tctgatcatc
     8821 gcgcacttcg tcgacggcga gctgcagatc atcgatcgaa tgcgcgagca ggtgatgctg
     8881 gccgacgggc tggacgatcg atcgttgctc gctgaagaag ctcagcagcg ggcgctcgcc
     8941 tgtctcgagc ggatcggcca gcgcctgcgc agcctgccct cggtacgagt tcgtgcggtc
     9001 ggcaccaaca cgctcaggcg ggcgaagaac gccgatgcct ttcgcgcgcg ggcgcagcag
     9061 tcgctcggcc atccgatcga gatcgtgtcg gggcaggaag aggcgcggct gatctacctc
     9121 ggcgtggcgc acgaccgctt ctcgagcgcc ccgcgtctgt tggccgacat cggcggtggc
     9181 agcaccgaga tcatcgtcgg tgagggctat cggccggtcc gctcgcacag cctgttcatg
     9241 ggctgcgtca attattccaa gcgattcttc cccgacgggg gcttgcgcaa ggacgcattc
     9301 cgcgaggccg agatcgccgc cgcgctcgag ctgcgcacga tcggtcgcga gatcaagcgg
     9361 atgggatggg acgactgcct cggctcgtcc gggacgatca tcacgattca ggagatcctg
     9421 cgtgcgacgg gctggagcga cggtgcgatc acctggcccg gcctcaagcg gctgcgtcgg
     9481 tcgctggtcg acgccggcgc gatcgagcgg atcgggctgc cgggggtcaa ggccggtcgc
     9541 gcgcgtgtgc tcccgggcgg tctggcgatc ctgcgcgcga ttttccgcag cctggagatc
     9601 gagtcgatgg cggcggccag cggcgcgctg cgcgagggcg tgctgcacga cctcgtcgga
     9661 cggattcgcc acgaggacat tcgagaccac acgatccaac gcatcgtcga gcggtaccgc
     9721 gtcgatgtcg ggcaagccat gcgcgtcgag catgtcgcgc tgcgcctgct gacggagttc
     9781 gaacggccct ggcagatcga ctcggagcgc ctgcgcaagt tcgtcatctg ggccgcgcag
     9841 ctgcacgaga tcggtctcgg catctcgcac accgggttcc acaagcacgg cgcctacctg
     9901 atcgagcaca gcgacatgcc ggggttctcc gccgacgacc agcagatcct ggcggcgttg
     9961 gtacggtccc agcggcgcaa gctgtcgaag cccgtgttca gccgcctgac cgaaggcggc
    10021 ggcgaggcga tcgtccgtct ctgcatcgtc ttccgtctcg ccgtcttgct gaatcgcagc
    10081 cgctcgccac aggtggtgcc ggcggtacgg gtcggagacc gctggtcgcg gctggcgctc
    10141 cagttcccgg ccggatggtc cgaagcacac ccgctgacgc gtgccgatct gctcgaggag
    10201 gccggctacc tgcgcggcgt cggcgtcgag atgacggtcg aggaagaacc ggcgcggggc
    10261 gaggattcgt cggggccgaa cggctccgtc gagggttccg cgcgatgaag cgcctgctcg
    10321 tcttgcgcca cgcgaagtcg agctggcgcg atcaggcgct cgacgaccac gagcggccgt
    10381 tgaacaagcg gggccggcgt gatgctccac gcatcggcct gctgatggag cgacgcggtt
    10441 tgctgccgca gatcgccatc acctcgtcgg cgttgcgggc ccgagctacg ctcgagctcg
    10501 cgctcgagcc tcgcgagtgg gcgggggaga tccgcgtctc ccctcgggtc tacggcgcca
    10561 gtgcgaccga gatcgtcgac gtgctgggtg agacgccagg gcggtacgac cggctgctgc
    10621 tcgtcggtca caatccgacg ctcgaggagt tggtcgccgg gttgaccggc cgcgcggtcg
    10681 ctctcccgac cggcgcgctc gcggtgatcg acggggcgtg gagcgattgg ggggcgctgg
    10741 cgctcgacgg ggcggccgag ctcgtcgccc tctacacgcc gcgacggctc gacgacgaag
    10801 acggctagcg gtcgagcact tccgcggtca actcgaggta catcgtcccg ccttccgggt
    10861 cggggacctc ggccacgcgc aacggcttgc ccggttcgag tgagacgctc aggtcttgtt
    10921 ggaaggtgcc gatgaccggc aggtggggag gaatctgcat cccctcgatc gcctggcgtg
    10981 cgccgctgat ctcgacctcc cccgacacca acaccgtccc gccggctcgt acggtcgtcc
    11041 gaatctgggc ggtcatgccc acgttctggt agacgtagga cagctcgtcg ccggcgccct
    11101 tcgacatcgg gatcggtgtc ctccaaccca tcagcatgcg cgacggctcg ccgccgtccc
    11161 gcgtcaacag ccggtaggtg cgaacgaccg gttgcgctcc cggatcgacg tccccgacgg
    11221 tcaacgtgat ccgcacgttg gggccgacgg cctccgtctc ggtcggctcc gcggcggacg
    11281 aggtcgggca gggcaggcag caggccagca acaggacggc ggtagccggg aacgatctcg
    11341 acagcctcac ggttcacctc cacggctcgg gcttcggcca ccgccactgt actacaccgg
    11401 aacggctgcc ggcttgaccc gggccgggac tttccccgat gcttgggccc gcgttatgga
    11461 gttcagcgaa cggcagaccc gcatcatcac caccgccgcc acggtgttgg cgctggtcat
    11521 catcttggcg gcgagcgccg cgctcacttg gctcgtcacg ctgttcctgc ggtccttctc
    11581 gcacgtgctg ctgccgccgg ccgtggcggc ggtcatcgcg ctcgtcgtcg agccgtacta
    11641 ccgcttgctc aaccgacggt tggggctgcc gatgccgctg gccttgctca cgctgatgct
    11701 gtcggcgttg ttgcccgcca tcgcgttcgc ctggttcttc ggcgcgttgc tcgtcgatca
    11761 actcgcgcag ctcgtcgagc agtttcccgc gtggtgggag acgacgcgtc aacgagtcga
    11821 gcagcgttg
//
LOCUS       DASPGN010000436         3439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_899617, whole genome shotgun sequence.
ACCESSION   DASPGN010000436 DASPGN010000000
VERSION     DASPGN010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899617"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VD788_12700"
     CDS             <1..399
                     /locus_tag="VD788_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77169.1"
                     /translation="GSVDYAAQPVAAYLDRPIYYPESESFGTFIDWGPARRHVPHSEV
                     IRQAAALFDRRGREIVLILNYRPGGVTLDRTVAVGAGLRMRCFAELTGAIVADENYHL
                     CRLWRPGDRHRGVDSDAAATDNDRGLENER"
     gene            423..1367
                     /locus_tag="VD788_12705"
     CDS             423..1367
                     /locus_tag="VD788_12705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXV77170.1"
                     /translation="MTPPELSFVIPVYNGAGSVGAVVERIHELYPGIELEVVLVNDGS
                     RDASERVCAELARRFPDTVTFVHLARNFGEHGAVLAGLHETRGRYVAVLDDDGQNPPE
                     EVRRLYEAITGEGYDVVYGRYRVKHHSWLRNLGSRFNDRVANVMLGKPPGLYLSSFKI
                     MNRFLVDEITRYKGAFPYIDGLVLRATGNLGQIDVDHRERDGESTYTFRKLVHLWLNM
                     FLNFSITPLRVSALLGMATSLVSLFLLVAIVVDKLYAKAEVTAGIPTVLVVVVFFAGI
                     QLVILGTIGEYLGRLFLDHSNAPQFVVRYVKRGGARDG"
     gene            1360..2349
                     /locus_tag="VD788_12710"
     CDS             1360..2349
                     /locus_tag="VD788_12710"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXV77171.1"
                     /translation="MDEFYRGKRVLITGGVGFIGSNLAIRLVGAGARVQLVDSMLPAY
                     GADLASLEPVRDRLRVNFSDVRDQHSLSYIVRGEDVIFSLAGQVSHSDSMHDPLTDLE
                     INCRSQLSLLECCRQGNPDATIVFASTRQLYGRPRYLPVDEGHPLKPVDVNGINKLAA
                     EMYYSLYHEVHGIGTVSLRMTNTYGPRMDITTGRKGFVGVFIDRALRGETIRVFGSGE
                     QRRDFNYVDDALDALLMAGERPELRGGVYNLGHPKTYSLIEFVETLARVHPLEFECVP
                     FPPEAAAIDIGDYQGDFTRFERATGWRPIVDLEEGLRRTVAYFRGRGGPHDAR"
     gene            2346..>3439
                     /locus_tag="VD788_12715"
     CDS             2346..>3439
                     /locus_tag="VD788_12715"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXV77172.1"
                     /translation="MIPFFDYRPQYARLRSGIDDAIQRVLDSGQLILGSEVDAFESEF
                     ASFVGVGGAVGVNSGTDALIVALRALGIGAGDEVITVANAGTPVVAAIRATGAMPRFV
                     DVDPVSLQLAPERLGDALSERTRCVIPVHLYGQPAPIETIAAFAEQHELALVEDCAQA
                     HGARVGGRHVGTFGRVGCFSFYPTKNLGAFGDGGICISDDPELLERLRLERMYGYRGD
                     RSSQIEGINTRLDEIQAAILRVKLSRLDGALDERRRLARRYLDGLATTSLTLPAPPPT
                     VEHAFHLFVVRHDERDRVGEQLSRCRIGWAVHYPQPVHRMPAYRFLAYDEGRLPVTEQ
                     ASRSVLSLPLYPGLEDQAVDRVIEALTSLA"
BASE COUNT          528 a         1178 c         1151 g          582 t
ORIGIN      
        1 ggcagcgtcg attacgccgc gcagccggtc gccgcctacc tcgaccggcc gatctactac
       61 ccggagagcg agtcgttcgg caccttcatc gactggggac cggcccggcg gcacgttccc
      121 cactcggagg tgatccgtca ggccgcggcg ttgttcgacc ggcgcgggcg cgagatcgtg
      181 ctgatcctca attatcggcc gggcggcgtc acgctcgatc gcacggtcgc ggtcggtgcc
      241 ggcttgcgca tgcgctgctt cgccgagttg acgggggcga tcgtggccga cgagaactac
      301 cacctctgtc ggttgtggcg tcccggcgat cgacaccgcg gcgttgacag cgacgcggcg
      361 gcgaccgata atgaccgggg actcgagaac gagcgataac ttcttatttt tgttgcagat
      421 aagtgacacc ccccgaactg tccttcgtca tcccggtcta caacggggcc ggctcggtcg
      481 gcgccgtcgt cgaacggatc cacgagctgt atcccggcat cgagctcgag gtggtgctgg
      541 tcaacgacgg atcgcgggac gcgtcggaac gggtctgcgc cgagctggca cgtcggttcc
      601 ccgacacggt caccttcgtc cacctggcgc gtaacttcgg cgagcacggg gcggtgctgg
      661 ccgggttgca cgagacgcgc ggacgctacg tcgcggtgct cgacgacgac gggcagaacc
      721 cgcccgagga ggttcggcgt ctgtacgagg cgatcaccgg cgagggatac gacgtggtct
      781 acgggcgtta ccgggtcaag caccactcct ggctgcgcaa tctggggagc cggttcaacg
      841 accgcgtggc caacgtgatg ctcggcaagc ctccggggct ctacctgtcg agcttcaaga
      901 tcatgaaccg gttcctcgtt gacgagatca cgcgctacaa gggcgcgttt ccctacatcg
      961 acggcctggt cctgcgcgcg accggcaacc tgggccagat cgacgtcgat catcgcgagc
     1021 gcgacgggga gtcgacctac accttccgca agctggtcca cctgtggctc aacatgttcc
     1081 tcaacttctc gatcaccccg ctgcgcgtct cggctctgct cggcatggcg acctcgctcg
     1141 tcagcctgtt cctgctggtg gcaatcgtcg tcgacaagct ctatgccaaa gccgaagtga
     1201 cggccggcat cccgacggtg ctggtcgtgg tcgtgttctt cgccgggatc cagctggtga
     1261 tcctcgggac gatcggcgag tacctcgggc gtctgttcct cgaccattcc aacgcgccgc
     1321 agttcgtcgt gcgctacgtc aagcgcggag gggcgcgcga tggatgagtt ctaccgtggg
     1381 aagcgcgtgc tgatcacggg cggggtcggg ttcatcggca gcaacctcgc gatacggctg
     1441 gtcggcgccg gcgcacgggt ccagctggtc gactcgatgc tgccggcgta cggcgccgat
     1501 ctggccagtc tcgagccggt acgcgatcgg ctccgcgtca acttctccga cgtgcgcgac
     1561 cagcacagcc tgagctatat cgtgcgcggc gaggacgtga tcttcagcct ggccggacag
     1621 gtcagccatt ccgacagcat gcacgacccg ctgaccgacc tcgaaatcaa ctgtcgcagc
     1681 cagctctcgc tgctcgagtg ctgccggcaa ggcaatccgg acgcgacgat cgtcttcgcc
     1741 agcacgcgcc agttgtacgg ccggccgcgc tacctgccgg tcgacgaggg gcatccgctc
     1801 aagccggtcg acgtcaacgg gatcaacaag ctcgccgccg aaatgtacta ctcgctgtac
     1861 cacgaggttc acggcatcgg tacggtcagt ttgcgcatga ccaacaccta cggcccgcgg
     1921 atggacatca cgaccgggcg caaggggttc gtcggcgtgt tcatcgaccg cgcgctgcgc
     1981 ggcgagacga tccgcgtgtt cggcagcggc gagcaacgtc gcgacttcaa ctacgtcgac
     2041 gacgcgttgg acgcgctgct gatggcgggc gagagaccgg agctgcgtgg cggcgtctac
     2101 aacctgggcc accccaagac ctactcgctg atcgaattcg tcgagacgct ggctcgcgtg
     2161 cacccgctcg agttcgagtg cgtgcccttc ccgcccgagg cggccgcaat cgacatcggt
     2221 gactaccagg gcgatttcac gcgcttcgag cgggcgaccg gctggcggcc gatcgtcgat
     2281 ctcgaggagg gcttgcgccg cacggtcgcc tacttccgcg gtcgcggcgg cccgcacgac
     2341 gcccgatgat cccgttcttc gactaccggc cgcagtacgc gcggctgcgc tccgggatcg
     2401 acgacgccat ccaacgcgtg ctcgactccg gccagctgat cctcggcagc gaggtcgacg
     2461 cgttcgagag cgagttcgct tcgttcgtcg gcgtgggcgg ggcggtcggt gtcaattccg
     2521 gtacggacgc gctgatcgtc gcgttgcggg cgctcggcat cggcgccggg gacgaggtga
     2581 tcaccgtagc caatgccggc acgccggtcg tggcggcgat ccgcgccacc ggcgccatgc
     2641 cgcgattcgt cgacgtcgat cccgtctcgc tgcagctcgc gcccgaacgg ctgggcgacg
     2701 cgctgtcgga gcgtacccgc tgcgtgatcc cggtccacct gtacgggcag cccgcgccga
     2761 tcgagacgat cgctgccttc gccgagcagc acgagctggc gctcgtcgaa gattgcgcgc
     2821 aggcgcacgg cgcacgggtc ggcggccggc acgtcggcac cttcgggcgg gtcggctgct
     2881 tctcgttcta cccgacgaag aacctcggcg ccttcggcga cggcggcatc tgcatcagcg
     2941 acgatccgga gctgctcgaa cgactgcggc tcgaacggat gtacggctac cgcggcgacc
     3001 gctcatcaca gatcgagggg atcaacaccc gcctcgacga gatccaggcg gcgattctgc
     3061 gggtcaagct gtcacgcctc gacggagcgc tcgacgagcg ccgccgtctg gcgcggcgct
     3121 atctcgacgg gctcgcgacg acctcgctga cgctgcccgc gccacccccg accgtcgagc
     3181 acgccttcca cctgttcgtc gtgcgccacg acgagcgcga tcgagtcggc gagcagctca
     3241 gccggtgccg catcggctgg gcggtgcact acccgcagcc ggtccatcgg atgccggcat
     3301 accgctttct cgcctacgac gagggccggc tgcccgtcac cgagcaggcc tcgcgatcgg
     3361 tcctgtcgtt gccgctctac cccgggctcg aggaccaggc cgtcgatcgc gtgatcgagg
     3421 cgctgacgag cctcgcgtg
//
LOCUS       DASPGN010000437         9509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_782555, whole genome shotgun sequence.
ACCESSION   DASPGN010000437 DASPGN010000000
VERSION     DASPGN010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782555"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1882
                     /locus_tag="VD788_12720"
     CDS             <1..1882
                     /locus_tag="VD788_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXV77173.1"
                     /translation="TLDTTRADRLGCYGYESAETPNFDRLADAAVLFEQAVSPVPTTL
                     PSHSTLFTGRYPQDHGVRYNLVYRLGPEAVTLAEYLHGAGFATAGFPSAYVLAPAYGL
                     DQGFDTYSADSIAPPGGDPPTDFNGLRAGRVVDDAIAWLDGRSESEKQFAWLHFYDPH
                     APYDPPFPYSSRYRGRPYDGEIAYMDAQFGRLLARLESSPAWAQTLLIVAGDHGEGLH
                     EHRERFHANLIYETTQRVPLFIRLPAGSARRIEEPVTLADITPTLLDLLGIEAAEKLR
                     GISLADVLRGDEPPRRDIYFESLAGAMNYGWQELRGIRYGPWKLIQSDDAQLFHLDDD
                     PGESSDLASLEGERVADMSAALSSLAEPLDGSSAAELAHDPVTSPETERFLASLGYVA
                     GGSGGSAEGAANPRELIDLEPELLAGQLSVGAGDWEAVEGLCRYVLERDPKNKWALGN
                     LVGALIRSDRFEEAQDTAVEIIRHYPDREQGYLLLARCYRAEERVDEAFRVLSEGLER
                     VGESAGLRYFYIVAGFEVDAEGLCEETVPRAVESHPDSARLTVMLARCEAGAGKTDAA
                     LDTLARAVELGFPALEQLKEGEEFEEVMRHPRFAELLAEVDRRRAEREKTAPEQAEPE
                     GS"
     gene            2072..2302
                     /locus_tag="VD788_12725"
     CDS             2072..2302
                     /locus_tag="VD788_12725"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HXV77174.1"
                     /translation="MLPTRHEVKVLNEVGARIFDLLDGSRSQAQIARMIADEFDVSVD
                     QANRDVAAFLDELRQAGMVSDEPEASGPGEVH"
     gene            2302..3378
                     /locus_tag="VD788_12730"
     CDS             2302..3378
                     /locus_tag="VD788_12730"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV77175.1"
                     /translation="MADTPYNVIRRKAFAKALPLNCQIEITYRCNHLCTFCYNSPTGE
                     KEMTTDQLFEALRKIRETGVLYVTLTGGEALCHKDFFKIAAEVRRLGMALRVYSNGYL
                     MADPKVVERLAALTPSEVEISIHGAKPETHDALTRITGSFAKTWQAVDNLVAAGLKVN
                     LKCPITRLNQDELFDIRDMGEARGLQVQFDAVITPKDDGNQDPLALRADDEIIAKYWS
                     DWYSELHRGALPPRANHCASEGGANCGTARSGFTIDPYGNILPCVAFRRSAGNILEVG
                     SLAEVWQKSPVLNEVRDLAVQVRQELDQHENGPYFTFCLGVAEVQTGNPKAMYPQAEL
                     NARAVRRHYELIQITDPDASAKSA"
     gene            3762..7982
                     /locus_tag="VD788_12735"
     CDS             3762..7982
                     /locus_tag="VD788_12735"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HXV77176.1"
                     /translation="MFSDRSFTNEHFSLTLSIPQPRLDIAADGSVVVEVDGLDAPRRH
                     AGAPDVPTRTVLVAIPPDARPTLVVGDGDDRVWRGIVPAPVPRRVVEIDEARRREVER
                     RGPLERQPLLDDAVREIRVRDRSIYDGAEPFPRSVAWFGNTGWLRDQRYVELHLAPVR
                     YDPTIGGLRSRDTLTIDVRFNSPSGAALDPAPADPRFEPVYRASFVNYEQSRQFRRRS
                     PDSRDVAVAAAASLGETGPRQRIRVDQDAVVRLDYPALQPTGLLAHDVGSWVLTHRGV
                     AVPMQVQDDGDGVIEPGEWIQFYGERLDDEPKTVLNTDFPILDLFEARDFTDVNTYFL
                     FVGTAGESSMTTRDAAPTLTRTPPTQFTERVHEEIDDSYRPLGAADPWYWLPTLTLSA
                     RSRVDTIELPGLVSGSEPVTARARMRGIDEDLDLGPDHPVRLSLLDAADTVLATTDDA
                     FDDRTIFDQELDWTWPGGGPQASDPVKVELEVLDGPAVCSGSPCNSVILDWIEIDYQR
                     AFSAVGDRLSFDWPDGDAEFVVGGLNDPSPEIFEITSAGGESPTQPVRLLGGQVSGSG
                     PFAVRFRVDQDPLVPDGTPRRFVVFGDGAVVAPADFTPDVVSDLRDQALQADLIVIAH
                     PDVLDAAPSSPLALLLAHRATAAGGGLSSKVVLLGDVEDEFNHGLPGPTAVHEFLRWV
                     MSTEPGEGWSDPRPSYVMLLGDASFDYKAGTAAGNFVPTQILFKDDPVLGLYASDNVM
                     SAVVGDDQLTDFMIGRIPAQSVDEANRMLLKVLDYEQNPPLGGWRNHTLFISDRGKAP
                     AIQSEGAQFELINSQSRAYMDEPPYTAHELRYLTDYFNTNDPNPWDTINGDLKDTING
                     VGGGPGGTSLVQFFGHGNFVLWSTDFFFDERINPPSDTFQDSMDLVNSTRLPWLMAHN
                     CLTGGFHASNSIGENWFKLDGGGSVAVFAPTGLSFNFVGGPTAFSIFDDVFGPAKARR
                     LGPIVAKAVAGLCANGSIEACQNYALQGDPATRLVLRDVGPAGALGAAASNQRVDLAW
                     DASPTPGVTYDLYRTDDLIRGTYARIGTALTQTTHADTAVINATTYYYYVVAVDGEGF
                     ESAWSNFNSDCATAGVDCVQATPLNPDPPLPPSDVTLVDPGTGETLRVSWAPNPENDL
                     AYYEVHYGIESGVYTETTRAGLVTELTLTGLVEGQMYHFVVTATNTSDLTSDPSDEAS
                     DFPVFSPGLDVPAYVDDLRVGRQGDDLVLEWTEVTTDVYGKPVDVVKFEILRGPSPDY
                     ATASLTKIGECVTPCSSFTDIGAAITPSDYHYRVRAADGEGNLSGLGGNPPDSTTLLI
                     GKDTEVPGKLVLTWSPVATTVDGSPVEVVHYLLYAADQPFSRADVRDGLLPAPTIVTT
                     TSFELTPPMQNRYYSVLAVDGRGNVSPF"
     gene            8046..8648
                     /locus_tag="VD788_12740"
     CDS             8046..8648
                     /locus_tag="VD788_12740"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV77177.1"
                     /translation="MLDSIPDRPGTRLADDADVDRGLVVRALDGDPRAFEELLERHQA
                     RVLRVVRLLGVRPEDREDVAQEVFVRVFRYLNGYRTRHAFAGWIYRVTVNAVHDHRGR
                     DYRRGREEVQWTPNLEDSPLAAQEPRGGDQDLILRRRLEQALGVLSERERAVFVLVEL
                     EGLTTADVARSLGITTITVRRHLGRARRSLQKTLRDPDGE"
     gene            8722..9219
                     /locus_tag="VD788_12745"
     CDS             8722..9219
                     /locus_tag="VD788_12745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77178.1"
                     /translation="MIWKPQKNREKSDQLDRALAAWHRSGAESPRLGDRARRAALAEA
                     ARARSTEALPAPFSSLFVPFGRLAVAAATPVVILSLMVGSVLLSDGALHRDTGIATSP
                     TRIDAVKTGDEVVFLIHNGSRRHTISKSEIAHDFAAEGSVTVTTGQFRDRLDSGAEIV
                     YYRID"
BASE COUNT         1581 a         3190 c         3204 g         1534 t
ORIGIN      
        1 cacgctcgac acgacgcgcg ccgaccgact cggctgctac ggatacgagt cggcggagac
       61 gccgaacttc gaccggctcg ccgacgcagc ggtgctgttc gagcaggcgg tctcgcccgt
      121 cccgacgacc ctcccgtcgc acagcacgct gttcaccggc cgctatccgc aggatcacgg
      181 tgtgcgctac aacctggtat accgactcgg accggaggcc gtcacactcg ccgagtacct
      241 gcacggcgcc gggttcgcga ccgctggctt cccgtcggcc tacgtgctcg ctccggccta
      301 cggtctcgac cagggcttcg acacctactc cgccgactcg atcgcgccgc cgggtggcga
      361 cccgccgacc gatttcaacg ggttgcgcgc cgggcgcgtc gtcgacgacg cgatcgcgtg
      421 gctcgacggt cgaagcgaga gcgagaagca gttcgcctgg ttgcatttct acgacccgca
      481 tgcgccgtac gatcccccgt ttccctattc ctcgcggtac cgcggtcgac cgtacgacgg
      541 cgagatcgcc tacatggacg cgcaattcgg ccgcctgctc gcgcggctcg aatcgtcgcc
      601 cgcttgggcg cagaccctgc tgatcgtcgc cggcgaccac ggcgaggggc tgcacgagca
      661 ccgcgagcgg ttccacgcca acttgatcta cgagacgacg cagcgtgttc cgttgttcat
      721 ccgcctgccg gcgggctcgg cccggcggat cgaagagccg gtcacgctcg ccgacatcac
      781 accgaccctg ctcgacctgc tggggatcga ggcggcggag aagctgcgcg ggatcagcct
      841 ggccgacgtg ttgcgcgggg acgagccgcc gcggcgcgac atctacttcg agagcctggc
      901 cggcgcgatg aactacggct ggcaggagct gcgcggcatc cgctacggcc cgtggaagct
      961 gattcaatcc gacgacgcgc agctgttcca tctcgatgac gaccccggcg agtcgagcga
     1021 cctcgcctcg ctcgagggag aacgggtggc ggacatgagc gcggcgctgt cctcgctcgc
     1081 cgagcccctc gacggcagtt cggcggccga gctggcccac gacccggtga cgagccccga
     1141 gacggagcgc ttcctcgcca gcctcggcta cgtcgccggc ggctcgggcg gcagtgcgga
     1201 gggcgcggcc aacccgcgcg agctgatcga cctcgagccc gagttgctgg ccggtcagct
     1261 ttccgtcggc gccggcgact gggaggcggt cgagggactc tgccggtacg tgctcgagcg
     1321 ggaccccaag aacaagtggg cgctcggcaa cctcgtcggc gcgctgattc ggtcggatcg
     1381 tttcgaggaa gcgcaggata cggccgtcga gatcatccgg cattaccccg atcgcgagca
     1441 gggctacctg ctgctcgcac ggtgctaccg ggccgaggag cgggtcgacg aagcgttccg
     1501 cgtgctgtcc gagggtctcg agcgcgtcgg ggagtcggcg ggtctgcgtt acttctacat
     1561 cgtcgccggc ttcgaggtcg atgccgaggg gctgtgcgag gagaccgtgc cgcgagcggt
     1621 cgaatcgcac ccggactcgg cgcgtctgac ggtgatgctc gcgcggtgcg aggcgggggc
     1681 ggggaaaacg gacgcggcat tggacacgct ggcccgagcg gtcgagctcg gcttccccgc
     1741 gctcgaacaa ttgaaagaag gcgaggaatt cgaggaagtc atgcgccacc cgcgcttcgc
     1801 ggagttgttg gccgaggtcg accggcggcg ggccgagcgc gaaaagaccg caccggagca
     1861 ggccgagccg gaaggttcgt gagttgtcat gtccgagcga tcaccgtaca ctttagccga
     1921 ccgcggaagt atctgaccga aaaggtacgg attaaaaaaa gtccgtcccc cgcgcggcgg
     1981 cgggacagga gtcgacatga tgggcggcca gaacccccgg cggcaccctc acacggcgtt
     2041 tcgctcgcag ggcgaagacg gagggctcgt cgtgctaccc acgcggcacg aggtgaaggt
     2101 gctcaacgaa gtcggggcac ggatcttcga cctgctggac ggcagccgct cccaagcaca
     2161 gatcgcccgg atgatcgccg acgagttcga cgtcagcgtc gaccaagcga accgcgacgt
     2221 cgcggccttc ctcgatgagc tgcgccaggc cggaatggtc tccgacgagc cggaagcgag
     2281 cgggcccggc gaggtgcatt gatggccgac acgccgtaca acgtgatccg gcgcaaggca
     2341 ttcgcgaagg cgttgccgct caactgccag atcgagatca cctaccggtg caatcatctg
     2401 tgcacgttct gctacaactc gccgaccggc gagaaggaaa tgacgaccga tcagctgttc
     2461 gaggcgttgc gcaagatccg cgaaaccggc gtgctctacg tcacgctgac cggcggcgag
     2521 gcgttgtgcc acaaggactt cttcaagatc gcggccgaag tgcggcggct gggcatggcc
     2581 ctgcgagtct actcgaacgg ctatttgatg gccgacccca aggtggtcga gaggctcgcg
     2641 gcgttgacgc cgtccgaggt cgagatctcg atccacggtg cgaagcccga gacccacgac
     2701 gcgctcacgc gcatcaccgg ttcgttcgcc aagacctggc aggccgtgga caacctggtc
     2761 gccgccgggc tgaaggtgaa cctcaagtgc ccgatcacgc ggttgaacca ggacgagctg
     2821 ttcgacatac gcgacatggg cgaggcccgc gggctccagg tccagttcga cgcggtcatc
     2881 acgcccaagg acgacggcaa ccaggacccg ctggcgctcc gcgccgacga cgagatcatc
     2941 gccaagtact ggagcgactg gtactccgag cttcatcgcg gggcgttacc gccgcgggcc
     3001 aaccactgcg cgtcggaggg aggtgccaac tgcgggaccg cgcgctcggg cttcaccatc
     3061 gatccgtacg gcaacatcct gccgtgcgtc gcgttccgcc gctcggcggg aaatatcctc
     3121 gaggtcggca gcctggcgga agtatggcaa aagtcaccgg tgctcaacga ggtgcgtgac
     3181 ctggcggtcc aggttcgcca ggagctcgat caacacgaga acggtccgta tttcaccttc
     3241 tgcctcgggg tcgccgaggt acagaccggc aatccgaagg cgatgtaccc tcaagccgag
     3301 ctaaacgcgc gggccgtccg gcgccattac gaactgatcc agatcaccga tcccgatgcc
     3361 agtgcaaaat ctgcttgaaa aggctaaaac gccagcgtat atctaccggc ctggacgaac
     3421 tgtcctctgt tcggcccggc ttgagaattg gctctgagat ttgggaataa cccaatacag
     3481 ggggaggcga atgaagaaga actacgagcg tccggccatc atccacacgg aaaagctgga
     3541 ggcgcgcgcg gtccagtgca tgaaggccga tgacggctgc ggcgccgaca tgggcccgat
     3601 ccagagctga cgtctaccgt ccttagacag aagggaccac agacagggtc ccccgacggc
     3661 ttatggcagc gagcaagtac attgtcgggg gggttttact agttctggct cttactgccc
     3721 ggggagcgga acctgaacaa ggtcacgctc cccgggctgt cgtgttcagc gaccgttcat
     3781 tcacgaacga gcacttctcg ctgacgctct ccatcccgca accgaggctc gacatcgccg
     3841 ccgacggctc ggtcgtcgtc gaggtcgacg ggctcgatgc gccacggcgc cacgcgggtg
     3901 cgcccgacgt tccgacccgg acggtgctgg tcgcgatccc gcccgacgcg cgacccacgc
     3961 tcgtcgtcgg tgacggcgac gaccgcgtgt ggcgtgggat cgtgccggcg cccgtgcccc
     4021 gtcgcgtcgt cgagatcgac gaggctcggc gtcgcgaggt cgaacgacgc gggccgctcg
     4081 agcggcagcc gttgctcgac gacgcggtcc gcgagatccg ggtgcgagat cgatcgatct
     4141 acgacggagc ggagccgttc ccgcggtcgg tcgcgtggtt cgggaacacc gggtggctgc
     4201 gggaccagcg gtacgtcgag ctgcacctcg cgcccgtgcg ctacgacccc acgatcggcg
     4261 gcctgcgctc tcgcgacacg ctgaccatcg acgtgcgctt caacagtccg tccggcgcgg
     4321 cgctcgatcc ggccccggcc gatcctcggt tcgagccggt ctaccgcgcg tctttcgtga
     4381 actacgagca gtcccgccag tttcggcgcc gatcgccgga ctcgcgcgac gtcgccgtag
     4441 ccgccgcggc ctcgttgggc gagaccggtc cgcgccagcg gatccgggtc gatcaggacg
     4501 cggtcgtgcg actcgactac cccgcgctcc aaccgaccgg tcttctggcg cacgacgtcg
     4561 ggtcgtgggt cttgacccat cgcggggtcg cggtgccgat gcaggtccag gacgacggcg
     4621 acggcgtgat cgagcccggc gagtggatcc agttctacgg cgagcgcctc gacgacgagc
     4681 cgaagaccgt gctgaacacc gacttcccga tcctcgatct gttcgaggcg cgagatttca
     4741 ccgacgtcaa cacctatttt ctgttcgtcg gcaccgccgg agagtcgtcg atgacgacac
     4801 gcgacgccgc gccgaccttg acgcggactc ccccgacaca attcaccgag cgcgtgcacg
     4861 aagagatcga cgacagctac cgtccgctgg gtgccgccga cccgtggtac tggctcccga
     4921 cgctgacgct gtcggcccgc tcgcgcgtcg acacgatcga gctgccgggt ctggtctcgg
     4981 gcagcgagcc ggtgacggcg cgcgcgcgca tgcgcggtat cgacgaagac ctcgacctgg
     5041 gccccgacca tcccgtccga ctcagtctgc tcgacgccgc cgacaccgtg ctggcgacca
     5101 cggacgacgc cttcgacgac cgcacgatct tcgaccaaga gctcgactgg acctggcccg
     5161 gaggcggacc gcaggccagc gatccggtca aggtcgagct cgaggtcctc gacggcccgg
     5221 cggtctgctc cggcagcccg tgcaactcgg tgattctcga ctggatcgag atcgactacc
     5281 agcgtgcgtt cagcgcggtc ggcgacaggc tgagcttcga ctggccggac ggcgacgccg
     5341 agttcgtcgt cggcggcctg aacgacccgt cgcccgagat tttcgagatc acctcggccg
     5401 gcggcgagtc gccgacgcag cccgtccggc tgctcggcgg ccaggtcagc ggctcggggc
     5461 cgttcgcggt ccgcttccgc gtcgatcaag atccgctggt gccggacgga acgcctcgcc
     5521 gattcgtcgt cttcggcgac ggggcggtgg tcgcgccggc ggacttcact cccgacgtcg
     5581 tctccgacct gcgggaccag gcgctgcagg ccgacctgat cgtcatcgcc catcccgacg
     5641 tgctcgacgc ggcgccgtcg agcccgctcg cgttgctgtt ggcgcaccgg gcgacggcgg
     5701 cgggcggcgg tctgagctcg aaggtcgtcc tgctcggcga cgtcgaggac gagttcaacc
     5761 acggcttgcc cggtccgacc gccgtgcacg agttcctccg ctgggtgatg tcgaccgagc
     5821 ccggcgaggg ctggtccgat ccgcggccgt cctacgtcat gctgctcggt gacgcgtcgt
     5881 tcgactacaa ggccggcacc gccgccggca acttcgtccc gacgcagatc ctgttcaagg
     5941 acgacccggt gctggggctg tacgccagcg acaacgtgat gtcggcggtc gtcggcgacg
     6001 accagctgac cgacttcatg atcgggcgga tcccggccca atcggtcgac gaagcgaacc
     6061 gcatgctgct caaggtgctg gactacgagc agaacccgcc gctcgggggt tggcgcaatc
     6121 acactttgtt catctcggac cgcggcaagg cgccggcgat ccagagcgaa ggcgcgcagt
     6181 tcgagctgat caacagccag agccgggcgt acatggacga accgccgtac acggcgcacg
     6241 agctgcgtta cctgaccgac tacttcaaca ccaacgaccc gaacccgtgg gacacgatca
     6301 acggcgacct gaaggacacg atcaacggcg tcggcggcgg gccgggcggg acgtcgctgg
     6361 tccagttctt cggtcacggc aacttcgtgt tgtggtcgac cgactttttc ttcgacgaac
     6421 ggatcaatcc gccgtcggac acattccagg attcgatgga cctggtgaac tcgaccaggc
     6481 tgccctggct gatggcgcac aactgcctga cgggcggttt ccacgccagc aactcgatcg
     6541 gtgagaactg gttcaagctg gacggcggcg gctcggtcgc ggtcttcgcg cccaccggtc
     6601 tgtcgttcaa cttcgtcggc ggcccgaccg cgttctcgat cttcgacgac gtcttcgggc
     6661 cggccaaggc gcgtcgcttg gggccgatcg tcgccaaggc agtcgccggt ctgtgcgcca
     6721 acggatcgat cgaggcctgt cagaactacg ccctgcaggg cgacccggcg acccgcttgg
     6781 tcttgcgcga cgtcggtccc gccggcgccc tcggcgccgc ggccagcaac cagcgggtcg
     6841 atctggcctg ggatgcgagc ccgacgccgg gagtgaccta cgatctctat cgcaccgacg
     6901 acctgattcg cggcacctac gcgcgcatcg gcaccgcgtt gacgcagacg acccacgccg
     6961 acacggcggt gatcaacgcg acgacctact actactacgt ggtggcggtc gacggcgagg
     7021 gcttcgagag cgcgtggtcc aacttcaaca gcgattgcgc gacggccggc gtggactgcg
     7081 tccaggccac gccgttgaac cccgacccgc cgttgccgcc gagcgacgtg acgctcgtcg
     7141 atccgggcac cggtgagacg ctgcgagtga gctgggcgcc gaacccagag aacgacctgg
     7201 cctactacga ggtgcattac gggatcgagt ccggcgtcta caccgagacg acacgcgccg
     7261 ggctcgtgac cgaactcacc ctgaccggtc tggtcgaagg gcagatgtac cacttcgtcg
     7321 tgacggcgac gaacacgtcg gatctcacct ccgacccctc ggacgaggcg tcggacttcc
     7381 cggtcttctc tccggggctc gacgtgccgg cctacgtcga cgatctccgc gtcggccggc
     7441 agggcgacga cctggtgctc gaatggaccg aggtcacgac cgacgtgtac ggcaagccgg
     7501 tcgacgtcgt caagttcgag atcctgcgcg gtccgtcacc cgattacgcg accgcgagcc
     7561 tgaccaagat cggcgagtgc gtgacgccgt gctcgtcctt caccgacatc ggcgccgcga
     7621 tcacgccgag cgactaccac tatcgggtgc gcgcggccga cggggagggc aacctcagcg
     7681 ggctcggcgg caatccaccc gactcgacca cgctcttgat cggcaaggac acggaagtcc
     7741 ccggtaagct ggtcttgacc tggtcgccgg tcgcgacgac ggtcgacggg agcccggtcg
     7801 aggtcgtgca ctatctgctg tacgcggcgg accaaccgtt ctcccgcgcc gacgtccgcg
     7861 acgggctgct gcccgcgccg acgatcgtga cgacgacctc gttcgaactg acgccaccga
     7921 tgcagaatcg ctattattcg gtcctcgccg tcgatggtcg gggaaacgtc agtccgttct
     7981 gaggggggcg atcgccgcgc atggctgtta cggattcgtc ggccgcggat cgacgaggtc
     8041 gcgtcgtgct cgattcgatc ccggatcggc ccgggacccg gctcgccgac gatgccgatg
     8101 tcgaccgcgg cttggtcgtg cgtgcgctcg acggagaccc gcgcgccttc gaggagctgc
     8161 tcgaacggca ccaggcccgg gtgttgcggg tcgtccgtct gctcggggtg cgacccgagg
     8221 accgcgagga cgtggcccag gaggtcttcg tccgggtctt ccgctacctg aacggctacc
     8281 ggacgcggca cgccttcgcc ggctggatct accgcgtcac agtcaatgcg gtccatgacc
     8341 atcgcgggcg ggactaccgt cgagggcgcg aggaggtcca gtggacgccg aatctcgagg
     8401 attcacccct ggccgcgcag gagcctcgcg ggggtgatca ggacctgatc ctcaggcggc
     8461 gactggaaca ggccctcggg gtgctcagcg agcgcgagcg ggcggtcttc gtgctggtcg
     8521 agctcgaggg actcacgacg gccgacgtcg cgcgctcgct cgggatcacg acgatcaccg
     8581 ttcgccggca cctcgggcgg gcccgcaggt cgctgcagaa gacgctccgc gacccggacg
     8641 gagaataaag gtccttgccg ttgaacgatt cggccccggc cggaggtatt caaccgtagc
     8701 cgccaggagg acgacgatcc catgatttgg aaaccgcaga aaaaccgcga aaaaagcgac
     8761 cagctcgacc gcgcgctcgc cgcgtggcac cgaagcggag cggagagtcc gcgcctgggc
     8821 gatcgagcgc ggcgggccgc cctcgccgag gccgcgaggg cgcgctcgac cgaagctctt
     8881 cccgctccgt tttccagcct attcgttcct ttcgggcgtc tcgccgtcgc ggccgcgacg
     8941 ccggtcgtga ttctctcgtt gatggtcggc tcggtgctac tgtccgatgg cgctctccac
     9001 cgtgacaccg gtatcgcgac ctctcccaca cggatcgatg ccgtgaagac cggcgacgag
     9061 gtcgtcttct tgatccacaa cgggagtcgg cgtcacacga tctcgaaatc cgagatcgcg
     9121 cacgatttcg cggcggaggg ttcggtgacg gtgaccaccg gccagttccg cgatcgtctg
     9181 gattcggggg ccgagatcgt ttactaccgc atcgattgat ttcgttcagg tgtatttttt
     9241 gaattatcgt aatttagctg ctcgacttgg tggttccgag atggcgattt ctttgcttga
     9301 cgtttcgagc cataccggta catttacggg cgcgcagggc gcccgaccgg tgagttcggg
     9361 ggggcagagg acacgtcgta tcgagttcgc cgggaacggt cagcgctgac tgcggaggag
     9421 attcgatgaa gaagagaatg gttgtcgggc tcgtgggtgg cctcgccgtc ctgctcgtcg
     9481 caggtgggct catggcctcg aacatgggc
//
LOCUS       DASPGN010000438         4509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1719568, whole genome shotgun sequence.
ACCESSION   DASPGN010000438 DASPGN010000000
VERSION     DASPGN010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719568"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VD788_12750"
     CDS             <1..493
                     /locus_tag="VD788_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77179.1"
                     /translation="LPFAPVAAVGGILLVTGAATLVLVRGRRLLPAVVAPAVGFALAY
                     LVTAFLVYPAADARKSARPLALRMAEVSAASRAAGHPVLAFSTGNLPEPLAFYSDGVY
                     TWETSDLELLERHLLQQAEVYAVVSARDVERLSAAARERMTIVERYELGKPIWLVSNV
                     RAD"
     gene            complement(501..2882)
                     /locus_tag="VD788_12755"
     CDS             complement(501..2882)
                     /locus_tag="VD788_12755"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV77180.1"
                     /translation="MNRLAGCLALCSSIVFGTVSAADWPHLRGPALDGSTVSGGALSG
                     ERVELELAWTAPIGSGYSGIVAADDRVVTMYSADGTDWVAAHEAKSGKVIWRHSLGDA
                     YPGRDGADDGPLSSPLIDEGRVHALGPRGTLYTLRLDDGKELWQVKLTTEFGSIEPDF
                     GFTTTPLVEGKVLILQTGGPRGKAISGLDKKTGRPVWSLGDDKVDYQSPATLTLAGQR
                     QVVAVSGKRLQGIEPSNGKILWSHDLGEEAYVESASPTPAGTDRFLIFSRGEAVVFEL
                     GKSSDGFGLKEVFRTKDLGGTYALPVFHDGHLYGFRNQFLSCVNADSGELVWKSRPPG
                     GRGLILVDGHLVVFGAEGNVVVAEATPAGYREQAKLKVLEGSGYTWPSFAADRIYVRN
                     LDTLVAVTVTAGSAGDAPESVGATPAADHDFGRWVASVESSADAAAMIDEFMKRHEQL
                     PVVEGNWVHFIYRGEVTDIAVFGTMIPTANPDAMTRIAGTDLYHASYELQPAGRWEYT
                     FVVDYDQRVPDPHNPRTAPSLRGDERVSVVTTAGYVEPSYLGAADANARGTLETVQFD
                     GEKYGDARELTVYLPRGYADGDRAYPLLIVHEGPDWLEKGLMVNSLDNLIGTTITPLV
                     AVFVPPADEWWLEAGGSQTDAYARMLAEELVPWLSQKFRLIDEAQARAVMGSRNYGLA
                     AAYVAWRYPRVFGNVAVQSVALGLGAEAELFGMIERADGDPKRVYLDWNRYEYRNPDS
                     GVDIAADNRRLAEVLERSGTPFSGGEVLDSYGWGGWRARTDRLLATFFPTSRQ"
     gene            complement(2948..>4509)
                     /locus_tag="VD788_12760"
     CDS             complement(2948..>4509)
                     /locus_tag="VD788_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV77181.1"
                     /translation="LLALSLNRRFELALISIVVTGVTAAVPMQQVKFTEQQEEERSGF
                     FVGVEMPESTTLDEAEQWFLEAEKIVEGKQDELGLEGWFLFHRKTFGEIQGWFTQPRT
                     VDVSAKQATKIVIDALPKRPGFTFTTGEDREEADEGDLATYVVTLNGEDWDQLGQVTD
                     ALEQVLVKVPGVLGSKGAADQANNELALVVDRERAQQLAVNPQVVAGLVSYALRGTPL
                     PKFHKDGKEIPVRVRFREEDRESLSELDSFLVPTDNGGFLPVSALTEAKVLPTKQSIV
                     RRGKRIARSIALELEEGKEDETRTSLNAVIAGVDLPEGVEFGSDRRTEQLNEDLAGLR
                     FAALLSIVFIYLLMGFLFESFVLPMSILFTIPLSAIGVWWIHFVVGYDIDFLGFVAIV
                     LLIGVVVNNGIVLIDYVNRLRDRGIDRHQALLTATDRRFRPIMMTAITTIGGMIPLAF
                     AGANSIGLSYTSFSLTLIGGMTTATLLTLLVVPVLYTVFDDIRQVFVVALARAMARTR
                     GEDDRQTEVVA"
BASE COUNT          729 a         1568 c         1455 g          757 t
ORIGIN      
        1 actgcccttc gccccggttg cggcagtcgg cgggatcttg ctcgtcaccg gggcggcaac
       61 cctggttctc gtccgcgggc gccgcctgct gccggcggtc gtcgctccgg ccgtgggctt
      121 cgcgctggct tacctcgtga cggcgttcct ggtctacccc gccgccgacg cgcgcaaatc
      181 ggcgcgcccg ctcgcgctgc gcatggccga ggtcagcgcg gcgtcacgtg ccgccggcca
      241 tccggtgctc gcgttcagca cgggaaacct gcccgagccg ctggcgttct acagcgacgg
      301 ggtctacacg tgggaaacca gcgacctcga gctgctcgag cgccatctgc tgcagcaggc
      361 ggaagtctac gccgtcgtca gcgcgcgcga cgtcgagcgg ctgtcggccg cggcgcggga
      421 acggatgacg atcgtcgagc gctacgagct gggcaagccg atctggctgg tctcgaacgt
      481 acgtgccgat tagacgaggc tcactgccgt gacgtgggaa agaacgtcgc cagcagacga
      541 tcggtcctcg cgcgccaacc gccccagccg tagctgtcga gtacctcccc gccgctgaac
      601 ggcgtcccgc tgcgttccaa cacttcggcc aaccgccggt tgtccgccgc gatgtcgacg
      661 ccgctgtcgg ggttgcggta ctcgtagcgg ttccagtcga gatagacccg tttggggtca
      721 ccgtcggcgc gttcgatcat tccgaacagc tcggcctcgg cgccgagacc gagcgcgacg
      781 ctctgcaccg cgacgttgcc gaagacgcgg ggatagcgcc acgcgacgta ggccgcggcc
      841 aacccgtagt tcctcgatcc catcacggcg cgtgcctgcg cctcgtcgat cagccggaac
      901 ttctgcgaaa gccacggcac gagctcctcg gccagcatcc gggcgtaggc gtcggtctgc
      961 gacccgccgg cctcgagcca ccactcgtcg gccggcggca cgaagaccgc gacgagcggc
     1021 gtgatcgtgg tcccgatcag gttgtcgagg ctgttgacca tcagcccctt ttccaaccag
     1081 tccggtccct cgtggacgat caacaacggg taggctcggt cgccgtccgc gtagccgcgc
     1141 ggcaggtaga cggtgagctc gcgggcgtcg ccgtacttct cgccgtcgaa ctgcacggtc
     1201 tcgagcgtcc cgcgggcgtt ggcgtcggcc gcaccgaggt aggacggctc gacgtagccc
     1261 gccgtcgtga cgaccgacac gcgctcgtcg ccgcgaagcg acggcgccgt tcgtgggttg
     1321 tgcggatcgg gtacgcgctg gtcgtagtcg acgacgaagg tgtactccca ccggccggcc
     1381 ggctggagct cgtacgacgc gtggtacaaa tcggtgcccg cgatccgcgt catcgcgtcc
     1441 gggttcgccg tcgggatcat cgtaccgaaa acggcgatgt cggtaacctc tccgcgatag
     1501 atgaagtgaa cccagttccc ctcgacgacc ggcaactgct cgtgtcgctt catgaactcg
     1561 tcgatcatcg ccgccgcgtc ggccgacgat tcgacgctgg ccacccagcg gccgaagtcg
     1621 tgatcggcgg cgggggtcgc cccgacggac tccggcgcgt cgccggccga tcctgccgtg
     1681 acggtgacgg cgaccaacgt gtccaggttg cgcacgtaga tgcggtcggc ggcgaaactg
     1741 ggccaagtgt agccgctgcc ctcgagcacc ttcagcttgg cctgctcccg gtagcccgcg
     1801 ggggtcgcct cggcgacgac gacgttgccc tcggcgccga agacgacgag gtgtccgtcg
     1861 accaggatca gcccgcgccc gccgggcggt ctcgacttcc acaccagttc gccggagtcg
     1921 gcgttgacgc aactcaggaa ctggttgcgg aagccgtaga ggtgtccgtc gtgaaagacc
     1981 ggcagcgcat aggtcccgcc caggtctttc gtgcggaaca cctctttcag gccgaacccg
     2041 tcgctcgact tgcccagctc gaagacgacg gcctcgccgc gcgagaagat caggaaccgg
     2101 tcggtgccgg ccggggtcgg gctcgcgctc tcgacgtagg cttcttcgcc gaggtcgtgg
     2161 ctccacagga tcttcccgtt cgacggttcg attccctgca gccgcttgcc gctgaccgcg
     2221 acgacctgtc gctgcccggc caaggtcagt gtcgccggag attggtagtc gaccttgtcg
     2281 tcgccgagcg accagacggg gcggccggtc ttcttgtcca gtccgctgat cgccttgcct
     2341 cgcgggccgc ccgtctgcag gatcagcacc ttgccttcga cgagcggggt cgtggtgaag
     2401 ccgaaatccg gctcgatcga gccgaactcg gtagtcagct tgacctgcca cagctccttg
     2461 ccgtcgtcga gacgcagcgt gtagagcgtc ccgcgcgggc cgagcgcgtg cacccggccc
     2521 tcgtcgatca acggcgagct caacggaccg tcatccgcgc cgtcacggcc cggataggca
     2581 tcgcccagcg aatgacgcca gatcaccttt cccgatttcg cctcgtgagc cgcgacccag
     2641 tcggtaccgt cggcggagta catcgtcacc acgcggtcgt cggcggcgac gatgccggag
     2701 taaccggagc cgatcggcgc ggtccaggcc agctcgagct cgacacgctc gccggacagc
     2761 gccccgcccg aaacggtcga accgtcgagc gcggggccgc gaaggtgcgg ccagtcggcg
     2821 gccgacaccg ttccgaagac gatcgacgag cacagcgcca gacaacctgc gagacggttc
     2881 attcgcttct cctcccttcc gcacagacta caccagcgag cgcaatccgt ctcacggaga
     2941 cgcgatacta cgcgacgact tccgtttgcc gatcgtcttc gccgcgggtg cgcgccatcg
     3001 cccgcgcgag cgcgaccacg aacacctgcc ggatgtcgtc gaagacggtg tagagcaccg
     3061 gcacgacgag cagggtcagc agcgtcgcgg tcgtcatccc gccgatcagc gtcagactga
     3121 agctggtgta gctcaaaccg atgctgttcg caccggcgaa ggccagcggg atcatgccgc
     3181 cgatcgtggt gatcgcggtc atcatgatcg ggcggaagcg tcgatccgtc gcggtcagca
     3241 acgcttggtg gcggtcgatg ccgcggtctc gcagccgatt gacgtagtcg atcaacacga
     3301 tgccgttgtt gaccaccaca ccgatcagca ggacgatcgc gacgaagccc aggaaatcga
     3361 tgtcgtagcc gacgacgaag tggatccacc acacgccaat ggcggagagc gggatggtga
     3421 acaggatcga catcgggagg acgaagctct cgaacaggaa gcccatcagc agatagatga
     3481 acacgatcga caacagcgcg gcgaagcgca aaccggccag gtcttcgttc agctgttcgg
     3541 tgcgcctgtc gctgccgaac tccacgccct cgggaagatc gaccccggcg atgacggcgt
     3601 tgaggctggt cctcgtttcg tcttccttgc cttcctcgag ctcgagtgcg atcgaacggg
     3661 cgatgcgttt cccgcgacgg acgatgctct gcttggtcgg taacaccttg gcttcggtca
     3721 gggcggacac cggcaggaaa ccgccgttgt cggtcggcac gaggaagctg tccagttcgc
     3781 tcaaactctc acggtcctct tccctgaagc gcacgcggac cgggatctcc ttgccatcct
     3841 tgtggaactt gggcagcggc gtgccgcgca gcgcgtagct gaccaacccg gcgactacct
     3901 gcgggttgac cgccagttgc tgcgcccgct cgcggtcgac gaccagcgcc agctcgttgt
     3961 tggcctggtc cgccgcgccc ttgctgccga gcacgccggg gaccttcacc agcacctgct
     4021 ccaacgcgtc ggtcacctgg cccagctgat cccaatcctc gccgttgagc gtgacgacgt
     4081 aggtcgcgag atcgccctcg tcggcctcct cgcggtcctc accggtcgtg aacgtgaacc
     4141 cgggtcgctt cggcagcgcg tcgatcacga tcttggtcgc ctgcttggcg ctcacgtcga
     4201 ccgtgcgcgg ctgggtgaac caaccctgaa tctcgccgaa cgtcttgcgg tggaaaagaa
     4261 accagccctc gagccccaac tcgtcctgct tgccctcgac gatcttctcc gcctcgagga
     4321 accactgctc ggcctcgtcg agcgtcgtgc tctcgggcat ctcgacgccg acgaagaaac
     4381 cggaccgctc ttcttcttgc tgctcggtga acttcacctg ctgcatcggc accgccgcgg
     4441 tgactcccgt caccacgatc gagatcaacg ccaactcgaa gcgacgattc agcgaaagcg
     4501 cgagcagct
//
LOCUS       DASPGN010000439         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_665402, whole genome shotgun sequence.
ACCESSION   DASPGN010000439 DASPGN010000000
VERSION     DASPGN010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665402"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /gene="pgsA"
                     /locus_tag="VD788_12765"
     CDS             complement(<1..584)
                     /gene="pgsA"
                     /locus_tag="VD788_12765"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HXV77182.1"
                     /translation="MRLNLPTALTLFRIFLVPLLVVVLLAPPWATVWVRDTVRGMESV
                     EWLGDLAGWLSSWREVVAVVIFLVAAATDALDGYLARRRGEVTTLGTLLDPIADKLLT
                     ASAFIALVELRLAPAWMVVVIVGREFVVSGMRSVAASRGQVIGASRWGKYKTVSQVVA
                     ITLLILTSTLERWVPYGNLGRIALWVVILLAIGS"
     gene            complement(706..1461)
                     /locus_tag="VD788_12770"
     CDS             complement(706..1461)
                     /locus_tag="VD788_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77183.1"
                     /translation="MRTVILAGVTGVLLASAADVRAEVSVRTDRYGRYVTTQIHVTHT
                     ETRSDDGLWAYRGLAERGTQVLNPDGDRNGDLWPVIVESNLAPFHPWAVWSRFDGSGY
                     QIAWSVWTGSAWTEIKSLHQHTPGDDLDPDIAFDPAGNPSVVWSREEGGVSHVYWSRF
                     VKKGFTPPMAISETHEDGRYPQILSNDGRSMVVEYTGASGTVQRTVAFAVPGTITDDI
                     NPLDRHAGTTPVFTGKQNGAAEPQIDPRSRPQR"
     gene            complement(1467..>2154)
                     /locus_tag="VD788_12775"
     CDS             complement(1467..>2154)
                     /locus_tag="VD788_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77184.1"
                     /translation="LKNRHRLRPSTRRRTLLQLSYLYRELGDLYLASVLAGECLELAR
                     GEGDRLAEAGVLNTLANIEHDEGQPQAALVHYEAARRILLDIGGHHEMVATVGTNLGG
                     CLAAVGRFDDGLRLLDESYETARTAGFRRVASLSLTRMAEAWTARGDQDRALSLLAES
                     DALAGRTGEPYDDILFLNAYHRWKLARRSRNETREKIAFGRLRHLRSKLQRSFVEVEQ
                     FDRHVERIRR"
BASE COUNT          352 a          742 c          741 g          319 t
ORIGIN      
        1 atcgagccga tggcgagcaa gatcaccacc catagcgcaa tccgaccgag gttgccgtag
       61 ggcacccaac gctcgagcgt gctggtcagg atcaacagcg tgatcgccac gacctggctg
      121 accgtcttgt acttgcccca gcgcgaggcg ccgatcacct gcccgcggct cgcggcgacg
      181 ctgcgcatcc cgctgacgac gaactcgcgc ccgacgatca cgaccaccat ccacgccggg
      241 gccaacctga gctcgaccag cgcgatgaac gccgaggcgg tcagcagttt gtcggcgatc
      301 gggtcgagca gcgtgccgag cgtggtcacc tcgccccgcc ggcgcgcgag atagccgtcg
      361 agcgcgtcgg tcgccgcggc gacgaggaag atgacgaccg cgacgacttc gcgccagctc
      421 gacagccagc cggcgaggtc acccagccat tcgaccgact ccatgccgcg aaccgtgtcg
      481 cgcacccaga ccgtggccca cggcggtgcg agcaggacga ccacgaggag cggcacgagg
      541 aagatgcgaa aaagcgtcag cgcggtgggg aggttcaagc gcatccgatc ggtcgccgcc
      601 gccgtcgcaa gcttcgatcc cgcacgtact gcgggcgtgg acggcgccgc caaagtaaca
      661 tgcggcgcgt cgaggtggca atgcacggcc cgcggcgggg cggcgctaac gctgcggtcg
      721 actccgcggg tcgatctggg gctcggccgc gccgttctgc ttgccggtga agaccggcgt
      781 cgtgccggcg tgacgatcga gcgggttgat gtcgtcggtg atcgtcccgg gaacggcgaa
      841 cgcgaccgtc cgctggaccg tgccggacgc gccggtgtac tcgacgacca tcgaccgccc
      901 gtcgttggac aggatctgcg gataacggcc gtcctcgtgc gtttcggaaa tcgccatcgg
      961 tggagtgaag cccttcttga cgaaacggct ccagtagacg tggctgaccc cgccctcttc
     1021 gcggctccag acgaccgacg ggtttcccgc aggatcgaac gcgatgtcgg gatcgagatc
     1081 gtcccccgga gtatgctggt gcagcgactt gatctcggtc caggccgagc cggtccagac
     1141 cgaccaggcg atctggtacc cgctgccgtc gaagcggctc cagaccgccc acgggtgaaa
     1201 cggcgcgagg ttggactcga cgatcaccgg ccacagatcg ccgttgcggt cgccgtcggg
     1261 gttcagcacc tgggtcccgc gctcggcgag accgcgatag gcccacaacc cgtcgtcgct
     1321 ccgggtctcg gtatgggtca cgtggatctg ggtggtgacg tagcggccgt agcggtcggt
     1381 tcgtaccgac acctccgcgc gaacgtcggc ggccgacgcc agcaacacac cagtgactcc
     1441 ggccaagatc acggtacgca tcggcatcac ctccgaatcc gttccacgtg ccggtcgaac
     1501 tgctcgactt ccacgaagct gcgctgcaac ttcgagcgca ggtgacgcag acgcccgaag
     1561 gcgatcttct cgcgcgtctc gttgcgcgat cggcgggcca acttccagcg atggtaggcg
     1621 ttgaggaaca ggatgtcgtc gtacggctcg cccgtccgcc cggccagcgc gtcggactcg
     1681 gcgagcagcg acagtgcccg gtcctggtcg ccgcgtgcgg tccaggcctc ggccatccgg
     1741 gtcagggaca gcgacgcgac ccggcggaag ccggcggtcc tggcggtctc gtacgattca
     1801 tcgagcaggc gcagcccgtc gtcgaaccgg ccgacggccg caaggcagcc gccgaggttg
     1861 gtgccgacgg tggcgaccat ctcgtggtgg ccgccgatgt cgagcaggat ccgtcgcgcc
     1921 gcctcgtagt gcaccagcgc ggcctgcggt tgcccctcgt cgtgctcgat gttggccagg
     1981 gtgttgagca cgcccgcctc cgcgagccga tcgccctcgc ctcgcgccag ctcgaggcat
     2041 tcgccggcca gcacgctcgc caggtacagg tcgccgagct cgcgatagag ataactcagc
     2101 tgcagcagcg tccggcgccg ggtcgacgga cggagacggt ggcggttctt gagg
//
LOCUS       DASPGN010000440        20549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1602679, whole genome shotgun sequence.
ACCESSION   DASPGN010000440 DASPGN010000000
VERSION     DASPGN010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 20549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20549
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1602679"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VD788_12780"
     CDS             complement(<1..1394)
                     /locus_tag="VD788_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77185.1"
                     /translation="MAFSIIVCTPEHLSDPDLAIAAARAGEIGLLNLGPGQSATAWRV
                     ALARLARQAPRGSGWGVRWDGWVHARRSVAAFDRAASVGRLPILLLAGVDPAGLAERL
                     VWARERAERVLFEALDADAARAAAGAGYDGVVLKGNEAGGRVGSDSSFLLLQSLRGGA
                     GAPVWVQGAWGVDTVGAARVAGADGVVLAEELWLARESPFAEADRALFARLDGTESIA
                     LDDGEIHLRLFRQRDRETITAAQEELVRGGRPWDGLRDRLSDRSADGPSPLPLGQGIA
                     LARRLAERYETTGTMLDAFRTAAERLPREAARRETLAPASPLARAHGTRYPIVQGPMT
                     RVSDRAGFIEAVADNGALPFLALALMRGPEARRVLDETRERLGRRPWGVGILGFVPPE
                     LRREQLEVIRDVRPPFALIAGGRPSQAVALEGDGIATYLHVPSPGLLATFLDEGARRF
                     VFEGRECGGHVGPRS"
     gene            complement(1486..3147)
                     /locus_tag="VD788_12785"
     CDS             complement(1486..3147)
                     /locus_tag="VD788_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77186.1"
                     /translation="MTPNATFGTICGVAVVVSFQLVLLDPLHETMDLPVWDESNQMAW
                     GADFAEGSGTLGELAASPLYNLAYAVLAGAFGRVASVFAMKYLVTVLVALALWLMLDR
                     SSPHVAPPLWVAVLWVATVPNLDGSLLVYHFGLALFIAATAAGRDHPPLALVLLALCS
                     LARLEFLLPLVAYGGALAVRRWRRGGRAPAGRPRSLGLAACGVLLALAAYTAVHVDGW
                     DVGGKRAWHAFGQHYAVAEREAGRTSVNPWVDYHLIVRRDFGGSTSTAEAARANPAAF
                     ARHTATNLRRMPGAARSLATPLHFDRLRPAWVLVACWLLLPVVVALGSLRRLSSAVAA
                     RARMLGDRGLIAGLGALATLPCLVVRPKPIYLFPLVPLAGLTLVVLVSASWETVSSRS
                     LRRVLAAGTVVLTLGVAGLTLAAGRPYDRPSRVRPVLDKVARLERLWPDSRHTLVGVG
                     ASSYANYLGRRRCTAIEPFATVAGGAGAGVRVTLGSLVADHDPDAILINSLLVDSAQF
                     DRASLRMLNPAEWRPVAFGDELLLLRTRTADSPDERRADGVEPTG"
     gene            complement(3247..3429)
                     /locus_tag="VD788_12790"
     CDS             complement(3247..3429)
                     /locus_tag="VD788_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77187.1"
                     /translation="MIELRLTDEQAAVLRRILASEVSDLGMEIADTDSFDYRSGLKRT
                     KRLALELLERLDRNTA"
     gene            3576..4721
                     /locus_tag="VD788_12795"
     CDS             3576..4721
                     /locus_tag="VD788_12795"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HXV77188.1"
                     /translation="MRSSAIDNEDVRRLRGLRHLVGNAPILAVELTYRGTPRRIYAKA
                     EQLNLTGSIKDRMALHIVREGRRRGLLAAGARLVEATSGNTGISFAAVGRAMGHPVTI
                     FMPDWMSRERVELIRSLGAEIRLVSRAQGGFVGAIELAESYARRTPGAFLPRQFSNRD
                     NVAAHRETTGPEIWWQMRNHGLTPDVFVAGVGTGGTIMGVGQALRELSPTVKLHPLEP
                     ASSPTLRTGHCVGRHRIQGVSDEFIPPIVDLDFLDEVVDVEDGDAILMAQKLARELGL
                     GVGISSGANFLGALALVERYGGEANVVTVFPDDHKKYLSTDLLREEPAREGYLSPEVE
                     LLRFSAFKRVCRTCCEPSDCLVPQTSAGEDGVLPGCPLWRDRVESPV"
     gene            4879..6621
                     /locus_tag="VD788_12800"
     CDS             4879..6621
                     /locus_tag="VD788_12800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HXV77189.1"
                     /translation="MEALERAVAAVRDLIWKFGLPVGDETIPLVVIALLGTGVFLTLR
                     LGFVQVRRLGHGFAVATGKYDDPDEPGDVSHFQALATALSATVGIGNIAGVAIAIHWG
                     GPGALFWMWVTAALGMAVKYTEVTLAQQHRDVGPDLDANRWEGSVSGGPMYYIERGLG
                     PAWKPMAVFFAAMLGSTAFLTGNAIQANTVADTLETSIGMPVWVTGVITAGVVAAVIL
                     GGITRIGRVTGVLAPLMAAIYVSGALLILLLNVGAVGPAFLSIFTEAFNPSAGVAGTG
                     IGAILVTMMWGVNRGLFSNEAGQGSAPIAHAAAKTDEPVSEGVVALTEPFIDTIVICT
                     FTAMAIIVTGVYDDRVPTTIMLSGGDITYVAVDERGLRSEVAAPAEIRVEDGLQLVDP
                     TAPRLAWHDVPVERLAVDADRRVGFTGVIRPSARIARGDDGVEYTALYADAVESGAPL
                     TLLAFDRGLAPIGGFGPHVVTLSVLLFGISTAIAWSYYGDRCATYLLGPKAVLPYKMV
                     YVLMHFLGAGVSLQLAWDFGDVFLGIVILPNLIALVLLSGRVKEMTDSYFERRPWIEN
                     AEAHKRAVAARRTG"
     gene            6593..8989
                     /locus_tag="VD788_12805"
     CDS             6593..8989
                     /locus_tag="VD788_12805"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HXV77190.1"
                     /translation="MPSRRGGPAEPVRGLAAIAVGALLVAGCTGGERPTPGVVRLVDR
                     FAAASIDGSPPAAAPGTVGPGWTFEGAAAAGGPAAGWQTHRVDGFAVRDGLLHGRPRD
                     ETSLIHAAVDEPADGADLLHAVEIAIRSEAGGELAIGFSEDDELDLDEVLDNPFPWPL
                     RTPLVAAPTVERYSLRPTRATHLSSLRHLLIRPSGEFAIESVRVVSRREHLAAIESGV
                     GWHGMEDVFREAIVTRSPEVARFEIDLPERPWLDLAVATIEEGAATFRVEARSAGEPP
                     IVFERTVSRPHRWSPVRIDLAPLAGRRVTLSLALEAPQPRVLGFWGTAVVRRSSDPVA
                     QGTPPARPHGVIVMLADTLRADHLDRWGYGRETSPQLSELARDGVTASDCISQATWTK
                     VSVPSIFTSLYPRTHTVAEFTDRLPASADTLAEQFRRAGHATLALTSIPFAGQFTNLH
                     QGYEELHESSSLLDSPRAKTAREYVDRLLPWLDEHRDVPFFVFLHVADPHSPYRVDPP
                     YDTWWGDAGDADRLEQMQEQVRPLIDNATMRRFGMPTRSELTEAGIDPERYVDHELDA
                     YDGSIRAMDVELGRLLERLAELGLADRTVLAFVSDHGTEFLDHDAHFHGHTVYGELNR
                     VPLLLWGPSFLPRGVVLEPTVQTIDLMPTLLELAGLESPARAQGRSLLPLISDAERGE
                     PQRWPHPAITEKARLETRNLPGRRYESMSLIADGWKLVRHRPAPAGIDEFELYDHRRD
                     PLNLDNVASRHPERLRELDLMLRAWESSVLAARLDDAALDHDLDPQELERLRSLGYLQ
                     "
     gene            complement(8977..11052)
                     /locus_tag="VD788_12810"
     CDS             complement(8977..11052)
                     /locus_tag="VD788_12810"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXV77191.1"
                     /translation="MTALNPEQRSAVEHADGPLLILAGAGSGKTRVLTHRIARLVDRG
                     FDPGSIVAVSFTNKAADEMRRRTASLVGSRRASLLTMSTFHSFGLTLLREELRESGDR
                     ARFVVFDQADSLGLVREILRGIYRGGAARRFDPLAILTRISNWKSASVRPADAAADSA
                     CEYDAVAREIYPEYDERLQAMRAYDFDDLVCLPVRLLRDDPALRERWRGRIAHLLVDE
                     FQDTSRVQLELVKLLANAQRNVCVVGDDDQSIYGWRGADVGNILQFATHFPGARVIKL
                     ETNYRSRAPILDVANAVIAGGAHRHSKTLLAARGAGDAVRICLCDDGQAEASFVAGEI
                     RDLTSAGSYRLDQIAVLYRSNLQARPVEQALTEAGIPYRLFGGQQFLDRKEVKDVAAY
                     LRVVVNRSDEISLRRILNYPARGVGGRSVRRLERHAREHGIPFVDAFDRADAIVDLPD
                     GARHGLASLRALLGEARRRLERTGRLSAVAHRLIEEIDLRAALDDRAEGGNQGEARFH
                     NATALVGWIERYERETPRDRKSLAEFLQRVALGGARADQDEDRPGVTLSTLHAAKGLE
                     FPVVLLISCVEGRLPHSRSTDPTKSAAVAADPDEERRLFYVGITRARDRLYLLATRQR
                     TLRGKTVDVAPSRYLDGIPPDRLERYERPEQRELSGSELSAAVRELLDRHRGPGGRAA
                     EPGRDGYCR"
     gene            11500..12096
                     /locus_tag="VD788_12815"
     CDS             11500..12096
                     /locus_tag="VD788_12815"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXV77192.1"
                     /translation="MLLYDNPLSGNCYKVRLLLAQLGMRYERRELSVADRSNRSRVLG
                     GLNPALRVPTLVLDDGRPLGESNAILWYLGQGTRYLPDDRYQRAQMLQWQFFEQYDHE
                     PNVAVARFWLQHRAADLDPQALAAKQTAGYRALDAMERHLNDRRFLVGERYTLADISL
                     YAYTHVAEEGGFELSRYPAIRAWLARVAAEPGHVAIDA"
     gene            complement(12150..14429)
                     /gene="aceA"
                     /locus_tag="VD788_12820"
     CDS             complement(12150..14429)
                     /gene="aceA"
                     /locus_tag="VD788_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase ICL2"
                     /protein_id="HXV77193.1"
                     /translation="MSKMTTTTSAFEQEIHETQQWFDGPRFDGILRLYSARQVVEQRG
                     TIRQDYSVARSTAEAFYDRLRELFRARRSITTFGPYSPGQAVALKRMGIEGIYLGGWA
                     TSAKGSDSEDPGPDLASYPLSQVPNEAATLVRALLTADRNQHYLRSRMTDAQRASTRP
                     HDFRPFIIADADTGHGGDPHVRNLIRRFVEAGVPGYHIEDQRPGTKKCGHQGGKVLVA
                     EDEQIKRLNAARFQLDVMKVPGIIVARTDAEAATLLDGRGDERDQPFLLGATNVNLPS
                     YKTCLLAMLKRFHDAGIDEIRGHLLYALSKEEYEEASAWLKHKDLLALIDRKAAEYRT
                     GDHPSIDSVLDEVETRFLEVWETEAGLKTYAQAVADVLEFRASEGELFEISAAEWNEF
                     ARRTSLYRAKQRAKSLGVQVIWDCELAKTPEGYYQMRGGLAYAIAKSLGVAPFADILW
                     METKTANLDDARRFAQAIHAEYPDKMLAYNLSPSFNWDTTGMTDEEMRHFPDELGKLG
                     FVFNFITYGGHQIDGVAGEEFATALRQDGMLALARLQRKIRLVESPYRTPQTLVGGPR
                     LDAALAASSGRTATTKAMGKESTQHQHLVQTEVPTKLLEGWLASWCEHYDLGTTLRVR
                     LRPHRAGSELLELGVYNEADEKVANVVFATIHDRRGRVLLSIRDQNTFEETLRKKRLM
                     TLIHLFLAHRYKADSIHYVSPTDDNRYQTAKMKSHGIFSDVTDEIGQIIVAEVDKERI
                     GQLLEPDGRALGRLITKTD"
     gene            complement(14426..16315)
                     /locus_tag="VD788_12825"
     CDS             complement(14426..16315)
                     /locus_tag="VD788_12825"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77194.1"
                     /translation="MGRAGELQVRDALGERYADVWTPRVVDAIDALAPLDEERRRIMA
                     ERIERRWKRLAGGRPPGFLAPAARIPRTGLTAGQARAGQFDGAEIPPDLERQWIQGTG
                     PGTRPRASVADGLRNVAYALLSGADGWMFDGEDALGQTETMSLDNQRNLKLAIHRDPR
                     FLDAAERVAGEMNDWAERFFGRRIIDDWRKQLDFTTRIFRVRGLHLDDRHVRRAGGAG
                     FSASIVDLALYVVNNHERLRAEGRSVVLYLPKIETAEEAALFNEMLYGLERQLGLAPG
                     TIKAYVLVERIEACFQLMEIRAALAPHFVGFNTGRWDYINSVADAMAGSPAFVNPNID
                     EIGMTYGYMRHYEDRVRRAVNTADRNGRFALWQGGMEPNIPVGSAAGVKAGMARAVAG
                     GERERREGASGKWVAHWKMVHIVRPVWDSVGDDNQLGRAFPELTYRDDDAAGLLALEA
                     APRTVRGARDLLSVALQYGNAFGRGFQAAALKPADFFGDDDVLYLMEDMATGEIRLSI
                     LWEWLHKGAVLTADDPETGVTAGDRFGDELFSRLIDEEYVKLQRAGDRDVHDDSKTTT
                     LPIAREIVARYVREPVKLPWYIDLLNINLNNHDLDLARRRIEGYVDAYHRDGTRITEN
                     LDFEE"
     gene            16529..17407
                     /locus_tag="VD788_12830"
     CDS             16529..17407
                     /locus_tag="VD788_12830"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HXV77195.1"
                     /translation="MTRSWGRLVRVWLAVALAATGLVLAQQDEQAATEEKRWSNKTEL
                     GLTSTSGNADATNLSLGNNYKRNWAHAELLFDIAAIRNESEILSYELNDPLLPPDAPG
                     NAFLVETTETTAETYAAGLTYRHDITKRLFWYVNGTWYRNQPAGIDDRYRGGAGLGYR
                     FIETARHQLLGEVGGTHTDETQTDDETQTYTSARGVVQYDYKISDTAELASDLEGFFD
                     VDDSDNWQSNWITSLTASLNSQLALKVTYAIVYNNRPPNRSIAPGRGTPAGAPSLEFP
                     ADNTDRFLTASVVLNF"
     gene            complement(17416..17491)
                     /locus_tag="VD788_12835"
     tRNA            complement(17416..17491)
                     /locus_tag="VD788_12835"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17456..17458),aa:Val,seq:tac)
     gene            complement(17550..18647)
                     /locus_tag="VD788_12840"
     CDS             complement(17550..18647)
                     /locus_tag="VD788_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77196.1"
                     /translation="MWKRIGIAAGLSLVGAAIVFIAGCEDTDVTAPQDSTITVVASPA
                     AITINQETGQTRGSTTIIAQLLSASGIPQSGVPLFFTSTGGLMGSVDNQCSAGNCVMT
                     GESCTLDGDCRAISPTSIETDSNGIATDILTLRLAEDPASVDVTVQSTVSATTVSVAT
                     NVSAGPQAQISASPTNGARTGQPITFTAATQSGVVITCYEWTIASSLSGVPDELIFRT
                     TPTLAKRYGDTYSRSAEQDLQVRLRVSGQTGACGASLPFSQFEDSITYLIRCDFTDPS
                     VDAGPNITRSLANDQNVDGDVVVALTATAFDEEDAELTYQWDCMTGASPPPGATAECK
                     YNTEGTFAPRVRVTNRCGRFVEGNLTVQINP"
     gene            complement(18842..>20549)
                     /locus_tag="VD788_12845"
     CDS             complement(18842..>20549)
                     /locus_tag="VD788_12845"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV77197.1"
                     /translation="NVIAASSGYYKVLDFGVARIEADAVPGGNPTQLATRSWATSAGG
                     LLGTIAYMSPEQALAEPLDARSDIFSMGSLLYELATGRPAFSGNNAIATANAIVHQQP
                     APLRSIRPRIPEGLALVIEKCMAKRPDDRYPTADALVRDLRTLQLSSLSTSRSYRGLL
                     AHRERPLRGRRWPVLAGAALLVLFSVLLSFAIWLRRDGAAPATDGPPSRAPSAATALP
                     ATGVADKPRVIVAFFENPTGDPAAAWLVRGLPEMLTTDLSRSDKIEVIATQKLYDLAS
                     MAGGERGVTVDRTTIAELARWAGADLVISGSVFKLGEVYRIDAQAYDTDSGTIRVARK
                     VEGADLLAMATELAAGLREQLRLDEDAPSEPLQAATSSAEAFRLYTAAAEASDHLEFG
                     RAVERLTRSLELDPRFSLARLRLAMSQYELGDVDGATASVRQVVADAERLPEAERMLA
                     EALESLWIRRDLEGGSARLERLIESYPRHRSAYVVWARALDRLGDDPLAATRKLQRAI
                     EQDPNNLLAIVSLAEQMARFGATDDAEAILREAARRNPGAEASLERWIRELPRPAQPG
                     PG"
BASE COUNT         3026 a         7230 c         7184 g         3109 t
ORIGIN      
        1 gatcgcggtc cgacgtgccc gccgcattcg cggccctcga agacgaagcg gcgtgcaccc
       61 tcgtcgagga aggtcgcgag cagtccgggg gacggcacgt ggaggtaggt cgcgatcccg
      121 tcgccctcga gcgccaccgc ctgagacggc cgcccgccgg cgatgagcgc gaagggtggt
      181 cggacgtcgc ggatcacctc gagctgctcg cggcgcagct cgggcgggac gaagccgagg
      241 atgccgacgc cccacggacg ccgcccgaga cgctcgcgcg tctcgtcgag cacccgccgc
      301 gcctccgggc cgcgcatcaa cgccagcgcg aggaacggca gtgcaccgtt gtcggcgacg
      361 gcttcgatga accccgcgcg gtcactgacg cgcgtcatcg gcccctggac gatcggatag
      421 cgtgtgccgt gtgctcgagc gagcgggctc gccggcgcca gcgtctcccg tcgcgcggcc
      481 tcgcgcggca ggcgttcggc cgccgtgcgg aacgcgtcga gcatcgttcc cgtcgtctcg
      541 tagcgttcgg cgagccggcg ggccaaggcg atcccttgac ccaagggtag cggggacgga
      601 ccgtcggccg accggtcgct taagcgatca cggagcccgt cccacgggcg gccgccgcgg
      661 accagttctt cctgggcggc ggtgatcgtc tcgcgatcgc gctgccgaaa gaggcgcagg
      721 tggatctcac cgtcgtcgag cgcgatcgac tcggttccgt cgaggcgagc gaacaacgct
      781 cgatcggcct cggcgaacgg cgactcgcgc gcgagccaca gctcctcggc cagcacgacg
      841 ccgtcggcgc ccgcgacgcg cgccgcgccg accgtgtcga cgccccacgc accctggacc
      901 cagaccggcg cacccgctcc gccgcgcagg ctctgcagca ggaggaagct cgagtccgag
      961 ccgacgcggc cacctgcttc gttccccttg agcacgacgc cgtcgtagcc ggcgccggcc
     1021 gccgcgcggg cggcgtccgc atcgagcgcc tcgaacagca cgcgctccgc ccgctcgcgg
     1081 gcccagacca accgctcggc caggccggcc gggtcgaccc cggcgagcag caggatcggc
     1141 agccggccga ccgatgccgc gcggtcgaaa gcggcgacgc tccgccgggc gtggacccac
     1201 ccatcccagc gcacgcccca gccgctgccc cgcggggcct ggcgcgccag ccgcgccagc
     1261 gcgacacgcc aggcggtcgc ggactggccg ggaccgaggt tcagcagccc gatctcgccg
     1321 gctcgcgccg cggcgatcgc gaggtccggg tcggacaggt gctcgggtgt gcagacaatg
     1381 atcgaaaaag ccaagcgggt gttgcccttc agtcgtccgg agcctgctgg aacgaggctc
     1441 cgctcgagtc cgtgattata ccgaaaggcc gggctgcggc gggtctcagc cggtcggctc
     1501 gacgccgtcg gctcgccgct cgtccgggct gtcggccgta cgtgttcgca gcagcagcag
     1561 ttcgtcgcca aacgccaccg gacgccactc ggcggggttc agcatccgca gcgacgcccg
     1621 gtcgaattgc gcggagtcga cgagcaacga gttgatcagg atcgcgtcgg gatcgtggtc
     1681 ggcgacgagc gagcccagcg tgacgcgcac gccggcgccg gcgccgcccg cgacggtcgc
     1741 gaacggctcg atcgccgtgc atcgccggcg gccgagatag ttggcgtagg agctcgcgcc
     1801 gacaccgacg agcgtgtgcc gcgagtcggg ccacagccgc tcgagtcgcg cgaccttgtc
     1861 gagaaccgga cgcacgcggg acggtcggtc gtaaggtcgg ccggcggcga gcgtcagccc
     1921 ggcgacgccc agggtcagga cgaccgtgcc ggcggccagc acacggcgca acgaacgcga
     1981 ggaaacggtc tcccaggacg ccgacacgag gacgaccagc gtcagcccgg ccagcgggac
     2041 cagcgggaag aggtagatcg gcttgggccg cacgaccagg cagggcagcg tcgcgagagc
     2101 cccgagcccg gcgatcaacc cacggtcccc gagcatccgc gcgcgggcgg cgaccgcgga
     2161 tgacaaccgt cgcagactgc cgagcgccac gacgaccggc agcagcagcc agcacgcaac
     2221 cagcacccat gccgggcgca ggcggtcgaa atgaagcggc gtcgcgagcg accgtgccgc
     2281 gccgggcatt cgtcgcaggt tcgttgccgt gtgccgggcg aaggccgccg ggttggcccg
     2341 ggcggcctcg gccgtcgacg tcgaaccgcc gaagtcgcgg cgcacgatca agtgatagtc
     2401 gacccacggg ttgaccgagg ttcgtccggc ttcacgctcg gcgaccgcat agtgctggcc
     2461 gaaggcgtgc cacgctcgct tgccgccgac gtcccaaccg tcgacgtgca cggcggtgta
     2521 cgccgcgagg gcgagcagca cgccgcacgc cgcgagcccg agcgatcgcg gccggccggc
     2581 cggcgcgcgc ccgcctcggc gccaccggcg cacggcgaga gctccgccgt aggccaccag
     2641 gggcagcaag aactcgaggc gcgcgagcga gcacagcgcg agcagcacga gggccaacgg
     2701 cggatggtcg cggccggccg ccgtcgccgc gatgaacagc gcgagaccga agtggtagac
     2761 gagcagcgat ccgtcgaggt tcggcacggt cgcgacccac agcacggcga cccacagcgg
     2821 aggcgcgacg tgcgggctcg agcggtcgag catcagccac aaggcgagcg cgacgagcac
     2881 cgtcaccagg tacttcatcg cgaagaccga cgcgacccgg ccgaacgccc cggccagcac
     2941 ggcataggcc aggttgtaga gcggcgacgc cgccagctcg ccgagcgtgc cgctgccctc
     3001 ggcgaagtcg gcgccccacg ccatctggtt cgactcgtcc cagaccggca ggtccatcgt
     3061 ctcgtgcagt ggatcgagca agacgagctg gaacgagacg acgacggcga cgccgcagat
     3121 cgtcccgaag gtcgcgttcg gcgtcatcgg ccctcctccg acgagggacc gggcggggcc
     3181 tcatcttgcg ccagaacggc gacggaagtc gatcgttttc gcggaaacga gcgaaaccgg
     3241 ggagcctcaa gcggtgttgc gatcgagtcg ttcgagcagc tcgagcgcca gtcgtttcgt
     3301 ccgcttcagc ccgctgcggt agtcgaaact gtcggtgtcg gcgatctcca taccgagatc
     3361 ggagacctcg ctcgccagga tcctgcgcag caccgcggct tgctcgtccg tcaaccggag
     3421 ctcgatcatc gccgcgcctc ccttcggatt ataggtcggt cgcgccgcgg gcgagtcgac
     3481 cgttctcgtc cgaattcgga acgtcgcgtc cgttgaacct tcaccgccgc cgcgagtaac
     3541 ttccccggcg gacccgtcgt ggtcgggggg agccgatgcg atcgagcgca atcgacaacg
     3601 aggacgtccg gcggctgcgc ggtcttcgcc acctcgtcgg caacgcgccg atcctggccg
     3661 tcgagctgac ctatcgcggc accccgcggc ggatctacgc caaggcggag cagctcaacc
     3721 tgaccgggag catcaaggac cgcatggcgc tccacatcgt gcgcgagggg cggcgtcgcg
     3781 gtctgctcgc ggcgggggcg cggctcgtcg aagcgacgag cggcaacacc ggcatctcgt
     3841 tcgcggccgt cggccgggcg atgggacacc cggtcacgat cttcatgccc gactggatga
     3901 gtcgggagcg cgtcgagctg atccgcagcc tcggcgccga gatccggctg gtgagccgcg
     3961 cgcagggcgg tttcgtcggc gcgatcgagc tggccgagag ctacgcccga cgaacgccgg
     4021 gcgcgttcct gccgcgacag ttctccaacc gcgacaacgt cgccgcgcat cgcgagacga
     4081 ccggacccga gatctggtgg cagatgcgaa accacggcct gacgcccgat gtcttcgtcg
     4141 ccggcgtcgg tacgggcggc acgatcatgg gtgtcgggca ggcgctgcgc gagttgagcc
     4201 cgacggtcaa gctccacccg ctcgagccgg cgagctcgcc gacgctgcgc accggtcact
     4261 gcgtcggcag gcaccggatc cagggcgtgt cggacgagtt catcccgccg atcgtcgatc
     4321 tcgacttcct cgacgaagtc gtcgacgtcg aagacggtga cgcgatcctg atggcgcaga
     4381 aactcgcgcg cgagctgggt ctcggcgtcg gcatctcgtc gggcgccaac ttcctcgggg
     4441 cgctcgcgct ggtcgagcgc tacggcggcg aggccaacgt cgtgaccgtc ttccccgacg
     4501 accacaagaa atacctcagc accgatctct tgcgcgagga gccggcgcgc gaaggctatc
     4561 tcagcccgga ggtcgagttg ctccgcttca gcgccttcaa gcgggtctgt cgcacctgct
     4621 gcgagccgtc cgattgcctc gtgccccaga cgtcggcggg cgaagacggc gtcttgccgg
     4681 gctgcccgct gtggcgcgac cgggtggaat ccccggtttg aaccgcccgg cgccgatcgc
     4741 ggcgccggcg ctggcccgca gtcgtcgccc gaccgacatt cgccgacgcg gtgcgaagaa
     4801 aggcgacccc gcgggtcgcg aatcggtatg atacgcaacc cggcggcgcc agtcgtcacg
     4861 cttcgacatg gagatccgat ggaagctctc gaacgcgcgg tcgcggccgt tcgcgacctg
     4921 atttggaagt tcggcctccc ggtcggcgac gagacgatcc cgctggtggt gatcgcgctg
     4981 ctcgggaccg gggtgttcct gacgctgcgc ctgggcttcg tccaggtccg gcggctcggg
     5041 cacgggttcg cggtcgcgac cggcaagtac gacgatcccg acgagccggg cgacgtctcc
     5101 cacttccagg cgctggcgac cgcgctgtcg gcgaccgtcg gtatcggcaa catcgccggt
     5161 gtcgcgatcg cgatccactg gggagggccg ggcgcgctgt tctggatgtg ggtcaccgcg
     5221 gcgctcggca tggccgtcaa atacaccgag gtgacgttgg cccaacaaca tcgcgacgtc
     5281 gggcccgacc tcgacgccaa ccggtgggag ggctcggtct ccggcgggcc gatgtactac
     5341 atcgagcgtg gtctcggacc ggcgtggaag ccgatggcgg tgttcttcgc ggcgatgctc
     5401 ggcagcacgg ccttcctcac cggcaacgcg atccaggcca acaccgtcgc cgacacgctc
     5461 gagacgtcga tcggcatgcc ggtctgggtc accggcgtca tcaccgccgg cgtggtcgcc
     5521 gcggtcatcc tcggtggaat cacgcggatc gggcgcgtga ccggcgtcct cgcgccgctg
     5581 atggccgcga tctacgtcag cggggcgctg ttgattctgc tgctcaacgt cggcgccgtc
     5641 gggccggcgt tcctgtcgat cttcaccgag gcgttcaatc cgtcggcggg cgtggccggg
     5701 accgggatcg gcgcgatcct ggtcacgatg atgtggggcg tcaaccgcgg tctgttctcg
     5761 aacgaggcgg gccagggctc ggcaccgatc gcgcacgccg ccgccaagac cgacgagccg
     5821 gtgtcggagg gtgtcgtcgc gctgaccgag ccgttcatcg acacgatcgt gatctgcacg
     5881 ttcaccgcga tggcgatcat cgtcaccggc gtctacgacg accgcgtgcc gacgacgatc
     5941 atgctgagcg gcggcgacat cacctacgtc gccgtcgacg agcggggtct gaggtcggaa
     6001 gtcgccgcgc cggccgagat tcgtgtcgag gacgggctgc agctcgtcga tccgacggcg
     6061 ccacggctgg cgtggcacga cgtgccggtc gagcggctgg cggtcgacgc cgaccggcgg
     6121 gtcggcttca ccggcgtgat ccggccgtcg gcccgcattg cccgcggcga cgacggcgtc
     6181 gagtacaccg cgttgtacgc cgatgcggtc gagagcgggg ccccgctgac gctgctcgcg
     6241 ttcgaccgcg ggctcgcccc gatcggcggc ttcggtccgc acgtcgtgac cttgtcggtg
     6301 ttgctgttcg ggatctcgac ggcgatcgcg tggagctact acggcgaccg ctgcgcgacc
     6361 tatctgttgg gtcccaaggc cgtgctgccg tacaagatgg tctacgtgtt gatgcacttc
     6421 ctcggcgccg gcgtgagctt gcagctcgcc tgggacttcg gcgacgtctt cctcggcatc
     6481 gtcatcctgc ccaatctgat cgcgctcgtg ctgctgtccg ggcgggtcaa ggagatgacc
     6541 gacagctact tcgagcggcg gccctggatc gagaacgccg aggcgcacaa gcgtgccgtc
     6601 gcggcgcggc ggaccggctg agccggtgcg cggtctcgcg gcgatcgcgg tcggtgcgct
     6661 gctcgtcgcg ggctgcaccg ggggcgagcg cccgacgccg ggcgtcgtcc ggctggtcga
     6721 tcggttcgcc gccgcgtcga tcgacgggtc gccgccggcc gccgcgccgg ggacggtcgg
     6781 gccgggctgg acgttcgagg gcgccgcggc cgccggcgga cccgccgcgg gttggcagac
     6841 gcaccgcgtc gacggcttcg ccgtccgcga cggtctgctg cacggtcgcc cgcgggacga
     6901 aacgtcgctg atccacgccg cggtcgacga gcccgccgac ggcgccgacc tgctgcacgc
     6961 agtcgagatc gcgatccgga gcgaggccgg cggcgagctt gcgatcggtt tcagcgagga
     7021 cgacgagctc gacctcgacg aggtgctgga caacccgttt ccgtggccgc tccgcacgcc
     7081 gctcgtcgcg gcgccgaccg tcgagcgcta ctcgctgcgc ccgacgcgcg cgacccatct
     7141 gtcgagcctg cgccacctgc tgatccgccc gtcgggcgag ttcgcgatcg aatcggtgcg
     7201 cgtcgtcagc cgccgggagc atctggccgc gatcgaatcc ggcgtcggtt ggcacggaat
     7261 ggaagatgtg ttccgtgagg cgatcgtcac tcgctcgccc gaggtcgcac ggttcgagat
     7321 cgacctaccc gagcgacctt ggctcgatct cgccgtggcc acgatcgagg agggtgcggc
     7381 gaccttccgc gtcgaggctc ggtccgcagg cgagccgccc atcgtcttcg agcgcaccgt
     7441 cagccgcccg caccgctgga gcccggtgcg gatcgacctc gcgccgcttg cgggacggcg
     7501 ggtgacgctg tcgctcgcgc tcgaggcgcc acagccccgc gttctcggtt tctggggcac
     7561 ggccgtcgtg cggcgctcga gcgacccggt cgcgcagggg acgccgccgg cccggcccca
     7621 cggcgtgatc gtgatgctgg ccgacacgct gcgtgccgat cacctcgacc gctggggtta
     7681 cgggcgggag acgagcccgc agctgtccga actggcccgg gacggcgtga cggcgagcga
     7741 ctgcatctcg caggcgacct ggaccaaggt ctcggttccg tcgatcttca cctcgctcta
     7801 cccgcgcacc cacacggtcg cggaattcac cgaccggctg ccggccagtg ccgacacgct
     7861 ggccgagcag ttccgccggg cgggtcatgc gacgctggcc ctcacctcga tcccgttcgc
     7921 cgggcagttc accaacctgc accagggcta cgaggagttg cacgagtcga gctcgctgtt
     7981 ggactcgccc cgcgcgaaga cggcgcggga gtacgtcgac cgcctactgc cgtggctcga
     8041 cgagcaccgc gacgtgccgt tcttcgtctt cctccacgtc gccgacccgc acagtccgta
     8101 ccgcgtcgat ccgccgtacg acacctggtg gggtgatgcc ggcgacgccg accggctgga
     8161 gcagatgcag gagcaggtac ggccgctgat cgacaacgcg acgatgcggc gcttcggcat
     8221 gccgacgcgc agcgagctga cggaagcggg catcgatccc gagcgctatg tcgaccacga
     8281 gctcgacgcc tacgacggtt cgatccgggc gatggacgtc gagctcggga ggttgctcga
     8341 gcgcttggcc gagctcgggt tggccgaccg cacggtgctg gcgttcgtca gcgatcacgg
     8401 caccgagttc ctcgaccacg acgcgcactt tcacggccac accgtctacg gcgagctgaa
     8461 ccgggtcccg ttgctgttgt gggggccgtc gttcctgcca cgtggcgtcg tgctcgagcc
     8521 gacggtgcag acgatcgacc tgatgccgac gctgctcgag ctggcggggc tcgagtcgcc
     8581 cgcgcgggcc cagggccgca gcctgctgcc gcttatctcg gacgccgagc ggggcgagcc
     8641 gcagcgatgg ccccacccgg cgatcacgga gaaggcacgg ctcgagacgc gcaacctgcc
     8701 cggccggcgc tacgagtcga tgtcgttgat cgccgacggc tggaagctcg tgcgccaccg
     8761 gccggctccc gcggggatcg acgagttcga actgtacgac catcgtcggg acccgctcaa
     8821 cctcgacaac gtcgcctcgc gccaccccga gcggctgcgc gagctcgacc tcatgttacg
     8881 cgcgtgggag tcgagcgtgc tcgccgcgcg gctcgacgat gccgccctgg accacgacct
     8941 cgacccgcag gagctcgagc gtctgcgcag cctcggctac ctgcagtaac cgtcgcggcc
     9001 cggctcggcc gcccgtccgc ccggtccgcg gtggcgatcg agcagctcgc gcaccgcggc
     9061 cgacagctcg ctcccgctca actcgcgttg ctcgggccgc tcgtagcgct cgagccgatc
     9121 cggcgggatc ccgtcgaggt agcgcgacgg cgcaacgtcg accgtcttgc cgcgcagcgt
     9181 ccgctggcgc gtcgccagca ggtagagtcg atcgcgggcg cgtgtgatgc cgacgtagaa
     9241 cagccggcgc tcttcgtcgg gatcggccgc gacggcggcg cttttcgtcg ggtcggtcga
     9301 tcggctgtgc ggcagccgtc cctcgacgca gctgatcagc aggacgaccg ggaactcgag
     9361 gcccttggct gcgtgcaagg tcgacagcgt gacgccgggg cggtcctcgt cttggtcggc
     9421 gcgcgcgccg ccgagcgcga cacgctggag gaactcggcc agcgacttgc gatcccgcgg
     9481 agtttcccgc tcgtagcgct cgatccagcc gaccagcgcc gtcgcgttgt ggaagcgcgc
     9541 ctcgccttga ttgccgcctt ccgcgcggtc gtcgagcgcc gcccgcaggt cgatctcctc
     9601 gatcagccgg tgcgcgacgg cgctgagccg gccggtgcgc tccagccggc ggcgtgcctc
     9661 gccgagcagc gcgcgcaggc tggccaaccc gtgccgcgct ccgtccggca ggtcgacgat
     9721 cgcgtcggca cggtcgaacg cgtcgacgaa cgggatgccg tgctcgcgcg cgtgccgttc
     9781 gagccggcgg accgagcgac cgccgacccc gcgcgccgga tagttgagga tgcgccggag
     9841 cgagatctcg tccgaccggt tgacgacgac ccgcaggtag gcggcgacgt ccttgacttc
     9901 cttgcggtcg aggaactgtt gtccgccgaa gagccggtag gggatgccgg cctcggtcaa
     9961 cgcctgctcg accggccgcg cctgcaggtt ggagcggtag agcaccgcga tctggtccag
    10021 gcggtacgac ccggcgctcg tcagatcgcg gatctcgccg gcgacgaagg acgcctcggc
    10081 ttgcccgtcg tcgcacaggc agatccgcac cgcgtcccca gcgccgcgcg ccgcgagcag
    10141 ggtcttggaa tgccggtgcg cgccgccggc gatcaccgcg ttcgccacgt cgagaatcgg
    10201 cgcccgactg cgatagttcg tctcgagctt gatcacgcgg gcgccgggaa agtgcgtggc
    10261 gaactgcagg atgttgccga cgtcagcccc gcgccagccg tagatcgact ggtcgtcgtc
    10321 gcccacgaca cagacgttgc gctgcgcgtt ggccagcagc ttgaccagct cgagctgaac
    10381 acgactggtg tcctggaact cgtcgaccag caagtgtgcg atccgtcctc gccagcgctc
    10441 gcggagagcg ggatcgtcgc gcaacagccg caccggcagg cagaccagat cgtcgaaatc
    10501 gtaggcccgc atcgcctgca ggcgttcgtc gtattcgggg tagatctctc gcgcgaccgc
    10561 gtcgtactcg caggcggaat cggcagccgc gtcggccggt cggaccgacg cgctcttcca
    10621 gttggagatg cgggtcagga tcgccagcgg gtcgaagcgg cgtgccgccc cgccgcggta
    10681 gatcccgcgg aggatttcgc ggacgagccc cagcgaatcc gcctggtcga agacgacgaa
    10741 gcgggcgcgg tccccgctct cgcgcagttc ctcgcgcagc agcgtcagac cgaagctgtg
    10801 gaacgtcgac atcgtgagaa gcgacgcgcg gcgggagccg acgagcgacg ccgtccgacg
    10861 acgcatctca tcggcagcct tgttggtgaa gctgacggcg acgatcgacc ccgggtcgaa
    10921 gccgcggtcg accagccggg cgatgcggtg ggtcaggacg cgcgtcttgc cgctacccgc
    10981 tccggccagg atcagcagcg gaccgtcggc atgctcgacg gcggaccgct gctcggggtt
    11041 caacgcggtc atcgggcggg atcgtcgtcc ggtgcggcca tcggcgccgc atggtagcaa
    11101 gatcgaccgg cgggatcacg ggtgcaaacg accggccggc gcctcagctc tggatcggac
    11161 cggaggtcgc gcagttgtcg tcggctttca tacagaccgc cgcgcgcgcc gagagcggct
    11221 cgctgtggac gatggcgggg cgttcgtagg gctttttcat cgtcgtttct ccgtgccgtc
    11281 gacgagcgcc gagcggccgg cgatcgtccc gtccgggcgg aatgtagcgc ccgcggcgca
    11341 cgcgtgcacg cggacgacgg ccgcttgcgg tcgcgaccga tgccaaccga ctgtagggaa
    11401 gccagttaga aacgatggcg cgcgggcgat gaggccgatc gactaccgtc actcccggcg
    11461 ttgcgctacc cttcgcccca tccggccgga ggctcgagcg tgctgctcta cgacaatccg
    11521 ctatcgggca actgctacaa ggtgcggctc ctgctcgcgc agctcggaat gcgttacgag
    11581 cgccgcgagc tgtcggtcgc ggaccggtcg aaccgctccc gggtgctcgg cggcctcaac
    11641 ccggcgctgc gcgtgccgac cctcgtgctc gacgacggcc gaccgctcgg cgagtcgaac
    11701 gcgatcctgt ggtacctggg ccaggggacg cgctacctcc ccgacgatcg ctaccagcgc
    11761 gcgcagatgc tgcagtggca gttcttcgag cagtacgacc acgagccgaa cgtcgccgtc
    11821 gctcgcttct ggctccagca ccgcgccgcc gacctcgacc cgcaggcgct ggcggccaag
    11881 cagacggcgg ggtaccgcgc gctcgacgcg atggagcgac acctcaacga tcgccggttc
    11941 ctcgtcggcg agcgctacac gctggccgac atctcgctgt acgcctacac ccacgtggcg
    12001 gaagagggcg gcttcgagct gtcccgctac ccggcgatcc gcgcctggct cgcacgtgtc
    12061 gccgcggagc ccggccacgt cgcgatcgac gcctgagatc gaccggccct cgaacgacct
    12121 cggaaaaccc ggtcgtgctc gaccaggcgt cagtccgtct tcgtgatcaa gcggccgagc
    12181 gcgcggccgt cgggttcgag caactggccg atccgctcct tgtcgacctc ggcgacgatg
    12241 atctgcccga tctcgtcggt cacgtcgctg aagatgccgt ggctcttcat cttggcggtc
    12301 tggtaacggt tgtcgtcggt gggactgacg tagtggatcg aatcggcctt gtagcggtgg
    12361 gcgaggaaca ggtggatcaa cgtcatcagg cgtttcttgc gcagcgtctc ctcgaacgtg
    12421 ttctggtcgc gaatcgacag cagcacccgg ccgcgccggt cgtgaatcgt cgcgaagacc
    12481 acgttcgcca ccttctcgtc ggcctcgttg tagacgccga gctcgagcag ctcggaaccc
    12541 gcccggtgcg ggcgcagcct gacgcgcaac gtcgtgccga ggtcgtagtg ctcgcaccac
    12601 gacgccagcc aaccttcgag aagcttggtc ggcacctcgg tctgcacgag gtgctggtgt
    12661 tgggtcgact ccttgcccat cgccttcgtc gtcgcggtgc gaccggacga ggcggcgagc
    12721 gccgcgtcga gccgcgggcc gccgacgagc gtttgcggcg tgcgataggg tgactcgacc
    12781 agacggatct tgcgctgcag cctcgccagc gcgagcatgc cgtcctgcct cagcgcggtc
    12841 gcgaactcct cgccggccac gccgtcgatc tggtgaccgc cgtaggtgat gaagttgaag
    12901 acgaagccga gcttgcccag ctcgtcgggg aaatggcgca tctcctcgtc cgtcattccg
    12961 gtcgtgtccc agttgaacga gggtgacagg ttgtaggcca gcatcttgtc gggatactcg
    13021 gcgtggatcg cctgggcgaa ccggcgcgca tcgtcgaggt tggcggtctt cgtctccatc
    13081 cacaggatat cggcgaacgg cgcgacgccg agcgacttgg cgattgcgta ggccaggcct
    13141 ccgcgcatct ggtagtagcc ctccggggtc ttggccagct cgcagtccca gatcacctgg
    13201 accccgagcg acttggcccg ctgcttcgcg cggtagagcg aggtcctccg ggcgaactcg
    13261 ttccactcgg cggcgctgat ctcgaacagc tcgccctcgc tggcgcggaa ctcgagcacg
    13321 tcggccaccg cctgcgcgta ggtcttcagg ccggcctcgg tttcccagac ctcgagaaac
    13381 cgtgtctcga cctcgtccag cacgctgtcg atcgacggat ggtcgcccgt gcgatactcc
    13441 gcggccttgc ggtcgatcag cgccagcagg tccttgtgct tgagccaggc cgaggcctcc
    13501 tcgtactcct ctttggacag cgcgtagagc aggtgcccgc gaatctcgtc gatgccggcg
    13561 tcgtggaagc gcttgagcat ggcgagcaag caggtcttgt aggacggcag gttgacgttg
    13621 gtcgcgccga gcaggaacgg ctggtcgcgt tcgtcgccgc gcccgtcgag cagcgtcgcc
    13681 gcctccgcgt cggtgcgggc gacgatgatc ccggggacct tcatcacgtc gagctggaag
    13741 cgtgccgcgt tcaagcgctt gatctgctcg tcttcggcga cgagcacctt gccaccctgg
    13801 tggccgcact ttttcgtccc gggccgttgg tcttcgatgt ggtagcccgg cacgccggcc
    13861 tcgacgaacc gccggatcag gttgcgcacg tgcgggtcgc cgccgtgccc ggtgtcggcg
    13921 tcggcgatga tgaacggtcg gaagtcgtgg ggccgcgtcg acgcgcgctg ggcgtcggtc
    13981 atccgcgagc gcaggtagtg ctggttgcgg tcggcggtca gcaacgcccg aaccagcgtg
    14041 gcggcctcgt tcggcacctg gctcagcgga taactggcca ggtcgggccc cgggtcttcg
    14101 ctgtccgagc ccttggccga ggtcgcccag ccgccgagat agatcccctc gatgcccatt
    14161 cgcttgagcg cgacggcctg acccggagag taggggccga aggtggtgat gctccgccgc
    14221 gcccgaaaca gctcgcgcag ccgatcgtag aacgcctcgg cagtgctgcg cgcgacgctg
    14281 tagtcctgac ggatcgtccc gcgctgctcg accacctgtc gcgccgagta gaggcggagg
    14341 atgccgtcga accgcgggcc gtcgaaccac tgctgcgtct cgtggatttc ctgctcgaac
    14401 gcggaggtcg tcgtcgtcat cttgctcatt cctcgaagtc gagattctcg gtgatccggg
    14461 tcccgtcgcg gtgataggcg tccacgtagc cctcgatccg ccggcgggcg aggtcgaggt
    14521 cgtggttgtt cagattgatg ttcagcagat cgatgtacca cgggagcttg accggctcgc
    14581 ggacgtagcg ggcgacgatc tcccgcgcga tcggcagggt ggtcgtcttc gagtcgtcgt
    14641 gcacgtcccg gtcgccggcc cgctgcagct tgacgtattc ctcgtcgatc agccgcgaga
    14701 acagctcgtc accgaagcgg tcgccggccg tcacaccggt ctcggggtcg tccgccgtca
    14761 gcaccgcgcc cttgtgcaac cactcccaga ggatgctcag ccggatctcg cccgtcgcca
    14821 tgtcctccat caggtacaac acgtcgtcgt cgccgaagaa gtcggccggt ttcaacgcgg
    14881 ccgcctgaaa cccgcgtccg aaggcgttgc cgtactgcag cgcgacgctg agcaagtccc
    14941 gcgcgccgcg caccgtgcgc ggtgcggcct cgagcgccaa cagcccggcc gcgtcgtcgt
    15001 cgcgataggt caactcgggg aaggcgcgac cgagttggtt gtcgtccccc acgctgtccc
    15061 ataccggccg cacgatgtgc accatcttcc agtgggcgac ccacttgccg ctcgcgcctt
    15121 cccgccgctc gcgctcgccg cccgcgaccg cacgagccat gccggccttc actccggcgg
    15181 ccgagccgac cgggatgttc ggctccatcc cgccctgcca cagcgcgaaa cgcccgttgc
    15241 ggtcggcggt gttgaccgcg cgacgcacgc gatcctcgta gtggcgcatg tagccgtagg
    15301 tcatcccgat ctcgtcgatg ttcgggttga cgaacgcggg gctcccggcc atcgcgtcgg
    15361 ccacgctgtt gatgtagtcc cagcgcccgg tgttgaaccc gacgaagtgc ggcgcgaggg
    15421 cggcgcggat ctccatcaac tggaagcagg cctcgatgcg ctcgaccagc acgtaggcct
    15481 tgatcgttcc cggggccaga ccgagctgtc gctcgaggcc gtacagcatc tcgttgaaca
    15541 gcgccgcctc ctccgcggtc tcgatcttcg gcaggtagag cacgacggag cggccctcgg
    15601 cgcgcagccg ttcgtggttg ttgacgacgt acagcgcgag gtcgacgatc gaagccgaaa
    15661 agcctgcgcc gccggcccgc cgaacgtgcc ggtcgtcgag atgcaacccg cgcacgcgaa
    15721 agatgcgagt ggtgaagtcg agctgcttgc gccagtcgtc gatgatccgg cggccgaaga
    15781 accgctcggc ccagtcgttc atctcgccgg cgacccgctc ggccgcgtcg aggaaccgtg
    15841 gatcgcggtg gatcgcgagc ttcagattgc gctggttgtc gagcgacatc gtctcggtct
    15901 gtccgagcgc gtcctcgccg tcgaacatcc agccgtcggc gccggagagc agcgcgtacg
    15961 ccacgttgcg gagcccgtcg gcgacgctcg cccgtggccg ggtcccgggt ccggttccct
    16021 ggatccactg gcgctcgagg tcggggggga tctccgcgcc atcgaactgc ccggcacggg
    16081 cctggcccgc ggtcaacccg gtacgcggga tgcgggccgc gggcgcgagg aaccccggcg
    16141 gccggccgcc cgcgagccgc ttccaccggc gctcgatgcg ttccgccatg atccgccgtc
    16201 gctcctcgtc gagtggcgcc aacgcgtcga tcgcgtcgac gacacgcggc gtccagacgt
    16261 cggcgtaacg ctcgccgagc gcgtcacgca cctgcagttc ccccgctcga cccatcgtcg
    16321 ctcctcggcg gcattctagc gagccgccgc tcgcatttct ccccggcggg agaaggccgg
    16381 ggaccgctaa tgtagtgctc cggcccggtg ctgtccgccg ccggggccgc cgctcgtcgc
    16441 gccggttcgt gactgcggga gcgaggctgt acaatcggcc ctttcgttcg cactggaaat
    16501 ccgctcgacc acaacggggg agaggggcat gacacgatcg tggggtaggc tggtccgggt
    16561 gtggctcgcg gtggcgctcg ccgccaccgg gctggtgctg gcgcaacaag acgaacaggc
    16621 cgcgaccgag gaaaagcgct ggtcgaacaa gaccgagctc ggactcactt cgaccagcgg
    16681 caacgccgac gcgaccaacc tgtcgctcgg caacaactac aagcggaact gggcccacgc
    16741 cgagctgttg ttcgacatcg cggcgattcg caacgagtcg gagatcctga gctacgagct
    16801 gaacgacccg ttgctcccgc cggacgcacc gggcaacgcg ttcctcgtcg agaccaccga
    16861 gaccaccgcc gagacgtacg cggccgggct gacctaccgg cacgacatca ccaagcgact
    16921 gttctggtac gtcaacggca cgtggtaccg caaccagccg gccggcatcg acgaccggta
    16981 ccgcggcggc gcgggtctcg gttacaggtt catcgaaacc gcccggcacc agctgctcgg
    17041 cgaggtcggc ggcacccaca ccgacgagac gcagaccgac gacgagaccc agacctacac
    17101 cagcgcccgc ggtgtggtgc agtacgacta caagatctcg gacacggccg aactggcgtc
    17161 ggatctcgag gggttcttcg acgtcgatga ttcggacaac tggcagtcga actggatcac
    17221 gtcgctcacc gccagcctga actcgcagct cgcgctgaag gtgacctacg cgatcgtcta
    17281 caacaaccgg ccgccgaatc ggtcgatcgc gcccgggcgc gggacaccgg ccggcgcgcc
    17341 gagcctcgag ttcccggcgg acaacaccga ccggttcctg accgcgtccg tcgtgctcaa
    17401 tttctgagcc cgggttggta ggcgcgggac ggttcgaacg tccgaccccc accgtgtaag
    17461 ggtggtgctc tgccagctga gctacgcgcc ttcgttgcgt gtaacgccgc ggagcgctcg
    17521 ccgcccggtc gtcccggccg gcgcgcgtcc tacgggttga tctgaacggt caggttgccc
    17581 tcgacgaagc gaccgcagcg gttggtgacc cgcacccgcg gcgcgaacgt gccctcggtg
    17641 ttgtacttgc actcggcggt ggcgcccggc ggcggcgacg ccccggtcat gcagtcccac
    17701 tgataggtca gctcggcgtc ttcctcgtcg aacgccgtcg ccgtcagtgc gacgaccacg
    17761 tccccgtcga cgttctggtc gttggccagc gagcgggtga tgttgggccc ggcgtcgacg
    17821 gacggatcgg tgaaatcgca gcggatcaag taggtgatcg agtcctcgaa ctggctgaac
    17881 ggcaggctcg caccgcacgc acccgtttgc ccgctgacgc gcagccggac ctgcagatcc
    17941 tgctcggccg atcgcgagta ggtgtcgccg tagcgcttgg cgagcgtggg cgtcgtgcgg
    18001 aagatcagct cgtcggggac gcccgacagg ctcgacgcga tcgtccactc gtagcaggtg
    18061 atgacgacgc ccgattgggt cgccgcggtg aaggtgatcg gttggccggt acgcgcgccg
    18121 ttggtcggcg aagccgagat ctgcgcctgg ggcccggcgc tgacgttcgt ggcgacggag
    18181 accgtcgtcg cgctgaccgt cgattggacg gtcacgtcga ccgaggccgg gtcctcggcg
    18241 agacgcaggg tcaggatgtc ggtggcgatg ccgttcgagt cggtctcgat cgaggtcggc
    18301 gagatcgcgc ggcagtcgcc gtccagcgtg caggactcac cggtcatcac gcaattcccg
    18361 gcactgcact ggttgtcgac cgagcccatc aggccgccgg tgctggtgaa gaagagcgga
    18421 acgccggact gcgggatgcc gctcgcgctc agcagctggg cgatgatcgt cgtgctgccg
    18481 cgggtctgac cggtttcctg gttgatcgtg atcgcggccg ggctggccac gacggtgatg
    18541 gtgctgtcct ggggcgccgt cacgtcggtg tcttcacagc ccgcgatgaa cacgatcgcc
    18601 gcgccgacga gcgagagacc cgccgcgatg cctatgcgct tccacacgac ccgtactcct
    18661 cgctgccccg aatcgaccgg gggaacccgc acgaaatacg tcgccgcgct ccccggcgtc
    18721 tagccagtta gccaatatac gggctttctc gccgggcgtc gtcccccgac gcgacgcgcc
    18781 gccgaccccg accgacggcg aggcgctatc ttcaatggat cgagcgcgtt cgccccgcgg
    18841 gtcagccggg cccgggttga gccggccgcg gcagctcccg gatccaccgc tcgagcgacg
    18901 cctcggcccc ggggtttcgt cgggccgcct cgcgcagaat cgcctccgcg tcgtccgtcg
    18961 cgccgaagcg cgccatctgc tcggcgagcg agacgatcgc cagcaggttg ttcgggtcct
    19021 gctcgatcgc gcgctgcagc tttcgcgttg cggcaagggg gtcatcgccc agacggtcga
    19081 gcgcccgggc ccagacgacg taggccgacc ggtgtcgggg gtagctctcg atcagccgct
    19141 cgagccgcgc cgaaccgccc tcgaggtcgc gccggatcca caacgactcg agtgcttccg
    19201 ccagcatccg ttcggcctcg ggcagccgtt ccgcgtccgc gacgacctgg cgcacgctcg
    19261 cggtcgcccc gtcgacgtca cccagctcgt actggctcat cgccagccgc aggcgggcca
    19321 gcgaaaaccg cgggtcgagc tcgagcgagc gcgtcagccg ctcgaccgcc cggccgaact
    19381 cgagatggtc cgaggcctcg gccgccgcgg tgtagagacg gaacgcctcc gccgacgagg
    19441 tcgccgcctg cagcggttcg ctcggtgcgt cttcgtcgag acgcagctgc tcgcgcagcc
    19501 ccgccgcgag ctccgtcgcc atcgccaaca gatccgcgcc ctcgaccttg cgggcgacgc
    19561 ggatcgtgcc gctgtcggtg tcgtaggcct gggcgtcgat gcggtagacc tcgccgagct
    19621 tgaacaccga cccgctgatc acgagatcgg cgccggccca gcgcgccagc tccgcgatcg
    19681 tcgtccggtc gaccgtgacg ccgcgctcgc cgcccgccat cgacgccaag tcgtacagct
    19741 tctgcgtcgc gatgacctcg atcttgtccg accgcgacag atcggtcgtc agcatctcgg
    19801 gcagaccgcg cacgagccat gccgcggccg gatcgcccgt cgggttctcg aagaaggcga
    19861 cgatcacgcg cggcttgtcg gcgacgccgg ttgccggaag cgccgtcgcc gccgagggtg
    19921 cccgggaggg cgggccgtcg gtcgccggag cggccccgtc gcgccgcagc cagatcgcga
    19981 acgacagcag gaccgagaag agcacgagca acgccgcacc ggccagcacg ggccagcgcc
    20041 gccctcgcag cggacgttcg cgatgggcga gcaatccgcg ataggagcgc gaggtcgaca
    20101 aactgctcag ctgcaacgtg cgcaggtcgc gaaccaaggc gtcggccgtc ggatatcgat
    20161 cgtccggtcg cttggccatg cacttttcga tcacgagcgc cagcccctcg gggatcctcg
    20221 gccggatcga gcgcagcgga gcgggctgct ggtggacgat cgcgttggcc gtcgcgatcg
    20281 cgttgttccc gctgaacgcc ggccgacccg tcgccagttc gtacagcagg ctccccatcg
    20341 agaagatgtc cgagcgcgcg tcgagcggct cggcaagcgc ctgctcgggg ctcatgtagg
    20401 cgatcgtgcc cagcaacccg ccggcgctcg tcgcccagga gcgcgtcgcg agttgggtcg
    20461 gattgccgcc ggggacggca tcggcctcga ttcgagccac gccgaaatcg aggaccttgt
    20521 agtagccgga gctggccgcg atgacgttt
//
LOCUS       DASPGN010000441        14908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1015868, whole genome shotgun sequence.
ACCESSION   DASPGN010000441 DASPGN010000000
VERSION     DASPGN010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 14908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14908
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015868"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VD788_12850"
     CDS             complement(<1..362)
                     /locus_tag="VD788_12850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HXV77198.1"
                     /translation="MLRITKEADYGIMLLVAMAERPSGEVQTAREVAERCGLPFPMVS
                     KIMRSLARGEILVSRRGVSGGYSLDRPADTLTVAEIIRAIEGPISMVQCGVEPGVCDQ
                     EPVCPTRLNWARISREVER"
     gene            complement(476..880)
                     /locus_tag="VD788_12855"
     CDS             complement(476..880)
                     /locus_tag="VD788_12855"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV77199.1"
                     /translation="MTTARPSLGSVLETVLYYTDQQRALAFYRDLLGMRLLASEPRRS
                     LFFRAGASVFLLFDADETLAAGTLPAHGARGPVHTCFRVPADDYDRWKRYLPERGVAL
                     LHETRWRNGRSFYFHDPDGNLLEIADNDIWPG"
     gene            964..1896
                     /locus_tag="VD788_12860"
     CDS             964..1896
                     /locus_tag="VD788_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77200.1"
                     /translation="MKMPRAILLGPQRFRPNVAAALDWLGRDGPLALVSAGWQEREDE
                     IDEFVEHCRREVVPLGLYHRAERALARDELLTAALRERQDQLRELQGLYRVRLASLLE
                     AARSLMRRGDGRFVDEHRKAAIRAVRTLDRQHLVRIREIHDEFERRVRPGAHPSVRAE
                     REELQQLVGRCSVIAIAGGHVAVLLNRLRLFDVLGLAADRPVVAWSAGAMALGERVIL
                     FHDSPPQGPGDAEVFEPGLGLYRGLVPLPHASERLALDDPLRVALFSRRFAPASSVTL
                     EYGSRVDWSGDRWVARGEVGLLGRSGRLRRMRPD"
     gene            1902..3029
                     /locus_tag="VD788_12865"
     CDS             1902..3029
                     /locus_tag="VD788_12865"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXV77201.1"
                     /translation="MFERWIADGPPAADELSRRLAEHVFPLVERRSATFAYHGKADDV
                     RLRHWIFGLPSSQSFRRIEGTDLWHFELELPERSRVEYKIERLIGGHVEWIMDPLNPR
                     VARDPFGANSVCFGEGYETPSWTLADPDARLGTVDEIAVPSRAFGGARRLLLYLPARF
                     RRRRRYPLLVFHDGEDYLRYAALRVVLDNLIHRLEIPPLIAALLVPVARNAEYAAKTT
                     HAEFLRHELVPLLERRLPLIPRTDSRALVGASFGAVAALHAAWTYPGFWGGLLLQSGS
                     FAFSDIGQHRRGPQFDPIVRFVNRFREQPGQPAERLFQSCGTYESLIYENRSIVPLFQ
                     STGMELRYVEARDGHNWENWRDRLREGLSWLFPGPLWMVYE"
     gene            3067..4335
                     /locus_tag="VD788_12870"
     CDS             3067..4335
                     /locus_tag="VD788_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77202.1"
                     /translation="MRRKIGLSLGADICWPICFEEILRRLQLDIEWEGERVGFDVERV
                     TIEPFDLRQPCSYDLVIDRLTHWFHPSREWIKKSVIMNDLYVFNNPWSVQSMEKATTY
                     AAMMQLGLPVPDTWLVPPKTYDRTDDLEPTLTRYARLFDLGQLGDALGYPLFMKPYDG
                     GAWVGVTKIDDAEQLRRAYDESGKRVMHLQRAVLPFDLFVRVIGVGPQTRVVRYDPDA
                     PLHDRYKVDFDFVNADEHRLLQEMTLTINSFFGWDFNSCECLRHDGELIPIDFANPCP
                     DSQVTSLHFHFPWIVTSQLRWAVYCATTRRRMRRNLDWQPYYAVLQRGLPYRERLAAY
                     AAIARERLESERFEEFCDEHLAHLDDVAVEFFASDTARDAVRRKVGAIFPEDEVERFT
                     EHFWGLIQFWRKTEIDRIGRTPHGPSGEGT"
     gene            4332..7553
                     /locus_tag="VD788_12875"
     CDS             4332..7553
                     /locus_tag="VD788_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77203.1"
                     /translation="MSIRRRSFHPTVIALVVLAVGAGRATAQSHEWYAVDYGELVDRR
                     QLSHSSESVGVLLDKLAGRPLPPPDAPGERPDDRYAHSLLEPLLEPYAFVLPDLIDTL
                     AGVPDPPLVELGALWHRGEAQPAWVELLRARRFVVESDGNGRLRLFLPLPAGPPAREA
                     KSAEQAKAAYEGAWSVLRHLFAAERLRLAAGSSELPELRVTAYAYDHQPARTLFLLGA
                     EPYAVTVRDTLASGSRPPLDLAGLERFLDQGLQIEGGRLEGDGTLRVFGSTVEPRPSL
                     LGRPLGLADLAVAYRAVFHGGLAEPYMSLDRGLSPQTALVNYGGRLRDTALGRVSLDC
                     DIRFKTFSLGIDVTTGADLRQELRRAIPGFRTHLERFAADPASAGVQAQQTRLWFYPD
                     AVDLTVSPQIDVLAMRRTRMSAASERLASATSVESREDPDWTRETVADINLRYDEFSR
                     RFPELSDLDQVVRLLSFFTWLKQAELAGLTLPDLDSLLAVELPQRYTPREFPQLLTFD
                     ALPEAGRDDEVVVFDRVAVGDALERLGPRGDAELPARLRFERARAALDAGNSQEAELL
                     TELAGYDLDGTDPDVFDLLAYRAERLRMHRTVLATLGPELGRGLAERTRAGERLRIFS
                     VGIGGLDLGMGRALARASGTSIQLTSVRSPGATGSLAPARSVTLGESRDDWRLASATT
                     PSSAPPDHGFSSPAVDRLRLLTERGVDRTLGGFALAVHGTVDPELQSRRVYFDDDRKA
                     TGIERVDGPRQWRYRFVGDGQRVRATLDPPPAPAEDAPPPAEVPVGLVQLMLTEDPTH
                     VSPSRRVRLRFSQDDVARQLEADFPRELLQRLVLGRGLDLAPGQPMPLAPLPASLGAV
                     EAVMFGLPPGAGRPPWEVRPTVVPGEDDAVRLARALRSWSDDGPVEQAAPPSVVGTDP
                     LRSPQRWRDAPRLAGGGLLLVPDGAFVGRDERLRAELIRRFRSGPVVPTLPAVPTSSL
                     IILVSDEPIGLCSSRLARLAADPQLAGKLLAGWCLAGELRPDVPSSLLARGGLGAVGI
                     ASSAPVDLRHAVATLDTLSSVLGAGELVGRRVETLPGPFVWYY"
     gene            complement(7547..7900)
                     /gene="grxD"
                     /locus_tag="VD788_12880"
     CDS             complement(7547..7900)
                     /gene="grxD"
                     /locus_tag="VD788_12880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HXV77204.1"
                     /translation="MNPTASRSIEEWTEQIKQDVRENRVFVYAKGEKNAAMCGFSARV
                     MQILNQVGTPFEVRNIFADPNIRPAICAYTNWPTIPQVFIDGNFVGGCDILTEMYQSG
                     ELEKMLDSGAAERRQ"
     gene            complement(7920..8153)
                     /locus_tag="VD788_12885"
     CDS             complement(7920..8153)
                     /locus_tag="VD788_12885"
                     /inference="COORDINATES: protein motif:HMM:NF013851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HXV77205.1"
                     /translation="MIVAEEVKRRIEDALPGATVEVRAFSGEDHLQASVVAEQFAGKS
                     RIEQHRMVYASLAGLIGDAVHALQLETRVPGEG"
     gene            8293..10791
                     /locus_tag="VD788_12890"
     CDS             8293..10791
                     /locus_tag="VD788_12890"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HXV77206.1"
                     /translation="MSCRRRLLLALTPWLVAAGASSPAAVDTAPPPTVEAITRGLERR
                     EGLLELFVDRAAGKVWLVLPAPGAHGEVGRFLHVAGLSGGLGSNPVGLDRGLLEKTRL
                     ISIRRLGRRVLVEEVNLRFRAISDEAAERDAVRQSFARSVLWGQEVSASDRSGRAVVD
                     FTSFIVRDAIGVAERLEQSGQGSYRFDPERSALELEQCHAFPQNLEFEAVLTWTGGDP
                     ADHVAEVVPSPDAITLVQHQSLLRLPDDDYRPRRFDPRAGSFAIEFYDYAQPLDRPIE
                     RSWIVRHRLEKTDPSAERSTVKQPIVYYVDRGVPEPVRSALVDGARWWAAAFDAAGLV
                     DAFRVELLPADAHPLDVRYNVIEWVHRSTRGWSYGGGIVDPRTGERIKGHVRLGSLRV
                     RQDRLLFEGLVGTAASGSGAAGDPVELALARIRQLAAHEVGHALGFAHNFAASTYGGR
                     ASVMDYPAPLVRIDARGELDLSEAYTTEIGLWDRFAVAYAYSDFPPGVAADDALDALV
                     ANAIARGWLFLGDEDARPPGAAHPSANLWDNGADPVEELAQAMRVRRIALDRFGVDNV
                     AGGRPLASLEEVLVPVYLHHRYQLDAAAKTIGGLVYAHALRGDGQPPARPVDPAQQRR
                     ALDVILSTLDPVELELPPSITRLLLPRSPGLAPGSERFTGFADPVFDPLAAAATAAEM
                     TITALLQPARATRLVQFHLADPALPSFESLLESLTDRVFASVPHGSESEARREIRRET
                     QRVLSGALIRLARSAEAPASVRVRVDGQLALLRETLERGAGDDAQRRFLRAEIGRHLD
                     RPADAAPLPAAAPAPPPGSPIGAPLGDCSAAADR"
     gene            10853..11395
                     /locus_tag="VD788_12895"
     CDS             10853..11395
                     /locus_tag="VD788_12895"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HXV77207.1"
                     /translation="MSIWRMLGLGQVGGDERGSSAGRDTVRQIVDELADLPIERARYI
                     AMFAFILSRVAGADSKISPAETRAMERIVVEAGGLPEEQAMIVVQMAKTQSLLFGGTD
                     NYSVTKEFAKLASREQKFSLLRCLFAVSAADQSIATVESNVIRQIADELGLDHADFAA
                     LRSEYRQHLAVLRPAKPDEA"
     gene            complement(11402..11602)
                     /locus_tag="VD788_12900"
     CDS             complement(11402..11602)
                     /locus_tag="VD788_12900"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HXV77208.1"
                     /translation="MPLIEIHLLEGRTAEDKKKLLASVTQAVADSIDAPLQSIRVWIN
                     ELSADDYMVAGHMASDRRKPSG"
     gene            11740..12648
                     /locus_tag="VD788_12905"
     CDS             11740..12648
                     /locus_tag="VD788_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77209.1"
                     /translation="MDNPPARDSRRWIVGGSVALLLAVWLVWWQWDGIAGFWSGERAA
                     DGSSAIVAEPAPVNGGEADAAGQTLAEREWIEQLGEAPHWPEDLVEPQSCEDVEAGLA
                     RICAVLDGREYVRAAAPPGGTCGLLRTVAGELAVRPPELTSELKNYPIMLENVFYIYR
                     ALGRERLSLVRRIQFEEQVLAEPAAMAIYRWLASREGCASASRTTMTTEALYGYAGYF
                     FTTMGGQAYLRRRSPRIEALASFYALLFLERAQRADHDPAGVDPRAEIQRTRDLIASQ
                     PLVFRDHYLVLLDRMAESWKRRGESS"
     gene            12690..14423
                     /locus_tag="VD788_12910"
     CDS             12690..14423
                     /locus_tag="VD788_12910"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXV77210.1"
                     /translation="MGQRLRLTASLCLLSILLSASAAAASPPIAAESEHEPSEVSVPA
                     GAVRAFIDAARAGDYRAAAERLDLSRLPAQQPDELGPGLARKLKVVLDRRLWIDYDEL
                     SEREEGAADDGLAADLDRIGTITNGRRWDLLLRRVADEEGRRVWKFAPSTVAAIEPLY
                     DAHGYGWLGDKLPPVMFDWSFLEIRLAQWIGLLLLSLAAYLGARVAVWVLRRLVRPVV
                     ARTRSTLDDRLLDRLATPSRVLVAVGLFTAGSLALRLAEPVQPFVTGIGRGLVLVALA
                     WIALRAIEVGTELLDARFLAWGRPEARAILPLARRVAQGLLIAIAAISALQNLGFNVT
                     GLIAGLGVGGLALALASQKTVENLLGGVTLFVDQPVRIGDFCRFADGKLGTVEEIGMR
                     STRIRTLDRTLVSIPNSRFAELELENYAMRDRIWLSATIGLRYETSGDQLRFVLAGLR
                     KMLVGHPRVCSEPLRVRLAGFGASSLDVELFAYVDTRDFNEFTAIREDILLRVIDVVE
                     QAGTGFAFPSRTVYLGRDSGLDREACERAEASVRRWRETGTLPFPDLPDEEAAAIRDR
                     LDYPPAGSPGS"
     gene            complement(14425..14697)
                     /locus_tag="VD788_12915"
     CDS             complement(14425..14697)
                     /locus_tag="VD788_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77211.1"
                     /translation="MAFMGGSAYNMAREVAEGYVQVTERTFRAMSAGELGQLAHEMDR
                     HQRELRGEPSANDETAQLQARQRKLLRLRTAINVLRAFQQRNRVGA"
BASE COUNT         2043 a         5140 c         5277 g         2448 t
ORIGIN      
        1 cgctcgacct cgcggctgat gcgggcccag ttcagccgcg tcggacatac cggctcctgg
       61 tcgcaaacac ccggctcgac gccgcactgc accatgctga tcgggccctc gatggcgcgg
      121 atgatctcgg cgacggtcag cgtgtcggcc ggccggtcca gactgtagcc gcccgagacc
      181 ccccgacgcg agaccaggat ctccccgcgg gcgagggatc gcatgatctt gctgaccatc
      241 gggaacggga ggccgcagcg ttcggcgacc tcccgtgcgg tctgtacctc gccggacggt
      301 ctctccgcca tcgccacgag cagcatgatc ccgtagtcgg cttccttggt gatccgcagc
      361 atccgtcggc cctctcgcgc cgttttcgaa acccgaccga tcgtatccgg tatcggatag
      421 tgtaccgaat ccgtcggatt tttccagggt cactttttcc tggagaaaag cgcggtcagc
      481 cgggccagat gtcgttgtcg gcgatctcga gtaggttgcc gtccgggtcg tggaaataga
      541 acgagcgtcc gtttcgccag cgcgtttcat ggagcagcgc caccccccgc tcgggcaggt
      601 atcgcttcca gcgatcgtag tcgtcggccg ggacgcggaa gcaggtgtgg accggaccgc
      661 gcgccccgtg tgccggcagc gtgccagcgg ccagcgtttc gtccgcgtcg aacagcagga
      721 agacgctggc gccggcgcgg aagaacaggc tgcgccgcgg ctcggacgcg agcaggcgca
      781 tgcccagaag atcgcggtag aaggcgaggg cacgctgttg gtcggtgtag tacagcacgg
      841 tctcgagcac cgagcccagc gaaggtcgtg cggtggtcat cggtcgcgct cccgtccgga
      901 ccggaatgat acccttggag gtccgccgac cgagcctggc gttcgcaggc ggacaacgaa
      961 cggatgaaga tgcctcgcgc catcttgctc ggtccccagc ggttccgtcc gaacgtggcc
     1021 gctgcgctcg attggctcgg ccgcgacgga ccgctggccc tggtcagcgc gggctggcaa
     1081 gagcgcgagg acgaaatcga cgagttcgtc gagcactgcc gccgcgaggt cgtaccgctc
     1141 ggactgtacc accgcgccga gcgggcgctg gcccgcgacg agctgttgac cgccgcgctg
     1201 cgcgagcgac aggaccaact gcgcgagttg caggggttgt atcgcgtgcg cctggcgtcg
     1261 ctgctcgagg cggcgcgctc actgatgcgc cggggcgacg gacgattcgt cgacgagcac
     1321 cgcaaggcgg cgatccgcgc cgtgcgcacg ctcgatcgcc aacacctcgt ccgcatccgg
     1381 gagatccacg acgagttcga gcgccgggtc cggccggggg cccaccccag cgtccgcgcc
     1441 gagcgggagg agctgcaaca gctcgtcggt cgctgctcgg tgatcgcgat cgccggcggt
     1501 cacgtcgccg tgttgctcaa tcggctgcgt ctgttcgacg tgctcggcct ggcagccgac
     1561 cgcccggtcg tcgcctggtc tgccggggca atggcgctcg gcgagcgcgt gatcctgttc
     1621 cacgactcgc cgccgcaggg gccgggcgac gccgaggtgt tcgagccggg cctcggcctg
     1681 taccgcggcc tggttccgct gccgcacgcc agcgagcgac tcgcgctcga cgacccgctg
     1741 cgggtcgcgc tcttctcgcg ccgcttcgcg ccggcgtcga gcgtcacgct cgagtacggg
     1801 tcgcgcgtcg actggagcgg cgaccgatgg gtcgcgcggg gcgaggtcgg gctgctcggt
     1861 cgaagcggtc gcctgcggcg gatgcgtccg gattgaacgc gatgttcgag cgctggatcg
     1921 ccgacggccc gcccgcggcg gacgagttgt cgcggcggct ggccgagcac gtctttccgc
     1981 tggtcgagcg tcgctcggcg accttcgcct atcacggaaa ggccgacgac gtccggttgc
     2041 gtcactggat cttcgggctg ccgagctcgc agtcgttccg gcggatcgaa ggcaccgacc
     2101 tctggcactt cgagctggaa ctgcccgagc gctcgcgggt cgagtacaag atcgagcggc
     2161 tgatcggcgg acatgtcgag tggatcatgg atccgctgaa ccctcgggtc gcccgcgatc
     2221 cgttcggggc gaactcggtc tgcttcggcg agggctacga gacgccgtcg tggacgctgg
     2281 ccgacccgga cgcgcggctg ggcacggtcg acgagatcgc cgtgcccagc cgtgctttcg
     2341 gcggggcgcg ccggctgttg ctctacctgc ccgcccgctt ccggcggcgc cgacgctacc
     2401 cgctcctcgt ctttcacgat ggcgaggact acctgcgcta tgcggcgctg cgagtcgtgc
     2461 tcgacaacct gatccatcgg ctggagatac cgccgctgat cgcggcgttg ctcgttcccg
     2521 tcgcgcgcaa cgccgagtac gcggccaaga cgacgcacgc ggagttcctg cgacacgagt
     2581 tggtgccgtt gctcgagcgc cgcctgccgt tgattccgcg taccgactcg cgcgcgctgg
     2641 tcggcgccag cttcggcgcc gtggccgcgc tgcacgctgc ctggacctac ccggggttct
     2701 ggggtgggct gctgttgcag tccggttcgt tcgccttctc cgacatcggc cagcatcggc
     2761 gggggccgca gttcgacccg atcgtccggt tcgtcaaccg gttccgcgag caaccgggac
     2821 aacccgcgga acggctgttc cagagttgcg gaacgtacga gtcgttgatc tacgagaacc
     2881 ggtcgatcgt cccgctcttt cagtcgacgg ggatggagct gcgctacgtc gaggcgcggg
     2941 acggacacaa ctgggagaat tggcgcgatc gcctgcgcga gggcctgtcc tggctctttc
     3001 cgggaccgtt gtggatggtg tacgaatgat gcgaccatcg atggacggag gtgcgacgtg
     3061 gcgcgggtga gacggaagat cggcttgtcg ctcggggccg acatctgctg gccgatctgc
     3121 ttcgaggaga tcttgcgtcg gctgcagctc gacatcgaat gggagggtga gcgggtcggg
     3181 ttcgacgtcg agcgcgtgac gatcgagccg ttcgatctgc gtcagccctg ctcgtacgac
     3241 ctggtgatcg accggctgac gcactggttc caccccagcc gggagtggat caagaaatcg
     3301 gtgatcatga acgatctcta cgtcttcaac aacccgtggt ccgtgcaatc gatggaaaag
     3361 gcgacgacct acgccgcgat gatgcagctc gggctgccgg tgccggacac ctggctcgtc
     3421 ccgccgaaga cctacgacag gacggacgat ctcgagccga cgctgacgcg ctacgcacgt
     3481 ttgttcgatc tcggccagtt gggtgacgcg ctgggctatc cgctgttcat gaaaccgtac
     3541 gacgggggcg cttgggtcgg cgtgaccaag atcgacgacg ccgagcagct gcgacgagcc
     3601 tacgacgaga gcggcaagcg ggtgatgcac ctgcagcgcg cggtgctgcc attcgacctg
     3661 ttcgtgcggg tgatcggcgt cggtccgcaa acacgggtcg tacgctacga tcccgacgcc
     3721 ccgctgcacg atcgctacaa ggtcgatttc gacttcgtca atgcggacga gcaccggttg
     3781 ctgcaggaga tgacgttgac gatcaacagc ttcttcggct gggatttcaa ctcgtgtgaa
     3841 tgcctgcggc acgacggcga gctgatcccg atcgacttcg ccaacccgtg tcccgactcc
     3901 caggtcacct cgttgcactt ccactttccc tggatcgtga cctcgcagct gcgctgggca
     3961 gtctactgcg ccacgacgag acgccggatg cggcgcaatc tcgattggca gccgtactac
     4021 gcggtgttgc agcgcggctt gccctatcgc gagcgcctgg cggcctacgc cgcgatcgcg
     4081 cgcgaacggc tggagagcga acgcttcgag gaattctgcg acgagcatct ggcgcacctc
     4141 gacgacgtcg ccgtcgagtt cttcgccagc gacacggcgc gagacgcggt ccggcgcaag
     4201 gtcggggcga tctttcccga ggatgaggtc gagcgattca ccgagcattt ctgggggttg
     4261 atccagttct ggcgcaagac cgagatcgat cgcatcggcc gcacgccgca cggcccgtcg
     4321 ggggagggaa cttgagcatc cgacgacgca gctttcaccc gacggtcatc gcgctggtcg
     4381 tgctcgccgt cggtgcgggt cgcgcgacgg cgcaatccca cgaatggtat gcggtcgatt
     4441 acggcgagct cgtcgatcgc cggcagctga gccattcgag cgagtcggtc ggggtgctgc
     4501 tcgacaagct cgccgggcgt ccgctgccgc cgcccgacgc gcccggcgaa cgacccgacg
     4561 accgctacgc ccattccctg ctcgagccgc tgctcgaacc gtacgccttc gtcctgccgg
     4621 acttgatcga cacgctggcc ggcgtgcccg acccacccct cgtcgagctc ggggcgctgt
     4681 ggcaccgcgg cgaagcccaa ccggcgtggg tcgagctgct gcgcgcccgg cgcttcgtcg
     4741 tcgagagcga cggcaacggg cgactgcggc tgttcctgcc ccttcccgct ggtccgccgg
     4801 cgagggaggc gaagtcggcc gagcaggcga aggcggccta cgagggcgcc tggtccgtgc
     4861 tccgtcacct gttcgccgcc gaacggctgc ggctggctgc cggctcgagc gagctgccgg
     4921 agctgcgggt gacggcctac gcctacgacc accagccggc gcggacgctg ttcctgttgg
     4981 gggcggaacc ctacgcggtg acggtccgcg acacgctcgc cagcggatcg cgcccaccgc
     5041 tcgatctcgc cggcctcgag cgattcctcg accaagggct gcagatcgag ggcgggcgtc
     5101 tcgagggcga cggcacgctg cgcgtcttcg gcagcaccgt cgagccgcgg ccatcgctgc
     5161 tcggtcgccc gctcggactc gccgacctgg cggtcgccta tcgcgccgtg ttccacggcg
     5221 ggctcgctga gccctacatg agcctcgatc gcgggctctc gccgcagacg gcgctcgtca
     5281 actacggcgg gcgcctgcgc gacacggcgc tcggccgggt gagtctcgac tgcgacatcc
     5341 gcttcaagac cttcagcctg ggaatcgacg tgacgacagg tgcggatctg cgccaggagc
     5401 tccgtcgggc gatcccgggg tttcgcacgc acctcgagcg gttcgccgcg gaccccgcct
     5461 cggcgggtgt gcaggcgcag cagacacgtc tgtggttcta ccccgatgcg gtcgacctga
     5521 ccgtctcccc gcagatcgac gtgctggcga tgcggcgcac gcggatgtcg gcggcgtccg
     5581 agcggctcgc ctcggcgacg tcggtcgaat cgcgcgagga ccccgactgg acgcgcgaga
     5641 cggtcgccga catcaacctg cgttacgacg agttttcgcg gcgcttcccc gagctgtccg
     5701 acctcgatca ggtcgtgcgc ttgctctcgt tcttcacctg gttgaagcag gcggagctgg
     5761 ccggcctgac gctacccgac ctcgactcgc tgctcgccgt cgagctgccc cagcgctaca
     5821 cgccacgcga gttcccgcag ctgttgacct tcgacgcgct gcccgaggcc ggtcgcgacg
     5881 acgaggtcgt ggtcttcgac cgcgtcgcgg tcggtgacgc gctcgagcga ctcggtccgc
     5941 gcggtgacgc cgagctgccg gcccgcctgc gtttcgagcg agcccgggcc gcactcgacg
     6001 cgggcaactc ccaggaggcg gagttgctca ccgagctcgc gggctacgat ctcgacggga
     6061 ccgaccccga cgtgttcgac ctgttggcct accgcgccga acggctgcgc atgcaccgca
     6121 cggtgctggc gacgctcggc cccgagctcg gccgcggcct ggccgaacga acgcgggccg
     6181 gtgagcggct gcgcatcttc agcgtcggca tcggcggtct ggatctcggt atgggccggg
     6241 cgctggcccg cgctagcggt acgagcatcc agctcacgtc ggtgcgctcg cccggcgcga
     6301 cggggtcgct cgctcccgcc cgatcggtga cgctcgggga gtcgcgagac gattggcggc
     6361 tggcctccgc cacgacgccg tcgtcggcgc cgcccgatca cgggttctcg tcacccgcgg
     6421 tggatcgcct gcgcttgctg accgaacgcg gtgtcgacag gacgctcggt ggctttgcgc
     6481 tcgcggtcca cggcacggtc gacccggaat tacagagccg ccgcgtctac ttcgacgacg
     6541 accgcaaggc gacggggatc gaacgggtcg atgggccgcg acagtggcgc tatcgcttcg
     6601 tcggagacgg acagcgggtg cgcgccacgc tcgacccgcc tccggcaccg gcggaggacg
     6661 cgccgccgcc ggccgaggtg ccggtggggc tcgtgcagct catgctgacc gaggatccga
     6721 cgcacgtctc gccgagccgg cgcgtccgcc tgcgcttctc gcaggacgac gtggcacggc
     6781 aactcgaggc cgatttcccg cgcgagctgc tccagcggtt ggtgctcggc cgtgggctcg
     6841 acctcgcccc cgggcagccg atgccgctcg cgccgctccc ggcgtcgctc ggcgcggtcg
     6901 aggcggtgat gttcgggctg ccgccgggag caggtcggcc gccgtgggag gtccgcccga
     6961 ccgtcgtgcc gggggaggac gacgcggtcc ggctggcgcg ggccctgcgc agctggtccg
     7021 acgacggacc ggtggagcag gccgcgccgc cgtcggtcgt cgggaccgac ccgctgcgga
     7081 gcccccagcg ctggcgcgac gcgcctcgct tggcgggcgg ggggctgttg ctcgttccgg
     7141 acggcgcgtt cgtcggtcgc gacgagcggt tgcgggcgga gctgatccgc cgcttccgct
     7201 ccgggcccgt cgtgccgacg ctgccggccg tgccgacgtc gtcgctgatc atcctggtca
     7261 gcgacgaacc gatcgggctc tgcagctcgc ggctcgcccg gctggcagcc gatccgcagc
     7321 tggcgggcaa gctgctcgcc ggctggtgcc tcgcgggaga gcttcgaccg gacgtcccct
     7381 cgtcgcttct cgcccgcggt ggcctcggcg cggtcgggat cgcgtcgtcg gcaccggtcg
     7441 atctgcgtca cgcggtcgcg acgctcgaca cgctgtcgtc ggttctcggt gcgggagaac
     7501 tcgtcgggcg gagagtcgag accctccccg gaccgttcgt ctggtattac tgacgccgct
     7561 cagccgcacc cgagtcgagc atcttctcca gctcgccgga ctggtacatt tcggtgagga
     7621 tgtcgcagcc gccgacgaag ttgccgtcga tgaagacctg cgggatcgtc ggccagttgg
     7681 tgtaggcgca gatcgcgggc cggatgttcg gatcggcgaa gatgttccgc acctcgaacg
     7741 gggttccgac ctgattgagg atctgcatca cgcgcgcgga gaatccgcac atcgccgcgt
     7801 tcttttcgcc cttggcgtag acgaagacgc gattctctct cacgtcctgc ttgatctgtt
     7861 cggtccactc ttcgatcgat cgggatgccg tcgggttcat cgtacgctcc tcgcgccggc
     7921 tagccttcgc ccgggacgcg ggtctcgagt tggagtgcat gcaccgcgtc gccgatcagg
     7981 ccggcgagcg aagcgtagac catccggtgc tgctcgatcc tcgacttgcc cgcgaactgc
     8041 tcggcgacga ccgacgcctg cagatggtcc tcaccggaga aggcccgcac ctcgaccgtt
     8101 gctccgggca gtgcatcttc gatccgccgc ttgacttcct cggcgacgat cacgggcgct
     8161 tctccctctt cgacaatccg ggagtatcgc aaacgaccgg cggatttgcg gcaccgcgac
     8221 cttgacggcg gcggtgcgag cggtcaacct tgccgttcgt tcgcgcctgc acgcctgtac
     8281 ggaggaccat cgatgagctg ccgccgccgc ctgctgctcg cgctcacccc gtggctcgtc
     8341 gcggccggtg cgtcgtcgcc ggcggccgtc gacaccgcgc cgccaccgac ggtcgaggcg
     8401 attactcgtg gactcgaacg gcgcgagggg ctgctcgagc tgttcgtcga tcgtgcggcg
     8461 gggaaggtct ggctggtgct gcccgcgccg ggcgcgcacg gcgaggtcgg gcgtttcctg
     8521 cacgtcgccg ggctgagcgg cgggctcggc tcgaacccgg tcggtctcga tcgaggtttg
     8581 ttggagaaga cgcgattgat ctcgatccgc cgcctcggtc gacgcgtgct ggtcgaggaa
     8641 gtcaacctgc gctttcgcgc gatctcggac gaagccgccg agcgcgacgc cgtccgccag
     8701 tcgttcgccc gttcggtgct gtggggtcag gaggtctccg cgagcgaccg ctcgggacgc
     8761 gcggtcgtcg atttcacgtc gtttatcgta cgcgatgcga tcggggtcgc cgaacgtttg
     8821 gagcagagcg ggcagggcag ttaccgcttc gatcccgagc gcagcgcgct cgaactggag
     8881 cagtgccatg cgttcccgca gaacctcgag ttcgaggcgg tgttgacctg gaccggcggc
     8941 gatccggcgg accatgtcgc cgaggtcgtc ccgtcgcccg acgcgatcac gctggtccag
     9001 caccagtcgt tgctgcgact gccggacgac gactatcgac cccgccgctt cgatccacgg
     9061 gccggctcgt tcgcgatcga gttctacgat tacgcccagc cgctcgatcg cccgatcgaa
     9121 cgatcgtgga tcgtgcgtca ccggttggag aagaccgatc cgtccgcgga gcggtcgacg
     9181 gtcaagcagc cgatcgtcta ctacgtcgat cgcggagttc ccgagccggt ccgctcggcg
     9241 ctggtcgatg gagcccgctg gtgggccgcg gcattcgacg cggccgggtt ggtcgacgcg
     9301 ttccgcgtcg agcttctgcc cgcggacgcg cacccgctcg acgtgcgcta caacgtgatc
     9361 gaatgggtcc accgctcgac gcgcggctgg tcctacggcg gcgggatcgt cgacccgcgc
     9421 accggcgagc ggatcaaggg acacgtccgg ctcggctcgt tgcgggtgag gcaggaccgt
     9481 ctgctgttcg agggcctcgt cgggaccgcg gccagcggat cgggtgcagc gggcgatccc
     9541 gtcgagctgg cgctcgcccg catccggcag ctcgcggccc acgaggtggg tcacgcgctc
     9601 gggttcgccc acaacttcgc cgccagcacc tacggtggga gagcctcggt gatggactac
     9661 ccggcaccgc tggtacgcat cgacgcgcga ggcgagctcg acttgagcga ggcctacacg
     9721 acggagatcg gtctgtggga tcggttcgcc gtggcctacg cctactccga cttccccccc
     9781 ggcgtcgccg ccgacgacgc gctcgacgcg ctcgtcgcga acgccatcgc acgcggctgg
     9841 ctgttcctcg gcgacgagga cgcacgtccg ccgggcgcgg cccacccatc ggccaatctg
     9901 tgggacaacg gcgccgaccc cgtcgaggag ctggcccaag cgatgcgcgt ccgccgcatt
     9961 gccctcgatc ggttcggcgt cgacaacgtc gccggcggca ggccgctagc ctcgctcgag
    10021 gaggtgctcg ttccggtcta cctgcatcac cggtaccagc tcgacgccgc ggcgaagacg
    10081 atcggcggcc tggtctacgc ccacgcattg cgcggggacg gccagccgcc ggcgcggccg
    10141 gtcgacccgg cacagcagcg ccgggcgctc gacgtgatcc tctcgacgct cgacccggta
    10201 gagctcgagc taccgccgtc gatcacccgc ctgctcctgc cgcgctcccc gggtctcgca
    10261 cccggcagcg agcgattcac cggcttcgcc gaccccgtct tcgacccgct cgccgcggcc
    10321 gcgaccgccg ccgagatgac gatcacggcc ctgctccagc ccgcgcgcgc tacccgactc
    10381 gtccagttcc acctcgccga cccggcgctg ccgtcgttcg agtcgctgct cgaatcgctg
    10441 accgatcggg tcttcgcgag cgtcccccac ggcagcgagt cagaggcgcg gcgcgagatc
    10501 cgacgcgaga cgcagcgggt gttgagcggg gcgttgatcc ggctcgctcg gtcggccgag
    10561 gcgccggcgt cggtgcgggt ccgcgtcgac ggccagctgg ccctgctgcg cgagacgctc
    10621 gagcgcggcg caggcgacga cgcgcagcga cggttcctgc gggccgagat cggccggcac
    10681 ctcgatcggc cggccgacgc cgcgccgcta cccgccgccg cgccggcccc gcctccgggc
    10741 agtccgatcg gtgcgccgct cggcgactgc tccgcggccg cggaccgctg acgctcggcc
    10801 cgcggggtat catgtcgcgc gggtccgccg accgacccgc cggagggagc cgatgtcgat
    10861 ttggagaatg ctcgggctcg gacaggtcgg aggcgacgag cgcggctcgt ccgccgggcg
    10921 ggataccgtc cggcagatcg tcgacgagct cgccgacctt ccgatcgagc gcgcgcgcta
    10981 catcgcgatg tttgccttca tcctcagccg cgtcgccggc gccgattcga agatcagccc
    11041 ggccgaaacg cgtgcgatgg agcggatcgt cgtcgaggcg ggggggttac ccgaggagca
    11101 ggcgatgatc gtcgtccaga tggcgaagac acagagcctg ttgttcggcg ggaccgacaa
    11161 ctactcggtg acgaaggagt ttgccaagct ggcgagccgc gaacagaagt tctcgctgtt
    11221 acgctgcttg ttcgccgtgt cggcggcgga ccagtcgatc gcgacggtcg agagcaacgt
    11281 catccgccag atcgccgacg agctcgggct ggaccacgcc gatttcgccg cgctgcgatc
    11341 ggagtatcgt cagcacctgg cggtgttgcg accggcgaaa ccggacgagg cgtgacgccg
    11401 ctcagccgct gggcttccgc cggtctgacg ccatgtgacc ggcgaccatg taatcgtcgg
    11461 ccgacagctc gttgatccaa acgcggatcg actgaagcgg agcgtcgatc gagtcggcga
    11521 cggcctgcgt gaccgaggcc agcagcttct ttttgtcctc ggcggtgcgt ccttcgagca
    11581 ggtggatttc gatcagtggc atcggcgcgt gctcctgatc gacccggccg gggcgcgggt
    11641 cgattcacgt ctaccgcaac agggtacacc aaagcccgtg cgcgatcggc tggcgactat
    11701 actgttcgcg tcgccgtcgg cggcggctcg gaggatgagg tggacaaccc ccccgcgcgc
    11761 gactcgcgcc gttggatcgt cggtgggtcg gtcgccctgc tgctcgccgt ttggttggtg
    11821 tggtggcaat gggacggtat cgcgggcttt tggtccggcg agcgagcggc cgacgggtcg
    11881 agcgcgatcg tggcggaacc cgcgccggtg aacggcggcg aggcggacgc ggcgggccag
    11941 acgctcgccg agcgcgagtg gatcgaacag ctcggcgagg cgccgcactg gcccgaggac
    12001 ctcgtcgagc cgcaaagctg cgaggacgtc gaagcggggt tggcgcgcat ctgtgccgtg
    12061 ctcgacggac gcgagtacgt gcgggccgct gcgcctcccg gcgggacctg cgggctgttg
    12121 cgaacggtcg ccggcgagct ggcggtccgt ccgcccgagc tgacgtccga gctgaaaaac
    12181 tacccgatca tgctggagaa cgtcttctac atctatcgcg cgctcggacg cgagcgcctg
    12241 tcgctcgtgc gccgcatcca gttcgaggaa caggtactgg ccgagccggc ggcgatggcg
    12301 atctatcgct ggctggcgag ccgcgagggt tgcgccagcg ccagccgaac gacgatgacg
    12361 accgaggcgc tctacggcta cgccggttac ttcttcacga cgatgggcgg acaggcctac
    12421 ctgcgccgcc gctcgccacg gatcgaggcg ctggcctcgt tctatgcgct gttgttcctc
    12481 gagcgcgcgc agcgcgccga tcacgacccg gccggtgtcg accctcgcgc ggagatccag
    12541 cgcacgcggg acctcatcgc gagccagccg ctcgtcttcc gcgatcatta tctcgtgctg
    12601 ctcgaccgga tggcggaaag ctggaagcgg cggggggaat cgtcatgagc gcgccgccgg
    12661 cgctccacgg aacgagggag gtcggatcga tgggccaacg acttcgcctg accgcgagcc
    12721 tgtgtctcct ctcgattctc ctgtcggcat cggccgcggc cgcctcgcca ccgatcgcgg
    12781 ccgagagcga gcacgagccg tccgaggtga gcgttccggc cggcgccgtg cgcgcgttca
    12841 tcgacgcggc gcgggccggc gactaccggg ccgcggccga gcggctcgat ctgtcgcgcc
    12901 tgcccgcgca gcagcctgac gagctcggac cgggtcttgc acgaaagctg aaggtggtcc
    12961 tcgaccgcag gctgtggatc gactacgacg agttgagcga gcgcgaggaa ggcgcggcgg
    13021 acgacgggct ggcggcggac ctcgaccgga tcgggacgat caccaacggg cggcgatggg
    13081 acctgctgct ccgtcgggtc gcggacgagg agggtcgccg ggtctggaag ttcgcaccga
    13141 gcacggtggc cgcgatcgaa ccgctgtacg acgcccacgg ctacggctgg ctcggggaca
    13201 agctgccacc ggtgatgttc gattggagct tcctcgagat acgcttggcg cagtggatcg
    13261 ggctcttgct gctctcgctc gcggcctatc tcggcgcccg cgtcgccgtg tgggtgctgc
    13321 gccggctggt ccggccggtc gtcgcccgca cccgcagcac gctcgacgac cggctgctcg
    13381 accggctcgc gaccccgagc cgcgtgctcg tcgcggtcgg gctgttcacc gcgggcagcc
    13441 ttgcgctgcg tctcgccgaa ccggtccagc cgttcgtcac cggtatcggg cgcgggctgg
    13501 tgctggtcgc gctggcgtgg atcgctctcc gcgcgatcga ggtcggcacg gagctgctcg
    13561 acgcgcgatt cctcgcctgg ggtcggccgg aggctcgggc gatcctcccg ctggcccgcc
    13621 gggtggccca gggcctgctg atcgcgatcg cggcgatcag cgcgctgcag aacctgggat
    13681 tcaacgtcac cggcctgatc gccggcctgg gagtcggcgg actggcgttg gcgctcgcct
    13741 cgcagaagac cgtcgagaac ctgctgggcg gtgtcacgct gttcgtcgat cagccggtgc
    13801 ggatcggcga tttctgccgg ttcgccgacg gcaagctggg tacggtcgag gagatcggga
    13861 tgcgctcgac gcgcatccgc accctcgacc ggacgctggt gtcgattccg aattcccgat
    13921 tcgccgagtt ggagctcgag aactacgcga tgcgagaccg gatctggctg tcggcgacga
    13981 tcggtctgcg ttacgagacg agcggggacc agctccgctt cgtgctggcc ggcctgcgca
    14041 agatgctggt cggtcacccg cgtgtttgct ccgaaccgct gcgggtacgt ctggcgggat
    14101 tcggcgcctc gtcgctcgac gtcgagctgt tcgcctacgt cgacacgagg gacttcaacg
    14161 agttcaccgc gatccgcgag gacatcctgc tgcgggtgat cgacgtggtc gagcaggccg
    14221 ggaccggatt cgccttcccg tcccggacgg tctacctcgg ccgcgacagc gggctcgacc
    14281 gcgaggcctg cgagcgggcc gaggcgagcg tgcgccgctg gcgggagacg gggacactgc
    14341 cgtttcccga tctgccggac gaggaggccg ccgccatccg agaccgcctg gactacccgc
    14401 ccgccggatc gccgggttcg taggctaggc gccgacgcgg ttccgctgtt ggaaggcccg
    14461 cagcacgttg atcgccgttc gcaagcgcag cagcttgcgc tggcgggcct ggagctgggc
    14521 ggtctcgtcg ttggccgatg gctcgccccg cagctcgcgc tgatgccggt ccatttcgtg
    14581 cgccagctgc cccagctcgc cggcggacat cgcgcggaag gtgcgctcgg tcacctggac
    14641 gtacccctcc gccacctcgc gagccatgtt gtaggcgctc ccgcccatga atgccatcgc
    14701 gtcactccct acccgccgtc gcggcggatc gacgggacaa tcgtagttcc ccgggccggc
    14761 cgaagcacgc gccgacgcaa aacccgccgg ctgcgatgat ccccgtcggt tcgctcgagc
    14821 cgtggtgggg cctgctcgcg ctcgcggtgg tcgcgctctt cgcgggcctc gtgcgcgggt
    14881 tcagcggctt cggcgccggc atggtgat
//
LOCUS       DASPGN010000442         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_197713, whole genome shotgun sequence.
ACCESSION   DASPGN010000442 DASPGN010000000
VERSION     DASPGN010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197713"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="VD788_12920"
     CDS             complement(<1..1535)
                     /locus_tag="VD788_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV77212.1"
                     /translation="MAAANAPVPTGPQPAGRATPHREVDAVLARLEQRKHDWTRVPAD
                     RRAVLLRTCMHGLLDVADEWVRVATEAKGEPPASPVAGEEWISGPMVTLRYLRLLADA
                     LDRDGQPAAAEIRPGPAGRTVARVFPYDLFDRLLYAGLRAEVWIAPDRLPTQGRIYAD
                     KRAGRPGPGRVGLVLGAGNVGSIVPTDALYKMFVEDQVVLVKMNPINAYLGPLLRRAF
                     RCLVDGGFLEFVYGDAEIGGYLCRHERVEAVHLTGSDATYDTIVWGATARERAARKAA
                     GTPLVDKPFTAELGCVSPVIVAPGRWSANEIDFQARHVAAMVAHNASFNCNAAKVLVL
                     PARWTQRGDFLDAVARALALTPPRRAYYPGAERRYRAFLDRYPRAVAVGARGEGVVPW
                     TLVRDVEPTDDEYAFETEAFCGVLAEVTLDAGRTGDPATFLDGAARFVNRALRGSLSC
                     TVLVSDAAREVMGASLERSIAALQYGGIGVNVWAGVLFGLGVTSWGANPGHTRADIGS
                     GVG"
     gene            1665..>2204
                     /gene="selD"
                     /locus_tag="VD788_12925"
     CDS             1665..>2204
                     /gene="selD"
                     /locus_tag="VD788_12925"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1716..1718,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HXV77213.1"
                     /translation="MSGRDAVRLTKLASCAGUASKMGPEALAQVLRPLAGMFDAKLYP
                     DLLQGLDSPDDAAVWRLDAERALVLTADFFPPVVDDPYDFGAIAAANSLSDIYAMGAD
                     PVLGINLVGFPDGLDTSILSEILRGGAEKMREAGAPIAGGHTTSDAEPKYGIATVGFA
                     HPDRLLLKGGARPGDVLFLT"
BASE COUNT          312 a          782 c          797 g          313 t
ORIGIN      
        1 atgccgacgc cggagccgat gtcggcgcgc gtgtgaccgg ggttggcgcc ccagctcgtc
       61 acgcccagac cgaacagcac gccggcccag acgttgaccc cgatgccgcc gtactgcagt
      121 gccgcgatcg agcgctcgag cgacgcgccc atcacttcgc gcgcggcgtc gctgacgagc
      181 accgtgcagg acaggctgcc gcgcagcgcg cggttgacga atcgcgccgc gccgtcgagg
      241 aacgtggccg ggtccccggt cctgcccgcg tcgagcgtga cctcggcgag gacgccgcag
      301 aacgcctcgg tctcgaacgc gtactcgtcg tcggtcggct cgacgtcacg cacgagcgtc
      361 cagggcacca caccctcgcc ccgcgcgccg acggcgacgg cccgcggata gcggtcgagg
      421 aacgcgcgat agcgtcgctc cgcgcccggg tagtaggcgc gccgcggcgg cgtcagggcc
      481 agcgcgcgcg cgacggcatc caggaagtcg ccacgctgcg tccagcgcgc cggcaggacg
      541 agcaccttcg ccgcgttgca gttgaagctg gcgttgtgcg cgaccatcgc ggccacgtga
      601 cgcgcctgga aatcgatctc gttcgcgctc caccgccccg gcgcgacgat gaccggcgag
      661 acgcagccca gctcggcggt gaacggcttg tcgaccagcg gtgtcccggc ggccttgcgt
      721 gcggcgcgct cccgcgcggt cgcgccccag acgatcgtgt cgtaggtcgc atcggagccg
      781 gtcaggtgga ccgcctcgac gcgctcgtgc cggcagaggt agccgccgat ctcggcgtcg
      841 ccgtagacga actcgaggaa gccgccgtcg accagacagc ggaaggcgcg ccgcagtaac
      901 ggtccgaggt aggcgttgat cgggttcatc ttgaccagca cgacctggtc ttcgacgaac
      961 atcttgtaca gtgcgtcggt cgggacgatc gagccgacgt tgccggcgcc cagcaccagc
     1021 ccgacacgac ccggccccgg ccggccggcg cgcttgtcgg cgtagatccg tccctgagtg
     1081 ggcagccggt cgggggcgat ccacacctcg gcgcgcaggc ccgcgtagag caggcggtcg
     1141 aacaggtcgt agggaaagac ccgggcgacc gtacggcctg cggggccggg tcggatctcg
     1201 gcggccgcgg gttgcccgtc gcgatcgagg gcgtcggcca gcaggcgaag atagcgcagc
     1261 gtgaccatcg gtccgctgat ccactcctcg ccggcgaccg gcgacgcggg cggctcgccc
     1321 ttggcctcgg tcgcgacgcg gacccactcg tcggcgacgt cgagcagccc gtgcatgcaa
     1381 gtacgcagca gcacggcccg gcgatcggcc ggcacgcgcg tccagtcgtg cttgcgctgc
     1441 tcgagccggg ccagcacggc gtcgacttcc cgatgggggg tcgctcggcc cgccggttgc
     1501 gggccggttg gaaccggggc gttcgcggca gccacctcgt tcatctccgc tcggttcccg
     1561 ttctcgaccg acggcttcgg tcgcacggtc aaggtaatcc tatcgcgggt tgtcggccac
     1621 cggcgggatg gcgtagcctg gtcagtcaca ggaggcgaat gccgatgtcc gggcgagacg
     1681 cggtccgtct gaccaagctg gcatcctgcg cgggctgagc gagcaagatg ggccccgagg
     1741 ccctggcgca ggtgctgcgt ccgctcgccg gcatgttcga tgcaaagctc tatccggacc
     1801 tgctccaggg gctcgacagt ccggacgacg cggccgtgtg gcgtctcgat gccgagcggg
     1861 cgctggtgct gacggctgat ttcttcccgc cggtcgtcga cgacccctac gacttcggcg
     1921 cgatcgccgc ggcgaactcg ctctcggaca tctacgccat gggggcggat cccgtgctgg
     1981 ggatcaacct ggtcggcttc ccggacgggc tcgacacctc gatcctctcc gagatcctgc
     2041 gcggcggcgc ggagaagatg cgggaagccg gcgcgccgat cgccggggga catacgacga
     2101 gtgacgccga gcccaagtac ggcatcgcca ccgtcggttt cgcgcacccg gatcggctgc
     2161 tgctcaaggg gggcgctcgg ccgggcgacg tgctgttcct gacc
//
LOCUS       DASPGN010000443         5897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1134774, whole genome shotgun sequence.
ACCESSION   DASPGN010000443 DASPGN010000000
VERSION     DASPGN010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5897
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134774"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..919)
                     /locus_tag="VD788_12930"
     CDS             complement(<1..919)
                     /locus_tag="VD788_12930"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXV77214.1"
                     /translation="MPKFTLDGTEIEVAPGTTILQAALARGQEIPHFCYHPGLSIAGN
                     CRICLVEVEKAPKLAIGCMTQALDGMVVHTDSDRVREAQNAVMEFLLINHPLDCPICD
                     QAGECKLQDQAVEYGPGRSRYTEPKLALAKAVDIGRHVMLDQERCIQCSRCTRFCDEV
                     TGTGELAFFQRGDRSQIGIYPGKRLDNPYSGNVVDICPVGALTLKEFRFATRVWYLKN
                     TPSICVGCARGCNVMIATGRQRELMTTIGQQDERIKRLVPRVNIEVNGHWICDEGRLS
                     YRRLEAAPLLAQAAAPAGTRTEYHAAVARA"
     gene            complement(912..2189)
                     /gene="nuoF"
                     /locus_tag="VD788_12935"
     CDS             complement(912..2189)
                     /gene="nuoF"
                     /locus_tag="VD788_12935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXV77215.1"
                     /translation="MERVLTRNIHKADSQSIEVYEADGGYRAYRKAVTSMAPDEVTDQ
                     VKRSGLRGRGGAGFPTGLKWSFMPKQKTKPHYLLLNADESEPGTFKDRLLMEKDPHLV
                     IEGSMISAYAIRADTCYVYIRGEFVHGARVMQQAVDEAYAKGYAGQNVFGSDWSCELV
                     VHRGAGAYICGEETALMTSLEGDRGWPRNKPPFPAQAGFFGNPTTINNVETLACVPFI
                     IERGVDWFLSLGPNEKNTGPKLYCLSGHVKRPGVYEAPMGLPLNELIYDLGGGMLHPD
                     RPLKACIPGGSSVPVITADECDVDLDFDSLAKIGSALGSAGIMVMDSSTCMVKALLRI
                     AYFYAHESCGQCTPCRQGTGWLRRILERIEAGQGRPADVDLLKSVADNIEGNTICALG
                     DAAAWPVQSFVKKFRDEFVAHVEAGKCTVLSNA"
     gene            complement(2199..2663)
                     /locus_tag="VD788_12940"
     CDS             complement(2199..2663)
                     /locus_tag="VD788_12940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXV77216.1"
                     /translation="MFGPEFEKRVDALIGNYPEPRAALLPILWEIQRERGWIDGDCEE
                     WAGKRVGVSAAHVHGVVTFYTMYKQRPSGKYHIQVCTTLSCMLRGSDELLDHLERKLG
                     IGVGEVTADGRFSLQRVECLGSCGTAPMFQLNDDYHEDLTPERVDRLLDGLP"
     gene            complement(2670..3872)
                     /gene="nuoD"
                     /locus_tag="VD788_12945"
     CDS             complement(2670..3872)
                     /gene="nuoD"
                     /locus_tag="VD788_12945"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HXV77217.1"
                     /translation="MSTDHVFERHPDHHDIVTVNMGPSHPATHGVLRLVLRLDGERVV
                     DCVPHLGYLHRGMEKIAENRTYLQFIPYTDRMDYLSPLAANVGFALAVEELLGIEVPE
                     RCKAIRVICCELARIGSHQLWLGTHALDLGAATVFFHTFRDREWHYDLVEDLTGARLT
                     TSFTRVGGVMHDVTADWLARLKEFATAVPGRVDEYESLLTNNAIWLKRTQGVGALTAE
                     QAISFGMTGPSLRATGVDHDLRKKRPYSGYEKYEFDVPVGSEGDIYDRYLVRLEEMRQ
                     SARILLQAIDNLPDGPLNIDDPKIFLPPKQGVLTKMEELIHQFMIITEGFACPSGEIY
                     HSTEVPKGELGFYIVSTGGRAPYRLRIRSPSFNNLATMPLLVEGGLVADVIANIASLD
                     PVMGEVDR"
     gene            complement(3897..4370)
                     /locus_tag="VD788_12950"
     CDS             complement(3897..4370)
                     /locus_tag="VD788_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXV77218.1"
                     /translation="MGVNRVADGQAAVERIKQRFADAVEDSGSYRGQHWVEVAGDRLV
                     EVCEWLRDDPANAYDYLVDVTAVHLPDDPRPMQMVYHLYSHSHDDRLRVKTRADDELV
                     VPTLSDLWQSANWNERETYDMFGIRFDGHPDPRRILMPDDYTDFPLRKEFPLFRG"
     gene            complement(4361..4729)
                     /locus_tag="VD788_12955"
     CDS             complement(4361..4729)
                     /locus_tag="VD788_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017532373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXV77219.1"
                     /translation="MSDSRLTVLLVLLIAGGMAGALLALSHLIGRVRGSVVDQTPYEC
                     GMKPYEYAERRRFSIHFYLVAMVFILFDIEVAFLFPWAVTFRTFDADRLFVLGEMFVF
                     LGILIVGFVYLWKRGALEWE"
     gene            complement(4956..>5897)
                     /locus_tag="VD788_12960"
     CDS             complement(4956..>5897)
                     /locus_tag="VD788_12960"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77220.1"
                     /translation="GDKFEYHNGLATVHFTKKDYAKAIAALKTAEPLAADNNQKFALY
                     STRGGCYAAMGKWAEAVEDLEQARKINAKKPSVLVTLGQSYYKLGHYDKAVPVFREAV
                     KLVPNDTASVQLLAEALINLGAESTSDSQKDSMYEEAMGYAEKFQSVEPANYEAANLV
                     GRAALGASEYTKAEQAFRKVLSQKPDYCYAMVNLGKTYIAEERWADAEKVLNDAAACA
                     PRMAVIYESLGFSLQKQNRLTEAVTAYEKSYDIKPSESVRNAIEICKQNIAVRDHNAQ
                     IASEEEAQRAAEARAQAEYEEEMKKRKEWEERRKKGD"
BASE COUNT          966 a         1963 c         1880 g         1088 t
ORIGIN      
        1 ccgcccgcgc caccgcggca tggtactcgg tccgcgtgcc ggccggcgcg gccgcctgcg
       61 ccagcagcgg cgctgcttcg agccgtcggt aggacaaccg cccctcgtcg cagatccagt
      121 ggccgttgac ctcgatgttg acacgcggca ccagccgctt gatgcgctcg tcctgttggc
      181 cgatcgtcgt catcagctcg cgctgccgcc cggtggcgat catcacgttg cagccgcgcg
      241 cgcaaccgac gcagatcgac ggcgtgttct tcaggtacca cacccgcgtc gcgaagcgga
      301 actccttcaa cgtcagggcc ccgaccggac aaatgtcgac gacgttgccc gagtaggggt
      361 tgtccagccg cttgccggga tagatgccga tctgcgatcg gtcgcctcgc tggaagaacg
      421 ccagctcgcc cgtacccgtc acctcgtcgc agaaccgggt gcaccggctg cactggatgc
      481 agcgctcctg gtcgagcatc acgtggcggc cgatgtcgac ggccttggcc aacgcgagct
      541 tgggctcggt gtagcggctt cggcccggcc cgtactcgac cgcctggtcc tgcagcttgc
      601 actcgccggc ctggtcgcag atcggacagt cgagcgggtg gttgatcagc aggaactcca
      661 tcaccgcgtt ctgtgcttcg cgcactcggt ccgagtcggt atgcaccacc atcccgtcca
      721 gcgcctgggt catacatccg atggcgagct tcggcgcctt ttcgacctcg accaggcaga
      781 tgcggcagtt gccggcgatc gacagaccgg ggtggtagca gaagtgcggg atctcctggc
      841 cgcgcgccag cgccgcttgc aggatcgtcg tgccgggggc gacttcgatc tccgtcccgt
      901 cgagcgtgaa cttaggcatt ggacagcacc gtgcacttcc cggcttccac gtgagccacg
      961 aactcgtcgc ggaacttctt gacgaacgac tggaccggcc acgccgcggc gtcgccgagc
     1021 gcgcagatcg tgttgccctc gatgttgtcg gccacgctct tcaacaggtc gacgtcggcc
     1081 gggcgaccct gaccggcctc gatgcgctcg aggatgcgac gcaaccagcc ggtgccctgc
     1141 cggcacggcg tgcactgccc gcacgattcg tgggcgtaga agtaggcgat gcgaagcagc
     1201 gccttgacca tgcaggtcga gctgtccatc accatgatgc cggccgagcc gagcgccgaa
     1261 ccgatcttgg ccagcgaatc gaaatcgaga tcgacgtcgc actcgtcggc ggtaatcacc
     1321 ggaaccgacg atccccccgg aatgcaggcc ttcagcggcc gatccgggtg cagcatcccg
     1381 ccgcccagat cgtagatcag ctcgttgagc ggcagcccca tcggcgcctc gtagaccccg
     1441 ggacgtttga cgtgaccgct caggcagtac agtttgggcc cggtgttctt ctcgttcggg
     1501 ccgagcgaca ggaaccaatc gacgccacgc tcgatgatga acggcacgca tgccaaggtc
     1561 tcgacgttgt tgatcgtcgt cgggttgccg aagaagccgg cctgcgccgg aaacggcggc
     1621 ttgttccgcg gccagccgcg atcgccttcg agcgaggtca tcaacgcggt ctcctcgccg
     1681 cagatgtagg cccccgcacc acgatggacg accagctcgc agctccagtc gctgccgaag
     1741 acgttctggc cggcgtagcc cttggcgtac gcctcgtcga cggcctgctg catcacccgc
     1801 gcaccgtgga cgaactcgcc gcggatgtag acgtagcacg tgtcggcgcg gatggcgtag
     1861 gccgagatca tgctgccttc gatgaccaga tgcgggtctt tctccatcag cagccggtcc
     1921 ttgaacgtgc ccggctcgga ctcgtcggcg ttgagcagca ggtagtgcgg cttggtcttc
     1981 tgcttcggca tgaacgacca cttgagaccg gtcggaaacc ccgcgccgcc gcggccgcgc
     2041 agaccggagc gcttgacctg atcggtgacc tcgtcgggag ccatcgaggt caccgccttg
     2101 cggtaggcgc gatagccgcc gtcggcctcg taaacctcga tcgactgcga gtcggccttg
     2161 tggatgtttc tggtgagtac ccgttccatc cctgcctctt acggaagacc gtcgagcagt
     2221 cgatcgacgc gttccggcgt caggtcttcg tggtagtcgt cattcagctg gaacatcggc
     2281 gcggtcccgc acgaacccag gcattcgacg cgttgcagcg agaacctgcc gtcggcggtc
     2341 acctcgccga cgccgattcc cagcttgcgc tcgagatgat cgagcagttc gtccgacccg
     2401 cgcagcatgc aggacagcgt cgtacagacc tggatgtggt acttgcccga cggccgttgc
     2461 ttgtacatcg tgtagaacgt gacgacgccg tggacgtggg ccgcgctgac gccgacgcgc
     2521 ttgccggccc actcttcgca gtcgccgtcg atccagccgc gctcgcgttg gatctcccac
     2581 aggatcggca acagcgccgc gcgtggctcg gggtagttgc cgatcagcgc atcgacccgt
     2641 ttctcgaatt ccggtccgaa catcggtcgc tacctgtcca cttcgcccat caccggatcg
     2701 aggctggcga tgttggcgat cacgtctgcc accagtccgc cctcgaccag cagcggcatg
     2761 gtggcgaggt tgttgaagct gggcgagcgg atgcgcaggc gatacggcgc gcggccgccg
     2821 gtcgagacga tgtagaaccc caactcgccc ttgggtacct cggtcgaatg gtagatctcg
     2881 cccgacgggc aggcgaagcc ctcggtgatg atcatgaact gatggatcag ctcttccatc
     2941 ttggtcagca cgccctgctt cggcgggagg aagattttcg ggtcgtcgat gttgagcggg
     3001 ccgtccggca ggttgtcgat cgcctggagc aggatgcggg ccgactggcg catctcctcg
     3061 agccggacga ggtagcggtc gtagatgtca ccctcgctgc cgaccggtac gtcgaactcg
     3121 tacttctcgt agccgctgta cggtcgcttc ttgcgcaagt catggtcgac gccggttgcg
     3181 cgcaacgacg ggccggtcat cccgaacgag atcgcctgct cggcggtcag cgcgccgacg
     3241 ccctgggtcc gcttgagcca gatcgcgttg ttggtcaaca gcgattcgta ttcgtcgacg
     3301 cggcccggga ccgccgtggc gaactctttg agccgcgcga gccaatcggc ggtgacgtcg
     3361 tgcatcacgc cgccgacgcg ggtgaacgag gtggtcaagc gcgccccggt caggtcctcg
     3421 acgaggtcgt agtgccactc gcggtcgcga aaggtgtgga agaagaccgt tgccgcgccc
     3481 agatcgagcg cgtgcgtccc gagccacagt tggtgcgagc cgatccgggc cagctcacag
     3541 cagatcacgc gaatcgcctt gcagcgttcc ggcacctcga tgccgagcag ctcctcgacg
     3601 gccagcgcga aaccgacgtt ggccgccagc ggactgaggt agtccatccg gtcggtgtac
     3661 gggatgaact gcaggtaggt gcgattctcg gcgatcttct ccatgccgcg gtgcaggtag
     3721 ccgaggtgcg ggacgcagtc gacgacgcgc tcgccgtcga gccgcagcac caggcgcaac
     3781 acgccgtgcg tcgccgggtg cgacggaccc atgttgaccg tcacgatgtc gtgatgatcc
     3841 gggtggcgct cgaaaacgtg gtcggtactc atctcgatcc ctacacggac cggccgctag
     3901 ccgcggaaca gcgggaactc cttgcgcagc gggaaatcgg tatagtcgtc cggcatcagg
     3961 atgcggcgcg gatccgggtg gccgtcgaag cggattccga acatgtcgta ggtctcccgc
     4021 tcgttccagt tggccgactg ccacaggtcg gacagcgtcg gtacgacgag ttcgtcgtcc
     4081 gctcgagtct tgacacgcag acggtcgtcg tgggaatggc tgtaaaggtg gtaaaccatt
     4141 tgcatcggcc gcggatcgtc cggcaagtgc accgccgtca cgtcgaccaa atagtcgtag
     4201 gcgttcgccg gatcgtcgcg cagccactcg caaacctcga ccagacggtc gcccgccacc
     4261 tcgacccagt gctggccgcg gtacgacccg ctgtcctcga ccgcgtcggc gaaccgctgt
     4321 ttgatccgct cgaccgctgc ttgaccgtcg gccacccggt tcactcccat tccagtgcgc
     4381 cgcgtttcca caggtagacg aatccgacga tcaggatccc gagaaaaacg aacatctccc
     4441 ccagcacgaa cagcctgtcg gcgtcgaagg tccggaaggt gacggcccag ggaaacagga
     4501 atgcgacctc gatgtcgaac aggatgaaga ccatcgccac caggtagaag tggatcgaga
     4561 accgccggcg ctcggcgtac tcgtacggtt tcatcccgca ctcgtagggc gtctggtcga
     4621 ccacgctgcc gcgcacgcgg ccgatcaggt gcgagagcgc gagcagcgca ccggccatcc
     4681 cgccggcgat cagcaggacc aacaggaccg tcagccggct gtcagacatc gacctctccg
     4741 gaaacgctct ggcatggtgt gggagcagcg cgccaccgac gaaacgccgg tcgtggaatc
     4801 taacacaacg ccgtaaaacg tgtcaacttg gcccatgacc gagaaggtgt tgtttttcaa
     4861 cgagttgaga gtgacgaacc ccccgtctgg caggcaaacg aaagggaggc ccggaggcct
     4921 ccctgcgcga gcattcgacg ggggatcgcg gtcgttcagt cgcccttctt gcggcgttct
     4981 tcccactcct tgcgcttctt catctcttcc tcgtattcgg cctgcgctcg cgcctcggcc
     5041 gcgcgctgcg cctcttcctc gctcgcgatc tgcgcgttgt ggtctcgcac cgcgatgttc
     5101 tgcttgcaga tctcgatcgc gttgcgcacc gactcggacg gcttgatgtc gtacgatttc
     5161 tcgtaggccg tgaccgcttc ggtcagccgg ttctgctttt gcagcgagaa cccgaggctc
     5221 tcgtagatca ccgccatgcg cggcgcgcaa gcggccgcgt cgttgagcac cttctccgcg
     5281 tcggcccaac gctcctccgc gatgtaggtc ttgccgagat tgaccatcgc gtagcagtag
     5341 tccggcttct gcgacagcac cttgcggaac gcctgttcgg ccttggtgta ttcgctggcg
     5401 ccgagcgcgg cgcggccgac cagattggcc gcttcgtagt tggccggctc cacgctctgg
     5461 aacttctcgg cgtaacccat cgcctcctcg tacatcgagt ctttctgcga gtcgctcgtc
     5521 gactccgcgc cgagattgat cagggcctcc gccaacagtt ggacgctggc ggtgtcgttg
     5581 ggaacgagct tgaccgcctc gcggaagacc ggcaccgcct tgtcgtaatg gccgagtttg
     5641 tagtaggact gaccgagcgt caccagcacc gacggtttct tggcgttgat cttgcgcgcc
     5701 tgctcgagat cctcgaccgc ctcggcccac ttgcccatcg ccgcgtagca gccgccgcgc
     5761 gtcgaataga gcgcgaactt ctggttgttg tccgcggcaa gcggctcggc ggtcttcaac
     5821 gcggcgatcg ccttggcgta gtctttcttg gtgaagtgca cggtcgccag accgttgtgg
     5881 tattcgaact tgtcccc
//
LOCUS       DASPGN010000444        18855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_549938, whole genome shotgun sequence.
ACCESSION   DASPGN010000444 DASPGN010000000
VERSION     DASPGN010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 18855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18855
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549938"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..1478
                     /gene="sufB"
                     /locus_tag="VD788_12965"
     CDS             30..1478
                     /gene="sufB"
                     /locus_tag="VD788_12965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HXV77221.1"
                     /translation="MKTNETTVEQLANREYKWGFTTDIEADSVPPGLDEGTIRTISAK
                     KNEPQFLLDWRLEAFRHWQTMTEPHWAFVSYPPIDYQKIVYYSAPKRRSDRPASLDEV
                     DPQLLETYNKLGIPLKEQEMLAGVAVDAVFDSVSVATTFRGKLSELGVIFCSFSEAVQ
                     QHPELVRRWLGSVVPHTDNFFAALNSAVFTEGSFVYIPEGVRCPMELSTYFRINAANT
                     GQFERTLIVADKGSYVSYLEGCTAPMRDENQLHAAVVELVALDDAEIKYSTVQNWYPG
                     DAEGRGGIYNFVTKRGKCQGRRSKISWTQVETGSAITWKYPSCILQGDDSVGGFYSVA
                     LTNGRQQADTGTKMIHLGKNTKSTIVSKGISAGRGQNTYRGLVKISKRADGARNYSQC
                     DSMLIGDQCGAHTFPYIDVSNSSATMEHEATTSKIGEDQLFYCRQRGISTEHAISMIV
                     NGFCKEVFRELPMEFAVEAQKLLEVNLEGSVG"
     gene            1475..2260
                     /gene="sufC"
                     /locus_tag="VD788_12970"
     CDS             1475..2260
                     /gene="sufC"
                     /locus_tag="VD788_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HXV77222.1"
                     /translation="MKENDEPMLNIESLAASVDGTPILKGIDLAVNAGEVHAIMGPNG
                     SGKSTLAGVLSGREAYDVTAGSVRYDGKDLLEMAPEDRACEGVFLAFQYPVEIPGVST
                     VNFLKAAVNSKRKYRGQPELDAMDFLALLREKLSIVDMDESFLRRAVNEGFSGGEKKR
                     NEIFQMAVLDPRLAVLDETDSGLDIDALKIVADGVNSLRGPERAIVVITHYQRLLNYI
                     VPDFVHVLSDGKIVRSGDRTLAEELERKGYGWLEKAGAAPGAG"
     gene            2278..3606
                     /gene="sufD"
                     /locus_tag="VD788_12975"
     CDS             2278..3606
                     /gene="sufD"
                     /locus_tag="VD788_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HXV77223.1"
                     /translation="MNDRTKATNSFLDGFRELERVLTRNGPGWLVEARRGSRARFDQL
                     GFPTTNEEAWRNTNVSPIAQGSFRPAERATISFDRLPALARLDLGGPRVVLVDGHLDP
                     SLSRLDPLPRGLEVDGLGRLLARDPDAVQSWWSEFRAEDGSAFRALNTTLAQDGAVVR
                     TAPRTIVDHPLHVVHVATGETAARGSHNATHSRCLVSAGEASRLHVVESYVAIGAEPY
                     LTNAVTEVELADGAVLQHDRIQLESAGAYHIATVDSRQRGSTLYVARHFNFGGKLVRN
                     ELRSRLDGDGGNCTIDGLYVTRGSQHVDNHTLLDHARPHCDSRELFKGILMGRSRTVF
                     HGRIIVRQAAQKTDAKQSNPNLLLSGEALAHTRPQLEIYADDVKCTHGATIGQLDRDA
                     IFYLRSRGIDDDSARNLLTAAFAGEILDRVTIEPLRELLAAEVGRRLAAG"
     gene            3619..4887
                     /locus_tag="VD788_12980"
     CDS             3619..4887
                     /locus_tag="VD788_12980"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HXV77224.1"
                     /translation="MTRRESTAAPLPGDLTGDAPNPFRRDFPILEREVDGRPLVYLDN
                     AATTQKPGVVIQAVERFYGEICANVHRGLHRLSTEATEAFEGARAKVARFVGAARTEE
                     VVFTSGTTAAVNLVAHSFGSRFVRSGDEVLITEMEHHSNIIPWQLLCERSGARLRVVP
                     IDDDGALRLDEFERLLGPRTRIVSVAHTSNSLGTRNPIERIVAMAHAAGAVVLVDAAQ
                     AMVHQRVDVGRLGCDLLALSGHKMFGPTGIGALYGRYDLLERLPPFLGGGEMIEVVRF
                     ERSTYKAPPHRFEAGTPNIAGAIGLGAAVDYLERAGLARIAHHDARLLAYATERLLEI
                     PGLRLIGTAADKTSILSFNLPRIHSHDVGQWLDGDGIAVRAGHHCAQPVMRHFGVPST
                     VRASLVCYNTRDEVDALVRGLVKTLEVMGR"
     gene            4884..5357
                     /locus_tag="VD788_12985"
     CDS             4884..5357
                     /locus_tag="VD788_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HXV77225.1"
                     /translation="MSDLRDLYQETILDHGKNPRNFRKLEDADRCADGHNPLCGDRLT
                     VWVKLDGDRITDVTFDGNGCAISMASASMMGETLRGRSIDEVRRVFESFHELVTGGAG
                     DGESRATLGKLVVFSGVSEFPVRVKCATLAWHTLRAALERGVADDVRPVSTERAE"
     gene            5354..5674
                     /locus_tag="VD788_12990"
     CDS             5354..5674
                     /locus_tag="VD788_12990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HXV77226.1"
                     /translation="MTESAPARLREEVVAALKTCYDPEIPVDIYELGLVYDVEVSDEA
                     AVRIKMTLTSPACPVAGSLPGEVERKVRDVEGVRSAEVELVWDPPWDQEMMSDAAKVK
                     LGMF"
     gene            complement(5671..6687)
                     /locus_tag="VD788_12995"
     CDS             complement(5671..6687)
                     /locus_tag="VD788_12995"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXV77227.1"
                     /translation="MQRADLPPPISLLEPYDRSSSAVGEAAVSAPGWHGTRGRLDPPG
                     EARERRRPLFHPARGWIRRTERATSHFLTRFVFPWVPGIGIPYSGLLGRQLVLSEATI
                     ALAGLPAAFERMRILLITDIHAGPFVDPPTLDELFERLCRLEPDLILIGGDVSSSSIR
                     EIDRSRAAFERLAAPLGVWAVLGNHDHYTGRPAAVIERLASYGIRTLSNRSVALLRDG
                     ERLSLAGVDDLLMGKADLDAALDDAVPPVVLLSHNPDLLFAAARRGVALMLSGHTHAG
                     QIRLPGLGVLVRQSRFRLDQGRYRFRQTELVVSRGVGAVGIPWRVQCPPEVVLLTLGR
                     QPSS"
     gene            complement(6699..7187)
                     /locus_tag="VD788_13000"
     CDS             complement(6699..7187)
                     /locus_tag="VD788_13000"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HXV77228.1"
                     /translation="MSGEGAGERLQQLWDRIAGYPGGRWLFSRLVGRIIPYSGTIAAS
                     IVELAPGHARVRIRDRRSVRNHLHSIHAIALLNLGELTTGLALNCGLSSSLRTILVHV
                     AMDYEKKARGVITAECRCVQPDDRNEQELPVTGELRDGTGDVVAVVRAVWRVGPTAPA
                     AG"
     gene            complement(7291..8337)
                     /locus_tag="VD788_13005"
     CDS             complement(7291..8337)
                     /locus_tag="VD788_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77229.1"
                     /translation="MSPKHEPQTSDRRLLLAIAAFVVAAAAGVALLSGLAERGAGGSE
                     GPTAPALATAPREPAPLSAEPASLERPAGPALDENAARFVVDPDADPWQEGVRAYHAG
                     RYDEACAWLAAEVEARPERAEAHYLLGLAAWKSSEPERAVEALERSAVLNPSSIRTYV
                     NLARVRNEGGDYERALRDAERAVEIDPLHAGALYQKARTLANLGRNDEAVELLDEVIA
                     RDPGMGHAYNLLGLIGLREGRDRAALDALTLAAERLPDVAFVHNNLGLALERSERAVE
                     AAQAFRRAIELDAGHAAAAVSLARVQSNPPVNLEAIEVASGETSVSPVEEPRAVTDAG
                     AIEVPAGPTAPDLE"
     gene            complement(8549..9907)
                     /locus_tag="VD788_13010"
     CDS             complement(8549..9907)
                     /locus_tag="VD788_13010"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV77230.1"
                     /translation="MSTILIADDERNIRSSLSNTFQLEGYDVVTAEDGEQAVAAVERG
                     GIDLALLDLQMPRLDGLQALGRLRELKYDLPAIFLTAHGTFEKAVEAVRVLGAFDFIE
                     KPPHAEKILLSTKNALRQVALEEENRELRGLSSSKFDMIGDTSVMKQLYEQILRAAPT
                     QARVLILGENGTGKELIARALHQHSQRAAGSFVRVNCAAIPRELFESELFGHERGAFT
                     GATARRKGKFVRADGGTLFLDEVGEIPIELQPKILRALESGEVEPVGADRELRVDVRV
                     LAATNRDLEQAAADGKFRQDLFYRLQVVTLKAPPLRERKEDIPILAGRFLADACQENN
                     LPLRELTDAAVRRLSQHDYPGNVRELRNLVERLVILTTDRRIDVGAVEGCLPQPRASR
                     PAEVSLRGTLRETLEEVERVLVHKTLEGHHWRMTEVAQQLGLERSHLYKKMKALGISK
                     PE"
     gene            complement(9904..11754)
                     /locus_tag="VD788_13015"
     CDS             complement(9904..11754)
                     /locus_tag="VD788_13015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV77231.1"
                     /translation="MAAVTNGAHPTYHLGGRRRLRVTARLAVELFVLAVLITGACAWT
                     ALNLVDDTFRKQLTTELDLLVGGVNEVFDAETDRVHQLLARLKSYLDRDPELVEALFA
                     EGEARVAAVPRVRETTARLMEQYQLDYLDLLDGAGMVLSSGYPERVGVPDERFSTLLD
                     GGFQLSQIKREDQRLVITTRLDLDLGLRSLVLIGGRLIDEDLVNRLAGREAAVLYIAR
                     GAGESPRIIPSGQLLKAALGELLALGDIPAGEPVSFVVDRRTFLFKRTPLTAEAPERA
                     WVLLGVDLTRFERLHRQLVHAFLYLGAGVGLVAALAGVWIAQRTARPVRELVSALDSI
                     ARGEADYAFGVRAPDEAQELAVSFSRMHRALENQRRRSMAAERVAAWQEVARHVAHEV
                     KNPLAPIRLTVENLLRARSQAPERFDQLFREGMQTILEEVQQLSRMVSEFAEFARLPL
                     PIRREVDLADLIDRVVELYASEPGVEIGKVYPAELPPLQLDSDQLSRALKNILGNAID
                     AARDRTDTDPAARVEIRLSIEEGMAQIEVTDNGPGLAPEVERRLFEPYFTTKPHGTGL
                     GMALTYRIVIEHDGVIFAENRPDRGARIVIRLPFGAPGPQVARARKEAVG"
     gene            complement(11742..13361)
                     /locus_tag="VD788_13020"
     CDS             complement(11742..13361)
                     /locus_tag="VD788_13020"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV77232.1"
                     /translation="MRIPVVILLLSLAYAGCSNRGAVDLSPEPPTAPAPVPPPESDPV
                     QQGTTVDTHIASPEEVLPSVEPRFGGFIRFLVTDDRTGQAVIHSNVAEGLTPAGMLLS
                     WAEPRGDSTCWTLDLNPHFKFPGGAPVRATDLVALWQRSLDAVDEFGEEQWLLKSIGI
                     EPVSGDGGTVRLAGASAYSGPQQEKLVLCPSFPAPDLLERLSHPALWLVALDDAGSGP
                     GGFWTGDGRVLQRNPNYPRRVPYLDRVEFLRVSGEASLPLRLGDAHVAVVYGKVAREL
                     AEVFGDVIDPAAGAPVDEATARPGDGKSIELRRLPSWDRTYYLWMNKTKRWLNDPKFR
                     AWVADVVDRKALLELLFDGQGRVAFSLFEAVDQTAYYPEQQTRPLSQSSSPRLELVYD
                     RDDRHALTIARRFQAVLETADVALALREMDRAAIRAALRDGEIEMAVLAHHPATPDPM
                     LALLGTIWWLGPGSTEIKEALVGSASAVSADEPGQRLSAATALERKLLVDAVLIPLIR
                     LDAVLARHIGLAGLSAESHGVLRFDEAWWLP"
     gene            13499..14053
                     /locus_tag="VD788_13025"
     CDS             13499..14053
                     /locus_tag="VD788_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77233.1"
                     /translation="MRRLSIGFIVMACCFCLTAAAADRWVHIRVQNLDGEDGSISVNV
                     PLHMLESWLPTIDIDDFDDLDWEIGRHRGDSRIDLRQLAAALRDAPDGEFVRIRGRDD
                     ERVRVAKDEGYLIAEVDETNGDRVRVRLPMSVVEAIVDGGSERIDVPAVLRALADHGD
                     EDLVTVESADGTVRIWIDDRASGD"
     gene            14116..14643
                     /locus_tag="VD788_13030"
     CDS             14116..14643
                     /locus_tag="VD788_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77234.1"
                     /translation="MAKRVTAILLLLSAAGLARGGYPLPRSSEEGAARQESWRLALER
                     ADRAVSERRWDEAERLFAAVVDESEAADVSGMMRARALDGLADLHQRASRHEQAARLY
                     GEAATMWERLLGPSQPRLGLTLRNLGVCYVALGRADEARLAFERALAIFERAFGPSSA
                     EVARTRRMLDQLADS"
     gene            complement(14647..15426)
                     /locus_tag="VD788_13035"
     CDS             complement(14647..15426)
                     /locus_tag="VD788_13035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77235.1"
                     /translation="MASYPEVVPPGQVGNIDAQIDTLKLTGEVGRGVTVYSNDPTRPT
                     VFLTVHAVVVGGVVVLPEERLVLTNATEAGSKRALVVRRGVDEDGELTIGDVQLDVPW
                     LDVVVEELAEPRNMRDGAPSADAGDWVVSVSLGRAAPVGRHEANLTFGTGLERQPVVS
                     VPIVARKMAPVMLSEERLVLPRSAGEPVARGTFLLTVRRGLDPDALEVTSSPGELTTD
                     LERSGDRGYKLHLAWASSDPPAGEVTFRVGAEELRVPVAAR"
     gene            complement(15436..15690)
                     /locus_tag="VD788_13040"
     CDS             complement(15436..15690)
                     /locus_tag="VD788_13040"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HXV77236.1"
                     /translation="MVELRSPLPPCRARRLRLLSSALVLSTLAGPSMAQDAPAPGPRI
                     EFEVEQIDLGRIDRGSVVTAQFKLRNSGTSDLRIESVKPG"
     gene            15888..17261
                     /locus_tag="VD788_13045"
     CDS             15888..17261
                     /locus_tag="VD788_13045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXV77237.1"
                     /translation="MIRLPRRRLVQLAWLSSWVLFAWLLVAMSAPGRVPSDPTTAVVP
                     PSIAGPPAPTAPAEPLLRSLETTVGRRGTLAASLDRLDVPVEHRQRLVELASESLELK
                     RLDPRTGLLARIDGEGRWHSLSIRDEPERFLRIEIGGAAGAPAYEARRVELPVTTDVR
                     TLSGVIDRSVAQAVAGSPHAVPLVAAFADIFQWDVDLLVEPRKGDRVKIVYELRTLGD
                     PPDDLPEFAGTPREPGEVLAVSRILAASYLGEVAQTHAFWVEGPDGAGDYYDVDGRPL
                     RKTFLKSPLNYRRISSGFSRARRHPVTRKIVPHHGIDYAAAPGTPVVAAADGRVVSAG
                     WDGALGKAVRIRHGSEYLTVYGHLRAFARGVRRGAEIRQNEVVGYVGSTGRATGPHLH
                     YTMIHRGRPVDPRRIDNPAGEPLDASRFVELELAVRRFANGIDLDAASLASAARLRTD
                     GASGFVR"
     gene            complement(17296..18012)
                     /locus_tag="VD788_13050"
     CDS             complement(17296..18012)
                     /locus_tag="VD788_13050"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXV77238.1"
                     /translation="MDAELIRLLPLWYVVFLLSITCHEAAHAWVAYRGGDETAYLGGQ
                     VSLNPMPHVVREPFGTVLVPLISYFLFSSTSGRWMIGWASAPYDPYWEDRHPKRAALM
                     SAAGPAANLLLAVIGFVGLKVGLARGLWEIPGADLAFDRLVAQPVGLRDGSAWVGLGR
                     FCSVLFSLNLILCVFNLLPIPPMDGASVVSGISEPARRLRHRLGGSPLFGLVGLLVAW
                     NVFPYLFYPIYRVALGWLYG"
     gene            18164..>18855
                     /locus_tag="VD788_13055"
     CDS             18164..>18855
                     /locus_tag="VD788_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77239.1"
                     /translation="MDTDARASLDGAAGRQASKGALQRTLPTAPAEFLDGALRNPALG
                     NDEMRLLLRNYRVGAATLAQIGRDARWVGQGEVRRLLVQHPHVPLTIARNLLGHLSWK
                     DLLEVSVNLRVHPFVRRQAERTLELRVERMSTGERISLARRATRGVIGQLVVDDDPRV
                     ARALLDNPRLIEKNAVAIAVSPRSRPATLASLAQHAAWGQRPAVRAGLLRNPRTPVAV
                     ALKTVAALPRSSL"
BASE COUNT         3010 a         6559 c         6449 g         2837 t
ORIGIN      
        1 cacctgacga gcggcaagag cgaggagcga tgaagacgaa cgaaacgacc gtcgaacagc
       61 tcgccaatcg cgagtacaaa tggggcttca ccaccgacat cgaggcggac agcgtgcctc
      121 cgggtctcga cgaagggacc atccgcacga tctccgcgaa gaagaacgag ccgcagttcc
      181 tgctggactg gcggctcgag gcctttcgtc actggcagac gatgaccgag ccccactggg
      241 cgttcgtctc gtacccgccg atcgactacc aaaagatcgt ctactactcg gcgccgaagc
      301 gccggtcgga ccggccggcc agcttggacg aggtcgaccc gcagctgctc gagacctaca
      361 acaagctcgg catcccgctc aaggaacaag agatgttggc cggggtcgcc gtcgacgccg
      421 tgttcgacag cgtctcggtc gcgacgacct tccgcgggaa actgtcggag ctgggcgtga
      481 tcttctgctc gttctcggag gcggtccagc agcatccgga gctcgttcgc cgctggctcg
      541 gctcggtcgt tccgcacacc gacaactttt tcgccgcgct caactcggcc gtgttcaccg
      601 agggctcgtt cgtctacatc cccgagggtg tccgttgtcc gatggagctg tcgacctact
      661 ttcgcatcaa cgcggccaac accgggcagt tcgagcgaac gttgatcgtc gccgacaagg
      721 gaagctacgt cagctacctc gaaggatgca ccgcgccgat gcgcgacgag aaccagcttc
      781 acgccgccgt cgtcgagctc gtcgcgttgg acgatgccga gatcaagtac tcgaccgtgc
      841 aaaactggta cccgggcgac gccgagggcc gcggcgggat ctacaacttc gtgacgaagc
      901 gcggcaagtg ccagggccgg cgctcgaaga tcagctggac ccaggtcgag accggatcgg
      961 cgatcacctg gaagtacccg agctgcatcc tgcagggcga cgactcggtc ggcggattct
     1021 actcggtcgc gctgaccaac ggccgccagc aggcggacac cggcacgaag atgatccatc
     1081 tgggcaagaa cacgaagagc acgatcgtgt cgaagggaat ctcggccggt cgcggacaga
     1141 acacctaccg cgggttggtc aagatcagca agcgggcaga cggtgcacgg aattactcgc
     1201 aatgcgattc gatgctgatc ggcgatcagt gcggtgccca caccttcccg tacatcgacg
     1261 tgtcgaactc ctcggccacg atggagcacg aggcgacgac atccaagatc ggcgaagacc
     1321 agttgtttta ttgccggcag cgggggatct cgacggagca cgcgatctcg atgatcgtca
     1381 acgggttctg caaggaggtt tttcgcgagc taccgatgga gttcgccgtc gaggcgcaga
     1441 agctgctcga agtcaatctc gaaggaagcg taggatgaag gaaaacgacg agccgatgtt
     1501 gaacatcgag agcttggcgg cgtcggtcga cggtacaccg atcctcaagg gaatcgacct
     1561 cgcggtcaac gcgggcgaag tccacgcgat catgggcccc aacgggtcgg ggaagagcac
     1621 gctggccggc gtcctgtccg ggcgcgaggc gtacgacgtc accgcgggca gcgtccgcta
     1681 cgacggcaag gacctgctcg agatggcgcc cgaggaccgc gcgtgcgagg gagttttcct
     1741 cgcgttccag tacccggtcg agatcccggg ggtcagcacg gtcaacttcc tcaaggccgc
     1801 ggtcaattcg aagcgcaagt accgcggaca gccggagctc gacgcgatgg acttcctggc
     1861 actgttgcgc gagaagctga gcatcgtcga catggacgag tcgttcctga gacgtgcggt
     1921 caacgaggga ttctccggcg gcgagaagaa acgaaacgaa atcttccaga tggccgtgct
     1981 cgacccgcgg ctggcggtgt tggacgagac cgactcgggc ctcgacatcg acgcgctgaa
     2041 gatcgtcgcc gacggcgtca actcgctgcg cggccccgag cgcgcgatcg tggtgattac
     2101 gcactatcag cggttgttga actacatcgt gcccgacttc gtccacgtgc tgtcggacgg
     2161 caagatcgtg cgctccggcg atcgcacgct ggccgaggag ctcgagcgca agggctacgg
     2221 gtggttggag aaggccggcg ccgctcccgg cgctggctga cgccgcgagc aaccacgatg
     2281 aacgaccgaa cgaaagcgac gaacagtttt ctcgacggct tccgcgagct tgaacgggta
     2341 ttgacccgaa acggaccggg ctggctggtc gaggcgcggc gcggcagccg ggcacgtttc
     2401 gaccaattgg gcttcccgac gacgaacgag gaggcctggc gcaacaccaa cgtctcgccg
     2461 atcgcgcagg gttcgttcag gccggccgag cgcgcgacga tctcgtttga tcggctgccg
     2521 gctctcgcgc ggctcgatct cggcggcccg cgcgtggtgc tggtcgatgg tcatctcgac
     2581 ccgagcctgt cgcggctcga cccgctgccg cggggcctcg aggtcgacgg cctcggtcgg
     2641 ctgttggccc gcgacccgga tgccgtccag agctggtgga gcgagtttcg cgcggaggac
     2701 ggcagcgcgt ttcgcgcgct gaacacgacg ttggcgcagg acggcgccgt ggtgcggacc
     2761 gccccgcgca cgatcgtcga ccacccgctc cacgtcgtgc acgtggcgac cggcgagacc
     2821 gccgcgcgtg gatcgcacaa cgcgacgcac agtcgctgct tggtctccgc cggcgaggcc
     2881 agccggttgc atgtcgtcga aagctacgtc gcgatcggcg cggagcccta tctgaccaat
     2941 gccgtcaccg aggtcgagct ggccgacggg gcggtgctgc agcacgatcg aatccagctt
     3001 gaatcggccg gtgcctacca catcgcgacc gtcgattcgc gtcagcgtgg ctccacgctc
     3061 tacgtcgccc ggcacttcaa cttcggcggc aagctcgtgc gcaacgagct gcgcagcagg
     3121 ctcgacggcg acggcggcaa ctgcacgatc gacggcctgt acgtgacccg cgggtcacag
     3181 cacgtcgaca accacacctt gctggaccac gcccgcccgc actgtgacag ccgcgagctg
     3241 ttcaagggca tcctgatggg acgatcgcgg accgtgtttc acggccgcat catcgtccgt
     3301 caggcggcgc agaagaccga cgccaaacag tccaatccga acctgctgct gtcgggagag
     3361 gcgctggccc atacacgccc gcagctcgag atctacgcgg acgacgtcaa gtgcacgcac
     3421 ggggcgacga tcggccagct cgaccgggat gcgatcttct acctgcgcag tcgcgggatc
     3481 gacgacgact ccgcccgcaa cctgctgacc gcagcgtttg ccggcgagat tctcgaccgc
     3541 gtgacgatcg agccgctgcg cgagctgctg gcggccgagg tcgggcgccg gctcgccgcc
     3601 ggataggagt gccgtagcgt gacgcgccgc gagtcgactg ccgcaccgct acccggtgac
     3661 ctcaccggtg acgcgccgaa tccgttccgc cgcgactttc caatcctcga gcgagaagtg
     3721 gacggccgac cactggtcta cctggacaac gcggcgacga cgcagaagcc cggagtcgtg
     3781 atccaagcgg tcgagcgctt ctacggcgag atctgcgcca acgtccatcg tggcctccac
     3841 cgcttgagca ccgaggcgac cgaggcgttc gagggggcgc gcgcgaaggt cgcacgtttc
     3901 gtcggtgccg ctcgcacgga ggaggtcgtc ttcaccagcg gcaccaccgc tgcggtcaat
     3961 ctggtcgcgc actcgttcgg cagccggttc gtccgcagcg gcgacgaagt gctgatcacc
     4021 gagatggagc accactccaa tatcatcccg tggcagctcc tgtgcgagcg gagcggggca
     4081 cggctgcgcg tcgtgccgat cgacgacgac ggcgccctcc gcctggacga gttcgagcgt
     4141 ctgctcggac cacgcacacg catcgtgtcg gtcgcccaca cctcgaactc gctcggcacg
     4201 cgcaacccga tcgagcggat cgtcgcgatg gcccacgcgg caggcgccgt cgtgctggtc
     4261 gacgccgcgc aggcgatggt gcaccagcgg gtcgatgtcg gccgcctggg ctgtgacctg
     4321 ctcgcgctgt ccgggcacaa gatgttcggg ccgaccggga tcggcgcgct ctacggacgt
     4381 tacgacctgc tggaacggct gccgcccttt ctcggcggcg gcgagatgat cgaggtcgtg
     4441 cggttcgagc gctcgacgta caaggcgcca ccccaccggt tcgaggcggg cacgccgaac
     4501 atcgccggcg cgatcgggct cggcgccgcc gtcgactatc tcgagcgagc ggggctcgcg
     4561 cgcatcgcgc accacgacgc gcgactgttg gcctacgcca ccgagcggct gctcgagatc
     4621 cccggcctgc ggttgatcgg taccgcggcg gacaagacga gcatcctgtc gttcaatctc
     4681 ccgcgcatcc actcgcacga cgtcgggcag tggctcgatg gggacgggat cgccgtccgc
     4741 gccggccacc actgcgccca gccggtgatg cggcatttcg gcgtgccgtc gaccgtgcgg
     4801 gcctcgctgg tgtgctacaa cactcgcgac gaggtcgatg cgttggttcg tggtctggtc
     4861 aagacactcg aggtgatggg tcgatgagcg atctgcggga cctctaccag gagacgattc
     4921 tggaccacgg gaagaacccg cgcaattttc gcaagctgga ggacgcggac cgttgtgccg
     4981 acggacacaa cccgctgtgc ggggaccggt tgacggtgtg ggtcaagctc gacggggacc
     5041 gcatcaccga cgtgacgttc gacggcaacg ggtgcgcgat ttcgatggcc tcggcgtcga
     5101 tgatgggcga gacgctccgc gggcgctcga tcgacgaggt ccggcgcgtg ttcgagtcgt
     5161 tccacgagct cgtcaccggc ggggccggag acggtgagtc gcgcgcgacc ctgggcaagc
     5221 tggtcgtctt ctccggcgtc agcgagttcc cggtccgcgt caagtgcgcg accttggcct
     5281 ggcacacctt gcgcgccgcg ctcgagcgcg gcgtggccga cgacgtccgc ccggtgagca
     5341 cggagcgcgc ggaatgacgg agtcggcccc cgctcggctg cgcgaggagg tcgtcgcggc
     5401 gttgaagacc tgctacgacc ccgagatccc ggtcgacatc tacgagctcg gcttggtcta
     5461 cgacgtcgag gtgagcgacg aggcggcggt ccggatcaag atgaccctga cctcacccgc
     5521 ctgtccggtt gccggatcgc tcccgggcga ggtcgagcgg aaggtgcgcg atgtcgaggg
     5581 cgtccgctcg gccgaggtcg agctcgtctg ggacccgccg tgggatcagg agatgatgag
     5641 cgacgcggcg aaggttaagc tcgggatgtt ctagctcgac ggctgccggc cgagcgtgag
     5701 caacacgacc tccggcgggc attgcacacg ccacgggatg ccgaccgccc cgacgccgcg
     5761 actgacgacc agttccgttt gccgaaagcg atagcggccc tggtcgagac ggaatcggct
     5821 ctggcgcacc aacaccccca accccggtag ccggatctgg ccggcgtgcg tatgaccgga
     5881 cagcatcagt gccactccgc gccgtgccgc ggcgaacaac aggtcggggt tgtgcgagag
     5941 tagcacgacg ggcggtacgg catcgtcgag cgccgcgtcg agatcggcct ttcccatcaa
     6001 cagatcgtcg acgccggcca ggctcagtcg ctcgccgtcg cggaggagtg cgacggaccg
     6061 gttcgacagc gtacggatgc catagctcgc cagccgctcg atgacggccg ccggccgtcc
     6121 tgtgtagtgg tcgtggttcc ccaggacggc ccagacgccg agcggcgcgg ccaggcgctc
     6181 gaacgccgcg cgcgagcggt cgatctcccg gatcgacgac gacgagacgt cgccgccgat
     6241 caggatcagg tccggctcca agcggcacag gcgctcgaac aactcgtcga gcgttggcgg
     6301 atcgacgaac ggtccggcgt gtatgtccgt gatcaacagg atccgcatcc gttcgaaggc
     6361 ggcgggaaga ccggcgagcg cgatcgtcgc ctcggaaagc acgagctgcc tccccagcag
     6421 cccgctgtac gggatgccga ttccggggac ccacggaaac acgaaccgcg tgaggaagtg
     6481 gctcgtcgcc cgttcggtcc gccggatcca gccgcgggcg ggatgaaaca gcggccggcg
     6541 tcgctcgcgt gcctccccgg gtgggtcgag ccgaccgcgc gtgccatgcc aaccgggcgc
     6601 ggaaacggcg gcctccccga ccgccgacga cgaacgatcg tacggctcga gcagcgaaat
     6661 cggcggcgga aggtcggctc gctgcatcga ggacccgctc agccggccgc gggcgcggtc
     6721 gggccgaccc gccataccgc ccgcaccacg gcgacgacgt cgccggtccc gtcgcgcaac
     6781 tcgcccgtca ccggcagctc ctgctcgttc cgatcgtcgg gctgcacgca gcgacactcg
     6841 gcggtgatca cgcctcgtgc cttcttctcg tagtccattg cgacgtggac caggatcgtc
     6901 cgcagtgacg acgacagccc gcagttcaac gccagaccgg tcgtcagttc accgagattc
     6961 aacagcgcga tggcgtggat cgagtgcaag tggttgcgca cgctccggcg atcgcggatg
     7021 cggacgcgtg cgtgacccgg cgcgagttcg acgatcgagg ccgcgatcgt gccgctgtac
     7081 gggatgatcc ggccgacgag tcgactgaac aaccaccgtc cgcccgggta gccggcgatc
     7141 cggtcccaca gctgctggag tcgctcgccc gcgccttcgc cgctcatcgg tactcccgtt
     7201 cgtgctcgtc ggggctccat cctagcgccg agacgcggcg ggggcatccg ggaacgggcc
     7261 gacgaccccg gataccccgc gccgccaccg tcactcgaga tcgggagcgg tgggcccggc
     7321 cggcacctcg atcgcgccgg catcggtcac ggcacgcggc tcctcgaccg gcgagacgga
     7381 ggtctcaccg ctcgcgacct cgatcgcttc caggttgacc ggcgggttgg actgaacgcg
     7441 cgccagactg accgcggcgg cggcatgccc ggcgtccagc tcgatcgcgc gccggaacgc
     7501 ctgggccgcc tccacggctc gctcgctgcg ctcgagcgcc agaccgagat tgttgtgaac
     7561 gaacgcgacg tccggcagcc gttcggcggc gagcgtgagc gcgtcgagcg cggcccgatc
     7621 ccgcccctcg cgcaacccga tcaggccgag gaggttgtag gcgtgcccca tccccggatc
     7681 acgcgcgata acctcgtcga gcagttcgac ggcctcgtcg ttccggccga ggttggccag
     7741 cgtgcgggct ttctgataga gcgctcccgc gtgaagcgga tcgatctcga ccgcacgctc
     7801 ggcgtcgcgg agcgcgcgct cgtagtcgcc gccctcgttc cgtacccggg ccaggttgac
     7861 gtaggtccgg atcgaactcg ggttcagcac ggcggatcgc tcgagggcct cgaccgcccg
     7921 ctcgggctcg gacgatttcc acgccgccag acccaacagg tagtgggcct cggcgcgctc
     7981 cggccgggcc tcgacctcgg cggcgagcca ggcgcacgcc tcgtcgtaac gcccggcgtg
     8041 gtaggcccgt acgccttcct gccacggatc ggcgtcggga tcgacgacga agcgagccgc
     8101 gttctcgtcc aacgcaggtc ctgcaggcct ctcgagcgag gccggctcgg ccgacagggg
     8161 tgcgggctcg cgcggagcgg tggccaaggc cggcgccgtc ggtccttcgc tgcctccggc
     8221 tccccgttcg gcgagaccgg acaacagcgc gacgccggcg gcggccgcga cgacgaaggc
     8281 cgcgatggcg agcaacagcc gccgatcgct ggtttggggt tcgtgtttgg ggctcatgtc
     8341 gattctccac ccgccgccgg ccggcggccg cggtttgcgg tcgaatgcgg gtcccggctc
     8401 gacccgtcgc ccccgatcga tcgacccagc gagaacgatg ccagtttgac gtgcaccaaa
     8461 gtgctcgttt ctgatcatac aagacactat ggatgtgtgg tttagagcga atgatgagtc
     8521 agaattgaca cgcgatcgaa ggtccgcgct attccggctt gctgattccg agcgccttca
     8581 ttttcttgta caggtggctg cgctcgagcc cgagttgctg cgccacctcg gtcatccgcc
     8641 aatggtgacc ctcgagcgtc ttgtgtacca gcacgcgttc gacttcctcg agcgtctcgc
     8701 gcagcgttcc acgcagggag acctcggcag gccgtgacgc tcgcggctgg ggcaggcaac
     8761 cctcgacggc gccgacgtcg attcgacgat cggtcgtcag gatcaccagt cgctcgacca
     8821 ggttacgcag ctcccgcaca ttgcccggat agtcgtgctg gctcagccga cgcaccgcgg
     8881 cgtcggtcag ctcgcggagc ggcaggttgt tctcctggca cgcgtcggcc aggaaccgcc
     8941 cggcgaggat cgggatgtcc tccttgcgct cgcgcagcgg cggcgccttg agcgtcacca
     9001 cctgcagccg gtagaacagg tcctgccgga acttcccgtc tgccgccgcc tgctcgagat
     9061 cgcggttggt cgcggccagc acccgcacgt cgacccgcag ctcgcgatcg gcgccgaccg
     9121 gctcgacctc tcccgactcg agcgcacgca ggatcttggg ttgcagctcg atcgggatct
     9181 cgccgacctc gtcgaggaag agcgttccac cgtcggcccg cacgaacttc cccttgcgcc
     9241 gggcggtcgc cccggtaaac gcgccccgct cgtgtccgaa caactccgat tcgaacagct
     9301 cgcgcgggat cgccgcgcaa ttgacccgca cgaacgaacc ggccgctcgc tgcgagtgct
     9361 ggtgcaacgc ccgcgcgatc agctccttgc cggtcccgtt ctcgccgagg atcagcaccc
     9421 gcgcctgcgt cggcgctgcc cgcaggatct gctcgtagag ctgcttcatc accgaggtat
     9481 cgccgatcat gtcgaacttc gacgagctca aaccccgcag ctctcggttc tcctcctcga
     9541 gcgcgacctg ccgcagggcg ttcttggtgc tgagcaggat cttctccgcg tggggcggct
     9601 tctcgatgaa gtcgaaggcg ccgagaacgc ggaccgcctc gaccgccttt tcgaaggtgc
     9661 cgtgcgcggt gaggaagatc gccggcaggt cgtacttgag ctcgcgcaag cgaccgagcg
     9721 cctgcagccc gtcgagccgt ggcatctgca ggtcgagcag cgcgagatcg atcccgccgc
     9781 gctcgaccgc agcgaccgcc tgctcgccgt cctcggcggt cacgacgtcg tagccttcga
     9841 gctggaaggt gttcgacaga ctggagcgga tattccgctc gtcgtcggcg atcaggatcg
     9901 tgctcatccc acggcctctt tccgcgcgcg cgcgacttgc ggtccgggtg cgccgaacgg
     9961 cagacggatg acgatccgcg cacccctgtc cggacggttc tcggcgaaga tcacgccatc
    10021 gtgctcgatc acgatacgat aggtcaacgc cattccgagt ccagtcccgt gcggtttggt
    10081 ggtgaagtag ggctcgaaca accgccgctc gacttcgggc gccagccccg gtccgttgtc
    10141 cgtcacctcg atctgcgcca tcccctcctc gatcgacaaa cggatctcca cccgggcggc
    10201 gggatcggtg tcggtccggt cgcgtgccgc gtcgatcgcg ttccccagga tgttcttcag
    10261 cgcgcgcgac agctggtcgg aatcgagctg gagcggcggc agctcggcgg ggtacacctt
    10321 gccgatctcc actcccggtt ccgaggcgta cagctcgacg acgcgatcga tcagatcggc
    10381 gaggtcgacc tcgcggcgga tcggcagcgg cagacgggcg aactcggcga actcgctgac
    10441 catgcgactc agctgctgga cctcctcgag gatcgtctgc atcccctcgc ggaacagctg
    10501 gtcgaagcgc tcgggtgctt ggctccgcgc acgcagcaga ttctccacgg tcaaccggat
    10561 cggggccaac gggttcttga cctcgtgagc gacgtggcgc gccacctcct gccatgccgc
    10621 cacgcgctcg gcggccatcg aacggcggcg ctggttctcg agcgcgcggt gcatccgcga
    10681 gaacgagacc gcgagctcct gcgcctcgtc cggggcgcgg acgccgaagg cgtagtcggc
    10741 ctctccgcgg gcgatgctgt cgagcgccga aaccagctct ctgaccggtc gcgccgtgcg
    10801 ttgcgcgatc cagacgccgg ccagcgccgc gaccagcccg acgcccgcgc ccaggtaaag
    10861 gaatgcgtgg accagctgcc ggtgcaggcg ctcgaaccgc gtcaggtcga cgccgagcaa
    10921 gacccaggcg cgctccgggg cctcggccgt caacggggtg cgcttgaaca ggaaggtacg
    10981 gcggtcgacg acgaagctga ccggttcgcc ggcaggtatg tcgccgaggg cgagcaactc
    11041 gcccagggcg gccttcagca gctggccgga tgggatgatc ctcggcgact cgccggcgcc
    11101 gcgggcgatg tagagcacgg cggcctcgcg tccggccagg cggttgacca agtcttcgtc
    11161 gatcaaccga ccgccgatca gcacgagcga gcgcaagccg aggtcgagat cgaggcgtgt
    11221 cgtgatcacc aagcgttgat cctcccgctt gatctggctc agctggaacc cgccgtccaa
    11281 cagcgtcgag aagcgttcgt cgggcacgcc gacgcgctcc ggatatccag acgagagcac
    11341 catgccggcg ccgtcgagca ggtcgagata gtccagttga tactgctcca tcagccgggc
    11401 cgtcgtctcc cgtacgcgcg gcaccgcggc gacccgcgct tcgccctcgg cgaacaatgc
    11461 ctcgacgagc tcggggtcgc gatcgaggta actcttgagc cgcgccaaca gctggtggac
    11521 ccggtcggtc tcggcgtcga acacctcgtt gacgcccccg acgagcagat ccagctccgt
    11581 cgtgagctgc ttgcggaagg tgtcgtcgac caggttgagc gcggtccagg cgcaggcgcc
    11641 ggtgatcaac acggcgagaa cgaaaagctc gacggccaag cgcgccgtca cgcgtagcct
    11701 gcggcggccg ccgaggtggt aggtcggatg ggccccgttc gtcacggcag ccaccacgct
    11761 tcgtcgaacc ggagcacgcc gtggctttcg gccgacaggc cggcgagccc gatgtggcgc
    11821 gccagcaccg cgtcgagccg gatcagcggg atgagcaccg cgtctaccag cagcttgcgc
    11881 tcgagcgcgg tcgcggccga caggcgctga ccgggctcgt ccgcggagac cgccgaggcc
    11941 gagccgacga gcgcctcttt gatctccgtc gatcccgggc cgagccacca gatcgttccg
    12001 agcagggcca gcatcgggtc cggcgtggcg ggatggtggg ccagcaccgc catttcgatc
    12061 tcaccgtcgc gcaacgccgc tcggatcgcg gcgcggtcca tctcgcgcaa tgcgagcgcc
    12121 acgtcggccg tttcgagcac cgcctggaac cgccgggcga tcgtcagcgc atgccggtcg
    12181 tcccggtcgt agaccagctc gagacgcggg gagctcgatt gcgagagcgg gcgtgtctgc
    12241 tgctccgggt agtaggccgt ctggtcgacc gcttcgaaca aggagaaggc gacgcgtccc
    12301 tggccgtcga acaacagctc gagcaacgcc ttccgatcga cgacatcggc gacccaggcg
    12361 cggaacttcg ggtcgttcag ccagcgcttc gtcttgttca tccacaggta gtaggttcga
    12421 tcccaactcg gcaggcgccg gagctcgatc gacttcccgt cgcccggccg agcggtcgcc
    12481 tcgtccaccg gagcgcccgc ggccgggtcg atcacgtcgc cgaacacctc cgcgagctcg
    12541 cgcgccacct tgccgtagac caccgcgacg tgggcatcgc ccagccgcag cgggagcgac
    12601 gcttcgcccg acacccgcag gaactcgacc cggtcgagat aggggacgcg gcgcgggtag
    12661 ttcgggttgc gctgcaacac ccgaccgtcc ccggtccaga agccgccggg accggagccc
    12721 gcatcgtcca gggcgacgag ccacagcgcg ggatgcgaca gccgttcgag caaatccggc
    12781 gccggaaacg acgggcacag cacgagcttc tcctgctgcg gtccgctgta ggcgctcgca
    12841 ccggcgagcc ggacggtgcc gccgtcgccc gacaccggct cgatcccgat gctcttgagc
    12901 agccactgct cctcgccgaa ctcgtcgacc gcgtcgagcg agcgctgcca gagcgcgacc
    12961 agatccgtcg cgcgtaccgg ggcgccaccg gggaacttga aatgcgggtt caggtccagc
    13021 gtccaacagg tgctgtcccc gcgcggctcc gcccagctga gcagcatccc cgccggagtc
    13081 agtccctcgg cgacgttcga gtggatcacg gcctggcccg ttcgatcgtc ggtgacgagg
    13141 aagcggatga acccgccgaa acgcggctcg acgctcggca ggacctcttc gggcgacgcg
    13201 atgtgggtgt cgaccgtcgt cccctgctgg accgggtcgc tctccggcgg cggcaccggt
    13261 gccggcgcgg ttggcggctc gggcgagagg tcgaccgccc cgcgattcga gcaaccggcg
    13321 taagccagtg acaacaagag gattacgact gggatccgca ttcggttgga tcaagctacc
    13381 acgcgctcgt tcgcggtggc aacggcggac ggggtgggtg caactttccg ccggttggcc
    13441 cgttctcagg tcgacgagcc ggtcgcgtgc ggtgaccggc cgcgaacggg aggtcccgat
    13501 gcgaaggctc tcgatcggtt tcatcgtgat ggcgtgctgc ttctgtctca ctgcggcggc
    13561 cgcggatcgc tgggtgcaca tccgcgtcca gaatctcgac ggggaggacg ggtcgatcag
    13621 tgtcaacgtc ccgctccaca tgctggagtc gtggctgccg acgatcgaca tcgacgactt
    13681 cgacgacctg gattgggaga tcggtcgcca ccgcggagac tcccgcatcg acctgcgcca
    13741 gctggccgcc gcgctgcgcg atgcgcccga cggtgaattc gtccgcatcc ggggccggga
    13801 cgacgagcgg gtccgtgtgg cgaaggacga aggctatctg atcgccgagg tggacgaaac
    13861 gaacggcgac cgcgtccgcg tcaggctgcc gatgagcgtc gtcgaggcga tcgtcgacgg
    13921 cgggtccgag cggatcgacg tccccgcggt gctgcgggcg ctggccgacc acggcgacga
    13981 ggatctggtc accgtcgaat cggcggacgg cacggttcgc atctggatcg acgatcgtgc
    14041 ttcgggcgac tgacgccggc ggagtccgtc cgcggcttgt cgcgagccgt cggccacctc
    14101 atattgtcga tcgtgatggc caagcgtgta acggcgatcc tgttgctgct gtccgcggcc
    14161 ggcctggccc ggggcggata cccgttgccg cgaagctccg aggaaggggc ggcgcgacag
    14221 gagtcgtggc ggctggcgct cgagcgtgcc gaccgcgcgg tctccgagcg acgctgggac
    14281 gaagcggagc gactgttcgc ggccgtcgtc gacgagtcgg aagcggccga cgtgtccggc
    14341 atgatgcggg cccgcgcgct cgacggcctg gccgatctgc accaacgggc gtcgcgtcac
    14401 gagcaggcgg cgcgcctgta cggcgaggcc gcgacgatgt gggagaggct gctcggcccg
    14461 tcccagccgc gcctcggcct gaccctgcgc aacctcggcg tctgctacgt cgcgctcgga
    14521 cgcgcggacg aggcccggct cgccttcgag cgcgcgctcg cgatcttcga gcgcgccttc
    14581 ggaccgtcct cggccgaggt cgcgcggacg cgccggatgc tcgaccagct tgccgactcg
    14641 tgaccgttac cgcgcggcca ccgggacgcg aagctcctcg gcgcccacgc ggaacgtgac
    14701 ctctcccgcg ggcgggtcgc tcgaggccca cgcgagatgc agcttgtagc cgcggtcacc
    14761 ggaacgctcc agatcggtcg tcagctctcc ggggctcgaa gtgacctcga gcgcatccgg
    14821 atcgagcccg cggcgcacgg tcaacaggaa ggttccgcgc gccaccggtt cgccggccga
    14881 acgcggcagc accagccgct cctccgacag catcaccggc gccatcttcc gggccacgat
    14941 cggcacgctg accaccggtt gccgttcgag cccggtgccg aaggtcaggt tcgcctcgtg
    15001 gcgaccgacg ggcgccgcgc ggccgaggga gacggaaacc acccagtcgc cggcgtccgc
    15061 cgacggcgct ccgtcccgca tgttgcgcgg ctcggccagc tcttcgacga cgacatcgag
    15121 ccacggaacg tccaactgga cgtcgccgat cgtcagttcg ccgtcctcgt cgacgccgcg
    15181 gcggacgacc agggcccgct tgctgcccgc ctcggtcgcg ttggtcagca cgagacgctc
    15241 ctcgggcagc acgaccacac caccgacgac gaccgcgtgg accgtcagga acaccgtcgg
    15301 ccgggtcgga tcgttggagt agacggtcac gccccgtccg acttcgccgg tcagcttcag
    15361 cgtgtcgatc tgcgcgtcga tgtttcccac ctgcccgggt gggacgacct cggggtacga
    15421 ggccaccgcg cagcctcagc cgggtttgac gctctcgatg cgcagatcgg aggtaccgct
    15481 gttgcgcagc ttgaactgcg ccgtcacgac ggagccgcga tcgatccgcc cgagatcgat
    15541 ctgctcgacc tcgaactcga tgcgcggtcc gggtgccggc gcgtcttgcg ccatgctcgg
    15601 gcccgccagc gtcgacaaca cgagcgccga cgacagcagg cgaaggcgtc gggcgcggca
    15661 gggagggagc ggggaacgaa gctcgaccat cggatccatc ctcgtcgttg ttgcggtcgt
    15721 ttctcgcgcg acccggtctc taccgggtgg gtcatgatac ggcgagccga cttcgctgcc
    15781 cagcgccgtc tgtcgtattg tatgctgtcg ggcgtgttgc cggcgaggaa ggctgcccgc
    15841 gcggcgcgcc tcttcgcctt cccgtgcgag tggagtgccc gttgctcgtg atccgactgc
    15901 cccgccgccg cctcgtccag ctggcgtggt tgtcgtcgtg ggtgctgttc gcctggctgt
    15961 tggtcgcgat gagcgccccc ggacgcgtac cgtccgaccc gacgaccgcc gtcgttccgc
    16021 cgtcgatcgc gggaccaccc gcgcccaccg cgcccgcgga accgctgctg cgttcgctcg
    16081 agacgaccgt cgggcggcgc ggaacgctgg cggcgagcct cgaccgcctc gacgtccccg
    16141 tcgaacaccg ccagcggctg gtcgagctcg cgtccgaatc gctcgagctg aagcggctcg
    16201 acccgcgcac cgggctgctc gcgcgcatcg acggagaggg acgctggcac agcctgtcga
    16261 tccgcgacga gcccgagcgc ttcctgcgga tcgagatcgg cggcgcggcc ggtgcgccgg
    16321 cctacgaggc gcgtcgggtc gagctgccgg tcacgaccga cgtgcgcacg ctcagcggcg
    16381 tgatcgaccg ctcggtggcc caggcggtcg ccggctcgcc tcacgcggtc ccgctcgtcg
    16441 ccgcgttcgc cgacatcttc cagtgggacg tcgatctgct cgtcgagccg aggaagggcg
    16501 atcgcgtcaa gatcgtctac gagctacgaa cgctgggcga tccacccgac gacctgccgg
    16561 agttcgcggg cacgccgcgc gagccgggcg aggtgctggc cgtctcacgg atcctcgccg
    16621 cgagctatct cggcgaggtc gcgcagacgc atgcgttctg ggtcgagggt ccggacggcg
    16681 ccggcgacta ctacgacgtc gacggccggc cgctgcgcaa gacctttctc aagagtccgc
    16741 tcaactaccg gcgcatcagc agcggcttct cccgcgcgcg acgccatccc gtcacgcgca
    16801 agatcgtccc gcaccacggg atcgactacg cggcggcccc gggaacgccg gtcgtcgcgg
    16861 cggccgacgg tcgggtcgtc tccgccggct gggacggcgc gctgggcaaa gccgtccgca
    16921 tccgtcacgg cagcgagtac ctgaccgtct acggtcatct gcgcgccttc gcgcgcgggg
    16981 tgcgtcgcgg agccgagatc cggcagaacg aggtcgtcgg ttacgtcgga tcgacgggac
    17041 gcgcgaccgg gcctcatctg cactacacga tgatccaccg ggggcggccg gtcgatccgc
    17101 gccgcatcga caatcccgcc ggcgagccgc tcgatgcctc gcgtttcgtc gagctcgagc
    17161 tagccgtccg tcgcttcgcc aacggaatcg atctcgacgc cgcgtcgctc gcctcggcgg
    17221 cccgactgcg gaccgacggc gcgagcggct tcgtccgcta gcctgtcggg gtaacgcccg
    17281 cgcctcggcc ggcgatcatc cgtagagcca cccgagggcg acgcggtaga tcggataaaa
    17341 cagataggga aagacgttcc acgcgaccag cagtccgacc aacccgaaca gcggactgcc
    17401 gccgagtcga tggcgcagcc ggcgggccgg ctcggagatt ccgctgacga ccgacgcgcc
    17461 gtccatcggc gggatcggca gcaggttgaa gacacacagg atcaggttga ggctgaacag
    17521 caccgagcag aaccgcccga ggccgaccca ggccgatccg tctcgcaacc cgaccggctg
    17581 ggcaaccaac cgatcgaacg cgagatcggc tccgggaatc tcccacagac ctcgggccag
    17641 gccgaccttg agcccgacga aaccgatgac ggcgagcagc agattggccg ccggtcccgc
    17701 cgccgacatc agcgccgcgc gtttcggatg ccggtcctcc cagtacggat cgtacggggc
    17761 gctggcccag ccgatcatcc accgccccga cgtggaactg aaaaggaagt agctgatcag
    17821 cgggacgagc accgttccga acggctcccg cacgacgtgg ggcatcgggt tcaggctgac
    17881 ctggccgccg agataagccg tctcgtcccc cccgcggtac gcgacccagg catgggccgc
    17941 ctcgtggcag gtgatcgaca ggaggaagac cacgtaccac agcggcagca gccggatcag
    18001 ctcggcatcc acgacggcgt tctccccggg agcggggtcg gacccggccc ccgatgctgg
    18061 caacccggcg ggccggccac ccgggcaggg cgcgacggcc gccgaaacgc cgcgtcggcc
    18121 cgcagcggac cccgaagcgt cacggaaact atatattggc ccggtggaca ccgatgcccg
    18181 agcctcgctc gacggcgccg ccggacgcca ggcgtccaag ggcgcactcc agaggaccct
    18241 gccgacggca ccggccgagt tcctcgacgg cgcgttgcgc aacccggccc tcggcaacga
    18301 cgagatgcgg ctgctcctgc gcaactaccg tgtcggggcg gcgacgttgg cccaaatcgg
    18361 ccgcgacgcg cgctgggtcg gccaagggga ggtgcggcgt ctgctcgtcc agcaccccca
    18421 cgtcccgctg acgatcgccc gcaatctcct cggtcacctg tcctggaagg acctgttgga
    18481 ggtcagcgtc aacctccgcg tccacccttt cgtccggcgg caggccgagc ggacgctcga
    18541 gctccgcgtc gaacggatgt caaccggcga gcgcatctcg ctggcgcgac gcgcgacgcg
    18601 cggcgtgatc gggcagctcg tcgtcgacga cgacccgcgc gtcgcccgcg ccctgctcga
    18661 caacccgcgt ctgatcgaga agaacgcggt cgcgatcgcc gtgtcgccac gaagccggcc
    18721 cgcaacgctc gcgtcgctcg cgcaacacgc ggcgtggggc cagcggcccg ccgtccgggc
    18781 gggcctgctg cgcaacccgc gcaccccggt ggcggtcgcg ttgaagaccg tcgccgcgct
    18841 gccgcggtcg agcct
//
LOCUS       DASPGN010000445         5260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_198433, whole genome shotgun sequence.
ACCESSION   DASPGN010000445 DASPGN010000000
VERSION     DASPGN010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198433"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="VD788_13060"
     CDS             complement(<1..1473)
                     /locus_tag="VD788_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HXV77240.1"
                     /translation="MNGIASSTFPRLRLARATRTTLLAAALLLPLAAAHGQESQPETA
                     PVVLDGVELFRLRGTSGYPATERAADVRHRIITVGRDRSIDPGALRVEASDFGPRITV
                     GDGSLTIVVASESDAALEALSQHELAQLHLRSIRVAIAEYRELRSPRRLLRSGIWVVV
                     ATVLLVVLWILVGWTFRRLDRLIGRRLEAGVERLRIQSFRLLSAERTWHLLRSLLRAL
                     RAAVLLSLIYAYVNSTLGLFPWTRGFAAEMLDLVLHPLVTIGRTALATIPDLAFLAVL
                     ALVTRLVLRLVRSFFVEIERGAVSFESFDPDWAMPTYRLVRLLIIAFAIVVAFPYIPG
                     SNTGAFKGVSLFVGVLVSLGSSSIIGNVIAGYTMTYRRAFRDGDVIQVGDILGQVTHT
                     RLLVTNVRTPKNIDVVIPNSLILSSHVMNYSALARDDGLILHTKVGIGYEVPWRQVEA
                     MLRAAAGRTEGLLTDPAPFVLQSALGDFAVTYELNTYCNQP"
     gene            complement(1612..2517)
                     /gene="pbpG"
                     /locus_tag="VD788_13065"
     CDS             complement(1612..2517)
                     /gene="pbpG"
                     /locus_tag="VD788_13065"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HXV77241.1"
                     /translation="MGCAAAVRSVVAIVAAAPLVAASGEIQLASVHAVVGDLERGEIL
                     YSKRDDIEVPIASVTKLMTAMVVLDSGRPLDEWVPIVRRGDSYGKNFHSRLRIGSQST
                     RGQLLRLALMSSENLASYVLAVHHPGGVDAFVEAMNAKARELGMSRTRFGDPTGLSTT
                     NRSTAADLFTLARAAYGYETIREYSTTYQYTARFRDPHYTLGFGNTNPLTASGRWEVG
                     MSKTGYLTEAGRCLVMVADIGGAPVGMVLLNSFGTRTPLGDAARVRRWLETGSSGRVA
                     GAALEYERRLAASYDRSVGLAERAP"
     gene            complement(2607..2981)
                     /locus_tag="VD788_13070"
     CDS             complement(2607..2981)
                     /locus_tag="VD788_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77242.1"
                     /translation="MGSNEHLRDRRRFVCSAIACIAGLPWLARCAGGPGGPDPAAVAE
                     QKQRYDPDLDCSDAKALWPAELATRTDNAYSDRSQNDPQYCFNCANFIAPPSGKGCGS
                     CRTVKGPVNPLGGCNIWVWKRA"
     gene            3105..5051
                     /locus_tag="VD788_13075"
     CDS             3105..5051
                     /locus_tag="VD788_13075"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF005558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HXV77243.1"
                     /translation="MQRNLSRKIIASHLLSGEMRPGSEIGLRIDQTLTQDATGTLVML
                     ELEAMGLDRVRTELSVQYVDHNTLQQDFKNADDHRFLMSCCRRLGIWFSPAGNGVSHP
                     VHQERFGVPGRTLLGSDSHTPAAGGLGMLAIGAGGLEVALAMAGEPFHVTMPEVWGVE
                     LRGTLPDWVSAKDVILEMLRRHGVAGAVGCILEYHGPGVATLSAMDRHVIANMGTELG
                     ATSTVFPSDERTLEFLRAQGRGAAWTALAADPGAAYDRSESIELDRLEPLIARPSSPG
                     NVVAVADEAGKEIAQAYIGSSANPGYRDFAVAARIVGDSRVDPRVSFDINPTSRQLLQ
                     NLIRDGHLSALVAAGARIHQAGCNGCIGMGQAPATDTISLRTVPRNFPGRSGTREDQV
                     YLCSPETAAASALTGRITDPRTLDMPYPRIGEPNRPVVSGETLVAPLDERAAAEVEPI
                     RGPNIRPLPELAPLGTELDAPVLLKLGDDVSTDEILPAGARVLPFRSNIAKIAEFAFD
                     VLDPEYHRRATETAGRGHVVVAGRNYGQGSSREHAALAPRSLGLELVIAKSFARIHRQ
                     NLINFGVLPCVFVDESDYDIVERDDRVRIADLPQVLRRDELVAEIGSKRRRVGLRHGL
                     SARQIEVLAAGGLIAWIKRKLDRS"
     gene            5048..>5260
                     /locus_tag="VD788_13080"
     CDS             5048..>5260
                     /locus_tag="VD788_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV77244.1"
                     /translation="MTRVVIVGGGFGGAYCARALERVPERDRLEVVLIDRKNYLLFYP
                     LLVEAGIGTLEPRHVGTPLRSFLRRAE"
BASE COUNT          876 a         1827 c         1801 g          756 t
ORIGIN      
        1 gggctggttg cagtaagtgt tcagttcgta cgtcaccgcg aagtcgccga gcgcggactg
       61 caggacgaag ggcgccgggt cggtcagcag cccctcggtg cgtcccgccg ccgcccgcag
      121 catcgcctcg acctgacgcc acggcacctc gtagccgatg ccgaccttcg tgtggagaat
      181 cagcccgtca tcgcgagcca atgcactgta gttcatcacg tgactcgaca ggatcaagga
      241 gttcgggatc acgacgtcga tgttcttcgg tgtacggacg ttggtgacga gcagccgcgt
      301 gtgcgtcacc tgtccgagga tgtcacccac ctggatgacg tcgccgtcgc ggaaggcgcg
      361 ccggtaggtc atcgtgtatc cggcgatgac gttcccgatg atcgacgacg acccgagtga
      421 caccaggacc ccgacgaaca gcgacacgcc cttgaacgcg cccgtgttcg agccgggaat
      481 gtaggggaac gcgacgacga tggcgaaggc gatgatcagc agccgcacca ggcggtaggt
      541 cggcatcgcc caatcgggat cgaagctctc gaagctgacc gcgccgcgct cgatctcgac
      601 gaagaacgac cgaacgaggc gcagcaccag ccgcgtcacc agcgccagga cggcgaggaa
      661 cgcgagatcc ggaatggtcg ccagcgccgt ccggccgatc gtgaccagcg ggtggagcac
      721 caggtcgagc atctccgcgg cgaatccgcg ggtccacggg aacaggccga gcgtcgagtt
      781 cacgtaggcg tagatcagcg acagcagcac ggcggcgcgc agcgctcgaa gcaggctccg
      841 cagcaggtgc caggttcgct cggcgctgag cagccggaac gactggatgc ggaggcgctc
      901 gacacccgcc tcgagccgac ggccgatcag gcgatcgaga cggcggaacg tccagccgac
      961 gaggatccac aggacgacca acaacacggt cgccaccacg acccagatcc cggagcgcag
     1021 cagtcgccgc ggcgaacgca gctcgcggta ttccgcgatc gccactcgga tgctgcgcag
     1081 gtggagctgt gccagctcgt gctggctcag cgcttcgagc gccgcgtccg attcgctggc
     1141 gacgacgatc gtcagcgacc cgtccccgac ggtgatgcgc ggaccgaagt ccgaggcctc
     1201 gacgcgcaac gcgccggggt cgatcgagcg gtcgcggccc accgtgatga ttcggtggcg
     1261 cacgtcggcg gcccgctcgg tggcgggata gccggacgta ccgcgcaggc gaaacagctc
     1321 gacgccgtcg aggacgaccg gcgcggtctc gggttggctc tcttgcccgt gcgccgccgc
     1381 gagcggcaac agcaacgccg ccgccagcag ggtcgtgcgc gtggcccgcg ccagacgaag
     1441 tcgagggaag gtggacgagg cgatgccgtt catggaaacg aacctcgctg tcgcccgacg
     1501 cggagcgacg gaggaggcgg acctcgccga gtcctccgtc ggaccggtcg gcgcgagccg
     1561 gcgggacgag ccgcggtccg gaccgcgcct acgatagcac cacggtcaac gctacggcgc
     1621 gcgctccgcc aacccgaccg atcggtcgta gctcgcggcc aaccggcgct cgtactccaa
     1681 cgcggccccg gcgacacgcc cgctcgaccc ggtttccaac cagcggcgga cgcgggcggc
     1741 gtcacccagc ggtgtgcgcg ttccgaacga gttgagcagc accatgccca ccggcgctcc
     1801 gccgatgtcg gccaccatca ccaggcaacg gccggcctcg gtcaaatagc cggtcttgct
     1861 catgccgact tcccagcgcc cgctcgcggt cagcggattg gtgttgccga agccgagcgt
     1921 gtagtgcggg tcgcgaaaac gcgccgtgta ctggtaggtc gtcgagtact cgcggatcgt
     1981 ctcgtaaccg taggccgccc gcgcgagcgt gaacaggtcg gcggcggtcg aacgattcgt
     2041 cgtcgacagg ccggtcgggt cgccgaaacg ggtccgtgac atgcccagct cgcgcgcttt
     2101 cgcgttcatc gcctcgacga aggcgtcgac gccccccggg tggtgcaccg ccagcacgta
     2161 ggaggcgagg ttctccgacg acatcagcgc caatcgcaac agttgcccgc gcgtcgactg
     2221 ggaaccgatc cgcaggcgcg agtggaagtt cttgccgtag gaatctcccc gacggacgat
     2281 cggaacccac tcgtcgagcg gccggcccga gtcgagcacg accatcgccg tcatcagctt
     2341 ggtcaccgac gcgatcggaa cctcgatgtc gtcccgcttg gagtagagaa tctcgccccg
     2401 ctcgagatcg ccgaccaccg cgtgaaccga agccagttgg atctcgccgc ttgccgcgac
     2461 cagcggagcg gccgcgacga tcgcgacgac cgatcggact gcggccgcac accccattcg
     2521 agcctccccc tgcgacagga ttcgtcacag cgtccgtgtg gtcgaacccg gcaagtatag
     2581 ggcccgacga cgatcggtgc acgggctcaa gcgcgcttcc agacccagat gttgcaacca
     2641 ccgagcgggt tgaccggtcc tttgaccgtc cggcagctgc cgcaaccctt gcccgacggc
     2701 ggcgcgatga agttggcaca gttgaagcag tactgcgggt cgttctgcga gcggtcggag
     2761 tatgcgttgt cggtgcgcgt cgcgagctcg gccggccaca gcgccttcgc atcggagcag
     2821 tcgagatcgg ggtcgtaccg ctgcttctgc tcggcgaccg ccgccgggtc ggggccgcct
     2881 ggccccccgg cgcagcgggc caaccacggg aggcccgcga tgcacgcgat cgcgctgcaa
     2941 acgaagcgcc tgcgatcgcg caggtgttcg ttcgacccca tcttgcgtcg tccgtggcga
     3001 ggcgaaacgc cgcgcgcgct cgacccgcgc gcaaccgaga ataccaaacc gacggctcgt
     3061 gcgataatga ccgtcgcggg cgccagcccg gtcctcggga cccgatgcaa cgaaacctgt
     3121 cgcggaagat catcgcctcg cacctgctct cgggcgagat gcggcccggc agcgagatcg
     3181 gcctgcgcat cgatcagact ctaacccagg acgcgacggg cacgctggtg atgctggaac
     3241 tcgaggcgat ggggctcgac cgcgttcgca ccgagctgtc cgttcagtac gtcgaccaca
     3301 acacgctgca gcaggatttc aagaacgcgg acgaccatcg cttcctgatg agctgctgcc
     3361 ggcgcctggg catctggttt tctccggcgg gcaacggcgt gagccacccc gtgcaccagg
     3421 agcgattcgg cgtgccgggc cgcacgctgc tcggctcgga cagccacacg cccgcggccg
     3481 gcgggctggg gatgctggcc atcggcgcgg gcggcctcga ggtcgcgctg gcgatggccg
     3541 gtgagccgtt ccacgtgacg atgccggagg tctggggcgt cgagcttcgc gggaccctac
     3601 cggattgggt cagcgccaag gacgtgatcc tcgagatgct gcgccggcac ggcgtcgccg
     3661 gcgcggtcgg ctgcatcctc gagtaccacg ggccgggcgt cgcgacgctg agcgcgatgg
     3721 accgccacgt gatcgccaac atggggaccg agctgggtgc gacgagcacc gtcttcccgt
     3781 ccgacgaacg aaccctcgag ttcctgcgcg cgcagggacg cggggcggcg tggaccgcgc
     3841 tcgcggcgga tcccggggcc gcgtacgatc gcagcgaatc gatcgagctc gaccggttgg
     3901 aaccgctgat cgcgcggcct tcgagcccgg gcaacgtcgt ggccgtggcc gacgaagccg
     3961 gcaaggagat cgctcaggcc tacatcggct cgtcggccaa tcccggctac cgcgacttcg
     4021 ccgtcgccgc gcggatcgtc ggcgactccc gcgtcgatcc tcgcgtctcg ttcgacatca
     4081 acccgacctc gcgccagctg ctgcagaacc tgattcgcga tggtcacctg tcggcgctgg
     4141 tcgccgccgg agcgcgaatc caccaggccg gctgcaacgg ctgtatcggc atgggccagg
     4201 ccccggcgac cgacacgatc agcctgcgaa cggtgccgcg caacttcccc gggcgctcag
     4261 gcacgcgcga ggaccaggtc tacctgtgca gccccgaaac cgcggctgca tcggccttga
     4321 ccgggcgaat caccgatccg cgcacgctcg acatgccgta tccgcgcatc ggcgagccga
     4381 accggcccgt cgtcagcggg gaaacgctcg tcgctccgct cgatgagcgc gccgccgccg
     4441 aagtggagcc gatcagggga ccgaacatcc gcccgctccc cgagctcgcg ccgctcggca
     4501 ccgagctcga tgctccggtg ctgctcaagc tcggcgacga cgtctcgacc gacgagattc
     4561 tgccggcggg cgcgcgtgta ctgccgtttc gcagcaatat cgcgaagatc gccgagttcg
     4621 ccttcgacgt gctcgacccg gaataccacc ggcgtgcgac cgagacggcc ggtcgcggtc
     4681 acgtcgtcgt cgccggacgc aactacggac aggggtcgag ccgcgagcac gcggcgctcg
     4741 ccccgcgctc gctcgggctc gaactggtca tcgccaagtc gttcgcgcgc atccaccggc
     4801 agaatctgat caacttcggc gtgctgccgt gcgtgttcgt cgacgagtcg gactacgaca
     4861 tcgtggagcg ggacgaccgc gtccggatcg ccgacctgcc gcaggtactg cgccgtgacg
     4921 agctggtcgc cgaaatcgga tcgaaacggc ggcgcgtcgg gctccgtcac ggcctgtccg
     4981 cgcgacagat cgaggtgctg gccgccgggg gtctgatcgc gtggatcaag cggaagctcg
     5041 accgatcgtg acccgtgtcg tcatcgtcgg cggaggcttc ggcggggctt actgcgcgcg
     5101 cgcgctcgag cgcgtcccag agcgggatcg gctcgaggtc gtattgatcg atcgcaagaa
     5161 ctacctgctg ttctatccgc tgctcgtcga ggccggcatc ggcacgctcg agccgcggca
     5221 cgtgggcaca ccgctccgat cgttcctgag acgcgcggag
//
LOCUS       DASPGN010000446         3089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1721423, whole genome shotgun sequence.
ACCESSION   DASPGN010000446 DASPGN010000000
VERSION     DASPGN010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721423"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VD788_13085"
     CDS             complement(<1..1163)
                     /locus_tag="VD788_13085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV77245.1"
                     /translation="MRVAYITAGAAGMYCGSCLHDNALAAAMIRAGHDVALIPVYTPI
                     RTDEEDVSVDRVFYGAINVFLEQKSALFRHTPWLVDRMLNSRSVLDWATRHTTSVDAK
                     ELGQLTLSVLEGEDGRQKKELDKLVVWLRDEVQPDVVNLPNSMLLGLAHRLREELGVP
                     VLCALQGEDIFLDELAPTFRERVSRLLRAKARDVDGFFAHSRYYVGAMAEMLDVARER
                     FHLVRLGIHLDGHGVVGPADEGRPFTVGYLARICPEKGLHVLVEAFQKLAGRVGAANV
                     RLRVAGYLGPRDRDYKRGILEQLAAWGLSESVDDLGEVDRERKLDFLRSIDVLSVPTT
                     YREPKGLYVLEAMANHVPVVQPRHGAFPELLDATGGGLLVEPNDTDALADALER"
     gene            complement(1198..2664)
                     /locus_tag="VD788_13090"
     CDS             complement(1198..2664)
                     /locus_tag="VD788_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HXV77246.1"
                     /translation="MIVSIIALYLVAVLAIGLASHRLFRGTGEDFFLATRTIGPFLLL
                     MSLFGTHMTAFSLLGASAEAYRHGIGVFALMASSSAIVAPTVFLFVGVRVWELGKRNG
                     FITQVQFFRDRFESDVLGLLLFFVVILLLIPYLLIGIKGGGLTLDSITGGTVPEWVGS
                     LAVCLVVLCYVTYGGLRGTAWANTFQTLLFMTLGAVAFIWITRELGGMGPILAQVGAD
                     RPDLLVRGEHIPPAKLLTYTCIPLSVGTFPHIFMHWLTARRAETFRVAIVAYPLCVAV
                     VWLPAVLLGIAGAAAIPGLEGPAASSVLVQLIDRYAPPALAGLLGAGVFAAIMSSLDS
                     QVLALGTIFTQDIVRHYRFHDRMSERQQVLVGRVFVVSILAATYSISLAAPSSIFKFG
                     IWSFTGFAALFPLVVAALFWRRSTGAAAGATALTVAALWLFFFARGWSVPNYSVAGSG
                     IMPVAVILVAASVVMVSVSLVTRPPSAATIERFFGDRR"
     gene            complement(2661..2957)
                     /locus_tag="VD788_13095"
     CDS             complement(2661..2957)
                     /locus_tag="VD788_13095"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HXV77247.1"
                     /translation="MSRDRSAPQPVGEASPPRPKRRGARPARVGVYLALIVLFALHND
                     LWLWDDPRFVLRLPVGLTYHVLYCVAAAVLMATVLRVTWPTPVVDRDGGARDDR"
BASE COUNT          595 a         1059 c         1003 g          432 t
ORIGIN      
        1 cgctcgaggg cgtcggccag cgcgtcggtg tcgttcggtt cgacgagcag cccgccgccg
       61 gtggcgtcga gcagctcggg gaacgccccg tgacgcggtt gtaccaccgg cacgtggttc
      121 gccatcgcct cgagcacgta caaccccttg ggctcgcgat aggtcgtcgg tacggacaag
      181 acgtcgatgc tccgcaggaa gtcgagcttt cgttcgcgat cgacctcacc gagatcgtcg
      241 accgattcgg acagacccca cgcggccagc tgctcgagga tcccgcgttt gtagtctcgg
      301 tcgcgcgggc cgaggtagcc tgccacccgc agccgcacgt tggccgcgcc gacgcggccg
      361 gccagcttct gaaacgcctc gaccagcacg tggagaccct tctccggaca gatccgcgcc
      421 aggtaaccga cggtgaacgg ccggccctcg tcggccggtc caacgacgcc gtgaccgtcg
      481 aggtggatgc cgagacggac caggtgaaac cgctcgcgcg ccacgtccag catctcggcc
      541 atcgcaccga cgtagtagcg gctgtgggca aagaatccgt cgacgtcccg cgccttggcc
      601 cgcagcagcc gcgacacgcg ctcacgaaac gtcggcgcga gctcgtcgag gaagatgtcc
      661 tcgccttgga gggcgcacag caccggcacg ccgagttcct cacgcaaccg gtgggccagc
      721 ccgagcagca tcgagttggg cagattgacg acatcgggct ggacctcgtc gcgcagccag
      781 acgacgagct tgtccagctc ttttttctgc cggccgtcct cgccttcgag caccgacaac
      841 gtcagctggc ccagctcctt ggcgtcgacc gaggtggtgt gccgcgtcgc ccagtcgagc
      901 acgctgcggc tgttcaacat ccgatcgacg agccacggcg tgtggcgaaa cagcgccgat
      961 ttctgttcca ggaagacgtt gatcgctccg tagaacacgc ggtcgacgct gacgtcctcc
     1021 tcgtcggtgc ggatcggcgt gtagaccggg atcaacgcga cgtcgtggcc ggcgcggatc
     1081 atcgccgcgg cgagcgcgtt gtcgtgcaga cagcttccgc agtacatgcc cgcggcaccg
     1141 gccgtgatat aggcgactct cattcgcttt cccccgctgt cggaccggtc gcccgcccta
     1201 gcgccgatcg ccgaagaaac gctcgatcgt cgcggcgctc ggcggacggg tgacgagtga
     1261 aaccgagacc atgacgaccg atgccgcgac caggatcacc gccaccggca tgattccgct
     1321 gcccgcgacg ctgtagttcg gcacgctcca cccgcgggcg aagaagaaca gccacagcgc
     1381 cgcgaccgtc agtgccgtcg cgccggcggc ggcccccgtc gaacggcgcc agaacaacgc
     1441 ggccaccacc agcgggaaca aggcggcgaa accggtgaac gaccagatgc cgaacttgaa
     1501 gatgctgctc ggcgccgcca acgagatcga gtaggtcgcg gccagtatcg aaacgacgaa
     1561 aacgcggccg accaggacct gctgccgctc gctcatccga tcgtgaaagc ggtagtgccg
     1621 tacgatgtcc tgggtgaaga tcgtccccag ggcgagcacc tgtgagtcga gcgacgacat
     1681 gatcgcggcg aagacccccg cgcccagcag ccccgcgagc gccggcggcg cgtatcggtc
     1741 gatcagctgc accaggacgg agctcgcggc cggtccttcg agaccgggga tggcggccgc
     1801 tcctgcgatg ccgagcagca ccgccggcag ccagacgacc gccacgcaca gcgggtaggc
     1861 cacgatcgcg acgcgaaagg tctcggcccg ccgggccgtc agccagtgca tgaagatgtg
     1921 cggaaaggtc ccgaccgaca gcgggatgca ggtgtaggtc agcaacttcg ccggtggaat
     1981 gtgctcgccg cgaacgagca gatccggtcg atcggctccg acctgggcca ggatcggacc
     2041 catcccgccg agctcccggg tgatccagat gaaagcgacg gctcccagcg tcatgaacag
     2101 caacgtctgg aacgtgttgg cccatgccgt tccgcgcagg ccaccgtagg tgacgtaaca
     2161 caatacgacg agacagacgg ccaggctgcc gacccactcc gggacggtgc cgccggtgat
     2221 gctgtccagt gtcaggccac cgcccttgat cccgatcagc aggtacggga tcagcaacag
     2281 gatgacgacg aagaacaaca gcagtccgag gacgtccgat tcgaagcgat cgcgaaagaa
     2341 ctgcacttgg gtgatgaacc cgtttcgctt accgagctcc cacactcgaa caccgacgaa
     2401 aaggaagacg gtcggagcca cgatcgccga agacgacgcc atcagcgcga agaccccgat
     2461 gccgtggcgg taagcctccg ccgatgcgcc caacagcgaa aaggccgtca tgtgcgtacc
     2521 gaacagcgac atcaacagca gaaacggccc gatcgtgcgg gtggcgagaa aaaagtcctc
     2581 gcccgtcccg cgaaacagcc gatggctggc cagcccgatc gcgagcaccg ctaccaggta
     2641 gagcgcgatg atcgagacga tcatcgatcg tcccgggcgc cgccgtcgcg gtcgacgacc
     2701 ggcgtcggcc aggtgacccg cagcacggtc gccatcaaaa ccgccgccgc gacgcagtac
     2761 agcacgtggt aggtcaagcc gaccggcagg cgcagtacga aacgcgggtc gtcccacagc
     2821 cacaggtcgt tgtgcagcgc gaacagcacg atcagcgcca ggtagacccc gacccgggcc
     2881 ggccgggcgc cgcgtcgctt cggcctcggc ggtgatgctt ccccgaccgg ttgtggcgcg
     2941 ctccgatcgc ggctcacatt cgatactgga acccgaagta aaccgccgtc gggttggccg
     3001 ggatgaccga cgtcgagccg aagccgcgca gctcgtattc ctcgtcggtc aggttcttga
     3061 aattcacgct gacccgcatg ctgggcagg
//
LOCUS       DASPGN010000447         3216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_784618, whole genome shotgun sequence.
ACCESSION   DASPGN010000447 DASPGN010000000
VERSION     DASPGN010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784618"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2044
                     /locus_tag="VD788_13100"
     CDS             <1..2044
                     /locus_tag="VD788_13100"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF006515.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HXV77248.1"
                     /translation="LSLIGAHETVLTQGFIARTPAGETCLLGRGGSDTSAALFAALLG
                     ADELEIWTDVPGMFTADPRRVPVARLIRRMGYREAQELAALGAKVLHPRCLAPLRARS
                     IPLSIHSTEDPAAEGTRVEATEEDHPTVTAVTCRNGVTLLSIASDEMWGEAGFMARVF
                     APFAEFGVSVDLVATSQYAITLTLDDPPGGADGPIVSRLVERLAGCGRVEVVHPCAVV
                     SIVGRRIRAALHELGPAMAVLKERPVHLVSDSAEDLNLSFVVDQADANTLVTRLHRQL
                     FGAQGGGPRFGPTWEMLRRDAGAHRAPPRWWAAERERLLALSRDGRARYVYHLPTIRA
                     RVRHLVERLASIDRVYYSMKANAHERVLETVAAAGCGIECVSAPELCHARRVLGPQVA
                     LLFTPNFCPLDEFELAFATGAEVVVDGPETLVAAPASFGGREIGLRIDPGRGLGHHEK
                     VRTAGSGVKFGHPIDDVERLRVAVERCGAKVVGLHAHVGSGIFDPVAWSTTGRALARL
                     VPAFPELRWIDLGGGLGVPENPGQPPLDLDEVERHLAGLAADLPQVELRLEPGRFLVS
                     EAGVLLLPVTQTRQKGGINFAGVSTGMNSLIRPALYGAWHPLHNLSRLDQPAREYWHV
                     VGPICETSDVLGRDRPLPATEPGDLLLAENAGAYGAVMASHYNLRPPAEESVIDDA"
     gene            2120..2410
                     /locus_tag="VD788_13105"
     CDS             2120..2410
                     /locus_tag="VD788_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77249.1"
                     /translation="MQVIHIIVLTGDDACWVARRIDYRIGEHGEPTAEAIESWIDALA
                     ERLERESRLDRAPRLVRPRPCENRLACWQQSEVDYRDALRQRGWASPSAHST"
     gene            complement(2353..>3216)
                     /locus_tag="VD788_13110"
     CDS             complement(2353..>3216)
                     /locus_tag="VD788_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77250.1"
                     /translation="WPAGFASHAAEAARALRDTADPATRQRHRERLRRELPLDREVLD
                     ELAGRTVDVMTVETSMIEAWELDWRPRPLLQSYAVGTARLDRLDADWFASTEAPERLL
                     VDLQGLDTRHPFMDAPRTWRVIFSRYEPLRRTQRWSVLALRDSPRAVSERPVDKLTVP
                     LNEAVRVPRTDAGHLELRVRLEPSLLGRLCGVAWKRPEVRLLVSTTRPAVPRRIVAAT
                     AGDPFPLVFPWIDSPEGLGRLFDAPALPAPYGLSFFTAGSWAWKHVEIDFVQVEWADG
                     DAQPRWRNASR"
BASE COUNT          462 a         1119 c         1169 g          466 t
ORIGIN      
        1 gctgtcgctg atcggtgcgc acgagaccgt gttgacacaa ggattcatcg cgcgcacgcc
       61 ggcgggtgag acctgcctgc tcggtcgtgg cggcagcgac acctcggcgg cgctgttcgc
      121 cgcactgctc ggcgccgacg agctcgagat ctggaccgac gtgcctggca tgttcaccgc
      181 ggatccgcga cgcgtgccgg tcgcgcgact gatccgcagg atgggatacc gcgaagccca
      241 ggagctggcc gcactcggag ccaaggtgct ccacccgcgc tgtctcgctc cgctgcgcgc
      301 gcgcagcatt ccgctcagca tccactcgac cgaagacccg gcggccgagg gtacgcgcgt
      361 cgaggccacc gaggaagatc atccgaccgt caccgcggtc acctgccgca acggcgtgac
      421 cctgctctcg atcgcgagtg acgagatgtg gggcgaggcg gggttcatgg cacgcgtgtt
      481 tgcgccgttc gccgagttcg gcgtgtcggt cgacctggtg gcgacctcgc agtacgccat
      541 cacgctgacg ctcgacgacc cgccgggcgg ggcggacggc ccgatcgtgt ctcgcctggt
      601 cgagcggctc gccggctgcg gacgcgtcga ggtcgtccat ccgtgtgcgg tggtttcgat
      661 cgtcggtcga cggatccgcg ccgcgctcca cgagctgggc ccggcgatgg cggtgctcaa
      721 ggagcgtccg gtccacctgg tcagcgactc ggcggaggat ctcaatctgt cgttcgtcgt
      781 cgatcaggcc gatgcgaaca cgctcgtcac gcggctgcac cgccaattgt tcggcgccca
      841 ggggggagga ccgcgcttcg gaccgacatg ggagatgctg cgacgcgacg ccggcgcgca
      901 tcgagctccg ccgcgctggt gggcggccga acgcgagcga ctgctcgcgc tgagtcgcga
      961 cggtcgggcc cgttacgtct accacctgcc gacgatccgc gcgcgcgtgc ggcatctggt
     1021 cgagcgtctg gcctcgatcg atcgtgtcta ctactcgatg aaggccaacg cgcacgagcg
     1081 ggtcctcgag acggtggccg cggccggttg tggtatcgag tgcgtctcgg ctccggagct
     1141 ctgtcacgcg cggcgcgtgc tcggaccgca ggtggcgctg ctgttcaccc ccaacttctg
     1201 cccgctcgac gagttcgagc tggcgttcgc gaccggcgcc gaggtggtcg tcgacggacc
     1261 ggagacgctg gtcgcggcgc ccgcatcgtt cggcggccgg gagatcgggc tgcggatcga
     1321 cccggggcgc gggctgggac accacgagaa ggtccgcacc gccggcagcg gcgtcaagtt
     1381 cggccacccg atcgacgacg tcgagcggct gcgcgtcgcg gtggagcgct gcggcgccaa
     1441 ggtggtcggc ctgcacgcgc acgtcggcag cgggatcttc gacccggtcg cgtggagcac
     1501 gaccggccgc gcgctcgcgc ggttggtccc ggccttcccc gagctgcgct ggatcgacct
     1561 cggtggcggc ctcggcgtac cggagaaccc ggggcaacca ccactcgacc tcgacgaggt
     1621 cgaacgtcac ctcgccgggc tcgcggccga tctgccgcag gtcgagttgc ggctcgaacc
     1681 gggccgattc ctcgtgagcg aggccggcgt cctgctgctt ccggtgaccc agacacgcca
     1741 gaagggcggc atcaacttcg ccggcgtgtc gaccgggatg aactcgctga tccgtccggc
     1801 gctgtacggg gcctggcacc cgttgcacaa cctcagccga ctggaccagc cggccaggga
     1861 gtactggcac gtcgtcggcc cgatctgcga gacgagcgac gtgctcggcc gcgaccggcc
     1921 gctaccggcg accgaaccgg gtgatctgct gttggccgag aacgccggtg cctacggcgc
     1981 ggtgatggcg tcgcactaca acctgcgccc gcccgccgag gaatcggtga tcgacgacgc
     2041 ctgacgggac gccggccggc ggcttgaccg ggcgcgaggc cgaccctagg tttcgtgcga
     2101 ggggtcgccg agggcgacga tgcaagtgat ccacatcatc gtcttgacgg gtgacgacgc
     2161 gtgttgggtc gcccgccgga tcgactaccg catcggcgag cacggcgagc cgacggcgga
     2221 ggcgatcgag tcgtggatcg acgcgttggc cgaacgtctc gagcgagaat cgcggctcga
     2281 ccgcgcgccg cggctcgtgc ggccgcgacc gtgcgagaat cggctggcgt gctggcagca
     2341 gagcgaggtc gactaccgcg acgcgttgcg ccagcggggc tgggcgtcgc cgtcggccca
     2401 ctcgacctga acgaagtcga tctcgacgtg cttccaggcc cagctgcccg cggtgaagaa
     2461 cgacaagccg tagggcgcgg gtagcgccgg ggcgtcgaac agccggccca acccctcggg
     2521 cgagtcgatc caggggaaga cgagcgggaa cgggtcaccg gccgtggcgg cgacgatccg
     2581 gcgcggtacc gccggccgtg tcgtgctgac caacagacgc acctcgggac gcttccacgc
     2641 cacgccgcaa agccgtccga gcaacgaggg ctcgagccgc acgcgcagct cgaggtgccc
     2701 ggcatcggtg cgcggtacgc gcaccgcctc gttgagcggg acggtgagct tgtcgaccgg
     2761 tcgctccgac accgcgcgag gcgagtcgcg cagcgccagc accgaccagc gctgcgtgcg
     2821 ccgcagcggt tcgtagcggc tgaagatcac ccgccaggtt cgcggggcgt ccatgaacgg
     2881 atgacgggtg tcgagcccct gcagatcgac gagcagccgt tcgggcgcct cggtcgatgc
     2941 gaaccagtcc gcatccagcc ggtccagtcg agccgtgccg acggcgtagc tttgcagcag
     3001 cgggcgcggg cgccagtcga gctcccacgc ctcgatcatc gacgtctcga ccgtcatcac
     3061 gtcgaccgtg cgccccgcca attcgtcgag cacctcccga tcgagcggca gctctcggcg
     3121 taaccgctcg cgatgacgct ggcgtgtggc cgggtccgcc gtgtcgcgaa gcgcgcgcgc
     3181 ggcctcggcg gcgtggctcg cgaatcccgc cggcca
//
LOCUS       DASPGN010000448         4785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_433941, whole genome shotgun sequence.
ACCESSION   DASPGN010000448 DASPGN010000000
VERSION     DASPGN010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433941"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..478)
                     /locus_tag="VD788_13115"
     CDS             complement(86..478)
                     /locus_tag="VD788_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77251.1"
                     /translation="MRFLLTFHGEMRWLVVLAAVVVALRSAYGWARGRSFGKGDRVAM
                     AVLTTLIDVNLLLGLVLLFRMPGGFPSYRLEHAVTMVIALLVAHSWMAWKRSEDSSLK
                     FRNNLLVALAVLVLIVLGVLRLRGAWVY"
     gene            614..1084
                     /locus_tag="VD788_13120"
     CDS             614..1084
                     /locus_tag="VD788_13120"
                     /inference="COORDINATES: protein motif:HMM:NF026066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXV77252.1"
                     /translation="MDKQEVVRTLEEMAEILELTEANPFQILAYRNGAQNLDDWAGDL
                     DRAVHDRTLTELPGIGKGLAAIITDLMLHGRSAECERLRGLVPRTLRELLNVPRLGPK
                     RIRKLHRELGVESLASLEAAAKQGRIRALAGFGPKSEEQIIRGIERARRYGRVP"
     gene            1074..1808
                     /locus_tag="VD788_13125"
     CDS             1074..1808
                     /locus_tag="VD788_13125"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXV77253.1"
                     /translation="MSRDSARGAIHAGTSGYSYKAWRGTVYPDELPQERFLRAYAERF
                     TTVEINNTFYRFPTDSVLKQWAAETPEGFTFAIKANQRITHRHRLKNADEVTRSFVER
                     CAVLERRLGPLLFQCPPVFRRDDERLAGFLRSLPAGARYAIEFRNKSWFEPPVAELLR
                     QAGVAFVQSDDDKLETPRFVTSSFCYVRLRRERYDDRRLADWRRWLDARRDDGLDVFV
                     YLKHDESGASPTIVLRSLGADEARSG"
     gene            1805..2557
                     /gene="bla"
                     /locus_tag="VD788_13130"
     CDS             1805..2557
                     /gene="bla"
                     /locus_tag="VD788_13130"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B1 metallo-beta-lactamase"
                     /protein_id="HXV77254.1"
                     /translation="MSGRVPCVVAVVVALALTCATAEDRRRRSLAEDLRVDRIAPGVW
                     RHESVQRQGELGPVAANGLIVVGRSAAALVDTPWTDGQTALLFDWVRASFDVEIEHVV
                     VTHSHADCSGGLAEAHRRGARSYGHRATAEIARAGRAPEPGVTFADEHRIDLDGTGLE
                     LRYFGAGHTRDNIVVWISPAKVLFGGCLVKPEGAGAGYIGEADLAAWPDTVRAVLAAF
                     PEARVVVPGHGEPGTLDYLRYTIRFTTELGAR"
     gene            2602..3630
                     /locus_tag="VD788_13135"
     CDS             2602..3630
                     /locus_tag="VD788_13135"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV77255.1"
                     /translation="MADNDRRSPRRRFLAQSMRCAGWLIGGLSLAPAAARCAFAAVAR
                     DQVVAEEPFARVERVADGVWAVVSTPLKDGGRQFATTSNGGIIAGREAVVIVEGYYSE
                     DGAAWVARQARELTGREPTHAVVTHYHADHTRGLDGLRAGRDEGAGALSLLATATTRE
                     LLVETEEPVVPDNEISAEGAEIDLGGRRLRLVPRLGHTRSDVTVHLDEPQVVWCGDLV
                     WNGMFPNYVDAVPSLLTEHCAALLGRRGTTYVPGHGDVADAGALDDYLALIRDLEAAG
                     RRAVEQGLPPAEAAAAYRIPQSIERWTMFSPTYVERAFEAWQRELGGTDASAGSRVSP
                     PSGSRRCR"
     gene            complement(3596..>4785)
                     /locus_tag="VD788_13140"
     CDS             complement(3596..>4785)
                     /locus_tag="VD788_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXV77256.1"
                     /translation="RRRRKENSMTIRIDSMVGAAALLATLGATGTAAERATKTFPADS
                     AQRLTLKLEAGGTVRVVGDGGSEITVSYDISATSAGDCTVDAAADADGVTVISRFRSA
                     GERRGSNIEIEVHVPRYFDVELDSIGGGLELEGVEGEFRGQTMGGALTLRDVRGEARL
                     ETMGGAITLVDSELDGKLSTMGGEVLFDNVIGDVTGSSMGGNVRYRNVRRRDGSVAKL
                     GSLADVDVSEETVQISTMGGQISVERAAEGAALHTMGGDIRVEDAQRFVRAKTMGGDI
                     VVEAVDGWVRATTMGGDIEVTLVGDGGDVDLESMSGDLLLVVPPGFSMELDIEIAFTR
                     GSSEDYRITSEYDLEQTVSPGWDHAHGSPRKYIRAGGRTGGGDKRVKLRTVNGNVEIR
                     TGG"
BASE COUNT          730 a         1680 c         1626 g          749 t
ORIGIN      
        1 gttcgcgtgg gagcgtgccg ccggtgctcg aaccatgcgc cgtggtcgca cagcagctgg
       61 cggatcgctt cgtgcaggtc gccggtcaat agacccacgc tccgcgcagg cgaagcacgc
      121 cgagcacgat cagcacgagg acggcgagcg cgaccagcag gttgttgcgg aacttgagcg
      181 acgagtcctc ggaccgcttc catgccatcc acgagtgagc gacgagcaac gcgatcacca
      241 tcgtgacggc gtgctcgagc cgataggacg ggaagccgcc cggcatccgg aacagcagca
      301 ccagaccgag gagcagattc acgtcgatca acgtggtcaa taccgccatc gcgacgcgat
      361 cgcccttgcc gaacgaccga ccgcgcgccc agccgtacgc cgaacgcagc gcgacgacta
      421 ccgccgccag cacgaccagc caccgcatct ctccgtggaa cgtcagcagg aatcgcattc
      481 tcgacactcc tcgtcgcgag ccctcgcgtc ggatcgggta gcatacccga tcggcgatcg
      541 gttgaccccc cgcccctctc gcggttatct tgttcgcgac tccgacttcg ctcgaaaaaa
      601 ccggggtgcg cgtatggaca agcaggaggt agtgcgtacg ctcgaagaga tggcggagat
      661 cctcgagctg accgaggcca acccgtttca gatcctggcc taccgcaacg gtgcgcagaa
      721 tctcgacgat tgggccggtg acctcgaccg ggcggtgcac gaccgcacgc tcaccgagct
      781 gccggggatc ggcaaggggc tcgccgcgat catcaccgat ctgatgctcc acggtcgctc
      841 cgccgaatgc gaacgattgc gcggattggt gcctcggacc ctacgggagc tgctcaacgt
      901 cccgcggttg ggtcccaagc gcatccgcaa gctccaccgc gagctcggcg tcgagagcct
      961 cgcctcgctc gaggccgcgg ccaagcaggg tcgcatccgc gcgctcgccg gtttcggacc
     1021 gaagagcgag gagcagatca tccgcggaat cgagcgggcg cgtcgttacg gccgtgtccc
     1081 gtgattccgc gcgcggcgcg atccacgccg gaacctcggg ctactcgtac aaggcatggc
     1141 gcggtacggt ctatcccgac gagctgccgc aggagcgctt cctgcgggcc tacgccgaac
     1201 ggttcaccac cgtcgagatc aacaacacct tctatcgatt cccgaccgac agcgtgttga
     1261 agcagtgggc cgccgaaaca cccgagggct tcaccttcgc gatcaaggcc aaccaacgga
     1321 tcacgcaccg ccatcggttg aagaatgcgg acgaggtgac gcgatcgttc gtcgagcgct
     1381 gtgcggtgct cgagcgacgg ctcggtccgc tgctgttcca atgtccaccg gtcttccgtc
     1441 gggacgacga gcgtctggcc gggttcctgc ggtcgctgcc ggccggcgca cggtacgcga
     1501 tcgagttccg caacaagagt tggttcgaac caccggtcgc cgagctgctc cggcaggccg
     1561 gggtggcgtt cgtccagtcc gacgacgaca agctcgagac gccgcgtttc gtcacgagct
     1621 cgttctgcta cgtccggctc cgccgcgagc gttacgacga ccggcggctg gcggactggc
     1681 gccgctggct cgacgcacgg cgcgacgacg gtctcgacgt cttcgtctac ctcaagcacg
     1741 acgagtcggg ggcgtcgccg acgatcgtgt tgcgctcgct cggcgccgac gaggcgcgga
     1801 gcggatgagc ggacgggtcc cgtgcgtggt cgcggtggtc gtcgcgctcg cgctgacctg
     1861 cgccacggcc gaggaccgac gccggcggtc gctcgccgag gatctgcgcg tcgaccgcat
     1921 cgcaccgggg gtgtggcggc acgagtcggt ccaacgccag ggcgagctcg gaccggttgc
     1981 cgccaacggc ctgatcgtcg tcggccgctc ggccgcggca ctggtcgaca cgccgtggac
     2041 cgacggccag accgccctcc tgttcgattg ggtgcgcgcg agcttcgacg tcgagatcga
     2101 acacgtggtc gtgacgcact cgcacgcgga ctgcagcggc gggttggccg aggctcatcg
     2161 gcgcggcgcg cgatcgtacg gccatcgggc gacggccgag atcgcccggg cggggcgagc
     2221 ccccgagccc ggcgtcacct tcgccgacga acaccggatc gatctcgacg gaacgggact
     2281 cgagctgcgc tatttcggcg cgggacacac gcgcgacaat atcgtcgtct ggatctcgcc
     2341 ggcgaaggtg ttgttcggcg gatgcctggt caagcccgag ggcgccggtg ccggctacat
     2401 cggcgaagcc gacctggcgg cctggcccga cacggttcgc gcggtcctcg ccgccttccc
     2461 cgaggcgcgg gtcgtcgtgc ccggccacgg cgagccgggt accctcgact acctgcgcta
     2521 cacgatccgt ttcacgaccg agctcggcgc gcgctagcgt ttcccgcggc tcgatgacct
     2581 atactgccgg cgatggtcgg ggtggccgac aacgatcgcc ggagcccgcg ccggcggttt
     2641 ctcgcgcagt cgatgcgttg cgccggttgg ctgatcggcg gcttgtcgtt ggcaccggcg
     2701 gcggcgagat gcgcgttcgc cgcggtggcg cgagaccagg tcgtggccga ggagccgttc
     2761 gcacgtgtcg agcgtgtggc ggacggcgtg tgggccgtgg tctcgacgcc gctcaaggac
     2821 ggtggacggc agttcgccac gacgtcgaac ggagggatca tcgccggtcg cgaggcggtg
     2881 gtcatcgtcg aaggctacta ctcggaggac ggggcagcgt gggtcgcgcg gcaggcgcgc
     2941 gagctgaccg ggcgcgagcc gacccatgcg gtggttacgc actaccacgc cgatcacacc
     3001 cgcgggctgg atgggctgcg tgccgggcgt gacgaggggg ccggtgcgtt gagcctgctc
     3061 gcgaccgcga cgacgcgcga gctgctcgtc gagaccgaag agccggtcgt gccggacaac
     3121 gagatctccg ccgagggcgc ggagatcgac ctcggcggcc ggcgcttgcg gctcgtgccg
     3181 cgcctcggtc acacgcgaag cgacgtcacc gtccacctcg acgagccgca ggtggtgtgg
     3241 tgcggcgatc tggtctggaa cgggatgttc ccgaactacg tcgacgccgt cccgtcgctt
     3301 ctcaccgagc actgcgccgc actgctcggg cggcgaggca cgacctacgt cccggggcac
     3361 ggtgacgtcg ccgacgccgg agcgctcgac gactacctcg cgctgattcg cgacctcgag
     3421 gcggccggac gacgcgccgt cgagcagggg ctaccgccgg ccgaggccgc cgcggcgtac
     3481 cgcatcccgc aatcgatcga gcgctggacg atgttctcgc cgacctacgt cgagcgtgcg
     3541 ttcgaagcct ggcaacgcga gctcggggga acggacgcca gtgccggtag ccgcgtcagc
     3601 cccccgtccg gatctcgacg ttgccgttga cggtgcgcag cttgacccgc ttgtccccgc
     3661 cgccggtgcg accaccggcc cggatgtact tccgcggcga gccgtgggcg tggtcccagc
     3721 ccggggaaac cgtttgttcg agatcgtatt cggaggtgat gcggtaatcc tccgagctgc
     3781 cgcgggtgaa cgcgatctcg atgtccagct ccatcgagaa gcccggcggc acgacgagca
     3841 gcaggtcgcc cgacatcgat tcgagatcga cgtctccccc gtcgccgacg agcgtcactt
     3901 cgatgtcgcc gcccatcgtc gtcgcccgga cccagccgtc gaccgcttcg accacgatgt
     3961 cgccgcccat cgtcttggcg cggacgaacc gctgcgcgtc ctcgacgcgg atgtcgccgc
     4021 ccatggtgtg gagtgccgcg ccttcggctg ctcgctcgac gctgatctgc ccacccatcg
     4081 tcgagatctg gacggtttct tcgctgacgt cgacgtccgc caggctaccg agcttcgcca
     4141 ccgaaccgtc ccggcggcgc acgttcctgt agcgcacgtt gccacccatc gacgacccgg
     4201 tgacgtcgcc gatcacgttg tcgaacaaca cctcgccgcc catcgtgctc agcttgccgt
     4261 ccagctcgga gtcgaccagc gtaatcgccc cacccatcgt ttcgaggcgc gcctcgccgc
     4321 gcacgtcgcg cagggtcagc gccccgccca tcgtctggcc gcggaactcg ccttcgacac
     4381 cttccagctc gagtccgccg ccgatcgaat cgagctcgac gtcgaagtaa cgtggcacgt
     4441 ggacctcgat ctcgatgttc gagccccggc gttcgccggc cgagcggaag cgactgatga
     4501 cggtgacgcc gtccgcgtct gccgccgcat cgaccgtgca gtccccggcg ctggttgccg
     4561 agatgtcgta gctgaccgtg atctccgagc cgccgtcgcc gacgacacgt acggtccccc
     4621 cggcctcgag cttcagcgtc aaccgttgag cggaatccgc ggggaaggtc ttggtcgcgc
     4681 gctcggccgc cgtgccggtc gccccgaggg tggccagcag cgccgcagcg ccgaccatac
     4741 tgtcgatccg gatcgtcatc gagttctcct ttctccgtct tcgtg
//
LOCUS       DASPGN010000449         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1253292, whole genome shotgun sequence.
ACCESSION   DASPGN010000449 DASPGN010000000
VERSION     DASPGN010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253292"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3636
                     /locus_tag="VD788_13145"
     CDS             <1..>3636
                     /locus_tag="VD788_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77257.1"
                     /translation="TPTDFSDDELNIEAGIGFYAMLDNLSLVSLKDNNATPTQANDDK
                     SYLGLELSGLSAALIGIEGLTISVTDATVKVNQASDTDGILATNPAKLDWVAFFANAV
                     GLVPASVPNLAPTDDLLVAGNLLLEVGDFVQIRGGFAFTRSSDLMVTVSGAGMTTTMK
                     SVSVVTIGMQDVNVFAGAGPYFVDSDMDGDYDDDDTPSADAVGLLLKDVTVGIALFKP
                     TNRADTSSYYAIRAEAESIELPGLDLGGSETFSLTASGYRVEVSGGKEGASPLAKPAI
                     NFAALPGGSFLIPTGPASSLEIDYSSPLLRVAIERATLVIDEYVYVSGGFAFTKLDAL
                     PVKLSDTAQTPRTMSAYAFGAGNVDLFVGSGPYFVDSDGDGDIDEFDERDDDAIGLAL
                     ENVNFGLILMRPTTPAFQSIKYLSLQATANFAGLVGLDVLKLQASGISVEYNSVSNPG
                     NPNDTIVVDFAESFQGTGGYALDTGNGELLFGYSDKLLRATVDDALLQIDEYVFIRGS
                     LAFEQGPVQTVQLSPTGSKMVTSLNIGAEGLNIFFGVNGPYWNDLDGDGVVDDPEELN
                     EDAIGLAITNADLALAILRPTVMADTTRYVALKASADHVGFVGVDVFQLEASSVVVEL
                     NLATGAGTTDVSPVVDFAATYPGEYAALFDVFDTDGVGGITEAELDAALLDDDHGVTE
                     ALTTAEQLVELLDAGGAPPTGVLTVEEVLSQLDSAFHGAAVAADADGDGKIDPVGYEI
                     ATGTSTFTYLTASQRQIHASADDVLINVAEFVYLKANFAIDLGSREFVTIRTGVPASI
                     GSLASGAVTLVNTALSDLSTQLDGLHTEIKAAVTSAINTIKTEIQNQVSSIVDDIVAQ
                     LQSTLENAIATVSGNIKAQLEAVTAGLSVDSLVDPLLASITDQVPEGPLRSMVAALVS
                     PIKALLAGYFKDTLEEALSGAVDRIAGSVGAALQAGLQSAANEIEAKILEVLNPQIAR
                     IQLKLDELIFRIDSALTPIFARLQSITGIDLGSNFSTIEDFEVEVSAIGISDATAFIG
                     IPPDGGFDWNQPLADQDAIGLFVENLNLALGIFQSPLSKQIPPFVAAKVNADAAGFIS
                     GGLDDVLTILAEGIEVQLNLGAPIVPGSGTLFPNATIDFVASFPAEPPAGVNPGKPAG
                     YAVQTGTTTEPIYIDFQGELIVASVEVATIGISEFVHITGSIAFEKGRVHTVQVTGGL
                     LTDAAEV"
BASE COUNT          648 a         1265 c         1159 g          564 t
ORIGIN      
        1 ccacgccgac ggacttctcg gacgacgagc tcaacatcga ggcgggcatt ggcttctacg
       61 ccatgctgga caacctgagc ctggtgagcc tgaaggacaa caacgccacg cccacgcagg
      121 cgaacgacga caagagctac ctcgggctcg agctcagcgg attgtcggct gcgctgatcg
      181 gcatcgaagg cctgaccatc agcgtcaccg acgccacggt gaaggtaaac caggcaagcg
      241 acaccgacgg cattctcgcc accaacccgg cgaagctgga ctgggtcgcg ttcttcgcca
      301 acgccgtggg cctcgtgccg gcgagcgtgc cgaatctcgc gcccacagac gatctgctcg
      361 tcgcgggcaa cctactgctc gaagtcggcg acttcgtcca gatccgcggc ggtttcgcct
      421 tcacccgttc ctccgacctg atggtgacgg tctcgggcgc gggcatgacg acgacgatga
      481 agagcgtgag cgtggtcacc atcggcatgc aggacgtgaa cgtctttgcc ggcgcgggcc
      541 cgtacttcgt cgacagcgac atggacggcg actacgacga cgacgacacg ccgagcgcgg
      601 acgcggtcgg cctgctgctg aaggacgtta cggtcggcat cgcgctgttc aagcccacca
      661 accgtgcgga cacgagcagc tactacgcca tccgcgccga ggccgaatca atcgagctgc
      721 ctggcctgga tctcggcggc tccgagacct tctcgctgac cgcgagcggc taccgcgtcg
      781 aggtaagcgg cggcaaggaa ggcgccagcc ccctcgccaa gccggccatc aatttcgcgg
      841 cgctgccggg cggcagcttc ctcatcccga ccgggccggc gagcagcctc gagatcgact
      901 acagctcgcc gctgctgcgc gtggccatcg agcgcgcgac cctcgtcatc gacgagtacg
      961 tgtacgtctc gggagggttc gcgttcacca agctcgatgc gctacccgtg aagctgagcg
     1021 acacggcgca gacgccgcgc acgatgtcgg cctacgcctt cggcgcgggc aacgtcgacc
     1081 tcttcgtcgg cagcgggccg tatttcgtgg atagcgacgg cgacggcgac atcgacgaat
     1141 tcgacgagcg tgacgacgac gcgatcggcc tcgcgctgga gaacgtgaat ttcggcctca
     1201 tcctgatgcg gccgacgacg ccggccttcc agtcgatcaa gtacctgtcg ctccaggcga
     1261 cggcgaattt cgcggggctg gtgggccttg acgtcctgaa gctccaggct tcgggcatca
     1321 gcgtggagta caactcggtc agcaacccgg gcaacccgaa cgacacgatc gtcgtcgact
     1381 ttgcggagag cttccagggc acgggcggat acgccctcga caccggaaac ggcgagctgc
     1441 ttttcggcta cagcgacaag ctgctgcgcg ccaccgtcga cgacgcgctg ctgcagatcg
     1501 acgagtacgt cttcatccgc ggctcgctcg ccttcgagca ggggccggtg cagacggtcc
     1561 agctgtcgcc caccggctcg aagatggtga cctcgctgaa catcggcgcc gagggcctga
     1621 acatcttctt cggcgtgaac gggccgtact ggaacgacct cgacggcgac ggcgtggtcg
     1681 acgatcccga ggagctgaac gaagacgcga ttggcctcgc catcaccaac gccgacctgg
     1741 cgctcgcgat cctgcggccg acggtcatgg cggacaccac gcgctacgtc gccctcaagg
     1801 ccagcgccga tcacgtcggc ttcgtgggtg tcgacgtgtt ccagctcgag gcttcgtcgg
     1861 tcgtcgtcga gctgaacctg gcgaccgggg cgggcacgac cgacgtgtcc ccggtggtcg
     1921 acttcgccgc cacctatccg ggcgaatatg cggcgctctt cgatgtcttc gatacggatg
     1981 gcgtgggcgg cattacggag gccgagctcg atgccgcgct ccttgacgac gaccacggcg
     2041 tcaccgaggc gctgacgacc gccgagcagc tggtcgagct gctcgacgcc ggcggcgcgc
     2101 cgccgacggg cgtgctcacc gtcgaagaag tcctctccca gctcgacagc gccttccatg
     2161 gcgccgccgt cgcagccgac gcggacggtg acggcaagat cgatcccgtc ggctacgaga
     2221 tcgccaccgg caccagcaca ttcacctacc tgaccgcatc gcagaggcag atccatgcct
     2281 ccgccgacga cgtcctcatc aacgtcgcgg agttcgtcta cctcaaggcg aatttcgcga
     2341 tcgacctcgg cagccgcgag ttcgtcacca tccgcaccgg cgtcccggcc agcatcggct
     2401 cgctggcgtc gggcgcggtg accctggtca acaccgccct gagcgatctc tcaacgcagc
     2461 tcgacggcct gcacacggag atcaaggccg cggtgacctc ggcgatcaac acgatcaaga
     2521 ccgagatcca gaaccaggtg agctcgatcg tcgacgacat cgtcgcccag ctgcagtcga
     2581 cgctcgagaa cgcgatcgcc acggtaagcg gcaacatcaa ggcgcagctc gaggcggtga
     2641 ccgcgggcct gtcggtcgac agcctggtcg atccgctgct cgcgtccatc accgaccagg
     2701 tgccggaagg cccgctgcgc agcatggtcg ccgcgctcgt cagcccgatc aaggcgctgc
     2761 tcgcaggcta tttcaaggac acgctcgagg aggcgctgtc cggcgcagtc gaccgcatcg
     2821 ccggctcggt cggtgccgcc ctgcaggcgg ggctgcagag cgccgccaat gagatcgagg
     2881 cgaagatcct cgaggtgctc aacccgcaga tcgcccgcat ccagctcaag ctcgacgagc
     2941 tgatcttccg tatcgacagc gcgcttacgc cgatcttcgc ccggctgcag agcatcaccg
     3001 gcatcgatct cggcagcaac ttctcgacca tcgaggactt cgaggtcgag gtcagcgcga
     3061 tcggcatcag cgacgcgacg gccttcatcg gcatccctcc cgacggcgga ttcgactgga
     3121 accagccgct cgccgaccag gatgcgatcg gcctgttcgt cgagaacctg aacctggcgc
     3181 tcggcatctt ccagtcgcct ctcagcaagc agatcccgcc gttcgtggcc gcgaaggtga
     3241 acgccgatgc cgcgggcttc atcagcggcg gcctggacga cgtgctgaca atcctcgccg
     3301 aaggcatcga agtgcagctc aacctcggcg ccccgatcgt gccgggctcg ggcacgctgt
     3361 tccccaacgc cacgatcgat ttcgtcgcga gcttcccggc ggagccgccg gccggcgtca
     3421 accccggcaa gcccgcgggc tacgcggtgc agaccggcac caccaccgag cccatctata
     3481 tcgacttcca gggcgagctg atcgtggcga gcgtcgaggt ggcgacgatc gggatttccg
     3541 agttcgtgca catcaccggc agcatcgcct tcgagaaggg ccgcgtgcac accgtccagg
     3601 tgacgggcgg cctgctgacc gacgcggcag aagtca
//
LOCUS       DASPGN010000450         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1253712, whole genome shotgun sequence.
ACCESSION   DASPGN010000450 DASPGN010000000
VERSION     DASPGN010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253712"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VD788_13150"
     CDS             <1..632
                     /locus_tag="VD788_13150"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXV77258.1"
                     /translation="WSALRRPWPSCDIRILAAMGRQREASIVPLTGAPDAVDASPPRP
                     LALSPVELPRERRPGWPTLAALALATGLAAIGIGAWSILAETRSEPAPAADAAVERSL
                     AVLADPRAERYALRGSVGRIALVVTASGDAVLALDGLGPPAAGSTYRAWLVPPGSATP
                     VGDAAFDATSRVVPLDRRVPRGTRVAVTLELSADALRPSRPLRLSVVRE"
     gene            complement(780..1790)
                     /locus_tag="VD788_13155"
     CDS             complement(780..1790)
                     /locus_tag="VD788_13155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXV77259.1"
                     /translation="MRTRMDDLGRDLSEALERAERESKRNRFLSDLGNSIEFEELLDR
                     VLDAALEVPGFDAAMVVLDDPGGSPTIATRGMTPEEAAHPPSSGAAGAQPAGPITVSY
                     RYGASDAADTHLIRGGLFQPLVSRDGNALGSLALFWRTPGYEPSHDRVSAIEQIAESC
                     IPAVENARRYREARQLAETDALTGFFNQRYFHETLRREALRAQRYDRRLALLILDLDD
                     FKAVNDRIGHLAGDAVLAQVAERLRNEIRSVDIGCRVGGDEFAVIMPESTTEDGGQLF
                     QRMHDSLVGMSVPGGQTVRMSAGIAELRHGETAAGLFERADSALYRAKDLGKDRASVA
                     KD"
     gene            2147..2521
                     /gene="rpsL"
                     /locus_tag="VD788_13160"
     CDS             2147..2521
                     /gene="rpsL"
                     /locus_tag="VD788_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HXV77260.1"
                     /translation="MPTVNQLVRKGRKAAKARVKTPALRGAPQKRGVCTRVYTTTPKK
                     PNSALRKVARVKLTNQIEVGAYIPGEGHNLQEHSVVLIRGGRVRDLPGFRYKVIRGGL
                     DTSGVSERRQGRSKYGAKKGGS"
     gene            2526..2996
                     /gene="rpsG"
                     /locus_tag="VD788_13165"
     CDS             2526..2996
                     /gene="rpsG"
                     /locus_tag="VD788_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXV77261.1"
                     /translation="MPRRAEIQPRTVEPDPVYGSVLVTQLVNRLMLDGKKSIAEAIVY
                     DALAQVAEKTGKPALESLEAAVKSVTPVLEVRSRRVGGANYQVPVEVPPRRGRTLALR
                     YLVTYARERREKGMQSKLAAELMDAINQQGGAFKRKDDMYRMAQANKAFAHYRW"
     gene            3013..>3522
                     /locus_tag="VD788_13170"
     CDS             3013..>3522
                     /locus_tag="VD788_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXV77262.1"
                     /translation="MASQVGLDRVRNIGIMAHIDAGKTTTTERILYYTGRTHKLGEVH
                     EGAATMDWMAQEQERGITITSAATTAFWRDHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VAVFDSVAGVQPQSETVWRQADRYGVPRIAFINKMDRTGADFDAAVQSMRDRLGANPV
                     PIQIPIGQED"
BASE COUNT          505 a         1288 c         1241 g          488 t
ORIGIN      
        1 gatggtcggc tcttcgacga ccctggccgt catgcgacat ccgtatcctc gccgccatgg
       61 gccggcagcg cgaagcgagc atcgtcccgc tcacgggcgc gcccgacgca gtcgacgcga
      121 gcccgccgcg ccccctcgcc ctgtcgcctg tcgagctccc gcgtgagcgg cgaccggggt
      181 ggccgacact cgctgcgctc gcgctcgcaa ccggcctcgc cgcgatcggg atcggcgcct
      241 ggtcgatcct cgccgagacg cggtcggagc ccgctccggc agcggacgcc gccgtggagc
      301 gctcgctcgc cgtgctcgcc gacccgcgcg cggagcgcta cgcgctccgc ggctcggtcg
      361 gtcggatcgc gctcgtggtg acggcctccg gcgacgccgt cctcgcgctc gacggcctcg
      421 gtccgccggc ggccgggagc acctaccgcg cgtggctcgt tcccccggga tccgcgacgc
      481 cggtcggcga cgccgccttc gacgcgacgt cccgcgtcgt cccgctcgac cggcgggttc
      541 cgcgcggaac ccgcgtcgcc gtcacgctcg agctctcggc cgacgccctc cggccgtctc
      601 gaccgctccg gctctccgtc gtccgcgagt agcccagccg gcactcgagc gagtcgcggg
      661 aggcgtctcc gaggcagttg cggccccgaa aggggacgcc ttcaggggtg tgcccgctcg
      721 gagggcgggg atccgagcgc gcgattccgc ttctgcggaa tcccgcttgc gaacaagcat
      781 cagtccttgg cgacgctcgc ccggtccttg ccgaggtcct tcgcgcggta gagggcagag
      841 tcggcgcgct cgaagaggcc cgccgccgtc tcgccgtggc ggagctcggc gatcccggcc
      901 gacatccgca ccgtctgccc gcccggcacg gacatcccga ccaggctgtc gtgcatccgc
      961 tggaacagct ggcctccgtc ctcggtcgtc gactccggca tgatcaccgc gaactcgtcg
     1021 ccgccgacgc ggcagccgat gtcgaccgag cggatctcgt tgcggagtcg ctccgcgacc
     1081 tgcgcgagga cggcatcgcc cgcaaggtgg ccgatccggt cgttgaccgc cttgaagtcg
     1141 tcgaggtcga ggatcaggag cgcgagccgg cggtcgtagc gctgcgcgcg gagggcctct
     1201 cggcggaggg tctcgtggaa gtaccgctgg ttgaagaagc cggtcagcgc gtcggtctcg
     1261 gcaagctggc gcgcttcccg gtagcggcgc gcgttctcga ccgccgggat gcacgactcc
     1321 gcgatctgct cgatcgccga tacccggtcg tgcgacggct cgtatcccgg cgtgcgccag
     1381 aagagcgcca ggctcccgag cgcgttcccg tcccggctga cgagcggctg gaacagcccg
     1441 ccgcggatga ggtgggtgtc ggccgcgtcg gacgcgccgt agcggtagct gacggtgatc
     1501 ggcccggccg gctgggcgcc ggcggctccc gacgacggcg gatgcgcggc ttcctccggc
     1561 gtcatgccgc gtgtggcgat cgtcgggctc ccccccgggt cgtcgagcac gaccatcgca
     1621 gcgtcgaagc cgggcacctc gagcgccgcg tcgagcacgc ggtcgagcag ctcctcgaac
     1681 tcgatcgagt tgccgaggtc gctcaggaac cggttgcgct tgctctcgcg ctcggcgcgc
     1741 tcgagcgcct cggagaggtc gcggccgagg tcgtccatgc gagtgcgcat gtcgtcgacc
     1801 gcccgggcga gacgatcctc gcggtgcgcg tcgggcctgt tccggagcgc gagcgtgacg
     1861 gcgagcgccc cgcccccgag aagggcggtt gccgcgacga cgagaatgat ggccgttccg
     1921 ctcgagatct ccggtccccc aacccttccc cgcacgcaat gtagtcggca acgcgccgga
     1981 acggaatccc cggcagcgca atgcccgtga attcgcatca cgctgcggtc gctccccgac
     2041 gcaggccgtc gctttcggct accatggccg ctcccgggcg gcgggacgtc gtgcctgccg
     2101 gccctttctc tgcgagaaaa gcttcgatcg aaacgacagg agtagcgtgc ccaccgtcaa
     2161 ccagctcgtc cgcaagggcc ggaaggccgc caaggcgagg gtcaagaccc cggcgctccg
     2221 cggcgccccc cagaagcggg gcgtctgcac gcgcgtctac acgacgaccc cgaagaagcc
     2281 gaactcggcg cttcgcaagg tcgcgcgcgt caagctgacg aaccagatcg aggtcggggc
     2341 gtacatccct ggcgaggggc acaacctcca ggagcactcg gtcgtcctga tccgcggcgg
     2401 ccgcgtccgg gacctgccgg gcttccgcta caaggtgatc cggggcgggc tcgacacctc
     2461 cggtgtctcg gagcgccgcc agggccgctc gaagtacggc gccaagaagg gcggcagcta
     2521 gacccatgcc gcgccgcgcc gagatccagc cgcgcacggt cgagccggac cccgtctacg
     2581 ggtcggtgct cgtcacgcag ctcgtcaacc gcctgatgct cgacggcaag aagtcgatcg
     2641 ccgaggcgat cgtctacgac gcgctcgcgc aggtcgccga gaagaccggc aagccggcgc
     2701 tcgagtcgct cgaggcggcg gtcaagtccg tcacgccggt gctcgaggtc cgctcgcgcc
     2761 gggtcggcgg cgccaactac caggtccccg tcgaggtgcc gccgcgccgc ggtcgcacgc
     2821 tcgcgctccg ctatctcgtc acgtacgcgc gtgagcgccg cgagaagggg atgcagtcga
     2881 agctcgcggc ggagctcatg gacgcgatca accagcaggg cggcgcgttc aagcgcaagg
     2941 acgacatgta ccggatggcg caggcgaaca aggcgttcgc ccactaccgc tggtgatgag
     3001 atgagcgcga cgatggcatc acaggtcggg ctcgaccgcg tccggaacat cgggatcatg
     3061 gcccacatcg acgcgggcaa gaccacgacg accgagcgga tcctctatta caccggccgc
     3121 acccacaagc tgggcgaggt gcacgagggc gccgcgacga tggactggat ggcgcaggag
     3181 caggagcgcg gcatcaccat cacgtcggcg gcgacgacgg cgttctggcg cgaccaccgc
     3241 atcaacatca tcgatactcc cgggcacgtg gacttcacgg tcgaggtgga gcgctcgctc
     3301 cgtgtgctcg acggcgcggt cgccgtgttc gactccgtcg ccggcgtcca gccgcagtcg
     3361 gagacggtct ggcgccaggc cgaccgttac ggggtgccgc gcatcgcgtt catcaacaag
     3421 atggaccgca ccggcgccga cttcgacgcg gccgtccagt cgatgcgcga ccggctcggc
     3481 gcgaacccgg tgccgatcca gatcccgatc ggccaggagg ac
//
LOCUS       DASPGN010000451         4103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_435109, whole genome shotgun sequence.
ACCESSION   DASPGN010000451 DASPGN010000000
VERSION     DASPGN010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435109"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2113
                     /locus_tag="VD788_13175"
     CDS             <1..2113
                     /locus_tag="VD788_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77263.1"
                     /translation="ICTNMILGVSGASLGIGLGLTATAGSTLVTADWDASGDAAPAAG
                     AAAAVLYRHAPQQGPDEPVVGPFVADNYAGGADQDTASVTLDDFVYYLNALQLGLTAN
                     FEFGGILSPIPDLGGFTIFNLIIDGNQFGIPIGQHAGTSGIDIPIDVDNYALDLDVSA
                     ASSDPDQVVDQKTQRIKPGEFAPYEVRVNNLGSVAGAFDNFARQFSNEPDQSLPFAFV
                     VNQNTDFDCVDAGGQIYRGYPYDGVADDCYDGAGQVRADRAERINEDPVGPDGALESA
                     RDEDGDGVADEDPVDEWRSEPDETMFANRVVLDVPAHASSNPPGSGSSESLILSVSPF
                     RHPLTAPGDYAFRVTADSVEARALGLAATDPSGNARLGAVDVGFIRIESFFEPGIAVE
                     PAGRSAKPGVDQSYQLEASNQGNAEDTVRLVTQIADFNQAGCGLATLGNDPLCPYRAV
                     PTAIPAATWTTLGSLPGAFGPLQPLETDPRQLLVRVPRDWAGMTETTYQIRFTVTSEA
                     DTAEPAASNFVVVEQTVQPTMESMTRFIRLEVDELIAEIELAAGMGFGTGGVSPILIH
                     PVLLHVDNALEETLAGDLGGAAASHRTAIRAMEAFMRALAGGGSKSVPMQVGNDWAER
                     GAAILADLAVAEASPIASAPLAAAQPARAGVARSGSRVVVGSDTGPAGVFADTGGTSG
                     EEPEPAAPRERKLRRGGRAGR"
     gene            complement(2202..3965)
                     /locus_tag="VD788_13180"
     CDS             complement(2202..3965)
                     /locus_tag="VD788_13180"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HXV77264.1"
                     /translation="MAARIGAVHVATTRRRYKGKLYESHLLRRTYREAGKVKHQTLGN
                     ISHLPEAVIEMVRAALRGEHFVPAAASLQILRSLPHGHVAAVVGSLRGLGLETWLGSR
                     PSRQRDLVVAMIAARVIGPASKLATARGLSAETACMSLGGWLGLGAVDEEELYGAMDW
                     LLEKQPRLEGQLAERHLNEGALVLYDVSSSYYTGTHCSLAKFGHSHDGKRDFPQILYG
                     LLCNDEGCPVAVEIFEGNVGEPKTLRVQIQKVRQRFGLRRVVWVGDRGMITGARIREE
                     LEPAGLDWITALRAPAIRQLAEAGLVQPSLFDERDLAEIHSPDYPGERLIACRNPFLA
                     AERAHKREELLKATEREIEGIARATRRPKRSLRGADRIGLRVGKVINRYKMEKHFRLT
                     ITDTSFSYERDREHIAAEAALDGIYIIRTSVSEDVYNAPQTVRAYKNLSRVERAFRCL
                     KTVDLKVRPIHHRLAERVRAHVFLCMLAYYVEWHMRRVLASMLFDDEDRQAAEASRRS
                     VVAPAVRSAKAEAKASTKRTDEGQPVHSFRTLLEDLATITKNRVRPRGAQTAAAEFDL
                     VTTPTPLQQRALSLLGVSLGP"
BASE COUNT          660 a         1467 c         1339 g          637 t
ORIGIN      
        1 gatctgcacc aacatgatcc tcggcgtcag cggggcgagc ctcggcatcg gcctggggct
       61 gacggcgacg gccgggtcga cgctggtcac cgccgactgg gacgcctccg gcgacgcggc
      121 gcccgcggcc ggcgcggcgg ccgcggtgct ctaccgccac gcgccacagc aagggccgga
      181 cgagccggtc gtcggcccgt tcgtcgcgga caactacgcc ggcggcgccg accaggacac
      241 ggcgagcgtc acgctcgacg atttcgtcta ctacctcaac gcgctgcagc tcgggttgac
      301 ggccaatttc gagttcggtg gcatcctcag cccgattccc gacctcggcg ggttcaccat
      361 cttcaatctg atcatcgacg gcaaccagtt cggcatcccg atcggccagc acgccggtac
      421 gagcgggatc gacatcccga tcgacgtcga caactacgcg ctcgatctcg acgtcagcgc
      481 ggccagctcc gatcccgacc aggtcgtcga ccagaagacg caacgaatca aacccggcga
      541 gttcgcgccg tacgaggtcc gggtgaacaa cttgggcagt gtcgccggcg ccttcgacaa
      601 cttcgcgcgc cagttctcga acgagcccga ccagtcgctg ccgttcgcgt tcgtcgtcaa
      661 ccagaacacc gacttcgact gcgtcgacgc cggcgggcag atctaccgcg gctacccgta
      721 cgacggcgtg gccgacgatt gctacgacgg ggcggggcag gtgcgggccg accgtgccga
      781 gcggatcaac gaggatccgg ttggtcccga cggcgcgctc gagagcgcgc gcgacgaaga
      841 cggcgacggc gtggccgacg aggacccggt cgacgagtgg cgcagcgagc ccgatgagac
      901 gatgttcgcc aaccgcgtgg tgctcgacgt gccggcgcac gcctcgtcga acccgccggg
      961 cagcggctcg tccgagtcgc tgatcctgtc ggtcagtccg ttccgccatc cgctgacggc
     1021 gccgggcgac tacgcgttcc gcgtgaccgc ggacagcgtc gaggcgcgag cgctcggcct
     1081 ggccgcgacc gacccgtcgg gcaacgcgcg tctcggcgcc gtcgacgtcg gtttcatccg
     1141 catcgagtcg ttcttcgagc ccgggatcgc cgtcgagccg gccggtcgct cggccaagcc
     1201 gggcgtcgac cagtcctatc agctcgaggc gtcgaaccag gggaacgccg aggacacggt
     1261 gcgactcgtg acgcagatcg ccgacttcaa ccaggccggt tgcgggttgg ccacgctcgg
     1321 caacgacccg ctgtgcccgt accgcgccgt gccgaccgcg attccggccg cgacgtggac
     1381 cacgctgggc agcctgcccg gggcgttcgg tccgctgcag ccgctcgaga ccgatccgcg
     1441 gcagcttctc gtccgggtcc cgcgcgactg ggccgggatg accgagacga cctaccagat
     1501 ccggttcacc gtgaccagcg aggccgacac ggccgagccc gcggcgtcga acttcgtcgt
     1561 cgtcgagcag acggtgcagc cgacgatgga gagcatgacc cgcttcatcc gcctggaggt
     1621 cgacgagctg atcgcggaga tcgagctggc cgccggtatg ggcttcggca cgggaggcgt
     1681 ctcgccgatc ctgattcacc cggtgctgct gcacgtcgac aacgcgctcg aggagaccct
     1741 cgcgggcgat ctcggcggcg ccgccgcgag ccaccgcacc gcgatccgcg cgatggaggc
     1801 gttcatgcga gcgctggccg gaggcggcag caaatcggtg ccgatgcagg tcggcaacga
     1861 ttgggccgag cgcggcgcgg cgatcctggc cgacttggcc gtcgccgaag cgagcccgat
     1921 cgcgtcggcc ccgctcgccg cggcacagcc ggcgcgcgcc ggcgtcgcac ggtccggcag
     1981 ccgagtcgtc gtcggcagcg acaccggccc ggcgggcgtc tttgccgaca ccggcggcac
     2041 gtcgggggag gaaccggagc cggcggcgcc gcgcgagcgc aagctgcggc gcggcggccg
     2101 cgcggggcgc tgacgccccg ttagcccgaa gttccccggc gcggcatgcg taagtctgtg
     2161 agagagcggc ggttcaagtg cacctggatg accggttctg gctacgggcc caaggatacg
     2221 ccgagcaagc tcaaagctcg ttgctgaagc ggagtcggcg tggtgaccag gtcgaactct
     2281 gcggcagcgg tttgggcccc ccgaggccgt actcggttct tggtgatcgt ggccaggtct
     2341 tcgagcaacg tgcggaagct gtgcacgggc tgcccttcgt ccgtgcgctt ggtcgaagcc
     2401 ttggcctcgg cttttgctga acgcaccgcc ggcgcaacca ccgagcgccg cgaggcctca
     2461 gccgcttgtc ggtcctcgtc gtcaaacagc atcgacgcca agactcgacg catgtgccac
     2521 tccacatagt aggccagcat gcacaagaac acgtgcgccc gcacccgctc ggccagccgg
     2581 tggtggatcg gccgcacctt caggtccacc gttttcagac agcgaaacgc ccgctccacg
     2641 cgagacagat tcttgtacgc ccgcaccgtc tgcggagcgt tgtatacgtc ctcggacacc
     2701 gaggtcctga tgatgtagat gccgtcgagc gcggcttcag cggcgatgtg ctcccggtcg
     2761 cgctcgtagg aaaaactcgt gtccgtgatc gtcagccgga agtgcttctc catcttgtag
     2821 cggttgatca ccttgcccac gcgcaggccg atccgatccg ctccccgcaa cgaacgcttc
     2881 gggcgccgag tcgctcgagc gatcccctcg atctcacgct cggtcgcctt gagcaactcc
     2941 tcacgtttgt gcgcccgctc ggccgccaga aacggattgc ggcaggcaat caggcgctcg
     3001 cccgggtaat ccggcgaatg gatctctgcc agatcccgct cgtcaaacag cgacggctgc
     3061 accagacccg cctcggccag ctgccggatg gccggcgcac gcagggccgt gatccagtcc
     3121 agccccgcag gctcgagctc ctcccggatc cgtgccccgg tgatcatccc ccgatcgccg
     3181 acccacacca cgcggcgcaa gccgaagcgc tggcgtacct tctggatctg cacgcgcaga
     3241 gtcttgggct ctccgacgtt accctcgaag atctccacgg ccaccggaca accttcgtcg
     3301 ttgcacagca ggccgtagag gatctgtgga aagtctcgct tgccatcatg actgtgcccg
     3361 aacttcgcca gcgagcagtg cgtgcccgtg tagtagctcg agctcacgtc gtagagcacc
     3421 agcgcaccct cgttcaagtg cctctcggcg agctggccct cgaggcgcgg ctgtttctcg
     3481 agcagccagt ccatcgcccc atacaactct tcctcgtcca ccgcgccgag gcccagccac
     3541 ccacccagcg acatgcacgc cgtctcggcc gaaagcccac gggccgtcgc caacttcgac
     3601 gccggcccga tcacccgagc cgcgatcatg gccaccacga gatcacgctg gcgcgagggc
     3661 cgggagccga gccaggtctc gaggcccagg ccgcgcaggc tcccgaccac cgcggccaca
     3721 tgcccgtgcg gcagactgcg caggatctgc aggctggctg cagccggtac gaagtgctcc
     3781 ccgcgcagtg cggcgcgaac catctcgatc acggcctcgg gcagatgaga gatgttgccc
     3841 aaggtctggt gcttgacctt gccggcttcc cgataggtcc gtcgaaggag gtggctctcg
     3901 tacagtttgc ccttgtagcg gcgtcttgtt gtggctacgt ggacagcgcc aattctcgcg
     3961 gccatggtcg ctattctgcc gcagcatgcc cggaagtgtc aagccatatt agtggctaca
     4021 tttcatccgg acgcgctctc tccaacccgc actccttcaa ccacttgcac cagcatcagc
     4081 cccaatcgcc ccgggaactt cgg
//
LOCUS       DASPGN010000452         6966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1487838, whole genome shotgun sequence.
ACCESSION   DASPGN010000452 DASPGN010000000
VERSION     DASPGN010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487838"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VD788_13185"
     CDS             complement(<1..595)
                     /locus_tag="VD788_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77265.1"
                     /translation="MRVPPSDREIQDYVDGRMPPDERRGFERRMAEDERIAARVRELR
                     AVGAALRDEQLGLPPEFYSRLRQRFEAGRGRQLGWRRVVSWEAAGLATAAALLVLVFV
                     PHLLERSVDSPYSDRTVEPRPRDGGDEKTETDRVAGGPAADVRGNAPEAASPPPVRST
                     DELRLDAGERKPVPAREQQGSPAPSRPRALARMEASAT"
     gene            complement(592..1221)
                     /locus_tag="VD788_13190"
     CDS             complement(592..1221)
                     /locus_tag="VD788_13190"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV77266.1"
                     /translation="MSERSSIGGRREPAADSGVGTDETALLAAIRAGDADAFDRVARE
                     YAPRLYRMALRLTGRREEAEDLVQETLLRTLPALRGFAGRARLSTYLFRALGNLWKNR
                     VRSRKRSRLVAWLRVGDDAEADGDPVSVPVDPSPSAEERLSALDRAEQIRRAVLRLDA
                     DRRLTLLLREVEELSYEEIARMTGVPVGTVRSRLARARSELRRLLGETT"
     gene            complement(1277..2233)
                     /locus_tag="VD788_13195"
     CDS             complement(1277..2233)
                     /locus_tag="VD788_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77267.1"
                     /translation="MLAMTGGARTLVWSLLVAVVVTTELPGQSALPQPLPRPKQPPKV
                     PEGATEYDSVLAPSDAEWGLDPEFLDPLFAKAAVYRDYMRRFTCTETTRVAEYDGSGE
                     VKKEKTQEYGYLLIQDEIDHTIKEYRGEFARDGTLRSGDVKDEEPFPAAYAWVFLFSR
                     FHEPYFSFRFITERFDGFDWVYEIQFRGSLPFRTGKDIREWEGTVFVDAVTHSPLRIV
                     AEPAGQHERIEARFREWNSSFNILGMRTAPKPLGYRATVELRHRDREAELTLPTTMRY
                     DTFTAVSLTDRMPVRSSARQYSDYRIFGATADPEGYGVPSTE"
     gene            2369..3469
                     /gene="dnaJ"
                     /locus_tag="VD788_13200"
     CDS             2369..3469
                     /gene="dnaJ"
                     /locus_tag="VD788_13200"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXV77268.1"
                     /translation="MPTRAKKDYYEILGVGRDADEKAIKAAYRRLARKYHPDLNKGDK
                     RSEERFKEVAEAFAVLSDTEKRARYDRGGHEAFGAGFDPFSGFDARQFDFGFGSGGIE
                     ELLGQLFGGRFGGTHARTTPRAARGQDLRFETRIAFLDAVRGATIDLVVPRQVRCQTC
                     AGSGLRPGQSESTCPACSGSGRQEREQRGMRVSVGCTTCGGRGRLPGAPCDSCGGRGR
                     VPSEERVKVRIPPGVADGDTLRIAGKGDAGTSGAPPGDLLLRIDVDEDARFRREGHDL
                     ICDVPVGISRAALGGEVDVPTLNGPATIKLPQGTRSGQRFRLRGKGVPASGGRPAGDL
                     FAVIQIHPPRKLEGRSRELIEEFDRLNPDGPR"
     gene            3466..3927
                     /locus_tag="VD788_13205"
     CDS             3466..3927
                     /locus_tag="VD788_13205"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HXV77269.1"
                     /translation="MTVPSSASALALLHPACVHFPVVLLSVGGVCEATGYLLGRESAI
                     RTGGWLTIVGTLTLLPTMLTGYLAAYSVSVPPAAERTLAMHELNGWLVLALFVGLLFW
                     KGWYRGSVPTGQRRPYAIVQLAAVGLVVYGAVLGGELVYLHGMGVRAVGGP"
     gene            complement(3935..4840)
                     /locus_tag="VD788_13210"
     CDS             complement(3935..4840)
                     /locus_tag="VD788_13210"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HXV77270.1"
                     /translation="MNWLDRAISLSLPLVPKPIVRRFSGRYIAGETIDEALAVVRALR
                     AEGAMTTIDILGEFISSREEARANTEAYSELLGRIRSERIDQTNVSVKLTALGLLLDP
                     ELCLTHMRTLFDQVSGAGDFIRIDMEDSSCTNRTLDVYRALRGEYGERVGIVLQARLR
                     RTLEDIDEIGRQPANVRLCKGIYLEPRDIAYTGAELIRRNFVRSLDRLFELGCYVGIA
                     THDELLVWESERLIRERRLSADDYEFQMLLGVDEQLRRILIGSGHRLRVYVPYGPNWY
                     AYSVRRLRENPQIAGYAFRSILRSG"
     gene            complement(4863..5090)
                     /locus_tag="VD788_13215"
     CDS             complement(4863..5090)
                     /locus_tag="VD788_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5058..5060),aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HXV77271.1"
                     /translation="MRIRIEYCSEUNYKPRAAGLAAELTEQFAADTELVASHGGVFEV
                     WCDGALLFSKTALSRFPEAGEIVRLIRARDE"
     gene            5209..6603
                     /locus_tag="VD788_13220"
     CDS             5209..6603
                     /locus_tag="VD788_13220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV77272.1"
                     /translation="MTSLTFCGATGTVTGSRFLLEHHGFRMLVDCGLFQGLKQLRERN
                     WSAPPFAPAELDLVVLTHAHLDHTGYFPRLARLGYDGPVLATSATCSLCGILWPDSGH
                     LQEEDARFANKRGFSKHKPALPLYTEEEARIALDRIRPRVFGSTLRVHDEIEIRFHPA
                     GHILGAAFVEVRIDVGRGKTRTVLFSGDLGRPRQPILPDPTRLPPADVLLLESTYGNR
                     DHPADDPKDRLAAVIRETTEAGGTVLIPAFAVGRTTHLLYLLRSLQRENRLPTDIPIH
                     VDSPMAIDAIRTLVEHDEAHDPEMRALVANGDDPLGLRHVRLAATVEQSKALNDLRYP
                     AVIISASGMATGGRVLHHLAYRLGEHRTTVLFVGYQAAGTRGRSLQDGAKTVRIHGRE
                     VRVRARVETVDGLSAHADRSEIMSWLGAAERAPGRVYLVHGEPPASRALAERIRGTTS
                     LEVHEPSYLQRVEI"
     gene            6603..6872
                     /locus_tag="VD788_13225"
     CDS             6603..6872
                     /locus_tag="VD788_13225"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXV77273.1"
                     /translation="MPLWVRTLLVTALPAEAEPLLDAHRLHLRELQRAGKLRIAGEFP
                     GGDGFLEIFEADDRREAETIAARSPLVEAGQASWLLRRWDELTFD"
BASE COUNT         1063 a         2415 c         2371 g         1117 t
ORIGIN      
        1 cggtcgcact cgcctccatc cgggcgagcg cccggggtcg cgacggagcg ggagatccct
       61 gttgctcccg cgccggcacg ggtttcctct cacccgcgtc gagtctgagt tcgtcggtgg
      121 agcggactgg cggtgggctt gccgcttcgg gtgcgttccc gcgcacgtcg gctgccggac
      181 caccggccac gcggtccgtc tcggtcttct cgtctccacc atcccgcgga cgcggctcta
      241 ccgtccggtc cgagtacggc gagtcgacgc tcctctccag caggtgcggc acgaagacga
      301 gtacgagcaa cgccgccgcg gtcgccagcc cggctgcttc ccacgatacg acgcggcgcc
      361 agccgagctg ccgaccgcga ccggcttcga agcgctgccg caggcgcgag tagaattcgg
      421 gtggcagccc aagctgctcg tctctcagcg cggctccgac cgcgcgcagc tcgcgtacgc
      481 gcgccgcgat ccgctcgtcc tcggccatcc gccgttcgaa cccccggcgc tcgtccggcg
      541 gcatccgtcc gtcgacgtag tcctggattt cgcgatcgct cggcgggact ctcacgtcgt
      601 ttctccgagc agtcttctca gttccgaccg cgcacgcgcc agtcgcgaac gcaccgtacc
      661 gacggggacg cccgtcattc gcgcgatctc ctcgtaggac agctcttcga cctcgcgcag
      721 cagcagggtc aagcggcgat ccgcgtccaa tcgcagcacc gcccgacgaa tctgctcggc
      781 acggtcgagg gccgagaggc gttcctcggc gctcggtgac ggatcgacgg gcaccgacac
      841 cggatcgcca tcggcctcag cgtcgtcgcc gacgcggagc caggcgacga gccgcgagcg
      901 cttgcgcgat cggacccggt tcttccacag gttgccgaga gcgcgaaaca ggtaggtcga
      961 cagcctcgca cgacccgcga agccacgcag cgccggcagg gttcgcaaca acgtctcctg
     1021 cacgaggtcc tccgcctcct cgcgccggcc ggtcaggcgc agcgccatgc gatagaggcg
     1081 tggcgcgtac tcccgcgcga cgcgatcgaa cgcgtccgca tcgcccgcgc ggatcgcggc
     1141 gagcagagcg gtctcatccg taccgacacc ggaatcggca gcgggttccc gcctcccgcc
     1201 gatcgagctg cgctcgctca cgacgtatag gttcgtgcgg agcggtctcc ggggtcaagt
     1261 cggtccgcgg gcgaggctac tccgtcgagg gtacgccgta tccctcggga tcggcggtcg
     1321 cgccgaatat gcggtagtcc gagtactgcc gcgccgagga ccggacgggc atccggtcgg
     1381 tgagcgaaac ggcggtgaac gtgtcgtagc gcatcgtcgt gggcagggtc agctcggcct
     1441 cgcgatcgcg gtgccgcagc tcgacggtgg cccgatagcc cagcggtttc ggcgccgtac
     1501 gcataccgag gatgttgaac gagctgttcc actcgcggaa tcgtgcctcg atccgctcgt
     1561 gttgtcccgc cggctcggca acgattcgca gcggcgagtg ggtgaccgcg tcgacgaaca
     1621 ccgtaccctc ccattcgcga atgtccttgc ccgtccggaa cgggagcgag ccacggaact
     1681 ggatctcgta gacccagtcg aagccgtcga accgctcggt gatgaaacgg aacgagaagt
     1741 acggctcgtg gaagcggctg aacaggaaga cccaggcgta ggccgcgggg aacggctcct
     1801 cgtccttgac gtcgccgctc ctcagcgtcc cgtctcgagc gaactcgccg cggtactcct
     1861 tgatggtgtg gtcgatctcg tcctggatca gcaggtagcc gtattcctgc gtcttctctt
     1921 tcttgacctc gcccgatccg tcgtactcgg cgacccgcgt cgtttcggtg caggtgaaac
     1981 gacgcatgta gtcccggtag accgcggcct tggcgaacag cgggtcgaga aactcggggt
     2041 cgagacccca ctccgcatcg gacggcgcga gcacgctgtc gtattcggtc gccccctcgg
     2101 gcaccttcgg cggctgcttg ggacgaggca acggttgggg caaggcggat tggcccggca
     2161 gctcggtggt aacgaccacg gccaccagca gcgaccaaac gagtgtccgt gcccctccgg
     2221 tcatcgcgag cattctacct tgtccggcga ttcgcgccgg ttcgacccgc ttgacatcgg
     2281 cgttccgccg ttctaacaac tggacgcggc accaggctcc ctgaccggga cgaggtgcgg
     2341 gtctgttcag ccggaggttc cagcgaccgt gccgacgaga gcgaagaagg actactacga
     2401 gatcctcggc gtcggtcgcg acgcggacga gaaggcgatc aaggctgcct atcgacgcct
     2461 ggcgcgcaag taccacccgg atttgaacaa aggcgacaag cggtccgagg aacggttcaa
     2521 ggaagtggcg gaggccttcg cggtcctgtc cgataccgag aaaagggcac gctacgaccg
     2581 cggcgggcac gaggcgttcg gcgccggctt cgatccgttc tccggtttcg atgcccggca
     2641 gttcgacttc ggtttcggtt ccgggggcat cgaggagctg ctcggacaac tgttcggcgg
     2701 ccggttcggc gggacgcacg cccgcaccac gccgcgcgcg gcccggggtc aggatctccg
     2761 cttcgagacg cgcatcgcgt tcctcgatgc ggtccggggc gccacgatcg acctggtcgt
     2821 gccgcgccag gttcggtgcc agacctgtgc cggttccggt ctgcgcccgg gtcagagcga
     2881 gtcgacctgc ccggcctgtt ccggcagcgg ccggcaggag cgcgagcagc gcgggatgcg
     2941 cgtctcggtc ggttgcacga cctgtggagg acgcggtcgc ttgccgggcg ccccgtgtga
     3001 ttcctgcggc ggtcgaggac gcgtcccgag cgaagaacgg gtcaaggtgc gcatccctcc
     3061 cggcgtcgcc gacggcgaca cgcttcgcat cgccggcaag ggcgacgccg gcacgagcgg
     3121 ggctccgccc ggtgatctgc tgctgcggat cgacgtcgac gaggacgcgc ggttccggcg
     3181 agagggccac gacctgatct gcgacgtacc cgtcgggatc tcgcgcgcgg cgctcggagg
     3241 cgaggtcgac gtgccgacgc tcaacggccc ggcgacgatc aagctgccgc agggaacgcg
     3301 cagcggccag cgcttccgtc ttcgcggcaa gggtgttccc gcctccggcg gccggccggc
     3361 cggggacctg ttcgccgtga tccagatcca tccgccccgc aagctcgaag gccggtcgcg
     3421 ggagctgatc gaggagttcg accggttgaa tccagacggt ccccggtgac cgtgccgtcg
     3481 tccgcctcgg ccctggcctt gttgcacccg gcgtgcgtgc actttcccgt cgtgctgttg
     3541 agcgtcggcg gcgtgtgcga ggcgaccggc tacctgctcg ggcgggaatc ggcgatccgc
     3601 accggtggct ggctgacgat cgtcggcacg ctgacgctgc tgccgacgat gctgaccggc
     3661 tacctggccg cctactccgt ttcggttcca cccgccgccg agcgtacgct cgcgatgcac
     3721 gagttgaacg gctggctggt gctcgcgctg ttcgtcgggt tgttgttctg gaaggggtgg
     3781 taccgcggca gcgtgcccac cgggcagcgc cggccgtacg cgatcgtgca gcttgcggcg
     3841 gtcggcctgg tcgtctacgg cgcggtcctc ggcggcgagc tggtctacct gcacgggatg
     3901 ggcgtccgcg cggtcggcgg tccgtagctc cgcctcagcc cgaacggagg atcgaccgaa
     3961 acgcgtagcc cgcgatctgt ggattctccc gcaggcggcg caccgagtag gcgtaccagt
     4021 tcggaccgta cggcacatag actcgcaggc ggtgaccgct gccgatcagg atccgtcgca
     4081 actgctcgtc gacgccgagc agcatctgga actcgtagtc gtccgcgctc aaccgccgct
     4141 cccggatcag ccgttccgac tcccacacca gcaactcgtc gtgggtcgcg atgccgacgt
     4201 agcaacccaa ttcgaacaag cgatcgagcg agcggacgaa attgcggcgg atcagctccg
     4261 cgccggtgta ggcgatgtcg cgcggctcga gatagatgcc cttgcacaac cggacgttgg
     4321 cgggttggcg gccgatctcg tcgatgtcct cgagcgtccg gcgcaagcga gcctgcagca
     4381 cgatcccgac ccgctcaccg tattctccgc gcagcgcgcg atagacgtcg agcgtgcggt
     4441 tggtgcacga cgagtcttcc atgtcgatac ggatgaagtc gccggcgccc gagacctggt
     4501 cgaaaagcgt gcgcatgtgg gtcaggcaca gctccggatc gagcagcaag ccgagtgccg
     4561 tcagcttcac cgagacgttc gtctggtcga tccgttccga gcggatgcgg ccgagcagct
     4621 ccgagtaggc ctcggtattg gcccgcgcct cctcccgcga cgagatgaac tcgcccagga
     4681 tgtcgatcgt cgtcatcgca ccctcggcgc gcagcgcgcg aacgacggct agcgcttcgt
     4741 cgatcgtttc tcccgcgatg tagcggcccg agaaccggcg cacgatcggt ttcggcacca
     4801 gcggcaggga gagcgagatg gcgcgatcga gccagttcat cggtgattcg gccggcccgg
     4861 agctactcgt cgcgggcacg gatcaggcga acgatctccc ccgcttcggg aaagcgggac
     4921 agggccgtct tcgagaaaag tagcgccccg tcgcaccaga cctcgaacac acccccgtgt
     4981 gacgcgacca gctcggtgtc cgccgcgaac tgctcggtca actcggccgc cagaccggcg
     5041 gctcggggct tgtagtttca ctcgctgcag tattcgatgc gaatccgcat tgccgactcc
     5101 ggtccggtcc gacccgggtg ctaccgagag ccgtcatcct agcaagcggc ccgtccttta
     5161 ccaagacgtc ctccgacggg cggcggcctg tgttagcctg ggtggccgat gacgagcctg
     5221 accttctgcg gcgccaccgg caccgtgacg ggatcgcgct tcctgctcga gcaccacggt
     5281 ttccgcatgc tcgtcgattg cgggctgttc cagggtctca agcagctcag ggagcgcaat
     5341 tggagcgccc caccgttcgc accggccgag ctcgatctgg tcgtgctgac ccacgcccac
     5401 ctcgaccaca ccggttactt cccgcgactc gcgcggctcg gttacgacgg accggtgctc
     5461 gccacctcgg ccacctgcag cctgtgcggc atcctctggc cggacagcgg tcacctgcag
     5521 gaagaggacg cgaggttcgc caacaaacgc ggtttctcca agcacaaacc cgcgctcccg
     5581 ctctacaccg aggaggaggc ccggatcgcg ctcgatcgga tccgaccccg cgtcttcggc
     5641 agcacgctgc gcgtccacga cgaaatcgag attcggttcc atccggccgg tcacatcctc
     5701 ggcgcggcgt tcgtcgaggt gcggatcgac gtcggccgcg gcaagacgag gacggtgctc
     5761 ttctcgggag acctcggacg cccgcgccag ccgatcctgc ccgatccgac ccggctgcca
     5821 ccggccgacg tgctgctgct cgaaagcacg tacggcaacc gcgaccaccc ggcggacgat
     5881 ccgaaagatc ggctggcggc ggtgatccgc gagacgaccg aagcgggcgg gaccgtgctg
     5941 atcccggcgt tcgcggtcgg gcggacgacc cacctgctct acctgctgcg ctcgctccaa
     6001 cgcgagaacc ggctgcccac cgacattccg atccacgtcg acagcccgat ggcgatcgac
     6061 gccattcgaa ccctggtcga gcacgacgag gcccacgatc cggagatgcg ggcgctcgtg
     6121 gccaacggcg acgacccgct cggcttgcgg cacgtccggc tggccgccac ggtcgagcag
     6181 tcgaaggcgt tgaacgacct acgttacccg gcggtcatca tctcggcctc cggcatggcg
     6241 accggcgggc gggtgctgca ccatctggcc tatcggctcg gcgagcaccg gacgaccgtg
     6301 ctgttcgtcg gttatcaggc ggcgggaaca cgcggtcgca gtctgcagga cggcgcgaag
     6361 acggtgcgga tccacgggcg ggaggtccgg gtgcgtgcgc gagtcgagac cgtcgacggt
     6421 ctctcggccc acgccgaccg gagcgagatc atgagctggc tcggcgccgc cgagcgggcg
     6481 ccggggcgcg tctatctggt gcacggcgaa ccaccggcca gtcgggcgtt ggccgagcgc
     6541 atccgcggta cgacctcgct cgaggttcac gagccgagtt acctgcaacg agtcgagatc
     6601 tgatgccgtt gtgggtccga acactgctcg tcaccgctct gccggccgag gccgagccgc
     6661 tgctcgacgc ccatcggctc cacctgcgcg agctgcagcg cgcggggaag ttgcgaatcg
     6721 ccggcgagtt cccgggcggc gacggcttcc tcgagatctt cgaagccgac gatcgccgcg
     6781 aggccgagac gatcgccgct cggagtccgc tcgtcgaggc cgggcaggcg agttggctgc
     6841 tgcggcggtg ggacgagctc accttcgatt gagcgaccgg cgactcaggc ggactccgat
     6901 gccgggacgg gcggctcgac ccagcggccg tgcttcttga tcagctcgac cagtcgttcg
     6961 tcggca
//
LOCUS       DASPGN010000453         9458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1254236, whole genome shotgun sequence.
ACCESSION   DASPGN010000453 DASPGN010000000
VERSION     DASPGN010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9458
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254236"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VD788_13230"
     CDS             complement(<1..247)
                     /locus_tag="VD788_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXV77274.1"
                     /translation="MERTLAIIKPDGVESKVVGKILARIEDEGFGIVAMRLLRLSRRQ
                     AEGFYAVHRQRPFFADLTAFISSGPVVVLVLERRNAIA"
     gene            complement(264..1133)
                     /gene="sucD"
                     /locus_tag="VD788_13235"
     CDS             complement(264..1133)
                     /gene="sucD"
                     /locus_tag="VD788_13235"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXV77275.1"
                     /translation="MAVLVDGDTRLLVQGLTGREGTFHTKQAIEYGTRVVAGVTPGKG
                     GQTHQGIPVFDTVAQAVEATGANASVIFVPPPFAADAIMEAAAAGVALVVCITEGIPT
                     LDMLRVWNYLDGRATRLIGPNCPGIITPGACKIGIMPGHIHAAGPVGVVSRSGTLTYE
                     AVDQLTKLGIGQSTCVGIGGDPIVGTDFIDMLTLFQQDDRTEAIVMIGEIGGTAEEQA
                     AKFVERQVDKPVVSFIAGQTAPPGRRMGHAGAIISGGKGTAADKMQALRAAGIHVVET
                     PAEIGDTVKRVIG"
     gene            complement(1135..2358)
                     /gene="sucC"
                     /locus_tag="VD788_13240"
     CDS             complement(1135..2358)
                     /gene="sucC"
                     /locus_tag="VD788_13240"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HXV77276.1"
                     /translation="MNIHEFQAKALLRDHGVPVPRGEVARSADRVCAIAERLGPTVVV
                     KAQIHAGGRGKGRFRREDEGSPLMRPGTDEPLGGVVVVDGPERAEQVAAMMLGNVLVT
                     RQTGAEGRVVRQLLIEEGMQIADEYYLALLLDRAAKSITFIVSAEGGMEIEQVAAERP
                     DAILKVSVDTAAGYSPWIGRRIAYFLGMEPAAAAAAGKLAESLYACFIRTDASLVEVN
                     PLVRTKDGRVLALDAKMNFDDNALYRQTSVAALRDLSEEDPLEVEASKHGLNYIKLDG
                     TVGCMVNGAGLAMGTMDIIKHHGGNPANFLDVGGGATSEMVANAFRILLGDPHVRAVL
                     INIFGGIMRCDIVAEGVVNAAREVGVTMPVVVRLAGTNVERGRQILRESGLRFQVAGG
                     MDEAARLVVAAAGRG"
     gene            complement(2488..3153)
                     /locus_tag="VD788_13245"
     CDS             complement(2488..3153)
                     /locus_tag="VD788_13245"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXV77277.1"
                     /translation="MATSSAERRARRRALDRMHVLQRRERELRDAGVVHVAGVDEVGV
                     GPLAGPVVAAAVVFDERCRIAGIDDSKRLGAARRIELARTIREVATCWSVFEVAPAEI
                     DRINIYQASLEAMRRALLGLRPCPDHVLVDARRIPDLPFPQEPWVRGDARCYAIAAAS
                     ILAKTARDERMQRYDVEFPGYGFADHKGYATAAHRDAIRRLGPSPIHRRSFVLLPHPS
                     LFD"
     gene            3279..4028
                     /gene="gpmA"
                     /locus_tag="VD788_13250"
     CDS             3279..4028
                     /gene="gpmA"
                     /locus_tag="VD788_13250"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HXV77278.1"
                     /translation="MHKLVLLRHGQSTWNLENRFTGWTDVDLTPQGIAEAEEAGRLLT
                     EGGYLFDVAYTSVLKRAIRTLWIVLDRMDLMWIPVVRHWRLNERHYGALQGLNKAETA
                     SKHGAEQVKIWRRSYATPPPALTDDDERHPGRQPPYSLLDRGEIPLTESLKDTVERFL
                     PYWHGTIAPDLERGRRILICAHGNSLRALVKYLDRISDDEIVALNIPTGIPLIYELDD
                     DLSPIRHYYLGDPERARRAADAVADQARKNP"
     gene            4157..4417
                     /locus_tag="VD788_13255"
     CDS             4157..4417
                     /locus_tag="VD788_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HXV77279.1"
                     /translation="MTFVITEPCIGTKDTACVDVCPVDCIHPTKESPEFEKVEMLYID
                     PDECIDCGACEPACPVEAIFEEDAVPEKWKEYTEKNAAYFRK"
     gene            4479..5795
                     /locus_tag="VD788_13260"
     CDS             4479..5795
                     /locus_tag="VD788_13260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXV77280.1"
                     /translation="MADTRLSRPAGGRRVVVTGLGAVSPNGVGIDRFWCATRQGTSGV
                     SRITEFDVSDYPTRIAGQVRDFDPTRVVSPRDAKHIPRASTLALAAAVEALDDAGIDP
                     DRLDIAERRATAVVVGSGGVGWEFTERQFREYYFGNPKAVSLYTIPSSTPGSISSELS
                     MRFGFRGASHLISTGCTSSSDALGHAFSLIRYRRARMVLAGGTDAPLAPGILRGFCLM
                     KIMTPSWNDEPERASRPFSRDRDGFVLAEGSWMLLLEDLEQALERGATIHAELLGYGA
                     TCEAFHRVRLSEDGEEPARAMELALADAGLDSSQIDYVNVHGTSTELNDRIETRALRL
                     VFGDRMASLPVSSLKSMIGHPQGACGAAGVVASLLAMRDRFIPPTINYDLFDPQCDLD
                     VVPHEGRPGELGAVLCNCIGFGSKNSALVFCATDPGGGSLVDVRRT"
     gene            5799..6515
                     /locus_tag="VD788_13265"
     CDS             5799..6515
                     /locus_tag="VD788_13265"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV77281.1"
                     /translation="MRLVPDRRDDAEWIDRPDNTVADLRAAFGDIGWTHRWLGGRAVL
                     LSALGPYLDRSDGPLRILDVGTGAADLPLAIIAWARRLGRPVEVVGLDRDFGVTSIAA
                     GAAAGCAELTVVCADARRLPFDDRSFDLVTASLFLHHFGPGEWIKLLGGFRRVARRAV
                     VVCDLRRHLLPWVFLAGLARLTRRHPMFVHDAPLSVLRGFTDRELLDAATACGASSVS
                     LRRRWPFRLLLTIRVEGEAA"
     gene            6512..7696
                     /locus_tag="VD788_13270"
     CDS             6512..7696
                     /locus_tag="VD788_13270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXV77282.1"
                     /translation="MRRRSDLLVLGGGPAGSTVAAWAAAAGCAVTVVDQARFPRDKVC
                     GEFLSSEGCRVLERIGLLGGLIAQGAVPINRFRIGDASGRSIEAPLPAPGDPIPRALG
                     VSRALLDRLLLEHAAARGARVLERCRALRPIVEGGRVEGAVVRAIDGRSSAIELRATL
                     VVAADGRRSMLARALHPDSGDPSTTHTGSWFGLKTHVGGAPGELADRVELQLFDGGYV
                     GLAAVDGGRINVCLMTRVAALRACGGSPDRLLSERILANPAVARVLGGSERCGRWHAI
                     GPLAWGPRRPAAAGALFVGDAAGTIDPFSGLGISNALRAAELVSDTVLVAVDRGALEP
                     GAARAYARRWRASFAPLTRRVRVVGSLFERPRLAVPLIGWLRGAGSGWLPRLLAATRA
                     EV"
     gene            7700..9316
                     /locus_tag="VD788_13275"
     CDS             7700..9316
                     /locus_tag="VD788_13275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXV77283.1"
                     /translation="MKRGGWLVAGALLAGALFCSSLGVLWPLAPIDLVVPERQLVRQA
                     LGFLEQRGFLLGGYDSAEWLRVDAPGLDYVERAFGKARADEWIRAGLPLYEYRVAFKR
                     AGDPLVVRVSLHPDRGALGWTAELPRDLAGERLDAVSARTIAQREIEPALGLDLDQWQ
                     ETSFSTELLPARVDHRLVYERQLVEHPELRERLSVVVAGDRVATAVRSIVVPPSAERQ
                     ARRGEAASVALESFGFLLLGIAAAAAFVVFLRRISAGEVRLGRAAGWPVVVFVCFTVA
                     SVLDSFRLFLGWDPLWPRAVSAFRDLLLDSLTQVWVLLVLLAVVAAGDALDRASGANR
                     GRSLWLLARGRVADRSVALASGRGFLIGLACGGVMASIVVLLHQIGVARSSIQPRGFF
                     FYPLNSEAPAITTLLFFFGVSLAEELGYRFFGGTWLEGLTRRRWLAVVVPALVYGLTH
                     TGLDFLPPAEPFWARPLVLTAVGCVWGWAFLRYDALTVVLSHFTADLFIFNWPRLASG
                     EPTAVTLALVTIGVPLLPALAWPWIGSRKG"
BASE COUNT         1345 a         3283 c         3226 g         1604 t
ORIGIN      
        1 gagcgatcgc gttccggcgc tcgagcacca gcacgacgac cggacccgac gagatgaacg
       61 cggtgaggtc ggcgaagaac ggtcgttggc gatggacggc gtagaagcct tccgcctgac
      121 ggcggctcag ccgcagcaga cgcatggcga cgatgccgaa gccctcgtcc tcgatccgtg
      181 ccaggatctt gcccacgacc ttgctctcga ccccgtccgg cttgatgatc gccagcgttc
      241 gttccatgct cccgcctccc cggtcaaccg atgacccgtt tgactgtgtc gccgatctcg
      301 gccggcgtct cgacgacgtg gatgccggcg gcccgcagcg cctgcatctt gtcggccgcg
      361 gtgcccttgc cgcccgagat gatcgccccg gcgtgcccca tccggcgtcc gggcggagcc
      421 gtctgaccgg cgatgaacga caccaccggc ttgtcgactt ggcgctcgac gaacttcgcc
      481 gcctgctctt cggcggttcc gccgatctct ccgatcatca cgatcgcctc ggtccggtcg
      541 tcctgctgga acagcgtcag catgtcgatg aagtcggtgc cgacgatcgg atcaccgccg
      601 atcccgacac aggtcgactg gccgataccg agcttggtca gctgatcgac ggcctcgtag
      661 gtcagggttc ccgaccgtga tacgacgccg accggccccg cggcgtggat gtggcccggc
      721 atgatgccga tcttgcacgc tcccggcgtg atgatccccg gacagttcgg accgatcagc
      781 cgcgtcgcgc gcccgtcgag gtagttccag acccgcaaca tgtcgagcgt cgggatcccc
      841 tcggtgatgc agacgaccaa cgcgacgccg gccgccgcgg cctccatgat cgcgtcggcg
      901 gcgaacgggg gcggcacgaa gatcacgctg gcgttggctc cggtcgcctc gaccgcctgt
      961 gcgacggtgt cgaagaccgg gatgccctgg tgggtctgac cgcccttgcc gggcgtgacg
     1021 ccggcgacca cccgcgtgcc gtactcgatc gcctgcttgg tgtggaaggt cccttcgcgc
     1081 ccggtcaatc cctgcacgag caatcgcgta tcgccgtcga ccagcaccgc cacgctagcc
     1141 cctccccgcc gcggcgacca ccagccgggc ggcctcgtcc atccctccgg ccacctgaaa
     1201 acgcaagccc gactcgcgca ggatctgccg tccccgctcg acgttggtcc cggcgaggcg
     1261 cacgaccacc ggcatcgtga caccgacctc acgcgcggcg ttgaccaccc cctcggcgac
     1321 gatatcgcag cgcatgatgc cgccgaagat gttgatcagc acggcacgga cgtgtgggtc
     1381 gccgagcaga atgcgaaagg cgttggccac catctcgctc gttgcgcctc cgccgacgtc
     1441 gagaaagttg gccgggttac cgccgtggtg cttgatgatg tccatcgtcc ccatcgcaag
     1501 gccggcgccg ttgaccatac aaccgacggt accgtccagc ttgatgtagt tgagcccgtg
     1561 cttcgacgcc tcgacttcca acggatcttc ctcggacaga tcgcgcagcg ccgcgaccga
     1621 cgtctggcgg tagagggcgt tgtcgtcgaa gttcatcttg gcgtcgagcg ccagcacccg
     1681 gccgtccttc gtcctgacca gcgggttgac ctcgaccaac gacgcgtcgg tcctgatgaa
     1741 gcacgcgtag agcgactcgg cgagcttgcc ggcggcggcg gccgcggccg gctccattcc
     1801 gagaaagtag gcgatgcggc gtccgatcca cggcgagtag ccggcggcgg tgtcgaccga
     1861 caccttgaga atcgcgtcgg ggcgctcggc ggcaacctgc tcgatctcca tcccgccttc
     1921 ggccgagacg atgaaggtga tcgacttcgc ggcccgatcg agcaacaacg cgaggtagta
     1981 ctcgtccgcg atctgcatcc cctcttcgat caacagctgg cgcacgacac ggccttcggc
     2041 gccggtctgc cgcgtgacca ggacgttgcc gagcatcatc gccgccactt gctccgcccg
     2101 ctccggcccg tcgacgacga ccacgccgcc gagaggctcg tcggtgcccg gtcgcatcaa
     2161 cggggagcct tcgtcctcgc gcctgaacct gcccttccca cgaccgccgg cgtggatctg
     2221 cgccttgacg acgacggtcg gaccgagccg ctcggcgatc gcgcacaccc gatcggccga
     2281 acgcgcgacc tcaccgcgcg gcacaggaac accgtgatcc cgcagcaggg ctttggcttg
     2341 gaactcgtgg atgttcacgt cttgtctcca ggagaagccg tggtcgatgg agagaaacgc
     2401 ccgtcgcgag cggccgaatt ataggatagc gctcggacga gttccaccgc gcgctcgacc
     2461 atcgcgccgt gcgatcgggc gcctgcttca gtcgaacaac gacgggtgcg gcaacagcac
     2521 gaacgaccgg cggtggatcg gcgacggtcc gaggcgccgg atcgcgtcgc ggtgggccgc
     2581 cgtcgcgtat cccttgtgat cggcgaagcc atagccgggg aactcgacat cgtagcgttg
     2641 catccgctcg tcacgggcgg tcttggccag aatcgaggcg gcggcgatcg cgtagcagcg
     2701 cgcatcgccc cgaacccacg gttcttgggg gaacggcagg tccgggatcc gccgcgcgtc
     2761 gaccagcacg tggtccgggc acggacgcaa cccgagcagg gcgcgccgca tcgcctcgag
     2821 gctggcctgg tagatgttga tccggtcgat ctccgcgggc gccacctcga agacggacca
     2881 gcaggtcgcg acctcacgga tcgtcctcgc caactcgatc cgccgcgccg cgccgagccg
     2941 cttggaatcg tcgatgcccg cgatccgaca gcgctcgtcg aatacgaccg cggccgcaac
     3001 gaccgggccg gccagcggtc cgacgcccac ctcgtcgacg ccggcgacgt ggaccacgcc
     3061 cgcatccctc agctcacgct cgcggcgctg gagcacgtgc atccggtcga gggcgcggcg
     3121 ccgcgcacgg cgctcggcgg aactggtagc caaacccacc tcctgttgcg gccgatcgct
     3181 ccgcgccagc ctacctcgtc tcgaccggcc ggcaaaggct tgacgccggc gcgcgggggt
     3241 ggttcgatag aagatggtgg accgaacggg ggagaccgat gcacaagctc gtcctgctgc
     3301 gtcacgggca gagcacgtgg aacctcgaga accgattcac cggctggacc gacgtcgacc
     3361 tgacaccgca gggaatcgcc gaagcggagg aagcgggccg tctgctcacc gaaggcggtt
     3421 acctgttcga cgtggcctac acgtcggtgc tcaagcgtgc gatccgcacg ttgtggatcg
     3481 tgctggaccg gatggacctg atgtggatcc ccgtcgtgcg tcactggcgg ctcaacgagc
     3541 gacactatgg cgcgctgcag ggcctaaaca aagcggaaac ggcgagcaag cacggcgcgg
     3601 agcaggtgaa gatctggcgc cgcagctacg cgacgccgcc gcccgcactg accgacgacg
     3661 acgagcgcca cccgggtcgc caaccgccct attccctgct cgaccgaggc gagattccgc
     3721 tcaccgagtc gttgaaggac accgtcgagc gtttcctgcc gtactggcac gggacgatcg
     3781 cacccgacct cgagcgcggg cgtcggatac tgatctgcgc ccacggcaac agcctccgcg
     3841 cgttggtcaa gtacctcgac cggatctcgg acgacgagat cgtcgcgctc aacatcccga
     3901 ccgggattcc gctgatctac gagctcgacg acgacctgtc gccgatccgc cactactacc
     3961 tcggcgatcc ggaacgcgca cgccgagccg ccgatgccgt ggcagatcag gcccgcaaaa
     4021 acccgtgagc gtgtacgcgc ggcgagcttg accgaggtca aggaaccgcg ggtaccagcg
     4081 gtctatagtg ctgccggtcg gacggcgcga cggccgaaga gcgagcgatc ggcgtcgccc
     4141 aaggaggacc cgccccatga ccttcgtgat caccgagccc tgcatcggga ccaaggacac
     4201 ggcatgtgtc gacgtctgtc ccgtcgactg catccacccg accaaggaat ctcccgagtt
     4261 cgagaaggtc gagatgctct acatcgatcc cgacgagtgc atcgattgcg gagcgtgcga
     4321 gccggcatgt ccggtcgagg cgatcttcga agaggacgcc gtgcccgaaa agtggaaaga
     4381 gtatacggag aaaaacgccg cctacttccg gaagtagccc actcggccga tcgctcaccc
     4441 gcgttcgtcg tcggctcgcg gatgttagtc tgttgacgat ggcggatacc cgactcagcc
     4501 gcccggccgg cggccgccgc gtcgtcgtca ccgggctcgg tgcggtctcg cccaacggcg
     4561 tcggcatcga tcgtttctgg tgcgcaactc gccaggggac gagcggcgta tcgcggatca
     4621 ccgagttcga cgtctcggac tacccgaccc ggatcgccgg ccaggtgcgc gacttcgatc
     4681 cgacgcgggt cgtctcaccg cgtgatgcga agcacatccc gcgagcctcg accctcgcgc
     4741 tcgccgcagc ggtcgaagcg ctcgacgacg ccgggatcga ccccgatcga ctcgacatcg
     4801 ccgagcgacg agcgacggca gtcgtcgtcg gcagcggcgg ggtcggctgg gagtttaccg
     4861 agcggcaatt ccgcgaatac tacttcggca acccgaaagc ggtcagcctt tacaccattc
     4921 cgtcctcgac accgggctcg atcagcagcg agctgtcgat gcgcttcggc ttccgcggcg
     4981 cgagccactt gatctcgacc ggttgcacct cttcgtcgga cgcgttgggt cacgcgttct
     5041 cgctgatccg ttaccgacgc gcccggatgg tgctggccgg cggtaccgac gccccgctcg
     5101 cgccggggat cctccgcggt ttctgtctga tgaagatcat gacgccgtcg tggaacgacg
     5161 agccggagcg cgcctcccgt ccgttctccc gcgaccgcga cggcttcgtg ctcgccgaag
     5221 gctcgtggat gctgttgctc gaggacctcg agcaagcgct cgagcgcggc gcgacgatcc
     5281 acgccgagct actgggctac ggagcgacgt gtgaggcgtt ccaccgcgtg cgcctcagcg
     5341 aggacggaga agagccggcc cgagcgatgg agctcgcgct cgcggacgcc ggtctcgatt
     5401 cgagccagat cgactacgtc aacgtccatg gcacctcgac cgagctcaac gatcgtatcg
     5461 aaacccgtgc gctgcgactc gtcttcggcg atcgtatggc atcgttgccc gtctcgagcc
     5521 tcaagtcgat gatcggccat ccccagggcg cctgtggtgc ggccggcgtc gtcgcctcgc
     5581 tgctcgcgat gcgcgatcgt ttcattccgc cgacgatcaa ctacgatctc ttcgatccgc
     5641 agtgcgatct ggacgtcgtc ccgcacgagg gacgccccgg cgagctcggc gcggtgctgt
     5701 gcaactgcat cggcttcggc tcgaagaact cggcgttggt cttttgcgcg accgatcccg
     5761 gcggcgggtc gctggtcgac gtccgcagga cgtgaaccgt gcgactggtg cccgaccgcc
     5821 gcgacgatgc ggaatggatc gatcgccccg acaacacggt cgccgatctc cgcgcggcct
     5881 tcggcgacat cggttggacc catcgttggc tcggcgggcg ggcggtcctg ctgtcggcgc
     5941 tcggcccgta cctcgaccgg tcggatggac cgctccggat cctcgacgtc ggtacgggcg
     6001 ctgccgatct gccgttggcg atcatcgcct gggcgcgacg tctcggccgc ccggtggagg
     6061 tcgtcgggct cgaccgcgac ttcggggtga cgtcgatcgc cgccggcgcc gcggccggtt
     6121 gcgccgaact gacggtcgtc tgtgccgacg cgcggcgcct gccgttcgac gatcgatcgt
     6181 tcgatctggt caccgcgtcg ttgttcctgc atcacttcgg gccgggcgag tggatcaagc
     6241 tgctcggcgg cttccgtcgc gtggcgcgcc gcgccgtcgt cgtctgtgac ctcagacgtc
     6301 atctgctccc gtgggtcttc ctcgccggcc tcgcccgact gacccggcgc cacccgatgt
     6361 tcgtccatga tgctccgctg tcggtgctgc gcggattcac cgatcgcgag ttgctcgatg
     6421 cggccaccgc atgcggggcg tcctcggtca gcctgcgccg gcgctggccg ttccgcctgc
     6481 tgctgacgat ccgcgtcgag ggagaggcgg cgtgagacgc cgctcggacc tgctcgtgct
     6541 cggcggtgga ccggccggct ccaccgtggc ggcctgggcg gcagccgccg gttgcgcggt
     6601 gacggtcgtc gatcaggcgc ggtttccgcg ggacaaggtc tgcggcgagt tcctgtcgtc
     6661 cgagggttgc cgggtgctcg aacggatcgg gctgctcggc ggactgatcg cacagggtgc
     6721 cgttccgatc aaccgcttcc gcatcggcga cgcgtcgggg cgctcgatcg aggcaccgct
     6781 cccggcgcca ggcgatccga taccgcgcgc gctcggtgtc tcgcgcgcgc ttctcgaccg
     6841 gctcctgctc gagcacgccg cggcccgggg cgcgcgcgtg ctcgagcgct gccgtgcgtt
     6901 gcgaccgatc gtcgagggcg gccgggtcga gggtgccgtc gtgcgcgcga tcgacggccg
     6961 gtcgagcgcg atcgagctcc gcgccacgct cgtcgtcgcg gccgacggcc ggcgctcgat
     7021 gctggctcga gcgctgcacc ccgattccgg cgacccgagc acgacacaca ccggttcgtg
     7081 gttcgggctg aagactcacg tcggcggcgc gcccggggag ctcgcggacc gtgtcgagct
     7141 ccagctgttc gacgggggct acgtcggact ggcggccgtc gacggaggcc ggatcaacgt
     7201 ctgcctgatg acgcgggtcg cggcgctccg tgcctgcggc gggtcgccgg atcggctgct
     7261 gtccgagcgg atcctggcca atccggcggt ggcgcgagtc ttgggcggaa gcgagcgctg
     7321 cggacggtgg cacgcgatcg gcccactcgc ctggggtccg cgtcgcccgg cggccgccgg
     7381 ggcactgttc gtcggcgatg ccgcgggcac gatcgacccg ttcagcggtt tgggtatctc
     7441 gaatgcactg cgcgcggccg agctcgtgag cgacacggtg ttggtcgcgg tcgatcgcgg
     7501 cgcgctggaa ccgggcgcgg cgcgtgcgta cgcccgccgc tggcgcgcga gcttcgcgcc
     7561 actgacgcgg cgcgtccgcg tggtcggcag cctgttcgag cgaccgcgac tggcggtgcc
     7621 gctgatcggc tggctgcgcg gagcgggttc cggctggttg ccccgcctgt tggccgccac
     7681 gcgcgccgag gtctgactcg tgaaacgcgg tgggtggctg gtcgccggtg cgttgctcgc
     7741 gggggccctg ttctgctcct cgctcggcgt gctgtggccg ctcgcaccga tcgacctcgt
     7801 cgtgccggag cggcagctcg tccgacaggc gctgggcttc ctcgagcaac gggggttcct
     7861 cctcggcggc tacgacagcg ccgagtggct acgcgtcgac gctccggggc tggactacgt
     7921 cgagcgagcg ttcggcaaag cccgggccga cgagtggatt cgggccggtc tcccgctgta
     7981 cgagtaccgc gttgcattca agcgcgccgg cgacccgctg gtcgtacggg tctcgctcca
     8041 tcccgaccgc ggtgccctcg gctggaccgc cgagctcccg cgggatctcg cgggcgagcg
     8101 gctcgatgcg gtgagcgcgc gtacgatcgc gcagcgagag atcgagccgg cgctcggtct
     8161 cgatctcgac caatggcagg aaacgtcgtt ctcgaccgaa ctgctgccgg cgagggtcga
     8221 ccaccggctg gtctacgagc gccagctcgt cgaacacccg gagctgcgcg aacggctctc
     8281 ggtcgtcgtc gcaggagacc gggtcgcgac cgccgtccga tcgatcgtcg tcccgccgtc
     8341 cgccgaacgc caagctcgac gcggcgaggc ggcttcggtc gcgctcgagt ctttcggttt
     8401 cctgctgctc ggtatcgccg cagcggcggc gttcgtcgtc ttcctgcgcc gcatctccgc
     8461 cggcgaggtt cgcctcggcc gtgccgcggg ttggcctgtc gttgttttcg tctgcttcac
     8521 cgtggcctcg gtgctcgatt cgtttcggct gttcctcggc tgggatccac tgtggccgcg
     8581 cgcggtgtcg gccttccgtg acctgctgct cgacagcctg acccaagtct gggtgctgtt
     8641 ggtgctgctg gccgtcgtcg ccgccggtga cgcgctcgat cgggcgagcg gcgcgaaccg
     8701 tggcaggtcg ctgtggctgt tggctcgcgg ccgtgtcgcg gatcgttcgg tcgcactggc
     8761 atcggggcgg gggttcctga tcggcctcgc ttgcggcgga gtgatggcgt cgatcgtcgt
     8821 gctgctgcat cagatcggag tcgctcgcag ctcgatccag ccacgcggtt tctttttcta
     8881 cccgctgaac agcgaggcac cggcgattac gacgctgctg tttttcttcg gcgtgtcgct
     8941 cgcggaagag ctcggttatc ggttcttcgg cggcacctgg ctcgaaggct tgacacgccg
     9001 gcgctggctc gccgtcgtcg ttcccgcgct ggtttacggg ttgacgcaca ccggcctcga
     9061 cttcctcccg ccggccgaac cgttctgggc gcggccgttg gtgctgaccg ccgtcggctg
     9121 tgtctggggc tgggccttcc tgcgctacga cgcgctgacc gtggtgctgt cccacttcac
     9181 cgccgatctg ttcatcttca attggccgag gctggcgagc ggcgaaccga cggcggtcac
     9241 cttggcgctg gtcacgatcg gggttccgct gctgcccgcc ctggcctggc cctggatcgg
     9301 ttcgcggaag ggctagtccg aagttcccgg ggcgattggg gctgatgctg gtgcaagtgg
     9361 ttgaaggagt gcgggttgga gagagcgcgt ccggatgaaa tgtagccact aatatggctt
     9421 gacacttccg ggcatgctgc ggcagaatag cgaccatg
//
LOCUS       DASPGN010000454         5562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1488779, whole genome shotgun sequence.
ACCESSION   DASPGN010000454 DASPGN010000000
VERSION     DASPGN010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488779"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VD788_13280"
     CDS             <1..534
                     /locus_tag="VD788_13280"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXV77284.1"
                     /translation="VGAGTLRADDPPLHVRSKEMREYRRELGKPPELTRVVATRSLEL
                     GDAGGFFRDDGARRIVATVQSAPEQRLRSLSARAEIWRIGAEWIDLGALLERLCREGV
                     ERLLVEGGGELNWSLLRDDLIDEVFVTVAPALLGGREAPTPIEGVGLAMSDQRRLKLI
                     EVERSGDELFTRWAVRR"
     gene            534..1181
                     /gene="nth"
                     /locus_tag="VD788_13285"
     CDS             534..1181
                     /gene="nth"
                     /locus_tag="VD788_13285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXV77285.1"
                     /translation="MGGRTDPIRHRARRIVERLRSGYRDADTALHHRSALELLVATIL
                     SAQCTDERVNRVTPVLFAAYPDAAAYALADRASLEERIRPTGFYRNKATALIGLGGAL
                     VRDHGGRVPDTLDALVALPGVGRKTANVVLGTWFGKPAIPVDTHVKRVSNRLGLTVEH
                     DPTKIELDLKRLLEDGDWTFASHALILHGRAVCHARKPACDRCVLRVDCPFPVGR"
     gene            1252..2127
                     /locus_tag="VD788_13290"
     CDS             1252..2127
                     /locus_tag="VD788_13290"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXV77286.1"
                     /translation="MAGKPRFETIDGDDLYRRLALGKSILVLDVRTPSEFETGHIPGS
                     MLIPLHELADRVAEVPNSGMPIALVCEHGLRSVSGCRLLAEHGMGPLFNLSGGLAAWP
                     GPLSAPATNGRQLHGIAPSSFLVDSFGLLPRGLALDLAMGEGRNAIYLATRGYDVDGV
                     DANPHLVERARAAARKLGAPIRAVVGDVEDGTYIIPIESYDLIVVFNYLHRPIMKDIK
                     DGVMPGGGVIYQTYTADQARFGKPTNPDHLLDPGELKQTFADWEILRYRELIGPARKD
                     GENRAIAGIVARKPA"
     gene            2191..2877
                     /locus_tag="VD788_13295"
     CDS             2191..2877
                     /locus_tag="VD788_13295"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXV77287.1"
                     /translation="MTSSLARDQRLPGVVPVFPLPNVVLFPGTIVPLHVFEPRYRQMI
                     VDSAAADRVIGIALLEPGYDADYQGHPPFHPIGTVGRIDQLDRLPDGRFNMNVVGLHR
                     VAYDEIPSSKPYRLTRLTRLPEKEVDDLCPEIVRAKRDLLVSQAMLLRELKGDDDPTL
                     IIDERLPFALAVNGACANLPVASEVRQELLAIDCLVERHRRASRLIAGVLQSLLSMRG
                     GELADDARPS"
     gene            complement(2881..3870)
                     /gene="moaA"
                     /locus_tag="VD788_13300"
     CDS             complement(2881..3870)
                     /gene="moaA"
                     /locus_tag="VD788_13300"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HXV77288.1"
                     /translation="MLEDGFGRVHDDLRISVTDRCNLRCAYCMPVDPVWFPRSELLSF
                     EEILRLARILAARGVRKVRLTGGEPLVRRDLPELVAMLADEPRIEDLSLTTNGLLLER
                     AAAELARAGLSRVNVSLDTLLPHRFHAMTRRDGLAKVLAGIDAASRAGLGPIKVNTVV
                     LRGHNEDEVEGLVERARAQAWELRFIEFMPLDNDHGWRVDRVVTGAELVERIGARWPL
                     DPDPQADPHAPARRFLFRDGGGAIGFIDSVSRPFCADCSRLRLTSDGYFRVCLYDRDE
                     VDLKTPLRGGADDGELERLMVRALARKGRGGALDILESRRVLPLRRTMHQIGG"
     gene            3946..4764
                     /locus_tag="VD788_13305"
     CDS             3946..4764
                     /locus_tag="VD788_13305"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HXV77289.1"
                     /translation="MRTRAKKAYKNLEFLISREARTIRILSEYLEPRARFAHYNITDT
                     VVFFGSARALAPDVAQGQLREAETSGDAQRLAGARQAVRLSRYHDDATTLARLLTEWS
                     KSLQLPERRFIVCSGGGPGMMEAANKGASQASGLSIGLGISLPSEPSSNHYITRELNF
                     EFHYFFMRKFWFVYLAKAMVIFPGGFGTMDELFELLTLIQTRKTEKRLPIVLYGKEFW
                     DEVLNLEALVRWGTISAEDLELFEVFSTPQSAFDHLRRELTRLYLEPKAAEAGS"
     gene            4761..>5562
                     /locus_tag="VD788_13310"
     CDS             4761..>5562
                     /locus_tag="VD788_13310"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HXV77290.1"
                     /translation="MNGYRRPSWLRVVVAALCVAALVWTIAALGPVRVIEAAAGVDPG
                     WIALALVAIAARFAIWAVKWMLILRQRERVSYRLCLGLVLAGAFVNLATPTAKLGGGI
                     VRSLLLSRLRGWPKLESFGWSLADQATNLLGQWALFGVVAVAAAPSAGDPLVATLLTG
                     GGVGSLLVVALAGLARDRLLAAGRLAGLGRLAGRLIPTRFRGERVVAGGDGLAALVRP
                     IRAGRLATWYGRDVVLGGLGFASLCVSNLLVYRALGAEVPWLLGSAAVL"
BASE COUNT          818 a         1871 c         1951 g          922 t
ORIGIN      
        1 gtcggcgccg gcacgctgcg ggccgacgat ccaccgcttc acgtgcgctc gaaggagatg
       61 cgcgaatacc gccgcgagct gggcaagccg cccgaactga cccgcgtcgt cgcgacacgc
      121 tcgctcgagc tgggcgatgc cggtggcttc ttccgcgacg acggcgccag acggatcgtc
      181 gccacggtcc aatcggcccc cgagcaacgg ctgcgcagcc tgtccgcacg agccgagatc
      241 tggcgcatcg gcgccgagtg gatcgatctc ggggcgctgc tcgagcgctt gtgccgcgag
      301 ggggtcgagc ggttgctcgt cgagggcggc ggcgagctca actggtcgtt gctgcgcgac
      361 gacctgatcg acgaggtgtt cgtgacggtg gccccggcgt tgctcggcgg tcgtgaggcg
      421 ccgacgccga tcgagggcgt gggcctcgcg atgtccgacc aacgacgact gaagctgatc
      481 gaggtcgagc ggtccggcga cgagctgttc acccgctggg ccgtgcgccg ttgatgggcg
      541 gacggacgga tccgatccgg catcgcgccc ggcgcatcgt cgaacgcctg cgcagcggct
      601 atcgcgacgc cgacacggcg ctccaccacc gatcggcgct ggaactgctg gtggcgacga
      661 tcctgtcggc ccagtgcacg gacgaacggg tgaaccgcgt gacccctgtg ttgttcgcgg
      721 cctaccccga cgccgcggcc tacgccttgg ccgatcgggc gtcgttggag gaacggatcc
      781 gcccgaccgg cttctatcgg aacaaggcga cggcgctgat cggtttggga ggtgccctgg
      841 tgcgcgatca cggcgggcgg gtaccggata cgctggacgc gttggtcgcg ctgccgggcg
      901 tcgggcgcaa aacggcgaac gtggtgctcg gaacgtggtt cggcaagccg gcgattccgg
      961 tcgacacgca cgtcaagcgc gtgtccaacc ggctcggtct gaccgtggag cacgacccga
     1021 ccaagatcga gctcgaccta aagcgactgc tggaggacgg cgattggacc ttcgcctcgc
     1081 acgcgctgat cctgcacggt cgggcggtgt gccacgcccg caaacccgcg tgcgatcgct
     1141 gtgttttgcg tgtcgactgc ccgttccctg tcgggcgcta gccgcgggtg ggcgccttgc
     1201 cgtcgaggcg ctatattgac accgcttggc gagggtgcgg gagtacttcc ggtggctggc
     1261 aagccgcgat tcgagacaat cgatggcgac gatctgtacc gtcgactggc cttgggcaag
     1321 tcgatcctgg tgctggacgt ccgcacgccg tccgagttcg aaacgggtca catccccggc
     1381 tcgatgctga tcccgctgca cgagctggcc gatcgcgtcg ccgaagtccc caacagcggg
     1441 atgccgatcg cgctggtctg cgagcacggc ttgcgcagcg tgtcgggctg ccggctgctc
     1501 gccgagcacg ggatgggccc gctgttcaat ctgagcggcg gcctcgccgc ctggcccggg
     1561 ccgctgtcgg cgccggcgac gaacggccgg caactgcacg gcatcgcgcc gtcgtcgttt
     1621 ctcgtcgaca gcttcggcct gctgcccagg ggtctcgctc tcgatctggc gatgggcgag
     1681 ggccgaaacg cgatctatct cgccacccgc ggttacgacg tcgacggcgt cgacgccaac
     1741 ccgcacctcg tcgaacgggc gcgcgccgcg gcgcgcaagc tgggggcacc gatccgcgcg
     1801 gtggtcggcg acgtcgagga cgggacctac atcatcccga tcgagagcta cgacctgatc
     1861 gtcgtcttca attacctgca ccgaccgatc atgaaggaca tcaaagacgg cgtgatgccc
     1921 ggcggcggcg tgatctacca aacgtacacc gccgatcagg cgcgcttcgg caaaccgacg
     1981 aaccccgacc acctgctcga tcccggcgag ctcaagcaga ccttcgccga ctgggagatt
     2041 ctcaggtacc gcgaattgat cgggccggcg cgcaaggacg gcgagaaccg cgcgatcgcg
     2101 ggaatcgtcg cgcgcaagcc ggcctaggcc ggtttgtcgg gtgtgctcgt tcgctctatg
     2161 ctgagtcggg ggtacgggag gcccggcaca gtgacgagca gcctcgcgcg agatcagcgg
     2221 ttaccggggg tcgttcccgt cttcccgctg ccgaacgtcg tgttgtttcc gggaacgatc
     2281 gtgccgctgc acgtgttcga gccgcgctac cggcagatga tcgtcgacag cgccgcggcg
     2341 gaccgcgtaa tcggtatcgc gttgctcgag ccgggttacg acgcggacta ccagggacac
     2401 ccgccgtttc acccgatcgg aaccgtcggg cggatcgacc agctcgaccg gctgcccgac
     2461 ggtcgtttca acatgaacgt ggtcggcctg caccgtgtcg cctacgacga gatcccgtcg
     2521 tcgaagccct accggctgac gcgattgacg cggctgccgg aaaaggaggt cgatgacctc
     2581 tgccccgaaa tcgtgcgcgc caagcgcgac ctgctcgtca gtcaggcgat gctgctgcgc
     2641 gagctgaagg gcgacgacga cccgacgttg atcatcgacg agcggttgcc gttcgcgctg
     2701 gccgtcaacg gcgcatgcgc caacctgccg gtcgcgtcgg aggttcggca ggaactgctc
     2761 gcgatcgact gtctggtcga gcgtcaccgc cgggcctcgc ggctgatcgc cggggtattg
     2821 caaagcctgc tcagcatgcg cggcggcgag ctcgccgacg acgcccgccc gtcgtgacgc
     2881 tcagccgccg atctggtgca tcgtgcgcct gagcgggagc acccgtcgac tctccaggat
     2941 gtcgagcgct ccaccgcgtc ccttgcgcgc cagcgctcgg accatcagcc gctcgagctc
     3001 gccgtcgtcg gcgccacccc gcagcggggt cttcagatcg acctcgtcgc ggtcgtagag
     3061 acagacgcgg aaatagccgt cggaggtcag ccggaggcgg ctgcagtccg cgcagaacgg
     3121 ccggctgacc gagtcgatga agccgatcgc gccgccgccg tcgcggaaca ggaaccgccg
     3181 cgcgggggca tgggggtcgg cctgtgggtc gggatcgagc ggccagcgcg ccccgatgcg
     3241 ctcgaccagc tcggccccgg tcaccacccg atcgactcgc caaccgtgat cgttgtcgag
     3301 cggcatgaac tcgatgaacc gtagctccca cgcttgggcg cgggcccgct cgaccagccc
     3361 ctcgacctcg tcctcgttgt gaccgcgcaa gacgacggtg ttgaccttga tcggtccgag
     3421 cccggcacgt gaggctgcgt cgatcccggc gagcaccttg gccaagccgt cgcggcgcgt
     3481 catcgcgtgg aagcgatgcg gcagaagcgt gtccaggctg acgttgaccc gcgagagacc
     3541 tgctcgagcc agttcggcgg cggcccgctc gagcaacagg ccgttggtgg tcagcgacag
     3601 atcctcgatg cgcggctcgt cggccagcat cgcgacgagc tcgggcaggt cgcgccgcac
     3661 caacggctcg ccgccggtca gccgtacctt gcgcacgccg cgcgcggcca ggatgcgggc
     3721 gaggcgcaga atctcctcga agctgagcag ctccgaccgc gggaaccaca ccgggtcgac
     3781 cggcatgcag taggcgcaac gcaggttgca gcggtcggtg accgagattc gcagatcgtc
     3841 gtgtactctg ccgaagccgt cttcgagcat cgaccggcct ccacttgcgg gtccgagcga
     3901 cccttgtata ctcgcatact tctgccgagc cgtcggaagg tgacgatgcg gacccgtgcc
     3961 aagaaggcct acaagaacct ggagttcctc atcagtcgcg aggcgcggac gatccgcatc
     4021 ctgtcggaat acctcgagcc gcgggcgcgg ttcgcccact ataacatcac ggacaccgtc
     4081 gttttcttcg gctcggcgcg ggcgctggcc ccggacgtcg cacaagggca gctgcgcgag
     4141 gccgagacga gcggcgacgc ccagcggctg gcgggcgcgc gacaggcggt ccgcttgtcg
     4201 cgctaccacg acgacgcgac gacgctcgca cgcttgctca ccgagtggtc gaagtcgttg
     4261 cagctaccgg agcggcgctt catcgtttgt tcgggcggcg gtccgggcat gatggaggcg
     4321 gcgaacaagg gcgcctcgca agcctcggga ctgtcgatcg gactggggat cagcctaccg
     4381 agcgagccgt catcgaatca ctacatcacg cgtgagctga acttcgagtt ccactatttc
     4441 ttcatgcgca agttctggtt cgtctatctg gccaaggcga tggtcatttt cccaggcggg
     4501 ttcggcacga tggacgagct gttcgagctg ctcacgctga tccaaacgcg caagaccgag
     4561 aagcggctgc cgatcgtgct ttacggcaag gagttctggg acgaggtgct gaacctcgaa
     4621 gcgctggtgc gctggggcac gatctcggcc gaggatctcg aactgttcga ggtcttctcc
     4681 actccgcaat cggcgttcga tcacctgcgg cgcgagctga cccgtctcta tctcgaaccg
     4741 aaggcggcgg aagccggctc ttgaacggct accgccggcc gtcgtggctg cgtgtcgtgg
     4801 tagccgcact gtgcgttgcg gcgctggtgt ggacgatcgc cgctctcggg ccggtccgcg
     4861 tgatcgaggc cgccgccggc gtcgacccgg gctggatcgc tctcgcgctg gtcgcgatcg
     4921 cggcgaggtt cgcgatctgg gccgtcaagt ggatgctgat cctgcgccag cgggaacgcg
     4981 tttcctaccg gctgtgcctc ggactggtgc tcgccggcgc gttcgtcaat ctggcgacgc
     5041 cgacggccaa gctgggcggc gggatcgtga ggtcgctgtt gctgtcgcgc ctgcgcggtt
     5101 ggccgaagct cgagtccttc ggctggtccc tggccgacca ggcgaccaac ctgctcggcc
     5161 agtgggcgct gttcggcgtc gtggcggtgg ccgcggcacc ctcggccggc gatccactcg
     5221 tggcgacctt gctgaccggg ggcggtgtcg ggtcgctgct cgtcgtcgct ttggcgggtc
     5281 tggcgcgcga ccggctgctc gccgccggtc gtcttgccgg gctgggccgg ctcgccggcc
     5341 ggttgatccc gactcgcttc cgcggcgaac gggtcgtcgc gggcggcgac gggcttgccg
     5401 cgctggtccg gccgatccga gcaggccggc tcgcgacctg gtacggtcgc gacgtcgtgc
     5461 tcggtggact gggcttcgcc tcgctctgcg tgtcgaacct gctggtctac cgcgcgctcg
     5521 gcgcggaggt cccgtggctg ctcggctcgg ccgccgtact cc
//
LOCUS       DASPGN010000455         3873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1137972, whole genome shotgun sequence.
ACCESSION   DASPGN010000455 DASPGN010000000
VERSION     DASPGN010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137972"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VD788_13315"
     CDS             <1..1264
                     /locus_tag="VD788_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77291.1"
                     /translation="FRRYFELRRDRAVYKEQPDNWFISARQWSRSADIEEQEHTLSGL
                     HADYIFFMVDEVGGVPEAVLAAAEGALATGIECHLVVAGNPTHCDGPLWRACRQDRDL
                     WTLIEVTGDPSDPKRAPRVDRDWAIHQIQKYGRRSPFVLVNVFGKFPVTSSEALVSVE
                     DVLNAFDRNFAYLETPLGYRRDSRGNALERGTRVAGLDVSRRGSALNVFTVRDGDYVL
                     EPISWQGQDHVWTAGRAVDLMRELDVERLYVDDVGYGGGVTDLLVRQGVQVVPVNVGI
                     PAADDEHYVNLRAEFAGRLQERFRTGRISLDPSFRDTEFVTEATTLSVDFDKRNRKIV
                     EDKDRYRQRVGRSPDFWDSLALTMYEEVQPPGQGACGEERELFRGRNYVAIHRGGEVR
                     VVPRGTEDEDDPAVNEWVPRRRAMRLRA"
     gene            1261..2850
                     /locus_tag="VD788_13320"
     CDS             1261..2850
                     /locus_tag="VD788_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77292.1"
                     /translation="MNLRDTLRRMTGPDPRIDELSSGLTEALDGLKAAKAEIGRLEGL
                     LDVVNDPDSQTIGAGNASLFREIWGGHTASRDFDENQRRKILGLSHLVYALRGEAENL
                     IDTQLDFILGDELMPVSQHEALLKRLKEVWEDERNCLGEKHEAHTLAWMLDGELHLRA
                     YVSAEDGHVELGWLDPLAVNRVVQDRRGRDAFLHVAPEKPGGANQVLFCLQSLNERVT
                     LEPLAAGRWRIEDEAGQARTVDGLAYSGFWARPPGATRGRPDLSQVIDEIDASNELVW
                     STVEVQNLRRWFLLWVKDPSIRTKAEATETLRKLNLVSPPRNPRSIATNSQVEIDLLA
                     NQQTGAGDEWLARELSAKIGGAKGLPGAWFGRHADQRESGARASDLVPLRRLRRKQRR
                     VTRFWHGVVASMLDVQARVTGDQVPEGTWEMQALEVGGRDRQRGAEIIKAIAAAAVQA
                     QSSGTAKRELLHAVLVQSFREAGFEVQREHEGVPEDELEQQVAGARAMVNQLVTRKSR
                     TGEGGQDEEDRDRVRERTDAA"
     gene            2840..3709
                     /locus_tag="VD788_13325"
     CDS             2840..3709
                     /locus_tag="VD788_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77293.1"
                     /translation="MPRDPEAIVRAYSRKIDRLIRRGERLTEDDARRLRRLFVAFRAQ
                     ALARLPVVSQLNRAAIAGVIGALDAQLQVLADQVHDLVEAGTAAQLDLAREMTRVYGE
                     TFVDELDLDAVMRQFSGARLSALDAVSGFSAELIGVSSGGLRADILSEVNREIRLSAL
                     GAGEGTFAGTVAVERALSGVAKWTARAERIYRTETLRAHSIVTEQTIRELNRYSPTRK
                     RWNWSGISRREHAAIDGQTIEVGGRYRVPLRDGGTVLMRWPRDPSAPASATINCGCYE
                     TPVPAESTARRAA"
BASE COUNT          661 a         1197 c         1431 g          584 t
ORIGIN      
        1 cttccgcagg tacttcgagc tgcggcgcga ccgcgcggtg tacaaggagc agccggacaa
       61 ctggttcatt tcggcgcgtc agtggtcgcg atcggccgac atcgaagagc aagagcacac
      121 gctttcgggc ttgcacgccg actacatatt cttcatggtc gacgaggtcg gcggggtgcc
      181 ggaggccgtg ctggccgcgg cggagggtgc gcttgcgacg ggcatcgagt gtcacctggt
      241 ggtggccggg aacccgacgc actgcgacgg cccgctgtgg cgcgcgtgcc ggcaggaccg
      301 cgacctgtgg acactgatcg aggtcacggg tgacccttcg gacccgaagc gcgctccgcg
      361 cgtcgatcgc gactgggcga tccaccagat ccagaagtac ggccggcgct cgccgttcgt
      421 gctggtgaac gtgttcggca agttcccggt gacgagctcg gaggcgttgg tctcggtcga
      481 ggacgtactg aacgccttcg accgcaactt cgcctacctc gagacgccac tcggttaccg
      541 acgcgactcg cgcgggaatg cgctcgagcg cgggacgcgc gttgcggggc tcgacgtgtc
      601 gcgtcgcggg tcggcgttga acgtgttcac ggttcgcgat ggggactacg tgctcgagcc
      661 gatctcgtgg caggggcagg accacgtatg gacggccggg cgcgcggtcg acctgatgcg
      721 cgagctcgac gttgagcggc tgtatgtcga cgacgtgggc tacggcggag gcgtgaccga
      781 cttgctggta cgccagggcg tgcaggtcgt gccggtgaac gtgggtatcc cggccgcgga
      841 cgacgagcac tacgtgaatc tccgcgcaga gttcgccggc aggctgcagg agcgtttccg
      901 cacgggtcgc atctcactcg atccgtcgtt ccgcgacacg gagttcgtga ccgaggcgac
      961 gacgctgtcg gtcgacttcg acaaacgcaa ccggaagatc gtcgaggaca aagaccggta
     1021 ccgccagcgc gtcgggcgct ctcccgactt ttgggattcg ctggcactca cgatgtacga
     1081 agaggtccag ccgccgggtc aaggcgcgtg cggcgaggaa cgcgaactct tccgcggcag
     1141 gaactacgtc gcgatccacc gcgggggcga ggtgcgcgtc gtgccgcgtg gtaccgaaga
     1201 cgaagacgat ccggcggtca acgagtgggt gccgcgccgg cgcgcgatga ggctgcgggc
     1261 atgaacctgc gcgacacgct ccgccggatg accggtcccg acccgcggat agacgagctg
     1321 tcgtcgggtc tgaccgaagc gctcgacgga ttgaaggccg cgaaagcaga aatcggtcgg
     1381 ctcgaggggt tgctcgacgt cgtcaacgac ccggattcgc agacgatcgg tgcggggaat
     1441 gcctcgctgt tccgcgagat ctggggcggg cacacggcgt cgcgcgactt cgacgagaac
     1501 cagcgccgca agatcctcgg gctgtcgcat ctcgtctatg cgttgcgcgg cgaggccgaa
     1561 aatctgatcg acacgcagct cgatttcatc ttgggcgatg agctgatgcc ggtgtcgcag
     1621 cacgaggcgc tgctcaagcg gctgaaggaa gtctgggagg acgagcgcaa ctgcctcggc
     1681 gagaaacacg aagcgcacac gctcgcctgg atgctcgacg gggagttgca tctgcgggcc
     1741 tacgtgtcgg ccgaggacgg ccacgtagag ctcgggtggc tcgacccgct ggccgtgaac
     1801 cgcgtggtac aggaccgacg cggacgcgat gcgttcttgc acgtcgcacc cgagaagccg
     1861 ggcggcgcga atcaagtgct gttctgcctg cagtcgctca acgagcgagt gacgctcgaa
     1921 ccgctggcag ccggccgatg gcgcatcgaa gacgaggcgg gtcaagcgcg caccgtcgat
     1981 gggcttgcgt actcgggatt ctgggcgcgc ccgccaggcg cgacacgcgg ccggccggat
     2041 ctatctcagg tgatcgacga gatcgacgca tcgaacgagc tcgtatggtc aacggtcgag
     2101 gtgcagaacc tgcgccggtg gttcctgctg tgggtgaaag acccgtccat tcggacgaag
     2161 gccgaggcga ccgaaacgct ccgcaagctg aacctcgttt cgcctccgcg gaatccacgg
     2221 tcgatcgcga cgaacagcca agtggaaatc gacttgctcg cgaaccagca gaccggcgcg
     2281 ggcgatgagt ggctcgcgcg cgagctgtcc gcgaagatcg gcggagccaa gggcctgccc
     2341 ggcgcatggt tcgggcgcca cgcggaccag cgcgaatcgg gtgcgcgggc gtcggatctc
     2401 gtgccgcttc gccggctgcg ccgcaagcaa cggcgagtga cgcgcttctg gcacggcgtc
     2461 gttgcgtcga tgctcgacgt gcaggcacgc gtgacgggcg accaggtgcc agagggcacg
     2521 tgggaaatgc aagcgctcga agtgggtggg cgcgaccgcc aacgtggtgc ggagatcatc
     2581 aaggcgatcg cggcggctgc cgtgcaggca cagagctccg gcaccgcgaa acgcgaattg
     2641 ctgcacgccg ttctcgtcca atcgttccgc gaagccggct ttgaggttca gcgcgagcac
     2701 gagggcgtgc cggaggacga gctcgagcag caggtcgcgg gcgcccgcgc gatggtcaac
     2761 cagctggtga cgcggaagtc gcgtacgggc gaaggcggcc aggacgaaga agaccgcgac
     2821 cgggtgcggg agcgtaccga tgccgcgtga cccggaagcg atcgtgcgcg cctactcgcg
     2881 aaagatcgat cggctgattc gccgcggcga gcggctgacg gaagacgacg cgcgccggct
     2941 gcggcggttg ttcgtcgcgt tccgcgccca ggcgttggcg cgcctgcccg tcgtatcgca
     3001 gctgaaccga gccgcgatcg caggggtcat cggggcactc gacgcgcaac tacaggtgct
     3061 tgcggaccag gtgcacgatc tggtggaggc cggcaccgcg gcacaactcg acctggcacg
     3121 cgagatgacg cgggtgtacg gcgagacgtt cgtcgacgag ctcgacctcg acgcggtgat
     3181 gcgccagttc tcgggagcgc gcctgtcggc gctcgacgcg gtgtccgggt tttccgcaga
     3241 gctgatcggt gtgtcgagcg gcgggctgcg cgccgacatc ctgagcgaag tgaatcggga
     3301 gattcgcctg tcggccctcg gggccggcga ggggacgttc gccggaaccg tcgccgtcga
     3361 gcgcgcgctg tcgggcgtcg cgaaatggac cgcgcgagcc gagcggatct accgcacgga
     3421 gacgctgcgg gcgcattcca tcgtgacgga gcagaccatt cgcgagctga accgctactc
     3481 cccgacgcgc aagcgctgga actggagcgg catctcgcgc cgcgaacacg cggcaatcga
     3541 cggacaaacg atcgaagtcg gcgggcgcta ccgggtgccg ctgcgagacg gggggacggt
     3601 gctgatgcgc tggccgcgcg acccgtcggc tccggcttcc gcgaccatca attgcggctg
     3661 ttacgagacg cccgtgccag cggaatcgac cgcgcgccgc gcggcatagg aggtggggat
     3721 gcccctgtac atcgacacgc agtaccggcg gcaggtggtc gggttgcaag agcggctgat
     3781 cgaggtcgag gtacgcgctg tcgacgagac ccgcgaggca atgaccgcgg ccgtcgaagc
     3841 gtgggttgcc gggaagctcg agccggtcaa tgt
//
LOCUS       DASPGN010000456         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_436577, whole genome shotgun sequence.
ACCESSION   DASPGN010000456 DASPGN010000000
VERSION     DASPGN010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436577"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VD788_13330"
     CDS             <1..1185
                     /locus_tag="VD788_13330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77294.1"
                     /translation="PCDDFEIDAACRAEIAEYLDKRNSDSLRGDYTETGSTAFFQHAS
                     TLYHPTAGCLSDAEIALQQQLDPENGPRCNFVPSASGALIARDYDAEFLAGTAQIFKN
                     ELAAVSWNFAMGLVVTSACTSDEGRGSDPECFDPEHPWVLDKCSFNQPQFCKKIKEFF
                     ELGGVQRNTLRAAGNPRFGRRTFLWHSGSEIVLTYDRRNVVGFAMDFPEDLSKTSWGV
                     EFTWFDSIPVYDASEFDSLSYVDTFNLTISIDRPTFIHALNPNRTFFFNTQWFFRYLA
                     NYSSGMGPGGAFGVLFTNTVSTGYFQDRLNPSLVTIYDFDSRSGGVLPALQYRISDSL
                     SAAVGLGIFFGRTQLMEMGINPFRFATNRAGPNAYKDSTDPFLSSVRSRDEIWLRLRW
                     TF"
     gene            complement(1251..>2182)
                     /locus_tag="VD788_13335"
     CDS             complement(1251..>2182)
                     /locus_tag="VD788_13335"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HXV77295.1"
                     /translation="TQTYTGEFFQLRTAHRADLAESAFALPGSHEQIWIGGGRFDAPL
                     DARDLGWRQLRPRAADRDPTEPDLTFVYAPAMRKLRRSATAWVDGMFTPSFRATAAGG
                     GGALASGSNALAPNGALSPGATLAAAASEHLPTGFSDISLRPNAYRWRLVGEREVLAP
                     INGRQSGYPQNAARNFGSSGLSLGSDRWDVRYAVVVEGTPLRGDHGFEALTAYIDYQT
                     QLPLYLITRRGAGRIAEIAIPVHRFSGDVADYPDDPGAERALVFDPVAVAVYRAADGG
                     SGWRRESYDVRSTPPSRAELREMTSTDPLLRGR"
BASE COUNT          333 a          762 c          734 g          353 t
ORIGIN      
        1 ccctgcgacg atttcgagat cgacgccgcg tgccgcgccg agatcgccga gtatctcgac
       61 aagcgcaact cggacagcct gcgcggcgac tacacggaga ccggatcgac cgcgttcttc
      121 cagcacgcgt cgacgctgta ccacccgacc gcagggtgcc tcagcgacgc cgagatcgcg
      181 ctgcagcagc agctcgaccc cgagaacggg ccgcgctgca acttcgtgcc gagcgcatcc
      241 ggcgcgctga tcgcgcgcga ctacgacgcg gagttcctcg ccggcaccgc gcagatcttc
      301 aagaacgagc tcgcggcggt gtcgtggaac ttcgcgatgg gtctggtcgt gacctcggcg
      361 tgcacgagcg acgagggccg gggctccgac cccgagtgct tcgatcccga gcacccctgg
      421 gtgctcgaca agtgctcgtt caaccagccg cagttctgca agaagatcaa ggagttcttc
      481 gagctcggcg gcgtgcagcg caacacgctg cgcgcggcgg gcaaccctcg cttcgggcgc
      541 cgcaccttcc tctggcacag cggcagcgag atcgtgctga cctacgaccg gcgcaacgtg
      601 gtcggcttcg cgatggattt tcccgaggat ctcagcaaga cgagctgggg cgtcgagttc
      661 acctggttcg attcgattcc cgtctacgat gcgagcgagt tcgacagcct gagctacgtc
      721 gacaccttca acctcacgat ctcgatcgat cgacccacct tcatccacgc gctgaatccg
      781 aaccgcacct tcttcttcaa cacgcagtgg ttcttccggt acctcgcgaa ctactcgagc
      841 gggatggggc ccggcggcgc gttcggcgtg ctcttcacca acaccgtctc gaccggctat
      901 ttccaggacc gcctgaatcc gtcgctcgtg acgatctacg acttcgattc gcgctccggc
      961 ggcgtgctgc cggctctcca gtaccgcatc agcgactcgc tctcggcagc cgtcgggctc
     1021 gggatcttct tcgggcgcac gcagctgatg gagatgggga tcaatccgtt ccgcttcgcg
     1081 accaaccgcg cgggaccgaa cgcctacaag gacagcaccg acccgttcct ctcgagcgtc
     1141 cgctcgcgcg acgagatctg gctgcggctg cggtggacct tctagcccgg gcagtagcgt
     1201 tgctcatcga cggggtatta ccagccggga cccagctcac tgggtcggtg ttaccggccg
     1261 cggagcaggg ggtccgtgct ggtcatctcg cgcagctcgg ctctcgaggg cggggtcgat
     1321 cgcacgtcgt agctctcgcg gcgccagccg ctgccgccgt cggccgcgcg gtagacggcg
     1381 actgcgaccg ggtcgaagac cagcgcgcgc tcagcgcccg gatcgtcggg atagtccgcg
     1441 acgtcgccgc tgaagcgatg cacgggaatc gcgatctccg cgattcgccc ggcgccgcga
     1501 cgcgtgatca ggtagagcgg cagctgcgtc tggtagtcga tgtaggcggt gagcgcttcg
     1561 aagccgtgat cgccgcgcag cggcgtcccc tcgacgacga ccgcgtagcg cacgtcccag
     1621 cgatcgctgc cgagcgacaa gcctgagctt ccgaagttgc gcgccgcgtt ctgcgggtag
     1681 ccggactgcc ggccgttgat cggtgcgagc acctcgcgct cgccgaccag ccgccagcgg
     1741 taggcgttcg gtcgcagcga gatatcgctg aaccccgtcg gcaggtgctc gctcgctgct
     1801 gcggcgagcg ttgcgccggg gctgagcgcg ccgttcggcg cgagtgcgtt cgagcccgac
     1861 gcgagtgcgc cgccgccccc ggccgcggtc gcgcggaagc tcggcgtgaa catcccatcc
     1921 acccacgcgg tcgccgagcg gcgcagcttc cgcatcgcgg gcgcgtagac gaaggtgaga
     1981 tcgggctccg tcgggtcgcg atccgcggcg cgcgggcgca gctggcgcca gccgagatcc
     2041 cgcgcgtcga gcggggcgtc gaaacgcccg ccgccgatcc agatctgttc gtgcgagccc
     2101 ggcagcgcga acgcgctctc ggcgagatcc gcgcggtgcg cggtgcgcag ctggaagaac
     2161 tcgccggtgt aggtctgcgt cg
//
LOCUS       DASPGN010000457        13696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_320427, whole genome shotgun sequence.
ACCESSION   DASPGN010000457 DASPGN010000000
VERSION     DASPGN010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 13696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13696
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320427"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VD788_13340"
     CDS             <1..932
                     /locus_tag="VD788_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV77296.1"
                     /translation="KDRPVVTVCRAGVVSRRAAGLLANLGFDAASLQGGIRGWGAVWS
                     EAPVRSLDPAKGSMTQVRRNGKGCLSYLLASRDEAVVVDPSVEPAAYRQLAEREGARI
                     THVLETHVHADHLSRARALCREVGARLLLPPNDRVDFSFTPFAHGERLRVGELVIEAV
                     ATPGHTGESTCLLVDDSILLTGDTLFLDGVGRPDLEAGDAGAEEGARALHASLHRRLL
                     DRFDDVLIYPAHHSGPILFDHAPIATRLSELRSKLPLLAAREDDFVAAILGRLQAKPP
                     NFDRVIEINEGKADLGAIDALDIEAGPNRCAAG"
     gene            complement(957..1439)
                     /locus_tag="VD788_13345"
     CDS             complement(957..1439)
                     /locus_tag="VD788_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77297.1"
                     /translation="MATSKPVRHFERGLVLLQNRRYAEAALQFQVAVQLERELGAVRP
                     QMRYRSFLGLSRTLAGRAKVEDVRLCEQAAKLDHFDPVLQLNLGKVYLRTGKRTRAIT
                     SFRLGLEMDPGNRELQQLFRRTDRRAAPVLPMLGRNHPVNVSLGRLRAKWTRRKAAAG
                     "
     gene            1852..3003
                     /locus_tag="VD788_13350"
     CDS             1852..3003
                     /locus_tag="VD788_13350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV77298.1"
                     /translation="MTKPMTKYYDDYQASGSLAPRPTRRESDPDSRGAAPPSAQLLPI
                     YLREMGQTPLLARDDEVRLARELQEARQAIARVMCKLPADCRRYVLDGDLGGPRAGYR
                     WPLDRLERACKRLSAYARDHAESSIEPLLDEVRRDKRRLDDSRDALIVANLRLVTHIV
                     KKYGNQGIPFMDLIQEGNIGLMKAVEKFEFERGYKFSTYAYWWIKQAISRAIADKARI
                     IRIPVHITEKVKRLKKVSNELKETLGRQPTSKEIARKMRLSVKKVDEILGVVQDPQPL
                     DTMGPEEEANGVLPFVADDKAVDPLERTLDHEITSKMRSALDVLDPREEEVIRMRYGI
                     GRKMRHTLEEIGIVLNLSRERVRQIEAIALRKIEATEESQSLRGHLFSK"
     gene            3088..4428
                     /locus_tag="VD788_13355"
     CDS             3088..4428
                     /locus_tag="VD788_13355"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV77299.1"
                     /translation="MSRIGRYLVASEVGRGAMGVVYLATDPRLKRQVAVKTMRWPDGL
                     TERQQSEFRERFLREAHAAAALSHAGIVTVYDAGEDAASAQPFIAMEYVEGCSLRQRL
                     DRDGPIEVGRAAEIAGAVADALHAAHERGIVHRDVKPANLLIRSADGAVKVTDFGVAR
                     VTNSELTQSGAGLGTPAYMSPEQIRGEPLDGRSDLFSLGVILYESLSGERPFDGETVP
                     SVIHSITLETPIPITKRTPGLPRGLDPFFDRALAKRPADRFPDGHAFRLALEAALGCR
                     DSPHTATVGALASGTSPAPATPLPPADRSLPWWRRRPRVAAAGALIAFLAVWLIAAWG
                     GDAYLHLEAKSSVDDGELTVLVDGTEVYRRWLDAPEQPKGLLKKMLEQSHETFEAYIE
                     IPPGKREVTAHVVHDGQSRPDRATIVLDIERGETRKLRLVAGRALGSSVSLKVQ"
     gene            4460..4876
                     /locus_tag="VD788_13360"
     CDS             4460..4876
                     /locus_tag="VD788_13360"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXV77300.1"
                     /translation="MMQYDDPPRSEGTVVDPRLAPRRLREGLVPEDTGIYLRVEEGAA
                     RGTVFTLSAGGVYVIGRDGADIAIPDEKVSRKHAEIGLYGPGAYVLRDLASTNGTLLN
                     GKRVQEKARLSNWDVIRIGDTVIRFQMVEGSIPLES"
     gene            4909..6471
                     /locus_tag="VD788_13365"
     CDS             4909..6471
                     /locus_tag="VD788_13365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HXV77301.1"
                     /translation="MDRDRLLERLERSDEPWDFIIVGGGATGLGALVDAASRGYRAVL
                     LEQHDFAKATSSRSTKLIHGGVRYLKEGNVSLVLEALRERGFLYRNAPHLVRNMAFVI
                     PVYSWWEGPFYGIGMKVYDRLAGRLGFNPSHTLTREETIERIPTVETDGLLRGVVYHD
                     GQFDDARLAIELARTAAELGGCPLNYMRVERLIDGGGLLRGVVARELEGGSVFEIEAR
                     AVINATGVFADELRRLDTPDCVPMLAPSQGTHLVLPKAFLPGDSAILVPRTDDGRVLF
                     AVPWHDHVVVGTTDTAVDRVSLEPRASAEEIEFLLTNAARYLSRDPGPRDVLSVFSGL
                     RPLVARGGAQGTANLSRDHSIVISNSGLVTITGGKWTTYRKMAEEVIDRAEIVADLDH
                     RPCRTETLPIHGHCTPSAAADALGVYGYEAAAIRALVESDPTLGRPLAPGVAVIGAQV
                     VWAVRREMARTVEDVLARRTRALLLGARASAEAADPTATLMARELGRDESWCRRTVQD
                     YRELARGYELSV"
     gene            6564..12326
                     /locus_tag="VD788_13370"
     CDS             6564..12326
                     /locus_tag="VD788_13370"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HXV77302.1"
                     /translation="MRVIAYTSIPLILLAAAALVGSSPHGAGAGEEARATTTVEPGLT
                     RAEALFAEGSYESALEIYRAIDLDALGDEERRWVRMRLADAAWRSAAATDGADEQRLE
                     QARQALEAEIRDRTREDEQDRVWAEVQESLGDYWWTRRGWPNWGQAWQHYDAALGWWA
                     GSSEIELARERYLSIVWRAARPPHVPPHYYYGYYGNQLPQSVLENARKIAVDPRDRAH
                     AAYLIAMGLRFHGGDPASVRRTARAFDEAVEAGRGTDWYDDALFHHAEWLAGSGRVVH
                     DERGEVTALPDFVRAVEQYRRLLREFRKGETRYYDQAEARVEQITAPEVGVVVSNVFL
                     PDAEVRYELGYRNVERVELALYPVELTRDLRFDTNDGSDTWLDGLAAVKGRPWHRWSH
                     DTGDAGEHVPGRAALMLEREPAPGAYLLEASAGGETARDLVLVSRVAIVQKVSAGRAL
                     VYVCDAIDGSPRGAAEVTLWVRREQSWERLAGRTDEQGLSAFELPAAGDRSETFAAAH
                     AGDDQAFSAGHAWHHRSESVPWRIYAFTDRPAYRPEDRVRFKVVARQVVDGGYRTPDG
                     RTLHYEIVDPRGTELARGTLELNAFGTAWAELAMVRTATLGEYRIDFREQEESGSIGS
                     AALFRLEEYKLPEFEVSVETPTADGRRLAFRVGDTVEADVAARYYFGGPVAGAEVEAV
                     IYQKPLWVWWEPPREFQWLYEDDARSRRQHWGGPGQIVERVRLTTDASGRATLRFDTP
                     AAGGQDFEYTIEARVRDASRREVEGRGTVRVSRQRYFAFVRAEHNLYRPGDEVRVELK
                     TIDPNGEPIGADGRVRVTREVWREIWSDPAGREVSGHELPPAERRGRGWTLTSSGYRP
                     VEIVDTAVRTGEDGSAEFRFEADEAGYYRVAFRGDDLADYPVRGETTVWVADSRTRTV
                     GYHHGGVEILVDRDTLRAGQSAPVMISAPVEGAWVLFSIEGEELYDYRIVHLDGSVKL
                     IEVEIEPRYVPNVFFEVALVHDGELWLDSRRVVIPPVEQFLDVAVEADRTEYGPGDTG
                     RLLLTTRDHEGQPVSAEVALALVDASVFYIEQDLAGDPRRFFFGDSRAKLVVTGSTFQ
                     QKPYVRRDGGHDDRSVDLGDELSRRYDVEGLSAREAAETDALEVVATPAPSPAVGAKM
                     RQEAEGAEISTVEVRTDFRATAVWQPEVVTDGNGAATVEVVFPDTLTAWRATARVTGR
                     DDRFGIAEAEMRTDKPLIVRLQAPRFFVAGDETTVSAVVNNRTDDDLEVTTSLDVTGA
                     VRDGAADRVLRVTAGAEARVDWRLRAEQAGQLELTARATAGELADGMRNRYPIHEHGI
                     DKRVYRAGKLRGEAVEIKLDLPAERRSTTMSVQVTPSLAITMLDALPYLIDYPYGCTE
                     QTMSRFLPTVIVADTLAELGLDRTQIARRLFGGIEPQHAARTHTGNERALERLDEMVR
                     AGLDRLYDFQHDDGGWGWWKVGDSDPYMSAYVVWGLSLANDAGVRVRSDVLERGVAFL
                     DRELVEAEERPDLQAWMLHALAVATERGGPGPFQRAALDNLWVRRDGLNAYTRALFAL
                     AAHELGQTERAETLVRNLANGVERDRSPDTSIVSRGAPHGSDAVIGTAHWGSDRFYWR
                     WSEGAIEATAFALRALLAIDPENELVEPATEWLIRNRRGAQWSNTRDTAFVVLALTDY
                     LTASGELSREAEYELFVNDRSIATRRVGPRDLFDAPSRFVVDPTAIEDGENRIRIVRR
                     SGDAALYFAAEATFFSLEEPVTPAGNQVFLRRQYHRLAARPTLLEGYATRLVPLDDFG
                     EVASGERVEVVLTIEVKNDYEYLVIEDLKPAGLEAVQLRSGEPIHARELAPSAAARPE
                     RSREPTDYTGRSRPMHQELRDRRVAFFIDDLPQGVWEVRYTLRAELPGRFHALPATIH
                     AMYVPEIRANGAELRLGVTD"
     gene            12377..13531
                     /locus_tag="VD788_13375"
     CDS             12377..13531
                     /locus_tag="VD788_13375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV77303.1"
                     /translation="MWLLSAVMLVNRAGAMVLPFIALYLTQERGMAVTAAGRVLSVYG
                     LGSVAGSWLGGWVSDRVGPDRALLASLAGSGVAFLCVGGLSDFWPITIGVFFLSVISE
                     AFRPACMSAMAHRAPAELRVRSFALLRLAANLGVGIGPAAGGVLALHDYRMLFVVDAL
                     TCWLAAWLLIRFPATRTTVEHAPVDGQAPARSPFADGPFLLLLALVTVLAIAFFQVFS
                     TLPLYLRESYGLREDAIGLLLAANAAIIVLFEMVLIHRAERYDRMSVVAVGAALVCLG
                     FGLMPFGQSMAWAAFTVVIWTIGEMLALPMLNVVAAERAGPLRHGRYLGLYTMAYSVA
                     FAIAPASGAWVYATVGTDSLWYAVGLLAVPLSAAFWLLRPVFRGPGRAAR"
BASE COUNT         2059 a         4610 c         4940 g         2087 t
ORIGIN      
        1 ccaaggatcg cccggtggtc acggtctgtc gcgctggcgt ggtgtcgcgg cgcgcggccg
       61 gtttgctcgc gaacctgggt ttcgacgctg ccagtctgca gggcgggatc cgtggctggg
      121 gcgcggtgtg gagcgaggcg ccggtccgga gtctcgatcc cgccaagggg tcgatgacgc
      181 aggtccgccg caacggcaag ggctgcctgt cgtacctgct cgcctcgcgc gacgaggcgg
      241 tcgtcgtcga tccgtcggtc gagcccgcgg cctatcgtca gctcgccgag cgcgagggcg
      301 cgcggatcac gcacgtgctc gagacccacg tccacgccga ccacctgtcg cgcgcccggg
      361 cgttgtgccg cgaggtcggc gcgcggttgc tgcttccacc caacgatcgg gtggatttct
      421 ccttcacgcc gttcgctcac ggcgagcggc tgcgcgtcgg cgagctggtg atcgaggcag
      481 tggcgacgcc cggtcacacc ggggaaagca cctgtttgct ggtcgatgat tcgatcctgc
      541 tgacgggcga tacgctgttc ctcgacggcg tcgggcggcc cgatctcgag gcgggggacg
      601 ccggcgcgga ggagggcgcg cgcgcattgc acgccagtct tcaccggcga ttgctcgatc
      661 gattcgacga cgtcctcatc tacccggcgc atcactcggg gccgatcctg ttcgatcacg
      721 caccgatcgc gacgaggctg tcggagctga ggtcgaagct cccgctgttg gcggcgcgcg
      781 aggacgactt cgtcgcggcg attctcggca ggctgcaggc gaagccgccg aacttcgacc
      841 gcgtcatcga gatcaacgag ggcaaggccg atctgggagc gatcgacgcg ctcgacatcg
      901 aggcggggcc gaatcgctgc gcggccggtt gagcgcgccg ccgacggaac gcgggatcag
      961 cccgccgccg ccttccgccg cgtccacttc gcgcgcaggc gcccgagcga gacgttgacc
     1021 gggtggttgc gtccgagcat cggcagcacc ggagccgccc gccggtccgt ccgcctgaac
     1081 agctgctgga gctcgcggtt ccccggatcc atctcgagcc ccagacggaa gctcgtgatc
     1141 gcccgcgtcc gcttgccggt gcgcaggtag accttcccga gattcaactg cagcaccggg
     1201 tcgaagtggt cgagcttcgc cgcctgttcg cacagacgca cgtcctcgac tttggcccgg
     1261 ccggcaagcg tccgactcaa accgagaaag gagcggtagc gcatttgggg gcggacggca
     1321 cccagctcgc gctcgagctg gacggccacc tgaaactgca gcgccgcctc cgcgtatcgt
     1381 ctgttctgga gcaggacgag cccacgctcg aagtgtcgga ctggtttgct tgttgccatg
     1441 gccctgaacc aaccggagat tgaccgatta tgtaggcgag ccggatcggt gtgtcaagcg
     1501 ctttcggccg gaggtccggc ggaggcccac gcatgccgga tcggccgtga cgtttttgac
     1561 cgggccgcgt cgaatcggca cagcggccgt gtcctctcca cccgccggct gtcttccggt
     1621 ttggacggag ttttcaggga ccgacggctt tggcggtgtt ttcgccggca aaaggctgac
     1681 gattttccgt ggtctcgaac ttgcattggc tgaagcagag cgccagagaa gcggatggac
     1741 ccgacggaga ataaatcgcc gtcgtcgcgg gttaacggcg gtgagggcga tccgacgggt
     1801 cggcagcggg ccgcgggcga cgagaggaac cagagcgagg ggctttggca ggtgaccaaa
     1861 ccgatgacca agtactacga cgactatcag gccagcggat cgttggcccc gcgcccgacg
     1921 cggcgtgagt ccgaccccga ctcgcgcggc gccgccccgc cgagcgccca actgctgccg
     1981 atctatttgc gcgagatggg ccagaccccg ctgctcgccc gggacgacga ggttcgcctc
     2041 gcgcgcgagc tgcaggaagc ccgtcaagcg atcgcgcggg tgatgtgcaa gctccccgcc
     2101 gactgccgcc gctacgtgct cgacggggat ctcggcgggc cgcgcgcggg ttatcgctgg
     2161 ccgctcgacc ggctggagcg ggcgtgcaag cggctgtcgg cctacgcgcg cgaccatgcc
     2221 gaatcgtcga tcgagccgct gctcgacgag gtccgccgcg acaagcgccg actggacgac
     2281 tcgcgcgacg cgctgatcgt ggccaacctg cgtctcgtca cccacatcgt caagaagtac
     2341 ggcaaccaag gcatcccgtt catggatctg atccaggaag gcaacatcgg tctgatgaag
     2401 gccgtcgaga agttcgagtt cgagcgcggc tacaagttct cgacctacgc ctattggtgg
     2461 atcaagcagg cgatctctcg cgcgatcgcc gacaaggctc gcatcatccg cattccggtc
     2521 cacatcaccg agaaggtcaa gcgcctcaag aaagtcagca acgagctcaa ggaaaccctc
     2581 ggtcgccagc cgacgagcaa agagatcgcc cgcaagatgc gcctgtccgt caagaaggtc
     2641 gacgagatcc tcggcgtggt ccaggatccg cagccgctcg acacgatggg tcccgaagaa
     2701 gaggccaacg gcgtgctgcc gttcgtcgcc gacgacaagg cggtcgatcc gctcgagcgc
     2761 acgctcgatc acgagatcac ctccaagatg cgctcggcgc tggacgtgct cgatccgcgc
     2821 gaggaagagg tgatccgcat gcgctacggc atcgggcgca agatgcggca cacgctcgag
     2881 gagatcggca tcgtgctcaa tctgtcgcgc gagcgcgtgc gccagatcga agcgatcgcg
     2941 ctgcgcaaga tcgaggccac cgaagagtcg cagtcgctgc gcggccacct gttctccaag
     3001 taacgatccc gcggtgaggc cacgctgggc ccgggtatcg ggtcggctcc gtcttgccgc
     3061 ccgccggtcg gcacctatat tcccctcatg tctcgcatcg ggcgatatct ggtcgcgtcc
     3121 gaagtcggac gcggcgcgat gggggtggtc tacctggcga cggacccgcg gctgaagcgc
     3181 caggtggcgg tgaagacgat gcggtggccc gacggtctca ccgagcggca gcaatcggag
     3241 ttccgcgagc gcttcctgcg cgaggcacac gcggccgcgg ctctttccca cgcgggcatc
     3301 gtcaccgtct acgacgccgg cgaggacgcg gcgagcgcgc aaccgttcat cgcgatggag
     3361 tacgtcgagg gctgtagcct gcgtcagcgg ctcgaccgcg acgggccgat cgaggtcggc
     3421 cgcgccgccg agatcgccgg cgccgtcgcc gacgcgctgc acgcggcgca cgagcggggg
     3481 atcgtccacc gcgacgtcaa gccggccaat ttgttgatcc gctcggccga cggcgcggtc
     3541 aaggtgaccg atttcggcgt ggcgcgcgtg accaactccg agctgaccca atccggcgcc
     3601 ggcctcggca cgccggccta catgtcgccc gaacagatcc gcggcgagcc gctcgacggc
     3661 cgcagcgacc tgttctcgct cggcgtcatc ctctacgagt cgctgagcgg cgagcggccg
     3721 ttcgatggcg aaacggtccc gtcggtgatc cactcgatca cgctcgaaac gccgattccg
     3781 atcaccaaac gaacgcccgg gctgccgcgc ggcctcgacc cgttcttcga ccgagcgttg
     3841 gcgaaacgac ccgccgaccg cttccccgac ggacatgcct tccggctggc gctcgaggcg
     3901 gcgctcggct gccgcgattc gccgcacacc gcgacggtcg gcgctctcgc ctcgggaacg
     3961 tcgcccgcgc ccgcgacgcc tctaccacct gccgaccgat cgctgccgtg gtggcgccgg
     4021 cggccgcggg tcgccgcggc tggcgcgctg atcgcgttcc tcgcggtctg gctgatcgcc
     4081 gcgtggggcg gcgacgccta cctgcatctc gaggccaaga gctcggtcga cgacggtgag
     4141 ctgaccgtgc tggtcgacgg gaccgaggtc tatcgccgct ggctcgatgc tcccgagcaa
     4201 cccaaggggc tgctcaagaa aatgctcgag cagagccacg agacgttcga ggcctacatc
     4261 gagattccgc ccggcaagcg cgaggtgacg gctcacgtcg tacacgacgg gcagagccgg
     4321 ccggatcgcg ccacgatcgt gctcgacatc gagcggggag aaacgcgcaa gctgagattg
     4381 gtggccggac gcgcgctggg ctcgtccgtg tccctcaagg tgcaatgaag cgaagagcga
     4441 caaacagcgg agggcagcga tgatgcagta cgacgatcca ccccggagcg agggaaccgt
     4501 cgtcgacccg cggctggcgc cacggcgact gcgcgagggt ctcgtcccgg aggacaccgg
     4561 tatctacctg cgggtcgagg aaggagcggc gagaggtacg gtcttcacgc tgtcggcggg
     4621 cggcgtctac gtcatcggtc gcgacggcgc ggacatcgcg attcccgacg agaaggtttc
     4681 gcgcaagcac gccgagatcg ggctgtacgg ccccggagcc tacgtgctgc gcgacctggc
     4741 gagcaccaac ggaaccctgc tcaacggcaa gcgcgtccag gagaaggcca ggttgagcaa
     4801 ctgggacgtg atccgaatcg gcgacacggt gatacgcttc cagatggtgg agggctcgat
     4861 cccgctcgag tcgtgagcgg ccggggccgg ccgtcccagg agcgcagcgt ggatcgcgac
     4921 cgcctgctcg aacgactcga acgatcggac gagccctggg acttcatcat cgtcggcggc
     4981 ggggcgaccg ggctcggggc gctggtcgac gccgcctcgc gcggctatcg cgcggtgctg
     5041 ctcgagcagc acgatttcgc caaggccacg tcgagccgca gcaccaagct gatccacggc
     5101 ggcgtgaggt acctcaaaga aggcaacgtc tcgctggtgc tggaagcgct gcgcgagcgc
     5161 ggcttccttt accgcaacgc gccgcacctg gtccgaaaca tggccttcgt catcccggtc
     5221 tactcctggt gggagggccc gttctacggt atcgggatga aggtttacga ccggctggcg
     5281 ggacggctcg gcttcaatcc gtcgcacacg ctgacacgcg aggagacgat cgagcggatc
     5341 cccacggtcg aaaccgacgg gctgctgcgc ggcgtggtct accacgatgg ccagttcgac
     5401 gacgcgcggc tcgcgatcga gttggcgcgc acggcggccg agctgggcgg gtgcccgctc
     5461 aattacatgc gcgtcgagcg cttgatcgac gggggtgggc tgttacgcgg tgtcgtcgcg
     5521 cgcgagctcg agggcgggtc ggtcttcgag atcgaggccc gcgcggtgat caacgcgacg
     5581 ggcgtgttcg ccgacgaact gcgccggctg gacacgcccg actgcgttcc gatgctggcg
     5641 ccgagccagg ggacgcatct cgtgctgccc aaggcgttcc tgcccggtga cagcgcgatc
     5701 ctcgtgccgc gcaccgacga cggccgcgtg ctgttcgccg tgccgtggca cgaccacgtc
     5761 gtcgtcggga cgaccgatac cgcggtcgac cgcgtgtcgc tcgagccacg cgcctcggcc
     5821 gaagagatcg agttcctgtt gacgaacgcg gcccgctatc tcagccgcga tcccggaccc
     5881 cgcgacgtgc tgagcgtctt ctccggtctc cgtccgctcg tcgccagggg cggagcccaa
     5941 gggacggcga acctgtcacg cgaccactcg atcgtgatct cgaattcggg gctggtgacg
     6001 atcaccggcg gcaagtggac gacctatcga aagatggccg aggaggtgat cgaccgagcc
     6061 gagatcgtcg ccgacctcga tcatcgacca tgccgcaccg agaccttgcc gattcacggt
     6121 cactgtacgc cgtccgccgc cgccgacgcg ctcggggtct acgggtacga ggcggccgcg
     6181 attcgggccc tggtcgaatc cgacccgacg ctcggtcggc cgctggcgcc cggcgtggcc
     6241 gtgatcggcg cgcaggtcgt gtgggccgtg cgtcgcgaaa tggcgcgaac ggtcgaggac
     6301 gtgctcgccc ggcggacccg cgccctgctg ctcggtgcgc gggcgagcgc cgaggcggcc
     6361 gatccgacgg cgacgctgat ggcgcgtgag ctcggtcggg acgaaagctg gtgccggcgg
     6421 acggtgcagg actatcggga gttggcccga ggctacgagc tgagcgtcta gtgtcgtcct
     6481 gtccgccgtg ctcggcggga actccccgcg gccgccggtg tccgttcgat ggagcgtcgc
     6541 ccgatcgtca ccaggagctg cacgtgagag taatcgccta cacttcgatt ccgttgatcc
     6601 tgctggccgc cgccgccctc gtcgggtcgt cgccacacgg cgccggcgcc ggtgaggagg
     6661 ctcgcgcgac gaccacggtc gagccgggcc tcacgcgcgc cgaggcgctg ttcgcggagg
     6721 gctcctacga gtcggcgctc gagatctacc gcgcgatcga tctcgacgcg ctcggcgacg
     6781 aggagcggcg atgggtgcgg atgcggctgg cagacgccgc gtggcgctcg gcggcggcga
     6841 cggacggcgc cgacgaacag cggctcgagc aggcgcggca ggcgctcgag gcagagattc
     6901 gcgaccgcac gcgcgaggac gagcaagacc gcgtctgggc cgaggtccag gaatcgctcg
     6961 gtgattactg gtggacccga cgcggctggc cgaactgggg ccaggcctgg cagcactacg
     7021 acgcggcgct cggctggtgg gccggttcga gcgagatcga gctggcgcgc gagcgctacc
     7081 tgtcgatcgt ctggcgcgcg gcgcgcccac ctcacgtccc gccccactac tactacgggt
     7141 attacggcaa tcagctcccg caaagcgtgc tggagaacgc ccgcaagatc gccgtcgacc
     7201 cgcgcgaccg ggcgcacgcc gcctacctga tcgccatggg tctgcggttc cacggcggcg
     7261 acccggcgtc ggtgcggcgt acggcccgag cgttcgacga agcggtcgag gccggccgtg
     7321 gcacggactg gtacgacgac gcgctgttcc accacgcgga gtggctggcg ggcagcggca
     7381 gggtcgtcca cgacgagcgg ggcgaggtca ccgcgctccc cgacttcgtt cgcgccgtcg
     7441 agcagtaccg ccgactgctg cgcgagttcc gtaagggaga gacgcgctac tacgaccagg
     7501 cggaggcccg tgtcgagcag atcacggcgc cggaggtcgg cgtggtcgtc tcgaacgtct
     7561 tcctgccgga cgccgaggtt cgctacgagc tcggttaccg caacgtggaa cgggtcgagc
     7621 tcgcgctcta tcccgtcgag ctgacccgcg atctccgctt cgacacgaat gacgggtccg
     7681 acacttggct cgacgggctc gccgcggtca agggcaggcc gtggcaccgc tggagccacg
     7741 atacgggaga cgccggcgag cacgtgccgg gccgggcggc cctcatgctc gaacgcgagc
     7801 ccgcgccggg cgcctacctg ctcgaagcga gcgccggcgg cgagacggcc cgcgatctgg
     7861 tgctggtcag ccgggtggcg atcgtgcaga aggtgtccgc cggccgagcg ttggtctacg
     7921 tctgcgacgc gatcgacgga tcgccccggg gcgcggccga ggtcacgcta tgggtccggc
     7981 gagagcagag ctgggagcgc ctcgcgggcc ggaccgacga gcagggcctg tcggcgttcg
     8041 agcttcccgc cgccggcgac cgcagcgaga ccttcgccgc ggcgcacgcc ggggacgatc
     8101 aggccttcag cgccggtcac gcctggcatc accggtcgga gtccgtgccg tggcgcatct
     8161 acgccttcac cgaccggccg gcctaccgcc ccgaggatcg cgtgcggttc aaggtcgtcg
     8221 cgcgacaggt agtcgacggg ggctaccgta cgcccgacgg tcggaccttg cattacgaga
     8281 tcgtcgaccc gcgtggcacc gagttggcgc gcggcacgct cgagctgaac gccttcggta
     8341 cggcgtgggc cgagctcgcc atggtcagga cagccacgct cggcgagtac cgcatcgatt
     8401 tccgcgagca ggaagagtcc ggctcgatcg gatcggcggc gctgttccgg ctcgaggagt
     8461 acaagctgcc cgagttcgag gtgtcggtcg agacccccac ggccgacggg cggcggctcg
     8521 cgtttcgcgt cggcgacacg gtcgaggccg acgtggccgc ccggtactac ttcggcggtc
     8581 cagtggcggg cgccgaggtc gaggcggtga tctaccagaa gccgctgtgg gtctggtggg
     8641 aaccgccccg cgagttccaa tggttgtacg aggacgacgc gcgatcccgt cgccagcact
     8701 ggggcggacc gggccagatc gtcgagcgtg tccggctgac gaccgacgcg agcggtcggg
     8761 ccacgctccg cttcgacaca cctgcggccg gcggccagga cttcgaatac acgatcgaag
     8821 cgcgcgtccg cgacgcctcg cgtcgcgagg tcgaggggcg tgggacggtg cgggtcagcc
     8881 gccagcgcta cttcgccttc gtccgggcgg agcacaacct ctatcggccg ggcgacgagg
     8941 tgcgtgtcga gctgaagacg atcgacccga acggcgagcc gatcggcgcg gacgggcgcg
     9001 tgcgagtcac acgcgaggta tggcgcgaga tctggtccga cccggccggg cgtgaggtca
     9061 gcgggcacga gctgccgccc gccgaacgcc gggggcgggg ctggacgttg acttcgtcgg
     9121 gctaccggcc cgtcgagatc gtcgacacgg ccgtgcgcac gggcgaggac ggatcggccg
     9181 agttccgctt cgaggcggac gaggccggct attaccgggt cgccttccgc ggagacgacc
     9241 tggccgacta tcccgtgcgc ggcgagacca cggtgtgggt cgccgattca cggacacgca
     9301 ccgtcggcta ccaccacggc ggtgtcgaga tcctggtcga ccgcgacacg ctgcgcgccg
     9361 gtcagtcggc gccggtgatg atcagcgcgc cggtcgaggg tgcgtgggtc ctgttcagca
     9421 tcgagggcga ggagttgtac gactaccgca tcgtgcatct cgacggttcg gtcaagttga
     9481 tcgaggtcga gatcgaaccg cgctacgtgc cgaacgtgtt cttcgaggtc gccttggtgc
     9541 acgacggcga gctgtggctc gacagccgtc gggtggtcat cccgcccgtc gagcagttcc
     9601 tcgacgtcgc ggtcgaagcg gaccgcacgg agtacgggcc gggcgacacc ggacgtttac
     9661 tgctgacgac acgcgaccat gagggccagc cggtctcggc cgaggtcgcg ctggcgctgg
     9721 tcgacgcgtc ggtgttctac atcgagcagg acctggccgg ggacccccgc cggttcttct
     9781 tcggcgacag ccgggcgaag ctcgtggtca ccggcagcac gttccagcag aagccgtacg
     9841 tacggcgcga cggcgggcac gacgatcggt cggtcgacct gggtgacgag ctgtcgcggc
     9901 ggtacgacgt cgagggactc tccgcgcggg aggctgccga gaccgacgcg ctcgaggtcg
     9961 tggcaacgcc ggccccctca ccggccgtcg gagcgaagat gcggcaggag gcggagggcg
    10021 ccgagatctc gacggtcgag gtgcggaccg atttccgcgc gaccgccgtc tggcagccgg
    10081 aggtcgtcac cgacggcaac ggtgcggcca cggtcgaggt ggtcttcccc gacacgctga
    10141 ccgcgtggcg ggcgaccgcg cgcgtgacgg ggcgcgacga tcggttcggc atcgccgagg
    10201 ccgagatgcg taccgacaag ccgctgatcg tgcgtctgca ggcgccgcga ttcttcgtcg
    10261 ccggggacga gacgactgtc tcggcggtgg tcaacaaccg gaccgacgac gatctcgagg
    10321 tcacgacgag cctcgacgtc acgggcgccg tgcgcgacgg cgcggccgac cgcgtgttgc
    10381 gggtcacggc gggcgccgaa gcgcgggtcg attggcggct gcgcgccgaa caagcgggcc
    10441 agctcgagct gaccgcgcgc gccaccgcgg gggagctggc cgacggcatg cgcaaccgct
    10501 acccgatcca cgagcacggg atcgacaagc gggtctaccg ggccggcaag ctgcgcggcg
    10561 aggcggtcga gatcaagctc gacctgccgg cggaacgtcg atcgacgacg atgagcgtgc
    10621 aggtcacgcc gagcctggcg atcacgatgc tcgacgcgct cccgtatctg atcgactacc
    10681 cttacggctg caccgagcag acgatgagcc gcttcctgcc gaccgtgatc gtggccgata
    10741 cgcttgccga gctgggtctc gaccggacgc agatcgcgcg acgcctgttc ggcggtatcg
    10801 agccgcagca cgccgcacgg acccacaccg ggaacgagcg ggcgctcgag cggctggacg
    10861 agatggtgcg cgccggtctc gaccggctct acgatttcca gcacgacgac ggcggatggg
    10921 gctggtggaa ggtcggcgac agcgacccct acatgagcgc ctacgtcgtt tggggtctgt
    10981 cgctggccaa cgatgccggg gttcgcgtcc gctccgacgt gctcgagcgc ggggtcgcgt
    11041 ttctcgaccg cgagctggtc gaggccgagg agcggcccga cctgcaagcg tggatgctgc
    11101 acgcgctcgc cgtcgcgacg gagcgtgggg gcccgggccc gtttcagcgg gcggcgctcg
    11161 acaatctgtg ggtgcggcgc gacggcttga acgcctacac gcgcgcgctg ttcgcgcttg
    11221 ccgcgcacga gctgggtcag accgagcggg ccgaaacact ggtgcgcaac ctggcgaacg
    11281 gcgtcgagcg cgatcgcagt cccgacacct cgatcgtgag ccgcggggcc ccgcacggaa
    11341 gcgacgccgt gatcggcacc gcgcactggg gcagcgaccg attctactgg cgctggtccg
    11401 agggtgcgat cgaggccacc gcgttcgcgc tgcgcgcgtt gctcgcgatc gatcccgaga
    11461 acgagctggt cgagccggcg accgagtggc tgatccgcaa tcgacgcggc gcgcagtgga
    11521 gcaacacgcg cgacaccgcc ttcgtcgtgt tggcgttgac cgactacctg accgcgagcg
    11581 gcgagctgtc gcgcgaggcc gagtacgagc tgttcgtcaa cgatcgctcg atcgcgacgc
    11641 ggcgggtcgg cccgcgcgac ctgttcgacg cccccagccg cttcgtcgtc gatccgacgg
    11701 cgatcgagga cggcgagaac cgcatccgca tcgtgcggcg ctcgggcgat gcggcgctct
    11761 acttcgccgc ggaggccacg ttcttcagcc tggaagagcc cgtcactccc gccggcaacc
    11821 aggtcttcct gcgccggcag taccaccgcc tcgccgcgcg accgacgttg ctcgagggct
    11881 acgcgacgcg gctcgtgccg ctcgacgatt tcggcgaggt cgcgagcggc gagcgcgtcg
    11941 aggtggtcct cacgatcgaa gtcaagaacg actacgagta cctggtgatc gaggatctca
    12001 agccggccgg gctcgaagcg gtccagctcc gaagcggcga gccgatccac gcgcgcgagc
    12061 tggcgccgag cgcggcggcg cggccggagc gctcacgcga gccgaccgac tacaccggcc
    12121 gctcgcggcc gatgcatcaa gagctgcgcg atcgtcgggt cgcgttcttc atcgacgact
    12181 tgccgcaggg cgtgtgggag gtgcgctaca cgctgcgcgc cgagctgccc ggccggttcc
    12241 acgcgctgcc ggccacgatc catgcgatgt acgtgccgga gatccgtgcc aacggcgccg
    12301 agcttcgcct gggggtgacc gactgagggg catcgtccga tcctatcggg cggcattcgc
    12361 cggcctgccg cgggaggtgt ggctgctgtc ggccgtcatg ctggtcaacc gcgcgggggc
    12421 gatggtgctc ccgttcatcg cgctctatct gacacaggag cgcgggatgg ccgtcacggc
    12481 ggcggggcgc gtgctgagtg tctacgggct ggggtcggtc gccggctcgt ggctcggcgg
    12541 ctgggtcagc gaccgggtcg gacccgaccg cgccctgctc gcgagtctgg ccggcagcgg
    12601 ggtcgccttc ctgtgcgtcg gcgggctgag cgatttctgg ccgatcacga tcggtgtttt
    12661 cttcctgagc gtgatctccg aggcgtttcg tccggcctgc atgtcggcga tggcgcaccg
    12721 cgcgcccgcc gagctgcgag tgcgcagctt cgccttgctc cggctggcgg ccaacctcgg
    12781 cgtcggcatc gggcccgcgg ccgggggtgt gctggcgctg catgactacc ggatgctgtt
    12841 cgtcgtcgac gcgttgacct gctggctcgc ggcgtggctc ttgatccgct tccccgcgac
    12901 ccgcacgacc gtcgagcacg cgccggtgga cggacaggcg ccggcgcgct cgcccttcgc
    12961 cgacgggccc ttcctgctgc tgctggcgct cgtcaccgtt ctcgcgatcg cgttcttcca
    13021 ggtgttcagc acgctgccgc tctaccttcg ggaatcctac gggttgcgcg aagacgcgat
    13081 cggcctgctg ctggcggcga acgccgcgat catcgtgctg ttcgagatgg tgctgatcca
    13141 ccgcgccgaa cgttacgacc gtatgagcgt cgtcgcggtc ggtgcggcgt tggtctgcct
    13201 cggcttcggc ctgatgccgt tcgggcagtc gatggcgtgg gccgccttca ccgtcgtgat
    13261 ctggaccatc ggcgagatgc tggccctgcc gatgctcaac gtcgtcgccg ccgagcgggc
    13321 aggccccctc cggcacggtc gctatctggg gttgtacacg atggcctact cggtcgcgtt
    13381 cgcgatcgca ccggcgagtg gagcctgggt ctacgcgacg gtcggcaccg acagtctgtg
    13441 gtacgccgtc gggctgctcg ccgtcccgct gtccgccgcg ttctggctgc tgcggcccgt
    13501 gttccggggt cccggtcggg cggcgagatg aatcgcgccg ctttgggtat aatgaaccgt
    13561 acccggcggg gcgctcccgc gggtcgttga tcacgcgcca agatcggcgc agcacgaaga
    13621 caaggcgaag aggctccgat cgtcttcatc attccgggcc gggccgcggg tcgggttcgg
    13681 ggcggtatcg accgcg
//
LOCUS       DASPGN010000458         5822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_904911, whole genome shotgun sequence.
ACCESSION   DASPGN010000458 DASPGN010000000
VERSION     DASPGN010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904911"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..561
                     /locus_tag="VD788_13380"
     CDS             214..561
                     /locus_tag="VD788_13380"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HXV77304.1"
                     /translation="MANQEPGSGKKKMTARKTTKRAAKAVATKKRDVSAAKPKPTGRQ
                     RARAGATSSDAPVPAERPRILTWQRRHEMIAEAAYYRAERRGFVGGDPADDWREAEAE
                     VDRLLIEHPERLD"
     gene            591..1562
                     /locus_tag="VD788_13385"
     CDS             591..1562
                     /locus_tag="VD788_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77305.1"
                     /translation="MQSRDGNDPRHEPRQTSLVADPLRPQLERLGLGAADGGPPPEVP
                     EEVRFGDQTLRIDGWERMVMRAHGLGTDGRGRPWPALVSRAVAFRARAFELAARRPPA
                     DTSTGRAGSIERLRDDHALAGALLGELEWTQRRLRDEGEDRLATRFTELIDRVDRIAR
                     QLGVLLGDGAVAALPRDVGVSLDPTYRDRWPIPRREAGPVDSHRPSPDRSERLRLKLR
                     VGRLPAIAIVLVAVAVSVVAWLWPVGPRSSGQSAAGLGPMFQRPAVVDAVAEAGLVTV
                     TVDGVVWDGLSVERRESETATLTEIARTAGYGSIRLRTADGAVIAVP"
     gene            complement(1593..3002)
                     /locus_tag="VD788_13390"
     CDS             complement(1593..3002)
                     /locus_tag="VD788_13390"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HXV77306.1"
                     /translation="MHWGAVTIEIGVLAVAFVVLAWELSFGQRGPNARRTGYAICATG
                     LLGLLVLSSRLPVDVTFTEALVQDPFALYVKQLLLLAALLTVLASAPYADRRGWSSRS
                     GEFLTLLLFATVGGMALVSAREFLTLFVAFELLSLPLYSLAAIEKNRAESPEGAVKFF
                     LFGSVSSAMLLLGLGLLFAAGGTTFWLDVLRLDSPAARVGLVLLLVGFGFKIAMFPFY
                     MWVPDTYQSAPTPFVAFLSVAPKAAGVAALFRLQFEVFAVHVPDVTRWVAVLAGGTMV
                     VGNLLALPQTQLKRLLAYSGVAQIGYVLLALAAGTRLGAGMALFFFVAYLFSNMGAFF
                     AVAAIESSGEQPTCYGVRNLIRRSPLLAAAFIVFLLSLGGIPFALGFWGKMYVFLAAG
                     EAGLWKLVLLGALLAVVALYYYLNVVRFMLIVPDKGPSVRVPLPLALALLACSVLVVA
                     GGLAPQWFVEPALRAVSGF"
     gene            complement(3002..4543)
                     /locus_tag="VD788_13395"
     CDS             complement(3002..4543)
                     /locus_tag="VD788_13395"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXV77307.1"
                     /translation="MSDHLLSIVLLSPFAGGFAILLVPSSARGLLRGVAIASAIPPLV
                     LSIVLLAGYDTASGGFQFVERYPLVPSLGIQYLLAVDGLSLSLVLLTAFIYLTGITTT
                     WYLGEREKEFFLFLALLVTGVFGVFLSLDLFLFFLFYELAVLPMYVLIGVFGSSMEVP
                     GRGPFGWTFGALRVGIKEYGAMKLTLYLLAGSAFILVGLFLLWVESAELLPQATFDYL
                     ALETIGRDLPSELATVMFLLFYLGFGILAGIWPFHTWSPDGHAAAPAAGSMMHAGVLM
                     KLGAYGVLRVGFALLPDAAAELAWFVGTVAVINIVYGAIAAGAQTDIKYLIAYSSVSH
                     MGIVMLGLATLNEPGVAGAVFQMVAHGIMTGLLFTLVNMIYAKAHVRDMTKMGGFAGR
                     QPGLATLFMLAGLSSLGLPGLAGFVAEFLVFLGAYTSAHRWWVFPAVIGAFITAIYVM
                     RAMNRIFLGPPNPDFDKLEDARGMEWVTLVVLGGMLVVFGLYPRLLLDSIQGGVVEFL
                     ARWGA"
     gene            complement(4547..>5822)
                     /locus_tag="VD788_13400"
     CDS             complement(4547..>5822)
                     /locus_tag="VD788_13400"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HXV77308.1"
                     /translation="TLNIRHSIELLIHEGDANPIPLFVVALLVFAGAMGKSAQFPLHV
                     WLPDAMEGPTPVSALIHAATMVAAGVYLMARVTPLLDQTPQLAAWVLAIGALTAFLAA
                     TMALVERDVKRILAFSTISQLGYMMAAVGAAGTVAAVYHLSTHAFFKALLFLAAGSVI
                     HALRTNDIFQMGRLARRMPLTTTYFLIGGLALSGVPPLSGFFSKDAILVDLLHDGRRF
                     AFWLLVVTAGLTSFYMGRAFFVIFFGGRDGAGHAHESPAVMTVPMGVLALLALGGGLF
                     GSTIAELIEHSISSGAGRAERSHSLFVPLVKTAVAVAGIVLAWLGYQARAFEPAALAR
                     RFAPITRLLERRYYVDDLLYALYRALYLGAGAVVGWFDRYIVDGLVNLVSWIGWRLAA
                     RARGVQTGRAQDALYAIAVGFLLLALLALGRW"
BASE COUNT         1132 a         2029 c         1996 g          665 t
ORIGIN      
        1 cgagcgcgca ctggtccgcc gagtggccga cggatgggag tcggtgctgc tcgagccgtg
       61 aaagccggga tttcccgcgc cgccgccagc ggtcatcggt cgcactagag acctcggccg
      121 ggagttgcta cgttggtacc caccgcaacc gatggagcgg ggttcggccg ccccgccgga
      181 cgaccgatgg acaaccgaag cggagtatcg acgatggcga atcaggagcc cggatcgggc
      241 aagaagaaga tgaccgcacg caagacgacc aaacgcgcgg cgaaagcggt cgccacgaaa
      301 aagcgggacg tgtcggccgc caaaccgaag ccgacgggga ggcaaagggc ccgcgccggc
      361 gcgacctcgt ccgacgcgcc cgttccggcc gagcggccgc gtatcctcac ctggcagcgc
      421 cggcatgaga tgatcgccga ggctgcgtac tacagagccg agcggcgcgg cttcgtcggc
      481 ggcgacccgg ccgacgattg gcgcgaagcg gaagccgaag tcgaccggct gttgatcgag
      541 catcccgagc gcctggattg agggccgacg ggcccgccga ggacgaccgg ttgcaatcga
      601 gggatggcaa cgatccccgc cacgagccgc ggcaaacgtc gctcgtcgcc gatccgctgc
      661 gaccgcagct cgagcgcctc ggtctcggcg ctgcggacgg cgggccgcca cccgaggtac
      721 ccgaggaggt ccgtttcggc gaccagacgc tgcgcatcga cggctgggag cggatggtga
      781 tgcgcgccca tggtttgggt accgacgggc gcggccgacc gtggcccgcg ctcgtctccc
      841 gtgcggtcgc tttccgcgcg agggcgttcg agctggcggc gcgccggccg ccggccgaca
      901 cgtcgaccgg ccgggccgga tcgatcgagc ggctacgcga cgaccacgcg ctcgccggag
      961 cgttgttggg cgagctcgag tggacccagc ggcgcctgcg ggacgagggc gaggaccgtc
     1021 tggcgacgcg attcaccgag ctgatcgacc gcgtcgatcg aatcgcccgg cagctcggcg
     1081 tcctgctcgg tgacggcgcc gtcgccgctc tgccccgcga cgtcggggtc tcgctcgacc
     1141 cgacctatcg cgaccgctgg ccgatcccgc ggcgagaagc cggccccgtc gattcgcacc
     1201 ggccgtcgcc cgatcgatcc gagcgcctgc ggttgaaact gcgggtcggc cgactgccgg
     1261 cgatcgcgat cgtgctggtc gcggtcgcgg tgagcgtcgt cgcctggctg tggcccgtcg
     1321 gaccgagatc gagcgggcag agcgccgccg gtctgggccc gatgttccag cgccccgcgg
     1381 tcgtcgacgc cgtcgccgag gcgggtctcg tcaccgtcac ggtcgacggt gtggtgtggg
     1441 acggtctgtc ggtcgagcgg cgcgagtcgg agaccgccac cctgaccgag atcgcacgca
     1501 ccgccggcta cggctcgatc cggctgcgga cggccgacgg ggccgtcatc gccgtcccct
     1561 gagccgaggg ccggaggtta ccgcgacaga cactagaagc ccgagacggc gcgcagcgcc
     1621 ggctcgacga accactgcgg cgccaatccg ccggccacca ccagcaccga gcaagccaac
     1681 agcgcgagcg ccagcggcag cggtacgcgg accgacggcc ccttgtcggg cacgatcagc
     1741 atgaagcgga cgacgttgag gtagtagtac aaggcgacga cggcgagcaa cgcgccgagc
     1801 agcaccagct tccacagccc ggcctcgccc gcggcgagga agacgtacat cttgccccag
     1861 aatcccagcg cgaacgggat tccgccgagc gacaacaaaa agacgatgaa tgccgccgcc
     1921 agcaacggcg agcggcgaat caggttgcgc acgccgtagc aggtcggctg ctcgcccgag
     1981 ctctcgatcg cggcgaccgc gaaaaacgcg cccatgttcg agaacaggta ggccacgaag
     2041 aagaacagcg ccatgcccgc gccgagacgc gtcccggcgg ccagcgcgag cagcacgtag
     2101 ccgatctggg ccacgccgga ataggccagc aggcgtttga gctgggtctg cggcagcgcc
     2161 agcaggttgc cgaccaccat cgtcccgccg gccagcaccg cgacccagcg cgtcacgtcc
     2221 ggaacgtgca ccgcgaacac ctcgaactgg agccggaaca gcgcggcgac tcccgccgcc
     2281 ttcggcgcga ccgacaggaa cgcgacgaag ggcgtcggtg cggactggta ggtgtcgggt
     2341 acccacatgt agaacgggaa catcgcgatc ttgaaaccga agccgacgag cagcaatacg
     2401 agcccgaccc tggccgccgg cgaatcgagg cggaggacgt cgagccagaa ggtcgtcccg
     2461 ccggcggcga acagcagacc gagcccgagc agcaacatcg ccgacgaaac gctgccgaac
     2521 aggaagaact tgaccgcgcc ctccggcgat tccgcccggt tcttctcgat cgcggccagc
     2581 gagtagagcg gcagcgacag cagctcgaag gcgacgaaca gcgtgaggaa ctcgcgcgcc
     2641 gagacgagcg ccatgccgcc caccgtcgcg aacagcaaca gcgtgaggaa ctcgccggaa
     2701 cgagacgacc agccgcgccg gtcggcgtac ggtgcgctcg ccagcaccgt cagcagcgcc
     2761 gccaacagca gcagctgctt gacgtacagc gcgaacgggt cctgcaccag cgcctcggtg
     2821 aacgtcacgt cgaccggcag ccgggaggat aagacgagca ggccgagcag gccggtcgcg
     2881 cagatcgcgt aaccggtccg acgcgcgttc ggcccgcgct ggccgaagga cagctcccat
     2941 gccagcacga cgaatgcgac ggccagcacg ccgatctcga tcgtcaccgc gccccagtgc
     3001 attaggcgcc ccagcgcgcc aggaactcga cgacgccgcc ctggatcgaa tcgagcagca
     3061 accgcgggta gagtccgaag acgacgagca tcccgccgag cacgaccagc gtgacccact
     3121 ccattccgcg tgcgtcctcg agcttgtcga agtcggggtt cggcgggccg agaaaaatcc
     3181 ggttcatcgc gcgcatcacg tagatcgccg tgatgaacgc gccgatcacc gccgggaaga
     3241 cccaccagcg gtgggccgag gtgtaggcgc cgagaaagac gaggaactcg gcgacgaacc
     3301 ccgcgagccc gggaagtccg agcgacgaca gaccggccag catgaacaac gtggccaggc
     3361 ccggttgccg cccggcgaaa ccgcccatct tcgtcatgtc acgcacgtgg gccttggcat
     3421 agatcatgtt gaccagcgtg aacagcaacc cggtcatgat cccgtgcgcc accatctgga
     3481 acaccgcgcc ggcgaccccc ggctcgttca acgtcgccag ccccagcatg acgatgccca
     3541 tgtgcgacac cgacgagtag gcgatcaggt acttgatgtc ggtctgggcg ccggccgcga
     3601 tcgcaccgta gacgatgttg atcacggcga ccgtgccgac gaaccaggcc agctcggccg
     3661 cggcgtcagg tagcagcgcg aacccgacgc gcagcacgcc gtaggctccg agcttcatca
     3721 acaccccggc gtgcatcatc gacccggcgg ccggtgccgc ggcatggccg tcgggcgacc
     3781 aggtgtggaa cggccagatg ccggccagga tgccgaagcc gaggtagaac aacaggaaca
     3841 tcaccgtcgc cagctcggac ggcaggtcgc ggccgatcgt ctcgagcgcc agatagtcga
     3901 aggtcgcctg cggcagcagc tcggcgctct cgacccacag caggaacagc ccgacgagaa
     3961 tgaacgccga cccggccagc aggtagagcg tcagcttcat cgcgccgtat tccttgatgc
     4021 cgaccctgag cgcgccgaac gtccagccaa acgggccccg ccccggcacc tccatgctcg
     4081 agccgaagac gccgatcaac acgtacatcg gcagcaccgc cagctcgtag aacaggaaga
     4141 acaggaacag atcgagcgac aggaagacgc cgaagacgcc ggtgacgagc agggccagga
     4201 acaggaagaa ctccttctcg cgctcgccga gataccaggt cgtcgtgatt ccggtgaggt
     4261 agatgaacgc ggtcagtagc acgagcgaca ggctgagacc gtcgaccgcc agcagatact
     4321 ggatcccgag cgacggcacc agcggatagc gctcgacgaa ctggaagccg ccggacgcgg
     4381 tgtcgtagcc ggcgagcagc acgatcgaca gcaccagcgg cgggatcgca gatgcgatcg
     4441 ccacgccccg cagcagcccg cgcgccgacg acgggacgag cagaatggcg aagccgccgg
     4501 cgaacggcga cagcagcacg atcgacagca ggtgatcgct catccgtcac caccggccga
     4561 gcgcgagcaa cgcgagcaac aggaagccga ccgcgatcgc gtagagcgcg tcttgcgcgc
     4621 ggccggtctg cacgccgcgc gcgcgcgccg ccagccgcca accgatccag ctgacgaggt
     4681 tgaccaaccc gtcgacgatg taacggtcga accaaccaac gaccgcaccc gcgccgaggt
     4741 agagcgcgcg gtacagcgcg tagagcaggt cgtcgacgta gtagcgccgt tcgagcagcc
     4801 gggtgatcgg cgcgaaccgc cgggcgagcg ccgccggctc gaacgcgcgg gcctggtagc
     4861 cgagccaggc gaggacgatt ccggcgacgg ccaccgccgt cttgaccagc ggaacgaaca
     4921 gcgagtgcga tcgctccgcc cggcccgctc cgctcgagat cgaatgctcg atcagctcgg
     4981 cgatcgtcga accgaacagc ccgccgccca acgccagcag cgccagcacg cccatcggca
     5041 ccgtcatcac ggccggtgat tcgtgcgcgt gcccggcccc gtcacgcccg ccgaagaaga
     5101 tcacgaagaa cgcacgcccc atgtagaacg aggtcagccc ggcggtgacg acgagcagcc
     5161 agaaggcgaa ccgccgtcca tcgtgcagca gatcgaccag gatcgcgtcc ttcgagaaga
     5221 acccggacaa cggcgggacg ccggacagcg ccaggccgcc gatcaggaag taggtcgtcg
     5281 tcagcggcat cctccgcgcc agccgaccca tctggaaaat gtcgttggtc cggagcgcat
     5341 ggatcacgct gccggccgcc aggaacagca gcgccttgaa gaacgcgtgt gtcgacagat
     5401 ggtagacggc ggcgaccgtg cccgccgcgc cgacggccgc catcatgtag cccagctggc
     5461 tgatcgtgga gaacgccagg atgcgcttca cgtcgcgctc gacgagcgcc atcgtcgccg
     5521 ccagaaacgc ggtcagcgcg ccgatcgcca gcacccaggc ggcaagctgc ggcgtctgat
     5581 cgagcaacgg ggtgacgcgc gccatcaggt agaccccggc ggcgaccatc gtcgcggcgt
     5641 ggatcagcgc ggagaccggc gtcggcccct ccatcgcgtc gggcagccag acgtgcagcg
     5701 ggaactgggc gctcttgccc atggccccgg cgaacacgag cagggccacc acgaacagcg
     5761 ggatgggatt ggcgtcgccc tcgtggatga ggagctcgat cgagtgacgg atgttgaggg
     5821 tc
//
LOCUS       DASPGN010000459         6606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1607766, whole genome shotgun sequence.
ACCESSION   DASPGN010000459 DASPGN010000000
VERSION     DASPGN010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1607766"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VD788_13405"
     CDS             <1..954
                     /locus_tag="VD788_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXV77309.1"
                     /translation="GSDEFEPKLSLVPLAVGTLKGTLYSLVLAIPLGVLGAMYTSQFL
                     HHRLRRIVKPTVEIMAALPSVVLGFLAGLWLAPRVERLMPALALMLVVIPVLVVAGGV
                     LWRRLPGAARSLLPDGSEAIVQVGIVTAAVALCLWLNHPFESVVFAGDFQQWLLDVTG
                     VGYDQRNAVIVGLAMGFAVIPIIFSIAEDSFSNVPRTLVSGSLALGANRWQTVTRVVL
                     PSASPGIFSAIMIGFGRAVGETMIVLMATGNTPLMDWNPFNGFRTLSANIAVEIPEAP
                     QSGTLYRTLFLAALLLFVFTFLTNTVAELVRQRLRQRYAQL"
     gene            965..2611
                     /gene="pstA"
                     /locus_tag="VD788_13410"
     CDS             965..2611
                     /gene="pstA"
                     /locus_tag="VD788_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXV77310.1"
                     /translation="MKKRRSGGRVDWLGDGLTSLCAGALALNVLLVIALLCILVVNGM
                     GYFWQRPLVEMTVEDGMVYLGEIQERESIPATDDDGTAAGSRVRIKVGNRDLTGVDFV
                     WIDEPRVESRRTPDDAVLLERLEYGNFYGFLERLQRGDEVLAEGSAEVWEAFQSLHAD
                     KLREATLIRRLEKREIGDVNHAMERLRLAERRIDRSELDPSEREQRLARLRQEQALAE
                     SEYEALSRRLFDMRERWAAETLMVRAVNGALQQIPVANVVRAVRPNQLGTTGRLALYL
                     ERIWEFLSSDPRESNTEGGIFPAIFGTVMMVFLMSFAVAPLGVLAALYLREYAKQGPL
                     VRTVRIAVNNLAGVPSIVFGVFGLGFFVYLVGGSIDRLFYPESLPTPTFGTGGILWAS
                     LTLALLTVPVVIVATEEGLAAVPRIVREGSLALGATKFETTWRVVVPAAAPGILTGLI
                     LAMARAAGEVAPLMIVGMVKLAPALPVDHHFPFVHFERKFMHLGFHIYDVGFQSPNVE
                     ATKPLVFATALLLILVVTAMNLLAIGARNHLRRKYMGSAV"
     gene            2628..3542
                     /gene="pstB"
                     /locus_tag="VD788_13415"
     CDS             2628..3542
                     /gene="pstB"
                     /locus_tag="VD788_13415"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HXV77311.1"
                     /translation="MTGELRAEKFGSDDTRAPADRPAGGRGPDAGRPSRTDPAAPGGD
                     ERTAITPDIADPILDIRALSLWYGSNQALHDISLLIGQRKITAFIGPSGCGKSTLLRC
                     LNRLNDMIDEVRVEGQILFESEDIRNPAADVNALRTRIGMVFQKSNPFPKSIYDNVAY
                     GPRIHGIRDRRQLDEIVERSLRGAALWDEVKDRLKHTALGLSGGQQQRLCIARAIAIE
                     PEVILLDEPCSALDPIATAKIEELMIELKERYTLVIVTHNMQQASRVSDFTAFMYLGR
                     LVEYQPTDRLFINPIRKQTEEYITGRFG"
     gene            3561..4235
                     /gene="phoU"
                     /locus_tag="VD788_13420"
     CDS             3561..4235
                     /gene="phoU"
                     /locus_tag="VD788_13420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXV77312.1"
                     /translation="MDQSAVQLREQLDLLSELILRLGGMAEDAIGKSVRALVDRDSDV
                     GREVIEGDKEVDALELEIDGLCMSILARHQPMAGDLRFVATAMKITPDIERIADHSVN
                     ISERALELNDEPPLDALIDLPMMAARVQQMVRGALDAFVRRDAKVALDVIRMDAEVNR
                     RMEQVFRVLLSYMLSDPTTISRALRTTFVAKYFERIGDQATNICEQIVYMTDARVIKH
                     QAFPND"
     gene            4244..4936
                     /locus_tag="VD788_13425"
     CDS             4244..4936
                     /locus_tag="VD788_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV77313.1"
                     /translation="MKRIVIIEDDADIALSIKYSMEREGEFAVTVARDGEAGIEAATR
                     SVPDLVLLDLNLPGLDGTEVCRVLRSNRSTSAVPIIMLTARVEEREKVTGLDLGADDY
                     ITKPFSIREVLARVRAVLRRSAGGSRGHKPLAFGPLTLDEARRRVTVDGEEIRLTRKE
                     FDLLADLMRRPDLVLTRELLLARVWGYDHPGATRTVDVHIRQLRKKLGQAAARYIETV
                     VGVGYRFRGDPA"
     gene            4933..>6606
                     /locus_tag="VD788_13430"
     CDS             4933..>6606
                     /locus_tag="VD788_13430"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXV77314.1"
                     /translation="MTSTVRHLLTMLLATAATIALVVAAGTALLRGSELDAAVARLQA
                     ETDLVARWVERIDPRTDCQDVATEIGRRLGVRVTLIAEEGTVLGDSSRTHASLARLDN
                     HLERPEIRGARRGAQGFSRRVSQTTNVEYVYTARTVSSPSPVYYVRLGLPAGELGPAT
                     RGNTWWWVVLALGATIPLGLVGYLSLRRLTRPVGQLADAVERTAGSELREPLPIPDHG
                     SDEVIRLGTNINRLKLTLLRTLDALDSERSTLSSVVAGMREGLLLVGADRRIRLANAA
                     LKRILDLDLEPEGRLLEAVVRHPSVVADIDSALSEREETQESVIKIPGSDRVFELHVT
                     PLEAARSGEHHDVLALFVDITRLDRLEKVRREFVANVSHELRTPLTSIKAFVENLIDG
                     GLEDGDNARKFLEIVRRHADHMGELIEDLTDLSLIETGSIMLELRRVDAAEVAREVVA
                     KCRPLAEARDVEVRIDLPSPLFVTSDRRRLEQMLTNLVHNALKFNREGGQVVIGGESS
                     LGATTLFVEDTGIGIPSNHLDKVFERFFQVNRDRSRAVGGTGLGLSIVKH"
BASE COUNT         1067 a         2197 c         2202 g         1140 t
ORIGIN      
        1 ggatccgacg agttcgagcc caagctgagc ctggtgcccc tcgccgtggg gacgctcaag
       61 ggcacgctgt actcgctcgt cctcgcgatc cccttgggcg tcctcggcgc gatgtatacc
      121 tcgcagttcc tgcaccaccg gctgcgccgg atcgtcaagc ccacggtcga gatcatggcc
      181 gcgctgccga gcgtggtgct cggattcctc gccggtctgt ggctcgcccc acgcgtcgaa
      241 cggctgatgc cggcgctggc gctgatgctg gtcgtcattc ccgtgctggt cgtcgccggg
      301 ggcgttcttt ggcgccgact gcccggcgcg gcgcgcagcc tgttgccgga cggcagcgag
      361 gcgatcgtgc aggtcggaat cgtcaccgcg gcagtcgcgc tctgcctgtg gctgaaccat
      421 ccgttcgagt ccgtcgtttt cgctggcgat ttccaacagt ggctgctcga cgtcaccggg
      481 gtcggctacg atcaacgcaa cgcggtgatt gtcggcttgg cgatgggctt cgcggtgatc
      541 ccgatcattt tctcgatcgc cgaggactcg ttcagcaacg ttccgcgaac gctggtctcg
      601 ggatcgctcg ccctcggcgc caaccgctgg cagacggtga cccgggtcgt cctcccgtcg
      661 gccagccccg gcatcttctc cgcgatcatg atcggattcg gccgcgcggt gggcgagacg
      721 atgatcgtgc tgatggccac cggcaataca ccgctgatgg actggaatcc attcaacggc
      781 tttcgcactc tctccgccaa catcgcggtc gagatccccg aagccccgca gagcggcacg
      841 ctctatcgca cgttgtttct cgccgcgttg ctgctgttcg ttttcacgtt cctgaccaac
      901 accgtcgccg aactcgttcg gcaacgcctg cgacagcgct acgcccagct atgagcgatg
      961 gtcgatgaag aagcgacggt cgggaggacg cgtggattgg ttgggcgacg ggttgacgtc
     1021 gctctgcgcc ggagcgctcg cgctcaacgt gttgctcgtc atcgcgttgc tctgcatcct
     1081 ggtcgtcaac ggcatgggct acttctggca gcggccgctc gtcgagatga cggtcgaaga
     1141 cggcatggtc tacctggggg agattcagga gcgcgagtcg attccggcga ccgacgatga
     1201 cggaaccgcg gcaggatccc gcgtgcggat caaggtcggc aaccgcgacc tgaccggagt
     1261 cgacttcgtc tggatcgacg agccgcgggt cgaatcgcgg cgaaccccgg acgacgcggt
     1321 gctgctcgag cgactggagt acggcaactt ctacggcttc ctcgagcggc tgcagcgcgg
     1381 cgacgaggtg ctggccgagg gctcggccga ggtgtgggag gcgttccaat ccctgcacgc
     1441 cgacaaactg cgcgaggcga cgctgattcg gcgtctcgag aagcgcgaga tcggcgatgt
     1501 caatcacgcg atggagcgcc tgcgcctggc cgagcgccga atcgaccggt ccgaactcga
     1561 cccgagcgag cgggagcagc ggctggctcg cctgagacag gaacaggcgc ttgccgaatc
     1621 ggagtacgag gcactcagtc gtcgtctgtt cgacatgcgc gagcgatggg cggccgagac
     1681 gctgatggtc cgtgcggtga acggcgcgct gcagcagatc ccggtggcga acgtggtccg
     1741 cgccgttcgg ccgaaccaac tcggcacgac cggtcgcctc gcgctctacc tcgagcgaat
     1801 ctgggagttc ctttcctccg acccgcgcga gtcgaacacc gagggcggga tcttccccgc
     1861 gatcttcggt acggtgatga tggtgttctt gatgtcgttc gccgtggcgc cgctgggtgt
     1921 gctggcggcg ctctacctgc gcgagtacgc caagcagggg ccgctggtgc gcaccgtccg
     1981 gatcgccgtc aacaacctgg ccggggttcc gtcgatcgtc ttcggcgtgt tcggtctcgg
     2041 tttcttcgtc tatctcgtcg gtggttcgat cgaccgactg ttctacccgg agtcgctgcc
     2101 cacgccgacg ttcggcaccg gtggtatcct ctgggcgagc ttgacgctcg cgttgctgac
     2161 cgttccggtg gtcatcgtcg ccaccgagga gggacttgcc gcagtgccgc gtatcgtccg
     2221 cgagggttcg ctcgccctgg gcgcgaccaa gttcgagacg acctggcgcg tggtggtccc
     2281 cgctgcggca ccgggcatcc tcaccggcct gatcctggcg atggcgcgcg cggcgggcga
     2341 ggtcgctccg ttgatgattg tcggcatggt caagctcgcc ccggcgttgc cggtcgacca
     2401 ccacttcccg ttcgtccatt tcgaacgcaa gttcatgcac ctcgggttcc acatctacga
     2461 cgtcgggttt cagagcccga acgtcgaggc gaccaagccg ttggtgttcg ccacggcgct
     2521 gctgttgatt ctggtcgtga ccgcgatgaa cctgttggcg atcggcgcgc gaaaccacct
     2581 gcgccgcaag tacatgggaa gcgcggtata gcgatgaagg gatcgtcgtg acgggcgaac
     2641 tgcgtgccga gaagttcggc tccgacgaca cgcgcgctcc ggccgaccgt ccggctggcg
     2701 gtcgtggccc ggacgccggt cgcccctccc gaaccgatcc ggccgccccc ggcggcgacg
     2761 aacgcacggc gatcacgccc gacatcgccg acccgatcct cgacatccgc gcgctcagcc
     2821 tgtggtacgg ctcgaaccag gcgctgcacg acatctcgct gctgatcggc cagcgcaaga
     2881 tcaccgcgtt catcgggccg tccggctgcg gaaagtcgac cttgctgcgc tgtctcaatc
     2941 gtctcaacga catgatcgac gaggtgcggg tcgaaggcca gatcctgttc gagagcgaag
     3001 acattcgcaa cccggccgcc gacgtcaacg cgctgcgcac gcggatcggc atggtctttc
     3061 agaagtccaa tccgttcccg aaatcgatct acgacaacgt ggcctacggg ccccgcatcc
     3121 acggcatccg cgaccgccgc cagctcgacg agatcgtcga gcgcagtctg cggggcgcgg
     3181 cgttatggga tgaggtcaag gaccgactca agcacaccgc cctcggtctg tccggcggac
     3241 aacagcagcg gttgtgcatc gcccgtgcga tcgcgatcga gccggaagtg atcctgctcg
     3301 acgaaccgtg ctcggcgctc gatccgatcg cgacggcgaa gatcgaggaa ctgatgatcg
     3361 agctcaagga gcggtacacg ttggtgatcg tgacgcacaa catgcagcag gcgtcgcgcg
     3421 tgtcggactt caccgccttc atgtacctcg gccgtctggt cgagtaccaa ccgaccgacc
     3481 ggctgttcat caacccgatc cgcaaacaga ccgaggaata catcaccggt cgcttcggct
     3541 aggccggcaa cgagcatccg atggaccaat ccgcagttca gctacgcgag cagctcgacc
     3601 tgttgtcgga attgatactg cgactcggcg gaatggccga ggacgcgatc ggcaagtcgg
     3661 tccgggcgct cgtcgaccgc gattccgacg tcgggcggga ggtgatcgag ggcgacaagg
     3721 aggtcgatgc gctcgagctc gagatcgacg gcttgtgcat gtcgattctg gcgcgccacc
     3781 agccgatggc gggcgatctt cgcttcgtcg ccacggcgat gaagatcacg cccgatatcg
     3841 agcggatcgc cgaccattcg gtcaacatca gcgagcgcgc gctcgagctg aacgacgagc
     3901 cgccgctcga cgcgctgatc gacctgccaa tgatggccgc gcgcgtacag cagatggtgc
     3961 gcggcgcgct cgacgcgttc gtgcgccgcg acgccaaggt ggcgctcgac gtgatccgga
     4021 tggacgccga ggtcaaccgg cggatggaac aggtgtttcg tgtgctgttg tcctacatgc
     4081 tgtccgaccc gacgacgatc agtcgcgcgt tgcgtacgac cttcgtcgcg aaatacttcg
     4141 agcggatcgg cgaccaagcg accaatatct gcgagcagat cgtctacatg accgacgcca
     4201 gggtcatcaa gcaccaagcg tttcccaacg actagcatcc cggatgaaac ggatcgtcat
     4261 catcgaagac gacgccgaca tcgcgctgtc gatcaagtac tcgatggaac gtgagggcga
     4321 gttcgcggtg accgtcgccc gcgacggcga ggccggcatc gaggctgcca cgcgatcggt
     4381 gccggatctc gtcctgctcg acctcaacct tcccggtctc gacggcaccg aggtctgccg
     4441 cgtcttgcgc tcgaaccgca gcacgtccgc ggtgccgatc atcatgctga ccgcccgcgt
     4501 cgaggagcgc gagaaggtga ccgggctcga cctcggagcg gatgactaca tcaccaagcc
     4561 gttctcgata agggaggtgc tggcccgcgt gcgcgccgtg ctgcgccgca gcgccggcgg
     4621 gtcgcgcggt cacaagccgc tcgctttcgg gccgctgacg ctcgacgaag cccgccggcg
     4681 cgtcaccgtc gacggcgagg agatccggtt gacccgcaag gagttcgatc tgctggccga
     4741 cctgatgcgc cgaccggacc tggtgttgac gcgcgagttg ctgctggccc gcgtctgggg
     4801 ttacgatcat ccgggagcga ctcgcaccgt cgacgtccat atccggcagc tgcgcaagaa
     4861 actgggtcag gccgccgccc gctacatcga aacggtcgtc ggcgtcggct accggttccg
     4921 gggagatcct gcttgacctc caccgttcgc cacctgctga cgatgttgtt ggctaccgcg
     4981 gccacgatcg cgttggtggt ggcggccggc acggccctgc tgcgcgggtc cgagctcgac
     5041 gccgccgtcg cccggctgca ggccgagacc gacctcgtgg cacgctgggt cgaacggatc
     5101 gacccccgca ccgattgcca ggacgtggcg accgagatcg gccgacggct cggcgtgcgc
     5161 gtcacgctga tcgccgagga gggcaccgta ctcggcgact cgtcgcgcac gcacgccagc
     5221 ctggcccggc tggacaacca cctcgagcga cccgagatcc gcggcgcccg ccgcggcgcc
     5281 cagggcttca gccgccgcgt ctcccagacg accaacgtcg agtacgtcta caccgcacgc
     5341 accgtgtcga gccccagccc ggtgtactac gtccgactcg gacttcccgc gggcgagctg
     5401 ggacccgcca cgcgcggcaa tacctggtgg tgggtcgtgc tcgccctcgg cgcgacgatt
     5461 ccgctgggct tggtcgggta tctgtcgttg cgccggctga ctcgaccggt cgggcagctg
     5521 gccgacgcgg tcgagcgaac ggccggcagc gaactgcgcg agccactgcc gatcccggat
     5581 cacggcagcg acgaggtgat ccggctcggc accaacatca accggctgaa actgacgctg
     5641 ctgcgaacgc tcgacgcgtt ggactcggaa cgctcgacgc tgtcgtcggt ggtggcgggc
     5701 atgcgcgagg ggttgctgct ggtcggtgcg gatcgccgga tccgcctggc gaacgccgca
     5761 ctgaagcgga tcctcgacct cgacctcgaa cccgagggtc gactgctcga agccgtggtc
     5821 cgccatccgt cggtcgttgc cgacatcgac tccgcgctgt ccgagcggga ggaaacgcag
     5881 gaatccgtca tcaagatccc cggatcggac cgggtcttcg agttgcacgt caccccgctc
     5941 gaggccgcga ggagcggcga gcatcacgac gtgctggcgc tgttcgtcga cattacgcgc
     6001 ctcgatcggc tggaaaaggt gcggcgcgag ttcgtcgcca acgtctccca cgaactgcgc
     6061 acgccactga cctcgatcaa ggccttcgtc gagaatctga tcgacggtgg tctggaggac
     6121 ggcgacaacg cgcgcaagtt cctcgagatc gtgcggcgcc acgccgacca catgggcgag
     6181 ctgatcgagg acctgaccga cctgtcgctg atcgagaccg gctcgatcat gctcgagctg
     6241 cgccgcgtcg atgcggcgga ggtcgcgcgc gaagtggtgg cgaagtgtcg gccgctggcc
     6301 gaggcgcgcg acgtcgaggt gcggatcgac ctgcccagcc cgctgttcgt gacctccgac
     6361 cgccggcgcc tcgagcagat gctgaccaac ctggtccaca acgcgctcaa gttcaatcgc
     6421 gagggcggcc aggtggtgat cggcggtgag tcatcgctcg gcgcgacgac gctgttcgtc
     6481 gaggacaccg gaatcggcat cccgtcgaac cacctggaca aggtgttcga gcggttcttc
     6541 caggtcaatc gcgatcgttc gcgcgccgtc ggcgggacgg gcctcggctt gtcaatcgtc
     6601 aagcac
//
LOCUS       DASPGN010000460         4966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1138945, whole genome shotgun sequence.
ACCESSION   DASPGN010000460 DASPGN010000000
VERSION     DASPGN010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1138945"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2061
                     /locus_tag="VD788_13435"
     CDS             <1..2061
                     /locus_tag="VD788_13435"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXV77315.1"
                     /translation="KLYRIRYPAAGGGAGVEVATTRPETLLGDTAVAVHPADERYRKI
                     VGGKVEVPLTGRSVPVIADEFVDREFGTGAVKITPAHDPNDFDAGLRHRLPQLAVIDT
                     TAKMTPAAGRFAGLDRFEARRQVVDALEQQGLLVDTRPHEHAVGHCQRCGTMIEPLVS
                     TQWFVKIAPLAQPAIEAVERGATRFVPDNWSKTYFEWMRNIHDWCISRQLWWGHRIPA
                     WYCDRCDETIVALESPAECPECRGALRQDEDVLDTWFSSALWPFSTLGWPEQSAELKR
                     YYPTSVLITGHDIIFFWVARMMMMGLHFIDLVPFREVYIHGLVRDAHGQKMSKSKGNT
                     VDPVELQARYGTDAVRFTMAILAAPGNDIPLAHERMEGYRAFANKLWNASRFVLLKLG
                     DRRPAMPDRERLGLVDRWILSRLRATIQDVDRALGEYRFDRAADRLYHMIWHEFCDWY
                     IELAKPAFDGTDAEREETASAVLLEVLATLLRLLHPMMPYVSEELWQRIHRDGSFLAV
                     ARWPEADDRDDDAQAERDVELLQEVVTRVRNMRAESNIAPGQRVRLMLHADDAGTADM
                     LRAQAVLIGTLVRAERVEIVDGLDRQTVAARGLARGVQIAIPLAGVLDLAAERGRLLR
                     ELAKLEREIDARRRKLDNPSFREKAPADVVDKERRIQQEVVERFDRLQGLLAGLGSDS
                     AGGG"
     gene            2068..2916
                     /gene="nadC"
                     /locus_tag="VD788_13440"
     CDS             2068..2916
                     /gene="nadC"
                     /locus_tag="VD788_13440"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HXV77316.1"
                     /translation="MRLERELLIPIVERALEEDLGRAGDLTGDAVVPVAARGEAVIVA
                     RQSLILAGIPLAREVYRAIDPELRFSAAHEDGSRVDRDTVVARVAGSTRALVRGERTA
                     LNFLMRMSGIATATRAAVDEIDGTSARILDTRKTAPGLRLLDKYAVRCGGGLNHRMGL
                     YDAVLIKDTHLGAGCTVGQAVRRALDAGHPAAIVTAEVRGLDQLEQAIEAGVGRVLLD
                     NMPLEMMAEAVRRAERRVVIEASGGLRPGHLRSVAETGVDFLSLGWLTHSAPAADLAM
                     EIREPR"
     gene            2958..3749
                     /locus_tag="VD788_13445"
     CDS             2958..3749
                     /locus_tag="VD788_13445"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HXV77317.1"
                     /translation="MGNLLRGTPFDGKVEVRGTVGSTNDDLRVLAALGAPQWTVVVAE
                     RQTAGRGRHGRRWHSAAGSGLYLSVLLRPRQPLEHVGRHTLAVSVAIAEACRSSCGVE
                     VRIKWPNDLIHDGLKLGGVLAELRSSTRYGPELVVGTGVNVDLSRAEIPPELAGIATS
                     LRIASGRSMLDRERLAGEYLLRLERIATLMDGEGWPEVRRRWLALSPGANGDRVRVTS
                     DHAGGSEQFDGRTAGIDDSGALVVRRSDGALVTVSSPHSVSPLGE"
     gene            3752..4519
                     /locus_tag="VD788_13450"
     CDS             3752..4519
                     /locus_tag="VD788_13450"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HXV77318.1"
                     /translation="MLLAVDIGNTHTVLGLFDGETLRADWRVGTRRDATADEIAVLLG
                     ALFREAGIERAQVGGTIVSSVVPDLDAPYRDAVARYLDRAALFVGPGVKTGMPILYEN
                     PHQVGADRIVNSVAAMDRYGAPVIVLDFGTATTFDVVGAEGQYLGGVIAPGLGASAEA
                     LFAKAARLPHVDLRRPGRVIGRNTENSIQSGLYFGYAALVEGLLERIRRELDADAPVI
                     ATGGLAVVLEPELPFLTATLPDLTLHGLRLIWQRNRP"
     gene            4516..>4966
                     /locus_tag="VD788_13455"
     CDS             4516..>4966
                     /locus_tag="VD788_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77319.1"
                     /translation="MKVVFVCSGNICRSPMAAEYFRHRAKSEGLTDCRVASGGTLGID
                     GAPASSEAIEAMAEIGLDLSRHRSRALSAETLSGADYTIAMEQDHLEYLARRHPEGDD
                     ARMLLRAFEDDARPRPSAHDLPDPMGNPLSFYREQLPLMTRCVDHLIT"
BASE COUNT          753 a         1650 c         1752 g          811 t
ORIGIN      
        1 aagctctacc gaatccgcta tccggccgcc ggcggcggcg cgggcgtcga agttgcgacg
       61 acccgacccg agacgttgct cggggacacg gcggtcgcgg tccatccggc ggacgagcgc
      121 taccggaaga tcgtcggcgg aaaggtcgag gtcccgctca ccgggcgcag cgtgccggtc
      181 atcgccgacg agttcgtcga tcgcgagttc ggtacgggag cggtcaagat cacgccggct
      241 cacgacccca acgacttcga tgccggcctg cgtcatcgtc tcccgcaact ggcggtgatc
      301 gatacgacgg cgaagatgac ccccgcggcc gggcgtttcg ccgggctcga tcggttcgaa
      361 gcgcgacgcc aggtcgtcga cgctctcgag cagcagggcc tgctggtcga tactcggccg
      421 cacgagcacg ccgtcggcca ctgccaacgc tgcggaacga tgatcgagcc gttggtgtcg
      481 acccagtggt tcgtcaagat cgccccgctg gcccagccgg cgatcgaggc ggtcgagcgc
      541 ggagccactc gtttcgtccc ggacaactgg tccaagacct acttcgagtg gatgcggaac
      601 atccacgact ggtgcatctc gcgccaactg tggtggggtc accgcatccc ggcctggtac
      661 tgcgaccgtt gcgacgagac gatcgtcgcg ctggaatcgc cggccgagtg tccggagtgc
      721 cgcggcgcgt tgcgccagga cgaggacgtc ctcgacacct ggttctcctc cgcgctctgg
      781 ccgttctcca cgctgggctg gccggagcag agcgccgagc tgaagcgcta ctacccgacc
      841 agcgtgctga tcaccggtca cgacatcatc ttcttctggg tcgcgcggat gatgatgatg
      901 ggtctccact tcatcgatct cgtcccgttc cgcgaggtct acatccacgg gctcgtgcgc
      961 gacgcgcacg gccagaaaat gtcgaagtcc aagggaaata cggtcgatcc cgtcgaactg
     1021 caggctcgct acggcaccga cgcggtccgg ttcacgatgg cgattctcgc cgcccccggc
     1081 aatgacattc cgttggctca cgaacggatg gaaggctatc gcgctttcgc caacaagctg
     1141 tggaatgcat cgcgcttcgt gctgctcaag ctcggcgacc gcagacccgc gatgccggac
     1201 cgcgagcggc tcggcctggt cgatcgctgg atcctgtcgc gtctgcgtgc cacgatccag
     1261 gacgtcgacc gcgctctggg cgagtaccgc ttcgaccgcg cagccgatcg tctgtaccac
     1321 atgatctggc acgaattctg cgactggtac atcgagctgg ccaagccggc attcgatggt
     1381 acggacgccg aacgcgagga gacggcgtcc gcggtcttgc tcgaggtatt ggccacgctg
     1441 ttgcgcctgc tgcacccgat gatgccctac gtcagcgagg agctgtggca gagaatccat
     1501 cgtgacgggt cgtttctggc cgtcgcccgt tggccggagg ccgacgaccg ggacgatgac
     1561 gcgcaagccg agcgcgacgt ggagctgttg caggaggtcg tcactcgggt tcgcaacatg
     1621 cgtgccgaat cgaacatcgc accgggccag cgggttcggt tgatgctgca tgccgacgac
     1681 gccggcacgg ccgacatgct gcgcgcgcaa gccgtcctga tcggtacgtt ggtccgcgcc
     1741 gagcgggtcg agatcgtcga cggactggat cggcagaccg tcgcggcgcg cgggcttgcg
     1801 cgtggcgtcc agatcgcgat cccgctcgcc ggggttctcg acctggcggc cgagcgcggc
     1861 cggctcctgc gcgagttggc caagctcgag cgcgagatcg acgcccgccg gcgcaagctc
     1921 gacaatccgt cgttccgcga gaaggctccg gccgacgtcg tcgacaagga gcggaggatc
     1981 cagcaggagg tcgtcgagcg tttcgaccga ctgcagggtc tgctcgcggg actcggcagc
     2041 gacagcgccg gcggcgggtg atcgcccgtg cggctggagc gagagctctt gattccgatc
     2101 gtcgagcggg ccctcgagga ggatctggga cgcgccggcg atctcaccgg tgatgcggtc
     2161 gtcccggtcg ccgcacgagg cgaggccgtc atcgtcgcgc gtcagtcgct gatccttgcc
     2221 ggtattccgc tcgcccgcga ggtgtatcgc gcgatcgacc ccgagctgag attctccgcc
     2281 gcgcacgagg acgggtcgcg cgtcgatcgc gacaccgtgg tcgcgcgggt cgcgggttcg
     2341 acgcgcgcgc tcgtgcgagg cgagcggacg gcgctgaact tcctgatgcg gatgagcggg
     2401 atcgccaccg ccactcgcgc cgcggtcgac gagatcgacg gcacctcggc caggatcctc
     2461 gatacccgca agaccgctcc cggtctgcgc ttgctcgaca agtacgcggt ccgctgcggc
     2521 ggggggctga accaccggat ggggctctac gacgcggtgc tgatcaaaga cacgcacctc
     2581 ggtgccggtt gcacggtcgg gcaggcggtg cgtcgcgcgc tcgacgcggg gcatcccgcc
     2641 gcgatcgtca cggcggaggt ccgcggtctc gaccaactcg agcaggcgat cgaggccggg
     2701 gtggggcgcg tgctgttgga caacatgccg ctcgagatga tggccgaagc cgtccgtcgc
     2761 gccgagcggc gggtggtcat cgaggcgtcg ggcgggctga gaccggggca cttgcgttcc
     2821 gttgccgaaa ccggggtcga cttcctcagc ctgggctggt tgacccattc cgcacctgcc
     2881 gccgacctgg cgatggaaat ccgggagcca cgctgaaccg ccccgctcga tctcgcgagc
     2941 cgatcgatcc ccttcgcttg ggcaaccttc tccgcggcac gcccttcgac ggcaaggtcg
     3001 aagtgcgggg gacggtcggc tcgaccaacg acgacctgcg ggtgctggcc gccctcggcg
     3061 ccccgcaatg gacggtcgtc gtcgcggagc gacaaaccgc gggtcgcgga cgtcacgggc
     3121 gccgttggca ctccgccgcc ggcagtggac tctatctgtc cgtgctgctc cgtccacgcc
     3181 agcccctcga gcacgtcggg cgccatacgc tggccgtttc ggtcgcgatc gccgaagcgt
     3241 gtcgttcgag ctgcggcgtc gaggttcgga tcaagtggcc caacgacttg atccatgacg
     3301 ggctcaaact gggaggcgtg ctggccgagc tgcggagctc gacccgctat gggcccgagc
     3361 tcgtcgtcgg caccggagtc aacgtcgacc tgagccgagc cgagataccg ccggagctgg
     3421 ccgggatcgc gacgtcgttg cgcatcgcca gcggccgtag catgctggat cgtgagcgct
     3481 tggccggcga gtacttgctc cgtctcgagc ggatcgccac tttgatggac ggcgagggct
     3541 ggccggaggt gcggcgtcga tggctcgcgc tgtcaccggg ggcgaacgga gaccgcgtgc
     3601 gcgtcacgtc cgaccacgcc ggcgggtcgg agcagttcga cgggcggact gccgggatcg
     3661 acgacagcgg cgcgttggtc gtccgacggt cggacggggc gctggtgacg gtgtccagcc
     3721 cgcattcggt ctcgccgctc ggagagtgac gatgctgctg gcagtcgaca tcggcaacac
     3781 acacaccgtg ctcgggctgt tcgacggcga gacgctccga gcggactggc gtgtcggcac
     3841 gcggagggac gccaccgcgg acgagatcgc ggtgctgctc ggcgcgttgt ttcgcgaggc
     3901 cgggatcgag cgagcgcagg tgggtggtac gatcgtctcc tcggtcgtcc ccgacctcga
     3961 cgcgccctat cgcgacgcgg tcgcgcgata cctcgaccgc gcggcgctgt tcgtcgggcc
     4021 tggcgtcaag acggggatgc cgatcctcta cgagaatcct caccaggtgg gagccgatcg
     4081 gatcgtcaac tccgtcgcgg cgatggaccg ttacggtgcg ccggtgatcg tgctggattt
     4141 cggcaccgcg acgaccttcg acgtggtcgg ggcggagggg cagtacctcg gcggcgtgat
     4201 cgcgccgggg ctcggagcgt cggcggaggc gttgttcgcc aaagcggcac ggctgccgca
     4261 tgtcgacctg cgccgccccg ggcgggtcat cggccgcaac accgagaaca gcatccagtc
     4321 gggtctctac ttcggctatg ccgcgctggt cgagggcttg ctggaacgga tccgccgtga
     4381 gttggacgcc gatgcgccgg tcatcgcgac cggtggtctg gccgtcgtgc tcgaacccga
     4441 gctgccgttc ctcacggcga cgctcccgga tctcacgctc cacggcctgc gcctgatttg
     4501 gcagaggaac cgtccgtgaa ggtggtgttc gtctgcagcg gcaatatctg ccgcagtccg
     4561 atggccgccg agtacttccg tcatcgggcg aagagcgaag gtctgaccga ctgccgcgtc
     4621 gcgtcgggag gaacgctcgg catcgacgga gcgcccgcct cgtcggaggc gatcgaggcg
     4681 atggcggaga tcggcctgga tctttcgcgg catcgctcgc gggcattgag cgccgagacc
     4741 ttgagcggcg ccgactacac gatcgcgatg gagcaggacc acctcgagta cctggctcga
     4801 cgccatcccg agggcgatga tgctcgcatg ctgctgcggg cattcgaaga cgacgctcgc
     4861 ccgcgcccga gcgcgcacga tctgcccgac ccgatgggca acccgctgtc gttctaccgc
     4921 gagcaactac cgctgatgac gcgctgcgtc gaccacctga tcacct
//
LOCUS       DASPGN010000461         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1020880, whole genome shotgun sequence.
ACCESSION   DASPGN010000461 DASPGN010000000
VERSION     DASPGN010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020880"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1580
                     /locus_tag="VD788_13460"
     CDS             <1..1580
                     /locus_tag="VD788_13460"
                     /inference="COORDINATES: protein motif:HMM:NF023048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HXV77320.1"
                     /translation="PVLLELLRDPDCRRRDNVVAFLTYLAADEHVPPLVQLLEFPPAD
                     PKIPEEDRSLLLVLDALGKIAARGGVLALAELRRLAEAGSTDDAPGRAVARGAYDAEL
                     RDQIRARAVTRLRTAGNDDPRGTGGGRQRPVRLSDVEPPVSEGGAAVGRLVDGNPRAE
                     DAAITYVNHVDLINRLDDATLDFALSSASFNAGSVDFDQDVGCCVTLTRQGPGGTFGS
                     PGDGLDTLDTDQQVRAALDADSARVKVVRLINHCGLPGTNIIGCSYLPGPAMAVVRLT
                     SPADEGLLWLHEFGHNVGLDHNPDSRFVMHGVLNGRNTGINQTECNRYHAPASSAAVV
                     QTDLGVCHDDDDDDLASSIDNCPSQANTSQTDGDADGVGSACDNCAVDANADQADGDG
                     DGIGDVCDLCRDLDGDGFGEPADADCPAGPIADCDDARSDVSPAALELCDGVDNDCDL
                     GVDDLTCDGFDATGDLRVDGHELAWVGRAFGSCSATPQDLWWAPVDYSRDGCVDGDDL
                     MLLGVVWACTGSNPVCD"
     gene            1630..>2725
                     /locus_tag="VD788_13465"
     CDS             1630..>2725
                     /locus_tag="VD788_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77321.1"
                     /translation="MATGTTAVRRAPGVWVHLGLVSLAALPYLGMLSNPLVYDAPETI
                     TRNEAVQHGRLGDLLRVDFWGDPLDADFSTRSYRPLVSLTFALQVRAGAGARALHLAD
                     LALHALSALLVMRLIERLGVARRWAFAGAAVFAVHPVQSEAVASVVGRADLMAGACLL
                     GALLFALGGRDRGRRPLDEAGALTLLGAALFCKEYAVVFPFVLIAVDLVAGPHDRPRR
                     FRLWAAALGWVAGYLLLRRAMIGDVGGVASLAATDHPLFDAPLSTRVAMALRLLALVV
                     RLLVWPTALNHHYRVGTLEIVDGLLDPLVLAGASWVMLSGWFAWRRLARGQPIPTVAF
                     VLLWWPLLPSLNLVSLAGVVFAERFLYVPLA"
BASE COUNT          337 a          970 c          969 g          449 t
ORIGIN      
        1 tgcccgtgct gctcgagttg ctgcgcgacc ccgactgccg tcgccgggac aacgtcgtcg
       61 cctttctcac ctatctggcc gcggacgaac acgtgccgcc gctcgtccag ctgctcgagt
      121 tcccgccggc cgatccgaag atcccggagg aggatcgcag cctgctgctg gtgctggacg
      181 cgctgggaaa gatcgccgcc cgcggcggcg tgcttgcgct ggccgagctg cggcgcttgg
      241 ccgaggcggg ttcgaccgac gatgcgccgg gccgggccgt ggcgcgcggt gcgtacgacg
      301 cagagctgcg tgaccagatc cgcgctcgcg ccgtgacgcg gcttaggacg gcggggaacg
      361 acgacccgcg aggcaccggc ggggggcgtc agcgacccgt ccggttgtcg gacgtcgaac
      421 cgccggtgag cgagggcggt gcggccgtcg gtcggctcgt cgacggcaac ccgcgcgcgg
      481 aagatgccgc gatcacctac gtcaaccacg tcgacttgat caatcggctc gatgacgcga
      541 cgctcgactt cgcgctgtcg tccgcttcgt tcaacgccgg gtcggtcgac ttcgatcagg
      601 acgtcggctg ctgcgtcacg ctgacgcggc agggtccggg cgggacgttc ggcagtccgg
      661 gggacggtct cgacacgctc gacaccgacc aacaggtgcg cgccgcgctc gacgccgatt
      721 ccgcccgggt caaggtcgtc cggttgatca atcactgcgg gttgccggga accaacatca
      781 tcggctgctc gtacctgccc ggtccggcga tggccgtcgt acggctgacc tcccccgccg
      841 acgaggggct gctctggctg cacgagttcg gccacaacgt cggcctcgac cacaacccgg
      901 actcgcgctt cgtcatgcac ggcgtactca atggccgcaa caccgggatc aaccagaccg
      961 agtgcaaccg ttaccatgcg ccggcctcgt cggccgccgt cgtgcaaacc gacctcggcg
     1021 tctgtcacga tgacgacgac gacgatctcg ccagctcgat cgacaactgc ccgtcccagg
     1081 cgaacacgtc ccagaccgac ggcgacgccg acggcgtggg cagcgcctgc gacaactgcg
     1141 cggtcgacgc caacgccgac caggcggacg gcgacggcga cgggatcggc gacgtgtgcg
     1201 acctctgccg ggatctcgac ggagacgggt tcggcgagcc ggcggacgcg gactgccccg
     1261 ccggtccgat cgccgactgc gacgacgcgc gctcggacgt ctcccccgcg gcgctcgagc
     1321 tttgcgatgg tgtcgacaac gactgcgacc tgggcgtcga cgacctgacg tgcgacgggt
     1381 tcgacgcgac gggcgatctg cgcgtcgacg gccacgagct ggcctgggtc gggcgggcgt
     1441 tcggcagttg cagcgcgacg ccgcaggacc tgtggtgggc gccggtcgac tacagcaggg
     1501 acggctgcgt cgacggggac gacctcatgc tgctgggcgt ggtttgggcc tgcaccggct
     1561 cgaatccggt ctgcgactga gacgccgggc gattccctat aatcggcgct tgccgatgac
     1621 gattccagcg tggcgacggg aacgaccgcg gtgaggcgtg cgccgggagt ctgggtccac
     1681 ctcgggctcg tgtcgctcgc ggctctccct tacctgggca tgctgtcgaa cccgttggtc
     1741 tacgacgcgc ccgaaacgat cacgcgcaac gaagcggtcc agcacgggcg gctcggcgac
     1801 ttgctgcggg tcgatttctg gggcgacccg ctcgatgccg acttctccac gcgttcctac
     1861 cgcccgctgg tcagcctgac cttcgcgctg caggtgcgcg ccggggcggg cgcgcgcgcg
     1921 ctgcatctcg ccgacctcgc cctgcacgcg ctgtcggccc tgctggtgat gcggctgatc
     1981 gagcggctgg gcgtcgcgcg gcgctgggca ttcgccggcg cggcggtttt cgccgtgcac
     2041 cccgtccaga gcgaggccgt cgccagcgtg gtcggccgcg ccgatctgat ggccggcgcc
     2101 tgcctgctcg gggcgctgtt gttcgcgctg ggcggtcgcg atcggggccg ccgcccgctc
     2161 gacgaggccg gcgcactgac gctgctcggc gccgcgctct tctgcaagga gtatgcggtc
     2221 gtctttccgt tcgttctgat cgccgtcgac ctcgtcgccg gtccgcacga ccggccccgc
     2281 cgctttcggc tgtgggccgc ggcgctaggc tgggtcgccg gctatctgct gctgcgccgg
     2341 gcaatgatcg gcgacgtcgg cggggtcgcg tccctcgcgg caacggatca cccgctgttc
     2401 gacgcgccgc tgtccacgcg cgtcgcgatg gcgctgcggt tgctcgcgct cgtcgtccgt
     2461 ctgctcgtct ggccgaccgc gctcaaccac cactatcggg tcgggacgct cgagatcgtc
     2521 gacggcctgc tcgacccact ggtgctggcc ggggcgagtt gggtgatgct gagcggctgg
     2581 ttcgcctggc gccgcctcgc tcgcggtcag ccgatcccca ccgtcgcgtt cgttctgctg
     2641 tggtggccgc tgctgccgtc gctcaacctc gtgagcctcg ccggcgtggt cttcgccgag
     2701 cgcttcctct acgttccgct ggccg
//
LOCUS       DASPGN010000462         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_85956, whole genome shotgun sequence.
ACCESSION   DASPGN010000462 DASPGN010000000
VERSION     DASPGN010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85956"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VD788_13470"
     CDS             complement(<1..700)
                     /locus_tag="VD788_13470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXV77322.1"
                     /translation="MRVASWNVNGLRARIDLVLRWLVERRPDVVGLQELKLTDDQFPH
                     DRFLEHGYRAAVHGQKAWNGVAVLSRDEAEVVQRGLPGQTAAGARMLEVRSGALRFAT
                     VYVPNGKSVGHTDFATKLSWLDALAGWLRDGGRTAEPFVLCGDFNLCPAPLDSWNEAL
                     LAGSIFHTDDERSRFRALLDCGLHDLFRELEPQAQAFSWWDYRGGAFHRKQGLRIDFL
                     LASGTIRQRLRSVAG"
     gene            797..1498
                     /locus_tag="VD788_13475"
     CDS             797..1498
                     /locus_tag="VD788_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77323.1"
                     /translation="MAKKTGGKSRAKRTAATSGGGAKFAGVVELTGPGDDDRAAIANG
                     TRVVVSLVEVRVGRLPEQGARAELQLDSRVRTTTADGEALTVGWSPGLFYYVLPESRL
                     NIDDTVIFDGEVYQHLSLDLELVERELPQFDPADAADLAAAAAAATAELVGASGRIGS
                     ALDAFPSILGGILKLNGDDQVLKYATSLYTREVNLAAGSGRRLLEGRYRIDKRRSPGD
                     ADSAIGLVLDVRGVA"
     gene            1601..2011
                     /locus_tag="VD788_13480"
     CDS             1601..2011
                     /locus_tag="VD788_13480"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXV77324.1"
                     /translation="MRKSNPGPSVVGLMVLIPTVAFVVWAAEIPESVTIDECQAKQSA
                     VVFPHKQHVDHGMACVTCHHTQADLKADSGVAVEKCGSCHLEPAEATTPKCSEMSTTK
                     NPYHIGCIDCHKQEAQKNEATKAPTKCFDCHPKA"
     gene            2102..>2978
                     /locus_tag="VD788_13485"
     CDS             2102..>2978
                     /locus_tag="VD788_13485"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXV77325.1"
                     /translation="MVRRSFGVRRVALCAVDFPNLGDLIALLPLLDGVRRLHPACSIL
                     LAGRFERLQLAERLGFTDRTLLYREAGFGLWREIRRFRPDLSLCLRRTSLRANLCFGR
                     PSGARLTVGFPGGVNRLVHTRLAPYRPLVDRPRRDAAPLDLLGGDCDLVATVRRVAAA
                     GRLVPPTEPYGLIVPGGAIDEKLWGAERFAAAAAGVTRQARELGWQVVLGPLEAERGY
                     PAVFARWLPAAQLVAGLELPDLARLVLGAGLVLANDCGPAHLAQMGGVPLVQLFGNWD
                     GRAGRRIDWWFDRRPG"
BASE COUNT          424 a         1058 c         1028 g          468 t
ORIGIN      
        1 cgcccgcgac cgagcggaga cgctggcgga tcgtgccgct cgccagcagg aaatcgatgc
       61 gcaggccctg cttgcggtgg aacgcccctc cgcggtagtc ccaccacgaa aaggcctgcg
      121 cctgcggctc cagctcgcgg aacaggtcgt gtagcccgca gtcgagcaac gcgcggaaac
      181 gcgaccgctc gtcgtcggtg tggaagatcg agccggccaa cagcgcctcg ttccaactgt
      241 cgagcggcgc cgggcagagg ttgaagtcgc cgcacagcac gaacggctcg gccgtccgcc
      301 cgccgtcacg gagccagccg gccagcgcgt cgagccagct gagcttggtc gcgaaatccg
      361 tgtgcccgac actcttgccg ttcggaacgt agaccgtcgc gaaccgcagc gcgccgctcc
      421 tgacctcgag catgcgggcg ccggccgcgg tctgtccggg caggccgcgc tgcacgacct
      481 cggcctcgtc ccggctgagc acggccacgc cgttccacgc cttttgcccg tggaccgccg
      541 cccgataccc gtgctcgaga aaccggtcgt gggggaactg gtcgtcggtc agtttcagct
      601 cctgcaggcc gacgacgtcc ggccggcgct cgacgagcca acgcaacacg agatcgatgc
      661 gcgccctcag cccgttgacg ttccagctgg cgacccgcac ctctcactcc caccccggcc
      721 cggctgctcg ctgcagacga taacacctct ccggcgcgcg gcgaccgcac gtacaatgat
      781 cggcggagga cgaccgatgg cgaagaaaac gggcggcaag tcccgggcca agcgcaccgc
      841 ggcgacgtcg ggtggcggtg ccaaattcgc cggcgtggtc gagctgaccg gacccggaga
      901 cgacgaccgc gcggcaatcg ccaacgggac acgcgtggtg gtcagcctgg tcgaggtgcg
      961 ggtcgggcgc ttgccggagc agggcgcgcg ggccgagctg caactcgact cgagggtgcg
     1021 cacgacgacg gccgacggcg aggcgctgac cgtcggctgg tcgccgggtc tgttctacta
     1081 cgtgctgccc gagtcgcgct tgaacatcga cgacaccgtc atcttcgacg gcgaggtcta
     1141 ccagcatctg tcgctcgatc tcgagctggt cgagcgcgag ctgccgcagt tcgacccggc
     1201 cgatgccgcc gatctcgcgg cggcggcggc ggcggcgacc gccgagctgg tcggagcgtc
     1261 gggccgtatc ggctcggcgc tcgacgcgtt cccgtcgatc ctcggcggga tcctcaagct
     1321 gaacggcgac gaccaggtgc tcaagtacgc gacctcgctg tatacgcgcg aggtcaacct
     1381 cgccgccggt tccggccggc gcctgctcga gggccgctat cgcatcgaca aacggcgctc
     1441 gcccggagac gccgacagcg ccatcggctt ggtgctcgac gtccgcggcg tggcgtaacg
     1501 cggcgattgc tgtcaaccgg ctctcgcgac cggttcggtc tgaattggcc tttcggggcc
     1561 ggcggagcag gtctaatatc ctgactggag gtgtgtaacc atgcggaagt cgaatccggg
     1621 tccgagcgtt gtcggtctga tggtcctgat cccgaccgtc gccttcgtcg tctgggcggc
     1681 ggaaataccc gagtcggtca cgatcgacga gtgccaggcc aagcagtccg ccgtcgtctt
     1741 cccgcacaag cagcacgtgg atcacggtat ggcgtgcgtg acctgccacc acacgcaggc
     1801 cgacctcaag gccgactccg gcgtcgcggt cgagaagtgc ggctcctgcc acctggagcc
     1861 ggcggaggcg accacgccga aatgctccga gatgagcacg acgaagaacc cctatcacat
     1921 cggttgcatc gactgtcaca agcaggaagc gcagaagaac gaggcgacga aagccccgac
     1981 caagtgcttc gactgtcacc ccaaggcctg atcgcgtcga tcgcaccggt ttccacccgc
     2041 ccgttttcgc ggtagcctcc ccggcacgtc cccggtcgac gggacgtgcc ggagtgccgc
     2101 tgtggtccgt cggtcgttcg gtgtcaggcg ggtcgcgctc tgcgcggtcg acttccccaa
     2161 cctgggtgac ctgatcgcgc tgttgccgct gctcgacggc gtgcggcggt tgcacccggc
     2221 gtgctcgatc ctgctggcgg ggcggttcga gcgactgcag ctcgccgaac gtctcggctt
     2281 caccgaccgc acgctgctct accgtgaggc gggcttcggg ttgtggcgcg agatccgccg
     2341 cttccggccc gacctctcgc tctgcctgcg gcggacgtca ctgcgggcca atctctgttt
     2401 cggccgcccc tcgggcgcgc gcttgaccgt cgggttcccc ggcggcgtca atcggttggt
     2461 ccacacgcgc ctcgccccgt accgaccgct ggtcgaccgg ccccgacgcg acgcggcacc
     2521 gctcgacctg ctcggcggtg attgtgacct ggtcgccacc gtccgtcgcg tcgccgccgc
     2581 cggtcggctc gtgcccccga ccgagcccta cggcctgatc gtcccgggcg gtgcgatcga
     2641 cgagaagttg tggggcgcgg aacggttcgc cgccgccgcc gccggtgtca ctcgtcaggc
     2701 tcgcgagttg ggttggcagg tcgtgctcgg cccgctcgag gcggaacgcg gctacccggc
     2761 ggtcttcgcc cggtggttac ccgcggcgca gctcgtcgca ggtctcgagc tgcccgacct
     2821 ggcccggctg gtgctcggcg cgggtctcgt gctggccaac gactgcggcc cggcgcatct
     2881 ggcgcagatg ggcggcgtgc cgctcgtaca gctcttcggc aactgggacg gtcgcgccgg
     2941 acggcggatc gattggtggt tcgaccggcg tcctggcg
//
LOCUS       DASPGN010000463         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_86655, whole genome shotgun sequence.
ACCESSION   DASPGN010000463 DASPGN010000000
VERSION     DASPGN010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86655"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="VD788_13490"
     CDS             complement(<1..1556)
                     /locus_tag="VD788_13490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HXV77326.1"
                     /translation="MAKVRVYQLAKDLNVQSALILELLDRMGQEVRSDLSTLDQKTAE
                     LVSMRIKSAIAAERQRVAVQERQQPMPPAEAAAPVASEPSAGQAEETPAPAEPAVHAP
                     AAEETAVPAAAAPADKPAARKSPARPESPAAAAAATSAIAGTTARKPRVFPARRVPPT
                     STLRPKPSPPPAARVAAGPTAPRPYGPPPGTVRRPAAAPVTPGKKRRKKEKKERQAVV
                     VPTARVPKELPPVPAEITLSEGVTVKELAEKLNRKSKDVIAKLIPRGILATINQPLEP
                     DVAIEVAKEFGAEARIITFEEEAQQVATPDLLGETEVADDTPDQLVPRPPVVTVMGHV
                     DHGKTSLLDAVRETSVVDSEAGGITQHIGAYQVEARNRKITFLDTPGHEAFTMMRARG
                     AKATDIVVLVVAADDGVKPQTLEAIDHARAADVPIVVAINKMDKPGANPARVKQQLAD
                     RNLLVEEYGGDIVSCEVSAKKRTGIDELLEMILLVADLKDLKANPNKPATGVVLEARL
                     DRARGVLATV"
     gene            complement(1578..3050)
                     /gene="nusA"
                     /locus_tag="VD788_13495"
     CDS             complement(1578..3050)
                     /gene="nusA"
                     /locus_tag="VD788_13495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXV77327.1"
                     /translation="MSSELYHTIEQIGREKGLDTETVIQAIEEAYAAATRKYYRSKKD
                     FGARFDRATGDFAVFSKQVVVEEDELVDPQTEISLDEAKLTRPDVELGMVIETPYDTA
                     EAPLTRIAAQAAKQVIYQKVKEAERELVWHEYADRVGELLTGQVKRFDRGDMILDLGR
                     TEGAISRREQSRAEHYNPGDRIRAVLVNVERQGKGPQLQLSRASELLVRKLFEMEVPE
                     IYDGTVSIVNVSRDAGERSKVAVRSKDRDVDPVGACVGMKGSRVQAVIRELRGEKIDI
                     VQYDDDPSQYVRNSLNPATINRVGIRDYESREMEVIVAEDQLSLAIGKRGQNVRLASK
                     LVGWSLDIKSEAEKKAEVEAEMERMETASRELGSLPGIDKAISDLLLDAGFRTVEEVS
                     LASMDDLLTIDGIDEDDAIAIHDAAEQALEAQQARAAEEAAASEARSVDEADVTADEP
                     GDEQADDRTVVDASADQRTDGPAPSDVDFVEQTDERRSES"
BASE COUNT          397 a         1076 c         1037 g          610 t
ORIGIN      
        1 aacacggtgg ccagcacgcc gcgggcgcgg tcgagccggg cttcgagcac gacgccggtc
       61 gccggcttgt tcggattggc tttgagatcc ttcaggtccg cgaccagcag gatcatctcg
      121 agcagctcgt cgatgcccgt acgcttcttc gccgagacct cacacgagac gatgtctccg
      181 ccatactcct cgacgagcag gtttcggtcg gcgagctgct gcttgacgcg agcggggttc
      241 gccccgggct tgtccatctt gttgatcgcg acgacgatcg ggacatccgc cgcacgtgcg
      301 tggtcgatcg cctcgagcgt ctgcggcttg accccgtcgt cggcggcaac gaccagcacg
      361 acgatgtcgg tcgccttggc gccgcgcgcg cgcatcatgg tgaacgcctc gtgcccgggg
      421 gtatcgagga aggtgatctt gcggttccgg gcctcgacct ggtaggcgcc gatgtgttgc
      481 gtgatcccac cggcctccga gtcgacgacc gacgtttcgc gcaccgcgtc gagcagcgag
      541 gtcttgccgt ggtcgacgtg gcccatgacg gttaccaccg gcggtcgcgg tacgagctgg
      601 tcgggggtgt cgtcggcaac ctcggtctcg ccgagcaagt cgggtgtcgc gacctgctgc
      661 gcctcttctt cgaaggtgat gatgcgggcc tcggcaccga actctttggc aacctcgatc
      721 gcgacatcgg gttcgagcgg ctggttgatc gtcgccagaa tgccacgcgg gatcagtttg
      781 gcgatcacgt ccttcgactt gcggttcagc ttctcggcga gttccttgac ggtcacgcct
      841 tcggacagcg tgatctcggc cggcaccggc ggaagttcct tcggcacgcg cgccgtcggc
      901 acgactaccg cctggcgttc tttcttctcc ttcttgcgtc gcttcttccc gggcgtgacc
      961 ggggcggcgg ccggccggcg cacggtgccg ggcgggggcc cgtaaggtcg cggcgcggtc
     1021 ggaccggcgg cgacgcgggc cgcgggtggc ggcgacggct tgggtcgcaa ggtcgacgtg
     1081 gggggtacgc ggcgggcggg aaagacccgc ggcttgcgcg cggtcgtgcc ggcgatcgcg
     1141 ctcgtggcgg ccgcggccgc cgccggcgat tctggccggg ccggcgattt cctcgcggcg
     1201 ggcttgtcgg cgggcgccgc agcggcgggc accgcggtct cctcggccgc gggcgcgtgg
     1261 acggcgggct cggccggcgc cggggtctcc tccgcttggc ccgcgctcgg ctcgctcgcg
     1321 acgggcgccg cggcctcggc gggcggcatc ggctgctgcc gttcctggac ggccacccgc
     1381 tggcgctcgg cggcgatcgc cgacttgatc cgcatcgata ccaactcggc cgtcttctgg
     1441 tcgagcgtgc tgaggtcgga gcggacctct tggcccatac ggtcgagcaa ctccaggatc
     1501 agagcgcttt gaacgttcag gtccttggcg agttggtaaa ccctgacttt ggccatctat
     1561 ctccccgacg gggtcagcta cgattcgctt cttcgttcgt ccgtctgctc gacgaaatcg
     1621 acgtcggacg gtgcggggcc gtccgtgcgc tgatcggccg acgcgtcgac cacggtccga
     1681 tcgtcggcct gctcgtcgcc gggctcgtcc gccgtcacgt ccgcctcgtc caccgaccgg
     1741 gcctcggacg ctgctgcttc ctcggcggca cgggcctgtt gcgcttcgag cgcctgctcg
     1801 gcggcgtcgt ggatcgcgat cgcgtcgtcc tcgtcgattc cgtcgatcgt caacaggtcg
     1861 tccatcgagg ccaacgagac ttcctcgacc gtgcggaacc cggcgtcgag cagcagatcg
     1921 gagatcgcct tgtcgatacc gggcagactt ccgagctccc ggctggccgt ttccatccgc
     1981 tccatctcgg cctcgacctc ggctttcttc tcggcttcgc tcttgatgtc gaggctccaa
     2041 ccaacgagct tgctcgcgag ccgcacgttc tgaccgcgct tgccgatcgc gagcgacagt
     2101 tgatcttccg cgacgatcac ctccatctcg cgcgactcgt agtcgcggat cccgacacga
     2161 ttgatcgtcg ccgggttgag cgaattgcgc acgtactgcg acgggtcgtc gtcgtactgg
     2221 acaatgtcga tcttctcgcc gcgcaactcg cggatgaccg cttggacgcg cgagcccttc
     2281 atgccgacac atgcgcccac cgggtccacg tcgcggtctt tcgagcggac tgcgaccttg
     2341 ctgcgctcgc cggcatcgcg cgagacgttg acgatgctga ccgtcccgtc gtagatctcc
     2401 ggaacctcca tctcgaacag cttgcgcacc agcagctcgc tcgcgcgcga cagctggagc
     2461 tgcggtccct tgccctgacg ttcgacgttg accagcacgg cacgaatccg atcaccgggg
     2521 ttgtagtgct cggcccgcga ctgctcgcgt cgcgagatcg cgccttcggt gcgtcccaag
     2581 tcgaggatca tgtccccgcg atcgaagcgc ttgacctggc cggtcagcag ctcgccgacc
     2641 cggtcggcgt actcgtgcca gaccagctcg cgctcggctt ccttgacctt ctggtagatc
     2701 acctgcttgg ccgcctgggc cgcgatccgg gtcaacgggg cctcggcggt gtcgtacggg
     2761 gtctcgatca ccatccccaa ctcgacgtcc ggccgcgtca gcttcgcctc gtcgagcgag
     2821 atttcggtct gcgggtcgac gagctcgtcc tcctcgacga cgacctgctt cgagaagacg
     2881 gcgaagtcgc cggtggcgcg gtcgaatcgc gcaccgaagt ctttcttgct gcggtagtac
     2941 ttccgcgtcg cggcggcgta cgcttcctcg atggcctgaa tgacggtctc cgtgtcgaga
     3001 cccttctcgc ggccgatctg ctcaatcgtg tggtacagct cgctcgacat cgtcactgac
     3061 cctcatgcgc cgagacgttc gcaactatag cacggcggtt tctcgcttct tgacccgccg
//
LOCUS       DASPGN010000464         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1139873, whole genome shotgun sequence.
ACCESSION   DASPGN010000464 DASPGN010000000
VERSION     DASPGN010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139873"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VD788_13500"
     CDS             <1..439
                     /locus_tag="VD788_13500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77328.1"
                     /translation="ERDLRGGPRATIDGAREFDIALARGEWARAAEWFFATPHSATRR
                     TVGAAAKVRLGELLARNGHPHAALAAFQRALADHPVDDSRPAAHLGAAQVLLDDLANP
                     TGAYQHLYEVLDGGATPDQETTARRLLERLASSLRNLPRGPRR"
     gene            complement(446..1300)
                     /locus_tag="VD788_13505"
     CDS             complement(446..1300)
                     /locus_tag="VD788_13505"
                     /inference="COORDINATES: protein motif:HMM:NF013589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HXV77329.1"
                     /translation="MELPESRLYAFVDQSREFALHFLEGQRLIRDLAMTHSICGEGFA
                     YFRDVVLSVQPTIALLKQGEQFGFYVDSDAPFFRLKIEAGHHGVTRCALFPDAFREFP
                     PAMRGLVRVVKVFPRNRPPYQSVLEIDGLPLGEIVNRVLRDSYQVNAAIIVSQQSDQS
                     LLLLQLPPLAGAGDYDYSPDALAARRDGLLRPLERLFGRALSGKAGIVRGFASIGFEL
                     LAHKPIRFRCSCSHERMVGNLRLACGNQYGELFDPDQQALAITCEYCKSQYSVTRDDL
                     RRAAVRLN"
     gene            1437..2987
                     /locus_tag="VD788_13510"
     CDS             1437..2987
                     /locus_tag="VD788_13510"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HXV77330.1"
                     /translation="MDVVLLSRLQFALTIMFHYLFPPLTIGMGLVLVYLEGMYLKTSD
                     LRYHQAARFWTRIFALNFAIGVATGIVMEFEFGTNWAVYSRFVGDVFGSALAAEGIFA
                     FFLESGFLAVLVFGWDRVGPRMHFFSTLMVALGSIFSSVWIVVANSWQQTPAGHRYVQ
                     MSRDGQPWFIDGQPVMRAEIVDFWALVFNPSSVNRLVHVWIGSFILGSFFIMSISAWY
                     VLKRRHLEFARRSFTGALLLGTLSSLAMLASGHFQARSVAVEQPAKLAAFEGHFRTGK
                     ADLSLFGLPNGDEQRVDFDVAIPGGLSFLVHDDFDAPVIGLDRFRPEHRPPLTIPFVS
                     YHLMVALGFSFILLTVTASWMHWRGKLLESRPMMWLFVVAVIGAFAANQAGWVAAEVG
                     RQPWVVHPTIARDTAGEPLLDDDGFVRYRTQTVTMADGTTRERIGGLLTESGVSEVVR
                     AEQVLGSIVMFALLYLMLGSLWLFVLNRKIQTGPEEYDEAGRPGRSAGLLEVAGSKGP
                     LTGTPERS"
     gene            2994..>3576
                     /gene="cydB"
                     /locus_tag="VD788_13515"
     CDS             2994..>3576
                     /gene="cydB"
                     /locus_tag="VD788_13515"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HXV77331.1"
                     /translation="MDLHVVWFALLAVLLTGYAILDGFDLGVGILHPLARGDGERRLF
                     VNAIGPLWDGNEVWLVTFGGALFAIFPEAYATIFSGFYLPFMLLLFALITRAVSLEFR
                     GKLSSDRWRRLWDHGFFASSLTATLLFGVAVGNGMRGVPLNERGLYVGGLSDLVGPYQ
                     LLVGLLAVSLFAMHGAIYLFLKTEGAVQQRLGRW"
BASE COUNT          518 a         1168 c         1233 g          657 t
ORIGIN      
        1 cgagcgcgac ctgcggggcg ggccgcgggc gacgatcgac ggcgcgcggg agttcgacat
       61 cgcgctcgct cgcggggagt gggcgcgggc cgccgagtgg ttcttcgcca cgccgcactc
      121 ggccacgcgg cgcaccgtcg gcgcggcggc caaggtccgg ttgggcgagc tgctcgcccg
      181 gaacgggcat ccccacgcgg cgctcgccgc cttccagcgg gcgttggccg atcatccggt
      241 cgacgactcg cgcccggcgg cccacctcgg cgcggcgcag gtgctgctgg acgatctggc
      301 aaacccgacc ggggcctatc agcacctata cgaggtgctc gacggtggcg cgacgccaga
      361 ccaggaaacg accgcccgac gcttgctcga gcgactcgcc tcgtcgctgc gcaacctgcc
      421 gcgtgggccg agacgctagc cgcgttcagt tcagccggac ggcggcgcgc cgcagatcgt
      481 cgcgggtgac gctgtactgc gacttgcagt actcacaggt gatcgccagc gcctgctggt
      541 cggggtcgaa cagctcgccg tactggttgc cgcaggcgag acggaggttg ccgaccatcc
      601 gctcgtgcga gcacgagcaa cgaaagcgga tcggcttgtg cgccagcagt tcgaagccga
      661 tcgaggcgaa cccccgcacg atcccggcct tgccgctcaa cgcgcgcccg aacaagcgct
      721 cgagcgggcg caacaaaccg tcgcgccggg cggcgagcgc atccggcgag taatcgtagt
      781 cgccggcccc ggccagcggc gggagctgca acagcagcag gctctgatcg ctctgttgcg
      841 agacgatgat cgccgcgttg acctggtacg agtcgcgcag cacgcgattg acgatctcgc
      901 ccagcggtag tccgtcgatc tcgagcaccg actggtaggg cgggcggttg cgcgggaaga
      961 ccttgaccac gcgcaccaag ccgcgcatcg ccggcggaaa ctcgcggaac gcgtcgggga
     1021 acagggcgca gcgtgtgacg ccgtggtggc ccgcttcgat cttcaaccga aaaaacggcg
     1081 catccgagtc gacgtagaag ccgaattgct ctccctgctt gagcaacgcg atcgtcggct
     1141 ggacgctcag cacgacgtcg cgaaagtagg cgaagccctc gccgcagatc gaatgggtca
     1201 tcgccaggtc gcggatcagc cgttggccct cgaggaagtg cagggcgaat tcgcgcgatt
     1261 ggtcgacgaa agcgtaaagg cggctctccg gcagctccat ccgttcatta tgcgcgggtc
     1321 ggccggcgcc gcaaaaatgc ccagtccgat ttggcttgga cacctccggc gggctgggtt
     1381 agtttgtagc ctctcgcgac ggccgcagtc gcccacggcc gacgggtgac acgacgatgg
     1441 acgtggtcct gctctctcgc ctgcagttcg cgctgaccat catgttccac tacctgttcc
     1501 caccgctgac gatcggcatg ggtctggtgt tggtctacct cgaggggatg tacctcaaga
     1561 cgtcggatct gcggtaccac caggcggcga ggttctggac gcgcatcttc gcgctgaact
     1621 tcgcgatcgg cgtggcaacc ggcatcgtga tggagttcga gttcgggacg aactgggcgg
     1681 tctactcgcg cttcgtcggc gacgtattcg gctcggcgct ggccgccgag gggatcttcg
     1741 cgttctttct cgaatcgggg tttctcgcgg tgctggtctt cggctgggac cgggtcgggc
     1801 cgcgaatgca ctttttctcg acgctgatgg tcgcgctcgg atcgatcttc tcgtcggtct
     1861 ggatcgtcgt ggccaacagt tggcaacaga cgccggccgg tcaccgctac gtgcagatgt
     1921 cgcgcgacgg ccagccgtgg ttcatcgacg gccaaccggt gatgcgcgcc gagatcgtcg
     1981 atttctgggc gctggtgttc aacccgtcgt cggtgaaccg gctggttcac gtttggatcg
     2041 gcagcttcat cctcggctcg ttttttatca tgagcatcag cgcgtggtac gtgctgaagc
     2101 gccgccacct cgagttcgcc cgccgctcgt tcaccggcgc gttgctgctg ggcaccctgt
     2161 cgagcctcgc gatgctggcc agcggtcact tccaggcgcg ctcggtggcg gtcgagcagc
     2221 cggccaagct ggcggcattc gagggacact tccgcacggg caaggcggac ctcagcctgt
     2281 tcggcttgcc caacggggac gagcagcgcg tcgacttcga cgtcgcgatc cccggcggtc
     2341 tgagttttct cgtccacgac gacttcgacg ctccggtgat cggactcgac cgcttccggc
     2401 ccgaacaccg tccgccgctg acgatcccgt tcgtcagtta tcacctgatg gtggcgctcg
     2461 gcttctcgtt catcctgctg acggtgaccg cctcgtggat gcactggcgt ggcaagctgc
     2521 tcgaatcccg gccgatgatg tggctgttcg tcgttgccgt gatcggcgcc ttcgccgcga
     2581 accaagccgg ttgggtcgcg gccgaggtcg gccgtcagcc atgggtcgtt cacccgacga
     2641 tcgcgcgcga cactgccggc gagccgttgc tcgacgacga cggtttcgtc cgttaccgta
     2701 cccagacggt gacgatggcg gacggcacga cgcgcgagcg catcggcggt ctgctgaccg
     2761 agagcggagt cagcgaggtg gttcgcgccg agcaggtgct gggctcgatc gtgatgttcg
     2821 ccttgctgta tctgatgctc ggctcgctct ggctgttcgt gttgaaccgc aagatccaga
     2881 ccgggccgga ggaatacgac gaggccggcc ggccggggcg ctcggccggt ctgctcgagg
     2941 tggcgggctc gaagggcccg ttgacgggaa ccccggaacg gagctgacgg gcgatggacc
     3001 tgcatgtcgt gtggttcgcg ttgttggccg tgctgctgac cggctacgcg atcctcgacg
     3061 gcttcgatct cggcgtcggc attctccacc cgctggcccg cggcgacggc gagcgacggc
     3121 tgttcgtcaa cgcgatcggc ccgctgtggg acggcaacga ggtatggctc gtcaccttcg
     3181 gcggtgcact gttcgcgatc ttccccgagg catacgccac gatcttttcg ggcttctatc
     3241 tgccgttcat gctgctcttg ttcgcgctga tcacgcgcgc cgtcagcctc gagttccgcg
     3301 gcaagctgag ctcggatcgc tggcgccgcc tgtgggacca cggcttcttc gcgtcgagtc
     3361 tgaccgcgac gctgctgttc ggcgtcgccg tcggcaacgg gatgcgcggc gtcccgttga
     3421 acgagcgcgg attgtacgtc ggcggcctgt cggacctggt cgggccgtac cagctgctgg
     3481 tcgggctgct ggccgtctcg ttgttcgcga tgcacggcgc gatctacctg tttctcaaga
     3541 ccgagggcgc ggtgcaacag cggctcggtc gctgga
//
LOCUS       DASPGN010000465         2297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1609014, whole genome shotgun sequence.
ACCESSION   DASPGN010000465 DASPGN010000000
VERSION     DASPGN010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609014"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VD788_13520"
     CDS             complement(<1..871)
                     /locus_tag="VD788_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77332.1"
                     /translation="MTLVTAGQLEEGAARLEQASEDLSDPIVWCYSRMYLADAHFQLG
                     RAAADSTARERHYESARAWFRQASYCAGAELPADGESARPTPEQVEVIANFQTFGTED
                     LASQLERVSSAQSELLSSTQDHQAVADHWRLVTQMSGATREAEYRIALACFEDGDFDC
                     AATEAVAGSLLTDSTEPAEPARAIAERAIERAAEVGPPEADTYWRFVFRLVADWHRQE
                     PLDDGPDTVLENALGALDPAERLRLLELSEDCWVPLRVAEGLRDRQAEFVDEAKVAYA
                     CDPAGPAKNLLVDH"
     gene            complement(1108..>2297)
                     /locus_tag="VD788_13525"
     CDS             complement(1108..>2297)
                     /locus_tag="VD788_13525"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXV77333.1"
                     /translation="IPFGAGTSVEGHVAAVHGGISVDLTRMDRVLSISTDDLDCRVEA
                     GLTRNRLNEALRDRGLFFPVDPGADATIGGMVATGASGTTTVRYGAMRDNVLGLTVVL
                     ADGRTVRTGGRARKSSAGYDLTRLLIGSEGTLGIITEVGLRLYGVPESSSAAVCQYAD
                     VASAVRTVIAVVQLGIPVARCELLDDAMMAATIAYSGLAGFAVLPTLFLEFQGSAESV
                     RASAREVAEIAGEHGGTEFRWATAAGERRALWKARHDAYFACRAMAPGKAGITTDACV
                     PISALARCIEHTRRDVEASGLSAPLVGHVGDGNFHLIVLYDPGDAAERRRAERLNDRL
                     VRRALAAGGTCTGEHGIGLGKRGFLAREHGAGVEVMRAIKDALDPLGLMNPGKILPDR
                     PLR"
BASE COUNT          348 a          841 c          780 g          328 t
ORIGIN      
        1 agtggtcgac cagcaggttc ttggcggggc cggcgggatc gcacgcgtag gccaccttcg
       61 cctcgtcgac gaactcggcc tgacgatcgc gcagaccctc cgcgacacgg agcggaaccc
      121 agcaatcctc gctcaactcc agcaggcgca accgctcggc ggggtccaac gcccccagcg
      181 cgttttccag caccgtatcc ggtccgtcgt cgagcggctc ttgccggtgc caatcggcga
      241 cgagccggaa aacgaacctc caataggtat ccgcctccgg cgggccgacc tccgcggcgc
      301 gctcgatcgc ccgctcggcg atcgcgcggg ccggctccgc cggctcggtg gaatccgtca
      361 gcaggctgcc ggccacggcc tcggtggcgg cgcagtcgaa gtcgccgtcc tcgaaacagg
      421 cgagggcaat ccggtactcg gcctcgcgcg tcgcgcccga catctgggtg acgagacgcc
      481 agtgatcggc gacggcttgg tgatcctgcg tgctgctcag cagctcgctc tgtgcggacg
      541 agacgcgttc gagctgagag gccagatctt ccgtgccgaa ggtctggaag ttggcgatca
      601 cctcgacctg ctcgggcgtc ggccgagcgc tctcgccgtc ggcgggaagc tcggcgccgg
      661 cgcagtagga cgcctggcgg aaccacgctc gcgcgctctc gtagtgtcgc tcgcgcgccg
      721 tcgagtccgc cgccgcccgg cccagctgga agtgggcgtc ggccaggtac atccggctgt
      781 agcaccagac gatcgggtcg gacaggtcct ccgacgcctg ctcgagacgg gcggcccctt
      841 cctcgagctg acccgccgtg accagtgtca cgccggactc gtaagtctcc cagggagtcg
      901 ccgcagaagg ggcagaggcg gccgttccgt ccgccgcgga gggcgagacg gtttctttct
      961 ttttcgcggc ctcggccgtg gcaggcaaca acaacgccgc cgacaggacc gcgacgacgg
     1021 ctcggatctt gactcgcatg gctacggttc gattttacgg cgcggagaaa accgacgcag
     1081 cccgcggcgg acacagctgc tgagtattca ccgcaacggc cgatcgggca ggatcttccc
     1141 cgggttcatc agcccgagcg ggtcgagcgc gtccttgatc gcccgcatca cttcgacgcc
     1201 ggccccgtgc tcgcgcgcga ggaacccgcg cttgccgagc ccgatgccgt gctcgccggt
     1261 gcaagttcca cccgcggcga gcgcccgtcg caccagccgg tcgttgagcc gctccgcccg
     1321 ccggcgttcc gccgcgtcgc ccggatcgta cagcacgatc aggtggaagt tgccgtcgcc
     1381 gacgtgaccg acgagcggcg ccgacagccc ggaggcctcg acgtcgcggc gggtgtgctc
     1441 gatgcaccgg gcgagcgccg agatcggcac gcaggcgtcg gtcgtgatgc ccgccttgcc
     1501 cggagccatc gcccggcagg cgaagtaggc gtcgtggcgg gccttccaca acgcccggcg
     1561 ctcgcccgcg gcggtggccc agcgaaactc cgtgccgccg tgctcgccgg cgatctcggc
     1621 cacctctcgc gcactcgcgc gaaccgactc cgcgctgcct tggaactcga ggaacagcgt
     1681 cgggagcacg gcgaagcccg ccaggcccga ataggcgatc gtggccgcca tcatcgcgtc
     1741 gtcgagcagc tcgcaccgcg cgaccgggat gccgagctgg accacggcga tcaccgtgcg
     1801 caccgccgag gccacgtcgg cgtactggca gaccgcggcc gacgacgatt ccggtacgcc
     1861 gtacagccgg aggccgacct cggtgatgat gccgagcgtt ccctcggatc cgatcagaag
     1921 acgcgtcagg tcgtagccgg cggacgattt tcgcgcgcga ccaccggtcc gcaccgtgcg
     1981 tccgtcagcg aggacgacgg tcaagccgag cacgttgtcc cgcatcgccc cgtagcgtac
     2041 ggtcgttgtg cccgaggccc ccgtcgccac catcccgccg atcgtcgcgt cggcacccgg
     2101 gtcgaccggg aagaacaacc cccggtcgcg caacgcttcg ttcagccgat tgcgcgtgag
     2161 gccggcttcg acgcggcagt cgagatcgtc ggtcgagatc gacagcacac ggtccatccg
     2221 ggtcaggtcg accgagatcc cgccgtggac cgccgcgacg tgaccctcga ccgacgtgcc
     2281 cgcgccgaac gggatca
//
LOCUS       DASPGN010000466         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_671689, whole genome shotgun sequence.
ACCESSION   DASPGN010000466 DASPGN010000000
VERSION     DASPGN010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671689"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="VD788_13530"
     CDS             complement(<1..667)
                     /locus_tag="VD788_13530"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV77334.1"
                     /translation="MPSPFADRLRYGGNTSCLALPLDDDHHLIFDCGSGLREFGNSLA
                     ARPAGRARRFHVFFSHYHFDHVEGLPLFLPLYDSQSEFAFYGFRSGERSIREILEALI
                     RPPYFPVTLAGVPAKTRYVDIGDEPVAIGDIAVNSLPLNHPDGCLAFRLERGDRRIVY
                     ATDHEHGNPEADRALIDFARASSHLIYDATYTPTEYEESRRGWGHSTWYAAVQIAIAA
                     RVEN"
     gene            872..>2085
                     /locus_tag="VD788_13535"
     CDS             872..>2085
                     /locus_tag="VD788_13535"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXV77335.1"
                     /translation="MKNLVVVIGHALFVAAATAADEECGKLASVQNQVETKRNGGSDW
                     SSSTLGELLYASDRVRTAEASRAAILYSDQTLHRINEKSEVEVTPPDAGNPGVLRVIS
                     GQHYFSSRRPKDYGRIETPTVTAAIRGTEFVVDVDASGATTITMIEGVVDASNEHGGV
                     QVRAGEAAYAEPGKAPVRQLVVRPRDAVAWSLYYPRILGGADAARLDASGEPGRDLLA
                     AARSLSTGQVAVARSLIDQARTLDPNNPVALALASVVATAEDRRDDGRELARRAVEAD
                     PDSAAAALAMSLAHQADFDIAEARQMAERAARLNPESAEALARVAELRLAEGDTAGAL
                     EAAERAVRREPDSARALTVLGFVKLAELRSAEALELFDRAVAADPSFPLARLGRGIAM
                     SRRNLAAGREEM"
BASE COUNT          327 a          672 c          779 g          307 t
ORIGIN      
        1 ggttttcgac ccgagccgcg atcgcgatct gtacggccgc gtaccacgtg ctgtgacccc
       61 agccgcgccg cgactcttcg tactcggtcg gcgtgtaggt cgcgtcgtag atcaagtgcg
      121 acgacgcccg ggcgaagtcg atcaacgccc gatcggcctc tgggttcccg tgctcgtggt
      181 cggtcgcgta gacgatgcgc cggtcgccac gctcgagccg aaacgcgagg catccgtcgg
      241 ggtggttgag cgggagggag ttgacggcga tgtcgccgat cgcgaccggt tcgtcgccga
      301 tgtcgacgta acgcgtcttc gccgggacgc cggcgagcgt caccggaaag tacgggggtc
      361 ggatcaacgc ctcgaggatc tcgcggatcg agcgttcgcc ggaacggaag ccgtagaacg
      421 cgaactcgct ctgcgaatcg tagagcggga gaaacagggg tagaccctcg acgtggtcga
      481 aatggtagtg ggagaagaag acgtggaagc ggcgtgcgcg gccggccggt cgcgcggcga
      541 gcgaattgcc gaactcgcgc aggccggagc cgcaatcgaa gatcaggtga tgatcgtcgt
      601 cgagcgggag ggcgagacag ctcgtgttgc cgccgtagcg caggcggtcg gcgaagggcg
      661 acggcacggt tccccgagtg ccccaaaaac gcaatctgta ttcgttcgcg cccattcggt
      721 gccacccgtt cgtctcggcc gctggcgatt ctagcagccc ggcgcatcga ttccgtcgta
      781 ttccttggtc cgccgtcggc gatcgcggta gaatctcggc agccagaagg ggctggtcag
      841 cattccgggc agccggggga gtcgcccgca cttgaagaac ctcgttgtcg tgatcggcca
      901 tgcgctgttc gtcgcggccg cgaccgccgc ggacgaggag tgcggcaagc tggcgtcggt
      961 gcagaaccag gtcgagacca agcgaaacgg cggaagcgat tggtcgtcat cgacgctcgg
     1021 cgagctgctg tacgcgagcg accgcgtacg gaccgccgag gccagccggg cggcgattct
     1081 ctactccgac cagacgttgc accggatcaa cgagaagagc gaggtcgagg tgacgccgcc
     1141 cgatgccggc aaccccggcg tgctgcgagt gatctcgggc cagcattact tttccagtcg
     1201 ccgaccgaaa gactacgggc gcatcgagac gccgacggtg accgcggcga tccgcgggac
     1261 ggagttcgtc gtcgacgtcg acgcgagcgg agcgacgacg atcacgatga tcgagggtgt
     1321 ggtcgacgcc tcgaacgaac acggtggcgt gcaggtgcgc gccggcgaag cggcctacgc
     1381 cgagccgggc aaggcgccgg tccggcaact ggtcgtgcgc ccgcgcgacg ccgtcgcctg
     1441 gtcgctgtat tacccacgca tcctcggcgg tgcggatgca gctcggctcg acgcctccgg
     1501 cgagccgggt cgtgacttgc tggcggccgc tcggtcgttg tcgaccggac aggtggcggt
     1561 cgcgcgctcg ctgatcgacc aggcccgcac gctcgatccg aacaacccgg tcgcgctggc
     1621 gctggcatcg gtcgtcgcga cggcggagga tcggcgagac gacgggcgcg agctggcgcg
     1681 tcgcgcggtc gaggccgacc cggactcggc ggccgcggcg ctcgcgatgt cgctggccca
     1741 ccaggccgac ttcgacatcg ccgaggcacg ccagatggcg gagcgcgccg cccggttgaa
     1801 cccggagagc gccgaggcgc tggcgcgtgt cgccgagctg cgcctggccg agggagacac
     1861 ggccggagcg ctcgaggccg ccgagcgcgc ggttcgccgc gagccggact cggcgcgcgc
     1921 gctgacggtg ctcggcttcg tcaagctcgc cgagttgcgc tcggccgaag cgctcgagtt
     1981 gttcgaccgg gcggtcgcgg ccgacccgtc gtttccgctg gcccggctgg ggcgcggcat
     2041 cgcgatgtcg cggaggaacc tggccgccgg tcgcgaagag atgca
//
LOCUS       DASPGN010000467         4825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_87180, whole genome shotgun sequence.
ACCESSION   DASPGN010000467 DASPGN010000000
VERSION     DASPGN010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87180"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VD788_13540"
     CDS             <1..1354
                     /locus_tag="VD788_13540"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HXV77336.1"
                     /translation="VERYVANLLSSEDAPDVAAMNSGLLYVTDDDDGQFPATTESLIA
                     RFHPAAMQARRVYLNQFPQTAAGAAAARDEIAAALDEGSLLTVYFGHGGRTEWALENL
                     WRLADVGALGDTDRYSFVLALNCVNAFFTNTDAEPHSLGEWWTLVEGRGAVAGWAPSA
                     LGSLFNYQVLADRLYERIFVHKETTTGLAAWQALVASYVIDNVDIVNVRDMVFFGDPA
                     ARLPLDSDRDGLLERDEIAGGSDPDDADSDDDGLADAAEPAWDADGDGWINAADPDAD
                     DDGLPDGLEAGISVAGPGTDLAAGQFLADQDPASTTDPLDPDSDGGGAPDGAEDRNRN
                     GVVDPGETDPLDPSDDPLCSAVPAGEVTGLSLSKQPGGVRLSWDDPTPADPCRLYRVL
                     RATGDHAPADFGAFAPAVVATEPSWLAVGAAEDEPIVFYLIGARSPVSGDGPLGHYGF
                     "
     gene            complement(1395..3563)
                     /locus_tag="VD788_13545"
     CDS             complement(1395..3563)
                     /locus_tag="VD788_13545"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HXV77337.1"
                     /translation="MIPLVHWIERWRNLAPEEREARVRAVVDRLARRSPRIRALGLVA
                     EVPGWPRLARELSVPLDRMPDLLRADDPRRGPLFGTLASRVAGIVERRPDHVARILEQ
                     AGRVLDNRFSLLGHPVARRVDGSLDWHRDPVSGGDWPAAVYHLDVPIVRGDGSDVKYP
                     WELSRGQHLLALSQAWWFAPTVADGDAGGDLRRLCAARIVHDLYDWIRDNPRGLGVNW
                     TCTMEVAIRAFVWTAVLALMRGAPEFDDRLLRELVRSLWLHGRHIRAHLERNEGRPST
                     NHYLSDLVGLMAIAGALPELGESAVWHRLAHDALADEIDRQVHEDGVDFERSVPYHRL
                     ATELFLHAALLARAAGRPFGEPFERRLAAMLEFVACYTRPDGSAPQWGDNDDGRLLPL
                     DGWADPEPHDHRHLLALGGSWLARADLVARAEPRDVEARWLIDGVPPPADRPDDAAGR
                     SFDAARVYVMRALDLHLFVNCAPVGTAGLGNHTHNDVLSPCVWAAGTEWITDPGSGSY
                     TGQPALRNRLRATAAHATLQLGDREQNAIEPDTDGLFRVAERARPEVLAWRVSAERVE
                     LSGRHRGFGRPDEPWVHERTIVLDRARRHVWIVDRLERDGGESARAEAATLRLPLGPR
                     VEVVDAAAPPRPADEALLDAGEHEAEPPGEPRERRWIVLRDDRYRIARVGFDLPHGSR
                     IDVVSADHSPGYGTVVARRSIEVALAPHPSSFRARSVLVV"
     gene            complement(3567..>4825)
                     /locus_tag="VD788_13550"
     CDS             complement(3567..>4825)
                     /locus_tag="VD788_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXV77338.1"
                     /translation="EKDGHDYPYEWVRWTENRNMQEFLRLAATGRVALEPLIDRVLPV
                     ERAAEAYAALAGGPPAAPLAVLLDYTSAADRSPAADRRHTIDVVPSRSRGRGVAVAVV
                     GPGSFATAVHLPNLASLAPRARLRAVVGRAGVPAREAARTFAGAYATVDLDRVLGDDA
                     VDLVVIATRHDRHAALAERALRAGKAVFLEKPAALDREQLYALDAAVRQTGNPIVVGF
                     NRRFAPDVVALAGVLRRRRGPLVASYRIHAGALPAEHWLRGPEGGGRLIGEACHMLDL
                     LGFLVGAQRTAAAVHAPAPPAGRPDLGPRDNFVVTTAYADGSLATLTYTSLGDPAAAK
                     ERIEVSWDDKTAVIDDFVSLATSGVEIAARRSARPDKGHRAILERFVEHLEGDAPPPI
                     PWDEIADVTRLTFDLDDELRGSGRGE"
BASE COUNT          667 a         1724 c         1797 g          637 t
ORIGIN      
        1 tgtcgagagg tacgtagcga acctgttgtc gtcggaagat gcgcccgacg tcgccgcgat
       61 gaactccggt ttgttgtacg tcaccgacga cgacgacggt caattcccgg cgacgaccga
      121 aagcctgatc gcgcgctttc accccgcggc gatgcaggcc cggcgggtct acttgaatca
      181 gttcccgcag accgcggccg gcgccgcggc ggcgcgcgac gagatcgccg cggcgctcga
      241 cgagggctcg ctgttgaccg tctacttcgg tcacggcggg cggacggagt gggcactgga
      301 gaacctgtgg cggctcgccg acgtcggggc gctgggcgac acggaccgct acagcttcgt
      361 gctggcgctc aactgcgtca acgcgttctt caccaacacc gacgcggagc cgcattcgct
      421 gggagagtgg tggaccctgg tcgagggtcg cggggcggtg gccggctggg cgccgtcggc
      481 gctcggctcg ctgttcaact accaggtgct ggccgatcgg ctgtacgaga ggatcttcgt
      541 gcacaaggag acgacgaccg gcctggccgc ctggcaagcg ctcgtcgcgt cgtacgtgat
      601 cgacaacgtc gacatcgtca acgtgcgcga catggtgttc ttcggcgatc ccgccgcgcg
      661 gctgccgctc gactcggacc gcgacggcct cctcgagcgg gacgagatcg ccggcggtag
      721 tgatccggac gatgccgaca gcgacgacga cgggttggcc gatgcggccg agcccgcgtg
      781 ggacgcggac ggcgacggct ggatcaacgc ggccgacccc gacgccgacg acgacggctt
      841 gcccgacggt ctcgaggccg gaatcagcgt cgccggcccg gggaccgacc tcgccgccgg
      901 tcagttcctc gccgatcagg acccggccag cacgaccgac ccgctcgacc cggacagcga
      961 cggtggcggg gcgccggacg gcgcggagga tcgcaatcgc aacggcgtcg tcgatcccgg
     1021 cgaaaccgac ccgctcgacc cgtcggacga tccgctgtgc tcggccgtgc ctgccggcga
     1081 ggtgaccggc ctgtcgctct cgaagcagcc cggcggcgtc cggttgagct gggacgatcc
     1141 gactccggcg gacccgtgcc gcctctatcg cgtcctgcgc gcgacgggcg atcacgcgcc
     1201 ggccgacttc ggcgccttcg ctccggcggt cgtcgcgacc gagccgtcgt ggctcgccgt
     1261 cggcgcggcg gaggacgagc cgatcgtgtt ctatctgatc ggcgcccgct ctccggtctc
     1321 gggcgacggt ccgctcggcc actacggctt ctgagcggga cgtggcgccg cctcggggcg
     1381 ggaccttccc cggctcagac gacgagcacc gagcgcgcgc ggaacgacga cggatggggt
     1441 gcgagcgcga cctcgatcga gcggcgcgcg acgaccgtgc cgtagccggg tgagtggtcg
     1501 gccgagacga cgtcgatgcg cgagccgtgc ggcaggtcga aaccgactcg cgcgatccga
     1561 taccggtcgt cgcgcagcac gatccatcgc cgttcccgcg gctcgccggg cggctccgct
     1621 tcgtgctcgc cggcgtcgag cagcgcctcg tccgcgggtc gtggcggggc ggcggcgtcg
     1681 acgacctcga cccgcggccc gagcggcagg cgcagcgtgg ccgcctcggc tcgcgcggat
     1741 tcgccgccgt cgcgctcgag ccggtcgacg atccagacgt gacggcgtgc ccggtcgagg
     1801 acgatcgtcc gctcgtggac ccacggctcg tccgggcggc cgaatccccg gtgccgcccg
     1861 ctgagctcga cgcgctcggc cgacacccgc caggccagca cctcgggtcg cgcgcgctcg
     1921 gcgacgcgga acagaccgtc ggtgtccggt tcgatcgcgt tctgttcgcg gtccccgagc
     1981 tgcagcgtcg cgtgcgccgc cgtcgcgcgc agtcggttgc gtagcgccgg ctgcccggtg
     2041 tagctgccgc tgccgggatc ggtgatccac tcggtgccag cggcccacac gcacggcgac
     2101 agcacgtcgt tgtgcgtatg gttgccgagg ccggccgtgc cgaccggcgc acagttgacg
     2161 aacagatgca ggtcgagtgc ccgcatgacg tagacgcggg ccgcgtcgaa cgaccggccg
     2221 gcggcatcgt cgggccggtc ggcgggtggc ggcacgccgt cgatcagcca gcgcgcctcg
     2281 acgtcacgcg gctcggcccg ggcgaccagg tcggcgcggg cgagccagct tcccccgagc
     2341 gcgagcaagt gccgatggtc gtgcggctcc gggtcggccc agccgtcgag tggcaacagc
     2401 cggccgtcgt cgttgtcgcc ccactgcggc gcgcttccgt cgggccgggt gtagcaggcg
     2461 acgaactcga gcatcgcggc cagccgccgc tcgaacggct cgccgaacgg ccggccggcg
     2521 gcgcgcgcga ggagcgccgc gtgcaggaac agctcggtcg ccagacggtg gtagggtacc
     2581 gaccgttcga agtcgacacc gtcttcgtgc acctgccgat cgatctcgtc ggccagcgcg
     2641 tcgtgggcca gtcgatgcca gaccgcggac tcgcccagct ccggcagcgc gccggcgatc
     2701 gccatcagac cgaccagatc ggacaggtag tggttggtgc tcggtcgacc ctcgttccgt
     2761 tcgaggtggg cgcgtatgtg acgtccgtgg agccacagcg agcggaccag ctcgcgcagc
     2821 aatcggtcgt cgaactccgg cgccccgcgc atcagcgcga gcaccgcggt ccagacgaag
     2881 gcgcgaatcg ccacctccat cgtgcaggtc cagttcacgc cgaggccgcg cgggttgtcg
     2941 cggatccagt cgtacaggtc gtggacgatg cgcgcggcgc acagccgcct caggtcgccc
     3001 cccgcgtctc cgtcggcgac ggtcggtgcg aaccaccagg cctgcgacag tgccagcagg
     3061 tgctggccgc gggacagctc ccacgggtac ttgacgtccg acccgtctcc gcgaacgatc
     3121 ggcacgtcga ggtggtagac cgcggccggc cagtcgccgc ccgagaccgg gtcgcggtgc
     3181 cagtcgagcg acccgtccac ccgacgcgcg accggatggc ccagcagcga gaagcgattg
     3241 tcgaggacgc gccccgcctg ctcgaggatc cgggcgacgt gatccgggcg ccgctcgacg
     3301 atgccggcga cacgcgatgc gagcgtgccg aatagcggtc cacgccgcgg atcgtcggcg
     3361 cgcagcaggt cgggcatgcg gtcgagcggc acgctcagct cgcgcgcgag ccgcggccag
     3421 ccgggaacct ccgccaccaa gccgagggcg cggatgcgcg ggctgcggcg ggccagccga
     3481 tccacgacgg cgcgcacgcg cgcctcgcgc tcttccggcg cgaggttgcg ccaccgctcg
     3541 atccagtgaa cgagcgggat catcggctac tcgccgcgac ccgatccgcg cagctcgtcg
     3601 tcgaggtcga acgtcagccg ggtgacgtcg gcgatctcgt cccaaggaat cggtggcggg
     3661 gcgtccccct cgaggtgctc gacgaaccgc tcgaggatcg cgcggtgacc cttgtccggt
     3721 cgggccgagc ggcgggcggc gatctcgacg ccggacgtcg cgagcgagac gaaatcgtcg
     3781 atcacggcgg tcttgtcgtc ccacgagacc tcgatccgtt ccttggcggc tgccgggtcg
     3841 ccgagcgagg tgtaggtcaa cgtggcgagc gagccgtcgg catacgccgt cgtgacgacg
     3901 aagttgtcac gcggaccgag gtccgggcgc ccggccggcg gcgccggcgc gtgcaccgcg
     3961 gcggcggtgc gctgtgcgcc gacgaggaag ccgagcagat cgagcatgtg acaggcctcg
     4021 ccgatcaacc gccctccgcc ctccggcccg cgcagccagt gctccgcggg cagcgcaccg
     4081 gcgtggatcc ggtagctcgc cacgagcggg ccgcggcggc ggcggagcac gccggccagc
     4141 gcgaccacgt cgggcgcgaa tcgccggttg aagccgacga cgatcgggtt gccggtttgg
     4201 cgcacggccg cgtcgagcgc gtaaagctgc tcgcggtcga gcgccgccgg cttctcgaga
     4261 aagacggcct taccggcgcg caacgcgcgc tcggcgagcg ccgcgtgtcg atcgtgccgg
     4321 gtcgcgatca ccacgaggtc gacggcgtcg tctccgagca cgcggtcgag gtcgacggtg
     4381 gcgtaggccc cggcgaaggt gcgtgccgcc tcgcgcgccg ggactccggc acgaccgacg
     4441 acggcgcgca ggcgcgcccg tggagcgagc gaggcgagat tcggcaagtg gaccgcggtc
     4501 gcgaacgagc cggggccgac gacggcgacg gcgacgccgc gaccgcgcga gcgcgagggc
     4561 acgacgtcga tcgtgtgccg ccggtcggcc gccggcgatc ggtcggcggc agaggtgtag
     4621 tcgagcagca ccgcgagcgg agcggccggc gggccgccgg ccaacgccgc gtacgcctcg
     4681 gctgcgcgct cgacgggcag cacgcgatcg atcaacggct cgagcgcgac gcgccccgtc
     4741 gcggcgagtc gcaggaactc ctgcatgttg cggttctcgg tccaacgcac ccactcgtag
     4801 gggtagtcgt gcccgtcctt ctcgt
//
LOCUS       DASPGN010000468         5465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_322160, whole genome shotgun sequence.
ACCESSION   DASPGN010000468 DASPGN010000000
VERSION     DASPGN010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5465
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322160"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="VD788_13555"
     CDS             complement(<1..1251)
                     /locus_tag="VD788_13555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV77339.1"
                     /translation="MDTERSTALARYTGRYDLADGGDVVVDYVADGQIVLTDLDRDAR
                     SIDRFADFLERDEGDRFAWRAGEARPDATVTFRRDEHDRVTGLDWSAGGRSGRADRSD
                     GFAIREIRWRANDITITASLYQPVRPIGGVVMIHGSPPSSRDLVWYQTFAQPLADRGI
                     AVLLPDRRGSGLTSGDGFGATFDELAADTLSAVETLRSLGLADRGGVGLLGLSQGAWV
                     APLCASRDTRIAFVAHLSGSMTTPAEQVTHEIRQGLRRNGFTPEDQDRILALNDLLLA
                     AVAGRSAWREFAEARERLLRTDLAPAVEPFPGREDDPRTRFWRAGHDFDPVPLLARIR
                     VPLLFVYGKDDELDNVPVRRSVERLESWRATHGRADVSVRLFDGLGHSLCPSDDSGWV
                     APEVLRTITDWVTETLRAPPNGARP"
     gene            complement(1350..2561)
                     /locus_tag="VD788_13560"
     CDS             complement(1350..2561)
                     /locus_tag="VD788_13560"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HXV77340.1"
                     /translation="MSASGPPAPISRTRVASIDLIRGAVMILMAIDHVRVFSGIPAGG
                     PTAGVFLTRWITHFCAPAFVFLAGTSAYLYGRRHADLPRFLVTRGVWLIAIELTVLRV
                     AWTFNFDFRGYEMAGVIWVIGWSMIFLGALTKLPLAVTAAIGTLVIAGHNAFDGVLAS
                     VVPTLGDDLRGALWKIAYAGFFAGPIRVGPDGPVLHVLYTLIPWVGVIAAGYAFGKPL
                     TLEPARRDRFCLVLGAGATALFLLLRGLDLYGDPRPWSAVAGPTGAKAMPALLSFLNT
                     TKYPASLDFLLMTLGPTIAAIPLLERARGRLAGAVAVFGRVPFFFYVLHIPLIHALAI
                     VVSLLRLGEVSPWLFADHPMGNPRPPEGYAWGLPLLYGVWLVAIVLLYAACRWFARVK
                     ATRSSPWLRYL"
     gene            complement(2613..2948)
                     /locus_tag="VD788_13565"
     CDS             complement(2613..2948)
                     /locus_tag="VD788_13565"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV77341.1"
                     /translation="MIVIDVAGRAKFDPDRMGKATLALGDRLFAGLNAFEAGQRQRPH
                     VHRGQDKLCYVLAGRAEVRVGDEHGLVGPGDLVLFPAGVEHSLHNPGPERLLAMIILA
                     PPREGDPAD"
     gene            complement(3034..4140)
                     /locus_tag="VD788_13570"
     CDS             complement(3034..4140)
                     /locus_tag="VD788_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77342.1"
                     /translation="MNAKRLVFLASSLVLVLAAALTLRLVNAGPWFESRAAEPVTPDA
                     PRWSGDPPRAGVVPEPRTASAADLGFPRWADPGSNTAIRFQSDLDLIAPLGDGPENAA
                     LWFADFAVPAGARAADYADARRRAEDAGVRGGLLPDDPLVREAEAWVDQATMRFYPDS
                     WPADGWNTQIPNLLMMLDLAKTWSARANTAGDLETALADYRRAIRLGRLLRQEDAVII
                     SDLVGLAAIRIGLQGMYDRLVRDDEPRRAMVVALALGECAPQRLKTAETITRVDPSRY
                     VSTGWLGADVDVSDDLFRTLSAMAEKDPDRRFRLEAILALNLVRFEGTRRQRRETAAL
                     LERLAATDDYWVAELAGWAASTPPDLASRSDMHR"
     gene            complement(4137..4511)
                     /locus_tag="VD788_13575"
     CDS             complement(4137..4511)
                     /locus_tag="VD788_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77343.1"
                     /translation="MTDDHEPFGALRPATPPAALRDRALAAARAALATRPASPDVWTR
                     VARNRAVRFGWAAVVAALALAHVVVSLPKPATRARAAVVSPRLGSGSAPELAPLVRMA
                     AIDPAARGAGSGRSPADKENGS"
     gene            complement(4508..5083)
                     /locus_tag="VD788_13580"
     CDS             complement(4508..5083)
                     /locus_tag="VD788_13580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV77344.1"
                     /translation="MAAVKYRRRIDAAGDDPYDAEAWRGLFRAIAGGRVEALERLYDL
                     AAGRVYGLAAWRLGSAEDAADVTHDVFVRVAESADRLREVDDPRAWLLAVTHRLAVDH
                     ARRRRRHRTTPLEDHVFFEVDVSHRVERIAVTAVLARLPDKQREAIFLHHFAGCTFAE
                     VGRITGVPTFTAASRYRLGLNKLRAILEGES"
     gene            complement(5211..>5465)
                     /locus_tag="VD788_13585"
     CDS             complement(5211..>5465)
                     /locus_tag="VD788_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77345.1"
                     /translation="ALSLCGVGVAAGDDKWVVGTELDLVPFLSDDFVTDDEFEDNDLS
                     VIAGTWNEHPHARRGIHLVVQQALLPQPVGCRSHSDRRRS"
BASE COUNT          873 a         1897 c         1968 g          727 t
ORIGIN      
        1 tggacgggct ccgttcggcg gggctcgcag cgtctcggtg acccaatcgg tgatcgtccg
       61 gagcacctcg ggcgcgaccc agcccgaatc gtccgacgga cagaggctgt ggccgagccc
      121 gtcgaacaga cgcacggaga cgtcggcccg gccgtgcgtc gcccgccacg actcgagccg
      181 ctcgacgctg cggcgtaccg ggacgttgtc caactcgtcg tccttgccgt agacgaacag
      241 cagcggtaca cggatccgcg cgagcagcgg tacgggatcg aaatcgtgac ccgcccgcca
      301 gaaccgggtg cgcgggtcgt cctcgcgccc ggggaacggc tcgactgccg gcgcgaggtc
      361 ggtccgcagc aggcgttccc gggcctcggc gaactcgcgc cacgccgacc gtccggcgac
      421 cgcggcgagc agcaggtcgt taagggccag gatccggtcc tggtcttccg gcgtgaagcc
      481 gttccggcgc aagccctggc ggatctcgtg cgtgacctgc tcggcgggcg tcgtcatgct
      541 gccggacagg tgcgcgacga aggcgatccg cgtgtcgcga ctggcgcaca gcggcgcgac
      601 ccaggcgccc tggctcaggc cgagcaagcc gacccccccg cgatcggcga gaccgagcga
      661 tcgcaacgtc tcgaccgccg acagcgtgtc cgccgccagc tcgtcgaagg tcgcgccgaa
      721 cccgtcgccg ctggtcagcc ccgagccgcg ccggtcgggc aacagcaccg cgatccctcg
      781 atcggcgaga ggctgggcga aggtctggta ccagacgaga tcgcggctcg acggaggcga
      841 gccgtgaatc atcacgacgc cgccgatcgg cctcaccggc tggtagagcg aggccgtgat
      901 cgtgatgtcg ttcgcgcgcc agcggatctc acggatggcg aagccgtccg accggtcggc
      961 gcgccccgaa cgcccgccgg ccgaccagtc gagaccggtc acgcggtcgt gctcgtcgcg
     1021 ccggaacgtg acggtcgcat ccggccgtgc ctccccggcg cgccaggcga accggtctcc
     1081 ctcgtcccgc tcgaggaagt cggcgaaccg gtcgatcgac cgggcgtcgc gatcgaggtc
     1141 ggtgaggacg atctggccgt ccgcgacgta atcgacgacg acgtcgccgc cgtcggcgag
     1201 atcgtaccgg ccggtgtagc gagcgagagc ggtcgagcgt tcggtgtcca tcggccgctc
     1261 ctgcggcggt gtggcgagca cactcgttcc tgttcacgcc tcctatacgg ctcgctcgcg
     1321 atccggttgc ggccgacccc gcggggcgct cagaggtagc gcagccacgg gctgctccgc
     1381 gtcgccttga cccgggcgaa ccagcggcag gcggcgtaga gcagaacgat cgcgacgagc
     1441 cagaccccgt agagcagcgg cagtccccag gcgtaaccct cggggggccg cgggttcccc
     1501 atcgggtggt cggcgaacag ccacgggctc acctcgccca ggcgcaagag tgagacgacg
     1561 atcgcgaggg cgtggatcaa cgggatgtgg agcacgtaga agaagaacgg cacccgaccg
     1621 aagacggcga ccgcaccggc cagccgaccg cgcgcccgtt cgagcagcgg gatcgccgcg
     1681 atcgtcggcc cgagggtcat caacaggaag tcgagcgacg ccgggtattt cgtcgtattg
     1741 aggaaggaca gcagcgcagg catcgccttc gcgccggtcg ggcccgcaac ggccgaccac
     1801 ggacgagggt ctccgtagag gtcgaggccg cgcaacagga ggaacagcgc cgtcgcaccg
     1861 gctcccagga cgagacagaa acggtcgcgc cgcgccggct cgagcgtcag tggcttgccg
     1921 aaggcgtagc ccgcggcgat cacgccgacc cacgggatca gcgtgtagag gacgtggagc
     1981 acgggtccgt cggggccgac gcggatcggg ccggcgaaga agccggcgta ggcgatcttc
     2041 cacaacgcgc cgcgaaggtc gtcgccgagc gtcggcacga ccgacgcgag cacgccgtcg
     2101 aaggcgttat gcccggcgat gacgagcgtg ccgatcgccg cggtcaccgc gagcggcagc
     2161 ttggtcagcg ccccgagaaa gatcatcgac cagccgatga cccagatcac gcccgccatc
     2221 tcgtagcccc ggaagtcgaa gttgaacgtc caggccacgc ggagcacggt cagctcgatc
     2281 gcgatcagcc acactccgcg cgtgacgaga aagcggggaa ggtcggcgtg ccggcggccg
     2341 tagaggtatg cgctcgtgcc ggcgaggaag acgaacgcgg gggcgcagaa gtgggtgatc
     2401 cagcgcgtca ggaagacccc tgctgtcggc ccgccggcgg ggatgccgga gaagacgcgg
     2461 acgtggtcga tcgccatcag gatcatcacg gcgccgcgga tcagatcgat cgacgcgacg
     2521 cgggttcgtg aaatcggggc gggcgggccg ctggcgctca tgccgcgagc gagtttacgc
     2581 cacccgtcgg ggcgggggct ttcaacatct tgctaatctg ccgggtctcc ctcgcgcggg
     2641 ggcgcgagga tgatcatcgc cagcaggcgc tccgggccgg gattgtgcag cgaatgctcg
     2701 acgccggcgg ggaacagcac gagatcgccc ggaccgacga gaccgtgctc gtcaccgacg
     2761 cggacctcgg cgcgcccggc gagcacgtag cacagtttgt cctggccccg gtgaacatgg
     2821 ggccgttgcc gctgtccggc ctcgaacgcg ttgagcccgg cgaacagccg gtcgccgagc
     2881 gcgagcgtgg cctttcccat ccgatccggg tcgaacttgg cccgaccggc gacgtcgatc
     2941 acgatcatcg gggcctccgt ggcgtcgatt ccccgcggag ccgcggagga tcgcgcgtgg
     3001 tttccacggg aggatagccc aaccacggcc ggatcatcgg tgcatgtcgg aacggctcgc
     3061 gaggtcgggt ggcgtgctcg ccgcccaccc ggcaagctca gcgacccagt aatcgtccgt
     3121 cgccgccagg cgttccagca gcgccgcggt ctcgcggcgc tgtcggcgcg ttccctcgaa
     3181 acgcacgagg ttgagcgcca ggatcgcctc gaggcggaag cgccggtcgg ggtccttttc
     3241 cgccatcgcg gacagcgtcc ggaagaggtc gtccgatacg tcgacgtcgg cgccgagcca
     3301 gccggtcgag acgtagcgcg aagggtcgac gcgcgtgatc gtctcggccg tcttcagccg
     3361 ctggggtgcg cattcgccga gcgcgagcgc gacgaccatc gcacgccgcg gttcgtcgtc
     3421 ccggaccagc cgatcgtaca tgccttgcag accgatgcgg atcgcggcga gcccgacgag
     3481 gtcgctgatg atgacggcgt cttcttgtcg cagcagccgg ccgaggcgga tcgcgcggcg
     3541 gtagtcggcg agcgcggtct cgagatcgcc ggccgtgttc gccctggccg accacgtctt
     3601 cgccaggtcg agcatcatca gcaagtttgg aatctgggtg ttccagccat cggccggcca
     3661 cgagtccgga tagaaccgca tcgtcgcctg gtcgacccag gcctcggcct cacgcaccaa
     3721 cggatcgtcg gggagcaatc cgccgcgaac cccggcatcc tccgcccggc gtcgggcgtc
     3781 ggcgtagtcg gcggcccgtg cgcccgccgg aacggcaaag tcggcgaacc agagcgccgc
     3841 gttctccggc ccgtcgccga gcggggcgat caggtcgagg tcgctctgga accggatcgc
     3901 ggtgttcgag cccgggtccg cccagcgcgg aaagccgagg tcagcggccg acgccgtccg
     3961 cggctccggc acgactcccg cccggggcgg gtcgccgctc cagcgcggcg catcgggcgt
     4021 caccggctcg gctgcccggc tctcgaacca cgggccggcg ttgaccagtc gcagcgtcag
     4081 ggccgcggcg aggaccagca ccagcgagga cgcgaggaag accagacgtt tcgcattcat
     4141 gatccgttct ccttatccgc cggcgatcgg ccgctaccgg cgccgcgcgc ggccggatcg
     4201 atcgcggcca tgcgcacgag gggtgcgagc tccggtgcgg agcccgaccc caggcgcggc
     4261 gagacgaccg cggcacgcgc gcgcgtcgcg ggcttcggca gcgatacgac gacgtgcgcg
     4321 agggcgaggg ccgcgacgac cgcggcccag ccgaagcgca cggctcgatt gcgcgcgacg
     4381 cgcgtccaga cgtccggcga cgccggtcgc gtggcgagcg ccgcgcgggc cgcggcgagc
     4441 gcgcgatcgc gtagtgccgc cggcggtgtg gcgggccgca gcgcgccgaa cggctcgtgg
     4501 tcgtcggtca tgattccccc tccaggatcg cgcgcagctt gttcaggccg agccggtaac
     4561 ggctcgccgc ggtgaaggtc ggcacaccgg tgatgcgccc cacttcggcg aaggtgcagc
     4621 cggcgaaatg atgcaggaag atcgcttcgc gttgcttgtc ggggagtcgg gccagcacgg
     4681 ccgtcaccgc gatgcgctcc acacggtggg atacgtcgac ttcgaagaag acgtggtcct
     4741 cgagcggagt cgtgcgatgt cgtctgcgcc ggcgcgcgtg atcgacggcc aagcgatgcg
     4801 tgacggcgag gagccacgcc cgcgggtcgt cgacctcccg caggcggtcc gccgattcgg
     4861 cgacgcggac gaagacgtcg tgggtcacgt cggccgcgtc ttcggcggat cccaagcgcc
     4921 acgccgccaa accgtagacg cggcccgcgg ccagatcgta gaggcgctcg agcgcttcca
     4981 ccctcccgcc ggcgatcgcg cggaacaacc cgcgccaggc ctcggcgtcg tacgggtcgt
     5041 ctccggcggc atcgatgcgc cggcggtatt tgactgcggc catgtctacc aagacgacgc
     5101 ccggcggtcg ttttgtcaaa cgaaccgggg cggcctagaa catcaccccc agcttgaccg
     5161 atgcctcggg ggtcacgtcg atctcgaaga ccgccgacgg gaaggtcacc tcacgatcgc
     5221 cgccgatcgg aatgtgaacg gcagcccacg ggttgaggta gaagtgcttg ctgaaccacc
     5281 aagtgtatcc cccgccgagc gtgaggatgt tcgttccagg tcccggcgat gaccgacagg
     5341 tcgttgtcct cgaactcgtc gtcggtgacg aaatcgtcgg acaggaacgg caccaggtcg
     5401 agctcggtgc cgacgaccca cttgtcatcg cccgccgcga cacccactcc gcacagcgac
     5461 agcgc
//
LOCUS       DASPGN010000469         7949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1609508, whole genome shotgun sequence.
ACCESSION   DASPGN010000469 DASPGN010000000
VERSION     DASPGN010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7949
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609508"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VD788_13590"
     CDS             complement(<1..190)
                     /locus_tag="VD788_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXV77346.1"
                     /translation="MRSLRQLFDDSQVLDLGPLPAPSLEPSATVQQALQALVRGRRGA
                     VVVTRGVEPVGIFTERDAL"
     gene            362..772
                     /locus_tag="VD788_13595"
     CDS             362..772
                     /locus_tag="VD788_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77347.1"
                     /translation="MNVVRLAAVGFVVLGCVVGGSAAAETETVTLKGQFVWERSDKGI
                     PGELAAMFTPSGEGTWDVVFHFEWEGEMREWKGTATGNLGSGRLEGEAVEDRDRKRTF
                     AFRGAFTDGVFAGSHGQLRDGELSSGGTLTLAAP"
     gene            complement(797..1924)
                     /locus_tag="VD788_13600"
     CDS             complement(797..1924)
                     /locus_tag="VD788_13600"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV77348.1"
                     /translation="MNAQLPLITRFASSWLQRSWPSYCVIFVTSRCNARCVFCFNWEN
                     VFSKPKDPEITLAEFEALSRSMKPLLQLVLSGGEPFLRRDLGEIISAFYRNSGTRLVG
                     IPTNAYLPDAIEETTRQVLTECPELTLNLNLSIDGILGKHDAVRGVKGQFRKILDSYA
                     RLDALRRRFPNLTINAQSVISKYNVEDIEKTIRQLRDELELGFHAVGPVRGSTPEKDA
                     KEITSEQVRAIYTRTDKIRHEHNDGRLMSRIADSLLHRLREIELRAMDSKRREFVCLA
                     GRKMVVISHDAKVYPCEPLWLDDEQKRVFPQDGVMGDLRQTGFDIRPILASERAESVR
                     ADVDKKLCACMYGCAIYNSLLFAPSTYPATLKELILPSRQR"
     gene            complement(1921..2928)
                     /locus_tag="VD788_13605"
     CDS             complement(1921..2928)
                     /locus_tag="VD788_13605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV77349.1"
                     /translation="MARSSVIIPVRNDAERLTKLLASFEPRDWEQHEVLVVDDGSTDD
                     TVAAAARFPARVVRLERTVGPAAARNHGARVARWPILVFVDSDVVLGPGALTRLIGWF
                     DDSLASGVSTIASATPENPGFVARYCAVTDKHVCEHWDAPAGGAAGDRCTWFSTRLGA
                     IRADIFAEVGGFDARFERPSIEDAEFSVRLARSHTLVLDRGVAHTHHWPASPTVLLRR
                     VFTNAKLLMREMRDDGEVGRDVMPVTERAGRVLGGLGVLLLPATPLSAAAAIAAVGCL
                     LASAWCHRALFGAYYRAGGLRFTLGCSVLHYLTTCAGLVGACVGAVTPVAKTKLGQGQ
                     A"
     gene            3065..4825
                     /locus_tag="VD788_13610"
     CDS             3065..4825
                     /locus_tag="VD788_13610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV77350.1"
                     /translation="MESPRIPDRPPRDRWSERTAIAILVLAGWLTRLPALRAVALNPD
                     ESQYEATAAYLLASGASAFSMPHTSPATYTFYKLMARLFGPYPMFEVRIAVMLLVIAT
                     ALWVYRAVCRGDGPWSGLAAGLIVVVWSVYFEGLTANREWFCNALLVLGALLFLGHFG
                     RDDRRARLALFLSGVSCGAALGFKLHAGLLVLPLAIVCLWRSSYREQRPAALRALAWY
                     IAGGLSITLLGLVPFAWEGTLGSYLRSIWTDLFGYAAAEAPRGTAGGTVTAPFYRGLP
                     GRILLLAAYGFSALVMVGAWRRLVRRRAPAGPFPGEQVVDQWAVAYLAAALVSVSIGA
                     RFFGHYYLFLVPPVAVLVGRAIGWLSRPHGREAATMGAALAFLVLLVVDRAASWGGTA
                     DRSTWVYLALSVGLIAFGLSRPRRRLAPSVAAILAVEIALVGWGASGMLYPASAPGPG
                     KRFARLSAVLGERSAAGDRLYVWGWAPEIYSLTRLTPSSQFVVSMYLVDDTRPEPTDS
                     AIDRRYERMLMEELRSTPPRFIVDASRRSWTMVESGDPWLYDLDRYPGFELSGYLDRH
                     YRAVGRFDGCVLYERRPQSG"
     gene            complement(4813..6579)
                     /locus_tag="VD788_13615"
     CDS             complement(4813..6579)
                     /locus_tag="VD788_13615"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HXV77351.1"
                     /translation="MRRDRFGLLAGQLLTAGALCGLLLPAAARDRQATAVFARATSAP
                     LAQRDASIIEGSSKITGAILDKLTVDPDTPLPMIVTYDREVRDDDLDELAELGASVTS
                     VLDSLNGCSMWMTPRDAVQLEDQSYVESVALDAPMVAMLDVARPTAGHPVSSPRSWAV
                     AGRGVTVAVLDSGVADHPDLRERIRARVDIVGRIEQAAQDGGLLDTRLVSFDEPTWEL
                     AAAVEEAPSEPSDGGFDPNGHGTHVAGIIAGDGSASRGLYRGVAPEAAIVDVRVIDGN
                     GGGTTTGVIAGLEWVLRNKDRFGIRVVSMSLGHPVLEHPDHDPLVRMVERLWQSGVVV
                     VCSAGNWGYYGNFTINSPGNSPRVITVGSLTDWNSYDRTDDMISSYSSRGPTLGLNVM
                     KPDLVAPGNRILSLRSAGSRLDVEHPDSRLAFRRGPAYYFELSGTSMATPLVAGAVAL
                     MLEAEPGLNPDSIKARLMVSADKGVDDDPFARGAGSLDVARALGQRGLTDHAPSPRVF
                     RDEDEIGLMRVEDVGELWGDSALWSDTALWSDTALWSDTALWADTALWADTALWADTA
                     LWSDTALWSDTALWSDSLPQPD"
     gene            complement(6835..>7949)
                     /locus_tag="VD788_13620"
     CDS             complement(6835..>7949)
                     /locus_tag="VD788_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77352.1"
                     /translation="IPVSPPFNSIVGHPNQNKIGDYTQMVSDDTGAALAYAATFNDEQ
                     DVWFVRVGDCNDNGVHDSQDVQGGTSEDCDANGHPDECESLTETCDDGIDNDCSGATD
                     CADEACVGQPSCTCDFSGTCDGGEDCFSCPSDCATGALGCGNGVCEANAGEHCLNCPQ
                     DCAGLQSGNPNDQFCCGAVGGGNNPVPCTDARCNDQGFVCIKTFIPSCCGDLSCAQDE
                     DPCGCGVDCGAQTGEEARCGDGIDDDCDGASDCDDDECLSVAVTPPDGAPRVEAGRRA
                     AGHYLAWHVAGASSYDVVQGDLGVLRVGGDFATATTACLASGTTDVEIETAADGVDRW
                     YLVRPYFCPGPGSYDTGHSGQSASRDAGIAAAAVCP"
BASE COUNT         1141 a         2742 c         2785 g         1281 t
ORIGIN      
        1 agagcgcgtc gcgctcggtg aagatgccga cgggttcgac cccgcgcgtg acgacgaccg
       61 caccgcgccg cccgcgcacc agcgcttgca gtgcctgctg caccgtcgcg ctcggctcga
      121 ggctcggcgc cggaagcggg ccgaggtcga gcacctggct gtcgtcgaac aactggcgta
      181 acgacctcac gcgcccgatt ctagcgcgaa ccgcgaccga cgcgggcgtc gatcgcattc
      241 gacggattct cggggaaatc cgccgtcctc gccgcgtgcc cggcaccgtc gtccgccgtc
      301 tccgccgttc gcggaccgca cggcggcgtg gcatactccc tcctcctgag gaggaccgag
      361 gatgaacgtc gtccggctgg ctgctgtggg attcgtggtg ctggggtgtg tcgtcggcgg
      421 gagcgcggcc gccgagacgg agaccgtcac gctcaagggt cagttcgtct gggagcgctc
      481 ggacaagggg atccccggcg agctcgcggc gatgttcacg ccgtcgggcg aaggcacctg
      541 ggacgtcgtg ttccacttcg agtgggaagg cgagatgcgc gagtggaagg ggacggccac
      601 gggcaacctc ggctcgggca ggctggaggg cgaggcggtc gaagatcgcg accgcaagcg
      661 gacgttcgcc tttcgcggtg cgttcacgga cggcgtgttc gccggctcgc acggtcagct
      721 gcgcgacggg gagctgagct ccggtggcac gctgacgctg gccgcgccct gacggccggt
      781 cggccgtcga tccgggtcag cgttgtcggc tgggtaggat cagctccttg agcgtggccg
      841 gataggtcga cggggcgaac agcaggctgt tgtagatcgc gcagccgtac atgcacgcac
      901 acagcttctt gtcgacgtcg gcgcggacgc tctcggcgcg ctcggacgcg aggatcggcc
      961 ggatgtcgaa gccggtctgg cgcaggtcgc ccatcacccc gtcctggggg aagacccgct
     1021 tctgctcgtc atccagccac aacggctcgc acgggtagac cttggcgtcg tggctgatca
     1081 ccaccatctt gcgaccggcg agacagacga actcgcgtcg cttgctgtcc atcgcgcgca
     1141 gctcgatctc gcgcaggcga tggagcagtg aatcggcgat tcggctcatc agcctgccgt
     1201 cgttgtgctc gtgacggatc ttgtcggtcc gcgtgtagat cgcgcgcacc tgttcgctgg
     1261 tgatctcctt ggcgtccttt tccggagtgc tgccgcgcac cggcccgacg gcatggaacc
     1321 cgagctcgag ctcgtcgcgc agctgccgga tcgtcttttc gatgtcctcg acgttgtact
     1381 tcgagatcac gctctgcgcg ttgatcgtga ggttggggaa ccggcgacgc agcgcgtcga
     1441 gccgggcgta gctgtcgagt atcttgcgga actggccttt gacgcccctc accgcatcgt
     1501 gcttgcccag gatgccgtcg atcgacaggt tgaggttgag cgtcaactcc gggcattcgg
     1561 tcagcacctg ccgcgtcgtc tcctcgatcg cgtccggcag gtaggcgttg gtcgggatgc
     1621 cgaccagacg ggtaccactg ttgcgataga aggccgagat gatctcgccg aggtcgcgcc
     1681 tgaggaacgg ctcgccaccg gacagcacga gctgcagcaa cggcttcatg ctgcgggaca
     1741 gcgcctcgaa ttcggcgagc gtgatctcgg ggtccttggg cttgctgaag acgttctccc
     1801 agttgaagca gaagacgcag cgcgcgttgc agcgcgaagt gacgaagatc acgcagtacg
     1861 acggccagga tcgttggagc caggagctgg cgaaacgcgt gatcagcggc aattgggcat
     1921 tcatgcttga ccctgcccga gcttcgtttt cgcgacggga gtgaccgccc cgacacacgc
     1981 accgacgaga ccggcgcacg tcgtcaggta atgtagcacg gagcagccga gcgtgaatcg
     2041 caggccaccg gcgcggtagt aggcgccgaa cagagcgcgg tggcaccagg cggacgccag
     2101 cagacaaccg acggcggcga tcgccgccgc tgccgacaac ggggtcgccg gcaacagcag
     2161 gacgcccagc ccgcccagca cccgtccggc cctctccgtc accggcatca cgtcacggcc
     2221 gacctcgccg tcgtcgcgca tctcccgcat cagcaacttg gcgttggtga acacccgccg
     2281 caacagcacg gtcgggctgg ccggccaatg atgggtgtgg gccacgccgc ggtcgagcac
     2341 caacgtgtgg gaccgagcga gccggaccga gaactccgcg tcttcgatcg acgggcgctc
     2401 gaaacgcgcg tcgaaccctc cgacctcggc aaagatgtcg gcccggatcg cgccgagccg
     2461 cgtcgagaac caggtgcagc ggtcgccggc cgcgccgccc gccggtgcgt cccagtgctc
     2521 gcagacatgc ttgtcggtca ccgcgcagta gcgcgcgacg aagcccgggt tctccggtgt
     2581 cgcggaggcg atcgtcgaca cgccgcttgc gagcgagtcg tcgaaccatc cgatcagccg
     2641 ggtcagggcg ccgggcccca gcacgacatc ggagtcgacg aagaccagga tcggccagcg
     2701 cgcgacgcgc gcgccgtggt tgcgcgctgc ggcggggccg acggtccgct cgagccggac
     2761 gacgcgcgcg ggaaagcgcg cggcggcggc cacggtatcg tcggtgctgc cgtcgtcgac
     2821 gacgagcacc tcgtgctgct cccagtcgcg cggttcgaac gaggccagca gcttcgtcag
     2881 ccgctcggcg tcgttgcgca cgggaatgat gaccgacgat cgagccacag gccctcccgc
     2941 cgcggccggc gacggcggct tattactacg atgagtcgag gcgctttgca acgacgtggg
     3001 gtgccgcggc acgctccggt cgtcgcgtcg ccgtgctagt ctgaaccggc gaggaacgcg
     3061 acgcgtggaa tcaccccgaa tccccgatcg accgccgcgc gatcgttgga gcgaacggac
     3121 ggcgatcgcg atcctggtgc tggcggggtg gctgacgcga ctccccgcgc tgcgggccgt
     3181 cgcgctcaac ccggacgaaa gtcagtacga ggcgaccgcc gcctacctgc tggccagcgg
     3241 cgcctccgcc ttctcgatgc cgcatacctc gcccgcgacc tataccttct acaaactgat
     3301 ggcgcgcctc ttcgggccgt atccgatgtt cgaggtgcgc atcgcggtga tgctgctggt
     3361 catcgccacc gcactgtggg tctaccgtgc cgtttgtcgg ggggacggtc cgtggagcgg
     3421 cctcgccgcg ggcttgatcg tcgtcgtgtg gagcgtctac ttcgaagggc tgactgccaa
     3481 ccgcgagtgg ttctgcaacg cgctgttggt cttgggcgcg ctgctgtttc tcggccattt
     3541 cggccgcgac gaccggcgcg cgcggttggc gctgttcctg tccggcgtgt cgtgcggcgc
     3601 ggcgctgggg ttcaagctcc acgccggtct gctcgtcctg ccgctcgcga tcgtctgcct
     3661 gtggcgctcg tcgtaccgcg agcaacgccc ggccgcgctt cgtgcgctcg cgtggtacat
     3721 cgccggcggg ttgtcgatca cgctcctcgg gctcgtgccg ttcgcgtggg agggcacgct
     3781 cggctcgtac ctgcggtcga tctggaccga tctgttcggc tacgccgccg ccgaggcgcc
     3841 gcgcggaacg gccggcggga cggtgaccgc cccgttctac cgcggattgc cggggcggat
     3901 tctgttgctc gcggcatacg gattctccgc gctcgtgatg gtcggggcgt ggcgccgcct
     3961 ggtgcgacgc cgagcgccgg ccggtccgtt tccgggcgag caggtcgtcg atcagtgggc
     4021 ggtcgcgtac ctggcggcgg cgctggtcag cgtatcgatc ggagcgcggt tcttcggcca
     4081 ttactacctg ttcctcgtgc cgccggtggc cgtgctcgtc gggcgcgcga tcggctggtt
     4141 gtcgcggccg cacgggcgcg aggcggcgac gatgggagcg gcgctcgcat tcctcgtttt
     4201 gctcgtagtg gaccgcgcgg cgagctgggg cggcacggcg gaccgatcga cgtgggtcta
     4261 cctggcgctg tcggtcggcc tgatcgcctt cgggctgtcg cggccgcggc gacgcctggc
     4321 gccctcggtg gcggcgatcc tggcggtcga gatcgcgctc gtcggatggg gagcgagcgg
     4381 catgctctac cccgcctcgg cgccgggtcc gggtaagcgg ttcgcccggc tgagcgccgt
     4441 cctcggcgag cggagcgccg ccggcgaccg gttgtacgtc tggggctggg cgcccgagat
     4501 ctattcgctc acccggctca cgccgtcctc ccagttcgtc gtttcgatgt acctggtcga
     4561 cgacacgcga cccgagccga ccgactccgc gatcgaccgg cgctacgaga gaatgttgat
     4621 ggaagagctg cgcagcaccc cgcctcggtt catcgtcgac gcttcacgcc gcagttggac
     4681 gatggtcgaa tcgggcgatc cctggctgta cgacctcgat cgctaccccg gtttcgagct
     4741 gagcggctat ctcgatcgcc actaccgcgc ggtcggacgg ttcgacggct gcgtgctcta
     4801 cgagcggcga cctcagtccg gctgaggcag cgagtcggac cacagcgcgg tgtcggacca
     4861 caacgcggtg tcggaccaca acgccgtatc ggcccacaac gccgtatcgg cccacagcgc
     4921 cgtatcggcc catagcgcgg tatcggacca cagcgcggtg tcggaccaca gcgcggtgtc
     4981 ggaccacaac gccgagtcgc cccacagctc gccgacgtcc tcgacccgca tcaggccgat
     5041 ctcgtcctcg tcgcggaaga ccctgggtga cggcgcgtgg tcggtcagcc cgcgctgtcc
     5101 gagcgcccga gcgacgtcga gcgagcccgc gccgcgagcg aacggatcgt cgtcgactcc
     5161 tttgtccgcg gagaccatca gccgcgcctt gatcgagtcg ggattcagcc ccggctcggc
     5221 ctcgagcatc agcgccaccg cgccggcgac gagcggcgtc gccatgctcg tcccggaaag
     5281 ctcgaagtag taggccggac cccgccggaa cgccaagcgc gagtccgggt gctcgacgtc
     5341 gagccgcgag cccgccgagc gcaacgagag aatgcggttt cccggcgcga ccaggtcggg
     5401 tttcatcacg ttgaggccga gcgtcggccc gcgcgacgag tagctcgaga tcatgtcgtc
     5461 ggtgcggtcg tagctgttcc agtcggtcag cgagccgacg gtgatgacac ggggagagtt
     5521 gccggggctg ttgatggtga agttgccgta gtagccccag ttgccggccg agcagacgac
     5581 gacgacgccg gattgccaca gccgttccac catgcggacc aacggatcgt gatccgggtg
     5641 ctcgaggacc ggatggccga gcgacatgct gacgacccgg atgccgaatc gatccttgtt
     5701 ccgcagcacc cactcgagac cggcgatcac gccggtcgtc gtgccgccac cgttcccgtc
     5761 gatcacgcgg acgtcgacga tcgccgcctc gggcgcgacg ccgcggtaca gaccgcgcga
     5821 ggcgctgccg tcgccggcga tgatgccggc cacgtgagtg ccgtgaccgt tcgggtcgaa
     5881 tccaccgtcg gacggctcgc tcggcgcttc ctcgaccgcg gcggccagct cccacgtcgg
     5941 ttcgtcgaag ctcacgagac gcgtgtcgag cagcccgccg tcctgcgcgg cctgttcgat
     6001 ccgcccgacg atgtcgacac gcgcgcggat ccgctcccgc aggtccggat ggtcggcgac
     6061 gccggagtcg agcacggcga cggtgacgcc gcgaccggcc acggcccagc tccgcggcga
     6121 cgacaccggg tggccggccg tcggccgcgc gacgtcgagc atcgccacca tcggcgcgtc
     6181 gagcgcgacc gattcgacgt aggactggtc ctcgagctgc accgcgtcgc gcggggtcat
     6241 ccacatcgag cagccgttga gcgagtcgag caccgaggtg accgaagcgc ccagctcggc
     6301 cagctcgtcg agatcgtcgt cgcgcacctc gcgatcgtag gtcacgatca tcggcagcgg
     6361 cgtgtcgggg tcgacggtca acttgtcgag gatcgcaccg gtgatcttgc tggagccctc
     6421 gatgatcgac gcgtcgcgtt gggcgagcgg agcggaggtc gcccgcgcga agaccgccgt
     6481 ggcctgccgg tcgcgggccg cggcgggtag cagcagaccg cacagggcgc cggcggtcaa
     6541 caactggccg gcgagcaagc cgaatcggtc gcgtcgcatg gtggatctcc tctgttcgag
     6601 ggcacctgct cgcaagaccg atgcccgccg cccgggcggg tacggattcg tcgattcggc
     6661 ggacagaatc cgccacgtcg cccggcgccg ggcgggcgcg gtgtccgaca ctccgacacg
     6721 cgcgtgccgg acgccggcgg agtcgcgggg cgatcgtcct cgcggtcagc ggtttgacgt
     6781 cggtgtcccc cccggcgacg cgacgtgtcc ggttcccgaa cagctcgccg gtcgtcaggg
     6841 gcagacggcc gcggccgcga tgccggcgtc gcgcgacgcg ctctggccgc tgtgaccggt
     6901 atcgtagctg cccgggcccg gacagaagta cgggcgcacc aggtaccagc gatcgacccc
     6961 gtcggccgcc gtttcgatct cgacgtcggt cgtgcccgag gccaggcagg ccgtggtcgc
     7021 cgtcgcgaaa tcgccgccga cgcgcagcac gccgaggtcg ccctgcacca cgtcgtagct
     7081 cgatgcgccg gcgacgtgcc acgccaggta gtggcctgcg gcccgtcgcc cggcctcgac
     7141 ccgcggcgcg ccgtccggcg gcgtgacggc gaccgacaga cattcgtcgt cgtcgcagtc
     7201 gctcgctccg tcgcagtcgt cgtcgatgcc gtcgccgcag cgcgcctcct cgcccgtctg
     7261 cgccccgcag tcgacaccgc aaccgcacgg gtcttcgtcc tgggcgcagc tcaggtcccc
     7321 gcagcaggac gggatgaacg tcttgatgca gacgaacccc tgatcgttgc agcgagcgtc
     7381 ggtgcacgga accgggttgt tgcccccgcc gaccgcgccg cagcagaact gatcgttcgg
     7441 gttgccgctc tgcagaccgg cgcagtcctg tggacagttc aggcaatgct ccccggcgtt
     7501 cgcctcgcaa acgccgttgc cgcagcccag cgcgccggtc gcgcagtccg acgggcacga
     7561 gaagcagtcc tcacccccgt cgcacgtccc gctgaaatcg cacgtacacg acggttggcc
     7621 gacgcaggcc tcgtccgcgc aatcggtcgc gccgctgcaa tcgttgtcga tgccgtcgtc
     7681 gcaggtctcg gtcaggctct cgcactcgtc cgggtgcccg ttggcgtcac aatcctcgct
     7741 ggttccgcct tgcacgtctt gcgagtcgtg cacgccgttg tcgttacagt cgccgacacg
     7801 aacgaaccag acgtcctgtt cgtcgttgaa cgtcgcggca taggccagcg ccgctccggt
     7861 gtcgtccgac accatctgcg tgtaatcgcc gatcttgttc tgattcgggt gcccgacgat
     7921 cgaattgaac ggcggcgaca ccgggatcg
//
LOCUS       DASPGN010000470         8882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_672439, whole genome shotgun sequence.
ACCESSION   DASPGN010000470 DASPGN010000000
VERSION     DASPGN010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8882
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672439"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1925)
                     /locus_tag="VD788_13625"
     CDS             complement(<1..1925)
                     /locus_tag="VD788_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77353.1"
                     /translation="MYTRLVRPRRLGGVSIALLTLVAASPHAAATDPPDAAAGEVIEM
                     TALSWDFLEDLTVPLDKWRKGVEAEKNDDGVSFKSAGYSRTPRKVDDGTYVFSAHVNT
                     ANRNHQLTERMELTVKHAGGDRWELADQQVVDSHVGLYRATGMACAPFASFSFEREGI
                     ELTASNGSVCEAYFEGRVSSFVLRADDLAYDYAPPEHAALAPTGKDFYALHELARRTH
                     QSQLGIRPAAAFVRCDVTTCEELLEQRFTGLERKPADERASVGYDRTVADWIRPVVDD
                     VLKARKEDAFSGFRVLDEPGNRWFNLFLPTDINARDEGVQLAYDNWGVDDSGWEVKFY
                     VWPKRWDVPNQLGGAIYGYFSEETIRTTDPYELERRQGGLARWFEVESLRGTVDLATL
                     DVEQLQGDVEFGLRLAQETDTLPFGIISFDIGSGGEPKPITVNSIRVDGRELTWVKTG
                     PLTGIAILPEKMPAGSRVDVRMHWESQAILKFTSSYSYLPRQGWVPFVRFGDLIDEFE
                     LTIRSPVKYDVLGAGRRVEERVEGDVRVSTWRAAMPVNFPTVIFGRYRSDTPGKGVTA
                     KKLDGTEIPIEIHVDADSFAQEGIAPGALRPLAAQAANSINLYAAMTGVDYPFGGLNL
                     VNDPSGLYLYGQAPSSIL"
     gene            2037..2942
                     /locus_tag="VD788_13630"
     CDS             2037..2942
                     /locus_tag="VD788_13630"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV77354.1"
                     /translation="MRPEHAARVAILSAVSLSAACGGPGAPDAPAGPDPRLAAGRHEF
                     VTEDGAAMPYVVEGDGETALVLIHGWMCDRTFWDAQLPVLRERYLVITLDLPGHGEGG
                     ETRERWSVAGFGEDVAALVERLDLERVVLVGHSMGGEVALRAAALARGRVIGIVGVDT
                     LHDAEFDFARPDVAQMIAAVQQDFRGTCAAMVESMFVEESAESVEANVRRRACEESNA
                     AVGTALILDFVTIDLPAWFREAGVPIRAINAAAPNPTRVDTNRKYADFDAVLIDGVGH
                     FPHMTRPDEFNARLLQALDGLTTGR"
     gene            2985..4070
                     /locus_tag="VD788_13635"
     CDS             2985..4070
                     /locus_tag="VD788_13635"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXV77355.1"
                     /translation="MGERSRDGGRRPADRRDLLRLGLAGGVLALVGRTPVRADPPAGY
                     AASDVHRAHPLEFRLDSDYGPDLSDRAARLLVGATLDFLRAEHPDGLTLSFWELNPAE
                     MPYLPRHIESIVEHVFRGVKDQLALRPVDPLVVLALLYNESRFHPKVVSPSGAVGMAQ
                     FMPDTALEYGLAPIAHLDLWRRSRESRAAYLADRQRRIDAFLERHALERFDAERAIDG
                     AIASGGVELLGEYRALTEMPDPSQAALGEYVAAIEQTLAQHEFFWDGREPLERLDGRV
                     GYQAVTRAVRYIALRIAEHQGMSSTAVAAYNSGPEPVRVANPESILHRFGDIPPYPET
                     VRYVQRFMAVYSAIKYRLFRNAAGETA"
     gene            4177..4773
                     /locus_tag="VD788_13640"
     CDS             4177..4773
                     /locus_tag="VD788_13640"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV77356.1"
                     /translation="MERRHALPTVVLALLVTAPLAAHDLDEETDEIARLIELAPGIRL
                     ADVGAGDGRFGAEIAHRVGPSGHVYLTEIDDDELDELRALAERREAGNLTVLAGAEDD
                     TRLPDGCCDAVLLRYVVHHMGDPAAMHASVRRSLKTSGRLLVIEKTEPGDGIAEDDLI
                     DAVTRAGFRVVSRHPDWGGHDGHYAVLFRVEATGGGER"
     gene            complement(4803..5423)
                     /locus_tag="VD788_13645"
     CDS             complement(4803..5423)
                     /locus_tag="VD788_13645"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HXV77357.1"
                     /translation="MATAERPEPFEFSGGALCLDLANTLGDRPQGGSERLHDLADFVR
                     WCEQAGLIDGGEGDRLRAEAGRRPRAARTAWRRVIELRETIYGVFSAIAAGRGPRPGD
                     LGALNRSLTRALPHQRIEATGAGFVWTWAPPSSGFDHLSWPVVRSAAELLTSADAVRT
                     RECAADTCGWLFIDRSRARRRRWCDMKICGNRAKARRHYRRLKQLG"
     gene            5531..6535
                     /locus_tag="VD788_13650"
     CDS             5531..6535
                     /locus_tag="VD788_13650"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXV77358.1"
                     /translation="MQQAEPDLSGAWFREQLLARRRRLADVPRRREFEALIGEVDAAL
                     ERLDRGTFGACERCHDPIEARRLFGDPSTRVCLGCLTDAQQRALERDLESAAAAQRAL
                     LPRPLVSVAGWEIAHRYLPFGPVSGDYCDVLRPDPADGTTWFVLGDVAGKGVAASILM
                     GHLQALFRALVTVDPSPAELARRANRILCEGVLAGAFSTLLLGRMRPDGEVEICNAGH
                     EPARLVRRDGVTALGATGLPLGLFGESGYDTVRFRMAAGDCLLLYSDGMSEAVNGRGE
                     AYGAKRLDAFVGRVNGDPAGSVLDQWLDDLDTFCADAARQDDLTVMLVRRREEAGGMQ
                     "
     gene            6532..6945
                     /locus_tag="VD788_13655"
     CDS             6532..6945
                     /locus_tag="VD788_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77359.1"
                     /translation="MTDRYRVATLDDIPRGAEPGAGSYEWKPIRHHLGIRAFGVNAGI
                     ARQPGDWVVEDHTELDDNASGHEELYYVARGRARFTIDGNELDAPAGTLVFVADPATR
                     RSAKACEAGTTVLYVGARAGQAFTVSPWERKYFDA"
     gene            complement(6942..7373)
                     /locus_tag="VD788_13660"
     CDS             complement(6942..7373)
                     /locus_tag="VD788_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77360.1"
                     /translation="MRRLVPTLVVLFLLTALRADGPAIATPHVALSIEPSKTRVGLAR
                     VYLSIGELRWHDGMLEGRYTIRVPMSPSSNDSGTIRLHAPGSLEQIRRRGATLAGHAE
                     SAENGERSSVLCDVQRDGRLRIEITTDKRRLSFDTRYRTTG"
     gene            complement(7559..7634)
                     /locus_tag="VD788_13665"
     tRNA            complement(7559..7634)
                     /locus_tag="VD788_13665"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7599..7601),aa:Gly,seq:ccc)
     gene            8227..>8882
                     /locus_tag="VD788_13670"
     CDS             8227..>8882
                     /locus_tag="VD788_13670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV77361.1"
                     /translation="MSLEAGQSLSHYCLIEKIGEGGMGVVWKAIDTTLDREVAIKVLP
                     DTLAGDAERLARFEREAKLLAALNHPRIASLYGLHATDGGRFLSMELIEGKDLADVLT
                     SGRLPIERALRIALQIAEGLEVAHSRGVVHRDLKPGNVKLTADGDVKILDFGLAKALQ
                     GPARSPDVDLSRSPTVTVATQVGVILGTAAYMSPEQSRGHETDRRTDVWALGCVLYEM
                     L"
BASE COUNT         1344 a         3042 c         3113 g         1383 t
ORIGIN      
        1 aggatcgagc tcggcgcctg gccgtagagg tacagcccgc tcgggtcgtt gaccaggttc
       61 agcccgccga acgggtagtc gacgccggtc atcgcggcgt acaggttgat cgagttcgcc
      121 gcctgggccg ccagcgggcg cagcgcgccc ggcgcgatcc cctcctgggc gaagctgtcg
      181 gcgtcgacgt ggatctcgat cgggatctcg gtcccgtcga gcttcttcgc ggtcaccccc
      241 ttgccgggcg tgtccgagcg gtagcggccg aagatcaccg tcgggaagtt gaccggcatc
      301 gccgcgcgcc aggtcgacac ccgcacgtcg ccttcgaccc gctcttcgac gcggcgaccg
      361 gcgccgagca cgtcgtactt gaccggcgac cggatcgtca gctcgaactc gtcgatcagg
      421 tcgccgaagc gcacgaacgg cacccagccc tgccgcggaa gatacgagta ggacgaggtg
      481 aacttgagga tcgcctggct ctcccaatgc atccggacgt cgacgcgcga tcccgcgggc
      541 atcttctccg gcaggatcgc gatgccggtc aacggtccgg tcttgaccca ggtcagctcc
      601 cgcccgtcga cccggatcga attgacggtg atcggcttcg gctcgcctcc gctgccgatg
      661 tcgaagctga tgatgccgaa cgggagcgtg tcggtctcct gcgccagcct cagcccgaac
      721 tcgacgtcgc cctgcagttg ctcgacgtcg agcgtcgcca ggtcgaccgt gccgcgcagc
      781 gactcgacct cgaaccagcg agcgagcccg ccctggcggc gttcgagctc gtagggatcg
      841 gtggtgcgga tcgtctcctc gctgaagtaa ccgtagatcg cgccgcccaa ctggttcggg
      901 acgtcccacc gtttgggcca cacgtagaat ttgacctccc agccgctgtc gtcgacaccc
      961 caattgtcgt aggccagctg gacgccctcg tcgcgggcgt tgatgtcggt cgggaggaag
     1021 aggttgaacc aacgattgcc gggctcgtcg agcacgcgga agccggagaa cgcgtcttcc
     1081 ttgcgggcct tcagcacgtc gtcgacgacc gggcggatcc agtcggcgac ggtgcggtcg
     1141 tagccgaccg acgcgcgctc gtcggcaggc ttgcgctcga gcccggtgaa gcgttgctcg
     1201 agcagctcct cgcaggtcgt gacgtcgcaa cggacgaacg ccgcggccgg ccggattccg
     1261 agctggctct ggtgggtccg tcgtgccaac tcgtgcaggg cgtagaagtc cttgccggtc
     1321 ggcgccaggg ccgcgtgctc cggcggcgcg tagtcgtagg ccaggtcgtc cgcccgcagc
     1381 acgaatgacg agacccggcc ttcgaagtag gcttcgcaga ccgagccgtt gctcgcggtc
     1441 aactcaatcc cctcgcgctc gaaggagaac gaggcgaacg gcgcgcacgc catgccggtc
     1501 gcgcggtaca gcccgacgtg gctgtcgacg acctgttgat cggcgagctc ccagcggtcg
     1561 ccgcccgcgt gcttgaccgt caactccatc cgctcggtga gctggtggtt acggttggcg
     1621 gtgttgacgt gcgccgagaa aacgtaggtc ccgtcgtcga ccttgcgcgg tgtgcgggag
     1681 tagccggcgc tcttgaacga gacgccgtcg tcgttcttct ccgcctcgac gcccttgcgc
     1741 cacttgtcga gcggcacggt gagatcctcg agaaaatccc agctcagcgc cgtcatctcg
     1801 atcacctcgc ccgctgcggc gtcgggcggg tccgtcgccg cggcgtgcgg ggatgccgcg
     1861 accagggtca gcagcgcaat cgacacgccg ccgagacgac gcgggcgaac aagtcgggtg
     1921 tacatcgcga cctccgggat cgggctcacg gtctcgagga cgaggatacc aggattcgcc
     1981 ggtcgcggcc gcgaccggac gagcgtgcta ggctggcgcc cgaggagaac gcgacgatga
     2041 gacccgagca cgcggcgcgt gtggcgatcc tctcggccgt gtcgctgtcg gccgcgtgcg
     2101 gcggacccgg cgctcccgat gcgccggcag gaccggatcc tcggctggcc gcgggccgtc
     2161 acgaattcgt cacggaagac ggcgctgcaa tgccctacgt cgtcgagggc gacggcgaga
     2221 ccgcgctggt gctgatccac ggttggatgt gcgaccggac gttctgggac gcgcagctgc
     2281 cggttctgcg cgagcgttac ctcgtcatca cgctcgacct gccgggacac ggcgaaggcg
     2341 gggagacccg cgaacggtgg agcgtcgcgg gcttcggcga ggacgtcgcc gcgttggttg
     2401 aacggctcga tctagaacgg gtcgtgctcg tcggtcactc gatggggggc gaggtggcgt
     2461 tgagggccgc cgccctggcc cgcggcaggg tgatcggcat cgtcggcgtc gacacgctgc
     2521 acgacgccga gttcgacttc gcacgccccg acgtggcaca gatgatcgcc gccgtccaac
     2581 aggactttcg cggcacctgc gccgcaatgg tcgagtcgat gttcgtcgag gaatcggcgg
     2641 agtcggtcga ggccaacgtg cgccgcaggg cctgcgagga gtcgaatgcg gcggtcggga
     2701 cggcgctgat tctcgacttc gttacgatcg acctgccggc gtggttccgc gaggccggcg
     2761 tgccgatccg tgcgatcaac gccgcggcac cgaatccgac ccgcgtcgac accaaccgga
     2821 agtacgccga tttcgacgcg gtgctgatcg acggggtcgg gcacttcccg cacatgacgc
     2881 ggcctgacga gttcaacgcg cggctgttgc aggcgctcga cggattgacc accgggcgct
     2941 gaagcggagt tgcggatccg ggcgccagca cgcaccatcg tgagatgggt gaacgctcgc
     3001 gcgacggggg tcgccgcccc gcggatcgcc gcgacctgct gcgcctcggc ctggccggcg
     3061 gcgtgctggc gctcgtcggt cggacgccgg tccgcgccga cccgcccgcg ggctacgcgg
     3121 cgagcgacgt ccaccgggca cacccgctcg agttccggct ggacagcgac tacggcccgg
     3181 acctgtccga ccgcgccgcc cgattgctcg tcggggcgac gctcgatttc ctgcgcgcgg
     3241 agcatcccga cgggctgacg ctgagcttct gggaactcaa cccggccgag atgccgtatc
     3301 tgccgcggca catcgagtcg atcgtcgagc acgtctttcg cggggtcaag gaccagctgg
     3361 cgctgcgtcc ggtcgacccg ctcgtcgtgc tcgcgctgct ttacaacgaa tcgcgttttc
     3421 atccgaaggt cgtctcgccc tccggggcgg tcggcatggc gcagttcatg cccgacaccg
     3481 cactcgagta tggcctggcg ccgatcgcgc atctcgacct gtggcggcgc tcgcgcgagt
     3541 cgcgggcggc gtacctggcc gaccggcagc gacggatcga cgcctttctc gaacgacacg
     3601 cgctcgaacg cttcgatgcc gagcgcgcga tcgacggcgc gatcgcgagc ggcggcgtcg
     3661 agctgctcgg cgagtaccgc gcgttgaccg agatgcccga cccgagccaa gcggcgctcg
     3721 gcgagtacgt ggccgcgatc gagcagacgt tggcacagca cgagttcttc tgggacggtc
     3781 gcgagccgct tgaacggctc gacgggcggg tcggctacca ggcggtcacg cgggcggtgc
     3841 gctacatcgc gctgcgtatc gccgagcacc aggggatgag cagcaccgcc gtcgccgcct
     3901 acaactccgg acccgagccg gttcgcgtcg ccaacccgga gtcgatcctc caccgcttcg
     3961 gcgacatccc gccgtatccc gagaccgtgc gctacgtcca gcggttcatg gcggtctact
     4021 cggcgatcaa gtaccgcctg ttccgcaacg cggccgggga gacggcctga gcccgcccca
     4081 cgaccccacg cgtcgagcgc caccgtcggg tcgtcgtttc cgggtttaca acgttttacg
     4141 ccggcggcgg ccggacgggg cgatcatcgc gacgagatgg aacgccgcca cgccctgccg
     4201 accgtcgtgc tcgcgctgct cgtcaccgcg ccgctcgcgg cccacgacct cgacgaggaa
     4261 acggacgaga tcgcgcggct gatcgagctc gcgccgggga tccgcttggc cgatgtcgga
     4321 gcgggagacg gccggttcgg cgccgagatc gcccatcgtg tcggaccttc cggccacgtc
     4381 tacttgaccg agatcgacga tgacgagctc gacgagctac gagcgctcgc cgagcggcgc
     4441 gaggccggga acttgaccgt gctggccggc gcggaagacg acacccggct ccccgacgga
     4501 tgctgcgacg cggtgttgct gcgctacgtg gttcaccaca tgggcgatcc ggccgcgatg
     4561 cacgcgagcg tgcgtcggtc gctcaagacc agcggccggt tgctcgtgat cgagaagacc
     4621 gaaccgggcg acgggatcgc cgaagacgat ctgatcgacg cggtgacgcg cgccggcttc
     4681 cgcgtggtct cgcggcaccc cgactggggc ggtcacgacg gacactacgc cgtgctgttc
     4741 cgcgtcgagg cgacgggcgg cggcgagcgg tagcgcgagc gcgcggcggc cggcgccgac
     4801 ggtcagccga gctgcttgag gcgccggtag tgacgccgcg ccttggcccg gttgccgcag
     4861 atcttcatgt cgcaccagcg tcgtcggcgg gcccggctgc gatcgatgaa cagccagccg
     4921 caggtgtcgg ccgcgcactc gcgcgtccgc acggcgtcgg cggaggtcag gagctcggcg
     4981 gccgagcgga cgacgggcca cgacaggtgg tcgaaccccg aggacggcgg ggcccaggtc
     5041 cagacgaagc cggcgcctgt ggcctcgatc cgctggtgcg gcagggcccg ggtgagcgag
     5101 cggttgaggg cgccgaggtc acccggccgc gggccgcggc cggccgcgat cgctgaaaaa
     5161 acgccgtaga tcgtctcgcg cagctcgatc acccgacgcc aggcggtgcg cgcggcccgc
     5221 gggcgccggc cggcctcggc ccggaggcga tcgccctcgc cgccgtcgat cagaccggcc
     5281 tgctcgcacc agcgcacgaa atcggcgaga tcgtgcaggc gctcgctgcc accctgcggc
     5341 cggtcgccca gggtgttggc caggtcgaga cacaacgccc cgcccgaaaa ctcgaagggt
     5401 tccggtcttt ccgcggtggc catggcagta ccaatataac cgattcatgg ctcttgacag
     5461 gttatggaag acacggtatc ttgataacca gttagatcga tttatgtggt tataccacgg
     5521 gaggcgcgcg atgcaacaag ccgaacccga tctctccggt gcgtggtttc gcgagcagtt
     5581 gctcgctcgt cgcaggcgat tggccgacgt gccccgccgg cgcgagttcg aggcgctgat
     5641 cggcgaggtc gatgccgcgc tcgagcgact cgatcgcggc acgttcggcg catgcgaacg
     5701 ctgtcacgac ccgatcgagg ctcgccgctt gttcggcgat ccctcgaccc gcgtctgtct
     5761 cggctgtctg accgacgcgc agcagcgggc gctcgagcgc gatctcgagt cggccgccgc
     5821 cgcccagcgc gcgctgctgc cccgaccgtt ggtttcggtc gccggctggg agatcgctca
     5881 ccgctacctg ccgttcgggc cggtgagcgg cgactactgc gacgttctac gacccgatcc
     5941 cgccgatgga acgacgtggt tcgtgctggg cgacgtggcg ggcaaggggg tcgcggcgtc
     6001 gatcctgatg gggcatctcc aggcgctgtt tcgcgcgttg gtgaccgtcg atccctcgcc
     6061 ggccgaactg gcccggcgcg ccaaccggat cctgtgcgag ggcgtgctcg ccggcgcctt
     6121 ttcgacgctg cttctcggcc ggatgcggcc cgatggcgag gtcgagatct gcaacgccgg
     6181 tcacgagccc gcccggctcg tgcggcgcga cggcgtgacg gcgctcggcg cgaccggtct
     6241 gccgctcggc ctgttcggtg agagcggcta cgacacggtc cggttccgca tggcggcggg
     6301 tgactgtctg ctgctctaca gcgacgggat gtccgaggcg gtcaacggtc gtggggaggc
     6361 gtacggtgcg aagcgtctcg acgccttcgt cggccgggtc aacggcgatc cggccgggag
     6421 cgtgctcgat cagtggctcg acgatctcga cacgttttgc gcggacgcgg cgcggcagga
     6481 cgacctgacc gtgatgctcg tccgccgacg ggaggaagca gggggtatgc aatgacagac
     6541 cgataccgtg tcgccacgct cgacgacatc ccgcgggggg ccgagcccgg cgccggctcg
     6601 tacgagtgga agccgattcg ccatcacctc ggcatccgcg ccttcggcgt caacgccggc
     6661 atcgcgcgcc agccgggcga ctgggtcgtc gaggaccaca ccgagctcga cgacaacgcc
     6721 tccgggcacg aggagctgta ctacgtcgcg cgcgggcgcg cccgcttcac gatcgacggg
     6781 aacgagctcg acgcgccggc gggcacgctg gtgttcgtcg ccgacccggc gacgcgacgc
     6841 tcggcgaagg catgcgaggc cgggacgacc gtgctctacg tcggcgcccg cgccggtcag
     6901 gcgttcaccg tctcgccctg ggagcggaag tacttcgacg cctagccggt cgtacggtag
     6961 cgcgtgtcga acgacaggcg gcgcttgtcc gtcgtgatct cgatccgcag ccggccgtcg
     7021 cgctggacgt cgcacagcac gctgctgcgc tcgccgttct ccgcgctttc ggcatgaccc
     7081 gcgagcgtcg cgccgcggcg ccggatctgc tcgagcgagc cgggcgcgtg gaggcggatc
     7141 gtgccgctgt cgttgctcga cgggctcatc ggcacgcgga tcgtatagcg gccttcgagc
     7201 atgccgtcgt gccagcgcag ctcgccgatc gacaaataga cgcgggcgag gccgacgcgg
     7261 gtcttgctcg gctcgatgct gagcgcgacg tgcggggtgg cgatggccgg cccgtcggcg
     7321 cggagcgcgg tcagcaagaa aagtacgacc agcgtcggaa cgaggcgccg cacgatcgat
     7381 ctcccgggcg aacggggcag cgtacccccc aaaacatagg ctcccgcggc ggtggcgagc
     7441 aaccgggtgc cggccggcag agcgagaaac ccgcgacgca ggttagccga ttccgctccc
     7501 gattgcatct tcgaaatccg tcggcggccg gatgagcctg ccgcggcccc ggtcgggctg
     7561 gagcgggaaa cggggttcga acccgcgacc ctcagcttgg gaagctgatg ctctaccaac
     7621 tgagctattc ccgctcaacg acttacggct ggtgggacct cccagtgtcc aaacgggtgt
     7681 ccacccggta tgccctgaac tggctcagca tagcacgcgc gacgcatgga ccggaagcac
     7741 cctcgaaccg cgctcgatcg atctttggct gacagcgttg actcattcaa ctgcgactat
     7801 tcggattccg agttctcggg agagaagatt gaaacctcct acgttcgccg ttaccaccgc
     7861 ggctccgtgg cgacgcgcag tcagggcgat aagcgcgtcg ttctgaaact cgcgacgttg
     7921 gttgatctcc cacctctcgg catctccaat ctcacgtacc agcttccggc ttgccaccag
     7981 tcctggtcat tcggttccca ccgaatgcgc gcaagacgct gcagcgagtc gacaagccgc
     8041 cgtgccttcg gtgtccttgc tgcccgacga agctcggaga gaacgacaga cgaatgagga
     8101 tcgcccaggg cattcagact ccctacggag cactcacgct tcccggcttc agcgtcccgc
     8161 gggtgtacgc ctcgtccatc ggcccgaggt gtacactgcc gcgggaaggc accgtcggag
     8221 gccagtttgt ctctcgaggc cggccagtcc ctttcccact actgcctgat cgagaagatc
     8281 ggcgagggcg ggatgggtgt cgtgtggaag gcgatcgata cgacgctcga ccgcgaggtc
     8341 gcgatcaagg tgctccccga cacgctcgcc ggcgacgccg agcgcctcgc gcgcttcgag
     8401 cgcgaggcca agttgctcgc cgcgttgaac cacccgcgca tcgcatcgct gtacggcctg
     8461 cacgcgacgg acggcgggcg cttcctttcg atggagctga tcgagggcaa ggacctcgcc
     8521 gacgtcctga cctcgggccg gctcccgatc gagcgggcac tgcgcatcgc gctgcagatc
     8581 gccgaagggc tggaggtggc gcactctcgc ggcgtcgtgc accgcgacct caagccgggg
     8641 aacgtcaagc tgaccgccga cggcgacgtc aagatcctcg acttcggcct ggcgaaagcg
     8701 ctgcagggac ccgcacgaag cccggacgtc gacctctcgc gctcgccgac cgtgacggtc
     8761 gcgacgcagg tcggggtgat cctcggaacg gccgcctaca tgagccccga gcaatcgcgc
     8821 ggccacgaga cggaccgccg cacggacgtc tgggcactcg gctgcgtgct gtacgagatg
     8881 ct
//
LOCUS       DASPGN010000471         4979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1609949, whole genome shotgun sequence.
ACCESSION   DASPGN010000471 DASPGN010000000
VERSION     DASPGN010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1609949"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VD788_13675"
     CDS             <1..225
                     /locus_tag="VD788_13675"
                     /inference="COORDINATES: protein motif:HMM:NF036806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77362.1"
                     /translation="PAERAAERPVDPADLTADESHVLGIVGRAEPVHLDRLAELAAFG
                     VARLQSALFGLEIRGAVERQPGRYYVLRPK"
     gene            241..2811
                     /gene="topA"
                     /locus_tag="VD788_13680"
     CDS             241..2811
                     /gene="topA"
                     /locus_tag="VD788_13680"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HXV77363.1"
                     /translation="MAEALVIVESPAKAKTINKFLGRKYAVKASMGHVRDLPKRDLGV
                     DEDDFTPTYAVLPDKKKTLAELRRAAKDAKAVYLASDPDREGEAICWHLREELARDTD
                     AEFHRVLFNEITKRAILEAFEHPREIDLHKVEAQQARRILDRLVGYKISPLLWDKVRR
                     GLSAGRVQSVALRIIVEREREIQAFQSKEYWSLTALLEGDRPPQFPAKLVAKDGQKIE
                     LVSQSEVEAALADLGWRVSSVGAAPDGASAKIELGTIRDAATAVPFRVTSVEAREKRK
                     NPTPPFITSKLQQDAARQLGFSVSKTMRVAQGLYEGKELPGAGTIGLITYMRTDSTRV
                     SDEAIEAVRAYIGTTYGAESLPAKPRSYKVAKQSQEAHEAIRPTSLEYPPDEIKAQLG
                     RDEYRLYRLIWNRFVASQMRSAVYDVTRADIEAGPLTFRATGSVLKFPGFLAVYQESR
                     EEDVQARVDRIDDESEEREERTLPPLEQGQPLDPVALLPKQHFTQPPPRFSEATLVKE
                     LEENGIGRPSTYASILATIMDRDYVVKEKKRFQPTELGFLVNDLMVASFGDIVDVGYT
                     ARMEEELDRIEDGDLQWTTALKEFKDKFEADLARARVEMRDVKREAIPTDQTCEKCGK
                     PMVLKWGRFGRFLACSGYPDCKNTRDPGGDVAAGNGNEPPVLRSAAKTAKVKAEPSPI
                     EAEAPPCESCGKPMVLRRGRFGPFLACSGYPECKTTRKITKNADGQAEAKPERLLDEK
                     CPRCGAQLAVKQGRFGEFTSCSSYPDCRYVKMKETGVTCPECGKGQLVERRSKRGKVF
                     YGCANYPDCTFVLWRRPVDKACPDCDRGYLVERVTKKSGRELVCEAENCHHREMVTDG
                     "
     gene            2804..3127
                     /locus_tag="VD788_13685"
     CDS             2804..3127
                     /locus_tag="VD788_13685"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HXV77364.1"
                     /translation="MADALRPVDVTPFPNGEIGIRWSDGHESYYDAHALRCACACASC
                     VDEMTGHKVLDDDRVPRDVRARGFHPVGNYGVAIVWSDGHETGIYTFARLRELCGCGS
                     CAGRP"
     gene            complement(3138..3608)
                     /locus_tag="VD788_13690"
     CDS             complement(3138..3608)
                     /locus_tag="VD788_13690"
                     /inference="COORDINATES: protein motif:HMM:NF015629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HXV77365.1"
                     /translation="MKRKPIRIDWDDLESAVDDPNQEQNYYLDVVDGHVLLEGEGEEG
                     DYDDDGDDYASAPAAETAAGTRVYVDQLTTETKLEWIHAFIAEGEDLEPSFVEAIERA
                     LDSDDPAPAVIDALRESPDAKDRWYRYRSDRLHEWIERWLEDREIEAINPPPWR"
     gene            complement(3647..4816)
                     /gene="alr"
                     /locus_tag="VD788_13695"
     CDS             complement(3647..4816)
                     /gene="alr"
                     /locus_tag="VD788_13695"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXV77366.1"
                     /translation="MLNWVEVDGDAVLSNLREFQRRLGDGVRLGAVVKSNAYGHGMLE
                     VAALAREAGVRWLCVNNVDEAVELRSGGHHGPILVLGYVPYDRLSDVVEHGLRPVVAN
                     AETLARLETLASPESPLPVHLKLETGTHRQGISESDVVRIAGTIADSRVLQLEGISTH
                     FANIEDSTDHSFAEAQIAAFERVGATLARAGIDVPIRHAACSAATLLFTRTHLDLVRV
                     GISVYGLWPSKETYVSLRERGKPALALRPALTWKTRVGQVKLVEEGSYIGYGCTYRVT
                     RRSTIAVLPVGYHEGYDRGLSGVGYVLVHGRRAPIRGRVCMNMCMVDVTDIPGVALED
                     EVVLLGRQGEETIRAEQLADWCGTIAYEVVARINPSLARVVVRRESGQPLPQGRS"
BASE COUNT          842 a         1715 c         1716 g          706 t
ORIGIN      
        1 ccggccgagc gggccgccga gcggccggtc gatccggccg atttgacggc cgacgagagc
       61 catgtgctag gaatagtagg tcgcgcggaa cccgttcacc tcgaccgact ggccgagctg
      121 gcggccttcg gggtcgcccg cctgcagagc gccctgttcg gcctggagat tcggggcgcc
      181 gttgaacgac agccgggccg gtactatgtt ctgcgcccga aataaggagc cccggacggt
      241 atggcagaag cgctggtcat cgtcgagtcg ccggccaagg ccaagacgat caacaaattc
      301 ctcggccgca agtacgcggt caaagcgtcg atgggacacg tgcgcgacct gcccaagcgc
      361 gatctcgggg tcgacgaaga cgacttcacg ccgacctacg cggtcctgcc cgacaagaag
      421 aaaaccctgg ccgagctgcg acgtgcggcg aaggacgcca aggcggtcta cctggcctcc
      481 gacccggacc gcgagggcga ggcgatctgc tggcatctgc gcgaagagct ggcccgcgac
      541 accgacgccg agttccaccg ggtgctgttc aacgagatca ccaagcgggc gatcctcgag
      601 gcgttcgagc atccgcgcga gatcgacctg cacaaggtcg aggcacaaca ggcccgtcgg
      661 atcctcgatc ggctcgtcgg ctacaagatc agcccactgc tgtgggacaa agtgcggcgc
      721 gggctgtccg ccggccgcgt ccagagcgtg gcgctgcgca tcatcgtcga gcgcgagcgc
      781 gagatccagg ccttccaatc gaaggagtac tggtcgctga cggcgctgct cgagggcgat
      841 cgaccgccgc agtttccggc caagctggtg gcgaaggacg gccagaagat cgagctggtc
      901 tcgcagtcgg aggtcgaagc cgcgctcgcc gatctcggct ggcgggtgag ctcggtcggt
      961 gccgcccccg acggcgcctc ggccaagatc gagctcggga cgatccgaga cgccgccacc
     1021 gccgtaccgt tccgcgtgac gtcggtcgag gcgcgagaga agcgcaagaa ccccactccg
     1081 ccgttcatca cctcgaagct gcagcaagat gccgcgcggc agctcggctt cagcgtgtcg
     1141 aagacgatgc gcgtcgcgca ggggctgtac gaaggcaaag agctacccgg cgccggcacg
     1201 atcggtctga tcacctacat gcgcaccgac tcgacgcgcg tgtcggacga ggcgatcgag
     1261 gcggtgcgcg cctacatcgg cacgacctac ggcgccgaga gcctcccggc gaagccgcgc
     1321 agctacaagg tggccaagca gtcgcaagag gcgcacgaag cgatccgccc gacctcgctc
     1381 gagtacccgc ccgacgagat caaggcgcag ctggggcggg acgagtaccg cttgtaccgt
     1441 ctgatctgga accgcttcgt cgcgtcgcag atgagatcgg cggtctacga cgtgacgcgc
     1501 gccgacatcg aggccggccc gttgaccttc cgcgccaccg gctcggtgct gaagtttccc
     1561 ggcttcctcg ccgtctacca ggaatcgcgc gaggaagacg ttcaggcccg cgtcgatcgc
     1621 atcgacgacg agagtgagga gcgcgaggaa cgaacgctgc cgccgctcga gcagggccag
     1681 ccgctcgacc ccgtcgcgct gctgcccaag caacacttca cccagccgcc gccgcgcttc
     1741 tccgaggcga cactggtcaa ggagctggaa gagaacggca tcggccgccc gtcgacctac
     1801 gccagcatcc tcgcgacgat catggaccgc gactacgtcg tcaaggagaa gaagcggttc
     1861 cagccgaccg agctgggctt cctcgtcaac gacctgatgg tcgcatcgtt cggcgacatc
     1921 gtcgacgtcg gctacacggc gcgcatggaa gaagagctcg accggatcga ggacggcgac
     1981 ctgcagtgga cgaccgcgct gaaggagttc aaagacaagt tcgaggcgga cctggcccgc
     2041 gcccgggtcg agatgcgcga cgtcaagcgc gaggcgatcc cgaccgacca aacctgcgag
     2101 aagtgcggca agccgatggt gctcaagtgg gggcgcttcg gccggttcct cgcctgctcg
     2161 ggctaccccg actgcaagaa cacgcgcgac ccgggcggcg acgtcgcagc cggcaacggc
     2221 aacgagccgc cggtgctgcg ctcggcggcc aagacggcca aggtgaaggc cgagccgagc
     2281 ccgatcgagg ccgaagcgcc gccgtgcgag agctgcggca agccgatggt gctgcggcgc
     2341 ggccgcttcg gtccgttcct cgcctgctcg ggttaccccg agtgcaagac gacgcgcaag
     2401 atcacgaaga acgccgacgg gcaggccgag gccaagcccg agcggctgct cgacgagaag
     2461 tgtccgcgtt gcggcgccca gctcgcggtc aagcaaggtc ggttcggcga gttcacctcc
     2521 tgctcgtcct accccgactg ccgttacgtg aagatgaagg aaaccggggt gacctgcccc
     2581 gagtgcggca agggccagct cgtcgagcga cgcagcaagc ggggcaaggt gttctacggc
     2641 tgcgccaact accccgactg caccttcgtg ctctggcgcc ggccggtcga caaggcctgc
     2701 cccgactgcg accgcggcta cctcgtcgag cgggtgacga agaagagcgg ccgcgagctg
     2761 gtgtgcgagg ccgagaactg ccaccaccgc gagatggtca ccgatggctg acgcgctgcg
     2821 gccggtcgac gtcacgccct ttcccaacgg cgagatcggc atccgctgga gcgacggtca
     2881 cgagtcgtac tacgacgcgc acgcactgcg ctgtgcctgt gcctgcgcgt cgtgcgtcga
     2941 cgagatgacg gggcacaagg tgctcgacga cgaccgcgtg ccgcgcgacg tgcgggcacg
     3001 tgggttccac ccggtcggca actacggcgt ggcgatcgtc tggtccgacg gccacgagac
     3061 cgggatctac accttcgcgc ggctgcgcga gctgtgcggt tgcgggtcgt gcgccggccg
     3121 gccctgaccc gcgccggtca gcgccaggga ggcgggttga tcgcctcgat ctcgcggtcc
     3181 tcgagccaac gctcgatcca ctcgtgcagg cgatccgagc ggtagcggta ccagcgatcc
     3241 ttggcgtccg ggctttcgcg caaggcgtcg atgaccgccg gcgccgggtc gtcggagtcg
     3301 agcgcccgtt cgatcgcctc gacgaaggac ggctcgaggt cctcgccctc ggcgatgaag
     3361 gcgtggatcc actcgagctt cgtctcggtc gtcagttggt cgacgtagac ccgcgtcccg
     3421 gcagcggtct cggccgcggg cgcgctggcg tagtcgtcgc cgtcgtcgtc gtagtccccc
     3481 tcctcgccct cgccttccag cagcacgtgg ccgtcgacga cgtcgaggta gtagttctgc
     3541 tcctgattgg gatcgtcgac ggccgattcg agatcgtccc agtcgatccg gatgggcttg
     3601 cgcttcatgg ctgtcgttgg aatctacccc cggaggcgcg cgaaaatcaa gaccggcctt
     3661 ggggcaacgg ctgcccgctc tcgcggcgca cgacgacccg cgcgagcgac gggttgatgc
     3721 gggccaccac ctcgtaggcg atcgtgccgc accagtcggc cagctgctcg gcgcggatcg
     3781 tctcctcgcc ctgacgaccg agcagcacga cctcgtcctc gagcgcgacg ccggggatgt
     3841 cggtgacgtc gaccatacac atgttcatgc agacccggcc gcggatcggc gcgcgccggc
     3901 cgtggacgag cacgtagccg acccccgaca gcccgcggtc gtagccctcg tggtagccga
     3961 ccggcagcac ggcgatcgtc gagcggcgag tcacgcggta ggtgcagccg tagccgatgt
     4021 agctgccctc ctcgacgagc ttcacctgcc cgacccgcgt cttccacgtc agcgccggtc
     4081 gcagcgcgag cgcgggcttg ccgcgctcgc gcaacgagac gtaggtctcc ttcgacggcc
     4141 acaggccgta gaccgagata ccgacgcgta ccagatcgag gtgggtgcgg gtgaacagca
     4201 acgtcgccgc cgagcacgcc gcgtggcgga tcggcacgtc gataccggcg cgggccaagg
     4261 tcgccccgac gcgctcgaac gcggcgatct gcgcttcggc gaagctgtgg tcggtcgagt
     4321 cttcgatatt ggcgaagtgg gtgctgatcc cctcgagctg cagcacgcgc gagtcggcga
     4381 tcgtgcccgc gatgcgcacc acgtccgact cggagatgcc ctgccggtga gtgccggtct
     4441 cgagcttgag gtgcacgggg agcggcgatt cgggcgaggc caacgtctcg agccgggcca
     4501 gcgtctcggc gttggccacg accggccgca ggccgtgctc gacgacgtcg gacagccggt
     4561 cgtacgggac gtagcccagc accaggatcg gaccgtggtg tccgccggac cgcaactcga
     4621 ccgcctcgtc gacgttgttg acgcacagcc agcgcacacc ggcctcgcgc gccagcgcgg
     4681 ccacctcgag catgccgtgg ccgtaggcgt tggacttcac caccgccccg agccgcaccc
     4741 cgtcgccgag ccggcgctgg aactcacgca agttcgacaa gacggcgtcg ccgtcgacct
     4801 cgacccagtt cagcaacgtc ccgttctccg ggggcaaccg ggcgccccgc cggggtccat
     4861 cgtaggtccg aaaccgcccg ccgaccaccg cgatgcaacc aaatcggccg ctgccccgtt
     4921 tgttcggagg ggtggacggc cgcggacggc ccacgcggcg tgggaggagc catgagccg
//
LOCUS       DASPGN010000472         9504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_906968, whole genome shotgun sequence.
ACCESSION   DASPGN010000472 DASPGN010000000
VERSION     DASPGN010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906968"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VD788_13700"
     CDS             complement(<1..224)
                     /locus_tag="VD788_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77367.1"
                     /translation="MDNRIYLERRARLMREVSAGLIVVRGGGAEGVNPNLVYLTGIRE
                     PRAALVLARAGVRVGTGRRSPGPAYVLGRQA"
     gene            complement(217..1986)
                     /locus_tag="VD788_13705"
     CDS             complement(217..1986)
                     /locus_tag="VD788_13705"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXV77368.1"
                     /translation="MSNPTNHAVAGWSRRRMLALFGAAGTGAVFSRALLTLAADRATV
                     DEGMIRQAEWISGIELDNERRALMVEGMQELVADFEQLRQIPLDNGLPPALLFSPQAL
                     HPAPEGRNESRARPLSSLPGPRPDDDELAYLPVSRLSALIRTREISSEELTGIYLDRL
                     ERFDPRLELVITRTAELALEQARRADRELAAGAYRGPLQGIPWGAKDLLAVPPFPTTW
                     GATPYKDQVRSEQATVVSRLAGAGAVLTAKLTLGALAWGDVWFGGRTRNPWNVEQGSS
                     GSSAGPAAATAAGLVGFAIGSETWGSIVSPCSRCGVTGLRPSFGRVSRYGAMALSWSM
                     DKLGPIARSVEDCALVFDAIHGRDWLDPTVVDRPFDWPLERDPRSLRVGYVAKLFEQE
                     IAEDITDPEERELAEESLRFDRRTLARLEEIGIELIPIELPDSVPVAALSFILTAEAA
                     TAFDELTRGGRDRELVRQVANAWPNVLRQGQFIPAVEYLRANRIRWQLMQDMEQCLAG
                     VDVYVCPSFGGDNLLLTNLTGHPSVVLPNGHRASDGTPTSITFNGRMYGESELLAVAW
                     AYQDATDYHLRRPPLTDEGPADG"
     gene            complement(2017..3576)
                     /locus_tag="VD788_13710"
     CDS             complement(2017..3576)
                     /locus_tag="VD788_13710"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXV77369.1"
                     /translation="MSPRWCGAVLLSLIVLPRVAHDVHADTIVVGSKNFPENRLLAEM
                     FAQVIEQGTELTVERKLSLAGTQICFAALEVGAIDLYPEYTGTGLVTILRLADAGDAT
                     ETLNVVRREFRARYDLEWFAPLGFENAYELAVPRTLAEQAGLHTISDLVPIAPRLTAG
                     MGYEFIEREDGLVGLRDTYGLRFGEVIGMQQALKYRAAAERRIDVLDVYGTDGRLLVA
                     DLVVLEDDRDFFPPYSAAPLVRGETSRRHPDIARALAVLAGALDERQMRRLNRRMQEE
                     GEAVERVAADALRELGLRPRSSASEAGSTGAGLARYMWRQRAVLLAQTARHLQLTALA
                     LLAGVLVAVPLGMLLTRAPRLAEPLTRAVGVTQTIPSIALLAFMIPLLGVGFRPALVA
                     LWVYSIYPILRNTITALRDASPDAVDAARALGMTDRQILRGVRLPLALPVVLAGIRTS
                     AVLTIGTATLAAFIGAGGLGEPIVSGLQLADGVRILSGAIPAAALAISVDALLGVVER
                     RLTPRGVARVR"
     gene            complement(3573..4319)
                     /locus_tag="VD788_13715"
     CDS             complement(3573..4319)
                     /locus_tag="VD788_13715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXV77370.1"
                     /translation="MDRALQAIRVTKVFPGDVRALRGMDLDVRHGETVALIGESGCGK
                     TTLLRMFNRLLEPSDGEIRIGVDPATAIEPIALRRQTGYVPQDGGLLPHWDVLRNVSL
                     VPVLLGWPRDETRARAEQLLDMVGLPPDRYRRRYPCELSGGQRQRVAFARALAGDPGV
                     VLLDEPFGALDALTRLELQREFLRLKTRLSKTMVLVTHDLNEAFRLADRIAVMRDGRA
                     LQIGSRDELVRRPADRYVAELVALAGAAGR"
     gene            complement(4337..4825)
                     /locus_tag="VD788_13720"
     CDS             complement(4337..4825)
                     /locus_tag="VD788_13720"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXV77371.1"
                     /translation="MARRYWLLKSDPDTFGLRELKASPGRRTVWDGVRNYQARNYLRD
                     EMRIGDGVLFYHSRSQPPAVVATAEVVRAGYPDPTQFDRRAKAHDPHSSRDDPRWYAV
                     DIRLDREFDRPVTLPELRTAAGLERMVLLRKGSRLSVQPVSADEWKIILRLGDRRPPR
                     ER"
     gene            4997..5491
                     /locus_tag="VD788_13725"
     CDS             4997..5491
                     /locus_tag="VD788_13725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV77372.1"
                     /translation="MSAAKSKQPRSNDAAAPSRFSHRAYSIRLRTSLPSTKPALNRGV
                     RAVLKIARACGWVPDQLTDLEIALREALANAIIHGNSLQRGKRVFLRCYARPRRSLMI
                     VVRDEGDGFNPTGVPDPRDDHRLHLPSGRGLLLMRELMDHVEYRRGGREVLLVAHRPA
                     ASES"
     gene            complement(5501..6781)
                     /gene="thrC"
                     /locus_tag="VD788_13730"
     CDS             complement(5501..6781)
                     /gene="thrC"
                     /locus_tag="VD788_13730"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXV77373.1"
                     /translation="MNYRSTRHRVSPAPFSAAMLGGLAPDGGLYVPERFARFERGSIG
                     DEPDFAKIAGLVLRPYLDGDALAGELDEICRTAFDFPVPLEPLRDETAVLELYQGPTS
                     AFKDFGARFLAECTTRRLQAGSPPPPLTVLVATSGDTGGAVAAAFHRRAGIRVAILYP
                     RGGVSERQEQQLACWGDNVLTLAVRGTFDDCQRVVKSAFRDGRLATRHRLTSANSINI
                     GRLLPQMAYYAAGSEGYLARTGHEAGFIVPTGNVGNVAAAFWARASGFPIREIVLATN
                     ANRVIPDWYDRSEWRPRRSVATLANAMDVGDPSNMERLFDLFPHGEGLRQISRAGSVD
                     DDTIRRVIAAGPVSWGRVWCPHTATAVHLRETIGTPHWVVVATAHPAKFDTIVEPLIG
                     RSVEPPPRLAELLERPRQVTEIEAELDALDRALS"
     gene            complement(6778..7752)
                     /locus_tag="VD788_13735"
     CDS             complement(6778..7752)
                     /locus_tag="VD788_13735"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF002288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006566 - threonine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HXV77374.1"
                     /translation="MRRKLDRSRSADGATAFAPGTVANVAVGFDVLGFALVGIGDRVT
                     AKFDPEADRPVTVERVSGVVTDLPTDPERNTASVAVAALLGACQVDAGLRLTLRKGIP
                     LGSGMGGSAASAVAGVVAVNALLDRPLPTAELLPFALAGEAAASGAPHADNAAPSLFG
                     GMTAIVAHDPPHVVDIPLPAGVSCVLVRPALRIDTRDARASLAPAVPLERVVEQSMSL
                     TGFLAGCFRNDVALIGRSFNDLIAGPQRAVRIPGFDDARSAAERAGALGLAIAGSGPS
                     VFAWVPSGAACHAVERAVRDAFARHGLETDGWISPLGCPGARIEREAT"
     gene            7809..8336
                     /locus_tag="VD788_13740"
     CDS             7809..8336
                     /locus_tag="VD788_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77375.1"
                     /translation="MSLLDNLDPETLSPSRLWGEMDPASRRLAVETVYGDAVDDETAR
                     AEVDAAIAAAMRFRTATVRKLPQHKRVDYALRALRPDDTLASTLLLVLHLGRRREILE
                     AFLDHLGIAHERGTIREHALEPPSVERLAAAVDDLYARFAGDQVDLYLAALLAMEPDA
                     WAGLIPALESRKRGA"
     gene            8423..>9504
                     /locus_tag="VD788_13745"
     CDS             8423..>9504
                     /locus_tag="VD788_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HXV77376.1"
                     /translation="MEWSPGDRLVHRFNPELGPGVVRETDGRTVVVEFPETGTTLRLA
                     CDSDALGRLSIRPGIRARLEPSGETVVIEELREPNSALLEDGRLVKTAQLWPLVTGES
                     LPERLAAGQVDPLDAFALRLDLLHLAAIREADGLGSFLGGRIHLFPHQLYVAERATRS
                     DPVRWLLADEVGLGKTVEACLILNHLVRTRRAERTLVVAPETLTVQWLGELWRKYHQV
                     FVLLDDKRLHDVERDYGRGFNPFDAHRSVVVGLERLRGNPWLTERAVEAGIDLLVVDE
                     AHHLRRPPGHPGDASYRAIAPIAALGRHVLLLTATPLEEDAHGFFRLLQLLRPDEFHA
                     EESFDERLRAGRVLPACTSATRRGDIG"
BASE COUNT         1454 a         3336 c         3330 g         1384 t
ORIGIN      
        1 gcctgacgcc cgaggacgta ggccggaccc gggctccggc gcccggtccc gacgcgtacg
       61 ccggcgcgag cgagcaccag cgcggcacgg ggctcgcgga tgccggtcag atagaccaga
      121 ttggggttga ccccctcggc cccaccgccg cgcacgacga tcagcccggc actcacctcg
      181 cgcatcaacc gcgcgcggcg ctccagatag atccggttat ccatctgccg ggccctcgtc
      241 cgtcaacggc ggacgtcgaa gatgatagtc ggtggcgtcc tggtatgccc aggcgaccgc
      301 gagcaactcg ctctcgccgt acatcctccc gttgaaggtg atgctggtcg gcgtcccgtc
      361 cgaggcccgg tgcccgttgg gcagaaccac cgacgggtgc ccggtcaggt tggtcaacaa
      421 caggttgtcg ccgccgaagc tcggacagac gtagacgtcg acaccggcga ggcactgttc
      481 catgtcctgc atcaactgcc agcgtatccg gttggcgcgc aggtactcga cggcgggaat
      541 gaactggccc tgcctgagca cgttgggcca cgcgttggcg acctgccgga cgagctcgcg
      601 gtcgcgccct ccccgcgtca gctcgtcgaa ggcggtcgcc gcctccgcgg tgaggatgaa
      661 cgacagcgcc gccaccggca cgctgtcggg cagttcgatc gggatcagct cgatgccgat
      721 ctcctcgagc ctcgccaagg tgcgccgatc gaagcgcagg gattcctcgg ccagctcgcg
      781 ctcctccggg tcggtgatgt cttccgcgat ctcttgctcg aacagcttgg caacgtagcc
      841 gacccgcagc gagcgcggat ctcgttcgag cggccagtcg aacggacggt cgaccaccgt
      901 cgggtcgagc cagtcgcgcc cgtggatcgc gtcgaagacc agcgcgcagt cctcgacgct
      961 gcgggcgatc ggaccgagct tgtccatgct ccagctcagc gccatcgcgc cgtagcggct
     1021 gactcgcccg aagctcgggc gcagaccggt gacgccacag cgcgagcagg gcgagacgat
     1081 gctgccccac gtctcggaac cgatggcgaa tccgacgagc ccggcggcgg tcgcggccgc
     1141 cggcccggcg gacgatccgc tcgagccctg ctcgacgttc cagggattgc gggtccgacc
     1201 gccgaaccac acgtcgcccc aggccagtgc ccccaacgtc agtttggcgg tcaaaacggc
     1261 accggcgccg gccagacggg agacgacggt ggcctgctcg ctccgcacct ggtccttgta
     1321 cggcgtggca ccccacgtcg tcgggaacgg cggcaccgcg agcaggtcct tggcgcccca
     1381 cggtatccct tgcagcggtc cgcgataggc accggccgcg agctcccggt cggcgcgacg
     1441 cgcctgttcg agcgccagct cggccgtccg ggtgatgacc agctcgagcc gcgggtcgaa
     1501 tcgctcgagg cggtcgaggt agatgccggt cagctcctcg gatgagatct cgcgggtgcg
     1561 gatcagcgcg gacaaccggc tgacgggcag ataggccagt tcgtcgtcgt ccggtcgcgg
     1621 cccgggcagc gacgaaagcg gccgcgcgcg gctctcgttc cgaccttcgg gtgccggatg
     1681 cagcgcctgc ggcgagaata gcaacgccgg tggcaggccg ttgtcgagcg ggatctggcg
     1741 aagctgctcg aagtcggcga cgagctcctg catgccctcg accatcagcg cccgccgctc
     1801 gttgtcgagc tcgattccgg agatccactc ggcctgccgg atcatcccct cgtcgacggt
     1861 ggcgcgatcg gcggcgagcg tcagcagcgc acgactgaac accgcccccg tgccggcggc
     1921 tccgaacagc gccagcatcc tcctgcgtga ccagcctgcc accgcgtggt tcgtcggatt
     1981 cgacatcttg ccctccctcc cgttcggcgt ggaagcttag cgcactcggg cgaccccacg
     2041 aggggtcagc cgacgctcga cgactccgag tagagcgtcg acggaaatgg ccagcgccgc
     2101 cgccgggatc gctcccgaca ggatgcgcac gccgtcggcg agttgcagac cgctgacgat
     2161 cggctccccc agcccgccgg caccgatgaa tgcggcgagc gtcgccgtcc cgatcgtcag
     2221 cacggccgat gtccggatcc cggcgaggac gaccgggagc gccaacggca gccgcacgcc
     2281 gcgcaggatc tgtcgatcgg tcatcccgag cgcccgcgcc gcgtcgacgg cgtcgggcga
     2341 ggcgtcgcgc agggcggtga tcgtgttgcg cagaatgggg tagatcgaat agacccacag
     2401 ggcgaccagt gccggccgga atccgacacc cagcagcggg atcatgaagg ccagcagcgc
     2461 gatcgacggg atcgtctggg tcacgccgac ggcacgcgtc agcggctcgg ccagccgcgg
     2521 cgcccgcgtc agcagcatgc cgagcgggac ggctaccagc acgccggcga gcaacgcgag
     2581 cgcggtcagt tgcaggtgac gcgcggtttg agcgagcagc acggcgcgct gccgccacat
     2641 gtagcgtgcc agacctgccc cggtcgatcc ggcttcgctc gccgacgacc gcgggcggag
     2701 gccgagctcg cgcagggcgt cggcggcgac gcgttcgacc gcttcacctt cctcctgcat
     2761 gcgccggttg agccggcgca tctgtcgctc gtcgagcgcc ccggccagca ccgcgagcgc
     2821 gcgcgcgatg tctggatggc gtcgcgacgt ctcaccccgc accagcggcg cggcggaata
     2881 cggcgggaag aagtcgcgat cgtcctcgag cacgacgagg tcggcgacga gcaggcggcc
     2941 atcggtgccg tagacgtcga gcacgtcgat cctgcgctcg gcggcggcgc ggtacttgag
     3001 cgcctgttgc atgccgatca cctcgccgaa ccgcaaaccg taggtgtcgc gcagcccgac
     3061 cagcccatcc tcgcgctcga tgaactcgta ccccattccg gccgtgagcc gcggtgcgat
     3121 cggtaccagg tccgagatcg tgtgtaaccc ggcttgctcg gccagcgttc gaggcacggc
     3181 caactcgtag gcattctcga atccgagcgg ggcaaaccac tcgagatcgt agcgcgcgcg
     3241 aaactcgcgg cggacgacgt tcaacgtctc ggtcgcgtcc cccgcatctg ccaggcgcag
     3301 gatcgtgacc aaaccggttc cggtgtattc cggatacaga tcgatcgcgc cgacttcgag
     3361 cgcggcgaag cagatctgcg tgccggccag gctcaacttg cgttcgacgg tcagctcggt
     3421 cccttgctcg atcacctggg cgaacatctc ggccagcagg cgattctccg gaaaattctt
     3481 cgaaccgacc acgatcgtat cggcgtgcac gtcgtgcgcg acccgcggca ggacgatcag
     3541 cgacaggagc accgcgccgc accaccgcgg gctcaccgac ctgccgctcc ggccagcgcg
     3601 acgagctcgg cgacgtagcg atcggccggg cggcgcacca gctcgtcgcg ggagccgatc
     3661 tgcagtgctc ggccgtcgcg catgaccgcg atccgatcgg ccagtctgaa cgcctcgttc
     3721 aagtcgtgcg tgacgaggac catcgtcttg ctcagccggg tcttcagccg gaggaactcg
     3781 cgttggagct cgagacgggt caacgcgtcg agcgccccga acggttcgtc gagcaggacc
     3841 actcccgggt cgccggccag tgcccgggcg aacgcgactc gctggcgctg tccgccggaa
     3901 agctcgcagg ggtaacggcg ccgataccga tcgggcggta atccgaccat gtcgagcagc
     3961 tgctccgcgc gcgcacgcgt ttcgtcgcgc ggccagccga gcaacacggg cacgaggctg
     4021 acgtttcgca ggacatccca atgcggcagc agcccgccgt cctgcggcac gtagcccgtc
     4081 tgccggcgca gcgcaatcgg ctcgatcgcc gtggccggat cgaccccgat gcggatctcg
     4141 ccgtcgctcg gctcgagcag tcgattgaac atgcgcaaca gcgtggtctt gccgcagccg
     4201 ctctcaccga tcagggcgac ggtctcgccg tgccggacgt cgaggtccat cccacgcaac
     4261 gctcgcacat cgcccggaaa gaccttggtt acccggatgg cctgcagcgc acgatccatc
     4321 cgttcgccgc ccgacctcag cgctcgcgcg gtggtctgcg atcgccgagt cgaaggatga
     4381 tcttccattc gtcggcgctg accggctgga ctgacaggcg gctgcccttc cgcaacagca
     4441 ccatccgttc gagccccgcg gcagtccgca gttccggcag cgtcaccggt cggtcgaatt
     4501 cgcggtcgag ccggatgtcg acggcgtacc agcgcggatc gtcgcgcgag gagtggggat
     4561 cgtgcgcctt ggcgcgacga tcgaactggg tcgggtcggg gtagccggcg cgcacgacct
     4621 cggccgtggc cacgaccgcc ggcggctggc tccgggaatg gtagaacagc accccgtcgc
     4681 cgatgcgcat ctcgtcgcgc aagtagttgc gggcttggta gttacgaacg ccgtcccaga
     4741 cggtccgccg acccgggctg gccttcagtt ctctcaggcc gaaggtgtcg gggtcggact
     4801 tcagcagcca gtagcgtcgt gccatcgccc gctagattag gccgggcgtt ggtcgatgac
     4861 aagcgcggcg cgccttgaac cccgggggcg gctttccgta caatcgggcg gcgtgccgct
     4921 cgacggcgcc gggagcagga ggtcatgggg attggcggtt gccgttgaag gcgacgcgtg
     4981 cggcgggagc cgcgcgatga gcgccgcgaa gtcgaagcag ccgcggtcga atgacgccgc
     5041 ggcgccgtcg cgcttttctc accgggccta ttcgattcgt ctacggacgt cgcttcccag
     5101 taccaaaccc gcgctcaacc gcggcgtgcg ggccgtgctg aagatcgccc gcgcctgcgg
     5161 ttgggttccg gaccagctga ccgatctcga gatcgccctg cgcgaagcgc tggccaacgc
     5221 gatcattcac ggtaactcgt tgcagcgcgg caagcgggtc ttcctgcgct gctatgcccg
     5281 gccgagaagg tcgctgatga tcgtcgtgcg cgacgagggc gacggcttca acccgaccgg
     5341 tgtccccgac ccgcgcgacg accaccgtct gcatcttccg agcggccgcg gcctgctgct
     5401 gatgcgcgag ctgatggacc acgtcgagta ccgccgagga ggtcgcgaag tgttgctcgt
     5461 cgcgcaccgt ccggcggcgt ccgagtcctg aacggccgat tcacgacagc gcacggtcga
     5521 gcgcatcgag ttcggcctcg atctccgtca cctgccgcgg gcgttcgagc agctcggcca
     5581 atcgcggtgg cggttcgacg gagcggccga ttagcggctc gacgatcgtg tcgaacttcg
     5641 ccggatgtgc ggtcgcgacg acgacccagt gcggtgttcc gatcgtctcg cgcaagtgga
     5701 cggcggtcgc ggtgtgcggg caccacacgc gaccccagga tacgggcccg gcggcgatca
     5761 cgcgccggat cgtgtcgtca tcgaccgagc ctgcgcggga gatctgccgc agtccctctc
     5821 cgtgcggaaa caggtcgaac aaccgctcca tgttgctcgg atcccccacg tccattgcgt
     5881 tcgccagcgt ggccaccgac cggcggggtc gccactccga cctgtcgtac cagtcgggga
     5941 tcacccgatt ggcgttggtc gcgagcacga tctcgcggat cggaaaaccg ctcgctcgcg
     6001 cccagaacgc cgcggcgacg ttgccgacgt tgccggtcgg aacgatgaag ccggcctcgt
     6061 ggccggtgcg ggccaggtac ccctcggagc cggcggcgta gtaggccatc tggggcagca
     6121 gtcgcccgat gttgatgctg ttggccgagg tgagccgatg acgggtcgcg agccgcccgt
     6181 cgcggaacgc gctcttgacg acgcgctggc aatcgtcgaa cgtcccgcgc acggccagcg
     6241 tcaggacgtt gtcaccccag caggctagct gttgctcctg ccgctccgac acgccgccgc
     6301 gtgggtagag gatcgcgaca cggatgccgg cacgacggtg gaacgccgcg gcgacggcgc
     6361 cccccgtgtc cccggaggtc gcgaccagga ccgtcagcgg tggcggtggc gagccggctt
     6421 ggagtcggcg tgtcgtgcac tccgccagga accgcgcacc gaagtccttg aacgcggagg
     6481 tcggcccttg gtacaactcg agcacggcgg tctcgtcccg cagcggctcg agcgggacgg
     6541 ggaagtcgaa cgcggtgcgg cagatctcgt cgagctcgcc cgccagcgcg tccccgtcga
     6601 ggtagggtcg cagcacgagc cccgcgatct tcgcgaagtc gggttcgtcc ccgatcgatc
     6661 ctcgttcgaa gcgggcgaat ctctcgggaa cgtagaggcc gccatcgggc gcgagcccgc
     6721 cgagcatcgc cgcggaaaac ggcgccgggc tgacccggtg gcgcgtgctg cggtagttca
     6781 cgtcgcctcg cgctcgatgc gcgctccggg acaccccagc ggcgagatcc aaccgtcggt
     6841 ctcgagtccg tgccgcgcga acgcgtcgcg caccgcccgt tcgacggcgt ggcaggccgc
     6901 ccccgacggc acccaagcga acacgctcgg cccggaaccg gcgatggcga gtccgagggc
     6961 gccggctcgc tcggccgccg agcgggcgtc gtcgaagccc ggtatgcgca cggcccgctg
     7021 cggaccggcg atcaggtcgt tgaacgagcg accgatcagc gcgacgtcgt tgcggaagca
     7081 gccggcgagg aagccggtca gcgacatcga ctgctcgacg acacgctcga ggggcacggc
     7141 cggcgcgagt gacgcccgcg cgtcacgcgt gtcgatccgg agcgccggac gaaccaggac
     7201 acagctcacg cctgcgggta gcgggatgtc gaccacgtgc ggcgggtcgt gcgcgacgat
     7261 cgcggtcatc ccgccgaaca gcgacggcgc ggcgttgtcc gcgtgcggtg cgccgctcgc
     7321 cgccgcctcg ccggcgaggg cgaacggcag caactccgcc gtcggcagcg gccggtcgag
     7381 cagtgcgttg accgccacca cgccggcgac ggccgacgcc gccgagccac ccatgccgga
     7441 gccgagcgga atacccttcc gcagcgtcag tcgcaggccc gcatcgactt ggcaggcgcc
     7501 gagcaacgcc gcgacggcga cggaggccgt gttccgctcg gggtcggtcg gcaggtcggt
     7561 gacgacgccc gacacccgct cgacggtcac cggccggtcc gcctcggggt cgaacttcgc
     7621 cgtgacgcgg tcgccgatcc cgacgagggc gaaaccgagc acgtcgaagc cgactgcgac
     7681 gttggcgacg gtgcccggcg cgaatgccgt ggccccgtcg gcggaacgcg atcgatcgag
     7741 cttccgacgc atgatgctgc tcgctagaat agacgctcgg cggaacagat caagccgagg
     7801 agtcgccgat gtcgttgctc gacaacctgg acccggaaac gctgtcgccg tcgcggctct
     7861 ggggcgagat ggaccccgcg tcccgccggc tggcggtcga aacggtctac ggcgacgccg
     7921 tcgacgacga aacggcgcgg gccgaggtcg acgcggcgat cgccgcggcg atgcgcttcc
     7981 gaacggctac cgtgcgcaag ctcccgcagc acaagcgggt ggattacgcg ctgcgcgccc
     8041 tgcgccccga cgacacgctg gcgtcgacgc tgctgctcgt gctgcatctc ggccgacgcc
     8101 gggagatcct cgaggcgttc ctcgaccatc tgggcatcgc gcacgagcgc gggacgatcc
     8161 gcgagcacgc gctcgagccg ccgagcgtcg agcgcctggc ggcggcggtc gacgatctgt
     8221 acgcccgctt cgccggcgat caggtcgatc tttatctcgc cgcgctgctg gcgatggagc
     8281 ccgacgcctg ggccgggttg atcccggcgc tcgagagccg taagcgcggc gcctgatctc
     8341 cactcgactt cgcccgtgca ccgggattgc gatcggcgcg ggccagcctc tagtatcggc
     8401 agccacgacg ggagccgacc cgatggaatg gagtccgggc gacagactgg tccatcgctt
     8461 caacccggaa ctgggaccgg gtgtcgtgcg cgaaaccgac gggcgcaccg tggtggtcga
     8521 gtttcccgag accgggacga cgctgcgcct ggcctgcgac tcggacgccc tgggacggct
     8581 gtcgatccga ccgggaatcc gggcgcggct cgaaccttcg ggagagaccg tcgtcatcga
     8641 ggagctgcgc gagccgaaca gcgcgttgct cgaggacggc cggctggtca agacggcgca
     8701 gctatggccg ctcgtgacgg gagagtcgct gcccgagcgg ttggccgcgg gccaggtcga
     8761 tccgctcgac gcgttcgcgt tgcgactcga tctactgcac ctggcggcga tccgcgaggc
     8821 ggacgggctg gggtcgttcc tcggtggccg gatccacctg ttcccgcacc aactgtacgt
     8881 cgccgagcgc gcgacgcggt cggacccggt ccgctggttg ctggccgacg aggtcgggct
     8941 gggcaaaacg gtcgaggcgt gcctgatcct caaccacctg gtacgaacgc ggcgagccga
     9001 gcggacgctg gtcgtggcgc ccgagacgct gaccgtccag tggctcggcg agctgtggcg
     9061 caagtaccac caggtcttcg tgctgctcga cgacaagcgg ctgcacgacg tcgagcgtga
     9121 ctatggccgc ggcttcaatc cgttcgacgc gcaccgctcg gtcgtcgtcg gcctcgagcg
     9181 gctgcgcggc aacccgtggc tgaccgagag ggccgtcgaa gccggcatcg acctgctggt
     9241 cgtcgacgag gcccaccacc tgcgccggcc gccgggtcac ccgggcgacg cgagctatcg
     9301 ggcgatcgcg ccgatcgccg cgctcgggcg acatgtcttg ctgctgacgg caaccccgct
     9361 cgaggaagat gcccacggtt tctttcgctt gctgcagttg ttgcggcccg acgagttcca
     9421 cgcggaggag agcttcgacg agcggctgcg cgcgggacgg gtgttgccgg cctgtaccag
     9481 cgcgacccgt cgcggggaca tcgg
//
LOCUS       DASPGN010000473        23305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1258139, whole genome shotgun sequence.
ACCESSION   DASPGN010000473 DASPGN010000000
VERSION     DASPGN010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 23305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23305
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258139"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2484
                     /locus_tag="VD788_13750"
     CDS             <1..2484
                     /locus_tag="VD788_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV77377.1"
                     /translation="ERLTLEIEPLWNPRPEPYLYLSIVGLAFWVSGLFIAIKWPLIRG
                     GAVYGLFSMAMFAQLILSHSGRADVLDAAINWGDLIAGALAPALLLHLGVVLSKGVVN
                     RRVPWVAATYGVATIAMMSAVWIRPDAAGGAYRFSDPLLMVEAHQRLGPLWLSVCWLS
                     TTVLLIRSFHNSSSVMHRSQLRWVLWGLVVGIGPFVLFYAVPWTLGAPELPAWAEFLA
                     IAPMIVVPGAFATALARYRLHDLDLILLRGITEASAVFGTFAVLAATIFILREGLAGF
                     LPLSRSATRYVGFLVAAVSYPQMRAWARAGIERAFYRRRYSYRTTLLDWARELSAETD
                     LWSLLTRLRERVRDTLGIDHAAVLLRIEDNRFDLCADRLRSCTTEIDAGLAERLETQP
                     YVTFEAGTLPDLPWASYLFSIRVKDQLRAVLAVCERRQPEDPLNTEDRALLGTLAAHA
                     ATAIEAARLFLEVRERADEIGRLHATQAKILESSAVGLLLLDGQGKIQAWNRALEAIY
                     GLERREAIGRGLREVFPFHVVRRLEREAGLNDSVEEARLFRLSMVNHAGRRILANVSI
                     SPADGETGDDGSRVITFDDVTEQVELEDQVLRQERLASLGLLAAGVAHEINTPLTGIS
                     SYTQLLMDELDPDDRRRAVLEKIEQQTQRAAGITGSLLNLARPANTAAEPIDVNRLIG
                     EVIQLFEPQVRRTGIRLTTSLSDGLPRIAGNRGKLQQVVLNLLINARDALGQRGTIAV
                     ATRGVAGGGVAIDVTDDGPGIADDDLPRIFDPFFTTKGRSKGTGLGLSISYGIVREHD
                     GQIHVESRPDRFTRFRVELPAIRSAEAIG"
     gene            2481..3893
                     /locus_tag="VD788_13755"
     CDS             2481..3893
                     /locus_tag="VD788_13755"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV77378.1"
                     /translation="MSPTTPQAGRAAPVADAAARVLVIDDEPVVVEVLRELLARTGYR
                     VEVASDGFGGRGALEEGGWDALLLDVMLPDEDGLHLLRWVRERQPDLAVVMITAFGTV
                     ENAVQSMKSGAFHYVTKPFKNDEVRHLVAQAIQTTRLRRENRNLRRSLEARDRFERIV
                     GRSRPMQEVYSFIDQVAPSRSTVLIQGESGTGKELVAHAIHSRSPRADKPFVVVNSSS
                     IPADLLEDNLFGHERGAFTGADSAKIGLLEAADGGTVLFDEITTVAPAVQAKLLRVIQ
                     EKAFLPLGSIKTRTVDVRILAATNEDVRRLVDDGRFREDLFYRLNVLLITLPPLRQRL
                     DDLPLLAGHFLRHYNRENAKRVDAISPEALDRMLAHRWPGNVRELENVIERGVVLAHG
                     EEITVDLLPRELQTGGTTPAPVRLPDGLRFYDAVSRFERQLIEAALRRSGGIQKQAAE
                     MLGLKPTTLNEKIKRLRIRG"
     gene            4221..7409
                     /locus_tag="VD788_13760"
     CDS             4221..7409
                     /locus_tag="VD788_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV77379.1"
                     /translation="MRIVRAVIATFSVAAAISVVSAQSGGIEVTVLDSEGQPLPAATV
                     TISHPTGYVKETAQLTNKDGVVAFPVLRATGSSVGYNINVAFPGFASMQLTDIKVNIG
                     RTNNLPIQLQAEITETVTVTATTDVVDLENTEQTTKFTDEFIQDLPVPGRFYQNVLTL
                     APGVQDPDGDGNPNVHGSRDRDFKAEVSGMSNVDPLTGQQMSQVNPNSIEEMEVITAG
                     AGVEFSRAQGGFARILQKQGSNDLEGVFEMYYSSSLLDGTGAQDFNNTTDPDFDSFQP
                     GFQLSGPIVKDKLWYRLSHQYIKREDPTNTVSGLEVVTDEWGIHSDAITWQVSPRNKL
                     QFQIQLDPRTITNFGVTSTRPAEAAQIREFGSDTYSLTWTAPYSPKILIQSQVGYQER
                     NLDISPTSATATNNCVTAPDLAFLETAQCFNTQTGEWSGSFNQTWNDARKRLTFRGDT
                     TVYAGRFLGATHQFKFGMSIENENYTRDLYQRPVVFYSQLTFQDPTRPQDDPVPKILI
                     DGTFAAPDYTSINAKGVNWGFYAEDQFKPAQNLTVTLGVRIDREEIRSNGKVPIDPEA
                     EFQNYLGLVEAGDPVNAHARSFTAYEDPREFVRTLASQLGIDFNTLWGSLSNASTQSE
                     FWPTSRANGNVDVVNTNPAPRLALSWDPWSNGKTKVFATAGRYYDKIFLGVPLIELNP
                     PTTNLNFESFIDPNNGRRVVQLNEAVKAAVNVNTVDRDLETPYNDEWTLGFEREIATE
                     TSLRVTYVNRRFRNQLQDLDLNHAPGDYGRDCNPRGEPTFVVPVQPADPGYDPRYAPG
                     DGVYDDCVGRIEFDRIQSEVVGGNGGAQQDEAIGTLEFPDGFADLYGLNPGWGDMYLV
                     GNLNRIDYEGLTIELIRRQYRSWEMTGSYTLSEAFGDGEDFQQNLGDDRSTIEDEQGY
                     QAYDRRHAVKVAATSITPWGFRLGANLTWQSGLPYSLLTRQVSFDAAAPPHGTLAPVG
                     VRRQRTTYDTGQRNDQRNDSFWNLDLKLTKEMNVGRKLNMQLSAEIFNALADDTYTIY
                     DPINQVGEQVNGVNTAFRRFGRRWQLGVRFSF"
     gene            7591..10758
                     /locus_tag="VD788_13765"
     CDS             7591..10758
                     /locus_tag="VD788_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV77380.1"
                     /translation="MGERTAVIVLFAAALLLTASPARAQSGAIAVEVLDAEGRPLPGV
                     TVEIGHPTGYVKRTAVLTDADGHADFPVLRATGAVGPGYELTIRLAGFSTVRLPDVKV
                     RVGETNRFPVRLTTQLEERVRVEGGSEVVDLEDTTQTTKFSDEFMQDLPVAGRFYQNV
                     LTLAPGVQDADGDGNPNVHGSRDRDFKAEVSGVSNVDPLTGQQMSQINPNSIEEMEVI
                     TAGAGVEFSRAQGGFARILQKQGSNDLEGVVEFYIRSSVLDGTGADDSNNTTQPDFAS
                     YQPAFQLSGPIVRDRLWYRLSHEWIYTEDPQNTTRGISIVEDRWAIHSDQLTWQVSPR
                     NKLAFQYQADPRTIDNFGVNSTRPEEAALFIENESESYALTWTAPLSTRILIDSRVAW
                     QDFNVTVAPTEVGVENRCLTGPAFLERAQCFNLLTGERSGSYPQDWNDHRQRLTVRGD
                     ATIYGGRFLGADHQLKIGAIVENERYTRELERRPTVSFTVINSTDQTNTDEDSPERVA
                     VISGSFSVPEFTEVTARGATWGLYAEDRFRPATNLTLTLGARVDREEIRSAGREPLDP
                     QGEAAEYLSLVESGTSAQMAAPAVFTAYEDPRDFFRQLAAMLEIDFNELFLSQSPMAV
                     QSEFWQNSRHPGSIDLANTNFAPRFSLAWDPGRDGKTKLAVTAGRYYDKLFLNIPLIE
                     LEPASTDLVFEAFPRGLDWQVTGLREAVNPALNVDAVDRTLDTPYQDEWTVSFERELA
                     LETSARVTYVNRRYRDQLQDIDINHEPDDYGRCVTASPSNPRLTVTPILPSDPTYDPA
                     VAPGDGVVDDCIGRLVVINDPESPGGSVLLPRPDGTADLYVQNPGWGDVLLVGNQNRI
                     DYEAVVFELTRRQYRNWEMQGSYTWSAARGDGEDFSQSLGDDRSLVSDEQGFQAYDQR
                     HVVKFNATTITPWGFRLGGAISWQSGLPYSLLTRRQAFDAVPPQYGTLGSAAVRPRTT
                     YPSGTRNDQRNDSYWNVDVKLTKELRVGRGVGLQLSAEVFNLLNDGTYLIYDPVSQTG
                     EQINGNNVAIRRFGRRWQLGARVAF"
     gene            10827..12224
                     /gene="cysS"
                     /locus_tag="VD788_13770"
     CDS             10827..12224
                     /gene="cysS"
                     /locus_tag="VD788_13770"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXV77381.1"
                     /translation="MKIYNTLTRRLEGFEPLVPGKVSMYTCGPTVYDFAHIGNFRAYV
                     WEDLLRRTFEHLGFEVTQVMNITDIEDKIIGKMAELDLDLDSVVQPFVDAFFEDLATL
                     RIRRAEHYPRATEHIAEMTQLALELRDRGLTYESQGSLYFKIAAFAGYGRLSNLDNRR
                     IKPGARVDSDEYDKEDVRDFVLWKGGKPGEPTWDSPFGPGRPGWHLECSAMSMKYLGQ
                     TFDLHTGGVDNIFPHHENEIAQSEGATGRPFVRYWMHVAHLMVDGQKMSKSKGNFYTL
                     RDLLARGFDPRAIRLLLLATHYRTPLNFTLDGLSQSTAEVGRLESLVGRLDREPEGGG
                     RNPSFDEAIRRAERVFGESLTDDLNISGALGAVFGLVREANVALDRGELPRTSRAGLR
                     VALERFDGVLDVFRPAATGYAVDVDDYIKRRDEARKARNWAEADRIREELSAAGIVLE
                     DTPHGTIWRRGLKSR"
     gene            12340..12780
                     /locus_tag="VD788_13775"
     CDS             12340..12780
                     /locus_tag="VD788_13775"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXV77382.1"
                     /translation="MGPRRSRRESSSDDPRQRLGSDGERAAERVLREAGLSIVERRFR
                     LKIGEIDLIASDGPLVVFVEVKTRRGSSHGLPAQAVTPRKRATIARVAAAYLVRRGLS
                     ERPCRFDVVQVFAGRRGVERVDHIRDAFRLWDDDRSTRFTRRLG"
     gene            12866..12941
                     /locus_tag="VD788_13780"
     tRNA            12866..12941
                     /locus_tag="VD788_13780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12899..12901,aa:Gly,seq:gcc)
     gene            12977..13576
                     /locus_tag="VD788_13785"
     CDS             12977..13576
                     /locus_tag="VD788_13785"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXV77383.1"
                     /translation="MRTRSWLVFALSIGVAGLVYAARPMALTNGDPAPPMRGNLPNGD
                     LLIVDYSPTELTLINFWATWCEPCRKEMPALDDLYRRHAGAGLQVIGVHVGPLTDDEY
                     AQFKEAVPVDYPLILNPSARWLSPWGGLSILPRSYLVASDGRILRGYVGASAEQVEAL
                     VYDVEAVLDGRPLGPVVVPTEPSDVSTPEDRDRAKDETE"
     gene            complement(13588..14511)
                     /gene="lipA"
                     /locus_tag="VD788_13790"
     CDS             complement(13588..14511)
                     /gene="lipA"
                     /locus_tag="VD788_13790"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXV77384.1"
                     /translation="MANERGNRPRPDWLKIRLRTDDGFRGLRSMVRELRLNTVCTEAR
                     CPNIYECWNAGTATFMILGDTCTRRCGFCSVTSGLPRPGVDPDEPRNVAEAVSRLRLR
                     HAVITSVDRDDLDDGGARHFADVIAAIRRRSPSCAVEVLTPDFKNKRGAPELVLRAAP
                     EVFAHNVETVPRLYRTARPGSRYRGSVELLASAAAWRERMQPALRVKSSLMLGLGEAD
                     DEVVAVMRELKQAGVDVVTLGQYLQPTKEHLPVARFVSPAEFDRFRGIGAELGFLHVE
                     SGPLVRSSYHAERHRPDPAPDAGSHRLRVVG"
     gene            complement(14514..15218)
                     /gene="lipB"
                     /locus_tag="VD788_13795"
     CDS             complement(14514..15218)
                     /gene="lipB"
                     /locus_tag="VD788_13795"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HXV77385.1"
                     /translation="MQPLLVRWLGQVGYAAGVVLQDRLVARRRHDEVPDTLLLLEHEP
                     VITCGRGADMAHLLVDRETLARRGIEVHESGRGGDVTYHGPGQLVGYPVLALAPERRD
                     AHRYLRELEQVLIDAVAELGVTGRRVEGLTGVWVGERKLAAIGVRLSTGWITSHGFAL
                     NVGTDLDGFRAIVPCGLHGRGVTSLAECCGVEPAMPEVAWRVVRSFCRVFGRRPVASP
                     RPDERLGKPLDGEVCS"
     gene            complement(15233..16495)
                     /gene="sucB"
                     /locus_tag="VD788_13800"
     CDS             complement(15233..16495)
                     /gene="sucB"
                     /locus_tag="VD788_13800"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR01347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HXV77386.1"
                     /translation="MRVDVTMPQMGESIAEGTITRWIKRVGEKVERDEPIFEISTDKV
                     DAEIPAPSAGVLVEIRSAEGDTVPVNDVVAVIETNGEPSGDDAAGGRTGAAQASSAAA
                     PAAGRETAAAAGSRFASPVVRKLAAEHGVDPGRVRGSGAGGRVTKKDMLAFLESRSGA
                     ADAPPSLTARPAAAAPAAVAPRAATPEFGSRSVVRERMSVMRKKIAEHMIESRRTSAH
                     VHSVFEVDMTAAMQLRARHRQSFVERHGVKLTVTPFFIKAACDALEAWPIVNASVDGD
                     EIVYHRELNIGVAVALDWGLIVPVLRNANELSIAGLAKRLQDLADRARTKKLNPDEVQ
                     GGTFTITNPGQFGGLYGLPIINQPQVAIMGVGGIEKRPVVIDEAIAIRSMCYLSMSYD
                     HRVVDGAVADQFLAMVKRGLESADESLL"
     gene            complement(16547..17959)
                     /gene="lpdA"
                     /locus_tag="VD788_13805"
     CDS             complement(16547..17959)
                     /gene="lpdA"
                     /locus_tag="VD788_13805"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXV77387.1"
                     /translation="MSARREGRYDITIIGSGPGGYVAAVRAAQLGMRTAVVEKDAAPG
                     GTCLHLGCIPAKALLRTAEVLDTARRADSFGVKIGKAELDVSAMHAYRKKTIDANVRG
                     VEYLFKKNSVTLLRGVGSLAGQGRVRVSPPQDSPYEIESGHVVLATGSVIRELPGTEF
                     DGKRVINSDDALALERIPKSMIVLGAGAVGVELASAYRSFGSEVTIVEMLPRLVPLED
                     EELGKELERAFRKRKIASHTSTRVTAVETDPQGVKLVAEADGKRLELDAEVLLVAIGR
                     RPRTEGLGLDGTPIQLDARGFIEVDEMMRTAMAGVYAIGDLLATQALAHVASHEGIVA
                     VEHAAGADPRPIDYDRIPSCTYCHPEVASVGLSERQAVERGINVGVGKFPFTAIGKAK
                     ILGDTTGFVKIVTDKDYDEVVGVHIIGPHATELIAEATTAVSLEATAESLFHAVHAHP
                     TLSEAMGEAALAVHDRAIHL"
     gene            18213..18629
                     /locus_tag="VD788_13810"
     CDS             18213..18629
                     /locus_tag="VD788_13810"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 family protein"
                     /protein_id="HXV77388.1"
                     /translation="MPDAPRWYTPRTARRVLRRIRPTVELLHRLWVELDYRRPDPIGS
                     DLAVDPLYFVMARRLAHGLAALEARGLLVDELRDGRLDFPAWLDDRPVVLCWRVGEPE
                     LRFWHVRGNGRPRVPFRDGDRFRPPGAPRRRPYSLC"
     gene            18663..19208
                     /gene="nuoB"
                     /locus_tag="VD788_13815"
     CDS             18663..19208
                     /gene="nuoB"
                     /locus_tag="VD788_13815"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HXV77389.1"
                     /translation="MSRPDPKLVLHQRPDGEQEIRQTAYTTRIQDFINWGRKNSLWPM
                     PMGLACCAIELMATVGPRYDLARFGAEALRFSPRQADLMVVSGTVTKKMAPAVRKIYD
                     QMPNPRWVISMGACASSGGMYRSYSVVQGVDEILPVDVYVSGCPPRPEALLEAVMEIQ
                     RKLTRREKSASELAGARPLPV"
     gene            19229..20377
                     /locus_tag="VD788_13820"
     CDS             19229..20377
                     /locus_tag="VD788_13820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXV77390.1"
                     /translation="MTREQPTGDPRLHALVLDEIRSTGPMSFARFMELALYHPTHGYY
                     AAGASRLGPGGDFITASDVGRAFGRAMARQIDQIDRVVGPFHPFSIVEYGAGRGMLAA
                     DVLDALRDEAPGLYERTEYVLVDRSEKMREAARVRVPRAQVVAPEVAGGGRRGVVIAV
                     ELFDALPVHRVRRRGGRLVELRVDADPDGLFVERETPADETVRDWAARFGAANDEGSE
                     AEVSLSAEPTFDRMASAVERGAMIVVDYGDRSPALYGDTRRRGTLLAYHRHATHEDCL
                     ARVGSQDLTAHVNFSVLEERAVELGWDVLALTTQDRFLVANGILEEFEQRDLAESHDP
                     ARVKRRLQALQLIHPEGMGRIFRFLLLSKGAHPPPALAGAADPFGRGR"
     gene            20399..21319
                     /locus_tag="VD788_13825"
     CDS             20399..21319
                     /locus_tag="VD788_13825"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXV77391.1"
                     /translation="MVRDQSPIDPHAIEQAFLDQGFVCTGEVATTLYLAERLAKPVLL
                     EGPPGVGKTEIGKLWAAYHAARLIRLQCYEGLDESKALYEWAYGKQMLYTQLLREKTG
                     ELLAGATDLRDAIKRLASQTDAFFSREFLLERPLLAAIASPDPVVLLIDEIDRADEEF
                     EAFLLEVLSDYQISIPELGTIRARRPPRVILTSNDSRDLADALRRRCLYLYVDYPGHD
                     DELRIIERRAPALGQTLAQNAVRFAQSLRKAELRKQPGVAEVIDWALALVALGARDLS
                     PRILRETLGALLKNRDDLERVLSEVGRTRR"
     gene            21316..22548
                     /locus_tag="VD788_13830"
     CDS             21316..22548
                     /locus_tag="VD788_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV77392.1"
                     /translation="MIEALQRFFEVLHDGGVRSSPAERLDALRALECVGVADRARVKQ
                     ALRATLVKHAAQRGLFDTTFDRFFAAPGRARRGRRRRAGAKGGEVKRTADASAAAAGA
                     PRGREPRHPERDRRSPEALRRALAELRDGRRERWGRLRQVAVRAPREREPDASRPRGR
                     TALVSLRARPEANRRNEIERELRRLIERIRMGAARRLERASRGRPFPRRLFRDSLASD
                     GVPFRIPRRRRRTRTPRVWLLIDVSWSTAHAAGLFLEMASEFVRLGRQCRVIFFVDRP
                     VDATEHVEDWIARGDLAAFVDRLRTVPGVNPDAPSDYGRTFHRLLVSPRRPRGRRSLL
                     VVLGDGRNNEFDPQAWAFAELADGCGATLWLVPEPIEAWGGGDSALAEYLSSVDTVVE
                     ADDLDGLARGVARLVRRL"
     gene            22545..>23305
                     /locus_tag="VD788_13835"
     CDS             22545..>23305
                     /locus_tag="VD788_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77393.1"
                     /translation="MIPFPGALVAVWEAGELTLGVVAGEEKRRLRLVLEGGREIRVQP
                     ARIAWEVEAPGKVPGAAPEERREAGERVERAERRLRGVADSIDVELVWQILAEAGAPP
                     ADGFETGDLAELALDSRSGESMAATVRALRRDGLRFTRKGERWLARAAEQVADLERER
                     ERIAERELQTRALFDALAAAVRGQAFHETGSEYERRYLDALTRLAVDAESAPEVARAL
                     AVEALSASGLRCERPHEGAFRLLRLVGRLEHDDVNL"
BASE COUNT         3668 a         8048 c         7965 g         3624 t
ORIGIN      
        1 gagcgcctga cgctcgagat cgagccgctg tggaaccccc gccccgaacc ctacctgtac
       61 ctgtcgatcg tcggtctggc gttctgggtg tcgggcctgt tcatcgcgat caagtggccg
      121 ttgatccgcg gcggcgcggt ctacggtctg ttctcgatgg cgatgttcgc gcagctgatt
      181 ctctctcatt cgggtcgcgc cgacgtgctc gacgcggcga tcaattgggg ggacctgatc
      241 gcgggggcgc tggcgccggc gctgttgctg cacctcggcg tcgttctgtc gaaaggcgtc
      301 gtcaaccggc gtgtcccgtg ggtcgcggcg acctatggcg tggcgacgat cgccatgatg
      361 tcggcggtct ggatccgacc cgacgcggcc ggcggggcct atcgcttcag cgatccactg
      421 ctgatggtcg aagcgcacca acgtctcggc ccgctgtggc tctccgtctg ctggctgtcg
      481 acgaccgtgc tgttgatccg ttcgttccac aattcgtcgt cggtgatgca ccgcagccag
      541 ctccgatggg tgttgtgggg cctggtcgtc gggatcggtc cattcgtcct gttctacgcc
      601 gttccctgga cactcggcgc tcccgagctg cctgcgtggg ccgagttcct cgcgatcgcc
      661 ccgatgatcg tcgtccccgg ggctttcgcc accgcgctcg cgcgctatcg gctgcacgac
      721 ctcgacctga tcctgttgcg cgggatcacc gaggcgtcgg ccgtcttcgg cacgttcgcc
      781 gtgctggcgg cgacgatctt catccttcgt gagggtctcg ccgggttcct gccgctgtcg
      841 cgcagcgcga cacgctacgt cggattcctc gtcgccgccg tctcctaccc gcagatgcgg
      901 gcctgggccc gcgccggcat cgagcgcgcg ttctaccgcc ggcgttacag ctaccggacg
      961 acgctgctcg actgggcgcg agagctgagc gccgagaccg atctgtggtc gctgttgacc
     1021 cgcttgcgcg agcgggttcg cgacacgctc ggcatcgacc acgcggcggt tttgctgcga
     1081 atcgaggaca accggttcga cttgtgcgcc gaccggctgc ggtcttgcac gaccgagatc
     1141 gacgcgggcc tggccgagcg gctcgagacc caaccctacg tcaccttcga ggccggcacg
     1201 ctgcccgacc taccgtgggc cagctacctc ttctcgatcc gggtcaagga ccagctgcgc
     1261 gcggtgctgg ccgtctgtga gcggcgtcag cccgaggacc cgctcaacac cgaagatcgc
     1321 gcgctgctcg gcacgttggc cgcgcatgcc gcgaccgcga tcgaggccgc gcgtctgttc
     1381 ctcgaggttc gcgagcgagc cgacgagatc ggtcgcctgc acgccacgca ggccaagatc
     1441 ctcgagagca gcgcggtcgg gctgttgctg ctcgacgggc agggcaagat ccaggcctgg
     1501 aaccgagcgc tcgaggcgat ctacgggctc gagcgccggg aggcgatcgg gcgcggtctg
     1561 cgcgaagttt tcccgttcca cgtcgtgcgc cgcctggaac gcgaggccgg gctgaacgac
     1621 tcggtcgagg aggcccgcct gttccgcctg tcgatggtca accacgccgg caggcggatc
     1681 ctggccaacg tctcgatctc gccggcggac ggcgagacgg gcgacgacgg ctcgcgcgtg
     1741 atcaccttcg acgacgtcac cgagcaggtc gagctcgagg atcaggtgct gcgacaggag
     1801 cgcttggctt ccctcggtct gctcgccgcc ggcgtggcgc acgaaatcaa cacgccgttg
     1861 accggcatct cgagctacac gcagctgctg atggacgagc tcgatcccga cgaccggcgt
     1921 cgcgccgtgc tcgagaagat cgagcagcag acgcagcgcg cggcggggat caccgggtcg
     1981 ctgttgaacc tggcccggcc ggccaacacc gccgccgagc cgatcgacgt caatcggttg
     2041 atcggcgagg tgattcaact gttcgagccc caggtgcggc ggaccgggat ccgcctgacc
     2101 acgtcgctgt ccgacgggct gccgcggatc gcgggcaacc gcggcaagct gcagcaggtc
     2161 gtgctcaacc tgttgatcaa cgctcgcgac gcgctcggcc agcggggcac gatcgccgtc
     2221 gcgacgcgcg gggtcgccgg ggggggggtc gcgatcgacg tgaccgacga cgggccgggg
     2281 atcgccgacg acgacctgcc gcggatcttc gacccgttct tcacgacgaa gggccgttcc
     2341 aagggcaccg gtctcgggtt gtcgatcagc tacggaatcg tccgcgagca tgacggccag
     2401 atccacgtcg agagccggcc cgaccgcttc acccgcttcc gcgtcgagtt gccggcgatc
     2461 aggtcggcgg aggcgatcgg gtgagcccga cgacgccgca ggccggccgc gccgcgcccg
     2521 tcgccgacgc cgcggcgcgc gtcctggtga tcgacgacga gccggtcgtc gtcgaggtgt
     2581 tgcgtgagct gctcgcgcgg accggctacc gggtcgaggt ggcgagcgac gggttcggcg
     2641 gtcgcggggc gctcgaggag ggcggctggg acgcgctgct gctcgacgtg atgctgcccg
     2701 acgaggacgg cctgcacctg ttgcgctggg tgcgcgaacg ccagcccgac ctggcggtgg
     2761 tgatgatcac cgccttcggc acggtcgaga acgccgtcca gtcgatgaaa tcgggtgcgt
     2821 tccactacgt caccaagccg ttcaagaacg acgaggtccg tcacctggtg gcacaggcga
     2881 tccagaccac ccgcctgcgg cgggagaacc gcaacctgcg ccgctcgctc gaggcccgcg
     2941 atcgcttcga gcgcatcgtc ggccggtccc gcccgatgca ggaggtctac tccttcatcg
     3001 accaggtcgc gccgagccgt tcgaccgtgt tgatccaggg agagtcgggt accggcaagg
     3061 agttggtcgc gcacgcgatc cacagccgca gcccacgggc cgacaagccg ttcgtcgtcg
     3121 tcaacagcag cagcattccg gccgacctgc tcgaggacaa cctgttcggg cacgagcggg
     3181 gcgccttcac cggcgccgac agtgccaaga tcggcctgct cgaggccgcg gacggcggaa
     3241 ccgtcttgtt cgacgagatc acgaccgtcg caccggcggt acaggccaag ctcctacggg
     3301 tgatccagga gaaggccttt ctcccgctgg gctcgatcaa gacccgaacg gtcgacgtgc
     3361 ggatcctggc cgcgacgaac gaggacgtcc gccgactggt ggacgacgga cggttccgcg
     3421 aggacctgtt ctaccggctg aacgtgctgt tgatcacgct gccgccgctc agacagcgtc
     3481 tcgacgacct gccgctgttg gccgggcact tcctgcgcca ttacaaccgg gagaacgcca
     3541 agcgggtcga cgcgatctcg ccggaggcgc tcgaccggat gctggcccat cggtggccgg
     3601 gcaacgtgcg ggagctcgag aacgtcatcg agcgcggcgt cgtgctggcg catggcgagg
     3661 agatcacggt cgacctgctg ccccgcgagc tgcagaccgg cgggacgacg ccggccccgg
     3721 tccggctccc cgacggcctg cggttctacg acgccgtttc gcggttcgag cggcagctga
     3781 tcgaggcggc cctgcggcgc agcggtggga tccagaaaca ggccgccgag atgctcggtc
     3841 tcaagccgac gacgctcaat gagaagatca aacggctgcg gatccgcggc tagagcccgg
     3901 agacgaagat cttcctggtc ccgaccgacg ttctgcggta aggttcccat cctcccgccg
     3961 atccgcctga gaacctcgaa accgggagtt tcgggcgacg ctgcataact gcagcatcgg
     4021 ttgggtgggc gggggcacac cggaagtaaa aacggatatg tattgtctct ggaggccgcc
     4081 ggtccgaccg aacggcgccc ccggcagagg gtcgcgtcgt gccgcgcacg taccgcccag
     4141 ccggggccgg tctccgacgt cagggggggg ccgccaccgg gtggcgggag aggggccatg
     4201 aggacaagca ggagggcgtc atgaggatcg tacgagcggt catcgctaca ttctccgtag
     4261 ctgccgcgat ctcggtcgta tcggcccagt cgggcggaat cgaagtcacc gtgctggact
     4321 ccgagggtca gccgttgccc gccgcgacgg tcacgatcag ccatccgacc ggctacgtca
     4381 aggagacggc gcagctgacc aacaaagacg gagtcgtcgc cttcccggtg cttcgcgcga
     4441 ccggctcgag cgtgggctac aacatcaacg tcgcctttcc gggctttgcc tcgatgcaac
     4501 tgaccgacat caaggtcaac atcggtcgca cgaacaacct gccgatccag ctccaggccg
     4561 agatcaccga gacggtcacc gttaccgcga cgacggacgt cgtcgatctc gagaacacgg
     4621 agcaaacgac caagttcacc gacgagttca tccaggacct gccggtgccg ggccgcttct
     4681 atcagaacgt cttgacgctc gcccccggcg tacaagaccc ggacggcgac ggcaacccga
     4741 acgtccacgg atcgcgtgac cgcgacttca aggccgaggt cagcggcatg tcgaacgtcg
     4801 acccgctgac cggccagcag atgagccagg tcaacccgaa ctcgatcgaa gagatggaag
     4861 tgatcaccgc cggggccggg gtcgaattct cgcgcgccca gggcggtttc gcccgcatcc
     4921 tgcaaaagca gggcagcaac gacctcgagg gcgtgttcga gatgtactac tcgtcttcgt
     4981 tgctcgacgg cacgggcgcg caggatttca acaacaccac cgaccccgat ttcgactcgt
     5041 tccagcccgg gttccagctc tccggcccga tcgtcaagga caagctgtgg taccggctgt
     5101 cgcatcagta catcaagcgc gaagacccga ccaacacggt ctcgggtctc gaggtcgtca
     5161 ccgacgagtg gggcattcac tccgacgcga tcacctggca ggtctcgccg cggaacaagc
     5221 tccagttcca gatccagctc gatccccgaa cgatcaccaa cttcggcgtg acctcgacgc
     5281 gaccggcgga agcggcccag atccgtgagt tcggctcgga cacctactcg ttgacctgga
     5341 ccgcgccgta ctcgcccaag atcctgatcc agagccaggt cggctaccaa gagcgcaatc
     5401 tcgacatctc gccgacctcg gcgacggcga cgaacaactg cgtcaccgcg ccggatctcg
     5461 cgtttctcga gacggcccaa tgcttcaata cgcagaccgg cgaatggagc ggatcgttca
     5521 accagacctg gaacgacgca cgcaagcgcc tgaccttccg cggcgatacg acggtctacg
     5581 ccgggcgctt cctcggtgcg acgcaccagt tcaagttcgg catgtcgatc gagaacgaga
     5641 actacacccg cgatctgtac cagcgcccgg tcgtgttcta ctcgcagctc accttccagg
     5701 acccgacgcg cccgcaggac gacccggtgc ccaagatcct gatcgacggg acgttcgccg
     5761 cgccggatta caccagcatc aacgccaagg gcgtcaactg gggcttttac gccgaggacc
     5821 agttcaagcc ggcgcagaac ctgacggtca cgctcggcgt gcggatcgac cgcgaggaga
     5881 ttcgctcgaa cggcaaggtg ccgatcgatc cggaagcgga gttccagaac tatctcgggt
     5941 tggtggaagc aggtgacccg gtcaacgccc acgcgcggtc cttcacggcc tacgaagacc
     6001 cgcgggagtt cgtccggacg ctcgcgtctc agctcggcat cgacttcaac acgttgtggg
     6061 gaagcctgtc gaacgcctcg acgcagagcg agttctggcc gacgtcgcgc gccaacggga
     6121 acgtcgacgt ggtcaatacg aatccggcgc cccggctggc gctgtcatgg gatccatgga
     6181 gcaacggcaa gacgaaggtc ttcgccaccg ccggtcgcta ctacgacaag atcttcctcg
     6241 gcgtgccgct gatcgagctc aacccgccga ccaccaacct gaatttcgaa tcgttcatcg
     6301 atcctaataa cgggcggcgc gtcgttcagc tcaacgaggc ggtgaaggcc gcggtcaacg
     6361 tcaataccgt cgaccgcgat ctcgagaccc cctacaacga cgaatggacg ctcggcttcg
     6421 agcgcgagat cgcgaccgag acctcgttgc gcgtgaccta cgtcaaccgc cgattccgca
     6481 atcaactgca ggatctcgac ctgaaccacg cacccggtga ctacggccgc gactgcaacc
     6541 cgcggggcga gccgacgttc gtcgtcccgg tccaaccggc cgatcccggc tacgacccgc
     6601 gatacgcccc cggcgacggt gtctacgacg actgcgtcgg caggatcgag ttcgatcgca
     6661 tccagtcgga agttgttggc ggaaacggcg gggcccaaca ggacgaggcg atcggtacgc
     6721 tcgagttccc cgacggattc gccgatctct acgggctgaa tccgggttgg ggcgacatgt
     6781 acctcgtcgg caacctgaac aggatcgact acgaggggct gacgatcgag ctgatccgcc
     6841 gccagtaccg cagctgggag atgaccgggt cctacacgtt gtctgaggca ttcggcgacg
     6901 gcgaggactt ccagcagaac ctgggtgacg accgcagcac gatcgaagac gagcaaggct
     6961 atcaggccta cgaccgtcgc cacgccgtca aggtcgcggc gacctcgatc acaccgtggg
     7021 gattccgtct cggcgccaat ctcacctggc agtcggggct gccctactcg ctgctgacgc
     7081 ggcaggtgtc gttcgacgcc gcggcgccgc cgcacgggac gttggctccc gtcggtgttc
     7141 ggcgccagcg cacgacctac gataccggcc agcgcaacga ccagcgcaac gactcgttct
     7201 ggaacctcga cctcaagctg acgaaggaga tgaacgtcgg ccgcaagctg aacatgcagc
     7261 tgtcggccga aatcttcaac gcgctcgccg acgacaccta cacgatctac gacccgatca
     7321 accaagtcgg cgagcaggtc aacggcgtca ataccgcctt ccgtcggttc ggacggcgct
     7381 ggcagctcgg tgtgcgtttc tcgttctagt tcgatcgtcg ggaaccacgc atcgctcgag
     7441 aaggagccgc ccgacgggcg gctccttcgt cttgagacga ggccgttcgt cgcgctgtga
     7501 agttgcagca cgatcggcct aggccttgct ccggcagccg gtctccgggt aagagtgggg
     7561 ggtagagggg tcgagcagct ggaggggggg gtgggcgaac gaacggccgt catcgtcctg
     7621 tttgccgccg cgctgcttct caccgcatca ccggcgcgcg cccaatccgg tgcgatcgcg
     7681 gtcgaggtgc tcgacgccga ggggcggccg ctccccggtg tcacggtcga gatcggccac
     7741 ccgacgggct acgtcaaacg gaccgccgtg ctgaccgacg ccgacggcca cgccgacttt
     7801 ccggtgctgc gtgccaccgg cgccgtcggt ccgggctacg agctgacgat ccgtctggcc
     7861 gggttctcga ccgtccgcct gcccgacgtc aaggtccgcg tcggcgagac caatcgcttc
     7921 cccgtccgct tgacgaccca gctcgaggaa cgggtccgcg tcgagggcgg ctcggaggtc
     7981 gtcgacctcg aggacacgac ccagacgacg aagttcagtg acgagttcat gcaggatctc
     8041 ccggttgccg gtcggttcta ccagaacgta ctgaccttgg cccccggcgt gcaggatgcc
     8101 gacggcgacg gcaacccgaa cgtccacggc tcgcgcgatc gcgatttcaa ggccgaggtc
     8161 agcggtgtgt cgaacgtcga cccgttgacc ggccagcaga tgagtcagat caaccccaac
     8221 tcgatcgagg agatggaggt gatcaccgcc ggagcgggtg tcgagttctc gcgcgcgcag
     8281 ggcgggtttg cgcggatcct gcagaagcaa ggcagcaacg acctcgaggg cgtcgtcgag
     8341 ttctacatcc gctcttcggt cctcgacggg accggcgcgg acgattccaa caacacgacg
     8401 cagcccgatt tcgcctcgta ccagcccgcg ttccaacttt ccggaccgat cgtccgcgat
     8461 cgcctgtggt accggttgtc gcacgagtgg atctacaccg aagacccgca gaacacgacc
     8521 cgcggcatct cgatcgtcga ggatcgctgg gcgatccact cggaccagct cacctggcag
     8581 gtatccccgc gaaacaagct cgcgtttcag taccaggccg acccgcgcac gatcgacaac
     8641 ttcggcgtca actcgacgcg acccgaggag gcggcactgt tcatcgagaa cgagagcgaa
     8701 agctatgcgc tgacgtggac cgcgccgctc tcgacgcgga tcctgatcga cagccgcgtg
     8761 gcctggcagg atttcaacgt caccgtcgcg ccgaccgagg tcggcgtgga gaaccggtgt
     8821 ctgaccggcc ccgcgtttct cgagcgtgcg cagtgcttca atctgttgac cggcgagcgg
     8881 agcggatcgt acccgcagga ctggaacgac catcgccagc ggctgacggt gcgcggagac
     8941 gcgacgatct acggcgggcg tttcctcggg gcggaccatc aactcaagat cggcgcgatc
     9001 gtcgagaacg agcgctacac gcgcgagctc gaacggcgtc cgaccgtttc gttcaccgtg
     9061 atcaacagta ccgaccagac caacaccgac gaagactcgc cggagcgggt cgcggtcatc
     9121 tcgggcagct tttcggtccc ggagttcacc gaagtgacgg cgcgcggcgc gacctggggg
     9181 ctgtatgccg aggaccggtt tcggccggcg accaacttga cgctgacgct cggggcgcgc
     9241 gtcgaccgcg aggagatccg ctcggcgggt cgcgaaccgc tcgatccgca gggtgaagcc
     9301 gccgagtacc tgagcctggt cgagagcgga acctccgcgc agatggcggc gccggccgtg
     9361 ttcaccgcct acgaagaccc gcgggacttc ttccggcagc tggccgcgat gctcgagatc
     9421 gatttcaacg agttgttctt gagtcagtcg ccgatggcgg tccagagcga gttctggcag
     9481 aactcacgcc atcccggctc gatcgacctg gccaatacca acttcgcgcc gcgtttctcg
     9541 ctggcgtggg acccgggtcg ggacggcaag accaagctcg ccgttaccgc cggtcggtac
     9601 tacgacaagc tcttcctcaa catcccgctg atcgagttgg agccggccag caccgacctc
     9661 gtcttcgagg cgtttccgcg gggcctggac tggcaggtga ccgggctgcg tgaagcggtc
     9721 aacccggcgc tcaacgtcga cgccgtcgat cgcacgctcg acaccccgta ccaggacgaa
     9781 tggaccgtca gcttcgagcg ggagctggcg ctcgagacgt cggcccgcgt gacctacgtc
     9841 aaccggcgtt accgtgatca gctccaggac atcgacatca accacgagcc ggacgactac
     9901 ggccgctgcg tcacggcgtc accgagcaat ccgcggttga cggtgacgcc gattctcccc
     9961 agcgacccga cgtacgaccc ggcggtcgca ccgggcgacg gcgtcgtcga cgattgcatc
    10021 ggccggctgg tcgtgatcaa cgatccggag tcgccgggag gttcggtgct gctcccgcgc
    10081 cccgatggca ccgccgatct gtacgtgcag aacccgggct ggggcgacgt actgctggtc
    10141 ggcaaccaga atcggatcga ctacgaagcg gtggtgttcg agctgactcg gcgccagtac
    10201 cgcaactggg aaatgcaggg ctcctacacc tggtcggcgg cgcgcggcga cggcgaggat
    10261 ttctcgcaat cgctgggcga cgatcgcagc ctggtcagcg acgagcaggg gttccaggcc
    10321 tacgaccagc gacacgtggt caagttcaac gccaccacga tcacaccgtg gggtttccgg
    10381 ctcggcggcg cgatcagctg gcagtccggc ctgccctact cgctgctgac gcggcgtcag
    10441 gcgttcgacg cggtcccacc gcagtacggc accctcggct ccgccgcggt caggccgcgc
    10501 acgacctacc cgtcgggcac gcgcaacgac caacggaacg attcgtactg gaacgtcgac
    10561 gtcaagctga ccaaggagct gcgcgtcggc cgcggcgtcg gcctgcagct gtccgccgag
    10621 gtgttcaacc tgctcaacga cggaacctac ctcatctacg atccggtctc ccagaccggc
    10681 gagcagatca acggcaacaa cgtcgcgatc aggcgtttcg gccgtcgctg gcagctgggc
    10741 gcacgcgtcg ccttctgatc gcgcgagcca ccgcgggcct gctagaatcc ccgcggtccg
    10801 tccgacgaga tttccccgga aagacgatga agatctacaa cacgctgacc cgcaggctcg
    10861 aggggttcga gccgctcgtt ccgggcaagg tgtcgatgta cacctgcggg ccgacggtct
    10921 acgacttcgc gcacatcggc aacttccgcg cctacgtttg ggaggacctg ctgcgccgca
    10981 cgttcgagca cctcggattc gaggtgacgc aggtgatgaa catcaccgac atcgaggaca
    11041 agatcatcgg caagatggcc gagctggatc tcgacctcga ttccgtcgtc cagccgttcg
    11101 tcgatgcgtt cttcgaggac ctggccacgc tgcgcatcag gcgcgccgag cactaccctc
    11161 gggcgaccga gcacatcgcc gaaatgacac agctcgcgct cgagctgcgc gatcgcggtc
    11221 tgacctacga gagccagggg tcgctgtatt tcaagatcgc ggcgttcgcc gggtacggcc
    11281 gattgtcgaa cctggacaac cgccggatca agcccggagc gcgcgtcgat tccgacgagt
    11341 acgacaagga agacgtgcgt gatttcgtgt tgtggaaggg cgggaagccg ggcgagccga
    11401 cgtgggactc tccgttcgga cccggccgac cgggctggca cctcgaatgc tcggcgatga
    11461 gcatgaaata cctgggccag accttcgatc tccacaccgg cggcgtggac aacatcttcc
    11521 cgcaccacga gaacgagatc gcccagagcg agggcgccac cggccggccg ttcgtccggt
    11581 actggatgca cgtcgcgcac ctgatggtgg acggccagaa gatgtcgaag tcgaagggga
    11641 acttctacac gctgcgcgac ctgctcgcac gcgggttcga tccacgtgcg atccggctgc
    11701 tgctgctcgc gacgcactac cggacgccgc tcaacttcac gctcgacggt ttgagccagt
    11761 cgacggccga ggtcgggcgg ctcgagagtc tggtcggccg cctggaccgt gagccggaag
    11821 gcggcggccg caacccgagc ttcgacgagg cgatccggcg cgccgagcgc gtcttcggtg
    11881 aatcgctgac cgacgacctc aacatcagcg gggcgctggg ggcggtgttc ggcttggtgc
    11941 gggaagcgaa cgtggcgctc gaccgaggcg agctaccgcg cacgagccgg gcgggcctgc
    12001 gggtggcgct cgagcgcttc gacggggtac tggacgtttt ccggccggcg gccaccggct
    12061 acgcggtcga cgtcgacgac tacatcaagc ggcgcgacga ggcgcgaaag gcacggaact
    12121 gggccgaagc cgaccggatc cgcgaagagc tgtccgctgc cggcatcgtg ctcgaggata
    12181 cgccgcacgg cacgatctgg cggcgcggtc tcaagtcccg gtgaagcgga agggatcccg
    12241 cccggtttcg ccgacggaac gcgtggcgcg acccgcgcgc tggatccccc ggccccgacc
    12301 ggtcagactt ctttcggtgc gggacgggag gtgcctggcg tgggcccgcg acggtcgcga
    12361 cgcgaatcga gctccgacga cccccggcaa cggctgggct cggacggcga acgcgccgcc
    12421 gagcgggtgc tgcgcgaggc ggggctgtcg atcgtcgagc ggcgcttccg gctgaagatc
    12481 ggtgagatcg acctgatcgc cagcgacggg ccgctcgtgg tcttcgtcga ggtcaagacc
    12541 cggcgcggtt cgagccacgg tctcccggcc caagccgtga cgccgcgcaa acgcgcgacg
    12601 atcgcacggg tcgccgccgc ctatctcgtt cgacgcggct tgtcggaacg gccgtgccga
    12661 ttcgacgtcg tgcaagtctt cgccggccgt cgcggcgtcg aacgggtcga ccacatccgg
    12721 gacgcgtttc gcttgtggga cgatgaccga tcgacccgct tcacgcggcg tctcggttga
    12781 cggaacaggg gggctgcggt attttgcgat ggttgtggta ccgggccctc ccgggcgctg
    12841 cccatacggg ttttcgggtt cgatcgcggg aatagctcag ctggtagagc acgaccttgc
    12901 caaggtcggg gtcgcgggtt cgaatcccgt ttcccgctcc acgggtccgc gactggaccg
    12961 gtgcccgagg tggacgatgc gaacacgctc gtggctcgtc ttcgcgctgt cgatcggggt
    13021 cgccggcttg gtgtatgccg cgcgaccgat ggcgctgacc aacggagatc cggcgccgcc
    13081 gatgcgcggc aacctcccca acggtgacct gctgatcgtc gactactcgc cgaccgagct
    13141 gacgctgatc aacttctggg ccacctggtg cgagccgtgt cggaaggaga tgcccgcgct
    13201 cgacgacctc taccggcgcc acgcgggcgc gggcctgcag gtgatcggcg tgcacgtcgg
    13261 gccgctgacg gacgacgagt acgcgcagtt caaagaggcc gtgccggtcg actatccgct
    13321 gatcctcaac ccgagcgcgc gctggctgag cccgtggggc ggcctgtcga tcctgccgcg
    13381 cagctacctg gtcgccagcg acggccgcat cctccgtggc tacgtcggcg cctcggccga
    13441 acaggtcgaa gcgctggtct acgacgtcga ggcggtgctc gacggccggc cgctcggtcc
    13501 ggtggtggtt ccgacggagc cgagcgacgt gtcgaccccc gaagaccggg atcgagccaa
    13561 ggacgagacc gaatagcgaa gggtcgctca gccgaccacc cgcagccggt gcgatcccgc
    13621 atcgggcgcc gggtccgggc gatggcgctc cgcgtggtac gaggaacgga ccaacgggcc
    13681 ggactcgacg tggagaaagc cgagctcggc gccgatcccg cgaaaacggt cgaactcggc
    13741 cgggctgacg aagcgcgcca ccggcaggtg ctccttggtc ggctgcaggt actgccccag
    13801 cgtgacgacg tcgacgcccg cctgcttcag ctcgcgcata accgcgacga cctcgtcgtc
    13861 cgcttcgccc agaccgagca tcaggctgga cttgacgcga agcgccggct gcatccgctc
    13921 gcgccaggcg gccgcgctcg ccaacagctc gaccgagccg cgataacgcg atcccgggcg
    13981 agccgtgcgg tacagccgcg gcacggtctc gacattgtgg gcgaagacct caggcgcggc
    14041 gcgcagcacg agctcgggtg ctccgcgctt gttcttgaag tcgggcgtca gcacctcgac
    14101 cgcgcaggag ggcgagcgcc ggcggatcgc cgcgatgacg tcggcgaagt gccgcgcgcc
    14161 gccgtcgtcg aggtcgtcgc ggtcgacgct cgtgatcacc gcgtgacgca gcctcaaccg
    14221 ggacacggcc tcggccacgt tccgcggctc gtcggggtcg acgcccggcc gcggcagacc
    14281 gctggtcacg ctgcagaacc cgcagcgccg cgtgcaggtg tcgcccagga tcatgaacgt
    14341 cgcggttccc gcgttccagc actcgtagat gttcgggcaa cgcgcttccg tgcagaccgt
    14401 gttcagccgc agctcgcgca ccatcgagcg gaggccgcga aagccgtcat cggttcgcaa
    14461 gcggatcttg agccaatccg gacggggtcg attgccgcgc tcgtttgcca tggtcaagag
    14521 catacctcgc cgtccagcgg cttgcccagc cgctcatcgg gccgcggcga ggcgaccggc
    14581 cggcggccga agacgcgaca gaacgagcga acgacccgcc acgccacctc cggcatcgcc
    14641 ggctcgacgc cgcagcactc ggcgagcgag gtgacgccgc gaccgtggag cccgcacgga
    14701 acgatcgcgc gaaacccgtc gaggtcggta ccgacgttca acgcgaagcc gtgcgaggtg
    14761 atccatccgg tcgacaaccg gacgccgatc gcggccagct tgcgctcgcc gacccatact
    14821 ccggtcaggc cctcgacgcg ccggccggtg acgccgagct cggcgacggc atcgatcagc
    14881 acctgctcga gctcgcgcag gtaacggtgc gcatcgcgcc gctcgggcgc gagagcgagc
    14941 acggggtagc cgacgagctg acccgggccg tgataggtca cgtcgccgcc gcgtccgctc
    15001 tcgtggactt cgatcccgcg tcgcgcgagc gtctcccgat cgacgagcag gtgagccatg
    15061 tccgcgccgc ggccgcaggt gatcaccggc tcgtgctcga gcagcagcag cgtgtcgggg
    15121 acctcgtcat gccgccgtcg cgccaccagc cggtcctgca gcaccacgcc ggcggcgtat
    15181 ccgacctggc ccagccaacg aacgagcagc ggctgcacgc gcggtccccg gcctagagca
    15241 gcgactcgtc cgcgctctcg agcccgcgtt tgaccatcgc gaggaactgg tcggcgaccg
    15301 cgccgtcgac gacgcgatgg tcgtacgaca tcgacaggta gcacatcgat cgtatcgcga
    15361 tcgcctcgtc gatcacgacg gggcgctttt cgatcccgcc gactcccatg atcgccacct
    15421 gcggttggtt gatgatcggc aggccgtaca acccgccgaa ctggccggga ttggtgatgg
    15481 tgaacgtccc gccctgcacc tcgtccgggt tgagcttctt ggtgcgggcg cgatcggcca
    15541 ggtcttgcag gcgcttggcc agaccggcga tcgacagctc gttggcgtta cgcagcaccg
    15601 ggacgatcaa gccccagtcc agcgcgaccg cgacgccgat gttcagttcg cggtggtaca
    15661 cgatctcgtc gccgtcgacc gaggcgttga cgatcggcca ggcctcgagc gcgtcgcagg
    15721 cggccttgat gaagaacggc gtgaccgtca gcttgacgcc gtggcgctcg acgaacgact
    15781 gtcgatgccg ggcgcggagc tgcatcgccg ccgtcatgtc gacctcgaag acggagtgca
    15841 cgtgggccga cgtgcgccgc gactcgatca tgtgttcggc gatcttcttg cgcatcaccg
    15901 acatccgctc gcgcacgacc gagcgcgagc cgaactcggg tgtcgccgct cgcggcgcga
    15961 ccgccgcggg cgctgccgcg gcgggtcgag cggtcaggga gggcggcgcg tccgccgcac
    16021 ccgagcggga ttcgaggaac gccagcatgt ctttcttggt cacccgaccg ccggcaccgc
    16081 tgccgcgcac ccgccccgga tcgacgccgt gctcggccgc gagcttgcgc acgacggggc
    16141 tggcgaagcg cgagccagcg gccgcggccg tttccctgcc ggccgccggc gccgccgcgg
    16201 acgatgcctg cgccgcgccg gtccgtcccc cggccgcgtc gtcgccgctc ggttcgccgt
    16261 tggtttcgat caccgcgacc acgtcgttga ccggcacggt gtcgccttcc gcgctgcgga
    16321 tctcgaccag caccccggcc gagggcgccg ggatctcggc atcgaccttg tcggtcgaga
    16381 tctcgaagat cggctcgtcc cgctcgacct tctcgccgac ccgcttgatc caacgcgtga
    16441 tcgtgccctc ggcgatcgac tcgcccatct gcggcatcgt gacgtcaaca cgcatctctc
    16501 gtctccatcg ggcacccggt gccccggcgg accgggttcc gccccgtcag aggtgaatcg
    16561 ctcggtcgtg gaccgccagc gcggcctctc ccatcgcttc ggacaacgtc gggtgcgcgt
    16621 gcacggcgtg gaacagcgac tcggcggtgg cctcgaggct gaccgcggtc gtcgcctcgg
    16681 cgatcaactc ggtcgcgtgg ggtccgatga tgtggacccc gaccacctcg tcgtagtcct
    16741 tgtcggtcac gatcttgacg aacccggtgg tgtcgccgag gatcttggcc ttgccgatcg
    16801 cggtaaacgg gaacttgccg accccgacgt tgatcccgcg ctcgaccgcc tgccgctcgc
    16861 tgagcccgac cgacgcgacc tccggatgac agtaggtgca cgacgggatg cggtcgtagt
    16921 cgatcggccg cgggtcggcg cccgccgcgt gttcgaccgc aacgataccc tcgtgcgagg
    16981 cgacgtgggc caacgcctgc gtcgcgagca ggtcgccgat cgcgtagacg ccggccatcg
    17041 ccgttcgcat catctcgtcg acctcgatga agccgcgcgc gtcgagctgg atcggcgtcc
    17101 cgtcgagccc caaaccctcg gtccgcggcc tgcgtccgat ggcgacgagc agcacttcgg
    17161 cgtcgagctc gagtcgtttc ccgtcggcct cggcgacgag cttgaccccc tgcgggtccg
    17221 tctcgactgc ggtcacccgc gtcgaggtgt gcgaggcgat cttgcgcttg cggaacgcgc
    17281 gctcgagctc cttgcccagc tcttcgtcct cgagcggaac gagccgcggc agcatctcga
    17341 cgatcgtcac ctccgagccg aacgagcggt aggccgaagc gagctcgacg ccgacggcgc
    17401 cggcgccgag cacgatcatc gacttcggga tgcgctcgag cgcgagcgcg tcatccgagt
    17461 tgatcacgcg cttgccgtcg aactcggttc cgggcagctc acggatgacc gagccggtcg
    17521 ccagcaccac gtgcccgctc tcgatctcgt acggactgtc ctgcggcggg gagacgcgca
    17581 cccgtccctg gcccgccagg ctgccgacgc cacgcagcag cgtgacggag ttcttcttga
    17641 acaggtactc gacgccgcgc acgttggcgt cgatcgtctt cttgcggtac gcatgcatcg
    17701 cggagacgtc gagctcggcc ttgccgatct tcacgccgaa cgaatccgct ctccgcgccg
    17761 tgtcgagcac ctcggctgtg cgtagcagcg ccttggccgg gatgcagccc aagtgcagac
    17821 aggtcccgcc cggtgcggcg tccttctcga cgaccgcggt gcgcataccg agttgcgcgg
    17881 cgcgcaccgc ggcgacgtag ccgcccgggc cgctgccgat gatcgtgatg tcataccggc
    17941 cttcccgtct cgcgctcacg acccggactc cttcgacccg ccaccatggc cggttcggcg
    18001 ctcgatggcc acgcaggctg cgggcttgcg aatcacggct ggttgaataa gttgcgacgt
    18061 ttgcgggaga tgcactctaa ccgagcgccc cgacagcgtc aagcaaaccg gcctttcgcc
    18121 gcggcaaccc gctcggtccg ccggccgggc gcccggcgcg cggccgctgc taagctcgac
    18181 gcccccgatc gcgcttcccc ggacgaccga ccatgcccga tgcgccccgc tggtacaccc
    18241 cgagaaccgc ccgccgcgtg ctgcgccgga tccgaccgac ggtcgagctc ctgcaccggc
    18301 tctgggtcga gctcgactac cgcagaccgg atcccatcgg ctccgatctc gccgtcgatc
    18361 cgttgtactt cgtcatggcc cggcgtctcg cgcacgggct ggcggctctc gaggctcgcg
    18421 gtctgctcgt cgacgagctg cgagacggca ggctcgactt ccccgcatgg ctcgacgacc
    18481 gcccggtggt tctgtgctgg cgcgtcggcg agcccgagct gcgcttctgg cacgttcgcg
    18541 gcaacggtcg tccacgcgtg ccgtttcgcg acggcgaccg gttccgcccg cccggcgcgc
    18601 cccgacggcg accgtattcc ctgtgctaga ctcgaattcg acagccctgc ggaggtccgc
    18661 acgtgtcccg acccgacccg aagctcgtcc tgcaccagcg cccggacggc gagcaggaga
    18721 tccggcaaac cgcctacacc acgcgcatcc aggacttcat caactggggg cgcaagaact
    18781 cgctgtggcc gatgccgatg ggactggcct gctgcgcgat cgagttgatg gccaccgtcg
    18841 gtccgcgata cgacctggcg cgcttcggcg cggaggcgct gcgcttttcc ccgcgccagg
    18901 ccgatctgat ggtcgtctcc ggcacggtca ccaagaagat ggcgccggcc gtgcgcaaga
    18961 tctacgacca gatgccgaac ccgcgatggg tgatctcgat gggtgcctgc gcctcgtccg
    19021 gcgggatgta ccgctcgtac tcggtggtcc agggcgtcga cgagatcctg cccgtcgacg
    19081 tctacgtcag cggctgcccg ccgcgtcccg aggcgttgct cgaagcggtg atggagatcc
    19141 agcgcaagct cacccgtcgc gagaagagcg cgtccgagct ggccggcgcg cgaccgctcc
    19201 cggtctgacc gccgcggtcc gcccggtcgt gacccgcgaa cagccgaccg gcgacccccg
    19261 gctccacgca ctcgttctcg acgagatccg ttcgacgggt ccgatgagct tcgcccgctt
    19321 catggagctc gcgctgtacc acccgaccca cggttactac gcggcgggag catcacggct
    19381 cgggcccggc ggcgacttca tcaccgccag cgacgtcggt cgcgccttcg gccgcgcgat
    19441 ggcccggcag atcgaccaga tcgaccgggt ggtcggtccg ttccatccgt tctcgatcgt
    19501 cgagtacggt gccggtcgcg gcatgctcgc cgccgacgtg ctcgacgccc tgcgtgacga
    19561 ggcgccgggg ttgtacgagc gcaccgagta cgtcctggtc gaccgcagtg agaagatgcg
    19621 cgaggcggcc agggttcgcg ttccccgggc gcaggtcgtc gcacccgagg tcgcaggtgg
    19681 cgggcgccgc ggagtggtga tcgccgtcga gctgttcgac gcgctgccgg tgcaccgggt
    19741 gcgccgccgc ggcgggcggt tggtcgagct acgggtcgac gccgatccgg acggcctgtt
    19801 cgtcgagcgc gagaccccgg ccgacgagac cgtacgtgac tgggccgcgc gctttggcgc
    19861 ggcgaacgac gaggggtcgg aagccgaggt ctcgctgtcg gccgagccga ccttcgaccg
    19921 gatggcgtcc gccgtcgagc gcggcgcgat gatcgtcgtc gactacggcg atcgctcgcc
    19981 cgcgctctac ggcgacacgc gacggcgcgg cacgctcctc gcttatcacc gacacgcgac
    20041 ccacgaggac tgtctggccc gggtcggcag ccaggatctc accgcccacg tgaacttcag
    20101 cgtgctcgaa gagcgcgccg tcgagctcgg ctgggacgtg ctggcgctga cgacgcagga
    20161 tcgcttcctc gtggccaacg gcatcctcga ggagttcgaa cagcgcgacc tggccgagtc
    20221 gcacgacccg gcgcgcgtca agcgccgcct gcaggcgttg cagctgatcc accccgaggg
    20281 aatgggacgg atcttccgtt tcctgctgct gtcgaaaggc gcgcaccccc cgccggcgct
    20341 ggccggcgcc gccgacccgt tcggccgcgg gcgctgatcg cggcgctata ctcggacgat
    20401 ggtgcgcgac cagtctccga tcgatccgca cgcgatcgag caagccttcc tcgaccaggg
    20461 cttcgtctgc acgggcgagg tggcgaccac gctgtacctc gccgagcgtt tggcgaagcc
    20521 ggtgttgctc gaagggccgc cgggcgtcgg caagaccgag atcggcaaac tgtgggcggc
    20581 gtaccacgcg gcccgcctga tccgtctcca gtgctacgaa gggctcgacg agtcgaaagc
    20641 gttgtacgag tgggcctacg gcaagcagat gctctacacg cagctgttgc gcgagaagac
    20701 cggcgagctg ctggccggcg cgaccgacct gcgcgatgcg atcaaacggc tggccagcca
    20761 gaccgacgcc ttcttctcgc gcgagttcct gctcgagcgg ccgttgctcg ctgcgatcgc
    20821 ctcgcccgac ccggtcgtcc tgctgatcga cgagatcgac cgcgccgacg aggagttcga
    20881 ggcgttcctg ctcgaggtgc tgtccgatta ccagatctcg atccccgagc tcggcacgat
    20941 tcgggcgcgg cgtccgccgc gcgtcatcct caccagcaac gattctcgcg acttggccga
    21001 cgccctgcgc cggcgctgcc tctacctgta cgtcgactac ccgggtcacg acgacgagct
    21061 gcggatcatc gaacgccgcg cacccgcgct gggtcagacg ctcgcgcaaa acgcggtgcg
    21121 gttcgcccag tcgctgcgca aggccgagct gcgcaaacag cccggagtcg ccgaggtcat
    21181 cgactgggcg ctcgcgctgg tcgcactcgg cgcgcgcgat ctgtcgccgc gtatcttgcg
    21241 cgagacgctg ggcgcgctgt tgaagaaccg cgacgacctc gagcgggtgc tgtccgaggt
    21301 cgggcggacg cgccggtgat cgaggcgctg caacggttct tcgaggtgct gcacgacggc
    21361 ggcgtccgga gctcgccggc cgagcggctg gacgcgctgc gcgcgctcga gtgcgtgggc
    21421 gtcgcagacc gtgcgcgggt gaagcaggcg ctgcgcgcga ccttggtcaa gcacgcggcc
    21481 cagcggggcc tgttcgacac gacgttcgac cggttcttcg ctgcacccgg gcgtgcccgc
    21541 cgcggtcggc gacgacgagc gggagcgaag ggcggcgagg tgaagcggac ggccgatgcc
    21601 tcggccgccg ccgccggagc gccgcgcggt cgggagccgc gccaccccga gcgcgatcgg
    21661 cggtcgcccg aggcgctgcg acgcgccctg gccgagctga gggacgggcg ccgcgaacgc
    21721 tggggacggc tgcgacaggt cgcggttcga gcgccacgcg agcgcgagcc cgacgcgtcg
    21781 cgcccgcgtg ggcgaacggc gctcgtttcg ctgcgtgccc ggccggaggc gaaccggcgc
    21841 aacgagatcg agcgcgagct gcggcgactg atcgagcgga tccggatggg cgccgcgcgg
    21901 cgcctcgagc gcgcctcgcg gggccgcccc ttcccgcggc ggctgttccg cgacagcctg
    21961 gccagcgacg gggtgccatt ccgcatcccg cgccgccgcc gccgcacgcg cacgccgcgg
    22021 gtctggctgt tgatcgacgt gtcgtggtcg acggcccacg cggccggctt gttcctcgag
    22081 atggcgagcg agttcgtccg cctcggtcgc cagtgccgcg tcatcttctt cgtcgatcgt
    22141 ccggtcgacg cgacggaaca tgtcgaggat tggatcgcgc ggggcgatct cgcggccttc
    22201 gtcgatcgcc tgcgcacggt gcccggcgtg aaccccgacg cgccgagcga ttacgggcgc
    22261 accttccacc ggctgctcgt ttctccgcgc cgcccgcgtg ggcggcggtc gctgctcgtc
    22321 gtgctgggcg atggacgcaa caacgagttc gacccgcagg cctgggcctt cgccgagttg
    22381 gccgatggct gcggggcgac gctgtggctc gtgccggagc cgatcgaggc ctggggcggc
    22441 ggcgattcgg cgctcgccga atacctgtcg agtgtcgaca ccgtggtcga agccgacgac
    22501 ctcgacgggc tcgcgcgggg tgtggcacgg ctggtgaggc ggctgtgatt ccgttccccg
    22561 gggcgctcgt ggcggtgtgg gaggcgggag agctgacgct cggggtggtc gccggcgagg
    22621 agaagcgccg gttgcggctg gtgctcgagg gcggtcgcga gattcgggtg cagccggccc
    22681 ggatcgcgtg ggaggtcgag gcgccgggca aggtaccggg ggcagcgccc gaggagcggc
    22741 gcgaggcagg cgagcgcgtc gagcgggccg aacggaggct gcgcggtgtc gccgactcga
    22801 tcgacgtcga gttggtttgg cagatcctgg ccgaggccgg cgcgccgccg gcggacggct
    22861 tcgagaccgg cgacctggcc gagctcgcgc tcgactcgcg ctcgggagaa tcgatggcgg
    22921 cgacggtgcg cgcactgcgg cgggacggtc tgcgcttcac gcgcaagggt gaacgttggc
    22981 tggcgcgagc ggccgagcag gtcgccgacc tcgagcgcga gcgcgagcgg atcgccgagc
    23041 gcgagcttca gacgagggcg ttgttcgacg cgctcgccgc ggccgtgcgc ggccaggcat
    23101 tccacgagac ggggagcgag tacgaacggc ggtacctcga cgcgctgacc cggctggcgg
    23161 tcgacgcgga gtcggcgccg gaggtggccc gcgcgctggc ggtcgaggcg ttgtccgcct
    23221 ccgggcttcg ctgcgagcgg cctcacgagg gggcgttccg cctgctgcgg ctcgtcggcc
    23281 gtctcgagca cgacgacgtc aacct
//
LOCUS       DASPGN010000474         6181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_323351, whole genome shotgun sequence.
ACCESSION   DASPGN010000474 DASPGN010000000
VERSION     DASPGN010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323351"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VD788_13840"
     CDS             <1..531
                     /locus_tag="VD788_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77394.1"
                     /translation="KVTEATPTHLFVLGPDEAHAAIVEMLAERDPAVPAAQGFEVTLV
                     RAVRPPAGDPPGSFPPRIATALRGLLELMPDLRFEPMATGSVRTSDRGRIRLATENGE
                     RYVVEIEYRSRRVGLERTELSLAIEAERDSNNPNLRDLLATSLSVFVGETVVVGSSTT
                     SNSQDPMVLLLLTVVD"
     gene            652..2484
                     /locus_tag="VD788_13845"
     CDS             652..2484
                     /locus_tag="VD788_13845"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HXV77395.1"
                     /translation="MTSVLGISAFYHDSAAALLVDGDVVAAAQEERFTRRKHDHRFPQ
                     HAIDYCLAEAGLRPEQLDYVAFYDKPLLKFERLLETYLAFAPAGFRSFYLAMPLWVRQ
                     KLHLPRLIRRGLAGGYRRGIVFPEHHESHAASAFFPSPFDEAAILTFDGVGEWATASY
                     GVGRGNWIELTHELRFPHSLGLLYSAFTYYCGFKVNSGEYKLMGLAPYGEPVYAERML
                     ESVLDLKDDGSIHLDLSYFNYCHGLTMTSSKLNRLFDGPPRRPEAPLTERHMDIAASI
                     QQVTEEVVMRAARHVHAETGMRNLCLAGGVALNCVANGRLMREGPYERIWIQPAAGDA
                     GGALGAAQLVWYQLLDRPRTPQEGDAQHGSFLGPRFESAEIRRFLDDRGSVYRHFDDR
                     GALIAEVAELLANEKVVGWMQGRMEFGPRALGGRSILGDPRSARMRSLMNLKIKFRES
                     FRPFAPSVLRDRVDEYFETRPREDSPYMLLVAGVQPNKRLPPDGVRLRGLDRLKAKRS
                     LIPAVTHVDYSARIQTVDERRNPRYHELLRVFESKTGCPVLINTSFNVRGEPIVCTPE
                     QAYRCFMATEMDALVLEDVLLLKAEQPEAAGDDRERYFAVFEPD"
     gene            2493..2909
                     /locus_tag="VD788_13850"
     CDS             2493..2909
                     /locus_tag="VD788_13850"
                     /inference="COORDINATES: protein motif:HMM:NF039992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HXV77396.1"
                     /translation="MALIDVDWNPRARDLRVFALLFLVFAVGAGTVLYFRGDPRALSL
                     ALWIAGPVVAVLGLLLPRALRPLYVVMMAIALPIGMVVSTALLIMIYYLMITPIGWAM
                     RLSGHDPMGRRVDPAAESYWVRRRVVDDPKRYFRQY"
     gene            2924..3160
                     /locus_tag="VD788_13855"
     CDS             2924..3160
                     /locus_tag="VD788_13855"
                     /inference="COORDINATES: protein motif:HMM:NF039953.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HXV77397.1"
                     /translation="MSGPSSDERTTSGHGGRDGSEFAREAESSQPGILREFGEFLLHN
                     KKWWLAPIVIILLLFGLLIVFGGTSAAPFIYTLF"
     gene            3192..5426
                     /locus_tag="VD788_13860"
     CDS             3192..5426
                     /locus_tag="VD788_13860"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV77398.1"
                     /translation="MESQTGKTLSHYKILRRLGAGGMGVVYEAEDGRLGRRVAVKFLS
                     EELQNDLPALERFQREARAASALNHPGICTVFAIEQFERQHFIVMELLEGQTLAQRIG
                     QQPFEIGALLDVGIQVADALESAHSKGIVHRDLKPVNLIVNERGQVKILDFGLAKVEG
                     APAAIASDASSSQVETAVGRGQLTAVGTVLGTMFYMSPEQARGQLTDARTDLFSLGVV
                     LYQMAAGELPFQGDTQAVVFDAILNRDPRPLAEVNPAMPQALGQILDKALERDRNLRY
                     QTATELKTDLLRLRRKLDSGRRHAAEAAEAKSAPGKRAKRSIAVLYFENLSGVKDDEY
                     LRDGITEDIITELSKIEGLKINPRSTVLAYRDKPVTSAQVGQQLGSAYALEGSLRRAG
                     DRLRITTQLVDTETGYPVWSERYDREMKDVFEVQDEIACKIAEALRIKLSPKEQEAIA
                     AKPTDNLQAYDLYLRGRSYARRLTRQDLEFALQMYENAVTQDPHFALAYAAIANVCAY
                     YHAHYAREDVWIHRARAAAERAMALQPDVPEVMVAQAWILYAKAQYDEMTAIVRNVIA
                     RKRECEGAYYLLLRGLYASGKYQDVANVADEALESSGTDYNVYVPIMNSLGALGKDEA
                     LRNIRQRAIQAFETHLRQVPEDARARILVGVNYAAEGRHDDAVRETNLAMLLRPNEAM
                     VHYNAACTFCLMERKGEAIDALAKAWRNGFKDADWARRDPELAILHGDPEFERLYPVS
                     PSDA"
     gene            complement(5460..>6181)
                     /locus_tag="VD788_13865"
     CDS             complement(5460..>6181)
                     /locus_tag="VD788_13865"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77399.1"
                     /translation="ATNLDLLGRPDEARARLRQLYDQALNDGQRRTALGAMAVSYIAG
                     GDFPGALTQVQRQYDLAAAAGDHAAMSADVNFMGNILLEMGRFDEARARFEETVALLD
                     EAQNTSEEVKDLARLTYHYDAGRVAAWSGQLPEAKEHLSEYQSRAEANRNQFQIWAAH
                     QLAGIIAIQEQDWDEAVSRIGRSNPQNPFNSYLLGVAYRGQGDGERAKQQFDRAANFN
                     VAGNAQLAFARQRLGASSDDT"
BASE COUNT          986 a         2123 c         2101 g          971 t
ORIGIN      
        1 aaggtcaccg aggccacccc gacccacctg ttcgtgctgg gtccggacga ggcccacgcg
       61 gcgatcgtcg agatgctggc cgagcgcgac ccggcggtgc ccgccgccca aggcttcgag
      121 gtgacgctcg tgcgcgcggt gcgcccgccg gccggcgatc cgccggggag cttcccgcca
      181 cgcatcgcga cggcgctccg cggcctgctc gagctgatgc ccgacctgcg cttcgagccg
      241 atggcgaccg gctcggtacg gacgtcggac cgggggcgga tccgcctggc gaccgagaac
      301 ggcgagcgct acgtggtcga gatcgagtac cgcagccgcc gcgtcggcct cgagcgcacc
      361 gagctgtcgc tcgcgatcga agccgaacgc gacagcaaca accccaacct gcgcgacctg
      421 ctggccacgt cgctgagcgt cttcgtcggc gagacggtcg tcgtcggctc gtccaccacc
      481 agcaacagcc aggacccgat ggtgctgttg ctgctgaccg tcgtcgactg atccgggtac
      541 actctccgcg gaaagcgccg ccagccgagc gagcgggaga cgcgtgcctc gacatcacgt
      601 tgccgcgagg gccgtggccg agtatcgatc cctgcggggt ggtctcgaag gatgacgtcc
      661 gtcctgggaa tctccgcgtt ctaccacgac tccgccgccg cgctcttggt cgacggcgac
      721 gtggtcgccg ccgcccagga agagcggttc acccgtcgga agcacgacca ccgattcccg
      781 cagcacgcga tcgactactg tctggccgag gccggactgc gccccgagca actcgactac
      841 gtcgcgttct acgacaagcc gctactgaag ttcgagcgac tgctcgagac ctatctggcg
      901 ttcgcgccgg ccggcttccg ctcgttctac ctggcgatgc cgctgtgggt tcggcagaag
      961 ctgcacctgc cgcggctgat ccgccgcggc ctggctgggg gttaccggcg cggaatcgtt
     1021 tttcccgagc accacgagtc ccacgcggcg agcgcgttct tcccctcgcc gttcgacgag
     1081 gcggcgatcc tgaccttcga cggggtcggg gaatgggcga ccgcgagcta cggggtgggt
     1141 cgcgggaatt ggatcgagct gacgcacgag ctgcggtttc ctcactccct cggtctgctc
     1201 tactcggcgt tcacctacta ctgcggcttc aaggtcaact ccggtgagta caagctgatg
     1261 gggctcgctc cctacggcga gccggtctac gccgagcgca tgttggagtc ggtgctcgac
     1321 ctcaaggacg acggctcgat ccacctggat ctgtcctact tcaactactg ccacgggctg
     1381 acgatgacgt cgtccaagct gaaccggctg ttcgacgggc cgccacgccg gcccgaggcg
     1441 ccgctgaccg agcgccacat ggacatcgcc gcctcgatcc aacaggtgac cgaagaggtc
     1501 gtgatgcgcg cggctcgcca cgtgcacgcc gagaccggaa tgcgcaacct gtgcttggcc
     1561 ggcggcgtcg cgctgaactg cgtggccaac ggccggctga tgcgcgaagg accctacgag
     1621 cggatctgga tccaacccgc ggccggcgat gccgggggag ccctcggcgc cgcgcagctc
     1681 gtctggtacc agctgctcga ccgcccgcgc acgccgcagg agggggacgc gcagcacggt
     1741 tccttcctcg gcccgcgctt cgagtcggcg gagatccgcc gctttctcga cgaccgcggt
     1801 tcggtctatc gccacttcga cgaccgcggg gcgctgatcg cggaggttgc cgagctgctg
     1861 gcgaacgaga aggtcgtcgg ctggatgcag ggacggatgg agttcggtcc gcgagcgctc
     1921 ggcggccgca gcatcctcgg cgacccgcgc agcgcgagga tgcggtcgct gatgaacctc
     1981 aagatcaagt tccgcgagtc gttccgcccg ttcgcgccgt cggtgctgcg tgatcgcgtg
     2041 gacgaatact tcgagacccg cccgcgggag gacagcccgt acatgctgct cgtggccggc
     2101 gtgcagccga acaagcggct tccgccggac ggcgttcgac tgcgcgggct cgaccggctg
     2161 aaagccaaac gttcgctgat tcccgccgtg acccacgtcg actattccgc gcggatccag
     2221 accgtcgacg agcggcgcaa cccgcgttac cacgagctgc tgcgcgtgtt cgagagcaag
     2281 accggctgcc cggtgctgat caacaccagc ttcaacgtcc gcggcgagcc gatcgtctgc
     2341 acgccggagc aggcctaccg ctgcttcatg gcgaccgaga tggacgcgct ggtgctggag
     2401 gacgttctgc tgttgaaggc cgagcagccg gaggccgccg gcgacgaccg cgagcgttac
     2461 ttcgcggtgt tcgagccgga ctgaacacgg cgatggcgct gatcgacgtc gactggaatc
     2521 cacgtgcgcg cgacctgcgg gtcttcgcgt tgctgttcct ggtctttgcc gtcggagccg
     2581 ggacggtcct ttacttcagg ggcgatccgc gcgccctgtc cctggcgctg tggatcgccg
     2641 gcccggtcgt tgcggtgctg gggttgctgc tcccgcgggc gctccggccg ctgtatgtcg
     2701 tgatgatggc gatcgccctg ccgatcggca tggtcgtctc gacggcgctg ttgatcatga
     2761 tctactatct gatgatcacg ccgatcggtt gggcgatgcg gctgtcgggt cacgacccga
     2821 tggggcggcg agtcgaccct gcggcggaga gctactgggt ccgccgccgg gtggtggacg
     2881 atccgaagcg gtacttccgt cagtactgag agaggtcgat cgcgtgtccg ggccatcgag
     2941 cgacgaacga acaacgagcg gacacggcgg gcgggacggc tcggagttcg cgcgggaggc
     3001 cgagagctcg cagcccggca tcctgcgcga gttcggcgaa ttcctgctgc acaacaagaa
     3061 atggtggctg gcaccgatcg tcatcatcct gttattgttc gggttgttga tcgtcttcgg
     3121 tggcaccagc gcggctccct tcatctacac tctgttctga tccgaatcgg aggagagcct
     3181 cgggcaagac gatggaatcc cagaccggca agacgctgtc ccactacaag atcctgcgcc
     3241 ggctcggcgc cggcggcatg ggcgtggtct acgaggcgga ggacggccgc ctcggccgac
     3301 gcgtggcggt gaagttcctg tccgaggagc tgcagaacga cctccccgcg ctcgagcgat
     3361 tccagcgcga ggcccgcgcg gcctcggcgc tgaaccaccc cgggatctgc acggtgttcg
     3421 ccatcgagca gttcgagcgg cagcacttca tcgtgatgga gctgctcgag ggccagacgt
     3481 tggcccagcg catcgggcag cagccgttcg agatcggcgc gttgctcgac gtcgggatcc
     3541 aggtcgccga cgcgctcgaa tcggcccact cgaagggcat cgtccaccgc gacctgaagc
     3601 cggtgaacct gatcgtcaac gagcgcgggc aggtcaagat cctggacttc ggcctggcca
     3661 aggtcgaggg cgcgccggcg gcgatcgcgt ccgacgcgtc ctcgtcgcag gtcgagaccg
     3721 cggtcggtcg cggtcagctc accgcggtcg ggaccgtgct cggcacgatg ttctacatgt
     3781 cgcccgagca ggcgcggggc cagctgaccg acgcgcgcac cgacctgttc tcactcggcg
     3841 tggtgctgta ccagatggcg gccggcgagc tgccgttcca gggcgacacg caggcggtgg
     3901 tattcgacgc gatcctcaac cgcgacccgc ggccgctggc cgaggtcaac ccggcgatgc
     3961 cgcaggcgct gggtcagatc ctcgacaagg cgctcgagag agaccgcaac ctgcgctacc
     4021 agaccgcgac cgagctcaag accgacctgc tgaggctgcg tcgcaagctc gactccgggc
     4081 gccgccatgc ggccgaggcg gccgaggcca agtcggcgcc gggtaagcgc gccaagcgct
     4141 cgatcgcggt gctctatttc gagaacctca gcggcgtcaa ggacgacgag tacctgcgcg
     4201 acgggatcac cgaggacatc atcaccgagc tgtcgaagat cgaggggctg aagatcaacc
     4261 cgcgatcgac cgtactggcc taccgcgaca agccggtgac ctcggcccag gtcggccagc
     4321 agctcggctc ggcctacgcg ctcgagggca gcctgcgccg cgcgggagac cggctgcgca
     4381 tcacgacaca gctcgtcgac accgagaccg gctaccccgt ttggtccgag cgctacgacc
     4441 gcgaaatgaa ggacgtgttc gaggtccagg acgagatcgc gtgcaagatc gccgaagcgc
     4501 tgcgcatcaa gctctcgccc aaggagcagg aggcgatcgc agccaagccg accgacaacc
     4561 tgcaggccta cgacctgtac ctgcgcgggc gcagctacgc ccggcgcctg acgcggcagg
     4621 acctcgagtt cgcgctgcag atgtacgaga acgcagtgac ccaggacccc cacttcgcgc
     4681 tggcctacgc cgcgatcgcc aacgtctgcg cctactacca cgcgcactac gcgcgcgagg
     4741 acgtgtggat ccaccgcgcg cgcgccgccg ccgagcgcgc gatggcgctg cagcccgacg
     4801 tgccggaggt gatggtcgcc caggcgtgga tcctctacgc caaggcgcag tacgacgaga
     4861 tgaccgcgat cgtgcgcaac gtgatcgcgc gcaaacgcga atgcgagggg gcctactacc
     4921 tgctgctgcg cgggctctac gcttccggca agtaccagga cgtggccaac gtggccgacg
     4981 aggcgctcga atcgagcggc accgactaca acgtctacgt accgatcatg aattcgctcg
     5041 gcgctctcgg caaagacgag gcgctgcgca acatccgcca gcgtgcgatc caggcgttcg
     5101 aaacgcacct gcgccaggtg ccggaggatg cgcgggcgcg catcctcgtc ggcgtcaact
     5161 acgccgccga gggtcgccac gacgacgccg tgcgcgagac gaacctggcg atgctgctgc
     5221 ggcccaacga ggcgatggtc cactacaacg cggcgtgcac cttctgcttg atggagcgca
     5281 agggcgaggc gatcgacgcg ctggccaagg cttggcgcaa cggcttcaag gacgccgact
     5341 gggcgcggcg cgacccggag ctggcgatcc tgcacggcga ccccgaattc gagcggctgt
     5401 acccggtgtc accgagcgac gcctgagccc ggctccggcg gcgcgccccg gacgccggcc
     5461 tacgtgtcgt ccgagctcgc gccgagccgc tgccgagcga acgccagctg cgcgttgccc
     5521 gcgacgttga agttggccgc ccggtcgaac tgctgcttgg cccgctcgcc gtccccctgt
     5581 ccccggtagg cgactccgag caggtagctg ttgaacgggt tctgcggatt ggaccggccg
     5641 atccgcgaga ccgcctcgtc ccagtcctgc tcctggatgg cgatgatccc ggccaactga
     5701 tgggcggccc agatctggaa ctggttccgg ttggcttccg cccgactctg atactccgac
     5761 aggtgctcct tcgcctccgg cagctgcccg ctccacgccg cgacgcgtcc cgcatcgtag
     5821 tgataggtca ggcgggccag gtccttgact tcctcgctcg tgttctgcgc ttcgtcgagc
     5881 agggcgaccg tttcctcgaa tcgcgcgcgg gcctcgtcga agcgacccat ctcgagcagg
     5941 atgttgccca tgaagttcac gtcggccgac atagcggcgt ggtcgccggc ggccgcggcg
     6001 aggtcgtact gccgctgcac ctgggtcagc gcgcccggaa aatcgccccc agcgatgtac
     6061 gagaccgcca tcgcccccag cgccgtgcga cgctggccgt cgttgagagc ctgatcgtac
     6121 aactgtcgca gacgcgcgcg cgcttcgtcc ggacgaccca gcagatcgag attggtcgcg
     6181 a
//
LOCUS       DASPGN010000475         6456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1492872, whole genome shotgun sequence.
ACCESSION   DASPGN010000475 DASPGN010000000
VERSION     DASPGN010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6456
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1492872"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VD788_13870"
     CDS             complement(<1..351)
                     /locus_tag="VD788_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77400.1"
                     /translation="MRREPALGTFSLVTLTLLAGCQAVPSTTPRAPLTDDGLLYVYLR
                     PLSAKLERLSIEIGSASAQATGGEEWPLELQLQRISSRHVGRERLLAVATLPAGSYRG
                     LTVRIAGARLAGTSG"
     gene            complement(498..1793)
                     /locus_tag="VD788_13875"
     CDS             complement(498..1793)
                     /locus_tag="VD788_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77401.1"
                     /translation="MRVARVTWIVFAGLVLLVATSTTQAFHDGGVAECAGCHSMHNPN
                     QGGGSGSDHLLIAYDESSVCLSCHITSSATPSSYHVATADSLLGPGMSPTQRTPGGDF
                     GWLKKTYSWAPSWNPSAVETEEGYTHGHNIVADDYGYIPDGINTIAPGGTMLSDQLGC
                     ESCHDPHGTFRRDESGSYVVPAIGTTVAPIVASGSYHNSPTPGAGEAVGSYRLLAGPG
                     YGDFGAGNLFTVDPPMAIAPSSYNRTEASTQTLVAYGQGMSDWCATCHPNFHTATAPG
                     AFLHPTNQSINTGTPSVRSIYNAYVSSGDLSGTQANSYLSLVPYEEVALTWSELQTLV
                     TNNTAQTNIPGPDDNEYVMCLSCHRAHASGWEYAVRWNAESEFLTYGGVYPGTDTSAP
                     SQFSRGRTSAEMQAAYYDRPPTEFATYQRSLCNKCHAKD"
     gene            complement(1849..2979)
                     /locus_tag="VD788_13880"
     CDS             complement(1849..2979)
                     /locus_tag="VD788_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77402.1"
                     /translation="MLGTNHLATVGTALALALGSFPCAAQGLRVSHLYNLSDFSGALP
                     FNASRVYSDPVWNEVYVLYQGRVRIFNAAGMEVFAFGDDDALGFPLDIAVAEDGSIFV
                     LSYDYSDPTVRTRYYVSHCNYRGEFLRRIEISGQPEQFASLQPDRMLYAGGRLHLLGT
                     RQLELVTIDRDGHYLSGIDFEPSILAPGESVDNLTINGLSIDDTGTIYFSVAVKFRVY
                     RMTPDGAVTAFGESGSSAGQFGVIGGLDTDGSGNIYVTDRVRNTVMVFDPEFRFLTEF
                     GYRHSGPDGLVRPQDVISGSSGKLYITQVGNRGIAVFQIDHDDSPAADPDRDAASTER
                     RCRSAKSSHLITREFQSTTMVSTFQRVNDSKRHGRPAANPRT"
     gene            complement(3149..4054)
                     /locus_tag="VD788_13885"
     CDS             complement(3149..4054)
                     /locus_tag="VD788_13885"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HXV77403.1"
                     /translation="MRRRSRHVLWLASCIATGGLLHGTDAVRLRAIQVIHAGPASIGL
                     AAPEGVACGDGRAWIADTGNGRLVELTAAADGYVATSVVELPELARPQRVRLAADGSF
                     FVLDGRQRRIGRVDRSGSFLGWVDPSGAEPLGRVVPRGFDLGRDGRLYILDIAGRRVL
                     VTDAAGRVERRIGFAASAAFLSDVAVDPRGTVFAVDSVGRRLFRAPTDASELSPLSET
                     LEGDLDFPTDLTADPLGRLLVSDRHGGGIVIVGFDGSFRGRQSSMGWRPGMLRYPSGL
                     CVNGNGELFVADTGNNRVQVFRVVE"
     gene            complement(4060..5331)
                     /locus_tag="VD788_13890"
     CDS             complement(4060..5331)
                     /locus_tag="VD788_13890"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXV77404.1"
                     /translation="MIRSPAARPSAVLVLVVLFSTAVSSEGPYRLKPGARGTVCVDCH
                     ADFEQTLARRHVHTPVAAGNCADCHDPHASAHGALLNVEPQRICADCHADVVPADAVS
                     VHAAVVGGSCTDCHDPHASDNPDVLLAAGNAVCAGCHDEMSAAVGAVEFRHAPVEQGC
                     VGCHDPHASSAGPFLLKQAAPRLCLDCHRVDTPGFRQSHENYPVAQSDCGSCHDPHGS
                     NRAGMMWNSVHAPVEAGQCDSCHAGASSEDPLRVNDDRFDLCGSCHSGEIDRATIDPH
                     VHWPVLDAKSCANCHSPHASKQDGLLSEPTTALCGGCHQDVIRRHERSVTKHAPVGDG
                     DCGACHAAHSSASPFLLADSSVEQVCGNCHDWQTHSSHPLGEDVVDQRNPNLSLGCLT
                     CHRTHGSPHEHLVHFDATGPLCVDCHTRFQR"
     gene            complement(5341..>6456)
                     /locus_tag="VD788_13895"
     CDS             complement(5341..>6456)
                     /locus_tag="VD788_13895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXV77405.1"
                     /translation="SCHDPHASSQPKLLAAAGNALCTECHADQGRKFSIEHAAVGQGD
                     GCLSCHEAHAAPHDGLLAQQSTELCFGCHETTRGELNVAEPHEPFTAGMCESCHDPHG
                     AETKGLLRNRTDKVCYDCHADAELAYAKSHTHRPVLTGACGSCHVAHGSDRAGLLAGE
                     PSALCRECHADHRADPTMLTVHAPFGSDDCLSCHDPHAADHAGMITAAETQLCAECHG
                     DVAASGASGGSRHRPVVDGRCSDCHEPHQSKLEGLLLASSPELCLACHEPMAQAMETQ
                     RVHSPAARDCMRCHEAHAADQTSLLTRQLQPLCAECHDVDDPEFSSAHLGIDPGIINC
                     NQCHSPHYSVDQKLFQARVHAPFGSGDCAECHVVNEP"
BASE COUNT         1015 a         2128 c         2278 g         1035 t
ORIGIN      
        1 cccgcttgtc cccgccagcc gggccccggc gatacgcacc gtcaaaccgc ggtaggatcc
       61 cgccggcagc gtggcgacgg cgagcagccg ctcgcggccg acgtgacgcg aggagatccg
      121 ctgcagctgc agctcgagcg gccattcctc gccgcccgtc gcctgcgcgc tggccgagcc
      181 gatctcgatc gacagccgtt cgagcttggc cgatagcggt cgcaggtaga cgtagagcag
      241 accgtcgtcg gtcagcggcg cgcggggcgt ggtcgatgga acggcctggc aacccgcgag
      301 cagcgtcagc gtgacgagcg agaacgtgcc cagtgccggt tcgcgtcgca tgtcggacct
      361 cgatcgagga tgggctgcgt cagaaccgtt cagcctagcc cagcgcgaga ggttcgagca
      421 agaccggttg tcgggtgccg ggcgaacgaa ggggctggcg cgggccagcc ccttcgtgga
      481 cgtgggcggt cagcagatca gtccttcgcg tggcacttgt tgcagagcga tcgctggtag
      541 gtcgcgaact ccgtcggcgg tcggtcgtag taggcggcct gcatctcggc cgaggtgcgg
      601 ccgcggctga actgcgacgg cgcggaggtg tcggtgcccg gatagacgcc gccgtaggtc
      661 aggaactcgc tctccgcgtt ccagcgtacc gcgtactccc aaccggacgc gtgggcgcgg
      721 tggcaggaca ggcacattac gtactcgttg tcgtcggggc ccgggatgtt ggtctgggcc
      781 gtgttgttgg tcaccagcgt ctgcagctcg gaccaggtca acgccacctc ctcgtaaggc
      841 acgagcgaga ggtaggagtt ggcctgcgtg ccgctcagat caccgctgct gacgtacgcg
      901 ttgtagatcg agcggacgct cggcgttccc gtgttgatcg actggttggt cgggtggagg
      961 aacgcgccgg gtgccgtcgc ggtgtggaag ttggggtgac aggtggcgca ccaatcgctc
     1021 atcccctgac cgtaggcgac cagcgtctgc gtcgaggcct cggtgcggtt gtagctcgac
     1081 ggtgcgatcg ccatcggcgg atcgacggtg aacaggttac cggcgccgaa gtcgccgtaa
     1141 cccggtccgg caagcagccg atacgagccg accgcctcac cggcgccggg cgtcggtgag
     1201 ttgtggtacg aaccggaggc gacgatcggc gccacggtcg tcccgatcgc cgggacgacg
     1261 tagctgccgc tctcgtcacg ccggaaggtg ccgtgcgggt cgtgacagct ctcgcagccg
     1321 agctggtcgg acagcatcgt gccgccgggc gcgatcgtgt tgatcccgtc cgggatgtaa
     1381 ccgtagtcgt cggcgacgat gttgtgcccg tgcgtgtagc cttcctcggt ctcgaccgcg
     1441 ctcgggttcc agctcggagc ccagctgtac gtcttcttca gccagccgaa gtcgccgccg
     1501 ggcgtccgct gcgtcgggga catgccgggg ccgagcagcg agtcggccgt ggcgacgtga
     1561 tagctgctgg gcgtcgccga agaggtgatg tgacagctca gacagaccga gctctcgtcg
     1621 taggcgatca gcaggtggtc actgccgcta ccgccgcctt ggttgggatt gtgcatgctg
     1681 tggcagccgg cgcactcggc gacgccgccg tcgtgaaacg cctgggtcgt gctcgttgcg
     1741 acgagcaaca ccaacccggc gaacacgatc catgtgactc ttgcgactct catcctgttc
     1801 tcctcactcc ggcaacccgg ccgtcgcgac cgtcagggat ccgaagactc acgtcctcgg
     1861 attcgcggcg ggtcttccgt gtcgtttgga gtcgttcact cgttgaaacg ttgaaaccat
     1921 agtcgtggat tggaattctc gagtaatgag atgcgagctc ttcgctgaac ggcacctcct
     1981 ttcggtgctt gcggcgtccc gatccgggtc cgcggccggt gaatcgtcgt gatcgatctg
     2041 gaacacggca atgccccgat tgccgacctg cgtgatgtag agctttccgg agctgccgct
     2101 gatcacgtcc tgcggccgaa cgagcccgtc gggcccgctg tgacgatagc cgaactccgt
     2161 caggaagcgg aactcgggat cgaacaccat gaccgtgttg cgcacccggt cggtgacgta
     2221 gatgttgccg ctgccatcgg tgtcgagacc gccgatgacg ccgaactgtc ccgccgacga
     2281 gccgctctcg ccgaaggcgg tgacggctcc atcgggggtc atgcggtaga cgcggaactt
     2341 gacggcgacg gagaagtaga tcgtacccgt gtcgtcgatc gaaagaccgt tgatcgtcag
     2401 gttgtccacg ctctccccgg gcgcgaggat cgagggctcg aagtcgatgc cggagagata
     2461 gtggccgtcc cgatcgatcg taacgagctc gagctgccgc gtgccgagca ggtgcagccg
     2521 cccgccggca tagagcatgc gatccggttg cagcgatgcg aattgctcgg gttgtccgct
     2581 gatctcgatg cgtcgcagga actccccgcg gtagttgcag tggctcacgt aatagcgcgt
     2641 gcgcacagtc gggtcggagt agtcgtagga caacacgaag atcgacccgt cctcggcgac
     2701 ggcgatgtcg agcgggaagc cgagcgcgtc gtcgtcgccg aaggcgaaga cttccatccc
     2761 ggccgcgttg aagatgcgga cccgcccctg gtacagcacg tagacctcgt tccacaccgg
     2821 gtcgctgtag acccgcgagg cgttgaacgg cagcgctccc gagaagtcgg acaggttgta
     2881 caggtgcgag actcgaagcc cttgggccgc gcacgggaac gaccccagcg ccagggccag
     2941 ggccgttccg acagtcgcaa gatggttcgt tccaagcatc gggattcaga tctcgcaacc
     3001 ctcgtgccac tccggcacgg ttagacaacc tatttacgat gagacactta ggtacgcaat
     3061 ccggcgagac cggccgtcgg gtgcgggaga acgctcagtt gcgaccgggg ggtcgagcgt
     3121 gaaatcccca acgaaaccgg cccccgggtc actcgacgac gcggaagacc tgcacccggt
     3181 tgttgccggt gtcggcgacg aacagctcgc cgtttccgtt gacgcacagc ccggaaggat
     3241 agcggagcat gccgggacgc catcccatcg acgactgccg gccgcggaac gagccgtcga
     3301 agccaacgat gacgatcccg ccgccgtggc ggtcgctgac gagcagacgc cccagcgggt
     3361 ctgccgtcag gtcggtcggg aagtccaggt cgccctcgag cgtttcggac agcggactca
     3421 gctcgctcgc gtcggtcggg gcgcggaaca gccgccggcc gacgctgtcg acggcgaaga
     3481 ccgtcccgcg cgggtcgacc gccacgtccg agaggaacgc cgcgctcgcg gcgaagccga
     3541 tccggcgctc gacgcgaccg gcggcgtcgg tcacgagcac ccggcggccc gcgatgtcga
     3601 gtatgtacaa gcggccgtcg cgccccaggt cgaacccgcg cggtacgacc ctgccgagcg
     3661 gctcggcgcc ggacgggtcg acccagccca ggaagctccc cgatcgatcg acccgtccga
     3721 tgcgccgttg cctgccgtcg agtacgaaga acgatccgtc cgccgcaagc cgcacgcgtt
     3781 gcggtcgtgc caactccggc agctcgacga ccgaagtcgc cacgtagcca tcggccgccg
     3841 cggtcagctc gaccaggcgt ccgttgccgg tgtcggcgat ccacgcacgc ccgtcgccgc
     3901 aggcgacccc ctccggggcg gcgagaccga tcgaggcggg gccggcgtgg atcacctgga
     3961 tcgcgcgtag gcgcaccgcg tccgtgccgt gaagcaggcc gcccgtcgcg atgcacgacg
     4021 cgagccacag gacgtgtcgc gaccgccgcc gcatcaccat caccgctgaa accgcgtgtg
     4081 gcagtcgacg catagcggcc cggtcgcgtc gaaatggacg agatgctcgt ggggtgagcc
     4141 gtgggtccga tggcaggtca gacaacccag cgacaggttc gggtttcgtt ggtcgacgac
     4201 gtcctcaccg agcggatgcg acgaatgggt ttgccagtcg tgacagttgc cgcagacctg
     4261 ctccacgctg ctgtcggcca acagaaacgg cgaggctgag gagtgcgcgg cgtggcacgc
     4321 cccgcaatcg ccgtcgccga ccggggcgtg tttggtcacc gaccgctcgt ggcgccggat
     4381 gacgtcctgg tggcagcccc cgcacagcgc tgtcgtcggc tcgctcagca ggccgtcctg
     4441 cttcgacgcg tgcggcgagt gacagttcgc gcacgacttg gcgtccagga ccggccagtg
     4501 cacgtgcggg tcgatcgtcg cccggtcgat ctcgcccgaa tgacagctgc cgcacaggtc
     4561 gaatcgatcg tcgttgacgc gcagcggatc ctcggacgac gcaccggcgt gacacgagtc
     4621 gcattgaccc gcctcgaccg gcgcgtgcac gctgttccac atcatcccgg cccggttcga
     4681 cccgtgcggg tcgtggcacg agccgcagtc ggattgcgcg acggggtagt tctcgtggct
     4741 ctggcgaaag ccgggtgtgt cgacgcgatg gcagtcgagg cacagccgcg gggcggcctg
     4801 cttcagcagg aacgggccgg cgctcgaggc gtgcggatcg tgacagccga cgcacccctg
     4861 ttcgaccggc gcatgtcgga actcgaccgc cccgaccgcc gccgacatct cgtcgtgaca
     4921 gccggcgcag acggcgttgc ccgcggcgag caacacgtct ggattgtccg aggcgtgcgg
     4981 gtcgtggcag tcggtgcaac tcccgccgac gaccgccgcg tgcacgctga ccgcgtcggc
     5041 cggaacgaca tcggcgtggc agtcggcgca tatccgctgg ggctcgacgt tgagcaacgc
     5101 gccgtgtgcc gaggcgtgcg ggtcgtggca atcggcgcag ttgcccgcgg cgaccggcgt
     5161 gtggacgtgg cgtcgcgcca acgtctgctc gaaatcggca tggcagtcga cacagaccgt
     5221 gccgcgggca ccgggcttca accggtaggg cccctccgac gagacggcgg tcgagaacag
     5281 cacgaccagt acgagaacgg cggacggtcg tgctgcgggc gaacggatca tcaccgacct
     5341 ctacggctcg ttgaccacgt ggcactcggc acaatctccg ctgccgaacg gcgcgtggac
     5401 ccgtgcctgg aacagctttt ggtcgaccga gtagtgcggg ctgtgacact ggttgcagtt
     5461 gatgatcccc gggtcgatcc cgaggtgcgc cgacgagaac tccggatcgt cgacgtcgtg
     5521 acactcggcg cacaacggct gaagctgacg ggtcagcagg ctcgtctggt cggcggcgtg
     5581 cgcttcatga caccgcatgc agtcgcgcgc cgccggggaa tgcacgcgtt gggtttccat
     5641 cgcctgtgcc atcggctcat gacacgcgag gcacagctcg ggactggacg cgagcagcag
     5701 gccctcgagc ttcgactggt gcggctcgtg gcaatcgctg caccggccgt cgacgacggg
     5761 gcggtggcgg ctgccgccgc tcgcgccgct cgcggcgacg tcgccatgac actccgcgca
     5821 cagctgggtc tcggcggcgg tgatcatccc cgcgtgatcc gcggcgtggg gatcgtgaca
     5881 gctcaggcag tcgtccgacc cgaacggggc gtggacggtg agcatcgttg ggtcggcccg
     5941 atggtcggcg tgacactcgc ggcagagggc cgacggctcg ccggcgagca gcccggcccg
     6001 gtcgctgccg tgagccacgt ggcagctccc gcacgcgccc gtcagcaccg ggcggtgggt
     6061 gtggctcttc gcgtaggcca gctcggcgtc ggcgtggcag tcgtagcaga ccttgtcggt
     6121 gcggttgcgg agcagtccct tcgtctccgc cccgtgaggg tcgtggcacg attcgcacat
     6181 accggcggtg aacggctcgt gcggctccgc gacgttcagc tccccgcgcg tcgtctcgtg
     6241 gcagccgaag cacagctcgg tcgactgctg cgccagcaga ccgtcgtgtg gcgcggcgtg
     6301 cgcctcgtga caggacaggc acccgtcgcc ctggccgacc gcggcgtgct cgatcgagaa
     6361 cttccgacct tggtcggcgt gacactcggt gcacagcgcg ttccccgcgg cggccaacag
     6421 cttcggctgg gaggacgcgt gcggatcgtg gcagct
//
LOCUS       DASPGN010000476         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_791109, whole genome shotgun sequence.
ACCESSION   DASPGN010000476 DASPGN010000000
VERSION     DASPGN010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791109"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..1844)
                     /locus_tag="VD788_13900"
     CDS             complement(327..1844)
                     /locus_tag="VD788_13900"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXV77406.1"
                     /translation="MKRLPMLLWLACGSAAVAAVPGPAARPACPSETAEFPSYRSIEG
                     NVSFWKRVFGEWSTTRVVVHDIDHPAVVYEVADLPGPAAEQYSAEQREFVERLDERWR
                     QSLRGLSRKVTDGTTLDEEEKRVALVVATGAGTDALTGAADRVRTQRGMRERFRRGLE
                     LAQRYDAAIRRILRERGLPEELVFLPHVESSYRTDARSTAGAVGAWQFTRGAGRRFLR
                     VGPALDERLDPILAAYGAADYLADAHRQLGDWALALTAYNHGVNGMARAVALHGGDYQ
                     QVLRHYSGRSFGFASKNFYAEFLAAGEIACDPVRYFPEGLSPESPLDHERVALGERTT
                     AERIAESYGVPLDRLAAINPAWTPRAIESRAPLPAGSSVWLPAGSLADLARRGIGIEA
                     AWERVPEDDGVYVVRSGDTLTSIARRHGMTVDRLRAVNGMTNAQSVIHAGQRLRLSAG
                     DEVLDAVVHVVRSGETLLRIATIYGVRLGDLLALNGLTVDSVIHPGQRIRLPLGL"
     gene            1903..>2090
                     /locus_tag="VD788_13905"
     CDS             1903..>2090
                     /locus_tag="VD788_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77407.1"
                     /translation="MNELRRGQRVRSSRAACGAPGRDALRCAGEPHRSGASRMIQAAV
                     GISTETNTVRAVVEAARQA"
BASE COUNT          314 a          758 c          731 g          287 t
ORIGIN      
        1 gaccgccgcg ctcacggcgg cctccgagtc gtcgacgatc gtgattcgac ggacgaccgc
       61 gccgcgcccg gccagcgttt cggcaatcag cttcgcgttg gcgtcggcca gacgtccgcg
      121 cagcaattcg cgaccgacgg cgacgacctc gacgtgcatc ggaccgctcg ggtccggccg
      181 gaccttcggc ccgtcgacgg caccgagtcc cgccgcttcg atcggtcgcc tccgcctgcc
      241 ttccacgatc gcaccctgcg tcccgcgccc gcgggacggc ccgacagact atagcccata
      301 cggattgccg ggtccccgcc cgccggtcaa agaccgagcg gaagccggat gcgctggccg
      361 gggtgaatca ccgaatcgac ggtcagcccg ttgagcgcga gcagatcgcc caggcgaacg
      421 ccgtagatcg tcgcgatccg cagcaacgtc tcgccgctgc gcaccacgtg aacgacggcg
      481 tccagcacct cgtcgccggc ggacagacgc agccgctggc cggcgtggat cacgctttgg
      541 gcgttggtca tcccgttgac ggcgcgaagc cgatcgacgg tcatgccgtg gcgcctcgcg
      601 atcgaggtca gcgtgtcgcc gctgcggacg acgtagactc cgtcgtcctc cggcacgcgc
      661 tcccaggcgg cctcgattcc gatgccgcgc cgcgcgagat cggcgagtga gccggccggt
      721 agccagacgc tgctgccggc cggcagcggc gcccttgact cgatcgcccg cggggtccac
      781 gccgggttga tcgccgccaa ccggtcgagc ggcacgccgt aggactccgc gatccgctcg
      841 gcggtggtgc gctccccgag cgcgactcgc tcgtggtcga gcggtgattc cgggctcaac
      901 ccctcgggga agtagcggac gggatcgcag gcgatctcgc ccgccgcgag gaactcggcg
      961 tagaaattct tcgaggcgaa cccgaacgat ctgccggagt agtggcgcaa gacctgctgg
     1021 tagtctccgc cgtgaagcgc caccgcccgg gccattccgt tgacgccgtg gttgtaggcg
     1081 gtgagcgcca gggcccaatc gccgagctgt cggtgcgcgt cggcgagata gtcggcggcg
     1141 ccgtatgcgg cgaggatagg atcgagacgc tcgtcgagcg cggggccgac gcggagaaag
     1201 cggcggccgg cgccgcgggt gaactgccat gcgcccaccg cccccgcggt cgagcgagca
     1261 tcggtgcggt aggacgattc gacgtgcggc aggaagacga gctcctcggg cagaccgcgc
     1321 tctcgcagga tccggcggat cgccgcgtcg taacgctggg cgagctcgag cccgcgacga
     1381 aaccgctcgc gcatcccgcg ctgggttcgc acgcggtcgg cggcgccggt cagcgcgtcg
     1441 gtccccgcac ctgtcgccac gaccagcgcc acacgctttt cttcctcgtc gagcgtggtc
     1501 ccgtcggtca ccttgcgcga cagcccgcgc aagctctggc gccagcgttc gtcgagtcgt
     1561 tcgacgaact cgcgctgttc cgcgctgtac tgctccgcgg ccgggccggg caggtcggcg
     1621 acctcgtaga cgaccgccgg gtgatcgatg tcgtgcacca cgacccgcgt cgtgctccac
     1681 tcgccgaaga cgcgtttcca gaaggagacg ttgccctcga tcgaccggta cgacggaaac
     1741 tcggccgtct ccgacgggca cgcgggccgg gcggccggac ccgggaccgc ggccacggcc
     1801 gccgacccac aggcgagcca aagcagcatg ggaagccgct tcatcgatcc gactccgacg
     1861 aacgacccgt gccacccgcg acgcgcgccg agcttgaagt atatgaacga actccggcgc
     1921 ggacaacgcg tccgatcgtc ccgcgcggcc tgcggcgcgc ccgggcggga tgcgctacgt
     1981 tgtgccggag agccacaccg gtcgggggcc agccggatga tccaagccgc ggtcgggatc
     2041 tcgaccgaga cgaacacggt acgtgccgtc gtcgaggcgg cgcgccaggc
//
LOCUS       DASPGN010000477         5076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_323940, whole genome shotgun sequence.
ACCESSION   DASPGN010000477 DASPGN010000000
VERSION     DASPGN010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323940"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VD788_13910"
     CDS             complement(<1..404)
                     /locus_tag="VD788_13910"
                     /inference="COORDINATES: protein motif:HMM:NF036634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77408.1"
                     /translation="MHDRATPAVDSAFALSIGRGIPDELPEHPGFDDRVSHAPSRRPV
                     LDETERALAVANALRYFPTRLHPRLAPEFADELDRFGRIYMYRYRPTSYEMKARPLAS
                     YPANSRQAAAIMLMIQNNLDPAVAQHPHELVT"
     gene            complement(430..1890)
                     /gene="phrB"
                     /locus_tag="VD788_13915"
     CDS             complement(430..1890)
                     /gene="phrB"
                     /locus_tag="VD788_13915"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF007955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HXV77409.1"
                     /translation="MHGMVWFRSDLRLRDNTALDHACRRAERVSAVFLTAPEQWREHD
                     WGATKVDFVMRNVRELASSLAERGIALHLLAAPRFADAPAVLVELMREQGCGALFFNR
                     EHEVNERRRDRAVRVAVEAWGAAVEEYDDQTILAPDRVRTKTGGFYRVFGPYRRAWLE
                     ELDRGSRPTPRGLPAHPASPPVDRPRLPRQVAGYGPSPVPAGLWPAGEREARRRLDAF
                     VAGAIDRYHVDRDFPAVDGTSALSPYLAVGAVSARQCLAAASDANTGWVDGGRPGVAA
                     WIGELVWREFYRHVLVGFPRVSKNRPFRSETDEIEWRDDDESFRRWAAGATGVPIVDA
                     AMRQLHRTGWMHNRLRMVVAMYLTKHLLLDWRRGERHFMERLIDGDLANNNGGWQWSA
                     STGCDAAPYFRIFNPVRQSRRFDPQGRFILRFCPELRDLSGNALHDPSILPAERRARL
                     DYPPALVDHRSARARAIAAFHKLASSPRVRRAERSV"
     gene            complement(1947..2870)
                     /locus_tag="VD788_13920"
     CDS             complement(1947..2870)
                     /locus_tag="VD788_13920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HXV77410.1"
                     /translation="MTAGAGTRHVAVSGSSGLIGSALCAALAAAGDRVSRLVRDDGAS
                     SRPDEIVFLPEAGRIDRSRLRGVDAVVHLAGEGIAAGRWTAERKRRIRDSRVRGTRLL
                     ATALAGLEAGPEVLISASAIGFYGDRGDDELDEASEPGEGFLPETCVEWEGETAVAAR
                     AGVRVVLLRSGLVLSARGGALAKMLGPFRLGLGGRLGHGGQFVSWIALRDVIAILLWL
                     LADRAISGPVNAVAPTPVRNEEFTRVLAGVIGRPAIIPAPAFVLRALLGEMGRELLLA
                     STRVYPARLERAGYRFLDPELDGALRYELGR"
     gene            complement(2884..4023)
                     /locus_tag="VD788_13925"
     CDS             complement(2884..4023)
                     /locus_tag="VD788_13925"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HXV77411.1"
                     /translation="MRTSEVALCLLLSAAGLVAPAGQAGRAEWTVARPDYVWSFPRDH
                     WRHPGYRLEWWYFTGHLTTEEVPQREFGYQFTLFRIGLGPTPPALDSSWSASELLMGH
                     AAITDKHEKRHVFSEVLYRASGLLAGFNDHPQAEIAWSRAPVGTDGRWRLGWNGEAFD
                     FQMSDTGRGIAFELTTRPLKPLVFQGPNGFSRKSLAAGAASQYYSMTRLETRGTLSFD
                     GKRYDVHGSSWMDQEFSTSHLAEQQVGWDWFSLQLDDGRELMLYVMRRADGTIDHGRG
                     TLVTVGGSPIWLEPSRWDLATTADWTSRESGARYPAGWTIELPEFDIAVDVRPDVADQ
                     ENVGRLAGGLYYWEGVVTVRDRDGARVGRGYVELTGYGDGNRPPV"
     gene            complement(4010..>5076)
                     /locus_tag="VD788_13930"
     CDS             complement(4010..>5076)
                     /locus_tag="VD788_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXV77412.1"
                     /translation="LVGVTLMIGSFRRTVEIWIDSTLAADVYVTTESWSRARQTATLD
                     PALVERLARHDGVEAVDRLRQFFGYSGSRRISLVGVDMTIPAERARFELIDGDLDQAL
                     RRAVADGAVIVSEPLARKAGLAPGDELPVQTGSGEVRLPIVGVSYDYSNESGGAAMHL
                     ATMERLFGPGPINNVALYLAPGVDPARTIDGLRSSFAELPLVIRSNAEIRDQVFRIFD
                     QTFAVTRLLQLMSLAIAACGITLTLIVLARERISELALYRALGASRAQIFRVFLGKGL
                     GIALFGTMLGALGGLGLALILILRVNRAYFGWTIAVHWPIAELIEEAATILLAALLAS
                     LYPALRASRTPATELTRENL"
BASE COUNT          792 a         1860 c         1724 g          700 t
ORIGIN      
        1 taggtcacca gctcgtgcgg gtgctgcgcc accgccggat cgaggttgtt ctggatcatc
       61 agcatgatcg cggcggcctg gcggctgttg gccggatagc tcgccagcgg gcgcgccttc
      121 atctcgtagg aggtcggtcg atagcgatac atgtagatgc ggccgaagcg gtcgagctcg
      181 tcggcgaact cgggggccaa ccgtggatga agccgagtcg ggaaatagcg cagcgcgttg
      241 gccacggcca gcgctcgctc cgtttcgtcg agcaccggac ggcgcgacgg ggcgtggctg
      301 acgcgatcat cgaagcccgg gtgttccggc aactcgtcgg gaataccccg cccgatcgac
      361 agcgcgaacg cggaatcgac ggccggtgtc gctcgatcgt gcatggctcg agcctccccg
      421 ctcatcctat cagacggaac gctccgcccg acgaaccctc ggcgagctcg cgagcttgtg
      481 gaaggcggcg atcgcccgcg cgcgggccga ccggtgatcg accagcgccg gcggataatc
      541 gagcctcgct cgccgctcgg cgggtaggat cgacgggtcg tgcagcgcgt tgccggacag
      601 gtctctgagc tcgggacaga accgcaggat gaagcgacct tgggggtcga aacgccggct
      661 ctgacgtacc gggttgaaga tgcggaagta cggcgccgcg tcgcaaccgg tcgacgccga
      721 ccactgccag ccgccgttgt tgttggcgag gtcaccgtcg atcagccgtt ccatgaagtg
      781 acgttcgccg cgccgccagt cgagcagcag gtgcttggtc aagtacatcg cgacgaccat
      841 gcgcaggcga ttgtgcatcc agccggtgcg gtgcagctgg cgcatcgcgg catcgacgat
      901 cggaacgccg gtggcgccgg cggcccaacg ccggaacgac tcgtcgtcgt ctcgccattc
      961 gatctcgtcg gtctcgctcc tgaacggtcg gttcttcgac acgcgaggaa acccgaccag
     1021 cacgtggcgg tagaactcgc gccagaccag ttcaccgatc caggccgcga cgcccgggcg
     1081 cccgccgtcg acccagccgg tgttggcgtc gctcgcggcg gccagacact ggcgcgccga
     1141 gaccgcgccg acggcgaggt acggcgacaa cgcactggtg ccgtcgaccg ccgggaagtc
     1201 gcgatcgacg tggtagcggt cgatcgcccc ggcgacgaac gcgtcgagcc gccgccgcgc
     1261 ttcgcgctcg ccggccggcc acaacccggc aggaaccggc gacggcccgt accccgcgac
     1321 ctggcgcggc aggcgcgggc gatcgaccgg cggcgaggca gggtgtgccg gcaacccgcg
     1381 cggcgtcggg cgcgagccgc ggtcgagctc ttcgagccag gcacgccggt acggaccgaa
     1441 cacccggtag aacccgcccg tcttcgtgcg cacccggtcc ggcgccagga ttgtctggtc
     1501 gtcgtactcc tcgaccgcgg cgccccacgc ctcgacggcg acccgcacgg cgcggtcgcg
     1561 gcgccgttcg ttcacctcgt gctcgcggtt gaaaaacagc gctccacagc cctgttcgcg
     1621 catcagctcg acgagcaccg ccggcgcgtc ggcgaatcgc ggcgcggcca gcaggtgcag
     1681 cgcgatcccg cgttcggcga gcgaagaagc cagctcacgg acgttgcgca tgacgaaatc
     1741 gaccttggtc gcgccccagt cgtgctcgcg ccactgctcg ggggcggtca ggaagaccgc
     1801 gctcacccgc tcggcgcgcc gacaggcgtg gtcgagcgcg gtgttgtcgc gcaggcgcaa
     1861 gtcggagcgg aaccagacca ttccgtgcat cggggccctc tcggcaggtc attgttccgc
     1921 gccgcgtgcg cgctggcaaa ccacgctcag cggccgagct cgtaccgcag cgcgccgtcg
     1981 agctcggggt cgaggaagcg atagccggcg cgctcgagcc gcgcgggata gacccgtgtg
     2041 ctggccaaca gcagctcgcg gcccatctcg cccagcaacg cccgaaggac gaacgccggc
     2101 gccgggatga tcgccggccg cccgatgacg ccggccagca cgcgggtgaa ctcctcgttg
     2161 cgaaccggcg tcggcgccac tgcgttgacc ggaccggaga tcgcgcgatc ggcgagcagc
     2221 cacagcagaa tcgcgatcac gtcgcgcagg gcgatccagc tgacgaactg gccgccgtga
     2281 cccaggcggc cacccagccc cagccggaac ggcccgagca tcttggccag cgcgccgccg
     2341 cgcgcgctca acaccagacc ggagcgcaac agcacgaccc gcacaccggc ccgcgccgcg
     2401 accgccgtct cgccctccca ctcgacgcag gtctcgggaa ggaagccttc gcccggctcg
     2461 ctcgcctcgt ccagctcgtc gtcgccgcgg tcgccgtaga agccgatcgc cgaggcgctg
     2521 atcagcacct ccggtcccgc ttcgagcccg gccagcgccg tcgccaacag ccgcgtgccg
     2581 cgcacccggc tgtcacggat gcgacgcttg cgctcggcgg tccagcggcc ggcggcgatc
     2641 ccctcgccgg ccaggtgcac caccgcgtcg acaccgcgca gccgcgagcg gtcgatccga
     2701 ccggcttcgg gcagaaagac gatctcgtcc ggtcgcgagc tcgcgccgtc gtcgcgcacc
     2761 aaccggctga cgcggtcacc cgccgcggcc agcgcggcgc acagcgcgct gccgatcaga
     2821 ccgctcgagc cgctcaccgc gacgtgacgc gtaccggcgc cggccgtcat ccgagcctcg
     2881 cgttcagacg ggtggacggt tgccgtcgcc gtaccccgtc agttccacgt aacctcgccc
     2941 gacccgcgct ccgtcgcggt cgcgcaccgt taccactccc tcccaatagt acagcccgcc
     3001 cgccaaccga ccgacgttct cctggtccgc gacgtcgggc cgcacgtcga cggcgatgtc
     3061 gaattcgggc agctcgatcg tccagccggc gggatagcgc gcaccgcttt cccggctggt
     3121 ccagtcggcg gtcgtcgcca ggtcccagcg gctcggctcg agccagatcg gcgatccgcc
     3181 gacggtcaca agcgttccac gtccgtggtc gatcgtcccg tcggcccggc gcatgacgta
     3241 cagcatcagc tcgcggccgt cgtccagctg caggctgaac caatcccagc cgacctgctg
     3301 ctcggcgaga tggctcgtgc tgaattcctg gtccatccag ctcgacccgt gcacgtcgta
     3361 ccgcttcccg tcgaacgaca gcgtcccccg cgtctcgagc cgcgtcatgc tgtagtactg
     3421 gctggcggcg ccggcggcga gcgactttcg gctgaagccg ttcgggccct ggaataccaa
     3481 cggcttgagc ggccgcgtcg tcagctcgaa ggcgatgccg cgcccggtgt cgctcatctg
     3541 gaaatcgaac gcctcgccgt tccaaccgag ccgccaccgt ccgtccgttc cgaccggcgc
     3601 gcgcgaccag gcgatctcgg cctgcggatg gtcgttgaac cccgcgagca gtccggaggc
     3661 gcgataaagc acctcgctga acacgtgccg tttctcgtgc ttgtcggtga tcgcggcgtg
     3721 gcccatcaac agctcggagg cgctccagct cgagtcgagg gcgggcggtg tcggaccgag
     3781 gccgatgcgg aacagggtga actggtagcc gaactcgcgc tgcggcacct cctccgtcgt
     3841 caggtgcccg gtgaagtacc accactcgag ccggtatccc gggtgccgcc agtgatcccg
     3901 cggaaacgac cagacgtaat ctggacgtgc gacggtccac tcggctcggc ccgcctggcc
     3961 ggccggtgcg acgagcccag cggccgacaa cagcagacag agcgcgacct cagaggttct
     4021 cacgcgtcag ctccgtcgcc ggcgtgcggc tcgcccgcag cgcggggtag aggctggcca
     4081 acagcgccgc cagcaggatc gtcgccgcct cctcgatcaa ctcggcgatc ggccagtgca
     4141 cggcgatcgt ccagccgaag tacgcgcggt tgacccgcag gatcaggatc agcgccaggc
     4201 cgagaccgcc gagcgcgccg agcatcgtgc cgaacagcgc gatgcccaac cccttgccga
     4261 ggaagacccg gaagatctgc gcgcgcgagg cacccagcgc gcggtacagc gccagctcgg
     4321 agatgcgctc ccgcgccagc acgatcagtg tcaaggtgat gccgcacgcc gcgatcgcca
     4381 ggctcatcag ttgcagcagt cgcgtgacgg cgaaagtctg gtcgaagatg cggaacacct
     4441 ggtcgcggat ctccgcgttg ctgcggatca cgagcggcag ctcggcgaag ctcgagcgca
     4501 ggccgtcgat cgtgcgcgcg gggtcgacgc ccggtgcgag atagagtgcg acgttgttga
     4561 tcggacctgg cccgaacagc cgctccatcg tcgccaggtg catcgcggcc ccgcccgact
     4621 cgttgctgta gtcgtaggag accccgacga tcggcagccg cacctcgccg ctgccggtct
     4681 gaaccggcag ctcgtcgccc ggcgcgagcc ccgccttgcg cgccagcggc tcgctgacga
     4741 tcaccgcccc gtcggcgacc gcgcgccgca gcgcctggtc gagatcgccg tcgatcagct
     4801 cgaagcgggc ccgctcggcg gggatcgtca tgtcgacgcc gaccagcgag atccgccggc
     4861 tcccgctgta gccgaagaac tggcgcaggc ggtcgaccgc ctcgacgccg tcgtgccgcg
     4921 ccagacgttc gaccagcgcc ggatcgagcg tcgcggtctg ccgcgcccgt gaccaggact
     4981 cggtcgtgac gtagacgtcg gccgcgagcg tcgagtcgat ccagatctcg accgtccggc
     5041 ggaagctgcc gatcatcaac gtgacgccga cgagca
//
LOCUS       DASPGN010000478         6304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_673894, whole genome shotgun sequence.
ACCESSION   DASPGN010000478 DASPGN010000000
VERSION     DASPGN010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673894"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="VD788_13935"
     CDS             <1..1531
                     /locus_tag="VD788_13935"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXV77413.1"
                     /translation="ADKADGYGIPGLSVDGTDPEAIAAAFAWAADRARAGGGPTLIET
                     ISMRMCGHAHHDDMLYLGREPQPGWDYPVPVDGGYVDRAAWEYWSAREPIRRYADRLR
                     AEGVIGNGDESTMREEVAALVAQQARWVVEAAWPAAEQAGAGVFAGEPIRRHVEPFAN
                     RTARLGAATPELPPLEPGPPFDRKGRTFLEAVMTGVGDALAADERVFVYGQDVGGLYG
                     NAFLLLRPLLERYGDRILNSPLSEGGVLGACIGAALAGSRPIAEIQFNDFVATGFNQL
                     VNNAAKIRYRWGGQVPMVVRMPCGGLRRAGPYHSQNTEPWFYRTPGLKIVVPSTPHDA
                     RALMATAVDDPDPVLYYEHIALYRNPKIKQALPAAAPEPIPLGRAALRRVGSDLALVS
                     YGAYVHVCLDVAERLAGDGIECAVLDLRSLVPLDREAVLRTARHCGKLLIAHEDTRTG
                     GIGESLAALVQEEAFEELDAPIRVVGALDTPVPYSPPLEDEFLPGIDRVEHAARLLAE
                     Y"
     gene            complement(1599..3086)
                     /locus_tag="VD788_13940"
     CDS             complement(1599..3086)
                     /locus_tag="VD788_13940"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV77414.1"
                     /translation="MRKSIVLLCLFGLAGCAPDLESPIREATGPAVVSGPLTLTPIRF
                     ATTDYFLRPAVVRAEEGTLRVPERHADPGGRQITVHFVRFATTAADPGPPIVYLAGGP
                     GGSGTWSSSGDRFPLFQRLREIADVIAYDQRGVWGTEPYLVCPDSWSYPLDRPEDEAV
                     LREAKRPFLEKCWKHWSSEADLSAYNTLESVEDLEALRRALGAEQLTLWGISYGTHLG
                     LAYIRMHPERVHRAILAGVEGPDHTYKLPARIDAVLHRVAEAIRADPRAVRVVPDFVG
                     ALDQALHRLEREPVTVAVKHPTTKQEHRVVVGANDLRRAVYGSLGEREDIVELVEHAL
                     PLLQGDYTELGLFALRSRLDNREIVMSASMDCASGATSARAELIREQGRTAILGDVIN
                     ARLRSTCAGWPVEDLGDAYRSQVAGSMPVLFLSGSLDVKTPPENAEDLLPGFPRGHHL
                     IIEGASHDDDLFLSSPRIADAIVGFLQGEPVVERVELAPLRFALP"
     gene            complement(3157..3963)
                     /locus_tag="VD788_13945"
     CDS             complement(3157..3963)
                     /locus_tag="VD788_13945"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXV77415.1"
                     /translation="MARRGVPLAMDAPDPDCYFRDKTLLAGEGHGPLAHAHHVVTLVV
                     HGRLDETFERGTQQCGRCELHYKPPGARHTTSAGSAGVRMLLLGMRGDTLDGLAMPEA
                     PCALGGGVPAARALHTFVSVAEACRLGRPVDRESVRRLVRCLERPARAHAGPRPGWIT
                     EAYGRVREQDARRTRLELLSREFGVHPIYLARAFRLHYGRSIGEVRRRTRVDRAVDRL
                     VSGSAPLAATAYELGYSDQSHFTREFKRETGWSPGWFRDAVAGWGRIPGA"
     gene            complement(4040..4732)
                     /locus_tag="VD788_13950"
     CDS             complement(4040..4732)
                     /locus_tag="VD788_13950"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HXV77416.1"
                     /translation="MGFDARRIVLRLNPSLRGRVRVGLVVAEPVSIRPAGAALAGLMR
                     ETERVMRERYAGRAPGEIEPLAAARELYRGFGIDPTKTRPSSEALLRRVLRGRELPSI
                     VNAVDLSNVLSVEFLLPIGLYDAARIDGEVELRAGGPGESYAGIRKHEVHLDGRPVLV
                     DRSGPFGNPTSDSLRTSVDGRTTSLWMTIFAPPSLSGATLARHVERARHSIALHLAAG
                     GAPVAACGTVVD"
     gene            complement(4754..5344)
                     /locus_tag="VD788_13955"
     CDS             complement(4754..5344)
                     /locus_tag="VD788_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77417.1"
                     /translation="MRFKICRTAAGWCAALTLGLSVTGTAPAQTADASGPPEPIQRHL
                     VKLSRLISTTENGLKGGPSQFRRQTDHVNATPAWSCCSNNLKAIEQSAVAVQSMLQQL
                     DNCYQDRELTDQLIQGRLVQTDLQSLAKAIQRFADAPTVELAQGMMPTVTRSYLNLTD
                     SAAELAECGELLDPSLVATSESETSGETSETPPRPD"
     gene            5479..>6304
                     /locus_tag="VD788_13960"
     CDS             5479..>6304
                     /locus_tag="VD788_13960"
                     /inference="COORDINATES: protein motif:HMM:NF039677.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6178 family protein"
                     /protein_id="HXV77418.1"
                     /translation="MATDDRESGELAKLAVELARLARDAPERLAQRVSRLTIREQAEL
                     ALRLPAAQRIELMLHAPKPMRLVRSLPDADLYLTVREVGPADAGSVLALASAAQLQHL
                     IDLEAWRGDRFDGDRAGAWIALLLEAGEPALRRFLRHADEELLVLLFQRWIRVVQIEY
                     EDGAEKHGHGEGEIGTEAGAITPDGYHRYSPSIAEHAPAIRRMLQVFFVDQPERYRQV
                     VWGALWEMSSDIEEQALYWRQSRLEEHGFPDREQALSVYAAPLGARVHRRSELPVPV"
BASE COUNT          965 a         2144 c         2234 g          961 t
ORIGIN      
        1 tgccgacaag gccgacggct acgggatccc gggtctgagc gtcgatggta ccgaccccga
       61 ggcgatcgcc gccgctttcg cctgggcggc ggaccgggcg cgcgcgggag gcgggccgac
      121 gctgatcgag acgatctcga tgcggatgtg cggacacgcc caccacgacg acatgctcta
      181 cctcggccgg gagccgcaac cggggtggga ctatccggtg ccggtggacg gcggatacgt
      241 cgatcgagcc gcgtgggaat actggtcggc gcgggagccg attcgacgct acgccgaccg
      301 cttgcgcgcc gagggcgtca tcggcaacgg cgatgaatcg acgatgcgcg aggaagtcgc
      361 ggcgctcgtc gcgcaacagg cgcggtgggt ggtcgaggca gcgtggcccg ccgccgaaca
      421 ggccggtgcc ggcgtgtttg ccggtgagcc gatccggcgc cacgtcgagc cgttcgccaa
      481 tcgcaccgct cggctcggcg ccgcgacgcc cgagctgccg ccgctcgaac cgggtccgcc
      541 tttcgaccgt aagggccgca ccttcctcga ggccgtgatg actggcgtcg gcgacgcgct
      601 ggccgcggac gagcgggtgt tcgtctacgg gcaggatgtc ggcgggctct acggcaacgc
      661 cttcctgctg ttgcgtccgc tgctcgagcg ttacggcgat cggatcctca actcgccgct
      721 gtccgagggc ggagtgctcg gcgcgtgcat cggtgccgcg ctggcggggt cgagaccgat
      781 cgcagagatc cagttcaacg actttgtcgc caccggattc aaccaactgg tgaacaacgc
      841 ggcgaagatc cgttatcggt ggggcgggca agtgccgatg gtcgtgcgca tgccatgcgg
      901 cggactgcga cgcgccggcc cgtatcacag tcagaacacc gagccctggt tctatcgcac
      961 gccggggttg aagatcgtcg tgccgtcgac cccccacgac gcccgggcgc tgatggcgac
     1021 ggccgtcgac gatccggatc ccgtgctgta ctacgagcac atcgcgttgt atcgcaatcc
     1081 gaagatcaag caggcgctcc cggctgcggc acccgagccg atcccgctcg gccgggcagc
     1141 gttgcgccgg gtcggatcgg acctggcgct ggtcagctac ggcgcctatg tccacgtttg
     1201 cctggacgtc gccgaacgac tggccggcga cggtatcgag tgcgccgtgc tcgacctgcg
     1261 cagcctggtg ccgctcgatc gcgaggccgt gctgcgaacc gcgagacact gcggcaagct
     1321 gttgatcgcc cacgaggaca cgcggaccgg cggcattggc gagagcctgg ccgcgctggt
     1381 gcaggaagaa gcgttcgaag agctggacgc tccgatccgc gtggtcggtg cgctcgacac
     1441 gcccgtgccc tactcgcctc cgctcgagga cgagttcttg ccggggatcg accgcgtcga
     1501 gcacgcggcg cggctgttgg ccgagtactg aggggggatc caccgagcag cgtcgaggtt
     1561 cgcgtggacc ccgctcgacc ggttccgagc ggtgacactc acggaagcgc gaaacgcagc
     1621 ggcgcgagct cgacgcgctc gacgaccggc tcgccctgca ggaagccgac gatggcatcg
     1681 gcgatccgtg gcgacgacag gaacaggtcg tcgtcgtgcg acgcaccctc gatgatcagg
     1741 tggtggccgc ggggaaaacc cgggagcagg tcctcggcgt tctcgggcgg cgtcttgacg
     1801 tcgaggctgc cgctgaggaa caacacgggc atcgagcccg cgacctgcga gcggtacgca
     1861 tcgccgaggt cctccaccgg ccacccggca caggtcgaac gcagcctggc gttgatcacg
     1921 tcgcccagga tcgccgtgcg tccctgctcg cggatcaact ccgcgcgcgc cgacgttgcc
     1981 cccgaggcgc agtccatcga agccgacatc acgatctcgc ggttgtcgag ccgcgagcgg
     2041 agtgcgaaca ggccgagctc cgtgtagtcg ccctgcagca gcggcagcgc gtgctcgacc
     2101 agctcgacga tgtcctcacg ctcgcccagc gaaccgtaga ccgctcgccg caggtcgttc
     2161 gcgccgacga cgacgcggtg ctcctgcttc gtcgtcgggt gcttcaccgc gacggtcacc
     2221 ggttcgcgct cgagccggtg gagcgcctgg tcgagtgcac cgacgaagtc gggaacgacc
     2281 ctgacggcgc gcggatcggc ccgaatcgct tcggccacgc ggtgcaggac ggcgtcgata
     2341 cgcgccggca gcttgtacgt gtgatcggga ccctcgacgc cggccaggat cgcacggtgc
     2401 acccgctcgg gatgcatccg gatataggcc agcccgaggt gcgtgccgta gctgatgccc
     2461 cacagcgtca actgctcggc gccgagagcg cggcgcaacg cctccaggtc ctcgaccgac
     2521 tccagtgtgt tgtacgcgga gaggtcggcc tcggagctcc agtgcttcca gcatttctcg
     2581 aggaacggtc gtttcgcctc gcggagcacg gcctcgtcct ccggccggtc gagcgggtag
     2641 ctccacgagt cggggcaaac caggtagggc tcggtgcccc acaccccacg ctggtcgtag
     2701 gcgatcacgt cggcgatctc gcgcaatcgc tggaacagcg gaaagcgatc gccggacgag
     2761 gaccaggtgc cgctaccccc gggcccgccg gcgagataga cgatcggcgg ccccgggtcg
     2821 gcggcggtgg tcgcgaaccg tacgaagtgc accgtgatct ggcgccctcc cgggtcggcg
     2881 tggcgctcgg gaacgcgcag cgttccctcc tccgcgcgca cgacggccgg acgcaggaag
     2941 tagtccgtcg tcgcgaagcg gattggcgtc aatgtcagcg gacccgacac caccgctggg
     3001 ccggtcgcct cgcggatcgg actctcgagg tccggcgcac aacccgcgag gccgaagagg
     3061 caaagcagga cgatcgactt acgcatccag gtggcacctc cgtgttcagc atccgccgat
     3121 ccgggcggag gatcttgaac gatccgaacc gggctctcag gccccgggga tgcggcccca
     3181 gccggcaacg gcgtcgcgga accatccggg cgaccagccg gtctcgcgct tgaactcgcg
     3241 ggtgaagtga ctctggtcgg agtaaccgag ttcgtacgcc gtcgccgcga gcggtgcgga
     3301 gccggagacg agtcggtcga ccgcgcgatc gacccgcgtg cggcggcgca cctcgccgat
     3361 cgagcggccg taatgcaggc ggaacgcgcg cgcgaggtag atcgggtgga caccgaattc
     3421 cctggagagt agctcgagcc gggttcgcct cgcgtcctgt tcgcggacgc gcccgtaggc
     3481 ttcggtgatc cagcccggcc gaggtccggc gtgcgcgcgg gctggacgct ccaggcagcg
     3541 caccagccgt cggaccgact cgcggtcgac gggcctgccg agccggcaag cctcggcaac
     3601 cgagacgaag gtgtgcagcg cccgggcggc cgggaccccg ccgccgagcg cgcacggagc
     3661 ctccggcatg gccaggccgt cgagcgtgtc cccgcgcatt ccgagcagca gcattcggac
     3721 gcccgccgaa cccgcggagg tcgtgtgccg agcgccggga ggcttgtaat gcagctcgca
     3781 gcgtccgcac tgctgggtac cgcgctcgaa ggtctcgtcc agccggccgt ggaccacgag
     3841 cgttaccacg tggtgcgcgt gtgccagcgg accgtgtccc tcgcccgcca gcagggtctt
     3901 gtcacggaag tagcagtcgg gatccggtgc gtccatcgcc aagggaacgc cccgccgggc
     3961 caatcggttt catccggcgc atcgacgtga ccggcgcgag ccgaccccgc ggatcgaccc
     4021 gacggtgacc ggtcgtggtt cagtcgacga ccgttccgca agcggcgacc ggggcgccgc
     4081 cggcggcgag atgaagagcg atcgaatgtc gggcgcgctc gacgtgtcgt gcgagcgtgg
     4141 cccccgacag cgacggcgga gcgaagatcg tcatccatag cgaagtggtt cgcccgtcga
     4201 ccgacgttcg cagcgaatcc gacgtcgggt tgccgaacgg tccgctgcgg tcgaccagca
     4261 ccggtcgccc gtcgaggtgg acttcgtgtt tgcgaatccc cgcgtaggac tcacctggac
     4321 cgccggcgcg cagctcgacc tcgccgtcga ttctcgccgc gtcgtacaga ccgatcggca
     4381 gcaggaactc gaccgacaag acgttggaca agtcgacggc gttgacgatg ctcggcagct
     4441 ctcggccgcg caagacgcgt cgcagcagtg cttccgacga cgggcgggtt ttcgtcggat
     4501 cgatgccgaa gccgcggtac aactcgcgcg cggcggcgag cggttcgatc tcgcccggcg
     4561 ccctgcccgc gtaccgctcg cgcatcacgc gttcggtttc gcgcatcagc ccggccaacg
     4621 ccgcgccggc cggccggatc gagacgggct ccgctacgac gagcccgacg cgcacccggc
     4681 cgcgaagcga cggattcagg cgaagcacga tccgtcgagc gtcgaagccc atctcgcttc
     4741 tccggacgcg ggtctaatcg ggtcgcgggg gggtctcgga cgtctcgccg gaagtctcgc
     4801 tctcgctcgt ggcgacgagc gacgggtcga gcagctcccc gcattcggcc agctcggcgg
     4861 cggagtcggt caggttgagg tagcttcgcg tcaccgtcgg catcattccc tgcgccagtt
     4921 cgacggtcgg cgcgtcggcg aatcgttgga tcgccttggc cagcgactgc agatcggtct
     4981 gtacgagtcg cccttggatc aactggtcgg tcagctcgcg atcctggtag cagttgtcga
     5041 gctgttgcag catgctctgg accgcgacgg cgctctgttc gatcgccttc agattgttgc
     5101 tacagcagga ccacgccggt gtggcgttca cgtgatcggt ctgccggcgg aactggctcg
     5161 gacctccctt cagaccgttc tccgtcgtcg agatcagtcg actcaacttg accagatgac
     5221 gttggatcgg ctccggcggc ccggatgcat cagccgtttg tgccggtgcg gtacccgtga
     5281 cggacagacc gagcgtcagc gcggcgcacc agccagccgc cgttcgacag atcttgaacc
     5341 gcatcgatcc tcccgcgtgc cggtcgctgc cgacgcaacc ggaagtatac cgtcggtcgc
     5401 ggcgaccgac agccgcgctc tgctatgatc cccgcggtca gccgtctttt tcgagatcgg
     5461 cggacgacgg gatcccgcat ggcgactgac gaccgagaga gcggcgagct ggcgaagctc
     5521 gcggtcgagt tggcccggct ggcgcgtgac gcgcccgagc gcttggcgca gcgcgtgtcg
     5581 cggctgacga tccgcgagca ggccgagctg gcgctgagac tgcccgccgc gcaacggatc
     5641 gagttgatgc tgcacgcccc caagccgatg cggctggtgc gctccctgcc ggatgcggat
     5701 ctctacctga ccgtacgcga ggtcggtccg gccgacgccg gatcggtgct ggccttggcc
     5761 tccgccgccc agttgcagca cctgatcgat ctcgaggcgt ggcgcgggga tcgcttcgac
     5821 ggcgaccggg ccggggcttg gatcgcgctg ttgctcgagg ccggcgagcc ggcgctacgc
     5881 cgctttctcc gccacgccga cgaggaactg ctggtgctgc tgttccagcg gtggatccgc
     5941 gtggtgcaga tcgagtacga ggacggcgcg gaaaagcacg gtcacggcga gggcgagatc
     6001 gggaccgagg ccggtgcgat cacccccgac gggtaccatc gctactcgcc gtcgatcgcg
     6061 gagcacgctc cggcaatccg ccgcatgctc caagtcttct tcgtcgatca gccggagcgc
     6121 taccggcagg tcgtttgggg cgcgttgtgg gagatgtcgt ccgacatcga ggagcaggcg
     6181 ttgtactggc gtcagagccg gctggaggaa cacggctttc ccgatcgcga acaggccctg
     6241 tcggtttacg cggcaccgct cggcgcgcgg gtgcatcgga gaagcgagct acccgtgccg
     6301 gtcg
//
LOCUS       DASPGN010000479         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_324190, whole genome shotgun sequence.
ACCESSION   DASPGN010000479 DASPGN010000000
VERSION     DASPGN010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324190"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..264)
                     /gene="thiS"
                     /locus_tag="VD788_13965"
     CDS             complement(55..264)
                     /gene="thiS"
                     /locus_tag="VD788_13965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HXV77419.1"
                     /translation="MGSTIIVNGESWPRELASIEVLLRELGHDPTRPGIAVAVNDEVV
                     PRREWPRRPLRFGDRVEIVGAVQGG"
     gene            429..1082
                     /locus_tag="VD788_13970"
     CDS             429..1082
                     /locus_tag="VD788_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77420.1"
                     /translation="MKRSVVVSCLFVLLAALVSAVALAWPAFFDLNPPGIGAPPSLSG
                     WECISQPYVNPLFDAGELMDDINAVAGGPVTTQVCRWLTTVDGFSCYTGTTGAPFPLE
                     AGPGYLVRVSGPAAIPYVIAGGDVPLFPLMLDGPGPTSASGTHLISIPEVDIPVPLAD
                     AGDVLALLGPPAESVCRFAREANAFLCYTGTAGAAFPLVDGEAYLVQVDAPVVVMMP"
     gene            complement(1101..3296)
                     /locus_tag="VD788_13975"
     CDS             complement(1101..3296)
                     /locus_tag="VD788_13975"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77421.1"
                     /translation="MTRRACWLLVSTIGAVACGKPVAPVAPAEPGYVGVAACVPCHRQ
                     QVEAWRGSHHDLAMQPATGETVLGDFDGASVAAHGVGSTFFTEDGQYRVRTDGPDGAP
                     ATFRVAYTFGVTPLQQYLIEMPGGRYQALGLAWDARPAGEGGQRWFHLYPADARDHRE
                     PLHWTGPQQNWNYMCAECHSTALARNYAPAEDRFDTTWSEVDVSCEACHGPASLHVDW
                     VERDAAARGRDAGRGLVAGLRSTDGGRWVFDGARPTARRSVPRSSDAELETCARCHSR
                     RGWVWEDYLPGRPLADTHRVALLEQGLYQPDGQILDEVYEYGSFLQSRMHAEGVTCSD
                     CHDPHSARLVLDGNALCSTCHAPATFDTPEHHHHAQGRAGTRCVDCHMPERKFMIIDP
                     RRDHGFRVPRPDLSVALGVSNACNDCHAERSPRWAATTVAGWFPEGRSGRFHYGTALH
                     AARIGHPDGPALLRRVVGDPAQPAIVRATALAEMWRAPVRDLAAVLGRAAADADPLVR
                     RVVAELAGEIDPDAGAALAAGLLDDPVRTVRLAAAASLAGTAADRLDADRRLALDRAI
                     AEYRESLEFDRFRAEAQLNLGNLERQLGHAAEAEAAYRRAHAADPSFAPAQINLADLL
                     ASLGRNTEGVSLLREAASEWPDSAELHHALGLALARERRNAEALEHLRRAASLLPGSP
                     RFAFVYGVALHDTGDLAGALEVLRAAAELHPHDREIRRALEIYDAERGR"
     gene            complement(3420..>3777)
                     /locus_tag="VD788_13980"
     CDS             complement(3420..>3777)
                     /locus_tag="VD788_13980"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXV77422.1"
                     /translation="SGVLDDAPYTFATRFENKPGTNPEELIAAAHAGCFSMALSAELG
                     KAGLTPTRIETTAAVTLETVDGKPTVTRSHLTVRARVPGAERAKFLEVADGAKAGCPI
                     SRLLDAEITMEATLES"
BASE COUNT          516 a         1373 c         1327 g          561 t
ORIGIN      
        1 tcgaccgcag cggaataccg gcaagctccc agccgtccgg ttcgtcgcgt tccatcatcc
       61 accctggacc gcgccgacga tctcgacacg gtcgccgaag cgcagcgggc gtcgcggcca
      121 ctcgcgtcgc gggacgacct cgtcgttgac cgcgacggcg atacccggcc gcgtcggatc
      181 gtgccccagc tcgcgcaaca acacctcgat cgacgcaagt tcgcgcggcc aggactcccc
      241 attgacgatg atcgtcgatc ccacggtcgc gctccccgag accattgtag cggaacggcc
      301 tcgtgctgcc ccggggccgt tcgggcggcg acctcgcgcg ggtccgaccg ccgaaactcg
      361 cggattgaca gtgccggccc gccatggtac aggagccgca acacattccg agcacgaggg
      421 gggcacttat gaaacggtcg gtcgtcgttt cgtgtctgtt cgtgttgctc gcggcgctcg
      481 tcagtgccgt cgcgctggcg tggccggcgt tcttcgatct caatccgccc ggcatcggcg
      541 cgcctccctc cctctcggga tgggagtgca tctcgcaacc gtacgtgaac ccgctattcg
      601 acgccgggga gctgatggac gacatcaacg cggtcgccgg tggcccggtc acgacgcagg
      661 tgtgccgctg gctgaccacc gtcgacggct tttcctgcta tacggggacc actggcgcac
      721 cgttcccgct cgaggcgggg ccggggtatc tggtgcgcgt gtcgggtccg gccgcgatcc
      781 cgtacgtgat cgcgggcggt gacgtcccgc tgttcccgct catgctcgac gggccggggc
      841 cgacctcggc cagcgggacg cacctcatct cgattccgga ggtcgacatc ccggtcccgc
      901 tggccgacgc aggcgacgta ctcgctctac tgggcccacc ggcggagtcg gtctgtcgtt
      961 tcgcgcgcga ggctaacgca ttcttgtgtt acacgggcac ggcgggcgcc gccttcccgc
     1021 tcgtcgacgg cgaggcctat ctcgtccaag tcgatgcgcc ggtcgtcgtc atgatgccgt
     1081 gacgcgcgca ccggaatcgg tcaacgacca cgctccgcgt cgtagatctc caaggcgcgg
     1141 cgaatctccc gatcgtgagg gtggagctcc gccgccgcgc gcaggacctc gagtgcgccc
     1201 gcgaggtcgc ccgtgtcgtg cagcgccacc ccgtagacga aggcgaagcg cgggcttccc
     1261 ggcagtagcg aagcggcgcg ccgcaggtgc tcgagggcct cggcgttgcg gcgctcccgc
     1321 gccagggcga gcccgagggc gtggtgcagc tcggccgaat cgggccactc ggacgcggcc
     1381 tcgcgcaaca ggctcacacc ctcggtgttt cgtccgagcg aggcgagcag gtcggccaga
     1441 ttgatctgcg cgggcgcgaa cgacgggtcg gcggcgtgcg cgcgccggta tgcggcctcg
     1501 gcctccgccg catgcccgag ctgacgctcg aggttgccga gattcaactg tgcctcggcg
     1561 cggaaccggt cgaactccaa cgactcacgg tactccgcga tcgctcgatc gagcgccagc
     1621 cgtcggtcgg cgtccagccg gtcggccgcc gtgcccgcga gggacgcggc ggcggcgagc
     1681 cgcaccgtgc gaaccgggtc gtcgaggagc ccggcggcca gcgcggcgcc tgcatccgga
     1741 tcgatctcgc cggccagctc cgcgaccacg cggcgcacga gcggatccgc gtcggccgcc
     1801 gcgcgcccga gaaccgccgc caggtcgcgc accggtgcac gccacatctc ggcgagcgcc
     1861 gtcgcgcgga cgatcgccgg ctgcgccgga tcgccgacga cgcgccgcag cagcgccggt
     1921 ccgtcgggat ggccgatgcg ggccgcatgg agcgccgtgc cgtagtggaa cctgcccgag
     1981 cggccctcgg gaaaccagcc ggcgaccgtc gtcgccgccc agcgcggact ccgttccgca
     2041 tggcagtcgt tgcaggcgtt gctgacgccg agcgcgaccg acaggtcggg ccgcggcacg
     2101 cggaagccgt gatcgcgtcg cgggtcgatg atcatgaact tccgctccgg catgtggcag
     2161 tcgacgcagc gggtcccggc ccggccctgc gcgtgatggt gatgctccgg cgtatcgaac
     2221 gtcgccggcg cgtggcacgt actacacaac gcgttgccgt cgaggacgag ccgcgcggag
     2281 tgtggatcgt gacagtcgct gcaggtcacg ccttcggcgt gcatgcggct ctggaggaac
     2341 gagccgtact cgtagacctc gtcgaggatc tgaccgtccg gttggtacag gccttgttcg
     2401 agcagggcga cacggtgcgt atcggcgagc ggccggcccg ggaggtaatc ctcccacacc
     2461 caaccgcgtc gcgagtggca gcgggcgcac gtctcgagct cggcgtccga cgagcgcggc
     2521 accgaccggc gcgccgtcgg tcgcgcgccg tcgaagaccc agcggccgcc gtccgtgctc
     2581 cgcaggccgg cgaccagccc gcggcccgca tctcgcccgc gcgcggcggc gtcgcgctcg
     2641 acccagtcca cgtgcagcga cgccggaccg tgacacgcct cgcacgacac gtcgacctcg
     2701 gaccacgtgg tgtcgaagcg gtcctcggcc ggcgcgtaat tccgcgcgag cgcggtcgag
     2761 tggcactcgg cgcacatgta gttccagttc tgctgcggcc cggtccagtg caacggctcg
     2821 cgatggtcgc gggcgtcggc gggatacagg tggaaccagc gctgcccgcc ttcgccggcc
     2881 ggcctcgcgt cccacgccag accgagcgcc tggtagcgcc cgccgggcat ctcgatcagg
     2941 tactgctgga gcggggtcac gccgaaggtg taggccacgc ggaaggtggc cggcgcaccg
     3001 tcggggccgt cggtgcgtac ccggtactgc ccgtcctcgg tgaagaaggt cgagccgacc
     3061 ccgtgagccg cgaccgacgc gccgtcgaag tcgccgagca cggtctcgcc ggtcgccggc
     3121 tgcatcgcca ggtcgtggtg cgagccgcgc caggcctcga cctgctgccg atgacacggc
     3181 acgcaggcgg cgacgccgac gtagccgggc tcggccggcg cgacgggcgc gaccggcttc
     3241 ccgcaggcga cggcgccgat cgtcgaaacg aggagccagc aggcgcgccg cgtcatgccg
     3301 ccgggtggat catcgcacgt ccgaggtgcc ctcgatcatc ttctggatct tctccagatt
     3361 gatgtcgtcg ccccagcgac gaggcgtgac cgatacggct ttcgcgagtc tcatcggtct
     3421 cagctctcca gcgtggcttc catcgtgatc tcggcgtcga gcaaccggga gatcggacag
     3481 cccgccttcg cgccgtcggc cacctcgagg aacttcgccc gctcggcgcc cggcacccgt
     3541 gccctcaccg tcaggtggct gcgcgtcacc gtcggcttgc cgtcgaccgt ctcgagcgtc
     3601 acggcggcgg tcgtctcgat ccgcgtcggt gtcagaccgg ccttgcccag ctcggccgac
     3661 aacgccatcg agaagcaacc cgcgtgcgcc gcggcgatca gctcttccgg gttcgtcccc
     3721 ggcttgttct cgaaccgcgt ggcgaaggtg tagggggcgt cgtcgagcac gccgctc
//
LOCUS       DASPGN010000480         6863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1259331, whole genome shotgun sequence.
ACCESSION   DASPGN010000480 DASPGN010000000
VERSION     DASPGN010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259331"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2471
                     /locus_tag="VD788_13985"
     CDS             <1..2471
                     /locus_tag="VD788_13985"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV77423.1"
                     /translation="TLHDGREIPMTVRRDPPRDRAWEDLRQLRLATPAGVLPLEAIAD
                     IRRMPPAPAIVHHNGRREVKVAYTLAADVPTGGPARLALDAELRRAVQDVHRPAGYTI
                     ETPAPEASYSWFRRILVPVLLLLFAVLAVTFESLTMPLLVLLSLPLTLLGATWALVLS
                     GTPPDMMALVGALALIGLTVNPAILLVDRMQQRAWIGGRSSGAAALAAVRERARAVLM
                     TTATTVAGLWPLALVTGQENEIWPPFATIVMGGLVTSTLLTLLAIPVGFVFMRRFDEM
                     LGRLGPWIIIGWALATAAIVVPLFETEQIVSLTWQIVTTVLVASGLLLSIVFVFRRKE
                     TPEPDTSHGPPEVDVRYLSKIYGKPGPIGRAWHAGSRFAGRVQALGGKPFDPRVARDR
                     LIPLSIVLAGVLYLVVSVQTVFWSLFFLFVAAGLASALLIELRRARGGADELGRVLPG
                     GAEGVAAAAVPWIAALWAGISYHLLPRLAEERVKLAWWVWAVGALVLLVVQFGRRNAR
                     RIARGEIDERVERGKLRRTRTAWRKLSRRLFGLDLPAREIRAVTHVHFKATGGMIGVL
                     GPNGAGKTTLLRNLAGILEPSLGTITVGGVPLGRLRRYLARFVGYLPQDFGLPHDLTA
                     REYLEYYALHYEIRPDRRRERVERLLREVGLAERAGEKIGSYSGGMRQRVAVARTLLR
                     LPPVIIVDEPTVGLDPRERIRFRNLLSRLAEGRIVLFSTHVVEDVEVACERVIVMAHG
                     RIVFDGEPGALAGAAEGKVWVARLGPGEADADDLRALVVDQVPEADGTVRIRMLHDRP
                     PRAGAEPVAPSLEDGYLWLVRGTA"
     gene            2447..3205
                     /locus_tag="VD788_13990"
     CDS             2447..3205
                     /locus_tag="VD788_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77424.1"
                     /translation="MAGPGHGVSRVVTTTRRARRGPADAGRLLVLSLRMVAGRRFWVA
                     PLLPLIWPAFQILRLLLGWRPQGYTAEEAQNLLIAFPLVVLAIGLGVRIIAAEIDRRT
                     LEIAYTVPGGAHKVWLAKLAATSLLLLATELLLAAVAYAFCTAFPIVATLYGAWQPAM
                     FFLVLAMALSALFRSEAAGALVTAAVLALCWPLQQAGTRLSPFWNPAALTDADPSNLV
                     AWTVQNRLGFLLLALALVVLAFGRAENREKLLGG"
     gene            3394..4917
                     /locus_tag="VD788_13995"
     CDS             3394..4917
                     /locus_tag="VD788_13995"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXV77425.1"
                     /translation="MPIADLKIDLGSDVPVYRQIVDGVRAAAFEGELQPGHQLPPTRD
                     LARDLGVNRNTVVAAYEALADEGWVRSHTGRGTFLVRRPGASADSAVADSDAWFTAFS
                     RTADGAAAGGLQSILSLFVEPTGGVSFVGSYPAGELIPVDAFSEAMARVLAERGAEAL
                     GYGPTAGDPGLRETIAAGMRGQRMPAAADDILVTNGAQQGLEIVFRTFLDRGDAVVIE
                     EPTYTGALSVLGALGARVVGVPLDSQGIRADLLQMALERHRPRLVYLQPTFHNPTTRV
                     MGETRRREVLELAHRFRCPIVEDDWAGDLRLDGEDLPTLHALDSGRHVVYLSTFSKKL
                     MPGLRLGWVSAPPPVMQRLIEMKRVQDCGTSPLMQAALADFLGRGGLDDHLQRIRPAY
                     RRRRDAMLSALERHFPEEARWESPLGGMFVWIRLPEGFDGADLFVAARQEGVLYSRGE
                     LFHSDGSGGDTMRLTYSSASHEQIELGVATLGRLIGERRPAGHRPVRHRPAETMPIF"
     gene            4968..5867
                     /gene="pdxS"
                     /locus_tag="VD788_14000"
     CDS             4968..5867
                     /gene="pdxS"
                     /locus_tag="VD788_14000"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HXV77426.1"
                     /translation="MDDKRDVSKGTLRLKTGLAEMLKGGVIMDVTTADEAAIAQEAGA
                     VAVMALERVPADIRKEGGVARMCDPAKIEEIIGTVSIPVMAKCRIGHFAEARVLEALG
                     VDYIDESEVLTPADEEFHVDKLAFEVPFVCGCRNLGEALRRVGEGAAMLRTKGEPGTG
                     NIVEAVRHMRAVTSAIRRLTRLGPEEWMTESKNLGAPYELVRMVAADGKLPVPNFAAG
                     GIATPADAALMMLLGAESVFVGSGIFKSSDPPRMARAIVRATTHYNEPKLVAEVSRGL
                     GQAMRGLEIAGLAEAELMQHRGW"
     gene            5867..6526
                     /gene="pdxT"
                     /locus_tag="VD788_14005"
     CDS             5867..6526
                     /gene="pdxT"
                     /locus_tag="VD788_14005"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HXV77427.1"
                     /translation="MERIGILALQGDFARHAAAFAELGQATAEVRHVAELARIDGLVI
                     PGGESTTLLKLMEDEPWFDGIRRFHDDGGAVWGTCAGAILLAREVLDPCQPSVGLLDA
                     VIRRNAFGRQIDSFETELSGVGRFDRTPAVFIRAPRFVALGAQVDVLARLRDEPVLVR
                     QGRVLAATFHAELTADRRLQQAFIRMIGTHRQPTHEPPVPRTVSEPAHPSGAAERRIS
                     C"
     gene            6520..>6863
                     /locus_tag="VD788_14010"
     CDS             6520..>6863
                     /locus_tag="VD788_14010"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXV77428.1"
                     /translation="MLIYIDGKLIPKEEAKVSVFDHGLLYGDGVFEGIRVYDGNIFRL
                     KEHLERLFESAKTIGLQVPMTYEELETATVETVAANGKRDAYIRLVITRGVGDLGIDP
                     TTCKKATIFIIV"
BASE COUNT          957 a         2324 c         2466 g         1116 t
ORIGIN      
        1 tcactctcca cgacggacgc gagatcccga tgaccgtgcg gcgcgacccg ccgcgcgacc
       61 gggcctggga ggatctgcgg cagctccggt tggcgacccc ggccggcgtg ctgccgctcg
      121 aggcgatcgc cgacatccgc cgcatgccgc cggcgccggc gatcgtgcac cacaacggac
      181 gtcgcgaggt caaggtcgct tacacgctcg cggccgacgt gccgaccggt ggccccgcgc
      241 ggctcgcgct cgacgcggag ctgcgtcgtg cggtgcagga tgtccaccgc cccgccggct
      301 acacgatcga gaccccggct cccgaggcgt cgtacagctg gtttcgccgg atcttggtgc
      361 cggtgctgct gctgctgttc gccgtgttgg ccgtgacctt cgagtcgctg acgatgccgt
      421 tgctcgtgct gctgtcgctg ccgctgacgc tgctcggtgc gacctgggcg ctcgtcttga
      481 gcgggacgcc gccggacatg atggcgctcg tcggcgcgct ggccttgatc ggcttgaccg
      541 tcaatccggc gatcctgctc gtcgatcgga tgcagcagcg ggcgtggatc ggtggtcgtt
      601 cctcgggcgc cgcggcgctg gccgccgtcc gcgagcgtgc gcgggccgtg ttgatgacga
      661 cggcgacgac ggtcgcgggg ctgtggccgc tcgcgctcgt gaccggtcag gagaacgaga
      721 tctggccacc gttcgccacg atcgtcatgg gcggactcgt cacctcgacc ttgctgacgc
      781 tgctcgcgat ccccgtcggc ttcgtcttca tgcggcggtt cgacgagatg ctcggccggt
      841 tgggcccgtg gatcatcatc ggttgggcgc tggccaccgc ggcgatcgtc gtcccattgt
      901 tcgagacgga gcagatcgtc tcgctcacct ggcagatcgt gacgaccgtg ctggtcgcct
      961 ccggcctgct gctgtcgatc gtcttcgtct tccgccgcaa ggagactccg gagccggata
     1021 cgtcgcacgg acctcccgag gtcgacgtgc gctatctgtc caagatctac ggcaagccgg
     1081 gtccgatcgg ccgggcctgg cacgccggca gtcgcttcgc aggccgcgtg caggcgctcg
     1141 gcggcaagcc gttcgatccg cgcgtcgcgc gcgatcgtct gatcccgctc tcgatcgtgc
     1201 tggccggcgt gctctacctg gtcgtctcgg tgcagaccgt cttctggagt ctgtttttcc
     1261 tgttcgtcgc cgccgggttg gcctcggcgc tcttgatcga gctgcgccgg gcgcggggcg
     1321 gcgccgacga gctcggacgc gtgctgcccg gcggagcgga aggcgtcgcc gccgcggcgg
     1381 tgccgtggat cgcggcgctg tgggccggga tctcctacca cctgctgccg cggctggccg
     1441 aagagcgggt caagctggcc tggtgggtgt gggccgtcgg cgcgctggtg ctgctcgtcg
     1501 tccagttcgg ccgccgcaac gcgcgtcgca tcgcgcgcgg cgagatcgac gagcgggtcg
     1561 agcgcgggaa gctgcgccgg acgcgaacgg cgtggcgcaa gctcagccgc cggctgttcg
     1621 gtctcgacct gccggcccgc gagatccgcg cggtgaccca cgtgcacttc aaggcgaccg
     1681 gcgggatgat cggcgtgctg ggacccaacg gagcgggtaa gaccacgctg ttgcgcaacc
     1741 tggccggcat tctcgagccg tcgctcggca cgatcacggt cggcggcgtg ccgctcggtc
     1801 ggctgcggcg ctacctcgcg cgtttcgtcg gctatttacc gcaagacttc ggcttgcccc
     1861 acgacctcac cgcacgcgag tacctcgagt actacgcgct gcactacgag atccgtcccg
     1921 accgccggcg cgagcgcgtc gaacggctgt tgcgcgaggt cgggttggcc gaacgcgccg
     1981 gcgagaagat cggatcctac tcgggcggga tgcgtcagcg cgtcgccgtc gcccggacgc
     2041 tgctgcggct gccaccggtg atcatcgtcg acgagccgac ggtcggtctc gacccgcgag
     2101 agcgcatccg gttccgcaac ctgctgtcac ggctggccga gggccgcatc gtgctgttct
     2161 cgacgcacgt ggtcgaggac gtcgaggtcg cctgcgagcg ggtgatcgtc atggcccacg
     2221 ggcgaatcgt cttcgacggc gagccgggcg cgctggcggg tgccgccgag ggcaaggtct
     2281 gggtcgcgcg gctcggcccg ggtgaagcgg atgcggacga tctgcgcgcg ttggtcgtcg
     2341 accaagtgcc cgaggccgac gggacggtgc gcatccgcat gctgcacgac cggccgccgc
     2401 gggccggggc cgagccggtg gcaccgtcgc tcgaggacgg ctacctgtgg ctggtccggg
     2461 gcacggcgtg agccgggtcg tcaccacgac gcgccgcgct cgccgcggtc cggcggacgc
     2521 cggccgcctt ctcgtgttgt cgctgcgcat ggtcgccggc cgccggttct gggtcgcccc
     2581 gctgttgccg ctgatctggc ccgcgttcca gatcctccgc ctgctgctcg gctggcgccc
     2641 gcagggctac accgccgagg aggcgcagaa cctgttgatc gcgttcccgc tcgtcgtgct
     2701 cgcgatcggt ctcggcgtgc ggatcatcgc cgcggagatc gaccggcgaa cgctcgagat
     2761 cgcctacacc gtgcccggcg gcgcccacaa ggtgtggctc gccaagttgg cggcgacctc
     2821 gctgctgctg ctcgcgaccg agctgctgct cgccgccgtc gcgtacgcgt tttgcaccgc
     2881 gttcccgatc gtggcgacgc tgtacggcgc gtggcagccg gcgatgttct tcctcgtgct
     2941 ggcgatggcg ctgtcggcgc tgttccgcag cgaggcagcc ggggcgctcg tcaccgccgc
     3001 ggtgctcgcg ctgtgctggc cgctgcagca ggccgggacg cgtctgtcac cgttctggaa
     3061 ccccgccgcg ctgaccgacg ccgatccgtc caacctggtg gcctggaccg tacagaaccg
     3121 cctcggcttc ctgctgctcg cactggccct ggtcgtgctg gctttcgggc gcgccgaaaa
     3181 ccgcgagaag ctgctcggcg gctaaccgct cgccaaccaa acccgaccac gtgagtcgag
     3241 ggacgggaac ggtcgtcctc ggagaatcgt acggtataaa ttgttcatat gagaagcttt
     3301 atccgaacat accccgaaag aacacatacc ccttgaatcc gactctggat cgactagaat
     3361 ctcctattgg actaggccaa taggagacgc cggttgccca tcgccgactt gaagatcgac
     3421 ctcggcagcg acgttccggt ctaccgccag atcgtcgacg gggtccgggc ggcggcattc
     3481 gagggcgagc tgcagcccgg gcaccaactt ccgccgaccc gcgacctggc ccgcgatctc
     3541 ggggtcaacc ggaacaccgt cgtcgccgcg tacgaggccc tggccgacga gggttgggtc
     3601 cgcagccaca ccggtcgggg gacgtttctc gtccggcggc cgggcgcgag cgccgattcg
     3661 gccgtggccg actccgatgc ctggttcacc gcgttctcgc ggaccgccga cggggcggcg
     3721 gccggcggtc tccagtcgat cctgtcgttg ttcgtcgagc cgaccggggg cgtgtcgttc
     3781 gtcggctcct accccgccgg cgagctgatt cccgtcgacg ccttctccga ggcgatggcg
     3841 cgggtcctcg ccgagcgcgg ggcggaagcg ttgggctacg gaccgaccgc cggcgacccg
     3901 gggctgcgcg agacgatcgc ggccgggatg cggggccagc gcatgcccgc cgcggccgac
     3961 gacatcctcg tgaccaacgg cgcgcagcag gggctcgaga tcgtcttccg cacctttctc
     4021 gatcgcggcg acgcggtcgt gatcgaggag ccgacctaca ccggcgcgct gagcgtgctg
     4081 ggagcgctgg gcgcgagggt cgtcggcgta ccgctcgact cccaggggat ccgtgccgat
     4141 ctgctgcaga tggcgctcga gcggcaccgg ccgcggctgg tctatctaca accgaccttc
     4201 cacaacccga cgacacgcgt gatgggcgag acgcgacgcc gcgaggtgct cgagctggcc
     4261 caccgtttcc gctgtccgat cgtcgaggac gactgggccg gagacctgcg cctcgacggc
     4321 gaagacctgc cgacgttgca cgcgctcgac tcgggccggc acgtcgtcta cttgagtacc
     4381 ttctcgaaga agctgatgcc cggcttgcgt ttgggttggg tctcggcgcc tccgccggtc
     4441 atgcagcggc tgatcgagat gaagcgcgtg caggattgcg gcaccagccc gctgatgcag
     4501 gccgcgctcg cggatttcct cggtcgcggc ggtctcgacg atcacctgca gcgcatccgg
     4561 ccggcctacc ggcggcggcg ggacgcgatg ctctccgcac tcgagcgcca cttccccgag
     4621 gaggcgcgct gggagagtcc gctgggcggg atgttcgtct ggatccgcct gccggaaggc
     4681 ttcgacgggg ccgacctgtt cgtcgccgcc cggcaagagg gcgtgttgta cagccgcggc
     4741 gagctgttcc acagcgacgg ctccggcggc gacacgatgc gcctgaccta ctcgtcggca
     4801 tcccacgagc agatcgagct gggcgtcgcc acgttggggc gactgatcgg tgagcgcagg
     4861 ccggccggcc accggcccgt ccgtcaccgc ccggcggaga cgatgccgat cttctgattc
     4921 acccgaggcg agtgccgcgc aaggcggcag gaagaggcga gaagaccatg gatgacaaac
     4981 gggacgtcag caagggcacg ttgagactga agaccggtct ggccgagatg ctcaagggtg
     5041 gcgtcatcat ggacgtcacc acggccgacg aggcggcgat cgcccaggag gccggcgcgg
     5101 tcgcggtgat ggcgctcgaa cgggtgccgg ccgacatccg caaggagggc ggggtcgcgc
     5161 gcatgtgcga tccggccaag atcgaggaga tcatcggcac cgtgtcgatt ccggtgatgg
     5221 ccaagtgccg catcggacac ttcgccgagg cccgggtgct cgaggcgttg ggcgtcgact
     5281 acatcgacga gagcgaggtg ctgacgccgg ccgacgagga gttccacgtc gacaagctgg
     5341 cgttcgaggt cccgttcgtc tgcggttgcc gcaatctcgg cgaggcgctg cgccgagtcg
     5401 gtgagggtgc cgcgatgctg cgcaccaagg gcgagcccgg taccggcaac atcgtcgagg
     5461 ccgtgcgcca catgcgcgcc gtcacctcgg cgatccgccg gctgacgcgt ctcggaccgg
     5521 aagagtggat gaccgagtcg aagaacctcg gtgctccgta cgagctggtc cgcatggtcg
     5581 ccgccgacgg caagctgccg gtgccgaact tcgccgccgg cggcatcgcc acgccggcgg
     5641 acgcggcgct gatgatgctg ctcggcgccg agtcggtgtt cgtcggttcc ggcatcttca
     5701 agtcgagcga cccgccacgg atggcgcggg cgatcgtgcg ggcgacgacg cactacaacg
     5761 agccgaagct ggtcgccgaa gtgtcacgcg gcttgggcca ggcgatgcgc ggcctggaga
     5821 tcgccgggct ggccgaggcc gagttgatgc agcaccgggg gtggtgatgg agcggatcgg
     5881 catcctcgcg ctgcagggcg atttcgccag gcacgccgcg gcgttcgccg agctgggaca
     5941 ggcgaccgcc gaggtgcgac acgtcgcaga gctcgcgcga atcgacggcc tggtcatccc
     6001 gggcggtgag agcacgaccc tgctcaagct gatggaagac gagccgtggt tcgacgggat
     6061 ccgtcgcttt cacgacgacg gcggcgcggt ctggggcacc tgcgccggtg cgatcctgct
     6121 cgcacgagag gtgctcgatc cgtgccagcc gagcgtcggt ctgctcgatg cggtgatccg
     6181 gcgcaacgcg ttcggccgcc agatcgactc gttcgagacc gagctttctg gggtgggacg
     6241 attcgaccgt acccccgccg tcttcatccg cgcgccgcgg ttcgtcgcgc tcggcgcgca
     6301 ggtcgacgtg ctggcgcggc tccgcgacga gccggtgctg gtgcgacagg ggcgcgtgct
     6361 cgcggccacg ttccacgcgg aattgaccgc ggaccgtcga ttgcaacagg cgttcatccg
     6421 aatgatcggg acccacagac aaccaacgca cgaaccaccc gtcccccgaa ccgtgagtga
     6481 gccggctcac ccgtcgggag cggccgaacg gaggatctca tgctgattta catagacggc
     6541 aagctcattc cgaaggaaga ggcgaaggtg tcggtgttcg accacggctt gttgtacggg
     6601 gacggcgtgt tcgaggggat ccgggtctac gacggcaaca tcttccgcct gaaggagcac
     6661 ctcgagcgcc tgttcgagtc ggccaagacg atcggcctgc aggtcccgat gacctacgag
     6721 gagctggaga cggcgacggt cgaaacggtg gcggccaatg gcaagcgcga cgcctacatc
     6781 cgcctcgtga tcacgcgcgg ggtgggcgac ctcggcatcg atccgaccac gtgcaagaag
     6841 gccacgatct tcatcatcgt gga
//
LOCUS       DASPGN010000481         4151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1493925, whole genome shotgun sequence.
ACCESSION   DASPGN010000481 DASPGN010000000
VERSION     DASPGN010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493925"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /locus_tag="VD788_14015"
     CDS             <1..1582
                     /locus_tag="VD788_14015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77429.1"
                     /translation="YMRDVDDYEIRPIEGTADAASPFFSPDGEWIGFIDTREARLKKV
                     RADGGVPVTLCDSPNRAGHWGTDGRIYFSGVSDGIYAVSENGGAPEMLSEPVPEHGEK
                     TRRFPRVLPGGRGVLFTLGTTKMESYDEAAVALLDPETGEHRVLLEGGTKPSYLPTGH
                     ILYGRAGALYAIPFDLETLEVGGAPFLVADEVVTSDGWGEAQYAVSDNGTLVYVPGGP
                     EIYKFTVYWVDRDGRAEALPLTPRDYSQALFSPDGKKLEITSLGANATIWVYEIERTT
                     MQRLTTEWDHYAAIWTRAGGHVTVASGRAGQYGLWNVAADGSGTEELLLRTEDGGYPG
                     TWTPDDRALVFALSYPGTGLDILTLTLDGKPAVTPVIQTSFQESNPKLSPDGRWLAYS
                     SDAAGQTELYVQRYPGGGRRWQISAGGGEAPLWSPDGRELYFWKGGRMMAVSIETDGG
                     GFTPSKERQLPQPEFSALLDWDISPDGERFVVVGRLATVVTSDDPTVTNASPNRVGAQ
                     VPEIRVVTGWFEELRKKR"
     gene            1676..2083
                     /locus_tag="VD788_14020"
     CDS             1676..2083
                     /locus_tag="VD788_14020"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXV77430.1"
                     /translation="MAGDRSAIRRFLDGGPFAVAGASRSRTKYGNKVLRAYLQTGRRV
                     YPVNPNADDVEGLRAWPDLASLPERPHALSIVTPPDTTQAIVRDAIELGIEHLWLQPG
                     AEQDSAIGLAERAGCNIIHSGPCILVALSFREA"
     gene            complement(2094..>4151)
                     /locus_tag="VD788_14025"
     CDS             complement(2094..>4151)
                     /locus_tag="VD788_14025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV77431.1"
                     /translation="GERGTAGTLVPPLLVFLVALAVRLVAIHLGFAEQFFFGKYLVLG
                     RALRESGLIADRPFAYAPIYTYFVAIAQSVFGDALYPVLVVQAVIGSISCVLILRIAR
                     RLVGPGWALLPAGIACIHRSFVLYDTTFLSDSLGLALQLGLLAALMRLEPSSRWPVFV
                     LAGVLTGASVLHRPNNLLLLPLFAAVWCWISPWRAIALRQAAVVAGVALAVTLPVVVQ
                     NFRLSGTPGITASNPGYIFYSSNNGSSYGFRYSPPELFYRGSAYYERRTDRPFLGDAE
                     IATVISGAVVGRPMTLAESSRYYTVFTLRHMGRYPAHYASLWFQKLWLSVNGYESHDV
                     LPAFVRLDAIRSVTPIGLAWIAPLGLLGLGLSFRRPRGAVWLHLLLVNNLALLLAFYV
                     VVRFRLPLEAVLVILTGPALATLYRWLRQGAWGRSTAAAVALATLGLACNVSFPQAAQ
                     RARSRAMEVLLEDGERRMRRGDLAGARRQLERMICEDREELPRMLQAHRLLAEIYARP
                     GASFAGSAMRSPEHFDSGAVTARLAAKRRAGTITFEERRYLGHLYLEGGRADEARRVL
                     GEALASRRPEPLTRYELARALGALDDRAGAVRELERAIGDGLLFTSRGLHAALLLART
                     CRDRGDPAGARSWIEQASRQSALAPWEPDGEALVPLLAELRALPGYREPLPLDALIPP
                     VGR"
BASE COUNT          698 a         1464 c         1443 g          546 t
ORIGIN      
        1 gtacatgcgc gacgtcgacg actacgagat ccgaccgatc gaaggcacgg ccgatgccgc
       61 ctccccgttc ttctcccccg acggagagtg gatcggcttc atcgatacgc gcgaggcccg
      121 cctgaagaag gtccgcgcgg acggcggcgt cccggtgacg ctgtgcgact ccccgaaccg
      181 ggccgggcac tggggcaccg acggcaggat ctacttctcc ggcgtttccg acggcatcta
      241 tgcggtctcc gagaacgggg gcgcgcccga gatgctgtcc gagccggtgc ccgagcacgg
      301 cgagaaaacc cgccgctttc cccgcgtgct tcccggcggc cgcggcgtac tgttcacgct
      361 cggaacgacg aagatggaat cgtacgacga agccgccgtc gccctgctcg atcccgaaac
      421 cggcgagcac cgcgtgctgc tcgaaggggg caccaagccg tcctacctac ctaccggcca
      481 catcctgtac ggccgcgccg gcgcgctgta cgcgatcccg ttcgacctcg aaacgctcga
      541 ggtcggcgga gcgccgttcc tcgtggccga cgaggtcgtc acctcggacg gctggggcga
      601 ggcgcagtat gccgtctccg acaacggtac gctggtgtac gttccgggcg gacccgagat
      661 ctacaagttc accgtctact gggtcgaccg cgacggccgg gccgaagcgc tgccgctgac
      721 gccgcgggac tactcacagg cactcttctc gcccgacggc aagaagttgg agatcacctc
      781 gctcggcgcc aacgcgacga tctgggtcta cgagatcgaa cggaccacga tgcagcgcct
      841 gaccaccgag tgggaccact acgccgcgat ctggacgcga gccggcggac acgtgacggt
      901 cgcctccggc cgcgccggac aatacgggct ctggaacgtc gccgccgacg gaagcggtac
      961 ggaggagctt ctgctccgca cggaagacgg cgggtatccg ggtacgtgga cacccgacga
     1021 tcgggcgctc gtcttcgcac tatcgtaccc ggggaccggg ctcgacatcc tgaccctcac
     1081 gctggacggg aagcccgcgg tcacgccggt catccagacg tcgtttcagg aaagcaaccc
     1141 gaagctctcg cccgacgggc gctggctggc gtactcgtcg gacgccgccg ggcagaccga
     1201 gctgtacgtg cagcgctatc ccggaggcgg ccgtcgatgg cagatctccg ccggcggcgg
     1261 cgaggccccg ctgtggtcgc cggacggccg cgagctgtac ttctggaagg gcggtcgcat
     1321 gatggcggtc tcgatcgaga cggacggcgg cgggttcacg cccagcaagg agcgccagct
     1381 gccccagccg gaattcagcg cgctgctcga ctgggacatt tccccggacg gcgagcgctt
     1441 cgtcgtcgtc ggtcgcctgg ccaccgtcgt gacgtccgac gatccgacgg tgacgaacgc
     1501 ctcccccaat cgtgtcgggg ctcaggttcc ggagatccgc gtcgtcaccg gctggttcga
     1561 ggagctgcgc aagaaacgct gacgatgtgc gcgctcaaag tcgaagcgcc accacgcacc
     1621 tgagctattc ccgctcgttc gcaaccggat gcccgcgggc ggctatactg cgccggtggc
     1681 gggagatcgg tcggcgatcc ggcggttcct cgacggcggt ccgttcgccg tcgccggcgc
     1741 ctcgcgctcg cgcaccaagt acggaaacaa ggtgctgcgg gcctatctgc agaccggacg
     1801 gcgagtctac cccgtcaatc cgaacgcgga cgacgtcgag gggctgcgcg cctggcccga
     1861 cctggcgtcg ctccccgagc gaccccacgc gctgtcgatc gtcacgccgc ccgacacgac
     1921 gcaggcgatc gtgcgcgacg cgatcgaact cggtatcgag cacctctggt tgcagccggg
     1981 cgccgagcag gactcggcca tcggcttggc cgagcgcgcg gggtgcaaca tcatccattc
     2041 gggcccgtgc atcctcgtcg cgctgtcgtt tcgtgaggcg taggtcgtgc gggtcagcga
     2101 ccgacgggcg ggatgagcgc gtcgagcggt agcggctcgc gatagccggg gagcgcgcgg
     2161 agctcggcca gcagcggcac cagcgcttct ccgtccggct cccagggcgc gagcgccgac
     2221 tgccgcgacg cctgctcgat ccaggaccgc gccccggccg gatcgccccg gtctcggcaa
     2281 gtccgggcga gaagcaaggc cgcgtgcaat ccccggctgg tgaacagcag tccgtcaccg
     2341 atcgcccgct cgagctcgcg gacggcaccc gcccggtcgt cgagcgcccc cagcgcgcga
     2401 gccagctcgt agcgggtcag cggctcgggc cgccgcgacg cgagtgcctc gcccaacacg
     2461 cgccgcgcct cgtccgcccg cccgccctcg aggtagaggt gaccgaggta gcgtcgctct
     2521 tcgaacgtga tcgtgccggc gcggcgcttg gccgcgagtc gcgcggtgac cgcgccgctg
     2581 tcgaagtgct cgggcgaccg catcgcgctg ccggcgaacg acgcgccggg tcgagcgtag
     2641 atctccgcca gcaggcgatg cgcctgcagc atccggggca gctcctcgcg atcctcgcag
     2701 atcatccgtt cgagctgacg tctggcgccg gcgagatcgc cgcgacgcat ccgacgctcc
     2761 ccgtcctcga gcagcacctc catcgcacgg ctgcgcgccc gttgtgcggc ctgcggaaag
     2821 ctgacgttgc aggccagacc gagcgtcgcc agcgcgacgg ccgccgcggt cgagcggccc
     2881 cacgcgccct gccgcagcca gcgatacagc gtggcgagcg ccggaccggt caggatgacc
     2941 agcaccgcct cgagcggcag gcggaaccgc acgacgacgt agaacgcgag cagcagcgcc
     3001 aggttgttga ccagcagcag gtggagccag acggccccgc ggggccggcg gaacgacaga
     3061 cccagaccga gcaggccgag cggggcgatc caggccaagc cgatcggcgt gacggaccgg
     3121 atcgcgtcga gccgcacgaa cgccggcagg acgtcgtgcg actcgtagcc gttgaccgac
     3181 aaccacagct tctggaacca cagcgaggcg taatgcgccg ggtagcgccc catgtggcgc
     3241 aaggtgaaaa ccgtgtagta gcgcgacgat tcggcgagag tcatcgggcg cccgacgacc
     3301 gcgcccgaga tcaccgtcgc gatctccgcg tcgccgagga acggccggtc ggtccggcgc
     3361 tcgtagtagg cgctgccgcg gtagaacagc tcgggtggac tgtaacggaa gccgtaggac
     3421 gacccgttgt tgctgctgta gaagatgtag cccgggttgc tcgcggtgat acccggtgtt
     3481 ccgctgaggc ggaagttctg cacgacgacc ggcagcgtca ccgcgagcgc cacgccggcg
     3541 acgaccgccg cttgccgcag cgcgatcgcg cgccacggcg agatccagca ccagacggcg
     3601 gcgaacaacg gcagcagcag caggttgttc ggccggtgca gtacgctggc accggtcagg
     3661 acccccgcca gcacgaacac cggccagcgc gacgacggtt cgagtcgcat cagcgccgcc
     3721 agcagaccga gctgcagggc cagacccagc gaatcggaca ggaaggtcgt gtcgtacagg
     3781 acgaagctgc ggtggatgca agcgattccc gccggcagca gcgcccagcc gggcccgacc
     3841 agccgccggg cgatccgcag gatcagcacg cagctgatcg agccgatcac cgcctgcacg
     3901 accagcaccg gatacagcgc gtcgccgaag accgactggg cgatggcgac gaagtaggtg
     3961 tagatcggcg cgtaggcgaa cggccggtcg gcgatcaggc cgctctcgcg taacgcccga
     4021 cccagcacga ggtacttgcc gaaaaagaac tgttcggcga atccgaggtg gatcgcgacg
     4081 agccgcacgg cgagcgcgac gaggaagacg agcagcggcg gcacgagcgt gccggccgta
     4141 ccgcgctccc c
//
LOCUS       DASPGN010000482         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_441244, whole genome shotgun sequence.
ACCESSION   DASPGN010000482 DASPGN010000000
VERSION     DASPGN010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441244"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VD788_14030"
     CDS             <1..266
                     /locus_tag="VD788_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77432.1"
                     /translation="GLAATIVGSELVGPREIEARYGLTGGQIYHGEHALDQLLVRPIP
                     ECLRHATPLDGLFLCGGGSHPGGGLTLAPGRLAADAIGAAVRS"
     gene            complement(256..762)
                     /locus_tag="VD788_14035"
     CDS             complement(256..762)
                     /locus_tag="VD788_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77433.1"
                     /translation="MRAARPLSVLIALLAAGGVAAEHGDHGAHAQKGDHEVALFLGVT
                     DDRGMDAFTWGLEYPYQFTDRVAGGAFFDKTEGDLRMSVLGGAFYYKPLPRLALMIGP
                     AVERLEPMPDGGHGENAETETELLLRVGVLYGFPVAARVSILPALYADFVDGETVWVG
                     GADVAFSF"
     gene            889..2052
                     /locus_tag="VD788_14040"
     CDS             889..2052
                     /locus_tag="VD788_14040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXV77434.1"
                     /translation="MRTTAECVVVGGGVVGLSIAYHVAARGMTDVVVLERERMVGTGS
                     TGRCAGGFRHQFSNEANVRLSLLSIDKLKRFAVEMDQPLDLHQDGYLFLLCEPAQVAT
                     FEAQVELQRRLGVAARMLRPEEIAAVLPGTELGIDDVLAASYCPDDGVSDPAGVTEGY
                     RRNATRLGVRIETGCEVTGVDVARGRVTGVDTSDGRRIAAPCVVNAAGPHAAAVGRMA
                     GVDVPVEPARRFIWTTHPFAGAPLRFTLVIDFSTGFYFHRESGGVLFGMGNRAEPPSF
                     SLDVDEEFFESVVIEVGMRRLPALADAGIRAAWAGSYEMSPDANPILGRVAGVDGFVL
                     ANGFSGHGFQHAPGVGQLIAELITDGAATSADLSPFSIDRFDGRALRAELGVV"
     gene            complement(2065..>2841)
                     /locus_tag="VD788_14045"
     CDS             complement(2065..>2841)
                     /locus_tag="VD788_14045"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXV77435.1"
                     /translation="TLARLGRTAAFAGVVGDDAAGRMIADGLRRERVGVDGLVRRAGF
                     RSPVSVILVENGRRAIIEHAQYGLPLAFDELERAGVGFDDCRTLLIDARLVAVQLEAA
                     RRVRRAGGHVVLDCGHPRPGVDELLSLTDVAIFSYTYAEAMLGASYDARVFLEAVAGR
                     LPPDGPRIAGLTLGPAGCAIYAPQSGFARIGGHAVDAVDPTGAGDVFHGAFVHAHLAG
                     RAPEQAARFANAAAALKCTAMTGRAVLPDEQAIWQLALGR"
BASE COUNT          391 a         1020 c         1044 g          386 t
ORIGIN      
        1 cgggtctggc cgcgacgatc gtcggctccg agctcgtcgg gccgcgcgag atcgaggcgc
       61 gctacggtct gaccggcggc cagatctacc acggcgagca cgcgctcgat caactgctcg
      121 tgcgcccgat cccggagtgc ctgcggcacg cgacgccgct cgacggcttg ttcctctgcg
      181 gcgggggatc gcacccgggc ggcggtctga cgctggcccc gggtcgcctc gccgccgacg
      241 cgatcggcgc cgccgtcaga agctgaaggc gacgtcggcg ccgccgaccc agacggtctc
      301 gccgtcgacg aagtcggcgt agagcgccgg caggatcgaa acgcgcgcgg cgaccggaaa
      361 gccgtagagc accccgaccc ggagcaggag ctcggtctcc gtttccgcgt tctcgccgtg
      421 cccgccgtcc ggcatcggct cgagccgctc gacggccgga ccgatcatca gcgccagccg
      481 cggcagcggc ttgtagtaga acgcgccgcc gaggacgctc atgcgcaagt cgccctcggt
      541 cttgtcgaag aacgcgccgc ccgccacgcg gtcggtgaac tggtaggggt actcgagacc
      601 ccaggtgaag gcgtccatgc cgcggtcgtc ggtcacgccg aggaacaacg cgacctcgtg
      661 gtcccccttc tgcgcgtgcg ccccgtgatc gccgtgctcg gccgcgacgc cgcccgccgc
      721 cagcagggcg atcagtaccg aaagtggccg cgccgctctc atcgttctcc tgcgtgcgag
      781 gagagcggag tataggcacg gccggttcgc ggttcagtgg ggtcaatgga cccaaaacgt
      841 ctcgcccgcg agcttgcggt gcaataatcc tggcgaagga gcgggcggat gaggacaacc
      901 gcggagtgcg tcgtcgtcgg cggcggtgtg gtcggtctga gcatcgccta ccacgtcgcc
      961 gcgcgcggca tgaccgacgt cgtcgtgctc gagcgcgagc ggatggtcgg caccggctcg
     1021 accggtcgct gcgccggcgg tttccgccat cagttctcga acgaagcgaa cgtccgcttg
     1081 tcgctgctca gcatcgacaa gctgaagcgg ttcgccgtcg agatggacca accgctcgac
     1141 ctgcatcagg acggctacct gttcctgctg tgcgaacccg cgcaggtggc gacgttcgag
     1201 gcgcaagtcg aactgcagcg gcggctcggc gtcgcggcgc gcatgctgcg accggaggag
     1261 atcgccgcgg tgctgcccgg caccgagctc ggcatcgacg acgtgctggc cgcgagctac
     1321 tgccccgacg acggcgtgtc ggacccggcc ggcgtgaccg agggttatcg ccggaacgcg
     1381 acgcggctcg gcgtccgcat cgagaccggc tgcgaggtga ccggcgtcga cgtggcgcgc
     1441 ggtcgcgtga ccggcgtcga tacgagcgac ggccggcgga tcgcggcgcc gtgcgtcgtc
     1501 aacgccgccg gcccgcatgc ggccgcggtc ggtcgcatgg ccggcgtcga cgtgccggtc
     1561 gagcccgcgc gtcgcttcat ctggacgacc cacccgttcg ccggcgcgcc gttgcgcttt
     1621 accctcgtca tcgacttctc gaccgggttc tacttccatc gcgagagcgg cggcgtgctg
     1681 ttcggcatgg gcaaccgtgc cgagccgccg agcttcagcc tcgacgtcga cgaggagttc
     1741 ttcgaatcgg tggtgatcga ggtcgggatg cggcgcctgc cggcgctggc cgacgcgggg
     1801 atccgggcgg cgtgggccgg cagctacgag atgtcgcccg acgccaaccc gatcctcggt
     1861 cgcgtggcgg gtgtcgacgg cttcgtgctg gccaacggct tcagcggtca cggcttccaa
     1921 cacgcgcccg gcgtcgggca gctgatcgcg gagctgatca cggacggcgc ggcgacgagc
     1981 gccgacctgt cgccgttctc gatcgaccgc ttcgacggcc gcgcactgcg ggccgagctg
     2041 ggcgtcgtct agcgcgcccg cgggctaccg accgagcgcg agctgccaga tcgcctgctc
     2101 gtcgggcagg acggcgcggc cggtcatcgc cgtgcacttg agcgccgcgg cagcgttggc
     2161 gaaacgcgcc gcctgctcgg gcgcgcggcc ggcgaggtgc gcgtggacga acgcgccgtg
     2221 aaagacgtct cccgcgccgg tcgggtcgac cgcgtcgacc gcatgcccgc cgatccgcgc
     2281 gaacccgctc tgcggcgcgt agatcgcgca gccggcggga ccgagcgtca ggccggcgat
     2341 gcgcgggccg tccggcggca gccggccggc gaccgcctcg aggaagacgc gcgcgtcgta
     2401 ggacgcgccg agcatcgcct cggcgtaggt gtaggagaag atcgcgacgt cggtcagcga
     2461 cagcagctcg tcgacccccg ggcgcggatg accgcagtcg agcacgacgt ggccgcccgc
     2521 gcgccgcacg cgccgcgccg cctcgagctg gaccgcgacg agccgcgcgt cgatcaagag
     2581 cgtgcggcag tcgtcgaagc cgacgccggc ccgctcgagc tcgtcgaacg cgagcggcag
     2641 cccgtactgc gcgtgctcga tgatcgcgcg ccggccgttc tcgacgagga tcacgctgac
     2701 cggcgagcgg aaaccggcgc gccgcaccag gccgtcgacg ccgacccgtt cccggcgcag
     2761 tccgtcggcg atcatccgcc cggccgcgtc gtcgccgacg acgccggcga aggcggccgt
     2821 acggcccagc cgggcgagcg t
//
LOCUS       DASPGN010000483        12663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_792487, whole genome shotgun sequence.
ACCESSION   DASPGN010000483 DASPGN010000000
VERSION     DASPGN010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12663
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792487"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1878
                     /locus_tag="VD788_14050"
     CDS             10..1878
                     /locus_tag="VD788_14050"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HXV77436.1"
                     /translation="MTDRCPRPGTRTAAAERGSALVLALLVTVILALLGISFLLLAET
                     ENRIAQNEKRAAQALYVAEAGLIAVKHWFDATGSAIGFPESSVVDRTLRVTIDETDPY
                     DAAAAQPADGIVGSRPYYKQAVDLDGDGGDDLFDKPYRGDDRHRFLGTPDGPDLRIDD
                     GDAAGRTFLDTLSERLFGGFPGEPGGVVARLSRIDLYAPPYERVGPGWRRYGLATVKV
                     TARLYRDPGNEREVLAEHQIEGVVSEIPYQGPHAPLHSCGPATFVPQASGEMTVRWGA
                     LSAVGDVHTADPIAGSAVPMSVPRTLPFGPGADQLFPVDETLFDLFKSTVVLDLPIQD
                     PWFRIVAGGQIEAFGGAATQPVPPSTTANQDHSNLMQNLGVVMCPRYDYDLWKNIATS
                     GGRNVRYFAYDSASGGFRENGTGNSETLQVLTDGQEGIFFFDTRDGHPPTDADADGVF
                     DNLTPAMVLSGAWDFSGFLYVNATKFEVSDVSGQAREIRPPGEPYLDLNENGKYDAGE
                     RYVNLDYATATAASATVRADDSSPGSAVRDARGPAIPDVPVSFRGILYTTGRFESVGE
                     GTFYGTVVAGQWVSQSPSDGSVPTPTLIWDESIVSGWPPEGSALPRVLITEWDSGG"
     gene            1961..2596
                     /locus_tag="VD788_14055"
     CDS             1961..2596
                     /locus_tag="VD788_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77437.1"
                     /translation="MTKSWLERASLSILIALFVGGVSLAEGEKKKTEPKGDSKTTTPT
                     AKKQPEPSPYTEATRKPSRAAGEGETVVITNEYLEQMMSGLSEEEKMRGVYQAERHIG
                     DPLPGDTPADETTPPSEGAAAADATTETSTPADAPKSVEELNAQVVELERRLLALKNP
                     LLPRRYSDKKDPEAKDWDEQSHQQKIATTEKELAAAKAALAEAQKGAGEPR"
     gene            2723..3661
                     /gene="argF"
                     /locus_tag="VD788_14060"
     CDS             2723..3661
                     /gene="argF"
                     /locus_tag="VD788_14060"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXV77438.1"
                     /translation="MRGASLAGRDLISLLDYTTEELERLLELAAEVKANPDHYAKRLT
                     GRTLFMYFEKPSLRTRVTFEAGMTQLGGHAIYYTAAEGKIGVRESVEDVARNLERWVD
                     AAMCRTFSHELLLELARFAEIPVINGLTDHLHPCQALADYMTLREIGGDSLAGRRLAY
                     VGDGNNVAHSLMNGGARLGVSVTVITPPGYEPDAGVVETARAAAQQSGATIEITHDIE
                     AVRGANAVYTDVWASMGQEDEAAERKRVFAPYQIHRGVMDLAGPETAFMHCLPAHRGE
                     EVTSEVADMPRSVIFEQAENRLHAQKAVLLALLGGV"
     gene            complement(3658..4422)
                     /gene="ubiE"
                     /locus_tag="VD788_14065"
     CDS             complement(3658..4422)
                     /gene="ubiE"
                     /locus_tag="VD788_14065"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HXV77439.1"
                     /translation="MTTRRPCKRECHRVNPDEPRGPAEVRSMFDRIARRYDLLNHLLS
                     ANLDHAWRRRAAAELAGAPAGEVLDLCGGTGDMSLAVARRSEVERVICCDFSHEMLRR
                     AEVKFRRRRPGSKCVTLEADGLRLPFPDERFGAVTVAFGVRNFDDRVRALREIGRVLR
                     PGGRLVVLEFSRPAGPWLGPVYRLYLKRVLPRLGGGIAGASGPYRYLAQTIAAFPDPP
                     ELAGRIRDAGFAACGWTTLSGGIVAVHTAIKGGPRA"
     gene            4569..5018
                     /gene="rplM"
                     /locus_tag="VD788_14070"
     CDS             4569..5018
                     /gene="rplM"
                     /locus_tag="VD788_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HXV77440.1"
                     /translation="MGTFVPKRGEIRRDWWLVDADGLTLGRLATEVASRLRGKHKPTF
                     TPYLDTGDHVIVVNAEKVVLTGRKLATKTYRRHSGYPGGLHEATARRLLATHPQRVIE
                     SAVKGMLPKGPLGRQMARKLKVYAGPSHPHVSQQPNKMELADAARNR"
     gene            5042..5446
                     /gene="rpsI"
                     /locus_tag="VD788_14075"
     CDS             5042..5446
                     /gene="rpsI"
                     /locus_tag="VD788_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXV77441.1"
                     /translation="MGVSEGTLQYYGTGRRKRAAARVYLRPGSGKFVVNDRAFEDYFP
                     SEMLKMVIRQPLVVTETSDKFDVLVRVVGGGCTGQAGALRHGISRALVNYNPELRRKL
                     KEAGFLTRDPRKVERKKYGQPGARKRFQFSKR"
     gene            5491..6381
                     /gene="rpsB"
                     /locus_tag="VD788_14080"
     CDS             5491..6381
                     /gene="rpsB"
                     /locus_tag="VD788_14080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXV77442.1"
                     /translation="MVTVTMKEMLEAGAHFGHQTRRWNPRMKRFIFGARNGIYIIDLK
                     KTSRHLREALAFVSDLSARGGTVLFVGTKRQAQEVVAEEAIRSGMYFIDQRWLGGTLT
                     NFTTIRKSIARLAELEELTSNDKAEALTKKERAKLDKERGKLQKTLSGIKTMSKLPQA
                     VFVIDPQKERIAVAEANKLGIPVVAVVDTNCDPQPIDYPIPGNDDAIRAIKLFAGRFA
                     DAIIEGRQIWESARRQTETETEQQVAAQPKSIADRVRAREARRERVRQQVQAKRVRRS
                     PSDGSGEVVAAGVDKNANSD"
     gene            6493..7092
                     /gene="tsf"
                     /locus_tag="VD788_14085"
     CDS             6493..7092
                     /gene="tsf"
                     /locus_tag="VD788_14085"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXV77443.1"
                     /translation="MQVTAAMVKELREKTGVGMMKCKAALSEANGDLSEAEKILRKQG
                     VAAAAKKADRATGEGLVEAYIHTGGKIGVMVEVNCETDFAARSDDFRELVRGIAMHVA
                     AAAPRFARREDVPQDVLETELEIAREQALRSGKPPAVVDKIVEGKIDRFYAENCLIEQ
                     PYVRDPDRTVGQLIHDAVSKIGENIQLRRFVRYVLGEAG"
     gene            7150..7869
                     /gene="pyrH"
                     /locus_tag="VD788_14090"
     CDS             7150..7869
                     /gene="pyrH"
                     /locus_tag="VD788_14090"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXV77444.1"
                     /translation="MEPASAPRYRRVLLKLSGEALIGDQSSGISPVIVAELAEEIVRV
                     HQLGVELALVVGGGNFIRGAQVTGIDRVTADHMGMLATLINSLALQDAIERHGLMTRL
                     LSALEIRQVAEPFVLRRALRHLEKGRVVIFAAGTGNPYFTTDSAAALRALEIKAEVLL
                     KATKVDGVYTADPATDPGAKKLDKIGYLETLERGLKVMDTTAISLCMDNQLPIVVFNV
                     RDPGNVTRIVRGEAVGSVVSE"
     gene            7875..8438
                     /gene="frr"
                     /locus_tag="VD788_14095"
     CDS             7875..8438
                     /gene="frr"
                     /locus_tag="VD788_14095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXV77445.1"
                     /translation="MDEARVLETAGERMDASVADAKQKLGTVRTGRASLAMLDGIRVD
                     YYGTPTPLNQIAKLSVPEPTLIVAQPFDPSTLTLIEKAIMASDLGLNPSNDGKIVRIP
                     IPPLTEERRKQLVKRVRALGEEAKTAIRQIRRDANEEIKKLEKDSAISEDAARRHLDE
                     IQKTTDRHTDKIDGLCKDKEKELMEIA"
     gene            8438..9643
                     /gene="ispD"
                     /locus_tag="VD788_14100"
     CDS             8438..9643
                     /gene="ispD"
                     /locus_tag="VD788_14100"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR00453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HXV77446.1"
                     /translation="MRPGARAATEFAGVVVAAGLATRFGGSTPKQFLELGGRALVERA
                     VSAIAAHPAVDRLVLVLSADRLDDPYVSRLRARHRGLAVVRGGATRADSVAAGLAAVE
                     ARFVLVHDAARPLASFAVVDRVVEATRRFGAAVPAIGVADTVKRVAPPTEPGGAMAVV
                     ETIDRSPLRLAQTPQGARLDWLRAALERARAEGAPVTDESSALERAGHRVAVVEGDVE
                     NRKITAAADLADARRRLTPAADGLRVGSGFDIHRVGEGRPLVLGGIGFPGEVGLTGHS
                     DADVVLHAVMDALLGAAGLGDIGLLFPPDDPAWAGADSRDLACVVARRIDEAGFAVVN
                     LDVTLLAERPKIAPRSVEMRDAIGRSLGIPSDRVGLKATTLERLGALGRGEGIACHAV
                     ALLVRRDER"
     gene            9640..11160
                     /gene="gltX"
                     /locus_tag="VD788_14105"
     CDS             9640..11160
                     /gene="gltX"
                     /locus_tag="VD788_14105"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXV77447.1"
                     /translation="MSGEPEAPRVRFAPSPTGSLHLGNARTALFNWLVARQGGGVFVL
                     RVEDTDIDRERAGSERAILEDLRWLGLDWDEGPDVDGPYGPYRQSERAQSYAAAARLL
                     LDRGRAYRCFCDERRLEEERERRVAEGRAPHYDGRCRRIGPAESSARSEREAFAVRFL
                     AAPADGADASERVTFVDRLRGGVEFPLAEIGDTVIVRRDGRPTYNFAVVVDDIQMKID
                     LVLRGDDHLSNTPRQVLIFEALGHPPPEFVHLPMVRGPDGTRLSKRHGAVSVGEYRRA
                     GYPVDGMINGLALLGWSPGDDRVVIDRSELVEQFRLDRIVRSPATFDPQKLEWICAQH
                     IQRMDESALLEPLRERLAEAGRLPADWSPALRAWAPALVPIVRTFISRFDQIPERCAP
                     VFHPGGRPPAADAETALVQPVAVEVVRALCELASVRPPIDLEAWHALRDGVRERAGVR
                     GKALFRPLRVALSGSESGPELDLLVPLIETGHAAAPDRIDAVATRARRTLEWLGAA"
     gene            11142..11927
                     /locus_tag="VD788_14110"
     CDS             11142..11927
                     /locus_tag="VD788_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXV77448.1"
                     /translation="MARRRLNGGEDDAAAFVYGVRPVGELLASRPHQIERIFVARDAR
                     GPVGRILREARHAGIPVSHVTRERLALRLGRRTESQGIAAVVAPRAYADSEEICRRAA
                     AAAAGLLVLVDRMTDPGNLGSLLRTCAAAGVDGVLLSREGTVGLTAHVLKASAGAAER
                     VPVGRETRPPNRVAELRKRGFEVLALDPRGEVAWDELDLRGPTMIVAGGEARGPRPAV
                     ADACSRKVAIPLAAGVESLNVAVAVAVLLFEALRQRRVACGDS"
     gene            11968..12043
                     /locus_tag="VD788_14115"
     tRNA            11968..12043
                     /locus_tag="VD788_14115"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12001..12003,aa:Thr,seq:tgt)
     gene            12172..12258
                     /locus_tag="VD788_14120"
     tRNA            12172..12258
                     /locus_tag="VD788_14120"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12206..12208,aa:Tyr,seq:gta)
     gene            12309..12384
                     /locus_tag="VD788_14125"
     tRNA            12309..12384
                     /locus_tag="VD788_14125"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12342..12344,aa:Gly,seq:tcc)
     gene            12422..12497
                     /locus_tag="VD788_14130"
     tRNA            12422..12497
                     /locus_tag="VD788_14130"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12455..12457,aa:Thr,seq:ggt)
     gene            12579..>12663
                     /locus_tag="VD788_14135"
     CDS             12579..>12663
                     /locus_tag="VD788_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXV77449.1"
                     /translation="MAKEKFDRSKPHVNVGTIGHVDHGKTTL"
BASE COUNT         2092 a         4193 c         4536 g         1842 t
ORIGIN      
        1 gcctgaccga tgactgatcg gtgcccgcgg cccggcacgc ggaccgcggc cgccgaacgc
       61 ggttcggcgc tggtgctcgc cttgctcgtc acggtcatcc tggcgctgtt gggcatctcg
      121 tttctgctcc tggccgagac cgagaatcgc atcgcgcaga acgagaagcg agcggcgcag
      181 gcgctgtacg tcgcggaagc cgggttgatc gcggtcaagc actggttcga cgcgacgggc
      241 tcggcgatcg gctttccgga gagctcggtg gtcgatcgca cgctgcgcgt gacgatcgac
      301 gaaaccgatc cgtacgacgc ggcggcggcg cagcccgccg acggcatcgt cggctcgagg
      361 ccgtactaca agcaggcggt cgatctcgac ggcgacggcg gcgacgacct gttcgacaag
      421 ccgtatcggg gtgacgaccg gcaccggttc ctcggcaccc ccgacgggcc ggatctgcga
      481 atcgacgacg gcgacgccgc cggccggacg tttctcgaca cgctctccga gcggctgttc
      541 ggcggcttcc cgggcgagcc cggtggcgtg gtcgcgcgcc tcagtcgcat cgatctctat
      601 gcgccgccct acgaacgggt cggacccggg tggcggcggt acggtctggc gaccgtcaag
      661 gtcacggcgc gactgtatcg cgaccccggc aacgaacggg aagtgttggc cgagcaccag
      721 atcgagggcg tcgtcagcga gatcccgtac caggggccgc acgccccgct ccattcgtgc
      781 ggacccgcca ccttcgtccc gcaggccagc ggcgagatga cggtgcgctg gggggcgttg
      841 tcggcggtcg gcgacgttca cacggcagac ccgatcgccg gcagcgcggt accgatgtcg
      901 gtcccgcgca cgctgccgtt cggccccggc gccgaccagc tctttcccgt cgacgagacg
      961 ctgttcgacc tcttcaagtc gaccgtggtg ctcgaccttc ccattcaaga tccgtggttc
     1021 cgaatcgtgg cgggcggcca gatcgaggcc ttcggaggcg cggcgacaca gccggtcccc
     1081 ccgtcgacga cggcgaacca ggaccactcg aacctgatgc agaacctggg agtcgtgatg
     1141 tgcccgcgct acgattacga cctctggaag aacatcgcga cgagcggtgg ccgaaacgtg
     1201 cgctacttcg cctacgactc ggcttcgggc gggtttcgcg agaacggcac cggcaactcc
     1261 gagacgctgc aggtgctcac cgacggacag gaggggatct tcttcttcga tacgcgagac
     1321 ggccaccccc cgaccgacgc cgacgctgac ggcgtcttcg acaacctgac accggcgatg
     1381 gtgctgagcg gcgcgtggga tttcagcggc ttcctctacg tcaacgcgac caagttcgag
     1441 gtctcggacg tcagcggaca ggcgcgggag atccgcccgc ccggcgagcc ctacctcgac
     1501 ctcaacgaga acggcaagta cgacgccggc gagcgctacg tcaacctcga ctatgcgacg
     1561 gcgaccgcgg cgagcgccac cgtgcgggcc gatgacagct cgccgggcag cgccgtgcgc
     1621 gatgcacgcg ggccggcgat ccccgacgtc ccggtctcgt ttcgcggaat cctctacacc
     1681 accggtcggt tcgaatcggt gggcgagggc acgttctacg gaaccgtcgt cgccggccaa
     1741 tgggtctcgc aatcgccgtc ggacgggtcg gttccgacgc cgaccttgat ctgggacgaa
     1801 tcgattgtca gcgggtggcc gcccgagggg tcggccctgc cccgggtact gatcacggag
     1861 tgggattccg ggggatgagc ggcccgggcg attggcgggt gacacgcggc ccgacaggca
     1921 ctaaattgtt ttcggggccg gtcccttgag gtgtgatgcg atgaccaaat cgtggctaga
     1981 acgagcttcc ctgtcgatcc tgatcgcgct gttcgtgggc ggggtgagcc tcgccgaggg
     2041 cgagaagaag aagacggagc ccaagggcga ttcgaagacg acgacgccga ccgcgaagaa
     2101 gcaacccgag ccgtcgcctt acaccgaggc cacgcgcaaa ccgtcccgtg ccgccggaga
     2161 aggcgagacc gtcgtcatca ccaacgagta cctcgagcag atgatgtccg gcctgagcga
     2221 ggaggagaag atgcggggcg tctaccaggc cgagcggcac atcggcgatc cgctacccgg
     2281 cgacacgccg gcggacgaga ccacaccgcc gagcgaaggt gccgccgcgg ccgacgccac
     2341 caccgagacc tcgacgcccg ccgatgcccc gaaatcggtc gaagagttga atgcgcaggt
     2401 cgtcgagctc gaacggcgcc tgttggcact gaagaacccc ttgcttcccc gtcggtactc
     2461 cgacaagaaa gacccggagg ccaaggactg ggacgagcag agccaccagc agaagatcgc
     2521 caccaccgag aaagagctgg ccgcggcgaa ggccgcgttg gccgaagcgc agaagggcgc
     2581 cggcgagccg cgctagccgg acgcgaccgt cacgacggcg cggggcgggc gatcgcttga
     2641 cagggtccga acccgctcgc tagagtgcgc ccgcggcgcg cgggaggctc gtcctcgacc
     2701 ggcgccgccc cgggaggata cggtgagggg cgcgagcttg gccggacgcg atctgatctc
     2761 gttgctcgac tacacgacgg aggagctcga gcgcctgctc gagctcgcgg ccgaggtcaa
     2821 ggccaacccg gatcactacg cgaagcggct gaccggccgg acgctgttca tgtacttcga
     2881 gaagccgtcg ctgcgcacgc gcgtgacctt cgaagccggg atgacgcagc tcggcggtca
     2941 tgcgatctac tacaccgccg ccgaaggcaa gatcggcgtg cgcgagagcg tcgaggacgt
     3001 ggcgcggaat ctcgaacggt gggtcgacgc tgcgatgtgt cgcactttca gtcacgaatt
     3061 gctgctcgag ctcgcccgct tcgccgagat ccccgtgatc aacggtctga ccgatcacct
     3121 ccacccgtgc caggcgctgg ccgactacat gacgctgcgg gagatcggcg gcgactcgct
     3181 ggcgggccgc cgcctggcct acgtcggcga cgggaacaac gtggcccact cgttgatgaa
     3241 cggcggtgca cgcctgggtg tgagcgtgac ggtgatcacg ccgcccggct acgagccgga
     3301 cgcgggggtc gtggagaccg cgcgcgcggc cgcgcagcag agcggtgcca cgatcgagat
     3361 cacccacgac atcgaggccg tgcgcggcgc gaacgcggtc tataccgacg tctgggcatc
     3421 gatgggccaa gaggacgagg cggcggagcg caagcgggta ttcgcgccgt accagatcca
     3481 ccgcggcgtg atggatctcg ccggtccgga gaccgccttc atgcattgcc tgccggctca
     3541 tcgcggcgag gaggtaacga gcgaagtggc cgacatgccg cgctcggtca tcttcgagca
     3601 ggcggagaac cgactccacg cccagaaggc ggtgctcctg gcgctgctcg gcggcgtcta
     3661 ggcgcggggg ccgcccttga tcgcggtgtg gaccgcgacg atgccgccgc tgagcgtcgt
     3721 ccatccgcag gcggcgaacc cggcgtcgcg gatccgtccg gcgagctcgg gcggatcggg
     3781 aaacgccgcg atcgtctgcg ccaggtaccg gtacggtccc gaggcgcccg cgataccgcc
     3841 gccgaggcgg ggcaggactc gcttcaggta gagccgatag accggtccga gccagggccc
     3901 cgccgggcgg ctgaactcga gcacgacgag ccggcctccg ggccggagca cgcgcccgat
     3961 ctcgcgcaac gcccgcaccc ggtcgtcgaa gttgcggacg ccgaaggcca ccgtgaccgc
     4021 gccgaatcgc tcgtcgggga agggcaggcg cagcccgtcg gcctccagcg tgacgcactt
     4081 ggacccggga cgccggcgcc ggaacttgac ctccgcccgg cgcagcattt cgtgggaaaa
     4141 gtcgcagcaa atcacccgct cgacttcgga ccggcgtgcg accgccaggc tcatgtcgcc
     4201 ggtcccgccg cacaggtcca acacctcccc ggcgggagcc cccgcgagct cggccgcggc
     4261 cctgcggcgc caggcatggt cgaggttggc cgacagcagg tggttcaaca ggtcgtaacg
     4321 acgggcgatg cggtcgaaca tcgaccgcac ttcggcgggg cctctcggct cgtccgggtt
     4381 cactcggtgg cactccctct tgcacgggcg acgagtggtc ataaatagca gccgccggcg
     4441 ccccgcccgc agaccggcca gggctcggct gccttgacgg cgcccctaag ccgatgatat
     4501 aagagcgctt tcgcttcagg gatatttgtg cgctcgggtc ggcccagggc gtatcatggt
     4561 gactcggcat gggaaccttc gtccccaagc ggggtgagat ccggcgcgac tggtggttgg
     4621 tcgacgccga cgggctgacg ctcgggcgct tggcgaccga ggtcgcgagc cgcttgcggg
     4681 gcaagcacaa gccgaccttc accccctacc tcgacaccgg cgatcacgtg atcgtggtca
     4741 acgccgagaa ggtcgtgctc accgggcgca agctggcgac caagacctac cgacgccaca
     4801 gcggttatcc cggcggcctg cacgaggcca cggcacgccg cctgctcgcc acgcacccgc
     4861 agcgggtgat cgagtccgcg gtgaagggca tgctgcccaa gggaccgctc ggccggcaga
     4921 tggcgcgcaa gctcaaggtc tacgccgggc cgtcgcaccc gcacgtttcg caacagccga
     4981 acaagatgga actggccgac gccgcgcgga accgttgagt gaccggcgcg gcgggacgag
     5041 gatgggcgtg agcgaaggaa cgctgcagta ctacgggacg ggtcgacgca agcgcgccgc
     5101 ggcgcgggtc tatctgcgac ccggatcggg caagttcgtg gtcaacgacc gcgccttcga
     5161 agactacttt cccagcgaga tgctgaagat ggtgatccgc cagccgctgg tcgtcaccga
     5221 gacgtcggac aagttcgacg tgctggttcg cgtggtcggc ggcggctgca cgggccaggc
     5281 gggcgcgctg cgtcacggca tctcacgcgc gctggtcaac tacaaccccg agctgcggcg
     5341 caagctgaag gaggcggggt tcctcaccag agatccgcgc aaggtcgagc ggaagaagta
     5401 cggtcaaccg ggagcgcgca agaggttcca gttctccaag cgttgacgtt cggagcgagc
     5461 ggacgtcgac gtccgtccga ggagggtttc ttggtaaccg tcacgatgaa ggaaatgctg
     5521 gaagccgggg ctcacttcgg ccaccagacg cggcgctgga atccgcggat gaagcgtttc
     5581 atcttcggcg cgcgcaacgg catttacatc atcgacctca agaagacctc ccgccatctg
     5641 cgcgaggcgc tggcgttcgt ctccgacctg tcggcgcgcg gcggcaccgt gctattcgtc
     5701 ggcaccaagc gtcaggccca agaagtcgtc gccgaagagg cgatccgcag cggcatgtac
     5761 ttcatcgacc agcgctggct cggcggcacg ctgaccaatt tcaccacgat tcgcaagagc
     5821 atcgcccgct tggccgagct cgaggagctg acctcgaacg acaaggccga ggcgttgacg
     5881 aagaaagaac gagccaagct cgacaaggaa cgcggcaagc tccagaagac gctgtccggc
     5941 atcaagacga tgtcgaagct gccgcaggcc gtcttcgtca tcgacccgca gaaggaacgg
     6001 atcgcggtgg ccgaggccaa caagctgggc attccggtgg tggcggtcgt cgacaccaac
     6061 tgcgacccgc aaccgatcga ctacccgatc ccgggcaatg acgacgcgat ccgtgcgatc
     6121 aagctgttcg cgggccgctt cgccgacgcg atcatcgagg gtcgccagat ttgggagtcg
     6181 gcacggcgcc agacggaaac cgagaccgag cagcaggtcg cggcccagcc gaagagcatc
     6241 gccgatcgcg tgcgggcccg cgaggcccgg cgcgagcgcg tgcgccagca agtccaggcc
     6301 aagcgtgtcc gccggtctcc gtcggacggc tcgggcgagg tggtcgccgc gggtgtcgac
     6361 aagaacgcca actccgactg acgctcggga atcgatcgtt cggccccgac gcggtgtcgc
     6421 tcgccgtgcc ggggcttttt gatgaagaac ggaccggggt cgagcgcccc gggcatcgat
     6481 ggagccgaca gcatgcaagt cacggcagcg atggtgaagg agctccggga gaagaccgga
     6541 gtcggcatga tgaagtgcaa ggccgcgctc agcgaggcga acggcgatct gtccgaggcc
     6601 gagaagatcc tgcgcaagca gggtgtcgcc gccgcggcga agaaggccga tcgcgcgacc
     6661 ggcgaggggc tggtcgaggc ctacatccac accggcggca agatcggcgt gatggtcgag
     6721 gtcaactgcg aaaccgactt cgccgcccgc agcgacgact tccgggaact ggtgcgcggc
     6781 atcgcgatgc acgtcgcggc ggccgccccg cgtttcgccc ggcgggagga cgtaccgcaa
     6841 gacgtgctcg agaccgagct cgaaatcgca cgcgagcagg ccctccgatc gggcaagccg
     6901 ccggcggtcg tcgacaagat cgtcgagggc aagatcgacc ggttctacgc cgagaactgc
     6961 ttgatcgagc agccctacgt ccgtgatccc gaccgcaccg tcggccaact gattcacgac
     7021 gcggtgagca agatcggcga aaacatccag ctgcggcgtt ttgtgcgcta cgtgctcggc
     7081 gaggcgggtt agcatcttca tcttcgcggg cgggccgtcg gcccgcgatc ccagggggcg
     7141 gaacgacacg tggaaccggc gtcagctcca aggtacagac gggtcctgct caagctctcg
     7201 ggcgaggcgc tgatcggcga ccagagttcg ggaatctccc ccgtgatcgt cgccgagctg
     7261 gccgaggaga tcgtccgtgt ccaccagctc ggtgtggagc tggcgctggt cgtcggcggc
     7321 ggcaatttca tccgcggcgc ccaggtgacc ggcatcgacc gcgtcacggc cgaccacatg
     7381 ggcatgctgg cgacgctgat caactcgctg gcgctgcagg acgcgatcga gcggcacggg
     7441 ctgatgacac ggctgctgtc ggcgctcgag atccggcagg tcgccgagcc gttcgtgctg
     7501 cgccgtgcgt tgcgccacct ggaaaagggc cgcgtcgtca ttttcgccgc gggaaccggc
     7561 aacccgtact tcaccaccga cagcgccgcc gcactgcgcg cgctcgaaat caaggccgag
     7621 gtcctgctca aggcgacgaa ggtcgacggt gtctacactg ccgacccggc gaccgacccc
     7681 ggcgcgaaga aactggacaa gatcgggtac ctggaaacgc tggagcgcgg cttgaaggtg
     7741 atggacacca ccgcgatctc gctgtgcatg gacaaccaac tgccgatcgt cgtgttcaac
     7801 gtgcgtgacc cgggcaacgt cacgcgcatc gtgcgcggcg aggcggtcgg ttcggtcgtg
     7861 tcggagtagt tgagatggac gaagcccggg tgctcgagac ggccggcgaa cggatggacg
     7921 cgagcgtcgc cgacgccaag cagaaactgg gaaccgtgcg aaccggtcgc gcgtcgctgg
     7981 cgatgctcga cgggatccgc gtcgactact acgggacgcc gaccccgctg aaccagatcg
     8041 ccaagctgtc ggtgcccgag ccgacgttga tcgtggccca gccgttcgat ccgtcgacct
     8101 tgacgctgat cgagaaggcg atcatggctt ccgacctggg cctcaacccg tccaacgacg
     8161 gcaaaatcgt ccgcatcccg atcccgccgc tgaccgagga gcggcgaaag cagctggtca
     8221 agcgtgtccg ggcgctgggc gaggaggcca agaccgcgat ccggcagatc cggcgcgacg
     8281 cgaacgagga gatcaagaag ctcgagaagg acagcgccat ctccgaggat gccgcccgac
     8341 gccacctcga cgagatccag aagaccaccg atcgacacac cgacaagatc gacgggctgt
     8401 gcaaggacaa ggaaaaggag ctgatggaga tcgcgtagtg cgcccggggg cccgcgccgc
     8461 gaccgagttc gccggtgtcg tcgtcgccgc cggactcgcg acccgcttcg gcggttcgac
     8521 gccgaagcag tttctcgagc tcggcgggcg cgcgctggtc gagcgggcgg tctcggcgat
     8581 cgcggcacac ccggcggtcg atcgcctggt gctggtgctg tccgccgatc ggctcgacga
     8641 cccgtacgtc tcgcggctcc gcgcgcgcca tcgcgggctg gcggtcgtcc gtgggggggc
     8701 gacgcgggcc gactcggtgg cggcggggct cgccgcggtc gaggcgcgct tcgtgctggt
     8761 ccacgatgcg gcacggccgc tggcatcgtt cgccgtggtc gatcgggtgg tcgaggcgac
     8821 gcgtcgattc ggtgcggcgg tacctgcgat cggcgtggcc gacacggtga aacgcgtcgc
     8881 tccgccgacg gaaccgggtg gggcgatggc cgtggtcgag acgatcgacc gctccccgct
     8941 ccgactggcc cagaccccgc agggcgcgag gctcgattgg ctgcgcgccg cgctcgagcg
     9001 cgcccgcgcc gaaggcgcgc cggttaccga cgagtcgtcg gcgctcgagc gggccggtca
     9061 ccgcgtggcg gtggtggagg gcgatgtgga gaatcgcaag atcaccgcag ccgcggatct
     9121 ggccgacgct cgccggcgac tgacgcccgc cgccgacggc ctgcgcgtcg gcagcgggtt
     9181 cgacatccac cgggtcggcg agggtcgacc gctggtgctg ggcggaatcg gcttcccggg
     9241 cgaggtcggt ctgaccggcc actcggacgc ggacgtcgtc ttgcacgccg tgatggacgc
     9301 gttgctcggc gccgccgggc tcggcgacat cggcttgctg tttccgccgg acgacccggc
     9361 ctgggccggt gcggacagcc gggatctggc atgcgtcgta gcacgccgga tcgacgaggc
     9421 cggcttcgcc gtcgtcaacc tcgacgtgac gttgctggcc gagcggccga agatcgcccc
     9481 gcggtcggtc gagatgcgcg acgcgatcgg ccgatcgctc ggtatccctt ccgatcgcgt
     9541 cggactcaag gcgacgacac tcgaacgtct gggtgcgctc gggcgcgggg agggcatcgc
     9601 ctgccacgcg gtcgcgttgc tcgtccgccg ggacgagcga tgagcggcga gcccgaggca
     9661 ccgcgcgtac gcttcgcacc gtctcccacc ggttcgctgc atctgggtaa cgcgcgcacc
     9721 gcgttgttca attggctcgt cgcgcggcag ggcggcggag tgttcgtact gcgcgtcgag
     9781 gataccgata tcgatcgcga gcgcgcgggc tcggaacggg cgattctcga ggacctgcgc
     9841 tggctcggcc tggactggga cgagggaccc gacgtcgacg gtccgtacgg gccctatcgt
     9901 cagtcggagc gcgcacaaag ttacgcggcc gccgcgcgcc tgctgctcga ccgcggtcgc
     9961 gcctaccgct gtttctgcga cgagcggcgg ctcgaggagg aacgcgagcg gcgggtcgcc
    10021 gagggccgtg ccccgcacta cgacggtcgc tgccggcgca tcgggccggc ggagtcgagc
    10081 gctcgttcgg agcgggaggc gttcgcggtc cgcttcctcg ccgccccggc ggacggtgcg
    10141 gacgcgagcg aacgggtgac cttcgtcgat cgtcttcgcg gcggggtcga gttcccgctg
    10201 gccgagatcg gcgatacggt gatcgttcgc cgggacggcc ggccgaccta caacttcgcc
    10261 gtcgtcgtcg acgacatcca gatgaagatc gacttggtcc tgcgcggcga cgaccatctg
    10321 tcgaacacgc cgcgccaggt gttgatcttc gaggcgctcg ggcatccgcc cccggagttc
    10381 gtccatctgc caatggtccg cggcccggac gggactcgat tgagcaagcg tcacggcgcg
    10441 gtgtcggtcg gcgagtaccg ccgggccggc taccccgtcg acgggatgat caacgggctc
    10501 gcgctgctcg gctggtcgcc gggcgacgac cgtgtcgtca tcgaccgttc cgagctggtc
    10561 gagcagttcc ggctcgatcg catcgtccgc tcgcccgcca cgttcgaccc gcagaagctc
    10621 gagtggatct gcgcgcagca catccagcgg atggacgagt ccgcactgct cgagccgttg
    10681 cgcgaacggc tcgccgaggc cgggcggctt ccagccgatt ggagccccgc cctgcgcgcc
    10741 tgggcccccg cgctcgtgcc gatcgtccgc accttcatca gtcgcttcga ccagatcccc
    10801 gagcgttgcg ctccggtgtt ccaccccggc gggcgtccac cggcggcgga cgccgagacg
    10861 gcgctggtgc aacccgtggc ggtcgaggtc gtccgtgcgc tgtgcgagct ggcctcggtc
    10921 cgtccgccga tcgacctcga agcgtggcac gcgctgcgcg acggcgtgcg cgagcgcgcc
    10981 ggcgttcgag gcaaggcgct gttccgaccg ctgcgcgtcg cgctcagcgg ctcggagtcg
    11041 ggaccggagc tcgatctgct cgtgccgttg atcgaaacgg gtcacgcggc ggctcccgat
    11101 cggatcgatg cggtcgcgac ccgcgcccgg cggacgttgg aatggctcgg cgccgcctga
    11161 acgggggcga ggacgacgcc gcggcgttcg tctacggcgt gcggccggtc ggcgagctgc
    11221 tcgcgtcgcg accgcaccag atcgagcgga tcttcgtcgc gcgcgacgcg cgcggaccgg
    11281 tgggacggat tctgcgcgaa gcgcgccacg cgggaatccc cgtctcgcac gtgacccgcg
    11341 agcgactggc gctgcgcctc ggccggcgga cggagagcca ggggatcgcg gccgtcgtgg
    11401 ccccacgcgc ctacgcggac agcgaggaga tctgccggcg ggctgcggcg gctgcggcgg
    11461 ggctgttggt gctcgtcgac cgcatgaccg accccgggaa cctcggctcg ctgctccgga
    11521 cgtgcgccgc ggccggcgtc gacggcgtgc tgctcagccg cgaaggcacc gtcggcctga
    11581 ccgcccacgt gctgaaggcc tcggccggcg ccgccgaacg tgtgccggtc gggcgggaga
    11641 cgaggccgcc gaaccgcgtt gccgagctca gaaagcgggg attcgaggtg ctcgcgctcg
    11701 atccgcgggg cgaggtcgcc tgggacgagc tcgacctacg gggcccgacg atgatcgtgg
    11761 ccggggggga ggctcggggc ccccggcccg ccgtggcgga cgcatgcagc agaaaagttg
    11821 caattcctct cgcggcgggc gtcgagtcgc tcaatgtcgc ggtcgccgtc gctgtgctac
    11881 ttttcgaggc gctcaggcaa cgccgggtcg cgtgcgggga ctcttgaaac cgttccgatc
    11941 gactgttaac atttgccgtt tgtgcgggct ggcgtagctc agttggtaga gcagcggttt
    12001 tgtaaaccgc cggtcggggg ttcaagtccc ttcgccagct ccaggcgggg cgatggcggc
    12061 ggcgccgggt gaacccgacc gacggagctg ggccgggcac gaacgacggg tggcgaccgg
    12121 ggcgaagccg aagcgggtgg cgaacccccg acatgacaag aaccacgacg cggaggggtg
    12181 cccgagtggt taaagggagc agactgtaaa tctgccggcc ttaggcctac gaaggttcga
    12241 atccttctcc ctccaccatc gtcgggcgcc gagttggcca gtggagccga gtgactgaaa
    12301 gcgagtgggc gggaatagct cagttggtag agcatcagcc ttccaagctg agggtcgcga
    12361 gttcgaatct cgtttcccgc tccaaggttg ttggcgaatc ggtgtccgag gacgttgcga
    12421 ggcccatgta gctcaggcgg tagagcactt ccttggtaag gaagaggtca ccggttcaag
    12481 tccggtcatg ggctccagcc ggacgtcaac ggtgtcgacg aaacgtggtt ggtgggaacc
    12541 gagggaccgc ggctaggtgc ccttgaagag gagaaacgat ggccaaggag aagttcgatc
    12601 gatcgaagcc gcacgtgaac gtggggacga ttggtcacgt ggatcacggg aagacgacgt
    12661 tga
//
LOCUS       DASPGN010000484         6726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1494531, whole genome shotgun sequence.
ACCESSION   DASPGN010000484 DASPGN010000000
VERSION     DASPGN010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494531"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VD788_14140"
     CDS             complement(<1..881)
                     /locus_tag="VD788_14140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HXV77450.1"
                     /translation="MTKQIDTIGPEQLIDRSRAFARPFRVADGDGFRLTDVDPAETMG
                     LDAEDKPRAKEALAAGVEALSGLQEMLYAQDRWAVLLIFQAMDAAGKDGAIKHVMSGI
                     NPQGCQVFSFKAPTSEELDHDFLWRCMRALPERGRIGIFNRSYYEETLVVRVHPELLA
                     AQKVPPKLVTDKIWKERFRDIRAFERYLARNGVLVRKFFLHVSKKEQKRRFLARLDDP
                     AKNWKFSINDAKERDHWDDYMKAYEQMIRHTASKQAPWFVVPADIKWFTRLVVAAAVI
                     DALASLELAFPKISDRQR"
     gene            1221..2387
                     /locus_tag="VD788_14145"
     CDS             1221..2387
                     /locus_tag="VD788_14145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV77451.1"
                     /translation="MCKPIGSTAETVALLSLLALVACTACREDGPPSAPAPLTVKAAA
                     VLTRDVPVYVEAIGETRGNTEIEIRARVEGFLESVDFEEGKFVDKGQLLYTIDPKPFE
                     AALAEAQARLAQAEADLARAHQDVVRYEPLVAKNAISRQEYETAVAIERAQRSAVEAA
                     RATATSAQLDLGYTRVVAPDAGLAGKTEVYPGTLVGRGQSTLLTRISKIDPIHTRFTI
                     AERDYLHYARRSEERRAEGDDLPLEMVLADGSLHPHPGRFVFIDRNVDPQTGTILLEA
                     AFPNPGAIVRPGQFARVRAAVDLKRDAVLVPQRAVQELQGIYNVAVIGDDGKVEMRMV
                     EPGERLDSMWVIDSGLEAGERIVVEGLQKVRPGVVVQAEMVSLDAPEPNPATGS"
     gene            2390..5551
                     /locus_tag="VD788_14150"
     CDS             2390..5551
                     /locus_tag="VD788_14150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HXV77452.1"
                     /translation="MAEFFIRRPIVAIVIAIIIVLLGLNTLRGLSFEQYPFLAPPTIR
                     ISATYQGAAAVAVEQSVATPVEQEVNGVEGMIYMKSSNTSDGRMQLDVNFEVGTDQDM
                     ANVLTQNRVSAAQARLPQEVIQQGVTVKKQSPSILMLISLYSPDGSYDANFLINYCGI
                     NLRDRLLRIPGIAQVDLFGGADYGMRIWLRPDKLAKLGLTPADVVSAIREQNLQAPAG
                     RVGAAPSPKDQEFTYTVSAPGRLVTAEEFADIIIRETDTGGTIRISDVGRVALGAQNY
                     DSFGRLDGQPAGAMALYLLPGANQLRAAQTIYQTMEEAKGFFPADMDYRIVYDTTPAV
                     EASIESIFHTFIEALVLVTLVVFVFLQNLRATIIPLLTVPVSLIGTFIFFPLLGFSVN
                     TLSMFGLVLAIGIVVDDAIVVVEAVMHHIEHGMSPRDATVQAMKEVSAPVIGIALILS
                     AVFVPVAFIPGLTGRMYQQFALTIAISVLLSAFSALSLSPALAAMLLRPAKPARGPLG
                     WFFAKFNLAFERVTRGYLGGARLLVRRSALTIVIVAVVAVGAGLFGGALPAGFIPEED
                     QGILGVNVTLPAGASLERTSAVLTQVEEILGRTDGIESYQTIGGYGVVTSTYQPNFGT
                     VFLRLDPWEERHAAELHARSFLRRLSAEFARIPDAIIFPFNIPTISGFGASAGFNFLI
                     QDRSGSLTVEQLGELSRRFIDAARERPEIGNVFTSFDPRYPQVKVDLDREKARKLGVP
                     IDEAFQALAASLGGTYVNDFNRFGRLFRVYVQAEADYRRRPEDIGQIYVRSATTGDMV
                     PLSTLVTITSQGGTELTNRFNLQRSVEFNGVPGRGYASGQALAALEQVFAETMPPELG
                     YGYSQLSYQEKTAAPALPTLVAAIVVVFLLLAALYESWRLPWAVLLGSPLVALGAFFG
                     VWLLGYDNNVYVQIGNIMLIGLAAKNAILIVEFAKAKHEEGLDLAQAALESARLRFRP
                     ILMTAFAFILGVVPLMKASGAGAGAQNVMGTAVFFGMLVATALGVFLIPGNFTFIEGL
                     GAARRERRKAVAAAPPAPAEGAH"
     gene            5553..>6726
                     /locus_tag="VD788_14155"
     CDS             5553..>6726
                     /locus_tag="VD788_14155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXV77453.1"
                     /translation="MARRLPWIALLVALSGCKVGPDYARPPVVTPEQWRATAADDASI
                     ANVPWWELFDDEELQRLIRTALAENRELKIAVERIEEARARYGFSRADLYPRLDVNAV
                     GGGLRVSEGSLTHTPEGLSDDNTTELYSLNVGLSWELDFFGRIRRANEAEQANLFATE
                     EARRAVTIALVADVARAYVDLRDLDLRLEIARRTLESRREYVVLARDRFEGGITPELD
                     WRQAEAELHRVLALVYELEKLVAQKENEISYLIGNNPGAVPRGRSVDRQPIPPQVPAG
                     LPAVLLEQRPDVREAEQRLISATALIGEAKAQLYPRISLTGAYGFASTDLGDLLDSSS
                     ESWNFLGGLVQPLFNAGKNKRRVEVRQSQQRQAVHAYERTLLQAFREVEDALIALRK"
BASE COUNT         1058 a         2279 c         2282 g         1107 t
ORIGIN      
        1 cgctggcggt cgctgatctt cgggaacgcc agctccagcg aggccagcgc gtcgatcacc
       61 gcggcggcga cgacgaggcg cgtgaaccac ttgatgtcgg ccggcacgac gaaccacggg
      121 gcctgcttcg acgcggtgtg ccggatcatc tgctcgtacg ctttcatata gtcgtcccag
      181 tggtcgcgtt ccttggcgtc gttgatcgag aacttccagt tcttggccgg gtcgtccagc
      241 cgcgcgagaa accggcgctt ctgctccttc ttcgacacgt gcaggaagaa cttgcgcacc
      301 agcacgccgt tgcgggccag gtagcgctcg aacgcgcgga tgtcgcgaaa ccgttccttc
      361 cagatcttgt cggtcacgag cttcggcggg accttctgcg ctgccagcaa ctcggggtgg
      421 acgcggacga ccagcgtttc ttcgtagtag ctccgattga agatgccgat gcgcccgcgc
      481 tcgggcaacg cgcgcatgca gcgccagagg aagtcgtgat cgagctcctc actggtcggc
      541 gccttgaacg agaagacctg acagccctgc gggttgatcc cgctcatcac gtgcttgatc
      601 gcgccgtcct tgcccgccgc atccatcgcc tggaagatga gcagcaccgc ccaccgatcc
      661 tgcgcgtaga gcatctcctg cagaccggac aacgcctcga cacccgccgc cagcgcctcc
      721 ttggcgcgcg gtttgtcctc cgcgtcgaga cccatcgtct ccgccggatc gacatcggtc
      781 agtcggaaac cgtcgccatc ggcgacgcgg aacggacggg cgaaggcccg ggacctgtcg
      841 atcagctgct ccggcccgat cgtgtcgatc tgcttggtca tcgcggacgc cccctgtggc
      901 gagtggcggg tgagccgact cggttccccc gccgtgcgga tggcaatggt gtagcatgcc
      961 acccgaccgg gcgcccgtcc gcccgtgccg acggaggtca agcaaccgat gagctctcga
     1021 ctctcgccga cgtggctcgc cggcagacga ttggcctcga cgggcagcgg cggaatgacc
     1081 gacctgaagg gcggcaccga gcgcgcggcg cgcgacgtcg cagcccacgt tggagccgct
     1141 gaacaatgtg ggcgtctttc catagtgagc ccggcgtagg cgcggtcccc cgggatctcg
     1201 ggtaacgagg tgccgtcgtc atgtgcaagc caatcggttc aacggctgag acggtcgcgt
     1261 tgctgtcgct gctcgcgctc gtcgcctgca cggcgtgtcg cgaggacggc ccaccgtccg
     1321 cccccgcacc gctcacggtc aaggcggcgg cggtgctgac gcgagacgta ccggtgtacg
     1381 tcgaggcgat cggcgagacg cgcggcaaca ccgagatcga gatccgcgcg cgtgtcgagg
     1441 gctttctcga aagcgtggac ttcgaggaag gcaagttcgt cgacaagggg cagttgctgt
     1501 acacgatcga ccccaagccg ttcgaggcgg ccctggccga agcccaggcg cggttggccc
     1561 aggccgaagc cgatctcgcg cgcgcccatc aggacgtggt gcgctacgaa ccgctcgtcg
     1621 ccaagaacgc gatctcgcgc caggagtacg agacggcggt cgcgatcgag cgcgcgcaac
     1681 gttcggcggt cgaagcggca cgggcgaccg ccacgagcgc ccagctcgac ctgggctaca
     1741 cccgggtcgt ggcgcccgac gcggggctcg cgggcaagac cgaggtctat ccggggacgc
     1801 tggtcggccg gggccagagc acgctgttga cgcgcatctc gaagatcgat ccgatccata
     1861 cccgcttcac catcgccgag cgggactacc tgcattacgc tcggcggagc gaggagcgcc
     1921 gagccgaagg cgacgatctg ccgctcgaga tggtgctggc cgacggatcc ttgcaccccc
     1981 atcccggccg cttcgtcttc atcgaccgca acgtcgatcc gcaaaccggc acgatcctgc
     2041 tcgaggccgc gttccccaac cccggcgcga tcgtccggcc gggacagttc gcgcgggtac
     2101 gggcggcggt cgatctgaag cgcgacgcgg tgctggtccc gcagcgcgcc gtgcaggagc
     2161 tgcaggggat ctacaacgtc gcggtgatcg gcgacgacgg caaggtcgag atgcggatgg
     2221 tcgagcccgg ggaacgcctc gactcgatgt gggtcatcga ttcgggcctc gaagccggtg
     2281 agcggatcgt cgtcgagggg ctgcagaaag tacggccggg cgtggtcgtc caggccgaaa
     2341 tggtcagcct cgacgcgccc gagccgaatc cggcgaccgg gagctagcga tggccgagtt
     2401 cttcatccgc cggccgatcg tcgcgatcgt catcgcgatc atcatcgtct tgctcggact
     2461 gaacacgctg cgcgggctgt cgttcgagca gtacccgttc ttggcgccgc cgacgatccg
     2521 catctcggcg acctaccagg gggcagcggc ggtggccgtc gaacagtcgg tcgcgacgcc
     2581 cgtcgagcag gaggtcaacg gcgtcgaggg catgatctac atgaagtcgt cgaacacgag
     2641 cgacggccgg atgcaactcg acgtcaactt cgaggtcggc accgatcagg acatggccaa
     2701 cgtgctgacg cagaaccggg tgtcggcggc ccaggcgcgg ctgccccagg aggtgatcca
     2761 gcagggcgtg acggtcaaga agcagagccc gagtatcttg atgctgatct cgctctactc
     2821 gcctgacggg agctacgacg ccaacttcct gatcaactac tgcggcatca acctgcgcga
     2881 ccggttgttg cgaattccgg gcatcgcgca ggtcgatctg ttcggcggcg ccgactacgg
     2941 catgcggatc tggctgcgac cggacaagct ggccaagctc ggcctgaccc ccgccgacgt
     3001 ggtgtcggcg atccgcgagc agaacctcca ggctccggcc gggcgcgtcg gggccgcgcc
     3061 gtcgccgaaa gatcaggagt tcacctacac ggtgagcgca ccgggacgcc tggtgaccgc
     3121 cgaggagttc gccgacatca tcatccgcga gaccgacacc ggtgggacga tccgcatctc
     3181 ggacgtcggt cgcgtcgcgc tcggcgcgca aaactacgat tcgttcgggc gactcgacgg
     3241 gcagccggcc ggcgcgatgg cgctctacct gttgcccggc gccaaccagc tccgtgccgc
     3301 gcagacgatc taccagacga tggaggaagc gaagggcttc tttccggccg acatggacta
     3361 ccgcatcgtc tacgacacga cgccggcggt cgaggcgtcg atcgagtcga tcttccacac
     3421 cttcatcgaa gcgctggtcc tcgtcacgct ggtggtcttc gtttttctgc agaacctgcg
     3481 cgcgacgatc atcccgctgc tgaccgtacc cgtttcgctg atcggaacgt tcatcttctt
     3541 cccgttgctc ggcttctccg tcaataccct gtcgatgttc gggctcgtgc tcgcgatcgg
     3601 catcgtcgtc gacgacgcga tcgtggtcgt cgaagcggtg atgcaccaca tcgagcacgg
     3661 aatgagccca cgggacgcga cggtccaggc gatgaaggaa gtgtcggcgc ccgtcatcgg
     3721 cattgcgctg atcctgtcgg cggttttcgt cccggtcgcg ttcatcccgg gcctgaccgg
     3781 acggatgtac cagcagttcg ccctgacgat cgcgatctcg gtgctgctgt cggcgttcag
     3841 cgcgctgtcg ctgtcccccg ccctggccgc gatgctgctg cgaccggcca agcccgctcg
     3901 cggcccgctc ggctggttct tcgcgaagtt caacctggcg ttcgaacgcg tgacgcgtgg
     3961 ctacctcggc ggggcacgtc tgctcgtgcg ccgctcggcg ttgacgatcg tgatcgtcgc
     4021 ggtggtggcg gtgggcgcgg gtctgttcgg cggcgcgttg ccggcgggct tcatccccga
     4081 ggaggaccag ggcatcctgg gcgtcaacgt cacgttgccc gccggcgcct cgctcgagcg
     4141 gaccagcgcc gtgctgaccc aggtggagga gatcctcggg cgcaccgacg gcatcgagtc
     4201 ctaccagacg atcggtggct acggcgtggt caccagcacc taccagccga atttcggcac
     4261 ggtgttcctg cgcctcgacc cgtgggagga gcggcacgcg gccgagctgc acgcacgcag
     4321 cttcctccgc cgactgtcgg ccgagttcgc ccgcatcccc gacgccatca tctttccctt
     4381 caacatcccg acgatctccg gcttcggcgc ctcggccggt ttcaacttcc tgatccagga
     4441 ccgcagcggt agcctgacgg tcgagcagct cggcgagctg agccggcgct tcatcgacgc
     4501 ggcgcgagag cgtcccgaga tcggcaacgt ctttacctcg ttcgacccgc gctacccgca
     4561 ggtcaaggtc gacctcgacc gggagaaggc gcgcaagctc ggcgtcccga tcgacgaggc
     4621 gttccaggcg ctggcggcca gcctcggcgg cacctacgtc aacgacttca atcgcttcgg
     4681 ccggctgttt cgcgtctacg tgcaggccga ggccgactac cgccggaggc ccgaggacat
     4741 cgggcagatc tacgtgcgca gtgcgacgac cggtgacatg gtccccctct cgacgctggt
     4801 cacgatcacc tcgcagggcg ggaccgagct gaccaatcgc ttcaatctgc agcgctcggt
     4861 cgagttcaac ggcgtgccgg gccgaggata cgcctcgggt caggccctgg ctgcgctcga
     4921 gcaggtgttc gccgaaacga tgccgcccga gctgggctac ggctactcgc agctctcgta
     4981 tcaggagaag acggcggcgc cggcgctccc gacgctggtc gccgcgatcg tcgtggtctt
     5041 cctgttgctg gcggcgctct acgagagctg gcggctgccg tgggccgtgc tgctcggctc
     5101 gccgctggtc gcgctcgggg cgttcttcgg cgtctggttg ctcggttacg acaacaacgt
     5161 ctacgtccag atcggcaaca tcatgctgat cggccttgcc gccaagaacg ccatcctgat
     5221 cgtcgagttc gccaaggcca agcatgagga gggactcgac ctggcacagg ccgcgctcga
     5281 atcggcgcgg ctgcgtttcc gaccgatcct gatgacggcg ttcgccttca tcttgggggt
     5341 ggtgccgctg atgaaggcca gcggcgccgg cgccggcgca cagaacgtca tggggaccgc
     5401 ggtgttcttc ggcatgctgg tcgccaccgc gctcggcgtc ttcctgatcc cgggcaactt
     5461 caccttcatc gaaggcctcg gtgcggcgcg ccgcgagcga cgaaaagcgg tcgccgccgc
     5521 gccacccgcg ccggccgagg gagcgcactg acgtggcgcg ccggctcccg tggatcgccc
     5581 tgctcgtcgc gctctccggc tgcaaggtcg ggccggacta cgcccgtcct ccggtcgtca
     5641 cgcccgaaca gtggcgggcg acggcggccg acgatgcctc gatcgccaac gtcccgtggt
     5701 gggagctgtt cgacgacgag gagctgcaac gactgatccg caccgcgttg gcggagaatc
     5761 gcgagctgaa gatcgcggtc gagcggatcg aggaggcccg ggcacgctac ggcttcagcc
     5821 gtgccgatct ctatcctcga ctggacgtca acgccgtcgg cggcggcctg cgcgtcagcg
     5881 aaggcagcct gacccacacg ccggaggggc tgtccgacga caacacgacg gagctgtatt
     5941 cgctgaacgt cgggctgtcg tgggagctcg acttcttcgg acggatccgg cgcgccaacg
     6001 aggccgagca ggccaacctg ttcgcgaccg aggaggcgcg gcgcgccgtc acgatcgcgc
     6061 tggtggccga cgtggcccga gcctacgtcg acctgcggga cctcgacctg cgtctcgaga
     6121 tcgcgcggcg cacgctcgag tcgcggcgcg agtacgtcgt gctggcgcgc gaccggttcg
     6181 agggcggcat cacgcccgag ctcgactggc gtcaggccga ggccgagctg caccgcgtcc
     6241 ttgcgttggt ctacgaactg gagaagctgg tcgcgcagaa ggagaacgag atctcgtacc
     6301 tgatcggcaa caaccccggg gcggttccgc gggggcggtc ggtcgatcgc caaccgattc
     6361 cgccgcaggt cccggccggt ctgccggccg tcctgctcga gcagcggccc gacgtgcgcg
     6421 aggccgagca gcgcctgatc tcggccacgg cgctgatcgg ggaggccaag gcgcaactct
     6481 acccgcggat cagcctgacg ggtgcgtacg gtttcgccag caccgacttg ggcgatctgc
     6541 tcgacagctc gagcgagagc tggaacttcc tcgggggcct cgttcagccg ttgttcaacg
     6601 cgggcaagaa caagcggcgc gtcgaggtcc gccagtcgca gcaacgccag gcggtccatg
     6661 cttacgagcg gacgctgttg caggcgttcc gcgaggtcga ggacgcgctg atcgccctac
     6721 gcaagg
//
LOCUS       DASPGN010000485         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_910070, whole genome shotgun sequence.
ACCESSION   DASPGN010000485 DASPGN010000000
VERSION     DASPGN010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910070"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VD788_14160"
     CDS             <1..474
                     /locus_tag="VD788_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HXV77454.1"
                     /translation="RARVRLARLDRWPLYVQGEGSKAALVERFRATPRAVLLGTASFW
                     HGVDVPGRALSTVVVDKLPFDVPSDPLVAARVERIREQGGDPFGSYQLPMAVLDLKQG
                     LGRLLRSREDRGLLCVLDPRIRTRRYGKVFLRSLPPYRVVHDLDECAAFLAAGTR"
     gene            530..1066
                     /locus_tag="VD788_14165"
     CDS             530..1066
                     /locus_tag="VD788_14165"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HXV77455.1"
                     /translation="MRPDAQIEIVQDDITRLAVDAIVNAANRTLLGGGGVDGAIHRAA
                     GPGLLAECRRIGGCPTGEARITGGHTLPARWVIHTVGPVWRGGQAGEPMLLAACYHNS
                     LTLALEHELQSIAFPAISCGAYGYPAELAARVAVEQIDRFLAVHRAPRRVLLVAFDAE
                     MARHYAAARRLGRRRQES"
     gene            1113..2333
                     /locus_tag="VD788_14170"
     CDS             1113..2333
                     /locus_tag="VD788_14170"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HXV77456.1"
                     /translation="MDLDSMARLALPLAMLVVLTASCRDSSPTPTPEEAAIELAQRVL
                     ILDTHIDLPFRLQEQWEDVGQRTATGDFDYPRARSGGLDVAFMSIYVPASYQESGGAR
                     LFADELIDSVERLVREHPDKFELVRGTDDIRGRAGDGKVLLAMGMENGAPIEHDLANV
                     RHFHDRGVRYVTLAHSKANAISDSSYDEDRPWGGLSPFGREVVVEMNRVGMMIDVSHL
                     SDDATHQAIAASRTPVIASHSSCRHFTPGWERNLSDELIREIAASGGVIQINFGSGFV
                     DDDYRRAWLARHEQVESYFEEHQIDPESDEGKAFSKRYVRENPIEPVRAERVADHIDH
                     VVGLVGIDHVGLGSDFDGLGDSLPHGLRDVSHYPNLLRILSERGYSPAEIEKICGANL
                     LRVWGEVEAAAAPS"
     gene            2330..3091
                     /locus_tag="VD788_14175"
     CDS             2330..3091
                     /locus_tag="VD788_14175"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV77457.1"
                     /translation="MTRPTERFSDRVTDYVRARPGYPASIPALLAERCGLTPDWQVTD
                     VGSGPGNLARLFLDNGNQVTGVEPNREMRLAGERMLAGYPRFTSVAGRAEATGLPSRS
                     CDLVSAGQAFHWFDPGPARAEFARISRRPGWVVLVWNDRAERASATMCGYEELLRRYG
                     TDYRQAHHRRIGATEIAAFYAPRTFERIELNHSQTLDRDGLRARMLSSSYTPSEGTPQ
                     RAELVAAVDRLFDHHERQGRVDLIYRTRVYLGRSD"
BASE COUNT          498 a         1061 c         1143 g          522 t
ORIGIN      
        1 cgggcgcgcg tgcggctcgc gcggctggac cgctggccgc tgtacgtgca gggagagggc
       61 agcaaggcgg cgctggtcga gcgtttccgc gccaccccgc gcgcggtgct gctcggcacc
      121 gcgtcgttct ggcacggagt cgacgtaccg gggcgcgcgc tgtcgaccgt cgtggtcgac
      181 aagctaccgt tcgacgtgcc gagcgatccg ctcgtcgcgg cgcgcgtcga acggatccgg
      241 gagcagggag gcgacccgtt cgggagctac cagctgccga tggcggtcct cgacctgaag
      301 caagggctcg gccggctgct gcgcagcagg gaggaccgcg gcttgctgtg cgtgctcgac
      361 ccgcgaatcc ggactcggcg ctacgggaag gtgttccttc gatcgctccc gccgtaccgg
      421 gtcgttcacg acctggacga gtgtgcggcg ttcctcgcag ccggaacgcg ttgagcgcgg
      481 tgcgtacggt cgaccgccga agtcgagcgc gtaccgtggt gctcgagccg tgagacccga
      541 cgcccagatc gagatcgtac aggacgacat cacccgactc gcggtggacg cgatcgtcaa
      601 cgccgccaat cggacgctgc tcggcggcgg gggcgtggac ggagccatcc accgcgccgc
      661 ggggccgggg ctgctcgccg agtgccgccg gatcggcggc tgcccgacgg gagaagcgcg
      721 catcaccggc ggtcacacac tgcccgcccg ctgggtgatc cacacggtcg gtccggtttg
      781 gcgcggcggt caggcgggag aaccgatgtt gctcgcggct tgctaccaca acagcctgac
      841 gctggccctc gagcacgagc tgcagtcgat cgccttcccg gcgatcagtt gcggcgcata
      901 cggctacccg gcggagctcg cggcgagagt cgcggtcgag cagatcgacc gttttctcgc
      961 ggtgcaccgc gcgccccgcc gggtcttgct cgtcgccttc gacgcggaga tggctcgtca
     1021 ttacgccgcg gcgcgcagac ttggccgacg gcggcaggag tcgtaagatc ggcgaatcgc
     1081 agtcgtgggc agccgcccgg aaggagcgtg tgatggatct cgactcgatg gcccgcctcg
     1141 cgttgccgct cgcgatgctg gtcgtgctga ccgcctcctg ccgcgactcc tcgccgacgc
     1201 cgactcccga ggaggcggcg atcgagctcg cgcagcgggt gctgatcctc gacacccaca
     1261 tcgacctgcc gttccgtctg caagagcagt gggaagacgt cggccaacgc accgcaaccg
     1321 gtgatttcga ctatccgcgc gcacggagcg gcggtctcga cgtcgcattc atgtcgatct
     1381 acgtccccgc ctcctaccag gaatcgggcg gcgcacgatt gttcgccgac gagttgatcg
     1441 actcggttga gcggctcgta cgggagcatc cggacaagtt cgagctggtc cgggggacgg
     1501 acgacatccg agggcgcgcg ggcgacggca aggtcctgct ggcgatgggg atggaaaacg
     1561 gcgcgccgat cgagcacgac ctggcgaacg tgcgccactt tcacgatcgc ggcgtgcgat
     1621 acgtcacctt ggcccattcg aaggcgaacg cgatcagcga ctcgtcctac gacgaggacc
     1681 gcccgtgggg tggactcagt ccgttcggtc gcgaggtggt ggtcgagatg aaccgggtcg
     1741 gcatgatgat cgacgtctcc cacctgagcg acgacgccac gcaccaggcg atcgcggcgt
     1801 cacggactcc ggtgatcgcg tcccattcct cgtgccgtca cttcacgccg ggctgggagc
     1861 gcaacctgag cgacgagttg atccgcgaga tcgccgcgag cggcggcgtg atccagatca
     1921 acttcggatc gggattcgtc gacgacgatt accggcgggc ttggctcgca cgccacgagc
     1981 aggtcgagag ctacttcgaa gagcatcaga tcgacccgga atccgacgag ggcaaggcgt
     2041 tttccaagcg gtacgtgcgc gagaacccga tcgagccggt gcgcgcggag cgtgtcgcgg
     2101 accacatcga ccacgtcgtc gggctggtcg gcatcgacca cgtcgggctg ggctccgatt
     2161 tcgacgggtt gggcgattcg ttaccgcacg ggttgcgcga tgtatcgcat taccccaacc
     2221 tgctgcgcat cctgtcggag cgtgggtatt cgccagcgga gatcgaaaag atctgtggtg
     2281 ccaacctgct gcgcgtctgg ggcgaggtcg aggcggcggc ggcgccgtcg tgacccgacc
     2341 gaccgagcgg ttttccgatc gggtcacgga ctacgtgcgg gcgcgtccgg gctatcccgc
     2401 gtcgataccg gcgttgctcg ccgagcgttg cgggttgaca cccgactggc aggtgaccga
     2461 cgtcggttcg ggacccggca acctggcgcg cctgtttctc gacaacggca accaggtaac
     2521 cggggtcgag cccaaccggg agatgcgcct cgccggcgag cggatgctcg ccggctatcc
     2581 acgcttcacc agcgtggccg gacgtgccga ggcgaccggc ctgccgtccc gcagctgcga
     2641 tctggtcagc gccggtcagg ctttccactg gttcgacccc ggccccgccc gtgcggaatt
     2701 cgctcgcatc tcgcgccgac ccggctgggt cgttttggta tggaacgacc gggcggagcg
     2761 ggcgagcgcg acgatgtgcg gctacgagga actgctgcgc aggtacggga cggactaccg
     2821 gcaggcccac caccggcgca tcggggcgac ggagatcgcg gcgttctacg ccccgcgcac
     2881 gttcgagcgg atcgagctga accacagcca gacactcgat cgggacgggc tgcgtgcgcg
     2941 catgctgtcg tcgtcctaca ctccgagcga gggtacgccg cagcgcgccg agctcgtcgc
     3001 ggcggtcgac cggctgttcg accaccacga gcggcagggc agggtcgacc tgatttaccg
     3061 gacgcgggtc tacctcggac gctcggacta ggagcctgtc cgagtaatcg gattcgattg
     3121 tagtttccag ttcctgcgga ttggcgggtc tggaataatc ggggcatgga caagaccttc
     3181 cgcccgtacg atccggatca gctgttgttg ttgcctcccg cgct
//
LOCUS       DASPGN010000486         2312 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_675488, whole genome shotgun sequence.
ACCESSION   DASPGN010000486 DASPGN010000000
VERSION     DASPGN010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675488"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VD788_14180"
     CDS             <1..376
                     /locus_tag="VD788_14180"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HXV77458.1"
                     /translation="PRDAGLELVERSFAEGRKLYGWEVDDRVRAVVTRGGYGEAFLHR
                     TGHSIGIDDVHGNGANIDNLETKDERMIVPGTCFSIEPGIYLDGRMAVRSEIDVYVRD
                     DGSVEVCGPVQRELIRIGGGER"
     gene            complement(373..1023)
                     /locus_tag="VD788_14185"
     CDS             complement(373..1023)
                     /locus_tag="VD788_14185"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV77459.1"
                     /translation="MSAAEVRRLWIPGPAGRLEAALRVAACPRATAVVAHPHPLHGGT
                     LHNPVVFHAERRLNRAGLTTLRFNFRGVGESQGLHDAGRGEVDDLSAAASWVRGLADE
                     VPLVVVGYSFGAACALRLARVDRSVRAVVCIGLPLGGGVTNEVDVLTRPVAVVQAERD
                     EFGEPDAVRRWLARADSAGRLYVVERTTHLFPGRAREAADRVGLAVEHLLRRIDAG"
     gene            complement(1020..1970)
                     /locus_tag="VD788_14190"
     CDS             complement(1020..1970)
                     /locus_tag="VD788_14190"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXV77460.1"
                     /translation="MARLKRSRLLDLAGLAMAGAGAVGLVVRLTVRDGWPLAANLFYV
                     LPTLMIAVLFGLASVVWWVNRRRVTALVCAGLTLLMSGVWGRDAAYFGRGCEPSPASF
                     RVLLWNTARGVAGWPRVAEQMARADPDLIGLVEAGGSGEDAKAFWLARFPQHEAYMAG
                     AGMVILVRGRIVEHRTLPVAGTSRCAVVEAEVRGSRLRVVVLDAIVRPFSDRREVVRQ
                     VFELARSASAVPTIVMGDFNTPVDSVWFEDPRGEYVHAFEFAGRGMLGTWPVPLPVLA
                     IDHIWVSRGLDVRCARIGWSWFSDHRPMMADLSFTGTNDR"
     gene            complement(1990..>2312)
                     /locus_tag="VD788_14195"
     CDS             complement(1990..>2312)
                     /locus_tag="VD788_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXV77461.1"
                     /translation="SAGYRLAYCEDGDGTVVAVAGFRVSENLAWGKFLYVDDLVTDAT
                     RRSGGHGAGLLAWLRRTAVDAGCAELHLDSGVQRFAAHRFYTRHRMEITSHHFRLELT
                     EVVS"
BASE COUNT          395 a          847 c          793 g          277 t
ORIGIN      
        1 cccccgcgac gcgggcctcg agctggtcga acgctcgttc gccgagggac ggaagctcta
       61 cggctgggag gtcgacgacc gcgtccgcgc cgtcgtgacc cgcggcgggt acggcgaggc
      121 gttcctgcac cgcaccggcc acagcatcgg cattgatgac gtccacggca acggcgccaa
      181 catcgacaac ctggagacca aggacgagcg gatgatcgtg ccgggtacct gcttttcgat
      241 cgagccgggg atctacctcg acgggcggat ggcggtgcgc agcgagatcg acgtctacgt
      301 gcgggacgac ggctcggtcg aagtgtgcgg cccggttcag cgcgagctga tccgcatcgg
      361 cggcggcgag cgctagccgg cgtcgatccg ccggagcagg tgctcgacgg cgagcccgac
      421 ccggtcggcc gcctcgcgcg cgcgtccggg gaacaggtgc gtcgtccgct cgacgacgta
      481 cagccgtccc gcggaatccg cgcgtgcgag ccagcgccgc accgcgtccg gctcgccgaa
      541 ttcgtcccgt tccgcctgga ccaccgccac cggccgggtg agcacgtcga cctcgttcgt
      601 gacaccgccg ccgagcggga gaccgatgca gacgacggcg cggaccgagc ggtcgacacg
      661 tgccaatcgc agcgcgcacg ccgctccgaa cgagtagccg acgaccacca gcggcacctc
      721 gtcggccaga ccgcgcaccc aggacgccgc ggcggacaga tcgtccacct cgccgcggcc
      781 ggcgtcgtgc agcccctgac tctcaccgac gccacggaag ttgaatcgaa gcgtggtcag
      841 cccggcccgg ttcagccgac gttcggcatg aaacacgacc gggttgtgca gcgtgccgcc
      901 gtgtaacgga tggggatgcg cgaccacggc cgtcgcccgc ggacacgccg cgactcgcag
      961 cgcggcctcg agcctgcccg cgggaccggg gatccacagc cgccgcacct cggcggcgct
     1021 caccggtcgt tcgtcccggt gaagctcagg tcggccatca tcggccgatg gtcggaaaac
     1081 cagctccaac cgatacgcgc gcagcggacg tcgagcccgc gcgagaccca gatgtgatcg
     1141 atcgcgagga ccggcagcgg gacgggccag gtgccgagca tcccgcggcc ggcgaactcg
     1201 aatgcgtgga cgtactcgcc acgcggatcc tcgaaccaga cggagtcgac cggagtgttg
     1261 aagtctccca tcacgatcgt cgggaccgcg ctcgccgaac gcgccagctc gaagacctgg
     1321 cggacgactt cccgccggtc ggagaacggg cgcacgatcg cgtcgagcac gaccacgcgg
     1381 agacgcgatc cacgcacttc ggcctcgacc accgcgcagc ggctggtccc ggcgaccggt
     1441 agcgtgcggt gctcgacgat ccgcccgcgg accaggatca ccatgccggc gccggccatg
     1501 taggcctcgt gctgcgggaa ccgggccaac cagaaggcct tggcgtcttc acccgatccg
     1561 ccggcctcga ccagaccgat caggtcgggg tcggcacgcg ccatctgttc ggcgacgcgc
     1621 ggccagccgg cgacgccgcg cgccgtgttc cacagcagca cgcggaacga cgcgggcgac
     1681 ggttcgcagc cgcggccgaa gtaggcggcg tcgcgacccc agacacccga catcaacagc
     1741 gtcaggccgg cgcatacgag cgccgtgacg cggcgacgat tgacccacca gaccacgctg
     1801 gcgagaccga acagcacggc gatcatcaac gtcggaagga cgtagaacag gttcgccgcc
     1861 agtggccaac cgtcccgcac ggtcaaccga acgaccagcc cgacggcgcc ggcgccggcc
     1921 atcgccagcc cggccagatc gagcagtcgg gatcgcttca gccttgccat ctcggtccgg
     1981 gggcgccgat cacgatacca cctcggtgag ctcgagccga aaatggtggc tcgtgatctc
     2041 catccggtgc ctcgtgtaga accggtgcgc ggcgaagcgc tggacgccgg agtcgagatg
     2101 cagctccgcg cagccggcgt cgaccgccgt tcggcgcaac caggcgagca gccccgcgcc
     2161 gtggccgccg gagcgccgcg tggcgtcggt gaccaggtcg tcgacgtaga ggaacttgcc
     2221 ccaggccagg ttctccgaca cccgaaaacc ggcgactgcg acgaccgtcc cgtcaccgtc
     2281 ctcacaatag gccagccggt agccggcgga ct
//
LOCUS       DASPGN010000487         5017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1260711, whole genome shotgun sequence.
ACCESSION   DASPGN010000487 DASPGN010000000
VERSION     DASPGN010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260711"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1537)
                     /locus_tag="VD788_14200"
     CDS             complement(71..1537)
                     /locus_tag="VD788_14200"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HXV77462.1"
                     /translation="MPGLPFASTVPSGTATAPPPSIPLSKPAAVSPFGLSADGVHRLA
                     LEAFRIGNRGRLSLCEALRVLGETRLHLELGFPSVAAYADRFFQLRRAETFECVRVAR
                     ALLELTELREAFGQGRIGWSVLKAVTRVASPASQSAWIDFAREHDVARTLAEARDALR
                     RGRDKPRDSSFGLPNLDQKLVLRFSRSDLEKVRTWIEGACATVAARTGVEEVTLEQAL
                     LFLCEAGAVTAAGADRGSDSRHDAEYDHRPTETRNPAAHRAQIVYQRCPDCRRARLGT
                     RDGFVEVDAGEVERYTGSAEAVVIDGPTPPALRRQVLAREGARCGNPRCYHRADHCHH
                     IVFRSHGGPTELANEVGVCRTCHALIHAGLLRVRADTHGELHWSAALTEGSPRRRVAA
                     DGAVADRLPVLHLVSDGPVRSAIVPVGAVGGPSESADADSEAASGHGLDLESLAHGLQ
                     RLGQPLARSREIIAAAARALPRGELTEAAVLRRALAMT"
     gene            complement(1636..3582)
                     /locus_tag="VD788_14205"
     CDS             complement(1636..3582)
                     /locus_tag="VD788_14205"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HXV77463.1"
                     /translation="MSLRFGAFELEVDGTELRRDGARLRMPLQALRLLELLASRAGRV
                     VTRDEIRQYLWPADTHVDFEQGINACIRQVRGALDDHADRPRYVETLPKIGYRFIQPV
                     ETVDATGDAPSRRWPRAATAVLLAVLAAGAVAAITAIGVHVAPADGPSPAAGDRRPVV
                     MVLPFRNMTGSPDDDPLGDGLTEELIAQLGRRFGASLAVIARTTAMKYRDSSLTIGQI
                     ARELDVDYLLEGSIRRGEDGLRITAQLIRGDDESHVWAANYDRGLTDPLAVQTQIGES
                     IAAALGVKLIGRDGLAAAATTPAAYEAYLRGVSALPPTGRQPIIRAREALERAVRLDP
                     GFAAAHVALARAIRIGEPGPSVWSRVRSELELALALDEELPAAHSMMGALLLYADYDR
                     EGSRDAYLRALRLNPTFAEAHHGIAAAWSTLGRHDEAIAAVHRALALDPRSESVASDV
                     GWYAYVARRYDEAIAASRLTLEIDPRYFWAHRCIVLSATLNRDLELAAAQARRHLAVL
                     AREGAPSDDARSGSGNRADPASDLDAYWRFDLAANEADVGTGPDRSDQRAVALVALGR
                     DAEAVASLERAVTRRAGWLPPFLAVDPWFDPLRPDPRFRALLARIDDAGPAGHPANRA
                     RDTGTPPSDADPSAASRTPPAIRR"
     gene            3812..>5017
                     /locus_tag="VD788_14210"
     CDS             3812..>5017
                     /locus_tag="VD788_14210"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HXV77464.1"
                     /translation="MRATPFLCSAAAAAVLLAAAPASAQFLEPDVEVLTTLEAEHLGD
                     FFGFVGEAIGDVDGDGAADYIIGAPQSGVSALFGGAAYVYSGADGTLLNVVRGGFIDF
                     LGHAVGGGGDLNGDGTPDYVIGAPNLAGFPGRVLAYSGADHSLLWQRVDPPLTFLGYD
                     VHIAGDVDGDGRSDVIAGAPLDSTVALRAGRVDVLSGVDGSPIWTTFGPQAEANLGTA
                     VGGLGDLDRDGVPEQAAGALGVVRQIGNGRTRGGAAWILDGGDGSIVRELQPNGTASA
                     FGVYFLSDAGDLDGDGTRDVFAGDYLDAQQGNVASGRAYVFSGAEDERLHSWRAENPG
                     DGFGDGRGILADVDGDGGDDVLIASYLYSEVVPGGGKAYVVSGRNGKSLRTMTSLVPG
                     ASIGVDAVAV"
BASE COUNT          747 a         1754 c         1836 g          680 t
ORIGIN      
        1 ggcaactacg tcgacgcggt gaagaccttc ctcgcgattc tgaccgaacc gatcgactga
       61 cggacgcgag ctacgtcatg gccagcgccc gacgaagcac cgcggcctcg gtgagctcgc
      121 cgcgcggcag ggcccgcgca gcggcggcga tgatctcccg cgaccgcgcc aagggttggc
      181 ccagtcgctg caggccgtga gccagcgact ccaagtcgag tccgtgccct gaagccgcct
      241 cggagtctgc atctgcagac tccgagggtc cgcccacggc gccgaccggc acgatcgccg
      301 accgaaccgg cccatccgag acgagatgca gcacgggaag ccggtccgcc acggcgccgt
      361 ccgcggccac gcgtcttctc ggggatcctt ccgtcagggc ggccgaccag tgcagctcgc
      421 cgtgcgtgtc ggcccggaca cgcaatagac cggcatggat cagggcatgg cacgtgcggc
      481 agaccccaac ctcgttggcc agctcggtcg ggccgccgtg cgaacgaaag acgatgtggt
      541 ggcaatgatc cgcccgatgg tagcagcgcg gattgccgca ccgcgcgccc tcgcgcgcga
      601 gcacctgccg gcgaagcgcg ggcggggtgg gaccgtcgat caccacggcc tcggccgagc
      661 ccgtgtagcg ctcgacctct cccgcgtcga cctcgacgaa gccgtcgcgc gtgccgagcc
      721 tcgccctgcg acagtcgggg cagcgctggt agacgatctg cgcccggtgg gccgcgggat
      781 ttcgggtctc cgtcggacgg tggtcgtact ctgcgtcgtg tcgagagtcg ctgccgcgat
      841 cggcgcccgc cgcggtgacc gcgcccgcct cgcacaggaa cagcagcgcc tgctcgagtg
      901 tgacttcttc gacgccggtg cgcgcggcga cggtggcgca ggcaccctcg atccaagtgc
      961 gcaccttctc caagtccgag cgggaaaagc gcaagacgag cttttgatcc aagttgggca
     1021 ggccgaacga cgagtcgcgc ggcttgtcgc ggccgcgtcg cagggcgtcg cgcgcctccg
     1081 cgagcgtgcg cgcgacgtcg tgctcgcgag cgaagtcgat ccacgcggat tggctggcgg
     1141 gcgaagccac ccgtgtcacc gccttcagca ccgaccagcc gatgcgtccc tggccgaagg
     1201 cctcgcgaag ctcggtcaac tcgagcagcg ctcgggccac gcgcacgcat tcgaaggtct
     1261 cggcgcggcg aagctgaaag aagcggtcgg cataggccgc cacgctcggg aagccgagtt
     1321 cgaggtggag ccgcgtctcg ccgagcacgc gcagcgcctc gcacagcgag agccgaccgc
     1381 ggttgccgat gcgaaacgcc tcgagcgcga gccggtgcac cccgtcggcg gacagcccga
     1441 agggcgacac ggccgcgggc ttcgacaacg ggatcgaggg cgggggagcg gtcgcggtac
     1501 cggacggcac cgtcgaagcg aaaggcaggc cgggcatgtg tttacctccg ggcgggtgga
     1561 aacccgagcg aggaggtggc gggcattcta gcacgaagct agggcgaaaa cgaccccgag
     1621 ctcgcgcccc gggagctacc tccgaatcgc gggcggggta cgggaggcgg cggacggatc
     1681 cgcgtcggac ggtggggtcc cggtatcgcg agcccggttg gccgggtgtc cggccggtcc
     1741 ggcgtcgtcg atccgcgcga gaagcgcgcg aaagcgcggg tccggccgca ggggatcgaa
     1801 ccacgggtcg acggcgagga acggcggcaa ccagccggcg cgccgcgtga ccgcccgttc
     1861 gagcgaggcg accgcctcgg cgtctcgacc gagcgcgacc agcgcgacgg cgcgctggtc
     1921 cgaacggtcg ggacccgttc cgacatcggc ctcgttcgcc gcgaggtcga atcgccagta
     1981 ggcgtcgagg tccgaggcgg gatccgcgcg gttgccggat ccggaccgcg cgtcgtcgga
     2041 gggagcgccc tcccgcgcca gcacggcgag gtgccgccgc gcttgagcgg ccgccagctc
     2101 gagatctcgg ttcaacgtcg cggagaggac gatgcagcga tgcgcccaga aatagcgcgg
     2161 atcgatctcg agcgtcagcc gcgaagcggc gatcgcctcg tcgtagcgcc gggcgacgta
     2221 ggcgtaccaa ccgacgtccg aggccaccga ctccgaccgc gggtcgagcg ccagcgcccg
     2281 gtggacggcg gcgatcgcct cgtcatgccg cccgagcgtg gaccacgccg cggcgatgcc
     2341 gtggtgcgcc tcggcgaacg tcgggttgag ccgcaacgcg cgcagatagg cgtcgcgcga
     2401 accctcgcgg tcgtagtcgg cgtacagcag cagcgctccc atcatcgaat gcgccgcggg
     2461 tagctcctcg tcgagcgcga gcgccagctc gagctcgctc cgtacgcgcg accagaccga
     2521 cggtccgggc tcgccgatgc ggatcgcacg ggccagcgcg acgtgcgcgg cggcgaagcc
     2581 ggggtcgagg cgcaccgcgc gttcgagcgc ttcccgcgcc cggatgatcg gctggcgtcc
     2641 cgtggggggc agcgccgaaa ccccgcgcag ataggcttcg taggccgcgg gcgtggtcgc
     2701 cgcggcggcg agaccgtcgc gaccgatcag cttcacgccg agcgcggccg cgatgctctc
     2761 gccgatctgg gtttgcacgg cgagcgggtc ggtgaggcct cgatcgtagt tggcggccca
     2821 gacgtgggat tcgtcgtcgc cgcggatcag ctgcgcggtg atgcgcagcc cgtcttcgcc
     2881 gcggcggatg ctcccttcga gcaggtagtc cacgtcgagc tcgcgggcga tctgaccgat
     2941 ggtcagcgac gaatcgcgat acttcatcgc ggtcgtacga gcgatcacgg ccaacgacgc
     3001 gccgaagcgc cgcccgagct gtgcgatcaa ctcctcggtc aacccgtcgc cgagcggatc
     3061 gtcgtcggga gagcccgtca tgttgcggaa cgggagcacc atcacgaccg ggcgtcgatc
     3121 ccccgccgcg ggtgagggcc catcggccgg cgccacgtgc accccgatcg cggtgatcgc
     3181 cgccaccgcg cccgcggcga gcacggcgag aagcaccgcc gtcgcggccc tcggccagcg
     3241 tcgcgacggg gcgtcgccgg tcgcgtcgac cgtctcgacc ggctggatga accggtagcc
     3301 gatcttgggc aaggtctcga cgtaacgcgg ccggtcggcg tggtcgtcga gtgcaccacg
     3361 aacctggcgg atgcacgcgt tgatcccttg ctcgaagtcg acgtgggtat ccgcgggcca
     3421 caggtactgg cggatctcgt cgcgtgtgac cacccgaccc gcgcgcgacg ccagcagctc
     3481 gagcaggcgc agggcctgga gcggcatccg caaccgggcc ccgtcccggc gcaactcggt
     3541 gccgtcgacc tccagctcga acgcgccgaa ccggagcgac acccgcgggt cccccgtcat
     3601 gtcgctcgta tacggtgacg cgcggcgaaa cctacacggt cggtcgggca tgctgcggtg
     3661 aatggaccgc agcgtcggct cgcgggtttc agcggatttt catctctttc tcaggctccg
     3721 tccatgggcc cggcggagcg cgcggcgtac cgtccgtccc gcgaatccat cgacccactc
     3781 cggggctgcc ccgggaggaa aggagaacgt gatgagagcc acaccgtttc tttgctcggc
     3841 ggccgcggcg gccgtcttgc tggccgccgc gccggcctcc gcgcagttcc tcgaaccgga
     3901 cgtcgaggtc ctgaccacgc tcgaagcgga gcacctgggc gacttcttcg gcttcgtcgg
     3961 cgaggcgatc ggcgacgtcg acggcgacgg tgccgccgac tacatcatcg gcgcaccgca
     4021 gagcggcgta tcggccctgt tcggcggagc ggcctacgtg tactcgggag ccgatggcac
     4081 gctgctcaac gtcgtccgcg gcggcttcat cgacttcctc ggtcacgccg tcggcggtgg
     4141 cggcgacctg aacggcgacg ggacgccgga ctacgtgatc ggcgcgccca acctggccgg
     4201 gtttcccggg cgggtgctgg cctactccgg cgcggaccac agcctgctgt ggcagcgcgt
     4261 cgatcctccg ctcacgttcc tcggctacga cgtccacatc gcgggcgacg tcgatgggga
     4321 cgggcggtcc gacgtcatcg ccggagcgcc tctcgattcg acggtcgcgc tgcgcgccgg
     4381 gcgcgtcgac gtcctgtcgg gcgtcgacgg ctcgccgatc tggaccacgt tcggaccgca
     4441 ggccgaggcc aacctcggca cggccgtcgg cggcctcggt gatctcgatc gggacggagt
     4501 gcccgagcag gcggccggtg ccctgggagt ggtgcggcag atcggcaacg gtcgcacgcg
     4561 gggcggcgcg gcgtggattc tcgacggcgg cgacggctcg atcgtcaggg agctgcagcc
     4621 gaacggcacg gcgagcgcgt tcggcgtcta cttcctgagc gacgcgggcg atctcgacgg
     4681 cgacgggaca cgcgacgtct tcgccggcga ctatctcgat gcccagcagg ggaacgtcgc
     4741 cagcggccgg gcctacgtct tctccggtgc cgaggacgaa cggttgcaca gttggcgcgc
     4801 ggagaacccg ggcgacggct tcggcgacgg acgaggcatc ctcgccgacg tcgacgggga
     4861 cggcggcgac gacgtgctga tcgcatccta tctctacagc gaggtcgtcc caggcggtgg
     4921 caaggcctac gtcgtgtcgg gccgaaacgg caagagcctg cgcacgatga ccagtctcgt
     4981 gcccggggcc agcatcggcg tcgacgcggt ggccgtc
//
LOCUS       DASPGN010000488         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_207495, whole genome shotgun sequence.
ACCESSION   DASPGN010000488 DASPGN010000000
VERSION     DASPGN010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207495"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VD788_14215"
     CDS             <1..312
                     /locus_tag="VD788_14215"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HXV77465.1"
                     /translation="RVQSARRTQHEQPEVGVDFGHDRFRADLERLAAGVGCLAGGSRG
                     SVPPFGPPITGLELYVDESILRNDRVAFNAGSLTDSILMERSTYVEIAKPVEIFSFSR
                     P"
     gene            406..3162
                     /locus_tag="VD788_14220"
     CDS             406..3162
                     /locus_tag="VD788_14220"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXV77466.1"
                     /translation="MTCYPRARLLGGVALLIIATQPLVLAASSLGDEIVIPYERFVLD
                     NGLTLIVHEDHKAPIVAVNVWYHVGSKNEPDGRSGFAHLFEHLMFNGSEHYNDDYFQV
                     MERLGSTDLNGTTNEDRTNYFQNVPTSALDTVLWLESDRMGHLLGAVDQERLDEQRGV
                     VQNEKRQGDNQPYAKFWEMMTHATYPEGHPYSHTVIGSLDDLDAAELDDVKEWFQTYY
                     GAANAVVVVAGDVDAETAKSRVEHYFGDIPAGPPIGKFDAWVAQMEGTRRQSAYDRVP
                     QARLYKVWNVPPFGSDDQIFLQIASSVLASGKTSRLYNRLVYRDQIATDVSSFIDDRE
                     IGSQFHVWATAKPGQDLTDVERAVDEELRRFVRDGPSEAEVERARTETIAGYIRGLER
                     IGGFGGKSDVLAQMEVFLGDAGAYRAYLDTMRNANAAKIKAAVQRWLDDGVYIFEMHP
                     FPDFKTAENGADRSKLPEPGTPPAAKFPEIEQVTLSNGVTVLLAERHAIPVVDVTLLV
                     DAGYAADQFAIPGTASLTLNMLDEGTQSKDALEISEAEQTLGAEIDTGSGLDTSFVGL
                     TAMKANLDASLALFADVVLNPSFPAQELERLKKMQIAGIQREKSTPIQMALRIFPKLM
                     YGDGHAYGNPFTGSGDIDGVTKMTRDDLIEFHQTWFKPNNSTIIVVGDTTIEEMTPKL
                     ESLFKRWKASDVPQKNIGAVTLKEKQTIYVIDRPGSPQSVILAGHVSPPKNNPDEIAI
                     ESMNQVLGGTFTSRVNMNLREDKHWSYGSRTIIVDAKGQRPFIAFAPVQSDKTAESMQ
                     ELAKELSEILDDRPIEQGELDKVQANRILRLPGSWETSGEVASAIREIVEFGLPLDYY
                     NSFAAEVRALQLADVQQAAERVVKPDGVTWVVVGDRSQIEQAIRGLGWADVKLLDADG
                     NPVN"
     gene            complement(3175..>3757)
                     /locus_tag="VD788_14225"
     CDS             complement(3175..>3757)
                     /locus_tag="VD788_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77467.1"
                     /translation="VTLPVSLLNLAGEASGQAVGALYSSIFGRAAVRQGSAAYASIAP
                     GEVGASLSPHFSVTIDPRTSCGSRLPFRLRAAAAGAFGDSSFLLEIGAREADGVTIAC
                     EPFSCPDAALASGIAGAMRVAPSGDDLRFSWPDVPDSTVYELWHSVDRDFSTAALLGR
                     FESTSHVVVGAMTAPENGFYRVRAVNDCGWATD"
BASE COUNT          737 a         1243 c         1213 g          564 t
ORIGIN      
        1 cgcgtccagt cggcgcgccg cactcaacac gaacagccgg aagtcggcgt cgactttggc
       61 cacgatcgct ttcgcgccga tctcgagcga ctcgccgcgg gcgtcggctg cctcgcggga
      121 ggttcgcgtg ggagcgtgcc gccgttcggg ccgccgatca ccgggttgga actgtacgtc
      181 gacgaatcga tcctgcgtaa cgaccgggtt gcgttcaacg ccggatcgct gaccgactcg
      241 atcctgatgg aacgctcgac ctacgtcgaa atcgccaaac cggtcgaaat cttctccttc
      301 tcccgaccgt aaggagaccg ggggcgcgcg atcggccgga agtccggtag aatcccctcg
      361 cacgttatcc cgaacgccct gtttttcagt cgtaggaggc gacccatgac ctgttaccct
      421 cgagcgcgct tgctcggcgg cgtcgcgttg ctgatcatcg cgacccagcc gttggtcctc
      481 gccgcttcga gtctcggcga tgagatcgtg atcccgtacg agaggttcgt gctcgacaac
      541 ggcctgacgc tgatcgtcca cgaagaccac aaagcgccga tcgtcgcggt caacgtctgg
      601 taccacgtcg gatcgaagaa cgagccggac ggccgcagcg gtttcgccca cctgttcgaa
      661 cacctgatgt tcaacggcag cgagcactac aacgacgact acttccaggt gatggagcga
      721 ctcggttcga ccgatctcaa cggtacgacc aacgaagacc gcaccaacta tttccagaac
      781 gtaccgacct cggcgctcga tacggtgctg tggctcgaat cggaccggat gggccaccta
      841 ctcggcgccg tcgaccagga acgtctcgac gagcaacggg gtgtggtgca gaacgagaag
      901 cggcagggcg acaaccagcc gtacgccaag ttctgggaga tgatgaccca tgcgacctat
      961 cccgaagggc atccctactc ccacaccgtg atcgggtcgc tcgacgacct cgacgcggcc
     1021 gagctcgacg acgtcaaaga gtggttccag acctactacg gcgccgccaa tgccgtagtg
     1081 gtcgtcgccg gcgacgtcga tgccgaaacc gccaagagca gggtcgagca ctacttcggt
     1141 gacattcccg ccgggccgcc gatcggcaag ttcgacgcgt gggtcgccca gatggaaggc
     1201 acgcgccgcc agagcgccta cgatcgcgtg ccgcaggcca ggctctacaa agtctggaac
     1261 gtgccgccgt tcggcagcga cgaccagatc ttcctccaga tcgcctcatc ggtcctcgcc
     1321 tcgggcaaga cgtcacggct ctacaatcgg ctggtctacc gcgaccagat cgcgacggac
     1381 gtctcgtcgt tcatcgacga ccgcgagatc ggcagccagt tccacgtgtg ggcgacggcc
     1441 aagccggggc aagatctgac cgacgtcgaa cgggcggtcg acgaagagct ccggcgcttc
     1501 gtccgcgacg gcccgagcga ggcggaggtc gaacgggcac gcaccgagac gatcgcgggt
     1561 tacatccgcg gcctggagcg catcggcggg ttcggcggca agtccgacgt gctggcgcag
     1621 atggaggtct tcctcggcga cgccggcgcg taccgcgcct acctcgacac gatgcggaac
     1681 gcgaacgcgg ccaagatcaa agcggccgtc caacgctggc tcgacgacgg cgtttacatc
     1741 ttcgagatgc atccgttccc ggacttcaag acggccgaga acggcgccga ccgcagcaag
     1801 ctgcccgagc ccggaacgcc gccggccgcc aagttccccg agatcgaaca ggtcacgctg
     1861 tccaacggcg tcaccgtcct gctcgccgag cgacacgcga tcccggtggt cgacgtaacg
     1921 ctgctggtcg atgcgggcta cgccgccgat cagttcgcca tcccgggcac cgccagtctg
     1981 acgctgaaca tgctggacga aggcactcaa tcgaaggacg cactcgagat cagcgaggcc
     2041 gagcagacgc tgggcgccga gatcgacacg gggtccggcc tcgacacgtc gttcgtcggg
     2101 ctgaccgcga tgaaggccaa tctcgacgcc tcgctggcgt tgttcgccga cgtcgtgctc
     2161 aacccgtcgt tccccgccca ggagctcgaa cgtctcaaga agatgcagat tgcggggatc
     2221 cagcgcgaga agtcgacgcc gatccagatg gcactgcgca tcttccccaa gctgatgtac
     2281 ggcgacggac acgcctacgg aaacccgttc accggttcgg gtgacatcga cggcgtcacg
     2341 aagatgaccc gtgacgacct gatcgaattc caccagacct ggttcaagcc gaacaactcg
     2401 acgatcatcg tcgtcggtga cacgacgatc gaggagatga cgcccaagct cgagtcgttg
     2461 ttcaagcgct ggaaagcgag cgacgtgccg cagaagaaca tcggcgccgt cacgctgaag
     2521 gagaaacaga cgatttacgt catcgaccgg cccggctcgc cgcaatcggt gatcctcgcc
     2581 ggccacgtct caccaccgaa gaacaacccg gacgagatcg cgatcgagtc gatgaatcag
     2641 gtgctgggcg ggacgttcac gtcgcgggtc aacatgaatc tgcgggagga caaacactgg
     2701 tcgtacggtt cgcgaacgat catcgtcgat gccaagggcc agcgtccgtt catcgccttc
     2761 gcgccggtgc agagcgacaa gacggccgaa tcgatgcagg agctggcgaa ggagttgagc
     2821 gagatcctcg acgatcgccc gatcgagcag ggcgagctcg acaaggtgca agccaaccga
     2881 atcctccggc ttcccgggtc gtgggagacg tcgggcgagg tcgcgtccgc gatccgcgag
     2941 atcgtcgagt tcggactgcc gctcgactac tacaacagtt tcgccgccga ggtccgagcg
     3001 ctgcagctgg ccgacgtgca gcaggcggcc gagcgcgtcg tgaagcccga cggtgtgacg
     3061 tgggtcgtcg tcggcgatcg ctcgcagatc gaacaggcga tccgcggtct cggctgggcc
     3121 gacgtgaagc tgctcgacgc agacggcaac cccgtcaact gagccgggcg tccgctagtc
     3181 ggtcgcccaa ccgcaatcgt tgacggcgcg aacgcggtag aagccgtttt cgggcgcggt
     3241 catcgccccg acgacgacgt gagaggtcga ctcgaaacga ccgagcagcg cggcggtgct
     3301 gaagtcgcgg tcgaccgaat gccacagctc gtagacggtg gaatccggaa cgtcgggcca
     3361 gctaaaccgc aggtcgtcgc ccgacggcgc gactcgcatc gctccggcga tgcccgatgc
     3421 gagcgcggcg tcggggcacg agaacggctc gcaggcgatc gtcaccccgt ccgcctcgcg
     3481 cgcgccgatc tcgaggagaa aggacgaatc gccgaacgca ccggccgctg ccgcgcggag
     3541 acggaacggc agccggctgc cgcacgacgt ccgcggatcg atcgtcacgc tgaagtgcgg
     3601 cgagagcgaa gcgccgacct cccccggagc gatcgaagcg tatgcggccg acccctgccg
     3661 aaccgcggct cgaccgaaga tgctgctgta gagcgcgccg accgcctggc cggaagcctc
     3721 gccggcgaga ttgagcagtg agaccggcaa cgtgacg
//
LOCUS       DASPGN010000489         6453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_442361, whole genome shotgun sequence.
ACCESSION   DASPGN010000489 DASPGN010000000
VERSION     DASPGN010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442361"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..379
                     /locus_tag="VD788_14230"
     CDS             119..379
                     /locus_tag="VD788_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77468.1"
                     /translation="MGTLPPGNYDQLVVVMTDVEYVLEDGTKVKITPPGGGWTSIVPV
                     CPFEVVEGAMTTIDLRFSPRDAFREENGQMRFEPAFRCETGD"
     gene            complement(412..1281)
                     /locus_tag="VD788_14235"
     CDS             complement(412..1281)
                     /locus_tag="VD788_14235"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV77469.1"
                     /translation="MRHVLNRGLRKVRYEIVKLPRDRDPRAEYAALYPAQSLDGRRFY
                     NVGAGGFSHPLWTNVDFISDWYSRNDDNTRAGIQYDLFSLEPIPVEDATAEVVYTSHT
                     VEHIHDEAAANAFREAYRMLKPGGFFRVTTPDAELAHAALERGDRAYFWWMFDPRRNT
                     GGNLVKPLTEASIEQVFLVLIATSVSTIVDRGGDERVDDAKLRELLRRLGRDRTLEYC
                     TSRCPVEIQQAFPGYHMNWWTAEKLSRMLREAGFDTTYRSGYGQSHCPVLRDVTHFDN
                     TRPYMSLYVEAVK"
     gene            1370..2116
                     /locus_tag="VD788_14240"
     CDS             1370..2116
                     /locus_tag="VD788_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77470.1"
                     /translation="MKRLTAEEFRTRKRSDTLVIYGSGFSIRQLTPEDRENLARFDSI
                     SFNWFCKSNIPTTFYTIREQATLPIRIRPGEEVEDLLETLNGPAYRDTCLIVLDMTGH
                     TPEAFPWAADANLDRLHGEGVVLTDLRKGKPSDFRDDIFEVGVHHGKNSMTTQLHLAV
                     YLGYRSIVFVGVDLYDCRYFWLGEDEDRVSRDSRNLTRHSRHPTTDATLKAIKATRRR
                     YRIEMYSFNPKSLLTEVLPAGLPNREPITR"
     gene            2121..4244
                     /locus_tag="VD788_14245"
     CDS             2121..4244
                     /locus_tag="VD788_14245"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXV77471.1"
                     /translation="MRPLRIYLGDLTYTTVSLANEAFPLNIGYVAAYARETHGDSVEI
                     ELFKYPDELERAVRTTPPDILGLSNYPWNHSLDLEFFRLAHRLRADTLCVMGGSNIPH
                     EADRQQAFLEQEPEIDIYVYLEGEVGFSNVVQRVLDSGSTGARGEIRSAPIDGCLFVD
                     DDGRFVRGTVIPRSRSLDEYPSPYLTGILDKFFDGKLSPMMESNRGCPFSCTFCHEGH
                     DVYRKVNHFSLDRVKAELEYIADRVTGPVHNLMFCDPNFGMYSRDLEICKTIARIQAE
                     RGWPRDIFATTGKNKKDRIAESLSILNGTMQMWISVQSMDETVLKNIKRENISLEDMM
                     NIQSSLTANYLPSKSEIILGLPGETYDSHIADIGKLVTAGVDTITPYTLMLLHGTEMN
                     TPAEREKWGFKTRYRVLPRDFGRMGDGRTVVEAEEVVVGTKDLSEDEYVALRRFHLII
                     ATIYNGKPFAALFRLLKERRIDVFPLLKAILENSRRAPSIVRRRIEEFERETRGELWN
                     SDAEMREFYADDKNFERLVTGELGANLLQKYVALSLIDTDAWARYVFEIAAEYLAADG
                     ASADTVEALDDIRRYCTARVHNLFGPDRLEDSPRATLRHDIEAWCHHANTRPLADFRF
                     DAPREMIFTFSEEQHRTTDDYIERFGRSHQGIGKMLTKMNIMNAWRRCVPPDRTTMKD
                     RDEREVYYALIDENASGGVIARHDQ"
     gene            4674..6152
                     /locus_tag="VD788_14250"
     CDS             4674..6152
                     /locus_tag="VD788_14250"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HXV77472.1"
                     /translation="MTRSLALVFYLLAGLVAPSTSAQDYRVERDLLGEKKIPAEAYYG
                     VQTARALENFQLSGIAINHYPGFVEAWAIVKLAAARANTDVGAMKPEVLAGIEKAAKA
                     VRDGKYHDQFLVDWYQGGAGTSTNMNANEVLANVALELSGHRKGEYEIIEPHDDLNMS
                     QSTNDSYPTAIKVALLLRNDRLIAELEKLSASFRSKGDAFLMVVKMGRTELQDAVPMT
                     VGQEFHAFAASLDAEIALLRDVERYLYPVNMGATAIGTGINVPKGYPERCAKHLADLT
                     GKPIVPSTDMIAATWDQQAFVVYASALKSVAVKISKIASDLILLTSGPRAGLAEINLP
                     ALQPGSSIMPGKVNPVVPEVMNIIAFRVMGNDYAVTLAAHSGQLQLNAYEPLAGLASM
                     ESQHLLHHGSMLFREKCIDGITVNEQVLAHYMTTTVGIVTALNPVLGYEKATELAAEA
                     YESGKGILEIIREKKVLTEEQIAELLDPVKLTNLDPAIYQKK"
     gene            6189..>6453
                     /locus_tag="VD788_14255"
     CDS             6189..>6453
                     /locus_tag="VD788_14255"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HXV77473.1"
                     /translation="MKVRRIAALLAVSLLVGCSGTAQSADKPTVRILATGGTIAGAAR
                     ESTDAGYKSGAVGVDVLIEAVPQMKEIADVSGEQIASIGSQDMN"
BASE COUNT         1234 a         2119 c         2041 g         1059 t
ORIGIN      
        1 gcccgcggcg ccgccgatct cgactgcacg attggatgat catgctcgct accgccagcc
       61 gaactcaatc agccccggtt actcggacag gctcctaggc ggatcgatcg atcttccgat
      121 gggcactttg ccccccggca actacgatca gctcgtggtc gtgatgaccg acgtcgaata
      181 cgtcctcgag gacgggacga aggtcaagat caccccgccc gggggcggat ggacgtcgat
      241 cgtcccggtc tgtccattcg aggtcgtcga gggtgcgatg acgacgatcg acctgcgttt
      301 cagcccgcgt gacgccttcc gggaggagaa tggccagatg cggttcgagc ccgctttccg
      361 ctgcgaaacc ggcgactgaa tcgaccgctc gacctcacgc cggtcgaggt gctacttgac
      421 ggcctcgacg tacagcgaca tgtagggacg cgtgttgtcg aagtgggtca cgtcccgcag
      481 gaccggacag tggctctgac cgtaaccgga tcggtaggtg gtgtcgaatc ccgcctcgcg
      541 caacatgcga ctcagtttct ctgcggtcca ccagttcatg tggtagccgg gaaacgcctg
      601 ctggatctcg accgggcagc ggctcgtgca gtactcgagc gtccgatcgc gccccagcct
      661 gcggagcagc tcgcggagct tcgcgtcgtc gactcgctcg tcgcccccgc gatcgacgat
      721 cgtcgagacg ctggtcgcga tcagcaccag gaacacctgc tcgatcgagg cctccgtcag
      781 cggtttgact agattgccgc cggtgttgcg ccgcggatcg aacatccacc agaagtatgc
      841 gcgatctccg cgctcgagcg ccgcgtgagc cagctcggcg tcgggtgtcg tcacccggaa
      901 gaaaccgccc ggcttcaaca tacggtaggc ctcgcggaac gcgttcgccg cggcctcgtc
      961 gtggatgtgc tcgacggtgt ggctggtgta gacgacctct gcggtggcgt cctcgacggg
     1021 gatcggctcc agcgagaaca ggtcgtactg gatacccgct cgggtgttgt cgtcgttgcg
     1081 cgagtaccaa tccgagatga agtcgacgtt ggtccacagc gggtgcgaga atccgcccgc
     1141 cccgacgttg tagaaccgcc ggccgtcgag gctctgtgcc gggtacagcg ccgcgtactc
     1201 cgcgcgcggg tcgcgatcgc gcggcagctt gacgatctcg tagcggacct tgcgcagtcc
     1261 ccggttcagg acgtgcctca cctgtcctcc tcgcaacgga cggcggaatg gtatcttatg
     1321 gggcgatgcc ggtgcacgtc cgtagcggag gcaacgacgg ggacgatcga tgaagcgact
     1381 gaccgcggag gagttccgca cccgcaaacg atcggacacg ctggtgatct acggttcggg
     1441 cttctcgatc cgccagctca cgcctgagga ccgcgagaac ctcgcgcgat tcgacagcat
     1501 cagcttcaac tggttttgca agtcgaacat ccccacgacg ttctacacga tccgcgagca
     1561 ggccaccctg ccgatccgca tccgtcccgg cgaggaggtc gaagacctgc tcgagacgct
     1621 gaacgggccg gcgtatcgcg acacctgcct gatcgtgctc gacatgaccg ggcacacgcc
     1681 ggaggccttc ccgtgggccg ccgacgccaa cctcgaccgg ctgcacggag agggtgtcgt
     1741 gctgaccgac ctgcgcaagg gcaagccgtc ggatttccgt gacgacatct tcgaggtcgg
     1801 cgtgcaccac ggcaagaact cgatgaccac gcagttgcat ctggcggtct acctcggcta
     1861 ccgctcgatc gtcttcgtcg gcgtcgatct gtacgactgc cgctacttct ggctcggcga
     1921 ggacgaggac cgcgtcagcc gcgactcgcg caatctgacc cgtcacagcc gccatccgac
     1981 gaccgatgcg acgttgaagg cgatcaaggc gacgcgccgg cgctatcgta tcgagatgta
     2041 cagcttcaac ccgaaatcgc tgctgaccga ggtcctgccg gccggccttc ccaatcgcga
     2101 accaatcacg aggtgagtcg atgcgcccac tcaggatcta cctcggcgat ctcacgtaca
     2161 cgacggtcag cctggcgaac gaggcattcc cgctgaacat cggctacgtc gcggcctacg
     2221 cccgcgagac ccacggcgac tccgtcgaaa tcgagctgtt caagtatccc gacgaactcg
     2281 aacgcgcggt gcggacgacc ccgccggaca tcctcgggct cagcaactac ccgtggaacc
     2341 actcgctgga cctcgagttc ttccggctgg cgcaccggct ccgcgccgat acgctgtgcg
     2401 taatgggcgg gtcgaacatc ccgcacgaag ccgatcgcca gcaggccttt ctcgagcaag
     2461 aacccgagat cgacatctac gtctacctcg agggggaagt cggtttcagc aacgtcgtcc
     2521 agcgagtgct cgacagcggg agcaccggcg cgcgcggcga gatccggtcc gcgccgatcg
     2581 acgggtgcct gttcgtcgac gacgacgggc gattcgtccg cgggaccgtc atcccgcgca
     2641 gccgcagtct cgacgagtac ccgtcaccct acctgaccgg catcctcgac aagttcttcg
     2701 acggcaagct gagcccgatg atggagtcga atcgcggctg cccgttcagc tgcaccttct
     2761 gccacgaggg acacgacgtc tatcgcaagg tcaaccactt cagcctcgac cgcgtcaagg
     2821 ccgagttgga gtacatcgcg gaccgcgtaa cgggaccggt acacaacctg atgttctgcg
     2881 accccaactt cggcatgtac agtcgcgacc tcgaaatctg caagacgatc gccaggatcc
     2941 aggccgaacg cggctggccg cgtgacatct tcgccaccac cggcaagaac aagaaagacc
     3001 ggatcgccga atcgctgtcg atcctcaacg gcacgatgca gatgtggatc tcggtgcagt
     3061 cgatggacga gacggtgctg aagaacatca agcgcgagaa catcagcctc gaggacatga
     3121 tgaacatcca gtcctcgttg accgcgaact acctgccgtc caagtccgag atcatcctcg
     3181 gactccccgg cgagacctac gacagccaca tcgccgacat cggcaagctg gtgaccgccg
     3241 gcgtcgacac gatcacgccg tacacgctga tgctgctgca cggcaccgag atgaacacgc
     3301 cggcggagcg cgagaagtgg gggttcaaga cccggtaccg ggtactgccg cgcgacttcg
     3361 gccggatggg cgacggccgt acggtggtcg aggccgagga ggtcgtcgtc ggcaccaagg
     3421 atctcagcga ggacgagtac gtcgcgctgc gtcgcttcca cctgatcatc gccacgatct
     3481 acaacggcaa accgttcgcc gcgctgttcc gtctgctgaa ggaaaggcgg atcgacgttt
     3541 tcccgctgct gaaggcgatt ctggagaact cgcgccgggc accgtcgatc gtgcgccggc
     3601 gtatcgagga attcgagcgc gagacgcggg gcgagctgtg gaactcggac gcggagatgc
     3661 gcgagtttta cgccgacgac aaaaacttcg agcgactggt caccggagag ctcggcgcca
     3721 acctgttgca gaagtacgtc gcgctgtcgc tgatcgacac cgatgcctgg gcgcgctacg
     3781 tcttcgagat cgcggccgag tacctcgccg ccgacggcgc ttcggccgac accgtcgagg
     3841 cgctggacga catccggcgc tactgcacgg ctcgcgtgca caacctgttt ggacccgatc
     3901 ggctcgagga cagcccgcgc gcgacgctgc gccacgacat cgaagcctgg tgccatcacg
     3961 ccaacacccg gccgctcgcg gactttcgct tcgacgcacc gcgcgagatg atcttcacct
     4021 tcagcgagga acagcatcgc accaccgacg actacatcga gcgcttcggg cgcagccacc
     4081 agggcatcgg caagatgttg accaagatga acatcatgaa cgcgtggaga aggtgtgtgc
     4141 cgcccgaccg gacgacgatg aaggatcgcg acgaacgcga ggtgtactac gcgttgatcg
     4201 acgagaacgc ctcgggcggc gtgatcgccc gtcacgacca atgaacggcg gcgacccggc
     4261 acgttgcccg cttcgacaac gaggccttcg aagaaggcac cgacgtacca agtactgccg
     4321 ccgttgcgtg gaactccaac cgcgttccgg cttcgcttga gtccgagatg agtgattacg
     4381 tcgactcgaa ccgccggcaa ccggcccgcg cgacggggga cggagtccca atctgagtcc
     4441 aatccaccga cgttgcgtgg gtcggttgtc tcgggcggtc cttgcgtcag agccccaacc
     4501 gacccgaccc ccaattgtaa cgggggcgcg gagcgcgcgg cgagccgggg gtatttgcac
     4561 ccgaacctgg tagtttttcc aggttgtcgc accgccctgg tcggttacga tgcctccgga
     4621 gctgagcgag cgcacagcgg attcggtgcg ctcctgcgct agcggaggtt gtcatgacac
     4681 gcagtctcgc cctcgtattc taccttctcg ccgggctcgt ggcgccatcg acgtcggctc
     4741 aggactaccg cgtcgagcgg gatctgctcg gcgaaaagaa gattccagcg gaggcctact
     4801 acggtgtgca gacagctcgc gcgctcgaga acttccagct ctccggcatc gcgatcaacc
     4861 actaccccgg cttcgtcgag gcctgggcga tcgtcaagct ggccgcggcg cgcgccaaca
     4921 ccgacgtcgg cgcgatgaag cccgaggtgc tcgcgggcat cgagaaggcg gccaaggccg
     4981 tccgcgacgg caagtaccac gaccagttcc tggtcgactg gtaccagggc ggcgccggga
     5041 cctcgacgaa catgaacgcc aacgaggtgc tagccaacgt cgcgctcgag ctttccgggc
     5101 atcgcaaggg cgagtacgag atcatcgagc cgcacgacga tctcaacatg tcgcagtcga
     5161 cgaacgactc gtacccgacc gcgatcaagg tggcgctgct tttgcgcaac gaccgactga
     5221 tcgcggagct cgagaagctg tcggcctcgt tccggtccaa gggcgatgcg ttcctgatgg
     5281 tggtaaagat gggacgcacc gagttgcagg acgccgtgcc gatgacggtg ggacaggagt
     5341 ttcatgcgtt cgcggcatcg ctcgacgccg agatcgcgct gctgcgcgac gtcgaacggt
     5401 atctctaccc ggtgaacatg ggcgccaccg ccatcggcac ggggatcaac gtgccgaagg
     5461 gctatcccga gcggtgcgcg aagcacttgg cggatctcac gggcaagccg atcgtgccat
     5521 ccaccgacat gatcgccgcg acgtgggacc agcaggcgtt cgtcgtctac gcctccgctc
     5581 tcaagagtgt ggcggtcaag atctcgaaga tcgcgagcga cctcatcctg ctcacgtcgg
     5641 ggccgcgggc cggcctggcc gagatcaacc taccggcgct ccagccgggc tcctcgatca
     5701 tgcccggcaa ggtcaacccg gtggtacccg aggtgatgaa catcatcgcc ttccgcgtga
     5761 tgggcaatga ctatgccgtg accctggcgg cgcactccgg ccagcttcag ctcaacgcct
     5821 acgagcctct ggcgggcctg gcgtcgatgg agtcgcagca cctgctccac cacggctcga
     5881 tgctcttccg agaaaagtgc atcgacggca tcaccgtcaa cgagcaggtg ctcgcgcact
     5941 acatgacgac gacggtcggc atcgtgaccg cgctgaaccc ggtcctcggc tacgagaagg
     6001 cgaccgagct cgcggccgag gcctacgaaa gcggcaaggg gatcctcgag atcatccgcg
     6061 aaaagaaggt cctgacggaa gaacaaatcg ccgaactgct ggatccggtg aaactgacca
     6121 atctggatcc tgcgatctac caaaagaagt agcgacgagg cccctcgtcg aacacaggag
     6181 ggatgtccat gaaggtacga cgcatcgctg ctcttctcgc ggtttcgctc ctcgtcggat
     6241 gctcgggaac cgcccagagc gcggacaagc ccacggtcag gatcctggcc accggcggga
     6301 cgatcgcggg cgccgcccgg gagtccaccg atgcaggcta caagtcgggc gcggtcggtg
     6361 tcgacgtgct gatcgaggcg gtgccccaga tgaaggagat cgccgacgtc agcggggagc
     6421 agatcgcctc catcggaagc caggacatga acg
//
LOCUS       DASPGN010000490         6699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_91122, whole genome shotgun sequence.
ACCESSION   DASPGN010000490 DASPGN010000000
VERSION     DASPGN010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6699
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91122"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VD788_14260"
     CDS             <1..703
                     /locus_tag="VD788_14260"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXV77474.1"
                     /translation="GVIQPLEEVGRACRDRGIPFVVDAVQAAGKLPVDPKPVFADLLA
                     VSSHKLGGPQGAGALIVRSGIELARLIGGGAQEKRRRGGTEPVVAIAGFGAAARAALG
                     GMKRDNARMLHLRAKIETRLRELFPDLRFHGQDAPRLPNTVNFALPDVPGEMLVIAAD
                     LAGIALSTGSACASGAVEPSHVILALGRSASEARNAVRLSVGWSTTHEEVDLFLERFP
                     GVVRRVRDGLKGGAD"
     gene            complement(700..1077)
                     /locus_tag="VD788_14265"
     CDS             complement(700..1077)
                     /locus_tag="VD788_14265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV77475.1"
                     /translation="MSQPKRILVVDDDAPVVELLREVLVDQDYEVDTASTGSQALERI
                     RAEIYDAAIVDFGLPDMDGVTLHHRIRQLDDELAGHTIFISGMSQTEDNLGYFAAYSR
                     GFLSKPFEVREVLAAVRGIFAAD"
     gene            1246..2307
                     /gene="mnmA"
                     /locus_tag="VD788_14270"
     CDS             1246..2307
                     /gene="mnmA"
                     /locus_tag="VD788_14270"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXV77476.1"
                     /translation="MSGGVDSSVAASLLARDGRSVVGFSMQLVDRLAGEQERYGRCCS
                     PDDFRDARRVADRYRFPHYILDMEREFERLVLRPFADDYATGRTPSPCVRCNTFIKFG
                     ALLSRARAVGADRVATGHYAILEPDRRTGRTLLRRAVDRDKDQSYFLFDLSDEQRQAA
                     EFPLGRLTKDRVRQLAAEMGLAAADKPESMDLCFVARDESYRDFLARNRLAPAAAPGE
                     VVDGTGRVLGWHDGVSRFTVGQRRGLGISAAERLYVIGVDPSTRRVEVGGDAALYRDR
                     CVIERTRWIAFERPGGALRASVRIRSTHEGAAASLRELGDGRAEIRFDQPQRAITVGQ
                     AAVAYDGDLVLGGGWIAAA"
     gene            2404..5277
                     /gene="gcvP"
                     /locus_tag="VD788_14275"
     CDS             2404..5277
                     /gene="gcvP"
                     /locus_tag="VD788_14275"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HXV77477.1"
                     /translation="MKNRSPVDPTDLFAPRHIGSAPDQLQAMLGSLGFDSLDRLSDAI
                     VPDDIRLRRDLRLGPPRSESRALAELRQIARHNEVYRSFIGLGYHGCITPPVIQRNIL
                     ENPGWYTQYTPYQAEIAQGRLEALLNFQTMVADLTALPMANASLLDEATAAAEAMSLC
                     LAASRATAKTFFVADDCHPQTIAVVRTRAGAAGIEVRTGDARQIDFSAGELFAVLVQY
                     PTTDGRVRDYAPLAHAAHEAGALLVVATDLLALTLLRPPGEFGADVAIGSSQRFGVPM
                     GYGGPHAAFLATREEFRRLIPGRIVGVSRDDRGKTGYRLALQTREQHIRRDKATSNIC
                     TAQVLLAVMAAMYGVYHGADGLRRIARRVHDLTRMLANGLHRLGWSTGDEPFFDTLRV
                     EVGREDAQRVQAAAHRHRINLRDYGDGSLGVALDETATEEDLQGLLEAFALDRPLELS
                     FDELAHGDFDAIFARTSEFMTAECFKLYHTEHEMLRYLKRLQDRDLSLAHAMIPLGSC
                     TMKLNGTSQMMPVSWPEFAALHPFAAADQRLGYEKMFRQLESWLAEITGFRAVSLQPN
                     AGAQGEYAGLLVIRAYHRERGQAERDVCLIPTSAHGTNPASAVMAGLRVVPVVCDAAG
                     NIDLGDLERKADEHADRLAAIMITYPSTHGVFEHGVRRICDIVHRRGGQVYLDGANLN
                     AQVGLCRPGDYGADVCHLNLHKTFCIPHGGGGPGMGPIAVAEHLVDFLPGHPLVPTGG
                     DRAIGPVSAAPYGSAGILPISWMYIAMMGADGLRRASQVAILNANYMAHRLRDHFPIL
                     YSGAGGRVAHEFIIDLRPFKESAGVTVDDVAKRLMDYGLHAPTMSWPVAGTFMIEPTE
                     SESRDELDRFCEAMIAIRAEIREIEEGQADRADNLLKRAPHTAEALLDDGWDRPYGRE
                     RAAFPLPWVRERKFWPAVGRVDNAYGDRHLVCTCASVEAYVEP"
     gene            5290..>6699
                     /locus_tag="VD788_14280"
     CDS             5290..>6699
                     /locus_tag="VD788_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77478.1"
                     /translation="MIHARLAGVVVAAGLIGVAALAYTPTLEFGYAQDSWPAVKANPV
                     VERGDLREVFTSDYWKGTGSFARTLYRPVAVASFAIERRLSGEADPRLSHLINILLHG
                     LTAWLFYRLSRVLGAAEPVAIAAAVLFVLHPLALHTVANVVGRADTLAVLFSLVALRC
                     LASPRRRLAAWGMAAAVWLALGSKEIGAAVVPLLAVAEAIYRFPRGDARDAWRDRLAV
                     FGPTVAAVLLYLHLRTLAIGEFPGWQPLAIEDNLLVGLSGLQRVATTLAMAGRYALLS
                     VAPVGLSPDYSGGVIQAETSLLAPLPLLGVALSAAGIALVALGIPRGGGAPGPTRRLC
                     RFAAALVVVPYLFVGNVLTLNAAGFAERMVYFSSTGFCLLVAIAAGAAIARLAAGRVR
                     VTRAAWVAVVVVCASLELVQLRRHLPMWEDNRALFDYTARVAPRSLRAALQVAGRHEA
                     EGRPDEALEIYRRIETFAPD"
BASE COUNT          908 a         2312 c         2412 g         1067 t
ORIGIN      
        1 gggtgtgatc cagccgctcg aggaggtcgg acgcgcctgc cgcgaccgcg gaatcccgtt
       61 cgtcgtcgac gcggtgcagg cggcgggcaa gctgccggtc gacccgaagc cggtcttcgc
      121 cgacctgctg gccgtgtcga gtcacaagct gggcgggccg cagggggcgg gcgcgttgat
      181 cgtccgctcg ggtatcgagc tggcgcggct gatcggcggc ggcgcgcagg agaaacgccg
      241 gcgcggcggg accgagccgg tcgtcgcgat cgccggcttc ggcgccgcgg cgcgcgccgc
      301 gctcggcggg atgaagcgcg acaacgcgag gatgctgcac ctgcgagcga agatcgagac
      361 ccgtttgcgc gagctgttcc ccgatctgcg tttccacggc caggatgctc cgcggttgcc
      421 caacacggtc aacttcgcgc tgccggacgt accgggcgag atgctggtga tcgccgccga
      481 tctcgccggg atcgcgttgt cgaccggatc ggcgtgcgcg tcgggcgcgg tcgagccgtc
      541 ccacgtgatc ctggcgctcg gtcgcagcgc ttccgaggcg aggaacgcgg tgcggctatc
      601 ggtcggctgg agcacgacgc atgaggaggt cgacctgttt ctcgaacggt tccccggcgt
      661 cgtgcggcgg gttcgcgacg gcctgaaggg cggggcggac tagtccgcgg cgaagatgcc
      721 gcggacggcg gccagcacct cgcgcacctc gaacggtttc gagaggaagc cccgactgta
      781 cgcggcgaag tagccgaggt tgtcctcggt ttggctcatg cccgagatga agatcgtgtg
      841 cccggcgagc tcgtcgtcca gctggcggat gcggtggtgc agcgtcacgc catccatgtc
      901 gggtagcccg aaatcgacga tcgcggcgtc gtagatctcg gcgcggatgc gttcgagcgc
      961 ttgcgacccg gtcgaagccg tgtcgacctc gtagtcctga tcgaccagca cctcgcgcag
     1021 caattcgacg accggcgcat cgtcgtcgac caccagaatc cgcttgggct gggacatggg
     1081 gcggattcta gcctccgccg agcggaaaat acagtgtcgg tggaagttcc tgaggtccgc
     1141 cggtccggtg cggaggcccg tttgacggct cgcgccgggc ccgttaccgt gatgccccgg
     1201 acgatgacga tggcgcccag gaatcgaccg atgacggcgg tggcgatgag cggcggggtg
     1261 gacagctccg tcgcggcgtc gctgttggcg cgcgacgggc gaagcgtcgt cggcttctcg
     1321 atgcagctgg tcgaccggct ggccggtgag caggagcgct acggccgctg ctgttcgccc
     1381 gacgacttcc gcgacgcccg ccgcgtcgcc gatcgctacc ggttcccgca ctacatcctc
     1441 gacatggagc gcgagttcga gcggctcgtg ctgcgtccgt tcgccgacga ctacgccacc
     1501 gggcgcaccc cgtcgccctg cgtgcgttgc aacaccttca tcaagttcgg cgcgctgttg
     1561 tcgcgtgcgc gggccgtcgg cgccgaccgc gtggcgaccg gccactacgc gatcctcgag
     1621 cccgaccgcc gcaccggccg cacgttgctc cggcgcgcgg tcgaccgcga caaggaccag
     1681 agctacttcc tgttcgatct gtccgacgag caacgccagg cggccgagtt cccgctcggc
     1741 cgcttgacca aggaccgcgt gcgacagctc gcggcggaaa tgggcttggc cgcggcggac
     1801 aagcccgagt cgatggatct gtgcttcgtc gcgcgggacg agagctaccg cgacttcctc
     1861 gcgcgcaacc gactcgcgcc ggcggctgcg ccgggcgagg tcgtcgacgg caccgggcgc
     1921 gtgctcgggt ggcacgacgg ggtctcccgt ttcaccgtcg gccagcgccg cggcctcggt
     1981 atttcggctg cggagcggtt gtacgtcatc ggggtcgacc cctcgacgcg aagggtcgag
     2041 gtcggagggg atgccgcgct ctatcgcgat cgctgcgtga tcgagcgcac gcgctggatc
     2101 gcgttcgagc gaccgggcgg agcgctgcgg gcgtcggtcc ggatccgctc gacgcacgag
     2161 ggcgccgcgg cgtcactccg cgagctgggc gacgggcgcg cggagatccg cttcgaccag
     2221 ccgcagcgcg cgatcaccgt gggacaggcc gccgtggcct acgacggcga cctcgtgctg
     2281 ggtggtggct ggatcgccgc cgcctgatac cctggaccgg gtcggggccg ggtggggttg
     2341 tcgcgcttcg accgatctgc tagaagcaat ccccggagca tctctggcat aggtgccgtc
     2401 acgatgaaga accgctcgcc cgtcgatccc accgatctgt tcgccccgcg tcacatcggc
     2461 tccgcgcccg accaactgca ggcgatgctc ggctcgctcg gattcgactc gctcgaccgt
     2521 ctgtccgacg cgatcgttcc cgacgacatc cgcttgcggc gcgacttgcg cctgggcccc
     2581 ccgcgcagcg agagccgagc gctggccgag ctgcggcaga tcgcgcgcca caacgaggtc
     2641 tatcgttctt tcatcggcct gggctatcac ggctgcatca cgccgccggt gatccagcgc
     2701 aacatcttgg agaacccggg ctggtacacg caatacacgc cgtaccaggc cgagatcgcc
     2761 cagggtcggc tcgaagcgct gttgaacttc caaacgatgg tcgccgacct gaccgcgctg
     2821 ccgatggcca acgcgtcgtt gctcgacgag gcgacggcgg cggcggaggc gatgtcgttg
     2881 tgcctggccg ccagccgcgc cacggccaag acgttcttcg tcgccgacga ctgtcacccg
     2941 cagacgatcg ccgtcgtgcg aacccgagcg ggcgcggcgg ggatcgaggt gcgtaccggc
     3001 gacgcccggc agatcgactt ctcggccggc gagctgttcg ccgtgctggt gcaatatccg
     3061 acgaccgacg ggcgcgtgcg cgactacgcg ccgctggccc acgccgcgca cgaggcgggc
     3121 gcgctgctcg tcgtcgccac cgacctgctg gcgctgacgc tgttgcgtcc gccgggcgag
     3181 ttcggcgccg acgtcgcgat cggttcgagc caacggttcg gcgtgccgat gggttacggt
     3241 ggcccgcacg cggcgttcct cgcaacgcgc gaggagttcc ggcggctgat acccggccgg
     3301 atcgtcggcg tgtcgcggga cgatcggggc aagaccggct accggctggc gctccagacc
     3361 cgcgaacagc acatccgacg cgacaaggcg acgagcaaca tctgtaccgc gcaggtgctg
     3421 ctggccgtga tggccgcgat gtacggggtc taccacggtg ccgacgggtt gcgccggatc
     3481 gcgcgacgag tccacgatct gacgcggatg ttggccaacg gcctgcaccg cctcggttgg
     3541 agcacgggcg acgagccgtt cttcgatacg ttgcgcgtcg aggtcggacg cgaagacgcc
     3601 cagcgcgtgc aggccgccgc gcaccggcat cgcatcaatc tgcgcgacta cggcgacggc
     3661 tcgctgggcg tcgcgctcga cgagacggcg accgaggagg acctgcaggg cctgctcgag
     3721 gcgttcgcgc tggatcgccc gctcgagctg tcgttcgacg agctggcgca cggcgacttc
     3781 gacgcgatct tcgcgcgcac ctccgagttc atgaccgccg agtgcttcaa gctgtaccac
     3841 accgagcacg agatgctgcg ctacctgaag cgactgcagg accgcgatct gtcgctggcc
     3901 cacgcgatga tcccgctcgg ctcgtgcacg atgaagctca acgggacctc gcagatgatg
     3961 ccggtgagct ggccggaatt cgccgcgctg cacccgttcg ccgccgccga ccagcggttg
     4021 ggctacgaga agatgttccg gcagctcgag agctggctgg ccgagatcac cggcttccgc
     4081 gccgtgtcgc tgcagcccaa cgccggcgcg cagggcgagt acgccggcct gctcgtgatc
     4141 cgcgcctatc accgcgaacg cggacaagcc gaacgcgacg tctgcctgat cccgacctcg
     4201 gcccacggca ccaacccggc gagcgcggtg atggcggggc tgcgcgtcgt gcccgtcgtc
     4261 tgcgacgcgg ccggcaacat cgacctcggt gatctcgagc gcaaggccga cgagcacgcc
     4321 gatcggctgg ccgcgatcat gatcacctat ccctcgacgc acggcgtctt cgagcacggg
     4381 gtgcgacgga tctgcgacat cgtgcatcgg cggggcggtc aggtctatct cgacggtgcc
     4441 aacctcaacg cgcaggtcgg actgtgccgt cccggcgact acggcgccga cgtctgtcat
     4501 ctcaacctgc acaagacctt ctgcatcccg cacggcggtg gtgggccggg catgggaccg
     4561 atcgcggtcg ccgagcacct ggtcgatttc ctccccggtc acccgctggt gccgaccggc
     4621 ggagaccgcg cgatcggccc ggtgtcggcg gcgccgtacg gcagcgccgg gatcttgccg
     4681 atcagctgga tgtacatcgc gatgatgggc gccgacggat tgcgccgggc gagccaggtc
     4741 gcgatcctca atgccaacta catggcccac cgcctgcggg accactttcc gatcctctac
     4801 agcggcgccg gcggccgcgt ggcccacgag ttcatcatcg atctgcggcc gttcaaggag
     4861 agcgccgggg tgaccgtcga cgacgtggcc aagcggctga tggattacgg gctgcacgcg
     4921 ccgacgatgt cctggccggt ggcggggacg ttcatgatcg agccgaccga gagcgagtcg
     4981 cgcgacgagc tcgatcggtt ctgcgaggcg atgatcgcga tccgggcgga gatccgcgag
     5041 atcgaggagg gccaagcgga ccgcgccgac aacctgctca agcgcgcgcc gcacaccgcg
     5101 gaggcgttgc tcgacgacgg ttgggaccgg ccgtacggcc gcgagcgcgc cgcgttcccg
     5161 ttgccgtggg tgcgtgagcg caagttttgg ccagcggtcg ggcgcgtcga caacgcctac
     5221 ggcgatcggc acctggtctg cacttgcgcc tcggtcgaag cctacgtcga gccctgatcg
     5281 tccgacgccg tgatccacgc gcgcctggcc ggagtcgtcg ttgcggcggg actgatcggg
     5341 gtcgcggcgc tggcctacac gccgacgctc gaattcggtt acgcccagga ctcctggccc
     5401 gcggtcaagg ccaatccagt cgtcgagcgc ggcgacctgc gcgaagtctt cacctcggat
     5461 tattggaagg ggaccgggtc cttcgcgcgc acgttgtatc ggccggtggc cgtcgcgagt
     5521 ttcgcgatcg agcggcgcct gtcgggcgag gccgacccgc ggctgtcgca cctgatcaac
     5581 atcctgctgc acggactgac ggcctggctg ttctatcggc tgtctcgcgt gctgggcgcg
     5641 gccgagccgg tcgcgatcgc ggccgcggtg ctgttcgtgc tgcacccgct ggcgctgcac
     5701 acggtggcca acgtcgtcgg gcgcgccgac accctggcgg tgctgttctc actcgttgcc
     5761 ctccgctgct tggcttcgcc gcggcgccgg ctggcggcgt ggggcatggc ggccgccgtc
     5821 tggctcgcgc tgggctcgaa ggagatcggc gcggcggtcg tgccgctgtt ggccgtggcc
     5881 gaggcgatct accgcttccc gcggggcgac gcgcgcgacg cgtggcgcga tcggttggcg
     5941 gtcttcggcc cgaccgtcgc ggccgtcttg ctctatctgc acctgcggac gctcgccatc
     6001 ggcgagttcc ccggctggca gccgctggcg atcgaggaca acctgctcgt cggtctgagc
     6061 ggactgcagc gcgtggcgac gacgttggcg atggcggggc gctacgcgct gctgtcggtc
     6121 gcgccggtcg gtctgtcgcc ggactattca gggggcgtca tccaggccga gacgtcgctg
     6181 ctcgcgccgc ttccgctgct cggtgttgcg ctgtcggcgg cggggatcgc gctggtcgcg
     6241 ctcggcatcc cgcgcggcgg cggggcgccg ggcccgaccc gccgcctgtg ccggttcgcc
     6301 gcggcgctcg tcgtcgtacc gtacctgttc gtcggcaacg tgctgacact caacgcggca
     6361 ggcttcgccg agcgcatggt ctacttctcg tcgaccggtt tctgcctgct cgtcgcgatc
     6421 gccgccggtg ccgcgatcgc gcgtctggcc gccggccgcg tccgtgtgac ccgcgccgcc
     6481 tgggtcgccg tcgtcgtcgt ctgcgcctcg ctcgagctgg tccagctgcg acgccacctg
     6541 ccgatgtggg aggacaaccg tgcgttgttc gactacaccg cgcgggtggc accgcgtagc
     6601 ctgcgcgccg cgctgcaggt cgcggggcgg cacgaggccg agggccggcc cgacgaggcg
     6661 ctcgagatct accggcggat cgagaccttc gccccggac
//
LOCUS       DASPGN010000491         7972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1377215, whole genome shotgun sequence.
ACCESSION   DASPGN010000491 DASPGN010000000
VERSION     DASPGN010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1377215"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VD788_14285"
     CDS             complement(<1..780)
                     /locus_tag="VD788_14285"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HXV77479.1"
                     /translation="MTPTVVILLVGSSVAASCALVGTFLVLRRLALLGDAISHSVLPG
                     IVVAFLLTGNRAPIPMLAGAAALGVVTVVLVELFHRTRRLKEDASIGVVFPALFSIGV
                     ILIARYTSSIDLDLDCVLYGEIAYTPWDLAHIGGRSVGPRALWVNGSVLAIDGLLIAS
                     LYKELKLTTFDPKLAASIGFSPVLVHYALMSAVSVTVVGAFESVGAILVVAMLVVPPA
                     AAYLLTDHLGRMIALSVGIGVGSALAGYQLARWLDASIAGAM"
     gene            complement(777..2057)
                     /locus_tag="VD788_14290"
     CDS             complement(777..2057)
                     /locus_tag="VD788_14290"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HXV77480.1"
                     /translation="MTELSYTVRIVLAGATLLGTTGGVLGCFALLRRQSLLGDALAHA
                     ALPGVCTAYLLTYSKQPLPLFAGALVAGLLGAWTIVVITRRSRIKEDTAIGIVLSVFF
                     GIGILLLTYIQRLPTGNQSGLDRLLFGQAATLLERDVLQMTLLAGAVLLVVGLLFKEF
                     KLLCFDREFAASSGLPTRRLELLLTTLLVVVVVVGLQTVGVVLIVATLITPAAAARQW
                     TERLGVMLLLAGAIGGTAGAGGAMLSATVADLPTGPVIVLCASSVLVVSLLLAPRRGL
                     VWATIDEHRVARRIRRENLLKDLYTWIERHGGAWSQRVPASFVMGLRGDSGRRIGRTA
                     RRLAASGSIDYAPGWLSLTDRGLRDAERVVRKHRLWELYLTRRLELASDHVHRDADTM
                     EHALTDEAVAKLEQVLGHPTRDPHGRPIPPGRAA"
     gene            complement(2054..2872)
                     /locus_tag="VD788_14295"
     CDS             complement(2054..2872)
                     /locus_tag="VD788_14295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HXV77481.1"
                     /translation="MDLAQSPAASPAAIEVHDLTVAYRTQPVLWDIDLRLPEGKLTAI
                     VGPNGAGKSTLLKAMLGLVQPITGWVKVFDAPYARRRSWVGYVPQRESVDWDFPTSAL
                     DVVTMGLYGRLGWFRRPGRREREAALACLDHVGMRELASRQISQLSGGQQQRVFLARA
                     LAQQARLYLMDEPFAGVDATTERAILTLLEELKQSGRTVVAVHHDLQTVAEYFDHVVL
                     LNMRLVAAGPVAEVFTPENLQRTYGGRLTVLTQATEALLRAPAADPALQRGLRE"
     gene            complement(2894..3817)
                     /locus_tag="VD788_14300"
     CDS             complement(2894..3817)
                     /locus_tag="VD788_14300"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HXV77482.1"
                     /translation="MSKTPFASLAGLLVLHAIACGGGDAASPDRRLLVVTTTGIVGDM
                     ARRIAGGDAEVVSLMGPGVDPHLYKASESDVRRLAEADLILYNGLHLEGKMGDILVKM
                     ARSRPVVAVTETIPDDRLREPPEFAGQYDPHVWFDVDLWSSTIAPIVDALGELAPERA
                     TSFRDNGDRLRDELRELDSWIERRIGELPEERRILVTAHDAFGYFGRRYGMRVVGLQG
                     ISTLDEAGLKDVDRVVDLVVGERVSAIFVESSVPRRSIEAVQAAAAERGHRVAIGAEL
                     YSDALGAGGTPAGTYIGMVRHNVDSIVQALQ"
     gene            complement(3873..5204)
                     /locus_tag="VD788_14305"
     CDS             complement(3873..5204)
                     /locus_tag="VD788_14305"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77483.1"
                     /translation="MSKRIGEILVQRGLLDQEQLEQALNAQLVYGGHLGTCLIERGFV
                     NEDELGQALAETYGVGYASAKELTDVPGPIIECLPRKIVEKHSMIPFRVRNGTLDLAL
                     INPKNVEALTEAACTTGFRARAWVAPEIRIYQALERYYGVPRRLRFVALCRELERDPP
                     AEPPARRDRPPRAARPPAAAPDPPPAAREPVYDLGLTPADTGAGVSRDLEDLGAEFGY
                     GQSWQEIAQDSLWTERSSPGRSPSPRSPSVVPAAPRPGLVPTPARASVAPAPPRPVAS
                     TPRPAVLPLDAASQRLADARTTDDVASSFLDWAVAGTPRCLLWRVDGLTAILWDWRGF
                     AIDAARRSRLTLKVTEEPVFRLVAGSTCYRGPVPPAPEYRAFFERLGVDEPSEVEVVP
                     LHMDDRLVALFYGDSGTRPTVSTPSLDYRRALLKLGYALWMLRLRQKILGA"
     gene            complement(5280..6488)
                     /locus_tag="VD788_14310"
     CDS             complement(5280..6488)
                     /locus_tag="VD788_14310"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HXV77484.1"
                     /translation="MSRRVVVTMPGDGIGRVVLPHAVAVLEAAGFDADYVEAPIGWDC
                     WLEQGNALPQRTVELLERHRLGLFGAITSKAKTQAEAELRPELRRHGLRYTSPIVALR
                     QRFDLDVCIRPCRSFPGNPLNFVRRTASGAIEEPSIDAVVFRQNTEGLYVGLEWTDPP
                     AAVLDALGSHPRFRPFAGVPPADIAITTRLFTRNACRRIVHAAYDYALRHGRASVTVC
                     EKPNVLQETSGMLEQVAREVQSSFPAIEHRAVNIDTQMLVLTKMPETLGVIVAGNEFG
                     DILSDGIAGLVGGLGFVASANLGERIAVFEPIHGSAPKYASVDPPIANPIAMILAAAM
                     LAEYAGDAPRAATIRQAVARVVRAGRVRTYDMLRVPGGADVRSSGAATTGEMAQAVIA
                     ELEPLRQVAD"
     gene            complement(6485..>7972)
                     /locus_tag="VD788_14315"
     CDS             complement(6485..>7972)
                     /locus_tag="VD788_14315"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HXV77485.1"
                     /translation="AIWATGEFWWRVPPTVRVVLRGGLRGGAVGKDVIIALCSFFRRG
                     EVRNAAVEFGGPGVDGLTIDDRLAIANMTTEWGALVGWFPPDGAVEAFLAGLEARRTE
                     PVGPPLEPDPAARYAGTISLDLDSVCPHVAGPDQVSIARPLGEVQSQRIRVDKAYLVS
                     CANSRASDLAAAAEALGGRSVADGVTLYVSAASALVQRQAERDGSWARLLEAGAKPLP
                     PGCGPCIGLGAGLLAPGEVGVSATNRNFKGRMGSPDARCYLASPAVVAASAAAGYIRG
                     PAARPRARLRAAFALSPDEPDPAAGSELLDGFPASLRGRLLFIALDDVVTDRIFPGRL
                     TYREDLSRAAIAAAAFADLDPSLAPRMLPGDLLVTGRGFGGGSSREQAVTALQAAGVA
                     AVVAASFSSTYLRNALNNGFLCIESPATFEYLRARFGANDPRPVRVTDEQIEVDFASR
                     AIVVHGRSFAFLPPGPVGQRLLLDGGIERSVRRRVDALRASGGGS"
BASE COUNT         1320 a         2828 c         2743 g         1081 t
ORIGIN      
        1 catcgcgccg gcgatcgatg cgtcgagcca gcgcgcgagc tggtagccgg cgagcgcgga
       61 gccgacgccg atcccgaccg acagcgcgat catccggccc agatgatcgg tcagcaggta
      121 cgcggcggcg ggcggtacga ccagcatcgc gacgacgagg atcgcaccga ccgactcgaa
      181 ggcgccgacg acggtcaccg acacggcgct catcagcgcg taatggacca gcaccggcga
      241 gaagccgatc gaagccgcga gcttcgggtc gaacgtcgtc agcttgagtt ccttgtagag
      301 cgaggcgatc aacagcccgt cgatcgccag caccgatccg ttgacccaca acgctcgcgg
      361 tccgaccgac cgcccaccga tatgggccag gtcccacggg gtgtaggcga tctcgccgta
      421 cagcacgcag tccaagtcga ggtcgatgct cgacgtgtag cgcgcgatca ggatcacacc
      481 gatcgagaac aacgccggga agacgacgcc gatcgacgcg tcctccttca gccgccgggt
      541 gcgatggaac agctcgacca gcacgaccgt caccacgccg agcgccgctg ccccggcaag
      601 catcggaatc ggcgcgcggt tgcccgtcag caggaaggcg acgacgatcc cggggaggac
      661 cgaatggctg atcgcgtcgc cgagcaacgc caagcgccgc agcacgagga aggtcccgac
      721 gagcgcacag gaagcggcca cgctgctccc gaccagcagg atcacgacgg tcggagtcac
      781 gccgcccgcc ccggcgggat cggtcgcccg tgcggatccc gggtcgggtg gcccagcacc
      841 tgctcgagct tcgccaccgc ttcgtcggtc agggcgtgtt ccatcgtgtc ggcgtcgcga
      901 tgcacgtggt ccgaggccag ctcgaggcga cgggtgaggt agagctccca caggcggtgc
      961 ttgcgcacca cgcgctcggc gtcgcgcagg ccgcgatcgg tcagtgacag ccagcccggg
     1021 gcgtaatcga tcgaaccgct cgccgcgagc cgccgcgcgg tccgaccgat gcgacggccg
     1081 ctgtcgccgc gcagccccat cacgaacgaa gccggcacgc gctgcgacca cgcaccgccg
     1141 tgacgctcga tccaggtgta gaggtctttc agcaggttct cccggcggat gcgtcgcgcg
     1201 acgcggtgct cgtcgatcgt cgcccaaacc aacccgcgtc ggggcgcgag cagcagcgaa
     1261 acgacgagca ccgagctggc gcagagtacg atgaccggac cggtcggcag gtcggcgacc
     1321 gtcgcgctga gcatcgcccc gccggcgccg gcggtgccgc cgatcgctcc ggccagcagc
     1381 agcatcacgc ccagccgctc ggtccactgc cgggcggcgg ccgcgggcgt gatcaacgtc
     1441 gcgacgatca gcaccacgcc gaccgtctgc aggccgacga cgacgaccac gaccagcaat
     1501 gtcgtcagca gcagctcgag gcgccgggtc ggcagaccgc tcgaggcggc gaactcccgg
     1561 tcgaagcaca gcagcttgaa ctccttgaac agcagcccca cgaccagcag caccgccccg
     1621 gcgagcagcg tcatctgcag cacgtcgcgt tcgagcaagg tcgcggcctg gccgaacagc
     1681 aggcgatcga gcccgctctg attgccggtg ggcaggcgtt ggatgtacgt cagcagcagg
     1741 atgccgatac cgaagaagac ggacaagacg atgccgatcg cggtgtcttc cttgatgcgg
     1801 ctgcggcgtg tgatcacgac gatggtccac gcgcccagca gacccgcgac gagagcgccg
     1861 gcgaacagcg gcaacggctg cttgctgtag gtcagcaggt aggcggtgca gacgcccggg
     1921 agagccgcgt gggccaaggc gtcccccagc aagctctgcc ggcgcagcag cgcgaagcag
     1981 ccgagcacgc cgccggtcgt accgagcagc gtcgcgccgg cgagcacgat gcgaacggtg
     2041 taggagagtt cggtcactcg cgaagacccc gttgcagcgc ggggtcggcc gccggcgcgc
     2101 gcagcagcgc ctcggtggcc tgggtcagta ccgtcagacg gccgccgtag gtccgctgca
     2161 ggttctcggg cgtgaacacc tcggccaccg gtcccgccgc caccagacgc atgttgagca
     2221 acacgacgtg gtcgaagtac tcggcgaccg tctgcaggtc gtggtgcacc gcgacgaccg
     2281 tccggccgct ctgcttcaac tcctcgagca aggtcaggat cgcccgctcg gtcgtcgcgt
     2341 cgacgccggc gaacggttcg tccatcaggt acagcctggc ctgttgcgcc agcgcccgcg
     2401 cgagaaacac ccgctgctgc tggccgccgg aaagctggct gatctgccgc gacgcgagct
     2461 cgcgcatgcc gacgtggtcc aggcaggcca gcgcggcctc gcgctcgcgc cgccccggcc
     2521 ggcgaaacca acccagccga ccgtacagac ccatcgtcac cacgtcgagc gcgctggtcg
     2581 ggaagtccca gtcgacgctc tcacgctgcg ggacgtagcc gacccacgat cggcgccggg
     2641 cgtacggcgc gtcgaagacc ttgacccacc ccgtgatcgg ctgcaccagc ccgagcatcg
     2701 ccttgagcag cgtgctcttg cccgcgccgt tcggaccgac gatcgccgtc aacttccctt
     2761 cgggaagccg caggtcgata tcccacagga ccggctgggt ccggtaggcc accgtcagat
     2821 cgtgtacctc gattgcggcc ggggaagcgg ccgggctttg cgctaggtcc atcaccagca
     2881 cctctcgagt cggtcactgc agtgcttgca cgatactgtc gacgttgtga cgcaccatgc
     2941 cgatgtaggt tcccgcgggg gtgcccccgg cacccaacgc gtccgagtac agctccgccc
     3001 cgatcgcgac ccggtgaccg cgttcggcag cggccgcttg cacggcctcg atcgaacggc
     3061 gcggcacgct cgactcgacg aagatcgcgc tcacgcgctc gccgacgacg agatcgacca
     3121 cgcgatcgac gtccttgagc ccggcttcgt cgagcgtgct gatgccctgc aggccgacga
     3181 cccgcatccc gtaccggcga ccgaagtagc cgaaggcgtc gtgcgcggtg acgagaatcc
     3241 gtcgttcctc gggcaattcg ccgatgcggc gttcgatcca cgagtcgagc tcccgcaact
     3301 cgtcgcgcag gcggtcgccg ttgtcgcgga agctcgtggc tcgctccggc gccagttcac
     3361 cgagcgcgtc gacgatcgga gcgatcgtcg aggaccagag gtcgacgtcg aaccagacgt
     3421 gggggtcgta ctgaccggcg aactcgggtg gctcgcgcaa gcgatcgtcg ggaatcgtct
     3481 cggtcaccgc cacgaccggc cggctcctcg ccatcttgac gaggatgtcg cccatcttcc
     3541 cctccaagtg cagaccgttg tagaggatca ggtcggcctc ggcgagccgg cgcacgtcac
     3601 tttcgctcgc tttgtagagg tgcgggtcga ccccgggtcc catcagcgag acgacctccg
     3661 cgtcgccccc ggcgatccgg cgggccatgt cgccgacgat cccggtggtg gtgacgacca
     3721 ggagtcgccg atcgggcgaa gccgcgtcgc cgccgccgca ggcgatcgcg tggagcacga
     3781 gaagaccggc cagcgatgcg aagggggttt ttgacatatc aaaaaaatag acacagaccg
     3841 cccaaaaaag caagaccggg ctgcgggccg acctacgcgc cgaggatttt ctggcgcaaa
     3901 cgcagcatcc acagcgcgta gcccagcttg agcaacgctc ggcggtagtc caagctgggg
     3961 gtcgagaccg tcgggcgcgt gccgctgtcc ccgtagaaca acgcgacgag gcggtcgtcc
     4021 atgtgcaacg ggaccacctc gacctcgctc ggttcgtcca cgccgagccg ttcgaagaac
     4081 gcccgatact ccggtgcggg cggaaccgga ccgcggtagc aggtcgaccc ggcgaccagc
     4141 cgaaagaccg gctcctcggt cactttgaga gtcagtcgcg acctccgtgc ggcgtcgatc
     4201 gcgaagccgc gccagtccca caggatcgcg gtcagtccat cgacccgcca cagtaagcag
     4261 cgcggcgtgc cggccaccgc ccaatcgagg aagctcgatg cgacgtcgtc ggtcgtgcgc
     4321 gcgtcggcga gccgttgcga ggcggcgtca agcgggagca cggcgggtct cggcgtgctg
     4381 gcgacaggcc ggggcggggc cggagcaacg gacgcgcgtg ccggagtggg cacgagcccg
     4441 ggccggggcg ccgccggcac gacgctcggc gaccggggag acggcgacct ccccggcgag
     4501 gaccgctcgg tccagaggct atcttgggcg atctcctgcc acgactgccc gtaaccgaac
     4561 tccgccccca ggtcctcgag gtcgcggctg acgccggcac cggtgtcggc tggggtcaag
     4621 cccaggtcgt agaccggctc ccgggccgcg ggcggcggat cgggcgctgc cgcgggcggt
     4681 cgcgccgcgc gtggtggtcg gtcgcggcgg gccgggggct ctgccggcgg gtcacgctcg
     4741 agctctcggc acagggcgac gaagcgcagg cgccgcggca cgccgtagta ccgctcgagc
     4801 gcctggtaaa tccggatctc gggcgcgacc caggcccgcg ctcggaaacc ggtcgtgcac
     4861 gcggcctcgg tcagcgcctc gacgttcttc gggttgatca acgccaggtc gagcgttccg
     4921 ttccgcacgc ggaacgggat catcgagtgc ttctcgacga tcttccgcgg aaggcactcg
     4981 atgatcggcc ccggaacgtc ggtcagttcc ttggccgacg cgtagcccac gccgtaggtc
     5041 tcggcgagcg cctgccccag ctcgtcttcg ttgacgaacc cccgctcgat cagacaggtt
     5101 ccgaggtgcc cgccgtagac caactgggcg ttgagtgctt gttcgagctg ttcctggtcg
     5161 agcaagcctc gctggacgag gatctctccg attcgcttcg acattcccga ccccggaccc
     5221 cgggcgaggg gccaacctac cgagctcaac gaggaatctg ggttccgcgg cgggcagttt
     5281 caatccgcga cctgtcggag gggttcgagc tcggcgatca cggcctgcgc catctcgccc
     5341 gttgtcgccg cccccgacga tcggacgtcg gctccgccgg gcacgcgtag catgtcgtag
     5401 gtccgcaccc gcccggcacg cacgacccgc gcgacggcct gacggatcgt cgccgcgcgc
     5461 ggggcatcgc cggcgtactc ggcgagcatc gccgccgcca ggatcatcgc gatcgggttg
     5521 gcgatcggcg ggtcgaccga tgcgtacttc ggcgccgagc cgtggatcgg ctcgaacacc
     5581 gcgatccgtt caccgagatt ggccgaggcg acgaatccca gcccgccgac gaggcccgcg
     5641 atgccgtccg acaggatatc gccgaactcg ttgccggcga cgatgacccc gagcgtttcg
     5701 ggcatcttcg tcaagacgag catctgcgta tcgatgttga ccgcgcgatg ctcgatcgcc
     5761 gggaacgaag actgcacctc gcgggcgacc tgctcgagca tgccggaggt ctcctgcagc
     5821 acgttcggtt tctcgcagac ggtgacggag gcgcgaccgt ggcgtaacgc gtagtcgtag
     5881 gccgcgtgca cgatccggcg gcaggcgttc cgcgtgaaca gccgtgtcgt gatggcgata
     5941 tcggcaggcg gaacgccggc gaacggccgg aaccgcggat gggagccgag cgcgtcgagc
     6001 acggccgccg gcgggtcggt ccactcgagg ccgacgtaga gtccttcggt gttctggcgg
     6061 aacacgaccg cgtcgatcga cggttcctcg atcgcgcccg atgcagtgcg ccggacgaaa
     6121 ttgagcgggt tgcccgggaa gctgcgacac ggacggatac acacgtccag atcgaagcgc
     6181 tggcgcagtg cgacgatcgg gctcgtgtaa cgcagaccgt ggcggcgcag ctccgggcgg
     6241 agctccgcct cggcctgggt cttggccttc gaggtgatcg cgccgaacag accgagccga
     6301 tggcgctcga gcagctcgac cgtgcgctgc ggcagggcgt tgccctgctc gagccagcag
     6361 tcccagccga tcggggcctc gacatagtcg gcgtcgaagc cggcggcctc cagcacggcg
     6421 acggcgtgcg gcagcacgac cctgccgatg ccgtcgcccg gcatggtaac gaccacgcgg
     6481 cggctcacga cccgcctccg ctggcccgga gcgcatccac gcgccggcgc acgctgcgct
     6541 cgattccacc atcgagcagc aggcgctggc cgaccggccc cggtggcagg aacgcgaagc
     6601 tccggccgtg gacgacgatc gctcggctgg cgaagtcgac ctcgatctgc tcgtcggtca
     6661 cgcggaccgg ccgcgggtcg ttcgctccga atcgagctcg taggtactcg aacgtcgccg
     6721 gcgactcgat gcagaggaag ccgttgttca gcgcgtttcg cagatacgtc gacgagaacg
     6781 aagccgcgac aacggctgcc acgcccgccg cctggagcgc ggtcaccgcc tgctcgcggc
     6841 tcgacccgcc accgaagcct cggccggtga ccaacaggtc ccccggcaac atgcgcggcg
     6901 ccaagctcgg atcgagatcg gcgaacgcgg ccgcggcgat cgccgcacga gaaaggtctt
     6961 cgcggtaggt caatcggccg gggaagatgc ggtcggtgac gacgtcgtcg agcgcgatga
     7021 acaacagccg gccacgcaag ctcgcgggga agccgtcgag caactcgcta ccggccgctg
     7081 ggtcgggctc atccggcgac agtgcgaacg ccgcccgcaa tcgcgctcgc ggtcgggcgg
     7141 ccggaccgcg gatgtagccc gcggccgcgg atgccgccac gacggccggg ctcgccagat
     7201 aacagcgggc atcgggcgac cccatccgtc ccttgaagtt gcgattggtc gcggagacgc
     7261 cgacctcgcc cggtgcgagc agccccgctc cgaggccgat gcacggtccg cagcccggcg
     7321 gcaacggctt cgcaccggcc tcgagcaatc gcgcccacga accgtcgcgc tcggcctgcc
     7381 gttgaacgag ggcactggcg gccgacacgt agagcgtcac accgtccgcc accgaccggc
     7441 cgccgagcgc ctcggcggcg gccgcgagat cgctcgctcg ggaattggcg cacgagacga
     7501 gataggcctt gtcgacccgg atacgctgcg actgcacttc gccgagcggc cgcgcgatcg
     7561 acacctgatc cggtccggcg acgtgcgggc atacgctgtc gaggtcgagg ctgatcgtac
     7621 cggcgtagcg tgccgccgga tccggctcga gtggcggtcc caccggttcc gtccgccgcg
     7681 cctcgagtcc ggcgaggaac gcctcgacgg caccgtcggg cggaaaccaa ccgaccagcg
     7741 ctccccattc ggtggtcatg ttggcgatcg ccaggcgatc gtcgatcgtc agtccgtcga
     7801 cgcccggccc gccgaactcg acggcggcgt tgcgcacttc gccgcggcga aagaacgagc
     7861 acagcgcgat gatcacgtcc ttgccgaccg cgcctccgcg cagcccgcct cgcaacacca
     7921 cgcggaccgt cggcggcaca cgccaccaga actcgccggt ggcccagatc gc
//
LOCUS       DASPGN010000492         3970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_676459, whole genome shotgun sequence.
ACCESSION   DASPGN010000492 DASPGN010000000
VERSION     DASPGN010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676459"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1518)
                     /locus_tag="VD788_14320"
     CDS             complement(34..1518)
                     /locus_tag="VD788_14320"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HXV77486.1"
                     /translation="MADELVVALRWGTLGLYFACLGVLTLYGIHRWYLVALYLRHRDR
                     AVVPPGRLEPLPPLTVQLPLFNEMYVVERLIDAVCALDYPRDRLEIQVLDDSTDETTA
                     LVRRKVEQKVGEGFDIVHLCRTDRGGFKAGALETGQRRAKGELIAVFDADFVPRPDFA
                     RQLVHHFVDPRVGMVQARWGHLNADHSPLTRVQSMLLDGHFVIEHTARNRSGRFFNFN
                     GTAGIWRRECIVDAGGWQHDTLTEDLDLSYRAQLGGWRFVFVNEHVAPAELPIEIGSF
                     KSQQHRWAKGSIQTARKLLPAILTGRLPFRVKLESAVHLTANVGYVLMVLLALLVVPA
                     VYLRAEISPWWIVAVDLPLFTFSSLSVAAFYVLAHGEATGRRGGIARWVPFLMAVGIG
                     LSINNARAVIEALAGRDSEFHRTPKYNLRRGENIAARSYRVTINRDTWVELALALYFS
                     VATAAVIASGLWGAVPFLLLFVVGYSFTALLALRQAARRAAWPV"
     gene            1625..2959
                     /locus_tag="VD788_14325"
     CDS             1625..2959
                     /locus_tag="VD788_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77487.1"
                     /translation="MIPVLAVLSAAIYGALAFAGDLTQRPTVLYGGCGLLLAAMWAAW
                     CLSRGGATRLRPILAAALLFRLIAACGPPALSDDVYRYVWDGRVQLHGIHPYLFAPTD
                     PRVEALRDENWRRINHPQLITIYPPVSQLLFAALAASGAGPGGFRIALGLVDFGVVLA
                     LSLLLRRLGLPGERVVLYAWNPLAVLEASGSGHVEPLGIAFVLLATAWIIGCRHRLST
                     AALAAAIHVKLLPVVLIPVFAPSYRIRGVVWLVLWLLVLWAPYGLSGPAFGAGLWAYA
                     ESWEHNSSIFALVETALVALDSGRLLEPLVSSVKERSGAAWVPWDSLYRWLWPPGLAR
                     ALVAGAGIVWVVAVARRDGIPLARRCLLAIGGVLLLSPTLHPWYVLWVLPFAAALLSW
                     PWLVLAALVPLSYLGAGADPPPWVLLVEYGMPCGIVVVDRLARRSTSYAVDR"
     gene            2956..3948
                     /locus_tag="VD788_14330"
     CDS             2956..3948
                     /locus_tag="VD788_14330"
                     /inference="COORDINATES: protein motif:HMM:NF013665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HXV77488.1"
                     /translation="MNVLDAILSRARRDPVRIAMPEGEDPRVLRAALRLARERIATPL
                     LVGEAQTVRLAAERASVELGPVAIVEPGRHAAHDRCAHAAREALAPRGATAEEVERCL
                     GDPLYYAASMVRAGLADGTLAGATHSTAETLRAALRIIGPARSTGTVSSFFLMALRQP
                     TSAGDDVLAFADCGLIPYPDAAQLATIARQTAAQFRLLVEREPRVALLSFSTAGSASH
                     PSVDSVRAARDLLAAGDADFEFDGEMQVDAALVPDVARAKLARSPVAGRANVLIFPNL
                     DAGNIAYKLVERLAGATALGPLVQGLARPFMDLSRGCKPDDIVSVACIASALAD"
BASE COUNT          531 a         1437 c         1374 g          628 t
ORIGIN      
        1 gttccgtggt ccgacgccga cctgcggtag ccgtcaaacc ggccaggcgg cccgccgcgc
       61 cgcctgacgc agcgcgagca gcgccgtgaa cgaatagccg acgacgaaca gcagcaggaa
      121 cggcacggcg ccccacagcc cgctggcaat caccgcggcc gtggccacgc tgaagtagag
      181 cgcgagcgcc agctcgaccc acgtgtcgcg attgatcgtc acccggtagc tgcgggcggc
      241 gatgttctcg cctcgccgca ggttgtactt cggcgtgcgg tggaactccg agtcgcgtcc
      301 ggccagcgcc tcgatcacgg cacgggcgtt gttgatcgac aggccgatcc cgaccgccat
      361 caggaacggg acccagcgcg cgatcccacc ccgccgaccc gtcgcctcgc cgtgggccaa
      421 cacgtagaac gcggcgaccg acagcgagct gaaggtgaac aacggaagat cgacggcgac
      481 gatccaccag ggggagatct cggcgcgcag ataaacggcc ggcacgacca acagcgccag
      541 caggaccatc agcacgtaac ccacgttcgc ggtcaggtgg acggccgatt cgagcttgac
      601 gcggaacggc aggcggccgg tcaggatcgc cggcaacagc ttgcgcgccg tctggatcga
      661 gcccttggcc cagcggtgct gctgcgactt gaacgagccg atctcgatcg gaagctccgc
      721 gggtgcgacg tgctcgttga cgaagacgaa gcgccagccc ccaagctgcg cgcggtagga
      781 caggtcgaga tcttcggtca acgtgtcgtg ctgccagcct cccgcgtcga cgatgcactc
      841 gcggcgccag attcccgcgg tgccgttgaa gttgaagaag cggccggagc ggttgcgcgc
      901 cgtatgctcg atgacgaagt gtccgtcgag caacatcgat tgaacgcggg tcagcggcga
      961 gtgatccgcg ttgaggtgcc cccagcgtgc ctgcaccatc ccgacccgcg ggtcgacgaa
     1021 gtggtggacg agctgtcggg cgaagtcggg ccgcggcacg aagtccgcgt cgaacaccgc
     1081 gatcagctcg cccttggccc ggcgctgacc ggtctcgagc gcgccggcct tgaagccgcc
     1141 gcggtcggtg cggcaaaggt gcacgatgtc gaacccttcg ccgactttct gctcgacctt
     1201 gcgacgcacc agcgccgtgg tctcgtcggt cgagtcgtcg agcacctgaa tctcgagcct
     1261 gtcgcgcggg tagtcgagcg cgcagaccgc gtcgatcagg cgctcgacga cgtacatctc
     1321 gttgaacaac ggcagctgca cggtcagcgg cggcaacggc tcgagccgac cgggaggcac
     1381 caccgcccgg tcgcgatggc gcaggtagag cgcgacgagg taccagcggt ggatcccgta
     1441 gagcgtcagg actcccagac aggcgaagta gagccccaac gtcccccagc gcagtgcgac
     1501 caccaactcg tcagccatcc gcgcccggcc tccgcatctt ccccgcgcgt gctaggctgg
     1561 cgccgccgtg agccacgccc gcccgtcgag tccgccgacc gattccaacc ggcggaaggc
     1621 ccggatgatt cccgtgctcg ccgtcctctc cgccgcgatc tacggcgcgc tggcgttcgc
     1681 cggcgacctg acccagcgtc cgacggtgtt gtacggcggc tgcgggttgc tgctggcggc
     1741 gatgtgggcc gcttggtgtc tgtcgcgcgg tggtgcgacc cgtctccgtc cgatcctggc
     1801 cgcggcgctc ctcttccgct tgatcgccgc ctgcggtcct cccgccctgt cggacgacgt
     1861 ctaccgctac gtctgggacg ggcgcgttca gttgcacggt atccacccgt acctcttcgc
     1921 gcccaccgac ccgcgcgtgg aagcgctgcg cgacgagaac tggcggcgga tcaaccatcc
     1981 gcaactgatc acgatctacc ccccggtctc ccagttgctg ttcgcggccc tcgccgcgtc
     2041 cggcgccggg ccgggcgggt ttcgcatcgc gctcggtctg gtcgacttcg gcgtcgtgct
     2101 ggccctgtcg ctgctgctgc gccgcctcgg gttgccgggt gaacgcgtgg tgctctacgc
     2161 gtggaaccct ctcgcggtgc tcgaagcgtc gggcagcggc cacgtcgaac cgctgggaat
     2221 cgccttcgtg ctgctcgcca ccgcctggat tataggttgc cggcaccggc tgtcaacggc
     2281 ggcgctcgcg gcggcgattc acgtcaagct gctgccggtc gtgctgatcc cggtcttcgc
     2341 gccgagctac cgtatccgcg gcgtcgtctg gctcgtgctg tggctgctcg tgctgtgggc
     2401 cccgtacggc ttgagcggtc ccgcgttcgg cgccggtctg tgggcctacg ccgaatcgtg
     2461 ggagcacaac tcgtcgatct tcgccctcgt cgagaccgcg ctcgtcgcgc tcgacagcgg
     2521 ccggctgctc gaaccgctcg tcagctcggt caaggagcgc agcggcgccg cctgggtccc
     2581 gtgggacagc ctctaccgct ggctctggcc gccggggctg gcccgcgcgc tggtcgccgg
     2641 cgccgggatc gtctgggtcg tcgcggtcgc gcgtcgcgac gggatcccgc tcgcgcggag
     2701 atgcctcctc gcgatcggcg gcgtgctgct gctctcgccg acgctgcacc cgtggtacgt
     2761 gctgtgggtg cttccgttcg ccgccgccct tctctcgtgg ccgtggctcg tccttgccgc
     2821 gctcgtgccg ttgagctatc tcggcgccgg cgccgacccg ccaccgtggg tcctgctcgt
     2881 cgagtacggc atgccgtgcg ggatcgtcgt cgtcgatcgg ctggcgcggc gttcgacgag
     2941 ctatgctgtc gatcgatgaa cgtgctcgac gccatcctgt cccgcgcccg gcgcgacccc
     3001 gtgcgcatcg cgatgcccga gggcgaggac ccgcgcgtct tgcgcgccgc gcttcgcctg
     3061 gcacgcgagc ggatcgccac cccgcttttg gtcggggaag cgcagacggt gcgcctcgcg
     3121 gccgagcggg catcggtcga gctgggcccg gtcgcgatcg tcgaaccggg ccgacacgcg
     3181 gcgcacgacc gctgcgcgca cgccgctcgc gaggcgctcg ctccccgcgg tgcgacggcg
     3241 gaggaagtcg aacgatgcct cggcgacccg ttgtactacg cggcgtcgat ggtgcgcgcc
     3301 ggcctggccg acggtacgct ggccggcgcg acgcactcga cggcggagac gttgcgcgcg
     3361 gcgctgcgga tcatcggacc cgcacggtcc accggaacgg tctcctcctt cttcttgatg
     3421 gcgttgcgcc agccgacgag tgcgggcgac gacgtgctgg cattcgccga ctgcggcctg
     3481 atcccgtatc ccgacgccgc ccagctcgcc acgatcgccc gacagaccgc cgcgcagttc
     3541 cggctgctcg tcgaacgcga gccgcgcgtg gccctgctgt cgttctcgac ggcgggcagc
     3601 gcgtcgcatc cgtcggtcga cagcgtgcgt gccgcgcgcg acctgctcgc ggcgggcgac
     3661 gccgacttcg agttcgacgg tgagatgcag gtcgacgcgg cgctggttcc cgacgtcgcc
     3721 cgggccaagc tggcccgcag cccggtcgcc ggccgggcca acgtgttgat cttccccaac
     3781 ctcgacgcgg gcaacatcgc ctacaagctg gtcgagcggc tggccggagc caccgcgctg
     3841 ggcccgctcg tgcagggcct ggcgcggccg ttcatggacc tctcgcgcgg ctgcaagccc
     3901 gacgacatcg tcagcgtagc ctgcatcgcg tcggcgctcg ccgactgagc gccggtccgg
     3961 cgcagcgcgt
//
LOCUS       DASPGN010000493         6839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1026941, whole genome shotgun sequence.
ACCESSION   DASPGN010000493 DASPGN010000000
VERSION     DASPGN010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026941"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="VD788_14335"
     CDS             complement(<1..531)
                     /locus_tag="VD788_14335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77489.1"
                     /translation="METIQVQDRVPLGRQVPLVPALEQRLQTRDDPRDRALRQVLSTQ
                     QRLERLANAPGVGTREVAPQDRLIDGLGPAGVARQQLAAKLGRPASLADPTTRDSNLA
                     RADLGGHQPALHTVAVAAPTFAALVALASEGLGELFLEHHLDGLLYPLPQPGLDVQVE
                     LDDSALTRSRLFPHGVP"
     gene            692..1216
                     /locus_tag="VD788_14340"
                     /pseudo
     CDS             692..1216
                     /locus_tag="VD788_14340"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2055..2613
                     /locus_tag="VD788_14345"
                     /pseudo
     CDS             2055..2613
                     /locus_tag="VD788_14345"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(2938..5343)
                     /locus_tag="VD788_14350"
     CDS             complement(2938..5343)
                     /locus_tag="VD788_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV77490.1"
                     /translation="MARRRALIVDRDRGFSARLRGCLRALGMVVDVAETRRETLDRLA
                     AKPPSIVFIAVERPKKTGFSLFSDVKRALKNVPIVLTTASVPMGEMMLHQKLRLHAEL
                     YLDKRGITEAEILATLSGLLRLKLDSSDLAELASTVSRAVALSADRGDDDDEQEDYRP
                     YLSDAYDAAADPPTDAADEDEETGAADDDDGDLDPPPSAAHERADRSLELNQRLHSLT
                     RELERARRAAGSSPFSKEYLELGDALERAEQRLLRLETEVEHARRENDALRTKLIETT
                     RRRTEAERARDEAIDRARDLEQAGDDLRLRVERETEANRAHAEKLAEETGQRVRLEQD
                     HAFAVEKLRERNERERGDAVAQAVAEATQRHELDQARLAAQHAEALAAAERSAEQRLV
                     AEREGLREQAQRATDALHREHAAELARLRDEAVHERQALERQHDERLERTVREHQQAL
                     AAAERRHAELLADGARLADEQTARGLDGRDREWHERLDRLREQHATQLERLRRELVEE
                     RTSLTDKLYLDRDSAVTLRDAEWEEKLERLRADHAGRLDEIRMQHADKLAELTRAHQT
                     DLTQRDREHARAIERLTGELHRERTRSSGERSDDLVRSESEHAAEIERVEARHQDEIA
                     VLERVHHEQLQDLRAKQTSERERSTAAHTAALDELSERLRRDQRVTLEGKESDWQQRL
                     ERLRDEYDTALEAEREQLRTVLEALGEARAAAERTGETGSPTGAVDDGDDVHEERTGR
                     IYRILGRTTSRVSKPGRASQLLRHLAAELDEIGGAGAAEIDMPDVDAAFREDDRPRRG
                     EDA"
     gene            5698..6393
                     /locus_tag="VD788_14355"
     CDS             5698..6393
                     /locus_tag="VD788_14355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77491.1"
                     /translation="MAKQKPEVAPMWTRAAVYQVENKILQQIMSARTPEATEAAVSYA
                     RFLRLSGLNVDNYPLFLKMLEIENHWVLDELIGPGDPFLLLSTIPPNRYLTQKAFQLL
                     TRWRPGGIYPKTLAIVLGVLQNTYSSPKDGYKIYPLSVADVDNLGKHLEKQKGQNDPR
                     NRCILDIMDRIGSLEGLYWDQSMEHVARQALRIRTNFFDNTKILEDCMPQVLLVQGDY
                     SQDEVAPRRVFAG"
     gene            6406..>6839
                     /locus_tag="VD788_14360"
     CDS             6406..>6839
                     /locus_tag="VD788_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77492.1"
                     /translation="MSEAVLQLETVDRREAESVVHERQIFETESRLLQGIFGCRSTEA
                     VESAITYARFLNSVGITPDNYPVFLKMLEIANHWVIDALIGSRDPFLLLSPVQPNRFI
                     VERCLAMMTRWHKGGIYSKNLSVIMGVLQSVYSSPRDGYRIAP"
BASE COUNT         1041 a         2304 c         2343 g         1151 t
ORIGIN      
        1 gggtacgccg tggggaaaga gtctactacg agtgagggcc gagtcgtcca gttccacctg
       61 gacatccagg cccggttgcg gcagcgggta caggaggcca tcgaggtggt gctcgaggaa
      121 gagctcgccg aggccctcgg atgcgagagc taccagcgct gcgaaggtcg gcgcggctac
      181 cgcaacggtg tggagcgccg gctggtgacc accgaggtcg gcccgcgcga ggttcgaatc
      241 cctcgtggtc gggtccgcca ggctggcggg acgaccgagt ttcgcagcga gctgctgccg
      301 cgctacgccc gccggaccaa ggccgtcgat gaggcgatcc tggggagcta cctcgcgggt
      361 gccaacaccc ggcgcattcg caaggcgctc gagccgctgc tgggtactga gcacctgtcg
      421 aagagcgcgg tctcgcgggt cgtcgcgcgt ctgaaggcgc tgttcgagag ctggaacgag
      481 cgggacctgt cggccgagcg gtacgcgatc ctgtacctgg atggtttcca cctcaaggtc
      541 cggctggccc ggcgagtgat ctcggtgccg gtgctggccg tgctcggggt ggccgaggac
      601 ggcacgaagg tgctggtgtc gttgaagccg gccgcgagcg aggccgccag ccactggtcc
      661 gcggtgctgc tcgacctgca aggacgccgg gctctcggca ccccggctgc tcgtcgtcga
      721 cggccacgag ggtctgaaca aggcgctgaa ggtctggtcg ggggttcagg ttcagcgctg
      781 tgcccgtcac aagtggatca acctgcgcga ccactgcccg gtccacgcgc ggacggagtt
      841 gaagcgcgac taccacggga tcatcaacgc caaggacggc atggccggac gggaggcgta
      901 cgacgcgttc ctgaccaagt ggtcgaagct ctgcccggcg gtggcgcgct cgctggaaga
      961 agccggtctc gagctgctta ctttctacca gtaccccaag gcgatgtggc gctcgctgcg
     1021 cacgaccaac ccgttggaga atctgaaccg ggagtttcgc cgacggacca agacgcaggc
     1081 gtcgttcgcc aacgaagacg cggggttgac cgtgctgtac ggcctggttg ccttcgagca
     1141 gatccgcttc cacaagatcg acggccatcg ccacgtcgcg gggctgctcc gcgcgaagga
     1201 gacgcacgcg gcatgaaggc cttggtcggg tcgaatatag ccgctcgggc actcgcggcg
     1261 aggggtgcaa cgaaaaccga gcgcgtagga gccacgcaga gcgaggatcg cgagggtggc
     1321 catacgatcg gacccccctc gaaatcgtct caggcagctt cgtgtatcgc gacccgatca
     1381 tcggcattga cgacaccaga tcggacgagg tacataaact cagcagaggc tacgccagaa
     1441 cttttccacg gaaccaggga cacaaccttc gatcgcgctg tgtgatgctg atagtgggta
     1501 tccggacgcc cccaactcct gctcgcttcc ggacgtggaa aggtcacttg gtggattcag
     1561 agcgaatgtc tacgccgctc cgcgtctcga tggacgagca tcaccggcga ggtggtcgag
     1621 atggcgcttc gaactgagat caccgggctc gactttctct gcttcccttg atgaagaatc
     1681 gcaggttgat gtgcgtctgg gggaagagga ataaggaggc tgtcgtcgat ccgcgcagcc
     1741 taatcgctcc ttcggccgac gtggccctac gcgcgctacg gcgcgctccg ggaccacgca
     1801 gccgaagggg cgattagcct gacgcggccc cccggggcac gccgcgcgcc ggccgccgat
     1861 gaacgcttct gctgaaccgt gaccctcgcg ggcaaggtcg cgctggtgac gggcgggtcc
     1921 cgcggcatcg gcgccgcgat cgcggggcgg ctcgcgctgg aaggcgccga tgtcgccatc
     1981 acctccgcga gcgcggcgga gaaggcggcc gtggtgcgca ggccgtcgag gcggccggcg
     2041 cgcgggcccc gtccatccgg gccgacagcg cggacgccgc ggcggtgaag ggcgccgtcg
     2101 acgagacggt gcatgccctc ggacgcctgg acatcctcgt caacaacgcg gggatcgcgg
     2161 tgatcaagcc gctcgacgag ctctcgatcg aggacttcga ccgcatgacg gcggtgaatg
     2221 tccgcgccgt cttcgtggct gccagggagg cggcccgcca catgggcgag ggtggccgca
     2281 tcatcaacat cgggagcgtc aacgcggcgc gtgccgttcg tgggcggcag cgtctacgcc
     2341 atgaccaagg cggcggtcgc cggactcacc cgtgggctcg cccgcgacct cggcccgcgc
     2401 ggcatcaccg tcaacaccgt ccagcccggc cccgtcgaca ccgacatgaa cccggcggag
     2461 ggccccttcg ccgagacctt gaaggccctc atggccctgc cgcgctacgg gcgcggcgac
     2521 gaggtcgccg ccatggtcgc ctacctcgcg gggcccgagg cgggtttcgt caccggcgcc
     2581 tgtctgacca tcgacggagg gttcggcgcc tgacatggct cccgtgtcca cccctgaccc
     2641 cccgcagcag gtcgaacgcg tcgagggact gttggttgcc cggctcgatc ggcgtgtcct
     2701 tgagggaggt ggctctgagg gccgcgcgga gcccaggcct gtcaccggtg agccagcggg
     2761 ccaaggaagg ggagtatcga gcgcttctgg accattacgc cgcgcccagg ctgtgccgtt
     2821 ggcgcgtccc cgccacgaag cgttcccggg gatccagccg aattgtacgg cttccgagcg
     2881 ggcccccgct accgaaccga cagttcggcg gcttctccgg ggcttctcac agcgggctca
     2941 ggcgtcttcg ccgcgacgcg gccggtcgtc ctctcggaac gccgcgtcga cgtcgggcat
     3001 gtcgatctcg gccgctcccg cgcctccgat ctcgtcgagc tcggcggcca ggtgacgcag
     3061 tagctggctg gcccggccgg gcttcgaaac gcggctcgtg gtgcggccga ggatacggta
     3121 gatgcggccg gtccgttcct cgtggacgtc gtcgccatcg tcgaccgcgc ccgtcgggct
     3181 gccggtctca ccggttcgct cggcggcggc tcgcgcctcg ccgagggcct cgagcacggt
     3241 gcgcagctgc tcgcgctcgg cttcgagcgc ggtgtcgtac tcgtcgcgca accgctcgag
     3301 ccgttgctgc cagtcggact ccttgccctc gagcgtcacg cgttgatcgc gtcgcagccg
     3361 ctcgctcagc tcgtcgagag ctgccgtgtg cgcggccgta ctccgttctc gctcggaggt
     3421 ctgcttggcg cgcagatctt gcagctgttc gtggtgaacc cgctcgagca ccgcgatctc
     3481 gtcctgatga cgcgcctcga cgcgctcgat ctcggcggcg tgctcgctct cgctgcggac
     3541 caggtcgtcc gaccgctcac ccgacgaacg ggtccgctcg cgatgcagct ccccggtcag
     3601 ccgctcgatc gctcgcgcgt gctctcggtc gcgctgggtc agatcggttt ggtgggcccg
     3661 cgtcagctcg gccagcttgt cggcatgctg catgcgaatc tcgtcgagcc ggccggcgtg
     3721 gtcggcgcgc agccgctcga gcttctcctc ccactccgcg tcgcgtagcg tgaccgcgct
     3781 gtcgcgatcg agatagagct tgtccgtcag gctggtgcgc tcctcgacga gttcgcgccg
     3841 cagccgctcg agctgcgtgg cgtgctgctc gcgcaggcga tcgagccgct cgtgccactc
     3901 gcggtctcgg ccatcgagac ctcgcgcggt ctgctcgtcg gccagccgag cgccatcggc
     3961 cagcagctcg gcgtggcggc gctcggccgc ggcaagcgcc tgctgatgct cgcgcaccgt
     4021 tcgctcgagg cgctcgtcgt gttgccgctc cagcgcctgg cgctcgtgca cggcctcgtc
     4081 gcgcaaccgc gcgagctcgg cggcgtgctc gcggtgcaac gcgtcggtcg cgcgttgcgc
     4141 ctgctcacgc agaccctcgc gctcggcgac cagacgctgc tcggccgatc gttccgcggc
     4201 ggcgagcgct tcggcgtgtt gggcggcgag acgggcctga tcgagctcat gacgctgcgt
     4261 cgcctcggcc acggcctgcg ccacggcgtc gccacgctcg cgctcgttcc gttctcgcag
     4321 cttctcgacg gcgaacgcgt ggtcctgctc caggcgcacc cgctggcccg tctcctccgc
     4381 gagcttctcg gcgtgcgcgc ggttcgcctc ggtctcgcgc tcgactcgca accgcagatc
     4441 gtcaccggcc tgctccaggt cgcgggcccg atcgatcgcc tcgtcgcgag cgcgctcggc
     4501 ctcggtgcgc cggcgggtcg tttcgatcag cttggttctc agcgcatcgt tctctcgccg
     4561 ggcgtgctcg acctcggtct cgagccgcag cagtcgctgc tccgcgcgct cgagcgcgtc
     4621 gcccagctcg aggtactcct tcgagaaggg cgagctgccg gcggcgcgtc gtgcgcgttc
     4681 gagctcgcgg gtcaagctgt gcaagcgctg gttgagctcg agcgaacgat cggctcgctc
     4741 gtgggcggcg gacggcggcg gatcgagatc gccgtcgtcg tcgtccgcag cgccggtctc
     4801 ttcgtcttcg tcggccgcgt cggtcggcgg gtcggcggcg gcgtcgtacg catcggacag
     4861 gtaggggcgg tagtcttcct gctcgtcgtc gtcgtcgccc cgatcggccg acagcgcgac
     4921 cgcgcgggag acggtcgagg cgagttcggc aaggtcgctc gaatcgagct tcaaccggag
     4981 cagcccgctc agcgtggcga ggatctcggc ctcggtgatc ccgcgtttgt cgagatagag
     5041 ctcggcatgg agccggagct tctgatgcag catcatctcg cccatcggga cgctggcggt
     5101 cgtcagcacg atcgggacgt ttttcaaagc gcgcttgacg tcggagaaca gcgagaagcc
     5161 cgtcttcttc ggtcgctcga cggcgatgaa gacgatcgac gggggttttg ccgcgagtcg
     5221 atcgagcgtc tcgcgacgcg tctcggcgac gtcgacgacc atgccgagcg cgcgaaggca
     5281 gccgcgcagt ctcgcggaga agcctcggtc ccgatcgacg atcagcgcac gccgacgtgc
     5341 cacgccctct ccctgtcgcc gccgacggag ctcgttgccg gatcgatctg cgagttcaac
     5401 ggcacctatc tttagtttct tggtacggac tctcgaccgg tccgggcgac gcaggccgac
     5461 cctgcccgga gcgagggatg aataggttcc tcgccaaacc gtgtcaagcg gatcgggaaa
     5521 acccggaaag accggaaaac cgcggtggcg gcacgtcacc ttgacaatcg tcgtgacgga
     5581 cctaagtttg cctccgtttc agttgacgag atcgtttcgt ccgtgccggt gagcgtcacc
     5641 accgggcgat ggcgcggtcg agcggtcccc aaccaaccat cgcggaggag gcaccccgtg
     5701 gccaagcaga agccggaggt cgcgccgatg tggacccggg cagcggttta ccaggtcgag
     5761 aacaagatcc tgcagcagat catgtcggcg cgtacgccgg aggcgacgga ggccgcggtc
     5821 tcctacgcgc gcttcctgcg cctgtccggg ctgaacgtcg acaattaccc gctgttcctc
     5881 aagatgctcg agatcgagaa tcactgggtg ctggacgagc tgatcggacc gggagacccg
     5941 ttcctgctgc tgtccaccat cccgcccaat cgctacctca cgcagaaggc gttccagctg
     6001 ctgacgcgat ggcgtcccgg tggaatctat cccaagacgc tggcgatcgt gctcggcgtc
     6061 ctgcagaaca cctacagttc accgaaggac ggctacaaga tctacccgct gtcggtcgcc
     6121 gacgtcgaca acctcggcaa acatctcgag aagcaaaagg gccagaacga cccgcgcaac
     6181 cgctgcattc tcgacatcat ggaccgcatc ggctcgctgg aaggcctgta ctgggaccag
     6241 tcgatggagc acgtcgcccg tcaggcgttg cgtattcgga cgaacttctt cgacaacacg
     6301 aagatcctcg aagactgcat gccgcaggtg ctgctggtgc aaggtgacta cagccaggac
     6361 gaggtcgcgc cccggcgcgt cttcgccggt tgaggagcga acgagatgag cgaagccgta
     6421 ctgcaattgg aaaccgtcga ccggcgcgag gccgagagcg tcgttcacga acgacagatc
     6481 ttcgagaccg aaagccgact gctgcaaggc atcttcggtt gtcgcagcac cgaggcggtc
     6541 gagtcggcga tcacctacgc tcgcttcctc aacagcgtgg ggatcactcc ggacaactac
     6601 ccggtcttcc tcaaaatgct cgagatcgcc aatcattggg tgatcgacgc gctgatcggc
     6661 agccgcgacc cgttcctgtt gttgtcgccg gtccagccca atcggttcat cgtcgagcgc
     6721 tgcttggcga tgatgacccg ctggcacaag ggcggcatct acagcaagaa cctgtcggtc
     6781 atcatgggcg tgctccagtc ggtctacagc tcgccccgcg acgggtatcg gatcgcgcc
//
LOCUS       DASPGN010000494         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_208448, whole genome shotgun sequence.
ACCESSION   DASPGN010000494 DASPGN010000000
VERSION     DASPGN010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208448"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VD788_14365"
     CDS             <1..853
                     /locus_tag="VD788_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXV77493.1"
                     /translation="ETRLLHHKVRVQWNHRLESVETKDGGIVAQVAHLDRVATGYPIA
                     GAEWMVTKTLAIHAKYVVGADGYHSRIREELGLTYEGLGPLQTFSVFEFETASDPGFE
                     LAVVLDGESVSACWPMSDRRCRWSFQVPSAERHDSTIVRLNELIKARAPWFPEVRGEL
                     LWASMVQFDRRLASGLGAGRLWLAGDSVHMTTPVGVQSMNAGLVDAHELSWRLADRVR
                     GERGDDVLASYSAERSRELARLVRPDATAAPAGAPAWVRRHWGAIGAAIPATGDQLDA
                     LLAPLEA"
     gene            complement(817..1722)
                     /locus_tag="VD788_14370"
     CDS             complement(817..1722)
                     /locus_tag="VD788_14370"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV77494.1"
                     /translation="MIRPRAEITPARVADHYDELDPIYRRIWGDHVHHGLWETGRESP
                     ALAVRRLVGRVAGAGGFRPGDSVCDIGCGYGATARELARNHRLRVTGITLSAVQFDYA
                     SRRAARDARVEIRLGDWLANDFADRSFDGAIAIESTEHIADKERCFAEMRRVLRPGGR
                     VVICAWLASDEPRNWHVRHLLEPICREGRLAGMATAREYRAWLERAGLDPIGFDDLSD
                     RVRRTWTICIRRFLGALPRDRALRRYLWAATSRNRVFAATRFRIRLAYAAGAMRYGLF
                     VAVRPSDATPHAAGQASSGANSASS"
BASE COUNT          314 a          720 c          762 g          321 t
ORIGIN      
        1 cgagacccgc ctgctgcatc acaaggtccg ggtgcagtgg aatcaccggc tggagagcgt
       61 cgaaacgaag gatggcggga tcgtcgcgca ggttgcccat ctggaccgtg tggcgaccgg
      121 ctacccgatc gcgggtgccg agtggatggt gaccaagacg ttggccatcc acgccaagta
      181 cgtcgtcggg gcggacggtt accactcgag gatccgcgag gagctcggtc tgacctacga
      241 gggcctcggg ccgttgcaga ccttctcggt gttcgagttc gaaacggcgt ccgaccccgg
      301 tttcgagctc gcggtcgtgc tcgacggcga gtcggtcagc gcctgttggc cgatgtccga
      361 ccgccgctgc cggtggagct tccaggttcc ttcggcggag cggcacgaca gcacgatcgt
      421 gcgtttgaac gagttgatca aggctcgcgc gccgtggttc cccgaggtcc gcggcgagct
      481 gctgtgggcc tcgatggtcc agttcgaccg ccgcttggcc tccggcctgg gtgccggccg
      541 cctgtggctc gccggcgatt ccgtgcacat gaccaccccc gtcggtgtac agagcatgaa
      601 cgccggtttg gtcgacgcgc acgagttgtc ctggcgcctc gccgaccgtg tccgcggcga
      661 gcgcggcgac gacgtactgg cgtcctactc cgccgagcgc tcgcgggagc tcgcacggct
      721 cgtgcgaccc gacgcgacgg cggctccggc gggggccccc gcctgggttc ggcggcattg
      781 gggcgcgatc ggtgcggcga tcccggcgac cggtgatcag ctcgacgcgc tgttggcgcc
      841 gctcgaagcc tgaccggcgg cgtgcggcgt ggcgtcgctc gggcggaccg cgacgaacaa
      901 cccgtagcgc atcgcgcccg cggcgtaggc caagcggatg cggaaccggg tcgccgcgaa
      961 gacgcggttg cgagaggtcg cggcccacag gtagcgtcgc agcgcgcggt cgcgcggtaa
     1021 ggcgccgagg aaccgccgga tacagatcgt ccacgtccgg cggacccgat cgctgagatc
     1081 gtcgaacccg atcgggtcga gccccgcccg ttcgagccag gctcgatact cgcgcgccgt
     1141 ggccatcccc gccagccgcc cttcgcgaca gatcggttcc agcaggtgcc ggacgtgcca
     1201 gttccgcggc tcgtccgacg cgagccaggc gcagatcacg acgcggcccc cgggccgcag
     1261 cacgcggcgc atctcggcga agcaacgctc cttgtccgcg atgtgctcgg tgctttcgat
     1321 cgcgatcgct ccgtcgaacg aacggtcggc gaagtcgttc gccagccaat cgccgagtcg
     1381 gatctcgacg cgcgcgtccc tcgccgcccg ccgcgaggcg tagtcgaact ggacggccga
     1441 gagggtgatc ccggtcacgc gcagtcgatg gttgcgcgcc agctcgcgcg cggtcgcccc
     1501 gtagccgcaa ccgatgtcgc agaccgaatc acccggccgg aatccccccg ccccggcgac
     1561 cctgccgacc agacggcgaa cggcgagcgc gggcgattcg cgaccggtct cccacagacc
     1621 gtgatgcacg tgatcgcccc agatccgacg gtagatcggg tccagctcgt cgtagtgatc
     1681 ggcgacgcgg gcaggcgtga tctcggctcg gggtcggatc aaatccgtcc cgtttcggca
     1741 gcggaccgat tttctcgttg acaaatcacc gagcgcagcc tactacaagg gacgaagatg
     1801 atggcgcttc gtcagaccgg ctggtggtgg tggacctcga cacgggaggc cggtggctga
     1861 cgtctctatc agaagacccg acagagcgac caaaaagccc cggcgttgcc ggggcttttt
     1921 cttttggcgt ccgaggagga cgatgggcga gcgcggcggg cggttgatgc agacggcgat
     1981 cgcgcgggcg gtcgaggggc acgacctcgg gctggaacgg gcccggcgcg tcgcctcgca
     2041 catcatcgcc gggcgggcca caccggccca gctcggcggt ctgctggtcg cgctgcgggc
     2101 caagggcgag accgtcg
//
LOCUS       DASPGN010000495        10109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1144807, whole genome shotgun sequence.
ACCESSION   DASPGN010000495 DASPGN010000000
VERSION     DASPGN010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10109
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144807"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VD788_14375"
     CDS             <1..1446
                     /locus_tag="VD788_14375"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXV77495.1"
                     /translation="VEAGKEALLGFELVPNARTVVLGTVPADVAVSLDGEPVGLTATS
                     TDDGGIGEPEARLVIEDVPLGEHVFELSKECFRTERLSDNLNVDLLTREPKHYKTVRM
                     VPARTQVTLRGGPSDGQLTIDDRPAGRLSGEPVSACPGDRTFVVSVGGRVVWQHDVTL
                     RDAVDEVLEVAPRPNLVRVGTERWPRGLEAFGAAFNLLGERELPRGRDLLQADAWEEM
                     RLPDAVDLALAVVPPSRAGAPDRWYLYSPILDRAVLVDGDPPDPSRPQWTAVTWGFDV
                     VDSEIGGANVVVGVEAGGTAATAGMKVGDRLVEIAGRAVASAAETRATLANARPEAPL
                     RVAWTTAAGERREEELTGRVGPLLVVPAAESAAALVRAAWAAVDTLTRPELAAAAMAN
                     LALLFEAAGQPAAAVDAWRRVDWGDRPGVGEGTRQYYLGRALVRLGREAQALEALRKA
                     AATRATAFSDDGPPIAPAAADHLADLGTTPD"
     gene            complement(1443..2087)
                     /locus_tag="VD788_14380"
     CDS             complement(1443..2087)
                     /locus_tag="VD788_14380"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV77496.1"
                     /translation="MACNEWNEQWVARLYDELDPDEDRALSTHLADCDACRATLDELE
                     RSRLLLGRTSPYVPAGPRVIVLGRPRGRRAAWNFAYGVAAAWVVFVAGAWVGTQWLGS
                     APADAGVDAVLATSAAERAALENRIGELEARLTEVASSERVGDATAAPVTREEHRAAL
                     AKLEREIERQRARDMEFVMGEMSAVEFRAAKWVDETREGVRLVAMQNDPNLSQR"
     gene            complement(2108..2692)
                     /locus_tag="VD788_14385"
     CDS             complement(2108..2692)
                     /locus_tag="VD788_14385"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV77497.1"
                     /translation="MRGVTDEDLVQAVQAGDTDSLGVLVSRWEQPLYRFIYRMLPRRE
                     DAHDVCQETFLRILRRADRYRPGSRFSTWMYQIALNLCRDQARKRKRWGLILAERHEF
                     DERQPGLENGSTGAADPAASVERDEMTRAVRRAMEQIPQEQREVLIMKEFEGLKFREI
                     AEILGCPESTVKSRMYYGLSGLRQVLVRSGIHHP"
     gene            3065..3220
                     /locus_tag="VD788_14390"
     CDS             3065..3220
                     /locus_tag="VD788_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77498.1"
                     /translation="MTREEIDERLQAVASRVAAQGGEQPPVDPSEPRWIRPADLVRRM
                     LPYLVYN"
     gene            3221..3586
                     /locus_tag="VD788_14395"
     CDS             3221..3586
                     /locus_tag="VD788_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HXV77499.1"
                     /translation="MTRSRLDDRSSSGPTDGLAKRRAELERLDAAIERVLQDQRETWE
                     RMAALSHKINNPLTSLIGRAQLLRHRHGNDEYVRHAAEVIEESSRRVADYLRELTGLV
                     KERQQEIVRLSRESSGAND"
     gene            complement(3583..4611)
                     /locus_tag="VD788_14400"
     CDS             complement(3583..4611)
                     /locus_tag="VD788_14400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HXV77500.1"
                     /translation="MRIPVPLAWLFLVITFFTAGLLRQYHDRTPTSPYVNPVAGSLLF
                     VSCFFLLLVSAREWRRGAVGGQGVRLGSITPIMLMLLIEKWVSLTLYEPLFAWLAPRD
                     EPARLLDARFRGLAGVALILVCLLVGRFSIPTARKTWRRARPSRWPVGAAVTAAVVGG
                     GYALLGGLAYLLGGVFRLRWPDADALLWSVLWSQAVLAFAEEVYYRGLLLSEMERLAP
                     RLGLRNPLGRRWAALLFTAGLFSLEHVRADSPTGEAPRYLVFTLALGLLLGLLVMVSA
                     NLHLTAGVHVWINWVVLGAAPVFVDEAGEPALPAGTYIGVTLILAFAMVYLQQRLRRR
                     RHGLPTKL"
     gene            complement(4608..5534)
                     /locus_tag="VD788_14405"
     CDS             complement(4608..5534)
                     /locus_tag="VD788_14405"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HXV77501.1"
                     /translation="MPIALIVHGGAWNIPDAGVEASREGVRRVLVDGWEALRRGAPAI
                     DVVETILRALEDDPHFDAGRGSRLNREGKVELDAALMLGTNLEAGAVAAVQGVRHPIS
                     VARLVMERSQHVMLAGEGARKFAREAGAELCRTRDLLVGREYERYMRVLAGEQALVDE
                     EFHDGVTDRPEGTVGAVALDRERRIVAGTSTGGTQHKLPGRVGDTPIIGAGTYADDLI
                     GGASATGWGEGILRVALTKAAVDYMEEGLDPAGAGRRALDALRRVNGRAGLILLDRQG
                     RVAGVFNTPRMARGWATENDGPRVAIEADEGS"
     gene            complement(5607..8888)
                     /locus_tag="VD788_14410"
     CDS             complement(5607..8888)
                     /locus_tag="VD788_14410"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXV77502.1"
                     /translation="MRAGIGPFITLGALLLSPAADAAIDARMLRFPDVSREQIAFVYA
                     GDIWIVGKSGGVAHRLSTPAGQETFPRFSPDGRSIAFSGNYDGNTDLYVVPASGGIPL
                     RLTHHPSNDRMLDWYPDGESILFASSMASGTNRFNQLYRTSRTGGLPERLPVPYGEFG
                     SVAPDGRRLAYLPLTRDFRTWKRYRGGSTSDIWLFDLERRTADNLTDSRSNDTQPMWH
                     GETLYFLSDRDANKRYNIWAIDGAGGSPRQVTRFDTMDVAFPAIGPAEIVFSVGSELY
                     LLDLDSERYAAVDVEVVTDRATLKPRHTEVHEQITSGDISPTGKRAVFEARGEIFTVP
                     AEHGVTRNLTRSSGVAERHPAWSPDGEWVAYHSDRSGEYELTIAAADGSGDERRLTTL
                     GPGYRYDLHWSPDSKKLVFIDQTMTIRLYDLDKETTTALDKGRYLYHGGLSGFVARWS
                     ADSRWVAWARSVDTRNRAIFVYDAREGELHQATSGFYDEQEPVFDPEGKQLYFLSSRT
                     FEPAYSDIDNSWIYANTANVVAVPLRDDVASPIAPRNDDEGQDESDDDAKKDKKKDKK
                     QEKDDEQDEKKPPQPVEIDFDGFERRLVVLPVDPGNFATLEAVPGKVVYHRRPRTGAD
                     EEAPSPIVYYDLEEREEKTILEDADDFAISADDEKMLVVHEEKFAIVDVAAEQKMDET
                     LDLSGLELELDPVAEWRQIFHDAWRFERDYFYDPRMHGVDWDDMRERYAALLDDVVTR
                     WDLNFLIGELIAELNASHAYRGGGDEQQPKELGTGLLGCDFELVDGAYRIARIVDGAV
                     WDSEVRSPLARPGIEIDEGDYLLAVNGRPIDTTKDPWASFQGLADATVELTVNDRPTT
                     EGARQVLVHTLDSEFRLRNLDWIETNRRKVDAATGGRVGYVYVPDTGINGQNELVRMF
                     KGQYAKDGLIIDERFNSGGQIPDRFIELLNRPLYNYWGVRDGKDWQWPPVAHRGPKAM
                     LVNPWSGSGGDLFPYYFKQAGLGPLVGMTTWGGLIGISGSPALIDGGVVTVPTFGIYS
                     LDGRWIIENEGVEPDIEVVDDPSKMVDGGDPQLERAIAEVMERLRDAPVTEPGKPPYP
                     DRSGR"
     gene            complement(8981..9967)
                     /locus_tag="VD788_14415"
     CDS             complement(8981..9967)
                     /locus_tag="VD788_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXV77503.1"
                     /translation="MRQTDARWAVVLGAGDGTRLHALTGRGGVIVPKQFCLLQDDSLI
                     DLALRRAERVVCRARITVVVAARHRWWWRHDLRRLPERNVIVQPKNRGTAPGILLPLL
                     SILARDERASIVLLPSDHYVDDEATLQASIEQAFDEIERDPRGIVLLGITPDGPETDY
                     GWIVPGERLAPQTSSVVEFVEKPAPAAARRLLERRAAWNSFVIVARVDALLRLYAERA
                     PELLGRLAGAVTPSGEVDPMAARRVYAELTARDFSRDLLHGSERSLRLVAVPPCGWTD
                     LGTPERVATCAQRLTGERTTAERPSSRVDLLSALSRFGEDSSRVPAARSAGG"
BASE COUNT         1529 a         3615 c         3417 g         1548 t
ORIGIN      
        1 gtcgaggcgg ggaaagaagc gctgctcggg ttcgagctgg tgccgaacgc acgaacggtc
       61 gtcctcggca cggtgcccgc cgatgtcgcg gtgtcgctcg acggcgagcc ggtcggtctg
      121 accgcgacct ccaccgacga cggtggaatc ggcgagccgg aagcgcggct ggtgatcgag
      181 gacgtgccgc tcggcgagca cgtcttcgag ctgagcaagg agtgcttccg caccgagcgg
      241 ctgtcggaca acctgaacgt cgacctgctg acccgcgagc caaagcacta caagacggtg
      301 cggatggtcc ccgcccgcac gcaggtcacg ttgcgtggcg gtccgagcga cgggcagctc
      361 acgatcgacg accggccggc gggacgcctg tcgggcgagc cggtttcggc ctgcccgggt
      421 gaccgcacct tcgtcgtcag cgtcggcggg cgggtcgtct ggcagcacga cgtcacgctg
      481 cgggacgcgg tcgacgaggt gctcgaggtc gctccgcgtc ccaatctcgt gcgggtcggg
      541 acggagcgct ggccgcgggg tctcgaagcg ttcggggccg cgttcaatct gctcggcgag
      601 cgcgagctgc cccggggtcg ggatctgctg caggccgacg cctgggagga gatgcgactg
      661 cccgacgccg tcgatctcgc cctcgccgtc gtgccgccgt cgagggccgg tgcccccgac
      721 cggtggtatc tctacagccc gatcctcgac cgcgcggtac tggtcgacgg cgaccctccc
      781 gacccgtcac ggccgcagtg gacggcggtg acgtggggat tcgacgtggt cgactccgag
      841 atcggcgggg cgaacgtcgt cgtcggcgtc gaggccggcg gaaccgcggc gacggccggg
      901 atgaaggtcg gtgaccgact ggtcgagatc gccggccgcg ccgtcgcgag cgccgccgag
      961 acgcgtgcca cgctggccaa cgcccgaccg gaggcgccgt tgcgcgtcgc gtggacgacg
     1021 gcggcgggcg agcgcaggga agaggagctg accggccgcg tcgggccgct cttggtcgtg
     1081 ccggccgccg aatccgctgc agcgctcgtg cgcgcggcgt gggctgccgt cgacacgctc
     1141 acgcggccgg agctcgccgc ggcggcgatg gcgaacctgg cgctgctgtt cgaggcggcc
     1201 ggccagccgg cagcggcggt cgacgcgtgg cgccgggtcg actggggcga ccggcccggt
     1261 gtcggcgagg gaacgcgcca gtactacctc ggccgggcac tcgtgcggct cggtcgcgag
     1321 gcccaggcgc tcgaggcctt gcgcaaggcg gccgcgacgc gcgccacggc gttctccgac
     1381 gacgggccgc cgatcgcccc ggcggctgcc gaccatctcg ccgacctggg cacgacgccc
     1441 gactagcgct ggctcagatt cgggtcgttc tgcatcgcga cgagacgtac gccttcgcgt
     1501 gtttcgtcga cccacttggc ggcgcggaac tcgacggcgc tcatctcccc catgacgaac
     1561 tccatgtcgc gcgcacgctg gcgctcgatc tcgcgctcga gcttggccag cgccgcccga
     1621 tgctcctcgc gcgtcaccgg cgccgcggtc gcatcgccca cgcgctcgct ggacgcgacc
     1681 tcggtcagtc tcgcttcgag ctcgccgatg cggttctcga gcgccgcgcg ttcggcggcg
     1741 gaggtggcca gcaccgcgtc gacgccggcg tcggccggtg ccgaacccag ccactgcgtg
     1801 ccgacccacg ccccggcgac gaagacgacc catgcggcgg ccacgccgta ggcgaagttc
     1861 caggcggcgc ggcgcccacg gggacgcccg aggacgatca cccgcggacc ggcgggcacg
     1921 tacggcgagg tccgcccgag cagcagccgc gaccgttcga gctcgtcgag cgtcgcgcgg
     1981 cacgcgtcac aatcggccag gtgggtcgac agcgctcggt cttcgtccgg atccagttcg
     2041 tcgtagaggc gggccaccca ttgttcgttc cattcgttgc acgccatgac gtcctctcct
     2101 ccacgcatca ggggtggtgg atgccgctgc ggaccagcac ctggcgcagc ccgctgagac
     2161 cgtagtacat ccgcgacttg accgtgctct ccgggcaacc gaggatctcc gcgatctccc
     2221 tgaacttgag tccttcgaat tccttcatga tcagtacttc gcgctgctct tgcgggatct
     2281 gttccatcgc ccggcgcacc gctcgcgtca tctcgtcccg ctcgacggag gccgccggat
     2341 ccgccgcgcc ggtcgagccg ttctcgagcc ccggctgtcg ctcgtcgaac tcgtgtcgct
     2401 cggccaggat caacccccag cgcttgcgtt tgcgggcctg gtcacggcac aggttcagcg
     2461 cgatctggta catccaggtc gaaaagcgcg aacccggccg ataccgatcg gcccgccgca
     2521 ggatacgcag gaaggtctcc tgacaaacgt cgtgcgcgtc ctcgcgacgc ggcagcatgc
     2581 ggtagatgaa ccggtagagc ggttgttccc atcgcgaaac gagcaccccc agcgagtcgg
     2641 tgtcccccgc ctggaccgcc tgcaccaggt cttcgtccgt cacccctctc acgcgagatc
     2701 gaccctccac cctggtgaaa cgtccggatg gaatgagaag acgcccggat ggcgagaatt
     2761 gtaccggtgc gggaagcgct cggcggtggt cacccagcgt cggtcaaaat ctgtcacgac
     2821 gttcgcccac cgcggccaat tagtgtcgcg gttccgcacc gcgcggaccg tcgcccgccg
     2881 cgcctcgggc gatcgattca cccgaatccc gaacgctggt tgcatatccg aaatgtggct
     2941 cgcctattgc tagagtcgtt gacttgcata tttcgaaagg acgcggcaac cgtcgaccgc
     3001 gtcgaggggg ttcccgatgg ccgcagatcg ttctacccga atcgctcccg atcgcttgag
     3061 gccgttgacc cgagaagaga tcgacgagcg gctccaggcc gtcgcctcgc gagtcgccgc
     3121 ccaaggcggc gagcaaccac cggtcgatcc ctccgagccg cgctggatcc ggcccgcgga
     3181 cctcgtgcgc cggatgctcc cctacttggt ctacaactag atgacccgtt cgaggctcga
     3241 cgaccgatcc tcgtccgggc ccaccgacgg gctcgcgaag cggcgcgcgg agctggagcg
     3301 cctcgacgcg gcgatcgagc gggtgctcca ggaccagcgc gagacgtggg agcggatggc
     3361 tgccctcagc cacaagatca acaacccgct gacctcgctg atcggccggg cacagttgct
     3421 gcgccatcgt cacgggaacg acgagtacgt ccgtcacgcg gccgaggtca tcgaggaatc
     3481 gtcgaggcgg gtggccgact acctgcgcga attgaccggt ctggtcaagg aacgccagca
     3541 agagatcgtg cggctgtcac gcgagtcgtc cggcgccaac gactagagtt tcgtcggcag
     3601 tccgtgccgc ctgcgccgca gccgctgctg caagtagacc atcgcgaaag ccaggatcaa
     3661 cgtcacgccg atgtaggtcc ccgccggcag cgccggctca ccggcctcgt cgacgaagac
     3721 cggcgcggcg ccgagcacga cccagttgat ccagacgtgg acaccggcgg tcaggtgcag
     3781 gttggccgac accatcacga gcagaccgag cagcagcccg agcgccagcg tgaacacgag
     3841 gtagcgcggc gcctcgccgg tcggcgagtc ggcccgcacg tgctccaggc tgaacagccc
     3901 ggccgtgaac aacagcgcgg cccaccgtcg tccgagcggg ttgcgcaggc ccaggcgcgg
     3961 cgccagccgc tccatctcgc tcagcagcaa accgcggtag tacacctcct cggcgaacgc
     4021 cagcaccgcc tggctccaca gaaccgacca gagcagggcg tccgcgtccg gccagcgcag
     4081 gcggaacacc ccgcccagca ggtaggccag accaccgagc agcgcgtagc cgccgccgac
     4141 gacggccgcg gtcaccgcgg cgccgaccgg ccagcgcgac ggacgcgcgc gacgccaggt
     4201 tttgcgcgcg gtcgggatcg agaagcggcc gacgagcagg cagaccagga tcagggcgac
     4261 gccggccagg ccgcggaacc tggcgtcgag cagccgcgcc ggttcgtccc gcggggcgag
     4321 ccaggcgaac aacggctcgt agagcgtcag cgagacccac ttctcgatca gcagcatcag
     4381 catgatcggc gtgatgctgc ccagcctgac gccctggccg cccaccgcac cgcgccgcca
     4441 ttcccgcgcc gacacgagca acaggaagaa gcacgagacg aacagcaggc tgccggcgac
     4501 cgggttgacg tagggcgagg tcggtgtccg gtcgtggtac tgacgcagca ggccggcggt
     4561 gaagaaggtg atgaccagga acaaccaggc cagcgggacc gggattctca actgccttcg
     4621 tccgcctcga tcgcgacccg cgggccgtcg ttctcagtgg cccagccacg cgccatgcgc
     4681 ggcgtgttga agacgccggc gacgcgcccc tggcggtcga gcaggatcaa gccggcccgg
     4741 ccgttgactc gacggagcgc gtcgagcgcc cggcgcccgg cacccgcggg atcgagtcct
     4801 tcctccatgt agtcgaccgc ggccttggtc agcgccaccc gcaggatccc ttcgccccag
     4861 ccggtcgccg acgcaccgcc gatcaggtcg tcggcgtagg ttccggcgcc gatgatcggc
     4921 gtgtcgccga cccggccggg gagcttgtgc tgcgtgccgc cggtcgacgt gccggcgacg
     4981 atccgccgct cgcggtcgag ggcgacggcg cccaccgtgc cctcgggtcg gtcggtcacc
     5041 ccgtcgtgga actcctcgtc gaccagcgcc tgctcgcccg cgagcacacg catgtaacgc
     5101 tcgtactcac gcccgacgag caggtcgcgt gtgcggcaca gctcggcccc cgcctcgcgc
     5161 gcgaacttgc gcgcgccctc gcccgcgagc atcacgtgct gcgagcgttc catcaccagc
     5221 cgcgccacgc tgatcggatg gcgcaccccc tggaccgccg caacggcgcc ggcctcgagg
     5281 ttggtcccga gcatcagcgc cgcgtcgagt tcgaccttcc cctcccgatt cagacgcgag
     5341 ccgcgcccgg cgtcgaaatg cgggtcgtcc tcgagcgccc ggaggatcgt ctcgacgacg
     5401 tcgatcgccg gcgctccccg gcgcaacgcc tcccaaccgt cgaccagcac ccgccgcacg
     5461 ccttcgcgcg acgcttcgac gccagcgtcc gggatgttcc acgccccgcc atggacgatc
     5521 agtgcgatgg gcaccctcgc gctcctccgc cctcaaggta cgaacgcgta gtatatcggc
     5581 ccaccgcgat cgcggcggcg agcggcctag cggccgctgc gatccgggta gggtggtttg
     5641 cccggctcgg tcaccggggc gtcccgcagg cgctccatca cctcggcgat cgcccgctcg
     5701 agctgcgggt cgccgccgtc gaccatcttc gacggatcgt cgacgacctc gatgtcgggc
     5761 tcgaccccct cgttctcgat gatccaccga ccgtccaggc tgtaaatgcc gaaggtcggt
     5821 accgtcacca cgccgccgtc gatcaacgcc ggggacccgc tgatcccgat cagtccgccc
     5881 caggtggtca tcccgacgag cggtccgagc ccggcctgtt tgaagtaata cgggaacaga
     5941 tcgccacccg aaccgctcca cgggttgacc aacatcgcct tcggtccgcg gtgcgcgacc
     6001 ggcggccact gccagtcctt cccgtcgcgc acgccccagt agttgtagag cggccggttc
     6061 agcaactcga tgaaacgatc cggtatctgg ccgccgctgt tgaagcgctc gtcgatgatc
     6121 agcccgtcct tggcgtactg gcccttgaac atgcgcacca gctcgttctg gccgttgatc
     6181 ccggtgtcgg gcacgtagac gtagccgacc cgtccccccg tcgccgcgtc gaccttgcgc
     6241 cggttcgtct cgatccaatc gagattgcgc agacggaact cgctgtccaa cgtgtgaacc
     6301 agcacctgcc gcgcgccctc ggtcgtcggc cggtcgttga cggtcaactc gaccgtcgcg
     6361 tcggccagcc cctggaacga agcccacggg tccttcgtcg tgtcgatcgg tcttccgttg
     6421 accgccagca ggtagtcacc ctcgtcgatc tcgatccccg gccgggcgag cggcgagcgg
     6481 acctcgctgt cccagaccgc gccgtcgacg atccgcgcga tgcgataggc cccgtcgacg
     6541 agctcgaagt cgcagccgag caggcccgtg ccgagctctt tcggctgctg ctcgtcgcct
     6601 ccgccgcggt aggcgtgcga ggcgttgagc tcggcgatca gctcgccgat gaggaagttg
     6661 aggtcccaac gcgtgacgac gtcgtcaagc agcgccgcgt agcgctcgcg catgtcgtcc
     6721 caatcgacgc cgtgcatgcg cgggtcgtag aagtagtcgc gctcgaagcg ccaggcgtcg
     6781 tggaagatct gacgccactc ggccaccgga tcgagctcca gttcgagccc cgacaggtcg
     6841 agcgtctcgt ccatcttctg ctcggccgcg acgtcgacga tggcgaactt ctcctcgtgg
     6901 acgaccagca tcttctcgtc gtcggccgaa atggcgaagt cgtccgcgtc ttcgaggatc
     6961 gtcttttcct cgcgctcttc gagatcgtag tagacgatcg ggctcggcgc ctcctcgtcc
     7021 gcgccggtac gcgggcgacg gtggtagacc accttgcccg gtaccgcttc gagcgtcgcg
     7081 aagttcccgg gatcgaccgg caggacgacc aagcggcgct cgaatccgtc gaaatcgatc
     7141 tcgaccggct gaggtggctt tttttcgtcc tgctcgtcgt ctttttcctg tttcttgtcc
     7201 ttcttcttgt ctttcttcgc gtcgtcgtcc gactcgtcct gcccctcgtc gtcgttgcgc
     7261 ggcgcgatcg gcgaggcgac gtcgtcgcgc agcggcaccg cgacgacatt ggccgtgttg
     7321 gcgtagatcc agctgttgtc gatgtcgctg tatgcgggtt cgaacgtccg gctcgagagg
     7381 aagtagagct gcttgccctc cggatcgaac accggctcct gctcgtcgta gaaccccgac
     7441 gtcgcctggt gcagctcgcc ctcgcgagcg tcgtagacga agatcgcgcg gttgcgcgtg
     7501 tcgacggagc gcgcccacgc cacccagcgg ctgtcggcag accagcgtgc gacgaagccc
     7561 gacagaccac cgtgatacag gtagcgtccc ttgtcgagtg cggtcgtcgt ctccttgtcc
     7621 aggtcgtaca ggcggatcgt catcgtctga tcgatgaaga ccagcttctt gctgtccggc
     7681 gaccagtgga ggtcgtagcg gtagcccggc cccaacgttg tcaggcggcg ctcgtcgccc
     7741 gagccgtcgg cggcggcgat cgtcagctcg tactcgccgg accgatcgct gtggtaggcg
     7801 acccactcgc cgtcgggcga ccacgcggga tgacgctcgg ccacgcccga gctccgggtc
     7861 aggttgcggg tcacgccgtg ctcggccggc acggtgaaga tctccccgcg cgcttcgaac
     7921 accgctcgct tgccggtcgg cgagatgtca ccgctcgtga tctgctcgtg gacctccgtg
     7981 tgacgcggtt tgagcgtcgc gcggtcggtg acgacctcga cgtcgaccgc cgcgtaacgc
     8041 tcgctgtcca ggtcgagcag gtagagctcg ctgccgacgg agaacacgat ctccgcgggg
     8101 ccgatcgcgg gaaacgccac gtccatcgtg tcgaagcgcg tcacctggcg cgggctgccg
     8161 ccggcgccgt cgatggccca gatgttgtat cgcttgttcg cgtcgcggtc cgacaggaaa
     8221 tagagcgtct cgccgtgcca catcggctgc gtgtcgttgc tgcgggagtc ggtcaggttg
     8281 tcggccgtcc gccgttcgag gtcgaacaac cagatgtccg aggtcgaacc gccgcgatag
     8341 cgcttccacg tcctgaaatc gcgggtcaac ggcaggtagg ccagtcgcct gccgtcgggc
     8401 gccacgctgc cgaactcacc gtagggtacg ggcaaccgct cgggtagccc gccggttcgc
     8461 gaggtgcggt agagctgatt gaaccgattc gtcccgctgg ccatcgacga agcgaacagg
     8521 atcgactcgc cgtccgggta ccagtcgagc atccggtcgt tcgacgggtg atgcgtcaac
     8581 cgcagcggga tcccgcccga cgccgggacg acgtagaggt cggtgttgcc gtcgtagttg
     8641 ccgctgaacg cgatcgagcg gccgtcggga gagaaccgcg gaaaggtttc ctgaccggcc
     8701 ggggtgctga gtcgatgggc caccccgccg gacttgccga cgatccagat atccccggcg
     8761 tagacgaacg cgatctgctc ccgcgagacg tcgggaaagc gcagcatccg agcgtcgatc
     8821 gcggcgtcgg ccgcgggcga tagcagtaac gcaccgaggg tgatgaacgg accgattcct
     8881 gctcgcacga ttctctctcc tccgccggag tctcccggat ccgccagagg atgccacgat
     8941 ccgcggcgcg aggaaagccg tcgcgccgga ttccgcctgg ctagccaccc gccgagcggg
     9001 ccgccgggac tcgcgagctg tcttcgccga agcgagacag cgcgctcaac aggtcgacgc
     9061 gcgacgaagg gcgctcggcc gtcgtccgct cgccggtcag tcgttgcgcg caggtggcga
     9121 cccgctcggg cgtgcccaga tcggtccagc cgcacggcgg gaccgccacc aggcgcagcg
     9181 agcgctccga gccgtggagc aggtcacggg agaaatcgcg ggctgtcaac tcggcgtaga
     9241 ctcgccgggc ggccatcgga tcgacctcgc ccgacggcgt gaccgccccg gccaagcggc
     9301 cgagcagctc cggcgcgcgc tcggcgtaga gccgcaggag cgcgtcgacc cgggcgacga
     9361 tcacgaagct gttccacgcc gcccggcgct ccaacaatcg ccgcgcggcg gccggcgccg
     9421 gcttctcgac gaactcgacg accgacgacg tctgcggcgc gagtcgctcg ccggggacga
     9481 tccaaccgta gtcggtttcc ggtccgtcgg gcgtgatacc gagcaggacg atgccgcgcg
     9541 ggtcgcgctc gatctcgtcg aacgcctgct cgatcgacgc ctgcaacgtg gcttcgtcgt
     9601 cgacgtagtg gtcggacggc agcagcacga tcgaggcgcg ctcgtcccgg gccaggatcg
     9661 acagcaacgg cagcagaatt cccggcgccg tcccccgatt cttcggttgg acgatgacgt
     9721 tgcgctcggg cagccgccgc aggtcgtgtc tccaccacca acgatgccgc gcggccacga
     9781 cgacggtgat ccgcgcccgg cagaccacgc gctcggcacg ccgcagcgcc aggtcgatca
     9841 acgagtcgtc ctgcaggagg cagaactgct tcggcacgat gacgcccccg cgtccggtca
     9901 gcgcgtgcag gcgagttccg tccccggccc cgagcaccac ggcccatcgc gcatcggtct
     9961 gtcgcatcgg tcgctcctcc tcgggcgttt ccgtcggctg tggtggatcg gatgccgcgc
    10021 acggcgcgcg gctttcgcgg agcgcgacgg ccgctcgctg aaacgtcgcg cggcatcgcg
    10081 gaatccaacg cgtcgaactc gcccgtacg
//
LOCUS       DASPGN010000496         2743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_208648, whole genome shotgun sequence.
ACCESSION   DASPGN010000496 DASPGN010000000
VERSION     DASPGN010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208648"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1885)
                     /locus_tag="VD788_14420"
     CDS             complement(47..1885)
                     /locus_tag="VD788_14420"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXV77504.1"
                     /translation="MRRRDSLVLSLALAAAGCSGGGGEALDGRLRGAATGYNVLLVTL
                     DTTRADRLGCYGGEWVETPALDALAASGVVFAEAVTVAPVTAPSHASMLTGLYPPSHG
                     LRTNGQFRLDERRLTLAELLRSRGYQTAAFVSAFVLDHRFGLDQGFELYDDAVRPKTR
                     VGPEEIQERSATEVTDAALGWLGRRTADRPFFAWVHYFDAHVPYAPPLEFAARYPNRP
                     YAGEIAYVDSQLGRLVAALDEQGLRERTLIFVIGDHGESLGDHGELTHGFFVYDSVMR
                     VPFIVSAPALFAGPRVVDDAVASVVDLLPTVADMLGIDEIPPLDGSSLLRGGGSTERA
                     VYMESMNPYFDHGWSPLMALRGASDKYIRAPRAEYYRLDADPAEQDNLVGRAPAGVGR
                     SIDRRASALGEMLERWPSVERAADDAIPLDPESLARLQSLGYVGGLPDGDEATFADPK
                     DKIGEYERFQRASFLASTGREHEALAILEELIAAAPDDRLVLDQLAQANVFLGRLEPA
                     EAYVRRLLAIGEDADSLMLLAQVLIAQDRLDEVDPLTERALALDPEHGTSIVVQGDLA
                     ARRGRFVEAAERYRQAARVDPYRVGPLVESRMAWLARLRSEAGVGG"
     gene            complement(1996..2502)
                     /locus_tag="VD788_14425"
     CDS             complement(1996..2502)
                     /locus_tag="VD788_14425"
                     /inference="COORDINATES: protein motif:HMM:NF041384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HXV77505.1"
                     /translation="MNRVRSLRSSVLPALAVLSVALAACGVTPAEQGAAPRVLVNVDE
                     NGVALQGYDPVAYFTDGKPVKGRAEINATHEGAIYHFASAEHRAMFVDDPAHYAPQYG
                     GYCGYAASINKISPIGPEWFQVIDDRLILQHNKKAWTLWNEDLPGNIVKADANWPGLV
                     SRHGTASP"
     gene            complement(2499..>2743)
                     /locus_tag="VD788_14430"
     CDS             complement(2499..>2743)
                     /locus_tag="VD788_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77506.1"
                     /translation="PRTAIYGALLTLGVIGGAIATHLFVVGIEIVDPATGAGDRGTLF
                     GLAIVVAVLASIVFWVRKETRVGLARLPGRRRGGMS"
BASE COUNT          448 a          985 c          931 g          379 t
ORIGIN      
        1 cggccgcccg acaagttcgc cgcggcaacc acgtcacgcc gaccggtcaa ccgccgaccc
       61 cggcctccga gcgcagccgc gcgagccagg ccatccgcga ttcgaccaac ggacccacgc
      121 ggtacggatc gacccgcgcc gcctgccgat accgttcggc ggcctcgacg aatcgcccgc
      181 gtctggcggc gagatcaccc tggacgacga tcgacgttcc gtgctcgggg tcgagcgcga
      241 gcgcgcgctc ggtcaacgga tcgacctcgt cgagccggtc ctgcgcaatc agcacctggg
      301 ccagcagcat cagcgaatcg gcgtcttcgc cgatcgcgag caggcgtcgc acgtaggcct
      361 cggccggctc gagccggccg aggaagacat tggcctgggc cagctggtcc agtacgagcc
      421 ggtcgtcggg cgcggccgcg atcaactcct cgaggatcgc cagcgcttcg tgctcgcggc
      481 ccgtcgacgc caggaacgac gcccgctgaa accgctcgta ctcgccgatc ttgtccttgg
      541 gatcggcgaa ggtcgcctcg tcgccgtccg gcaggccgcc gacgtagccc agcgattgca
      601 agcgcgcgag gctctccgga tcgagcggaa tcgcgtcgtc ggccgctcgt tcgacggacg
      661 gccagcgctc gagcatctcg ccgagggccg aggctcgccg atcgatcgac cgccccaccc
      721 cggcgggggc ccggccgacc aggttgtcct gctcggcggg atcggcgtcc agccgatagt
      781 actccgctcg cggcgcccgg atgtacttgt cgctcgcgcc gcgcagcgcc atcaacggcg
      841 accagccgtg gtcgaagtac gggttcatac tctccatgta gaccgcgcgc tcggtcgagc
      901 ccccgcctcg gagcagactg ctgccgtcga gcggggggat ctcgtcgatg ccgagcatgt
      961 cggcgacggt cggcaacagg tcgacgaccg acgcgaccgc gtcgtcgacc acccgcgggc
     1021 cggcgaacag tgccggcgcc gagacgatga acggcacgcg catcaccgag tcgtagacga
     1081 agaagccgtg cgtcagctcg ccgtggtcgc cgagcgactc gccgtgatcg ccgatgacga
     1141 agatcagcgt gcgctcccgc aggccctgct cgtccagtgc ggcgaccaac cggccgagct
     1201 gcgagtcgac gtaggcgatc tcaccggcgt acggccgatt cgggtagcgc gccgcgaact
     1261 cgagcggcgg cgcgtagggc acgtgcgcgt cgaagtagtg aacccaggcg aagaacggcc
     1321 ggtcggcggt gcgccgaccc agccaaccca gcgccgcgtc ggtgacctcg gtcgccgagc
     1381 gctcctggat ctcctccgga ccgacccgcg tcttgggccg gaccgcgtcg tcatacagct
     1441 cgaagccctg atccaggccg aagcgatgat cgaggacgaa ggccgagacg aacgccgcgg
     1501 tctggtagcc ccggctgcgc aacagctcgg ccagcgtcag ccggcgctcg tcgaggcgga
     1561 actgcccgtt ggtccgcagg ccgtggctcg gggggtacag accggtcaac atcgaggcgt
     1621 gggacggggc ggtgaccggg gcgaccgtca ccgcttcggc gaacaccacc ccgctcgcgg
     1681 ccaacgcgtc gagcgccggc gtctcgaccc actcgccgcc gtagcagccc aaccgatcgg
     1741 cgcgcgtcgt gtcgagcgtc acgagcagaa cgttgtaccc ggtggccgca ccgcgcaaac
     1801 gcccgtcgag cgcctcgccg cctccgcccg aacaaccggc ggcagcgagc gcgagcgaca
     1861 gcacgagcga gtcgcgacgg cgcatgcgcg ccgcaggttg cgacggggag attgcgggcc
     1921 ggagcgccat acagcgattc tacgggtcga tcgccgaggc cggcaagcgc ggtacaggtc
     1981 gccggggacg gctcgtcacg gactcgctgt gccgtgtcgg ctgaccaacc ccggccaatt
     2041 cgcatcggct ttaacgatgt tgccgggcag atcttcgttc cacagcgtcc acgccttctt
     2101 gttgtgttgg agaatcagcc ggtcgtcgat cacctggaac cactcgggcc cgatcggcga
     2161 gattttgttg atcgacgcgg cgtagccgca gtagccgccg tattgcggcg cgtagtgcgc
     2221 cggatcgtcg acgaacatcg ctcggtgctc cgccgaggcg aagtggtaga tcgcgccctc
     2281 gtgggtcgcg ttgatctcgg cgcgcccttt gaccggcttg ccgtcggtga agtaggcgac
     2341 cggatcgtaa ccctgcagcg ccaccccgtt ctcgtcgacg ttgacgagca cacgcggcgc
     2401 tgcgccttgc tcggcggggg tgaccccgca ggccgccagc gcgaccgaca acactgcgag
     2461 cgccggcagg accgaactgc gaagggaccg aacacgattc atgacatgcc tcctcgacgg
     2521 cggccgggca atcgagccag accgacccgt gtttcctttc tgacccaaaa tacgatgctt
     2581 gccagcacgg cgaccacgat cgccagaccg aacaacgtgc cgcgatcccc ggccccggtc
     2641 gccggatcga cgatctcgat gccgacgacg aacaggtgcg tggcgatcgc cccgccgatc
     2701 acaccgaggg tcagcagcgc gccgtaaatc gccgtgcgcg gag
//
LOCUS       DASPGN010000497         8212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_91987, whole genome shotgun sequence.
ACCESSION   DASPGN010000497 DASPGN010000000
VERSION     DASPGN010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91987"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..804
                     /locus_tag="VD788_14435"
     CDS             340..804
                     /locus_tag="VD788_14435"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HXV77507.1"
                     /translation="MGDREREDERGGPTEDSGVKVTDRRLFTSDGSLRQEIDEEDEGE
                     QGRPAGRGEPPVGGATGFERRSLDEPAAVDFTMLINAMAQPALIFLGEVAHPGSGREE
                     IDLEQARLQIDLLDVLRIKCRGNLSPEEDALLDRVLYELRMRYLARSNQASR"
     gene            884..2938
                     /locus_tag="VD788_14440"
     CDS             884..2938
                     /locus_tag="VD788_14440"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77508.1"
                     /translation="MKRPSRVIRGALLLAVCWAWSPDSRTQSDDGVIRLRPRPVADPA
                     AGAALDPAPAGANSAAVDMTGPRRGFDYHAFETRLESLWFQRKTLVADGRVDDASEQS
                     ELIKAFCIEEGVHHLDDLAGALVAEAERYVEQGHYERAVDALALAEVFDPGRAQVHLA
                     RADLHWQAGHGSAAAARELLLGLRHAVASSLREMSLFSRVLLVALVAAVGCFAVFSLS
                     MLVRYQIPFRHEVEEWVRHNVGERWARALGWFVLCLPLATWAGAGWVALYWVLITIRF
                     MRPAERLTAFLLLIVTALALPLYRTAVGTYGVTADPVVRTTLQSAEGEYDPDTILSLR
                     KLVQAHPNDPVYRFLLAGLYKNGRYYEDAFAEYKEALALDPALEQAHINIGNIFFATG
                     QYAEAIASYNEAIAIDPRSVLAYFNRHLAQSESFRFREAEATIEEARAIDADALSELF
                     ATADVDGERRTVAEASLGMGSVWQAALGGGADGSGVTNPAALELAAVARQFANPISAA
                     AVTVALACMLLLMAGRAGTPARRCIRCGRPFCHRCNTRRDSHEYCSQCVHLFVLGDGL
                     AAETKTRKLYEVGRHERQSRGARSAVSLVLPGAAQLLRGQAARGVLLVGAWLAAWIAW
                     RPQLLEPLDRLAGVDVRIDLMQQPSVPAGFQPQPLALLALVSLVVVWLSGNAWRWRKR
                     EG"
     gene            2945..4201
                     /locus_tag="VD788_14445"
     CDS             2945..4201
                     /locus_tag="VD788_14445"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXV77509.1"
                     /translation="MALQGTLKDFGLGDIFQLIGIQRKTGILALDNGDDSVTVKFLEG
                     QVVGADTKRRSVEELLGSVLVRTGRISQSQLQDALKRQKRTLQRLGYVLVQSGLISED
                     DLIEALRVQSLQIIYRLFRWRDGSYSFRGVEDLEYDEKHFIPISAETILMEGARMIDE
                     WPIIERRIKSDRIVLRATDAAASLGLTVEAIVDRDIGIDPNHGAEGGVRLSREEREVL
                     GLVDGRRTVEEINGFSTLGEFDTYRVLSELMTRNLIEEVKRVPVRPVGGIHVRRLGDR
                     LFRWTLDLIVLGVVVAGVASLRANPWVPWRIDSLRATTEQLRLHASQARLEHLESAIQ
                     VYYLDRGVFPQSLELLAANRYVRPGETRDPWGRPYGFRLSASGYQLYGWDGAGELRPN
                     LTLARRFTAIQRMMSGLAEAEPSANE"
     gene            4362..6287
                     /gene="dnaK"
                     /locus_tag="VD788_14450"
     CDS             4362..6287
                     /gene="dnaK"
                     /locus_tag="VD788_14450"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXV77510.1"
                     /translation="MGKIIGIDLGTTNSVVAVMEGGKPTIITNPEGSRLTPSVVAIND
                     KGERLVGQVAKRQAITNPTNTVYSIKRFMGRKFSEVSHEAREVAYDVLAADNGDVRVK
                     IRDKMYAPPEISAMVLRKLKEAAEAHLGERVDAAVITVPAYFNDAQRQATKDAGQIAG
                     LKVERIVNEPTAAALAYGLEKKTDETIAVFDFGGGTFDISILEVGEGVVEVKATNGDT
                     HLGGDDLDQRLIEWIVAEFKKDQGIDLSQDKMALQRLKEAAEKAKCELSTTSETEINL
                     PFITADANGPKHLQLKLNRAKFEQLVGDLLDKTLEPCRRAMSDASLEPSDIDEVVLVG
                     GSTRIPRVQQMVQELFQREPHKGVNPDEVVAVGAAVQAGVLGGDVKDILLLDVTPLTL
                     GIETLGGVFTKLIQRNTTIPTRKSEIFSTAADGQTSVEVHVLQGEREMAAYNKTLGKF
                     HLTGIPPAPRGVPQIEVTFDIDANGIVNVSAKDLGTGAEQKITITASSGLSGDQVEKL
                     VKDATVHAAEDREAREAVDARNKCDQLVYQVEKLLKDNDAKLSERDQAPVRDALATAK
                     KALEAGNVGEIRAATERLEKASHQLAEAIYRSRADAGVSTADEATAGARGSSGSGGNG
                     HGEVIDAEVVDSDSSGR"
     gene            6318..6770
                     /locus_tag="VD788_14455"
     CDS             6318..6770
                     /locus_tag="VD788_14455"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXV77511.1"
                     /translation="MKKGADWNPLSELLGVQKRMNQLFESALTRSEIEAHDGLGVWIP
                     VCDVYENAGGTLVVGIELPGIDRRSIDVRIDDDELVIEGCREMDREREGEHFHRVERS
                     YGKFSRRFRLPSKIDRESVTAAYKDGVLTVTLARRDGAKPGPIQVSVS"
     gene            6891..7748
                     /locus_tag="VD788_14460"
     CDS             6891..7748
                     /locus_tag="VD788_14460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HXV77512.1"
                     /translation="MEREREETRSSGSESLKKYLKEISRLPRITVQEERDLGARIQKG
                     DTKALRRLVEANLRFVVSYAKRYRGCGLSFLDLINEGNIGLIEAAKRFDPDKGVKFIT
                     YAVWWVRQAIIHALSDQSGAFRLPQKQANLLYRIGKAQANLRIELKRTPSTEEIARKM
                     EVTVEEVNNLLQVSDDNISLSAVIDEEHDFHLSDKLEQETIPSADLVMLKNSLRGLLR
                     NALTELDPKEEQVLRLRFGLDGTDPKTLKEIGEMLSLSRERIRQIEAQALEKLNRSHK
                     CQQLRGYLN"
     gene            7790..>8212
                     /locus_tag="VD788_14465"
     CDS             7790..>8212
                     /locus_tag="VD788_14465"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV77513.1"
                     /translation="MAAECPNCHGTGFELRSSQNGVITSKRCACDLARLGERLLRRAR
                     IPPRYEHCTFDEFRIRPDDESHAHALATAREWVGLWPTVTSGLLFLGPPGTGKTHLAV
                     AIARELAGAKNADVLFYEQRELMKSLQATFESGSSQRES"
BASE COUNT         1505 a         2616 c         2783 g         1308 t
ORIGIN      
        1 atcgcgtcgg ccgcttcgta gcattcctcg atcaagtagc tgcgaagcgt gtcgaacgtc
       61 tgttcacggt cccaggggca gccgtcgggc ccccgcaagc gctccatgat cccaaccaaa
      121 gtggcgaagc gcgacagctg cttcacggcg ttactcctgc ttgacttgac catcggccga
      181 acacatcttt gcaaccggcg tggggatctc ccgttggggc cgaaaggtct cgccaacgcc
      241 caccggaagg cgcgtggcgg ggtgtttgaa accccggaaa cccgaatggt accatccggg
      301 gcttgtcacg agccgcggaa cggccgaacg ggggagacca tgggagacag ggaacgagaa
      361 gacgaacgag gaggcccgac ggaggacagc ggagtcaagg tcacggaccg gcggctgttc
      421 acgagtgacg gtagcttgcg gcaggaaatc gacgaggaag acgaagggga gcaggggcga
      481 ccggccggcc gcggcgaacc gccggtcggc ggggcgaccg gcttcgagcg acgctccctc
      541 gacgaaccgg cggcggtcga tttcacgatg ttgatcaacg ccatggccca acccgcgttg
      601 atcttcctcg gcgaggtggc gcacccggga tccggccgcg aggagatcga cctcgaacag
      661 gcgcgtctcc agatcgacct gctcgatgtc ctgcggatca agtgtcgagg gaatctgtca
      721 cccgaggagg atgccctgtt ggaccgcgtt ctctacgagc tgagaatgcg ctacctcgcg
      781 cgctccaacc aggccagccg ctgatcccgg tcccggaccg gtcggtacgt tcctcggcga
      841 cgcgttggca cgtggcgatc cgcaccgacc ccggggacga acgttgaagc gaccgagccg
      901 tgtgatccga ggcgcgctgc tgctcgccgt ttgctgggct tggtcgcccg acagccggac
      961 ccaatcggac gacggcgtca ttcggctccg ccctcgaccg gtcgccgacc cggccgccgg
     1021 cgccgcgctc gatcccgcac cggcaggggc caattccgcc gcggtcgaca tgaccggacc
     1081 gcggcggggc ttcgactatc acgccttcga gacgcggctc gaatcgttgt ggttccaacg
     1141 caagacgctc gtggccgacg ggcgcgtcga cgacgccagc gagcaatccg agctgatcaa
     1201 ggcgttctgc atcgaagagg gggtgcacca tctcgacgac ctggccggcg ccctcgtcgc
     1261 ggaggccgaa cgctacgtcg agcagggtca ctacgaacgt gcggtcgacg ccctggcact
     1321 ggccgaggtg ttcgaccccg gtcgagcaca ggtgcacctg gcacgggccg atctgcactg
     1381 gcaggcgggc cacggcagcg ccgcggccgc ccgcgagctg ctgctcgggc ttcgtcacgc
     1441 cgtggcgagc tcgctgcgcg agatgagcct gttcagccgc gtgctgctcg tcgccctggt
     1501 tgccgccgtc ggttgcttcg cggttttctc cctgtcgatg ctcgtgcggt atcaaatccc
     1561 gttccgtcac gaagtcgaag aatgggtccg gcacaacgtc ggcgagcgat gggcgcgggc
     1621 gctgggctgg ttcgttctct gcctgccgct cgccacgtgg gccggggcgg gctgggtcgc
     1681 gctgtactgg gtgctgatca cgattcgctt catgcgtccg gcggagcggc tgacggcatt
     1741 cctgttgttg atcgtgaccg cgctcgcgct cccgctgtac cgcaccgccg tcggaaccta
     1801 tggagtgacg gccgaccccg tcgtgcggac gacgctccaa tcggccgaag gcgagtacga
     1861 tcccgacacg atcctctcgc tgcggaagct ggtacaggcc cacccgaacg atccggtcta
     1921 tcgcttcttg ctggccggcc tgtacaagaa cggacgctac tacgaagacg cattcgccga
     1981 gtacaaggag gcgctggcgc tcgacccggc gctcgagcag gcccacatca acatcggcaa
     2041 catctttttc gccacgggcc agtatgcgga agcgatcgcc agctacaacg aggcgatcgc
     2101 catcgatccg cggtcggtgc tcgcctactt caatcggcac ttggcccagt ccgagtcgtt
     2161 ccgcttccgg gaagccgagg cgacgatcga ggaggcccgg gcgatcgacg ccgacgcact
     2221 gagcgaactg ttcgcgacgg ccgacgtcga cggagaacga cgcaccgtcg ccgaggccag
     2281 cctcggcatg gggtcggtct ggcaagccgc cctcggtggg ggcgcagacg gatccggcgt
     2341 gaccaacccc gcggcgctcg agctcgcggc ggtcgcgcga cagttcgcca atccgatcag
     2401 tgcggcggcg gtcacggtcg cgttggcttg catgctgtta ctgatggcgg gtcgggcggg
     2461 gacaccggcg cgtcgctgca tccgctgtgg acggccgttc tgccaccgct gcaacacacg
     2521 gcgagactcg cacgagtact gcagtcaatg cgtccacctg ttcgtgctcg gcgatggcct
     2581 cgccgccgag accaagacgc gcaagctgta cgaggtcggt cgtcacgaac gacaatcgcg
     2641 cggggcgcgc agcgcggttt cgctggtgct gccgggcgcc gcacagctgc tgcgggggca
     2701 agcggcccgc ggcgtcctgc tggtcggcgc gtggctcgcg gcctggatcg cctggcgccc
     2761 gcagctgctc gagccgctcg accgcttggc cggggtcgac gtccggatcg acttgatgca
     2821 gcagccgagc gtgccggcgg gctttcagcc tcagccgctg gccctgctgg ccctcgtctc
     2881 gctggtcgtc gtctggctgt cgggcaacgc ctggcgttgg cgcaagcggg agggctgagg
     2941 cgggatggcc ctccagggaa cgctgaagga cttcggcctg ggagacattt tccagctgat
     3001 cggcatccag cggaagaccg ggatcctggc gctcgacaac ggcgacgaca gcgtcacggt
     3061 caagtttctc gaaggacaag tcgtcggcgc cgatacgaag cggcggagcg tcgaggagct
     3121 tctcggctcg gtgctggttc gcaccggtcg gatcagccaa tcgcagctcc aggacgcgct
     3181 gaagcggcag aaacgcacgt tgcaacggct gggctacgtc ctggtgcaga gcggtttgat
     3241 ctccgaagac gacctgatcg aagcactgcg cgtccagtcg ctgcagatca tctatcgcct
     3301 gttccgctgg cgagacggat cctacagctt tcgcggggtc gaagacctcg agtacgacga
     3361 gaagcatttt atcccgatca gcgccgagac gatcctgatg gaaggcgcgc ggatgatcga
     3421 cgagtggccg atcatcgagc ggcggatcaa atcggaccgc atcgtcctcc gggcgaccga
     3481 tgccgccgcc tcactgggac tgaccgtcga ggcgatcgtc gaccgggaca tcgggatcga
     3541 cccgaaccac ggtgccgaag gcggcgtccg cctctcgcgc gaggagcgcg aggtgctcgg
     3601 gctggtcgac ggacgccgca cggtcgagga gatcaacggc ttctccaccc tgggtgagtt
     3661 cgacacctat cgagtcttgt ccgagttgat gacgcgcaac ctgatcgagg aggtcaagcg
     3721 ggtcccggtg agaccggtcg gcggcataca cgtccggcgg ttgggagatc ggctgtttcg
     3781 ctggaccctg gacctgatcg tgctgggcgt ggtcgtcgcc ggggtcgcca gcctccgcgc
     3841 caatccgtgg gtgccgtgga ggatcgattc gctacgagcg acgaccgagc agttgcgcct
     3901 gcacgcctcc caggcccggc tcgagcacct ggagagcgcg attcaggttt actatctcga
     3961 tcgcggcgtc tttccgcagt cgctcgagct gttggcagcc aaccgctacg tgcggccggg
     4021 ggagacccgg gatccgtggg gtcgaccgta cggcttccgt ctgtcggcga gcggctacca
     4081 gttgtacggg tgggacggcg ccggcgaact acgaccgaac ttgacgctcg cacgtcgatt
     4141 caccgcgatc cagcgtatga tgagtggcct cgcggaggct gagccttcgg cgaacgagta
     4201 gtctaagttc tttgttgcga agaggatccc tcggtcggcg gggcggcggc cccccggccc
     4261 ggaccgtcct tgacagccga aacccggggt cttaacaagg cgggtgccaa acaaccgcga
     4321 gtctgccatt cgagacgcgt ccgtcccggg gagtagcaaa catgggcaag atcatcggaa
     4381 tcgacttggg tacgaccaac tcggtcgtcg ccgtgatgga ggggggcaag cccacgatca
     4441 tcaccaatcc ggaaggaagc cggctgaccc cgtccgtcgt tgccatcaac gacaagggcg
     4501 aacggctcgt cggtcaggta gcgaaacggc aggcgatcac caacccgacg aacacggtct
     4561 actcgatcaa gcggttcatg ggtcgcaagt tcagcgaggt ctcgcacgag gcccgggaag
     4621 ttgcgtacga cgtcctggcc gccgacaacg gcgacgtgcg cgtcaagatc cgcgacaaga
     4681 tgtacgcccc gcctgagatt tcggcgatgg tcctgcgcaa gctgaaggag gcggcggagg
     4741 ctcacctcgg cgagcgggtc gacgccgcgg tgatcaccgt gccggcatac ttcaacgatg
     4801 cccagcggca ggcgaccaag gacgccggtc agatcgccgg cttgaaggtc gagcggatcg
     4861 tcaacgagcc gaccgcggcc gcactggcct atggcctcga aaagaaaacg gacgagacga
     4921 tcgcggtctt cgacttcggg ggcggcacgt tcgacatctc gatcctcgaa gtcggcgaag
     4981 gtgtcgtcga ggtgaaggcg accaacggtg acacgcacct cggcggggac gacctcgatc
     5041 aacgcttgat cgagtggatc gtcgccgagt tcaagaaaga ccagggaatc gatctgtccc
     5101 aggacaagat ggcgttgcaa cggcttaaag aggccgccga aaaggccaag tgcgaattgt
     5161 ctacgaccag cgagaccgag atcaacctcc cgttcatcac cgccgatgcc aatgggccga
     5221 agcatctcca gctgaagctc aaccgggcca agttcgagca actcgtggga gacctgctcg
     5281 acaagacgct cgagccgtgt cgtcgcgcga tgagcgacgc cagcctcgag ccgtcggaca
     5341 tcgacgaggt cgtgctagtc ggcggttcga cccgcattcc gcgggtgcag cagatggtcc
     5401 aggagctgtt ccagcgtgag ccacacaagg gggtcaaccc cgacgaggtc gtcgccgtcg
     5461 gcgctgccgt gcaggccggc gtgctcggcg gagacgtgaa ggacatcctg ctgctcgacg
     5521 tgacgccgct gactctgggc atcgaaacgt tgggcggcgt cttcaccaag ctgatccagc
     5581 ggaacacgac gatcccgacg cgcaagagcg agatcttctc gaccgccgcg gacggccaga
     5641 ccagcgtcga agtccacgtc ctccagggcg agcgggaaat ggccgcttac aacaaaacgc
     5701 tcggcaagtt ccacctgacc gggatcccgc cggctccgcg cggcgtgccc cagatcgagg
     5761 tcaccttcga catcgacgcc aacggcatcg tcaacgtgtc ggccaaggac ctcgggaccg
     5821 gcgcggagca gaagatcacg atcacggctt cctccggtct gtcgggcgac caagtcgaga
     5881 agttggtcaa agacgcgacg gtccacgccg ccgaggaccg ggaggcccgc gaagcggtcg
     5941 acgcgcgcaa caagtgcgac caactggtgt accaggtcga gaagctgctc aaggacaacg
     6001 atgcgaagct gtccgagcgg gaccaggccc cggtgcgcga cgcgctggcg actgcgaaga
     6061 aggccctgga ggccggcaac gtcggtgaga tccgggcggc gacggagcga ctggaaaaag
     6121 ccagccacca gctggccgag gcgatctacc gatcccgggc cgatgccggt gtctcgaccg
     6181 ccgacgaggc cacggccggg gcgcgcggtt cgagcggatc cggcggcaac ggccacggcg
     6241 aggtgatcga cgccgaggtc gtcgacagcg attcatcggg tcgttgaccc acaccgcgta
     6301 ggcggcggag gcaggcgatg aagaaggggg cggactggaa cccgctgagt gagctgttgg
     6361 gcgtacaaaa gcggatgaac cagctgttcg aatccgccct gactcgctcc gagatcgagg
     6421 cccatgacgg cttgggcgtc tggattcccg tgtgcgacgt ctacgagaac gctggcggca
     6481 cgctggttgt cgggatcgag ctgccgggta tcgaccgcag gtcgatcgac gtgcggatcg
     6541 acgatgacga gctcgtcatc gagggttgcc gcgagatgga ccgcgagcgc gagggcgagc
     6601 attttcaccg cgtcgaacgc tcctacggca agttctcgcg tcgcttccgc cttccgtcga
     6661 agatcgaccg ggagtcggtt accgcggcgt acaaggacgg cgtgctgacc gtcacgctgg
     6721 cccgacgcga cggagccaag ccggggccga tccaggtgtc ggtctcctga gagattcacg
     6781 cggggcgatg acgtcttcgt cgtcgtcgat tcacggtcga aaccccttgc aaagcgcggc
     6841 tttgccacaa atattgcgtg ctatactgcc gccttgttga cggggttgga atggaaagag
     6901 aacgcgaaga aacccgcagc tccggttccg aatccctcaa gaagtacctc aaagagatct
     6961 cccggcttcc tcggatcacc gttcaggagg aacgcgacct cggcgctcgg atccaaaaag
     7021 gggacaccaa ggcgctgagg cggctggtcg aggcgaacct acgcttcgtc gtctcctacg
     7081 ccaagcgcta tcgcggctgc ggtctgtcgt ttctcgacct gatcaacgag ggcaacatcg
     7141 gcttgatcga ggcggccaag cgattcgatc cggacaaggg cgtcaagttc atcacctacg
     7201 cggtgtggtg ggtgcgccaa gcgatcatcc acgcgctctc cgaccagagc ggcgcattcc
     7261 gtctcccgca gaagcaggcc aacctgctct accggatcgg caaggcgcag gccaacctgc
     7321 ggatcgagct caagcgaacg ccatcgaccg aggagatcgc ccgtaagatg gaagtaacgg
     7381 tcgaggaggt gaacaacctg ctccaggtct ccgacgacaa catctcgttg tcggccgtca
     7441 tcgatgagga acacgacttc catctgtcgg acaagctcga gcaagagacg atcccgtcgg
     7501 cggatctcgt catgctgaag aactcgctcc gcgggctgtt gcgtaacgct ctgaccgagc
     7561 tggatcccaa ggaagaacag gtgctgcgcc tgcgcttcgg cctcgacggg accgacccca
     7621 agacgctgaa ggagatcggc gagatgttga gcctctcccg cgagaggatc cgtcagatcg
     7681 aagcccaggc gctcgaaaaa ctgaaccgct cgcacaagtg ccaacagctg cgggggtacc
     7741 tcaactgatc ggtacgagcc ggtcggtgct cgccggacgg agtgcatgga tggcggccga
     7801 atgtccgaac tgccacggga ccggcttcga gctccggtcg agccagaacg gcgtcatcac
     7861 ctccaagcgc tgtgcctgcg atctggctcg cttgggcgag cggttgctgc gtcgggcgcg
     7921 gattccgccg cgctacgaac actgcacgtt cgacgagttc cggatccgcc ccgacgacga
     7981 gagccacgca cacgccctgg ccacggcccg cgagtgggtc ggactgtggc cgacggtgac
     8041 aagcgggttg ttgttcctcg gtccgccggg aaccggtaag acccatctcg cggtggcgat
     8101 cgctcgcgaa ctggccggtg cgaagaacgc ggacgtcttg ttctacgagc agcgcgagct
     8161 gatgaaatcg ctgcaagcca cgttcgagtc gggctcgtcg cagcgtgagt cg
//
LOCUS       DASPGN010000498         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_911867, whole genome shotgun sequence.
ACCESSION   DASPGN010000498 DASPGN010000000
VERSION     DASPGN010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911867"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VD788_14470"
     CDS             <1..417
                     /locus_tag="VD788_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77514.1"
                     /translation="DLLTSYNVVEHLYDLNAFAGQTFRMLAPGGRAIHRVDFGPHGNW
                     LERANPLEWLTVPDVLWSLMGSMRATPNRRRFHEVHLALMRAGFVVDAVESEHFAEAD
                     LTAIRPHLPRRFRSMPDASLRVKTASYVCRRPEAPC"
     gene            complement(544..>2079)
                     /locus_tag="VD788_14475"
     CDS             complement(544..>2079)
                     /locus_tag="VD788_14475"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXV77515.1"
                     /translation="DLQRGLVDISGVGDASRFFFGADVRDLDLARSALLAGMIRSPGR
                     YNPRRNPAAALERRDLVLRLMHEHERIDDRQLGEALAQPLELTETGRAGAAERLPWVE
                     DWLEDEIAALSPEAVPSRAGYSVFTTFDPAIQRAAESALESGLGTLERRKGGDGGEPL
                     EGAVVVLRPADGSLLALVGGRNHGRSQFNRATQAHRSPGSTFKPFVFLAGLERASRDP
                     DFAFTAATVLDDSPLELRAGGKLWNPTNFDRTFRGSVTVRDALEQSINVPTVRAAVQV
                     GLPEVVRVAHDCGITSDLAPVPALALGAGEVTPLELATAYATLANGGWRVRAHGLVTV
                     LDRAGRLVERAAQPEQRVIEPELAYLVTNLLEGVVERGTARSAAALGFTGTAAGKTGS
                     SNELRDAWFVGYTPEVLALVWVGYDDNRPVGLAGGAAALPIWVDLMRRIGATDAPPFE
                     RPPGIVREKIDPTTGQLATRRCPDRREEIFVGHALPERCTLHDRGEPEGLWKRLFGRR
                     RPG"
BASE COUNT          298 a          775 c          709 g          297 t
ORIGIN      
        1 gatctgctca cgtcgtacaa cgtcgtcgag cacctctacg atctgaacgc cttcgccgga
       61 cagacctttc gcatgctcgc tccgggaggt cgcgcgatcc accgcgtcga cttcggcccg
      121 cacggcaact ggctcgagcg ggccaatccc ctcgagtggt tgaccgtgcc ggacgtcctg
      181 tggagcctga tggggtcgat gcgcgcgacg cccaatcgaa ggcggttcca cgaggtgcat
      241 ctcgcgctga tgcgcgccgg tttcgtcgtc gacgcggtgg agtcggagca cttcgcagaa
      301 gccgatctga cggcgatccg gccgcacctt ccgcggcgct tccgctcgat gcccgacgcg
      361 tcgctgaggg tcaagacggc gagctacgtc tgccggcgcc ccgaagctcc ctgttgaacg
      421 gggccggtcg tcatcgaggc cgaaccgtcc ccgcgcgagc gacggcgacg ttccgcgcga
      481 atcgagtcgt gtgcccgtct cccatccggg tcccggcggg cgcgatggcc atgaccccga
      541 ttctcatccg ggccgccgcc gtccgaacag tcgcttccac agcccctccg gctcgccgcg
      601 atcgtggagg gtacagcgct ccggcaacgc gtggcccacg aagatctcct cgcggcggtc
      661 ggggcatcgg cgggtggcga gctggccggt cgtcggatcg atcttctcgc gcacgatgcc
      721 ggggggacgc tcgaacggcg gggcgtccgt cgctccgatg cgccgcatca gatcgaccca
      781 gatcggaagc gcggcggcgc ctcccgccag accgaccggg cggttgtcgt cgtagccgac
      841 ccacacgagc gccagcacct cgggcgtgta cccgacgaac caggcgtccc gcagctcatt
      901 gctcgacccg gtcttccccg cggccgtgcc ggtgaagccc agcgcggcgg cgctccgcgc
      961 ggtgccgcgc tcgacgactc cctcgagcaa gttcgtcacg aggtaggcga gctccggttc
     1021 gatcacgcgc tgctccggct gcgccgctcg ctcgacgagc ctacccgcgc ggtcgagcac
     1081 cgtcacgagt ccgtgcgccc gcacgcgcca tccgccgttg gccagcgtgg cgtaggccgt
     1141 cgccagctcg agcggtgtga cctctccggc gcccagggcc agggccggaa ccggcgcgag
     1201 atcgctcgtg atgccgcaat cgtgcgcgac gcgcacgacc tccggcagcc ccacctgcac
     1261 cgccgcgcgc acggtcggca cgttgatcga ctgctcgagc gcgtcgcgca cggtcaccga
     1321 gccccggaag gtccgatcga agttggtcgg gttccacagc ttgccgccgg cgcggagctc
     1381 gagcggcgag tcgtcgagca cggtcgccgc ggtgaacgcg aaatccggat ccctcgacgc
     1441 acgctcgagc ccggcgagga acacgaatgg cttgaacgtc gagccgggag accggtgtgc
     1501 ctgggtcgct cggttgaact ggctccgtcc gtggttccgc ccgccgacga gcgcgagcag
     1561 cgacccgtcc gccggacgca gcaccaccac cgcgccctcg agcggctcgc cgccatcgcc
     1621 gcccttgcgc cgctcgagcg tacccaagcc cgactcgagc gcagactcgg cggcacgctg
     1681 gatcgccggg tcgaacgtgg tgaagacgga gtagcccgcg cgtgacggca cggcctcggg
     1741 agacagggcc gcgatctcgt cctcgagcca gtcctcgacc cacggtagcc gctcagccgc
     1801 gccggcccgc ccggtctcgg tcagctcgag cggttgcgcc agcgcttcgc cgagctgccg
     1861 atcatcgatc cgctcgtgtt cgtgcatcag ccgaagcacc aagtcgcgcc gctcgagagc
     1921 cgccgcggga ttccgccgcg ggttgtaccg gcccgggctg cggatcatcc cggccagcag
     1981 tgcggaccgt gccaggtcga gatcgcgcac gtcggcgccg aagaagaaac gcgaggcgtc
     2041 gccgacgccc gagatgtcca caaggccgcg ctgcaggtc
//
LOCUS       DASPGN010000499         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_328122, whole genome shotgun sequence.
ACCESSION   DASPGN010000499 DASPGN010000000
VERSION     DASPGN010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328122"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VD788_14480"
     CDS             complement(<1..561)
                     /locus_tag="VD788_14480"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV77516.1"
                     /translation="MMEIIRSGGPVMYPLLICSLLVVAIIVERAINLRASRVLTPTTV
                     ERIHGLVEGGRTDRAIEICRQSPGMFSSIMLPALELADRGEAAAKEAVEDAGRHETAR
                     LTRYLTALGTIAAVAPLLGLLGTVTGMIEVFRTIARSGVGQAAELSGGISQALVTTAF
                     GLLIAIPALVGYNYFQSKVESMVTELE"
     gene            complement(623..1894)
                     /locus_tag="VD788_14485"
     CDS             complement(623..1894)
                     /locus_tag="VD788_14485"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXV77517.1"
                     /translation="MSITFRDFGLTGEIIGALDAAGYTSPTPIQRQAIPILLEGGDLI
                     GVAETGTGKTLAFLLPIFERLERDRQDAQALIVCPTRELALQVAGESARFGAVSGVRT
                     VVAYGGTSSGEQKKQLAAGCDIVVGTPGRLLDFLNSAWLSVRRVRTVVLDEADRMLDM
                     GFIADVDAILRRAPMSRQTLLFSATFPEEIRRLSQRYMFHPRIVRMHEGTRVTKNVEH
                     VVYPVEASKKERLLFELLRRERPEKFLVFTATREATSRLALALRRQGHEVISLSSLLS
                     QANRERALGAFRRGECSALVATDVAARGLDITDIDLVVNFDLPLQPQDYVHRVGRTGR
                     AERAGRAVTLVTPEDEPRAARIEKLLRHDLVRLQLEGFTRPPGAPAGGRRDAGSGRSR
                     SRGSGRGGRGGGSRRRPGPASGGRRSNGGGE"
     gene            complement(1910..>2853)
                     /locus_tag="VD788_14490"
     CDS             complement(1910..>2853)
                     /locus_tag="VD788_14490"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77518.1"
                     /translation="TVIRDIEPIVEIAQRAGIAIEACLFIGSSPIRQYAEGWDEEFLL
                     RQSAEAIEFAVGHELPVMYVTEDTSRARPETVRRLYGEAVRLGAKRVCVTDTVGHATP
                     DGVVRLVRFIRQAIDDAGGSEVGIDWHGHRDRGMGLANCIAAYRAGATRVHGCGLGIG
                     ERCGNAEIDLLLVNLKLLGWIDRDLTRLADYCRAVSAAVGVPIASNYPVVGSDAFETA
                     TGVHAAAVIKAYRKGDEWLADRIYSGVAAGEFGFKQKIRIGPMSGRSNVSYWLADHGI
                     EPTDERVDRIFEAAKRSDRLLEADEILGLIGQGNRPV"
BASE COUNT          457 a         1032 c          928 g          436 t
ORIGIN      
        1 ttcgagctcg gtcaccatcg attcgacctt ggattgaaag tagttgtagc cgacgagtgc
       61 cggaatcgcg atgagcagcc cgaatgcggt cgtcaccagc gcctgggaga tcccacccga
      121 cagttccgcg gcctggccca cgcccgagcg ggcgatcgtc cggaacacct cgatcatacc
      181 ggtcacggtg ccgagcaggc ccaacagcgg tgcgacggcg gcgatcgtcc cgagcgccgt
      241 cagatagcgc gtcaggcggg ccgtctcgtg gcgtcccgcg tcctcgacgg cctccttggc
      301 ggccgcctcg ccgcggtcgg cgagctcgag cgccggcagc atgatgctcg agaacatgcc
      361 ggggctttgg cggcagatct cgatcgcgcg gtcggtccgt ccgccttcca ccagcccgtg
      421 gatacgctcg accgtcgtcg gggtcaacac ccgcgaggca cgcaggttga tcgcgcgctc
      481 gacaatgatg gcgacgacca gcagcgagca gatcaacagc ggatacatca ccggtccgcc
      541 cgaccggatg atctccatca tcgccatggc agccatggta gagggccgat ttcggccccg
      601 caaacggtcg gctcaccctc ggtcattcgc cgcccccgtt cgatcgccgg ccaccgctcg
      661 ccgggcccgg gcgtcgacgc gaaccgcccc cgcgaccccc gcgaccactc ccgcggctgc
      721 gcgaacgacc cgaccccgcg tcccgccgac cgcccgccgg cgcccccggc ggacgcgtga
      781 acccctcgag ctgaagccgc accaggtcgt gccgcaacag cttctcgatc cgcgccgcgc
      841 gcggctcgtc ctccggcgtc accagggtca ccgcccgacc ggcgcgctcg gcccgaccgg
      901 tgcgtccgac gcggtgaaca tagtcctgcg gttgaagcgg aaggtcgaag ttgacgacga
      961 gatcgatgtc ggtgatgtcg agtccgcggg cggcgacgtc ggtcgccacc agcgcgctgc
     1021 actcgccgcg acgaaatgcc cccagcgcac gctcgcggtt cgcctgcgac agcaggctcg
     1081 acaacgagat cacctcgtgt ccctgccgcc gcagggcgag ggccagccgc gacgtcgcct
     1141 cgcgcgtcgc ggtgaagacc aggaacttct ccgggcgctc ccgtcggagc agttcgaaca
     1201 acagccgctc cttcttgctg gcctcgaccg ggtagacgac gtgctccacg ttcttggtca
     1261 cccgcgtgcc ttcgtgcatg cggacgatcc tggggtggaa catgtatcgc tgtgacagtc
     1321 tccggatctc ctcgggaaag gtcgccgaga acagcagcgt ctgccggctc atcggcgccc
     1381 gtcgcagaat cgcgtcgacg tcggcgatga agcccatgtc gagcatgcga tcggcctcgt
     1441 ccaacacgac ggtccgcacc cgccgcacgg acagccatgc cgagttgaga aaatcaagca
     1501 gccttccggg ggttccgacg acgatgtcgc agcctgcggc gagctgcttt ttctgctcgc
     1561 cgctcgacgt tccgccgtac gcgacgacgg tgcgcacgcc gctcaccgca ccgaagcgcg
     1621 cggactcgcc ggcgacctgc agcgccagct cgcgtgtggg acagacgatc agcgcctgcg
     1681 cgtcctgccg gtcacgctcg agccgctcga agatcggcag caggaaggcg agggtcttgc
     1741 cggtcccggt ctcggccacg ccgatcagat ctccgccctc gagcaggatc gggatcgcct
     1801 ggcgctggat cggcgtgggg ctggtgtagc ccgccgcgtc gagagcacca atgatctcgc
     1861 cggtgagacc gaaatccctg aatgtgatcg acatcgggcg ggaactatat cagacgggtc
     1921 ggttcccctg cccgatcagg cccaggatct cgtcggcctc gagcagccgg tccgagcgtt
     1981 tggcggcctc gaagatccgg tcgactcgct cgtcggtcgg ctcgatgccg tggtcggcga
     2041 gccagtagga gacgttcgag cgcccgctca tcggtccgat gcggatcttc tgcttgaagc
     2101 cgaactcacc ggccgcgacg cccgagtaga tgcggtcggc caaccactcg tcacctttgc
     2161 ggtaggcctt gatcaccgca gccgcatgca cgccggtcgc cgtctcgaag gcgtcgctgc
     2221 cgacgaccgg atagttgctc gcgatcggca ccccgaccgc cgccgacacc gcgcggcagt
     2281 agtcggccag ccgggtcaga tcgcggtcga tccaaccgag cagcttgagg ttgaccagca
     2341 gcaggtcgat ctccgcgttt ccacagcgct cgccgatccc gaggccgcag ccgtggacgc
     2401 gggtagcgcc ggctcgatag gcggcgatgc agttcgccag tcccatgccg cggtcacggt
     2461 ggccgtgcca gtcgatgccg acctcactgc cgcccgcgtc gtcgatcgct tggcggatga
     2521 aacggaccag gcggacgacg ccatcggggg tcgcgtggcc gacggtgtcg gtcacgcaga
     2581 cgcgcttcgc gcccaggcgc accgcttcgc cgtacagccg gcgcaccgtc tccggacgcg
     2641 cacggcttgt atcctcggtg acgtacatca ccggcagctc gtgcccgacc gcgaactcga
     2701 tcgcttcggc cgactggcgc agcaggaact cctcgtccca accctcggcg tactggcgaa
     2761 tcggcgagga accgatgaac agacaagcct cgatcgcgat gccggcccgc tgcgcgatct
     2821 cgacgatcgg ctcgatgtca cgaatcacgg tgc
//
LOCUS       DASPGN010000500         8248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_912353, whole genome shotgun sequence.
ACCESSION   DASPGN010000500 DASPGN010000000
VERSION     DASPGN010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912353"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /gene="rny"
                     /locus_tag="VD788_14495"
     CDS             complement(<1..1510)
                     /gene="rny"
                     /locus_tag="VD788_14495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HXV77519.1"
                     /translation="MDGTGPLVLAALASVIAGGVAGWLLNNRFGAKSLEAMRKRTDEA
                     LRAARREAEKSRRKLLLEAREQILQQRNKTERDLRSRRGQLNRRERDLQAGLKNLEDQ
                     QAEGARERERLTETARKIEAELDEASRAREESAKLIELQTARLSELAEMTRDEAQRQL
                     LANLKAETRLEAANLIKEIKDEAQRNADAEATKITALAIERMASDLSAERTVSLFELP
                     AGTELMGRIIGQEGKNIKAFELATGIQLVLDEAARTVTLSGYHPVKREIARRVLETLV
                     SDGNIHPRRIEELTRRNRRRLEEEMKRAGGETLKELNIKGVHPELAKLLGRLKFRSSY
                     AQNVLMHSKEVAYLTGMLAAELRLDEGLARRAGLLHDIGKAIDYEREGTHPEIGAEVA
                     SKFGEPEVVVNAIASHHEDCEMTSPISVLVSAADSLSGARPGARRKTVAEYIKRIDKL
                     EELANSMRGVEQSYAIQAGREIRVIARSREVDDAQADLLATDLAARIQSEMDY"
     gene            complement(1593..3407)
                     /locus_tag="VD788_14500"
     CDS             complement(1593..3407)
                     /locus_tag="VD788_14500"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXV77520.1"
                     /translation="MTQQSRGDLRNLAIIAHVDHGKTTLVDAMLWQSGIFGETGDVAD
                     RVTDSIDLEREKGIPVMPPIVSVAYRGIRIHIVDVPGHADFGRGVDRALQLVDGLLLV
                     VDASEGPLPQTRFALRRALEAGLAPIVVVNKIDLPGARPDRVVDEVRELFVDLDARPE
                     QLAFPALFCNARRGQCRLTADGEDHPLLPLFEEIVGRVPAPRFAAEQPVQFRVSSIDY
                     DDYLGRLAVGRVVNGSLELGGEVMQCHADGTRGAARIAGLFAFDGLRRIEAEQAGPGE
                     IVCVSGLEGVAIGDTLTDRERPVVLPPLPADDPTLSMSIAVNDSPMAGVEGTLTSADD
                     LRERLFRELLTNPLIHVEETASTEVFTLSGCSELQLAILIEMMRREGYELSVGKPAVL
                     TRKVDGAVEEPIELLVVDCPEEYVAVVTEKIGPRRGHLARMVNHGTGRVRLEFRLPTR
                     GLFGFRAEFLSDTRGAGILNHRIESYQPWQGEIAGRSTGALIADRPGRATAFAIEHLQ
                     PRGTIFVAPGDEVYEGMIVGENSRANDLIVNITKEKRPSPGPTPARRPAVHLIPPRSM
                     SLEQALEFLRDDDLVEVTPRSVRLRKKILQAAVRQRNT"
     gene            3605..4438
                     /locus_tag="VD788_14505"
     CDS             3605..4438
                     /locus_tag="VD788_14505"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HXV77521.1"
                     /translation="MENEEPRDPDLEPVEAIYDALDYGDAETALMLARRSLAEAGESG
                     QDPVLHFLAGVALLELDRPADAVVALELAVRLDPDDAEFHSRLAEALFSCCRFDQAEH
                     EARRAIAADSRLPHGYWMIGLALERSGDYHAADNAVQKAADLSPDEFYVPVRMSREEF
                     DQQLGTAIASLPERFRAHLDDVTVAVEDLPSDELLLAEDPPLDPELLGLFVGAPCNER
                     SVLSPGGELPAMIYLFQRNIERVSIDNEELREEIAITLRHELGHYLGLDEDEIARAGH
                     A"
     gene            4530..5954
                     /locus_tag="VD788_14510"
     CDS             4530..5954
                     /locus_tag="VD788_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77522.1"
                     /translation="MSFERKWTPLVGAILMLADPAGAAQAGRPLSAAEARQEVRLVVD
                     NLMSFHPDPFHETTRQAFFAEVATLLAGEGPVTAPEQFFRLARLLSPISDTHTQLHFT
                     DDTPGFDTTYPLRFRLFPDGLYIIAGNEDYRDAIGKKVVTIAGHQATEVVDRFAHYSF
                     ADHWPRKRVFAEMLFYHPATYDFFDLRTARGTTELVVESSTGERATVELAATWDKRAS
                     QFGWDMLNPFIPDGLLTVHDVLGTEPPYYQRRIDDNVWFGFPAGNDRLMYLQINRQLE
                     PEDGPESIDIILDWARTLWESAAEVLVIDLRNDPGGNIFLGDAISGILPELYVNHPTL
                     KAVAVLFGTDTVSAGTILVAQLERTVQPVLIGDPSGSAPNMFLHAEKIVLPHSNIEFE
                     VSREEYVTTRDLDTRRYIVPDVPMSLSFADFANGRDPLLDFAATIDDALAQKIYGAVG
                     LDAWRRDSQRPALPSHASRTAAAR"
     gene            complement(5987..6532)
                     /locus_tag="VD788_14515"
     CDS             complement(5987..6532)
                     /locus_tag="VD788_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77523.1"
                     /translation="MRILTAIVVPLLLAGAACHDGSGTTGGGVFDATGTWSVEVVTTG
                     PPPPGCTIVDGSAVGLPTIGGLVELAASGHPIVVDQANSRITMRPIEYVDGAVTHRWA
                     LDGTVGAPGVEARLTHVVKLQHSSPALAAIGVERLSGISLSPRRLLLTGDGVAFVPDP
                     AAAELEVCSAAPTATYRVSAL"
     gene            complement(6624..7034)
                     /locus_tag="VD788_14520"
     CDS             complement(6624..7034)
                     /locus_tag="VD788_14520"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HXV77524.1"
                     /translation="MILLARRPIDVAELLQAVGGPGNGAVALFVGTVREQNQGRRVLH
                     LEYHAYDEMALREMSALRQAALERFALGEVAIAHRIGKLEVGEASVAIAVSAPHRAAS
                     FDACRWIIDRLKQRVPIWKKEFFEGGEVWIEGPN"
     gene            complement(7037..7282)
                     /locus_tag="VD788_14525"
     CDS             complement(7037..7282)
                     /locus_tag="VD788_14525"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXV77525.1"
                     /translation="MKLDVRYFASLAETTGTPRETIEIDAGSDVGALWELLSVRHAAL
                     AGLHYRPLVACDMRYCDWSESLDGVREVAFLPPISGG"
     gene            complement(7279..>8248)
                     /locus_tag="VD788_14530"
     CDS             complement(7279..>8248)
                     /locus_tag="VD788_14530"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HXV77526.1"
                     /translation="FVLVTHINPDGDAVGSEWALASYLLDQGKRVRIVNQDRAPESMR
                     FVGFDRVPIEIYDAATHDSLIASAGRILLVDNSAPDRLGRMERVMRASAARTLCIDHH
                     PTRDAPWADSIVDVHYCATTAMIYDLARGVGWAPDEVAALAIYVGIATDTGFFRFNST
                     NPRAHRIAAELIDLGVDPARVYREIYERNSVSYVRLLGHALTDLRMDAGGAIASVRIA
                     RELIERLDAEEVDVTEITTSLLALDGVRVAVLYRELADGRVKVSLRSKGTLDVHRLAI
                     EFGGGGHRNASGIVMDGGLDRSMRIVTGRVAAMVAATDQGPARAER"
BASE COUNT         1297 a         2913 c         2728 g         1310 t
ORIGIN      
        1 ggtagtccat ctcgctctgg atgcgcgcgg cgaggtcggt cgccagtaga tcggcctgcg
       61 cgtcgtcgac ctctcgcgag cgcgcgatca cgcggatctc gcgaccggct tgaatggcgt
      121 aggactgctc gacgccccgc atcgagttcg ccagctcttc gagcttgtcg atgcgtttga
      181 tgtactccgc gacggtcttg cgcctggcac ccggccgcgc gccggacagc gaatcggcgg
      241 ccgacacgag caccgatatc ggcgaagtca tttcgcagtc ttcgtggtgc gacgcgatcg
      301 cgttgaccac gacctcgggc tcgccgaact tgctcgcgac ctcggcgccg atctcgggat
      361 gcgtcccctc gcgctcgtag tcgatcgcct tgccgatgtc gtgcaacagg cccgcccggc
      421 gtgcgaggcc ctcgtcgagc cgcagctccg cggccaacat gccggtcagg taggccactt
      481 ccttggagtg catcaacacg ttctgcgcgt aggacgagcg gaacttcaac cggccgagca
      541 gcttggccag ctcggggtgc acgcccttga tgttcagctc cttcaacgtc tcgccgcccg
      601 cgcgtttcat ttcctcttcc agacggcgac ggttgcgccg cgtcaactcc tcgatccggc
      661 gcgggtggat gttgccgtcg ctgacgagtg tctcgagcac gcgccgagcg atctcgcgct
      721 tgaccgggtg gtaacccgac aaggtcaccg ttcgcgccgc ttcgtccagt acgagctgaa
      781 tgccggtcgc gagctcgaac gccttgatgt tcttgccttc ctgcccgatg atgcggccca
      841 tcaactcggt gccggccggc agctcgaaca ggctgaccgt gcgctcggcg ctcagatccg
      901 acgccatccg ctcgatcgcc aacgcggtga tcttggtcgc ctcggcgtcg gcgttgcgct
      961 gcgcctcgtc tttgatctcc ttgatcaggt tggccgcttc caagcgcgtc tcggccttga
     1021 ggttggccag cagctggcgc tgcgcctcgt cacgggtcat ctcggccagc tcgctgagcc
     1081 gcgcggtctg cagctcgatc agcttggccg attcctcccg cgctcggctg gcctcgtcga
     1141 gctcggcctc gatcttgcgc gcggtttcgg tcagccgctc ccgctcgcgc gcgccctcgg
     1201 cctgctggtc ctccaggttc ttcaaaccgg cctgcagatc gcgctcgcgc cggttcaact
     1261 ggccccgacg ggatcgcagg tcgcgctcgg tcttgttgcg ctgctggagg atctgctccc
     1321 gcgcctcgag cagcagcttc ctgcggctct tttccgcttc gcggcgcgcg gcgcgcagcg
     1381 cctcgtcggt ccgcttcctc atcgcctcga ggctcttggc gccgaagcgg ttgttcaaca
     1441 gccagccggc cacgccaccg gcgatcacgc tcgcgagcgc ggccagcacc agcgggcccg
     1501 tcccgtccat ccgcggatct cctcgctcga tgccactcgt ccgcggtcat tatcgcccgc
     1561 cggcgagcag cgtcaagcaa ctcgttgaaa aatcaagtgt tgcgctgtcg gaccgctgcc
     1621 tgcaggatct ttttgcgcag ccggacgctg cgcggggtga cctcgaccag atcgtcgtcg
     1681 cgcaggaact cgagcgcctg ctcgagactc atcgaccgcg gcgggatcag gtgcaccgcg
     1741 ggccggcgcg cgggcgtcgg accgggcgac gggcgctttt ctttggtgat gttgacgatc
     1801 aggtcgttcg cgcgcgagtt ctcgccgacg atcatcccct cgtagacctc gtcgcccggc
     1861 gcgacgaaga tcgtcccacg cggctggagg tgctcgatcg cgaacgccgt ggcgcggccc
     1921 gggcgatcgg cgatcagcgc cccggtgctg cgaccggcga tctctccctg ccacggctga
     1981 taggactcga tccggtggtt gaggatgccc gcaccacgcg tgtcgctgag gaactcggcg
     2041 cggaagccga acagaccgcg tgtcggcagg cgaaactcga gccgcacccg tccggtgccg
     2101 tggttgacca tcctggccag gtgaccgcga cgcggaccga tcttctcggt gacgacggcg
     2161 acgtactcct cggggcagtc gacgacgagc aactcgatcg gctcctcgac ggcgccgtcg
     2221 accttgcgcg tcagcaccgc gggcttgccg accgacagct cgtaaccctc acggcgcatc
     2281 atctcgatca ggatcgcgag ctgcagctcg ctgcaacccg acagggtgaa cacctccgtc
     2341 gaggccgtct cctcgacgtg gatcaagggg ttggtcaaca gctcgcgaaa cagacgttcg
     2401 cgcagatcgt cggcgctggt cagcgtgccc tcgacgcccg ccatcggcga gtcgttgacc
     2461 gcgatcgaca tcgacagagt cggatcgtcg gccgggagcg gggggagcac caccgggcgc
     2521 tcgcgatcgg tcagcgtgtc gccgatcgcg acgccctcga gcccggagac acagacgatt
     2581 tcgccgggac cggcctgttc ggcctcgatc cgccgcagac cgtcgaaggc gaacaacccc
     2641 gcgattcgcg cggccccacg ggttccgtcg gcgtggcatt gcattacctc gccgccgagc
     2701 tcgagcgagc cgttgacgac ccggccgacg gccagtcgcc cgagataatc gtcgtagtcg
     2761 atcgagctga cccggaactg aaccggctgt tcggcggcaa agcgcggggc gggaacgcga
     2821 ccgacgatct cctcgaacag cgggagcaac gggtggtcct cgccgtcggc ggtcagacgg
     2881 cactggccgc gccgcgcgtt gcagaacagc gccgggaagg ccagctgctc gggtcgggcg
     2941 tcgaggtcga cgaacaactc gcgcacctcg tcgacgacgc ggtcgggacg cgctccgggc
     3001 aagtcgatct tgttcacgac cacgatcggc gccaaccccg cctcgagcgc gcggcgcagc
     3061 gcgaagcgtg tttgcggcaa cggtccctcg ctcgcgtcga cgaccagcag cagaccgtcg
     3121 accagttgga gcgcccggtc gacgcctcga ccgaagtcgg cgtggccggg gacgtcgacg
     3181 atgtggatcc gtatgccgcg gtaggcgacc gacacgatcg gcggcatgac ggggatgccc
     3241 ttctcgcgct cgagatcgat cgagtcggtc acccggtcgg cgacgtcgcc ggtctcgccg
     3301 aagatcccgc tctgccacag catcgcgtcg accagtgtcg tcttcccgtg gtcgacgtgg
     3361 gcgatgatcg ccaggttgcg gagatcaccg cgggattgct gcgtcatcgc tcgctccgtc
     3421 ggaaacgaaa aaagccggga atcggcgggc cgccggccgg actcccgacc gatcaaccgc
     3481 aatcccggcc tccgccgaac gataacacac aacgcggtgc agggctgcca acttgccggg
     3541 gagaccgtgc gatgcgacaa tccggcgacg atccggccat cgccggtgcg ggaagagacg
     3601 caacatggaa aacgaagagc cgcgcgatcc ggatctcgag ccggtcgagg cgatctacga
     3661 cgcgctcgac tacggcgacg ccgagaccgc gctgatgctc gcgcggcggt cgctggccga
     3721 ggccggcgag agcggtcagg atccggtgct gcactttctc gcgggggtgg cgctgctcga
     3781 gctcgaccgg ccggccgacg cggtcgtcgc actcgagctt gcggtgcggc tcgatccgga
     3841 cgatgccgag ttccattccc gactggccga ggcgctgttc tcctgttgtc gcttcgacca
     3901 agccgagcac gaggcccgcc gcgcgatcgc ggcggactcc cgcttgccgc acggatactg
     3961 gatgatcggc ctggcgctcg aacggtcggg cgactaccac gcagccgaca acgccgtgca
     4021 gaaggccgcc gacctgtcgc ccgacgagtt ctacgtcccg gtccgcatga gccgcgagga
     4081 gttcgaccag cagctcggaa cggcgatcgc ctcgctcccc gagcggttcc gcgcgcacct
     4141 cgacgacgtg accgtcgcgg tcgaggacct gccgagcgac gagctgctgc ttgccgaaga
     4201 tccgccgctc gaccccgagc tgctcggctt gttcgtcggc gcgccgtgca acgaacggag
     4261 cgtgctctcg cccgggggcg agctgccggc gatgatctac ctgttccagc gcaacatcga
     4321 gcgcgtatcg atcgacaacg aagagctgcg cgaagagatc gcgatcaccc tgcgtcacga
     4381 gctgggtcat tacctcggcc tcgacgagga cgagatcgcg cgcgccgggc acgcctagcg
     4441 ctcgacgccg gccttcccaa ccatcggcgc cccggcggcg tctggtgtcg agcgggccgt
     4501 caccgggtcg gcccggtcgg gagggaccga tgtcgttcga acgcaaatgg accccgctcg
     4561 tcggggcgat cctgatgctg gccgatccgg cgggggcggc gcaggccggg cggccgctgt
     4621 ccgcggccga ggctcggcag gaggtccgac tcgtcgtcga caacctgatg agcttccatc
     4681 ccgatccgtt tcacgagacc acgcgccagg cgttctttgc cgaggtcgcg acgctgctgg
     4741 cgggcgaggg tccggtcacg gcgcccgagc agtttttccg gctcgcgcgc ctactgtcgc
     4801 cgatctcgga cacgcacacc cagctccatt tcaccgacga cacgccggga ttcgacacga
     4861 cctatccgct ccgattccgc ctgttccccg acggcctgta catcatcgcc ggcaacgaag
     4921 actaccgcga cgcgatcggc aagaaggtcg tgacgatcgc cggacaccag gcgacggagg
     4981 tcgtcgaccg tttcgcgcat tactcgttcg ccgaccactg gccgcggaaa cgtgtgttcg
     5041 ccgagatgct gttctaccat ccggcgacgt acgatttttt cgacctccgc acggcacgcg
     5101 ggacgaccga gctcgtcgtc gagagctcga ccggcgagcg cgcgaccgtc gaactggcgg
     5161 cgacctggga caaacgcgcc tcccagttcg gctgggacat gctgaatccg ttcatcccgg
     5221 acggactgct gaccgtccac gacgtgctgg gcaccgagcc gccctactac cagcgacgaa
     5281 tcgacgacaa cgtctggttc ggtttccctg ccgggaacga caggctgatg tacctgcaga
     5341 tcaaccgcca gctggagccc gaggacgggc ccgaatcgat cgacatcatt ctggactggg
     5401 cacggacgct gtgggaatcc gcggccgagg tgctcgtcat cgatctgcgc aacgacccgg
     5461 ggggaaacat cttcctgggg gacgcgatct cgggaatcct gcccgagctg tacgtgaacc
     5521 accctacgct gaaggccgtc gccgtcctgt tcggaacgga cacggtatcg gcggggacga
     5581 tcctggttgc ccagctcgag cgtacggtgc agccggtcct gatcggcgat ccgtccggct
     5641 ccgccccgaa catgttcctc cacgccgaga agatcgtcct gccgcattcc aacatcgagt
     5701 tcgaggtgtc gcgcgaggag tacgtcacga cccgcgacct cgacacgcgg cggtacatcg
     5761 tgccggacgt tccgatgtcg ctgtcgttcg ccgacttcgc gaacggacgg gacccgctgc
     5821 tcgacttcgc ggcgacgatc gacgacgcgc tcgcgcagaa gatttacggc gcggtcggac
     5881 tcgacgcgtg gcggagggac tctcagcgac cggcgctgcc ttcacacgca tcgcggaccg
     5941 ccgcggcacg ctgatcgtcg tcggccgtac ccggcggaaa ccgcgatcag agcgcggaca
     6001 cgcggtaggt cgccgtcggc gccgcgctgc acacctcgag ttcggcagcc gccgggtcgg
     6061 gaacgaaagc gacgccgtct cccgtcagca gcagccggcg cggagagagc gagatgcccg
     6121 acagccgctc gaccccgatc gccgcgaggg cgggcgacga gtgttgcagc ttgacgacgt
     6181 gagtcagcct cgcctcgacg cccggagccc cgacggtgcc gtcgagcgcc cagcgatgcg
     6241 tgaccgcgcc gtcgacatac tcgatcgggc gcatcgtgat ccgcgaattc gcttggtcga
     6301 cgacgatcgg atggccgctc gccgccaact cgacgagccc gccgatcgtc gggaggccga
     6361 ccgccgaacc gtcgacgatc gtgcagcctg gcggcggcgg cccggtcgtg acgacttcga
     6421 ccgaccacgt accggtcgcg tcgaataccc cgccgccggt cgtgccggag ccatcgtgac
     6481 aagcggcacc ggccagcagc agcggaacga cgatggcggt caggatgcgc atcggcctag
     6541 tcggattgtg ctgcgcgccg tccccgctgt caacgggcga tcgagcccgg cccgatcgag
     6601 cgcgtacagg tgatccgcgt gcctcagttc gggccctcga tccagacctc gccgccctcg
     6661 aagaactcct tcttccagat cggcacgcgc tgcttgagcc tgtcgatgat ccagcggcaa
     6721 gcgtcgaacg aggccgcacg atggggagcc gagaccgcga tcgccaccga ggcctcgccg
     6781 acttcgagct tgccgatgcg atgggcaatc gccacctcgc cgagcgcgaa ccgttcgagc
     6841 gccgcctggc gcagcgcgga catttcgcga agcgccatct cgtcgtaggc gtggtactcg
     6901 aggtgcagca cgcggcgccc ctgattctgc tcgcggaccg tgccgacgaa cagcgcgacg
     6961 gcgccgttac ccgggccgcc caccgcctgg agcagctcgg ccacatcgat cggccggcga
     7021 gcgagcagga tcatcgtcaa cccccgctga tcggcggcag gaacgcgacc tcccgcacgc
     7081 cgtccagcga ctcgctccaa tcgcaataac gcatgtcaca ggcgacgagc gggcggtagt
     7141 gcagcccggc cagcgcggca tgccggacgg acagcagctc ccacaacgcg ccgacgtcgg
     7201 accccgcgtc gatctcgatc gtctcgcggg gcgtgccggt cgtctcggcg agcgaggcga
     7261 agtaacgaac gtcgagcttc accgttccgc tcgcgcggga ccctggtcgg tcgcggcgac
     7321 catcgcggcc acgcgcccgg tgacgatccg catcgatcgg tcgagaccgc catccatcac
     7381 gatccccgag gcattgcggt gcccgcctcc gccgaactcg atcgccagcc gatgaacgtc
     7441 caacgtcccc ttcgagcgca gcgagacctt gacgcggccg tcggccagct cgcggtacaa
     7501 caccgccacg cgcacgccgt cgagcgccaa cagcgaggtg gtgatctccg tcacgtcgac
     7561 ctcctcggcg tcgagccgtt cgatcagctc gcgtgcgatc cgtaccgacg caatggcgcc
     7621 gccggcgtcc atccgcaggt cggtcaacgc gtggccgagc aaacggacgt aggacacgct
     7681 gttgcgttcg tagatctcgc ggtagacccg cgcggggtcg accccgagat cgatcagttc
     7741 ggcggcgatg cggtgcgcgc gcgggttcgt cgaattgaag cggaagaacc ccgtgtcggt
     7801 ggcgatgccg acgtagatcg cgagcgccgc cacctcgtcc ggcgcccagc cgacgccgcg
     7861 cgcgaggtcg tagatcatcg ccgtcgtggc gcagtagtgg acgtcgacga tgctgtcggc
     7921 ccacggtgca tcgcgcgtcg ggtggtgatc gatacacaac gtgcgcgcgg ccgacgcccg
     7981 catcacgcgc tccatccgtc cgagccggtc cggggccgag ttgtcgacca gcaggatacg
     8041 cccggccgac gcgatcaacg aatcgtgcgt cgccgcgtcg tagatctcga tcggtacgcg
     8101 gtcgaagccg acgaaccgca tcgactccgg cgcgcggtct tgattgacga tccgcacgcg
     8161 cttgccctgg tcgagcagat agctggccag cgcccattcg gaaccgacgg catcgccgtc
     8221 cgggttgatg tgcgtcacca gcacgaac
//
LOCUS       DASPGN010000501         5301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_209744, whole genome shotgun sequence.
ACCESSION   DASPGN010000501 DASPGN010000000
VERSION     DASPGN010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209744"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /gene="purH"
                     /locus_tag="VD788_14535"
     CDS             <1..1387
                     /gene="purH"
                     /locus_tag="VD788_14535"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HXV77527.1"
                     /translation="DGRVKTLHPRIHGGILAVRSNERHMADLERHEIAPIDLVVVNLY
                     PFEKTATTDGIDVRDVIEMIDIGGPAMVRAAAKNHAHVGVVVDPADYRRVLDEIESAG
                     GLSEATRLQLAAKAFRHTSEYDGAVHDYLSRLSPDGTLAAADSRLGDRLVFEFVKVDG
                     LRYGENPHQTAAFYRDPLVKEPSLAVARQLQGKELSFNNILDFDAALVLAAELPDGAC
                     VIVKHGNPCGTALGSEPRAAFERALACDPLSAFGGVIAFNRKVDLRAAQSVAEHFFEG
                     VAAPAFDEDARAALADKKKLRVLEVGDLARFERTGMDLRRVHGGLLVQSWDRIDEVVR
                     RCEIVTRRKPTEREWRALELAWIVAKNVKSNAIVYASEDRTLGVGAGQMSRVDAARIG
                     IDKAQGPLQGAVMASDAFFPFRDGIDVAAEAGIAAVIQPGGSIRDREVIEAADEHGMA
                     MVFTGRRHFRH"
     gene            complement(1407..3203)
                     /locus_tag="VD788_14540"
     CDS             complement(1407..3203)
                     /locus_tag="VD788_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77528.1"
                     /translation="MERDKQQLESLHQQASILYLQGEYLSALDAWSRLLEIDPADERA
                     REGARMCRALVQDQNGPAGATTATGGGPAADGGVGFGFGEDLDEELEEIDEMLAGGKP
                     NDWMDEKSQASPSGEETFEFDLSDVTDETRASIRSAAPSPDEFEFDVESTDRQATVDH
                     AVDSAFDSAPRAPVASPAPRAQKPPAAPAPPVDTGRMAAEELQNRTNELMAEALEFYE
                     QGDRAAALAALARLEILDEGNEAGKTFAAHIRAEMAQSRSSAAPPVPAEVPFEPEAPP
                     APVAGTQPSMPAVRSRPDPAPIARGPERRATPVVEPVASIDDFEEIEIPEAGPAGDEP
                     RSLDPPPRSSGSAVLLPSPPAVPRRWLLIVAGCLVVVASAFVALRFFGGGPSPDANAQ
                     TQLAAPPALSETAPPESAEPAPAAASEAEAPPPAPVAVDRAALDELVARGDDRIEAGD
                     FAAAVLAYNEALKLDPENEILRRRLREAGELFREQQATLEQRAAAIEAFNNGDYQNAL
                     RIFYRIPPVDDAERSRFTRYRVNGWYNMGLRALKSGDCRTARSHFEEARTIDALDPGL
                     RDAVELTATCFEGTTQSYFDVVRGLNYRGLDD"
     gene            complement(3304..3585)
                     /locus_tag="VD788_14545"
     CDS             complement(3304..3585)
                     /locus_tag="VD788_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77529.1"
                     /translation="MIRQLFLLLVALVAALWWSRRLFGATARRRPVPGGGAPSAGIRD
                     LGPMVRDRVCNTFLPKSRALRAGSGADEQFFCSEECRQKFRAGKTAHGA"
     gene            complement(3582..4205)
                     /locus_tag="VD788_14550"
     CDS             complement(3582..4205)
                     /locus_tag="VD788_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77530.1"
                     /translation="MKKENLAFLVGGLAFGFLVGFGAYHAVATTPQLEGAAAGTTMAG
                     PAGTPAPGAMPPAAVGAAPMVAEINRLKTLLQTEPNNFQALVRLGNLHYDVQMWEQAA
                     GYYERALAVESNPDLMTDLGVCYRNLGQFDRALDKFENAQQADPDHFQSLFNIVVVAG
                     LDLGDFVRAERALEVLEAMDPPPPRIDELRHAVEQSRASRADAGGES"
     gene            4279..>5301
                     /gene="rlmN"
                     /locus_tag="VD788_14555"
     CDS             4279..>5301
                     /gene="rlmN"
                     /locus_tag="VD788_14555"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXV77531.1"
                     /translation="MSRADVPRGKARTNLYGLDRPGLAQSVERGGLPVYHAGQIFRWI
                     YHRHELDPRNWTDLSNSLRERLALETVVDPGRIADRVTARDRTVKYRVVLPDGKAVEA
                     VYMEQRSRVTLCISSQVGCALACDFCLTGKMGLVRQLTPGEIVGQVALIRRERELDQR
                     AFNIVFMGMGEPLHNYDALTAAIRLLVDPQGFGLSRRRITVSTAGLAPAIERLGDERH
                     RPRLAVSLNATTDELRSRLMPINRRYPIARLLEACRRFADRSKERFTLEYVLLSGVND
                     GDDEIDRLAGIVRSVPAKLNLIPFNPVDEWLDYRPPERARVLRARDRLLAARVPVSIR
                     WSRGVEA"
BASE COUNT          766 a         1829 c         1901 g          805 t
ORIGIN      
        1 cgacggtcgg gtgaagacgc tgcacccgcg catccacggg gggatcctcg ccgtgcgctc
       61 gaacgagcgg cacatggccg atctggagcg gcacgagatc gccccgatcg acctggtcgt
      121 ggtcaacctc tacccgttcg agaagaccgc gacgacggac ggcatcgacg tgcgggacgt
      181 gatcgagatg atcgacatcg gcggcccggc gatggtgcgc gccgccgcga agaaccacgc
      241 acacgtcggg gtcgtggtcg acccggccga ctaccgccgc gtgctcgacg agatcgaatc
      301 cgcgggaggg ctgtccgagg cgacccggct gcagttggcg gccaaggcgt tccgccatac
      361 cagcgagtac gacggcgcgg tgcacgacta cctctcgcgt ctgtcgccgg atggcacgct
      421 cgcggcggcc gactcgcggc tcggcgaccg gttggtgttc gagttcgtca aggtcgacgg
      481 gctgcgctac ggcgagaacc cgcaccagac cgcggcattc tatcgcgacc cattggtcaa
      541 ggagcccagc ctggccgtcg cccgacagct tcagggcaag gagctgtcgt tcaacaacat
      601 cctcgacttc gacgccgcgc tggtgctggc cgcggagctt cccgacggcg cgtgcgtcat
      661 cgtcaagcac ggcaacccgt gcgggacggc gctcggctcc gagccgcgcg ccgcattcga
      721 gcgggcgctg gcctgcgacc cgctgagcgc cttcggcggc gtgatcgcgt tcaaccgcaa
      781 ggtcgacctg cgtgcggctc aatcggtcgc cgagcatttc ttcgaagggg tcgccgcgcc
      841 agcgttcgac gaggacgcgc gggccgcgct cgccgacaag aagaagctgc gggtgctcga
      901 ggtcggcgac ctcgcgcggt tcgagcgaac gggcatggac ctgcgtcggg tgcacggcgg
      961 gttgctggtg cagagctggg atcgcatcga cgaggtcgtt cgccgctgcg agatcgtcac
     1021 gcgccgcaag ccgaccgagc gggagtggcg tgcgctcgag ctcgcgtgga tcgtcgccaa
     1081 gaacgtcaag tcgaacgcga tcgtctacgc cagcgaagac cgcacgctgg gcgtgggagc
     1141 cggacagatg agccgcgtcg acgccgcgcg catcggtatc gacaaggcgc aaggcccgtt
     1201 gcagggcgcg gtgatggcgt ccgacgcgtt ctttccgttc cgcgacggga tcgacgtcgc
     1261 cgccgaagcc gggatcgcgg cggtgatcca gcccggcggg tcgattcgtg atcgcgaggt
     1321 gatcgaagcc gccgacgagc acggcatggc gatggtgttc accggccgcc ggcacttccg
     1381 tcactgatcc gcgagccgcg ccgcgctcag tcgtcgagtc cgcggtagtt caacccgcgc
     1441 acgacgtcga agtaggactg ggtcgtcccc tcgaagcagg tcgcggtgag ctcgaccgcg
     1501 tcgcgcaaac cggggtcgag cgcgtcgatc gtccgcgcct cctcgaagtg cgagcgggcg
     1561 gtccggcaat cgccggactt gagcgcgcgg agccccatgt tgtaccagcc gttgacgcga
     1621 taccgcgtga agcggctgcg ctcggcgtcg tcgaccggcg ggatacggta gaagatccgc
     1681 aacgcgttct ggtaatcgcc gttgttgaag gcctcgatcg cggcggcgcg ttgctcgagt
     1741 gtggcctgct gttcgcggaa cagctcaccc gcctcgcgca atcggcgccg gaggatctcg
     1801 ttttccgggt cgagcttcaa cgcctcgttg taggccagga ccgcggcagc gaaatcgccg
     1861 gcctcgatcc ggtcgtcacc gcgggccacg agctcgtcga gggccgcccg gtcgaccgcg
     1921 acgggggccg ggggcggtgc ttccgcttcg ctggccgcag ccggcgccgg ctcggcgctc
     1981 tccggcggcg cggtctcgct caacgccggc ggtgcggcga gctgggtctg ggcgttcgcg
     2041 tcgggcgacg gtccgccgcc gaagaagcgg agcgcgacga acgcgctcgc gacgacgacg
     2101 aggcaaccgg cgacgatcaa cagccaccgt cgcgggaccg ccggaggcga cggcagcagg
     2161 accgcgctgc ccgacgaacg gggcggcggg tcgagtgacc gcggctcgtc gcccgccggg
     2221 ccggcctcgg ggatctcgat ctcttcgaag tcgtcgatcg acgcgaccgg ctcgacgacc
     2281 ggggttgcgc ggcgctccgg gccacgtgcg atcggcgcgg ggtcgggtcg cgatcgtacc
     2341 gcgggcatcg acggttgcgt cccggccacc ggcgcgggag gggcctccgg ctcgaacggc
     2401 acctcggcgg gcaccggcgg cgccgcgctc gaccgcgact gcgccatctc ggcgcggatg
     2461 tgcgcggcga aggtcttgcc cgcctcgttg ccctcgtcga ggatctcgag ccgggccagc
     2521 gcggccaacg cggccgctcg atcgccctgc tcgtagaact cgagcgcttc ggccatcagc
     2581 tcgttggtgc ggttctgcag ctcctcggcc gccatccggc ccgtgtcgac ggggggagcc
     2641 ggcgctgccg gcggcttctg ggcgcggggt gcgggtgacg ccaccggggc ccggggggcg
     2701 gagtcgaatg ccgagtcgac cgcgtggtcg accgtcgcct gccggtcggt actctcgacg
     2761 tcgaactcga actcgtcggg cgacggggct gcggaacgga tcgacgcgcg agtctcgtcg
     2821 gtcacgtccg acagatcgaa ctcgaacgtc tcctcgcccg acggactcgc ctgcgacttt
     2881 tcgtccatcc agtcgttggg cttgccgccg gccagcatct cgtcgatttc ctcgagctct
     2941 tcgtcgagat cctcgccgaa accgaaaccg acgccgccat cggcggccgg gccgccgccg
     3001 gtcgccgtgg tcgcgcccgc cggcccgttc tggtcctgga ccaacgcccg gcacatgcgc
     3061 gcaccctcgc gcgcgcgctc gtcggcggga tcgatctcga gcaggcggga ccatgcgtcg
     3121 agcgccgaga gatactcgcc ctgcaggtag agaatcgagg cttgctggtg caacgactcg
     3181 agctgttgct tgtcgcgttc catcgttccc tgcggaagcc ggtcggcgca caactgcagc
     3241 gcaaacccag ggttccctcc cggcgcaata tagggcccgc gcccggcgcg ggcaattcac
     3301 cggtcaggcg ccgtgggccg tcttgccggc gcggaacttc tgccggcact cctccgagca
     3361 aaagaactgc tcgtcggctc ccgagcccgc gcgaagggca cgcgatttgg gcaggaacgt
     3421 gttgcagacg cggtcgcgaa ccatcgggcc gaggtcgcgg atgccggccg acggggcccc
     3481 accgcccggg accggtctgc gccgggcggt ggccccgaac aaccggcgcg accaccacag
     3541 cgccgcgacc aacgcgacca acagcaggaa gagctgccgg atcacgactc gcctcccgcg
     3601 tcggcccggc tggcgcgcga ctgttcgacg gcgtgtcgta gttcatcgat gcgcggcggc
     3661 ggcggatcca tcgcctccag cacctcgagc gcccgctcgg cacggacgaa gtcgccgagg
     3721 tcgaggccgg ccacgacgac gatgttgaac agcgactgga agtggtcggg gtcggcctgc
     3781 tgcgcgttct cgaacttgtc gagtgcgcgg tcgaactgcc cgaggttgcg atagcacacg
     3841 ccgaggtcgg tcatcaggtc cgggttgctc tcgacggcga gcgcccgttc gtagtagccg
     3901 gcggcctgct cccacatctg aacgtcgtag tgcaggttgc cgaggcgcac cagcgcctgg
     3961 aagttgttcg gttcggtctg cagcagcgtc ttgagccggt tgatctcggc gaccatcggc
     4021 gcggcgccca cggcagccgg cggcatcgcg ccgggggccg gcgttcccgc cggcccggcc
     4081 atcgtcgtgc cggctgccgc gccttcgagc tgcggcgtgg tcgcgacggc gtggtaggct
     4141 ccgaatccga cgaggaatcc gaaggccagc ccgccgacga ggaatgccag gttttccttt
     4201 ttcatcgacc gttcgacgtt agccgagccg cttcgtaccg tcaagctgcc gcggccgtgt
     4261 gctagagtcc ggcgcgtcat gagccgcgca gacgtgcccc gcggcaaggc ccgcaccaat
     4321 ttgtacgggt tggatcgtcc cggcctggcg caatcggtcg aacgcggcgg gctgcccgtg
     4381 tatcacgccg gtcagatctt ccgctggatc taccaccgac acgagctcga cccgcggaat
     4441 tggacggatc tgtccaattc gttgcgcgag cggctggcgc tcgagaccgt cgtcgatccc
     4501 gggcggatcg ccgaccgcgt caccgcccgc gaccgcaccg tcaagtaccg cgtcgtgctg
     4561 cccgacggca aggcggtcga agcggtctac atggagcaac gctcgcgggt gacgctgtgc
     4621 atctcgagcc aggtcggctg cgcgctcgct tgcgatttct gcctgaccgg caagatgggc
     4681 ctggtgcgcc agctgacccc cggcgagatc gtcggccagg tcgcgctgat tcgccgggag
     4741 cgcgagctcg accaacgcgc attcaacatc gtgttcatgg ggatgggcga gccgctgcac
     4801 aattacgacg cgctgacggc ggcgatccgg ctgctggtcg atccgcaggg gttcggtttg
     4861 tcgcgtcgac ggatcaccgt ctcgaccgcc gggctggctc cggcgatcga acggctcggc
     4921 gacgagcggc accggccgcg gctggccgtc tcgctcaacg cgacgaccga cgagctgcgc
     4981 tcgcgactga tgccgatcaa ccggcgctac ccgatcgccc ggctgctcga ggcctgtcgc
     5041 cgcttcgccg accgatcgaa ggaacggttc acgctggagt acgtgctgtt gtccggcgtg
     5101 aacgacggcg acgacgagat cgaccgtctg gccgggatcg tccgcagcgt cccggccaag
     5161 ctgaacctga tcccgttcaa tccggtcgat gagtggctgg actaccggcc gcccgaacgg
     5221 gcgcgggtgc tgcgcgcgcg cgaccggttg ctcgccgcgc gcgtgccggt gagcatccgc
     5281 tggagccgag gggtcgaggc a
//
LOCUS       DASPGN010000502         3420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1263017, whole genome shotgun sequence.
ACCESSION   DASPGN010000502 DASPGN010000000
VERSION     DASPGN010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263017"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VD788_14560"
     CDS             <1..716
                     /locus_tag="VD788_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77532.1"
                     /translation="NVGPGTRNCLDDADCGVGNTCVDKPLDYGISSGDVNKVHFDPGW
                     LADGNLFGNQSLACATALPIRELERDPALGFQTVALDPRPAPGSPLLAGGGGKAADPG
                     VTPAAHQGGFGSSNWADDWTQSSRNGYYSKCGIGGSGTAVPDEVAELSFRGKDELRWT
                     KVGDSVTYDVLRSTTADSFAAATSLEIPPGDNVHNRQADASASPGVNTTYYFVVRALN
                     DCGNGSIGTFSNGAERIAP"
     gene            812..2029
                     /locus_tag="VD788_14565"
     CDS             812..2029
                     /locus_tag="VD788_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77533.1"
                     /translation="MYEQHMDGRWRWPAVCGGVLIAVAVGGTAAREQTTPAPGERALD
                     YAFRFAGAVDDAKDRAKAQESAINGYVDAGLLDEAELRAAEIEGWRRGVVLGRLAALR
                     LREGDAERARGLLAAAERSAGEVQDWGRDRILAQVAEVRALLGEDERAREIGEQLIRN
                     DPRTYEGRATVALASALAARGDLAGAQRTIGTLDDSVDLYVAWWRTEGYLDLAERAGV
                     DPSQRAELLDRARLSARAIVGWKQAEMLQRIAAAEQRAGRDQDALESLERAERIVLDL
                     PRTMSLRGPLLADLARSWVATGRREHALELVGTAERDLDYVASIVERPAAIARVATGY
                     VAVGRDGDGVRLLRLALARAQALENPRPRALALSAVCREAGRHGVEFEEDLTPRLDAM
                     LAELTGETVRAGL"
     gene            2160..>3420
                     /locus_tag="VD788_14570"
     CDS             2160..>3420
                     /locus_tag="VD788_14570"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXV77534.1"
                     /translation="MLCAIAAILVSSSPVRAQTGSAGGVRGDVVDADFSAPVAGARVT
                     LVGTTLSATTEPDGRFVIQNVPPGEYTLTIAKEGFERRVVSGVIVTSGSLADLGTVEL
                     DVEIYEMDELVVTGEDLLAGSEIALLEVREVAVQLQDNISADLLNKAGASDAAGALKL
                     VTGTSVAEGKYATVRGLSDRYTGTTLNGVRLPASDDRRRAVQVDIFPSGTIESISVTK
                     TFTPDLQGDFTGGGVDIQTRSMPDRLILAGSYGREWDAIVTENENFLTYEGGGVTALA
                     RPEQDHDIPVARGSTFPLIGAAPPRPIPTPDAQAAADELDRLTRLFDPTMGVSYTEQD
                     PGGSASLLVGSPFSVGRQAKLGVLAAVTARHTYELYENAANNDARVDTITPPDQPVPL
                     GTVRDDSRGVDEALLGFLGKLTFRPTLN"
BASE COUNT          509 a         1174 c         1252 g          485 t
ORIGIN      
        1 cgaacgtcgg gccgggtacg cgcaactgtc tggacgacgc cgactgcggc gtgggcaaca
       61 cctgcgtcga caagccgctc gactacggca tttcgagcgg cgacgtgaac aaggtccact
      121 tcgatcccgg ctggctcgcc gacggcaact tgttcggcaa ccagtcgctg gcctgcgcca
      181 ccgcgctgcc gatccgagag ctcgagcgcg atccggcgct cggcttccag acggtggcgc
      241 tcgacccgcg cccggcgccc ggcagcccgc tgctggcggg tggcggcggc aaggcggccg
      301 atcccggagt cacgccggcg gcgcaccagg gtgggttcgg cagctcgaac tgggccgacg
      361 attggaccca gagctcgcgc aacggctact actcgaagtg tgggatcggc ggatccggca
      421 ccgcggtccc cgacgaggtc gcggagcttt cgttccgcgg caaggacgag ctgcgctgga
      481 ccaaggtcgg cgactcggtc acctacgacg tgctgcgctc gacgacggcc gactcgttcg
      541 ccgcggcgac ttcgctcgag atcccgcccg gcgacaacgt gcacaaccgc caggccgacg
      601 cctcggcgag cccaggcgtg aacacgacct actacttcgt cgtgcgggcg ctgaacgact
      661 gcggcaacgg ctcgatcggc actttctcga acggtgccga gcgcatcgct ccgtgagtcg
      721 ggtgaacgga ccgagctgaa cgagggttag agaagggcgg gggctccgat ccgcgggccc
      781 ccgcctccgt tccagacggg gagaccacgg aatgtacgaa cagcacatgg acggccggtg
      841 gcgctggccg gcggtctgcg gtggggtgct gatcgcggtg gcagtcggag ggactgccgc
      901 gcgcgagcag acgacgcccg cgccgggcga gcgggcactc gactatgcct tccgcttcgc
      961 cggagcggtc gacgatgcga aggaccgggc caaggcgcag gagtcggcga tcaacggcta
     1021 tgtcgacgcc ggtctgctcg acgaggccga gcttcgggcg gccgagatcg agggctggcg
     1081 gcgcggcgtg gtgttggggc ggctcgccgc cctgcggctt cgcgagggag acgcggagcg
     1141 cgcgcggggg ctgttggccg ccgccgagcg gagcgccggc gaggtgcagg actggggccg
     1201 agacaggatc ctggcgcagg tggccgaggt ccgcgcattg ttgggcgagg acgaacgcgc
     1261 gcgggagatc ggcgagcagc tgatccggaa cgacccgcgt acgtacgagg gacgcgccac
     1321 ggtcgcgctg gcgtcggcgc tcgccgcgcg cggggacctg gccggcgcgc agcggacgat
     1381 cggtacgctc gatgacagcg tcgacctgta cgtcgcctgg tggcgcaccg agggatatct
     1441 cgatcttgcc gagcgtgccg gcgtcgatcc gtcgcagcgt gccgaactgc tcgatcgggc
     1501 ccgcctctcc gcgcgggcga tcgttggctg gaagcaggcc gagatgctgc aacgaatcgc
     1561 cgcggccgag cagcgtgcgg gtcgcgacca agacgcgctc gagagcctcg agcgtgccga
     1621 gcggatcgtg ctcgatcttc cacggacgat gtcgctgcgc ggaccgttgc tcgccgacct
     1681 ggctcgatcg tgggtcgcga ccggtcgtcg cgagcacgcg ctcgagctgg ttgggaccgc
     1741 cgagcgcgat ctggactacg tcgcgagcat cgtcgagcgc ccggccgcga tcgcgcgcgt
     1801 ggcgacgggc tacgtggcgg tcggtcgcga cggcgacggg gtgcggctgc tgcggctcgc
     1861 gctggctcgt gctcaggcgc tcgaaaaccc gcgaccacgg gcgctggcgc tgtcggcggt
     1921 ctgccgcgag gcgggacggc acggcgtcga gttcgaggag gacctgacgc cgcgtctcga
     1981 cgccatgctc gcggagttga ccggggagac ggtgcgggcc ggtttgtgac acttcggtga
     2041 cggatccgcc gttgtcttga cgcccttcga gcacggttcc tacgatggcg cctctcgacc
     2101 gaacggttct cgcgaggcgc gcgtcccagg tgctccaccc caaacgaccg ttactctccg
     2161 tgctctgcgc gatcgccgcg atcctcgtct cgtcgagtcc cgttcgggcg caaacgggct
     2221 ccgccggtgg cgtccgcggc gatgtcgtcg acgccgattt ctccgccccg gtcgccggcg
     2281 cgcgcgtgac gctcgtcggc acgacgctgt ccgcgacgac cgagcccgac gggcgcttcg
     2341 tcatccagaa cgtgcctccg ggcgagtaca cgttgacgat cgccaaggag ggattcgagc
     2401 gccgagtggt gtcgggcgtc atcgtcacgt cgggaagtct ggccgacctg gggacggtcg
     2461 agctcgacgt cgagatctac gagatggacg agctcgtcgt caccggcgag gacctgctcg
     2521 cgggcagcga gatcgcgctg ctcgaggtgc gggaggtcgc ggtccagctg caggacaaca
     2581 tctcggcgga cctgctcaac aaggccggtg cgagcgatgc ggccggcgcg ctcaagctcg
     2641 tgaccggcac ctcggtcgcc gagggaaagt acgccaccgt gcgcggtctt agcgaccggt
     2701 acaccggcac gacgctgaac ggcgtaaggc tgccggcgtc ggacgaccgc cggcgcgcgg
     2761 tgcaggtcga catcttcccc agcgggacga tcgagagcat ctcggtgacc aagacgttca
     2821 cacccgacct gcagggcgac ttcaccggcg gcggcgtcga catccagacc cgatcgatgc
     2881 cggaccggct gatcctcgcc ggctcgtacg gacgggaatg ggacgcgatc gtcacggaga
     2941 acgagaactt cctgacctac gagggaggtg gcgtgaccgc gctggcgcgg cccgagcagg
     3001 accacgacat cccggtcgcg cgcggctcga ccttcccgct gatcggcgcg gctcccccgc
     3061 gaccgatccc gacgcccgac gcccaggccg cggcggacga gctcgatcgt ctgacccgct
     3121 tgttcgaccc gacaatgggc gtctcgtaca cggagcaaga cccgggcggc tcggcctcgc
     3181 tgctcgtcgg cagtccgttc tccgtcggcc ggcaggccaa gctcggcgtg ctcgccgccg
     3241 tcaccgcgcg ccacacctac gagttgtacg agaacgccgc gaacaacgac gcccgcgtcg
     3301 acacgatcac gccgcccgat caacccgtcc cgctcggcac cgtgcgcgac gattcgcgcg
     3361 gcgtcgacga ggcgctgctc ggcttcctgg gcaagctgac cttccgcccg acgctgaacc
//
LOCUS       DASPGN010000503         6122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_560962, whole genome shotgun sequence.
ACCESSION   DASPGN010000503 DASPGN010000000
VERSION     DASPGN010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560962"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1609)
                     /locus_tag="VD788_14575"
     CDS             complement(<1..1609)
                     /locus_tag="VD788_14575"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXV77535.1"
                     /translation="MASITDLAIRRPVATKMVYLMLIVIGIVSFRTLPVDLLPEIEFT
                     QLTVRTRYPNVGPAEIEQIITDPIENAVSGLPDLERITSQSEEGSSRVRLEFGRGTDI
                     DEAANDLRAALDELRDQFPLEAEPPEIWKFDLDQIDVVTLAATSTRPLDELTRILEDE
                     LAQHFEQIPGVGSIELRGGIYREIRIDLDRDRLKAADLTALDVRDALVGANVTLAGGN
                     VKSGFADLYVRPLGEFRTLDEIRRVVVRQSGGYSVRVRDVAEVVDGFENVQYLSEING
                     VPSVTLSIQKQSGANTVEVAERILTEARRINAERDDVELTVFSDQSEFIRQSIASVRN
                     AALWGSLFAVLLLYLFVRSRSGTVIIATAIPISVIASFGLLFVGGQTLNQMTFGGLAL
                     GVGMIVDNAIVVLESIVRKREEHDESPRHAALHGTRDVAGAVVASTLTSCVVFLPVVF
                     TRSTSGALFQALALVVVFALTCSLLVALTLVPMLSAANLRLASSDERARSRAARFFRE
                     LESRYTAWLRVAIRHRRLTFGVTAAALA"
     gene            complement(1610..2965)
                     /locus_tag="VD788_14580"
     CDS             complement(1610..2965)
                     /locus_tag="VD788_14580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXV77536.1"
                     /translation="MGSRWIYPASAALALACMACAGQNAAAPDRPAIESPLVEALPAQ
                     SGSLPLVERLSGIVNAENQVAVRAEIDAPILEVLVRSGDAVALGQPLVRLESTSLRER
                     LRQEEANLRLAEATAAEAAAQRDELGARVVRARALAGQSLISEQELDTLEAQLTAAHA
                     ATAQAEASVDEARAIVEQARTAASKTVVRSPVSGHVGRREAEVGMIATPATPLFTVGN
                     LDSLIVEVQLTEEMLGDLRPGQTARVHSPALADEPIVASLSRISPFLAPGSFSTTGEI
                     DVTNSGDRLKPGMFVTVDVLYGESERATLVPSSALWEEPTTGQWGVYVVASSARTADG
                     AADADAQPRAVEFRPVAVLAEGGERAGVSGVEPGEWVVVLGQHLLRPTDDATARVRVT
                     SWERVLELQRLQREDLLRGFLDKQQRWAREHGAAPPTNEEFLGGEIPAGAPAAGAGGG
                     R"
     gene            3167..4771
                     /locus_tag="VD788_14585"
     CDS             3167..4771
                     /locus_tag="VD788_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXV77537.1"
                     /translation="MGERLLLGDEAVAQAAVDAGVAGAFSYPGTPATEVFEYIEHGAA
                     ARGMVSARWSANEKVAYEEALGMSYAGRRAIVSMKHVGLNVAADPFVNSALTGVNGGL
                     LLVVADDPGMHSSQNEQDSRFYGEFAQVPIFEPATQQQAYDMTLEAFEYSERVGLPVM
                     LRLVTRLSHSRANVALREAGRPAAGEARPLPSPDDWTLVPVVARRRFRGLVELQPRLV
                     DDATRSTANRLELAGPRGVIAAGIARNYVAEAAAGRSELSLLELGRYPIPVALVRKLV
                     DHCDEILVVEEGYPWIERRLNGLLGVPGKAIRGRLTGEIPATGELTPETVAAALGYAA
                     AEGEPPPVDLAGRPPQLCQGCPHGDSFRAIVEACSIYERPILFGDIGCYTLGVAPPYR
                     AVHACVDMGASIAMAHGASQTGIRPVICTIGDSTFSHSGMTGLLGAIRSDADMTVFVL
                     DNGTVAMTGTQPSFTTGERLLDVLRGLGVDPRHLHSIDPLPQRHADNVELIRREIEHR
                     GLSVIIPNRACIHLHKKRRESIAVTA"
     gene            4781..>6122
                     /locus_tag="VD788_14590"
     CDS             4781..>6122
                     /locus_tag="VD788_14590"
                     /inference="COORDINATES: protein motif:HMM:NF005324.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HXV77538.1"
                     /translation="MSVANESRVSLKQNLLLSGVGGQGILAIAQAISHAALARGLRIK
                     QAEVHGMAQRGGGVQSHLRISDGEIASDLIPRGQADLLIAVEPLEALRQLDFLSPAGA
                     LVTSVNPFVNIDNYPPVESLLERIARHPRHIMIDADRIARAAGSARTANVVMLGAASL
                     WIDLEPGELERSAVEIFADKGDRVVEVNRRAFRFGRNAARAYVDGIERGGKSIAVRHW
                     IETLSAEHLAQPERPDAPLFDMLSVGDRLSGAEQHAVERALESTLESGRLQLFEHEVY
                     NIVQLVGAISPPHHVFVSTEELIAPATLRHVPGDRVVVKLVSPEVVHKTEASGIVFCR
                     KDIDVVRGEIDRLIARHRRSVDVRGVLVVEHVERVRPGFGNELFVGIRSTREFGPVIA
                     AGLGGIDTEYLADKMKPGLAVAKAPALLTTPEAFLELFSRTAAYEILSGRARGRS"
BASE COUNT          914 a         2171 c         2124 g          913 t
ORIGIN      
        1 cggcgagcgc cgccgcggtc acgccgaacg tcagccggcg gtggcggatc gcgacgcgca
       61 accaggcggt gtagcgcgac tcgagctcgc ggaagaagcg cgcggccctc gagcgcgcgc
      121 gctcgtcgga cgaggccagc ctgaggttcg cggccgacag catcggcacg agcgtcagcg
      181 cgaccagcaa cgagcaggtc aaggcgaaga cgacgaccag cgccagcgcc tggaacagcg
      241 cgccgctcgt cgaacgcgtg aagaccaccg gcaggaagac cacgcacgag gtcaacgtcg
      301 aggcgacgac ggcgcccgcg acgtcgcgcg tgccgtgcaa cgcggcgtgc cgcggcgact
      361 cgtcgtgctc ctcgcgcttg cgcacgatgc tctcgagcac cacgatcgcg ttgtcgacga
      421 tcatgccgac gccgagcgcc aaaccgccga acgtcatctg gttgagcgtc tgtccgccga
      481 cgaacaacag cccgaagctc gcgatgaccg agatcggaat cgcagtggcg atgatcaccg
      541 tgccgctgcg gctgcgcacg aacagataga gtagcagcac ggcgaacagc gagccccaca
      601 gcgcggcgtt gcgcacgctg gcgatcgatt gacggatgaa ctcgctctgg tcggagaaga
      661 cggtcagctc gacgtcgtca cgctccgcgt tgattcgccg cgcttcggtc aagatccgct
      721 cggcgacctc gacggtgttg gcgccgctct gcttctgaat cgacagcgtg accgacggga
      781 cgccgttgat ctcggacaag tactgtacgt tctcgaagcc gtcgacgacc tcggccacgt
      841 cgcgcacgcg caccgagtag ccgccggact ggcggacgac gacgcgacga atctcgtcga
      901 gcgtgcggaa ctcgccgagc gggcgcacgt acaggtcggc gaatccgctc ttgacgttgc
      961 cgccggccag cgtcacgttc gcgccgacga gcgcgtcgcg tacgtcgagc gccgtcaggt
     1021 cggccgcctt caaccgatca cggtcgaggt cgatgcggat ctcgcggtag atcccgccgc
     1081 gcagctcgat cgacccgacg ccggggatct gctcgaagtg ctgcgccagc tcgtcctcga
     1141 ggatccgcgt cagttcgtcg agcgggcgcg tcgatgtcgc tgccagcgtg acgacgtcga
     1201 tctggtccag gtcgaatttc cagatctcgg ggggttcggc ctcgagcggg aactggtcgc
     1261 gcagctcgtc gagcgcggcg cgcaggtcgt tggccgcttc gtcgatgtcg gtcccgcggc
     1321 cgaactccag ccggacgcgg ctcgagccct cctccgattg cgacgtgatc cgctccaggt
     1381 ctggcagccc gctgaccgcg ttctcgatcg ggtcggtgat gatctgctcg atctcggccg
     1441 gtccgacgtt gggataacgc gtccgcaccg tcagctgggt gaactcgatc tcgggcagca
     1501 ggtcgaccgg cagcgtgcgg aacgagacga tgccgatcac gatcagcatc aggtagacca
     1561 tcttcgtcgc cacgggccgg cggatcgcca ggtcggtgat cgacgccatc tagcggcccc
     1621 caccggcgcc agcggccggc gcgcccgcgg ggatctcacc gccgaggaac tcttcgttgg
     1681 tcggcggtgc cgcgccgtgc tcccgtgccc agcgctgctg tttgtcgagg aagccgcgca
     1741 gcaggtcctc gcgctgaagc cgctgcagct cgagcacccg ctcccacgac gtcacgcgca
     1801 cgcgggccgt cgcgtcgtcg gtcgggcgta gcaggtgctg tccgagcacg acgacccact
     1861 cgcccggctc gacgccgctg acgccggccc gctcacctcc ctcggcgagc acggcgaccg
     1921 gtcggaactc gaccgcgcgc ggctgtgcgt cggcgtccgc cgcgccgtcg gcggtgcgag
     1981 cggaactcgc cacgacgtag acgccccatt ggccggtcgt cggctcctcc cacagcgcgc
     2041 tcgacggcac gagcgtcgcc cgctcgctct cgccgtacag tacgtcgacc gtgacgaaca
     2101 tccccggttt caaccgatcg ccgctgttcg tgacgtcgat ctcgccggtg gtgctgaagc
     2161 taccgggtgc gaggaacggc gagatgcgcg acaacgacgc gacgatcggc tcgtccgcca
     2221 gcgccggcga gtgaacccgc gcggtttggc cggggcgcag atcgcccagc atctcctcgg
     2281 tcagttggac ctcgacgatc agcgagtcga ggttgccgac ggtgaacagc ggggtcgcgg
     2341 gcgtggcgat cattcccacc tccgcctccc gtcgcccgac gtgaccggag accggcgagc
     2401 gcacgaccgt cttcgacgcg gcggtgcgcg cctgttccac gatcgcgcgc gcctcgtcga
     2461 cgctggcctc ggcctgggcc gtggcggcgt gggccgcggt cagctgggct tcgagcgtat
     2521 cgagctcctg ctcgctgatc agcgactggc cggccagcgc ccgcgcccgt acgacacgcg
     2581 ccccgagctc gtcgcgttgg gcggccgctt cggcggccgt cgcctcggcc agccgcaggt
     2641 tggcctcctc ctgccgcagc cgctcccgca gcgaggtgct ttcgagacga acgagcggct
     2701 gacccagcgc gaccgcgtca ccgctgcgca ccagcacctc gaggatcggc gcgtcgatct
     2761 cggcgcggac ggcgacctgg ttctcggcgt tgacgatccc gctcaaccgc tcgacgagcg
     2821 gcaacgagcc actctgtgcc ggcagcgcct cgacgagcgg ggattcgatc gccggccgat
     2881 ccggggccgc ggcgttctgg ccggcacacg ccatacaggc cagcgccagc gccgcgctcg
     2941 cgggatagat ccaccggctg cccatctccg cgatcccgga cccccttccc ccttcaacct
     3001 aacaccgaac cgcccgcgtc acgaatcgcg cggaggtacg gccggtgggc ccgctgccca
     3061 acgcgacgaa taggaccgac tttccccgct ggcgcccgtg tcgcccgact cgcccggcga
     3121 ctagcatctc cccgaggaag ctcgcgagcg cgcgggggag acggcgatgg gcgaacggct
     3181 gttgctgggc gacgaggcgg tggcgcaagc ggcggtcgac gccggcgtgg cgggcgcgtt
     3241 ctcctatccg ggtacgccgg cgaccgaggt gttcgagtac atcgagcacg gtgcggccgc
     3301 ccgcggcatg gtctcggcgc gctggtcggc caacgagaag gtggcctacg aggaggcgct
     3361 cggcatgtcc tatgccgggc gccgcgcgat cgtgtcgatg aagcacgtcg gcttgaacgt
     3421 cgccgccgat ccgttcgtca actccgcgct gaccggcgtg aacggcggcc tgctgttggt
     3481 cgtggcggac gatcccggca tgcactcgtc gcagaacgag caggacagcc gcttctacgg
     3541 cgagttcgcc caggtgccga ttttcgagcc ggccacgcag cagcaggcct acgacatgac
     3601 gctcgaggcg ttcgagtact cggagcgcgt cgggctgccg gtgatgctgc gtcttgtgac
     3661 gcgcctgtcg cacagccggg ccaacgtggc cctgcgcgag gccgggcggc ccgcggcggg
     3721 cgaggcccgc ccattaccga gccccgacga ttggacgctc gtaccggtcg tcgcccggcg
     3781 gcgcttccgc gggctggtcg agctgcaacc gcggctggtc gacgacgcga cgcggtcgac
     3841 ggcgaatcgc ctcgagctcg cggggccgcg cggcgtgatc gcggccggca tcgcccgcaa
     3901 ctacgtcgcc gaagcggcgg ccggccgcag cgagctgtcg ctgctcgagc tcggccgcta
     3961 tccgatcccg gtcgcgctcg tgcgaaagct ggtcgatcac tgcgacgaga tcctggtggt
     4021 cgaggagggc tatccgtgga tcgaacgccg gctcaacggc ctgctcgggg tcccgggcaa
     4081 ggcgatccgc ggaaggctga ccggggagat accggcgacc ggcgaactga ctcccgagac
     4141 cgtcgccgcc gcgctcggct acgccgccgc cgaaggcgag ccgccgcccg tcgacctcgc
     4201 cgggcggccc cctcagctgt gccagggctg ccctcacggc gacagcttcc gcgcgatcgt
     4261 cgaggcctgc tcgatctacg agcggccgat cctgttcggc gacatcggct gctacacgct
     4321 gggcgtcgct ccgccgtacc gcgcggtcca cgcctgcgtc gacatgggtg catcgatcgc
     4381 gatggcccac ggcgcgagcc agaccggtat tcgcccggtc atctgcacca tcggcgattc
     4441 gaccttctcg cactcgggca tgaccggcct gctcggcgcg atccgcagcg acgccgacat
     4501 gacggttttc gtgctggaca acggcacggt cgcgatgacc ggaacccagc cctcgttcac
     4561 gacgggtgag cgattgctcg atgtgttgcg cggactgggc gtcgatccgc gtcacctaca
     4621 ctcgatcgac ccgctgccgc agcgccacgc cgacaacgtc gagctgatcc gccgcgagat
     4681 cgagcaccgc gggctgagcg tgatcatccc caaccgggcc tgtatccacc tgcacaagaa
     4741 acgacgggaa tcgatcgccg tgacggcgtg agacgggacg atgagcgtag cgaacgagag
     4801 ccgggtctca ctgaagcaga acctgctgct gtcgggtgtc ggcgggcagg ggatcctcgc
     4861 catcgcacag gcgatctcgc acgccgcgct cgcacgcggg ctgcggatca aacaggccga
     4921 ggtgcacggc atggcgcaac gtggcggcgg cgtgcagtcc cacctgcgga tctccgatgg
     4981 tgagatcgcg agcgacttga tcccgcgcgg gcaggccgac ctgttgatcg cggtcgagcc
     5041 gctcgaggcc ttgcgccagc tcgacttcct ctcccccgcc ggcgcgctgg tcaccagcgt
     5101 caacccgttc gtcaacatcg acaactaccc gcccgtcgag tcgttgctcg agcggatcgc
     5161 acgccatccg cgtcacatca tgatcgacgc cgaccggatc gcgcgggcgg cgggctcggc
     5221 gcggacggcc aacgtcgtga tgctcggtgc ggcatcgctg tggatcgacc tcgagccggg
     5281 cgagctcgag cgttccgccg tcgagatctt cgccgacaag ggcgaccgcg tcgtcgaggt
     5341 gaaccggcgc gctttccgct tcggtcgcaa cgcggcccgg gcgtacgtcg acgggatcga
     5401 gcgcggcggc aagtcgatcg cggtccgcca ctggatcgag acgctgtcgg ccgagcatct
     5461 cgcgcagccg gagcggcccg acgctccgct gttcgacatg ctgtcggtcg gcgaccggct
     5521 gtcgggcgcc gagcagcacg cggtcgagcg cgcgctcgaa tcgacgctcg agtcgggtcg
     5581 actgcagctg ttcgagcacg aggtgtacaa catcgtccag ctcgtcggcg cgatctcacc
     5641 gccgcaccac gtcttcgtat cgaccgagga gctgatcgcg cccgcgacgc tgaggcacgt
     5701 tcccggcgac cgcgtcgtgg tcaagctcgt ctcgcccgag gtcgtgcaca agaccgaggc
     5761 cagcggcatc gtcttctgcc gcaaggacat cgacgtcgtc cgcggcgaga tcgaccggct
     5821 gatcgcacgc caccgccgat cggttgacgt gcgcggcgtg ctcgtcgtcg aacacgtcga
     5881 gcgtgtgcgg ccgggtttcg gcaacgagtt gttcgtcggc atccgctcga cgcgtgagtt
     5941 cggtccggtg atcgcggcgg ggttgggcgg gatcgacacc gagtacttgg cggacaagat
     6001 gaagcccggc ctcgcggtgg cgaaggcacc tgcgctgttg accacgcccg aggcctttct
     6061 cgagctgttc tcgcgcacgg cggcgtacga gatcctgtcg ggacgggccc gcggacggag
     6121 cc
//
LOCUS       DASPGN010000504         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1497286, whole genome shotgun sequence.
ACCESSION   DASPGN010000504 DASPGN010000000
VERSION     DASPGN010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497286"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VD788_14595"
     CDS             complement(<1..516)
                     /locus_tag="VD788_14595"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXV77539.1"
                     /translation="MPSDETTPSDHRASRFLAAVPAALLCGGGSFPCSAHDLSYAGTL
                     LEGEIPIPEAPEIGLTLCSAAGDIELRLSGRVAHAHRLDDSRTVRLGVQFDRLDGDQR
                     EALERLLSRVVEGMAPAAIEAIDENASIDEIRAALALITTAHRTALASRCGPRERKLL
                     RYDTDPHVLDGL"
     gene            663..1724
                     /locus_tag="VD788_14600"
     CDS             663..1724
                     /locus_tag="VD788_14600"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXV77540.1"
                     /translation="MSLQFSLPVTETILSVISQADQMRGKWSAAIPIGEGRLQRIREA
                     TAIRSIGSSCRLSGIGISDHDVAGILRGQSVVLPDSASVRGYARAMGFPLGNGLLEPA
                     TLQRLNAVVLGQDESRPSAWRTHPSHCETFDSEGHATGRVIQILPSRLIEEKIADLVT
                     WFELEMRERARHPLIAIAAFYLGFLAISPFELGNGRTIRVLVGLLMRRAGYDYVPYAS
                     LERHMEDLREDYYEALDRTQSGIWTGDADFSPWLDYFMELLDRHRRKLEKKIELERGS
                     SLMTPLQRTIVDTVREHGTVDARLLLQATGANRNTLKDNVRRLVTDGWLEKTGERRAT
                     KYRLAAHDRARDALGSESA"
     gene            complement(1726..1998)
                     /locus_tag="VD788_14605"
     CDS             complement(1726..1998)
                     /locus_tag="VD788_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77541.1"
                     /translation="MPCLVGCIALGAPRFAILLVVIFSDYLGRAYDTTIWPLLGFFFM
                     PLTTLAYAWAIHSRGSVEGLPLLVVVIAVLLDLGLLGGSSRRKRSG"
     gene            complement(2011..>3145)
                     /locus_tag="VD788_14610"
     CDS             complement(2011..>3145)
                     /locus_tag="VD788_14610"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HXV77542.1"
                     /translation="LIAWNLNLNTRDRKLANRIAAELRETGKLEREDGKIVRDADGNA
                     LRVPGRFTELQGGGWYIAEYGRAQISFNLLNYEVTSLHDVFDAACEEADRVGARITGS
                     ELVGLVPLDALLAAGDHYLARQGLTSGVPDRERIHAAVLSLGLGELAPFDPLEKVVEY
                     RYRGRPCGLKAMSLVEFTDELSSESPAPGGGSVAALCGSLSAALSSMVAALTWAKTGM
                     EDVRPEMLEIGRRAQALKAWFVDAVDRDTDAFNAVLAARRLAKRTAQEQRTRDAASER
                     ANQAATRVPLSVLDKAVEALELARAVARSGNPASVSDAGVGGACALAAAEGASLNVAI
                     NLPSIGDKAVAERMRAEQERLIARARELAAEVRGTVDGVLAES"
BASE COUNT          520 a         1112 c         1036 g          477 t
ORIGIN      
        1 cagcccgtcc aacacgtgag gatcggtgtc gtatcgcagg agcttccgct cgcgcgggcc
       61 gcagcgcgag gccaacgcgg tccggtgcgc cgtggtgatc aacgcgagcg ccgcccggat
      121 ctcgtcgatc gaagcgttct cgtcgatcgc ctcgatcgcc gccggcgcca tgccctcgac
      181 gacacgcgac agcaggcgct cgagcgcctc ccgttggtcg ccgtcgagac ggtcgaactg
      241 cacgccgagc cgcacggtgc gcgagtcgtc gagacggtgg gcgtgcgcga cccgccccga
      301 cagccgcagc tcgatgtcgc cggccgccga gcacagcgtc agaccgatct cgggtgcctc
      361 cgggatcggg atttcgcctt cgagcagcgt gcccgcgtac gacagatcgt gtgccgagca
      421 ggggaagctc ccgccgccgc agagcaacgc ggcagggacc gccgcgagga atcgcgacgc
      481 gcggtggtcc gaaggcgtcg tctcgtccga gggcatctgc gtcaaagtat aggcccgacc
      541 attgggtggg caagcggacc acgaagctcg cgagtggggg gttgccacgg gacggaatgg
      601 gtaataggat gacgacgccc aaagtcgcgt cactttatgg gtgtttttgg atgaggtttt
      661 aggtgtcact tcaattttcc ctccccgtca ccgagaccat cctgtccgtg atctcccagg
      721 ccgaccagat gcggggaaaa tggtccgccg cgattccgat cggcgaaggc cggctccagc
      781 gcattcgcga agcgaccgcc atccgatcga tcggcagctc gtgccggctg tcgggaatcg
      841 gcatctccga ccacgacgtc gctgggatcc tgcgcgggca gtcggtcgtg ctgccggaca
      901 gcgcgtccgt ccgcggctac gcacgggcga tgggcttccc gctgggaaac ggcctgctcg
      961 aaccggcgac gctgcagcga ctcaacgccg tcgtgctggg ccaggacgag tcgagaccga
     1021 gtgcgtggcg cacgcacccc tcgcattgcg agaccttcga ttccgaaggc catgcgaccg
     1081 gccgtgtgat ccagatcctg ccgtcgcgtc tgatcgagga gaagatcgcc gatctcgtca
     1141 cctggttcga gctcgagatg cgcgagcggg cgcgccaccc gttgatcgcg atcgccgcct
     1201 tctacctcgg gttcctcgcg atcagcccgt tcgagctggg caacggccgc acgatccgcg
     1261 tgctcgtcgg attgttgatg cggcgcgccg ggtacgacta cgttccgtac gccagcctcg
     1321 agcggcacat ggaagacctg cgtgaggact actacgaggc gctcgaccgc acgcagtccg
     1381 gcatctggac cggcgacgcc gacttctccc cgtggctcga ctacttcatg gaactgctcg
     1441 accgtcaccg gcggaagctc gagaagaaga tcgagctcga gcgcggttcg agtctgatga
     1501 ccccgctcca acgtacgatc gtcgacaccg tgcgcgagca cggcacggtc gatgcgcgtc
     1561 tgctcctgca ggcgaccggc gccaaccgca acacgctcaa ggacaacgtt cggcggctcg
     1621 tcaccgatgg gtggctcgag aagaccggcg agcgccgcgc aaccaagtac cggctggcgg
     1681 cccacgacag ggcccgcgac gccctcggct ccgagtcggc ttgagtcagc cggaccgttt
     1741 ccggcgcgag ctaccaccca gcaaacccag gtcgagcaac accgcgatga ccaccaccaa
     1801 cagcggcagc ccttcgaccg agcccctgct gtggatcgcc caggcgtacg ccagcgtcgt
     1861 cagcggcatg aagaagaagc cgagcagcgg ccaaatcgtc gtgtcgtagg cccggccgag
     1921 gtagtcgctg aagatcacga cgagcaggat cgcgaaacgc ggcgccccga gcgcgatgca
     1981 accgaccaga cacggcatcg ccctaccgcc tcacgattcg gcgagcacgc cgtcgaccgt
     2041 gccgcgcacc tcggcggcca actcccgagc gcgtgcgatc agccgttcct gctccgcccg
     2101 catgcgctcg gcgaccgcct tgtcgccgat cgacgggagg ttgatcgcga cgttcaacga
     2161 cgcgccttct gcggcggcca gcgcgcacgc gccgccgacg cccgcgtcgc tgaccgaagc
     2221 cggattgccg ctgcgcgcga cggcccgcgc caactccaac gcttcgaccg ccttgtcgag
     2281 gacggacaac ggcacgcggg tggcggcctg gttcgctcgc tcgctcgccg cgtcacgggt
     2341 gcgctgctcc tgcgccgtgc gtttggcaag gcggcgggcc gcgagaacgg cattgaacgc
     2401 gtcggtatcg cggtcgactg cgtcgacgaa ccacgccttc aacgcttgcg ctcggcgtcc
     2461 gatctcgagc atctccggcc gcacgtcctc cataccggtc ttggcccagg tcagcgcggc
     2521 gaccatgctc gacaacgcgg ccgagagcga accgcacagc gcggcgacgc tgccacctcc
     2581 cggggccggg gactcgctcg acagctcgtc ggtgaactcg acgagcgaca tcgccttgag
     2641 cccgcacggc ctgccgcggt agcgatactc gacgactttc tccagcgggt cgaacggggc
     2701 gagctcgccg agacccagcg acagcacggc ggcgtggatc cgttcgcggt ccggaacacc
     2761 gctggtcaaa ccctgccgcg ccagatagtg atccccggcc gcgagcagcg cgtcgagcgg
     2821 caccaagccg accagctcgc tgccggtgat cctcgcgccg actctgtcgg cttcctcgca
     2881 ggcggcgtcg aacacgtcgt ggagcgaggt cacctcgtag ttcagcagat tgaacgagat
     2941 ctgggcccgg ccgtactccg cgatgtacca gccgccgccc tgcagctcgg tgaaacgtcc
     3001 gggcacgcgc agcgcattgc cgtcggcgtc acgcacgatc ttcccgtcct cgcgctcgag
     3061 cttgcccgtc tcgcgaagct ctgccgcgat ccggttggcc agcttgcgat cgcgggtgtt
     3121 caggttcaga ttccaggcga tcagg
//
LOCUS       DASPGN010000505         6917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_444798, whole genome shotgun sequence.
ACCESSION   DASPGN010000505 DASPGN010000000
VERSION     DASPGN010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444798"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="VD788_14615"
     CDS             <1..1607
                     /locus_tag="VD788_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77543.1"
                     /translation="SISDDGSAIVARIDEALYWRRLDEFDLRPVPRSSRSTGWVVSPD
                     GDGLLFHVSMDEIIRRGELRRWSAENGNTVTIRETDRMLAPLEWGERGLVARAGQTSI
                     ARFETLDTPVDRMERLFETTTWTRRVETAVRVPDSDRWIVGIFPVGGERPELVIATPG
                     DDESPLLADAARPRIAPGGRLLFLQDDTLMAIAFDRSALRVAGDRIPLIAGVVDYDLS
                     RDGTLVYLPSSGERTARRVVRVERDGSTSVLVAGGHNYVHARLSPDGRYVSTVDPQLR
                     LHEIATGSTRLLEFAAAGATGADHVWTPDGRFVVYTAFLVDGDKRRCVLYRQAVDGRT
                     PPEALIEPEGDDEVYPTDYAPDGSGLVYALYRDQTDLWFQPADGSPPRAILATATSED
                     LGRFSPDMRWLAYESNEGGTQEVWIAAYSKADRRLGAKWRVSSGGKDPIWSPRGDELF
                     YEDLDGRLIAVALSAGDGGVALGESSALFDLRELRIRSSGDQSYDVDPSGRSFIMVTS
                     DRPEVREVRMVSGWLAELDRLAPPAR"
     gene            1670..2707
                     /locus_tag="VD788_14620"
     CDS             1670..2707
                     /locus_tag="VD788_14620"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXV77544.1"
                     /translation="MGTGGSRWTPWRVGIGAVLLLVVLGLLYQRFATRPPPAPRVVTI
                     YAFSIMQEVLEEALLPAFRERFSRATGERVEFAVTYGGSAWITDEVLRRVGAEIVILS
                     SEIDAYRLVEGGLLGGAIWRHLPSGGIISASPIVLAVREGNPLDLRDFADLARPGVEV
                     LHADPLTSGAGEWSVLAIHATTLPAAGGGVVSRPVALTDLLPNVIGMPSSAREMRREF
                     ERGVGDVMITYEIEARATAGAEPVYPARTMLSRHVVCKIREHIDPERGAVIDALLEFL
                     FTQEAQAELTRHGFRPGDVVSDGERPETRWLTLDDLGGARKARREVLDPLWREGIFSG
                     ETGRESSERGR"
     gene            2712..3443
                     /locus_tag="VD788_14625"
     CDS             2712..3443
                     /locus_tag="VD788_14625"
                     /inference="COORDINATES: protein motif:HMM:NF022142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2490 domain-containing protein"
                     /protein_id="HXV77545.1"
                     /translation="MRPDSNRSAARTRRTGSILVGLLSGLCLSPVAAQVDDDQLGAWY
                     MYFWSTRLDDGRFGFQGDGQYRAWDLGGDLEQLLLRGGATYTPRSGGVTFTLGYANVT
                     SGEFGGDDDTSGESRIYQEALLPQKVGPRVHLGHRFRFEQRWVENQDFRTRFRYALFV
                     TIPVNDDRLRQGTISVVLSDELFINGQREIGDGREVELFDRNRLYGAIGYSFSDALRV
                     QGGYMYQNTDEFGKGQLQLSLHHAF"
     gene            3433..3633
                     /locus_tag="VD788_14630"
     CDS             3433..3633
                     /locus_tag="VD788_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77546.1"
                     /translation="MRSDRDWHRTALALVVTTMAYNVVEAGVALWRLALEARGSYGDA
                     SNGRRRSCTASSAPPSSCSRCT"
     gene            3585..3962
                     /locus_tag="VD788_14635"
     CDS             3585..3962
                     /locus_tag="VD788_14635"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HXV77547.1"
                     /translation="MHRVVGATFLVLALYVTVQAGWTLWARAAPEESLAGIVLAVASL
                     VVMPLVAWGKLRAAREIGSAALRAEAKETLACSYLSLALVLGLAVNAAAGWWWADPVA
                     ALLMVPWLVNEGQEGLRGAPCAC"
     gene            4020..5192
                     /locus_tag="VD788_14640"
     CDS             4020..5192
                     /locus_tag="VD788_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXV77548.1"
                     /translation="MPPLLSALVVVAMPAVAAPGDAGGEAVDLAALATQLVRDYHAGD
                     ADALRPHVAPGMVFRDPGLGQRFDLDGFVASVAATAGARTLAIEWTGVAGSRADVRGR
                     WTVPGDGGGRPRELSFSIELEFDPTGERPRVVSWLDDFRRGPMWKPARGNAGAQTEHF
                     TVVYFDDELSAEEANRLGATLEKWYDATRRYLGRSFDEGYRLDVDVAGAHGSPFASNP
                     GPEAFILVPARSAKREYAFSMVHELTHNLVGLSWLCRNEHERNGVELPAGNRLLDEGL
                     GVYVEEKLTGEGPRVFPNFGEETHAAYWRAREAAGEPVWPALEAELHREHGDRRLGYL
                     AQASFCKFLVDTYGLDRFLRLFAVDPAAAESIYDKPFAALDDEWRRFLGERFGAAQ"
     gene            complement(5186..6433)
                     /locus_tag="VD788_14645"
     CDS             complement(5186..6433)
                     /locus_tag="VD788_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HXV77549.1"
                     /translation="MSGRAAAIWVSLLAFGAGAAATLPAEWEHWRYWRTIDAATGSSL
                     VAAVLPPAVHGQARPSLADLRLIDGRGDELPYVLFVDTGERTVDWRSAELSEAGFVDG
                     RYSQVVADGGSGDRVHDTLRVDVVEDDFFTWVAVEASDDRASWRLVRERAPIYRFKRD
                     GLEGKNEIGYPPTRSRWLRLRLLAQQELTVRAVRIAETTAREPELEAWAAAARREADP
                     PAGESRFRIDLGHEHVPVSAVRFATAAAEFHRPVVVSVRGESGDWRQVAEGDVYRYRA
                     GGGDAASRERLEFRFAEDRGREWRVAIFDRSDAPIDDLRLELVGTPRRVVFRPESAPP
                     FRLLYGNDRAEPPSYELARVTVREDWERASTVPLSDESENEAWVSPEPWTERHPLVLW
                     AALGLAILVLGGLALRALGAPRH"
     gene            complement(6430..>6917)
                     /locus_tag="VD788_14650"
     CDS             complement(6430..>6917)
                     /locus_tag="VD788_14650"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HXV77550.1"
                     /translation="SRVLLNARFATFAVATFAFASAFLLARSRRPALRAAERSVFAVV
                     GAAVNVLAVWALSLEVWDFFALVELGVDRSLARQMGLSILWALSATTLIVSGVRRRSA
                     GLRWQGLALLAVAICKVFVFDLSFLERVYRVLSFLALGLLLLIVSFLYQRRPAGPGSG
                     S"
BASE COUNT          996 a         2464 c         2493 g          964 t
ORIGIN      
        1 tgtcgatctc ggacgacggc tcggcgatcg tcgcccggat cgacgaagcg ctgtactgga
       61 ggagactcga cgagttcgat ctgcggccgg ttccgcggtc gtcgcgatcg acgggatggg
      121 tcgtctcgcc ggacggcgac gggctgctgt tccacgtgag catggacgag atcatccgtc
      181 gcggcgagct gcgtcgctgg tcggccgaga acggcaacac ggtcacgatc cgcgagaccg
      241 accggatgct cgcgccgctc gagtgggggg agcgagggct ggtcgcgcgt gccggtcaga
      301 cgagcatcgc ccggttcgag accctcgaca cgccggtcga tcggatggag cggttgttcg
      361 agaccaccac ctggacgcgg cgggtggaga cggccgtgcg cgtgccggac agcgaccgtt
      421 ggatcgtcgg gatcttcccg gtcggcggcg agcggccgga gctggtgatc gcgacgcccg
      481 gcgacgacga gagcccgttg ctcgccgacg ccgcgcgacc ccgcatcgcg cccggcgggc
      541 gcttgctctt cttacaggac gacacgctga tggcgatcgc cttcgaccgg agcgcgttgc
      601 gcgtggcggg ggaccgcatc ccgttgatcg ccggcgtcgt cgactacgac ctgtcgcgcg
      661 acgggacgct cgtgtacctg ccgtcgtcgg gcgagcgaac ggcacgccgg gtcgtgcggg
      721 tcgaacgaga cggttcgacg tcggtcctgg tcgccggcgg gcacaactac gttcacgcgc
      781 gtctgtcgcc ggacggccgg tacgtgagca cggtcgaccc gcagttgcgc ctgcacgaga
      841 tcgcgaccgg gtcgacccgg ctgctcgagt tcgccgcggc gggtgcgacc ggcgccgacc
      901 acgtctggac ccccgacggc cgcttcgtcg tctacaccgc gttcctggtc gacggcgaca
      961 agcggcggtg cgttctctac cggcaggcgg tcgacggtcg gacaccgccc gaggcgttga
     1021 tcgagccgga aggcgacgac gaggtctatc cgaccgacta cgcgccggac ggcagcggcc
     1081 tcgtctacgc gctgtaccgc gaccagaccg acctgtggtt ccagccggcc gacgggtcgc
     1141 cgccgagggc gatcctggcg accgcgacct cggaggacct cggccgattc tcgcccgaca
     1201 tgcgctggct ggcgtacgaa tcgaacgagg gcgggacgca ggaggtatgg atcgcggcct
     1261 actcgaaggc cgaccgccgg ctgggcgcga agtggcgcgt gtcgagcggc ggcaaggacc
     1321 cgatctggtc gccgcgcggc gacgagctgt tctacgagga tctcgacggt cgtctgatcg
     1381 cggtcgcgct gtcggccggc gacggcggcg tggcgctcgg cgagtcgagc gcgctgttcg
     1441 acctgcgcga gctgcggatc cgctcgagcg gggaccagtc ctacgacgtc gatccgtcgg
     1501 ggaggtcctt catcatggtg acgtcggacc ggccggaggt gcgcgaggtg cggatggtct
     1561 cgggctggct ggccgagctc gaccggctgg cgccgcccgc gcgctgagga gcgagcccgt
     1621 ccgatgacga gcctcggcga ggactacaat cccgtcggag ggtgcgatca tgggcaccgg
     1681 cggaagccgt tggacgccgt ggcgtgtcgg catcggggcg gtgctgctgc tcgtcgtcct
     1741 gggcctgctc tatcagcgct tcgcgacgag accgccgccc gccccgcgcg tcgtcacgat
     1801 ctatgcgttc agcatcatgc aggaggtgct ggaggaggcg ctgctcccgg cgttccgcga
     1861 acgcttcagc cgcgccaccg gcgagcgcgt cgagttcgcc gtcacctacg gcgggtcggc
     1921 ctggatcacg gacgaggtgc tgcgtcgcgt cggcgccgag atcgtcatcc tgtcgtccga
     1981 gatcgacgcc taccgcctgg tcgagggcgg cttgctcggc ggcgcgatct ggcgccacct
     2041 gccgtcgggc gggatcatca gcgcctcgcc gatcgtgctg gcggtgcgcg agggcaaccc
     2101 gctcgacctc cgtgatttcg ccgacctcgc ccggccgggc gtcgaagtgc tgcacgccga
     2161 cccgctgacc tcgggcgccg gcgagtggtc ggtgctcgcg atccacgcga cgacgctgcc
     2221 ggcggcgggc ggcggcgtgg tcagtcgacc cgtcgcgctg accgacctcc tgccgaacgt
     2281 gatcggcatg ccgtcctccg cgcgcgagat gcgccgagag ttcgagcgcg gggtgggcga
     2341 cgtgatgatc acctacgaga tcgaagcgcg ggcgaccgcc ggcgccgagc cggtctaccc
     2401 cgcgcggacg atgctcagcc ggcacgtcgt ctgcaagatc cgcgagcaca tcgaccccga
     2461 gcgcggcgcc gtgatcgacg ccctgctcga gttcctgttc acgcaggaag cccaggcgga
     2521 gctgacgcgc cacggcttcc ggcccggcga cgtcgtctcg gacggggagc ggccggagac
     2581 gcgctggctc acgctcgacg atctgggcgg cgcgcgcaag gcgagacggg aggtgctcga
     2641 cccgttgtgg cgggagggca tcttttcggg ggagacaggc cgcgagtcca gcgagcgcgg
     2701 gagatgagtc ggtgagaccg gattccaacc gatcggcggc gcggacccga cgaaccggtt
     2761 cgatcctcgt cgggttgctg tcaggtctgt gcctgagccc cgtcgccgcg caggtcgacg
     2821 acgatcagct gggcgcgtgg tacatgtatt tctggagcac gcggctcgac gacggccgct
     2881 tcggcttcca gggcgacggc cagtaccgcg cgtgggatct cggtggcgac ctcgagcagc
     2941 tgctgctgcg cggcggcgcg acctacacgc cgcggtcggg cggcgtgacc ttcacgctgg
     3001 gctacgccaa cgtcacctcg ggcgagttcg gcggcgacga cgacaccagc ggcgagagcc
     3061 ggatctacca ggaagcgctg ctgccgcaga aggtcgggcc gcgtgtccac ctcggccacc
     3121 gctttcgctt cgagcagcgc tgggtcgaga accaggactt ccgcacgcgc ttccgctacg
     3181 ccttgttcgt cacgatcccc gtcaacgacg accggctgcg ccagggcacg atctcggtcg
     3241 tgctctccga cgagctgttc atcaacggtc agcgggagat cggcgacggc cgcgaggtcg
     3301 agctgttcga ccgcaaccgg ctgtacggcg cgatcggcta ctcgttctcc gacgcgttgc
     3361 gcgtccaggg cggctacatg taccaaaaca cggacgagtt cggcaagggt cagttgcagc
     3421 tcagtctgca ccatgcgttc tgaccgagac tggcaccgca ctgcgctcgc gctcgtcgtc
     3481 acgacgatgg cctacaacgt cgtcgaggcc ggggtggcgc tgtggcgcct ggcgctcgag
     3541 gcgcgcggct cgtacggcga cgcgtcgaac gggcggagac gatcgtgcac cgcgtcgtcg
     3601 gcgccacctt cctcgtgctc gcgctgtacg tgaccgtgca ggccggctgg acgctgtggg
     3661 cccgcgccgc gcccgaggag agcctcgccg gcatcgtgct cgccgtcgcg tcgctcgtcg
     3721 tcatgccgct cgtcgcgtgg ggcaagctgc gtgccgcccg cgagatcggc agcgccgcgc
     3781 tgcgcgccga ggccaaggag acgctggctt gctcctacct gtcgctcgcg ctcgtcctcg
     3841 gcctcgcggt gaacgcggcg gccggctggt ggtgggccga tcccgtcgcc gccttgctga
     3901 tggtgccgtg gctcgtcaac gaaggacaag aagggttgcg cggcgcgccg tgcgcctgct
     3961 agcccgtcac cgactatact cccgatccga tgcaccgccg cacgacaggc cgagccaccg
     4021 tgcccccgct cttgtcggcg ctcgtggtcg tcgcgatgcc ggcggtcgcg gcgcccggcg
     4081 atgccggcgg agaggcggtc gacctggccg cgctcgccac gcagttggtg cgggactacc
     4141 acgccggcga cgccgacgcg ttgcgcccgc acgtcgcgcc cgggatggtc tttcgcgacc
     4201 ccggtctcgg ccagcggttc gacctcgacg gcttcgtcgc gtcggtcgcg gcgacggccg
     4261 gtgcccgcac gctcgcgatc gaatggaccg gcgtcgccgg cagccgcgcc gacgtgcgcg
     4321 gcaggtggac ggtgcccggc gacgggggcg gtcgtccgcg cgagctgtcg ttctcgatcg
     4381 agctcgagtt cgacccgacg ggcgagcgcc cgcgcgtcgt ctcgtggctc gacgacttcc
     4441 ggcgcggccc gatgtggaag ccggcgcgcg gaaacgccgg tgcccagacc gagcacttca
     4501 ccgtcgtcta cttcgacgac gagctgtcgg ccgaggaggc gaaccgactc ggcgcgacgc
     4561 tcgaaaagtg gtacgacgcc acccgacgtt atctcggtcg ctcgttcgac gagggctacc
     4621 gtctcgacgt cgacgtcgcc ggtgcccacg gcagcccgtt cgcgtcgaac ccaggtcccg
     4681 aggccttcat cctcgtaccc gcgcgctcgg ccaaacgcga gtacgccttc tcgatggtcc
     4741 acgagctgac gcacaacctg gtcggcctga gctggctgtg ccgcaacgag cacgagcgca
     4801 acggggtcga gctgccggcg ggcaatcgcc tgctcgacga gggcctcggc gtttacgtcg
     4861 aggaaaagct gaccggcgaa gggccgcgcg tctttcccaa cttcggcgag gaaacgcacg
     4921 ccgcctactg gcgcgcacgc gaggcggccg gcgagccggt ctggccggcg ctcgaagccg
     4981 agctccaccg cgagcacggc gatcgacgac tcggctacct ggcccaggcc tcgttctgca
     5041 agttcctcgt cgacacctac ggactcgatc gcttcctgcg gctgttcgcc gtcgatccgg
     5101 ccgccgccga gtcgatctat gacaagccgt tcgccgcgct cgacgacgag tggcggcggt
     5161 ttctcggaga gcgattcggc gcggctcagt gacggggcgc gccgagggca cgcagcgcga
     5221 gcccgccgag cacgagaatc gccaacccga gcgcggccca cagcacgagc gggtgccgct
     5281 cggtccacgg ctcggggctg acccacgcct cgttctccga ctcgtccgac agcgggacgg
     5341 tcgaggcgcg ctcccaatcc tcgcgcaccg tcacgcgcgc cagctcgtag gacggcggct
     5401 cggcgcgatc gttgccgtac aacagccgga agggtggcgc ggactcgggc cgaaagacga
     5461 cgcggcgcgg cgtgccgacg agctccaacc gcagatcgtc gatcggcgcg tcgctgcggt
     5521 cgaagatcgc gacgcgccac tcgcgaccgc ggtcctcggc gaagcggaac tcgagccgct
     5581 cgcgcgaggc cgcatcgccg ccgccggcgc ggtagcggta gacgtcgcct tcggcgacct
     5641 ggcgccagtc acccgactcc ccgcgcacgc tgaccacgac cgggcgatgg aactccgccg
     5701 ccgcggtcgc gaagcgaacc gccgacaccg gtacgtgctc gtgaccgagg tcgatccgga
     5761 agcggctttc gccggccggc gggtccgcct cccgccgcgc cgcggccgcc cacgcttcca
     5821 gctccggctc gcgcgccgtc gtctcggcga tgcgcacggc gcgcaccgtc agctcctgtt
     5881 gcgcgagcag ccgcagccgc agccaacgcg accgcgtcgg cgggtagccg atctcgttct
     5941 tgccctcgag gccgtcgcgt ttgaagcggt agatcggcgc ccgctcgcga accaggcgcc
     6001 acgaggctcg atcgtcgctc gcctcgacgg cgacccaggt gaagaagtcg tcctcgacga
     6061 cgtcgacgcg caacgtgtcg tgcacccggt cgccgctgcc gccgtccgcc acgacctgcg
     6121 agtagcggcc gtcgacgaag ccggcctcgc tcagctccgc cgaccgccag tcgaccgttc
     6181 gctcgccggt gtcgacgaac agcacgtaag gcagctcgtc gccccgcccg tcgatcagcc
     6241 gcaggtcggc gagcgacggc cgcgcctggc cgtggaccgc aggcggcagc acggccgcga
     6301 cgagtgagga gcccgtggcg gcgtcgatcg tccgccagta gcgccagtgc tcccactccg
     6361 ccggcagcgt cgccgcggcc cccgcgccga acgcgagcag cgagacccag atcgcggcgg
     6421 cccgcccgct cacgagccgg accccggacc ggccggccgg cgctggtaca ggaacgacac
     6481 gatcagcaac aacaacccga gcgcgaggaa cgacagcacg cgatagacgc gctcgaggaa
     6541 cgacaggtcg aagacgaaca ccttgcagat cgcgaccgcg agcagcgcca gcccctgcca
     6601 gcgcagcccg gccgatcgcc gacgcacgcc cgagacgatc agcgtcgtcg ccgacagcgc
     6661 ccacaagatc gacagcccca tctggcgcgc gaggcttcga tcgacgccga gttcgacgag
     6721 cgcgaagaaa tcccagacct cgagcgagag cgcccagacc gcgagcacgt tgaccgccgc
     6781 gccgacgacc gcgaacaccg agcgctcggc ggcgcgcagc gccggccggc gagagcgggc
     6841 gagcaggaac gccgaagcga acgcgaaggt cgcgacggcg aacgtggcga agcgcgcgtt
     6901 gagcagcacg cgcgacg
//
LOCUS       DASPGN010000506         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_328971, whole genome shotgun sequence.
ACCESSION   DASPGN010000506 DASPGN010000000
VERSION     DASPGN010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328971"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VD788_14655"
     CDS             <1..1028
                     /locus_tag="VD788_14655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HXV77551.1"
                     /translation="DDRTREAIARAIGVPLERDEDQVGRLRRLFDSRKRPFSAEQCKQ
                     ADKPAGADWIENELGSAPGIRCPVGRATVVALPGVPAEMQRMFDQSVVPWLADRVSGR
                     PARRTLKVGGRLESSVDRQLRDLYGVDGVDVTILAGSEGIELQLAARADSADEARRAI
                     ERLERRIRDRLGDDLYGVDDETPAEIVGRMLRERGLTVATAESCTAGLLAAAITEVPG
                     SSAWFRGGIVVYHDDTKERFLSIPRAMIDRHGAVSEEVGRALAAGVRREFAADVGLGV
                     TGIAGPGGGSPDKPVGLVQVALADADGIDSHETHQVGDRRLIRRRTVLYVLDLLRRRL
                     LARPGRP"
     gene            1025..1615
                     /gene="thpR"
                     /locus_tag="VD788_14660"
     CDS             1025..1615
                     /gene="thpR"
                     /locus_tag="VD788_14660"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXV77552.1"
                     /translation="MRLFLAVRPADHVVERLRELQRMLGATHPGWRWVPPSQIHLTLR
                     FLGEVDEATELAAQSHWQRAARSVPRFRLALGGVGRFPPRGRARVLWIGVGDPDGGTA
                     LAELAERIEQATRLSGFAAERRPFHSHLTLARAGKQAPGAVPIAADAVHAPPFLVDRV
                     VLFQSELGPTGARYTERSSFALAPPLAGSSDADAAV"
     gene            1612..>2396
                     /locus_tag="VD788_14665"
     CDS             1612..>2396
                     /locus_tag="VD788_14665"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXV77553.1"
                     /translation="MKPVQEQRPTRPGGGPVAIIGGGAWGTALSIQLARNGWAVRLWI
                     RESELVERVRARRDNPIYLPGVRIPHEVSAHDTLAGAVDGADLVLMTVPSQFARPIYH
                     SLGALLAPALPIVVACKGIEEGTLALPLEAAREELGSEHRLAILSGPSFAAEVAEGRP
                     TAIVVAASEPGLAGTVQRRFSSNSLRIYTNSDPLGVQLAGALKNVVAIAVGIAEALEM
                     GTNARAALITRGLNEMIRLGVALGGRPATFSGLAGLGDLVLTCT"
BASE COUNT          308 a          847 c          883 g          358 t
ORIGIN      
        1 aggacgaccg cactcgcgag gcgatcgcgc gggcgatcgg cgtgccgctc gagcgcgacg
       61 aggaccaggt cggccggctc cgccggttgt tcgacagccg caaacgcccg ttctccgccg
      121 aacaatgcaa gcaggccgac aagccggccg gtgccgactg gatcgaaaac gagctcggtt
      181 ccgcgcccgg tatccgctgc ccggtcggcc gtgcgacggt cgtcgcattg ccgggagtgc
      241 ccgccgagat gcagcgcatg ttcgaccagt cggtcgtccc gtggctcgcg gatcgggttt
      301 ccggccgccc cgcccggcgg acgctcaagg tcggcgggcg actcgagtca tccgtcgacc
      361 ggcagttgcg cgacctctac ggggtcgatg gcgtcgacgt gacgattctc gccgggagcg
      421 aagggatcga gctgcagctc gccgcgcgcg ccgactccgc cgacgaggct cgccgagcga
      481 tcgagcgcct cgagcggcgg atccgtgacc gcttgggcga cgacctgtac ggcgtggacg
      541 acgagacgcc ggccgagatc gtcggccgga tgttgcgcga gcggggtctg acggtcgcga
      601 ccgcggaatc ctgtacggcc ggactgctgg ccgcggcgat cacggaggtg ccgggctcgt
      661 cggcgtggtt ccgcggcggc atcgtggtct accacgacga cacgaaggag cgtttcctgt
      721 cgatcccgcg ggcgatgatc gaccgccacg gggcggtgag cgaggaggtc gggcgcgcac
      781 tggccgcggg cgtgcgtcgc gaattcgccg ccgacgtcgg tctcggtgtg accggaatcg
      841 ccggtcccgg tggcgggagc ccggacaagc cggtcggttt ggtccaggtc gcgttggccg
      901 acgcggacgg gatcgactcg cacgagaccc atcaggtcgg agaccggcgg ctgatccgtc
      961 gtcgcacggt gctgtacgtg ctcgacctgc tgcggcgcag gctgctcgcg cgccccgggc
     1021 gcccgtgaga ctgttcctcg ccgtccgtcc ggcggatcac gtcgtcgagc ggcttcgcga
     1081 gctgcaacgc atgctcggag cgacgcaccc gggctggcgc tgggtccctc cctcgcagat
     1141 ccacctgacg ctgcggtttc tcggcgaggt cgacgaggcg accgagctcg cggcacagtc
     1201 tcactggcag cgcgccgccc ggagcgtgcc ccgctttcgg ttggcgctcg gcggcgtcgg
     1261 ccgattcccg ccacgcggtc gtgcgcgcgt cctgtggatc ggcgtcggtg atcccgacgg
     1321 cggcacggcg ctcgccgagc tggccgagcg catcgagcag gccacccgcc tgtcgggatt
     1381 cgcggccgag cggcggccgt tccactcgca cctgacgctg gcgcgcgccg gcaagcaggc
     1441 gcccggcgcc gtcccgatcg cagcggatgc ggtccacgct ccgccgttcc tcgtcgaccg
     1501 ggtggtgctg ttccagagcg agctcggccc gaccggggcg cgctacactg agcgctcgtc
     1561 gttcgcgctc gctccgcctc tcgcggggag ctcggacgcc gatgcggcgg tgtgaaaccc
     1621 gtgcaggagc agcgacccac gcgaccgggt ggcggacccg tggcgatcat cggcggcggt
     1681 gcctggggca ccgccctgtc gattcaactc gcccgcaacg gctgggcggt ccggttgtgg
     1741 atccgcgaga gcgagctggt cgaacgcgtc cgcgcgcgtc gcgacaaccc gatctacctg
     1801 cccggcgtgc ggatcccgca cgaagtcagc gcgcacgaca ccctcgccgg cgcggtcgac
     1861 ggcgccgatc tcgtgttgat gaccgtaccg tcccagttcg cccggccgat ctaccactcg
     1921 cttggcgcgc tgctcgcgcc ggcgctgccg atcgtcgtgg cgtgcaaggg gatcgaggag
     1981 ggcacactcg cgctcccgct cgaggcggct cgcgaggagt tgggtagcga gcatcgtctg
     2041 gcgatcctct ccggtccgag ctttgccgcc gaggtcgccg aaggccgccc gaccgcgatc
     2101 gtcgtcgcgg cgagcgagcc ggggttggcc ggcaccgtcc agcggcgctt ctcgtcgaac
     2161 agcctgcgga tctacaccaa ctccgacccg ctgggcgtgc agctcgccgg cgcgctgaag
     2221 aacgtcgtcg ccatcgccgt cggcatcgcc gaggcgctcg agatgggaac caacgcccgt
     2281 gcggcgttga tcacccgggg tctgaacgag atgatccgac tcggtgtcgc tctcggcggc
     2341 cgaccggcga cgttcagcgg gctcgccggg ttgggcgatc tggtgctgac ctgcac
//
LOCUS       DASPGN010000507         6820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1029810, whole genome shotgun sequence.
ACCESSION   DASPGN010000507 DASPGN010000000
VERSION     DASPGN010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029810"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VD788_14670"
     CDS             <1..1206
                     /locus_tag="VD788_14670"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXV77554.1"
                     /translation="GASRAGPGWLQVRIGRLVDGIVDAAAHHRLVVLGGAGLLTAVAT
                     WGATRVEFDASPRALRPVDHPAAELERVLADDFSLGLDGFTVIVHGPSLDVALGRADE
                     AAVVLRDQLDRSAAVYSPADYLLSGALLDERIAAARRVIDESVPALLRDELRRRGFDL
                     VPFSPALEVLDAIAAGRPLPDTAADRRPDWLDELVRVEHGTVWATVQVAGQTGQWPDG
                     PPRRVLDALAERLPDATVASISLVGASLRRLVARELRVLSGWCFLIICAVVLVSFRLD
                     VRSSALSLLPVSCGGLWVIGACGLVGIPISLFSVTAAPLLLGLGIDDGLHALHGIRFH
                     GSLRRSLHSVGPAITITTLSTCLGFGSLMLSRLPALRSGGLVIALGTGLCLVATIVLL
                     PAVWRDPAE"
     gene            1212..2198
                     /locus_tag="VD788_14675"
     CDS             1212..2198
                     /locus_tag="VD788_14675"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV77555.1"
                     /translation="MRSSGAEAPHAPTTGRLSRLLGPFHVTGPFWYRLHLFGMKILPW
                     WMVGLVIHLFTAFFFCTLIRIRRAIASNLDAAIGPADWWERQRRIHRTMLALAWSLSE
                     RYEWLAGKTRTDAEVDNLAAWRAVAGTDRGLIVVTAHVGNWEIGSALPARRERRRIHL
                     VREEELDPRAQRFIRQLLSRQTDDRYTTHFATGDPRLGILLREALERGDVVALQGDRP
                     RAGGKVLEVSLFGRPFRVPVGPFALARVAEVPLLPVFMLREGRTRYRVHFRQPIRVAR
                     SSDRERDHRSAAERFAAEIEWAISAAPHQWFCFNELWPGSPTGRPARSRRTC"
     gene            complement(2161..2598)
                     /locus_tag="VD788_14680"
     CDS             complement(2161..2598)
                     /locus_tag="VD788_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77556.1"
                     /translation="MTPLPPDPAALLPHDGVARFVDRVLELDADRIVCAGRIPEGSPF
                     AVAGRAPSFLAIELAAQAAALLECARREGPSRRARLGYLVRVRRAEFGGPFLAVEQTL
                     RVHVRRSAAAPPLYRYDAEVESAGERCFAGSISTFVETERAAR"
     gene            complement(2595..2948)
                     /locus_tag="VD788_14685"
     CDS             complement(2595..2948)
                     /locus_tag="VD788_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77557.1"
                     /translation="MSAFELLDDGSTAPERVVSVRVPADCPYFSGHFPHRPILPAIGQ
                     LALVADLLERAEPGAAWIAGAEALRFVRPILPGDELTVRIEPALAGEATAFRIERAGR
                     LVSEGRLRRRRTERR"
     gene            3117..3533
                     /locus_tag="VD788_14690"
     CDS             3117..3533
                     /locus_tag="VD788_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77558.1"
                     /translation="MADQVKKLSYCYLTLPHRAGQGDRVLGDLERAGVNLLAFSAFPD
                     KGGKAQIDLAADNLAPLRKIAKQNGWRLSAAKKGFLVQGEDRVGAVRRHIRKLAQEKI
                     NITAADAVAAGKGRYGMILWVKSKDYRRAAQVLRAK"
     gene            3540..4427
                     /locus_tag="VD788_14695"
     CDS             3540..4427
                     /locus_tag="VD788_14695"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXV77559.1"
                     /translation="MARTKAGDYSPQVLRLFDRFVHGEISRREFLDRAASLVVAGVSA
                     AALLDALTPDFAAGQLIAADDSRLATSRPEYDSPRGGGKMRGYLARGAAGPERLPGVL
                     VIHENRGLNPHIEDIARRLAVEGYLAFAPDALAPLGGYPGDEDKARELFRELDRDKLV
                     EDFVAAFEFLDAHPGCTGKVGAVGFCFGGGMVNRLAVRLPKLDAGVAFYGGQVPADQA
                     AKIEAPLLLHYAGIDDRINAGWPAYKAALESAGVRFEAFVYEGVHHGFNNDTTPRFDE
                     EAAGLAWRRTLEFFDKYLR"
     gene            complement(4433..4843)
                     /locus_tag="VD788_14700"
     CDS             complement(4433..4843)
                     /locus_tag="VD788_14700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HXV77560.1"
                     /translation="MPAERILFLTVDEGLAMHERLVERFGGLSGVRDAGLLESALYRP
                     QTGYYRDLADMGTALFESLLMNHPFVDGNKRVAFFATDVFFRLNGWKLAVDAGRTHRF
                     LIRLLDGGTCDFDHLRPWVRRSLARSSRRPSVDR"
     gene            complement(4833..5045)
                     /locus_tag="VD788_14705"
     CDS             complement(4833..5045)
                     /locus_tag="VD788_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77561.1"
                     /translation="MAAIKVSSKVESSVWEEFKELAEESHQSLSGLLTEAIREYLQRR
                     RVRPIVQRHLEDSIVENEKLGRLLAR"
     gene            5365..>6820
                     /locus_tag="VD788_14710"
     CDS             5365..>6820
                     /locus_tag="VD788_14710"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXV77562.1"
                     /translation="MRRALLSSLILLSPTWAFASNTGPVDLDAAARRFATASFPELYA
                     LLSLPNDAHFPRDIEKNVAFCEAAFAKRGFSTRRLTTPGAPLLLAERTFDRGEKTVLI
                     YLQIDGQPVDPDAWDQESPWTPVLKERDASGAWRIIPADRLEGGTPNPEWRIFARAAS
                     DAKGPVAAFLASLDAAAALEVAPDFDIKVIMDFEEELGSPNLPSAVERFRSELAADRL
                     VIFDGPRHISNRPTLTYGARGIAEMTLVVYGPRTPVHSGNYGNYTPNPAMRLARLLAS
                     MKDDRGRVTIPGFYDGVQLTQAEKETLRQVPDDEGEIRKFLGIAEPDAIGDNFQESLQ
                     YPTLNIRGLDALHVGNEARTLIPDRAVAEIDIRLVPSSDAPRLIALVTEHVARQGYLV
                     LDRAPDEEERMTHARIASIRSSVSYGPFQTPFESPTGNWLRRAMTRAFGHEPIQIRMM
                     GGSIPIAPFVTTLGIPAVGVPTVNPDNNQHAENEN"
BASE COUNT         1018 a         2402 c         2342 g         1058 t
ORIGIN      
        1 ggcgcgagtc gagccggccc cgggtggctt caggtccgca tcgggcggct ggtcgacggc
       61 atcgtcgacg cggcggcgca tcatcggctc gtcgtgctcg gcggcgcggg cttgctcacc
      121 gccgtcgcga cgtggggcgc cacgcgggtc gagttcgacg cgagccctcg cgcgctgcgt
      181 cccgtcgatc atccggccgc cgagctcgag cgcgttctcg cagacgattt ctccctgggt
      241 ctcgacgggt tcacggtgat cgtgcacggg ccgtcgctcg atgtcgccct gggccgagcc
      301 gacgaggccg cggtcgtcct gcgcgaccag ctcgaccggt cggcggcggt ctattctccg
      361 gccgactacc tgctctcggg tgcgctgctc gacgagcgta tcgcggccgc tcgccgcgtg
      421 atcgacgaga gcgtgcccgc gctcctgcgc gacgagctcc gccgccgcgg cttcgacctc
      481 gtgccgttct cgccggcgct cgaggtgctc gatgcgatcg ccgcgggccg cccgcttccc
      541 gacacggccg ccgatcgtcg gccggattgg ctcgacgagt tggtccgggt cgagcacggc
      601 accgtctggg ccaccgtgca ggtcgcggga cagaccgggc agtggcccga cggcccgccg
      661 cgccgggtgc tggacgcgct ggcggagcgg ttgccggatg cgacggtggc ctcgatcagc
      721 ctggtcggcg cgagcctccg ccgtctcgtc gcccgcgagc tgcgtgtgct ctccggctgg
      781 tgcttcctga tcatctgcgc ggtcgtgctg gtctcgttcc ggctcgacgt tcgttccagc
      841 gcgctttcgc tcttgccggt cagctgcggt ggtctgtggg tcatcggggc atgcggcctg
      901 gtcgggatcc cgatcagctt gttctcggtc accgccgccc cgctcctgct cgggctcggc
      961 atcgacgacg gcctgcacgc gctgcacggc atccgcttcc acgggagcct tcgccgttcg
     1021 ctgcacagcg tcggtccggc gatcacgatc acgacactgt cgacctgcct cggtttcggt
     1081 agcctgatgc tgagccgtct gccggcgctg cgcagcggag gactggtgat cgcgctcgga
     1141 acggggttgt gcctggtggc gacgatcgtc ctgcttccgg ccgtctggcg cgatccggcg
     1201 gagtagcgca cgtgcggtcg tccggggcag aggcgccgca cgcaccgacg acgggaaggc
     1261 tgtcccgcct gctggggccg ttccacgtca ccggtccgtt ctggtaccgg ctccacctgt
     1321 tcggaatgaa gatcctgccc tggtggatgg tcgggctcgt gatccacctc ttcaccgcgt
     1381 tcttcttttg cacgctgatc cgcatacgcc gggcgatcgc gagcaatctc gacgcggcga
     1441 tcggtcccgc cgactggtgg gagcgccagc gacgcatcca ccggacgatg ctcgccctcg
     1501 cctggtcgct cagcgagcgc tacgagtggc tcgccggcaa gacgcgcacc gacgccgagg
     1561 tggacaacct ggcggcgtgg cgcgccgtcg ccggaacgga ccgcggcctg atcgtcgtca
     1621 cggcccacgt cggcaactgg gagatcggct cggcgctgcc cgcgcgccgc gagcgccgtc
     1681 gcatccacct cgtgcgagag gaagagctcg atcctcgtgc gcagcgcttc atccggcagc
     1741 tgctgagccg ccagacggac gatcgataca cgacgcattt cgcgaccggc gatccgcggc
     1801 tcgggatcct gctccgcgaa gcgctcgagc ggggcgacgt cgtcgccctg caaggcgacc
     1861 gcccgcgggc cggcggcaag gtgctcgagg tgtcgttgtt cgggaggccg tttcgcgtcc
     1921 ccgtcggtcc gttcgcgctg gcgcgcgtcg ccgaggtgcc gctgttgccc gtgttcatgc
     1981 tgcgcgaggg gcgaacgaga tatcgcgtgc attttcgcca gccgattcgc gtcgcgcgca
     2041 gctccgaccg ggagcgggac catcgttcgg cggccgagcg gttcgccgcc gagatcgagt
     2101 gggcgatctc ggcagccccg catcagtggt tctgcttcaa cgagctttgg ccggggagtc
     2161 ctaccgggcg gcccgctcgg tctcgacgaa cgtgctgatc gagccggcga agcagcgctc
     2221 gccggcggat tcgacctccg cgtcgtagcg gtagagcggt ggcgcggccg cgctgcgccg
     2281 gacgtggacc cgcagcgtct gctcgacggc gaggaacggg ccgccgaact ccgcgcggcg
     2341 cacgcggacg agatagccga ggcgggcgcg acgggacggg ccctcgcgcc gcgcgcattc
     2401 gagcagcgcc gcggcctgcg ccgcgagctc gatcgccaga aacgacggtg cacgcccggc
     2461 cacggcaaac gggctcccct ccggaatccg cccggcgcac acgatgcgat ccgcgtcgag
     2521 ctcgagcacg cgatcgacga accgagcgac gccgtcgtgc ggcagcagcg ctgcgggatc
     2581 gggcgggagc ggcgtcacct ccgctccgtc cgtcgccggc gcagtcgacc ctcgctgacc
     2641 agccggccgg ctctttcgat gcggaatgcc gtggcttcgc cggcgagcgc gggctcgatg
     2701 cggacggtca gctcgtcgcc aggcaggatc ggccggacga accgcaacgc ctccgccccg
     2761 gcgatccatg cggcaccggg ctcggcgcgc tcgagcagat cggcgaccaa cgcgagctgt
     2821 ccgatcgcgg gcaggatcgg ccggtgcggg aagtgtccgc tgaagtaggg acagtccgcg
     2881 ggaacgcgga ccgagacaac ccgctccggt gcggtcgaac cgtcgtcgag cagctcgaac
     2941 gcggacacgc tcaccccacc cgatgaccgg cgccggctcg gactcgagcg cctcggagcc
     3001 gacgcgccgc cggccgcact ctaccagacg cgtctcctgc cgggcccggg cggcttgacc
     3061 ggcgcgaagc gattgtgctt cactcccggc cacacgggtc gtgacgggag gtttccatgg
     3121 cggatcaagt caagaagctg agctactgct atctgacgct gccgcaccgt gcgggccaag
     3181 gcgatcgcgt cctcggcgat ctcgagcgtg cgggtgtcaa tctgctggcg ttctccgcgt
     3241 ttccggacaa gggcgggaag gcgcagatcg atctggccgc cgacaacctc gccccgctgc
     3301 gcaagatcgc caagcagaac ggctggcgtc tgagcgcggc caagaaaggc ttcctcgtcc
     3361 aaggcgagga ccgggtcggt gcggtacggc ggcacatccg caagctcgcg caggagaaga
     3421 tcaacatcac ggccgccgac gcggtcgccg ccggcaaggg acgctacggg atgatcctgt
     3481 gggtcaagtc gaaggactac cgccgcgcag cgcaggtgct gcgggccaag tagccgacga
     3541 tggctcgcac gaaagccggc gactacagcc cgcaggttct gcggctgttc gaccggttcg
     3601 tccacggcga gatctcgcgt cgcgagtttc tcgatcgtgc cgcctcgctc gtcgtcgccg
     3661 gagtgagcgc ggcggcgttg ctcgacgctc tgaccccgga cttcgccgcc ggccagctga
     3721 tcgctgcgga cgactcgcgt ctcgcgacca gccgccccga gtacgactca ccgcgaggcg
     3781 gcggaaagat gcgcggctat ctcgcgcggg gggccgcggg acccgagcga ctacccgggg
     3841 tgctcgtgat ccacgagaac cgcgggctga atccgcacat cgaagacatc gcgcgccgtc
     3901 tcgccgtcga gggctatctc gccttcgcgc cggacgcgct tgcgccgctc gggggctacc
     3961 ccggcgacga ggacaaggcg cgcgagctgt tccgcgagct cgaccgcgac aagctcgtcg
     4021 aggacttcgt cgccgcgttc gagttcctcg acgctcaccc cggctgcacg ggcaaggtcg
     4081 gcgccgtcgg cttctgcttc ggtggcggca tggtcaaccg gctcgccgtg aggttgccga
     4141 agctcgacgc cggcgtcgcg ttctacggcg gtcaggtgcc ggccgatcag gcggcgaaga
     4201 tcgaggcccc gctgctgctg cactacgccg gaatcgacga tcggatcaat gccggctggc
     4261 cggcgtacaa agcggcgctc gagagcgccg gcgttcgctt cgaggcgttc gtttacgaag
     4321 gcgtgcacca cggattcaac aacgacacga cgccgcgctt cgacgaggaa gcggccgggc
     4381 tggcctggcg tcgaacgctc gagttcttcg acaagtacct gaggtgagcg ggtcatcgat
     4441 cgaccgacgg ccggcgggac gatctcgcga gcgaacgccg gacccacggc ctcaggtgat
     4501 cgaaatcgca cgttccgccg tcgagcaaac ggatcaggaa acggtgcgtc cggccggcat
     4561 cgacggcgag cttccagccg ttcagcctga agaacacgtc ggtcgcgaag aacgcgacgc
     4621 gcttgttccc gtccacgaac gggtggttca tcaggagcga ctcgaacagc gccgtgccca
     4681 tgtccgccag atcgcggtag tagccggtct gcggacggta gagcgcgctc tcgagcaagc
     4741 cggcgtcccg cacgccggac agaccgccga acctttcgac gaggcgctca tgcatggcga
     4801 gcccctcgtc gacggtcagg aacaggatcc gttcagcggg caagcaggcg gccgagcttc
     4861 tcgttttcga cgatcgagtc ctcgaggtgc cgctgcacga tcggtcgcac gcggcgccgc
     4921 tgcaggtatt cgcgaatcgc ctcggtgagg agtccggaga ggctctggtg cgactcctcg
     4981 gccagttcct tgaactcctc ccacaccgac gactcgactt tcgagctgac cttgatcgcg
     5041 gccatcgctt cctcctgacg cgtcaagata cgtcaagacg acacggactg ccacgagatc
     5101 gcgcagctga ccgtctatct cgaggaggtc gccgcggcgc ccaagctcga ccgtgatcgc
     5161 tctcctcgag gcgtcgacga catcgaccac gggtcccggt actgcgcggc ccggccatcg
     5221 ccgaccaggc ccaggacact cgtcatcggt ggcgcgaggt cggtcttcaa caccggtccc
     5281 cacaccacct gcacccgatg caatcggttc ttttcctcga gtggaagttg cataagatat
     5341 cacggttccg gacgaggccg agccatgcgc cgtgctcttc tctcctcgct gatcctgctg
     5401 tcgcccacgt gggcgttcgc gtcgaacacc gggccggtcg atctcgatgc cgcggcccgg
     5461 cggttcgcca cggcctcgtt ccccgagctg tacgcgctgt tgagcctgcc gaacgacgcg
     5521 cacttccccc gagacatcga gaagaacgtg gcattctgcg aggcggcgtt cgccaagcgc
     5581 ggattctcga cgcggcggct cacgacgccg ggcgcgccgc tcctgctcgc cgagcgcacc
     5641 ttcgatcgag gagagaagac cgtactgatc tacctccaga tcgacggcca accggtggac
     5701 ccggacgcgt gggatcaaga aagcccgtgg acgccggtgc tcaaggagcg cgacgcgagt
     5761 ggagcctggc ggatcattcc tgccgaccgg ctcgagggtg gcaccccgaa ccccgagtgg
     5821 cgcatcttcg cccgggccgc ctccgacgcg aagggtccgg tcgccgcatt cctggcctcg
     5881 ctcgacgccg cggcggcgct cgaggtcgcg cccgatttcg acatcaaggt gatcatggac
     5941 ttcgaggagg agctcggctc tcccaaccta ccgtccgcgg tcgaacgttt ccggagcgag
     6001 ctcgccgcgg accggctcgt catcttcgac ggaccgcgcc acatctcgaa ccggccgacg
     6061 ctgacctatg gcgcacgcgg catcgcggag atgacgctcg tcgtgtacgg cccgcgcacg
     6121 cccgtgcaca gcggcaatta cggcaactac acgccgaacc cggcgatgcg tctggcccgg
     6181 ctgctggctt cgatgaagga cgatcgcggc cgcgtcacca ttcccgggtt ctacgacggc
     6241 gtgcagctga cgcaggccga aaaggagacc ctacgccagg tgcccgacga cgagggcgag
     6301 atccgcaagt tcctcggcat cgccgaaccc gacgcgatcg gcgacaactt ccaggaatcg
     6361 ctgcagtacc cgacgctcaa catccgcggg ctcgatgcac tgcacgtcgg caacgaggca
     6421 cgcacgctga tccccgaccg ggcggtggcc gagatcgaca tccgcctggt gccgtcgagc
     6481 gacgccccgc ggctgatcgc gctcgtgacc gagcacgtcg cgaggcaagg gtacctcgtg
     6541 ctcgatcggg ccccggacga ggaggagcgg atgacccatg cgcggatcgc ctcgatccgc
     6601 agcagcgtct cctacggtcc gttccagacg ccgttcgagt caccgaccgg gaactggctg
     6661 cgccgcgcca tgacacgggc cttcggccac gagccgatcc agatccgcat gatgggcggt
     6721 tcgattccga tcgcgccatt cgtgaccacg ctcggcattc cggccgtggg tgtgccgacc
     6781 gtgaacccgg acaacaacca gcacgcggag aacgagaaca
//
LOCUS       DASPGN010000508         6029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_94363, whole genome shotgun sequence.
ACCESSION   DASPGN010000508 DASPGN010000000
VERSION     DASPGN010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94363"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="VD788_14715"
     CDS             <1..1183
                     /locus_tag="VD788_14715"
                     /inference="COORDINATES: protein motif:HMM:NF013636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HXV77563.1"
                     /translation="ATVEVDANPFPTHTDPDVGDFHRLLLPGDYTLGISAAGYAPAAI
                     PVQASTITAARYDVALVPLPTVLVATARRVIDGEAEPGEAVDLAVTLTNSGRAATGVS
                     ASLVPTGWYGDVTRAQATYLEIPTGGSGESESPHFGIAIDAATPAGHKAGYAVQWTAD
                     QGSGASGPIFVDVGAPACDSVAASDVPLPIDDQSTTSSTLDLPLDIELSRVEVSVDLT
                     HTYIGDLRLVLRSPTGTDIVLHDGSGGAATEISGTYGVDLTPVDSLELLVGEPALGAW
                     TLEVHDEAPTDTGTLNGWVLSPCGRPVEAHPPEMRLRELAVVGDTLQLDWWPYPGLDG
                     YRVYRSTTASDWDGFSDVTGADADPADTRFVDDASGVIHFYLVTGVGPRGEGPLGHFG
                     R"
     gene            complement(1196..2344)
                     /gene="rffA"
                     /locus_tag="VD788_14720"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(1196..2344)
                     /gene="rffA"
                     /locus_tag="VD788_14720"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF008687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HXV77564.1"
                     /translation="MKQPPGQIPFNRPLIVGRELDYIRQAVEAMHTSGDGPFTQRVAR
                     LLRDALGVERAMLTPSCTDALEMAGLLLDLGPGDEFVVPSFAFVSTVNAFVLRGARPV
                     FADIRPDTLNLDESQLEATITPLTRLVVPLHYAGVACEMDAITSIANAHGLSVVEDNA
                     HGLFGSYRGKPLGSFGGLAALSFHETKNFSCGEGGALLLNEPGFVERAEFIRDKGTDR
                     QRLFRGQVDKYTWVDVGSSFVLSDLLAAFLYGQLEQRERVLAERERIWRGYDGALRGA
                     IERHGVSLPVVPPHCRQGFHMYYLLLPGLAVRQRLVAHLARRGIASVFHYTPLHLSRM
                     GRRFGGRPGQCPVTERISETLLRLPFHLELTDDDQRRVVQSIFEFFGE"
     gene            2479..4377
                     /locus_tag="VD788_14725"
     CDS             2479..4377
                     /locus_tag="VD788_14725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV77565.1"
                     /translation="MLRCMGSVLVLLALVVVATQGSEEAGPSAEKQPSEAIEVRVVLI
                     EALVVDAEGNAVPDLTADEFQLAIGGVLTPIESLDVYCPAAAAGAAGRDGAAAGAPAA
                     SDRVPLASPPRIVFAVDYRHLEHHDRTKVLDRIGEMVGEGFETGEQEIMIAAIADRMR
                     VEQRFSRDLDEVLATLHRMDYDRTLFAVDSEASTGEEWFDQLSTLMDVLEVYDGPKAV
                     VLVSTVQSRDDVLEAWFDEVYERAAAARVTIYPAYARWMTFSDIRRGMYRAPGTAGGR
                     RILPALADGTGGSLPSTTDDLSLAYVRARRDLGCRYTVGFEVDSDVAGNSMELGLQVR
                     RPGVRVLCPASSRVRSDEERRQSRLRAAFADPQSLDSPLVRAQAIPLRPVDGKTWQTL
                     LAVQFPLPPRVGDNGFEVRASLAGSRAKTPPMIERHFPLSGAGEAGRPVTVLGDSPLE
                     SGSHMLTVAVSEPGSDDVVTTQTRVDVPAAPKDGVILQGPMLARVLDDNGEFLRRLEQ
                     AHAGTPPLEELLEDERLVELLLINEVRASDTLVAFWHACAAGKQSHSRPLAVERRIVT
                     AEGEPVRTLDRHTFRGAGDGRTRCDGTIDPIPAGTLAPGEYVIEVVVVDDDSGAELAR
                     ESAPVSLR"
     gene            complement(4408..4911)
                     /locus_tag="VD788_14730"
     CDS             complement(4408..4911)
                     /locus_tag="VD788_14730"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HXV77566.1"
                     /translation="MDETMAAWMQYAQPGEHHRFLRKLAGSWETRAKLWMQPGAPPVE
                     SSGSTVNELIMGGRFLRSDYSATFMDRPFQGMGLDGFDNQKRRYVGLWIDTMGTMMLT
                     FEGEADPDGATRTMFADFVDAVTGKPSKMKGVTTIVSDDEHRYESWVEGAEGETFKTM
                     EIVFTRR"
     gene            5184..5777
                     /locus_tag="VD788_14735"
     CDS             5184..5777
                     /locus_tag="VD788_14735"
                     /inference="COORDINATES: protein motif:HMM:NF014248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HXV77567.1"
                     /translation="MGKFGSLTIAALLVLTLVGCGKGVKQIPPDKGKKLVVPAWYLDP
                     PVDDDRLVGVASATSLDMQTAVDKAKQDGRTEIARQLELRVTGMSKRFIEETGLDDDA
                     ELLGMFTQVSRSVVSDALTGSRISKQELGRHGTGYRAYVMMEMPIGEANARFVERIRA
                     QERLYTRFRASEAFEELDRMVEEYEQWKNDQAAGLGR"
     gene            5774..>6029
                     /locus_tag="VD788_14740"
     CDS             5774..>6029
                     /locus_tag="VD788_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77568.1"
                     /translation="MSRPPRVVASIVAAMAALSPGTGVAESSELRAVEVAAEVYAAER
                     TPAEAKREALQRARDLAVARAAGIHVRAQQLRLVSEEPEAV"
BASE COUNT          935 a         2078 c         2081 g          935 t
ORIGIN      
        1 ggcgaccgtc gaggtcgatg ccaacccgtt cccgacccat accgaccccg acgtcggcga
       61 cttccatcgc ctgctactcc ccggcgatta cacgctcggg atctcggccg cggggtacgc
      121 gccggccgcg atcccggtcc aggccagcac gatcacggcg gcacgctacg atgtggccct
      181 cgtgccgctg ccgaccgttc tggtggcgac cgccaggcgg gtgatcgacg gcgaggcaga
      241 gccgggcgag gcggtcgatc tggccgtgac gctgaccaat tccggccgcg cggcgaccgg
      301 tgtctcggcc agcctggtgc cgacggggtg gtacggcgac gtcacccgcg cgcaggcgac
      361 ctacctcgag atcccgaccg gcggcagcgg cgagtccgag tccccccatt tcggcatcgc
      421 gatcgacgcg gcgacgccgg cgggccacaa ggccggctac gccgtccagt ggacggccga
      481 tcaggggtcg ggcgcgtccg gaccgatctt cgtcgacgtc ggcgcacccg cgtgcgacag
      541 tgtcgcggcg tccgacgtac cgctcccgat cgacgaccag tcgaccacga gcagcacgct
      601 cgaccttccg ctcgacatcg agctgtcacg ggtcgaggtg tcggtcgacc tgacgcatac
      661 ctatatcggc gacctgcggt tggtgctgcg ctcgccgacc gggaccgaca tcgtgctcca
      721 cgacggttcc gggggggcgg ccaccgagat ctcgggcacc tacggtgtcg atctgacgcc
      781 ggtcgactcg ctcgagctgc tcgtcggtga gccggcgctc ggcgcctgga cgctcgaggt
      841 ccacgacgag gctccgactg acacgggaac gctcaacggc tgggtcctgt cgccatgcgg
      901 gcgcccggtc gaggcgcacc cgcccgagat gcggctgcgc gagctcgccg tcgtcggcga
      961 caccctgcag ctcgactggt ggccctatcc cggactcgac ggctatcgcg tctaccgctc
     1021 gacgacggcg tccgattggg acgggttcag cgacgtcacc ggggccgatg ccgatccggc
     1081 cgacacccgc ttcgtcgacg acgcgtcggg cgtgatccac ttctacctgg tgaccggcgt
     1141 cggcccgcgc ggggaaggtc cactcggcca cttcgggcgt tgagccccgc cccgatcact
     1201 ccccgaagaa ctcgaagatc gactggacca cgcgacgctg atcgtcgtcg gtcagctcca
     1261 ggtggaacgg tagccgcagc agggtctcgc tgatgcgctc ggtcaccggg cactgccccg
     1321 gccgaccacc gaagcgacgc cccatcctcg acaggtgcag cggcgtgtag tggaagacgc
     1381 tcgcgattcc acgccgtgcc aggtgggcga ccagccgctg ccgcaccgca agcccgggaa
     1441 gcagcaggta gtacatgtgg aagccctgcc ggcagtgcgg cggaacgacc ggcaggctca
     1501 cgccgtgccg ctcgatcgca ccgcgcaggg caccgtcgta gcctcgccag atccgttccc
     1561 gctcggccag aacccgctcc cgttgctcga gctggccata cagaaaggcg gccagcaggt
     1621 cggacaggac gaagctcgac ccgacgtcga cccaggtgta cttgtcgacc tggccccgga
     1681 acagccgctg tcgatcggtc cccttgtcgc ggatgaactc ggcccgctcg acgaatcccg
     1741 gctcgttgag cagtaacgcc ccgccctcgc cgcagctgaa gttcttcgtc tcgtggaagc
     1801 tgagcgccgc caggccgccg aagctgccca gcggcttgcc gcgatagctg ccgaacagtc
     1861 cgtgggcgtt gtcctcgacg accgacagcc cgtgggcgtt ggcgatcgag gtgatcgcgt
     1921 ccatctcgca cgcgacaccg gcgtagtgca ggggaacgac gagtcgggtg agcggcgtga
     1981 tcgtcgcttc gagctggctt tcgtcgaggt tgagcgtgtc gggtcggatg tcggcgaaca
     2041 ccggacgggc gccgcgcagg acgaaggcat tgaccgtcga gacgaaggcg aacgacggga
     2101 cgacgaactc gtcccccggc ccgagatcga gcagcagtcc ggccatctcg agcgcgtcgg
     2161 tgcaggacgg cgtgagcatc gcgcgttcca ccccgagcgc gtcgcgaagc agtcgcgcga
     2221 cgcgttgcgt gaacggaccg tcgccgctgg tgtgcatcgc ctcgacggcc tgacggatgt
     2281 agtccaactc gcggccgacg atcagcggcc ggttgaacgg gatctggcct ggcggttgtt
     2341 tcatacggat tcgcggcgaa acgggccgat cagcttaggt gaccgcgcgc cggcctgtca
     2401 atcgacgttc cattcggacc cgcggtttga taccatttcg acaggtgtga cgcggaccgt
     2461 gtcgctgggg gtgaccgcat gctgcgatgc atgggttcgg tcctggtgct gttggccttg
     2521 gtcgtcgtcg cgacacaagg ctccgaagag gcgggcccgt ccgccgaaaa gcagccaagc
     2581 gaggcgatcg aagttcgggt ggtgctgatc gaggcgctcg tggtcgacgc cgaggggaac
     2641 gcggtgcccg acctgaccgc cgatgagttc cagctcgcca tcggtggcgt gctcacgccg
     2701 atcgagtccc tcgacgtcta ctgcccggcg gcagccgcgg gtgccgccgg tcgcgacggg
     2761 gcggccgccg gtgcgcccgc cgcgagcgat cgagtcccac tcgcctcgcc gccgcgcatc
     2821 gtcttcgcgg tcgactaccg gcatctcgag caccacgacc ggaccaaggt gctcgatcga
     2881 atcggtgaga tggtgggcga agggttcgag accggcgagc aggagatcat gatcgccgcg
     2941 atcgccgacc gcatgcgcgt cgaacagcgg ttcagccggg atctcgacga ggttctcgcc
     3001 acgctgcatc ggatggacta cgaccggacg ctgttcgccg tcgattccga ggcctcgacc
     3061 ggcgaggagt ggttcgacca gctgtccacc ttgatggacg tgctcgaggt gtacgacggg
     3121 cccaaggcag tcgtgctcgt ctccaccgtc caatcgcgcg acgacgtgct ggaggcatgg
     3181 ttcgacgagg tctacgagcg cgcggcggcg gcacgcgtca cgatctaccc ggcctatgcg
     3241 cgctggatga ccttctccga catccgccgc gggatgtatc gtgcaccggg aacggccggc
     3301 ggccggcgga tcctgccggc gctcgccgat ggcacgggcg gcagtctccc gtcgacgacc
     3361 gacgacctgt cgctggcgta cgtgcgcgcc cggcgcgatc tcggctgccg ctatacggtc
     3421 ggtttcgagg tcgactcgga cgtcgcgggc aactcgatgg agctcggcct gcaggtccga
     3481 cggccgggtg tccgcgttct gtgcccggcg agctcccgcg tccggtccga cgaggagcgg
     3541 cggcagtcgc ggctgcgcgc ggcgttcgcc gacccgcagt cgctcgacag cccgctggtt
     3601 cgcgcgcagg cgatcccgct gcgcccggtc gacggcaaga cgtggcagac cttgctcgcc
     3661 gtccagtttc ccctcccgcc tcgggtgggc gacaacggct tcgaggtgcg ggccagcctg
     3721 gccggctcgc gcgccaaaac gccgccgatg atcgagcgtc acttcccgct ttccggggcc
     3781 ggcgaggccg gacgaccggt cacggtgctc ggtgactctc cgctcgagtc cggctcgcac
     3841 atgctgaccg tcgccgtttc cgagccgggc agcgacgacg tggtcacgac tcagacccgg
     3901 gtcgacgtgc ccgctgcgcc gaaggacggg gtgatcctcc agggaccgat gctggcccgg
     3961 gtgctcgacg acaacggcga gttcctccgt cgactggagc aggcccacgc cggcacgccc
     4021 ccgctcgagg aactgctcga ggacgagcgc ctcgtcgagt tgctgctgat caacgaggtt
     4081 cgcgcgagcg ataccttggt cgcgttctgg cacgcgtgcg ccgccggcaa gcagtcgcac
     4141 tcccgaccgc tcgccgtcga acgccggatc gtcaccgccg agggcgagcc ggtccgaacg
     4201 ctcgaccgcc acacgttccg cggggctggc gacggacgaa cgcgctgcga cgggacgatc
     4261 gacccgatcc ccgccggtac gctcgccccc ggcgagtacg tcatcgaggt cgtcgtcgtc
     4321 gacgacgatt ccggggcgga gctggcccgc gaatcggctc cggtctcgct gcgctgaacg
     4381 acgtcggcgc cggggaccgc gttcgcgtca ccggcgtgtg aagacgatct ccatcgtctt
     4441 gaacgtctcg ccctccgcgc cctcgaccca cgattcgtac cggtgctcgt cgtcactgac
     4501 gatcgtcgtc acgcccttca tcttggacgg cttcccggtg acggcgtcga cgaagtcggc
     4561 gaacatcgtg cgtgtggcgc cgtcgggatc cgcctccccc tcgaaagtga gcatcatcgt
     4621 gcccatcgtg tcgatccaca gcccgacgta gcgtcgtttt tgattgtcga aaccgtcgag
     4681 gcccatcccc tgaaacggtc gatccatgaa ggtcgccgag tagtccgacc tgaggaagcg
     4741 cccgcccatg atcagctcgt tgacggtgct gccgctcgat tcgacgggcg gcgctccggg
     4801 ctgcatccag agcttcgccc gtgtctccca gctgccggcg agcttcctca ggaagcggtg
     4861 gtgctcaccg ggttgtgcgt attgcatcca ggccgccatc gtctcgtcca tctcgaacct
     4921 ccgtgctgag ctcgcccgct ccgggaaaac agacccccga cccgcccgga agtatggacc
     4981 gccggccaga cgaatacaag acgaaagtta cggcccacgc cttgcaaccc cgctcgttgg
     5041 tatcggggta aaccgcgatg atctcggcga gaacgggcgg attctccgga aacgcgcagg
     5101 tgagcgtgcc tcggggtgac atccgctcag gacattgcgg tccatgcgag gggacatgcc
     5161 tcgcacgcga ggagggcgaa gcgatgggga agttcggatc gttgacgatc gcggcgttgt
     5221 tggtcctgac gctggtcggc tgtggcaagg gggtgaagca gatcccgccc gacaagggca
     5281 agaagctggt cgtccccgcc tggtatctcg acccgccggt cgacgacgat cgtctggtcg
     5341 gcgtcgcgag cgcgacgtcg ctcgatatgc agaccgcggt cgacaaggcg aagcaggatg
     5401 gacgaaccga gatcgcacgg cagctcgagc tccgcgtcac cgggatgtcg aagcggttca
     5461 tcgaggagac cggtctcgac gacgatgcgg agctgctcgg catgttcacg caggtcagcc
     5521 gatcggtcgt ctccgacgcg ctgaccgggt cccggatctc caagcaggag ctcggccggc
     5581 acggaaccgg ttaccgggcc tacgtcatga tggaaatgcc gatcggcgag gccaacgcgc
     5641 gcttcgtcga gcggattcgc gcccaagaac ggctctacac ccggttccgg gcctccgagg
     5701 cgttcgagga gctcgaccgg atggtcgagg aatacgagca atggaagaac gaccaggccg
     5761 ccgggctggg acggtgagcc ggccgccccg cgtcgtagcg tcgatcgtcg cggcgatggc
     5821 cgccctttct ccaggaaccg gagtcgcgga gagctcggag ctccgggcgg tcgaggttgc
     5881 cgccgaagtc tacgcggccg agcgcacgcc cgccgaggcg aaacgggagg cgctgcaacg
     5941 cgcacgagac ctggccgtcg cccgcgccgc cgggatccac gtgagggcac agcagcttcg
     6001 cctggtctcg gaggagcccg aggccgtgc
//
LOCUS       DASPGN010000509         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1617146, whole genome shotgun sequence.
ACCESSION   DASPGN010000509 DASPGN010000000
VERSION     DASPGN010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1617146"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VD788_14745"
     CDS             <1..441
                     /locus_tag="VD788_14745"
                     /inference="COORDINATES: protein motif:HMM:NF020672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HXV77569.1"
                     /translation="EGELRLEADRVARAGHRVVLEGADQQVAERVERAFAAGGLEPPD
                     PDRLAPIARGSDLQKILALLIARGDLVRIHDGKLFHAAALRELIRRLAEYGRQSPTID
                     VAAFKTLAGVTRKHAIPLLEHLDATRVTRRVGDRREILVREGSG"
     gene            497..706
                     /locus_tag="VD788_14750"
     CDS             497..706
                     /locus_tag="VD788_14750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXV77570.1"
                     /translation="MAPLALFGTFGFQELIAILAIVVFLFGARKIPEIARGLGEGIRG
                     FRSSLKGEDEQGDGRDGKVQKHSEP"
     gene            complement(720..>2853)
                     /locus_tag="VD788_14755"
     CDS             complement(720..>2853)
                     /locus_tag="VD788_14755"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXV77571.1"
                     /translation="VLHGPGAAIAARAASWLVARGSRADHARRFFHLAAGCAGTLLAS
                     LGYLAVGGTVPFAGGTGGWVGVCAFAALWTAADRVSRALEAELPVSAPVVDDWPGWRR
                     GALTHGAGALLGGALASTHALLGGKAWWALPTVLLAFWLLPRLAPQRRSATRRTGPVD
                     RAIARAIAISLADEKPPPVRARRLCRVALDLGRSVQMTGTELDALELAAWLTDLERLE
                     PPPPPRHWPEARRFAAPMPARFDPRSVMDAIGLPASVQEMVVHVGERWDGTGGPDGLC
                     GARIPLGARVLAVARDFVSMTTPTHDRHALTRQRTLAELRGEAGRAFDPRIVDALAND
                     IGGRRVRAHDETSDRREHDDTPPLRGKPDGERLSEVRYELDALYGIRGCGKLPVDLDE
                     TLVLILEKLKGLVPHVTASVWLFDPEHDELHPRFVRGEGEATLRATTLPAERSFSGRV
                     AQRRRTATFHSGGPASDLAFDLDGLADDPALAPLRTALAAPLVTAGGCRGALTLYGRA
                     GHRFDTAERRALVAVAAHVAEAVETAKTRSRPALDSLTDPLTGLPNGRYLEIFAADLA
                     PETSQAGFGLLGFRIHDLDRVYELCGVAAGERLLGVLARRLAAHCVPSEVPVRYGHDL
                     FLVLSPIGAQHELVRRWDSLLRATEGSAIELRAGQFHRPRLASAHASCPDDGAGLDAL
                     LDVLGTRLGLAFDRGHKIVPFRAMRSAG"
BASE COUNT          430 a         1024 c         1050 g          349 t
ORIGIN      
        1 gaaggagagc tacggctcga ggcggaccgg gtcgctcgcg cggggcatcg cgtcgtactc
       61 gagggggcgg accaacaggt cgccgagcgc gtcgagcgcg cgttcgcggc gggcggtctc
      121 gagccacccg atcccgaccg actcgcgccg atcgcccgag gctccgacct gcagaagatc
      181 ctcgccctgt tgatcgcccg cggcgacctg gtccggatcc acgacggcaa gctgttccac
      241 gccgccgccc tgcgcgagct gatccgccgg ctggccgagt acggtcgcca atcgccgacg
      301 atcgacgtcg ccgcgttcaa gacgctcgcg ggcgtgacgc ggaaacacgc gatcccgctc
      361 ctcgagcacc tggacgcgac gcgggtgacc cgtcgcgtgg gcgaccggcg cgagatcctg
      421 gttcgcgagg ggtccggcta gtgccaaccc cggatttgct atatgttgaa cggtgtaagg
      481 gtcttaggag accgccatgg cacccctggc cttgttcggg acgttcggct tccaagagct
      541 gatcgcaatc ctggcgatcg tcgtcttcct gttcggagcg cgcaagatcc cggagatcgc
      601 ccgcgggctc ggggagggta tccgcggctt ccgttcgtcg ctcaagggcg aggacgaaca
      661 aggagacggg cgcgacggca aggtgcaaaa gcactccgag ccgtaacctc gatcgccggc
      721 taacccgcac tccgcatcgc gcggaacggc acgatcttgt gcccgcggtc gaacgccaag
      781 ccgagccgcg ttccgagcac gtcgagcagc gcgtcgagac cggcgccgtc gtcgggacag
      841 ctcgcgtggg ccgaagcgag gcgcggccga tggaactggc cggcccgtag ctcgatcgca
      901 cttccctcgg tggcccgcag caagctgtcc caccggcgga cgagctcgtg ctgcgcaccg
      961 atcgggctga gcacgaggaa caggtcgtgc ccgtagcgca cgggaacctc ggacgggacg
     1021 caatgggcgg cgagccggcg ggcgagaacg ccgagcaggc gttccccggc cgccacgccg
     1081 cacagctcgt agacccggtc gagatcgtgg atgcggaagc ccagcagtcc gaatcccgcc
     1141 tgcgacgtct cgggagcgag gtcggcggcg aatatctcga ggtagcgccc gttggggagc
     1201 ccggtgagcg ggtcggtcag gctgtcgagc gccggccgcg acctcgtctt ggccgtctcc
     1261 accgcctccg ccacgtgggc cgcgaccgcg accagcgcgc ggcgttcggc cgtgtcgaac
     1321 cgatggccgg cgcggccgta cagggtcagc gcgccacggc agccgcccgc cgtgacgagc
     1381 ggcgcggcga gcgcggtgcg cagcggggcg agcgcggggt cgtcggcgag cccgtcgagg
     1441 tcgaacgcga gatcggacgc ggggccgccc gagtggaagg tcgccgtccg cctgcgttgc
     1501 gccacccgac cggagaagct gcgctcggcc ggcagcgtgg tcgcgcgcaa cgtcgcttcc
     1561 ccttccccgc gtacgaagcg cggatgcagc tcgtcgtgct cgggatcgaa cagccaaacg
     1621 gaggcggtca catggggcac gagccccttc agcttctcga ggatcaacac cagcgtctcg
     1681 tcgaggtcga ccggcagctt gccgcacccg cggatgccgt agagggcgtc tagctcgtac
     1741 cgtacctccg acagccgctc gccatcgggc ttgccgcgga ggggcggggt gtcgtcgtgt
     1801 tcgcgccggt cgctcgtctc gtcgtgggcg cggacccgcc tcccgccgat gtcgttggcc
     1861 agcgcatcga cgatccgcgg atcgaacgcg cgcccggcct cgccgcgcag ctcggccaac
     1921 gtccgctgcc gcgtcagcgc gtgccgatcg tgagtcgggg tcgtcatcga gacgaagtcg
     1981 cgcgccacgg cgagcacacg ggcaccgagc ggaatacgcg cgccgcacag accgtccgga
     2041 ccgcccgtgc cgtcccagcg ctcgccgacg tgcaccacca tctcctgcac cgacgcgggc
     2101 aacccgatcg cgtccatcac ggaccgtggg tcgaaccggg ccggcatcgg cgcggcgaag
     2161 cggcgggcct ccggccagtg gcgcggcggc ggaggcggtt cgagacgctc gagatcggtg
     2221 agccaggccg ccagctcgag ggcgtcgagc tcggtgccgg tcatctgtac gctccggccg
     2281 agatcgagcg cgacccgaca gagtcggcgc gcacgcacgg gcggcggctt ctcgtcggcc
     2341 agcgagatcg cgatggcgcg cgcgatcgcc cgatcgaccg gccccgtccg ccgggtggcc
     2401 gagcgtcgtt gcggggccag gcgcggaagg agccagaacg cgaggaggac ggtggggagg
     2461 gcccaccagg ccttcccccc cagcagggcg tgggtcgacg cgagcgcgcc accgagtagc
     2521 gctccggcgc cgtgggtcaa cgcgccgcga cgccatcccg gccaatcgtc cacgacgggt
     2581 gcggagacgg gcagctcggc ctcgagagcg cgggagacgc gatccgcggc ggtccacagc
     2641 gccgcgaagg cgcacacccc gacccaaccg cccgtaccgc cggcgaacgg caccgtcccg
     2701 ccgacggcca ggtagccgag cgaggcgagc aacgtcccgg cgcagccggc ggccaagtgg
     2761 aagaaccgcc gcgcgtgatc cgccctcgat ccgcgtgcga cgagccacga cgcggcgcgc
     2821 gcggcgatcg ccgcgccggg gccgtggaga acg
//
LOCUS       DASPGN010000510         5027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_94750, whole genome shotgun sequence.
ACCESSION   DASPGN010000510 DASPGN010000000
VERSION     DASPGN010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94750"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3435
                     /locus_tag="VD788_14760"
     CDS             <1..3435
                     /locus_tag="VD788_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77572.1"
                     /translation="RGTLALGIHDTLTLELRFQAAAPGQFLTVAPTTARLDLDLDVED
                     GGTPEAWTESFEDGTFGRFQPDNLDEGLDSLVLADGYRCAESDPDNPASLVYGNQTSL
                     TCYPGPDGLGPDPLHWKIDGPTDPDSAALDGGRGFDGQHALYWGVPLLPPPYASETHS
                     MPTAALEAAGLTDPVHLRAGKVCSVTRTTPCTDAVDCPAGEACERLRPALTFRHQVGF
                     HEFRFDKFLGRAVVQVQPADASGDPAGPWETLDPDYNGYDQDSFTLVSSCHFDPIDDG
                     STEDDLFEPGGPYGASSTCQGTRSWGQAGVAYSTPFDPTSTGNLPGPALEGASGAGTW
                     VETRVDLSRYAGRSVRVRFLATTIKFGSVETTYDYYVSPLWDDGWWIDAVEIADARTA
                     PGAISIDAAPNLGLPGLSDSDLDGAPQACDVCPAAADPGQEDFDLDGRGDACDNCRVA
                     YNPDQLDADLDGAGDACDRCPAGDADDGDADGLACAADNCPADANAGQADADGDGAGD
                     ACDACPADARDDADGDGVCGDLDPCTGGLEDRGTLQIFAPSAPAPSRAFDGTFSPSGT
                     FVVAVVRDSLGTQDRLRVFRLPLDGVPLEPTPDGFDAWALSPVEDLVVGLRGGLLSAA
                     PLDGGPTWQVANLGLSLPGEVPTSLAFSRDGQFVRVRTELDAVYAIPLHDGAPARLDP
                     ALGVPLLAVHEAQDNRLVLEYVNGELYAVDPGDAVPVPLQGPAELRWIDAPSQTDAAL
                     VFDETTPSLVRVPLDGSAPSVLSGGEVVACVTNGCATTVRTLGDHVYFEAAGPSSFNA
                     VWAAPLSGGSLQRFDIEGEAVLADASLGVVGVDTATVLFRAAAGLFVGPATGGPSQQV
                     VIEPSQIVQFAVDDAAQNVVYVVDPLDQDDALYRTGLDGGDPELLFEEEWIRFAEADG
                     RLPAGDLVIFHGGDALWEVPAAGGTPTERGAPFQPWNVGLEPQLALSPARDRVLVTDY
                     AVEDSGGYPRLGLLDVVADADADGLLDLCEGCPDSFDAVDADHDGAGASCDCDDANGF
                     VFPGATEVHDGADNDCPGVPGHGLVDEVSDLVATGPDTFAWFAQPGALLYEIARAETP
                     TFEPGCARFESMAPARTDADVPAAGSAYYYLVRASLPGTGSWGAGPSGAERTGVCD"
     gene            complement(3483..4070)
                     /locus_tag="VD788_14765"
     CDS             complement(3483..4070)
                     /locus_tag="VD788_14765"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HXV77573.1"
                     /translation="MWKRLLAGVALGALVVALPATAKDKEVKDYSKTIDDFKKIPDVA
                     KFFESAHGYAVFPSIGKGGLGIGGSHGKGQVYRAGQVIGFTSTSDVSIGLQAGGQAYS
                     QIIFFQNEEALSQFTGGNFEFGASAEAIAVQAKAEAGASTEGSSASASGSKTADQATA
                     GAEYQNGMAIFTMAKGGLMYAATIAGQKYTYKPKE"
     gene            4288..>5027
                     /locus_tag="VD788_14770"
     CDS             4288..>5027
                     /locus_tag="VD788_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77574.1"
                     /translation="MLRTRRAYAGVVFAVGLLGVPSSPAEVTVRVDAPRCAPDGGSGT
                     ESDPYCRIQDAICAIKDTGGGTVLVHPGIYRESLRMFPRVSVVSTDGPAVTTIDASGI
                     PCTTRFCLPSQVNAVCSTVVFGEGATPADRLDGFRITGGSGLYREFPASSPPDAVAGG
                     GIFVFQGSPTISRNEIVDNVLAHGSTDEYWGAGIYVFGSVPAAPIRPTITDNLIAGNV
                     ASSPEGRPNSPTHSFGGGIYTSWYAAAR"
BASE COUNT          677 a         1795 c         1758 g          797 t
ORIGIN      
        1 cgcggtacgc tcgcgctcgg cattcacgac acgctgacgc tcgagttgcg cttccaggcc
       61 gcggcccccg gtcagttcct gacggtcgcc cccacgacgg cgcggctcga cctcgatctg
      121 gacgtcgagg acggtgggac ccccgaagcc tggaccgaga gcttcgagga cggcacgttc
      181 ggccgcttcc agccggacaa cctcgacgaa ggcctcgatt cgctcgtcct ggccgacggc
      241 tatcgctgcg cggagagcga tccggacaat cccgcctcgt tggtctacgg aaaccagacg
      301 tcgttgacct gctacccggg tccggatgga ctcggccccg acccgctcca ctggaaaatc
      361 gacggcccga cggatcccga ctccgcggcg ctcgacggcg ggcgcggctt cgacggccag
      421 cacgcgctgt actggggcgt cccgctgttg ccgcccccgt acgcctcgga gacgcactcg
      481 atgccgacgg ccgcgctcga ggctgccggg ctgaccgacc ccgttcacct gcgcgccggg
      541 aaggtctgct ccgtgacgcg cacgacgccg tgcaccgacg cggtcgactg cccggccggt
      601 gaggcgtgcg agcgcctgcg gccggcgttg acgttccgcc accaggtcgg gttccacgag
      661 ttccggttcg acaagttcct cggacgtgcc gtcgtacagg tccagccggc cgacgcgagc
      721 ggcgatccgg ccgggccgtg ggagacgctc gacccggact acaacggcta cgaccaggac
      781 agcttcaccc tggtctcgag ctgccacttc gacccgatcg acgacgggtc gaccgaggac
      841 gacctgttcg aacccggcgg gccgtacggg gcgtcgtcga cgtgccaggg cacgcgctcg
      901 tggggccagg cgggcgtcgc gtactcgacg ccgttcgacc cgacctcgac cggaaacctc
      961 ccgggcccgg cgctcgaagg cgcgtccggc gcggggacct gggtggagac tcgcgtcgac
     1021 ctgagccgct atgccggccg cagcgtgcgg gtccggttcc tcgcgaccac gatcaagttc
     1081 gggtcggtcg agaccacgta cgactactac gtttccccac tgtgggacga cggctggtgg
     1141 atcgacgcgg tcgagatcgc cgatgcgcgg acagcacccg gcgcgatctc gatcgacgcc
     1201 gcgccgaacc tcggcctgcc cggcctgagc gattccgatc tcgacggtgc gccgcaggcc
     1261 tgcgacgtct gtcccgcggc cgccgacccg gggcaggaag atttcgatct cgacgggcgg
     1321 ggcgacgcgt gcgacaactg ccgcgtcgct tacaacccgg accagctcga cgcggacctc
     1381 gacggcgcgg gcgacgcctg cgaccgttgt cccgcgggag acgcggacga cggggacgcg
     1441 gacggcctgg cctgcgcggc ggacaactgt cccgccgacg ccaacgccgg acaggcggat
     1501 gccgacgggg acggcgcggg cgacgcctgc gacgcctgtc ccgcagacgc gcgcgacgac
     1561 gcggacgggg acggcgtctg cggcgatctc gacccctgta ccggcggact ggaggaccgc
     1621 gggacgttgc agatcttcgc gccgtctgcc cccgcgccct cgcgcgcgtt cgacggcacg
     1681 ttctcgcctt cggggacgtt cgtcgtcgcg gtcgtgcggg attcgctcgg cacgcaggat
     1741 cgcctgcgcg tattccggct cccgctcgac ggggtgcccc tcgagccgac gccggacgga
     1801 ttcgacgcct gggcgctctc gccggtcgag gacctcgtcg tcgggctgcg cggtgggctg
     1861 ctctcggccg cgccgctcga cggcggtccg acgtggcagg tcgcaaacct cggtctgtcc
     1921 cttcccggag aggtgccgac gtcgctcgcg ttctcccgcg acgggcaatt cgtccgggtc
     1981 cggacggaac tggacgccgt ctacgcgatc ccgcttcacg acggcgcacc cgcgcggctc
     2041 gaccccgcgc tcggcgtgcc gctgctcgcg gtgcacgaag cacaggacaa ccgcctggtg
     2101 ctcgagtacg tgaacggcga gttgtacgcc gtcgatcccg gcgacgcggt ccccgttccg
     2161 ttgcaggggc cggcggagct ccgctggatc gacgcaccct cccaaaccga tgccgccctg
     2221 gtgttcgacg agaccacgcc atctctcgtc cgcgtcccgc tcgacggctc cgcgccgtcg
     2281 gtgctgtcgg gcggcgaggt cgtggcctgc gtcacgaacg gctgcgcgac gacggtgcgg
     2341 acgctcggcg accacgtcta cttcgaggcc gccggtccat cctcgttcaa cgcagtttgg
     2401 gcggcgcccc tgtcgggcgg cagcctgcag cgattcgaca tcgaaggcga ggcggttctc
     2461 gccgacgctt cgttgggcgt cgtcggggtg gacaccgcga cggtcctgtt ccgggccgcg
     2521 gccggcctgt tcgtcggccc ggccaccggc ggaccgtcgc agcaggtcgt catcgagccg
     2581 tcgcagatcg tgcagttcgc cgtcgacgac gccgcgcaga acgtcgtcta cgtcgtcgat
     2641 ccgctggacc aggacgatgc gctctaccgg accgggctcg acggcggcga tccggagttg
     2701 ctcttcgagg aagagtggat ccgctttgcc gaggccgacg ggcggctacc cgctggcgac
     2761 ctcgtgatct tccacggagg cgacgcgttg tgggaggttc cggcggccgg cgggacgccg
     2821 acggagcgcg gggccccgtt tcagccgtgg aacgtcggcc tcgagcccca gctcgcgctc
     2881 tccccggcgc gcgatcgggt gctggtgacc gactacgcgg tcgaggattc cggcgggtat
     2941 ccccgcctcg gtctgctcga cgtcgtcgcc gacgcggacg cggacggcct gctcgacctc
     3001 tgcgaaggtt gtccggacag cttcgacgcg gtcgacgccg accacgatgg tgccggcgcg
     3061 agctgcgact gcgacgacgc gaacggcttc gtcttcccgg gcgcgaccga ggtccacgac
     3121 ggcgcggaca acgactgccc cggcgtaccg ggccacggcc tcgtcgacga ggtgtcggac
     3181 ctcgtcgcga ccggtcccga cacgttcgca tggttcgcgc agccgggcgc actgctctac
     3241 gagatcgcgc gcgcggagac gcccaccttc gaaccgggtt gcgcgcggtt cgagtcgatg
     3301 gcgcccgcgc ggaccgacgc cgacgttccc gcggcgggtt ccgcgtacta ctacctggtg
     3361 cgcgcgtcgc tgccggggac ggggtcctgg ggcgccggtc cgtcgggcgc ggagcgcacg
     3421 ggcgtctgcg actgaagccg cgcgccgctc aggcgccaga cgttcggctg aagccccctg
     3481 gtctactcct tgggcttgta ggtgtatttt tgtcctgcaa tcgtcgcggc gtacatcaga
     3541 ccgcccttgg ccatggtgaa gatcgccatc ccgttctggt actctgcgcc ggccgtggcc
     3601 tggtcggcgg tcttggaacc gctggcgctc gcgctgcttc cctccgtgct cgcgccggcc
     3661 tcggccttgg cctggaccgc aatcgcttcc gcggaagctc cgaactcgaa gttcccgccc
     3721 gtgaattggc tgagggcctc ctcgttctgg aagaagatga tctgggagta ggcctgtccg
     3781 ccggcctgga gaccgatcga gacgtcgctc gtcgaggtga agccgatgac ctgaccggcg
     3841 cggtacacct gtcccttccc gtgcgagccg ccgatcccga ggccaccctt gccgatcgac
     3901 ggaaacaccg cgtagccgtg tgcgctctcg aagaatttgg cgacgtccgg gatcttcttg
     3961 aagtcgtcga tggtcttgga gtagtccttg acctccttgt ctttggccgt cgccggcagg
     4021 gcgaccacca gggcgccgag agcgactccg gcgagcagac gcttccacat gctcgttctc
     4081 ctttggtttg ccgagtggcg actccttcct tatagaccat ctcgatgcac tctcacaact
     4141 cgatcgtccg cggaccgctc ccgattctga cgacgacctt caccggcggc ggcccgacgt
     4201 cgtatgaaag gcgtataaag gacagggacc gtgcccggcc gggcgcggtg gtaggggtcc
     4261 ggagagtcga cctgggggag tgagagggtg cttcgcactc ggcgggcgta cgcgggtgtc
     4321 gtcttcgcgg tgggtctgct cggggtcccg tcgtctcccg ccgaggtcac ggtccgggtc
     4381 gacgccccgc gctgcgcgcc cgacggcggc agcggcacgg aatccgatcc ctactgccgg
     4441 atccaggacg cgatctgcgc catcaaggac acgggcggcg ggacggtcct ggtgcacccg
     4501 ggcatctacc gcgagtcgct ccgcatgttc ccgcgggtga gcgtcgtttc gaccgacggg
     4561 ccggccgtga ccacgatcga cgcctcgggg atcccgtgca cgacccgctt ctgtctgccg
     4621 agccaggtga acgccgtctg ctcgaccgtg gtcttcgggg aaggagcgac gccggcggac
     4681 cgtctcgacg gcttccgcat caccggcgga tcgggtctct accgggagtt ccccgcatcg
     4741 agcccaccgg acgccgtcgc gggcggcggg atcttcgtgt tccagggctc gcccacgatc
     4801 agccgcaacg agatcgtgga caacgtgctg gcgcacgggt cgaccgacga gtactggggc
     4861 gccggaatct acgtcttcgg atcggtcccg gcggcgccga tccgcccgac gatcaccgac
     4921 aacctgatcg cgggtaacgt cgcgagctcg ccggagggcc gacccaactc gccgacgcac
     4981 tcgttcgggg gcggaatcta cacgtcgtgg tacgcggccg cccggat
//
LOCUS       DASPGN010000511        17530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_914676, whole genome shotgun sequence.
ACCESSION   DASPGN010000511 DASPGN010000000
VERSION     DASPGN010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 17530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17530
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914676"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..2695)
                     /locus_tag="VD788_14775"
     CDS             complement(137..2695)
                     /locus_tag="VD788_14775"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXV77575.1"
                     /translation="MGPNDSVTGSVSQAEVREALDELPRYFSDELAPLMVSESVETLM
                     RCPPDFAVTAIRGWVATQGSRPGSDATVGDYLFHALKKLHQMIEYELIDDKKMTAFLR
                     LVSQQVLPLCPEHERATLEQNLGRLGDVTRAGPVASVGILHRQPGSRGGPSPGAGGAP
                     VGASGPSASVMPAAPPITMPSIPSLPLPEGGESVELARGLRRLTVMLRHLGRAVGGAE
                     RAEATTAGEPRMSSYALATAAAHSRDTREFARYLEQLEEIGVEGGMPEVFRTLGQAVP
                     GWTVGDDAPAPASSSERAMQHIMSMAEQSGEGLQRTTDIVQAAVTHFNDGALVQAAKM
                     LALARQSLQRMKLDPEVVGGILRRSQDTLSADRLRQFAERPEKHGLLSQVMSLFPAYT
                     VDGLLDAVAVEERRDQRRMMLALLEAHGQATRRAALDRLQACADGRLEDAFGYFQRNL
                     VFLLRRIPPADDDTREIEIGYYGELSSLHYPMLLVREAIGALSQLKSPAVERVLITRL
                     EQVEQALGEKTEIDTANELRALLDRIAAGLARQQSTSANRAVVKHAFQRRPVLGNALA
                     RVAELSGNDLSDDSEIVDLLLRALKREVPMKVLGFVMPKNQSSIVQLSRALAGTPTAD
                     VKAMLQRIASGFPEQEFGREAAAILSGFDASGLPEKKSRRETHRGLAGDVELFGLPNL
                     LQSLADSSVTGRLTLRNEHDEQFATVEIRDGRVQGCANGRLQGEDAFYQLFETPVPGT
                     FEFQPSDPPPQATDQPPMLDVQSGLLEALRRYDEFQMARAVAPDDVELRPTGQKPTSM
                     EGESDVEFLRGIWAQAAKGISPAACEQSVPVDTYRVRRLYAHWLESGALQPVNG"
     gene            2902..4221
                     /locus_tag="VD788_14780"
     CDS             2902..4221
                     /locus_tag="VD788_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77576.1"
                     /translation="MRREPRSGQRGSAMIMAVFVLALLASVGAALLFITDNELKMSQA
                     DVASKQVFFLSEGGLEAGRHEIFLMNKNSANVRDLSEELQTVSSDGTIDFDPTSLAPT
                     YDSSGQVTGFTGFNDDVPLKSMTALADGWYVTFMQNDPAEGVSTVVDDNDRVLLTAMA
                     VRNDRRVEMTRALVERLDTFAIPPSTITILGPDAEFNGGSSSAKFYVGDDHGPHCPPG
                     TSGNVPTVGVIGGGSLDTADDGMIKPDTYESGGVTGLPTMSDLTTDPDLPELWTNCEL
                     LVELAQIIHASADLIGDTNTPRDSLGVPGAEKVVYINGDYEINGNWDGGGLLLVTGDL
                     EFSGQSGWQGPIFVIGTGDFLRSGAGNGEISGGVVVANVAGPDRTLFTADDCAGQDGV
                     SGTSDDGVAGSRYVNDGGGTGITGYCSAYFTDWQSLRPFKLLSFLQE"
     gene            4251..4910
                     /locus_tag="VD788_14785"
     CDS             4251..4910
                     /locus_tag="VD788_14785"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HXV77577.1"
                     /translation="MQRRPTVRPATVAAGGARRDLDRGDRELLRVIDAEFDRAARAAA
                     HWLACAPGCDACCHGPFPITPLDVRRLVAGLGRLERRDPRRAGAIRARAQAAVGRMAA
                     GYPGDPDSGALDDDSAALDRFFDLHAALACPALDPRSGRCELYAWRPVSCRSYGPPAR
                     YAGDDLPPCDLCFRGAPVEVVERCRVEPDPDGIEQALIASLGVAPDDEWETLIAFALV
                     R"
     gene            4954..6078
                     /locus_tag="VD788_14790"
     CDS             4954..6078
                     /locus_tag="VD788_14790"
                     /inference="COORDINATES: protein motif:HMM:NF044723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HXV77578.1"
                     /translation="MVTPKSEKARKDRTTVKAIDGVAGEIAARISKLSKPEVSFPVRS
                     LGNVRYDPTRGYFEIGREKSTRTLTVNTAKTFAQTLKMMALSKELVRTDDFATKRDAY
                     YQSKNWGEAKFDEQTESDTVMDDIEAMFSVHGVSREQLRFIPDEHGGAVAGELIVFDQ
                     DLETGKTERIDCTRFGSGAYSIPSLTEHLTFETGAKFVLCVETGGMFQRLQSHKYWKK
                     AHCILVSMAGVPTRATRRFVRRLSEQCKLPVYAFVDCDPYGISNIYRTLKVGSGNAAH
                     INRFFCVPDARFLGVTPDDIVRYELPTHPLKEVDVKRAKDALKNDPFFKRHKPWQQAL
                     DKLLSMGVRAEQQSLARWGLNYVIDEYLPAKLAAPEKFLP"
     gene            complement(6080..6631)
                     /locus_tag="VD788_14795"
     CDS             complement(6080..6631)
                     /locus_tag="VD788_14795"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HXV77579.1"
                     /translation="MSDRLAPGFLVAAPQLLDPNFRRSVVLLLQQGRDGAIGVVINHE
                     SPLLLKDLCDDHAIDYSGDPDKRVRKGGPVQPEQGLVLYGAEHRDPEGRPVIDGLHVS
                     ASRATLERLCRLARGRFHCYSGYAGWGPGQLEREIGEGSWIVAPVDTAAILDGSPEEA
                     WVGTLRGIGIDPAALVPGGDVEA"
     gene            6722..6796
                     /locus_tag="VD788_14800"
     tRNA            6722..6796
                     /locus_tag="VD788_14800"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6754..6756,aa:Cys,seq:gca)
     gene            7113..9077
                     /locus_tag="VD788_14805"
     CDS             7113..9077
                     /locus_tag="VD788_14805"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXV77580.1"
                     /translation="MNVMSNGSLCARSALAVLVVAVLSASPALAGTISLAWDPVTHPD
                     LAGYRVFYGVNASNLDQSVDVGTNAQATLSNLADCTNYFVAVKARGTDGSLSPQFSNQ
                     ISGWARPVVASVSPPSIQRTTQMALTVSGVNFMPGASVSFSDPAIVVNGVTVNGCSQL
                     VVDVTVPDTAAVGTTDLTVVNPDQVFGQGSGVLTVTNALPNGQINQPASDVTIDEGDT
                     VTFAATGTDPDSDLPLGYAWDFGDPAIANSNQEDPGAVRFDNPGSHQVTLTVTDALGG
                     VDPTPAMVTVNVTPAQAPSISNVAVPTVGSTTATVTWTTNEVADSRVLYRRPGDTVFQ
                     QTPIDTTQVTSHSVQLEGLMPNTEYEFSVRSTDADGLTTTQPAATTFTTQPNSFTYLR
                     IEAESGPIAAPAQTQNGVDAFAGVYVRLAPGTASGTAGSPAGTWDYGFNLTSPATYYF
                     WYRLYAPSSGSSRWFESVDGGSFAAIAPPTTASWEWVEGRSFGLAAGQHRVTLGGGDA
                     QARVDRILITNDAGFRPTEGPGGDVSPPAPVNALNAAPDDTTVALSWTNPTDSGPLRV
                     VVRFTTGPSHPANPQDGQPLIDRAAPAGASDGVLHTGLANGTTYRYSVFVVDQWGNVS
                     SRATTSGTPVAAVIPLDPVTNLRRTDTLSP"
     gene            complement(9131..11275)
                     /gene="glgP"
                     /locus_tag="VD788_14810"
     CDS             complement(9131..11275)
                     /gene="glgP"
                     /locus_tag="VD788_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HXV77581.1"
                     /translation="MQPREIKTKPTASIEIPVQFSRLMDIAYNLFWAWSQPTRALFDM
                     IDNARWRHYRNPIELLIDLEPQRWHELQRDRDFIRTYRAIVEEFDLYMAPDEPTWFDS
                     RFPGYDGGPVAYFSTEYGWHECLQMYSGGLGVLSGDHSKAASDLDLPFIGIGLMYQHG
                     YFRQTIDADGQQQHFYPDYDFHRVPLLPVVDEQNGELRVTIELAGRDVQLRAWKASVG
                     RVQVLLLDTDLAANHPADRPITSVLYVQGREMRLCQEWVLGVGGVEFVRALGIRPAVW
                     HMNEGHSALLSFQRLRRLTEVDGLSWDEARLRVNENAVFTTHTPVPAGNETFDRELVR
                     QYVESWAAPGRVPVDSLLALGRARDDGGDESFNMTALAIRTSRRANGVSELHGKVAGG
                     MWNHLKSETFNVDGLGFVTNGAHVPTWIGRDVNAVLRKHVDPRFEQHLMDIDFAGGVQ
                     AIPDRELWDAHCAQKRRLVRTLRARVLSQQARHGRSPAELRALDHLLDTEVLLVGFAR
                     RFATYKRADLLLRDFSQLREIVARDERPVQFVFAGKAHPADRPGQDLIRSIWEASNNP
                     ELKGRVLFIENYDMRVARSLVQGVDVWLNNPRRPLEASGTSGMKAAFNGGLNCSVLDG
                     WWCEGYDASHGWVIGEPAGDDEGHQDHVDAESLYRVMREQVVPCFYDRDATGLPLAWV
                     ARMKRAIGLLTPRFSASRMVRQYGEQYYFPAK"
     gene            11414..12202
                     /locus_tag="VD788_14815"
     CDS             11414..12202
                     /locus_tag="VD788_14815"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HXV77582.1"
                     /translation="MRYLRLDRPLVVFDLETTGTDPAQDRIVEIAALKVAPAGDREAK
                     RRLINPERPIPPGATAVHGITDADVRDAPTFRMVARSLLDWLGDADLGGFNLLRFDLP
                     LLEREFRDCRLDLALDGRRVIDAMTVFHRKEPRDLAAAVRFYLGRELDGAHSAAADAA
                     ATLDVLEAQLERYDDLPRTVDELHAWIRAVRPHAVDVSGKFVWHEREVVFAFGKHQGK
                     TLRAVAAEAPDYLQWILGSDFPEDAKRLVGDALRGEFPGPGQST"
     gene            complement(12190..13539)
                     /locus_tag="VD788_14820"
     CDS             complement(12190..13539)
                     /locus_tag="VD788_14820"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HXV77583.1"
                     /translation="MRARSLGILSTVLVAAVVCLGDRAAQAGPQRLSPNVLIVTIDTL
                     RADHMSGYGYDRATTPNLDRLMTRGMIFDRARTIEPLTGPALVSMITSVHPHEHGASR
                     NGLRMRSGLPSLPKTLKTRGYRTAALVGNWTLRDKLSGLAEHFEAYDEVLTRRRWWGL
                     VRREADAEDLTDAAIEWLEERDRSAPFLLWVHYVEPHAPYRAHDDYRGQLGLAGAGDL
                     SPHDRYDTEIAFVDDSIGRLLAFLDDSRLRGDTMIVFASDHGESLGEHGYWGHGRNLH
                     EPTLRIPMSITWGGVLERQRIDAPAQNIDIAPTVLGLLGVDPPAEFRGFDWTPVVEGR
                     PAPRDRRTLYQAHRGAVLSKHDSDLARRAGLLEVGIIQNEIKELFRVGKNRREIYDLT
                     ADPLELQAIDVDGQAPTESLLGWMRSVYDGLTAIEEQPPEPLDEESAEILRSLGYVD"
     gene            complement(13638..14366)
                     /locus_tag="VD788_14825"
     CDS             complement(13638..14366)
                     /locus_tag="VD788_14825"
                     /inference="COORDINATES: protein motif:HMM:NF016689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HXV77584.1"
                     /translation="MADRRRALPPRLAAVAALVPRGARVADVGTDAGLLALGLLDSGR
                     ADYCVASDRAAAGLDIAARRGVPYLAAGRLHLRHGDGLDVLSRADAVDVVVVAGLGAR
                     SIERILVPDRLERLGVRRVVVQPQTDPRRVRRWLCSAGLGIVAERLARERGRDYVAIA
                     ATRGAAAARAWRPTLPDDDLLDAGPFLVASGDPLVRAHWQRELRRLDRVLDRARGGAR
                     RGAAARRRLALRVLARLSGSARSV"
     gene            complement(14359..15468)
                     /locus_tag="VD788_14830"
     CDS             complement(14359..15468)
                     /locus_tag="VD788_14830"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HXV77585.1"
                     /translation="MEPRSKRSGSWSVTVGTVIGVPIRIHVTFVILLVWYGYHASRAG
                     HDPGLAIAFLLSLFGCVVLHELGHAATGIRFGVRTREIVLYPIGGVARLENMPGGKAE
                     LLIALAGPTVNLLLAVAIELVLLSAGIVPEIPAQLTRPSQLLPYLLVVNLALFLFNLL
                     PAFPMDGGRVLRAVLSLTGDPTRATTIATRVGQVTAVVFAVVGLLAGNPFLVVIALFV
                     FLGATQEALASRQHAVLSGRRARDAMATRFDTLAPQDSLPHAAELLAGGLQQDFPVVD
                     AWNRVVGLLSREPLLRGLAELGERGAVLDVMTRDVSVAAPDDDLSRVLELMRASPGAP
                     VLVLDGERLVGLITGAKLAEVAQRFRAGAVGAPRG"
     gene            15607..17292
                     /locus_tag="VD788_14835"
     CDS             15607..17292
                     /locus_tag="VD788_14835"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXV77586.1"
                     /translation="MRLDLIILTPDHQSRRFTLDREAVTLGRAHSNDLCYADDASLSR
                     RHLRFQPTVDGWVAEDFGSKNGTLRNGSRIEGATPIRPGDRLSAGHLMITVVDPDAPA
                     PSSVVFVAGEGRESRPGATIMTSLEGLMSGEATAPTAHPRQPGGGEVSRGQAFNSPIV
                     RALVRAGRELAGHRPLKELFKLILDLSIEAVGAERGVLMTLEGSELVSRSVHGEGFRI
                     STTVRDKVLEEKTSLLVRDLTQDEAFRMQHSISEQQIQTMMAVPLQTEDRVIGLIYVD
                     SRSFVRAFTPDDLNLLTVLANVAAIRIERERLAEVERREQRRTVDLEQAAEIQRAILP
                     RGNPQVPGVDLAGHNAPCRTVGGDYFDFIPYKDGRVAVVVGDVAGKGMSAALLMSNLQ
                     ARVQLLAESPRDLAQLMSRLDQSVSINAPLNRFITLFFGLVDPVNGLLTYVNAGHNPP
                     LVIRADGSIEKLKAKGTVLGILPEIGYEEQSVGFLPGDAIAIYSDGVTEAATASGDEF
                     GEDRLARLIADNRDRSAVEIIDVILDAVSQFTGGGPPEDDVTLLIARHTGLGV"
     gene            17289..>17530
                     /locus_tag="VD788_14840"
     CDS             17289..>17530
                     /locus_tag="VD788_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77587.1"
                     /translation="MNLAPFRPARWLPGPHLQTIFAALWVGPPVDGMPELLRVAVGQG
                     STVVVAVNRPARSPAGTLLLIHGMCGSAESAYMRRTA"
BASE COUNT         2794 a         6120 c         5983 g         2633 t
ORIGIN      
        1 tgcagcgcgg gaggcaacaa caacagctga tccggatcgt acgggcggaa ggtcttgtcc
       61 atgccccgat tattccagac ccgccaatcc gcaggaactg gaaactacaa tcgaatccga
      121 ttactcggac aggctcctag ccgttgacgg gttgcagcgc tccgctctcg agccaatgcg
      181 cgtacaaccg acgcacgcgg taggtgtcga ccgggaccga ctgctcgcag gcggcgggcg
      241 agatgccctt ggccgcctgg gcccagatgc cgcgtaagaa ctcgacgtcc gattcgccct
      301 ccatcgacgt cggtttctgg ccggtcggcc tcagctcgac gtcgtcgggc gcgacggcgc
      361 gcgccatctg gaactcgtcg tagcgccgca gcgcctcgag caggccggac tggacgtcca
      421 gcatcggggg ttggtccgtc gcctgcggcg gcgggtcgct cggctggaac tcgaaggtac
      481 cgggcacggg ggtttcgaac agttggtaga acgcgtcctc gccctgcagc ctgccgttgg
      541 cgcagccctg cacgcggccg tcccggatct cgaccgtcgc gaactgctcg tcgtgctcgt
      601 tgcgcagggt caaccggccg gtcacgctcg agtcggcgag cgactgcaac aggttcggca
      661 gaccgaacag ctcgacgtcg ccggccaagc cgcgatgcgt ctcgcggcgc gatttcttct
      721 cggggagccc gctggcgtcg aagcccgaga ggatcgccgc ggcttcgcga ccgaactcct
      781 gctcgggaaa accggaggcg atccgctgca gcatggcctt gacgtcagca gtcggcgtgc
      841 cggcgagcgc gcgcgagagt tggacgatgc tgctctggtt cttcggcatg acgaagccga
      901 ggaccttcat cgggacctcg cgcttgagtg cccggagcag cagatcgacg atctccgagt
      961 cgtccgacag atcgttgccg gatagctcgg ccacgcgggc cagcgcgttg cccagcaccg
     1021 gccgtcgctg gaacgcatgc ttgacgacgg cccggttggc cgacgtgctc tgctggcgcg
     1081 ccaacccggc cgcgatccga tcgagcaggg cgcgcagctc gttggccgtg tcgatctcgg
     1141 tcttctcgcc gagggcctgc tcgacctgct cgagccgcgt gatcaacacc cgctcgacag
     1201 ccgggctctt cagctgggac aacgccccga tcgcctcgcg gaccaacagc atcgggtagt
     1261 gcagcgacga cagctcgccg tagtagccga tctcgatttc gcgcgtgtcg tcgtcagccg
     1321 gcgggatgcg acgcagcagg aagaccagat tgcgctggaa gtagccgaac gcgtcctcga
     1381 gccggccatc ggcgcaggcc tgcagccggt cgagcgcggc gcgccgcgtg gcctggccgt
     1441 gcgcttcgag cagtgccagc atcatccggc gctggtcgcg ccgctcttcg accgccactg
     1501 cgtcgagcag cccgtcgacg gtgtaggccg ggaacaggct catcacctgg ctgagcaggc
     1561 cgtgcttctc cgggcgttcg gcgaactggc gcagccgatc ggccgacagt gtgtcctggc
     1621 tccggcgcag gatgccgccg acgacctccg ggtcgagctt catccgctgc agcgattgcc
     1681 gggcgagcgc cagcatcttg gccgcctgga ccaacgcgcc gtcgttgaaa tgggtgacgg
     1741 cggcctggac gatgtcggtc gtccgttgca gtccctcgcc cgactgctcg gccatcgaca
     1801 tgatgtgctg catcgcgcgc tcggaggagc tggccggtgc cggggcatcg tcgccgacgg
     1861 tccagccggg tacggcctgg ccgagcgtgc ggaacacctc cggcatcccg ccctcgacgc
     1921 cgatctcctc gagctgttcg agatacctgg cgaactcgcg cgtgtcgcgc gagtgtgcgg
     1981 cggccgtggc gagcgcgtag gagctcatcc gcggctcgcc ggccgtggtt gcctctgcgc
     2041 gctccgcgcc gccgaccgcg cggccgaggt gacgcagcat cacggtgagc cggcgcaagc
     2101 cgcgggccag ctcgacgctc tcgccgccct cgggaagcgg cagcgacgga atcgacggca
     2161 tcgtgatcgg aggggcggcc ggcatgaccg aggccgatgg cccgctcgcg cccaccggcg
     2221 cgcccccggc gccgggcgac ggtccaccgc gggatcccgg ctgccggtgg aggataccga
     2281 cgctcgcgac cggaccggcg cgcgtgacgt cacccaggcg accgagattc tgctcgagcg
     2341 tcgcgcgctc gtgctcggga cacagcggca ggacctgctg gctgacgaga cgcaggaagg
     2401 cggtcatctt cttgtcgtcg atcagttcgt actcgatcat ctggtgcagc ttcttgagcg
     2461 cgtggaacag gtagtcgccg accgtggcgt cggagccggg tcggctgccc tgcgtcgcga
     2521 cccagccgcg gatcgcggtc accgcgaaat cgggcgggca gcgcatcagc gtctcgaccg
     2581 actccgagac catcagcgga gccagctcgt ccgagaagta gcgcgggagc tcgtccaacg
     2641 cttcgcgcac ctcggcctgg gacaccgacc cggtcacgct gtcgtttggg cccatcgcgg
     2701 ctccaccccc gtcgtgtagg cgaatatagg gaggggggag gggcgggtca aggtggcgga
     2761 agtccggtct ttcgcccgat ttccgcaatc gcgtcaccgg cttacgatcc ggaaattgct
     2821 gctaccgtcg gacggcatca cattggacgc gatggacccg ggcgtgcccc gggcccctcc
     2881 ctctcgtgga ggaacggaac gatgagacgc gaaccacgat ccggccaacg cggctcggcg
     2941 atgatcatgg cggtcttcgt gctggccctg ttggccagcg tcggcgccgc cctgctgttc
     3001 atcaccgaca acgagctgaa gatgtcgcag gccgacgtcg ccagcaagca ggtcttcttc
     3061 ttgtcggagg gcgggctcga agccggccgg cacgagatct tcctgatgaa caagaactcg
     3121 gccaacgtcc gcgacctgag cgaggagctg cagacggtct cgtccgacgg cacgatcgac
     3181 ttcgacccga cgagcctcgc gccgacctac gactcgtcgg gtcaggtcac cggcttcacc
     3241 ggcttcaacg acgacgtacc gctcaagtcg atgaccgcgc tggcggacgg ctggtacgtg
     3301 accttcatgc agaacgaccc ggccgagggc gtgtcgaccg tcgtcgacga caacgaccgt
     3361 gtgctgctga ccgcaatggc tgtgcgcaac gaccggcgcg tcgagatgac ccgcgccctc
     3421 gtcgagcgtc tcgacacctt cgccattccg ccgtcgacga tcacgatcct cgggcccgat
     3481 gccgagttca acggcggttc gagtagtgcc aagttttacg tcggcgacga tcatggtccg
     3541 cactgcccgc ccggtacctc cgggaacgtc ccgacagtcg gtgtgatcgg cggtggctcg
     3601 cttgacacgg ccgatgacgg gatgatcaag cccgacacct acgaatccgg cggcgtcacc
     3661 ggtcttccga cgatgagcga tctgacaacg gaccccgatc ttccggagct gtggaccaac
     3721 tgcgagctgc tcgtcgagct ggcacagatc attcatgcca gcgccgactt gatcggagac
     3781 acgaacacgc cgcgcgattc gctcggtgtc cccggcgcgg agaaggtcgt ctacatcaac
     3841 ggcgactacg aaatcaacgg gaactgggac ggtggtggtt tgctgttggt gacgggcgac
     3901 ctcgagtttt ccggtcagtc cggctggcag gggccgatct tcgtgatcgg cacgggtgac
     3961 ttcctgcgca gcggagccgg aaacggggag atctcgggtg gggtggtggt cgcaaacgtg
     4021 gccggtccgg accgaaccct cttcaccgct gacgattgcg cgggtcagga cggggtctcc
     4081 gggacttcgg atgacggcgt cgccgggagc cggtacgtca acgacggcgg cggtaccggc
     4141 ataaccggct actgcagcgc gtacttcacc gactggcaga gtctgcgtcc gttcaagctg
     4201 ctcagcttcc tgcaggagtg accggcgacc gccggtgtat cctgcccgcg atgcaacgac
     4261 gaccgacggt gcgcccggcg acggtcgcgg ccggcggcgc gcggcgcgac ctcgaccgcg
     4321 gcgaccgcga gctgttgcgc gtgatcgacg ccgagttcga ccgcgccgcg cgggccgccg
     4381 cgcactggct cgcgtgcgca cccggctgcg acgcctgctg ccacgggccg tttccgatca
     4441 caccgctcga cgtgagacgc ctcgtcgcgg ggctcggcag gctcgagcgc cgcgatccgc
     4501 ggcgggcggg cgcgatccgg gcgcgtgcgc aggccgccgt cgggcggatg gcggccggct
     4561 accccggcga tccggacagc ggcgcgctcg acgacgactc ggccgcgctc gaccgcttct
     4621 tcgaccttca cgcggcgctc gcgtgccccg cgctcgatcc gcggtcggga cgctgcgagt
     4681 tgtacgcctg gcgcccggtc tcctgccgca gctacggccc gcccgcgcgc tacgccggcg
     4741 acgatctccc gccgtgcgac ttgtgcttcc gcggcgcacc ggtcgaggtc gtcgagcgct
     4801 gtcgtgtcga gcccgatccc gacgggatcg agcaggcgct gatcgcgtcg ctcggagtcg
     4861 cccccgacga cgagtgggag acgctgatcg ccttcgcgct cgtgcgatag acgccctcgg
     4921 ccgcggcggg tagactgccg ggcggagggc ccgatggtca cacccaagtc cgagaaggcg
     4981 aggaaggacc gcacgacggt caaggcgatc gacggcgtag cgggcgagat cgccgcccgc
     5041 atctcgaagc tgtcgaaacc cgaggtgtcg tttcccgtgc gctcgctcgg caacgtgcgc
     5101 tacgacccca cccggggtta cttcgagatc ggacgcgaga agtcgacgcg cacgttgacg
     5161 gtcaacacgg ccaagacctt cgcccagacg ctgaagatga tggcgttgtc gaaggagctg
     5221 gtgcgcaccg acgacttcgc gaccaagcga gacgcctact accagtcgaa gaactggggc
     5281 gaggccaagt tcgacgagca gaccgaatcg gacacggtga tggacgacat cgaggcgatg
     5341 ttctccgtcc acggcgtcag ccgcgagcag ttgcgcttca ttcccgacga gcacggcggc
     5401 gccgtcgccg gcgagctgat cgtcttcgac caggacctcg agacgggcaa gaccgagcgc
     5461 atcgactgca cccgcttcgg ttccggggcc tactcgatcc cgtcgctgac cgagcacctg
     5521 acgttcgaga ccggcgccaa gttcgtgctg tgcgtcgaga ccggcggcat gttccagcga
     5581 ttgcagagcc acaagtactg gaagaaggcg cattgcatcc tggtgtcgat ggccggtgtc
     5641 ccgacgcgcg ccacgcgccg gttcgtgcgc aggctgtccg agcagtgcaa gctgccggtc
     5701 tacgccttcg tcgattgcga cccctacggc atctcgaaca tctatcgcac gctgaaggtc
     5761 gggtcgggca acgccgcgca catcaatcgc ttcttctgcg tgcccgacgc gcggtttctc
     5821 ggcgtcacgc ccgacgacat cgtccgctac gagctgccga cccatccgct gaaggaggtc
     5881 gacgtcaagc gggccaagga cgcgctcaag aacgacccgt tcttcaagcg gcacaagccc
     5941 tggcagcagg cgctcgacaa gctgctgtcg atgggcgtac gcgccgagca gcagtcgctc
     6001 gcgcgctggg ggctgaacta cgtgatcgac gagtacctgc cggccaagct ggccgcgccg
     6061 gagaagttcc tgccgtagct caggcctcga cgtcgccgcc ggggaccagc gccgcggggt
     6121 cgatgccgat cccgcgcaac gtaccgaccc aggcctcctc gggcgagccg tcgagaatcg
     6181 cggcggtatc gacgggcgcc acgatccacg agccttctcc gatctcgcgc tcgagctgcc
     6241 cgggacccca gcccgcgtag cccgagtagc agtggaagcg accccgagcc agccggcaca
     6301 gacgctcgag cgtggcgcgc gacgcgctga cgtgcagccc gtcgatcacc ggtcggcctt
     6361 ccgggtcccg gtgctcggcg ccgtacagca cgagtccctg ctcgggctgg accgggccgc
     6421 ccttgcgcac ccgcttgtcg ggatcacccg agtagtcgat cgcgtggtcg tcgcacaagt
     6481 ccttgagcaa caaagggctc tcgtggttga tcaccacgcc gatcgcgccg tcccgaccct
     6541 gctgcaacag cagcacgacg gacctgcgga agttggggtc gagcaactgg ggcgccgcga
     6601 cgagaaaccc cggggccaag cgatcgctca tgcccctatc atgcatcgaa tgaacgacgg
     6661 atgcaccgcg tgccgcgatt gaccggactc ggagaggtgt ttatagtgac cgtcgcgggt
     6721 cggcggcgta gccaagtggt aaggcagagg tctgcaaaac ctttactcac cggttcgaat
     6781 ccggtcgccg cctccaccgc tcccggcggc acgcgtcccc agtttcgctg catccgcacc
     6841 gcgcgttgac gattccgaaa tgacggccgg acacatcgtc gtgttcgtcc ggccactcgt
     6901 cgccgcacga gctcgacggt gcggttacca gatcgcattc gactctgacg aatccggcgc
     6961 gcagccgaga cgcgccgcgt cgcaatcggg cggaatcgct cgacaatcac gcgagaaaac
     7021 cgcgagtcga ccgacacccg cgcgtggcat ggagctcgca gtggtcgccc gcgtcgtcac
     7081 tggtccgcca ggagggacca ccgttgacgg gggtgaacgt catgtcgaat ggctcgctgt
     7141 gcgctcgaag cgcgctcgcc gtgctcgtcg ttgcggtcct gtccgccagt cccgcgctcg
     7201 ccggaacgat ctcactcgcg tgggacccgg tcacgcaccc ggacctcgcc ggctaccgcg
     7261 tcttctacgg cgtcaacgcg tccaacctcg atcagtcggt cgacgtcggg acgaacgccc
     7321 aggcgacgct gtccaacctc gccgactgca cgaactattt cgtcgcggtc aaggcgcgcg
     7381 gaaccgacgg ttcgctgagc ccgcagtttt ccaaccagat ctcgggttgg gcgcggccgg
     7441 tcgtcgcgtc ggtctcgccg ccgtcgatcc agcggacgac ccagatggcg ctgaccgtca
     7501 gcggcgtgaa cttcatgccg ggcgccagtg tcagcttctc cgacccggcg atcgtggtca
     7561 acggcgtcac ggtcaacggc tgcagccagc tcgtcgtcga cgtgaccgtg ccggacacgg
     7621 cggcggtggg cacgaccgac ctgaccgtgg tcaacccgga ccaggtattc ggtcagggca
     7681 gcggtgtgct gacggtgacg aacgcgctgc cgaacggtca gatcaaccag ccggcgagcg
     7741 acgtcacgat cgacgagggc gacaccgtca ccttcgccgc aaccggcacc gatcccgaca
     7801 gcgacctccc gctgggctac gcctgggact tcggtgatcc cgcgatcgcc aactcgaacc
     7861 aggaggaccc gggcgcggta cgcttcgaca acccgggcag ccatcaggtc acgttgaccg
     7921 tgaccgacgc gctcggcggc gtcgacccga cgccggcgat ggtcaccgtc aacgtcacgc
     7981 cggcgcaggc cccgtcgatc tcgaacgtgg cggtcccgac ggtcggctcg acgacggcaa
     8041 cggtgacgtg gacgaccaac gaggtcgcgg acagccgcgt gctctatcgc cgcccgggcg
     8101 acacggtctt ccagcaaacc ccgatcgaca cgacgcaggt gacgagccac tcggtccagc
     8161 tcgaaggtct gatgcccaac accgagtacg agttctcggt gcgcagcacg gacgccgacg
     8221 gtctgacgac gactcagccc gccgcgacga ccttcaccac gcagccgaac tcgttcactt
     8281 atctccgtat cgaggccgaa tcgggtccga tcgccgcacc ggcgcagacc caaaacggtg
     8341 tcgacgcctt cgccggcgtc tacgtcaggc tggctccggg gaccgcctcc ggcaccgcgg
     8401 gcagcccggc gggcacgtgg gactacggat tcaacctcac gtcaccggcg acctactact
     8461 tctggtaccg cctgtacgcg cccagcagcg gcagctcgcg ctggttcgag tcggtcgatg
     8521 gcggcagctt cgccgcgatc gctcccccga ccaccgcctc gtgggagtgg gtcgagggcc
     8581 gctcgttcgg tctggcggcc ggccagcacc gggtcacgtt gggcggcggc gacgctcagg
     8641 cgcgggtcga ccgcattctg atcacgaacg acgccggctt ccgtccgacc gagggcccgg
     8701 gcggcgacgt cagcccgccg gcgccggtca acgcgctgaa cgcggcgccg gacgacacga
     8761 cggtggcgct gagttggacc aacccgaccg acagcggacc gctgcgggtc gtcgtccgct
     8821 tcaccaccgg cccgagccac ccggccaacc cgcaggacgg ccagccgctg atcgaccgcg
     8881 ccgcccccgc cggtgcgagc gacggcgtgc tgcacaccgg actggcgaac ggcacgacct
     8941 accgctacag cgtgttcgtc gtcgaccagt ggggcaacgt ttcgagccgg gcgacgacca
     9001 gcggcacgcc ggtagcggcg gtgataccgc tcgatccggt gaccaacctg cgccggaccg
     9061 acacgttatc gccctgagcc gtcggcggtc ggtcggcgat cgagcccccc ggcgcgagcc
     9121 ggggggcttt tcacttggcc gggaagtagt actgctcgcc gtactggcgc accatccgtg
     9181 aggcggagaa gcgcggcgtc agcagcccga tcgcacgctt catccgcgcg acccaggcca
     9241 gcggcagacc cgtcgcgtcg cgatcgtaga agcacggcac gacctgctcg cgcatcaccc
     9301 ggtacagcga ctccgcatcg acgtggtcct ggtgcccctc gtcgtcgccg gccggttcgc
     9361 cgatgaccca gccgtggctc gcgtcgtagc cctcgcacca ccagccgtcg agcacgctgc
     9421 aattgagtcc gccgttgaac gccgccttca tcccgctcgt cccgctggcc tcgagcggcc
     9481 gccgcgggtt gttcaaccac acgtcgaccc cctgcaccag gctgcgcgcc acccgcatgt
     9541 cgtaattctc gatgaacagc acgcgcccct tcagctccgg gttgttcgac gcttcccaga
     9601 tgctgcggat cagatcctgc cccgggcgat cggccgggtg cgccttgccg gcgaagacga
     9661 actggaccgg tcgttcgtcg cgcgcgacga tttcgcgcaa ctggctgaag tcgcgcagca
     9721 acaggtcggc gcgcttgtag gtggcgaacc gcctggcgaa cccgaccaac aggacctcgg
     9781 tgtcgagcag gtgatcgagc gcccgcagct cggcgggcga ccggccgtga cgcgcctgct
     9841 ggctgagcac gcgggcacgc aacgtacgca ccaatcgccg cttctgcgcg cagtgcgcgt
     9901 cccacaactc gcgatcgggg atcgcttgga ccccgcccgc gaagtcgatg tccatcaggt
     9961 gctgttcgaa ccgcggatcg acgtgcttgc gcagcacggc gttgacgtcg cgaccgatcc
    10021 aggtcggcac gtgggcgccg ttggtgacga accccagccc gtcgacgttg aacgtctcgc
    10081 tcttgaggtg gttccacatc ccgcccgcga ccttgccgtg cagctcgctc accccgttgg
    10141 cgcggcggct ggtccggatc gcgagcgcgg tcatgttgaa cgactcgtct ccgccgtcgt
    10201 cgcgcgcgcg cccgagcgcg agcagggaat cgacgggaac tcgcccgggc gcggcccacg
    10261 attcgacgta ctggcgcacg agctcccggt cgaaggtctc gttgccggcc ggcaccggcg
    10321 tatgcgtcgt gaacaccgcg ttctcgttga cgcgcagccg cgcctcgtcc cacgacaggc
    10381 cgtcgacctc ggtcagacga cgcagccgct ggaacgacag caacgcggag tggccttcgt
    10441 tcatgtgcca caccgccggg cggatcccga gtgcgcggac gaactcgacg ccgccgacgc
    10501 cgaggaccca ctcctggcac agtcgcatct cgcgcccctg cacgtagagc acggaggtga
    10561 tcggccggtc ggcgggatgg ttggcggcga gatcggtgtc gagcagcagc acctggacgc
    10621 gcccgacgct cgctttccac gcgcggagct gcacgtcgcg accggccagc tcgatcgtca
    10681 cccgcagctc gccgttctgc tcgtcgacga ccggaagcaa cggcacgcgg tgaaagtcgt
    10741 agtccggata gaaatgctgc tgctggccgt cggcgtcgat cgtctggcgg aaatatccgt
    10801 gctggtacat caggccgatg ccgatgaacg gcaggtcgag atcgctcgcc gccttcgagt
    10861 gatcgcccga cagcacgccg agtccgccgg agtacatctg caggcactcg tgccagccgt
    10921 actccgtcga gaagtacgcg accggcccgc cgtcgtagcc cggaaagcgc gagtcgaacc
    10981 aggtcggctc gtccggcgcc atgtagaggt cgaactcttc gacgatggcg cggtaggtcc
    11041 gaatgaagtc ccggtcgcgc tgcagctcgt gccagcgctg cggctcgagg tcgatcagca
    11101 gctcgatcgg gttgcggtag tgacgccagc gggcgttgtc gatcatgtcg aacagcgctc
    11161 gggtcggttg actccaggcc cagaacaggt tgtacgcgat gtccatcaat cgcgagaact
    11221 ggaccgggat ctcgatactc gccgtcggtt tggtcttgat ttcgcgtggc tgcattcgtc
    11281 tcctcgccgc atccgagcgc ataatctacc gaacttcacc cgacggaacc aaccggggct
    11341 tgcgtggtcg aggccgagcg attagtgtcg cctggcgccg tccgggggcc ggacgcgggg
    11401 gagggggcgc aaggtgaggt acttacgact cgaccgcccg ctcgtcgtct tcgacctcga
    11461 gacgaccggt accgacccgg cccaggaccg catcgtcgag atcgccgcgc tgaaggtggc
    11521 tccggcgggc gatcgcgaag ccaaacggcg actgatcaac cccgaacgtc cgatcccgcc
    11581 cggggcgacg gccgtccacg gcatcaccga cgccgacgtg cgcgacgcgc cgaccttccg
    11641 catggttgcc cgcagcctgc tcgactggct gggagacgcc gacctcggcg gcttcaacct
    11701 gctgcgcttc gatcttccgc tgctcgagcg cgagttccgc gattgccggc tggacctggc
    11761 gctcgacgga cggcgggtga tcgacgcgat gacggtgttc caccgcaagg agccgcgcga
    11821 tctcgccgcg gccgtccggt tctacctcgg ccgcgagctc gacggggcgc actcggccgc
    11881 ggccgatgcc gcggccacgc tcgacgtgct ggaggcgcag ctcgagcgtt acgacgatct
    11941 gccgcgaacg gtcgacgagc tgcacgcgtg gatccgcgcg gtgcggcccc acgcggtcga
    12001 cgtcagcgga aagttcgtct ggcacgagcg cgaggtggtc ttcgccttcg gcaagcacca
    12061 gggcaagacg ctgcgcgccg tcgccgccga ggcgccggac tacctgcagt ggatcctcgg
    12121 ctccgacttt cccgaagacg cgaagcggct ggtgggcgac gcgctacgcg gtgagtttcc
    12181 cggccccggt cagtcgacgt agcccagcga acggaggatc tcggcgcttt cctcgtccag
    12241 cggctcgggc ggttgctctt cgatcgcggt cagcccgtcg tagaccgagc gcatccagcc
    12301 caagagcgat tccgtcggcg cctggccgtc gacgtcgatc gcttgaagct cgagcggatc
    12361 cgcggtcagg tcgtagatct cccggcggtt cttgccgacg cggaacagct ctttgatttc
    12421 gttctggatg atgccgactt ccaacagccc ggcgcgccgc gcgagatcgg agtcgtgctt
    12481 ggacagcacc gcaccgcggt gcgcctgata cagcgtgcgt cggtcgcgcg gggcgggtcg
    12541 gccctcgacc accggggtcc aatcgaagcc gcggaactcg gccggtgggt cgacgccgag
    12601 caggccgaga accgtcggcg cgatgtcgat gttctgcgcc ggggcgtcga tgcgttggcg
    12661 ttcgagcacg ccaccccagg tgatcgacat cgggatgcgc agcgtcggct cgtgcaggtt
    12721 ccgcccgtgg ccccagtaac cgtgttcgcc gagactctcg ccgtggtcgg aggcgaaaac
    12781 gatcatcgtg tcgccgcgga gccggctgtc gtcgaggaac gcgagcaggc gcccgatcga
    12841 gtcgtcgacg aacgcgatct ccgtgtcgta acgatcgtgc ggcgacaggt cacccgcccc
    12901 ggcgagcccg agctgcccgc gatagtcgtc gtgggcccga tacggggcgt gcggctcgac
    12961 gtagtgcacc cacaacagga acggcgccga ccggtcgcgt tcctcgagcc attcgatcgc
    13021 cgcgtcggtc aggtcctcgg catccgcctc gcgacgcacc agaccccacc agcgacgccg
    13081 cgtcagcacc tcgtcgtagg cttcgaagtg ctcggcgagc cccgacagct tgtcccgcag
    13141 cgtccaattg ccgacaagtg ctgcggtgcg gtacccgcgc gtcttcaatg tcttgggcag
    13201 cgagggcaaa ccgctgcgca tccgcagccc gttgcggctc gcgccgtgct cgtgggggtg
    13261 caccgaggta atcatcgaga ccagcgcggg gccggtcagc ggttcgatgg tgcgcgcccg
    13321 gtcgaagatc atcccgcggg tcatcaaccg gtcgagattc ggtgtcgtcg cccgatcgta
    13381 gccgtagccc gacatgtggt cggcacgtag cgtgtcgatc gtgacgatca agacgttggg
    13441 ggaaaggcgc tgcggcccgg cctgcgccgc ccgatcgccg aggcagacga ccgctgccac
    13501 cagcaccgtc gagaggattc cgagggacct cgctctcacc gctcgctcgc tctccgatcg
    13561 gctcgactgc tcggaacgtc agagcggcgg gcgatcggag catccccgac cgtctcgcgc
    13621 cgcgaagtcg cagtatatca gacggatcgc gcgctgcccg acagccgggc caacacccgg
    13681 agcgcgagcc gccggcgcgc cgccgcgccc cggcgcgcgc cgccccgcgc acggtcgagc
    13741 acccggtcga gacggcgcag ctcacgctgc cagtgcgctc ggacgagcgg atcgcccgag
    13801 gcgacgagga acgggccggc atcgagcagg tcgtcgtcgg gcagcgtggg gcgccacgcc
    13861 ctcgcggcag ccgccccgcg cgtggcggcg atcgcgacgt agtcacgacc gcgctcgcgg
    13921 gccagccgct cggccacgat gccgagaccc gcggagcaca accagcggcg gacccgtcgc
    13981 ggatcggttt gcggttggac gacgacgcgg cgcacaccca gacgctcgag ccggtcggga
    14041 acgaggatgc gctcgatcga tcgcgcgccg agaccggcga cgacgacgac gtcgacggcg
    14101 tccgcacgcg acagcacgtc gagcccgtcg ccgtgccgca ggtgcaggcg cccggcggcg
    14161 aggtagggca cgccccgtcg tgcagcgata tcgagacccg cggcggcgcg gtcgctggcg
    14221 acgcagtagt ccgcccggcc gctgtcgagc aggccgagcg ccagcagtcc ggcgtcggta
    14281 cccacatcgg ccacgcgagc gccgcgtggc acgagcgccg cgacggcggc caggcgcggc
    14341 ggaagggccc ggcggcggtc agccacgcgg cgcaccgacc gcgcccgcgc ggaagcgctg
    14401 cgcgacctcg gccagcttgg cacccgtgat cagaccgacc aggcgctcgc cgtcgagaac
    14461 caggaccggc gcgcccggac tggcccgcat cagctcgagc acacgcgaca ggtcgtcatc
    14521 gggcgcggcg acgctgacgt cgcgcgtcat cacgtcgagg acggcaccgc gttcgcccag
    14581 ctcggccagg ccgcgcagca gcggctcgcg cgacagcagg ccgacgacgc ggttccaggc
    14641 atcgacgacc gggaagtcct gctgcagccc cccggccagc agctcggcgg cgtggggcag
    14701 cgaatcctgc ggggccaagg tgtcgaatcg cgtcgccatc gcgtcgcgcg cccgacgtcc
    14761 gctgagcacg gcgtgctggc gggaggcgag cgcctcctgc gttgcgccga ggaagacgaa
    14821 gagcgcgatc acgaccagga acggattccc cgcgagcagc ccgacgaccg cgaagacgac
    14881 ggcagtcacc tgaccgacgc gtgtcgcgat cgtcgtcgcc ctcgtggggt ccccggtcaa
    14941 gctcagcacg gcccgcagca cccgaccccc gtccatcgga aacgccggga gcagattgaa
    15001 caggaacagg gcgaggttga ccaccaacag gtacggcagc aactgggacg gtcgagtcaa
    15061 ctgcgccggg atctccggca cgataccggc cgagagcagc acgagttcga tcgcgaccgc
    15121 cagcagcagg ttgacggtcg gtccggccag cgcgatcagc agctcggcct tgccgcccgg
    15181 catgttctcg agacgtgcca cgccgccgat cggatagagc acgatctcgc gcgtcctgac
    15241 accgaaacgg atcccggtcg ccgcgtgccc cagctcgtgc agtacgacgc aaccgaacaa
    15301 cgacagcagg aacgcgatcg ccaacccggg atcgtggccg gcgcgcgagg cgtggtaccc
    15361 gtaccaaacg agcaagatga cgaacgtgac gtggatccgg atcgggacac cgatcacggt
    15421 gccgacggtc accgaccagc tcccgctgcg tttgctccta ggttccatcg tcgaaccccc
    15481 ggacgccagg ttcgcggtgc ccgccgtgct attctagcgt ccaccgcgtg aaggtgacga
    15541 accgcctgat ccggcgaacc aattgttctt caaccggtcg gcgcgttgcg cgagggcacc
    15601 acggacgtgc gcctggatct catcattctg accccggatc accagagccg gcggttcacg
    15661 ctcgaccgcg aggcggtgac gctcggccgg gcccacagca acgacctgtg ctacgccgat
    15721 gacgccagcc tgtcccggcg ccatttgagg ttccagccga cggtcgacgg ttgggtcgcc
    15781 gaggacttcg gcagcaagaa cggcacgctg cgtaacggct cgcgcatcga aggcgcgacg
    15841 ccgatcagac ccggtgaccg gctgagcgca gggcacctga tgatcacggt cgtcgacccc
    15901 gacgcgccgg ccccgtcgag cgtggtcttc gtcgccggcg aggggcgcga gagccgcccc
    15961 ggcgccacga tcatgaccag cctcgagggt ctgatgtcgg gtgaggcgac cgcaccgacg
    16021 gcccatccgc ggcagccggg cggcggcgag gtcagtcgcg gccaggcgtt caactcgccg
    16081 atcgttcgcg cgctggtacg cgccgggcgc gagttggccg gtcatcgtcc gctgaaggag
    16141 ctgttcaagc tgatcctcga cctgtcgatc gaagccgtcg gcgccgagcg cggcgtgttg
    16201 atgacgctcg agggaagcga gctggtgtcg cggtcggtcc acggtgaagg cttccgcatc
    16261 agcacgaccg tgcgtgacaa ggtgctcgag gagaagacgt cgttgctcgt gcgtgacctg
    16321 acccaggacg aagcattccg catgcagcac agcatcagcg agcaacagat ccaaacgatg
    16381 atggccgtgc cgttgcagac cgaagaccgg gtgatcgggc tgatctacgt cgactcccgg
    16441 tcgttcgtcc gcgcgttcac acccgacgat ctcaatctgc tgacggtgct ggccaacgtg
    16501 gcggcgatcc gcatcgagcg cgaacggctg gccgaggtcg agcgccgcga gcagcggcgg
    16561 acggtcgacc tcgagcaggc ggccgagatc cagcgggcga tcctgccgcg cgggaatcca
    16621 caggtcccgg gggtcgacct tgccgggcac aacgccccgt gccgcacggt cgggggcgac
    16681 tacttcgact tcatcccgta caaggacggg cgcgtcgcgg tcgtcgtcgg cgacgtcgcc
    16741 ggcaagggga tgtcggcggc gctgctgatg agcaatctgc aggcccgcgt gcagctgctg
    16801 gccgagtcgc cgagagatct ggcgcagctg atgtcgcgac tcgatcagag cgtgtcgatc
    16861 aacgcgccgc tcaatcgatt cattacgctg ttcttcgggc tggtcgatcc ggtcaacggt
    16921 ctgttgacct acgtcaacgc cggtcacaac ccgccgctcg tgatccgcgc cgacggctcg
    16981 atcgagaagc tgaaggccaa gggaacggtg ctcggcatcc tgcccgagat cggctacgag
    17041 gagcagtccg tcggcttcct gccgggcgac gcgatcgcga tctacagcga cggcgtgacc
    17101 gaggcggcga cggcgtcggg cgacgagttc ggcgaagacc ggctggcccg gttgatcgcc
    17161 gacaaccgcg accggtcggc ggtcgagatc atcgacgtga tcctcgacgc cgtttcgcag
    17221 ttcaccggcg gtgggccgcc cgaagacgac gtgacgttgc tgatcgctcg ccatacgggg
    17281 ctcggcgtgt gaacctggcc ccgttccggc cggcgcgctg gctcccgggg ccgcacctgc
    17341 agacgatctt cgccgcgctg tgggtcggtc cgccggtcga cggcatgccc gagctgttgc
    17401 gcgtcgcggt cgggcaaggt tcgacggtgg tggtcgcggt caaccgtcct gcgcggtcgc
    17461 ccgccgggac gctgctgttg atccacggca tgtgcggctc ggccgaatcg gcatacatgc
    17521 ggcgcaccgc
//
LOCUS       DASPGN010000512         4915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_330703, whole genome shotgun sequence.
ACCESSION   DASPGN010000512 DASPGN010000000
VERSION     DASPGN010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330703"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..709
                     /locus_tag="VD788_14845"
     CDS             161..709
                     /locus_tag="VD788_14845"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wss1p-related putative metallopeptidase"
                     /protein_id="HXV77588.1"
                     /translation="MTRRRPGPEPPALFEPEELTLRPTPHARLRKLAARASARGYPAT
                     RFEEDRETIDRLRSWGAELAEHFQLRVRSIDVERDGVVRHYGICYDDGTIRIRLRHAR
                     SGKLLKESSLVDTLCHELAHLKHLDHSIRFRRLYVRILEQAREWGYYRPGPGETRRPR
                     QLALFERGTCGTARHATRGEGP"
     gene            706..1974
                     /gene="hemL"
                     /locus_tag="VD788_14850"
     CDS             706..1974
                     /gene="hemL"
                     /locus_tag="VD788_14850"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HXV77589.1"
                     /translation="MNDLFEQAKRYIPGGVNSPVRAFRSVGGTPIFFHSADGPRVRDV
                     EGREYIDYVLSWGPMIVGHAHPEIVAAVQQQAALGTSFGAPCQAETDLARMICERVPS
                     CELVRFVSSGTEATMSAIRLARGASGRDEVIKVEGCYHGHVDSLLVRAGSGVMTLGIP
                     GSPGVPTALAELTHVVPFNDADAVERLLDRRGDRIACMIVEPIAGNMGTIPPADGYLV
                     RLRELTAQHGVVLIFDEVMTGFRVDRSCAQGLYGVKPDLSTFGKVIGGGLPAAAYGGT
                     RELMEQVAPTGSVYQAGTLSGNPLAMRAGIETLKLLDQPDAYRSLEATSADLERGLRD
                     AARRSGQAVVINRVGSMMTAFFTDREVVDYTTAAQSDTDKYARYFHGMLDRGVYLAPS
                     QFEATFVSLAHGEAEIAATVRAAEITLKQL"
     gene            1999..2685
                     /locus_tag="VD788_14855"
     CDS             1999..2685
                     /locus_tag="VD788_14855"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXV77590.1"
                     /translation="MLKLGFHGHSCWELDDGKKRVLIDPFLTGNPLARVGPDHFDKLD
                     AIIVTHGHGDHLGDAVRIARASGALVVSNFEIATYVGARDCQAHPMHIGGGRKFPFGH
                     VKFTIAHHGSTGPEGEALGNPMGVVITMGGKKVYHAGDTGLFLDMKLIGEMYGPLDVA
                     LLPIGDNFTMGIDDAVKACEFLRAKITIPMHYDTFEPIKADPNEFVAKVADRGGRALV
                     INPGQVYELG"
     gene            complement(2700..>4915)
                     /locus_tag="VD788_14860"
     CDS             complement(2700..>4915)
                     /locus_tag="VD788_14860"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXV77591.1"
                     /translation="SGLNPAQRQAVIHGGGPLLVLAGAGSGKTRVIVHRIAHLILVQG
                     VPADQIVAVTFTNKAASEMRERVQRLLGAGAIGSWIGTFHGLCLRILRRDGHHIGLAP
                     GFNIYDTDDQLALLKQILRERDDTDDLGTPRSMLARISRAKNGMQSADDLAKTAFSPE
                     RKRLVEVYREYDAGLRRAQAVDFDDLLLRTLELLRTQADVAERYASRCGQLLVDEYQD
                     TNRPQYLLIRRLSAAHGNVTVVGDEDQSIYRFRGAEIRNILDFERDYPGTCTIRLERN
                     YRSTGTIIEAAGAVIARNVQRKGKALWTENPRGSAIELYRAPDDRIEALWVAQRVRQL
                     ESDHALYEMAVLYRTNAQSRQFEEVFRREKIAHQVVGSVRFYERREVKDLLAYLKLAV
                     NASDDVAFTRVVNTPPRGVGATSLATIAGVARARGVPLIRAASVAIDDSLIAPRAAAS
                     LRAFLELIDDLSRRAAEDEVAGLVERVVEAVSYEAYLHRVFPGQGRERMENVRALVSA
                     TVEYIEEDDRATLHGFLDRLALVSDADEVGSGGGVTLMTIHCAKGLEYPVVLLAGMEE
                     NLFPHVMATQDPDDVEEERRLFYVAMTRAKERLLLSHARFRRTQGVQLVARASRFLDE
                     LPPELIEESSAPPDDVLDVQAPFAAGDSGPGSSAARVAARLEPRERRAPPVARGADPG
                     DGYPVGAWVQHPRFGAGRILQREGRGTNLKLTIHFADYGPKRIAPSYTQLRVQLD"
BASE COUNT          794 a         1645 c         1669 g          807 t
ORIGIN      
        1 cctacaacgt cagcggcgag ttttcgatgc tcgaggcggc gttcgcgcgc ggctggctcg
       61 agcgcgaccg cacgattctc gaatcgctca cctcgatcaa gcgcgcgggc gccgacatca
      121 tcctgactta tcatgcccgc gaggcggcgc gccttctctc atgacgaggc ggcggccggg
      181 ccccgaaccg ccggcgctgt tcgagccgga ggaactgacc ctgcggccga cgcctcacgc
      241 ccgtctgaga aagctggccg ccagggcgtc cgcgcgcggc taccccgcca cccgcttcga
      301 ggaagaccgt gagacgatcg accgcttgcg gagctggggc gccgaactgg ccgagcattt
      361 ccaactgcgt gtccgctcga tcgacgtgga gcgggacggc gtggtgcggc actacggcat
      421 ttgctacgac gacgggacga ttcgtatccg gttgcgtcac gctcggtcgg gtaagctgct
      481 gaaggaatcc agcctggtgg ataccctgtg tcacgagctg gcgcacctga agcacctcga
      541 tcactcgatc cgatttcgcc ggttgtacgt caggatcctg gaacaggcgc gcgaatgggg
      601 ctactaccgg ccgggtccgg gcgagacgcg tcggccgcgc cagctcgcgc tgttcgaacg
      661 cggcacctgc ggcacggcgc ggcacgccac gcggggggag ggtccgtgaa cgatctgttc
      721 gagcaggcca agcgctacat cccgggtggc gtcaacagcc cggtgcgagc gttccggtcg
      781 gtgggaggca cgccgatctt cttccacagc gccgacgggc cgcgtgtccg tgacgtcgag
      841 ggtcgcgagt acatcgacta cgtcttgtcc tgggggccga tgatcgtggg ccacgcccat
      901 cccgagatcg tcgccgcggt tcagcagcag gccgcgctgg gcacttcgtt cggggcacca
      961 tgccaagccg agaccgacct ggcgcggatg atctgcgagc gggttccgag ttgcgagttg
     1021 gtgcgcttcg tctcgtcggg taccgaggcg acgatgtcgg cgatccggct ggcgcgcgga
     1081 gccagcggac gcgacgaggt gatcaaggtc gagggttgct accacggcca tgtcgactcg
     1141 ctattggttc gtgccggctc gggcgtgatg acgctgggta tccccggcag ccccggcgtg
     1201 ccgacggcgc tcgccgagct gactcacgtc gtgccgttca acgacgccga tgcggtcgag
     1261 cgcctgctcg accggcgcgg cgaccgaatc gcctgcatga tcgtcgaacc gatcgccggc
     1321 aacatgggga cgatcccgcc ggccgatggc tacctcgtgc gactgcggga gttgaccgcc
     1381 cagcacggcg tcgtgttgat cttcgacgag gtgatgaccg gcttccgcgt cgaccgctcc
     1441 tgcgcccagg ggttgtacgg ggtcaaaccc gacctgtcga cgttcggcaa ggtgatcggc
     1501 ggcgggctgc cggcggcggc ctacggcggc acgcgcgagt tgatggagca ggttgcaccg
     1561 accggttcgg tctaccaggc cggtacgctc tcggggaacc cgctggcaat gcgcgccggg
     1621 atcgagacgc tcaagctctt ggatcagccc gacgcctacc ggtcgctcga agccacctcg
     1681 gccgatctcg agcgtgggct gcgtgatgcc gcgcggcgct cgggacaggc ggttgtgatc
     1741 aatcgcgtcg gctcgatgat gacggcgttc ttcaccgatc gggaggtcgt cgactacacg
     1801 accgccgcgc agtccgatac cgacaagtac gccaggtatt tccacggcat gctcgatcga
     1861 ggggtctacc tcgcgccgtc gcagttcgag gccacgttcg tatcactggc ccacggcgag
     1921 gcggagatcg ccgcgaccgt gcgtgccgcg gagatcacgt tgaagcaact ctgagatcag
     1981 gcaatggcgg aggaagcgat gctgaagctg ggattccacg gacactcttg ctgggagctg
     2041 gacgacggga agaagcgcgt gctgatcgac ccgttcctca ccggcaaccc gctggcccga
     2101 gtcgggccgg accacttcga caagctggat gcgatcatcg tgacccacgg tcacggcgat
     2161 cacctcggcg acgccgtgcg catcgctcgc gcgagcggcg ccctcgtcgt gtcgaacttc
     2221 gagatcgcca cctacgtcgg tgcgcgtgat tgccaggcgc acccgatgca catcggcggg
     2281 ggccgcaagt tccccttcgg ccacgtcaag ttcacgatcg cccaccacgg ctcgaccggc
     2341 ccggagggag aagcgttggg aaacccgatg ggtgtcgtga tcacgatggg cggcaagaag
     2401 gtctatcacg ccggtgacac cgggctgttc ctcgacatga agctgatcgg cgagatgtac
     2461 ggaccgctgg acgtcgcgct gctgccgatc ggtgacaact tcacgatggg tatcgacgac
     2521 gccgtcaagg cctgcgagtt cctgcgcgcc aaaatcacga tcccgatgca ctatgacacg
     2581 ttcgagccga tcaaggccga ccccaacgaa ttcgtcgcga aggtagcgga ccgcggcggc
     2641 cgcgcgctgg tgatcaaccc gggccaggtc tacgagctcg gctgagctct cggtctgtgt
     2701 caatcgagct ggacccgcaa ctgagtgtag gacggagcga ttcgcttcgg tccgtagtcg
     2761 gcgaagtgaa tggtcagttt gagattggtg ccgcgccctt cgcgctggag aatgcgcccc
     2821 gcgccgaacc ggggatgctg gacccaggcg ccgaccgggt agccgtcgcc ggggtccgcg
     2881 ccacgagcga cgggcggcgc ccgcctctcg cgcggctcga gccgggccgc gacccgcgcc
     2941 gccgacgagc ccggtccgct gtcgcccgcg gcgaacggcg cctgcacgtc gagcacatcg
     3001 tccgggggtg cggagctctc ctcgatcagc tcgggcggca gctcgtcgag aaaacgcgag
     3061 gcccgcgcca ccagctgcac gccctgggtc cgccggaacc gcgcgtgcga cagcagcagc
     3121 cgttccttgg cgcgggtcat cgccacgtag aacagccggc gctcttcttc gacgtcgtcc
     3181 ggatcctgcg tcgccatcac gtgcgggaac aggttctctt ccattccggc gagcagcacc
     3241 accggatact cgagcccctt ggcgcagtgg atcgtcatca gcgtcacccc cccgccgctg
     3301 ccgacctcat cggcgtccga tacgagcgcc agccgatcga ggaagccatg cagggtcgcg
     3361 cgatcgtcct cctcgatgta ttcgacggtc gccgacacga gcgcgcgaac gttctccatt
     3421 cgctcgcgac cctggcccgg aaagaccctg tgcaggtagg cctcgtagct taccgcctcg
     3481 accacgcgtt cgacgagccc ggcaacctcg tcctccgcgg cccgtcgcga caggtcgtcg
     3541 atcagctcga ggaaggcgcg cagggacgcg gccgcgcgcg gcgcgatcaa cgagtcgtcg
     3601 atcgcgaccg acgcggcgcg gatcagcgga acaccgcggg cgcgtgcgac cccggcgatc
     3661 gtcgcgagcg acgtcgcacc gaccccgcgc ggcggcgtgt tgaccacccg cgtaaacgcc
     3721 acatcgtcgg acgcgttgac cgcgagcttg aggtacgcca gcaggtcctt gacttccctg
     3781 cgctcgtaga acctgaccga gcccacgacc tggtgcgcga tcttctcgcg tcggaagacc
     3841 tcttcgaact gccgtgactg ggcgttggtg cggtacaata ccgccatctc gtacaacgca
     3901 tggtcggatt cgagctggcg aacgcgttgc gcgacccaca acgcctcgat ccggtcgtcc
     3961 ggggcgcggt agagctcgat cgcggaacca cgcgggttct ccgtccatag cgccttccct
     4021 ttgcgttgga cgttgcgcgc gatcaccgca ccggccgcct cgatgatcgt cccggtcgag
     4081 cggtagttgc gctcgagccg aatggtgcac gtgcccggat agtcgcgctc gaagtcgagg
     4141 atgttgcgga tctcggcccc gcggaaacgg tagatgctct ggtcttcgtc gccgacgacg
     4201 gtgacgttgc cgtgggcggc agacaagcgg cggatcagca agtactgcgg tcgattggtg
     4261 tcctgatact cgtcgaccag caactgtccg cagcgcgacg cgtagcgctc cgcgacgtcg
     4321 gcctgggtcc gcaacagctc gagcgttcgc agcaacaagt cgtcgaagtc gacggcttgg
     4381 gcccggcgca agccggcgtc gtactcgcgg taaacctcga cgagccgctt gcgttcgggc
     4441 gagaaggcgg tcttcgccag atcgtcggcg gactgcatcc cgttcttggc ccgcgagatt
     4501 cgcgccagca tcgaacgcgg cgtaccgagg tcgtccgtgt cgtcgcgctc gcgcaagatc
     4561 tgcttgagca gagccagttg gtcatcggtg tcgtagatgt tgaacccggg cgccaggcca
     4621 atgtggtgcc cgtcacgacg caggatccgt aggcacaagc cgtggaacgt gccgatccag
     4681 cttccgatcg caccggcgcc gagcagacgc tggacacgct cgcgcatctc ggacgccgct
     4741 ttgttggtga aggtgacggc gacgatctgg tcggccggca caccttgcac caggatcaag
     4801 tgggcgatgc ggtggacgat gacgcgcgtc ttgcccgatc ctgcgccggc cagcaccagc
     4861 aacgggccgc cgccgtggat cacggcttga cgttgcgccg ggttcagtcc cgaca
//
LOCUS       DASPGN010000513         7177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1265982, whole genome shotgun sequence.
ACCESSION   DASPGN010000513 DASPGN010000000
VERSION     DASPGN010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1265982"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="VD788_14865"
     CDS             <1..1199
                     /locus_tag="VD788_14865"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXV77592.1"
                     /translation="NPATDETRAITRDFDPAVVDAKWSAGDGRIYLLAEERDTRPLFR
                     FDPETDSFERLDTGYDVNRELALARNAPTVVLLASSPWVAERLVAVDGARDAARTLEH
                     PAGEWLAGVSRGTVEPWTFVAANGKTIDGRVYVPPDFDAGNRYPAIVYYYGGTSPVGR
                     EFGGRYPKEWWAAQGYVVYVPQPSGATGFGQEFSAAHVNDWGKTTSEEIIEGTRRFLE
                     AHPFVDAGRVGCIGASYGGFMTMLLSTKTDLFAAAVSHAGISSLASYWGEGYWGFLYS
                     SVATAESYPWNRKDIYVDQSPLYRADENRVPILLTHGVADTNVPVGESDAFYVALELL
                     GKEVEYLQIEGENHWIVDHAKRVVWSRSIVAWFDRWLKDEPQWWQALYPPGGPPAPDE
                     KSKTAR"
     gene            complement(1260..1871)
                     /locus_tag="VD788_14870"
     CDS             complement(1260..1871)
                     /locus_tag="VD788_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77593.1"
                     /translation="MNRQPLLYRTLCLGAVLLTGTLASACGGGGSSGGGGGGMGNLVA
                     TFVAGNPAPGANTISLAPGGSNGASFSVVVQITGRNDLLSAAFHVTYDPAVAEFVGFS
                     SAGSFLTSNGDDLFFDAELDQGMPGEVKVIAAITDERPPGVDFVGTQTLITLNFMALT
                     TAQNSAFGFAGPDDTEFHICPTQDGECTTTNMNQSGGFLNATQ"
     gene            complement(1883..4042)
                     /locus_tag="VD788_14875"
     CDS             complement(1883..4042)
                     /locus_tag="VD788_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77594.1"
                     /translation="MNSKRILGTWIVAVAATVIPAQAQIFVDTQNGSCTDAGSGLPAA
                     PFCTIQAAYDFAADGAEIRVLPGTYTECLFLFDLDTQKSIEVVANAWLTSTDNSVTII
                     DGSTVPSVDCEFATSLAPASVVNIGGTASRFTGFTVTGGTASGIFGVGSVEITDNVVT
                     GNTSTIGGGIYVYSGNCYYGDTVMTISDNQVTGNSVTLQGGGMSIAAGLSVNAQNPDP
                     ITCPFDGDATITINNNTIDGNTSESNGGGIYATVFTNDQRQATVTITSNTISDNTSST
                     PGQSGTIGYGAGLYGLTYGYGTERIEVEDNTITGNSTLDFGGGAQLTVFPTVIASSID
                     HQYHVSDNQISGNRAGRGGGGMELFVSVLDMNNPQAFSMIAENNTLTNNVAGVVGTTT
                     IDDLDESFGGGGLLLTMESLSSDSPNMEFSVRNNTITGNSAKLLGGGVSMFVEADALP
                     PGAVSVAPADAFLDFRNNRVTGNSTDSNVRNAVGGGVFALLTARGQDAVADVDATWNT
                     ISSNTLDNQAEPGGFHLESLTSADPLSATDGQAALTLDSSIVSGNNGVGFGGPSPGTA
                     GVKTVGGTAEFSVVTTYNDLFGHPQGNLSGWVVSGTGNITTDPLLDASFLPGLCSPTI
                     DAANPALPFAPEPAPNGGRANMGFTGGTAAAQRSLADVSGDGEVDGIDVGRLAVAFGA
                     QTGVHVRYNAAVDLDQNGMVDGVDLAVIAADFQRVCP"
     gene            complement(4292..5341)
                     /locus_tag="VD788_14880"
     CDS             complement(4292..5341)
                     /locus_tag="VD788_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77595.1"
                     /translation="MFRTVSVSMLWVVVLGIIGSEPAHARYRYGQYTQGGSASGFFVF
                     GEGLITNPRNVDAVVATVETLENFGGGQNQLFQVNPTWGEDLAGRLGFGYGWATGNRV
                     SLTLWGFSADQSAQGSGPPGGATYFAIGPPIRTGSSYAGAQGSPGSYSIGTEVEAGTA
                     DVAFSREETLSESFTLEWSLGLRYASYEERSSGFYDEAALGSGTFGLNSFAADKRIES
                     EMFGVRAAFRGSYRFTQSFSVDGGLGFAFLDGEQVASSGLTPTGLTNSATTPSSFVSF
                     VDDSRSGATWNVDLTISWWVVDEAVRLWAGWEQQVWETLTVDLVRNLPGTVAPLTERD
                     SVAFSGYKIGVFVRF"
     gene            complement(5391..>7177)
                     /gene="lepA"
                     /locus_tag="VD788_14885"
     CDS             complement(5391..>7177)
                     /gene="lepA"
                     /locus_tag="VD788_14885"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXV77596.1"
                     /translation="QIRNFSIIAHIDHGKTTLSDRLLEKTGAVTDRERHDQMLDSMDL
                     ERERGITIKSHAVTLTYRAKDGRDYTLNLIDTPGHVDFSYEVSRSLAACEGALLVVDA
                     SQGVEAQTLANTYLAVEHDLEVIPVINKIDLPSADVERAREQIEQVVGIDTSATVAAS
                     AKEGLGIDEILEQVVALVPPPSGDAGAPPKALIFDSWYDPYRGVVMLVRVVDGSLEKR
                     MAIRLMNTGGSYEIEELGIFAPKPTPLERLGVGEVGFVIANIKKISDCQIGDTITAEH
                     NPTPRALPGFREVKPMVFAGLYPTDAAQFEDLRDAMDKLRLNDSAFSFEPESSGALGF
                     GFRCGFLGLLHMEIVQERLEREFDLSLITTAPGVSFVVTTTRGERFEVHNPSKLPERQ
                     FIDRIEEPVIEALIILPAEYLGTVFRLCEERRGVQKKMSYLSKTRVLLEYELPLSEVV
                     LDFYDKLKSGTRGYASLDYHFAGHRPSQMVKLDLLVNGEAVDALSLVCHRDKAEQKGR
                     ALAARMKELIPRQMFEVVIQAAVGGKILCRETIRPLGKNVLAKCYGGDITRKRKLLEK
                     QKEGKRRMKRVGRVDIPQDAFLAILKVD"
BASE COUNT         1143 a         2414 c         2405 g         1215 t
ORIGIN      
        1 ggaacccggc gaccgacgag acgcgcgcca tcacccgcga cttcgacccc gccgtcgtcg
       61 acgcgaagtg gagcgccggc gacggccgga tctacctgtt ggccgaagag cgcgacactc
      121 gtccgctgtt ccgtttcgac cccgagaccg acagcttcga gcggctcgac accggctacg
      181 acgtcaaccg cgagctggcg ctcgcgcgga acgcgccgac ggtcgtcctg ctggccagct
      241 cgccctgggt cgcggagcgg ctggtcgccg tcgacggcgc gcgcgacgcc gcacggacgc
      301 tcgagcaccc ggctggcgag tggctcgccg gagtgagtcg cgggacggtc gagccgtgga
      361 ccttcgtcgc ggcgaacggc aagacgatcg acggcagggt ctacgttccc cccgacttcg
      421 acgccggcaa ccggtacccg gcgatcgtct actactacgg cggtacctcg ccggtcggtc
      481 gcgagttcgg tggccgctac ccgaaggaat ggtgggcggc ccagggctac gtggtctacg
      541 tcccgcagcc gtcgggcgcg accggtttcg gccaggagtt ctccgccgcc cacgtcaacg
      601 actggggcaa gacgacgagc gaggagatca tcgagggcac gcggcgcttt ctcgaggccc
      661 atccgttcgt cgacgcaggg cgcgtcggct gcatcggcgc gtcctacggc gggttcatga
      721 cgatgctgct gtcgacgaag accgacctgt tcgcggcggc ggtgtcgcac gccggcatct
      781 cgtcgctcgc ctcgtactgg ggcgaaggct actggggctt cctctacagc tccgtcgcga
      841 ccgccgagtc gtacccgtgg aaccgcaagg acatctacgt cgaccagagc ccgctttacc
      901 gcgccgacga gaaccgagtg ccgatcctgc tgacccacgg cgtggccgac accaacgtgc
      961 cggtcggcga aagcgacgcg ttctacgtcg cgctcgagct gctcggcaag gaggtcgagt
     1021 acctgcagat cgaaggggag aaccactgga tcgtcgacca cgcgaagcgc gtcgtctggt
     1081 cgcggagcat cgtcgcctgg ttcgaccgct ggctcaagga cgagccgcag tggtggcagg
     1141 cgctctaccc gcccggcggg ccaccggcgc ccgacgagaa gtcgaaaacg gctcgctgac
     1201 cgccacggtc gacggtcgcg tgcgtgccgg cgtcgttcag ccggcggcgg gtcggacggt
     1261 cactgcgtgg cgttgaggaa tccgccggac tggttcatgt tcgtcgtcgt gcactcccca
     1321 tcctgtgtgg ggcagatgtg gaactcagtg tcatccgggc cggcgaagcc gaacgcgctg
     1381 ttctgcgccg tcgtcaacgc catgaagttg agcgtgatca gcgtctgggt gccgacgaaa
     1441 tcgacaccgg ggggtctttc gtcagtgatc gccgcaatga ccttgacctc gccgggcatt
     1501 ccctgatcca actctgcgtc aaaaaagagg tcgtctccgt tgctcgtcag gaacgaaccc
     1561 gcgctgctga agccgacgaa ctcggcgacc gccggatcgt aggtgacgtg gaacgcggcg
     1621 ctcaagaggt cgttccggcc cgtgatctgc accacgacgc tgaagctggc tccgttcgag
     1681 ccacccggcg cgaggctgat cgtgttggcg cccggcgccg ggttgccggc gacgaaggtc
     1741 gcgaccaggt tgcccatccc gccgccgccc ccgccgctgc tgccgccgcc gccgcaggcc
     1801 gaggccaagg taccggtcag cagcaccgca ccgaggcaca aggttcgata gagcagtggt
     1861 tgcctgttca tggggggcct catcacggac aaacccgctg gaagtcggcg gcgatcaccg
     1921 ccaggtcgac gccgtcgacc atcccgttct ggtcgagatc gacggccgcg ttgtaacgga
     1981 cgtggacgcc ggtttgcgcg ccgaacgcga cggccaggcg acccacgtcg atcccgtcga
     2041 cctcgccgtc gccgctgacg tcggccaacg accgctgggc cgcggccgtc ccaccggtga
     2101 agcccatgtt ggcgcgaccg ccgttcggcg cgggctcggg cgcgaacggg agcgcgggat
     2161 tcgccgcgtc gatcgtcggc gagcaaagcc cggggaggaa cgacgcgtcg agaagcgggt
     2221 cggtcgtgat gttgcccgtg ccgctgacga cccagccgct cagattgccc tgcgggtggc
     2281 cgaacagatc gttgtaggtc gtgacgaccg agaactcggc ggtcccgccg acggtcttga
     2341 ctccggcggt tccgggcgac gggccgccga aaccgacgcc gttgttgccg gacacgatac
     2401 tgctgtcgag cgtcagcgcg gcctggccgt cggtggcgct cagggggtcg gccgaggtca
     2461 gtgattcgag gtggaacccg cccggttcgg cctggttgtc gagcgtgttc gaggagatcg
     2521 tgttccacgt cgcgtcgacg tcggccacgg cgtcctggcc gcgcgcggtc agcaaggcga
     2581 acacaccacc gccgacggcg ttgcgcacgt tgctgtcggt gctgttgccg gtcacgcggt
     2641 tgttgcggaa gtcgaggaac gcgtcggccg gggcgaccga gaccgctccc ggaggcagcg
     2701 cgtcggcctc gacgaacatc gatacgccgc cgccgagcag cttggcgctg ttgccggtga
     2761 tcgtgttgtt gcggacactg aactccatgt tcggcgaatc cgagctcaac gactccatcg
     2821 tgagcagcag gccgccgccg ccgaacgact cgtcgaggtc gtcgatcgtc gtcgtgccga
     2881 ccacgccggc gacgttgttg gtcagcgtgt tgttctcggc gatcatcgag aacgcctgcg
     2941 ggttgttcat gtcgagcacc gagacgaaca gctccatccc accgccgcct cggccggcgc
     3001 ggttgccgct gatttgattg tcgctgacgt gatactggtg gtcgatcgac gaggcgatca
     3061 ccgtcgggaa gacggtgagc tgggctccgc cgccgaagtc gagcgtcgag ttgccggtga
     3121 tcgtgttgtc ctcgacctcg atccgctcgg tgccgtagcc gtaggtcaag ccgtacagcc
     3181 cggcgccgta accgatcgtc ccgctctggc cgggggtcga cgaggtgttg tcgctgatcg
     3241 tgttcgaggt gatcgtgacc gtggcctggc gctgatcgtt ggtgaagaca gtggcgtaga
     3301 tcccgccgcc gttcgactcc gacgtgttcc cgtcgatggt gttgttgttg atcgtgatcg
     3361 tcgcgtcgcc gtcgaacggg caggtgatcg ggtcggggtt ctgcgcgttg accgacagac
     3421 cggcggcgat cgacatgccg ccgccctgca gcgtcaccga gttgcccgtg acctggttgt
     3481 ccgagatcgt catgaccgtg tcgccgtagt agcaattgcc cgagtagacg tagattccgc
     3541 cgccgatcgt cgacgtgttg ccggtgacga cgttgtcggt gatctcgacc gagccgacgc
     3601 cgaagattcc ggaggccgtg ccgccggtga cggtgaaccc ggtgaagcgc gaagccgtgc
     3661 cgccgatgtt gacgaccgac gccggcgcca gcgaggtggc gaactcgcag tcgaccgacg
     3721 gcacggtcga gccgtcgatg atcgtcacgc tgttgtccgt gctggtcagc cacgcgttgg
     3781 cgacgacctc gatgctcttc tgcgtatcga ggtcgaacag gaacaggcac tcggtgtagg
     3841 tgccgggcag gacgcgaatc tcggcgccgt ccgccgcgaa gtcgtaggcc gcctggatcg
     3901 tgcagaacgg cgccgccggc aaaccggacc cggcgtcggt acaggaaccg ttctgggtgt
     3961 cgacgaagat ctgggcttgg gcaggaatca ccgtcgcggc caccgcgacg atccacgtgc
     4021 cgaggatccg tttgctgttc acctcagctc ctcctcatcg atacagttcc gtcctgccga
     4081 cgcgtaggcc gccaccgccg gacaggactc ggaagtgctg taacggggtc ggggggtgga
     4141 acacagtgct cgtcgttgat ctcgcttctc gactcgatcg gaatccgtgc tggcttttgg
     4201 gtatacccag cgatcggggg gttgtcaagc gcacgccgtc gctcgcggac gacggatccg
     4261 cagaaaacct ccaaaaagcg caagccaccg ctcaaaaccg gacgaagacc ccgatcttgt
     4321 agccggaaaa cgcgacactg tcgcgctcgg tcagcggcgc gacggtcccc gggaggttcc
     4381 gcaccagatc gaccgtcagc gtctcccata cctgctgctc ccagccggcc cacagccgga
     4441 ccgcctcgtc gacgacccac caggagatcg tcaagtcgac gttccaggtc gcgccggacc
     4501 ggctgtcgtc gacgaacgag acgaacgacg aaggcgtcgt cgcgctgttg gtcagtccgg
     4561 tcggcgtcag acccgaggat gcgacctgct cgccgtcgag gaaggcgaag ccgaggccgc
     4621 cgtcgaccga aaacgactgc gtgaaccggt agctgccgcg gaacgcggcg cggacgccga
     4681 acatctcgct ctcgatccgt ttgtcggcgg cgaagctgtt gagcccgaag gtgccgctgc
     4741 cgagcgcggc ctcgtcgtag aagcccgagg accgctcctc gtagctcgcg taacgcaacc
     4801 ccagcgacca ctcgagcgtg aacgactcgg acagcgtctc ctcccgcgaa aaggccacgt
     4861 cggccgtccc ggcctcgacc tcggtgccga tcgaatacga ccccgggctg ccctgggcgc
     4921 cggcgtagct gctgccggtg cggatcggcg ggccgatcgc gaaataggtc gcgccacccg
     4981 gcgggccgct gccctgcgcc gactggtcgg cactgaagcc ccacagcgtc agcgagaccc
     5041 ggttcccggt ggcccagccg tagccgaaac cgagccgccc ggccagatcc tcgccccagg
     5101 tcgggttgac ctgaaacagc tggttctgcc cgccgccgaa gttctcgagc gtttccacgg
     5161 tggcgaccac cgcatcgacg ttgcgcgggt tcgtgatcag cccttccccg aagacgaaga
     5221 agccgctcgc actgccgccc tgggtgtact gtccgtagcg gtacctggcg tgcgcgggtt
     5281 cggatccgat gatccccagc accacgaccc acaacatcga aaccgagact gtccgaaaca
     5341 tgttcactcc cggtagctct ggcccggcct agattaaccc aaatccgagt tcagtcgacc
     5401 ttcagaatcg ccagaaacgc gtcctgcggt atgtcgaccc gcccgacgcg cttcatcctg
     5461 cgcttgcctt ccttctgctt ctcgagcagc ttgcgcttgc gagtgatgtc accgccgtag
     5521 cacttcgcca gcacgttctt accgagtggg cggatcgtct cgcggcagag gatcttgccg
     5581 ccgaccgccg cctggatcac cacctcgaac atctgccgcg ggatcagctc tttcattcgc
     5641 gccgccagcg cgcgcccctt ctgctcggcc ttgtcgcgat ggcagacgag cgacagggcg
     5701 tcgaccgctt cgccgttgac cagcaggtcg agcttgacca tctggctcgg gcggtgtccg
     5761 gcgaagtggt agtcgagcga ggcgtagccg cgcgtccccg acttgagctt gtcgtagaag
     5821 tcgagcacca cctccgacag cggcagctcg tactccagca gcacgcgcgt cttcgacagg
     5881 taggacatct tcttttgtac gccccggcgc tcctcgcaca ggcggaagac cgtgccgagg
     5941 tactcggccg gcaggatgat cagcgcctcg atcaccggct cctcgatccg gtcgatgaac
     6001 tgccgttcgg gcagtttgct cgggttgtgc acctcgaacc gctcgccccg ggtcgtcgtc
     6061 accacgaacg acactcccgg cgcggtcgtg atcaggctca ggtcgaactc gcgctcgaga
     6121 cgctcctgca cgatctccat gtgcagcagg ccgaggaaac cgcagcggaa gccgaagccg
     6181 agcgcgcccg agctctccgg ctcgaacgag aacgcggagt cgttgagccg cagcttgtcc
     6241 atcgcgtcgc gcaggtcctc gaactgcgcg gcgtcggtcg gatacagccc ggcgaagacc
     6301 atcggcttga cctcgcggaa gccgggcagg gcgcgtggcg tcgggttgtg ctccgcggtg
     6361 atcgtgtcgc cgatctgaca atcgctgatc ttcttgatgt tggcgatgac gaagccgacc
     6421 tcgccgacgc cgagccgctc gagcggcgtc ggcttcggcg cgaagatccc cagctcctcg
     6481 atctcgtagc tgccgccggt gttcatcaag cggatcgcca tccgcttttc gagcgacccg
     6541 tcgaccaccc gcacgagcat cactacaccg cggtaggggt cgtaccacga gtcgaagatc
     6601 aacgccttgg gcggcgcccc ggcgtcgccc gacggcggcg ggacgagcgc gacgacctgc
     6661 tccaggatct cgtcgatgcc gagcccttcc ttggccgagg cggcgacggt cgccgaggtg
     6721 tcgatgccga cgacctgctc gatctgctcg cgcgcccgct cgacgtcggc gctcggcagg
     6781 tcgatcttgt tgatcaccgg gatcacctcg aggtcgtgct cgaccgcgag ataggtgttg
     6841 gccagcgtct gcgcctcgac cccctggctc gcgtcgacga cgagcagcgc gccctcgcac
     6901 gccgccaggc tgcgcgagac ctcgtaggag aaatcgacgt gacccggtgt atcgatcagg
     6961 ttgagcgtgt aatcgcgacc gtccttcgcc cggtaggtga gcgtcaccgc gtgagacttg
     7021 atcgtgatgc cgcgctcgcg ctcgagatcc atcgagtcga gcatctggtc gtgtcgctcg
     7081 cgatcggtga cggcgccggt cttctcgagc agccggtcgg acagcgtggt cttcccgtga
     7141 tcgatgtggg caatgatgct gaagttgcgg atctgtc
//
LOCUS       DASPGN010000514        18909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_681130, whole genome shotgun sequence.
ACCESSION   DASPGN010000514 DASPGN010000000
VERSION     DASPGN010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 18909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18909
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681130"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="VD788_14890"
     CDS             complement(<1..115)
                     /locus_tag="VD788_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXV77597.1"
                     /translation="MKRTILVTGGAGFLGSHLCDRLLAEGFAVIAMDNLLTG"
     gene            complement(137..1228)
                     /locus_tag="VD788_14895"
     CDS             complement(137..1228)
                     /locus_tag="VD788_14895"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXV77598.1"
                     /translation="MSDTRLAELVLAVVRRNPGDVRRLAARGEVRPAALARACRESDV
                     HPWVHHLLTHDSPRSVGDAAVAALAASRRKVRDDTMLLLAHAERALDALLAAGVVPIA
                     LKGLDFLHRLYPRVDLRATDDVDLLIRRRDLKLALDALRGVGWLLPAHDKATHYVRSS
                     HHLPLHSPGALRVDLELHWNLAQERRYTIDPDGLFDRALPLDVAGRSILRLGDEDIVA
                     HLLIHHFSHYFDRRLKWLVDLQRVEEARPIDWPAVARRLREWSGTAAAGISLMHLRKL
                     DPALIPPHAVAELDVAGWRRVLTWPLRSSHPLELFRGTRRRRTQLYLAAVMIEHPSQL
                     PGWLRHRARRGDDPADNPLDDHSRSAMTG"
     gene            1332..2054
                     /locus_tag="VD788_14900"
     CDS             1332..2054
                     /locus_tag="VD788_14900"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV77599.1"
                     /translation="MNRHPKRLVEAVAPSIAYWYLRLLQLTTRRSYRNQQALAQARGI
                     GGPYIMAFWHSRLLLMRFGYPDDNAVVLQSHHRDSQMLGRVMSRFGTGQVWGSTTRGG
                     MTAVREVLRRIRLGHDVAIAPDGPRGPRRRAQSGVITIARLSGKAIVPMAYSARWARR
                     LSSWDRLLVPLPFSRALYLYGEPLVVGRRVDDGEEERLRQRLEDVLNRLTDEVDREMG
                     IPRTEPAPLDDDLGRVRPPERG"
     gene            2051..3214
                     /gene="lpxB"
                     /locus_tag="VD788_14905"
     CDS             2051..3214
                     /gene="lpxB"
                     /locus_tag="VD788_14905"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HXV77600.1"
                     /translation="MSRAGRVMLSAGEASGDRLGAGLARALLERRPQLELFGMGGEQM
                     AEAGVEVIQDASEVAVVGFSEVLAHLPALYAAKRRLESALAERAPDLLVPIDFPDFNF
                     LLSARARRLDVPVVYFVSPQIWAWRRGRVRRIRRFVRRMLVLFPFEVDFYREAGVPVS
                     FVGHPVAERPADDDATDEWRAGVGFDPALRLVALLPGSRRSEVDRLLPVMLAAAKRLA
                     TARDGLGFVIPLARGLDRRAVEARVAESGLRETRLVERHFPQVLRSCAAGVVASGTAS
                     LEAAVLGLPIVVVYRVGRFSYWIGRALVRVDHIALPNLVAQRRVVPELVQDDCTPQAI
                     ADAVASFLDDPGRVAQLRGELAEVERRLGGPGVFERAADAVLAELDAATGRTA"
     gene            3380..4255
                     /locus_tag="VD788_14910"
     CDS             3380..4255
                     /locus_tag="VD788_14910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXV77601.1"
                     /translation="MTGFGQGAAESGGLRVSVELRGVNNRFNDLRFRMPAELAHLEPE
                     LRRRAMRRVRRGRVEIGIRVEPPGGSQPRPILNQPLLDEVTAVSKRLETELGLTGPLR
                     PGEVLSIPGMFKQESLELGWSGSNDQLVFDAFDGALARFDADCLREGEALRADIVARV
                     ASMAGHSAAARRLAPTVPETLRAKLLHRLDSLRGAIELDAARVAQEAAFLADRADVTE
                     ELVRFDGHLTQTRALLDEPDGEPLGKRIEFLFQEMHREANTVCSKSADLELTRHALAI
                     KLEIEKAREQAQNLE"
     gene            4258..4899
                     /gene="gmk"
                     /locus_tag="VD788_14915"
     CDS             4258..4899
                     /gene="gmk"
                     /locus_tag="VD788_14915"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXV77602.1"
                     /translation="MSERRQGILFVIAAPSGTGKSSVAREVLARDAELEFSISYTTRA
                     PRDGERDGREYHFIDRDEFARMRERQAFLESAEVFDQLYGTGLEATRRSLATGKDLLL
                     DIDVQGARQVTGGPLEAVTIMIFPPDYRTLTERLERRGSEDRAQSRRRLAKARAEAED
                     YLSFDYLVVNRDLEAAVRDVSAIVHGERRRTRRCATEARRVLATFPVEGSTTE"
     gene            4947..5171
                     /gene="rpoZ"
                     /locus_tag="VD788_14920"
     CDS             4947..5171
                     /gene="rpoZ"
                     /locus_tag="VD788_14920"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXV77603.1"
                     /translation="MFKLPEDIDNKYRFVTLASKRAEQLQFGALPRVADTARKSTVIA
                     QEEVASGLVELWAPEREGAEGAVADEEEEE"
     gene            5184..6377
                     /gene="coaBC"
                     /locus_tag="VD788_14925"
     CDS             5184..6377
                     /gene="coaBC"
                     /locus_tag="VD788_14925"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HXV77604.1"
                     /translation="MLIALGVSGGIAAYKTPEIVRALDRAGAEVQVLLTQNACEFVTP
                     LTLQTLSRNRVLIGQYDLDEEQTIRHIELTRRVSALVVAPATANFLAKMARGVADDLL
                     STFYTAVTAPVVVAPAMNTRMWLHPATQANLAVLRGRGVTVIEPESGWLAEGETGQGR
                     MAEPGEIARAAWHAGLRSSQLAGKKIVVTAGPTREPIDPVRFVSNRSSGRMGHALAAA
                     AARRGARVVLVSGPVDLPPPYGVERVVVETSAEMRRAVLDAREGAEAVFMAAAVSDFI
                     PRRSPSKIKKREATLTIELERGPDILSELGQSRCERVLVGFAAETESLVEHAREKLER
                     KRVDFIVANDVSRRDIGMEADDNAVTILSRDGGIVEVPKASKAEIADVILDRTLGAVV
                     VEEPS"
     gene            6374..7180
                     /locus_tag="VD788_14930"
     CDS             6374..7180
                     /locus_tag="VD788_14930"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXV77605.1"
                     /translation="MNPTSGAGGRSATRELVEWVDYLREIGVRELCVGETGSPAAAPG
                     ALPAREEGTPPPTRPAAPPAPTRPVLAALAVFSGPGYPDPGARLQQIRDELGECSRCK
                     LHATRNRIVFGVGDPRARLMFVGEGPGADEDARGEPFVGRAGKKLDEMISAIGLTRAE
                     VYIANVVKCRPPKNRDPESDEIATCSPFLELQIEAIRPRVIVTLGAPAARTLLNTRVG
                     ITRLRGNWHSFRGIPVMPTYHPAYLLRAYTRENRLKVFEDLKAARARMDA"
     gene            7186..9384
                     /gene="priA"
                     /locus_tag="VD788_14935"
     CDS             7186..9384
                     /gene="priA"
                     /locus_tag="VD788_14935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXV77606.1"
                     /translation="MAQPRYAAVAVPVPVRRLFSYSIDEALAPRITTGVRVRVPFGRR
                     TLVATIVEFPAARPAADVEPKPVLEIVDDAMLDPRLLELTRFVADYYLCSWGEAIETA
                     LPPGCGTAGPRVVRLGDGASLEAIPRRATARRRLFRRVAERDGPLSLASLGTNERRLL
                     PTMVEDGLLVVVRGQTGSDREPPEPRHAPPAEAPTPTAAQARVLERIDPAIERRGFRP
                     FVLFGATGSGKTEVYFRAAERALAGGRGVLYLVPEIGLTPLLLGAVRRRFRGEVTVLH
                     SGLGRRERRDGWNLVRTGRSRFVIGTRSAVFAPLADIGLIVVDEEQDGSYKQSETPRY
                     NGRDLAVVRAHAHEATIVLGSATPSMETFRHARAERYELLRLGDRIERRPLADVRIVD
                     MREVYRARGEVTPLSPLLAGELRDCLERGDQALILRNRRGWAAALHCPSCGDRVECPN
                     CTIALTWHRAARRLRCHYCGLEQGYPDACPRCGAAELNPLGEGTERIEDRIKRLLPAA
                     RIDRMDRDTIRRRGAHEKMLRRFERGEIDVLVGTQMIAKGHDFPRVTLVGVLSADQAL
                     GIPDFRAAERAFQLLTQVAGRAGRGASPGRVVVQAFDADHPVLGLAARQDYEAFYDRE
                     IRYRRALRYPPLSALVQIIVQDKDPITARGWSERLAEALREQDPERLLLSGPGPAPIE
                     RLRGRVREQILVRGAGRRRLVETVDRALRAVEGQIPRRAITVDVDPLSLL"
     gene            9391..10875
                     /locus_tag="VD788_14940"
     CDS             9391..10875
                     /locus_tag="VD788_14940"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HXV77607.1"
                     /translation="MSPNTVPQGPLVVGSLLASDVATAGAMLAGAPPGALLLELRADR
                     LRADEVAAIVRRTERPLIVTVRRSADGGGFDGSEHERRALLTAALDAGARFVDVEWDR
                     PLAALADDPSLTERVILSHHGVACRVRALERVGRAMRARDAAAYKIVASAAEVDELPA
                     IRALLARRPLAGRPLAAFADGRAGVLSRLLAPSWGSWATYAALDRPTAEGQFGVGEML
                     DGYDVLAISDRTRRYALVGSTVSNSPSPAMHNAALRQLGLDARYFAVTTDRFGAFVRL
                     ARSPAAALAGFGVTMPFKEIAARRSRPADSIARASGAVNTVVVGPGGWVGYNTDGPAA
                     LASIGERIDPAGCRVAVLGAGGTGRAIACSLAAAGARVRLFNRSAERARRAARALGVE
                     WAPEERLDEWRWDVLVHATPRGAGGERFIAPERLRGAVVLDAVYRPRETMLCADARRA
                     GLQVIDGRRLLLEQALLQFRLLTTHDARVETLRAAAVAWLGSGW"
     gene            10977..11603
                     /locus_tag="VD788_14945"
     CDS             10977..11603
                     /locus_tag="VD788_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77608.1"
                     /translation="MRVIVCTASAVLLLVFAGCAARQPIEGQPTTALDRKLSTFAFIE
                     EGDLITLIVGTQAARYREKSEFMPVEVMIANNGLKNLVLTRESFTLIDEQGNRYPLAR
                     PQELYEGYDFLDLDRQALSTLPDVTTNKFAAYTRYGSRFSPTRSTVADLTGSGSLVRD
                     AISVPKFGYIWDFLYFPRPVTGIKGHRFELFVESAGLEDPVFVKFEVK"
     gene            11606..12205
                     /locus_tag="VD788_14950"
     CDS             11606..12205
                     /locus_tag="VD788_14950"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HXV77609.1"
                     /translation="MARLLLLDNYDSFSWNLVQYLAELGADVDVKLHDEVDVSWCVSQ
                     RFDAIVISPGPGRPEGAGISMDLIREVGGSVPLLGVCLGHQAIARVYGAEIIGAPTLM
                     HGKTSPIVHDGRGIFAGLTPSFEATRYHSLVVDESTLDPTRFLITARTPEGVIMAIEH
                     RRFPVFGVQFHPESVLTLEGKRLLGNFLGLAAANREQAV"
     gene            12224..12742
                     /locus_tag="VD788_14955"
     CDS             12224..12742
                     /locus_tag="VD788_14955"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF009763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000334 - 3-hydroxyanthranilate
                     3,4-dioxygenase activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HXV77610.1"
                     /translation="MQQLSPYNLKQWIEENRHLLKPPVGNKIIWQDGEFLVMAVGGPN
                     RRKDFHVEDGPEFFYQIEGDITLRIIEDGEPRDIQIREGEIFLLPPRIPHSPQRPAGT
                     VGLVIERVRKAGERDHLVWFCESCGDTLHDAAFELTDLGTQLKPIIERFYGDQKLRTC
                     GSCQTTMQVPKV"
     gene            12739..13758
                     /locus_tag="VD788_14960"
     CDS             12739..13758
                     /locus_tag="VD788_14960"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV77611.1"
                     /translation="MRPLKIDVHTHVLPRSWPDLAERYGYPGFVRLEHDGPDRARMMR
                     EGECFRVIESNCWSAERRLEECDRTGVDVQVLSTVPVMFSYWARPEHALDLSALLNDH
                     IAELVSRHPRRFAGLATVPLQDPERAIAELERAVGQLGMCGVQIGSHVNDWELDQPEL
                     LPFFRRAAELGAAVFVHPWEMLAAERMSRYWLRWLVGMPAETTLAICSMIFGGVLERL
                     PRLRVGFAHGGGSFPATVGRIQHGFEVRPDLCAVRNDVSPADYLRRIYYDSLVHRADA
                     LHYLIRTVGVDRIFLGSDYPFPLGERLPGALIERLEGIDETDRVRLLSGTALEFLGRE
                     VADFG"
     gene            13769..14515
                     /gene="kynU"
                     /locus_tag="VD788_14965"
     CDS             13769..14515
                     /gene="kynU"
                     /locus_tag="VD788_14965"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HXV77612.1"
                     /translation="MSPRIFHPGEELARELDARDRLAAFRDRFHLPPDDDGSPLAYLC
                     GNSLGLQPRATRASLERELEDWARLAVDAHFDGRTPWYSYHETLRGPLARLVGALPGE
                     VVAMNSLTVNLHLLMVSFYRPTPERHRILIEQHAFPSDNYAVRSQARFHGFDPGQAVI
                     VARARDGETTVRSEDLESLIEREGPTIALILIGGVQFLTGQLFDLARIAECGRRQGAT
                     VAFDLAHAVGNVPLALHDWGVDFAVWCSYK"
     gene            14537..15052
                     /locus_tag="VD788_14970"
     CDS             14537..15052
                     /locus_tag="VD788_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77613.1"
                     /translation="MAGCFVHRRHHGKQLPRLAGWWGNDPATRFRMHLEPEFVPVSSA
                     DAWQLSNPPILAAAPLRDSLALFDEAGMAALRDKSLALTAYLHRLVAEVADRRLRLLT
                     PDDPAARGCQTSIEVARDGRALFGAMRRCGVVGDFREPGVIRVAPVPLYNSFHDVWRF
                     GRALAEWSTAR"
     gene            15049..16398
                     /locus_tag="VD788_14975"
     CDS             15049..16398
                     /locus_tag="VD788_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXV77614.1"
                     /translation="MSGRGATGRKETVLVAGAGLGGALIAIYLARAGHRVRLFERRSD
                     PRKLTGDRGRSINLAISTRGLAALRGVGVDGPVVEMAVPMRGRMMHGVEGRLTFQPYG
                     TTGQAIHSVSRLGLNIALIDEAERHPEVVIEFDSRLVDVDPDGPELLLESPDGSTRRV
                     AGDRLIGADGAYSAVRARLQRLDRFDYSQSYLEHGYKELTVPPADDGGFRLEPEALHI
                     WPRGGFMMIALPNVDRSFTCTLFWPLEGATSFRQIDDAPGLRAFFGRWFPDAVPLMPR
                     LAEDYFANPTSSLVTVRCHPWHHLDRVVLLGDACHAIVPFYGQGANAAFEDCVVLDRA
                     IHQHAPDWGAAFAAYGRQRKPHVDVLADLALENFIEMRDRVASRSFLWQKRLEKGLHR
                     AFPERFVPLYSMVTFSQLPYGDAVERARRQWRLVRRIAATAAAAALIAAGLVLGLIW"
     gene            16412..17206
                     /locus_tag="VD788_14980"
     CDS             16412..17206
                     /locus_tag="VD788_14980"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HXV77615.1"
                     /translation="MSDPTMNYSSYLELDRLLSLQRPRSRPVEHDELLFIVIHQVYEL
                     WFKQLLHEIDKVKLDFSAGELFAAIATFKRMRTVMKTLVGQLDILETMTPLSFSAFRS
                     RLETASGFQSLQYREVEFALGYKRQSVMRYIDSSWSGIERVERRLGEPSLVDHFYDFL
                     ERRAVTIPEAVKRRRGTPQPTLPDETVQRGLLELYHGSPEAAILFELMIDFDEGLQEW
                     RYRHVKIVERTIGSKQGTGGSPGVEFLKKSLFLSVFPDLWAIRHEF"
     gene            17208..17828
                     /locus_tag="VD788_14985"
     CDS             17208..17828
                     /locus_tag="VD788_14985"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HXV77616.1"
                     /translation="MKLYDISPTVSPSLAVWPGDTPPRREVLLDIDRGDNLTLSTLHA
                     TVHLGAHADAPSHYGAGAPAIDRRPLDAYLGRCQVVRVDVRSGSRLGAAAVPGSPAAR
                     LLFATGSYPDPDEFRRDFAALSVELIDTAHRRGVRLIGIDTPSVDLFESKELPAHHAC
                     LARDVAILEGLVLDDVPVGVYELIALPLKLAGFDASPVRAVLRSID"
     gene            complement(17854..>18909)
                     /locus_tag="VD788_14990"
     CDS             complement(17854..>18909)
                     /locus_tag="VD788_14990"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HXV77617.1"
                     /translation="QPSVDEYLSKGYSYSRSGNPTVRALERKLTALEGGADCSCFSTG
                     MAAIQAVLLATLNAGDHAVVSDVAYGGTYRLCTKVLARFGVEFTFADTASPAAVRDSV
                     RDNTRLILTETPANPTLKLTDIAAISEIARERNVLHAVDNTFLTPYYQRPLELGADLV
                     VHSTTKYIDGHNATVGGAVVARTAELDGQLRFIQNAAGTIVSPQVAFLTLQGCKTLSV
                     RMDRQSANAMAVAQYLERQPKVDRVCYPGLESFPQHALARKQASGFGAMVWFEVRGGV
                     AAGKKLMDAVELWTLAENLGSIESLVTHPATMTHADVEAQERKRVGITDGLVRLSVGL
                     EDAEDLIAALADALDRL"
BASE COUNT         2721 a         6470 c         6737 g         2981 t
ORIGIN      
        1 taccggtcag caggttgtcc atcgcgatca cggcgaagcc ctcggcgagc agccggtcgc
       61 atagatggga gccgaggaag ccggcaccgc ccgtcacgag aatcgtccgc ttcaccctcg
      121 ccccctcagc cggccctcag ccggtcatcg ccgatcgact gtggtcgtcc aacggattat
      181 ccgccggatc gtcgccgcgc cgcgcgcgat gtcgcaacca gcccggcagc tgcgacgggt
      241 gttcgatcat caccgcggcg agatagagct gcgtgcgacg ccggcgggtg ccgcggaaca
      301 gctcgagcgg atgcgacgat cgcagcggcc aggtcaacac acgccgccag ccggcgacgt
      361 cgagctcggc gacggcgtgt ggcgggatca gggccgggtc cagtttgcgc aagtgcatca
      421 gcgagatccc cgccgccgcc gtgccgctcc actcgcgcag ccgtcgcgcg accgccggcc
      481 aatcgatcgg gcgcgcttcc tcgacccgtt gcaggtcgac cagccacttg agccgtcggt
      541 cgaagtagtg cgaaaaatgg tggatcagca ggtgggcgac gatgtcctcg tcccccagcc
      601 gcaggatcga ccggccggcg acgtcgaggg gcagcgcgcg atcgaacagc ccgtcggggt
      661 cgatcgtgta gcgccgttcc tgcgccaggt tccagtgcag ctcgaggtcg acgcgcagcg
      721 cacccgggct gtgcagcggc agatggtggc tgctgcggac gtaatgggtc gccttgtcgt
      781 gcgcgggcag caaccaaccg acgccgcgca gagcgtcgag cgcgagcttg agatcgcggc
      841 ggcggatcaa cagatcgacg tcgtcggtgg cccgcaagtc gaccctggga tagagccgat
      901 ggaggaaatc gagtcccttg agcgcgatcg gaaccacccc ggcggcgagc agcgcatcga
      961 gcgcgcgctc ggcatgcgcc aacaacagca tcgtgtcgtc gcggaccttg cgacgtgacg
     1021 cggccaacgc ggcgaccgct gcgtcgccga ccgatcgcgg gctgtcgtgc gtcaacaggt
     1081 ggtggaccca cgggtggacg tcgctttcgc gacaagcccg ggcgagggca gcgggtcgaa
     1141 cctcgccgcg cgccgcgaga cggcgcacgt cgcccgggtt gcgccggacg acggcgagga
     1201 ccagctcggc caatcgagtg tcagacatct ccacatccac ggggtcaggc aaagatagcg
     1261 gcggccggcg ggggcgtcaa cgcgacgttg acggtcatcg ccggagcgat tagactttgc
     1321 cgccccccaa tatgaaccgc catcccaagc gcctcgtcga agccgtcgcg ccgtcgatcg
     1381 cctactggta cctgagattg cttcagctca ccacgcggcg gagctaccgc aaccagcagg
     1441 cactggcgca ggcgcgcggc atcggcggac cgtacatcat ggcgttctgg cactcgcgcc
     1501 tgttgctgat gcgcttcggc taccccgacg acaacgccgt ggtgctccag agccatcacc
     1561 gcgattcgca gatgctcggc cgcgtgatga gccgcttcgg caccggtcag gtctggggct
     1621 cgacgacccg cggcggcatg accgcggtgc gcgaggtgct gcgtcggatc cgtctcggcc
     1681 acgacgtggc gatcgcgccc gacggtccgc gcgggccgcg acgccgggcg cagagcggcg
     1741 tgatcacgat cgcccggctg tcgggtaagg cgatcgtgcc gatggcctat tccgcccgct
     1801 gggcacgccg cttgtcttcc tgggaccgtc tgctcgtgcc gctgccgttc tcccgcgcgc
     1861 tgtacctcta cggagagccg ttggtcgtcg gccggcgtgt cgacgacggc gaggaggagc
     1921 ggctgcgcca gcgactcgag gacgtgctga accgcctgac cgacgaggtc gatcgcgaga
     1981 tggggatccc gcggaccgag ccggcccccc tcgacgacga tctcggccgg gtgcgaccac
     2041 cggaacgcgg atgagtcgcg caggacgcgt gatgctgagc gccggcgagg cctcgggcga
     2101 ccggctcggc gccggcctgg cccgcgcgct gctcgagcgc cgaccgcagc tcgagctgtt
     2161 cggcatgggc ggcgagcaga tggccgaggc cggcgtcgag gtgatccaag acgcgagcga
     2221 ggtcgcggtg gtgggcttct cggaggtgct ggcccaccta ccggcgctct acgcggcgaa
     2281 gcgccggctg gaatcggctc tcgccgagcg tgcccccgac ctgctggtgc cgatcgactt
     2341 tcccgatttc aacttcctgc tctcggcccg cgcgcgccgt ctcgacgtgc cggtcgtcta
     2401 cttcgtcagc ccgcagatct gggcctggcg gcgcggccgg gtccgcagga tccgccggtt
     2461 cgtccggcgg atgctcgtgc tgttcccgtt cgaggtcgat ttctaccgcg aggcgggggt
     2521 tccggtcagc ttcgtcggcc acccggtggc cgagcggccg gcggacgacg acgcgacgga
     2581 cgaatggagg gccggggtcg gcttcgatcc ggcgctccgt ctggtcgcgc tgctcccggg
     2641 cagccgacgc tcggaggtcg accggctgct gccggtgatg ttggccgccg cgaaacggct
     2701 ggcgaccgcg cgggacgggt tgggcttcgt gatcccgctt gcccgcggcc tcgatcgccg
     2761 cgccgtcgag gcgcgggtgg cggagtccgg gttgcgggag acgcggctgg tcgaacggca
     2821 cttcccgcag gtcctgcgct cctgcgccgc gggtgtcgtc gcctcgggca ccgcgagcct
     2881 cgaggcggcc gtcctcgggc tgccgatcgt cgtggtctat cgcgtcggcc ggttctccta
     2941 ttggatcggc cgagcgctcg tgcgcgtcga tcacatcgcg ttgcccaatc tcgtcgccca
     3001 gcgccgggtg gtgccggagc tggtccagga cgactgcacc ccgcaagcga tcgccgacgc
     3061 cgtcgcgtcc ttcctcgacg atccgggccg ggtcgcgcag cttcggggcg agctggccga
     3121 ggtcgagcgc cgcctgggtg gtcccggggt gttcgagcgg gcggcggatg cggtgctggc
     3181 ggaactcgac gcggcgacgg gtcggacggc gtgatcccgc ggggacgccg ggagcttcgg
     3241 ccgttcgttc ccgctgccgg tgtcgagtgt ctttgacgat cagacggatc ggcggtccga
     3301 tcgccgccgg ggaggccgcc ggcggcggcc ggccggccag cggtggtatg atctgcggtt
     3361 tgtgatgcga gtccgctcga tgacagggtt tggccagggc gcggccgagt ccggcggcct
     3421 gcgggtttcg gtcgagttgc gcggcgtgaa caaccggttc aatgacctgc ggttccgcat
     3481 gccggccgag ctcgcgcatc tcgagcccga gctgcgacgc cgggcgatga gacgcgtccg
     3541 tcgagggcgg gtcgagatcg gcatccgcgt cgagccaccc ggcggctcgc agccgcgtcc
     3601 gatcctgaac cagccgctgc tcgacgaggt caccgcggtg tcgaaacggc tcgaaaccga
     3661 gctcgggctg accggcccgt tgcggccggg cgaggtcttg tcgattccgg ggatgttcaa
     3721 gcaagagtcg ctcgagctcg gctggagcgg gtcgaacgat caactggtgt tcgacgcctt
     3781 cgacggggcg ctcgcccggt tcgacgccga ctgcctgcgc gagggcgagg cactgcgcgc
     3841 ggacatcgtc gcgcgcgtcg cgtcgatggc cggccactcc gcggcggccc gccggctggc
     3901 gcccaccgtc cccgagacgt tgcgggccaa gctgcttcac cggctcgact cgctgcgcgg
     3961 cgcgatcgag ctcgacgcgg cccgcgtggc acaggaggcg gcgttcctcg ccgaccgtgc
     4021 cgacgtgacc gaagagctcg tgcgcttcga cggacatctg acacagaccc gcgccttgct
     4081 cgacgagccg gacggagagc cgctgggcaa gcgcatcgag ttcctgtttc aggagatgca
     4141 ccgcgaggcg aacacggtct gcagcaaatc ggccgatctc gagctgacgc gacacgcgct
     4201 ggcaatcaag ctcgagatcg aaaaggcacg cgaacaggcc cagaacctgg agtgacgttg
     4261 agcgaacgac ggcagggcat actgttcgtg atcgccgctc cgtcgggaac ggggaagtcg
     4321 agcgtcgcgc gcgaggtgct ggcgcgggac gcggagctcg agttttcgat ctcgtacacg
     4381 accagggccc ctcgcgacgg cgagcgggac ggcagggaat atcatttcat cgatcgcgac
     4441 gagttcgcgc gcatgcgcga acgccaagcg ttcctcgaat cggccgaggt attcgaccag
     4501 ctttacggaa ccgggttgga ggcgacgcgt cgtagcctgg cgaccggaaa ggacctgctg
     4561 ttggacatcg acgtccaagg tgcgcgtcag gtgacgggcg gcccgctcga ggcggtgacg
     4621 atcatgatct tcccgcccga ctatcggacg ctgaccgagc ggctcgagcg gcgcggcagc
     4681 gaggatcggg cgcagtcgcg gcgccggctg gccaaggcgc gggccgaggc cgaagactac
     4741 ctttcgttcg actaccttgt cgtcaaccgc gacctcgaag cggcggtgcg cgacgtctcg
     4801 gccatcgtgc acggcgagcg ccgacgaacg cggcgctgcg ccaccgaggc gcggagggtc
     4861 ctcgccacct tcccggtcga gggttcgacc acggaatgac aaccgcccgg cgtgcggcgg
     4921 gcacgcccga aaaaggagac cgtccgatgt tcaagctccc ggaggatatc gacaacaagt
     4981 accgcttcgt gaccctggcg tcgaagcgtg ccgagcagct acagttcggc gcgctgccac
     5041 gtgtcgccga caccgcccgc aaatcgaccg tgatcgccca ggaagaggtc gcgtcggggt
     5101 tggtcgagtt gtgggcgccc gagcgagagg gcgcggaggg cgccgtcgcc gacgaggaag
     5161 aagaggaata ggtcggtctc gcgatgttga tcgcgctggg agtcagcggt gggatcgcgg
     5221 cgtacaagac gccggagatc gtccgcgcgc tcgaccgggc cggggccgaa gtgcaggtgc
     5281 tgctgacgca aaacgcctgc gagttcgtca cgccgttgac gttgcagacg ctgtcgcgaa
     5341 accgcgtgct gatcggccag tacgacctcg acgaggagca gacgatccgc cacatcgagc
     5401 tgacgcggcg ggtctcggcg ttggtcgttg ctccggcgac cgccaacttc ctggccaaga
     5461 tggcacgcgg cgtggccgac gatctgttgt cgacgttcta caccgcggtg accgcgccgg
     5521 tcgtcgtcgc cccggcgatg aacacccgga tgtggctgca tccggcgacg caggcgaatc
     5581 tcgctgtgct gcgcgggcgt ggcgtgacgg tgatcgagcc ggagtcgggt tggctcgccg
     5641 aaggcgagac cgggcaaggc cggatggccg agccggggga gatcgcccgc gccgcctggc
     5701 acgccggcct ccgatcgagc cagctcgccg gcaagaagat cgtggtcacc gcaggtccga
     5761 cacgcgagcc gatcgatccg gtccgattcg tctccaaccg ttcgtcgggc cggatgggtc
     5821 atgcgttggc cgccgccgcg gcgcgtcgcg gcgcgcgggt cgtgctcgtc agcggtccgg
     5881 tcgatttgcc gccaccgtac ggtgtcgagc gcgtcgtcgt cgagacctcg gccgagatgc
     5941 gccgggcggt gctcgacgcg cgcgaggggg ccgaggcggt gttcatggcg gccgccgtct
     6001 ccgatttcat tccgcgccgg agcccgtcga agatcaagaa acgagaggcg accctcacga
     6061 tcgagctcga gcgcggtccg gacatcctca gcgagctcgg ccaatcgaga tgcgagcgcg
     6121 tgctggtcgg cttcgccgcc gagaccgagt cgttggtcga gcatgcgcgc gagaaactcg
     6181 agcgcaagcg ggtcgacttc atcgtggcca acgacgtttc gcggcgggac atcggcatgg
     6241 aggccgacga caacgccgtg accattctct cccgcgacgg tgggatcgtc gaggtgccca
     6301 aggcgtcgaa ggcggagatc gccgacgtca ttctcgaccg cacgctgggt gcggtggttg
     6361 tcgaggagcc gtcgtgaacc cgacgagcgg agcgggtggc cggagcgcga cgcgcgagct
     6421 cgtcgagtgg gtcgactacc tgcgcgagat cggtgtgcgc gagctgtgtg tcggcgagac
     6481 cgggtcgccg gccgccgcgc ccggcgcgct cccggcgcgc gaggagggaa cgccgccgcc
     6541 gacccggccg gcggcaccgc ccgccccgac ccggcccgtg ctggccgcgc tcgccgtgtt
     6601 ctccggcccg ggctaccccg acccgggcgc gcgcttgcag cagatccgcg acgagctggg
     6661 agagtgtagt cggtgcaagc tgcacgcgac gcgcaaccgg atcgtgttcg gtgtcggcga
     6721 tccgcgcgca cggctgatgt tcgtcggcga gggacccggg gccgacgagg acgcccgcgg
     6781 cgagccgttc gtcggtcgag ccggcaagaa gctcgacgag atgatctccg ccatcggttt
     6841 gacacgggcc gaggtctaca tcgccaacgt cgtcaagtgc cggccgccga agaaccgcga
     6901 tccggagagc gacgagatcg cgacctgttc gccctttctc gagctgcaga tcgaggcgat
     6961 ccgcccgcgg gtgatcgtca cgctcggcgc tccggcggcg cgcacgttgc tgaacacgcg
     7021 cgtcggcatc acgcgtctgc gaggaaactg gcacagcttc cgtggcatcc cggtgatgcc
     7081 gacctatcac ccggcctacc tgctccgcgc ctacacgcgg gagaaccgcc tgaaggtatt
     7141 cgaggacctg aaagccgccc gcgcgcgcat ggacgcgtag ctcgcatggc gcaaccgcgg
     7201 tacgccgccg tcgccgtacc ggttcccgtt cgtcggctgt tcagctactc gatcgacgaa
     7261 gcgctggcgc cgcgcatcac gaccggcgtc cgcgttcgcg tgccgttcgg ccgacgcacg
     7321 ctggtcgcga cgatcgtcga gttcccggcc gcgcgcccgg cggccgacgt cgagccgaag
     7381 cccgtcctcg agatcgtcga cgacgcgatg ctcgatcctc gactgctcga gctgacccgt
     7441 ttcgtcgccg actattacct gtgctcgtgg ggcgaagcga tcgaaaccgc gctgccgccc
     7501 ggctgtggga ccgccggccc gcgggtggtg cggctcggcg acggggcgag cctcgaggcg
     7561 atcccccggc gcgcgacggc gcggcgccgt ctgttccgtc gcgtcgcgga gcgcgacgga
     7621 ccgctgtcgc tggcgtcgtt gggcacgaac gagcggcgat tgctgccgac gatggtcgaa
     7681 gacggcctgc tggtcgtcgt ccgcgggcag acgggcagcg accgcgagcc gcccgagccg
     7741 cggcatgcgc cgccggccga ggcgccgacg ccgacagcgg cgcaggcgcg cgtgctcgag
     7801 cgcatcgatc ccgcgatcga gcggcgcggc ttccggccgt tcgtcctgtt cggcgccacg
     7861 ggctcgggga agaccgaggt ctacttccgc gcggccgagc gcgcgttggc cggcggtcgc
     7921 ggcgtgctct acctcgtccc cgagatcgga ctgaccccgc tcttgctcgg cgccgtgcgc
     7981 cgcaggttcc gcggagaggt gacggtgctg cacagcgggc tgggccgtcg cgagcgccgg
     8041 gacggttgga acctcgtgcg caccggccgc agccggttcg tcatcggcac gcggtcggcg
     8101 gtgttcgcgc cgctggccga cattggcctg atcgtcgtcg acgaggagca ggacggctcc
     8161 tacaaacaga gcgagacgcc tcgctacaac ggtcgcgacc tggcggtcgt ccgcgcccat
     8221 gcgcacgagg cgacgatcgt gctcggctcg gccaccccgt cgatggagac cttccggcac
     8281 gcgcgcgcgg agcgctacga gctgctccgg ctgggcgatc gcatcgagcg ccgtccgctg
     8341 gccgacgtcc gcatcgtcga catgcgcgag gtctaccggg cacggggcga agtgacaccg
     8401 ctctcgccgc tattggccgg cgagctgcgc gactgcctcg agcggggcga ccaggcgctg
     8461 atcctgcgca accggcgcgg atgggccgct gcgctgcact gtccgtcgtg cggcgaccgg
     8521 gtcgagtgcc cgaactgcac gatcgcgttg acctggcatc gtgccgcgcg ccggcttcgc
     8581 tgccattact gtggtctcga gcaaggctat cccgacgcct gcccgcgctg cggcgcggcc
     8641 gagctgaacc cactcggcga agggaccgag cggatcgagg accggatcaa gcgcctgttg
     8701 cccgccgcgc gcatcgatcg gatggaccgc gacacgatcc gtcgccgcgg cgcacacgag
     8761 aagatgctga gacgcttcga gcgcggcgag atcgacgtgc tggtcgggac gcagatgatc
     8821 gccaaagggc atgactttcc ccgcgtcacg ctcgtcggcg tgctgtccgc cgaccaggcg
     8881 ctgggcatcc ccgacttccg cgcggccgag cgggcgttcc agctgttgac gcaggtcgcc
     8941 ggtcgcgccg gccgcggcgc gagtcccgga cgagtcgtgg tccaggcgtt cgatgccgac
     9001 cacccggtgc tggggttggc cgcgcgccag gactacgagg cgttctacga tcgcgagatc
     9061 cgttatcgtc gcgcactgcg ctaccccccg ttgtcggcgc tggtccagat catcgtgcaa
     9121 gacaaagacc cgatcacggc ccgcggctgg tcggagcgcc tggccgaggc actgcgggag
     9181 caggacccgg agcggctgtt gctctccggt cccggaccgg cgccgatcga gcggctgcgc
     9241 ggccgcgtcc gcgagcagat cctggtccgc ggcgccggcc ggcgccggct cgtcgagacc
     9301 gtcgaccgcg cgttgcgcgc cgtcgagggc cagatcccgc gccgggcgat cacggtcgac
     9361 gtcgatccac tgtcgttgct gtgaggtcgc gtgtcgccta acaccgtacc gcaagggcca
     9421 ctcgtcgtcg gatcgcttct cgcgagcgat gtcgcgaccg ccggcgcgat gctggccggc
     9481 gcgccgccgg gggctctgtt gctcgagctg cgcgccgatc ggctgcgggc ggacgaggtc
     9541 gccgcgatcg tgcgacggac cgaacgcccg ctgatcgtca cggtcagacg ctcggcggac
     9601 ggcggggggt tcgacggcag cgagcacgag cggcgcgccc tgttgaccgc ggcgctcgac
     9661 gccggcgcgc gtttcgtcga cgtcgaatgg gatcggccgc tcgcggcgct ggcggacgac
     9721 ccgtccctga ccgaacgcgt gattctgtcg catcacggtg tggcctgtcg ggtgcgagcg
     9781 ctcgagcggg tcggtcgagc gatgcgggcg cgcgacgccg cagcctacaa gatcgtcgcg
     9841 tcggcagcgg aggtcgacga gctgcccgcg attcgggccc tgctcgctcg ccggccgctc
     9901 gccggccggc cgctggcggc gttcgccgac gggcgggcgg gcgtgctcag ccgactgctc
     9961 gcgccgtcat ggggctcgtg ggcgacctac gccgcgctcg atcgaccgac cgccgagggc
    10021 cagttcggcg tcggcgagat gctcgatggc tacgacgtgc tcgcgatctc ggaccggacg
    10081 agacgctatg ccctggtcgg cagcacggtc tcgaacagcc cgtcgccggc gatgcacaat
    10141 gcggccctcc gccagctcgg cctcgacgcg cgttacttcg cggtcacgac cgaccgcttc
    10201 ggcgcgttcg tccgcctcgc gcgatcgccg gccgccgcgc tcgccgggtt cggcgtgacg
    10261 atgccgttca aggagatcgc ggcgcggcgc tcgcgccccg ccgactcgat cgcgcgcgcg
    10321 agcggcgcgg tcaatacggt cgtcgtcggg ccgggcggct gggtcggcta caacaccgac
    10381 ggtccggccg cgctggcgtc gatcggcgag cggatcgatc cggcgggttg ccgggtcgcg
    10441 gtcctcggcg ccggcgggac cggacgcgcc atcgcctgct cgctcgcggc tgccggtgcg
    10501 cgggtccgac tgttcaaccg cagcgccgag cgcgcgcgcc gggcggcgcg cgcactcggc
    10561 gtcgaatggg cacccgagga gcgattggac gaatggcgct gggatgtcct ggttcacgcc
    10621 acaccccgcg gagcgggtgg cgagcggttc atcgccccgg agcggttgcg tggcgcggtc
    10681 gtgctcgacg cggtttaccg gcctcgcgag acgatgctgt gcgccgacgc gcgccgggcc
    10741 ggtctgcagg tgatcgacgg ccgccggctg ctactggagc aggcgctgct gcagttccgc
    10801 ctgctgacca cgcacgacgc ccgtgtcgag acgctgcggg cagcggccgt cgcctggctc
    10861 gggtcggggt ggtgactcga cgcgaacccg gggcacgacc gggtcgagtc gcgcttgacg
    10921 gctgggccgg ctcggcctac cctcgatcat cgacgtcgtg gaaagggaag cgcatcgtgc
    10981 gcgtgatcgt ttgcactgcc tctgccgtcc tgctgctggt tttcgccggc tgtgccgcga
    11041 gacagccgat cgagggccag ccgacgacgg ccctcgaccg caagctgtcg actttcgcct
    11101 tcatcgagga aggcgatctg atcacgctga tcgtcggcac gcaggccgca cgttaccgcg
    11161 agaaatccga gttcatgccg gtcgaggtga tgatcgcgaa caacggtctg aaaaacctgg
    11221 tgctgacccg cgagtcgttc acgttgatcg acgagcaggg caaccgctat ccactggccc
    11281 gcccgcagga gctgtacgag ggctacgatt ttctcgacct cgaccgacag gcgctgtcga
    11341 cgctgccgga cgtgacgacc aacaagttcg ccgcctacac ccgctacggc agccggttct
    11401 ctccgacgcg ctcgaccgtg gccgacctga ccggcagcgg ttcgctggtg cgcgacgcca
    11461 tctcggtccc caagttcggc tacatctggg acttcctcta ctttcctcgt ccggtcaccg
    11521 gcatcaaggg ccaccgtttc gagctgttcg tcgaatcggc cggcctcgaa gacccggtct
    11581 tcgtcaagtt cgaagtcaag tgaagatggc ccgcctgctg ctgctcgaca actacgactc
    11641 gttcagctgg aacctggtcc agtacctcgc cgagctgggc gccgacgtcg acgtcaagct
    11701 gcacgacgag gtcgacgtgt cgtggtgcgt ctcgcagcgg ttcgatgcga tcgtcatctc
    11761 gccggggccg ggccgaccgg aaggcgcggg gatcagcatg gacctgatcc gcgaggtcgg
    11821 cggcagcgtg ccgctgctcg gcgtttgcct cggccaccag gcgatcgcgc gagtctacgg
    11881 cgccgagatc atcggcgcgc cgacgctgat gcacggcaag acctcgccga tcgtccacga
    11941 cggccgggga atcttcgccg ggctcacccc gtcgttcgag gccacgcgct accactcgct
    12001 ggtcgtcgac gagtcgacgc tcgatccgac acggtttctc atcacggcgc ggacgcccga
    12061 aggcgtgatc atggcgatcg agcaccgccg ctttcccgtc ttcggcgtgc agttccaccc
    12121 cgaatccgtc ttgacgctcg aaggaaaacg gctgctcggt aattttctcg gactcgccgc
    12181 ggcgaatcgc gaacaggcgg tctgaacgag aggagggttc gacatgcagc agctctcgcc
    12241 gtacaacctg aagcagtgga tcgaggagaa tcgacacctg ctcaagccgc cggtcggcaa
    12301 caagatcatc tggcaggatg gggagttcct ggtgatggcc gtcggtggtc cgaaccgccg
    12361 caaggacttt cacgtcgagg acgggcccga gttcttctac cagatcgagg gcgacatcac
    12421 gctgcggatc atcgaggacg gcgaaccgcg cgacatccag attcgcgagg gtgagatctt
    12481 cctgcttccg ccgcggatcc cccattctcc gcaacgaccg gccggcacgg tcggcttggt
    12541 catcgagcgc gtacgcaaag cgggggagcg ggaccatctc gtctggtttt gcgaatcgtg
    12601 cggcgacacg ctacacgacg ccgcgttcga gttgacggac ctgggcaccc agctcaagcc
    12661 gatcatcgag cggttctacg gcgaccagaa gctccgcacc tgcggcagct gccagacgac
    12721 gatgcaggtg cccaaggtgt gaggccgctg aagatcgacg tgcacacgca cgtgctgccg
    12781 cgctcgtggc cggacctcgc cgagcgttac ggttatcccg gcttcgtccg cctcgagcac
    12841 gacggaccgg accgggcacg gatgatgcgc gaaggcgagt gtttccgcgt gatcgagtcc
    12901 aactgctggt ccgccgaacg ccgcctcgag gagtgtgacc ggaccggggt cgacgtgcag
    12961 gtgctgtcga cggtgccggt gatgttcagc tactgggcca gaccggagca tgcgctcgat
    13021 ttatccgcgc tgctgaacga ccacatcgcc gagttggtgt cgcgccatcc gcggcgcttc
    13081 gccgggctgg cgacggtgcc gctgcaggat ccggagcggg cgatcgccga gctcgagcga
    13141 gcggtcggcc agctcgggat gtgcggggtc cagatcggca gccacgtcaa cgactgggag
    13201 ctcgaccaac ccgagctgct gccgttcttc cggcgggcgg ccgagctcgg cgccgccgtc
    13261 ttcgtccacc cgtgggagat gctggccgcg gagcgcatga gccgctactg gctgcgctgg
    13321 ctcgtcggta tgccggccga gaccacgctc gccatctgct cgatgatctt cggcggcgtg
    13381 ctcgagcggc tgccgcgcct gcgggtcggc ttcgcccacg gcggcgggtc gttccccgcg
    13441 accgtcggtc ggattcaaca cggcttcgag gtgcggcccg acctgtgcgc ggtgcgcaac
    13501 gacgtctcac ccgccgacta cctgcgacgc atctactacg actcgctggt gcaccgcgcg
    13561 gacgcgttgc actacctgat ccgcaccgtc ggcgtcgacc gcatcttcct cggttcggat
    13621 tatccgtttc cgctcggcga gcggctgccg ggcgcgttga tcgaacggct cgaagggatc
    13681 gacgagaccg accgcgtccg attgttgtcg gggacggccc tcgagtttct cgggcgcgaa
    13741 gtcgccgact ttggctaaca tggcgggtgt gagcccgcga attttccacc cgggagaaga
    13801 gctggcgcgc gagctggacg cccgcgaccg actcgccgcc ttccgtgatc gattccacct
    13861 cccgccggac gacgacggct cgcccttggc ctacctctgc ggcaactcgc tcggtctcca
    13921 gccgcgcgcg acgcgggcca gcctcgagcg cgagctcgag gactgggcgc ggctggccgt
    13981 cgatgcccac ttcgacggac gaacgccgtg gtacagctat cacgagacgc tgcgcgggcc
    14041 gttggcccgg ctggtcggcg cgctgcccgg cgaagtcgtc gcgatgaaca gcctgacggt
    14101 caacctgcac ctgttgatgg tgtcgttcta tcgtccgacg cccgagcggc accgtatcct
    14161 gatcgagcag cacgccttcc cctcggacaa ttacgccgtg cggtcacagg cccggttcca
    14221 cggtttcgac cccggccaag cggtgatcgt cgcgcgcgcc cgcgacggcg agacgacggt
    14281 ccgcagcgag gacctcgagt cgctgatcga acgcgagggt ccgacgatcg cgctgatcct
    14341 gatcggcggc gtgcagttcc tcaccggcca gttgttcgat ctggcgcgga tcgccgagtg
    14401 tggccggcga cagggcgcga cggtcgcctt cgacctggcg cacgccgtgg gcaacgtgcc
    14461 gctggcgttg cacgactggg gagtcgactt cgcggtttgg tgcagctaca agtagctgaa
    14521 cgccggaccg ggcgcgttgg ccggttgctt cgtccaccgc cgccaccacg gcaagcagct
    14581 gccgcggctc gccggctggt ggggcaacga tccggcgacc cgcttccgca tgcacctcga
    14641 gccggaattc gtgccggtgt cgagcgccga tgcctggcag ctcagcaacc cgccgatcct
    14701 cgccgccgcc ccgctgcggg actcgctggc gctgttcgac gaggccggca tggcggcgtt
    14761 gcgcgacaag tcgctggcgc tgaccgccta cctgcaccgc ctcgtcgcgg aggtcgccga
    14821 ccggcgactg cgactgctga ccccggacga tccggccgca cgcggctgtc aaacctcgat
    14881 cgaggtcgcg cgcgacggcc gtgcgctgtt cggcgcgatg cgccggtgcg gcgtcgtcgg
    14941 cgatttccgc gagcctggcg tgatccgcgt cgcgccggtg ccgttgtaca actcgttcca
    15001 cgacgtctgg cgcttcggtc gcgcattggc cgaatggtcg actgcccggt gagcggtcgg
    15061 ggcgcgacgg gccgcaagga aaccgtgctg gtcgccggcg cggggctcgg cggcgcgttg
    15121 atcgcgatct acctcgcgcg cgccggtcac cgcgtgcgct tgttcgagcg ccgctccgat
    15181 ccgcgcaagc tgaccggcga ccgcggccgc tcgatcaatc tcgcgatctc gacccggggt
    15241 ctcgccgcgc tgcgcggcgt cggcgtcgac ggaccggtgg tcgagatggc cgtgccgatg
    15301 cgcggacgga tgatgcacgg cgtcgagggt cgattgacgt tccagcccta cggcacgacg
    15361 gggcaggcga ttcactcggt gtcgcggctc gggctgaaca tcgccttgat cgacgaggcg
    15421 gagcggcacc ccgaggtcgt catcgagttc gacagccgct tggtcgacgt cgatccggac
    15481 ggtcccgagc tgctgctcga gtcgcccgac gggagcacgc ggcgcgtcgc gggcgaccgc
    15541 ctgatcggag ccgacggggc gtattccgcc gttcgggcgc gcctgcagcg tctcgatcga
    15601 ttcgactaca gccagagcta tctcgagcac ggctacaagg agctgacggt ccccccggcc
    15661 gacgacggcg gtttccgcct cgagccggag gcgttgcaca tctggccccg cggcggcttc
    15721 atgatgatcg ccttgcccaa cgtcgaccgg tcgttcacct gcacgctgtt ctggccgctc
    15781 gagggagcga cgagcttccg ccagatcgac gacgcccctg gtctgcgcgc gttcttcggc
    15841 cgctggttcc ccgacgcggt cccgttgatg ccgcgtctgg ccgaggacta tttcgccaac
    15901 ccgaccagtt cgctggtcac ggtacgttgc cacccgtggc accacctcga ccgcgtcgtg
    15961 ttgttgggcg acgcctgcca cgcgatcgtg ccgttctacg gccagggggc caacgccgca
    16021 ttcgaggact gcgtggtgct cgatcgcgcg atccaccagc acgcgccgga ttggggcgcg
    16081 gcgttcgcgg cctacggtcg acagcgcaag ccgcacgtcg acgtgctggc cgatctggcg
    16141 ctcgagaact tcatcgagat gcgggatcgg gtcgcgtccc ggtcgttctt gtggcaaaag
    16201 cggctcgaga agggtctgca ccgcgccttt cccgagcgtt tcgtgccgct gtacagcatg
    16261 gtgaccttct cgcagctgcc ctacggcgac gcggtcgagc gcgcgcgccg ccagtggcgg
    16321 ttggtgcgcc ggatcgcggc caccgcggcc gccgcggcgc tgatcgcggc gggcttggtt
    16381 ctcggtctga tctggtagcc aaggagcggc gatgagcgac ccgacgatga actactcgag
    16441 ctacctcgag ctggaccgct tgctgtcgct gcaaagaccg cgctcgcgac cggtcgaaca
    16501 cgacgagctg ctgttcatcg tgattcacca ggtctacgag ctgtggttca agcagctgct
    16561 gcacgagatc gacaaggtca agctcgactt ctcggccggc gagttgttcg cggcgatcgc
    16621 cactttcaag cgcatgcgga cggtgatgaa aacgctggtc gggcaactcg acatcctcga
    16681 gacgatgacg ccgctgtcgt tctcggcgtt ccgcagccgg ctggagacgg cctccggctt
    16741 ccaatcgctg cagtatcgtg aggtcgagtt cgcgctgggc tacaaacgac aatcggtgat
    16801 gcgctacatc gattcgtcgt ggagcggcat cgagcgggtc gagcggagac tcggcgagcc
    16861 gtcgctggtc gatcacttct acgatttcct cgagcgacgc gcggtgacga ttcccgaggc
    16921 ggtcaagcga cgccgcggta cgccgcagcc gacgctgccc gacgagacgg tgcagcgcgg
    16981 actgctcgag ctgtaccacg gctcgcccga agcggcgatc ctgttcgagc tgatgatcga
    17041 cttcgacgag gggctgcagg agtggcgcta ccgccacgtc aagatcgtcg agcgcacgat
    17101 cggcagcaaa caggggacgg gtggaagtcc gggtgtcgag ttcctgaaga aatcgctgtt
    17161 cctgtcggtg tttcccgacc tgtgggcgat ccgccacgag ttctgagatg aagttgtacg
    17221 acatctcgcc caccgtctcg ccgtcgctcg cggtctggcc cggagacaca ccgccgcgac
    17281 gtgaagtgct gctcgacatc gatcgcggcg acaacctgac gctgtcgacg ctgcacgcca
    17341 cggtccacct gggggcccac gccgacgccc cgagccacta tggcgcaggc gcgccggcga
    17401 tcgaccgccg gccgctcgat gcgtacctcg gtcgctgtca ggtggtccgg gtcgacgtcc
    17461 ggtccggctc gcggctcggc gcggcggccg taccgggctc gcccgctgcg cggctgctgt
    17521 tcgccaccgg cagctatccc gaccccgacg agttccgccg ggacttcgcc gcgttgtccg
    17581 tcgagctgat cgacaccgcc caccggcggg gcgtgcgctt gatcggcatc gacacgccga
    17641 gcgtcgacct gttcgagtcg aaggagttgc cggcgcacca cgcctgcctg gcgcgggacg
    17701 tcgcgatcct cgaagggctc gtgctcgacg acgtgccggt aggcgtctac gagctgatcg
    17761 ccctgccgtt gaagctggcc ggtttcgacg ccagtccggt ccgcgccgtg ctgcgctcga
    17821 tcgactgatc ccgcgccggt tcgcgtcgga gggtcagagc cggtcgagcg cgtcggccaa
    17881 cgcggcgatc aggtcctcgg catcttccag tccgaccgac agccggacca ggccgtcggt
    17941 gatgccgacg cgtttgcgct cctgggcctc gacgtcggca tgggtcatcg tcgcgggatg
    18001 ggtcacgagc gactcgatcg aaccgaggtt ctcggccagc gtccacagct cgacggcgtc
    18061 catcagcttc ttgccggcgg cgacgccgcc gcgcacctcg aaccagacca tcgcaccgaa
    18121 gccgctcgcc tgcttgcgcg ccagcgcgtg ctgcggaaac gactcgaggc cgggatagca
    18181 tacgcggtcg accttcggct ggcgttccag gtactgcgcg accgccatcg cgttggccga
    18241 ctgccggtcc atccgcaccg acaaggtctt gcagccctgc aacgtcagaa acgccacctg
    18301 cggcgaaacg atcgtgccgg cggcgttctg gatgaagcgc agctggccgt cgagctccgc
    18361 ggtacgcgcg acgacggcac cgccgaccgt ggcgttgtgt ccgtcgatgt acttggtcgt
    18421 gctgtgcacc accaggtcgg cgccgagttc gagcggccgc tggtagtacg gcgtgagaaa
    18481 ggtgttgtcg acggcgtgga gcacgttgcg ctcgcgcgcg atctcggaga tcgcggcgat
    18541 gtcggtcagc ttgagtgtcg ggttggccgg cgtctcggtc aggatcaatc gcgtgttgtc
    18601 gcgaacgctg tcgcgcaccg ccgcgggcga ggcggtgtcg gcgaaggtga actcgacccc
    18661 gaaccgcgcg agcaccttgg tgcacagccg gtaggtcccg ccgtaggcca cgtcggacac
    18721 caccgcgtgg tcacccgcgt tgagcgtggc gagcagcacg gcctggatcg ccgccatccc
    18781 cgtcgaaaag cagctgcagt ccgccccgcc ttcgagcgcg gtcaacttgc gctccagcgc
    18841 gcgcaccgtc gggttcccgc tgcgcgagta ggaatagccc ttggacaggt actcgtcgac
    18901 ggacggctg
//
LOCUS       DASPGN010000515         5436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_447769, whole genome shotgun sequence.
ACCESSION   DASPGN010000515 DASPGN010000000
VERSION     DASPGN010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447769"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VD788_14995"
     CDS             complement(<1..701)
                     /locus_tag="VD788_14995"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HXV77618.1"
                     /translation="MTQEKSKRITPRGEDYSRWYTDLVIQGRLADYSPVKGCMVIRPH
                     GYALWENMQRELDRRFKDTGHQNAYFPLFIPKSFLAREAKHVEGFAKECAIVTHSRLM
                     SVEVDGRVDLVPDPQSALEEELIIRPTSETMIYSMFAKWVQSYRDLPLLINQWANVVR
                     WEMRTRLFLRTTEFLWQEGHTAHATAEEAEFETRQMLDIYAAFAEEVMAMPVVRGVKS
                     ESEKFAGAVRTYSIEA"
     gene            complement(738..1502)
                     /locus_tag="VD788_15000"
     CDS             complement(738..1502)
                     /locus_tag="VD788_15000"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXV77619.1"
                     /translation="MVHGTARYASGVGLFLAVLTMACAAGDGVGAGSGPREGDERNDE
                     FRSARAEMVATQIAARGVANQAVLEAMRRVPRHRFVPLELRNQAYYDSPLPIGHRQTI
                     SQPYIVALMTELIEPKPGMRVLEIGTGSGYQAAVLAECVDTVYTIEILPELGREAAEL
                     LDGLGYDNVVTRIGDGYDGWPEQAPFDAIVVTAAPASIPKPLLDQLAVGGKLVIPVGE
                     SIQDLVVVTRTEDGYVRRPASGVRFVPMTGKAQEQH"
     gene            1511..2221
                     /locus_tag="VD788_15005"
     CDS             1511..2221
                     /locus_tag="VD788_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77620.1"
                     /translation="MITVSIGSVRCQASRTRPRPARPVSLFLLTAAAALVGCGPGERE
                     ARETIGAFLAGVQNQDRARLYCLMTGARDADELAVDPADRRAAFEQWVGGMLEAYDWG
                     RDHGQVELDEHGVVASKLFALGKGTFFELSQVVHPAEDVMRVRMELRFGYAAVDLSGF
                     SPGTTFYVCGVPLGRVHAIRKPPGGGEESADVLDRLTLEWTLVRSPAGEVCPAGWTVA
                     TVVPLPETIATKRITWVF"
     gene            2221..3189
                     /locus_tag="VD788_15010"
     CDS             2221..3189
                     /locus_tag="VD788_15010"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXV77621.1"
                     /translation="MRSRFKFRVGVALGGGAARGLAHVGVLRALARAEIPVDIVVGTS
                     MGAIIGGAYAAIGDVGLLEQRVREVLESDEFRKNRLQFLKETRAQRGGLFFSMANLVR
                     RGIFFGVSNLRPSFLSAEEFANSMEAMIPDEQIEELPIVFGAVALDIQSAEEIVLRTG
                     SLRQAAAASSAIPGILPPVHMNGRKLIDGGWVDKIPVLPTYYLGADVVIAVDITADLQ
                     DAREYRRGVDIMVRANSIKDATLVDFSRRMADVVIAPEVGDVHWADFGDFDRCIQAGD
                     DATAEVVDRLRELTRQERLLSIVRRTPGRKLAELHLKASVHQLTVE"
     gene            3210..4634
                     /gene="mpl"
                     /locus_tag="VD788_15015"
     CDS             3210..4634
                     /gene="mpl"
                     /locus_tag="VD788_15015"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HXV77622.1"
                     /translation="MARSRGSHIHLLGIGGTGMTALAGLLHQQGCRVTGADGPLYPPT
                     STILEHLGVEVYHGYDPAHLDPAPDLVVIGNAISRGNAELEAVLDAGLDYRSMPQTIA
                     ERFLVGRHSMVVSGTHGKTTTSSMLATVLATAGRDPSFLIGGVPLDFGVPYRLGRGEE
                     FVIEGDEYDTAFFDKGPKFMHYRPRTVLLGTVEFDHADIFADLEAVRTAFRRLINLIP
                     RSGLLACHMDSPVVRELADGGRCRVEGYGFEHGWWRAVDLVDSTDGVRFRVLRDGRAY
                     ARIEMQVPGKHNALNALSVVVAAADRGLAPAEIARGLAAFRGVRRRLERRADTGGVLV
                     LDDFAHHPTAIANMLEAVRRAHGGRRIWAVLEPRSWSLRRNVFQTQLAAAFDHATKVV
                     VARVYGEDALAERERLDPVRLVADLTKRGRDAVHLGQTSQIVEHLVEGTRRGDVIVVM
                     SNGGFDGLLDKLIPALEARGIKAS"
     gene            4631..5302
                     /locus_tag="VD788_15020"
     CDS             4631..5302
                     /locus_tag="VD788_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HXV77623.1"
                     /translation="MSINRPKRLWIIDPSMAVAETQGIAEVVGEWAGEHRVFQPALCP
                     GDGPRPGDGYDTDGVVLLGSRASVFDRLGWLTELGDWIAPIVAGRVRLPLLGICFGHQ
                     LIAHVAGARVAPLSDDGAKRVGVETSILEGSRLVPGRVEIPVVVSHREIVRACPERFQ
                     VVARRQSVPIDGIEHDELPVFAFQFHPEARDEFAVRVGIEPRRIDDRLRVANRRLLAA
                     FRDRV"
BASE COUNT          801 a         1799 c         1912 g          924 t
ORIGIN      
        1 gcttcgatcg aataggtgcg caccgcgccg gcgaactttt cgctctcgct cttgacaccc
       61 cgcaccacgg gcatcgccat cacctcctcg gcgaatgcgg cgtagatgtc gagcatctgt
      121 cgcgtttcga actccgcctc ctcggccgtg gcgtgggccg tgtggccttc ctgccagagg
      181 aactcggtcg tgcgcaggaa caatcgggta cgcatctccc agcgcacgac gttggcccac
      241 tggttgatca acagcgggag gtcgcggtag gactgaaccc acttggcgaa catcgagtag
      301 atcatcgtct cggaggtggg gcggatgatc agctcttcct cgagcgcgct ttgcgggtcg
      361 ggcacgaggt cgacccggcc gtcgacctcg accgacatca gcctcgagtg cgtgacgatc
      421 gcgcactcct tggcgaaccc ttcgacgtgt ttggcctcgc gggccaggaa cgacttgggg
      481 atgaacaacg ggaagtacgc gttctggtgc ccggtgtcct tgaaccgccg gtccagctcg
      541 cgttgcatgt tctcccacaa ggcgtagccg tgggggcgga tgaccatgca gcccttgacc
      601 ggcgaatagt cggccagccg gccctggatc accaggtcgg tgtaccagcg cgagtagtcc
      661 tcgccgcgcg gtgtgattcg cttcgatttc tcttgagtca tcggttccac ctcgcccggc
      721 gcgcgggaag gacgcggtca gtgctgttct tgtgccttcc ccgtcatcgg cacgaagcgg
      781 accccggagg ccggccggcg gacgtagccg tcctcggttc gcgtcacgac gaccagatcc
      841 tgaatcgact cgccgacggg gatcaccagc ttgccgccga cggcgagttg gtccaacagc
      901 ggcttgggga tgctcgccgg cgcagcggtg acgacgatcg cgtcgaacgg agcctgctcg
      961 ggccagccgt cgtagccgtc tccgatccgg gtcacgacgt tgtcgtagcc caggccgtcg
     1021 agcagctcgg ccgcctcgcg accgagctcg ggcaggatct cgatcgtata aacggtgtcg
     1081 acgcattcgg cgagcaccgc ggcctgatac cccgaaccgg tgccgatctc gagcacacgc
     1141 atgccgggct tcggctcgat cagctcggtc atcagcgcga cgatgtaggg ttgtgagatc
     1201 gtctgccggt gaccgatcgg cagcggtgag tcgtagtagg cctggttccg cagctccagc
     1261 ggaacgaacc gatgccgcgg gacgcggcgc atcgcctcga ggacggcctg gttggcgact
     1321 ccacgtgccg cgatctgcgt cgcgaccatc tcggcacggg ccgagcggaa ctcgtcgttc
     1381 cgctcatcgc cctcccgcgg gccgctgccc gcgccgactc cgtctcccgc tgcgcatgcc
     1441 atagtgagga ccgccaggaa cagcccgaca cccgacgcgt accgtgcggt tccgtgtacc
     1501 atcgtcacgc atgataaccg tatcgatcgg ttcggtccgc tgtcaagcgt cgcggacccg
     1561 accacggccg gccaggccgg tttccctgtt tctactcacc gcggccgccg cgcttgtcgg
     1621 ctgcgggccg ggtgaacggg aggcgcgcga gacgatcggg gccttcctgg ccggcgtaca
     1681 gaaccaggac cgcgcccggc tctactgtct gatgaccggt gctcgcgatg ccgacgagct
     1741 ggcggtcgac ccggccgacc ggcgcgcggc tttcgagcag tgggtcggcg gaatgctcga
     1801 ggcgtacgac tggggtcggg accacgggca ggtcgagctg gacgagcacg gggtcgtggc
     1861 gagcaagctg ttcgcactgg gcaaagggac gttcttcgag ttgagccagg tcgtgcaccc
     1921 ggccgaagac gtgatgcggg tgcgaatgga gctgcgtttc ggctacgccg cggtcgactt
     1981 gtcgggattc tcgccgggaa ccacctttta cgtctgcggc gttccactgg gtcgcgtgca
     2041 cgcgatccgc aagccgccgg gagggggaga ggaatccgcg gacgtgctgg atcggctgac
     2101 gctggagtgg acgctggtgc gcagcccggc gggcgaggtc tgtccggccg gctggacggt
     2161 cgcaacggtc gtgccgctgc ccgagacgat cgccaccaag cggatcacct gggtgttctg
     2221 atgcgcagcc gcttcaagtt ccgtgtcggc gtggcgctgg gcggcggcgc ggcgcgaggc
     2281 ctggcgcacg tgggcgtgct gcgtgcgctc gcccgggccg aaatccccgt cgacatcgtc
     2341 gtcgggacga gcatgggggc gatcatcggc ggcgcgtacg cggcgatcgg tgacgtcggc
     2401 ctgttggaac agcgcgtgcg cgaggtactg gaaagcgacg agttccgcaa gaaccggttg
     2461 cagttcctca aggaaacccg ggcgcagcgc ggcgggctgt tcttctcgat ggccaacctc
     2521 gtccggcgtg ggatcttctt cggagtctcg aacctgcgtc cgtcgtttct gtcggccgag
     2581 gagttcgcca acagcatgga ggcgatgatc cccgacgagc agatcgaaga gctgccgatc
     2641 gtcttcgggg cggtcgcact cgacatccag tcggccgaag agatcgtgct tcgcaccggc
     2701 agcttgcgcc aggcggctgc cgccagctcg gcaatccccg ggatcctgcc gccggtccac
     2761 atgaacggtc gcaagttgat cgacggtggg tgggtcgaca agatcccggt gctgccgacc
     2821 tactacctcg gcgccgacgt cgtgatcgct gtcgacatca ccgccgacct acaggacgcg
     2881 cgggagtacc gccgcggcgt ggacatcatg gtccgcgcga actcgatcaa ggacgcgacg
     2941 ctggtcgact tttccaggcg gatggccgac gtcgtcatcg ccccggaggt gggcgacgtc
     3001 cactgggcgg acttcggcga tttcgatcgt tgtatccagg ccggtgacga tgcgacggcc
     3061 gaggtcgtcg atcggctgcg cgagctgacg cgacaggagc ggctgctgtc gatcgtgcgg
     3121 cgtacgccgg gccggaagct ggccgagctc catctcaagg cgtccgttca ccagctcacc
     3181 gtagaatagt gggacgggag gcaacgccgt tggctcgttc acgcggatcg cacatccact
     3241 tgctcggcat cggcggtacc gggatgaccg cgttggccgg gctgctccac cagcagggct
     3301 gccgcgtcac cggggcggac ggaccgctct atccgccgac gtcgaccatt ctcgagcacc
     3361 tcggtgtcga ggtctaccac gggtacgatc cggcgcatct cgatcccgca cccgatctgg
     3421 tcgtgatcgg caacgcgatc agccgcggca acgccgagct cgaggcggtg ctcgacgccg
     3481 gtctcgacta tcgatcgatg ccgcagacga tcgccgagcg gttcctggtc ggacggcatt
     3541 cgatggtcgt ctcggggacc cacggcaaga cgaccacctc gtcgatgctg gcgaccgtgc
     3601 tggccaccgc cgggcgggat ccgagcttcc tgatcggcgg cgtcccgctc gatttcggcg
     3661 tgccctaccg cttggggcgc ggcgaggagt tcgtgatcga gggcgacgaa tacgacacgg
     3721 cgttcttcga caagggaccc aagttcatgc actaccggcc gcggacggtg ctgctgggta
     3781 cggtcgagtt cgaccacgcc gacatcttcg ccgacttgga ggccgtgcgc accgcgttcc
     3841 ggcggttgat caacctgatc ccgcgctccg gtttgctcgc ctgccacatg gacagcccgg
     3901 tcgtgcgcga gttggccgac ggtggccggt gtcgggtgga aggatacggc ttcgagcatg
     3961 gatggtggcg ggccgtcgac ctggtcgact cgaccgacgg ggtgcgtttc cgcgtcctgc
     4021 gcgacggacg ggcctacgcc cggatcgaga tgcaggtccc cggcaaacac aacgcgctga
     4081 acgcgctttc cgtcgtggtg gccgccgccg accgcgggct cgcgccggcc gagatcgcgc
     4141 gggggctggc cgcgttccgc ggcgtccgcc ggcggctcga acggcgcgcc gacacgggcg
     4201 gggtgctggt cctcgacgac tttgcccacc atccgacggc gatcgccaac atgctcgagg
     4261 cggtgcgccg cgcgcacggc ggccgccgga tctgggccgt gctcgagccc cgctcctggt
     4321 cgttgcgtcg gaacgtcttt caaacgcagc tcgcggccgc cttcgatcat gcgaccaagg
     4381 tcgtcgtggc ccgggtctac ggagaagacg cactggccga acgagaacgg ctcgaccccg
     4441 tgcggctggt cgccgacctg accaagcgtg ggcgtgacgc cgtccatctg ggccagacca
     4501 gccagatcgt cgagcacctg gtcgagggta cgcgtcgtgg cgacgtgatc gtcgtgatgt
     4561 cgaacggcgg cttcgacggc ttgctcgaca agctgattcc cgcgctcgag gctcgtggaa
     4621 tcaaggcatc gtgagcatca accgccccaa gcggttgtgg atcatcgatc cgtcgatggc
     4681 ggttgcggaa acccaaggca tcgccgaggt cgtcggtgag tgggcgggag agcaccgcgt
     4741 gttccagccc gcgctgtgcc ccggcgacgg gccgcggccg ggcgacgggt acgataccga
     4801 cggcgtcgtc ctgctcgggt cgcgggcttc ggttttcgac cggctcggct ggctcaccga
     4861 gctgggcgac tggatcgcac cgatcgttgc cggccgggtt cgcctgccac tgctcgggat
     4921 ctgcttcggt catcaactga tcgctcacgt cgccggcgcg cgagtcgctc cgctgtcgga
     4981 cgacggtgcc aaacgggtcg gcgtcgagac gtcgatcctc gagggaagcc ggctcgttcc
     5041 cggtcgggtc gagattcccg tagtggtcag ccatcgcgag atcgtgcggg cctgcccgga
     5101 gcgcttccag gtggtggccc gccgccaatc ggtcccgatc gacgggatcg agcatgacga
     5161 gctgccggtg ttcgcgtttc agttccaccc cgaggcgcgc gacgagttcg cggtgcgcgt
     5221 ggggatcgag ccgagacgga tcgacgatcg actgcgggtg gcgaaccgcc ggttgctcgc
     5281 cgcgtttcgc gaccgcgtct gagcgatcac cgctttccgc cctggctctt cgaccgcgag
     5341 cccgcccggc cggagtcccc cgagcgggac gatggacgcc ccccggcggg ggccctgccg
     5401 ccgcccgacg ggcggctgac gcgcgagggg gcgccg
//
LOCUS       DASPGN010000516         5092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1618771, whole genome shotgun sequence.
ACCESSION   DASPGN010000516 DASPGN010000000
VERSION     DASPGN010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5092
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1618771"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VD788_15025"
     CDS             <1..482
                     /locus_tag="VD788_15025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXV77624.1"
                     /translation="LIAELVGPDRIVEGVRLFRERYAEVYTDGTRVLTGVRATLADLT
                     ARGYRLSVASNKPARFGSEIARRLALSDYLVSVHGPDTVGVTKPHPAMLERCLADMGS
                     TATHGLYVGDMVLDVESGARAGLPVVLVSSGSSSHADLQATGCLTIGSLEELRAILP"
     gene            complement(487..1974)
                     /locus_tag="VD788_15030"
     CDS             complement(487..1974)
                     /locus_tag="VD788_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77625.1"
                     /translation="MLQAVFVLGTQYRFGEGPLAVDTDSRWFASLPPDGEARAVLELR
                     RWPGDTVLSLYALPAGEPGDAYVPPVPGAGLARLSEDDPAEDAWDGSLETAAGRRPVL
                     WIERVLGPDQPRLGALLLPGDGGLGQEAVASLTDELFAVLRRVEVREAAWKAGRPVPP
                     GVPLSLPLTHETPGESSEEEAPWQVVRARDFTIGLPPGFRARRMDGDVPPPRQLDGGR
                     LWFRGRTRDSDGQIVAVGDGNRFGYVARVDTLAGWDDGKLAPLPLRGRPVERQAMESF
                     DLAAERSGASACAAERWTEPGFAGEWLLFRLKFTDHGYEIGLPVLAGTRSTSLYWIPA
                     TWRDERSAPAPPPIDPAERFGIKFERLTRADRQKQPWVEGYLSTPGLRAEVSLGISPA
                     ASLRSVDGYPIRFLSEEGDEVGSLVRLAAEEVETERESLAALTESDKPGRHRAARVLR
                     DEADNWLFLAPAGHGFLFEFTGGTSERSAELLELWELMIRSVRLQ"
     gene            complement(2163..3044)
                     /locus_tag="VD788_15035"
     CDS             complement(2163..3044)
                     /locus_tag="VD788_15035"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXV77626.1"
                     /translation="MRIKLATVAFIVGCAMWLPVSSGERERDREESRGEARAWLGVWL
                     SDAVDGGAQVNAVVGGGPADLAGLRSGDVFLEANGTAVGNEGDLGRVLRELVPGDALR
                     LKGLRAGQAIEWTATLGDRRSRRETVPEPWVVPLPTRPLLPPQPTVWTVGRVGLEVEE
                     ITPALRQHFGAPAGAGVLVTRVDAGGAAAEAGVEVGDVVVRVGETEIRTERDLRGNLS
                     SWNRRAPFIVEVVRARKPAQLEVVPPVVTEAPAPPPADSGPEGHTAHALELQIERLER
                     RLEEMKRELERVRREKN"
     gene            complement(3117..4241)
                     /locus_tag="VD788_15040"
     CDS             complement(3117..4241)
                     /locus_tag="VD788_15040"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HXV77627.1"
                     /translation="MAGSYVWCTRTEDLAREIGRIGSGPIALDSESDSLHHYPEKICL
                     VQLSSAGRDLLIDPLAGVDLEPLGPLLAERRIRKVLHGADYDLRVIDRDLGLRVAGLF
                     DTMVAARLCGERQFGLAALLDRYLGVQLDKRFQRADWSRRPLPAAMIRYAVLDTRHLV
                     ELSSRLESRLQALGRITWAEEEFGVLERIRFSEPDDREAYRAVRGSATLDRRGLAVLR
                     ELHVLRERLARAADRPPFRIAHNELLLRIARERPRSRERIAQLLDRRESAGASGAAAR
                     WSEAVERALSLGEEELPAPTALDVGRRVARKSARLKWLVERRNGVAVELGLDPSLVGP
                     RAVVEAALERADRGENPQDTPGLRRWQWALIGEAILDAAE"
     gene            4367..4924
                     /locus_tag="VD788_15045"
     CDS             4367..4924
                     /locus_tag="VD788_15045"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HXV77628.1"
                     /translation="MTRIAMCMMLPLLIPSVVVAGEVAGVAMPDAVTVDGVELTLNGI
                     GLREKLWVDVYVAGLYLERASDDARQIIDSEQTKQLQMQFVYKVGKQKLTGAWTAGLE
                     ANQPNRFVELTEQLNRLNAMMDDVADGDVMRFTTRPGQGLRVEVKGVDKGLIEGDEFA
                     RAFWSIFLGDKPPTEKLKSGLLGGS"
BASE COUNT          757 a         1865 c         1742 g          728 t
ORIGIN      
        1 tgttgatcgc cgagctggtc ggacccgacc gcatcgtcga aggtgttcgt ctgttccgcg
       61 agcgctacgc cgaggtctac accgacggaa cacgcgtgct gaccggcgtc cgcgcgacgc
      121 tcgccgatct caccgcccgc ggctaccggc tgtccgtcgc gagcaacaaa ccggcgcgat
      181 tcggcagcga gatcgcccgg cgtctcgcgc tgtccgacta cctggtatcg gttcacgggc
      241 ccgacacggt gggggtgacg aagccgcacc cggcgatgct cgagcgctgt ctcgccgaca
      301 tgggatcgac ggcgacccac gggttgtacg tcggcgacat ggtgctcgac gtggaaagcg
      361 gcgcgcgggc cggtttgccg gtggtgctcg tctcgagcgg ctcttcgtcg cacgccgatc
      421 tgcaggcgac cggttgcttg acgatcggct cgctcgagga gttgcgtgcc atcctgccgt
      481 gagcgactac tgcaggcgaa ccgaccggat catcagctcc cacagctcga gcaactcggc
      541 ggagcgttcg ctcgtgcctc cggtgaactc gaacagaaag ccgtgtccgg cgggtgcgag
      601 gaacagccag ttgtcggcct cgtcgcgcaa gacccgtgcc gcgcggtgcc gcccgggctt
      661 gtccgattcg gtgagcgcgg cgagcgattc gcgctcggtc tcgacctcct ccgctgcgag
      721 ccgcaccagg ctcccgacct cgtcgccctc ctcgctgagg aagcgaatcg gatagccgtc
      781 gaccgagcgc agcgacgcgg ccgggctgat cccgagcgac acctcggcac gcaagccggg
      841 cgtggacagg tatccctcga cccacggctg cttctggcgg tcggcgcgcg tcaggcgttc
      901 gaacttgatg ccgaagcgct cggccggatc gatcgggggc ggggccggtg cgctgcgctc
      961 gtcgcgccag gtggccggaa tccagtagag actggtcgac cgcgtcccgg ccaggaccgg
     1021 caggccgatc tcgtagccgt gatcggtgaa cttcaaccga aagagcagcc actcgccggc
     1081 gaagccgggc tccgtccaac gctccgcggc gcaagccgac gccccgctgc gctccgccgc
     1141 caggtcgaag ctctccatag cctgacgctc gaccggacgt ccgcgcaacg ggagcggtgc
     1201 cagcttgccg tcgtcccagc cggcgagtgt gtcgacccgt gcgacgtagc cgaagcgatt
     1261 gccatcgccg accgccacga tctggccgtc gctgtcccgc gttctgccgc gaaaccacag
     1321 ccgcccgcca tcgagctgac gcggtggcgg aacgtccccg tccatccgcc gggcgcggaa
     1381 gccgggtggg agtccgatgg tgaagtcacg cgcgcgaacg acctgccacg gggcctcctc
     1441 ctcgctcgac tcccccggcg tttcgtgcgt cagcggcaag ctcagcggga cgcccggcgg
     1501 aaccggccgg ccggccttcc acgcggcctc ccgcacttcg acccgtcgca atacggcgaa
     1561 cagctcgtcc gtcaggctcg ccaccgcctc ctggcccagc ccgccatcgc cgggcagcag
     1621 caacgcaccg agccgcggct ggtccggccc cagcacccgt tcgatccaca acacgggccg
     1681 gcggccggcg gccgtctcga gcgagccatc ccaggcgtct tcagcggggt cgtcctcgga
     1741 cagtcgcgcg agcccggcgc cggggaccgg cggcacgtag gcgtcgccgg gctcccccgc
     1801 gggcaacgcg tagagcgaca gcaccgtgtc gcccggccac cgccggagct cgagcaccgc
     1861 gcgcgcctcg ccgtcgggcg gcagggaggc gaaccagcgc gagtccgtat cgaccgcgag
     1921 cggcccctcg ccgaaacggt actgcgtgcc gaggacgaac accgcctgga gcacgagccg
     1981 cggatcggtt cccggctcga gcagctccgg acgctcggcg atggcgtcga ccgtcgtgcg
     2041 gggaaggtcg gcgagcagcg tcgctgccgg gcgcggttcg ccgctgcccg ggtcggccgg
     2101 tccctcggcc tgaaggagct ggctgcatac cagagccgtc gagacgacgg caacggccag
     2161 cctcagttct tttcgcggcg cacgcgctcg agctcgcgct tcatctcctc gagccgccgc
     2221 tcgagccgtt cgatctgcag ctcgagcgcg tgggccgtat ggccctccgg ccccgagtcg
     2281 gctggcggcg gcgcaggagc ctccgtcacg accggcggca cgacctcgag ttgtgcgggc
     2341 ttccttgcgc ggacgacttc gacgatgaac ggtgctcgac ggttccacga cgagaggtta
     2401 cctcgaagat cccgctccgt cctaatctcg gtttcgccga cccgcacgac gacgtcgccg
     2461 acttcgaccc ccgcctcggc ggcggccccg ccggcatcga cgcgcgtgac gagcacgccc
     2521 gccccggccg gcgcaccgaa atgctgacgc aacgccggcg tgatctcctc gacctcgaga
     2581 ccgacccgac ccacggtcca caccgtcggt tgaggcggca gcagcggtcg cgtcggcagc
     2641 gggacgaccc acggctcggg gaccgtctcg cgccgcgacc gtcgatcgcc gagcgtcgcc
     2701 gtccactcga tcgcctgccc ggcacgaaga cccttgagcc gcaacgcgtc cccgggcacg
     2761 agctcgcgta gcacgcggcc gaggtcgccc tcgttgccga ccgcggttcc gttggcctcg
     2821 aggaacacgt cgcccgagcg taggccggcg aggtccgccg gcccgccgcc gacgaccgcg
     2881 ttgacctgcg cgcccccgtc gaccgcgtcc gagagccaga cgccgagcca ggcacgtgcc
     2941 tccccgcgcg actcttcccg atcccgctcg cgctcacccg acgaaacggg gagccacatc
     3001 gcgcatccca cgatgaaggc gacggtcgcg agcttgatcc gcattccggc gtttcctctc
     3061 gatcgggtgt cttccctttg atggtacgcc catccgcggc ggaaagacga gtcccttcac
     3121 tcggcggcgt ccaggatcgc ctcgccgatc agcgcccatt gccagcggcg caagccgggg
     3181 gtgtcctgcg ggttttcccc gcggtcggcg cgttcgagcg cggcctcgac gaccgcgcgc
     3241 gggccgacga gcgacgggtc gagcccgagc tcgaccgcca ccccattccg ccgctcgacc
     3301 aaccatttga gccgcgccga cttgcgggcg acccgtcgcc cgacgtccag cgcggtggga
     3361 gccggcagct cctcctcgcc gagcgacagc gcccgctcga ccgcctcgct ccagcgcgcc
     3421 gccgcaccgc tcgcgcccgc gctctcccgc cgatcgagca gctgcgcgat ccgctcgcgc
     3481 gagcggggcc gctcgcgcgc gatgcgcagc agcagctcgt tgtgcgcgat gcggaacggt
     3541 ggtcgatcgg cggcgcgcgc cagccgttcg cgcagcacgt gcaactcgcg cagcacggcc
     3601 aaaccgcgtc gatcgagcgt cgcgctgccg cgcaccgccc ggtacgcttc gcggtcgtcg
     3661 ggttcgctga agcgaatccg ctcgagcacg ccgaactcct cttcggccca ggtgatgcga
     3721 ccgagtgcct gcaaccggga ttcgagccgg gaggacagct cgacgagatg gcgggtgtcg
     3781 agcaccgcgt agcggatcat cgcggccggc agcggccgac gcgaccagtc cgcccgctgg
     3841 aatcgcttgt ccagctggac gccgaggtat cggtccaaca gtgcggccag gccgaactgc
     3901 cgttcgccgc acagccgtgc cgccaccatc gtgtcgaaca gcccggcgac gcgcagaccg
     3961 agatcgcgat cgatcacccg caggtcgtag tccgcgccgt gcagcacctt gcggatccgt
     4021 cgctcggcga gcaatggccc caacggctcg aggtcgacgc ccgccagcgg gtcgatcagc
     4081 aggtcgcggc cagcgctcga cagctgaacg agacagatct tctccggata gtggtggagc
     4141 gaatccgatt ccgaatcgag cgcgatcgga ccgctcccga tccgcccgat ctcgcgcgcc
     4201 agatcctccg ttcgcgtgca ccagacgtac gaaccggcca tggcgtgcga ggctagccgc
     4261 tcggcagaat cggcgtcaag gccggggttt cgcccgaaca caccacgcgc tatgctgccg
     4321 gcgtggtccc gggtcgcccg agcaccccgg gcgaggaggt tcgacgatga ccaggattgc
     4381 catgtgcatg atgctgccgc tcttgatccc gtctgtcgtc gttgccggag aagtggccgg
     4441 cgtcgcgatg cccgacgcgg tcaccgtcga cggcgtcgaa ctgacgctga acgggattgg
     4501 cctgcgcgag aagctgtggg tcgacgtcta tgtggccggg ttgtacctcg agcgagcctc
     4561 cgacgatgcc cgccagatca tcgactccga gcagaccaag cagttgcaga tgcaattcgt
     4621 ctacaaggtc ggcaagcaga agctcaccgg tgcgtggacc gcgggcctcg aggccaacca
     4681 accgaaccgt ttcgtcgagc tcaccgagca gttgaaccgg ttgaacgcga tgatggacga
     4741 cgtggccgac ggcgacgtga tgcggttcac cacgcgtccg ggacaggggt tgcgtgtcga
     4801 ggtgaagggt gtcgacaagg gtctgatcga gggtgacgag ttcgcccgcg cgttctggtc
     4861 gatcttcctc ggcgacaagc cgccgacgga gaagctgaag agtgggctgc tcggcgggtc
     4921 gtagcctcgc tctgcggcga ccgagcgcgg gcgcggcgat cgcgcggcac ggcaggctgc
     4981 cggcggtcca ccggtgacgg cggtgctcgg cgtccactcg gagatcggtc gcctgcgcgc
     5041 cgcgctggtc cacgagccgg gccaggagat cgatcggatg gtgccggcga tg
//
LOCUS       DASPGN010000517         3644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1382601, whole genome shotgun sequence.
ACCESSION   DASPGN010000517 DASPGN010000000
VERSION     DASPGN010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1382601"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VD788_15050"
     CDS             <1..560
                     /locus_tag="VD788_15050"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXV77629.1"
                     /translation="PGCSGLEVAASLPAPRPQIVFCTAYDQHAVEAFELHAVDYLLKP
                     VSRARLAKAVERVVAGGPGAAVDSAIDRVVPAPTRFLARRGNRYQVIPREEVLCFVSD
                     GGLTTVLTGGGTLWMQPSLAELEQRLDPDGFFRISRAAIVNLDAIREVRPAGGGQAEL
                     HLRDGRTLEVSRRRYRPLMVKLGEV"
     gene            complement(567..1451)
                     /locus_tag="VD788_15055"
     CDS             complement(567..1451)
                     /locus_tag="VD788_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXV77630.1"
                     /translation="MRRSAWVVPLLVIAACGPRPDEAPGQTAGTASAHGVVESFDGVP
                     VAYSSYGTGEGTLVFVHGLSCDRGYWDAQTEAFDDVYHVVTVDLGGHGESGDDRAEWT
                     IRSLARDVEAVIERLDLGPVVIVGHALGGWVALDVARSIPERVVGVIVVEALQSVSAD
                     RPADPIGQPARWQEDFGGYCRELAGTMFHDGADPALRERITADLCGAPPDIATALVRA
                     ITDHDAAAALAATPVPVRALNGDLSPTDVEGNRRHHPDFDATVIEGTGHFPMLERPAD
                     FNRMLERIIEELTAGATT"
     gene            complement(1486..2442)
                     /locus_tag="VD788_15060"
     CDS             complement(1486..2442)
                     /locus_tag="VD788_15060"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXV77631.1"
                     /translation="MRRSWITVALAATLLAGASPVLADTRVERELALGSGGRFILDSD
                     TGSVEVAGRSASGVRVVITSSRDDFESRFDLSFDERPGEAEVKVERKDRGIGGWFRSD
                     GDVRFTIEVPADAEVEIDTAGGAIEARDIGAAARLDTSGGSISAVRIRGTLEADTSGG
                     SITIEEVDHDVSADTSGGSIRIASVRGAVHADTSGGGITIHGATGDVVADTSGGSIEV
                     EEAGGQVRADSSGGPIRVSFAPGNARGGSISTSGGGITVEIDPGIGLEIDAASSGGTV
                     DFDMPLLVQGRVGRESVRGTLGSGGEALRLRTSGGGIRILSR"
     gene            2539..>3644
                     /locus_tag="VD788_15065"
     CDS             2539..>3644
                     /locus_tag="VD788_15065"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXV77632.1"
                     /translation="MATEHDDGGGGGRGPSLGRLFVDDLRAARLGRDHWQELKDLYHF
                     YLDEDDRARLAGMGRLRRAFMLLGWLFKSLLRKLSPGRRLMLLIAMVFTVMGDTSFVV
                     VGWGFRADFRPWAVLFVTIVLMLELKDKLVARDEIQIAREVQLALLPRENPSLPGWSV
                     WCYSRPANDVGGDLVDYIELDGFRHGVLLGDVAGKGLGAALLSAKLQATLRALVPEAA
                     SLDDLGRRTNAIFNRDGIANRFATLFYAELEYNSGQLRYLNAGHNPAYVVRPGGVLKL
                     TASSWPIGMLPEASYEECSIDLAPGDLLLAYSDGLTEARNASGEEFGAPRVERLLGEM
                     RDLSPEQVGGRILREVDRFLGEARPSDDLSLVVLV"
BASE COUNT          530 a         1279 c         1279 g          556 t
ORIGIN      
        1 tgccgggttg cagcgggctc gaggtcgccg cctcgctgcc cgcgccgcgc ccgcagatcg
       61 tcttttgcac ggcctacgat cagcacgcgg tcgaggcgtt cgagttgcac gcggtcgact
      121 acctgctgaa gccggtcagc cgcgcacgtc tggccaaggc cgtcgagcgt gtcgtcgccg
      181 gcgggccggg cgcggcggtc gactcggcga tcgaccgggt cgtccccgca ccgacgaggt
      241 ttctcgcccg gcgcggcaat cggtaccagg tgatcccgcg cgaggaggtg ctgtgtttcg
      301 tctccgacgg cggcctgacg accgtcctca cgggcggcgg cacgctgtgg atgcagccga
      361 gtctggccga actggagcag cggctcgacc ccgacggttt ctttcgcatc tcgcgcgcgg
      421 cgatcgtcaa cctcgacgcg atccgtgagg tgcgcccggc gggcggcggg caggccgagc
      481 tgcaccttcg cgatgggcgg acgctcgagg tcagccggcg ccgctaccgg ccgctgatgg
      541 tcaagctcgg cgaggtttag gcgcgctcag gtcgttgcac cggcggtcag ctcctcgatg
      601 atccgctcga gcatgcggtt gaagtctgcc gggcgttcga gcatcggaaa gtgtccggtg
      661 ccttcgatga cggtcgcgtc gaagtcggga tggtgacgcc ggttgccctc gacgtcggtc
      721 ggcgacaagt cgccgttgag cgcgcgcacg gggacgggtg tcgcggccaa cgccgcggcg
      781 gcgtcgtggt ctgtgatcgc ccggaccaac gcggtggcga tgtcgggggg cgcgccgcac
      841 aggtcggcgg tgatccgctc gcgcagcgcg ggatcggctc cgtcgtggaa catcgtgccg
      901 gccagctccc ggcagtagcc gccgaagtcc tcctgccagc gcgccggttg gccgatagga
      961 tccgccgggc ggtcggccga gaccgactgc aacgcctcga cgacgatcac cccgacgacc
     1021 cgctccggta tcgagcgggc gacatcgagc gcgacccagc cgccgagggc gtgaccgacg
     1081 atcacgaccg gaccgagatc cagccgctcg atcaccgctt cgacgtcgcg cgcgagcgac
     1141 cggatcgtcc actccgcgcg atcgtctccc gactcgccgt gaccgccgag gtcgaccgtc
     1201 accacatggt agacgtcgtc gaatgcctcg gtctgcgcgt cccagtaacc gcgatcgcag
     1261 gacagaccgt gaacgaagac gagcgtgccc tcgccggtgc cgtacgagga gtaggcgacg
     1321 gggacaccgt cgaacgattc gacgacgccg tgcgcggagg ccgtgccggc ggtctggccg
     1381 ggggcctcgt ccggacgtgg cccgcaggcg gcgatgacga gcaacggcac gacccacgcc
     1441 gacctgcgca tgggccaagt ctatcagcac cggccgggcg cggagctagc gcgagaggat
     1501 gcggatcccg cccccggaag tacgcaggcg cagggcctcg cctccgctgc cgagcgttcc
     1561 gcgaaccgac tctcggccga cccgaccctg caccaggagc ggcatgtcga agtcgaccgt
     1621 gccgcccgac gaggcggcgt cgatctcgag accgatcccc ggatcgatct cgaccgtgat
     1681 cccgccgccc gaggtcgaaa tgctgccgcc gcgcgcgttg cctggcgcga acgagacgcg
     1741 gatcggcccg cccgaactgt cggcgcgcac ctggccaccg gcctcctcga cctcgatcga
     1801 accacccgag gtgtcggcga cgacgtcgcc ggtcgccccg tggatcgtga tgccgccccc
     1861 cgacgtgtcg gcgtggaccg cgccccgcac cgacgcgatg cggatcgagc cgcccgaggt
     1921 atcggcgctg acgtcgtgat cgacttcctc gatcgtgatc gagccacccg aggtatcggc
     1981 ctcgagcgtg ccgcggatac gcacggcgct gatcgaacca cccgacgtgt cgagccgtgc
     2041 cgccgcgccg atatcgcgcg cttcgatcgc tccgcccgcg gtgtcgatct cgacctcggc
     2101 gtccgcgggt acctcgatcg tgaagcggac gtcgccgtcc gaacggaacc agccgccgat
     2161 gccgcgatct ttgcgctcga ccttgacctc cgcctcgccc ggccgctcgt cgaacgacag
     2221 gtcgaacctc gactcgaaat cgtcccgcga cgaggtgatc acgacgcgga ccccggacgc
     2281 cgagcgaccc gcgacctcga cgctccccgt atcggagtcg aggatgaacc tcccgcccga
     2341 tccgagggcg agttcgcgct cgacgcgcgt gtcggccagc accggggaag cgccggccag
     2401 cagcgtggcc gccagtgcca ccgtaatcca cgatcttcgc atcctgtacc tcctcccagg
     2461 aaccggacgg acggcgcccg gcccctcgcc caattaagga cgcccgagcc cggccgaagg
     2521 ttgcaataat gggatcatat ggccacggag cacgacgacg ggggcggggg cgggcgcggc
     2581 ccgagcctgg gccggctgtt cgtcgacgac ctgcgggcgg cgcgcctggg acgcgaccat
     2641 tggcaggagc tgaaggacct ctaccacttc tacctcgacg aggacgatcg cgctcgcctg
     2701 gccggtatgg gtcgcctccg ccgcgcgttc atgctgctcg gctggttgtt caagagtctg
     2761 ctgcggaagc tgtcacccgg ccggcggctg atgctgctga tcgcgatggt cttcaccgtg
     2821 atgggcgaca cgtcgttcgt cgtcgtcggt tggggctttc gcgcggattt cagaccgtgg
     2881 gccgtgttgt tcgtgacgat cgtgctgatg ctcgagctga aggacaagct ggtggcgcgg
     2941 gacgagatcc agatcgcgcg cgaggtgcag ctcgcgctgc tgccgcgcga gaacccgtcg
     3001 ctgcccggat ggtccgtctg gtgctactcg cgaccggcga acgacgtcgg cggcgatctg
     3061 gtcgactaca tcgagctcga cggctttcgc cacggcgtgc tgctgggcga cgtggccggc
     3121 aaggggctcg gcgcggcgct gttgtcggcc aagctccagg cgacgttgcg cgcgctcgtg
     3181 cccgaggcgg cctccctcga cgatctgggg cgccgcacca atgcgatctt caaccgcgac
     3241 gggatcgcca accggttcgc cacgctgttc tacgccgagc tggaatacaa ttcgggacag
     3301 ttgcgctacc tcaacgccgg acacaacccg gcctacgtcg tccggccggg cggcgtgctc
     3361 aagctgaccg cctcgtcctg gccgatcggc atgctgcccg aagcgtccta cgaggagtgc
     3421 tcgatcgatc tcgcgccggg cgacctgctg ctggcctact ccgacgggct gaccgaggcc
     3481 cgaaacgcga gcggtgagga gttcggagca ccgcgggtcg agcgactgct gggcgagatg
     3541 cgcgacttgt cgcccgagca ggtcggcggg cgcatcctgc gcgaggtcga ccgctttttg
     3601 ggtgaagcgc gcccatccga cgacctgtcg ctggtggtgt tggt
//
LOCUS       DASPGN010000518         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_96450, whole genome shotgun sequence.
ACCESSION   DASPGN010000518 DASPGN010000000
VERSION     DASPGN010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96450"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VD788_15070"
     CDS             <1..570
                     /locus_tag="VD788_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77633.1"
                     /translation="DGQGTNGSANGNNTLALPFNQQTNLGNAFQLITDIGGTTIVEQI
                     AQFDRTSDGFVGYTGTAGDAFTLVSGDAYLVQLKLGVPGVNYIVVGSHNPGKTILFDG
                     QGTNGSANGNNFYAAPYHSTASTAGDLIMELGGTTAIEQIAFLDRTTDGFVGYTGTSG
                     DAFDLDPGRGYIVQLKLGVPNVQFVPSHF"
     gene            599..1801
                     /locus_tag="VD788_15075"
     CDS             599..1801
                     /locus_tag="VD788_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77634.1"
                     /translation="MKPLTKIAIATAAVALLAATMQPAEAACSVPKLINTGNTYLVSN
                     PDWGAASTGGDGSCTTDYGCYESESGPPITDGIGGTFWAWAEGAPEGGDSTYFVGTDN
                     GTWEASQWTKQISLPYAGGLYHFQAWITLPGGVPYISGFVPNWGASFEIDGCTTDITP
                     TIDNDECTCMLLTDEWNGQGYFLAFSAVNIGNGNFNFDRFEHVNMAPIPTPSVAMSER
                     DDATGDVLFSVNVPALPAAGDYRQDGCHCDLGFRVYAQLTGREGAPPAGRRSCSPTDY
                     QNDAAFDGTAASIRQVCQGRGLNWIPAQAAAGGPQAPTPFGQTATVRVDCDPAVQSDV
                     YLSTEIVGDSVGVMSGRHVSGNSFRVECGANVVDDPTRPDRDQGQSDEAPRGRDENRG
                     RGNATGRR"
     gene            complement(1926..>3392)
                     /locus_tag="VD788_15080"
     CDS             complement(1926..>3392)
                     /locus_tag="VD788_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77635.1"
                     /translation="RVTGQSTPATARDDRGDLGWVAAVFLLAVAARLFYLGRLETPAF
                     DPWRHLALVRNIRDGAGFTLFDGQPYLWYGPAWYYLLAALPRWFRFEWFTALLSAACA
                     PLTYLWLRRTAGRRGGVVAPFVASLMMALSGPVVAYTCHYGPEAPALLSTLTGLVLCS
                     LAPARGWAWSLAGGLAYGLALTLRLNFIVSGPLFLPWMTKRRRDAGLIVLGAAIPLAA
                     NWWRNHAILERYAWVFTWDGLATRTADFDFVSTLVIQRHPAVGEALRRLHLQIVPHPE
                     WLFDGDGVAWGPLVFLVAGLAGALAARSGFLLLSALGGFAYFALFDGSMSANFFRIHL
                     PIFPALFAGLALASVGRTARRRAVALALVGFALLGGSVLFEPPGMIGLPAVTPPAGLL
                     SRSSYAVNSGFFQPEAIAWAHPELDLIGLPLEPDDLDDLLADFPDHRAILWHETGAVQ
                     PELRARLESDGRFESLGRARNDDGFLYEVFGVAGSAAP"
BASE COUNT          614 a         1117 c         1188 g          473 t
ORIGIN      
        1 gacggtcagg gcacgaacgg ctcggccaac ggcaacaaca ccttggcgct tccgttcaac
       61 cagcagacaa acctgggcaa cgcgttccaa ctgatcaccg acatcggcgg tacgacgatc
      121 gtcgagcaga tcgcgcagtt cgatcgcacg agcgatggtt tcgtcgggta cacgggcacg
      181 gcgggcgacg ccttcacgct cgtttcgggc gacgcgtatc tcgtccagct gaagctcggc
      241 gttccgggcg tcaactacat cgtcgtcggc tcgcacaacc ccggcaagac gatcttgttc
      301 gacggtcagg gtacgaacgg ctcggccaac ggcaacaact tctacgccgc accgtatcat
      361 tcgaccgcgt ccaccgcggg cgatttgatc atggagctcg gcggcacgac cgcgatcgag
      421 cagatcgcgt tcctcgaccg aacgaccgac ggtttcgtcg ggtacaccgg tacgtccggt
      481 gacgccttcg acctcgaccc cggcaggggg tacatcgtgc agctgaagct cggtgtcccc
      541 aatgttcagt tcgtgccttc gcatttctaa tcaatcgacg caggaggagt acggaattat
      601 gaagccattg acgaagatcg cgatcgccac tgcggccgtg gcgctcttgg cggccacgat
      661 gcagccggct gaggcggcgt gcagcgtgcc caagttgatc aacacgggta acacctacct
      721 ggtgtcgaac ccggactggg gcgccgcatc gaccggtggt gacggttcat gcaccacgga
      781 ctacggatgc tacgagagcg agagcggacc gccgatcacc gacggcatcg gtggcacgtt
      841 ctgggcttgg gccgaaggcg ctccggaagg cggcgactcg acctacttcg tgggcaccga
      901 caacgggacc tgggaagcca gtcagtggac gaagcagatc agcctgccct acgctggcgg
      961 tctttaccac ttccaggcgt ggatcactct gccgggtggc gtgccgtaca tctcgggctt
     1021 cgtgccgaac tggggcgcgt cgttcgagat cgacggttgc accaccgaca tcacgccgac
     1081 gatcgacaac gacgagtgca cctgcatgct gctgacggac gagtggaacg gccagggtta
     1141 cttcctggcg ttctccgccg tcaacatcgg caacggcaac ttcaatttcg atcgcttcga
     1201 gcacgtcaac atggcgccga tcccgacgcc ttcggtcgcg atgtccgagc gcgacgacgc
     1261 caccggtgac gtgctgttct cggtcaacgt gccggcgctc ccggccgcag gcgactatcg
     1321 ccaggacggc tgccactgcg atctcggctt ccgcgtgtac gctcagctca ccggacgcga
     1381 gggtgcgccg ccggccggcc gtcgcagctg ctcgccgacg gactaccaga acgatgcggc
     1441 gttcgacgga accgcggcgt cgatccgcca ggtttgccag ggtcgcggtc tcaactggat
     1501 tcccgcgcag gctgcggctg gcgggccgca ggccccgacc ccgttcggcc agacggcgac
     1561 cgtccgcgtc gattgtgatc cggcggtgca gagcgacgtt tacctgtcga ccgaaatcgt
     1621 cggcgattcg gtgggcgtga tgagcggaag gcatgtgtcg ggtaactcgt tccgcgtcga
     1681 gtgcggtgcc aacgtcgtcg atgaccccac tcggccggat cgcgaccagg gccaatcgga
     1741 cgaggcgcct cgcgggcgtg acgagaatcg cggccgcggg aacgcgaccg gtcgtcgttg
     1801 atcgcgttcg cttgacggct tagtttcttc ttctgcggaa gaggcagggc cgcgggacca
     1861 gcgaggtccc gcggcccttt tcgttcgtac gcccgagcga cacgtcgcgc cgcgcgcgac
     1921 aggactcacg gcgcagcgga acccgccacg ccgaacacct cgtacaggaa cccgtcgtcg
     1981 ttgcgtgcgc ggccgagcga ctcgaagcgg ccgtcgctct cgagacgcgc gcgtagctcg
     2041 ggctggaccg cgccggtctc gtgccacagg atcgcgcgat ggtcgggaaa gtccgccagg
     2101 aggtcatcga ggtcgtccgg ctcgagcggc agcccgatca ggtcgagctc ggggtgagcc
     2161 caggcgatcg cctccggctg gaagaagccg ctgttgacgg cgtagctcga tcgggagagc
     2221 aagccggcgg gaggcgtgac cgcgggcagc ccgatcatgc cgggtggctc gaacaggacc
     2281 gacccgccga gcagcgcgaa gccgacgagc gcgagagcga cggctcgccg ccgcgcggtc
     2341 cgccccaccg aggcgagggc gagcccggcg aacagcgccg gaaagatcgg caggtggatg
     2401 cggaagaagt tggccgacat cgagccgtcg aacagcgcga aataggcgaa tccaccgagc
     2461 gccgacagca gcaggaaccc gctgcgggcg gcgagagcgc cggccaagcc cgcgacgagg
     2521 aacaccagcg ggccccaggc gacgccgtca ccgtcgaaca gccattcggg atgggggacg
     2581 atctggagat ggagacgccg cagcgcctcg ccgacggccg ggtgccgctg gatcacgagg
     2641 gtcgagacga agtcgaagtc ggccgtgcgt gtcgccaggc cgtcccaggt gaagacccac
     2701 gcgtagcgtt cgaggatcgc gtggttccgc caccagttcg cggcgagcgg gatggcggcg
     2761 ccgagcacga tcagcccggc gtcgcgccgg cgcttggtca tccacggcag gaacagcgga
     2821 ccgctgacga tgaaattgag ccgcagcgtc agcgccagcc cgtacgccag gccgccggcc
     2881 agcgaccagg cccacccacg tgcaggggcg agcgagcaca ggacgagccc ggtgagcgtc
     2941 gacagcagcg cgggtgcctc cggaccgtag tggcacgtat aggccaccac ggggcccgac
     3001 agcgccatca tcagcgaggc gacgaacggg gcgacgacgc cgccacgacg ccctgcggta
     3061 cggcggagcc acaggtaggt caacggggca caggcggccg acagcagcgc ggtgaaccac
     3121 tcgaagcgga accagcgtgg cagagcggcc agcaggtagt accacgcggg gccgtaccag
     3181 aggtacggct gaccgtcgaa caacgtgaag ccggcgccgt cgcggatgtt ccggacgagg
     3241 gcgaggtgcc gccacgggtc gaaggcgggc gtttcgagcc ggccgaggta gaacagtcgc
     3301 gcggcgaccg cgagcagaaa gaccgcggcg acccagccga gatcgccgcg gtcgtcgcgc
     3361 gcggtcgccg gggtcgactg gccggtcacg cg
//
LOCUS       DASPGN010000519         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_96835, whole genome shotgun sequence.
ACCESSION   DASPGN010000519 DASPGN010000000
VERSION     DASPGN010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96835"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..494)
                     /locus_tag="VD788_15085"
     CDS             complement(33..494)
                     /locus_tag="VD788_15085"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXV77636.1"
                     /translation="MRWPRYRFRRPVEPTVPVFDTDARRSDAPRPPGRPDPESLRAEK
                     LKRRVRILAFHVVFVSGLLAALVGQGGYLELVRKRAERDAAERRLVEQQRRVDALAER
                     IRRLEHDPMARERIAREQLGLARPGEVQFLLPREPVLPLGEPQTPGPDVAD"
     gene            complement(491..1831)
                     /gene="eno"
                     /locus_tag="VD788_15090"
     CDS             complement(491..1831)
                     /gene="eno"
                     /locus_tag="VD788_15090"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXV77637.1"
                     /translation="MRAERNGQIHEIVGRQILDSRGNPTVEVDVILVGGATGRAMVPS
                     GASTGTREALELRDKQSKRYGGKAVTKAVDHVNKVIGPALTGIEVVNQVFIDHAMIEM
                     DGTENKSKVGANAMLGVSIAAARAAAEWLGLPFYRYIGGCDTTDLPVPMMNVLNGGAH
                     ADNNIDIQEFMIVPVGAASYSEALRCGVECYHALKEVLKKRGLGTAVGDEGGFAPNLR
                     SNEEALEVVCEGVVKAGAKPGKDVVLALDVAASELYSKGQYTLAAERRSTMSAADLVA
                     MYAAWSDKYPIASIEDGMAEGDWKGWKLLTETLGGRLQIVGDDLFVTNSRILAKGIRE
                     GVANSILIKLNQIGTVTETLETIRLALRAGYSCVVSHRSGETEDTTISDLAVALNLGQ
                     IKTGAPCRGERNAKYNRLLRIEEELGDDARYPGRAVFPRLARFEARARGAAGPR"
     gene            1994..2347
                     /gene="cutA"
                     /locus_tag="VD788_15095"
     CDS             1994..2347
                     /gene="cutA"
                     /locus_tag="VD788_15095"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HXV77638.1"
                     /translation="MNERSDDFLLVLTTAGSAEQARAIARGLVERRLVACANVVPGAL
                     SIYRWQGRVEEQAESLVVLKTAKRLFPELRRVIRELHSYELPEILALPIADGDPDYLD
                     WLGGALADENTRAGS"
     gene            complement(2356..>3322)
                     /locus_tag="VD788_15100"
     CDS             complement(2356..>3322)
                     /locus_tag="VD788_15100"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HXV77639.1"
                     /translation="LAELEGGADALAFSSGMAAIASVAQLLEAGDHVLATENAYGGTR
                     RLFDGLMTRFGLEFDWVDTSSTERIAAAFRPTTRMVFIETPTNPTLTLTDIAAVVEII
                     RQRRARLVVDNTFMTPCLQRPLALGADVVVHSTTKYLNGHSDSIGGAAIARDPADAER
                     LGWIQRSVGAVLSPLDSFLVLRGIKTLSLRMARHQSSAVEIARWLERQPTVARVHYPG
                     LDSHPQRALAARQMGGAGGVVSVDLGSRARAERFVAGLELWQLAESLGGVESLVSHPA
                     TMTHAWLDRAERERIGIGDGLLRLSVGVEDPADLLEDLERALTGV"
BASE COUNT          483 a         1210 c         1094 g          535 t
ORIGIN      
        1 gacggcgccg atcaaccccg cgtgggccgg gctcagtcgg cgacgtccgg gcccggcgtc
       61 tgcggctcgc cgagcgggag caccggctcg cgcggcagga gaaactggac ctcccccggc
      121 cgagccaggc cgagctgctc gcgcgcgatc cgctcgcgcg ccatcgggtc gtgctccagg
      181 cgtcggatgc gctcggccag cgcgtcgacc cgtcgctgct gctcgacgag cctgcgctcg
      241 gcggcatctc gctcggctcg tttgcgcacc agctcgaggt agcccccctg cccgacgagc
      301 gccgcgagca gaccgctgac gaagacgacg tggaacgcga ggatccgcac acgacgcttg
      361 agcttctcgg cccgaagact ctcggggtcc gggcgtcccg gcgggcgcgg ggcgtccgag
      421 cgacgtgcgt cggtgtcgaa gacggggacc gtcggctcga ccggccggcg gaagcggtag
      481 cgcggccacc tcatctcgga ccggccgccc cgcgcgccct cgcctcgaac cgggcgaggc
      541 gagggaagac cgcgcgtccc gggtagcgcg cgtcgtcccc gagctcttct tcgatgcgta
      601 acagccggtt gtacttcgcg ttgcgctcgc cgcgacacgg tgcgccggtc ttgatctgcc
      661 ccaggttcaa tgcgacggcc aggtcggaga tcgtcgtgtc ttccgtttct cccgagcgat
      721 gcgagacgac gcagctgtag cccgcacgca gcgcgagtcg gatcgtctcg agcgtctcgg
      781 tcaccgtgcc gatctgattc agcttgatca ggatgctgtt ggcgacgccc tcgcggatcc
      841 ccttggccag gatccgcgag ttggtgacga acaggtcgtc cccgacgatc tgcagccggc
      901 cgccgagcgt ctcggtcagc agcttccagc ccttccagtc accctcggcc atcccgtcct
      961 cgatcgaggc gatcgggtac ttgtcggacc acgccgcgta catcgccacc agatcggccg
     1021 cgctcatcgt cgaccgtctc tccgccgcca gcgtgtactg ccccttcgaa tagagctcgg
     1081 aggcggcgac gtcaagcgcc aataccacgt ccttgcccgg cttggccccg gctttcacca
     1141 cgccctcgca gacgacctcg agtgcctcct cgttgctacg caggttgggc gcgaagccgc
     1201 cctcgtcgcc gaccgccgta cccaggccgc gcttcttcaa cacctccttc agcgcgtggt
     1261 agcactccac gccgcagcgc aacgcctcgg agtaggaggc cgcaccgacc ggcacgatca
     1321 tgaactcttg gatatcgatg ttgttgtcgg cgtgcgcacc gccgttcaac acgttcatca
     1381 tcggcaccgg cagatcggtc gtgtcgcagc cgccgatgta gcgatagaac ggcaggccga
     1441 gccactcggc ggccgcgcgc gcggcggcga tcgacacgcc gagcatcgcg ttggcgccga
     1501 ccttcgactt gttctcggtg ccgtccatct cgatcatcgc gtggtcgatg aacacttggt
     1561 tcacgacctc gatcccggtc aacgccggac cgatgacctt gttgacgtga tcgaccgcct
     1621 tggtcacggc cttgccgccg tagcgtttcg actgcttgtc gcggagctcg agcgcttccc
     1681 gcgtgccggt cgaggcgccc gacgggacca tcgcccgccc cgtcgcaccg ccgacgagga
     1741 tcacgtcgac ttcgaccgtc ggattgccgc gggaatcgag aatctgccgg ccgacgatct
     1801 cgtggatctg cccgttccgc tccgctcgca tccgcactac tcccgttccg ttgtcccgtc
     1861 gagggacgtg ccgggcgact atagcgcaac ccacccgccg gtctcaaggg caaccctatc
     1921 tgttttcagc aagatacgac tgccgttcgt gctatgctgt cctgccccgc aagcgcacgg
     1981 gcggagcgcc ccgatgaacg aacgatccga cgacttcctg ctggtgctga cgacggcggg
     2041 atcggccgag caggcgcgcg cgatcgcgcg cggcttggtc gagcgccggc tcgtcgcctg
     2101 cgccaacgtc gttcccggtg cgctctcgat ctatcgctgg cagggccggg tcgaagagca
     2161 agccgagtcg ctcgtcgtcc tcaagacggc gaagcgcttg ttccccgagc tccgccgcgt
     2221 gatccgcgag ctgcactcct acgaactgcc cgagattctc gccctgccga tcgccgacgg
     2281 cgatccggac tatctcgact ggctgggcgg cgcgctggcg gacgagaata cgcgggccgg
     2341 ctcgtaaggc gcggcctaca ccccggtgag cgcccgctcg aggtcctcga gcaagtccgc
     2401 cgggtcctcg acgccgaccg acaaccggag cagcccatcg ccgatgccga tgcgctcccg
     2461 ctccgcgcgg tcgagccacg cgtgggtcat cgtcgccggg tgggagacga gcgattcgac
     2521 gccgcccagg ctttcggcca actgccacag ctcgagcccg gcgacgaacc gttcggctcg
     2581 ggctcggctg ccgaggtcga cgctgacgac cccgcccgcg ccccccatct gcctggcggc
     2641 gagagcgcgc tgcgggtgcg agtcgagccc ggggtagtgg acccgcgcga ccgtcggctg
     2701 ccgctcgagc catcgcgcga tctcgaccgc gctcgactgg tgccgcgcca tgcgcagcga
     2761 cagcgtcttg atcccgcgca acaccaggaa gctgtcgagc ggcgacagga ccgcaccgac
     2821 ggaacgctgg atccagccga gccgttcggc gtccgcggga tcgcgggcga tcgccgcgcc
     2881 gccgatgctg tcactgtggc cgttcaagta cttggtcgtc gagtgcacca ccacgtccgc
     2941 gccgagcgcc agcggccgtt gcaggcacgg ggtcatgaag gtgttgtcga cgacgagccg
     3001 tgctcgtcgc tggcggatga tctcgaccac cgccgcgatg tcggtcagcg tcagcgtcgg
     3061 gttggtcggt gtttcgatga agaccatccg cgtcgtcgga cggaaggccg ccgcgatccg
     3121 ctcggtcgac gaggtatcta cccaatcgaa ctcgagaccg aagcgggtca tcagcccgtc
     3181 gaacaagcgg cgcgttccac cgtatgcgtt ctcggtcgcg agcacgtggt cgccggcttc
     3241 gagcagctgg gcgaccgagg cgatcgccgc catcccggag ctgaacgcca gcgcgtcggc
     3301 gccgccctcg agctccgcga gc
//
LOCUS       DASPGN010000520        17260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_97351, whole genome shotgun sequence.
ACCESSION   DASPGN010000520 DASPGN010000000
VERSION     DASPGN010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 17260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17260
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97351"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VD788_15105"
     CDS             complement(<1..361)
                     /locus_tag="VD788_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXV77640.1"
                     /translation="MSAARRLAAAVLALLALFRPALGVGADGGRLGGVLVAADGATAA
                     GFTVHLIDGVGHEVASAEVGADGVYAFDHVPAGRYALGIGNPAGQRAPVATPAIVVTP
                     GATVRRDLLLVGADAAAG"
     gene            complement(358..918)
                     /gene="pyrE"
                     /locus_tag="VD788_15110"
     CDS             complement(358..918)
                     /gene="pyrE"
                     /locus_tag="VD788_15110"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXV77641.1"
                     /translation="MKELLRKHSLIFGDFTLSSGKKSRYYFDSKKTTLLAEGAYLAAV
                     EVLELLRARAIRADAIGGLTLGADPIVCPVAALSQLDGRPLRAFIVRKEAKEHGTGRR
                     IEGSLEPGSRVVVVDDVVTTAASTLKAIEAVEAEGHTVVAVVCIVDREEGGTEKLAGW
                     PFYPIFKRSEIFSPPDGESLDDPEGQ"
     gene            1067..1591
                     /locus_tag="VD788_15115"
     CDS             1067..1591
                     /locus_tag="VD788_15115"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXV77642.1"
                     /translation="MRERTAGFKLVAALVVVGLQGLAVPVMADKPTAEVRGSVLSATD
                     QEPVAGARLHLSNPETGEFFSSAPTRDDGGFVIESVPVATYDAGVEHDGGLYVIESPV
                     SIAPGQTQPLQVAIDEQLAPSPDEQEEQRKKSGTSVLNNPATAALLVLGAAIVLGVII
                     DAATDDDDESASPF"
     gene            1689..3935
                     /locus_tag="VD788_15120"
     CDS             1689..3935
                     /locus_tag="VD788_15120"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HXV77643.1"
                     /translation="MWDLWRIVVRRRTLVFAAVALSVALTAVVTLLSTPIYQATTTIQ
                     IERRGSGVLNYKDVTSVDPSFAGYQDFYQTQYRILQSDAVLRLAARRLDLPNRPDYAG
                     RESPPIARLVGWVLSALPAPSRSDRARDGETDDPHDQATSFLRARLSVQPVRGSQLVR
                     VSFLDRDPALARDSANAVAAAYQEFSLQSRYSTTAQASEFLTKQVAELQQEAAAMERR
                     LQALSAEKEILALRDDTKDISTLALTDLNAKLVESKARLALAGARYDAVRTAPADSMA
                     EVLRSPLINNLRQQYAELERKHRQMSERFRDDWPALRQVTEELTQAAARLELETETIA
                     TRVRGVARAERDQAGAEVRLLQARVDEQKQVVQRVSLDAVEIASLQAEIKTKRRFLDE
                     LVNRQNQTEVTDRLRDPQASNIRVVDAARAPDYPVRPRKLLNLLASLVAGLVLGAGAA
                     LLSDHLDNTVKNEADVRIAAGLPVLGQIRVFESTLTPSPVGPSPTHWPDQPGLGCHVE
                     PLSVFAEAFKSLRTSLLLAAPDHPPRSILITSAEPGDGKSTVAVNLSTVLTQLGKRVL
                     LVGADLRRPSVHSMLDLPNKRGLSNLLTGTADLRDVVCESAVPRLDVIPSGPIPPTPS
                     ELLGSAGMGALIDRLLNDEGYDHVVIDSPPAAQVTDAVILSAYAAATVVTARAGATTR
                     DALALTLERLRRARAHLPGIVLNGARVGGDYRYRLYYGESAGPEHGRGPERLVPRRRA
                     SRKRATGV"
     gene            3932..5383
                     /locus_tag="VD788_15125"
     CDS             3932..5383
                     /locus_tag="VD788_15125"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXV77644.1"
                     /translation="MTTGGGPRSDAATAIASFVLFALLLASPLPFGSVQPPYVLGIEL
                     GAALAGGLAAWIVWRDPGRLSGAGRVGLALAGAVLLIGGAQLLPLPRSLVAVLAGPTV
                     EARDGIALAIDAPLPRWMPLTLSPPATVDALLRWSAYLAIGLAAAVCIRSERQRLVAA
                     GLLSLAGAFQALYGSAEYLSGHQHIFWYPKRYFTDEASGTFINRNHFAAYLSICLPLA
                     AAATLDGYRRLRAVGVRWRVLVLLTEAGGSWVVLAAVATASIWIGIVLSFSRGGLAAS
                     LVGITILVGGLLRRKRGAVWLLALLAIPTAALLAMEVRAPGARFVTEADHLADFGGRL
                     EVWTATGEIVGDYPLAGAGFGTFQPIFQLYRHPATRKLWDHAHNDWLQAAAEGGVALP
                     LLMLGLLVVGWKRASASPGDVIGLAILAALVAIAVHSAVDFALRIPAIAVSTAFLVGC
                     ACGGDLAAPRRLWNHRSPGRVRSSESRCPTPPP"
     gene            5362..6357
                     /locus_tag="VD788_15130"
     CDS             5362..6357
                     /locus_tag="VD788_15130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV77645.1"
                     /translation="MPHTAALIATTLNCRSELERSLRSICREDNLERVEELVVVDGGS
                     DDGTVELLSDWARRVPSLRHVVSPGANISRGRNQAIESTRCEIIVCWDTGCEYPAGYV
                     PGMLQPFDDPRVDVVGARTIATGETPYERSLVRFYDRREASDRTNPSHRAIAYRRTVW
                     TRVGGYPEQVEAGEDSWFNARWHALGLHYELSPRAEVRWRVRGDWASTARMARRNARG
                     HFTLRVGAGRRILLTVTALYTSLVPAIVAAAWSALPLGCWVVGYAGYVAYRLSRGGRW
                     RGLVSPRDWFYGWAAMSALDVGTTVGVCQGLIAGRFGRIRSAVTARGGSLRRGSR"
     gene            6354..7859
                     /locus_tag="VD788_15135"
     CDS             6354..7859
                     /locus_tag="VD788_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77646.1"
                     /translation="MSGAGDGFGALDRWEIVRYVGLGLLLGLAGDVTDEFSIFGFVQF
                     GLLIVALAIGFVPTRHGLILLLAITIACRDLVQTSSESLELGRVTIASPWQAQVGLIR
                     PSWLVMVGMLLLLLRNPIYIGDRTVNRAIVWFASVPLLTGLVYGGLTGPHATIQVPVD
                     LKLPAFLMASILLYRSYLRRNPTLVATLVAVIVGCSVARHLLDLAYWGLDIGSMLAGV
                     NRVSVDSTKGTVVLLLIAGVFLIVTRRAVVLGAIVGVTSSILLAVYVTRMLWLTALLG
                     IIVVLLAQRTNRRVLVIPVAAAVLFVAIQLIETLNPDSMRLLEYRSNTLLGDPASLES
                     VDFVRYAEMVDSTATNARRFAVLWGSGYGGYYTDDWIPIPPYVPDSFPQYSLETGLFY
                     RCHGYVTHTLFKYGVVGLAVISALWVTPAWGCFQRLRRGRSELPDGIAVCMIAFMITA
                     MLQMSWTAKGYVLNGALIAICLSLYQSVAVEASRSRSPAVMPAVARVDAVT"
     gene            7856..9133
                     /locus_tag="VD788_15140"
     CDS             7856..9133
                     /locus_tag="VD788_15140"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXV77647.1"
                     /translation="MNHSIPAIFRRYRDNASNLISLVAVNFVTAGLGFVTNVALADHL
                     GSSGFGRLSYALAVGMVCMTVALFGMGRTFVREVIHFPDRARELVYGSFAVRVAVVAA
                     CAAGTLLLRRQLHLEPSQVLIVVATTLTALHLQGLYDAWGRIRLHAGYFFVQRAAYFA
                     LVWFLLALDPDELTLWSIGGGLLGATVLGLVLQYAWALPKLGGSAGVDVGAAARYLIR
                     SNLWVWLGAIAGLSFVNLNQIVVKHFHGDAALGRFAACLLLVNVSNLFLTQIARIGAP
                     GAARCTRPGVPLATRRAFLGKYLAVIVGASLVPALPCIVAPSRLLALFFTAEYGAEGT
                     TLRLLGFYVVAIGVALVATQFMVVLRLEKQFFVLVAVAGGLSLVTSLVLVPRWSGYGA
                     ALSLLVVHSLTILASLALVLRKLSSAATGEELV"
     gene            9146..10084
                     /locus_tag="VD788_15145"
     CDS             9146..10084
                     /locus_tag="VD788_15145"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV77648.1"
                     /translation="MPPWVVKAAVQGVLSRLPRAQRWNRVLQTYVTRSLFLSDDAFLS
                     KWRQARKHLEHYRMAHGSRTPRRALELGTGWAPVVPIGLALDGVPDVRTVDMQPLLSK
                     QLTLRVLEQYRRQIAAGAVAPADPRAGQRIGELLAAAPSSDAHGLLARIGVDVSVGDA
                     RASALPNGSVDLFVSNNTLEHIAPDVIREILSEFRRLAAGDAVGSHFIDMADHYAGFD
                     RRITVYNFLRYSDRRWRLYNNDLQFQNRLRLSEFRSLHRRTGWQIVHEESRDQPVEVL
                     RRVPLAERFRRYDENDLRVFESWIVSRPLGSQPAGG"
     gene            10304..10576
                     /locus_tag="VD788_15150"
     CDS             10304..10576
                     /locus_tag="VD788_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77649.1"
                     /translation="MLRMCAVGVGKTATALGAYYRRIAYRVGKAKAITATARKLAILV
                     YRVPGRDIDEAQHRTRTLRNLRNRAQRLGSGLIDLETGEVMPAEAS"
     gene            complement(10573..11709)
                     /locus_tag="VD788_15155"
     CDS             complement(10573..11709)
                     /locus_tag="VD788_15155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV77650.1"
                     /translation="MSRFHAGRIAIVYPNDGTDIRIGKTCRSLSAAGFDVHFVGWDRR
                     PGPPRAIELGGAERHVMTLGTAEARGDASGTLRFAGHVQRTLRRLRPEFASAVNEEYA
                     LLVLPLRRIAYRHLVCDIFDSLADRRSAAAVLSRWAARLICAAGRSGADRLIVTDEAR
                     LARLGRHRVKATVVANYPEDPGDELALVRPAGPTKVYVSGTLSAARGLREILEALESI
                     DDARIVAAGWPYDDYARRIFLTHDKVRYCGAISAASSLARAAECDAVFAFYAPDSLNN
                     RMASPNKVYDAMGVGRPVIINREVALSRWVEQHELGFVCPYHDRAALGRVLAALGERR
                     GRIASFAERARRLFKQGYSWARMEARLLELYGGLTQDRSIGTRS"
     gene            complement(11706..12830)
                     /gene="wecB"
                     /locus_tag="VD788_15160"
     CDS             complement(11706..12830)
                     /gene="wecB"
                     /locus_tag="VD788_15160"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HXV77651.1"
                     /translation="MKRTTVAVVAGTRPEAIKLAPVVRALAEHPRLEPKLILTGQHRE
                     LLDQALAAFGLDVAVDLDLMRPDQSLAETTARVVTGVGRTLREFAPCVVLVQGDTTTC
                     LGAALAAFYEKLPLGHVEAGLRTHDLTAPWPEEMNRRLVDPISRWCFAPTSRAADNLA
                     REGIAAERVFVTGNTVVDALLWARGLVEQRPPGVPGLDPEWVGGGRIVLVTLHRRESF
                     GEPLRELCRALADIGREHPDVTVVFPVHFNPHVRSAVEAELAGAPRVRLIPPLEYLPF
                     VHLLDRCHFVITDSGGIQEEAPSYGKPVLVARERTERPEAVDAGVARLVGTSRETIGR
                     AARQLLTDRSAYEAMVARGNPFGDGRAAERIVEVLDEATR"
     gene            12893..14119
                     /locus_tag="VD788_15165"
     CDS             12893..14119
                     /locus_tag="VD788_15165"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV77652.1"
                     /translation="MRILYFHQYFNTPEMSGSTRSYEIARRLVAGGHRVDIVTSDRGS
                     GASSNWRESECGGARVHWIAVPYSNELPFARRMRAFLRYALAAARRSSSIDADLIFAT
                     STPLTIALPAAYAKLRKRVPLIMEIRDLWPEVPIAMGVLRSRSSRALARALERFAYAC
                     SARVVALSPGMADGVARAGYPRERVVTLPNACDLELFDVPVSVGQAFRASHPWLGDRP
                     LVVYAGTLGRANDVGFLVRLAGSMRWIDERVRFLVVGDGAEREDLRALAAQLGVLDRT
                     LFMMDPVPKLAMPRILAAADLAVSTFARVPALWTNSANKVFDALASGTPIAINHGGWI
                     AELLDDHDAGLILDAADAEAAARRLAAALADGEWLARAATNARRLARDRFSRDRLVAQ
                     LEQLMREVVAESARAR"
     gene            14166..14795
                     /locus_tag="VD788_15170"
     CDS             14166..14795
                     /locus_tag="VD788_15170"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXV77653.1"
                     /translation="MLYAHSGKRALDVVVGLTLLVLLSPVIVAVAAAVRLALGSPVLF
                     RQVRPGLGAAPFELIKFRTMTRARGADGGLLGDGERITRLGRWLRRYSLDELPELWNV
                     LRGEMSLVGPRPLLTEYLALYTEEQARRHEVRPGITGLAQVGGRNRLDWESRFALDVW
                     YVDHVGLALDLKVLLLTLVRVLRADGISAAGHATTPPFVGSVRRTESDK"
     gene            14792..15928
                     /locus_tag="VD788_15175"
     CDS             14792..15928
                     /locus_tag="VD788_15175"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXV77654.1"
                     /translation="MSEATRGGIPMSSPDVDESDVLAVAEVVRSGRHALGPRTVEFEE
                     RLAAYVGASHAVAVSSGTAALHLIVRALGLGPGDEVLMPSFTFAATANAVLYEGATPV
                     FVDIDPETYNLDPDELARKATRNTRAVMAVDVFGHPAEWAAIEEFGRSRGIPLIDDSC
                     EALGAEYRGVRIGRFGTAAAFAFYPNKQISTGEGGIIVTDDQCLARQARSLRNQGRGE
                     MGAWLEHERLGYNYRMDEMSAALGLSQLNRIETFLGKRQRVADWYGSRLAGYDRVRAP
                     RVRDHVRMSWFVYVVTLAEGLEREPVMRRIERRGIPVRGYFSPLHRQPYITERFGSSD
                     GSLPVTESIARRTIALPFHNNLGQEEVSTVVTALVEAVEAVDEA"
     gene            15918..>17260
                     /locus_tag="VD788_15180"
     CDS             15918..>17260
                     /locus_tag="VD788_15180"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV77655.1"
                     /translation="MRPEPSDWLRRAGVQRGAPTLRELTVLALDGLITALALWLAMML
                     RFEGRITDVYLDVLPEFLLVLVGMRIVSSLGFRLHQWSFRFSSLTDGARIALAGLTGS
                     GLFLATMYLVRHPGPPRSVVVLELLLTTVLMAAVRFSPRLAGLYIGDWTRSRRQQSLR
                     TMIVGAGAGGEMLLRDLSRSSEHRYHVVGFVDDDRTRRRQVVGGKSVLGAIDDLPELI
                     RSQRVAQLLIAIPRLEGRRIREILSLCSEQKVRFKILPVSVAYLDQRPATAMMQDLTP
                     DDLLPRPQIEFSRSAIAGEIEQRTALVTGAAGSIGSEVCRQLLKAGLRRLVMVDMDEN
                     GLYLLKRGFEHRFADAELAVQVADVRDARRIRSLFDRYRPRDVFHAAAHKHVPLMELA
                     PCEAIKNNVIGTRNVASAAGDYGVERFVFISTDKAVRPTSVMGASKRIGEEIVRS"
BASE COUNT         2454 a         5946 c         5980 g         2880 t
ORIGIN      
        1 cgcccgccgc cgcgtcggcg ccgacgagca gcaggtcgcg acgcaccgtg gcacctggcg
       61 tcacgacgat cgccggtgtg gcgaccgggg cacgttgtcc ggccgggttg ccgataccca
      121 gtgcataccg gccggcgggg acgtgatcga aggcgtagac gccgtccgcg ccgacctcgg
      181 ccgaggcgac ctcgtggccg acgccgtcga tcaggtggac ggtgaacccg gcggccgtcg
      241 cgccgtctgc cgcgacgagc acgccgccga gccggccgcc gtccgcaccg acgccgaggg
      301 cgggtcggaa gagggcgagc agggcgagca ccgccgccgc gagacgacgt gccgcgctca
      361 ctgtccctcc gggtcgtcga gagactcgcc gtccggcggc gagaagatct cggagcgctt
      421 gaagatcgga tagaagggcc agccggcgag cttctccgtt cccccttcct cgcgatcgac
      481 gatacagacc actgcgacca ctgtgtggcc ctcggcctcc accgcctcga tcgccttgag
      541 cgtcgaggca gccgtggtca cgacgtcgtc gacgacgacc acgcgcgagc cgggctcgag
      601 gctgccctcg atccggcgcc cggtcccgtg ctccttggcc tccttccgca cgatgaaggc
      661 tcgcaacggc cggccgtcga gctgactcag cgctgcgacc ggacagacga tcgggtcggc
      721 gccgagggtc aggccaccga tcgcatcggc gcggatcgcc cgggcgcgca gcagctcgag
      781 cacctcgacc gcggccaggt aggcgccttc ggccagcagc gtcgtcttct tggagtcgaa
      841 gtagtaccgg ctcttcttgc ccgagctcag cgtgaagtcg ccgaagatca gcgagtgctt
      901 tcgcaggagc tccttcaacc gagatcgatc ccctttcaac atggtaattc ccgggttccg
      961 atacgtaatc gcctcacaaa gcccttgatc taacgggtcc tgttgtctat gttcgcctca
     1021 tgtgaggcgg cgcaattata gatatcggcg tcgaggagtt gaagagatga gagagcgaac
     1081 tgccggattc aagctggtcg cggcgctggt cgtcgtcggg ctgcagggcc tggcggtgcc
     1141 cgtgatggcc gacaaaccca cggccgaggt gcggggctcg gtgctttccg cgaccgacca
     1201 ggaaccggtc gccggggccc ggttgcacct gtcgaatccc gaaacgggag agttcttcag
     1261 ctccgcgccg acccgcgacg acggtggctt cgtgatcgag tccgttccgg tggcgaccta
     1321 cgacgcgggt gtcgagcacg acggcgggct gtacgtcatc gaatcaccgg tcagtatcgc
     1381 tcccggccag acccagccgc tccaggtagc gatcgacgag cagctcgccc cgtcccccga
     1441 cgagcaggaa gagcagcgca agaagagcgg caccagcgtc ctcaacaacc cggcgaccgc
     1501 cgccctgctg gttctcggcg cggcgatcgt gctcggcgtg atcatcgacg ccgcgaccga
     1561 cgacgacgac gagtcggcga gcccgttctg agcacccgtc cgcggagaac cgatgaccgt
     1621 ctcgaccggt gaccgttcgg cgtcacatgt cccgcgctaa cgaccacccg tctcgcaacg
     1681 agattcaatt gtgggatctg tggcgcatcg tggtgcgccg ccggaccctc gtcttcgcgg
     1741 cagtcgcgct gtcggtcgcg ctcactgccg tcgtgaccct gctctccacg ccgatctatc
     1801 aggcgacgac gaccatccag atcgaacgtc gcgggtcggg ggtgctcaat tacaaagacg
     1861 tcacgagcgt cgatccctcc tttgccggtt accaggactt ctaccaaacg cagtacagga
     1921 tcctccagag cgacgcggtc ctgcgcctcg ccgctcggcg gctcgatctg ccgaaccgcc
     1981 ccgactacgc cggacgcgag agcccgccga tcgcgcgcct cgtcggttgg gtcctgtcgg
     2041 cgctcccggc gccgtcgagg tcggaccgtg ctcgggacgg cgagaccgat gacccgcacg
     2101 atcaggcaac ttcgttcctg cgggcgcgcc tgtcggtcca accggtgcgt ggcagccagc
     2161 tcgtgcgggt ctcgttcctc gatcgggatc cggcgttggc gcgcgattcg gccaacgcgg
     2221 tggccgcggc ctaccaggag ttcagtctcc agtcccgcta ttcgacgacg gcgcaggcga
     2281 gcgagttctt gaccaagcag gtcgccgagc tgcagcagga agcggccgcg atggagcgtc
     2341 gtctccaggc actgagcgcg gagaaggaga tcttggcgct gcgggacgat acgaaggaca
     2401 tctcgacgct ggcgctgacc gatctgaacg ccaaactggt cgagtccaag gcgcgcctgg
     2461 cgctggccgg ggcgcgctac gacgcggtgc ggaccgcgcc cgccgactcg atggccgagg
     2521 tgctgcgcag cccgttgatc aacaacctca ggcagcagta cgcggaactc gagcgcaagc
     2581 accggcagat gtccgagcgg ttccgcgacg attggccggc gctgcgacag gtcaccgagg
     2641 agctgaccca ggccgcggcg cgcctcgagt tggaaaccga gacgatcgcc acccgggttc
     2701 gcggcgtggc tcgcgcggaa cgagaccagg ccggggccga ggtgcggttg ctccaagcgc
     2761 gggtcgacga acagaagcag gtggttcaac gggtcagctt ggacgccgtc gagatcgcga
     2821 gccttcaggc ggagatcaag accaagcggc ggttcctcga cgagctggtc aaccggcaga
     2881 accagacgga agtcaccgac cgtttgcgcg atccacaggc cagcaacatc cgggtggtcg
     2941 acgcggctcg cgcacccgac tacccggttc gtccgcgcaa gctgctgaat ctgctcgcct
     3001 cgctcgtcgc cgggctggtg ctgggcgccg gagccgctct gctctccgac catctggaca
     3061 acacggtgaa gaacgaggcc gacgtgcgca ttgccgcggg cctacccgtc ctcggtcaga
     3121 ttcgggtctt cgaatcgacc ttgacaccgt caccggtcgg cccgtcgccc acgcactggc
     3181 cggaccagcc gggactcggc tgccacgtcg agccgctgtc ggtgttcgcc gaggcgttca
     3241 agagcctgcg cacgtcgttg ctgctcgcgg cgcccgatca cccgccgcgc agcatcctga
     3301 tcacctccgc cgagccgggg gacgggaaat cgacggtggc ggtgaatctg tcgaccgtgc
     3361 tgacccagct cggcaagcgc gtactgctgg tcggagccga tctcagacga ccgagcgtcc
     3421 actcgatgct cgatctgccc aacaagcggg gtctgtccaa cctgctgacg ggaacggcgg
     3481 acctgcgcga cgtcgtctgt gagagcgccg taccgcggct cgacgtgata ccgagcggcc
     3541 cgatcccacc gacgccgtcc gagctgctgg gctcggccgg gatgggcgcg ctgatcgatc
     3601 gcctgctgaa cgacgagggc tacgaccacg tcgtgatcga ttccccgccg gcggcccagg
     3661 tgaccgacgc ggtcatcctg tcggcgtatg ccgccgcaac ggtcgtcacc gcccgcgccg
     3721 gtgccacgac gcgcgatgcg ctggcgctga cgctcgaacg gctgcggcgg gcgcgggcgc
     3781 atcttccggg gatcgtgctg aacggcgctc gggtcggcgg cgactatcgc taccgcctgt
     3841 actacggcga atcggccggc cccgagcacg gtcgcgggcc ggaacgcctc gtcccgcgcc
     3901 ggcgcgcctc gcgcaagcgc gccaccgggg tgtgacgacc ggcggcgggc cgcgatcgga
     3961 cgcggccacc gcgatcgcgt cgttcgttct gtttgcgctg ctgttggcgt ccccgctgcc
     4021 gttcggctct gtccagccac cctacgtgct cggcatcgaa ctcggcgcgg cgctggccgg
     4081 tggcctggcg gcgtggatcg tctggcgcga tcccgggcga ttgtccggcg cgggccgggt
     4141 cggtctggcg ttggccggcg cggtgctgct gatcggtgga gcgcagctgt tgccgctgcc
     4201 gcggagcctg gtcgccgtgc tcgccgggcc gacggtcgaa gcgcgggacg ggatcgcgct
     4261 ggcgatcgat gcaccgctcc cgcgttggat gccgttgacc ctctcgccac ccgccaccgt
     4321 cgatgcgctg ctccgctggt cggcctatct ggcgatcggc ttggcggcgg cggtctgcat
     4381 ccgttccgag cggcagcgac tcgtcgcggc cggtctgctg tcgctcgcgg gcgcgttcca
     4441 ggcgctctac ggttcggccg agtacctctc cggtcaccag cacatcttct ggtaccccaa
     4501 gcgctacttt accgacgagg cgagcggcac cttcatcaat cgcaaccact tcgccgccta
     4561 tctgtcgatc tgtcttccgc tggctgcggc ggcgacgctc gacggctacc gccgactgcg
     4621 cgcggtcggc gtccgctggc gagtcctggt attgctgacc gaggcgggag gctcgtgggt
     4681 ggtactcgcc gcggtcgcaa ccgcgtcgat ttggatcggc atcgtcctgt cgttctcgcg
     4741 cggcggtttg gcggcgtcgc tcgtcgggat cacgatcctg gtgggcggcc tgctacggcg
     4801 aaaacgcggg gcggtgtggc tgctggcgct gctcgcgatc ccgaccgcgg cgctgctcgc
     4861 gatggaagtc agggcgccgg gggcgcgatt cgtcaccgaa gcggaccacc tcgccgactt
     4921 cggtggacgc ctcgaggtat ggaccgccac cggcgagatc gtcggcgact acccgctggc
     4981 cggcgccgga ttcggcacgt tccaaccgat cttccagctc tatcgacatc cggcgacacg
     5041 caagctgtgg gaccacgccc acaacgattg gctgcaggcg gccgcggagg gtggcgtggc
     5101 gctcccgctc ttgatgctcg gtctgctcgt cgtgggatgg aaacgagcca gtgccagccc
     5161 gggtgacgtg atcggcctcg cgatcctcgc cgcgctggtc gcgatcgccg tccacagcgc
     5221 cgtcgacttc gccctgcgca ttcccgcgat cgcggtgtcg acggcgttcc tcgtcggctg
     5281 tgcatgcggc ggcgatcttg ccgctccccg gcgtctatgg aatcatcggt cgccgggccg
     5341 agtccggtcg agcgagagcc gatgccccac accgccgcct tgatcgcgac cacgctgaac
     5401 tgtcgcagcg agctcgagcg gtcgctgcgg tcgatctgcc gcgaggacaa cctcgaacgt
     5461 gtcgaggagc tggtcgtggt cgacggcggc tcggacgacg ggaccgtcga gctcttgtcg
     5521 gactgggcgc gacgggtccc gtcgctgcgc cacgtcgttt caccgggcgc caacatctcc
     5581 cgcgggcgca accaggcgat cgagtcgacg cgctgcgaga tcatcgtctg ctgggacacc
     5641 ggctgcgagt atcccgccgg ctacgtgccc ggcatgctgc agccgttcga cgacccgcgc
     5701 gtcgacgtcg tcggcgcgcg cacgatcgcg acgggcgaga cgccctacga acggagtctc
     5761 gtccgcttct acgatcggcg tgaggcgagc gaccgcacga atccttcgca tcgcgcgatc
     5821 gcctaccgac gcacggtctg gacccgcgtc ggcggctacc ccgagcaggt cgaagccggt
     5881 gaggattcgt ggttcaacgc ccgttggcat gcgctgggac tgcactacga gctgtcgccg
     5941 cgcgccgagg ttcgttggcg cgttcgcggt gactgggcct cgaccgcacg tatggcccgc
     6001 cgcaatgccc gcgggcactt cacgctgagg gtcggcgccg gccggcggat cctgctcacc
     6061 gtcacggcgc tctacacgtc gttggttccg gcgatcgtcg ccgcggcgtg gagcgcgctg
     6121 ccgcttgggt gctgggtcgt cgggtacgcg ggctacgtcg cgtaccggct gtcgcgcggt
     6181 gggcgctggc gcggactggt ctcgccgcgg gactggttct acggctgggc ggcgatgagc
     6241 gcgctcgacg tcggtaccac ggtcggtgtg tgccaggggc tgatcgctgg ccgtttcggc
     6301 cgcatccgat cggccgtcac cgcgcgcggc gggtcgctgc ggcggggatc ccggtgagcg
     6361 gggcggggga cgggttcggc gcgctcgacc gctgggagat cgtgcgctac gtcgggctcg
     6421 ggctgctgct gggactcgcc ggcgacgtga cggacgagtt ctcgatcttc ggtttcgtcc
     6481 agttcggcct gctgatcgtc gcgctcgcca tcggcttcgt cccgacgcgc cacgggctga
     6541 tcctgctgct cgcgatcacg atcgcctgcc gggacctggt gcaaaccagc tcggaatcgc
     6601 tcgagctggg acgggtgacg atcgcgagcc cgtggcaagc ccaggtcggt ctgatacgtc
     6661 cgagctggct cgtcatggtc ggcatgctgc tgctgctgct gcgcaacccg atctacatcg
     6721 gcgaccgaac ggtcaaccgg gcgatcgtct ggttcgccag cgtcccgctg ctgaccgggc
     6781 tggtctacgg cggcctcacc ggaccccacg cgacgatcca ggttcccgtc gatctcaagc
     6841 tgcccgcgtt cctgatggcc agcatcctgc tctatcggag ctacctccgt cgcaacccga
     6901 ccctggtcgc cacgctggta gcggtgatcg tcggctgctc ggtcgcgcgt cacctgctcg
     6961 atctggccta ctgggggctc gacatcggct cgatgctcgc cggcgtgaat cgcgtcagcg
     7021 tcgattcgac caagggaacg gtcgtgctgt tgctgatcgc cggcgtgttc ttgatcgtca
     7081 cccgccgcgc ggtcgtgctc ggtgcgatcg tcggggtcac gagcagcatc ctgctcgcgg
     7141 tctacgtcac gcggatgctg tggctgaccg cgctgctcgg gatcatcgtc gtcctgctcg
     7201 cgcagcgcac caaccggcgg gtgctggtga ttcccgtcgc cgcggccgtc ttgttcgtcg
     7261 cgatccagtt gatcgagacg ttgaacccgg actcgatgcg gctgctcgag taccgcagca
     7321 acaccctgct cggcgacccg gcctcgctcg agagcgtcga cttcgtgcgc tatgccgaga
     7381 tggtggactc gacggcgacc aacgcgcggc gcttcgccgt attgtggggc agcggctacg
     7441 gcggctacta caccgacgac tggattccga tcccgccgta cgttcccgat tcgttcccgc
     7501 agtacagtct cgagaccggg ctgttctatc gctgccacgg ctacgtcacc cacacgctgt
     7561 tcaagtacgg cgtcgtcggt ctggccgtca tcagcgcgtt gtgggtcact ccggcctggg
     7621 gctgcttcca gcgcctgcgt cgcgggcgga gcgagctacc ggacggaatc gcggtctgca
     7681 tgatcgcgtt catgatcacc gcgatgctgc agatgagctg gacggccaag ggctacgtgc
     7741 tcaacggcgc gctgatcgcg atctgtctgt cgctgtacca gtccgtcgcg gtcgaggctt
     7801 ctcggtcgag atcccccgcg gtgatgccgg cggtcgctcg ggtcgacgca gtgacgtgaa
     7861 ccactcgatc cccgcgatct tccgccgata ccgtgacaac gcgtcgaacc tgatctcgct
     7921 ggtcgcggtc aacttcgtca ctgccggtct gggcttcgtc accaacgtcg cgctggccga
     7981 ccacctcggc tcgagtggct tcggccggct gtcctacgcg ctcgccgtcg gaatggtgtg
     8041 catgaccgtc gcgttgttcg gcatggggcg caccttcgtc cgcgaggtga tccacttccc
     8101 cgaccgcgcg cgcgagttgg tctacgggtc gttcgcggtg agggtggcgg tcgtcgccgc
     8161 ctgcgcggcg gggacgctgc tgctccgtcg ccagctgcat ctcgagccct cgcaggtgct
     8221 gatcgtcgtc gcgacgacgc tgaccgcgtt gcacctgcag ggtctgtacg acgcatgggg
     8281 acggatccgg cttcacgcgg gctatttctt cgtgcaacgc gccgcctact tcgccctggt
     8341 ctggttcctg ctcgcgctcg atccggacga gctgaccctc tggagcatcg gcggcgggct
     8401 gctgggggcg accgtgctcg ggctggtgct gcagtacgcc tgggcgctgc ccaagctcgg
     8461 tggctcggcc ggcgtcgacg tcggcgccgc ggcgcgctat ctgatccggt cgaacctgtg
     8521 ggtctggttg ggcgcgatcg ccggcctgtc gttcgtcaat ctcaatcaga tcgtcgtcaa
     8581 gcactttcac ggcgatgccg ccctcggtcg cttcgccgct tgcctgctgc tggtcaacgt
     8641 gtcgaatctg ttcctgaccc agatcgcgcg gatcggtgcc ccgggcgccg cgcgctgcac
     8701 ccgaccgggc gtgccgctcg cgacgcgacg ggcgttcctc ggcaagtacc tcgcggtcat
     8761 cgtcggggcc tcgttggtgc cggcgcttcc ctgcatcgtc gcgccgagcc ggctgctcgc
     8821 tctgttcttc accgccgagt acggagcgga ggggacgacg ctccgcctgc tcgggttcta
     8881 cgtcgtggcg atcggcgtcg cgctggtggc cacgcagttc atggtggtgt tgcggctcga
     8941 gaagcagttc ttcgtcctgg tcgccgtcgc cggcggtctt tcgctcgtga cctcgctcgt
     9001 gctggttccg cggtggtccg gctacggcgc ggcgctgtcg ctgctcgtcg tacactcgtt
     9061 gacgattctc gcgtcgctgg cgctggtcct gcgcaagctg agctcggcgg cgaccggcga
     9121 ggagctcgtc tgagcgggag ggagaatgcc gccgtgggtc gtcaaagccg ccgtgcaagg
     9181 cgtcctctct cgactaccgc gggctcagcg ctggaatcgc gtgcttcaga cctacgtcac
     9241 ccgctcgctg ttcctgtcgg acgacgcctt cctgtcgaaa tggcggcagg cgcgaaagca
     9301 cctcgagcat taccgcatgg cgcacgggtc gcgcacgccg cggcgcgcgc tggagctggg
     9361 aaccggctgg gcccccgtcg tgccgatcgg gctcgccctc gacggtgttc ccgacgtgcg
     9421 gacggtcgac atgcagccgt tgctgtcgaa gcagctcacg ctgcgggtgc tcgagcagta
     9481 ccggcggcag atcgccgccg gcgcggtcgc gccggccgac ccgcgcgccg gccagcggat
     9541 cggcgagttg ctcgccgcgg cgccatcgtc ggacgctcac gggttgctcg cccgcatcgg
     9601 cgtcgacgtg tcggtcggcg acgcgcgcgc cagcgcgctg ccgaacggct ccgtcgacct
     9661 gttcgtctcg aacaacacgc tggagcacat cgcgcccgac gtcatccgcg agatcttgag
     9721 cgagttccgc cgactggccg ccggcgacgc ggtcgggagc catttcatcg acatggccga
     9781 ccactacgcc ggcttcgatc ggcgcatcac cgtctacaac ttcctccgct attccgatcg
     9841 ccgctggcgc ctgtacaaca acgacctgca gttccagaat cgactgcggc tctccgagtt
     9901 ccggtcgctg catcggcgca cgggctggca gatcgtccac gaggagagcc gcgaccagcc
     9961 ggtcgaggtg ctgcggcggg taccgctcgc cgagcgcttc cggcgatacg acgagaacga
    10021 tcttcgcgtg ttcgagtcgt ggatcgtctc gcggccgctc ggctcgcagc cggccggcgg
    10081 ctagtttccc gttttgatat tcgcagcctc cggcaccgac tcacgggtgc cgatctcacc
    10141 cagatcgacg ctctggggcc ctattccgca cagcgcttga tctcggagat cgggaccgac
    10201 atgagccgca ggcccaccga gaagcacttc acctccgggt tgacgctcgc accgaggaac
    10261 aagatctccg gccctcggcc aaccgcgccg ccgccatgcc gccatgctgc gcatgtgcgc
    10321 cgtgggtgtc ggaaagaccg cgacagcgct cggcgcctac taccgccgta tcgcataccg
    10381 tgtcggcaag gccaaggcca tcaccgccac ggcgcgtaag ctcgccatcc tcgtttaccg
    10441 tgtcccgggt cgcgacatcg acgaggctca gcaccgcacg cgcacactcc gcaacctacg
    10501 taaccgcgca caacgactcg gctcgggtct gatcgatctt gaaactggcg aggtcatgcc
    10561 agcagaggct tcctaagagc gggttccgat cgatcgatcc tgcgtcaagc cgccgtagag
    10621 ctcgagcagt cgcgcctcca tccgcgccca gctgtaaccc tgcttgaaca accgccgggc
    10681 ccgttcggca aacgacgcga tccgcccgcg acgctcgccc aacgcagcca gcacccggcc
    10741 gagcgccgcg cgatcgtgat acgggcaaac gaagcccagc tcgtgctgct cgacccaacg
    10801 ggacaacgcc acctcgcggt tgatgatgac cggccggccg actcccatcg cgtcgtagac
    10861 cttgttcggg ctggccatcc ggttgttgag cgagtccggc gcgtagaacg cgaataccgc
    10921 gtcgcactcg gcggcccggg ccagcgatga cgcggccgag atcgcgccgc aatagcgcac
    10981 cttgtcgtgg gtcaggaaga tccgcctggc gtagtcgtcg tagggccacc ccgcggcgac
    11041 gatccgcgcg tcgtcgatcg actccagtgc ctcgaggatc tcgcgcaagc cacgcgccgc
    11101 gctgagcgtt cccgagacat agaccttggt cgggcccgcg ggacggacga gggccagctc
    11161 gtcgcccggg tcctccggat agttggcgac gaccgtcgct ttgacgcggt gacgaccgag
    11221 gcgggccaag cgtgcctcgt cggtcacgat cagccggtcg gccccggagc gcccggcggc
    11281 acagatcagc cgagccgccc agcgcgacag aaccgcggcc gcgctgcggc gatcggccag
    11341 cgagtcgaag atgtcgcaga ccaagtgtcg ataggcgatc cgccgcagcg gcagcacgag
    11401 cagcgcgtac tcctcgttga cggcgctcgc gaactcggga cgcagccggc ggagggtccg
    11461 ttggacgtgg ccggcgaagc gcagcgtgcc ggacgcgtcg ccgcgggcct cggcggttcc
    11521 gagcgtcatc acgtgacgct cggcgccgcc cagctcgatc gcgcgcggcg ggcccggtcg
    11581 ccggtcccag ccgacgaaat ggacgtcgaa cccggccgcc gacagcgagc ggcaggtctt
    11641 gccgatgcgg atgtccgtgc cgtcgttggg gtagacgatc gcgatccgac ccgcgtggaa
    11701 ccggctcatc gcgtcgcctc gtcgagcacc tccacaatgc gctcggcggc acgtccgtcg
    11761 ccgaacggat tgccgcgggc gaccatcgcc tcgtaggccg agcggtcggt caacagctgt
    11821 cgcgcggctc ggccgatcgt ctcgcgcgag gtgccgacca ggcgggcgac accggcgtcg
    11881 accgcctcgg ggcgttcggt ccgctcgcgg gcgacgagca cgggcttgcc gtaggacggc
    11941 gcctcttcct ggatcccgcc cgaatcggtg atgacaaaat gacagcgatc gagcaggtgc
    12001 acgaacggaa ggtactcgag cggcgggatc agccgcacgc gcggagctcc ggccagctcc
    12061 gcttcgaccg cgctccgcac gtgcgggttg aaatggaccg gaaagacgac ggtcacgtcc
    12121 ggatgctcgc ggccgatatc ggccagtgcc cggcagagct cgcgcaacgg ttcgccgaag
    12181 ctctcgcgcc ggtgcaacgt gacgagcacg atccgaccgc cgccgaccca ctcggggtcg
    12241 aggccgggaa cgcccggcgg gcgctgctcg accaaccccc gggcccacag cagcgcatcg
    12301 acgacggtgt tccccgtgac gaacacccgc tccgccgcga taccctcgcg cgccaggttg
    12361 tcggctgcgc gagaagtcgg tgcgaagcac caacggctga tcggatcgac caggcgccgg
    12421 ttcatctcct cgggccaagg cgcggtcagg tcgtgggtgc gcagcccggc ctcgacgtga
    12481 cccagcggga gcttctcgta gaacgcggcg agcgccgcgc cgaggcaggt cgtcgtgtcg
    12541 ccttgaacca ggacgacgca cggcgcgaac tcgcgcagcg tgcggccgac cccggtgacg
    12601 acgcgtgcgg tcgtctcggc caggctctgg tcgggtcgca tcaggtcgag atcgacggcg
    12661 acgtccagcc cgaacgccgc gagcgcctgg tcgagcagct cgcggtgctg cccggtcagg
    12721 atcagcttcg gttcgagccg cgggtgctcg gcgagcgcgc gtacgaccgg cgcgagcttg
    12781 atcgcctcgg gacgcgttcc cgcgacgacg gcgaccgtcg tccgtttcac cgacccgttc
    12841 cttggaaggg cgccgggtga tggtgtagcg tacgcacctc ggagcgtcgc cggtgcgcat
    12901 cctctacttt catcagtact tcaacactcc ggagatgtcc ggcagcacac ggtcgtacga
    12961 gatcgcgcgc cggctcgtcg cgggcggtca ccgcgtcgac attgttacct cggaccgcgg
    13021 ctccggcgca agctcgaact ggcgcgagag cgagtgcggc ggcgcgcggg tccactggat
    13081 cgccgtgccc tattcgaacg agctgccgtt cgctcggcgg atgcgcgcgt tcctgcgcta
    13141 cgcgctggca gccgccaggc gcagctcgtc gatcgacgcg gacttgatct tcgccaccag
    13201 cactccgttg acgatcgccc tgccggcagc ctacgccaag ctgcgcaagc gcgtgccgct
    13261 gatcatggag atccgcgacc tgtggccgga ggtgccgatc gcgatgggtg tgctgcgcag
    13321 ccggtcgagc cgtgcgctgg cccgcgctct cgagcgattc gcctacgcct gctcggcccg
    13381 tgtcgtcgcg ctgtcgccgg ggatggccga cggcgtggcg cgcgccggct atccgcgcga
    13441 acgggtcgtc accctaccga acgcctgcga tctcgagctg ttcgacgtgc ccgtctcggt
    13501 cggacaggcc tttcgcgcga gccatccgtg gctcggcgac cggccgctcg tggtctacgc
    13561 cgggacgctg ggccgggcca acgacgtcgg ctttctcgtc cgcttggccg gatcgatgcg
    13621 atggatcgac gaacgggttc gtttcctggt cgtcggcgac ggggccgaac gcgaagatct
    13681 tcgtgccctc gccgcgcaac tgggcgtgct cgatcgaacg ctgttcatga tggacccggt
    13741 acccaagctc gccatgccgc ggatcctcgc cgccgccgac ctcgccgtct cgaccttcgc
    13801 ccgcgtcccc gcgctgtgga cgaactcggc gaacaaggtg ttcgacgcgt tggcttccgg
    13861 cacaccgatc gcgatcaacc acgggggttg gatcgcggag ctgctcgatg accacgacgc
    13921 gggattgatc ctcgacgctg ccgatgccga ggcggcggca cgacggctgg ccgcggccct
    13981 cgccgacggc gagtggctgg cgcgagcggc gaccaacgcc cggcggctcg cgcgggaccg
    14041 tttctcacgg gaccgcctgg tcgcgcagct cgagcagttg atgcgcgaag tcgtcgccga
    14101 gtccgctcgg gcccgatgat atgctagcgg cggttgggtg cgcgacggag gtgcgacccg
    14161 gttcgatgtt gtacgcccac agcggaaagc gagctctcga tgtcgtcgtc ggtctgaccc
    14221 tgctcgtgct gctctcaccg gtgatcgtcg ccgtcgccgc cgcggtccgt ctggcgctgg
    14281 ggtcgccggt cttgtttcgc caggttcgac ccgggctcgg cgcggctccg ttcgagctga
    14341 tcaagtttcg cacgatgacc cgggcgcgag gcgccgacgg cggcctgctc ggcgacggcg
    14401 agcggatcac ccggctcgga cgctggctcc gccgctacag cctggacgag ctgcccgagc
    14461 tgtggaacgt gcttcgcggc gagatgagtc tcgtcgggcc gcgcccgctg ctgaccgagt
    14521 atttggcgct gtacaccgag gagcaggccc ggcggcacga agtgcgcccg ggtatcaccg
    14581 gtctggcgca ggtcggcggc cgcaaccgac tggactggga gtcgagattc gcgctcgacg
    14641 tctggtacgt cgaccacgtc ggcctcgcgc tcgatctgaa ggtcctgctg ctgacgctgg
    14701 tacgagtact ccgcgccgac ggcatcagtg ccgcggggca cgcgacgacg ccgccgttcg
    14761 tcggcagcgt ccggcgaacg gagtccgaca agtgagcgaa gcgacgcggg gcgggatccc
    14821 gatgtcatcc cccgacgtcg acgagtccga cgtgctggcg gtggccgagg tcgtccgttc
    14881 cgggcggcat gcgctcggtc cgcggacggt cgagttcgaa gagcgtctgg cggcctacgt
    14941 cggcgcgagc cacgcggtcg ccgtcagctc cgggacggct gcgctgcatc tgatcgtgcg
    15001 cgccctcggt ctcgggccgg gggacgaggt gttgatgcca tcgttcacct tcgccgcaac
    15061 ggccaacgcc gtgctgtacg agggggcgac gccggtgttc gtcgacatcg atccggagac
    15121 ctacaacctc gaccccgacg agctggcgcg caaggcgacc cgcaacacgc gcgcggtgat
    15181 ggcggtcgac gtgttcgggc accccgccga gtgggcggcg atcgaggagt tcggtcgctc
    15241 gcggggaatc ccgctgatcg acgattcgtg cgaggcgctc ggcgccgagt accgcggcgt
    15301 gcggatcggg cgattcggca cggccgcggc gttcgcgttc tatccgaata agcagatctc
    15361 caccggcgag ggtggcatca tcgtcacgga tgatcaatgt ttggctcggc aagcgcgtag
    15421 ccttcgcaac cagggccgcg gggaaatggg cgcctggttg gaacacgagc gactgggtta
    15481 caactaccgg atggacgaga tgagcgcggc actcggtttg tcgcaactga atcgtatcga
    15541 gacgttcctg ggcaagcgcc aacgcgtggc cgactggtac gggtcgcggc tggcggggta
    15601 cgaccgggtt cgcgcgccgc gcgtccgcga ccatgtccgc atgagctggt tcgtttacgt
    15661 cgtgaccctc gccgaggggc tcgagcggga accggtgatg agacggatag agcggcgcgg
    15721 catcccggtg cgcggctact tctcgccgct gcaccggcag ccgtacatca ccgagcgctt
    15781 cggcagctcc gacggttcgc tgccggtgac cgagtcgatc gcgcgccgca cgatcgcgct
    15841 gccgtttcac aacaacctcg gccaggagga agtgtcgacg gtcgtcacgg cgctggtcga
    15901 ggcggtggag gccgtggatg aggcctgaac cgtcggattg gctgcgacga gcgggcgtgc
    15961 agcgcggcgc accgacgctg cgcgagctga cggtgctcgc gctcgacggc ctgatcaccg
    16021 cgttggcgtt gtggctggcg atgatgctgc ggttcgaggg caggatcacc gacgtgtatc
    16081 tcgacgtgct gcccgagttc ctgctggtgc tggtcggcat gcggatcgtc agcagtctcg
    16141 gctttcgtct gcaccagtgg tcgttccgct tctccagcct gaccgacggg gcacggatcg
    16201 cgctggccgg gctgaccggc agcgggctgt tcctcgcgac gatgtacctc gtccgacatc
    16261 cgggaccacc gcggtcggtc gtcgttctcg agttgctgct gaccacggtg ctgatggcgg
    16321 cggtgcgctt ctcgccgcgg ctcgccggac tctatatcgg tgactggaca cgcagtcgtc
    16381 gtcagcagtc gctgcgcacg atgatcgtcg gggcgggggc cggcggcgag atgctgctgc
    16441 gcgatctgtc gcgcagcagc gagcaccgct accacgtcgt cggcttcgtc gacgacgacc
    16501 ggacgaggcg tcgtcaggtg gtcggcggca agtcggtcct cggcgcgatc gacgatctgc
    16561 cggagctgat ccgctcgcag cgcgtcgcac aactgctgat cgccatcccg cggctggagg
    16621 ggcgacggat tcgcgagatc ctctcgctgt gctccgaaca gaaggtccgg ttcaagattc
    16681 tgccggtctc ggtcgcctat ctcgaccaac ggccggcaac ggcgatgatg caggacttga
    16741 cgcccgacga cctgctgccg cgaccccaga tcgagttcag ccgtagcgcg atcgcgggcg
    16801 agatcgagca gcgcacggcg ttggtcaccg gcgccgccgg ctcgatcggc tcggaggttt
    16861 gccgccaact gctgaaagcc gggctgcggc gactggtcat ggtcgacatg gacgagaacg
    16921 gcctctacct gctcaaacgc ggcttcgagc accgattcgc cgacgccgag ctggccgtgc
    16981 aggtggcgga cgtgcgcgat gcgcgccgca ttcgttcgct gttcgatcga tacagaccgc
    17041 gggacgtgtt ccacgcggca gcccacaagc acgttccgct gatggagctg gcgccgtgcg
    17101 aggcgatcaa gaacaacgtg atcgggacgc gcaacgtcgc cagcgccgcc ggcgactacg
    17161 gcgtcgaacg gttcgtcttc atctcgaccg acaaggcggt ccgaccgacg agcgtgatgg
    17221 gtgcgagcaa acgcatcggc gaggagatcg tgcgcagcat
//
LOCUS       DASPGN010000521         3469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1737277, whole genome shotgun sequence.
ACCESSION   DASPGN010000521 DASPGN010000000
VERSION     DASPGN010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737277"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VD788_15185"
     CDS             complement(<1..239)
                     /locus_tag="VD788_15185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77656.1"
                     /translation="MERLRQLWELPIVQQSVEIWLSGGWAMIALAVNALILFGIGFRL
                     WIRLRTQARSMPERKWRRWIDHPQERRGQVGELIE"
     gene            complement(246..1823)
                     /locus_tag="VD788_15190"
     CDS             complement(246..1823)
                     /locus_tag="VD788_15190"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV77657.1"
                     /translation="MMIGRSRNTRRPRTARIAAALVLLAPLAAWAQDDLSLESTQSAA
                     ERDLQQALAELSRLRDEIAADKLPLTRELATLETELIELRGAFQEQSRLLDTRTLDLN
                     NLQSEIDARQSEKTYLSGLFDEYVRNLETRLHVVELQRYRDELQSARLAPEAADHTPA
                     RVFEIQSGVIERSIDRLLDVVGGTSFAGTAIREDGLVEQVDFALLGPIALYATKDGTA
                     AGLAEQRLGSLEPGMTSFDDPAMVAEARAVVQTGTGELPLDPTLGNANRIEATQDTVR
                     EHILKGGPVMVPILLLAATALLVALGKWVQLARVRQPSTQRVERILAAVRKGEFTAAR
                     EQADRLHGPSGEMLRAGLEHVCEPKELVEEVMFEKTLETRLRLQSFLSFVAIAAAAAP
                     LLGLLGTVTGMINTFKLITVFGTGDAKTLSSGISEALITTEFGLIVAIPALLLHAYLS
                     RKAKRVTDGMEKLAVSFLNRIPSATPLPSDTPEKFADRVLEIIRRKIGEDYRAAPAGA
                     GAGGWPSLDPPGERPEH"
     gene            complement(1820..2605)
                     /locus_tag="VD788_15195"
     CDS             complement(1820..2605)
                     /locus_tag="VD788_15195"
                     /inference="COORDINATES: protein motif:HMM:NF023358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3450 family protein"
                     /protein_id="HXV77658.1"
                     /translation="MRRRLTHRLAGLAVVAATTGVLGQNAGGEDGSLVRSTRGTLAKW
                     VETQQILSKEKQDWQLAKQVLEQRIALMQSEIDTLEQRIAETAVSIGDADRKRQERIA
                     ENERLKQASTALARTIATLESKTRTLLATLPAPLAERIGPLAQRIPVDPQRTELSLTE
                     RFQNVIGILDQANKFNGDVTVVTETREVADGVTAEVQTIYLGLGQAYYVTANGESAGV
                     GVPSVEGWNWTRADELAPRVLEVIGIVKNEQVPAYVPLPVRVQ"
     gene            2809..>3469
                     /locus_tag="VD788_15200"
     CDS             2809..>3469
                     /locus_tag="VD788_15200"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXV77659.1"
                     /translation="MPAHPAVGRIADTLVDSVFSSLAGRLDSRGGPVYPLHIGDSWLD
                     PADGCRMEDLTVAAHPGLHRYTPTRGLPRLVHAIAARVAERSGIDTGPENVLVSAGAT
                     AGLTAVMHTILDPGDEVLILAPYWPLIRGIVLCAHGVPTPVPILGRVATSDELIEALE
                     RARTPRSVAVYLSTPNNPSGGVLPRGWVDGLADWATSRGLWIVSDEVYEDHVFEGEHR
                     PA"
BASE COUNT          499 a         1224 c         1164 g          582 t
ORIGIN      
        1 aactcgatca attcgccgac ctggccgcgc cgctcttgcg ggtggtcgat ccagcgccgc
       61 cacttgcgtt cgggcatgga acgcgcctgg gtccgaagcc ggatccacag ccggaagccg
      121 atgccgaaca ggatcagcgc attgacggcg agcgcgatca tcgcccaacc gccggaaagc
      181 cagatctcga ccgactgttg cacgatcggc agttcccaca gttgccggag ccgctccatg
      241 ccggatcagt gctccggtcg ctcgcccgga gggtcgagcg acggccagcc gcccgcgccg
      301 gcaccggcgg gtgcggcgcg gtagtcctcg ccgatcttgc ggcggatgat ctcgagcacg
      361 cgatcggcga acttctccgg cgtgtcgctc ggcagcggcg ttgcgctcgg gatccggttg
      421 aggaacgaca ccgcgagctt ctccatcccg tcggtgacgc gtttcgcctt gcgggacagg
      481 taggcgtgca gcagcaaggc cgggatggcg acgatcagac cgaactcggt cgtgatcagc
      541 gcctccgaga tgccggagga cagcgtcttg gcgtcaccgg tgccgaagac cgtgatcagc
      601 ttgaacgtgt tgatcatgcc ggtgactgtg ccgagcagcc cgagcagcgg cgccgccgcc
      661 gccgcgatcg cgacgaacga caggaagctc tgcaggcgca gacgcgtctc gagcgtcttc
      721 tcgaacatga cctcctcgac cagctccttc ggctcgcaca cgtgctcgag tcccgcccgc
      781 agcatctcac cgctcggccc gtggagccgg tccgcctgct cgcgggccgc ggtgaactcg
      841 cccttgcgca ccgccgcgag gatccgctcg acccgttggg tcgacggctg ccgaacccgg
      901 gccaactgga cccacttgcc gagggccacc agcaacgccg tcgccgccaa gagcaggatc
      961 ggcaccatca ccggtccgcc cttcaggatg tgctcgcgca ccgtgtcctg cgtggcctcg
     1021 atccggttgg cgttgcccag cgtcgggtcg agcggcagtt cgccagtccc ggtttgcacg
     1081 accgctctcg cctcggccac catcgccggg tcgtcgaacg aggtcatgcc cggctcgagc
     1141 gagccgaggc gctgctcggc caagcccgcc gccgttccgt ccttcgtcgc gtagagtgcg
     1201 atcgggccga gcagcgcgaa gtcgacctgt tcgaccaagc cgtcctcgcg gatcgccgtg
     1261 ccggcgaagc tcgtcccacc gacgacgtcg agcaaccggt cgatcgaacg ttcgatcact
     1321 ccgctctgga tctcgaagac ccgagccggg gtgtggtcgg ccgcctcggg tgcgagacgc
     1381 gccgactgca gctcgtcgcg gtaacgctgc agctcgacga cgtgcagccg cgtttcgagg
     1441 ttgcgcacgt actcgtcgaa caggccggag aggtaggtct tctcgctctg ccgcgcgtcg
     1501 atttcgctct gcaggttgtt caggtcgagg gttcgcgtgt cgagcaacct gctctgctcc
     1561 tggaacgcgc cgcgcagctc gatcagctcg gtctcgagcg tcgccagctc gcgcgtcagc
     1621 ggtagcttgt cggcggcgat ctcgtcgcgc agccgcgaca gctcggccaa cgcctgctgc
     1681 aggtcacgtt cggcggccga ttgcgtgctc tcgagcgaca gatcgtcctg cgcccaggcg
     1741 gcgagcggcg ccagcaggac cagcgccgct gcgatccggg cggtgcgcgg gcgtcgggtg
     1801 tttcgcgagc ggccgatcat cattggactc tcaccggcag cggcacatac gccggaacct
     1861 gctcgttctt gacgattccg atgacctcga gaacgcgtgg cgccagctcg tcggctcgcg
     1921 tccagttcca gccctcgacc gacggcacgc cgacgccggc ggactccccg ttcgccgtca
     1981 cgtagtaggc ttgaccgaga ccgaggtaga tcgtctggac ctccgcggtc acgccgtcgg
     2041 ccacctcgcg cgtctcggtg acgacggtca cgtcgccgtt gaacttgttg gcctggtcga
     2101 gaatgccgat cacgttctga aaccgctcgg tgagcgagag ctcggtgcgt tgcggatcga
     2161 ccggtatgcg ctgcgccagc ggaccgatcc gctcggccag cggcgccggc agcgtggcga
     2221 gcagcgtgcg cgtcttggat tcgagcgtcg cgatggtccg cgcgagcgcg gtcgacgcct
     2281 gtttgagccg ctcgttctcc gcgatccgct cctggcgctt gcggtcggcg tccccgatcg
     2341 agacggcggt ctcggcgatg cgctgctcga gcgtgtcgat ctcgctctgc atcagcgcga
     2401 tccgctgctc gagcacctgc ttggccagct gccagtcttg cttctccttg gagaggatct
     2461 gttgcgtctc gacccatttg gccagcgttc cgcgggtcga gcggacgaga ctcccgtcct
     2521 cgccgccggc gttttggccc aagacgccgg tggtggcggc aaccaccgcc agcccggcca
     2581 atcgatgcgt caagcgtcgt ctcatcggct cgatgcctcc ccgttcatgg gtcggttcta
     2641 ccgtcgggtt cggaggacgc gcgcggccgt cgccggctca cgacgataac cgagtccaca
     2701 atcagggttg ggtgattcga gtgacgaaac gatggcggtt tcgcgacgaa atcccgacaa
     2761 cccgtatcgg acaggctaga atcccgattc caccggagga ctccgtcgat gcccgcccat
     2821 cccgccgtcg gccgcatcgc cgacacgctc gtcgactccg tcttctcgtc gctcgccgga
     2881 cgcctcgact cgcgcggcgg tccggtctat ccgctgcaca tcggcgacag ctggctcgat
     2941 ccggcggacg gctgccggat ggaggacctg acggtcgccg cgcacccggg tctgcaccgc
     3001 tatacgccga cccgcggtct gccgcggctg gtgcacgcga tcgcggcgcg cgtcgccgaa
     3061 cgcagcggga tcgacaccgg gccggagaac gtgctcgtca gcgccggtgc gacggcggga
     3121 ttgaccgcgg tgatgcacac gatcctcgac cccggcgacg aggtgctgat cctggctccc
     3181 tactggccgc tgatccgcgg gatcgtgctg tgcgcccacg gcgtcccgac cccggtgccg
     3241 atcctcggac gggtcgcgac ctcggacgag ctgatcgagg cgctcgagcg ggcccggaca
     3301 ccgcgtagcg tggcggtcta cctgagcaca ccgaacaacc cctccggcgg cgtgctgccg
     3361 cgcggttggg tcgatgggct cgccgactgg gccacctcgc gcgggctgtg gatcgtatcg
     3421 gacgaagttt acgaggacca cgtgttcgag ggcgagcacc ggcccgcac
//
LOCUS       DASPGN010000522         5257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1737312, whole genome shotgun sequence.
ACCESSION   DASPGN010000522 DASPGN010000000
VERSION     DASPGN010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737312"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..738)
                     /locus_tag="VD788_15205"
     CDS             complement(76..738)
                     /locus_tag="VD788_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77660.1"
                     /translation="MKIRDRLPFRQPHPDSSPTEAEPQDAQQARHAEGPPREQPAAGH
                     PNGGLAGAFELVAQTVGDEIVALRDSLGGLEAKLMARLDVDRREASEAIETVRKDVLH
                     RFEEMRNSQQRALGEIAEKTRSAVAGIRDLVDQTREQVKDRSDEVRRGLEHILAEKEQ
                     RLAHELGSLSSDLAGVRLDLQHQMQASGRISNLLDNVANVFTGQPEPPRSIRPGSEDA
                     AS"
     gene            complement(984..1973)
                     /locus_tag="VD788_15210"
     CDS             complement(984..1973)
                     /locus_tag="VD788_15210"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HXV77661.1"
                     /translation="MRTREIAAAWAAEGCRIAQATVVRTWRSSPCPAGSLMVVDERGR
                     FAGSVSGGCIESAVIEEASRLIERGGSRSLEYGVTDERAWEVGLPCGGEVEILVGPFD
                     RGVNDRLLAARTERSSSALLIDLSTGVQRLVDPDELPGDLAEPEREFVTRHAHRERCG
                     VVDGRTFVQILGPPVRLILIGAVHLGQMVARLARLVGFEVSVVDPRPPFATPDRFPDA
                     RLVTKWPAEALAELGLDRRTAVVTLSHDAKLDEPALATALAAGCFYVGALGGRKTQQA
                     RRQRLRREGIDSAALARIHGPVGLDIGAVTAGEIAVAIVAQIVERWRRGGDER"
     gene            2097..2351
                     /locus_tag="VD788_15215"
     CDS             2097..2351
                     /locus_tag="VD788_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77662.1"
                     /translation="MADEGSGQKLLIGLLVGFGLGVAAALLLAPGGGARRRAALANAA
                     REVADRMTYVGRPDALVEKRTSRAGRALIDRLDRARSAGL"
     gene            2406..3764
                     /locus_tag="VD788_15220"
     CDS             2406..3764
                     /locus_tag="VD788_15220"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV77663.1"
                     /translation="MLKRFACSFLAVLAFPAAAFASGEAARYWPAWRGPSHDGVSPQG
                     DPPIVWSETQNVRFKVAIPGRSLSSPVVWGDRVFVMTAVAVDDAAYAASREAAADRAE
                     RGEWPPEVAPVEQRFLLLALSRDDGRVLWQREATRRAPHESHYVDSGWSTASPVTDGR
                     RVFAHFGSNGTYAFDVDGKPLWQIDLGDMTTRNSFGEGSSPALHDDTLVINWDHEGPS
                     FLVALDAATGEPRWKVERPDEVTSWSTPLVVQHGGRAQVVVSSTGRSRGYDLETGREL
                     WSLAGMTTNVIPSPVHRDGVVYLTSGFRGAMLQAVDLARATGPLEESNAVRWSHERDT
                     PYVPSPLLYDDSIYFLKGNKNILSVLDADTGKPRYEIKRLDALGDVWASPVAAAGRVY
                     VFDRDGKAVVLRHGPSFELLAENELDGTVNGSPAIAGGEIFVQTREHLYCIARDGAKS
                     VR"
     gene            complement(3771..4196)
                     /locus_tag="VD788_15225"
     CDS             complement(3771..4196)
                     /locus_tag="VD788_15225"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXV77664.1"
                     /translation="MNKQKMIDHLNEDLAHEFSAIIQYTIYAAMATGPYRPQLSQFFL
                     AEVADEQLHAQYLANKIVALGGEPVSTPEPLPKVKDNREMLVAILEAERGAVKRYSKR
                     AREAEELGDKGLQVQLEDMVRDESNHAEETERILQDWPV"
     gene            complement(4269..4991)
                     /gene="mtgA"
                     /locus_tag="VD788_15230"
     CDS             complement(4269..4991)
                     /gene="mtgA"
                     /locus_tag="VD788_15230"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HXV77665.1"
                     /translation="MRTKFAWRRRRWRRAAVPAAILASWLAWEAARWPAVGPLVAENP
                     RTTAYIDRFRAERRGRGEPADVAQVWVRYDRISRQLKKAVLVAEDINFFSHSGFETAE
                     LREAMRRAVTKRELPRGASTITQQLARNLWLSPSRNPLRKLREALLTRQIERTLSKER
                     ILELYLNVAEFGPGIYGVEAAARYYFGRSARRLDRHQAARLAATLPAPSRLNPATIAR
                     DHWRVKMILNRMEKAEFLDRRL"
BASE COUNT          744 a         1874 c         1806 g          833 t
ORIGIN      
        1 aatacgatga ggggccggac gcgcatggtg tcgctccggc gtcattctag accggatcgc
       61 ggccggtttg tcgggttacg aggccgcgtc ttccgagccc ggacggatgg accgcggcgg
      121 ttccggctgg ccggtgaaga cgttcgccac gttgtcgagc agattcgaga ttcgtcccga
      181 ggcctgcatc tggtgctgca ggtcgagacg cacgccggcc agatcgctcg atagcgaacc
      241 gagctcgtgg gccaggcgct gctccttctc cgccaggatg tgctccaacc ctcgccgcac
      301 ctcgtcgctg cggtccttga cctgctcccg cgtctggtcg acgaggtcgc ggatcccggc
      361 gacggcggac cgggtcttct ccgcgatctc gcccaacgcc ctctgctggc tgttgcgcat
      421 ctcctcgaac cggtgcagca cgtctttgcg caccgtttcg atcgcctcgc tcgcctcgcg
      481 ccggtcgacg tcgagccgcg ccatcagctt ggcctcgaga ccgccgagcg agtcgcgtag
      541 tgcgacgatc tcgtcgccga cggtctgcgc gaccagctcg aacgcgccgg ccagaccgcc
      601 gttcgggtgt cccgcagccg gttgctcgcg cgggggtccc tcggcgtgtc gcgcctgctg
      661 cgcgtcctgc ggctcggcct cggtcggact cgagtcggga tggggttggc gaaacggaag
      721 gcggtcgcga atcttcatgg cgtgcccctc ctggcgcgtg atcgaactct cgccgggtaa
      781 acgtaggctt cgcggaacgg ccgggcaaag ccaagttcga atgccgggtt acacgatcgt
      841 cgttgcgcct tttttacggg gtttgtacgc cccgggcgac ccgagacggg cgaggcgatg
      901 ggcgcgggcc gagacgaccg cgccgcacgc acccggcgtc tgctcgagct gtggcgcgcc
      961 gaggtcgggc tgcctgcgat cgactagcgt tcgtcgccgc cgcgacgcca ccgctcgacg
     1021 atttgcgcca cgatcgccac cgcgatctcg ccggccgtca ccgcgccgat gtcgagaccg
     1081 accggaccgt ggatcctcgc cagcgccgcc gagtcgatgc cttcccggcg cagccgttgt
     1141 cgtcgtgcct gttgcgtctt gcggccgccg agcgcgccga cgtagaaaca accggcggcc
     1201 agcgccgtcg ccaaggccgg ctcgtcgagc ttggcgtcgt gcgacagcgt cacgaccgcc
     1261 gtacgccggt cgaggcccag ctcggccagc gcttcggccg gccacttggt caccagtcgc
     1321 gcgtcgggaa agcggtcggg cgtggcgaac gggggacgcg ggtcgaccac gctgacctcg
     1381 aatccgacca gccgggccaa acgggccacc atctgtccga ggtgcacggc gccgatcagg
     1441 atcagccgca ccggcggacc gaggatctgc acgaacgtcc gcccgtcgac gaccccgcac
     1501 cgctcccggt gagcgtggcg cgtgacgaac tctcgctcgg gctcggccag gtcgcccggc
     1561 agttcgtcgg ggtccaccag acgctggacg ccggtcgaga ggtcgatcaa caacgccgac
     1621 gacgatcttt cggtgcgagc ggcgagcaac cggtcgttga ctccccggtc gaacggaccg
     1681 accaggatct cgacctctcc gccgcacggc agccccacct cccacgcacg ctcgtcggtg
     1741 acgccgtact cgagcgagcg cgatccgccc cgttcgatca gccgcgacgc ctcctcgatc
     1801 acggccgact cgatgcagcc gccggagacg gagccggcga accgccctcg ctcgtcgacg
     1861 accatcaggc tgcccgccgg gcacggcgac gaacgccagg tgcggaccac cgtcgcctgg
     1921 gcgattcggc acccttccgc ggcccacgcc gcggcgatct cgcgggtcct cacgtcgtcc
     1981 atctccaacc cggagtccat cctgcgccgg gccaagagta tcaccctcgg gaggcgcgtc
     2041 cggcgccggg cggcgcgatg ctatgatccg cagagacgac ggcgcgaggt ggtctgatgg
     2101 ccgacgaggg ctccggacaa aagctgctga tcggcttgct cgtcgggttc ggcctggggg
     2161 tggccgccgc gttgctcctc gcacccggcg gcggtgcccg gcggcgagcc gcgctggcca
     2221 acgcggcgcg ggaggtcgcc gaccggatga cctacgtcgg ccgtccggac gcgctggtcg
     2281 aaaagcgaac ctcgcgtgcc gggcgggcgt tgatcgaccg gctcgaccgg gcgcgctccg
     2341 cagggctctg atcaccgctt cgcggtcggt tggttggcgc gtgccaagtg tggcatcctc
     2401 gacccatgct caagagattc gcgtgttcgt tcctcgcggt cctcgccttc cccgccgccg
     2461 ccttcgcttc cggcgaggcc gcgcgctact ggccggcctg gcgcggcccg tcgcacgacg
     2521 gcgtcagccc acagggtgat ccgccgatcg tctggagcga gacgcagaac gtccggttca
     2581 aggtcgcgat ccccggtcgc agcctgtcgt cgcccgtcgt ctggggcgat cgggtcttcg
     2641 tgatgacggc cgtcgccgtc gacgacgcgg cctacgccgc gtcccgcgag gccgctgccg
     2701 accgggccga gcggggcgaa tggccgcccg aggtcgctcc ggtcgagcag cgcttcctgt
     2761 tgctcgcgct ttcgcgcgac gacggacggg tgctgtggca acgggaggcg acgcggcgcg
     2821 ccccgcacga gagccactac gtcgattccg gctggtcgac ggcttcgccc gtgaccgacg
     2881 gacgtcgtgt cttcgcgcac ttcggctcga acggcaccta cgccttcgac gtcgacggga
     2941 agccgctgtg gcagatcgac ctgggcgaca tgacgacccg caactctttc ggcgaaggca
     3001 gctcgccggc gctccacgac gacacgctgg tgatcaactg ggaccacgag ggtccgtcct
     3061 tcctcgtcgc gctcgatgcc gcgaccggcg agccacggtg gaaggtcgaa cgccccgacg
     3121 aggtcacgtc gtggtcgacc ccgctcgtcg tccagcacgg cgggcgcgcg caggtcgtcg
     3181 tctcgtcgac cgggcgcagc cgcggctacg acctcgagac gggtcgcgaa ctgtggagcc
     3241 tcgcggggat gacgaccaac gtgatcccgt cgccggtgca ccgcgacggc gtcgtctacc
     3301 tgacgagcgg tttccgtggt gcgatgctgc aagccgtcga tctcgcgcgc gcgaccgggc
     3361 cgctcgagga atcgaacgcc gtccgctgga gccacgaacg cgacacgccg tacgtcccgt
     3421 cgccgctgct gtacgacgat tcgatctact tcctcaaggg caacaagaac atcctgtcgg
     3481 tgctcgacgc cgacaccggc aagccgcgat acgagatcaa gcggctcgac gccctcggcg
     3541 acgtctgggc ctccccggtc gcggcggcag gccgggtcta cgtcttcgat cgcgacggca
     3601 aggcggtcgt gctgcggcac ggaccgagct tcgagctgct cgccgagaac gagctcgacg
     3661 gcacggtcaa cgggagcccg gcgatcgccg gcggcgagat cttcgtgcag acacgcgaac
     3721 acctctactg catcgcgcgc gacggggcga agagcgtgcg ctgagccgcg tcagaccggc
     3781 cagtcctgca ggatgcgctc ggtctcctcc gcgtgattgc tctcgtcgcg caccatgtcc
     3841 tcgagctgga cctggagacc cttgtcgccc agctcctccg cctcgcgggc gcgcttgctg
     3901 tagcgcttga ccgccccgcg ctcggcctcg aggatcgcca ccagcatctc gcggttgtcc
     3961 ttgaccttcg gcagcggctc gggggtcgac accggctcgc cgcccagcgc gacgatcttg
     4021 ttggccaggt actgcgcgtg gagctgctcg tccgcgacct cggcgaggaa gaactgcgac
     4081 agctgcggcc ggtacggccc cgtggccatg gcggcgtaga tcgtgtactg gatgatcgcg
     4141 ctgaactcgt gggccaagtc ttcgttgaga tgatcgatca tcttctgttt gttcatggcg
     4201 tgtgtccttt cttcgattcg atgatcatag ccaatcggcc gccgttctct caaccgcggc
     4261 ccgcgccgtc acaggcgacg atcgaggaac tcggcctttt ccatccggtt caggatcatc
     4321 ttcacccgcc aatggtcgcg ggcgatggtg gcgggattca gccgtgacgg cgccgggagc
     4381 gtcgcggcca agcgcgccgc ctggtggcgg tcgagccttc gcgccgagcg accgaagtag
     4441 taccgcgcgg cggcctcgac cccgtagatc cccggtccga actccgccac gttgagatac
     4501 agctcgagga tccgttcctt ggagagcgtg cgctcgatct gacgcgtcaa cagcgcctcg
     4561 cgcagcttgc gcagcgggtt gcgcgaaggt gacagccaca ggttccgcgc cagctgttgc
     4621 gtgatcgtcg acgccccgcg cggcagctcc cgctttgtca ccgcccggcg catcgcctcg
     4681 cgcagctcgg cggtctcgaa gcccgagtgg gagaagaagt tgatgtcctc ggcgacgagg
     4741 accgccttct tgagctggcg cgaaatgcga tcgtaccgca cccagacctg tgccacgtcg
     4801 gcaggctcgc cgcggccacg acgctcggcg cggaagcgat cgatgtaggc cgtggtgcgc
     4861 ggattctcgg cgaccagcgg cccgaccgcg ggccagcgcg cggcttccca cgcgagccac
     4921 gacgcgagga tcgccgccgg cacggcggca cggcgccagc ggcggcggcg ccacgcgaac
     4981 ttcgtccgca agccggtttc gttcgacgga tgcacgcgca aggccctccc cgagacggcc
     5041 gccaccgacg atcatcggcg gatcgcggcg ctgcggcaag ctctcgccgc gccgaccggg
     5101 ttccgctatc ttgctgattc acaccggggt ctggagtggg ggaccgatga cgagaccagg
     5161 ctttagcggg cgggcttcga tcggatcgct ggcgctgctg gcactgctgc tgtcgccggc
     5221 ggcgggcgcc gccaagaagg acaagaaggc cgaggac
//
LOCUS       DASPGN010000523         2640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1620374, whole genome shotgun sequence.
ACCESSION   DASPGN010000523 DASPGN010000000
VERSION     DASPGN010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620374"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2356)
                     /locus_tag="VD788_15235"
     CDS             complement(<1..2356)
                     /locus_tag="VD788_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77666.1"
                     /translation="MIDPAQRVEPSRAGLGRGPTGAGAPTAVPALAHDEIRFGTPLRL
                     ERTYVGAIDTVLALTARAAAPTALASADFDRDGIADLVCAYARGDEGALALHRGNVDA
                     LFPKDPAAQRRLADGTFSGEPFLSPASVTRVGLRPDLVAAGDFDADGTADLVIASRDA
                     GRLVLLPGDGAGSFGTFRTLDLPGRATALFAGEFGRPDGLPELIVGIDGDDGPTVRIF
                     DGRTGAFAADPRTLALSDPATDLTTDQLDGRPGIDLAVTAGNELIVVYGGERLDRRYR
                     VDRVRLRSAARSVDSGRFVPAPRAGERQLAVVLDDASLLLFGLADGRWEPLDERRLAA
                     AASAGPPPSTARVPGRVTDHLLLLRPEQRLELHRVGPADDREAGAGTPAVGAIDVGGD
                     PVAVLPMRLDPDGLADLVVLDSTGDPIRVQAIRGVPFVVTNTNDAGAGSLRQAMLDSN
                     AAAGADDITFDIPGGGPHSIVLSSPLPAITDTVQIDGATQPGFAGTPLVSLVGNGAGD
                     ANGLTLAAGSDGSVVRSLNIRGFSVTPTCNLSGAICVSDADCPLAGDFCDDKGLGRGI
                     VIRSNTNRIEGNFVGTDVTGMAAAPNLAGLVVDVNAQDNAIGGTVAAARNVISANDGV
                     GIGIDVGAANNLIRGNYVGVDAAGNADLGNAFSGMLIFANNNVVGGDLAGAGNVIAGN
                     DEFGVAIAFDAAFETLVQGNRIGVNAAGTGAIANSFSNLLVFDSSRTQIGGTTASAGN
                     VISGSNESGVFILDGDPSVPPTPTANRVEGNRIGTNAAGTAALGN"
BASE COUNT          387 a          925 c          948 g          380 t
ORIGIN      
        1 cgttccccag cgctgcggtt cccgcggcat tggtcccgat ccggttcccc tcgactcgat
       61 tggcggtcgg cgtcggcggg accgacgggt cgccgtcgag gatgaacacc cccgactcgt
      121 tgctgccgga gatgacgttt cccgcgctgg cggtagtacc tccgatctgg gtccgcgacg
      181 agtcgaacac cagcagattg ctgaagctgt tggcgatcgc gccggtgccg gcggcgttga
      241 caccgatgcg gttgccttga accagcgtct cgaacgcggc atcgaatgcg atcgcgacgc
      301 cgaactcgtc gttgccggca atcacgttgc ctgccccggc gaggtcgcct ccgacgacgt
      361 tgttgttggc gaagatcagc atcccgctga acgcgttgcc cagatcggca ttgcccgcgg
      421 catcgacacc gacgtagttc ccgcggatca ggttgttcgc cgcgccgacg tcgatgccga
      481 tgcccacacc gtcgttggcc gagatcacgt tgcgcgccgc cgccacggtc ccgccgatcg
      541 cgttgtcctg ggcgttgacg tcgacgacga gtccagcgag gttcggggcg gcggccatgc
      601 cggtcacgtc ggtcccgacg aagttccctt cgatgcggtt cgtgttcgac cggatcacga
      661 tcccgcgtcc cagacccttg tcgtcgcaga agtcgccggc cagcggacag tcggcgtcgg
      721 atacacagat cgcgccggac aggttgcagg tgggcgtgac ggaaaagccg cggatgttga
      781 gcgatcgaac gacgctcccg tccgaaccgg cggcgagcgt cagcccgttc gcgtcgccgg
      841 ccccgtttcc gaccagcgag acgagcggcg tcccggcgaa gcccggctgc gtcgctccat
      901 cgatctggac ggtgtccgtg atcgccggca gcggcgacga caagacgatc gagtgaggcc
      961 caccacccgg tatgtcgaag gtgatgtcgt cggccccggc ggcggcgttg ctgtcgagca
     1021 tcgcctgtcg cagtgagccg gcaccggcat cgttggtgtt ggtgacgacg aacgggacgc
     1081 cgcggatcgc ctggacgcgg atcggatcgc ccgtcgaatc gagtacgacc aggtcggcga
     1141 ggccgtcggg gtccagccgc atcggcagca cggcgaccgg gtccccgccg acgtcgatcg
     1201 ctccaaccgc cggcgtgccg gcgcccgctt cgcggtcgtc ggccggcccg acgcgatgca
     1261 gctcgagacg ctgctccggt cgaagcagca gcaggtggtc ggtgacgcgg cccggcaccc
     1321 gtgcggtcga cggcggcgga ccggcactgg ccgcggcggc gagccgccgc tcgtcgagcg
     1381 gctcccagcg gccgtcggcg agcccgaaca gcagcaggct cgcgtcgtcg agcacgacgg
     1441 ccaactgccg ctcgccggcc cgcggcgccg ggacgaagcg ccccgagtcg accgaccgag
     1501 cggcggaacg aagacgcacg cgatcgacgc ggtatcgccg atcgagacgc tctccgccgt
     1561 agaccacgat cagctcgttg cccgcggtca cggccaaatc gatccccgga cggccgtcga
     1621 gctggtcggt cgtcaggtcg gtggccgggt ccgacagcgc gagcgtgcgg ggatcggcgg
     1681 cgaaggcacc ggttctgccg tcgaagatgc ggacggtcgg accgtcgtcg ccgtcgatgc
     1741 cgacgatcag ctcgggcagg ccgtcgggac gtccgaactc gccggcgaac aacgcggtgg
     1801 cgcggccggg aagatcgagg gtgcggaacg tcccgaagct ccccgcgccg tctcccggaa
     1861 gcagcaccag gcgcccggcg tcacgcgagg cgatcacgag gtcggccgtc ccgtcggcgt
     1921 cgaagtcgcc ggccgcgacg agatcgggtc ggagccccac ccgcgtcacg ctcgccgggg
     1981 aaagaaacgg ctcgccggag aaggtcccgt ctgccagtcg tcgctgcgcc gccggatctt
     2041 tcgggaacag cgcgtcgacg ttgccgcgat ggagcgcgag cgcgccttcg tcgccgcgag
     2101 cgtaggcgca gacgaggtcg gcaatgccgt cgcgatcgaa gtccgccgat gcgagcgcgg
     2161 tcggtgcggc cgcgcgcgcc gtcagcgcca ggaccgtgtc gatcgcgccg acgtaggtgc
     2221 gttcgagccg gagcggcgtg ccgaagcgga tctcatcgtg cgccagcgcg ggcacggcgg
     2281 tcggcgcgcc ggcgcccgtg ggcccgcgac cgaggccggc tcggctcggc tcgacccgct
     2341 gtgccgggtc gatcatcgtt cgggtctcgt cggcgttccc cgtctgaccg ttcggctccg
     2401 tcgccggcac agatccagcg aggaaaacga ggacgagaca aaccacctgg atccgggcct
     2461 tcgtcatgga accccctttc ggtccacgac gggatcgccc gcgcggcggt tcgcaccgca
     2521 cccggttggt cacacgaccg cgccccgccc gtccggaacc gtttgggcga taggatacgt
     2581 cgaatcgtgc gccgggggag atgcacccgg cgaaatttcg gtcggcccgc ggcgacggcc
//
LOCUS       DASPGN010000524        11179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_800947, whole genome shotgun sequence.
ACCESSION   DASPGN010000524 DASPGN010000000
VERSION     DASPGN010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11179
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800947"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2615
                     /locus_tag="VD788_15240"
     CDS             <1..2615
                     /locus_tag="VD788_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77667.1"
                     /translation="TITDPDGYSETQQISMKTRAEPPTAGIATSTPSTLSQIRAAISS
                     ASPGSTIEIQPGVYTASSADGEVLDISNLRGTATNPITIRCASRDTTTLDANNNWYGI
                     YGGGTIEYVHIENCTIANYRRWDNAESDESMACVYLNAHTKGVVFRSNRCSGQGNGAI
                     IGPYKANFGNWISNNTFDGPIAFNSGQPSDHVGIEIMGQGHVVRNNTLTNMGDALNLG
                     RAENATNWPPDQPTHRANEFSLNEIDVAGDDCMEFDGGESNNLAWQNRCTNVVSCFSW
                     QPTNGANNVAARNVCLPWMGRYALKPGNETWGPVAYNNLFGLPWIMFQPNGFAADIRN
                     NLWVGNPDRAKRSVANIFATSLKLCRDSRVENGSTAVDNCNGPLAFWNDYNAYNYDGP
                     YDLAPIDEPNFAAAKAAAPEYEVGVRQMQHDVLISPGNASDIFADQNYSFPDTASCGC
                     SGSPSVCDCDAWTNNDVQPRIDASPKAGAQHVDAGTDLGAFTAGFSGNAPDIGPIELG
                     QAVPAYGADWSSADPPDPPSPPTSLFAVAVSSTRIDLSWSTPQGGGPVAYYKLYRDDV
                     LVGQTGAISFSDVGLSEATSYSYYVTSVGGGLESDPSSTVVATTLVDTTAPAIQSVRA
                     DGDPTRVFVVFTEPVEQSSASNSSNYAIDNGIAITSAALSPDLVTVVLSTTVHIENVM
                     YTLTVDDVRDRSANANPIAPGTTASYLYSAVLELSNLNVLSGKSYQVVENLANGDLVY
                     IDRSFTYSQVPTYLLGSTYIRTANDDKLSDGNVDFLSFDVNRDVTVFVAHDDRYTIKP
                     GWLADFVDTGDNVVSTSTFSLFRRDYPRGTIVLGGNVHPNEAEDNAMYTVIFVGSTFD
                     PGNPPPEPSNPRVH"
     gene            complement(2641..3981)
                     /locus_tag="VD788_15245"
     CDS             complement(2641..3981)
                     /locus_tag="VD788_15245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HXV77668.1"
                     /translation="MRDILLVVVVFGALPIVLLRPQIGVILWCWLSYMNPQTLAHGLA
                     RGMRFAAFVAVALIAGLLISGERKRLPRSPVVGLWFLWVGWQCITTWLAFYPTIAEVA
                     LNKTLKIQLITLCIMLIMWSRPRIDATVWVIVLSLGYFGVRGGLMTLVTGGQYRVWGP
                     DGGFIAGNNELGLALTMTLPLMRYLQLSTRTHWIRLGLLGVMVLTVVAIIGTYSRGGF
                     LAASAMGIVLALKSRHRLRLLVVGAVLIPLALGLMPERWFERMGTIKTYEQDASAMSR
                     INSWNFAINLANDNPVFGGGYGTFQAPMFAKYAPDPTIVYDAHSIYFEVLAEHGYGGL
                     AIFVALAFASWRAATRTAAAAANNPNLIWAHDLSRMLQVSIVGYAVGGAFLGLAYFDL
                     FYNLIALVVLTQAVVREGTAAVHAGGPSVEGREVNPGTRPAPSSLSPSRAGQRR"
     gene            4294..5361
                     /locus_tag="VD788_15250"
     CDS             4294..5361
                     /locus_tag="VD788_15250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HXV77669.1"
                     /translation="MVGSESRLGAAACVIRELDEPAATGWDRFVLDHPAGTFFHRAGW
                     KQVIERAMGHRCRYAYAERDGRIVGVLPLAHVKTALFGNRLVSTPFCVYGGPLATDET
                     VRRALTEHAIRIQDELQVDWLEFRSRERTQPGWTSQGDLYSTFRKEIPTGEQEILKSI
                     PRKARAVVRKAIDDDALEERTDETPDRFHHVYSVSVRNLGSPVFSRKYCRWLKQAFGD
                     DCEFFVLATRGGLPVAGVLSFYFRDEILPYYGGGTEAAREYGAHAYMYWRLMVRAAAR
                     GVRVFDFGRSKRGTGAYSFKTNFGFSPDTLLYEYRMKEGVSLPDATTLNPKYRSLIAT
                     WKRLPLPIANAIGPFLVRRLG"
     gene            5371..6573
                     /locus_tag="VD788_15255"
     CDS             5371..6573
                     /locus_tag="VD788_15255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HXV77670.1"
                     /translation="MEHLVFISQRIPYPPNKGDKIRSWNALIRLAERYRVHVGSFVDD
                     PEDWGHADKIGEISASYRLLPLRRTAKLWSLRGLATGEALSLPYFRDRRLVRWVESTR
                     ERYAPRAAFTFSSGVAGYVMGPRWAGVRRVHDMADVDSDKWLQYAKKKSGVARMIYSR
                     EGRKLLAFERRVAAEYDATLFVSEDEAAMFRRLAPESAGRIHALHNGVDADYFDPAVG
                     HDDPFPAEITPIVMTGTMDYWPNVDAAQWFADEALDEVRRIIPNAAFYVVGANPTRTL
                     RALGERPHVTVTGRVPDVRPYLAHARFVVAPLRIARGVQNKVLEGMAMARPVLASAMA
                     LEGIRAEIGSEVLLADSAADFARHAASVDEATGAAIGGRARARVLASYSWNATFGCLI
                     DFFDGKTT"
     gene            6570..7541
                     /locus_tag="VD788_15260"
     CDS             6570..7541
                     /locus_tag="VD788_15260"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV77671.1"
                     /translation="MSRSAVVFGGSGFIGGHLLDSLAARGYARLLSVDIRPPVRRNPA
                     VEYLRCDLRQPIGDRVAGSFDEAYNLAAIHTTPGHPDHEYYDANVLGALHVVEFCRER
                     RIERVCFTSSISVYGPGEDAKTEESPLAPVSAYGKSKLMAERVHRLWAVAEPGRRLVI
                     ARLAVIFGPGEGGNFTRLAKALASGFFLYPGRRDTIKGCAHVDEVVRSFEFGLARSEP
                     VYLYNGCYPRPYTLQEVCDAFHEVGGLPRPRGTIPFWTMTAAVVPFEALNAIGLRNPI
                     CRARVDKLVHSTHISPRRLMQDGFVFETDLTEGLRRWKRARPDGAFV"
     gene            7551..9059
                     /locus_tag="VD788_15265"
     CDS             7551..9059
                     /locus_tag="VD788_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HXV77672.1"
                     /translation="MVTADDSSSKSGGIAPEATGRQLFRGSAWMISLRWTMRGIGLGS
                     TLILARLLTPEDFGLVAMASVGVGLLEVFTHAGVDLALIRNPSVTREHFDSAWTINIC
                     MGLALAAVLLGVAPLAAYYFGEPRIIVIIRVLAIRAVLGGFENIGVVTFRRDLDFATE
                     FRFEVTKKALNFVTTVALALILRNYWALVIGVVAAQAMAVLVSFLVHPFRPRFALSKV
                     AEIWTFSGWLILFRAGRYLNGRTDRLILGAIAGAKPTGSYHVAAELATAPTDELVFPM
                     ARGLFPVFSKMTTEPTLLANSYKNVFSVVSLLCIAAGFGMAAAAEEIVVVVLGEQWLS
                     AIPLVRTLALFGAVSGITGSFETLLTVMGRVRALAAITWLSFALQVPVLWAAGARWGP
                     EGVAIGRLGVALVVGPLVALVALRRFPLGVIPLIGTLSHRVLAGGLMVVAVLAPPFRS
                     IDVAVLRLAVEVGAGAVTYLAATLLFWKLRGSPPGPERGMLDWLGSKLGRGG"
     gene            complement(9071..9652)
                     /locus_tag="VD788_15270"
     CDS             complement(9071..9652)
                     /locus_tag="VD788_15270"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXV77673.1"
                     /translation="MIRPIPVAACLALLLCSAHPTLSQDATEEESAGDPSYVIGVEDV
                     LSVVTWGEPELSMSVKVRPDGRITLPLVNEVRVAGATPGEVRERIISVMGQYIRDPNV
                     TVIVEEINNFRVFFLGEINTQGPLQFYRPTRLLQAISTAGGLTEFSSKKITILREEFG
                     VEKRIEVDYKKLWSGDPGQENVYVKPGDTILVK"
     gene            9863..>11179
                     /locus_tag="VD788_15275"
     CDS             9863..>11179
                     /locus_tag="VD788_15275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03013 family XrtA/PEP-CTERM system
                     glycosyltransferase"
                     /protein_id="HXV77674.1"
                     /translation="MAAPVRTIRPRVLFCLALEAGLVFGVLAGLAQLNLRLTHSPAVA
                     DLRLTVLLTGGLFLGVLLLTQRAALGAASPLARELVVFSAISVVAGALVFASLAFVSA
                     ELTWLPALLAIQGAVAVPGAVSAWRWVSVRFDLLDLRREKLLLVGDGETAKDLCRWIA
                     SKHAREFSVIGFASENSSRVGSLLAMGSRVQTDYVSLPRFVPGRADRVIVALDEKRGR
                     LPVRELMELRLLGVEIEDSTSFLERVSGKISVESMLPSWLIFSEGFRISPFRTFVKRA
                     SDVFHATLLLVLTTPVMLLTALLIKIDSRGPILYRQARMGRFGREFNVLKFRSMRQDA
                     EKASGPTWAKSGDPRITRIGRFIRKTRIDELPQLINILRGEMSFVGPRPERRFFVRQL
                     ENKIPYYGLRMTVRPGLTGWAQVEYGYGASDDDALEKLKYDLYYIKN"
BASE COUNT         1905 a         3695 c         3639 g         1940 t
ORIGIN      
        1 tgaccatcac ggatcccgat ggttacagtg aaacccagca gatcagtatg aagacacgtg
       61 ctgagcctcc tacggccggt atcgcgacgt ccacaccctc gacactatcg cagatccgtg
      121 ccgcgatctc ctcggcatcg cccggcagca cgatcgagat tcaacctggc gtctacacgg
      181 cgtcttcagc cgacggcgag gtcctcgaca tcagcaatct ccgaggcacc gccaccaatc
      241 caattacgat tcgatgcgcg agccgtgata caacgacgct cgatgcgaac aacaattggt
      301 acggcatcta tggcggcgga acaatcgagt acgtccatat cgagaactgt acgatcgcga
      361 attaccgcag atgggacaac gcggaaagtg acgagtcgat ggcgtgtgtt tatctgaacg
      421 cgcacacgaa gggggttgtt ttccgtagca accgctgcag cggtcaaggc aatggggcga
      481 tcattggacc gtacaaggcc aatttcggca actggatctc caacaatact tttgatgggc
      541 caatcgcttt caattccggg cagccttccg accacgtggg catcgagatc atgggccagg
      601 gccacgtcgt ccgcaacaac acgctcacca atatgggcga cgcactgaac ctcgggcgag
      661 ccgagaacgc caccaactgg cctccggatc aaccgaccca ccgggcaaac gagttctctc
      721 tgaatgagat cgacgttgct ggcgacgact gtatggaatt cgacggaggc gagtcgaata
      781 acctcgcttg gcagaatcgc tgcacgaacg tcgtgtcgtg cttttcatgg caaccgacga
      841 acggtgcaaa caacgtggca gcgcgcaacg tctgtctgcc gtggatgggt cggtacgcac
      901 tcaagccggg gaacgagacc tggggaccgg tagcctacaa caatctcttc gggttaccgt
      961 ggatcatgtt ccagccgaac gggtttgcgg ccgatatccg aaacaatctg tgggtgggga
     1021 acccggatcg cgcaaagcga tccgtcgcaa atatcttcgc gacatccctc aagctgtgtc
     1081 gggacagccg agtggagaac ggttcgacgg cggtcgacaa ctgcaacggc ccactagcgt
     1141 tctggaacga ctacaatgcc tacaactatg atgggccgta cgatctggca cctatcgacg
     1201 aaccgaattt tgcggctgcc aaggctgcag cgccggagta cgaagtcggc gtcagacaaa
     1261 tgcagcatga cgtcctgatc tcgcccggaa acgcgagcga tattttcgcc gaccagaact
     1321 actcgttccc agataccgcg tcctgcgggt gttctgggtc gccgtcagta tgcgactgtg
     1381 acgcctggac taataatgac gttcagccac ggatcgacgc gagtcccaag gccggggcgc
     1441 agcatgtcga cgccggaacg gaccttggcg ccttcaccgc tggcttcagc gggaacgctc
     1501 cggacatcgg cccgatagag ctagggcaag ccgtaccggc gtacggagcg gactggtcaa
     1561 gtgctgatcc gccggatccg ccttcccctc cgacttctct cttcgccgtc gcagtcagtt
     1621 ccacccgaat cgacctctcc tggtcgacgc cgcaaggcgg cggaccggtc gcatactaca
     1681 agctgtaccg tgacgatgta ctcgttggcc agaccggggc gatttccttc tcggacgtgg
     1741 gcttgagtga ggcgacgtcg tactcttact acgtgacttc ggtgggcggc ggcctggagt
     1801 ccgacccgag ctctaccgtg gtcgcgacga ccttggtcga taccaccgca cccgccatcc
     1861 aatcggttcg cgcagacggc gacccgactc gggtgttcgt cgtctttacg gagccggtcg
     1921 agcagtcgag cgcgtcgaat agctcgaact acgcgatcga caacggcatc gcgatcacct
     1981 cggccgcgtt gagccccgac ctcgtcacgg tcgtgttgtc cacgaccgtt cacatcgaga
     2041 acgtgatgta cacgctgacc gtcgatgacg tgagggaccg ctcggccaac gcgaacccga
     2101 tcgcgccagg aaccacggct tcgtacctct acagcgcggt gctcgaactc tccaacctga
     2161 acgtactctc gggcaagtcg taccaggtgg tggagaatct ggccaacggc gacctggtct
     2221 atatcgatcg atcgttcacc tacagccagg taccgaccta cttgctgggg tcgacctaca
     2281 tcagaaccgc gaacgacgac aagctcagcg acggcaacgt cgatttcctc agcttcgacg
     2341 tcaatcgcga cgtcactgtt ttcgtcgcgc acgacgatcg gtacacgatc aagcccggtt
     2401 ggttggccga tttcgtcgac accggtgaca acgtggtctc gacctccacg ttcagcctgt
     2461 tccggcgaga ctatcctcgg ggtacgatcg tcctcggggg caacgtgcac cccaacgagg
     2521 cagaggacaa cgcgatgtac acggtaatct ttgtcggatc gacgttcgat ccgggcaatc
     2581 ccccgcccga gccgtcgaat ccacgggtcc actgacacgg ggcgccggcc ggactacgga
     2641 ctaccggcgc tggccggcgc gcgatggact gagagaggag ggggcgggtc gggttccggg
     2701 gttgacctcg cgaccctcga ctgacggccc gccggcgtgg acggccgccg tcccctcacg
     2761 gacgacggcc tgcgtcaaca ccacgagcgc gatcaggttg tagaacaggt cgaagtaggc
     2821 cagcccgagg aacgcgccgc cgaccgcgta gccgacgata ctgacctgca gcatccgtga
     2881 caggtcgtga gcccagatca agttcgggtt gttcgccgcg gccgcggccg ttctggtcgc
     2941 cgctcgccag ctggcgaagg ccagggcgac gaagatcgcg aggccgccgt aaccatgctc
     3001 tgcgaggacc tcgaagtaga tgctgtgcgc gtcgtagacg atcgtggggt cgggggcgta
     3061 cttggcgaac atcggtgcct gaaacgtgcc gtatccgcca ccgaagaccg gattgtcgtt
     3121 ggccaggttg attgcgaagt tccaggagtt gattcgcgac atggccgatg cgtcctgctc
     3181 gtaggtcttg atcgtgccca tccgctcgaa ccagcgttcc ggcatcaggc ccagcgcgag
     3241 cgggatcaac accgccccca cgaccagcag tcgcaaacgg tggcgactct tgagcgcgag
     3301 gacgatcccc atcgcactgg cggcgaggaa gccgccgcgc gaataggtcc cgatgatggc
     3361 gaccaccgtc aggaccatca cgccgagcaa gccgagacgt atccaatggg ttctggtcga
     3421 gagctgcaga taccgcatca gcggtaatgt catcgtcaag gccaagccga gttcgttgtt
     3481 gcccgcgatg aaaccgccgt cgggccccca aacacggtac tgcccgcccg tgaccagcgt
     3541 catcagacca ccacgaacac cgaagtaccc gagcgacaga acgatcaccc agacggtcgc
     3601 atcgatccga ggccgcgacc acatgatcag catgatgcag agcgtgatga gctggatctt
     3661 gagcgtcttg ttcagcgcga cttcggcgat cgtcgggtaa aaggccagcc acgtcgtgat
     3721 gcactgccac ccgacccaca gaaaccacaa gccgacgacc ggcgaccgcg gcaatcgctt
     3781 tcgctcaccg gatatcaaga gccccgcgat cagggcgacg gcgacgaagg cggcgaatcg
     3841 catcccgcgg gccaatccgt gcgccagcgt ctgcggattc atgtagctca gccagcacca
     3901 gaggatgacg ccgatctgcg gtcggagcag gacgatcggt agcgccccga agaccacgac
     3961 cacaaggaga atgtctctca tcgggagagc cggctaccga ggcatcacga cgcggccacc
     4021 acgatcctcg aactgcgcac gcaacgacct ccgatccggt caatatatgc cttctagcat
     4081 cgaatactat cgtgaagtcg agatgcgggc cgcccgcgct ccggtcgcag cggcccggtt
     4141 gactctcccg atcgcgtcag ctaacgtttg gcttcctcga aattccgagg gttcggaagg
     4201 tcgtcggtgc tcggccatcc gagcttcgcc atgacgccgc gtcgctccgg cctcggtgat
     4261 cgcataaaga acgggtctcg cgcggaggat ctcttggtcg gctcggagtc acggctcggt
     4321 gcggcggctt gcgtcatccg cgagctggac gagccggccg cgaccggctg ggatcggttc
     4381 gtcctcgacc atccggcggg gacgttcttc caccgcgccg gctggaagca ggtgatcgag
     4441 cgggcgatgg gtcaccgctg ccgttacgcc tacgccgagc gggacggccg aatcgtcggt
     4501 gtcctgccgc tggcccacgt caagacggcg ctgttcggaa accgcctcgt gtcgaccccg
     4561 ttctgcgtct acggcgggcc gctcgcgacg gacgagaccg tgcgacgggc gctcaccgag
     4621 catgcgatcc gcatccagga cgagctgcaa gtcgactggc tcgagttccg cagccgcgag
     4681 cggacacagc cgggatggac ctcccaaggc gacctctact cgaccttccg caaggagatc
     4741 ccgacgggcg agcaggagat cctcaagtcg atcccgcgca aggcgcgggc ggtggtccgc
     4801 aaggcgatcg acgacgatgc gctcgaagag cgaaccgacg agactcccga ccggttccat
     4861 cacgtctatt ccgtcagcgt gcggaacctc ggttcgccgg ttttctccag gaagtactgc
     4921 cgctggctca agcaggcgtt cggcgacgac tgcgagttct tcgtgctcgc gacgcgcggc
     4981 gggctgcccg tcgccggcgt gctcagtttc tatttccgcg acgagatcct gccgtactac
     5041 ggcggcggga ccgaggcggc gcgggagtac ggcgcgcacg cgtacatgta ctggcgattg
     5101 atggtccgcg cggcggcacg cggcgttcgg gtcttcgact tcgggcgcag caaacgcggc
     5161 accggggcct actcgttcaa gaccaacttc ggcttctcgc cggacacact gctctacgag
     5221 taccggatga aggaaggcgt cagcctgccc gacgcgacca cgctcaatcc caagtaccgg
     5281 agcttgatcg cgacgtggaa gcgcttgccg cttccgatcg cgaacgccat cggccccttc
     5341 ctcgtgcgaa ggctggggta gccgaatcac gtggaacatc tcgtcttcat ctcgcagcgc
     5401 atcccgtacc cgccgaacaa gggtgacaag atccgttcgt ggaatgcgtt gatccgattg
     5461 gcggaacgct acagggtcca cgtcggcagc ttcgtcgacg acccggagga ttggggtcac
     5521 gccgacaaga tcggcgagat cagcgcgtcg tacaggctgc tgcccctgcg ccggacggcc
     5581 aagctgtgga gcctgcgcgg cctggcgacc ggcgaggcgc tctcgctccc ctattttcgc
     5641 gaccggcgac tggtgcgctg ggtcgagtcg acgcgcgaac ggtacgcgcc gcgggcggcg
     5701 ttcaccttct cctcgggtgt cgccggttac gtgatggggc cgcgctgggc cggcgtgcgc
     5761 cgcgtccacg acatggccga cgtcgactcc gacaagtggc tgcagtacgc gaagaagaag
     5821 tccggcgtcg cgcggatgat ctattcgcgg gaaggtcgaa agctgctggc gttcgaacgc
     5881 cgggtggcgg cggagtacga cgcgacgctg ttcgtctcgg aggacgaggc ggcgatgttc
     5941 cgcagattgg cccccgagtc ggccgggcgg atccacgcgc tgcacaacgg cgtcgacgcc
     6001 gactacttcg accccgccgt cggacacgac gacccgtttc ccgccgagat cacgccgatc
     6061 gtgatgaccg gcacgatgga ctactggccg aacgtcgacg ccgcgcagtg gttcgccgac
     6121 gaggcgctgg acgaggttcg gcggatcatc ccgaacgcgg cgttctacgt ggtcggcgcc
     6181 aacccgaccc ggacgcttcg cgcgctcggc gagcggccgc acgtgacggt gaccggtcgc
     6241 gtcccggacg tccggccgta cctggcccac gcccgtttcg tcgtggcacc gctccgcatc
     6301 gcgcgcggag tccagaacaa ggtgctggaa gggatggcga tggcgcgccc ggtgctggcc
     6361 agcgcgatgg ccctcgaagg gatccgcgcc gagatcggat cggaagtgct gctggccgac
     6421 agcgccgccg attttgcccg ccacgccgcg tcggtcgacg aggcgaccgg cgccgcgatc
     6481 ggcgggcgcg cgcgcgcgcg cgtgctcgcg agctactcgt ggaacgcgac gttcgggtgc
     6541 ttgatcgact tcttcgacgg gaagacgacg tgagtcgatc ggcggtcgtc ttcggcggga
     6601 gcggcttcat cggcggccac ctgctcgatt cgctcgccgc gcgcgggtac gcgcggctgt
     6661 tgtcggtcga catccgcccg ccggtccggc gcaaccccgc cgtcgagtac ctgcgttgcg
     6721 acctgcgcca gccgatcggc gaccgcgtgg ccggcagctt cgacgaagcc tacaacctgg
     6781 cggcgatcca cacgacaccc ggccacccgg accacgagta ctacgacgcg aacgtgctcg
     6841 gcgcgctgca cgtcgtcgag ttctgccgcg agcgccggat cgagcgggtc tgctttacca
     6901 gctcgatctc ggtctacggc ccgggcgagg acgcgaagac cgaagagtcg ccgctcgcac
     6961 cggtctcggc ctatggaaaa tcgaagctga tggccgagcg cgtccaccgt ctgtgggcgg
     7021 tggccgagcc gggaagacgc ctcgtgatcg ctcgactggc ggtaatcttc ggcccgggtg
     7081 agggcggcaa cttcacccgt ctggccaagg cgctcgccag cggtttcttc ctctacccgg
     7141 gacgccgcga cacgatcaag ggctgcgccc acgtcgacga ggtcgtgcgc agcttcgagt
     7201 tcggcctcgc gcggagcgag ccggtctacc tctacaacgg ctgttatccc agaccgtaca
     7261 cgctgcaaga ggtctgcgac gcgttccacg aggtcggcgg tctgccgcga ccgaggggaa
     7321 cgatcccgtt ctggacgatg acggcggccg tcgttccctt cgaagcactc aacgcgatcg
     7381 gcctgcgtaa cccgatctgc cgcgcccgtg tcgacaagct ggtgcactcg acccacatct
     7441 ccccccggcg cctgatgcag gacgggttcg tcttcgagac ggatctgacc gagggcctgc
     7501 gccgctggaa gcgcgccagg ccggacgggg cgttcgtctg aacgagggct atggtcaccg
     7561 ctgacgactc gtcttcgaaa tccggcggga tcgcccccga ggcgaccggc cggcagctgt
     7621 tccgcggctc ggcctggatg atctcgctgc gctggacgat gcggggcatc ggcctcggaa
     7681 gcacgctgat cctggcgcgg ctgctgacgc cggaggattt cgggctcgtc gcgatggcgt
     7741 cggtcggcgt cgggctgctc gaggtgttca cccacgccgg agtcgacctc gcgctgatcc
     7801 gcaacccgtc ggtgacgcgc gagcacttcg actcggcgtg gacgatcaac atctgcatgg
     7861 gactcgcgct ggcggcggtg ctgctgggcg tggcaccgct cgcggcgtac tacttcggcg
     7921 agccgcggat catcgtcatc atccgcgtgc tggcgatccg cgccgtgctc ggcggcttcg
     7981 agaacatcgg cgtcgtcacc ttccggcgcg acctcgactt cgcgaccgag ttccgcttcg
     8041 aggtcacgaa gaaagcgctc aacttcgtca cgacggtcgc gttggcactg atcctgagga
     8101 actactgggc gctcgtcatc ggcgtcgtcg cggcccaggc gatggcggtg ctcgtcagct
     8161 tcctggtcca tcccttccgg ccgcgattcg cgctgtcgaa ggtggccgag atctggacct
     8221 tctccggctg gttgatcctg tttcgcgccg gacgctatct gaacggccgg accgatcgcc
     8281 tgatcctcgg cgcgatcgcc ggcgcgaagc cgaccggaag ctaccacgtc gcggccgagc
     8341 tggcgacggc gccgacggac gagctggtct tcccgatggc gcgcgggctg ttcccggtct
     8401 tctccaagat gaccacggag ccgacactgc tcgcgaacag ctacaagaac gtgttcagcg
     8461 tcgtctccct gctctgtatc gcggcgggct tcggcatggc ggccgccgcc gaggagatcg
     8521 tcgtggtcgt cctgggcgag cagtggctga gcgcgatccc cctggtgcgg acgctggcac
     8581 tgttcggtgc ggtctccggc atcaccgggt cgttcgagac gctgctgacg gtgatgggcc
     8641 gggtgcgggc gctggccgcc atcacctggc tcagcttcgc gctccaggtg ccggtgctgt
     8701 gggcggccgg ggcccgctgg ggccccgagg gcgtcgccat cggccgcctg ggcgtcgcgc
     8761 tcgtcgtcgg accgctcgtc gcgctcgtgg ccctcaggcg gtttccgctg ggcgtcatcc
     8821 cgttgatcgg cacgttgagc caccgcgtgc tggcgggcgg tttgatggtg gtggcggtgc
     8881 tcgccccgcc gtttcgatcg atcgacgtcg ccgtcctgcg gctcgcggtg gaggtcggtg
     8941 ccggcgcggt tacatatttg gcagcgacgt tacttttctg gaagctacga gggagcccgc
     9001 ctgggcccga gcgcgggatg ctcgactggc tcggatcgaa actggggagg ggcgggtagg
     9061 cgctcgggag tcacttgacc aggatcgtgt cgcccggctt gacgtagacg ttctcctgac
     9121 ccggatcgcc cgaccagagc ttcttgtagt cgacctcgat gcgcttctcc acgccgaact
     9181 cctcgcgcag gatggtgatc ttcttgctcg agaactcggt cagcccaccg gccgtcgaga
     9241 tcgcctgcag cagccgcgtc ggccggtaga actgcagcgg tccctgggtg ttgatctccc
     9301 cgaggaagaa cacacggaag ttgttgatct cctccacgat gacggtcacg ttggggtcgc
     9361 ggatgtactg gcccatcacc gagatgatcc gctcgcgcac ttcgccgggc gtcgccccgg
     9421 cgacccggac ctcgttgacc agcggcagcg tgatccgccc gtccggtcgt accttgacgc
     9481 tcatcgacag ctcgggctcg ccccaggtga cgacggaaag cacgtcctcc acgccgatga
     9541 cgtacgacgg gtcgccggcc gactcctcct cggtcgcgtc ctgcgacagc gtcggatgcg
     9601 cggagcagag caggagcgcg agacaggccg cgacagggat gggtcgaatc atgcccaggg
     9661 tctcctcggt tgcgttcgtt gcaaaggata cccccggaga gacgattcgc cagagccgac
     9721 tgcgccgaaa atcgcccaga aacccgttga cagcggccgc ccggcctcgt atcgtgagac
     9781 caaatcccga tcgggcacgg tccgagcaac cagtggggcg tgggacacga cccgagaatg
     9841 ggtgcgggtg tagaggtaca agttggcagc gccggttcgc acgatacgcc cccgggttct
     9901 cttctgcctc gctctcgaag ccggcctcgt cttcggcgtc ttggccggcc tggcccagct
     9961 caacttgcgg ctgacgcatt ccccggcggt cgccgacctc cggctcaccg tgttgctgac
    10021 gggcggtctg ttcctcggcg tgctgctctt gacccagcgc gccgcgctcg gcgcggcgtc
    10081 gccgctcgca cgggagctcg tggtcttcag tgcgatctcg gtcgtcgccg gtgcgctggt
    10141 cttcgcctcg ctcgccttcg tttcggccga gctgacgtgg ctgccggcgc tcctcgcgat
    10201 ccagggcgcg gtcgcggtgc ccggcgccgt ctccgcctgg cgttgggttt cggtacggtt
    10261 cgacctgctc gacctgcgcc gcgagaagct gttgctcgtc ggagacggcg agaccgcgaa
    10321 ggacctctgc cgctggatcg cgagcaagca cgcccgcgag ttctcggtga tcgggttcgc
    10381 gtccgagaat tcgagccgcg tcggcagcct gctcgcgatg ggctcgcggg tccagaccga
    10441 ctacgtctcg ctgccgcgtt tcgtcccggg acgcgccgat cgcgtgatcg tcgcgctcga
    10501 cgaaaagcgg ggccggcttc cggtgcgcga gctgatggag ctccggctgc tcggcgtcga
    10561 gatcgaagat tcgacctcgt tcctcgagcg cgtcagcggg aagatctccg tcgagtcgat
    10621 gctgccgtct tggctgatct tctccgaggg ctttcgcatc tccccgttcc ggacgttcgt
    10681 caaacgcgcg agcgatgtat tccacgccac cctcctgctc gtattgacca ccccggtgat
    10741 gctgctgacc gcgctgctga tcaagatcga cagccgcggt ccgatcctct accggcaggc
    10801 gcgcatgggc cgcttcgggc gggagttcaa cgtgctcaag ttccgctcga tgcgtcagga
    10861 cgccgagaag gccagcggcc cgacctgggc caagagcggc gacccgcgga tcacccgcat
    10921 cggccggttc atccgcaaga cgcggatcga cgagctgccg cagctgatca acatcctccg
    10981 cggcgagatg agcttcgtcg gcccgcggcc cgagcgccgc ttcttcgtca ggcagctcga
    11041 gaacaagatc ccctactacg gcttgcgcat gacggttcgt cccgggctca ccggctgggc
    11101 ccaggtcgag tacggctacg gcgcgagcga cgacgacgcc ctcgagaagc tcaagtacga
    11161 cctgtactac atcaagaac
//
LOCUS       DASPGN010000525        10532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1384905, whole genome shotgun sequence.
ACCESSION   DASPGN010000525 DASPGN010000000
VERSION     DASPGN010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1384905"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1838)
                     /locus_tag="VD788_15280"
     CDS             complement(<1..1838)
                     /locus_tag="VD788_15280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXV77675.1"
                     /translation="MSNVMLVNVTHVEESRVAILDDGVLAAYEIETINRTNIKGNIYN
                     AVVESVHATLEAAFVKLAPDLKGFLPLDGVNFKLLPARGERGKTGRIGQHLHPGQRLM
                     AQVVREPFAGKPPTVSTYFSLPGRFLVLMPGVDSSGISRKIEDAQQRERLKSVVDELK
                     LPDGFGLIVRTAGIGQTKTELQRDLRYLLRLWESVQKGSKGGEFPGLVYREADLVIRT
                     IRDFMTPDISEVWIDSKDTYETALRFVRDVMPNRARTVKLYTGDRPLFNKFNLEEQIE
                     RIYKRRVPLPSGGEIVIDGTEALTAIDVNSARSKRQGDIEDNNLQTNLEAASELTRQL
                     RLRDLGGLIVVDFIDMAASRNRSKVEKAMRDAMRGDRAKYDLTRLSKLGLMEIARQRL
                     KGAKMASTYATCVACEGYGLVKNVETAALAALRKLQTRSARANIGLMRVALPHDVATW
                     LLNHKREELMRIEQRHSIRVEIVPSSKLLRHESEFEAVARERTDAVDEPPAREPRSEI
                     QPDTPRLAPPPRPPIEPGEEKIGPRPLPAPVAAVDQETRRAEPRAEVVSESADVDGQT
                     TTEPPPVAPTNGELRAKRRRRRRRRGRAGAPPPADGQHEPASPKDEP"
     gene            2133..3764
                     /locus_tag="VD788_15285"
     CDS             2133..3764
                     /locus_tag="VD788_15285"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXV77676.1"
                     /translation="MSRTLRASPLACAVLAVGLPWLAGCTNAPQARPVPAEPVRGGVL
                     RMVQEAPQTLDPWRSASVYDSLPISQIFDTLVAYDPSLNVVPRLAETWTISRDSREYT
                     FFLRPAVRFHNGDPLTANDVVYSISRQLMPNHAGISLAFSYLMTIDGAPEYSRGQTDH
                     VRGLEIVDARTVKFRLARPYPSFLELLAMENLAVVPERVVRQVGTFEFGRKPVGTGPF
                     SLADWDDDGITLAANRDHFHGAPYLDGVRIAFLRPDENDYGAARFLDSQLDVLEPPTE
                     SLDLLAVDDSVTLHRYQELSVSFLGLNTEVAPLDDRSVRRAIAHALNRQAMVDDSPSV
                     RREAVGILPPGLPGYSPEPKALDYNPERARRLLAQAGYPEGRGLPVIPLHTASQSKAA
                     IRLLRQVESDLGAVGIRLDVVPVSWSELGAHLDEGTAGAFLLAWIADLTDPDSFLRSM
                     FEPDGSGNYFGHLSDETNQLLEAGIREFDPVARARIYRRLEAQILQEAPLVPLYHSIG
                     VIAVRDHVEGFHPTPLGVAKVELERVWIRPADAPS"
     gene            3764..6085
                     /locus_tag="VD788_15290"
     CDS             3764..6085
                     /locus_tag="VD788_15290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV77677.1"
                     /translation="MPEIARRRRLEHSFAVTTTLLVMLVIGATVAVVHNRVQSALNRG
                     LSSRAVAISSSIGAVATPSLLAYNYPALQIAAERAVEDPALLYVVIHDKEGEIAGVAG
                     PTLATASIPVLGRVFREPTSRRITLVDGAGRTRPVLEAVVPVFVEGVDEPWGTVRVGL
                     GFTSVHTELRRLEAGLALSGFVLMLMALAASRFMARRITAPLRRLAEGTAALAGGDTS
                     FRIPVSGARELAELGHAFNTMMDRVQEKADESRAYENELAELNATLEQQVYERTRALE
                     ESEAQYRTLVEHSPDSILIIQGSRVCFVNPAFCTTFGLSENRALRADFRLDGIFDEPY
                     REIVDARITAWERGEPASPAQVKSRDAAGNLRELELRGSRIEYRGQPAAECLLVDMTE
                     AKRLRERLVDTERLRALGELSSGVAHDFNNLLGAILGRIQLLRARGFDEEIDRSMQVI
                     EKAAMDGRETVRRIQEFSRVRTDRPFRTVDLPEIIRDAVEITRTRWKNEAERRNVNIR
                     VKVTSEAVPPILGNAHELREVYTNLILNAVDAMPDGGDLVLRCRTAGDKVRSEVEDSG
                     IGMSEVARRHLFDPFFTTKGHSGTGLGMSVAYGIVTRHDGTIEVRSRPGEGTSFTLEF
                     PVCDRPVDVAGGDGAVTPDLVRPGRILVIDDEQPVAQVLEDALTGAGHTVETAGSGRE
                     GVERASAAEFDLVLTDLGMPDMSGWEVASEIRAHRPHVPVILVTGWGATLSREEVERS
                     GVSAVVHKPFEIQELIETTGAVLARCRDDERPR"
     gene            6167..7534
                     /locus_tag="VD788_15295"
     CDS             6167..7534
                     /locus_tag="VD788_15295"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HXV77678.1"
                     /translation="MPANRIVRIANGQGFWGDSVDAPVRLIEGGPLDYLTLDYLAEVT
                     MSIMQKLRRRDPDRGYATDFIDVVDRVLPAIRERGIRIVANAGGVNPAACRRALIELA
                     RSRRATGLKIGTVTGDDILDRLDELLDDGVPLANLDDGRPFDEIRDRVLSANVYIHSF
                     AVADALRQGADIVVSGRTTDPGLVLSPLIAEFGWRPEQWDLLAAGTIAGHIVECGAQC
                     TGGNFSRWWEVEGWERIGYPVLECSVDGTFVVTKHDDTGGMVTVDTVGEQLLYELGAP
                     ERYVTPDCTVDFTSVRLDQQGPDRVRVSGVRGRPATDTYKVSIAYLEGYKAVGSLTVS
                     GPDALAKARVCADALWGRLRHAGFDYDKRITEYVGVDACHGGIAPRRPFVNEVVLRVG
                     VQDADRARVDRFGKELAPLVTSGPPGVTGFAGGRPKATEVLGYWPSLIPKDRVVPEIS
                     VEVAS"
     gene            7531..7923
                     /locus_tag="VD788_15300"
     CDS             7531..7923
                     /locus_tag="VD788_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77679.1"
                     /translation="MKRLPLYALAHARSGDKGDGSNVGVLAYDDRGFALLREWLTPER
                     VRRHFTGIVHGEVERYELPNLRALNFILHDSLGGSGSSSLKTDAQGKTHAMALLRIEV
                     EVPDDYRPPRVGRAGALAPGGELTGDGD"
     gene            7930..8703
                     /locus_tag="VD788_15305"
     CDS             7930..8703
                     /locus_tag="VD788_15305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXV77680.1"
                     /translation="MRLVQIESATSGVRTLVLDRPPVNALGHELVDELTVALGRLSSD
                     DQARCLIVRSAGAHFCAGADLKERATMSVEEVREFVPRLAGVCSALAAIPYPTIAELR
                     GAAAGGGCELALACDLRVMAEDGKIGLRETALAIIPGAGGTQRLPRLIGPMKAKRWIF
                     SAAMHDAAEALADGVVQRVVPQHRLNREVQELAEAIAACGPLANRLAKRAIDRGAELP
                     IDRGLQLEWECYQETLQTEDRVEALRAFAEKRPPRFGGK"
     gene            8712..9692
                     /gene="dusB"
                     /locus_tag="VD788_15310"
     CDS             8712..9692
                     /gene="dusB"
                     /locus_tag="VD788_15310"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HXV77681.1"
                     /translation="MLRYGAVDIETPLVLAPMAGVTDRQFRLVLRRVGGVGLVTMEFI
                     SSEALTRGNRRTIALMHYSEEERPLSIQIYGSSPERMAEAARVVEAIGADVCDVNMGC
                     PANKVLKGCAGCALMGDLDLARRIIAAVRRAIRIPLTVKFRAGVRDDALNYLELGRIC
                     QQEGVDGVALHPRTAKQMYRGRADWGRIARLKQELSIPVVGNGDVATPDDVRRMFDET
                     GCDGVMIGRASMRNPWIYRQALALMRGDPPVEPTPDDRRDLILAHFRLLMRQESDVDF
                     ALHKLRTFTGWYTHGLPDGKQLRVRINSLSTPGDFLRAVEEFFDGTAAAA"
     gene            9728..>10532
                     /locus_tag="VD788_15315"
     CDS             9728..>10532
                     /locus_tag="VD788_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HXV77682.1"
                     /translation="MNLSEIQNALERAGFDGWLFFDFHHRDPMAYKILGLDAGGQTTR
                     RWFYFVPANGQPVKLSHKVEPTKLKRLPGEQRYYLPYTQLHDELARIVGGRRKIAMQY
                     SPMNNIPYVSVADAGTIELVRSFGVEVVSSADLVQQFEAVTGDEGFHSHREAGEKVIA
                     IKDEAFERMDRALRDRNRITEYDVRQFILARFDEEGLTTVGVPIVGFNDHPADPHFEP
                     TADNAYTLHDGDTILLDLWARRRDPPGVFYDVTWCGFAGATAPALYEEIW"
BASE COUNT         1572 a         3567 c         3681 g         1712 t
ORIGIN      
        1 ggttcatctt tcggcgaggc cggctcgtgc tgcccgtcgg cgggcggcgg cgcgcccgcc
       61 cgcccgcgcc gccggcgtct ccgccggcgc ttggctcgca gctcgccatt cgttggtgcg
      121 accggcggtg gctcggtggt cgtctggccg tcgacatcgg ccgactccga caccacctcg
      181 gcgcgtggct ccgctcttct cgtttcctga tcgaccgcgg cgaccggggc cggaagcgga
      241 cgaggtccga tcttctcctc gcccggctcg atcggcggtc gcggcggcgg ggccagacgc
      301 ggcgtgtccg gctggatctc gctgcgcggc tcgcgcgccg gtggctcgtc gaccgcgtcc
      361 gtccgctccc gggcgacggc ctcgaactcg ctctcgtggc gcagcagctt ggacgacggc
      421 acgatctcga cgcggatcga gtgacgctgc tcgatgcgca tcagctcctc acgtttgtgg
      481 ttcagcagcc acgtcgccac gtcgtgcggg agggcgaccc gcatcaggcc gatgttggcc
      541 cgagcgctgc gagtctgcag ctttcgtagc gccgctagcg ccgcggtttc gacgttcttg
      601 accagcccgt atccctcgca cgcgacgcag gtcgcgtagg tcgaggccat cttcgcgccc
      661 ttgaggcgct gccgcgcgat ttccatcaag ccgagcttgg acaggcgtgt caggtcgtac
      721 ttcgcccgat cgccgcgcat cgcgtcgcgc atcgctttct cgaccttgga tcggttgcgg
      781 ctggccgcca tgtcgatgaa gtcgacgacg atcaggccgc cgaggtcccg cagtctcagc
      841 tggcgcgtca gctccgatgc ggcctcgagg ttcgtctgca ggttgttgtc ctcgatgtcg
      901 ccctgacgct tggagcgcgc gctgttgacg tcgattgcgg tcagcgcctc ggtgccgtcg
      961 atcacgatct caccgcccga gggcagcggg acccggcgtt tgtagatgcg ctcgatctgc
     1021 tcttcgagat tgaacttgtt gaacaacggg cggtcaccgg tgtagagctt gaccgtccgc
     1081 gcgcggttcg gcatcacgtc gcggacgaag cgcagggcgg tctcgtaggt gtccttgctg
     1141 tcgatccaca cctcgctgat gtccggcgtc atgaagtcgc ggatcgtacg gatgaccagg
     1201 tcggcctcgc ggtagaccag gccggggaac tcaccaccct tcgagccctt ctgcaccgac
     1261 tcccacagtc gcagtaggta acgcagatcg cgctgcagct ccgtcttggt ctgaccgatg
     1321 ccggcggtgc gaacgatgag accgaagcca tcgggcagct tcagctcatc gaccacgctc
     1381 ttcagtcgct cgcgctgctg ggcgtcttcg atcttgcgcg agatcccgga cgaatccacc
     1441 ccgggcatca gcacgaggaa tcgcccgggg agcgagaagt aggtgctgac cgtcggtggt
     1501 ttgccggcga acggctcgcg cacgacctgc gccatcagcc gctgtcccgg atgcaggtgc
     1561 tggccgatgc gacccgtctt tccgcgctcg ccgcgagcgg gcagcagctt gaagttgacg
     1621 ccgtcgagcg ggaggaagcc cttgaggtcg ggtgcgagtt tgacgaaggc cgcctcgagc
     1681 gtcgcgtgga ccgactcgac gaccgcgttg tagatgttgc ctttgatgtt ggtgcgattg
     1741 atcgtttcga tttcgtaagc ggcgagaacg ccgtcgtcga gaatcgcgac tctcgactcc
     1801 tcgacgtggg tcacgttgac cagcatcacg ttcgacatgg ggcatgtctc tcccgggtcg
     1861 gggaccctgt tcggaatctc agtgctcgtg ctgcctttta tcgcgcgtag ctcccgggcc
     1921 ctgccaccgg tcgcgtccga tttcgcgctc cggagggggc cacacgggtt cccgccacgc
     1981 gggacctctt tctggcgggt cccgcggtat ctctggcgtg ggccgaaccg caccgactat
     2041 agcacagggc ccgtttgctt gactctgtgg ccgccgatcg cgaagtttgt ctcgggggtc
     2101 cggcacggtt ccggtttcgt tccgaggggt tgatgtcacg gaccttgcgc gcttcgccgt
     2161 tggcctgtgc cgtgctcgcc gtcggcctgc cgtggctcgc cggttgcacg aacgcgccgc
     2221 aggcgcgtcc cgtaccggcc gagccggtcc gcggcggcgt gctgcgcatg gtgcaggagg
     2281 caccgcagac gctcgacccg tggcgctcgg cctcggtcta cgattcgttg ccgatcagcc
     2341 agatcttcga cacgctcgtc gcgtacgatc cgtcgttgaa cgtggtgcca cggctggccg
     2401 agacctggac catctcgcgc gacagccgcg agtacacctt cttcctgcgt ccggccgtgc
     2461 gcttccacaa cggcgacccg ctcaccgcga acgacgtcgt ctacagcatc tcgcgccagc
     2521 tgatgccgaa tcacgcggga atcagcctgg ccttctcgta tctgatgacg atcgacggcg
     2581 caccggagta ctcccgcggc caaacggacc acgtgcgcgg gctggagatc gtcgacgccc
     2641 gcacggtcaa gttccgcctg gcgcgcccct atccgtcgtt cctcgagctg ctcgcgatgg
     2701 aaaacctggc cgtcgtgccc gagcgggtcg ttcgccaggt ggggacgttc gagttcgggc
     2761 gcaagccggt cggcaccggc ccgttcagcc tggccgactg ggacgacgac gggatcacgc
     2821 tcgcggccaa ccgcgaccac ttccacgggg cgccgtatct cgacggcgtc cgcatcgcgt
     2881 tcctccggcc cgacgagaac gactacggtg ccgcgcggtt tctcgactcg cagctcgacg
     2941 tgctggagcc gcccaccgaa agcctcgatc tcctcgcagt cgatgacagc gtgaccttgc
     3001 accgctatca ggagctgagc gtctcgtttc tcggcctcaa caccgaggtc gcgccgctcg
     3061 acgatcgttc ggtgcgccgg gcgatcgccc acgcgttgaa ccgccaggcg atggtcgacg
     3121 attcaccgtc ggtccgccgc gaggcggtcg gcatcctgcc tcccggtctg cccggttact
     3181 cgcccgaacc caaggcgctc gactacaacc cggagcgcgc gcggcggttg ctggcgcagg
     3241 ccggctaccc cgagggccgt ggtctacccg tgatcccgct gcacaccgcg tcacagagca
     3301 aggcggcgat ccgcctgctg cggcaggtcg aatcggacct cggcgcggtc gggatccgac
     3361 tcgacgtcgt gccggtgagc tggtccgagc tcggtgcgca cctcgacgag ggtaccgccg
     3421 gcgcgttcct cttggcctgg atcgccgacc tgaccgaccc cgactcgttc ctgcgctcga
     3481 tgttcgagcc cgacggctcc ggcaactact tcggccacct cagcgacgag accaaccagc
     3541 tgctcgaggc cgggatccgc gagttcgatc ccgtcgcgcg ggcgcggatc taccggcgcc
     3601 tcgaggcgca gatcctgcaa gaggctccac tcgtcccgtt gtaccactcg atcggcgtga
     3661 tcgccgtgcg ggaccacgtc gagggattcc acccgacgcc gctcggcgtg gcgaaggtcg
     3721 agctcgagcg ggtctggatc cggccggcgg atgcgccgtc gtgatgccgg agatcgcgcg
     3781 gcgacggcga ctcgaacaca gcttcgccgt caccaccacg ctgctggtga tgctcgtgat
     3841 cggcgcgacc gtcgccgtcg tgcacaatcg cgtgcaatcc gcgctgaacc gcgggctgag
     3901 ctctcgcgcg gtcgccatct cgagcagcat cggcgcggtc gccacgccgt cgctgttggc
     3961 ctacaactac ccggcgttgc agatcgcggc cgagcgcgcg gtcgaggatc cggcgctgct
     4021 gtacgtcgtg atccacgaca aggagggtga aatcgccggc gtcgcggggc cgacgctcgc
     4081 caccgcgtcg attcccgttc tcggccgagt gttccgcgag ccgaccagcc gccggatcac
     4141 gctggtcgac ggcgccggcc gcacccgtcc ggtgctcgag gcggtcgtgc cggtcttcgt
     4201 cgagggggtc gacgagccgt ggggtaccgt ccgcgtcgga ctgggcttca cctcggtgca
     4261 caccgagctg cgccggctgg aggccggtct ggcgctttcg ggcttcgtgc tgatgttgat
     4321 ggcgctggcg gccagccggt tcatggcgcg gcggatcacg gccccgctgc gccggctggc
     4381 ggagggcacg gccgcgctgg ccggcggcga tacctcgttc cgtattccgg tgtcgggtgc
     4441 gcgggagctg gccgagctgg ggcacgcgtt caacacgatg atggatcggg tccaggaaaa
     4501 ggccgacgag tcgcgcgcgt acgagaacga gctggccgag ctcaacgcga cgctcgagca
     4561 acaggtctac gagcggacgc gagcgctcga ggagtcggaa gcccagtacc gcacgctggt
     4621 cgagcattcg cccgactcga tcctgatcat ccagggcagc cgggtctgct tcgtcaatcc
     4681 ggcgttctgc acgaccttcg gcctttcgga gaatcgggcg ctacgcgccg acttccgcct
     4741 cgacggcatc ttcgacgagc cgtatcgcga gatcgtcgat gcacggatca ccgcctggga
     4801 gcgcggcgag ccggcctcgc cggcccaggt caagtcgcgc gacgcggcag gcaatctgcg
     4861 cgagctggaa ctgcgcggca gccggatcga gtaccgcggt caacccgcgg cggagtgcct
     4921 gctggtcgac atgaccgaag ccaagcgtct gcgcgaacga ctggtcgaca ccgagcggct
     4981 gcgcgcgctc ggcgagttgt cgagcggcgt cgcgcacgac ttcaacaacc tgctcggtgc
     5041 gatcctcggt cggatccagc tgctgcgcgc ccgtggcttc gacgaggaga tcgaccgcag
     5101 catgcaggtg atcgagaagg cggcgatgga cgggcgcgag acggtgcgtc gcatccagga
     5161 gttctcgcgc gtgcgcaccg atcgaccgtt ccgcacggtc gatctgccgg agatcatccg
     5221 cgacgcggtc gagatcaccc gcacccgctg gaagaacgaa gccgagcgac gcaacgtcaa
     5281 tatccgcgtc aaggtgacga gcgaggccgt gccgccgatc ctgggcaacg cacacgagct
     5341 gcgcgaggtc tacaccaacc tgatcctcaa cgcggtcgac gcgatgcccg acggcggcga
     5401 cctcgttctg cgctgccgca ccgccggcga caaggtgcgc agcgaggtgg aggacagcgg
     5461 gatcggcatg tcggaggtgg cgcgccgcca cttgttcgac ccgttcttca cgaccaaggg
     5521 tcactccgga accgggctcg gcatgagcgt ggcgtacggc atcgtcaccc ggcacgacgg
     5581 cacgatcgag gttcgcagcc ggccgggcga gggaaccagt ttcacgctgg aattccccgt
     5641 ctgcgaccgg ccggtcgacg tggcgggcgg cgacggcgcg gtcacgcccg atctggtgcg
     5701 ccccggtcgc atcctcgtca tcgacgacga gcagccggtc gcgcaagtac tcgaggacgc
     5761 gttgaccggc gccgggcaca cggtcgagac cgccggctcc ggtcgggaag gggtagagcg
     5821 cgcctccgcc gcggagttcg acctggtgct gaccgatctc ggcatgcccg acatgagcgg
     5881 ctgggaggtg gcgagcgaga tccgcgccca tcgcccgcac gtgccggtga tcctcgtcac
     5941 cggctggggc gcgacgctca gccgcgagga ggtcgaacga tccggtgtca gtgccgtggt
     6001 gcacaagccg ttcgagatcc aagagctgat cgagaccacc ggggccgtcc tggcccgctg
     6061 tcgcgacgac gagcgaccgc gctgatcggt cattccgcta taatcctccc cccgccagca
     6121 gcgacaccga cgcgccggaa gcgacgcgcg gggggagaat cgcgacatgc cggccaatcg
     6181 aatcgtgcgc atcgccaacg gccagggttt ctggggcgac tccgtcgatg caccggtgcg
     6241 gctgatcgag ggcggaccgc tcgactacct gacactggac tacctggccg aggtgacgat
     6301 gtcgatcatg cagaagctgc gacgccggga tccggaccgc ggatacgcca ccgacttcat
     6361 cgacgtcgtc gaccgcgtac tgccggcgat tcgcgaacgc gggatccgca tcgtggccaa
     6421 cgccggcggg gtcaaccccg cggcgtgccg gcgcgcgctg atcgagctcg cccgctctcg
     6481 acgggccacc gggttgaaga tcggcacggt caccggcgac gacatcctcg accggctcga
     6541 cgagctgttg gacgacgggg tcccgctggc caacctcgac gacggccggc cgttcgacga
     6601 gatccgcgat cgcgtgctgt cggccaacgt ctacatccac tcgttcgccg tcgccgacgc
     6661 gctcaggcaa ggcgcggaca tcgtcgtcag cgggaggacg accgacccgg gtctggtgct
     6721 cagcccgctg atcgccgagt tcggctggcg accggagcaa tgggacttgc tcgccgccgg
     6781 gacgatcgcc ggccacatcg tcgagtgcgg cgcgcagtgc accggcggga acttttcgcg
     6841 ctggtgggag gtcgaggggt gggagcggat cggctacccg gtgctcgaat gctcggtgga
     6901 cggcacgttc gtcgtcacca agcacgacga cacgggcggc atggtgacgg tcgacacggt
     6961 cggcgagcag ctgctctacg agctgggtgc gccggagcgc tacgtcacac cagactgcac
     7021 cgtcgacttc accagcgtgc ggctcgacca acagggcccc gaccgcgtgc gcgtttccgg
     7081 cgtgcggggt cgtcccgcga ccgacacgta caaggtgtcg atcgcctatc tcgagggtta
     7141 caaggcggtc gggagcctga cggtcagcgg cccggacgcg ctggccaagg cccgcgtttg
     7201 cgccgacgcg ttgtggggcc gcctgcgcca cgcgggtttc gactacgaca agcggatcac
     7261 cgaatacgtc ggcgtcgacg cctgccacgg cggaatcgcg ccgcgccgac cattcgtcaa
     7321 cgaggtcgtg ttgcgcgtcg gcgtgcagga cgcggaccgc gcgcgggtgg accgcttcgg
     7381 caaggagctc gcgccgctgg tgacgtccgg gcctccgggc gtgaccggct tcgccggagg
     7441 ccgtccgaaa gcgaccgagg tgctgggcta ctggccgtcg ttgatcccga aagaccgcgt
     7501 ggtgcccgag atcagcgtcg aggtcgcgtc gtgaagaggc tcccgttgta cgcgctggcc
     7561 cacgcgcggt cgggtgacaa gggcgatggg tcgaacgtcg gtgtgctggc ctacgacgac
     7621 cgcggcttcg cgttgctccg cgagtggctg acgccggaac gcgtcaggcg gcacttcacc
     7681 ggcatcgtgc atggcgaggt ggagcgctac gagctcccca acttgcgcgc gctcaacttc
     7741 atcctccacg attcgctggg cggcagcggg tcgagcagcc tgaagaccga cgcgcaaggc
     7801 aagacccacg cgatggccct gctgcggatc gaggtcgagg tgcctgacga ctaccggccg
     7861 cctcgtgtgg gccgcgccgg agcgctcgcc cccgggggcg agctgaccgg cgacggagac
     7921 tgaggcgtca tgcggctggt ccagatcgag agcgcgacga gcggcgtccg cacgctggtg
     7981 ctcgaccgcc cgccggtcaa cgcgttgggg cacgagctgg tcgacgagtt gacggtcgcg
     8041 ctcggcaggc tgagctcgga cgaccaagcg cggtgcctga tcgtgcgctc ggcgggcgcc
     8101 catttctgcg cgggtgccga tctgaaggag cgcgcgacga tgagcgtcga ggaggtgcgc
     8161 gagttcgtac cgcggctggc cggcgtctgc agcgcgctgg ccgcgattcc gtacccgacg
     8221 atcgccgagc tgcgtggggc cgcggccggc ggcggctgcg agctggcgct ggcctgcgac
     8281 ctgcgcgtga tggccgaaga cggcaagatc gggctgcgcg agaccgcgtt ggcgatcatc
     8341 cccggtgcgg gagggaccca acgtcttccg cggttgatcg ggccgatgaa ggccaagcgt
     8401 tggatcttct ccgctgcgat gcacgacgcg gccgaggccc tggccgacgg cgtggtccaa
     8461 cgggtcgttc cccagcaccg gctgaaccgc gaggtgcagg agctggccga ggcgatcgcg
     8521 gcgtgcggtc cgctggccaa ccggctggcc aagcgggcga tcgaccgcgg cgccgagctc
     8581 ccgatcgatc gggggctgca gctcgagtgg gagtgttacc aggagacgct gcaaaccgaa
     8641 gaccgggtcg aggcgttgcg tgcgttcgcg gaaaagcgcc caccccgctt cggcgggaag
     8701 tgagccgggc gatgctgcgt tacggagccg tcgacatcga gacgccgttg gtgctcgcac
     8761 cgatggccgg cgtgaccgat cgccagtttc gtctggtgct gcgccgcgtg ggcggcgtcg
     8821 gtctggtgac gatggagttc atctcgtccg aggcgctgac gcgcggcaat cgccgtacga
     8881 tcgcgctgat gcactactcc gaggaggaac ggccgctgtc gattcagatc tacggctcga
     8941 gtccggagcg gatggccgag gcggcgcgcg tcgtcgaggc gatcggtgcc gacgtctgcg
     9001 acgtcaacat gggctgtccg gccaacaagg tgctcaaggg ctgcgccggc tgcgcgttga
     9061 tgggcgatct cgacctcgcc cgtcggatca tcgccgccgt gcgtcgcgcg atccgcattc
     9121 cgctgaccgt caagttccgc gcgggcgtgc gcgacgacgc gctgaactac ctcgaactcg
     9181 gccgcatctg ccagcaagag ggggtcgacg gggtggcgct gcatccgcgg acggcaaagc
     9241 agatgtaccg cgggcgtgcc gactggggcc gcatcgcccg gctcaagcag gagctgtcga
     9301 tcccggtcgt cggcaacggc gacgtcgcga ccccggacga cgtgcggcgc atgttcgacg
     9361 agaccggctg cgacggcgtg atgatcggcc gcgcctcgat gcgcaacccg tggatctacc
     9421 ggcaagcgct ggcgctgatg cgcggcgacc cgccggtcga gccgacgccc gacgatcggc
     9481 gcgacttgat cctcgcgcac ttccgcctgt tgatgcggca ggaaagcgac gtcgacttcg
     9541 cgctgcacaa gctgcgcacc ttcaccggct ggtacaccca cggtctgccc gacggcaagc
     9601 agctcagggt gcgaatcaac tcgctctcga cgcccggcga cttcctcagg gcggtcgagg
     9661 agttcttcga cgggaccgcg gcggcggctt gaccggggat cggcacgatc gcccgtggag
     9721 tgatccgatg aacttgtccg agatccagaa cgccctcgag cgggcggggt tcgacggctg
     9781 gctgttcttc gatttccatc accgcgatcc gatggcctac aagatcctcg ggttggatgc
     9841 cggcgggcag accacccgcc gctggttcta cttcgttccg gccaacggcc aaccggtcaa
     9901 gctgtcgcac aaggtcgagc cgaccaagct caagcggctg cccggcgagc agcgctacta
     9961 cctgccctac acccagctgc acgatgagct ggcccgcatc gtcggcggca gacgcaagat
    10021 cgcgatgcag tattcgccga tgaacaacat cccgtacgtg tcggtcgccg acgcggggac
    10081 gatcgagctg gtgcgctcgt tcggagtcga ggtcgtgtcg tcggccgatc tggtccagca
    10141 gttcgaagcg gtgaccggcg acgagggctt tcacagccac cgcgaggccg gcgagaaggt
    10201 gatcgcgatc aaggacgagg ccttcgaaag gatggatcgg gcgctgcgcg accggaatcg
    10261 gatcaccgag tacgacgtcc gccagttcat cctcgcgcga ttcgacgagg aagggttgac
    10321 cacggtcggc gtgccgatcg tcggcttcaa cgaccacccg gccgacccgc atttcgagcc
    10381 gacggcggac aacgcctaca cgctgcacga cggcgacacg atcctgctcg acctgtgggc
    10441 ccgccggcgc gatccccccg gcgtgttcta cgacgtgacg tggtgcggat tcgccggcgc
    10501 gacggcgcca gcgctgtacg aggagatttg gc
//
LOCUS       DASPGN010000526         3176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_450112, whole genome shotgun sequence.
ACCESSION   DASPGN010000526 DASPGN010000000
VERSION     DASPGN010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450112"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VD788_15320"
     CDS             complement(<1..609)
                     /locus_tag="VD788_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77683.1"
                     /translation="MRRRPGRWLAIALAAACVSAATAARAQSDRTERDRPREIGLEER
                     AGRSLAQIDITVVGPPEIVRSLTADDFKIRVQLERVDEFELDRLCGAATAAGTAGAVL
                     PSAGPPGASYLFYFDQPHLTMGGRLRAIEIARALVDELISGGNRAMLVSNARRSEILE
                     PLTTDRDALHARLDELAEDRDQWDLYAHNEDARVEEVVRSLND"
     gene            complement(675..1448)
                     /locus_tag="VD788_15325"
     CDS             complement(675..1448)
                     /locus_tag="VD788_15325"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV77684.1"
                     /translation="MSRLADEVVFITGASSGIGAACARAFARLGCRLLLVARRGDRLA
                     ELAGELERSGAAEVLPLVLDVRDQPRVERTIETLPDSWKRIEVLVNNAGLSRGLDALH
                     RGMLDDWEEMIDTNLKGLLYVDRAIVPLMVARKRGSVIHIGSIAGYQTYAGGNVYCAT
                     KHAVRALSDGLRLDLLGTGVRVISVDPGLVETEFSRVRFRGDEERAAAVYRDTTPLAP
                     EDVAEVVAFAATRPAHVNLAQVLVLPTDQASATHVFRRG"
     gene            complement(1519..2628)
                     /locus_tag="VD788_15330"
     CDS             complement(1519..2628)
                     /locus_tag="VD788_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77685.1"
                     /translation="MYFRCVDCGGATWVQDTPAATGSRPLTCRGCQRSYTVRPSPILQ
                     ADPRSQYETAVALAEGNGLDLPTSYSVMLGIMTLEHATAVRDQGKTAPDAAKRRTAKP
                     SSGRLVALTYDPAFEDAVRQGYMSVQQAVERGNRAEYAKRLIDRHGLTQPLAYLVADN
                     RTSLSSALQKDREEVEEILRPVKRAIWKNAVAAVLGGLAVVGVAIYGMYVWSTIEREN
                     ERVGAWAETVAEKHDRARGATPDSPAIAPGDIPARRVRVLKDDFGRVTEIEGPDPSSV
                     LLAYCSHGFGAGRFRAVELTQAVPPRPGSQLGIFEDSADPGKRFAIPIRRQLRGSRWT
                     AGDGVHPIDPIEAPELPHDARRIPVLVRDRGESDS"
     gene            complement(2724..>3176)
                     /locus_tag="VD788_15335"
     CDS             complement(2724..>3176)
                     /locus_tag="VD788_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77686.1"
                     /translation="TLEELRYNADVVCTSGACVFPDYGTGQVIFIDGDYNLGPTQSGA
                     GTLVVTGELTMQGRASWTGLILVVGEGRYLLNGAGNGEISGSLIIADIAGPDDTYNTG
                     DDCTGGTDGFDTATFDENGGGNALTQYCSTDINVANHKPYRVVEFIQR"
BASE COUNT          434 a         1139 c         1108 g          495 t
ORIGIN      
        1 gtcgttcagc gatcgaacga cttcctcgac gcgcgcgtct tcgttgtggg cgtacaggtc
       61 ccactggtcg cggtcctccg cgagctcgtc gagtctggcg tgcaacgcgt cgcggtccgt
      121 ggtcagcggc tcgaggatct cgctgcggcg ggcgttcgag acgagcatcg cgcggttgcc
      181 gcccgagatc agctcatcga ccagcgcgcg ggcgatctcg atcgcgcgca gccgtccgcc
      241 catcgtcagg tggggttggt cgaagtagaa caggtagctg gcgcccggcg ggccggccga
      301 cggcagcacc gccccggccg tgccggcggc ggtcgcggcg ccgcacagcc ggtcgagctc
      361 gaactcgtcg accctctcga gctggacgcg gatcttgaag tcgtcggccg tcagcgagcg
      421 cacgatctcg ggcggaccga cgacggtgat gtcgatctgc gccaggctgc gtccggcacg
      481 ctcctcgagc ccgatctctc gcggtcggtc gcgctccgtg cggtcggatt gagcgcgcgc
      541 cgccgtcgcg gccgacacgc acgccgccgc cagagcgatc gcgagccatc tgccggggcg
      601 gcgtcgcatc ttctccttcc cgactccgtc gaaccctcgc cgccgtccga gcgctcgcgc
      661 cgcgggcgcg gcggtcaccc gcggcggaat acgtgcgtcg ccgacgcctg gtcggtgggc
      721 agcaccagca cctgggccag gttgacgtgc gccggacgcg tcgcggcgaa cgcgaccacc
      781 tcggcgacgt cctccggcgc cagcggggtc gtgtcgcggt agacggcggc cgcgcgctct
      841 tcgtccccac ggaaccgcac gcggctgaac tcggtttcga ccagccccgg gtcgaccgag
      901 atcacgcgga cgccggtgcc gagcaggtcg agtcgcagcc cgtcgctcag cgcgcggacc
      961 gcgtgcttcg tcgcgcagta gacgttgccc ccggcgtagg tctggtaccc ggcgatcgag
     1021 ccgatgtgga tcacgctgcc ccgcttgcgg gcgaccatca gcggcacgat cgcacggtcg
     1081 acgtacagca gccccttgag gttggtgtcg atcatctcct cccagtcgtc gagcattccg
     1141 cgatggagcg cgtcgaggcc acggctgagc cccgcgttgt tcaccagcac ctcgatccgc
     1201 ttccacgagt cgggaagcgt ttcgatcgtc cgctcgacac gcggctggtc gcggacgtcg
     1261 agcaccagcg gcaggacctc ggccgctccc gaccgttcga gctcgccggc gagctctgcc
     1321 aggcgatcgc cgcgtcgcgc gacgagcaac agacggcagc ccaggcgcgc gaacgccctg
     1381 gcgcacgccg caccgatccc gctgctcgca ccggtgatga aaacgacctc gtccgcgaga
     1441 cgggacatgg cgaccctccg ccgcaccggg gaaatacgaa ggccggcacc gacaggttaa
     1501 cctaccggcg ggcgtgcttc aggagtcgga ttccccgcga tcgcggacca gcaccggaat
     1561 ccgccgtgcg tcgtgcggca gctcgggggc ttcgatcggg tcgatcgggt ggacgccgtc
     1621 gccggcggtc cagcggctgc cgcgaagctg tcggcggatc gggatcgcga accgcttgcc
     1681 gggatcggcg gaatcctcga agatgccgag ctgcgagccg ggtcgcggcg ggactgcctg
     1741 cgtcagctcg accgcgcgga agcggcccgc gccgaagccg tgcgagcagt aggcgagcag
     1801 caccgagctc gggtcgggtc cttcgatctc ggtcacccgt ccgaaatcgt ccttcagcac
     1861 gcgcacgcgc cgtgccggga tgtcgccggg cgcgatcgcc gggctgtcgg gcgtcgcccc
     1921 gcgtgcccgg tcgtgctttt cggcgaccgt ctcggcccag gcccccaccc gttcgttctc
     1981 ccgctcgatc gtcgaccaga cgtacattcc gtagatcgcg accccgacga ccgccaggcc
     2041 gcccagcacg gccgccacgg cgttcttcca gatcgctcgc ttcaccggac ggaggatctc
     2101 ctcgacctct tcacggtctt tctgcagcgc gctcgacagg ctcgtccggt tgtcggcgac
     2161 gaggtaggcg agcggctggg tcagcccgtg ccggtcgatc agacgcttgg cgtactcggc
     2221 gcgattgccg cgctcgacgg cctgctgcac cgacatgtac ccctggcgca ccgcgtcctc
     2281 gaacgcgggg tcgtaggtca gcgccacgag ccgccccgac gacggtttgg ccgtccgtcg
     2341 cttggcggca tcgggcgcgg tcttaccctg gtcgcgcacc gcggtcgcgt gctcgagagt
     2401 catgatgccg agcatcaccg aataggaggt cggcaggtcg aggccgttgc cctccgccag
     2461 ggcgaccgcg gtctcgtact gggagcgggg atcggcctgc aggatcggtg acggtcggac
     2521 cgtgtagctg cgctggcagc cgcggcaggt cagcggccgc gagcccgttg cggccggcgt
     2581 gtcctggacc cacgtcgccc cgccgcaatc cacgcaacga aagtacatcc cgctccccga
     2641 actcgcgccg gacgtgaagc gtgcggcgca ccttgccgga gcagaatcta ggtccgtcgc
     2701 gcggccgggg caaacccgcc gcgtcagcgc tggatgaact cgaccacccg gtacggcttg
     2761 tggttcgcca cgttgatgtc ggtgctgcag tactgcgtca gcgcgttacc gccgccgttc
     2821 tcgtcgaagg tcgcggtgtc gaagccgtcc gtcccgccgg tgcagtcgtc gccggtgttg
     2881 taggtgtcgt ccgggcccgc gatgtcggcg atgatcaggc ttcccgagat ctcgccgttg
     2941 ccggcgccgt tcaacaggta acgaccttcg ccgacgacca ggatcagccc ggtccacgag
     3001 gcacggccct gcatcgtgag ctcgccggtg acgaccagcg tgccggcgcc gctctgggtc
     3061 gggccgaggt tgtagtcacc gtcgatgaag atcacctgcc cggtgccgta gtcggggaag
     3121 acgcaggctc cgctggtgca gacgacgtcg gcgttgtagc gcagctcctc gagcgt
//
LOCUS       DASPGN010000527        13568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1503333, whole genome shotgun sequence.
ACCESSION   DASPGN010000527 DASPGN010000000
VERSION     DASPGN010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 13568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13568
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503333"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VD788_15340"
     CDS             <1..350
                     /locus_tag="VD788_15340"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HXV77687.1"
                     /translation="GLSPYRFVGWLMRDRLEWVPLVEYYYRTTPAALYDAGIGLVTFA
                     VWTALFGAATRCSRPVAVLAAMSLATGVEFVQTFVVGRTAGTTDILIAGLGAWAGYTI
                     RTALRLGRRPAAT"
     gene            complement(347..1558)
                     /locus_tag="VD788_15345"
     CDS             complement(347..1558)
                     /locus_tag="VD788_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77688.1"
                     /translation="MDRSRVPGSRPVVILNMHYSGLGIARDLAGTGAAVHGLSFDPSF
                     FGNYSKHCRFVVSPDTETQPEQCLDFLLDLASRCEQRPLLLVTRDHDIDFFLRFRSEL
                     DRVFVVPYPSSETLSLIMDKNRLFARARELGIRCPRECVVRSKEELVHNRERLVFPCI
                     VKPVRASQWRQRAMWRIVRRKVVKIDSYAELEAFYRTIEPHDPLVQIEEYIPGPDSDL
                     VIFGSYRNPATGVLACFTARKLIQYPSGSGTGVAVQSRRITEIEGPSKKLLDGLGYFG
                     ISEIEYKRDGRDGSYALIEMNPRHWDQHSLGAASGVNLSRALYRDLLGEPPGEAVQAD
                     REVVWLAEEGYFRSLLSNLRGGLYPWSLYAAALRGRKVFAVFDRHDLKPIVHLLRTTA
                     RQTIRQAFAKS"
     gene            1696..3123
                     /locus_tag="VD788_15350"
     CDS             1696..3123
                     /locus_tag="VD788_15350"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXV77689.1"
                     /translation="MGRGNLELWNEYGDYLIPIMPFLDSVIVRSEGSYLIDAEGNRIL
                     DLASGQFCTVLGHGHPEFTAALVDELRSNLHTGSQYVTEGVLKTAKEVADRAPGDLNK
                     VTLLSTGSEANEFALRIAKACTNRSLVAGFDRGYYGISLATRNLSRISEGKIDFTPTT
                     PGAYLIAPNCARCPIALRYPDCELSCLDLSIRLAGSSLEQCAAIIVETIVSAGGMIYP
                     TKDYLHRLRSFASEIGAMFIVDEAQTGFGRCGRWFDCENLELQPDILVFSKTSGNGYP
                     SAGVVISDEVNDRLLERGFNHLSSHQNDPLTAAAVSAVIRVIDSEGLLARSAERGEYF
                     LDRLRELESASPHLTATRGRGLMIAFELVQDKESRVPYADALVPFVLACKARGVHVTY
                     TYYEGAVRVIPPLTITQQEIDFAIDVFGEVLRDFAQGKVDKAEYQQKNHVIARVNRGR
                     GVKRLINRLWETSPEHWYRKIKQRI"
     gene            3210..3920
                     /locus_tag="VD788_15355"
     CDS             3210..3920
                     /locus_tag="VD788_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77690.1"
                     /translation="MIILTTGLSGSSPVAGLIARAGYWAGESTHKKRDYDTFENQKLI
                     ELNLKILSDVAYSGKYEMEFSRDAIDRIAESANRADLSPYADFVSLCNQHGPWIWKDP
                     RLWLTIRFWRHVLRLDDVQFIVLMRDHLQTWVSTTIRRQIHSYGYSKRYTEQVRGSIV
                     EFLDGHDQPYLELLFEDLVIHPERTLDEINGFLGTGLGMDDLKTIYTKPLYKRPRGVA
                     DFVKATMIYAKNYRSRYR"
     gene            complement(3932..7618)
                     /locus_tag="VD788_15360"
     CDS             complement(3932..7618)
                     /locus_tag="VD788_15360"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HXV77691.1"
                     /translation="MFRRVVAASVVCVVAGFWVSGAAPQENYLSFDGGDLVGIEDDAA
                     LDLAGGEFTISAWIRPTGWGGNNQGRIVDHGGGTSSLAEGWTVAVENRASSGFPQAVR
                     VQINDDSGFNGQSDSGVLSLNTWQHVAVTLAGGTLTFYVDGVERGVRTNVPTPVPSTQ
                     PIRVGNRSSDLARGFQGSIDEVRIWSRALTQQEIIDGMNVEITGGEVGLVAYYRFNSG
                     AGQNALDSGPNGLHGRLGTTILAGSTDPLWVSAAPPGNTAPSADAGPDQTVHQPATAA
                     ALDGTISDDGLPSGMLTTGWSQLSGPGTVLFGDATAVDTTATLPELGAYGLRLTVSDG
                     ELSWFDDVAVTLAPEPDLTPPLVTITEPIDQSTVSGAVTISATASDNVAVEEVEFFAD
                     GAPIATDDSEPYSVVWDSTDALTSGVHMLSAVGRDTSNNESQDSITVTVDNGLPSDLS
                     DWTVEVYSAGGSEVPDWVLEPAFPGARQELYSASCSVLLSDYELDHASAVGFSGTMGA
                     VGSDDDQFGFTFGYQERGEFYLFQWKRGDPQNPGLADIGMHIRAISTGGPDPTIEDFE
                     APGSTPTSMILEENDLRWQIGVIYDFSITFFPGGFDLEIDDPTDLNDTSWTINDVTFA
                     SGRFGFYNCSQGDAVYRLSFVDPGSCSDPDADGICGSDDNCPDDANPGQQDTDGDDVG
                     DVCDECPGFDDLIDADADGVADGCDVCPGSDDGLDADGDEVPDGCDVCAGFDDAIDSD
                     ADTVPDGCDACPGFDDSVDTDADGAPDACDVCPGSNDAIDTDADTVPDGCDVCAGFDD
                     LADADTDGVPDGCDLCPGFDDAIDTDADTVPDGCDVCAGFDDLADADTDSVPDGCDVC
                     PGFDDAIDTDVDTVPDGCDVCAGFDDLADADTDGVPDGCDLCPGFDDAIDTDADTVPD
                     GCDVCAGFDDLVDADTDGVPDGCDLCPGSNDAIDTDADTVPDGCDLCPGFDDAIDTDA
                     DTVPDGCDVCAGFDDGVDTDADTVPDGCDVCAGFNDLADADADSVPDGCDVCPGFDDA
                     IDTDADTVPDGCDLCAGFDDAIDTDADTVPDGCDTCAGFDDLADADTDGVPDGCDLCP
                     GDPTNDVDQDGVCGASDCDNDNPAVFAAPLEVTGVLVTEIDGEFRFGWAEQAGAAGPG
                     TVYDVYVGLISQVRSSGGSYQLGACEADNVTEPEFADPGLAVPPGDGLFFLIRAQNAC
                     PDGTATFGDANRDATAGTSAATCD"
     gene            complement(7704..8693)
                     /locus_tag="VD788_15365"
     CDS             complement(7704..8693)
                     /locus_tag="VD788_15365"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXV77692.1"
                     /translation="MRTLITGASGFIGSRLAYRCVELGHVVRVLTEVRNDVERQYLGE
                     LGTAGVEAVVGSVTDFDAVRRAHDGVERVFHLAAAQHEANVPDGHFWKVNVDGTRNML
                     QASVESGVSRFVHGSSIGVYGWTPNQPIRDDSALDPDNIYGVTKLAGEQAVREFAERL
                     PCVIVRISEVYGPGDRRLIKLFRGVRDGKFPIIGDGKNLHHLIYIDDLLDGLMAAAER
                     PEAVGQTFVLAGVRPVSTIEMIEAVAAELGTKPPRLRVPLAPVMLAARLAEALLRPFG
                     IQPPIHPRRMNFYRKSFAFGMTAAREAIGFAPKVDLRTGLAATKRWYVEQRIL"
     gene            complement(8713..10128)
                     /locus_tag="VD788_15370"
     CDS             complement(8713..10128)
                     /locus_tag="VD788_15370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV77693.1"
                     /translation="MNEIDLSETQERRRFDWAVIALLLLLALATRGYGLGTANLDVDE
                     YYTVYEAAERATGRTGPLLNALALASFRVFGVTEWTARLPSFVLGVITIPLFYATWRK
                     VLGRPAAVAGALIVLVSGWHLWHSQYARYYAGVFLFGSLAYYFFYRAVAKDSIRHLVA
                     GWVWLLIGTLFHPTSVLVAVAFAAYCAAVVGLPPAAGGRRPRVAKIYLWVVGVGALVA
                     LPFLWNVVSHWYLAPKQWGRSPAGLILQIVKYIQIPVAVSAAFGIVHLLRVDLRKGLF
                     FACGVVVPAGCLVLVSLGAPARPDFLFQSVPLAYAAAGYLCGVYWSTARRGLVNAALT
                     VVVLATMMPEFISHYSGRLGLHLRQVTGFLERSAQPGDRVLPFVSGIDHYSGGSLEMV
                     PRVGSPFTQRADWSEVMDAAAGLPGRLWIVIPGARDPLAPGLETWLRDHAWLKWRRFE
                     KRFDYTHHGYEVYLVVDESNE"
     gene            complement(10125..11288)
                     /locus_tag="VD788_15375"
     CDS             complement(10125..11288)
                     /locus_tag="VD788_15375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV77694.1"
                     /translation="MRILFCNFEYPPLGGGGGVINALLAEELATRHDVTLLTSGTGDL
                     PLDSVERGVRVLRVPVLFRRRQATASMISMLSYVLRAPGVGRRQMHERGGFDVVNTHF
                     VLPSGPVGDRLARGAGIPNVLSVHGGDLYDPSKITSPHRHPPLRAWIRRLSRRADAVV
                     GQSTNTLENLKRFYAPEVQGIRIPLGIRRPPGGTGSRGAYQFGDDDVLLVTVGRIVAR
                     KAINQLVSMMARLDSPRARLVVIGDGPVLPALRKQAAEAKLGDRVRFLGRLDEAEKFR
                     VLRFCDLFVSTSQHEGFGLVFLEGMACGLPVVCYDHGGQTDFLADGRTGHLVPLNDIA
                     AFTERCRALIDDHERRRVMGSVNATEVAEYFIENCAARYEELFERVIRGAVRR"
     gene            11359..12096
                     /locus_tag="VD788_15380"
     CDS             11359..12096
                     /locus_tag="VD788_15380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV77695.1"
                     /translation="MPVVRELSARTEPFDSYWQAPKDIERGYTSFRQYYRANFLPHLP
                     PDRAAKILVVSCGPGYLVQLLKDEGYTAVVGIDSDPDKIGYAERRKLPCRVAEAFPFL
                     ESVADAYDLILCEQELNHLTKDETIEFLRLCHRALRPGGGLLVYGLNGANPITGAEAL
                     SQNFDHFYTFTEYSLLQILELCGFEQCRALPLKLYVFYRNPLNYVGWGATILLETIFR
                     VLFRLYGKSAKIFTKKIAAVCRKPRQS"
     gene            complement(12050..>13568)
                     /locus_tag="VD788_15385"
     CDS             complement(12050..>13568)
                     /locus_tag="VD788_15385"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV77696.1"
                     /translation="DLHRIRESCQERSSLLRIAPHLVRPLPILVPTYRGLMKGRMAMR
                     TALAIYDAITWDRNRGVPSWNRIPRGRILSRRECLELYPALDRPELTGGALFHDGQMY
                     NQTRLGLAFVRSAAERGAAVANYAEVAAFLREGDRIVGVRVTDRIGGGTFDVRGRVVI
                     NAAGPWAEPLLRRSGLSLERTPTFSRDACFVVPRRLTPDTALAIQGATNDPDALLSRG
                     ERHLFLVPWRHCTLVGVWHVVHRGAPDDFTVTRADLDGFIAEINGALPGLDLTHDDVA
                     LWNAGLVLFGENDPRARNLSYGKRSWIVDHERIDGIADLLTVIGVRYTTARGVARQTV
                     DRAMRKMGQAPNRSPTAHAPIWGGDVDDLSQVVREAAGAGLEAPVADALAHNYGSRFK
                     DVLAEAAGRPELLRPIGGTTTLRAEVLHAVRREMAQCLEDVVLRRTDLGTADDPGDAA
                     LHQVADIVAAELGWDAARSRSEVERLRSHYLGRRGTDRSPVTIDAACDRPPRSSS"
BASE COUNT         2305 a         4566 c         4522 g         2175 t
ORIGIN      
        1 cgggcttgag tccgtaccgg ttcgtcgggt ggttgatgcg tgaccgactc gaatgggtcc
       61 cgctcgtcga gtactactac cgcacgaccc cggcggcgct gtacgacgcc ggtatcgggc
      121 tcgtcacctt cgccgtctgg accgcgctgt tcggcgcggc gacgcggtgc tcccggccgg
      181 tcgccgtgct cgccgccatg tcgctcgcca ccggcgtcga gttcgtccag acgttcgtcg
      241 tcgggcgcac ggcgggaacg accgacatcc tgatcgccgg tctcggggcg tgggccggct
      301 acacgatccg gacggcgctc cgtctcggcc gtcgcccggc cgccacctag ctcttggcga
      361 aggcctgccg gatcgtctgg cgggccgtcg tgcgcagcaa gtgaacgatc ggcttcaggt
      421 cgtggcggtc gaagaccgcg aacaccttcc tcccgcgcag ggcggccgcg tacaacgacc
      481 acggatagag gcctccccgc aggttgctca gcaacgagcg aaagtagcct tcctcggcga
      541 gccagaccac ctcgcggtcg gcttggacgg cctcgccggg cggctcgccg agcagatcgc
      601 ggtagagcgc ccgcgacagg ttgacgccgc tcgcggcgcc cagcgagtgc tggtcccagt
      661 ggcggggatt catctcgatc agcgcgtaac tgccgtcgcg accgtcgcgc ttgtactcga
      721 tctcggagat gccgaagtag cccaatccgt cgagcagctt cttgctcggc ccctcgatct
      781 ccgtgatgcg ccgactctgg acggcaactc cggtgccgga gcccgaaggg tattggatca
      841 gcttccgcgc ggtgaaacag gcgagcacgc cggttgccgg gttgcgatac gagccgaata
      901 tcaccaggtc cgagtcgggg ccggggatgt actcctcgat ctgcaccagc gggtcgtggg
      961 gctcgatcgt tcggtagaac gcctccagct cggcgtacga gtcgatcttg accaccttgc
     1021 gccggacgat tcgccacatt gctcgttgcc gccactgcga cgcgcggacg ggcttgacga
     1081 tgcaggggaa gaccagacgc tcgcggttgt ggaccagttc ctccttcgat cgcacgacgc
     1141 actcgcgcgg gcaacggatg ccgagctcgc gggcccgagc gaacagacgg ttcttgtcca
     1201 tgatcagcga cagcgtctcg gaagacggat acggtacgac gaacacccgg tcgagttcgg
     1261 aacggaaccg caggaagaaa tcgatgtcgt ggtcgcgcgt cacgagcagg agcggtcgct
     1321 gctcgcagcg ggacgccagg tcgagcagga agtccaggca ttgttcgggc tgcgtctcgg
     1381 tatcgggcga gacgacgaag cggcagtgtt tggagtagtt accgaagaac gacggatcga
     1441 agctcaggcc gtggaccgcc gctcccgtcc cggcgaggtc gcgcgcgatg ccgaggcccg
     1501 agtagtgcat gttgaggatc accaccggac gggacccggg cactcgcgat cgatccatcg
     1561 gccaaatcta ctaagaagcc attccgattg ccagaccgcc cactcgctcg cgctcggctc
     1621 ggccgacatc gtctatactc cgtcccgccg gcgcgcgacg agaacgatgg actgcacggt
     1681 cgacggagat gttgaatggg ccgcggaaac ctggagctgt ggaacgagta cggtgactac
     1741 ctgataccga tcatgccctt cctcgacagc gtgatcgtgc gcagcgaggg ctcgtacctg
     1801 atcgacgccg agggcaacag gatcctcgat ctcgcctccg gccagttctg taccgtgctc
     1861 ggacacggtc atccggagtt caccgcggcg ctcgtcgacg aattgcgcag caaccttcac
     1921 accggatccc agtacgtgac ggaaggcgtg ctgaagacgg ccaaggaggt cgcggaccgc
     1981 gctcccggcg acctgaacaa ggtgaccctg ctatcgacgg gatcggaggc caacgagttc
     2041 gcgttgcgca tcgcgaaggc gtgcacgaac cgaagcctgg tcgccggctt cgaccgtggc
     2101 tattacggca tctcgctggc gacgcgcaat ctcagccgga tctccgaagg caagatcgac
     2161 ttcacaccga cgacccccgg tgcgtacttg atcgccccga actgtgccag atgtccgatc
     2221 gccctgcgct atcccgactg cgagttgagc tgcctcgatc tgtcgattcg tctggctggt
     2281 tcgtcgctcg agcagtgcgc ggcgatcatc gtcgagacga tcgtatcggc cgggggaatg
     2341 atctacccga cgaaggatta cctgcaccga ctgcgctcgt tcgcgagcga gatcggcgcg
     2401 atgttcatcg tcgacgaagc acagaccggg ttcgggcgct gcggccggtg gttcgactgc
     2461 gagaacctcg agctgcagcc ggacattctc gtcttttcga agacctccgg aaatgggtat
     2521 ccgtcggcgg gggtcgtcat ctcggatgag gtcaacgacc ggctcctcga gcgcggattc
     2581 aaccacctgt cgagccatca gaacgacccg ctgacggcgg cggcggtgtc ggcggtgatc
     2641 cgggtcatcg actcggaggg tctgctggcg agatccgccg agcggggcga gtacttcctc
     2701 gaccgcctgc gcgagctcga gagcgcttcc ccgcatctga cggcgacgcg cgggcgcggg
     2761 ctgatgatcg cgttcgagct ggtgcaggac aaggagagcc gcgtcccgta cgccgacgcg
     2821 ttggttccgt tcgtgctcgc gtgcaaggcg cgaggggtcc acgtgaccta cacgtattac
     2881 gagggcgcgg tacgggtcat cccacccctg accatcacgc agcaggagat cgatttcgcg
     2941 atcgacgtgt tcggggaggt tctccgcgac ttcgcgcagg ggaaggtcga caaggcggag
     3001 tatcaacaga agaaccacgt gatcgccagg gtcaaccgcg gccgcggcgt gaagcgcctg
     3061 atcaatcgac tgtgggagac cagtcccgag cactggtacc gcaagatcaa gcagcgtatc
     3121 tgagacattt cgccgggatt cagcgatcta caccggcgag agggacggct ccagtgaacg
     3181 acgcctcgcg aaccccaccg aagaagaacg tgatcatctt gacgaccggg ctgtccggta
     3241 gctcgccggt tgccgggctg atcgcccgcg cgggctactg ggcgggggag tcgacccaca
     3301 agaagcgcga ctacgacacg tttgagaacc agaagctgat cgagctcaac ctgaagatct
     3361 tgtcggacgt ggcctattcg ggcaagtacg agatggagtt ctcgcgcgac gcgatcgacc
     3421 gaatcgcgga gtcggcgaat cgggccgatc tctcgcccta cgccgacttc gtcagtctgt
     3481 gcaatcaaca cggtccgtgg atctggaagg atccgcggtt gtggttgacg atccggttct
     3541 ggcgtcacgt gctccggctc gacgacgtcc agttcatcgt gctgatgcga gatcacctgc
     3601 agacgtgggt gtcgacgacg atccggcggc agattcactc gtacggctat tccaagcggt
     3661 ataccgagca ggtgcgcggc tcgatcgtcg agttcctcga cggtcacgat cagccgtatc
     3721 tggagctgtt gttcgaggac ctggtgatcc acccggagag aacactcgac gagatcaacg
     3781 gctttctcgg gaccgggctg ggcatggacg atctgaagac gatctacacc aagccgctgt
     3841 acaagcgacc ccgtggcgtc gccgacttcg tcaaggccac gatgatctac gcgaagaact
     3901 accggtcgcg gtaccgctaa cggccgggcc ctcagtcgca ggtggccgcg ctcgtgccgg
     3961 cggtcgcgtc tcgattggcg tcgccgaagg tcgccgttcc gtccgggcac gcgttctgcg
     4021 cgcggatcag gaagaacagg ccgtcacccg gcgggaccgc gaggcccgga tcggcgaact
     4081 ccggctccgt gacgttgtcg gcctcgcacg ctccgagctg gtacgaacca cccgacgagc
     4141 ggacctgcga gatcagaccg acgtagacgt cgtagacggt accgggtccg gccgcgcccg
     4201 cctgctcggc ccagccgaat cggaattcgc cgtcgatctc ggtcacgagc accccggtga
     4261 cttcgagcgg tgccgcgaac acggccgggt tgtcgttatc gcagtccgaa gcaccgcaga
     4321 ccccgtcttg gtcgacgtcg ttcgtcgggt cgccggggca gagatcgcag ccgtcgggca
     4381 cgccgtcggt gtccgcatcg gccaggtcgt cgaacccggc acaagtgtca cagccgtcgg
     4441 gcacggtgtc ggcatcggtg tcgatcgcgt cgtcgaatcc ggcgcagagg tcacacccgt
     4501 ctggcacggt atcggcatcg gtgtcgatcg catcgtcgaa gcccgggcaa acgtcgcagc
     4561 catcgggcac gctgtcggcg tcggcgtcag cgaggtcgtt gaacccggcg caaacgtcac
     4621 agccatcggg cacggtatcc gcatcggtat ccacgccgtc gtcgaacccg gcgcagacgt
     4681 cacagccgtc gggcacggtg tcggcatcgg tgtcgatcgc gtcgtcgaat ccggggcaga
     4741 ggtcacaccc gtctggcacg gtgtcggcat cggtgtcgat cgcgtcgttc gagccggggc
     4801 agaggtcgca gccgtccggc acgccgtcgg tgtcggcatc gacgaggtcg tcgaacccgg
     4861 cgcaaacgtc gcagccatcc gggacggtat cggcatcggt gtcgatcgca tcgtcgaatc
     4921 cggggcagag gtcgcagccg tccggcacgc cgtcggtgtc ggcatcggcg aggtcgtcga
     4981 acccggcgca aacgtcgcag ccatccggga cggtgtcgac gtcggtgtcg attgcgtcgt
     5041 cgaagcccgg gcaaacgtcg cagccatcgg gcacgctgtc ggtgtcggcg tcggccaggt
     5101 cgtcgaaccc ggcgcacacg tcgcagccgt cgggtacggt gtcggcatcg gtgtcgatcg
     5161 cgtcgtcgaa tccggggcag aggtcacaac cgtccggcac gccgtcggtg tcggcgtcgg
     5221 ccaggtcgtc gaacccggcg cagacgtcgc agccgtcggg gacggtatcg gcgtcggtgt
     5281 cgatcgcgtc gttcgagccg ggacagacgt cgcacgcgtc cggcgcccca tcggcgtcgg
     5341 tatcgaccga gtcgtcgaat cccgggcacg catcgcagcc gtcgggcacc gtgtccgcat
     5401 cgctgtcgat cgcgtcgtcg aaaccggcgc agacgtcaca accgtcgggg acttcgtcgc
     5461 cgtcggcatc caggccgtcg tcggagccgg ggcagacgtc gcagccgtcc gccacgccgt
     5521 ccgcgtcggc gtcgatcagg tcgtcgaacc ccgggcactc gtcgcagacg tctcccacgt
     5581 cgtcgccgtc ggtatcctgc tgaccgggat tggcgtcgtc ggggcagttg tcgtccgagc
     5641 cgcagatgcc gtcggcgtcg gggtcgctgc acgatccggg atcgacgaac gacaggcggt
     5701 agaccgcgtc cccttgggag cagttgtaga agccgaaccg gccgctcgcg aaggtgacgt
     5761 cgttgatcgt ccaggacgtg tcgttcaggt cggtcgggtc gtcgatctcg aggtcgaagc
     5821 cgccgggaaa gaacgtgatc gagaagtcat agatgactcc gatctgccag cgaaggtcgt
     5881 tctcttccag gatcatcgac gtgggcgtgg accccggtgc ttcgaagtcc tcgatcgtcg
     5941 gatccgggcc gccggtcgag atcgcccgga tgtgcatccc gatgtcggca agaccggggt
     6001 tctgcgggtc gccccgcttc cactggaaca gatagaactc gccgcgctcc tggtagccga
     6061 aggtgaatcc gaattggtcg tcgtcactcc cgacggcgcc catcgtcccg ctgaacccga
     6121 cggcgctcgc atgatcgagc tcgtagtcgc tgagcaggac cgagcacgac gcggagtaca
     6181 gctcctgccg agcgcccgga aacgccggct cgagcaccca atccggtacc tcggatccac
     6241 cggccgagta gacctcgacg gtccagtcgg aaaggtcgct cggcaggccg ttgtcgacgg
     6301 tcacggtgat cgagtcctgc gactcgttgt tcgacgtatc gcgaccgacg gccgacagca
     6361 tgtgcacacc ggacgtcagt gcgtcggtgc tgtcccagac gacgctgtac ggctcgctgt
     6421 cgtcggtcgc gatcggcgcg ccgtccgcga agaattccac ctcctcgacg gcgacgttgt
     6481 cgctcgcggt cgccgagatc gtcaccgccc cgctgaccgt gctctgatcg atcggttcgg
     6541 tgatcgtcac gagtgggggg gtgaggtccg gttccggcgc cagcgtgacc gccacgtcgt
     6601 cgaaccacga cagctcgccg tcgctgacgg tcaaccgtag gccgtaggct cccagctcgg
     6661 gtagcgtggc cgtcgtgtcg accgccgtag cgtcgccgaa cagcacggtc cccgggccgc
     6721 tcaactggga ccagccggtg gtgagcatgc ccgacggcag gccgtcgtcg ctgatcgtcc
     6781 cgtccagggc ggcggcggtc gccggctgat gcacggtctg atccggaccg gcgtcggccg
     6841 acggcgccgt gttgcccggc ggcgccgcac tgacccagag cggatcggtc gagccggcga
     6901 ggatcgtcgt accgaggcgg ccgtgcagcc cgttgggccc gctgtcgagc gcgttctgcc
     6961 cggcgcccga attgaagcgg tagtaagcga cgagcccgac ctcgcccccc gtgatctcga
     7021 cgttcatccc gtcgatgatt tcctgttggg tcagtgcgcg gctccagatc cgcacctcgt
     7081 cgatcgagcc ttggaagccc cgtgcgagat cggacgaccg attcccgacg cggatcggct
     7141 gggtcgaggg aaccggcgtg gggacgttgg tccgcacgcc gcgctcgacg ccgtcgacgt
     7201 agaacgtcaa cgtcccgccg gcgagcgtga ccgccacgtg ctgccacgtg ttgaggctca
     7261 gcacgccgga atcggactga ccgttgaagc cggagtcgtc gttgatctgg acccgaaccg
     7321 cctgcgggaa gcccgacgat gctcggttct cgacggcgac ggtccagccc tcggccaagc
     7381 tcgacgtgcc gccaccgtgg tcgacgatgc ggccttggtt gttgccgccc cagccggtcg
     7441 ggcgaatcca cgcgctgatc gtgaactctc cgccggcgag gtcgagcgcc gcgtcgtctt
     7501 cgatcccgac gaggtcgccg ccgtcgaagc tcagatagtt ctcttggggg gccgcacccg
     7561 agacccagaa tcccgcaaca acgcagacaa cggacgcggc tacgactcgc cggaacacgc
     7621 cccggtcccc ctgtatccca actgaccggc ttcagtctac gtccgaactc gcgccgccgc
     7681 tgcccgcgat ggccgcgggt tgttcagagg atgcgctgct cgacgtacca gcgcttggtc
     7741 gcggcgagcc ccgtgcggag atcgaccttg ggtgcgaagc cgatcgcctc gcgcgccgcg
     7801 gtcatcccga aggcgaagct cttgcggtag aagttcatcc gccgaggatg gatcggcggc
     7861 tggatgccga acgggcgcag caacgcctcc gccagccggg cggcgagcat caccggcgcg
     7921 agcgggaccc gcaggcgggg gggcttagtg cccaactcgg cggccaccgc ctcgatcatc
     7981 tcgatcgtcg agaccggacg caccccggcg agaacgaacg tctggccgac cgcctccggt
     8041 cgttcggccg ccgccatcag gccgtcgagc aggtcgtcga tgtagatcag gtggtgcagg
     8101 ttcttcccgt ccccgatgat cggaaacttg ccgtcacgca cgccccggaa cagcttgatc
     8161 agtcttcgat caccgggtcc gtagacctcg gaaatgcgga cgatgacgca aggcagccgt
     8221 tccgcgaatt cgcgcaccgc ctgctcgccg gccagcttcg tgaccccgta gatgttgtcg
     8281 gggtcgagcg ccgaatcgtc gcggatcggc tgattcgggg tccaaccgta aaccccgatc
     8341 gagctgccgt gaacgaaccg gctcacgccg gattcgacgc tcgcctgcag catgttgcgg
     8401 gttccgtcga cgttgacctt ccagaaatga ccgtcgggga cgttcgcctc gtgctgcgcc
     8461 gccgccaggt ggaatacccg ctcgaccccg tcgtgggccc gccggaccgc gtcgaaatcg
     8521 gtgaccgacc cgacgaccgc ttcgacgccg gcggttccca gctcacccaa gtactgccgc
     8581 tcgacgtcgt tccggacctc ggtcagcact cgcacgacgt ggcccagctc cacgcagcga
     8641 tacgccaagc gcgagccgat gaagccactc gctccggtaa tcaacgtcct catcgaccta
     8701 cacctcgccc gatcactcgt tcgactcgtc gacgacgagg tagacctcgt agccgtggtg
     8761 cgtgtagtcg aaccgcttct cgaagcgacg ccatttcaac cacgcgtgat ccctgagcca
     8821 cgtctcgagc cccggagcca gcggatcccg cgctcccggt atcacgatcc aaagccgacc
     8881 cggcagcccg gccgccgcgt ccatcacttc gctccagtcc gcgcgctgcg tgaacgggct
     8941 gccgacccgc ggcaccatct cgagcgagcc gccggagtag tggtcgatgc ccgagacgaa
     9001 gggaagtact cgatcgccgg gctgcgcgga tcgctcgagg aagcctgtga cctgtcgcag
     9061 atggagaccc agacgcccgc tgtagtgcga gatgaactcg ggcatcatcg tcgccagcac
     9121 gacgacggtc agcgccgcgt tgaccagccc gcgccgtgcc gtgctccagt acaccccgca
     9181 caggtagccc gcggccgcgt aggccagcgg caccgactgg aacaggaagt cgggtcgcgc
     9241 aggcgcgccg agcgacacca gcaccaaaca accggcgggc acgacgaccc cgcaggcgaa
     9301 gaacagaccc ttgcgcaaat cgacccgcag caggtggacg atgccgaacg ccgcgctgac
     9361 cgcgaccggg atctggatgt acttgacgat ctgcaggatc agcccggccg gagatcgccc
     9421 ccattgcttc ggcgcgaggt accagtggct tacgacgttc cacaggaacg gaagagcgac
     9481 gagcgcgccg acgccgacga cccacaggta gatcttggcg acgcgcggtc tgcggccccc
     9541 ggcggccgga ggaagcccca cgaccgcggc gcagtaggcg gcgaaggcaa ccgcgacgag
     9601 caccgacgtc ggatggaaca acgtgccgat caggagccag acccagccgg cgacgaggtg
     9661 acggatgctg tccttggcca ccgcccggta gaagaagtag taggcgagcg agccgaacaa
     9721 gaagacgccc gcgtagtacc gtgcgtactg cgaatgccaa aggtgccagc cgctgaccag
     9781 cacgatcaac gcgccggcga ccgccgccgg gcgtcccagg accttgcgcc acgtcgcgta
     9841 gaacagcgga atggtgatca cgccgagaac gaacgacggc agtctggccg tccactcggt
     9901 gacaccgaag acgcggaagc tggcgagcgc cagcgcgttc agcaacggac cggtgcgacc
     9961 ggtcgctcgc tcggcggcct cgtagacggt gtagtactcg tcgacgtcga ggttggccgt
    10021 gcccaggccg tagccccggg tcgcgagcgc cagcagcaac aacagcgcga tcaccgccca
    10081 gtcgaaccga cggcgctcct gcgtttcgga aaggtcgatc tcgttcatcg gcggacggct
    10141 cctcggatca cccgttcgaa cagctcttcg tagcgtgcgg cgcagttctc gatgaaatac
    10201 tcggccactt cggtcgcgtt gacgcttccc atcactcggc gccgctcatg atcgtcgatg
    10261 agcgcccggc agcgctcggt gaacgccgcg atgtcgttga gcgggacgag atgtccggtc
    10321 cggccgtcgg cgaggaaatc ggtctggccg ccgtggtcgt aacagaccac cggcaggccg
    10381 caggccatcc cctcgagaaa gaccaggccg aagccctcgt gctggctggt cgatacgaac
    10441 agatcgcaaa aacgcagcac gcggaacttc tccgcttcgt ccaggcggcc gagaaagcgg
    10501 acacggtcgc cgagcttcgc ctcggcggct tgcttgcgca gcgcgggaag caccggcccg
    10561 tcaccgatca ccaccagcct ggcgcgcggc gagtccaagc gagccatcat cgacaccagc
    10621 tggttgatcg ccttcctcgc gacgattcga ccgacggtca cgagcagcac gtcgtcgtcg
    10681 ccgaactggt acgcgcccct cgagccggtg ccgcccgggg gccggcggat gcccaacgga
    10741 atgcggatgc cttggacctc cggagcgtag aaccgtttga ggttctcgag cgtgttggtc
    10801 gactgcccga cgacagcgtc cgcgcggcgg ctcaaccggc gaatccacgc gcgcagcggc
    10861 ggatgacgat ggggcgaggt gatcttgctc ggatcgtaca ggtccccgcc gtggaccgac
    10921 agcacgttgg ggatacctgc gccgcgggcc aggcgatcgc cgaccgggcc cgacggcagg
    10981 acgaagtggg tgttgaccac gtcgaagccg ccgcgttcgt gcatctgccg tcgtccgacc
    11041 cccggggcac gcagcacgta cgacagcatc gagatcatcg aagcggtcgc ctgccggcgg
    11101 cggaacagga ccggaacccg cagcacgcgc actcctcgtt cgaccgagtc cagcggcaag
    11161 tccccggtgc ccgaggtcaa gagcgtcacg tcgtggcggg tcgccagctc ttcggcgagc
    11221 agcgcgttga tcacccctcc gccgcctccc agcggagggt attcgaagtt gcagaagaga
    11281 atgcgcatgc caacccccgc gcggcattat gatgctcgtc gcggcgtacc ccgcccgccg
    11341 agctgaggag catataccat gcccgtcgtc agagaactgt ccgcgcggac cgagccgttc
    11401 gattcctatt ggcaagcccc gaaggacatc gagcgaggct acacctcctt tcgccagtac
    11461 taccgcgcga acttccttcc gcacctgccg ccggatcgtg ccgccaagat cctcgtcgtc
    11521 agctgcgggc cgggctacct ggttcagcta ctcaaggacg agggctatac cgcggtcgtc
    11581 ggcatcgact ccgatcccga caagatcggt tacgccgagc ggcgcaagct gccttgtcgg
    11641 gtggccgagg ccttcccgtt cctcgagtcg gtcgccgacg cgtacgacct gatcctgtgc
    11701 gagcaggagc tgaatcacct gaccaaggac gagacgatcg agttcttgcg gttgtgtcac
    11761 cgggcgcttc gcccgggcgg agggctgctg gtctatggcc tcaacggtgc gaatccgatc
    11821 accggcgccg aagcgctgtc gcagaacttc gaccatttct acaccttcac cgagtacagc
    11881 ctgctgcaga ttctcgagtt gtgcggattc gagcaatgcc gggcactgcc gctcaagctc
    11941 tacgtcttct atcgcaaccc gctgaactac gtcggctggg gcgcgacgat cttgctcgag
    12001 acgatcttcc gcgtcttgtt tcgcctctat ggcaagtcgg cgaagatctt cacgaagaag
    12061 atcgcggcgg tctgtcgcaa gccgcgtcaa tcgtgacggg cgatcgatcg gtgccgcgcc
    12121 gtccgaggta gtgggaccgc aggcgctcca cctcggagcg cgagcgcgcc gcatcccagc
    12181 ccagctccgc tgcgacgatg tcggcgactt ggtggagcgc cgcgtcgccc gggtcgtccg
    12241 cggtccccag atccgttcga cggagcacga cgtcttccaa gcactgcgcc atctcgcgcc
    12301 ggaccgcgtg cagcacttcc gcgcggaggg tcgtcgtccc accgatcggt cgcaacagct
    12361 ccggccggcc cgccgcctcg gccagcacgt ccttgaaccg agagccgtag ttgtgcgcca
    12421 gcgcgtcggc gaccggggcc tcgaggccgg cgcccgccgc ctcccgtacg acctgcgaca
    12481 gatcgtcgac gtcgccgccc cagatcggcg cgtgcgccgt cggcgagcgg ttcggcgcct
    12541 gacccatctt gcgcatcgct cgatcgacgg tctgccgcgc cacccccctg gcggtcgtgt
    12601 accgcacgcc gatcacggtc aacaggtcgg cgatgccgtc gatccgctcg tgatcgacga
    12661 tccacgagcg cttgccgtaa ctcaggtttc gcgcgcgcgg gtcgttttcg ccgaacagga
    12721 cgaggccggc gttccacagc gcgacgtcgt cgtgggtcag gtccaaaccg gggagcgcgc
    12781 cgttgatctc ggcgatgaac ccgtcgaggt cggctcgggt caccgtgaaa tcgtccggtg
    12841 cgccgcgatg gacgacgtgc cagactccga ccagcgtgca gtgtcgccag ggaacgagaa
    12901 agagatgacg ctcgccgcgg ctcagcagcg cgtcggggtc gttcgtcgcg ccctgaatcg
    12961 cgagcgccgt gtccggcgtc aggcgccgcg gcacgacgaa gcaagcgtcc ctggaaaagg
    13021 tcggcgtgcg ctcgagtgac aggcccgagc gtcgcaagag cggttcggcc cacgggcccg
    13081 cggcgttgat cacgactcgg ccgcgaacgt cgaaggtgcc gccgccgatc cgatcggtga
    13141 ccctgacccc gacgatgcga tcgccttccc gcaggaaggc ggcgacctcg gcgtagttgg
    13201 ccaccgcggc accgcgctcc gcggccgagc gaacgaaggc gagtccgagg cgggtctggt
    13261 tgtacatctg accgtcgtga aacagcgccc cgccggtcag ctccggccga tcgagcgcgg
    13321 ggtacagctc gaggcattcg cgccgggaga ggatccgtcc gcgggggatg cgattccacg
    13381 acggaacgcc gcgattgcgg tcccaggtga tcgcgtcgta gatcgccaac gcggtccgca
    13441 tcgccatgcg acccttcatc agaccgcggt aggtcggcac caggatcggc agcggacgga
    13501 cgagatgcgg ggcgatgcgc aacagcgagc tgcgctcctg gcaggactcg cggatccgat
    13561 gaagatcg
//
LOCUS       DASPGN010000528         3695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_334290, whole genome shotgun sequence.
ACCESSION   DASPGN010000528 DASPGN010000000
VERSION     DASPGN010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334290"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VD788_15390"
     CDS             complement(<1..583)
                     /locus_tag="VD788_15390"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXV77697.1"
                     /translation="MIRSRPLPAVAVPPVTRDPEVVAAYLDDASGAPRGHACGLVRVE
                     SEGEAAALLAATRAQATPILCQAARTSLTGGAIPYGELVVSVERMDSIGPVEATGDSA
                     TVRVEPGVRLATLGSELARRGWYYPPTPTYRQAMIGGSVATNAGGAATFKYGVTRQWV
                     RALRVLLWNGELIEVERGQLQAARGGRFTVVSAD"
     gene            complement(609..947)
                     /locus_tag="VD788_15395"
     CDS             complement(609..947)
                     /locus_tag="VD788_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77698.1"
                     /translation="MLEMSVVALVVLVGAFLCLVLLPLFLIAAAVKLLVVLILLPFRL
                     IAGLAAVLAAVLATLGKALFLLLAVALGPLVLIALLLAVPLLPILAAIACVVVIARLG
                     RPRATPAGAS"
     gene            1066..2007
                     /locus_tag="VD788_15400"
     CDS             1066..2007
                     /locus_tag="VD788_15400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXV77699.1"
                     /translation="MPTQKRHTHPHPGAADPAGARRLAWVLALTVAYALAEVLGGWLA
                     NSLALLADAGHMVTDVMALALALVAARWSRRPPDASRTYGYRRVEILAALFNGVGLIL
                     VALAIVYEAWQRLDESPQIDVSLMAAIAAGGLVVNLVAAGILHGGHARLNVRAAYLHV
                     VGDLLGSIGALIAAGLIWRFDWIWADAVASAVICAIIVFSAVRLVLESVNVLLEGAPS
                     GIRTEEVARCLLETPGVCDVHDLHLWSLGAGSPLLTAHLVMDHSEPADRVLRAASDAL
                     LTRFGVTHSTLQIEPPDYNIVQQLTPEVAPSGSTADD"
     gene            complement(2009..3523)
                     /locus_tag="VD788_15405"
     CDS             complement(2009..3523)
                     /locus_tag="VD788_15405"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXV77700.1"
                     /translation="MSDDVLDALGIEPVNAGACTGEWFDTRGSELISYNPANEEPIAT
                     VRQASAADYERVAEAAQRAFLGWRMTPAPRRGEIVRQLGEELRIHKEPLGRLVSIEMG
                     KILTEGLGEVQEMIDVCDFAVGLSRQLYGLTMHSERSRHRMYEQWHPLGPVGIVTAFN
                     FPVAVWSWNAAIAAVCGDTMIWKPSSQTPLTAVAVQNICNRVMQRNGLSGVFNLIVGR
                     GADVGERMLADVRLPLISATGSCRMGRRINEVVGQRLGRALLELGGNNAVVVMDDADQ
                     ELALRAVLFGAVGTAGQRCTTTRRLILQQGIAADFTAKLVRAYASVPIGDPLDKTTLM
                     GPLVNEAAVRDMEQALAKVAEQGGKVICGGKTIDRPGHFVQPTIVGISPDADIVKHET
                     FAPILYVHEFDDLEQAIRIHNDVPQGLSSSIFTTSVIAAETFVSHAGSDCGIANVNVG
                     TSGAEIGGAFGGEKETGGGRESGSDSWKWYMRRQTNTINWGSEMPLAQGVTFEL"
BASE COUNT          578 a         1325 c         1245 g          547 t
ORIGIN      
        1 cgtcggccga gacgacggtg aaacgtccgc cgcgggcagc ctgcagctga ccccgctcga
       61 cctcgatcag ttcgccgttc cacagcagca cgcgcagcgc tcggacccac tgcctcgtca
      121 cgccgtactt gaacgtggcg gcaccaccgg cgttcgtcgc cacgctgcca ccgatcatcg
      181 cctgccggta ggtcggcgtc ggcgggtagt accagccacg tcgggccagc tcactcccga
      241 gcgtcgcgag tcgcacaccc ggctcgacgc gaaccgtcgc cgagtcgccg gtcgcctcga
      301 cgggaccgat cgaatccatc cgctcgacgg aaacgaccaa ctcgccgtac gggatcgccc
      361 caccggtgag cgacgttcgc gccgcctggc acaggatcgg cgtcgcctgc gcccgcgtcg
      421 cggcgagcag cgccgccgcc tcgccctcgg actcgacgcg aaccagaccg caggcgtgcc
      481 cgcgcggtgc cccggacgcg tcgtcgaggt aggcggcgac gacttccggg tcgcgcgtga
      541 cgggaggcac ggcgacggcc ggcaatggcc gcgagcggat catcggcaac cctgcccgcg
      601 gcgggtgctc agctcgcgcc ggcgggcgtg gcccgcggcc gtcccaggcg cgcgatcacg
      661 acgacgcagg cgatcgccgc caggatcggc agcagcggaa cggcgagcag cagcgcgatc
      721 agcacgagcg ggcccagcgc gacggccagc aacaggaaca gcgccttgcc cagggtggcg
      781 agcacggcgg ccaggaccgc ggccagaccg gcgatcagcc ggaacggcag caggatcagc
      841 acgaccagca gcttgacggc cgccgcgatc agaaacagcg gcagcagcac cagacagagg
      901 aacgcaccga cgagaacgac cagggcgacg acggacatct cgagcatctg ccgcactccc
      961 gcggggcggg ccggccggcc ccgtcaccgg tgagtacgga cgaccggccg cgaccgttcc
     1021 gaaaaaaaga ccggttggtt taacctgatc accccggagg gaatcgtgcc aacccaaaag
     1081 cgccacaccc acccgcatcc cggcgcggcc gatccggccg gcgcccgccg gctggcctgg
     1141 gtgttggcgc tgacggtggc ctacgcgctg gccgaggtgc tcggcggctg gttggccaac
     1201 agcctggcgc tgctggccga tgccggccac atggtcaccg acgtgatggc gctggcactg
     1261 gccttggtcg ccgcccgctg gtcgcggcgc ccgccggatg cgagccgcac ctacggctac
     1321 cgccgggtcg agatcctggc cgcactgttc aacggcgtcg ggctgatctt ggtcgcgctg
     1381 gcgatcgtct acgaagcctg gcagcgtctc gacgaatcgc cccagatcga cgtctctctg
     1441 atggccgcga tcgcggcggg cgggctcgtc gtcaacctcg tggcagccgg catcctccac
     1501 ggaggtcacg cccggctcaa cgtccgcgcg gcctatcttc acgtggtcgg cgatctgctc
     1561 ggatcgatcg gcgcgttgat cgcggccggt ctgatctggc gtttcgactg gatctgggcc
     1621 gacgccgtcg ccagcgccgt catctgcgcc atcatcgttt tcagcgcggt gcgcttggtg
     1681 ctcgagtcgg tcaacgtgct gctcgagggc gcgccgtccg ggattcgcac cgaggaggtc
     1741 gcccgctgcc tgctggaaac cccgggtgtc tgtgacgtcc acgacttgca tctgtggagc
     1801 ctcggcgccg ggagcccgct gttgacggca cacctggtga tggaccactc ggagccggcg
     1861 gaccgcgtgc tgcgggcggc gagcgacgcg ctgctgacgc ggttcggcgt cacgcactcg
     1921 acgctgcaga tcgagccgcc ggactacaac atcgttcagc agctgacgcc ggaggtcgcg
     1981 ccgagcggct cgacggccga cgactgagct acagctcgaa ggtcaccccc tgggccagcg
     2041 gcatctcact gccccagttg atcgtgttcg tctggcgccg catgtaccac ttccacgagt
     2101 ccgagccgga ctcgcgcccg ccgccggtct ccttttcgcc gccgaaggcc ccgccgatct
     2161 cggcgcccga ggtcccgacg ttgacattgg cgatgccgca atcgctgccg gcgtgcgaga
     2221 cgaacgtctc cgccgcgatc acgctggtgg tgaagatcga cgaggacaga ccctgcggca
     2281 cgtcgttgtg gatccggatc gcctgctcca gatcgtcgaa ctcgtgcacg tacaggatcg
     2341 gggcgaacgt ctcgtgtttg acgatgtcgg cgtcgggcga gatcccgacg atcgtcggtt
     2401 ggacgaaatg gccggggcgg tcgatcgtct tgccgccgca gatcaccttg ccgccctgct
     2461 cagcgacctt ggccagcgcc tgctccatgt cgcgtaccgc ggcctcgttg accagcggtc
     2521 ccatcagcgt cgtcttgtcc agcgggtcgc cgatcggaac cgaagcgtag gcccgcacga
     2581 gcttggcggt gaaatccgcg gcgatgccct gttgcaggat caaccgccgc gtcgtcgtgc
     2641 agcgctgtcc ggccgtgccg acggcgccga acaacaccgc acgcagcgcc agttcctggt
     2701 cggcgtcgtc catcactacc accgcgttgt tgccgccgag ctcgagcagc gcacgcccca
     2761 gccgctggcc gacgacctcg ttgatgcggc ggcccatccg gcacgaaccg gtcgccgaga
     2821 tcagcggcaa ccgtacgtcg gccagcatcc gctcgccgac gtcggcaccg cggccaacga
     2881 tcaggttgaa cacgccgctc agaccgttgc gctgcatcac gcggttgcag atgttctgga
     2941 ccgccaccgc ggtgagcggt gtctgcgacg agggtttcca gatcatcgtg tcgccgcaga
     3001 cggcggcgat tgccgcattc caggaccaga cggcgacggg gaagttgaag gcggtgacga
     3061 tgccgaccgg accgagcgga tgccactgct cgtacatgcg atgccgcgag cgctcggaat
     3121 gcatggtcaa gccgtagagc tggcgggaga gaccgaccgc gaagtcgcaa acgtcgatca
     3181 tctcttgtac ttcgcccaga ccctcggtga ggatcttgcc catctcgatc gagaccaaac
     3241 ggccgagcgg ctccttgtgg attcgcaact cctcgccgag ctgccgcacg atctccccgc
     3301 gtctcggcgc gggagtcatc cgccaaccga gaaacgctcg ctgcgccgcc tcagcgacgc
     3361 gctcgtagtc ggccgcggac gcctgacgca cggtcgcgat cggctcctcg ttcgccgggt
     3421 tgtaggagat cagctccgat cctcgggtgt cgaaccactc gccggtgcag gcgccggcgt
     3481 tgaccggctc gattcccagg gcatccagca catcgtcgga catgcggcct ccatccgacc
     3541 ggcggctctt ccgcccctgt cgggcgccgc gcgatccgac gtgcgggcgg ttcgggcttc
     3601 ggttcgggaa ggcgcagtct accagacggg cgcaatcgcg tcccggcgcc ggcgcgtcac
     3661 agcgaagcgg gcggcaacag ataacgcacg tcgtc
//
LOCUS       DASPGN010000529        10080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1269571, whole genome shotgun sequence.
ACCESSION   DASPGN010000529 DASPGN010000000
VERSION     DASPGN010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10080
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269571"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..2836)
                     /locus_tag="VD788_15410"
     CDS             complement(20..2836)
                     /locus_tag="VD788_15410"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HXV77701.1"
                     /translation="MEIIGDDASGTLSTIESHAGITVEDKATAYLQFAAKQDPVEAHN
                     TLNRVLELGLEVQGTDKAQRLLASTYTQLALLHEGEPSRQAYLLGKALEQTEPQAMRQ
                     MLEARIVELGGNDDPATQFAYQPPATTDVSTPRNFGTDDSCTGAPAVGVPSIIPMDIA
                     NPIGSFRDRNFLEVVIPGPLGMALEIETTSPNCNSDATCSATAYDTDMNLWGSCTNNF
                     EGNLVERDINAGDGGVGWLSKIQTDCLLPGSYYLEVKGQFGSAPKDFDVEIRQIGSCV
                     VPVPDSYESDNAISEGADIGFSTSSPTQANAWGRQRSEIQGRSIFPPNDDDHAIIKLN
                     DQTTRVQMGSQVQFGSVWNGGVSIPSGPDEDSQIILFYGEDPHGGICNSAPLVIPNNY
                     CKSSGDCDPDATPADPTQPASTCIPLWQLRVPGEARSRWQPDNALAFNDDVNPASNLG
                     SALDICLPRTQPGGPSLALNGGGFAIRSRGWRPPFPGSVSGLAYDYEVSSRNNGPCLY
                     ESEPNQSFPDATPIVANTPVNGTWEGSETYPLFDVDIWGPFDVQSPGEEVTIEVFPEL
                     LNGLIAKATIELWAYPDDNGNAHQVMTIDTGSAPDARVNLDVQNADDFLGNTMADAGY
                     YVVVSSSTAVPNWYYTLRVITPRVDAVDIEPNDFVAQPVDYRGNTFVVAEISEVCDID
                     RFSFTVPESQFLDMTTTDPGTDTVMSFERVQPDAFEFFVTAPAALAGPYAFGSSAYTG
                     YVPATSDNYNGEVVEANDGTGVTWDACEPLVNAAAISGKVCLVTRGTCAFTQKNLMCQ
                     AAGASATLVRNTAAAPIVMGGDCGGLCEIPQLSVGIADGDLIRANLPGVFVRANNKEF
                     LACDDDGRTFGSNQFLSQITGCVPPGDYIISVRGWNTSTGPYVLNFNGAAGCTPTEPP
                     SMNDTGTGSSQFCPPNFPFARFCQ"
     gene            complement(3241..4143)
                     /locus_tag="VD788_15415"
     CDS             complement(3241..4143)
                     /locus_tag="VD788_15415"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HXV77702.1"
                     /translation="MLRFLSWLSLATVILAAAFPAAVAAEPDTYPEEIDAYRRARLER
                     LTAPDGWLSLIGLHWLEPGKNAVGSDPALAVVLPADVSPPRAGELILADAVVRFVPEP
                     DAAVTLGGAAVPDRPLRDDVTGEPDVLELGRLRMHLIRRGDRFALRVKDPASPARTGF
                     LGIDYFALDRAFRVEARFVPFDEPETIEVPTVTGGTTRMSIPGRLTFELAGRPLSLDP
                     LVSGPDDDEWFLIFRDGTSGRESYGAGRYLYVPITSGPVVIDFNKAYNPPCVFTPYAT
                     CPLPPAANRLAVEIRAGEKTYRGH"
     gene            complement(4148..5152)
                     /locus_tag="VD788_15420"
     CDS             complement(4148..5152)
                     /locus_tag="VD788_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HXV77703.1"
                     /translation="MRDGPLDVVGIGNAIVDVLVRVDDRWLVEHGLKKGTMTLIDDDR
                     AEKLAVSIGKGVECSGGSAANTMAGLASFGGRGAYMGKVRRDRLGAVFASDIRSVGVE
                     YATPAAASGPATGRCLVLVTPDAQRTMQTYLGASGTFGPADLDPDVIHRARITYLEGY
                     LWDPPEAKRAFLRACRIAHEAGRRVALTLSDPFCVDRHRDDLLALIREHVDIVFANRD
                     ELLALFETAAFDEAFSSLRAVCPTAVVTCGAEGSIAADARGVSTIAAVTLSGVVDTTG
                     AGDLYAAGFLFGLARELPLARCGELGSLAAAEIIGHLGARPEVRLAELAASRGLARGG
                     "
     gene            complement(5225..6082)
                     /locus_tag="VD788_15425"
     CDS             complement(5225..6082)
                     /locus_tag="VD788_15425"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HXV77704.1"
                     /translation="MSDHPSRPTTPVPPARSLLYVPAASSRMLAKLETLPADALMLDL
                     EDGVPPDAKATARENLRRVDWAGVRRWMLRINAAGDPHHEADLELAEQLRAPCVVLPK
                     VEDDLVVARTASRAARWGAKIGLMIETPAGLAKVDRLASHEGVEWLVYGSADLRFRLG
                     ARPDPDRGWERPAMFRILLAARVAGCLAIDAVHFRFRDRAGLTRAAEIARQMGFDGKS
                     CIHPGQVATIHEVWAPTADEIAWAEQVVRSWREQQGASRGIVVVDGEMIEALHVRLAE
                     RWLARQGGR"
     gene            complement(6079..6582)
                     /gene="infC"
                     /locus_tag="VD788_15430"
     CDS             complement(6079..6582)
                     /gene="infC"
                     /locus_tag="VD788_15430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXV77705.1"
                     /translation="MRINEQIRLSPVRLIGAGGEQVGIVPIEQARQAAAEAGLDLVEV
                     ASEARPIVVRIMDWGKHRFEANKRAREARKKESRISVKQVKLRPNIDDHDLETKLNHA
                     RRFLAAGDKVKVTIMFRGRDLRRPENGRKLLDRVIEKMDEVAAVEAAPGAIVNRDMSM
                     VLAPRKT"
     gene            complement(6714..7067)
                     /gene="queF"
                     /locus_tag="VD788_15435"
     CDS             complement(6714..7067)
                     /gene="queF"
                     /locus_tag="VD788_15435"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HXV77706.1"
                     /translation="MATRPSKELETFPNPEPAREYEIRFTCAEFTCLCPRTGQPDFAT
                     VTIRYVPDAVCVELKSLKLYLWSFRDEGHFHEAVTNRILDDLVAATSPRRMTVEGDFH
                     IRGGIHTVIRADYPG"
     gene            7167..8462
                     /locus_tag="VD788_15440"
     CDS             7167..8462
                     /locus_tag="VD788_15440"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV77707.1"
                     /translation="MSIPLPGRTLPLVPTGFGALRHRNFRLLWIGQFVSLIGRWMQSV
                     AQGWLVLELSGSAFDLGFVGFCSFSPILLFALVGGAAADRVRRRRALLWTQGTAMVLA
                     LSLALLTALGIVEVWHIAVIAFGVGVTGAFDIPIRQSLLQDLVGRADLPNAIALNSLA
                     FNGARLIGPALAGVLLAAAGEATVFLLNALSYLAVLFGLVRMRAVPGVMRVPGTWLAE
                     MREGVRWVASSAKARVILSLVVISSVFGLPYSILLPVFARDVLDVGSRGLGFLTGATG
                     LGAVIGALYLARQSGDGGRGRVVAAAIVLLGSGLVAFSQSTHFALSLSLLVVVGGAMI
                     VQMASSNTLLQLLAPAELRGRVVGFYMLAFMGMAPIGSLLAGVLASEIGVRATVRIGG
                     LVCLATGGVLAVRLPSSRRGAASATTSARGSRRRTRSGP"
     gene            complement(8413..8781)
                     /locus_tag="VD788_15445"
     CDS             complement(8413..8781)
                     /locus_tag="VD788_15445"
                     /inference="COORDINATES: protein motif:HMM:NF025050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV77708.1"
                     /translation="MKLAHTMIRVKDLDATLAFYTDFIGLHEVRRKEIGSEAMLVFLA
                     DEDEHYQIELTVNHGRTDYDLGDQFGHLAFFTDDLAAVVAEVERRGWSFRRSRPELSS
                     RYIFVKDPNGYDVEILERRS"
     gene            complement(8795..9964)
                     /locus_tag="VD788_15450"
     CDS             complement(8795..9964)
                     /locus_tag="VD788_15450"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXV77709.1"
                     /translation="MRLTRFLILALVFLCGVTLGRYSDPASSERSRPAAAVSPVASPP
                     VTNTRSSLDELGEEERRGIEVYRVASSSVVNVTSTALRRNFFFELLEVPQGAGSGFFW
                     DDEGHLVTNYHVIEGANNFTVTLADQSEWEAELIGIAPEKDLAVLRVAAPADKIIPLP
                     RGRSDRLMVGQRVLAVGNPFGLDQTLTVGVVSALDRELRSPAGRVIRDVIQTDAAINP
                     GNSGGPLLDSTGRVIAVNTAIYSPSGASAGIGFAIPVDTVSRLVPQLIRFGRPIEPGI
                     DGLRWLTDRQAGRFGLSRVGVVVREVDDGSRADRAGLVGIGVDRRGRYVLGDIIVGAD
                     GTEIRTIDEFRDVLERVGVGGSVTLVVERDGRRREVEVELERLDFGAQRDPRRQL"
BASE COUNT         1530 a         3384 c         3473 g         1693 t
ORIGIN      
        1 tgaacgggcc cggccctgac tactgacaga agcgagcgaa cgggaagttc ggcgggcaga
       61 actgcgaaga tcccgtcccg gtgtcgttca tcgacggcgg ctcggtcggc gtgcagccgg
      121 cggccccgtt gaagttgagc acgtagggac cggtgctggt gttccaaccg cggacgctga
      181 tgatgtaatc accgggcggc acgcaaccgg tgatctgcga caagaactgg ttcgaaccga
      241 aggtgcggcc gtcgtcgtcg caggcgagga actctttgtt gttcgcccgg acgaagacac
      301 ccggcaggtt ggcgcggatc aggtcgccgt cggcgatccc gacgctgagc tgcgggatct
      361 cgcacagtcc gccgcagtcg ccacccatta cgatcggcgc cgcggccgta ttacggacca
      421 gcgtcgccga agccccggcc gcctgacaca tcaggttctt ctgggtgaag gcgcaggtgc
      481 cgcgggtgac gagacagact ttgcccgaga tggcggccgc gttgaccagc ggctcgcagg
      541 cgtcccaggt cacgccggtg ccgtcgttcg cctcgaccac ctccccgttg tagttgtcgg
      601 aggtcgcggg cacgtagccc gtgtaagccg acgagccgaa cgcatacgga ccggcgagcg
      661 ccgccggagc ggtgacgaag aactcgaagg cgtccggctg gacgcgctcg aacgacatca
      721 ccgtgtcggt acccgggtcg gtcgtcgtca tgtcgaggaa ctggctctcc ggcacggtga
      781 agctgaagcg gtcgatgtcg cagacctcag agatctcggc gacgacgaag gtgttgccgc
      841 gatagtcgac cggctgggcg acgaagtcgt tcggctcgat atcgacggcg tcgactctcg
      901 gcgtgatcac gcgcagcgtg tagtaccagt tcggcacggc ggtcgacgag ctgacgacga
      961 cgtagtagcc ggcatccgcc atcgtgttgc cgaggaagtc gtcggcgttt tgaacgtcga
     1021 gattgacccg ggcatcgggt gccgagccgg tgtcgatcgt catcacctga tgggcgttgc
     1081 cgttgtcgtc cggataggcc cacagctcga tcgtcgcctt ggcgatcagc ccgttcagca
     1141 gctcggggaa gacctcgatc gtcacctctt cacccggcga ctgcacgtcg aacggccccc
     1201 agatgtcgac gtcgaacaac gggtaggtct ccgacccttc ccaggtcccg ttgaccggcg
     1261 tattcgccac gatcggcgtc gcgtcgggga agctctggtt gggctcggac tcgtagaggc
     1321 acggtccgtt gttgcgcgac gacacctcgt agtcgtaggc cagacccgag acggagcccg
     1381 ggaacggcgg gcgccagccg cggctgcgga tcgcgaaccc accgccgttc agcgcgagcg
     1441 acggcccgcc gggctgtgtg cgcggcagac agatgtcgag cgcggagccc aggttggacg
     1501 cggggttgac gtcgtcgttg aacgccagcg cgttgtcggg ctgccagcgc gagcgggcct
     1561 cgccgggaac gcgcagctgc cacagcggga tgcaggtgga cgcgggttgc gtcggatcgg
     1621 ccggcgtcgc gtcgggatcg cagtcgccgc tgctcttgca gtagttgttc gggatgacga
     1681 gcggggcgga gttgcagatg ccgccgtgcg gatcctcacc gtagaacagg atgatctgcg
     1741 agtcctcgtc gggaccggac gggatgctga caccgccgtt ccacacgctg ccgaactgca
     1801 cttgcgaacc catctgcacg cgggtcgtct gatcgttcag cttgatgatc gcgtggtcgt
     1861 cgtcgttcgg cgggaagatg ctgcggccct ggatctcgct acgctgccgg ccccaggcgt
     1921 tggcctgcgt ggggctcgag gtcgagaaac cgatgtcggc gccttccgaa atggcgttgt
     1981 ccgactcgta gctgtcgggc accggcacca cgcaggagcc gatctgccga atctcgacgt
     2041 cgaagtcctt cggcgccgag ccgaactgcc ccttgacctc gaggtaatag gagccgggca
     2101 gcaggcagtc ggtctggatc ttggacaacc agccgacacc gccgtcgccg gcgttgatgt
     2161 cgcgctcgac gaggttgccc tcgaagttgt tggtgcacga gccccacaga ttcatgtcgg
     2221 tgtcgtaagc cgtcgcggag cacgtggcgt cgctgttgca gttcggcgag gtcgtttcga
     2281 tctcgagcgc cattcccaga ggaccgggga tgacgacctc gaggaagttc cggtcgcgga
     2341 agctgccgat cggattcgcg atgtccatcg ggatgatcga cggtacgccg actgccggtg
     2401 cgccggtgca gctgtcatcg gtgccgaagt tgcgaggcgt gctgacgtcg gtcgtcgcgg
     2461 ggggctggta ggcgaactgc gtcgcggggt cgtcgttccc gccgagctcc acgatccgcg
     2521 cttcgagcat ctggcgcatc gcctggggct cggtctgctc gagggccttg ccgagcaggt
     2581 aagcctgacg gctgggctcg ccttcgtgca gcagggcgag ctgggtgtac gtcgaggcca
     2641 acagccgctg cgccttgtcg gtgccttgca cctcgagacc gagctcgaga acccggttca
     2701 acgtgttgtg cgcttcgacc gggtcctgct tcgctgcgaa ctgcaggtac gccgtcgcct
     2761 tgtcttcgac tgtgattccc gcgtgggatt cgatcgtgga caaggtaccg gaagcgtcgt
     2821 ctccgatgat ctccaccggg attccaggtt gggttcggcg gaactgatca ccgaacgtct
     2881 cctggatgtc gaccggtgcc ccggcgcgcg ggtgcgccgt gcgggccgcg cggtcccggg
     2941 aacgggtccg gtctccggcc gagatcgctg cgaaaacaag ggcgaggcag acagccaggg
     3001 ccgccgccac gaaaatcttt ctgctcatgc tccccccctg ggatgaggac tggtattacc
     3061 ccgctgacgg aggcgttgat gcaacaagcc tctcataact cgcctgaaag ttacgcaagt
     3121 ttgacaaggc gggcaaggac tatttgggac gcgcgacggg cggtgatcgg taccggttgc
     3181 gagacgcgtt ggcgggccgg tttgcgtcgg tctaccacaa attcctatat caacgcaaaa
     3241 tcagtgaccc cggtaagtct tctcgccggc acggatctcg accgcgagcc ggttcgcagc
     3301 cggtggcaac ggacaggtcg cgtacggcgt gaagacacag ggtgggttat acgccttgtt
     3361 gaagtcgatc accaccggtc cggaggtgat cgggacgtac aggtaccgtc ccgcgccgta
     3421 gctttcgcgc ccgctcgtcc cgtcgcggaa gatcaggaac cactcgtcgt cgtccgggcc
     3481 cgacaccaac gggtcgagcg agagcggacg gccggcgagc tcgaaggtga ggcggcccgg
     3541 aatcgacatg cgggtcgtgc cgcctgtgac cgtcgggacc tcgatcgtct cgggctcgtc
     3601 gaacggaacg aagcgggcct cgacgcggaa cgcccggtcg agggcgaagt agtcgattcc
     3661 gaggaatccg gtacgcgcgg ggctcgccgg gtccttgacc cgcagcgcga aacggtcgcc
     3721 gcgccggatg agatgcatgc ggagccgccc gagctcgagg acgtcgggct caccggtgac
     3781 gtcgtcgcgc aggggtcggt ccggaacggc ggcgccgccc agggttaccg cggcgtcggg
     3841 ctcgggaacg aagcgcacga cggcgtctgc gagtatcagc tcaccggccc tgggcggcga
     3901 gacgtccgcc ggcaagacga ccgccagcgc cgggtcggag ccgaccgcgt tcttccccgg
     3961 ctcgagccag tgcagcccga tcagggacaa ccacccgtcg ggcgcggtca gtcgctcgag
     4021 ccgcgctcgg cggtaggcgt cgatctcctc ggggtaggtg tcgggctcgg cggccaccgc
     4081 cgcgggaaag gcggccgcca gtatcaccgt tgcgagcgag agccacgaca ggaagcggag
     4141 catcgtttca tcctcctcga gccagaccgc gggacgcggc cagctcggcc aaccggacct
     4201 cgggacgcgc tccgaggtgt ccgatgatct ccgccgcggc caggctgccg agctccccgc
     4261 agcgggcgag tggcagttcc cgtgccaaac cgaagaggaa ccccgccgcg tacaggtctc
     4321 cggcgcccgt cgtatcgacg actcccgaca gcgtgaccgc cgcgatcgtc gacacaccgc
     4381 gcgcgtccgc cgcgatcgac ccctcggcac cgcaggtgac gacggcggtc gggcagacgg
     4441 cccgcagcga cgagaacgcc tcgtcgaacg ccgccgtttc gaacaacgcc agcagctcgt
     4501 cccggttcgc gaagacgatg tcgacgtgct cgcggatcaa cgcgagcagg tcgtcgcgat
     4561 gccggtcgac gcagaacggg tcggacaggg tgagagccac ccgtcggccg gcctcgtggg
     4621 cgatgcgaca ggcccgcagg aaggcccgct tggcttcagg cggatcccac aaatatccct
     4681 cgaggtaggt gatgcgggct cggtggatga cgtccgggtc gaggtcggcc ggaccgaagg
     4741 tcccgctggc gccgagatag gtctgcatcg tccgctgggc gtcgggtgtc accaggacga
     4801 ggcagcgtcc cgtcgccggc ccactcgcgg cggccggagt ggcgtactcg actccgaccg
     4861 agcggatgtc gctcgcgaag acggcgccga gccgatcgcg acgcaccttg cccatgtacg
     4921 cgccgcggcc gccgaacgaa gccagcccgg ccatcgtgtt ggcggcggag ccgcccgagc
     4981 actcgacccc ctttccgatc gagaccgcga gcttctcggc gcggtcgtcg tcgatcaggg
     5041 tcatcgtgcc cttcttcaga ccgtgctcga cgagccaccg gtcgtcgacc cggaccagca
     5101 cgtcgacgat cgcgttgccg atcccgacga cgtcgagcgg accgtctcgc attcctcgac
     5161 actcccgatc gctgccggtg cggtcccgag accgaccggc ggaccctcat tattaccgga
     5221 tcgcctacct tccgccctgc cgggccagcc agcgctcggc cagccggacg tgcagcgcct
     5281 cgatcatctc gccgtcgacc accacgatgc cgcgcgatgc gccctgctgc tcgcgccagg
     5341 agcggacgac ctgctcggcc caggcgatct cgtcggccgt cggcgcccat acctcgtgga
     5401 tcgtcgcgac ctgccccgga tggatgcagc tcttcccgtc gaaccccatc tggcgcgcga
     5461 tctccgcggc ccgggtcaac ccggcgcgat ctcgaaaccg gaagtggacg gcgtcgatcg
     5521 ccaggcagcc cgcgacccgc gccgcgagca ggatgcggaa catcgccggc cgttcccacc
     5581 ctcgatcggg atccggccga gcgccgaggc ggaaccgcag atcggccgac ccgtagacga
     5641 gccattcgac accctcgtgg gaggcgagcc ggtcgacttt cgcgagcccc gccggcgtct
     5701 cgatcatcaa cccgatcttc gcgccccagc gcgctgcccg gctcgccgtg cgggcgacga
     5761 cgaggtcgtc ttcgaccttg ggcagcacga cgcacggagc gcgcagctgc tcggccagct
     5821 cgagatcggc ctcgtggtgg ggatcgcccg cggcgttgat ccgcagcatc cagcgacgga
     5881 cgccggccca gtcgacgcgg cgtagattct cccgcgccgt cgccttggca tcgggcggca
     5941 cgccatcctc gaggtcgagc atcagcgcgt cggccggcag cgtttcgagc ttggccagca
     6001 tccgcgagct cgccgccgga acgtagagca ggctgcgggc tggcgggacg ggcgtagtcg
     6061 gtcgggaggg atggtcgctc aagtcttgcg gggcgcgagc accatcgaca tgtctcggtt
     6121 cacgatcgcc ccgggcgctg cctcgaccgc ggcgacttcg tccatcttct cgatcacgcg
     6181 atcgagcagc ttgcggccgt tttccggccg cctcaggtcg cgtccgcgga acatgatcgt
     6241 gaccttcacc ttgtcgcccg ctgcgaggaa tcgtctggcg tgattgagct tggtctcgag
     6301 gtcgtggtcg tcgatgttcg gtctgagttt gacctgcttg accgaaatcc tcgattcttt
     6361 cttacgcgcc tcgcgggcgc gtttgttggc ctcgaagcga tgcttgcccc agtccatgat
     6421 gcgcaccacg atcgggcgcg cctcggaggc gacctcgacc aggtccaggc cggcctcggc
     6481 ggcggcctga cgggcttgtt cgatggggac gatgccgact tgctctccgc cggcaccgat
     6541 caagcggacg ggactgagac ggatctgctc gttgatacgc accttggttt cgataagacg
     6601 attcctcctt tgtctttagc gcgacctgaa cttgccggcg gaatcccgtg cccgggacgc
     6661 caacgcgtgg gaatataaca cccgagccgc cgggggccaa gatttcgatc ggctcaaccc
     6721 gggtagtccg cgcggatcac cgtgtggatc ccaccgcgga tgtggaagtc gccctcgacc
     6781 gtcatccggc gcggactcgt ggcggcgacc aggtcgtcga ggatccggtt ggtcaccgcc
     6841 tcgtggaagt gcccctcgtc gcggaacgac cacaggtaga gcttgagcga cttcagctcg
     6901 acgcagaccg cgtcgggcac gtagcggatc gtcaccgtcg cgaagtccgg ctggccggtc
     6961 cgcgggcaca gacaggtgaa ctcggcgcag gtgaagcgga tctcgtactc gcgggcaggc
     7021 tcggggttcg ggaaggtctc gagctccttc gacggtcggg tcgccacggt tcgctcctgt
     7081 cgccggcact ccggcacgca gggcccatcg taccgggtcg gtcgcccggt caccacgggg
     7141 tcatccacag ccgatacaat ccgtgaatgt cgatcccgtt acccggtcgg accctgccgc
     7201 tcgtgcccac cggcttcggg gcactccgcc accggaactt ccgtttgctg tggatcggcc
     7261 agttcgtctc gttgatcggc cgatggatgc aatcggtcgc ccagggatgg ctggtgctcg
     7321 agctgagcgg ctcggcattc gatctgggtt tcgtgggctt ttgcagtttc tcgccgatcc
     7381 tgctcttcgc actggtcggg ggagcggccg ccgaccgcgt gcggcgacgg cgagcgctgc
     7441 tgtggacgca ggggacggcg atggtgctcg ccttgtcact cgcgctgttg accgcgctcg
     7501 gcatcgtcga ggtgtggcac atcgcggtca tcgccttcgg cgtcggagtg accggggcgt
     7561 tcgacatccc gattcgccag tcgctgctcc aggatctcgt cggccgcgcc gatctcccca
     7621 acgccatcgc gctcaactcc ctcgcgttca acggcgcccg gttgatcggt ccggcgctgg
     7681 ccggggtgct gctggcggcg gcgggcgagg cgacggtctt cctgctgaac gcactctcct
     7741 acctcgccgt gctgttcggg ctcgtgcgca tgcgcgccgt accgggggtc atgcgggttc
     7801 ccggcacgtg gctcgccgag atgcgggagg gcgttcgatg ggtcgcgtcg agcgcgaagg
     7861 cgcgcgtgat cctgtcgctc gtcgtgatct cgagcgtctt cggactgccc tactcgatcc
     7921 tgctgcccgt cttcgcccgc gacgtgctcg acgtcggctc ccgcggtctg ggcttcctca
     7981 ccggggcgac cggtctcggc gccgtgatcg gtgcgctgta cctcgcgcgc caaagcggcg
     8041 acggcggtcg cgggcgtgtc gtggcggccg cgatcgtcct gctcgggtcg ggtctcgtcg
     8101 ccttctcgca gtcgacccat ttcgccttgt ccctgtcgct gttggtcgtc gtcggcggcg
     8161 cgatgatcgt ccagatggcg tcgagcaaca cgttgctcca gctgctggcg ccggccgagc
     8221 tccgcggccg cgtcgtcggc ttctacatgc tggccttcat ggggatggcg ccgatcggct
     8281 cgctcttggc cggcgtgctc gcgagcgaga tcggggtccg cgcgaccgtg cggatcggcg
     8341 ggctggtctg tctggccacg ggcggcgtgt tggccgtccg gctgccgtcg tcgaggcgcg
     8401 gggcggcgtc cgctacgacc tccgctcgag gatctcgacg tcgtacccgt tcgggtcctt
     8461 gacgaagata tacctcgagc tgagctcggg tcgggagcgc cggaagctcc acccgcgccg
     8521 ttcgacctcc gccacgaccg cggccagatc gtcggtgaag aacgcgagat gaccgaactg
     8581 gtcgccgaga tcgtagtccg tccgaccatg attgacggtc agctcgatct ggtagtgctc
     8641 gtcctcgtcc gcgagaaaga cgagcatcgc ttccgagccg atctccttgc gccgcacttc
     8701 gtgcagaccg atgaaatcgg tgtagaacgc gagcgtcgcg tccaagtcct tcacgcggat
     8761 catcgtatgg gccagtttca cgatccgttc ctcctcacag ctgtcgtcgc gggtcgcgtt
     8821 gcgcgccgaa gtcgagccgc tcgagctcga cctcgacctc gcgccgccgc ccgtcgcgct
     8881 cgacgacgag ggtcacgctc ccgccgacgc cgacgcgctc gaggacgtcc cgaaactcgt
     8941 cgatcgtccg gatctcggtc ccgtcggcac cgacgatgat atcgccgaga acgtaccgcc
     9001 cgcgccgatc gacgccgatg ccgacgagac ccgcgcgatc ggcgcggctg ccgtcatcga
     9061 cctcgcgaac gaccacgccg acccggctga gaccgaagcg gccggcctgc cggtcggtca
     9121 gccagcgcag cccgtcgatg cccggctcga tcggccttcc gaagcggatc agctgcggca
     9181 cgagtcggct gaccgtgtcg accggaatcg cgaagccgat gccggccgac gcgccgctcg
     9241 ggctgtagat cgcggtgttg acggcgatca cccggccggt cgaatcgagc agcgggccgc
     9301 ccgagttacc ggggttgatc gcggcgtcgg tctggatcac gtcgcggatc acccggccag
     9361 ccggcgatct cagttcacgg tcgagcgcgc tgaccacgcc gaccgtcagc gtctggtcga
     9421 gcccgaacgg atttccgacc gccagcaccc gctgcccgac catcagccga tcggaccgcc
     9481 ccctgggcag cgggatgatc ttgtcggcgg gtgccgcgac gcgcaggacc gccaggtcct
     9541 tttccggtgc gattccgatc agctcggcct cccactcgct ctgatccgcc agcgtcacgg
     9601 tgaagttgtt ggccccttcg atgacgtggt agttcgtgac gaggtgccct tcgtcgtccc
     9661 agaagaagcc gcttccggcc ccctgcggca cctcgagcag ctcgaagaag aaattgcggc
     9721 gcaacgcggt cgaagtgacg ttcacgacgg acgacgacgc gacgcggtag acctcgatcc
     9781 cgcgccgttc ttcctcaccc agctcgtcga gcgacgaccg cgtgttcgtc accggcggag
     9841 aggcgaccgg ggagaccgct gccgccggcc gcgaacgctc cgaggaggcg gggtcggagt
     9901 agcggccgag cgtgaccccg cagaggaaga cgagtgccaa gatcaagaaa cgggtcagcc
     9961 tcatcgtcgg cctccaccgc gtcggcgcca cggggagcgt gacgcgcgac ggcgagtcga
    10021 tccgcgatca ggctagcatc ggccgaggtc gacgagcgag aacgggggcg atcgaaaaga
//
LOCUS       DASPGN010000530         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_450834, whole genome shotgun sequence.
ACCESSION   DASPGN010000530 DASPGN010000000
VERSION     DASPGN010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450834"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..617
                     /locus_tag="VD788_15455"
     CDS             21..617
                     /locus_tag="VD788_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77710.1"
                     /translation="MPLDTQPGTTVTVRNLTCIPSNSDVNTHGDSEGVLVRQGGQLVL
                     SNSIVYAGYGTAISGFASNECLEVESAVSLDFAAANDAFTQTGNVLACEEAVKGTLAN
                     GDALTEWFLGANPSTNGADFSFNAGNFTIIDPANANAQVLQAGTFFTAPAIVDEAAVT
                     QVADASGLGAVQSSDDWTSPWAFGLRSANADVPLWFAP"
     gene            639..>2830
                     /locus_tag="VD788_15460"
     CDS             639..>2830
                     /locus_tag="VD788_15460"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXV77711.1"
                     /translation="MNRFPLRDAVRLALLGLSTLATAPSLGQEAAEGQAEPTVLEDIE
                     VVGRQRSAADDILQERIDAAVVQDLVNIEQITRVGDSTVSAALRRLPGVTLVADQFIY
                     IRGLGERYSSTTVNGAFVPSPDLTRNVIPMDLFPAEIVESISIQKGFTADQPAAFGGG
                     NVDVRTRAIPEDFLFNVSLGIGTNSGSSDDGLTYAGGSDDRLGDDDGTRALPRAIDQA
                     IRTYRGNLSQTNIFNTLLMDGQDHTFAEAQGINRELAVSLNRDVDFRDKSMEPDKSIE
                     ISAGNSWFFGDAEQWKFGVIGVADYNSNWRNRERTVRSVTEPTLDFDEQFRTVNQTVL
                     TGYFGAGIDYADEHRIEATGIFLRNTEDEASLTLGQNFNFRRENGDRLRNYRIRFEQR
                     ELELLQVRGSHALGDATLNKFDWAPIGFAEGLVVDWYYSDAEATTDIPSEITFSARDR
                     IDPASGELLSTSLRSSGSAAEYRFTDLQDTATSYGWQFTMPYQAGSKAIEISGGYDYY
                     EKGRGYLQKQLQFGTTASAALPILVGTPGQVFTDEHILDPVNEFSLSVGGIGTESYLA
                     GETVDAVWGNFDVNFNEAFRVTAGLRWEDFSRLAVPIDPLEFDVGTGIISIPAEELPN
                     FATTEDGFYPSLAFTWMKPGFRAGDFQLRLGWSETVARPDLREISDATYIDPLTEARV
                     RGNPLLDNSDLSNLDLRAEWFFAGGDNLTVSLFYKDITAPIETVEAAGT"
BASE COUNT          490 a          962 c          951 g          427 t
ORIGIN      
        1 agtcgcgcag cacggcgggc gtgccgctcg acacgcagcc gggcacgacg gtgacggtgc
       61 gcaacctcac ctgcatcccc tcgaactccg acgtcaacac gcacggcgac tccgagggcg
      121 tgcttgtgcg ccagggcggg cagctggtgc tgagcaacag catcgtctac gcgggctacg
      181 gcaccgcgat cagcggcttc gcgtcgaacg agtgcctcga ggtcgagagc gcggtttcgc
      241 tcgatttcgc agcggcgaac gacgcgttca cgcagaccgg caacgtcctc gcgtgcgagg
      301 aggcggtcaa gggcaccctg gcgaacggcg acgcgctcac cgagtggttc ctcggcgcaa
      361 acccgagcac caacggggcc gacttctcgt tcaacgcggg caacttcacg atcatcgacc
      421 cggccaacgc caacgcgcag gtgcttcagg ccggcacctt cttcaccgcc ccggcgatcg
      481 tcgacgaggc ggcggtgacg caggtggccg acgcatccgg gctcggcgcc gtgcagtcaa
      541 gcgatgactg gacgtcgccc tgggcgttcg gtctgcggtc ggcgaatgcc gacgtgccgc
      601 tctggttcgc gccctgaact gacgggggag gtccggcgat gaacaggttt ccactgcgtg
      661 atgcggtgcg gctcgcgctg ctcggattgt cgacgctggc gacggcaccg agcctcggcc
      721 aggaggccgc ggaaggccag gccgagccga cggttctgga ggacatcgag gtcgtcgggc
      781 gccagcgctc ggccgccgac gacatcctgc aggagcggat cgacgcggcg gtggtccagg
      841 acctggtcaa catcgagcag atcacccggg tcggcgacag cacggtgtcc gctgcgctgc
      901 ggcgcctgcc tggcgtgacc ctggtcgccg accagttcat ctatatccgt ggcctcggcg
      961 agcgctattc gagcaccacg gtcaacggcg ccttcgtgcc gtcgccggac ctgacgcgca
     1021 acgtcattcc catggacctg ttcccggccg agatcgtcga atcgatctcg atccagaagg
     1081 ggttcaccgc ggaccagccg gccgccttcg gcggcggcaa cgtcgacgtc cgcacccggg
     1141 caatcccgga ggatttcctg ttcaacgtct cgctcggcat cggcaccaac tccgggagtt
     1201 cggacgacgg ccttacctac gcgggcggca gcgacgaccg gctcggcgac gacgacggca
     1261 cgcgcgccct gccgcgcgcg atcgaccagg cgatccggac ctatcgcggc aacctgagcc
     1321 agacgaacat cttcaacacg ctgctgatgg acgggcagga ccatacgttt gcggaggcgc
     1381 agggaatcaa ccgcgagctc gcggtcagcc tcaatcgcga cgtcgacttc cgcgacaagt
     1441 cgatggaacc ggacaagtcg atcgagatct cggccggcaa cagctggttc ttcggcgacg
     1501 cggagcagtg gaagttcggc gtgatcggcg tcgccgacta caacagcaac tggcgcaacc
     1561 gcgagcgcac ggtgcgctcg gtcaccgagc caacgctcga cttcgacgag cagttccgca
     1621 ccgtcaacca gaccgtgctg accggctact tcggagccgg catcgactac gcggacgagc
     1681 accggatcga ggcgaccggg atcttcctgc gcaacacgga agacgaggct tcgctgacgc
     1741 tgggccagaa cttcaacttc cggcgcgaga acggcgaccg cctgcgcaat tatcgcatcc
     1801 gcttcgagca gcgcgaactg gagctgttgc aggtgcgagg ctcgcacgcg ctcggcgacg
     1861 cgacgctcaa caagttcgac tgggcgccga tcggcttcgc cgagggcctg gtggtggact
     1921 ggtactactc cgatgcggag gcgaccacgg acatcccgag cgagatcacg ttctcggccc
     1981 gcgaccggat cgacccggca tcgggcgagc tgctctcgac ctcgttgcgc tcctccgggt
     2041 ctgcagcgga gtaccgcttc acggacctgc aggacaccgc gaccagctac ggctggcagt
     2101 tcacgatgcc gtaccaggcc ggctcgaagg cgatcgagat cagcggcggc tacgactact
     2161 acgagaaggg gcggggctac ctgcagaagc agctccagtt cggcacgacg gcatcggctg
     2221 cgctgccgat cctggtcggc acgccgggcc aggtgttcac cgacgagcac atcctcgacc
     2281 cggtcaacga gttctcgctg tcggtcggcg gcatcggcac cgaaagctac ctcgcgggcg
     2341 agaccgtgga tgccgtctgg ggcaacttcg acgtcaactt caacgaggcc ttccgggtga
     2401 ccgcgggact gcgctgggag gatttctcgc gcctcgcagt gccgatcgac ccgctggagt
     2461 tcgacgtcgg caccggcatc atctccatcc cggccgaaga gctgccgaac ttcgcgacga
     2521 ccgaggacgg gttctatccg tcgctcgcgt tcacgtggat gaagcccggc ttccgggccg
     2581 gggacttcca gttgcggctc ggctggagcg agacggtcgc gcggccggac ctgcgcgaaa
     2641 tctcggacgc cacctacatc gacccgctga ccgaggcccg cgtgcgcggg aacccgctgc
     2701 tagacaactc ggacctgtcg aacctcgacc tgcgcgccga gtggttcttc gcgggcgggg
     2761 acaacctgac cgtgtcgctg ttctacaagg acatcaccgc gccgatcgag acggtggagg
     2821 cggcggggac
//
LOCUS       DASPGN010000531         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1622201, whole genome shotgun sequence.
ACCESSION   DASPGN010000531 DASPGN010000000
VERSION     DASPGN010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1622201"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VD788_15465"
     CDS             complement(<1..821)
                     /locus_tag="VD788_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HXV77712.1"
                     /translation="MKQILFVALTALAAVLTAPAAEAAPTVYIPLGSGNKVIAVDAAR
                     DRITHSYDGVQNPHGLVTTPDGEYLIVGSLTETPIPQGAPAETPNSLLYVVHPAHGHV
                     MSTIPVSGWTHHQAITPDGKYVISTHPTRGGISVVDVAENRVVRTVKTGSAPNYSLVT
                     KNGKRIFVSNSGNGTISEIDTATWAVARTLEGGPAPEHLVIDEDRGRIYAASGRTGQV
                     SAISIGSGKVERSYDLGKRLHGLDRGDDGRTLFVTVISDDRLVALDPDTGAIRSRL"
     gene            complement(848..1162)
                     /locus_tag="VD788_15470"
     CDS             complement(848..1162)
                     /locus_tag="VD788_15470"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXV77713.1"
                     /translation="MLVAAALSSIALPVLGEASGRDIYVDTCIACHGPDGQGALPGVP
                     DLVALDWRAGGDDATLLRRVREGFRRPGSPLAMPPKGGNPALTDADLRAVLDYMKNML
                     AR"
     gene            complement(1213..1821)
                     /gene="petA"
                     /locus_tag="VD788_15475"
     CDS             complement(1213..1821)
                     /gene="petA"
                     /locus_tag="VD788_15475"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HXV77714.1"
                     /translation="MADAKAFDDRPDPARRKLLVGATGAVGGAARAAASYPFIASLGP
                     SERAKSAGAPVEFDIAPLAPGRLHTLEWRGKPVWVLRRTPEMLAGLKAVEKLLSDPHS
                     EVSSQQPEYARNVGRSINEETFVTVALCTHLGCIPSYYPEPGSIQPGWPGGFYCPCHG
                     SKFDLAGRVFKGSPAPTNLEIPPYRYEGTSRLVVGADASGEA"
     gene            complement(1891..2316)
                     /locus_tag="VD788_15480"
     CDS             complement(1891..2316)
                     /locus_tag="VD788_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77715.1"
                     /translation="MAIAVLASAATVMTTRSAGAEAQWMWRDVHEYRVALAVESVHDP
                     AADDPRHAPAFDHRFTVIVSASGDRAPVEVESVSLDVAVRSYSGTVLPLARASGEGKS
                     VFEGNVRMARGQTYRILVHFRPAGSPMTREAQYEYRHHH"
BASE COUNT          368 a          806 c          777 g          391 t
ORIGIN      
        1 agccgcgagc ggatcgcgcc ggtgtctgga tcgagcgcga ccagccggtc gtccgagatc
       61 accgtgacaa acagtgtcct gccgtcgtcg ccgcgatcga gcccgtgcag ccgcttgccg
      121 aggtcgtagg agcgctcgac cttcccgctc ccgatggaga tcgcgctcac ctggccggtg
      181 cgcccgcttg cggcatagat gcggccgcgg tcctcgtcga tgacgagatg ctccggagct
      241 gggccgccct cgagcgtacg cgccaccgcc caggtcgcgg tatcgatctc gctgatcgtc
      301 ccgttgccgc tgttgctgac gaagatgcgc ttgccgtttt tcgtgacgag ggaatagttc
      361 ggcgcagacc ccgtcttcac cgtccgcacg acgcgattct cggcgacgtc gacgacgctt
      421 atgccgccgc gcgtcgggtg ggtcgagatc acgtatttgc cgtccggcgt gatcgcctgg
      481 tggtgcgtcc atccggagac cggaatcgtc gacatgacgt gcccgtgtgc cggatgcacc
      541 acgtagagca agctgttcgg cgtctcggcg ggcgcgccct gagggatcgg cgtctcggtc
      601 aggctgccga cgatcaggta ctcgccgtcg ggtgtcgtca ccaggccatg cgggttctgc
      661 acgccgtcgt agctgtgggt gatgcggtcg cgcgcggcgt cgacggcgat gaccttgttg
      721 ccgctgccca gcgggatgta gacggtcggc gccgcctctg ccgcaggcgc agtgagcacg
      781 gcagctagtg ccgtgagcgc tacaaacaga atctgcttca tgatgactcc tcgtgtgaaa
      841 taggacatca gcgcgcgagc atgttcttca tgtagtcgag tacggcccgc agatctgcat
      901 ccgtcagcgc cgggttgccg cccttgggcg gcatcgcgag cggggatccg ggcctgcgaa
      961 atccctcgcg aacccgcctc agcagcgtcg catcgtcgcc tcccgcccgc cagtcgagcg
     1021 ctacgagatc cggcacgccg ggcagcgcac cctgaccgtc gggcccgtgg catgcgatgc
     1081 aggtgtcgac atatatgtcg cgccctgagg cctccccaag aaccggcagc gcgatgctcg
     1141 acagcgccgc cgcgaccagc aatctgaccg cgagcgactt cgcgattcgc gttggtggac
     1201 gcctcgtcat tctcacgctt ccccggacgc gtcggcgccg acgaccagcc tgctcgttcc
     1261 ctcgtagcga tagggcggga tctcgagatt ggtcggcgca ggagagccct tgaacacgcg
     1321 ccccgcgaga tcgaacttcg aaccgtggca ggggcagtag aaaccgcccg gccagccggg
     1381 ctggatcgaa cccggctcgg ggtaatagct cggaatgcag cccaggtgcg tgcacagcgc
     1441 gacggtgacg aacgtctcct cgttgatcga gcggccaacg ttgcgcgcgt attcgggctg
     1501 ctgcgaactc acctccgagt gcgggtcgga caggagcttc tccaccgcct tcaggcccgc
     1561 cagcatctcg ggggtgcgcc gaagcaccca gaccggcttg ccccgccact cgagcgtatg
     1621 cagacgtcct ggcgcgaggg gcgcaatgtc gaattcgacc ggcgcaccgg cagacttcgc
     1681 gcgctcgctc ggcccgaggc tcgcgatgaa cggatagctc gccgcggcgc gcgcggcgcc
     1741 acccacggcg ccggtcgcac ccaccagcag cttgcggcgc gcgggatcgg gtctgtcgtc
     1801 aaacgctttt gcgtcggcca ttgccgtctc ccagttcagg gaacctgccg ccgctcagcg
     1861 aagcaccctg cgcgcgatgc gacggccgga tcaatgatgg tgccggtact catactgtgc
     1921 ctcgcgcgtc atcggggaac cggcggggcg gaagtggacg agaatgcggt acgtctgtcc
     1981 acgtgccatg cgcacgttcc cctcgaacac cgatttgcct tctccggatg cgcgcgcgag
     2041 cggcagcacg gttccgctgt agctgcgtac cgcgacgtcc agcgagaccg actcgacctc
     2101 gacaggcgca cgatcgcccg acgcggatac gatcaccgta aaccgatggt cgaacgccgg
     2161 cgcatgtctc ggatcgtccg cggcgggatc gtgtacggat tcgacggcca atgccacgcg
     2221 atactcgtgc acgtcgcgcc acatccactg tgcctctgca cccgcgctgc gagtggtcat
     2281 caccgtcgcc gcggacgcga gcacggcgat cgccaccgac accgccacga ggccagggcg
     2341 gt
//
LOCUS       DASPGN010000532         4088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1385898, whole genome shotgun sequence.
ACCESSION   DASPGN010000532 DASPGN010000000
VERSION     DASPGN010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1385898"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /gene="rny"
                     /locus_tag="VD788_15485"
     CDS             <1..1452
                     /gene="rny"
                     /locus_tag="VD788_15485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HXV77716.1"
                     /translation="VVKSRLRANALLKDAETEAENKKKEILVTAQERLLAMQDESDQR
                     ERELDERETTLQARAGELDRRASDVGRERSEIERRVGELRRAEAETQRHLAASREKHD
                     EAQRTLERITGLTLEQAKQELIAGIEEETRALAARLTRKIEDEARQTAERRAVNLMLQ
                     SSERIDIRDVVESTVSFIELPSDEMKGRIIGREGRNIRALEMATGIDLIVDDTPRSIL
                     ISSFDPLRREIARVAIDRLVEDGRIHPARIEEVVARVREEVDELIQERGNETVLALGI
                     SDLHPRLVHLVGKMRYRTCHGHNLLQHCRETALIAGHMAQELNGRVDVAVRAGLLHEV
                     GRVEQNLNGNPTLYAAELCARFGESPRVVEAIRGLHAEVETKGLEPLLVRVASRMSDA
                     RPGARKENLAVFVERLRRLEEIATRFPGIDRAFCVKAGKEIRVIVNAAQVSDEKIHSL
                     SREIARAVERELAFPGQIKVHVIRETRSVRYAL"
     gene            1463..2350
                     /locus_tag="VD788_15490"
     CDS             1463..2350
                     /locus_tag="VD788_15490"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HXV77717.1"
                     /translation="MDLDHYLKTRKAQVDETLDALLADRDGPAATVVAAMRYAVRAGG
                     KRLRPVLALAAGEACGAPLASLLRPACALELVHTYSLIHDDLPAMDDDEMRRGQPTVH
                     RAFGEAEAILAGDALQSLAFEVLATYPEGEAHAAGRAEAVRVVAHCAGIDGMVGGQVA
                     DLEAERAVVDAEGLDWIHRHKTGALLTASALLGAIHSPAGPAAREALGTYGRNLGLAF
                     QITDDILDRTSTADALGKTPGKDQRAGKATYPRLYGLEESRRRAAELIERALDALRSR
                     GLLSEPLSALARYAVTRDR"
     gene            2347..3186
                     /locus_tag="VD788_15495"
     CDS             2347..3186
                     /locus_tag="VD788_15495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HXV77718.1"
                     /translation="MTPRPPRAERERLDVMLVDRGLASTRARARALILAGRVRCGTDR
                     LTKPGQRYPIDVELTVDRGRRWVGRGAYKLDAGLREFAVDLRDRDCLDVGASTGGFTE
                     VMLLRGAHRVIALDVGRGQLDWRLRRDDRVSVIEGVNARRLRADDLPFAPTFAAIDVS
                     FISIGLVLPAIVAALDRARAGEIVALVKPQFEVGRKRVGRGGIVRDPVLHDEVLRRTI
                     DLAERAGCGATGLVASPIAGAEGNREFLLHLERGRPSLDTAALDRAVEAALVFPLRAE
                     DEG"
     gene            3183..4067
                     /locus_tag="VD788_15500"
     CDS             3183..4067
                     /locus_tag="VD788_15500"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HXV77719.1"
                     /translation="MNRVAPSHTIRRVVIVAKLGPSPEGARLAERIGEWLEGRGIEPG
                     FERNTARAIGRERGVAGDELSSEVDLVIVAGGDGTLLSVARVAAPVGIPILPVNLGSL
                     GFLTELQPGEIFDGLEAVLAGHYAVEERQMLRVRPVLGGKAQPEHALLNDVVIAKSAL
                     ARMISIEVRVDDMPVATYTSDGLIIATPTGSTAYNLSAGGPILDPRMSAFVISPICPH
                     SMSYRPLVVPGGVTVEAILRSDGEAVYLTLDGQIGFPMEVDDALTVDAHPNPVRLVRI
                     GDRGFFEVLRRKLHWGER"
BASE COUNT          641 a         1398 c         1446 g          603 t
ORIGIN      
        1 gtcgtcaagt ctcgcctgcg ggcaaacgcc ctgctgaaag acgcggaaac cgaggccgag
       61 aacaagaaga aggaaatcct ggtaaccgcc caagagcggt tgctcgccat gcaggacgaa
      121 tcggaccagc gggaacggga gctggacgaa cgggagacga cactccaggc gcgggccggc
      181 gagctcgacc ggcgcgcgtc cgacgtcggg cgcgagcgct ccgagatcga gcggcgcgtc
      241 ggcgaactac gccgcgccga agcggagacc cagcgccacc tcgccgcctc ccgcgagaag
      301 cacgacgagg cgcagcggac cctcgagcga atcaccgggc tgacgctcga gcaggcgaag
      361 caggagctga tcgccggcat cgaggaggaa acccgggcgc tcgccgcccg cctgacccgc
      421 aagatcgagg acgaagcgcg ccagaccgcc gagcggcgcg cggtcaacct gatgctgcaa
      481 tcctccgagc gaatcgatat ccgcgacgtg gtcgagtcga cggtcagctt catcgagctt
      541 ccgagcgacg aaatgaaggg tcgcatcatc ggccgcgagg ggcgcaacat ccgcgcgttg
      601 gagatggcga ccggcatcga cctgatcgtc gacgacacgc cgcgctcgat cctgatctcg
      661 tccttcgatc cgttgcgccg cgagatcgcg cgggtcgcga tcgaccgctt ggtcgaggac
      721 gggcggatcc acccggcccg catcgaggag gtcgtcgctc gcgtgcggga ggaggtcgac
      781 gagctgatac aagaacgcgg caacgagacg gtcctcgcac tcgggatctc cgaccttcac
      841 ccgcgtttgg tgcacctggt cggcaagatg cgctatcgca cgtgccacgg tcacaatctg
      901 ctgcagcact gccgcgagac cgcgctcatc gcagggcaca tggcgcagga gctcaacggt
      961 cgcgtcgacg tggccgtacg ggcgggcctg ctccacgagg ttggccgcgt cgagcagaat
     1021 ctcaacggaa acccgacgct ctacgcggcc gagctgtgcg ctcgcttcgg tgagtctccg
     1081 cgcgtggtcg aggcgattcg cggccttcac gcggaggtcg agacgaaggg gctcgagccg
     1141 ctgttggtgc gtgtcgcgag cagaatgagc gacgctcgac ccggtgcacg gaaggagaat
     1201 ctcgccgtct tcgtcgagcg gctgcgacgt ctggaggaga tcgccacgcg ctttcccgga
     1261 atcgatcgcg cgttttgcgt caaggcgggc aaggagatcc gcgtgatcgt caatgccgcg
     1321 caggtctccg acgagaagat ccactctctg tcgcgcgaga tcgcccgcgc cgtcgagcgg
     1381 gagttggcct ttcccggcca gatcaaggtc cacgtcattc gcgagacgcg ttccgtgcgc
     1441 tacgcgctgt gagagacccg gattggacct cgaccactat ctgaagactc gcaaggcgca
     1501 ggtcgacgag acgctcgacg ccctgctggc cgaccgcgac ggtccggcgg cgaccgtcgt
     1561 cgcggcgatg cgctacgcgg tcagggccgg agggaagcgg ttgcggccgg tgttggcctt
     1621 ggccgccggt gaggcgtgcg gcgctccgct cgcttcgctg ctccgtccgg cctgcgcact
     1681 cgagctggtc cacacctact cgctgatcca cgacgatctc ccggcaatgg acgacgacga
     1741 gatgcggcgg gggcaaccga cggtccaccg tgccttcggc gaggcggagg cgatcctggc
     1801 cggcgacgcc ttgcagtcgc tggcgttcga ggtgctcgcg acctaccccg agggtgaggc
     1861 ccacgccgcc ggtcgagccg aggcggtccg cgtcgtggcg cactgcgccg ggatcgacgg
     1921 catggtcggc ggacaggtcg cggacctcga ggccgaacgg gcggtggtcg acgccgaggg
     1981 tctcgactgg atccaccgcc acaagacggg ggcgctgctg acggcgagtg ccctgctcgg
     2041 cgcgatccac tcgccggccg ggccggctgc ccgcgaagca ctgggtacgt acggacgcaa
     2101 cctcggactg gcgttccaga tcacggacga catcctcgac cgaacgtcga ccgcggacgc
     2161 gctgggcaag acgccgggca aggaccaacg ggcgggcaag gcgacctacc cgcggctcta
     2221 cggcctcgag gaatcccgcc gccgcgccgc cgagctgatc gaacgcgcgc tcgatgcgct
     2281 acgctcccgg ggattgctca gcgagccgct gtcggcgctc gcgcgatacg ccgtcacgcg
     2341 cgaccgatga ccccccgccc cccacgggca gaacgcgagc ggctcgacgt gatgctcgtc
     2401 gatcgcgggc tggcgtcgac ccgcgctcgc gcgcgggcgt tgatcctcgc cggtcgggtc
     2461 cgctgcggca ccgaccggct gaccaagccc ggccagcgtt atcccatcga cgtcgagctg
     2521 accgtggacc gtggacggcg ctgggtcggc cgtggcgcct acaagctcga cgccgggctg
     2581 cgggagttcg ccgtcgacct gcgggaccgc gactgcctcg acgtcggcgc ttccaccggt
     2641 ggcttcaccg aggtgatgct cctgcgcgga gcgcaccgcg tcatcgcgct cgacgtcggt
     2701 cgcgggcaac ttgactggcg tctccgccgc gacgatcgtg tcagcgtgat cgaaggggtg
     2761 aacgcgcgcc gtctccgtgc ggacgatctt ccgttcgctc ccaccttcgc cgcgatcgac
     2821 gtctccttca tctcgatcgg tttggtgctg ccggcgattg tcgcagcgct cgaccgtgct
     2881 cgcgcgggcg agatcgtcgc gctggtcaag ccgcagttcg aggtcggccg caagcgagtc
     2941 gggcggggtg gcatcgtgcg cgacccggtg ctgcacgacg aggtcctgcg ccgcacgatc
     3001 gacctcgccg aacgcgcggg ttgcggcgcg acggggctgg ttgcctcgcc gatcgcgggg
     3061 gccgagggga accgggagtt cctgctgcac ctcgagcgcg gccgcccctc gctcgatacg
     3121 gccgccctcg accgcgcggt cgaggctgcg ctagtctttc cgctccgggc cgaggatgaa
     3181 ggatgaaccg agtcgccccg tcgcacacga tccgtcgagt agtcatcgtg gccaagctcg
     3241 ggccgtcgcc ggagggggcc cggctggccg agcgcatcgg cgagtggctc gaaggacgcg
     3301 ggatcgagcc aggcttcgag cgcaacaccg cacgcgcgat cgggcgcgag cgcggggtcg
     3361 ccggcgacga gctgtcgtcc gaggtcgatc tggtgatcgt cgccggcgga gacgggacgc
     3421 tgctgtcggt cgcgcgcgtc gcggctcccg tcggcatccc gatcctcccc gtcaatctgg
     3481 gtagcctcgg ctttctcacc gaattgcagc cgggcgagat cttcgacggt ctcgaggccg
     3541 tgctcgccgg ccactacgcg gtcgaggagc gtcagatgct gcgcgtgcgg cccgtgctgg
     3601 gtggcaaggc gcagccggag catgcgctgc tcaacgacgt cgtcatcgcc aagagcgcgc
     3661 tggcccgaat gatctcgatc gaagtccggg tcgacgacat gccggtggcg acctatacca
     3721 gcgacggtct gatcatcgcc accccgaccg gctcgacggc ttacaacctg tcggccggcg
     3781 gaccgatcct cgatccgcgg atgagcgcgt tcgtgatctc tccgatctgc ccgcacagca
     3841 tgagctaccg gccgctcgtc gtgcccggag gcgtgaccgt cgaggccatc ctgcgcagcg
     3901 acggcgaggc ggtctatctc acgctcgacg ggcaaatcgg cttcccgatg gaggtcgacg
     3961 acgcgctgac ggtcgatgcc cacccgaacc ccgtgcgcct cgtccgcatc ggggatcgcg
     4021 gcttcttcga ggtgttgcgg cgcaaactgc attggggcga gcgctagtca caatggaacg
     4081 accggctc
//
LOCUS       DASPGN010000533         4072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1153684, whole genome shotgun sequence.
ACCESSION   DASPGN010000533 DASPGN010000000
VERSION     DASPGN010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153684"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /locus_tag="VD788_15505"
     CDS             <1..1061
                     /locus_tag="VD788_15505"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HXV77720.1"
                     /translation="VPALMTSVSVHAIQRAAGQPSSLFELAARQVFYYSLFASACPLL
                     YRLGVRQRFSGGRWPRAAAAHAGTAAAVVTAVSLLGAAFEVGAGRFDGTLLQAVEREF
                     TTAQGQLNGLVNLFNYVLASGAMTIIRLTRERREHERRYAELDVRAARLEAQVARARL
                     RALEMQINPHFLFNALNGIASLIQQRRGDDAFRAIALLGELLRETIGGEGRKLVPLER
                     EAAFLERYLELERMRFGERMRSSIEVAVECRAVAVPVMILQPLVENAVRHAVAERRGP
                     GAIRVTATARDGRVVLEVRDDGPGLRAGWSLERDAGIGLQNVRHRLDALFGDRASLTL
                     ESLEPHGVVSRIVLPGDR"
     gene            1058..1813
                     /locus_tag="VD788_15510"
     CDS             1058..1813
                     /locus_tag="VD788_15510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXV77721.1"
                     /translation="MSIRTVIADDEPLARGLIRSLLDDDDEIEVVAECADGDEAVAAL
                     RALAPDLVYLDVRMPGMSGLDAMRLAGDRLPRVILVTAYERHAIEAFELRAVDYLLKP
                     LDKTRFADALARVKREMRRDSMADLGERLSPAGVAPGSRFRRCVRVRDGSIRRVVALQ
                     DVRWIEAANQYVRLHTATGDHLLSRSMSGLLRELDPRVFHRIHRSAIVNAAHVVELRA
                     ERNGAHVVVLSGGERLRLSRRRSGVLAALIGPR"
     gene            1912..3459
                     /locus_tag="VD788_15515"
     CDS             1912..3459
                     /locus_tag="VD788_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV77722.1"
                     /translation="MSTRTLRGTACVLLLLTAFAAARAQQSCQPRMEQDPSRNNLVHP
                     AGYEPAEPVSLGAVIRGGHGPTPVVLVPGAGFGGEVFAGFIEANADRYSMLAVTLPGF
                     GGTAAPPMPADGTSYAEQTWTRSARDALAHLIEQERLDRPIVVGHWISGTQVALGVAL
                     DRPDLVRGVVVVSGVTKFVSTGASMPEPRTPELRAALVDQSLAPQWFKTVTRATWDDN
                     NFLPRDYAIHPLRGLQLWRLAFEPDLQVWIRYLCEVWAADSTRDLDRLRVPLLVLQPE
                     LDELYGQGPGAGAYLQAFLGRAWEDVDDRDGLIQIRSVPASRVFVMDDQPELMNEAIR
                     RFADGPASRSRPAARPAPTEPVPVGRPMWNGRLERRGERFVVADGALSIERPDSSWEV
                     EAVDGEPPLVARISGRGSGKVSIQIQPVFGMALDAVVARIESAMATRFAEFRSLGSRP
                     TTVDGRSAIRLECHYEKEGRPERATLEFTKLDDDWLVSVAYVASPSEFDGLAAQFERI
                     RGSLDLE"
     gene            complement(3474..>4072)
                     /locus_tag="VD788_15520"
     CDS             complement(3474..>4072)
                     /locus_tag="VD788_15520"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HXV77723.1"
                     /translation="PITPVAAADQRRALDVLDEYLFDEAAFALPPETLALLKADLHED
                     WNYPWRYASDYDLGRRIAGLYDAALATLFEPARLTRVLDNERRVAAGGEVFTLPELFD
                     RLRATAFGELGRQSAERRALQRLLVDRLSGLVLAPAAGTPVEASQLAASSLRAIRREV
                     EAMLADARLDGYALAHFEDVALRAKRVLDASIRLPLPS"
BASE COUNT          558 a         1399 c         1501 g          614 t
ORIGIN      
        1 gcgtcccggc gctgatgacg tcggtcagcg tccatgcgat ccagcgcgcc gccggccagc
       61 cgagctcgct gttcgagctg gccgcgcgcc aggtgttcta ctactcgttg ttcgccagcg
      121 cctgcccgct cctctaccga ctcggcgtcc ggcagcggtt ttcgggcgga cggtggccgc
      181 gggccgctgc ggcgcacgcc ggcaccgcgg cggcggtggt gacggcggtc tcgctgctcg
      241 gtgccgcgtt cgaggtcggc gccggtcgtt tcgacggcac gctgctgcag gcggtcgagc
      301 gcgagttcac cacggcgcag ggccagctga acggcctggt caacctgttc aactacgtct
      361 tggcctcggg cgcaatgacg atcatccggc tgacccgcga acggcgcgag cacgagcggc
      421 gctacgccga gctcgacgtg cgggcggcgc ggctcgaggc acaggtcgcc cgcgcgcggc
      481 tccgagcgct cgagatgcag atcaacccgc actttctctt caatgcgttg aacggcatcg
      541 cctcgctgat ccagcagcga cggggcgacg atgccttccg cgcgattgcc ctgttgggcg
      601 agctgctgcg cgagacgatc ggcggcgagg ggcgcaagct cgtcccgctg gagcgcgagg
      661 cggcgttcct cgaacgctac ctcgagctcg agcggatgcg cttcggcgag cggatgcgca
      721 gctcgatcga ggtcgccgtc gagtgtcgcg cggtcgcggt gccggtgatg atcctgcaac
      781 cgctggtcga gaacgcggtg cggcacgccg ttgccgagcg gcgcggtccg ggcgcgatcc
      841 gcgtcaccgc gacggcccgc gacgggcggg tcgtgctgga ggtgcgggac gacggacccg
      901 gtcttcgcgc cgggtggagc ctcgagcgcg atgccggcat cggtctgcag aacgtccggc
      961 accggctcga tgccctgttc ggtgaccggg cgagcctgac gctcgagtcg ctagaacctc
     1021 acggcgtggt ctcccgtatc gtcctgccgg gggaccgatg agcatacgca ccgtgatcgc
     1081 cgacgacgag ccgctcgcgc gcgggctgat ccgcagcctg ctcgacgacg acgacgagat
     1141 cgaggtcgtg gcggagtgcg ccgacggaga cgaggccgtc gcggcgctcc gcgctctcgc
     1201 cccggacctg gtctacctcg acgtgaggat gccggggatg tcggggctcg acgcgatgcg
     1261 gctcgccggc gaccgactgc cccgtgtgat cctcgtcacg gcctacgaac gccacgcgat
     1321 cgaggccttc gagctgcggg ccgtcgacta cctgctgaaa cctctcgaca agacgcgctt
     1381 cgccgacgcg ctggcgcggg tcaagcgcga gatgagacgc gactcgatgg ccgatctcgg
     1441 cgagcggctg tcgccggccg gtgtcgcgcc gggcagccgg ttccgccggt gcgtccgcgt
     1501 gcgcgacggc tcgatccgcc gcgtggtggc ccttcaggac gtgcgctgga tcgaagcggc
     1561 caaccagtac gtgcgactgc acaccgcgac cggcgaccac ctgctgtcgc gctcgatgag
     1621 cggcctgctc cgggagctgg acccgcgggt gttccaccgc atccaccgct cggcgatcgt
     1681 caacgcggcg cacgtcgtcg agctgcgggc cgagcgcaac ggggcccacg tcgtcgtact
     1741 ctccggtggc gagcggctgc ggctgagccg gcggcgcagc ggcgtgctgg ccgcgttgat
     1801 cggcccgcgc tgagcctccc gctcgccgcc cgcaagtttt tccgaccgct cgcaagaaaa
     1861 gcccgatcgt cgctcgcttc gacccgtact gccggtcggg aggtacgagc gatgtcgact
     1921 cgaactttgc gtgggacggc ttgcgtgctg ctgttgttga cggcgttcgc cgcggctcgg
     1981 gcgcagcaga gctgtcaacc tcggatggaa caggatccga gccggaacaa cctggtccac
     2041 cccgcggggt acgagcctgc cgagcccgtt tcgttggggg cggtgattcg cggaggccac
     2101 gggccgacgc cggtcgtgct ggtgcccggc gcggggttcg gcggcgaggt gttcgccggc
     2161 ttcatcgagg ccaacgccga tcgctactcg atgctcgccg tcaccttgcc cggtttcggc
     2221 gggaccgccg cgccgccgat gcccgccgac ggaacgagct acgccgaaca gacctggacg
     2281 cggtccgcgc gcgacgcgct cgcacacctg atcgaacagg agcggctcga ccgaccgatc
     2341 gtcgtcggtc actggatcag cggaacgcag gtcgcgctgg gcgtggcgct cgatcggccg
     2401 gacctggtgc gcggcgtggt cgtcgtcagc ggcgtcacca agttcgtctc gaccggcgcc
     2461 agcatgccgg agccgcgaac accggagctg cgcgccgcgc tcgtcgacca gtcgctcgca
     2521 ccgcagtggt tcaagaccgt cacccgcgcc acgtgggatg acaacaactt cctcccgcgc
     2581 gattacgcga tccacccgct gcgcggcctg cagctctggc gcctcgcgtt cgagcccgac
     2641 ctccaggttt ggatccgtta cctgtgcgag gtctgggccg ccgacagcac gcgggacctc
     2701 gaccggctgc gggttccgct gctggtgctg caaccggagc tcgacgagct ctacgggcaa
     2761 ggaccgggcg cgggcgccta tctgcaggcg ttcctcgggc gggcctggga ggacgtcgac
     2821 gaccgcgacg ggttgatcca gatccgcagc gtccccgcct cgcgcgtgtt cgtcatggac
     2881 gatcagccgg agctgatgaa cgaggcgatc cggcggttcg ccgacgggcc ggcgagccga
     2941 tcgcgtccgg cggcgcggcc ggcaccgacg gagcccgtcc cggtcgggcg tccgatgtgg
     3001 aacggtcggc tcgagcggcg cggagagcgt ttcgttgtcg ccgacggggc gctgtcgatc
     3061 gagcgtcccg attcgtcgtg ggaagtcgaa gcggtcgacg gggagccgcc gctggtggcg
     3121 cgaatctccg ggcgtggctc gggcaaggtc tcgattcaga ttcagcccgt cttcggcatg
     3181 gcgctcgacg cggtcgtggc gaggatcgag agcgcgatgg cgacacgctt cgcggaattc
     3241 cgttcactcg gctcccggcc gacgacggtc gacggacggt cggcgatccg gctggagtgt
     3301 cactacgaga aagagggccg gccggagcga gcgacgctcg agttcaccaa gctcgacgac
     3361 gactggctgg tcagcgtggc ctacgtcgcc tcgccgtccg agttcgacgg gctggccgcg
     3421 cagttcgagc gcatccgggg cagcctcgat ctcgaatgac ccgcacgacg gggtcagctc
     3481 ggcagcggca gccggatact cgcgtcgagc acccgtttgg cgcgcagcgc gacgtcctcg
     3541 aagtgcgcga gcgcgtaacc gtcgaggcga gcgtcggcca gcatcgcttc gacctcgcgg
     3601 cggatcgccc gcagcgagga ggcggccagc tgcgaggctt caaccggcgt gccggcggcg
     3661 ggcgcgagga ccagaccgct cagccggtcg accagtaagc gttgcagggc gcgccgctcg
     3721 gcggactgcc ggccgagctc gccgaaggcg gtcgcgcgca gacggtcgaa cagctcgggc
     3781 agggtgaaga cctcgccgcc ggcggcgacg cggcgctcgt tgtcgaggac acgcgtcaat
     3841 cgtgccggct cgaacagcgt cgccagcgcg gcgtcgtaca gaccggcgat acgccggccg
     3901 aggtcgtagt cgctggcgta gcgccacggg tagttccagt cctcgtgcag gtcggccttg
     3961 agcaacgcca gcgtttccgg cggtagtgcg aaggcggcct cgtcgaacag gtactcgtcg
     4021 aggacgtcga gtgcccgccg ttgatcggcc gcggcgaccg gcgtgatcgg cg
//
LOCUS       DASPGN010000534         7620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1623289, whole genome shotgun sequence.
ACCESSION   DASPGN010000534 DASPGN010000000
VERSION     DASPGN010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623289"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VD788_15525"
     CDS             complement(<1..311)
                     /locus_tag="VD788_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77724.1"
                     /translation="MIRHVVASALVLSAVSLPRVTAAGDSDAEYKNAARALGNGEFGV
                     TLRYRYEQVAEDGFSDDAYASTLRTTLGYRSAGFYRLQAQLQFEDVSNLGLADDHNDT
                     A"
     gene            complement(416..2752)
                     /locus_tag="VD788_15530"
     CDS             complement(416..2752)
                     /locus_tag="VD788_15530"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV77725.1"
                     /translation="MNDTQTAERLVARFIERHVAGGERIPLAELCRDHPGLLEQVRQL
                     VERYTAIDDALSGELTADDGPVPVATDDALPQLDGLQTIERLGRGGMGEVYKVRDLKL
                     GRTVAAKVLRADSPLAERYGDFLREAKSLALFRDPHVVQIHELRTDGASPVILMEHVD
                     GFELTRVAPSLEFHQRASIVAEVCDAVQRAHELGIQHRDLKPANILLDERLSPKIVDF
                     GLSDGNPTRGHLVGTPAYLAPEQLDPGRPIDARSDVYALGVILYELLCGERPFAGTTQ
                     QETLDAIRAGTPRLPVEVDPRVPEPLQAIALRAMEREPADRYPAASEMARDLRRFLDG
                     RPVLARPTLYRTALGRRLLPHLEQIDEWLDLRLIYPHEARRLKRGYALLEAREDDWIV
                     QSRMLSPSQIALYLGGFVLMCGALLYFGAHRFHQAVVGVAGPALVLGLPFAALNLSAA
                     WLYRREHRAAAIAFYLAATALLPLFLLIVFHETGAWPGPSAGETQFFEDGSVSNRQLQ
                     IAAATTLAWTAWLAARTRTVGLSSVATLLAGLFALSVLTDFGLRRWIERGEWDRLALA
                     LLPLLALQAAVAVRLDRIRWAWFSRPLFVGCSLLLVAVCELFALDGRALAFLRLSLAR
                     FQAADVSSPALLDTVAAMTAAGLLFYLLGAVLERRGTQAMAPAAWLLIAISPFAMLEP
                     LAYLVNTGEYAPRLDWLYLIVAVIIAWLSHYHQRKSFYLAGLVNTGVALSFITYNRGW
                     ADRPAWAVAVVLAGLVALGAGLGLAVRERRRRATPRDG"
     gene            complement(2749..3327)
                     /locus_tag="VD788_15535"
     CDS             complement(2749..3327)
                     /locus_tag="VD788_15535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV77726.1"
                     /translation="MDDEQTTGLLRQARAGSTDALDALYRRYAGRLLALIRLRMGPEM
                     RSRLESRDVLQSCLLKSFERIDQFEHAHDGSLMAWFARIAENEIRDRIKFERRRRRDV
                     RQELPLDAAPRALEQRVRSSLSRLILGERAAELERALDELPADYREVILLRKLEERSW
                     DEIGARFGKSPDACRMLLARAMTALTMRMEHG"
     gene            3463..4068
                     /locus_tag="VD788_15540"
     CDS             3463..4068
                     /locus_tag="VD788_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77727.1"
                     /translation="MRETGDPRRTACLAALLLVAAPIGANPADDDGAPARIRIEGDLS
                     VTTDDDRRTLTVASLEHDFVVVLPYLDEWMLETDDRSVFTGRSPRYTFALSRRDEIED
                     EAREVLEQALDELLIGGADIRFLQFVERDRRDVLAFQVRHSEQGPWLWFYWMLFPTTS
                     GWLQLNVVDTRPAELAPLDIDLVETLSRATTVSEVLGPVSD"
     gene            complement(4109..4513)
                     /locus_tag="VD788_15545"
     CDS             complement(4109..4513)
                     /locus_tag="VD788_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77728.1"
                     /translation="MKGTAHLVGGCRCDKAEQRIEAPPSGKGVRCPSERARRASERWD
                     SIVIPSVVPGAIVEWKYNRYFDNRLPLWIEGFEGDVPTLLRRIEFDKPHRFEYRIAFS
                     DRHGRQHRPRRDGNTTRIGRFATDGPIVTTAE"
     gene            4558..5349
                     /locus_tag="VD788_15550"
     CDS             4558..5349
                     /locus_tag="VD788_15550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV77729.1"
                     /translation="MRFAKLRLATGPSLHYAEHGDPDGEPVLFLHGWPDSWFSFSRVL
                     PLLPERLRAVVIDQRGFGDSERPQTGYSIPELAADAIAVLDALEIERATLVGHSYGSF
                     VARRAAIARPERVAALLLIGTGFVSSNPVIRDLQNALRDLPDPVPVEFARDFQASTVH
                     HPVPAEFFQRIVAESLKLPARLWRLAIDRLAAYDDVRELARIDAPTRLLWGDHDALFP
                     RSDQDAFLAALPAARLTVHEATGHSPDWERPELVAAAIETHAPQR"
     gene            5352..6770
                     /locus_tag="VD788_15555"
     CDS             5352..6770
                     /locus_tag="VD788_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77730.1"
                     /translation="MDRDLRRCLLLPGIVLLAGLGLVRAAPAAGVRAASYEIEVRCFP
                     DESRLEGQARIRLAPAGRVAGTVRFWLHGELRATAVRAGRAELEFSQQRDYYEWDYSL
                     IGNRVEVSLPKGAVPELLEVTWGGPLNPSKARSPSDYMRIDADGVFLRALGYSPWFPL
                     FLDEDGDDHAVDFDLVRIEAPQELTAVFAGDHLRSTTDGKLTRSEWRARGLSLFDAQL
                     TVRRFTILREGAIRVYPLRDEASVESAESILGFTRALLAYYTEHYAEDAVVGQLHVVE
                     MPEFGDISSRNVVGIQQSSWRSFERSDGSKRTLAHELVHPFVQPDVARSDPLYALVIE
                     GFPSYFHYPALAAIGALDYDRRISEVRQSYLTKRRTGRDLHGRPLPTEKPITRITADE
                     IGLYKDVFVLADRALLFLDDLRREMGEERFHAFTRELFALESVDDRAFRALVLGHLPD
                     QRARLTLWLDTTELSEPESVGH"
     gene            6878..>7620
                     /locus_tag="VD788_15560"
     CDS             6878..>7620
                     /locus_tag="VD788_15560"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77731.1"
                     /translation="MRQLRTAGISTLLVPALLLLLASCADAPVDDGKIPVTTRSDEAR
                     ALFLEARDLNDRLRGVEALPLLERAVGHDPTFAQGHWLLAQVQPTAAGFFANLEQARV
                     HAASASQGERWLIEATDAAVNGDPAGQGEILQQLVAAFPEDERAHTLLGTYFFGQQRW
                     AEALSEYERAIAIAPEFSPPYNQLGYAYRFTERFAEAESAFRKYIELIPDDPNPYDSY
                     AELLLKLGRYEDSIAQYEKALEQQSTFAAS"
BASE COUNT         1192 a         2682 c         2572 g         1174 t
ORIGIN      
        1 ttcgccgtgt cgttgtggtc gtcggccagc cccaggttcg aaacgtcctc gaactgcagc
       61 tgggcctgca gccggtagaa gccggccgag cggtacccga gcgtggtacg cagcgtcgag
      121 gcgtaggcgt cgtcggagaa gccgtcctcc gcgacctgct cgtagcggta gcgcagcgtc
      181 acgccgaact ccccgttgcc caaggcccgg gcggcgttct tgtactccgc gtcgctgtcg
      241 ccggccgcgg taacccgagg cagcgacacc gccgagagga cgagcgccga tgcgacgacg
      301 tggcgaatca cgcttcggcc tgtcgattca gcgcgagccg ccggcacggt gtggtcatcc
      361 ggatgcctcc ctcagcgacg cccgtcagcg tagcacgcgc ggctcggtcg acggctcagc
      421 cgtcgcgggg tgtcgcgcgg cgccggcgct cgcgcacggc caatccgagc ccggcgccga
      481 gcgcgacgag cccggcgagc acgacggcga cggcccatgc cggccggtcg gcccagccgc
      541 gattgtaggt gatgaacgac agcgccacgc cggtgttgac cagcccggcg aggtagaagc
      601 tcttgcgctg gtggtagtga ctcaaccagg cgatgatcac cgcgacgatc aggtagagcc
      661 agtcgaggcg cggcgcgtac tcgcccgtgt tgaccagata ggccagcggt tcgagcatcg
      721 cgaacggcga gatggcgatc aacagccacg ccgcgggcgc catcgcctgc gtcccgcgcc
      781 gctcgagcac ggcgcccagc agatagaaca gcagcccggc cgcggtcatc gctgccaccg
      841 tgtcgagcag cgccgggctc gatacgtcgg ccgcctggaa ccgggcgagc gacaggcgca
      901 ggaaggcgag cgcgcgtccg tcgagcgcga acagctcgca gaccgcgacc agcagcagcg
      961 agcatccgac gaacagcggc cgggagaacc aggcccagcg aatgcgatcc agccggaccg
     1021 cgaccgccgc ctgtagcgcg agcagcggca gcagtgccaa cgcgagacga tcccactctc
     1081 cccgctcgat ccagcgccgc aggccgaagt cggtcagcac ggagagcgcg aacaaaccgg
     1141 cgagcagcgt cgcgacgctg ctcagcccga cggtgcgcgt gcgcgcggcg agccacgccg
     1201 tccaggccag cgtcgtggcg gccgcgatct gcagctgccg gttcgacacg ctgccgtcct
     1261 cgaagaactg cgtctcgccc gcgctcgggc caggccacgc gccggtttcg tgaaagacga
     1321 tcaacagaaa caacggcagc agcgcggtgg cggccaggta gaacgcgatc gccgccgcgc
     1381 ggtgctcgcg gcgatagagc caagcggccg acaggttgag cgcggcgaac ggcaggccca
     1441 gcaccagcgc cggcccggcg acgccgacga ccgcctggtg gaaccgatgg gcgccgaagt
     1501 acagcagcgc gccgcacatc agcacgaacc cgccgagata cagcgcgatc tgcgacggcg
     1561 acagcatccg actctgtacg atccaatcgt cctcgcgcgc ctcgaggagc gcgtagccac
     1621 gcttcagtcg ccgcgcctcg tgcgggtaga tcagacgcag gtcgagccat tcgtcgatct
     1681 gctcgaggtg cggcaggagc cgccgaccga gcgccgtgcg atagagcgtc ggccgggcga
     1741 gcaccggacg gccgtcgaga aaacgccgca ggtcgcgcgc catctcgctc gccgccgggt
     1801 agcggtcggc cggctcgcgc tccatcgcgc gcagcgcgat cgcctggagc ggctccggca
     1861 cgcgcgggtc gacttcgacc ggcaggcgcg gcgtgcccgc gcggatcgcg tcgagcgtct
     1921 cctgctgggt cgtgccggcg aacggtcgct cgccgcacag cagctcgtag aggatgacgc
     1981 ccagcgcata gacgtcgctc cgcgcgtcga tcggccggcc cggatcgagc tgctccggcg
     2041 cgaggtaggc cggtgtcccg accaggtggc cgcgtgtcgg gttgccgtcg ctcaggccga
     2101 aatcgacgat cttcggcgac agccgttcgt cgagcaggat gttggccggt ttcaggtcac
     2161 gatgttggat accgagctcg tgggcgcgct gcaccgcgtc gcagacctcg gcgacgatgc
     2221 tcgcgcgctg gtggaactcg agcgacggag cgacccgcgt cagctcgaag ccgtcgacgt
     2281 gctccatcag gatcacggga gacgcaccgt cggtgcgcag ctcgtggatc tgcaccacgt
     2341 gcgggtcgcg gaacagcgcg agcgacttgg cttcccgcag gaaatcaccg taacgctcgg
     2401 ccaacgggct gtccgcacgc agcaccttgg ccgccaccgt ccggcccagc ttcaggtcgc
     2461 ggaccttgta gacctcgccc atcccgccgc ggcccaggcg ctcgatcgtc tgcaggccgt
     2521 cgagctgtgg gagggcgtcg tcggtcgcga ccggcacggg cccgtcgtcg gccgtcagct
     2581 cgccgctcag cgcatcgtcg atcgccgtgt aacgctcgac caactggcgg acctgctcga
     2641 gcagccccgg gtggtcgcgg cacagctcgg cgagcgggat gcgctcgccg ccggcgacgt
     2701 gtcgctcgat gaaccgtgcg acgagacgtt ctgcggtctg cgtgtcgttc atccgtgctc
     2761 catccgcatc gtcagcgcgg tcatcgcgcg ggccagcagc atccggcagg cgtcggggct
     2821 cttgccgaac cgcgcgccga tctcgtccca gctccgctcc tcgagcttgc gcagcagaat
     2881 gacctcgcgg tagtcggcgg gcagctcgtc gagcgctcgc tcgagctcgg cggcgcgctc
     2941 tccgaggatc agccggctca acgacgatcg tacccgttgc tcgagcgcac gcggcgcggc
     3001 gtcgagcggc agctcctgtc ggacgtcgcg gcgccggcgc cgctcgaact tgatccggtc
     3061 cctgatctcg ttctcggcga tgcgcgcgaa ccacgccatc agcgacccgt cgtgggcgtg
     3121 ctcgaactgg tcgattcgct cgaacgactt gagcaggcaa ctctgcagca cgtcgcgcga
     3181 ctcgagccgc gagcgcatct cgggacccat gcgcaggcgg atcagcgcca gcaggcggcc
     3241 ggcgtagcgc cggtacaacg cgtcgagcgc gtcggtcgac ccggcgcgcg cctgccgcaa
     3301 cagaccggtg gtctgctcgt catccacgga atcaccctag caaatccgcg ggataagacg
     3361 acggtgacgg cctcgaccga acgccgcgcg ggcagtgcgg tgtcggccga tcgggagtag
     3421 actcgttgcg gcggaaatcg cccggattca gcggagccgc ggatgagaga aaccggagac
     3481 ccgcgtcgca cggcctgcct ggcggcgctg ctgctggtcg ctgcgccgat cggtgcgaac
     3541 cccgccgacg acgacggggc cccggcccgg atccggatcg agggagatct ctccgtcacc
     3601 accgacgacg accggcgcac gttgaccgtg gccagcctgg agcacgactt cgtcgtagtg
     3661 ctgccgtact tggacgagtg gatgctcgag accgacgacc ggtcggtttt caccgggcgt
     3721 tcgccgcgct acaccttcgc cctgagtcgc agagacgaga tcgaggacga ggcacgcgag
     3781 gtactggagc aggcgctcga cgagctgctc atcggcggcg cggacatccg cttcctgcag
     3841 ttcgtcgaac gcgaccggcg tgacgtcctc gccttccaag tccgtcactc cgagcagggg
     3901 ccgtggctct ggttctactg gatgctgttt cccacgacca gcggctggct gcaattgaac
     3961 gtggtcgaca cgcggccggc ggagctcgcg ccgctcgaca tcgatctcgt cgagacgttg
     4021 agccgagcca ccaccgtttc cgaggtgctc gggccggtat ccgactgatc gatcgaccac
     4081 gagccgatcg acgtcgcgac cggtgcgctc actccgcggt tgtgacgatc ggcccgtcgg
     4141 tcgcgaacct gccaatccgc gtggtgttac cgtctcgtcg tggcctatgc tggcggccat
     4201 ggcgatcgct gaaagcgatc cgatactcga atcgatgcgg tttgtcgaat tcgatgcgac
     4261 gcaacaaggt aggtacgtca ccctcgaacc cctcgatcca cagcggcagc cgattgtcga
     4321 agtatcgatt gtacttccac tcgacgattg cgcctggcac gacgctcggg atgacgatcg
     4381 agtcccagcg ctcgctcgcg cgcctcgcgc gctcactcgg gcatcgaact cctttgccgc
     4441 tgggaggggc ctcgatgcgt tgctccgcct tgtcgcatcg acagcccccg accaggtgcg
     4501 ccgttccttt catgctgcca ccgtgctagt gtgaatcaac acccggaggg tccgacggtg
     4561 cgattcgcga agctgcggct ggcgaccggt ccttctctcc attacgcgga acacggcgat
     4621 cccgacggcg agcccgtcct gttcctccac ggctggcccg actcgtggtt ctcgttcagc
     4681 cgggtgttgc cgctgctgcc cgaacgccta cgcgccgtcg tcatcgacca gcgcggcttc
     4741 ggcgactcgg agcgcccgca aaccggctat tcgattcccg agctcgcggc cgacgcgatc
     4801 gccgtcctcg acgccctcga gatcgagcgc gcgacgctcg tcggtcattc ctacggcagc
     4861 ttcgtcgcgc ggcgggcggc gatcgcgcgt ccggagcggg tcgccgcgct gttgttgatc
     4921 ggcaccggct tcgtgtcatc caatccggtc atacgggatc tgcagaacgc cctgcgtgac
     4981 ctgccggatc cggtacccgt cgagttcgcc cgtgatttcc aggcgagcac cgtgcaccac
     5041 ccggtcccgg cggagttctt ccagcgaatc gtcgccgaga gcctgaagct gccggcgcga
     5101 ctctggcgtc tcgccatcga tcgactcgcg gcgtacgacg acgtgcgaga gctcgcccgc
     5161 atcgacgccc cgacgcggct gttgtggggc gatcacgacg cgctgttccc gcgatcggac
     5221 caagacgcct tcctcgcggc gttacccgct gcccggttga cggtccacga ggccaccggc
     5281 cactctccgg actgggagcg tcccgagctg gtggcggccg cgatcgagac gcacgcgccg
     5341 caacgctgaa agtggaccgt gacctgcgcc gctgtctgct gttgccgggc atcgttctcc
     5401 tggccggcct cggtctcgtc cgagccgctc cggccgcagg cgtacgagcg gcgtcgtacg
     5461 agatcgaggt gcgctgcttc cccgacgagt cgagactcga ggggcaggca cggatccgtc
     5521 tcgcaccggc cggccgggtc gccgggacgg tgcgcttctg gctccacggg gagctgcggg
     5581 cgacggccgt gcgtgccggc cgggcggagc tcgagttttc gcagcagcgc gactactacg
     5641 agtgggacta ttcgctgatc ggcaaccgcg tcgaggtctc cctgcccaaa ggagcggtac
     5701 ccgagctgct cgaggtgacc tggggcggcc cgctgaaccc gtcgaaagcg cgcagcccct
     5761 ccgactacat gcgaatcgac gcggacgggg tcttccttcg tgccctgggg tactccccgt
     5821 ggtttccgct cttcctcgac gaagacgggg acgaccacgc cgtcgacttc gatctcgtgc
     5881 gcatcgaagc gccgcaggag ctgaccgcgg tgttcgcggg cgatcacctg cgctcgacca
     5941 ccgacgggaa gctgacacgg tcggagtggc gcgcgcgcgg gctttcgctg ttcgacgccc
     6001 aactcaccgt tcgccggttc actatcctcc gagaaggagc gatccgcgtc tatccgctgc
     6061 gcgacgaggc ctcggtcgag tcggccgaga gcatcctcgg cttcacccgc gcgctgctcg
     6121 cctactacac cgagcactac gccgaggacg ccgtggtcgg ccagctccac gtcgtcgaga
     6181 tgccggagtt cggcgacata tcgagccgga acgtcgtcgg catccagcag tcctcctggc
     6241 gatcgttcga gcgctcggac ggatcgaaac gaaccctggc gcacgaactg gtccatccgt
     6301 tcgtccagcc ggatgtcgcg aggtcggacc cgctgtacgc gctcgtgatc gaggggtttc
     6361 cctcctattt tcactatccg gccctcgccg cgatcggtgc actcgattac gaccgccgga
     6421 tttccgaagt gcgccagagc tacctgacga agcggcgcac cggacgggat ctgcacggcc
     6481 ggccgcttcc gaccgagaag ccgatcaccc ggatcacggc cgacgagatc gggctgtaca
     6541 aggacgtgtt cgtgttggcc gaccgggccc tgctgtttct cgacgatctg cggcgagaga
     6601 tgggcgaaga gaggttccac gccttcaccc gcgagctgtt cgcgctggag tcggtggacg
     6661 atcgggcctt ccgcgcgctc gtgctcggcc atctcccgga tcagcgggcg cgtttgacgc
     6721 tctggctcga tacgaccgag ctgagcgaac cggagtcggt ggggcactga gcacgcgtgg
     6781 tcggccgaaa gtgcggcgtg ccgcaacttt cgctcccgga tcccgtacgt ggagaccagc
     6841 cccgcctcgc gtcttcgggg cagacaagga gagcttcatg aggcaactcc gaaccgctgg
     6901 aatttcgacc ctgctcgtcc cggcgctgct gctgctcctc gcgtcctgcg cggacgcgcc
     6961 ggtggacgac ggcaagatcc ccgtcacgac ccgctccgac gaggcgcgag cgctgttcct
     7021 cgaggcgcgc gacctcaacg accgtttgcg cggcgtcgag gccctgccgc tgctcgaacg
     7081 agcggtcggg cacgatccga ccttcgctca gggccactgg ctcttggcgc aggtccaacc
     7141 gacggcggcc gggttcttcg ccaacctcga acaggcgcga gtccacgcgg cctcggcctc
     7201 gcagggcgag cgctggctga tcgaggcgac cgacgcggcg gtcaacggcg acccggcggg
     7261 acagggggag atcctgcagc agctcgtcgc ggcattcccc gaggacgagc gagcgcacac
     7321 cttgctcggg acctacttct tcggacagca gcgctgggcg gaagcgctct ccgagtacga
     7381 gcgggcgatc gccatcgctc ccgagttttc accaccctac aaccagctgg gatacgccta
     7441 tcgcttcacc gagcgattcg ccgaggccga atccgcgttc cgcaaataca tcgagctgat
     7501 cccggacgac cccaacccgt acgactccta cgccgagctg ctgctcaagc tcggacgcta
     7561 cgaggattcg atcgcgcagt acgagaaggc gctcgagcag cagagcacct tcgccgcgtc
//
LOCUS       DASPGN010000535         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1271163, whole genome shotgun sequence.
ACCESSION   DASPGN010000535 DASPGN010000000
VERSION     DASPGN010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1271163"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VD788_15565"
     CDS             complement(<1..487)
                     /locus_tag="VD788_15565"
                     /inference="COORDINATES: protein motif:HMM:NF024977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV77732.1"
                     /translation="MASRQREISVSEFFTKNRHLLGFDNPQKALLTAVKEAVDNSLDA
                     CEEAGILPDLRIEIEELSEDRYRVAVEDNGPGIVRQQIPKIFGKLLYGSKFHRLKQQR
                     GQQGIGISAAGMYAQLTTGRPLVITSRTSRRAPAHLFHIGIDTKKNAPVIQRDVEVDW
                     QP"
     gene            748..1308
                     /locus_tag="VD788_15570"
     CDS             748..1308
                     /locus_tag="VD788_15570"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HXV77733.1"
                     /translation="MLDEDAANAEPEPRDRPTGRLARRLLVLAAIAYASGIVIVPLLE
                     QAGWTAAGLGHLLYAPVCHQLPERSFAVGEHPLSVCARCTGLYAGGVIGLLTGTWIAS
                     RRAPAPRPLWLAVLAFPSLVDATLPRLGVAEGLTSLPRMGLALPPGMLLGIYLAIGIH
                     DLFVSRTKLRATRTECPASVLEGLDG"
     gene            1301..1831
                     /locus_tag="VD788_15575"
     CDS             1301..1831
                     /locus_tag="VD788_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77734.1"
                     /translation="MDKAAPSMLRATLIGGAASGFVSGLPVVGALNCLCCSLVVGGGF
                     LAAFLYSRQCERSGVGFRPGQGAWVGLVAAVFFALASTVTGAAARAVMPAPDLDQMTE
                     MMDQLDLPPEAVDMAIQMLEMGGTALGILFMFLLNLLIGALFSTVGGLIGGAAFKVNV
                     PPPPRETGPPTLGSQP"
     gene            2150..2851
                     /locus_tag="VD788_15580"
     CDS             2150..2851
                     /locus_tag="VD788_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77735.1"
                     /translation="MITGFNTDIEHLGRVFHVQTEDKGLDNPVVETLVYCGGEIIDAR
                     KNSYADLAAAGECSEDEILVRMETQHHALIREIRNGKFDTGGPKPFGFNIISNRSLDE
                     VVLSFLHEQDRLSAISLDLVDRVTLREGQHTRVRLHVVEGATGRSIAGAQVKATLIST
                     QHMPRELASGVSDDSGRVDLDFDLPALGQAELAVLLRAGAAGRRAVRREVVQRSAGSP
                     VGGSQEPAAEGSPVD"
     gene            complement(2855..3322)
                     /gene="smpB"
                     /locus_tag="VD788_15585"
     CDS             complement(2855..3322)
                     /gene="smpB"
                     /locus_tag="VD788_15585"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXV77736.1"
                     /translation="MVAKGDKDGSERVLASNRAASHEFQLLKRFEAGVVLTGPEVKTA
                     RAGRVNLRDGYARIKHGEVLLFGVHFSPYAHARAEAQNPLRVRKLLLHAREIRKLERE
                     TQSSGTTLVPTKLYLKQGRIKIEIALARAKRLFDKRESAKKKIQQREMARGRE"
     gene            complement(3370..4215)
                     /gene="rsmI"
                     /locus_tag="VD788_15590"
     CDS             complement(3370..4215)
                     /gene="rsmI"
                     /locus_tag="VD788_15590"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HXV77737.1"
                     /translation="MAGKLFVVATPLGNLGDISQRALDTLRRVEMIACEDTRRTSRLL
                     AAYEIRTPLLSCHRFNEQARLAGILDRLGRGDDVALVSDGGTPVVSDPGGELIRAAVD
                     AGVAVVPIPGGSAVTALLSIAGLSADRFVFDGFLPHRGGERRRRLRELRDETRPVVVF
                     ESPRRIVATLEDVDRVLGRRPLVLGRELTKVHETVLRGTALELIAALDRPVRGELTLL
                     IAGTRPGDTPAAERDADALRRAWGEALAGAGGDRRKALKDAARALGLRRAELHRRLVE
                     LGESV"
BASE COUNT          645 a         1510 c         1533 g          742 t
ORIGIN      
        1 cgggttgcca gtcgacctcg acgtcgcgct gaatcaccgg tgcgttcttc ttggtgtcga
       61 tgccgatatg gaacagatgg gccggcgcgc gccgcgacgt gcgtgaagtg atcaccagcg
      121 ggcgtcccgt cgtcagctgg gcgtacatgc cggcggcgga gatgccgatg ccttgctgcc
      181 cgcgctgctg cttcagccgg tggaacttcg agccgtagag cagcttgccg aagatcttcg
      241 ggatctgctg ccgaacgatg ccaggcccgt tgtcctcgac ggcgacgcgg tagcggtcct
      301 cgctcagctc ctcgatctcg atccgcaggt cgggcaggat gcccgcctcc tcgcaggcgt
      361 cgagggagtt gtcgaccgcc tccttgacgg cggtcagcag cgccttctgc gggttgtcga
      421 acccgagcag gtgacggttc ttcgtgaaga actcggagac cgagatctcg cgctggcgcg
      481 aggccatcgt gtgggcgtcc tcgaggacgc gcttgcccct gctcgacact cgtttcgttt
      541 ggctcggtcg aggcgcggca ccggcgtcgc ctggagcgcg agcgggttcg aacaggccgg
      601 gctgacgtcg atcgatccgc gtcggtcggg agcttcccat cggacgacca cctttcacga
      661 cccgacaacg ggcggccgag cattctacca gcgacggggc gaagtcggag ggttcgccgg
      721 cgtcgcggtt tcgagtaaga tcggccgatg ctcgacgagg acgcagcgaa tgccgaaccg
      781 gagccgcggg atcgaccgac cggccggttg gctcggcggc ttctggtgct ggccgcaatc
      841 gcctacgcct ccgggatcgt gatcgtgccg ctgctcgagc aggccggttg gaccgccgcg
      901 ggtctcgggc acctgctgta cgcgccggtc tgtcatcaat tgcccgagcg cagcttcgcc
      961 gtcggcgagc acccgctgtc ggtttgcgcg cgctgcacgg gtctgtacgc cggcggcgtg
     1021 atcgggcttc tcaccgggac ctggatcgcc tcgcggcgcg cacccgcccc gcgaccgctg
     1081 tggctcgcgg tgctcgcgtt tccgtcgttg gtcgacgcga cgctgccgag gctcggcgtc
     1141 gcggaagggt tgaccagcct cccgcggatg gggttagcac tgccgccggg catgctgctc
     1201 gggatctacc tcgccatcgg gatccacgac ctgttcgtct cgcgcaccaa gctacgagcc
     1261 acgcggaccg agtgcccggc atcggtcttg gagggattgg atggataagg ctgcaccgtc
     1321 gatgttgaga gccaccctga tcggtggcgc tgcctcgggc ttcgtctccg ggttgccggt
     1381 cgtcggcgcc ctcaactgtc tgtgctgttc gctcgtggtg gggggcggct tcctcgcggc
     1441 attcctctac tcgagacaat gcgagcgtag cggtgtcggc ttccgccccg gccaaggtgc
     1501 ttgggtcggt ctggtcgccg ccgttttctt cgcactggcg tcgacggtca ccggtgcggc
     1561 ggcccgcgcg gtgatgccgg ctcccgacct cgaccagatg acggagatga tggaccagct
     1621 ggacctgcca cccgaggcgg tggacatggc gattcaaatg ctcgaaatgg gcgggacggc
     1681 gttgggcatc ttgttcatgt tcttgctgaa cctgctgatc ggggcgctct tctcgacggt
     1741 cggcggcctg atcggcggtg cggcgttcaa ggtcaacgta ccgcctccgc cgcgggagac
     1801 cggtcccccg accctcggtt cgcagccgtg acccgatgtc cgtcggcgga cacggcgtgt
     1861 ttgccgacgg acgcggcgag cggcgcgacg agaactcgcc cggccggatc tcgccgtcgt
     1921 cccggggctc ggaccgtccc ggacaggctg gcccgtccgt tgcttcggga agatccgcgg
     1981 cgggggcgtc gcccccggcc gccgtcctcg ggaacgggac cctcgagggc ggtggagagt
     2041 gccgccgggc cgggcgaccg gagcgatcgt ggaggcggct ccggtcgccc gcccgtcctc
     2101 ggcagacggc ttgacacccg ttccgtcgac tgactacgtt tacctaccca tgatcacggg
     2161 cttcaacacc gacatcgaac acctcggacg tgtgttccac gtccagaccg aggacaaggg
     2221 tctcgacaac cccgtcgtcg agacactggt ctactgcggc ggggagatca tcgacgcgcg
     2281 gaagaactcc tacgccgacc tggctgcggc aggggagtgt tccgaggacg agatcctcgt
     2341 gcgcatggag acccagcatc acgcgctgat ccgcgagatc cgcaacggca agttcgatac
     2401 cggcggcccc aagccgttcg gtttcaacat catcagcaac cgcagcctcg atgaggtcgt
     2461 tctgagcttc ctgcacgagc aggaccggct gtcggcgatc tcgctcgatc tcgtcgatcg
     2521 ggtgacccta cgcgagggcc agcacacgcg ggtccggctg cacgtcgtcg agggtgcgac
     2581 gggccgctcg atcgccgggg cgcaggtgaa ggccacgttg atctccacac agcacatgcc
     2641 cagggagctc gcctccggcg tttcggacga ttccggccgg gtcgacctcg acttcgatct
     2701 tcccgccctc gggcaagccg agctggcggt gctgctgcgg gccggtgcgg ccggtcgccg
     2761 ggcggtgcga cgggaggtcg tccagcgttc cgccggtagc ccggtcggcg ggagccaaga
     2821 gccggccgcc gagggaagtc cggtcgattg acgactactc gcggccgcgg gccatttccc
     2881 gctgctggat cttcttcttc gcggattccc gcttgtcgaa cagtcgtttc gcgcgcgcca
     2941 gcgcgatctc gatcttgatc cgcccctgct tcaggtagag cttcgtcggt accagcgtcg
     3001 tgccggacga ttgcgtctcg cgttccagct tgcggatctc gcgcgcgtga agcagcagct
     3061 tgcgaacccg caggggattc tgggcttcgg cgcgcgcgtg tgcgtacggg ctgaagtgga
     3121 caccgaacaa caggacctct ccgtgcttga tccgcgcata cccgtcacgc agattgaccc
     3181 gtccggcgcg cgccgtcttc acctcgggcc cggtcaagac gaccccggcc tcgaaacgct
     3241 tgagcagctg gaactcgtgc gaggcggcgc ggttgctggc cagcacgcgc tcggatccgt
     3301 ccttgtcgcc cttcgcaacc acgaccgctc cggtgggtca aaacgaagga gtatagcagc
     3361 gccggccgct cagaccgact ctcccagctc cacgagccga cggtggagct ccgcccgccg
     3421 caacccgaga gcccgcgccg cgtccttcaa cgccttgcgc cggtcgccac cggctcccgc
     3481 caacgcctcg ccccaggctc gtcggagcgc gtccgcgtcg cgctcggccg ccggcgtgtc
     3541 gccgggccgc gtgccggcga tcagcaaggt caactcgccg cggacgggcc ggtcgagtgc
     3601 cgcgatcaac tcgagagccg tgccgcgcag caccgtctcg tggaccttgg tcaattcgcg
     3661 gccgaggacc agcggccgtc gaccgagcac gcggtcgacg tcttcgagcg tggccacgat
     3721 tcgccgcgga gattcgaaga cgacaaccgg gcgggtctcg tcgcgcagtt cacggagacg
     3781 gcgccggcgc tcgcccccgc gatggggcag gaagccgtcg aagacgaagc gatcggccga
     3841 caatccggcg atcgacagca gcgccgtgac cgcggagccg cccggaatcg gtacgacggc
     3901 gacacccgcg tcgaccgcgg cacggatcag ctcgccgccg ggatcggaga cgaccggtgt
     3961 gcctccgtcc gagacgaggg ccacgtcgtc tcctcgtccg aggcgatcga ggatcccggc
     4021 cagtcgagcc tgctcgttga agcggtggca ggacagcagc ggggtgcgta tttcgtaggc
     4081 ggcgagcagc cgcgacgtcc ggcgcgtgtc ttcgcaggcg atcatctcga cccgtcgcag
     4141 cgtgtccagc gcgcgctgcg agatgtcccc caagttgccc agcggggtcg cgacgacgaa
     4201 caacttgccg gccacgcgtc gagcatacca tgcgaccccg attcgcccgt cgcggcggac
     4261 atgggtagaa tgcacggacg agcgagccaa tcgcaatcga gggtgatcga tgagcctgtt
     4321 gaccaagacg atcgtcgcag cgagtgtgac gctggcgctg tccctggtgg cgatcggcgc
     4381 cgcgccgcaa ggcggcgatc cggctaccga tcgagacgtc acgcgaaccc
//
LOCUS       DASPGN010000536         3067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1623697, whole genome shotgun sequence.
ACCESSION   DASPGN010000536 DASPGN010000000
VERSION     DASPGN010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623697"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..137
                     /locus_tag="VD788_15595"
     tRNA            63..137
                     /locus_tag="VD788_15595"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:96..98,aa:Glu,seq:ctc)
     gene            complement(132..491)
                     /locus_tag="VD788_15600"
     CDS             complement(132..491)
                     /locus_tag="VD788_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77738.1"
                     /translation="MELSTEHPLFVGFQIDGSLRRQIEALSGPERRYVSSDDSTFLMM
                     CRKGDDVYVGKLIEDGLTTDRVDDVRRNVLSIMRRLCPDVRLPNHLEIWPTARAGSEP
                     SGQPPDPSAPRSLPREW"
     gene            complement(494..1858)
                     /locus_tag="VD788_15605"
     CDS             complement(494..1858)
                     /locus_tag="VD788_15605"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="capsular polysaccharide biosynthesis protein;
                     catalyzes the formation of D-mannose 6-phosphate from
                     alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HXV77739.1"
                     /translation="MAGGQPAGLASFKAYDIRGRVPDELDRELAYKVGRAYARFVGPK
                     RVVVGHDIRLSSPEIAGALIDGLNAAGVDVVDIGQCGTEQVYFSTFQLKLDGGIMVTA
                     SHNPKDYNGLKLVREQARPISSDTGLLEMQRMILDDELGPEPGGGRTTSADDSAAFVD
                     HLTGYVDTGALEPLTIVVNAGNGGAGRIVDLLEPRLPFRFVKLGHEPDGTFPNGVPNP
                     LLQENRETTSRAVRESGADFGVAWDGDFDRCFLFDEKGSFIEGYYIVGLLAASILAKN
                     PGARVVHDPRLTWNTIEVVREAGGVPIQSKSGHAFVKQVMREQDAVYGGEMSAHHFFR
                     DFSYCDSGMVPWLLVAELICRAQRPLSGLVGERMQRYPASGEINRRLADPAGALDRVR
                     RHYSRDALRVDETDGIGLDFERWRFNLRLSNTEPLIRLNVESRGDPALMRAKTDEVLA
                     LLEE"
     gene            1965..>3067
                     /locus_tag="VD788_15610"
     CDS             1965..>3067
                     /locus_tag="VD788_15610"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HXV77740.1"
                     /translation="MSADETRIERDSMGEMEVPRDAYYGASTQRAVLNFPISGLRIPS
                     PMIRALGLLKWAAARVNAELGRLDPRLAEAIARAAREVADGKLDDAFVVDLFQTGSGT
                     SSNMNANEVIANRAAELLGAGRGARAVLHPNDHVNLGQSSNDIIPTAVHLGALLAINE
                     SLDPGLALLRGALERKSEELWSTIKTGRTHLQDATPIRMGQVFRGYAGQLERAERRVE
                     WAKQQLGEVALGGTAVGTGINTHPEFAARVCAIVSADCAVSIHETDNHFQAQATLDSV
                     VAASGALRTVAISLLKIANDVRLLASGPRTGLRELTIPEVQPGSSIMPGKVNPVIAES
                     LIQASAQALANDQAIVQAGEWSFFELNTMLPLAG"
BASE COUNT          484 a         1055 c         1030 g          498 t
ORIGIN      
        1 aggggttcga atcctcttcc cccagccact cttatcgtct ccgcgcagtg gaagccggat
       61 ggggcgctat cgtctagggg tcaggacggg tggttctcag ccatcaaacc ggggttcgaa
      121 tccccgtacc gctaccattc cctcggtaac gaacgcggcg cgctcgggtc gggtggctgg
      181 ccgctcggct cgctgccggc tcgcgcggtc ggccagatct cgagatggtt cggcaggcgg
      241 acgtccgggc agagtcggcg catgatgctc agcacgttgc gccgcacgtc gtcgacgcgg
      301 tcggtggtca acccgtcctc gatcagcttg cccacgtaga catcgtcgcc cttgcggcac
      361 atcatcagga aggtcgagtc gtcgctcgac acgtagcgtc gctccggacc gctgagcgcc
      421 tcgatttgtc ggcgcaggct tccgtcgatc tggaatccca cgaacagcgg gtgctcggtc
      481 gacagctcca cggtcattcc tcgaggagcg cgagcacttc gtcggtcttc gctcgcatca
      541 gcgccgggtc accgcgcgat tcgacgttca accggatcag cggctcggtg ttggacaggc
      601 gcaggttgaa ccgccagcgt tcgaaatcga gcccgatgcc gtcggtctcg tcgacccgga
      661 gggcgtcgcg ggaatagtgg cgacggacac gatccaacgc gcccgccggg tcggccagcc
      721 ggcggttgat ctctccgctg gccggatagc gttgcattct ctcgccgacc agaccgctga
      781 gcggtcgttg cgcacggcag atcagctcgg ccaccagcaa ccacggcacc atgccgctgt
      841 cgcagtagga gaagtcgcgg aagaagtggt gcgccgacat ctccccgccg tagaccgcgt
      901 cttgctcgcg catcacctgc ttgacgaagg cgtgaccgct cttcgactgg atcggtacgc
      961 caccggcctc gcggacgacc tcgatcgtgt tccacgtcag ccgcggatcg tgcacgacgc
     1021 gcgcgcccgg attcttcgcc aggatcgacg ccgccaacag gccgacgatg tagtaaccct
     1081 cgatgaagct ccccttctcg tcgaacagga agcaccggtc gaagtcgccg tcccacgcca
     1141 cgccgaagtc ggccccgctc tcgcgcaccg cccgcgacgt cgtctcgcgg ttctcttgca
     1201 ggagggggtt cggcacgccg ttggggaagg tcccgtcggg ctcgtggccc agcttgacga
     1261 agcggaacgg caggcgcggc tcgagcaggt cgacgatccg accggcgccg ccgtttccgg
     1321 cgttgacgac gatggtcagc ggttcgagcg cgccggtgtc gacgtaaccc gtcaggtggt
     1381 cgacgaacgc ggcgctgtcg tcggccgagg tcgtccgtcc cccgccgggc tcggggccca
     1441 gctcgtcgtc gaggatcatc cgttgcatct cgagcagccc ggtgtcggag ctgatcgggc
     1501 gcgcctgctc gcgaaccaac ttgagaccgt tgtagtcctt cgggttgtgg ctggcggtga
     1561 ccatgatccc gccgtcgagc ttcagctgga acgtggaaaa atagacctgc tcggtcccgc
     1621 actgcccgat gtcgacgacg tcgacgccgg ccgcgttcag cccgtcgatc aacgctccgg
     1681 cgatctccgg gctcgacaag cggatgtcgt ggccgacgac gacccgtttc ggtccgacga
     1741 accgcgcgta ggcgcggccg accttgtagg ccagctcgcg gtcgagctcg tccggcacgc
     1801 gcccgcggat gtcgtacgct ttgaagctcg ccagcccggc cggttgcccg cccgccatcg
     1861 atcgaccctc caggcatcgg agaatagcag aagcccgctg gccgcgacac ctcgcgagcg
     1921 tcacaccgct gcgatatgct gcccgcggca ccgaggaggc gacgatgagc gcggacgaga
     1981 cacggatcga gcgggactcg atgggcgaga tggaggtacc gcgagacgcc tactacggcg
     2041 cgagcacgca acgtgccgtt ctgaacttcc cgatcagcgg gctccgtatt ccgtcgccga
     2101 tgattcgcgc gctggggttg ctgaaatggg cggcggcccg ggtcaacgcc gagctcggcc
     2161 gtctcgatcc gcggctggcc gaggcgatcg cccgcgcggc gcgggaagtt gccgacggca
     2221 agctcgacga cgcgttcgtc gtcgacctgt tccagaccgg ctcggggacc tcgtccaaca
     2281 tgaacgccaa cgaggtcatc gccaatcggg cggccgagct gctcggcgcc ggtcgcggtg
     2341 cccgcgccgt gctccacccc aacgatcacg tcaacctggg ccaatcgtcg aacgatatca
     2401 tcccgacggc ggtccacctc ggcgcgctgc tggcgatcaa cgaatcgctc gaccccggcc
     2461 tcgcgctgct gcgcggcgcg ctcgagcgca agtccgagga gctgtggtct acgatcaaga
     2521 ccgggcgcac gcacttgcag gacgcgacgc cgatccggat ggggcaggtc tttcgcggct
     2581 acgccggcca gctcgaacgc gccgagcgac gcgtcgaatg ggccaagcag cagctcggcg
     2641 aggtcgcgct gggcggcact gcggtcggca ccgggatcaa cacgcatccg gagttcgccg
     2701 cgcgcgtctg cgcgatcgtc tcggccgact gcgcggtttc gatccacgag accgacaatc
     2761 acttccaggc gcaggcgacg ctcgacagcg tcgtcgccgc gtcgggcgcg ttgcgcacgg
     2821 tggcgatcag cctgctgaag atcgcgaacg acgtacgcct gttggcgtcc gggccgcgca
     2881 ccggtctgcg cgaactgacg attcccgagg tgcagccggg cagctcgatc atgccgggca
     2941 aggtcaatcc ggtgatcgcc gagtcgttga tccaggcgtc ggcccaggcg ctggccaacg
     3001 accaggcgat cgtgcaagcc ggcgagtgga gcttcttcga gctcaacacg atgctgccgc
     3061 tggccgg
//
LOCUS       DASPGN010000537         6542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1387417, whole genome shotgun sequence.
ACCESSION   DASPGN010000537 DASPGN010000000
VERSION     DASPGN010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387417"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1434)
                     /locus_tag="VD788_15615"
     CDS             complement(130..1434)
                     /locus_tag="VD788_15615"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV77741.1"
                     /translation="MKLRGGVVGCGVRLALVAVALPSAVALAAERTPAEPALDLAALV
                     AEAEAANPMLRAAAERRTGAQHGPAEAGALPDPTVSFSFQNESTDWTLGESPMSNLTF
                     AWSQEMPWGGKRRLAGDVARGEVGLLAAEEADLRAEVRARVKAAYVDLLRVDRTLAIL
                     EENRRLLAALREVAQARYEAGDALLENVLRAGAEISRLDLDIAELSGERWMVEADLGA
                     LLGRVERARFGRAVELPAVVDWDPAELEAVAAESSTRLARAEAQIALEARRLAAARRE
                     TKADWSWRAGYAERGGLDSIWSGGIAFRLPLYAKNKQREAIVRSEHDVEAARQEAVDV
                     EARVLGELRRLIVRVEVAELRRRTLEQAVMPQAQAAFDAARVAYESGQADFGTVFDAF
                     DRTLEDARLLERVRAEHLGGLARLEPLVGRELVLTGDGEDRE"
     gene            complement(1431..1916)
                     /locus_tag="VD788_15620"
     CDS             complement(1431..1916)
                     /locus_tag="VD788_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77742.1"
                     /translation="MERTNLRRAVPVAVLVAALATVACAPTERSCSQCGREECKNLAF
                     SVVSPGHVERTCCPRCGLRYLRETEPRVTRLEVRSFDDGAALDARTAFYVEASDVHPC
                     AHPAGSTPPVDERGCCLKAVYDRCEPSLVAFATRRDAERFAGEHGGFVRSWSDLETAV
                     R"
     gene            complement(2066..3661)
                     /locus_tag="VD788_15625"
     CDS             complement(2066..3661)
                     /locus_tag="VD788_15625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HXV77743.1"
                     /translation="MSITRRDFLTTAAIASAAGFVRLRAETPAGGRRVFLHGVASGDP
                     LADRVILWTRVTAPPGSSPAVAWELAADPAFRRVVTGGSVPTGDSRDFTVKIDAGPLD
                     PATSYYYRFEALGERSPVGRTRTLPAALAPRIRLALASCSNLPFGYFNVYARIAARSD
                     LDAVLHLGDYIYEYENGRYGDGTALGRVPEPDRELVSLADYRARYAQYRRDADLQEVH
                     RQHPFIVVWDDHEVTNNTWRDGADNHQPDLGEGDWSVRKAAAVRAYLEWMPIRADRAT
                     RRPRIYRSFAFGDLADLLMLDTRVLDRDRQAASRDAVAVIDDPARTLLGAAQEQWLVE
                     ELVASRRAAVQWQLLGQQVMFAPGSLPGAKAASVDTWDGYRPARDRLMTALAANDVRT
                     AIVLTGDIHSSWAYDLPRDPWSGYDATTGRGSLGVEIVVPAVTSPSGLGPPDEAAERI
                     ERLKRERPHLKFLEASRRGYVVLDVTRGRVQADWFFVPTVTERVPDEEFGGAWLTEAG
                     DPHLIAAATPAPPGPIGAEPAPL"
     gene            complement(3688..4938)
                     /locus_tag="VD788_15630"
     CDS             complement(3688..4938)
                     /locus_tag="VD788_15630"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXV77744.1"
                     /translation="MKHGTIAGAALLALALFGCARSERPAAAASPEALGFSSERLDRI
                     RQLLRSEVEQRHIGSAVALIARDGKIALVDAVGLADDETPMFDDAIMRLASAGKTFTA
                     TAIMILYERGALNLDDPVSKFIPAFSASRVSETDPRSGEQRLVAPKRPVEVRDLLTHT
                     AGLDISSEAFEQAWNEVTNDPSATTRDLAERIARLPLRTHPGEVFEYGSYGSCYEVLA
                     AIVEQASGTTLEQFLDDHLFGPLGMQDSHFWVPEEKQARLARIYRTRNGELSVDRERG
                     DETRRTSYIAGGGGVRSTVRDLFRFAQMLLDGGEIDGVRILSPKTVSLMMTNHAGNLV
                     PYGDGEFGWGFGGEVRLRVLEHGIGSVGTFGWAGGSGAKYWIDPQEKIVAVVVAPTAP
                     PAHWELMEKFERGMYAALTTSAAR"
     gene            5113..6174
                     /locus_tag="VD788_15635"
     CDS             5113..6174
                     /locus_tag="VD788_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HXV77745.1"
                     /translation="MNGTRWLAACVTRATLAAVLLAEASGTARASDHSDAPILGSVTR
                     VDANITDLHVFVVGPNLVLALSTNPAIPPSATSYVFPSDVTFELNIDVDSPVSPVDLV
                     GDGGTVLEPGRIHEEITFRVRFKEDGSPIVQRVQRAARTWATPEPTTLTSSRSDRNPP
                     AQFAPRSDGGDDESGFGWWSRRTESRSGRGTPLSRLGIASFFAGLRDDPFIRTPRAGR
                     NVAAIVIEVPLDSIARDGGTWLVWATSQVEEMDGSHQEMAGRSLRSMFSEQNALNGLH
                     PRDHLRVAGMRPDVMIFDTARAAAYPNGRALADDVVDLACSLGGECRVANADAPFPTT
                     NDVAFLAEFPYLAPPHLPE"
     gene            6171..>6542
                     /locus_tag="VD788_15640"
     CDS             6171..>6542
                     /locus_tag="VD788_15640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77746.1"
                     /translation="MTPRLAGTWLLVVVSASPVAAAAPVDPGTGPDQRIAYYRARAGG
                     TGSYVAWARLGQAYLDRGRMTGREQDRVRAERCLARSLAYQPNLEALRGMAVLRLDQH
                     RFGEALELAAEAAAAAPEDVAT"
BASE COUNT          936 a         2368 c         2293 g          945 t
ORIGIN      
        1 gggtgcatcg ggcagatcca ccgttctccg cccggggatg cgggatcgcc cgtccctcgc
       61 tcccgggtcc cccaccaggc ggcgccggcg aacacgacga cggcggcgaa gacgccgatc
      121 gcgatcgttc tattcacggt cctctccgtc gcccgtgagc acgagctcgc gaccgaccaa
      181 gggctcgaga cgggcgagtc cgcccaggtg ctcggcccgc acccgttcga gcaggcgcgc
      241 gtcctcgagc gttcggtcga acgcgtcgaa gacggtgccg aagtcggcct ggccgctctc
      301 gtaggccacg cgggccgcgt cgaacgccgc ctgcgcctgg ggcatcaccg cctgctcgag
      361 cgtgcgccgc ctgagctcgg cgacctccac cctcacgatc agccgccgca gctcgccgag
      421 cacgcgcgct tcgacgtcga ccgcctcctg acgcgccgcc tcgacgtcgt gctccgagcg
      481 cacgatcgct tcgcgctgct tgttcttcgc gtagagcgga agacgaaagg cgatcccgcc
      541 ggaccagatc gagtcgaggc cgcctcgctc ggcgtagccc gcgcgccagc tccaatcggc
      601 cttggtctcg cgacgcgcgg cggcgagccg tcgtgcttcg agcgcgatct gcgcttcggc
      661 gcgggcgagg cgcgtcgagc tctcggccgc caccgcttcg agctccgccg gatcccagtc
      721 caccaccgcc ggcaactcga cggcccggcc gaaccgcgct cgctcgaccc gaccgagtag
      781 ggcgccgaga tcggcctcga ccatccaacg ctcgccggac agctcggcga tgtcgagatc
      841 gagtcgcgat atctccgcgc ccgcacgcag gacgttctcg agcaacgcgt cgccggcctc
      901 gtagcgcgcc tgagcgacct cgcgcagcgc ggcgagcagc cggcgattct cctccaggat
      961 ggcgagcgtc cgatcgacgc gcagcagatc gacgtaggcc gccttgaccc gtgcccgcac
     1021 ctcggcccgc aggtccgcct cttcggccgc gagcaacccg acctcgccac gggcgacgtc
     1081 gccggcgagg cggcgcttgc cgccccacgg catttcctgg ctccaggcga aggtcaggtt
     1141 cgacatcggc gactcgccga gcgtccagtc ggtcgattcg ttctggaacg agaacgagac
     1201 ggtcgggtcg ggcagcgccc cggcttctgc cggaccatgt tgggcgcccg tgcggcgctc
     1261 ggcggcggcg cgcagcatcg ggttggccgc ctcggcctcg gcgacgagcg cggcgagatc
     1321 gagcgcgggc tccgccggcg tccgttccgc ggccagtgcg accgcggacg gtagggcgac
     1381 ggcgacgagc gcgagccgaa cgccgcagcc gacgacgccg ccccgcagct tcatcggacc
     1441 gccgtctcga ggtcgctcca gctgcggacg aaaccgccgt gctcgccggc gaagcgctcg
     1501 gcgtcccgcc gcgtggcgaa ggcgacgagg ctcggctcgc agcggtcgta gaccgccttc
     1561 agacagcaac cgcgttcgtc gaccgggggg gtgctgccgg cgggatgcgc gcaggggtgc
     1621 acgtccgagg cctcgacgta gaacgcggta cgagcgtcga gcgccgcccc gtcgtcgaag
     1681 ctccggacct cgagccgggt cactcggggt tcggtttcgc gcaggtagcg cagaccgcag
     1741 cgcgggcaac aggtccgctc gacgtggccg ggcgagacga cgctgaaggc caggttcttg
     1801 cactcctcgc gcccgcactg cgagcacgac cgttcggtcg gcgcacacgc caccgtcgcc
     1861 agcgccgcca ccaggacggc gaccgggacg gcgcggcgca gattcgttcg ctccatggtc
     1921 ttctcccccg cgagcgggag gcgtaacgga gggcaactcg cgtgccaccc gaacgagtcc
     1981 gccttccgcg cggaaaggat cgccggcggc cgcgatcagt gtagcgcccg caacagcgac
     2041 tgttgcattt ccaacagccg ggagcctaaa gcggcgcggg ctcggcgccg atcgggccgg
     2101 gcggagcggg ggtggcagcc gcgatcagat ggggatcgcc cgcctcggtc agccacgccc
     2161 cgccgaactc ctcgtcgggc acccgctcgg tgacggtcgg cacgaagaac cagtcggcct
     2221 ggacccgtcc ccgcgtgacg tcgagcacca cgtatccgcg gcggctcgcc tcgagaaact
     2281 tgaggtgcgg tcgctcccgt ttcagtcgct cgatgcgctc ggccgcctcg tcgggcggac
     2341 cgagcccgct cggcgaggtg acggccggca cgacgatctc gacgccgagc gagccgcgtc
     2401 cggtcgtcgc gtcgtagccg gaccacggat cgcgcggaag atcgtaggcc caggagctgt
     2461 ggatgtcgcc cgtcagcacg atcgcggtcc gaacgtcgtt cgcggcgagc gcggtcatca
     2521 gccgatcgcg ggccggtcga tagccgtccc acgtgtcgac gctcgccgcc ttggcccccg
     2581 gcagcgagcc gggcgcgaac atgacctgct gcccgaggag ctgccactgg acggccgcgc
     2641 gccgggacgc gaccaattcc tcgacgagcc attgctcctg cgcggcgccg agaagcgtcc
     2701 gtgccggatc gtcgatcacg gcgaccgcgt cgcgcgaggc cgcctgccga tcgcgatcga
     2761 ggacccgcgt gtcgagcatc agcagatcgg ccagatcgcc gaaggcgaac gatcgataga
     2821 tgcgcggccg gcgcgtcgcg cggtccgctc ggatcggcat ccactcgagg taggcccgca
     2881 ccgccgccgc cttccgcacg ctccagtcgc cttcgcccag gtccggctgg tggttgtccg
     2941 cgccgtcgcg ccaggtgttg ttggtcacct cgtgatcgtc ccagacgacg atgaacgggt
     3001 gctggcggtg gacctcctgc agatccgcgt cccgccggta ctgcgcgtag cgcgcgcgat
     3061 agtcggcgag cgagacgagc tcccgatcgg gctcgggcac ccggccgagc gcggtgccgt
     3121 cgccgtatcg gccgttctcg tactcgtaga tgtaatcgcc gaggtgcagc acggcgtcga
     3181 gatcggagcg agcggcgatc cgcgcgtaga cgttgaagta gccgaacggg aggttcgagc
     3241 aggaggccag cgcgaggcgg atccgcgggg ctaaggcggc gggcagcgtg cgggtccggc
     3301 cgaccggcga gcgctcgccg agcgcctcga accggtagta gtagctggtc gcggggtcga
     3361 gcggtccggc gtcgatcttg acggtgaaat cccggctgtc accggtgggg acgctgccgc
     3421 ccgtcacgac gcgccggaac gccggatcgg cggcgagctc ccacgccacc gcgggcgacg
     3481 agcccggcgg cgcggtcacc cgggtccaga ggatgacgcg gtccgcgagc ggatcgccgc
     3541 tcgcgacgcc gtggaggaac acgcggcgcc cgccggccgg cgtctcggcg cggagacgga
     3601 cgaaacccgc ggcgctcgcg atcgcggcgg tggtcaggaa atcgcgacgt gtgatcgaca
     3661 ctcgatagcc tcccgccgcc ctccgtctca gcgcgccgcg gaggtcgtga gcgcggcgta
     3721 cattccacgt tcgaactttt ccatcagctc ccagtgtgcc ggtggcgcgg tcggcgcgac
     3781 gacgacagcc acgatcttct cctgcggatc gatccagtac ttcgcgcccg acccgccggc
     3841 ccagccgaag gtcccgacgc tgccgatgcc gtgctcgagc acgcgcaacc ggacctcgcc
     3901 gccgaagccc caaccgaact cgccgtcgcc gtagggcacg aggttgccgg catgattcgt
     3961 catcatcaac gacacggtct tgggactgag gatccgcacg ccgtcgatct cgccaccgtc
     4021 gaggagcatc tgcgcgaagc ggaacaggtc gcgaacggtc gaccgcacgc cgccgccgcc
     4081 cgcgatgtag ctggttcgcc gcgtttcgtc gccgcgctcc cgatcgaccg acaactcgcc
     4141 gttccgcgtg cggtagattc gcgcgaggcg cgcttgcttt tcctccggca cccagaagtg
     4201 gctgtcctgc atcccgagcg gcccgaacag atggtcgtcg aggaactgct cgagcgtggt
     4261 cccgctcgcc tgttcgacga tcgccgccag cacctcgtag cacgatccgt aggacccgta
     4321 ctcgaacacc tcccccggat gcgtacgaag cggcaggcgt gcgatgcgct cggcgagatc
     4381 gcgcgtcgtc gccgacggat cgttcgtcac ctcgttccac gcttgctcga acgcctcgct
     4441 cgagatgtcg agacccgcgg tatgcgtcag caggtcgcga acctcgaccg ggcgcttcgg
     4501 agcgacgagt cgttgctcgc cggagcgcgg atcggtctcg gacacccgcg atgcggaaaa
     4561 cgcgggaatg aacttgctga ccggatcgtc gagattcagg gcgccgcgct cgtagaggat
     4621 catgatcgcc gtggcggtga aggtcttgcc ggcggacgcc aggcgcatga tcgcgtcgtc
     4681 gaacatcggg gtctcgtcgt ccgccaagcc gaccgcgtcg acgagtgcga tcttcccatc
     4741 ccgcgcgatc agcgcgacgg ccgagccgat gtggcgttgc tccacctcgg accgcagcag
     4801 ctgacggatg cggtccaagc gctccgagga aaagccgagg gcctccgggc tggctgcggc
     4861 ggcgggccgc tcggagcgcg cgcagccgaa cagcgccaac gcgaggagcg ccgcgccggc
     4921 gatcgtcccg tgcttcatcc gcccacctcc cgcgtactgg cggccgaccg tccgggtcgg
     4981 cggggtctgc tgaccctgcg actatacgcg gaatggcgcg tgtcggttcg gccccccggg
     5041 tggagcttga ctcgcgccgc tttcgcggta cattccgagc cgacgtccgg gttcccggac
     5101 aggagtccct cgatgaacgg cacccgttgg ttggcggcgt gcgtgacacg ggcaacgttg
     5161 gcggcggtgc tgctcgccga agcgtcgggc acggctcgtg cgtccgacca ttcggacgca
     5221 ccgatcctgg gcagcgtgac ccgcgtcgac gcgaacatca ccgatctcca cgtcttcgtc
     5281 gtcggaccga acctcgtact ggcgctctcg accaatccgg ccatcccgcc gtcggcgacg
     5341 agctatgtct tcccgtcgga cgtcacgttc gagctgaaca tcgacgtcga ttctccggtc
     5401 agtccggtcg atctcgtcgg cgacggcggc accgtgctcg agcccggccg catccacgaa
     5461 gagatcacct ttcgcgttcg cttcaaagaa gacggctcac cgatcgtcca gcgcgtgcaa
     5521 cgcgccgctc gtacgtgggc gacccccgag ccgaccacgc tgacctcgag tcgctcggat
     5581 cgcaacccgc cggcgcagtt cgcgccgcgg agcgacggcg gtgacgatga gtcgggcttc
     5641 ggttggtggt cgcgccggac cgagtcgcgc tccggacggg gcacgccgct gtcgcgcctg
     5701 gggatcgcga gcttcttcgc cggcctgcgc gacgatccgt tcatccggac tccgcgcgcg
     5761 ggtcgcaacg tcgccgcgat cgtgatcgag gtgccgctcg acagcatcgc ccgcgacggc
     5821 ggcacgtggc tggtttgggc gacgagccag gtggaggaga tggacggctc gcatcaggag
     5881 atggccgggc gctcgctgcg ctcgatgttc agcgagcaga atgcgttgaa cggcctgcac
     5941 ccgcgggacc acctgcgggt ggcggggatg cgccccgacg tgatgatctt cgacaccgcg
     6001 cgggccgcgg cctaccccaa cgggcgcgcg ctagccgacg acgtcgtcga cctcgcgtgc
     6061 agcctgggtg gggagtgtcg ggtcgcgaac gcggacgccc cgttcccgac gacgaacgac
     6121 gtcgcgttcc tcgccgagtt cccgtacctc gcgcctccgc atctgcccga gtgacgcctc
     6181 gcctcgccgg aacctggttg ctcgtcgttg tctccgcgtc gccggtcgcg gccgccgcac
     6241 cggtcgatcc cggcaccggg ccggatcagc ggatcgcata ctatcgggcg cgagccgggg
     6301 gtacgggcag ctacgtggcg tgggcccggc tcggtcaggc ctacctcgat cgcggtcgta
     6361 tgaccggacg cgagcaggac cgtgttcgcg ccgagcggtg tctcgcccgc tcgctggcct
     6421 accaaccgaa tctcgaggcg ctgcgcggga tggcggtctt gaggctcgac cagcatcgct
     6481 tcggcgaggc cctcgagttg gcggcggagg ccgcggccgc cgcgcccgag gacgtggcca
     6541 cg
//
LOCUS       DASPGN010000538         8998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_686328, whole genome shotgun sequence.
ACCESSION   DASPGN010000538 DASPGN010000000
VERSION     DASPGN010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8998
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686328"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..6126
                     /locus_tag="VD788_15645"
     CDS             10..6126
                     /locus_tag="VD788_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77747.1"
                     /translation="MRQAGTLGRGSANTPDSPIDNLWRSGMTNSPAVDEDEPIEYAVH
                     PEKFAGATIDPRRLPRIEVETKKSTGPPSDYHNSGLTFSEYNRLKELVQQAPPTIDGQ
                     EAVESGLRAPTPLGVSFDAIVSTQVAVPPDPIMAVGPNHLIALVNSRYRVWNKSGTPL
                     TSEITFNTLFSTVSNCQGSFDPFVDYDEAEDRFVIGAETILGNGNSYLCVAATATNDP
                     TGAWNVYSIRGDSLATSTVIDYPHMAIGQDAVYVAANMFNESGGFSHIRLFALAKSAL
                     YTGAAVTMAEANLGSAFFTGQPAKLHGFTSGGWPAPGAPHHIVAHNNNGSTRIWRWVN
                     PFVQAPTTYGTVSESFGGAPPNAPELGGGLLNDTNAGRYYDAEYRDGKVWTTRNVLCN
                     LGGGTSESCVDWLAIDVSGGAPVVIEQQTGGAYGSTNDFRYYPDIAVDRNNNIAIGYT
                     KSSASTYTQVWVTGRETSDPPGTLQPEILIRAGLGNYTDGAGCNGTCDRFGDYSGMTV
                     DPDGCTFWFIGEFSDGGIALWKTRIGNFKFDSCSVDSSVGVDKGSYTCDDTLTVTVTD
                     NIRIPAATVAAQTTITARDAAEMTIDSETILAGDWIGSDCVGSDCSIWRATLPVEGTT
                     GSDDDGTLNVVNGGTFVTFYDDPHPAHADQNRVSNVNCQTRFDDGGFLIDGGCELGTG
                     AEQYRDYLDGGEYIAYTYGLFNPPSAPPLTDVRVTLDVTGPAADAGLITVFNPTVEVG
                     GVGQGSLTGVVFNLFIDPAVDSATYRLSEHDFEVSITSPADGYTVAQLLSQTQFVQAD
                     DNIVSQSECFNFETDEGFVNTRYVFSYACSGADGCVPSRTVTTVEPPWTRGTGCQSEV
                     RTDVPSMSCDVGGTNANKTNATAGSCGNFAQSSNQLSDGITYTPIFGPANTGNAANGQ
                     PWNYQWLFAEWFFDSQMLSGTDVAIATGQFWDNDYVGSTTPATNEIDSLFPFFSGYFV
                     YPNQSWDSATPWDPLDPPANYDGTLFDDSASGLARSDLQWRWAFEGFDADFGTDPTAT
                     AATSGLAIDNLNLVYDQYHAEAQTGSCSGAAATISFDQLSYQTCGSGELEVTVLDDNA
                     GATVTVTVTSDDTGDSETLILGGGGPYYTTTLTYDTAGGAGVDDGVLFVTPDDIVRAS
                     YDDADPVTTVATGAFVECRGGDVVVDGVAGLADNGDGDSFGDTNEIVNLSLRLRNNSG
                     QELTNVTAVIASDSPNIDCISKDTASFGTLPAGGTGSNDLAGDPFVFKVAPTAQCSNP
                     SFPPTAVFTVFIEADDLDGSSLAQKLTMRLDLNDVGASTVYLEEFDTSPTDFTHQLGP
                     GDDDGNEVSPDGNPCSPYVDEAFWRSTGGNPGGGFFVWTNPAANFPFGAYSDLLDSEI
                     RSATITLPAGATAATLLFDHEYKFDSTDTLLPDGARVDYRVNGGPWQKLTTLPYDGSL
                     IFNSYCNPLCNNFGELECFSENPSSGENIFALLSTGTQTWKRVQGEVTGLAAGDELQF
                     RWRVGSMNLTFFGFPRIGGYGLDNVRVDATQQECDASVWPDEGCGVVFDDASNLVQLC
                     GNGNDVVEPTEIWAVDVDLKNVGSATAVNTTAELAVNPGSPNGVVVTGSPGNFGSIAA
                     EGGIGTASFEFEVDGTAVCVDDIVFDVTNIADSLRSYGDVVAAFAVEVGELLANQSAF
                     QAIDPIVANAGSAESAMSPNLALTLPVAGATLSYDFNYTNIAPTENAFQDNDITGVVN
                     AIGVSTMSPAFTIAAGSAASASVFWELLTYPGGNLDQCTRVFLRTPNGTDVTLKALND
                     PPSTPYNVLPTYQGANGGPGQYQIGLEERQGGGCNGAANLSSTTMIVTQAPGSASWTA
                     NAQVSLSHGGSTTVVKPYGVPDANPYDVGSLYAGPGTYTVIVEENGAGGDARLSSAEL
                     FVFDAQCAMGCSGVLPAPPPVADGATGEFMTLDPGVGPGELTIDIDNATCSGDHAVVL
                     YGTIGDYSGYQGAVDLGCDIGDGQSATFTHAGDNVWFNVIWVNANDVAGSPGLASAGQ
                     RTWSADGLCNVVAEDTADNTCN"
     gene            6228..7079
                     /locus_tag="VD788_15650"
     CDS             6228..7079
                     /locus_tag="VD788_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77748.1"
                     /translation="MRGLHSLPVVLGVLAGLVVAGSARAQKSYDDHELTDRLYFRIGG
                     FQQTEIRTTLRIDAMTSQGAIAAGAVVALESLFKVDDSVSTVRFDGWYRLGKKSRLNW
                     TVWQTDRDGINTYEGDPLTVGDILIESGQMVRIRDKTTLYAFSYTYSFLNTEKYEAWL
                     GGGFNFQRVETTIESTLGGSTFEQMEDAKATVPIPVLSFGGRWNLSRRWRTLITQELF
                     GIDIGDYSGRLSNTRILAEVNLTRNFGLGAGLERYNFQVDAEADDFTGSFDTGYTGLS
                     VYLKGQL"
     gene            7091..7927
                     /locus_tag="VD788_15655"
     CDS             7091..7927
                     /locus_tag="VD788_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77749.1"
                     /translation="MRATLCLALVVLAGPRATAAGDDARPIIERGFAAAEANAVLERL
                     YVYHQTIEERHFGSKGREKKRESRTWDVTRLDGSEYRRLIAQNGRPLDEHEQAAEQRR
                     LEKQLDKMRRETPSQRAQRLRSVEREHEESRGFFEQVTRAFDFQLLREEPLDGVPCYV
                     IAAEPRADYEPVSRETRVLTKVRATLWISQSDSVWVRAEIETIESFTWAMVLRVKPGA
                     QVQFSRRRVNDEVWLPHTWYLRYKGKAAGIFGFNREFSASYDNYRKFTAESSGVFDGG
                     AR"
     gene            complement(7955..8533)
                     /locus_tag="VD788_15660"
     CDS             complement(7955..8533)
                     /locus_tag="VD788_15660"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HXV77750.1"
                     /translation="MKKRISILALLVALCLGCASDPTAPENGTVAVLLTDAPIDLSTV
                     SAVEVTLDRIVLFAEDGMVEDQQGMEMEQPGVAGGAGMTLNLLDYQNGQTVLIATLEV
                     PAGQYQKIRMYVRNAELVETDPADGTTEIRHEITVPSSKVDVPVAFTVSGGESTEVVL
                     DFDAALSVQVNETAGNPEYILRPVITPTGVTG"
     gene            complement(8659..>8998)
                     /locus_tag="VD788_15665"
     CDS             complement(8659..>8998)
                     /locus_tag="VD788_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77751.1"
                     /translation="DRVRAALADDGTRGVFRLARAAQGDHAAARYLRELVRQRELDPD
                     QLLTVLQLAAERVDNTAECQGLLASIVKRYDLDTPVLRAAFARAKARLGPRAAIDTFG
                     RRSDTRAASR"
BASE COUNT         1593 a         3028 c         2961 g         1416 t
ORIGIN      
        1 gagcaacggt tgcgccaggc cggcacgctc ggccggggct ccgccaacac gcccgactcg
       61 ccgatcgaca acctgtggcg ctccgggatg accaactctc cggccgtcga cgaggacgag
      121 ccgatcgagt acgcggtcca ccccgagaag ttcgcgggag cgacgatcga tccccggcgc
      181 ctgccgcgga tcgaggtcga aacgaagaaa tcgaccggcc cgccgtcgga ctatcacaac
      241 tccgggttga ccttcagcga atacaatcgg ttgaaggagc tggtgcagca ggcgccgccg
      301 acgatcgacg gccaggaggc ggtcgagagc gggttacgcg cgccgactcc gctcggtgtc
      361 tcgttcgacg caatcgtctc gactcaggtc gccgtgccgc ctgacccgat catggcggtc
      421 ggcccgaacc acctgatcgc gctggtcaac tcccgttatc gcgtctggaa caagtccggc
      481 acgccgttga cgtccgagat caccttcaac acgctgttct cgaccgtttc gaactgtcag
      541 ggctcgttcg acccgttcgt cgactacgac gaggccgaag accgcttcgt gatcggcgcc
      601 gagacgatcc tcggcaacgg caactcgtac ctgtgcgtgg cggcaacggc gaccaacgac
      661 ccgacgggag cgtggaacgt ctattcgatc cgtggcgact cgctcgcgac ctcgaccgtg
      721 atcgactacc cgcacatggc gatcggtcag gacgcggttt acgtcgcggc caacatgttc
      781 aacgagagcg gcggcttctc ccacatccgc ctgttcgcgc tcgccaagtc ggcgttgtac
      841 accggggcgg cggtgacgat ggccgaggcc aacctcggca gcgcgttctt caccggacaa
      901 cccgccaagc tccacggatt cacgtcgggt ggttggcccg cgccgggcgc gccgcatcac
      961 atcgtcgcgc acaacaacaa cggctcgacc cgcatctggc gctgggtcaa tccgttcgtc
     1021 caggcgccga cgacctacgg caccgtctcc gaatccttcg gcggcgcgcc accgaacgcg
     1081 cccgagctgg gcggaggcct gctgaacgac accaacgccg gccgctatta cgacgccgag
     1141 tatcgcgacg gcaaggtctg gaccacgcgc aacgtgctgt gcaacctcgg cggcggcacc
     1201 agcgagtcct gcgtcgactg gctggcgatc gacgtgtcgg ggggcgctcc ggtcgtcatc
     1261 gagcagcaga cgggcggcgc ttacggatcg accaacgatt tccgctacta tcccgacata
     1321 gcggtcgacc gcaacaacaa catcgcgatc ggctatacca aatcgagcgc gtcgacctac
     1381 acccaggtgt gggtcaccgg ccgcgagacg agcgacccgc ccggcacgct ccaacccgag
     1441 atcctgatcc gtgccggact cggcaactac accgacggcg cgggctgcaa cggcacctgc
     1501 gaccgcttcg gtgactactc gggcatgacc gtcgaccccg acggttgcac cttctggttc
     1561 atcggcgagt tctcggatgg cggaatcgcg ttgtggaaga cgaggatcgg gaacttcaag
     1621 ttcgacagct gcagcgtcga ctcgtcggtc ggcgtggaca agggctcgta cacctgcgac
     1681 gacacgctga ccgtgacggt gacggacaac atccgcatac cggccgcgac ggtcgcggca
     1741 cagacgacga tcaccgcccg ggacgcggcg gaaatgacga tcgacagcga gacgatcctc
     1801 gccggcgact ggatcggcag cgactgtgtc ggctcggact gctcgatctg gcgggcgacg
     1861 ctgccagtcg agggcacgac cggcagcgat gacgacggta cgctcaacgt cgtcaacggc
     1921 gggacgttcg tcacgttcta tgacgacccg catccggcgc acgccgacca gaaccgcgtg
     1981 tcgaacgtca actgccagac gcggttcgac gatggcggct tcctgatcga cggcggttgc
     2041 gagttgggca ccggcgccga gcagtatcgc gactacctgg acggcggcga gtacatcgcc
     2101 tacacctacg gcctgttcaa tccgccctcg gcgccgccgt tgaccgacgt acgggtcacg
     2161 ctcgacgtga cggggccggc ggccgacgcc ggtctgatta ccgtgttcaa tccgaccgtc
     2221 gaggtcggtg gagtcggcca ggggagcctg acgggcgtcg tgttcaacct gttcatcgac
     2281 cccgccgtcg acagcgcgac ctaccgactg agcgaacacg acttcgaggt gagcatcacc
     2341 tcacccgccg acggctacac cgttgcccag ctgttgagcc agacgcagtt cgtgcaggcg
     2401 gacgacaaca tcgtcagcca atcggagtgc ttcaacttcg agaccgacga ggggttcgtc
     2461 aatacgcgct acgtcttcag ctatgcctgt agtggggcgg acggttgcgt gccctcgcga
     2521 accgtgacca cggtcgagcc gccgtggacc cgcggcaccg gctgtcagag cgaggtccgc
     2581 accgacgtac cgagcatgag ctgcgacgtc ggcggaacca acgccaacaa gacgaacgcc
     2641 accgccggca gctgtggcaa cttcgcccag tcgagcaacc agttgtccga cggcatcacc
     2701 tacacgccga tcttcggacc ggccaatacc ggcaatgcgg ccaacggcca gccgtggaac
     2761 taccagtggc tgttcgccga gtggttcttc gactcgcaga tgctatccgg gaccgacgtg
     2821 gcgatcgcca ccggccagtt ctgggacaac gactacgtcg gctcgacgac gccggcgacc
     2881 aacgagatcg attcgttgtt cccgttcttc tccggctact tcgtctatcc caaccagagc
     2941 tgggacagcg cgaccccgtg ggacccgctg gacccgcctg ccaactacga cgggacgcta
     3001 ttcgacgatt cggcctccgg cctcgcccgt tcggatctgc agtggcgctg ggcgttcgag
     3061 ggcttcgatg ccgatttcgg taccgatccg acggccaccg ccgcgacgag cggcctcgcc
     3121 atcgacaatc tcaacctggt ctacgaccaa taccacgcgg aagcccaaac cggcagctgc
     3181 agcggcgccg cggcgacgat ttcgttcgac cagctgagct accagacctg cggcagcggt
     3241 gagctcgagg ttaccgtgct cgacgacaac gccggcgcca cggtgacggt cacggtgaca
     3301 tcggacgaca ccggcgacag cgagacgctg atcctcggcg gcggcgggcc gtactacacg
     3361 acgacgctga cctacgacac ggcaggcggc gccggggtcg acgacggcgt gctgttcgtc
     3421 accccggacg acatcgttcg ggccagctac gacgacgccg atccggtcac gaccgtagcg
     3481 accggtgcgt tcgtcgaatg tcgcggcggt gacgtcgtcg tcgacggcgt cgccggtctg
     3541 gccgacaacg gcgacggcga cagtttcggc gacaccaacg agatcgtcaa cctgtcgctg
     3601 cggctgcgca acaactcggg gcaggagctg accaacgtga ccgcggtcat cgccagtgac
     3661 agcccgaaca tcgactgcat ctcgaaggac acggccagct tcggcacgct gccggcaggt
     3721 ggcaccggct cgaacgacct ggccggcgac ccgttcgtgt tcaaggtggc accgacggcg
     3781 cagtgctcga atccatcgtt cccaccgacg gccgtgttca ccgtcttcat cgaggccgac
     3841 gatctcgacg gctcgtcgtt ggcgcagaag ctgaccatgc ggttggacct caacgacgtc
     3901 ggtgcgtcga cggtctacct cgaggagttc gacacctcgc cgaccgactt cacccaccag
     3961 ttgggaccag gcgacgacga cggcaacgag gtgagcccgg acggcaaccc gtgctcgcct
     4021 tacgtcgacg aagcgttctg gcgctccacc ggcggcaatc ccggcggcgg tttcttcgtc
     4081 tggaccaacc cggcggccaa cttccccttc ggcgcgtaca gcgatctgct cgacagcgag
     4141 attcgctccg cgacgatcac gctgccggcc ggagcgacgg cggcaacgtt gttattcgac
     4201 cacgagtaca agttcgactc gaccgacacg ttgttgcccg acggcgctcg cgtggactac
     4261 cgcgtcaacg gtggtccgtg gcagaagctg accacgctgc cctacgatgg ctcgttgatc
     4321 ttcaacagct actgcaaccc gctgtgcaac aacttcggcg agctcgagtg cttctccgag
     4381 aacccgtcgt cgggcgagaa catcttcgcc ctgttgagca ccggcacgca gacctggaaa
     4441 cgggtccagg gtgaagtcac cgggctcgcc gcgggtgacg agctgcagtt ccgctggcgg
     4501 gtcggctcga tgaacctgac gttcttcggc ttcccgcgca tcggtggata cggcctggac
     4561 aacgtccgcg tcgacgccac gcagcaggag tgcgacgcca gcgtttggcc ggacgaggga
     4621 tgcggcgtcg tattcgacga cgcgagcaac ctcgtccagc tgtgcggcaa cggcaacgac
     4681 gtcgtcgaac cgaccgagat ctgggccgtc gacgtcgacc tcaagaacgt ggggtcggcc
     4741 accgcggtca acacgaccgc cgagctggcg gtcaatcccg ggtcgccgaa cggcgtggtg
     4801 gtgaccggca gtccgggcaa cttcggctcg atcgcggccg agggcgggat cggaacagcg
     4861 agcttcgagt tcgaggtcga cggaaccgcg gtctgcgtcg acgacatcgt cttcgacgtc
     4921 accaacatcg ccgacagcct gcgaagctac ggcgacgtcg tcgcggcgtt cgcggtcgag
     4981 gtgggtgagc tgctcgccaa tcagtccgcg ttccaggcga tcgacccgat cgtggccaac
     5041 gccggatcgg cggagtcggc gatgagcccc aacctcgcgt tgaccctgcc cgtcgccggc
     5101 gcgacgctga gctacgactt caactacacc aacatcgcgc cgacggagaa cgcgttccaa
     5161 gacaacgaca tcaccggtgt cgtcaacgcg atcggcgtat cgacgatgag cccggcgttc
     5221 accatcgcgg ccggctcggc agcgagcgcg agcgtgttct gggagctgct gacctacccc
     5281 ggtggcaacc tcgatcaatg cacgcgcgtc ttccttcgca cgccgaacgg caccgacgtc
     5341 acgctgaagg cgctcaacga tccgccgagc acgccctaca acgtcctgcc gacctaccag
     5401 ggcgccaacg gtggcccggg gcagtaccag atcgggctcg aggagcgaca gggtggcggt
     5461 tgcaacggcg cggcgaacct gtccagcacg acgatgatcg tgacccaggc acccggatcg
     5521 gcgagctgga cggccaacgc ccaggtcagc ctgtcccacg gtggctcgac gacggtggtc
     5581 aagccgtacg gcgtgcccga tgccaatccc tacgacgtcg gctcgctcta cgccggcccg
     5641 ggcacctaca cggtgatcgt cgaggagaac ggggcgggcg gcgacgccag gctcagctcg
     5701 gccgagctgt tcgtgttcga cgctcagtgc gcgatgggct gctcgggcgt gcttcccgcg
     5761 ccgcctccgg tcgccgacgg agcgaccggc gagttcatga cgctcgatcc gggggtcggg
     5821 ccgggcgagc tcaccatcga catcgacaac gccacctgct cgggcgatca cgcggtcgtg
     5881 ctctacggca cgatcggcga ctacagcggc taccagggcg cggtcgatct cggctgcgac
     5941 atcggtgacg gacagagcgc cacgttcacc cacgccggtg acaacgtctg gttcaacgtg
     6001 atctgggtca acgccaacga cgtcgccggc agcccgggtt tggccagcgc cggacagcga
     6061 acgtggtcgg ctgacggcct gtgcaacgtc gtcgcggagg acaccgccga caacacctgc
     6121 aactgaccgt cgttcgatcg aactcaacgg gccaggcgcc gccttgggcg gtgcctggcc
     6181 tttttcgtct gaatgttatg ctagggccgt cgtggggtga ggtccgcatg aggggtctcc
     6241 acagcctgcc ggtcgtcctc ggcgtcttgg ccggcttggt cgtcgcgggt tcggcccgcg
     6301 cgcagaaatc gtacgacgat cacgagttga cggaccgtct ttacttccgc atcggcggct
     6361 tccagcaaac cgagatccgg accacgttgc ggatcgacgc gatgacgtcg caaggcgcga
     6421 tcgcggccgg ggccgtggtc gcgctcgaga gcctgttcaa ggtggacgac agcgtctcga
     6481 cggtccggtt cgacggttgg taccggctgg gcaagaagag ccggctcaac tggaccgtgt
     6541 ggcaaaccga ccgtgacggc atcaacacct atgagggcga tccgctcacc gtcggcgaca
     6601 tcctgatcga gtcggggcag atggtacgga tccgcgacaa gacgacgctg tacgccttca
     6661 gctacaccta ctcgttcctc aacaccgaga agtacgaagc gtggctcggc gggggcttca
     6721 acttccagcg ggtcgaaacg acgatcgaat cgacgttggg tgggtcgacc ttcgagcaga
     6781 tggaagacgc caaggcgacg gtcccgatcc ccgtgctcag cttcggcggc cggtggaatc
     6841 tcagccgccg ctggcggacg ctgatcaccc aggagctgtt cggcatcgac atcggcgact
     6901 actccggccg gctgagcaac acacgcatcc tggccgaggt caacctgacg cggaacttcg
     6961 gtctcggcgc ggggctcgag cgctacaact ttcaggtgga cgccgaggcc gacgacttca
     7021 ccggctcgtt cgatacgggc tacaccgggt tgtcggtcta cctgaagggt cagctctgag
     7081 cggaccgccg gtgcgagcga cgctgtgcct ggcgctggtc gtgctggcgg ggccgcgcgc
     7141 gaccgccgcg ggcgacgacg cgcgaccgat catcgagcgc gggttcgccg cggccgaggc
     7201 caacgccgtg ctcgaacggc tttacgtcta ccatcagacg atcgaagaac gccacttcgg
     7261 cagcaagggg cgcgagaaga agcgggagtc ccgcacgtgg gacgtgacgc ggttggacgg
     7321 cagcgagtac cgtcgattga tcgcgcagaa cggccggccg ctcgacgagc acgagcaggc
     7381 cgccgagcaa cgccggctgg aaaagcagct cgacaagatg cgacgcgaaa ccccctctca
     7441 gcgagcgcag cggttgcgct cggtcgagcg ggaacacgag gagtcgcgcg ggttcttcga
     7501 acaggtgacg cgggcgttcg atttccagct gttgcgcgag gaaccactcg acggcgtgcc
     7561 gtgctacgtc atcgccgccg aaccgcgtgc ggactacgaa ccggtctccc gtgagacccg
     7621 cgtgctgacc aaggtccgcg cgacgttgtg gatctcgcag agcgactcgg tctgggtccg
     7681 cgccgagatc gagacgatcg agagtttcac ctgggcgatg gtgttgaggg tcaagcccgg
     7741 ggcgcaggtc cagttcagcc ggcgtcgggt caacgacgag gtgtggctgc cgcacacgtg
     7801 gtacttgcgc tacaagggca aagccgccgg gatcttcggc ttcaaccgcg agttcagcgc
     7861 gagctacgac aactaccgca agttcaccgc ggaatcgagc ggcgtgttcg acggcggagc
     7921 gcgctgacgg tcgctcaccg atcgatcgag catgctagcc ggtgactccg gtcggcgtga
     7981 tcaccggccg caggatgtat tccgggttgc ccgcggtttc gttgacctgc acgctcagcg
     8041 cggcatcgaa gtcgaggacg acttcggtgc tctccccacc cgagacggtg aaggcgaccg
     8101 gcacgtcgac cttgctcgac ggcacggtga tctcgtggcg gatctccgtc gtcccgtcgg
     8161 ccgggtcggt ttcgaccagt tccgcgttgc gcacgtacat gcggatcttc tggtactgtc
     8221 cggccggcac ttcgagcgtg gcgatcagca cggtctgacc gttctggtag tcgagcaggt
     8281 tgagcgtcat tcccgccccg cccgcgacac cgggctgctc catctccatg ccctgctggt
     8341 cttcgaccat cccgtcctcg gcaaacagca cgatccggtc gagcgtgacc tcgacggcgc
     8401 tgacggtcga cagatcgatc ggcgcgtcgg tcagcagcac cgcgacggta ccgttctccg
     8461 gtgcggtcgg gtcggacgcg catcccaggc agagggcgac gaggagcgcg agtatcgaga
     8521 ttcgcttctt catcggcaac gtcctccttt ggcgctcgca cgagcgctac ggggaacgat
     8581 gcaagccccg cgccggcagt agacggctgg aaaacgcccg gggacgggac gatcgggaga
     8641 cgatacggta atttccgctc accgggacgc ggcgcgcgtg tccgaccgtc gaccgaacgt
     8701 gtcgatcgcg gcgcgcgggc cgagccgcgc cttcgcgcgg gcgaacgcgg cgcgaagaac
     8761 cggcgtgtcg agatcgtatc gcttgacgat cgaggccaac agcccctggc attcggcggt
     8821 gttgtcgacc cgctcggcag cgagctgcag cacggtcagc agttggtccg ggtcgagctc
     8881 gcgctgccgg acgagctccc gcaggtaacg cgcggcggca tggtcgccct gggccgctcg
     8941 cgccagacgg aacacgccgc gcgtcccgtc gtcggcgagc gccgcgcgca cgcggtcg
//
LOCUS       DASPGN010000539         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1505668, whole genome shotgun sequence.
ACCESSION   DASPGN010000539 DASPGN010000000
VERSION     DASPGN010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1505668"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VD788_15670"
     CDS             complement(<1..992)
                     /locus_tag="VD788_15670"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXV77752.1"
                     /translation="MIDPGPRWPRLALILSALLATALDPASASDPATFEAPVKPPPPD
                     EPPRPQVVEPEAPLTLYDAIALTRMEDPELQDAAWAGRAAEARAAQAKKVPNPEVDLR
                     MWRLDGEARNEPDDLDRYRVIVSKEVEFGGRRRWRTDQARAEQEVAGWEFAAKRDEVS
                     GEVTIRFVEVLGAQRRVEVYRELGAFHEQLERTVAKLVETGSLRPVEIHQLRRRAGLA
                     RIDLARAEAELDASRMDLTLSWGNAAPRFTEAIGDLDRVPALPDRETVLALAEASPTA
                     ESWQARLDAGRAAISLAKSNAVPDVQLGVGARWLNEMNETDYMLDLEIELPIFDR"
     gene            complement(1095..2510)
                     /locus_tag="VD788_15675"
     CDS             complement(1095..2510)
                     /locus_tag="VD788_15675"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV77753.1"
                     /translation="MGNHRQRGLDVNLNLNVRGMTPSATVAINERCDALIREGRPVYK
                     LGLGQSPFPVPDPVVEELRAHAFQKDYLPVKGLWRLRRAVADYHRRTNGLTSTWQDVL
                     IGPGSKELMFLLQLVYYGDLVIPRPSWVSYAPQARIIGRQVRWLDTRRENNWRMMPAD
                     LERLCVEDPDRPRVVIVNYPSNPTGATYSTERLRELAQVARRFRVVLLSDEIYGELHH
                     KGQHVSLGRYYPEGTIVSSGLSKWCGAGGWRLGTFTFPPALGWLRDAMAAVASETFTS
                     TSAPIQYAAVRAFQGGTEIERYLWRSRKILGALGRWAAKRLAAAGVDVHPPAGAFYLF
                     PDFSAFASRLAERGITDSRGLCERLLEDAGVALLPGSDFGRPESELTARLAYVDFDGA
                     RALAALEAETQAGPLDDSFLRSHCARILEAIDRVTEWLGVERAARAAGAERGSDSQSE
                     PSERDPAARPDDLRSRGQGRA"
     gene            2620..>3102
                     /locus_tag="VD788_15680"
     CDS             2620..>3102
                     /locus_tag="VD788_15680"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HXV77754.1"
                     /translation="MMESARPARSVPLAVSARRTFYDEGLIVLRGVFRPDEVAEMRAA
                     FDRLEQLAKRLDGTEIHHGSQFVVERSDHHPLRIHRVVWCGGAEPVLGRYGEDPRLTG
                     LASRLLGSRRIVQLINQAHFKLPGDGVAFPWHQDSRHRRYGEEAWADVNGRGSYVQTV
                     T"
BASE COUNT          436 a         1140 c         1070 g          456 t
ORIGIN      
        1 cggtcgaaga tcggcagctc gatctcgagg tcgagcatgt agtcggtctc gttcatctcg
       61 ttcaaccagc gtgcgccgac gccgagctgc acgtcgggaa ccgcgttgga cttggcgagc
      121 gagatcgccg cccgaccggc gtcgagccgc gcctgccagc tctccgccgt cgggctcgcc
      181 tcggccagcg ccagcacggt ttcgcggtcc ggaagcgccg gcacgcggtc gagatctccg
      241 atcgcctcgg tgaagcgcgg cgcggcgttg ccccacgaca gcgtgaggtc catccgcgac
      301 gcgtcgagct ccgcctcggc ccgcgcgagg tcgatccgcg cgagcccggc ccgacggcgg
      361 agctggtgga tctcgaccgg tcgcagcgac ccggtctcga ccagcttggc gaccgtccgc
      421 tctagctgct cgtggaacgc gcccagctcg cggtagacct cgacgcgccg ttgggcgccg
      481 aggacctcga cgaagcggat cgtcacctca cccgacacct cgtcccgctt ggcggcgaac
      541 tcccagccgg cgacctcttg ctcggcacgc gcctggtcgg tgcgccagcg ccgccggccg
      601 ccgaactcga cctccttgct gacgatcacc cggtaccgat cgaggtcgtc gggctcgttg
      661 cgggcctcgc cgtccagacg ccacatacgc aggtcgacct ccgggttggg caccttcttc
      721 gcctgggccg cccgcgcctc ggcggcgcga ccggcccagg ccgcatcctg cagctcgggg
      781 tcttccatcc gcgtcagtgc gatcgcgtcg tacagcgtca gcggtgcctc gggctcgacc
      841 acctgcggtc gcggtggctc gtccgggggc ggcggcttga ccggcgcctc gaaggtcgcg
      901 ggatccgacg cgctcgccgg atcgagcgcc gtcgcgagca gcgcggacag gatgagcgcg
      961 agacgcggcc aacgtggacc gggatcgatc atcggttcgt cccctcgact ccatccgccg
     1021 gccgcggagc cggtaatcgg gccgattcta ctcgaatcct ccacacggac tgtacgatct
     1081 gtgcgcggtg tcggctaggc tcggccctgc ccccgcgagc ggaggtcgtc gggacgggcg
     1141 gcgggatcgc gctcggacgg ctcggactgc gagtcggacc ctcgctcggc gccggccgcg
     1201 cgcgccgctc gttcgacacc gagccactcg gtcacgcggt cgatcgcctc gaggatcctc
     1261 gcacagtggc tccgcaggaa ggaatcgtcg agcggtccgg cctgggtctc ggcctcgagc
     1321 gcggcgagcg cgcgcgcgcc gtcgaagtcg acgtaggcca gccgcgcggt caactcgctc
     1381 tccggccgac cgaagtcgct gcccggcagc agcgcgacac ccgcgtcctc gagcaacctc
     1441 tcgcacagcc cgcgcgagtc ggtgatgccg cgctcggcca accgcgaggc gaaggcggaa
     1501 aagtccggaa aaagatagaa cgcgccggcc ggtggatgga cgtcgacgcc ggccgcggcc
     1561 agccgcttgg ccgcccagcg ccccagcgcc ccgaggatct tgcgcgagcg ccacaggtag
     1621 cgctcgatct ccgtcccacc ctggaaggcc cgcacggccg cgtattggat cggcgccgaa
     1681 gtcgaggtga aggtctcgct cgccaccgcg gccatcgcgt cgcgcaacca gccgagcgcc
     1741 ggcgggaagg tgaaggtccc gagacgccag ccgcccgcgc cgcaccactt gctgagcccg
     1801 ctgctgacga tcgtaccctc ggggtagtac cggccgagcg agacgtgctg tcccttgtgg
     1861 tgcagctcgc cgtagatctc gtcggacagc agcacgacgc ggaaccggcg cgcgacctgg
     1921 gccagctcgc gcaggcgctc ggtgctgtag gtcgccccgg tcgggttgct cgggtagttg
     1981 acgatcacga cgcgcggccg gtcggggtcc tcgacgcaaa gccgctcgag atccgccggc
     2041 atcatccgcc agttgttctc gcgccgcgtg tcgagccagc gaacctgccg cccgatgatc
     2101 cgcgcctggg gcgcgtacga gacccaactg ggccgcggga tgacgagatc gccgtagtag
     2161 acgagctgga gcaggaacat cagctccttc gagccggggc cgatcaacac gtcctgccag
     2221 gtggaggtca gcccgttggt ccgccggtgg tagtccgcca ccgcccgccg cagtcgccac
     2281 agccctttga ccggcaggta gtccttctgg aacgcgtggg cgcgcagctc ctcgaccacc
     2341 gggtcgggaa cggggaacgg cgactggccc agcccgagct tgtagaccgg ccggccctca
     2401 cgaatcagcg cgtcgcatcg ctcgttgatc gcgaccgtgg ccgacggcgt catgccgcgc
     2461 acgttgaggt tcaggttgac gtccaggccg cgctgcctgt ggttccccat ccgcggctcc
     2521 tccgccgttc gacgggacca tcttaggctc gcgtggggcg ggatccaaat tcgcgatccg
     2581 ggcctgcacg gcgggccgac cgttcgtacc ttcgtcggga tgatggagtc cgcccgaccc
     2641 gcgcgctcgg ttccgctcgc cgtttcggcg cggcgcacgt tctacgacga ggggctgatc
     2701 gtcctccgcg gcgtgttccg ccccgacgaa gtcgccgaaa tgcgggcggc gttcgatcgc
     2761 ctcgagcagc tcgccaagcg tctcgacggg accgagatcc accacggctc gcagttcgtc
     2821 gtcgagcgct cggaccatca cccgctgcgc atccaccggg tggtctggtg cggaggcgcg
     2881 gagcccgtcc tcggaagata cggggaggac ccgcgtctga ccgggctcgc ctcgcgcctg
     2941 ctcggctcgc gccggatcgt ccagctgatc aaccaggccc acttcaagct ccccggcgac
     3001 ggcgtcgcct tcccctggca ccaggacagc cgtcaccggc gctacggcga agaagcgtgg
     3061 gccgacgtca acggtcgcgg cagctacgtg cagacggtca cg
//
LOCUS       DASPGN010000540        16421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1506534, whole genome shotgun sequence.
ACCESSION   DASPGN010000540 DASPGN010000000
VERSION     DASPGN010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 16421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16421
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506534"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2207
                     /locus_tag="VD788_15685"
     CDS             <1..2207
                     /locus_tag="VD788_15685"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HXV77755.1"
                     /translation="VERVQRALLSRLHRGGIATVDRIHALARHDAAGESAGDDRERER
                     RRMQQIVWDLASRAFPPNEIEDVLNLALKREEARTLEEIANLSAVSFGLIADKVRAFC
                     RLPKATSPLPEAEAQAVRVALIRHFISDQLEFIAVAKQQLTVRDFGELVDHVIGPEAG
                     IGRIGGKAAGLFLAERVLRRARQSDPAAPDIPIRCPESFYLRSDVIERFLDQAGLVEL
                     QSHKYKSIGEIRDEYPTIVDTFKSAEFPAEVSDQLGSMLQAVGPHPLIVRSSSLLEDR
                     FGTAFAGKYSSVFLTNQGPPAERLAHLLRAIAEVYASTLHADPISYRRKHKLLDYDEN
                     MAVLIQKVVGTSYRHYFLPAWAGVAFSRNPHRRNPRIRAEDGMARIAFGLGTRAVDRV
                     SGDCVRMVPLGMPTLRPEVTPEEICRQSQREVDLLNLRDHRFETILAGELLQEGEEFP
                     GLDQVVSVLDRGFLSRPVGTRVLAAPSDLVVTFDRWTHDSPYPRLLGWVLRTLERAYG
                     CPVDVEFAHDGREFHLLQCRPQVLRRAEQVRLPTDVAVQRRVFLADREIESGSVHGVE
                     FIVLIDPRDYGRQDSSERRLRIGRAVGLLNQKLQDRSFILMGPGRWGARDSRLGVPVG
                     YADINHSRMLIEIARHVGGFLPDVSFGSHFSLDLAESSIHYLALFPEEQGQSFDDRFL
                     LGSRNALAEVLPELADLADVVRLIDVRAASGGLSLSVDMDGDSQQAWGYLAG"
     gene            complement(2245..3885)
                     /locus_tag="VD788_15690"
     CDS             complement(2245..3885)
                     /locus_tag="VD788_15690"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HXV77756.1"
                     /translation="MATRRLETVVRGVDLLHDPLLNKGTAFTAAERDALGLRGLLPPG
                     VQTLDNQVSRVIENFRKQPGPLEKYTYLMALQDRNEALFYRVVVDHLEEMMPIIYTPT
                     VGQACQEYGHIFRRPRGIYLSLEDAGRVAEVLCNWPHREAAVIVVTDGERILGLGDLG
                     VNGMGIPVGKLTLYTACAGVHPARCLPVMLDVGTDRRSLIDDPLYLGLRRERARGEEY
                     DALVAEFVEAIQRVFPGALIQFEDFANRNAFRLLERYRDRVCCFNDDIQGTAAVVLAG
                     LLSALRVTGGRLEDQRLLFLGAGSASIGCAELIVSELGSLGLSPAQARQRCWLFDSKA
                     LVVAGRTDLTPQKRRFAHEHAAIGDFPAAVETLRPTAIIGASGQPGVFTERVVSTMAR
                     INDRPIVFSLSNPTSKSECTAEQAYRWSDGRAVFASGSPFDPVVYRGRTLVPGQGNNA
                     YIFPGIGLATVRCGIRRITDEMFSAAARTLAGLASPHEFERGCIFPPLASIRRVSTRI
                     AAAVAEVAYGAGLAAAAEPDDLVEAMEASVYQPGYRDS"
     gene            4052..4288
                     /locus_tag="VD788_15695"
     CDS             4052..4288
                     /locus_tag="VD788_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77757.1"
                     /translation="MSASLRCKITGCDPDPCGVCRRCGGERATTHDWRQAERERPCFA
                     RQVCTKCEREREQPDHDWQAKPNGLQCSRCGMTI"
     gene            4299..6248
                     /locus_tag="VD788_15700"
     CDS             4299..6248
                     /locus_tag="VD788_15700"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HXV77758.1"
                     /translation="MGGKRRATRARRLAAWWAGLATSCIVPFALAEPVPLEPGHWVVR
                     AEEHAFETHLGRPSLLLRNGVAWVDGSRFKDGVIEFDVAFTGERGFSGVAWRMQDERN
                     YEQFYLRPHQSGRPDACQYTPTYAGLTSWQLYHGPGYGAAVDYRTDGWNHVKLVVSGT
                     QAEVYVNGGPTPALFVVRQKREVESGRVGLNSYSAPAHFSNFSYRQDAQPELAGEPPP
                     AVGAPSGAILRWQVSDVFDGAVLGDATTLDPAIRAGRDWTSLDAEESGLANLGSVRAL
                     GPGQDTVFARASIDSEAARSTLLRFGYSDRARVYLNDALLYRGDNGYRSRDYRYLGTI
                     GWFDEVVLPLRPGENELWIAVSESFGGWGVQAAIDDAAGLSPSGAAPELPSTDLFLVP
                     IDGVVVGAPRRLTDRDGYDNQPAFLPDGERLVYSAADDGRTDVWQLDLVTGDRQRLRA
                     TAESEYSPTPIPGRPAVSVVRDYGESKQQLWAFPLDGGPPESLLPGVGPVGYHAWIDE
                     RSVLLFVLGDPLTLEIARVGAGASRVVGYSPGRALARIPSTGEMSYVDKRQATWWLVT
                     LDPSTGESRRLMPTLPETEDYAWAPDGAVWAGRASGLFRRSLTDDEWRMIADFAADGI
                     DGISRLVFSPDGRRLVLVARPVPRP"
     gene            complement(6273..7997)
                     /locus_tag="VD788_15705"
     CDS             complement(6273..7997)
                     /locus_tag="VD788_15705"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXV77759.1"
                     /translation="MPELGFLRDLVVVFGVALVVVTLLGRLRVPSIAGYIVAGILIGP
                     AALGLVGDPHKTEVLAEVGVVLLLFGIGLELSLERIRRLWRAVLIGGAIQVGTTVPCA
                     AAVAHWSGVGTASAVFLGFVVAVSSTAIVLRGLSARGELDAPHGRLAVGILIFQDLCV
                     VPMMLTVPILAGRGGSTAAALLGLGAGTIVLGTAVLATRVAAPWMLNAASKSRQRDVF
                     VLAVAVICLGVAWAASAVGVSVALGAFLAGLVVSGSEFRHQALADVIPLREVLASVFF
                     VSVGMLLDPRQLASHVGEIIVILAAILVGKFALLLVTGALMRLPLRVCVLTGVALCQV
                     GEFSFVLLRAGMEHGLVAEPRMGNLLAAVILSMLVTPFLLGFGPRLAAGVGRITPLTR
                     LLEVRSLEEEPPRRPLDGHVVIAGYGFAGRQLARSLRRSGHPYVVVDLNVENVRLAIR
                     DGVRAFFGDVTSPEVLDHLRIADARDLVLTVNDPDAARRAASLARGRAPGLRIIARAA
                     YLEDKPALLEAGANEVVAAEVEAAVEISYLILKSRGADGATLDDLLGRIRSEQTADPH
                     EREPRLAR"
     gene            8110..9156
                     /gene="ilvE"
                     /locus_tag="VD788_15710"
     CDS             8110..9156
                     /gene="ilvE"
                     /locus_tag="VD788_15710"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR01123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HXV77760.1"
                     /translation="MNEKPLPDWSRLGFALTETDWIYVAHATGRQSPSWDEGQFRPFA
                     PIEVSPAAAFLSYGQGVFEGLKARRVEDGRIVLFRPESNAARFRRSAVRLGLPAFPEE
                     RFVATVAELTRRNSRWVPPAGSGSFYLRPVLLASEPRLGLGLCERFTTVFYGSPVGPY
                     FSGGESRGLKLRVLEQGRVAPGGTGAAKAMGNYAGTLPLAAEWKQRGFDDVLFLDAKG
                     AGLVSETVGSNVFAVLDSGTLVTPPLDDQILPGVTRDSVLRIAREICGLNVEERPLPI
                     DELLDRAAEMFCTGTAYRLQPVGELSHRDRVKRFAATATRDALLRVLEGIQQGQRDDP
                     FDWVRTIGTEERRR"
     gene            9153..10133
                     /gene="gnd"
                     /locus_tag="VD788_15715"
     CDS             9153..10133
                     /gene="gnd"
                     /locus_tag="VD788_15715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HXV77761.1"
                     /translation="MRLAMIGLGRMGANMARRLMRGGHEVVAFDLDREAVDAFTSEGA
                     EAAYELSEAVAKLRPPRIAWVMVPAGAASESVVSRLAESMTAGDIVVDGGNGYFKDDI
                     RRHAKLAAAGVHYVDVGVSGGVWGLERGYSQMVGGDERAVATITPILRSLAPGEHAAS
                     PTPGREGKRTTADEGFLHCGPAGAGHFVKMVHNGIEYGLMQAYAEGFHLLDSAGQDDD
                     GRGYRYEIDAAEVAEVWRRGSVIGSWLLDLTSAALVEDPKLDHYSGRVADSGEGRWTV
                     MAAVEQAVPADVLSTSLYARFRSREQATFADKLLSAMRFKFGGHVERSRT"
     gene            10138..10911
                     /locus_tag="VD788_15720"
     CDS             10138..10911
                     /locus_tag="VD788_15720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXV77762.1"
                     /translation="MIDSHCHLTHRRFAADRHAVVARAAGAGVEHCVTIGTGVDDARG
                     ALELAGALPQRVSPTAGLDPFSAHAAGDGFDDALSVLAELLAGGGFVALGEIGLDYHY
                     DLDPRPLQRTRLERQLELAVRVDLPVVIHVRDAHRDMADVLAAHGSARGVIHSFTGGP
                     AEAERYLGLGWSLAFNGVLTFRNATGVREAAALVPDPALLVETDSPYLAPVPHRGKRC
                     EPAWVARTVETLAELRRTTVDHVDRVTTANARRLFRLGA"
     gene            10989..14309
                     /locus_tag="VD788_15725"
     CDS             10989..14309
                     /locus_tag="VD788_15725"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXV77763.1"
                     /translation="MIRTADIAPWLAALSVGLVAAAPLTAGPSGAGDADDEKWEVADP
                     PGERRSVKIDTDETTWSNLDVSPDGRSIVFDMLGDIYRVPIDGGAAAALTDGIEWNYQ
                     PRFSPDGSRLVFVSDRDGADNLWVMRADGSDARPITSEKEHLVHNPSWSPDGRYVVAK
                     KSFMSTRSIPAGEIWLFHHGGGDGLQLTERPHGKQDQKNMAEPAFSPDGRYVYYSRDA
                     TPGRVWEYNKDSTGQIFVVRRLELATGEDETFVGGPGGAVRPTPSPDGRYLAFVRRVP
                     GLDSALFVKDLDDGKEWPLHDRFERDLQESSGSEGNAPAFAWTPDSRSIVFWSAGKIR
                     RVDVASREASVIEIRVTADKQIQPALRVPVEVAPDRVAIRMPRWVTRSPVDETIVFQA
                     LGYLWVTRPGSEPRRVTTQNRDFEFWPSFSADGRFLVYTSWNDAELGRVRTVALRGGE
                     SRVLTPEPGHYVEPRVSADGRSVAYRKITGGYLLSPLGSLDPGLYVVPTDGGGDPVRV
                     SRAGFAPQFAADDDRLYFTDTVDENRAVLKSVDRTGHDERTHLAGDRVTEFRLSPDGR
                     WVAFVEGYDVYLAPHTPTGRTVDVSADAKSVPVRRISQRAGESLHWSAASDTLLWSHG
                     RTLYQRRLEDAFSFLEGAPRELPEPVTTGVEIVFDVPADKPRGKIALVGARVVTMRDA
                     SGGPEVIEDGTVLVEGNLIAAVGPRDEVGVPAEAFVVDLAGKTLIPGLIDVHAHGAMA
                     RDELTPQQNWIQFSNLAFGVTTIHDPSNDSSEIFAAAELQRSGAIVAPRIFSTGTILY
                     GAHLPGLTAAVGDAEDALFHVRRMKELGAISVKSYQQPRRDQRQQIVDAGRQLGVMVV
                     PEGGMKFQHNMSEIVDGHTGIEHSIPLRTCYDDVVQLWAQTEVGYTPTLGVAFGGLSG
                     ETYWYQHDDVWRNERLMRFSPRSVVEPRAMRREMAPDSHFNHVDVARFAKRLIEAGVS
                     VQIGAHGQREGLASHWEMWMLEQGGFTPWEALRSATIYGARYVGLDGDVGSIEAGKLA
                     DLVVIDGNPLENLRRSEYVSQTMINGRLYDTATMNQIAPDRVARAPFFFELEGGDTVP
                     EPTAAWLAQLQRRMGWRH"
     gene            14345..15712
                     /locus_tag="VD788_15730"
     CDS             14345..15712
                     /locus_tag="VD788_15730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HXV77764.1"
                     /translation="MEGAVQQITHGIDRIVWERDLRELTGWRWLALRAARLVYVVIRD
                     LLTGEINLRAMSLVYTTLLSIVPLLAVSFSVLKVFGVHNQVAPLLRDFLRPLGDEGVE
                     LADDIVAFVENVQVGVLGALGVVLLIYTAISLMHKIEVALNFVWHVDGVRSLGRRVSS
                     FISVVLVGPVLVVAALGITASLRSTTVVRSIERIEPFGSLLERLSQWLPYLLGALAFT
                     LVYALVPNTRVKLRAAATGGVVAGILWQSVGLFFATFIASSARYAAIYSSFAVMIVTL
                     IWLYANWLILLIGSQIAFYVQNPHYLTRKPLRLDLSNRMKETLSLAVIYWIGANHVNH
                     VAPHTVESLADRLELPTGPVEQVLALLEQGGWVEKSAADPPGYLPARDLATIRLDELL
                     GAVRTAGETPLLNVGRVSVPEPVRRLAAEAIGAAEHRLGERTVRDICTDSRPAGVADG
                     QPADG"
     gene            15781..16365
                     /locus_tag="VD788_15735"
     CDS             15781..16365
                     /locus_tag="VD788_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77765.1"
                     /translation="MRSSVLTAILLAVVVVPAAAGVRLILRDGRVLEGTDIRREGQAY
                     LLTLEDGGIVPIPDALVEGVALSDEGTDEDAPATVWPPTGLTDSRPQQLAGEAVSPSL
                     RAEQTRALGPPSRFQQGVIDPNWYPESEWDADAGDRSRNDFAPSQWSESIVDSDWVPE
                     NAFPEDRTQFAPSRFQGDIVDPTWVPQDGFSRKG"
BASE COUNT         2523 a         5577 c         5795 g         2526 t
ORIGIN      
        1 tggtcgaacg cgtccagcgc gcgctgctca gccggcttca ccgcggcggc atcgccacgg
       61 tcgaccggat ccacgccctg gctcggcacg acgccgccgg cgagtcagcg ggcgacgacc
      121 gcgaacggga acgtcggcgg atgcaacaga tcgtctggga cctggcgtcg cgggcctttc
      181 cgccgaatga gatcgaagac gtgctgaacc tcgcgctgaa gcgcgaggag gcacggacgc
      241 tcgaggagat cgccaacctc tccgcggtct cgttcgggct gatcgcggac aaggtccgcg
      301 cgttctgccg gctgcccaag gcgacctcgc cgttgcccga agcggaagcg caggcggtgc
      361 gcgtggcgct gatccgccac ttcatctccg atcagctcga gttcatcgcc gtcgcgaaac
      421 agcagctgac cgtccgtgac ttcggcgagc tggtcgatca cgtgatcggt cccgaggcgg
      481 gcatcggccg gatcggcggc aaggcggccg gcctgttcct cgccgagcgc gtgctgcgcc
      541 gcgcgcgcca gtccgatccg gcggcacccg acatcccgat ccgctgcccc gagtcgttct
      601 acttgcgctc tgacgtcatc gagcggtttc tcgatcaagc cggtctggtc gagctgcaga
      661 gccacaagta caagagcatc ggcgagatcc gcgacgagta cccgacgatc gtcgacacct
      721 tcaagagcgc cgagtttccc gccgaggtct ccgatcagct cggttcgatg ttgcaggcgg
      781 tcgggccgca tccgctgatc gtgcgctcgt cgagcctgct cgaggaccgc ttcggcacgg
      841 cgttcgcggg gaagtacagc agcgtgttcc tgaccaacca gggcccgccg gcggagcggc
      901 tggcccacct gctccgagcg atcgccgagg tctacgccag cacgctccac gcggatccga
      961 tcagctatcg tcgcaagcac aagctgttgg actacgacga gaacatggcc gtgctgattc
     1021 aaaaagttgt cggcacgagc taccgtcatt acttcctgcc ggcgtgggcc ggcgtcgcgt
     1081 tctcgcgcaa cccgcaccgg aggaacccgc ggatccgcgc cgaagacggt atggcgcgga
     1141 tcgccttcgg cttgggcacg cgcgccgtcg accgcgtctc gggtgattgt gtgaggatgg
     1201 tgccgctcgg catgccgacg ctgcgccccg aggtcacgcc cgaggagatc tgccgccaat
     1261 cgcagcgcga ggtcgacctg ctcaatctgc gcgatcatcg gttcgaaaca atcctggcgg
     1321 gggaactgct gcaggaaggc gaggagttcc ccggcctcga tcaggtcgtc tccgttctcg
     1381 atcggggctt cctcagccgg ccggtcggaa cccgcgtgct cgccgctccg tcggatcttg
     1441 tcgtgacctt cgatcgctgg acgcatgact cgccctaccc gaggctgctc ggctgggtgc
     1501 tccggacgct cgagcgggcc tacggctgtc cggtcgacgt cgagttcgcg cacgacgggc
     1561 gcgagttcca cctgctgcag tgccgacccc aggtgctgcg tcgcgccgaa caggtccggt
     1621 tgccgacgga cgtcgcggtc cagcgccgcg tcttcctcgc cgatcgcgag atcgagagtg
     1681 gcagcgtcca cggtgtcgag ttcatcgtgc tgatcgatcc tcgcgattac ggccggcagg
     1741 actcgagcga gcgccggctc cgtatcggtc gcgcggtcgg actgctgaac cagaagctgc
     1801 aggaccgctc gttcatcctg atggggccgg gccgctgggg tgcgcgcgac tctcgactgg
     1861 gggttccggt cggctacgcc gacatcaacc atagccggat gctgatcgag atcgcgcggc
     1921 acgtgggcgg gttcctcccg gatgtctcgt tcggcagcca cttctcgctc gacctcgccg
     1981 agtcctcgat ccactatctc gcgctctttc ccgaggagca gggtcagtcg ttcgacgatc
     2041 ggttcctgct cggctcgcgc aacgcgctgg cggaggtgct acccgagctg gccgatctcg
     2101 ccgacgtggt ccggctgatc gacgtccgcg ccgcgagcgg cggcctgtcg ctctcggtcg
     2161 acatggatgg cgattcgcag caggcgtggg gctacctcgc aggctgacct tctcggcccg
     2221 gtacggcgga gccgtcggct ccgctcagga gtcgcggtag cccggctggt agaccgacgc
     2281 ctccatcgcc tcgacgaggt cgtcgggctc cgcggcggcc gccaatcccg cgccgtaggc
     2341 gacctccgcg accgccgccg cgatccgcgt cgaaacgcgg cgaatcgaag ccagcggcgg
     2401 gaagatgcag cctcgttcga actcgtgcgg cgaggccagg ccggccagcg tcctggccgc
     2461 cgccgagaac atctcgtcgg tgatccggcg gatgccgcag cgaacggtgg ccagaccgat
     2521 gccggggaag atgtacgcgt tgtttccctg acccgggacg agcgtccgtc cgcggtagac
     2581 cacggggtcg aacgggctgc cgctggcgaa cacggcccgt ccgtcggacc agcggtaggc
     2641 ctgctcggcc gtgcactcgg acttcgaagt cgggttcgac agcgagaaga cgattggccg
     2701 gtcgttgatc cgggccatcg tcgagacgac ccgttcggtg aagacgccgg gttggcccga
     2761 ggcaccgatg atcgccgtcg gtctcagggt ctcgaccgcg gccgggaagt cgccgatcgc
     2821 tgcatgttcg tgcgcgaatc gtcgcttctg tggtgtcaga tcggtcctac cggcgacgac
     2881 cagcgccttc gagtcgaaca gccagcagcg ttgccgcgcc tgcgccggcg agagtccgag
     2941 cgagccgagc tccgacacga tcagctcggc gcagccgatc gacgccgagc cggccccgag
     3001 gaacagcagg cgctggtcct cgagccgtcc gccggtgacc cgcagcgccg acagcaggcc
     3061 ggccagcacg accgcggcgg ttccctggat gtcgtcgttg aagcagcaga cgcgatcccg
     3121 ataccgctcg agcaggcgga acgcgttgcg gttggcgaag tcctcgaact ggatcagcgc
     3181 accggggaag acgcgctgaa tcgcctcgac gaactcggcc accagggcgt cgtactcctc
     3241 accccgcgcg cgctcgcgac gcagaccgag gtagagcgga tcgtcgatca gcgaccgacg
     3301 gtcggtgccg acgtcgagca tcaccggcag gcaacgtgcc ggatggactc cggcgcacgc
     3361 cgtgtacagc gtcagttttc cgaccggtat ccccatgccg ttcacgccga ggtcgcccag
     3421 gccgagaatc cgctcgccgt cggtcacgac gatgaccgcc gcctcgcgat gcggccagtt
     3481 gcacagcacc tcggcgacgc gaccggcgtc ctcgagtgag aggtagatgc cgcgcgggcg
     3541 ccggaagatg tgcccgtact cctgacaggc ctggccgacg gtcggggtgt agatgatcgg
     3601 catcatctct tcgagatggt cgacgacgac gcggtagaac agcgcctcgt tgcggtcctg
     3661 gagcgccatc agataggtgt acttctcgag cggcccgggc tgtttgcgga agttctcgat
     3721 cactcgcgag acctggttgt cgagcgtctg cacgccgggg gggagcaacc cgcgcaaccc
     3781 cagcgcgtcg cgctcggcgg cggtgaacgc ggttcccttg ttcagcagcg ggtcgtgcaa
     3841 cagatcgacc cctcgaacaa cggtctcgag cctgcgtgtc gccatcggac atcctcccgt
     3901 gagccgcccc ggcgagacga tcgcctcatc ggaactctag caccgtgtcc gcggtcgtgg
     3961 tctagggctt gacgaccgtt tcgggcctcg atcgaccggt cgccggctcg gctatactgc
     4021 ccgggctcgg ccctgggccg gtggagcgtc gatgtcggcc tcgctgcgat gcaagatcac
     4081 cggatgcgac ccggatccgt gcggcgtctg ccgccgttgc ggcggcgagc gcgccacgac
     4141 tcacgactgg cggcaggccg agcgcgaacg accgtgcttc gcccgtcagg tctgtacgaa
     4201 gtgcgagcgg gaacgcgaac agcccgacca cgattggcaa gccaagccca acggcctgca
     4261 gtgttcgcgc tgcggcatga cgatctgagc gaccgggcgt gggcggcaag cgacgagcga
     4321 cgcgggcccg acggcttgcc gcgtggtggg ccgggttggc gacgtcgtgc atcgtaccgt
     4381 tcgccttggc cgagccggtt ccgctcgagc ccggccactg ggtcgtgcgg gcggaagagc
     4441 acgcgttcga gacccatctc ggccggccga gcctgttgct gcgcaacggg gtcgcgtggg
     4501 tcgacggctc gcgattcaaa gacggcgtga tcgagttcga cgtcgccttc accggtgagc
     4561 gagggttcag cggtgttgcc tggcgcatgc aggacgagcg caactacgag cagttctacc
     4621 tccgtccgca ccagtcgggg agaccggatg cctgccagta cacaccgacc tacgccgggc
     4681 tgacgagctg gcagctgtac cacgggccgg gatacggcgc ggccgtcgac taccgcacgg
     4741 acggctggaa tcacgtcaag ctcgtcgtct ccggcaccca ggccgaggtc tatgtcaacg
     4801 gaggcccgac tccggcgttg ttcgtcgtgc gacagaagcg cgaagtcgag tcgggccggg
     4861 tcgggctcaa ttcgtactct gcgcccgccc acttctccaa cttcagctac cggcaggacg
     4921 cgcagcccga gctcgcggga gagccaccac ccgcggtcgg cgcacccagc ggggcgatcc
     4981 tgcgctggca ggtctcggat gtcttcgacg gcgccgtgct gggcgacgcg acgacgctcg
     5041 accccgcgat ccgagcgggg cgggactgga cgtcgctcga cgccgaggag agcggtctgg
     5101 ccaacctggg gagcgtgcgt gcgctcggtc ccgggcaaga cacggtcttc gcccgcgctt
     5161 cgatcgactc ggaggcggcg cgctcgacgc tgctgcgctt cggctacagc gaccgcgcgc
     5221 gggtctacct gaacgacgcg ctgctgtacc gcggcgacaa cggctaccgc agccgcgact
     5281 accgctacct cggcacgatc ggctggttcg acgaggtcgt cctgccgctg cggccagggg
     5341 agaacgagct gtggatcgcg gtcagcgaga gcttcggcgg gtggggcgtg caggccgcga
     5401 tcgacgatgc cgccgggctc tcgccgagcg gggccgcgcc ggagttgccg tcgaccgatc
     5461 tgttcctggt tccgatcgac ggcgtcgtgg tgggcgcgcc gcgccggctg accgaccgcg
     5521 acggctacga caaccaaccg gcgttcctgc ccgacggcga gcgtctggtc tatagcgccg
     5581 cggacgacgg ccgcaccgac gtctggcagc tcgacctcgt gaccggcgat cgccagcggc
     5641 tgcgcgccac cgccgagagc gagtactcgc cgacgccgat cccgggccgg cccgcggtct
     5701 cggtcgtccg cgactacggc gaatcgaagc agcaattgtg ggcgttcccg ctggacggcg
     5761 gcccgccgga gtcgctgctg ccgggtgtcg gcccggtcgg ctaccacgcc tggatcgacg
     5821 agcggagcgt gctgctgttc gtgctcggcg atccgctgac gctcgagatc gcgcgcgtcg
     5881 gcgcgggtgc gagccgcgtc gtcggctatt cgccgggccg agccctggcg cgcattccgt
     5941 cgaccggcga gatgagctac gtcgacaagc gtcaggcgac ctggtggctc gtgaccctcg
     6001 acccgtcgac gggcgaaagc cgccgtctga tgccgacgct gcccgagacg gaggactacg
     6061 cgtgggcgcc ggacggggcg gtgtgggccg gccgggcgag cggtctgttc cgccgctcgc
     6121 tgacggatga cgaatggcgg atgatcgccg acttcgcggc tgacgggatc gacgggatca
     6181 gccgcctggt cttctccccc gatggacgcc gtctggtgct cgtcgcccgt cccgtccccc
     6241 gaccctgacg cccgcgcgtg tccgttgcgg acctaacgtg ccaaccgggg ctcccgttca
     6301 tgcggatcgg cggtctgctc ggagcggatc cgtcccaaca ggtcgtcgag cgtggccccg
     6361 tcggcaccgc gactcttgag aatcaggtag ctgatctcga cggcagcttc gacctcggcg
     6421 gcgacgacct cgttcgcgcc ggcttcgagc agcgccggct tgtcctcgag gtaggccgcg
     6481 cgcgcgatga tgcgcaggcc gggcgcgcgc ccgcgagcga ggctcgccgc ccgccgtgcg
     6541 gcatcggggt cgttcacggt cagcaccagg tcgcgcgcgt cggcgatacg caagtgatcc
     6601 agcacttccg ggctggtgac gtcgccgaag aaggcgcgca cgccgtcgcg gatcgccagt
     6661 ctcacgttct ccacgttcag atcgacgacg acgtacggat ggccgctcct tctcagcgag
     6721 cgagccagct ggcgcccggc gaagccgtag ccggcgatga cgacgtggcc gtcgagcggt
     6781 cggcggggcg gctcttcctc gagcgaacgc acctccaaca gacgggtcag cggagtgatg
     6841 cggccgacgc cggcggccag ccggggcccg aagccgagca ggaagggggt caccagcatc
     6901 gacagaatca ccgcggccag caggttgccc atccgcggtt cggcgaccag gccgtgttcc
     6961 atgcccgcgc gcagcaggac gaacgagaac tcgccgacct gacacagcgc gaccccggtc
     7021 aacacgcaca cgcggagcgg cagcctcatc agcgcgcccg tcacgagcag cagtgcgaac
     7081 ttgccgacca ggatcgcggc gaggatcacg atgatctccc cgacgtggct cgccagctgc
     7141 cgggggtcga gcaacatgcc gaccgaaacg aagaagacgc tggccagcac ctcgcgcagc
     7201 gggatgacgt cggccaacgc ctggtggcgg aactcgctac cggagacgac cagaccggcg
     7261 aggaacgccc ccagcgcgac cgaaacaccg acggccgagg cggcccaggc gacgccgaga
     7321 cagatcaccg cgaccgccag cacgaagacg tcacgctgcc gtgacttcga cgccgcgttc
     7381 aacatccacg gggccgcgac gcgggtggcc aggaccgccg taccgagcac gatggttccc
     7441 gcgccgagcc cgagcagcgc cgccgcggtc gagccgccgc gtccggctag gatcggcacg
     7501 gtcagcatca tcggcacgac gcacaggtcc tggaagatca ggatgccgac cgccagccgt
     7561 ccgtgaggcg cgtcgagctc gccgcgcgcc gacagaccgc gcaggacgat cgccgtgctc
     7621 gaaacggcga cgacgaaacc gagaaaaacg gccgacgccg tccccacgcc cgaccagtgc
     7681 gcgaccgccg cggcgcatgg caccgtcgtg ccgacctgaa tcgccccgcc gatcagcacc
     7741 gcgcgccaca gccgccggat ccgctcgagc gacagctcga gtccgatgcc gaacagcagc
     7801 agcacgactc cgacttcggc cagcacttcg gtcttgtgcg gatcgccgac cagacccagc
     7861 gcggcgggac cgatcaggat gccggcgacg atgtacccgg cgatcgaggg cacgcgcaga
     7921 cgccccagca gcgtgacgac gaccagcgcc acgccgaaga cgacgaccag gtccctgagg
     7981 aatccgagct cgggcatcgt gacgaacgct ccgggcgtca tcttaaacgg cgattccccg
     8041 gcgcgcgacc ggtcctcgcc gtatggtgta atcttccggc gagcttctcc actccggtgc
     8101 ggtgcgacga tgaacgaaaa gccactcccc gactggtcgc gtctgggctt cgccctcacc
     8161 gagaccgatt ggatctacgt cgcccacgcg acgggacggc agagcccgag ttgggacgag
     8221 ggccagttcc gcccgttcgc cccgatcgag gtctcaccgg cggccgcctt cctgtcctac
     8281 ggacagggtg tgttcgaggg actcaaggcg cgccgtgtcg aggacggacg gatcgtgctc
     8341 ttccggccgg agtcgaacgc ggctcgcttc cgccgttcgg cggtccggtt gggcctgccc
     8401 gcgttccccg aggagcggtt cgtcgcgacg gtcgccgagc tgacccgtcg caactcgcgc
     8461 tgggtaccac cggccggcag tgggtcgttc tacctgcgcc cggtactctt ggccagcgaa
     8521 ccgcggctcg gtctcggcct gtgcgagcgg ttcaccacgg tgttctacgg cagcccggtc
     8581 ggtccgtatt tcagcggcgg tgagagccgc gggttgaagt tgcgcgtgct cgagcagggc
     8641 cgggtcgcgc cgggaggcac gggggcggcg aaggcgatgg gcaactacgc gggaacgctg
     8701 ccgctggccg cggagtggaa gcagcgcggc ttcgacgacg tgctgtttct cgacgcgaag
     8761 ggggcggggc tcgtcagcga gaccgtcggc tcgaacgtct tcgcggtgct cgactcgggc
     8821 acgctcgtga caccgccgct cgacgaccag atcttgcccg gcgtgacgcg cgactcggtg
     8881 ctgcgcatcg cgcgcgagat ctgcgggctg aacgtcgaag agcgaccgct gccgatcgac
     8941 gagctgctcg accgagcggc cgagatgttc tgcaccggga cggcctaccg tcttcagccg
     9001 gtgggcgagc tgtcgcaccg cgaccgggtc aagcggttcg ccgcgacggc gacacgggac
     9061 gcgttgctgc gggtcctcga gggaatccag caggggcaac gggacgaccc gttcgactgg
     9121 gtgcggacga tcggaacgga ggaacgacga cgatgagact ggcgatgatc ggtctgggtc
     9181 ggatgggcgc caacatggcc cgccgcttga tgcggggggg gcacgaggtc gtcgccttcg
     9241 acctcgatcg ggaagcggtc gacgcgttca cgtccgaggg cgccgaggcg gcctacgagt
     9301 tgtccgaggc ggtggccaag ctgaggccgc cgcggatcgc gtgggtgatg gtgcccgccg
     9361 gcgccgccag cgagagcgtc gtctcgcgac tggccgagtc gatgaccgcg ggagacatcg
     9421 tcgtcgacgg cggcaacggc tacttcaagg acgacataag acgtcacgcg aagttggccg
     9481 ccgccggcgt gcactacgtc gacgtcggcg tcagcggagg ggtgtggggc ctcgaacgcg
     9541 gttactcgca gatggtgggc ggtgatgagc gcgcggtggc gacgatcacg ccaatcctgc
     9601 gcagcttggc tccgggcgag cacgccgcgt cgccgacacc ggggcgcgag ggcaagcgca
     9661 cgaccgcgga cgaggggttt ctgcactgtg ggccggccgg cgccggtcac ttcgtcaaga
     9721 tggtgcacaa cggtatcgag tacgggctga tgcaggccta cgccgagggg tttcacctgc
     9781 tcgactcggc cggccaggac gacgacgggc gtggttaccg ctacgagatc gacgcggcgg
     9841 aagtggccga ggtgtggcgc cgcggcagcg tgatcggctc ctggctgctc gatctgacct
     9901 ccgcggcgct ggtcgaggat ccgaagctcg accactacag cggccgcgtc gccgattcgg
     9961 gcgaaggacg ctggaccgtg atggccgcgg tcgagcaggc ggtgccggcc gacgtgctgt
    10021 cgacctcgct ctacgcgcga ttccgctcgc gcgagcaggc gacattcgcc gacaagctgc
    10081 tgtcggcgat gcggttcaag ttcggcggtc acgtcgaacg gtcccgaacg tgacacgttg
    10141 atcgactcgc actgccacct gacccatcgg cggttcgcgg ccgatcggca cgcggtcgtc
    10201 gctcgtgcgg cgggtgccgg tgtcgagcac tgcgtcacga tcggcacggg cgtcgacgat
    10261 gcgcgcggcg cgctcgagct ggcgggagcg ttgccgcagc gcgtctcgcc gaccgcgggt
    10321 ctcgacccgt tttcggccca tgccgccggc gacgggttcg acgacgcgct gtccgtcttg
    10381 gccgagctgc tggccggcgg aggcttcgtc gcgctcggcg agatcgggct cgattaccac
    10441 tacgacctcg acccgcgacc gctgcaacgg acgcggctcg agcgccagct cgagctcgcc
    10501 gtccgcgtcg acctgcccgt cgtgatccac gtgcgcgacg cgcaccgtga catggccgac
    10561 gtgctggcgg cgcacggatc ggcgcgcggg gtgatccaca gcttcaccgg cgggccggcc
    10621 gaggccgagc gctaccttgg tctcggatgg tcgctggcgt tcaacggcgt gctgaccttc
    10681 cgcaacgcga ccggggtacg cgaagcggcc gcgctcgtcc ccgaccccgc gctgctcgtc
    10741 gagaccgaca gcccttacct cgcgccggtt ccgcatcgcg gcaagcgttg cgagccggcc
    10801 tgggtcgcac gcaccgtcga gacgttggcc gagctgcgcc ggacgacggt cgaccacgtc
    10861 gaccgcgtga cgacggccaa cgcccgccgc ctgttccgcc tgggtgcgta accgacggag
    10921 ccgccggggt ttcggctaga atcgacgagc cgcggcgacg gacgccgccc gtccggaggg
    10981 agtcgccgat gatccgaacc gcagatatcg caccgtggtt ggcggcgctc tccgtcgggt
    11041 tggtcgccgc cgccccgctg accgccggac cgtcgggcgc gggcgatgcg gacgacgaaa
    11101 agtgggaggt cgctgaccca ccgggcgaac gacgaagcgt gaagatcgac accgatgaga
    11161 cgacttggtc caacctcgac gtcagcccgg acggccgttc gatcgtgttc gacatgttgg
    11221 gcgacatcta tcgcgtgccg atcgacggcg gcgcggccgc agcgttgacc gacgggatcg
    11281 agtggaacta ccagccgcgg ttcagtccgg acggcagccg cttggtcttc gtcagcgatc
    11341 gggacggtgc ggacaacctg tgggtgatgc gggcggacgg ctcggatgcg cgtccgatca
    11401 cgagcgagaa ggagcacctg gtccacaatc cgtcgtggag cccggacggc cgctacgtcg
    11461 tggccaagaa gagcttcatg tcgacccgca gtattccggc cggcgagatt tggctgttcc
    11521 atcacggtgg tggcgatggc ctgcagctga ccgagcggcc ccacggcaag caggaccaga
    11581 aaaacatggc cgagcccgcc ttctccccgg acggccgcta cgtctattac agccgcgacg
    11641 cgaccccggg tcgcgtctgg gagtacaaca aggactcgac cggacagatc ttcgtcgttc
    11701 ggcggctcga gctggcgacg ggagaggacg agacgttcgt cggcggcccg ggcggggccg
    11761 tgcgaccgac gccgtcgccc gatggtcgtt acctggcctt cgtccgccgc gtcccggggt
    11821 tggacagcgc gctgttcgtc aaggatctcg acgacggcaa ggagtggccg ctccacgacc
    11881 gtttcgagcg cgacctgcag gaatcgagcg gcagcgaggg caacgctccg gcattcgcct
    11941 ggactcccga ttcgcgttcg atcgtctttt ggagcgccgg caagatccgc cgggtcgacg
    12001 tcgcgagccg cgaggcgagc gtgatcgaga tccgcgtcac cgccgacaag cagatccagc
    12061 ccgcactacg tgtcccggtc gaggtcgcgc cggaccgcgt ggcgattcgc atgccgcgct
    12121 gggtgacgcg gtctccggtc gacgagacga tcgtgttcca ggcgttgggc tacctgtggg
    12181 tcacgcgacc ggggtccgag ccgcgccgcg tcaccacgca gaatcgcgac ttcgagttct
    12241 ggcccagctt ttccgcggac ggtcgattcc tcgtctatac cagctggaac gacgctgaac
    12301 tcggccgggt acgcacggtc gcgctccgcg ggggggaaag tcgcgtgttg acgcccgagc
    12361 ccggacacta cgtcgagccg cgcgtctcgg ccgacggccg gtcggtcgcc taccgcaaga
    12421 tcaccggcgg ctacttgctc tccccgctgg gctcgctcga ccccgggctg tacgtcgtac
    12481 cgaccgacgg cggcggagat ccggtccgtg tgtcgcgcgc gggttttgcg ccgcagttcg
    12541 cggccgacga cgaccggctg tacttcaccg acacggtcga cgagaaccgc gccgtgctca
    12601 agagcgtcga tcgaaccggc cacgacgagc ggacccatct ggcaggtgac cgcgtcaccg
    12661 agttccgcct ctcccccgac ggccgctggg tcgcgttcgt cgaaggctat gacgtctatc
    12721 tcgctccgca cacccccacc gggcgaaccg tcgacgtgtc agccgacgcc aagtcggtgc
    12781 cggtccgccg catctcgcaa cgggccggcg agtcgctgca ttggtccgcg gcgagcgaca
    12841 cgctgttgtg gtcccacggc cgaacgctgt accagcgccg attggaagac gccttctcgt
    12901 ttctcgaagg cgcgccgcgc gagctgcccg aaccggtgac gaccggcgtc gagatcgtct
    12961 tcgacgtgcc ggcggacaag ccgcgcggca agatcgcgct ggtcggcgcg cgggtggtca
    13021 cgatgcgcga cgcgagcggc ggcccggagg tgatcgagga cggcacggtg ctggtcgagg
    13081 ggaatctcat cgccgcggtc ggaccgcggg acgaggtcgg cgttccggcc gaggccttcg
    13141 tcgtcgacct cgccggcaaa acgttgatcc ccgggttgat cgacgtccac gcgcatggcg
    13201 cgatggcccg cgacgagctg acgccccagc agaactggat ccagttctcg aacttggcgt
    13261 tcggcgtcac cacgatccac gacccgtcga acgacagctc ggagatcttc gcggccgcgg
    13321 agctgcagcg cagcggagcg atcgtcgctc cccggatctt ctcgaccgga acgatcctgt
    13381 acggcgccca ccttcccggt ctgacggccg cggtcggcga cgccgaggac gcgctgttcc
    13441 acgtgcgccg gatgaaggag ctgggcgcga tctcggtcaa gagctatcag cagccgcgac
    13501 gcgaccagcg ccagcagatc gtcgatgccg gccgtcagct cggtgtgatg gtggtgcccg
    13561 agggcgggat gaagttccag cacaacatga gcgagatcgt cgacgggcat accggcatcg
    13621 agcactcgat cccactgcgc acgtgctacg acgacgtcgt gcagctgtgg gcgcagacgg
    13681 aggtcggcta cacgccgacg ctcggtgtcg cattcggcgg gctgagcggt gagacctatt
    13741 ggtaccagca cgacgacgtc tggcgcaacg agcggctgat gcgcttcagc ccgcgctccg
    13801 tcgtcgagcc gcgagcgatg cggcgcgaga tggctcccga ctcgcacttc aatcacgtcg
    13861 acgtggcgcg tttcgccaag cggctgatcg aggccggtgt atcggtgcag atcggcgccc
    13921 acggacaacg cgagggcctg gcgtcgcatt gggagatgtg gatgctggag cagggcggat
    13981 tcacgccgtg ggaggcactg cgcagtgcga cgatctacgg agcgcgttac gtcggcctcg
    14041 acggcgacgt cggctcgatc gaagccggga agctggccga tctcgtcgtc atcgacggca
    14101 atccgctcga gaatctgcgt cgctcggagt acgtcagcca gacgatgatc aacggtcggc
    14161 tgtacgacac ggccacgatg aaccagatcg cgccggatcg cgtcgcacgc gcgccgttct
    14221 tcttcgagct cgaggggggg gataccgttc cggaaccgac cgcggcctgg ctcgcgcagt
    14281 tgcagcgacg gatgggctgg cgacactgac cgcgagcgtg gggcgtcgcg acgcgagggg
    14341 ttcgatggaa ggcgccgtgc agcagatcac gcacgggatc gaccggatcg tctgggagcg
    14401 cgatctccgc gagttgacgg gttggcgctg gctcgcgctg cgagctgccc ggctggtcta
    14461 cgtcgtgatc cgcgatctgt tgaccggcga gatcaacctg cgggcgatga gtctcgtcta
    14521 caccactctg ctgtcgatcg tcccgttact cgcggtcagc ttctccgtgc tcaaggtgtt
    14581 cggcgtgcac aaccaggtgg ccccgttgct gcgcgatttc ctgcggccgc tcggcgacga
    14641 gggcgtcgag ttggcggacg acatcgtggc gttcgtcgag aacgtgcagg tcggcgtgct
    14701 cggcgcgctg ggcgtcgtgt tgttgatcta caccgcgatc tcgctgatgc acaagatcga
    14761 ggtcgcgttg aacttcgttt ggcacgtcga cggggtgcgg agcttgggcc ggcgcgtaag
    14821 cagcttcatc tcggtcgtgt tggtcgggcc ggtgctggtc gtcgccgcgc tgggcatcac
    14881 ggcgtcgctt cgaagcacga ccgtcgtgcg gagcatcgag cgcatcgagc cgttcggcag
    14941 cctgctcgag cggctgtcgc agtggttgcc ctacctgctg ggggcgctgg cgttcaccct
    15001 ggtctacgcc ctcgtgccga atacccgggt caagctgcgt gcggcggcga cgggcggggt
    15061 ggtcgccggc atcctctggc aatcggtcgg gctgttcttc gcgaccttca tcgcctcgtc
    15121 ggccagatat gcggcgatct actcgtcgtt cgcggtgatg atcgtgacgc tgatctggtt
    15181 gtacgcaaac tggctgattc tgctgatcgg ctcgcagatc gcgttctacg tgcagaaccc
    15241 gcactacctg acgagaaagc cgctccgact cgatctgagc aaccggatga aggaaacgct
    15301 gtcgctcgcg gtgatctact ggatcggtgc gaaccacgtg aatcacgtcg cgccgcacac
    15361 cgtcgagtcg ctggccgacc ggctcgagct gccgacgggg ccggtcgagc aggtgctggc
    15421 attgctcgag cagggcggct gggtcgaaaa gagtgccgcg gacccgccgg gctaccttcc
    15481 ggcccgcgac ttggcgacga tccgcctcga cgagctgctg ggcgcggtgc gcaccgccgg
    15541 cgagactccg ttgctcaacg tcggtcgggt atcggtaccg gagcccgtcc gccggctcgc
    15601 cgccgaggcg atcggcgccg ccgagcaccg tctcggcgag cggaccgtac gggacatctg
    15661 caccgactca cgccccgcgg gggtcgccga cgggcagccg gccgacggct gacgcgagaa
    15721 aacccgtgct gaccgctccg gccgcccggc cgcggaccgg ttcggaggta acatcagggt
    15781 atgcgatcga gcgtcctcac cgcgatcctg ctggccgtcg tcgtcgtacc ggcagccgcc
    15841 ggggtccggc tgatcctccg cgacggacgc gtgctcgagg gaaccgacat acgccgcgag
    15901 ggccaggcct acctgctcac gctcgaagac ggcggtatcg tgccgatccc cgacgcgctg
    15961 gtcgagggcg tcgccctctc ggacgaggga accgacgagg atgcgcccgc gacggtttgg
    16021 ccgccgacgg ggttgaccga cagccggccg caacagctcg cgggcgaggc ggtcagtccg
    16081 tcgctacgag cggagcagac tcgcgcgctc gggcccccgt cgcgcttcca gcagggcgtg
    16141 atcgatccga actggtaccc ggagagtgaa tgggatgccg atgcgggcga tcgatcgcgc
    16201 aacgacttcg caccgtccca gtggtccgag tcgatcgtcg attcggactg ggtgcccgag
    16261 aacgccttcc ccgaggatcg gacccagttc gcgccgtcac ggtttcaggg cgacatcgtc
    16321 gacccgacct gggtcccgca ggacggattc tcccggaagg gctagcacct gcacggcgag
    16381 gatcgcgttc agcgggccgg ggcgaccaag ccgatcgcac c
//
LOCUS       DASPGN010000541         3831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1742144, whole genome shotgun sequence.
ACCESSION   DASPGN010000541 DASPGN010000000
VERSION     DASPGN010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1742144"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1415)
                     /locus_tag="VD788_15740"
     CDS             complement(<1..1415)
                     /locus_tag="VD788_15740"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXV77766.1"
                     /translation="MLRGAGVRPVASYEPDPLVRSATLDSRRVEPGDLFFALRGLEVD
                     GVDFVPQAAERGASAIVAGVPRPSWLDPAIAWVEVDEARRAAALLSRELQGRPDLALT
                     LVGITGTNGKTTVSYLIESIARAAGMRAGRIGTVGHAFGDVERGAIRTTPEAPEFYRL
                     LAEMRDASTELVAIEVSSHALALDRVAGARFQVAAFLNLGRDHLDFHQTAAAYFEAKA
                     RLFDALDESQWAVVPAGDPRGEQIRRRTTAHVLGFGREAGADVRLRDEHCGVDGSRAV
                     LDTPRGSVPIRTFLLGRFNLDNIAAAAACALSAGIGPDAIAAGALALERVPGRVERID
                     RGQPFAVVVDYAHTADALQNVLRFATDVTAGRVHVVFGCGGERDRGKRAEMGRVAAAS
                     GATIYITSDNPRGEDPERIIDEIEQGVLAVPGGAGRCTRLADRRAAIEAAILAAAADD
                     AVVIAGKGHETTQTVAGRVVT"
     gene            complement(1442..3220)
                     /locus_tag="VD788_15745"
     CDS             complement(1442..3220)
                     /locus_tag="VD788_15745"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXV77767.1"
                     /translation="MPTTTRNRLILVSSMVVLVALAIVGRLYQLQVIRCEGLSRRAAE
                     QHSSKLVVPAMRGTIVDRNGKPLALSTETRSLYAHPQRVGDPERAAELLGPLLDRPRA
                     AILRDLRSDKSFVFLARFLEPDVADRIAALGLPMNGAEPFGFEHEPRRVYPRKELAVH
                     VVGFATIDGDGVEGIERSFDADLKGDPRIYLLMHDAHRRGPRELIGEPRKKPSDVVLT
                     IDVVLQHVVERELARAMRETRARAGSVILLDAPSGEVLALANRPTTDANEFGQATHAA
                     RRNRALVDQYEPGSTFKVVPLAAAIELNRVGARDRIYCEQGVYRRRGRTIRDLSRNGT
                     LTPAEILSKSSNIGMAKIAKKMTPEEMSRVINRFGFGAKTGVELPGEEQGLFEDPSRW
                     SGYSQESISFGQEIGVTAMQMAAAISVFANDGVMVPPRIVLGKRGPDGRLRPMPPTAT
                     RRVVSERTASTVARMLEGVVDHGTGRRAAVAGYRVAGKSGTAQRAVDGVYSTSAVTAS
                     FGGFAPADDPRLVLLVVLDTPRIEDNGGGQAAAPVFARIMAESLSYLRVPGDDPERQA
                     TLSLEHDQPALPARHAAAAAMGKDAG"
     gene            complement(3256..3666)
                     /locus_tag="VD788_15750"
     CDS             complement(3256..3666)
                     /locus_tag="VD788_15750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77768.1"
                     /translation="MTHDQKTSTAHGWRNRPLVRETDARRLFSLWKTLLVAVVALAPG
                     AAYVVQQSQCLKLSYQVADLEVTHEQLVKQELELLAQAETLEELDDIERWALGRRGLD
                     RPAAEDVVIVRIDRSRPSALLARRSSDTTDDIPR"
BASE COUNT          553 a         1410 c         1297 g          571 t
ORIGIN      
        1 aacgtgacca cacggcccgc gaccgtctgg gtcgtctcgt gccccttgcc cgcgatcacc
       61 accgcgtcgt ccgccgcggc ggcgagaatc gcggcctcga tcgccgctcg gcggtcggcc
      121 aggcgcgtgc aacgccccgc cccaccgggc accgcgagca caccctgctc gatctcgtcg
      181 atgatccgct ccgggtcttc gccacgcggg ttgtccgacg tgatgtagat cgtcgccccg
      241 ctcgcggccg cgacacgacc catctcggcg cgcttgcctc ggtcgcgttc gccgccacag
      301 ccgaagacga cgtgtacgcg gccggcggtc acgtcggtcg cgaagcgcaa cacgttctgc
      361 agcgcgtcgg ccgtgtgcgc gtagtcgacg acgaccgcga acggctggcc gcggtcgatc
      421 cgctcgacgc ggccgggcac ccgctcgagc gccagcgcgc ccgcggcgat cgcgtcgggc
      481 ccgatgccgg cggacaacgc gcaggccgcc gctgcggcga tgttgtccag attgaaccgt
      541 ccgagcagga aggtgcggat cggcacgctc ccgcgaggcg tgtcgagcac cgcgcgagac
      601 ccgtcgacgc cgcagtgctc gtcgcgcagc cggacgtcgg cgccggcttc gcgaccgaag
      661 ccgagcacgt gcgccgtggt ccgccggcgg atctgctcgc cgcgggggtc gcccgccggc
      721 acgaccgccc attgcgattc gtcgagcgcg tcgaacagcc gcgccttcgc ttcgaagtaa
      781 gccgccgcgg tctggtggaa atcgaggtgg tcgcggccga gattgaggaa cgccgcgacc
      841 tggaaccgcg cacccgcgac gcggtccaac gccaacgcat gggacgagac ctcgatcgcg
      901 accagttcgg tcgacgcgtc gcgcatctcg gccagcaatc gatagaactc aggtgcttcg
      961 ggcgtggtgc ggatcgcccc gcgctccacg tcgccgaagg cgtgaccgac ggtgccgata
     1021 cgcccggcgc gcattccggc ggcacgggcg atcgactcga tcagatacga cacggtcgtc
     1081 ttgccgttgg tgccggtgat cccgaccagc gtcagcgcca aatccggccg cccttggagc
     1141 tcgcgcgaca gcagcgcggc cgcgcgccgc gcctcgtcga cctcgaccca ggcgatcgcc
     1201 gggtcgagcc acgacggccg aggcacaccg gcgacgatcg cgctcgcgcc ccgttcggcc
     1261 gcttgcggca cgaaatcgac gccgtcgacc tcgagcccgc gcagcgcgaa gaacagatct
     1321 cccggctcga cccgccgcga gtcgagcgtg gcgctccgca ccagcggatc cggctcgtac
     1381 gacgcgaccg gacggacgcc cgcgccgcgc aacaacgacg acaatctcgt gcccggaccg
     1441 ctcaacccgc gtctttcccc atcgccgccg cggcagcgtg tcgcgccggc agcgccggct
     1501 ggtcgtgctc gagcgacagc gtcgcttgcc gctcgggatc gtcaccgggc acccgcaagt
     1561 agctcaacga ctcggccatg atccgtgcga agaccggcgc ggccgcctgc ccgccgccgt
     1621 tgtcctcgat gcgcggcgtg tcgaggacga cgagcagcac caaccgcggg tcatcggccg
     1681 gcgcgaagcc gccgaacgag gccgtcaccg cactcgtcga gtaaactccg tccaccgctc
     1741 gctgtgcggt gcccgacttg ccggcgacgc gatagcccgc gaccgccgcg cgccggccgg
     1801 tgccgtggtc gaccacgccc tcgagcatcc gtgcgacggt gcttgccgtt ctctcgctca
     1861 ccacgcgtcg cgtcgcggtc ggcggcatcg gccgcagcct gccgtcgggt ccgcgcttgc
     1921 ccagcacgat ccgcggcggc accatcaccc cgtcgttggc gaagaccgag atcgcggcgg
     1981 ccatctgcat cgcggtcacg ccgatctcct ggccgaacga gatcgactcc tgcgagtaac
     2041 ccgaccagcg cgacggatcc tcgaacagcc cctgctcctc gccgggcagc tcgacgccgg
     2101 tcttggcgcc gaagccgaag cggttgatca cacggctcat ctcttccggc gtcatcttct
     2161 tcgcgatctt ggccatgccg atgttgctcg acttgctgag gatctccgcc ggcgtcagcg
     2221 tcccgttgcg ggacagatcg cggatggtgc ggccgcgtcg gcggtagact ccctgttcgc
     2281 agtagatgcg atcgcgtgcg ccgacccgat tcagctcgat cgccgccgcg agcggaacca
     2341 ccttgaaagt cgaacccggt tcgtactggt cgaccagcgc gcggttgcgc cgagccgcgt
     2401 gcgtggcctg cccgaattcg ttggcgtccg tcgtcggccg gttggccagc gccaacacct
     2461 cgccgctcgg tgcgtcgagc aggatgaccg accccgcccg cgcgcgcgtc tcgcgcattg
     2521 cccgcgccag ctcgcgctcg acgacgtgct gcagcaccac gtcgatcgtc agcacgacgt
     2581 ccgacggttt cttgcgcggc tcgccgatca gctcgcgcgg tccgcgccga tgggcgtcgt
     2641 gcatcagcag gtagatcctg gggtcgccct tgagatcggc atcgaaactg cgctcgatgc
     2701 cctcgacgcc gtcgccgtcg atcgtggcga agccgacgac gtgtaccgcg agctccttgc
     2761 gcggataaac gcgtcgtggc tcgtgctcga agccgaacgg ctcggcgccg ttcatcggca
     2821 gaccgagggc cgcgatgcgg tcggcgacgt ccggctcgag gaaccgtgcg aggaagacga
     2881 acgacttgtc ggagcgcagg tcgcgcagga tcgcggcccg cggtctgtcg agcagcgggc
     2941 caagcaactc tgcggcgcgc tcgggatcgc cgacgcgctg cgggtgggcg tacaacgacc
     3001 gcgtctccgt gctgagcgcg agcggcttgc cgttgcggtc gacgatcgtc ccgcgcatcg
     3061 ccggcaccac cagcttgctg ctatgctgtt cggcggcccg ccggctcagt ccctcgcagc
     3121 ggatgacctg cagctgatac agccgcccga cgatggcgag cgcgacgagc acgaccatcg
     3181 agctgacgag gatcaggcga ttgcgggttg tcgttggcat gacggtccgt tagccgggtc
     3241 cccgccgcgt ccggctcagc gggggatgtc atcggttgtg tcgctcgacc ggcgggccag
     3301 cagagcggac ggtcgcgagc gatcgatgcg gacgatgacg acgtcctcgg cagcgggccg
     3361 gtccaacccg cgtctaccga gagcccaccg ttcgatgtcg tccagttctt cgagcgtctc
     3421 ggcctgggcc agcagctcga gctcctgctt caccagttgc tcgtgggtca cctcgagatc
     3481 cgcgacctga taggacagct tcaagcactg gctttgctgg accacgtatg ccgcgcccgg
     3541 ggcgagcgcc accacggcga cgagcagggt cttccacagc gaaaacaggc gtcgcgcgtc
     3601 ggtctcgcgg accaacggcc ggttgcgcca tccatgcgcc gtggaggttt tctggtcgtg
     3661 tgtcacagtc gttctccgac gcgcagccgt gcgctgcgcg aacgtgggtt gcgcccgatc
     3721 tcctcgtccg acggccgcac cggcctggcg gtcaacacgc ggatccggtt ctcccgaccg
     3781 cagccgcaca ccggaaggtg cggagggcag atgcagcgct cggcgagagc c
//
LOCUS       DASPGN010000542         6147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1155303, whole genome shotgun sequence.
ACCESSION   DASPGN010000542 DASPGN010000000
VERSION     DASPGN010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155303"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VD788_15755"
     CDS             <1..286
                     /locus_tag="VD788_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77769.1"
                     /translation="RPVTDGAGIAPVEIAQPTLAPGVPTSSAGDTVARERYDDAIDEI
                     EHLRLRLQEAEASLKKSESRARKLADQTDTYRARIDRTAARLERLIERLG"
     gene            333..409
                     /locus_tag="VD788_15760"
     tRNA            333..409
                     /locus_tag="VD788_15760"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:367..369,aa:Pro,seq:ggg)
     gene            489..1247
                     /gene="surE"
                     /locus_tag="VD788_15765"
     CDS             489..1247
                     /gene="surE"
                     /locus_tag="VD788_15765"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXV77770.1"
                     /translation="MGQSFVLVTNDDGFDAPGLAALVEAVSPLGRVVVVAPDREQSGS
                     SHALTLVRPLRAYRVADDRHRVDGTPTDCVHLAINSLTGGKMPDLVVSGMNRGLNVGD
                     DVTYSGTVAGAMEGTLLHIPSIAFSVGIESDGQADFTRAVPFARRISEIVLERGLQPG
                     VLLNVNFPAASYRGVRITRQGTRTYRATALERLDPAGRPYYWIDSVSATPTDEPDGDH
                     RAIREGYVSVSPLTSNLTHAGSLERLREWNLGLA"
     gene            1249..1902
                     /locus_tag="VD788_15770"
     CDS             1249..1902
                     /locus_tag="VD788_15770"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXV77771.1"
                     /translation="MVHTESLRRERLVRELVEKGIRDPRVLEAMGAVPRERFVQEALR
                     GEAYSGAALPIGAKQTISQPFIVARMCELAAPAGGGKALEIGTGSGYHAAVLSHLFEL
                     VYSIERLRELAALALKTLRGLGIHNVHTKIFDGSYGWSEFAPFRAILVTAAAPDVPKP
                     LLEQLEDGGRLILPIGDGEADGEQVLTVVTRNGTKWKRTTHGPCRFVPLRGKYGWPV"
     gene            1899..2528
                     /locus_tag="VD788_15775"
     CDS             1899..2528
                     /locus_tag="VD788_15775"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HXV77772.1"
                     /translation="MSGEPRPRGGLHYRLYDWVLHWSAHRHAQSALFVLSFAEASFFP
                     VPPDVLLMSMTLARPVRAVRYALIATIGSVTGGLAGYAIGYGFWTALEPYAYRYLHFM
                     KFTPEVFAAVQHKYQANAFLALFMAGFTPIPYKIFTVAAGVFGVAIPVFIVASLIGRA
                     GRFLLVALLLRFAGPTIKPFLDRYLGWLTIAFVALLILGFWVVGLLGNA"
     gene            2602..2678
                     /locus_tag="VD788_15780"
     tRNA            2602..2678
                     /locus_tag="VD788_15780"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2636..2638,aa:Pro,seq:cgg)
     gene            2772..3968
                     /locus_tag="VD788_15785"
     CDS             2772..3968
                     /locus_tag="VD788_15785"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HXV77773.1"
                     /translation="MDSLPSRHWRSVLSDVERAATRSGRSGVGCFVIEGTRLHERALR
                     ASAPPREVLVADSYGRTDDLRASRLLAALEACGCDVFRVPDTVIAACTAGREIGAIIG
                     LVPLPREASLAAVLRAAPERPAVALVAVDVEDPGNVGALIRTALAGGADLFAAVGTTE
                     PFHPKAVRTSMGSLFRLPLATYPTFDAFSDALREIGGCSYGATTRGGEALPRTVFGPE
                     PVALVIGSEAFGLDPTAEAHVDRRVTIPMRPRVDSYSVNAAAAVLLYEVRRPPRRALR
                     RRLAVPYDDRYGTRSGQKDTTMDAREGAKKATDHASERWLVSGRVQGVGFRYHVLRAA
                     RNLGVRGDVRNLPDGRVELRVRAPRELLSDLLRAVREGPPAARVESVERVSLPGEARF
                     DGFSIR"
     gene            3980..4504
                     /locus_tag="VD788_15790"
     CDS             3980..4504
                     /locus_tag="VD788_15790"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HXV77774.1"
                     /translation="MLEPELKRTIREIPDFPKPGINFYDVSTLFRNGRAFRATVDQMA
                     ARYRDRSIDAFAGIEARGFMLAGAMSYALGVGTVLVRKPGKLPHETDAENYTLEYGEA
                     TIEVHRDAIETGQRIVVVDDLLATGGTAAAAGRIIGRLGGRVLGYAFMVELGFLSGRK
                     TLGAADVFSLLRYD"
     gene            4514..4591
                     /locus_tag="VD788_15795"
     tRNA            4514..4591
                     /locus_tag="VD788_15795"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4549..4551,aa:Arg,seq:cct)
     gene            4714..5394
                     /locus_tag="VD788_15800"
     CDS             4714..5394
                     /locus_tag="VD788_15800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77775.1"
                     /translation="MARISRKELKRDEFIEATKEAENWLEENWRLVLRIAGGVVVVGL
                     LVGGWFWYGSLSRRQAAGLLQDGLAAYQTAQEAGFVDATALDTALGKFERAARKGGGS
                     PVGLVAEYYRGVALHRLGRSEESIDSLGEVAVSAGAPASLRGSASAVLAEVYAATGEQ
                     DRAVAMLESLIAAEPPTYPIDQALLALAELHERSGDTDRARQLWQRIVDEYPARGAAD
                     EARQRLGS"
     gene            complement(5391..>6147)
                     /locus_tag="VD788_15805"
     CDS             complement(5391..>6147)
                     /locus_tag="VD788_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77776.1"
                     /translation="APSNRKRRRPSRRRGTKTTTFPSDRLFALLLCAALTLPIGAQQA
                     AEVFPLAPADDSVVGPREPFRVGYRGIDDSALREARFRIDLHPVAREANTLRYDQRRR
                     KRGWSPGEPGVMLYRPGRPIADGSYEWDVSVWDGVMWRSSGERFRLRIDSVPPSPVEN
                     LTVTYDAERGVLRLDWNPVVTDVDGGPEYVARYHVYRHSRADRAPKAEPFEIAEVVDP
                     TVELPVSGPDDARLWFFRVSAEDLAGNETGRPR"
BASE COUNT          909 a         2051 c         2172 g         1015 t
ORIGIN      
        1 ccggccggtg accgacggcg cgggcatcgc gccggtcgag atcgcccaac cgaccctcgc
       61 ccccggcgtt cccacgtctt ccgccgggga cacggtggcg cgcgagcgtt acgacgacgc
      121 gatcgacgag atcgagcatc tgcgtttgcg gctgcaggaa gcggaagcga gcctcaagaa
      181 gtcggaaagc cgggcccgca agctcgcgga ccagaccgat acctatcgcg ccaggatcga
      241 ccgcacggcg gcgcggctcg agcggttgat cgagcggctg ggctgagtcg cggatcgacg
      301 gcagcggatg tgctaatctc tgcgcgtacg gtcggggcgt ggcgcagtct ggtagcgcac
      361 ttgcttgggg tgtaagtggt cgttggttca aatccaatcg ccccgaccac tttgttccca
      421 cccgcgcgcg acgggatccg caccgagccg aatacgcggt cgcgtgcgga ggtgcggggt
      481 cagacgagat gggccaatcg ttcgttctcg tgaccaacga cgacggtttc gacgctcccg
      541 gcctcgcggc actggtcgag gcggtctcac cgctcggtcg cgtcgtcgtc gtcgcgcccg
      601 accgtgaaca gagcgggtcg agccacgcgc tgaccctggt ccgaccgttg cgcgcctatc
      661 gcgtcgccga cgatcgccac cgcgtcgacg ggacgccgac cgactgcgtg cacctggcga
      721 tcaacagtct gacgggcggc aagatgccgg atctggtcgt ctcgggcatg aaccgcgggt
      781 tgaacgtcgg agacgacgtc acctattcgg gaacggtggc cggcgcgatg gaggggacct
      841 tgctccacat accgtcgatt gccttctccg tcggcatcga gtcggacggc caggccgatt
      901 tcacccgagc cgtgccgttc gctcgtcgga tctccgagat cgtgctcgag cgcgggctgc
      961 aacccggcgt gctgctcaac gtgaatttcc ccgccgcgag ctaccgcggt gtgcggatca
     1021 cgcgacaggg cacacggacc tatcgcgcca ccgcgctcga gcggctcgat ccggccggcc
     1081 gcccgtacta ctggatcgac agcgtgagcg ccacaccgac cgacgagccg gatggcgacc
     1141 accgggcgat tcgtgagggc tacgtctcgg tcagcccgct gacgtcgaac ctgacccacg
     1201 ccggctcgct cgagcgtttg cgcgagtgga atctggggct cgcctgaggt ggtgcacacc
     1261 gaatcgttgc ggcgcgagcg cctggtgcgg gagctggtcg agaaggggat acgcgatccg
     1321 cgcgtgctcg aggcgatggg cgcggtgccg cgcgagcgat tcgtccagga agcactgcgc
     1381 ggtgaggcgt acagcggcgc ggcgctgccg atcggtgcca agcagacgat ctcccaacct
     1441 ttcatcgtgg cgcggatgtg cgagctggcg gcccctgccg gcggcggcaa ggcgctcgag
     1501 atcggcaccg gctcgggtta tcacgccgcg gtcctctcgc acctattcga gttggtctac
     1561 tcgatcgagc gactacgcga gttggcggcg ctcgcgctga agacgctgcg aggtctcggc
     1621 atccacaacg tccataccaa gatcttcgac ggcagctacg gctggagcga gttcgcgccg
     1681 ttccgcgcga tcctagtcac cgcggccgcg cccgacgtgc cgaaaccgct gctcgagcag
     1741 ctcgaggacg ggggccgctt gattctgccg atcggcgacg gcgaggccga cggcgagcag
     1801 gtgctgaccg tcgtgacccg gaacggtaca aagtggaagc gaaccacgca cggtccgtgt
     1861 cgattcgtgc ccctccgcgg aaagtacgga tggccggtgt gagcggcgag ccacggccga
     1921 gaggcgggct gcattaccgg ctctacgact gggtgttgca ctggtcggct caccggcacg
     1981 cgcaatcggc gctgttcgtc ctgtcgttcg cggaagcctc gttcttcccg gttccgcccg
     2041 acgtgctgtt gatgtcgatg acgctggcac gaccggttcg cgcggtgcgt tacgcgctga
     2101 tcgcgaccat cggttccgtg acgggaggtc tggccggtta cgcgatcggc tacggattct
     2161 ggaccgcact cgagccctac gcctatcgct acctgcactt catgaagttc acccccgagg
     2221 tcttcgccgc ggtccagcac aagtaccaag cgaacgcgtt cctcgcgttg ttcatggctg
     2281 gcttcacgcc gatcccgtac aagatcttca ccgtcgcagc cggtgtgttc ggggtcgcga
     2341 tcccggtctt catcgtggcc tcgctaatcg gccgcgccgg ccgtttcctg ctggtggcgc
     2401 tgttgctacg cttcgcgggg ccgacgatca agccgttcct cgaccggtac ctgggctggc
     2461 tgacgatcgc cttcgtcgcg ctgctgattc tcgggttctg ggtggtggga ctcctcggca
     2521 acgcatagtg gcaggcgaac cgctcgccga gcgttgcgcg gcgggcggac ggttgttacc
     2581 atggccgcgc ttctacgagg tcggggcgta gcgcagtctg gtagcgcacc tgcttcgggt
     2641 gcagggggtc gagtgttcaa atcactccgc cccgaccaag tcgatccacc cgcggtgaag
     2701 cgcgacggtg acccggcggt cgccggtcgc ggcggacgtg ctgcgggagc gacgtaccgg
     2761 gaagacccgc gatggattcg ctgccctcgc gccactggcg cagcgtgctg tctgacgtcg
     2821 agcgggcggc gacccgttcg ggtcgcagcg gcgtcggctg tttcgtgatc gagggaaccc
     2881 gcctgcatga gcgggcgttg cgtgcttcgg ccccaccgcg ggaggtgttg gtggccgaca
     2941 gctacggccg caccgacgac ctccgcgcct cgaggttgct cgcggcgctc gaggcgtgcg
     3001 gttgcgacgt gtttcgcgtg cccgataccg tgatcgcggc ctgcacggcg gggcgggaga
     3061 tcggggcgat catcggactg gtgccgctac cccgcgaggc gtcgctggcc gcggtgctgc
     3121 gggcggcgcc cgagagaccg gccgtcgcgt tggtcgcggt cgacgtcgaa gatccgggga
     3181 acgtcggcgc cttgatccgt acggcgctcg ccggcggggc cgacctgttc gccgcagtcg
     3241 gtacgaccga gcccttccac cccaaggcgg tccgcacctc gatgggctcg ctgttccggc
     3301 tgccgctcgc gacctacccg acgttcgacg cgttcagcga cgccctgcgc gagatcggcg
     3361 gatgttcgta cggcgcgacg acccgaggcg gcgaggcgtt gccgcgcacc gtcttcggcc
     3421 cggagccggt cgcgttggtc atcggcagcg aggcattcgg cctcgacccg acggccgaag
     3481 cccacgtcga ccggcgtgtg acgatcccga tgcgcccgcg cgtcgactcg tactccgtga
     3541 acgccgcggc cgccgtcctg ctctacgagg tccggcgccc gccgcgccga gccttgcgcc
     3601 ggcggctcgc ggtaccttac gatgatcgct acgggacgcg gtcggggcaa aaggacacga
     3661 cgatggatgc gcgcgaaggc gcgaagaaag caaccgatca cgccagcgag cgctggttgg
     3721 tcagtgggcg cgtccagggg gtcggattcc gttaccacgt gttgcgggcg gcgcgcaatc
     3781 tcggcgtgcg gggtgacgtt cgcaatctgc cggacgggcg ggtcgagctc cgcgtccgcg
     3841 cacctcgcga gctgttgtcg gatctgctcc gggcggtccg cgagggacct cctgccgcgc
     3901 gcgtcgagtc ggtcgagcgg gtgtcgctcc cgggcgaggc gcgcttcgac ggcttttcga
     3961 tccgctgaga ggaagcgcga tgttggaacc cgagctgaag cggacgatcc gcgagatccc
     4021 cgactttccc aagccgggca tcaatttcta cgacgtttcg accctgtttc gcaacggccg
     4081 cgcgtttcgc gcgacggtcg accagatggc ggcgcggtac cgcgatcgtt cgatcgacgc
     4141 attcgccgga atcgaagcgc ggggcttcat gctcgccggc gcgatgtcct acgccctcgg
     4201 cgtcggaacg gtgctcgtgc gcaaaccggg caagctgccg cacgagacgg atgcggagaa
     4261 ttacacgctc gagtacggcg aggccacgat cgaggtccac cgcgacgcga tcgaaaccgg
     4321 tcagcgcatc gtcgtggtcg acgatctgct ggccaccggt gggacggccg ctgcggcggg
     4381 tcgtatcatc ggccgactcg gtggccgcgt cctgggctac gcgttcatgg tcgagctcgg
     4441 ctttctctcc ggcagaaaga ccctgggtgc ggccgatgtc ttttcgctgc tacgctatga
     4501 ctgaggtggg tgcgggcctg tagctcagct tggatagagc gcgagattcc taatcttgag
     4561 gtcgcaggtt caagtcctgc caggctcacc atcctcaatg cggggccgcg cgtgcggtcg
     4621 aacagcggcg attggcctcc gccggagcga ttcggtacca tgtggcccgg ccagccggac
     4681 ctggcgcggg gcgacccgcg tggagtgcga cagatggcga ggatttcccg aaaagagctc
     4741 aagcgagacg agttcatcga ggccacgaaa gaggccgaaa actggctcga ggaaaactgg
     4801 cgcctcgtgc tccggatcgc gggcggcgtg gtggtcgtcg gtctgctcgt cggcggctgg
     4861 ttctggtatg gcagcctgag tcgacggcag gccgcggggc tgctgcagga cggactggca
     4921 gcctatcaga cggcccagga ggcgggcttc gtcgatgcga cggcactcga cacggcgctc
     4981 ggcaagttcg agagggcggc ccgcaagggg ggaggctcac ccgtcgggtt ggtggccgag
     5041 tactaccgtg gcgtcgcgtt acatcgcttg gggaggagcg aggaatcgat cgattcgctg
     5101 ggcgaggtcg cggtcagcgc cggcgcgccc gcgtcgcttc gcggttcggc gtccgcggtg
     5161 ctggcggagg tctacgccgc caccggcgag caggatcggg ccgtcgcgat gctcgagtcg
     5221 ctgatcgcgg ccgaacctcc cacctacccg atcgatcaag cgctgctcgc gctggccgag
     5281 ctgcacgagc ggtcgggcga caccgatcgc gcccggcagc tctggcagcg gatcgtcgac
     5341 gagtacccgg cccgcggcgc ggccgacgag gcgcgccagc ggctggggag ctaacgcggc
     5401 cgtcccgttt cgttccccgc caggtcctcg gcgctgactc gaaagaacca cagtcgtgcg
     5461 tcgtccggcc ccgacacggg aagctccacc gtcggatcga cgacctcggc gatctcgaac
     5521 ggctcggcct tgggcgcccg gtcggcgcgc gagtggcgat agacgtgata gcgcgcgacg
     5581 tactcgggcc ccccgtccac gtccgtgacg accgggttcc agtcgagtcg cagcacgccc
     5641 cgttccgcgt cgtaggtcac cgtgagattc tcgacgggcg acggtggaac ggagtcgatc
     5701 cgcaggcgaa accgctcgcc gctcgagcgc cacatcaccc cgtcccacac cgatacatcc
     5761 cattcgtacg acccgtcggc gatcggacgc ccggggcgat agagcatgac tcccggctca
     5821 ccgggactcc aaccccgctt ccgccgtcgc tggtcgtagc gcagcgtgtt cgcctcccgc
     5881 gcgacggggt gcagatcgat acgaaagcgc gcttcacgca gcgcgctgtc gtcgatcccc
     5941 cgataaccga cacgaaacgg ctctcgcggg ccgacgacgc tgtcgtccgc cggcgccaac
     6001 ggaaagacct cggccgcctg ctgcgctccg atcggcaggg tcagcgcggc gcagagcagc
     6061 agcgcgaaca gccgatcaga agggaatgtc gtcgtcttcg taccccggcg tcggctcggc
     6121 cgtcgccgtt tccggttgct cggcgcc
//
LOCUS       DASPGN010000543         3328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_804883, whole genome shotgun sequence.
ACCESSION   DASPGN010000543 DASPGN010000000
VERSION     DASPGN010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804883"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /gene="ggt"
                     /locus_tag="VD788_15810"
     CDS             <1..1391
                     /gene="ggt"
                     /locus_tag="VD788_15810"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXV77777.1"
                     /translation="AHRDMYLDPDDNVIEGASLYGHLAVGVPGTVAGLWEVHERFGTL
                     AWSDLVAPAVKLAAEGFVVPEAMVKSVLEMEERVAGRSNFDDYFGPMRVGERFRQPEL
                     AETLQRIADQGPDDFYRGRTAELIVAEMARGGGLITREDLAAYEIRWREPLRSPWRNF
                     EILSAPLPSSGGFALIQLLKIKDQLGRHFAGLPHNSPQYVHLVAEIEKRVFADRAEYL
                     GDPDFVETRIHELLSEDYLERRALEVDPTHISADVGPGTAIEAHDTTHYSIVDSRGNA
                     VSNTYTLNISYGSGVVVEGAGFLLNDEMDDFSVKPGVPNIYGVVGSHANAIEPGKRML
                     SSMTPTILLEDGEVAIVVGTSGGSTIFTSVFQTLVNVLEFGMTPADAVGATRFHHQLL
                     PPDLIVYSPGRPLPAETVAALGGKGYRVEPNSWEFGDMQIIVRDGTRLLPASDPRHRG
                     ESRVVESVEPNR"
     gene            complement(1408..1971)
                     /locus_tag="VD788_15815"
     CDS             complement(1408..1971)
                     /locus_tag="VD788_15815"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXV77778.1"
                     /translation="MRVRGFVLVFAMLALLTGGCAKKHSRTTMSPEAAPPPAPVSAPL
                     EPSVAPPPPPAVRDPLDGDLTTVNRYLREQGLLDDLLFDYNRHELTPDARRQLEAVAR
                     FLKERPQFQVALEGHADERGTVEYNVALGQKRAVSARSHLDTMGVSSDRLRASSYGEE
                     RPLCKEPSESCWKRNRRVHFEVVGRVE"
     gene            complement(2180..>3328)
                     /locus_tag="VD788_15820"
     CDS             complement(2180..>3328)
                     /locus_tag="VD788_15820"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXV77779.1"
                     /translation="RLTARLVLSHRTGLPNWGGERLDFETDPGAAFGYSGEGFVLLQR
                     ALEQTTGETLDALTRREVFEPLGMVRSRFSWPEGTELGRVTRHDPVGRPQPGNEPRES
                     NAAASLHTTASEYARFVVAWLNGELLGEPAADEALRPAVRIERDSVDEEETATARLAW
                     GLGWGIELPAAGAPGHPIYWHWGDNGPSKAFVAFDLATGSGLVYFANSANGLAIGPAL
                     VADVVGEMGATFEWLGYERYDSPGYAERLAGAVAESEGRYGEATAAFRSALAADPEDE
                     ETARRVEWLTDLLDRQQRPVALPRSLLERYAGSYGPRVLGVEEGMLHYQRGEGTRYRL
                     VPLSETLFALDGLVTFRIEVALDDRGEPVKIIGHYVSGETDESARDPA"
BASE COUNT          535 a         1153 c         1125 g          515 t
ORIGIN      
        1 ccgcgcaccg cgacatgtat ctcgatccgg acgacaacgt gatcgagggc gcgagcctct
       61 acggtcatct cgccgtcggc gtacccggga ccgtcgcggg cttgtgggag gtgcacgagc
      121 ggttcggcac gctggcctgg agcgatctgg tggcgcccgc ggtgaagctg gcagccgagg
      181 gtttcgtcgt gcccgaagcg atggtcaaga gcgtgctgga gatggaggag agggtcgccg
      241 gtcgcagcaa cttcgacgac tacttcggcc cgatgcgggt cggcgaacga ttccgccagc
      301 cggagctcgc cgagacgctt cagcgcatcg ccgaccaggg accggatgac ttctatcgcg
      361 ggcgcaccgc ggagctcatc gtcgccgaaa tggcgcgcgg cggcggcttg atcacgcgcg
      421 aggatctcgc ggcgtacgag atccggtggc gcgagccgct gcgctctccc tggcgcaact
      481 tcgagatcct gtcggcgccg ttaccgagct caggcggctt tgcgttgatt cagctgctga
      541 agatcaagga ccagctcggt cgtcatttcg cgggcctacc gcacaactcg ccgcagtacg
      601 ttcatctcgt cgccgagatc gagaaacggg tgttcgcgga ccgcgcggag tacctcggcg
      661 atcccgattt cgtcgagacg cgcatccacg agctgctgag cgaggactac ctcgagcgcc
      721 gcgcgctgga ggtcgatccg acgcacattt ccgccgacgt cggtcccggt accgcgatcg
      781 aggcgcacga cacgacgcat tactcgatcg tcgacagccg gggcaacgcc gtgtcgaaca
      841 cctacacgct caacatcagt tacggcagtg gtgtcgtcgt cgagggcgcc ggcttcctgc
      901 tcaacgacga gatggacgat ttcagcgtca agccgggcgt gccgaacatc tacggcgtcg
      961 tcggcagcca cgccaacgcc atcgagcccg gcaagcgcat gctgtcgtcg atgacaccga
     1021 cgatcctgct cgaagacggc gaggtcgcca tcgtggtcgg cacgtcgggg ggctcgacca
     1081 tcttcacctc ggtgttccag accctcgtca acgtcctgga gttcggcatg actccggcgg
     1141 atgcggtggg cgccacgcgc ttccatcacc agctgctgcc tccggacctg atcgtctaca
     1201 gtcccggccg accgctgccg gcggagaccg tcgccgcgct cggcggcaaa ggctaccggg
     1261 tggaaccgaa cagctgggag ttcggcgaca tgcagatcat cgtccgcgac ggcacgaggc
     1321 tcctgccggc atccgatccg cgtcaccgtg gcgagtcgcg cgtcgtcgag tccgtcgagc
     1381 cgaacaggta acgcgacgct cgacggctca ctcgactcgt cccacgacct cgaagtggac
     1441 ccgccggttc cgcttccagc agctctcgct cggctccttg cacagcggac gctcctcgcc
     1501 gtagctcgac gcacgcagcc ggtcgctgga aacgcccatc gtgtcgaggt gcgaacgagc
     1561 cgacacggca cgcttctgcc ccaacgcgac gttgtactcg acggtgcccc gctcgtccgc
     1621 atgtccttcg agcgcgacct ggaactgcgg gcgttctttc aagaaccggg cgaccgcttc
     1681 gagctgccgg cgggcatcgg gggtcagctc gtggcggttg tagtcgaaca gcagatcatc
     1741 gagcagaccc tgctcccgca ggtaacgatt gaccgtggtc aggtcgccgt ccagcggatc
     1801 tcgcaccgcc ggcggcggcg gtggcgccac gctcggctcg agcggggcgg ataccggcgc
     1861 aggcggaggc gccgcttcgg gactcatcgt cgtgcgggag tgcttcttcg cgcaacctcc
     1921 cgtgagcaag gcgagcatcg cgaacaccag gacgaaacca cgcacgcgca tgggtactct
     1981 cctcctcgat caagtgcgcg atctcgtggc aatcggtgtg ccaagctgcc gcgtgtcggt
     2041 gggagtggga aaagcgctcg tgcgatccgg gtcgcgatcc agcggtcgta atcggtctcg
     2101 ccgtgaaaag acggaatcgt gagcgggcgg cccgatccga gacgcaggtt cccgagcctg
     2161 gaacgcggcg tcgggccggt cacgccggat cgcgagccga ctcgtcggtc tcaccgctca
     2221 cgtagtgacc gatgatcttg accggctccc cgcggtcgtc gagagccacc tcgatgcgga
     2281 aggtgacgag cccgtcgagg gcgaacaggg tttcggacaa cggtaccagc cggtaccgcg
     2341 tcccctcgcc gcgctggtag tgaagcatgc cctcctcgac gccgaggacc cgcgggccgt
     2401 agctcccggc gtagcgctcg agcagcgatc gcggcagcgc gaccgggcgc tgctgccgat
     2461 cgagcagatc ggtaagccat tcgacgcgcc gcgccgtctc ctcgtcctcg ggatcggccg
     2521 cgagcgccga tcggaaggcg gcggtggcct cgccgtagcg accctcgctc tcggccaccg
     2581 cgcccgccag gcgctcggcg taaccggggg aatcgtaccg ctcgtagccg agccattcga
     2641 aggtggcgcc catctcgccg accacgtcgg ccaccaacgc cggtccgatc gcgagcccgt
     2701 tggcgctgtt ggcgaagtag acgagaccgg agccggtggc gaggtcgaac gcgacgaagg
     2761 ccttcgacgg accgttgtca ccccagtgcc aatagatggg atgcccgggc gcaccggcgg
     2821 ccggcagctc gatcccccag ccgagacccc aggcgagccg cgcggtagcc gtttcctcct
     2881 cgtccacgct gtctcgttcg atccggaccg ccgggcgcag tgcctcgtcc gcggccggtt
     2941 caccgagcag ttcgccgttc agccaggcga cgacgaaacg cgcgtactcc gacgccgtcg
     3001 tgtgcaagct ggcggccgcg ttcgactcgc gcggctcgtt gccgggctgc ggccgaccga
     3061 ccgggtcgtg acgcgtcacc cgcccgagct cggtgccctc cggccacgag aaacggctcc
     3121 gcaccatgcc gagcggctcg aagacctcgc gccgggtaag cgcgtcgagg gtctctcccg
     3181 tcgtctgctc gagggcccgc tgcaacagga cgaagccctc gcccgaatag ccgaaggccg
     3241 cgccgggatc ggtctcgaag tcgagccgct cgccacccca gttgggcagg ccggtccggt
     3301 gcgagagaac caggcgggcg gtcaaccg
//
LOCUS       DASPGN010000544         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_921900, whole genome shotgun sequence.
ACCESSION   DASPGN010000544 DASPGN010000000
VERSION     DASPGN010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921900"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VD788_15825"
     CDS             complement(<1..726)
                     /locus_tag="VD788_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77780.1"
                     /translation="MALGKNTARSILMVVGLFAAVWAIYRFFDDLTQSGSELYQSLLP
                     MLSLALVVLALALAGVLIRNLVRLVVEHKRGILGARLRSKLVFYYFALVLLPALVLFY
                     GSAHVIKQSVEAVLKTPLEDLTRRSGAIVQEWRDYLQSQALDIARSIASEIEERGYGQ
                     ADDVESGELLRRWRLDDDLRHIRVTREGRVLAEAHADTLEVDAGRREELKRLVDSLEL
                     DVSESGREQAHIDYLGDGLLAHAA"
     gene            complement(726..1307)
                     /locus_tag="VD788_15830"
     CDS             complement(726..1307)
                     /locus_tag="VD788_15830"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HXV77781.1"
                     /translation="MITRIGLACLLLAVVLSPVRTAREAKPAITDLAAVRDGNRVRVS
                     YRLVDCLEPETLERIASGIPIRFKHKVELVERRPGLFQGDRVHSRTLLETAVQYDSLT
                     ERYQLSRLTEPKTRRVQADAEPVVERLSSDSMEEMRAWLTVVDDAPLLAPDDAADVDY
                     HLRVEVAIGRKWFLLVFPSSHTIHSESPTAFGD"
     gene            complement(1304..2194)
                     /gene="lpxC"
                     /locus_tag="VD788_15835"
     CDS             complement(1304..2194)
                     /gene="lpxC"
                     /locus_tag="VD788_15835"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HXV77782.1"
                     /translation="MYNRQTLARRRTIQGVGLHSGRPVSMTLSPAAAGSGVVFVRTDA
                     GGVEIPADLDHAGPSFYATVIRQGDVVVRTIEHLMAALYALLVDDVRVELDGDEVPIL
                     DGSSRPFVDAILDAGRRELDTPREYITLVRPIVVAEDEKRISAYPCREYRVTYAIEFD
                     HPHLQHQELSASLWGGTAFVDKLAPARTFTFEREVEALRARGLALGGSLENAVVVGDH
                     GILNPDLRFADEFVRHKMLDLTGDLSLLARPLRAHVVAFRAGHDLHAKLARRIRDARD
                     AWYLARWNEESPNLEAAAEA"
     gene            2549..3694
                     /locus_tag="VD788_15840"
     CDS             2549..3694
                     /locus_tag="VD788_15840"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77783.1"
                     /translation="MSITPQPTAPDGSGDERLADLFLYLEEGVSRFRSGDLRGAIAAW
                     EEALSANPEHQGAWLDLARARSSVGEYEGAERCARRALELHPECAEAWFELGNTQTSL
                     GELDEAVGSYRTALELAPDLIEAWNNLGNTYARQERFEEAIRCYREAVERRPTTYQTW
                     FNLGNAHGVLSQMRQAIWAYENAASHSPDNNDVWYNLAYARACCGLLEAAVDGYRRAA
                     QLTPDDYEAWFNLGHAYADLGRDEQAADAYRKALAVDQTDHEAWNNLGNVLLRLQRPD
                     EAIDCYRRALEITAGYHPSWNNLGNALEEQGELNEAAEAYARAIELAPRRPTYLLNRA
                     SVLARIGRAEDAVDDLVHALDTAPEIRGLLPGFKEFGSLLRHPRLRR"
     gene            3815..>4539
                     /locus_tag="VD788_15845"
     CDS             3815..>4539
                     /locus_tag="VD788_15845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77784.1"
                     /translation="MELLEDQVHGLAVAESPAEVFKALIDGARIAAPRTAVFLVRQDQ
                     LKGWGTVGYPVEAATRQRAFVGPSDRGWFGEVLRVEQPVPGVDGRDEYPDFGQPSTDQ
                     FVGLAVRVKGRPIAVLVGERTTGQLPWHPQVLGILVRVAQLRLDLDLVRRKLAAAGTS
                     PGPPLSPGGPAAPGRGATRKPSVIPPAITTAPVEPTPESVTEVAAATPAPVEDGPRGD
                     ARHEAARRYARLVATDIRLYNEEA"
BASE COUNT          760 a         1549 c         1541 g          689 t
ORIGIN      
        1 ggccgcgtgt gccagcagcc cgtctcccaa gtagtcgatg tgggcctgct cgcgtcccga
       61 ctcgctcacg tcgagctcga gcgagtcgac cagccgtttc agctcctcgc ggcgcccggc
      121 gtcgacctcg agtgtgtcgg catgcgcctc ggccagcacc cgtccctcgc gggttacgcg
      181 gatgtgccgc aggtcgtcgt cgaggcgcca gcgacgcaac aactcgcccg actcgacgtc
      241 gtccgcctgt ccatagccgc gttcctcgat ctcgctcgcg atcgaccgcg cgatgtcgag
      301 agcctgcgac tgcagatagt cccgccactc ctgcacgatc gcaccggatc gccgggtcag
      361 gtcctcgagg ggcgtcttga gcaccgcttc gaccgactgc ttgatgacgt gggccgaacc
      421 gtagaacagc actagcgcgg gcagcagcac cagcgcgaag tagtagaaaa cgagcttgct
      481 acgcagccgt gcgccgagga tcccacgctt gtgttcgacg accagccgca ccaggttgcg
      541 aatcagcacg cccgccagcg ccagcgccag gacgaccagc gccagcgaca gcatcggcaa
      601 cagcgattgg tacagttccg agccggactg cgtcaggtcg tcgaagaagc ggtagatcgc
      661 ccacaccgcg gcgaacaacc cgaccaccat caggatcgat cgcgcggtgt tcttaccgag
      721 ggccatcagt caccgaacgc cgtcgggctc tccgagtgga tcgtatggct gctcggaaag
      781 acgagcagga accactttcg accgatcgcc acctcgaccc gcaagtgata atcgacatcc
      841 gcggcgtcgt ccggcgccag cagcggcgcg tcgtcgacga cggtcagcca cgcccgcatc
      901 tcctccatcg agtccgagct cagccgctcg acgaccggtt cggcgtcggc ttgaacacgg
      961 cgggtcttcg gctcggtgag gcgagacaac tggtagcgct cggtcaacga gtcgtactgc
     1021 accgccgtct cgagcaacgt tcgcgagtgg acgcgatcgc cctggaacaa tcccggccgt
     1081 ctttcgacga gctcgacctt gtgcttgaac cggatcggga tgcccgacgc gatccgctcg
     1141 agcgtctccg gttccaggca atcgacgagg cggtacgaca cccggactcg attcccgtcg
     1201 cgaacggctg ccagatcggt gattgcgggc ttggcctcgc gcgcggtgcg cacgggcgac
     1261 agcacgacgg ccagcagtag acacgctaac ccgattcgcg tgatcacgct tcggccgccg
     1321 cctccaggtt cggcgactcc tcgttccagc gggccaggta ccacgcgtct cgcgcatcac
     1381 gaatccgccg cgcgagcttg gcgtgcaggt cgtggccggc acggaaggcg acgacgtggg
     1441 cgcgaagcgg acgcgccaac agcgacaggt cgcccgtcag gtcgagcatc ttgtgtctga
     1501 cgaattcgtc ggcgaagcgc agatccgggt tcaggattcc gtggtcgccg acgacgacgg
     1561 cgttttccaa cgacccgccg agcgccaagc cgcgcgcgcg cagcgcttcg acctcccgtt
     1621 cgaaggtgaa ggtgcgcgcc ggggcaagct tgtcgacgaa cgccgttcca ccccacaggc
     1681 tggccgaaag ctcctgatgc tgcaagtggg ggtggtcgaa ttcgatggcg tacgttacgc
     1741 ggtactcacg acacgggtag gcggagatcc gcttctcgtc ctccgcgacg acgatcggac
     1801 gcaccaacgt gatgtactct cgcggggtgt cgagctcgcg ccgtccggcg tcgaggatcg
     1861 cgtcgacgaa cgggcgggag gagccgtcga ggatcggcac ctcgtcgccg tcgagctcga
     1921 cgcggacatc gtcgacgagc aacgcgtaga gcgcggccat caggtgctcg atcgtacgta
     1981 cgacgacgtc gccttgtcgg atgaccgtgg cgtagaagct cgggccggcg tgatcgagat
     2041 ccgcgggaat ctcgacaccc ccggcgtcgg tccggacgaa gacgaccccc gagcccgccg
     2101 ctgcgggcga cagggtcatc gagaccggcc gcccggagtg caggccgacg ccttggatgg
     2161 tgcggcgccg cgccagggtc tgccggttgt acatcggact ccctccgtcg ccgccggtgc
     2221 ggacccgtcc attctataga atccgcgacg gaaagccccg cgccgaagcc cgttacagaa
     2281 agtacctccg ctccttggtg gtccgcgacc caacatccga caggaatttc gattgaccct
     2341 gcgaatcgcg gcgtgctaga tatctggcag acgccgatcc tgctgccgga tcggcgggat
     2401 aggccgtata tgaacgtcgg cgaacctcgc ggttcacgtg tgttgggggg acaacggtga
     2461 ccggatctga ggcacgagcc gagtgacgcg cagcgagagt cagcgcgtcg gcgggggcga
     2521 gcgttcgctc cgaggcgaac ctctgccgtt gtcgatcacg ccgcaaccga cggcgcccga
     2581 cgggtcgggc gacgagcggt tggccgacct gttcctctac ctcgaggagg gcgtctctcg
     2641 tttccggtcc ggggacctgc gcggggcgat cgcggcgtgg gaagaggcgc tcagcgccaa
     2701 ccccgagcat cagggcgcgt ggctcgacct cgcgcgggca cggagttcgg tcggcgagta
     2761 cgagggcgcc gaacggtgcg cgcgtcgcgc gctcgagtta cacccggagt gcgcggaggc
     2821 gtggttcgag ctcggcaaca cgcagacctc gctcggcgag ctcgacgagg cggtcgggtc
     2881 gtatcgcacc gcgctcgagc tggcgcccga tctgatcgaa gcgtggaaca acctcggcaa
     2941 cacctacgca cgacaggaac gtttcgaaga ggcgatccgc tgctatcgcg aggcggtcga
     3001 acgtcggccg acgacctacc agacctggtt caatctgggt aacgcgcacg gcgtgctgtc
     3061 gcagatgcgc caggcgatct gggcctacga gaacgccgcg agccattccc ccgacaacaa
     3121 tgacgtctgg tacaacctgg cctacgcccg cgcctgctgc gggctgctcg aagcggcggt
     3181 cgacggctac cggcgcgcgg ctcaattgac cccggacgac tacgaggcgt ggttcaacct
     3241 gggccacgcc tacgccgact tggggcgcga cgaacaggcc gccgacgcct atcggaaggc
     3301 gttggccgtc gatcaaaccg atcacgaggc gtggaacaac ctcggcaacg tgctgctgcg
     3361 cctacagcga cccgacgaag cgatcgattg ctaccgccgg gctctcgaga ttaccgcggg
     3421 ctaccatccc tcctggaaca acctgggcaa cgcgctcgag gagcagggcg agctgaacga
     3481 ggccgcggag gcctacgccc gcgcgatcga gcttgccccg cgccggccca cctacctgct
     3541 caaccgcgcg tcggtgctgg cccggatcgg ccgtgccgag gacgccgtcg acgacttggt
     3601 ccacgccctc gacacggcgc ccgagattcg gggtctgctt cccgggttca aggagttcgg
     3661 gtcgctgctg cgtcaccccc gcctgcgccg ctgagctgcc gacgcccccg ggcgttgcac
     3721 cgacccttcg atgatgtatg ttcagctgtg tccggccgag cgacgaccgc cttcacgacg
     3781 cgggtggaat cagatgatcg gtgagaatct ctcgttggaa ctgctggaag atcaggttca
     3841 cggcttggcg gtcgcggagt ctccggccga ggtgttcaag gcattgatcg acggcgcgcg
     3901 catcgcggcg ccacgcactg cggtattcct cgtacgacag gaccagctca agggatgggg
     3961 cacggtcggc tacccggtgg aggccgcgac acgccagcgc gcgtttgtcg gtccgtccga
     4021 ccgggggtgg ttcggcgagg tgctgcgggt cgagcaaccc gtccccggcg tcgatggccg
     4081 cgacgagtat ccggatttcg gccaaccctc gaccgaccag ttcgtcggtc tggcggtgcg
     4141 cgtcaagggc cggccgatcg ccgtgctcgt cggggaacgg acgacgggcc agttgccctg
     4201 gcatccgcag gtgctcggca tcctggtgcg agtggcgcag ttgcggttgg acctcgacct
     4261 cgtccgccgc aagctggcgg ctgccgggac gagtccgggg ccgcccctct cgccgggcgg
     4321 ccccgccgcc ccggggagag gggcgacgag aaagccgagc gtgataccgc cggcgatcac
     4381 gaccgcaccg gtcgagccga ccccggagtc cgtgaccgag gtggccgccg cgacgccggc
     4441 accggtcgag gacggaccgc gcggcgacgc gcgccacgaa gcggcccggc gatacgcccg
     4501 gctcgtcgcc accgacatcc ggctgtacaa cgaagaggc
//
LOCUS       DASPGN010000545         2616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_921961, whole genome shotgun sequence.
ACCESSION   DASPGN010000545 DASPGN010000000
VERSION     DASPGN010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921961"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VD788_15850"
     CDS             <1..880
                     /locus_tag="VD788_15850"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXV77785.1"
                     /translation="APYFLDSSATSTFLKSVVAHEVAHQWWGNRIANANRRNYWFVES
                     LAEYFSAVYMEYAHGWSSYMDQVEGWRRSILESTLKGSVQNADTMFAGEWGGGRSLSP
                     RTAAIYNKGPYAFHILRETFKGQGPRGNDAADKKFFDFLKQATQELAEKREIVTLDIQ
                     RAAEKGLGGIDQNGQPYTVDLSWFFDQWIRGSGLPQLRLDWTSRPAEDGGHVLEGTIY
                     QRVMLGRTDQPLEGRVYRGVVDLKVVAKGGPYLKRLVINGPETPVQLKLPEKPVEVLL
                     NDEGAMLALDTTYGKW"
     gene            894..2381
                     /locus_tag="VD788_15855"
     CDS             894..2381
                     /locus_tag="VD788_15855"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXV77786.1"
                     /translation="MRAGVVAGLALSAGLVLGGAGAAETAAPSGAETFDAAWTIVRDT
                     HFDPGLNGVDWDAVRAELRPKAESASSAEELRGILSDMLGRLGQSHFAIFPQETVDRL
                     ESFRVESVAPAGAPAASEPPGSPEVAGPTAEATAGDVGHSEEADEAEDGSGVVGLEVR
                     LLDGRVVVTRVEADYAAAEQGVRPGWLVTRIDDRDVVELIEFVRGEMAGALYGEQVIV
                     AEVVAEALNGPPGSTIDLGFVDGDGATVERTLERRAAKGRLVRFGNLPPMMTRVDGRS
                     VTTEEDQRVGIVSLSSWMVPAMAELDRLLFEFRDVDGVVFDLRGNPGGVAAMVMGTAG
                     HFIDQRVDLGTMRMRTSSLRFAVNPRLVDGANERVGVFDGPLAILVDGLSASTSEVFA
                     GGMQDVGRARVFGETTMGAVLPAQMERLPNGDVLYHAFAEYVTPSGVALEGRGVVPDE
                     SVRLTRAALLDGRDPQLDAAVRWIVIELNRAPAPLEAASPEEGTQ"
     gene            2381..>2616
                     /locus_tag="VD788_15860"
     CDS             2381..>2616
                     /locus_tag="VD788_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77787.1"
                     /translation="MRYAIVLALMIVVAVGIVAADKQTELPSVEQVIAKHVEAIGGEQ
                     AYLKYDSRTLEGTMSLPTAGLSGVLTAVAMAPDKT"
BASE COUNT          412 a          840 c          965 g          399 t
ORIGIN      
        1 ggctccctac tttctcgact cgtcggcgac ttcgacgttc ctcaaatcgg tcgtcgcgca
       61 cgaggtcgcg caccagtggt ggggcaatcg catcgccaac gccaaccggc gcaactattg
      121 gttcgtcgaa tcgctggccg agtacttctc ggccgtctac atggagtacg cccacggctg
      181 gagctcgtac atggaccagg tcgagggctg gcgccgctcg atcctcgagt cgacgttgaa
      241 gggaagcgtg cagaacgcgg acacgatgtt cgccggcgag tggggtggag ggcgttcgct
      301 cagcccgcgg actgcggcga tctacaacaa gggcccgtat gcctttcaca tcctgcgaga
      361 gacgttcaag gggcaagggc cgcgcggcaa cgacgcggcg gacaagaagt tcttcgactt
      421 cctcaagcag gcgacccagg agctggcgga aaagcgcgag atcgtcacgc tcgacatcca
      481 gcgcgccgca gaaaagggac tgggcggcat cgaccagaac ggccagccct acaccgtcga
      541 cctgagctgg ttcttcgatc agtggatccg cggctcgggg ctgccgcagc tccgtctcga
      601 ctggaccagc cgcccggccg aggacggtgg gcacgtgctc gagggcacga tctaccagcg
      661 ggtgatgttg ggccggaccg atcagccgct cgaaggccgt gtctaccgtg gtgtcgtcga
      721 cctgaaggtc gtggccaagg gcggaccgta cctcaagcgg ctcgtgatca acggcccgga
      781 gacccccgtg cagctcaagc tgcccgagaa accggtcgag gtgctgctca acgacgaggg
      841 cgcgatgctg gcgctcgaca cgacctacgg caagtggtga cggcggatcg cgcatgagag
      901 ccggtgtcgt cgcgggcctg gccctgtcgg cgggactcgt cctcggtggc gcgggcgcgg
      961 cggagacggc cgcgccgtcg ggggccgaga cgttcgacgc ggcgtggacg atcgtccgcg
     1021 acacccattt cgatcccggc ctgaacggcg tggactggga cgccgtgcgg gccgagctgc
     1081 ggccgaaggc cgagagcgct tcctcggccg aggagctgcg cgggatcctg tccgacatgc
     1141 tcggtcggct cggccagtcc cacttcgcca tcttcccgca ggagacggtc gaccgcctgg
     1201 agagcttccg cgtcgagagc gtcgcccccg ccggtgctcc tgcggcgagt gagccgcccg
     1261 ggtcgccgga ggtcgccggg ccgacggccg aagcgaccgc gggcgacgtg ggccactcgg
     1321 aagaagcgga cgaagcggaa gacggcagcg gcgtcgtcgg tctcgaggtg cgtctgctcg
     1381 acggacgggt cgtcgtgacc cgcgtcgagg cggactacgc cgcggccgag cagggggtgc
     1441 gaccgggttg gctcgtcacc cggatcgacg atcgcgacgt ggtcgagctg atcgagttcg
     1501 tgcgcggcga gatggccggt gcgctgtacg gcgagcaggt gatcgtcgcc gaggtggtcg
     1561 ccgaggcgct caacggcccc ccggggtcga cgatcgatct gggtttcgtc gacggcgacg
     1621 gcgcgacggt cgagcgaacg ctcgaacggc gggcggccaa gggccggctg gttcggttcg
     1681 gcaacttgcc gccgatgatg acacgcgtcg acggacgcag tgtcacgacc gaggaggacc
     1741 agcgtgtcgg catcgtctcg ctcagctcgt ggatggtccc ggcgatggcc gagctcgacc
     1801 gcttgctgtt cgagtttcgc gacgtcgacg gcgtcgtctt cgacctgcgc ggtaatcccg
     1861 gcggcgtcgc cgcgatggtg atgggcaccg ccggtcactt catcgaccag cgcgtcgacc
     1921 tgggcacgat gcgcatgcgc acgagctcgc tgcggttcgc ggtcaacccg cgactcgtcg
     1981 acggcgcgaa cgagcgcgtc ggcgttttcg acggaccgct cgcgattctg gtcgacggtc
     2041 tgtcggcgag cacctccgaa gtgttcgccg ggggcatgca ggacgtcggt cgcgcccggg
     2101 tcttcggcga aacgacgatg ggcgccgtgc tgccggcgca aatggagcgc ctacccaacg
     2161 gcgacgtgct gtaccatgcg tttgccgagt acgtcacgcc gtcgggggtc gcgctcgagg
     2221 gccggggtgt cgtacccgac gagtccgtgc ggctgacgcg ggcggcgcta ctcgacggtc
     2281 gcgacccgca gctcgatgcc gccgtccgct ggatcgtgat cgaattgaac cgggcccccg
     2341 cgccgctcga ggcagcgtcg cccgaggaag gaacgcagtg atgcgatacg caatcgtgct
     2401 cgcgctgatg atcgtcgtcg cggtcggaat cgtggcggcc gacaagcaga ccgagctgcc
     2461 gagcgtcgag caggtgatcg ccaagcacgt cgaggcgatc ggcggcgagc aggcctacct
     2521 gaagtacgac agccggacgc tcgaagggac gatgtcgctg ccgacggcgg ggttgtccgg
     2581 tgtgctgacg gccgtggcga tggccccgga caagac
//
LOCUS       DASPGN010000546         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_570868, whole genome shotgun sequence.
ACCESSION   DASPGN010000546 DASPGN010000000
VERSION     DASPGN010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570868"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VD788_15865"
     CDS             <1..571
                     /locus_tag="VD788_15865"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77788.1"
                     /translation="PDVRISHIYASTEAGVGFSVNDRKPGFPLGFLSAPPSGIELRVV
                     DNRLFVRNRQVDSHYVGTDSSIVDPEGWVDTGDVVETREDRVYFLGRASGVINVGGNK
                     VHPERVERVLLSHPSVVQARAFAKSNPLSGALVAAEVVLTEGTPATTETRQALLTHAG
                     RQLGRHEVPVSIRFVDRLEMNAVGKMVRR"
     gene            573..1295
                     /locus_tag="VD788_15870"
     CDS             573..1295
                     /locus_tag="VD788_15870"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV77789.1"
                     /translation="MKNVLLTGATRGLGIAIAARLVRDGYRVAGVGRVRSERFAELEQ
                     IDGPGVAEFVPYDLAELEGIPSLVSDVVRRHGALYGVVNNAGIGVDGVLATMHATDIS
                     RVLRVNLEAAIVVAKYGCRSMLTRGEGRIVNVSSIIASTGFSGLSVYAASKAGLEGFT
                     RSLARELGRAGITVNCVAPGYMETDMTRGLDDRKLETVRRRAPLGLPLTEDVAAAVAH
                     LLSDDAKRTTGTILTVDGGATA"
     gene            1292..2389
                     /locus_tag="VD788_15875"
     CDS             1292..2389
                     /locus_tag="VD788_15875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77790.1"
                     /translation="MTIKLELEVASVTDEAAIVAFIDRYWRKNHVFVSNPELLRWQHQ
                     ADDRPEERLNFVIARRERGDGAPPELLGLLGFIPFRRFDRDADWTDLSLAIWKVRDDA
                     PPGLGLRLLKEVERQRSPALICAIGISDMVKPIYGALGYSLASLTHAALFDPSRRPDS
                     CVADGVPDAAWRDLRSDERIELRPLSARELERGGLAEEIDRLGAAASPRKSSSYVLNR
                     FCRHPWYAYDVRAVLADGRAEAVLVWRRVTAMQGAVLRIVDLVGNAEIVARCGPALRQ
                     AVQAAGCEYVDLVCYGVPGDALRRGGFVSTSDHPGLVLPNYFDPFERRNVVIDIAWRR
                     RAGEGGQPVRLFRADSDQDRPNSSASLPERQ"
     gene            complement(2407..3375)
                     /locus_tag="VD788_15880"
     CDS             complement(2407..3375)
                     /locus_tag="VD788_15880"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXV77791.1"
                     /translation="MRAVMYHYVRETPAAMPHFRYLHADNFRRQLDRFESEYRFVTRD
                     EWTDSINRGAPIDDGILLTFDDGLSDHFEFVMPELIRRGLWGLFYVSTAPYRRHRLLD
                     VHRIHCLLGTLGGVELLRRLRRLVDQSMLSGRAVQVFSERSYTLQGNHEATVECKRIL
                     NYYLSYDELQTVLDRLMLDSPLDETELVRRFYLGVEQIGEMHRAGMWIGSHSVNHRVM
                     SRLSEAEQSREIRESFEFLEELLDEPPPRSFCYPYGIPNSFDATTERLLDRAGCDFSF
                     SAQPGSITRQDLLSRRQALPRYDCNQLPHGTAHYGDRAMPADSSPR"
     gene            complement(3379..>3631)
                     /locus_tag="VD788_15885"
     CDS             complement(3379..>3631)
                     /locus_tag="VD788_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77792.1"
                     /translation="FGKRKALMHGVDRSERHVCKIFVTDMHTLKAYGNTQIEVQKEGF
                     ENLFVFHNQHRGEPMETRWPSRSKACRNQGKRRMSSVAG"
BASE COUNT          623 a         1142 c         1198 g          668 t
ORIGIN      
        1 tcccgacgtc cgtatctcgc acatctacgc ctcgaccgag gcaggtgtcg gcttctcggt
       61 caatgaccgg aagccgggtt ttccactcgg cttcctctcc gccccgccgt cgggaatcga
      121 gctgagggtg gtcgacaatc gcttgttcgt acggaatcga caggtcgaca gccactacgt
      181 cggtaccgac agttcgatcg tcgatccgga aggttgggtc gacacggggg acgtggtcga
      241 gacacgggaa gaccgcgtct attttctcgg tcgagcgagc ggagtcatca acgtcggcgg
      301 caacaaggtg catcccgagc gagtcgaacg ggtgttgttg tcccacccct cggtcgtcca
      361 ggcccgtgcg ttcgccaagt ccaatccgct cagcggggcg ctcgtcgcgg cggaagtcgt
      421 gctaacggag gggaccccgg cgactaccga gacccgtcag gcgctgctga cccacgccgg
      481 ccggcagctg ggacgccacg aggttccggt cagcatccgg ttcgtcgacc gactggaaat
      541 gaatgccgtg ggaaagatgg tgaggcgctg agatgaagaa cgtcttgctg accggagcga
      601 cacgcggcct cggaatcgcc attgccgcac ggctggtacg ggacggctac cgagtcgcgg
      661 gggtgggccg cgtgcggagc gagcgatttg ccgaactgga gcagatcgac ggtccgggtg
      721 tcgccgagtt cgtcccgtac gatctcgccg aactggaggg gattccgtcg ctcgtgtccg
      781 atgtcgttcg acgacacggt gcgttgtatg gggtggtcaa caacgccggc atcggcgtcg
      841 acggcgtgct ggcgacgatg cacgcgaccg acatatccag ggttcttcgt gtgaacctgg
      901 aggccgcgat cgtcgtcgcc aagtacggtt gcaggagcat gctgacccgc ggtgagggca
      961 ggatcgtcaa cgtttcgtcg atcatcgcgt cgaccggatt cagcgggctg tcggtctacg
     1021 ccgcgtcgaa ggccggcctg gaggggttca cccgctcact cgcccgcgag ctcggccgcg
     1081 ccggaatcac ggtcaactgc gtcgctcccg gatatatgga aaccgacatg acgcgcggtt
     1141 tggacgatcg caagctcgag accgtcagac gacgcgcccc tctcgggttg ccgctcaccg
     1201 aagatgtcgc cgcggccgtc gcgcacctgt tgtcggacga tgcgaaacga acgaccggta
     1261 cgatcctcac ggtggacgga ggcgcgaccg cgtgacgatc aagctcgagc tcgaagtcgc
     1321 ctccgtcacg gacgaggcgg cgatcgtcgc tttcatcgat cgatactggc gcaagaatca
     1381 cgtgttcgtc tcgaacccgg agttgctgcg ttggcagcat caggccgacg atcgacccga
     1441 ggagcgattg aacttcgtca tcgcccgtcg cgagaggggt gacggcgctc cgccggagct
     1501 gctgggcctg ctcgggttca ttccattccg gcgcttcgat cgcgacgccg actggacgga
     1561 tttgtcgctc gccatctgga aagtccgcga cgatgcgcca ccggggctcg gtctgcgttt
     1621 gctcaaggag gtcgagcggc agcggtcgcc ggccttgatc tgcgcgatcg gcatcagtga
     1681 catggtcaag ccgatctacg gtgcgctcgg ctactcgttg gcgtcactga cccacgcggc
     1741 cttgttcgat ccgtcgcggc gacccgattc gtgcgtagcg gacggcgttc cggacgcggc
     1801 ttggcgcgac ctgaggtcgg acgaaaggat cgagctgcgc cccctgagcg cacgcgagct
     1861 ggagcgcggc ggtctggccg aggagatcga tcgactcggt gcagccgcct ctccgcgaaa
     1921 atcctcgagc tacgtgctga accgcttctg tcgtcatcca tggtacgcgt acgatgtccg
     1981 cgccgtgctc gccgacggcc gagcggaggc ggtgttggta tggcgtcgcg tcacggcaat
     2041 gcaaggtgcg gtgctgcgca tcgtcgacct cgtcggtaac gccgagatcg tggcccgctg
     2101 cggtccggcg ctacgacagg ccgtgcaggc cgcggggtgc gagtatgtcg acctggtgtg
     2161 ctacggagtg ccgggagacg cgctgcgccg aggcggattc gtatcgacga gcgatcaccc
     2221 cggtctggtg ttgcccaact acttcgaccc gttcgagcgg cgcaacgtgg tgatcgacat
     2281 cgcttggcgt cggcgcgccg gcgagggagg ccagccggtg cgactgtttc gagcggactc
     2341 ggaccaggat cgacccaaca gcagcgcgtc gttgcccgaa cggcagtagg ggagcgtgcc
     2401 gcggcgtcaa cgcgggctcg aatcggccgg catcgcccga tcaccgtagt gtgcggtgcc
     2461 gtggggaagc tgattgcagt cgtatcgcgg aagcgcctgc cgacggctca gcagatcctg
     2521 gcgcgtgatc gatccgggtt gggcactgaa cgagaagtcg cagcccgccc ggtccagcag
     2581 tcgctcggtc gtagcgtcga aagagttcgg aatcccgtac gggtagcaga agctccgtgg
     2641 cggcggctcg tccagcagct cttcgagaaa ctcgaacgac tcgcggatct cgcgggactg
     2701 ctccgcttcg ctcaaccgac tcatcacccg atggttgacc gaatggctgc cgatccacat
     2761 tcccgcgcgg tgcatctcgc cgatctgctc gacaccgagg tagaaccggc gcacgagctc
     2821 cgtctcgtcc aacgggctgt ccagcatcaa tcggtccagt acggtctgca actcgtcgta
     2881 cgagaggtag taattgagga ttcgcttgca ctcgaccgtc gcctcgtggt tgccctgcaa
     2941 cgtgtagctc cgttccgaaa acacttgcac ggcccgcccc gacagcatcg actggtccac
     3001 cagccgtctc agccgacgca gcagctcgac cccgcccagc gttcccagca ggcagtggat
     3061 gcgatgtacg tccagcagtc tgtgtcgtcg atacggagcc gtcgagacgt agaacagccc
     3121 ccacaacccg cgtcgtatca gttccggcat cacgaactcg aagtggtcgc tcagcccatc
     3181 gtcgaacgtc agcaagatgc cgtcgtcgat cggggcccct cgattgatcg aatcggtcca
     3241 ctcatcgcgc gtcacgaagc ggtactccga ctcgaagcga tccaactgcc gacgaaagtt
     3301 gtccgcgtgc agataacgga agtgcggcat cgcggcggga gtctcgcgca cgtagtggta
     3361 catgacggcg cgcatcgctc aacccgcgac agaactcatc cgtcgtttcc cttggtttcg
     3421 acaggccttc gatcgtgatg gccagcgcgt ctccatcggt tcaccgcggt gctggttgtg
     3481 gaagacgaac agattctcga aaccttcttt ctggacctcg atttgtgtgt tgccgtacgc
     3541 tttcagcgtg tgcatgtcgg tgacgaagat cttgcagacg tgacgctcgc tgcggtcgac
     3601 gccgtgcatc agcgccttcc gcttgccgaa a
//
LOCUS       DASPGN010000547         2203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_338150, whole genome shotgun sequence.
ACCESSION   DASPGN010000547 DASPGN010000000
VERSION     DASPGN010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338150"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..458)
                     /locus_tag="VD788_15890"
     CDS             complement(51..458)
                     /locus_tag="VD788_15890"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXV77793.1"
                     /translation="MLNVLRENLKKRAWTKWLLLLVAGSMTLYLAAYFGGSGGDEAPR
                     ADGNWAARIDGEAIPSQHYLQLARRIDQTYRQMFGANYNDMRDQFQIGTQAMRQIIEQ
                     EIILRDARRLGLSISDEELIEPAPRRPRAPCGP"
     gene            619..2073
                     /locus_tag="VD788_15895"
     CDS             619..2073
                     /locus_tag="VD788_15895"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HXV77794.1"
                     /translation="MARETTSTFQIEWFNVTYRSVFSVLGVVVLLVGGGAAYWYYFHV
                     HAPRAAAVDAIARAESKVAEAAALDSSDDRLADVVASAQVALREARQAFGVLRFDEAR
                     AAAIRSENLSQQAIGLVKGVDLDARMVRFYRLEGDVRIKRAGQFSWESASTRTELAEG
                     DQIKTSSKGSAEVIFFDGSITRIEPGSLYKIREVQENPHTKERRVREELAFGELKAST
                     QKRNVEGSFHEVKAGEIEARAEEATEFRVTAEEGSKKAAVDVFQGQIEVASSDRRENV
                     VAGERISAGADGKLRDKEVLPGIPALVSPRDQKVFILAPDRPQPITLAWDSVPGAERY
                     HLLISDKTLFTEPLYDAERNGSSALIDEVPEGAYYWKVAAIGANGVRGPFSAARRFRV
                     SSHKILDRSDTEPPSLEITEFVAVGPMVVVNGRTEPGATLWVDNEKQEVFDDGQFSTV
                     VRLRRDGMNEIRFVAQDTAGNETELTRAAYVEVY"
BASE COUNT          376 a          717 c          767 g          343 t
ORIGIN      
        1 gacgacgtcc gggtgctcga acgtgttgtg cgcgtcgagc gcgggtgcgg tcagggtccg
       61 cacggtgcac gaggtcggcg cggcgccggc tcgatcaact cctcgtctga gatgctcaga
      121 ccgaggcgcc gcgcgtcgcg caggatgatc tcctgttcga tgatctgccg catggcttgg
      181 gtgccgatct ggaactgatc gcgcatgtcg ttgtagttcg caccgaacat ctgccggtag
      241 gtctggtcga ttcgccgcgc caactgcagg tagtgctggc tcgggatcgc ctcgccgtcg
      301 atccgcgccg cccagtttcc gtccgcccgc ggagcctcgt cgccgccgct gccgccgaag
      361 taagccgcga gatacagggt catcgagccc gcgaccagca acagcagcca tttggtccag
      421 gctctcttct tgaggttctc tcgaagcacg ttgagcatgg ctcgatctcc ggcgaacagc
      481 ccgaaaacgc gagattctac tgcagccacc ccggtccggc aactgcgcgc cgcaacggcc
      541 gcggacgcct ggccgaccgg ccggtcagac actacattcg ccccggccga gattctggcg
      601 gcggaggcac cccgagcgat ggctcgagag acgaccagta cgttccaaat cgagtggttc
      661 aacgtcacgt accgcagcgt cttctcggtc ctgggcgtcg tcgtgctgct cgtcggcggt
      721 ggcgccgcct actggtacta ctttcacgtc cacgcgccgc gggcggcggc agtggacgcg
      781 atcgcccgcg ccgagtcgaa ggtcgcggag gccgccgcgc tcgacagttc cgacgaccgc
      841 ctcgccgacg tcgtcgccag cgcccaggtt gcgctccgcg aggcgcggca ggcgttcggc
      901 gtgctccggt tcgacgaggc acgcgccgcc gcgatccgct ccgagaacct ctcgcagcag
      961 gcgatcggcc tggtgaaggg cgtcgacctc gacgcgagga tggtgcgctt ctatcggctc
     1021 gaaggcgacg tgcgcatcaa gcgcgccggt cagttctcct gggagagcgc cagcacgcgg
     1081 acggagctgg ccgagggaga ccagatcaag acctcgtcca agggctcggc cgaagtcatc
     1141 ttcttcgacg gcagcatcac gcggatcgag cccggctcgc tgtacaagat ccgcgaagtg
     1201 caggaaaacc cgcacaccaa ggaacgccgg gtccgggaag agctggcgtt cggcgagctc
     1261 aaggcgtcga cgcagaagcg gaacgtcgag ggatcgttcc acgaggtcaa ggccggagag
     1321 atcgaggccc gcgcggagga ggcgaccgag ttccgcgtca ccgcggagga agggagcaag
     1381 aaggccgcgg tcgacgtctt ccagggacag atcgaggtgg catcgagcga ccggcgggag
     1441 aacgtcgtgg ccggcgagcg gatcagcgcc ggcgccgacg gcaagctgcg cgacaaggaa
     1501 gtcctgccgg ggatcccggc gctcgtctca ccacgggacc agaaggtgtt catcctcgcg
     1561 ccggaccgcc cgcagccgat cacgctggcg tgggactccg ttcctggggc cgaacgctat
     1621 cacctgctga tctccgacaa gacgctgttc acggagccgc tctacgacgc ggagcgcaac
     1681 ggctcgagcg cgctgatcga cgaggtgccg gagggagcct actactggaa ggtggcggcg
     1741 atcggggcca atggcgtccg cgggccgttc agcgcggcgc gtcgcttccg cgtctcctcg
     1801 cacaagatcc tcgaccggtc ggacacggag ccgccatcgc tcgagatcac cgagttcgtc
     1861 gcggtcggcc cgatggtcgt ggtcaacggt cggacggagc cgggggctac cctgtgggtg
     1921 gacaacgaaa agcaggaggt gttcgacgac gggcagttct cgacggtcgt ccggctgagg
     1981 cgggacggga tgaacgagat ccgcttcgtc gcccaggata cggcgggaaa cgagaccgag
     2041 ctgacgcggg ccgcatacgt cgaagtatac taattgcata tcttccgcgt tttccttata
     2101 tatacgtctg gagaccgaaa acgcgtcgca cccgtctcgc cgcgggtcgc ggcccgatac
     2161 gggagcctgg gccaaggtgc ggtcgggttt gcctggacgc gcc
//
LOCUS       DASPGN010000548         6178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1507455, whole genome shotgun sequence.
ACCESSION   DASPGN010000548 DASPGN010000000
VERSION     DASPGN010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1507455"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /gene="pyrF"
                     /locus_tag="VD788_15900"
     CDS             complement(<1..662)
                     /gene="pyrF"
                     /locus_tag="VD788_15900"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXV77795.1"
                     /translation="MTRSGRERILVAVDTPELDRARGLVDLLGGHVGGFKIGLELFVS
                     HGPRFVEAIVGGGSSVFLDLKLHDIPNTVAGAARAAGRLGVAFLTMHASGGVPMMRRA
                     VEAANQGAESAGLAPPLALAVTVLTSLDDDELARVGLAGGCGSAVTRLAGLAREAGVG
                     GIVCSPREIGAVRRVFPQARLVVPGVRPAGAPVLGDDQSRVATPGTAVAAGADYLVIG
                     RP"
     gene            complement(659..1594)
                     /locus_tag="VD788_15905"
     CDS             complement(659..1594)
                     /locus_tag="VD788_15905"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HXV77796.1"
                     /translation="MVTPELTVELGPLRLKNPVLTASGTFGYGLEFLPYFDLGRLGGL
                     VTKGLSPRPRAGNPPARICETPAGMLNTIGLQNVGVDHFIEVKLPQLVRYDTAIVANV
                     FGETEHEYVEVCSKLDGAEGLAAIELNVSCPNVERGGMIFGNEPGALRRVTRACRAAT
                     SLPLIVKLSPNVTDIRETARAAADGGADVLSLVNTFVGMAIDVERRRPVLAKVSGGLS
                     GPAIRPLAVWMTWQVHQAVELPLIGIGGIVDADDALQFILAGASAVQVGTANFIHPDA
                     ALRVLAGLEAYLTRHGIASIGELVGALDTDAGIPV"
     gene            complement(1588..2400)
                     /locus_tag="VD788_15910"
     CDS             complement(1588..2400)
                     /locus_tag="VD788_15910"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HXV77797.1"
                     /translation="MPQDVTTPVLRNIDLGHGNYLVEFSSPSMTAGMKPGQFFMIGIP
                     GSETLLRRPYSVCGLPGTFDDAPRDSVRVLYKVVGRGTALLAALKSGAPLTVLGPLGN
                     GFEAPSPAAVRPVFVAGGIGSAPFPALARWLDGRCRPPWMFYGARSAIDLPLLDWFRR
                     HADEVVTTTEDGTAGRRGLVTEPLIEFLDSTDAGELHLYACGPEPMLRAVAALARRRG
                     LRCDLSLEAHMACGFGVCLGCVVPTTTDGSHETRYDRVCVEGPVMEAGRLAW"
     gene            2562..3152
                     /gene="pyrR"
                     /locus_tag="VD788_15915"
     CDS             2562..3152
                     /gene="pyrR"
                     /locus_tag="VD788_15915"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003547.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HXV77798.1"
                     /translation="MPAAKSVRIMDAVSIDRALTRIAHEILERNRTPERLSLVGIRTR
                     GVPVAMRTAAKIAEIRGSAPPTGMLDINLYRDDLSLIAATHPVLRRTEIPFDVDGARI
                     VLVDDVLFTGRTIRAALDGLMDLGRPDSIQLAVLVDRGHREVPIKADYVGKNVPTSRS
                     ETIEVRMKEVDGKDEVRLLRTAADEDRPNRRGGDGR"
     gene            3149..4087
                     /locus_tag="VD788_15920"
     CDS             3149..4087
                     /locus_tag="VD788_15920"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXV77799.1"
                     /translation="MRLRSRHLLGIDGMTTGEIELILDTADSLSEIATRDIKKVPTLR
                     GRTVVNFFVEPSTRTRSSFELAEKRLSADTINFSASGSSFVKGETLLDTTRTLESMAP
                     DFIVIRHSQPGAPHFLARHGRCSIINAGDGAHEHPTQALLDAYTIRKHKGRINGLRIA
                     IIGDILNSRVARSNLFLLATMGADVVLCGPPTLIPMHVQRFNTAVTHRVDQAVEGADV
                     VMALRIQRERMRGGAFPSLREYAKLYGINRERLARAAPDAIVMHPGPMNRGIEITGEV
                     ADGERSVILDQVANGVAVRMAVLYLLAGELGREAAA"
     gene            4071..5348
                     /locus_tag="VD788_15925"
     CDS             4071..5348
                     /locus_tag="VD788_15925"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXV77800.1"
                     /translation="MKLLLENGRVVDPASGVDGPRDVLIDGGKIVAVERRIDGRGAER
                     IDLDGLVVCPGFIDMHVHLREPGQEWKETVESGTAAAAAGGFTGVACMGNTQPVNDSR
                     SVTELIMTQAAEFGSVPVYPIGCVTKGQLGEELAEMDDMREAGAVAFSDDGRPVRSSL
                     MMRKALEYSRIFDVPIIDHCEDTELVDGGVMDEGEMSTRLGLKGWPAVAEDIIVQRDI
                     LLAEYTEGSVHIAHISTAGAVEFVRRAKKKKIRVTCEVTPHHLLLEDSAVGDYDTDAK
                     MNPPLRGARHRKAMLKAIADGTIDAIATDHAPHHTDEKCVEFSLAPFGVVGLETAVSL
                     CLDRLVAAGVIDLSRMVELFTIGPARILRLAKGTLVPGADADVTVLDLKRRKKVVPAE
                     FRSKSRNSPFAGWELAGAPLMTFVGGRLVFDGR"
     gene            5371..>6178
                     /locus_tag="VD788_15930"
     CDS             5371..>6178
                     /locus_tag="VD788_15930"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXV77801.1"
                     /translation="MPTVYETRLPEVRLVARGKVRDIYDLGQRLLLVASDRLSAFDYV
                     LPTPIPDKGKVLTQISVFWFERFSDLVANHMVTTDVAEFPSPLPAYAAQLEGRSMLVE
                     KLEMLPVECVARGYLAGSGWTEYRERGTVCGIKLPAGLEESAPLPEPIFTPATKAQSG
                     HDENIPYGEVERLIGEERAAEVRKLTLDLYRAGSEYARQQGILIADTKFEFGVAHDRL
                     VLADEALTPDSSRFWPVAGYRPGGSQPSFDKQYVRDHLLSIGWNKQPPVPR"
BASE COUNT         1030 a         2057 c         2201 g          890 t
ORIGIN      
        1 atcggccggc cgatcaccag gtagtcggcg ccggcggcga cggcggtacc cggcgtggcg
       61 acacgcgatt ggtcgtcacc gagcaccggc gccccggcgg gacggacccc gggaacgacc
      121 aggcgcgcct ggggaaagac ccggcgcacc gcgccgatct cgcgcggcga gcagacgatc
      181 ccgcccacgc cggcctcgcg cgcgagcccg gcgagccgcg tcaccgccga gccgcaccct
      241 ccggcgagac cgacccgagc cagctcgtcg tcgtcgaggc tcgtcagcac ggtgacggcg
      301 agcgcgagcg ggggagccaa cccggcggac tcggcaccct ggttcgccgc ctcgaccgcg
      361 cgccgcatca tcggcacacc gcccgacgcg tgcatcgtga ggaacgccac gccgagacgg
      421 ccggcggcgc gcgcggcgcc ggcgacggta ttgggaatgt cgtgcagctt gagatcgagg
      481 aagacgctcg acccgccgcc gacgatcgcc tcgacgaacc gcggaccgtg gctgacgaac
      541 agctcgagcc cgatcttgaa gccgccgacg tgaccaccga gcaagtcgac cagcccgcgt
      601 gcgcgatcga gctccggcgt atcgacggcg acgaggatcc gttcgcggcc ggaccgggtc
      661 aaaccgggat tccggcgtcg gtgtcgagtg cgccgaccaa ctcgccgatc gacgcgatgc
      721 cgtggcgggt caggtaggcc tcgagacccg cgagcacgcg gagcgccgcg tccgggtgga
      781 tgaagttggc ggtgccgacc tgcaccgcac tggcgccggc caggatgaac tgcagggcgt
      841 cgtcggcgtc gacgatgccg ccgataccga tcagcgggag ctcgaccgcc tggtgcacct
      901 gccacgtcat ccagaccgcg agcggtcgga tcgcgggacc cgacagacca cccgagacct
      961 tggccagcac cggtcgccgc cgttccacgt cgatcgccat cccgacgaag gtgttgacca
     1021 gcgacagcac gtcggcaccg ccgtcggccg ccgcccgtgc cgtttcgcgg atgtcggtca
     1081 cgttgggcga gagcttgacg atcaacggga gcgaggtggc ggcgcgacag gcccgcgtca
     1141 cccgacgcag ggcgcccggc tcgttgccga agatcatccc gccccgttcg acgttggggc
     1201 aggagacgtt gagctcgatc gccgccaggc cctcggcgcc gtcgagcttg gagcacacct
     1261 cgacgtactc gtgctcggtc tcgccgaaga cgttggcgac gatcgcggtg tcgtagcgca
     1321 cgagctgcgg cagcttgact tcgatgaagt ggtcgacgcc gacgttttgc agaccgatcg
     1381 tattcaacat gcccgccggg gtctcacaga ttcgcgccgg cgggttgccg gcgcgcggcc
     1441 ggggcgacag gcccttggtg accaacccac ccaaccgtcc caggtcgaag tacggcagga
     1501 actcgagccc gtagccgaag gtccccgagg cggtcagcac cgggttcttg agtcgcagcg
     1561 ggccgagctc gactgtcagc tcgggggtca ccatgccaac cgcccggctt ccatcaccgg
     1621 tccctcgaca cagacccggt cgtaccgcgt ttcgtgcgag ccgtcggtgg tcgtcggtac
     1681 gacgcagccg aggcagacgc cgaagccgca ggccatgtgg gcttcgagcg acaggtcgca
     1741 gcgcaggccg cggcgccggg cgagcgccgc cacggcgcgc agcatcggct cggggccgca
     1801 ggcatacagg tggagctcgc ccgcgtcggt cgagtcgagg aactcgatca atggctcggt
     1861 gaccaggccg cgtcgtccgg cagttccgtc ctcggtcgtg gtcacgacct cgtcggcgtg
     1921 gcgccggaac cagtcgagca acggcaggtc gatcgcgctg cgcgcgccgt agaacatcca
     1981 gggcggccgg cagcggccgt cgagccagcg cgcgagcgcg gggaacggtg cggagccgat
     2041 cccaccggcg acgaagaccg gccgcacggc ggccggggac ggagcctcga agccgttgcc
     2101 cagcggcccg agcacggtca gcggtgcgcc gctcttgagc gccgcgagca acgccgtgcc
     2161 gcgaccgacg accttgtaca gaactcgaac cgaatcgcgt ggcgcgtcgt cgaacgtccc
     2221 gggaagacca cagacgctgt acggccggcg cagcagcgtt tcgctgcccg ggatgccgat
     2281 catgaagaac tgacccggct tcatcccggc cgtcatcgac ggtgaggaga actcgacgag
     2341 gtagttccca tggccgagat cgatgttgcg aaggacggga gtcgtgacgt cttggggcat
     2401 gaaggacgat ccgtgcgaaa ggatagaccg ccgtgcggaa ccggtcaacg gccggccgcg
     2461 cggcttgacc ggccgcgtcg ctttggctag catacgccga cccggagatt ccgtgacgcc
     2521 cctttaagcc gaccccgaga ggtcggcaag gagcctgctc gatgcccgcc gccaagagcg
     2581 ttcggatcat ggacgcggtg tcgatcgatc gcgcgctgac acggattgcc cacgagatcc
     2641 tcgaacggaa ccgcacgcca gagcgcttga gcctcgtcgg catccgcacg cgcggtgtcc
     2701 cggtcgcgat gcgtaccgcg gccaagatcg ccgagatccg cggcagcgca ccaccgaccg
     2761 gaatgctcga catcaacctc tatcgtgacg acctgtcgct gatcgccgcg acccaccccg
     2821 tgctgaggcg gaccgagatc ccgttcgacg tcgacggcgc acggatcgtg ctcgtcgacg
     2881 acgtgctgtt caccggacgg acgatccgcg ctgcgctgga cgggctgatg gacctcggtc
     2941 gcccggactc gatccaactc gcggtgctgg tcgaccgcgg ccaccgcgag gtgccgatca
     3001 aggccgacta cgtcggcaag aacgtgccga cctcgcgcag cgagacgatc gaggtgcgga
     3061 tgaaagaggt cgacggcaag gacgaggtgc gcctgctgcg caccgcggct gacgaagacc
     3121 ggccgaatcg ccggggcggt gacgggcgat gaggttgcgg agccgacatc tgctcgggat
     3181 cgacgggatg acgaccggcg agatcgagct gatcctcgat accgccgact cgctgtccga
     3241 gatcgcgacg cgcgacatca agaaggtgcc tacgcttcgc ggcaggacgg tggtgaactt
     3301 cttcgtcgag ccgtcgacgc ggacccggtc gtcgttcgag ctggcggaga agcggctgtc
     3361 ggcggacacg atcaacttct ccgcctccgg ttcgagcttc gtcaagggtg aaacgctgct
     3421 cgacacgacg cggacgctcg agtcgatggc ccccgatttc atcgtgatcc ggcactcgca
     3481 acccggcgcg ccgcacttcc tggcgcggca cgggcgctgc tcgatcatca acgccggtga
     3541 cggcgcgcac gagcacccga cgcaggccct gctcgacgcc tacaccatcc gcaagcacaa
     3601 gggtcggatc aacgggctgc gtatcgcgat catcggcgac atcctcaata gccgcgtggc
     3661 ccgctcgaac ctgttcctgt tggcgacgat gggggccgac gtcgtgctgt gcggtccgcc
     3721 gacgctgatc ccgatgcacg tgcagcggtt caacaccgca gtgacgcacc gcgtcgacca
     3781 ggcggtcgaa ggggcggacg tggtgatggc gttgcggatc cagcgcgagc ggatgcgggg
     3841 cggggcattc cccagcctgc gcgagtacgc gaagctctac ggcatcaatc gcgaacgact
     3901 ggcgcgcgcc gcgcccgatg cgatcgtgat gcacccgggc ccgatgaacc gcgggatcga
     3961 gattacgggc gaggtggcgg acggcgagcg atcggtcatt ctggatcagg tggccaacgg
     4021 ggtcgcggtc cggatggccg tgctgtactt gctggcggga gagctcggtc gtgaagctgc
     4081 tgcttgagaa cggtcgcgta gtcgatccgg cgtcgggggt cgacggcccg cgtgacgtgc
     4141 tgatcgacgg cggcaagatc gtggcggtcg agcggcgcat cgacgggcgg ggtgccgagc
     4201 ggatcgacct ggacgggctc gtcgtctgcc cgggtttcat cgacatgcac gtccacctgc
     4261 gtgagcccgg ccaggagtgg aaggagacgg tcgagagcgg tacggcggcc gccgccgccg
     4321 gaggcttcac cggcgtcgcc tgcatgggga acacccagcc ggtcaacgac tcgcgatcgg
     4381 tcaccgagct gatcatgacg caggccgcgg aattcggcag cgttccggtc tatccgatcg
     4441 gctgcgtgac caagggacag ctcggcgagg aactggccga gatggacgac atgcgcgagg
     4501 ccggtgccgt cgcattctcc gacgacggcc ggccggtgcg cagcagtctg atgatgcgca
     4561 aggcgctcga gtactcgcgg atcttcgacg tgccgatcat cgatcactgc gaagataccg
     4621 agctcgtcga cggcggggtg atggacgagg gcgagatgtc gacgcggctc ggcctcaagg
     4681 ggtggccggc ggtcgccgag gacatcatcg tgcagcgcga catcctgctc gccgagtaca
     4741 ccgaaggctc cgtgcacatc gcgcacatct cgacggcggg cgccgtcgag ttcgtgcgcc
     4801 gggccaagaa gaaaaagatc cgcgtcacct gcgaggtgac gccgcaccac ttgctgctcg
     4861 aggacagcgc ggtcggcgac tacgacaccg acgccaagat gaacccaccg ctgcgcggcg
     4921 cacgtcatcg caaggcgatg ttgaaggcga tcgccgacgg gacgatcgac gcgatcgcga
     4981 ccgaccacgc tccgcatcat accgacgaga agtgcgtcga gttctcgctg gcgccgttcg
     5041 gggtcgtcgg tctcgagacc gcggtgtcgc tgtgtctcga ccgactggtc gccgccggcg
     5101 tgatcgactt gtcacggatg gtcgagttgt ttacgatcgg gccggcgcgg atcctgcgat
     5161 tggccaaggg gacgctggtg cccggcgccg acgccgacgt gaccgtactc gatctgaagc
     5221 gccggaagaa ggtggttccc gcggagttcc gctcgaagag tcgcaattcg ccgttcgccg
     5281 gctgggagct cgcgggcgcg ccgctgatga cgttcgtcgg cggccggttg gtcttcgacg
     5341 gacgctaggg gattcgggaa aggaggagac gtgccgacgg tatacgaaac ccggctgcct
     5401 gaagtccgcc tcgttgcgcg tggcaaggtg cgggacatct acgacctggg ccagcggctg
     5461 ctgctcgtcg cgagcgaccg gctgtcggcc ttcgactacg tgctgccgac gccgattccc
     5521 gacaagggca aggtgctgac gcagatctcc gtcttctggt tcgagcggtt ctcggacctc
     5581 gtcgccaatc acatggtgac gacggacgtc gccgagttcc cgtcgccgtt gccggcctat
     5641 gccgcgcagc tcgagggtcg gtcgatgctg gtcgagaagc tcgagatgct gccggtcgaa
     5701 tgcgtggcgc gaggctacct cgccgggtcg ggctggacgg agtatcgcga gcgggggacg
     5761 gtttgcggga tcaagctgcc ggccgggctc gaggaatccg cgccgctgcc cgagccgatc
     5821 ttcaccccgg cgaccaaggc gcagagcgga cacgacgaga acatcccgta cggcgaggtc
     5881 gagcggttga tcggcgagga gcgcgcggcc gaggttcgca agctgacgct cgatctgtat
     5941 cgggccggct cggagtacgc ccgccagcag ggcatcctga ttgccgacac caagttcgag
     6001 ttcggcgtgg cgcacgaccg gctggtgctg gccgacgagg cgctgacgcc ggattcgtcg
     6061 cgcttctggc cggtcgcggg ctaccgaccc ggcggaagcc agccatcgtt cgacaagcaa
     6121 tacgtccgcg accatctgct gtcgatcggc tggaacaagc agccgccggt gccgcggc
//
LOCUS       DASPGN010000549         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1743097, whole genome shotgun sequence.
ACCESSION   DASPGN010000549 DASPGN010000000
VERSION     DASPGN010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743097"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1764)
                     /locus_tag="VD788_15935"
     CDS             complement(<1..1764)
                     /locus_tag="VD788_15935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXV77802.1"
                     /translation="MNKFRLLVLLSLLATGAGAGPVVSAQEPSGVQERVTVTLVQVDA
                     LVIDKDGKTVPGLTKADFELILGGQPLEVATLDEACQIGATADPLPIEGDAPPAKVGD
                     GIKRRIVFAFDYYFLETTMRAQILDAARSMLEFGKTDDDEVMIVALAYGVRVEQRFTN
                     DLRQLVAALARMEHDVTLWARQFPLGASGEEYFDDLVTLMDVLEGYDGSKAVVLFSQA
                     RHIQTSMRDIFYNDVAMHALSARSAIYPANPDLLSGSGGGSDSLTRLANQSGGRMPFF
                     TNDVSLPYRQAQRDLSCRYTVGAYLDNTEGKKRKSVAVRVKNDDYRTRHPEMIKLFTD
                     EEKQENRSRAAFVDPEPYENPLVRADVYPVFPASRSTWDTLLMLTFPMPVAPGGADIE
                     VAATLMRGATRVDKAKRELHIDPPPGGGDVRPVTVFADTKLKDGDHRLTVVLTEPGTD
                     RLVTAEARFQVPPVFEDLLILRGPLFARTIPGGLFVRGDADKTADTKLDEILGEDKSF
                     EPLLVNQIATSDELLYYWNACVLGKLELNGQAVVKRTLMDDAGEIVHRLPELPLELEP
                     QARVKGLQCHSELETVAPNRIP"
     gene            complement(1853..>3730)
                     /locus_tag="VD788_15940"
     CDS             complement(1853..>3730)
                     /locus_tag="VD788_15940"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXV77803.1"
                     /translation="DYVGNRSARDESRFQTYAGRSCSTEPGCLLDITQNQSLRYIERD
                     IDSIQLHGRNQFPELAFGPFDGLELRWYGAHNTTSQDEPDVRFFANQTRTFDQDGDGA
                     ADQSVFQRLTSQGGNVSASQSSRRVFRTIDEASDVFSLSVESSFRGRGGRPGKLKLGA
                     FLDRTDREYLQDSFFYDFQQSQCCDARSPEVAENRGKGNDVEVDDPDALWTDIFTDDD
                     RIGIAPNAGTGPPNRPTVAPNQLLWVLQTLAEDINYDGEQSIDAWFAMAEVPLASHLD
                     LVLGVRREDTRMLVVPVSDRGDGKVPLIQAFCGTGPCENFPPDERPPVSGYGESNVSA
                     TRATADIDESFWLPAAGLIWEVAPRMKTRASWSRTIARPTFRELSPVGTEEFLAGDTF
                     VGNPDLVLSEIENYDLRWEWFPGPGDVLAASVFRKEITNPIEFQSFFLGSASVFQPLN
                     FETGELNGFEVEARTGLDVIWDGLRGLAVGANYTSLDSSVDVPEDVQELLAPFELDQE
                     TRRLQGQPEFLFNANVTFENPASETAFGVFYNRTGDTLLAGASKGESGVPNVFEIGRA
                     TLDFSISQGMGAGFQLTLKGRNLLEEDRETVYRLPDGREGVRTLQRSGRVIGMTLSWR
                     Y"
BASE COUNT          596 a         1245 c         1256 g          633 t
ORIGIN      
        1 cgggattcgg ttcggcgcca ccgtctcgag ctcggagtgg cactgcagcc ccttgacccg
       61 cgcttgcggc tcgagctcga gcggcagctc cggcagacga tgcacgatct ctccggcgtc
      121 gtccatcagc gtgcgtttca ccaccgcctg tccgttcagc tcgagcttgc cgagcacgca
      181 ggcgttccag taatacagca gctcgtccga cgtcgcgatc tggttgacga gcagcggctc
      241 gaagctcttg tcctcgccga ggatctcgtc gagcttggtg tccgcggtct tgtcggcgtc
      301 accccgcacg aacagccctc cgggaatcgt gcgcgcgaac agcgggccgc gcaggatcaa
      361 caggtcctcg aacaccggcg gtacttggaa ccgcgcctcg gccgtgacca accgatcggt
      421 tcccggctcg gtcaagacga ccgtcagcct gtgatccccg tccttcaact tcgtgtcggc
      481 aaataccgtg acgggacgga cgtcgccgcc gcccggcggc ggatcgatgt gcagctcgcg
      541 cttggctttg tccacgcggg tcgcgccgcg catcagcgtc gcggcgactt cgatatccgc
      601 gccgccgggc gcgaccggca tcggaaaggt gagcatcagc agcgtgtccc acgtcgagcg
      661 cgacgccgga aaaaccgggt agacgtcggc acgcacgagc gggttttcgt agggttccgg
      721 atcgacgaac gccgcgcggg atcggttctc ctgcttctcc tcgtcggtga acagcttgat
      781 catctcgggg tgacgcgtgc ggtagtcgtc gttcttgacg cggacggcga ccgacttgcg
      841 cttcttgccc tcggtgttgt cgaggtaggc gccgaccgtg tagcggcacg acaggtcgcg
      901 ctgggcctgg cggtagggca gcgacacgtc gttggtgaag aacggcatgc ggccccccga
      961 ctggttggcc aaccgagtca ggctgtccga cccgccgccg gagcccgaca gcaggtcggg
     1021 gttcgccggg tagatcgcgc tgcgcgccga cagggcgtgc atcgcgacgt cgttgtagaa
     1081 gatgtcgcgc atcgacgttt ggatgtggcg cgcctgcgag aacagcacga cggccttcga
     1141 gccgtcgtag ccctcgagaa cgtccatcag cgtgaccagg tcgtcgaagt actcctcgcc
     1201 gctcgcgccg agcgggaact gacgcgccca tagcgtgacg tcgtgctcca tccgtgccag
     1261 cgccgcgacc agttggcgca agtcgttggt gaagcgctgc tcgacgcgca cgccgtaggc
     1321 cagcgcgacg atcatcactt cgtcgtcatc cgttttgccg aactcgagca tcgatcgcgc
     1381 ggcatcgagg atctgggccc gcatcgtcgt ttcgaggaag tagtagtcga aggcgaagac
     1441 gatgcggcgc ttgatcccgt cgccgacctt cgccggcgga gcgtcgccct cgatcggcag
     1501 cgggtcggcg gtcgcaccga tctggcacgc ctcatcgagc gtcgccacct cgagcggctg
     1561 accgccgagg atcagctcga agtccgcctt ggtcaggccg ggcacggtct tgccgtcctt
     1621 gtcgatgacc agtgcatcga cctggaccag cgtgacggtg acccgctcct ggaccccgct
     1681 tggttcttgc gccgagacga ccgggccggc tcccgcgccg gtggcgagca acgacaagag
     1741 gacgagcagc cggaacttgt tcatgacgcc cctcaggccc gcaggacgaa cgcgggagat
     1801 gccggatgat agcagagcgc atggtcccgg cccggcccgg ctccgcagcg cgtcagtagc
     1861 gccaggagag cgtcatcccg atcacgcgtc cggagcgctg cagggtgcgg acgccctcgc
     1921 gtccgtcggg cagccggtaa accgtctcgc ggtcttcctc gaggaggttc ctgcccttga
     1981 gcgtcaactg gaagccggca cccatgccct ggctgatcga aaagtcgagt gtcgcccgac
     2041 cgatttcgaa gacgttggga acacccgatt cgcccttcga cgcaccggcg agcagcgtgt
     2101 cgccggtgcg gttgtagaag acgccgaatg cggtttcgct cgcggggttt tcgaacgtga
     2161 cgttggcgtt gaacaggaac tccggctgac cctgcaggcg gcgggtctcc tggtccagct
     2221 cgaacggcgc cagcagttcc tgcacatcct cgggaacgtc gacggacgag tcgagcgagg
     2281 tgtagttcgc gccgaccgcc aagccgcgaa gcccgtccca gatcacgtcg agcccggtgc
     2341 gggcctcgac ctcgaacccg ttcagctctc ccgtctcgaa gttcagcggc tggaagacgc
     2401 tggccgaacc gaggaagaag ctctggaact cgatcgggtt ggtgatctcc ttgcggaaca
     2461 cgctggccgc caggacgtcg ccgggaccgg gaaaccactc ccagcgcagg tcgtagttct
     2521 cgatctcgct cagcacgaga tcggggttgc cgacgaaggt gtcgccggcc aggaactcct
     2581 cggtgcccac cggcgacagc tcgcgaaagg tcggacgcgc gatcgtgcgg ctccacgacg
     2641 cgcgcgtttt catccgcggc gcgacctccc agatcaaccc cgcggccggg agccagaacg
     2701 actcgtcgat atccgcggtg gcacgagtcg cggaaacgtt cgactcgcca taacccgaga
     2761 cgggcggtcg ctcgtcgggc gggaagttct cgcacggccc cgtgccgcag aaggcctgga
     2821 tcaacggcac cttgccgtcg ccccgatcgc tcaccggcac gacgagcatc cgcgtgtcct
     2881 cgcggcgcac gccgaggacc agatcgagat gcgacgccag cgggacctcg gccatcgcga
     2941 accaggcatc gatgctctgc tcaccgtcgt agttgatgtc ctcggcgagc gtttgcaaca
     3001 cccacagcag ctggttcggc gccacggtcg ggcggttggg gggaccggtc ccggcgttcg
     3061 gcgcgatgcc gatgcggtcg tcgtcggtga agatgtcggt ccacagcgcg tccgggtcgt
     3121 cgacctcgac gtcgttgccc ttgccccggt tctcggcgac ctccggcgag cgggcatcgc
     3181 aacactgcga ctgctggaag tcgtagaaga aagaatcctg caggtattcg cggtcggtgc
     3241 ggtcgaggaa cgcgccgagc ttgagcttgc ccggtcgccc gccccgcccc cggaacgacg
     3301 attcgacgct gagcgagaag acgtcgctcg cctcgtcgat cgtgcggaag acgcgccggc
     3361 tgctctgcga cgccgagacg ttgccgccct gcgaggtgag tcgctggaag acggactggt
     3421 cggccgcccc gtcaccgtcc tgatcgaatg tccgtgtctg gttggcgaag aaacgtacgt
     3481 cgggctcgtc ctggctcgtc gtgttgtgcg cgccgtacca gcgcagctcg agcccgtcga
     3541 acgggccgaa cgccagctcc gggaactggt tgcggccgtg gagttggatc gagtcgatgt
     3601 cgcgctcgat gtagcgcagg ctctggtttt gcgtgatgtc caacaggcaa ccgggctcgg
     3661 tcgagcacga ccgcccggcg taggtctgga accgcgattc gtcccgcgcc gatcggttgc
     3721 cgacgtagtc
//
LOCUS       DASPGN010000550         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_805719, whole genome shotgun sequence.
ACCESSION   DASPGN010000550 DASPGN010000000
VERSION     DASPGN010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805719"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VD788_15945"
     CDS             complement(<1..362)
                     /locus_tag="VD788_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77804.1"
                     /translation="MRIEKLDTPADRLGHPLYAEHRADRRFFNPWSRFPHRLPSLLRF
                     WLRPKPRIAFRARPTALRLPRVARTGCAITAVGHCTFVIGCGDSTLVTDPHWGPRSKI
                     ERRKTPPGVAIDALPNDAV"
     gene            413..1552
                     /locus_tag="VD788_15950"
     CDS             413..1552
                     /locus_tag="VD788_15950"
                     /inference="COORDINATES: protein motif:HMM:NF017495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HXV77805.1"
                     /translation="METLISAERQWAIWTVLFAAAAFGLWSERMAWGRKLSSAVVTIG
                     LTFALSNLRLIPVAAPAYDAVWEYFVPLAIPLLLLRANLGRIVREAGPTLLAFAAGAA
                     GTVLGTVVAARLVPLGPEGYKLAGIFCATYIGGSLNYVAASEVLELRSGDLLTAGIAA
                     DNLVMTLYFLVLFALPSIPRLRRAYVTRHLEDVRETELGAGVDDASSPLRMLDVATAV
                     ALAASLCAVGYALAELAGMPSLAILIVTALTVALATAFPRRLSALAGGREIGTLLMMV
                     FFAVIGAGANVAVVLRQGPVLFVFAAVILAVHLAFILLAGRLMGLDLAEIVVASNANM
                     GGPTTAAAMASARRWTALVTPAILCGTLGYAVATFIGTAVGAALR"
     gene            complement(1559..2323)
                     /locus_tag="VD788_15955"
     CDS             complement(1559..2323)
                     /locus_tag="VD788_15955"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HXV77806.1"
                     /translation="MIREHLFSKRVSADPMFRWRGGEVSRIEGLSDAVFAFALTLLVV
                     SLEVPSTFDELSRTIRSFPAFAACFFLLVNVWYYHYKFFRRYGMEDFFTVSVNAVLLF
                     IVLFYVYPLKFVFSNLIDPLFGIDHSVVDADGTRSARVFAGHGRPLMLFFSAGFALIA
                     TLFIVMHVHAWRRRDELELDRLERFLTWAAIQAQLVSLTLALISIVLASIGGSWVPWS
                     GLIYFLMGPAHGINGYLSGVRAERMLDAMQRPTAPD"
     gene            complement(2453..>3486)
                     /locus_tag="VD788_15960"
     CDS             complement(2453..>3486)
                     /locus_tag="VD788_15960"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXV77807.1"
                     /translation="NAVYGMTGGQMAPTTLIGMETTTTPAGRVAENEGFPIKVCELLA
                     TLDAPYYLERVALGDAKHNHRARIAVRKAIRNQLDDRGFSLVELLSPCPTGWKLAPVE
                     AARFTIERMQRDFPLGVVRDGHGLSLGHPRLRRPVDLGDLPGLLETTDASTELELPDR
                     GRAIEETRHKFAGFGGQGVLLLGVLLARAGMLAGRNVSWIPSYGPEMRGGTANCGVTI
                     SDAEIGSPLIQNPDVLVAMNGPSLDRFAADVVPGGTIYYNSSMIAAAPARTDVVRVAV
                     PANDIADSLGNPKVANIVVLGSMLAGGAVVSREAILAALPGVIKDKPELIELNRRALH
                     AGMEAFAVV"
BASE COUNT          540 a         1191 c         1222 g          533 t
ORIGIN      
        1 accgcatcgt tcggcagcgc gtcgatcgcg acgccgggcg gcgtcttgcg ccgctcgatc
       61 ttcgagcgcg ggccccagtg cggatcggtg acgagcgtcg agtcgccgca gccgatgacg
      121 aacgtgcagt ggccgaccgc cgtgatcgcg cagcccgtcc tcgcgacccg tggcagccgc
      181 aaggcggtcg gccgcgcgcg gaaggcgatg cgtggtttgg gacgcagcca gaaccggagc
      241 aggctcggca gccggtgggg aaaccgcgac cacgggttga agaagcggcg atcggcacgg
      301 tgctcggcgt acagcggatg gccgagacgg tcggcggggg tatcgagctt ctcgatccgc
      361 acgagctgca gtatagtcca gcgctcgtgt gcgatcgtaa gtcggggacg cggtggaaac
      421 gctgatctcg gccgagcgcc aatgggcgat ttggacggtg ttgttcgccg cggcggcgtt
      481 cggcctgtgg tccgaacgga tggcctgggg ccgcaagctg tcgagcgcgg tcgtgacgat
      541 cggcctcacc ttcgcgctgt cgaacctgcg cctgatcccg gtcgcggcgc cggcgtacga
      601 tgcggtctgg gagtacttcg tgccgctggc gatcccgctg ctgctgctgc gggccaatct
      661 cggccggatc gtgcgtgagg cgggaccgac gttgctggcg ttcgcggcgg gcgcggccgg
      721 gacggtgctg ggtacggtcg tcgccgcgcg gctggtcccg ctcggccccg agggctacaa
      781 gctcgccggc atcttctgcg cgacctacat cggtggctcg ctgaactacg tcgccgcgtc
      841 cgaggtcctc gagctgcgat cgggcgacct gctgacggcc ggcattgccg cggacaacct
      901 ggtgatgacg ctgtacttcc tcgtgctgtt cgccttgccg tcgattccgc gcctgcggcg
      961 ggcgtatgtc acccgtcatc tggaagacgt gcgggagacg gagctcggcg ccggtgtcga
     1021 cgacgcgtcg agcccgctgc gcatgctcga cgtcgcgacc gcggtcgcgc tggccgcgtc
     1081 gctctgcgcc gtcgggtacg cgctggccga gctcgcgggt atgccgagcc tggcgatcct
     1141 gatcgtcacc gccttgaccg tcgcgctggc gacggcgttc ccccgccgcc tgtccgctct
     1201 cgcgggcggc cgggagatcg gcacgctctt gatgatggtc ttcttcgccg tgatcggtgc
     1261 cggtgccaac gttgccgtcg tgctgcgcca gggaccggtg ctgttcgtct tcgccgcggt
     1321 catcctcgcc gtgcacctgg cgttcatcct gctggccggc aggttgatgg gtctcgacct
     1381 ggccgaaatc gtcgtcgcct cgaacgccaa catgggcggg ccgacgaccg ccgccgcgat
     1441 ggcgagcgcc cggcggtgga ccgcgttggt cacgccggcc atcctctgcg ggacgctggg
     1501 ctacgccgtc gccaccttca tcggcaccgc ggtcggcgca gcactgcgat gaccgggctc
     1561 agtccggcgc ggtcgggcgc tgcattgcgt cgagcatacg ctcggcccgc acgcccgaga
     1621 ggtagccgtt gatgccgtgt gccggcccca tcaggaagta gatcagtccg gaccacggga
     1681 cccaactccc gccgatcgac gccaggacga tcgagatcag cgccagcgtc agcgagacca
     1741 gctgcgcctg gatcgcggcc caggtcagga agcgctcgag ccggtcgagc tccagctcgt
     1801 cgcgccggcg ccaggcgtga acgtgcatca cgatgaacag cgtcgcgatc agcgcgaatc
     1861 ccgccgaaaa gaagagcatc agcgggcgcc cgtgaccggc gaagacccgc gcgctccgcg
     1921 tgccgtccgc gtcgaccacc gagtggtcga tgccgaacag cggatcgatc aggttggaaa
     1981 aaacgaactt gagcggatag acgtagaaca gcacgatgaa cagcagcacc gcattgacgc
     2041 tgacggtgaa gaagtcctcc atgccgtagc gccggaagaa cttgtagtgg tagtaccaga
     2101 cgttgaccag caggaagaaa caggcggcga aggcgggaaa gctgcggatc gtccgtgaca
     2161 gctcgtcgaa ggtcgacggc acctcgagcg aaacgacgag cagcgtcaac gcgaaggcga
     2221 agaccgcgtc gctcagcccc tcgatccgcg agacttcgcc gccgcgccag cggaacatcg
     2281 gatcggccga gacgcgcttg ctgaacaggt gctcacggat cacgacccgg catgctagca
     2341 aacgggcgga gcaaacccgc acgaaaaacc ggccccgggt ccgcctcgtt gttggggggc
     2401 agccgggtgt tggaccccgg ggccggatcg ggccaggcgg gcccgaatca cctcagacga
     2461 cggcgaacgc ctccatgccg gcgtggagcg cacgtcggtt cagctcgatc agttcgggct
     2521 tgtccttgat caccccgggc agcgcggcga ggatcgcctc gcgcgagacg accgccccac
     2581 cggccagcat cgacccgagc acgacgatgt ttgccacctt gggattgccc agcgagtcgg
     2641 cgatgtcgtt ggccgggacg gccacccgga ccacatcggt ccgcgccggg gccgcggcga
     2701 tcatcgacga attgtagtag atcgtgccgc cgggcacgac gtccgcggcg aagcgatcga
     2761 ggctcggacc gttcatcgcc accagcacgt cggggttctg gatcagcggc gagccgatct
     2821 cggcatccga gatcgtcacg ccgcagttcg ccgtgccacc ccgcatctcc ggaccgtagg
     2881 acgggatcca cgacacgttg cgtccggcga gcatccccgc gcgggccagc agcacgccga
     2941 gcagcagcac cccctgtccg ccgaaaccgg cgaacttgtg gcgggtctcc tcgatcgccc
     3001 ggccgcgatc gggcaactcg agctccgtcg acgcgtcggt cgtctcgagc agaccgggca
     3061 ggtcgccgag atcgaccggc cgccgcagcc gcgggtggcc gagcgacagc ccgtggccgt
     3121 cccgtacgac accgagcggg aagtcccgct gcatccgctc gatcgtgaag cgcgccgcct
     3181 cgactggcgc gagcttccag ccggtcgggc acggcgacag cagctccacc agcgagaacc
     3241 cgcgatcgtc gagctggttg cggatcgcct tgcgcaccgc gatccgcgcc cgatggttgt
     3301 gtttggcgtc gcccagcgcg acgcgctcga gatagtacgg tgcgtcgagc gtcgccaaca
     3361 gctcgcagac cttgatcggg aacccctcgt tctcggcgac gcgccccgcc ggagtcgtag
     3421 tcgtttccat cccgatcagc gtcgtcggcg ccatctggcc gccggtcatg ccgtagaccg
     3481 cgttgt
//
LOCUS       DASPGN010000551         4535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_103282, whole genome shotgun sequence.
ACCESSION   DASPGN010000551 DASPGN010000000
VERSION     DASPGN010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103282"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2868
                     /locus_tag="VD788_15965"
     CDS             <1..2868
                     /locus_tag="VD788_15965"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HXV77808.1"
                     /translation="QYALVDNGGKHDRWELRAFQDWYLKLWLESVPGVAEVATIGGLE
                     KEYQVTVDPEALVARGIPLSRVVAAVRGGNREVGGRLLEFSGAEYMVRGRGYARSIEE
                     IESIVVDADGRGTPVLVRDVARVALGPAPRRGVADYNGLGDTVGGIVVMRHGENALRV
                     IERVEAKVEEVRSALPDGVEIVPVYDRSELIERAIDVLTHELILAMIIVSLVILIFLW
                     HVPSAIVPIVTIPVSVTLAFIPMWAMGITSNIMSLAGIAISIGVLVDGAIVEVENAYN
                     KIHRWQAAGSPGSFYAVRLAALQEVGPAVFFSLLVIAVSFLPIFTLVDQEGRLFKPLA
                     YTKNLTMAIAAVLAITLDPALRMVFARIEPLRFRPRWVAGVVSAIAVGRYRAEERHPI
                     SRVLIKLYEPICRLTLRFPVATILLALLLVASTVPVYLALGSEFMPPLDEGSLLYMPT
                     TLPGLSVDQAETLLQEMDRVIAEVPEVVSVYGKAGRADSATDPAPLSMVETVVMLEPR
                     DRWRRIDRFYSGWPGWLQAPFRAIWRDRLSREELVAELDRAMKYPGVTNAWTMPIKTR
                     IDMLSTGIRTPIGVKVLGPDLAEIERLGAEIEAVVRDLPGTRSVFAERTAGGYFLDFD
                     LRRDELARYGLAVDDAQSVILSAIGGEAVTRTIEGRERYSVNVRYPRELRSDVESLRR
                     VLVATPGGAQIPLGQIADIRLSTGPGMIRNENGLLAGYVYVDIVEDDIGGYVERAKTA
                     VDAAIERPTGYTIVWSGQYENMLRVRERLKIVIPVTLVLIAMILYMNTGSGVKTGIVL
                     LAVPFSLVGAVWLLWALDYNVSIAVWVGMIALMGLDAETGVFMLLFLDLACDDAVRDG
                     RMRTQADLREAIVHGAVKRIRPKMMTVVAALAGLMPVMLSVGTGSDMMKRVVAPMIGG
                     LVTSFVLELVVYPPIYETWKWRFVMQRGRALPAHPPGAAAS"
     gene            3000..3734
                     /locus_tag="VD788_15970"
     CDS             3000..3734
                     /locus_tag="VD788_15970"
                     /inference="COORDINATES: protein motif:HMM:NF039346.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77809.1"
                     /translation="MAAEAKVTEIGSDLYRLSVYAAEFDLQFNHFLVVDDEPLLFHTG
                     LKGMFPLLREAVASVLDPARLRWIAFSHFESDECGALNEWLALAPSAQPACSDLGAMV
                     SVNDFATRPARGLADGERLVTGKYRFRACRTPHLPHGWDAAVLFEETQRTLLCSDLFH
                     QLGDDAPLTTSDVVGRSIEALKSYQGGVLADYVPYTPYTGRLFDKLAELRPQRLAIMH
                     GSSFEGDGARALRDLAAGFAEVLGGR"
     gene            complement(3763..>4535)
                     /locus_tag="VD788_15975"
     CDS             complement(3763..>4535)
                     /locus_tag="VD788_15975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXV77810.1"
                     /translation="PTRLEARCVALYALRCYLPLSLVARGIRRLGRRARGAANRQEPV
                     NRLPEAGWRVLLPARAVRLAAPSRANGNVRLSELAVLAQAAAATEPNREIVEIGTFDG
                     RTTLNLAINSSSSCRVFTLDLPPEAPTRFPLDRRERKFVEKPAPGARFRGCDGAWEPA
                     AARITQLLGDSASYDWSAHEGKAGLVFVDGSHAYEYAKSDSEAAFRLVVPGGMVLWHD
                     YGVWPGVTRYLEELEERDGLGLRRIQGTSLIFWRRPSP"
BASE COUNT          661 a         1548 c         1592 g          734 t
ORIGIN      
        1 cagtacgcgc tggtcgacaa cggcggcaag cacgaccggt gggagctgcg cgccttccag
       61 gactggtacc tcaagctgtg gctcgagagc gtgcccggcg tggccgaggt ggccacgatc
      121 ggcggcctcg agaaggagta ccaggtcacg gtcgacccgg aggcgttggt cgcacgcggc
      181 attccgctgt cgcgggtggt cgccgcggtg cggggaggca accgggaggt cggcggccgg
      241 ctgctcgagt tctcgggagc ggaatacatg gtgcgcggcc gcggctacgc cagatcgatc
      301 gaggagatcg agtcgatcgt ggtcgacgcc gacggtcgcg gcacgccggt gctggtgcgc
      361 gacgtcgcgc gggtcgcgct cggcccggcc ccgcggcgcg gcgttgccga ctacaacggc
      421 ctcggtgaca cggtcggcgg catcgtcgtc atgcgccacg gcgagaacgc cctgcgcgtg
      481 atcgagcgcg tcgaggccaa ggtcgaggag gtgcgctccg cactgcccga cggcgtcgag
      541 atcgttccgg tctacgatcg ctcggagctg atcgagcgtg cgatcgacgt gctgacgcac
      601 gagctgatcc tggcgatgat catcgtcagc ctggtgattc tgatcttcct ctggcacgtg
      661 ccgtcggcga tcgtgccgat cgtgacgatc ccggtctcgg tgacgctggc cttcatcccg
      721 atgtgggcga tgggcatcac ctcgaacatc atgtcactcg cgggcatcgc gatctcgatc
      781 ggcgtgctgg tcgacggggc gatcgtcgag gtcgagaacg cctacaacaa gatccaccgc
      841 tggcaggccg cgggctcgcc cggcagcttc tacgccgtgc gtctcgcggc gctccaggag
      901 gtcggcccgg cggtcttctt ctcgctcttg gtcatcgccg tctcgttcct gccgatcttc
      961 accctggtcg accaggaagg acggctgttc aagccgctgg cctacaccaa gaacctgacg
     1021 atggcgatcg ccgcggtgct ggcgatcacg ctcgacccgg cactgcgcat ggtgttcgct
     1081 cgcatcgaac cgctccgctt ccgcccgcgg tgggtcgccg gggtcgtctc ggcgatcgcg
     1141 gtcggccgct accgggcgga ggagcgccac ccgatcagcc gcgtgctgat caagctctac
     1201 gaaccgatct gccggctgac gctgcgcttc cccgtggcga cgatcctcct cgcactgctg
     1261 ctcgtggcct cgaccgtgcc ggtttacctg gcgctcggct cggagttcat gccgccgctc
     1321 gacgagggca gcctgctcta catgccgacg acgctgcccg ggctctccgt cgaccaggcg
     1381 gagacgctgt tgcaggagat ggaccgcgtc atcgccgagg tgcccgaggt ggtcagcgtc
     1441 tacggcaagg ccggccgcgc cgacagcgca accgaccccg cgccgctgtc gatggtcgag
     1501 accgtggtga tgctcgaacc ccgcgaccgg tggcgccgca tcgaccgttt ctactccggc
     1561 tggccgggtt ggctgcaggc gccgtttcgc gcgatctggc gcgaccggct ctcgcgtgag
     1621 gagctggtgg cggagctgga ccgggcgatg aagtaccccg gcgtgaccaa cgcctggacg
     1681 atgccgatca agacccgcat cgacatgctg tcgaccggca tccgcacgcc gatcggggtc
     1741 aaggtcctgg ggccggacct ggcggagatc gagcgactcg gcgccgagat cgaggcggtc
     1801 gtgcgcgatc tgcccggcac gcgcagcgtc ttcgccgagc gcaccgcggg cggctacttc
     1861 ctcgacttcg atctgcgccg cgacgagctg gcacgctatg gcctggccgt cgacgatgcg
     1921 cagtcggtga tcctgagcgc gatcggcggc gaggcggtga cgcgcacgat cgaggggcgc
     1981 gagcgctact cggtcaacgt gcgctacccg cgggagctgc gcagcgacgt ggaatcgctg
     2041 cggcgcgtgc tggtcgcgac gccgggcggc gcgcagatcc cgctggggca gatcgcggac
     2101 atccggctgt cgaccgggcc ggggatgatc cgcaacgaga acggtctgct cgccggctac
     2161 gtctacgtcg acatcgtcga ggacgacatc ggcggctacg tcgagcgggc caagaccgcg
     2221 gtcgatgcgg cgatcgagcg cccgacgggc tatacgatcg tctggagcgg ccagtacgag
     2281 aacatgctgc gcgtccgcga gcggttgaag atcgtgatcc cggtgacgct ggtgctgatc
     2341 gccatgatcc tctacatgaa caccgggtcg ggcgtcaaga ccggcatcgt gctgctcgcc
     2401 gtaccgttct cgctcgtcgg cgcggtctgg ttgctctggg ctctcgacta caacgtctcg
     2461 atcgccgtct gggtcgggat gatcgcgctg atggggctcg acgccgagac gggcgtgttc
     2521 atgctgctgt tcctcgacct cgcctgcgac gacgcggtgc gcgacggccg catgcgcacg
     2581 caggccgacc tgcgcgaggc gatcgtccac ggtgcggtca agcgcatccg tcccaaaatg
     2641 atgaccgtcg tcgccgcgct cgccggcctg atgccggtga tgctgtcggt cggcaccggc
     2701 tcggacatga tgaagcgcgt cgtggcaccg atgatcggcg ggctcgtcac gagcttcgtg
     2761 ctcgagctcg tggtctaccc gccgatctac gagacctgga agtggcgctt cgtcatgcag
     2821 cgcggccgcg cgctgccggc gcacccgccc ggagcggcgg cgtcttgacc cgttccggtc
     2881 ggcgtggcgg tcgacgatcc acgggatcac cgcgcatgcc cgagcgatgc ggggcgcgct
     2941 cgcgtgttag actcgcgtga caaacggaca acgcgcgaga cgcggccaaa ggggaggaga
     3001 tggcagccga ggccaaggtc acggagatcg gatccgatct gtaccgcctg tcggtctacg
     3061 cggcggagtt cgatctgcag ttcaatcact tcctcgtcgt cgatgacgag ccgttgttgt
     3121 tccacaccgg tttgaagggg atgttcccgc tgctgcggga ggcggtggcg agcgtgctcg
     3181 acccggcgcg cctgcgctgg atcgccttca gccacttcga gtccgacgaa tgcggcgcgc
     3241 tcaacgagtg gctggcgctg gccccctcgg cccagcccgc ctgcagcgat ctcggtgcga
     3301 tggtcagcgt caacgacttc gcgacacgac cggcgcgtgg gctggccgac ggcgagcggc
     3361 tggtcaccgg caagtaccgc ttccgcgcct gccgcacgcc ccacctgccc catggctggg
     3421 acgcggcggt gctgttcgag gagacccagc ggaccctgtt gtgctccgac ctgttccacc
     3481 agctcggcga cgacgcgccg ctcaccacct ccgatgtggt cgggcgctcg atcgaggcgc
     3541 tgaagtcgta ccagggcggc gtgctcgcgg actacgtgcc gtacacgccg tacaccggcc
     3601 ggttgttcga caagctggcc gagttgagac cgcagcgact ggcgatcatg cacggctcga
     3661 gcttcgaggg cgacggcgcg cgcgcgctgc gcgacctcgc ggcgggcttc gcggaggttc
     3721 tcggtggacg ctgagtcgca ggccaacccg atcgccgcgg cgtcacggac tcggccgccg
     3781 ccagaagatc aggctcgtgc cttggatccg gcgtaacccg agcccatcgc gctcttcgag
     3841 ttcttccaga taccgcgtga cccccggcca caccccgtag tcgtgccaca gcaccatgcc
     3901 gccgggcacc acgaggcgga atgcggcttc gctatcgctc ttggcgtatt cgtaggcgtg
     3961 cgagccatcg acgaaaacga gccccgcctt gccctcgtgc gccgaccagt cgtaggacgc
     4021 cgagtccccc agcagctggg tgatgcgtgc ggcggcgggc tcccatgcgc cgtcgcagcc
     4081 gcgaaaccgc gcacccggag ccggcttttc gacgaacttg cgctcccggc ggtcgagcgg
     4141 gaaacgggtc ggcgcctccg gaggcaggtc gagcgtaaag acccggcacg aggatgacga
     4201 gttgatcgcc agattcagcg tggtgcgacc gtcgaacgtg ccgatctcga cgatctcccg
     4261 gttcggctcg gtcgccgcgg ccgcctgcgc cagcaccgcc agctcggaca ggcgcacgtt
     4321 gccgttcgcc ctcgacggtg ccgcgagccg taccgctcgg gccggcaaca gcacccgcca
     4381 accggcctcg ggtagacgat tcaccggttc ctgccgattc gccgcgcctc tcgcccgtcg
     4441 gcccaacctg cggatccccc tcgccacgag ggacagcggc agatagcaac gcagggcgta
     4501 cagggcgacg cagcgggcct cgagccgagt cggat
//
LOCUS       DASPGN010000552         4333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_923216, whole genome shotgun sequence.
ACCESSION   DASPGN010000552 DASPGN010000000
VERSION     DASPGN010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923216"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="VD788_15980"
     CDS             <1..1060
                     /locus_tag="VD788_15980"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXV77811.1"
                     /translation="RLDPRTSIVAIEPVAGDVHRAVRAALDAVGFREVLPRGGPVSLK
                     VNLGWDLFIPGSITSPLVVEALIRALSDHVGEIFIVEADQVLEDVERAYRDSGMAERC
                     ERLGARWVNMTREPTVTVEDRDNVILRRIDVPRILTRTSLVTVPVMKTHAKTWITGAL
                     KNQWGCLPTMRHEYHLVLDDALADLNHVVRPVLALMDGTVGLEGNGPKSGWPRVADRI
                     LCSTDPVALDTIQAICMGLDPARAEHIARCAARGIGICARDAIETRGLIPERSVVEFR
                     PPRHNAVSRVENLLRRSWLKRLVFDTPIFRVCLSGAKLYYRVWTARRAGAAWRTVVDH
                     PVYGAQWRAVLARRGGSG"
     gene            1057..2406
                     /locus_tag="VD788_15985"
     CDS             1057..2406
                     /locus_tag="VD788_15985"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXV77812.1"
                     /translation="MSRRSAVILGGGLAGMGAAWTLARSGWDDLLIVERGKELGGLAG
                     TFTRGEHFYPLAYHHILHRDRTLLYFLDRLGALERVRWRRIRMLFRLADRPYALGEPG
                     DFLRFPMTFGDKLRFARLMLRCSGKSDWTDWSTRSAAELVDAWAGPGVRRALFEPLTQ
                     LKFRLPAERVSGAWLGARLHFREGSAPLGYIPGTNWTKVLCDGLAERLARRGVRVELG
                     REVRALRATDGRVAALETDDGPRPTPGVVVSTVPTEVYRRLSPDDTSPGLDTIRYTAV
                     VSMICATRQSFGPDFYWMNLPTLDYNACGLFRLESLNPTIGAPGEACINFVTHLPHRD
                     DPFFERDDEAIVAGYRDDFRRLFGLELQPFWVELTRLPMYSPVFETGYVSPPVRSRRF
                     ANLYFAGNYRTFPSIASTGTALASGVEAGVRAVEDLGDSSPAGVEIQRFRLRSMPRP"
     gene            2447..3238
                     /locus_tag="VD788_15990"
     CDS             2447..3238
                     /locus_tag="VD788_15990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77813.1"
                     /translation="MLASMDGLAKNALLIVALLAVVIAGSYFYSRSIGPDRTATPMPA
                     TETPARPEILVGTPVHAQLKVGLDLRDQGRLDQAVAALEQVPADDAGYLVALESIGSI
                     RMRLGDWDGARRAFEELCTIQVTTRDLLANIAWVNFRLEDYDGAELAALRALEIEPDD
                     DELRYDVGLFRVARGELAEAIGTYERAILRNSDRETILHGLTGLSTLHELHPDIPGTH
                     YALAYFARRLSRDEMEVEELRHYLASNPAGPTADFARSRLDALGG"
     gene            3286..3975
                     /locus_tag="VD788_15995"
     CDS             3286..3975
                     /locus_tag="VD788_15995"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV77814.1"
                     /translation="MTAPIDTDKLIRWYDFQAPLYAWWRNRYDTVLVRYVTELIRRDP
                     PRELLDAGCGTGLFTIALARALPDRQLHGVDLSAGMLSVAARAGRERGLRNVHWLRAD
                     VRALPYPAESFDTVVAAGLLPNVNRRAAVLRELGRVLRPRGQLVLIEFDRSEMGAATR
                     AFFKLMILGYRAVSAVFRRFRFADGWSIERSTIDRDQLACWAAEVGFRIASAEPRGSH
                     VVYRLDRGSRA"
     gene            3972..>4333
                     /locus_tag="VD788_16000"
     CDS             3972..>4333
                     /locus_tag="VD788_16000"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXV77815.1"
                     /translation="MSKFALGNLFLLLSMVSAASSQVLLKVLLGEYHSSGSGVSGLRA
                     WLTADRALRGGASMVLLVVGFLCWLAALSRLELSYAYPIACSSILIVALLSAVFLGEA
                     VTPRMWIGTVLVLAGIAL"
BASE COUNT          586 a         1486 c         1509 g          752 t
ORIGIN      
        1 gcgcctcgac ccgcgaacga gcatcgtcgc gatcgaaccg gtcgcgggcg acgtgcatcg
       61 cgccgtccgc gctgcgctcg acgccgtcgg cttccgcgag gtgcttccgc gcggcggacc
      121 ggtgtcgctc aaggtcaacc tcggctggga cctgttcatc cccggctcga tcacctcgcc
      181 gctggtcgtc gaggcgctga tccgggcgct ctccgaccac gtcggcgaga tcttcatcgt
      241 cgaggccgac caggtgctgg aggacgtcga acgcgcctac cgcgacagcg gcatggccga
      301 gcgctgcgaa cgcctcgggg cgcgttgggt caacatgacg cgcgagccga cggtgacagt
      361 cgaggaccgg gacaacgtca tcctgcgccg catcgacgtg ccgcgcattc tgacgcgaac
      421 ctcgctcgtc acggtccccg tgatgaagac ccacgccaag acctggatca ccggcgcgct
      481 gaagaaccag tggggctgtc tgccgaccat gcgtcacgag taccacctgg tgctcgacga
      541 cgctctcgcc gatctgaacc acgtcgttcg cccggtgctt gcgctgatgg acggtacggt
      601 cgggctcgag gggaacgggc cgaagtccgg ttggccgcgc gtcgccgacc gcatcctgtg
      661 ctcgaccgat ccggtcgcgc tggacacgat tcaggcgatc tgcatgggcc tcgatccggc
      721 gcgagcggag catatcgcac gctgcgccgc gcgcggcatc ggcatctgtg cgcgcgatgc
      781 gatcgaaacg cgcgggctga tccccgagcg gagcgtggtc gagtttcgtc cgccgcgcca
      841 caacgccgtg tcgcgcgtcg agaacctgct gcgccggtcg tggctcaaac ggctggtctt
      901 cgacacgccg atcttccgcg tctgtttgtc gggggccaag ctgtactacc gcgtgtggac
      961 ggcgcgccgt gccggcgccg cctggcgcac cgtcgtcgat catccggtct acggcgcgca
     1021 gtggcgtgcc gtcctcgccc gacgcggggg ctccgggtga gtcgtcgctc cgcggtcatt
     1081 ctcggtggcg gactggccgg catgggggcg gcgtggacgt tggcgcgctc gggctgggac
     1141 gacctgctga tcgtcgagcg cggcaaggag ctgggcgggc tggcggggac gttcactcgc
     1201 ggcgagcact tctatccgct ggcctatcat cacatcctcc atcgcgatcg gacgctgctc
     1261 tactttctcg atcggctggg cgcgctcgag cgcgtgcgtt ggcgccggat ccgcatgctg
     1321 tttcgtctgg ccgacaggcc gtacgccctc ggcgaaccgg gcgacttcct ccgcttcccg
     1381 atgacgttcg gcgacaagct gcgcttcgcc cgactgatgc tccgctgctc cggcaagagc
     1441 gactggaccg attggagcac gcgcagcgcg gccgagttgg tcgacgcttg ggccgggccg
     1501 ggggtgcgcc gcgcgttgtt cgagccattg acccagctca agttccgcct gcccgccgag
     1561 cgggtcagcg gcgcgtggct cggagcccgg ctgcatttcc gcgagggctc ggccccgctc
     1621 ggctacatcc ccggcaccaa ctggacgaag gtgctgtgcg acggtctcgc cgagcggctc
     1681 gcgcgcaggg gcgtacgggt cgagctcggc cgggaggtgc gggcactgcg ggcgaccgac
     1741 gggcgcgtcg ccgcgctcga gaccgatgac ggaccgcgac cgacgccggg ggtggtggtc
     1801 agcaccgtcc cgacggaggt ctatcgccgg ctgtcgccgg acgacacgag ccccggtctc
     1861 gacacgatcc gctataccgc cgtcgtctcg atgatctgcg cgacgcggca gagcttcggc
     1921 cccgatttct attggatgaa cttaccgacc ctcgattaca acgcctgcgg cctgttccgc
     1981 ctcgagtcgc tgaacccgac gatcggtgca cccggcgagg cctgcatcaa cttcgtcacg
     2041 catctgccgc atcgcgacga cccgttcttc gagcgcgacg acgaggcgat cgtcgccggc
     2101 tatcgcgacg acttccgccg gttgttcggt ctcgagctgc agccgttctg ggtcgagctg
     2161 acgcggctgc cgatgtactc gccggtcttc gagacgggct acgtgagtcc cccggtgcgg
     2221 agccgtcgct tcgccaacct gtactttgcc ggcaactacc gcaccttccc gtcgatcgcc
     2281 tcgaccggta ccgcgctcgc ctccggtgtc gaggccggcg tccgcgcggt cgaggatctc
     2341 ggcgacagca gcccggccgg cgtcgagatc caacggttcc ggctgcgctc gatgccacga
     2401 ccgtagagcg tatccgccgt gcttgacgct cacccggtga gattctatgc tcgcgtcgat
     2461 ggacggactg gcgaagaacg cattgttgat cgtggcgctg ctggccgtcg tcatcgccgg
     2521 ctcctacttc tactcacgct cgatcggacc ggaccggacc gcgacgccga tgccggcgac
     2581 cgagacgccc gcgcgacccg agatcctggt cggcactccg gtccacgcgc agctcaaagt
     2641 cggcttggac ctgagggacc agggtcggct cgaccaggca gtcgccgcgc tcgagcaggt
     2701 gccggcggac gatgcgggct atctggtcgc gctcgagagc atcgggtcga tccgaatgcg
     2761 gctcggcgac tgggacggcg cgaggcgggc gttcgaggag ctgtgtacga tccaggtcac
     2821 gacacgtgac ctgctggcca acatcgcctg ggtcaacttc cgcctggagg actacgacgg
     2881 ggccgagctg gcagcgctgc gcgcgctcga gatcgagccg gacgatgacg agctgcgcta
     2941 cgacgtggga ctgttccgcg tggcccgggg cgaattggcc gaagcgatcg ggacctacga
     3001 gcgggcgatc ctgcgcaact ccgaccgcga gacgatcttg cacggtctca ccgggctgtc
     3061 gacgctccac gaactccatc ccgatatccc cggcacgcat tacgcgctgg cctacttcgc
     3121 ccggcggttg tcccgcgacg agatggaggt cgaggaactg cggcattacc tggcgtcgaa
     3181 cccggccgga ccgaccgccg acttcgctcg ctcgcgcttg gacgcactgg gcggctagcg
     3241 ttccgcccgg cgacgggcag gccgattgcc gccgcgccgt ttcgaatgac cgctccgatc
     3301 gacaccgaca agctgatccg gtggtacgat ttccaggccc cgctctacgc ttggtggcgc
     3361 aatcgctatg acacggtgct ggtgaggtac gtgacggaac tgattcgacg tgacccgccg
     3421 cgcgagctgc tcgacgcagg gtgcggcacc ggcctgttca cgatcgccct ggcgcgcgcg
     3481 ctgcccgacc gccagctgca tggtgtcgac ctgtcggccg gcatgttgtc ggtcgcggcg
     3541 cgcgcgggac gcgagcgcgg tctgcgcaac gttcactggc tgcgagccga cgtgcgggcg
     3601 ctcccgtacc cggcggagtc gttcgacacc gtcgtcgcgg cgggactgct gccgaacgtc
     3661 aatcgacgcg ccgcggtgct gcgcgagctg ggccgcgtgc tgcgcccgcg gggccaactc
     3721 gtgctgatcg agttcgaccg gagcgaaatg ggcgctgcga cgcgcgcatt cttcaagctg
     3781 atgatcctcg gctatcgggc ggtgtcggcg gtcttccgcc gtttccgctt cgccgacggc
     3841 tggtcgatcg agcgcagcac gatcgaccgg gaccagctgg cgtgctgggc cgccgaggtc
     3901 ggctttcgca tcgcctccgc cgagccccgg ggctcccacg tggtctatcg gctcgatcgg
     3961 ggatcgcgag catgagcaag ttcgccctgg gcaacctgtt cctgctgctg tcgatggtca
     4021 gcgccgcgag cagtcaggtc ctgctgaagg tcttgctggg cgagtaccac tcctccggtt
     4081 ccggcgtgag cggtctgcgc gcgtggctga cggccgaccg cgcgctgcgc ggtggcgcgt
     4141 cgatggtgct gctggtggtc ggcttcctct gctggttggc ggctctttcg cgactcgagc
     4201 tgagctacgc ctatccgatc gcgtgctctt cgatcctgat cgtcgcgctg ttgagcgcgg
     4261 tctttctcgg cgaggcggtc acgccgcgga tgtggatcgg gacggtgctg gtgctcgccg
     4321 ggatcgcact gtt
//
LOCUS       DASPGN010000553         2414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1390310, whole genome shotgun sequence.
ACCESSION   DASPGN010000553 DASPGN010000000
VERSION     DASPGN010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1390310"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VD788_16005"
     CDS             complement(<1..1008)
                     /locus_tag="VD788_16005"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXV77816.1"
                     /translation="MEPPWAVVVDRNPASRTLMSEVLEQHGFSVRRVDDPWQTLDTPA
                     TRSASLVSLAFESTQDRLDSLIERLAQLEPRPLIVGVGDPGSTLSTELASELYDLVPA
                     PIGRAALERMVRRCKTRLALVKRLERLQEELKHRHGFSGFVGRGDAMRRVRSELERLS
                     VTDDPVWISGEPGTGKESAARQLHATSPRRESEFVPIDCTELSRVADVLPTSGEFPRG
                     EGGTLYLAELPALRPDLQEELLRTVGMLRARGIRAPRGVRLVVGSRLDPTRAGSEGRL
                     LPELQRQLSTHAIAIPPLRERREEIVLLADHFLSQICEINRLRRIHLGHETIARLESY
                     PW"
     gene            complement(1109..>2414)
                     /locus_tag="VD788_16010"
     CDS             complement(1109..>2414)
                     /locus_tag="VD788_16010"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77817.1"
                     /translation="PAVPLNRADEEYVSGRMTQAEILEKYGLHQQALEQVREVTAKFP
                     GHVPAQEKLVLLEREGGTAEGLSRALVAVALARRAAGDADGARQACAEASRIGPLEPA
                     TLTMLAQLGLIDRARPTAARAAVVPPAPSREPRPAPAPAAFRPAAAGRDDTLFIDFDA
                     MDDEDAATPAEPSPKHTTAPPPPGVTTPPAAADQPDPASEVVAEVERLLAVGETAEAE
                     RRLVALETLGWAADAARSLRQRIDAAKASMISRSSLVDSDLADDDDLSAITAALESEL
                     FADEMTALDAEPESEQSLEEVFAAFKQHVDREVGSEDYRTHYELGIGYKEMGLMDEAI
                     AAFEQAVRSPEFGRDAYAMLAVCHRERDDLEAAARCYREAIARPGSDPETVKHLRYDL
                     ADVLLIAGDRAGALVQLRGVLGADPTFRDVRQRVAELESLAS"
BASE COUNT          356 a          878 c          808 g          372 t
ORIGIN      
        1 ccatggatag ctctcgagcc gcgcgatcgt ctcgtgaccg aggtggatcc gtcgcaagcg
       61 attgatctca cagatctgcg agaggaagtg atcggccagc agcacgatct cctcgcgacg
      121 ctcgcgcaac ggcgggatcg cgatcgcgtg cgtggacagc tgacgctgca actcgggcag
      181 cagccgtccc tcgctgccgg cgcgggtcgg atcgagtcgt gagccgacga ccagacggac
      241 cccgcggggc gcgcggattc ctcgcgcacg gagcatcccg accgttcgca gcagttcctc
      301 ctggaggtcc ggccgcagcg cgggtagctc cgccaagtag agtgtgcccc cctcgccgcg
      361 gggaaactcc ccgctcgtcg gcagaacgtc cgcgacccgg ctcagctccg tgcagtcgat
      421 cgggacgaac tccgactcgc gacgcgggga ggtcgcgtgc aactgccgcg cggccgactc
      481 cttgccggtg ccgggctcgc cgctgatcca gactggatcg tcggtgaccg acagccgctc
      541 gagctcggac cgcactcgtc gcatcgcatc gccgcgaccg acgaatccgc tgaagccatg
      601 ccggtgcttg agctcctcct gcaagcgctc caagcgcttc accagtgcca gtcgcgtctt
      661 gcaccggcgc accatccgct cgagcgccgc ccgaccgatc ggcgccggaa cgagatcgta
      721 cagctcgctc gcgagctccg tgctcaacgt cgaccccggg tcgccgacgc cgacgatcaa
      781 cggccgcggt tcgagctgcg ccaaccgctc gatcaacgaa tcgagccgat cctgcgtcga
      841 ttcgaaggcg agcgaaacga gtgacgccga gcgggtcgcc ggagtatcca gggtctgcca
      901 cgggtcgtcg acgcgccgaa ccgagaagcc gtgctgttcg agcacctcgc tcatcaacgt
      961 tcgcgacgcc ggattcctgt cgacgaccac ggcccacggt ggttccatct agcgactcct
     1021 tctgcacgca gcgacgatcg atgctctggc tgcgcaggag tataccgatc ccggatggaa
     1081 gaaggaatcc tgtccgtcgt gcccggtgtc aggaggcgag ggactcgagc tcggcgactc
     1141 gctggcgtac gtcgcgaaag gtcggatcgg cccccagcac gccacgcaac tgaacgagcg
     1201 ccccggcccg gtcgccggcg atcaacagca cgtcggccag atcgtaacgg aggtgcttga
     1261 ccgtctccgg atccgagccc gggcgcgcga tcgcttcgcg gtagcatcgc gcggctgcct
     1321 ccaaatcgtc gcgctcccga tggcagaccg cgagcatcgc gtacgcgtca cggccaaact
     1381 ccggggagcg caccgcttgc tcgaacgccg cgatcgcctc gtccatcaat cccatctctt
     1441 tgtagccgat gccgagctcg tagtgcgtcc gataatcctc gctgcccacc tcgcggtcga
     1501 cgtgctgctt gaacgcagca aaaacctcct cgaggctctg ctcggactcg ggctcggcgt
     1561 cgagcgccgt catctcgtcg gcgaacaact cgctctcgag tgccgccgtg atcgcgctca
     1621 ggtcgtcgtc gtcggccaga tcgctgtcga ccaggctcga acgcgagatc atcgacgcct
     1681 tcgccgcgtc gatccgctgg cgcaggctcc gagcggcgtc ggccgcccaa ccgagcgttt
     1741 cgagagcgac cagccgtcgt tcggcctccg cggtctcgcc gaccgccagc agccgttcga
     1801 cttcggcgac gacctcgctg gccgggtccg gctggtccgc ggcggcgggg ggagtcgtaa
     1861 cgccgggcgg gggcggtgct gtggtgtgct tcgggctcgg ttcggccggc gtcgccgcgt
     1921 cctcgtcgtc catcgcgtcg aaatcgatga acagcgtgtc gtcccgaccg gccgcggcgg
     1981 ggcggaaggc ggccggcgcg ggagcgggac ggggctctcg gctcggtgcg gggggaacga
     2041 ccgccgctcg cgccgcggtg ggacgtgccc ggtcgatcag cccgagctgg gccagcatcg
     2101 tcagcgtcgc cggctcgagc ggtccgatcc gcgaggcttc ggcgcaggcc tgacgggcac
     2161 cgtcggcgtc gccggcggcc cggcgcgcca gcgccaccgc gaccaacgcc cgcgacaggc
     2221 cctccgccgt cccgccctcg cgctcgagca gtacgagctt ctcctgggcc ggcacgtgac
     2281 cggggaactt tgccgtgact tcgcgcacct gctcgagcgc ctgctggtgc aggccgtact
     2341 tctccaggat ctcggcctgc gtcatccgcc cgctgacgta ctcctcgtcg gcccggttca
     2401 gcgggaccgc cggc
//
LOCUS       DASPGN010000554         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1039688, whole genome shotgun sequence.
ACCESSION   DASPGN010000554 DASPGN010000000
VERSION     DASPGN010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039688"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1332
                     /locus_tag="VD788_16015"
     CDS             220..1332
                     /locus_tag="VD788_16015"
                     /inference="COORDINATES: protein motif:HMM:NF004996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXV77818.1"
                     /translation="MNKTPQRDEPTTDQAESILLDAAETSPAAAAIDFAAVRRICSDA
                     SRPLEQRLEAFEDVHESEVGDTILSRARNVEREVGLRQIYLKFEGDNPSGTQKDRIAF
                     AQAMDALRRGYDSITVATCGNYGVAVALAAEVAGLRCIVYVPESYHTRRVREIAQRGA
                     EVLRVPGDYESVVEISRDRARDDEIYDANPGGANTALQLRAYGEIAYEIYDELRDAPA
                     AVAVPVSNGTTLAGIHKGFVSLYRRGKTSRVPRMVAGSSFGKNPIVQAFRKNAPACED
                     LRPERIRETSVNEPLINWSSFDGDHALQSIRESQGWASYVSDRSMLRFSRIVREQQGL
                     SVLPAATAGLIALLENHQSQPLINDRYVAILTGRRS"
     gene            1329..>2169
                     /locus_tag="VD788_16020"
     CDS             1329..>2169
                     /locus_tag="VD788_16020"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HXV77819.1"
                     /translation="MSEAPFEGRAVVYCDGAFGTTNGKTAHGLVRRSRRYAVGSVIDS
                     RHAGRDAGELLDGKPAGIPIVATLEAALGRDRPTHFVIGLAPDGGRLDPAARSAVCRA
                     IDAGLNVDSGLHDFLCEDDEIAALARRRGVRLRDVRKPPPRAELHFFDGRKIGQVEAL
                     RVAVLGTDSAVGKRTTAWILVDALTDAGHPAEMIGTGQTAWLQGSRYGIVLDSLVNDF
                     ISGEIEHAIWSCWTERRPRVIVLEGQGSLMNPAYPGGMELLAAGRPHVVVLQHAPRRT
                     HYDG"
BASE COUNT          349 a          748 c          759 g          313 t
ORIGIN      
        1 atctgggacg gggtcgacct gcaccccacc gtcagcttcg cctaccgccg tcacgtcttc
       61 accacgctgt gggtggcgct cgaccacccc ggcgccgcct acagcatcgt cttctgaccc
      121 cggaagcggc ttctcccgcc caactgtcga tccgcggaca gttgtgctta caatgcggta
      181 tggctccgca acgacacacg agggagtgaa cgccgcgcga tgaacaagac accccagcga
      241 gacgaaccga cgaccgatca agccgagtcg atcctgctcg acgcggcgga aacctcaccg
      301 gcggcggccg cgatcgattt cgccgcggtt cgccggatct gctccgatgc ctcgcggccg
      361 ctcgaacagc ggctcgaggc gttcgaggac gttcacgaat ccgaggtggg cgatacgatc
      421 ctctctcgcg cgcgcaacgt cgagcgcgag gtcggcctgc gtcagatcta cctcaagttc
      481 gaaggcgaca atccgtcggg cacgcagaag gaccggatcg cgttcgcgca ggcgatggac
      541 gcgctccggc gcggttacga ctcgatcacg gtcgcgacct gcgggaacta cggggtggcg
      601 gtcgcgctcg ccgccgaggt cgccggcctg cgctgcatcg tctacgtgcc ggagagctac
      661 cacacgcggc gggttcgcga gatcgcgcaa cgcggcgccg aggtgctgcg cgtacccggc
      721 gactacgaga gcgtcgttga aatctcgcgg gaccgcgccc gcgacgacga gatctacgac
      781 gccaaccccg gcggggcgaa tacggcgctg cagctgcggg cctacggcga gatcgcctac
      841 gagatctacg acgagctacg cgacgcgccg gccgcggtgg ccgtaccggt gtcgaacggg
      901 acgaccctgg ccgggatcca caaggggttc gtcagtctct accgccgcgg caagacgtcg
      961 cgcgtcccgc gcatggtggc cgggtcgtcg ttcggcaaga atccgatcgt ccaggctttc
     1021 cgcaagaacg ccccggcctg cgaggatctg cgcccggagc gcatccgcga gacgtcggtc
     1081 aacgagccgt tgatcaactg gtcgtcgttc gacggcgatc acgcgctgca gagcatccgc
     1141 gaatcgcagg gctgggcatc ctacgtcagc gaccgcagca tgctgcgctt ctcgaggatc
     1201 gttcgcgagc agcagggctt gagcgtcctg ccggcggcga ccgccgggct gatcgcgttg
     1261 ctcgagaacc accagtcgca gccgctgatc aacgaccgtt acgtcgcgat cctgaccggg
     1321 cgcaggtcat gagcgaggcg ccgttcgagg gccgggccgt cgtctactgc gacggggcgt
     1381 tcggcacgac caacggcaag accgctcacg gcttggtacg gcggagccgg cgctacgccg
     1441 tcggctcggt catcgactcg cgccacgccg gtcgggacgc gggcgagctg ctcgacggca
     1501 agccggcggg gatcccgatc gtggcgacgc tcgaggccgc gctcggccgc gacaggccga
     1561 cgcatttcgt catcggtttg gcaccggacg gcgggcggct cgaccccgcg gcgcgatcgg
     1621 cggtctgccg ggcgatcgac gccgggctga acgtcgactc ggggctgcac gatttcctgt
     1681 gcgaggacga cgagatcgcg gcgctggcac gccgccgcgg cgtcaggctg cgcgacgtgc
     1741 gcaaaccgcc gccgcgggcc gagctgcact tcttcgacgg caggaagatc ggccaggtcg
     1801 aggcgctgcg cgtcgcggtg ctcggcaccg actcggcagt cggcaagcgg acgaccgcct
     1861 ggatcctggt cgacgcgctg accgacgccg gtcacccggc cgaaatgatc ggcaccggac
     1921 agacggcgtg gctgcagggc tcgcgctacg ggatcgtgct cgactcgctg gtcaacgact
     1981 tcatcagcgg cgagatcgag cacgcgatct ggagctgttg gaccgagcga cgtccgcggg
     2041 tgatcgtgct cgaggggcag ggcagcctga tgaacccggc ctaccccggc ggcatggagc
     2101 tgttggccgc cggcaggccg cacgtcgtcg tcctgcagca cgcgcctcga cggacgcact
     2161 acgacggct
//
LOCUS       DASPGN010000555         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_924104, whole genome shotgun sequence.
ACCESSION   DASPGN010000555 DASPGN010000000
VERSION     DASPGN010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924104"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VD788_16025"
     CDS             <1..476
                     /locus_tag="VD788_16025"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV77820.1"
                     /translation="ALGSSGDDAAALEAFERSIEIEPRAPQTWQAYATALGNLGRFDE
                     AAAAWRRCVELAPGEVGVLWLRLGQAELRAGRTIPAHEALERAAETLTGRPELDAARA
                     EAMLRLASVRLGQGEPTVALALVRRADAIGQLAPGLLLRAGGIAWQAGDEATGRV"
     gene            835..1920
                     /locus_tag="VD788_16030"
     CDS             835..1920
                     /locus_tag="VD788_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77821.1"
                     /translation="MSADREQARPVRRVAHTRPLYFHDGKLYCARFDRVYSTTDYGRT
                     FNEEGELSVRIPLRQLIKRFRLAQRVMRAAIYRMRVLPDGTTVYTFRGGVYVRRPGEP
                     TARRTYAVEKGSRPVSLAAGADGTVVFGEYWSNQARDAVRIFGSRDSGASWEVLHTFR
                     PGEIRHVHGISYDRWEDCFWVCTGDEGDENRLLRASKHFSELRIIRQGGQGNRFYSLI
                     VREHDLVTATDTPLEDNFICTIDKRTAELKHVARIENASFYSCTVGDRTFVSTNAEPS
                     AVNDTRTSYVWSGSRDDDWRRVLTFPADLYHRLTELPGVPQGLFQYPRIFFPEGENPG
                     RTLVCHAIGVRGYDGAMLCFDTEAWAR"
     gene            1925..2866
                     /locus_tag="VD788_16035"
     CDS             1925..2866
                     /locus_tag="VD788_16035"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HXV77822.1"
                     /translation="MNLPATRVRVPATVEFLARCVHGARRRFGRSRGGVDDDRSSRAP
                     VRNDARVFARALAELGIRSGDLLMVHSSGAAIERLGWELPEFLDFLLDYLGPAGTLAM
                     PSHPKLLRRNGRDLYDVRRSPSTVGLLTELFRRRPGTLRSRYPLSAAAARGVRAEALI
                     TDHRRSWAPHDEHSPYAKLADLGGKALCIGCPLDRITVLHVAEDVLRDELPVAGFHER
                     REIGMRDGGPEEQMTVHARAGWLWWYLALGRWTYDMYSNGLARDARIDGVTLRVTDAG
                     ETVEWMKTGLRGGRWIYPLAWANRWLRLGPPGQEHDA"
     gene            2863..>3712
                     /locus_tag="VD788_16040"
     CDS             2863..>3712
                     /locus_tag="VD788_16040"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXV77823.1"
                     /translation="MKNRDDAAAAATDATREQILRSIARIRAFEMRLLELFSDGKLFG
                     TTHTSVGQEACAAGLYTQIDPRRDAIFTNHRCHGHYLAYGGSMESLMAEIMGKQGATC
                     LGRGGSQHLCSGRFFSQGVQGGSMPIAAGYAARVKECGEGGIVVAHIGDGTLGEGVVY
                     ETANIVSLRGLPLLVVLENNGVAQSTDTKTTTAGDIETRFAAFGIDVDRRSDADPVGL
                     ADHFAEVVERVRAGRPFVQVVDTFRLMAHSKGDDDRDRAAIDEAWRNDYFARLLDSGD
                     PEASAAW"
BASE COUNT          544 a         1255 c         1337 g          576 t
ORIGIN      
        1 tggccctcgg gtcgtccgga gacgatgccg cggcgctcga ggccttcgag cgttcgatcg
       61 agatcgaacc gcgagcgcct cagacgtggc aggcctacgc caccgcgctg ggcaatctgg
      121 gacgtttcga cgaggcggcc gcggcctggc ggcgctgcgt cgagctcgcg ccgggggagg
      181 tgggcgtcct gtggttgcgg ctcggacagg cggagctgcg cgcggggcgg acgatccccg
      241 cccatgaggc gctcgagcgt gccgccgaga ccctgaccgg ccggcccgag ctggacgcgg
      301 cccgtgccga agcgatgctt cgtctggcgt cggtgcgcct cgggcaaggc gaaccgaccg
      361 tggcgctcgc gctcgtccgc cgcgccgacg cgatcgggca gctcgcgccg ggtctcctgc
      421 tgcgcgccgg cgggatcgct tggcaggccg gcgacgaggc gaccggacgc gtgtgagtcg
      481 aacaaagcct ggcccgcgat gtcggagcgg ctcgccgcga gtacgatcgc gcgctcgcgc
      541 tcgagcgcgc cggtaggctc gatgaggcgc tggtcgccca tcgcacgctg ctcctcgtcc
      601 ggccggacca tcctccgacc ctgttcaatc tcggacgcgt gctgctcgcg gccggacgcc
      661 cggcagaggc tgccgacata ctgcgccggg gtctggcctc gaccgacgac gaacgcgcgc
      721 gcgcgttgct cgaacgcgcc acggccgcag agcggtagcg cacgcggcga ccgtcgtcgc
      781 cgcggccgtc gcgccgcctt gacacccgga ccgcggaggt cgtactttcg gcccgtgagc
      841 gccgatcgcg aacaagcgcg cccggtccgg cgggtcgccc atacacggcc actctatttt
      901 cacgacggaa agctgtactg cgcgcgcttc gaccgcgtgt actcgacgac cgactacggt
      961 cgcacgttca acgaggaggg cgagctgtcg gtgcgaatcc cgctgcgaca gctgatcaag
     1021 cgctttcgtc tcgcgcagcg cgtgatgcgc gccgcgatct accgcatgcg ggtgttgccc
     1081 gacggcacga cggtctacac cttccgcggc ggggtgtacg tgcgccggcc cggcgagccg
     1141 accgcacggc gaacctacgc cgtggagaag ggtagccgtc cggtctcgct cgcggcgggc
     1201 gccgacggta cggtcgtgtt cggcgagtac tggtcgaacc aggcccgcga cgcggtgcgg
     1261 atcttcggca gtcgagactc cggcgcgagc tgggaggtgc tgcacacctt ccgtcctggg
     1321 gagattcggc acgtccacgg gatcagctac gaccggtggg aggactgctt ctgggtctgc
     1381 accggggacg agggtgacga gaaccgccta cttcgcgcgt ccaagcactt ctccgagctg
     1441 cggatcatcc ggcagggcgg acagggcaat cggttctatt cgctgatcgt ccgcgagcac
     1501 gacctggtca cggcgaccga tacgccgctc gaggacaatt tcatctgcac gatcgacaag
     1561 cggaccgccg agctgaagca cgtggcacgg atcgagaacg cgagcttcta cagctgcacg
     1621 gtcggggatc gaacattcgt ttcgacgaac gcggagccga gcgcggtcaa cgatacgcgt
     1681 acttcgtacg tctggtcggg aagccgcgac gacgactggc gccgcgtact gaccttcccc
     1741 gccgacctgt accatcggtt gaccgagttg cccggcgtgc cgcaggggct gttccagtac
     1801 ccgcggatct tcttcccgga gggagagaat ccgggccgga cgctggtctg ccacgcgatc
     1861 ggcgttcgcg gttacgatgg cgcgatgctc tgtttcgata ccgaggcatg ggcccgctga
     1921 gccgatgaat ctccctgcga cgcgtgtccg cgttccggcg acggtcgagt tcctcgcgcg
     1981 ctgcgtgcac ggagcgcgac gccggttcgg acgctcgcgt ggcggcgtgg acgacgaccg
     2041 atcgtcacgc gcgcccgtcc gcaacgacgc gcgggtcttc gcccgcgcgc tcgcggagct
     2101 ggggatccgt agcggcgacc tgctgatggt ccactcgagc ggcgcggcga tcgagcggct
     2161 gggttgggag ctgccggagt ttctcgactt cctgctcgac tacctcggcc cggccggcac
     2221 gctggccatg ccgagccacc ccaagctgtt gcgccggaac ggacgcgacc tgtacgacgt
     2281 gcgtcgcagc ccgtcgacgg tggggctgct gacggagttg tttcggcgcc gtcccggcac
     2341 gctgcgcagc cgttaccccc tttccgccgc cgccgcccgg ggtgtgcgag cggaagcgtt
     2401 gatcaccgat caccggcgat cgtgggcgcc ccacgacgag cactcgccgt atgccaagct
     2461 ggccgatctc ggaggaaaag cgctgtgtat cggctgcccg ctcgatcgga tcacggtgct
     2521 tcacgtcgcc gaggacgtgc tgcgcgacga gctaccggtt gcgggattcc acgaacgacg
     2581 cgagattggg atgcgggacg gggggccgga agagcagatg acggtgcacg cccgagcggg
     2641 gtggctgtgg tggtacctgg ccctcggtcg ctggacctat gatatgtact ccaacggctt
     2701 ggcacgggat gcccgaatcg acggcgtgac gctgcgggtg accgacgcgg gggagaccgt
     2761 ggagtggatg aagaccgggc ttcgcggcgg gcgctggatc tacccgctcg cctgggccaa
     2821 ccgctggctg cgtctcggcc cgcccggtca ggagcacgac gcgtgaagaa ccgcgacgac
     2881 gcggcggcgg cggccaccga cgcgacccgg gagcagatcc tgcgctcgat cgcacggatc
     2941 cgcgcgttcg agatgcggct gctcgagctg ttctccgacg gcaagctgtt cggcacgacc
     3001 cacaccagcg ttggccagga ggcttgcgcc gcgggcctgt acacccagat cgatccgcgg
     3061 cgcgatgcga tcttcaccaa ccaccgttgc catggtcact acttggccta cggcggatcg
     3121 atggagtcgt tgatggccga gatcatgggc aagcaggggg cgacctgcct cggtcgcggc
     3181 ggatcccagc acctgtgctc cggccggttc ttctcgcagg gcgtgcaggg tggctcgatg
     3241 ccgatcgccg ccggctacgc ggcccgggtc aaggagtgcg gcgagggcgg gatcgtcgtg
     3301 gcgcacatcg gcgacggcac gctcggggaa ggggtggtct acgagaccgc caacatcgtc
     3361 tcgttgcgcg gcctaccgct gctcgtcgtg ctggagaaca acggcgtcgc gcagtcgacc
     3421 gacacgaaga ccaccacggc tggcgacatc gagacccgct tcgccgcgtt cggcatcgac
     3481 gtcgatcgcc gctcggatgc cgacccggtg gggctggcgg accacttcgc cgaagtggtg
     3541 gagcgggtgc gagccggccg tcccttcgtt caggtggtcg acaccttccg cttgatggcc
     3601 cacagcaagg gcgacgacga ccgcgatcgc gccgcgatcg acgaggcttg gcgcaacgac
     3661 tacttcgccc gcttgctcga ctcgggcgat cccgaggcct ccgcggcgtg ga
//
LOCUS       DASPGN010000556         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1157697, whole genome shotgun sequence.
ACCESSION   DASPGN010000556 DASPGN010000000
VERSION     DASPGN010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157697"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1430
                     /locus_tag="VD788_16045"
     CDS             120..1430
                     /locus_tag="VD788_16045"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV77824.1"
                     /translation="MAEHTLATMDFGDPTMVDRSLSDCVQGLIGSEILKIAGDIRQLK
                     AAGRSICNLTVGDFDARYFPIPEPLRDGIHEAYRQGETNYPPSDGVLPLRKSITEFVA
                     RQWGSRYPIESVLIASGARPILYGAYRCVLNPGDKVIYPVPSWNNNHYAWLNQAESVV
                     VTTRAEDGFMPSLEQLRPQLRDARLLTLNSPLNPTGTVIGERVLREIAEAVVEENERR
                     SRVGQAHLFLLYDQVYAALVFGGARHHMPTALVPECAPWVITLDGISKSFAATGLRVG
                     WVLAAPELTARMKDLVGHMGAWAPRPEQIALARFLDSPEAVDEFRRSMNERVRSRLDA
                     LYAGFEALRDEGYPVECVSPQGAIYLSLRLRLGGQTIRGRRLDTNEQIRKLLLDEAGL
                     AVIPFQAFGLLEESGWFRLSVGAVSPTEIDEVFPRVRALLDDLA"
     gene            complement(1495..1569)
                     /locus_tag="VD788_16050"
     tRNA            complement(1495..1569)
                     /locus_tag="VD788_16050"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1535..1537),aa:Met,seq:cat)
     gene            1658..1831
                     /locus_tag="VD788_16055"
     CDS             1658..1831
                     /locus_tag="VD788_16055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77825.1"
                     /translation="MDEAKCHECGKTIDDTTLTKCPTCFKYFCAEHHVVMSGRPFCSK
                     NCADFFFFGDPDE"
     gene            1859..>2686
                     /gene="hemB"
                     /locus_tag="VD788_16060"
     CDS             1859..>2686
                     /gene="hemB"
                     /locus_tag="VD788_16060"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HXV77826.1"
                     /translation="MFPVTRPRRLRRTAAIRRLVRQTSLDPGDFVSPLFVVNGEGVRD
                     EIESMPGNYRYSVDTLVEEVGQAWRDGIRSVLLFGIPDAKDEVGSEAYDDSAAVQRAV
                     RALKQSHPELIVMTDVCLCEYTSHGHCGVVEDGEVKNDPTLELLARAAVSHAAAGADV
                     VAPSDMMDGRVGRIREELDGAGYGEVVILSYAVKYASAFYGPFREAAGSAPQFGDRRS
                     YQMDPANADEAMFEVAMDLEEGADIVMVKPALPYLDIIRRVKERYEVPLAAYNVSGEY
                     "
BASE COUNT          485 a          840 c          899 g          462 t
ORIGIN      
        1 cggaccatcg ctcgacctcc cgtgaggcgc cgagtctagc acccgcgttc caagcccaac
       61 aaacgaaacc aacaacttcg atctagccta ccggcacgtc ggaagataga atcggcgcga
      121 tggctgaaca cacgctcgcg acgatggatt tcggcgaccc gaccatggtc gaccgctcgt
      181 tgtccgactg cgtgcaggga ttgatcggct cggagatcct caagatcgca ggtgacatcc
      241 gccagttgaa agcggccggc cggtcgattt gcaacctgac ggtcggggat ttcgacgcgc
      301 gctattttcc gattccggag ccgctacgcg acgggattca cgaggcctac cgacagggcg
      361 agacgaacta tccgccgtcg gacggagtct tgccattgag gaagtcgatc accgagttcg
      421 ttgcgcgcca gtggggatcg cgctacccga tcgaatcggt actgatcgcg agcggtgcgc
      481 ggccgattct ctacggggcc tatcgctgcg tgttgaatcc cggcgacaag gtaatctatc
      541 ccgtcccgtc gtggaacaac aaccactacg cctggctcaa ccaggcggaa agcgtcgtgg
      601 tgacgacccg tgccgaagac ggcttcatgc catcgctcga gcaactgcgg ccgcagttgc
      661 gggacgcgcg cctgctcacg ctgaacagtc cgctgaaccc gaccggcacg gtgatcggcg
      721 agcgcgtgct gcgcgagatc gccgaggcgg tggtcgagga aaacgaacgc cgttcgcgag
      781 tcgggcaggc gcatctgttc ctgctttacg atcaggtgta tgccgcactg gtgttcggcg
      841 gcgcccgcca ccacatgccg acggcgctcg tacccgagtg cgccccatgg gtcatcacgc
      901 tcgacggaat ctcgaaatcg ttcgcggcga cagggctgcg cgtcggttgg gtgttggccg
      961 ccccggagct gaccgcgcgg atgaaggacc tcgtcggaca catgggggcc tgggcgccgc
     1021 ggccggagca gatcgcgctc gcccgctttc tcgattctcc ggaggcggtc gacgagttcc
     1081 ggcggtcgat gaacgagcgg gtccgttcgc ggctcgacgc gctctacgcc ggcttcgagg
     1141 cgctgcgcga cgaaggatac ccggtggagt gcgtcagccc gcaaggtgca atctatctgt
     1201 cgttgcgcct gcgactcggc ggtcagacga tccgcggccg tcggctcgat accaacgagc
     1261 agatccgcaa gctgctgctc gacgaggccg gactggcggt gatcccgttc caggcgttcg
     1321 gcctgttgga agagagcgga tggttccgcc tgtcggtcgg tgcggtgtcg cccacggaga
     1381 tcgacgaggt ctttccgcgc gtgcgcgccc tgctcgacga cctggcctga ccggacgacg
     1441 accgccgccg tggtggtaag atgggaccga acggttcgac ggggccggca gcggtggtgg
     1501 cggcgcgtgg agttgaacca cggacctagg ggttatgaat ccctcgctct accgctgagc
     1561 tacgccgccg acttaccggg ttgaaggaac ggtactctag cggttcggct cgatcaaagc
     1621 aacagccacg cgaccgtccg acgcagaagg tcagtagatg gacgaagcca agtgtcacga
     1681 gtgcgggaag acgatcgacg acacgactct gaccaagtgc ccgacttgct tcaagtactt
     1741 ctgtgccgag caccacgtgg taatgagcgg ccggccgttc tgcagcaaga actgcgcgga
     1801 cttcttcttc ttcggagatc cggacgaata gaccgaaccc gaaccggagc gagacatcat
     1861 gttcccggtg acacgacccc gtaggctcag acgaaccgcg gctattagac ggttggttcg
     1921 ccagacctcg ctcgacccgg gcgacttcgt ctccccgcta ttcgttgtca acggcgaggg
     1981 cgtgcgcgac gagatcgagt cgatgcccgg caactaccgc tactcggtcg atacgctggt
     2041 cgaggaagtc ggccaggcct ggcgcgacgg gattcgctcg gtcctgctgt tcggtatccc
     2101 cgacgccaag gacgaggtcg ggtccgaggc ttacgacgat tcggcggcgg tgcaacgcgc
     2161 ggtgcgcgcg ctgaagcaga gtcaccccga gctgatcgtg atgaccgacg tctgtctttg
     2221 cgagtacacg agccacggcc actgcggcgt cgtcgaagac ggggaggtga agaacgatcc
     2281 gacgctcgaa ctgctcgcgc gagccgcggt ctcgcatgct gcggccggcg ccgacgtcgt
     2341 tgcgccatcc gacatgatgg acggccgcgt cggccgcatc cgcgaagagc tggacggcgc
     2401 cggctacggg gaggtcgtca ttctgtccta tgccgtaaag tacgcatccg ccttctacgg
     2461 gccgttccgc gaagccgcgg gctcggctcc gcagttcggt gatcgcaggt cgtaccagat
     2521 ggatccggcg aacgccgacg aagcgatgtt cgaggtggcg atggatctcg aggagggcgc
     2581 ggacatcgtg atggtcaagc ctgcgctacc gtatctcgac atcatccggc gggtgaagga
     2641 gcgctacgag gtgccgctgg ccgcctacaa cgtcagcggc gaatac
//
LOCUS       DASPGN010000557         8072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1627840, whole genome shotgun sequence.
ACCESSION   DASPGN010000557 DASPGN010000000
VERSION     DASPGN010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8072
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627840"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1170
                     /locus_tag="VD788_16065"
     CDS             190..1170
                     /locus_tag="VD788_16065"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXV77827.1"
                     /translation="MLWKGFQRPKRLEVEQSTLSATFGRFHAQPFERGYGTTIGNALR
                     RILLSSIEGAAVTAVKIEGVLHEFSSIPGVVEDTTDIILNLKQIPFRIHSEHPETLTL
                     SATGPGTVKAGDIKLTANVEVLDPEMPVCTLSEEGKIAMELRLQRGRSYVSADRNFDE
                     DLGIGYIPLDSVHSPVRKVNYRVEDARLGQTTDYDRLILEVWTDGTVTPQDAVAMAAK
                     LLKDHMAIFINFEEAPEEDLDFPSTDDERLMEQFNRSVDELELSVRSYNCLKNANIKT
                     IGDLITRTEAEMLKTKNFGRKSLNEIKDILAEMGLSLGMELPGKVKTRMP"
     gene            1203..1586
                     /gene="rplQ"
                     /locus_tag="VD788_16070"
                     /pseudo
     CDS             1203..1586
                     /gene="rplQ"
                     /locus_tag="VD788_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017042856.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            1862..5110
                     /gene="carB"
                     /locus_tag="VD788_16075"
     CDS             1862..5110
                     /gene="carB"
                     /locus_tag="VD788_16075"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXV77828.1"
                     /translation="MPKRNDIRKILVIGSGPIVIGQACEFDYSGTQACKALREEGYEV
                     VLVNSNPATIMTDPAVADRTYVEPLDPSTVESILERERPDALLPTVGGQTALNLAMTL
                     GRKGVFDRLGIELIGADLDAIEMAEDREAFKEAMTEIGLATPLSGTARDLDEARRLVR
                     EIGFPVIIRPSFTLGGTGGGTAYNVEEFEQIVATGLDASPTTEVLVEQSVLGWKEFEL
                     ELMRDLNDNVVVICSIENFDAMGVHTGDSITVAPAQTLTDKEYQRLRNAAIRVIRKIG
                     VETGGSNIQFAVNPVDGEVVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITRETPSSFEPALDYVVVKIPRWAFEKFPASEPVLGTQMRSVGEAMAIGRTFKE
                     ALQKAIRSLDIGRYGLAADERHPIDPSRLRERLVTPTPDRLFYIHHAFQCGMNVEAIG
                     ELTAIDPWFLRQIEQLVDLEGRLRSFSLDTLPRPLLLRAKRDGFADRELARLLGCDEQ
                     RVRRRREADGVAPGFKRVDTCAAEFEAMTPYLYSSYEDECEATPTERRKVMILGGGPN
                     RIGQGIEFDYCCCHASFALRDEGVESIMVNCNPETVSTDYDTSDRLYFEPLTLEDVLR
                     IVEVEKPEGVIVQFGGQTPLGLALPLQQAGVTILGTSPDSIDLAEDRRRFGDLLARLE
                     IPAPNWDTARSFDEARLAAARIGYPVLVRPSYVLGGRAMLITHDESTLAETMKSAIAA
                     SPEHPVLLDQFLEDAFEVDVDAIADGEDVVVAGIMQHIEEAGVHSGDSACVIPPFHPV
                     VVERLDLMRDYTRRMAQALDVRGLMNVQFAIKDGVVYVLEVNPRASRTVPFVAKATGV
                     PVAGIAAKVMLGRRLRELGLVDEPCVSGHFVKEAVFPFARFPGEDPVLGPEMKSTGEV
                     MGTAAEFGMAFAKAQIAAGTPFPLSGTAFLSVNQNDKDGLLPIARGLAEQGFSLVATT
                     GTGDFLRANGLECQNVYKVLEGRPNVVDRIKNGEIHLILNTPLGRESYFDEKAMRRAA
                     TIRAIPLITTLSAGRAAVEAIRALKSGGYGVRSLQEIYANAPGRRGGLGSAG"
     gene            complement(5114..5596)
                     /locus_tag="VD788_16080"
     CDS             complement(5114..5596)
                     /locus_tag="VD788_16080"
                     /inference="COORDINATES: protein motif:HMM:NF013621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXV77829.1"
                     /translation="MSSADKVVDLATLSRRVAAARVAGRSVALANGLFDVLHVGHLRY
                     LEGAAREASLLVVALNSDASARRLKGPSRPIVPQHERAELVAGLACVDYVTVFDEDGA
                     EAMLERLRPDVHCKGTDYTVDNVPEAPVARALGIRIAIVGDAKAHATRDLIRRIRGGS
                     "
     gene            complement(5593..6612)
                     /locus_tag="VD788_16085"
     CDS             complement(5593..6612)
                     /locus_tag="VD788_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXV77830.1"
                     /translation="MSAAPAARARLVALVDAFAGRRVAVLGDLLVDEFLHGDIARVSR
                     EAPVLILEHRRTVAVPGGGANSVVNLRALGARPLPVGVIGRDAAGRRLVRVFRELGID
                     TRSLVEVDGYETPSKCRVLAGGTHTRRQQIVRVDYGAGRGEFPRRTQAMLRRALRHTL
                     LRADGLLIADYGFGSAAPEVAEVVIPPVRRRGLPVTVDSRSRVARFSGVSACTPNQEE
                     LELAFGRAPLDGGDLSRAARQLLRRTGNDAVLVTRGAEGMALFPRGGRRVDIPAFGTD
                     EVADVTGAGDTVIATFTLALLAGGNAEEAARIANYAAGIVVTKAGTATTSPRELIAAI
                     GEDLG"
     gene            complement(6609..7211)
                     /gene="gmhB"
                     /locus_tag="VD788_16090"
     CDS             complement(6609..7211)
                     /gene="gmhB"
                     /locus_tag="VD788_16090"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HXV77831.1"
                     /translation="MVVRADRRRAVLVDRDGTVCEEVGYVDSPEQLRLIPRSAEAIAA
                     AAAAGYQVVLVTNQGGIARGLLDEERLAEIHDHLRELLGEHGARLDGIYYCPHHPDGD
                     HPRYGVRCGCRKPAPGLLFRARDEMGIDLERSFMIGDDARDVEAGASAGVTPILVATG
                     HGQVQRDDPATRERLASVHLAADLEHAVDWILRREPPEPV"
     gene            complement(7262..>8072)
                     /locus_tag="VD788_16095"
     CDS             complement(7262..>8072)
                     /locus_tag="VD788_16095"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV77832.1"
                     /translation="LACGSLAGRLGYLLDGRHRRITLDNLRAAYGDAVDERWRRRVAR
                     RCWRHFGRITFDSLSIYALPRRRLDALLSYEGLEHVREAYARGRGVLFVSGHFGHWEL
                     TALMQGYLGFPLALVARRLDNPELERILIDLRQRSGNRVIHKHDALREMLRALREKLG
                     VALLIDQDARGDGIFVPFFGRPASTTPALGLLAIRTGAPVIPTFTVPLDDGTYRVHYG
                     RPVEPRHTGDRQQDAAWLTARCTEIIEEWVRRHPEAWLWMHRRWKTRPPPN"
BASE COUNT         1383 a         2689 c         2819 g         1181 t
ORIGIN      
        1 acggccaagg ggcgcggcgt gccgtcctgg ctcgagctcg acgcggaccg gtttcaggga
       61 acggtccgcc agctgcccga gcgcgaggag atcacgatgc cgatccagga gcagctgatc
      121 gtcgagctgt actccaagta acacgagggg acgcctcgtc atcggggcca cgccccaggg
      181 aggaattgga tgctctggaa aggattccaa cggccgaagc ggctcgaggt cgagcaaagc
      241 acgctctcgg ccaccttcgg caggttccac gcccaaccgt tcgagcgcgg ctacggaacc
      301 acgatcggga acgcgctgcg gcggatcctg ctctcgtcga tcgagggtgc cgcggtcacc
      361 gcggtcaaga tcgaaggcgt gctgcacgag ttctcgtcga taccgggcgt ggtcgaggac
      421 acgaccgaca tcatcctcaa tctcaagcag atcccgttcc gtatccacag cgagcacccg
      481 gaaacgctga cgctgagcgc gaccgggccc ggcacggtca aggccggtga catcaagctg
      541 accgcgaacg tcgaggtgct cgaccccgag atgccggtct gcaccttgtc cgaagagggc
      601 aagatcgcga tggagctgcg tctccagcgg ggtcggagct acgtcagcgc cgaccgcaac
      661 ttcgacgagg atctcggcat cggctacatc ccgctcgatt cggtccattc gccggtgcgc
      721 aaggtgaact atcgggtgga ggacgcccgt ctcgggcaga cgaccgacta cgaccggctg
      781 atcctcgagg tctggaccga cggtacggtg acgccgcagg acgcggtggc gatggccgcc
      841 aagctgctca aggaccacat ggcgatcttc atcaacttcg aggaggcgcc ggaagaggac
      901 ctcgacttcc cgtcgaccga cgacgagcgg ctgatggagc agttcaatcg ttcggtcgac
      961 gagctggagc tctcggttcg atcgtacaac tgcctgaaga acgccaacat caagacgatc
     1021 ggcgatctga tcacgcgtac cgaagcggag atgctgaaga cgaagaactt cggtcgcaag
     1081 tcgctgaacg aaatcaagga catcctcgcc gagatgggac tttcgctcgg tatggaactt
     1141 cccgggaagg tcaagacgcg gatgccctga tccgccgccc acccgatgac ggagagtcgc
     1201 cgatgaaaca ccgcgtagcc ggacggaagc tcggccgaac cagcgagcat cgactggcac
     1261 tgatgaggaa tctgtcgacg gcgctgtttg aaagagagaa gatccgcacg acgctggcca
     1321 aggccaagga gctgcgtccg ttcgccgagc gactcgtcac gctgtccaag cgcgagaccc
     1381 tgcacgcgcg tcgcctggtc ctgcgccaca tcaaagaccg gaaggtcgtc ggcaagctgt
     1441 tcgattcgct gtcggcacgg ttcgcccagc ggccgggcgg ctacacgcgc atcatcaaac
     1501 tcgggccgcg gcggggagac aacgccgaga tggcgatcgt cgaactggtc ggcgcggaac
     1561 cggaagtgcg ggaggccaaa gcggagccga agaagaaaaa gggcggaaag cccaagacga
     1621 ccacggatgc ccccgccgcc gaggtggcct cgaccccgaa gaagaagacg aagacgaaga
     1681 ccaaggccag aaagacgccg gtcaggaaga gcaaggcagc ggtgtccaag aagaaatcga
     1741 gcacgaagag aaaagccggc tcgggagagt aggttggtcg gtcgacgacg catcgatcgc
     1801 ccaccgccga cgagggagcc agggtgctcc ctctttttgc atctgacggg acggaaacgg
     1861 cgtgcccaaa cgcaatgaca ttcgcaagat cctcgtgatc ggctccggtc cgatcgtcat
     1921 cggtcaggcc tgcgagttcg actactccgg cacgcaagcc tgcaaggcgc tgcgggaaga
     1981 gggatatgag gtcgtgctgg tgaactccaa cccggcgacg atcatgaccg accccgcggt
     2041 cgccgaccga acctacgtcg agccgctcga cccgagcacc gtcgagagca tcctcgagcg
     2101 cgagcgtccc gacgcgctcc tgccgaccgt cggcgggcag accgcattga acctggcgat
     2161 gacgctcggg cgcaaaggcg ttttcgatcg tctcggcatc gagctgatcg gcgccgatct
     2221 cgacgcgatc gagatggccg aggaccgcga ggcgttcaag gaggcgatga ccgagatcgg
     2281 tctggccacg ccgttgagcg gcacggccag ggatctcgac gaggcgcgcc ggctggtgcg
     2341 cgagatcggt tttcccgtca tcatccggcc gtccttcact ctcggcggga cgggcggcgg
     2401 tacggcctac aacgtcgaag agttcgagca gatcgtcgcc accgggttgg acgccagtcc
     2461 gacgaccgag gtgctggtcg agcagtcggt gctgggctgg aaggagttcg agctcgagct
     2521 gatgcgagat ctcaacgaca acgtggtcgt catctgctcg atcgagaact tcgacgcgat
     2581 gggtgtgcac accggcgatt cgatcaccgt cgcgccggcg cagaccctga ccgacaagga
     2641 ataccagcgc ctgcgcaacg cggcgattcg cgtgatccgc aagatcggtg tcgagaccgg
     2701 cggatcgaac atccagttcg cggtcaaccc ggtcgacggc gaggtcgtcg tcatcgagat
     2761 gaacccccgc gtgtcgcgct cgtccgctct cgcgtccaag gcgaccggct tcccgatcgc
     2821 gaagatcgcg gccaagctgg cggtgggcta cacgctggac gagatcccga acgacatcac
     2881 gcgcgagacg ccgtcctcgt tcgagccggc gttggactat gtggtggtca agatcccgcg
     2941 ctgggcattc gagaagttcc cggcgtccga gccggtgctc ggcacccaga tgagatcggt
     3001 cggcgaggcg atggcgatcg gccggacctt caaggaggca ctccagaagg cgatccgctc
     3061 gctcgacatc ggccgctacg gtctggcggc cgacgagcgg cacccgatcg atccctcgcg
     3121 gctgcgtgag cggctggtca cgccgacccc ggatcgcctg ttctacatcc accatgcgtt
     3181 ccagtgcggt atgaacgtcg aggcgatcgg cgagctgacc gcgatcgatc cgtggttcct
     3241 gcgccagatc gagcagcttg tcgatctcga gggccggctg cgctcgttct cgctcgacac
     3301 gctgccgcga ccgctgttgc tgcgtgccaa gcgggacggg ttcgccgacc gggagctggc
     3361 ccggttgctc ggctgcgacg agcaacgggt gcgccggcgg cgcgaggcgg acggcgtggc
     3421 gcccggcttc aaacgggtcg acacctgcgc cgccgagttc gaggcgatga caccttacct
     3481 ctattcgagc tacgaggacg agtgcgaggc gacgccgacc gaacgccgca aggtgatgat
     3541 tctcggcggt gggccgaacc gcatcggtca agggatcgag ttcgactact gttgctgcca
     3601 cgcctcgttc gcgctgcgcg acgagggggt cgaaagcatc atggtcaact gcaatcccga
     3661 aacggtgtcg accgactacg acacctccga ccgcttgtat ttcgagccgc tgacgctgga
     3721 ggacgtgttg cggatcgtcg aggtcgagaa gcccgagggc gtcatcgtcc agttcggcgg
     3781 gcagacgccg ttggggctgg cgctgccgct gcagcaggcc ggagtgacga tcctcggcac
     3841 ctcgcccgac tcgatcgact tggccgagga ccggcggcgc ttcggcgacc tgctggcacg
     3901 gctcgagatt cccgcgccga actgggatac ggcgcgttcg ttcgacgagg cacgcctcgc
     3961 ggcggcccgc atcggctacc cggtgctggt ccggccgtcc tacgtgctgg gcgggcgcgc
     4021 gatgctgatc acccacgacg aatcgacgct ggcggaaacg atgaagagcg cgatcgccgc
     4081 ctcgcccgag catccggtac tgctcgacca gttcctcgag gacgcgttcg aagtcgacgt
     4141 ggatgcgatc gcggatggcg aggatgtcgt cgttgccggc atcatgcagc acatcgagga
     4201 ggcgggcgtg cactcgggtg actcggcctg cgtgataccg ccgttccacc cggtcgtggt
     4261 cgaacgtttg gacctcatgc gcgactacac gcgccggatg gcgcaggcgc tcgacgtccg
     4321 cgggctgatg aacgtgcaat tcgcgatcaa ggacggtgtg gtctacgtgc tcgaggtgaa
     4381 ccctcgcgcc agtcgcacgg tgccgttcgt ggccaaggcg accggggtgc cggtcgccgg
     4441 gatcgcggcg aaggtcatgc tcggccgacg actgcgcgag ctggggttgg tcgacgagcc
     4501 gtgcgtcagc gggcatttcg tcaaggaggc ggtcttcccc ttcgcccggt tccccggcga
     4561 agatccggtg ctcggtcccg agatgaagag caccggcgag gtgatgggca cggccgccga
     4621 gttcgggatg gcgttcgcca aggcgcagat cgcggcgggg acgccgtttc cactgtcggg
     4681 gacggcgttc ctctcggtca accagaacga caaggacggt ctgctgccga tcgcgcgcgg
     4741 gctggccgag cagggcttca gcctcgtcgc gacgaccggc accggcgatt tcctgcgggc
     4801 caacgggctc gagtgccaaa acgtctacaa ggtgctcgag ggtcggccga acgtcgtcga
     4861 ccggatcaag aacggggaga tccatctgat cctgaacacg ccgctcggac gcgagtcgta
     4921 cttcgacgag aaggcgatgc ggcgtgccgc gacgatccgt gcgatcccgc tgatcaccac
     4981 cctgtccgcg gggcgggcgg cggtcgaggc gattcgcgcg ctgaagagcg ggggctacgg
     5041 ggtgcgcagt ttgcaggaga tctacgcgaa cgcgccgggg cggcgcgggg ggctcggctc
     5101 cgccggctga gcctcacgac cccccgcgga tcctccggat cagatcgcgc gtcgcgtgcg
     5161 ccttggcgtc gccgacgatc gcgatgcgga tgccgagtgc gcgggcgacg ggagcctcgg
     5221 gcacgttgtc gacggtgtag tcggtaccct tgcagtggac gtccggccgc aatcgctcga
     5281 gcatcgcctc ggcgccgtcc tcgtcgaaga ccgtgacgta gtcgacgcag gcgagcccgg
     5341 caacgagctc ggcgcgctcg tgctgcggga cgatgggtcg ggacgggccc ttgagtcttc
     5401 gcgccgaggc gtccgagttg agcgcgacga cgagcaggct tgcctcgcgc gccgcaccct
     5461 cgagatagcg caagtggccg acgtgcagca cgtcgaacag gccgttggcc aacgcgaccg
     5521 accgccccgc gacacgcgcc gccgccacgc ggcgcgacag cgttgcgaga tcgaccactt
     5581 tgtcggcgct gctcatccga gatcctcccc gatcgccgcg atcagttcgc gcggtgacgt
     5641 cgtcgccgta ccggccttgg tcacgacgat gccggcggcg tagttggcga tgcgggccgc
     5701 ctcctcggcg ttcccgccgg ccaacagcgc gagggtgaag gtcgcgatga ccgtatcgcc
     5761 cgcgccggtg acatcggcga cctcgtcggt gccgaaggcc gggatgtcga cgcgccggcc
     5821 gccgcgtggg aacagcgcca tgccctcggc accccgcgtg accagcaccg cgtcgtttcc
     5881 cgtacgacgc agcagctgtc gtgccgcccg cgacagatct ccgccgtcca acggcgctcg
     5941 gccgaacgcc agctcgagct cttcctggtt cggcgtgcag gcgctgacgc ccgagaaccg
     6001 ggcgacgcgg ctgcgcgagt cgacggtgac cggcaggcct cggcgacgca ccggcggaat
     6061 cacgacttcc gcgacctcgg gggcggccga gccgaatccg tagtcggcga tcagcaggcc
     6121 gtcggccctc agcagagtat ggcgcagcgc gcggcgcagc atggcctgcg tacggcgggg
     6181 gaactcgccg cgccctgcgc cgtagtcgac gcgcacgatc tgctggcggc gcgtgtgcgt
     6241 cccgccggcg agcacccggc acttggacgg agtctcgtag ccgtcgacct cgacgagcga
     6301 gcgcgtgtcg atacccagct cgcgaaagac gcgaaccagc cggcgcccgg ccgcatcccg
     6361 cccgatgacg ccgaccggca gcgggcgggc gccgagcgcc cgcagattga cgacgctgtt
     6421 cgcgccgccc cccgggacgg cgacggtgcg ccggtgctcg aggatcagca ccggggcctc
     6481 gcgcgagacg cgcgcgatgt ccccgtggag aaactcgtcg acgagcaggt cgcccaagac
     6541 ggcgacgcgc cgtccggcga aggcgtcgac cagcgcgacc aagcgcgctc gtgcggcggg
     6601 agccgcgctc acacgggctc cggcggctcg cggcgcagga tccagtcgac ggcgtgctcg
     6661 aggtccgcgg cgaggtgaac cgaggcgagc cgctcccggg tcgccggatc gtcgcgttgg
     6721 acttgtccat gtcccgtcgc caccaggatc ggcgtgacgc cggcgctcgc gccggcctcg
     6781 acgtcgcgcg cgtcgtcgcc gatcatgaac gagcgctcga ggtcgatccc catctcgtcg
     6841 cgcgcccgga acagcagccc gggggccggc ttgcgacagc cgcaacgcac gccgtagcgc
     6901 ggatggtcgc cgtccgggtg gtgcgggcag tagtagatcc cgtcgagccg cgcgccgtgt
     6961 tcaccgagca actcgcgcag gtgatcgtgg atctcggcca accgctcttc gtcgagcaga
     7021 ccgcgcgcga tcccgccctg gttggtgacc agcacgacct ggtacccggc cgccgccgcc
     7081 gccgcgatcg cctcggcgct gcggggaatc aaccggagct gctcgggcga atcgacgtag
     7141 ccgacctcct cgcagacggt cccgtcgcga tcgaccagaa cggcgcgtct cctgtcggcc
     7201 cgtaccacca ttcggcgagt ctagcaggcc gcgccgaccg gcggtacggc gcggctcgcc
     7261 gtcaattcgg cgggggacgc gtcttccagc ggcgatgcat ccacagccac gcctcggggt
     7321 gccgtcggac ccactcctcg atgatctcgg tgcagcgcgc cgtcagccac gccgcgtcct
     7381 gctggcggtc tcccgtgtga cggggctcga cggggcgtcc gtagtggacg cggtaggttc
     7441 catcgtcgag cggcacggtg aacgtcggga tcaccggcgc gccggtgcgt atcgcgagca
     7501 agccgagcgc cggggtcgtc gaggccggac ggccgaagaa cgggacgaag atcccgtcgc
     7561 cccgtgcgtc ctggtcgatc aacagcgcga cacccagctt ctcgcgcaac gcgcgcaaca
     7621 tctcgcgcag cgcgtcgtgc ttgtggatca cccggttgcc cgagcgctgg cgcaggtcga
     7681 tcaggatgcg ctcgagctcg gggttgtcga gccggcgcgc gacgagggcc aacgggaagc
     7741 cgaggtagcc ctgcatcagc gccgtcagct cccagtggcc gaagtgcccg gagacgaaca
     7801 gcacacctcg cccccgcgcg tacgcctcgc gcacgtgctc cagaccctcg tagctcaaga
     7861 gcgcgtcgag ccgccgccgc ggcaatgcgt agatcgacaa tgaatcgaag gtgatccggc
     7921 cgaagtggcg ccagcagcgt cgcgcgaccc gtcggcgcca gcgctcgtcg accgcatccc
     7981 cgtaggccgc gcgcagattg tcgagcgtga tccggcggtg gcggccgtcg agcaggtagc
     8041 ccagtcggcc ggcgagcgag ccgcaggcca gc
//
LOCUS       DASPGN010000558         6347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_105221, whole genome shotgun sequence.
ACCESSION   DASPGN010000558 DASPGN010000000
VERSION     DASPGN010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105221"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VD788_16100"
     CDS             complement(<1..593)
                     /locus_tag="VD788_16100"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HXV77833.1"
                     /translation="MKVLAVDLTRQPRRSESIAHVLVLLDEHGRTAIQQRLPTLPAVA
                     AAVAELAGDEPFLLGVNTPVVVPAKAARGRPVENLVRRQFRYRLPPGGRASLQAEPLG
                     VAGEALIAGLAAAGLASLPYPDRDRRRSGLVETYPPLILKALLWDSSILRSIGDVATR
                     EELLRAFNPPPYRRADLPARATWADQAAALELVLRSLG"
     gene            complement(602..1942)
                     /gene="glmM"
                     /locus_tag="VD788_16105"
     CDS             complement(602..1942)
                     /gene="glmM"
                     /locus_tag="VD788_16105"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXV77834.1"
                     /translation="MKRLFGTDGIRAVAGDPPLDPPTVRKFGAALATVLGRGPEAGGR
                     CRVVLGRDTRESGPSLRDAVVAGLAARRAVAVDAGVITTPGLAHVVLHGGFDAGVMIS
                     ASHNPFEDNGLKAFGRDGFKLPDDTEQQIERLMLDGGLDDPGEAAARVEADPLLLDEY
                     VRSLERAAPNRLDGLDVVLDCANGAASAIAPELFRRRGARVTTLATEPDGRNINLDCG
                     SLHLERLGQTVRRSGADLGLAFDGDADRCLAVDRRGRTVDGDHILYLAGCRMKRNGTL
                     RRNALVATIMSNFWLEERLGFEGIGLHRAPVGDKYVLERMIREDLVLGGEQSGHVIFR
                     DHASTGDGILTGLLLLDAVTDGPPLEQVMDGIEPYPQVLINVRVKSKPDLDRHARIGP
                     AGRRTEAELDGHGRVVLRYSGTEPKARVMVEGKHAETVRRLAESLSALIEEELG"
     gene            complement(1943..2890)
                     /locus_tag="VD788_16110"
     CDS             complement(1943..2890)
                     /locus_tag="VD788_16110"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HXV77835.1"
                     /translation="MLDTLVANWPLKLLALVLAFAIWVSVTGENRVVQDFKVPLEVRL
                     PASYVLASHPPTTVDVRLRGAESVMRRLEPLPMLVRVDLDDPAVGTREVLLSETHLSG
                     VPKGVDVDFIDPSRTTITIEERSQREVTLEPTFLGQPAEGFTLYNARLSPSRVRVEGP
                     VSDVDRLEVLRTTPIRLDGRSEPFVARVSVVPEGDFVRLMDSMTVNVRVVVDMAAVER
                     PIKHIPIGIIGAGPGTTVAPSAVDVILSGPPRLLDLIDGDQVRPTVDAGQVATDSGRT
                     RLPIRIDLEGLTPDERSAVSIKSLSPRDAIVTVSGGRSL"
     gene            complement(2883..3725)
                     /gene="cdaA"
                     /locus_tag="VD788_16115"
     CDS             complement(2883..3725)
                     /gene="cdaA"
                     /locus_tag="VD788_16115"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HXV77836.1"
                     /translation="MIERLFTALHIEDLGPWAVVDIAILALLIYQLLLMIRGTRAVNI
                     LLALASVALVYVVTGPGLLQLNAVHGILGNLLFYLPLIVIVLFQNQIRHALAQLGKNP
                     LSGLIPRREDESLIEEVSLAAASLASKRVGALIIVEREMGLRTFADTGIALDALVSYD
                     LLMNLFTRRSPLHDGAVIIAEGRIKAASCYLPLTTNPSLSRTYGTRHRAAIGITEESD
                     ALAIVVSEERGVISLAEGGKISGGLDARRLQAALELALGGANEREPSGRGRQIVATTG
                     ESDA"
     gene            complement(3732..4583)
                     /gene="folP"
                     /locus_tag="VD788_16120"
     CDS             complement(3732..4583)
                     /gene="folP"
                     /locus_tag="VD788_16120"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXV77837.1"
                     /translation="MDVRPGYEIRFADGGSMPLGARTRVMGVLNVTPDSFSDGGRHAD
                     AGRAADAARRMIEEGADLIDVGGESSRPGAQPVDAAAEIERVVPVIERIRRASDVRLS
                     IDTTKPEVAVRALDAGADVVNDIGACRDPKMLELVLERRVPVVLMHMRGDPPTMQDDT
                     AYDDVVREVVAYLEDRVDRAIAAGVKDGKILVDPGIGFGKSTSGNLSILRQLSSLNRV
                     GRPIVVGASRKTFIGRTLNLPVEERLEGSLAAAALASAHGAHVIRAHDVEATVRVVRM
                     IDAIRGA"
     gene            complement(4632..>6347)
                     /gene="ftsH"
                     /locus_tag="VD788_16125"
     CDS             complement(4632..>6347)
                     /gene="ftsH"
                     /locus_tag="VD788_16125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXV77838.1"
                     /translation="QYEGLANKLIAQGILVTAKEPTQSPWAQLLYSWAPILLLIGFWI
                     FFMRQMQSGGNKALSFGKSKAKLSSSSQKKVTFKDVAGVDEAKEELQEIIEFLKEPQK
                     FQKLGGRIPKGVLLMGPPGTGKTLLARAVAGEANVPFFSISGSDFVEMFVGVGASRVR
                     DLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFETNEGVI
                     LIAATNRPDVLDPALMRPGRFDRQVVVGRPDVRGREEIIKVHVRKIPLAAEIDLSLIA
                     RATPGFSGADLANLVNEAALYAARKNRKAVTQEDFEVAKDKVLMGTERKSLIISEDEK
                     RATAFHEAGHALVAYSLPNADPLHKVTIIPRGRALGVTQQLPVDDRHTYSRDYLETTI
                     AVLMGGRVAEELKLNRVTTGAGNDFERSTEVARKMVCEWGMSERMGPLTFGKQEEQIF
                     LGREIAQHRDYSEATAVEIDHEVKRIVTEGYNRARKILEDQDEQLTRLAAALLEREVL
                     DAGQIAAIAEGRSLEPLGAGSPSGDRSTGFAAKAGAGSGTADKVERDTGKTPGVLPEP
                     GNQPA"
BASE COUNT          985 a         2224 c         2133 g         1005 t
ORIGIN      
        1 ccgagggagc gcagcacgag ctcgagcgcc gccgcctgat cggcccacgt cgcgcgcgcg
       61 ggaaggtcgg cgcggcggta gggcggcggg ttgaacgcgc gcaacagctc ctcccgcgtc
      121 gccacgtcgc cgatcgagcg caggatcgac gaatcccaca gcagggcctt gaggatcagc
      181 ggcgggtagg tttctaccag gccgctgcgg cgccggtcac ggtcggggta gggcagggac
      241 gccaagccgg cggcggccag cccggcgatc agcgcctcgc cggcgacacc gagcggttcg
      301 gcctgcagcg aggcgcgccc ccccggcggc agtcggtaac ggaactgacg gcggaccagg
      361 ttctcgaccg ggcggccgcg cgccgctttg gccgggacga cgaccggcgt gttgaccccc
      421 agcaggaacg gctcgtcgcc ggccagttcg gcgaccgccg ccgcgaccgc gggaagcgtg
      481 ggcaggcgtt gttggatcgc ggtgcgaccg tgctcgtcga gcagcacgag cacgtgggcg
      541 atcgactccg aacgccgtgg ctgacgcgtc aggtcgacag cgagaacttt cacgctggcc
      601 cctaccccag ctcttcctcg atcaacgcgg acaggctctc ggccagacga cggacggttt
      661 ccgcatgctt gccctcgacc atcacccggg ccttcggttc cgtaccggag tagcgcaaca
      721 caacccgccc gtgtccgtca agctcggcct cggtccggcg cccggccgga ccgatccggg
      781 cgtggcgatc gagatcgggt ttggatttga cgcgtacgtt gatcaagacc tgggggtagg
      841 gctcgatgcc gtccatcacc tgttcgagcg gcggcccgtc ggtgacggcg tcgagcagca
      901 gcaagccggt gaggatgccg tcgccggtcg aggcgtgatc gcgaaagatg acgtggcccg
      961 actgctcgcc gccgagcacg agatcttctc gtatcatccg ctcgaggacg tacttgtcgc
     1021 cgaccggcgc ccgatgcagg ccaatcccct cgaaccccag tcgctcctcg agccagaagt
     1081 tgctcatgat cgtcgcgacc aacgcgttgc gacgcagcgt tccgttccgc ttcatccggc
     1141 agccggcgag gtagaggatg tggtcgccgt cgaccgtccg gccgcgccgg tcgaccgcga
     1201 gacagcgatc ggcgtcgccg tcgaacgcca gaccgagatc ggcgcccgag cggcgtacgg
     1261 tctggccgag ccgctcgagg tgaagcgagc cgcagtcgag attgatgttg cgcccgtcgg
     1321 gttcggtcgc gagggtcgtc acgcgagccc cgcgccgtcg gaacagctcg ggcgcgatcg
     1381 cgctggccgc cccattcgcg caatcgagga cgacgtcgag gccgtccaac cggttggggg
     1441 ccgctcgttc gagcgaccgg acgtactcgt cgagcagcag cgggtcggcc tcgacgcggg
     1501 ccgctgcctc gcccgggtcg tcgagcccgc cgtccagcat caaccgctcg atctgctgtt
     1561 cggtgtcgtc cggcagcttg aatccgtcgc ggccgaacgc cttgagcccg ttgtcctcga
     1621 acggattgtg gctggccgag atcatcaccc cggcatcgaa tccgccgtgc agcacgacgt
     1681 gcgccaggcc gggggtggtg atgacgcccg catcgacggc gaccgcccgt cgcgccgcga
     1741 ggccggccac gacggcgtcg cgtagcgacg ggccggactc ccgcgtgtcg cgtcccaaca
     1801 cgacgcgaca gcggccgccg gcctcgggcc cgcgccccag cacagtggcc agcgccgcac
     1861 cgaacttgcg aaccgtcgga ggatcgagcg gagggtcacc ggcgacggcg cgaatgccgt
     1921 ccgtgccgaa caggcgtttc attcacagac tcctgccacc agacaccgtg acgatcgcgt
     1981 cccgtgggct gagcgacttg atgctcacgg cggagcgttc gtccggcgtc aggccttcca
     2041 ggtcgatgcg aatcggcagc ctcgtccgtc ccgagtcggt cgcgacctgg ccggcgtcga
     2101 cggtcggccg aacctggtcg ccgtcgatca ggtcgagcag ccgcggtgga ccggacagta
     2161 tcacgtcgac ggccgacggg gcgacggtcg tccccggacc ggcgccgatg atgccgatcg
     2221 ggatgtgctt gatcgggcgc tcgaccgccg ccatgtcgac cacgacccgc acgttgaccg
     2281 tcatcgagtc catcaaccgc acgaaatcgc cttcgggcac gacggacacc cgggcgacga
     2341 acggttcgct acgcccgtcc agacggatcg gcgtcgtgcg caacacctcc agtcgatcga
     2401 cgtcggagac cgggccctcg acgcggacgc ggctgggcga cagccgcgcg ttgtacagcg
     2461 tgaaaccttc agccggttgc ccgaggaagg tcggctcgag cgtcacctcc cgttgcgagc
     2521 gctcctcgat cgtgatcgtg gtccgcgacg gatcgatgaa gtcgacatcc acgcccttcg
     2581 ggacgccgct caagtgggtt tcggacagca gcacctcgcg ggtgccgacg gccgggtcgt
     2641 cgagatcgac ccgcaccagc atcggcagcg gctcgaggcg gcgcatgacg ctttccgccc
     2701 cgcgcagtcg gacgtcgacg gtggtcggcg ggtgcgaggc gagcacgtag ctcgccggca
     2761 agcggacctc gagcggcacc ttgaaatcct ggacgacgcg gttctcgccg gtgaccgaga
     2821 cccagatcgc gaaggccagc accagcgcga gcagcttgag cggccagttg gcgacgagcg
     2881 tgtcaagcat cggactcacc cgtcgtcgcg acgatctgcc ggccccgccc ggacggctcc
     2941 ctctcgttgg cgccgcccag cgccagctcg agcgcggcct ggagtcgcct ggcgtccaaa
     3001 ccacccgaga tcttgccccc ctcggccaac gagatcacgc cgcgctcctc ggacacgacg
     3061 atcgccagcg cgtccgactc ttccgtgatg ccgatcgccg cccggtgacg cgtgccgtag
     3121 gtgcgcgaca ggctggggtt cgtcgtcagc ggcaagtagc acgaagccgc cttgatccgt
     3181 ccctcggcga tgatcacggc gccgtcgtgc agcggcgagc gacgggtgaa caggttcatc
     3241 agcaggtcgt aggagaccaa cgcgtcgagc gcgatgccgg tatcggcgaa cgtgcgtagc
     3301 cccatctcgc gctcgacgat gatcagcgca ccgacccgtt tggaggcgag cgacgccgcg
     3361 gccagcgaga cctcctcgat caagctctcg tcctcgcggc gcgggatcag accgctgagc
     3421 gggttcttgc cgagctgggc cagcgcgtgc cggatctgat tctggaacaa cacgatcacg
     3481 atcaacggaa ggtagaacaa caggttgccg aggatgccgt gaaccgcgtt gagctggagc
     3541 agaccggggc cggtgacgac gtagaccaac gcgacggaag ccagcgcgag caggatgttg
     3601 accgctcgcg tcccgcggat catcagcaac aactggtaga tcaacaacgc caggatggcg
     3661 atgtcgacga ccgcccacgg accgaggtcc tcgatgtgca gggcggtgaa cagtcgctcg
     3721 atcatgcggt gtcaggcccc ccggatagcg tcgatcatcc gcacgacccg cacggtggcc
     3781 tcgacgtcgt gcgcccggat gacgtgggcg ccgtgggcgc tggcgagtgc ggcggcggcc
     3841 aaactcccct ccaatcgctc ctcgaccgga aggttcaggg ttctcccgat gaacgtcttg
     3901 cgcgacgcgc cgaccacgat cggccgtccg acccggttca gcgacgagag ctgtctcagg
     3961 atcgacaggt tgccggaggt cgacttaccg aagccgattc caggatccac cagaatctta
     4021 ccatctttga cgccggcggc gatcgctcga tcgacccgat cctcgaggta ggcgacgacc
     4081 tcgcgcacga cgtcgtcgta ggcggtgtcg tcctgcatgg tcggcgggtc gccccgcatg
     4141 tgcatcagca ccaccggtac gcggcgctcc agcaccagct cgagcatctt cggatcgcga
     4201 caggcgccga tgtcgttgac gacgtcggcg ccggcgtcca gcgcccgcac cgccacctcg
     4261 ggcttggtcg tgtcgatcga caggcggacg tcggacgccc gacggattcg ctcgatcacc
     4321 gggacgacgc gctcgatctc ggcggccgcg tcgacgggct gcgcccccgg acgggagctc
     4381 tcaccgccga cgtcgatcag atcggcgccc tcctcgatca tcctccgggc ggcgtcggcc
     4441 gcgcgccccg cgtcggcgtg gcggccaccg tccgaaaacg agtcgggagt gacgttcagc
     4501 acgcccatca cgcgcgtgcg cgcgccgagc ggcatcgagc cgccgtcggc gaaccggatc
     4561 tcgtagccgg gtcgaacgtc catattcctc cctgctggtc gtgccgacag ggagccggcg
     4621 gtcgttcgag ctcaggcagg ctggtttccg ggctccggca ggacgcccgg ggtcttgccc
     4681 gtgtcgcgct cgaccttgtc cgcggtcccg cttccggccc cggccttcgc cgcgaacccc
     4741 gtcgatcgat cgccggacgg ggagccggcg cccagcggct cgaggctccg gccttcggcg
     4801 atcgccgcga tctggcccgc gtcgagcacc tcgcgctcga gcagcgccgc ggccagccgg
     4861 gtcaactgct cgtcctgatc ctcgaggatc ttgcgcgcac ggttgtagcc ttccgtcacg
     4921 atgcgcttga cctcgtggtc gatctcgacg gcggttgcct cgctgtagtc gcggtgctgg
     4981 gcgatctcgc ggccgaggaa gatctgctct tcctgcttgc cgaaggtgag cggacccatt
     5041 ctctcggaca tcccccactc gcacaccatc ttgcgcgcca cctcggtcga acgctcgaag
     5101 tcgttgcccg cgccggtggt cacccggttg agcttcaact cctcggcgac ccgaccgccc
     5161 atcagcacgg cgatcgtcgt ttcgaggtag tcgcgggagt aggtgtggcg gtcatcgacc
     5221 ggtagctgct gggtcacgcc cagcgcgcgg ccgcgcggga tgatcgtcac cttgtgcagc
     5281 ggatcggcgt tgggaagcga gtaggccacc agggcgtgac cggcctcgtg aaaggcggtc
     5341 gcccgtttct cgtcctcgct gatgatcagc gacttgcgct cggtgcccat cagcaccttg
     5401 tccttggcca cctcgaagtc ttcctgcgtc accgccttgc ggttcttgcg cgcggcgtat
     5461 aacgcagcct cgttgaccag gttggctagg tccgcaccgg agaatccggg cgtggcgcgt
     5521 gcgatcaagc tcaggtcgat ctccgcggcc agcgggatct tgcgcacgtg gaccttgatg
     5581 atctcttcgc ggccgcgtac gtcgggccgg ccgacgacga cctgccggtc gaagcgaccg
     5641 ggccgcatca acgccgggtc gagcacatcg ggccgattgg tcgccgcgat caggatcacg
     5701 ccctcgttcg tctcgaagcc gtccatctcg accagcagct ggttcaacgt ctgctcgcgt
     5761 tcgtcgtgac cgccgccgag cccggctccg cggtgccgcc cgaccgcgtc gatctcgtcg
     5821 atgaagatga tgcacggcgc gttcttcttg ccctgctcga acaggtcgcg gacgcgggag
     5881 gcgccgacgc ccacgaacat ctcgacgaag tccgacccgc tgatcgagaa aaacggcacg
     5941 ttggcttcgc cggcgaccgc acgagcgagc agcgtcttgc cggtaccggg aggccccatg
     6001 aggagcacgc ccttcggaat gcggcccccg agcttctgga acttctgcgg ctccttcagg
     6061 aactcgatga tttcctgcag ttcttccttc gcttcgtcta cgccagcgac atccttgaag
     6121 gtgacctttt tctgcgagct cgacgacagc ttggccttgc tcttgccgaa cgacagcgct
     6181 ttgttgccgc cgctctgcat ctggcgcatg aagaagatcc agaagccgat gagcagcaga
     6241 atcggcgccc acgaatacag gagctgcgcc cacgggctct gcgtcggctc cttcgccgtc
     6301 accaggatgc cctgcgcgat caacttgttg gccaggccct cgtactg
//
LOCUS       DASPGN010000559         4443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_690248, whole genome shotgun sequence.
ACCESSION   DASPGN010000559 DASPGN010000000
VERSION     DASPGN010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690248"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VD788_16130"
     CDS             <1..498
                     /locus_tag="VD788_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77839.1"
                     /translation="LAAGDMDRAKANADFARAMGNADFARAMGNADFARAMGNADFAR
                     AMGNADFARALGNADFARALGNADFARALGNADFARALGNADFARALGNADFARALGN
                     ADFARALGNADFARALGNPDFARAMSHSDFARALGNPNFARALGNADFNRALASGDVA
                     QFAAD"
     gene            611..3559
                     /locus_tag="VD788_16135"
     CDS             611..3559
                     /locus_tag="VD788_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77840.1"
                     /translation="MRAPRAIVFCWVLLLSPFVHAQLKVVETEHLRLVYFDPVQAPLA
                     PYVAQSFENSMEFQRDLFDYEPSEKVTVFLSDIADYGNAGATAVPRNFVAVQVAPNSF
                     AYETYPSNERINTLMNHELVHVAAFDRPAGSDRTFRKLFRGKVYETADHPESILYRYL
                     TAPRMAAPRWYHEGLAVFVETWMAGGLGRAQGSYDEMVFRSKVRDGSRFYDPLGLVAE
                     GTKIDFQLEANSYLYGTRFITYLAYRYSPEVVLQWVTRAPGTRKYYAAQFERVFCRKI
                     EEVWQEWIEWERRFQQANLDTIREYPVTPHRDLSGRALGSVSRGFVDPATGDLLLAFN
                     YPGVVGHVGAIDTETGEVERLREVKGPMIYSVTSLAHDPEGRRLFYTADNTAFRDLYV
                     LELPDGKKKARKRRLMRDLRVGELVFNRADRSLWGVRHLNGMATLVRIPAPYDRWEQV
                     YTWRYGTVMYDLDVSPDGRLLSASVGNAAGKHSLRVMEIERVLEGDMTPLHEYEFGST
                     IPSGFVFSPDGRFLYGSSYYTGVSNIFRYELATRELEGLTNSETGFFRPIPLGDDRLY
                     VLRFTGGGFVPAEIEKAEPIEDVNAITFLGQLTVEQHPELETWNVGPPSEIDLDARIE
                     REGPFKPYRGIRLESVYPVIEGYKDFGAIGARMNFSDSVFLNRFDLSVSYTPDNDLPS
                     DERLHVEANYKRYDWRAYARWNRAEFYDLFGPTLVSRKGLGLGLGYNFTLVDDYPRKM
                     VLDLSATHAANGDQLPNAQNVLVDFQDLTTANVYWEFATKRASIAAVDYEKGIATGLN
                     ATGTYVDGRTIPSIYGWLDFGFPIPLKNSSVWFRNTAGRADGAVDDEFANFFFGGFGN
                     NYLDHREVKRYRDYYAFPGLALNNGFGRTFAKSMFEWNLPPVRFRDAGSPGFYATYAR
                     FSLFATALVTNPDLTELREEYGNAGFQMDVRLFVQSDLQMTFSVGAARAKQQGGTARD
                     EFMVSLKLLR"
     gene            complement(3567..>4443)
                     /locus_tag="VD788_16140"
     CDS             complement(3567..>4443)
                     /locus_tag="VD788_16140"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXV77841.1"
                     /translation="GLICLDSSLERAFREFDADLVSALGHHLAIAMERARLQALAIEK
                     ERLDRELAVARAIQVATLPSVVPAIPGYDLAGLSRPAEATGGDTYDLVPAGDDRRLIL
                     LLGDATGHGIGPALSVTQVRSMLRVAMRLEADLDSAFRHINDQLSEDLDDARFVTAFI
                     GMLDAVEHEVRYHSGGQGPIMHYHRKTERFDWHKATTTPMGFLPITEPPEACTIRLGP
                     GDILGLMTDGVFETESERGEMFGTTRVEALVRSHADKPADAIASAILEAIDDFAGATP
                     QADDITIVLVHREVS"
BASE COUNT          723 a         1506 c         1442 g          772 t
ORIGIN      
        1 ctcgccgcgg gcgacatgga tcgcgcgaag gccaacgccg atttcgcacg cgccatgggc
       61 aacgccgact tcgcacgcgc catgggcaac gccgatttcg cacgcgctat gggcaacgcc
      121 gacttcgcgc gtgccatggg caacgccgac ttcgctcgcg cgttgggcaa tgccgacttc
      181 gctcgcgcac tgggcaacgc cgacttcgct cgcgcgttgg gcaatgccga cttcgcacgc
      241 gcgttgggca acgccgactt cgctcgcgcg ttgggcaatg ccgacttcgc ccgcgcactg
      301 ggcaatgccg acttcgcccg cgcactgggc aacgccgact tcgcccgcgc gctggggaac
      361 cccgacttcg cacgtgcgat gagccactcg gacttcgcac gcgcgctggg gaacccgaac
      421 ttcgcccgcg cactcggtaa cgcggacttc aaccgcgccc tcgcgagcgg agacgtcgcg
      481 cagtttgccg cggactgacc gagtccgggt gttaccggag ctcggttcgg cgcgaaacga
      541 acgaatcgag gcaggagggg gatcgcggga acgcggtctc cctccttccg cgtctcgcta
      601 gggtctgcgc gtgcgcgcgc cgcgtgcgat cgtcttttgc tgggtcctgc tgctatctcc
      661 tttcgtccac gcgcagctga aggtcgtcga gacggagcac ctgcggctgg tctatttcga
      721 ccccgtccag gcgccgctcg ctccgtacgt cgcgcagtcc ttcgaaaact cgatggagtt
      781 tcagcgcgac ctgttcgact acgagccgtc cgagaaggtg acggtcttcc tgagcgacat
      841 cgcggactac ggcaacgccg gcgcgaccgc cgtgccgcgc aacttcgtcg ccgtgcaggt
      901 cgcccccaac agcttcgcct acgagaccta tccctcgaac gagcgcatca acacactgat
      961 gaaccacgag ctggtgcacg tcgccgcttt cgaccgaccc gccggcagcg accgtacctt
     1021 tcgcaagctc tttcggggca aggtctacga gaccgccgat catcccgagt cgatcctcta
     1081 ccgctacctg accgcgccga ggatggccgc gccgcgctgg taccacgaag ggctcgccgt
     1141 cttcgtcgag acctggatgg ccggcgggct gggccgggcc cagggctcgt acgacgagat
     1201 ggtcttccgc tcgaaggtgc gggacggaag ccgcttctac gatccgctcg gcctggtcgc
     1261 cgagggcacg aagatcgatt tccagctcga ggcgaactcc tatctgtacg gcacgcgctt
     1321 catcacctac ctcgcctacc gctactcgcc ggaggtggtg ctgcagtggg tcacccgcgc
     1381 tccggggacg cgcaagtact atgcggccca gttcgagcgc gtcttctgcc gcaagatcga
     1441 ggaggtctgg caggagtgga tcgagtggga gcgcaggttc cagcaggcga acctcgatac
     1501 gatccgcgag tatccggtga cgccccatcg cgatctgtcc ggtcgcgcgc tcgggtcggt
     1561 ctcccgcggt ttcgtcgacc cggcgacggg tgatttgctg ctcgcgttca actaccccgg
     1621 cgtcgtcggc cacgtcggcg ccatcgacac cgagacgggt gaggtcgagc ggctgcgcga
     1681 ggtgaagggg ccgatgatct acagcgtgac ctcgctcgcg cacgatcccg agggcagacg
     1741 cctgttctac accgccgaca acaccgcctt ccgcgatctc tacgtgctcg agctgccgga
     1801 cggcaagaag aaggcgcgca agagacggtt gatgagagac ctgcgggtcg gcgagctggt
     1861 cttcaaccgc gccgaccggt cgctgtgggg cgtgcggcat ctcaacggca tggcgacgtt
     1921 ggtccgtatc ccggcgccct acgatcgctg ggagcaggtc tacacctggc ggtacggaac
     1981 cgtgatgtac gacctcgacg tttcgcccga cggcaggctg ctgtcggcct cggtggggaa
     2041 cgcggccggc aagcacagcc tgcgcgtgat ggagatcgag cgcgtgctcg agggcgacat
     2101 gacgccgctc cacgaatacg agttcggcag cacgatcccg tccggcttcg tcttttcgcc
     2161 ggacggacgc ttcctctatg gcagctcgta ctacaccggc gtctccaaca tcttccgcta
     2221 cgagctggcc acgcgcgagc tcgagggcct gaccaactcg gagacgggct tctttcgacc
     2281 gatcccgttg ggggacgacc ggctgtacgt gctgcgtttc accggcgggg gtttcgtccc
     2341 ggccgagatc gagaaggccg agccgatcga agacgtcaac gcgatcacct tcctcggtca
     2401 gctgacggtc gaacagcacc cagagctcga gacgtggaac gtggggccgc ccagcgagat
     2461 cgatctcgac gcgaggatcg agcgcgaggg gccgttcaag ccctaccgcg gcatccggct
     2521 cgagtcggtc tacccggtga tcgagggata caaagacttc ggcgcgatcg gcgcgaggat
     2581 gaacttctcc gactcggtct tcctcaaccg cttcgatctc tcggtctcct acaccccgga
     2641 caacgatcta ccgtcggacg agcggctgca cgtcgaggcc aactacaagc gctacgactg
     2701 gcgtgcctac gcccgctgga accgcgccga gttctacgat ctgttcggac cgacgctcgt
     2761 cagccgcaag ggtctcggcc tgggcctggg ctacaacttc acgctggtcg acgattaccc
     2821 gcgcaagatg gtgctcgacc tcagcgcgac gcacgccgcc aacggcgacc agctgcccaa
     2881 tgcccagaac gtgctggtcg acttccagga cttgaccacg gccaacgtct actgggagtt
     2941 cgcgacgaaa cgcgcctcga tcgcggccgt cgactacgag aagggaatcg ctaccggcct
     3001 gaacgcgacg ggcacctacg tcgacggtcg aacgatcccc tcgatctacg gttggctcga
     3061 cttcggcttc ccgattccgc tgaagaactc ctccgtgtgg tttcgcaaca ccgccggccg
     3121 cgcggacggc gcggtcgacg acgagttcgc caacttcttc ttcggcggct ttggcaacaa
     3181 ctacctcgat caccgtgagg tgaagcggta tcgggactac tacgcctttc ccgggctcgc
     3241 gctcaacaac gggttcggcc ggaccttcgc caagtcgatg ttcgagtgga acctcccacc
     3301 ggtccgcttc cgcgatgccg ggagccccgg cttctacgcc acctacgcac ggttctcgct
     3361 cttcgcgacg gcgctcgtga cgaacccgga cctgaccgag ctgcgtgagg agtacggcaa
     3421 cgcgggcttc cagatggacg tgcggctgtt cgtgcagtcc gatctgcaga tgacgttctc
     3481 ggtcggcgcc gctcgcgcca agcagcaggg cgggacagcg cgcgacgagt tcatggtctc
     3541 gctgaagctg ctgcgctgat gacccgtcag gacacttccc tgtgtaccag tacgatcgtg
     3601 atgtcgtccg cttgcggcgt cgcgccggcg aagtcgtcga ttgcctccag gatcgcgctc
     3661 gcgatcgcgt cggccggctt gtcggcatga gagcggacca gcgcttcgac ccgcgtcgtg
     3721 ccgaacatct ctccgcgttc gctctcggtc tcgaacaccc cgtcggtcat cagtccgagg
     3781 atgtcccccg gtccgagccg aatcgtgcat gcctcgggag gctctgtgat cggcaggaag
     3841 cccatgggcg tcgtggtggc cttgtgccag tcgaagcgct ccgtcttccg atgatagtgc
     3901 atgatcgggc cctggccgcc cgagtggtag cgcacctcgt gctcgaccgc atcgagcata
     3961 ccgatgaaag ccgtgacgaa gcgggcgtcg tcgaggtctt cgctgagctg gtcgttgatg
     4021 tgccggaagg ccgagtcgag gtcggcctcg agccgcatcg cgacgcgcag catcgagcgg
     4081 acctgggtca cgctgagcgc cggaccgatg ccgtgcccgg tggcgtcacc cagcagcagg
     4141 atcaggcggc ggtcgtcgcc tgccggcacc aggtcgtagg tgtccccacc ggttgcctct
     4201 gccgggcggc tcagtccggc gaggtcgtag cccgggatcg cggggacgac cgacggcaac
     4261 gtggcgacct ggatcgcgcg agccacggcg agctctcgat ccagccgttc cttctcgatc
     4321 gcgagcgcct gcagtctcgc ccgctccatc gcgatggcga ggtggtgtcc gagtgcggag
     4381 acgaggtcgg cgtcgaattc gcgaaaggcc cgttcgagtg aggagtcgag gcagatcagg
     4441 ccc
//
LOCUS       DASPGN010000560         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_105410, whole genome shotgun sequence.
ACCESSION   DASPGN010000560 DASPGN010000000
VERSION     DASPGN010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105410"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..1106)
                     /locus_tag="VD788_16145"
     CDS             complement(237..1106)
                     /locus_tag="VD788_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77842.1"
                     /translation="MREPSPRRALALACLLGLLCGFGPSCRGPQDGSGEDVGGETPAA
                     AVAGNPPAAGFDLAGSDPQAIEVADSTMEAMGGRRAWDEARFLVWNFFGFRFHVWDKQ
                     TGDIRVEYATRDEPVQQVVVLMNLGTREGRAFVDGQPVADAEREAELLQHGYEAWVND
                     GYWLFMPYKLKDSGVTLSWLGERQMLDGRPADVLQLVFAGVGVTPENKYDVYVARDSG
                     LVEQWDFYADRADDEPRFQTPWHDWRPRGAIMLSGDRGQRQITEIAVYEELPPSVFDS
                     PEPTGLRQQDETS"
     gene            complement(1137..2507)
                     /locus_tag="VD788_16150"
     CDS             complement(1137..2507)
                     /locus_tag="VD788_16150"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV77843.1"
                     /translation="MNKILHVAVREFLATVATKGFVFGLVILPLVVLVAVFGIGLLID
                     EEAPRVEGDVAIFDPTGEVAEGLAAYLRPQAIAERREDFQELIDERLPSMPGAGTADA
                     RGVAMRTLLGEVPALEVVPLEAGADLEQAKRSLHEGPAEGGRLALVVVDDDAVVKPAD
                     SDRFGRYQLFVREKLDDRVVDEINDGLREAIVDARVGRAGLDRAYIDSLTRVGRVRSK
                     TVTAEGEKETNELLNAMMPAAFMLLLFASVMTGGQSLMTTTVEEKSSRVVEVLLSAVS
                     PMQLMTGKVIGQMLVGFLVLAVYGGMGVASLAFFALAGMVDLSLLFYLVIFYLIAYFV
                     VASLMAAIGAAVNEMREAQSLLGPVMLIVMIPWILWMPISRNPDSTFAVVTSFVPPLN
                     PFVMLIRMTSTSPPPSWQVWLSIAVGVGSVYGAVWLAAKVFRVGLLMYGKPPNFATLI
                     RWVRMA"
     gene            complement(2504..>3266)
                     /locus_tag="VD788_16155"
     CDS             complement(2504..>3266)
                     /locus_tag="VD788_16155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HXV77844.1"
                     /translation="RIGYLPEERGVYRKMKCGSFLTFIARLKGRDGPHLRQSVRQWLE
                     RVGLGDVENKRCEELSKGMQQKLQFIASVIHEPDLLILDEPFSGLDPVNMRLLRDLVI
                     EQKQRGATIIFSTHVMHQAERLCDHIVMINNGLKVLDDDLAAIRKRFDRKTILLEPLD
                     GPVDADRLRAIDGVAHVLHADGAYEVALTDDTDPARAMSRLIAAVPVARIELNRPTLE
                     EVFIRIVTEGRAADDEELHRLRASVTAGAAIGVDR"
BASE COUNT          559 a         1153 c         1058 g          496 t
ORIGIN      
        1 accgtggtgc ccgacgagcg ctggccggtt tcgtcgatga cgagcccctc gaggcgcgcc
       61 atcgggccgg cggcgaccac cgaccacgga acgaaacaga gcactagcgc cgcgatcgcc
      121 acgcgtgcgg tgagttcccg gagcgtctgg ttcatctccc ctccgcgtat gtcagagcgg
      181 cagtcgaccg aggcccagtg aaaccgatcg cccggggcga cgtcaagcgg attccgtcac
      241 gacgtctcgt cctgctggcg caacccggtc ggctcgggac tgtcgaagac ggacggcggc
      301 agttcctcgt acaccgcgat ctcggtgatc tgccgctggc cgcgatcgcc cgacagcatg
      361 atcgctccgc gcggtcgcca atcgtgccac ggggtctgga accgaggctc gtcgtccgct
      421 cggtcggcgt agaaatccca ctgctcgacc agacccgaat cgcgtgccac gtagacgtcg
      481 tacttgttct ccggcgtcac tccgacaccc gcgaagacga gttgcagcac gtcggccgga
      541 cggccgtcga gcatctggcg ctcgccgagc cagctcagcg tcacgccgct gtccttgagc
      601 ttgtagggca tgaacagcca gtagccgtcg ttgacccacg cttcgtatcc gtgctgcagc
      661 agttcggcct cgcgctcggc atccgccacc ggctggccgt cgacgaacgc ccggccctcg
      721 cgcgtgccga ggttcatcag cacgacgacc tgctgcaccg gctcgtcgcg cgtcgcgtac
      781 tcgacacgga tatcgccggt ctgcttgtcc cagacgtgga agcggaagcc gaagaagttc
      841 cagacgagga agcgcgcctc gtcccacgcc cgacggcccc ccatcgcttc catcgtcgaa
      901 tcggcgacct cgatcgcctg cggatccgag ccggcgagat cgaagccggc ggcgggcggg
      961 ttgccggcga ccgccgccgc cggcgtttca ccgccgacgt cctcccccga cccgtcctgc
     1021 ggcccgcggc aagacggtcc gaacccacag agcaacccga gcaggcaggc cagcgccaac
     1081 gcgcggcgag gcgacggttc tcgcatctcg ctctcctccg tcagggcagt tttcgatcag
     1141 gccatgcgga cccagcggat cagcgtggcg aagttcggcg gtttcccgta catcagcaga
     1201 ccgacgcgaa acaccttggc ggccagccac accgcgccgt agaccgatcc gacaccgacc
     1261 gcgatcgaca accagacctg ccacgacggg ggcggcgagg tcgaggtcat ccggatcagc
     1321 atcacgaacg ggttgagcgg gggaacgaag ctcgtcacca cggcgaaggt cgagtcggga
     1381 ttgcgcgaga tcggcatcca caggatccac gggatcatca cgatcagcat caccggaccg
     1441 agcaggctct gggcctcgcg catctcgttg accgccgcgc cgatcgcggc catcaacgag
     1501 gcgacgacga agtaggcgat caggtagaag atgacgaggt agaacagcaa cgacaggtcg
     1561 accatgccgg ccagcgcgaa gaacgccaac gacgccaccc ccatcccgcc gtagaccgcg
     1621 agcacgagaa acccgacgag catttggccg atcaccttgc ccgtcatcag ctgcatcggc
     1681 gagacggccg acagcaggac ttcgaccacg cggctcgatt tttcttcgac cgtggtcgtc
     1741 atcaacgatt gcccccccgt catcaccgag gcgaacagca gcagcatgaa cgcggccggc
     1801 atcatcgcgt tgagcaattc gttcgtttcc ttctcgccct cggccgtgac ggtcttcgag
     1861 cgcacccgtc cgacgcgggt cagcgagtcg atgtaggccc gatcgagccc ggcgcgtccg
     1921 acgcgcgcat cgacgatcgc ctcgcgcagg ccgtcgttga tctcgtcgac gacgcggtcg
     1981 tccagcttct cacgcacgaa cagctggtag cggccgaagc gatcggaatc ggccggcttg
     2041 accaccgcgt cgtcgtcgac gacgacgagc gccaggcggc cgccctcggc cgggccctcg
     2101 tgcagcgagc gcttggcctg ttcgaggtcg gcgcccgcct cgagcggaac gacctcgagc
     2161 gcgggcacct cgccgagcaa cgtgcgcatc gccacgccgc gggcgtccgc cgtgcccgcg
     2221 cccggcatcg acggcagccg ctcgtcgatc aactcctgga aatcctcgcg acgctcggcg
     2281 attgcctgcg gccggagata ggccgccagg ccttcggcca cttcaccggt cgggtcgaag
     2341 atcgcgacgt cgccttcgac gcgcggcgcc tcttcgtcga tcagcagacc gatgccgaag
     2401 acagcgacca ggacgacgag cggcaggatc accaggccga aaacgaagcc cttcgtcgcg
     2461 acggtggcga gaaattcgcg gacggcgacg tgcaggatct tgttcatcgg tcgaccccga
     2521 tggcggcacc ggcggtgacc gaggcgcgca gccgatgcag ctcttcgtcg tcggccgcgc
     2581 ggccctcggt gacgatccgg atgaacacct cctcgagcgt cgggcggttg agctcgatcc
     2641 gcgccacggg aaccgccgcg atcaggcgcg acatcgcccg cgcggggtcg gtgtcgtcgg
     2701 tcagcgccac ctcgtaggcg ccgtcggcgt ggagcacgtg cgcgacgccg tcgatggcgc
     2761 gcagccggtc ggcgtcgacc ggcccgtcga gcggttcgag caggatcgtc ttgcggtcga
     2821 accgcttgcg gatcgccgcc aggtcatcgt cgagcacctt gagcccgttg ttgatcatca
     2881 cgatgtgatc acacagccgc tcggcttggt gcatgacgtg cgtcgagaag atgatcgtcg
     2941 cgccgcgctg cttctgctcg atgacgaggt cgcgaagcag gcgcatgttg accgggtcga
     3001 gcccgctgaa cggctcgtcc aggatcaaca gatcgggctc gtggatcacc gacgcgatga
     3061 actgcagctt ctgctgcatc cccttcgaca gctcctcgca ccgcttgttc tcgacgtcgc
     3121 cgaggccgac gcgctcgagc cactggcgga cgctctggcg caggtgcgga ccgtcgcgac
     3181 ccttgaggcg ggcgatgaac gtcaggaacg agccgcattt catcttgcgg tagacgccgc
     3241 gttcttccgg caggtaaccg atccgg
//
LOCUS       DASPGN010000561         5436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_573788, whole genome shotgun sequence.
ACCESSION   DASPGN010000561 DASPGN010000000
VERSION     DASPGN010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573788"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VD788_16160"
     CDS             complement(<1..673)
                     /locus_tag="VD788_16160"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXV77845.1"
                     /translation="MMRTGTIVTLLCLLVLGSACSSGDRAPEVAKPRAVVLITVDGML
                     GADLALFGGATPTPGLARLAARGAAWDDAWTAAPMTRPAVATYLTGLAPDRHRVRDDL
                     FTGLDGGVPTLATAFAAAGYDTAAFPDSSFLGYGCGLLDGFEVAAAPPALRIGGERWV
                     PDIRTVDAITADFDAWLDSVAAERPYFAWLHFSGPMMAQIWEATTSRITSPEVAARRV
                     AAREEQ"
     gene            complement(789..1697)
                     /locus_tag="VD788_16165"
     CDS             complement(789..1697)
                     /locus_tag="VD788_16165"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV77846.1"
                     /translation="MQFVRTRVGAVVTVLALSLAMALGAVAFEVHLRTRPPRVVAAPV
                     DFESMMLQVEEVEFPATDDVLLSGWLIRGNPALAPIVLCHDFGSDRHAVLNTALSLSH
                     AGFTVLAFDFRGHGASGGDRSTLGLREKRDVLGAVDYLLATGPNDERRIGVFGAGMGA
                     YAAVLAAADRPSIKVLVLDGLYPDVGFPLSRAIFHDWNFAVDHLGFLPRGWFLLLHGA
                     AVGEERAADVIPALVGRDMLLLAPAGDTRLADAMMGMYESVPEQADADGNLVVVPVAH
                     GDGLYADQLARYHDRVTGFFLSRLDG"
     gene            complement(1809..3605)
                     /gene="uvrC"
                     /locus_tag="VD788_16170"
     CDS             complement(1809..3605)
                     /gene="uvrC"
                     /locus_tag="VD788_16170"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HXV77847.1"
                     /translation="MNETLRRKLETLPAAPGVYRYRNADGEVIYVGKAKSLRQRVRSY
                     FQPSAQHPPRVDRMISEVDDLELIVVGTEMEALILESNLIKRERPRYNVVLRDDKHFP
                     YLKLSLKDTYPRVSLVRRARRDGQLYSGPFLPASTARRSMKLIQKFFQVATCNEVFDG
                     RRRPCLYYHLDQCLAPCAGKTDPAEYGAAVDHARLFLEGRDHDLIASLEERMRAAAGE
                     QAYERAARLRDTLRIVRGLSVRQRMSSVGLEEQDYLAHHAEGRQVALQLFQIRQGKVQ
                     ARREFTFDEIDFEPREFYGSVLTQYYDDTVPPSEIYLADAPGDQPLLEGWLTGRRGAA
                     VRLKVPRRGPKKKLIDLVRDNARLAFDSRFRARHGHGVEAAEQLARVLGLDEAPTRIE
                     CFDISNIHGTDSVASMVVWDSGRALRSQYRNFNIKTVEGADDFASIAEAVSRRYRRLV
                     AEDRKLPDLVLIDGGRGQLGAAVSALARVGLPMLPVVSIAKREEELFLETRAEPVRLA
                     RDSKALQLVQRIRDEAHRFAVTRHRARRSRRTLKTELTELPGVGPATAKKLLREFGSV
                     DGVRRAGYESWRRVAGARVADSLASRYGRPAG"
     gene            complement(3602..4468)
                     /locus_tag="VD788_16175"
     CDS             complement(3602..4468)
                     /locus_tag="VD788_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77848.1"
                     /translation="MTRSRTTFDLSGVVVALDGLPAEAGARLLAQWSAFLADDRPPFL
                     ELAVRLATRPAPPSAFAPKRMRSALAATHARFEMPEGLAEVDARGRGRIELTRGLGTR
                     EFFTLLNLLRACLAWRLPDRGAAMLHAAGLVIDGRGFLLVGAEGAGKSTWARLGETVG
                     AHVVSDDLVLVDPEADGYALLGAPFRSTHPVTYRPGRWPLAAILFPRHGTAPRLQPTP
                     GLIARARLTANLTFVADAPAADGRIPRVVDRLARVVRCEELTFALDAGFVELLRGIDR
                     PPDGRDPAGART"
     gene            complement(4470..5372)
                     /locus_tag="VD788_16180"
     CDS             complement(4470..5372)
                     /locus_tag="VD788_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77849.1"
                     /translation="MSPRPSEPRPPSYFDVERAAVGSRAMTLVLGDLAIELRGVDSPL
                     ERRLAGRYGPYLGPADRPALCVDVAHETGFDYFIEPRPGESNPLLLACDGPRVRTTGY
                     CSAGWFDTRGGCGQLVLSDGRHETRERSIENYLRAATAWVAAERGGALIHAASSVLDE
                     RGYLFYGESGAGKSTLSAHNRRARVVSDDLSLVLPGEDGGPWLVGTPFRGTYEEGEPF
                     LGRARLVAGFRIVKDAHAEVRAVPRVRAFAELVGNLPFVAEAFSRRPDLFERVDRAFR
                     DVPLAHLHFRKDDDYWDAIAAAGL"
BASE COUNT          861 a         1931 c         1941 g          703 t
ORIGIN      
        1 tttgctcctc gcgggccgcg acgcgcctcg ccgcgacctc gggcgaggtg atgcgcgacg
       61 tcgtcgcttc ccagatttgc gccatcatcg gcccactgaa gtgcagccag gcgaagtagg
      121 ggcgttcggc ggccaccgaa tcgagccacg cgtcgaagtc cgcggtgatc gcgtcgacgg
      181 ttcggatatc gggaacccag cgttccccgc cgatgcggag cgccggcggc gcggcggcga
      241 cctcgaaacc gtcgagcagc ccgcagccgt aacctagaaa gctcgaatcg gggaaggccg
      301 cggtgtcgta tcccgccgcg gcgaacgccg tggccagcgt cgggacgccg ccgtcgaggc
      361 cggtgaacag gtcgtcgcgt acgcgatgcc gatcgggcgc gaggccggtc aggtaggtcg
      421 ccaccgccgg gcgcgtcatc ggcgcggcgg tccaggcgtc atcccacgcc gcaccgcgcg
      481 cggcgagccg tgcgaggccc ggcgtcggcg tcgccccacc gaacagcgcg agatcggccc
      541 ccagcatccc gtcgaccgtg atcagaacga ccgcacgcgg cttcgcgacc tcgggtgcgc
      601 ggtcgccgct cgaacacgcc gagccgagca cgaggagaca aagcagggtg acgatcgtac
      661 cggttcgcat catcgctcgc accctcgaca agtcggacgg atcgttatgc gtgaagtgca
      721 gaagatagcc caatcgacgg acccgttcca atcgcggcgg acggccgagg cgactcgatc
      781 cgacgccgtc agccgtcgag gcgactcagg aagaacccgg tgacgcgatc gtggtagcgg
      841 gccagctggt cggcgtaaag accgtcgccg tgcgcgaccg gcacgacgac caagttgccg
      901 tcggcgtcgg cctgctccgg aaccgattcg tacatgccca tcatcgcgtc ggccaggcgg
      961 gtgtcccccg ccggggccaa cagcagcatg tcgcgcccga cgagcgccgg aatcacgtcg
     1021 gcggcccgct cttcgccgac ggcggcgccg tggagcagca ggaaccagcc gcgcgggagg
     1081 aagccgaggt ggtcgaccgc gaagttccag tcgtggaaga tcgcgcgcga cagcgggaag
     1141 ccgacgtcgg gatagaggcc gtcgagcacc agcaccttga tcgacgggcg atccgccgcc
     1201 gccagcaccg cggcgtacgc tcccatgccc gcgccgaaca caccgatccg ccgctcgtcg
     1261 ttcggccccg tggccagcag gtagtcgacc gcgcccagca cgtcgcgctt ctcgcgaaga
     1321 ccgagagtcg agcgatcgcc gccgctcgcg ccatgaccgc gaaaatcgaa ggcgagcacg
     1381 gtgaacccgg cgtggctgag cgatagggcg gtgttgagca ccgcgtgccg gtcggaaccg
     1441 aagtcgtgac acaggacgat cggcgcgagc gccggattgc cgcggatcaa ccagccggag
     1501 aggaggacgt cgtccgtcgc tggaaactcg acctcttcga cctgcagcat catcgactcg
     1561 aaatcgacgg gagcggcgac gacgcgcgga ggccgcgttc ggagatgcac ctcgaacgcg
     1621 acggcgccga gcgccatcgc gagcgacagc gcgagcacgg tcacgacggc gccgactcgc
     1681 gtccgaacga actgcatggt accccttcgc ctgcgacgcc tgccgtgcgg caggccccgg
     1741 acagggctcc gccgacgggc cacacctcgc ggtgccgaat ataggttgac cgacgagccc
     1801 gggccacgtc agccggctgg acgcccgtag cgcgacgcga gcgagtcggc gacgcgggcg
     1861 ccggcgaccc gacgccagga ttcgtagccg gcgcgccgga ccccgtcgac gctgccgaac
     1921 tcgcgcagca gcttcttcgc ggtcgccgga ccgactccgg gcagctcggt cagctcggtc
     1981 ttgagcgttc ggcgcgagcg acgggcacgg tgccgggtca cggcgaagcg gtgggcctcg
     2041 tcgcggatcc gctggaccag ctgcagcgcc ttggagtcgc gcgcgagacg caccggctcg
     2101 gctcgcgtct cgaggaacag ctcctcctcg cgcttcgcga tcgacaccac cggcagcatc
     2161 ggcagaccga cgcgggcaag cgcggaaacc gcggcgccga gctggccgcg tccgccgtcg
     2221 atcagcacca ggtcgggcag cttgcgatct tcggcgacga gccgccgata ccgtcgcgag
     2281 accgcctcgg cgatcgaggc gaagtcgtcg gcgccttcga ccgtcttgat gttgaagttg
     2341 cgatactggg agcgcagtgc gcgcccgctg tcccacacga ccatcgacgc caccgagtcg
     2401 gtgccgtgga tgttcgagat gtcgaagcac tcgatgcggg tcggtgcctc gtcgagaccg
     2461 aggacccgcg ccagctgctc cgcggcctcg acaccgtggc cgtgccgcgc acgaaagcgg
     2521 ctgtcgaacg ccagccgggc gttgtcccgg acgagatcga tcagcttctt tttcggaccg
     2581 cgccgtggca ccttcaaccg aacggcggcg ccgcggcggc cggtgagcca gccctcgagc
     2641 agcggctggt cgcccggtgc gtcggcgagg tagatctcgg acggcggcac cgtatcgtcg
     2701 taatactggg tcaacaccga gccgtagaac tcacgcggct cgaaatcgat ctcgtcgaag
     2761 gtgaactcgc ggcgggcctg caccttgccc tggcgaatct ggaacagctg gagcgcgacc
     2821 tgccggccct cggcgtgatg cgcgaggtag tcctgctcct cgagtccgac cgaggacatc
     2881 cgctggcgga ccgacagccc ccgcacgatc cgcagcgtgt cgcgcaggcg cgcggcacgc
     2941 tcgtaggcct gctcgccggc ggccgcgcgc atccgttcct cgagcgacgc gatcaggtcg
     3001 tggtcgcggc cctcgagaaa caaccgagcg tgatcgaccg ccgcaccgta ctccgccggg
     3061 tcggtcttgc cggcgcacgg ggcgaggcac tgatcgaggt ggtagtagag acagggacgc
     3121 cgtctgccgt cgaacacctc gttgcacgtc gccacctgga agaacttctg gatcagcttc
     3181 atcgagcggc gtgccgtcga ggccggcagg aacggtccgc tgtagagctg gccgtcgcgt
     3241 cgcgcgcggc gaacgagaga cacccggggg taggtgtcct tcagcgagag cttgagatag
     3301 ggaaagtgct tgtcgtcgcg cagcacgacg ttgtagcgcg ggcgctcgcg tttgatcagg
     3361 ttcgactcga gaatcagcgc ttccatttcg gtcccgacga cgatcagctc gagatcgtcg
     3421 acctcggaga tcatgcggtc gacgcggggc gggtgctggg ccgacggctg gaagtacgag
     3481 cgcacgcgct ggcgcagcga tttcgccttg ccgacgtaga tcacctcgcc gtcggcgttg
     3541 cggtagcggt agacaccggg cgccgcgggc agcgtctcga gcttgcggcg cagcgtctcg
     3601 ttcacgtcct ggccccggcc gggtcgcggc cgtcgggcgg ccggtcgatc ccgcgcagca
     3661 gctcgacgaa gccggcgtcg agcgcgaagg tcagctcctc gcagcgcacc acgcgggcca
     3721 gccgatcgac cacgcgcggg atgcgcccgt cggccgccgg cgcgtcggcg acgaaggtga
     3781 ggttggccgt caatctcgcc cgtgcgatca gcccgggggt cggctgcagc cgcggcgccg
     3841 tcccgtgtcg cgggaacagg atcgcggcca gcggccagcg gcccggccgg taggtcaccg
     3901 ggtgggtcga gcggaacggc gcaccgagca gagcgtagcc gtccgcctcg gggtcgacca
     3961 gcaccagatc gtcactgacg acgtgtgccc cgaccgtctc gcccaggcgg gcccacgtgc
     4021 tcttgcccgc cccttccgcg ccgaccagca ggaacccgcg tccgtcgatc accagccccg
     4081 ccgcatgcag catggccgcg ccgcgatcgg gcagacgcca agccaggcag gcgcgcagca
     4141 ggttcaacag cgtgaagaac tcgcgagtgc cgagcccacg ggtcaactcg atgcgaccac
     4201 gcccccgcgc gtcgacctcg gcgagcccct cgggcatctc gaagcgtgcg tgggtcgcgg
     4261 cgagcgccga gcgcatccgc ttcggcgcga aggccgacgg cggcgcgggc cgcgtggcga
     4321 gccggacggc gagctcgagg aacggcgggc ggtcgtcggc gaggaacgcc gaccactgtg
     4381 cgagcaggcg ggcccccgcc tccgcgggca ggccgtcgag cgcgacgacg accccggaca
     4441 gatcgaaggt cgtgcgcgat cgcgtcacct cacagccccg ccgccgcgat cgcgtcccag
     4501 tagtcgtcgt ccttgcggaa gtggaggtgc gcgagcggga cgtcgcggaa cgcccggtcg
     4561 acccgctcga acagatccgg tcgccgggag aaggcctcgg cgacgaacgg caggttgccg
     4621 accagctcgg cgaaggctcg tacgcgcggg accgcgcgca cctccgcgtg cgcgtccttg
     4681 acgatgcgga agccggccac cagccgcgcg cgaccgagga acggctcgcc ctcctcgtac
     4741 gtgccgcgga acggcgtgcc gacgagccac gggccgccgt cctcgcccgg cagcacgagc
     4801 gacaggtcgt cgctgacgac acgcgcccga cggttgtgcg ccgacagcgt cgacttgccc
     4861 gctcccgact cgccgtagaa cagataaccg cgctcgtcga ggaccgacga ggcggcatgg
     4921 atcagtgccc cgccacgctc ggcggcgacc caggcggtcg ccgcccgcag gtagttctcg
     4981 atcgagcgct cccgcgtttc gtgccgcccg tcggataaca ccagctgccc acacccgccg
     5041 cgcgtgtcga accagccggc cgagcaatag ccggtcgttc gcacgcgcgg gccgtcgcac
     5101 gccagcagga gcgggttgct ctcgcccggt cgcggctcga tgaagtagtc gaagccggtc
     5161 tcgtgggcga cgtcgacgca cagcgccggc cggtcggccg gaccgaggta gggaccgtag
     5221 cggccggcca ggcggcgctc gagcggcgag tcgaccccgc gcagctcgat cgcgaggtcg
     5281 cccaggacga gcgtcatcgc tcggctgccg actgcggcgc gctcgacgtc gaagtaggag
     5341 ggcggccgcg gttcgctcgg gcgcgggctc atgccagcac cgccttctcg cgcaggtagc
     5401 ggatccggtc gcggtactcc gcggcgcgct cgaact
//
LOCUS       DASPGN010000562         6821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1745802, whole genome shotgun sequence.
ACCESSION   DASPGN010000562 DASPGN010000000
VERSION     DASPGN010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6821
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1745802"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VD788_16185"
     CDS             <1..849
                     /locus_tag="VD788_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77850.1"
                     /translation="SPVAGSFLGAGLFLSYAAYIAAVVGLHRLVGADFPTRIAERAVV
                     YLSIFPVAYFLHAAYTESLFLALVVWSFVFARRRSWALAGLCGGLASMTRITAFGLLP
                     AIAFEYLLDKRFELRRIRPDVLWLLMIPIGFVVYLWVNHSVFGSPWAFLEVNRLEAHK
                     VLAPPWVGARTVWNSGLSYGPSRFLLVGISEVVAGVLSGLLTIYVAFRLRGSYAIYMT
                     VAWLTFASTSFWASTTRYVLPLFPGFIVLAVWGERRLAHWAMTVAFLMGYSLLLARFV
                     RGSWAF"
     gene            837..2174
                     /locus_tag="VD788_16190"
     CDS             837..2174
                     /locus_tag="VD788_16190"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HXV77851.1"
                     /translation="MGVLTANGPPRRWSEAFRNDLVPGLLFLLAMLLYAPRLDVPPRY
                     MFDEVYHSFTAGQYVAGNADAYVWYTDAPRQDVAYMWNHPPAGVLMIAGGILLWGDHP
                     FGWRFSSAVFGAAGVLLVYLLALRMTRDRWVAVLSAFLVLCDTLWFVQSRVGMLDVFG
                     AVFATAALFSLYGYLTAPPERIVRSLAWTGLFLGLALTTKWNAAFVSVCCGSLVLYRC
                     FRSWLAARFDPTPRTRQVWHAHRIGLPLGFAVIPALVYLVGYVPFFATGHDWSQFVEL
                     QRQIFVYHSRLEATHGYQSLWWEWPLALKPVWYSVTYTPATVAHVYANGSPLLYWGFI
                     PSVLAVSAGWWRRSDPALAVLLIGFFGQWLPWALSPRTAFAYHFLPAVPFGCIAVACV
                     GLGLWRRGRLWRSVVAVYLLLLVAYFFFAYPIHAALPVPRQSFIVRHFVPSWK"
     gene            complement(2140..4053)
                     /locus_tag="VD788_16195"
     CDS             complement(2140..4053)
                     /locus_tag="VD788_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77852.1"
                     /translation="MILYPALALALLGAMGALGIGLLARTAPWLDRLERWVYGATLGV
                     VIGSLALLLLAWPLGLTVRTVWLVAVGCAAGAVFLLRGGMGRTEPEASSPGRFAVVVL
                     LVLVVRLGLFWSGAVRVESDGMWVAYTGLWGDWAQHLGDVTAFVYGDNFPPGQTRFAG
                     SPLAYHYLTSVTAAAMVVAGLTPWSALALQSFVFSVIVVLSLFVLARRFTGDTSAAAL
                     VALLFLVGGGLGWTLTLEAALGPGGSANVLLERPWDTGRLDAENYQWLNVFFSSIAPQ
                     RAYLYGIPLYGLILTSLSHAVRTRALRWFALAGAVAGLLPFAHLGAMLCLALVTPIVA
                     VQVPMRGWVGFFAVWTAVGLPQVVVQQGGTAGVLASFRWAPGWVSAPDLWIWFWLKNL
                     GLFLPLLVWALGETSLIDRISRRMLWAFMPLFVAANLFVFQPWDWDNTKLLVFWYLAA
                     CVFVAALLVRVWRRFPAVAARGTWVLIVASLLLSGVLQNFHQLLGLDRNRLLTRDELT
                     LADRVREATDPHARFLTGQQHNHPIHVMAGRAVVLGYPGWLWSQGYDYRARQQDVRSM
                     YALDGDAVRLLEHYAVDYVVVGPWEREKLAGDPEAFRSRYPSVIRTESYEVFAVGRTG
                     DRATSTKERSGGR"
     gene            4152..5537
                     /locus_tag="VD788_16200"
     CDS             4152..5537
                     /locus_tag="VD788_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77853.1"
                     /translation="MITPRHVGKTAVAAVPLLVSCAAATVAGEVFRRAEALEEIVGRV
                     EVRRSFGAALEVVVECGERPAGCREERTYGDLIELEFWLRRQETRDSSVTSSEDPAGG
                     ILLAADFEDADRESFLAELEAALTALPATAFDLPLAGLGPSRPPFDKGTLAHRSFEPT
                     WVCFRTGSGTKVVRYHRRNDGDEVVGVGAKHYGNAPTDLCDQIRTRTRTAYPESFWTL
                     SSPRRLLERVVDHEEVVRLGDDPRSLTDRPDGVQLVKQKMTIDAAIDSAAVDEGGLSR
                     ATHASNGWRFDAWAPPAAMPVAALDENAWLQAWRIRSVEREFGADPESRVVGDITLYA
                     AFLATLPAARLASPAADGSYRVSSTGHLESELVVAAAGSAPGIDRGVFVEQVIADLEA
                     NAGDPRDPIERMLLRPFVGLTSEGWIAVVCSPDGSASYSLPPTRASNAVTNADIFCGA
                     LESRLPGPGLE"
     gene            5582..6385
                     /locus_tag="VD788_16205"
     CDS             5582..6385
                     /locus_tag="VD788_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77854.1"
                     /translation="MTFRASILRKGRVSLRQQFRYVKGDVMARSRLTTNVALIALAIG
                     SLIAIQALTGTDGVSFLNAAERGSERGVRKQAPPRSQNPPLEAATRGSRGNGNGNGNV
                     CQGDVKIDMGVQDGDHEYDAPAGESISSICIKAGQGQFNFECGQTDETGCYDVTWTEA
                     SPGCCTSVTIGGGGTSSECKDISHTTATFGGPPCASPEPSPEPSPEPSPEPSPEPSPE
                     PSPEPSPEPSPEPSPEPSPEPSPEPSPEPSPEPSPEPSPEPSPEPSPEP"
     gene            complement(6417..>6821)
                     /locus_tag="VD788_16210"
     CDS             complement(6417..>6821)
                     /locus_tag="VD788_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77855.1"
                     /translation="VRLRDGRVEVRSAERNWTVGQGAELRVRPDGTETHGAIPSHGVE
                     WDWTLGVTPVPDFGGEPMSAFLAWVARERGWSIAYADAMTERSADSSILRGSLALLRV
                     DEALEAAMTATGLRYRIDRGTMVVSRTDPERR"
BASE COUNT         1055 a         2353 c         2328 g         1085 t
ORIGIN      
        1 agcccggtcg ccggtagctt cctcggggcg ggcctgttcc tgtcctacgc ggcctacatc
       61 gcggccgtgg tcggtttgca ccgtctggtc ggtgccgact tcccgacgcg gatcgccgag
      121 cgggcggtcg tctacctgtc gatcttcccg gtcgcctatt tcctgcacgc ggcctacacc
      181 gagtcgctgt tcttggcact cgtcgtctgg agcttcgtct tcgcgcgccg gcgcagctgg
      241 gccctggccg ggctgtgcgg cggtctcgcg agcatgaccc gcatcaccgc gttcggcctg
      301 ctgccggcga tcgccttcga gtacctgctc gacaagcggt tcgagctgcg gagaatacga
      361 cccgacgtgc tgtggttgct gatgatcccg atcggcttcg tcgtctacct gtgggtgaat
      421 cactcggtct tcggcagccc gtgggcgttc ctcgaggtga atcgcctgga ggcccacaag
      481 gtgctcgcgc cgccgtgggt cggcgcccgg accgtctgga actcggggct cagctacgga
      541 ccgagccggt ttctcctggt cgggatcagt gaggtcgtcg ccggcgtgct ctcggggttg
      601 ttgacgatct acgtcgcgtt ccgcctccgc ggctcctacg cgatctacat gacggtggcc
      661 tggttgacct tcgcatcgac ctcgttctgg gcgtcgacca cgcgctacgt gctgccgttg
      721 ttcccgggat tcatcgtgct cgccgtttgg ggcgagcggc gcctcgctca ctgggcgatg
      781 acggtggcgt tcctgatggg atacagcctg ttgctcgcgc gcttcgtgcg cggttcgtgg
      841 gcgttttgac ggcgaacgga ccaccgcgcc gctggtcgga ggcgttccga aacgacctcg
      901 ttccgggcct gctcttcctg ttggcgatgc tgctgtacgc tccgcgactc gacgtgccgc
      961 cgcgctacat gttcgacgag gtctatcaca gcttcaccgc gggtcaatac gtggcgggca
     1021 acgcggacgc ctacgtctgg tacaccgacg cgccgcgcca ggacgtggcg tacatgtgga
     1081 accacccgcc cgccggggtg ctgatgatcg ccggcgggat cctgctgtgg ggcgaccacc
     1141 cgttcggctg gcgtttctcg agcgcggttt tcggggccgc cggcgtgctg ctcgtctacc
     1201 tgctggcgct gcggatgacg cgcgaccgct gggtcgccgt gctgtcggcg ttcctcgtgc
     1261 tgtgcgatac gctgtggttc gttcagtcgc gggtcggcat gctcgacgtg ttcggcgccg
     1321 tgttcgcgac cgccgcgttg ttctcgctct acggctactt gaccgcgcca cccgagcgca
     1381 tcgtccgatc gttggcctgg accggtctgt tcctgggttt ggcgctgacg accaagtgga
     1441 acgcggcctt cgtctcggtc tgttgcggct cgctcgtgct ctaccgctgc tttcgcagct
     1501 ggctcgcggc gcggttcgac ccgacgccgc ggacgcgcca ggtgtggcac gcacaccgga
     1561 tcgggctgcc gttgggcttc gcggtgatcc cggcgctcgt ctacctggtg ggctacgttc
     1621 cgtttttcgc caccggccac gactggtcgc agttcgtcga gctgcagcgg cagatcttcg
     1681 tctaccactc gagactcgag gcgacgcacg gctaccagtc gctgtggtgg gaatggccgc
     1741 tcgcgctgaa accggtgtgg tattcggtga cctacacgcc ggccacggtg gctcacgttt
     1801 acgccaacgg aagcccgttg ctctactggg gcttcatccc gtcggtgctc gcggtttccg
     1861 ccggatggtg gcgaaggtcc gatccggcgc tcgccgtgct gctgatcggc ttcttcggcc
     1921 agtggctgcc ctgggcgctg tcgccgcgga ccgcgttcgc ctatcacttc ctccccgccg
     1981 tgcccttcgg ctgcatcgcg gtcgcgtgcg tcggccttgg cctgtggcgt cgcggtcgtc
     2041 tctggcgatc ggtcgtggcg gtttacctgc tgctgctcgt cgcgtatttc ttcttcgcct
     2101 acccgatcca cgccgccctg cccgtcccac ggcagtcgtt catcgtccgc cacttcgttc
     2161 cttcgtggaa gtagctcgat cgccggtccg accgacggcg aacacctcgt acgattcggt
     2221 ccggatgacg gacgggtaac gcgagcggaa cgcctcggga tcgccggcga gcttctcccg
     2281 ctcccacggg ccgacgacga cgtagtccac ggcgtaatgc tcgagcagcc gcaccgcgtc
     2341 gccgtcgagc gcgtacatcg acctgacgtc ctgctggcgc gcgcgatagt cgtagccctg
     2401 gctccacagc cagcctggat accccagcac gaccgcccgc ccggccatca cgtggatcgg
     2461 gtggttgtgc tgctgcccgg tgaggaaccg cgcgtgcggg tcggtcgctt cgcgcaccct
     2521 gtcggccaag gtcagctcgt cgcgcgtcag caggcggttc cggtcgaggc cgagcagctg
     2581 atggaagttc tgcagcacgc ccgacagcaa caagctggcg acgatcagga cccacgtgcc
     2641 ccgggcggcg accgcgggga accgacgcca gacccgcacg agcagcgcgg cgacgaatac
     2701 gcaggccgcg aggtaccaga aaacgagcag cttggtgttg tcccagtccc agggttggaa
     2761 gacgaacaga ttggccgcga cgaacagggg catgaaggcc cagagcatgc ggcgcgagat
     2821 gcgatcgatc agcgaggttt ctcccagcgc ccagaccagg agcgggagga acaggccgag
     2881 gttcttgagc cagaaccaga tccaaaggtc gggcgccgag acccagcccg gcgcccaccg
     2941 aaacgaggcc agcacgccgg cggtgccgcc ctgctggacc acgacttgcg gcaggccgac
     3001 cgcggtccac accgcgaaga acccgaccca gccccgcatc gggacctgga cggcgacgat
     3061 cggggtcacg agcgcgagac acagcatcgc gccgagatgg gcgaacggga gcagcccggc
     3121 caccgcaccg gcaagcgcga accagcgaag cgcccgcgtt cgaaccgcgt gcgacaagga
     3181 ggtcaggatc agaccgtaga gcggaatccc gtagaggtag gctcgctggg gcgcgatcga
     3241 cgagaagaag acgttgagcc actggtagtt ttcggcgtcc agccggcccg tgtcccacgg
     3301 gcgctcgagc agcacgttcg ccgatccccc cgggccgagc gccgcctcga gggtcagcgt
     3361 ccaaccgagg ccgccgccga cgagaaagag gagggcgacg agcgccgccg cgctcgtgtc
     3421 gccggtgaat cgccgcgcca gcacgaacag cgacaggacg acgatcaccg agaagacgaa
     3481 gctctggagg gcgagcgccg accacggggt gaggccggcg acgaccatcg ccgccgccgt
     3541 gaccgaggtc aggtagtgat aggccagcgg cgatccggcg aatcgcgttt gccccggcgg
     3601 gaagttgtcg ccgtagacga aggcggtcac gtccccgagg tgctgggccc aatcgcccca
     3661 cagaccggta tacgccaccc acatgccgtc ggactcgacc cgaacggctc cactccagaa
     3721 cagcccgaga cgaaccacga ggacgaggag gacgacgacg gcgaagcgac cgggactcga
     3781 cgcttccggt tcggtgcggc ccatcccgcc gcgcagcaag aagacggcgc ctgccgcgca
     3841 gcccaccgcc acgagccaga ccgtgcgtac ggtcaagccg agcggccagg cgagcaggag
     3901 caatgcgagc gagccgatga cgacgccgag cgtggcgccg tagacccagc gctcgagccg
     3961 gtcgagccac ggggcggtgc gagcgagcag cccgatcccc agagcgccca tcgctcccaa
     4021 cagggccaga gcaagagccg ggtacaggat caaaccggac cttcgatggc gttcgacgca
     4081 ggccggattc tacgtcatcg atcgttcgag gggggcggcg ggatggcagc acccaccgta
     4141 tgcttggaac cgtgatcacg ccgagacatg tcgggaagac ggccgtcgcg gcggtcccgc
     4201 ttctcgtctc gtgcgcggca gcgaccgtcg ccggcgaagt gttccgccgc gcggaggcgc
     4261 tcgaggagat cgtcggcagg gtggaagtcc gtcgcagttt cggtgccgcc ctcgaggtcg
     4321 ttgtcgaatg cggggagcgg cccgccggct gtcgcgagga gcggacctac ggcgacctga
     4381 tcgagctcga gttctggctg cggcgccagg agacgcggga cagctcggtg acgagctcgg
     4441 aggaccccgc cggcgggatc ctcctggccg ccgacttcga ggacgccgat cgcgagtcgt
     4501 tcctcgccga attggaggcc gcgctgacgg cgctgcccgc cacggcgttc gatctcccgc
     4561 tggcgggtct cggcccgtcc cggccaccgt tcgacaaggg cacgctcgcc caccgctcgt
     4621 tcgagccgac gtgggtctgc tttcggaccg gaagcgggac gaaggtcgtc cgctaccacc
     4681 ggcgcaacga cggcgacgag gtcgtcggcg tcggtgcgaa gcactacgga aacgcaccga
     4741 ccgatctctg cgaccagatt cgtacccgaa cgcgcaccgc ctatcccgag tcgttctgga
     4801 ccctgtcgtc gccgcgtcgt ctgctggagc gtgtcgtcga tcacgaggag gtggtccgac
     4861 tcggagacga tccacggtcg ctcaccgacc gtcccgacgg cgttcaactc gtgaagcaga
     4921 agatgacgat cgacgctgcg atcgactctg cggccgtcga cgagggcggt ctttctcggg
     4981 cgacccacgc atcgaacggg tggcgtttcg acgcctgggc cccgccggcc gcgatgcccg
     5041 tcgcggcgct cgacgagaac gcgtggttgc aggcgtggcg catccgcagc gtcgaacgcg
     5101 agttcggcgc cgatcccgaa tcccgcgtgg tcggcgacat cacgctctat gcggcgttcc
     5161 tcgccaccct tccggccgca cggctcgcct cgccggctgc cgacgggtcg tatcgagtct
     5221 cgtcgacggg ccatctcgag tcggagctcg tggtggcggc ggccggttcc gcaccgggga
     5281 tcgaccgggg cgtgttcgtc gaacaggtga tcgcggatct cgaagcgaac gccggtgacc
     5341 cgcgcgaccc gatcgagcgg atgctgctgc ggccgttcgt cggcctgacg agcgagggct
     5401 ggatcgccgt ggtctgcagc cccgacggct cggcgagcta ctcgctgccg ccgacccgcg
     5461 cctcgaacgc ggtcacgaac gcggacatct tctgcggggc gctcgagagc cgcttgccgg
     5521 gacccggact tgaataattg atggtttatc gtgtgaaact cgacacccct gcagccgaat
     5581 tgtgacgttt cgagcttcca ttcttcgcaa agggcgcgta tcgttacggc aacaattcag
     5641 atacgtgaag ggggatgtca tggcgcgctc gcggctcacg acgaatgttg cactgattgc
     5701 actggcgatc ggttccctaa tcgccatcca ggcgctgacc ggcacggacg gggtttcctt
     5761 cctcaacgcc gccgagaggg gatccgagcg cggcgtacgc aagcaggccc cgccacgcag
     5821 ccagaaccca ccgctcgaag ctgccacgcg cggctcccgg ggcaacggca atggcaatgg
     5881 caacgtctgt cagggagacg tcaagatcga catgggcgtc caggacggcg accacgaata
     5941 cgacgcgccc gccggcgagt cgatctcctc gatctgcatc aaggccggac aggggcagtt
     6001 caacttcgag tgcggccaga ccgacgagac cggctgctac gacgtcacct ggaccgaggc
     6061 ctcgcccgga tgctgcacgt cggtgacgat cggcggtggc gggacgagca gcgagtgcaa
     6121 ggacatctcg cacaccaccg cgacgttcgg cggcccgccc tgcgcgagcc ccgagccgag
     6181 ccccgagccg agcccggaac cgagcccgga accgagcccg gaaccgagcc cggaaccgag
     6241 cccggaaccg agcccggagc cgagccccga gccgagcccg gaaccgagcc cggaaccgag
     6301 cccggaaccg agcccggaac cgagcccgga gccgagtccg gagccgagcc cggagccgag
     6361 cccggaaccg agcccggagc cgtagtccta cttcgacgct gtcgtcgaac gcggccctag
     6421 cgccgttccg gatcggtcct cgacacgacc atcgttcccc gatcgatccg gtagcgcagg
     6481 ccggtcgcgg tcatcgcggc ctcgagcgct tcgtcgactc ggagtagggc gagcgagccc
     6541 cgcaagatcg acgagtccgc cgagcgttcg gtcatcgcgt ccgcgtaggc gatggaccaa
     6601 ccgcgctcgc gcgcgaccca ggcgaggaag gcgctcatcg gctcgccgcc gaagtcgggg
     6661 accggagtga ccccgagcgt ccagtcccac tcgacgccgt gagacggaat cgcgccgtgc
     6721 gtctccgttc cgtccgggcg aacgcggagc tcggcgcctt gaccgacggt ccagttgcgc
     6781 tccgccgaac gcacctcgac ccgcccgtca cgcaggcgca c
//
LOCUS       DASPGN010000563        14353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_808439, whole genome shotgun sequence.
ACCESSION   DASPGN010000563 DASPGN010000000
VERSION     DASPGN010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 14353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14353
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808439"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VD788_16215"
     CDS             complement(<1..854)
                     /locus_tag="VD788_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77856.1"
                     /translation="MPGSRRSEPPHAPTGGERLVGAVTEIDGVVVLPVLHERLEFADC
                     VRRLMREVRFDAVAVEIPSSLERAWLGAVDRLPQVSGLLYENAAGRTIFLAVQPADPL
                     VEAARAAREAGLPVRCADLDVDGYADYRDPLPDPYCVKRLGLAAVCRSFDAVPRRRDP
                     EDGRREAAMAYHARELRAQGARHVLLVCGMHHARSVADELGREQAIPHTTPARRDLRT
                     VHLHPDSLSEVLAEIPFYVAVYERHRAGCPAPRPPHAGGAPAVRRGPFRVISGEGRER
                     ADRVAEAI"
     gene            997..1800
                     /locus_tag="VD788_16220"
     CDS             997..1800
                     /locus_tag="VD788_16220"
                     /inference="COORDINATES: protein motif:HMM:NF018406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HXV77857.1"
                     /translation="MRKFSIYVTPYYSFFSRALYVDVARRWRAGVFVYLLLVLALSWI
                     PFIVRAQSSLSKFVRDDAPAVVEQIPPITIERGRVSAAVEQPHFIQSADTGELFGIID
                     TTGRFTSLDDTSAKVLLARSQLFARKNETETRVYDLSTIQHFYVDGPKISKWLGVLET
                     WGAVMLYLLAVVGSYAYRLVQALFYSIIGLAISTSLRSGLTYGAVLRIVSVAITPAIL
                     VKTAVGLTGLTVPVAWGAYFLVAMAYTTFGISSNRNEPAEERALEPIAG"
     gene            complement(1797..2273)
                     /locus_tag="VD788_16225"
     CDS             complement(1797..2273)
                     /locus_tag="VD788_16225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77858.1"
                     /translation="MLRKGRRLFTVASVLVLVVASAHTLGMLSEPREAADVAIVESMQ
                     AHRFELGLGMSPSVFDIQMSLGLTMTVFLVFVGVGNLVIAGRDPWIAAGVLRFAAVLN
                     ATCMWVLVLLYWFYRIPPPLISFALLGLLFSFAVRTTRDAGPSARSGSPRPDETGG"
     gene            2359..2763
                     /locus_tag="VD788_16230"
     CDS             2359..2763
                     /locus_tag="VD788_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77859.1"
                     /translation="MSNKLVVVAAVLVLGFTAGTAQTEPPVPSVGWIADDLNLPLRYG
                     TAVEFAFQYEVHLVGGEDPEGDATLTLELCENHRCTTSTLAPAEGIRFLRYGLDPSQY
                     HRGRNLYTFTLTLTDNGITSDDTLTIQAVVRP"
     gene            complement(2857..3141)
                     /locus_tag="VD788_16235"
     CDS             complement(2857..3141)
                     /locus_tag="VD788_16235"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXV77860.1"
                     /translation="MGTKLYVGNLPFTADESEVRALFERNSRKVEDVKLITDRETGRP
                     RGFGFVEMGSRDDADKAVRELNGYELGGRRLNVNEAREREGGDGGRRRKF"
     gene            complement(3366..5531)
                     /locus_tag="VD788_16240"
     CDS             complement(3366..5531)
                     /locus_tag="VD788_16240"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HXV77861.1"
                     /translation="MHGTHIIGFTLLLSAWPGFATVTAALNEECRDCHDQRVGHLAAS
                     AHAALACASCHEGAAGHVIDPASVLPRVHFDLELCGACHTDQYGTYTYGDDWKTLFGG
                     SPEGWSKLNDFSHYNDIIDGYGFTREYNEERSHNVMLEDHHDVTRGKKETCLQCKSTK
                     VAYYWDSDAERTIEQDTFVFAGHMTQGLLVPAGTTVAMRTDRAAPRPKNHEVQVLVTL
                     PDGTRYASFDYPGASADGTWLWAALYALTVNELPAGSPTRLSGNGCNHCHDPHRVGRD
                     PATGELVGFRIIRKALLDAIARRGINPYAPASPTVFDGDTPLSLDEGIALCAQCHVEY
                     VCGNSPIDGIDRDYFPWAKASELEQIYSTEFPGWGDHAEMKYIQDWVHGAGALSSTHA
                     PANGVPYLTPAPIGVELIKSQHPEAETYWGSRHYGNAAQCFVCHMPKVTRVTDGTRFT
                     SHWMASPLKYMSPAPVADFAAAFALQVEDGIIPPCAACHSGNLARMKTKAERIQSDTY
                     NDARTVESALVSSLAAIKGARDALAAGQSVDPVLFETAIADHRAAHVRWENLVVSENS
                     MGFHNPNEVAGELSVALVLAQSARQRALDGTNCAAGCGPSSGSVPDGGWVAGVPLTIG
                     RNAAGELELSWGASCVSTDEDFAIYEGTLGQFTSHVPRQCSTGGATSAVVEPSAGSSY
                     YVVLPHNTIWEGSHGYRSTGSERPRGSSNCLPQAVGVCR"
     gene            complement(5712..6692)
                     /locus_tag="VD788_16245"
     CDS             complement(5712..6692)
                     /locus_tag="VD788_16245"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV77862.1"
                     /translation="MSRRRSTLVVAVLLLGASRLPAVELAEGVELLAGSFVAGRQPDG
                     NTVVLTGSDGLIVVDTGRHDEHTRRIVEFARAAGQPVRFVVNTHWHLDHVGGNPSLRR
                     EFPRARVVSSDAIRGAMRGFLADYRRQLEQLVSEAETDEAARPYRSELAILDAGEALA
                     PDDVVTGSGPRELAGRKLEIHLERYAVTAGDLWIHDPASRIVIAGDLVTLPAPFLDTA
                     CPDGWSASLARIADVDFTLLVPGHGPPLDRAGLARYRSAFDGLLACAASEESRQRCVD
                     GWIDGGGELIAASDRELARAVVGYYVEQVLRGDPAQTAARCPPAGGDGLR"
     gene            complement(6719..7915)
                     /locus_tag="VD788_16250"
     CDS             complement(6719..7915)
                     /locus_tag="VD788_16250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HXV77863.1"
                     /translation="MLRRSELRLKKDEDRRLRAGHLWVFANEIDVAATPLDGFEPGAP
                     VLIVSHGGTPLGTGYVNPHSLIAARLCSRDRERPWGPQLIAERLRLALELRERLFPTP
                     YYRLAYGESDGLPGLTVDRFGDVLVVQVTTAGVERFVEQVVAALVKLTAARAVWLRND
                     TAVRDLEGLARASYAGHGELPDTLRVEEGELRFAIPAAAAQKTGWFYDQRDNRAGLTR
                     WASGARVLDLFAYAGAWGLTAAAAGAAEVLCVDASSAAVGAVRGNATANRLPVEVEQA
                     DAFDALGRLHEAGRRFDVVVVDPPALVKRRKDLRHGRDAYHRLNRAALALLEPGGLLV
                     SSSCSHHLDEAMLIDVVRREAIAARRTLQIVAVGGQGADHPVHPAMPETRYLTCLYGR
                     VTAAGY"
     gene            complement(7899..8612)
                     /locus_tag="VD788_16255"
     CDS             complement(7899..8612)
                     /locus_tag="VD788_16255"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HXV77864.1"
                     /translation="MRRSWVSVLAVSVFVSAIVMTGGWAAAKQGWSFNATIIEACSCP
                     MFCQCYFNPQPASHAHHGGEDHFCRFNNAFRVNKGTYNGVKLDGAKFWVAGDLGGDFS
                     NGQMDWAVLHFDSAVTPEQRAGIQAILGHVYPVEWNSFAVGADGAMEWKADKDRAEAR
                     LDGGKVAEVVLNRQQGNTSDPIVIHNLKYWGVPRNDGFVLMPNEVEAYRAGDTAFEFK
                     GTNGFMITLDMNSKDVATK"
     gene            complement(8681..9967)
                     /locus_tag="VD788_16260"
     CDS             complement(8681..9967)
                     /locus_tag="VD788_16260"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV77865.1"
                     /translation="MQATDIDRTTLDSARATVDGVYRADSRRVLATLIRLLGDFDLAE
                     EALHDAFAAAIERWPADGVPANPWAWLVSTGRFKAIDKLRRLERFDRSIPELVRRLEP
                     GAACEDRHDVEGLDDDRLRLVFTCCHPALPPDGQVALTLREVCDLTTEEIAHAFLVAP
                     ATVAQRIVRAKSKIRDARIPYRVPARAELASRLDSVLRVIYLVFNEGYSASSGTVLTR
                     HELSAEAIRLGRLVLELLPDSEVVGLLALMLLHESRRAARTSPAGDLILLEDQDRSRW
                     NRELIAEGSVLVQHAFESRRFGPYTLQAAIAAVHANAPSAADTDWNRIVGYYDLLLRA
                     DPSPVIELNRAAAIALRDGPGAGLTLIDAILARDELTDYHLAHSARAELCRRLGRSSE
                     ARTSYRRALELTRQEPERRFLERRLAELSRSASGGG"
     gene            complement(9974..10336)
                     /locus_tag="VD788_16265"
     CDS             complement(9974..10336)
                     /locus_tag="VD788_16265"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HXV77866.1"
                     /translation="MARYVDGFVVPVPKKKVATYRRLARLAGKVWREHGAVEFVECIA
                     DDVKKGKWTSFPQSVKLKPGEVVFFSWIAFKSRRHRDRVNAKVMKDPRLARMMEPGKM
                     PFDAKRMIYGGFKSLVEL"
     gene            10510..11772
                     /locus_tag="VD788_16270"
     CDS             10510..11772
                     /locus_tag="VD788_16270"
                     /inference="COORDINATES: protein motif:HMM:NF039990.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HXV77867.1"
                     /translation="MGVFRHLGPILSFTTLSIGATLLGTGPSRAAAASAPGRSHKDHA
                     SQPAHQQKPADESDKGLFPDALQIPGTEIFVKFGGYAKVDFIQDLDPIGNESQFKTDT
                     IPVDGTPEADQDGKTTIQARETRFNVDFRTERQGTKLRAFFEGDFYGDGDSFRMRHAY
                     GEAGRLLGGQTWTTFMDISVRPLTIDYEGPDGELFVRQAMIRYTQPLSARWTWAIAVE
                     DPSPQFVVPGTLAGSPRSEVPDVPTYFRYAAERGHVQLAAIARQIRFDGTQGSPDLDE
                     IGWGVSGTFRFKVYEKDELMGQLAHGEGIGRYVESMIGQNVDALITPANELRTIPLTA
                     TVIGYIHHWSDALRSGISYAIADLDDDPLLPTTTIARTEDLRLNLIHSPIPLLDYGVE
                     VLHGRRENQDDASGDAWRVQFAMTVHFN"
     gene            11786..12637
                     /locus_tag="VD788_16275"
     CDS             11786..12637
                     /locus_tag="VD788_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HXV77868.1"
                     /translation="MCSRPTGIRCGILLPICWLVILGCGGPPPEPAEPTESAPAEPAE
                     PPHWDYGTEHGPSAWGELSPDYAACGTGRSQSPIDISASVATGLPALAAQYRPVTLSI
                     VHQEHKADEINTGHTIQVNYPAADTLTVGDEAYALVQYHFHSPSEHAVAGRHFPMEMH
                     LVHRTSDGKLAVVGVFIAEGAANPAFDPVWANLPAEKGDEQQLEHVTVDVDRLLPADR
                     TTYRYDGSLTTPPCSEGVKWFVMTTPIEMSAEQIGRFRAIVDGNNRPVQPLNGRAIAT
                     DAVGDPA"
     gene            12718..13674
                     /locus_tag="VD788_16280"
     CDS             12718..13674
                     /locus_tag="VD788_16280"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXV77869.1"
                     /translation="MERPRVAVVGPGAVGGYFGGMLARAGARVTLVGRPGTALGAHLS
                     QVARHGLRIEGARIRETIAVEVTDTPRAVAGAELVLFCVKTVDTDEAASGIAPYVRDG
                     AIVVSLQNGVDNVERLRRAGVDALAAVVFVGAAVERPGVIDHRGRGDLVVGALAGGSR
                     AGAPTDAGRVAVWFERAGVPCRVAETIERELWIKLIINSMSNPIAAVTGATYAEIAEF
                     EPAWRLAVVIAGEAVGVGNAAGVGLELDEVLARARAVIESVGASTASTRQDVERGRAT
                     EIDALNGYLSRRGSELGIPTPVNDAMHALVKLRERRGGAQQP"
     gene            complement(13679..>14353)
                     /locus_tag="VD788_16285"
     CDS             complement(13679..>14353)
                     /locus_tag="VD788_16285"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV77870.1"
                     /translation="RPESSRSGSRPESLFESVGAGLGYLRRRTGHTTLLAIGALVNLC
                     MVPAFSLLPLLVVEQGGGAARLGGMTSIFGAGTLAGGVLLGLWGGFHRRIHTTALGLL
                     GAGLATVALALAPSAGLALAAVAAVGLAIPLVNGPIQALLQATIAADYQGRVFTLYGS
                     VCAMMTPIGLAAAAPIADWLGVRAWFVAGGTICLGMALLGALSPSLRRIEEADAVGAA
                     VLRQQS"
BASE COUNT         2256 a         5004 c         4892 g         2201 t
ORIGIN      
        1 tcgatcgcct cggcgacgcg atccgcgcgc tcccgccctt cgccggagat gacgcgaaac
       61 gggccccgac gcacggcggg cgcgccaccg gcgtgcggtg gacgcggcgc cgggcagccg
      121 gcgcgatgtc gctcgtagac ggcgacgtag aacgggattt cggccaacac ctcggagagc
      181 gagtcggggt gcaggtgaac cgtacgcagg tcgcgccggg cgggagtggt gtgcgggatc
      241 gcctgttcgc ggcccagctc gtcggcgacg ctccttgcgt ggtgcatgcc gcagaccagc
      301 aggacgtgac gggcgccctg cgctcgcaac tcgcgagcgt ggtacgccat cgccgcctcg
      361 cgccgcccgt cctcggggtc acgccgccgc ggaaccgcgt cgaacgagcg gcaaaccgcc
      421 gccagcccca gacgcttgac gcagtaggga tcgggcagcg ggtcgcgata gtcggcatag
      481 ccgtcgacgt cgaggtcggc gcaacgaacc ggtagcccgg cctctcgggc ggcgcgcgcc
      541 gcctcgacca gcgggtcggc gggttggacg gcgaggaaga tcgtccgtcc cgccgcgttc
      601 tcgtagagca gaccggagac ctgcggcagc cggtcgaccg cgccgagcca tgcgcgttcg
      661 agactcgacg ggatctcgac cgccaccgcg tcgaaacgga cctcgcgcat cagccggcga
      721 acgcagtcgg caaactcgag acgttcgtgc agcaccggca ggacgacgac cccgtcgatc
      781 tcggtcaccg caccaaccag ccgctcgccg cccgtcgggg cgtgcggcgg ctcgctccgt
      841 cgcgaaccag gcatgccggg actatacacg ccgtcgctcg tcacggaaaa acccccggct
      901 ttcggtccga gcggcccgcg ccgccaccgg gaactataca taagccaagc gagtcccgac
      961 cgacgaccgg ccacggctct cgtaagtgag gacgtcatga ggaaattctc gatctacgtc
     1021 acgccgtact actccttctt ctcccgcgcg ctgtacgtcg acgtcgcccg ccggtggaga
     1081 gccggcgtgt tcgtctacct gctgctcgtg ttggcgctgt cgtggatacc gttcatcgtc
     1141 cgggcccaat cgtcgttgtc caagttcgtc cgcgacgacg ctccggccgt cgtcgagcag
     1201 atcccgccga tcacgatcga gcgggggcgg gtctcggccg cggtcgagca accgcatttc
     1261 atccagtcgg cggatacggg cgagttgttc gggatcatcg acacgaccgg ccgattcacc
     1321 tcgctcgacg acacgtcggc caaggtgttg ctcgctcgat cccagctgtt cgcgcgaaag
     1381 aacgagaccg agaccagggt ctacgatctt tcgaccatcc agcacttcta cgtcgacgga
     1441 ccgaagatca gcaagtggct cggcgtactc gagacgtggg gcgcggtgat gctgtatctg
     1501 ttggcggtcg tcggctccta cgcctaccgg ctcgttcagg cgctgttcta ctcgatcatc
     1561 ggcctggcga tctcgacgag tctccgctcc gggctgacct acggcgccgt gttgcggatc
     1621 gtgtcggtgg cgatcacgcc ggcgatcctg gtcaagaccg ccgtcggtct gacgggtctg
     1681 acggtgcccg tggcctgggg cgcctacttc ctcgtcgcga tggcctacac gaccttcgga
     1741 atctcgtcga accgaaacga gcccgcggaa gagcgagcgt tggagccgat cgcgggctag
     1801 ccgcccgtct cgtcagggcg cgggctgccg ctgcgcgccg acggaccggc gtcacgcgtg
     1861 gtgcggacgg cgaagctgaa cagcaggccc aacagcgcga agctgatcag cgggggcggg
     1921 atgcggtaga accagtacag cagcaccagc acccacatac acgtcgcgtt cagcactgcc
     1981 gcgaaccgca gcacgccggc ggcgatccac gggtcacggc cggcgatcac gagattgccg
     2041 acgccgacga agacgaggaa gaccgtcatc gtcaggccga ggctcatctg gatgtcgaag
     2101 acgctcgggc tcatgccgag ccccagctcg aaccgatggg cctgcatcga ctcgacgatc
     2161 gcgacgtcgg ccgcctctcg cggctcggac agcataccga gggtgtgcgc cgacgcgacg
     2221 acgaggacca gcaccgaggc gacggtgaac aagcgtctac ccttccggag catgcgccga
     2281 tcctatcacc ggtcgagcgc tcccggccga aagcggatag aatcgggagc ttcagcaggt
     2341 atgctggtgg agttccctat gagcaacaag ctcgtggtcg tggcggcggt cctggtgctc
     2401 gggtttacgg cgggtacggc ccagaccgaa ccaccggtgc cgtcggtggg ctggatcgcc
     2461 gacgatctca acctgccgct gcgctacggt acggccgtcg agttcgcttt ccagtacgag
     2521 gtccatctcg tcggcggtga agacccggag ggggacgcca cgctgacgct cgagctgtgc
     2581 gagaaccacc gctgtacgac ctcgacgctc gcgcccgccg aggggatccg gttcctgcgc
     2641 tacgggctcg acccgtcaca gtatcaccgc ggccggaacc tctacacctt cacgctgacg
     2701 ttgaccgaca acggcatcac ttcggacgac acgttgacga tccaggccgt cgtccggccg
     2761 taggtcgaac gcggcgaccg cgccgtcgat tcaccgacga aaaagagggt gcccgacggg
     2821 caccctcttt cgaccgcttc gtcgatggcg ttcgtgctag aacttacgcc gccgtccgcc
     2881 gtcgccgccc tcacgctcgc gcgcctcgtt gacgttcagg cgccgcccgc cgagctcgta
     2941 gccgttcagt tcgcggacgg ccttgtcggc atcgtcgcgg ctgcccatct cgacgaaccc
     3001 gaaacctcgg ggacgcccgg tctcgcggtc ggtgatcagc ttgacgtcct ccaccttgcg
     3061 cgagttccgc tcgaacagcg ctcgcacctc ggattcgtcc gcggtgaagg gcaagttgcc
     3121 gacgtacagt ttcgttccca tctcccaaac tcctcgctct ctcgcaagag ggcgtactga
     3181 tcaggttccc ggtccatgac tccgacaacg ggtcaggttc gatgccgaag cgcgacgcac
     3241 ttcggactcg gtttcggcac ggcaaaaaat cgtttctgta cgaattgttg cgaccaggaa
     3301 cctgcggcca cgatacaggt cccgggccgc gctgtcaaac tccgccggcc gctctcggcc
     3361 caccttcagc ggcagacccc gaccgcctgc ggcaagcagt tcgagctgcc tcgcggtcgc
     3421 tcgctgccgg tgctgcggta gccgtgcgat ccctcccaaa tcgtgttgtg cggcaacacc
     3481 acgtagtacg agctgccggc ggacggctcg accaccgcgc tcgtcgcgcc tccggtgctg
     3541 cactggcgcg gcacgtggct ggtgaactgt ccgagcgtcc cctcgtagat cgcgaagtcc
     3601 tcgtccgtcg agacgcagga tgccccccag ctcagctcga gctctcccgc cgcgttccgg
     3661 ccgatcgtca gcggaacgcc cgcgacccaa ccgccatccg gcacgctacc cgaggacggg
     3721 ccgcagcccg cggcgcagtt cgtgccgtcg agcgcgcgct ggcgcgccga ctgcgcgaga
     3781 accagcgcga cggacagttc gcccgccacc tcgttcgggt tgtggaagcc catgctgttc
     3841 tccgagacga cgaggttctc ccagcgcacg tgggccgcgc ggtggtccgc gatcgcggtc
     3901 tcgaacagca ccggatcgac ggactgcccg gccgccagcg cgtctcgcgc acccttgatc
     3961 gcggccagac tgctcaccag cgcggactcc accgtccggg catcgttgta ggtgtcactc
     4021 tggatccgct ccgccttggt cttcatccgg gcgaggtttc cgctgtgaca ggcagcgcac
     4081 ggcgggatga tcccgtcttc gacctgcagt gcgaaggccg cggcgaagtc cgccaccggc
     4141 gcgggcgaca tgtacttgag cggcgaggcc atccagtggc tggtgaaccg ggtgccgtcg
     4201 gtgacccggg tgaccttggg catgtgacag acgaagcact gggcggcgtt gccgtagtgg
     4261 cggctgcccc aataggtctc ggcctccggg tgctggctct tgatcagctc gactccgatc
     4321 ggggcgggcg tcaggtacgg caccccgttg gccggagcgt gggtgctcga cagcgcgccg
     4381 gcgccgtgca cccagtcctg gatgtacttc atctccgcgt ggtcgcccca tcccgggaac
     4441 tcggtggagt agatctgctc cagctccgac gccttggccc acgggaagta atcgcgatcg
     4501 atcccgtcga tcgggctgtt gccgcagacg tactcgacgt ggcactgggc gcacagcgcg
     4561 attccctcgt ccaggctcaa cggggtgtcg ccgtcgaaga cggtcggact cgccggtgcg
     4621 taagggttga ttccccggcg cgcgatcgcg tcgagcagcg ccttgcggat gatgcggaag
     4681 ccgacgagct cgcccgtcgc cggatcgcgg cccacccggt gcgggtcgtg gcagtgattg
     4741 cagccgttcc ccgacaaccg cgtcggcgac cccgccggca gctcgttgac ggtcagggcg
     4801 tacagggcgg cccacagcca cgtgccgtcg gcggaggcgc ccgggtaatc gaagctggcg
     4861 tagcgcgtgc cgtcgggcag cgtcaccagc acctgcacct cgtggttctt cggccgcggt
     4921 gccgcgcgat cggtgcgcat cgcgaccgtc gtgcccgccg gcacgagcag tccctgcgtc
     4981 atgtgaccgg cgaacacgaa cgtgtcttgc tcgatcgtcc gctcggcgtc cgagtcccag
     5041 taataggcca ccttggtcga cttgcactgc aggcaagtct ctttcttgcc ccgcgtgacg
     5101 tcgtgatggt cctcgagcat gacgttgtgc gagcgctcct cgttgtattc gcgggtgaag
     5161 ccgtatccgt cgatgatgtc gttgtagtgc gagaagtcgt tgagcttgct ccacccttcg
     5221 ggagagccgc cgaacaacgt cttccagtcg tcgccgtagg tgtaggtccc gtactggtcg
     5281 gtgtgacacg ccccgcacag ctcgagatcg aagtgcacgc ggggcaagac cgacgccgga
     5341 tcgatgacgt ggcccgccgc cccctcgtga cagctcgcgc aggccagggc ggcgtgcgcg
     5401 gaggcagcga gatggccgac ccgctgatcg tggcaatcgc ggcactcctc gttgagcgcc
     5461 gctgtgaccg tcgcgaatcc cggccacgcc gacagtagca gggtgaatcc gatgatgtgc
     5521 gtgccgtgca tggtggtgac ccttcggcgt ggaggccgtc gggttcgtcg cgtcgtgcct
     5581 cgatccgcgc ggcctcgatc acccgaatga tagcgggggg tggcggcggg acccatggac
     5641 cattcggtac cgaaccggtc gatttggtgc tcggtcgagc cccgttcgct cccgtctcgt
     5701 tcgcgccgcg ctcagcgcag tccgtcgccg ccggccggcg ggcagcgtgc ggcggtctgg
     5761 gccggatcgc cccggaggac ctgctcgacg tagtagccga cgacggcgcg tgccagctcc
     5821 cggtcgctcg cggcgatcag ctcgccgccg ccgtcgatcc aaccgtcgac gcaccgttgg
     5881 cggctctcct cggaggccgc gcaggccagc agcccatcga aggcgctccg gtagcgtgcg
     5941 aggccggccc gatcgagcgg cggcccgtgg cccggcacga gcagcgtgaa gtcgacgtcg
     6001 gcgatgcgcg cgagcgacgc gctccagccg tcggggcagg ccgtgtcgag gaacggcgcc
     6061 ggcaacgtca ccaggtcacc ggcgatcacg atccggctcg ccggatcgtg gatccacaga
     6121 tcacccgcgg tgaccgcgta tcgttcgagg tggatttcga gcttccgacc ggccagctcc
     6181 cgtggtccgg agccggtcac gacgtcgtcg ggcgcaagcg cctcgccggc gtcgaggatc
     6241 gccagctccg agcggtacgg ccgcgccgcc tcgtccgtct ccgcctcgga tacgagctgt
     6301 tcgagctgcc ggcggtagtc ggcgagaaaa ccgcgcatcg caccgcggat cgcgtcgctc
     6361 gacaccacgc gcgcacgcgg aaactcgcgg cgcaacgacg gattgccgcc gacgtggtcg
     6421 aggtgccagt gcgtgttgac gacgaaacgc acgggctgcc cggcggcgcg cgcgaactcg
     6481 acgatccgcc gggtgtgctc gtcgtggcgg ccggtgtcga cgacgatcag gccgtcgctc
     6541 cccgtcagca cgaccgtgtt gccgtcgggc tgccggccgg cgacgaagct cccggcaagc
     6601 agctcgacgc cctcggcgag ttcgaccgcc ggtagtcgcg aggcgccgag cagcaggacg
     6661 gcgacgacca gtgtcgaacg gcgtcgggac atcgtcgttc tcccgcgaag gtcggcggtc
     6721 aatagcccgc cgccgtgacc cgcccgtaga ggcaggtgag gtagcgggtc tcgggcatcg
     6781 ccggatggac cggatgatcg gctccttgtc cgccgacggc gacgatctgc agcgtccgcc
     6841 gggccgcgat cgcctcccgc cgcacgacgt cgatcagcat cgcctcgtcg agatggtggg
     6901 agcaggagct gctgacgagc aggccgccgg gctcgagcag cgcgagggcc gcacggttca
     6961 gccgatggta ggcgtcgcgc ccgtggcgca agtccttgcg gcgcttgacc agcgccggcg
     7021 gatccaccac gaccacgtcg aagcggcggc ccgcctcgtg caggcgaccg agcgcgtcga
     7081 acgcgtcggc ctgctcgacc tcgacgggca gacggttggc ggtcgcgttg ccgcgaacgg
     7141 cgccgaccgc ggccgacgag gcgtcgacgc acagcacctc ggccgcaccg gccgccgccg
     7201 ccgtcaggcc ccacgcgccg gcgtaggcga acaggtcgag cacgcgtgcc ccgctcgccc
     7261 aacgtgtcag gccggcccgg ttgtcgcgct ggtcgtagaa ccagcccgtc ttctgcgccg
     7321 cggccgccgg gatcgcgaac cggagttcgc cttcctcgac tcgcagcgtg tcgggcagct
     7381 cgccgtgccc ggcataggac gcacgcgcga gtccctcgag gtcgcgcacc gccgtgtcgt
     7441 tgcgcagcca caccgcgcgg gctgcggtca gcttcaccag cgccgcgacc acctgttcga
     7501 cgaaccgctc gacgcccgcg gtcgtgactt gaaccaccag cacgtcgccg aagcgatcga
     7561 cggtcaagcc cggaaggccg tcggactcgc cgtaggccag ccgatagtac ggcgtcggga
     7621 acaaccgctc ccgcagctcg agcgcgagcc gcagccgctc cgcgatcaac tgcggacccc
     7681 acggccgctc gcgatcccgc gagcacagcc gggcggcgat cagcgagtgc gggttgacgt
     7741 agcccgtgcc gagcggggtg ccgccgtgcg agacgatgag gacgggcgcc ccgggctcga
     7801 aaccgtcgag cggcgtcgcc gcgacgtcga tctcgttggc gaagacccac aggtgtcctg
     7861 cccggaggcg tcgatcctcg tccttcttga gcctgagctc acttcgtcgc aacatccttc
     7921 gagttcatgt ccagcgtgat catgaagccg ttggtgccct tgaactcgaa cgccgtgtcg
     7981 ccggcgcggt aggcctcgac ctcgttgggc atcagcacga agccgtcgtt gcgtgggacg
     8041 ccccagtact tgaggttgtg aatcacgatc gggtcgctgg tgttgccctg ctggcgattc
     8101 aacaccacct cggcgacctt gcccccgtcc agccgggcct cggcacggtc cttgtcggcc
     8161 ttccactcca tcgctccgtc cgccccgacc gcgaacgagt tccactcgac cgggtagacg
     8221 tggcccagga tcgcctggat ccccgcgcgc tgttccgggg tcaccgccga atcgaagtgc
     8281 agcacggccc agtccatctg cccgttggag aagtcgccac ccaggtcgcc ggcgacccag
     8341 aacttcgccc cgtcgagctt gaccccgttg taggtgccct tgttcacccg gaacgcgttg
     8401 ttgaagcgac agaagtggtc ctctccgccg tgatgcgcgt gcgaggcggg ctggggattg
     8461 aagtagcatt gacagaacat cggacagctg caggcctcga tgatcgtcgc gttgaacgac
     8521 cagccctgct tggcggcggc ccagccgccc gtcatcacga tcgccgatac gaaaaccgag
     8581 acagccagca cactgaccca ggacctacgc atcgtcccct ccttccgctc gaatgaccga
     8641 gacggtcgtc gattgtaacc cgggagggtt cgatcgggaa tcagccaccg ccgctggccg
     8701 agcgagacag ctcggcgagt cgccgctcga ggaaccgtcg ctccggctcc tgccgggtca
     8761 gctcgagcgc cctgcgatag gaggtgcgcg cttcggacga tcgtccgagc ctgcgacaga
     8821 gctcggctcg cgccgagtgg gcgaggtggt agtcggtcag ctcgtctcga gccaggatcg
     8881 cgtcgatcag ggtcaggccg gcgccaggtc cgtcccgcag cgcgatcgcc gcggcgcggt
     8941 tcagctcgat caccggcgac gggtcggcac ggagcagcag gtcgtagtaa ccgacgatcc
     9001 gattccagtc ggtgtcggct gcggacggtg cgttggcgtg tacggccgcg atcgcggcct
     9061 gcagcgtgta cgggccgaac cgcctcgact cgaacgcgtg ctggacgagc accgagccct
     9121 ccgcgatcag ctcgcggttc caacgcgagc ggtcctggtc ctcgagcagg atcagatcgc
     9181 cggctggcga ggtgcgcgcc gcgcggcgtg actcgtggag cagcatcagc gccagcagcc
     9241 cgaccacttc tgagtccggc agcagctcga gcacgagccg tccgagacgg atcgcctcgg
     9301 ccgacagctc gtgtcgtgtc aggacggtgc ccgaactggc ggagtagccc tcgttgaaca
     9361 cgaggtagat cacgcgcagc accgagtcga gacgcgacgc gagctcggct cgcgctggaa
     9421 cccgataggg gatgcgcgcg tcgcggatct tgctcttcgc gcgcacgatg cgctgggcga
     9481 ccgtcgcggg ggcgacgaga aacgcgtgcg cgatctcctc cgtcgtcagg tcgcaaacct
     9541 cgcgcagcgt cagcgcgacc tgcccgtcgg gtggcagcgc cgggtggcag caggtgaaga
     9601 ccagtcgcag ccggtcgtcg tcgagcccct cgacgtcgtg gcggtcctcg cacgccgcgc
     9661 ccggctcgag ccggcggacg agctcgggga tcgaccggtc gaatcgttcg agtcgccgga
     9721 gcttgtcgat cgccttgaag cggccggtcg agacgagcca agcccacggg ttggccggta
     9781 ccccgtcggc cggccaccgt tcgatcgccg cggcgaacgc gtcgtgcagc gcctcctcgg
     9841 cgaggtcgaa atcgccgagc agacggatca gcgtcgccag cacgcggcgc gagtcggcac
     9901 ggtagacccc gtcgaccgtc gcgcgggcgg agtcgagcgt cgttcggtcg atgtccgtcg
     9961 cctgcactcc cccctataac tcgaccagcg acttgaagcc gccgtagatc atccgcttcg
    10021 cgtcgaacgg catctttccc ggctccatca tccgggccag gcgcgggtcc ttcatcacct
    10081 tggcgttgac gcggtcgcga tgccggcggg acttgaacgc gatccacgag aagaacacca
    10141 cctcgcccgg cttgagcttg acgctctgcg gaaaggaagt ccacttgccc ttcttgacgt
    10201 cgtcggcgat gcactcgacg aactcgaccg cgccgtgctc gcgccacacc ttgccggcga
    10261 gccgcgccag acggcgatag gtcgcgacct tcttcttcgg tacgggcacg acgaacccgt
    10321 ccacgtaacg cgccatgtca aacctcctgt tccgtcatct ccaggcgcac ctccgagggt
    10381 tgccggacac gggattgtcg cccggcggtc gctgttttcg acagcctccg cggcggaatt
    10441 tttcatcgac atcggctcac cgcgtgtcct atcctcgcgg cggccgatct cgtacgagcg
    10501 aggtgaggaa tgggggtttt ccggcatctc ggcccgattc tatcgttcac caccctgtcg
    10561 atcggggcca cgctcctcgg gaccgggccg agccgggccg ccgcggcgtc cgcgcccggg
    10621 cgctcgcaca aggaccacgc gagtcagccg gcccatcagc agaagcccgc ggacgagtcg
    10681 gacaagggct tgttcccgga cgccctacag atcccgggga ccgagatctt cgtgaagttc
    10741 ggtggttacg ccaaggtcga cttcattcaa gacctcgacc cgatcggcaa cgagtcccag
    10801 ttcaagaccg acacgatccc ggtcgacggg acgccggagg ccgaccagga cggcaagacg
    10861 acgatccagg ctcgcgagac acgcttcaac gtcgactttc gcaccgaacg gcaggggacg
    10921 aagctgcgtg ccttcttcga gggcgatttc tacggcgacg gtgactcgtt ccgcatgcgc
    10981 cacgcctacg gcgaggccgg ccgtctgctc ggtggacaga cctggacgac gttcatggac
    11041 atctcggtgc gaccgctgac gatcgactac gagggaccgg acggcgagct gttcgtacgg
    11101 caggcgatga tccgttacac gcagccgctg tccgcgcgct ggacctgggc gatcgcggtc
    11161 gaagacccca gcccgcagtt cgtcgtaccc ggaacgctcg cgggcagccc gcgcagcgag
    11221 gttcccgacg tcccgaccta cttccggtac gccgccgagc ggggccacgt gcagctcgcc
    11281 gccatcgcgc gtcagatccg cttcgacggc acgcagggct cgcccgatct ggacgagatc
    11341 ggctggggtg tcagcggcac gttccgcttc aaggtgtacg agaaggacga gctgatgggt
    11401 cagctggccc atggtgaggg gatcgggcgt tacgtcgagt cgatgatcgg gcagaacgtc
    11461 gacgcgctga tcacgccggc gaacgagctg cggacgatcc cgttgacagc gaccgtgatc
    11521 ggctacatcc accactggag cgacgcgctg cgctccggga tttcctatgc gatcgccgat
    11581 ctcgacgacg acccgttgct gcccaccacg acgatcgcca gaaccgagga cctgcggctc
    11641 aacctgatcc attcgccgat tccgctgctc gactacggcg tcgaggtgct gcacggccgc
    11701 cgtgagaatc aagacgatgc atcgggcgac gcgtggcgcg tccagttcgc catgaccgtc
    11761 cacttcaact gagggagtca cgagtatgtg tagtcggccg accggaatcc gctgtggcat
    11821 tctgctgccg atctgctggt tggtcattct gggctgcggt ggcccgccgc cggaaccggc
    11881 ggagccgacc gagtcggcgc cggccgagcc cgccgagccc ccgcactggg actacggcac
    11941 cgagcacggc ccgagcgctt ggggtgagct cagtccggac tacgccgcct gcggcacggg
    12001 tcgcagccaa tcgccgatcg acatctcggc gagtgtcgcg accggacttc cggcgctcgc
    12061 cgcgcagtac cgcccggtca cgctgtcgat cgtgcaccag gagcacaagg ccgacgaaat
    12121 caacaccggg catacgatcc aggtcaacta tccggccgcc gatacgctga cggtcggcga
    12181 cgaagcctac gcgctcgtcc agtaccactt tcacagcccg agcgagcacg cggtcgcggg
    12241 acggcacttc ccgatggaaa tgcacctcgt gcaccgaacc tcggacggca agctggcggt
    12301 cgtcggcgtg ttcatcgccg agggcgcggc gaacccggcg ttcgatccgg tctgggccaa
    12361 cctgcccgcc gagaaaggcg acgagcagca gctcgagcac gttacggtcg acgtcgaccg
    12421 actcctgccg gcagaccgta cgacctatcg ctacgacggc tcgctcacga ctccgccgtg
    12481 ctcggaaggc gtcaagtggt tcgtcatgac gacgccgatc gaaatgtcgg cggagcagat
    12541 cgggcgcttc cgtgcgatcg tcgacggcaa caaccggccg gtccagccgc tcaacgggcg
    12601 cgccatcgcg acggacgcgg tgggggatcc ggcgtgaccg gtcacgcggt cacgcggggg
    12661 tgcccagcgc ccggttcccg cgacgaacct gcggtcgccc gacgcgtata cagcgagatg
    12721 gagcggccgc gcgtagcagt cgtcggtccc ggcgccgtcg gtggctactt cggcgggatg
    12781 ctggcccgcg ccggagcgcg ggtgacactc gtcgggcgcc cgggaaccgc actcggcgca
    12841 cacctctcgc aggtcgcgcg gcacgggttg cgcatcgagg gcgcgagaat ccgggagacg
    12901 atcgcggtcg aggttaccga cacgccgcgc gcggtcgccg gtgccgagct cgtgctgttc
    12961 tgcgtcaaga ccgtcgacac cgacgaggcc gcgagcggga tcgcgcccta cgtgcgggac
    13021 ggcgcgatcg tggtcagcct gcagaacggc gtcgacaacg tcgagcgact ccgccgagcg
    13081 ggcgtcgatg cgctcgctgc ggtcgtcttc gtcggcgcgg ccgtcgagcg ccccggcgtg
    13141 atcgaccatc gcgggcgggg tgatctggta gtcggcgcgc tggccggcgg cagccgggcc
    13201 ggagccccga ccgacgccgg ccgtgtggcc gtctggttcg agcgcgccgg cgttccctgc
    13261 cgcgtggccg agacgatcga gcgcgagctg tggatcaagc tgatcatcaa ctcgatgtcg
    13321 aacccgatcg ccgcggtgac cggggcgacc tatgccgaga tcgcggagtt cgaacccgct
    13381 tggcgcctgg cggtcgtcat cgccggtgag gccgtcggcg tcggaaacgc ggcgggagtc
    13441 gggctcgagt tggacgaggt gttggcccgg gcacgagcgg tgatcgagtc ggtcggagcg
    13501 tcgacggcgt cgacgcgtca ggacgtcgaa cgcgggcggg cgaccgagat cgacgcgctg
    13561 aacggctacc tgtcgcggcg aggtagcgag ctcggtattc cgaccccggt caacgacgcg
    13621 atgcacgcgc tggtcaagct gcgcgaacga cgcggcggcg cgcagcagcc gtgagcggtc
    13681 agctctgctg tcggagcacc gcggcaccga ctgcgtccgc ctcttcgatc cgtcgcagcg
    13741 aggggctgag cgcgccgagc agcgccatgc cgagacagat cgtcccgccg gcgacgaacc
    13801 aggcgcggac gccgagccag tcggcgatcg gcgctgccgc agccagcccg atcggcgtca
    13861 tcatcgcgca cacgctcccg tagagcgtga agacacgtcc ctgatagtcc gcggcgatcg
    13921 tcgcctgtag cagcgcttgg atcgggccgt tcacgagcgg gatcgccaag cccacggccg
    13981 cgaccgcggc cagcgccaaa ccggccgacg gcgcgagcgc gagcgccacg gtcgccagac
    14041 cggcgccgag caggcccagc gccgtggtgt ggatccggcg gtggaatccg ccccacagcc
    14101 cgagcaagac cccgccagcg agcgtgccgg cgccgaagat cgacgtcatt ccgccgagtc
    14161 gggcggcacc gcccccctgc tcgacgacga gcagcggcaa caaggagaag gccggcacca
    14221 tgcacaggtt gacgagtgcc ccgatcgcga gcagcgtggt gtggccggtc cgacggcgga
    14281 ggtaaccgag accggcaccg accgactcga acaacgactc ggggcgcgag ccggatcgcg
    14341 agctctccgg ccg
//
LOCUS       DASPGN010000564         2786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1746230, whole genome shotgun sequence.
ACCESSION   DASPGN010000564 DASPGN010000000
VERSION     DASPGN010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1746230"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2786
                     /locus_tag="VD788_16290"
     CDS             <1..>2786
                     /locus_tag="VD788_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXV77871.1"
                     /translation="NIAVDAGLGVDAVDIDAAGAFGEFELGVDLGADANEVAVEKLTL
                     AYEHIDLNVHVDAVHQYAYAPGASREGAAVYEADLRSDGELVQAVYGASTAGGFASDQ
                     LSIALGDGRAGTVGNLVGINVSDQSATVSHNETITIHGNRTEAQQINQITAFIDGSLN
                     SSMESSLAIDLGGDEEVFAEHARAVGGGLTGRLEVLTGAGDDSLLVDLSGNDAPTRAG
                     FTEVSGLSTEITPVEYRLGAMPGDNVSLTVDMAGIDAFELITGDAADEIDLELAYPAV
                     PVPTLRFATGGGNDVIGGTLVGVPGTLDLSLDTGAGDDDVQLEFTEAASIVTGAGAGH
                     PSEVKAFGGATSVEGHSLTAYPGFTGGVRVATGDVNGDGVVDLVTGAGEGAGSHVKVF
                     DGRTGAELRSFFAYPGFTGGVYVAAGDVDGDGYADIITGAGAGGGPHVKVFSGATGAT
                     LQSFFAYTPSFTGGVRVAAGDVNGDGFADIVTGAGPGGGSHVKVFDGLTSAEIRSFFA
                     YPGFTGGVYVAAGDVNGDGLDDIVAGAGAGGPSHVKVFDGSSGGALHSFFAYSPSFTG
                     GVRVAAGDVNGDGFADIVTGAGPGAGPHVKVFDGVTSAAIRSFFAYDPGFLGGVYVAA
                     ADVNPDASDAPAALNLAVNTAGGNDLVALEMASGASRSDALDIDLGTGSDRFALDWQG
                     ALQDPAVDVELDLQVAFGGAEAAALEGDECLVFFLGGVPDRPVVIGMVWNSQDNPPPD
                     DTAPQDDKHLSLALSSTDGFLSLASALTGGAGSDDVRLFFRGTLEVARDAQAPQLSAV
                     IELGDGNDSALVDFSALETGKGTKLAPVGVDLRGGAGVDALAITGTQGADAFTVTGRR
                     VVLEGAGEVNYVGFEVLEVRTLGGDDRVTMTGIDPGTRTLIDGGEGRDTFFGRFDGDF
                     DGELTLLEFEKLTF"
BASE COUNT          420 a          969 c         1000 g          397 t
ORIGIN      
        1 caacatcgcc gtcgacgccg gcctcggcgt cgacgcggtc gacatcgacg cggcgggcgc
       61 cttcggcgag ttcgagctcg gcgtcgatct cggcgccgac gcgaacgagg tcgcggtcga
      121 aaagctcacg ctggcctacg agcacatcga tctcaacgtc cacgtcgacg cggtgcatca
      181 gtacgcgtac gcgcccggcg cgagccgcga aggcgccgcg gtgtacgagg cggacctgcg
      241 ctccgacggc gagctggtgc aggcggtcta cggcgccagc acggccggcg gcttcgccag
      301 cgatcagctg agcatcgcgc tcggcgacgg ccgggccggt acggtcggca atctcgtcgg
      361 catcaacgta tccgatcaga gcgcgacggt ctctcacaac gaaacgatca ccatccatgg
      421 caaccgcacc gaagcgcagc agatcaacca gatcaccgcc ttcatcgacg gctcgttgaa
      481 ctcgagcatg gagagcagcc tcgccatcga cctgggcggc gatgaagagg tgttcgcgga
      541 gcatgcgcgt gccgtgggcg gcggcctgac cgggcgcctc gaggtgctga ccggcgccgg
      601 cgacgacagc ttgctggtcg atctgagcgg caatgacgcg ccgacgcgag ccggcttcac
      661 ggaggtcagc ggactttcaa ccgagatcac gcctgtcgaa tatcgcctgg gcgcaatgcc
      721 cggcgacaac gtctcgctga ccgtagacat ggccggcatc gacgcgttcg agctgatcac
      781 cggcgatgcg gccgacgaaa tcgatctcga gctcgcctac ccggcggtgc cggtgccgac
      841 gctgcgcttc gccaccggcg gtggtaacga cgtgatcggc ggcacgctcg tcggcgtgcc
      901 cggcacgctc gatctgagcc tcgacaccgg cgcgggcgac gacgacgtcc agctcgagtt
      961 caccgaggcg gcgtcgatcg tcaccggcgc gggcgcgggc catccctccg aggtgaaggc
     1021 gttcggcggg gcaacctcgg tcgagggcca cagcctgacc gcctatccgg gattcacggg
     1081 cggcgtgcgc gtcgcgacgg gcgacgtcaa cggcgatggc gtcgtcgatc tcgtcaccgg
     1141 cgccggcgaa ggcgcgggat cgcacgtcaa ggtgttcgac ggccgcacgg gcgccgagct
     1201 gcgcagcttc ttcgcctatc cggggttcac cggcggcgtg tacgtcgccg cgggcgacgt
     1261 cgacggcgac ggctacgccg acatcatcac cggcgccggc gcgggcggcg gcccgcacgt
     1321 caaggtcttc agcggcgcga cgggcgcaac gctgcagagc ttcttcgcct acaccccgtc
     1381 gttcaccggc ggggtgcgcg tcgccgcggg cgacgtaaac ggcgacggct tcgccgacat
     1441 cgtcaccggc gcaggacccg gcggcggatc gcacgtcaag gtcttcgacg gattgacctc
     1501 cgccgagatt cgcagcttct ttgcctatcc gggattcacc ggcggcgtgt acgtcgccgc
     1561 gggcgacgtc aacggcgacg gcctcgacga catcgtcgcc ggcgccggcg ccggcggccc
     1621 gtcgcacgtc aaggttttcg acggctcgag cggcggcgcg ctgcacagct tcttcgcgta
     1681 ttcgccgagc ttcaccggcg gcgtgcgcgt agccgcgggc gacgtaaacg gcgacggctt
     1741 cgccgacatc gtcaccggcg ccgggccggg cgcaggcccg cacgtcaagg tcttcgacgg
     1801 cgtgacctcg gccgcgatcc gcagcttctt cgcctacgac ccgggcttcc tcggcggcgt
     1861 ctacgtcgcc gcggccgacg tcaacccgga cgcgtccgac gcgcctgcgg cgctcaatct
     1921 cgccgtcaac accgcgggcg gaaacgacct ggtggcgctc gagatggcga gcggcgccag
     1981 ccgcagcgac gcgctcgaca tcgatctcgg caccggttcg gatcgcttcg cgctcgactg
     2041 gcagggggcg ctgcaggatc ccgcggtcga cgtagagctc gacctgcaag tcgccttcgg
     2101 cggtgccgag gcggccgcgc tcgaaggaga cgagtgcctg gtgttcttcc tggggggcgt
     2161 tcccgaccgg ccggtcgtca tcggcatggt gtggaactcg caggacaacc cgccgccgga
     2221 cgacaccgcg ccgcaggacg acaagcacct gagcctcgcg ctgtcgagca cggacggctt
     2281 cctctcgctc gcaagcgcgc tgaccggcgg cgcgggcagc gacgacgtgc ggctcttctt
     2341 ccgcggcacg ctcgaggtcg cgcgcgatgc acaggcgccg cagctgagcg ccgtgatcga
     2401 gctcggcgac ggcaacgaca gcgcgctggt cgacttcagc gcgctcgaga cgggcaaagg
     2461 gacgaagctc gccccggtcg gcgtggacct gcgcggcggc gcgggcgtgg atgcgctcgc
     2521 catcaccggg acgcagggcg cggacgcgtt caccgtgacc gggcgccggg tcgtgctcga
     2581 gggtgcgggc gaggtgaatt acgtaggctt cgaggtcctc gaagtgcgca cgctcggcgg
     2641 cgacgatcgg gtgacgatga ccggcatcga ccccggcacg cgcacgctga tcgacggcgg
     2701 cgagggtcgg gacacgttct tcggccgctt cgacggggac ttcgacggcg agctgacgct
     2761 gctcgagttc gagaagctga cgtttc
//
LOCUS       DASPGN010000565        14438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1158994, whole genome shotgun sequence.
ACCESSION   DASPGN010000565 DASPGN010000000
VERSION     DASPGN010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 14438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14438
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158994"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2053)
                     /locus_tag="VD788_16295"
     CDS             complement(<1..2053)
                     /locus_tag="VD788_16295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HXV77872.1"
                     /translation="MSPVDERPRSDGRSPAFWRSIDELADAPEFRRFVEREFPAYADQ
                     MLTPPTRRNFLKIMGASLALAGMTGCRWPEEEILPFAHRPAGYTPGSTQRFATSIELL
                     GSATGLLVTSYDGRPIKIEGNPESWSSRGATGVWHQASILELYDPERSQHVVRRGSGP
                     QSRGEFLAFARDQFDELRDGGGRGLRVLAAPGSSPSREQMRQRLAMRLPDSRWYEYSP
                     VSRDTIREGARLALGRPVRTHLSLDRAETILSIDEDFLTFHPAAVRYARDFAAGRDPH
                     GGFARLWVVESRFSLTGGMADRRIPLKPSAIPAYVTCLAAAVLAQPGVSLPSGASGLV
                     STLAAARNSPAFAAFDLRVAEELARHRGRSIVTAGPSQPPEVHALVHVINSALSNVGQ
                     TVYYTQEPEPDRIPHPEAIRELAEEIVGGRVDTLVILDGNPAYDAPRELELAALIESV
                     PTAIHLSTHFNETSIACSWHVPQAHYLESWGDGRDYRGGVGIRQPLIRPLFDGRSEIE
                     LLATWIDDEATTGYDIVRRTFFGASAGRDEKSWANALHDGYVADSEWSPLAELPIRAE
                     WGARLERHLVAPESAAGRLELTCVEDSKVLDGRFANNGWLQEMPDPLTKLTWDNAALV
                     APATVAKLGARQGGMIRLAAAERVVEPALVKRSAAHEKLFVAAPSEKLTKEEAARKVL
                     AT"
     gene            complement(2041..2706)
                     /locus_tag="VD788_16300"
     CDS             complement(2041..2706)
                     /locus_tag="VD788_16300"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXV77873.1"
                     /translation="MQNQVQRPLIFPKWVDGLRPVAALLLLGGPVYALALLYYGGSPK
                     ATDVGYSPEQPVAYSHALHAGQLGIDCRYCHTTVEGNAFAAIPPTETCMNCHTNIYPQ
                     SPKLVAVRESYSTGLPVRWVKVHDMPDYVYFDHSAHVSRGVGCVECHGRVDKMEVVYQ
                     VETLSMGFCLDCHRDPDPRLRPVSEVTNMAWEPAGDAREFGGRVRQQYGLDPSEDCST
                     CHR"
     gene            complement(2867..3244)
                     /locus_tag="VD788_16305"
     CDS             complement(2867..3244)
                     /locus_tag="VD788_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77874.1"
                     /translation="MSAPTPTGIRLLVLLLFVSPLIQTWAASTGRRFLTLGGTSWGSW
                     LSWCFAAPLVALLLLRRDGRARFSCYLLLTVFLWRGFRAMSPAAIVLALSIVAYLQLP
                     AVRRVYPRIDSESLIARLRRLRH"
     gene            complement(3241..4746)
                     /gene="hemG"
                     /locus_tag="VD788_16310"
     CDS             complement(3241..4746)
                     /gene="hemG"
                     /locus_tag="VD788_16310"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HXV77875.1"
                     /translation="MIRRPYPIDLPAHCRVAIVGGGISGLAAAHRLVETGSVRGTELV
                     LVEREDRIGGQVRTLRTDPYLLEAGPDTMVTQKPAGLALCRRLGLEPELIRPYRGEPT
                     VHVLRHGRVHRVPDGFSLVGPTRLAPLLASGLFGPAAKIRMIAERFLPPVPPAEGDES
                     LRSFVTRRYGRAAFERVAEPIVGGLFTADADRLSMQLTLPRFLDLEAQSGSVWRGLSN
                     ARNGTTGSAAHEPVALRGGMQQLVDRLEQRLTGVTIATRTAARSLARADAGGWLLELE
                     TGQVLRAEAVVLACPGFVAARLLRSLAPRLADRFGELDYASCATINLVYRRGDVADRH
                     EGFGFFVPRTEGIPLLACSYVSRKFPDRAPADRLVLRAYVGGALHAELPALEDRILVA
                     RVHALLQSLLGITRPPSFARVHRHPQAMPQYVVGDLARVRDIERLLADQRGLFVCGGS
                     LDAIGLPDCIRSGERAADRVDQHLERRPRARRIDRSGSGPALRPALNAGPR"
     gene            complement(4743..5852)
                     /gene="hemH"
                     /locus_tag="VD788_16315"
     CDS             complement(4743..5852)
                     /gene="hemH"
                     /locus_tag="VD788_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HXV77876.1"
                     /translation="MEVKHLSRHDDRRTGLLLINLGTPDSYRPRDVRRYLREFLSDPR
                     VLDIPSWKRLLVLHLLILPFRPHASGKAYAKIWTAHGSPLLVHGLALRDRVRDRLGPQ
                     AIVELGMRYGRPSIAQALETICRRGAERIVVFPLYPQYSSAATGSSLERVYQEVGRRV
                     NAPCVEIVPPFYDHSAFIDACAGVARPIVEAADAEMVFFSFHGLPERQIHKSDCVGTH
                     CLATADCCVRPSPAHRWCYRAQCEATARRLALALRIAESKRVVCFQSRLGRDPWIRPQ
                     TDVVLAEHARRGVKRAVILSPAFVADCLETLEELGIRARGIWRENGGQVLDVVPCVNS
                     DDRWVEGVVRIAAETSPSLARLTRPTVAGKEAIEA"
     gene            complement(5985..6647)
                     /locus_tag="VD788_16320"
     CDS             complement(5985..6647)
                     /locus_tag="VD788_16320"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXV77877.1"
                     /translation="MSEHEIQDAGPRRSLLHSPYLWAVLAGLILIPAMRPFLRHEPAP
                     PAVQFELPEFRLVDSRGAAFGSDDLDGRVYVADFIFTRCGSICPALTRSMKLLDRRFR
                     ERGVDGVHLVSITVDPTYDTPEVLARYADEHAIDLERWTLLTGPPASVRSLVIDGFRL
                     GLGQAPETGTPPESPAEIAHSGKLVLVDGSGGVRGYYDADGEGLDEVFHRTLHVLKQG
                     SR"
     gene            6828..7475
                     /locus_tag="VD788_16325"
     CDS             6828..7475
                     /locus_tag="VD788_16325"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXV77878.1"
                     /translation="MAQIFPSWTNRLPLFVALAGPLLGILVVGGIWYYFSPRYTDVGY
                     APVQPVPYSHELHVGQLGLDCRYCHASVEVSAVSNIPPTQVCMNCHSLIKRDSPLLEP
                     IRASLTSGRPMRWIRVHMLPDYAFFNHAVHVRAGVGCISCHGKIHEMEVVAQAEPLSM
                     SWCLDCHRNPGPHLRPGEEVTNMEWFPPPDQSAWAMQAIETKNLAPPEDCSACHR"
     gene            7472..10474
                     /locus_tag="VD788_16330"
     CDS             7472..10474
                     /locus_tag="VD788_16330"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-cluster-containing hydrogenase"
                     /protein_id="HXV77879.1"
                     /translation="MKHEHGYWRSLEQLSGSSHESVDEFAAGVAEPPQRVSRREMLSL
                     LGASMSFAGLVACRKPAEAIVPFVQAPENVIPGVPKYYATNMPFGLDAYGLLVESHEG
                     RPTKIEGNRLHPSTHGASNSWIQASILDLYDPDRSQTVRHLGQTGDWQAFVTAWTEME
                     SEYLASGGSGLALLGESFASPTLARLGQAFSARFPHAIRATYEPVGDDTSFAGMELAA
                     GEPYRVMLRLENAEVVLSLDSDFLGVEADAVRNTRGFAAARDPERGSMNRLWVVESNY
                     SLTGANADHRLRLAAADVPAFVSMLTAELRKRAGDLTLPAASSRPLPQVDARWLAALV
                     DDLTHHRGRSLIVAGRRQPAAVHAAVYALNRALGNVGASVSYYEPLDAALPRGLEPLV
                     RAMRDGQVSTLVILGCDPVSNAPADLAFGEAMEQVPVTVHLSSYLDATSVKASWHLNR
                     AHYLESWGDVRAVDGTLGVTQPLIAPLFDGKSPVDVLGLLATGTDAPAYERVRETWKT
                     FLDDGSFEKEWRRVLHDGLLDGSAVVAASPALTIDRVSDALNDAIAVPSDGYEVVFQA
                     SPALFDGRFANNGWLQEVPDALTKLSWDNPALISPHAADALGISNGEMVKITVGQNTI
                     ALPVWIMPGLAERSIVLYLGHRPAGGVGRIASRSGFDVYPLRTTRAMGHAGGATLEAT
                     GTNYALATTQEHGTMNEPNLAPWQKGKPERARDLVRQGTFEQFRRDPEFARRELAFPE
                     RSLWQERRFEQSPQWGMTIDLNACVGCNACVVACQSENNIPIVGKQQVGEGREMHWLR
                     VDRYFTGAADDAAVVFQAVPCMQCENAPCEQVCPVGATVHDEQGLNAMVYNRCIGTRY
                     CSNNCPYKVRRFNYFNFTKDTPESLALAYNPDVTVRSRGVMEKCTYCVQRINEGLIEA
                     KLSGRPLSDGEIRTACQQACPATAIEFGDIRDDSSRVSRSKLNPRNYVLLEELFTKPR
                     TTFLAKLRNPHPELAQADLDPTPSEGHH"
     gene            10474..11079
                     /locus_tag="VD788_16335"
     CDS             10474..11079
                     /locus_tag="VD788_16335"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXV77880.1"
                     /translation="MPRASWPAMAMVLLALVATGCVRGCNSSRPPIHLNPNMDNQPRY
                     DPQQASDFFYDGKGMRAPVEGTVARGELQQDRPFHTGTDSAGNFLTEIPPLTPAQLAR
                     GPERYAIYCEPCHDPRGLGQGILYEYGGVPTASFHDEQRSAYPPGRLFDVITNGSGLM
                     QGYRYPIPPADRWAIVAHVQQLQRARQDRAHATSAPPEGTR"
     gene            11076..12968
                     /locus_tag="VD788_16340"
     CDS             11076..12968
                     /locus_tag="VD788_16340"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HXV77881.1"
                     /translation="MKGRLVVIATIIGLALGAGAMLRLGTYLVSTEYRSPARAAGDAP
                     TPRMQGIADERAEAEAQALAKPYEEEAYRAFPVVGSRVAVWVVAELHLLFAAFVLAVP
                     MFAFLIELIGYITGDKRYDRLAYEFCKLLSTSFSFTASLGAFLLFMLIGLYPKLMTYL
                     GHVFSPTFLPYVGLFLFEALFLYSYYYGWGKFNPKFHLFLGLMLNLVGTAIMFIANAW
                     LTFMTSPAGISETGARISTWEAVSNYTWMPINIHRIIANVAFGGSVAAAYAAFKFLYA
                     KSDDERAHYDWMGYIGNFVAISAFLPLPFAGYWLAKEIYGFSQTMGLQMMGGAFSWLF
                     IIQAALIGNLFLAANFYLWLGMGRIEGAGQYQRFIKYLLIAIALCFAVWATPRSIIST
                     VSEIRAMGGSSHPALGFLGVMSAKNTAVNILILSTYISFLLYRRTGKIATVSWYKLGN
                     TLQFAIFAAAAGIVIFYGIYGYFVEAAVRIGFSVPQVLSVLIAMVLITAIDIPLYRNA
                     KKTGETIWGRIPAISQYTLIFIAVSFTWLMGLMGYVRSGLRQHWHVYGVMRDTSVDAF
                     TPTLGFATRVVSVTVLIFFAFIGLVFWLASLPGSKDWEPRTAPVKQPAPDGVVQPTMA
                     RERVGT"
     gene            12975..13472
                     /locus_tag="VD788_16345"
     CDS             12975..13472
                     /locus_tag="VD788_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXV77882.1"
                     /translation="MNNLRSPTWFNVLLLVLGSTALYTYIGQLVPQKEVLPPEETVMR
                     ADMTTDDLVQVGREIAEGKGLCLTCHTIGKSGALRFPDLEGIAARASDRIPELNALEY
                     MAHSIYKPDEFIVPGFNPGMPVINKPPIGLNDQEILAVLAYLQSLGGTPDVTLETTYA
                     ELGLE"
     gene            13475..14305
                     /locus_tag="VD788_16350"
     CDS             13475..14305
                     /locus_tag="VD788_16350"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXV77883.1"
                     /translation="MVFWGFNLVVIALIVAAMIGLRFLRPGMLVWAIAWWFAWYAFID
                     LGFATPVPASVKKLYMAIVTLALFAYVTSDRGRLDSFMGPIVRLVTVPRYRPLLVAVL
                     LVIPLLAAWNVYAEINVAIEAPAFGRTVHPAPPDKITVHEEEIDLVTADNPYRALEET
                     DPDAFAAHVENGRRLYFENCFFCHGDSMGGDGMFAHALNPIPSNFNDPGVLPMLQESF
                     VFWRVSKGAPGLPEEGGPWDSAMPAWENFLTTEEMWDVVLFLYDFNDYRPRAREEGHH
                     "
BASE COUNT         2368 a         4852 c         4891 g         2327 t
ORIGIN      
        1 aggttgccag caccttgcgg gcggcctcct ccttcgtcag cttctcggag ggcgcggcga
       61 cgaacagctt ctcgtgcgcg gcggagcgct tgacgagggc gggctcgacg acgcgctcgg
      121 cggcggcgag acggatcatc ccgccctggc gcgcacccaa cttggcgacg gtggccggcg
      181 cgaccaacgc ggcgttgtcc caggtcagct tggtcagcgg gtcgggcatc tcctgcagcc
      241 agccgttgtt ggcgaaccga ccgtcgagga ccttcgagtc ctcgacgcag gtcagctcga
      301 gccgtccggc ggccgactcc ggcgcaacga gatggcgctc gagtcgcgcg ccccactcgg
      361 cacgaatggg cagctccgcc agcggcgacc actcgctgtc ggccacgtat ccgtcgtgca
      421 gggcgttggc ccagcttttc tcgtcccgtc ccgcggaagc gccgaagaac gtccgtcgca
      481 cgatgtcgta gcccgtggtc gcctcatcgt cgatccacgt cgcgagcaac tcgatctcgc
      541 tgcgaccgtc gaacagcggt cggatcagcg gttggcggat tccgacgccc ccgcggtagt
      601 cgcggccgtc accccagctc tcgaggtagt gcgcctgtgg cacgtgccag ctgcaggcga
      661 tcgacgtttc gttgaagtgg gtcgacagat ggatcgctgt cggcaccgac tcgatcaacg
      721 cggccagctc gagctcgcgc ggcgcgtcgt aggccgggtt gccgtcgagg atcaccagcg
      781 tgtcgactcg cccgccgacg atctcctcgg ccagctcgcg gatcgcctcc gggtgcggga
      841 tgcgatcggg ttccggctcc tgcgtgtaat acaccgtctg gcccacgttg gacagcgcgc
      901 tgttgatcac gtggaccaga gcgtgcactt cgggcggctg gctcggaccg gcggtgacga
      961 tcgatcggcc gcggtgacgc gccagctcct cggcaacgcg caggtcgaac gcggcgaacg
     1021 ccgggctgtt gcgcgccgcg gccaaggtcg agaccaatcc cgacgcgccg gacggcagcg
     1081 agacacccgg ctgtgccagc accgccgccg ccagacaggt cacgtaggcc gggatcgcgc
     1141 tcggcttcag cggaatccgt cgatcggcca tcccgccggt gaggctgaaa cgactttcga
     1201 cgacccacaa ccgagcgaaa cctccatgcg ggtcccgacc ggcggcaaag tcccgggcgt
     1261 agcgcacggc ggccgggtga aacgtgagaa aatcctcgtc gatcgacagg atcgtttcgg
     1321 cgcggtcgag cgagaggtgg gtgcggaccg gccggcccag cgcgagccgg gcgccctcgc
     1381 ggatcgtgtc acgcgaaacc gggctgtatt cgtaccagcg ggaatccggc aagcgcatcg
     1441 cgagtcgctg ccgcatctgc tcgcgtgacg gcgaggaacc aggggccgcc agcacgcgca
     1501 gcccgcggcc gccgccgtcg cgcagctcgt cgaactggtc gcgagcgaag gcgagaaact
     1561 cgccccggct ctgcggaccc gacccgcgcc gaacgacgtg ctggctgcgc tccggatcgt
     1621 acagctcgag gatgctcgcc tggtgccaga cgccggtcgc gccgcgactg gaccagctct
     1681 ccgggttgcc ctcgatcttg atcggccgcc cgtcgtagct cgtcaccagc aggccggtgg
     1741 ccgatccgag cagctcgatc gacgtcgcga accgctgggt cgatcccggc gtgtaccccg
     1801 ccggccggtg ggcgaacggc aggatctcct cctcgggcca gcggcagccg gtcatcccgg
     1861 ccagcgccag cgaggcgccc atgatcttga ggaagttgcg acgggtcggc ggcgtgagca
     1921 tctggtccgc gtaggccggg aactcacgct cgacgaagcg acggaactcc ggagcatccg
     1981 ccagctcgtc gatgctgcgc cagaacgcgg gcgagcggcc gtccgagcgg ggtctctcgt
     2041 ctaccggtga catgtgctgc aatcctcgga agggtcgaga ccgtactgtt gtctgactcg
     2101 cccgccgaac tcgcgtgcgt cccctgccgg ttcccacgcc atgttggtca cctcgctgac
     2161 cgggcgcagg cgtggatccg gatcccggtg gcagtcgagg cagaagccca tgctcagcgt
     2221 ttcgacctgg tacaccacct ccatcttgtc cacccgaccg tggcactcga cacagcccac
     2281 gccgcgcgag acgtgagcgc tgtgatcgaa gtagacgtag tccggcatgt cgtggacctt
     2341 gacccaccgc accggcaagc cggtggagta gctctcgcgc accgcgacca gcttggggct
     2401 ctgcgggtag atgttcgtgt ggcagttcat gcacgtctcg gtcggtggga tcgcggcgaa
     2461 ggcgttgccc tccaccgtcg tgtgacagta gcgacagtcg atgcccaatt gtccggcgtg
     2521 caacgcgtga ctgtaggcga cgggctgctc ggggctgtac ccgacatccg tcgctttcgg
     2581 cgagcctccg tagtagagaa gggcgagcgc gtagaccgga ccgccgagca gcagtagcgc
     2641 ggcgaccgga cggaggccat ctacccactt cgggaagatg aggggtcgct gaacctggtt
     2701 ctgcatggcg gaagacccgg tatcatagcg agcgaccctg gggtccgcaa gacaacgaaa
     2761 ccggccgcgc gcgacgtcgg gtcggtcgtt ccagcgcgcg atatcagtcc aacgcaggcg
     2821 ggtcgattcg cgccgcggca agcgcgccgg cgagccggat cgacggctag tggcgcaacc
     2881 ggcgaagccg cgcgatcaga ctctcggaat cgatccgcgg atagacccgc cgtacggcgg
     2941 gtagctgcag ataggccacg atcgacagcg cgagcacgat cgccgccggg ctcatcgcgc
     3001 ggaacccgcg ccagaggaaa acggtcagca ggagatagca ggagaatcgg gcacggccgt
     3061 cgcgacggag cagcaagagc gcgaccagcg gggcggcgaa gcaccagctg agccaactgc
     3121 cccacgacgt gccacccaag gtgagaaagc gtcgcccggt cgacgccgcc cacgtctgaa
     3181 tcagcgggct cacgaacaac agcagcacga gcaggcgaat gccggtcggg gtcggtgcac
     3241 tcacctcggt ccggcattca gcgccgggcg gagcgctgga ccgctccccg accggtcgat
     3301 gcgacgcgcg cgcggccggc gctcgagatg ctgatcgacg cgatccgccg cacgttctcc
     3361 cgagcgaatg cagtccggga gcccgatcgc gtccaggctc ccgccgcaga cgaacaatcc
     3421 acgctgatcg gccagcaacc gttcgatgtc gcgaacgcgt gccagatcgc cgacgacgta
     3481 ttgcggcatc gcctgcggat gtcggtgcac gcgcgcgaag ctgggtggtc gcgtgatgcc
     3541 gagcagcgat tgcaggagtg cgtgcacgcg tgcgacgagg atccgatcct cgagtgccgg
     3601 cagttcggcg tgcaacgcgc caccgacgta ggcgcggagg accagccgat ccgccggcgc
     3661 gcggtcgggg aacttgcggc tgacgtagct gcaggcgagc agcgggattc cctcggtgcg
     3721 tggaacgaag aatccgaagc cttcatggcg atcggcaacg tcgccgcgtc ggtagacgag
     3781 gttgatcgtg gcacacgacg cgtaatcgag ttccccgaac cggtcggcga gccgcggcgc
     3841 gagcgagcgg agcagtcggg cggccacgaa ccccggacag gcgagaacga cggcctcggc
     3901 tcgcaacacc tgtcccgtct cgagctcgag caaccagccg ccggcgtctg cgcgagcgag
     3961 actgcgggcc gcggttcggg tcgcgatcgt cacgccggtc agccgctgtt ccaggcgatc
     4021 gacgagctgc tgcattccac cgcgcagcgc gacaggctcg tgcgccgcgc tgccggtcgt
     4081 tccgttccgt gcgttgctca gaccgcgcca aacgctgccg gattgcgctt cgagatcgag
     4141 gaagcggggc agcgtcagct gcatgctcag ccgatcggcg tcggccgtga acaatccacc
     4201 gacgatcggt tcggcgaccc gctcgaacgc cgcgcgcccg tagcgtcggg tgacgaacga
     4261 tcgcaagctc tcgtcaccct cggctggtgg caccggcggc aggaaccgtt cggcgatcat
     4321 gcggatcttg gccgccggcc cgaacaaccc cgacgcgagc agcggggcca gccgcgtcgg
     4381 cccgacgagc gagaagccat ccgggacgcg gtgcacccgt ccgtgacgca ggacgtggac
     4441 ggtgggctcg ccccggtacg ggcggatcag ctccggttcg aggccgagcc gccgacacaa
     4501 cgccaggccg gcgggtttct gcgtcaccat cgtgtccgga ccggcctcca gtaaataagg
     4561 atcggtgcgc agcgtgcgga cctgcccgcc gattcgatcc tcgcgctcga ccagtacgag
     4621 ctcggtgccc cgaaccgagc cggtctcgac caggcggtgt gccgcggcga gaccggagat
     4681 gccgccgccg acgattgcga cgcggcagtg cgccggaaga tcgatcgggt acggtcgccg
     4741 tatcacgctt cgatcgcctc cttcccggcg accgtcgggc gggtaagtcg ggcgagcgac
     4801 gggctcgtct cggcggcgat ccgcacgacg ccctcgaccc agcggtcgtc gctgttgacg
     4861 cacggaacga cgtccagcac ctgcccaccg ttctcgcgcc agattccgcg ggcgcggatt
     4921 ccgagctctt cgagcgtctc gaggcagtcg gcgacgaacg ccgggctgag gatcaccgcg
     4981 cgcttgactc cgcgccgcgc gtgctccgcg agcacgacgt ccgtctgggg ccggatccag
     5041 ggatcgcgcc cgaggcggga ctggaagcag accacacgct tcgattccgc aatccgcaat
     5101 gccagcgcca gtcggcgggc ggtcgcctcg cactgcgcgc ggtagcacca gcgatgcgcc
     5161 ggcgacggtc ggacgcaaca gtcggcggtg gccagacagt gcgttccgac gcagtcggac
     5221 ttgtggatct gtcgctcggg gaggccgtgg aagctgaaga acaccatctc ggcatcggcg
     5281 gcctcgacga tcggccgcgc gacgccggcg cacgcgtcga tgaacgccga gtgatcgtag
     5341 aacggcggca cgatctcgac gcagggagcg ttgacgcgtc ggccgacctc ttggtagacg
     5401 cgctccaggc tcgagccggt ggccgccgag ctgtattgcg gatacagcgg gaagacgacg
     5461 atccgttccg cgccccggcg gcagatcgtc tcgagcgctt gagcgatcga gggtcggccg
     5521 taacgcatcc cgagctcgac gatcgcttgg ggtcccagcc ggtccctgac gcggtctctc
     5581 agcgccaggc cgtgaaccaa gagcggcgaa ccgtgggcgg tccagatctt ggcgtaagcc
     5641 ttgccggacg cgtgcggacg gaacggcagg atgagcaggt ggagcaccag caggcgtttc
     5701 cacgacggga tgtcgagtac ccgcggatcg ctgagaaact cccgcaggta tcgcctgacg
     5761 tctcgcggcc ggtagctgtc cggggttccg agattgatca gcagcaggcc ggtcctgcgg
     5821 tcgtcgtgtc gtgaaaggtg tttgacttcc acgctccgca acgcgtaacc cccagcagac
     5881 gagacgtcgc ctcgtacacg acaaccccgg ctatccgccg cacccggatc cgatcggacc
     5941 cggcattgta ccagggtccg acgacgccca acacggcgag tcggtcagcg ggacccctgc
     6001 ttcagcacgt gcagcgtgcg gtgaaacacc tcgtcgagcc cttcaccgtc ggcgtcgtag
     6061 tatccgcgaa ccccaccgct gccgtcgacg agcaccagct ttccgctgtg cgcgatctcc
     6121 gccggcgact ccggtggcgt accggtctcg ggggcctggc ccagacccag gcggaatccg
     6181 tcgatgacca acgagcgcac cgacgcgggc ggaccggtca gcaaagtcca gcgttcgagg
     6241 tcgatcgcat gctcgtcggc gtaccgcgcc aggacctccg gggtgtcgta ggtcgggtcg
     6301 acggtgatgc tcaccagatg gacgccgtcc actcctcgct cgcggaagcg ccggtcgagc
     6361 agtttcatcg agcgggtgag cgcgggacag atcgatccgc aacgcgtgaa gatgaagtcc
     6421 gccacgtaga cccgcccgtc gagatcgtcg ctgccgaacg cggcgcctcg actgtcgacc
     6481 agtcgaaact ccggcagctc gaactgaacg gcgggcggtg cgggttcgtg acgcaggaac
     6541 ggccgcatcg ccggaatgag aatcagtcct gccaacacgg cccacaagta agggctgtgc
     6601 agcagcgagc gacgcggtcc cgcgtcctgg atctcgtgct cgctcactcg cgaatgatgt
     6661 cacacccgag ccgcggcgac cagccgcgga cgatcgaagg gaatcaagca aaacgaggac
     6721 cgtaacggtc ttgaattcga cttgaccgac ccgtagaatc aggggccgcg ggtcgcggcg
     6781 ccggtttcga ccagaggcca cacggcgccc cgagggagcg aggtgtcgtg gctcagatct
     6841 tccccagctg gaccaaccgc ctgcccttgt tcgtcgctct cgccggtccg ctcctcggga
     6901 tcctcgtggt cggtggcatc tggtactact tctcgccgcg ctacaccgac gtcggctacg
     6961 cgccggtgca gccggttccc tacagccacg agcttcacgt cggtcagctc gggctggact
     7021 gtcgctactg tcacgcctcc gtcgaggttt ccgccgtctc gaacatcccg ccgacccagg
     7081 tgtgcatgaa ctgtcacagc ctgatcaagc gggacagccc gctgctcgaa ccgatccggg
     7141 ccagcctcac gagcggacgc ccgatgcggt ggatccgcgt ccacatgctt cccgactacg
     7201 ccttcttcaa ccacgcggtc cacgtccgcg cgggagtggg ttgcatcagc tgtcacggga
     7261 agatccacga gatggaggtg gtcgcgcagg ccgaaccgct cagcatgagc tggtgcctgg
     7321 actgccatcg gaaccccggc cctcacctgc gccccggcga agaggtgacg aacatggagt
     7381 ggttcccccc gccggaccag tcggcgtggg cgatgcaggc gatcgaaacg aagaacctcg
     7441 ccccaccgga ggactgttcg gcgtgtcatc gatgaaacac gagcacggtt actggcggag
     7501 tctcgagcag ctctccggca gttcgcacga gtcggtcgac gagttcgccg cgggcgtggc
     7561 cgagcccccg cagcgggtca gccggcgcga gatgctgtcg ctgctgggtg cgtcgatgtc
     7621 gttcgccggt ctcgtcgcct gccgcaagcc ggccgaagcg atcgtgccct tcgtccaggc
     7681 cccggagaac gtcataccgg gcgtgcccaa gtactacgcg acgaacatgc cgttcggact
     7741 cgacgcctat gggctgctgg tcgagagcca cgagggacgt ccgaccaaga tcgaggggaa
     7801 tcgccttcat cccagcaccc acggggcgtc gaactcgtgg atccaggcgt cgatcctcga
     7861 cctctacgac ccggaccgct cgcaaaccgt gcgccatctc gggcagaccg gcgactggca
     7921 ggcgttcgtc acggcgtgga ccgagatgga gagcgagtac ctggcttccg gcgggtcggg
     7981 gctcgccctg ctcggcgagt cgttcgcgtc gccgacgctc gcgcgtctcg gccaggcctt
     8041 ctccgcgcga ttcccgcacg cgatccgcgc gacctacgag ccggtgggtg acgacacctc
     8101 gttcgcggga atggagctcg ccgccggtga gccgtaccgc gtgatgctcc ggctcgagaa
     8161 cgccgaggtc gtactgtcgc tcgactcgga cttcctcggg gtcgaggccg acgccgtccg
     8221 caacacgcgt ggcttcgcgg cggcccgtga cccggagcgg ggatcgatga accggctgtg
     8281 ggtcgtcgag tcgaattact cgctgaccgg agcgaacgcc gaccaccggc tgcggctcgc
     8341 cgcggcggac gtcccggcgt ttgtttcgat gctgaccgcc gagctgcgga aacgcgccgg
     8401 cgatctcacg ctcccggccg cctcctcgcg ccccctgccc caggtcgacg cgcgctggct
     8461 cgccgcgctc gtcgacgact tgacccacca tcgcggccgt tcgttgatcg tcgccggtcg
     8521 gcgtcagccg gcggcggtgc acgcggcggt ctacgcgctg aaccgggcgc tcggcaacgt
     8581 cggcgccagc gtgtcgtact acgagccgct cgacgcggcg ctcccgcgcg gactcgagcc
     8641 gctcgtacgg gcgatgcgcg acggccaggt ttcgacgctg gtgatcctag gctgcgatcc
     8701 ggtgagcaat gcaccggccg acctcgcgtt cggcgaggcg atggagcagg tcccggtcac
     8761 ggtccacctc agttcctacc tcgacgccac ctcggtgaaa gcctcgtggc acctcaaccg
     8821 cgcgcactac ctcgagtcgt ggggggacgt ccgcgcggtc gacgggacgc tgggcgttac
     8881 gcagccgctg atcgcgccgt tgttcgacgg caagagtccg gtcgacgtgc tcggactgct
     8941 cgcgaccggc acggacgctc cggcctacga acgcgtccgc gagacgtgga agacgttcct
     9001 cgacgatggc tcgttcgaga aagaatggcg ccgcgtgctg catgacgggc tgctcgacgg
     9061 gagcgcggtc gtggcggcgt cgcctgcgtt gacgatcgac cgggtgagcg acgcgttgaa
     9121 cgacgcgatc gcggtgccgt ccgacggata cgaggtcgta ttccaggcct ctcccgcatt
     9181 gttcgacggt cgcttcgcca acaacggctg gctgcaggag gtcccggacg cgctgaccaa
     9241 gctgagctgg gacaacccgg cgctgatctc gccgcacgcc gccgacgcgc tggggatcag
     9301 caacggcgag atggtgaaga tcaccgtggg ccagaacacg atcgcgttgc cggtgtggat
     9361 catgccgggt cttgccgagc gcagcatcgt gctctacctg ggtcaccgtc cggcgggcgg
     9421 ggtcggtcga atcgcttcgc ggtcggggtt cgacgtctat ccgctgcgca cgacgcgcgc
     9481 gatggggcac gccggcggcg cgacgctcga agccacgggg acgaactacg cgttggccac
     9541 gacccaagag cacggcacga tgaacgaacc caacctggcg ccgtggcaga aaggaaagcc
     9601 ggagcgcgcg cgggatctgg ttcggcaggg cacgttcgag cagttccgcc gggacccgga
     9661 gttcgcccgg cgcgagctgg ccttcccgga gcggtcgctg tggcaggagc ggcggttcga
     9721 gcagtccccg cagtggggca tgacgatcga cctgaacgct tgcgtcggat gcaacgcgtg
     9781 cgtcgtcgcg tgccagagcg agaacaacat cccgatcgtc ggcaagcagc aggtcggtga
     9841 gggtcgcgag atgcactggt tgcgcgtcga ccgctacttc accggggcgg cggacgacgc
     9901 cgcggtcgtg ttccaggcgg taccgtgcat gcagtgcgag aacgcgccgt gcgagcaagt
     9961 ctgtcccgtc ggcgcgacgg tccacgacga acaggggctg aacgccatgg tctacaaccg
    10021 gtgtatcggc acccgctact gctcgaacaa ctgcccgtac aaggtgcgac ggttcaacta
    10081 cttcaacttc accaaggaca cgcccgaatc gctcgcgctg gcctacaacc ccgacgtcac
    10141 cgtgcgcagc cggggcgtga tggaaaagtg cacttactgc gtgcaacgga tcaacgaggg
    10201 gctgatcgag gcgaaactgt ccggccgccc gctgagcgac ggcgagatcc ggaccgcctg
    10261 tcagcaggcc tgcccggcga cggcgatcga gttcggcgac atccgcgacg actcgagcag
    10321 ggtcagccgc tccaagctca acccgcggaa ctacgtgctg ctcgaggagc tgttcaccaa
    10381 gccgcgcacg acgttcctgg ccaagctgcg caacccgcat cccgagctgg cccaggcgga
    10441 cctcgatccc acgccgagtg agggccatca ctgatgccac gggcgagttg gccggcgatg
    10501 gcgatggtcc tgctcgctct ggtcgcgacc ggctgcgtgc gcggctgcaa ctcctcgcgc
    10561 ccgccgatcc acctcaatcc caacatggac aaccagccgc gttacgaccc gcagcaggca
    10621 agtgatttct tctacgacgg caagggaatg cgggccccgg tcgagggtac cgtcgcgcgc
    10681 ggcgagcttc aacaggatcg gccgtttcac accggcaccg attccgccgg gaatttcctg
    10741 acggagatcc cgccgctcac tccggcgcag ctcgcgcggg gtcccgagcg atacgcgatc
    10801 tactgcgagc cgtgtcacga tccccgcgga ctcggacagg gcatcctcta cgagtacggc
    10861 ggcgtgccga ccgcctcgtt tcacgacgag cagcgatcgg cctacccgcc tggccggcta
    10921 ttcgacgtga tcaccaacgg atccggtctg atgcagggat accgttaccc gatcccgccg
    10981 gccgatcgct gggcgatcgt cgcgcacgtc cagcagttgc agcgtgcccg gcaggaccgg
    11041 gcgcacgcga cgtccgcgcc gccggagggc acccgatgaa gggcaggctg gtcgtgatcg
    11101 cgacgatcat cggtctcgcg ctcggcgcgg gtgcgatgtt gaggctcggc acctacctgg
    11161 tctcgacgga gtaccgttcg ccggcccggg ccgcgggcga cgccccgaca ccacggatgc
    11221 agggcatcgc cgacgagcgc gccgaggccg aggcgcaggc gctggccaag ccctacgagg
    11281 aagaggcata ccgcgccttt cccgtcgtcg gcagtcgcgt cgcggtctgg gtcgtcgccg
    11341 agcttcacct gctgtttgcc gcgttcgtcc tcgccgtccc gatgttcgcc ttcttgatcg
    11401 agttgatcgg gtacatcacg ggtgacaagc gatacgatcg gctggcctac gagttttgca
    11461 agctgctcag cacgtcgttc tcgttcaccg ccagcctcgg cgcctttctg ctgttcatgc
    11521 tgatcggtct gtaccccaag ctgatgacct atctcggtca cgtcttctcc ccgacgttcc
    11581 tgccgtacgt cgggttgttc ttgttcgagg cgctgtttct ctacagctac tactacggtt
    11641 ggggtaagtt caatcccaag ttccatctgt tcctcgggct gatgctcaac ctggtcggca
    11701 ccgcgatcat gttcatcgcc aacgcctggc tgaccttcat gacttctccg gcaggcattt
    11761 ccgagaccgg cgcgcgaatc agcacgtggg aggcggtcag caactacacg tggatgccga
    11821 tcaacatcca ccgcatcatc gccaacgtgg ccttcggcgg ttcggtcgcc gcggcctacg
    11881 ccgccttcaa gtttctctac gccaagtccg acgacgagcg cgcgcactac gactggatgg
    11941 gctacatcgg gaatttcgtc gcgatcagcg cgttcctccc gctgccgttc gccggctact
    12001 ggctggccaa ggagatctac ggcttctcgc agacgatggg gctgcagatg atgggcggcg
    12061 cgttctcctg gctgttcatc attcaagcgg cgctgatcgg caacctgttc ctggccgcca
    12121 acttctacct gtggctgggc atgggacgga tcgagggggc cggccaatac cagaggttca
    12181 tcaagtatct gctgatcgcg atcgcgctgt gcttcgccgt gtgggcgacc ccgcgctcga
    12241 tcatctcgac cgtgtccgag atccgcgcga tgggcggctc ttcgcatccc gcactcggct
    12301 ttctcggcgt gatgtcggcc aagaacacgg cggtcaacat cctgatcctc agcacctaca
    12361 tcagcttcct gctctatcgg cggaccggga agatcgcgac cgtctcgtgg tacaagctgg
    12421 gcaacacgct gcagttcgcg atcttcgccg cggccgccgg catcgtcatc ttctacggca
    12481 tctacggcta tttcgtcgag gcggcggtcc gcatcggctt ctccgtcccg caagtgctgt
    12541 cggtgttgat cgcgatggtg ttgatcacgg cgatcgacat tccgctctac cgcaacgcga
    12601 agaagacggg tgagacgatc tggggtcgca tcccggcgat ctcgcaatac acgctgatct
    12661 tcatcgccgt cagcttcacc tggctgatgg ggttgatggg ctatgtgcgg tcgggactca
    12721 gacagcactg gcacgtctac ggcgtgatgc gcgacacgtc ggtcgacgcc ttcacgccga
    12781 cgctcgggtt cgccacgcgg gtggtgtcgg tgacggtgct gatcttcttc gccttcatcg
    12841 gcctggtgtt ctggctggcc agtttgccgg gatcgaagga ctgggagcca cggacggcgc
    12901 cggtcaagca gccggcgccc gacggggtgg tacaaccgac gatggcacgc gagcgcgtgg
    12961 ggacctagac ccggatgaac aacctcagat ccccgacgtg gttcaacgtg ttgctgctcg
    13021 tgctgggctc gacggcactg tacacctaca tcgggcagct cgtaccgcaa aaggaggtcc
    13081 tgccgcccga ggagacggtg atgcgcgccg acatgacgac ggacgacctg gttcaggtcg
    13141 gacgcgagat cgccgagggc aaggggctgt gcctgacctg tcacacgatc ggcaagagcg
    13201 gcgccctgcg ctttcccgat ctcgagggga ttgcggcccg tgcctccgac cggatcccgg
    13261 agctgaacgc gctggagtac atggcgcatt cgatctataa gcccgacgag ttcatcgtac
    13321 cggggttcaa tcccgggatg ccggtgatca acaaaccgcc gatcgggctg aacgaccaag
    13381 agatcctcgc ggtactggcc tatctgcaga gtctgggtgg aacgcccgac gtgacgctgg
    13441 aaacgactta cgcggagctc gggctggagt agaaatggtc ttctggggct tcaacctggt
    13501 ggtgatcgcg ctgatcgtcg cggcgatgat cggtctgcgt ttcctgagac cggggatgct
    13561 ggtctgggcg atcgcgtggt ggttcgcgtg gtacgccttc atcgacctcg gattcgcgac
    13621 accggtcccc gcgtcggtga agaagctgta catggcgatc gtcacgctcg cgttgtttgc
    13681 ctacgtcacc tcggatcgag gacggctcga cagcttcatg ggcccgatcg tgcggctcgt
    13741 caccgtaccg cgctatcgtc cgctgctcgt cgccgtgctg ctcgtgatcc cgctgctggc
    13801 cgcctggaac gtctacgcgg agatcaacgt ggcgatcgag gcaccggcgt tcggcaggac
    13861 cgttcacccg gccccgcccg acaagatcac ggttcacgag gaggagatcg acctcgtgac
    13921 ggccgacaac ccgtaccgcg cgctggagga gaccgacccc gacgcgttcg ccgcgcacgt
    13981 cgagaacgga cgccggctgt acttcgagaa ctgcttcttc tgtcacgggg actcgatggg
    14041 cggcgacggt atgttcgcgc atgcgctgaa cccgattccg tcgaacttca acgacccggg
    14101 cgtgttgccg atgctgcagg agtcgttcgt cttttggcgg gtttccaagg gcgcgccggg
    14161 tctgccggag gaaggcgggc cgtgggattc ggcgatgccg gcctgggaga acttcctcac
    14221 cacggaagag atgtgggacg tggtgctgtt cctctacgac ttcaacgact accggccgcg
    14281 ggcccgtgag gaggggcatc actgatgcgg cgcgggttgg cggggctgat gctgctcgcg
    14341 ttcgccggtg gaccgggggc ggcacagtcc gacctcggca gcgacccgca acgggcggcg
    14401 gggaaggagc tgtacgacaa gtactgcgcg cagtgcca
//
LOCUS       DASPGN010000566         6944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1041713, whole genome shotgun sequence.
ACCESSION   DASPGN010000566 DASPGN010000000
VERSION     DASPGN010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041713"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(563..1711)
                     /gene="mutY"
                     /locus_tag="VD788_16355"
     CDS             complement(563..1711)
                     /gene="mutY"
                     /locus_tag="VD788_16355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HXV77884.1"
                     /translation="MRRALLKWYDDHRRDLPWRRTSDPYAIWVSEVMLQQTRAATVIP
                     YYRRFIRRFPDPFALAEATEDEVLSAWTGLGYYRRARMLRAGARAIVERHGGRLPDDP
                     AVLIELPGIGRYTAGAIASIAYGKPAAVLDGNVRRVLARLLAIDGAVCGPAEERRRLW
                     SAAGELVRGARPGDVNQALMELGALVCTPANPDCTDCPLRRRCRAASAGNPSGYPSAS
                     RRAAALDDDAAVLWLEHRGKLLLAGRTADTPLRGNWDLPTRIVRAGVTSGETIRRLMR
                     ERYGLTLAVGARTTELTHGIMKRRLRLLVHRGARRAGRVAAADGLRWVAIERLGETPV
                     SGATRKIVRIVSGFDPFGEAVRPATGERPTGDRGVESGRIGRDRDQGQ"
     gene            complement(1725..2051)
                     /locus_tag="VD788_16360"
     CDS             complement(1725..2051)
                     /locus_tag="VD788_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HXV77885.1"
                     /translation="MPSYERHVFVCENRRDPDNPKGCCAAKGGEEIRSRLKKLAFDAG
                     LKGRVRVNSAGCLGQCATGVTIVVYPESVWYGRVTLDDVDELFREHIVAGRPVERLRL
                     ERIRPD"
     gene            2128..2688
                     /locus_tag="VD788_16365"
     CDS             2128..2688
                     /locus_tag="VD788_16365"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXV77886.1"
                     /translation="MGQWRLLERRVVQRCRVFDVSSATFESVESNRPHQFFVLNAPDW
                     VNVIPITDGGRVLMVRQYRFGIAADTLEIPGGMCDAGESPLTAARRELREETGYRAKR
                     LTGVGWVHPNPPIQDNRCHTFVARGLSQEGPPSATDDERIELIDVALDELPRLLVDGT
                     ITHALVLAAFQLFDGYRRGFSRQDGA"
     gene            complement(2666..3463)
                     /locus_tag="VD788_16370"
     CDS             complement(2666..3463)
                     /locus_tag="VD788_16370"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV77887.1"
                     /translation="MARAIAGSLVLVTGASAGIGRAATRRFVERGARVIGVARAIERL
                     RELSDELGGESRLVPIGADVSDARAMDRLAARVATEIGLPDVIVANAGISLDATFENT
                     HDEALERVFAVNVFGVVRTVRPFVRPMVERGTGRILLISSIVGKRGIPYYSAYSASKF
                     ALHGLADALRAELYGSGVSVGLVCPSSTDSELHDRALRSGPGQRRVRPRRHSAESVAE
                     AIVRMAASDRREVVLSTEAKLLAIGSRLFPALIDRMLARMLRHRPAD"
     gene            complement(3486..4544)
                     /locus_tag="VD788_16375"
     CDS             complement(3486..4544)
                     /locus_tag="VD788_16375"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXV77888.1"
                     /translation="MGVPPFNAYLGTEIVRRGKGQAEATLELQPHHLNRRGVAHGGVV
                     TALLDSALGAAVISDIPQEWWCATTSLTAHFIGGARGGRLVATGRVLRRGRSVAFASG
                     EIHDERGTLVAAGSGTWHLWPFRPGSAGTPPPRRRGVVMRGSGEFVEVGKILAVGRNY
                     AEHVREMGAPVAELPVIFFKPPTALTGEGELRLPAGAGEIHHEVELVVVVGLAGRAIS
                     AERALDHVAGYAVGLDLTARELQRVAKRRGEPWSLAKGFDGSAPVSAVAPRDEIGDGS
                     GLDIELSVNGERRQHGNTSQMLHPVPRLVAICSRWVTLEPGDLIFTGTPAGVGPLVRG
                     DLVEARIAGVGRLDLRIA"
     gene            4655..5188
                     /locus_tag="VD788_16380"
     CDS             4655..5188
                     /locus_tag="VD788_16380"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXV77889.1"
                     /translation="MTKVGTRRSALGKLVLVLALVAPAPAHAGQTAPEFAFEDINPNS
                     ATFGDKLALSARYAERGLVLNFLASWCSYCFKELPELEQLNTAGVAPVLGVAADEYDG
                     PGRLREMLGQLKLSFPVLLVPEEQIASMGESYGHRILPATYLIDKTGAVRSVFEGAVT
                     GRDLTREIQRTLRPARD"
     gene            complement(5189..5842)
                     /locus_tag="VD788_16385"
     CDS             complement(5189..5842)
                     /locus_tag="VD788_16385"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXV77890.1"
                     /translation="MKLCARLWLAVCLAVLTCGCATTFQGDHTTKRDKTTKGAAIGAA
                     SGAVLGAVLGEGELDEVLAGAAIGAGVGAGVGAYMDHQEERLARIPGTTVERVGDNLL
                     LVRFDSDVLFKVDSSTLETSARSALDEAADVFLEYRKTAIVTQGHTDSTGSEQYNLSL
                     SERRADAVAGYLVGRGVDPTRITTIGYGESQPTADNVSDWGRQQNRRVDLLLKAKVK"
     gene            complement(5927..6928)
                     /locus_tag="VD788_16390"
     CDS             complement(5927..6928)
                     /locus_tag="VD788_16390"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXV77891.1"
                     /translation="MTGQPDGGPPGLLDRLVEFLASSMNALGLNGTRLRWKWNRQRSR
                     WAESGERATNLWRSTKSKHKMCPSCRALVPRGCSRCDECGRELAGVRAPGIGRLVANL
                     LPGATAATSLILLTNGFWFLMTMMAQMKLAGGGGFGLLSGFSGELLYRFGSGWSGATL
                     NGEWWRVVTPIFLHGGILHFFFNSYVLLQIGPLVEDEYGTERFWVIYLLCGIAGASAS
                     QFLRPVNTVGASGALLGMMGLLLVHGWRRGGPTGQALKGAMIRWGVYIAIFGLLLPGI
                     DNINHLGGFVSGAALGFVAGWGPSRGRGRRRVWNMAAGAGVLLVLFSFYMVASTAVS"
BASE COUNT         1073 a         2513 c         2363 g          995 t
ORIGIN      
        1 tgctggcgcg cgatgcgttc ggcgaacgcg tgccggtcga ggtgggggcg gagctgacgc
       61 atgacgggat agcagcgatc tatctcggcc gcgcttcgtg ccagtcggat cgagctcatg
      121 atctcaccac agacgatgga aacggggatt atacgagaga gtctcttccc gcgagcgaaa
      181 ccgatcacca ctcggccgat ggccgaggcg gcccgccggg tcgatcttca ctcggatgga
      241 ccgggttcgg ccagcgctcc gctggcgccg gtcgacggca ggcctgacgc ggctcgcggc
      301 gtggtccgcg ctgctcgtcg tgctccatcg accgtggcac gagctgggcg ggacgatcgg
      361 cgagcggttg cgctggctcg aacggctggt gctgtgcttc ggtctgctcg ccggctgggc
      421 cgtcggcctg ttcgtacgcg attctgcatg ccatcggcga gggcgtccac atgcggcgtg
      481 gcttcgtttc gtctggaccc ccgcggccgg gatggccgcc ctggtgatgc tggtcgcctc
      541 accgctcgac tggcgcgtca cgctactgac cttggtcgcg atcgcggcct attcggccgg
      601 actcgacgcc gcgatcgccg gttggccgct cgcccgtggc cgggcgtacc gcttcgccga
      661 acggatcgaa tccggagacg atgcgtacga tcttgcgcgt cgcgccggac accggcgtct
      721 ctcccagccg ctcgatcgcg acccagcgca gcccgtcggc ggcggccacg cgcccggctc
      781 gccgggcgcc gcgatggacg agtagccgca ggcggcgctt catgatgccg tgggtcagct
      841 cggtggttcg cgcgccgacc gcgagtgtga gtccgtagcg ctcgcgcatc aagcgacgga
      901 tcgtctcccc gctggtgaca cccgcgcgga cgatgcgggt cggcagatcc cagttcccgc
      961 gcaacggcgt gtccgcggtc cggccggcga gcaacagctt gccgcggtgt tcgagccaga
     1021 gcaccgccgc gtcgtcgtcg agcgcggccg cccgccggga ggcggacggg tagcccgagg
     1081 gattgcccgc gctcgccgcg cggcaccggc ggcgcagtgg acagtccgtg cagtcggggt
     1141 tcgcgggcgt acagaccaac gcgccgagct ccatcagcgc ctggttgacg tcaccggggc
     1201 gcgctccacg caccagttcg ccggcagccg accagagccg ccggcgctcc tccgccggtc
     1261 cgcagaccgc tccgtcgatg gccagcagcc gcgccagcac ccgccggacg ttgccgtcga
     1321 gcaccgcggc aggcttgccg taggcgatgc tggcgatcgc cccggccgtg taccggccga
     1381 tgccgggcag ctcgatcagc accgcggggt cgtcgggcaa cctaccaccg tgccgctcga
     1441 cgatcgcccg cgcccccgcg cgcagcatgc gggcacgccg gtagtagccc aacccggtcc
     1501 aggcgctgag cacctcgtcc tcggtcgcct cggccaacgc gaacggatcg ggaaagcgcc
     1561 ggatgaagcg ccggtagtac gggatgaccg tcgccgcccg ggtctgctgc agcatcacct
     1621 ccgagaccca gatggcatag gggtcgctgg tgcgccgcca gggcaggtcg cgccggtggt
     1681 cgtcgtacca cttcaacaac gcgcggcgca cgacggacag gctcctagtc cggccgaatg
     1741 cgttccagac gcaagcgctc gaccggccga ccggcgacga tgtgttcgcg gaacagctcg
     1801 tcgacatcgt cgagggtgac ccgcccgtac cacaccgact ccgggtagac gacgatcgtg
     1861 acaccggtcg cacactggcc gagacagccg gcggagttga cgcgcacccg gcccttcagc
     1921 ccggcgtcga acgcgagctt tttcaaccgg gaacggatct cctctccgcc cttggcggca
     1981 cagcagcctt tcggattgtc cggatcgcgc cggttctcgc agacgaagac gtgtcgttcg
     2041 tacgacggca tgcgggcatt ctagcaagcg cgtcggtgcg cggccgacgt gttaggatgc
     2101 gcgcttctcc ccgcgtggag gtggacgatg ggacaatggc gcctgctcga acggcgagtc
     2161 gtccagcgct gtcgtgtctt cgacgtgtcg agcgcgacct tcgagtcggt cgagagcaac
     2221 cgcccgcatc agttcttcgt gctgaacgcg ccggactggg tcaacgtgat cccgatcaca
     2281 gacggtgggc gcgtgctgat ggtgcgtcaa taccgcttcg gcatcgccgc cgatacgctc
     2341 gagatcccgg gcgggatgtg cgatgccgga gaatccccgc tgacggccgc ccggcgcgag
     2401 ctccgcgagg agaccggcta ccgcgcgaaa cgactgaccg gcgtcggctg ggtgcacccc
     2461 aacccgccga tccaggacaa ccgttgtcac accttcgtcg cccgcgggct gtcgcaggag
     2521 gggccgccgt ccgccaccga cgacgagcgg atcgagctga tcgacgtcgc cctcgacgaa
     2581 ctgccgcggc tgctcgtcga cggcacgatc acccacgcgc tggtgctggc cgccttccag
     2641 ctgttcgacg ggtaccgccg cggtttcagt cggcaggacg gtgcctgagc atgcgggcca
     2701 gcatccggtc gatcagcgcg ggaaacaggc gcgagccgat cgccagcagc ttggcctcgg
     2761 tcgacagcac cacctcgcgc cggtccgatg ccgccatccg cacgatcgcc tcggcgaccg
     2821 actcggcgct gtgccgccgc ggtcggacgc gtcgctgacc gggtcccgag cgcagcgcgc
     2881 ggtcgtgcag ctcgctgtcg gtcgaggacg gacagaccaa cccgacgctg accccgctgc
     2941 cgtacagctc ggcgcgcagg gcgtcggcca ggccgtgcag cgcgaacttg ctggccgagt
     3001 aggccgagta gtacgggatg ccgcgcttgc cgacgatcga cgagatcagc aggatgcgac
     3061 cggtgccacg ctcgaccatc ggccggacaa acggccgcac cgtgcggacc acgccgaaga
     3121 cgttgaccgc gaacacgcgc tcgagcgcct cgtcgtgcgt gttctcgaag gtcgcgtcga
     3181 gcgagatgcc cgcgttggcc acgatcacgt cgggcaagcc gatctcggtc gccacccgcg
     3241 ccgcgagccg gtccatcgcc cgggcgtcgc tgacgtccgc gccgatcggg accaggcgcg
     3301 attcaccgcc cagctcgtcc gacagctcgc gcagccgctc gatcgcgcgc gcgacgccga
     3361 tcacccgcgc gccgcgctcg acgaagcggc gggtggcggc ccgcccgatg ccggccgagg
     3421 cgccggtgac gaggaccaag ctgccggcga tcgcgcgtgc catcagtgtc ggctcgccat
     3481 tcggctcacg cgatccgcag gtcgagtcga ccgacgccgg cgatccgcgc ctcgaccagg
     3541 tcgccccgga cgagcggccc gacaccggcc ggcgtcccgg tgaagatcag atcgcccggt
     3601 tcgagcgtga cccagcgcga acagatcgcg accaaccggg gcacaggatg gagcatctgc
     3661 gaagtgtttc cgtgctggcg tcgctcgccg ttgaccgaca gctcgatatc gaggcccgag
     3721 ccgtcgccga tctcgtcgcg cggcgcgacc gccgatacgg gagcggatcc gtcgaagccc
     3781 ttggccaggc tccacggctc gccccgtcgc ttcgccaccc gctgcagctc gcgcgccgtc
     3841 aggtcgagtc cgaccgcgta acccgccacg tgatcgagcg ctcgttcggc ggagatcgcc
     3901 cgtccggcga ggccgacgac gacgaccagc tcgacctcgt ggtggatctc gccggcaccg
     3961 gccggcaagc gcagctcgcc ttcgccggtc agcgccgtcg gcggtttgaa gaagatcacc
     4021 ggtagttcgg ccaccggcgc tcccatctca cgaacgtgtt cggcgtaatt gcgaccgacg
     4081 gccaggatct tgccgacctc gacgaactcg ccgctgcccc gcatgacgac gccgcggcgc
     4141 cggggtgggg gagtgcccgc cgagccgggg cggaacggcc acaggtgcca ggtgcccgat
     4201 cccgcggcga ccagcgtccc gcgttcgtcg tggatctcgc cgctggcgaa cgcgacgctg
     4261 cgacctcgcc gcagaacgcg gccggtcgcg accagccggc cgccgcgggc acccccgatg
     4321 aagtgcgcgg tcagcgacgt cgtggcgcac caccactcct gcgggatgtc gctgatcacg
     4381 gctgcgccga gcgcactgtc gagcagggcg gtgacgacgc cgccgtgcgc cacgccccgc
     4441 cggttgagat ggtgcggctg cagttcgagc gttgcctcgg cctgcccttt gccgcgccga
     4501 acgatctcgg tccccaggta agcgttgaac ggcggcactc ccatccgcac ctcctcggcc
     4561 ggcgtcccga ccaccgcgtc gattctaata ccgcgccggc gtgcgacggt cgtcgacccg
     4621 tcgtatcctt acccggatcg atgtgggagt gacgatgacc aaggtcggaa cgcgacggtc
     4681 ggcgctgggg aaactcgtgc tcgtgctcgc gctggtcgcg ccggcgccgg ctcacgcggg
     4741 acagaccgcg cccgagttcg cattcgagga catcaatccg aacagcgcca cgttcggtga
     4801 caagctcgcg ctgagcgcgc ggtacgccga acgcgggctg gtgctgaatt tcctcgcctc
     4861 gtggtgcagc tactgcttca aggagctgcc ggagctcgag cagctgaaca cggccggcgt
     4921 ggcgccggtg ctcggcgtgg cggcagacga atacgacggg ccgggccggc tgcgcgagat
     4981 gctcggccaa ctgaagctga gcttccccgt gctgctggtg cccgaggagc agatcgcctc
     5041 gatgggcgag agctatggcc accgcatcct gcccgcgacc tacctgatcg acaagaccgg
     5101 cgcggtccgt tcggtcttcg agggcgcggt gacggggcgc gacctgacac gggagatcca
     5161 gcgaacactg cgccccgcgc gagactgact acttcacctt ggccttcagc agcaggtcga
     5221 cccgccggtt ctgctggcgc ccccagtcgg aaacgttgtc ggccgtcggc tggctctcgc
     5281 cgtaaccgat cgtggtgatg cgcgtcgggt cgacgccgcg cccgacgagg taacccgcga
     5341 ccgcgtcggc gcggcgctcg gacaacgaca ggttgtactg ctcgctgccg gtcgagtcgg
     5401 tgtggccctg ggtcacgatc gccgtcttgc ggtactccag gaagacgtcg gccgcctcat
     5461 cgagcgcgct gcgcgcgctg gtctcgagcg tgctcgaatc gactttgaac aagacgtccg
     5521 aatcgaaacg gacgagcagc agattatcgc cgacgcgctc gaccgtggtg cccggaatcc
     5581 gcgccagccg ctcttcttga tgatccatgt aggcgccgac gccggcgccg acgccggcgc
     5641 cgatcgccgc accggcgaga acttcgtcca actcaccctc gccgagcacc gcgccgagca
     5701 cggcacccga ggccgcgccg atggccgcac ccttggtcgt cttgtcccgc ttggtcgtat
     5761 ggtcgccctg gaacgtcgtg gcgcagccgc acgtcagcac cgcgagacag accgcaagcc
     5821 acaatcgcgc gcacagcttc atcgcgtgcc ctcctgtttc ggccggccga cccgtcggcc
     5881 gggcgccggg ttcgcacgat ctaacatagc cccccgaccg cgacggtcaa gacaccgccg
     5941 tcgaggcgac catgtagaac gagaacagca cgagcaacac gccggcgccg gccgccatgt
     6001 tccacacccg gcgccggccg cggccgcggg acggacccca gccggcgacg aagcccagcg
     6061 ccgcgccgga aacgaatccg ccgaggtggt tgatgttgtc gatcccgggg agcaacaggc
     6121 cgaagatcgc gatgtagacg ccccagcgga tcatcgcgcc cttcagcgcc tgcccggtcg
     6181 gaccaccgcg acgccagccg tgcacgagca acagccccat catgccgagc agcgctcccg
     6241 aggcaccgac ggtattgacc ggacggagga actgactggc cgacgcgccc gcgatcccgc
     6301 acagcaggta gatcacccag aagcgctccg tcccgtactc gtcctcgacc aacggaccga
     6361 tctgcaacag cacgtacgag ttgaagaaga aatggaggat cccgccgtga aggaagatcg
     6421 gcgtgacgac ccgccaccac tcgccgttga gggtcgcccc gctccaaccg gagccgaagc
     6481 ggtaaagcag ctcgccgctg aagccgctga gcaagccgaa gccgcctccg ccggcgagct
     6541 tcatctgcgc catcatcgtc atcaggaacc agaagccgtt ggtcaacaag atcagcgacg
     6601 tcgccgccgt cgcgccgggc agcaggttcg cgaccaaccg gccgatgccc ggtgcgcgca
     6661 cgcccgccag ctcacgcccg cactcgtcac agcgcgaaca gccgcggggc acgagggcgc
     6721 ggcacgacgg gcacatcttg tgcttgctct tggtcgagcg ccacaggttg gtcgcgcgtt
     6781 cccccgactc ggcccaccgc gagcgctgcc gattccattt ccagcgcagt cgcgtcccgt
     6841 tcagaccgag cgcgttcatc gacgaggcga ggaactcgac caggcggtcg agcaggcccg
     6901 gtgggccccc gtccggctgg ccggtcattc agtggccctc gaag
//
LOCUS       DASPGN010000567         4633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_341826, whole genome shotgun sequence.
ACCESSION   DASPGN010000567 DASPGN010000000
VERSION     DASPGN010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341826"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..2204)
                     /locus_tag="VD788_16395"
     CDS             complement(171..2204)
                     /locus_tag="VD788_16395"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXV77892.1"
                     /translation="MRPIRPTTAIGPAVAAVAAAIAASVTPAAPAPKVFVLGIDGFDP
                     ELMQRFIDDGELPNFARLAAEGDYTPLQTTMPPQSPVAWSSFITGMDPGGHGIFDFVH
                     RDPETILPYLSMARAEPPSRSVALGRFVLPLASGSVVNLRRGVAFWEILERNGVPTTV
                     FRMPANFPPVEAGGRSLSGMGTPDIKGTSGTFSFYTDREVPNAKEITGGEIYYVTVEN
                     NRVRSTLHGPENSFRRVPRKQRATDGSLRYTHPDMTVDFTVDLDPELPVAMIEVGDRR
                     LVLEQGEWSPWVPLEFEAVPWLISVSAIGRFYLKQVRPDFELYVTPLQIDPADPALPI
                     STPASWSSELHRRLGYFYTQELPEDTKALSGGVFTGEEFWDQFQFVYQEQSRALDYLL
                     SEFDEGFMFFYFSSVDQGSHMLWRYADPDHPAYEEHTTLLDGIRTTYRQLDDALGRVL
                     RVIDDETTLIVMSDHGFCPFYRGVNLNSWLVENGYVTLRDPAAQGRSSGIPFANVDWS
                     RTRAYALGLNGLYVNLAGREERGIVQPGDEYERLLDELDRRLLALTDPANGQPVVTLV
                     TRPGREFHGPQASSGPDLLVGYNRGYRSSWTSPLGEFPRGVFVDNDEAWSGDHLVDYR
                     HVPGVLLSNRQISCADPALYDLTVAILDEYGIDKTPEMIGRDCLGSTGRERRR"
     gene            complement(2201..4408)
                     /locus_tag="VD788_16400"
     CDS             complement(2201..4408)
                     /locus_tag="VD788_16400"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXV77893.1"
                     /translation="MTRSAERQLCLALTAVTLLALMLDPPPVQAYIGPGAGFAFVGSF
                     FVIIATFFLAFLKLITAPLRAVVRWLRSRKALRRSRVRRVVIVGLDGQDPELTDRFLG
                     AGLLPNFARLRQRGGYVRLDTSFPAESPVAWSSFQTGCNPGKHKIFDFLVPNRKSHLP
                     ELCSAKVRGASRTLKLGRYRFPLGKPSIDLGRKSRPFWKVLGDHGIFSSVIRVPITFP
                     PEKFHGVMLSAMCVPDLKGSQGTFSYYSDEPRGEGGFTGGMRIPVEVRDGVVRSHISG
                     PDNSMVDGGGELRIPFEVQLDRDGTGARLIIGRKSYELRPRQYTPWIALEFRAALWFK
                     VRGIVRFYLLDTAPHFRLYMTPINIDPDKPALPISHPFTYSVYLSKTQGRFSTLGLAE
                     DTWALNERVLDEQAFVEQAYLIHDERERMLLDAIEKTKRGAVVCVFDLTDRMQHMFWR
                     YLDAEHPANAGKDVDRHRDTLEEMYVRMDDLVGRVAAAVDDETVLMVMSDHGFKPFKR
                     GVNLNTWLWERGYLALRGDSPTGAQWYQDVDWTKTRAYAVGLGGIYLNLRGREAKGIV
                     EPGEEQARIKREIAAGLRELRDEQVGAPRAVAEVYDTSEIYSGPYVREAPDLFVGFAV
                     GYRASWDCATGEVAPTTIRDNTKSWSGDHCMNPPDVPGVFFCSHALDVPRVNIMDIGP
                     TVLDLFGVEVPAYCDGRPVMTGRPRTAERPSGDRADAPAPASAGVRFDGESAR"
     gene            complement(4412..>4633)
                     /locus_tag="VD788_16405"
     CDS             complement(4412..>4633)
                     /locus_tag="VD788_16405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77894.1"
                     /translation="EKMTRLYLDHLRKVARQLDGRRNFERLDVDYRATIDDPRGQAER
                     VARFLELPLDTARMVAAVDPTLYRNRDRS"
BASE COUNT          770 a         1542 c         1581 g          740 t
ORIGIN      
        1 ctgcagcttc ttgcggatct cgtcttccga gtccgcttcc tcgagctggg cgatctcctt
       61 ctcgagcgcg tgggtgatga actcttcggc agaagaatag ccggcgatgt cggcgtactt
      121 cttgatcttc ttcagcagct cacggtcgag cttgactcgc gaaccgaaca tcaacgcctc
      181 ctttcgcgtc ccgtcgaccc caggcagtcc cggccgatca tctccggcgt cttgtcgatc
      241 ccgtactcgt cgaggatcgc caccgtcagg tcgtacagcg cgggatcggc acagcttatc
      301 tggcggttgc tcaacagcac gcccggtacg tgccggtagt cgaccaggtg gtcaccgctc
      361 cacgcctcgt cgttgtcgac gaagacgccg cgcgggaact cgcccagcgg gctggtccac
      421 gacgagcggt aaccgcgatt gtagccgacc agcaggtcgg gtcccgagct ggcctgcggt
      481 ccatggaact cgcgaccggg ccgggtgacg agcgtgacga ccggctgacc gttggcggga
      541 tcggtcagcg cgagcaagcg gcggtcgagc tcgtcgagca gccgctcgta ctcgtcgccc
      601 ggctggacga tgccgcgctc ctcgcgtccg gcgaggttga cgtacaaccc gttcaacccc
      661 agcgcgtagg cccgcgtcct cgaccagtcg acgttggcga acgggatacc gctcgatcgt
      721 ccttgcgccg ccgggtcgcg cagcgtgacg tagccgttct cgaccagcca gctgttgaga
      781 ttgacgccac ggtaaaacgg gcaaaagccg tgatccgaca tcacgatcag cgtcgtctcg
      841 tcgtcgatca cgcgcagcac gcggccgagg gcatcgtcca gctgacggta ggtcgtgcgg
      901 atcccgtcga gcaacgtcgt gtgctcttcg taagccgggt ggtccgggtc ggcgtagcgc
      961 cacagcatgt ggctgccctg atcgacgctg gagaagtaga agaacatgaa tccctcgtcg
     1021 aactcggaga gcaggtagtc gagtgcgcgg ctctgctcct ggtagacgaa ctggaactgg
     1081 tcccagaact cctcgccggt gaagaccccg cccgacagcg ccttggtatc ctccggcagc
     1141 tcctgcgtgt agaagtaacc gagccgccga tggagctcgc tggaccacga cgccggggtc
     1201 gagatcggca gcgccgggtc ggccggatcg atctgcaacg gcgtgacgta cagctcgaag
     1261 tcgggtcgca cctgcttcag gtagaagcgg ccgatcgcgc tgaccgagat gagccacggc
     1321 accgcttcga actcgagcgg gacccacggg ctccactcgc cctgctcgag caccaaccgg
     1381 cgatcgccga cttcgatcat cgccaccggc agctcggggt cgaggtccac ggtgaaatcg
     1441 accgtcatgt cggggtgcgt gtagcggagc gagccgtccg tcgcccgctg tttgcgcggc
     1501 acgcggcgga aggagttctc cggaccgtgg agcgtcgaac ggacccggtt gttctccacg
     1561 gtcacgtagt agatctcgcc gccggtgatc tccttggcgt tcggcacctc gcggtcggtg
     1621 tagaacgaga aggtgccgga ggtgcccttg atgtcgggcg tgcccatgcc ggacaacgag
     1681 cgtccaccgg cctcgaccgg cgggaagttg gccggcatgc ggaagaccgt cgtcggcacg
     1741 ccgtttcgct cgaggatctc ccagaacgcg accccgcgcc gcagattcac gaccgagccc
     1801 gaggcgagcg gcagcacgaa ccgaccgagc gccacgctgc ggctcggcgg ctcggcacgg
     1861 gccatcgaca ggtagggcag gatcgtctcg gggtcgcgat ggacgaaatc gaagataccg
     1921 tgcccccccg ggtccatgcc ggtgatgaag ctcgaccaag ccaccggact ctgtggcggc
     1981 atcgtcgtct ggagcggcgt gtaatcaccc tcggccgcca gccgcgcgaa gttcggcagc
     2041 tcgccgtcgt cgatgaagcg ctgcatcagc tcggggtcga agccgtcgat gccgagcacg
     2101 aagaccttgg gcgccggcgc cgccggcgtc acgctcgccg cgatcgccgc ggcaacggcg
     2161 gcgacggcgg gaccgatcgc ggtcgtcggg cggatcggcc tcatcgtgcc gactcgccgt
     2221 cgaagcggac gccggccgac gcgggggccg gggcatcggc gcgatcgccg gacggccgtt
     2281 ccgccgtccg cggccgcccg gtcatcaccg gtcggccgtc gcaataggcc ggcacctcga
     2341 cgccgaacag gtcgagcacg gtcggtccga tgtccatgat gttgacccgc ggcacgtcga
     2401 gcgcgtggct gcagaagaag acgccgggca cgtcgggcgg attcatgcag tgatcgccgc
     2461 tccagctctt ggtattgtcc cgaatcgtcg tcggcgccac ctcgccggtc gcgcagtccc
     2521 acgaggcgcg gtagccgacg gcgaaaccga cgaacaggtc gggtgcctcg cgcacgtaag
     2581 ggccgctgta gatctcgctc gtatcgtaaa cctcggccac cgcgcgtggg gcaccgacct
     2641 gctcgtcgcg caactcgcgc aagccggcgg cgatctcgcg cttgatccgc gcctgttcct
     2701 cgccgggctc gacgataccc ttggcctccc gtccgcgcag gttgaggtag attccgccga
     2761 gaccgacggc atacgctcgg gtcttcgtcc aatcgacgtc ctggtaccac tgggcgccgg
     2821 tcggcgagtc gccgcggagc gccaggtagc cgcgctccca caaccaggtg ttcagattga
     2881 cgccgcgctt gaacggcttg aagccgtggt cggacatcac catcagcacc gtctcgtcgt
     2941 cgaccgcggc ggcgacgcga ccgaccagat cgtccattcg gacgtacatc tcctcgagcg
     3001 tgtcgcggtg ccggtcgacg tccttgccgg cgttggccgg atgttcggcg tcgaggtagc
     3061 gccagaacat gtgctgcatg cggtcggtca ggtcgaaaac gcagacgacc gcaccgcgct
     3121 tggtcttctc gatcgcgtcg agcaacatcc gttcgcgctc gtcgtgaatc aggtaggcct
     3181 gttcgacgaa ggcctgctcg tcgagcacgc gctcgttgag cgcccaggtg tcctcggcca
     3241 gaccgagggt cgagaagcgt ccctgcgtct tcgacaggta gaccgaatag gtgaacggat
     3301 gcgagatcgg cagcgccggc ttgtccgggt cgatgttgat cggcgtcatg taaaggcgga
     3361 agtgcggcgc ggtgtcgagc aggtagaagc gcacgatgcc gcggaccttg aaccacaacg
     3421 cggcgcggaa ctcgagcgcg atccacggcg tgtactggcg cggcctcagc tcgtacgatt
     3481 tccgcccgat gatcagccgc gcgcccgtgc cgtcccggtc gagttgcacc tcgaacggga
     3541 tgcgcagctc gccgccgccg tcgaccatcg agttgtcggg ccccgagatg tggctgcgga
     3601 ccacgccgtc gcgcacctcg accggaatgc gcatgccgcc ggtgaacccg ccctcgccgc
     3661 gcggctcgtc actgtagtag gaaaaggtgc cctggctgcc cttcaggtcg ggaacgcaca
     3721 tcgccgacag catcacgccg tggaacttct cgggcgggaa ggtgatcggc acgcgaatca
     3781 ccgagctgaa gatcccgtgg tcgccgagca ccttccagaa cggccggctc ttccgaccga
     3841 ggtcgatcga cggcttgccg agcgggaatc ggtagcggcc gagcttcaac gtgcgcgagg
     3901 cgccccgcac cttggccgag cacagctcgg gcaagtgcga cttgcgattg ggcacgagga
     3961 aatcgaagat cttgtgcttg ccggggttgc agccggtctg gaagctcgac caggcgaccg
     4021 gcgattcggc ggggaacgag gtatcgagcc ggacgtagcc gccgcgctgg cgcaggcgcg
     4081 cgaagttggg tagcaacccc gcgccgagaa agcgatcggt cagctccggg tcctggccgt
     4141 cgagaccgac gatcaccacg cggcggacgc ggctcctgcg cagcgccttg cgcgaacgca
     4201 gccagcgcac gacggcccgc agcggtgccg tgatcagctt gaggaacgcc aggaagaagg
     4261 tggcgatgat gacgaagaac gagccgacga aggcgaagcc ggcgcccggg ccgatatagg
     4321 cctgcaccgg cggcggatcg agcatcagcg ccagcagcgt gacggcggtc agtgccaggc
     4381 agagctgccg ttcagccgat cgcgtcacga ctcagctccg gtcgcggttg cgatagaggg
     4441 tcggatcgac ggcggccacc atccgagcgg tgtcgagcgg cagctcgaga aaacgtgcga
     4501 cgcgctcggc ctgcccgcgc gggtcgtcga tcgtcgcgcg atagtcgacg tcgagccgct
     4561 cgaagttgcg cctgccgtcg agctggcgcg cgaccttgcg caggtggtcg agatacagcc
     4621 gcgtcatctt ctc
//
LOCUS       DASPGN010000568         2742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_574976, whole genome shotgun sequence.
ACCESSION   DASPGN010000568 DASPGN010000000
VERSION     DASPGN010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574976"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VD788_16410"
     CDS             <1..1351
                     /locus_tag="VD788_16410"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXV77895.1"
                     /translation="ADGLVRIVRWAKDGLRVLRAPLDESARIGPAGRRLRETVVRAAA
                     AGDLGGDGRKRVVVASKDGRVYVFDGDGRRVPGFPVSIDPDLAEPTGPEQVIEHGILS
                     RPVLVDLDGKPGLEILVSALDGHLYAWRHDGKPLDGFPVAVHPAGERASHRAKIVSTP
                     AVGDIDGDGALEIVVGSNHVHDGRAGVYAVRARGNAQPGGAFLAGWRPFPVDGVRPDL
                     LPTVARGLQMDPVLVDADDDGDDEVFVYAVTGNRIKLIDQPADGPPRVSAEFSLSPGT
                     RSVFRDTAFLGGTGSPALIDADADGTPELYAPLLPMRMITLRFKPGVPIDVPTVLGGW
                     PLVASADHSAPVEMLPDYPRRMEDLMLLVRPAFADVDGDGKPEVLVGSGGYLLHAFAA
                     GGGEAPGFPKFTGGWLFSDAAVGDLDGDGVSELVSVTREGYLFAWQLAAAARPASDR"
     gene            complement(1278..>2742)
                     /locus_tag="VD788_16415"
     CDS             complement(1278..>2742)
                     /locus_tag="VD788_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77896.1"
                     /translation="VEAAEAPPFFDPNAVVVNLTEDDLNLALEDLWSALGARFSGTRE
                     QVSSTARGLHYDGRLSTPVIDLMPDGRASVRLALEEATVRLDSVEHTLLGRRVRCEDI
                     GADLQPDRVLDLELRLDFRLEREDLRIVPTAVDVADPDGAFRLIKPSRCRRNLIPTFM
                     LWWIAKPFLQKQFREIDQKLLASAERGAAQLEDDDGTILRKRVDVEGHELVIYPRRLT
                     TDGDALWIVAAGASDESHPGTTSAEPPRALGRSSARSFLAVSEPTLNAILDRAYLEWS
                     RPTDRPSGSLRKLFQSDELLTLVPGLREVENRERLHWGIRFTSPPRVRLETLRDIDAR
                     NGDAFSLYAWSPIADQPVITLISSGIEVEIARDDAPGEPLGVLSIDSGVVSAIPYANR
                     LGGLSLQVVRNEWEVSSRGIRFEQRLLAATLQELIFAELFETRYEPLARGALTVGPIR
                     FEPTGFAVAEGYLIIDLKRVAPPPQAATRTDSPRASR"
BASE COUNT          379 a          963 c          997 g          403 t
ORIGIN      
        1 ggccgacggg ctcgtgcgca tcgtgcgctg ggcgaaggac gggctgcgcg tgctccgcgc
       61 gccgctcgac gagagcgcgc ggatcggccc ggccgggcgc cgcctgcgcg agacggtcgt
      121 gcgcgcggcg gcggccggcg acctcggcgg cgacggccgg aagcgcgtcg tcgtcgcctc
      181 gaaggacggg agagtctacg tcttcgacgg cgacggccgc cgcgtcccgg gcttcccggt
      241 gtcgatcgat ccggacctgg ccgagccgac cggtcccgag caggtgatcg agcacggcat
      301 cctgtcgcgt ccggtgctgg tcgacctcga cggcaagccg ggcctcgaga tcctcgtctc
      361 cgcgctcgac gggcatctct acgcctggcg ccacgacggc aagccgctcg acgggtttcc
      421 ggtcgcggtc caccccgccg gtgaacgagc gtcccaccgc gccaagatcg tctcgacccc
      481 cgcggtcggc gacatcgacg gcgacggcgc gctcgagatc gtcgtcggct cgaatcacgt
      541 gcacgacggc cgggccggcg tctacgccgt gcgcgcgcgc ggcaacgccc agccgggcgg
      601 ggcctttctc gccggctggc ggccgtttcc ggtcgacggc gtgcgacccg acctgctgcc
      661 gaccgtcgcc cgcggcctcc agatggaccc ggtgctcgtc gacgccgacg acgacggcga
      721 cgacgaggtc ttcgtctacg ccgtgacggg caaccgtatc aagctgatcg accagccggc
      781 cgacggcccg ccgcgggtat cggccgagtt ctcgctgtcg cccgggacgc ggtcggtctt
      841 ccgcgacacg gccttcctcg gcggcaccgg ctcacccgca ctgatcgacg ccgacgcgga
      901 cggcacgccc gagctgtacg cgccgctgtt gccgatgcgc atgatcacgc tccgcttcaa
      961 gccgggggtg ccgatcgacg tcccgacggt tctcggcggt tggccgctcg tcgcctcggc
     1021 ggaccactcg gcgcccgtcg agatgctgcc ggactacccg cgacgcatgg aagacctgat
     1081 gctgttggtg cggccggcgt tcgccgacgt cgacggcgac ggcaagccgg aggtgctggt
     1141 cggcagcggc ggatacctgc tgcacgcgtt cgcggcgggc ggaggcgagg cgccgggctt
     1201 ccccaagttc accggtggct ggctgttctc cgatgcggcg gtcggcgatc tcgacggcga
     1261 cggcgtgtcg gagctggtca gcgtgacgcg cgagggctat ctgttcgcgt ggcagcttgc
     1321 ggcggcggcg cgacccgctt cagatcgatg atcaggtagc cctcggcgac cgcgaacccg
     1381 gtcggctcga accggatcgg gccgaccgtc aacgcaccgc gggcgagcgg ttcgtagcgc
     1441 gtctcgaaca gctcggcgaa gatcagctcc tggagcgtgg ccgcgagcag gcgctgttcg
     1501 aagcggatgc cccgcgacga aacctcccac tcgttccgca cgacctgcag cgagagaccg
     1561 cccagccggt tggcgtacgg gatcgcgctg accacgcccg agtcgatcga caagacgccg
     1621 agcggctcgc ccggtgcgtc gtcgcgggcg atctcgacct cgatgccgga cgagatcagc
     1681 gtgatcaccg gctgatcggc gatcggcgac caggcgtaca acgagaacgc gtcgccgttc
     1741 cgggcatcga tgtcgcgcag cgtttccaac cggacccgcg gtggcgaggt gaagcggatc
     1801 ccccagtgca gccgctcgcg gttctccacc tcgcgcaggc ccggcacgag cgtcagtagc
     1861 tcgtcgctct gaaacagctt gcgcaagctg ccgctcggcc ggtcggtcgg tcgcgaccac
     1921 tcgaggtagg cacggtcgag gatcgcgttg agcgtgggct cggacacggc gagaaacgac
     1981 cgagcggagg aacggccgag cgcacggggc ggctcggcgg atgtcgtccc ggggtgcgac
     2041 tcgtcgctcg cgccggcggc gacgatccac agggcgtcgc cgtccgtcgt cagtcgtcgc
     2101 gggtagatca cgagctcgtg gccctcgacg tcgacgcgct tgcgcaggat cgtgccgtcg
     2161 tcgtcttcga gctgcgcggc accgcgttcg gcgctggcga gcagcttttg gtcgatctcg
     2221 cggaactgct tttgcaggaa cggcttggcg atccaccaca gcatgaaggt cgggatcagg
     2281 ttgcggcggc agcgggaggg cttgatcagt cggaaggcgc cgtcggggtc ggcgacgtcg
     2341 accgcggtcg gcacgatccg caggtcttcc cgctcgaggc gaaagtcgag ccgcagctcg
     2401 agatcgagga cccggtcggg ctggagatcg gcgccgatgt cctcgcagcg gacgcgccgg
     2461 ccgagcaacg tgtgctccac actgtcgaga cgcacggtcg cttcctcgag tgccagtcgc
     2521 accgacgctc ggccgtccgg catcagatcg atcacgggcg tcgacagccg cccgtcgtag
     2581 tgcaggcccc gcgccgtcga cgagacctgc tcccgcgtcc cgctgaatct cgcgcccagc
     2641 gcgctccaca ggtcctcgag cgcgaggttg agatcgtcct cggtcaggtt gacgacgacg
     2701 gcgttggggt cgaagaacgg cggcgcttcg gcggcctcga cc
//
LOCUS       DASPGN010000569         2463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1277281, whole genome shotgun sequence.
ACCESSION   DASPGN010000569 DASPGN010000000
VERSION     DASPGN010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1277281"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..2120
                     /gene="argS"
                     /locus_tag="VD788_16420"
     CDS             135..2120
                     /gene="argS"
                     /locus_tag="VD788_16420"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXV77897.1"
                     /translation="MDLLTLERAFSLALRAAVRQELDLDVADVPLSYPPDAELGDIAT
                     PVCFELAKRARRPPREIAERVARALRTDDRIARVEVAGAGYVNAFLDRQSVLRRWLAE
                     DEAARDCVPGKTIVEHTNINPNKAAHIGHLRNAVLGDTLVRFLRRLGHVVEVQNYIDD
                     TGVQVADLVVGFLHLRGQGAREVREQADRGERSADPGSRFDYLAWELYAEVTRFYDAD
                     GDRLALRHTVLEEMEQGGNPTAELAAFVSQRMVQHHLATMDRIGVRYDLLPHESDILR
                     LRFWDRAFRLLRESNAVLRVDNGKNAGCWVMNLTDVEQGSGEDQKVIVRSNGTVTYVG
                     KDIAYQLWKFGLLARDFAYTRFDWRPAQEIYPLWATCSTAGETDHPPFGRATRVYNVI
                     DTRQSYLQRVVHQGLERLGYRAQAAQSIHYSYEMVALTPAAVAEIFPEHPLSAEDRTK
                     AYLEMSGRKGLGVRADDLLDRLETRAADEVRKRNPDGDPRQTARCIAVAALRYYMLRY
                     SRNRVVAFDLDAALAFEGETGPYLQYTIVRARNILAKVAERHGAEEVERTALAERAGL
                     DGLSAAERVDHWNLLTLLLRVDAVVRQSVEALELSGVAKHAYLLAQSFNSFYHKYPVA
                     QEARSDARSVRAAVVRLYHDEMVSLLGLMGIDVPDRM"
     gene            2128..>2463
                     /locus_tag="VD788_16425"
     CDS             2128..>2463
                     /locus_tag="VD788_16425"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HXV77898.1"
                     /translation="MKRLLVIIGSAVGCVTTLVAADLQTMSFDEVRAGMRGVGRTVFS
                     GTEVTSFDVEVVGKLPNIGPDQNLILARCSGGPLATTGVLSGMSGSPVWIDGKLIGAV
                     AYSWGFAKEP"
BASE COUNT          377 a          821 c          867 g          398 t
ORIGIN      
        1 agttacaccg aacgagtcgt cgcgccgccc cgtcagtgat cccgtcgcgc cggcgagttg
       61 gtcgtcgcgc gagcggtgtt ctatagtctg ccgctcggcg atccgacacg gagcgccgag
      121 cggagccgac ggcgttggat ctactcaccc tggaacgggc cttctcgctc gcgttacgcg
      181 cggcggtgcg ccaggagctg gacctcgatg tcgccgacgt gccgctgtcc tacccgccgg
      241 atgccgagct cggggatatc gccacaccgg tttgcttcga gctggccaag cgcgctcggc
      301 ggccgccacg cgagatcgcg gagcgcgtcg cgcgtgcgtt gcgaacggac gatcggatcg
      361 cacgggtcga ggtggcgggt gccggctacg tcaacgcgtt tctcgatcgg cagtcggtgc
      421 tgcgtcgctg gctcgccgag gacgaagcgg cgcgtgactg cgtcccgggc aagaccatcg
      481 tcgagcacac gaacatcaac ccgaacaagg cggcgcacat cggccatctg cgcaacgcgg
      541 tgctgggcga cacgttggtg cgctttctca gacggctggg ccacgtggtc gaggtgcaga
      601 actacatcga cgacacgggc gtccaggtcg ccgatctcgt cgtcggcttc ctgcacctgc
      661 gtggtcaggg tgcgcgcgaa gtgcgcgagc aggcggaccg cggcgagcgg tcggccgacc
      721 ccgggtcgcg cttcgactac ctggcgtggg agctctacgc cgaggtcacc cggttctacg
      781 acgccgacgg cgatcgtctg gcgctgcgcc acaccgtgct cgaggaaatg gagcagggcg
      841 gcaacccgac ggcggagctc gcggcgttcg tgtcacagcg catggtccag caccacctgg
      901 cgacgatgga tcgcatcggc gtgcgctacg acctgctgcc gcacgagagc gacatcctgc
      961 gtctgcggtt ctgggatcgt gcgttccgcc tgctgcggga gtcgaacgct gtgctccgtg
     1021 tcgacaacgg gaagaacgcg ggctgctggg tgatgaacct gaccgacgtc gagcagggct
     1081 cgggcgagga ccagaaggtg atcgtccgct cgaacgggac ggtgacctac gtcggcaagg
     1141 acatcgccta ccagctgtgg aagttcggtc tgctcgcccg ggacttcgcc tacacccgct
     1201 tcgactggcg gccggcacag gagatctacc cgttgtgggc gacctgttcg accgccggcg
     1261 aaaccgacca tccgccgttc ggccgcgcga cgcgggtcta caacgtgatc gatacgcgcc
     1321 agtcctacct gcagcgcgtc gtccatcagg ggctcgagcg cctgggctat cgcgcccagg
     1381 cggcgcagtc gatccactac tcctacgaga tggtggcgct gaccccggcg gcggtggccg
     1441 agatcttccc cgagcacccg ctgtccgccg aggatcgcac caaggcctac ctcgagatgt
     1501 ccgggcgcaa ggggctcggc gtgcgggccg acgacctgct cgatcgtctc gagacgcgtg
     1561 cggcggacga ggtgcgcaag cgcaaccccg acggcgaccc gcggcagacg gcccgctgca
     1621 tcgcggttgc cgccctgcgc tactacatgc tgcggtactc gcggaatcgg gtggtcgcgt
     1681 tcgacctcga tgcggcgctc gcgttcgaag gcgagaccgg accctatctc caatacacga
     1741 tcgtgcgcgc ccgcaacatc ctggccaagg tcgccgagcg ccacggtgcg gaagaggtcg
     1801 agcggaccgc gctggccgag cgcgccgggc tcgacggcct gtcggccgcc gagcgggtcg
     1861 atcactggaa tctgctgacg ctgctgctgc gcgtcgacgc ggtcgtgcgc cagtcggtcg
     1921 aggcgctcga gctgtccggc gtggccaagc acgcctacct gctggcgcag tcgttcaatt
     1981 ccttctacca caaatacccg gtggcccagg aggcccgcag cgacgcgcgg tccgtgcgcg
     2041 cggcggtcgt acggctgtac catgacgaaa tggtgtcgct gctcgggttg atgggaatcg
     2101 acgttcccga tcgaatgtag ccggtcgatg aaacgattgc tcgtgatcat cggatcggcc
     2161 gtcggttgcg tcacgacgct ggtcgcggcc gacctccaga cgatgtcgtt cgacgaggtg
     2221 cgcgccggga tgcggggcgt cgggcgaacg gtcttttccg ggacggaggt cacgtcgttc
     2281 gacgtcgagg tcgtcggcaa gctcccgaac atcggccccg atcagaatct gatcctcgcg
     2341 cgctgctccg gcgggccgct cgcgacgacc ggggtgctct ccggcatgag cggcagcccg
     2401 gtgtggatcg acggcaagct gatcggcgcc gtcgcctaca gctggggctt cgccaaggaa
     2461 ccg
//
LOCUS       DASPGN010000570         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1042086, whole genome shotgun sequence.
ACCESSION   DASPGN010000570 DASPGN010000000
VERSION     DASPGN010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042086"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1737)
                     /locus_tag="VD788_16430"
     CDS             complement(115..1737)
                     /locus_tag="VD788_16430"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV77899.1"
                     /translation="MNGGAGRAGSRRELAAFALLSAMLFLPGLAARDLWNPNEPLYGR
                     VVVEMYERGDWWIPTFNGRVFDEKPILYFWLALGLSTVAGGVSEWSLRLPSALAGSVG
                     VALVYRLALTHAGHRRARIAAALFATTYLVFWSARSVQMDLLLSVTTLAAVLAGTRAM
                     RERGTAVVAWSLCGASVGLGFLAKGPVGVICPLLALGAHAAVARVLPSVRGTMLATAV
                     AAVVAVPWLLVLLGRGQHEFLDELLVRQNVTRFLAPWDHERAWWYFLLHFWLDMAPWG
                     FLLLLVRLSGREAASVRLLDRWCVAWILTIVAFFSLSASKRSPYILPVAPAVAILAAG
                     TVERWLDRTLDPVRRAALWLIAAAMTVALPLLGGYAWLVLRVRYPTLAQPLTAAAVVL
                     GVGALAAAWSLARARARPALAPAALFATVVALYLLAATWALPALDPLKSSRPFCEEVR
                     SITGDAAAIRSYRGWEWRAGYIYYLGREIPRIETPAALRAYWESSEPVFLVVEDGQLD
                     EVRDALGSIEPLVKRPVGSKTAYLFTNARSRR"
     gene            complement(1734..3530)
                     /locus_tag="VD788_16435"
     CDS             complement(1734..3530)
                     /locus_tag="VD788_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV77900.1"
                     /translation="MRDFRRLLLYLRPYLGSMVLAAGLLGVGGALMAAVVSAAEPLVD
                     RVLLPSVGWTDGGGGAPTVELPRPLSGWIDRRPYLTVPAIIVAIFLVRGAVLYLGQYL
                     TVRTGALVIRDLRLALYGSVIGQSLVFFRAHTSGLILSRILGDVQRLHRIVTSQLADL
                     VRVGTMVPFLLATALYHEWRLSLVMLVALPLLGWPMLRLGRRLRRAAAASQEQMAFVA
                     DRVAESVAGIEVVQSFGMEAHEIDRFRRTLAQLVRAELRAGRAQALAPSIMEWLGAVV
                     GAGLFAAAGQAIHRGALDAGRFTVVLFSLGLLFTSIRRLNTVYAEAQRAVAAAARVFE
                     LVEQRPRIVDAAGARALPPFSASVEFRHVSFDYGDGRVLDRIDLTIARGTTLALVGPS
                     GSGKSTLARLLLRFDDPVSGAIEIDGHDLRRVTLKSLRDQIGLVTHDSMLFGDSVRDN
                     IAYGRDDVPLEAVIAAARAADAHDFIARLPRGYDTPLGERGARLSSGQRQRIAIARAL
                     VKDPPLLILDEATSALDPRSEAAVRRALRRLMRGRTCLIIAHREATVRAADRIVVLDG
                     GRIVEQGEHDGLLARGGLYARLYRVGFDGPAA"
     gene            complement(3563..3715)
                     /locus_tag="VD788_16440"
     CDS             complement(3563..3715)
                     /locus_tag="VD788_16440"
                     /inference="COORDINATES: protein motif:HMM:NF012554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXV77901.1"
                     /translation="MGATDPAKAAAGTLRKMFASNIERNAVHGSDAPETAASEIAYFF
                     PGIALV"
BASE COUNT          684 a         1329 c         1261 g          452 t
ORIGIN      
        1 gcggctggtc gtccgcgcgg ttcacacgca atcgcgagca ctggatgctg gccggtctgg
       61 ccggtctgac cgggttgctg ttgtggatcg cctatttcgc gccgcagggg ctgctcaacg
      121 cctggatcgg gcattggtga acaggtaggc cgtcttgctt ccgaccggcc gtttcaccag
      181 cggttcgatc gaccccagtg cgtcccgcac ctcgtcgagc tgaccgtcct cgaccacgag
      241 gaacaccggc tcgctcgact cccagtaggc gcgcaacgcc gccggtgtct cgatccgcgg
      301 aatctcgcgg ccgagatagt agatgtaacc cgcgcgccac tcccagccgc gataggaacg
      361 aatggcggcc gcatctccgg tgatcgatcg cacctcctcg cagaacggcc gcgacgactt
      421 caacgggtcg agcgccggca gtgcccaggt cgccgccagc aggtagagcg cgaccacggt
      481 cgcgaacagc gccgccggcg ccagcgccgg acgggcccgt gcgcgtgcca gcgaccacgc
      541 ggcagcgagc gcgccgaccc cgagcacgac ggccgccgcg gtcaacggct gtgcgagcgt
      601 cgggtatcgc acgcggagca ccagccacgc gtacccaccc aagagcggca gcgcgaccgt
      661 catcgcggcg gcgatcagcc acagcgccgc ccgacggacg ggatccagcg ttcggtcgag
      721 ccaacgctcg accgttccgg cggcgagaat cgccacggcg ggggcgacgg gcaggatgta
      781 cgggctgcgc ttgctggccg acaacgagaa gaaggcgacg atcgtcagga tccaggcaac
      841 gcaccatcga tcgagcaatc ggaccgaggc ggcttccctc ccgctaaggc ggacgagcaa
      901 cagcaaaaag ccccagggcg ccatgtcgag ccaaaagtgc agcaggaagt accaccaggc
      961 acgttcgtga tcccacggcg cgaggaaacg ggtgacgttc tgtcgaacca gcaattcgtc
     1021 gaggaactcg tgttggccgc gaccgagcag aaccaacagc cacggaacgg cgacgaccgc
     1081 agcgacggcg gtggcgagca tcgtcccccg gaccgatggc agcacgcggg cgaccgccgc
     1141 gtgggccccg agggccagca acggacagat cacgccgacc ggtcccttgg ccaggaaacc
     1201 caggccgacc gacgcaccgc acagcgacca agccacgacc gccgtgcctc gctcgcgcat
     1261 cgcgcgcgtg ccggccaaca ccgcggccaa cgtcgtcacg ctcagcagca ggtccatctg
     1321 cacgctccgc gcgctccaga acacgaggta ggtcgtggcg aacagtgcgg cggcgatccg
     1381 ggcacggcga tgcccggcat gcgtcagcgc caagcggtag accagcgcca cgcccaccga
     1441 cccggccagc gcggacggca ggcgcagcga ccattcgctg actccgcccg cgacggtgga
     1501 cagaccgagc gccagccaga agtagagaat cggcttctcg tcgaagactc gaccattgaa
     1561 cgtcggaatc caccagtcgc cgcgctcgta catctcgacg acgacgcggc cgtacagcgg
     1621 ttcgttcgga ttccacagat cgcgcgcggc caagccgggc aggaacagca tcgccgacaa
     1681 cagcgcgaac gcggcgagct cgcgccgcga ccccgcgcgc cccgcaccac cgttcacgcg
     1741 gctggaccgt cgaagccgac acggtacaac cgggcgtaca gaccgccacg cgccagcaac
     1801 ccgtcgtgct cgccttgttc gacgatgcgc ccgccgtcga gaacgacgat ccgatcggcc
     1861 gcacggaccg tggcctcccg atgcgcgatg atcaaacagg tacggccgcg catcaaccgg
     1921 cggagggcgc ggcggacggc ggcctcggaa cgcgggtcga gcgccgacgt cgcttcgtcg
     1981 aggatcagca acggcgggtc tttgaccaac gcgcgtgcga tcgcgatccg ctgtcgctga
     2041 ccgctcgaca gcctggcgcc gcgctcgccg agcggcgtgt cgtagccgcg gggcagtcgc
     2101 gcgatgaaat cgtgcgcgtc cgcggcgcgc gccgcggcga tcacggcctc gagcggcacg
     2161 tcgtcgcggc cgtaggcgat gttatcccgg accgagtcgc cgaacagcat gctgtcgtgc
     2221 gtgaccaagc cgatctggtc tctcagtgac ttcaaggtca cgcgacgcag gtcgtgcccg
     2281 tcgatttcga tcgccccgct gaccgggtca tcgaagcgca acagcaggcg ggccagcgtc
     2341 gacttgccgg atccggacgg gccgaccagc gccagcgtcg tcccccgcgc gatcgtcaga
     2401 tcgatccgat cgagcactcg cccgtcgccg tagtcgaacg agacgtggcg gaactcgacc
     2461 gaagcggaga acggcgggag cgcccgcgcg ccggcggcat cgacgatccg cggccgctgc
     2521 tcgaccagct cgaacacgcg cgccgcggcg gcgacggcgc gttgggcctc ggcgtagacc
     2581 gtgttgagtc gccggatcga cgtgaacaac agcccgaggc tgaacagcac gacggtgaag
     2641 cggcccgcgt cgagcgcacc ccggtggatc gcctgaccgg cggcggcgaa cagtcctgct
     2701 ccgacgacgg cccccaacca ctccatgatc gacggtgcca gcgcctgggc ccgaccggct
     2761 cgaagctcgg cgcggaccag ctgggccagc gtccggcgaa agcgatcgat ctcgtgggcc
     2821 tccatgccga acgactgtac gacctcgatg ccggcgaccg attctgcgac gcggtcggcg
     2881 acgaaggcca tctgctcttg cgacgcggcc gccgcgcggc gcaaccggcg tccgaggcgc
     2941 agcatcggcc agcccagcag cggtagcgcg acgagcatca ccagtgacag tcgccattcg
     3001 tggtagagcg cggtggccag caagaacggc accatcgtgc cgacgcggac gagatccgcg
     3061 agctggctcg tcacgattcg atgcagacgt tggacgtcgc cgaggatgcg cgagaggatc
     3121 agccccgacg tgtgcgcgcg gaagaagacc agcgactgcc cgatcacgga gccgtagagc
     3181 gccagtcgca gatcgcggat gacgagtgcc cccgttcgca cggtgagata ctggccgagg
     3241 tacagcaccg ctccacgaac gaggaagatg gccacgatga tcgcggggac ggtcaggtac
     3301 ggacgccggt cgatccatcc cgacagcggc cgtggaagct cgaccgtcgg cgcaccgccc
     3361 ccgccatcgg tccagccgac cgacggaagc aacacgcggt cgaccaacgg ttcggcggcc
     3421 gacacgacgg ccgccatcag cgccccgccg acgccgagca gcccggccgc caggaccatc
     3481 gagccgaggt acggccggag gtagagcagc aggcgccgga aatcgcgcat acggtccgaa
     3541 tgctaacaga ccgcggcagg cctcaaacga gagcgattcc cgggaagaag taggcgattt
     3601 cgctcgcggc cgtttccggc gcgtccgatc cgtggacggc gttgcgctcg atgttgctcg
     3661 cgaacatctt gcgcaaggtg ccggcggccg ccttcgccgg atcggtcgca cccatcgtct
     3721 cgcgcc
//
LOCUS       DASPGN010000571         2839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1159861, whole genome shotgun sequence.
ACCESSION   DASPGN010000571 DASPGN010000000
VERSION     DASPGN010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159861"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1098)
                     /locus_tag="VD788_16445"
     CDS             complement(40..1098)
                     /locus_tag="VD788_16445"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HXV77902.1"
                     /translation="MREQRPEHLASGCLLVGLPAGDWTPRVGQRIEALDPAGVILFGR
                     NLVSAERTREMLGRIAERLTRPPLLAIDQEGGRVSRLERWIGPTVAARSLAALDESAA
                     RGFGEATGRALRWLGLNLDFAPVVDLSAVDATNGIGDRSFGTDPRLVTRMARAFLGGL
                     QGAGVAGCLKHFPGLGHTAIDSHDALPTVRRPLERLREEDLLPYRELGDLPASVMVGH
                     GHYPAFDGETPLPATLSPAIVGGELRGRLGFGGLVVSDDMEMGAVAGRDGEGAAAVAA
                     VRAGCDLLLYCSDLDRAETAARALAAAARADDELGDRLLRASERVGRFAERWHGVTSP
                     PELAEWRGCVAAFEAFRA"
     gene            1272..2198
                     /locus_tag="VD788_16450"
     CDS             1272..2198
                     /locus_tag="VD788_16450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXV77903.1"
                     /translation="MWSKLSSRLPRIGPGAKARFYTILILPHARSRFRKLHLSRAFVA
                     TCAMIALGVVGAGLYAPHLLLQLRSTEAAAERLQHENARLRAERDRFERTLAEIAGRL
                     DQFESRSVKIADALGVDELPSSRPAAGGPVTGLRGPRRSLLDDELDALKSRSDTLDGS
                     MDEIVLAFEERVRLLGSTPSILPAEGWFSHGYGWRKDPFTGNRQFHRGVDIVTDAGAP
                     IVATADGIVSRAVRAADYGKTVDLSHGFGYVTRYGHMSEILVRPGQRVRRGDVIGRVG
                     STGRSTGPHLHYEVFRDGRRVNPWKYLGQPGK"
     gene            2248..>2839
                     /locus_tag="VD788_16455"
     CDS             2248..>2839
                     /locus_tag="VD788_16455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV77904.1"
                     /translation="MDPDDRVDLDPATIADPLGEDDDQDGGADVDNSAPETVVDGAAP
                     DPESSSLVPRVGRALAPFDPFRRYMAEIKKYPPLSREEEQRLSRLYRESGDREALFRL
                     ITSNLMLVVRIALSFRRAARNLLDLIQEGNVGLLQAIDRFDPEFGVRLPTYAAYWVRA
                     YMVKFLLDNVRMVRVGTTNARRKLLRNLRKEKARLES"
BASE COUNT          438 a         1005 c          984 g          412 t
ORIGIN      
        1 atattccgca ttctgaccgg gcgggcgttg gcctgaggat caggcccgga aggcctcgaa
       61 cgcggcgacg cagccgcgcc actcggcgag ttcgggaggc gacgtgacgc cgtgccaccg
      121 ctcggcgaag cggccgacgc gctccgacgc gcgtagcagc cgatcgccca gctcgtcgtc
      181 cgcgcgcgcg gctgcggcca gcgcccgggc ggccgtctcg gcgcgatcga ggtccgagca
      241 gtagagcaac aggtcgcacc cggcccgcac ggccgcgacc gcagcggcgc cctcgccgtc
      301 gcggccggcg accgccccca tctccatgtc gtcgctgacg acgagcccgc cgaagccgag
      361 ccggccgcgc agctcgccgc cgacgatcgc cggcgacagc gtcgcgggca gcggcgtctc
      421 gccgtcgaag gccggatagt gaccgtggcc gaccatcacg ctggccggca gatcgccgag
      481 ctcgcgatag ggtaggaggt cctcctcccg caggcgctcg agcgggcgac ggacggtcgg
      541 cagcgcgtcg tgcgagtcga tggcggtgtg gccgagaccc ggaaagtgct tgaggcaccc
      601 ggcgacgccg gctccctgca gtcctccgag gaaggcgcgc gccatcctcg tcacgagtcg
      661 cgggtccgtg ccgaacgatc gatcgccgat cccgttcgtg gcgtcgacgg ccgacagatc
      721 gacgaccggt gcgaagtcga ggttcaagcc gagccagcgc aacgcacgcc ccgtcgcctc
      781 cccgaagccc cgcgctgccg actcgtcgag tgcggcgaga ctcctcgccg cgaccgtcgg
      841 gccgatccag cgttccaacc ggctgacccg ccccccctct tgatcgatcg ccaacagcgg
      901 gggccgcgtg agccgctcgg cgatgcggcc gagcatctcg cgcgtgcgct cggcgctgac
      961 gaggttccgg ccgaacagga tcacccccgc cggatcgagc gcctcgatcc gttggccgac
     1021 ccgaggcgtc caatcaccgg cggggagccc gaccaggaga cagccggaag ccagatgctc
     1081 ggggcgttgc tcgcgcatcg cctgtcatcc tgggctccgg cggggcgatt ggcaacagcc
     1141 ggaaaaagaa agaggaaaaa cggggcgatc gaacgtattt gacctgtgag ggggccggat
     1201 gttaacttcg cttggcgacc ggacgcggaa agtcgtgtgg aagtcgtgcc aacgcaaggg
     1261 gttcatcagt tttgtggtcc aagctcagct cgaggctacc gcggatcgga cccggagcca
     1321 aagcgcgttt ctacacgatc ctgatcctcc cgcacgcgcg cagccgattc cgtaagctcc
     1381 acctctcccg tgcattcgtc gccacatgcg cgatgatcgc gctcggcgtg gtcggggccg
     1441 ggttgtacgc gccccacctc ctcttgcagc ttcgctcgac ggaagccgcg gccgaacggc
     1501 tccagcacga gaacgcgcga ttgcgcgccg agcgagaccg cttcgagcgg accctcgccg
     1561 agatcgccgg gcggctcgat cagttcgaga gccgcagcgt caagatcgcg gacgcgctcg
     1621 gcgtcgacga gctgccgtcg tcgcgtcccg ccgccggcgg gcccgttacc gggctgcgcg
     1681 ggcctcgccg ctcgctgctc gacgacgagc tcgacgcgtt gaaatcccgc agcgacacgc
     1741 tcgacggctc gatggacgag atcgtgctgg cgttcgagga gcgggtccga ctgctcggtt
     1801 cgacgccgag catcttgccg gccgagggct ggttctcgca cggctacggt tggcgcaagg
     1861 acccgttcac cgggaaccgt cagttccacc gcggggtcga catcgtgacg gatgccggcg
     1921 cgccgatcgt cgcgaccgcg gacgggatcg tctcccgggc ggttcgcgcg gccgactacg
     1981 gcaagacggt cgacctgtcc cacgggttcg gctacgtcac gcgttacggg cacatgagcg
     2041 agatcctggt gcggcccggc cagcgcgtgc gccgtggcga cgtcatcggt cgggtgggca
     2101 gcaccggtcg ctcgaccggt ccccacttgc attacgaggt gttccgcgac ggccggcggg
     2161 tcaacccgtg gaagtacctc gggcagcccg gaaagtgagg tttccggctc cctgccggcg
     2221 gattttgcgc gtggtaacct gcaggggatg gaccccgacg atcgcgtaga cctcgatccg
     2281 gcgacgatcg ccgatcccct cggcgaagac gacgaccaag acggcggggc ggacgtcgac
     2341 aactcggcgc ccgagaccgt cgtcgatggc gcggcccccg atccggagag ttcctcgctc
     2401 gtcccccgcg tcggtcgcgc gctcgcgccg ttcgacccgt ttcgccgcta catggcggag
     2461 atcaagaagt acccgcctct cagccgggag gaggagcagc gtctctcgcg cctctaccgc
     2521 gagtcggggg accgcgaggc gctgttccgc ctgatcacct cgaacctgat gctcgtcgtg
     2581 cggatcgcgc tcagcttccg gcgggcggcg cggaacctgc tcgatttgat tcaggaaggg
     2641 aacgtcggcc tgctccaagc gatcgaccgg ttcgacccgg agttcggtgt ccgcctcccc
     2701 acctacgcgg cgtactgggt ccgggcctac atggtgaagt tcctgctcga caacgtccgc
     2761 atggtccgcg tcgggacgac gaacgccagg cgcaagctgc ttcgcaacct ccgcaaggag
     2821 aaggcacggc tcgagagcc
//
LOCUS       DASPGN010000572         5870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_809900, whole genome shotgun sequence.
ACCESSION   DASPGN010000572 DASPGN010000000
VERSION     DASPGN010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809900"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5870)
                     /locus_tag="VD788_16460"
     CDS             complement(<1..>5870)
                     /locus_tag="VD788_16460"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXV77905.1"
                     /translation="GPRSSLTLWQGAIDVLRAVDVKDAANVHVLFAGGVHDALSAAMV
                     STLAVPLAERGIKVGVLMGTSYLFTTEAVASGAITRSYQLEAIACRRTALLESGVGHA
                     TRCAATPFAGEFAACKRELQAAGLASDEIRLRLELLNVGRLRVASKGVTRKAKTEREA
                     LDPDSTAELVEVDADTQRREGLYMIGALAALRDDTLSMGDLHRQVAEGGGELLRSLAA
                     RDGSADVRPVAVRHGEPIAIVGIACLFPGAASLDRFWRNILDGVDTIRDVPEERWRRD
                     QFYHEQRRTPDHLYATRGAFLEPIRFDPLRYMIPPATLASVEPAQLLSLEVTRRALAD
                     AGYDRRPFDRDHTSVIIGCGSIHDLGIHYVQRTLIPQLVASVPGLDDAQRERIVTGVR
                     DRLAPWTEDSFPGVLPNVIAGRIANRLDLRGANFIVDAACSSSLAAIHTAVEQLRSGT
                     SDVVLAGAVDCSNNAFTFMGFAQTQALTAGARPRPFDASADGIVLGEGVATLVLKRLA
                     DAERDGDSIYAVIRGIGCSSDGRNTSLTAPFAEAQALALRRAYDDAGIDPGTVGLIEA
                     HATGTALGDRAEIESLIEVVGRTGARAHGCAIGSVKSMIGHTKTSAGMAGVIKAAMAL
                     KQRVLPPTINVERPNPALGSDEALYLNTETRPWIGSPGDHPRRAGVSAFGFGGTNFHV
                     VLEEYTGGYHERSALDRAPRPVEVMSWARSNRAELAAELSTVRDSIAGLAELDLARLA
                     QTLAAEERRRAAPGPGPVRAAVVAESVRDLAGKLDRLIAALAGTDPLAERTGVFLSDE
                     PPVGRDEVCFLFPGQGAQYPGMLKELAQDAPFGPSMFEEADAMLSDLVPGRLSERIFP
                     RPTFDKQERAAQQQALTDTRIAQPAIGVVDLFALDVLARFGIEPAMVAGHSYGESVAL
                     AAAGSFTRPGLLRLSAWRGLVLHEVARRNPGGMAAVAADEATTREGLAAIGSPASIAN
                     ANGPRQTIIAAAPAELDAAIRGLTERGIAVRRVAVSAAFHTSAMDEASASLRQNLAAT
                     PMWVPRIPVYSNVNAEVYPQQVEAVRDLLTRNMTSCVRFVDQVRAMYRDGARLFVEVG
                     PGRILTGLVARILEDRPHLALALDQPGQSAWSSLGHLLARLHAAGLAVDWEPWFTGRC
                     PGDETLATCVARARTEHEGRPTDWWVGPGGSRPVRSLAGGEPRKAARAAAPEPSAVTD
                     EDRERRSDTMSEDRRDRLVRLHREAMSQWLDLQREQVRLNERFLRLQERLVAAELGLP
                     QPAFDEDDGRPAPAPTAAVGPAAGLSVAPAPILPRLEPTADAAPVTAAERKPEAAVPA
                     SVVPVAAASAEIDAIGGPPTTSAFYRDLLAEVSRRTGYPEEMLDADLPLEAGLGIDSI
                     KVMEIFSALKPYHRILASNDEQESEDVLAQFVELKTLGAIREHYERRRAEVLTGTPGA
                     ALPASAPVGTERPSGGQVPVDRLVVRSVPSNALAEVRIDADALRFSREHVLLLLGDVA
                     DYGPGLRAALARGGYRALQVVPGQATRRVADLRYEADLDDPRSLAGLAAAIRDECGVP
                     VGGLVNLLGLADGFRRTDLDAPDAPLQLATRLLNVAKTFESDILASVEAGGGIVVNLT
                     GMDGRFGLSDSRPLPVAQAASVGFFKSLAREWPGVRVKNIDVDTDAAPEVLLTAIVAE
                     IANWDELLEVGINAEGRWSIDLVDEAAGQSTEEDARQAARALGSWTSGLTLDADSVVL
                     ATGGARGITAEVVRLLAQETSTRLVIVGRSPLSAVEDEPQELRDLAGTAELRQALIRS
                     RGADGGSTPAQIERQVEALQRDRAIRSNLAAFAAAGSRVDYHAIDVRDPHALAALVEQ
                     VYQRYGRIDGVIHGAGVVEDRRLRDKTAESFARVFQTKVRPAMVLAGALRPESLQFLV
                     FFSSVSGRFGNAGQADYSAANECLNKLAAHLDRVWSARVVAMNWGPWDCGLISEAL"
BASE COUNT          829 a         2149 c         2071 g          821 t
ORIGIN      
        1 gcaacgcctc ggagatcaga ccgcagtccc acgggcccca gttcatcgcg acgacgcgag
       61 ccgaccacac gcgatcgaga tgcgcggcca acttgttcag gcactcgttg gccgcgctgt
      121 agtcggcctg gcccgcattg ccgaagcgtc ccgacaccga cgagaagaag acgaggaact
      181 gcaacgactc cggccgcagc gcgccggcca gcaccatcgc cggccggacc ttggtctgga
      241 agacacgggc gaacgactcg gcggtcttgt cgcgcaaccg gcgatcctcg accaccccgg
      301 cgccgtggat cacgccgtcg atccgaccgt agcgctggta gacctgctcg acgagcgcgg
      361 cgagcgcgtg cgggtcgcgg acgtcgatcg cgtggtagtc gacccgcgag cctgccgcgg
      421 cgaacgcggc cagattcgac cggatcgccc ggtcgcgttg gagcgcctcg acctgccgct
      481 cgatctgtgc cggcgtgctg ccgccgtccg cgccgcgcga gcggatcagc gcctggcgca
      541 gctcggcggt cccggcgaga tcgcgcagct cctgcggctc gtcctcgacc gccgagagcg
      601 gcgatcgtcc gacgatcacc aggcgcgtcg aggtctcctg ggccagcagc cgcaccacct
      661 cggcggtgat gccacgcgcg ccgccggtcg ccagcacgac gctgtcggcg tcgagcgtca
      721 atccgctcgt ccagctgccg agggcgcggg cggcctgccg cgcgtcctcc tccgtcgact
      781 gccccgccgc ttcgtccacc aggtcgatcg accagcgtcc ctcggcgttg atcccgacct
      841 cgagcaactc gtcccagttg gcgatctccg ccacgatcgc cgtcagcagc acctccggcg
      901 ccgcatccgt gtcgacgtcg atgttcttga cccgtacgcc gggccactcg cgcgccagcg
      961 acttgaagaa gccgacgctc gccgcctgcg cgaccgggag cgggcgcgaa tcggacaggc
     1021 cgaatcgacc gtccatcccg gtcaggttga cgacgatgcc gccgccggcc tcgacgctgg
     1081 ccaggatgtc gctctcgaac gtcttcgcca cgttgagcag ccgggtcgcg agctgcaacg
     1141 gcgcgtcggg cgcgtcgaga tcggtgcggc ggaaaccgtc ggccaagccc aacaggttga
     1201 ccagcccgcc gacgggcacc ccacactcgt cgcgaatcgc ggcagccagt cccgcgagag
     1261 atcggggatc gtcgagatcc gcctcgtaac gcaggtcggc gacccggcgc gtcgcctggc
     1321 ccggcacgac ctgcagcgcc cggtagccgc cgcgcgcgag cgccgcgcgc aggccgggac
     1381 cgtagtccgc cacgtcgccg agcagcagca ggacgtgctc tctcgagaag cgcaacgcgt
     1441 cggcgtcgat gcgaacctcc gcgagcgcgt tggacggtac ggagcgcacg acgagacggt
     1501 cgaccggcac ctggccgccg ctcgggcgct ccgtccccac cggggcggac gccggaagcg
     1561 cggcgccggg cgtaccggtg agcacctcgg cccgtcgtcg ctcgtagtgc tcgcggatcg
     1621 cgccgagggt cttcagctcg acgaactgcg ccagcacgtc ttcgctctcc tgctcgtcgt
     1681 tcgacgccaa gatgcggtgg tacggcttga gcgcgctgaa gatctccatc accttgatcg
     1741 agtcgatgcc gagtccggcc tcgagcggca agtcggcgtc gagcatctcc tcggggtagc
     1801 ccgtccgccg gctgacctcc gccagcaggt cgcggtagaa cgcggaggtg gtcggcggac
     1861 cgccgatcgc gtcgatctcg gcggacgcgg ccgcgaccgg cacgaccgaa gcgggtacgg
     1921 ccgcctccgg cttgcgctcg gccgccgtga ccggcgcggc gtctgcggtc ggctcgagcc
     1981 gtgggaggat cggtgccggc gcaaccgaca gccccgccgc gggtccgacg gccgccgtcg
     2041 gagcgggcgc gggtcgcccg tcgtcctcgt cgaacgcggg ttggggcaaa cccagctccg
     2101 cggcgaccag ccgctcctgc agacgcagga agcgctcgtt gagtcggacc tgctcgcgct
     2161 gcaggtcgag ccactggctc atcgcttcgc gatgcagccg aaccagtcga tcccgtcggt
     2221 cttcgctcat cgtgtcgctt cttctctccc ggtcttcatc cgtcaccgcg gacggctcag
     2281 gcgccgccgc tcgggccgcc ttacgcggct cgccaccggc gagcgatcgg accggccggg
     2341 agccgcccgg tccgacccac caatcggtcg gccggccttc gtgttccgta cgcgcccgcg
     2401 cgacgcacgt cgcgagagtc tcgtcacccg ggcagcggcc ggtgaaccac ggctcccagt
     2461 cgacggcgag gccggcggcg tgcagccggg ccagcaggtg accgagcgag ctccaggccg
     2521 actgccccgg ctggtcgagc gccagcgcca ggtgcgggcg atcctcgagg atcctcgcga
     2581 cgaggccggt caggatccgt cccggtccga cctcgacgaa cagacgtgct ccgtcgcggt
     2641 acatcgctcg cacttggtcg acgaaccgga cgcaactcgt catgttccgc gtcagcaggt
     2701 cgcgcaccgc ttcgacctgt tgcggataga cctcggcgtt gacgttgctg tagaccggga
     2761 tcctcggcac ccacatcggc gtcgccgcca ggttctggcg caggctggcc gaggcctcgt
     2821 ccatcgcgct cgtgtggaac gcggcgctga ccgcgacgcg ccggaccgcg atgccgcgct
     2881 cggtcagacc gcggatggcg gcgtcgagct cggcgggagc ggcggcgatg atcgtctggc
     2941 gcgggccgtt cgcgttggcg atcgacgccg ggctgccgat cgcggccagc ccctcgcgcg
     3001 tcgtcgcctc gtcggccgcg accgccgcca tgccgccggg gttgcgccgc gcgacctcgt
     3061 ggagcacgag accgcgccag gccgacaacc gcagcagacc cgggcgggtg aagctgccgg
     3121 cggcggcaag cgcgacggac tcgccgtagc tgtgaccggc gaccatcgcc ggctcgatgc
     3181 cgaaccgcgc gagcacgtcg agcgcgaaca ggtcgaccac cccgatcgcg ggctgcgcga
     3241 tccgcgtgtc ggtcagtgcc tgctgctgtg ccgctcgttc ctgcttgtcg aaggtcgggc
     3301 gcgggaagat ccgttcggac aagcggcccg ggacgagatc ggacagcatc gcgtccgcct
     3361 cttcgaacat cgacgggccg aacggcgcgt cctgggccag ctccttcagc atgccgggat
     3421 actgcgcacc ctgtcccggg aagaggaagc aaacctcgtc gcggccgaca ggcggctcgt
     3481 cgctgaggaa caccccggtc cgctcggcga gagggtcggt cccggcgagc gcggcgatca
     3541 gccggtcgag cttgccggcc aggtcgcgca ccgactcggc gacgacggcc gcccgcaccg
     3601 ggcccggccc gggcgccgcg cgccggcgtt cctcggcggc cagcgtctgc gccagccggg
     3661 cgagatcgag ctcggcgagc ccggcgatcg agtcgcggac ggtgctcagc tcggcggcca
     3721 gctcggcccg gttgctcctg gcccagctca tcacctcgac cggccgcggc gcgcgatcga
     3781 gcgcggaccg ctcgtgatag ccgcccgtgt attcctcgag cacgacgtgg aagttcgttc
     3841 cgccgaagcc gaacgcgctg acccccgccc gacgcgggtg gtcgcccggc gagccgatcc
     3901 acgggcgggt ctcggtgttg agatagagcg cctcgtccga cccgagcgcg ggattcggcc
     3961 gttcgacgtt gatcgtcggc ggcaacaccc gctgcttgag cgccatcgcg gccttgatca
     4021 ccccggccat gccggccgac gtcttggtgt ggccgatcat cgacttgacc gagccgatcg
     4081 cgcagccgtg cgcacgcgcg ccggtccgcc cgacgacctc gatcaggctc tcgatctccg
     4141 cgcggtcgcc gagcgcggtt cccgtcgcgt gcgcctcgat cagcccgacc gtaccggggt
     4201 cgattcccgc gtcgtcgtac gcgcggcgca gcgcgagcgc ctgcgcctcg gcaaacggtg
     4261 cggtcaaact cgtgttgcgc ccgtcgctcg aacaaccgat gccgcggatc acggcgtaga
     4321 tcgaatcgcc atcgcgctcg gcgtcggcca gtcgcttgag caccagcgtc gcaacgccct
     4381 cgcccagcac gatgccgtcg gccgaggcgt cgaacggtcg cgggcgcgct ccggccgtca
     4441 acgcctgcgt ctgggcgaag cccatgaacg tgaaggcgtt gttcgagcaa tcgacggccc
     4501 cggcgagcac gacgtcgctg gtaccgctgc gcagctgctc gaccgcggta tgaatcgccg
     4561 ccagcgagga cgagcacgcc gcgtcgacga tgaagttggc gccgcgcagg tcgagtcggt
     4621 tcgcgatgcg cccggcgatc acgttgggca gtacgccggg gaaactgtcc tcggtccacg
     4681 gtgccaggcg atcgcgtacg ccggtcacga tccgctcgcg ctgcgcgtcg tcgagccccg
     4741 gcaccgacgc gacgagctgc gggatcagcg tgcgctgcac gtaatggatc cccaggtcgt
     4801 ggatgctgcc gcagccgatg atgaccgacg tgtggtcgcg gtcgaacggc cggcggtcgt
     4861 agcccgcgtc ggccagtgca cgacgcgtga cctcgagcga caacagttga gccggctcga
     4921 cgctggccag ggtggcgggt gggatcatgt aacgcagcgg atcgaagcga atcggctcga
     4981 ggaacgcgcc gcgcgtggcg tagaggtgat ccggggtgcg acgctgttcg tggtagaact
     5041 ggtccctgcg ccagcgctcc tcgggtacgt cgcggatcgt gtcgacgccg tcgaggatgt
     5101 tgcgccagaa tcgatcgagg ctcgccgcgc cggggaacaa acaggcgatg ccgacgatcg
     5161 cgatcggctc gccgtggcgg accgcgaccg gtcggacgtc ggcggatccg tcgcgcgcgg
     5221 cgagcgaacg gagcagttcc ccgccgccct cggccacctg gcggtgcaga tcgcccatcg
     5281 acaacgtgtc gtcgcgcagc gcggcgagcg cgccgatcat gtacagaccc tcgcggcgct
     5341 gggtgtcggc atcgacttcg acgagctcgg cggtcgaatc gggatcgagc gcctcacgct
     5401 cggtcttggc cttgcgggtc acgcccttgg atgccacgcg cagccggccg acgttgagca
     5461 gctcgaggcg cagccggatc tcgtccgacg cgaggcctgc ggcctgcagc tcgcgcttgc
     5521 acgcggcgaa ctcgccggcg aacggcgtgg cggcgcagcg cgtcgcgtgc ccgacgcccg
     5581 attcgagcag cgccgtgcgc cggcaggcga tcgcctcgag ctggtagctg cgggtgatcg
     5641 ccccgctcgc gacggcctcg gtggtgaaca gatacgaggt gcccatcagc acgccgacct
     5701 tgatgccgcg ctcggcgagc gggacggcga gcgtcgagac catcgcggcc gacagggcgt
     5761 cgtgaactcc gccggcgaac agcacgtgca cgttcgccgc gtccttcacg tcgacggcgc
     5821 gcagcacgtc gatcgccccc tgccacagcg tcaggctcga tcgcggtccg
//
LOCUS       DASPGN010000573        10241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_810165, whole genome shotgun sequence.
ACCESSION   DASPGN010000573 DASPGN010000000
VERSION     DASPGN010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810165"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VD788_16465"
     CDS             <1..281
                     /locus_tag="VD788_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77906.1"
                     /translation="QGLCSTADPGPPVVDSLSGSTYPFPLQLPKGGTVTVKAIRSDVA
                     PPGVAGFSPAGGPVVSAQLLVNGTPVSVDGTTTSPNSQTGISFSYSSR"
     gene            344..1519
                     /locus_tag="VD788_16470"
     CDS             344..1519
                     /locus_tag="VD788_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77907.1"
                     /translation="MKIDRSAFVGCLILWAAGLHPVGAQDDAAAPEKNLQFFLEIDAW
                     NAQPLGLEYEPATVGDPDSVVGPEIIEFEQEPETAVYYRAGISLGPNVGEFKASWYEQ
                     DQQNSLDQRTASIFGFGESLAYPLFAGYENDGLADGFTSTATTGYSELRFDFRRIAFR
                     SDRLTGKWFVGVRRVTHDRALNVDYYALAPSPPTVPPTSVPRPDLLPQPDQASISSEF
                     IGRGIEGGMDFHVPLSGDRFALEAGFGVAVLRGHLDASYSSTNSLYLVEETEGVFVVA
                     KAPYDYFYDPSGTGLVVDNVTQASSTFRLTADSRTTVSPVIDLYLGFRARIWKALELV
                     VGWRSTYYGDVGVDLRPKSFTVVPTSDDTTAVNFVDVDEVDRSVDYQGAYLGVAFSF"
     gene            1565..2740
                     /locus_tag="VD788_16475"
     CDS             1565..2740
                     /locus_tag="VD788_16475"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HXV77908.1"
                     /translation="MPLDKQRLLDRLRGLAFPGYSRDVVSLGVVRDLAIDGTVVEVVV
                     DGAELAPAAREQLERGIRATLSAVDGVDEVRVRDVKSAGSPALKVVGSRPPRGVAAGG
                     GVDRGLIPDVRRVIAVASGKGGVGKSTVAVNLAVALAAEGLRVGLLDADIYGPSIPLM
                     FGLAGERPSFDDESRRLLPFDRCGVRLMSLGFLIEDADTAVIWRGPMVMKALEQLLGE
                     VAWGALDVLVVDMPPGTGDAQLTMSQRVQLAGAVIVTTPQDVALADAIKGINMFRKVG
                     VPLLGLVENMSYFRCPHCGERSEIFSHGGGRVQAERLDVPFLGEVPLDATLRASGDDG
                     RPVVDAAPASALAGVFRAIARDVARSLALGPPDGEPATDAAGVFDRFRRLWKDPGSA"
     gene            2821..3669
                     /locus_tag="VD788_16480"
     CDS             2821..3669
                     /locus_tag="VD788_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77909.1"
                     /translation="MAGVRGLAVWIVIGASFAARAADDPLAIINDAKAKAVTIPKQAE
                     ALAALAWPAGEVDPAVSSLAREQLVGYGDRGLDAMIGRFRVAPARFAADITSAFLETR
                     LVVRSNQPPSFIPSLVEILWFGPPEAKRLALPELAARRFGPAMLSFIDAAYEHPELTG
                     PVISALGRLRDDRARHYLGEILLRADSTYTRPAAEALARIGGRAIDVLREASASPDEL
                     VRGSAIDALVPLSGVTDLTILYEYVAQFPDDDPRRIELVIQRATQLETLLEARQDVDA
                     GSATDF"
     gene            complement(3673..4809)
                     /locus_tag="VD788_16485"
     CDS             complement(3673..4809)
                     /locus_tag="VD788_16485"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXV77910.1"
                     /translation="MTLRISARMDGIDRTLIRRIFDAAPPGAINLGLGEPGLPTPDPC
                     ALAGIATIAEGRTGYTSTAGDPLLRAAVAREYPGFADGPESVCITVGSQEAMFASCLV
                     LLDAGDELLYPDPGYPAYPVVARMLGATATPYPLDPSRRFRLDAAAVLDRVGERTRVV
                     VLCSPSNPTGAVAEPAAVEALTRALAARGVAWLSDEVYAGFVWDRAFASPASYSNGGG
                     LVVAGLSKGMSMTGWRVGWVVGPPAVVARVTAAHQYLLTCASTISQGAALAAFTPAGR
                     RARARHAELLASRRELMADELQGIAGLEFHRPDGAFYYFVDVRAHGDSVALARRILER
                     QAVIVIPGEAFGERGAGWLRVSFAAEPERIREGVGRIRAELTRR"
     gene            complement(4806..5639)
                     /locus_tag="VD788_16490"
     CDS             complement(4806..5639)
                     /locus_tag="VD788_16490"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HXV77911.1"
                     /translation="MSLGLRGEIESLIEAERLDTAAARAVIERLFDALEEGRVRAASP
                     GDGSWVVNPWVKQGILLAFRIGRNVRSGAADGLVFHDRDTLPLWRPRDGSEVRVVPGG
                     SSVRRGAHVADGVVIMPPSYVNVGAFVGAGSLIDSHVLVGSCAQVGRDVHLSAAVQLG
                     GVLEPIGTLPVIVEDGAFLGGGCGIYEGVRIGRRAVLAPGVVLSRAVALYDLPRRAVH
                     RAGEDGSLVVPDGAVVVPGARPATGEFAAEHGLKLHAPVIVKYRDARTDAALALEGAL
                     R"
     gene            complement(5636..6550)
                     /gene="dapA"
                     /locus_tag="VD788_16495"
     CDS             complement(5636..6550)
                     /gene="dapA"
                     /locus_tag="VD788_16495"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXV77912.1"
                     /translation="MMANHGLASTLSGAMTALITPFDGAGKIDRAALCRLVAWQVEQG
                     IDALVPCGTTGEAATLGEDEKAEVVAATVDAAAGRVPIIAGCGTNNTRTTVDGAKRCA
                     AAGADALLVVTPYYNKPNASGMLAHYEAVAGATALPVIVYNVPGRTGQNLGVELTLAL
                     AEIPGVIGVKEASADLEQIARILAARPPGFVVLSGDDPLALPTVALGAEGVISVVANE
                     APAEMARLVAHARAGELQPARELHYRLLPLMRANFVESNPVPVKTAMHRLGFCELVLR
                     APLGPPQAATVDAVTEALRRAAVTAGAR"
     gene            complement(6577..7335)
                     /locus_tag="VD788_16500"
     CDS             complement(6577..7335)
                     /locus_tag="VD788_16500"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HXV77913.1"
                     /translation="MRYLLVGAGRMGRAIEAEAAGRGHTCVGRLRSVDAARLAPGRVD
                     RTAMGVPEVAFEFTEPSSARRNVLALTGAGVAVVCGTTGWDPDDAFHREIAGRAAGAI
                     VAANFSLGMQLFCRILGHAAGLFAAADSHRPSIFEMHHTGKLDAPSGTARALARILRE
                     RDPRLRSVVEGNPERRLPDGALHVASLRVEFEPGTHVVGFDSDHDRIELTHRARGRSG
                     FAAGAVLAGEWIAGKTGLHGFDEVVDELVRAARR"
     gene            complement(7335..8687)
                     /locus_tag="VD788_16505"
     CDS             complement(7335..8687)
                     /locus_tag="VD788_16505"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXV77914.1"
                     /translation="MKFGGTSVADADRIRSVAEIVGRARDRAPLVVVSALAGVTDTLE
                     RLVGAALGGDPDAVEPLLADLERRHRWAMSGLEQTAKERHHLSVEIDGLFDQLRQKLR
                     SIRILGERSPRTADAILATGETLSSTIVTRALVEAGQPATWVDPRRVVVTDDRFGQAE
                     PNAAGVAERARRLLEPLIRGGRIPVLGGFVGATVGGETTTLGRGGSDTSAAVLGAALG
                     ADEIQIWTDVDGMMSADPRLVPDARPIERLSFAEATELAFYGARVLHPDCLAPAVRRG
                     IPLRIRNSLRPAAPGTVVVGEVDSTEPERVVSVASRAGVRTVSVVNRKMRPDAAFAAA
                     VLAVFGECSVVPELVVASELAVHVVGSSSGDHGPLVAALERLGRVRVEPRRAVICIVG
                     SALRVGAERSRAVCALAAWQPELLALGSSGASLAAVVEEDRLIGAVRDLHRRYLEGAA
                     "
     gene            complement(8693..9763)
                     /gene="asd"
                     /locus_tag="VD788_16510"
     CDS             complement(8693..9763)
                     /gene="asd"
                     /locus_tag="VD788_16510"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXV77915.1"
                     /translation="MNRYRVGIVGATGLVGQRLVERLDRHPWFELRALAASDRSAGKP
                     YDEAANWQLPGGGPPAAFAQLRVRRCRVDELDDCDLVLSALDAEIARELEPAIAGAGI
                     PVVSNSSAFRQHAEVPLVVPEVNPSHLELIDGPRRRDGGYIVTNPNCSTTGLVVALAP
                     LHRAFGVSQVVVTTMQAASGAGLAGPTALQLLDNVLPHIPGEEQKMEREVGKILGRVE
                     AARIEPAPLVVSAHCHRVPTIDGHLEAVSVKLAVGADLDAVRGALLDFRGAIEPGSLP
                     SAKARPLLVRDEDDRPQPRLDRDEGSGMTVVVGRLRRCPVLDLRFVVLSHNTVRGAAG
                     GTLLNAELLAARELLPRRGGAA"
     gene            9853..10089
                     /locus_tag="VD788_16515"
     CDS             9853..10089
                     /locus_tag="VD788_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77916.1"
                     /translation="MSEPSEQNGEISVCGRGKYRAPEDDFYCLRYGVWYPSIDCAFRT
                     RYRTAPGCRDCEQGRFNLKRHARAIARPGRFRSG"
BASE COUNT         1508 a         3687 c         3557 g         1489 t
ORIGIN      
        1 ggcagggctt gtgctcgacg gccgatcccg gccccccggt cgtcgactcg ttgtcgggct
       61 cgacctaccc gtttccgctt caactgccga agggcgggac cgtcacggtc aaggcgatcc
      121 gcagcgacgt cgccccgccg ggcgttgccg gcttctcgcc cgcgggaggt cccgtcgtca
      181 gcgctcagct gctcgtcaat ggcaccccgg tgagcgtcga cggcaccacc acctcgccga
      241 attcgcagac aggcatcagt ttctcctact cgagcaggtg aacggcagcg gatgaccgag
      301 agatacacga cgggcgcgca caggcccggc cgaggtgtcc ccgatgaaga tcgaccgtag
      361 cgcgtttgtg gggtgcttga tcttgtgggc ggccggactt caccccgtcg gggcgcaaga
      421 cgacgcggcg gcgcctgaga agaacctgca gttcttcctc gagatcgacg cgtggaacgc
      481 ccagccgctg gggctcgaat acgagccggc cacggtcggc gatcccgaca gcgtcgtcgg
      541 tcccgagatc atcgagttcg agcaggagcc cgagaccgcc gtctactacc gcgccgggat
      601 cagtctcggg ccgaacgtcg gcgagttcaa agcgtcgtgg tacgagcagg accagcagaa
      661 ctctctcgac cagcgcacgg cgagcatctt cggtttcggc gaatcgttgg cctacccgtt
      721 gttcgcaggc tacgagaacg acggtctggc cgacggattc acgtcgacgg ccacgacggg
      781 gtattcggag ctccggttcg acttccggcg gatcgcgttt cgctcggacc ggctgaccgg
      841 caagtggttc gtcggcgtgc gccgcgtgac gcacgatcgc gcgctcaacg tcgactacta
      901 cgcgctggca cccagcccgc cgaccgttcc accgacgagc gtgccgcgac cggaccttct
      961 cccgcagccg gatcaggcgt cgatctcgtc ggagttcatc ggtcgcggca tcgagggcgg
     1021 aatggacttc cacgtaccgc tgagcggcga ccgcttcgcg ctggaggcgg gtttcggggt
     1081 cgccgtgctc cgcgggcacc tcgacgcatc ctactcttcg accaactccc tctatctggt
     1141 cgaggaaacg gagggcgtgt tcgtcgtcgc caaggccccg tacgactatt tctacgaccc
     1201 gagcggcacc ggactggtcg tcgataacgt gactcaggcg tcgtcgacct tccgcttgac
     1261 ggcggacagc cggaccacgg tgagtcccgt gatcgacctc tacctcggct tccgcgcccg
     1321 tatctggaag gccttggagc tggtcgtggg ctggcgctcc acctactatg gagacgttgg
     1381 cgtcgatctg cggccgaagt ccttcaccgt cgttccgacc tccgacgaca ccaccgcggt
     1441 gaacttcgtc gacgtggacg aggtcgatcg ctcggtcgat tatcagggcg cctacctcgg
     1501 ggtcgcgttc agcttctgaa cgagtccgcg ggccgcgctc gccgatgata tagtctccgc
     1561 atcgatgccg ctcgacaagc aacgactgct cgaccgactt cgtgggttgg cgttccccgg
     1621 ctattcgagg gacgtcgtct cgctcggcgt ggtgcgcgac ctcgccatcg acgggacggt
     1681 cgtcgaggtc gtcgtcgacg gtgcggaact cgctcccgcg gcgagggagc agctcgagcg
     1741 cgggatccga gcgacgctgt ccgccgtcga cggagtcgac gaggtccggg ttcgcgacgt
     1801 caagagcgcg ggatcgccgg cgctcaaggt cgtcggctcc cgcccgccgc ggggagtggc
     1861 cgccggcggc ggagtcgacc gcggactgat ccccgatgtc cgccgcgtga tcgccgtggc
     1921 ctccggcaag ggcggcgtcg gcaagtcgac cgtcgcggtc aatctcgcgg tggcgctggc
     1981 cgccgagggt cttcgcgtcg gtctgctcga cgcggacatc tacggtcctt cgattccgtt
     2041 gatgttcggt ctcgccggcg agcggccgtc gttcgacgat gagtcccggc gcctgctccc
     2101 gttcgatcgc tgcggcgtcc ggctgatgtc gctcggcttc ctgatcgagg acgccgacac
     2161 ggcggtgatc tggcgcggac cgatggtgat gaaagcgctc gagcagttgc tcggcgaggt
     2221 cgcgtggggc gcgctcgacg tcctggttgt cgacatgccc cccggaaccg gcgacgcgca
     2281 attgacgatg tcgcagcgcg tccagttggc cggtgcggtg atcgtgacga cgccgcaaga
     2341 cgtcgcgctg gccgacgcga tcaaggggat caacatgttt cgcaaggtcg gcgtcccgct
     2401 gctcggcctc gtcgagaaca tgagctactt ccgctgcccg cactgcggcg agcgcagcga
     2461 gatcttcagt cacgggggag ggcgcgtcca ggccgagcgc ctcgacgtgc cgtttctcgg
     2521 cgaggtcccg ctcgatgcga cgttgcgtgc ctcgggcgac gacggccggc ccgtcgtcga
     2581 cgccgcaccg gcctcggcgc tggccggggt gttccgcgcg atcgcccgtg atgtcgcgcg
     2641 ctcgctcgcg ctcggcccgc ccgacggaga gccggcgacg gatgcggccg gcgtcttcga
     2701 ccgattccgg cggttgtgga aagacccggg cagcgcttga tcgccgattc gtcgcgcggc
     2761 gacgctggcg gtatattctc tgcggtcgtc ccgcggcggg cgactgtgag gaggatgacg
     2821 gtggcgggcg tgcgcgggtt ggcggtgtgg atcgtgatcg gcgcctcgtt cgcggcgcgc
     2881 gcggcggacg acccgctcgc gatcatcaac gacgcgaagg ccaaggccgt cacgatcccg
     2941 aagcaggccg aggcgcttgc cgcgctcgcc tggccggcgg gtgaggtcga tcccgccgtc
     3001 agctcgctgg cacgggagca gctcgtcggc tacggcgacc gcggcctgga cgcgatgatc
     3061 ggccgttttc gcgttgcgcc ggcccggttc gcggcggaca tcacgtcggc gtttctcgaa
     3121 acgcggttgg tcgtgcgatc gaaccaaccc ccgagcttca ttccgagtct cgtcgagatc
     3181 ctctggttcg gtccgccgga agccaagcgt ctcgcgctgc ccgagctggc cgcgcgccga
     3241 ttcggcccgg cgatgttgag cttcatcgac gcggcctacg agcacccgga gctgacgggc
     3301 ccggtgatca gcgcactcgg ccggttgcgc gacgacaggg cgcgtcatta cctcggtgag
     3361 atcctgcttc gagccgattc gacctacacg cgtccggcgg ccgaggcgct ggcgcggatc
     3421 ggtggccgcg cgatcgacgt gctgcgcgag gcgtcggcgt cccccgacga gctcgtgcgc
     3481 ggctcggcga tcgacgccct ggtgccgctg tccggtgtca cggatctgac gatcctctac
     3541 gaatacgtcg cgcagtttcc ggacgacgac ccgcgtcgca tcgagctggt gatccagcgc
     3601 gcgacccaac tcgaaacgct gctcgaggcg cggcaggacg tcgacgccgg atccgccacc
     3661 gacttctgaa actcagcggc gcgtgagctc cgcgcggatc cgcccgacgc cttcgcggat
     3721 ccgctcgggc tcggccgcga acgagaccct caaccacccg gcgccgcgct caccgaaggc
     3781 ctcgcccggg atgacgatca ccgcctgtcg ttcgaggatg cggcgggcca gcgcgaccga
     3841 atcaccgtgc gcgcgaacat cgacgaagta atagaacgcg ccgtccgggc gatggaactc
     3901 gagtcccgcg atgccttgca gctcgtcggc catcagctcg cgacgcgacg ccaagagctc
     3961 cgcgtggcgc gcgcgcgcac ggcggccggc cggggtgaac gcggcgaggg ccgcgccctg
     4021 cgagatcgtc gacgcgcagg tgagcaagta ctggtgcgcc gccgtcacgc gcgcgacgac
     4081 ggccggcgga ccgacgaccc aaccgactcg ccagcccgtc atgctcatcc ccttcgacag
     4141 tccggcaacg accaggccgc cgccgttcga gtagctcgcc ggcgaggcaa acgctcggtc
     4201 ccagacgaag ccggcgtaga cctcgtccga cagccaggcg acgccgcgag cggccagcgc
     4261 gcgggtcagc gcctcgaccg cggcgggctc ggcgacggcg ccggtcggat tggacggcga
     4321 gcacagcacg acgacccgcg tcctctcccc gacccggtcg agcacggcag cggcgtcgag
     4381 tcggaaccgg cgcgatgggt cgagcggata gggcgtcgcc gtcgctccca gcatgcgtgc
     4441 gacgacggga tacgccggat aacccgggtc ggggtagagc agttcgtccc cggcatcgag
     4501 caacacgagg cagctcgcga acatcgcttc ctgcgagccg acggtgatgc agaccgactc
     4561 gggaccgtcg gcgaagccgg ggtactcccg ggcgaccgcc gcccgcagga gcgggtcgcc
     4621 ggccgtcgac gtgtagccgg tgcgcccctc ggcgatcgtc gcgatcccgg cgagcgcgca
     4681 gggatcgggg gtcggcagac ccggctcccc caggccgagg ttgatcgctc ccggcggggc
     4741 ggcatcgaag atgcggcgga tcagtgtgcg atcgataccg tccatccgtg ccgaaatccg
     4801 cagcgtcaac gcaacgcccc ctcgagcgcg agcgccgcgt cggtccgtgc gtcgcggtac
     4861 ttgacgatca ccggggcgtg gagcttcagc ccgtgctccg cggcgaactc ccccgtcgcc
     4921 ggtcgcgcgc cggggacgac gaccgcaccg tcggggacga cgagcgaccc gtcttcgccg
     4981 gcgcgatgga ccgcgcgccg tggcaggtcg tacagcgcca ccgcccggct gagcacgacc
     5041 cccggggcga gcaccgcccg gcggccgatg cgcacgcctt catagatgcc gcagcccccg
     5101 ccgagaaacg cgccgtcctc gacgatcacc ggaagcgtgc cgatcggctc gagcaccccg
     5161 ccgagctgga ccgcggccga caggtggacg tcgcggccga cctgggcgca cgagccgacg
     5221 agcacgtggc tgtcgatgag cgagccggcg ccgacgaacg ccccgacgtt gacgtaggac
     5281 gggggcatga tcaccacccc gtccgcgacg tgcgcccccc ggcgcaccga cgagcctccg
     5341 ggcacgaccc ggacctcgct gccgtcgcgc gggcgccaca gcggcagcgt gtcgcggtcg
     5401 tgaaagacca ggccgtcggc cgcgccggag cggacgttgc gaccgatgcg gaatgcgagc
     5461 aggatgccct gcttgaccca ggggttgacg acccacgacc cgtcgccggg cgaggcggcg
     5521 cgcacgcgtc cctcctcgag cgcgtcgaac agccgctcga tcaccgcccg cgccgcggcg
     5581 gtgtcgagcc gttcggcctc gatcagcgat tcgatctcgc cccgcaggcc gaggctcatc
     5641 gggcgccggc cgtcacggcg gcccgccgca acgcctcggt gacggcgtcg acggtggccg
     5701 cctgtggagg gccgagcgga gcacgcagca ccagctcgca gaaaccgagc cgatgcatcg
     5761 ccgttttcac cggcaccggg ttgctctcga cgaagttggc gcgcatcaac ggcagcaggc
     5821 gatagtgcag ctccctcgcc ggctgcagct cgccggcacg ggcgtgcgcc acgagtcgcg
     5881 ccatctccgc cggggcctcg ttggcgacga ccgagatcac gccttccgcg cccagcgcga
     5941 cggtcggcag cgcgagcggg tcgtcgccgg aaaggacgac gaagccgggt ggccgggcgg
     6001 cgaggatccg ggcgatttgc tcgaggtcgg ccgacgcttc cttgacgccg atcacgcccg
     6061 ggatctcggc cagcgcgagc gtcagctcga ctcccaggtt ctgccccgtc cgacccggca
     6121 cgttgtagac gatcaccggc agcgcggtcg cgccggcgac ggcctcgtag tgcgcgagca
     6181 tcccgctcgc gttcggtttg ttgtagtacg gcgtgacgac gagcaacgca tccgcgccgg
     6241 ccgcggcgca ccgcttcgcg ccgtcgaccg tcgtccgggt gttgttcgtg ccgcacccgg
     6301 cgatgatcgg cacgcgaccg gcagccgcgt cgacggtcgc ggcgacgact tcggcttttt
     6361 cgtcctcgcc cagcgttgcg gcctccccgg tcgtgccgca cggcacgagg gcgtcgatcc
     6421 cctgctcgac ctgccaggcg accagacggc agagcgccgc gcggtcgatc ttccccgcgc
     6481 cgtcgaacgg ggtgatcaac gcggtcatcg cgccggaaag tgtgcttgcc aacccgtggt
     6541 tcgccatcat ccaggcctcc cgtgccgagc gtcgcgtcaa cgacgcgcgg cgcgcacgag
     6601 ctcgtccacc acctcgtcga atccgtgaag tccggttttg cccgcgatcc attcgccggc
     6661 cagcaccgcc ccggccgcga acccgctccg tccccgcgcg cggtgcgtca gctcgatccg
     6721 atcgtggtcg ctgtcgaacc cgacgacatg ggttccgggc tcgaattcta cccggaggct
     6781 cgcgacgtgc aatgcgccgt cgggcagccg ccgttcggga ttgccctcga cgaccgaccg
     6841 caggcgggga tcgcgctcgc gcaggatcct cgccagcgct cttgccgttc cgctcggggc
     6901 gtcgagcttc cccgtgtggt gcatctcgaa gatcgaggga cggtggctgt cggcggccgc
     6961 gaacaacccc gccgcgtggc cgaggatgcg gcagaacagc tgcatgccga gcgagaagtt
     7021 cgcggcgacg atcgcacccg ccgctcggcc ggcgatctcg cgatggaacg catcgtccgg
     7081 gtcccagccc gtcgtcccac agacgaccgc gacgccggca ccggtcagcg cgagcacgtt
     7141 ccgtcgagcg gacgacggct cggtgaactc gaacgccacc tcgggcacgc ccatcgcggt
     7201 ccgatcgacc cgtccgggag ccaaccgcgc ggcgtcgacc gagcggagcc gaccgacgca
     7261 ggtgtggccg cgacccgctg cttccgcctc gatcgcccgc cccatccggc ccgcgcccac
     7321 cagcaggtaa cgcatcacgc ggcgccctcg aggtagcggc ggtgcaggtc gcgcaccgcg
     7381 ccgatcagac gatcttcctc gacgaccgcc gcgagactgg cacccgagct gccgagggcg
     7441 agcagctccg gttgccaggc ggccaaggca cacacggcgc ggctcctctc ggcgccgacc
     7501 ctcaacgccg acccgacgat gcagatcacc gcgcgacgtg gctcgacccg gacccggccc
     7561 aaccgctcga gcgccgccac gagggggccg tggtcgccgc tcgacgagcc gacgacgtgc
     7621 accgcaagct cggaagcgac gacgagctcg gggacgaccg agcattcgcc gaacaccgcg
     7681 agcaccgcgg cggcgaaggc ggcgtccggc cgcatcttgc ggttgacgac gctcaccgtc
     7741 cgcacgccgg cgcgactggc gaccgagacg actcgttcgg gctccgtgct atcgacctcg
     7801 ccgacgacga cggtgccggg cgccgccggg cgaagcgagt tgcggatacg aagcgggatt
     7861 ccgcgccgga ccgccggggc caggcagtcc gggtgcagca cgcgtgcacc gtagaacgcg
     7921 agctcggtcg cttcggcgaa ggacagccgc tcgatcggcc gggcgtcggg gaccaagcgc
     7981 gggtcggcgg acatcatccc gtcgacgtcc gtccagatct ggatctcgtc cgcccccaag
     8041 gcggcgccca gcaccgccgc cgacgtatcg gagccgccgc ggccgagggt cgtggtctcg
     8101 cccccgaccg tcgcgccgac gaaaccaccg agcaccggga tccgcccgcc gcggatcagc
     8161 ggctccaaca ggcggcgagc ccgctcggcg acgccggcgg cgttcggttc ggcctgtccg
     8221 aaccgatcgt cggtcacgac cacccgccga gggtcgaccc aggtcgccgg ctggcccgct
     8281 tcgaccagcg cccgggtgac gatcgtcgac gacagcgtct ctccggtcgc caggatggcg
     8341 tcggccgttc gcgggctccg ttcgccgagg atccggatcg agcgcagttt ctgtcgcaac
     8401 tggtcgaaca ggccgtcgat ctcgacggac agatggtggc gctccttggc cgtctgctcg
     8461 agaccactca tcgcccagcg gtgccggcgc tcgagatcgg cgagcaacgg ttcgaccgcg
     8521 tcgggatcgc cgccgagcgc ggctccgacc aggcgctcga gtgtatcggt cacgccggcg
     8581 agtgccgaga ccacgaccag cggagcacga tcgcgcgctc ggccgacgat ctcggcgacc
     8641 gagcggatcc gatccgcgtc cgcgaccgag gtcccgccga acttcatcac gatcacgccg
     8701 cgcccccgcg cctgggcaac agctctcgcg cggcgagcag ctcggcgttg agcagcgtgc
     8761 caccggccgc accgcgcacc gtgttgtgcg acagcacgac gaatcggagg tcgagaaccg
     8821 gacagcgtct gagacgaccg acgacgacgg tcattccgct gccttcgtcc cggtcgaggc
     8881 gcggttgcgg gcggtcgtcc tcgtcacgga cgagcagggg tcgtgccttg gccgaaggca
     8941 gtgatccggg ctcgatcgcc ccgcggaaat cgagcagcgc gccgcgtacc gcgtcgagat
     9001 cggcccccac cgccagcttg accgagaccg cctcgaggtg gccgtcgatc gtcggcacac
     9061 ggtgacagtg cgccgagacg acgagcggag ccggctcgat ccgtgccgct tcgacccgac
     9121 cgaggatctt gccgacctcc cgttccatct tctgctcttc gcccgggatg tgcggcagga
     9181 cgttgtccag cagctggagc gcggtcggtc cggcgagacc ggcgcccgag gccgcctgca
     9241 tcgtcgtcac gacgacctgc gagacaccga acgccctgtg cagcggtgcg agagcgacga
     9301 ccagaccggt cgtcgagcag ttggggttgg tcacgatgta gccaccgtcg cgccgtcgcg
     9361 gtccatcgat cagctcgagg tgggaggggt tgacctccgg gacgaccagc ggcacctcgg
     9421 catgctggcg gaacgccgag ctgttgctga cgaccgggat gccggcgccc gcgatcgccg
     9481 gttcgagctc gcgggcgatc tccgcgtcga gtgccgacaa cacgaggtcg cagtcgtcga
     9541 gctcgtcgac tcgacagcgg cgcacacgca gctgcgcgaa cgccgcgggc ggcccgccgc
     9601 cgggtagctg ccagttcgcg gcctcgtcgt acggcttgcc ggccgaccga tccgacgccg
     9661 cgagtgcacg gagctcgaac cacgggtgac ggtcgagtcg ctcgacgaga cgctgtccga
     9721 ccagcccggt cgcgccgacg ataccgacac ggtacctgtt catccgctcc acctcttggc
     9781 ccacgatagc atcggcgtcc atgttatact cgccgtttcg aagtgatgaa gaaccgatcc
     9841 gagctgcgcc ccgtgagcga accgagcgag cagaacggcg agatctccgt ttgcgggcga
     9901 ggaaaatatc gcgccccgga ggacgatttc tactgcctgc gctacggcgt ctggtacccg
     9961 tcgatcgact gcgcgtttcg gacacgctac cgcaccgcgc ccggttgtcg cgactgcgag
    10021 cagggtcggt tcaatctcaa acggcacgcc cgggcgatcg cacgccccgg tcggttccgt
    10081 tcgggctgag gtgatcgccg acctccgccc gccgccgctc gatccgcttc gcgtccgcgc
    10141 gatcgtcttc gacctcgacg gcacgttgat cgattcgtac ggggcgatcg ccgacgcgtt
    10201 gaaccacgcg cgggaccggt acgacctgcc cccgctggcg g
//
LOCUS       DASPGN010000574         5051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_343195, whole genome shotgun sequence.
ACCESSION   DASPGN010000574 DASPGN010000000
VERSION     DASPGN010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343195"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VD788_16520"
     CDS             <1..863
                     /locus_tag="VD788_16520"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HXV77917.1"
                     /translation="AAVGRRGRAVHRAPRDAFGSDERIASEWRELGYVGPPVLARAIE
                     QFDRLVELLDGLGIEVRIAEPRARLGLDSIYVRDAAVVCDDGAILCRMGKPARAAEPQ
                     ALAAALESAGCPVLGAIDGDGRLEGGDVVWLDRRTLAVGRGYRTNDEGIAQLRELLGA
                     HIDRLITVPLAHGEGPASVFHLMSMISPLDHDLALVHSAPMPIPFRETLIGYGIRLIE
                     AAAAEIGGLACNVLTVAPRHCLMLDGNPRTRKALEQAGVRVESFAGDEICLKGAGGPT
                     CLTRPLQRFV"
     gene            939..2297
                     /locus_tag="VD788_16525"
     CDS             939..2297
                     /locus_tag="VD788_16525"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXV77918.1"
                     /translation="MSDKPTGAVSSDRDEPVPIDRDRVRAIVERSGVDVRRASIREMN
                     KLVNTIEQQLEIRFVRMEFGIPGLPLDPVAIDAESQALRERHVGNVYAPFDGVPELKR
                     EASRFVKLFMDLDIPPACCIPTVGAMEGCFASLALAAQIKRGRNKIICLDPGFPVNKL
                     QIRFLGLERESIDFYDHRGQVLVDAVDRLASRGDVAAVLWSSPNNPSWIVLRETELAG
                     LGAVCDRHDILAIEDLAYFGMDVRRDYTVPGQPPFQPTVLRHTHNGICIISSSKMFSY
                     AGQRIAIAILSPELMDKRAPDMVERFGTANVGHAFIHGVLYPIAACVAETPQYGMLSL
                     LREVNDGNRRLFWPALEYSRRARIVKKIFLDNGFRLVYDNDLGQPLADGFYFTVSHPA
                     YESGAELLMELLHYGVSAITLETTGSTRTEGLRACVSLIGDEQFATLRARLERFRRDH
                     PV"
     gene            complement(2362..3267)
                     /locus_tag="VD788_16530"
     CDS             complement(2362..3267)
                     /locus_tag="VD788_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77919.1"
                     /translation="MITKSTSGWIALAAVLTASTFVAGCGDNVADNGADPIENPEIAA
                     PTGVDPSAEEETSESDEWAMRQQQLDERMRALEQREAEIAQRERQAARQREAAELRVS
                     EPPVVRDPEPAVREVEVTLPASTALAVEFTSGLSSDESVAGDPVRVVVVRDVVQDGRV
                     AVPAGSQVSGQVAEVKSGRKIGGRSRVVLEFDTLELPEGDRFPVRSTIEYAGKSQTGK
                     DAATIGGSTAGGAILGRVLGGDDKDKSTAIGAVVGAAVGTAVAAKNGTDSVVIQTGDV
                     AELLLYEPVRLNWRETVPATDWARN"
     gene            3518..4570
                     /locus_tag="VD788_16535"
     CDS             3518..4570
                     /locus_tag="VD788_16535"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXV77920.1"
                     /translation="MSNPLARTVSLAPLVVLALLVGCSSASSNADATASEAAAGTAEV
                     AVKLGDETITLAEVDEKSRTISLQPYQALYDQRKMAIDQLVAERLIEKEAKSRGLTAE
                     ELVEAEISAKMPELTEVDVERFYNQNTPSMGGRQLDDNLRAQIRQFLDQQRVMVARQN
                     FLVALRADTDVAILLEPPRVELTVKPDEPSDGPVDAPVTIVEYSDFQCPYCARVGPTL
                     DQIKQAYGDRVRIVFRDFPLPNHPQAIPAAQAAKCANEQGKFWDYHDKLFASQRELGP
                     DKYKQFAVELGLDSEKFDACVDSNKYAQQVQLIATGGQQAGVTGTPAFFVNGRFVSGA
                     KPFEAFKEIIDEELAN"
     gene            complement(4578..>5051)
                     /locus_tag="VD788_16540"
     CDS             complement(4578..>5051)
                     /locus_tag="VD788_16540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77921.1"
                     /translation="ALAEDERGFQSDDRRHVVKLNATSIMPWGLRLGGALSWRSGLPY
                     SVLAEELSFDSLPLPYGNSGTGGQARPRQLYVDGQRNTERNPSYWNLDAKLVKELLVG
                     RTVNLQLSAEVFNLFDDRTYQIYNPALGLGRRLNGENEAFRRTGRSWQLGVRLTF"
BASE COUNT          795 a         1752 c         1686 g          818 t
ORIGIN      
        1 acgccgcggt gggtcggcgc gggcgggccg tccaccgggc cccgcgcgac gcgttcggca
       61 gcgacgagcg gatcgcgtcc gagtggcgcg agctgggcta tgtcgggccg ccggtgctcg
      121 ctcgcgcgat cgagcagttc gatcggttgg tcgagctgct cgacggcctc gggatcgaag
      181 tgcggatcgc cgagccacgc gcgcggctcg ggctcgattc gatttacgtc cgcgacgcgg
      241 cggtcgtctg tgacgacggc gccatcctct gccggatggg caagccggcg cgcgccgccg
      301 agccgcaggc gctggccgcc gcgctcgaat cggccggctg tccggtgctg ggagcgatcg
      361 acggcgacgg ccggctcgaa ggcggcgacg tcgtttggtt ggaccgccgg acgctggccg
      421 tcggccgcgg ctatcgcacc aacgacgagg ggatcgccca gttgcgcgag ttgctcggcg
      481 cgcacatcga ccgtctgatc acggtgccgc tcgcgcacgg cgaggggccc gcgagcgtat
      541 tccacctgat gtcgatgatc agcccgctcg accacgactt ggccctggtc cactcggcgc
      601 cgatgccgat tccgtttcgc gagacattga tcgggtacgg gatccgcctg atcgaggccg
      661 ccgccgccga gatcggcggg ctcgcgtgca acgtgttgac cgtggccccg cggcactgcc
      721 tgatgctcga cggcaaccca cgaacgcgga aggcgctcga gcaggcgggg gtccgggtcg
      781 agtcgtttgc cggggacgag atctgtctca agggcgcggg cgggccgacc tgtctcaccc
      841 ggccgttgca gcgattcgtc tgagagatct cgatcgcgga ccgaggcggt tgccaagcgg
      901 atttgatgcg gccgcgtccg atccgctatc tttgcccggt gagcgataaa ccgacgggag
      961 cggtgtcgag cgatcgcgac gaacccgtgc cgatcgatcg cgaccgggtg cgagcgatcg
     1021 tcgaacggtc gggcgtcgac gtacgtcgag cctcgatccg cgagatgaac aagctggtca
     1081 acacgatcga gcagcagctc gagatccgct tcgtgcggat ggagttcggc attcccggtc
     1141 tgccgctcga tccggtcgcg atcgacgcgg aaagccaggc cctgcgcgag cgtcacgtcg
     1201 gcaacgtcta cgcgccgttc gacggcgtgc ccgagctcaa gcgcgaagcg tcccggttcg
     1261 tcaagctgtt catggatctc gacattccgc cggcgtgctg catcccgacc gtgggagcga
     1321 tggaaggctg tttcgcctcg ctcgcgctcg ccgcgcagat caagcgcggc cgcaacaaga
     1381 tcatctgtct cgaccccggt ttcccggtca acaagctgca gatccgcttc ctcgggctcg
     1441 agcgcgagag catcgacttc tacgaccacc gcgggcaggt gctggtcgat gcggtcgacc
     1501 gcctcgcctc gcgcggcgac gtcgcggcgg tgctgtggtc gagcccgaac aaccccagtt
     1561 ggatcgtgct ccgcgagacc gagctcgcag ggctcggcgc ggtctgcgac cgtcacgaca
     1621 tcctggcgat cgaggatctg gcctacttcg gcatggacgt gcgccgcgac tacaccgtcc
     1681 ccggccaacc gccgttccag ccgaccgtgc tgcgccacac gcacaacgga atctgcatca
     1741 tcagcagctc gaagatgttc agctacgccg ggcagcggat cgcgatcgcg atcctctcac
     1801 ccgagctgat ggacaagcgg gcacccgaca tggtcgaacg cttcggcacg gcgaacgtcg
     1861 gtcacgcctt catccacggc gtgctctacc cgatcgccgc ctgtgtcgcg gaaacccccc
     1921 agtacggcat gctgagcctg ctgcgcgagg tcaacgacgg caaccgccgt ctgttctggc
     1981 cggcgctcga gtactcgcgc cgggcacgca tcgtcaagaa gatcttcctc gacaacggct
     2041 tccgtctggt ctacgacaac gacctgggac agccgctggc cgacggcttc tacttcaccg
     2101 tctcgcaccc ggcctacgag tcgggagccg agctgctgat ggagctgctg cactacggcg
     2161 tcagcgcgat cacgctcgag acgaccggaa gcacgcggac ggaagggctg cgggcttgcg
     2221 tctcgctgat cggcgacgag cagttcgcga cgctgcgcgc gcgcctggaa cggtttcggc
     2281 gcgatcaccc ggtctgatcg gagcgggagc cgagtcgcag caggcccggc tcccgtccac
     2341 gcttcggccg cgattgtcgt ttcagttccg ggcccagtcg gtggcgggga ccgtctcgcg
     2401 ccagttcagc cgcaccggtt cgtacagcaa cagctcggct acgtcgccgg tttggatcac
     2461 caccgaatcg gttccgttct tcgcggccac cgccgtgccg accgcggcac cgacgacggc
     2521 gccgatcgcc gtgctcttgt ccttgtcgtc gccgccgagc acccgaccga ggatcgcgcc
     2581 gccggcggtc gagccgccga tcgtcgcggc gtccttgccg gtctggctct tgccggcgta
     2641 ctcgatcgtc gagcgaaccg ggaagcggtc gccttccggt agctcgagcg tgtcgaactc
     2701 gagcacgacg cgtgatcgcc cgccgatctt ccggccggac ttgacctcgg ccacctggcc
     2761 gctcacctgg ctaccggcgg ggaccgcgac acgtccgtcc tggaccacgt cgcgcacgac
     2821 gaccacccgc accggatcgc cggcgacact ttcgtcactc gacagtcccg aggtgaactc
     2881 gaccgcgaga gccgtcgagg ccggcaacgt cacctcgacc tcgcgcacgg cgggttccgg
     2941 gtcgcgcacc accggcggtt cggacacgcg gagctcggcc gcctcgcgct gccgcgccgc
     3001 ctggcgctcg cgctgggcga tctcggcctc gcgctgctcg agcgcccgca tccgctcgtc
     3061 gagctgttgc tggcgcatgg cccattcgtc gctttcgctc gtctcttcct ccgcactcgg
     3121 gtcgacaccc gtcggcgcgg cgatctccgg gttctcgatc gggtccgcgc cgttgtcggc
     3181 cacgttgtcg ccgcaaccgg cgacgaaggt cgaggccgtg agcacggccg cgagcgcgat
     3241 ccaaccgctt gtcgatttcg taatcatcgg ttgccctcct tgcgaggtgt cggacaccag
     3301 acggagcagc aagccccgta ccaagccgaa accggcggga atctcccttt ttccgcggag
     3361 gcgggccccg tcgcgctgtg gattccggac gccggccgtc acccgccggc tacacgacgg
     3421 gcatgcttct cgttcatctg gtagccttgt cgggtcatcc cggcccgcgc cgatgcgcgg
     3481 agtcgatgaa accgcgcaca tttccgagga acgttccatg tccaacccgc tcgcaagaac
     3541 cgtaagtctc gcaccgctcg tcgtgctcgc cctgctcgtc ggctgttcga gcgcatcgtc
     3601 gaacgccgac gccaccgcgt ccgaggccgc ggccggcacg gcggaggtcg ccgtcaagct
     3661 cggcgacgag acgatcacgc tggccgaggt cgacgagaag tcgcgcacga tcagtctcca
     3721 gccgtaccag gcgctctacg accagcgcaa gatggcgatc gaccagttgg tcgccgaacg
     3781 gctgatcgag aaggaagcga agtcccgcgg gctcacggcc gaggagctgg tcgaggccga
     3841 gatctcggcg aagatgcccg agctgaccga ggtggacgtc gagcgcttct acaaccagaa
     3901 cacgccatcg atgggtggac ggcagctcga cgacaacctg cgggcgcaga tccggcagtt
     3961 cctcgaccag cagcgcgtca tggtcgcgcg gcagaacttc ctcgtcgcgc tgagggccga
     4021 taccgacgtt gcgatcctgc tcgagccgcc gcgggtcgag ttgacggtca agccagacga
     4081 gccgtccgac ggtccggtgg acgcgccggt cacgatcgtc gagtactccg acttccaatg
     4141 tccgtactgc gcgcgcgtcg gtccgacgct ggaccagatc aagcaggcct acggcgaccg
     4201 ggtgcggatc gtcttccgcg atttcccgct gccgaaccac ccgcaagcca ttccggccgc
     4261 gcaagccgcc aagtgcgcca acgagcaggg caagttctgg gactatcacg acaagctgtt
     4321 cgcatcgcag cgcgagctgg ggcccgacaa gtacaagcag ttcgccgtcg agctcgggct
     4381 cgactcggag aagttcgacg cctgcgtcga ctcgaacaag tacgcgcagc aggttcagct
     4441 gatcgcaacg ggcggccagc aagcgggcgt gaccgggaca ccggccttct tcgtcaacgg
     4501 tcgcttcgtc tcgggcgcca agccgttcga ggcgttcaag gaaatcatcg acgaggagct
     4561 ggccaactga ctctcgtcta gaacgtcaac cgtacgccga gctgccaact ccggccggtg
     4621 cgccggaagg cctcgttctc cccgttgagt cgtcgtccga ggccgagcgc ggggttgtag
     4681 atctggtaag tccggtcgtc gaacaggttg aacacctccg ccgacaattg caggttgacg
     4741 gtgcgcccga cgagcagctc cttgaccagc ttggcgtcga ggttccagta cgacgggttg
     4801 cgctcggtat tgcgttggcc gtcgacgtag agctgacgcg gccgggcttg accaccggtg
     4861 cccgaattgc cgtagggcag cggcaacgaa tcgaacgaca gctcctcggc cagcaccgag
     4921 tagggcagac cggagcgcca gctcagcgcg ccgccgagac gtagacccca gggcatgatc
     4981 gacgtcgcgt tcagcttgac gacgtggcgc cggtcgtcgg actggaaccc gcgctcgtcc
     5041 tcggccagcg c
//
LOCUS       DASPGN010000575         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_810613, whole genome shotgun sequence.
ACCESSION   DASPGN010000575 DASPGN010000000
VERSION     DASPGN010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810613"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..513)
                     /gene="metG"
                     /locus_tag="VD788_16545"
     CDS             complement(136..513)
                     /gene="metG"
                     /locus_tag="VD788_16545"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HXV77922.1"
                     /translation="MNDTPRPNDERPPGPPGDDSITIDDFLRIELRVARVLAAEAVPN
                     TDKLLRLEVDLGDERRQLVAGVALAYAPEALIGKQIIVVANLKAARIRGVESRGMLLA
                     ADAGGRPIVATFDEPVPPGTRVR"
     gene            complement(510..1745)
                     /gene="larC"
                     /locus_tag="VD788_16550"
     CDS             complement(510..1745)
                     /gene="larC"
                     /locus_tag="VD788_16550"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HXV77923.1"
                     /translation="MRVLFVDGSSGASGDMLLGALVDLGLPVAALRRALAGLPVAGGT
                     QSSRRLVRCGLAGRKVDVRVRGRQPHRGLHALERIVERGGLSPSVRDRAFAVFRRLVE
                     AEAEVHGLAVDRTHLHEAGGTDAVIDVVGVCWGLERLGVERLVVSPLTTGFGTVVCQH
                     GTYPVPAPATLLLMRGLPIEAGGLAAERLTPTGAALLTTLADGFGPMPAMRPSRVGYG
                     AGDREFDSDPNYLRAVLGDLVEPTPYGASGSGTEVEVIEATVDDMTPQGVAHAAERLR
                     EAGALDVFTTAVVMKKGRSGHLLTVLCRPERFDAIARCVIEQTTSLGLRHRRERRIEL
                     GREIVAVRTAWGVVRVKRAWTADGAPRVMAEYDDCARLARERGVTLAEVQRAAIEAAR
                     RGAAGQRKRRNDQPRRRAK"
     gene            complement(1745..>2067)
                     /locus_tag="VD788_16555"
     CDS             complement(1745..>2067)
                     /locus_tag="VD788_16555"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HXV77924.1"
                     /translation="PPPRCDCGELLRPAVVWFGEELPEDVVLRAESAVRDASVVLVVG
                     TSSLVYPAAALPQLARAAGAYVVEVNPEATPLTSQVDERLAGPAGEVVPALALAAGVP
                     EDDG"
BASE COUNT          309 a          793 c          691 g          274 t
ORIGIN      
        1 gcagcgcggg aggcaacaac aacagctgat ccggatcgta cgggcggaag gtcttgtcca
       61 tgccccgatt attccagacc cgccaatccg caggaactgg aaactacaat cgaatccgat
      121 tactcggaca ggctcctagc gcaccctcgt tccgggtggc acgggctcgt cgaacgtcgc
      181 cacgatcggc cggccacccg cgtccgccgc cagcagcatc ccgcggctct cgacgcctcg
      241 aattcgcgcc gccttcagat tggcgacgac gatgatctgt ttgccgatca acgcctccgg
      301 cgcgtaggcc agcgccacgc cggcgacgag ctgccgccgt tcgtcgccga ggtccacctc
      361 gagccgcagg agcttgtcgg tgttcggcac ggcttcggcg gccaacacgc gcgccacgcg
      421 tagctcgatg cggagaaaat cgtcgatcgt gatcgaatcg tcgccgggcg ggcccggcgg
      481 tcgttcgtcg ttcggtcgcg gtgtgtcgtt cacttcgctc tcctgcgtgg ttggtcgttc
      541 ctccgcttcc tctggccggc cgccccgcgc cgcgcggcct cgattgcggc gcgctgcacc
      601 tcggccagcg tcacgccgcg ttcgcgcgcc agccgggcgc aatcgtcgta ctcggccatc
      661 acgcgcgggg ccccgtccgc cgtccaagcc cgcttgacgc gcaccacgcc ccacgcggtg
      721 cggacggcga cgatctcgcg ccccagctcg atgcgccgtt cacgtcggtg gcgcagcccg
      781 agactcgtcg tctgctcgat cacgcagcgg gcgatcgcgt cgaagcgctc cgggcggcac
      841 agcacggtca gcagatgacc gctgcggccc ttcttcatca ccaccgccgt cgtgaacacg
      901 tcgagcgcgc cggcttcgcg cagccgctcc gccgcgtggg ccacaccctg cggcgtcatg
      961 tcgtcgacgg tggcctcgat cacctcgacc tcggtgccgg aaccgctcgc cccgtaaggc
     1021 gtcggctcga cgagatcgcc gaggacggcg cgcaggtagt tcgggtcgga gtcgaactcg
     1081 cggtcgccgg caccatagcc gacgcggctc ggacgcatcg cgggcatcgg accgaagccg
     1141 tccgccagcg tcgtcaacaa ggcggccccg gtcggggtca gccgctccgc ggcaagcccg
     1201 ccggcctcga tcggaagacc ccgcatcaac agcagcgtcg ccggcgccgg caccgggtag
     1261 gtcccgtgct ggcagacgac cgtaccgaat ccggtcgtga gcggcgagac gacgagccgt
     1321 tcgacgccca gccgctcgag cccccagcag acgccgacga cgtcgatcac cgcgtcggtg
     1381 cccccggcct cgtgcaggtg ggtccggtcg accgccaggc catgcacttc cgcttcggcc
     1441 tcgaccagcc tgcggaagac cgcgaaagcg cggtcgcgca ccgacgggga caaccctccg
     1501 cgctcgacga tccgctcgag cgcgtgcagg ccgcgatgcg gttgccggcc gcggacgcgg
     1561 acgtcgacct tgcgtccggc gagcccgcag cggacgagac gccggctgga ctgcgtcccg
     1621 ccggcgacgg gtagcccggc gagcgcccgg cgcagcgcgg cgaccggcag accgaggtcg
     1681 acgagcgccc cgagcaacat gtcgcccgac gcaccgctcg agccatcgac gaacagcacg
     1741 cgcatcagcc gtcgtcctcc ggcaccccgg ccgcgagcgc gagcgccggc acgacctcgc
     1801 ccgccgggcc ggcgagacgt tcgtccacct gcgaggtcaa cggtgtggct tcgggattga
     1861 cctcgacgac gtacgcgccg gccgcacgtg cgagctgcgg gagcgcggcc gccgggtaga
     1921 ccagcgacga cgtgccgacg acgagcacca ccgacgcgtc acgcacggcg ctctcggccc
     1981 gcagcaccac gtcctcgggc aactcttcac cgaaccagac gaccgccggt cgaagcagct
     2041 cgccgcaatc gcagcggggc ggcggtt
//
LOCUS       DASPGN010000576        12214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_460016, whole genome shotgun sequence.
ACCESSION   DASPGN010000576 DASPGN010000000
VERSION     DASPGN010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12214
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460016"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VD788_16560"
     CDS             <1..1080
                     /locus_tag="VD788_16560"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXV77925.1"
                     /translation="EGAALVVRPVTGAIVAMVGGRDYGRSQFNRATQARRQPGSCFKP
                     IVFAAGLEAAAEGRPDGLTPATLLEDQPLELVSGGKTWSPANYDREFRGIVTVRHALE
                     RSLNVPTVRAAQAVGLDRVVTTARRLGIASPMQPLPSLALGTAEVSPLELATAYAAFA
                     AGGTRTTPWIIRAVLDGDGARMEAQTVERGPAVSAQTAFLLTDMLRGVFERGTARSAT
                     DYGFRGEAAGKTGTTDDTRDSWFVGYDDELFGLVWLGYDDNSRTGLTGATGALPIWVE
                     LMSRTAGAHRLSPEVPEGVVSRRIDAQTGELARSGCDEVIREYFIRGTEPLSDCHLHE
                     GRFKRWFERILGRGERERGGKRRSG"
     gene            1122..1616
                     /locus_tag="VD788_16565"
     CDS             1122..1616
                     /locus_tag="VD788_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXV77926.1"
                     /translation="MRSFESMLRRRGEAGVTYVEMLVAMAVLAILVSAAVPLARWDQK
                     RRAEVRLKTTLRVMRAAIDKYKEYADQGLIILEDVDQMGYPRDLDELVEGVEVGAGDS
                     PDAKKIKFLQRFPVDPMTGEAEWGKRSYQDDWDSDSWGGENVYDVYSLSDRRALDGTY
                     YRDW"
     gene            complement(1626..1712)
                     /locus_tag="VD788_16570"
     tRNA            complement(1626..1712)
                     /locus_tag="VD788_16570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1676..1678),aa:Leu,seq:gag)
     gene            complement(1768..2208)
                     /gene="secG"
                     /locus_tag="VD788_16575"
     CDS             complement(1768..2208)
                     /gene="secG"
                     /locus_tag="VD788_16575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXV77927.1"
                     /translation="MLGVLLTIVHVIVAVFLIMVVLLQTGKKADLAGAFGGGGSQTAF
                     GTRGAATLLSKLTTVAAVSFMLTSLALSLIASNAVSQKGGSVLPEDTPTSRSEQVPPP
                     STPGSPVDQPAGEDAGSPPADNPGGAPPTSDDEPPPVEGPATPD"
     gene            complement(2296..3612)
                     /locus_tag="VD788_16580"
     CDS             complement(2296..3612)
                     /locus_tag="VD788_16580"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXV77928.1"
                     /translation="MMVSSRANEIRELVRQLGSRDRQRVNAARARLSVLGSRAVEDLI
                     AALDGDDNLVRSRAMPLLALTRDSRAREPLMAMLLDRNARIRQIASRSLGRFPGEVTG
                     RALDRVLDRDSSVGVRKAVVQSLIEHYRAGHEGAIRRPLGLLVDVCEAKHVRLSAIAL
                     VEAIPRGQRAALLARLENDPEPTIRRRTAELRAADDESAAETGSGRLLAQIADLAASD
                     YVVWNAAVQRLGDSGPEVVTPLLAEMGRRQTDAEYCTRVGMVLKALGPRRARAIVDAL
                     DREEAPLPLQVVVEVVGAFADKSMVYRLKDVIERISGDPDRYRRVRAKAHLELARVGS
                     RVGIVDLRDALASEQFELEMVAAVELIGKPDELPLLLRAYERETTFVAPRIARAVRQI
                     LKRERIRRNSRALQTLSPAQRRLLDEIVESRAAERRSRPRSEGATL"
     gene            3738..4253
                     /locus_tag="VD788_16585"
     CDS             3738..4253
                     /locus_tag="VD788_16585"
                     /inference="COORDINATES: protein motif:HMM:NF019934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HXV77929.1"
                     /translation="MIVSCIALSLSLGGCNKAERLYAEAHEHIERSEFAQAVALYDEI
                     ISTYPDSETAARASREVELYRGLATAVDFYPERRIYDQMIETARAIYKYENRRGRWPE
                     SLDRLTPDYLERPPIDPWGRELLYGVKPQRRGYVLGCYGADGRLGGDGAARDWFIEDG
                     DFVRKPSKALS"
     gene            4250..4717
                     /locus_tag="VD788_16590"
     CDS             4250..4717
                     /locus_tag="VD788_16590"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HXV77930.1"
                     /translation="MRRSVVLAALALLGGPLSVAGESGSDYSWAVAVLPSGREFKLEI
                     ADDPASRWRGYRYRERLPADEGMLFIFPVEQLQSFEMRDCLVALDIIFLDGDMRVVEI
                     AHDQQPCPAGGSCPPIQPMQQARFVLEIAGGSARREGLEPGDRVSVLAEPPIP"
     gene            4714..6069
                     /locus_tag="VD788_16595"
     CDS             4714..6069
                     /locus_tag="VD788_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77931.1"
                     /translation="MTSLVLWLLLFQSESPPGEPPADRAGAPIAAESVEIKGADLSLL
                     TSVREIAQRVEQQREQKFRSPPIAVRTPDHMREVAATIRALNVLERERLEARGRAWED
                     LGLGQPSSPERLYLLLAGDLAGIGFDPQGNRLLVAPEVLTDKDFSPQGPDDESSATLL
                     MMTGVRVDEPLASHLLMHVRQRERRGADSLAETTDELLASSAWAEGEANLVAIRYLFA
                     GMGLADAVLDPALSPADLLDGALVPPALANGGLGDSERALVRFVYDEGFFAAKTAFGA
                     GGWEALDRAMAARTTTRDLIHPGREAAAPVRVVVERPPQADGLERVDTDTLGQQAIVT
                     LVSTVTGKDNLGLQAGDGWVGDSVVRWEDPQHEPRDGVTVWCTRLRDAQAAADFDYAV
                     SRTLQARFPGRPPIEIDGDRVIQTADRVVRLRRVGVDVTLQVSARRFDELPAGGSRAE
                     R"
     gene            6190..7890
                     /locus_tag="VD788_16600"
     CDS             6190..7890
                     /locus_tag="VD788_16600"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXV77932.1"
                     /translation="MNPFGAQLLNVITYLPAVGAVLILLLFRKDQNRLVVRFATVVAG
                     SGFLVSLPLWFGWRDAAPDAWGFRFVQQADWIDSLGVRYIVGVDGISMLLVLLTTLLG
                     FVAILSAWNAVRVRVREFYAAMLLLQTGMIGVFVSLDFFLFYVFWEVMLVPMYFIIGV
                     WGGSRKIYAAVKFFIYTLLGSLLMLIGIVAVYLYHGQQTGMYTFDIRYLQAMGMWADW
                     FELQYWVWLAFFVGFAIKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRF
                     SLPMMPEATRHFLPWALGLSIVGVIYGAMVALMQKDWKKLVAYSSVSHLGLTMVGVFA
                     LNHQGLTGGVLQMINHGLSTGALFLIVGLVYERRHTREIAEYGGLWRVMPVFAVLFMI
                     AMLSSIGMPLIPGNGFVGEFTVLIGVFQLPQKIWAVLAASGIVLGAVYMLWLYQRTIF
                     GPLDRDENRKLLDLDLREIATLVPLTLIAFWIGVYPKPFFNVLDEPVRRLVQQVEGQY
                     RYPPEVVSLRPESFEERDARLASIPGPAGKRLTSTADRRPIWPRRHRRRHRLRSARQT
                     "
     gene            complement(7881..9143)
                     /locus_tag="VD788_16605"
                     /pseudo
     CDS             complement(7881..9143)
                     /locus_tag="VD788_16605"
                     /inference="COORDINATES: protein motif:HMM:NF009310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
     gene            complement(9156..9581)
                     /locus_tag="VD788_16610"
     CDS             complement(9156..9581)
                     /locus_tag="VD788_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77933.1"
                     /translation="MIASLPPAAILLLGAWLVPLLRGRLRAAYLLALPTLGLLQLVSM
                     FRLEPGSYLQLSFFDYTLTLVRVDRLSLLFGLIFSIVLLLSVIYALHVKDAVQQVAGL
                     VYAGAGIAAVFAGDLITLFVYWELTAIASVFLVWASRNE"
     gene            complement(9571..9834)
                     /locus_tag="VD788_16615"
     CDS             complement(9571..9834)
                     /locus_tag="VD788_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77934.1"
                     /translation="MMKRPGDRTPDWLDSPRNVTRLFYALLAVCLATVAIDPWFPAEH
                     PHYVYERWIGFHGVFGFVVFVLIVLAGKQLRRLVGRKEDYYDR"
     gene            complement(9845..11296)
                     /locus_tag="VD788_16620"
     CDS             complement(9845..11296)
                     /locus_tag="VD788_16620"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXV77935.1"
                     /translation="MSPGGLIALAILLPAAGALTAAVAHRHRDLREATTLVIGGALVL
                     VVVRIALTGSASLELVQLMPGIALAFDVEPLGLLFALVASTLWVVTTAYSIGYMRGHD
                     EDHQSRFYACFALSISATMGIAFARNLLTLFVFYELLTLSTYPLVIHHRTEGALRAGR
                     IYLGVLLSTSIGLLLLAVIWTWCYTGTLEFRPGGILDGRVDPRTAAILLGLYAFGIGK
                     AALMPFHRWLPAAMVAPTPVSALLHAVAVVKAGVFSILKVVVYVFGIDFLANAAASVW
                     LMHVAALTIVLASLIALTKDNLKARLAYSTISQLSYIVLGAALANRWGVIGGGMHIAT
                     HALGKITLFFCAGAIYIAADKTEISEMRGIGRVMPVTTFAFLLGSLSIIGLPPMGGSW
                     SKWLLMLGSADAQRLSLIAVLMVSSLLNIAYLMPVVIRGFFCAPPGLDGPVKIREAPL
                     FCVVPLCLTAIGCLAIFFYADEIYRLLAPVARP"
     gene            complement(11293..>12214)
                     /locus_tag="VD788_16625"
     CDS             complement(11293..>12214)
                     /locus_tag="VD788_16625"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXV77936.1"
                     /translation="MTGTLNMADMAVRLPSVSGTRTLVAAFGFLVVGIGLKMALYPLH
                     LWLPNAYTYAPSVVSSFLAATATKVSVYIMLRFAFTVFGAGFVFERLRLGWILLPLAL
                     AGAFFASTVAIFQRDVKRLLAYSSIAQVGYMVLGIGLGTLSGLTATIVHMFNHALMKG
                     ALFMAMGCIALRAGGVSIDEMRGLARRMPLTAFAWLVGGLGLIGVPLTAGFVSKWYLI
                     AAALESGLWPVAALVLLSSLLALVYVWRVVETLYFGEPREPAAGGEAPASMLGPTWVL
                     IGAVVLFGVWSAFSVGVARRAAISLLGGAP"
BASE COUNT         2047 a         4134 c         4190 g         1843 t
ORIGIN      
        1 gagggcgcgg ccctggtcgt tcggccggtg accggggcga tcgtggcgat ggtcggcggg
       61 cgtgactatg gtcgctccca gttcaaccgc gccacccagg ctcggcgcca accgggctcg
      121 tgtttcaaac cgatcgtctt cgccgccggt ctcgaggcgg cggccgaggg tcggcccgac
      181 gggctgaccc cggccacctt gctcgaggac cagccgctcg agctcgtctc ggggggcaag
      241 acgtggagtc cggccaacta cgaccgagag ttccgcggaa tcgtcacggt acggcacgcg
      301 ctcgagcgct cgctcaacgt gccgaccgtc cgtgcagccc aggccgtcgg gctcgaccgg
      361 gtcgtcacca ccgcgcgtcg actggggatc gcgtcgccga tgcagccgct gccgtcgctc
      421 gcgctcggca cggccgaggt ttccccgctc gagctcgcca cggcctacgc cgcgttcgcc
      481 gcaggcggca cgcgcacgac gccgtggatc atccgtgcgg tgctcgacgg cgacggcgcc
      541 aggatggagg cccagacggt cgagcgcggc ccggccgtca gcgcccagac ggcgttcctg
      601 ctgaccgaca tgctgcgcgg ggtgttcgag cgtggcacgg cgcgctcggc gaccgactac
      661 ggcttccgcg gtgaagccgc gggcaagacc ggtacgaccg acgacacgcg cgactcgtgg
      721 ttcgtcggct acgacgacga gctgttcggg ctggtctggc tcggttacga cgacaactcc
      781 cgcaccgggc tgaccggagc gaccggcgcg ctgccgatct gggtcgagct gatgagtcgg
      841 accgcgggag cgcaccggct gagccccgag gtgccggagg gggtggtcag tcgcagaatc
      901 gacgcccaga ccggggaatt ggcccgatcc ggctgcgacg aggtgattcg cgagtacttc
      961 atccgcggca ccgagccgct gtcggactgc cacctgcacg agggccggtt caagcgctgg
     1021 ttcgagcgga ttctcggccg aggagagcgg gaaagaggag ggaaacgccg atccggttga
     1081 cgcgcgtaga cgcccgcggg gcggccaccg taccactgcc gatgcggtcg ttcgagtcga
     1141 tgcttcggcg ccgcggcgaa gccggggtga cctacgtcga gatgctcgtc gcgatggcgg
     1201 tgctcgcgat cctggtcagc gccgccgtcc cgctcgcgcg ctgggaccag aagcgtcgcg
     1261 ccgaggtccg actgaagacc acgctgcgtg tgatgcgcgc ggcgatcgac aagtacaagg
     1321 agtacgccga ccaagggctg atcatcctgg aggacgtcga ccagatgggc tacccgcgag
     1381 acctggacga gttggtcgag ggtgtcgagg tcggcgccgg cgactcgccc gacgcgaaga
     1441 agatcaagtt cctccagcgt ttcccggtcg atccgatgac cggcgaagcc gaatggggca
     1501 agcgctcgta tcaggacgac tgggacagcg acagctgggg gggtgagaac gtctacgacg
     1561 tctacagtct gtccgatcgg cgggcgctcg acgggacgta ctatcgcgac tggtgacgtc
     1621 gccgttggtg ccgaaggggg gattcgaacc cccacaccct tgcgggcgcc accccctcaa
     1681 gatggtgtgt ctaccaattc caccacttcg gcaaagtgac gcgcggatta taaccgcgcc
     1741 gcccgcggcc cgtcgacccg cggagcgtca gtcgggcgtc gccggtcctt ccaccggcgg
     1801 cggctcgtcg tcgctcgtcg gcggagcgcc gccggggtta tcggcgggcg ggctgcccgc
     1861 gtcttcaccg gccggctgat cgaccgggga cccgggcgtc gagggcggcg ggacctgctc
     1921 gctccgcgag gtgggtgtgt cctccggcaa gacgctcccg cccttctggc tcacggcgtt
     1981 cgatgcgatc agcgacagcg cgagcgaggt gagcatgaag ctcaccgcgg ccacggtggt
     2041 cagcttggac agcaaggtcg ccgcgccgcg cgtgccgaag gcggtttggc ttccgccacc
     2101 gccgaacgcg ccggccagat cggccttctt gcccgtctgc agcagcacga ccatgatgag
     2161 aaagacggcc acgatcacgt ggacgatcgt cagaagcact ccaagcatgg cgccgacctc
     2221 gggagagcag gattcgtgcc cgaaaacgcc gcgactatac caccccctcc gggaggcggc
     2281 aaacgacgtc gagggctaga gcgtggcgcc ctccgagcgg gggcgcgatc gccgctcggc
     2341 ggcccggctc tcgacgatct cgtcgagcaa tcgtcgttgc gccgggctca acgtctgcag
     2401 cgcccgggag ttgcggcgga tccgctcgcg cttgaggatc tggcggaccg cccgcgcgat
     2461 ccgcggcgcg acgaaggtcg tctcccgctc gtaggcgcgc aacagcagcg gtagctcgtc
     2521 gggttttccg atcagctcga ccgcggcgac catctcgagc tcgaactgct cggaggccag
     2581 cgcatcgcgc agatcgacga tcccgacccg gctccccacg cgcgccagct cgaggtgggc
     2641 tttggcgcgc acccggcgat agcggtccgg atcgcccgag atccgctcga tcacgtcctt
     2701 cagccggtag accatcgact tgtcggcgaa ggcgccgacg acctcgacga ccacctgcag
     2761 cggcagcggc gcctcctcgc ggtcgagcgc gtcgacgatc gcccgcgcgc ggcgtggccc
     2821 caacgccttg agtaccatgc cgacccgtgt gcaatactcg gcgtcggtct gtcgccgccc
     2881 catttccgcg agcaacggcg tgacgacctc cggtcccgag tcgccgagtc gctgcaccgc
     2941 ggcgttccat acgacgtagt cgctcgccgc caagtcggcg atctgggcca gcagccgtcc
     3001 ggacccggtt tccgccgcgc tttcgtcgtc ggccgcgcgc agctccgccg tgcgccggcg
     3061 gatcgtcggt tccgggtcgt tctccagtcg agcgagcaac gcggctcgtt gtccgcgggg
     3121 gatcgcctcg acgagcgcga tcgccgacag ccggacgtgc ttggcctcgc agacgtcgac
     3181 caacaggccg agcgggcgcc ggatcgcgcc ctcgtggccc gcgcggtagt gctcgatcaa
     3241 cgactggacc accgccttgc gcacgccgac gctcgagtcg cggtcgagca cgcgatcgag
     3301 cgcgcgcccg gtcacttccc cgggaaaacg ccccaggctg cgcgaggcga tctgccggat
     3361 ccgtgcgttt cgatcgagca gcatcgccat cagcggctcg cgcgcccggc tgtcccgggt
     3421 caacgccaac aacggcatcg cccgggaacg aacaaggttg tcgtcgccgt ccagggcggc
     3481 gatcagatcc tcgaccgcgc gcgagccgag caccgacagc cgcgcgcgcg ccgcgttgac
     3541 ccgctggcgg tcccggctgc cgagttgacg gaccagttcc cggatctcgt ttgccctcga
     3601 cgaaaccatc atctataaat ctctcacaga ggtgaaccgt gccggacgac tctcggatta
     3661 tcgagtgcac gccgagcgaa gtcaatcgca acgtgatcgg ttcgcgactt cgtgcgcgaa
     3721 gaaaacgatc gacggtcgtg atcgtgtcgt gcatcgcgct gtctctgtcg ctcggcggtt
     3781 gcaacaaggc cgaacgactc tatgccgaag cgcacgagca tatcgagcgg tccgagttcg
     3841 cacaggcagt cgcgttgtac gacgagatca tctcgaccta tccggacagc gagaccgcgg
     3901 cgcgagcgtc gcgcgaggtc gagttgtacc ggggtctggc gacggcggtc gatttctatc
     3961 cggaacgccg catctacgat cagatgatcg agaccgcacg cgcgatctac aagtacgaga
     4021 accgccgcgg gcgctggccc gagtcgttgg accgtctgac ccccgactat ctcgaacggc
     4081 cgccgatcga tccgtggggt cgcgagttgc tctacggcgt caagccccaa cgtcgcggct
     4141 acgtcctcgg ttgctacggc gccgacgggc ggttgggcgg cgacggggcg gcgcgcgact
     4201 ggttcatcga agacggggat ttcgtgcgca agccgtccaa ggccctgtcg tgaggcgatc
     4261 ggtcgtgctg gcggccctcg cgctgctcgg tggcccgctg tccgtcgccg gcgagagcgg
     4321 ctccgactac tcgtgggcgg tggccgtgct gccgtcgggc agggagttca agctcgagat
     4381 cgccgacgac cccgccagcc gctggcgcgg ctaccgctac cgcgagcgac tgccggccga
     4441 cgaggggatg ctgttcatct tcccggtcga acagctgcag tctttcgaga tgcgggactg
     4501 cctcgtcgcg ctcgacatca tcttcctcga cggtgacatg cgtgtggtcg agatcgcgca
     4561 cgaccagcag ccctgccccg ccggcgggtc gtgcccgccg atccagccga tgcaacaggc
     4621 ccgcttcgtc ctcgagatcg ccggcggcag cgcgcggcgc gaggggctcg agcccggcga
     4681 ccgggtgtcg gtgctggccg aaccgccgat tccgtgacct cgctcgtcct ctggctgctg
     4741 ctgttccaga gcgaatcgcc gcccggcgag ccacccgccg atcgcgccgg agcgccgatc
     4801 gccgcggagt cggtcgagat caagggcgcg gatctctccc tgttgacctc ggtgcgcgag
     4861 atcgcccaac gcgtcgagca acagcgcgag cagaagttcc gcagtccgcc gatcgccgtc
     4921 cgaacacccg accacatgcg tgaggtcgcg gcgacgatcc gtgcgctcaa cgtgctggag
     4981 cgcgagcggc tcgaggcccg cggtcgagcg tgggaggatc tcggcctcgg tcagccgtcg
     5041 tcgcccgaga ggctgtacct gctgctggcc ggcgacctgg cggggatcgg cttcgatccc
     5101 caaggcaatc ggttgctcgt cgcgccggag gtgctgaccg acaaggactt ttcgccacag
     5161 gggcccgacg acgagtcctc ggcgacgctg ctgatgatga ccggtgtgcg ggtggacgag
     5221 ccgctggcga gccacctgct gatgcacgtg cgccagcgcg agcggcgcgg cgccgactcg
     5281 ctggccgaga cgaccgacga gctgctggcc agttcggcct gggccgaggg agaggccaac
     5341 ctagtggcga tccgctacct gttcgccggc atgggattgg ccgacgccgt gctcgacccg
     5401 gcgctgagcc cggccgatct gctcgatggc gcgctggtac cgccggcctt ggccaacggc
     5461 gggctcggcg acagcgagcg ggcgctggtt cgattcgtct acgacgaggg cttcttcgcg
     5521 gccaagaccg cattcggcgc gggcggctgg gaggcgctgg atcgggcgat ggcggcgcgc
     5581 accacgacgc gcgacctgat ccaccccggt cgcgaggcgg ccgcgccggt ccgagtcgtc
     5641 gtcgagcggc cgccgcaggc cgacgggctc gagcgagtcg acaccgacac cctcggccag
     5701 caggcgatcg tgacgctggt ctcgaccgtg accggcaagg acaacctggg gttgcaggcc
     5761 ggcgacggtt gggtcggcga cagcgtcgtg cgctgggagg atccgcaaca cgagccccgc
     5821 gacggcgtga ccgtctggtg cacgcgcttg cgcgatgcgc aagcggcggc cgatttcgac
     5881 tacgcggtca gccgcacgct ccaggcacgt ttccccggtc ggccgccgat cgagatcgac
     5941 ggcgatcgag tgatccagac cgccgatcgg gtcgtgcgcc tgcgtcgcgt cggtgtcgac
     6001 gtgacgctgc aggtctccgc gcggagattc gacgagctgc ccgccggcgg cagccgcgcc
     6061 gagcgctgac ggctcaccgg gacttttgcc gggagcggtg ctataccggg gggctcgatc
     6121 ccgacatcgt tgacggcgtc cgttcggtct gggctagaat ccgtgcgcag acgcagagga
     6181 atcgtctgaa tgaacccctt cggtgcccag ctgctcaacg tgatcaccta tctgccggcc
     6241 gtcggcgcgg tgttgatcct cctgctgttc cgcaaggacc agaaccggct cgtcgtccgc
     6301 ttcgccacgg tcgttgccgg ctcgggattc ctcgtcagcc tgccgctgtg gttcggctgg
     6361 cgcgacgccg cgccggatgc gtggggcttc cgcttcgtcc aacaggccga ctggatcgat
     6421 tcgctgggcg tgcgctacat cgtcggcgtc gacgggatct cgatgctgct cgtgctgctg
     6481 acgacgctgc tggggttcgt cgcgatcctg tcggcgtgga acgcggttcg ggtccgcgtc
     6541 agggagttct acgccgcgat gctgctgctg cagaccggca tgatcggcgt gttcgtgtcg
     6601 ctcgatttct tcctgttcta cgttttctgg gaagtgatgc tggttccgat gtacttcatc
     6661 atcggggtct ggggcgggtc gcgcaagatc tacgcggcgg tcaagttctt catctacacg
     6721 ctgctcggat cgctgttgat gctgatcggc atcgtcgccg tctacctcta ccacggccag
     6781 caaaccggca tgtacacctt cgacatccgc tacctgcagg cgatgggcat gtgggccgac
     6841 tggttcgagc tgcagtactg ggtctggctg gcgttcttcg tcggcttcgc gatcaaggtg
     6901 ccgatgttcc cgttccacac ctggctgcct gacgcccacg tcgaggcacc gaccgccggc
     6961 tcggtgatcc tggccggcgt tctgctcaag atgggaacct acggcttcct ccgtttcagt
     7021 ctgccgatga tgcccgaggc gacgcgccac ttcctgccgt gggcgctcgg cttgtcgatc
     7081 gtcggtgtga tctacggtgc gatggtcgca ctgatgcaga aggactggaa gaagctggtg
     7141 gcctactcgt cggtcagcca cttgggcctg acgatggtcg gcgtcttcgc actgaatcac
     7201 cagggtctga ccggcggggt gctgcagatg atcaaccacg gtctgtcgac cggcgccctg
     7261 ttcctgatcg tcggcctggt ctacgaacgg cggcacacgc gcgagatcgc cgagtacggc
     7321 ggactgtgga gggtcatgcc ggttttcgcc gtgctgttca tgatcgcgat gctgtcgtcg
     7381 atcggcatgc cgttgattcc gggcaacggt ttcgtcggcg agttcaccgt gttgatcggg
     7441 gtgttccagc tgccgcagaa gatctgggcc gtgcttgccg ccagcgggat cgtgctcggc
     7501 gcggtgtaca tgctgtggct ctaccagcgc acgatcttcg gtccgctcga ccgggacgag
     7561 aatcgcaagc tgctcgatct cgacttgcgc gagatcgcca cgctggttcc gctgacgctg
     7621 atcgcgttct ggatcggcgt ctaccccaag ccgttcttca acgtgctcga cgagccggtc
     7681 aggcgcctgg tgcagcaggt cgagggccag taccgctatc cacccgaggt cgtgtcgctc
     7741 aggccggagt cgttcgaaga acgtgacgct cgattggcgt cgatccccgg gcccgcggga
     7801 aaacgactga cttcgaccgc cgaccgtcgc ccgatctggc cgcgccggca ccggcggcgt
     7861 caccgtctcc gctcggcgcg tcagacgtag tacaggatca gcagcagtcc gagcagcgcc
     7921 agcacccagg tcaccgagct gccggtggcc caggtgcgcg cgaagacccc cgccgggccg
     7981 tagtgccggt agatgccgga ccgcgcgcgc gcgagctgcc gcgccacggc gcgctcgacc
     8041 gccgcccgca cggtcgccac cgcccgggat gcgcgcagca cgagcgcggg tgccaaccgg
     8101 cggtagagcc aatcggtgtc gaggttgacc gacggtgtct ccgccgggta gcgcccgctc
     8161 ttgatcagca cgccgaaggc gagcgcggcg aacaacaaca gctggtactg cgtgacgacg
     8221 tgagcgccgg tgtatggctg gtagtcgacc gggtagggca gcacggcgta gagcgggaag
     8281 cgatagacgc cgatgaacag gcacgacaac gccgtcaacg ccatcgcgat cagcatgttc
     8341 ggcggagcct ctttacaccg gatccccgaa tcgtgcgcga agaaggcgaa gtacgggatc
     8401 ttgatgcccg agtgttccat cactccggcc gaggcgacga gcaggccgat cgagacccag
     8461 atcagctgct gctcagccac cgcggccagt accatcgact tcgacacgaa gccgctgaac
     8521 agcgggaaag cggagatcga tgcggccccg accaggcaga aagcggtggt gtacggcatc
     8581 gacttgtaga gaccgccgag ctcggtggcc ttgatcgtgc ccgtccggtg gagcaccgcg
     8641 cccatcgcca tgaacaacaa cgccttgtac agcaggtggg cgaacgcgtg gcacaccgcg
     8701 ccgttcagcg cgatctcgct gccgacgccg atgccgacca ccatgaagcc gagctggttg
     8761 ttcaggctgt aggccaacac gcgccgcagg tcgttctcga tcaccgcgaa gaagatcggg
     8821 aacagtgtca tcgccaagcc gatccagatc aggatctcgg tgcccgggaa gccgcgggcc
     8881 agcgcgtaga tcgccagctt ggtggtgaac gcgctgagga agaccgtccc ggtcggcgtc
     8941 gcctcggggt aggcgtcctg cagccagttg tgcagcagcg gaaaggcgca cttgacgccg
     9001 aacgcgagga agatcagcag gccgccggcc ccgtccaggt cgacgcgatc gaactcgatc
     9061 gagccggtgg cgcgggcccg cagcgcgaca ccgccgagca acagcacacc cgagccgacc
     9121 tggacgatca ggtagcgcat tccggcgcgg taggatcact cgttgcggct ggcccagacg
     9181 agaaagaccg aggcgatcgc cgtcagctcc cagtagacga acagcgtgat caggtcgccg
     9241 gcgaacaccg ccgcgatccc ggcgccggcg tagaccaggc cggccacctg ctgcaccgcg
     9301 tccttgacgt gcagggcgta gatcacgctc agcaacagca cgatgctgaa gatcagaccg
     9361 aacagcagac tcagccggtc gacgcgcacg agcgtcagcg tgtagtcgaa gaacgagagc
     9421 tggagatagc tgcccggctc gagccggaac atcgacacta gctgcagcag cccgagcgtc
     9481 ggcagcgcca gcaggtaggc cgcgcgcagc ctgccgcgga gcagcggtac gagccacgcc
     9541 ccgagcaaca ggatcgccgc cggtgggagg ctagcgatca tagtagtctt ccttgcgtcc
     9601 gacgagacgg cgcagctgct ttcccgccag gacgatcaac acgaacacga cgaagccgaa
     9661 gactccgtga aagccgatcc aacgctcgta gacgtagtgc gggtgctcgg ccggaaacca
     9721 cgggtcgatc gcgacggtgg cgagacagac cgcgagcagc gcgtagaaca accgtgtcac
     9781 attgcgcggc gaatcgagcc agtcgggcgt gcggtccccc ggtcgcttca tcattcgtcg
     9841 cccatcacgg ccgcgcgacg ggcgccaaca gccggtagat ctcgtccgcg tagaagaaga
     9901 ttgccaggca accgatcgcg gtcaggcaaa gcggcacgac gcagaacagc ggggcttcgc
     9961 ggatcttgac cgggccatcg agacccggcg gggcgcagaa gaagccgcgg atcaccaccg
    10021 gcatcagata ggcgatgttc aacagcgagc tgaccatcag caccgcgatc agcgacagcc
    10081 gctgggcgtc ggcgctgccg agcatcagca gccacttgct ccagctgccc cccatcggcg
    10141 gcaggccgat gatgctcagc gacccgagca ggaaggcgaa ggtcgtcacc ggcatcacgc
    10201 ggccgatacc gcgcatctcg ctgatctccg tcttgtcggc cgcgatgtag atcgcgccgg
    10261 cgcagaagaa cagcgtgatc ttgcccagcg catgcgtcgc gatgtgcatc ccgccgccga
    10321 tcacgcccca gcggttggcc agcgccgcgc cgagcacgat gtacgacagc tggctgatcg
    10381 tcgagtaggc caatctggcc ttgaggttgt ccttggtcag cgcgatcagc gaggccagca
    10441 cgatcgtcag cgctgcgacg tgcatcagcc agacactggc ggctgcgttc gccaggaagt
    10501 cgatgccgaa gacgtagacg acgaccttga ggatgctgaa cacaccggcc ttgaccacgg
    10561 cgacggcgtg gagcagcgcg ctgaccggcg tcggggccac catcgcggcg ggcagccagc
    10621 ggtggaacgg catcagcgcg gccttgccga tgccgaaggc gtagagaccg aggaggatcg
    10681 cggccgtacg cgggtcgacc cgaccgtcga ggatgccgcc cggccggaac tcgagcgtgc
    10741 cggtgtagca ccaggtccag atcaccgcca acagcaacag gccgatcgac gtgctgagca
    10801 gcacgccgag atagatccgt ccggcacgca gtgcgccttc ggtgcgatgg tggatcacca
    10861 gcggataggt cgacagcgtc agcagctcat aaaacacgaa cagcgtcagc agattccgcg
    10921 cgaaggcgat tcccatcgtc gccgaaatcg acaaggcgaa gcaggcgtaa aaccggctct
    10981 ggtgatcttc gtcgtgtccg cgcatgtagc cgatcgagta ggcggtggtg acgacccaca
    11041 gcgtcgatgc gaccaacgcg aacagcaggc ccagcggctc gacatcgaac gccagcgcga
    11101 tgccgggcat cagctggacc agctcgagcg acgccgagcc ggtcagcgcg atgcggacga
    11161 cgaccagcac cagcgcgccg ccgatgacca gcgtcgtcgc ctcgcgcaga tcgcggtgcc
    11221 ggtgcgccac cgccgcggtc agcgcgccgg ccgccggcag caggatcgcc agcgcgatca
    11281 acccgccggg gctcacgggg cgcccccgag caacgagatc gccgcacgcc gcgcgacgcc
    11341 gacggaaaac gcgctccaca cgccgaacag cacgacggcg ccgatcagca cccaggtcgg
    11401 gccgagcatc gacgccgggg cttcgccgcc ggctgccggc tcgcgcggct cgccgaagta
    11461 cagcgtctcg acgacgcgcc aaacgtagac cagcgccagc aacgagctga gcagcacgag
    11521 cgcggcgacc ggccacagcc cgctctcgag cgccgccgcg atcaggtacc acttgctgac
    11581 gaacccggcg gtcagcggca cgccgatcag ccccaggccg ccgacgagcc aggcgaacgc
    11641 ggtcaacggc atccgccggg ccaacccgcg catctcgtcg atcgagacgc cgccggcgcg
    11701 tagcgcgatg catcccatcg ccatgaacag cgcgcccttc atcagcgcat gattgaacat
    11761 gtgcacgatc gtcgcggtca agccgctgag cgtgcccaga ccgatgccga gcaccatgta
    11821 gccgacctgt gcgatgctgg agtacgccag cagccgtttg acgtcacgct ggaagatcgc
    11881 cacggtcgag gcaaaaaagg cgccggcgag cgccagcggc agcaggatcc acccgagccg
    11941 cagacgctcg aagacgaatc ccgcgccgaa cacggtgaag gcgaatcgca gcatgatgta
    12001 gaccgatacc ttggtcgccg tcgccgccag aaacgacgac acgaccgacg gggcgtaggt
    12061 gtaggcgttc ggcagccaga gatgcagcgg atagagcgcc atcttcaggc cgatgccgac
    12121 gacgagaaag ccgaacgcgg cgaccagcgt ccgcgtgccg ctgaccgacg gcagtcgcac
    12181 cgccatgtcg gccatgttca gcgtgccggt catg
//
LOCUS       DASPGN010000577         2240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_344365, whole genome shotgun sequence.
ACCESSION   DASPGN010000577 DASPGN010000000
VERSION     DASPGN010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344365"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1965
                     /gene="fusA"
                     /locus_tag="VD788_16630"
     CDS             <1..1965
                     /gene="fusA"
                     /locus_tag="VD788_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXV77937.1"
                     /translation="HDGAAVMDYMEQEQERGITITAAATTCFWKGMDSHHPEHRINII
                     DTPGHVDFTIEVERSLRVLDSAVAVYCAVSGVQPQSETVWRQMNKYRVPRIAFVNKMD
                     RAGANFLRCVEQIRTRLKGNPVVIQLPIGAEEGFAGIVDLVRMKSVWWDEESQGMRFE
                     YRDIPADMKAQCDEYRMKLVEAAAEGNDELLNKYLEGAELTVDEIKKGLRQRAIRNEI
                     CIVTCGSAFKNKGVQAVLDAVIDYMPSPIEVPDVKGQTEEGAEIARKSADDAPFSALA
                     FKILNDPFVGNLTFFRVYSGTLSSGDQVYVPNRSRKERIGRLLQMHASEREEIKEVRA
                     GDIAAAVGLKDVTTGDTLCDPDDVIVLEKMEFPDPVISVAVEPKTKADQEKMGIALQR
                     LAKEDPSFRVATDQESGQTIISGMGELHLEIIVDRMKREFKVEANVGKPQVAYRETIR
                     KKVEQEGKFVRQSGGRGQYGHVWITIEPLEEGKGYEFVNEVVGGAIPREYIPAVDKGI
                     KEALGNGVIAGFPIVDVKVALTDGSYHDVDSSEMAFKIAGSMAFKEGFAKAKPVLLEP
                     IMNVEVVTPEEYSGDVIGDLNRRRGQITGMDESPSGKIVTADVPLAEMFGYATSVRSM
                     SQGRATFTMEFKKYVEVPNNVAETVLKKAS"
     gene            1980..>2240
                     /locus_tag="VD788_16635"
     CDS             1980..>2240
                     /locus_tag="VD788_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXV77938.1"
                     /translation="MAKEKFVRTKPHVNVGTIGHVDHGKTTLTAALTKVMAEKNGGEF
                     TAYDQIDKAPEEKARGITIATAHVEYQSAARHYAHVDCPGHAD"
BASE COUNT          454 a          709 c          764 g          313 t
ORIGIN      
        1 cacgacggcg ccgccgtgat ggactacatg gagcaggaac aggagcgcgg catcacgatc
       61 accgccgccg ccacgacctg cttctggaag gggatggact cgcaccaccc cgagcaccgc
      121 atcaacatca tcgacacgcc cgggcacgtc gacttcacga tcgaggtcga gcgctcgctg
      181 cgcgtgctcg actcggcggt cgcggtgtac tgcgccgtgt ccggcgtgca gccccagtcc
      241 gagacggtct ggcggcagat gaacaagtac cgcgtgccgc gcatcgcctt cgtcaacaag
      301 atggaccgcg ccggcgccaa cttcctgcgc tgcgtggagc agatccgcac gcgcctgaag
      361 ggcaacccgg tcgtgatcca gctgccgatc ggcgccgagg aaggcttcgc cggcatcgtc
      421 gacctggtcc gaatgaagtc cgtctggtgg gacgaggaat cccagggcat gcgcttcgag
      481 taccgcgaca tccccgcgga catgaaagcg cagtgcgacg agtaccgcat gaagctggtc
      541 gaggccgccg ccgagggcaa cgacgagctg ctgaacaagt acctcgaggg cgccgagctc
      601 acggtcgacg agatcaagaa gggcctgcgc cagcgcgcca tccgcaacga gatctgcatc
      661 gtcacctgcg gctccgcctt caagaacaag ggcgtgcagg ccgtgctgga cgcggtgatc
      721 gactacatgc cttcgccgat cgaggtcccc gacgtcaagg gccagaccga ggaaggcgcc
      781 gagatcgcgc gcaagtccgc ggacgacgcg ccgttctctg cgctcgcgtt caagatcctg
      841 aacgacccct tcgtcggcaa cctgaccttc ttccgcgtct actcgggcac gctgtcctcc
      901 ggcgaccagg tgtacgtgcc gaaccgctcg cgcaaggaac gcatcggccg cctgctgcag
      961 atgcacgcga gcgagcgcga ggagatcaag gaagtccgcg ccggcgacat cgccgcggcc
     1021 gtgggcctca aggacgtgac gactggcgac accctgtgcg acccggacga cgtcatcgtg
     1081 ctcgagaaga tggagttccc ggacccggtg atctcggtcg ccgtggagcc gaagaccaag
     1141 gccgaccagg agaagatggg gatcgcgctg cagcgcctcg ccaaggaaga tccgtcgttc
     1201 cgcgtcgcca ccgaccagga gtcgggccag acgatcatct ccggcatggg cgagctgcac
     1261 ctggagatca tcgtcgaccg catgaagcgc gagttcaagg tcgaggcgaa cgtcggcaag
     1321 ccgcaggtcg cctaccgcga gacgatccgc aagaaggtcg agcaggaagg caagttcgtg
     1381 cgccagtccg gcggacgcgg ccagtacggc cacgtctgga tcacgatcga gccgctcgag
     1441 gaaggcaagg gctacgagtt cgtcaacgag gtcgtgggcg gcgcgatccc gcgcgagtac
     1501 atcccggccg tcgacaaggg catcaaggaa gcgctgggca acggcgtcat cgccggcttc
     1561 ccgatcgtcg acgtgaaggt cgcgctgacc gacggctcgt accacgacgt cgactcgagt
     1621 gaaatggcgt tcaagatcgc gggctcgatg gccttcaagg agggcttcgc caaggcgaag
     1681 ccggtgctgc tcgagccgat catgaacgtc gaggtcgtga cgcccgagga gtactcgggc
     1741 gacgtgatcg gcgacctgaa ccgccgccgc ggccagatca ccggcatgga cgagtcgccg
     1801 tccgggaaga tcgtgaccgc cgacgtcccg ctcgcggaga tgttcggcta cgcgacgtcc
     1861 gtgcgctcga tgagccaggg ccgcgccacg ttcacgatgg agttcaagaa atacgtcgag
     1921 gtgccgaaca acgtcgccga gacggtcctc aagaaagcct cttaagggaa gatcgcgcaa
     1981 tggccaagga aaagttcgta cgtacgaagc cccatgtgaa cgtggggacg attgggcacg
     2041 tggatcacgg caagacgacg ctgacggcgg cgttgaccaa ggtgatggcg gagaagaacg
     2101 gcggggagtt cacggcgtac gaccagattg acaaggcgcc ggaggagaag gcgcgcggga
     2161 tcacgattgc gacggcgcac gtggagtacc agagtgcggc gcggcactac gcgcacgtgg
     2221 attgtccggg gcatgccgac
//
LOCUS       DASPGN010000578         4183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_812016, whole genome shotgun sequence.
ACCESSION   DASPGN010000578 DASPGN010000000
VERSION     DASPGN010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812016"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VD788_16640"
     CDS             complement(<1..495)
                     /locus_tag="VD788_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77939.1"
                     /translation="MGAFFLCVRERERESADVLGRGLASLRAQGFAQPTRITTTGFDI
                     VAFPKYRGGSVQLAKRDDDNFALLTGTSIFEHGVGEQAAKHFLEDFQFEAPAWDEIHG
                     HYCAIVCRRGRLRLFVDRLGTYKVYADRARSVVSSSFLAVCDAIGRLEIDSQAVYEYA
                     FLGGT"
     gene            complement(515..1741)
                     /locus_tag="VD788_16645"
     CDS             complement(515..1741)
                     /locus_tag="VD788_16645"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXV77940.1"
                     /translation="MPIDADRPIRLLTFSGLYPSTARPTHGIFVEQRLRQLLDSGRVE
                     ARVVAPVPWFPFRHRIFGEYSRIAATPRRATRHGVEVEYARFPVLPKIGAWLTPYLLA
                     AAAAPAIRRIVDRGFDFDVLDAHYFFPDGVAAARIGRRFGKPTVITARGSDVNVLTRF
                     RAPRRMILRAAEQAAAVVTVSDALRQSLEDLGVDPGRLHVLRNGVDLGRFRPVEPRPA
                     GWPWPDGRRTILSVGNLIEGKGHHLVIRALERLDGLRLVVVGAGRMRGELSELVSRLG
                     LSARVHLAGTVDQTELPAYYSTADALVLASRREGMPNVVLEALACGCPVVATAVGGIP
                     EIIAGSSAGMLLADDDPETIAAAVGDLLARPIDRRAIRQFAERFGWEKTVERQIDLFS
                     RIATAQTTVTAIADER"
     gene            complement(1762..2817)
                     /locus_tag="VD788_16650"
     CDS             complement(1762..2817)
                     /locus_tag="VD788_16650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXV77941.1"
                     /translation="MWSRVKLAVATLLYHSRLLDLLNRLINRYRVDRVRRLGPVPAIR
                     RRRVGDVQILIYHRVGSQADGFLPPLPIETFERQMRYVAANCRVLSLDDAADAIRRRD
                     VPPNAVVITFDDGYRDNYTTALPVLSTYSLPATIFVVTGAIGNGGVIWHDRVFRAFST
                     TTCRELREFGPRAECYPLRTPDQLAYAERSVLRYLKSLPPERRDREIARLIDRLDTGD
                     VDRADGLMLGWEELREMNARGVSVGAHTVSHPILSRVSPEQARREIAESKRTIEGELG
                     CPVRNFAYPNGARVDFDDTTKTILSECGFDCALTTIFGTNDDDSTDRPDMLELKRMDL
                     VDGRLPLFATKLNVYRW"
     gene            complement(2846..3880)
                     /locus_tag="VD788_16655"
     CDS             complement(2846..3880)
                     /locus_tag="VD788_16655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV77942.1"
                     /translation="MKRTLFEADSQHAAVELRPPAERVALSVVIPVSERVDRLVETHR
                     RLVEVLAKLDKSHEIIYVLDGPHPAVIEQLNEAKRRDTTVHLITLNRWFGEAAALAVG
                     FERSVGETIITLPSYPQVEPAELPRMLAAYEQGDVDLLIARRDPRVDSRFNRLQSRVF
                     HGLTRWLTGTHYRDVSCGVKLMKRSVAAEVEIYGDLHRFFPLLADQRGFKVEEIAVPQ
                     SPEDAGRRIYRPGLYLRRLLDLLTLFFIFKFTKKPLRFFGLLGSGLFGAGGAILIYLA
                     AYRLLQFGPIANRPLLILGALLIVLGLQLFSIGLLGEIVIFTHAGGAKDYQIREVRQG
                     GGRFPGSDRS"
     gene            complement(3861..>4183)
                     /locus_tag="VD788_16660"
     CDS             complement(3861..>4183)
                     /locus_tag="VD788_16660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77943.1"
                     /translation="AMDIVTVKMITSFATRPSAWFALLSTPALLAGMLALVLAQGARI
                     SGVTEEWLVLATVGLLLLFLGVNLVSLGILGELCVSTGDVSPRRILAPLARSVEAGNE
                     TNAV"
BASE COUNT          727 a         1441 c         1403 g          612 t
ORIGIN      
        1 cgtcccgccc aggaaggcgt actcgtacac cgcctgtgag tcgatctcca gccgaccgat
       61 cgcgtcgcag acggcgagga acgagctcga gacgacggat cgagcgcgat cggcgtacac
      121 cttgtacgtg ccgaggcggt cgacgaacaa gcgcagccgg ccgcggcggc agacgatcgc
      181 gcagtagtgc ccgtggatct cgtcccacgc cggggcttcg aactggaaat cctcgaggaa
      241 atgcttcgcc gcctgctcgc cgacgccgtg ctcgaagatc gaggtcccgg tgagcagcgc
      301 gaagttgtca tcgtcgcgtt tcgccagttg cacgctcccg ccccggtact tcggaaaggc
      361 gacgatgtcg aacccggtgg tcgtgatccg ggtgggttgc gcgaacccct gcgcccgcag
      421 cgaggcgagc ccacgtccga ggacgtcggc ggattcgcgc tcgcgctctc gcacgcacag
      481 aaagaaagcg cccacggaaa cctcgtcttg ccggctaccg ttcgtcggcg atcgccgtga
      541 ccgtcgtttg tgccgtcgcg atcctcgaga acaggtcgat ctggcgttcc accgtcttct
      601 cccagccgaa ccgctcggcg aactgccgaa tcgcgcgccg gtcgatcggc cgggccagca
      661 gatcgccgac cgccgctgcg atcgtctccg ggtcgtcgtc ggccagcagc ataccagcgg
      721 aggatccggc gatgatctcc ggaatcccgc cgaccgccgt cgccacgacg gggcagccgc
      781 acgccagcgc ctcgagtacg acgttgggca tcccctcgcg acgcgaggcg agcaccagcg
      841 catcggcggt gctgtagtac gccggcagct ccgtctgatc gaccgtccct gccaaatgca
      901 cacgcgccga aagaccgagt cggctcacca gctccgacag ctcgccgcgc attctccccg
      961 caccgacgac gaccagccgc aggccgtcga ggcgctcgag cgcgcggatc acgagatgat
     1021 ggcccttgcc ttcgatcaag ttgccgacgg agaggatcgt ccgccgaccg tcgggccagg
     1081 gccaaccggc gggccgcggc tcgaccggcc ggaagcggcc gaggtcgacg ccgttgcgca
     1141 gcacgtggag ccgaccgggg tcgacgccga ggtcctcgag cgactggcgc agcgcgtcgc
     1201 tgacggtgac gaccgcggcc gcctgctccg ccgcccgaag gatcatccgg cgcggcgcgc
     1261 gaaagcgggt cagcacgttg acgtcgctgc cccgcgcggt gatcaccgtc ggcttgccga
     1321 atcttctgcc gatccgcgcg gctgccaccc cgtcggggaa gaagtagtgg gcgtcgagca
     1381 cgtcgaaatc gaacccgcgg tcgacgatcc ggcggatcgc gggcgccgcc gccgccgcga
     1441 gcaggtacgg ggtcagccat gcgccgatct tcggcaggac gggaaagcgc gcgtactcga
     1501 cctcgacgcc gtggcgcgtc gcgcggcgcg gtgtggcggc gatcctggaa tactcgccga
     1561 agatgcggtg acggaacggg aaccacggca ccggggcgac gactcgtgcc tcgacgcggc
     1621 cgctgtcgag cagctgccgc agacgctgct cgacgaagat cccgtgcgtc ggcctggccg
     1681 tgctcgggta gagcccggag aaggtcaaga ggcggatcgg tcgatcggcg tcaatcggca
     1741 cccggactcc gtcggcgccg ctcaccaccg atacacgttg agcttggtgg cgaacagcgg
     1801 cagccgtccg tcgacgaggt ccatccgctt cagctccagc atgtccggcc ggtcggtcga
     1861 atcgtcgtcg ttggttccga agatcgtcgt cagcgcacaa tcgaacccgc attccgacag
     1921 gatcgtcttg gtcgtgtcgt cgaagtccac gcgtgccccg ttggggtagg cgaagtttcg
     1981 caccggacaa cccagctcgc cttcgatcgt ccgtttcgat tcggcgatct cacggcgtgc
     2041 ctgctcgggt gacacgcgcg acaggatcgg gtggctgacc gtgtgcgcgc cgaccgaaac
     2101 gccacgcgcg ttcatctccc gcagctcctc ccaaccgagc atcaggccgt ccgcccgatc
     2161 gacgtcgccg gtgtcgagcc tgtcgatcag ccgggcgatc tcgcgatccc gccgctcggg
     2221 cggcagcgac ttcaagtacc gcaggaccga ccgctcggcg tacgcgagct ggtcgggcgt
     2281 gcggagcgga taacactccg ctcgcggccc gaactcgcga agctcgcggc aggtcgtcgt
     2341 gctgaacgcg cggaacacgc gatcatgcca gatcaccccg ccgttcccga tcgccccggt
     2401 gacgacgaag atcgtcgcgg gcaacgagta tgtcgaaagg accggcagcg cggtcgtgta
     2461 gttgtcgcgg taaccgtcgt cgaacgtgat gacgacggcg ttcggcggaa cgtcccggcg
     2521 gcgtatcgcg tccgcggcgt cgtcgaggct gagcacgcga cagttcgccg ccacgtagcg
     2581 catctggcgt tcgaaggtct cgatgggcaa cggtggcagg aatccgtcgg cctgcgagcc
     2641 gacgcggtga tagatcaaga tctgcacgtc gcccacacgg cggcgtctga tcgccggaac
     2701 gggccccaac cggcgaacgc gatcgacccg gtaccggttg atcagccggt tgagcaggtc
     2761 gagcagccgc gagtggtaga gcagcgtcgc gacggcgagc tttacccggc tccacatgct
     2821 cgaacgggtc ccaaccgcgt ggtcgtcaag atcggtccga gcccggaaac cggccgcccc
     2881 cctggcgaac ctcgcggatc tgatagtcct tcgcgccgcc ggcgtgggtg aagatcacga
     2941 tctcgccgag cagaccgatc gagaacaact gaagacccag cacgatcagc agcgcaccca
     3001 ggatcaagag cgggcggttc gcgatcgggc cgaattggag caggcggtac gcagcgaggt
     3061 aaatcaggat cgccccgccg gcaccgaaca gcccggagcc gagcaggccg aagaaccgca
     3121 gcggcttctt ggtgaacttg aagatgaaga acagcgtgag caggtcgagc agccgcctga
     3181 ggtacaaccc cggccggtag attctccgcc ccgcgtcctc cgggctctgc gggaccgcga
     3241 tctcctcgac cttgaacccg cgctgatcgg cgagcagcgg gaagaaccga tgaagatcgc
     3301 cgtagatctc gacctcggcg gcgacgctcc gcttcatcag cttgacgccg cagctgacgt
     3361 cgcggtagtg ggtcccggtc agccagcggg tcaacccgtg aaagacgcgg gactgcagcc
     3421 gattgaaccg cgagtcgacc cgcggatcgc gacgcgcgat cagcaggtcg acgtcaccct
     3481 gctcgtaggc cgcgagcatc cgtggcagct cggccggctc gacctgcgga taggacggca
     3541 gggtgatgat cgtctcgccg acggaccgct cgaacccgac ggccaacgcc gcggcctcgc
     3601 cgaaccaacg gttcagcgtg atcaggtgga cggtcgtatc ccgtcgcttg gcctcgttca
     3661 actgctcgat cacggcgggg tgcggtccgt cgagcacgta gatgatctcg tgggacttgt
     3721 cgagcttggc cagcacttcg accaaccggc gatgggtctc gaccaggcgg tcgacacgct
     3781 ccgagaccgg gatcacgacc gacagcgcga cccgttcggc gggcggccgc agctcgaccg
     3841 ccgcgtgttg cgaatcggct tcaaacagcg ttcgtttcat tccctgcctc taccgaccgc
     3901 gcgagcggag ccaagattcg tcgcggggaa acgtctcccg tgctgacgca cagctccccc
     3961 agaatcccga gcgacaccag gttgacgccg aggaacagca gcagcagtcc gacggtcgcg
     4021 agcaccagcc actcctcggt cacgccggag atccgggcgc cctgcgccag cacgagcgcg
     4081 agcatccccg cgagcagcgc cggcgtgctc agcaaggcga accaggccga cggccgggtg
     4141 gcgaagctgg tgatcatctt gactgtcacg atgtccatcg cga
//
LOCUS       DASPGN010000579         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_929045, whole genome shotgun sequence.
ACCESSION   DASPGN010000579 DASPGN010000000
VERSION     DASPGN010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929045"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2301
                     /locus_tag="VD788_16665"
     CDS             <1..>2301
                     /locus_tag="VD788_16665"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HXV77944.1"
                     /translation="FVRSDLEFILEQIRIAEAHAAGTPLEQLIANPTLPFGLRTVDGS
                     YNNLVQGQSEFGAADTLFPRMLEPDFRDAESGTSYEQTSGLVIDSSPRTISNLIVDQS
                     NNNPAAVAADDADGVDDDFIANVTPDAGLSAPFNSWFTFFGQFFDHGLDLVTKGGGTI
                     FMPLKPDDPLYVEGSPTNFMVLTRATNQPGPDGVLGTADDIHEHTNTTTPFVDQNQTY
                     TSHPSHQVFLREYVLDSDGKPVSTGRLLENRAPGPDGLYYTGDDVLLGGLTTWAVVKA
                     QAAKLLGIELTDADVTNLPLLATDPYGQFLPGENGFPQLVQLDNSLLEGNPAAPVSTA
                     NAVRTGHAFLDDIAHSAAPRSSSGVLLTPDDDLVAGIDDHLSTTYDDELLGAHYITGD
                     GRGNENIGLTSVHHVFHSEHNRLADQVKDVVLATNDLAFLSEWLRPGTAPETFPTTPA
                     ELDALQWNGERLFQAARFGTEMQYQHLVFEEFARRIQPNINIFDAYETDIDAAIVAEF
                     AHTVYRFGHSMLTETVDRFDADGNPVEAPGGGQLGLIEAFLNPLAYADRETAPGVAAG
                     EVVRGMTRQVANEIDEFVTDALRNNLLGLPLDLATINLARGRDTGVPSLNAARREFFE
                     GTGGDTQLRPYESWVDFGLAIKHPESLVNFIAAYGLHPSILDATTLADKRAAALAIIT
                     GGVDAPLDSIEFLNSTGIWASAPDGVTTTGLDNVDFWIGGLAEKQMPFGGVLGSTFNF
                     VFETQLEKLQDGDRFYYLSRTLGLNF"
BASE COUNT          399 a          861 c          687 g          354 t
ORIGIN      
        1 gttcgtccgc tccgatctcg aattcatcct cgagcagatc cgcatcgccg aggcgcacgc
       61 cgccggcaca ccgctggaac agctcatcgc caacccgacc ctgccgttcg gcctgcgtac
      121 ggtcgacggg tcgtacaaca acctggtcca gggccagtcc gaattcggcg cggccgacac
      181 cctgtttccg cggatgctcg agccggactt ccgcgacgcg gagagcggca cctcctacga
      241 acagaccagc gggctcgtca tcgactcgtc gccgcgcacg atcagcaatc tgatcgtcga
      301 tcagtcgaac aacaacccgg cggccgtcgc cgccgacgat gcggacggcg tcgatgacga
      361 cttcatcgcc aacgtcacgc cggacgccgg cctgtcggcg ccgttcaact cctggttcac
      421 tttcttcggc cagttcttcg accacggcct cgatctggtg accaagggcg gcgggacgat
      481 cttcatgccc ctcaagcccg atgatccgct ctacgtcgaa ggcagcccca cgaacttcat
      541 ggtcctgacg cgggcgacca atcagcccgg gcccgacggc gtgctcggca cggccgacga
      601 catccacgag cataccaaca cgacgacgcc gttcgtcgat cagaaccaga cctacacctc
      661 gcacccgtcg caccaggtgt tcctgcgcga gtacgtgctc gacagcgacg gcaagccggt
      721 gtcgaccggc cggctgctcg agaaccgcgc cccgggtccc gacggcctgt actacaccgg
      781 cgacgacgtc ctgctcggcg ggctcaccac ctgggcggtc gtcaaggcgc aggccgcgaa
      841 gctgctcggc atcgagctca ctgacgccga cgtgaccaac ctgccgctgc tggcgaccga
      901 cccgtacggc cagttcctgc ccggtgaaaa cggattcccg cagctggtgc agctcgacaa
      961 tagcctgctg gagggcaatc cggccgcgcc agtgagcacc gccaacgcgg tccgtactgg
     1021 ccacgccttc ctcgatgaca tcgcccactc ggcggccccg cgctcgtcca gcggcgtcct
     1081 gctgacgccc gacgacgacc tggtcgccgg tatcgacgac cacctctcca ccacctacga
     1141 cgacgagctc ctgggcgccc actacatcac cggcgacggc cgcggcaacg agaacatcgg
     1201 cctgacctcg gtccaccacg tgttccactc ggagcacaac cgcctggccg accaggtcaa
     1261 ggacgtggtg ctggccacca acgacctcgc gttcctcagc gagtggctgc gccccgggac
     1321 ggccccggag acgttcccga cgacgcccgc cgagctcgac gcgctgcaat ggaacggcga
     1381 gcgcctgttc caggcagccc ggttcggcac cgagatgcaa taccagcacc tcgtgttcga
     1441 ggagttcgcg cggcggatcc agccgaacat caacatcttc gacgcctacg agaccgacat
     1501 cgacgcggcg atcgtcgccg agttcgccca cacggtctac cgcttcggcc actcgatgct
     1561 gaccgagacg gtcgaccgct tcgacgccga cggcaacccg gtcgaggcgc ccggcggcgg
     1621 ccagttgggc ctgatcgagg ctttcctgaa cccgctggct tacgccgatc gggagacggc
     1681 ccccggcgtc gccgcgggcg aggtcgtgcg cggcatgacc cgccaagtcg ccaacgagat
     1741 cgacgagttt gtcaccgatg ccttgcgcaa caacctgctc gggctgccgc tcgatctcgc
     1801 caccatcaac ctggcgcgcg gtcgcgatac cggcgtcccg tcgctgaacg cggcccggcg
     1861 tgagttcttc gaaggcaccg gcggcgacac ccagctccgc ccgtacgaga gctgggtcga
     1921 cttcgggctc gcaatcaagc atcccgagtc gctggtcaac tttatcgccg cctacgggct
     1981 gcacccgtcg atcctcgacg ccaccacgct ggccgacaaa cgcgcggcag cgttggcgat
     2041 catcaccggc ggcgtcgacg cgccgcttga ctcgatcgag ttcctcaaca gcaccggcat
     2101 ctgggcgagc gcgcccgacg gcgtgacgac caccggtctc gacaacgtcg acttctggat
     2161 cggcggcctc gccgagaagc agatgccctt cggcggcgtg ctcggctcga ccttcaactt
     2221 cgtcttcgag acccaactcg agaagctcca ggacggcgat cggttctact acctgtcgcg
     2281 gacgttgggt ctgaacttct t
//
LOCUS       DASPGN010000580         4709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_109568, whole genome shotgun sequence.
ACCESSION   DASPGN010000580 DASPGN010000000
VERSION     DASPGN010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109568"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VD788_16670"
     CDS             <1..793
                     /locus_tag="VD788_16670"
                     /inference="COORDINATES: protein motif:HMM:NF024136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HXV77945.1"
                     /translation="HGAVNTLATTGLLFALFTSWSINLHRTPLAEVAGLDTRPASDRD
                     LTTLCERLVERANLARAEVHENESDGTMRLNDAPGQALARTWLAFHRAEQLHRPLFRG
                     RVGRVKRLTVLSEPATRLGFGGLYLAVTGEPTVSASLPPAGLPFAACHQLGHQVGWAG
                     EADASFAGWLACTSHPDADFRYAAHLAALDGAMDALLAIDPSAWRPLIERVTPEVRND
                     WRTLDRIGVGCPQLDLPTTGRPSSWIDLLLAHERREAAVREASLR"
     gene            884..1987
                     /locus_tag="VD788_16675"
     CDS             884..1987
                     /locus_tag="VD788_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXV77946.1"
                     /translation="MKIRAAVLEAFGEPLVVQELDLAGPAAGEVLVRLAACGVCHTDL
                     YSASGADPSGYAPCVLGHEGAGVVEAVGAGVNGLRPGDHVITLFSPECGECIHCTSGR
                     TNICLAIREQQGKGLLPDGTTRLSRDGEPMRHFMGCSTFAEATVMPEIALARIDPRAP
                     LDSVCTLACGATTGLAGALWKAEVEPGSTCAVFGVGMVGLGAVVGCRLAGAERIIAVD
                     LAPRRLELARRYGATETIVAGEDSVRRILDRTGGFGADYTFEATGLVGVMRQAVEATR
                     IGWGLCTVLGVAGKGETLDIVPRLLITGRRVQGGSFGGARGRTHVPQLVDMYLAGKID
                     LDGFVSHRLTLDEVNRGFELMRAQDGIRSVVVF"
     gene            2018..2770
                     /locus_tag="VD788_16680"
     CDS             2018..2770
                     /locus_tag="VD788_16680"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV77947.1"
                     /translation="MIIHKTMSDGWLSNTWLVGDEPGGHAVLIDTGGPMDPIVRAIEQ
                     HRLTVTHVLCTHHHIDHVQHNSAYKRRLGCPICGHHSESELFGGLDLALDDGDELVTG
                     GLHLRVLHTPGHTRGMLAFVVNDERLFTGDTLFRRTVGGTRAPGHTSFEDLHHSIMDV
                     LMKFPKGTKIHPGHTDVSTVGEEWEENPFIRMWRGVDRPGERRCTAFGEPATLLLRAR
                     DYDGGSKCWVRFDDGGKLDVVPGSQVRTDDGM"
     gene            2905..4419
                     /locus_tag="VD788_16685"
     CDS             2905..4419
                     /locus_tag="VD788_16685"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HXV77948.1"
                     /translation="MLRLGSPLRARSAVVAPAIGLSLVAASCGEGHAPAPACPDLSWT
                     GPRPGTSLVLVVNDTLRRDRVGIYGGPDQTPTFDAFARGGMLFERATSQAPWTSPAIA
                     SLFSGLYPSAHGVRSHPYLRATADGATTASATRKVDVLGDDLTLLAELLDDAGYHTAA
                     IVGNPWLERRFGFAQGFDFYDDSFADWDAPGRDVAGAALDWLAGVPDGEPYFLYVHTM
                     DTHRPYPSVSREALHARRDELVDDRRPLDPAVRQSIADLVFLEGAGKAVRAGLTPSLS
                     LIELAYDAGVRAFDDSLSVLLEGMAGEAEDETALIVLSDHGEALFSRGWGNHGHGLYE
                     DEVAIPMAARLPGVRAERDRVGCPTGLVDVLPTVCSYLDLDCPDDLGGESFLAPRKQV
                     GSLADRRLLVEGVMHKPQNRAVRDRRYKLLLQPQPDLSGTTDALFDTSIDPGETTNLL
                     EPGSLTPRHAEVRAELERAAREMLSRSAPASNDDETVPLDAATERRLRSLGYIR"
     gene            4473..>4709
                     /locus_tag="VD788_16690"
     CDS             4473..>4709
                     /locus_tag="VD788_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77949.1"
                     /translation="MTGERPTRGESWGVWLLLAVVCGMTLPELAEPPATGLDPSWRVG
                     LHLARERGLTVGDDLFFTYGPWGYLTVPLTMTRPL"
BASE COUNT          670 a         1615 c         1676 g          748 t
ORIGIN      
        1 gcacggagcg gtcaatacgc tcgcgacgac cggtctcctg ttcgcgctgt tcacgtcgtg
       61 gtcgatcaac ctccatcgca cgccgctggc cgaggtcgcc ggtctcgaca cgcggccggc
      121 gtccgaccgc gacttgacca cgctgtgcga gcggctggtc gagcgggcca acctcgcgcg
      181 ggccgaggtt cacgagaacg agagcgacgg aacgatgaga ctgaacgacg ctccggggca
      241 ggcgctggcg cggacctggc tcgccttcca tcgcgccgag cagctccacc ggccgctgtt
      301 ccgcggtcgt gtcgggcggg tcaagcgcct gaccgtcctg tccgagccgg cgacgcggct
      361 cggcttcggc ggtctgtacc tcgccgtgac cggcgagccg acggtcagcg cgtcgctgcc
      421 gccggccggt ctgccgttcg ccgcgtgtca tcaactgggg caccaggtcg gctgggccgg
      481 tgaagcggac gcgagcttcg ccggctggct ggcctgcacc tctcatccgg acgccgactt
      541 ccgctacgcc gcacatctcg cggcgctcga cggggcgatg gatgcgctgt tggcgatcga
      601 tccgtcggcc tggcggccgc tgatcgagcg cgtgacgccc gaggtcagga acgactggcg
      661 cacgctcgac cggatcgggg tcggctgccc gcagctcgac ctgccgacga ccggccggcc
      721 gtcgagttgg atcgatctgc ttctcgcgca cgaacgccgg gaagcggcgg tccgcgaagc
      781 ttccctccgc tgacgccctc ggccccgtgc ctgtccgttg cccgcggcgc gcgactgcgt
      841 taggctcgga acgtcgggcc gtccatcggt cgaggagctc tcgatgaaga ttcgcgccgc
      901 cgtattggag gcgttcggcg agccgttggt cgtgcaagag ctcgaccttg ccggtcccgc
      961 cgccggcgag gtgctcgtgc gcctggcggc ctgcggcgtg tgccacaccg atctgtacag
     1021 cgccagcggc gcggacccga gcggctatgc cccgtgcgtc ctcgggcacg agggcgccgg
     1081 cgtcgtcgag gcggtcggcg cgggcgtcaa cgggttgaga ccgggcgatc acgtgatcac
     1141 gctgttctcg cccgaatgcg gcgagtgcat ccactgcacg agcggcagga ccaacatctg
     1201 tctggcgatc cgcgagcagc aggggaaggg gttgctgccc gacgggacga cgcgactgtc
     1261 gcgcgacggc gaaccgatgc gccatttcat gggctgcagc accttcgccg aggcgacggt
     1321 gatgcccgag atcgcgctgg cgcggatcga cccccgcgcc ccgctcgatt ccgtctgcac
     1381 gttggcctgc ggcgcgacga ccggtctggc cggcgcgcta tggaaggccg aggtcgagcc
     1441 cggctcgacc tgcgcggtct tcggtgtcgg gatggtcggg ctgggcgccg tcgtcggctg
     1501 caggctcgcc ggggcggagc ggatcatcgc ggtcgatctc gcgccgaggc ggctcgagct
     1561 ggcgcgccgc tacggcgcga ccgagacgat cgtcgccggt gaggacagcg tccgtcgcat
     1621 cctcgatcgg accggaggct tcggcgcgga ctacaccttc gaggcgaccg gtctcgtcgg
     1681 tgtcatgcga caggcggtcg aggcgacccg catcggctgg gggctgtgca cggtgctcgg
     1741 cgtcgccggc aaaggcgaaa cgctcgacat cgtgccgcgg ctgttgatca ccggtcggcg
     1801 cgtgcagggt ggaagcttcg gcggggcgcg cggccgaacg cacgtaccgc agctggtcga
     1861 catgtatctc gcgggcaaga tcgacctgga cggattcgtc tcccaccggc tgacgctcga
     1921 cgaggtcaac cgcggattcg agctgatgcg ggcccaggac gggatccgtt cggtcgtcgt
     1981 tttctgattc ctcgcggcgg atgggagaac ttcgaacgtg atcatccaca agacgatgag
     2041 cgacggttgg ctctccaaca cctggctcgt cggcgacgaa ccgggcgggc atgccgtgct
     2101 gatcgacacg ggtggcccga tggacccgat cgtccgcgcg atcgaacagc atcgcctgac
     2161 ggtaacgcac gtgctctgca cgcaccacca catcgaccac gtgcagcaca actcggcgta
     2221 caagcgcagg ctcggctgcc cgatctgcgg tcatcactcg gagagcgagc tgttcggcgg
     2281 tctcgacctc gcgctcgacg acggcgacga gctggtgacg ggcggcctgc atctccgcgt
     2341 gctgcatacg cccggtcaca cgcggggcat gctcgccttc gtcgtcaacg acgagcgact
     2401 gttcaccggc gacacgttgt tccggcggac ggtcggcggg acgcgtgcgc cgggtcacac
     2461 cagcttcgag gatctgcacc actcgatcat ggacgtgctg atgaagttcc ccaagggaac
     2521 caagatccac ccgggtcaca ccgacgtctc gaccgtcggc gaggagtggg aggagaatcc
     2581 gttcattcgc atgtggcgcg gcgtggaccg gcccggcgag cggcgctgca ccgcgttcgg
     2641 cgagccggcg acactgttgc tccgcgcccg ggactacgac ggcggatcga agtgctgggt
     2701 gcgcttcgac gacggcggca agctcgatgt cgttccgggc tcgcaggttc gcaccgacga
     2761 cggcatgtaa aaagtcgcag atcccgaaac ccgagaacgc gcgccccgta tcccgccacg
     2821 tcggatttcg cgctctacga ggggtgaagc gcacggcgat ccggttgatg ccggtgtcgc
     2881 ccgaatccgg gcttcgagga ttcgatgctg cggctcggtt cacccctccg cgcgcggagc
     2941 gcggtcgttg cgccggcgat cggtctgtca ctggtagccg cttcctgcgg tgaaggccac
     3001 gctccggctc cggcctgtcc cgatctgagc tggaccggtc cgcgaccggg cacgagcctc
     3061 gttctcgtcg tcaacgatac gctgcgacgt gaccgcgtcg gtatctacgg cggccccgat
     3121 cagaccccga ccttcgacgc gttcgcgcgc ggcggcatgc tgttcgagcg ggcgacgagt
     3181 caggccccgt ggacctcgcc ggcgatcgcg agcctgttca gcggtctcta cccgtcggcc
     3241 cacggcgtgc gctcccatcc gtacctgcgc gcgacggccg acggcgcgac gacggcttcc
     3301 gccacgagga aggtcgacgt actgggcgac gacctgaccc tgctcgccga gctgctcgac
     3361 gacgccggct accacaccgc ggcgatcgtc ggcaacccgt ggctcgagag acgattcggc
     3421 ttcgcccagg ggttcgactt ctacgacgac tcgttcgccg actgggacgc accgggccgg
     3481 gacgtggccg gcgccgcgct cgactggctg gccggcgtgc ccgacggcga gccgtacttc
     3541 ctttacgtgc acacgatgga cacgcatcgg ccgtacccgt ccgtcagccg cgaagcactg
     3601 cacgctcgtc gtgacgagct ggtcgacgat cgtcgcccgc tcgatccggc cgtccgccag
     3661 tcgatcgccg atctcgtctt cctcgagggc gcgggcaagg cggtgcgggc cgggctgacg
     3721 ccgtcgctgt cgttgatcga gctggcctac gacgccggcg tgcgggcctt cgacgacagc
     3781 ttgtcggtgc tgctcgaggg gatggccggc gaggcggaag acgagacggc gctgatcgtc
     3841 ctgtcggacc acggcgaggc gctgttctcg cgaggttggg gcaaccacgg gcacggcctc
     3901 tacgaagacg aggtggcgat cccgatggcc gcgcggttgc ccggggttcg ggcggaacgg
     3961 gatcgggtgg gctgtccgac gggtctggtc gacgtgttgc cgacggtttg cagctacctc
     4021 gatctggact gcccggacga cctcggcggc gagagcttcc tcgcgccgcg caagcaggtc
     4081 ggttcgctgg ccgaccgccg cctgttggtc gagggcgtga tgcacaaacc gcagaaccgc
     4141 gcggtacgcg atcgtcgcta caagctgctg ctgcaaccgc agccggatct gtcggggacg
     4201 accgacgcgc tgttcgatac gtcgatcgac cctggggaaa cgaccaacct gttggagccc
     4261 ggctcgctga cgccgcgcca cgccgaggtg cgcgccgagc tcgagcgcgc ggcccgcgag
     4321 atgctgagcc gcagcgcgcc cgcgtcgaac gacgacgaga ccgttccact cgatgccgcg
     4381 accgagcggc gtctgcgttc gctgggctac atccgttagt tcgatcaccg cgtcttgacg
     4441 ggtcgccgcg ggggacacta gcttcgcacg cgatgacggg cgaacgacca acccgcggcg
     4501 agagctgggg cgtctggctg ctgttggcgg tggtctgcgg gatgacgctg cccgagctgg
     4561 ccgagccgcc cgcgaccggg ctcgacccgt cctggcgcgt cgggctccac ctggcacgcg
     4621 aacgcggcct gaccgtcggc gacgacctgt tcttcactta cggtccgtgg ggctacctca
     4681 ccgtgccact gacgatgacg cgaccactg
//
LOCUS       DASPGN010000581         2267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1632118, whole genome shotgun sequence.
ACCESSION   DASPGN010000581 DASPGN010000000
VERSION     DASPGN010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632118"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VD788_16695"
     CDS             <1..459
                     /locus_tag="VD788_16695"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HXV77950.1"
                     /translation="LARYGIPTLTDVLAAVPRDAFLDVELKGDPGDGIDAVVAVLEAG
                     RGAALERTAVSSFEPSALRAIARARPDWPRWLNAVDLSADTIALAAALGCVAVSVDWR
                     AITPATAAAVRAAGLEVAAWTVRRRATAARLDRLGVIAQCVEAAALEGPP"
     gene            456..1694
                     /locus_tag="VD788_16700"
     CDS             456..1694
                     /locus_tag="VD788_16700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXV77951.1"
                     /translation="MTERADLVVVGGGTIGGWAAWFTATSGARRVIVIERGLVGMGAS
                     SRAAGIVRAQGGTPETVALGRWSIDFYRRQAELIGTDSGFRELGYLILAVTEADERAG
                     RARVAMQRDAGLDVRWLSAAEAVHAIPTLAPEGHRGGSFFDGDGCIDPGRNVRAYSIA
                     LRSAGVEVRERTALTGLRTATGLDGGRQVVGVDTDDGPIETDRVILTGGPGLAEVGRL
                     AGIPIPVGATRHTVCVLEPHRDFDVDRMPMVFDIGEGLYWRLEEGGLLFGWSNPDEPP
                     GEARSIDWPYHDRIRARLEGLVPAVRGLGTRKIWAATIDYTPDHLPIIGPALGRDGGP
                     IAGVTIVSAGGHGMMWGPALARAAADLALDGRTDVVDLTNLGLDRFDSTGRSRIAPDP
                     VALPFPVETGGSDLGPQRSA"
     gene            complement(1691..>2267)
                     /locus_tag="VD788_16705"
     CDS             complement(1691..>2267)
                     /locus_tag="VD788_16705"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV77952.1"
                     /translation="LSARRVGPGGRAFGLDMTDDMLDLARRNAADAGATNVEFIKGTI
                     EAIPLAASSIDVVISNCVINLSADKGAVFGEIARVLRPGGRVGVSDVVADDILSADER
                     AERGSFAGCIAGALSFAEYRAGLEAVGLTGIEVTPTHAVADGLYGAIVRATKPADWTP
                     VAIREPSLPLPAPRLLAVVGEAGCCGGGGCC"
BASE COUNT          279 a          836 c          849 g          303 t
ORIGIN      
        1 ctcgcccgct acgggatccc gactctcacc gacgtcctcg ccgccgtccc gcgcgacgcc
       61 ttcctcgacg tggagctgaa gggcgacccg ggggacggaa tcgacgccgt cgtcgcggtc
      121 ctcgaggccg gtcgcggggc ggcgctcgag cggacggccg tctcgtcgtt cgagccgtcg
      181 gccctgcgcg cgatcgcccg cgcccggcct gactggccac gctggctcaa cgccgtcgac
      241 ctgtcggccg acacgatcgc cctcgccgcc gcgctcggct gcgtcgcggt cagcgtcgac
      301 tggcgcgcga tcacgccggc gaccgcggcg gccgtccgcg cggccgggct ggaggtcgcg
      361 gcctggaccg tgcgacggcg cgcgacggcg gctcgcctcg accgcctcgg cgtgatcgcg
      421 cagtgcgtcg aggcggccgc gctcgaggga ccgccatgac cgagcgagcc gacctcgtcg
      481 tcgtcggcgg ggggacgatc ggcggctggg cggcgtggtt cacggccacg agcggcgctc
      541 gccgggtgat cgtcatcgag cgcggcctcg tcgggatggg cgcgagctcg cgggcggccg
      601 gcatcgtccg tgcgcagggc gggacgccgg agaccgtcgc gctcggccgc tggtcgatcg
      661 acttctatcg ccgccaggcg gagctcatcg ggactgactc cggcttccgc gagctcggct
      721 acctgatcct cgccgtgacc gaggcggacg agcgcgccgg ccgcgcgcgg gtggcgatgc
      781 agcgcgacgc cggtctcgat gttcgctggc tcagcgcggc cgaggcggtt cacgcgatcc
      841 cgacgctcgc gccggagggc catcgcggcg gcagcttctt cgacggcgac ggctgcatcg
      901 accccgggcg caacgtccgg gcgtactcga tcgcgcttcg gtcggccggg gtcgaggttc
      961 gcgagcggac cgccctgacc gggctgcgga cggcgaccgg gctcgacggc gggcggcagg
     1021 tcgtgggcgt cgacacggat gacgggccga tcgagaccga tcgggtcatc ctgaccggcg
     1081 gtccgggcct cgccgaggtc ggccggctgg ccgggatccc cattccggtc ggggcgaccc
     1141 gccacaccgt gtgcgttctc gagccccacc gggacttcga cgtcgaccgg atgccgatgg
     1201 tgttcgatat cggcgaaggc ctctactggc gccttgagga gggcggcctg ctgttcggct
     1261 ggagcaaccc ggacgagccg cccggtgagg cacgctcgat cgattggccg tatcacgacc
     1321 ggatccgcgc ccggctcgag gggctcgtgc cggccgtccg tggcctcggc acccgcaaga
     1381 tctgggccgc cacgatcgac tacacgccgg atcacctgcc gatcatcggg cccgcgctcg
     1441 gccgggacgg aggcccgatc gcgggcgtga ccatcgtctc cgcgggcggc cacgggatga
     1501 tgtggggccc ggcgcttgcc cgggcggcgg ccgatctcgc cctcgatggg aggaccgacg
     1561 tggtggacct cacgaacctc ggcctcgacc ggttcgattc gactggccga agccggatcg
     1621 ccccggaccc ggtggcgctg ccgttccctg tcgaaaccgg cggctccgac ctgggcccgc
     1681 agcgcagcgc ctagcagcag cccccgccgc cgcagcaccc cgcctcgccg acgacagcga
     1741 gcagacgcgg ggccggtaac ggcagcgacg gctcgcggat tgcaaccggc gtccagtcgg
     1801 caggcttcgt cgcccggacg atcgccccgt acaggccgtc ggccaccgcg tgcgtaggcg
     1861 tgacctcgat cccggtcaga ccgacggcct cgagaccggc gcggtactcc gcgaacgaga
     1921 gcgcgccggc gatgcagccg gcgaagctgc cgcgctcggc ccgctcatcc gcggacagga
     1981 tgtcgtccgc cacgacgtcg ctcacgccga cgcggccgcc gggtcggagg acccgggcga
     2041 tttcgccgaa cacggcaccc ttgtcggccg acaggttgat gacgcagttg ctgatcacga
     2101 cgtcaatgct cgacgccgcg agcgggatcg cctcgatcgt gcccttgatg aactcgacgt
     2161 tcgtggcgcc ggcgtcggcc gcgttgcggc gcgcgaggtc gagcatgtcg tcggtcatgt
     2221 cgagcccgaa ggcgcgtccg ccgggaccga cgcggcgcgc cgacagg
//
LOCUS       DASPGN010000582         4647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_461609, whole genome shotgun sequence.
ACCESSION   DASPGN010000582 DASPGN010000000
VERSION     DASPGN010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461609"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VD788_16710"
     CDS             complement(<1..641)
                     /locus_tag="VD788_16710"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HXV77953.1"
                     /translation="MDTARKTGGWLLSAVLLLGVLPHVAAGDAGEDLLDLYAETYRFR
                     LGRPEVVRVTPDAAAVLFLRSGPRDPTRALFELDLASGRERTLATASDLLGGEAETLS
                     AEEQARRERQRVSARGIASYELSPDGRSVLVPLSGRLFLVDRADGSVRELAGSPVPAI
                     DARFSPDGSTVSCVRDGEIYLIGLADGVERPLTRGAGGGVTHGLAEFVAQEEMG"
     gene            764..1432
                     /locus_tag="VD788_16715"
     CDS             764..1432
                     /locus_tag="VD788_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77954.1"
                     /translation="MNSARLMRTLVVAAIVLAPAATAQQPDTTSMDDERFLVYLGSEA
                     AAASGAPDLDRKRLSKLLFSLGATLGSGKANVRMLQGAIELRGSAAASGRGLVAESYA
                     DYDRAVEDFRGGASAVLDRPESDPLLFRALTAGHEVCWRLDAYTRLVETYGVSMQDLV
                     AILASREACSQFRRAAFSVPVRRTLVAALEDAERQSTQVDDLREEIEALEQLVEDLRE
                     IERR"
     gene            1429..2559
                     /locus_tag="VD788_16720"
     CDS             1429..2559
                     /locus_tag="VD788_16720"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV77955.1"
                     /translation="MSRDGGGERRRQLLQWGTLVGAIVAVSIAHYSTPPSYFHWHVVY
                     QRLYYLPILFGAFWFGLRGGVALAIVTSVLYAPHIVLHWGHAPLYRSNQFAEIMMFFV
                     IGTIAGVLSDRIRSERERHRRTAEELARAYRRLQETFERLRLVDRLTALGTLSAGMAH
                     EIKNPLASIMGSIEILEAAVPAGDERQEFVRILRKEIQRLSGIVSSHLDLVRPANPER
                     EPQDVGEIARSVVELTRKQAIRQGVELTFEAEHRLPRAAVDGQQIQQAILNLVINAVQ
                     ALPDGGHVVVRVDRRDRRVRVVVEDDGPGLDDAALKQVFEPFFTTKEGGTGLGLSIAF
                     QIADQHGGDLRVEHRPGGGARFCLELPVVEQVAAPPAVGPNR"
     gene            2556..3920
                     /locus_tag="VD788_16725"
     CDS             2556..3920
                     /locus_tag="VD788_16725"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV77956.1"
                     /translation="MTAALRLLLVDDDESFRRVQEYQLAQAGYEVDSVADGQDALDRF
                     RDRLHELVVSDVRMPGLDGLGLLARIHAISADTPVVIITAQATVETAVEAMKRGAFDF
                     LTKPFPGEKLRLTLGRAREFARLRRENRQLRRQVEERLAFRNLIGSSPAMQRLFESME
                     LAAPTPSTVLILGETGTGKELVARAIHYNSPRKAGPFVTVNCGAIPDSLIEAELFGYR
                     RGAFTGADSDRKGRFETADGGTLLLDEVGEIPPALQPKILRVLQAGEVDRLGADRPIR
                     VDVRILAATHRDLEAMVQRGEFRQDLYYRLAVVPLTVPALRDRRDDIPLLAEHFLAKM
                     RVQAERPGLRLPPRAFALFDRYTWPGNVRELENTIERMVVLSRQDELSEETLPEKIRG
                     GAAGALATGFRLPPGGVRLEELERDLLRQALERHDGNRTKAARELGLTRNTLLYRMQK
                     YGLR"
     gene            3979..>4647
                     /locus_tag="VD788_16730"
     CDS             3979..>4647
                     /locus_tag="VD788_16730"
                     /inference="COORDINATES: protein motif:HMM:NF039593.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HXV77957.1"
                     /translation="MIYTCPMHPEIREPAPGSCPKCGMALEPVEVAADSEPNPEYADM
                     LRRLWVSLPLAVATFVLAMGEMIAGDPLARWLSPATRVYLQAASSTPVALWAAWPFYR
                     RALASVVRRSLNMFTLIGLGVALAYGYSLFATLAPGLLPPAFKNHAGAVPVYFEAAAV
                     ITVLVLLGQVLELKARSQTGEAIRRLLGLAAKRARRLRPDGTEEDVDLGDVRIGDRLR
                     VRPGE"
BASE COUNT          671 a         1615 c         1674 g          687 t
ORIGIN      
        1 cccatctcct cctgcgcgac gaactcggcc aagccgtggg tcacgccgcc gccggcgccg
       61 cgggtcagcg gccgctcgac gccgtcggcc agtccgatca ggtagatctc accgtcccgc
      121 acgcacgaga cggtcgatcc atcgggtgag aagcgggcgt cgatcgcggg caccggagaa
      181 ccggcgagct cgcgcaccga gccatcggcc cgatcgacca agaacagccg gcccgacagc
      241 gggacgagca ccgaccgacc gtcgggcgac agctcgtagc tcgcgatgcc gcgcgcgctc
      301 acgcgctggc gctcgcgccg ggcctgctcc tcggcgctca gcgtctcggc ctcgccgccg
      361 agcaggtcgg aggccgtggc cagcgttcgc tcgcgcccgc tcgcgaggtc gagctcgaac
      421 aacgcgcggg tgggatcgcg gggcccggaa cgcaggaaca gcacggccgc ggcatccggc
      481 gtgacgcgca cgacctcggg gcggccgagc cggaagcggt aggtctcggc gtagaggtcg
      541 agcagatcct cgccggcgtc gccggccgcg acgtgcggca gcacgccgag cagcagcacg
      601 gcggaaagga gccacccgcc ggtctttcgc gccgtatcca tcgccatccg acctccgctc
      661 gtcgcgcggc acgacgaaca tcttagccga gcggcgcggc gtccccgtat aatccccgcg
      721 tggagcggcg gcggcgcggc cgtcgggaag gaagggtcga ccggtgaact cggcgcgctt
      781 gatgcgaacg ctcgtcgtcg cggcgatcgt cctcgcgccc gcggccacgg cccagcaacc
      841 ggacaccacg tcgatggacg acgagcggtt cctggtctac ttgggatcgg aggcggccgc
      901 ggcgagcgga gcgcccgatc tcgatcgcaa gcgactgtcc aagctgctgt tcagcctcgg
      961 cgcgaccctc ggctcgggca aggccaacgt ccgcatgctc cagggcgcga tcgagctccg
     1021 cggctcggcg gccgcgtcgg ggcgcggcct cgtcgccgag agctacgccg attacgaccg
     1081 cgcggtcgag gacttccgcg gcggggcgtc ggcggttctc gatcgtccgg agtccgaccc
     1141 gttgctgttc cgggcgctga ccgcgggcca cgaggtgtgc tggcggctcg acgcctacac
     1201 ccgcctggtc gagacctacg gcgtttcgat gcaggacctg gtcgcgatcc tcgcctcgcg
     1261 cgaggcatgc agccagttcc gccgtgcggc gttcagcgtc ccggtgcgcc ggacgctggt
     1321 cgcggcgctc gaggacgcgg agcggcagag cacccaggtc gacgatctac gcgaggagat
     1381 cgaggcgctc gagcaactgg tcgaggacct gcgcgagatc gaacggcgtt gagccgggac
     1441 ggcggcggcg agcggcggcg ccagctgctc cagtggggga cgctggtcgg cgcgatcgtc
     1501 gcggtgagca tcgcccacta ctcgaccccg ccgtcctact ttcactggca cgtcgtctac
     1561 caacgactgt actacctgcc gatcctgttc ggtgccttct ggttcggtct gcgtggcggc
     1621 gtggcgctgg cgatcgtgac ctcggtgctg tacgcgccgc acatcgtcct gcactggggt
     1681 cacgcgccgc tgtaccgcag caatcagttc gccgagatca tgatgttctt cgtgatcggc
     1741 acgatcgccg gcgtgctgtc cgaccggatc cgcagcgagc gcgagcggca ccggcggacg
     1801 gccgaggagc tggcccgggc ctaccgccgg ctgcaggaga cgttcgagcg actgcggctg
     1861 gtcgaccgat tgaccgcgct cggcacgctg tcggcgggga tggcccacga gatcaagaac
     1921 ccgctggcgt cgatcatggg atcgatcgag attctcgagg ccgccgtacc ggccggcgac
     1981 gagcgccagg agttcgtccg catcctgcgc aaggagatcc agcggctgtc cgggatcgtc
     2041 tcgagccacc tcgacctggt gcggccggcc aaccccgagc gcgaaccgca ggatgtcggc
     2101 gagatcgccc gttccgtcgt cgagctgaca cgcaagcagg ccatccgtca gggcgtcgag
     2161 ctgacgttcg aggcggagca ccggcttccg cgcgcagcgg tcgacggcca gcagatccag
     2221 caggcgatcc tcaacctggt gatcaacgcc gtgcaggcgc tgcccgacgg tggtcacgtc
     2281 gtcgtgcggg tcgaccggcg tgaccgccga gtccgcgtcg tggtcgagga cgacggaccg
     2341 ggtctcgacg acgcggcgct caagcaggtc ttcgagccgt tcttcaccac caaagaaggc
     2401 ggcaccggcc tcggcctgtc gatcgcgttc cagatcgccg accagcacgg cggcgacctg
     2461 cgggtcgagc atcgtcccgg cggcggggcg cgcttctgcc tcgagctgcc ggtggtcgag
     2521 caagtggccg cgcccccggc ggtcgggccg aaccgatgac cgcagcgctc cggctgctgt
     2581 tggtcgacga cgacgagagc ttccgccgcg tgcaggagta tcagctggcg caggccggct
     2641 acgaggtcga cagcgtcgcc gacggccagg acgcgctcga tcggttccgc gaccgtttgc
     2701 acgagctggt cgtcagcgac gtgcgcatgc ccggcctcga cggcctcggg ttgctcgcgc
     2761 ggatccacgc catctcggcc gacacgccgg tcgtgatcat taccgcgcag gcgacggtcg
     2821 agaccgcggt cgaggcgatg aaacgaggcg cgttcgattt cctgactaaa ccgtttcccg
     2881 gcgagaagct gcggttgacc ctcggtcgcg cgcgcgagtt cgcccgattg cgccgcgaga
     2941 accgccagct ccgccgccag gtcgaggaac gcctcgcctt tcgcaacctg atcggctcga
     3001 gcccggcgat gcagcggctg ttcgaatcga tggagctcgc cgcgccgacc ccgtcgacgg
     3061 tgttgattct cggcgagacg ggaaccggta aggagctcgt cgctcgcgcg atccactaca
     3121 acagcccgcg caaggccggc ccgttcgtca cggtcaattg cggggcgatt cccgattcgt
     3181 tgatcgaggc cgagttgttc ggctaccgtc gcggggcgtt caccggagcg gacagcgacc
     3241 gcaagggcag attcgagacg gccgacggcg gcacgctgct gctggacgag gtgggcgaga
     3301 tcccgccggc gttgcagccg aagatcctgc gcgtgctgca ggcaggggag gtcgatcgtc
     3361 tcggcgccga tcggccgatc cgagtcgacg tgcggatcct ggcggcgacc catcgcgacc
     3421 tggaggcgat ggtgcagcgc ggggagttcc gccaggatct ctactaccgg ctggccgtcg
     3481 tgccgctgac cgtgccggcg ttgcgcgacc ggcgcgacga cattccgttg ttggccgagc
     3541 acttcctggc caagatgcgc gtgcaggccg agcggccggg actgcggctg ccgccgcgtg
     3601 cgttcgcgct gttcgatcgc tacacctggc ccggcaacgt ccgggagctc gagaacacga
     3661 tcgagcggat ggtcgtgctc agccgccagg acgagctgtc ggaggagacg ctgcccgaga
     3721 agatccgcgg cggagccgcc ggcgcgctcg ccaccgggtt cagactgcct cccggcggcg
     3781 tgcggctcga ggagctcgag cgcgacctgc tgcggcaggc gctcgagcgt cacgacggca
     3841 accgcaccaa ggcagcgcgc gagctcggtc tgacccgcaa cacgttgctc taccgcatgc
     3901 agaagtacgg cctacgctga tggtgcgtga ccggcggatc gccgaccgcg gcgaggtagt
     3961 gcaggagtcg tcgccctcgt gatctacacc tgcccgatgc accccgagat ccgcgaaccg
     4021 gctcccggga gctgtcccaa gtgcgggatg gcactcgagc cggtcgaggt cgccgcggat
     4081 tccgagccga acccggagta cgccgacatg ctgcggcggt tgtgggtctc gctgccgctg
     4141 gcggtcgcga ccttcgtgct cgcgatgggg gagatgatcg ccggcgatcc gctggcccgc
     4201 tggctgtcac cggccacgcg tgtctacctg caggccgcca gctccacgcc cgtcgcgttg
     4261 tgggctgcct ggccgttcta ccggcgcgcc ctcgcttccg tcgtccggcg cagcttgaac
     4321 atgttcacgc tgatcggcct cggcgtggcg ctggcgtacg ggtacagcct gttcgccacg
     4381 ctggcgccgg gtctcctgcc gccggcgttc aagaatcacg ctggcgccgt tccggtctat
     4441 ttcgaggcgg cggcggtcat caccgtgctc gtgctgctcg gccaggtgct ggagctgaag
     4501 gcccggagcc agacgggcga ggcgatccgc cggctgctcg gtctggcggc gaagcgggcg
     4561 agacgactcc ggccggacgg caccgaggag gatgtcgatc tcggcgacgt ccgcatcggg
     4621 gaccggctgc gcgtccgacc gggggag
//
LOCUS       DASPGN010000583        19169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_578242, whole genome shotgun sequence.
ACCESSION   DASPGN010000583 DASPGN010000000
VERSION     DASPGN010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 19169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19169
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578242"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1516
                     /locus_tag="VD788_16735"
     CDS             134..1516
                     /locus_tag="VD788_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77958.1"
                     /translation="MRDATTRGRDRSPLRLLGDPVPNTKYRVFTTKDIDRIAPLRGRS
                     KRLLDAMKAVSAVLPFRVNNYVIDELIDWDDIPNDPIFQLTFPQPGMLDPDDFERMYR
                     LVAAGVARDEVKRAAREIQHRLNPHPAGQMELNVPTLGGRRLEGSQHKYRETVLFFPA
                     PGQTCHAYCTYCFRWAQFVGIDELKFASRQADSLARYLRRHREVSSVLFTGGDPMVMK
                     ASVLRRYVEPLLDPALDHVASIRIGSKAPAYWPQRFVSDADADDVLRLFERVARAGRH
                     LALMAHYSHPRELSTKVSRAAVERIKSTGAVIRTQAPLIRHVNDSAAAWSEMWRRQVR
                     LGAVPYYMFVERNTGPKHYFEVPLARALEIFNAAIRTVSGIGRTVRGPSMSATPGKVL
                     VDGVAKIGREKVFVLKFLQGRDPDWVGRPFFARYDENASWLHELEPAFGEPEFFYEAG
                     LREIKAEALA"
     gene            complement(1523..2515)
                     /locus_tag="VD788_16740"
     CDS             complement(1523..2515)
                     /locus_tag="VD788_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXV77959.1"
                     /translation="MEYRRLGRAGLKLSELSYGSWVTFSFQLDASAAMDCIRTAYDAG
                     INFFDNAEVYAAGQSELLMGQALAKLGFARDSYCVSSKVYWGGDLPTQRGLHRKHVRD
                     ACHAALRRLQVDYLDLFFCHRPDLETPIEETVRAMDELVRQGKVLYWGTSEWSAEQIQ
                     QAHGTARQYGLVPPSMEQPEYNMFSRDRVEREFHRLYAEIGLGTTTWSPLASGILTGK
                     YGDGVPAGSRMSLEQYAWLKERVLDGDSGREKLDKAARLARVAREIGMTPAQLAIAWC
                     LENPNVSTVILGASTTAQLTENIGALDRRSALTDAVKTRIEEILDNRPELPRQY"
     gene            complement(2552..4987)
                     /locus_tag="VD788_16745"
     CDS             complement(2552..4987)
                     /locus_tag="VD788_16745"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXV77960.1"
                     /translation="MKPRTRKIVKRIMIALLLLVLAGVSAAGLTFVWVRSRLTASLPR
                     LDGELAVAGLTDTVVVERDADGVPTLRGRDRGDVSRALGFLHAQERFFQMDLLRRRAA
                     GELAELFGEAVVATDRDSRRHRFRARAEVALERAGPETRAWIAAYSDGVNAGLAALGE
                     APPEYFALRVEPRRWEPADTMLAVMAMYFDLQGYLWSRESDRGVMYDVLPHALADFLA
                     PLGTEWDAPIEGEVYTVPPIPGPDVYDLRKVAVAAGPPRLPPRRTLGRAARGSNSWAV
                     AAAHSRSGAAIVANDMHLGLGLPNVWYRVVMVFAESDGAERRLVGVTLPGVPALVVGS
                     NGDVAWGFTNSYGDWADLVILENDPQDANRYMTPDGSLAFRRHAERIAVKGADAVVLE
                     VLETIWGPVIDEDHLGRRRALRWVAHFPRATDLDFFDLERARNLEQALDVAARSGLPA
                     QNFVVGDRGGRIAWTIAGPIPARDRRDRTRPVPWSEAGRWDRWLEPPDYPRIVDPAAG
                     RVWTANNRVVAPPQLDAIGDGGFAHGARAGRIRSDLAAASPLDEAAMLAIQLDDRSPL
                     LDRWRELLLEVLDPRAVAEDARRGELRRIVERPWDGRAAVESASYRMVRGFRSFVATA
                     VFSAITAACLEADEHFDYDRTTNQSEGPLWRLVTERPSHLLSPEFADWRGQLLAAVDA
                     MLDYFAENHDGELDELTWGRRNTVRIGHPLSLAVPALSRWLDMPPTQLPGDVDVPRVQ
                     GTHFGASERLVVSPGREESGIFHMPGGQSGHPLSPFYRAGHAAWVQGSPSPLLPRETR
                     HRLQLSPADASAR"
     gene            complement(4991..5992)
                     /locus_tag="VD788_16750"
     CDS             complement(4991..5992)
                     /locus_tag="VD788_16750"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HXV77961.1"
                     /translation="MRVVEVGPTFGLDQLRVVERPTPRPGRGQVLLRVSAAALNYRDL
                     LMVRGLYNPRQPLPLVPCSDGVGEVVETGDAAERFAVGDRAIPIFAQDWLDGLPWPDL
                     LRSTLGGPLDGTLAELMVLDQRGLVPAPESLTDEECATLPCAGVTAWNAIAPLEAGGT
                     VLVEGTGGVALFALQLARARGARVIVTSSSDRKLERARELGAWETINYRDTPEWGKLA
                     RQLTGGAGVDLVVEVGGARTLPQALAAVAVGGQISLLGNLSGRTLELDLVPLFMRQIR
                     LQGLVVGSRASFEELVRALERHAVRPVIDRVFPMSNVREAMERMESATHFGKLCVRI"
     gene            6084..7358
                     /locus_tag="VD788_16755"
     CDS             6084..7358
                     /locus_tag="VD788_16755"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXV77962.1"
                     /translation="MSIYGNLRTMSLPDLLQWASVNKKTGVLELERNKICKRISFRDG
                     RIVACSSDDPPSRLGQFLLSRGKITKEQLRESLGRQEASGESLGKIFVAMGVLTEEEI
                     ESHVAAKAEENIYGLFDWTDAVFRFHERIGRDPFVIEVNLAVQDVVLKGVQRFDDLRR
                     IRDTFTSSGIVLRRTGKPVPAEVTSSPMARRVLDSVDGRRTLAEILLHAHASEFLVLK
                     FLYTLLRKGMVATTEVRDAHPNSRTLLDSAELPTAEAAAMLPAVSRVDRAPAGSDDEP
                     AVRDVEAALKQMARGNYPIALDVLNELYAAAPDDNHLRQLLLKAEAGYLDQARRREFS
                     PHRIPVPVPAAAANRGRLKPTELFLLSMLDGQSDIKSITWLAPLREVDVVKALQRMID
                     EGLIELREPSSPNAGSDTDDAQDVPSVQWSPV"
     gene            complement(7371..9167)
                     /locus_tag="VD788_16760"
     CDS             complement(7371..9167)
                     /locus_tag="VD788_16760"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXV77963.1"
                     /translation="MSNRILLVDDDSEAAERFRRAFAELGRELVSTSDADRVLDWFVD
                     VPPRLVLLAEGSPDIPTAAISRRLKADPRAEGVPVVVLTTDLPRDREELSRRIRILEC
                     DLIVARDVGCERLVEMVGRLIGDDGHPDDSRAGDRADAVRDDDTQPTLGALAKQINRD
                     SPSAESFRRALAEGAAIAAPAAAGDAGDRGSDIEAHLDTLFGGPRATATPPVRPPVAP
                     TGGPPGSVPPKMPPRPTADAPPRAPVASPARTAAGAGGSVALAGPPPGLSSPSIRRTT
                     IRQAPKAVEQLRQPRIRERQHQLETKRAGWIRPAAAGGGVILLVGTVGLGWWLLAGQD
                     GEPPAERPNERLARASLPPPAVERTAHEATPTTAEADSLPLPGPRADREPAVTPSPAQ
                     PPQPPRAAAQASRQATAPAPAPAPARSPPVAEATSDRESTAVSAPPAPTARQVPAAPV
                     VEPPTRTDGESESAGRPAASEPPTPPDAGATSVRPNEAQEPPVGPGDAAPTDSTEPPP
                     VTRTPAVVVERIEPQYPPKALRGIESTEIVLRVQIDESGRISRVLVDRGIPGSPIEAA
                     AVSTVLRWKFKPATENGVPTESWTIVRFAVEP"
     gene            complement(9271..11046)
                     /locus_tag="VD788_16765"
     CDS             complement(9271..11046)
                     /locus_tag="VD788_16765"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HXV77964.1"
                     /translation="MEYRSLYELFQGVSTTNSDRPAYRHKPRGSWVELTWADARALVN
                     RVSKALMALDVGQGQRVAILSRTRLEWSICDFGIVGCGGVTVGIYPSNLAEDCAYILE
                     HCDAEVIFVENGEQLEKIFDVRARLPRLRRIVVFDGAGDASRNVVGWDEFLAIGDAVE
                     DGALERRGRGLEPTDLASLVYTSGTTGVPKGAMITHENLIFTSWSAGQCLYLEPDFCT
                     LMFLPLAHIFARLISYCCMRNGNLVAFAEDITTVAADLKEIRPHFIASVPRIYEKVYD
                     KVISGAERAGGVKLKLFNFAIDAGTEASRLRQAKKPIPAGLKLRHAIADRLVLHKIRD
                     AFGGRLVWAVSGAAPLNPMIAEFFDACGVTILEGIGMTENTSFTNVNRLDNNKFGTVG
                     QVGPGIEQRIAEDGEVLYRGANLMKGYFKDEASTAETIDEQGWLKTGDIGELDDEGFL
                     RITDRKKDLIVTAGGKNVAPQRIERIMRTSRYIAQVVAHGDKRRYITALVTLDPENMS
                     QWAAEHGRSGAPIAELAQDAEVVALIEHEIAERNKQLASFESVKKFHILPRDFSIEEG
                     ELTPTLKIKRKVVFERYRGELDALY"
     gene            11119..11763
                     /locus_tag="VD788_16770"
     CDS             11119..11763
                     /locus_tag="VD788_16770"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HXV77965.1"
                     /translation="MASDPVSSLPAIDDKKSRMVEMKRCEWAGGDPLYQAYHDREWGI
                     PLHDDRELFEMLVLEGAQAGLSWLTILKKRDAYRRAFAGFDPAKVARFGRRDVARLLA
                     DPGIVRNRLKVAAAVDNARAVLEIQDEFGGFDPFIWRFVDGRQRVNAWKTLGQVPATT
                     PESDAMSKELKRRGMRFVGSTICYAYMQAVGMVNDHTTDCGWYRGASRRKRGRR"
     gene            11760..12212
                     /locus_tag="VD788_16775"
     CDS             11760..12212
                     /locus_tag="VD788_16775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXV77966.1"
                     /translation="MSLGQRVHEFVVETAGTGLVEITRSVVRWVGDSGVVDGLLTLHI
                     RHTSASLLIQENADPEVRRDLDRFFSRLVPYGDPIFRHTSEGPDDMPAHVRSALTASS
                     LSIPVVGGAPALGTWQGVYVWEHRARPHTRRVVAHLTGSWPESQSACM"
     gene            complement(12194..13825)
                     /gene="pruA"
                     /locus_tag="VD788_16780"
     CDS             complement(12194..13825)
                     /gene="pruA"
                     /locus_tag="VD788_16780"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR01236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HXV77967.1"
                     /translation="MSNGIFTVPVPTNEPVRDHAPGSPERREIEQWLDELAGRETEIP
                     ARIGGRRVRTGKIGKAVMPHDHAHVLATWHKCGKREVERAIKAAREAHRTWSKMSWHH
                     RASIFLKAADLLAGPYRPVLNAATMLGQSKTVHQAEIDSAAELIDFWRYNVEFMERIY
                     SEQPESAPGVWNYLEHRPLEGFVFAVTPFNFTSIAGNLPTAPAMMGNTVLWKPASSAV
                     YSAHFIMEILERAGLPPGVINMVPGSGSEVGDPVLASRELAGIHFTGSTDVFKAMWEA
                     IGRNIRSYDYYPRIVGETGGKDFVFAHRSADVPSLATALVRGAFEYQGQKCSAASRAF
                     IPRSIWPRVENRMLQMIARIKIGDPTDFQNFMGAVIDRAAFDSIKTYIDFARRSKDCT
                     ILAGGKCDARRGYFIEPTVVLAKRPDFRLLREEIFGPVLTVYVYDDASLDRTLELCDR
                     GSSYALTGAVFAQDRAAIVQITEALTHAAGNFYINDKPTGAVVGQQPFGGGRASGTND
                     KAGSYLNLIRWVSPRTIKENFVPPTDFPYPYMQAD"
     gene            complement(13920..14189)
                     /locus_tag="VD788_16785"
     CDS             complement(13920..14189)
                     /locus_tag="VD788_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77968.1"
                     /translation="MRRGSAVGMVVLLIALVVVMLLVARSWRELAPSAIELSRPRTGA
                     DPRAAHGDADAGGAPDDGLPGLDEARQHTDRHAAEVQDALDATNH"
     gene            14284..15180
                     /locus_tag="VD788_16790"
     CDS             14284..15180
                     /locus_tag="VD788_16790"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXV77969.1"
                     /translation="MSCSGRLVVLAVLAAIAGGGAVTGAPDDDVAAAVVDRLQIERRE
                     RGAPPLERRPGLDRVALERAREIAALPHSGRLSHAQPVGDQLRAAGIRWFSRAAAHVD
                     MVRGYLRPETGFLRSWRDHDTAWTNALASDFTTVGAATARSADGWIVLVIVLVVDLPY
                     PEDTGRLEQAVIELVNRVRDEHGLGRLAGRADLAVVARRHSEEMARRDFVAHVDPDGH
                     GTADRVLRAGLRFLQLGENIHMNRGARDPAAFAVERWLGSPGHRETMLDPAFVETGVG
                     AAIADDGRIFITQVFIAPAPTR"
     gene            15197..16162
                     /gene="gluQRS"
                     /locus_tag="VD788_16795"
     CDS             15197..16162
                     /gene="gluQRS"
                     /locus_tag="VD788_16795"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HXV77970.1"
                     /translation="MSRPRARYAPSPTGDLHLGNASSALLAWLSVRAAAGRFVVRVED
                     LDRARSRQELVPRILDDLAWLGLDWDEGPDRGGPHAPYEQWRRLERYEAALAELQRAD
                     RVYPCFCSRRDVAAAASAPQLPGDEVRYPGTCRELDSARARRRIAAGEPHALRFRVPR
                     GARVEFDDIVHGRVVDPAGTPTGDFVVRRADGVPAYQLAVVVDDAAMKITEVVRGDDL
                     LPSTARQLLLFEALGVAPPRFGHVPLLLGPDGVRLAKRHRGSSVGELRERGLDAGKLV
                     GRLAERLGLTANGDPVRPSELVSGFSLARLRAAPGGILIDPTGWS"
     gene            complement(16172..17569)
                     /locus_tag="VD788_16800"
     CDS             complement(16172..17569)
                     /locus_tag="VD788_16800"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXV77971.1"
                     /translation="MHTPPVRWTLLALCACSYAGSIRAADDPIAARIVEIAKEESRAY
                     DKLAWLTDRIGHRLSGSAALDRAVEWAAAEFERDGLPRVWTDVVAVPHWVRGEESAQI
                     VVPARHDLAILGLGGTIGTPDDGVTGEVVVVDGFEALETLGEGARGKIVLFDRAMERG
                     FDTDNGYDALSKLRSRGASAAAKQGAIAMLIRSLGTADYRLPHTGALRYEDDVAPIPA
                     AAVSGEDAMLISRLTRQGETVRVRLRLGARMLPDAVSANVLAELPGRERPEQIVVIGA
                     HLDSWDVGTGAHDDGAGCAIVMETMRLLKQHDLIPKRTIRAVLFTNEENGLRGARDYA
                     ERYVDQMTNHVAAIESDLGGDAPIGFGVTAGAGGADIVGEIAAQLVSIGADRVIPRGG
                     GADISLMKKFGVPQIGQLPDPTRYFDYHHTEADTLDKVARDKLDLNVAAMAVMAYMLA
                     ERDEPLPRIPQDERD"
     gene            17623..18879
                     /locus_tag="VD788_16805"
     CDS             17623..18879
                     /locus_tag="VD788_16805"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HXV77972.1"
                     /translation="MIVKPFRAWRPPAELAAVVASPPYDVLSSAEARRLAAGEPRSFL
                     HVTKPEIDLDPATDPYADAVYATGARNFRAMIERGWLVRDERASYYVYRLTMDGRAQT
                     GLLGAAGVDDYRTGRIKRHEHTRPEKEKDRVRLNDAIGAHPGPVFLAYRGLPELNAIV
                     NGVVADGPRTDFAADDGVRHEVWVVDEPATCRKIETLFRHVPATYVADGHHRAAAAVK
                     VAELRDSRQPPGASADRESAWRFFMAAHFPYRQLRVLDYNRVVRDLNGMEPATFLDRL
                     ARRGFHVGADHRKRRPPSPETFGMYLDRRWYLLTPRPELIPTDVVGKLDVAILNDQVL
                     SPLLGIGDPRTDRRVEFVGGIRGMDELERLVDSGDYEVAFAVHPTSLEHVMAVADAGR
                     VLPPKSTWFEPKLRSGLIVQSFDEGL"
     gene            complement(18889..>19169)
                     /locus_tag="VD788_16810"
     CDS             complement(18889..>19169)
                     /locus_tag="VD788_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77973.1"
                     /translation="PTTNLPEIHSLLRRTDLLPATLESIASNPRFNHDEQVRIAVATH
                     PRVTFPTADKIVAQMNEITLDKLIRRPGLQPGVKQKLMAKLSRKHRGG"
BASE COUNT         2884 a         6675 c         6590 g         3020 t
ORIGIN      
        1 cggtatcgac cgcgtcgcgc gcatggaatt caaattcctt aatcgcgctt cgatcggaac
       61 cggagaaccc gcggccgtgg gatggcttgg acaggctccc cgcccaacgg ccgcatcgac
      121 cagggggaac ctgatgcgtg acgccacgac gcgcggccgc gaccggtcgc cgctgcgact
      181 gctgggcgat ccggtaccga acaccaagta ccgggtcttc acgaccaagg acatcgaccg
      241 catcgcgccg ctacgcggac ggagcaagcg gcttctcgac gcgatgaaag cggtttcggc
      301 ggtgttgccg ttccgcgtca acaactacgt catcgacgag ctgatcgact gggacgacat
      361 cccgaacgac ccgatcttcc agctgacctt tccacagccg ggaatgctcg acccggacga
      421 cttcgagcgg atgtaccgcc tggtcgccgc cggcgtcgcc cgcgacgagg tgaagcgcgc
      481 cgcgcgcgag atccagcacc ggctgaaccc ccatcccgcc ggccagatgg agctcaacgt
      541 cccgacgctc ggcggcaggc ggctcgaggg ttcccagcac aagtaccgcg aaaccgtgct
      601 gttcttcccc gcgcccgggc agacctgcca tgcgtattgc acctactgct tccgctgggc
      661 ccagttcgtc ggcatcgacg agctgaagtt cgccagccgc caagccgaca gcctggcgcg
      721 ttatctccgg cgccaccgcg aagtgagcag cgtgttgttt accggcggcg acccgatggt
      781 gatgaaggcc tcggtactgc gccgctacgt cgaaccgctg ctcgaccccg cgctcgacca
      841 cgtcgcgagc atccgcatcg gcagcaaggc gccggcctac tggccgcagc ggttcgtcag
      901 cgacgccgac gccgacgacg tgctacgcct gttcgagcgg gtggcccgcg ccggtcgcca
      961 cctggcgctg atggcgcact acagccatcc gcgcgagctg tcgaccaagg tttcacgtgc
     1021 ggcggtcgag cggatcaaga gcacgggcgc cgtcatccgg acgcaggcgc cgttgatccg
     1081 ccacgtcaac gactcggccg cggcctggag cgagatgtgg aggcggcagg tccgtctcgg
     1141 cgcggttccc tactacatgt tcgtcgagcg caacaccggt cccaaacact acttcgaggt
     1201 cccgctggcc cgcgcgctcg agatcttcaa cgccgccatt cgcaccgttt ccggcatcgg
     1261 tcgcaccgtc cgcggaccgt cgatgtcggc gacgcccggc aaggtgctgg tcgacggcgt
     1321 cgcgaagatc ggcagggaaa aggtcttcgt gctgaagttc ctccagggtc gggatccgga
     1381 ttgggtcggc cggccgttct tcgcgcgtta cgacgagaac gcgtcgtggc tgcacgaact
     1441 cgagccggcc ttcggcgagc ccgagttttt ctacgaggct gggctgcgcg agatcaaggc
     1501 cgaagcgctc gcctgacccg cgtcagtact gccgaggcag ctccggccgg ttgtcgagga
     1561 tctcctcgat tcgcgtcttg acagcgtcgg tcagcgccga gcgtcggtcg agcgcgccga
     1621 tgttctcggt cagttgcgcc gtcgtcgacg ccccgaggat caccgtgctg acgttcgggt
     1681 tctcgaggca ccaagcgatg gccagctgcg ccggggtcat gccgatctcg cgtgccacac
     1741 gcgcgagtcg cgccgccttg tcgagcttct ctcggccgga atcgccgtcg agcacccgct
     1801 ccttgagcca ggcgtattgc tcgagcgaca tccgcgagcc ggcgggcacc ccgtcgccgt
     1861 actttccggt caggattccc gatgccagcg gcgaccaggt ggtcgtgccc agaccgatct
     1921 ccgcgtagag ccggtggaac tcgcgctcga cgcggtcgcg actgaacatg ttgtactccg
     1981 gctgctccat cgacggtgga acgagaccgt actgccgcgc ggtgccgtgg gcctgctgga
     2041 tctgctccgc gctccactcg gacgtgcccc agtacagcac cttcccctgg cgcacgagct
     2101 cgtccatcgc gcgcaccgtc tcctcgatcg gcgtctcgag atcgggtcga tggcagaaga
     2161 acagatcgag gtagtccacc tgcagccgtc gcagcgccgc gtggcaagcg tcgcggacgt
     2221 gcttgcggtg caagccgcgc tgcgtcggca ggtctccgcc ccagtagact ttgctcgata
     2281 cgcagtagct gtcgcgggcg aagccgagct tggccagcgc ctggcccatc agcagctcgg
     2341 actgcccggc ggcgtagacc tcggcgttgt cgaagaagtt gatcccggca tcgtaggccg
     2401 tgcgaatgca gtccatcgcc gccgacgcgt cgagctggaa cgagaaggtg acccacgacc
     2461 cgtaggacag ctcggacagt ttcaggccgg cacgacccag ccgccgatat tccatttcgc
     2521 gtgctccggt cgaacggccg attctagcag gtcagcgcgc cgaggcgtcg gccggagaca
     2581 gctgcaaccg gtggcgcgtc tcacgcggca gcagcggcga cgggctaccc tgcacccacg
     2641 cggcgtgtcc cgcgcggtag aacggcgaca gcgggtgacc gctttggcca ccgggcatgt
     2701 ggaagatgcc gctctcctcg cggcccggtg agacgaccag ccgctcggac gcgccgaagt
     2761 gcgttccctg aacgcgagga acgtcgacgt cacccggaag ctgggtcggc ggcatgtcga
     2821 gccagcgcga caacgccggc acggcgaggc tcagcggatg gccgatgcgc accgtgttgc
     2881 gccggcccca ggtcagctcg tccagctcgc cgtcgtgatt ctccgcgaag tagtcgagca
     2941 tcgcgtcgac cgcggcgagc aactgtcccc gccaatctgc gaactccggg ctcaacaggt
     3001 gtgacggtcg ctcggtgacg agtcgccaca gcggaccctc ggattggttc gtcgtgcgat
     3061 cgtaatcgaa gtgctcatcg gcctcgagac aggcggcggt gatcgccgag aaaacggccg
     3121 tcgcgacgaa cgatcgaaag ccgcggacca tgcggtacga ggccgactcg accgccgccc
     3181 gcccgtccca cggtcgttcg acgatgcgac gcaactcgcc gcgacgcgcg tcttcggcca
     3241 ccgctcgcgg gtcgagcacc tcgagcagca actcgcgcca gcggtcgagc aacggcgagc
     3301 ggtcatcgag ttggatggcg agcatcgcag cctcgtcgag cgggctcgcg gcggcgagat
     3361 cgctccgtat ccttccggcg cgcgcgccgt gcgcgaatcc gccgtcgccg atcgcatcga
     3421 gctgcggcgg ggcgacgacg cggttgttgg cggtccacac ccggcccgcg gccgggtcga
     3481 cgatacgcgg ataatcgggt ggctcgagcc agcgatccca gcggccggcc tcgctccaag
     3541 gaaccgggcg agtccggtcg cggcggtccc gcgcggggat cggcccggcg atcgtccacg
     3601 cgatccgacc gccccggtcg ccgacgacga agttctgcgc cggtagaccg gaccgggccg
     3661 cgacgtcgag cgcctgctcg aggttgcgcg cccgctcgag gtcgaaaaaa tcgaggtcgg
     3721 tcgcgcgcgg gaagtgcgcc acccagcgca acgcgcgacg ccgccccagg tggtcctcgt
     3781 cgatcaccgg tccccagatc gtctcgagga cctcgagcac gacggcgtcg gcgcccttga
     3841 ccgcgatccg ctcggcgtgg cggcggaagg cgagcgatcc gtccggcgtc atgtatcgat
     3901 tcgcgtcctg cgggtcgttc tccagaatca ccagatcggc ccaatcgccg tagctgttgg
     3961 tgaagcccca ggcaacgtct ccgttgctgc cgaccacgag cgccggcacg cccggcaagg
     4021 tcacgccgac gagcctgcgc tcggcgccgt cgctctccgc gaacaccatc acgacgcgat
     4081 accacacgtt gggcaggccg aggccgaggt gcatgtcgtt cgccacgatc gcggcgcccg
     4141 atcgggaatg tgcggcggcg acggcccagc tgttgctgcc gcgcgccgcc cgcccgagcg
     4201 tgcgacgcgg cgggaggcga ggcggaccgg cggcgacggc gaccttccgc aggtcataga
     4261 cgtcgggacc cgggatcggc ggcacggtat agacctcgcc ttcgatcggg gcgtcccatt
     4321 ccgtgccgag cggtgcgagg aagtcggcga gcgcgtgggg caggacgtcg tacatcacgc
     4381 cgcggtcgga ttcgcgcgac cacaggtagc cctgcaagtc gaagtacatc gccatcaccg
     4441 ccagcatcgt gtcggccggc tcccagcgtc gcggctcgac ccgtagcgcg aagtattcgg
     4501 gcggcgcttc gccgagcgcg gccagaccgg cgttgacgcc gtcggagtag gcggcgatcc
     4561 aggcccgcgt ctcgggcccc gcccgctcga gcgcgacctc ggccctcgcg cggaagcggt
     4621 gcctgcgcga gtcccggtcg gtcgcgacga cagcctcccc gaacagctcg gccagctctc
     4681 ccgcggccct acggcgcagc aggtccatct ggaagaaccg ctcctgcgcg tggaggaatc
     4741 cgagcgcgcg actgacgtcg ccccggtccc gcccgcgaag cgtgggcacg ccgtcggcgt
     4801 cgcgctcgac cacgaccgtg tcggtcaggc ccgcgaccgc gagctcgccg tcgagccggg
     4861 ggaggctggc cgtgagacgc gagcggaccc agacgaaggt caacccagct gccgacacgc
     4921 cggcgagcac cagcagcagc agcgcgatca tgatcctctt gacgatcttg cgggttcgcg
     4981 gcttcacgcg tcagatcctc acgcagagct tgccgaagtg tgtcgccgac tccatccgct
     5041 ccattgcctc gcggacattc gacatcggga agacccggtc gatcaccggt cgcacggcgt
     5101 gccgctcgag cgcccggacc aactcctcga acgacgcgcg gctgccgacg acgagcccct
     5161 gcagccgaat ctgacgcatg aagagcggaa ccagatcgag ctcgagggtt cgtccgctga
     5221 gattgcccaa caggctgatc tgtccgccga cggcgaccgc ggccagggct tgtggcagcg
     5281 tccgcgcgcc gcccacctcg acgacgagat cgaccccggc gccgccggtc agctgccgcg
     5341 cgagcttgcc ccattccggc gtgtcgcggt agttgatcgt ctcccaggcg ccgagctcgc
     5401 gcgcccgttc gagcttccga tccgagctcg acgtgacgat cacccgggcg ccccgcgccc
     5461 gcgcgagctg gagcgcgaac agcgccacgc cgcccgtccc ctcgaccaag acggtgccgc
     5521 cggcctcgag cggcgcgatc gcgttccagg ccgtgacgcc ggcgcaaggc agcgtcgcgc
     5581 actcttcatc cgtgagggac tccggagcgg ggacgagccc tcgctggtcg agcaccatca
     5641 gctcggccag ggtcccgtcg agcggcccgc ccaaggtcga ccgaaggaga tccggccacg
     5701 ggaggccgtc gagccagtct tgggcgaaga tcgggatcgc acggtccccg acggcgaacc
     5761 gctcggcggc gtcgcctgtt tcgaccacct cgccgacgcc gtccgagcag gggacgagcg
     5821 gcagcggctg ccgcgggttg tacaggccgc ggaccatgag caagtcccgg taattcagtg
     5881 cggccgccga tacccggagc agcacctggc ctcggcccgg ccgcggggtc ggtcgctcga
     5941 cgacgcgcaa ctgatcgagc ccgaaggtcg ggccgacctc gacgactctc acgacgagtc
     6001 tccctcccct cttgccgcca cggacttgac ccacggccgt acgaaactac gtttgcctat
     6061 gatccgtcgg ccgaggagtc tccatgtcga tctacggcaa ccttcgcacc atgtcgctac
     6121 cggacctgct ccagtgggcg agtgtaaaca agaagaccgg ggtcttggaa ctcgaacgaa
     6181 acaagatctg taaaagaatc tcgtttcggg acggtcgcat cgtcgcctgc tcgtccgatg
     6241 acccgccctc gcggctcggc cagttcctgc tctcccgagg gaagatcacc aaggagcagc
     6301 tgcgcgagtc gctcggccga caggaagcca gtggcgaaag cctgggaaaa atcttcgtcg
     6361 cgatgggggt gctcaccgag gaggagatcg aatcccacgt cgcggccaag gccgaggaga
     6421 acatctacgg gctgttcgat tggactgacg ccgtcttccg cttccacgaa aggatcggca
     6481 gggacccgtt cgtgatcgag gtcaatctcg ccgtccagga cgtggtgctc aagggcgtcc
     6541 aacgcttcga tgacctccga cggatccgcg acaccttcac cagctcgggg atcgtgcttc
     6601 ggcgcaccgg taagccggtg ccggccgagg tgaccagcag tccgatggct cgtcgggtgc
     6661 tcgactcggt cgacgggcga cgtacgctcg ccgagatcct gttgcacgcc cacgcctccg
     6721 agtttctcgt gctgaagttc ctttacacgc tgttgcgcaa ggggatggtc gcgaccacgg
     6781 aggtacgcga cgcacacccc aactcgcgca cgctgctcga ttcggcggag ctgccgacgg
     6841 ccgaggcggc cgcgatgcta cccgcggtct cgcgtgtcga tcgggcgcca gccggcagtg
     6901 acgacgagcc ggcggtgcgc gacgtcgaag cggcgctcaa gcagatggcg cgcgggaact
     6961 acccgatcgc gctcgacgtc ctcaacgaac tgtacgccgc cgctccggac gacaatcacc
     7021 tgcgccagct gctgttgaag gccgaggcag gctatctcga ccaggcccgg cgacgagagt
     7081 tctccccgca caggatcccc gtcccggtgc cggcggcggc ggccaaccgc ggccgtctca
     7141 agccgaccga gctcttcttg ctgtcgatgc tcgacggaca gtcggacatc aagtcgatca
     7201 cctggctcgc gccgttgcgc gaggtcgatg tggtcaaggc gctacagcgg atgatcgacg
     7261 agggactgat cgagcttcgc gagccgagct ccccgaacgc cggctccgac accgacgacg
     7321 cgcaggacgt gccgagcgtg cagtggagcc ccgtctgagc cgtgggacgc ttacggctcg
     7381 accgcgaacc gcacgatcgt ccaggactcg gtcgggactc cgttctcggt cgccggcttg
     7441 aacttccaac gtaggacggt gctgacggcc gcggcctcga tcggggagcc gggaatcccc
     7501 cggtcgacga gcacgcgcga gatacgcccg ctctcgtcga tctggacgcg cagcacgatt
     7561 tccgtcgact cgatcccgcg cagcgccttg ggcgggtact gcggttcgat gcgctcgacg
     7621 acgaccgcgg gggtgcgggt caccggaggt ggctcggtcg agtcggtcgg cgccgcatcg
     7681 ccaggcccga cgggcggttc ctgtgcctcg ttcggccgga ccgaggtcgc gccggcatcc
     7741 ggcggcgtgg gcggctcgga cgcggccggt cgccctgccg actccgattc gccgtcggtc
     7801 ctggtcggcg gttcgacgac cggggcggcg ggcacctggc gagcggtcgg ggcggggggc
     7861 gcgctcaccg ccgtcgattc tcgatccgac gtcgcttcgg cgacgggcgg cgaccgagcc
     7921 ggagccggtg ccggtgccgg tgctgtggcc tgccgggaag cctgcgcggc ggcacgcggc
     7981 ggttgcgggg gctgcgccgg cgagggggtc acggccggct cccggtccgc acgcggcccg
     8041 ggcagcggga gcgagtcggc ctcggcggtc gtgggggtcg cctcgtgggc cgtccgctcg
     8101 acggcgggag ggggcagcga cgcccgcgcg agccgttcgt tcggtctctc ggccggcggt
     8161 tcgccgtcct ggccggcgag aagccaccat cccaggccga ccgtgccgac gagcaagatc
     8221 acgccgccgc ccgcggcggc cggccggatc caccctgcgc gcttggtctc gagctggtgc
     8281 tgccgttcgc ggatccgcgg ctgacgcagc tgctcgacgg ccttcggggc ctgacggatc
     8341 gtggtacgcc ggatcgatgg cgaggagaga ccgggaggtg ggccggccag ggcaacgcta
     8401 ccgccggcgc cggcagccgt ccgggccggt gacgcgacgg gcgctcgagg cggtgcgtcg
     8461 gcggtcggtc gtggcggcat cttgggtggg accgacccgg gtggccctcc cgttggcgcg
     8521 accggtggtc gcacaggcgg cgtggcggtt gccctcggcc cgccgaacag cgtatcgagg
     8581 tgcgcttcga tgtccgagcc gcggtcaccc gcatcgcccg ccgccgccgg cgcggcgatc
     8641 gcggctccct cggcgagcgc ccgccgaaac gactcggccg acgggctgtc ccggttgatc
     8701 tgcttcgcca gtgcgccgag cgtcggttgc gtatcgtcgt cgcggaccgc gtcggctcgg
     8761 tcgccggcgc gggagtcgtc ggggtggcca tcgtcgccga tcagccggcc gaccatctcg
     8821 accagacgtt cgcagcccac gtcgcgggcg acgatcaggt cgcattcgag gatgcggata
     8881 cgccgggaca gttcctcgcg atccctcgga aggtcggtcg tcagcaccac caccggcacg
     8941 ccctcggccc gcggatccgc cttcaaccgt cgcgagatcg ccgccgtcgg gatgtcgggc
     9001 gagccctccg ccagcagcac cagccgcggc ggcacgtcga cgaaccagtc gagcacgcgg
     9061 tccgcgtcgc tcgtcgatac gagctcccga ccgagctccg cgaaggcgcg acggaatcgc
     9121 tcggcagcct cggagtcgtc gtcgaccagc aggatccggt tgctcacggc ggacgcccac
     9181 cccaggggcc gcccgatgcg gcccgatgcc cagcaaacat aggtccctgc tcccagcgct
     9241 tcaaggccgg gttcgacaag cacccgtgtg tcagtagagc gcgtccagct cgccgcggta
     9301 gcgctcgaac accaccttgc gtttgatctt cagcgtgggc gtcaactcgc cctcctcgat
     9361 cgagaaatcc cgtggcagga tgtggaactt cttgaccgat tcgaacgacg cgagctgttt
     9421 gttgcgctcg gcgatctcgt gctcgatcag cgcgacgacc tcggcgtcct gggccagctc
     9481 ggcgatcggc gcgcccgacc gaccgtgctc ggcggcccac tgcgacatgt tctccggatc
     9541 gagcgtgacc agcgccgtga tgtagcggcg cttgtcaccg tgcgcgacga cctgcgcgat
     9601 gtaacgcgag gtgcgcatga tgcgctcgat tcgttgcggg gcgacgttct tgccgccggc
     9661 ggtgacgatc aggtccttct tgcggtcggt gatgcggagg aacccttcgt cgtccagttc
     9721 gccgatgtcg ccggtcttga gccagccttg ctcgtcgatc gtctcggcgg tcgaggcctc
     9781 gtccttgaag tagcccttca tcaagttcgc tccgcgatag aggacctcgc cgtcctcggc
     9841 gatccgctgc tcgattcccg ggccgacctg gccgaccgtg ccgaacttgt tgttgtccag
     9901 ccgattgacg ttggtgaacg acgtattctc cgtcatcccg atgccctcga gaatcgtcac
     9961 gccacaggcg tcgaagaact cggcgatcat cgggttcaac ggggccgcac cggagaccgc
    10021 ccacaccaac cgaccgccga acgcgtcgcg aatcttgtgc agcaccagcc gatcggcaat
    10081 cgcgtggcgc agcttgagcc cggcgggaat cggtttcttg gcctgtctca accggctcgc
    10141 ctcggtcccg gcatcgatcg cgaagttgaa cagcttgagc ttgaccccgc cggcccgctc
    10201 ggcgccggag atcaccttgt cgtagacctt ctcgtagatt ctcggcacgc tcgcgatgaa
    10261 atgcgggcgg atctccttca ggtcggcggc gaccgtcgtg atgtcctcgg cgaacgccac
    10321 gaggttgccg ttccgcatgc agcaatagga gatcagccgc gcgaagatgt gcgccagcgg
    10381 caggaacatc agcgtgcaga aatccggctc gaggtacagg cactgtccgg ccgaccagct
    10441 ggtgaagatc aggttctcgt gcgtgatcat cgcacccttg ggcacaccgg tggtccccga
    10501 ggtgtagacc aaggacgcca ggtcggtggg ttcgaggccc cgcccgcgcc gctcgagcgc
    10561 gccgtcctcg accgcgtcgc cgatcgcgag aaactcgtcc caaccgacga cgttccgcga
    10621 cgcgtcgccg gccccgtcga aaacgacgat gcggcgcagg cgcggcaacc gcgcgcgcac
    10681 gtcgaagatc ttttccagtt gctcgccgtt ctcgacgaag atcacctcgg cgtcgcagtg
    10741 ctcgaggatg taggcgcagt cctcggccag gttcgacggg tagattccga ccgtcacgcc
    10801 gccgcagcca acgataccga aatcgcagat cgaccactcg aggcgggtgc gcgacaggat
    10861 cgccacacgc tggccctgcc cgacgtcgag cgccatcagc gccttcgaca cgcggttcac
    10921 cagggcgcgg gcatcggccc aggtgagctc gacccacgaa ccgcgtggct tgtggcggta
    10981 ggccggccga tccgagttgg tggtcgacac tccctggaac agctcgtaca gcgaacgata
    11041 ctccatggcg gttttctccg atgccacgaa cggggtggcg gggctcggca gggaaaccct
    11101 agcgccgggg acctcgaaat ggcaagcgat cccgtctcgt cgctgccggc gatcgatgat
    11161 aaaaagagcc gcatggtcga gatgaagcgc tgcgagtggg cgggcgggga cccgctgtac
    11221 caggcctacc acgaccgcga gtgggggatc ccgctgcacg acgaccggga gctgttcgag
    11281 atgttggtgc tggaaggggc gcaggccggg ttgagctggc tgacgatcct gaagaagcgc
    11341 gacgcctatc ggcgcgcatt cgccggcttc gacccggcca aggttgctcg cttcggccgt
    11401 cgcgatgtcg cccgattgct ggccgacccg ggaatcgtcc gcaaccggct caaggtcgcg
    11461 gccgcggtcg acaacgcccg ggccgtgctc gagatccagg acgagttcgg cggcttcgac
    11521 ccgttcatct ggcggttcgt cgacggacgc cagcgggtca acgcctggaa gacgctcggg
    11581 caggtgccgg cgacgactcc cgagtcggac gcgatgagca aggaactgaa gcgccgcggt
    11641 atgcgcttcg tcggctcgac gatctgctat gcctacatgc aggcggtcgg aatggtcaac
    11701 gaccacacga ccgactgcgg ctggtaccgc ggcgcctcgc ggcgaaagcg cggtcggcgg
    11761 tgagcctcgg gcagcgcgtc cacgagttcg tcgtcgagac tgccggcacg ggtctggtcg
    11821 agatcacccg gtcggtcgtg cgctgggtcg gcgacagcgg cgtcgtcgac gggttgttga
    11881 ccctgcacat ccgccacacg tcggcctcgc tgttgatcca ggaaaacgcc gaccccgagg
    11941 tccgacgcga cctcgatcgg ttcttctcgc ggttggtgcc ctacggcgat ccgatctttc
    12001 gtcacacgag cgagggacca gacgacatgc cggcgcatgt gcgttcggcg ctgacggcca
    12061 gcagcctgtc gatcccggta gtgggcggcg cgccggcgct cggcacctgg cagggcgttt
    12121 acgtctggga gcaccgcgcc cggccgcaca cgcgccgcgt cgtggcccac ctgacgggga
    12181 gctggccgga atctcagtcg gcctgcatgt aagggtaggg gaagtcggtc ggcgggacga
    12241 agttctcctt gatcgttcgc gggctgaccc aacggatcag attgaggtag ctgcccgcct
    12301 tgtcgttggt tcccgacgcc cggccgcccc cgaacggttg ttgtccgacg acggcgccgg
    12361 tgggcttgtc gttgatgtag aagttcccgg ccgcgtgggt cagtgcctcg gtgatctgga
    12421 cgatcgccgc tcggtcctgc gcgaagaccg cccccgtcag cgcgtagctc gatccccgat
    12481 cgcacagctc gagcgtgcgg tccaggctgg cgtcgtcgta gacgtagacc gtcagcaccg
    12541 ggccgaagat ctcctcgcgc aacagccgga aatcgggcct cttcgccagc accacggtcg
    12601 gctcgatgaa atagccgcgc cgagcgtcgc atttcccacc ggccaggatc gtgcagtctt
    12661 tcgaacgtct ggcgaagtcg atgtaggtct tgatcgagtc gaacgcggcg cggtcgatca
    12721 ccgcccccat gaagttctgg aagtccgtcg ggtcgccgat cttgatccga gcgatcatct
    12781 ggagcatccg gttctcgacc cgtggccaga tcgagcgcgg gatgaacgcg cgcgatgcgg
    12841 cggagcactt ctgcccctgg tattcgaacg cgccgcgaac gagcgccgtc gcgagcgacg
    12901 ggacgtcggc ggaccggtgc gcgaagacga aatccttgcc gccggtctcc ccgacgatgc
    12961 gcgggtagta gtcgtagctg cggatgttgc ggccgatcgc ctcccacatc gccttgaaca
    13021 cgtcggtcga gccggtgaag tgaataccgg ccagctcgcg gctggccaat accgggtcgc
    13081 cgacttccga ccccgatccc gggaccatgt tgatcacgcc cggcggtagg ccggcccgtt
    13141 cgaggatctc catgatgaaa tgcgccgagt agacggcgga ggaggccggc ttccacagca
    13201 ccgtgttgcc catcatcgcc ggggccgtcg gtaggttgcc cgcgatgctg gtgaagttga
    13261 acggcgtcac ggcgaagacg aacccctcga gcggccggtg ctccagatag ttccacaccc
    13321 ccggtgccga ttccggctgt tcggagtaga tgcgctccat gaactcgacg ttgtagcgcc
    13381 agaagtcgat cagctcggcg gccgagtcga tctcggcttg gtgcactgtc ttcgattgcc
    13441 cgagcatcgt cgcggcgttg agcaccggac gatacggacc ggcaagcaga tcggccgcct
    13501 tgaggaagat cgaggcacga tggtgccacg acatcttcga ccatgttcgg tgcgcctcgc
    13561 gcgccgcctt gatcgcccgc tcgacctcgc gcttcccgca cttgtgccac gtggccaaga
    13621 cgtgcgcgtg gtcgtgcggc atcaccgctt tgccgatctt gccggtacgc acccggcggc
    13681 cgccgatccg cgccgggatc tccgtctcac gcccggcgag ctcgtcgagc cactgctcga
    13741 tctctcggcg ttcggggctg ccgggggcgt ggtcgcgtac cggttcgttg gtcggcacgg
    13801 ggacggtgaa aatgccgttg gacatcagcg gtctcctcgc ggctgccggt tggcgagatc
    13861 gcgcattcta gcagccgctc tcgccgcgcg gccaagcgac gaagcggagc gccgagatct
    13921 taatggttcg tcgcgtcgag cgcgtcctgc acttcggcgg cgtgccgatc cgtgtgctgg
    13981 cgcgcttcgt cgagtccggg cagcccgtcg tccggcgcgc cgcccgcatc ggcgtcaccg
    14041 tgtgcggcgc gcgggtcggc gccggtccgc ggccgggaca gctcgatggc gctgggtgcc
    14101 agctcgcgcc acgagcgcgc gacgagcagc atcaccacga cgagggcgat gagcagcacg
    14161 accatcccga ccgccgatcc gcgtctcatc tcgcgaattt agtcccgcgg ccggccgccg
    14221 gcaacgatcg gggcgcgccg gcgccacttg ccccgccggc gcggatcctt cacattggat
    14281 ccgatgtcgt gctccggtcg cctcgtcgtg ctggccgtgc tcgccgcgat cgcgggcggc
    14341 ggggcggtca ccggcgcgcc ggacgatgac gtcgccgccg cggtcgtcga tcgcctgcag
    14401 atcgagcggc gcgagcgcgg cgcgccgccg ctcgagcggc gcccggggct cgaccgggtc
    14461 gcgctcgagc gggcgcgcga gatcgccgcg ctaccccaca gcgggcgtct gtcccacgcg
    14521 cagccggtcg gcgatcagct gcgcgcggcc ggcatccgct ggttcagccg cgccgcggcg
    14581 cacgtcgaca tggtgcgtgg ctacctgcgg ccggagaccg gtttcctgcg gagctggcgg
    14641 gatcacgaca cggcgtggac caacgcgctg gcgtcggatt tcaccacggt cggtgcggcg
    14701 accgcccgca gcgcggacgg ttggatcgtc ttggtgatcg tgctggtcgt cgacctaccc
    14761 tatcccgagg acaccggccg gctggagcag gccgtcatcg agctcgtcaa tcgcgttcgc
    14821 gacgagcacg gtctcggccg gctcgccggt cgcgccgatc tcgcggtcgt cgcccgcagg
    14881 cacagcgagg agatggcacg ccgcgatttc gtcgcccatg tcgacccgga tggccacggc
    14941 accgccgacc gcgtcctacg ggcgggtctc cgcttcctcc agctcggcga gaacatccac
    15001 atgaaccgcg gagcgcgcga tccggccgcg ttcgcggtcg aacgatggtt gggcagcccg
    15061 ggacatcgcg agacgatgct cgatccggcc ttcgtcgaga cgggggtcgg cgcggcgatc
    15121 gccgacgacg ggcgcatctt catcacccag gtgttcatcg ccccggcgcc gacgcgctga
    15181 cgtccggcca cgacgcatga gccgaccgcg ggccaggtac gcccccagtc cgacgggcga
    15241 cctgcatctc ggcaacgcat cgagcgcgct gctcgcctgg ctctccgttc gtgccgccgc
    15301 cgggaggttc gtcgtgcgcg tcgaagatct cgaccgggcc aggtcgaggc aagagctcgt
    15361 cccgcgcatt ctcgacgatc tggcctggct cgggctcgac tgggacgagg gaccggatcg
    15421 cggtgggccg cacgcgccct acgagcagtg gcggcgtctc gagcgctacg aggccgcgct
    15481 ggccgagttg caacgggccg accgcgtcta tccgtgcttc tgcagtcgcc gcgacgtggc
    15541 cgcggctgcc agcgcaccgc agcttcccgg tgacgaagtc cgctacccgg gcacctgccg
    15601 cgaactggac tccgcccggg cgcggcgccg gatcgcggcc ggcgagccgc acgcgcttcg
    15661 tttccgcgtt ccccgcggag cgcgggtcga gttcgacgac atcgtgcacg gtcgggtcgt
    15721 cgatccggcg gggacgccga ccggcgactt cgtggttcgt cgcgccgacg gcgttcccgc
    15781 ctaccagctt gccgtcgttg tcgacgacgc cgcgatgaag atcaccgagg tcgtgcgcgg
    15841 cgacgacctg ctgccgtcga ccgcccgcca gctgctgctg ttcgaggcgc tcggagtcgc
    15901 accgccgcgg ttcggacacg tgccgctgct gctcggcccg gacggcgttc gcttggcgaa
    15961 gcggcatcga ggctcgtcgg tgggcgagct gcgggagcgg gggctcgacg ccgggaaact
    16021 cgtcgggcga ctggccgagc ggttggggct gaccgcaaac ggcgacccgg tccggccgtc
    16081 cgagctcgtc tccggcttct cgctcgcgcg gctgcgcgcg gcgccaggcg gcatcctcat
    16141 cgatccgacc ggctggtcgt gagcctcgga gctagtcgcg ctcgtcctgc ggaattcgcg
    16201 gcagcggttc atcgcgctcg gccagcatgt aagccatcac ggccatcgcg gcgacgttga
    16261 ggtcgagctt gtctcgcgcg accttgtcca gcgtatcggc ttcggtgtgg tggtagtcga
    16321 agtagcgcgt cgggtcggga agctggccga tctgcggcac gccgaacttc ttcatcaacg
    16381 agatatccgc gccgccaccg cgggggatga cccggtcggc gccgatcgag accaattgcg
    16441 cggcgatttc gccgacgatg tcggcaccac ccgcgccggc cgtcaccccg aagccgatcg
    16501 gggcgtcgcc gccgaggtcc gattcgatcg cggcgacgtg attcgtcatc tgatcgacgt
    16561 agcgttcggc gtagtcccgc gcaccgcgca acccgttctc ctcgttggtg aacagcaccg
    16621 cacggatcgt cctcttcggg atcagatcgt gctgtttcag cagtcgcatc gtttccatca
    16681 cgatggcgca gccggcgccg tcgtcgtgtg cccccgtccc gacgtcccac gagtcgaggt
    16741 gcgcgccgat gacgacgatc tgctcgggcc gctcgcggcc gggcagctcg gccagcacgt
    16801 tggccgagac ggcgtcgggc agcatccgtg cgccgagccg cagccgcacg cgaaccgtct
    16861 cgccctggcg cgtcaacctc gaaatgagca tcgcgtcctc gccggatacc gccgccgccg
    16921 ggatcggcgc cacgtcgtcc tcgtagcgca gcgcgccggt gtgcggcagg cggtagtccg
    16981 ccgtgccgag cgagcggatc agcatcgcga tcgcaccctg cttggccgca gccgacgcgc
    17041 cgcgcgagcg gagtttcgac agcgcgtcgt agccgttgtc ggtatcgaag ccgcgttcca
    17101 tcgcgcgatc gaacaggacg atcttcccac gcgcgccttc gccgagcgtt tccagcgcct
    17161 cgaagccgtc gacgacgacg acctctcccg tgacgccgtc gtccggcgtg ccgatggtgc
    17221 cgcccaagcc gaggatcgcg aggtcgtgcc gcgcggggac gacgatctgc gcggattcct
    17281 cgccacgcac ccagtgcggg accgccacca cgtcggtcca gacgcgcggc agtccgtccc
    17341 gctcgaattc cgccgcggcc cactcgaccg cccggtcgag ggccgctgag ccgctcaatc
    17401 gatgaccgat ccgatcggtc agccaggcga gcttgtcgta cgcccggctt tcctccttcg
    17461 cgatctcaac gatccgcgcg gcgatcggat cgtcggccgc ccggatcgag cccgcgtacg
    17521 agcatgcgca cagggcgagt agcgtccatc gcactggtgg cgtgtgcatg attcgcgcat
    17581 tatagacgcg acccttcgcc gagcgggtga ggtgcgagcc ggatgatcgt caagccgttc
    17641 cgcgcgtgga gaccgccggc cgagctggct gcggtcgtgg ccagcccccc gtacgacgtg
    17701 ctcagctcgg ccgaggcgcg ccggttggcc gcgggcgagc cgcgttcgtt cctgcacgtg
    17761 accaagcccg agatcgacct cgaccccgcc accgacccgt acgcggacgc ggtctacgcg
    17821 accggagcgc gcaactttcg cgcgatgatc gagcgcggct ggttggtgcg cgacgagcgt
    17881 gcgtcgtact acgtctaccg tctgacgatg gacggccgcg cgcagaccgg actgctcggc
    17941 gcggcggggg tcgacgacta tcgaacgggt cggatcaagc gccacgagca cacgcggccc
    18001 gagaaggaaa aagaccgggt tcgcctgaac gatgcgatcg gcgcgcatcc cgggccggtt
    18061 ttcctcgcct accgtggact tccggagctc aacgcgatcg tgaacggcgt ggtggcggac
    18121 ggaccgcgga ccgatttcgc ggcggacgac ggcgtacggc acgaggtgtg ggtcgtcgac
    18181 gaaccggcga cgtgccgcaa gatcgagacg ttgttccgcc acgttccggc gacctacgtc
    18241 gccgacgggc accatcgcgc cgcggccgcc gtcaaggtcg ccgagctgcg tgactcgcgc
    18301 cagccgccgg gagcgtcggc cgatcgagaa tcggcatggc ggttcttcat ggcggcccac
    18361 ttcccgtacc gccagctgcg ggttctcgac tacaaccgcg tcgtccgcga tctgaacggg
    18421 atggagccgg cgacttttct cgatcgcctc gcccggcgtg gctttcacgt cggggccgac
    18481 catcgcaagc gacgcccgcc gtcgcccgag accttcggca tgtatctcga ccgccgctgg
    18541 tacctgctga ccccgcgtcc cgagctgata ccgaccgacg tcgtcggcaa gctcgacgtg
    18601 gcgatcctca acgaccaggt gctgtcaccg ctgctcggca tcggtgaccc gcgcaccgat
    18661 cggcgagtcg agttcgtcgg tggtatccgc gggatggacg agctcgagcg cctggtcgac
    18721 tccggcgatt acgaggtcgc gttcgcggtc catccgacga gtctcgagca cgtaatggcg
    18781 gtcgccgatg ccgggcgcgt gttgccgccg aagagcacgt ggttcgagcc gaagctgcgt
    18841 tcggggctga tcgtccaatc gttcgacgaa gggctgtagc gggctggctc agccaccacg
    18901 atgcttgcgc gacagcttcg ccatcagctt ctgcttgacg ccgggctgca gacccggccg
    18961 gcgaatcagc ttgtcgagcg tgatctcgtt catctgggcg acgatcttgt ccgcggtcgg
    19021 gaaggtgacc cgcggatggg tcgcgaccgc gatccggacc tgttcgtcgt gattgaatcg
    19081 gggattggat gcgatcgact cgagcgtcgc gggcaacaag tcggttcgcc tgagcagcga
    19141 gtggatttcg ggcagattgg tcgtcgggt
//
LOCUS       DASPGN010000584         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_345745, whole genome shotgun sequence.
ACCESSION   DASPGN010000584 DASPGN010000000
VERSION     DASPGN010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345745"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /gene="aroF"
                     /locus_tag="VD788_16815"
     CDS             <1..781
                     /gene="aroF"
                     /locus_tag="VD788_16815"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXV77974.1"
                     /translation="VGGVEFGGVEPVVVAGPCAVESYDQTLAVARAVRAAGVEVLRGG
                     AFKPRTNPHSFQGLGLEGLEILSAVRRETGLAVVTEVLDPRLVETVSRHADMLQIGSR
                     SMHNYPLLSEVGKGSLPVLLKRGFSATLEEWLCAAEYVAKEGNRSIVLCERGLRTSCH
                     DTYARSVLDLNVIGPLRRATPLPVIVDPSHATGEWTLVEPVARAAIAAGAQGLLIEVV
                     ADGVDRASLRCDARQGVPPAVLRSIVRHGRETASRHGEVLG"
     gene            950..1384
                     /locus_tag="VD788_16820"
     CDS             950..1384
                     /locus_tag="VD788_16820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV77975.1"
                     /translation="MGGETTTTAVELVLPSDVKLVDLVHATSEKMAEFAGFDADEALN
                     VGLALREAVINAMVHGNGQDPRLKVHISLVANATQLEAKIRDEGQGFDPDSEPDPTAE
                     ENLLNTSGRGLLLMRAFVDEVRFTQHEKRGMEITLTKKRTAS"
     gene            1424..1759
                     /locus_tag="VD788_16825"
     CDS             1424..1759
                     /locus_tag="VD788_16825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXV77976.1"
                     /translation="MKATVRQIGKVSVVDLSGKITIGEGDVILRERVTELLDGGNQNI
                     LLNLEKVGYMDSAGIGELVACYKRAKEKDGTVKLLNPSGKVYDLLQLTKLEEVFETYK
                     DEKEALVSF"
     gene            complement(1763..>2010)
                     /locus_tag="VD788_16830"
     CDS             complement(1763..>2010)
                     /locus_tag="VD788_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77977.1"
                     /translation="ERDEYGGDGLPRRARRSDGEGRPAPLQPSLFGWSATAVSNPSQQ
                     AAAAEVLAEIAARDPDQLTPIDALALLARWRQRLARD"
BASE COUNT          329 a          646 c          731 g          304 t
ORIGIN      
        1 tgtcggcggc gtcgagttcg gcggtgtcga gccggtggtc gtcgcggggc cgtgcgccgt
       61 cgaatcctac gatcaaacgc tggccgtggc acgcgcggtc cgcgcggcgg gcgtcgaggt
      121 gctgcgcggc ggcgcgttca agccgcgtac caacccccat tcgttccagg ggctcggcct
      181 cgaggggctc gagatcctgt cggcggtgcg acgcgagacc gggctcgccg tcgtgaccga
      241 ggtgctcgac ccgcggctgg tcgagacggt ctcccgccac gccgacatgc tgcagatcgg
      301 ctcgcgctcg atgcacaact acccgctcct ctccgaggtg ggcaagggtt cgctgcccgt
      361 gctgttgaag cgtggcttca gcgcgacgct cgaagagtgg ctgtgcgccg ccgagtacgt
      421 cgccaaggag gggaaccgct cgatcgtgct gtgcgagcgc gggctccgca cctcctgtca
      481 cgacacctac gcgaggagcg tgctcgacct caacgtgatc ggaccgcttc gtcgtgcaac
      541 cccgctgccg gtgatcgtcg atccgtcgca cgcgacgggc gagtggacgc tggtcgagcc
      601 ggtcgcgcgg gccgcgatcg ccgccggggc ccagggcctg ttgatcgagg tggtggccga
      661 cggcgtcgat cgggcgagcc tgcgctgcga cgcgcggcag ggagtaccgc cggcggtgct
      721 gcgatcgatc gttcgccacg gccgcgagac cgcttcgcgg cacggcgagg tgctgggttg
      781 acggggggaa cggctcggcc agcgccgggc ccgacgttga cttggcaggg tcccacggct
      841 attcttcttg ccgtttgtaa ccgtccagtc gcggggagac tcgaccggca ggcgcccggc
      901 ggtccgccgg attcccggca gcggggtggt gtgccggagc aaggagcgca tgggcggaga
      961 gacgacaacg accgccgtcg agctcgtgct gccgagcgac gtcaagctcg tcgatctcgt
     1021 ccacgcgacg agcgagaaga tggcggagtt cgccgggttc gacgccgacg aggcgctcaa
     1081 cgtcggtctc gcgttgcgcg aggcggtgat caacgcgatg gtccacggta acgggcagga
     1141 tccgcggctc aaggtccaca tttccctcgt cgccaacgcg acgcagctcg aggccaagat
     1201 tcgcgacgag ggccaggggt tcgaccccga cagcgagccc gatccgaccg ctgaggagaa
     1261 cctgctcaac acgtcggggc gcgggctact gctgatgcgg gccttcgtcg acgaggtgcg
     1321 cttcacccag cacgagaagc gagggatgga aatcacgctg accaagaaac gaacggcttc
     1381 ctgatcggag gcttggcaca gacgcgcacg aaggagtgga gcaatgaaag cgacggttcg
     1441 gcagatcgga aaggtctcgg tcgtggacct ttccgggaag atcacgatcg gcgagggcga
     1501 cgtgatcctc cgcgagaggg ttaccgagct tctcgacgga ggcaatcaga acatcctgct
     1561 caatctagag aaggtcgggt acatggactc ggccgggatc ggcgaactcg tcgcgtgcta
     1621 taagcgcgcg aaggagaagg acggaacggt caagctgctc aacccgtcgg gcaaggtcta
     1681 cgacctgttg cagctgacca agctcgaaga agtgttcgag acctacaagg acgagaagga
     1741 agcgctcgtc tcgttctgag ggtcagtccc gcgcgagccg ttgccgccag cgcgcgagca
     1801 acgccagcgc gtcgatcggc gtcaactggt cggggtcgcg cgcggcgatc tcggccagca
     1861 cctccgccgc ggcagcctgc tgcgacgggt tcgagaccgc cgtggcgctc caaccgaaca
     1921 ggctcggctg caacggggcc ggccgtccct cgccgtcgct gcggcgcgcg cgccggggta
     1981 agccgtcccc gccgtactcg tcgcgctcga
//
LOCUS       DASPGN010000585         5167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1281072, whole genome shotgun sequence.
ACCESSION   DASPGN010000585 DASPGN010000000
VERSION     DASPGN010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281072"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..685)
                     /locus_tag="VD788_16835"
     CDS             complement(<1..685)
                     /locus_tag="VD788_16835"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HXV77978.1"
                     /translation="MLVLEDGRTFRGRSFGGVGERTGEVVFNTAMTGYQEVLTDPSYA
                     GQIVTMTCPEIGNYGTNGTDVESTRPHLEGFVVRQVSELPSNWRATQGLPQYLREHGI
                     PGIAEIDTRALTRHLRSRGSMKGVISSVDRDPASLARKARQAPGLDQRDLVAAVSCAA
                     PYGWDRPREAQWSAAASPGGGGARPHCVAFDFGIKQNILRMLHEHGFRVTVVPAGTTA
                     REVTALHPDG"
     gene            complement(716..1546)
                     /locus_tag="VD788_16840"
     CDS             complement(716..1546)
                     /locus_tag="VD788_16840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXV77979.1"
                     /translation="MQLTVLRAGELWLDGGAMFGIVPKTLWGKERMADDLNRIRLSMN
                     VLLIDDGRQKWLVDTGAGAEWDEKTRRIYRLDPRPPDDYLADAGATPEQIDVVISSHL
                     HFDHAGGHTRRAGQGETVASFPRASYVVQRAELEIAREVNERTRASYRAADFEPLAAE
                     PGRLRIVDGETRLSRQVSVELAPGHTPGMQIVRVEAGGETVCFLADLVPTTSHVRYPY
                     IMGYDLEPLRTLATKKRVLRRALDEGWTLVFEHDAERPVARLAEAGGKLTTMACGPVH
                     "
     gene            1887..1961
                     /locus_tag="VD788_16845"
     tRNA            1887..1961
                     /locus_tag="VD788_16845"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1919..1921,aa:Val,seq:gac)
     gene            2015..3985
                     /gene="thrS"
                     /locus_tag="VD788_16850"
     CDS             2015..3985
                     /gene="thrS"
                     /locus_tag="VD788_16850"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXV77980.1"
                     /translation="MEAPERSVRITLPDETVREVPHGSTPAELAASLGRRLAKDAIVA
                     RVDGELVDLSRPIRRDSTVELLTRDTKEALDVLRHSTAHATAQAVQELFPGTKIGQGP
                     VIEDGFYYDFDREIPFSDQDLAAIEARMAEIVSRDLGIERIEVPREEAIRHFEAEHEP
                     YKLYFARTKGGETVSIYRQGDWDDFCRGPHVPSTGRLGAFKLLSVAGAYWLGNERNKM
                     LQRIYGTAFFTRKELDEHLELLEQARRRDHRKLGRELGLFSFHAEAPASPFFLPRGAT
                     LYNSLVEYVRGLYRQYGYDEVITPQVFDASLWHRSGHYEHYRNNMYFTEVDEREYAVK
                     PMNCPSHCLIYSEGRHSYRDLPLRLADFGRLHRYELSGATAGLTRVRTFSQDDAHIFC
                     TPEQIPVEVASVTRMILDCYELFGFEVKIVLSTRPEDRAGDDALWDRAEAALHGALTA
                     QGHRFTTGEGEGAFYGPKIDFVVRDALKREHQLGTIQLDYVAPERFDLRFIDPQDREQ
                     RPVMIHRAMLGSLERFIGILIEHTGGAFPFWLAPEQLRVLSITERVVGYATEIAGKLL
                     AGGLRATTDLRNEKIGAKIRAAQLERVPVMLVVGDREAADGTVAVRLRRAGDQGTIDA
                     ETFAGLAVAWQRDRAAEPSERPNDVRGSRARD"
     gene            3995..4198
                     /gene="rpmI"
                     /locus_tag="VD788_16855"
     CDS             3995..4198
                     /gene="rpmI"
                     /locus_tag="VD788_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXV77981.1"
                     /translation="MPKLKSHRGAAKRFKSTASGRFKRGKAYKSHILTKKSTKRKRKL
                     DTPTLVSDADQRRVERMLPYGSK"
     gene            4294..4656
                     /gene="rplT"
                     /locus_tag="VD788_16860"
     CDS             4294..4656
                     /gene="rplT"
                     /locus_tag="VD788_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXV77982.1"
                     /translation="MPRVKRGNKRRLKRKRILRRAKGYYLAKSKLYRIAREQLMRSER
                     YAYRDRRQKKRQFRRLWIVRINAAARLHDISYSTLMHGLKTAGVRLDRKILAEIAVHD
                     PAAFGQIARTARQAASSV"
     gene            4668..>5167
                     /gene="pheS"
                     /locus_tag="VD788_16865"
     CDS             4668..>5167
                     /gene="pheS"
                     /locus_tag="VD788_16865"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXV77983.1"
                     /translation="MQQELERIEREFEARLSAAGDDLREIDSVRVGFLGKKGQVTALM
                     KRLASLPADDKPAAGKAINELKRRVSDGIDRALAAARLGRRERELGAVDVDVTLPPRQ
                     PWVGSLHPVTRTRRDLERIFVEMGFRIETGPEVEDDFHNFQALNIPEDHPARDDTDTF
                     YLEDGNL"
BASE COUNT          852 a         1784 c         1736 g          795 t
ORIGIN      
        1 cgccgtccgg gtggagcgcc gtcacctccc gggcggtggt gccggccggt accaccgtca
       61 cgcggaagcc gtgctcgtgg agcatccgca ggatgttctg tttgatgccg aaatcgaagg
      121 cgacgcaatg cgggcgcgcc ccccccccgc cggggctggc tgccgcgctc cactgcgcct
      181 cgcgcggccg gtcccagccg tacggcgcgg cacagctgac ggcggcgacg agatcgcgct
      241 ggtcgagacc cggtgcttgc ctggctttgc gtgcgagcga cgccggatcg cgatcgacgg
      301 acgagatcac tcccttcatc gaaccgcggc tgcgcaggtg gcgcgtcaac gcgcgggtgt
      361 cgatctcggc gatcccgggt atgccgtgct cgcgcaggta ttgcggcagt ccttgcgtcg
      421 cgcgccagtt ggacggcagc tcggagactt ggcgtacgac gaagccctcg aggtgcggcc
      481 gcgtcgattc gacgtcggtg ccgttggtgc cgtagttgcc gatctcgggg caggtcatcg
      541 tcacgatctg cccggcgtag gatggatcgg tcaggacttc ttggtagccg gtcatcgcgg
      601 tgttgaacac gacctcgccg gtgcgttcgc cgacgccgcc gaaagagcgt ccccggaagg
      661 tgcggccgtc ctccagtacg agcatcgctt ccacgactcg cccccgtccc cgcggtcagt
      721 gaaccggccc gcaggccatc gtcgtcagct tgccgccggc ctcggccaga cgggccaccg
      781 gacgctcggc atcgtgttcg aagaccagcg tccagccctc gtcgagcgcc cgacgcaaga
      841 cgcgcttctt ggtggccaac gtgcggagcg gctcgaggtc gtagcccatg atgtacgggt
      901 agcgcacgtg gctcgtcgtc ggtaccaggt cggcgaggaa gcagacggtc tccccaccgg
      961 cctcgacacg gacgatctgc atacccggcg tatgtcccgg cgcgagctcg accgagactt
     1021 gtcggctgag tcgcgtctct ccgtcgacga tcctcagccg ccccggctct gcggccagcg
     1081 gctcgaaatc ggcggcccgg taggacgcgc gggtccgttc gttgacctcg cgcgcgatct
     1141 cgagctcggc tcgctgcacg acgtaactcg cccggggaaa cgacgcgacc gtctctccct
     1201 gcccggcccg ccgggtgtgg ccgcctgcgt ggtcgaagtg caggtgggag gagatcacga
     1261 cgtcgatctg ctccggcgtg gcgcccgcgt cggcgagata gtcatccggc ggacgcggat
     1321 cgagccggta gatccgccgg gtcttctcgt cccattccgc cccggcgccc gtgtcgacga
     1381 gccacttctg ccgcccatcg tcgatcaaca gcacgttcat cgacagccgg atccggttca
     1441 gatcgtcggc catccgctcc ttgccccaca gggttttcgg cacgatgccg aacatcgcgc
     1501 caccgtcgag ccagagctcc ccggcgcgaa gtaccgtgag ctgcacgtcc cctttctcga
     1561 atcggatcaa tcgtcggtcc cctgcagccg aggccgcgta ggagccaccg ctcgacgcgg
     1621 gatcgacccc ccctgccgat gtccgatccg ggcgaccgtc tcgccgtact ttcgacacct
     1681 cgggctcgac caccccacgt cgcgctcgcc gcgagccggc gtgcgatggt atcgaatgcg
     1741 aaacggggaa tcaacctcgc gctcgcgaca agagccggcg cgggcgcgtg gaactccttt
     1801 cgttgacgtt gtttggggcc tgtggtacct tcgcccgtca tcggggtccg gcccggccgc
     1861 gctcgtgtgg ccggaggtgt ccgaacaggc gcgtagctca gcgggagagc gctaccttga
     1921 cacggtagag gtcggcggtt cgaaaccgcc cgcgcctacc acccgccggt ccgcgaaccg
     1981 gttccctggt gccgaaaggc gcggatcgtc aacgatggaa gctcccgagc ggtcagtacg
     2041 aatcacgttg ccggacgaaa cggtgcgcga ggtcccccac ggctcgacac cggcggagct
     2101 cgccgccagc ctgggccggc gcctggcgaa ggacgcgatc gtcgcccgcg tcgacggcga
     2161 gctggtcgac ctgtcgcggc cgatccgccg ggactcgacg gtcgagctcc tgacccgcga
     2221 cacgaaagag gcgctcgacg tgctgcgtca ttccaccgcg cacgcgaccg cccaagcggt
     2281 gcaggagctc ttccccggta ccaagatcgg tcagggtccg gtgatcgaag acggcttcta
     2341 ctacgacttc gatcgggaga taccgttcag cgaccaggat ctcgccgcga tcgaggcccg
     2401 catggccgag atcgtctcgc gcgacctcgg gatcgaacgg atcgaggtgc cgcgggagga
     2461 agcgatccgg cacttcgagg ccgagcacga gccgtacaag ctctatttcg cccgcaccaa
     2521 gggcggcgag acggtctcga tctaccgtca aggcgactgg gacgactttt gccgcggccc
     2581 acacgtcccc tcgaccgggc ggctcggcgc gttcaagctc ctgtccgtcg ccggtgccta
     2641 ctggctcggc aacgaacgca acaagatgct gcagcgaatc tacggaacgg ccttcttcac
     2701 ccgcaaggag ctcgacgagc acctcgagct gctcgagcag gcacgacgac gcgaccaccg
     2761 caaactgggg cgcgagctcg gcttgttctc gttccacgcc gaggcgccgg ccagcccgtt
     2821 cttcctgcct cgtggcgcga cgctttacaa ctcgctcgtc gagtacgtcc ggggtctcta
     2881 tcggcagtac ggctacgacg aggtgatcac accccaagtc ttcgacgcgt cgctctggca
     2941 tcgctccggt cactacgagc actaccgcaa caacatgtat ttcaccgagg tcgacgagcg
     3001 ggagtacgcg gtcaagccga tgaactgccc gtcgcactgt ctgatctact ccgaaggccg
     3061 tcactcctac cgggacctgc cgcttcgcct cgccgatttc ggtcgcctcc accggtacga
     3121 gctttccggc gcgacggccg gcctgactcg cgtgcgcacc ttctcgcagg acgacgccca
     3181 catcttctgc accccggagc agatccccgt cgaggtggct tcggtgacgc ggatgatcct
     3241 cgactgttac gagctgttcg gcttcgaggt gaagatcgtg ctttcgactc gcccggagga
     3301 ccgcgcgggt gacgacgcgt tgtgggaccg cgccgaggcc gcgctgcacg gtgcgctgac
     3361 cgcgcagggt caccgcttca cgaccggcga gggagagggc gcgttctacg gacccaagat
     3421 cgacttcgtc gtccgggatg ctctcaagcg ggagcaccag ctcgggacga tccagctcga
     3481 ctacgtggcg cccgagcgct tcgacctgcg gttcatcgat ccacaggatc gagagcagcg
     3541 cccggtgatg atccaccgcg cgatgctcgg ctcgctcgag cgcttcatcg gcatcctgat
     3601 cgagcacacc gggggggctt tccccttctg gctggcgccg gagcagctcc gcgtgctgtc
     3661 gatcaccgag cgagtggtgg gctacgcgac ggaaatcgcc gggaaactgc tcgccggcgg
     3721 gttgcgcgcg acgaccgact tgcgcaacga gaagatcgga gccaaaatcc gcgcggccca
     3781 attggagcgg gtgcctgtga tgctggtggt cggcgaccgc gaggcggccg acggaacggt
     3841 tgccgtccgt ctgcggcgcg cgggggacca gggaacgatc gacgccgaga cctttgccgg
     3901 cctcgccgtg gcttggcagc gggaccgggc cgccgagccg agcgagcgac cgaacgacgt
     3961 ccgagggtcg cgtgcccgcg actgaggaga cacgatgccg aagctgaaat cccatcgcgg
     4021 cgcggccaag cgcttcaagt cgaccgccag cggtcgattc aagcgcggca aggcgtacaa
     4081 gagccacatc ctgaccaaga agtcgaccaa gcggaagcgg aagctggaca ccccgaccct
     4141 ggtctcggac gcggaccagc ggcgagtcga gcggatgctg ccctacggca gcaagtagat
     4201 cgcggccgac gcccgagcga accaggcgac aagtggacgc ggcaggcgtc ccgccgagcg
     4261 ggtcgaacgg ggcccgacgg caaggaggat tcgatgcccc gagtcaaacg cggaaacaag
     4321 cggcgcctga agcggaagag gatcttgcgg cgcgccaagg gttactacct ggcgaagtcg
     4381 aagctctacc gcatcgcgcg cgagcagctg atgcgctcgg agcggtacgc ctaccgcgac
     4441 cgccgccaga agaagcgcca gttccgccgg ctgtggatcg tccggatcaa tgccgcggcc
     4501 aggctccacg acatttccta ctcgacgctg atgcacggcc tgaagaccgc cggagtccgc
     4561 ctcgaccgca agatcctggc ggagatcgcg gtccacgatc cggcggcgtt cgggcagatc
     4621 gcgcgaacgg cccgacaggc ggcctccagc gtctgaccga ggacgcggtg caacaggagc
     4681 tcgagcgcat cgagcgcgag ttcgaggctc gcctcagcgc ggcgggggac gatcttcgcg
     4741 agatcgattc cgtccgcgtg ggttttctcg gcaagaaggg gcaggtgacg gcgttgatga
     4801 agcggctcgc gtcgcttccg gccgacgaca agcccgccgc cggcaaggcg atcaacgagt
     4861 tgaaacgccg cgtcagcgac gggatcgacc gcgcgctcgc cgccgcccgg ctagggcggc
     4921 gggagcgcga gctgggcgcg gtcgacgtcg acgtgacgct ccccccacgc caaccgtggg
     4981 tcggatcgct gcacccggtg acccgcaccc gacgggacct cgagcggatt ttcgtcgaga
     5041 tgggcttcag gatcgagacc gggcccgagg tcgaagacga cttccacaac ttccaggcgc
     5101 tcaacatccc cgaggatcac ccggcgcgcg atgacaccga cacgttctac ctcgaggacg
     5161 ggaacct
//
LOCUS       DASPGN010000586        14066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_930181, whole genome shotgun sequence.
ACCESSION   DASPGN010000586 DASPGN010000000
VERSION     DASPGN010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 14066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14066
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930181"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /gene="rsmH"
                     /locus_tag="VD788_16870"
     CDS             complement(<1..806)
                     /gene="rsmH"
                     /locus_tag="VD788_16870"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXV77984.1"
                     /translation="MIAVASGEETVMTPESVHEPVLLRETIDWLAVRAGSTVVDATLG
                     PGGHAHALLERIGPSGRLIGIDRDPQALVVATGRLARFGPAFTAVRGNHTDLLRLLRA
                     AAVERVDAVLLDLGLSSLQLASPERGFSFREDGPLDMRMDPQSAPTAAELLAGAGEDE
                     LRRLLWRYGEERRARAIARAIVAERVRRPLETTRQLAELVARVLGPAARRYRIHPATR
                     TFQALRIAVNREVDDLERLVADATSVLRCGGRLAVISYHSLEDRAVKHSLR"
     gene            complement(823..1263)
                     /locus_tag="VD788_16875"
     CDS             complement(823..1263)
                     /locus_tag="VD788_16875"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77985.1"
                     /translation="MLRGNALAKVDEKGRLKLPASFRAVIEPEYGNEFFVTSLRGDSV
                     RLYPMQVYSSLEQRLMGASTLKPAVMKLRTSLNFFGQAAAMDPQGRILIHPLLRERAA
                     IDGEVAVLGQQNFLEVWNRAQFEERLERDPLTDADLAELASMGF"
     gene            1557..2726
                     /locus_tag="VD788_16880"
     CDS             1557..2726
                     /locus_tag="VD788_16880"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXV77986.1"
                     /translation="MNDLRTICARSARHAPAVGRPLAPVIVPSSTFGFDTLADVDRYY
                     DAGDGYLYSRYGNPSVERVENLLAELEGAEGAVCFSSGMGAITTALSAVTRAGDRVVA
                     QRELYGGTSEYLRGVLPLGGVKVDWITTDELARLEPARLVGARALYLESPTNPTLRIV
                     DLERVATIAREVAVVTLVDATFATPALERPIERGIDLVVHSATKYLGGHGDLIGGVVA
                     GRGESIGAIRERRRITGATMDPFSAFLLHRGLRTLAVRVEAHCRGAARVAAHLERAAG
                     VERVIYPGLASHPDHELARRRMPGYGGMVSFVISGGAPAAERVHDALTLFAKAGSLGS
                     IESLVSIPARMSHRHLGAEARLEAGIPDGLLRLSVGLESPEDLIADLDRALAAAG"
     gene            complement(2803..3489)
                     /locus_tag="VD788_16885"
     CDS             complement(2803..3489)
                     /locus_tag="VD788_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77987.1"
                     /translation="MHKKLTIFAAVSTIVALGTVALADGPRGLPPRYAPRSMDGRIHQ
                     VEVLASQTTIATWAYRNGARFDIAVSTAQADGDWSEPVFFGRDDERDQIEPVVAADAH
                     GNVFLAYTDRDEGRVMLAIRPAGRTDWSTPLPVTPLASGAETPSLLVTEDRLVVAFQI
                     DRQIHLFQLPLPGDGTTSTLGLTDGSDPVGHEDGSPPPSDENQGIDSGASGTSRYFAP
                     FDKKIYPPTR"
     gene            complement(3482..4675)
                     /locus_tag="VD788_16890"
     CDS             complement(3482..4675)
                     /locus_tag="VD788_16890"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXV77988.1"
                     /translation="MASQPQAAALKGSVKLGSYLRKLRTGYGYSLRRVEERARAEGGE
                     IDNSQLSRYEKGVCYPSFDKLRILANVFNVSIQSFSDVVDLESFEALKPDTNDAAELQ
                     ERGLDALKAGDTGLSFALFERAIELLSDGPQDSETQEQLGRARINQAAVLNRLGKLAL
                     AEQELRSALRLSERLSPVLQARALLALALIHTDQGDHFLSEMEADRAYQIAANASLPL
                     EAAKALNLFGHVLHQRGAPTEAIERFREAGKLFARCGNDFEANRVRINTGTCYVALGK
                     YREGIRLLREALNEARNAGHRRLEAHAWSNLGEAYYRQDDLRRARGCFLESDALAHHN
                     GEKQPDILFFNAFYEWKMAIQTENPTREKIAFGRLKALRSSLERRTVEVDAFDDYVER
                     GKPHA"
     gene            5003..6328
                     /locus_tag="VD788_16895"
     CDS             5003..6328
                     /locus_tag="VD788_16895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV77989.1"
                     /translation="MLFGFLLAVLVAITLDLAFRQLSVSVLERRLDLGMVEARDIARI
                     VSDVGRDDSGIDFSLVRERGDYLQDVIHRRISMRVFVHHVEVVDRFGIQQLFVANTAF
                     QHEPPRVVPDLSFPEDWPKSFEQIVQWPLTRSEGMVKLGVSSQPILDELSMLRSSLRK
                     KLMLGAALALALLVAGFFYVLYLLRKNRRLEHARQSAARASYVGLLASGLAHEIRNPL
                     NAMSMNLQMLEEELQGVELAEHDDLGELLESTKSEIQRLESLVNNFLAYARPSPPRLA
                     SRNLNGVVREVLKFLEVDFRQSKVELRSELEPLLPDTEIDDTQFKQALLNLLVNARQV
                     LSAGGVVTVRTRAGARGEIVLEVEDDGPGIPREMRERIFEVFYSSRGGGTGLGLPIAR
                     QIIERHGGTIELDSEEGRGTRFTIHLPRHHARGEPASPPAERGPEAATR"
     gene            6325..8388
                     /gene="ligA"
                     /locus_tag="VD788_16900"
     CDS             6325..8388
                     /gene="ligA"
                     /locus_tag="VD788_16900"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXV77990.1"
                     /translation="MSRTDKRSAVRRADELKRLIAHHRKRYYVDDDPELSDAEYDALE
                     HELADIERRWPELVTADSPSVRVGGEPAEAFPTFHHPTPLLSLDNVTSEREIEEWEKR
                     LSRALGEARATFVVEPKVDGLSIAIHYRDGVLERGVTRGDGRIGEVVTGNVRTIRSIP
                     LRLARPVAALEARGEIFMARSAFRELNRRREQQGAPAFANPRNAAAGAVRLLDPRVTA
                     ERKLDCYFYALVSIEGDPAGEAKVDELRQDESLALLRDLGLKPNPLNRSCDDLVAVRA
                     AAERIRFARAELDYEIDGVVIKVNEPELRRRAGHTSKFPRWAVAFKFPAQQATTRVRE
                     IVVQVGRTGKLTPVAELDPVPLAGTTVSRATLHNEDEVARKDVRVGDTVLIEKAGEII
                     PQVVKTVADKRPSGARRFTMPSRCPVCGSEAVRAEGEVARYCSNVACPAQSREKLLHF
                     ASRAGMDIQGLGEALVEQLVAQELVRDVADLYSLDAERLAELERMGPKSAANLVSQIE
                     ASKAGALERLVYGLGIRHVGERAARILASRLGSIESLGRATTEELEALDEIGPKTAES
                     VRTFFAQPANLELLDRLARAGINPEATAHERTVTRSLDSPFSGKTVVLTGSLPHHTRD
                     EAKAAIESRGGRVTGSVSAKTDLVVAGEAAGSKIDRARTLGIRVIGPEEFERLLSGGE
                     RVRTR"
     gene            8412..9809
                     /locus_tag="VD788_16905"
     CDS             8412..9809
                     /locus_tag="VD788_16905"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV77991.1"
                     /translation="MSDKATIIVIEDDQANRRSLVRALSREGYEVTAFSAAEPALQHL
                     RENRQVNLVVTDLMLPGTDGFGVLEGARAVDPDVGVLMITGHGSVESAVEAMKRGADD
                     YLTKPVNLEELRKRVSTLVQKAQLSRRVDELEKRLGEKFGQLIGGSKPMEALFRQMDL
                     VSAARSNVLIVGESGTGKELVANALHEHSPRKDKRFLPINCAAIPSEILESELFGHER
                     GAFTGATSRKIGKFELADGGTLFLDEIGELPLEMQVKLLRVLEQREFMRVGGAETISV
                     DIRLIAATNQDLEVAVEQGRFRSDLYYRLKVVTLRIPPLRERREDIPRLAIHFLEKFA
                     GENGRPGMRFAPDAMRALVAAPWEGNVRELKNLVESLVVLSPVDEIRLEDLPAEYGEP
                     AADRDEEPAGAEQGGRPEPRDLTMEEIERRAILAALDKTKGNRTQAAEILGIGLRTLQ
                     RKLKEYRLAGLADGK"
     gene            9938..10351
                     /locus_tag="VD788_16910"
     CDS             9938..10351
                     /locus_tag="VD788_16910"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV77992.1"
                     /translation="MVPLSRHRYTVLVVDDDPSVLSTYRRLLTRAGYRTLVEQDPVRV
                     LEDRYEDRVPDLILLDYRMPGMDGLTLLANLRQRECSARCILLSAYLNDDVRTQARLL
                     GVDRVLEKPVDACTLREVIGELLPVTGPRAAGIAG"
     gene            10451..12031
                     /locus_tag="VD788_16915"
     CDS             10451..12031
                     /locus_tag="VD788_16915"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXV77993.1"
                     /translation="MNSRTFGFVSLMAVIGVSIVFGMILGGKLNAPQVSFAAPPGAPP
                     VDLAPAVTGLGGLVDFADVVERAMPAVVSVRSAELPGESRAEDDPEPDAPRQRGPEEW
                     FFRFFGDPDDPSPQRPPGQPRIGEGSGFIISTDGYLLTNNHVVETADDITVGLTNGRE
                     YKAKVIGTDPSIDLALLKIESEGESLPTLPLGDSSDLRVGEWVIAIGNPLEFEQTVTV
                     GVLSAKERRVAIGSTDAGVVSFLQTDAAINFGNSGGPLLDSRGNVIGINTAIRRANFA
                     EGIGFALPIDHARLVMDQLRQRGYVKRGYIGITMNPTGIDEEAREYLGLPDGFGVLVE
                     AVTGGGPADQAGIQTGDVIRKVDGEVVKDNLDLIGKIAARQPGDSVKIELFRKQRGRG
                     ETVRVEAELSDREEGLASATGRPTRPAQPVPEPEVRESSGLGMTVTELTDGMRERLSL
                     DEDAAGVLITDVEFNSQAADKGIGPDMVVTSIDNTSVSTVAEWESRIDQLVPGDVVKL
                     HVLAGGRDFTVFLRVPER"
     gene            12101..13516
                     /locus_tag="VD788_16920"
     CDS             12101..13516
                     /locus_tag="VD788_16920"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV77994.1"
                     /translation="MALEHKARVLVVDDEADIRASLKMILEYEGMRMIEAASGPEALD
                     RVRDDDPDAVLLDIKMPRVDGLEVLAQLGKTRPDLPVVMISGHGTISTAVEATRLGAF
                     DFMEKPLERDRVLLVLRNALERRRLVQTVREYRLSFEERFRLVGGSDSLAQIEQAIAR
                     IAPTKASVLITGESGTGKELLARAIHGNSTRADKPFVKVNCAAIPEELIESELFGHVR
                     GSFTGATKDQAGKFVRADGGTIFLDEVGDMSLKTQAKVLRVLQDGDVEPVGAAATLTV
                     DVRVVAATNKDLPVEIEAGNFREDLYFRLNVVPLHLPPLRERSEDVPPLVEYFAKSYC
                     RDNNYREKRFTRAALAALSRLPWRGNVRELRNAVERLIIMTPSDTIDEANLPAGLGLA
                     LGGPHGSVVTSAAGSLVIPHRGVGLQEFKDRAERAYLEAQLEANDWNIAATAKAIGTP
                     RSNLYKKLEAYGLGREKDGRR"
     gene            13513..>14066
                     /locus_tag="VD788_16925"
     CDS             13513..>14066
                     /locus_tag="VD788_16925"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HXV77995.1"
                     /translation="MNGGRDVVVVAGARTPFVKAGTLLARTSAVELGRIAVREAIEQA
                     DLDPAELDEVIVGNIAGPADAANIGRVISLMAKVPRAVPAFTVNRNCASGLECVVEAA
                     YRIRSGDADVVVAGAVESMSQIPLLFSPDAQRVWTRLARARGLLGRLAAIAAFRPRHF
                     KPTAALLQGLTDPVSGLNMGETAEL"
BASE COUNT         2249 a         4677 c         4934 g         2206 t
ORIGIN      
        1 cgcagcgagt gcttcaccgc acggtcctcg agcgagtggt acgagatgac cgccaggcgt
       61 ccgccgcacc ggagcaccga ggtggcgtcg gcgaccagac gctcgaggtc gtcgacctcg
      121 cgattgaccg cgatgcgcag cgcctggaag gtgcgcgtcg ccgggtggat gcgatagcgc
      181 cgggcggccg ggccgagcac gcgcgcgacg agctcggcga gctgacgcgt cgtctcgagc
      241 gggcggcgaa cgcgctcggc gacgatcgcc cgtgcgatcg cccgtgcgcg ccgctcctcg
      301 ccgtaacgcc agagcagccg gcgcagctcg tcctcgccgg cgccggccaa caactccgct
      361 gccgtcggtg ccgactgcgg gtccatccgc atgtcgagcg gcccgtcctc gcggaaggag
      421 aatccgcgct cggggctcgc gagctggagc gacgataggc cgaggtcgag cagaaccgca
      481 tcgacgcgct cgaccgcggc ggctcggagc agccggagca agtcggtatg gttgccgcgt
      541 acggcggtga acgccgggcc gaaccgcgcg aggcgccccg tggcgaccac gagcgcctgc
      601 gggtcgcggt cgatgccgat caaccgtccg gacggaccga tccgctcgag cagcgcgtgg
      661 gcgtgacccc ccggaccgag ggtcgcgtcg acgacggtgg agcccgcgcg gacggccagc
      721 cagtcgatcg tctcgcgcag caggacgggt tcgtgcaccg actccggtgt catcaccgtc
      781 tcctcgccgc tcgcgacggc gatcatcggc cgacccccgt catcagaacc ccatcgacgc
      841 caactcggcg aggtccgcgt cggtcagcgg gtcgcgctcg aggcgctctt cgaactgtgc
      901 gcggttccag acttcgagga agttctgctg cccgagcacg gcgacctcgc cgtcgatcgc
      961 ggcacgctcg cgcaacagcg ggtggatcag gatgcggccc tgcgggtcca tcgcggcggc
     1021 ctgcccgaag aagttgaggg acgtgcggag cttcatcacc gccggcttga gggtcgaggc
     1081 tcccatcagc cgctgctcga gcgaggagta gacttgcatc ggatacaggc gcacggagtc
     1141 gccgcgtagg ctcgtgacga agaactcgtt gccgtactcc ggctcgatga cggcacggaa
     1201 ggaggccggg agtttcaacc gccccttctc atcgaccttt gcgagagcgt ttccgcgaag
     1261 cataagattt cggtccgctc gggcaatgaa ataatgaacg gcccgcccag accgccaagc
     1321 cgtgccactt gtcgggggag cgggtcgccg tcacgttcgt tacctggagt tgtcaagcgg
     1381 agcggataca aaggccactc tactccactt tggtccacca gcccccgaat catagggagc
     1441 gatgcgcgtg gtgtcaacga ctctgatgaa cgaaattccg tgttcctgtg gcgaaagcag
     1501 agcgatagcc cgacgcggtg gatcgggcgt tcgcagcgct tcgcggaggt gagcccatga
     1561 acgatttgag aacgatctgc gcgcgctcgg cgcgccacgc gccggctgtc ggccggccgc
     1621 tcgccccggt gatcgtcccg tcgtcgacct tcggtttcga cacgctggcg gacgtcgatc
     1681 gttactacga cgcgggcgac ggctatctgt actcgcgcta cggcaatccg tcggtcgagc
     1741 gtgtcgagaa cctgttggcc gagctcgagg gcgcggaggg cgccgtctgc ttctcgtccg
     1801 ggatgggtgc gatcacgacc gccctgtcgg cggtcacgcg cgccggcgat cgggtcgtcg
     1861 cccaacgcga gctctacggg ggcacgagcg agtacctccg cggcgtgctg ccgctcggcg
     1921 gcgtgaaggt cgactggatc acgaccgacg agttggcgcg cctcgaaccg gcccggctcg
     1981 tcggcgcccg tgcgctctac ctcgaatcgc cgaccaaccc gacgctgcgc atcgtcgacc
     2041 tcgagcgcgt ggcgacgatc gcccgcgagg tggccgtggt caccctggtc gacgcgacct
     2101 tcgccacccc ggctctcgag cgcccgatcg agcgcgggat cgacctcgtc gtgcacagcg
     2161 cgaccaagta cctcggcggt cacggtgacc tgatcggcgg ggtcgtagcc ggccgcggcg
     2221 agtcgatcgg cgcgatacgc gagcggcgga ggatcaccgg ggcgacgatg gacccgtttt
     2281 ccgccttcct gctgcatcgc ggcctgcgca ccctggccgt acgcgtcgag gcccactgtc
     2341 gcggcgccgc acgggtggcg gcccatctgg agcgtgcggc cggggtcgag cgcgtgatct
     2401 accccgggct cgcgtcgcat ccggaccacg agctggcgcg acgccggatg cccggctacg
     2461 gcggcatggt ctccttcgtc atctcgggcg gcgcaccggc ggcggagcgg gtccacgacg
     2521 cgctgacctt gttcgccaag gcgggcagcc tgggcagcat cgaatcgctg gtgtcgatcc
     2581 cggctcggat gtcccaccgg cacctcggcg ccgaggcccg gctcgaggcc ggcatcccgg
     2641 acggactgtt gcggttgtcg gtcgggctcg agtcgcccga ggatctgatc gcggacctcg
     2701 accgggcgct ggcggccgcg ggctgagtcg atcggcctcc gcccgataaa aaggaagggc
     2761 ccgcctcgcg gcgggccctt cgtcaggaga ctacggacat gatcagcgtg tgggtgggta
     2821 gatcttcttg tcgaacggtg cgaagtagcg ggaagtaccg ctcgcgccgg aatcgatccc
     2881 ctgattctcg tcggacggcg gcgggctgcc gtcctcgtgg ccgaccgggt cgctaccgtc
     2941 cgtcagcccg agcgtcgacg tcgtgccgtc gcccggcagc ggaagctgga acaggtggat
     3001 ctgccggtcg atctggaacg ccacgaccaa gcggtcttcg gtgacgagca agctcggcgt
     3061 ttcggcgccc gaggccaacg gagtgacggg cagcggcgtg ctccagtcgg tccggccggc
     3121 cggccggatc gcgagcatca cccgaccctc gtcgcgatcg gtgtacgcca ggaagacgtt
     3181 gccgtgggcg tcggcggcga ccaccggctc gatctggtct cgctcgtcgt cgcgtccgaa
     3241 gaacaccggc tcggaccagt ccccgtcagc ctgcgccgtc gaaactgcga tgtcgaaccg
     3301 ggcgccgttc cggtaggccc aagtcgcgat cgtcgtttgg gaggcgagta cttcgacttg
     3361 gtggatacgg ccgtccatcg agcgcggcgc gtagcgaggc ggcagcccac gtggtccgtc
     3421 cgcgagcgca acggtcccga gggcgacaat cgtcgaaacg gcggcaaaga tcgtcagctt
     3481 tttatgcatg gggtttcccc ctttctacgt agtcgtcgaa cgcatcgacc tcgacggtcc
     3541 gtcgttcgag actcgagcgc agcgccttca ggcggccgaa cgcgatcttc tcgcgcgtgg
     3601 gattctcggt ttgaatcgcc atcttccatt cgtagaaggc gttgaagaac aagatgtctg
     3661 gctgcttctc accgttgtga tgcgcgaggg cgtcggattc gaggaaacac cctcgagccc
     3721 gtcgcaggtc gtcctggcga tagtacgcct cgccgagatt gctccaggcg tgggcctcga
     3781 gtcgccgatg acccgcattg cgcgcttcgt tgagcgcctc gcgcagtagg cggatgccct
     3841 cgcgatactt gcccagtgcg acgtagcacg tgccggtgtt gatacgaacg cgattggcct
     3901 cgaagtcgtt gccgcagcgc gcgaacagct tgccggcctc gcggaagcgc tcgatcgcct
     3961 cggtcggcgc accacgctgg tgcaggacgt ggccgaacag gttgagcgcc ttggccgcct
     4021 cgagcggtag cgaagcgttg gcggcgatct gatatgcgcg atcggcttcc atctcgctga
     4081 ggaagtggtc gccctggtcg gtgtggatca acgcgagcgc gagcagggcg cgtgcctgga
     4141 gcacggggct gaggcgctcg gacagccgga gcgcgctacg cagctcctgc tcggcgagcg
     4201 ccaattttcc cagccggttg agcacggcgg cctggttgat ccgggctcga ccgagctgct
     4261 cttgcgtctc cgagtcttgc ggtccgtccg acagcagctc gatcgcccgc tcgaacaacg
     4321 cgaaggagag acccgtgtcg ccggccttga gcgcatccag gccacgctcc tgcagctcgg
     4381 cggcatcgtt ggtgtccggc ttgagcgcct cgaatgactc gaggtcgacc acgtcggaga
     4441 aggactggat cgacacgttg aaaacgttgg cgaggatgcg tagcttgtcg aacgacgggt
     4501 agcagacacc cttctcgtag cggctgagct gcgaattgtc gatctcgccg ccctcggccc
     4561 gcgctcgctc ctcgactcgc ctcagggaat agccatagcc cgtgcgcagc ttccgcaggt
     4621 agctcccgag cttgacgctt cccttcagcg ccgctgcctg tggttgagat gccatgcgcc
     4681 ctcctgcctc ggctcttgcc caccggatca cccggtcggc cacccgtccc cggatcggat
     4741 cacatgtcgc cgcattctgg actaggcata gaaacgttta ctgtttagta accaccgcgt
     4801 caagtcggcg tcattttcca ggaattttct gctgtgaatt tgcagcacgc ggaatcacga
     4861 tctcgcggcg aatccccgga tagaatacct tgaatcggta tttttccggg ggaaaacggt
     4921 gccggaagcc gtttccgtcg atcgtggcga cttacgccct acgttcgctt actcgatttc
     4981 gagggaagcg gtccaggcgt tcgtgctatt cggcttcctg ctcgcggtct tggtggcgat
     5041 cacgctcgat ctcgcttttc gccagctcag cgtatcggtg ctcgagcgac ggctcgatct
     5101 ggggatggtc gaggcgcggg acatcgcgcg gatcgtctcc gacgtcggcc gcgacgattc
     5161 cggtatcgat ttttcgctgg tgcgcgagcg gggcgactac ctgcaagacg tgatccatcg
     5221 acgcatctcg atgcgggtct tcgtgcacca cgtcgaggtg gtcgaccggt tcggcatcca
     5281 gcagctgttc gtcgccaaca ccgccttcca gcacgaaccg ccgcgggtcg ttcccgacct
     5341 gtcgttcccc gaagactggc cgaagagctt cgaacagatc gtgcagtggc cgctgacccg
     5401 gtcggagggc atggtcaagc tcggggtctc gtcgcagccg atcctcgacg agctttccat
     5461 gctgcggtcg tcgctccgca agaaattgat gctgggcgcc gcgctggcgc tcgcgttgct
     5521 ggtggccggt ttcttctacg tgctgtacct gctgcggaag aaccggcggc tcgagcacgc
     5581 gcggcagtcg gccgcgcggg cctcctatgt cgggctgctc gcatccggcc tggcgcacga
     5641 gatccgcaac ccgctcaacg cgatgagcat gaacctgcag atgctcgagg aggagctgca
     5701 gggcgtcgag ctcgccgagc acgatgacct cggcgagttg ctcgaatcga ccaagagtga
     5761 gatccagcgt ctggagagcc tggtcaacaa cttcctggcc tacgcgcgcc cctcgccgcc
     5821 gcgcctcgcc tcgcgcaatc tgaacggggt cgtgcgcgag gtgctcaagt tcctcgaggt
     5881 cgactttcgc cagagcaagg tcgagctgag gtccgagctc gagccgcttt tgccggacac
     5941 ggagatcgac gacacgcagt tcaaacaagc gctgctgaac ctgctggtca acgctcgcca
     6001 agtgctgtcc gccggcggag tcgtcacggt gcggacacgc gccggagcgc gtggggagat
     6061 cgtgctcgag gtcgaagacg acgggcccgg catcccgcgc gagatgcggg agcggatctt
     6121 cgaggtcttc tattcgagcc gcgggggcgg caccggactg ggactgccga tcgcccgcca
     6181 gatcatcgaa cgccacggcg gcacgatcga actcgacagc gaggaggggc gcggcacccg
     6241 cttcacgatc cacctgccac ggcaccacgc gcgcggcgag ccggcgagcc cgccggccga
     6301 gcgcggcccg gaggcggcga cgcgatgagc cggaccgaca agcgctccgc cgtccggcgg
     6361 gcggacgagc tcaagcggct gatcgcccac caccgcaagc gctactacgt cgacgacgac
     6421 ccggagctgt cggatgccga atacgacgcg ctcgagcacg agctggccga tatcgagcgg
     6481 cgctggccgg agctggtcac cgccgactct ccgtcggtgc gcgtcggcgg cgagccggcg
     6541 gaggcgttcc ccaccttcca ccacccgacg ccgctgctgt cgctcgacaa cgtcacctcg
     6601 gagcgggaga tcgaggaatg ggagaagcgc ctgtcgcgtg cactcggcga ggcccgagcg
     6661 acgttcgtcg tcgagccgaa ggtcgacggc ctgtcgatcg ccatccacta tcgcgacggg
     6721 gtgctcgaac gcggcgtgac ccggggtgac ggccgcatcg gtgaggtcgt cacgggcaac
     6781 gtgaggacga tccgctcgat cccgctgcgt ctcgcccgac cggtcgcggc gctcgaggcg
     6841 cgtggcgaga tcttcatggc gcgcagcgcc tttcgcgagc tgaaccggcg acgcgagcag
     6901 cagggcgcac cggcgttcgc caacccgcga aacgcggccg ccggcgccgt ccgcctgctc
     6961 gacccccgcg tgaccgccga gcgcaagctg gactgctact tctacgcgct ggtctcgatc
     7021 gagggcgacc cggccggcga ggcgaaggtc gacgaactgc gccaggacga gtcactcgcg
     7081 ttgctgcggg atctcggtct caagcccaac ccgctgaatc ggagctgcga cgacctggtg
     7141 gccgtgcgcg cggccgccga gcggatccga ttcgcgcgcg cggagctcga ctacgagatc
     7201 gacggcgtcg tgatcaaggt caacgagccg gagctgcgcc gccgcgccgg ccacacgtcg
     7261 aagttcccgc gctgggcggt cgcgttcaag ttcccggcac agcaggcgac gacgcgcgtg
     7321 cgtgagatcg tggtgcaggt cggccggacc ggcaagctca cgccggtggc cgaactcgat
     7381 ccggtgccgc tcgccggcac caccgtctcg cgcgccacgc tgcacaacga agacgaggtc
     7441 gcacgcaagg acgtgcgggt cggcgacacg gtgctgatcg agaaggccgg tgagatcatc
     7501 ccccaggtgg tgaagaccgt cgccgacaag cgcccctcag gcgcccggcg gttcaccatg
     7561 ccgtctcgct gtccggtttg cggctcggag gccgtgcgcg ccgagggcga ggtcgcgcgc
     7621 tactgcagca acgtcgcctg tccggcccag agccgcgaga aactgctgca tttcgcctcc
     7681 cgcgccggga tggacatcca gggactgggc gaggcgctcg tcgagcaact cgtggcgcag
     7741 gagctggtgc gggacgtcgc cgacctgtac tcgctcgatg ccgagcgctt ggccgagctc
     7801 gagcggatgg gaccgaagtc ggccgccaac ctggtgagcc agatcgaggc ctcgaaggcc
     7861 ggcgcgctcg agcggctggt ctacggtctc ggcattcgcc acgtcggcga gcgcgcggcg
     7921 cgcatcctgg ccagccgtct ggggtcgatc gagtcgctgg gtcgcgcgac gaccgaggag
     7981 ctcgaggcgc tcgacgagat cgggccgaag accgccgagt cggtccgtac gttcttcgcg
     8041 caaccggcca acctcgagct gctcgatcga cttgcgcgcg cggggatcaa cccggaggcc
     8101 acggctcacg aacggacggt gacccgttcg ctcgattcgc cgttcagcgg caagacggtg
     8161 gtgctgaccg gcagtctgcc gcatcacacg cgcgacgagg ccaaggcggc gatcgagtcc
     8221 cgcgggggcc gggtgacggg cagtgtcagc gcgaagacgg acttggtggt ggcgggcgag
     8281 gcggccggat cgaagatcga ccgggcccgc accctcggca tccgcgtgat cgggcccgag
     8341 gagttcgagc ggctgctgtc ggggggcgag cgcgtgagga cgcgctagaa ttgcccggcg
     8401 ggacgaatcc gatgagcgac aaggcaacga tcatcgtcat cgaggacgat caggcgaacc
     8461 gtcgcagcct ggtccgcgcg ttgtcgcgcg agggttacga ggtgaccgcg ttctcggcgg
     8521 cggagccggc cttgcagcac ttgcgcgaga accgccaggt caatctggtc gtcaccgacc
     8581 tgatgctccc gggcaccgac gggttcggcg tgctggaggg tgcccgcgcg gtcgatcccg
     8641 acgtcggcgt gctgatgatc accggtcacg gctcggtcga atccgccgtc gaggcgatga
     8701 agcgcggggc ggacgactat ctgaccaagc cggtcaacct cgaggagctg cgcaagcgtg
     8761 tttcgacgct ggtgcagaag gcccagctgt cgcggcgcgt cgacgagctg gaaaagcgcc
     8821 tcggcgagaa gttcggccag ctgatcggcg gctcgaagcc gatggaagcg ctgttccgcc
     8881 agatggacct ggtctcggcc gcgcgctcca acgtgttgat cgtcggcgaa tcggggacgg
     8941 gcaaggaact ggtggccaat gcgctgcacg agcattcgcc acgcaaggac aagcggttcc
     9001 tgccgatcaa ctgcgcggcg atcccgtcgg agatcctcga gtccgagctg ttcggccacg
     9061 agcggggcgc cttcaccggc gccacgtcgc gcaagatcgg caagttcgag ctggccgacg
     9121 gcgggacgct gttcctggat gaaatcggcg agcttccgct cgagatgcag gtgaagctgt
     9181 tgcgcgtgct ggaacagcgc gagttcatgc gcgtcggcgg tgcggagacg atcagcgtcg
     9241 atatccgtct gatcgcggcg accaaccagg acctcgaggt ggcggtcgag caggggcgat
     9301 tccgctcgga tctgtactat cggctgaagg tggtcacgct gcgcatcccg ccgctgcgcg
     9361 agcgtcgcga ggacatccca cggctggcga tccacttcct cgagaagttc gccggcgaga
     9421 acggccgtcc cggcatgcgc ttcgccccgg acgcgatgcg cgccctggta gcggcgccct
     9481 gggagggcaa cgtccgcgag ttgaaaaacc tggtcgagag cctggtcgtc ctctcgccgg
     9541 tggacgagat ccgcctcgag gacctgccgg ccgagtacgg cgagccggcc gcggaccgcg
     9601 acgaggagcc ggctggagcg gagcaaggcg ggcggcccga gccgcgggac ctgacgatgg
     9661 aggagatcga gcggcgggcg atcctcgccg ccctggacaa gacgaagggc aatcgcaccc
     9721 aggcggccga gatcctcgga atcggcttgc gcacgctgca gcgcaagctc aaggagtacc
     9781 ggctggccgg tctggcggac ggcaaatgac ggccgattcg cgccagtttg acgtccggca
     9841 acggcccgcg ggcggtcgag ggcgccgcca tgacatggat gatcggccga acgccccgga
     9901 aacgtcgggc acgtcgcttg cttttggttg aggtggcatg gttcctcttt cccgccatcg
     9961 gtacacggtc ttggtcgtcg acgacgaccc gtcggtcctg tcgacctacc gccgtctgtt
    10021 gacgcgggcc ggctaccgca cgctggtcga gcaggacccg gttcgcgtgc tcgaagaccg
    10081 ctacgaggac cgcgtaccgg atctgatcct gctcgactac cggatgccgg ggatggacgg
    10141 gctgacgctg ttggccaacc tgcgtcagcg ggaatgctcc gcgagatgta tcctgctgtc
    10201 ggcctatctc aacgacgacg ttcggactca ggccaggttg ctcggcgtcg accgagtcct
    10261 cgaaaagccg gtcgacgcct gtacgctgcg cgaggtgatc ggcgagttgc tgccggtgac
    10321 cggcccgcgg gctgcgggaa tcgccggttg aaggcgaccc ccgccccccg gctcgcgagt
    10381 cccgatcgaa ctgtggaaca ctgatgggca tagacgacag gggaccagca caggcggagg
    10441 accggtcgcg atgaacagcc ggacattcgg tttcgtgtcg ctgatggccg tgatcggcgt
    10501 cagcatcgtt ttcggcatga tcctcggcgg gaagctcaac gcgccccagg tcagcttcgc
    10561 ggcgccgccg ggcgcgccgc cggtcgatct cgcgccggcc gtcaccggtc ttggcggtct
    10621 cgtcgacttc gcggacgtcg tcgagcgtgc gatgccggcg gtggtcagcg tgcggagcgc
    10681 cgagctgccg ggcgagagtc gcgccgagga cgatccggag cccgacgccc cacgtcagcg
    10741 cggaccggag gaatggttct ttcgtttctt cggcgatccg gacgatccct cgccgcagcg
    10801 ccctcccggc cagccgcgca tcggcgaagg gtcgggcttc atcatctcga ccgacggcta
    10861 cctgctgacg aacaaccacg tggtcgaaac ggccgacgac atcaccgtcg gtctgacgaa
    10921 cgggagggag tacaaggcaa aggtgatcgg gaccgacccg tcgatcgacc tcgcgctgct
    10981 caagatcgag agcgaaggcg agagcttgcc caccctgccg ttgggtgatt cgagcgacct
    11041 gcgcgtcggt gaatgggtca tcgccatcgg caacccgctc gaattcgaac agaccgtgac
    11101 cgtcggcgtc ctgtcggcca aggagcgtcg cgtcgcgatc ggcagcaccg acgccggcgt
    11161 cgtcagcttt ctgcagaccg acgcggcgat caacttcggc aactcgggcg gtccgctgct
    11221 cgacagccgg ggcaacgtca tcggcatcaa caccgcgatc cgtcgcgcga acttcgccga
    11281 gggcatcggc ttcgcgctgc cgatcgacca cgcccggctg gtgatggatc agctgcgcca
    11341 gcggggctac gtcaagcgcg ggtacatcgg catcacgatg aacccgaccg gcatcgacga
    11401 ggaagcgcgg gagtacctcg ggctgccgga cggcttcggc gtactggtcg aagcggtgac
    11461 gggcggcggc ccggccgatc aggccggcat ccagaccggc gacgtgatcc gcaaggtcga
    11521 cggcgaggtg gtcaaggaca acctggatct gatcggcaag atcgccgcgc ggcagccggg
    11581 cgactcggtc aagatcgagc tgttccgcaa gcaacgcggt cgtggcgaga cggtgcgcgt
    11641 cgaagccgag ctgagcgatc gcgaggaagg gctcgcctcg gccaccggcc gaccgacacg
    11701 accggcgcaa ccggtgccgg agcccgaggt gcgagagtcg tctgggctcg ggatgacggt
    11761 gaccgagctg accgacggga tgcgcgagcg gctgtcactc gacgaagacg ccgccggcgt
    11821 gttgatcacc gacgtcgagt tcaacagtca ggcggcggac aaggggatcg gccccgacat
    11881 ggtggtcacg tcgatcgaca acacttcggt gagcacggtc gcagagtggg agagtcggat
    11941 cgaccaactc gtgcccggcg acgtggtcaa gctccacgtg ctcgccggcg gccgcgactt
    12001 cacggtcttc ctgcgcgtcc ccgaacgtta accgcgctgg tcggtatgtt atgctcccga
    12061 tcccgcggac ttcccgcgcg gtccggagtt cgtccgaccg atggccctcg aacacaaggc
    12121 ccgcgtcctg gtagtcgacg acgaggccga catccgcgcc tcgctcaaga tgatcctcga
    12181 gtacgaggga atgcggatga tcgaggccgc ctccggcccg gaggcgctcg accgggtgcg
    12241 cgacgacgac cccgacgcgg tgctgctcga catcaagatg ccgcgtgtcg acggtctcga
    12301 ggtgctggcc cagctcggca agacccgccc cgacctgccg gtggtgatga tctccggcca
    12361 cggcacgatt tcgacggcgg tcgaggccac gcggctcggc gccttcgact tcatggaaaa
    12421 gccgctcgag cgcgatcgcg tgctgctcgt gctgcgcaac gcgctcgagc gccgcaggct
    12481 cgtgcagacc gtacgagagt accggctgag cttcgaagag cgcttccgcc tggtcggtgg
    12541 ttccgacagc ctggcacaga tcgagcaggc gatcgccagg atcgcaccga ccaaggcctc
    12601 ggtgctgatc accggcgagt cgggaaccgg caaagagctg ctcgcgcgcg cgatccacgg
    12661 taacagcacg cgcgccgaca agccgttcgt caaggtcaat tgcgccgcga tccccgagga
    12721 gctgatcgaa tcggagctgt tcggccacgt tcgtggctcg ttcacggggg cgacgaagga
    12781 ccaggccggc aagttcgtgc gcgcggacgg cggcacgatc ttcctcgacg aagtcggcga
    12841 catgagcctg aagacgcagg ccaaggtgct gcgcgtgctg caggacggcg acgtcgaacc
    12901 ggtcggtgcc gccgccacgc tgaccgtcga cgtgcgtgtc gtcgccgcga ccaacaaaga
    12961 cctgccggtc gagatcgaag ccgggaactt ccgcgaggac ctctacttcc gcctcaacgt
    13021 ggtgccgttg cacctgccgc cgctgcgcga gcggagcgag gacgttccgc cgttggtcga
    13081 gtacttcgcc aagagctact gtcgtgacaa caactaccgc gagaaacgct tcacccgcgc
    13141 cgcgttggcc gcgttgtcgc ggctgccgtg gcgcggcaac gtccgcgagc tgcgcaacgc
    13201 cgtcgagcgc ttgatcatca tgacgccgtc ggacacgatc gacgaggcga acctgccggc
    13261 cgggctgggc ctggcgctcg gcgggcccca cggcagcgtg gtgacctcgg ccgccggctc
    13321 gctcgtcatc ccacaccgcg gggtcggcct gcaggaattc aaggaccgcg ccgaacgggc
    13381 ctacctcgag gcccagctgg aagccaacga ctggaacatc gccgcgaccg ccaaggcgat
    13441 cggcacgccg cgttcgaacc tctacaagaa gctggaggcg tacgggctcg gccgggagaa
    13501 ggacgggcgc aggtgaacgg tggccgcgac gtggtggtgg tcgccggggc gcgcacaccg
    13561 ttcgtcaagg cgggaacgct gctggcgcgc acgtcggccg tcgagctggg gcggatcgcc
    13621 gtgcgcgagg cgatcgagca ggccgacctc gatccggccg agctggacga ggtgatcgtc
    13681 gggaacatcg ccggcccggc cgacgcggcc aatatcggcc gcgtgatctc gctgatggcc
    13741 aaggtgccgc gcgcggtgcc ggcgttcacc gtcaaccgca actgcgcatc ggggctcgag
    13801 tgcgtcgtcg aggccgccta ccggatccgg tcgggtgacg cggacgtcgt cgtcgccggc
    13861 gccgtcgagt cgatgtcgca gatcccgctg ctgttctcgc ccgacgccca gcgggtctgg
    13921 acgcggctcg cgcgggcccg tggtctgctc ggccggttgg cggcgatcgc ggctttcaga
    13981 ccgcggcact tcaaaccgac cgcggccttg ctccagggtc tgaccgaccc cgtgtccggt
    14041 ctcaacatgg gcgagaccgc ggagct
//
LOCUS       DASPGN010000587        11007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_110689, whole genome shotgun sequence.
ACCESSION   DASPGN010000587 DASPGN010000000
VERSION     DASPGN010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 11007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11007
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110689"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VD788_16930"
     CDS             <1..528
                     /locus_tag="VD788_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77996.1"
                     /translation="LPVAEGRMRALYRLGRPDKAAEVGRAMLDHPSGSADDRAVLARI
                     AALLERIRLDRGEPEGAVRRRAIRALVDDPANVELLRLVRDTHGRLSPDARHLRLLLH
                     GRCEAATGDGDGYYVSYRVVADDEREAAGFVAEIEALVTGCDELQVEECEALEPAAAD
                     AKGVYWYGGRVYYTE"
     gene            596..1129
                     /locus_tag="VD788_16935"
     CDS             596..1129
                     /locus_tag="VD788_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77997.1"
                     /translation="MRASAWVVSCLAAIAIGAAFAQPAPDTRFTADGERQILEMSDWL
                     EKHPLAADRSEVFGRVLEWWVEAPQLTLNWCPAILREAQNDKTNSLVTTQAVVGAGAH
                     LIRHRDEADVVGIGLAGVESALRAYESAVEQDPSYRDPAYDRLIERRQADGLSRYVTD
                     KLKECEGDAERPYRDVT"
     gene            1294..1446
                     /locus_tag="VD788_16940"
     CDS             1294..1446
                     /locus_tag="VD788_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV77998.1"
                     /translation="MSALEIAFSISPLWLALARPTAFKVHTAFGEYTHLYLDRDERFL
                     CNYTSW"
     gene            1443..1865
                     /locus_tag="VD788_16945"
     CDS             1443..1865
                     /locus_tag="VD788_16945"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HXV77999.1"
                     /translation="MTASQVHYRALADSVLALHAAFVIFVVVGLALVLVGGARGWRWV
                     RNPWFRLAHLLAIGVVVLQAWLGVLCPLTTLEMRLRERAGDAVYRGSFIAHWLERLLY
                     YDAPAWVFVVCYTGFGLLVAGSWWWVRPGPFHDRSALE"
     gene            1890..2366
                     /locus_tag="VD788_16950"
     CDS             1890..2366
                     /locus_tag="VD788_16950"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV78000.1"
                     /translation="MKPVINLDELEIESHDRGSFGSRYGVISDRIGARKLGYNLTICP
                     PGKRACPFHNHHTNEEMFLILEGEGLLRFGDDEYPLRPNDVIACPPGNRDVAHQIINT
                     GSTELKYLALSTRERTEVVEYPDSDKIGVMVGPFGDQDLRAIFRAEQTVDYFDREE"
     gene            2477..3058
                     /locus_tag="VD788_16955"
     CDS             2477..3058
                     /locus_tag="VD788_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78001.1"
                     /translation="MAALAVGTPADEGQRFENATAGISLERPAGWVTASIQQVEANRK
                     RVRLPDDELQAAVEEHASAPLFVFTRYPEPHDDLNPSIQVILRPLGELAGLPPIEVMQ
                     AAVGPLQEIFGNFEFVEEIHPTEVAGYPAARMSATYTIENDEGRAFETVSKMWIVPRG
                     SFMFMLGMSGAIEDRDELEEIFERAIGSVEIAD"
     gene            3063..3500
                     /locus_tag="VD788_16960"
     CDS             3063..3500
                     /locus_tag="VD788_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78002.1"
                     /translation="MTTTRSATVVATIVLATCCGYVQRGSWTDDPRNWYRIFGEAKPD
                     EVTLVHSFYERLPGFRMRYRFYLAFEAPAEARERLVARLSLSPLPHDLAPTDAAGADP
                     ARPAWFAPGDPGDYRIWIDAPNRRLRMFVNPDTGATYLSDRRS"
     gene            3514..4269
                     /locus_tag="VD788_16965"
     CDS             3514..4269
                     /locus_tag="VD788_16965"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78003.1"
                     /translation="MAPVRTTGMLTRATLAAATDALAACDADLARIVERYGRPPLWSR
                     RPGFVTLLRIVLEQQVSLASARATYRRLAERIDPLTPETVIAGGSVALRAAGLTRQKT
                     AYCLDLAEAIARGALDLRGLGRVDDATVSARLTRIKGIGPWTADCYLLMALRRPDVWP
                     AGDIALASAVREVKGLRRRPTPETLTTIAEGWRPHRAAAARMLWHYYLSRRERKGGKD
                     DDQGRAHDVLHVGAGGAARIPARQVGVPTQRRR"
     gene            4241..4531
                     /locus_tag="VD788_16970"
     CDS             4241..4531
                     /locus_tag="VD788_16970"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV78004.1"
                     /translation="MGFPHSDVGDGWLIFDLPEADMGCHPADEGSEDGKPSGTCDISF
                     YCEDVVQTVKELEARGVEFVDEVENHGYGLVTHFSMPGGIVAQLYQPLYDKR"
     gene            4534..5115
                     /locus_tag="VD788_16975"
     CDS             4534..5115
                     /locus_tag="VD788_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78005.1"
                     /translation="MADERQPDLERLERGLVAIGRSPTDAGRVELIACRPREGERRVL
                     EQARLDVGDGLIGDDWKTRGSRRTTDGNAHPGMQLNVMNSRVIGLLAGDRARWPLAGD
                     QLYVDLDLSTANLPPGTRLAVGSALIEVTAEPHLGCRKFELRFGLDALKFVNSRQRRH
                     LNLRGINARVVRSGDVRLGDVVRKLPDLADARR"
     gene            complement(5112..5495)
                     /locus_tag="VD788_16980"
     CDS             complement(5112..5495)
                     /locus_tag="VD788_16980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXV78006.1"
                     /translation="MSKRIVSTKHAPAAVGPYSQGVIMDGWLWTSGQVALDPASGKLV
                     GSDAATQAERALENLARILAEGGSGLDRVVRTTVYLTSMDDFKPVNEVYARFFSEPYP
                     ARACVEVRRLPVGALVEIDAIARVG"
     gene            5581..6084
                     /locus_tag="VD788_16985"
     CDS             5581..6084
                     /locus_tag="VD788_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78007.1"
                     /translation="MMNEGKRLYAVLSLVALVGGAAAAGQYEANVRRDVAAYFDALRV
                     SDFPTLAGLTCPAVVDSVGGYGAFITSLQAMDRERAAQGIEWGEVTIESVSEPVVAGE
                     QLHVLIEVRQQIGSPSGKTKKKTYMLGVSEDAGDSWTFVDATKLDAANKQKLFPEFND
                     ALKLPKG"
     gene            6098..6430
                     /locus_tag="VD788_16990"
     CDS             6098..6430
                     /locus_tag="VD788_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78008.1"
                     /translation="MSWRDWDALWGALPVIAWILIIVLRGLKTDRARTARPGRPLSTP
                     GGFERAYEPIEPSFRGRVPTPPVPASESRRRAVRTIDLGQWIEDLASDRGPDDDERAP
                     SLRTDRDG"
     gene            complement(6436..6828)
                     /locus_tag="VD788_16995"
     CDS             complement(6436..6828)
                     /locus_tag="VD788_16995"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXV78009.1"
                     /translation="MGDGNKPPVGTVVWTDLTVDDAERLRDFYRRVVGWGVAPVEMGG
                     YSDFSMTAPASGDAVAGVCHARGTNEGLPAQWLVYITVENLDRSIAACEELGGTVVNG
                     PKSMGSHGRYCVIRDPAGAVAALIESLA"
     gene            6930..7292
                     /locus_tag="VD788_17000"
     CDS             6930..7292
                     /locus_tag="VD788_17000"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HXV78010.1"
                     /translation="MSEQTAAIPREARNWAMGAHMIALVGLLGNGLGFLVGPLILWLL
                     KRDAHPFINEQGKEAVNFQITMLGAILVCIPLLFVGIGILLMPLIGLYDVVMTIIAGI
                     KAADGTHWRYPLTMRLIA"
     gene            7397..7831
                     /locus_tag="VD788_17005"
     CDS             7397..7831
                     /locus_tag="VD788_17005"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HXV78011.1"
                     /translation="MRKILYWVGGVAVAAFVAIQFVPVDRANPPVEADLVAPDDVRQV
                     LRVACYDCHSFETRWPWYSHIAPVSWLVARDVRSARAELVFSEWERMSAREQAELREE
                     IWEEVEEGEMPLKAYRLMHAEARLGPDQLETLRRWAHQSQPN"
     gene            7851..8360
                     /locus_tag="VD788_17010"
     CDS             7851..8360
                     /locus_tag="VD788_17010"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HXV78012.1"
                     /translation="MGKKEKDKKKKKDKKKKKAKDKLVEQSLAADRATVEESETILEQ
                     IGPSFLEHGAHLVRATDLARVVDQRDELTERFGADRPLGRFRDEARLMLSLLQDFRAG
                     QYTDISYWSIAVMVFGFQYVLKPIDIIPDSLPAIGQLDDTVVMSHCLEMIQPELLDYK
                     VRMLTREES"
     gene            complement(8375..9322)
                     /locus_tag="VD788_17015"
     CDS             complement(8375..9322)
                     /locus_tag="VD788_17015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78013.1"
                     /translation="MSIFGAAMRGFGATLAAPRILVGLWLVSLLFALPFAALVGRAIG
                     ESIGASRVHEELRRGFDGGWYGEFAHRADGLAASFRPTVIGATAFYDNLEDWVAGTLF
                     VEYPEVVAAGAAWAVLWVFLLGGVLDRFARPAESAVRGRFAQAGGIYFFRFLRLALLS
                     GVLYLGIYLLHRLLLGWLEYLTRDATREWTVLWLTLALYLLTALLLTLVQMTFAYAKI
                     ATVVENRASIFPSALRGLRFVAGFPAKTLGLQFGLAAVSLLLLVVYSWLAPGPGQSGV
                     PAIALAFLAGQAHLFTKLTLRLTSYGAATALFKSLAPRS"
     gene            complement(9332..>11007)
                     /locus_tag="VD788_17020"
     CDS             complement(9332..>11007)
                     /locus_tag="VD788_17020"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HXV78014.1"
                     /translation="PGHEQAPLELLTASYYAAPDDGNPQDRTVFVVPLPFAVEPGGEV
                     QVELAWRARVPRTFARTGFRGDYFFFAHWFPKLGVFEEDGWNCHQYHNLTEYYSDFGN
                     YRVSMTVPAGWVLGATGREVERTAHDDGSETHVYEQHDVHAFSWTTSPDFVVREQRFE
                     RAGLPPVDIRLLLQPEHLAQAERHFHATAAGLEHYGTWYGPYPYGHVTVVDPAYGSDT
                     GGMEYPTFFTCGTRIFNPFGADSPESVTIHEVGHQFWYGVVANNEFEHAWIDEGLNTF
                     STIRTLDATYPPLELVQRYLAPPGEDSSRGFLPVSIDGVELSRFTRRLDRYREHAEVA
                     DLDGFTFEYYPDGGSSLSYDKTALWLATLERHLGWETLQTILSTFFERHRFGHATPDA
                     FLDTVEEFAGPMDWFFDQVYRDAVVFDYAVDSVHSAPVEAEGFTQRDGEMVRTRREGQ
                     GSDFRTEVMVRRVGGGVFPVDVVLTFEDGQRIRKHWSGEERSKLFVVERAAKIERAEV
                     DPDRVLMLDVDYTNNSRLREPRARLPAVKWASKWMIWFQDAMATFAYFI"
BASE COUNT         1854 a         3598 c         3853 g         1702 t
ORIGIN      
        1 ctgccggtgg ccgaggggcg gatgcgcgcg ttgtaccgtc tcggccggcc ggacaaggcg
       61 gcggaggtcg gacgggcgat gctcgatcac ccgtcgggtt ccgctgacga tcgggccgtg
      121 ctggcccgca tcgcggcgct cttggagcgg atccgcctcg atcggggcga gccggaaggt
      181 gcggtgcgcc ggcgcgccat ccgtgcgctc gtcgatgacc cggcgaacgt cgagctgctg
      241 cgactcgtcc gcgatacgca cggtcgactc tcgcccgatg cgcgccacct tcgcttgctg
      301 ctccacggca ggtgcgaagc ggcgacgggc gacggcgacg gttactacgt ttcctatcgg
      361 gtcgtggccg acgacgaacg ggaggccgcc gggttcgtcg cggagatcga agcgctggtg
      421 accggctgcg acgagctgca agtcgaggag tgcgaggcgc tcgagcccgc ggccgccgac
      481 gcgaagggag tctattggta cggcgggcgc gtgtactaca ccgaatgacc cggcgcggcc
      541 ggcccttggc cgcggattcg cagtccgctt acaatcgctt cagaggtggt ggccgatgag
      601 agcctcggcg tgggtcgtct cgtgtttggc tgcgatcgcg atcggtgcgg cgttcgccca
      661 gccggcgccc gacacgcgct tcacggccga cggcgagcga cagattctcg aaatgagcga
      721 ctggctcgag aagcacccgc tcgccgcgga tcgctccgag gtgttcggca gggtgttgga
      781 atggtgggtc gaggccccgc agctcacgct caactggtgt ccggcgatcc tccgcgaggc
      841 ccagaacgac aagacgaact cgctcgtcac gacgcaggcg gtggtcggcg cgggagccca
      901 tctcatccgg catcgcgacg aggccgacgt cgtcgggatc ggcctggccg gggtcgagag
      961 cgcgctgcgc gcctacgaga gcgccgtcga gcaggatccg tcgtaccggg acccggcgta
     1021 cgaccgactg atcgagcgcc ggcaagccga cgggctgagc cggtacgtga ccgacaaact
     1081 caaggagtgc gagggagacg ccgagcgacc ctaccgcgac gtcacctgag cgggacgggc
     1141 cccgggcgtc aacccaaagt ggaaacccgt cttgttcatg aattgtccag tttttcaccc
     1201 aaacgagctg aatgctgaaa catagaaaaa cggccggagt ttctatccga cgcccaaaag
     1261 gccgtcgggt ggacccctaa agtcgtccac cgcatgtcgg cattggagat cgccttttcc
     1321 atcagcccgt tgtggctcgc gctcgctcgg ccgacggcgt tcaaggtgca cacggcgttc
     1381 ggtgagtaca cgcacctcta cctcgaccgc gacgagcgct tcctgtgcaa ctacacctcg
     1441 tggtgacggc gtcgcaagtc cactaccgcg cgctggccga ctcggtcctg gccctgcacg
     1501 ccgcgttcgt gatcttcgtc gtcgtcggcc tggcgctggt actggtcggc ggggcacggg
     1561 gctggcgctg ggtgcgcaac ccgtggttcc ggctggccca cctgctggcg atcggcgtgg
     1621 tcgtgctgca ggcctggctc ggcgtgctct gcccgctgac gacgctcgag atgcgcttgc
     1681 gcgagcgggc cggcgacgcg gtctaccgcg gcagcttcat cgcccattgg ctggaacggc
     1741 tgctctacta cgacgcgccg gcctgggtct tcgtcgtctg ttataccggt ttcgggctgc
     1801 tcgtggccgg gagctggtgg tgggtccgtc cgggaccgtt ccacgaccgg tctgcgctag
     1861 agtaggtcgg gaagggaagg gagatcgaga tgaaaccggt gatcaacttg gacgagctcg
     1921 agatcgaatc gcacgaccgc gggtcgttcg gcagccggta cggcgtgatc agcgatcgca
     1981 tcggcgcgcg caagctgggc tacaacctga cgatctgccc gccgggcaag cgggcctgcc
     2041 cgttccacaa ccaccatacc aacgaggaga tgttcctgat cctcgagggc gagggtctgt
     2101 tgcgcttcgg cgacgacgag tacccgctgc gaccgaacga cgtgatcgcc tgccctcccg
     2161 gtaatcgcga cgtggcccat cagatcatca acaccgggag taccgagctg aagtacctcg
     2221 cgctcagcac ccgcgagcgg acggaggtcg tcgagtaccc cgattcggac aagatcggcg
     2281 tgatggtcgg tccgttcggc gaccaagacc tgcgggcgat cttccgcgcc gaacagaccg
     2341 tcgactattt cgaccgggag gagtgacggc gggcgtggga cgcatcctgc ggatcgcacc
     2401 ggccgggcgg tgggacgccc gaagaccaga gagacgatcg atcaacggtg cgcggtcgat
     2461 tctgctcgcg gtcggtatgg cggccctcgc ggtcgggacg ccggccgacg agggccagcg
     2521 gttcgagaac gcgaccgccg gcatatcgct cgagcgtccc gccggctggg tcaccgcgtc
     2581 gatccagcag gtcgaggcca accgtaagcg ggtgcgcctt ccggatgacg agctgcaggc
     2641 cgcggtcgag gagcacgcgt cggccccgct gttcgtcttc acccgctacc ccgagcccca
     2701 cgacgacctc aacccgtcga tccaggtcat tctgcggccg ctgggcgagc ttgccggact
     2761 gccgccgatc gaggtgatgc aggccgcggt cggtccgttg caggagatct tcggcaactt
     2821 cgagttcgtc gaggagattc acccgaccga agtcgcgggt taccccgcgg cacgcatgtc
     2881 ggcgacctac acgatcgaga acgacgaggg acgagccttc gaaacggtgt cgaagatgtg
     2941 gatcgtgccg cgaggctcgt tcatgttcat gctcggcatg agcggtgcga tagaggaccg
     3001 cgacgagctg gaagagatct tcgaacgggc gatcggctcg gtcgagatcg cggattgacc
     3061 ggatgaccac gacgcggagc gcgaccgttg tcgcgacgat cgtgctcgcc acctgctgcg
     3121 ggtacgtcca gcgggggagc tggacggacg atccgcgcaa ctggtaccgc atcttcggcg
     3181 aggccaaacc ggacgaggtc acgttggtgc actcgttcta cgagcgtctg ccgggcttca
     3241 ggatgcgcta ccggttctac ctcgccttcg aagctccggc cgaggcgcgc gagcggctgg
     3301 tggcgcgcct ctcgctctcg ccgctgccgc acgatctcgc gccgaccgac gccgccggcg
     3361 cggatccggc ccggccggcg tggttcgcgc cgggcgaccc cggcgactac cggatctgga
     3421 tcgatgcccc gaaccgccgg ctgcggatgt tcgtaaaccc ggacacgggc gcgacctatc
     3481 tgagcgatcg ccgctcgtga gccggggggt tcgatggcgc cggttcgaac gacggggatg
     3541 ttgacgcggg cgaccttggc cgcggcgacc gacgcgctcg cggcgtgcga cgccgacctg
     3601 gcgcggatcg tcgagcgata cgggcggcca ccgctgtggt cgcgccggcc cggcttcgtc
     3661 acgctgctgc gcatcgtgct cgagcaacag gtctcgctgg cgtccgcgcg ggcgacctac
     3721 cggcggctgg ccgagcgcat cgacccgctg acgcctgaga ccgtgatcgc gggcggcagc
     3781 gtcgcgctgc gcgccgcggg gctgacccgg cagaagactg cgtactgtct ggacctggcc
     3841 gaggcgatcg cgcggggcgc gctcgacctc cgcgggctcg gccgcgtcga cgacgccacg
     3901 gtcagcgcgc ggctgacccg gatcaagggg atcggaccgt ggaccgcgga ctgttacctg
     3961 ctgatggcct tgcgccggcc ggacgtctgg ccggcgggcg acatcgcgtt ggcgtcggcg
     4021 gtgcgggagg tcaagggtct gcgccggcgg ccgaccccgg aaacgctgac gacgatcgcg
     4081 gaaggctggc gcccgcatcg ggccgcggcg gcgcggatgc tgtggcatta ctacctgagc
     4141 cggcgcgaac gaaagggagg caaggacgat gatcaagggc gtgcacacga tgttctacac
     4201 gtcggagccg gaggcgctgc gcgcattcct gcgcgacaag ttggggttcc cacacagcga
     4261 cgtcggtgac ggctggttga tcttcgacct gcccgaagcc gacatgggat gtcatcccgc
     4321 ggacgagggc agcgaggacg gcaagccgtc gggtacgtgc gacatctcgt tctactgcga
     4381 ggacgtcgtg caaacggtca aggagctcga ggcgcgtggc gtcgagttcg tcgacgaagt
     4441 cgagaaccac ggctacggcc tcgtgaccca tttcagcatg cccggcggca tcgtcgccca
     4501 gttgtaccaa ccgctgtacg acaagaggta gcggtggccg acgaacggca gccggacctc
     4561 gagcggctcg agcgaggcct ggtcgccatc ggccgatctc cgaccgacgc cggccgagtc
     4621 gagttgatcg cgtgccggcc gcgcgagggc gagcggcgcg tgctggaaca ggcgcgcctc
     4681 gacgtcggcg acggtttgat cggcgacgac tggaagaccc gtggcagccg ccgcaccacg
     4741 gacggtaacg cccatcccgg catgcagctc aacgtgatga actcgcgcgt gatcgggctt
     4801 ctcgccggag accgcgcgcg ctggccgttg gccggtgacc agctctacgt cgatctcgac
     4861 ctgagtaccg ccaacctgcc gcccggcacg cggctcgcgg tcggctcggc gctgatcgag
     4921 gtgacggccg agccgcatct cggctgtagg aagttcgagc tccggttcgg tctcgacgcg
     4981 ctgaagttcg tcaactcgcg ccagcgcaga cacctcaacc tgcgcgggat caacgcgaga
     5041 gtcgtgcgct cgggcgacgt gcgcctcggc gatgtcgtgc gcaagctgcc cgacctagcc
     5101 gacgcgcgcc gctagccgac gcgcgcgatc gcgtcgatct cgaccagtgc gccgaccggc
     5161 aaccgccgga cttcgacgca tgcccgcgcc gggtacggct cgctgaagaa gcgcgcgtag
     5221 acttcgttga ccggtttgaa gtcgtccatg ctcgtcaggt agacggtggt gcgcacgacg
     5281 cggtcgagac cggaaccccc ctcggccagg atccgtgcga gattctcgag cgcccgctcg
     5341 gcttgcgtcg ccgcgtcgct gccgacgagc ttcccgctcg ccgggtcgag tgccacctga
     5401 ccgctggtcc acaaccaacc gtccatgatc acgccctgcg agtacggacc gaccgccgcc
     5461 ggtgcgtgct tggtgctgac gatcctcttg ctcatcgctt ccccctgcgc ggacaccagc
     5521 atacatgctc cggccggccg ccggggcggc tataatcgcc ctgcgggatc gagagggccg
     5581 atgatgaacg aaggaaagcg gctgtacgcg gtgctttcgc tcgtcgcgct cgtcggtggt
     5641 gcggcggccg cgggccagta cgaggccaac gtacgtcgcg acgtcgcggc ctacttcgac
     5701 gcgttgcggg tctcggactt tccgacgttg gccggcctga cctgcccggc ggtcgtcgat
     5761 tcggtcggcg gctacggcgc cttcatcacc tcgctgcaag cgatggaccg ggaacgcgcg
     5821 gcgcagggta tcgagtgggg cgaggtgacg atcgaatccg tgtccgagcc cgtcgtcgcc
     5881 ggtgagcagc tgcacgtgct gatcgaggtt cgacagcaga tcggctcgcc gtcgggaaag
     5941 acgaagaaga aaacctacat gctcggcgtc tccgaggacg cgggtgacag ttggacgttc
     6001 gtcgacgcga ccaagctcga cgccgccaac aagcagaagc tcttccccga gttcaacgac
     6061 gcactgaagc taccgaaagg ctgagggagt cggaccgttg tcgtggcgtg actgggacgc
     6121 gttgtggggc gcactacccg tcatcgcgtg gatcctgatc atcgtgctcc gcggcctgaa
     6181 gacggaccgc gcgaggacag cacgaccggg gcgccccttg tcgacgcccg gcggcttcga
     6241 gcgggcgtac gagccgatcg agccgagctt ccgcggtcgc gtgccgacgc cgcccgtgcc
     6301 ggcgagcgag agtcggcgac gggccgtgcg cacgatcgac ctggggcagt ggatcgagga
     6361 cctcgcctcc gaccgaggcc cggacgacga cgagcgggca ccctcgttgc gaaccgaccg
     6421 ggacggctga gccgatcagg cgagggactc gatcagcgcg gcgaccgcgc cggccgggtc
     6481 gcggatcacg cagtagcggc cgtgcgagcc catcgacttc ggtccgttga cgaccgtgcc
     6541 acccagctcc tcgcaggcgg cgatgctgcg atcgaggttc tcgaccgtga tgtagaccag
     6601 ccactgcgcc ggtagcccct cgttcgtccc gcgggcgtgg cacacccccg cgacggcgtc
     6661 gccgctcgcc ggtgcggtca tgctgaagtc gctgtagccg cccatctcga ccggggccac
     6721 gccccaaccg acgacgcggc gatagaaatc acgcagtcgt tcggcatcgt cgaccgtcaa
     6781 gtcggtccag acgaccgtgc cgaccggcgg tttgttcccg tcgcccatcg cccctcctcg
     6841 actgaggtcg gcagtatagg ccgaatcacg gcgcgaaccg cattcgatcg ccgatcggac
     6901 tagaatgggg aatcctcagg gggtgggtca tgagcgaaca gacggcggcg atcccacgcg
     6961 aggcgagaaa ctgggcgatg ggcgcgcaca tgatcgcgct cgtcggatta ctcggcaacg
     7021 gtctgggttt tctcgtcggg ccgttgatcc tgtggctgct caagcgtgac gcgcacccgt
     7081 tcatcaacga acagggcaag gaagcggtca acttccagat cacgatgctc ggggcgatcc
     7141 tggtctgcat cccgttgctg ttcgtcggga tcggcatctt gctgatgccg ctgatcgggc
     7201 tgtacgacgt ggtgatgacg atcatcgccg gcatcaaggc cgccgacggc acgcactggc
     7261 gctacccgct gacgatgcgc ctgatcgcct gacctccggg ccgggattgt gacagtcgac
     7321 catgccgtgg cgcgatttgc gccctttggc ggtagaatct ggcccgggtc gagcacggtc
     7381 cgcggagcca gacagcgtga ggaagatcct gtattgggtc ggcggcgtcg ctgtggcggc
     7441 gttcgtcgcg atccagttcg tgccggtcga tcgggcgaac ccgccggtcg aggccgacct
     7501 ggtcgccccc gacgacgtcc ggcaggtgtt gcgcgtggcc tgttacgact gccactcgtt
     7561 cgagacgcgg tggccgtggt acagccacat cgcgcccgtt tcgtggctcg tggctcgtga
     7621 cgtgaggtcg gcgcgcgcgg agctggtctt ctccgaatgg gagcggatga gcgcccggga
     7681 gcaggccgag ctgcgcgagg agatttggga ggaggtcgaa gagggcgaga tgccgctcaa
     7741 ggcctatcgc ctgatgcacg ccgaggcgcg tctcgggccg gaccagctcg agacgctcag
     7801 acgctgggcg catcagtccc agccaaacta gtaaggggag ggaaccgcga atgggtaaga
     7861 aagagaagga caagaaaaag aagaaggaca agaagaaaaa gaaggcgaag gacaagctgg
     7921 tcgagcagtc gcttgcggcc gatcgcgcga cggtcgagga gtcggagacg atcctcgagc
     7981 agattgggcc gagtttcctc gaacacggcg cgcacctcgt gcgcgcaacc gacctggctc
     8041 gcgtcgtcga ccagcgagac gagctgaccg agcgattcgg cgccgatcgc ccactcggcc
     8101 ggttccgcga cgaggcgcgg ttgatgctgt cgttgctgca ggatttcaga gcaggccagt
     8161 acaccgacat ctcgtattgg agcatcgcgg tgatggtctt cggcttccag tacgtgctga
     8221 agccgatcga catcatcccg gacagcctgc cggcgatcgg tcagctcgac gacacggtgg
     8281 tgatgtccca ctgcctcgag atgattcaac ccgagctgtt ggactacaag gtgcggatgc
     8341 tgacacgcga ggagagctga ccttcgaatc gcagctacga gcgcggcgcg agggatttga
     8401 acaaggcggt cgctgcgccg tacgacgtga ggcgcagcgt cagctttgta aacaggtggg
     8461 cttgtccggc gaggaaggcc agtgcgattg ccggaacgcc ggactggccg ggcccgggcg
     8521 ccagccacga gtagaccacg agcagcagca gcgagaccgc agccaagccg aactgcaggc
     8581 cgagggtctt cgccgggaag ccggcgacga agcgcagtcc gcgcagcgcc gacgggaaga
     8641 tcgaggcgcg gttctcgacg accgtcgcga tcttggcgta ggcgaaggtc atctgcacca
     8701 gcgtcagcag cagcgcggtc agcagataga gggcgagcgt gagccacagc acggtccact
     8761 cgcgggtggc gtcccgggtc aggtactcga gccaaccgag cagcaggcga tgcaggagat
     8821 agatgccgag atacagcacc cccgacagca aggccagcct gaggaaacga aagaagtaga
     8881 tcccgccggc ctgcgcgaac cggcctcgca cggccgattc cgccggccgc gcgaagcgat
     8941 cgagcacgcc gccgagcaag aagacccaga gcacggccca tgccgcgccg gccgcgacca
     9001 cctccgggta ctcgacgaac aacgtgcccg cgacccagtc ctcgagattg tcgtagaacg
     9061 ccgtcgcacc gatcaccgtc ggacgaaagc tggcggccag accgtccgcc cggtgggcga
     9121 actcgccgta ccagccgccg tcgaacccgc gccgcagctc ctcgtggacg cggctggcac
     9181 cgatcgactc gccgatcgcg cggccgacga gagcggcgaa cggcagggcg aacagcaggc
     9241 tcaccagcca cagaccgacg aggatgcgcg gcgcggccaa cgtcgcgccg aaccctctca
     9301 tcgccgcgcc gaagatcgac acgcccgtct cctagatgaa gtacgcgaag gtggccatcg
     9361 cgtcctggaa ccagatcatc cacttggagg cccacttgac ggcgggcagt ctcgcacggg
     9421 gctcgcgcaa gcggctgttg ttcgtgtagt cgacatcgag catcagcacg cggtctgggt
     9481 cgacctcggc ccgctcgatc ttggccgcgc gctcgacgac gaacaacttc gagcgctcct
     9541 cgccggacca gtgcttgcgg atgcgctggc cgtcctcgaa ggtcagcacc acgtcgaccg
     9601 gaaagacccc gccaccgacc cgccggacca tcacctcggt acggaaatcg ctgccttggc
     9661 cctcgcggcg cgtccgcacc atctcaccgt cgcgttgcgt gaagccctcc gcctcgaccg
     9721 gcgcggagtg gaccgaatcg accgcgtagt cgaagacgac cgcgtcgcgg tagacctgat
     9781 cgaagaacca gtccatcggc ccggcgaact cctcgaccgt gtcgagaaag gcgtcgggcg
     9841 tcgcgtggcc gaagcggtgg cgctcgaaga aggtcgagag aatcgtctgc agcgtttccc
     9901 agccgaggtg acgctcgagc gtcgcgagcc agagcgcggt cttgtcgtag gacaacgacg
     9961 aaccgccgtc cgggtagtac tcgaaggtga agccgtcgag atcggcgact tcggcatgct
    10021 cgcgatagcg atcgaggcgc cgcgtgaagc gcgacagctc gaccccgtcg atcgacaccg
    10081 gcaggaaccc gcgcgaggaa tcctcgccgg gcggcgcgag gtagcgctgg accagctcga
    10141 gcggcgggta ggtcgcgtcg agcgtgcgga tcgtcgagaa cgtgttcaat ccttcgtcga
    10201 tccaggcatg ctcgaactcg ttgttggcga cgacgccgta ccagaactgg tgtcccacct
    10261 cgtggatcgt caccgattcg gggctgtcgg cgccgaacgg attgaagatg cgcgtgccgc
    10321 aagtgaagaa ggtcgggtat tccatcccgc cggtgtcgct gccgtaggcc gggtcgacca
    10381 cggtgacgtg gccgtaggga tagggcccgt accacgtgcc gtagtgctcg agcccggcgg
    10441 ccgtcgcgtg aaagtgacgc tccgcctgcg ccagatgctc gggctgcagc agcagccgta
    10501 tgtcgaccgg tggaagaccg gcgcgttcga atcgttgctc gcgcacgacg aagtcggggc
    10561 tcgtcgtcca actgaaggcg tggacgtcgt gctgctcgta gacgtgagtc tcgctgccgt
    10621 cgtcgtgcgc cgtgcgctcg acctcgcgtc cggtggcgcc cagcacccac ccggccggga
    10681 ccgtcatcga cacgcggtag ttgccgaagt ccgaatagta ttcggtcaga ttgtggtact
    10741 ggtggcagtt ccagccgtcc tcctcgaaca cccccaactt ggggaaccag tgggcgaaaa
    10801 agaaatagtc gccgcggaat cccgtgcggg cgaaggtacg cggcacgcgt gcgcgccacg
    10861 ccaactcgac ctgcacctcg ccgccgggct cgaccgcgaa cggcagcggt acgacgaaca
    10921 ccgtacgatc ctgcgggttg ccgtcgtccg gcgcggcgta gtacgaggcg gtcagcagct
    10981 cgagcggtgc ctgctcgtgg ccgggtc
//
LOCUS       DASPGN010000588         7126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1281482, whole genome shotgun sequence.
ACCESSION   DASPGN010000588 DASPGN010000000
VERSION     DASPGN010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7126
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281482"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VD788_17025"
     CDS             complement(<1..325)
                     /locus_tag="VD788_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78015.1"
                     /translation="MSHPEAGRLERFDQLGPTERREIAVHVASCSACRLRLLAAEPTH
                     LFSLLGDGGIPAGALDRLAVSLGDEIDRQDRRGRRVGPLWGSIAASLLLGALIGVYLS
                     SRPAGT"
     gene            complement(322..939)
                     /locus_tag="VD788_17030"
     CDS             complement(322..939)
                     /locus_tag="VD788_17030"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV78016.1"
                     /translation="MPPSGDEQALIRAAAAGDRRAFDELVRLKRERVVRTAYQITGDL
                     DDALDVAQGVFLKLWRGLERFDPRRRFDTWVYRITVNAAIDMLRSRGARGTVQPLPDE
                     IASGEPTPETDPARELDRREVQRAFLRLAARLAPRQRAVFVLREIEGLGTSEIAAAMG
                     VTESTVRNHLLQARRTLREGLEREYPGLVPRGSVASDQASGEDER"
     gene            complement(1072..3492)
                     /gene="lon"
                     /locus_tag="VD788_17035"
     CDS             complement(1072..3492)
                     /gene="lon"
                     /locus_tag="VD788_17035"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXV78017.1"
                     /translation="MSLSSVRAEEEPLSIPQELPVLPLRDLVVYPFIISPLSVARGLS
                     IQAVDQSLSENRMILLATQRDKDDEDPDGDGLFRVGTVAVIMRMLKLPDGRIRVLVQG
                     ICRARIVSFSGERPFFSARVERIEDQPHDPDSIEKEALMRSIKRLLERASSLGKNLPS
                     EVMVIANNLEDPGRLADLCSSNLELKVEEAQEILETLDPLARLKRVHDYLKRELDLLA
                     MQREIDSLARDEMDRSQREYYLRHQMKAIMTELGEGNELAEEVEQYRAKASGLKISAE
                     GREELDRQLKKLERMHPDSSEATTVRNYLDWLTSLPWGRLTRDNLDLAKGAQILDDDH
                     YGLERIKDRILEYLAVRKLKKQSKGPILCFVGPPGVGKTSLGRSIARALGRKFVRLAL
                     GGVKDEAEIRGHRRTYVGAMPGRIIQGLHQAGSNNPVFMLDEVDKIGADHRGDPSSAL
                     LEVLDPEQNNAFRDHYLGIPYDLSDVMFITTANLLDPIQPAFRDRMEVIPLSGYTEEE
                     KLIIAARYLVPRQIERNGLREEQIDFSQNALKTLIREYTREAGLRNLERTVAALCRKV
                     ARQVAEGKRERIRIRSSSIEGFLGASPLTREKALSEDQIGVAIGLAWTESGGDVLFVE
                     ATTMEGRGQLLLTGQLGDVMKESAQAALSYARARAADLGIPRGYFEGRDVHIHVPEGA
                     IPKDGPSAGITMAATMLSAFTQRPIRGRVAMTGEITLRGNVLAVGGIKEKVLAARRSG
                     IREVILPEANRKSLEEIPRSLRRDLRICFVGHVQEVFDAVLIDPSAKRGKTSPRTSPA
                     AVLQPTPG"
     gene            complement(3489..3929)
                     /locus_tag="VD788_17040"
     CDS             complement(3489..3929)
                     /locus_tag="VD788_17040"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXV78018.1"
                     /translation="MPSPLGPLMEVARIQSEINRLFDNLLDLGKTEEVGSWNPNVDIV
                     ETETELLLKVELPGVRAELLELSVHGGNLILRGEKERPRLAGRAEFQLAERAFGAFRR
                     VIHLGVAVNTHHAKAVLNNGLLRISFPKVPNRRGEAVPIEVHSE"
     gene            complement(3947..4405)
                     /gene="nrdR"
                     /locus_tag="VD788_17045"
     CDS             complement(3947..4405)
                     /gene="nrdR"
                     /locus_tag="VD788_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXV78019.1"
                     /translation="MKCPYCSSTKDKVVDSRESGSGDAIRRRRECQGCGRRFTSYERI
                     EEIPYLVIKKDGRREAFDRAKLMAGLHRACEKRPVSAKELERLGDDIEQLVQDAVDRE
                     VETRVIGECVMERLKELDKVAYVRFASVYRQFEDVAAFMAELKDLLETRK"
     gene            complement(4402..5451)
                     /locus_tag="VD788_17050"
     CDS             complement(4402..5451)
                     /locus_tag="VD788_17050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXV78020.1"
                     /translation="MRDLAKYAAGIVLAVALLAWVVRDADLGAVWQQLREASLPLIAL
                     CVVLNTGHNVFRAWRWRALLAPFRTAVPFRPMFVAIIVGYMTSWIVPGRLGELVRPML
                     LSARENVPLGPSLGSVVADRMMDALAVVALFAVGAWATPLEGRAAEYAPLIRTGALTI
                     AGVVVGVVVAMMLASRSSGRLDRWLDGKPRLLRWAGRSLVAVASGVEALRSPRLLAVV
                     SIQSLFAWVTIALATWCGILASGVDIGLGPVLVITPLLVLGVAVPTPGGAGSYHGAMK
                     VGLMLFGVPDVQAISAGFLMHLVIVVPTVVLGMLLIWVEGLSLRDLVASASQLRRLGS
                     VPDAGRTQRALENAP"
     gene            complement(5515..>7126)
                     /locus_tag="VD788_17055"
     CDS             complement(5515..>7126)
                     /locus_tag="VD788_17055"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HXV78021.1"
                     /translation="SVVVSLQAELPWIGAAAGRWEARRKRATPFANALRAALRGGVLV
                     RVRKTGPDRIVRLEWGDGHVVVAELATHGANLILLDAGGRVTAVARHPRSSQRRLAPG
                     AVYLPPAIPARLFVPFGRTPEEIDAAIAAIGDREPELLERLRRQMFGVGTDGARLVVD
                     EATSSGRSVGEVLARRLAAIERGEADPVIVPDEDPLDAALAGTLDEARLRLLPWAAEG
                     SHGLRRNDAAATAGLYHEAVEQARRLRARAAGLRSIVERELRRLSSAERRAALDHAGF
                     GDPDTHRRWGEALLAGLSTARRHGEVALVADPYDPTARDLAVPVAPGVPLAEAAAGHF
                     QASRRARRGLDQARRRLDQLAARRVRLEGLIDRGDGLGGVDAIRSFEAAMLAERIPVG
                     LEPDRRSRATARLDRPRLEGVRVFETTDGSTILVGKGGRENHRLTFRLSAPEDFWLHV
                     EDAPGAHVILRNPERAPEPPPRALDEAAAIAAWYSDLRGDERVTVRWTQRKNVRKPRG
                     ATPGTVTLKRFRTVRARPRLPDEASAAR"
BASE COUNT         1081 a         2564 c         2419 g         1062 t
ORIGIN      
        1 cggttcccgc cggccgcgac gacagataga cgccgatcag cgcgccgagc aggagcgatg
       61 ccgcgatcga gccccacagc ggccccacgc ggcgcccacg tcggtcttgc cgatcgatct
      121 cgtcgccgag cgagaccgcc aaccggtcga gcgctccggc cgggatcccg ccgtcgccga
      181 gcagcgagaa caggtgggtc ggctcggcgg cgagcagacg caggcggcac gcgctgcacg
      241 atgcgacgtg caccgcgatc tcccgccgtt ccgtcggacc gagctggtcg aatcgctcca
      301 gccggccggc ctccgggtgg ctcaccgctc gtcctcgccg ctcgcctggt cgctcgcgac
      361 ggagccgcgc gggacgagac cggggtattc ccgctcgagg ccctcgcgaa gcgtacgtcg
      421 cgcctggagc agatggttgc gcacggtcga ttcggtgacg cccatcgccg ccgcgatctc
      481 gctcgtgccg agaccttcga tctcccgcag cacgaagacc gcgcgctgtc gtggggcgag
      541 acgcgccgcc aaccgcagga aggcgcgctg cacctcgcgg cgatcgagct cgcgcgcggg
      601 atcggtctcc ggggtcggtt cgccggacgc gatttcgtcg ggcagcggct ggacggtgcc
      661 ccgcgcccca cggcttcgca acatgtcgat ggcggcgttc acggtgatcc gatagaccca
      721 cgtatcgaag cggcggcgcg ggtcgaaccg ttcgagaccg cgccacagct tcaggaagac
      781 gccctgggcc acgtccaagg cgtcgtccag gtcgccggtg atctggtacg cggttctgac
      841 gacgcgctcg cgcttgagcc ggaccagctc gtcgaaagca cgccgatccc ccgcggcggc
      901 cgctcggatc agcgcttgct cgtcaccaga tggcggcatc cgcaggacgg actcccccgc
      961 aggaagaccg ccggcacgct cttgttacgg aacgcgggac cccggcgtct aggctcctgc
     1021 gcgaaatcgg tcaacaccga caggagccgc ttactttagc agccggcggg gctatccggg
     1081 ggtcggctgg agcaccgccg cgggactggt gcgcggggag gtcttacccc gtttggcgga
     1141 cggatcgatc aacaccgcgt cgaacacctc ttggacgtgg ccgacgaagc agatccgcag
     1201 gtcgcgacgc agcgaacgcg gaatctcctc gaggctcttg cggttcgcct cgggcaggat
     1261 cacctcgcgg atgcccgacc ggcgcgcggc cagcaccttc tctttgatcc cgccgacggc
     1321 cagcacgttg ccgcgcagcg tgatctcgcc ggtcatcgcc acccgcccgc ggatcggccg
     1381 ctgggtgaag gccgacagca tcgtggcggc catcgtgatg ccggccgacg gaccgtcctt
     1441 gggaatcgcg ccctccggca cgtggatgtg cacgtcgcgg ccctcgaagt agccgcgcgg
     1501 gatgccgagg tcggccgcac gcgcgcgggc gtaggacagc gcggcctggg ccgactcctt
     1561 catcacgtcg cccagctgac cggtcaagag caactggccg cggccctcca tcgtcgtcgc
     1621 ctcgacgaac aacacgtcgc cgccggactc ggtccaggcc agaccgatgg cgacgccgat
     1681 ctggtcctcc gacagcgcct tctcgcgcgt cagcggcgaa gcgccgagga agccttcgat
     1741 gctcgacgac cggatgcgaa tccgctcgcg cttgccttcg gcgacctggc gcgccacctt
     1801 gcggcagagc gccgcgacgg tgcgctcgag gttgcgcaga ccggcctcgc gggtgtattc
     1861 gcggatcagc gtcttgagcg cgttctgcga aaagtcgatc tgctcctcgc gcagcccgtt
     1921 gcgctcgatc tgccgcggga cgaggtagcg cgccgcgatg atcagcttct cctcctcggt
     1981 atagcccgac agcgggatca cctccatccg gtcgcggaac gccggttgga tcggatcgag
     2041 caggttcgcc gtcgtgatga acatcacgtc cgacagatcg tacgggatgc cgaggtagtg
     2101 gtcgcggaag gcgttgttct gctccgggtc caacacctcg agcagcgccg aggagggatc
     2161 gccgcgatga tcggcgccga tcttgtcgac ctcgtcgagc atgaacaccg ggttgttcga
     2221 gccggcctgg tgcagaccct ggatgatgcg acccggcatc gcgccgacgt aggtccgccg
     2281 gtgtccccgg atctcggcct cgtccttgac cccgccgagg gccagccgca cgaacttgcg
     2341 tccgagggcg cgggcgatcg agcgaccgag cgatgtcttg ccgacgccgg gcggcccgac
     2401 gaagcacagg atcgggccct tgctctgctt cttgagcttg cgcacggcca ggtactcgag
     2461 gatgcggtct ttgatgcgct cgaggccgta atggtcgtcg tcgaggatct gtgccccctt
     2521 ggccaggtcg agattgtccc gggtcaggcg gccccacggc aggctcgtca gccagtcgag
     2581 gtagttgcgc acggtcgtcg cctcggacga gtcggggtgc atccgctcga gcttcttcag
     2641 ctgtcgatcg agctcttccc gcccctcggc gctgatcttc aggccgctgg ccttggcgcg
     2701 atactgctcg acctcctcgg ccagctcgtt cccttcgccg agctcggtca tgatcgcctt
     2761 catctggtgc cttaggtagt actcgcgctg cgatcgatcc atctcgtcgc gggccagcga
     2821 atcgatctcg cgttgcatcg ccaacaggtc cagctcgcgc ttgaggtagt cgtggacccg
     2881 cttcagccgc gccagcgggt cgagcgtctc gaggatctcc tgggcctcct cgaccttcag
     2941 ctcgaggttc gacgagcaga ggtcggcgag gcggcccggg tcctccaagt tgttggcgat
     3001 caccatcact tcggacggca ggttcttgcc gaggctcgac gcccgttcga gcaggcgctt
     3061 gatcgatcgc atcagcgctt ctttttcgat cgagtcgggg tcgtgcggtt ggtcttcgat
     3121 ccgctcgact cgcgcgctga agaacggacg ctcgccgctg aagctcacga tacgggcgcg
     3181 gcagatgccc tgcaccagca cgcggatccg gccgtccggc agcttcagca tgcgcatgat
     3241 cacggccacc gtcccgacgc gaaacaggcc gtcgccgtcg ggatcctcgt cgtccttgtc
     3301 gcgctgcgtc gcgagcagga tcatccggtt ctcggacagc gactggtcga ccgcctggat
     3361 cgacagaccc cgagcgaccg acaacggcga gatgatgaac gggtagacca ccaggtcgcg
     3421 caacggcagc accggcagct cttgcggaat cgacagcggt tcttcttcgg cgcgcacgga
     3481 cgacaggctc attcgctgtg gacctcgatc ggtacggctt ctccccgacg gttcggcacc
     3541 ttgggaaaac ttatgcgcag cagtccgtta ttcaagaccg ctttcgcgtg gtgggtgttg
     3601 acggccacgc cgaggtggat cacacgacgg aaggcgccga acgcccgctc ggcgagctgg
     3661 aattcggctc tgccggcgag ccgcggccgc tctttctcgc cgcgcaggat caagttgccc
     3721 ccgtgcaccg acagctcgag cagctcggct cgaacgccgg ggagctcgac cttgagcaac
     3781 agctcggttt cggtctcgac gatgtcgacg ttggggttcc agctgccgac ttcctcggtc
     3841 ttgccgaggt cgagcaggtt gtcgaacaac cggttgatct cgctctggat gcgggcgact
     3901 tccatcagcg gtcccagcgg gctcggcatc gcgtctcctt ctgccgctac ttgcgggttt
     3961 ccagcagatc cttcagctcg gccatgaacg ccgcgacgtc ctcgaactgc cggtagacgg
     4021 aggcgaagcg aacataggcg accttgtcca gctccttcag ccgctccatc acacactcac
     4081 cgatgacgcg cgtttcgacc tcgcggtcga cggcgtcctg caccagctgt tcgatgtcgt
     4141 cccccaggcg ctcgagctcc ttggccgaga ccggccgctt ttcgcaggcg cggtgcagcc
     4201 cggccatcag cttggcccgg tcgaacgcct cgcggcgccc gtctttcttg atcaccaggt
     4261 agggaatctc ctcgatccgc tcgtaggagg tgaagcgccg gccgcagccc tggcactcgc
     4321 ggcggcggcg aatcgcgtcg cccgacccgc tctcgcgcga gtcgaccacc ttgtccttcg
     4381 tggagctgca gtacggacac ttcacggtgc gttctccaga gcgcgctggg tgcgacccgc
     4441 atccgggacg gagccgagcc ggcgcagctg gctcgccgac gccaccagat cccggagcga
     4501 cagaccctcg acccagatca gtagcatacc gaggacgacc gtcggcacga cgatcaccag
     4561 gtgcatcagg aaaccggcac tgatcgcctg cacgtccggc acgccgaaca gcatcaggcc
     4621 gaccttcatc gcgccgtggt aggaccccgc gccgccgggc gtcggcacgg ccacgccgag
     4681 caccaacagc ggcgtgatga cgagcaccgg gccgagcccg atgtcgaccc ccgacgccag
     4741 gatgccgcac cacgtggcga gtgcgatcgt gacccacgcg aacaggctct ggatcgacac
     4801 cacggcgagc agacggggcg agcgcagcgc ctcgacgccg ctcgccaccg cgacgagcga
     4861 ccggccggcc cagcggagca ggcgcggttt gccgtcgagc caacgatcga ggcgacccga
     4921 ggaacgggaa gccagcatca tcgcgacgac tacccccacg accacgccgg cgatggtcaa
     4981 cgcgccggtg cgaatcaacg gagcgtactc ggcggcccgg ccctcgagtg gcgtcgccca
     5041 cgcgccgacg gcgaacagcg cgaccacggc cagcgcgtcc atcatccggt cggcgacgac
     5101 cgaaccgagc gacggcccga gcggcacgtt ctcgcgggcg ctcagcagca tcgggcgcac
     5161 cagttctccc aaccgtccgg gcacgatcca cgacgtcatg tagccgacga tgatcgcgac
     5221 gaacatcggg cggaacggca ctgccgtccg gaacggcgcg agcagcgccc gccagcgcca
     5281 ggcgcgaaaa acgttgtgac ccgtgttgag cacgacacag agggcgatca gcggtagcga
     5341 cgcctcgcgc agctgctgcc agaccgcgcc gaggtcggcg tcgcgcacga cccaggcgag
     5401 gagcgccacg gcgaggacga ttcccgcggc gtatttggcc agatcccgca caccgtgagc
     5461 cccccgggaa ccccctcggc tccgcgccga atctaggcca gcgacggcca aacgtcaacg
     5521 cgcggccgac gcctcgtcgg gcagccgtgg ccgggcacgg accgtacgga atcgtttcag
     5581 cgtgacggta cccggcgtgg cgccgcgggg cttgcgaacg ttcttccgct gcgtccaccg
     5641 caccgtgacc cgctcgtcgc cgcggaggtc gctgtaccac gcggcgatcg ccgccgcttc
     5701 gtccaacgcg cgcggtggag gttcgggtgc gcgctcgggg tttcgcagga tgacgtgggc
     5761 gcccggggcg tcctcgacgt ggagccagaa atcctcgggc gccgacagcc ggaaggtcaa
     5821 gcggtggttc tcccggccgc cctttccgac caggatcgtc gacccgtcgg tcgtctcgaa
     5881 cacgcgtact ccctcgagcc gcgggcggtc gagccgtgcc gtcgcacggg aacggcggtc
     5941 gggctcgagc cccaccggga tccgctcggc aagcatcgcg gcctcgaacg accggatcgc
     6001 gtcgacgccg ccgagcccgt ccccgcggtc gatcaggccc tcgagccgca cccgccgggc
     6061 ggcgagctgg tcgaggcgcc ggcgagcctg gtcgagcccg cgacgcgcgc ggcggctggc
     6121 ctggaaatgg ccggcagcgg cctcggcgag cggcacgccg ggtgcgaccg ggacggcgag
     6181 atcgcgggct gtgggatcgt aggggtcggc gacgagtgca acctctccgt gtcgccgagc
     6241 cgtcgaaaga cccgcgagca gcgcctcgcc ccagcgacgg tgggtgtcgg gatcgccgaa
     6301 gccggcgtgg tcgagcgcgg cccgtcgctc ggccgagctc aggcgcctca gctcgcgctc
     6361 gacgatcgag cgcagaccgg ccgcacgggc acgcagtcgc cgggcctgct cgaccgcctc
     6421 gtgatacagc ccggccgtcg cggcggcgtc gttccgtctc agcccgtggc tcccttccgc
     6481 cgcccagggc agcagccgga gccgcgcttc gtccaacgtc cccgcgaggg cggcgtcgag
     6541 cgggtcctcg tcgggcacga tcaccggatc ggcctcgccc cgctcgatcg cggccagtcg
     6601 tcgggccagc acctcgccga cggaccggcc gctcgacgtc gcctcgtcca ccacgagccg
     6661 tgccccgtcc gtgcctacgc cgaacatctg ccgtctgagg cgctcgagga gctcgggctc
     6721 gcggtcgccg atcgccgcga tcgcggcgtc gatctcctcc ggcgtccggc cgaacggcac
     6781 gaacagccgc gccgggatcg ccggcggcag atagaccgcg cccggcgcca gacgacgctg
     6841 ggacgagcgc ggatggcggg cgacggcggt gaccctcccg ccggcgtcga gcaggatcag
     6901 gttggcgccg tgggtcgcga gctcggcgac gacgacgtgg ccgtcgcccc actcgagacg
     6961 cacgatccga tcgggtccgg tcttgcggac acgcacgagc acgccaccgc gcagggcggc
     7021 gcgcagcgcg ttggcgaacg gcgtcgcgcg cttgcgacgg gcctcccagc gtccggcggc
     7081 cgccccgatc cacggtagct cggcttgcag cgaaaccacg accgat
//
LOCUS       DASPGN010000589         5322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_346244, whole genome shotgun sequence.
ACCESSION   DASPGN010000589 DASPGN010000000
VERSION     DASPGN010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346244"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VD788_17060"
     CDS             complement(<1..678)
                     /locus_tag="VD788_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78022.1"
                     /translation="MTDDELLDRLSELCGILREYRDIWGRPRPVERDVVRALLAAMGL
                     RVETPAEIRDEIRARDEAPWRRALPPVLVARAGGPIVVPVGSPEAKLGERLRVVLETE
                     DSRRVEQGFIPRDCAETGRRSIDGTAYSRRALALEARPALGYHRLTLLAGDGSALGSM
                     RLIVAPDRCFVPAALEDAGRVWGPAVQLYAVRSGRDWGAGDFGDLDRLVRACGSLGAG
                     IVGLNPLH"
     gene            complement(675..2531)
                     /gene="treZ"
                     /locus_tag="VD788_17065"
     CDS             complement(675..2531)
                     /gene="treZ"
                     /locus_tag="VD788_17065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HXV78023.1"
                     /translation="MADAVVRRAAAMPFGAEVGPHGVRFRLWAPGAARVDLVLGDPRH
                     GVEERETDRLADGFWQLEHPAGAGTLYGYRVDGGPPVPDPASRYQPRDVHGPSEVIDP
                     RSFAWNDAGWSGRPWEETVLYELHAGAFSRQGGYDGVRRGLDRLAALGVTAIELMPLS
                     DFPGTRNWGYDGVLPYAPDSSYGRPDELKRLVAAAHRRGLMVFLDVVYNHFGPEGNYL
                     HRYARPFFHPDATTPWGDAIRFDGPDSATVRRFFIHNALYWLEEYHLDGLRVDAVHAI
                     HDESSPSFLDELAARVQAGPGARRHVHLVLENVANEARWLRRAADDRTPRCYVAQWND
                     DVHHALHCLLTGERGGYYADFVVDPARRLARALAEGFAWQGEHSSYRGGPRGEASAHL
                     PPTAFVGFLQNHDQVGNRAFGERLTVLAGERSLAAAVAVLLLAPAPPLLFMGEEYGCR
                     RPFPFFCDFGPELARAVTEGRRRGLARFVEFAGAADRGRIPDPNLRATFESAVLDDDE
                     RETAVARRWLAHYRTLLELRRREIVPRLAGCAGHSGRWNLAGERTPRVEWELGDGAKL
                     TLIAHLDDGDGPRVELPRGRLLWPATGPLAAGAETHRLDAWEVLWLLDEARR"
     gene            complement(2524..4425)
                     /gene="glgB"
                     /locus_tag="VD788_17070"
     CDS             complement(2524..4425)
                     /gene="glgB"
                     /locus_tag="VD788_17070"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXV78024.1"
                     /translation="MSELTDYDLYLFGEGKLWRIWDKFGAHVVERDGRRGTRFAVWAP
                     NAEWVAVVGDFNGWDETHDRLEPRGSSGVWEGFLPAVDHGALYKYAIRSRHGGYRVEK
                     SDPFAFACETRPATASVVWDLSGHSWGDADWMETRRSRNALDAPIAIYEVHLGSWMRA
                     DGDRGRWLSYGELAPKLADYCHRLGFTHVELLPVSEHPFDGSWGYQTIGYYAATSRFG
                     TPQELMALIDTLHRNGIGVILDWVPAHFPKDDHGLGYFDGTHLYEHADPRQGQHPDWG
                     TLIFNYGRNEVRNFLIANALFWLELFHVDGLRVDAVASMLYLDYSRNEGEWIPNKYGG
                     RENLDAIGFLKQFNTTVYQQHPDVITIAEESTAWPMVSRPTYLGGLGFGLKWNMGWMH
                     DTLQYIAKDPIHRNFHHHQMTFGMMYAFHENFVLPLSHDEVVHGKGSLIGKMPGDPWQ
                     RFANLRTLYGWMWMHPGKVMLFMGGEFAQWREWDHDRGLDWPLLEQAPHRGLQRWVRD
                     LNTLVRGEPALHQRDFQSDGFQWIDCNDSAQSTLSFLRWDAERKRPIVCAFNFTPVPR
                     SNFRLGVPRAGGWHEALNSDATIYGGSGQGNLGAVETAPVAAHGHFQSINVTLPPLAA
                     LVFRTAAGG"
     gene            complement(4437..>5322)
                     /locus_tag="VD788_17075"
     CDS             complement(4437..>5322)
                     /locus_tag="VD788_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78025.1"
                     /translation="RLFADYLERALATGGREAVLPDAGTPLPALARRRPPVVVERCAA
                     SSLAAARGLGRRTAEFHLALASDRRDGAFAPIPMTTDYLRALADDCARRAEQVVESLN
                     ANLAALPASVASQAHELLSDSSALIGRFRGLDRLRTDAWRIRVHGDYHLGQVLHVAEN
                     DFVLLDFEGEPLRTIGERREKYSPLKDVAGMIRSFGYAAHSAFIEAVGRHPERIAVRE
                     PWVRAWETWVAAAFLGAYLESAGDSPIVPGEADALQALLEAFLLDKAIYELGYELNHR
                     PDWLPIPFEAIRSIAVKP"
BASE COUNT          818 a         1909 c         1858 g          737 t
ORIGIN      
        1 gtgcagcggg ttcaagccga cgatgccggc gccgagcgag ccgcaggcgc gcaccagccg
       61 gtcgagatcg ccgaagtcgc cggcgcccca gtcgcggccc gagcgcacgg cataaagctg
      121 gaccgccggt ccccacacgc gtcccgcgtc ctcgagcgcg gcgggcacga agcaacgatc
      181 gggcgcgacg atcagccgca tcgaaccgag ggcggaaccg tcgccggcga gcagcgtcaa
      241 ccggtggtag ccgagcgccg gtcgcgcctc gagcgcaagg gcgcgacggc tgtacgcagt
      301 cccgtcgatc gaacggcgcc ccgtctccgc gcagtcgcgc gggatgaagc cttgctcgac
      361 ccgtcgcgag tcttcggttt cgagcacgac gcgcaaccgc tcgccgagct tcgcttcggg
      421 cgacccgacc ggcacaacga tcggacctcc ggcccgcgcc accagcaccg gcggcagcgc
      481 gcgacgccac ggcgcctcgt cccgtgcgcg gatctcgtcg cggatctcgg ccggcgtttc
      541 gactctcagg cccatcgccg cgagcagcgc acggaccacg tcgcgttcga ccgggcgcgg
      601 ccgcccccag atgtcgcggt actcccgcag gatgccgcac agctcggaca gccggtcgag
      661 cagctcgtcg tcggtcaccg ccgcgcctcg tcgaggagcc acagcacctc ccacgcgtcg
      721 agccggtgcg tctcggcgcc agccgcgagc ggacccgtcg cgggccacaa caggcgaccg
      781 cgcggcagct cgacccgcgg tccgtcgccg tcgtcgaggt gggcgatcaa ggtcagcttc
      841 gccccgtcgc ccagctccca ctcgacgcgc ggcgtgcgct cgccggcgag gttccaccga
      901 ccggagtggc ccgcgcaccc ggccaggcgc ggcacgatct cgcgccgccg cagctcgagt
      961 agcgtgcgat agtgggccag ccagcgtcgc gcgacggccg tctcgcgctc gtcgtcgtcg
     1021 agcaccgcgg actcgaacgt ggcgcgcagg ttcgggtcgg ggatccggcc gcgatcggcg
     1081 gcgcccgcga actcgacgaa ccgcgcgagc ccgcgccggc gcccttcggt caccgcgcgg
     1141 gccagctccg ggccgaaatc gcagaagaag ggaaacggtc ggcggcaccc gtactcctcg
     1201 cccatgaaca gcaacggcgg ggccggcgcg agcagcagca ccgcgaccgc cgccgcgagc
     1261 gatcgctcgc cggcgagcac ggtgagccgc tcgccgaagg cacgattccc cacctgatcg
     1321 tgattctgca ggaagccgac gaacgcggtc ggcggcaggt gtgcgctggc ctcgccacgg
     1381 ggtccgccgc ggtaggacga gtgctcgccc tgccaggcga acccctcggc gagcgcgcgc
     1441 gccaggcgcc gtgccggatc gacgacgaag tccgcgtagt acccgccgcg ctcgccggtc
     1501 agcaggcagt ggagagcgtg gtgcacgtcg tcgttccact gtgcgacgta gcatctcggc
     1561 gttcggtcgt cggcggcgcg acgcagccaa cgcgcctcgt tggcgacgtt ctcgagcacc
     1621 agatgcacgt gccgtcgcgc tcccgggccg gcctgcacgc gcgcggccag ttcgtcgaga
     1681 aagctcggtg aggactcgtc gtggatcgcg tgcacggcgt cgacgcgcaa tccgtccaga
     1741 tggtactcct cgagccagta gagcgcgttg tggatgaaga accggcggac ggtggcggaa
     1801 tccggcccgt cgaaccggat cgcgtcaccc cacggcgtcg tcgcgtcggg gtggaagaac
     1861 ggacgcgcgt accggtgcag gtagttcccc tcgggcccga agtggttgta gaccacgtcg
     1921 aggaagacca tcaggccgcg ccgatgcgcc gcggcgacga gccgcttcag ctcgtcgggt
     1981 ctgccgtagg acgagtccgg ggcgtagggc aggacgccgt cgtagcccca gttccgcgtt
     2041 cccggaaaat cggacaacgg catcagctcg atcgcggtga cgccgagcgc cgccagccgg
     2101 tcgagcccgc gacggacgcc gtcgtatccg ccctggcggc tgaacgcacc ggcgtgcagc
     2161 tcgtacagca ccgtctcctc ccacgggcgc ccgctccagc cggcgtcgtt ccaggcgaac
     2221 gatcgcggat cgatgacctc gctcggtccg tggacgtcac gcggctggta acgcgaggcg
     2281 gggtcgggga cgggcggccc gccgtcgacg cggtacccgt acagcgtccc cgcgccggcc
     2341 gggtgctcga gctgccagaa gccgtccgcc agccgatcgg tctcccgttc ctcgaccccg
     2401 tgccgcggat cgcccagcac cagatcgacg cgggccgcgc ccggcgccca cagccgaaag
     2461 cggacgccgt gcggcccgac ctccgcgccg aacggcatcg ccgccgcgcg gcgcaccacg
     2521 gcgtcagcca ccggcggcgg tccgaaagac caacgcggcg agcggcggca gcgtgacgtt
     2581 gatcgattgg aagtggccgt gcgccgcgac cggagccgtt tcgaccgcgc cgagattgcc
     2641 ctggccgctt ccgccgtaga tcgtcgcgtc gctgttgagc gcctcgtgcc agccgccggc
     2701 ccgcggcacc ccgagccgga agttcgatcg cggaaccggc gtgaagttga aggcgcagac
     2761 gatcgggcgc ttgcgctcgg cgtcccagcg caggaagctc agcgtgctct gcgcgctgtc
     2821 gttgcagtcg atccactgga acccgtcgga ctggaagtcg cgctggtgca gcgccggctc
     2881 gccgcggacg agcgtgttca ggtcccgcac ccaacgctgc aggccgcggt ggggcgcctg
     2941 ctcgagcagc ggccaatcga ggccgcggtc gtggtcccat tcccgccatt gcgcgaactc
     3001 gccgcccatg aacagcatca ccttgccggg atgcatccac atccagccgt acagcgtgcg
     3061 cagattggcg aagcgctgcc acgggtcacc cggcatcttg ccgatcaacg atcccttccc
     3121 gtggacgacc tcgtcgtgcg acaacggcag gacgaagttc tcgtggaagg cgtacatcat
     3181 gccgaaggtc atctggtggt ggtggaagtt gcggtggatc gggtccttgg cgatgtactg
     3241 cagcgtgtcg tgcatccagc ccatgttcca cttgagaccg aagccgaggc cgccgagata
     3301 ggtcggccgg gagaccatcg gccaggcggt cgactcttcg gcgatcgtga tcacgtcggg
     3361 gtgctgctgg tagaccgtcg tgttgaactg cttgaggaag ccgatcgcgt cgaggttctc
     3421 gcgaccgccg tacttgttgg ggatccactc gccttcgttg cgtgaatagt cgagatagag
     3481 catcgacgcg accgcgtcga cgcgcagtcc gtcgacgtga aacagctcga gccagaagag
     3541 cgcgttggcg atgaggaagt tccgcacctc gttgcgtccg tagttgaaga tcaacgtgcc
     3601 ccagtccggg tgttgcccct gacgcggatc ggcgtgctcg tacaggtggg tcccgtcgaa
     3661 gtagccgagg ccgtggtcgt ccttggggaa gtgcgccggc acccagtcga ggatcacgcc
     3721 gatgccgttc cggtgcaacg tgtcgatcag cgccatcaat tcttgcggcg tgccgaagcg
     3781 actggtcgcg gcgtagtagc cgatcgtctg atagccccac gagccgtcga acgggtgctc
     3841 gctgacgggc agcagctcga cgtgggtgaa gccgagccgg tgacagtagt cggcgagctt
     3901 gggcgccagc tcgccgtagc tcagccagcg acctcggtcg ccgtccgcgc gcatccacga
     3961 gccgaggtgg acttcgtaga tcgcgatcgg ggcgtcgagg gcgttccggc tccggcgggt
     4021 ctccatccag tccgcgtcgc cccacgagtg tccggacagg tcccagacga ccgacgccgt
     4081 cgccgggcgg gtctcgcagg cgaacgcgaa cggatcgctc ttttccaccc ggtagccgcc
     4141 gtgacgcgac cggatcgcgt acttgtagag cgcgccgtgg tccaccgcgg gcagaaagcc
     4201 ttcccacacg cccgagctgc cgcgcggctc gagccgatcg tgcgtctcgt cccagccgtt
     4261 gaaatcgccg accacagcga cccactcggc gttcggcgcc cagacggcga agcgggttcc
     4321 gcgacgaccg tcgcgctcga cgacgtgggc gccgaacttg tcccagatcc gccacagctt
     4381 accctcgccg aacaggtaca ggtcgtagtc ggtcaactcg ctcacgtcct cccctttcac
     4441 ggtttcaccg cgatcgagcg gatcgcctcg aacgggatcg gcagccagtc gggccgatgg
     4501 ttcagctcgt agcccaactc gtaaatcgcc ttgtccagca ggaacgcttc gagcagcgcc
     4561 tgcagcgcgt cggcctcgcc ggggacgatc ggcgagtcgc ccgccgattc gagataggcg
     4621 ccgagaaagg ccgcggcgac ccacgtctcc caggcgcgca cccacggctc gcgcaccgcg
     4681 atccgctcgg ggtgacggcc gacggcctcg atgaacgccg agtgcgccgc gtagccgaac
     4741 gagcggatca tccccgcgac gtccttcagc ggcgagtact tctcgcgccg ctcgccgatc
     4801 gtccgcagcg gctcgccctc gaagtcgagc agaacgaagt cgttttcggc gacgtgcagc
     4861 acctggccca gatggtagtc gccgtgcaca cggatccgcc acgcgtcggt acgcaggcga
     4921 tcgagcccgc ggaagcggcc gatcagcgcg gacgaatccg acagcaactc gtgcgcctgg
     4981 ctggcgacgc tcgcgggcag cgcggcgagg ttggcgttga gcgactcgac gacttgttcg
     5041 gcgcgccggg cgcagtcgtc ggccagcgcg cgcaggtagt ccgtcgtcat cgggatcggc
     5101 gcgaaggcgc cgtcgcgacg gtcgctcgcc agcgccaggt ggaactcggc ggtacggcgg
     5161 ccgagcccgc gcgcggcagc cagcgacgaa gcggcgcagc gctcgacgac caccggcggc
     5221 cggcgccgcg cgagcgccgg cagcggcgta cccgcatccg gcaacacggc ctcgcggcct
     5281 cccgtcgcga gcgcgcgctc gaggtagtcg gcgaacagcc gg
//
LOCUS       DASPGN010000590         4980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_814096, whole genome shotgun sequence.
ACCESSION   DASPGN010000590 DASPGN010000000
VERSION     DASPGN010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814096"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VD788_17080"
     CDS             <1..636
                     /locus_tag="VD788_17080"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HXV78026.1"
                     /translation="GPDGSHADECLRTIPPREHGGNMDIRYLQAGVTVYLPCYVDGCG
                     LAIGDPHYAQGDGEVSGTALEMDAVMTLVTEIVRGPEAPRLARGPHYEGPSRLLDIPS
                     RRFYATTGFPLKRAGELPPDMAYLESGKVAPLDNLSRDLSLAARQALLAMIDHIVAVY
                     GYTREQAYVIASIAVDLRIGQVVDSPNFGVTAILPLDIFEATGAGGRPGSP"
     gene            complement(688..1800)
                     /locus_tag="VD788_17085"
     CDS             complement(688..1800)
                     /locus_tag="VD788_17085"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXV78027.1"
                     /translation="MGPDHPEGSEADALSIPSLVSRVSTIDRRTANPWPTTNPTEIGL
                     AGTLIAVGIGLPGKEYLRYIAGPDGEGERDLVEKDSIPIVVQHTFGAPIDEVWQAIVD
                     PDRMRRWYFETIPDFVAETGFETQFTVRVDDRDYVHLWKVTEVTPGRKITYNWRYRGY
                     PGDSFVTWELSESPGGTKLTLTHDGIETFPQDNPIFSREAGHAGWSYFVTRSLRAFLK
                     GGKVMGSTAANDVIRVGPIEVRFRLQAGQTGGKLTMFEFLVPAQARVPVPHSHETFDE
                     TVYGLAGVATWELDGRQVRVRPGDVLFIPRGHVHHFANLEPQDARQLSVITPGLLGPD
                     YFREIAEVVNVGGPPNVERIMEVMRRHGLRPAPSAP"
     gene            complement(1901..2464)
                     /locus_tag="VD788_17090"
     CDS             complement(1901..2464)
                     /locus_tag="VD788_17090"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HXV78028.1"
                     /translation="MKKAILLLAALALIAAAHAVRAEEGQNDAQAAEKREDLDAMAQE
                     ALDKLFADNARATELYESAYGYAVFDNLKIALGLSGGGGSGVAVAKNGSERTYMKMGT
                     GGVGLGLGGQKYQVVFLFETENAFRSFVDKGWKAETGAQAAAGTKGASAEATFHDGLA
                     IFKMNDKGLMASADVSGTKYWKNKKLN"
     gene            complement(2518..4227)
                     /locus_tag="VD788_17095"
     CDS             complement(2518..4227)
                     /locus_tag="VD788_17095"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HXV78029.1"
                     /translation="MSKYSTVLAAWLVCALAGAPAFAQGAGELTERARTLLEQIEAKR
                     GEVRELRAQRDGLEGEERLLAERRLLRRAMETLDDLDALVDNVLAQEKRGLDATEFRE
                     PAESLVQLLAPLVRTRVDTLREEIAQLSTRRQTTTGEALIKLEDQLKELNRELVTVLR
                     AALDNAEHMEQLGLDASAERDYLVQTLSEKAESDAERFRLSVEQTKALATRVQAKPDD
                     ADLATELSALRAKQEVTQHSLTATIAMMERLELKTAEYHKLLIQGTGQITSEILDKEV
                     AVGLFQQWTERATTWVVDNGPRFVFKGLIFLVILLMFRLLARVVRRLVARAVAASKLN
                     LSQLLQKMVVSVSGNLVLGIGLLVALSQLGFALGPILAGLGIVGFILGFALQDTIGNF
                     AAGLMILIYRPYDVDDMIDAAGVFGRVSAMSMVSTTVLTIDNQTLIIPNGKIWGDVIK
                     NVTAQRIRRVDMTFGVSYEDDVARTEHVLAQILAEHPKVLKYPDPVIKLHSLGDSSVD
                     FVVRPWVKTDDYWDVYWDVTREVKMRFDREGIHIPFPQRDVHLYEQKRSAGIATGGEV
                     GVE"
     gene            4416..4940
                     /locus_tag="VD788_17100"
     CDS             4416..4940
                     /locus_tag="VD788_17100"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXV78030.1"
                     /translation="MMLRILVFVGVAALSLPGAMAGGYIGASAGQGSTSFDAGGGTNF
                     DASATSYKVLGGYRVLKFFGVEGDYRDFGSQRETVSGEEITLDTTAFDLFAVGVLPLG
                     LFEVFGKAGYTMWDAEVSALSQSVSDSGNDMAFGVGAGYAFGKVGVRLEYEMFDIADA
                     DDVSMVSVGAEFRF"
BASE COUNT          789 a         1680 c         1577 g          934 t
ORIGIN      
        1 ggtcccgatg gatcccacgc cgacgagtgc ctgcgcacga ttccgccgcg cgagcacggc
       61 ggcaacatgg acatccgcta cttgcaggcc ggcgtgaccg tctacctgcc gtgctacgtc
      121 gacggctgcg ggctggcgat cggcgacccg cactacgcgc agggcgacgg cgaggtgtcg
      181 ggcaccgcgc tcgagatgga cgcggtgatg acgctggtca ccgagatcgt ccgcgggccg
      241 gaggccccgc gtctcgctcg cggcccgcac tacgaggggc cgtcgcgcct gctcgacatc
      301 ccgtcccggc ggttctatgc cacgaccgga ttcccgctca agcgcgccgg cgaactgccg
      361 ccggacatgg cgtacctcga gtcggggaag gtcgcgccgc tcgacaacct gtcgcgcgac
      421 ctgtcgctcg ccgcacgaca ggcgttgctc gcgatgatcg accacatcgt cgcggtatat
      481 ggctacacgc gagagcaggc ctacgtgatc gcctcgatcg ccgtcgacct gcggatcggc
      541 caagtggtcg actcgccgaa cttcggcgtg accgcgatcc tgccgctcga catcttcgag
      601 gcgacggggg ccgggggtcg acccggttcg ccgtgacccg gcccggcccc tggttccggc
      661 agatccccag ttgcacgagc cgcgcggcta cggcgccgaa ggtgctggac gcagaccgtg
      721 ccgccgcatg acttccatga tccgctcgac gttcgggggt ccaccgacat tgaccacctc
      781 ggcgatctcc cgaaagtagt cggggccgag cagcccgggg gtgatcacgc tgagctgccg
      841 tgcatcctgc ggttcgagat tcgcgaagtg gtggacgtgt ccgcgcggaa tgaacaggac
      901 atcgcctggt ctcactcgga cttgccgccc atcgagttcc cacgtcgcta caccggccag
      961 cccatagacc gtctcatcga aggtttcgtg gctgtgcggc acaggcacgc gggcctgcgc
     1021 ggggacgagg aactcgaaca tcgtcagctt tcccccggtt tgcccagctt gcaaccgaaa
     1081 ccggacctcg atcggtccga cacggatcac gtcgtttgcc gcggtggaac ccatgacttt
     1141 gccccctttc aggaacgctc tcaggcttcg cgtgacgaag taggaccatc cggcgtgacc
     1201 cgcttcccgg ctgaagatcg gattgtcctg cggaaacgtc tcgatcccgt cgtgcgtaag
     1261 cgtcagtttc gtaccgccgg gcgactccga cagctcccaa gtgacgaacg agtctcccgg
     1321 gtaaccacgg tatcgccagt tgtaggtgat cttcctcccg ggagtcactt cggtcacctt
     1381 ccacaggtgc acgtagtccc ggtcgtcgac gcgcacggtg aactgcgtct cgaaccccgt
     1441 ttcggctacg aagtcgggga tcgtctcgaa gtaccaccgg cgcattcgat ccgggtcaac
     1501 gatggcctgc caaacctcgt cgatcggcgc gccgaacgta tgctgcacga cgatcgggat
     1561 tgaatctttt tccaccaagt ctcgctctcc ttcgccgtca ggccccgcga tgtaccgcag
     1621 gtactccttg cccggaagac cgatgccaac tgcgatcagc gttcccgcga gaccgatctc
     1681 ggttggattg gtcgtcggcc aggggttcgc tgttcttcga tcgatggttg acacgcgaga
     1741 aacaagagaa gggatggaaa gagcatctgc ttcgctccct tcgggatggt cggggcccaa
     1801 gttgcctcgt gtccatgacc cagagggccg gcgcgttggt tgctgcctgc attacacgga
     1861 actcggggcc gcgagatccc accggtatgg cgcgtgcggg tcagttgagc ttcttgttct
     1921 tccagtactt ggtgccggac acgtcggcgc tcgccatcag ccctttgtcg ttcatcttga
     1981 agatggccag tccgtcgtgg aacgtcgcct cggcgctcgc tcccttggtg cccgccgccg
     2041 cctgcgcccc ggtctcggcc ttccagccct tgtcgacgaa gctccggaac gcgttctcgg
     2101 tctcgaacaa gaagacgacc tggtacttct gaccgcccag accgaggccg acgccacccg
     2161 tccccatctt catgtaggtc cgctcgctcc cgttcttcgc caccgcgacg cccgagccgc
     2221 cgccgcccga gaggccgagc gcgatcttca ggttgtcgaa gaccgcgtag ccgtatgcac
     2281 tctcgtacag ctcggtggcc cgcgcgttgt cggcgaacag cttgtcgagc gcttcttggg
     2341 ccatcgcgtc gaggtcctcg cgcttctcgg ctgcctgcgc gtcgttctgg ccttcctcgg
     2401 cccggaccgc atgcgctgcc gcgatcagcg cgagcgctgc aagaaggaga atcgcctttt
     2461 tcacggtttg ctcctcatcg tgggtcgcac cggagccgcc tcgaccggaa ttctagccta
     2521 ttcgactccg acttcgcctc ccgtggcgat tccggccgac cgcttctgct cgtacaggtg
     2581 tacgtcgcgc tgcgggaacg ggatgtgaat gccttcgcga tcgaagcgca tcttgacctc
     2641 acgcgtcacg tcccagtaga cgtcccagta gtcgtccgtc ttgacccacg ggcgcacgac
     2701 gaaatcgaca gaagaatcgc ccaggctgtg cagcttgatc accgggtcgg ggtacttcag
     2761 caccttcggg tgctcggcca ggatctgcgc caacacgtgc tcggtccggg cgacgtcgtc
     2821 ctcgtacgac acgccgaagg tcatgtcgac gcgacgaatg cgttgcgccg tcacgttctt
     2881 gatcacgtcg ccccagatct tgccgttggg gatgatcagc gtctgattgt cgatcgtcaa
     2941 caccgtcgtc gacaccatgc tcatcgcgct gacgcgcccg aagacccccg cggcgtcgat
     3001 catgtcgtcg acgtcgtagg gacggtagat caggatcatc aatccggcgg cgaagttgcc
     3061 gatcgtatcc tgcagggcga agccgaggat gaagccgacg atgccgagac cggcgaggat
     3121 cggacccaag gcgaagccca gttgggaaag cgccacgagc aggccgatgc ccagcaccag
     3181 gttgcccgac accgagacga ccatcttctg caacagctgc gacaggttga gcttcgaagc
     3241 ggccaccgcc cgggcgacga gccgacgcac cacgcgcgcc agcaggcgga acatcagcaa
     3301 gatcacgagg aagatcagtc ctttgaagac gaatcgcggc ccgttgtcga cgacccacgt
     3361 cgtcgcccgc tcggtccact gctggaacaa cccgactgcg acctccttgt cgaggatctc
     3421 gctcgtgatc tgaccggtgc cctgaatcag cagcttgtgg tactcggccg tcttcagctc
     3481 gagccgttcc atcatcgcga tcgtcgcggt caggctgtgc tgggtgacct cttgtttcgc
     3541 gcgaagcgcg ctcagctccg tcgccaagtc ggcatcgtcc ggtttggctt gcacgcgcgt
     3601 ggcgagcgcc ttggtctgct cgacggacag ccgaaaccgc tcggcgtcgc tttcggcctt
     3661 ctccgacagc gtctggacca ggtagtcgcg ttcggccgac gcgtcgaggc cgagctgctc
     3721 catgtgctcg gcgttgtcga gcgccgcgcg cagcacggtc accaactcgc ggttcagctc
     3781 cttgagctgg tcttcgagct tgatcagtgc ttcgcccgtc gtcgtctggc gtcgggtcga
     3841 gagctgcgcg atttcttctc gtaacgtatc gacccgagtg cgaacgagcg gtgcgagcag
     3901 ctggaccagg ctttccgccg gctcgcggaa ctcggtcgcg tcgagtcctc gcttttcttg
     3961 ggccaacacg ttgtcgacca gagcgtcgag gtcatcgagc gtttccatcg cgcggcgcag
     4021 cagacgccgc tcggcgagta accgttcctc gccctcgagc ccgtcccgct gcgcgcgaag
     4081 ctcgcgaacc tccccgcgtt tcgcttcgat ctgctcgagc agcgtccggg cccgttcggt
     4141 gagctcgccg gccccctggg cgaacgccgg cgcgccggcc agcgcacaaa cgagccacgc
     4201 cgccagcacc gtcgagtact tgctcatcgc tccctccgag cgccctgtgc gtcgggtcga
     4261 cgcgaatgcc cgtgccgggc ggcgcgatcg tagcacacgg gctcgcgcgg aatccccggg
     4321 ttttcgccgc gggtttgcat ccccgccggc cgggatcgag attggaccgg gtcgtacaac
     4381 ctgggggcag acaggggtac ccgatgagac acaggatgat gcttcggatt ctcgtcttcg
     4441 taggcgtcgc cgcgctctcg ttgccggggg cgatggcggg cggctacatc ggcgcctcgg
     4501 ccggtcaggg cagcacgtcg ttcgacgccg gcggcggcac gaacttcgac gcctcggcca
     4561 cgagctacaa ggtgctgggt ggctaccgtg tactgaagtt cttcggtgtc gagggcgact
     4621 accgtgactt cggttcgcaa cgcgagaccg tctccggcga ggagataacg ctcgacacca
     4681 ccgccttcga cctgttcgcg gtcggtgtgc taccgctcgg actgttcgag gtattcggca
     4741 aggccggcta cacgatgtgg gacgccgagg tgagtgcgct gtcgcagtcg gtcagcgaca
     4801 gcggcaacga catggccttc ggtgtcggcg ccgggtacgc gttcggtaag gtcggcgtgc
     4861 gcttggagta cgagatgttc gacatcgccg acgccgacga cgtcagcatg gtatcggtcg
     4921 gagcagagtt ccgcttctga ttcccgtcga tcgaccggtc tgcctattcg agagcggccc
//
LOCUS       DASPGN010000591         4626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1751757, whole genome shotgun sequence.
ACCESSION   DASPGN010000591 DASPGN010000000
VERSION     DASPGN010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751757"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="VD788_17105"
     CDS             complement(<1..867)
                     /locus_tag="VD788_17105"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXV78031.1"
                     /translation="MNRNHMPGTVRHAEAGMSLIELLVSVTILAIAMTVALTIYQEAQ
                     RSYRQGENIVEQQQVSRVAFDLISNELRLAGLNLYPDGDHERPDEAIEAAFERAIVLR
                     ADFDRDEGSPSTDPEQALAGGKFVTISTGNDEIHGFVLAKSLPDGTIGGPDAIAFEAD
                     VAESPRDGEVEPVTIDDLVLSGDDPPYTLYRVSLNNDPAKYGDASFVTRTVLAENIRS
                     LRFRYFDLAGRELVAPGGAEDDASVAARAAIHRVTVELETLTRHPDPTTMTHRTWLLT
                     GRVTPRNRGRIRL"
     gene            complement(878..1348)
                     /locus_tag="VD788_17110"
     CDS             complement(878..1348)
                     /locus_tag="VD788_17110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78032.1"
                     /translation="MTPRAVRAGAAGFSLPEVTLALGLLAGLLISICGLFVLSSNLVH
                     GGRQRSSAVTMAENILEETNAWTFRGLFERFGYDGSAPSYVVDTRTNAAASGWQGEIA
                     ERLPSSHAEVVLESVAAGGSPPALRDATCIRVSVRVHWKDDLKWREATLTTVRM"
     gene            complement(1345..1839)
                     /locus_tag="VD788_17115"
     CDS             complement(1345..1839)
                     /locus_tag="VD788_17115"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HXV78033.1"
                     /translation="MRESDPVARRFSRGVTLPELLVVVSIISMAVAIAVPLIGSAIRS
                     ASARAATNGFAVNLRAARMIAVSNNTPVSVEVHTEPHPDCFCTPTFYEYPDRAGTMRR
                     FEMPRGITIAASTDPIVFLPNGSLPGGALTEIRVDNGDAAAEVWTVQCSVAGITSVVR
                     VGTR"
     gene            complement(2103..2912)
                     /locus_tag="VD788_17120"
     CDS             complement(2103..2912)
                     /locus_tag="VD788_17120"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HXV78034.1"
                     /translation="MTVFPEPFSSIVLLLLGLVFGSFLNVCIHRLPEGASVVRPRSSC
                     PECGSPIHWYQNVPVVSWLALGGRCAACRARISLRYPAVELLGGVLLVLLWRMLGPGL
                     AFPIAAFFALAMVVLFFTDFDHQILPDAVTLTGFAVGVAVAWLNPFLDGPGPRRLWLS
                     LAGAALGSGLLWGIGALYGRLRGVEAMGMGDVKMMAMIGAFTGPFGVMTTILFGSIGG
                     AVIGLLMIPLRGRTLQDALPFGCFLAPAAMVSLLYSGAVWSVYARILTPPL"
     gene            complement(2909..3760)
                     /locus_tag="VD788_17125"
     CDS             complement(2909..3760)
                     /locus_tag="VD788_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78035.1"
                     /translation="MTRRFGLIALVVLVAAATQFAAMRLHGMAGDREPSEQLLYLPNG
                     KYLELASLGQAPLLADLIYLWAIQYYAEYQPADRHRFVEHVFSDVIAELDPHFVDAYW
                     LGALILITEAGEFEEGIALLEQGFAANPDKWILAYLAAWECALAGQPIRAAGYFAQAE
                     AVDDAPPYISRMRAAMFARAGDLVEALGLWRGIYEDPDADPSSRAIAERKVRELTVQI
                     DLRALAAAVERFRADNRRYPETLADLRRRGYIRDVPRHPNGKAYDYDPRSGSVSAPRR
                     NAPAGSG"
     gene            complement(3757..4515)
                     /locus_tag="VD788_17130"
     CDS             complement(3757..4515)
                     /locus_tag="VD788_17130"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV78036.1"
                     /translation="MTAIAINTFREAIRDRVLYLLLVFALLLIGTSRLLSLLTVGSEL
                     KIIKDVGLSAISLFGALTAVFVGVSLVFKEIEKRTVYTLLANPVRRWQFIAGKFAGLV
                     TVLSLNVVMMFLALALVLAFWSESPWPLVPAVVLILVELAIVTAFALLFSTLTNPMLA
                     AVWTFAAYVAGHLSWSLLLLRDKLSPGFGQRACEVAFWLLPNLERLNIKAEVVHGLPL
                     PAGYVWIAIGYGAGYASVVLALACVVFERKEFLR"
BASE COUNT          843 a         1598 c         1550 g          635 t
ORIGIN      
        1 aagtcggatc cgtccgcggt tgcgcggcgt cacccgcccg gtcagcaacc acgtgcggtg
       61 ggtcatcgtc gtcgggtccg ggtgccgggt cagcgtctcg agctcgaccg tcacccggtg
      121 gatcgccgcc cgggcggcga ccgatgcgtc gtcttctgcc ccgcccggcg cgaccagctc
      181 gcgtccggcg aggtcgaagt agcggaagcg cagactgcgg atgttctcgg ccagcaccgt
      241 gcgggtgacg aagctcgcgt cgccgtactt cgccgggtcg ttgttcaggc tgacgcgata
      301 cagcgtgtac ggcgggtcgt cccccgacag gaccaggtcg tcgatcgtca ccggctcgac
      361 ctcgccgtca cgcggcgatt cggcgacgtc ggcctcgaac gcgatcgcgt cggggccgcc
      421 gatcgtgccg tcgggaagcg acttcgccag gacgaagccg tggatctcgt cgttgccggt
      481 cgagatcgtg acgaacttgc cgccggcgag cgcctgttcc ggatcggtcg acggggagcc
      541 ctcgtcgcgg tcgaagtcgg cgcggagcac gatcgctcgc tcgaacgcgg cttcgatcgc
      601 ctcgtcgggt cgctcgtggt caccgtcggg atagaggttg agcccggcga gccggagttc
      661 gttgctgatc aggtcgaacg ccacgcgcga gacctgctgc tgctcgacga tgttctcgcc
      721 ctgccggtag gagcgctgtg cttcctggta gatcgtcagc gcgaccgtca tcgcgatggc
      781 gaggatcgtc accgagacga ggagctcgat caggctcatc cccgcttcgg cgtggcgcac
      841 ggtgccgggc atgtggttcc ggttcacggc acgcccctca catccggacg gtcgtcagcg
      901 tggcttcgcg ccatttcaag tcgtctttcc aatggacccg caccgaaacc cggatgcacg
      961 tcgcatcgcg cagcgccggt ggcgaaccac ccgcggcgac cgattcgagc acgacctcgg
     1021 catgggagga cggcagccgt tccgcgatct cgccctgcca gccgctcgcg gccgcgttcg
     1081 tccgcgtgtc gacgacgtag ctcggcgcgc tgccgtcgta gccgaacctc tcgaacaacc
     1141 cgcggaaggt ccaggcgttg gtctcttcga ggatgttctc ggccatcgtc accgccgacg
     1201 accgctgccg gccgccgtga accaggttcg acgacagcac gaacaacccg cagatcgaga
     1261 tcagcagacc ggccaacagg ccgagcgcga gcgtgacctc gggcagcgag aaaccggccg
     1321 cgccggcacg aactgcacgg ggggtcatcg cgtccccaca cgcacgaccg aggtgatgcc
     1381 ggccacgctg cattgcaccg tccacacctc ggcggcggcg tctccgttgt cgacccggat
     1441 ctcggtgagc gcgccacccg gaagcgagcc gttcggcagg aagacgatcg ggtcggtcga
     1501 cgccgcgatc gtgatccccc gcggcatctc gaaccggcgc atcgtgccgg cccggtcggg
     1561 gtactcgtaa aacgtcggcg tgcagaagca gtcgggatgc ggctcggtat gcacttcgac
     1621 ggagaccggc gtgttgttgc tgaccgcgat catccgcgca gccctcaggt tgacggcgaa
     1681 tccgttggtc gcggcacgtg cgctcgccga gcggatcgca ctgccgatca gcggtacggc
     1741 gatcgcgacg gccatcgaga tgatcgagac cacgacgagc aactcgggaa gcgtgacgcc
     1801 ccgagagaat cgccgtgcga ccggatcgga ttctcgcatc cccgcgcgca ctccttcgtc
     1861 ttgccgaccg accggacgcg ggctcgccgc gctccgcggg agtttccccg aacgtcttcg
     1921 cgaccggaac ccgaacggac cgcagctccg ggccgacccg tttgccagct accgtaagcg
     1981 aaaaccctcg ctcgatcaag accggattga acatgtcgga acatacgcgc cggggcgcgc
     2041 tgcgggagga ccgaagaggg accgccgtcg gtcgacgggc tcgacctaga acaaatgtaa
     2101 gctcagagtg gcggagtcag gatgcgggcg tagaccgacc aaacggcgcc ggagtacagc
     2161 aacgagacca tcgccgccgg tgccaggaag cagccgaacg ggagcgcgtc ctgcagcgta
     2221 cgcccgcgga gcgggatcat cagcagaccg atgaccgccc cgccgatcga gccgaagagg
     2281 atcgtcgtca tcacgccgaa cggcccggtg aacgccccga tcatcgccat catcttgacg
     2341 tcgcccatcc ccatcgcctc gacgccgcgc aacctgccgt agagcgcccc gatcccccac
     2401 agcagccccg agccgagggc cgctcccgcc agcgacagcc acagacgccg cgggcctggc
     2461 ccgtcgagga acggattgag ccaggcgacc gccactccga cggcgaagcc cgtcagcgtc
     2521 accgcgtccg gcaggatctg gtggtcgaag tcggtgaaga acagcacgac catcgccagc
     2581 gcgaagaaag cggcgatcgg aaacgcgaga ccgggcccga gcatgcgcca cagcaggacc
     2641 aacagcacgc cgccgagcag ctcgaccgcc ggataacgca gcgagatgcg agcgcgacac
     2701 gccgcgcacc ggccaccgag cgcgagccac gagacgaccg ggacgttctg ataccagtgg
     2761 atcggcgaac cgcactccgg acaactcgat cgcggacgca cgacgctcgc gccctcgggc
     2821 aagcgatgga tgcagacgtt gagaaagctg ccgaatacca gccccaacag cagcaagacg
     2881 atcgaggaga acggctcggg aaacacggtc atccggaccc cgccggtgcg ttccgacggg
     2941 gtgcggaaac ggagccggac cgcgggtcgt agtcatacgc tttgccgttc gggtggcggg
     3001 gtacgtcgcg aatgtacccg cgccggcgca ggtcggccag cgtctccggg taacgccggt
     3061 tgtcggcacg aaacctctcg acggccgccg ccagtgcgcg cagatcgatc tgcaccgtca
     3121 actcgcgcac tttgcgctcc gcgatggcgc gcgagctggg gtcggcgtcg ggatcctcgt
     3181 agatcccgcg ccacagtccg agcgcctcga ccagatcgcc ggcgcgggcg aacatcgcgg
     3241 cgcgcattcg cgagatgtag ggtggcgcgt cgtcgaccgc ctcggcctgc gcgaagtatc
     3301 cggccgcgcg gatcggctgt ccggcgagcg cgcattccca ggccgccagg taggccagga
     3361 tccacttgtc cgggttggcg gcgaagcctt gctcgagcag cgcgatcccc tcctcgaact
     3421 cccccgcctc ggtgatcagg atcagcgcgc cgagccagta ggcgtcgacg aagtgcggat
     3481 cgagctcggc gatcacgtcg gagaagacgt gctcgacgaa gcggtgccgg tcggcgggct
     3541 ggtattcggc gtagtactgg atcgcccaca ggtagatcag atcggccaac aacggcgcct
     3601 gcccgagact ggccagctcg aggtacttcc cgttcggaag atagagcagt tgttcggacg
     3661 gttcgcggtc ccccgccatc ccgtgcaagc gcatcgcggc gaactgggtc gcggccgcga
     3721 ccaacacgac gagcgcgatc aggccgaagc gacgcgtcac ctcaggaact ccttgcgctc
     3781 gaagacgacg caggcgagcg ccaggacgac ggaagcgtag cccgctccgt agccgatcgc
     3841 gatccagacg tagcccgccg gcagcggcaa gccgtgaacg acctcggcct tgatgttgag
     3901 tcgctcgaga ttgggcaaca accagaacgc gacctcgcac gcccgctggc cgaagccggg
     3961 cgacagcttg tcccgcagca gcagcaagct ccacgacagg tgaccggcga cgtacgcggc
     4021 gaaggtccaa accgcggcga gcatcgggtt ggtcaacgtc gagaacagca gcgcgaacgc
     4081 ggtcacgatc gcgagctcga cgagaatcag caccacggcc ggcacgagcg gccacggcga
     4141 ctcgctccag aaggccagca ccaaggccag cgcgaggaac atcatcacga cgttcaacga
     4201 caggaccgta acgaggccgg cgaacttgcc ggcgatgaac tgccaacgcc ggaccgggtt
     4261 ggcgagcagc gtgtagaccg tccgcttctc gatctccttg aacacgagcg acacgccgac
     4321 gaaaaccgcc gtgagcgcgc cgaacagcga gatcgccgac agtccgacgt ccttgatgat
     4381 cttgagctcg ctgccgacgg tcaacagcga cagcagtcgc gaagtgccga tcagcagcag
     4441 ggcgaagacc agcagcaggt agagcacgcg gtcgcggatc gcctcgcgaa aggtattgat
     4501 cgcgatcgcc gtcaccgcgg cgcccgctcc gggcccgccc gtacgggaag tggcgctcgc
     4561 tccgctcacg agcgctcccc gacggaatgg ccgatctcgc gcaggaacag gtcctcgagc
     4621 gtctcc
//
LOCUS       DASPGN010000592         4871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_580026, whole genome shotgun sequence.
ACCESSION   DASPGN010000592 DASPGN010000000
VERSION     DASPGN010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580026"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..521)
                     /locus_tag="VD788_17135"
     CDS             complement(54..521)
                     /locus_tag="VD788_17135"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV78037.1"
                     /translation="MQIDGGSGGGGLQSEINVTPLVDVVLVLLIIFMVIIPLTIRGYD
                     VDIPRASFDVAPPEPRNEQVVLAIDAATCPIVDPPGAAGLPADCVVRLNDDRVGIAEL
                     AARVGEIFAQREAEHRVLFLAAQDRLNYEGVMRVIDVAKSGVDGLRIGLVTGG"
     gene            complement(524..940)
                     /locus_tag="VD788_17140"
     CDS             complement(524..940)
                     /locus_tag="VD788_17140"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXV78038.1"
                     /translation="MQVGEPEKLRSTINVTPLVDVVLVLLIIFMVMAPQLQSGPPVRL
                     PETDRPEDSSDPTRQIRVTLETDGTFWVDDQRLTADQFPEFLGRTAAERGDLKVVLRG
                     DARLTYGEVKRAMLIIESAGFNDVGLVAEKRAAEGS"
     gene            complement(927..1598)
                     /locus_tag="VD788_17145"
     CDS             complement(927..1598)
                     /locus_tag="VD788_17145"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXV78039.1"
                     /translation="MHFSAILGSMGVVGVAVMVSLLALSVYSVTLIFDKLRRFRAASS
                     QSRQFLPLFGNCMRQGKLRDAIHTAQQHDKSHVAKVVAAGVTEFVGTGGDSDTRVEMV
                     ARALDRSRVQTLTEMKRGLGGLATIGSTAPFIGLFGTVVGIINAFRGISASGSGGVAA
                     VSGGIAEALIATALGIFVAIPAVVAFNHFTGALERFQVEMNSASSELVDFLHKATRQA
                     VHAGR"
     gene            complement(1608..3296)
                     /locus_tag="VD788_17150"
     CDS             complement(1608..3296)
                     /locus_tag="VD788_17150"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HXV78040.1"
                     /translation="MRKTSRVVRAALASALFVALSAPAPAAEEPGQLDAVLDEIVARE
                     RELTRTLEQYRPLIETYMQTVEPDVELGLVPVRDRYFLGRLSLGDAGAVEAEKKKKKR
                     TRSKLVDLKNDLYSASFKADGFSQVMVLDRGSFDRETYDFFFVRREFIGEIRTLVFDV
                     APKHNATGRFTGRIWVEDRDHHIVRYNGLYTSILDTSFRFDSWRLNMAPGLWLPAYVY
                     TEKAEHASSKKRARSNDAPSAKHTSQTRIWGYQVNGRTEQEEFTKVLVDAPLTRDRVD
                     APGQVSPVESMRAWEREAEENVVRRLERAALLAPEGPVDQVLETVVANLEVTNGLAIE
                     PPIRCRVLMTTPLESFTVGHTIILSRGLIDVLPDEASLAMVLAHEMGHVLSGHRLDTT
                     YAFSDQMLLGDREAMEEFVFERHPEEEREADARAIALLQNSPYKDKLANAGLFLKALA
                     TKSAALPSLIRAHFGNRLADAQTLVRMNEVMRGAPELDETSLEQIAALPLGGRVKVDP
                     WSARIELMTNNGVALVSAREKMPFMVTPLMPYLARYGADGASEENQNEMASSAD"
     gene            complement(3313..4380)
                     /locus_tag="VD788_17155"
     CDS             complement(3313..4380)
                     /locus_tag="VD788_17155"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXV78041.1"
                     /translation="MLTGARSREPGARLRPVAASVAVHLVVMTLALLLPARLFPVSNK
                     PEPVEVVFYRPDTLPPPPAPPVARVEPPEPLPQPEPIAEPVPEASRPLRPEPAAITRV
                     EPPRPKPVPRLARPEPTPVAPKREVRTDVFAHAERKAPRPTTSPKQTEVVGFGAAEPV
                     APQSTARVRTEAKVGGFAVAAAGAPPAERRERVVEVAGFAAPEESPGPRRPRAEERTV
                     TATAFVAAPTETAAGPPREASGAITQGRFGDGYSIARAPGTRQRPASDPDTPVEIIAK
                     PKPVYTDAARSGGIEGEVVLEVVFNASGRLDVLRVLGSLGFGLDEAAVEAAKKIRFKP
                     ARRDGKPIDHVALLTIVFQLA"
     gene            4661..>4871
                     /locus_tag="VD788_17160"
     CDS             4661..>4871
                     /locus_tag="VD788_17160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78042.1"
                     /translation="MERPFDPDVPIERALTPPSAWYTDPAIHQTELGRVFRHAWQPVG
                     RVEQLRSAGDFLSGDLFGEPFLVVLD"
BASE COUNT          753 a         1695 c         1632 g          791 t
ORIGIN      
        1 cgaaatcgaa cgagtggcag gttagcagga acgtgcggag ggagcaagac ggatcagccg
       61 cccgtgacga gaccgatccg caatccgtcg acgcccgact tggcgacgtc gatcacgcgc
      121 atcacaccct cgtagttcag tcgatcctgc gccgcgagga acagcacccg gtgctcggcc
      181 tcccgttgcg cgaagatctc gccgacccgt gccgcgagct cggcgatccc cacccggtcg
      241 tcgttcaatc gcacgacgca atccgcggga agaccggccg cccccggcgg atcgacgatc
      301 gggcaggtcg ccgcgtcgat cgcgagcacc acctgttcgt tgcgtggctc gggtggtgcg
      361 acgtcgaacg aggctcgcgg aatgtcgacg tcgtagccgc gaatcgtcag cgggatgatc
      421 accatgaaga tgatcagcag caccagcacc acgtcgacca gcggcgtgac attgatctcc
      481 gattgtaggc cgccgccgcc gcttccgccg tcgatctgca tcgtcagctt ccttccgccg
      541 cgcgcttctc cgccaccagc ccgacgtcgt tgaagcccgc gctctcgatg atcagcatcg
      601 cgcgcttgac ctcgccgtac gtcaggcgtg cgtcgccgcg caggacgacc ttgaggtcgc
      661 cacgctcggc ggccgtccga ccgaggaact ccgggaactg gtcggccgtc aaccgttgat
      721 cgtcgaccca gaacgtaccg tccgtctcga gcgtcacgcg gatctgccgc gtcgggtccg
      781 acgagtcctc cggccggtcg gtctccggga gccggaccgg cggtccggac tgcagctggg
      841 gcgccatcac catgaagatg atcaacagca ccagcaccac gtcgaccagc ggtgtgacgt
      901 tgatcgtcga ccggagcttt tccggctcac cgacctgcat ggaccgcctg tctcgtcgcc
      961 ttgtgcagga aatcgaccag ctcggacgag gcgctgttca tctcgacctg gaaccgctcc
     1021 agcgcgccgg tgaagtggtt gaacgccacg accgcgggga tcgcgacgaa gatcccgagc
     1081 gccgtcgcga tcagcgcctc ggcgatgccg cccgaaaccg cggctacgcc gcccgagccc
     1141 gaggcggaga tgccgcggaa ggcgttgatg atgccgacga ccgtgccgaa cagcccgatg
     1201 aacggcgccg tcgatccgat ggtggcaagg ccgccgaggc cgcgcttcat ctcggtcagt
     1261 gtctgcaccc gcgagcgatc gagcgcgcgc gccaccatct cgacgcgagt gtcgctgtcg
     1321 ccaccggtac cgacgaactc ggtcactccg gcggcgacga ccttggccac gtgcgacttg
     1381 tcgtgctgct gggcggtgtg gatcgcgtcg cgcagcttgc cttgcctcat acagttgccg
     1441 aacagcggaa ggaactggcg agattggctg gaggcggcgc ggaaccgccg caacttgtcg
     1501 aagatcaacg taaccgagta gacggacaac gccagcaacg agaccatcac ggccacgccg
     1561 acgacgccca tgcttcccaa gatcgctgaa aagtgcatct ctggctccta gtcggcggac
     1621 gacgccattt cgttctgatt ctcctcggac gcgccgtcgg cgccgtatct ggcgaggtac
     1681 ggcatcaacg gcgtgaccat gaacggcatc ttctcgcgtg cggagaccag cgcgacgccg
     1741 ttgttcgtca tcagctcgat ccgggcgctc cacgggtcca ccttgacccg tccgccgagc
     1801 ggcagcgcgg cgatctgctc gagcgaggtc tcgtcgagct cgggcgcacc gcgcatcact
     1861 tcgttcattc ggacgagcgt ctgtgcgtcg gccagtcgat tgccgaagtg ggcgcggatc
     1921 aaggacggca gcgccgccga cttggtggcc agcgccttga ggaacagacc ggcattggcc
     1981 agtttgtctt tgtacgggga gttctgcaag agcgcgatcg cccgggcgtc ggcctcgcgt
     2041 tcctcctccg gatgacgctc gaagacgaac tcctccatcg cctcgcggtc gccgagcagc
     2101 atctggtcgc tgaacgcata agtcgtatcg agacgatggc cgctgagcac gtgacccatc
     2161 tcgtgcgcca gcaccatcgc caggctcgcc tcgtccggca gcacgtcgat cagcccccgg
     2221 ctgaggatga tcgtgtgacc gaccgtgaac gactcgagcg gtgtggtcat caacacgcga
     2281 cagcgaatcg gcggctcgat cgccaggccg ttggtcacct cgaggttggc gacgacggtc
     2341 tcgagcacct ggtcgaccgg tccctcgggc gccagcagag cggctcgctc gagccggcgc
     2401 acgacattct cctcggcctc acgctcccag gcgcgcatgc tctcgaccgg cgagacctgg
     2461 cccggtgcgt cgactcgatc gcgggtcaac ggcgcgtcga ccagcacctt ggtgaactct
     2521 tcctgttccg tacggccgtt gacctgatag ccccagatgc gggtctggct cgtgtgcttg
     2581 gccgacggcg cgtcgttcga ccgagcacgc ttcttgctcg aagcgtgctc ggccttttcg
     2641 gtgtagacgt aggccggcag ccacagcccg ggcgccatgt tcagtcgcca gctgtcgaag
     2701 cggaagctgg tgtcgaggat cgaggtgtac aggccgttgt agcgcacgat gtggtggtcg
     2761 cggtcctcga cccagatgcg gccggtgaaa cggcccgtcg cgttgtgctt cggcgcgacg
     2821 tcgaagacca acgtgcggat ctcgccgatg aactcgcggc gcacgaagaa gaagtcgtag
     2881 gtctcccggt cgaagctgcc tcgatcgagc accatcacct gtgaaaaccc gtcggccttg
     2941 aagctcgcgg agtacaggtc gttcttcaga tcgaccagtt tgctccgcgt gcgcttcttt
     3001 ttctttttct cggcttcgac cgcgcccgcg tcgccaagcg acagccggcc gagaaagtag
     3061 cggtcgcgca ccggcaccag ccccaactcg acgtcgggtt cgaccgtctg catgtaggtc
     3121 tcgatcaatg gccggtactg ctcgagcgtc cgcgtcaact cgcgctcgcg cgcgacgatc
     3181 tcgtccagca cggcgtcgag ctggcccggt tcctccgcgg cgggcgccgg ggccgacaac
     3241 gcgacgaaca gcgccgacgc cagcgccgcg cggaccacgc gtgaagtctt tctcacggta
     3301 acctccatcc ctctaagcaa gctgaaacac gatggtgagc aaggcgacgt ggtcgatcgg
     3361 ctttccgtcg cggcgggccg gcttgaaccg gatcttcttc gccgcttcga ccgcggcctc
     3421 gtccagcccg aaccccaagc tgccgaggac acgcagtacg tcgagccgac ccgaggcgtt
     3481 gaagacgacc tcgagcacga cctcgccctc gatgccgccg cttctcgccg cgtcggtgta
     3541 taccggcttg ggcttcgcga tgatctcgac cggcgtgtcg gggtccgacg ccggacgctg
     3601 gcgggtcccg ggcgccctgg cgatcgagta gccgtcgccg aaccgtccct gggtgatggc
     3661 cccggacgcc tcccgcggcg gtccggcggc ggtctcggtc ggcgcagcga cgaaggcggt
     3721 ggcggtgacc gtgcgctcct ccgctctcgg ccggcgcggg cccgggctct cctccggagc
     3781 ggcgaaaccg gcgacttcca ccacccgttc ccgtcgctcg gccggcggcg cgccggcggc
     3841 cgccaccgcg aagccaccga ccttggcctc cgtccggacc cgcgccgtcg actgcggcgc
     3901 gacgggctcc gcggcgccga aaccgacgac ctcggtctgc ttcggggacg tcgtcgggcg
     3961 cggcgccttt cgctcggcgt gggcgaagac gtcggtccgc acttcgcgct tcggcgcgac
     4021 cggcgtcggc tcgggccggg ccaggcgcgg gacgggcttc ggtcgcggcg gttcgacccg
     4081 cgtgatcgcc gcgggctccg gccgcagcgg tcgactcgct tcggggacgg gctccgcgat
     4141 cggctcgggc tgcggcaacg gctcgggtgg ttcgacgcgt gcgacgggag gcgcgggggg
     4201 agggggcagg gtatcgggtc ggtagaacac cacctcgacc ggttccggct tgttcgagac
     4261 cgggaacaac cgtgccggca acagcagtgc cagcgtcatg acgaccaagt ggacggcgac
     4321 gctcgccgcg accggccgca gccgagcgcc gggctcgcgc gaacgggctc cggtcagcac
     4381 gaggtccgtc cggaacgaga cggccggccg gggagcgcgg acgggctgcc gcccgccggc
     4441 caccggtggc gtggtcgcgg ttacgcttct gtcgctacgg gacgtcgttg tcgtaaccaa
     4501 cggtccaccc acctcgtccg cgacggcccg tgggaatctg cgccgccgcg tgattccgcg
     4561 acgcgccgga ccgttcgcgc gcgtccgacc agtgcagaaa ccgggccaac tcggatcgga
     4621 cggtcgacgc gctgtgctag atttcggctc gaggatcgct gtggaacgac cgttcgaccc
     4681 cgacgttcca atcgagcggg cgctgacgcc gccgtcggcg tggtacaccg acccggcgat
     4741 ccaccagacg gagctcgggc gcgtcttccg tcatgcgtgg cagccggtcg gacgcgtcga
     4801 acagctacgg agcgcgggcg acttcctttc gggcgatctt ttcggcgagc cgtttctcgt
     4861 cgtcctcgac g
//
LOCUS       DASPGN010000593         8737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1282564, whole genome shotgun sequence.
ACCESSION   DASPGN010000593 DASPGN010000000
VERSION     DASPGN010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8737
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282564"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VD788_17165"
     CDS             complement(<1..255)
                     /locus_tag="VD788_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78043.1"
                     /translation="MAGIAFIWLVALASFTPDTLSADGDAVIGRWRTEPEEHGYAHVE
                     IRREADRYVGEIVWLSEPDAPGPDPRVPSGRPKTDYNNPDP"
     gene            365..2026
                     /locus_tag="VD788_17170"
     CDS             365..2026
                     /locus_tag="VD788_17170"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HXV78044.1"
                     /translation="MRAILFSVVLATSLLVTGCGDGARPAERPTETLRGLQLDATRGG
                     RPEILRWGPDPQRSVSWHHHSNRLIPVYAFGTRGGGDGLDLDTVGGASSVYRARDALA
                     ALYGRHPERTLNARADYFDQTDIARIQRAGVAAGKRYLFLVVFDGMDWQTTRAASIYR
                     TGEVRYDRGRGSGLHFQDYDASGSSQFGFMVTSPANDRTAGDPGTQRVSDRGSGLYGG
                     YDASRGGAEPWDEGPEPAYVIAEDFSNPTRHAYTDSAAAATSMCSGIKTYSAAVNVDT
                     AGRQQLPIARELQQRGFGVGVVTDVPISHATPAAAYANNVVRHDYQDLSRDMLGLPSI
                     AHPRRALAGLDVLIGCGAGVERATDAAQGDNYVPGNRYLASDDLRAIDEAGGGDYVVV
                     VRERGTDAGRALEHGARRAAERGLRLFGFFGTTYAHLPYRTADGGHDPVEGQDGQAER
                     YSAADLVENPTLAEMTAAALVVLGSRAEGFWLMVEAGDVDWANHDANIDAAVGSVLSG
                     DEAVRVITDWVETHSDWTESLLVVTADHGHYPVLVDPERFASFRP"
     gene            complement(2035..2937)
                     /locus_tag="VD788_17175"
     CDS             complement(2035..2937)
                     /locus_tag="VD788_17175"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXV78045.1"
                     /translation="MTIIRSFVGSLVALSCYGLVAAQVTIDRSLPAPKQPEVVVENMF
                     GTIEIRAWSKNEVRVSGRLAPGAEGLAFDADQGEFHISVEAPEHWLYESDDDTDYRSQ
                     LVVELPTGSSVGVRSLNASIRIDGVNGLIEVESVNGAVSIGGDPRVVRIESMTGTVDV
                     QAVAAAMQVESVSGDVTLVGATRAVEVETISGGVILTGSSFEEVSIETVSGSVSIEGS
                     LTAQGGIEVETHNGTVEIVVPAATRARFSFSTFNGSIENTIGPSPRKTGRFEPYTELR
                     FSTGSEDFEVSVETFSGNISLRTN"
     gene            complement(2948..3655)
                     /locus_tag="VD788_17180"
     CDS             complement(2948..3655)
                     /locus_tag="VD788_17180"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXV78046.1"
                     /translation="MTDCQWTLDRLDDYLDGELARDETSAIRRHLADCTRCCSSERAL
                     RGLLERAASLPRAIDPPRELWPEIERRLAVAGRRAGGPGATWGRPTKGAWISGLLAAS
                     LLAVGLAAGYLLRGTELRPTQVPARASSSSPAIRTAVGAGDLVLAEQAIVEAERELRT
                     LLDERPGALSAETVRTVERNLAVIEAAIDEIQTVLARDPDNRDLGRRLLTARQRQLNL
                     LQRATQLGARPVATDSI"
     gene            complement(3652..4200)
                     /locus_tag="VD788_17185"
     CDS             complement(3652..4200)
                     /locus_tag="VD788_17185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV78047.1"
                     /translation="MPEAKDERDSDEWEWVLGAQRGDRRCFEQLYRRHCGSIYALCLR
                     LGRRSHEAEEMTQETFVRAWQHLDTFREPGHFGAWLNRVAVNLTLSARRRRSRRGEPL
                     PLFEEFVPTGETASGAGGESRVDLDRAIACLPDGARTVFVLHDVHGYRHHEIATMTGI
                     AIGTAKAQLHRARKRLRELLDK"
     gene            complement(4311..5387)
                     /locus_tag="VD788_17190"
     CDS             complement(4311..5387)
                     /locus_tag="VD788_17190"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXV78048.1"
                     /translation="MLVLILFVAFALGVSFLCSLLEAALLSARTARLIEKRAAGVIGA
                     GLLLTLKQSRLDDAISAVLILNTIANTLGATLAGAQAAQVFGRTWVGVFSGLLTFVIL
                     IVSEIIPKTLGAVYSTQLSGMVGWTLTGLVKLMSPALFLSRVITRLLARGGGASLSRS
                     ELATVISMATREGVLSPEESRMFANLLRFDSIQVEDVMTPRTVTFMLPADATIADLLA
                     EAEARFFSRIPLFRPDRDHVVGYVLQRDVLKAVAEHCDREQPLERFMRPISHIPELAS
                     VGAALRRILKQREPIAVVTDEHGGVAGLVTLEDLTETILGTEIVDESDQVADMRVSAT
                     KLRDQRLERIRKMRQPATTGENDG"
     gene            5585..6403
                     /locus_tag="VD788_17195"
     CDS             5585..6403
                     /locus_tag="VD788_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78049.1"
                     /translation="MTQLVVDLACAEGRSVVQHLQERCMVRINGNRRACAAPRTERRR
                     LLAGCAAVALLGGVATAEEVRIEPLRLPEVRLETAAPAERLRAAGDESFLPRALSTGY
                     PRRVQRGFYDTHLLPELAHQMNGFRSLHGLPGSAAEIEQSLIYSSVGDSARRKTIKGA
                     TNALKDYLLEVTELTTVIGSVPTPRFGGGEVAGDAARRQRAELGFSFGFAHRMPNLGV
                     KYRIGSTLTRLGLTGHGAVDLRIEREDATRMRSSIGFNYDPRDNWYGMNYSVRF"
     gene            complement(6406..8121)
                     /gene="metG"
                     /locus_tag="VD788_17200"
     CDS             complement(6406..8121)
                     /gene="metG"
                     /locus_tag="VD788_17200"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HXV78050.1"
                     /translation="MPTKRYLVTSGLPYSNGLLHVGHVAGAYLPADTYVRYRRARGDE
                     VLFVCGSDDNGVSSLIAARKEGQSVEELTAHYHARQREDFASLGIEFDIFGGTHQPAF
                     VERHVEMSQAFFRTIHQRGYFNKRETEQLYDPEARQFLPDRFVRGRCYHARADGSPCD
                     NPEANGDQCESCGNAIDPLRLIDPVSTISGARPERRSTVHWYLALGRFERPLRDWLES
                     KRQAGPGDPAWREWVLNFTLGQINRGLPERAMTRDLAWGVPVPLDDPDAAGKVLYVWF
                     DAPIGYVSFTAALCVERGGGASDYERWWKSEESRIIHFIGEDNTVFHALIWPAMLMAE
                     GSYRLPWQVVANSFLNLGGEKMSKSRGTALWINNYAARFDPDPLRYYLTAIAPENARA
                     SFDPDDFVARNNGELLNALGNFVNRTLTFAQRYCDGRVPDPGDRGEIDSEHLALMRQQ
                     HDATTDHLEAFRFKSALAAVMTLARSSNRYLDRKQPWKQRALDAAGCRTTVNVCIQTV
                     RALTVLMAPFLPFSARETAKMLGLEQSDLAWDAALVELPPGHRLGQPRILFRKLEAAD
                     LERWA"
     gene            8220..>8737
                     /locus_tag="VD788_17205"
     CDS             8220..>8737
                     /locus_tag="VD788_17205"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HXV78051.1"
                     /translation="MLRHPANEGQAGSQSAVLFDKRLLPKRFVVVSNRLPYQLDMTDG
                     EVRFKRGIGGLVTALDPILSLTGGTWVGWTGSYDRLPPRVEVQADGTGQRPYDLVPVR
                     LDPRQVEQYYLGYSNRCLWPLFHYFQEHCEFNRDHWEVYCEVNREFADAIVDCHEDGD
                     MIWVHDYHLMLLP"
BASE COUNT         1406 a         2994 c         2943 g         1394 t
ORIGIN      
        1 cgggtccggg ttattgtagt cggtcttggg gcgtccgctg ggaacgcgcg gatccgggcc
       61 gggcgcgtcc ggttcgctca gccagacgat ctcaccgacg tagcggtccg cctcccgccg
      121 gatttcgacg tgggcgtagc cgtgctcctc gggctccgtg cgccagcggc cgatgacggc
      181 gtcgccgtcg gccgacaggg tatccggggt gaatgacgcc agagcgacga gccagatgaa
      241 ggcaatcccg gccattcgat cctcttcggg cgacggtcgg cgtccagtct agccccggtc
      301 ccgcgtgcgg gcgaaccgcc agcgcgatgg agtggcgcgc ggagttgctc taggatggcg
      361 gccgatgcgc gcgattctct tctcggtcgt cctcgcgacg tcgctgctgg tgacggggtg
      421 cggcgacggg gcgaggccgg ccgagcgccc gaccgagacg ctgcgcgggt tgcagctcga
      481 tgccacgcga ggcggccgtc ccgagatcct gcgctggggt ccggacccgc agcgcagcgt
      541 gtcctggcac caccattcga accggctgat cccggtctac gcgttcggca cccgcggcgg
      601 cggcgacggt ctcgacctcg acaccgtcgg cggcgcgtcg agcgtgtatc gcgctcggga
      661 cgcgctcgcg gcgctgtacg gccgccaccc cgagcgaacg ctcaacgcgc gcgccgacta
      721 cttcgaccag accgacatcg cacgaatcca gcgcgccggc gtggcggcag gcaagcgcta
      781 cctgtttctc gtcgtcttcg acgggatgga ttggcagacg acgcgggccg cgtcgatcta
      841 ccgcaccggc gaggtgcggt acgaccgggg gcggggcagc gggctgcact tccaggacta
      901 cgacgcctcc ggcagctccc agttcggctt catggtcacc tcgccggcga acgaccggac
      961 cgcgggggat cccggcacgc agcgtgtcag cgatcgcgga agcgggctgt acggcggcta
     1021 cgacgcctcg cgcggcggcg ccgagccgtg ggacgagggc cccgagccgg cctacgtcat
     1081 cgccgaggat ttctcgaacc cgacgcgtca cgcatacacc gactccgccg ccgccgcgac
     1141 gtcgatgtgc agcgggatca agacttactc cgcggcggtc aacgtcgaca ccgccggccg
     1201 gcagcagctc ccgatcgcgc gggagctgca gcagcgcggc ttcggcgtcg gtgtcgtgac
     1261 cgacgttccg atctcgcatg ccacgccggc tgccgcctac gccaacaacg tggtccgcca
     1321 cgactaccag gacctgtcgc gcgacatgct cggcctgccg tcgatcgcgc atccccgccg
     1381 ggcgcttgcc gggctcgacg tgctgatcgg ctgcggtgcc ggcgtcgagc gtgcgaccga
     1441 cgcggcgcaa ggcgacaact acgtccccgg caaccgctac ctcgcgtcgg acgatctccg
     1501 tgcgatcgac gaggccggcg gcggcgacta cgtggtggtc gttcgcgagc gcgggaccga
     1561 cgctggccgg gcgctcgagc acggagcgcg tcgagcggcc gagcgcgggc tgcgcctgtt
     1621 cggcttcttc ggcacgacct acgcgcacct accctaccgc accgccgacg gcggccacga
     1681 tccggtcgaa ggacaggatg gccaggcgga gcggtactcg gctgccgacc tcgtcgagaa
     1741 cccgacgctg gcagagatga ccgcggcggc actcgtcgtg ctgggctcgc gcgcggaagg
     1801 gttctggctg atggtcgagg ccggagacgt cgattgggcc aatcacgacg cgaacatcga
     1861 cgccgcggtc ggatcggtgc tatcgggcga cgaggcggtc cgcgtgatca ccgattgggt
     1921 cgaaacgcac agtgactgga ccgagtcgtt gttggtcgtg accgccgatc acggccacta
     1981 cccggtgttg gtcgaccccg aacgattcgc gtccttccga ccctgaggac gtcgctagtt
     2041 cgtgcgcaac gagatgttcc cgctgaacgt ttcgaccgag acctcgaagt cctcggagcc
     2101 ggtggagaag cgcagctcgg tgtagggctc gaaccgcccg gtcttgcgcg gtgacggtcc
     2161 gatcgtgttc tcgatcgatc cgttgaaggt ggagaacgag aaacgggcac gcgtggcggc
     2221 gggaacgacg atttcgaccg tcccgttgtg tgtttcgact tcgatgccgc cctgggccgt
     2281 caggctgcct tcgatcgaca cgctccccga tacggtctcg atcgacacct cttcgaacga
     2341 gcttccggtg aggatgacgc caccgctgat cgtctccacc tcgaccgcac gggtcgcccc
     2401 gacgagcgtc acgtcgccgc tgaccgattc gacctgcatc gccgcggcca ccgcctgcac
     2461 gtcgaccgtt ccggtcatcg actcgatgcg caccacgcgc ggatctccgc cgatcgacac
     2521 cgcgccgttg accgactcga cctcgatcag gccgttgacg ccgtcgatcc gtatcgaggc
     2581 gttcaggctt cgcacgccga ccgagctgcc ggtcggcagc tccacgacga gttgcgagcg
     2641 atagtcggtg tcgtcgtcag actcgtataa ccagtgctcg ggcgcctcga ccgaaatatg
     2701 gaattcgccc tggtccgcgt cgaaggcgag tccctcggca ccgggcgcca accggccgct
     2761 gacccgtacc tcgttcttcg accacgcgcg gatctcgatc gtgccgaaca tgttctcgac
     2821 cacgacctcg ggttgcttgg gggccggtag cgatcgatcg atcgtcacct gcgccgcgac
     2881 gaggccgtaa cacgataggg cgacgagact gccgacgaag ctgcggatga tggtcatcgc
     2941 gtcctcacta aatcgagtcg gtcgcgaccg gccgggcgcc gagttgggtc gcgcgctgga
     3001 gcaggttgag ctggcgttgt cgcgcggtga gcaggcgccg gcccagatcg cggttgtcgg
     3061 gatcacgcgc gagcacggtc tggatctcgt cgatcgcggc ttcgatcacc gccaagttgc
     3121 gctcgacggt ccttacggtc tcggcggaga gggcgccggg gcgctcgtcg agcagcgtcc
     3181 ggagctcgcg ctccgcctcg acgatcgctt gttcggcgag gacgagatcg ccggcgccga
     3241 cggcggtgcg gatcgcaggc gacgaggagg aggcccgcgc cggtacttgg gtcgggcgca
     3301 gctcggtccc gcgcagcagg tagccggccg ccaagccgac ggcgagcaac gacgcggcga
     3361 gcaagcccga gatccaagcc cccttcgtcg gtcgacccca cgtcgcaccg ggcccgcccg
     3421 cccgtcgtcc cgccacggcc agccggcgct cgatctcggg ccacagctcg cgtggcgggt
     3481 cgatcgctcg cggtaacgac gcggcgcgtt cgagcaggcc gcgcagcgcg cgctcggacg
     3541 agcagcagcg cgtgcagtcg gcgaggtggc gccggatcgc cgacgtctcg tcgcgagcga
     3601 gctcgccgtc caggtagtcg tcgagccggt ccaacgtcca ttgacagtcg gtcatttgtc
     3661 gagcaactcc cgcagacgct tgcgcgcccg atgcaactgc gccttggccg taccgatggc
     3721 gattccggtc atcgtcgcga tctcgtggtg gcgatagcca tgcacgtcgt gcagcacgaa
     3781 gaccgtgcgc gcaccgtcgg gcaaacaagc gatcgcgcgg tcgagatcga cgcgggattc
     3841 gcctcccgcg ccggacgccg tctcgccggt cggaacgaac tcctcgaaca acggcagcgg
     3901 ttcgccgcgc cgggaccgac gtcgacgggc actgagcgtc aggttgacgg cgacccgatt
     3961 gagccaggcg ccgaagtgcc ccggctcgcg aaaggtgtcg aggtgctgcc aggcccgcac
     4021 gaacgtctcc tgcgtcatct cttccgcctc gtgggagcgc cggccgagcc gcaggcagag
     4081 cgcgtagatg ctgccgcagt gcctgcgata cagctgctcg aaacagcgac gatcaccgcg
     4141 ctgcgcgccg agcacccact cccactcgtc ggagtcccgc tcatctttcg cctccggcaa
     4201 cgtcgctccg ccctccccat ctagctcaac gccgaagggg cggtcggggt ttactcgccc
     4261 gccggtcgat ctcgtcgagt cggaaggacc ggcgaaccac ggcggcgaca ctagccgtcg
     4321 ttctccccgg tcgtcgccgg ctggcgcatc ttgcggattc gctcgagccg ctgatcgcgc
     4381 aacttggtcg ccgaaacgcg catatcggcc acctggtcgg attcgtcgac gatctcggtg
     4441 ccgaggatcg tctcggtcag gtcttcgagg gtgacgagcc ccgccacgcc gccgtgctcg
     4501 tcggtcacga cggcgatcgg ctctcgctgc ttcagaatcc gccgcagcgc cgcgccgacg
     4561 ctggccagct ccggaatatg ggagatcggc cgcatgaatc gctcgagcgg ctgctcccga
     4621 tcgcagtgct cggcgacggc cttcaacacg tcgcgctgca agacgtagcc gacgacgtgg
     4681 tcccgatcgg gtcgaaacag cgggatccgg ctgaaaaagc gtgcctcggc ttcggcgagc
     4741 aggtcggcga tcgtggcatc ggccggaagc atgaaagtca cggttcgcgg ggtcatcacg
     4801 tcctcgacct ggatcgagtc gaatcgcagc agattggcga acatccgcga ttcctccggg
     4861 gagagaaccc cttcgcgggt cgccatcgag atcaccgtgg ccagctcgct ccgcgacagg
     4921 ctcgcgccgc cgccccgcgc cagcagccgg gtgatcactc gcgacaggaa gagcgccggg
     4981 ctcatcagct tgaccaagcc ggtcagggtc caaccgacca tcccggacag ctgggtggag
     5041 tagaccgcgc cgagggtctt cgggatgatc tccgacacga tcaggatcac gaacgtgagc
     5101 agccccgaga agacgccgac ccaagtcctg ccgaaaacct gtgccgcctg ggcaccggcg
     5161 agcgtcgcgc cgagtgtgtt ggcgatggtg ttgaggatga gaaccgcgct gatcgcgtcg
     5221 tccaacctcg actgcttcag cgtcaacagc aagccggcgc cgatgacgcc cgccgcccgt
     5281 ttctcgatca ggcgggccgt gcgcgccgac agcagcgcgg cttccaacag cgagcagaga
     5341 aaggagacgc cgagggcaaa cgcgacgaaa agaatcagga ctagcacccg gctcgatccc
     5401 cctccggcgc cacgccgacg atttcgattc tagcgcggcc ccgaccaaac ccgtcattct
     5461 cgccgtccgg gaagtcgaga cccgccgccg gctcgggcaa gtccatctgc accaagggtt
     5521 tgcccgcacc ggaatttccg ggttgtcggc acaatccgtg caaccgccag caggtgcgga
     5581 gccgatgacg cagttggtcg tcgatctcgc ttgtgccgag ggccggtcgg tcgtacaaca
     5641 cttgcaggag cggtgtatgg tgcgcatcaa cggcaaccgc agagcgtgcg ccgcaccccg
     5701 gaccgagcgg cgtcgacttc tcgccgggtg tgccgccgtc gccctgctcg gcggcgtggc
     5761 gaccgcggag gaggtccgaa tcgaaccgct tcgcctcccg gaggtgcggc tcgagaccgc
     5821 cgcgccggcg gagcggttgc gcgcggcggg ggacgagtcg ttcttgcccc gcgcgctgtc
     5881 cacgggctac ccgcgtcgcg tccagcgcgg cttctacgat acccacctgc tgccggagct
     5941 ggcccaccag atgaacggct tccgttcgct gcacgggctg cctgggtccg cggccgagat
     6001 cgaacagtcg ttgatctact cgtcggtcgg cgacagcgcc cgccgcaaga cgatcaaggg
     6061 ggcgaccaac gcgctcaagg actatctgct cgaggtcacc gagctcacca cggtgatcgg
     6121 ttcggtaccg accccgcggt tcggtggggg agaggtcgcg ggcgacgccg cgcgccgcca
     6181 gcgagcggag ctcggctttt ccttcggctt cgcccaccgc atgccgaacc tgggcgtcaa
     6241 gtaccgcatc ggctcgacgt tgacccgcct cgggctgacc ggacacggtg cggtcgatct
     6301 acgcatcgag cgggaggacg ccacgcggat gcggagctcg atcgggttca actacgaccc
     6361 gcgagacaac tggtacggaa tgaactacag cgttcgcttc tgatcctacg cccagcgctc
     6421 caggtccgcg gcctccagct tgcgaaacag gatccgaggc tggcccagcc gatgaccggg
     6481 cggcagctcg accagcgccg cgtcccaggc caagtcgctc tgttcgagtc cgagcatctt
     6541 cgccgtctcg cgtgccgaga acggcagaaa cggcgccatc agcacggtca atgcccgaac
     6601 cgtctggatg cagacgttga ccgtcgtccg gcaaccggcg gcgtcgagcg cgcgctgctt
     6661 ccacggttgc ttgcggtcga gatagcggtt cgacgagcgt gccagcgtca tcaccgcggc
     6721 caacgcggac ttgaagcgaa acgcttccag atgatccgtc gtcgcgtcgt gctgttgccg
     6781 catgagcgcg aggtgttcgc tgtcgatctc accacgatca cccgggtcgg gtactcggcc
     6841 gtcgcagtat cgctgggcga aggtcagcgt gcgattgacg aagttgccga gcgcgttgag
     6901 taactcgccg ttgttgcgcg cgacgaagtc gtcggggtcg aacgaagcgc gcgcgttctc
     6961 cggagcaatc gccgtcaggt aatagcgcag cggatcgggg tcgaaccgcg cggcgtagtt
     7021 gttgatccac aacgccgtgc cgcggctctt gctcatcttc tcaccgccca gattgagaaa
     7081 cgaattggcc accacctgcc agggcaaccg atagctgccc tccgccatca gcatcgccgg
     7141 ccagatcagc gcgtggaaca ccgtgttgtc ttcgccgatg aagtgaatga tgcggctttc
     7201 ctcgctcttc caccagcgct cgtagtccga cgcgcctccc ccgcgctcga cgcacagcgc
     7261 cgcggtgaac gagacgtagc cgatcggtgc gtcgaaccag acgtacaaca ccttgccggc
     7321 ggcgtcggga tcgtccaacg gcaccgggac gccccaggcg agatcgcgcg tcatcgcccg
     7381 ctcgggcaga ccgcgattga tctgcccgag ggtgaagttc agcacccact cccgccaagc
     7441 cggatcgccg ggaccggcct gacgcttcga ctcgagccag tcgcgcagcg gccgctcgaa
     7501 gcggccgagc gcgagatacc agtgcacggt gcttcgtcgt tccggccgcg cgccgctgat
     7561 cgtgctgacc gggtcgatca gccgcagcgg atcgatcgcg tttccgcacg actcgcattg
     7621 atcgccgttc gcctccgggt tatcgcacgg cgagccgtcg gcgcgcgcgt ggtagcaccg
     7681 accgcgcacg aaccgatcgg gaaggaactg ccgcgcctcg gggtcgtaga gctgctcggt
     7741 ctctcgcttg ttgaagtagc cccgttggtg gatcgtccga aagaacgcct ggctcatctc
     7801 gacgtgacgt tcgacgaagg cgggctggtg cgtaccaccg aagatgtcga actcgatgcc
     7861 gagactggcg aagtcttccc gctgccgggc atggtagtgc gcggtcagct cctcgacact
     7921 ctgtccttcc ttccttgccg cgatcagcga cgacacgccg ttgtcgtcgg atccgcagac
     7981 gaacagcacc tcgtcgccgc gcgcgcggcg atagcgcacg taggtgtcgg ccgggagata
     8041 ggcgccggcg acgtggccga cgtggagcaa gccgttggag tagggaagtc cggacgtgac
     8101 gagataccgc ttcgtcggca tggcggccgg gattctaaca cgcgctcgcg cgagcgaatc
     8161 ggatcggtgg ttgtcaagcg ccacgaagag ctgctatcgt gagacgaccg aggccgatca
     8221 tgctgcgaca ccccgccaac gagggacagg cgggctccca atccgccgtg ttgttcgata
     8281 aacggctgtt gccgaaacgg ttcgtcgtcg tttcgaaccg cctgccgtat cagctcgaca
     8341 tgaccgacgg tgaggtccgc ttcaagcgcg ggatcggcgg actggtcacc gcgctcgacc
     8401 cgatcctgtc gctgaccggc ggcacttggg tcggctggac gggaagctac gaccggctgc
     8461 cgccgcgggt cgaggtgcag gcggacggca cgggccagcg accctacgac ctggttcccg
     8521 ttcggctcga tccgcgacag gtcgagcagt actacctcgg ctactccaac cgctgcctgt
     8581 ggccgctgtt ccattacttc caggagcact gcgagttcaa ccgcgatcac tgggaggtct
     8641 actgcgaggt caaccgagaa ttcgccgacg ccatcgtcga ctgccacgaa gacggggaca
     8701 tgatctgggt ccacgattat cacttgatgc tgttgcc
//
LOCUS       DASPGN010000594         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_931765, whole genome shotgun sequence.
ACCESSION   DASPGN010000594 DASPGN010000000
VERSION     DASPGN010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_931765"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VD788_17210"
     CDS             <1..639
                     /locus_tag="VD788_17210"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXV78052.1"
                     /translation="LAFMLGGTLSAVLLALPATGFAHGPAARRSTVAARVAPAVAVIG
                     VGGFAVSVWLSGAITGESALPSSDDALPASDGVPARIGWVDFGQGLERAAEKDLPMVV
                     VFVTSWCGYCKKMDRSTFRDPAVVGRLSRIVPVRVDAEETTARDGYRGVELAARYRVR
                     GTPSLAVLDPRGGLIAQRSGYLAAGELLDWLDRVLPAPDTRRPEEATHVTVP"
     gene            675..1748
                     /locus_tag="VD788_17215"
     CDS             675..1748
                     /locus_tag="VD788_17215"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HXV78053.1"
                     /translation="MFHKRLFIPGPTEVRVENLAALAKPQIGHRSDEFKQLYRSVVPK
                     LRRLLRTEGKVFLFSCSSTGVWEAAIRNCVADRVLCCMQGAFSDRWQKVAEANGKNSR
                     ELRVDWGRAIRAEMVDEALSGGGFDAVTLVHNETSTGVMNHLDEIAEVMRNHPDVLFL
                     VDAVSSMAGAPIDVDAYGIDVCLAGLQKAFSLPAGLTVASVSERALERARRVTNRGYY
                     FDFLEMLKYDEREQTPSTPAIPQIHALDAQLDAILAEGPDRRFERHAMLAGIVRDWAR
                     DRFALFAEEGYESPTVSCMRNTREISVAALNQELGLQYAAISNGYGVLKEKTFRIAHM
                     GDTQEWEIRGLLATIDRIVGRRA"
     gene            1765..>2865
                     /locus_tag="VD788_17220"
     CDS             1765..>2865
                     /locus_tag="VD788_17220"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HXV78054.1"
                     /translation="MNRRSTLRLLRVASRELDRAMTQMRIPGQPRPFYIAYLVRDEEY
                     FRVQAKYGSVIQRADERRRNAFVDVRVGSYRADQVREGGLSDNDKEAESYNYVELPFG
                     DNADALAHGLWRLTDARYREAVESSLDKQSRALTYLDPGRHLPSFERRAPRVRVDWRD
                     FPAVDRDRWTEYVERTSRSIRRYDGVVDSFVEFEADHGCRVFVNSEGSRLVECTPIWS
                     VECYLWLFAADGHAFPWNIKHTVTDPDELPDEQALMLEIRRAVATLHRLAGAPTIRSF
                     CGPALLEPIPAGLLIHEAVGHRLEGNRLLSTGEGQTFKDSLGQTILPPFLTIRDDPRL
                     ARWDGHSLVGHYAFDDEGVEAQSAELVERGRLT"
BASE COUNT          436 a          971 c          994 g          464 t
ORIGIN      
        1 ctggcattca tgctgggcgg cacgctcagc gccgtgctgc tcgcgctgcc ggcgaccggg
       61 ttcgcacacg ggccggccgc tcggcgatcg accgtcgccg cgcgcgtggc gccggcggtc
      121 gccgtcatcg gggtcggcgg cttcgccgtc agcgtctggc tgagcggcgc gattaccggc
      181 gagtcggcgc tcccgtcgtc cgacgacgcg ctgcccgcca gcgacggcgt gccggcgcgc
      241 atcggttggg tcgatttcgg ccaggggctc gagcgcgcgg cggagaagga cctgccgatg
      301 gtggtggtct tcgtcacgag ctggtgcggc tactgcaaga agatggaccg cagtaccttt
      361 cgcgatccgg cggtcgtcgg tcggttgtcg cgcatcgtac cggtccgcgt cgacgccgag
      421 gagacgaccg cgcgcgacgg ctaccgcggc gtcgagctgg ccgcgcgcta ccgcgttcgc
      481 ggcacgccgt cgctcgcggt cctcgaccct cgcggcgggt tgatcgcgca gcgttcgggc
      541 tatctcgccg ccggcgagct gctcgactgg ctcgaccgcg tgctgccggc gccggacacc
      601 cgtcgacccg aggaagcgac ccacgttact gttccgtagc cgctccgctt cgtctacgaa
      661 tcagcgaggt cacgatgttc cacaagcgac tgttcatacc cggcccgacc gaggtgcgcg
      721 tcgaaaacct ggcggcactg gccaagccgc agatcggcca ccgctcggac gagttcaagc
      781 agctctaccg cagcgtcgtg ccgaagctgc gccgactgct ccggaccgag ggcaaggtct
      841 tcctgttcag ctgctcgtcg accggcgtgt gggaggcagc gatccgaaac tgcgtcgccg
      901 accgcgtcct gtgctgtatg cagggcgctt tctccgaccg ctggcagaaa gtggccgagg
      961 ccaacggcaa gaacagccgc gaactgcgcg tcgactgggg ccgtgcgatc cgcgccgaga
     1021 tggtcgacga agcgttgtcc ggcggcggat tcgacgccgt cactctcgtc cacaacgaga
     1081 cgtcgaccgg cgtgatgaac cacctcgacg agatcgccga ggtaatgcgc aaccatccgg
     1141 acgtgctgtt cctggtcgac gcggtgtcgt cgatggcggg cgctccgatc gacgtcgacg
     1201 cctacggaat cgacgtctgc ctcgccggac tgcagaaggc gttctcgttg ccggccgggt
     1261 tgacggtcgc cagcgtctcc gagcgggcgc tcgagcgcgc ccggcgggtg accaaccgcg
     1321 ggtactactt cgacttcctc gagatgctga agtacgacga gcgcgagcag acgccgtcga
     1381 cgccggccat tccgcagatt cacgcactcg acgcgcagct cgacgcgatc ctggccgagg
     1441 ggcccgaccg ccgcttcgag cggcatgcga tgctggcggg catcgttcgc gattgggcgc
     1501 gcgaccgctt cgccctgttc gccgaggagg gctacgagtc gccgaccgtc agctgcatgc
     1561 gcaacacccg cgagatctcg gtcgccgcgc tgaatcagga gctcggtctg caatacgcgg
     1621 cgatctccaa cggctacggc gtgttgaagg agaagacgtt tcgcatcgcc cacatgggtg
     1681 acacccaaga gtgggagatc aggggactgc tggccacgat cgatcgaatc gtcggccgcc
     1741 gagcgtgacg cccgggaaga tccgttgaac cgtcgctcca cgctcaggct gctgcgggtc
     1801 gcgtcgcgcg aactggaccg cgcgatgacg cagatgcgca tccccggtca acctcgaccg
     1861 ttttacatcg cctatctcgt tcgcgacgaa gagtactttc gcgtccaggc caagtacggc
     1921 tcggtgatcc agcgcgcgga cgagcggcga cgcaacgcct tcgtcgacgt gcgcgtcggc
     1981 tcgtatcgtg cggaccaggt gcgcgaaggc ggactgagcg acaacgacaa ggaggccgaa
     2041 tcgtacaact acgtcgagct gcccttcggc gacaatgccg acgcgctggc ccacggcctg
     2101 tggcggttga ccgacgctcg ctaccgcgag gccgtcgaat cgtcgctgga caagcaatcg
     2161 cgcgcgctga cctatctcga tcccggacgg catctgccgt cgttcgagcg gcgggcgccg
     2221 cgcgtccgcg tcgactggcg ggatttcccc gccgtcgacc gcgatcgctg gaccgagtac
     2281 gtcgaacgca cgtcgcggtc gatccgccgg tacgacggcg tcgtcgattc gttcgtcgag
     2341 ttcgaggccg accatggctg ccgggtcttc gtcaacagcg agggctcgcg cctggtcgag
     2401 tgcacgccga tctggtcggt cgaatgctac ctgtggctgt tcgccgcgga cgggcacgcc
     2461 tttccgtgga acatcaagca cacggtgacc gatcccgacg agctgccgga cgaacaggcg
     2521 ttgatgctcg agattcgccg ggccgtcgcc acgctgcacc ggctggcggg cgcgccgacg
     2581 atccgatcgt tctgcggtcc ggccttgctc gagccgatcc cggccgggtt gctgatccac
     2641 gaggcggtcg gtcaccggct ggagggcaac cggctgctgt cgacgggcga ggggcagacg
     2701 ttcaaggact cgctgggtca gacgatcctg ccgccgttcc tgacgatccg cgacgacccg
     2761 cggctggcac gctgggacgg ccacagcctg gtcggtcact atgcgttcga cgacgagggg
     2821 gtcgaggccc aatcggccga actggtcgag cggggccggc tgacc
//
LOCUS       DASPGN010000595         2881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_464090, whole genome shotgun sequence.
ACCESSION   DASPGN010000595 DASPGN010000000
VERSION     DASPGN010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464090"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VD788_17225"
     CDS             <1..293
                     /locus_tag="VD788_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV78055.1"
                     /translation="RLSIARALFKNAPILILDEATSHLDSESEALVQRALQNLMRDRT
                     TLVVAHRLTTVKRADRIVVMDEGRIVEIGSHDELVERDGLYRKLHDLQLQDA"
     gene            702..971
                     /locus_tag="VD788_17230"
     CDS             702..971
                     /locus_tag="VD788_17230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78056.1"
                     /translation="MNPAQSEPTELPSPPRRGFRKQGSPQPNHHLWRNGRLWWIAFTV
                     HKDDWTAERVRRSLGTDDLGEARRRRDLILRHYDRAERCRLSLRF"
     gene            1054..1536
                     /locus_tag="VD788_17235"
     CDS             1054..1536
                     /locus_tag="VD788_17235"
                     /inference="COORDINATES: protein motif:HMM:NF025227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HXV78057.1"
                     /translation="MRPIVIAGVVLAVIVGVWMFVHGAAGWYRSPSLGWLFPALATVT
                     QLGVLIWGLGKTKAQGRRYGGQVVAGLLISLVGGLLIVVVSFIWSAAFPDVAEVMEAI
                     QADQFADQGMSAEEIDEMLETTAFTRTPAFGAILGFIMTMLTGLVLSLIIAAFVRVKD
                     "
     gene            1622..2167
                     /locus_tag="VD788_17240"
     CDS             1622..2167
                     /locus_tag="VD788_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXV78058.1"
                     /translation="MPQHDRLGYYRILNIGPDAGAAEIRLAYELLKTAYREGRRNLEI
                     GKIREAYAVLGDPSARRAYDRADGRSGGGGENLLDAIRQTAARVKIVPVGLGLVAVGV
                     IVLLGLIGPDLRAQFVSFEPGDALVWSATGEALGSVVAFDQGHTFSSGAVGRAYRIEP
                     AAGGGPVWYPARDLERYCRTR"
     gene            2222..2773
                     /locus_tag="VD788_17245"
     CDS             2222..2773
                     /locus_tag="VD788_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78059.1"
                     /translation="MKPTASPATPTRLALCLMVLVPLAGCGGDSAPPTGSTDRAPAAP
                     TEGQATDATALPREPRVVDVQLQVPPVTATRPGFETVTRYTEQFYAGELEKLFERFSD
                     EMKTQLPLDRLRELHAFVAERYGREVELIDQDTQTKDEYRGFVRWARFSDHEGVIQIE
                     WILRRDDTIAGFFIRPAQPQPGG"
BASE COUNT          416 a          988 c         1027 g          450 t
ORIGIN      
        1 agcggctgtc gatcgcccgc gcgctgttca agaacgcgcc gattctgatc ctcgacgagg
       61 ccacctcgca cctcgacagc gagtcggaag cgctggtgca gcgcgcgctg cagaacctga
      121 tgcgggatcg tacgacgctg gtggtcgcgc atcggctgac cacggtcaag cgggccgatc
      181 ggatcgtggt gatggacgag ggccgcatcg tcgagatcgg ctcgcacgac gagctggtcg
      241 aacgcgacgg gctgtaccgc aagctccacg acctgcagtt gcaggacgcg tgagcgacgc
      301 cggccgagct cgcgggtcgc ggatcggccg gtaggcacgc gagccgctcg tcggcttcgc
      361 gttgctccgc gtcgtggcct gcgcggcgtg gagcgcgctc gcccgctccc cgctcgggac
      421 gaccgccgtc gacccgggcc ccgcgtcgtc gtccgggtgg accgacggtg gcacaccggc
      481 gcgcccgggc gtaatatgga ggcggattcg acatctggag tgtcccgctc ctgattgact
      541 gacgagagcg ggcccggcaa cctgggacgg acaacccaag gtgcccttct cgccgcgccg
      601 tcgcggcggg ggatgtgcta ccggcgaggt cgccggcaga acaaagtgtc cggcgcggct
      661 cgaccgcgcg ctccgggtgt cttctcgacg gaggagacgc catgaacccc gcccaatcgg
      721 aaccgaccga gctgccgagc ccgccgcgcc gcgggtttcg caagcaagga agcccccagc
      781 cgaaccatca cctgtggcgt aacggccggc tgtggtggat cgccttcacc gtccacaagg
      841 acgactggac ggcggagcgc gtgcgccgct cgctgggcac ggacgacctc ggcgaggcgc
      901 gccgccggcg cgacctgatc ctccggcact acgaccgtgc cgagcgctgt cgactctccc
      961 tgcgcttctg atcggtccgc ggcttccccc gtcgcgtctc cgggagtaga cttcggccga
     1021 ttcgaccgat ccgggagcga ggaggttgtc gccgtgagac cgatcgtgat cgctggagtg
     1081 gtgctggccg ttatcgtggg cgtatggatg ttcgtgcacg gcgcggccgg gtggtacagg
     1141 agcccgagcc tgggttggct ctttccggcg ctggcgaccg tcacgcagct cggcgtgctg
     1201 atctggggtc tgggcaagac caaggcccag ggccgccgct acggcgggca ggtcgtcgcc
     1261 ggtctgctga tcagcttggt cggcggtctg ctgatcgtcg tggtctcgtt catctggtcg
     1321 gccgcattcc cggacgtcgc ggaggtgatg gaagcgatcc aggccgatca gttcgccgat
     1381 cagggcatgt cggccgagga gatcgacgag atgctcgaga cgaccgcctt cacgcggaca
     1441 ccggcgttcg gcgcgatcct cgggttcatc atgacgatgc tgaccggcct cgtcctgtcg
     1501 ctgatcatcg cggcgttcgt ccgcgtgaaa gactgacccg gcttgacttg acggcccgtc
     1561 gaaccgaact tcgcctccgg ggcgggtccc cgcctcgatc gggggcgaag cggagggcga
     1621 cgtgccgcag cacgatcgac tcggttacta ccggattctc aacatcgggc cggatgccgg
     1681 ggcggccgag atccgcctgg cctacgagct gctcaagacg gcctaccgcg aaggacgcag
     1741 gaacctcgaa atcggcaaga tccgcgaggc ctacgccgtg ctgggcgacc cgtccgcacg
     1801 acgggcgtac gatcgggcgg acggtcggtc ggggggcggg ggcgaaaacc tgctcgacgc
     1861 gatccggcag accgcggcgc gggtcaagat cgtgccggtc ggtctggggc tggtcgccgt
     1921 cggcgtgatc gtgctgctcg gcttgatcgg tccggacctg cgcgcccagt tcgtcagctt
     1981 cgaaccgggc gacgcgctgg tctggtcggc gaccggcgag gccctcggct cggtcgtcgc
     2041 attcgaccag ggccacacct tttccagcgg agcggtcggc cgcgcgtacc gaatcgaacc
     2101 ggccgccggc ggcgggccgg tctggtaccc ggcgcgcgat ctcgagcgct actgccgcac
     2161 gcgctgagtc gggtcgcccc gggtggtggc cgatccggcc gccggttgcc ataatccccg
     2221 gatgaagccg actgcctcgc ccgcgacccc gactcgactc gccctgtgcc tgatggttct
     2281 cgtcccgctg gccggatgcg gcggcgactc cgctccgccg accgggtcga ctgaccgggc
     2341 ccccgccgcc ccgaccgaag gccaggcgac cgatgcgacc gcgcttccgc gcgagccgag
     2401 ggtcgtcgac gttcagctgc aggtgccccc ggtgaccgcg acgcgccccg ggttcgagac
     2461 cgtcacgcgc tacaccgagc agttctacgc gggtgagctc gagaagctgt tcgagcggtt
     2521 ttcggacgag atgaagaccc agcttccgct cgatcggttg cgcgagctac acgcgttcgt
     2581 cgccgagcga tacgggcgcg aggtcgagct gatcgaccag gacacgcaga ccaaggacga
     2641 gtatcgcggc ttcgtccgct gggctcgctt cagcgaccac gagggcgtga tccagatcga
     2701 gtggatcctg cgccgggacg acacgatcgc cgggttcttc atccggccgg cgcaaccgca
     2761 gccgggcggc tgagccccgg cgtcgcgcgc cgagccggcc gggatctcga caaaaccgtc
     2821 ccttccgcct accgtatctg tctgccgcag gggggtcctg atgaccgacg cgatcacgct
     2881 c
//
LOCUS       DASPGN010000596        12393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1165397, whole genome shotgun sequence.
ACCESSION   DASPGN010000596 DASPGN010000000
VERSION     DASPGN010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 12393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12393
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165397"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VD788_17250"
     CDS             complement(<1..380)
                     /locus_tag="VD788_17250"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HXV78060.1"
                     /translation="MIGESVEIVEREFGELALNMGPQHPSTHGVLRLTLYLRGETVVR
                     AIPGIGYLHRGVEKLSETLAYDQLAPVFERDDYLGPTANSLAFVLAAERHGGTDVPRR
                     GAWLRTLVAELQRVASHLVWLGTLG"
     gene            complement(377..835)
                     /locus_tag="VD788_17255"
     CDS             complement(377..835)
                     /locus_tag="VD788_17255"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXV78061.1"
                     /translation="MPAAELIAAMKVRFAAEEFALGIEIDRTRLLETLRALRDRHGYR
                     LYVSATATDRPDRIEVLHGLRNLDTCDTLWVKVGLPKQDAEVDSAAFLYAGAEWQERE
                     IFDLFGVRFRSHPDLRRILMPDEYEGHPLLKQFPIDAPWGYRPAGADRQS"
     gene            complement(822..1340)
                     /locus_tag="VD788_17260"
     CDS             complement(822..1340)
                     /locus_tag="VD788_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HXV78062.1"
                     /translation="MGQVERPRSIDEIAEEVVRFPGGGTALVRSDLLFRWAQKSSLWP
                     LTFGLACCAIEMMATFAGRFDLDRFGVILRPTPRQADVMIIAGTVTIKMAPRIRRLYD
                     QMPHPKWVISMGACANSGDHYRHVYSVVPGVDHVIPVDVYVPGCPPTPEALMEGIFTL
                     QRRIMEGERAGG"
     gene            complement(1328..1699)
                     /locus_tag="VD788_17265"
     CDS             complement(1328..1699)
                     /locus_tag="VD788_17265"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXV78063.1"
                     /translation="MERPDPYIALTVHAIVCLALGGGLLAAAAVLRNRVALSRPHKHE
                     TYECGEDPIGPAWRKTPAGFYLLALLFILFDAEAAFIFVWILGLEQLGPAAFWAMVVF
                     LAVLLIGWAYAWRRGDLTWAR"
     gene            complement(1769..2206)
                     /locus_tag="VD788_17270"
     CDS             complement(1769..2206)
                     /locus_tag="VD788_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78064.1"
                     /translation="MRQSPPIHYSVQHFVEDEARVLAATDHEDVSVRECIDVLDGRLL
                     PLPTDPLREALECKRRATDAFSSKYGPELGADHLERFRSVLDFIASHREEMAAAGLAA
                     SGETLAIREEFLDYLLNFHAGTRHGIPKTALGRFLDEWGTRWI"
     gene            complement(2365..2709)
                     /locus_tag="VD788_17275"
     CDS             complement(2365..2709)
                     /locus_tag="VD788_17275"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXV78065.1"
                     /translation="MRIRVGTTKRVCIIEVAGRLTFGDADGRLCDEVARRIDAGERRL
                     VLDASRLSLIDSAGVGEIVATYQLASARGALIRVALPSGGPIHRVFRVSGLDRALEIF
                     EHRDEAIASYLE"
     gene            complement(2710..3549)
                     /locus_tag="VD788_17280"
     CDS             complement(2710..3549)
                     /locus_tag="VD788_17280"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXV78066.1"
                     /translation="MGRAVLFGCGAASSVFLGLVWVGLPTAFPPPARGTGPTLRVAVA
                     NLAYDNDRALELTAVLRRIDPDVLVALEWTGSNLDLDTIAASERRIALDARRPAPHGV
                     LVLTRRGLAGEVALAPSPVPGPCAMPVATLRLPRGDRFVSLLGVHAPPPLPECEATNE
                     PTLRYLADQIDAGRLRRPIGAAATSDPLILAGDLNATPGSPGITRLRRGLLDAQRHRS
                     WLPAGTWLPRDGPRLSFERIDYVLLAREIPLEGAWTVDLPGSDHRALVVDIGPLPGRD
                     GER"
     gene            complement(3555..7853)
                     /locus_tag="VD788_17285"
     CDS             complement(3555..7853)
                     /locus_tag="VD788_17285"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HXV78067.1"
                     /translation="MVDRWRTHTTAASFCLLLVSTALGVSAQTSSDARPLAIRGAERS
                     APVTPFVWNGDTRDLPAPPQWRPGDPVKEIPRRAYPRPGEQPIPYYQTGLDPLVALQW
                     DYLAGRSARQFDLPSRNFPGQGYSGVNPPDTVGDVGPNHYVQMINGGGGALVAIYDKS
                     EPTPNLLSSFTLDSLGSGACASGRGDPIVLYDRLADRWMLSEFSFSGNNLCVYISQTP
                     DPTGGYYAYGFAAPSFPDYPKYGVWPTDANGGQGSYVVTANDGGPGVYALDRGAMLVG
                     AASTYQRLTIPPLSGFAFQAPTPADMDGPQPPAAATPAIIMRHRDTENHSGPAAPGDL
                     LELWEFQVDWAQPANTTLFTRPSIDVAEFDSALCGLTAFFCFPQPGSSTTLDPLREVI
                     MNRLQYMNHGDRETLVGNFVVDVDGADLGGLRWFELERSGGGWTLKQEGTYSIDDDNR
                     FMAASSMDQSGNIAIAYNITSSATFPSLRYTGRLDDDPPGVMTQPETVIHAGASPNSS
                     NRYGDYSGMNLDPADDCTFWFTGMDNLTTSWRTQIASFRFDACGCELLPDPPLIDAFA
                     NGDNRIDLGWNDSSLETVVEYVVQRSRTPGGPYQTIAVVPDDSPGVANGPGYTYADIG
                     VSGDITYYYVVVASDGEACKSDPTNEVSATATGPCTLPPIFAGLAAATTPFSAICSID
                     LSWNAAVSECGGQIEYTLWRSQDAEFDPATTLPLAEGLTGTSFLDLDTLVSDETYYYV
                     VRATDLSNGKQEQNLVRLDARPQGELTTGVWFDDAGDSAPAKMILDSPWNVDPDEGNF
                     GPNVYKTGSYADLICAALETPELRLGNGSQLTFHSKYDIEPSWDKGEVQISTDGGETW
                     ERVDVAYPGNSTNTSDNCGLPQGTYFTGSGLSWATYGADLSAYAEQFVRIRFVLSTDT
                     SVNRTGWWIDDIMITQVDVPGTCATGSTCPANPLVSVAPEGLLDVCPGATELTTQVTG
                     GSAPFSYQWTRDGVDVPGATETSWTPQDEGLHTYNCKVQSASCDEPAFAARGASLNAT
                     ATPSFGGLTAVDDAQGADCSITLDWEPAGTFCDGPIHYFVYRDTAPGVSADPAKIVAS
                     NLTATGHVDVGGLVEGQTYYYLVRALDRSTARFDGNAVEGQATPTGPGTGVYSLFFED
                     FTDANALADWPSSVSPATRTCGLWERSSSAALRPIGGAGFFATSNGSVCGNQKTATTL
                     ESPAIDLGFGGVDKVTLTLDLYYNHLDGTDTAAIDGWDGATWQPIWSSPAADVNAELT
                     FDVTSIAAGNPAFRIRFVYDDIDQWLSVDNVDLTVDVANACATSFGPSPVPDGRDQTA
                     PLRGARLSAAGDQIELTWDTASCAAADYSLLYGNLADVSSYALAGSQCGLGTSGSFVW
                     NGVPAGDLYFLLVGSDAAGSESSWGTDGLHGERNGPVASGECGATVKDLSGTCP"
     gene            complement(7853..8641)
                     /locus_tag="VD788_17290"
     CDS             complement(7853..8641)
                     /locus_tag="VD788_17290"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXV78068.1"
                     /translation="MVACGWMLGSVPASVSAADPDWRKESLDWLEPIEPGGVVRVVNP
                     LGDVHARFGGYEARVELLAVVQHDAARSAPPEVVIEHDGGGLRVAARGDAPGQRVDLV
                     LFVPRGSPFDARTERGDIELKGLEGDVTLQSIEGDVTLRSVRGRVDAKTARGTLSAAL
                     EAGVTNDPQNLTTETGEIEVHLWDDANFTVRIETSGEISTDFSLEIEHLPFAEPGKLG
                     RAVVGRGGPQLHLSSKRGRVRLLRLQRDFHPKPETGPDSTTRNR"
     gene            complement(8732..9871)
                     /locus_tag="VD788_17295"
     CDS             complement(8732..9871)
                     /locus_tag="VD788_17295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXV78069.1"
                     /translation="MTLRFCLFVDDLSGGGAERKVLILADQLVRLGHDAQVVTLTDRV
                     AYPIPAGVATRAVYPGGARNLDQFWRVGRSARDLGRIVHELEGRGGPFDLFLSNLDAT
                     NKIVSRLAVAPVYFVMRNSVEEELRRARRLGPLQYLRMRSAKMAVGGRDLITVSHGLE
                     REIVERGRLRPRSIRTILNPYDLDEIRRLSLEPVAEIPREPFLLHVGRFARQKRHDLL
                     FRALERVDPAYKLVLLAHRREKALRCAARYGVADRVIAPGFRANPYPWMRRARLTVLS
                     SDFEGFVNVLVESLACGTPAVSTRCPHGPDEILTGPLAHWLVPRRNPRALADKINEAL
                     ATRIDVGGAEILDRVRADRIAAEYVELAVRGAGDRHSRAPAGETA"
     gene            9994..12219
                     /locus_tag="VD788_17300"
     CDS             9994..12219
                     /locus_tag="VD788_17300"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXV78070.1"
                     /translation="MRHTVVVLLASLTATVAPYPADCQTEAAVGSDARRPYTVLVLSG
                     GGARGMAHIGVLEVLEQHQVPFDAIVGTSMGAVVGGLYASGVSPGEMRTQLEQIDWID
                     AFDDEPPRRVRPFRRKEDDNIPLFKLELGFNERGFQSPSGLIAGQKLNFYLRAMLLRS
                     AAVDSFDELPVPFRAIATDLDTGEMAVLDRGDLPKAIRASMAFPVMFTPVEYDGRLLI
                     DGGVVRNLAVDVALEMGAEVVIAVDVGSTIDSLKTESPSAFNVLRRTQSIQSKEGRGE
                     QLRLLREQDLLIGPQLDGIVTFADFEKIDEAVACGRAAAQEQEQRLRELADPDEFERF
                     LARQRSGASPGAIPIEAIEVRGVERVPRTRILRRILSRPGQPLDLATLQEDLKRVYLI
                     GEFETVEFHLERLGSEGYQLVIDAREKSWGPWYLRGGLGLESNFDGVGSFRANALLRR
                     AEVNRLGAEWRTLLSIGDVEMVETDLYQPLSQAGTWFVDPSLLYVADGEETLNVDGTA
                     IVLDTLRGEIELDLGVALGQWGQLRVGALTGRSHNSAIVAGVEDLDERLGAVRLRFPL
                     DTLDNAYFPRHGTQFILDQRISRQGLGADRDYERLLFEGVTAGSFGPNTLLGKLRIGT
                     DYDSDLPFYDDFRLGGFLDMSGLTRGQLLGNELGFLALVYYRKLNRSPGAFGMNYYLG
                     ASLEAGSVWEQQQAVTLSDLEPAGSIFVGAETLFGPIYLAYGQTSGTRGTFYFFLGRL
                     F"
     gene            complement(12192..>12393)
                     /locus_tag="VD788_17305"
     CDS             complement(12192..>12393)
                     /locus_tag="VD788_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78071.1"
                     /translation="PFSLELYERVGFDVLAFDRDDSDTAREMGRLLGWDGQMDLTTDL
                     HGMYTLLRKRSEGDQNNLPRKK"
BASE COUNT         1901 a         4206 c         4395 g         1891 t
ORIGIN      
        1 ccgagcgtgc cgagccagac gaggtgcgag gcgacgcgtt gcagctcggc gaccagcgtg
       61 cgcagccagg ccccgcgccg cggaacgtcg gtcccgccgt ggcgctcggc ggcgagcacg
      121 aacgccaacg agttggcggt cggcccgaga tagtcgtcac gctcgaacac cggcgcgagc
      181 tggtcgtagg ccagcgtctc ggacagcttt tccaccccgc ggtgcaggta gccgatgccg
      241 ggaatcgccc gcaccaccgt ctcgccgcgc aggtagagcg tcagccgcag caccccgtgt
      301 gtagatgggt gctgcggccc catgttcaac gccagctcgc cgaactcgcg ctcgacgatc
      361 tcgaccgact cgccgatcac gactggcggt ccgcgcccgc cggccggtaa ccccacggcg
      421 cgtcgatcgg gaactgcttg agcagcggat gtccttcgta ctcgtcgggc atcaggatgc
      481 ggcgaaggtc ggggtgcgag cgaaagcgga cgccgaacag gtcgaagatc tcgcgttcct
      541 gccactcggc gccggcgtag aggaaggcgg cgctgtcgac ctcggcgtct tgcttgggca
      601 aaccgacctt gacccacaac gtgtcgcagg tgtcgagatt gcgcagaccg tgcagcacct
      661 cgatccggtc gggtcggtcg gtcgccgtcg ccgagacgta caaccggtag ccgtgtcggt
      721 cgcgcagcgc gcgcagcgtc tcgagcagcc gcgtgcggtc gatctcgatg ccgagcgcga
      781 actcctcggc ggcgaaacgc accttcatcg cggcgatcag ctcagccgcc ggcacgctca
      841 ccctccatga tccggcgctg cagggtgaag atgccctcca tcagcgcctc gggcgtcggc
      901 ggacagccgg gaacgtagac gtcgaccggg atcacgtgat cgactcccgg gacgacggag
      961 tagacgtggc gataatggtc gcccgaattg gcgcaggcgc ccatcgagat gacccacttg
     1021 gggtgtggca tctggtcgta cagccggcgg atgcgcggcg ccatcttgat cgtgacggtt
     1081 ccggcgatga tcatcacgtc ggcctgccgc ggcgtcggcc gcaggatcac gccgaagcgg
     1141 tcgagatcga agcggccggc gaaggtcgcc atcatctcga tcgcgcaaca ggcgagcccg
     1201 aaggtcagcg gccacaggct cgacttctgc gcccagcgga acagcagatc ggagcggacg
     1261 agcgcggttc caccaccggg gaagcggacc acctcctcgg cgatctcgtc gatcgagcgc
     1321 ggccgctcta cctggcccac gtcaggtccc cccggcgcca ggcgtaggcc cacccgatca
     1381 gcagcacggc gagaaacacg accatggccc agaacgccgc cggtccgagc tgttcgagcc
     1441 cgaggatcca gacgaagatg aaggccgcct cggcatcgaa caggatgaac aacagcgcga
     1501 gcaggtagaa cccggcgggc gttttgcgcc aggccgggcc gatcggatct tcaccgcatt
     1561 cgtaggtttc gtgcttgtgc ggccggctca gcgcgacgcg attgcgcagc accgcggccg
     1621 ccgcgagcag ccccccgccg agggcgagac agacgatcgc gtgcaccgtc agggcgatgt
     1681 aggggtcggg tcgctccacg tgcgctgcgc ctccgggcga ggggcagcat cctatcagct
     1741 tagacagccc ggtaacagcc cgtcgcggtc agatccagcg ggtgccccac tcgtcgagga
     1801 accgcccgag cgcggtcttg ggaatcccgt gccgcgtgcc cgcgtggaag ttgagcaggt
     1861 agtcgagaaa ctcctcgcga atcgccagtg tctccccgct ggcggccaga cccgccgccg
     1921 ccatctcctc ccgatgcgac gcgatgaagt ccagcaccga acgaaagcgc tcgaggtggt
     1981 cggctccgag ctcgggtccg tacttgctcg agaaggcgtc ggtcgcgcgc cgcttgcact
     2041 cgagggcctc gcgcaacggg tctgtcggca gcggcagcag ccggccgtcg agcacgtcga
     2101 tgcactcgcg gacgctgacg tcctcgtggt cggtcgccgc cagcacacgc gcttcgtcct
     2161 cgacgaagtg ctgcacgctg taatgaatgg ggggtgactg cctcatgccg ccctcctccg
     2221 gatggtcgag cccccgaccg ggcgcggtgg acggggcgcc tggacccatg cctatcgtat
     2281 tctacgcccg gtgccggggt ccgcgcccgt gggaaaggtg ccggtcccgg tccggttacc
     2341 gcggcccggg ccactcgacc ctccctactc gaggtagctc gcgatcgcct cgtcgcgatg
     2401 ctcgaagatc tcgagcgccc ggtcgagacc cgatacgcgg aaaacgcggt ggatcgggcc
     2461 gccggacggc aacgcgaccc ggatcagcgc gccacgcgcg gaggcgagct ggtaggtggc
     2521 gacgatctcg cccactcccg cgctgtcgat gaggctcagc cggctcgcgt ccagcaccaa
     2581 gcgccgctcg ccggcgtcga tccggcgcgc gacctcgtcg cacaagcgcc cgtcggcatc
     2641 gccgaaggtc aaccgacccg caacctcaat gatgcagacc cgtttcgtcg tccccacccg
     2701 aattcgcatt cagcgttctc cgtcgcgacc agggagcgga ccgatatcga cgacgagcgc
     2761 acggtggtcc gagccgggga gatcgaccgt ccacgccccc tcgagcggga tctcgcgcgc
     2821 cagcagcacg tagtcgatcc gctcgaagct caaccgcggt ccgtcgcgtg gcagccacgt
     2881 cccggcgggc aaccacgatc ggtggcgctg cgcgtcgagc aacccccgtc gtaagcgggt
     2941 gatgccgggc gagccggggg tggcgttgag gtcgccggcg agaatcagcg ggtcggaggt
     3001 ggcggccgcg ccgatcgggc gccgcagccg gcccgcgtcg atctggtcgg cgagatagcg
     3061 cagcgtcggc tcgttcgtcg cctcgcattc cggaagcggc ggcggggcat ggacgcccag
     3121 caggctgacg aaccggtccc cgcgcggcag ccgcagcgtg gcgaccggca tcgcgcacgg
     3181 accggggacc gggctcggcg cgagcgcgac ctcgccggcc agcccacgcc gggtcaggac
     3241 cagcacgccg tgcggggccg gacgccgtgc gtcgagcgcg atccgacgtt cactggccgc
     3301 gatcgtgtcg aggtcgagat tggagccggt ccactcgagg gcgacgagca cgtccggatc
     3361 gatccggcgg agcaccgccg tcagctcgag cgcgcggtcg ttgtcgtagg ccaggttggc
     3421 gacggcgacg cgcagcgtcg gcccggtgcc tcgcgccggc ggaggaaacg cggtcggcag
     3481 tccgacccac accaaaccga ggaagaccga gctggcagcg ccgcagccga acagcaccgc
     3541 ccgccccatc gtgctcaggg gcaggtcccc gacagatcct tcacggtcgc gccgcactcg
     3601 cccgaggcga cgggtccgtt gcgctcgccg tggagaccgt cggtacccca gctgctctcg
     3661 gaaccggcgg cgtccgagcc gaccagcaag aagtagaggt ctcccgccgg gacgccgttc
     3721 cagacgaagc tgcccgaggt ccccagcccg cactggctgc cggccagcgc gtagctcgac
     3781 acgtcggcca ggttgccgta gagcaggctg tagtccgccg cggcacagct cgcggtgtcc
     3841 cacgtcagtt cgatctgatc gccggcggcg ctcagccggg ccccgcgcag cggcgccgtc
     3901 tggtcgcggc cgtccggcac gggagacggc ccgaagctcg tcgcgcaggc gttggccacg
     3961 tcgaccgtga ggtcgacgtt gtcgacgctg agccactggt cgatgtcgtc gtagacgaag
     4021 cggatgcgga acgcggggtt gcccgcggcg atcgacgtca cgtcgaaggt cagctcggcg
     4081 ttgacgtccg cggccggact gctccagatc ggctgccacg tcgcgccgtc ccagccgtcg
     4141 atcgccgcgg tgtcggttcc gtcgaggtgg ttgtagtaga ggtcgagcgt gagcgtcacc
     4201 ttgtcgacgc cgccgaagcc gaggtcgatc gccggtgatt cgagcgtggt cgcggtcttt
     4261 tggttgccgc agacgctgcc gttcgaggtg gcaaagaacc cggcgccgcc gatcggccgc
     4321 agcgcggcac tcgacgagcg ttcccacaat ccgcaggtgc gcgtcgcggg gctgacgctc
     4381 gacggccagt cggccagcgc gttcgcgtcg gtgaagtcct cgaagaacag gctgtagacg
     4441 ccggttccgg ggccggtcgg cgtcgcctgg ccttcgacgg cgttgccgtc gaaacgagcg
     4501 gtcgagcgat cgagcgcgcg aaccaggtag tagtaggttt ggccctcgac cagtccgccg
     4561 acgtcgacgt ggccggtcgc cgtcaggttg gacgcgacga ttttggccgg atccgcgctg
     4621 acgccgggcg ccgtgtcgcg atagacgaag tagtggatcg gcccgtcgca gaaggtgccg
     4681 gcgggctccc agtcgagcgt gatcgagcag tctgcgccct gcgcgtcatc gaccgcggtc
     4741 agcccgccga acgacggcgt cgccgtcgcg ttgagcgacg ctccgcgcgc ggcgaacgcg
     4801 ggttcgtcgc agctcgccga ctggaccttg cagttgtagg tgtgcaggcc ctcgtcctgc
     4861 ggcgtccagc tggtctcggt cgctcccggg acatcgacgc cgtctctcgt ccactggtag
     4921 ctgaacggcg cgctgccgcc ggtgacctgc gtggtcagct ccgtcgcgcc cggacagacg
     4981 tcgagcaggc cctcgggggc gacgctgacg agcgggttcg cggggcaggt gctgccggtg
     5041 gcacacgtcc ccggcacgtc gacctgggtg atcatgatgt cgtcgatcca ccagccggtg
     5101 cggttgaccg acgtatcggt cgacagcacg aagcggatgc ggacgaattg ctcggcgtag
     5161 gccgacaggt cggcgccgta ggtcgcccag ctcaacccgc tgccggtgaa gtacgtaccc
     5221 tggggcagac cacagttgtc ggacgtgttg gtcgagttgc cggggtaggc gacgtcgacg
     5281 cgctcccagg tctcgccgcc atcggtcgag atctgcacct cgcccttgtc ccagctcggc
     5341 tcgatgtcgt acttcgagtg gaacgtgagc tggctgccgt tgcccagtcg aagctcgggt
     5401 gtttcgaggg cggcgcagat caggtcggcg taggagcccg tcttgtagac gttcggcccg
     5461 aagttgcctt cgtccggatc gacgttccac ggggagtcga ggatcatctt ggccggcgcg
     5521 ctgtcgccgg cgtcgtcgaa ccagacgccc gtcgtcagtt cgccctgggg acgggcgtcg
     5581 aggcgcacga gattctgttc ctgcttgccg ttcgacagat cggtggcgcg gacgacgtag
     5641 tagtaggtct cgtcgctgac cagcgtgtcg aggtcgagga acgaggtgcc ggtcaggcct
     5701 tcggccagcg gaagggtggt ggcggggtcg aactcggcgt cctgcgaccg ccacaacgtg
     5761 tactcgatct gcccgccgca ctcggacacc gcggcattcc agctcagatc gatcgagcag
     5821 atcgcgctga acggcgtggt cgcggcggcc agcccggcga agatcggcgg cagggtgcag
     5881 gggccggtcg ccgtcgccga cacctcgttc gtcgggtccg acttgcacgc ctctccgtcg
     5941 ctggcgacga cgacgtagta gtaggtgatg tcgccgctga cgccgatgtc ggcataggtg
     6001 tagcccggtc cgttcgcgac gccggggctg tcgtcgggga cgacggcgat cgtctgatag
     6061 ggcccgccgg gggtacgcga gcgctggacg acgtactcga ccacggtctc gagcgacgag
     6121 tcgttccagc cgaggtcgat ccggttgtcg ccgttggcga acgcgtcgat cagcggcgga
     6181 tccgggagca gctcgcagcc gcaggcgtcg aagcggaacg aggcgatctg cgtccgccaa
     6241 ctggtcgtca ggttgtccat gccggtgaac cagaacgtgc aatcgtcggc cgggtcgagg
     6301 ttcattccgg agtagtcgcc gtaacggttg ctcgagttgg gcgaggcgcc ggcgtggatc
     6361 accgtctccg gctgggtcat cacgcccggc ggatcgtcgt ccaagcggcc ggtgtagcgc
     6421 aggctgggga aggtcgccga ggaggtgatg ttgtaggcga tcgcgatatt gcccgactgg
     6481 tccatcgagc tggccgccat gaaccggttg tcgtcgtcga tcgagtaggt gccctcctgc
     6541 ttcagtgtcc agccgccgcc gctgcgctcc agctcgaacc agcgcaggcc gcccagatcc
     6601 gccccgtcga cgtcgacgac gaagttgccg accagcgtct cgcggtcgcc gtggttcatg
     6661 tactgcaggc ggttcatgat cacttcgcgg agcggatcga gcgtggtcga cgagcccggt
     6721 tgcgggaagc agaagaacgc ggtcagcccg cacagcgccg aatcgaactc ggccacgtcg
     6781 atgctgggcc gcgtgaacag cgtggtgttc gccggctggg cccagtcgac ctggaactcc
     6841 caaagctcga gcaggtcgcc cggggcggcc ggaccgctgt ggttttccgt atcgcggtga
     6901 cgcatgatga tcgccggcgt cgccgctgcg ggcggctgcg ggccgtccat gtcggccggc
     6961 gtcggcgcct ggaacgcgaa accggagagc ggcgggatgg tcaggcgctg gtaggtgctg
     7021 gccgcgccga ccagcatcgc gccgcgatcg agcgcgtaga cgccggggcc gccgtcgttg
     7081 gccgtgacga cgtaggagcc ctggccgccg ttggcgtcgg tcggccacac gccgtacttc
     7141 gggtagtcgg gaaacgacgg cgcggcgaac ccgtaggcgt agtagccgcc ggtcgggtcg
     7201 ggcgtctgcg agatatagac gcacaggttg ttgcccgaga acgagaactc gctgagcatc
     7261 cagcgatcgg ccagccggtc gtagagcacg atcgggtcgc cgcgcccgct ggcgcacgcg
     7321 ccgctgccga gactgtccag cgtgaagctc gacagcagat tgggcgtcgg ctcgctcttg
     7381 tcgtagatcg cgaccagcgc ccccccgccg ccgttgatca tctgcacgta gtggttcggc
     7441 ccgacgtcgc cgacggtgtc gggcggattg acgccggagt agccctggcc ggggaagttg
     7501 cgcgacggca ggtcgaactg gcgcgccgaa cgaccggcga ggtagtccca ctgcaacgcg
     7561 accagcggat cgagcccggt ctggtagtac gggatcggct gttcgccggg ccgcggatag
     7621 gcccgccgcg gaatctcctt caccggatcg cccggccgcc attgcggcgg cgccggcaga
     7681 tcgcgggtgt cgccgttcca gacgaacggg gtgacgggcg cgctgcgctc ggcaccgcga
     7741 atcgcgagcg ggcgggcgtc cgatgacgtc tgcgccgaca cgccgagcgc cgtcgagacg
     7801 aggagcaagc agaacgatgc ggcggtggtg tgggttcgcc agcggtctac catcagcggt
     7861 tcctcgtcgt ggagtctggt ccggtttccg gtttcggatg gaaatcgcgc tgcaagcgca
     7921 gcagacgcac ccggccacgt ttgctcgaca ggtggagctg cggcccgccg cgtccgacga
     7981 cggcccgccc gagcttgccg ggttcggcga acggcaggtg ttcgatctcg agcgaaaaat
     8041 cggtgctgat ctcgccggag gtctcgatgc gcacggtgaa gttcgcatcg tcccacagat
     8101 gcacctcgat ctcgccggtc tccgtcgtca gattctgcgg gtcgttcgtc acaccggcct
     8161 cgagcgccgc cgacagcgtg ccgcgcgcgg tcttggcgtc gacccggccg cggaccgagc
     8221 ggagcgtcac gtcgccctcg atcgactgga gcgtcacgtc gccctcgagc cccttgagct
     8281 cgatgtcgcc acgttccgtg cgcgcgtcga acggcgagcc tcgaggaacg aacagcacca
     8341 ggtcgacgcg ttggccgggc gcgtctccgc gcgccgcgac ccgcaacccg ccgccgtcat
     8401 gctcgatcac gacctcgggc ggcgccgatc gcgccgcgtc gtgctggacc accgccagca
     8461 gctcgactcg ggcttcgtag ccgccgaaac gggcgtgcac gtcacccagc ggattgacca
     8521 cgcgaaccac ccccccgggc tcgatcggtt cgagccagtc gagggactcc ttgcgccaat
     8581 ccgggtcggc cgccgagacc gatgccggca cggagccgag catccatcca caggcgacca
     8641 cgcagagacc ccccctcgcg atcgggcgat gagcccgccg acaggtcacg cttgtcaccc
     8701 agaggaacca tacggcggtc gggcggggca ctcaagcggt ttcgccggcg ggggcccggg
     8761 aatgtcgatc tccggcacca cgcaccgcca gttcgacgta ctcggcggcg atgcgatccg
     8821 ctcgcactcg gtccaggatc tcggccccgc ccacgtcgat ccgcgtcgcc agcgcctcgt
     8881 tgatcttgtc ggccagcgcg cgcgggttcc tgcgcggcac cagccagtgg gccaacggcc
     8941 cggtgaggat ctcgtcgggg ccatgcgggc atcgcgtgct gaccgccggg gtgccgcagg
     9001 ccagcgactc gacgaggacg ttgacgaacc cctcgaagtc ggagctcagg acggtgagcc
     9061 gcgcccggcg catccacggg tacgggttcg cgcggaagcc gggtgcgatc acccggtcgg
     9121 ccacgccgta gcgcgcggcg cagcgcagcg ccttctcccg ccggtgggcg agcagcacca
     9181 gcttgtacgc cgggtcgacg cgctcgagcg cccggaacag gaggtcgtgt cgcttctggc
     9241 gcgcgaagcg gccgacgtgc agcagaaacg gctcgcgcgg gatctccgcc accggctcga
     9301 gcgacaggcg gcggatctcg tcgaggtcgt acgggttgag gatcgtgcga atcgagcgcg
     9361 gccgcaggcg tccccgctcg acgatctctc gctcgaggcc gtgcgagacg gtgatcagat
     9421 cgcgcccgcc gactgccatc ttcgccgacc gcatccgcaa gtactgcagc ggcccgagcc
     9481 ggcgggcccg gcgtagctcc tcctcgacgg agttgcgcat gacgaaatag accggggcga
     9541 cggcgagcct cgagacgatc ttgttggtcg cgtccaggtt ggacaggaac aggtcgaacg
     9601 gcccaccgcg gccctcgagc tcgtgcacga ttcggccgag gtcgcgcgcc gagcgaccga
     9661 cgcgccagaa ctggtcgagg ttccgggctc cgccgggata gacggcgcgg gtcgcgaccc
     9721 cggccgggat cgggtaggcc acgcgatcgg tcagcgtgac gacctgtgcg tcgtgcccca
     9781 gccgcacgag ctgatcggcc aggatcagca ccttgcgctc cgcgccccca cccgacaggt
     9841 cgtcgacgaa caggcagaag cgcagcgtca tcgagggcag ctcacgcgcg aggcacctcg
     9901 cggtcggcgg aagcgccgat tgtaatccac cgccgggcgc tcgtcgggct agaatcgcgc
     9961 tcggcattgg cgccgcacaa gcgggaggcc gggttgcgtc acaccgtcgt cgtcttgctt
    10021 gcctcgctga cggcaacggt cgcgccctac ccggcggatt gccagaccga ggccgctgtc
    10081 ggtagcgatg ctcgccggcc gtacaccgtg ctcgtgctgt cgggtggcgg ggcgcgcggg
    10141 atggcccaca tcggcgtgct cgaggtgctc gagcaacacc aggtcccgtt cgacgcgatc
    10201 gtcggtacca gcatgggagc ggtcgtcggc gggctgtacg cctccggtgt ctcgccgggc
    10261 gagatgcgca cgcagctcga gcagatcgat tggatcgacg cgttcgacga cgaaccgccg
    10321 cggcgagtgc ggcccttccg ccgcaaggaa gacgacaaca tcccgctgtt caagctcgag
    10381 ctggggttca acgagcgcgg ctttcagtcg ccgtccggtc tgatcgccgg acagaagctc
    10441 aacttctatc tgcgcgcgat gctgctacgt tcggccgcgg tcgattcgtt cgacgaattg
    10501 ccggtgccgt tccgcgcgat cgcgaccgac ctcgatacgg gcgagatggc ggtgctcgat
    10561 cgcggggacc tgcccaaggc gatccgggcc agcatggcgt tccccgtgat gttcaccccg
    10621 gtcgagtacg acggccggct gttgatcgac gggggcgtcg tgcgcaacct ggcggtcgac
    10681 gtcgcgctcg agatgggggc ggaggtcgtc atcgcggtcg acgtcggctc gacgatcgac
    10741 agcctgaaga ccgagtcgcc gtcggcattc aacgtgctgc gtcggacgca gagcatccaa
    10801 tcgaaggagg ggcgggggga gcagctccga ctgctgcgcg agcaggatct gctgatcggc
    10861 ccgcagctcg acgggatcgt gaccttcgcc gacttcgaga agatcgacga ggcggtcgcg
    10921 tgcggccgcg cggccgcgca agaacaggaa cagcggttgc gcgagctggc cgacccggac
    10981 gagttcgagc ggttcctcgc ccggcaacgc agcggggcct cgcccggggc gatcccgatc
    11041 gaggcgatcg aggtgcgagg cgtcgagcgc gtcccgcgaa cgaggatcct caggcgcatc
    11101 ctcagccgcc ccgggcagcc gctcgatctc gccacgctgc aggaggacct caagcgggtc
    11161 tacctgatcg gcgagttcga gacggtcgaa ttccacctcg agcggctcgg cagcgaaggc
    11221 taccagctcg tgatcgacgc gcgggagaag agctggggcc cgtggtacct gcgcggcggg
    11281 ctcggcttgg agtccaattt cgacggcgtc ggcagctttc gcgccaacgc cctgttgcga
    11341 cgcgcggagg tcaaccgcct gggagccgag tggcgcaccc tcctttcgat cggcgacgtc
    11401 gagatggtcg agaccgacct gtatcagccg ctgtcccagg ccggaacgtg gttcgtcgat
    11461 ccgtccctgc tgtacgtcgc cgacggcgag gagacgttga acgtcgacgg caccgcgatc
    11521 gtgctcgata ccctgcgagg ggagatcgag ctcgacctcg gcgtcgcgct cggacaatgg
    11581 ggacagctac gcgtcggcgc gctgaccgga cgctcgcaca attcggcgat cgtcgccggc
    11641 gtcgaggatc tcgacgaacg actcggggcg gtgcgcctgc gctttccgct cgacacgctg
    11701 gacaacgcct atttcccacg ccacggaacc cagttcatcc tcgaccagcg gatctcgcgc
    11761 caggggctcg gcgccgatcg cgactacgag cgcctgctgt tcgagggtgt gacggcgggg
    11821 tcgttcgggc cgaacacgct gttgggcaag ctgcgcatcg ggaccgacta cgattccgac
    11881 ctgccgttct acgacgattt ccggctcggc ggctttctcg acatgagcgg gctgacgcgc
    11941 gggcagctgc tcgggaacga gctgggcttt ctggcgctcg tctactaccg caagctgaat
    12001 cggtcgccgg gggcgttcgg catgaactac tacctcgggg cttcgctcga ggccggcagc
    12061 gtctgggagc agcaacaggc ggtcaccttg agcgacctcg agccggccgg gtcgatcttc
    12121 gtcggagcgg agacgttgtt cgggccgatc tacctggcgt acggccagac ctcgggaacg
    12181 cgcggcacgt tctacttctt cctcggcagg ttgttctgat cgccctccga tcgcttccgc
    12241 agcagcgtgt acataccgtg cagatcggtt gtcaggtcca tctgcccgtc ccaaccgagc
    12301 agccgcccca tctcccgcgc cgtgtccgag tcgtcgcggt cgaaggccag cacgtcgaag
    12361 ccgactcgct cgtacagctc gagcgagaac ggc
//
LOCUS       DASPGN010000597        17011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_229769, whole genome shotgun sequence.
ACCESSION   DASPGN010000597 DASPGN010000000
VERSION     DASPGN010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 17011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17011
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229769"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..2973
                     /locus_tag="VD788_17310"
     CDS             292..2973
                     /locus_tag="VD788_17310"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV78072.1"
                     /translation="MLHAGDTLLHYRVVERIGEGGMGVVWRATDTRLGRDVAIKLLPE
                     SVTADDRRLVRFEREARALAALKHPNIVTIYAVEQTGETWLLVMEYVDGTPLSAQIPD
                     RGLPLGRLLELVIAVSDAVAEAHRHGVAHRDLKPDNIVVGADGRPRVLDFGLAKLIDV
                     GSAGADSFDGTVTRGPGQTVAGEWLGTPAYMSPEQAEGRPADARSDVFALGVVLYEMA
                     TGQRPFRADNPARLVTSILTERPRSLAELRPDLPAAFGRIVEHCLDKDPDRRFQTAGE
                     LHDRLVALHDGPRPAHRARRAASSLAIVAAAAAVVAALWFGLARDRTAPAPAAPAPPT
                     AHVLSQLTFDPATEQWAAFSPDGNALVYAKETAGFSKLFLRDVATGRTRQLTAGDRDD
                     IQPAWSPDGAIVVFVRASNERGKLEPSEALAGVYDDGALWAVDVSDGRETLVVDDAFN
                     PAFSPDGSRIAVDASWAGPRRIWTTDARGRNPQQVTTGESEAVQHTQPAWSPDGARIV
                     FQQGEKTKLDLRAVDATGGESIAVTDDLYSDLDPVWSADGGHIYFSSYRGGGLNIWRV
                     PVAADGRPVGAPQQLTTGAGQDIQASLAPDGDRLVFTVLSQNADLWRLPVDPGTGAVT
                     GEAEPIVVSTREDSRGAWSPDGRRLAFNSDRDGTMNVWIHSLADGATERLTSGPGGDY
                     QPRWSHDGRSIAFFSARSANAEIWLVDVETRVIRQLTDHPALDTNPFFSPDDRSIAFQ
                     SDRDGRKEVWLVAVDGGEPRRLSTVGAADHFMRWSDDGRLVYFRSSARPENGICRVPV
                     SGGEADCLGVPLGWHMSFSPDRTRVMDVVGHKGLWVYPIDGGSGVEVHRSAEPDVRVD
                     YPEWSPDGRWVVYDRVVPRGGDVWLLDGLTAGGPRAR"
     gene            3080..3655
                     /locus_tag="VD788_17315"
     CDS             3080..3655
                     /locus_tag="VD788_17315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXV78073.1"
                     /translation="MPRSHPSVRSGPDEAESADRRDLDTDRTTDATTSLLRQGYRFAL
                     SLTHDRARAEDLLHDAWIAVRKANGPRTARYLITAIRSCFVDRCRRALIAPMESLDTG
                     PQRAANAEDLSWDDPYDAVAMKSALERALGLLRPEERAVLLLSAVEGYTAREIGELLD
                     APRGTVLSLMHRTRAKMRRWLADPTDSEERR"
     gene            3652..4245
                     /locus_tag="VD788_17320"
     CDS             3652..4245
                     /locus_tag="VD788_17320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXV78074.1"
                     /translation="MTNDDRTLGTLRDAVRDYYDGQAVDDAVLRRLMAGRERDERPWR
                     WAIAGALAAMLVLAATGVGLKGSAWLPARGDAEATPALPRLVAVQIRADWCARTPEVG
                     PVFARLVSQYGGEPILFVTVDISDDARREQAALLTANLGIPEALEAPFESGMIKLLDR
                     DRRRLLAAVTDPAQLEQFETRIAEALSGAKGDARGGA"
     gene            4416..5663
                     /locus_tag="VD788_17325"
     CDS             4416..5663
                     /locus_tag="VD788_17325"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXV78075.1"
                     /translation="MDVARPRKKRYLARGLTVGAGVGILGAVTVGLYGISSPPPTVVR
                     SAVWIDTVRRGPMVIEVRGAGTLAPEQVRWIVADTAGRVEARLLDAGAAVAADTVLVE
                     LRNPELELAAQQALLELRAAEAETQSLRVALEHELLDQEALAATVEADWRQAQLEVES
                     NEELAKDGLVPQLTLKIARVRADELALRRGLEHRRLASAERATAARLAAQQARVDQLA
                     AVAALRRREVAGLEVRAGTAGVLQDIPVEPGQSVAAGAVIARVAEPERLEARLLIPQS
                     QASEVQLGQPATIDTRNGSVSGSVVRIDPAVRNGTVTVDVTLEGELPRGARPDLTVNG
                     TITIEHLTDVLHVGRPAFAPAGEPVGLFRLVPETNDAERVEVVFGRSSVRLAEVVSGL
                     AEGDQVILSDVPGLGDAKTVRLQ"
     gene            5680..8160
                     /locus_tag="VD788_17330"
     CDS             5680..8160
                     /locus_tag="VD788_17330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXV78076.1"
                     /translation="MTSFLFDLRFAARSLARNPGFAGIAILTLGLGIGANTAIFSVVE
                     AVLLRPLSYADPERIVRVYTEFPTMSLPRFWVSPPEYRELQRDARSFEALGAWAEAGT
                     NLGGDDGPLRVFSVYATHELFDVLGVPPALGRVFGPEEDVRDGPLAVILSDGLWRRAY
                     GARSDIVGAEVLADGIPATVAGVMPPGFEFPPGSATPAEAWLPLQLPPEPENRSNHSL
                     HVIGRLAPNVTIEQARDEADRLIDAWVGGAGDAPQLYNPSHGHRLNENHPLLLHRYHD
                     EVVRAVRPAVLMLMGAVGFVLLIACVNVGNLLLARAEVRQREIAMRAALGAGTARLLA
                     QFLAEGVLLAAAGGGVGILLANWGLAGLLAADPSGLPRAAEVTIDGRVLLFTLGVSLL
                     AAVLFALAPLTHVRGMRLASRLHEGGHRVAGTLSGQRLRRALVVAQVALTVVLVTGTG
                     LMVRAFWKLQDVDPGFRPSGVLTAQVELVGPAFETAEARHAFWVSLQERLAGVPGVAR
                     VTIASGLYPERPLNANDTDFEGYDPTSDEDPHENVDFYQNVGRDFFDTAGIAIVEGRD
                     FEEADRAPGAAPVVIANQALARRFFPGATALGQRVRVGGGENPWMTIVGIAADVRNYG
                     MDTPARAELYFDLGQALQYGFQPSLLNLLVRTDGDPRPLAPSIRAAVREIDPQVPLFR
                     VRTMDEVVAAAMSRPRFLALLLTVFSALAIALAAVGIYGVVSYQVAQRRQEIGVRVAL
                     GADRPVILRMVMVAGLRLVVLGIVLGVGGAIALTRVMQGLLYGVSPLDAPSFLGATVL
                     LLAVAAAACWLPALAATRVDPMVALRTE"
     gene            8177..8881
                     /locus_tag="VD788_17335"
     CDS             8177..8881
                     /locus_tag="VD788_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXV78077.1"
                     /translation="MSTPNQENRPLIRLEALTKVFIADEIETHALSNVQLTVTRGEYV
                     SIAGPSGCGKTTLLSILGLLDAPSHGRYWLDGSDVAALGSAARAKVRNRQIGFIFQSF
                     NLIGELTVAENVELPLTYRGLSASERGKRVAQALERVAMGHRGRHYPSQLSGGQQQRV
                     AVARALVGEPSILLADEPTGNLDSHNGEAVMTLLDDLHRAGSTICMVTHDPRFARHAQ
                     RTVHLFDGQIVEERAA"
     gene            8892..10289
                     /locus_tag="VD788_17340"
     CDS             8892..10289
                     /locus_tag="VD788_17340"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXV78078.1"
                     /translation="MAAPSEVAARAARVLVADDRPDVREALRLLLRPEGFEVEVVGSP
                     AGVLAALEARDFDVVLIDLNYTRDTTSGEEGLDLLRRIRALDAHLPVVVMTAWGSVEL
                     AVEAMRRGARDFVEKPWDNARLLATLRTQTDLGRALRSEERFAVASRLATGDGDAPLV
                     AESAAMTPVLELVERIGPSDANVLITGENGTGKGRIARALHAVSARSLRPFVVVNVVG
                     LAEGVFESELFGHVRGAFTDARADRIGRLEAADGGTLFLDEIGHLPAALQAKLLRVLE
                     RGEFERVGSSATRRLDLRIVSATNAELDVEVREGRFRQDLLFRLNTIEIRLPPLRARR
                     EDVVPLANSFLARAARRYGRDVRGFEPEAYEAMVAHPWPGNVRELEHAVERAVLLASG
                     SAVREPDLRLRPPATASGTTPVAGGLAELSLEEVERLLIRKALDRHGGNVKQAADALG
                     LSRSALYRRLQRHGL"
     gene            10324..11610
                     /locus_tag="VD788_17345"
     CDS             10324..11610
                     /locus_tag="VD788_17345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV78079.1"
                     /translation="MRDTALLGGSGAVLATIVLLWTADLAPRTRWTVVLAAVVPWLVA
                     AWTVEDRAARPLRALANLLEALRAGDTSFRLRSERPGDALDEVVREANALRDMLRGER
                     LATREATALLRAVTEEIDVALFVLDAERRVRFANRRGARLLGKSPEALAGMGAAEAGL
                     RAAVTAGGAAVRPIDFPGDSGRWQVRRVPLREGGLPRELVTLSDVGPTLREEERQAWQ
                     RLMRVLGHEVRNSLAPIRSIAEGLRGRLARREDAAETRRDLLDGLAIITDRSASLGRF
                     LEGYARLARLPRPVLAPVEVGLFVRDAARLETRIPVQVDEGAPVTVRADRAQLEQVLI
                     NLLRNAADASIETGGGVRLGWATVRGALEIHVEDDGPGLGAAENLFVPFYTTKPDGTG
                     IGLVLSRQIVEAHGGTLTLENRRAVAGCLATLRLPL"
     gene            11704..13170
                     /locus_tag="VD788_17350"
     CDS             11704..13170
                     /locus_tag="VD788_17350"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXV78080.1"
                     /translation="MILIPIGHEEEARRLPWVTFVILGLCGLAFLLTGFGGTRSEWDT
                     LESHERAIEYWLEHPYLRLDPEIERAMHGMATQEMRDTFREAIRTNVDVPAAETVSVE
                     QLELDRLCEHAKNASPDHPFYRFGLVPSCIGPVTLITHMFLHAGWLHLLGNLFILYLC
                     APFVEDVWGRPLFAGFYAVSGIVAALTFALPHSSLTEPLVGASGAIAGVMGAFAIRYA
                     RTRVQFFYAIGLMTRGTFWAPAWFMLGLWFAQQVFLAMLTSNTDGPGDKVAYLAHAGG
                     FVFGAAVAFGMNRYRVEERWLAGAIEARANTVVMRNETVDAALGAAAAGDPTRAWELL
                     TGELRRNPTNVDAAMALWSVSQDAGRQSEAAPAFARMIEHQVRTGNLDGALAHWEELR
                     AAVPTTMLDVRTLVRIAGAYVEGGHRKEAADALRRALLGAGGAPNPTILLRIAETATG
                     IDPALARAAATVALGRSDLDEASRGRARSLAEPSATRT"
     gene            complement(13194..13721)
                     /locus_tag="VD788_17355"
     CDS             complement(13194..13721)
                     /locus_tag="VD788_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78081.1"
                     /translation="MFGLVFTLLLASQPCLITAEETARQLALDWEAFDQGPKGFRAFV
                     SEQRYCPRETGELIESYLDAHPGLTVQQRYVSEFHAGQQFAGANDTSRALAHFYRGFN
                     PHEDPAGRGKWNAYVRATIAFLEKDRETLEASYRVLDRQRDNPMNAINLRLVEGFRRA
                     FDGTYGEAVASAFEE"
     gene            complement(13726..14388)
                     /locus_tag="VD788_17360"
     CDS             complement(13726..14388)
                     /locus_tag="VD788_17360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXV78082.1"
                     /translation="MFKRALIVIVLSVLPVGAGVSAEDKAQEDIVFQQIDEQISKAGV
                     DKQSANWRTSLPLPKMVTFPEGKTYFASMETTKGPIEIELMPTVAPMHVTSFIYLTRL
                     GFYDGLKFHRVIPKFMAQGGCPLGSGTGGPGYEFDGEFKPGVKHDRGGLLSMANAGPG
                     TDGSQFFLTFVPTPWLDGKHTIYGEVTDGMETLKALEAAGSSSGRPNELLEIKKVTIG
                     VR"
     gene            complement(14466..>17011)
                     /locus_tag="VD788_17365"
     CDS             complement(14466..>17011)
                     /locus_tag="VD788_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78083.1"
                     /translation="VREDYSATGDAWGHFPHDHARSRAYRWNEDALGGFCNRFQNVCL
                     GLALWNGRDPILKERLFGLSGHEGNHGEDAKELWYHLDGTPTHSYMKMIYRYPQAEFP
                     YRRLVDESAARGYGDPEFELIDALPEDLRQGRFFDVTIEYAKHDEEDILCRVTAVNHG
                     PQPAPLHLLPQLWFRNTWSWDPRRADRPELVRVDGGPAGAATVRATHRHLGERLWYVE
                     QATVLLFTENETNTERLFGRPNGARRVKDGFHEAVVQGEAGRVGTDRGTKVAAHFPFT
                     LAPEERVVVRLRFCDRALERPFRGFDEIFRRRIAESDEFYAAIQRPGLDGERKALQRR
                     AWAGLLWSKQFYHYGVQPWLDGDAAGPRPAGQRLSGRNAGWTHLYNTDVLSMPDKWEY
                     PWYAAWDLAFHALPLAQLDPEWAKRQLVLLLREWYQHPNGQLPAYEWNFGDVNPPVHA
                     WAAWRVYKIARNLTGQADTDFLERVFHKLLLNFTWWVNRKDVDGRNVFQGGFLGMDNI
                     GVFDRSAELPPRFRLEQSDGTAWMAMYCLNMLAIALELARVRPVYEDVATKFFEHFVW
                     IAGAINGGRGGSGLFDEQDGFYYDRLRHEDGAVFPVKIRSFVGLIPLFAVETFEPETV
                     ELFPRFARRVRWFVDYRRSMIEQIASPIVPGEGGRFLLSLVGAARLRRILERMFDENQ
                     FLSPYGLRSLSREHRERPFRWELGGRKLEVRYDPAESSGGIFGGNSNWRGPVWFPVNY
                     LMIESLQRFHHYYGDAFEIEVPSGSGHHLTLADAAGELARRLIALFLRDANGRRPFNG
                     GDSLFDEDPRWRDLPLFHEYFDGDTGAGLGASHQTGWTALLARLIQQFG"
BASE COUNT         2428 a         5959 c         6029 g         2595 t
ORIGIN      
        1 ccacgagccc acgccgatgt cgcacgtccc gcgctcgagc acggtcagca cgaagctgtc
       61 gtagcgtcgg acggcccgca gcgcctcgcc gtcgcgctgg cacttccatc tcacgacctc
      121 gaccgacggc gtgcggcaga ccgtgaccac gcgcacgcag ctatcgaggg aagccggaca
      181 cgagctcaac gtgggaactg cttttggcgc acctgtcacc cgtgggcgta cgaacgaggc
      241 cagcctcggg tttcagccga tcgattagaa ttcgggacgg agagcgagcc cgtgcttcat
      301 gccggcgaca ccctgctgca ctatcgcgtc gtcgagcgca tcggcgaggg cgggatgggg
      361 gtggtgtgga gggcgaccga cacgcgcctc ggtcgcgacg tggcgatcaa gctgctcccg
      421 gagtccgtca ccgccgacga ccgacggctg gtgcgcttcg aacgcgaggc gcgcgcgctc
      481 gcggcactga agcaccccaa catcgtcacg atctacgccg tcgaacagac gggcgagaca
      541 tggttgttgg tgatggagta cgtggacggt acaccgctgt cggcgcagat cccggaccgc
      601 ggtctgccgc tcgggcgtct gctcgagttg gtgatcgcgg tatcggacgc cgtcgccgaa
      661 gcgcaccgcc acggcgtcgc gcaccgcgat ctcaaacccg acaacatcgt cgtcggtgcc
      721 gacgggcggc ccagggtgct cgatttcggc ctggcgaaac tgatcgatgt cggctccgcc
      781 ggcgccgatt cgttcgacgg caccgtgacc cgcgggcccg ggcagacggt cgccggggag
      841 tggctcggca cgccggccta catgtcgccc gagcaggcgg aggggcgacc ggccgatgcg
      901 cggtcggacg tattcgcgct cggggtggtg ctgtacgaga tggcgaccgg gcaacgcccg
      961 ttccgcgccg acaacccggc ccggctggtc acgtcgatcc tgaccgagcg tccccgctcg
     1021 ctcgccgaac tgcgtcccga cctgccggcc gcgttcgggc gcatcgtcga acactgtctc
     1081 gacaaggacc ccgatcggcg gttccagacc gcgggagagc tgcacgaccg gttggtcgcg
     1141 ctgcacgacg ggccgcggcc ggcgcaccgc gcaaggcgcg cggcatcgtc gctcgcaatc
     1201 gtcgccgccg cggcggccgt cgtcgccgcg ttgtggttcg gactcgcgcg cgaccggacg
     1261 gcgccggctc ccgccgcccc ggctccgccc actgcgcacg tcctgtcgca gctgaccttc
     1321 gatccggcga ccgaacaatg ggcagcgttt tcgcccgacg gcaacgcgct ggtctacgcg
     1381 aaggagaccg cgggcttctc caagctattc ctgcgcgatg tcgcgaccgg gcgaacgcgc
     1441 cagctcacgg cgggtgatcg cgacgacatc cagcccgctt ggtcacccga cggagcgatc
     1501 gtcgtcttcg tgcgtgcctc gaacgagcgc ggcaagctcg aaccgagcga ggcgctggcc
     1561 ggcgtttacg acgacggcgc gctgtgggcc gtcgacgtga gcgacggccg cgagacgctc
     1621 gtcgtcgacg acgccttcaa cccggcgttc tcgccggacg ggagccggat cgcggtcgat
     1681 gcgtcgtggg ccggcccgcg acgaatctgg acgaccgacg cccgcggtcg caatccgcag
     1741 caggtcacga ccggcgagtc cgaggcggtg cagcatacgc agcccgcctg gtcgcccgac
     1801 ggtgcgcgca tcgtcttcca gcagggcgag aagaccaagc tcgacctgcg ggcggtcgat
     1861 gcaacgggcg gcgagagcat cgccgtcacg gacgatctgt attccgacct cgatccggta
     1921 tggtccgccg acggcggtca catctacttc tcctcctatc gcggcggcgg gctcaacatc
     1981 tggcgcgtcc cggtcgccgc cgatggccgg ccggtgggtg cgccgcagca actcacgacc
     2041 ggcgccggtc aggacatcca ggcctcgctc gcgccggacg gcgatcgtct ggttttcacc
     2101 gtgttgtcgc agaacgccga cctgtggcgg ctgccggtcg atcccggcac cggtgccgtc
     2161 accggagaag cggagcctat cgtggtcagc acgcgcgagg acagccgcgg cgcctggtcg
     2221 cccgacggtc gccggctggc gttcaactcc gatcgcgacg gcacgatgaa cgtctggatc
     2281 cactcgctcg ccgacggggc caccgagcgg ctgaccagcg gccccggcgg agactaccag
     2341 ccccgttggt cgcacgacgg tcgctcgatc gctttcttct ccgcgagatc ggccaacgcc
     2401 gaaatctggc tggtcgacgt cgagacgcgg gtcatacgcc agctgaccga ccaccccgcg
     2461 ctcgacacca acccgttctt ctcgcccgac gatcggtcga tcgcattcca atcggaccgc
     2521 gacggacgca aggaggtgtg gctcgtcgcg gtcgacggtg gcgaaccgcg acggttgtcg
     2581 accgtaggcg cagccgatca cttcatgcgc tggtccgacg acgggcggct ggtctacttt
     2641 cgctcctccg cccgaccgga gaacggcatc tgtcgcgtcc cggtcagcgg cggggaggcc
     2701 gattgtctcg gcgttccgct gggctggcac atgtcgttct cgccagaccg gacgcgggtg
     2761 atggacgtgg tcggccacaa ggggctgtgg gtctacccga tcgacggcgg cagcggcgtc
     2821 gaggtccacc ggtcggcgga gcccgacgtc cgcgtcgact accccgagtg gtcgcccgac
     2881 ggccgttggg tcgtctacga ccgcgtcgtg ccgcgcggag gcgacgtttg gctactcgac
     2941 ggtctcaccg ccggcggccc gcgcgcgcgc tagcgctcct gcgccggccc gaccgaccga
     3001 gccgtcgttc gcaatcaccg cttcgactcg atcgtctacc tggtgaggcc cgaccccacg
     3061 acgggcgcac gaggacccga tgccacgatc ccatccgagc gtccgatcgg gaccagacga
     3121 ggcggaatcg gccgaccggc gggatctcga cacggatcgg acaaccgatg cgacgacctc
     3181 gcttctgcgc cagggctatc gcttcgccct ctcactgacg cacgaccggg ctcgtgcgga
     3241 ggacctgctg cacgatgcct ggatcgccgt gcgcaaggcg aacggtcctc gaaccgcacg
     3301 ttacctgatc acggcgattc ggtcgtgctt cgtcgatcgg tgtcgccggg cactgatcgc
     3361 gccgatggag tcgctcgaca ccggaccgca gcgggcagcg aacgccgagg atctgtcgtg
     3421 ggacgatccg tatgacgccg tggcgatgaa gtccgcgctc gagcgggcgc tgggtctgct
     3481 gcggcccgaa gagcgtgcgg tgctgttgct gtccgctgtc gagggataca cggcgcgcga
     3541 gatcggcgag ctgctcgacg cgccgcgtgg aaccgtcctc agcctgatgc atcgcacgcg
     3601 cgccaagatg cggcgctggc tggcggaccc gaccgattcg gaggaacgac gatgacgaac
     3661 gacgatcgaa cgctcggaac cctgcgggat gcggtccgcg attactacga cggccaggcc
     3721 gtcgacgacg ccgtgctgcg gcggctgatg gccggccggg agcgggacga gcgcccgtgg
     3781 cgctgggcga tcgccggagc gctggccgcg atgctcgttc tcgcggcgac cggtgtcggt
     3841 ctgaaaggaa gcgcctggct cccggcgcgc ggcgacgccg aggcaacacc ggcgcttcca
     3901 cgactcgtgg cggtgcagat ccgcgcggac tggtgtgcgc gtacgccgga ggtgggcccc
     3961 gtgttcgcca ggctggtgag ccagtacggc ggcgagccga tcctgttcgt caccgtggac
     4021 atcagcgacg acgctcgacg ggagcaggcc gcgctcctga cggcgaacct cggcatcccc
     4081 gaggccctcg aggccccgtt cgagtcgggg atgatcaagc tgctcgatcg cgaccggcgg
     4141 cggttgctcg cggcggtgac cgaccctgct cagctcgagc agttcgagac ccggatcgcc
     4201 gaagcgctct ccggcgcgaa gggcgacgcg aggggcggcg cctgacgatc ccgaccgctc
     4261 gaccgagccc tggggtcccc gcctgatcgc gtgcgggtcg cggtcgccgt tccgctcctg
     4321 ggacgcgcgc gtcccggaag cggacacttt cggttcgtcg ggccgcgatt ccggcccgtc
     4381 gcctctggca tcgtctttgc gcacccaggt cgcggatgga cgttgcgcgg cccaggaaga
     4441 agcgttatct cgcccggggt ctgaccgtcg gtgccggcgt cggcatcctc ggcgccgtga
     4501 ccgtcggcct gtacggcatc tcgtctcccc cgcccaccgt cgtgcgcagc gcggtctgga
     4561 tcgacaccgt gcgacggggg ccgatggtca tcgaggtccg cggggcggga acgctcgcac
     4621 ccgagcaggt gcgctggatc gtcgccgaca ccgcgggccg ggtcgaagcc cggctgctcg
     4681 acgcgggcgc cgcggtcgcg gccgacaccg tgctggtcga gttgcgcaac cccgagctcg
     4741 agctcgccgc ccagcaggcc ttgctcgagt tgcgagccgc cgaggccgag acgcagagcc
     4801 tgcgagtcgc gctcgagcac gagttgctcg accaggaagc cctggccgcg accgtcgagg
     4861 ccgattggcg ccaggcgcag ctcgaagtgg agtcgaacga agagctggcc aaggacgggc
     4921 tcgtccccca gctcacgctg aagatcgcgc gcgtgcgtgc cgacgagctg gcgctgcggc
     4981 gtggcctcga acaccgacgc ctggccagcg ccgaacgcgc gaccgccgcc cgcctggcgg
     5041 cgcagcaagc gcgcgtggac cagctcgccg cggtcgccgc gctgcgccgt cgcgaggtcg
     5101 ccggcctcga ggtccgtgcc ggcacggcgg gggtgctcca ggacatcccg gtcgagccgg
     5161 gccaatcggt cgccgccggc gcggtgatcg cgcgcgtcgc ggagcccgag cgcttggagg
     5221 cgcgcctgct gatcccgcag agccaggctt ccgaggtcca gctcggccag ccggcgacga
     5281 tcgacacgcg caacggcagc gtctcgggca gcgtcgtgcg catcgacccc gccgtacgca
     5341 acggcaccgt gaccgtcgac gtcacgctcg agggcgagct gccgcgaggt gcgcgccccg
     5401 acctgacggt caacggcacg atcacgatcg agcacctgac cgacgtgctg cacgtcggcc
     5461 gccccgcctt cgctcccgcg ggcgagccgg tgggtctgtt ccgcctcgtg ccggaaacga
     5521 acgatgccga gcgcgtcgag gtcgtcttcg gccggtcctc ggtccgactg gcagaggtcg
     5581 tctcgggcct ggccgaggga gaccaggtga tcctgtccga cgttcccgga ttgggtgacg
     5641 cgaagaccgt gcgcctgcag taaggggagg aagagcaaga tgacgtcgtt cctgttcgat
     5701 ctgcgcttcg ccgcgcggtc gctggcccgc aatccggggt tcgcggggat cgcgatcctg
     5761 acgctcggtc tgggcatcgg ggccaacacg gcgatcttca gcgtcgtcga ggcggtgctg
     5821 ctgcgcccgc tgtcctacgc cgatcccgag cggatcgtgc gcgtctacac ggagttcccg
     5881 acgatgagct tgccgcggtt ctgggtttcg ccgcccgagt accgcgagct gcagcgcgac
     5941 gcgcgctcgt tcgaggcgct cggggcgtgg gccgaggccg ggacgaacct cggcggcgac
     6001 gacggcccgc tgcgcgtgtt ctcggtctac gcgacacacg agctgttcga cgtgctcggc
     6061 gtgcccccgg cgctgggccg cgtcttcggt cccgaggaag acgtccgtga cggcccgctg
     6121 gccgtgatcc tgtccgacgg cttgtggcga cgggcctacg gcgcgcgcag cgacatcgtc
     6181 ggcgcagagg tgctggccga cggcatcccg gcgacggtgg ccggcgtcat gccacccggc
     6241 ttcgagttcc caccgggctc ggccacgccg gccgaggcgt ggctgccgct gcagctcccg
     6301 cccgagccgg aaaaccgctc gaaccactcg ctacacgtaa tcggtcgcct ggcgccgaac
     6361 gtgacgatcg agcaagcccg cgatgaggcc gaccgcctga tcgatgcttg ggtcggcgga
     6421 gccggcgacg cgccgcagct gtacaacccg agccacggcc accgcctgaa cgagaatcac
     6481 ccgctgctgc tgcaccgcta ccacgacgag gtcgtgcgcg cggtgcgccc ggccgtgttg
     6541 atgctgatgg gcgcggtcgg cttcgtgctg ctgatcgcct gcgtcaacgt cggtaacctg
     6601 ctgctcgccc gggccgaggt gcgccagcgc gagatcgcca tgcgggccgc gctcggggca
     6661 ggcacggcgc gactgctggc ccagttcctg gccgagggcg tgctgctcgc cgcggccggc
     6721 ggcggggtgg gaatcctgct ggccaactgg ggcctggccg gcctcctcgc cgccgacccg
     6781 agcggcctcc cgcgcgcggc ggaggtcacg atcgacgggc gggtgctctt gttcacgctc
     6841 ggcgtgtcgc tgctcgccgc ggtgctgttc gccctcgccc cgctgaccca cgtgcgcgga
     6901 atgcggctgg cctcgcgcct gcacgagggc ggccaccgcg tggccggcac gctgtcgggc
     6961 cagcgtctgc ggcgggcact ggtcgtggcc caggtcgcgc tgaccgtggt gctcgtcaca
     7021 ggcaccggcc tgatggtgcg tgcgttctgg aagctccagg acgtcgaccc cggtttccgg
     7081 ccgtcgggcg tgctgaccgc gcaggtcgag ctggtcggac cggcgttcga gaccgccgag
     7141 gcgcgacacg ctttctgggt ctcgctccaa gagcgcctgg ccggcgtgcc cggagtcgcc
     7201 cgggtgacca tcgccagcgg cctctacccc gaacggccgc tcaacgccaa cgacaccgac
     7261 ttcgagggct atgacccgac ctccgacgag gacccccacg agaacgtcga cttctaccag
     7321 aacgtcggtc gcgacttctt cgataccgca ggcatcgcga tcgtcgaggg gcgcgacttc
     7381 gaggaagccg accgcgcgcc gggcgccgcg ccggtcgtca tcgcgaacca ggctctggcc
     7441 cgccggttct tccccggcgc gaccgcgctc ggccagcgcg tgcgtgtcgg tggcggcgag
     7501 aacccgtgga tgacgatcgt cggcatcgcc gccgacgtcc gcaactacgg catggacacg
     7561 ccggctcgcg ccgagctgta cttcgacctg ggccaggcac tccagtacgg attccaaccc
     7621 agcctgctca acctgctcgt gcggaccgac ggagacccgc gcccgctcgc gccgtcgatc
     7681 cgcgccgccg tgcgcgagat cgacccgcag gtcccgctgt tccgcgtgcg cacgatggac
     7741 gaggtcgtcg cggcggcgat gagccggccg cgcttcctgg cgctgttgct gacggtgttc
     7801 tcggcgctgg cgatcgcgct ggcggcggtc ggcatctacg gcgtggtttc ctaccaggtg
     7861 gcccagcgcc ggcaggaaat cggcgtgcgc gtcgcgctcg gcgccgatcg cccggtgatc
     7921 ctgcgcatgg tcatggtcgc gggcctgcgt ctggtcgtgc tggggatcgt cctcggcgtc
     7981 gggggcgcga tcgcgctgac gcgcgttatg caggggctgc tgtacggggt cagcccgctc
     8041 gatgcgccgt cgtttctggg agcgaccgtg ctccttttgg ccgtcgccgc ggccgcgtgt
     8101 tggcttccgg cgctggccgc gacccgggtc gatccgatgg tcgcgctgcg caccgagtaa
     8161 ccgtcaagga gggatcgtga gcacaccaaa ccaagagaac cgcccgctga tccgcttgga
     8221 ggcgctgacc aaggtcttca tcgccgacga gatcgagacc cacgcgctgt cgaacgtcca
     8281 gctgacggtg actcgcgggg agtacgtttc gatcgccggc ccctcgggct gcgggaagac
     8341 cacgctgctg tcgatcctcg gcctgctcga cgcgcccagc cacggacgct actggctcga
     8401 cgggtccgac gtggccgccc tcggttccgc cgcgcgggcc aaggtgagaa accgccagat
     8461 cggtttcatt ttccagagct tcaacctgat cggcgagctg acggtggccg agaacgtcga
     8521 actgccgctg acctaccgcg ggctctccgc cagcgagcgc ggcaagcggg tcgctcaggc
     8581 gctcgagcgc gtggcgatgg gtcatcgtgg gcgccactac ccgtcgcaac tgtcgggcgg
     8641 ccagcagcag cgggttgccg tcgcgcgcgc actggtcggc gaaccgtcga tcctgctcgc
     8701 cgacgagccg acggggaacc tcgactcgca caacggcgag gcggtgatga ctctgctcga
     8761 cgacttgcac cgggcgggtt cgacgatctg catggtgacg cacgacccgc gcttcgcgcg
     8821 ccacgcgcag cgcacggtgc acctgttcga cggtcagatc gtcgaggagc gcgcggcgta
     8881 gaataggaag catggctgcc ccctccgagg tcgcggcgcg ggccgcgcgc gtgctggtcg
     8941 ccgatgaccg ccccgacgtg cgcgaggcgc tccgtctgct gctgcggccg gagggcttcg
     9001 aggtcgaggt ggtcggctcc ccggccgggg tgctcgccgc cctcgaagcg cgcgatttcg
     9061 acgtcgtgct gatcgacctg aactacaccc gcgacaccac gtcgggcgaa gagggcctgg
     9121 acctgctgcg gcgcatccgc gcgctggacg cgcatctgcc ggtcgtggtg atgaccgcct
     9181 ggggcagcgt cgagttggcc gtcgaggcga tgcgacgcgg cgcgcgcgat ttcgtcgaga
     9241 agccgtggga caacgcgcgt cttctcgcca cgctgcgcac gcagaccgac ctcggccggg
     9301 ccctgcgctc ggaagagcgc ttcgccgtcg cgagccgtct cgccaccggc gacggcgatg
     9361 ccccgctggt cgccgagtcg gccgcgatga ccccggtact ggagctggtc gagcgcatcg
     9421 gcccgtccga cgcgaacgtg ctgatcaccg gcgagaacgg aaccggcaag ggccgcatcg
     9481 cgcgcgcgct gcacgccgtg tcggcccgct cgctgcggcc gttcgtcgtg gtcaacgtcg
     9541 tcggcttggc cgagggcgtg ttcgagagcg aactgttcgg ccacgtccgc ggcgccttca
     9601 ccgacgcccg ggccgaccgc atcggtcgcc tcgaggcggc cgacggcgga acactgttcc
     9661 tcgatgagat cggccacctg cctgcggcac tacaggccaa gctgctgcgc gtgctcgagc
     9721 ggggcgaatt cgagcgggtg ggctcgtcgg cgacccgccg gctcgacctg cgcatcgtgt
     9781 cggcgaccaa cgcggagctc gacgtcgaag tgcgcgaggg gcggttccgc caggatctgc
     9841 tgttccggct caacacgatc gagatccgac tgcccccgct gcgcgcgcga cgcgaggacg
     9901 tggtgccgct cgcgaactcg ttcctcgcgc gcgcggcgcg ccgctacggc cgcgacgtgc
     9961 gcggcttcga gccggaggcc tacgaggcga tggtcgccca tccgtggccc ggaaacgtgc
    10021 gcgagctcga gcacgccgtc gagcgcgcgg tgctgctcgc gagcggatcg gcggtccgcg
    10081 agccggatct gcggctgcga ccgccggcca cggcgtcggg cacgacgccg gtcgccgggg
    10141 ggctcgcgga gctgagcctg gaggaagtcg agcgcctgtt gatccgcaag gcgctcgacc
    10201 gccacggcgg taacgtcaag caggccgccg atgcgctcgg cctgagccgg agcgcgctgt
    10261 accgtcgatt gcagcgccat gggctctgaa ccgcggcacg cccgacgcgg ctgggaacgg
    10321 cgcttgcgag acaccgccct gttgggcggg tccggggcgg tgctggccac gatcgtgctg
    10381 ttgtggaccg ccgacctcgc gccgcgcacc cgctggaccg tcgtcctggc cgccgtcgtg
    10441 ccgtggctcg tcgcggcgtg gaccgtcgag gaccgggcgg caagaccgct gcgcgcgctg
    10501 gcgaacctgc tcgaggcgct gcgtgcgggc gacacctcgt ttcgcctgcg ctcggagcga
    10561 ccgggtgacg cgctcgacga agtcgtgcgg gaggcgaacg cgctccgcga catgctgcgc
    10621 ggcgaacggc tcgccacgcg ggaggcgacg gcgctgctgc gcgcggtcac ggaggagatc
    10681 gacgtggccc tgttcgtcct cgatgcggag cggcgggtgc gttttgccaa ccggcgcggg
    10741 gcgcggttgc tgggcaagtc gcccgaggcg ctggccggga tgggcgccgc cgaggcgggc
    10801 ctccgcgcgg ccgtcaccgc aggaggtgcg gcggtgcgac cgatcgactt ccccggcgac
    10861 agcggccgct ggcaggtgcg tcgcgtgccg ctgcgcgagg gcggcctgcc ccgcgagctg
    10921 gtaacgctga gcgacgtcgg cccgaccctg cgcgaggagg agcggcaggc ctggcagcgc
    10981 ctgatgcgcg tcctcggcca cgaggtgcgc aactcgctgg ccccgatccg ctcgatcgcc
    11041 gagggcctgc gcggtcggct cgcccggcgg gaggacgccg cggaaacccg ccgcgacctc
    11101 ctcgacggcc tggcgatcat cacggatcgc tcggcctcgc tcggtcggtt cctcgagggc
    11161 tacgcccgac tcgcccggct gccgcggccg gttctcgccc cggtcgaggt cgggctgttc
    11221 gtccgggatg ccgcacgtct cgagacccgg attccggtgc aggtggacga aggcgcgcct
    11281 gtcaccgtgc gcgccgaccg cgcacaactc gagcaggtac tgatcaacct gctccgcaac
    11341 gccgcggatg cgtcgatcga aacggggggt ggtgtacgtc tcggctgggc gacggtacgc
    11401 ggtgccctcg agatccacgt cgaggacgac gggccgggcc tcggcgcggc ggagaacctg
    11461 ttcgtcccgt tctacacgac gaagcccgac ggcacgggta tcggcctcgt cctgtcgcgc
    11521 cagatcgtcg aggcacacgg tgggacgctg acgctcgaga accgccgagc cgtggcgggg
    11581 tgcctggcga ccctgcggtt gccgctctga cgcgacgtcc gcgcggcgcc ccgcgccccg
    11641 tgcattccat cgcgcccggc ctatcttgtc ctgcagtcgt ccggctccgg ctgcgagggc
    11701 aagatgatcc tgataccgat cggccacgag gaagaggcgc gccggctgcc gtgggtgacc
    11761 ttcgtgatcc tgggtctgtg cgggctggcc ttcctgctca ccggcttcgg cggcacgcgg
    11821 agcgagtggg acacgctcga atcccacgag cgggcgatcg agtactggct cgagcacccg
    11881 tacctgcggc tcgatcccga gatcgagcgt gcgatgcacg gaatggcgac gcaggagatg
    11941 cgggacacgt tccgggaggc gatccgcacg aacgtcgacg tgcccgcagc ggagaccgtg
    12001 agcgtcgagc agctcgagct ggaccggctg tgcgagcacg cgaagaacgc atcccccgat
    12061 catccgttct accgctttgg tctcgttccc tcatgcatcg gccccgtgac cctgatcacc
    12121 cacatgttcc tccacgccgg ctggctgcac ctgctcggca acctcttcat tctctacctg
    12181 tgcgctccgt tcgtggagga cgtctggggg cggccgctgt tcgccggctt ctatgccgtg
    12241 tccgggatcg tggccgcgct gaccttcgcc ctgcctcatt cctcgttgac cgagccgctg
    12301 gtcggcgcct ccggagcgat cgcaggcgtg atgggcgcgt tcgccatacg gtatgcccgg
    12361 acgcgcgtgc agttcttcta tgcgatcgga ttgatgaccc gtgggacgtt ttgggccccg
    12421 gcgtggttca tgctgggact gtggttcgcg cagcaggtct tcctggccat gctgacctcg
    12481 aacacggatg gcccgggtga caaggtggcc tacctggcgc acgccggagg cttcgtcttc
    12541 ggcgccgcgg tggccttcgg catgaaccgg tatcgggtgg aggagcgctg gttggccggc
    12601 gcgatcgagg cccgggccaa cacggtcgtg atgcggaacg agacggtgga tgcggcgctc
    12661 ggggccgccg cggccggcga cccgacccgc gcctgggaac tcctgaccgg cgagctgcgc
    12721 cggaacccga ccaacgtgga tgccgccatg gcgctgtgga gcgtgagtca ggacgccggc
    12781 cggcagagcg aagccgcccc ggccttcgca cgcatgatcg agcaccaggt gcgaacgggg
    12841 aacctcgatg gggcgctggc ccactgggag gagctccgcg ccgcggtccc gacgacgatg
    12901 ctcgatgtgc gcaccctggt gcggatcgcg ggcgcctacg tcgagggcgg ccatcgcaag
    12961 gaagccgccg acgccctgcg ccgcgcgctg ctcggcgccg gaggtgcccc gaacccgacg
    13021 atcctgctgc gcatcgccga gaccgcgacc ggcatcgatc ccgcgctggc acgcgccgcg
    13081 gccacggtcg ccctgggccg ctcggatctc gacgaggcct cacgcggtcg ggcgaggagt
    13141 ctggccgagc cgtccgccac ccgaacctga ggacgaggtc gagcggttcc ccgctactcc
    13201 tcgaacgcgc tggcgacggc ctcgccgtag gttccgtcga aggcgcggcg gaagccttcc
    13261 acgagtcgga ggttgatggc gttcatcggg ttgtcccgct gccggtccag cacgcgatag
    13321 ctggcttcga gcgtctcgcg gtctttctcg agaaacgcga tggtcgcccg cacgtacgcg
    13381 ttccacttgc cccttccggc cggatcctcg tgcggattga aaccgcgata gaaatgcgcc
    13441 agtgcccgcg aggtatcgtt ggcgcctgca aactgctggc cggcatggaa ttccgagacg
    13501 tagcgttgct gcaccgtgag cccggggtgt gcgtcgaggt agctctcgat cagttcgccg
    13561 gtttcccgcg ggcagtagcg ctgctcgctg acgaacgcgc gaaagccctt cggcccctgg
    13621 tcgaaggctt cccagtcgag ggccagctgc cgcgcggtct cttcggccgt gatcaggcag
    13681 ggctggctgg ccagcagcag cgtgaatacg agtccgaaca ccgggtcagc ggaccccgat
    13741 cgtgaccttc ttgatctcga gcagctcgtt ggggcggccg ctgctcgagc cggcggcctc
    13801 gagcgccttg agcgtctcca tcccgtcggt gacctcgccg tagatcgtat gcttgccgtc
    13861 gagccacggc gtgggcacga aggtcaagaa gaactggctg ccgtcggtgc cgggcccggc
    13921 gttggccatc gacagcagac cgccgcggtc gtgcttgacg cccggcttga actctccgtc
    13981 gaactcgtag cccggaccgc ccgtgccgga accgagcggg catccgccct gcgccatgaa
    14041 cttggggatc acccggtgga acttcaatcc gtcgtagaag ccgagacgcg tcaagtagat
    14101 gaagctcgtg acgtgcatcg gcgcgacggt cggcatcagc tcgatctcga tcggaccctt
    14161 cgtcgtctcc atcgacgcga agtaggtctt gccttccgga aacgtcacca tcttcggcag
    14221 cggtaggctg gtacgccagt tggccgactg cttgtcgaca ccggccttgc tgatttgctc
    14281 gtcgatctgc tggaacacga tgtcctcctg cgctttgtcc tcggcggaaa cgcccgcccc
    14341 gaccggcagg accgacaaga cgatgacgat caacgctctc ttgaacatga agatcctcca
    14401 gacctcggtg atgggaaacc cgaggagtct accaccggtc ggccgcgact gccggggccc
    14461 ggctatcagc cgaactgctg gatcagccgg gccagaagcg cagtccaccc ggtctggtgc
    14521 gaggcgccga gtcccgcgcc ggtgtcgccg tcgaagtact cgtggaacag cggcaggtcg
    14581 cgccaccgcg gatcctcgtc gaacagcgag tcgccgccgt tgaacgggcg ccggccgttc
    14641 gcgtcgcgca ggaacagcgc gatcagtcgg cgcgccagct cgccggccgc gtcggccagc
    14701 gtcaggtggt ggcccgagcc cgacggcacc tcgatctcga atgcgtcgcc gtagtagtgg
    14761 tgaaagcgct ggagcgactc gatcatcagg tagttgaccg ggaaccaaac cggcccgcgc
    14821 cagttggagt tgccgccgaa gatgccgccg ctcgactccg ccggatcgta acgcacctcg
    14881 agcttgcggc cacccagctc ccagcggaac ggtcgctcac ggtgctcgcg cgacagcgag
    14941 cgcagaccgt agggcgagag gaactgattc tcgtcgaaca tccgttcgag gatgcggcgc
    15001 agtcgcgcgg cgccgacgag cgacagcagg aaccggccgc cttcgcccgg cacgatcggc
    15061 gaggcgatct gctcgatcat cgatcgacga tagtcgacga accagcgcac ccgccgggcg
    15121 aagcgcggga acagctcgac cgtctccggt tcgaacgtct cgacggcgaa caacgggatc
    15181 agaccgacga acgaacggat cttgaccggg aagacggcgc cgtcctcgtg ccgcagccgg
    15241 tcgtagtaga aaccgtcctg ctcgtcgaac aaaccgcttc ctccccggcc gccgttgatc
    15301 gcgccggcga tccagacgaa gtgctcgaag aacttggtgg cgacgtcctc gtacaccgga
    15361 cgcacgcgcg caagctcgag cgcgatcgcc agcatgttca ggcagtacat cgccatccac
    15421 gccgtgccgt cggactgctc gagccggaag cgtggcggca gctcggcgga gcgatcgaag
    15481 acgccgatgt tgtccatacc gaggaagcca ccctggaaca cgttgcgtcc gtcgacgtcc
    15541 ttgcggttga cccaccaggt gaagttcagc agcagcttgt ggaagacgcg ctcgaggaag
    15601 tcggtatcgg cctgtccggt gaggttgcgc gcgatcttgt agacgcgcca ggcggcccac
    15661 gcgtggaccg gcggattgac gtcgccgaag ttccactcgt aggccggcag ctggccgttg
    15721 gggtgctggt accattcgcg caacagcagc accaactggc gtttggccca ttcggggtcg
    15781 agctgggcca acggcagggc gtggaacgcc aggtcccacg cggcgtacca cggatactcc
    15841 cacttgtccg gcatcgacag cacgtcggtg ttgtacagat gcgtccagcc ggcgttgcgg
    15901 ccggaaagac gctggccggc cgggcgcggg cccgccgcgt cgccgtcgag ccatggctgc
    15961 acaccgtagt ggtagaactg cttgctccac aacagcccgg cccacgcccg gcgctgcagc
    16021 gccttccgct cgccatcgag cccggggcgt tgaatcgccg cgtagaattc gtcgctctcg
    16081 gcgatccgcc gccggaagat ctcgtcgaag ccgcggaacg gccgctcgag cgcgcggtcg
    16141 caaaagcgca ggcgcacgac gacccgttcc tccggggcga gggtgaaggg gaagtgcgcc
    16201 gcgaccttgg tgccgcgatc ggtgccgacg cgtcccgctt cgccctggac caccgcttcg
    16261 tggaacccgt ccttgacccg cctcgccccg ttgggccgcc cgaacagacg ctccgtgttc
    16321 gtctcgttct ccgtgaacag cagcacggtc gcctgctcga cgtaccacag ccgctcgccg
    16381 aggtggcggt gcgtcgcgcg caccgtcgcg gcgccggccg gtccgccgtc gacccgcacg
    16441 agctccgggc ggtccgcgcg gcgcggatcc cacgaccacg tgttgcggaa ccacagttgg
    16501 ggcaacaggt ggagcggcgc cggttgcggc ccgtgattga ccgcggtcac gcggcacagg
    16561 atgtcctctt cgtcgtgctt ggcgtactcg atcgtcacgt cgaagaaccg accttggcgg
    16621 agatcctccg gcaacgcgtc gatcagctcg aactcgggat cgccatagcc gcgcgccgcc
    16681 gattcgtcga ccaaccgccg gtaaggaaac tccgcctggg gatagcggta gatcatcttc
    16741 atgtacgagt gcgtcggtgt gccgtcgagg tggtaccaca gctccttcgc gtcctcgccg
    16801 tggttgcctt cgtgcccgct caggccgaac aggcgctcct tgaggatcgg gtcgcgcccg
    16861 ttccacaacg cgagaccgag acagacgttc tggaatcggt tgcagaaccc gccgagcgcg
    16921 tcttcgttcc agcgataggc gcggctgcgc gcgtgatcgt gcgggaagtg gccccaggcg
    16981 tcgcccgtcg cgctgtagtc ctcgcggacc g
//
LOCUS       DASPGN010000598         4138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1284011, whole genome shotgun sequence.
ACCESSION   DASPGN010000598 DASPGN010000000
VERSION     DASPGN010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284011"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VD788_17370"
     CDS             complement(<1..701)
                     /locus_tag="VD788_17370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV78084.1"
                     /translation="MEPPGTNRELIFARHQINQGQILGAIETLKRVLARDPNDAVAHG
                     LLAVCLLDAKRPHGAEHEARECLRLDPESPIGHYAMGSVALARRRFDEAQRSFETLLE
                     LEPELAENQRAMAALARARGKTPEAEERLRQALELDPEDPTTLTELGTLRLAAGDVDG
                     ADARAREALALAPEHLDALVLKGAVELRRGQVGSAREHAVWALRLNADDDGALRLMAS
                     IKSRTNRLLGLWWRY"
     gene            complement(701..1174)
                     /locus_tag="VD788_17375"
     CDS             complement(701..1174)
                     /locus_tag="VD788_17375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78085.1"
                     /translation="MSATYRIPDEPRPSTLSRFAVQPFWPLLAVMFGGSWLSWPWFVF
                     NGFAIGSPTRKKELALAAAGFAGNVVLALGVGIVVVRDLLPGTVGPYLALLGVVWRLG
                     VSYLLFNLQSRTFGLYVYYGGVVQNGTIFLVAGYLLGRTLLPRVVDAVPLLRLVI"
     gene            complement(1179..2525)
                     /locus_tag="VD788_17380"
     CDS             complement(1179..2525)
                     /locus_tag="VD788_17380"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXV78086.1"
                     /translation="MVNDETLRSLEAAYAASPDNVKLCLVLALAHAERGERAEAAALV
                     AQASWDEAPLEDRLSAAAILLAGDRYEDALRLAGEDEPRALLVQARARFGLGQNERAR
                     SAYQRAVAANPTLEDPALAERLETRTRVIPSAEGRPRLRVISNDDTVAAEADRLLVPE
                     PESIDFSGVGGLDDIKKQIHRKIILPFQKPSLFERFRKRVGGGILLYGPPGCGKTLLA
                     RATAGECSATFFNVAISDVLDMYIGESEQKLHALFEKARNSVPAVLFFDELEALGGKR
                     QYNREAVSSKLVSQFLSEMDGFTRNNKGVLILGATNVPWAVDSAFRRPGRFDRVLFVP
                     PPDRPARRRILEILLDRRPIADDIDLDDLAERSSGFSGADLENVVETAADLAIERSIS
                     SGAESPIGSRQLREALAELRPTTVEWLTTARNYARYANESGHYDEVLAFLARHGKS"
     gene            2955..>4138
                     /locus_tag="VD788_17385"
     CDS             2955..>4138
                     /locus_tag="VD788_17385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV78087.1"
                     /translation="MDWTPERFAELDRLFQQALELEPAAREAFVARQQQRSPETGRRL
                     REMLRADGRTGASIDEIVAAVADAFPPERDRLVGERFGPYRAERLLGRGGMGAVYLGE
                     RIEGDFAQTAAIKTVRFGLESPQLLDRFRREREILAQLDHPGIARLLDGGPGPHGLPF
                     VAMEYVDGLPITEYVRREKCGLVRRLELFAELCDAVAFVHRNSIVHRDIKAGNVLVTR
                     EGRVKLLDFGIAKLDDAFDLAGLTRTEQRPMTPEYASPEQVLNQPITTASDIYSLGVL
                     LYELLTDVRPVRLTSREPYAMAKAIVDQIPALPSQAASPLPPWQRRLRGDLDRIVMTA
                     LRKEPERRYASVTALADDVRRYLAGRPVSATADVWHYRLRKWLGRHPVAGAVAVIFLV
                     TVG"
BASE COUNT          639 a         1462 c         1420 g          617 t
ORIGIN      
        1 ctgtagcgcc accacagccc gagtaaccgg ttcgtccgcg acttgatcga cgccatcagc
       61 cgcaacgcgc cgtcatcgtc tgcgttgagc cgcagcgccc agacggcgtg ctcgcgcgcc
      121 gagccgacct gcccgcgacg aagctcgacg gctcccttca gcacgagcgc gtcgagatgt
      181 tcgggcgcga gcgcgagcgc ctcccgggcc cgcgcgtccg cgccgtcgac gtcgcccgcc
      241 gccagccgca gcgtgccgag ctccgtcagc gtcgtcggat cctcggggtc gagctcgagc
      301 gcctggcgca gccgctcctc ggcctccggc gtcttcccgc gcgcccgcgc cagggcggcc
      361 atcgcccgct ggttctccgc cagctcgggc tcgagctcga gcagcgtctc gaacgatcgc
      421 tgcgcctcgt cgaatcggcg gcgggcgagc gcgaccgatc ccatcgcgta gtgaccgatg
      481 ggcgattccg ggtcgagccg caggcactct ctcgcctcgt gctccgcgcc gtgtggccgc
      541 ttggcgtcga gcaggcaaac cgccagcagg ccgtgcgcga cggcatcgtt cgggtcgcgc
      601 gccagcacgc gtttgagcgt ctcgatcgct ccgaggatct gaccctggtt gatctggtgc
      661 cgggcgaaga tcagctcgcg gttcgttccc ggtggttcca tcaaatcacc agtctcagca
      721 acggaaccgc atcgaccacc cgggggagca aggtcctgcc gagcaagtag ccggcgacga
      781 ggaagatcgt cccgttctgg accacgccgc cgtaatagac gtagagaccg aaggtccgcg
      841 actggaggtt gaacaacagg tagctcacgc ccaagcgcca gaccaccccc aacaaggcca
      901 ggtacggacc gacggtgccg ggcagcagat cgcggacgac gacgatcccg acgccgagcg
      961 ccagcacgac gttgccggcg aagcctgccg ccgcgagcgc cagttccttc ttccgcgtcg
     1021 ggctgccgat cgcgaagccg ttgaagacga accagggcca ggacagccac gagccgccga
     1081 acatcaccgc caacagcggc cagaacggtt ggacggcgaa gcgagacagg gtgctcggtc
     1141 gcggctcgtc cggaatgcgg taggtcgcgc tcatcgggtc aggacttgcc gtgccgggcc
     1201 aggaacgcga gcacctcgtc gtagtgcccc gactcgttgg cgtagcgcgc gtagttgcgg
     1261 gcggtcgtca gccactcgac cgtcgtcggt cgcagctcgg cgagcgcctc gcgcagctgc
     1321 cgcgatccga tcggcgactc ggcgcccgag gagatcgatc gctcgatcgc caggtcggcg
     1381 gcggtctcga cgacgttctc gagatcggcg ccggaaaaac ccgagctgcg ttcggccagg
     1441 tcgtccaggt cgatgtcgtc ggcgatcggt cgccggtcga gcaggatctc gaggatccgc
     1501 cgacgagccg gtcggtccgg cggcgggacg aacagcactc gatcgaatcg tcccgggcgg
     1561 cgaaaggccg agtcgacggc ccacgggacg ttggtcgcac cgaggatcag cacgcccttg
     1621 ttgttccggg tgaagccgtc catctcggac aggaactggc tgaccagctt ggaggagacc
     1681 gcctcgcggt tgtactggcg cttgccgccg agcgcctcga gctcgtcgaa gaacagcacc
     1741 gcggggacgc tgttccgcgc cttctcgaac agtgcgtgca gcttctgctc ggactcaccg
     1801 atgtacatgt cgagcacgtc cgagatcgcg acgttgaaga aggtcgcgct gcactcgccg
     1861 gccgtcgccc gcgcgagcag cgtcttgccg caacccggcg ggccgtagag caggatgccg
     1921 ccgccgacgc gcttacgaaa gcgctcgaac aggctcggct tctgaaacgg caggatgatc
     1981 ttgcggtgga tctgcttctt gatgtcgtcg agcccgccga cgcccgagaa gtcgatcgac
     2041 tcgggctcgg gcacgagcag ccggtcggcc tcggcggcga ccgtgtcgtc gttcgagatg
     2101 acgcgcaggc gaggtcggcc ctcggcggac gggatcaccc gcgtgcgcgt ctcgagtcgc
     2161 tcggccagcg ccggatcctc gagcgtcgga ttggcggcga cggcccgctg gtaggccgag
     2221 cgggcccgct cgttctggcc cagcccgaac cgcgcccgcg cctgcacgag caacgcccgc
     2281 ggctcgtcct cgccggcgag acggagcgca tcctcgtacc ggtcacccgc gagcaggatc
     2341 gccgccgcgg acagccggtc ctcgagcgga gcctcgtccc agctcgcctg cgccaccagc
     2401 gcggcggcct cggcgcgctc tccccgctcc gcgtgcgcga gcgcgaggac caggcacagc
     2461 ttgacgttgt ccggcgatgc ggcgtacgcc gcctcgagcg accgcaacgt ctcgtcgttc
     2521 accatttgcc ctcgactctc ggagcgtccc gggcagaata tgggagcgcg tggcgaacgg
     2581 tcaaccgcgc agtgcgcgac ctataatcgc cgcgtgtcgc gtcctgaacc aagctcccgc
     2641 gggggagccg agatgctgta ccagacttgc gggtgcaagt cccgcggcgg tcggcctgcc
     2701 ggggcgccgc gtagcaggct tcggttcgga ggcgagatcc tagagcgctc aggggatcgt
     2761 agagtctctg cggaggggag cgaagctgtg ggccgaatag cggagtggta ggtcaaccag
     2821 acggtccgct accctggagg cgtgcacgca tgagaagacg attcgtaagc cgtgtgcggg
     2881 aaattcgcac gcacggtttg aaagggggat tcagcaagcc gggcccgcaa gggcaccgcg
     2941 cctgattcct accaatggac tggacaccgg agcggttcgc cgagctcgac cgcctgttcc
     3001 aacaagcgct cgagctcgag cccgccgcgc gcgaggcgtt cgtcgctcgc cagcagcagc
     3061 gctcccccga gaccggccgg cgactgcgcg agatgctgcg ggccgacggt cgcaccggtg
     3121 cctcgatcga cgaaatcgtg gccgccgtcg cagacgcctt cccgcccgag cgcgaccggc
     3181 tcgtcggcga gcgcttcggg ccgtaccgcg ccgagaggct gctcgggcgc ggcgggatgg
     3241 gcgccgtcta tctcggcgag cggatcgagg gcgacttcgc gcaaacggcg gcgatcaaga
     3301 ccgtgcgttt cggattggag tccccccagc tgctcgaccg gtttcgccga gagcgcgaga
     3361 ttctcgcgca actcgaccat cccggtatcg cccgcctgct cgacggcggc cccggccctc
     3421 acggcctgcc gttcgtggcg atggagtacg tcgacggtct gccgatcacc gagtacgtcc
     3481 ggcgcgagaa atgcggtctc gtgcggcggc tcgagctgtt cgccgagctg tgcgatgccg
     3541 tcgccttcgt ccaccggaac tcgatcgtcc atcgcgacat caaagccggc aacgtgctcg
     3601 tcacacgcga gggtcgcgtc aagctgctcg acttcggcat cgccaagctc gacgacgcct
     3661 tcgatctcgc cggcttgaca cgaaccgagc agcgccccat gacgcccgag tacgcgagcc
     3721 ccgagcaggt actcaaccag ccgatcacca ccgcgtcgga catctactcg ctcggcgtcc
     3781 tgctctacga gctgttgacc gacgtccggc cggtgcggct gacctcgcgc gaaccctacg
     3841 cgatggccaa ggcgatcgtc gaccagatcc ccgcgctccc cagccaggcc gcgtcgccgc
     3901 tgccgccgtg gcagcgccgg ctgcgcggcg atctcgatcg gatcgtgatg acggcgctgc
     3961 gcaaggagcc cgagcggcgg tatgcttcgg tcaccgcgct ggccgacgac gtccggcgct
     4021 acctcgccgg gcggccggtc tcggcgaccg cggacgtttg gcactaccgt ctgcgcaagt
     4081 ggctcggccg ccatccggtg gcgggcgccg tcgcggtcat ctttctggtg acggtggg
//
LOCUS       DASPGN010000599         6529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_465388, whole genome shotgun sequence.
ACCESSION   DASPGN010000599 DASPGN010000000
VERSION     DASPGN010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465388"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..1301
                     /locus_tag="VD788_17390"
     CDS             180..1301
                     /locus_tag="VD788_17390"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXV78088.1"
                     /translation="MRKLRACQALAASMTVAILLTGSTLAQESSTRPPTDLKLVGDHW
                     TAWDPPEAGPDAYIIEKGDTLWDLAGRWLDDPFLWPQIWDENRYILDSHWIYPGDPLV
                     IPGRPTVVPEEPETTEMEPPATGEEAETGEGEETVAETTPAPPPMQLVAGPSDVYCSG
                     YIDPEHEYSTLWVGGSEQPEQRTLGEGDVIYISQGSNQGIRAGDEFGVVRKRQIVAHP
                     TTGQEMGAFIQRLGRLRVMLTQEDTSTAVIDMSCQEIYFSDELVPWETIPIPERRSLP
                     EFSRYDVTPSGGPTGVIVALPEELINVAAGHVVQTDLGLASGVKPGDVLTLYRWQPDL
                     PRLQLGQAIVLTVQQTSSTAVVHSSVAEMAVGDGVEIVN"
     gene            complement(1312..3555)
                     /gene="pnp"
                     /locus_tag="VD788_17395"
     CDS             complement(1312..3555)
                     /gene="pnp"
                     /locus_tag="VD788_17395"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXV78089.1"
                     /translation="MITRKEIEIGGKPLIVESGRLAKQADGAVTVRYGDTVVLVTAVS
                     SATPREGVDFLPLTVDYREATYAAGKIPGGFFKREGRPNEKEVLTSRMIDRPLRPLFP
                     AGWNCETQVIGMLLSSDQENDSDVLAITGASFALGISNIPFPIPIAAVRVGLSESGEY
                     LINPTFRELETSRLNLVVAGSEQAVVMVEAGARECTEEQMLDGILRGHDAIRKIVAVQ
                     HEMIAEIGRPAREAPARVEPAGLAERLTAQWKDRLADAMRIKGKLTSYAAVDELKNEM
                     LEQFDDDQLDQRTFAKRFWHELQDVVLREEVLRKDLRLDGRRFDEIRDLSCDVGVLPR
                     THGSALFTRGETQALVTVTLGTSADAQRLDFVEGESTRRFILHYNFPPFSVGEARFLR
                     GPGRREIGHGALAERSLSPLVPGEDVWPYTLRVVSDILESNGSSSMATICGASLALMD
                     AGVPLRSAVAGIAMGLVKDGDRAVVLTDIAGAEDHHGDMDFKVAGTDKGITGLQMDIK
                     ITGITREIFAQALEQARRARLAILDRMNASISTAREAISRFAPRIITITINKDKIRDV
                     IGPGGKMIRSITERTGCKIEINDDGRIDIASTDENAARQAVEIIKELTAEAELGKTYV
                     GKVVRVVNFGAFVEILPGVEGLLHISEIANRRIGEVRDVLNEGDETVVKVIDIDAQGR
                     VRLSRRAALPADDPDAGPSSESGGGRGGGDRERSDRHRDGGGPRRRGGRDRGERQVAR
                     GRNQGTD"
     gene            complement(3613..3882)
                     /gene="rpsO"
                     /locus_tag="VD788_17400"
     CDS             complement(3613..3882)
                     /gene="rpsO"
                     /locus_tag="VD788_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXV78090.1"
                     /translation="MSLSRDHKEQLIRDFRTHESDTGSPEVQIAILTQRIQYLTDHFK
                     LHAKDHHSRQGLLKMVGKRRRLLDYLRQKNNKRYQEIIGRLGLRK"
     gene            complement(3986..4909)
                     /gene="truB"
                     /locus_tag="VD788_17405"
     CDS             complement(3986..4909)
                     /gene="truB"
                     /locus_tag="VD788_17405"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXV78091.1"
                     /translation="MSAAWDGLLLVDKPSGPTSHDVVDRIRELTGQRRIGHAGTLDPL
                     ATGLLPLVLGRATRLVRFMPHSPKFYQGLLRLGVVTRSDDCTGEIVARHEGPLPEADE
                     VVRAAAVRLGRQLQSPPAVSARKVGGERMYRLARRGVVALAEPRLVEVTRFDVAPCVG
                     STREFRFEAEVSAGTYLRSLVRDLGASLGCGATLVSLRRTAIGSMRPSIELRFDSDPP
                     PVGELRRALIPVERIPLVPPPVRLSSSEDADRFRSGVALAGERGDPWPEGCCRVLGPS
                     GELLGVAEVAAGRLHPRVVLAATRGAGLPGG"
     gene            complement(4906..5271)
                     /gene="rbfA"
                     /locus_tag="VD788_17410"
     CDS             complement(4906..5271)
                     /gene="rbfA"
                     /locus_tag="VD788_17410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXV78092.1"
                     /translation="MSVARNRRVADRIRDELSRILREEVRDPHVGFATVTGVELSPDL
                     RHARVFVSALDDQLEETMLALDRAASFIRRSLARRGGLRFTPSLQFVADRSAATGSRV
                     DRLLQGLEPTGLDDEEGTR"
     gene            complement(5268..5597)
                     /locus_tag="VD788_17415"
     CDS             complement(5268..5597)
                     /locus_tag="VD788_17415"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HXV78093.1"
                     /translation="MTIGIYTFELHLPQARSLKDKRQVVKRLKDRLRSRFNVAVAELP
                     EHADRWQRTGLAVVSIAGNRDTLSALFETIQREAAQQTPGHVIGTGTEFIAAADGGPS
                     GWGERWE"
     gene            complement(5629..>6529)
                     /locus_tag="VD788_17420"
     CDS             complement(5629..>6529)
                     /locus_tag="VD788_17420"
                     /inference="COORDINATES: protein motif:HMM:NF023413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HXV78094.1"
                     /translation="KPGDPFIAGSAYGKVRAMNDDKRNRLDKAGPATPVEVMGFADEP
                     LAGDAFQAVVDEARARQIASFRQDKLRERQQAASARRSLQSLAEDIAAGEIKELPVLL
                     KADVQGSLEALKQALGELPSAKVRVTILRSSIGAISQADVLLAAASDAIVLGFNVRPD
                     KTATDVAKEEKVELRMYTVIYDLIDDIKKAMVGLLEPTLREVVLGQAEVRQLFRVPKV
                     GTIAGCYITDGKVTRNSEVRLLRDNVVIHTGKIASLRRFKDDVSEVKQGYECGIGIAG
                     YNDLKDGDVIESFVMEKVMAESL"
BASE COUNT         1045 a         2205 c         2175 g         1104 t
ORIGIN      
        1 agtcgcgact cgatttctac cgcctcccga cggcttgaat tccggctttt ccggattata
       61 tttggccgcg atgcggtcga ttccgggatg gtgggtcgta agccgatatg ccttgacttg
      121 aatgccggtc ctccaggaac atgccttccg gtaattcagg ccgctgagga gagcgctcga
      181 tgaggaaact acgcgcgtgt caagcgctgg ccgcgagcat gacggtcgcc attctgctga
      241 ccggatccac gctggcgcag gagagctcga ctcgaccgcc gaccgatctc aagctggtcg
      301 gcgaccactg gaccgcgtgg gacccaccgg aggccggacc ggacgcctac atcatcgaga
      361 agggcgacac gctgtgggac ctggcgggcc gctggttgga cgacccgttc ctctggcctc
      421 agatttggga cgagaaccgc tacatcctcg acagccattg gatctacccc ggggacccgc
      481 tcgtcatccc cggccggccg acggtcgtgc cggaggagcc cgaaaccacc gagatggagc
      541 ccccggccac cggggaagaa gcggagacgg gcgaagggga agaaacggtc gccgagacca
      601 ctcccgcgcc gccgccgatg cagctcgtcg ccgggccgtc cgacgtctat tgctctgggt
      661 acatcgatcc cgagcacgaa tactcgaccc tgtgggtcgg cgggtccgag caacccgagc
      721 agcggacatt gggtgaaggc gacgtgatct acatctctca gggcagcaac caggggatcc
      781 gcgccggcga cgagttcggc gtcgttcgca agcgtcagat cgtcgcgcat ccgacgaccg
      841 gtcaggagat gggggcattc atccagcggc tgggcaggct tcgcgtgatg ctgacccagg
      901 aagacacctc gaccgcggtg atcgacatgt cctgccagga gatctacttc agcgacgagc
      961 tggttccgtg ggagacgatt ccgatccccg agcggcggtc tcttcccgag ttcagtcgtt
     1021 acgacgtcac gccgtcgggc ggcccgaccg gggtgatcgt cgcgcttccc gaagagctga
     1081 tcaacgtggc ggcgggccac gtcgtccaaa ccgatctcgg gttggcgagc ggcgtcaagc
     1141 cgggcgacgt gctgacgctc tatcgctggc agccggacct gccgcggctc cagttgggtc
     1201 aggcgatcgt gctgaccgtg cagcaaacga gctcgacggc ggtcgtccac agctcggtgg
     1261 ccgaaatggc cgtgggcgac ggggtcgaaa tcgtcaactg agctccgtct ctcaatcggt
     1321 gccctgattc cttccgcgag cgacctggcg ctcaccacgg tcccgcccgc cgcgacgtcg
     1381 gggcccgccg ccgtcccggt gtcggtcgga ccgctcgcgg tcgccgccgc cgcgaccccc
     1441 gccggattca ctgctcggcc ccgcgtcggg atcgtcggcg ggcagggccg cgcgccgcga
     1501 tagccggacg cgcccttggg cgtcgatgtc gatcaccttg accacggtct cgtccccctc
     1561 gttgaggaca tcgcgtacct cgccgattcg ccggtttgcg atctccgaga tgtggagcaa
     1621 gccttcgacg ccgggcagga tctcgacgaa tgcgccgaag ttgacgacgc ggacgacctt
     1681 cccgacgtag gtcttgccca gctcggcctc ggcggtcagc tccttgatga tctcgaccgc
     1741 ctggcgcgcg gcgttctcgt cggtcgaggc gatgtcgatc cggccgtcgt cgttgatctc
     1801 gatcttgcag cccgtgcgct cggtgatcga tcggatcatc ttgccgccgg ggccgatgac
     1861 gtcgcgaatc ttgtctttgt tgatcgtgat cgtgatgatg cgcggggcga aacgggaaat
     1921 cgcttcgcgt gccgtcgaga tcgaggcgtt catccggtcg agaatggcga ggcgcgctcg
     1981 gcgggcctgc tcgagcgctt gggcgaagat ctcgcgcgtg atgccggtga tcttgatgtc
     2041 catctgcaga ccggtgatgc ccttgtccgt tcccgccact ttgaagtcca tgtcgccgtg
     2101 gtggtcttcg gcgcccgcga tatcggtcag cacgacagcg cggtctccgt ccttgaccag
     2161 gcccatcgcg atcccggcga cggccgatct cagcgggacg ccggcatcca tcagcgcgag
     2221 cgacgcaccg cagatcgtcg ccatcgatga cgagccgttc gactcgagaa tgtcggagac
     2281 gacgcgcagc gtgtagggcc agacgtcctc gccggggacc agcggtgaga gggacctctc
     2341 ggcgagtgcg ccgtgaccga tctcgcgccg gcccggaccg cgcaggaatc gtgcttcacc
     2401 gaccgagaac ggcggaaagt tgtagtgcag aatgaagcgc cgcgtgctct cgccctcgac
     2461 gaagtcgagc cgctgcgcgt cggccgaggt acccagtgtg accgtcacca gcgcctgcgt
     2521 ctcgccgcgc gtgaacagcg ccgagccgtg ggtgcggggc aggacgccga cgtcgcagga
     2581 gaggtcgcgg atctcgtcga accgccgccc gtccagccgg aggtccttcc gcaacacctc
     2641 ctcgcgcagg acgacgtcct gcaactcgtg ccagaaccgc ttggcgaagg tcctctggtc
     2701 gagctggtcg tcgtcgaact gctcgagcat ctcgttcttc agctcgtcga cggcggcgta
     2761 gctcgtgagc ttgcccttga ttcgcatcgc gtcggccagc cggtccttcc attgagcggt
     2821 gagccgctcg gccaagccgg cgggctcgac acgggcgggc gcttcgcggg ccggcctgcc
     2881 gatctccgcg atcatctcgt gctgaaccgc cacgatcttg cggatcgcgt cgtgaccccg
     2941 gaggatgccg tcgagcatct gctcttcggt gcactcgcgc gcgccggcct cgaccatcac
     3001 caccgcttgt tcggagccgg cgacgacgag gttcagcctc gaggtctcca actctcggaa
     3061 cgtcggattg atcaagtact cgccgctctc cgacagcccg acgcgaacgg cggcgatcgg
     3121 aatcgggaag gggatattcg agatcccgag cgcgaacgac gcaccggtga tcgccagcac
     3181 gtcggagtcg ttctcttggt cggaggagag cagcatgccg atgacctgcg tctcgcagtt
     3241 ccagccagcc ggaaacagcg gtcgcagcgg cctgtcgatc atccgcgagg tcagcacctc
     3301 tttttcgttg ggacggcctt cgcgcttgaa gaacccgccg gggatcttgc cggccgcgta
     3361 ggtcgcctcg cggtagtcga cggtcaacgg cagaaagtcg accccttccc gaggggtggc
     3421 cgacgagacg gcggtcacca gcacgacagt gtcgccgtag cggacggtga cggctccgtc
     3481 ggcctgcttc gccaacctgc cgctctcgac gatcagcggt ttgccgccga tttcgatttc
     3541 cttgcgtgtg atcaagatgg aaacctcctg ggctgctcgc tactcgctcg aatcggtggg
     3601 gcggggctcg gcctacttac gcaggccgag acgaccgatg atctcttggt aacgcttgtt
     3661 gttcttttgc ctgagatagt cgagcaggcg gcgacgcttg ccgaccatct tgagcaagcc
     3721 ttggcgcgag tggtggtcct tggcgtggag cttgaaatgg tcggtcaggt actggatgcg
     3781 ctgcgtgagg atcgcgatct ggacttcggg ggagccggtg tccgactcgt gcgtgcgaaa
     3841 atcgcgaatc agctgttcct tgtgatccct cgaaagagac acggccggtg atcctccaac
     3901 cccgccgaca atcgcgaacg ggtcagataa agtcttgttt ttctgctagt tgtcgccagg
     3961 acaaccgcca gacgttagca caggctcatc ccccgggcaa gcccgccccg cgcgtcgccg
     4021 ccagcacgac ccgcgggtgg aggcgtcccg ctgccacctc ggcgacgccg agcagctcgc
     4081 ccgaggggcc gagcacgcgg cagcaaccct ccggccacgg gtcgccgcgc tcaccggcca
     4141 acgcgacccc cgagcgaaag cgatcggcgt cctccgacga gctcagtcgg accggcggag
     4201 gtacgagcgg gatgcgttcg accgggatca gcgcacggcg cagctcgccg accggaggcg
     4261 ggtcggagtc gaaacgcagt tcgatcgacg gccgcatcga tccgatcgcc gtgcgacgca
     4321 acgagaccag cgtcgcgccg caaccgagac tcgcgccgag atcccgcacg agcgaccgga
     4381 ggtaggtccc ggccgagacc tcggcctcga aacggaattc gcgggtcgat ccgacgcatg
     4441 gcgcgacgtc gaagcgggtc acctcgacca ggcgaggctc ggccagcgcg acgacgccgc
     4501 ggcgtgccag gcggtacatc cgctcccctc cgaccttgcg cgccgacacc gccggcgggc
     4561 tctgcagctg ccggccgagc cgaacggccg cggcgcgcac cacctcgtca gcctcgggca
     4621 gcggtccctc gtgccgcgcc acgatctcgc cggtgcagtc gtccgaccgc gtcacgacgc
     4681 cgagccggag caggccctgg tagaacttcg gcgaatgcgg catgaagcga acgagacgcg
     4741 tcgcccgtcc gaggaccagt ggaagcagcc cggtcgcgag cggatcgagc gttcccgcgt
     4801 gaccgatccg tcgttgtccg gtcaactcgc ggattcggtc gacgacgtcg tggctggtcg
     4861 gtcccgacgg cttgtcgacg agcagcaggc cgtcccacgc cgcgctcacc gcgtaccttc
     4921 ctcgtcgtcg aggccggtcg gctcgagccc ctggagcaag cgatcgacgc gggaaccggt
     4981 cgcggcggac cgatccgcga cgaactgcag cgacggagtg aagcgcaggc cgccgcgcct
     5041 ggccagcgat cggcggatga acgacgcggc acggtccagc gccagcatcg tttcctcgag
     5101 ctggtcgtcg agcgcactga cgaagacacg cgcgtgacgc agatcgggcg agagctcgac
     5161 gccggtcacc gtcgcaaagc cgacgtgcgg atcgcggacc tcctcgcgca ggatgcgcga
     5221 aagctcgtcg cggattcgat cggcgacgcg cctgttccgc gcgacgctca ttcccaccgc
     5281 tcgccccagc cggacgggcc cccgtcggcg gcggcgatga actccgtgcc ggtgccgatc
     5341 acgtgcccgg gggtctgctg cgccgcctcg cgctgaatcg tctcgaacag cgcgctcagc
     5401 gtatcgcggt tcccggcgat cgacaccacc gcgagtccgg tgcgttgcca gcggtcggcg
     5461 tgttcgggca attcggcgac cgcgacgttg aaccgcgaac gcaaccggtc cttcaatcgc
     5521 ttgacaacct ggcgcttgtc cttcagcgat cgagcctgtg gcaggtgcag ctcgaaggtg
     5581 tagatgccga tcgtcacgac gcgccctcgc aagggcggcg gacggcgatc agaggctctc
     5641 cgccatgacc ttctccatga cgaagctctc gatcacgtcg ccgtccttga ggtcgttgta
     5701 gccggcgatg ccgatcccgc actcgtagcc ctgcttgact tcggaaacgt cgtccttgaa
     5761 tcgccgcagc gaggcgattt tgcccgtgtg gatcacgacg ttgtcacgca acagcctgac
     5821 ttcggagttg cgagtgacct tgccgtcggt gatgtagcag ccggcgatcg taccgacctt
     5881 cggcacgcgg aacaactgcc ggacctcggc ttggccgagg acgacctctc tcagtgtcgg
     5941 ctcgagcaag ccgaccatcg ccttcttgat gtcgtcgatc aggtcgtaga tcacggtgta
     6001 catccgcaac tcgaccttct cctccttcgc cacgtcggtc gcggtcttgt cgggacggac
     6061 gttgaagccg aggacgatcg cgtcggaggc ggcagccaac agcacgtcgg cttgcgaaat
     6121 cgcgccgatc gacgaacgca ggatcgtgac gcggaccttg gccgacggca gctcgccgag
     6181 tgcctgcttg agcgcctcga gcgacccttg gacatcggcc ttgagcagca ccgggagctc
     6241 cttgatctca ccggcggcga tgtcttcggc cagactctgc agcgagcggc gcgccgaggc
     6301 cgcctgctgg cgttcgcgca gcttgtcctg gcggaacgag gcgatctgtc gggcgcgggc
     6361 ctcgtcgacg acggcctgga acgcgtcacc cgccagcggt tcgtcggcga agcccatcac
     6421 ttcgaccggg gtcgccggcc cggccttgtc cagccggttg cgcttgtcgt cgttcatcgc
     6481 gcggaccttg ccgtaggccg acccggcgat gaacggatcg cccggcttg
//
LOCUS       DASPGN010000600         8039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_113400, whole genome shotgun sequence.
ACCESSION   DASPGN010000600 DASPGN010000000
VERSION     DASPGN010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113400"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VD788_17425"
     CDS             <1..195
                     /locus_tag="VD788_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78095.1"
                     /translation="SDPRWAMGDERGLILLLARKGDLWGEQTDTPVRWGVFPTDATVR
                     GPKPGVLEFAGHPYRLRVAG"
     gene            complement(198..1715)
                     /locus_tag="VD788_17430"
     CDS             complement(198..1715)
                     /locus_tag="VD788_17430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV78096.1"
                     /translation="MARSTTGGIYAVPVGVALLKLLVHLPVLHRYGFHHDELYFIACG
                     RHPAFGYVDHAPLVPWLARLATALFGESVAGLRIFATLAGAVTIVLVGVLVARLGGGR
                     FAQLLACVAWFVAPVALRTGNMLAIPSFEPLFWLAGSLVLVRILNEDDPRGWPWLGLL
                     AGAGLMNKHSMLFFGFGVALATVVTPRLRRQLLTPWPWIGAAVALLVFLPNVIWQVQH
                     GWPTAGFLRALNENVMAGISKLQFVAGQILYLNPFAAFVWIWGLVFLFARAGRPWRAL
                     GVIWLAVFLLLLATDSKIYYLAPAYPAIVAAGGVAIEAWVAACERRRRLKPVLVALPV
                     LGGLLFAPLALPFLSIRETDRFARTITFGAMDNVYELTGDLHGMFGWPERVEAVAEVW
                     RAVPPAERPRTVLFAAGYGNAGAVDFLGDAHGLPDAVSLAENYWMWGLPEGPIDTVIA
                     LGFSAETLDRVFETVEVVREVDLDDVNPDSTPFRIAICREPKLPLAELWARNRPW"
     gene            1807..2817
                     /locus_tag="VD788_17435"
     CDS             1807..2817
                     /locus_tag="VD788_17435"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXV78097.1"
                     /translation="MLTVRQVPAAVGICLLMAGCAGPADGPAGDTLWSNWGEDEWYCR
                     TERRDVELYVRAIGIGEPVLVLHGGWGGEHSYLTDLVRPHVRERRFVLYDQRGSLRSP
                     APAEAISLDAMLADIERIRGQLGRQTIDIVAHSMGTYLAMKYLESHPDRVGRLVLLAP
                     HRPASDLPLLADGTRLDYDDAPATNDWVYREATWAEMREEGVAPQEGVPDTQMSDLAW
                     TKQWRISFAGANLYDVSKWRQVRGGHVFYNPNVYAAIRDSVPQEWNFIPALSAHEKGV
                     HVIIGDHDFVDFGARFWRWAESQAPTVELRLIERAGHNSWIDDPQAVAAALRAALGIG
                     NP"
     gene            complement(2922..3992)
                     /locus_tag="VD788_17440"
     CDS             complement(2922..3992)
                     /locus_tag="VD788_17440"
                     /inference="COORDINATES: protein motif:HMM:NF025587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HXV78098.1"
                     /translation="MSNRKDDGVRAVNRDFSRYDQVDKSHTYRLAGGTPDEYDESFVL
                     PICDIGQLFEGGADGRQSFAEQLGGSLERIGFACIHGHGIDPVLWNQAEARVIDLFTR
                     PSLDDKLRFRAARHGSVNQGYFPIKETSEIHPDLVEGWVFCRRAFDLDGDPGYDASRF
                     WALPEHEPHFRRIVEANERLILPLTQAIFRYLGCDPHLYDEKLTGTNIGLRLNYYPPI
                     RQRDEASGAGRLLGHEDVDLFTLLPAPRVEGLEAWNRASGRWVRLDAPPGSLILNTGD
                     YMQRITNDRLRSTTHRVSQPRDESQRGTTRVSIPMAVYLAEDELLEVLPGLGRPRYEP
                     IMAITFHTRSTSKFYGDGYAVD"
     gene            complement(4005..5306)
                     /locus_tag="VD788_17445"
     CDS             complement(4005..5306)
                     /locus_tag="VD788_17445"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HXV78099.1"
                     /translation="MTAPVRFASRLTTILTMVGVAVGLGNVWRFPYMMGSYGGSAFLF
                     VYLAFVLLLGIPAVIGEWALGRETRRGTLGALDAAFGRAGRPLGWILVGALAVANSYY
                     LVVIGNVVYTMLFSVAVGFRDETLAAYRSGLTGAAIQASITLAALASALAVLALGFNR
                     GAERLSRWFVPISGGMLIYLIAHSLALPGAIGALARFLRPDFAALTPTHVFAAMGQAF
                     YSLSLGGTMLVVYGSYLREQENIRTAAIATALGDAGTALLAALFLVPTTLVLGLELTA
                     GPQLVFDTLPRLFALVSGGRVAGTLFLAALAMVAYLSTLAALEVLLGALRDTLGLSTR
                     TALSVLGATQAGMLLPSVLWPRIIGTLDLVVGSGMQLAGSGVALIALGWGLGRATLLT
                     QVFGAADASGFWPQSYLLWVRWVVPFALAVTLALYLYDSLF"
     gene            complement(5316..5792)
                     /locus_tag="VD788_17450"
     CDS             complement(5316..5792)
                     /locus_tag="VD788_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXV78100.1"
                     /translation="MANQPFDTDEEIDLRAPVEGDGGILTIRAGETCRSWNGIRYEVG
                     MSAKNVGSTQLSMNVATIPPGGVARAHVHVGFELMLYILEGRVRHEYGPGCRRVLENG
                     PGDLIYIEPDVPHEVFNVSDSEPVRAVVARSDASEWSNIVRYDRETERLIPDGERS"
     gene            5942..6595
                     /locus_tag="VD788_17455"
     CDS             5942..6595
                     /locus_tag="VD788_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78101.1"
                     /translation="MWLRSWAMLVFVATSVGCLAGKTTSTVYIETDDSISWTILETDV
                     RSNADEAAMRERQEREFIRAAKRGEHDTVGGFDLLAPDEVDTVILRDERPFSVATTAR
                     FRSLERLARTVLDVLEVGGQVRIETTDELTRLEVEIDLPEADRQTAAGDEGSAAGESW
                     FEELEIVVADGRFVESRGFVQAADDRVRFAASDELLRMAAAADGRLVLSLAWTHDAR"
     gene            6665..>8039
                     /locus_tag="VD788_17460"
     CDS             6665..>8039
                     /locus_tag="VD788_17460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXV78102.1"
                     /translation="MRGSAVSRRVAVWLFAVGLALRLVHLLTVRDSALFNLLYIDPKL
                     YDEWGLAIAGGRLLGEAPFFLDPLYPYVIGAIYAVTGRSLVAVAVVQVVLGACVAPLT
                     YDAARRWFDPAVAGLAGAVAVVYPPSIYYSVVLLKPGIAIFLVALALWLLARALGDGR
                     TPAWLATGFAFGLTVLMRTNLLLVVPLIALAVALERGAGGDSAGRGLAGWRAAVALAA
                     GCLTALLPATVHNLAAGGEPILATTNWGQIFYIGNNEHNPTGRFEELPFVRSNPAFEQ
                     IDFKNEAERRAGRELSHGATSRFWFGEALDWTLAHPAAWAGLQWSKLRIYWGGYETPA
                     SLDYNLYSRHAPLLGLHLPGFGLLGPFGLLGAVLALGRPGWPRLLALWVGAGSLSVVA
                     FFVLTRFRMVVVPALYVLAAAAVVELWRAYRRPADEHGWVRPLWLTTLLLVFFAFVNL
                     PVRATADS"
BASE COUNT         1267 a         2737 c         2849 g         1186 t
ORIGIN      
        1 tcggatcctc gctgggcgat gggtgacgag cgcggtctga tcctgctcct cgcccgcaag
       61 ggcgacctgt ggggcgagca gacggatacg ccggtgcgct ggggcgtctt cccgacggac
      121 gcgacggtcc gcggcccgaa gcccggcgtc ctcgagtttg ccggccatcc gtacaggctg
      181 cgcgtcgccg gctaggtcta ccaggggcgg ttgcgggccc acagctcggc gagcggcagc
      241 ttgggctcgc ggcagatcgc gatacgaaac ggtgtcgagt cggggttgac gtcgtcgagg
      301 tccacctccc gtaccacctc cacggtctcg aaaacgcgat cgagcgtctc ggcggagaac
      361 cccagcgcga tgaccgtgtc gatcgggcct tcgggcagcc cccacatcca gtagttctcg
      421 gcgagactca ccgcgtcggg cagaccatgg gcgtcgccga ggaagtcgac cgcgccggcg
      481 ttgccgtaac cggcggcgaa cagcaccgtg cgtggacgct cggccggcgg gactgcccgc
      541 cacacctcgg ccacggcctc gacgcgctcc ggccaaccga acatcccgtg caggtcgccg
      601 gtcagctcgt agacgttgtc catcgcaccg aaggtgatcg tccgcgcgaa gcgatccgtc
      661 tctcggatcg acaggaacgg cagcgccagc ggggcgaaca gcagtccgcc gaggacgggc
      721 agcgcgacga gcaccggctt caaccggcgg cgccgctcgc atgcggcgac ccaagcctcg
      781 atcgccacgc cgcccgcggc cacgatcgcc ggataggccg gggccaggta gtagatcttg
      841 ctgtccgtcg ccagcagcaa caggaacacc gcgagccaga tcacgcccag cgcgcgccac
      901 ggcctgcccg ccctcgcgaa caggaagacc aggccccaga tccagacgaa cgcggcgaac
      961 gggttgaggt agaggatctg tccggcgacg aactgcagct tcgagatccc cgccatcacg
     1021 ttctcgttca gcgcgcgcag gaagccggcg gtcggccagc cgtgctgcac ctgccagatc
     1081 acgttaggca ggaacacgag gagcgccacc gccgcgccga tccacggcca cggcgtgagt
     1141 agctgccggc gcagccgcgg cgtcacgacc gtcgcgagcg cgacgccgaa gccgaagaac
     1201 aacatcgagt gcttgttcat cagaccggcg cccgcgagca gccccaacca cggccacccg
     1261 cgcgggtcgt cctcgttcag gatgcgcacc agcacgagcg acccggcgag ccagaacagc
     1321 ggctcgaacg acggaatggc cagcatgttc cccgtgcgca gcgccaccgg cgcgacgaac
     1381 caggcgacgc aggccagcag ttgcgcgaag cggcctccgc ccaggcgagc gaccagcacg
     1441 ccgaccagga cgatcgtcac cgctccggcg agcgtggcga agatgcgcag gccggcgacc
     1501 gattcgccga acagcgcggt cgccagacgc gcgagccacg gcaccagcgg ggcgtggtcg
     1561 acgtagccga aagccgggtg acggccgcac gcgatgaaat agagctcgtc gtggtggaag
     1621 ccgtaacgat gcagcaccgg caggtggacg agcagcttga gcagcgccac gcccaccggt
     1681 accgcgtaga tcccgcccgt tgtcgatcgc gccatacgtc ccggtctcaa tggccggcgg
     1741 acattttcgc aacgtccgcc ggcagcgtcg aatcgtacta cgaagtacca cagcggaggg
     1801 cgcgacatgc tgacggttcg acaggtgccg gcggccgttg gaatctgctt gctgatggcg
     1861 ggctgcgctg gtccggccga cgggccggcg ggcgataccc tgtggagcaa ctggggcgag
     1921 gacgagtggt actgccgcac cgagcggcgc gacgtcgagc tctacgtgcg ggcgatcggc
     1981 atcggcgagc ccgtgctggt gctccacggg ggctggggcg gcgagcattc gtatctgacc
     2041 gatctcgtcc ggccgcacgt gcgggagcgg cgcttcgtgc tctacgacca gcgcggatcg
     2101 ttgcgttcgc cggcaccggc cgaggcgatc agcctcgacg cgatgctcgc cgacatcgag
     2161 cgcatccgcg ggcagctcgg tcgccagacg atcgacatcg tggcgcactc gatgggcacc
     2221 tacctcgcga tgaagtacct cgagtcgcat ccggatcggg tgggccggct ggtgctgctc
     2281 gcgccacacc ggccagcgag cgatctgccg ctcttggcgg acggaacccg tctcgactat
     2341 gacgacgcgc ccgcgaccaa tgactgggtc tatcgcgagg cgacctgggc cgagatgcgc
     2401 gaggaaggcg tcgcgccgca ggagggcgtg cccgacaccc agatgtccga cctggcctgg
     2461 accaagcagt ggcgcatctc attcgccggg gcgaatctct acgacgtcag caagtggcga
     2521 caggtccgtg gcgggcacgt cttctacaac ccgaacgtgt atgccgcgat ccgcgactcc
     2581 gtcccgcagg aatggaactt catcccggcg ctgtcggcgc acgagaaggg ggtccacgtg
     2641 atcatcggcg accacgactt cgtcgacttc ggcgccaggt tctggcgctg ggcggagagc
     2701 caggcgccga cggtcgagct gcggctgatc gagcgcgcgg gccataacag ctggatcgac
     2761 gacccgcagg cggtggccgc ggcgctgcgc gcggcgctgg ggatcggcaa cccgtgaccc
     2821 ggaccggggc ggtccgccga gtgtctcacc gcgctgccga ccgataggcc tgctcgagcg
     2881 ccgcgatgtc gagttgacca tcgacaacag acggcgggcg gtcagtcgac ggcgtaaccg
     2941 tcaccgtaga acttgctcgt cgagcgggtg tggaacgtga tcgccatgat cggctcgtag
     3001 cgcggtcgac cgaggccggg cagcacttcg agcagctcgt cctcggcgag gtagaccgcc
     3061 atcgggatcg acacgcgtgt cgtgccgcgc tggctttcgt cgcgcggctg cgagacgcga
     3121 tgcgtggtcg agcgcagtcg gtcgttggtg atgcgttgca tgtagtcgcc ggtgttgagg
     3181 atcaacgacc ccggcggcgc gtcgagtcgc acccacctgc cgctcgcgcg attccacgcc
     3241 tcgagcccct cgacgcgcgg tgccgggagc agggtgaaca gatcgacgtc ttcgtgcccg
     3301 agcaaccgtc ccgcgcccga cgcctcgtcg cgctggcgga tcggcggata gtagttgagt
     3361 cgcaagccga tgttggtccc ggtcagcttc tcgtcgtaca ggtgcgggtc gcagccgaga
     3421 tagcggaaga tcgcctgcgt gagcggcagg atcaggcgtt cgttggcctc gacgatgcgg
     3481 cggaagtgcg gctcgtgctc cggcagcgcc cagaagcgag acgcatcgta gcccgggtcg
     3541 ccgtcgagat cgaacgcgcg acggcagaag acccagccct cgaccaggtc ggggtggatc
     3601 tcgctcgttt ccttgatcgg aaagtaaccc tggttgaccg agccgtgacg cgcggcgcgg
     3661 aagcgcagct tgtcgtcgag cgaggggcgg gtgaacaggt cgatcacgcg cgcctcggcc
     3721 tggttccaca acacgggatc gatgccgtga ccgtggatgc aggcgaagcc gatgcgctcg
     3781 agcgagccgc cgagttgttc ggcgaacgac tgccggccgt cggcgccgcc ctcgaacagc
     3841 tgtccgatgt cgcagatcgg caagacgaac gactcgtcgt actcgtccgg cgtgccgccg
     3901 gccaggcggt aggtgtggct cttgtcgacc tggtcgtatc ggctgaaatc gcggttgacg
     3961 gcgcgcacgc cgtcatcttt acgattcgac atggcggcac tcctctagaa caacgagtcg
     4021 tacaagtata aggccagcgt gacggcgagc gcgaacggca cgacccagcg cacccacagc
     4081 aggtagcttt gcggccagaa gcccgaagcg tcggccgcgc cgaacacctg ggtcaagagc
     4141 gtggcgcggc cgaggcccca gccgagcgcg atcaacgcga ccccgcttcc cgcgagctgc
     4201 atgccggagc cgacgaccag gtcgagcgtg ccgatgatcc gtggccagag gacgctcggc
     4261 agcagcatcc cggcctgcgt cgcgccgagc accgacaacg ccgtgcgcgt cgacaggccg
     4321 agcgtgtcgc gcaatgcgcc gagcagcacc tcgagtgcgg cgagcgtcga caggtaggcc
     4381 accatcgcca gcgccgcgag aaacagcgtg ccggcgacac ggccgccgga cacgagcgcg
     4441 aacagccggg gcaacgtgtc gaagaccaac tgcgggccgg cggtcagctc gagacccagc
     4501 accagcgtcg tcggcacgag gaacagtgcc gccaataacg ccgtgcccgc gtcgcccagc
     4561 gcggtggcga tcgccgcggt gcggatgttc tcctgctcgc gcaggtagct gccgtagacc
     4621 accagcatcg tcccgcccag gctgagcgag tagaacgcct ggcccatcgc ggcgaagacg
     4681 tgcgtcggcg tgagcgcggc gaaatcgggg cgcaggaagc gggccagcgc gccgatcgcg
     4741 cccggcagcg ccagcgaatg ggcgatcagg tagatcagca tgccgccgct gatcggcacg
     4801 aaccagcggc tcaagcgctc ggcgccgcgg ttgaaaccga gcgccagcac ggcgagcgcc
     4861 gaggccagcg cggcgagcgt gatcgacgcc tgtattgcgg cgccggtcaa ccccgagcgg
     4921 taggcggcga gcgtctcgtc gcggaagccg acggcgacgg agaacagcat cgtgtagacg
     4981 acgttgccga tcacgaccag gtagtacgag ttggcgacgg cgagcgcgcc gacgaggatc
     5041 catcccagcg ggcggccggc cctgccgaac gctgcgtcga gtgcgccgag cgtaccgcgc
     5101 cgcgtctcgc gcccgagcgc ccactcgccg atcaccgccg ggatgccgag caggaggacg
     5161 aaagcgagat agacgaacag gaaggcgcta ccgccgtagc tgcccatcat gtacgggaag
     5221 cgccagacgt tgcccaggcc gaccgccacg ccgaccatcg tgaggatcgt cgtcagccgc
     5281 gaggcgaagc ggacgggcgc ggtcaagcgc gagccctacg aacgctcccc gtccgggatc
     5341 aaccgctcgg tctcgcggtc gtagcgcacg atgttcgacc actcgctggc gtccgaccgc
     5401 gcgacgacgg cccgcaccgg ctcgctgtcg ctgacgttga acacctcgtg cggcacgtcc
     5461 ggctcgatgt agatcaagtc gcccggcccg ttctcgagca cgcggcggca acccgggccg
     5521 tactcgtggc gcacgcgccc ctcgaggatg tagagcatca gctcgaagcc gacgtgcacg
     5581 tgcgcccggg ccacgccgcc cggcgggatg gtcgcgacgt tcatcgacag ctgggtcgac
     5641 ccgacgttct tggccgacat gccgacctcg tagcggatcc cgttccagct gcggcaggtc
     5701 tccccggcgc ggatcgtcag gatgccgccg tcgccctcga cgggcgcacg gagatcgatc
     5761 tcctcgtcgg tgtcgaacgg ttggttggcc atcggtcgct cctctcgtgg gacagtgtac
     5821 gccgggaacg gccggtcggt gccggtttgt tgctgggtcc gcaccgcgtt cggtccgacc
     5881 ccgccgcgtc gtctttgtcg atgcgggcgg acgagccgcc gcggcagcgg aggtgaagac
     5941 aatgtggtta cgcagttggg cgatgctggt cttcgtggcg acttcggtcg gttgcctggc
     6001 cggaaaaacg acctcgacgg tttacatcga aaccgacgat tcgatcagct ggacgatcct
     6061 cgagaccgac gtccgctcga acgccgacga ggcggcgatg cgcgagcgtc aggaacgcga
     6121 gttcatccgt gcggcgaaac ggggcgagca cgacacggtc ggcggcttcg acctgctcgc
     6181 gcccgacgag gtcgacacgg tgatcctgcg cgacgagcgg ccgttctcgg tcgcgacgac
     6241 ggcgcgcttc cgctcgctcg agcggctggc gcgaacagtg ctcgacgtgc tcgaggtcgg
     6301 cgggcaggtg cggatcgaga cgacggacga gctgacgcgc ttggaagtcg agatcgactt
     6361 gccggaggcg gatcgacaga cagccgcggg cgacgagggt agcgcggccg gcgagagctg
     6421 gttcgaggaa ctcgagatcg tcgtggccga cggccgattc gtcgagtcgc gcgggttcgt
     6481 ccaggccgcc gacgatcgcg tgcgcttcgc cgcgagcgac gagctgctgc ggatggcggc
     6541 cgccgcggac ggccggctgg tgctgtccct cgcttggacc cacgacgcac ggtgaacgca
     6601 ccgggtgagg gacgccgtgc tgtaggatgg agccggcccg tgtaacgaac ggatgggtcg
     6661 gtcattgagg ggtagcgcgg tttcccgtcg cgtcgcggtc tggctgttcg ccgtcggttt
     6721 ggcactgcgg ctcgtgcatc tgctgacggt gcgcgacagc gcgctgttca atctgctcta
     6781 catcgacccg aagctgtacg acgagtgggg gctcgcgatc gccggcggcc gactgctcgg
     6841 cgaggcgccg ttctttctcg atccgctcta cccgtacgtc atcggcgcga tctacgccgt
     6901 caccggccgc agcttggtcg cggtggccgt cgtgcaggtc gtgctcggtg cgtgcgtcgc
     6961 tccgctgacg tacgacgccg cgcggcgctg gttcgaccct gccgtcgcgg gtctggccgg
     7021 ggcggtcgcc gtggtctacc cgccgtcgat ctactactcg gtcgtcctgc tgaagccggg
     7081 aatcgcgatc ttcctggtcg cgctcgcgtt gtggctgctc gcgcgggccc tcggcgacgg
     7141 ccggacgccg gcgtggctcg ccaccggctt cgccttcggc ctgacggtgt tgatgcgaac
     7201 caacctgctc ttggtcgtcc cgctgatcgc gctcgccgtc gcgctcgagc gcggtgcggg
     7261 gggcgattcg gcgggccggg gcctcgccgg ctggcgcgcc gccgtcgcgc tcgcggcggg
     7321 ctgcctgacc gccctgctgc cggcgacggt gcacaacctg gccgcgggcg gcgagccgat
     7381 cctggccacc accaactggg gtcagatctt ctacatcggc aacaacgagc acaacccgac
     7441 cgggcgtttc gaagagctgc cgttcgtccg ctcgaatccc gccttcgagc agatcgactt
     7501 caagaacgag gccgagcgcc gtgccggccg cgagctgagt cacggtgcga cctcgcgctt
     7561 ctggttcggc gaggctctcg attggacgct cgcgcacccc gccgcctggg ccgggctgca
     7621 gtggagcaag ctgcgaatct actggggtgg ttacgaaacg ccggccagtc tcgactacaa
     7681 cctctacagc cgtcacgcgc cgctgctcgg cctgcacctg cccggcttcg gcctgctcgg
     7741 cccgttcggt ttgctcggtg cggtgctcgc gctgggacgc ccgggctggc cgcggctgct
     7801 cgccctgtgg gtcggcgccg gctcgctgtc ggtcgtcgcg ttcttcgtgc tgacccgttt
     7861 ccgtatggtg gtcgtacccg cgctctacgt cctcgcggcc gcggcggtgg tcgagctgtg
     7921 gcgcgcctac cgtcgtccgg ccgacgagca cggctgggtc agaccgctgt ggctgacgac
     7981 gttgctgctc gtcttcttcg ccttcgtcaa cctgccggtg cgggcgacgg ccgacagct
//
LOCUS       DASPGN010000601         6130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1166519, whole genome shotgun sequence.
ACCESSION   DASPGN010000601 DASPGN010000000
VERSION     DASPGN010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166519"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VD788_17465"
     CDS             <1..1128
                     /locus_tag="VD788_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78103.1"
                     /translation="PVRRLLPCGVLGGAALAVAAGAAGEPPTLRFDDAPRSGQFGSVS
                     VSGLPEPALARLAERLENSDWERLLAVRIGEPGAAAAPPTVLGSYEVTGGVLRFRPRF
                     PIAAGLVFHAHFDGARFDDWSGAAAGTRTLTATFRMPEPAERAAPRVVVVYPSATELP
                     ENLLRVYVHFSEPVFPKDVHDQVRLFDAGGEPVELPFVEIEGGLWDPARRRLTLFLHP
                     GRIKRGVAPNTELGPPLRAGESFRLAVGGGFEKPIRALPADREAPDPATWRVGAPAAR
                     GEPLTLDFPEPLDHALLGRLIEVRGPDGATLPGRVTISHGETRWTFTPERPWFAGDHV
                     LRIDPALEDVAGNAVAGAFEHRGTTRSPAPAEPVEIPFRVP"
     gene            complement(1150..1434)
                     /locus_tag="VD788_17470"
     CDS             complement(1150..1434)
                     /locus_tag="VD788_17470"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HXV78104.1"
                     /translation="MCRNIRTLFNFEPPATDDEIRAASLQFVRKLSGFTRPSNANQAA
                     FERAVESVTAVARELLASLVTSASPKDRETEAARARARAGERFTAARGKR"
     gene            1844..5686
                     /locus_tag="VD788_17475"
     CDS             1844..5686
                     /locus_tag="VD788_17475"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV78105.1"
                     /translation="MPYARIACLTLLLAIAPPVSIHAGSDPARMDAGILRVEEHASRA
                     VALPPRATGAGQRLARIDGALYQKSGELYLPVVPDRISVRLADDVKSWDELVARTVAR
                     DPKAYAPLADLVAERTNRLGVVDLRVPEGSDLIPWLDLLETTGEVRYAEVASRGVYLA
                     TQSDPLYPQQYALNNTGQTGGTPGADVDAERAWDLTAGDPSVIVAVLDSGTAIDHEDL
                     AANVWHNDGEIPGNGIDDDGNGFVDDWEGWDFAGNDNDPRSGNEHGTFVTGIVNAAGS
                     NGAGVAGLAGGLGGPGVRAMILSVGSSGPVTDVIDDAILYAADRGARVITLSLSVGET
                     QAINDALDYAYNLRDVFVDCAAGNSGSAVSYPALRPEVMAVGSTDHDDLSSGFSNPGP
                     EVEVSAPGSSVLSTALNNGYTTSSGTSFAAPRVAALAALIYSRNPGLAAADVRQIIID
                     TAEDVGDPGFDERTGHGRINAYEALLIAADSDGVIRLDRAAYACDATVGLTVSDVDLA
                     GSGTIDVTASSETEPAGETVTLTETGAGSGLFRGALAADGGAAQPDGRLQLSDGDTLV
                     AEYVDQDDGSGGTGVTKTATSTADCRAPLVADVAVDGVTTSTATIHWTTDEAADTRVR
                     YGDDLPPSQLEQSAGFVYQHQVALSGLDQCTIYKFAVESSDDVGNLGIDDNDGTYFVF
                     ETLVQVPGVGAVPCHQALLAFDDASPAGCEEGVGLSLLDSDLDTDSGQVESVQVRLAS
                     TREPEGEWVTLTEINPQSSRFEGQGMLDRGPPVAGDGLLAVADGDFVTATYHDPDDGQ
                     GRPWIATASKTADCEAPRIGDVRVTEIAATRAVIEWTTDEPGTSRVEFGPTAALGNSV
                     EDPTLRTDHRVVISVFDACERVHFRVSSVDTHGETRVADAAGVPFSFNVNEIPGLVFH
                     ETFETDTGWTLPDDWERGTPQGLGSGAGDPTAAWSGLSVIGNDLSGLGAFAGDYEPVT
                     SSSALSPVFSTRQQRGLELIVHRKLGVTSTDEAGIYVVTSGADQVWTSNYQVMDDDWF
                     VHSRDISAWADNKNAVQIEFRIESSDPGTSFGWNIDEVIVKDRALPDYQACGGCAGAP
                     TFAGLETVSDPDACAGTGLVLAWQAAPGWGTGVSGSFEVHRGLTPDFAPGAANRVATG
                     LTGTSWTDANVPPDTPVWYVVRARNDESCTADGGLTDANTVRVGAVDSTAQSLPGPVG
                     ATLSIGTLGGVHTRLEWPPATGASTYSVLRATQADFSDAAEVGTTAGTLFEDPGAVTA
                     PTSYYYRVVAVDACGRSE"
     gene            5933..>6130
                     /locus_tag="VD788_17480"
     CDS             5933..>6130
                     /locus_tag="VD788_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78106.1"
                     /translation="MSYGIHQPSIASTSLRQARKERIAPDTSPVPPFSKGTNEFRESD
                     VLNEHRAFLGSVAAPQLDTVCG"
BASE COUNT          874 a         2175 c         2211 g          870 t
ORIGIN      
        1 cccgttcgtc ggctcctgcc gtgcggcgtg ctcgggggag ccgcgctggc cgttgccgcc
       61 ggcgccgccg gcgaaccgcc caccctgcgc ttcgacgacg cgccgcggtc ggggcaattc
      121 ggctcggtct cggtgtcggg cctgccggag ccggcgctgg cccgcctcgc cgagcggctc
      181 gagaactccg actgggagcg gctgctcgcc gtgcgcatcg gcgaaccggg ggccgcggcc
      241 gccccgccga ccgtgctcgg cagctacgag gtcacgggcg gcgtgctgcg cttccggccg
      301 cgcttcccca tcgcggccgg cctcgtgttc cacgcgcact tcgacggcgc gcgattcgac
      361 gactggagcg gcgcggcagc cgggactcgg acactgaccg cgacgttccg catgcccgag
      421 cctgccgagc gggcggcgcc gcgcgtggtc gtggtctacc ccagcgcgac ggagctgccg
      481 gagaacctgc tccgcgtcta cgtgcatttc tccgagccgg tctttcccaa ggacgtccac
      541 gatcaggtgc gtctgttcga cgccggcggc gagccggtcg agctgccgtt cgtcgagatc
      601 gagggcgggc tgtgggaccc ggcccggcgg cgcctgacgt tgttcctgca tcccggacgg
      661 atcaagcgcg gcgtcgcccc gaacacggag ctcgggccgc cgctgcgcgc cggggagtcg
      721 ttccggctgg ccgtcggcgg tggcttcgag aagccgatcc gcgcgctgcc cgccgaccgc
      781 gaggcgcccg atccggcgac ctggcgggtc ggcgcgcccg cggcgcgcgg cgagccgctg
      841 acgctcgact tccccgagcc gttggaccac gcgctgctcg ggcgcctgat cgaggtgcgc
      901 ggccccgatg gcgcgacact ccccggacgc gtgacgatct cgcacggcga gacgcgctgg
      961 accttcacgc ccgagcgacc gtggttcgcc ggcgaccacg tgctgcgaat cgacccggcc
     1021 ctcgaggacg tcgccggcaa cgcggtggcc ggcgcgttcg agcaccgcgg cacgacgcga
     1081 tcgccggcac cggccgagcc ggtcgagatc ccgttccgcg tgccgtgagt cgacgatccc
     1141 ggcgtcgcgc tacctctttc ctcgcgccgc ggtgaaccgt tcgccggcgc gggcccgtgc
     1201 gcgcgcggcc tccgtctcgc ggtccttcgg cgaggccgag gtgacgagcg aggcgagcag
     1261 ctcgcgcgcc accgccgtca ccgactcgac ggcacgctcg aatgcggcct ggttcgcgtt
     1321 cgatggtcgc gtgaaaccgc tcaatttgcg gacgaactgc aacgacgccg cccggatctc
     1381 gtcgtcggtc gcggggggtt cgaaattgaa cagcgtcctg atgtttcgac acatgacggc
     1441 accctcacct cgtgcgtagg atccgtgaga cgcctggccg ccgcgagtgc tctcattcta
     1501 gtacgaagat ttccagcggt tcgctttggg gggtgcggcc gcttttccgc ttgccggcgc
     1561 tctggttgtt gccgggacag atagagcggg tctggacccc ggggatcgtc gtcagtcgcc
     1621 cgatgctctc ggcaaaaggg cgggtgttct cctcgacacc ccggctagcg cgaggggcgt
     1681 cgcgcagggg acaacctcgg tcgcagaaag cgtcggcgcg tggggcgtag ggggattctc
     1741 cgctcgaggt aggggaacga cggatggcgc acgcgcgggg gatccggtac accccctccg
     1801 atcgggtcgt cgcctccgcg gtcgtggggc gcgaaggagc cccatgccgt acgctcggat
     1861 cgcctgcctc actctgctgc tcgcgatcgc cccgccggtc tcgatccacg ccggttcgga
     1921 tccggcgcgg atggatgccg ggatcctgcg cgtcgaagag cacgcgtcgc gggccgtcgc
     1981 cctcccgccg cgcgccaccg gtgccgggca acggctggcc cggatcgacg gcgcgctgta
     2041 ccagaagagc ggggagctct acctgccggt cgtgcccgac cggatcagcg tccggctggc
     2101 cgacgacgtc aagagctggg acgagctcgt cgcgcggacc gtcgcccgcg acccgaaggc
     2161 gtacgcgccg ctcgccgacc tggtggcgga gaggaccaac cggctgggcg tcgtcgacct
     2221 gcgggtgccg gaggggagcg acctgatccc ctggctcgat ctgctcgaga cgacgggcga
     2281 ggtgcgttac gcggaagtgg cgagccgcgg cgtctacctc gcgacccaga gcgacccgct
     2341 ctatccgcag cagtacgcgc tgaacaacac cgggcagacc gggggcaccc ccggcgccga
     2401 cgtcgacgcc gagcgcgcgt gggacctgac cgcgggcgac ccgtcggtga tcgtcgcggt
     2461 gctcgactcc gggacggcga tcgaccacga ggacctggcg gcgaacgtct ggcacaacga
     2521 cggggagatc ccgggaaacg ggatcgacga cgacgggaac ggattcgtcg acgactggga
     2581 aggctgggac ttcgccggta acgacaacga tccgcgttcg ggcaacgagc acggcacgtt
     2641 cgtcacgggg atcgtgaacg ccgcggggag caacggcgcc ggcgtggcgg gtctggccgg
     2701 gggtctcggc ggaccgggcg tgcgcgcgat gatcctgagc gtcggctcga gcgggccggt
     2761 gaccgacgtg atcgacgacg cgatcctcta cgcggccgat cgcggcgcac gcgtgatcac
     2821 gctgagcctc tcggtcggtg agacgcaggc gatcaacgac gcgctggact acgcctacaa
     2881 cctgcgcgac gtcttcgtcg actgcgccgc gggcaactcg ggctcggcgg tcagctaccc
     2941 cgcccttcga ccggaggtga tggccgtcgg ctccaccgat cacgacgacc tgagcagcgg
     3001 cttctccaat ccgggtcccg aggtcgaggt gtcggcgccc ggctcgagcg tcctgtcgac
     3061 ggccctgaac aacggttaca ccacgagcag cgggacgtcg ttcgccgcac cgcgggtcgc
     3121 ggccctggcc gccctgatct acagccgcaa cccgggtctg gccgcggccg acgtacgaca
     3181 gatcatcatc gacaccgccg aggacgtggg ggacccgggc ttcgacgagc gcacgggaca
     3241 cgggcggatc aacgcctacg aggccctgct gatcgcggcc gactccgacg gcgtgatccg
     3301 cctcgaccgc gcggcctacg cctgcgacgc gacggtcggg ctcaccgtct cggacgtcga
     3361 tctggcgggg agcgggacga tcgacgtgac cgcgtcgagc gagaccgaac cggccggcga
     3421 aacggtcacg ctgaccgaaa cgggcgccgg ttccggtctc ttccgcgggg cgctcgcggc
     3481 ggacggcgga gcggcgcagc cggacggccg gctgcagctc tcggacggcg acacgctggt
     3541 cgcggagtac gtcgaccagg acgacgggtc cggcggcacg ggggtgacga agacggccac
     3601 ctccacggcc gactgccgtg cgccgctcgt cgccgacgtc gccgtcgacg gcgtcacgac
     3661 ctcgacggcc acgatccact ggacgaccga cgaggcggcg gacacccggg tccgctacgg
     3721 ggacgacctg cccccgagcc agctcgagca gtccgccgga ttcgtctacc agcaccaggt
     3781 cgcgctgtcc ggtctcgatc agtgcacgat ctacaagttc gcggtcgagt cgagcgacga
     3841 cgtcggcaac ctggggatcg acgacaacga cgggacgtac ttcgtcttcg agacgctggt
     3901 ccaggtcccg ggcgtcggcg ccgtgccgtg tcatcaggcg ctgctggcct tcgacgacgc
     3961 gagcccggcc gggtgcgagg agggcgtcgg cctgtccctg ctcgacagcg atctcgacac
     4021 cgactccggt caggtggagt cggtccaggt ccgtctggcc tcgacccgcg agccggaggg
     4081 cgagtgggtc acgctgaccg agatcaaccc gcagagcagc cggttcgagg gtcaggggat
     4141 gctcgatcgg ggaccgcccg tcgcgggcga cggcctgctg gcggtggccg acggcgactt
     4201 cgtcacggcg acctatcacg accccgacga cgggcagggt cgtccctgga tcgccaccgc
     4261 gagcaagacc gccgactgcg aggcgccccg tatcggcgac gtgcgggtga cggagatcgc
     4321 cgcgacacgg gcggtgatcg agtggacgac cgacgagccc gggacctccc gcgtcgagtt
     4381 cggtccgacc gcggcgctgg ggaacagcgt cgaggacccg acgctgcgaa ccgaccaccg
     4441 cgtggtcatc tcggtcttcg acgcttgcga gcgcgtgcac ttccgcgtgt cgagcgtcga
     4501 cacccacggc gagacgcggg tcgccgacgc cgcgggggtg ccgttttcgt tcaacgtgaa
     4561 cgagatcccg ggtctggtgt tccacgagac gttcgagacc gacaccggct ggacgctccc
     4621 cgacgactgg gagcgcggca caccgcaagg tctgggtagc ggcgccggcg atccgaccgc
     4681 cgcctggagc ggtctgtccg tgatcggcaa cgacctctcg ggtctcggcg cgttcgccgg
     4741 agactacgag cccgtcacct cgagctcggc cctctcgccg gtgttctcga cacgccagca
     4801 acgcggtctc gagctgatcg tccaccgcaa gctcggcgtg acgagcacgg acgaggccgg
     4861 catttacgtc gtgaccagcg gcgccgatca ggtctggacc agcaactacc aagtgatgga
     4921 cgacgactgg ttcgtccaca gccgcgacat ctcggcatgg gccgacaaca agaacgcggt
     4981 acagatcgag ttccgcatcg agtcgagcga tcccggtacc tcgttcggct ggaacatcga
     5041 cgaggtgatc gtcaaggacc gcgcgttgcc cgactaccag gcgtgcggcg ggtgcgccgg
     5101 cgcaccgacg ttcgccggtc tcgagaccgt gtccgatccc gacgcttgcg ccgggacggg
     5161 gctggtgctg gcctggcagg cggcccccgg gtggggcacg ggggtgtcgg gcagcttcga
     5221 ggtccatcgc ggcctcacgc cggacttcgc ccccggcgcc gcgaatcgcg tggcgacggg
     5281 tctgaccggc acgtcgtgga ccgatgccaa cgtgcccccc gacacgccgg tgtggtacgt
     5341 ggtccgcgcg cgaaacgacg agtcgtgcac ggccgacggc ggtctgaccg acgcgaacac
     5401 cgtgcgcgtg ggggcggtcg acagcaccgc gcagtccctc cccggacccg tcggggcgac
     5461 gctctcgatc ggcacgctcg gcggcgtgca cacccgtctc gagtggccgc ccgcgaccgg
     5521 cgcatcgacc tactccgtcc tgcgggccac gcaagccgac ttctcggacg ccgcggaggt
     5581 cgggacgacg gcgggcacgc tgttcgagga tccgggggcg gtcaccgcgc cgacgtctta
     5641 ttactatcga gtcgtcgcgg tcgacgcctg cggtcgatcg gagtagcggt cgtccgcccg
     5701 acgtcgctgc gataccttcg cggcgaaggg gcacagccag gcgagcgcgt cggtcgcctc
     5761 ggcgccgcgg cctcccgccg cgagcacgcg accccagcgc gtggtgtcga acacgtccgt
     5821 catgcgcgaa ccggcgggac tccgtgtcgg ccgttcgagg ctatcctacc gcgtcgggtg
     5881 cccctggagc aggtcgagga tcgacccaca gagctcgagc cactctccgg cgatgtccta
     5941 cggtattcac caaccgtcga tcgcctcgac ctcgttgcgc caggcgcgca aggagcgtat
     6001 cgcgccggac acgtcgcccg tccccccgtt ctcgaagggc acgaacgagt ttcgcgagag
     6061 cgatgtcttg aacgagcacc gtgccttcct cggttccgtc gctgcgccac agctcgacac
     6121 cgtgtgcgga
//
LOCUS       DASPGN010000602         7395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_816655, whole genome shotgun sequence.
ACCESSION   DASPGN010000602 DASPGN010000000
VERSION     DASPGN010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816655"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VD788_17485"
     CDS             <1..664
                     /locus_tag="VD788_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78107.1"
                     /translation="DQADACLSPDRATFYYWESHGWDRPGRLLRVPVGGGPSDLVLER
                     SGAPWIACAGVADGPCLFFEYDDTLGIGVGQLDAEARAVDSVTTLDSETILGRPFALT
                     ATRTALSPDGRRIATIEDPVILLLDTATGEPVERIEITGIPAHDFSEIVWSPDGQGFY
                     VIGETGRRMILLHVDRKGAARVLEESQTARLFAVRPSPDGRHLALGKTILDANVWMVD
                     GR"
     gene            complement(1044..4322)
                     /locus_tag="VD788_17490"
     CDS             complement(1044..4322)
                     /locus_tag="VD788_17490"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HXV78108.1"
                     /translation="MDRGRGARVTVCHRPPGDAANARTIVVGQAAVPAHLAHGDFLGA
                     CPQGCGNGIVEDSEACDDGNTNPSDGCDNECHATEWNATTVVGTVIQATSFGLGDPTS
                     IALDGAGRVYIADMSNNRVERIDGTTLTRVAGTGVPGFGGDDGPAATAQLLTPRGVAV
                     DGLGRLYIADANNNCVRRVDTLGIITTIAGTCGLPHSYYTPGTNNTGDGGPARQATFA
                     FPQSVAVDGLGRVYIADAFNHVVRRIDETGIIDRVAGTGIAGGGGETGFATSADLYLP
                     GGLAVDGAGALYIADPGNQVIRKVDDPGPPSQPGTIHTVAGVFGQPGDSIDGPVATTR
                     LFNPAAAFVDHNGVLTIAENNRIRQVVGTDIVTVAGTNAWGFAGDDGPATSADLAYPQ
                     GVAVDATGRIFIADTDSNRIRRVDIDASRTITTIAGSGVYRVLGDDAAATSAPDLQPY
                     GVAADAQGNVYFADGFNSGDNTSHDMIRRVDTTGTITTIAGNGNRFVSPIPFPEAVAV
                     DSTGKVYVLTFGDGIVRRADATELVRVAGGGGSTAEGVLAIDANLNGARAIAFDSMDR
                     LYVSLSTTAGRIRRVDVPNGTTGTITTVAGGGSNTGDTTGPGAPLSARLNFPRGIAFD
                     AMGRLYVADAGSVRIVRIDFQVPSLTRIAGGTGNTQTAENVPPLLARMGPISVAVDAD
                     YNVYYADPVRHRVSKIDANLTGVTTFAGSQSRVPGDIGDGGPATGSVDVTRFNNLQGI
                     AFDADGRLLIADLGDRRVRRVDANGTITTIAGQVGPEGMGLAAHAQLADPRALLTLPG
                     MTLIAGGSSGTVQAVRAGFLEAVVGRYPQATPTTNLAKFRDGTFGMVAGIAADPTVSK
                     LYVVESHRIHGVTLTTPHDPDTWTIEAFANLAGTAGYADGSAATAQFRSPQGLFYDDT
                     ALYVADTGNHVIRQIAGTTVSTLAGSGEQGGFFGDGGPAWIALLDGPAAITKCSNGDL
                     FIADTGNNRVRRIHQDVISTVLGVGVADSSGNGAPASAFPVNRPKGLVCDAEGNLYIA
                     STDTIRQLAATNGVVDGTGPVYTIYESAKHICLTGITLIDSETLQVADQCVGDLVELR
                     RQRR"
     gene            5152..7110
                     /locus_tag="VD788_17495"
     CDS             5152..7110
                     /locus_tag="VD788_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HXV78109.1"
                     /translation="MTSLSPSFDAAPVRPLASRVPQELLRIPFAQDRPRTLRYEAGIV
                     LDTQLRRLSRASGAIDRALAMRLAVLHGSNGFVRLGYARFADWARERAGLPVRTAQEW
                     VRLGRALERLPRLAHALAAGHVSWSAACQVARVATSVDEAGWIALAASLPVRVLKARV
                     ARALAGERVAADDPNAAGEAAPSSPGGADGEAGGGGSTEVPSDEDPLDPLVLLELSVS
                     GEIARAWYAALELCELVAGAPLTPGEAPEYVLADFLSGGVPVDSSETGFRPSLPLKVP
                     RRGALRSVGRPEASPPGVDLDSRDATRFAPGAELRHALEVADAPVPRDPFGIDASVRA
                     LTAARTGLDLDLARLLRNFHMLSLASHLGYRDFSSYVEERLGISVARARFLERLDRRL
                     LCLERIRRAVREGVIGAVAALEIARVAVRGRTEQDWIDRAGRRSVARLREEVAWAERE
                     IGRCGNREIRPPELDRLPSDLEALGDWIASGCEMSARKPDPMGTSRVRFMLPESALSL
                     WTEARARLAVGHPRAFVSDAEVLWEVAVAFLVTHGPMWVEALENGDPIAVRDRFACQV
                     PGCSVRLGSAHHIRFRSQGGPDLSWNLVFLCTVHHLDGVHAGRIRVSGRAPDDLHFEL
                     GLRPDGSALERFAGHERIGELAASGLAL"
BASE COUNT         1075 a         2517 c         2546 g         1257 t
ORIGIN      
        1 cgaccaggcc gacgcgtgcc tgagtcccga ccgagcgacc ttctattact gggagtccca
       61 cgggtgggat cgcccgggaa ggctgcttcg tgtcccggtc ggcggagggc cgagcgatct
      121 cgtgctcgag cgctccggcg cgccgtggat cgcctgtgcc ggcgtcgcgg atgggccatg
      181 tctcttcttc gagtacgacg acacgctcgg gatcggtgtg ggtcaactcg acgcggaggc
      241 gcgagcggtc gactcggtga cgactctcga cagcgagacg attctcgggc gcccgttcgc
      301 cctgactgcc acgcgtaccg cactctcccc tgacggtcgg cggatcgcca cgatagagga
      361 cccggtgatc ctcctgctgg acaccgccac cggagagccc gtcgaacgca tcgagatcac
      421 gggcataccg gcgcacgact tcagcgagat cgtgtggtcg cccgacggcc aaggtttcta
      481 cgtcatcggg gagacggggc gcaggatgat tctgctgcac gtcgaccgga agggtgccgc
      541 ccgggtgttg gaggagagcc agaccgccag gctgttcgcg gttcgcccat cacccgacgg
      601 ccgccacctg gcattgggca agacgatcct cgacgcgaac gtctggatgg tcgacggccg
      661 ctgagacgac cctggcccgc gagcgtacgc caagccgctc gaggccctcc ggtcggaccc
      721 atccctcgag cccgtccacg cgcacgcgga tccacaggtc cgggcggtcc gcgtcgagcg
      781 actcgtcgac gagcggcgga tcgtcgagac acgcctcgag gaccacgggg ctagactggg
      841 ccggactgga catccgcgcc atgcgaggcg cacggcggcg cgcccacgac tacgacgcgg
      901 gtgcggtcat tcagcgccgc ggttccgtct gcgcctccgc acccttgtcg gctcgatgac
      961 gatcaggctg ccggcgagat catccgcatg tcgcgtcgtc tccccacgtc aattcctcgt
     1021 cgttggcgcc ggtcggtgag tgatcatcgg cgctggcgtc gtagctcgac gaggtcgccc
     1081 acgcactggt cggcgacctg gagcgtctcg ctgtcgatca gcgtgatgcc ggtgaggcag
     1141 atgtgcttcg ccgactcgta gatcgtgtag accgggcccg tgccgtcgac gacgccgttg
     1201 gtcgccgcga gctggcggat cgtgtccgtc gatgcgatgt agaggttgcc ttcggcgtcg
     1261 cacaccagac ccttcggccg gttgaccggg aacgcgctcg cgggcgcacc gttacccgag
     1321 gagtccgcga ccccgacccc gagcaccgtc gagatcacgt cctggtggat gcgccggacg
     1381 cgattgtttc cggtgtcggc gatgaacagg tcgccgttgc tgcacttcgt gatcgctgcc
     1441 ggtccgtcca gcagggcgat ccacgcgggc ccgccatcgc cgaagaatcc gccttgctcg
     1501 ccgctgcctg cgagggtcga gaccgtggtt cctgcgatct gacggatcac gtggttgccg
     1561 gtgtcggcca cgtacagggc ggtgtcgtcg tagaacaacc cctgcggcga tcggaactgt
     1621 gccgtcgcgg cgctgccgtc agcgtagccc gcggtgccgg ccaggttcgc gaacgcctcg
     1681 atggtccagg tgtcggggtc gtgcggcgtc gtcagcgtga cgccgtggat gcgatgggac
     1741 tcgaccacgt acagcttcga cacggtcggg tcggccgcaa tccccgctac cataccgaac
     1801 gtcccgtcgc ggaacttggc caggttcgtg gtcggcgtgg cttgggggta gcggccgacc
     1861 acggcttcga ggaaaccggc gcgcacggcc tgcacggttc ccgacgagcc acccgcaatg
     1921 agcgtcatac ccggcagcgt caacagcgcg cgcgggtccg cgagctgcgc atgcgcggcg
     1981 aggcccatgc cttcgggtcc cacctgcccg gcgatcgtgg tgatcgtgcc gttcgcatcg
     2041 acgcggcgca cgcggcggtc gccgaggtct gcgatcaaga gccgcccgtc ggcatcgaac
     2101 gcgataccct gcaggttgtt gaaccgcgtg acatccacgg agccggtcgc gggtccgccg
     2161 tcgccgatgt cgccgggcac gcgggactgc gatccggcga acgtcgtgac ccccgtgaga
     2221 ttcgcatcga tcttgctgac ccggtgtctg acggggtcgg cgtaatagac gttgtagtcg
     2281 gcgtccaccg cgacactgat cggacccatc cgcgccaaga gcggcgggac gttctcggct
     2341 gtctgcgtgt tccccgtccc ccccgcgatc cgcgtgaggc tcggcacctg gaagtcgata
     2401 cggacgattc gcaccgaacc ggcgtctgcg acgtagagcc ggcccatcgc gtcgaatgcg
     2461 atgcctcgcg ggaagttgag ccgagcggag agcggtgcac ccggcccggt ggtgtcgccg
     2521 gtgttcgatc cgcccccggc gaccgtggtg atcgttccgg tcgtaccgtt cgggacatcg
     2581 acgcgacgaa tcctacccgc cgtcgtgctc agcgacacgt acaagcggtc catcgaatcg
     2641 aacgcgatcg cgcgtgcgcc gttgagattc gcgtcgatcg cgagcacgcc ttccgcagtc
     2701 gaccctccgc ctccggcgac acgcaccagc tcggtcgcgt ccgcgcgccg cacgatcccg
     2761 tccccgaagg tcagcacata aaccttgccg gtcgagtcga ccgccacggc ctccgggaac
     2821 ggaatcggcg acacgaaccg gttcccgttg ccggcgatcg tggtgatcgt gccggtggta
     2881 tcgacgcgcc gaatcatgtc gtgcgaggta ttgtcgcccg aattgaagcc atcggcgaag
     2941 tagacgtttc cctgagcatc ggcggccacg ccatacggtt ggaggtcggg cgcgctcgtg
     3001 gcggccgcat cgtcaccgag aaccctgtat acgccgctgc cggcgatggt cgtgatcgtc
     3061 cgcgacgcgt cgatgtcgac gcgccggatc cggttgctgt cggtgtcggc gatgaagatg
     3121 cgccccgtcg catcgacggc gacgccctgg ggatacgcga gatccgctga ggtcgcgggc
     3181 ccgtcgtcgc ccgcgaagcc ccacgcgttg gttcccgcga ccgtcacgat gtcggttccg
     3241 acgacctgcc ggatccgatt gttttcggcg atcgtcagga caccgttgtg gtcgacgaac
     3301 gcagcagccg ggttgaacag ccgcgtggtc gcaaccggac cgtcgatgct gtcgccgggt
     3361 tgcccgaaca cgccggcgac cgtgtggatc gtcccaggtt gcgacggcgg ccccggatcg
     3421 tcgaccttcc ggatcacctg gttgccgggg tccgcgatgt acagcgcgcc cgcgccgtcg
     3481 acggcgaggc cgcccgggag gtagaggtcg gcactggtcg cgaatccggt ctcgcccccg
     3541 ccgcccgcta tcccggtccc ggcgacccgg tcgatgatcc cggtctcgtc gatccggcgc
     3601 acgacgtggt tgaacgcgtc ggcgatgtag acgcggccga gcccgtcgac cgcgacgctc
     3661 tgcggaaagg cgaacgtggc ctggcgtgcg ggcccgccgt ccccggtgtt gttcgtgccg
     3721 ggcgtgtagt acgagtgcgg aagtccgcag gtgcccgcga tcgtcgtgat gatgccgagc
     3781 gtgtcgaccc tgcgcacgca gttgttgttt gcgtcagcga tgtagaggcg gccgaggcca
     3841 tcgaccgcga cgcccctcgg cgtcaagagt tgcgcggtcg cggcggggcc gtcgtcgcca
     3901 ccgaagccgg gcaccccggt tccggcgacc ctcgtgagcg tggtgccgtc gatgcgctcg
     3961 actcggttgt tcgacatgtc ggcgatgtag acccggcccg cgccgtcgag ggcgatgctg
     4021 gtcggatcgc cgagcccgaa cgacgtcgcc tggatgaccg tgccgaccac cgtggtcgcg
     4081 ttccactcgg tggcgtggca ctcgttgtcg cagccgtcgc tcggattcgt gttgccgtcg
     4141 tcacaggctt cactgtcttc gacgatgccg tttccgcacc cctgggggca cgcgcccagg
     4201 aagtctccgt gcgcaagatg agccggcacc gccgcctgac ccaccacgat cgtccgcgcg
     4261 ttcgccgcgt cgcccggggg tcggtggcac acggtcaccc gcgcgccccg accacggtcc
     4321 atgatgtcgc tcaggctgga aggtcttgcg aggtcgagag ttctgcggcc gccagcgccc
     4381 caccccggag tcgccgagac gaccagaacc gagcacagcg cgatcaagaa caagaggcca
     4441 gctcgttcgt gtctcatgac tcgctccacc gcggaccacc ttcgatcggc ccggtcgcca
     4501 atatacggcg tccgaggcgc cgccaccgaa atgagacgaa taatccggaa actatttacg
     4561 cgctcgaaga cgagcaggag tagttcgcgg aggggacgga agcgttcttc ggtaccaacg
     4621 agttccaccc cttcgtgcgg cggagctatt gcgtcacgat ccgaacctcc aaaagctcga
     4681 gcgccttgcg gccgcctccc gtatcgtcgg ccaagagatc cttgttgacg tagacggtcc
     4741 aaccctcgac ggatctcctt tcgtacctgg ctccatcggc tccttcagct gagccagagt
     4801 ttcgtcggga cggctgatcg cgagtgtgcc gtcgagaatg acgaagcgat tcgggtcccc
     4861 atcggggtga tcgcagcacc ctccagccac gaggcatcag ccggcggtca gcgtcgcgat
     4921 cgcgcgtcac caaccgcggt ctccacgagt tggcgaagcg ggagtagatg accggcgagc
     4981 cccttaccac gacggcagtc gaggtcttgc agacggaccg ccgtgaaagg aggcacgcgc
     5041 ggcgacttca cttggttatg gattcgcaac accgttatcg acccgcggta tcgcaaccca
     5101 cgcgataacg cagcatactc ccgatgcacg ctttcattgg gagtccttcg cgtgacctcg
     5161 ctcagtcctt cgttcgatgc cgccccggtc cggccgctcg catcgcgcgt gccgcaggag
     5221 ttgctccgca ttcctttcgc gcaggaccgg ccacgcacct tgcgttacga agccggcatc
     5281 gttctggaca cgcagctgcg ccgcctgtcg cgggcgtccg gtgcgatcga tcgcgcgctc
     5341 gcgatgcggc ttgccgtgtt gcacggctcg aacggcttcg ttcgcctggg ctacgcgcga
     5401 ttcgccgact gggcgcgcga gcgtgccggc cttcccgtgc ggacggcgca ggaatgggtc
     5461 cgcctcggtc gcgcgctcga gcgcctcccc cggcttgccc acgctctggc cgcaggccac
     5521 gtgagctggt cggcggcatg ccaggtggcg cgtgtcgcga cgtccgtcga cgaagcgggg
     5581 tggatcgcgc tcgccgcatc gctgcccgta cgcgtgctca aggctcgagt ggcccgggcc
     5641 ctggccggcg agcgcgttgc tgcggatgac ccgaacgccg ctggcgaggc ggcgccatcg
     5701 tcgccgggcg gtgcggatgg cgaagccggc ggcgggggtt ccaccgaggt cccgagtgac
     5761 gaagacccac tcgatcctct ggttctgctc gagctgagcg tatccggaga gatcgcacgt
     5821 gcctggtacg ccgcgctcga gctctgcgag ctggtggccg gggccccgct cacgccgggt
     5881 gaagcaccgg agtacgtcct ggcggatttc ctctcgggag gcgttccggt cgactcctcg
     5941 gagaccggat ttcgaccgtc cctgccgttg aaggtccctc gccggggggc gttgcgttcc
     6001 gtcggtcggc cggaagcctc accgccaggc gtcgacctcg actcgcgcga cgccacacgg
     6061 ttcgcgcccg gcgccgagct tcgccacgcg ctcgaggtcg cggatgcgcc ggtgccgcgg
     6121 gatccgttcg gaatcgacgc ctcggtgcgt gcgttgacgg ccgcgcgcac cgggctcgat
     6181 ctcgatctgg cgcgcttgct gcgaaacttc cacatgctga gcctcgcgtc gcatctgggc
     6241 tatcgcgact tttcgagcta cgtggaggaa cgcctcggca tttcggtcgc tcgtgcgcgg
     6301 ttcctcgagc ggctcgaccg gcggctgctc tgtcttgaga ggatccgccg tgcggtccgc
     6361 gaaggggtga tcggcgcggt cgcggcgctc gagatcgctc gcgtcgccgt gcggggtcgg
     6421 acggaacagg actggatcga ccgggccggc cgccgcagcg tggcgcggtt gcgcgaagag
     6481 gtcgcctggg cggagcgcga gatcggtcgc tgcggcaacc gggagatccg cccgcccgag
     6541 ctggatcgat tgcccagcga tctcgaggct ctcggtgact ggatcgcatc ggggtgtgaa
     6601 atgtctgcgc gcaagccgga tccgatggga acgagccgcg tccgattcat gctgccggaa
     6661 tcggcgcttt cgttgtggac cgaagcccgc gcgcgcctcg cggtcggaca cccgagggcg
     6721 ttcgtctcgg atgcggaggt gttgtgggaa gtcgcggtcg cttttctcgt gacgcacggg
     6781 ccgatgtggg tcgaagcgct cgaaaacggc gatccgatcg cggtgcgaga ccgcttcgca
     6841 tgccaggtcc cgggttgctc ggtacgtctc ggttcggcgc atcacatccg cttccgctcg
     6901 caaggcggcc cggatctgag ctggaatctc gtcttcctct gcacggtgca tcacctcgac
     6961 ggggtccacg ccgggagaat ccgggtctcg ggacgcgctc cggacgatct gcacttcgag
     7021 ctcggactgc ggcccgatgg cagtgcgctc gaacgattcg caggacacga acggatcggg
     7081 gaactggccg cctccggcct cgccctctga acggcccgct tcccggcgat cggtaccgcg
     7141 cgaccgacgg caagctcaag cgcgaggtcg cgatcaaggt gctgccgcgg gggttcaccg
     7201 aggacccgga gcgcctcgcg cgcttcgagc gggaagcgca gcttgcacct cgtcgagcac
     7261 caggcgcgcg tcgccgatcg actgcaggcg ctcgcgcggc tctcgttccg cgactggcct
     7321 agccaccgtc cagcagcgtc gcgaaagcac ttggcaactc tgttcggatt tggtcgtgcg
     7381 ggatacgcgt agaat
//
LOCUS       DASPGN010000603         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_349478, whole genome shotgun sequence.
ACCESSION   DASPGN010000603 DASPGN010000000
VERSION     DASPGN010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349478"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VD788_17500"
     CDS             <1..389
                     /locus_tag="VD788_17500"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXV78110.1"
                     /translation="TMAAAGFVFVDRRNRTRAMQSLDDAARTIRNGRSVMLFAEGTRS
                     RDGTVQPFKKGPFHLALKAGVPVVPVAIRGSWAILKPRSLVVTPGVVDVRILEPLEVA
                     AWLPDDVQGLLGRVRQEIVSALGRAS"
     gene            386..1801
                     /locus_tag="VD788_17505"
     CDS             386..1801
                     /locus_tag="VD788_17505"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXV78111.1"
                     /translation="MSRPALDEARLVRRLSARLSADTVLGTLAEAARRTRAELCLVGG
                     YVRDAALGLPPGDLDLVGGRRVRALLGEIRERFDTAGFRFRRRGVTTWRFCLDGRPVD
                     LVDASRRGIEHDLFRRELTANAVAFDLVDRRLVDPARGLDDLGRRLLRGPRSDAFADD
                     PVRSLRLCRFVACMPGFSVSPRTTAEARRVALSLRRSPAERLRPELDKLLAGADPARG
                     LQMMRGLGLLAVVLPELSPLERCVAGRGRPDVWAHTLATLSVAAGPRRLPGLARVRQP
                     EQRAVLRWSLLLHDVAKPETLAFTTDGRPTFHGHEELGRRRGAALMRRLRVPETRRRR
                     VERLVLNHLRPGHLADGGASPRALARLARAMGDDLPLLVAHAACDARASGGPGQPARW
                     RRLAAVLTALLGAYDRRPAEPTTALVDGRDVMRVAGIAPGPRVGRLLERVRRRQRAGL
                     LRTRDQALAFLRRATRPGRRR"
     gene            complement(1823..>3001)
                     /locus_tag="VD788_17510"
     CDS             complement(1823..>3001)
                     /locus_tag="VD788_17510"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HXV78112.1"
                     /translation="VTVAAVVSQLGLQQAIVRFVAESIGREAPARARAAVVFAYRWVA
                     FGVVAVSGFILLGGGVWIVRDLWESPALAATMPIAAIWLAAISLQVLTSETFRGFKEL
                     ALASLFGGVIAGTVSLAVLSLWVVERGTATLEQAMWISAGATALSVAIGVTVLRRRLS
                     RLPSSGGTLSAADALPVALPMWGNSVTAYLLLQCPLWIIGAFRPEPEVGLYFGALRLV
                     TLVSMPLILVNLVVPPFIAELYARGETRRLERVLRTAATFAGLPAFAILLSFVFFGAP
                     ILGLALGTHYRAGAPILALLSIGYLANVWTGSCGITLTMTGFHHTVLRVTIGSTIFAV
                     VASLLVVRRHGTLGVAGVVCAAAVLHNLANWLAARYHTGMWTHVTIPTARDIRSLLSR
                     "
BASE COUNT          424 a         1100 c         1098 g          379 t
ORIGIN      
        1 ggacgatggc cgcggcgggc ttcgtcttcg tcgaccggcg caatcgcacc cgcgcgatgc
       61 agagtctcga cgacgcggcc cgtacgatcc gcaacggtcg ctccgtcatg ctgttcgccg
      121 agggcactcg cagccgcgac ggcacggtcc agccgttcaa gaaggggccg ttccacctcg
      181 cattgaaggc cggggtgccg gtcgttcccg tcgcgatccg cggatcgtgg gccatcctca
      241 agccgcgctc gctggtcgtc acgcccggcg tggtcgacgt gcggatcctc gagccgctgg
      301 aggtcgcggc ctggctgccc gacgacgtcc agggactgct cggtcgcgtt cgtcaagaga
      361 tcgtctcggc gctcgggcgc gcctcgtgag tcgtccggcg ctcgacgagg cccgactggt
      421 cagacgcctg tcggcgcgcc tctccgccga cacggtgctg ggaacactgg ccgaggcggc
      481 gcggcgcacg cgcgcggagc tgtgtctcgt cggcggctac gtccgcgacg cggcgctcgg
      541 cctgccgccg ggcgatctcg acctcgtcgg cgggcgccgc gtccgggcgc tgctcggcga
      601 gatccgcgag cgcttcgaca ccgcgggctt ccgcttccgg cgtcgcgggg tcaccacctg
      661 gcgcttctgc ctcgacgggc ggccggtcga tctggtcgac gcctcgcgcc gcggcatcga
      721 acacgacctg ttccgccgcg agctgaccgc aaacgcggtc gcgttcgacc tcgtcgaccg
      781 gcggttggtc gatccggccc gcgggctcga cgacctcggc cggcgactgc tgcgcggtcc
      841 gcggagcgac gcgttcgccg acgaccccgt ccgctcgctg cggctatgtc gcttcgtcgc
      901 ctgcatgccc gggttttccg tcagccctcg cacgacggcc gaggcgcgac gggtcgcctt
      961 gtcgctccgg cgctcacccg ccgagcggct gcggcccgag ctggacaagc tgctggccgg
     1021 ggccgatccg gcccgcgggc tgcagatgat gcggggcttg ggtctgctcg ccgtcgtgct
     1081 gcccgagctc tcgccgctcg agcgctgcgt cgccggacgg ggtcgacccg acgtctgggc
     1141 ccacacgctc gccacgctgt cggtcgccgc cggcccgcgc cggctgcccg ggctggcccg
     1201 cgtccggcag ccggagcagc gcgccgtcct gcgctggtcg ctgctgttgc acgacgtcgc
     1261 caaaccggag acgctcgcgt tcaccaccga cggccggccg acgttccacg gccacgaaga
     1321 actcggccgg cgacgcggcg cggcgctgat gcgtcgcctg cgggtgccgg agacccgcag
     1381 gcggcgggtc gaacggctgg tgctcaacca cctgcgcccc ggacacctgg ccgacggcgg
     1441 cgcctcgcca cgcgcgctcg cccggttggc gcgcgcgatg ggtgacgacc tgccgctgct
     1501 cgtcgcacac gcggcgtgcg acgcccgggc gtcgggcggc ccgggacagc cggcccgttg
     1561 gcgcaggttg gccgccgtgt tgacggcctt gctcggcgcg tacgaccggc ggccggccga
     1621 accgacgacg gcgctcgtcg acggtcgtga cgtgatgcgg gtggccggca tcgcgccagg
     1681 accgcgcgtc ggccggctgc tcgagcgggt gcggagaagg cagcgcgccg gactcctccg
     1741 gacgagggat caggccctcg ccttcctgcg acgggcgacg cgaccgggcc ggcgccggtg
     1801 acggcctcgt tcgaatcgtt cgctaacgcg aaagcagcga gcggatgtcg cgcgcggtgg
     1861 ggatcgtgac gtgcgtccac atgccggtgt ggtagcgcgc ggcgagccag ttggcgagat
     1921 tgtgcagcac ggccgcggcg cagaccacgc cggcgacccc cagcgtgccg tgacggcgaa
     1981 cgaccagcag cgaggcgacg acggcgaaaa tcgtcgagcc gatggtgact cgcagcaccg
     2041 tgtggtggaa gccggtcatc gtcagcgtga tgccgcacga accggtccag acgttcgcca
     2101 ggtaaccgat gctcagcagc gcgaggatcg gggcccccgc ccggtagtgc gtgccgagcg
     2161 cgaggccgag gatcggcgcg ccgaagaaga cgaacgacag caggatggcg aacgccggca
     2221 ggccggcgaa ggtggcggcg gttcggagca cgcgctcgag acgtcgcgtc tcgccgcgcg
     2281 cgtacagctc ggcgatgaac ggcggaacga ccagattgac caggatcagc ggcatcgaga
     2341 ccagcgtcac cagccgcagc gcgccgaagt agagaccgac ttcgggctcc ggccgaaacg
     2401 caccgatgat ccacagcggg cactgcagca gcagataggc ggtcaccgag ttgccccaca
     2461 tcggcaaggc gacgggtagc gcgtcggcgg ccgacagcgt cccgcccgac gacggcaagc
     2521 gggacagacg ccggcgcagt acggtcacgc cgatcgccac gctgagcgcg gtggcgccgg
     2581 ccgagatcca catcgcctgc tcgagcgtgg cggtcccgcg ctcgacgacc cagagcgaca
     2641 gcaccgcgag cgagaccgtc cccgcgatca cgccgccgaa caacgaagcc agcgcgagct
     2701 ccttgaaccc gcggaacgtc tccgaggtca gcacctgcag gctgatcgcc gcgagccaga
     2761 tcgccgcgat cggcatcgtc gcggccaatg cgggcgactc ccacagatcg cgcacgatcc
     2821 agaccccgcc gccgagcagg atgaaccccg agaccgcgac gacgccgaac gccacccagc
     2881 ggtaggcgaa cacgaccgcg gcgcgagcgc gggccggcgc ctctcggccg atcgactcgg
     2941 cgacgaaccg tacgatcgcc tgctgcaggc cgagctgcga gacgaccgcg gcgacggtga
     3001 c
//
LOCUS       DASPGN010000604         8404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1636823, whole genome shotgun sequence.
ACCESSION   DASPGN010000604 DASPGN010000000
VERSION     DASPGN010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8404
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1636823"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VD788_17515"
     CDS             complement(<1..778)
                     /locus_tag="VD788_17515"
                     /inference="COORDINATES: protein motif:HMM:NF017039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78113.1"
                     /translation="MAQTASLTPMFRQYRALKGDHPDAILLFRMGDFYEMFFEDAKIA
                     SELLELTLTARGKGTDNVAPMCGFPHHQLDGYTARLVRAGRRVAICDQVEDPKTAKGL
                     VRREVIRIVTPGTVTDPAQLESKDNAWVASVAVVGGRPAAAFLDASTGEFLAWEPAAD
                     EAAPWDALADRLRAFTPREIVHAEDLNWTGGRPGSRALWTPIDPYAFSGEVALAQLRR
                     QLGVASLDGFGLRDRLAASVAAGALLQYVQDTQRSGLEHVN"
     gene            850..1209
                     /locus_tag="VD788_17520"
     CDS             850..1209
                     /locus_tag="VD788_17520"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HXV78114.1"
                     /translation="MESQQLSQELSDQDRILLVFSYLGPLAVVSLVATRREFVKWHAK
                     QGLVLSLSVAAIYLVILRPLIHFSIHLWSGLAELFWAIAWLVAIGGLLMMLMCIIRGL
                     EGERFKLPFLGDLADRL"
     gene            complement(1217..2326)
                     /locus_tag="VD788_17525"
     CDS             complement(1217..2326)
                     /locus_tag="VD788_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78115.1"
                     /translation="MRSPAAVLSVVSICLVAAVVAAAEGAEPATAERAVELYDAGRYA
                     EAGEILERLEAEGKLDGGLIYRLYYCRRQANDPRATETLSRSIEPLEREAAGAKTLEP
                     SFYLVNAYQNLGRRAEMLEAARNATRRVETGALDEPATGLERFRLGKLYADSDNPEQA
                     ERWFRSAIDSFDLAADASNAAYDRWASGYLADAAFAREDYVAAAQYYGRLVERGEPTT
                     TGLDRLATARARAGLYQEAGKAWRQAELLDPANANRARYCYRLAEAAARLPGVSAAAP
                     DGRLWTQLSADELTRTMQEQAAAVKAVHERIEAAKPLDQEERSRFRTELEQARPVFVA
                     AALEFALQGHNIRETAFFGGYAPLIFHDRSWQVPP"
     gene            2367..3065
                     /locus_tag="VD788_17530"
     CDS             2367..3065
                     /locus_tag="VD788_17530"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HXV78116.1"
                     /translation="MASKRATTNGTKRRLAALCVATGLAAAATVAADDRARPRDAGTA
                     VAGLQAWLDATDDLEAAFSQALVSGAFGAGLEEAGRLYVKRPGRMRWDYRDPDRKIAI
                     VDGDRTWLYLEEEAQLMLGRLGEHGTLLPALIVGDRALDELFEAELVPAAEGQRDGTV
                     GLRLRPRPAADSFESVLVTLRPPEFAIERAEVLDAAGNRMLYRFSKLKRNRGLPDGLF
                     RLAPPDGTSVVGSH"
     gene            3206..4237
                     /locus_tag="VD788_17535"
     CDS             3206..4237
                     /locus_tag="VD788_17535"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXV78117.1"
                     /translation="MPRVDDAARVLSGPASTKSEIGRLVGPELIEVERVFRDSLASPL
                     SIVDEIGSFIAEGGGKRIRPTLHLLSTKLCGYDGPHAVLMATVLEFIHSATLIHDDII
                     DQATTRRGLQSVNSRWGNDVTVLFGDYLFAKAMQLALRAESIPIMDRLAEVTLSMTEG
                     EMLQTRYAGRLDLSVDEYLDLVQRKTAGLFSCCCDLAAMLAGVGPDRQSALHGFGSEL
                     GMAFQIVDDLLDLTGDSRTMGKPAASDLREGKATLAVIELLRSGGREAAEGRELAERL
                     MGDGPALEPSAVARLTELLAETGAIDRARGRAERHARSAASCLDIFDDSPARRALGVL
                     PELVLTRDR"
     gene            4435..8025
                     /gene="smc"
                     /locus_tag="VD788_17540"
     CDS             4435..8025
                     /gene="smc"
                     /locus_tag="VD788_17540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HXV78118.1"
                     /translation="MLRLEKMEISGFKSFSDRTEVRFPPGITAVVGPNGCGKSNIGDA
                     INWALGEQSAKTLRGKQMADVIFAGSEARKPVGLAEVSLHLRGTEGLDHSDSGAIVIT
                     RRLFRSGESEYLLNGKRARLKDIHDLLDQGHVGARTYATIEQGRIDQILNAKPKERRL
                     LIEDAAGIAGYKHKRRLTELKLEATQANLLRVNDIVVEVERQIRALKRQAARARRYRA
                     LRDELRGTERIQFAVRSLELDAVLDQARRAASAAGAAEAEAAARLSALEVRLLDERGG
                     LETTNRSLRESADGLHRADLEIDRAESRIRASRERIAEAEQTIVRQQQEVETLERAVT
                     SRAEELDRRQRAADSVAVEVEGTRAELATEQCALDGIEAELRGVREELERLRRAQFES
                     MARAAESRNRLAGTEEGLQRIEAQLARLEQDRAAARDDLTRLSDETATLGQRCRDQRQ
                     RVDGSRGELGRCERALVEARGDLASRSEALASAREDEQSGRARLATLEDVSTRFAGVS
                     DGVRALIAASSAGGVKTRGVVADFIEAATDVEGVAEGYLEAFLPTVILDSDDDLCDAA
                     RLLRSSGAGRTSLICRSQPAGGLAVGTSPNGHGDDTAGVLDDPRVIGRLRERLTLRAT
                     ANGFIRDRLGDAIVVESLEAALDLHRRFPAADYLTRDGDVVYASGVVHAGGREVSDDG
                     LLAHQRRTREARERLDAAGAVARARLGEVEAARRAVDAAEQALVACRTELEASERRLV
                     ELELVSARAAEDRQQVDRRSGVLAGEVDALRSEREQLRTALVELGRIAADCDADCRRI
                     EGALRERTEVADRREEEVRMRAERTAVLRETLAGRLQRLESAEQERSALAEAVADWNG
                     RLARARGEIDAAHAREAEARELLACTEDELTSQLGVRERLRGAVAETERRIAELERTL
                     AGLDRETTAARQAVDRLRETTRETELSRTRAESSREHLDELCAQEIGCTAETAVRECT
                     AAGVDLAAVAFDTVEAEVARIKQALEAIGPVNMTAIEEFAELEQRHGFLTTQRLDLER
                     SMESLKETIRRVNRSSRERFVEAFEAIRGHYQQIYRSLFNGGRADLKLEEGEDVLECG
                     IEILAQPPGKRLSSVNLLSGGEKALSAIALLFAIFRFQPSPFCLLDEVDAALDEANVG
                     RFARMLVDYTENTQFIIVTHNKLTMESADLLYGVTMEEPGVSRLISMKLD"
     gene            complement(8027..>8404)
                     /locus_tag="VD788_17545"
     CDS             complement(8027..>8404)
                     /locus_tag="VD788_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78119.1"
                     /translation="YVACLSATHVPDHWRSKSVLGRDFTSMPRHRLLGLIRGAATEEA
                     AGGGQANVLRLDLPAELVFELDPDQPHVLSVGLAVEVDGRFHRVDSVETEERLATGET
                     PLTVRLTPGPDLDPASIDLAFAD"
BASE COUNT         1235 a         2865 c         3087 g         1217 t
ORIGIN      
        1 tgttgacgtg ctcgagaccc gagcgctgcg tgtcctggac gtactgcagc agcgcaccgg
       61 cggcgaccga agcggcgagc cgatcgcgca gcccgaagcc gtcgagcgac gcgacgccga
      121 gctgccgtcg cagctgcgcc agcgcgacct cgccggaaaa ggcgtacgga tcgatcggtg
      181 tccacaacgc gcgcgatccc ggccggccgc ctgtccagtt caggtcctcc gcgtggacga
      241 tctcgcgcgg ggtgaaggcg cgcaggcgat cggccagcgc gtcccacggc gcggcctcgt
      301 cggccgccgg ctcccaggcg aggaactcgc cggtcgaggc gtcgaggaag gccgccgccg
      361 gtcggccgcc gacgacggcg acgctcgcga cccaggcgtt gtccttcgac tcgagctggg
      421 ccgggtcggt gacggtgccc ggcgtgacga tgcggatcac ctcgcggcgg acgagcccct
      481 tggccgtctt cgggtcttcg acctggtcgc agatcgccac gcgccggccc gcgcggacca
      541 gacgcgcggt gtagccgtcg agctggtgat gcggaaagcc gcacatcggc gcgacgttgt
      601 cggtgccctt gccgcgtgcg gtcaacgtca gctcgagcaa ctcgctcgcg atcttcgcgt
      661 cctcgaagaa catctcgtag aagtcgccca tgcggaacag caggatcgcg tccgggtggt
      721 cgcccttgag tgcccgatac tgccggaaca tgggagtcag ggaggcggtc tgcgccatgg
      781 ccgggcggag tatagcaccg ggtgcgcgcg gcttgacgct gtcggcgcac ggcgatataa
      841 aggccgcgga tggaatccca gcaactctcc caggagctga gcgaccagga ccggattcta
      901 ctggtcttct cctacctcgg tccgttggcc gtcgtctcgc tcgtcgccac tcgccgcgag
      961 ttcgtcaagt ggcacgccaa gcaggggctg gtgctgtcgc tttccgtcgc cgcgatctac
     1021 ctggtgatcc tgcggccgct gatccacttc tcgattcacc tgtggagcgg actggccgag
     1081 ttgttctggg ccatcgcctg gcttgtcgcg atcggcggct tgttgatgat gctgatgtgc
     1141 atcatccgcg gcctcgaggg cgagcggttc aagctgccgt ttctcggcga cctcgccgac
     1201 cgtctgtgaa tcgcggctac ggcgggacct gccaggatcg gtcgtggaag atcaacggcg
     1261 cgtagccacc gaaaaaggcg gtctcgcgga tgttgtgccc ttgcagcgcg aactcgagcg
     1321 ccgcggcgac gaagaccgga cgcgcctgct cgagttccgt gcggaaccgc gaccgttctt
     1381 cctggtcgag cggcttggcc gcctcgatcc gttcatgaac cgccttgacc gccgcggcct
     1441 gctcctgcat cgtccgcgtc agctcgtcgg cggagagctg ggtccacagc cgcccgtcgg
     1501 gcgccgccgc cgagaccccc gggaggcgcg ccgccgcttc ggccagccgg tagcagtagc
     1561 gcgcgcggtt ggcgttggcc gggtcgagca gctcggcttg ccgccaggcc ttgcccgctt
     1621 cctggtacag tccggcacgg gcgcgcgccg tcgccagtcg atcgagaccc gtcgtcgtcg
     1681 gctcgcctcg ctcgaccagc cgaccgtagt actgcgccgc ggcgacgtag tcttcccggg
     1741 cgaacgcggc gtcggcgaga tagcccgacg cccaccggtc gtaagctgcg ttcgacgcgt
     1801 ccgccgcgag atcgaacgag tcgatcgccg acctgaacca gcgctccgcc tgctccgggt
     1861 tgtcggaatc ggcgtacagc ttgcccagcc ggaaccgttc gaggccggtc gccggctcgt
     1921 ccagcgcacc tgtttcgacg cgacgggtgg cgttgcgcgc ggcctcgagc atctcggcgc
     1981 gacggcccag gttctggtag gcgttgacca ggtagaacga cggctcgagc gtcttggcgc
     2041 cggccgcctc ccgctcgagc ggttcgatcg accgcgacag cgtctcggtg gcgcgcgggt
     2101 cgttcgcctg acgccggcag tagtagaggc ggtagatcag gccgccgtcg agcttccctt
     2161 cggcctccaa ccgctcgagg atctcgccgg cctcggcgta gcggccggca tcgtacagct
     2221 cgaccgcccg ttccgccgtc gcgggctccg cgccctcggc cgcggcgacg accgcggcga
     2281 cgagacagat cgaaacgacc gacaggaccg cggcgggtga tctcatcgaa gcctcatggc
     2341 acactggtta ccaccggagg acggctttgg caagcaaacg ggcgacgacc aacggaacga
     2401 agcggcgcct ggccgcgctc tgcgtcgcga ccgggctcgc cgcggccgcg accgtcgccg
     2461 cggacgaccg cgcccggccg cgggatgccg gcacggcggt cgccgggttg caggcgtggc
     2521 tggatgccac ggacgacctc gaggcggcgt tcagtcaagc gctcgtctcg ggtgcgttcg
     2581 gcgcggggct cgaggaagcg gggcgactgt acgtcaagcg gccgggacgc atgcgctggg
     2641 actaccgcga cccggaccgc aagatcgcga tcgtcgacgg cgaccgcacc tggctctacc
     2701 tcgaggaaga ggcgcagttg atgctcgggc gtctgggcga gcacggcacg ctgctaccgg
     2761 cgctgatcgt cggcgaccgt gcgctcgacg agctgttcga agccgaactc gtgccggcgg
     2821 ccgaagggca gcgcgacggc acggtcggtc tccgcctgcg gccgcggccc gccgccgatt
     2881 cgttcgagtc ggtgctcgtg acgctgcggc cacccgagtt cgcgatcgag cgcgcggagg
     2941 tgctcgatgc cgcggggaac cgcatgctct accgcttctc gaagctcaag cgcaaccgtg
     3001 gattgcccga cggtctgttc cgcctcgcac cacccgacgg aacctcggtc gtcggctcgc
     3061 actgagcggc ggcggggccg caacatccat ttgccgcggg ggggtgcgga tgttagtatc
     3121 cacccctgtt tttcctgttg ctgcagaagg gtttaagcgc gaagcgcccg atcgggcggt
     3181 ggccggagcg acgattttga ccaacgtgcc gagggtcgac gacgccgcgc gcgtcctctc
     3241 cggtccggcg tcgaccaaga gcgagatcgg tcgtcttgtc ggtccggagc tgatcgaggt
     3301 cgagcgagtg ttccgcgaca gcctggcgtc gccgttgagc atcgtcgacg agatcggctc
     3361 gttcatcgcc gagggcggcg gcaagcgcat ccggccgacg ctgcacctgc tcagcaccaa
     3421 gctctgcggc tacgacggcc cgcacgcggt gctgatggcg accgtgctcg agttcatcca
     3481 ctcggcgacg ctgatccacg acgacatcat cgaccaggcg acgacccgcc gcggtctcca
     3541 gtcggtcaac tcgcgctggg gcaacgacgt caccgtgctg ttcggcgact atctgttcgc
     3601 caaggcgatg cagctcgcac tgcgtgccga gagcattccg atcatggatc ggctggccga
     3661 ggtcacgctg tcgatgaccg agggcgagat gctgcagacg cgctacgcgg gccgtctcga
     3721 cctgagcgtc gacgagtacc tcgacctcgt gcagcgcaag accgccgggt tgttctcgtg
     3781 ctgctgtgac ttggcggcga tgctggccgg cgtcgggccg gaccggcagt cggcgctgca
     3841 cgggttcggc tccgagctgg gcatggcgtt ccagatcgtc gacgatctgt tggacctgac
     3901 cggggacagc cggacgatgg gcaagccggc ggcgagcgac ctgcgcgagg gcaaggcgac
     3961 gctggcggtg atcgagctgc tgcgctcggg cggccgtgag gcggccgagg gccgggagct
     4021 ggccgagcgg ctgatgggcg acggcccggc gctcgaaccg agtgcggtgg cgcggctcac
     4081 ggagctgctg gccgagaccg gggcgatcga ccgcgcgcgc gggcgggccg agcggcacgc
     4141 ccgatcggcg gcctcgtgcc tcgacatctt cgacgattcg cccgcacggc gagcgctcgg
     4201 ggtgctgccg gagctggtgc tgacccgcga ccgctgagta caatgtcccg cgatgaaggt
     4261 ggtggtcccg ccggccggtc gaagtcgatc ccccggaccc ccaatatctt gggttcggcc
     4321 gtcttcgtgc tccagtatgt aggggcgcag ttgttcgctc gagggtacct ttggtatagt
     4381 ctcgccggct ccgccccgtg acgtcgccgt ctccggccga ggaatcccgc gccgatgctc
     4441 agactcgaaa agatggaaat ctcgggcttc aagtcgttca gcgaccggac cgaggtccgc
     4501 ttcccgcccg gcatcaccgc cgtcgtcggt ccgaacggct gcggcaagtc gaacatcggc
     4561 gacgcgatca actgggcgct gggtgagcag agcgcgaaga cgctgcgcgg gaagcagatg
     4621 gccgacgtga tcttcgccgg cagcgaggcg cggaagccgg tcggcctggc cgaggtgtca
     4681 ttgcacctgc gcggcaccga gggtctcgac catagcgact cgggtgcgat cgtcatcacc
     4741 cgccggctgt tccgcagcgg cgagagcgag tacctgctca acggtaagcg ggctcggctc
     4801 aaggacatcc acgacctgct ggatcagggt cacgtcggcg cgcgtaccta cgccacgatc
     4861 gagcagggcc ggatcgacca gatcctgaac gccaagccga aggagcgccg gttgctgatc
     4921 gaggacgcgg ccgggatcgc cggctacaag cacaagcggc gcctgaccga gctcaagctc
     4981 gaggccaccc aggccaacct gctgcgcgtc aacgacatcg tcgtcgaggt cgagcgccag
     5041 atccgtgcgc tcaagcggca ggccgcccgc gcccggcgct accgcgcgtt gcgcgacgag
     5101 ctgcgcggca cggaacggat ccagttcgcg gttcggtcgc tcgagctcga cgcggtgctc
     5161 gaccaggcgc gccgagcggc gagcgcggcc ggggcggccg aggcggaagc cgcggcccgc
     5221 ctgtccgcgc tcgaggtccg actgctcgac gagcgcggcg gtctcgagac gaccaatcgc
     5281 tcgttgcgcg aatcggccga cggcctgcac cgggcggacc tcgagatcga ccgcgcggag
     5341 tcgcgcatcc gggcctcgcg cgaacggatc gccgaggccg agcagacgat cgtgcgccag
     5401 cagcaagagg tcgagacgct cgagcgcgcc gtcacctcgc gtgccgagga gctcgaccgg
     5461 cgccagcgcg cggccgattc cgtcgccgtc gaggtcgagg gcacgcgggc cgagctggcc
     5521 accgagcagt gcgcgctcga cgggatagag gccgagctgc gcggcgtgcg cgaggagctc
     5581 gagcggctgc ggcgcgcgca gttcgaatcg atggcgcgcg cggcggagag ccgcaatcgc
     5641 ctggccggta cggaggaggg gctgcaacgg atcgaggcgc agctcgcgcg tctcgagcaa
     5701 gatcgcgccg cggcgcgcga cgacctgacc cggttgtccg acgagacggc gacgctcggc
     5761 cagcggtgcc gcgaccagcg gcaacgcgtc gacggctcgc gcggcgagct gggtcgttgc
     5821 gagcgggcgc tggtcgaggc ccgcggcgac ctggcgtcgc gcagcgaggc gttggcgtcg
     5881 gcgcgcgagg acgagcagtc cgggcgggcg cgcctcgcga cgctcgagga cgtcagcacg
     5941 cgcttcgccg gggtgtcgga cggcgtgcgg gcgctgatcg ccgcatcgtc cgccggcggc
     6001 gtcaagacgc ggggcgtggt cgccgatttc atcgaggccg cgaccgatgt cgagggtgtg
     6061 gcggagggct acctcgaggc gttcctgccg accgtgatcc tcgacagcga cgacgacctc
     6121 tgcgacgcgg cgcggctgct gcgatcgtcg ggcgcgggtc gaaccagtct gatctgccgc
     6181 tcccagcccg cgggaggtct cgccgtcggc acctccccca acgggcacgg cgacgacacg
     6241 gccggggtcc tcgacgaccc gcgggtgatc ggacggcttc gcgaacggct gacgttgcgc
     6301 gcgacggcca acggcttcat ccgcgaccgt ctgggggacg cgatcgtggt cgagagcctc
     6361 gaggcggcgc tcgacctcca ccggcggttc cccgccgccg actacctgac gagggacggc
     6421 gacgtggtct acgcctccgg cgtcgtgcac gccggaggcc gcgaagtctc ggacgacggc
     6481 ctgctcgcgc accaacgacg aacccgcgag gcgcgcgagc ggctcgatgc ggcgggcgcc
     6541 gtggcccgcg cgcggctcgg cgaggtcgag gcggcccgac gagcggtcga cgcggccgag
     6601 caggcgctcg tcgcctgccg caccgagctc gaagcgtccg agcgccggct ggtcgagctc
     6661 gaactggtct cggcgcgagc ggcggaagat cggcagcagg tcgaccgccg tagcggcgtc
     6721 ctggcgggtg aggtcgacgc gctgcgcagc gagcgcgagc agctgcggac cgcgctcgtc
     6781 gagctgggtc ggatcgcggc ggactgcgac gccgactgcc ggcggatcga gggtgcgcta
     6841 cgcgagcgca cggaggtcgc ggaccggcgg gaggaagagg tcaggatgcg ggccgagcgc
     6901 acggcggtgc tgcgcgagac gctcgccggg cgcctgcaac ggctcgagtc ggccgagcaa
     6961 gagcgctcgg ccctcgccga ggcggtcgcc gactggaacg gtcgtctcgc ccgcgcgcga
     7021 ggcgagatcg atgccgcgca cgcgcgggag gcggaggccc gcgagctgtt ggcctgcacg
     7081 gaggacgagc tgacctcgca gcttggcgtg cgcgagcggc tgcgcggtgc cgtcgccgag
     7141 accgagcggc ggatcgcgga gctcgagcgg acgctcgccg gtctcgaccg cgagacgacc
     7201 gccgcacggc aggccgtcga tcgcctgcgc gagacgacgc gcgagaccga gctctcccgc
     7261 acgcgcgccg agtcttcgcg tgagcacctc gacgagctgt gcgcgcagga gatcggctgc
     7321 acggccgaga cggcagtgcg cgagtgcacc gcggccggag tcgatctggc ggccgtcgcc
     7381 ttcgacacgg tcgaggccga ggtcgcccgc atcaagcagg cgctcgaggc gatcgggccg
     7441 gtcaacatga cggcgatcga ggagttcgcc gagctggagc agcgtcacgg gttcctgacg
     7501 acccagcggc tcgatctcga gcgctcgatg gagtcgttga aggagacgat ccggcgggtc
     7561 aatcgttcgt ctcgcgaacg gttcgtcgag gcgttcgagg cgattcgcgg gcactaccag
     7621 cagatctacc gctcgttgtt caatggcggc cgggcggatc taaagctcga ggaaggcgag
     7681 gacgtgctcg agtgcgggat cgagatcctg gcccaaccgc cgggcaagcg cctgtcgagc
     7741 gtcaacctgc tgtcgggcgg cgaaaaggcg ctgtccgcga tcgcgctact gttcgccatc
     7801 ttccgcttcc agccgtcgcc gttctgcctg ctggacgagg tcgacgcggc gctcgacgag
     7861 gcgaacgtcg gccgcttcgc gcggatgctc gtcgactaca ccgagaacac gcagttcatc
     7921 atcgtcaccc acaacaagct gacgatggaa tcggcggatc tgttgtacgg cgtgacgatg
     7981 gaagagcccg gcgtctcccg gctgatctcg atgaagctcg actgactcag tcggcgaacg
     8041 ccaggtcgat cgaggccgga tcgaggtcgg gccccggcgt cagccgcacc gtcagcgggg
     8101 tctcaccggt cgccagtcgc tcctcggtct cgaccgagtc gacgcggtgg aaccgaccgt
     8161 cgacttcgac ggccagtccg accgacagca cgtgcggctg gtccggatcg agctcgaaga
     8221 cgagctccgc gggcaggtcg aggcgcagca cgttggcctg gccgccgccc gcggcctcct
     8281 cggtcgccgc cccgcggatc aaccccagca agcggtgacg cggcatgctg gtgaaatcgc
     8341 gtccgagcac cgacttgctg cgccagtggt ccggcacgtg ggtcgcggac aggcaggcga
     8401 cgta
//
LOCUS       DASPGN010000605        16934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_349538, whole genome shotgun sequence.
ACCESSION   DASPGN010000605 DASPGN010000000
VERSION     DASPGN010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 16934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16934
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349538"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VD788_17550"
     CDS             <1..592
                     /locus_tag="VD788_17550"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HXV78120.1"
                     /translation="AIFDRGSIWHTAVLPVGGDHFTNDLAVGLRTPIPDAERIKKKHG
                     CALSSLIQQNEAVEVPTVGGRRPRLLSQQVMAEILQPRAEEIFTLLRDEVSRAGFDNL
                     LNAGVVLAGGCVLMPGMTEVAEQVFEMPVRQGEPGGAGGLVDPACGPQHTAAIGLAVY
                     CAKHRKEARRAPLGVPIGPLGRVGDRVRTWFSGIFF"
     gene            617..1849
                     /gene="ftsZ"
                     /locus_tag="VD788_17555"
     CDS             617..1849
                     /gene="ftsZ"
                     /locus_tag="VD788_17555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXV78121.1"
                     /translation="MITMDDRNARARIGLTFDESRASQANIKVVGVGGGGGNAVSRMI
                     AARVEGIQFIAANTDCQALRNNGAPIKLQLGSKLTKGLGAGGNPEVGKSAALEDTEKV
                     LEALHGADMVFVTTGLGGGTGTGAAPIIANLAREMGALVVAVVTLPFAFEGRRRRTQA
                     EQGLHTLREVVDTVITIPNDKLLHTVERGTPISEAFNVCDDVLRQAVQGISDLITVPG
                     EINLDFADVKTVMSGMGMALMGTGVAEGEHRAVEAAQRAISSPLLEDASIHGARGVLI
                     NITGGEDMSLHEVSEAAAIVQEAADPDANIIFGTVIDPYHEHKVKVTVIATGFMREQS
                     RGLPLIEPVLESSSARRESTVPPIATPPEPQQTDRFFRPAVGETLDFDANDDGFTPNF
                     GKMKDDLDVPAFLRKQMD"
     gene            1849..2781
                     /locus_tag="VD788_17560"
     CDS             1849..2781
                     /locus_tag="VD788_17560"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXV78122.1"
                     /translation="MTQRILVIDDDPGTSTVVRSWFKGEPYEILAAENGERGLTMARA
                     ERPDVILLDLKMPGMDGISVARELKNDPLTRNTPVLVLTACRDVDAKVEAFNAGTDDY
                     VTKPFEFEEVDARIRSMLKRRDALVGLEATVEELNDTNAQLEQLLMIDEKTGLYNFRE
                     FRHRLRAEWSRAARYETPLSLVFLDIDDFKKLNDTLGHAAGDRILAEFATLVTGGARA
                     NDVAARYGGEEFSIVLPHTDGEMALRVAERIRRAVAEFTFIADERPTRITVSAGVATY
                     PSTAGLSTVDDLVRAADLAMYRAKDRGKNCVVRA"
     gene            complement(2787..4322)
                     /gene="lnt"
                     /locus_tag="VD788_17565"
     CDS             complement(2787..4322)
                     /gene="lnt"
                     /locus_tag="VD788_17565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXV78123.1"
                     /translation="MHRWATHRFTLPLIAGVALGVGYYTELLLPLLLALTPVLYWLDR
                     NGERSRWYRFTGSFVFGFAAAIIGLHFCYAMLEFSWLAAVLWVWFAGLLALKLSSCLM
                     VASWLRRRTGFSWGWTLPIVWVPLDWLLTFGDLRMTGDHAAHAMAAYPFLIQFADLIG
                     HYGVSALVYVVNGLAYDAMLAERRRSRRGAAIALGCLSAAVLAYDAWCWFGPLPERET
                     VRVVYIQPDIPLLVKRGEGTEAEQRSRLAALSRDAAALDPQLVIWPESARPDPLEHWL
                     DAPATYAMPEVQQLARELDSEFLVGVEYYAIRDRDDYDAYNAAVLVRADGRLDPGWVA
                     KVYLVPFVEATPFRAIFGPWVEERDGEWRWLAGGFLPGPRARTLETAAGPAGVLVCYE
                     LLFPDLPRELRNAGARFQVVITNDAWFGRTPFQRFLSNATRLRAIENRSEFVRAANSG
                     ISGFIDARGRFHERTGLFEVAIAARDVRLSDQRTVYDRIGDSVVGLLALGLVSTAVVA
                     RRR"
     gene            complement(4337..5635)
                     /gene="egtB"
                     /locus_tag="VD788_17570"
     CDS             complement(4337..5635)
                     /gene="egtB"
                     /locus_tag="VD788_17570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HXV78124.1"
                     /translation="MSTTATDRSTPRPRRGRDELAGRYDEVRHTSLALIDGLSAEDCV
                     VQSMPLASPAKWHLAHTTWFFETFVLEAGMPGFRPFEADFRVLFNSYYNTIGEQYSRA
                     DRGLLTRPSLDRVLAYRRHVDDQIRRCLAEPGLARDTVDVAGLVEVGIHHEQQHQELL
                     VTDVKHLLSRNPTHPVLRRCEESPVARPGPIEWVAFDEGVRHIGYDGEGFCFDNETPR
                     HRVWLEVFRLASRPVTNAEYLEFIDDGGYLEPGLWLADGWAVAQREGWRHPAYWLGSA
                     GRRMTFTASGLKELRLDEPVCHVSYYEAEAYARWRGHRLPTEAEWEVAASGLAVDGNF
                     LDDGRLHPVPAAAGGARLEQMMGNVWEWTASPYVGYPGYRPLAGALGEYNGKFMCNQL
                     VLRGGSCASPRSHLRTSYRNFFYPDARWQFSGIRLARDGA"
     gene            complement(5632..6735)
                     /locus_tag="VD788_17575"
     CDS             complement(5632..6735)
                     /locus_tag="VD788_17575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXV78125.1"
                     /translation="MTSISELDELTRVREPQDARERELEHARDLRDARRQLIEVLGEL
                     PSTTRTIVLDGVPPERDGHWPIDYLEACYLRLARLGAARHDDAITPLFERARELRRRL
                     HRAREALVVGNLALVPAIVRRYDRGVIPFADLVQEGHVGLIKAVDRFDPDRGYRFSTY
                     ASWWIRSALSDAFTTRSRLIRLPESLREKLRSLYQATARLEQRLGRRPTVAELAERMA
                     IPQARVKKLTNVTPEPSPIDELALDHDERWRSRAGRNGDDDPLALALRDELTRQTGEA
                     LARLDPREREVIRLRFGFGHDDELTLAEIGERIGLSRERVRQIERGALLKLHAWALRL
                     GSTAGWRRRASAGVGPKPAGILLAPPAARGRSR"
     gene            complement(6858..7076)
                     /locus_tag="VD788_17580"
     CDS             complement(6858..7076)
                     /locus_tag="VD788_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78126.1"
                     /translation="MLSAEKRLDVTEPGRAGVTLIVALTSMPIDDPTRLDQAAAEVGR
                     VELWFEEEGGEWRLVRAGWSPAGLADLI"
     gene            complement(7340..7684)
                     /locus_tag="VD788_17585"
     CDS             complement(7340..7684)
                     /locus_tag="VD788_17585"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HXV78127.1"
                     /translation="MAHCADCEQVLDEEGRCPDCDDRAEADPVRVTQELVAVLRTSDS
                     NLLPVVKSVLQAAGIPFVVQGDEAMGLLPLGPFGGGVFRDVLGASVLVPQDRADEALE
                     LLRSCDAPNESG"
     gene            7734..8312
                     /gene="cyaB"
                     /locus_tag="VD788_17590"
     CDS             7734..8312
                     /gene="cyaB"
                     /locus_tag="VD788_17590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HXV78128.1"
                     /translation="MSRSTREVEVKLRFDSAEQARSRFEPLGLRLAQARHFEDNVVYD
                     REHEPLVAAGKLLRLRTRGDTSIVTYKAPIAGTHRHKVREEHESIVLDAGAVERILAG
                     LGFRPVYRYQKYRTLYEREGLHLCLDETPIGCFVELEGSPGAIDRAARELGVAPDEYV
                     LLTYRELHERDARERGVEPGDLVFDGGAGNRR"
     gene            8318..9400
                     /locus_tag="VD788_17595"
     CDS             8318..9400
                     /locus_tag="VD788_17595"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HXV78129.1"
                     /translation="MLLSAGLGRRMMPLTLRVPKPAIPVLGRPMAIETLARMARFGVD
                     EVVINLHHLPEAVRRMCSEGLSSAIPEVRFSMEEQILGTAGGLRRAAPLLRGAGPILL
                     HNCDFLSDIDFAAALAAHRHSGRAATLVVVPARPGYGTVEIDRAGRVVSLAGRPAVDP
                     ERVAERMLFTGCHLIEEQVLDMIPDDRPSGIVEQVYRPLAARGALGGYLHPGFWWEFG
                     TPELYLEGSLRLLDLPSVRRIEIASHDPVDEIGSARVAVGAGATLDDGLRLEGRVAVG
                     MASHVAEGTRLADSVVMRESWIGPGSRLERVIVGPGVELPAGYRGGRSLICSADRLPP
                     ESIPVDLRREGDLVVYDFAADRVAGR"
     gene            9397..10392
                     /locus_tag="VD788_17600"
     CDS             9397..10392
                     /locus_tag="VD788_17600"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXV78130.1"
                     /translation="MNDPRDPVRRHLDQRFPGARIERLAGDASSRLFYRVVPADGPSV
                     VVMDYGHPFDGETDDMIVGALFRRAGLRVAALLEASNVNGCLVFEDLGDRDLESTIRA
                     VPPANGAPPPGPLLRAVELAAEVARRGTPVLAASDRAGGPALDAARFRFEMEFFVEHY
                     VEGFLGRVGARERLAPALFDLADRAADGPSMVLCHRDFHSRNLMLPPDGSLAMVDIQD
                     ARWGPDGYDLASILRDAYIDLDEGWVDPLVDRYLDALDAGGDRHAFRRRLRLVAVQRM
                     IKALGSFGYLTSHLATQRYLEPIPRTVRRLAAWLEPTDELRPLREAMRREELLSI"
     gene            complement(10382..11659)
                     /locus_tag="VD788_17605"
     CDS             complement(10382..11659)
                     /locus_tag="VD788_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78131.1"
                     /translation="MFSTTEQLGRARNPNRLLERLSHASARLRERHGVLRLELFHRGS
                     DTVRVTRRRGRRAGLLSFGRDDGVAVRLLRRGEDGIRFAAVSGHDEEAFDEAVRCALD
                     HGAASRTVSDHWADRTDPLLDHATGFGPPSRDELVPWLDRAVRGFVEARCAGDPDVVE
                     QAWVEAAATVETWAVDGRPAASRTRKRGWACLERAAGDGIGWRRPLLTAAARWDEIDG
                     GAWIRFDEQREAPIGLRAAIAGGHRTLVFTPETSATLVSALIRFLAASRAPGRTPVGA
                     GLRVWDAPDDPDALFGGRFDDSGLEARRKLLADGNCLCEPIDGAGHDRRPSFRDPPQP
                     MPSQLLVEPDPVEWPDGSFRVNRLSIQPLDTDRWVLDVGGVVEGGSVSQAFVPTGPSR
                     LVAACSGGWGEAEASHRAVRTPFLVFDGLAVRC"
     gene            complement(11725..13083)
                     /locus_tag="VD788_17610"
     CDS             complement(11725..13083)
                     /locus_tag="VD788_17610"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HXV78132.1"
                     /translation="MIVPVDSATVSWRDALRSAIDPDRTGGRSIQLFLEHREHVRIDF
                     VPGAAPVALRTRISGLTAHDALEYRRQVFRSAPIPEDAAAVAALAAGDRPRGSIADAT
                     SQAPTFPSATRIHETRVIEAMERMARVATAASPAVRLVGRAVASRQHVMIARPDGTDC
                     EETRLSTRVRMMASQRRGPHLATAVVEAALHHRATEFRGETLDRLARALVERVERRLD
                     ARELPGGRHAVVLAPGVGGILVHEIVGHALEADAEHGWLAHAESPVAPPGVDVIDDPR
                     AGRAAWRVDDEGVPSRATPLIRDGRVVGRLHDRLTALRDGVPPTGHGRRSSYREPVMP
                     RMGCTFIASGPRDPDELVRDVGTGVYVRRMEAAHVDSGSGRAIFRVTDADRIVAGRVC
                     APLDPFVMVLDGREVLASLTDVGHDLRFDSCIGSCHREGQSLSMTVGAPTICIGVVGV
                     VG"
     gene            13223..14065
                     /locus_tag="VD788_17615"
     CDS             13223..14065
                     /locus_tag="VD788_17615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV78133.1"
                     /translation="MPNYTILLIDYDPRGIERLRAPLERVGFRVEVATDGPAGIKRFH
                     QLRPDLTLIEAMLPKKHGFDVCRELKSSIHGQNSAVFIVTSVYRGRKYRSEARHLHGC
                     DEYLVRPISDEDLLAAVSRHVSLPDPSRSVDGGGEATEALDPEDAVLSIEAVDDEVPG
                     AGFEVADPDPVEGTSLPPTTPTAGTIDREISDFNLVRLSPGLGADDSTPDPLSSRVVP
                     RMRAGGPAIATARSRPRPARRPRPKPAPPPPQRATSHDQRMVLLAVVLLGLLGLMLAY
                     IVFR"
     gene            14123..14686
                     /locus_tag="VD788_17620"
     CDS             14123..14686
                     /locus_tag="VD788_17620"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXV78134.1"
                     /translation="MAKITKIYTRSGDDGTTGLGTGRRVAKDTARIEAYGAVDELNAA
                     LGVALAVGLDAELERELVSIQNDLFHLGSDLCIPEADKEKTPVPRIEQRHVDALEALL
                     DRLTAEVGPLENFILPGGTVGAAQLHVARTVCRSAERKLVTLVRDEPVGPWTLRYLNR
                     LSDALFVMARFENLRKRVPDLLWDSRR"
     gene            complement(14862..15719)
                     /gene="accD"
                     /locus_tag="VD788_17625"
     CDS             complement(14862..15719)
                     /gene="accD"
                     /locus_tag="VD788_17625"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HXV78135.1"
                     /translation="MAWFKKEKTPKLPQTERRLKVPEGLWVKCAACKELVYKKEVSRN
                     ANVCPKCNYHFRIGSHERLAMLLDDSRFDEFDQDIAPKDALGFVDTKPYAKRIEEYQA
                     KTEMRDAFVSATGTIGGFPVIVGVMEYAFMGGSMGSVVGEKVTRCAERALEERVPLVV
                     VSCSGGARMQEGALSLMQMAKISAALARLDEAGLPFISILTDPTTGGVTASFAMLGDL
                     NLAEPRALIGFAGPRVIEQTIRQTLPEGFQRSEFLLEHGMLDMVVDRARMRDVLIEAF
                     RFMGARNVA"
     gene            complement(15735..>16934)
                     /locus_tag="VD788_17630"
     CDS             complement(15735..>16934)
                     /locus_tag="VD788_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78136.1"
                     /translation="PPAGGGALPGGGAGARHPPAPHDTGSQRGLAAGCLTGLAFLIKG
                     PVGVVLPLLIVLAGRTAAGREILPRPRAALLAVAGWLAVVLPWGLAFVERVGAGAAAA
                     IVRGEALDRFFAGTDHVEPPWFFAAVVAVGFFPWIGPLVVALPRALFQRDDPAARTAL
                     YAAAGLIVGLLFFSIGKSKLASYAVPLAPLAAILAAWELGQELRAPRERTLAPTVLVA
                     LLAAAAVALLIARDVGLPAEAASFAPAGAAVLGVGSLAAMAGLLLRRPRIVWGAAALA
                     STTLLVAATALLLPAIASRRSARALIDGVPALASTERSLVVVDMRVPSLTYYLDRVPE
                     LVPMERLAERIRNGDRPLFVFDEGDLTHAPAAVERLERVGAHGKYVVLREEPSPRVPL
                     DASAPDG"
BASE COUNT         2666 a         5965 c         5882 g         2421 t
ORIGIN      
        1 cgccatcttc gacagaggat cgatctggca caccgccgtg ctgcccgtcg ggggtgatca
       61 cttcaccaac gacctggcgg tcggactgcg cacgccgatc cccgacgccg agcggatcaa
      121 gaagaagcac gggtgcgccc tgtcctcgtt gatccagcag aacgaggccg tcgaggtgcc
      181 gaccgtcggc gggcgaaggc cgcgcttgct gtctcagcag gtgatggccg agatcctgca
      241 gccgcgcgcg gaggagatct tcaccctgtt gcgcgacgag gtgtcgcgcg cgggtttcga
      301 caacttgctc aacgccggcg tggtgctcgc cggcggctgc gtgctgatgc ccggcatgac
      361 cgaggtcgcc gaacaggtct tcgagatgcc ggtgcggcag ggcgagcccg gcggggcggg
      421 gggtctggtc gaccccgcct gcgggcccca gcacacggcg gcgatcggcc tggccgtcta
      481 ctgcgccaag caccgcaagg aagcccgtcg cgcgccgctc ggcgtgccga tcggcccgct
      541 cggtcgcgtc ggtgaccggg ttcgaacgtg gttttccgga attttcttct aacaccggcg
      601 gctgagggag aaagcgatga tcacgatgga tgacaggaac gcacgtgcca ggatcggctt
      661 gacgttcgac gagtcacggg ccagtcaagc gaacatcaag gtggtcgggg tcggcggcgg
      721 cggcggcaac gcggtcagcc gcatgatcgc cgccagggtc gaagggatcc agttcatcgc
      781 ggccaacacc gactgccagg cgctgaggaa caacggcgct ccgatcaagc tgcagctggg
      841 gtccaagctg accaaaggtc tcggtgccgg cggaaacccc gaagtgggca agagcgccgc
      901 actggaggac accgagaaag tgctcgaggc gctgcacggg gccgacatgg tcttcgtcac
      961 caccggcctc ggcggcggca ccgggaccgg ggcggcgccg atcatcgcca acttggcgcg
     1021 cgagatggga gcgctggtcg tcgcggtcgt cacgctgccc ttcgccttcg agggtcggcg
     1081 caggcggacg caggccgagc agggcctgca cacgctgcgc gaggtcgtcg acaccgtgat
     1141 cacgatcccg aacgacaagc tgctgcacac cgtcgagcgc ggcacgccga tcagcgaagc
     1201 gttcaacgtc tgcgacgacg tgctgcgaca ggcggtgcag ggcatctccg acctgatcac
     1261 cgtgcccggc gagatcaatc tcgattttgc cgacgtcaag acggtgatgt cgggcatggg
     1321 catggcgttg atgggaaccg gcgtcgccga gggagagcac cgcgcggtcg aggcggcgca
     1381 acgcgcgatt tcgtcgccgc tgctcgagga tgcgtcgatc cacggcgcgc gcggcgtgct
     1441 gatcaacatc accggcggcg aagacatgtc gctgcacgag gtatcggagg ctgcggcgat
     1501 cgtgcaagag gcggccgatc cggacgccaa catcattttc ggcacggtga tcgacccgta
     1561 tcacgagcac aaggtcaagg tcacggtgat cgccaccggc ttcatgcgcg agcagtcgcg
     1621 cgggctgccg ctgatcgagc ccgtgctaga atcgagctca gcacgaagag aatccacggt
     1681 tccgccgatc gccactccgc ccgaaccgca acaaacagac cggttctttc ggccggcggt
     1741 cggcgagacg ctggatttcg atgcgaacga cgacggcttc acgcccaact tcggcaagat
     1801 gaaggacgac ctcgacgtgc ccgccttcct caggaaacag atggactgat gacgcagcgc
     1861 attctggtca tcgacgacga ccccgggacc tcgacggtcg tccgtagctg gttcaagggc
     1921 gaaccgtacg agatcctcgc cgcggagaat ggcgagcggg gcttgacgat ggcccgcgcg
     1981 gagcgaccgg acgtgatcct gctcgacctc aagatgccgg gaatggacgg catctcggtc
     2041 gcacgcgagc tcaaaaacga ccccctcacg cgcaacacgc cggtgctcgt gctgaccgcc
     2101 tgtcgtgacg tcgacgccaa ggtcgaggcg ttcaacgccg ggacggacga ttacgtcacc
     2161 aagccgttcg agttcgagga ggtcgacgcg cggatccgtt cgatgctgaa gcgccgcgat
     2221 gcgctggtgg ggctggaagc gacggtcgag gagctcaacg acaccaacgc gcagctcgag
     2281 cagctgctga tgatcgacga gaagaccggg ctgtacaatt ttcgcgagtt ccggcaccga
     2341 ctgcgcgccg agtggtcgcg cgccgcacgc tacgagacac cgctgtcgtt ggtattcctc
     2401 gacatcgacg acttcaagaa gctgaacgac acgctgggtc acgcggcggg ggatcgcatc
     2461 ctcgccgagt tcgccacgct ggtcactggc ggagcccgcg ccaacgacgt cgctgcgcgt
     2521 tacggcggcg aggaattctc gatcgtgctt ccgcataccg atggcgagat ggcgctccga
     2581 gtcgccgagc gcatccgccg tgcggtggcc gagttcacct tcatcgcgga cgagcgaccg
     2641 acccggatca ccgtgtcggc cggcgtggcg acctacccct cgaccgcggg tctgtcgacg
     2701 gtcgacgacc tcgtccgcgc cgcggacctg gcgatgtatc gggcgaagga ccgcggcaag
     2761 aactgcgtcg tgcgggcctg acccgttcag cggcgacgcg caacgacggc ggtcgagacc
     2821 aacccgagcg cgagcagccc caccacgctg tcgccgatgc ggtcgtagac cgtgcgctgg
     2881 tcgctgagcc gcacgtcgcg cgcggcgatc gccacctcga acagcccggt gcgctcgtgg
     2941 aaccgcccgc gcgcgtcgat gaagcccgaa attccgctgt tcgccgcgcg cacgaactcg
     3001 ctgcgattct cgattgcacg cagacgtgtg gcgttcgaga gaaaccgctg aaacggcgtg
     3061 cgaccgaacc atgcgtcgtt cgtgatcacg acctggaagc gggcgccggc gtttctcagc
     3121 tcgcgcggca ggtccggaaa gagcagctcg tagcagacca gcacgccggc cggtccggcc
     3181 gcggtctcga gcgtccgcgc gcgcggtccg ggcaggaaac caccggccag ccagcgccac
     3241 tcgccgtcgc gctcctcgac ccacgggccg aagatcgcgc ggaacggcgt ggcctcgacg
     3301 aacgggacga gatagacctt ggccacccag cccggatcca gccgtccgtc cgcgcgtacc
     3361 agcaccgcgg cgttgtaggc gtcgtagtcg tcgcggtcgc ggatcgcgta gtactcgacg
     3421 ccgacgagga actcgctgtc gagctcacgc gccaactgct gcacctcggg catcgcgtag
     3481 gtcgccggcg cgtcgagcca atgctcgagc gggtcgggtc gcgcgctctc gggccagatc
     3541 accagctgcg ggtcgagcgc cgcggcatcg cgtgacagcg cggccagcct gctccgctgc
     3601 tcggcctcgg tgccttcgcc gcgtttgacc agcagcggga tgtcgggttg gatgtagacg
     3661 acacgcaccg tctcccgttc gggcagcggg ccgaaccagc accacgcgtc gtacgccagc
     3721 accgccgccg acaggcagcc gagcgcgatc gcggcgccgc ggcgcgatcg gcgtcgttcg
     3781 gcgagcatcg cgtcgtaggc caggccgttg accacgtaga ccaacgcgct gacgccgtag
     3841 tggccgatca ggtcggcgaa ctggatcagg aacgggtagg ccgccatcgc gtgcgcggcg
     3901 tggtcgccgg tcatccgcag gtcaccgaag gtcagcagcc agtcgagcgg gacccagacg
     3961 atcggcagcg tccagcccca actgaagccg gtgcgccgcc gcagccacga ggcgaccatc
     4021 agacacgagg agagcttcag cgccagcagc ccggcgaacc agacccacag caccgccgcc
     4081 agccacgaga actcgagcat cgcgtagcag aagtgcaggc cgatgatcgc ggcggcgaag
     4141 ccgaaaacga agctgccggt gaagcggtac cagcgagacc gctcgccgtt ccggtcgagc
     4201 cagtacaaca cgggcgtcag cgccagcaac aacggcagca acagctcggt gtaatagccg
     4261 acaccgagcg cgacgccggc gatcagcggt agcgtgaagc gatgggttgc ccaacgatgc
     4321 aaggcgggct aacgcgtcag gcgccgtccc gcgccagtcg gatcccgctg aactgccagc
     4381 gggcgtcggg atagaagaag ttgcggtagc tcgtgcgcag gtgcgagcgc gggctggcgc
     4441 acgagccgcc gcgcagcacg agctggttgc acatgaactt gccgttgtat tccccgagcg
     4501 cgccggcgag cggtcgatag ccgggatagc cgacgtacgg gctggccgtc cattcccaca
     4561 cgttgcccat catctgttcg agccgcgcgc cgccagcggc ggccggtacc gggtgcagcc
     4621 ggccgtcgtc gaggaagttg ccgtcgacgg cgagcccgga tgcggcgacc tcccactctg
     4681 cctcggtcgg caaccgatga ccgcgccagc gcgcgtaggc ctcggcctcg tagtagctca
     4741 cgtggcacac cggctcgtcg agccgtagct ccttcagccc cgacgcggtg aaggtcatgc
     4801 gccgccccgc gctcccgagc cagtaggccg gatgccgcca accctcgcgt tgcgccacgg
     4861 cccatccgtc ggccagccac agccccggct cgagatagcc gccgtcgtcg atgaactcga
     4921 ggtactcggc gttggtcacc ggccgcgagg ccagccggaa cacctcgagc cagacgcgat
     4981 ggcgcggcgt ttcgttgtcg aagcagaagc cctcgccgtc gtagccgatg tggcgcacgc
     5041 cctcgtcgaa cgcgacccac tcgatcggac ccggacgggc gaccgggctc tcctcacacc
     5101 ggcgcaggac cggatgggtc gggttccgtg agagcaggtg cttgacgtcg gtgacgagca
     5161 gctcttggtg ctgctgctcg tggtggattc cgacctcgac cagccccgcg acgtcgaccg
     5221 tatcgcgggc gagccccggc tcggcgagac accgtcggat ctgatcgtcg acgtggcgtc
     5281 gataggccag cacgcggtcg agcgacggac gcgtcagcag accgcgatcg gcgcgggagt
     5341 actgttcgcc gatcgtgttg tagtacgagt tgaacaacac gcgaaagtcg gcctcgaacg
     5401 ggcggaagcc aggcatcccg gcttcgagaa cgaacgtctc gaagaaccac gtggtgtggg
     5461 ccaggtgcca cttggctggg ctcgccagcg gcatcgactg cacgacgcag tcctccgccg
     5521 acagcccgtc gatcaacgcg agcgacgtgt gccggacttc gtcgtagcga ccggccagct
     5581 cgtcccggcc gcgccggggt cggggggtgc tgcggtcggt cgcggtcgtg ctcatctcga
     5641 gcgtcctcgt gcagcgggcg gagcgagcag tataccagcc ggcttcggtc cgaccccggc
     5701 cgaggcacgc cggcgccaac cggcggtcga gccgaggcgc aaggcccagg cgtgcagctt
     5761 cagcagtgcg ccgcgctcga tctgacggac ccgctcgcgg ctcagcccga tccgttcgcc
     5821 gatctcggcc aacgtcagct cgtcgtcgtg accgaagccg aagcgcaggc ggatcacctc
     5881 ccgctcgcgc gggtcgagcc gagccagtgc ctcgccggtt tgtcgcgtca actcgtcgcg
     5941 cagggcgagc gcgagcggat cgtcgtcccc gttccgcccg gcgcgtgagc gccagcgctc
     6001 gtcgtggtcc agtgccagct cgtcgatcgg gctcggctcg ggcgtgacgt tagtcagctt
     6061 cttcacccgc gcttgcggaa tcgccatccg ttcggccaac tcggccaccg tcggccgccg
     6121 tccgagccgc tgctcgagcc gcgcggtcgc ctggtacaac gaccgcaact tctcccgcaa
     6181 gctctccggc agacggatca gccgcgagcg agtggtgaag gcatcggaca gcgcgctacg
     6241 gatccaccac gaggcgtagg tcgagaagcg gtagccgcgg tcggggtcga agcggtcgac
     6301 cgccttgatc agaccgacgt ggccctcctg tacgagatcg gcgaacggga tcacgccgcg
     6361 atcgtagcgc cggacgatcg cgggcacgag cgccaagttg ccgaccacca gtgcctcgcg
     6421 cgcgcggtgg aggcgacgcc tgagctcgcg ggcccgctcg aagagtgggg tgatcgcgtc
     6481 gtcgtgtcgc gccgccccga gccgtgccag tcggaggtag caggcctcga gatagtcgat
     6541 cggccagtga ccgtcgcgct cgggtggcac gccgtcgagc acgatggtgc gcgtcgtgct
     6601 cggcagctcg ccgagcacct cgatcagctg gcggcgcgca tcgcgcaggt cacgtgcgtg
     6661 ctccaactcc cgttcgcggg cgtcctgcgg ctcccgtacg cgcgtgagct cgtcaagctc
     6721 cgagatcgac gtcattccgc cctcggttct ggtgcctggt gcgaccctgg aatgatcgta
     6781 ggcgcggcat gcggcgcgcg cagtggcgtt gcgcgtcaac ttcccggtaa agcgcgccac
     6841 ggcgccccgg ggaggggtca gatcaggtcg gccaggccgg ccggggacca gccggcgcgg
     6901 acgagccgcc actcgccgcc ttcctcttcg aaccacagct cgacgcgacc gacctcggcc
     6961 gcggcctggt cgagacgggt cggatcgtcg atcggcatcg acgtcagcgc gacgatcagc
     7021 gtcacgccgg cgcgccccgg ctcggtgacg tcgagcctct tctccgccga cagcacgaag
     7081 accggtccgc tgcggaagag gtagccgcgg acgagatcga gcagccgatc gccgtccctc
     7141 ccggcggcgt cggcgtagcg tggcgagagg aagtcgcgca gccgcgcgta gtctttgctt
     7201 tcgatcgcgg cctcgccgtc tgcgatcgtg cggcgcaggc gatcctcggg tgactccgac
     7261 tcgcacgagg cgagcgcgac gagcgcggcg gtgagggccg cacgccggat gctgcggccg
     7321 gctgcgcccc cgcccgggct cagccgctct cgttcggggc gtcgcagctc cgcaacagct
     7381 cgagcgcttc gtcggcccgg tcctgcggca ccagcaccga tgcgccgagc acgtcgcgga
     7441 agacgccgcc accgaaaggc ccgagcggca gcagtcccat cgcttcgtcg ccctgcacga
     7501 cgaacgggat ccccgccgcc tgcagcacgc ttttcaccac cggcagcagg ttgctgtccg
     7561 acgtgcggag caccgcgacg agctcttgcg tgacgcgcac cggatcggct tccgctcgat
     7621 cgtcgcagtc ggggcatcgg ccttcctcgt cgagcacctg ctcgcagtcc gcgcaatgtg
     7681 ccattgcccg cgctctctcc gtcgacgtaa cgggcgatgc tatcatggcg ggaatgtcgc
     7741 gatccacacg cgaggtcgag gtcaagctcc ggttcgactc cgccgagcag gcgcgaagcc
     7801 gcttcgagcc gctcggtctg cgtttggcgc aggcccgcca cttcgaggac aacgtcgtct
     7861 acgaccgcga acacgagccg ctcgtcgcgg ccggcaagct gctccggctg cgcacgcgcg
     7921 gcgacacgtc gatcgtcacc tacaaggcgc cgatcgcagg cacccaccgg cacaaggtcc
     7981 gggaagagca cgagtcgatc gtgctcgacg cgggtgcggt cgagcgaatc ctggcggggc
     8041 tcggcttccg cccggtctat cgttatcaga agtaccgcac gctctacgag cgagaaggtc
     8101 tgcacctctg cctcgacgag acgccgatcg gctgtttcgt cgagctcgag gggtcgccgg
     8161 gggcgatcga tcgcgcggcc cgggagttgg gcgtcgcccc cgacgagtac gtcctgctga
     8221 cctaccgcga gctgcacgag cgggacgcgc gcgagcgcgg cgtcgagccc ggcgacctgg
     8281 tcttcgacgg cggcgcgggg aaccggcggt gaaggccatg ttgctgtccg ccggcctcgg
     8341 ccggcggatg atgccgttga cgctgcgcgt gccgaagccg gccattcccg tgctcggtcg
     8401 gccgatggcg atcgagacgt tggcacggat ggcgcgtttc ggcgtcgacg aagtcgtgat
     8461 caacctgcac catctgccgg aggcggtgcg ccggatgtgc tcggagggtc tttcctcggc
     8521 gatccccgag gttcggttct cgatggagga gcagatcctc ggcacggccg gcggcctccg
     8581 ccgtgccgct cctctgctgc gcggggccgg tccgatcctg ttgcacaact gcgatttcct
     8641 gtcggacatc gattttgccg cggcgctcgc cgcccatcgc cactcgggca gggcggccac
     8701 gctggtcgtc gtgccggccc gccccggcta cggcacggtc gagatcgatc gcgcggggcg
     8761 ggtcgtctcg ctggcggggc gacccgcggt cgaccccgaa cgcgtcgccg aacggatgct
     8821 gttcaccggc tgccacctga tcgaggaaca ggtcctcgac atgattcccg acgatcgccc
     8881 gagcgggatc gtcgagcagg tctatcggcc gctcgccgcc cggggggctc tcggcggcta
     8941 cctccatccc gggttctggt gggagttcgg cacgcccgag ctctatctcg aaggctcgct
     9001 gcggttgctc gacctgccca gcgtccgtcg aatcgagatc gcctcgcacg atccggtcga
     9061 cgagatcggc agcgcgcgcg tcgccgtcgg cgcgggggcg accctcgacg acgggctgcg
     9121 gctcgagggc cgagtggcgg tcgggatggc gagccacgtt gccgagggca cgcgtcttgc
     9181 ggacagcgtg gtgatgcggg agtcgtggat cggaccgggg agccgcctcg agcgcgtgat
     9241 cgtcggaccg ggtgtcgagc ttcccgccgg ttaccggggc ggacgctccc tcatctgtag
     9301 cgccgaccgg ctgccacccg agtcgatccc ggtcgacctt cggcgggaag gcgacctggt
     9361 ggtctacgac ttcgcggccg accgggtcgc cggtcgatga acgatcctcg cgacccggtc
     9421 cgacggcacc tcgatcagcg ctttccgggt gcccggatcg agcggctcgc gggcgacgcc
     9481 tcctcgcgtc tgttctaccg ggtcgtcccc gccgacgggc cgtccgtcgt cgtgatggac
     9541 tacggccacc cgttcgacgg cgaaacggac gacatgatcg tcggcgcgct cttccgccgg
     9601 gcgggattgc gcgtcgcggc cctgctcgag gcgtcgaacg tcaacggctg cctcgtcttc
     9661 gaggacctcg gcgatcgcga tctcgaatcg acgatccggg cggttccgcc cgcgaacggc
     9721 gctccacccc cggggccgct cctgcgtgcc gtcgagctcg cggcggaggt ggcgcggcgc
     9781 ggcaccccgg tcttggcggc gtcggaccgc gccggcggcc cggcactcga cgcggcgcgc
     9841 ttccgcttcg aaatggagtt cttcgtcgag cactacgtcg agggtttcct cggccgggtc
     9901 ggggcgcgcg agcggctcgc ccctgcgctg ttcgacctgg ccgaccgggc cgcggacggg
     9961 ccgtccatgg tgctctgcca tcgagacttc cacagccgca atctgatgct gccccccgac
    10021 ggctcgctgg cgatggtcga catccaggac gcgcgttggg gaccggacgg ctacgacctg
    10081 gcttcgatcc tgcgcgacgc ctacatcgat ctcgacgagg gctgggtcga cccgctcgtc
    10141 gaccggtact tggacgctct cgacgccggt ggcgatcgcc acgccttccg ccgccgactc
    10201 cgactcgtcg ccgtgcagcg gatgatcaag gccctcggta gcttcggcta tctgacgtcg
    10261 catctcgcga cgcagcgcta tctcgaaccg atcccgcgaa cggtccgacg cctcgccgcg
    10321 tggctcgagc cgacggacga gctgcgcccg ctccgcgagg cgatgcgtcg cgaggagttg
    10381 ctcagcatct gaccgcgagc ccgtcgaaga ccagaaacgg cgtacgcacg gctcgatggg
    10441 acgcttccgc ctcgccccaa ccgccggagc atgccgcgac cagacgggag ggtccggtcg
    10501 gcacgaacgc ctgggacacg ctcccgccct cgacgacacc gcccacgtcg agcacccagc
    10561 gatcggtgtc gagcggctgg atcgacaggc ggttgacccg gaacgacccg tcgggccatt
    10621 cgacgggatc gggctcgacg agcagttggg acggcatcgg ctgcggagga tctcggaagg
    10681 atgggcgacg gtcgtggccc gccccgtcga tcggctcgca gagacagttc ccgtcggcga
    10741 gcagcttccg ccgcgcttcg aggccgctgt cgtcgaatcg cccgccgaac aacgcgtccg
    10801 ggtcgtcggg ggcgtcccag acgcggagac cggcgccgac cggggtccgg cccggcgccc
    10861 gcgaggcggc gagaaaacgg atcagggccg agaccagcgt ggcggaggtc tcgggtgtga
    10921 acaccagcgt acggtgcccg ccggcgatcg ccgcacgcag gccgatcggg gcctcgcgct
    10981 gctcgtcgaa acgaatccac gcgccgccgt cgatctcgtc ccaccgcgcc gcggccgtca
    11041 gcagcgggcg ccgccagccg atgccgtcgc cggccgcccg ctcgaggcaa gcccagccgc
    11101 gcttccgcgt ccgcgaagcc gccggccggc cgtcgacggc ccacgtctcg accgtcgccg
    11161 ccgcctcgac ccaagcctgc tcgacgacgt ccggatctcc cgcgcacctc gcctcgacga
    11221 acccgcgcac cgcgcgatcg agccacggca cgagttcgtc ccgcgacggg ggaccgaagc
    11281 ccgtcgcgtg gtcgagcagc gggtcggtcc tgtcggccca gtggtcggag accgttcggc
    11341 tggcggcgcc gtggtcgagc gcacagcgga cggcctcgtc gaacgcctcc tcgtcgtggc
    11401 cggatacggc cgcgaagcgg atgccgtcct cgcctcgccg aagcagacgg acggcgacgc
    11461 cgtcgtcccg gccgaacgag aggaggccgg cgcgacgacc acgccggcgc gtaacgcgaa
    11521 cggtgtccga gccgcggtgg aacaactcga gtcgtaggac accgtgccgc tcgcgcaagc
    11581 gcgccgaggc gtgggacagt cgctcgagca atcggttcgg attgcgagcg cgaccgagtt
    11641 gttccgtagt gctaaacacc gtggtaggtc aaccggtccg ctgaccctgg tcctctgtcc
    11701 aacccttcat cgaacggcac gaattcatcc caccaccccg accaccccta tgcagattgt
    11761 cggcgcgccg accgtcatgg acaggctctg cccctcccga tgacacgagc cgatacagct
    11821 gtcgaagcga aggtcgtggc ccacgtcggt tagcgacgcg agcacctcgc gtccgtcgag
    11881 caccatgacg aacggatcga gtggagcgca cacgcgaccc gcgacgatac gatcggcgtc
    11941 ggtcacacgg aagatggcgc gaccgctccc gctgtcgacg tgggcggctt ccatgcggcg
    12001 gacgtagaca cccgtaccga catcgcgaac gagctcgtcg gggtcgcgcg gcccggacgc
    12061 gatgaaggta caccccatgc gaggcatcac cggctcgcgg taggacgagc gccgcccgtg
    12121 gccggtcggc gggacgccgt cgcggagcgc ggtcagccgg tcgtggagtc gtccgacgac
    12181 gcgcccgtcc cggatcaacg gcgtcgcccg gctcggcacg ccctcgtcgt cgaccctcca
    12241 ggccgcgcga ccggcgcgcg ggtcgtcgat cacgtcgacc cccggcgggg cgaccgggct
    12301 ctccgcgtgc gcgagccagc cgtgctcggc gtcggcctcg agcgcgtgac cgacgatctc
    12361 gtgcaccagg atgccgccga cgccgggagc caggacgacc gcgtgacggc cgcccggaag
    12421 ctcccgggcg tccagtcgcc gctcgacccg ctcgaccaac gcgcgggcca gccggtcgag
    12481 ggtctcgccc cgaaactcgg tcgcccgatg gtgcagcgcg gcctcgacca ccgctgtcgc
    12541 cagatgcggg cctcgtcgct ggctggccat catgcggacg cgggtcgaga ggcgggtctc
    12601 ctcgcagtcg gtgccgtccg gacgcgcgat catcacgtgc tggcgcgagg cgaccgctcg
    12661 gccgaccagg cgcaccgccg gcgaggcggc cgtagcgaca cgggccatcc gttccatcgc
    12721 ttcgatcacc cgcgtctcgt ggatccgagt ggccgacggg aatgtcggcg cttgggaggt
    12781 cgcgtccgcg atcgaccccc gcgggcgatc gccggccgcc agagccgcca ccgcggcggc
    12841 gtcttccggt atcggagcgg agcggaagac ctgccgacgg tactcgagcg cgtcatgtgc
    12901 cgtcaggccc gagatccggg tgcgcagggc gaccggggcc gcgcccggca cgaagtcgat
    12961 ccggacgtgc tcgcgatgct cgaggaagag ttggatcgag cgcccgccgg tccggtcggg
    13021 gtcgatcgcc gaccgaagcg cgtcgcgcca cgagacggtt gcggagtcga cagggacaat
    13081 caacgctgat ggcccaccgg gcacgacttg aaagcgcgtt cggacgcggc tatggtgcta
    13141 ggcgttcgcg gcgatccacg cgggttttcg gccgaaagac ggcgtagaat tcagacgccg
    13201 cacccgcggc gccggggctt ccatgccgaa ttatacgata ctgctgatcg actacgaccc
    13261 ccgcggtatc gaaaggctcc gggcaccgct cgaacgcgtc ggcttccgag tcgaggtcgc
    13321 gaccgacggg ccggctggaa tcaagcggtt ccaccagctt cgccccgatc tgaccctgat
    13381 cgaggcgatg ctccccaaaa aacacgggtt cgacgtctgc cgcgagctga aatcgagcat
    13441 ccacggccaa aactcggctg tgttcatcgt gacatcggtc taccgggggc gaaagtaccg
    13501 tagcgaggcg cgccacctgc acggttgcga cgagtatctc gtgcggccga tctcggacga
    13561 ggatctgctg gcggccgtct cgcgtcacgt cagcctgccc gacccgtcga ggtccgtcga
    13621 cggcggcggc gaagccaccg aggccctgga tccggaggac gcggtcctgt cgatcgaagc
    13681 ggtcgacgac gaggtccccg gcgccgggtt cgaggtcgcg gatccggatc cggtggaagg
    13741 cacctcgctc cccccgacga ccccaacggc cgggacgatc gaccgcgaga tctcggattt
    13801 caacctcgtg cggctctcgc cgggccttgg agccgacgat tcgacccccg accccctttc
    13861 ctcccgggtc gtaccgcgca tgcgggccgg gggaccggcg atcgccacgg cccgttcgcg
    13921 tccgcgtccg gccagacgcc cgcgaccgaa acccgctccg ccgccgccac agcgggccac
    13981 ctcccacgac cagcggatgg tgctgctggc ggtggtgctg ctggggctgt tggggttgat
    14041 gctggcctat atcgtgttcc gctgacgctc ggtggctccg cggtagactg atgccgaccc
    14101 cgagcacgga ggaagcgccg cgatggccaa gatcacgaag atctacacgc gctccggcga
    14161 cgacggaacg accgggctcg gcacgggccg acgcgttgcc aaggacaccg cccgtatcga
    14221 ggcctacggc gcggtcgacg agctgaacgc cgccctcggc gtcgcgctgg ccgtcggtct
    14281 cgacgccgag ctcgagcgcg agctggtgtc gatccaaaac gacctgttcc atctcggctc
    14341 cgacctgtgc atcccggaag cggacaagga aaaaacgccg gtaccgcgga tcgaacagcg
    14401 gcacgtcgac gcgctcgagg cgctgctcga tcggttgacc gccgaggtcg gcccgctcga
    14461 gaacttcatc ctgccgggtg ggacggtcgg cgccgcgcag ctgcacgtcg cccggaccgt
    14521 ttgccggtcg gcggaacgaa agctggtgac gctcgtgcgc gacgagccgg tcgggccgtg
    14581 gacgctgcgt tatctcaacc ggctgtcgga cgccctgttc gtgatggccc gcttcgagaa
    14641 cctgcggaag cgggttcccg atctcctgtg ggatagccga cgctagcacg gcgaaccaag
    14701 gataaggcgt ccccggcgtc ggaggagggg ctttggactc gtcgcgccgg gaacgcctcg
    14761 tctcggtccg agagggcagc ggcctctctc actactcacc tacgctcgag ccgtcgagtg
    14821 gttgcactcc acccgggtaa aaaagtgtaa cgccgagccg gctaggcgac gttgcgcgca
    14881 cccatgaagc ggaaggcctc gatcagcacg tcccgcatgc gggcgcggtc gaccaccatg
    14941 tcgagcatgc cgtgctcgag caggaattcg ctgcgctgga acccttcggg caacgtctga
    15001 cggatcgtct gctcgatcac gcgcggcccg gcgaaaccga tcagcgcgcg cggctcggcg
    15061 aggttcaggt cgcccaacat ggcgaacgaa gccgtgaccc cgccggtcgt cgggtcggtc
    15121 aggatcgaga tgaaggggag gccggcctcg tcgagacgcg cgagcgcggc ggagatcttc
    15181 gccatctgca tcagcgacag cgcgccttcc tgcatacgcg cgccgcccga gcaactgacg
    15241 accacgagcg ggacccgttc ctcgagcgcc cgctcggcgc aacgcgtgac cttctcgccg
    15301 acgacgcttc ccatcgatcc gcccatgaag gcgtactcca tcacgccgac gatcaccgga
    15361 aagccgccga tcgtccccgt cgccgagacg aatgcgtcac gcatctcggt cttggcttga
    15421 tactcctcga tccgcttggc gtacggcttg gtgtcgacga agcccaaggc gtccttcggc
    15481 gcgatgtcct gatcgaactc gtcgaaccgg ctgtcgtcga gcagcatcgc cagtcgctcg
    15541 tgggagccga tgcggaagtg gtaattgcac ttggggcaga cgttcgcgtt gcgcgagact
    15601 tccttcttgt agaccagctc cttgcaggcc gcgcacttga cccacaaacc ttccggcacc
    15661 ttgagccgtc gctcggtctg cggcagcttg ggcgtctttt ccttcttgaa ccaggccatc
    15721 gtcttcgccc caacctaccc atccggcgcc gacgcgtcaa gcgggactcg cggcgacggt
    15781 tcctcgcgga gcacgacgta cttcccgtgg gccccgacgc gttcgagccg ctcgaccgcg
    15841 gccggcgcgt gggtcaggtc gccctcgtcg aatacgaaca gcggccggtc gccgtttcga
    15901 atgcgctcgg cgagccgttc catcggcacg agctccggga cgcggtcgag ataataggtc
    15961 aggctcggta cccgcatatc gaccacgacc agcgatcgct cggtcgacgc gagcgccggg
    16021 acgccgtcga tcagcgcgcg cgccgagcgg cgggaggcga tcgcgggcag cagcagcgcg
    16081 gtcgccgcca cgagcaaggt cgtgctcgcg agcgccgccg cgccccacac gatgcgcggg
    16141 cgacgcaaca gcagaccggc catcgcggcg agcgatccga cgccgaggac ggcggctccg
    16201 gccggcgcga agctcgccgc ctcggccggt agaccgacgt ccctcgcaat cagcaatgcg
    16261 acggcggccg cggcgagcag tgccacgagc accgtcggcg ccagcgtgcg ctcgcggggg
    16321 gcgcgcagct cctgaccgag ctcccacgcg gcgaggatcg ccgccagcgg cgcgagcggc
    16381 accgcgtagc tggccagctt gctcttgccg atcgagaaga acagcagccc cacgatcagg
    16441 cccgccgcgg cgtagagcgc cgtgcgcgca gccggatcgt cccgctggaa cagtgcgcgc
    16501 ggcagggcga cgaccagcgg tccgatccac gggaagaagc cgacggcgac gaccgcggcg
    16561 aaaaaccacg gcggctcgac gtgatccgtt ccggcgaaga accgatccag cgcctcgccc
    16621 cgcacgatcg ccgcggcggc gcccgcgccg acgcgctcga cgaaggccag accccacggc
    16681 aggacgacgg ccagccagcc cgcgaccgcg agcaacgccg cgcgcggtcg cggcaggatc
    16741 tcccggcccg ccgcggtccg accggcgagc acgatcagga gcggtagcac cacaccgacc
    16801 ggtcccttga tcagaaaggc cagaccggtc agacagcccg ccgccaggcc gcgctgggag
    16861 ccggtgtcgt ggggcgcggg ggggtggcgc gccccggccc ccccccccgg cagcgccccc
    16921 ccaccggcgg gggg
//
LOCUS       DASPGN010000606         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_933873, whole genome shotgun sequence.
ACCESSION   DASPGN010000606 DASPGN010000000
VERSION     DASPGN010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933873"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..922
                     /locus_tag="VD788_17635"
     CDS             92..922
                     /locus_tag="VD788_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78137.1"
                     /translation="MMYNRSMAVLAGAALGAAVFACSDESSGGGIGLAFSTRPAPTGA
                     LGGAMSAGAMQLGDTVVLNGDSIILETVEVVLREVEIEGEDVADCSSSSSSDDDGCEE
                     FSYGIQLVSLPLGSETEKVVTLNGVPPGMYDEVEFDIHQPVSPGDDAFIAANPLFDGV
                     SIRVTGFYRAAGASERTAFTYTTDLSQEQEIELSPPLDVTADGLANITIRIDVSTWFL
                     NGSGTGIVDPATAAPGGANESLVEGNIEQSIDAFQDDDSDGLDDDDEDDDGGDEHDDS
                     "
     gene            1148..1996
                     /locus_tag="VD788_17640"
     CDS             1148..1996
                     /locus_tag="VD788_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78138.1"
                     /translation="MRGKRVVLEIVGMAAAAAAAACSDSSGGAGVGLAFSTRPAPTSV
                     MASYVGTGGGMHASVVQAGDSTVVALGNDTVIIRSVELVLREIELERVDAPADCDSTA
                     VGQDDACEEFTVGVQLVALPLGNSTDKVISIDAPAGLYDEIEFDIHKPESSNDAAFIA
                     ANPDFDGVSIRVTGTYSQAGSRSDFVYTTDLDKEQERALIPPLDVTAGGPVNVTLRVD
                     ISSWFLTETGLSLVDPATANKGGQNEGVVKNNIEQSIEAFHDDDSDGLDDDSEDDDGG
                     TSPDGL"
     gene            2106..>2773
                     /locus_tag="VD788_17645"
     CDS             2106..>2773
                     /locus_tag="VD788_17645"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXV78139.1"
                     /translation="MSAVWIDGQFYDRDHATISVFDHGLLYGDGVFEGVRAYGGGVFR
                     LERHIERLYHSAKAIWLEVPMSRAEMAEVVRAAVAQSGLQDAYIRLVVTRGKGDLGLD
                     PRKCPRPSVICIVDRIVLWPADRYEKGLTCLTSPTPINHRESLSPRVKSLNYLSHILA
                     KIEGIAAGVDEVLMLDASGYVAEASGMNLFVVTDRVLSTPPPYAGILKGVTRDAVIEL
                     APAAG"
BASE COUNT          449 a          932 c          982 g          410 t
ORIGIN      
        1 tgtcagccaa gccgagcgga aaccgcgggg acctcgcggg accctcgccg ggtaccttgc
       61 tccgaagacg tacgactcga gcaaggaggc gatgatgtac aacaggtcca tggcggtgct
      121 ggccggagcg gcgctcggcg ccgctgtctt cgcgtgctcc gacgagagca gcggcggcgg
      181 catcggtctc gcgttctcga cgcgaccggc cccgaccggc gcgttggggg gcgcgatgag
      241 cgcgggcgcg atgcagctcg gcgacacggt ggtgctgaac ggcgattcga tcatcctcga
      301 aacggtcgaa gtcgtgctcc gcgaggtgga gatcgagggt gaggacgtcg ccgactgctc
      361 gtcctcgagc agctccgatg acgacggctg cgaggagttc tcctacggca tccagctcgt
      421 gtcgctgccg ctcggcagcg agacggagaa ggtcgtcacg ctgaacggcg tgccgccggg
      481 gatgtacgac gaagtcgagt tcgacatcca ccagccggtg agcccgggcg acgacgcctt
      541 catcgcggcc aacccgctgt tcgacggcgt ctcgatccgc gtcaccgggt tctaccgcgc
      601 cgccggcgcg tccgagcgga ccgcgttcac ctacacgacc gacctcagcc aggagcagga
      661 aatcgagctg tcgccgccgc tcgacgtgac cgctgacggc ctcgccaaca tcacgatccg
      721 catcgacgtc tcgacctggt tcctcaacgg gagcgggacc gggatcgtcg acccggcgac
      781 ggcggcgccc ggtggcgcca acgagagcct ggtcgagggc aacatcgagc agtcgatcga
      841 cgcgttccag gacgacgact ccgacgggct cgacgatgac gacgaggacg acgacggggg
      901 cgacgagcac gacgattcct gaagcgcgct ctgacggtgg cgcccggcat cgcaccggac
      961 gccgccgttc tccctgcctc gttttgagcc agcggtactg cggcgaagcg gtctccgacc
     1021 tccgcccccg cgcataacct ccttccggac aagcgattac acgcctggcc cggccgagta
     1081 cgccccttgc gtttgagccg ggcagcgcgc gaacggcgcg cacctactca actgcaccgg
     1141 aggcggtatg cgcggcaagc gtgtggttct ggagatcgtc ggaatggcgg ccgcggcggc
     1201 cgcagcggca tgctccgatt cgagcggggg cgcaggcgtc ggcctcgcct tctcgacccg
     1261 accggccccg acgagcgtga tggcgtccta tgtggggacc ggcggcggca tgcacgcgtc
     1321 ggtcgtacag gccggggact cgacggtcgt ggcgctcggc aacgacacgg tcatcatccg
     1381 cagcgtggag ctggtgctgc gcgagatcga gctcgagcgc gtggacgcgc ccgcggactg
     1441 cgattcgacc gcagtcggcc aggacgatgc ctgcgaggag ttcacggtcg gcgtccagct
     1501 cgtggcgctg ccgctcggca actcgacgga caaggtcatc tcgatcgacg cgcccgcggg
     1561 gctgtacgac gagatcgagt tcgacatcca caagccggag agctccaacg acgccgcatt
     1621 catcgccgcg aacccggact tcgacggtgt ctcgatccgg gtgaccggga cgtacagcca
     1681 ggcggggtcg cgcagcgact tcgtctacac gaccgacctc gacaaggagc aggagcgggc
     1741 cctgatcccg cccctcgacg tgacggcggg cgggccggtg aacgtgaccc tgcgggtcga
     1801 catctcgagc tggttcttga cggagaccgg cctctccctt gttgatccgg cgacggcgaa
     1861 caagggcggc cagaacgagg gggtcgtgaa gaacaacatc gagcagtcga tcgaggcgtt
     1921 ccacgacgac gactcggacg gcctggacga cgactccgag gacgacgacg ggggcaccag
     1981 cccggacggc ctctaggagc ggttgtcacg gagggccgcc gcggcgagat tgcggcggcg
     2041 cgggggcggg tcctcgggcc cgcccccgtg tttttgcgtt catccgacac gcgagggagg
     2101 ccgctgtgag cgctgtgtgg atcgacgggc agttctacga ccgcgatcac gctaccatct
     2161 ccgtgttcga ccatggcctg ctgtacggcg acggtgtctt cgaaggcgtc cgcgcgtacg
     2221 ggggcggtgt gttccgcctc gagcggcaca tcgagcgcct gtaccactcg gcgaaggcga
     2281 tctggctcga agtgcccatg agccgggccg agatggccga ggtcgtgcgg gcggcggtgg
     2341 cccaaagcgg tctccaggac gcctacatcc gcctggtcgt gacccgcggc aagggcgatc
     2401 tcgggctcga cccgcgcaag tgcccgcggc catccgtcat ctgtatcgtg gaccggatcg
     2461 tcctctggcc ggcggaccgc tacgagaagg gcctgacctg tctcacgtcc cccacgccga
     2521 tcaaccaccg cgagagcctg tcgccccggg tcaagtcgct caactacctg tcccacatcc
     2581 tcgccaagat cgaggggatc gcggccggcg tggacgaggt gctgatgctc gacgcgtcgg
     2641 gctacgtcgc tgaggcgagc gggatgaacc tgttcgtggt cactgaccgg gtcctcagca
     2701 ccccgccgcc gtacgccggg atcctcaagg gcgtcacgcg cgatgcggtg atcgagctcg
     2761 cgccggcggc ggg
//
LOCUS       DASPGN010000607         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_817095, whole genome shotgun sequence.
ACCESSION   DASPGN010000607 DASPGN010000000
VERSION     DASPGN010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817095"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2508)
                     /locus_tag="VD788_17650"
                     /pseudo
     CDS             complement(1..2508)
                     /locus_tag="VD788_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263577181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
BASE COUNT          406 a         1004 c         1046 g          515 t
ORIGIN      
        1 gtcaagtcgg ggatctcgaa gcgggcgcgc gtgtagatcg acgaggtgcc ggcgggcacg
       61 aaggtgtcga tcagatcggt cgcgccgccc acctcgagct cgtagccgat gccgtagttg
      121 cccttgggcc acgacgagtc gtcgaaggcc ggctggaacc agctggtgtc gacaccggga
      181 ttcgaggtgt tggccagata ggtgatcgtc gtcgaccggt tgatgctgac cttgggcacc
      241 agcaccagat cggacgacgg cggggtcggc gggatccggt tccagacgcc gatcgcgacg
      301 acgttcgttc cggtctgcag cgcaggcagc gccgcggccg agacgtccac cggcaggccg
      361 tagttcggcg tggcctggtt gctcgcttcg tgggccgacg gactggcgtc gtagggcacg
      421 gggctgccga cgggcatctc gggggaacga taaacctcct cgccgttgac ccacaccacg
      481 taaccgtcgt cgtaatccgc ggcaacccac atgttctcga ccgccgcggc gtccggcacg
      541 tcgaacgtcg cccgggtgta gaccgacgcg gcgccgacct cgacttcggt ctgcagcagc
      601 gcctcggccc cggtcgtcgc ctcgtagccg acgccgtagc tgccgagcga ccaaccgctg
      661 tcgtcgaacc cgggagcggt ccagccgtca cccagcaccg gatcggtcat gttgaccaga
      721 taggtcatcg gcatgcccgc cgcgaccacg accgtctcga ccgggctcat caagtcgacg
      781 aggtccatgt cgacgacggc gcacccgccg gcgcagaccc cgtcgtcgtc gaaatcgttc
      841 atcgcgtcga gcgggcaggc gtcacaggcg tcgcccagac cgtcgtgatc gctgtccgtc
      901 tgcagcgcgt tggcgctctc cggacagttg tcggcgtagc ccggaagacc gtcgccgtcg
      961 gcgtcgttct ccgggtcggc ggggaagtag tcggggttgc agccgagatt ggggaaaccg
     1021 ggatcgccca gaccgtcgcc gtcgaggtcg gtgcagggat cgcaggcgtc ccccgccccg
     1081 tcgccatcgg tatccggctg gagcgagttc gaatggaccg ggcagttgtc catcgcgtcg
     1141 gcgacgccgt cgccgtcgtc gtccgcgtcg cacggggtgc cgaggccgtc ctcgtccagg
     1201 tcggcctggt ccgggttcgc caagctcgga cagttgtcgc agccgtcgcc gaagaagtcc
     1261 cgatccgcgt cggtctgcgg cgtgttggcg agcatcgggc agttgtcgtc gagatcgacg
     1321 accccgtcga ggtcggcgtc ggcggaaccc gccgagcacg agacggtggg aaagtgcggt
     1381 agccccgacg gctcgcgcgt cgcgggacct tcgccgcagc tgttgactcc cgacagcagg
     1441 tagtagaacc ccgcatgcgg cggaggcgtc gcaccgtccg tcgccgcgag gtcggcgttg
     1501 gccgcgttca agcagctctc gtcgtagctc cacgcgcccg cggcgaacgt gccgcggtag
     1561 acgtgggtca ccggtccctg ccaggtgcgt cgccacctga gggtggtgcc gctcgtcttg
     1621 tcgaggtcga gggtcgagcc gaagtcgccg ggaatgccgg ccagactcga tttgcccggg
     1681 gcacagtcgc acgggtcgcc gacgccgtca ccgtcgccgt ccgcttgacc ggcgttcgac
     1741 accttcgggc agttgtcgga cccgtcgagc agaccgtcgc cgtcgtcgtc ggcgtcgcac
     1801 gcgtcgccga tgccattgac gtcggtgtcg gtctggtcgg cgttcgcttc gagatcacag
     1861 ttgtcgggtc cgtcggccac gccgtcgccg tcgtcgtcgg cgtcgcacgc gtcgccgatg
     1921 ccgtccccgt cggtgtcggt ctggctcgga ttggacaacg ccggacagtt gtcgcaagcg
     1981 tcgccctcac cgtctccgtc gccgtcggtc tggctcacgt tgggcgtgct cgggcagttg
     2041 tcggcgtcgg cgcaatgtcc gtcgagatcg gcgtcgttgg ccgaatccgc cggacaggcg
     2101 tcgcaggcgt cccctgcgcc gtccccatcc gagtcggcct ggccgccgtt ggccaccgtc
     2161 gggcagttgt cagaggcggc gcagaccgag tcgaagtcgc cgtcgttcag cgggtcgccg
     2221 atgcacggat cgcaggcggt gcccaggccg tcgccatcct cgtccgcctg gctcgcattc
     2281 gccgtcgtcg agcagttatc gcagacgtcg ccgacgccgt cggtgtccgt gtccgactgc
     2341 gccgggttgg tcacggtcgg gcagttgtcg acgtcgccgc agtcgccgtc gaggtcagcg
     2401 tcgtccgccg ggtcgaacgg acagacgtcg cacgcgtcgc cgaagtcgtc gccatcggcg
     2461 tcggcttgac cgacgttcga gatcgccggg cagttgtcct ggctgtcgca caggccgtcc
     2521 gagtcggaat cggcgtcgtt gcactcgccg atgatgccga tctcgcgcag accgctgaag
     2581 tcctgggtgc gcgtgccggc ggcgaacatg ttcggcgcgc cgaaggtgct cgacgttccg
     2641 tcgttgtact cgttgaacgg cgtgatgaac gggctcggat cttcctcgag cttgaacacc
     2701 tcgtcgttgc cgatgccgct gaccggccag acgccctcgc cggccggccc gaataccggc
     2761 atcgccatct cgtcgaggat cgtcagctgc cagttgtcgt tcgacacggg gaagttcgac
     2821 gcggtgacgt agaggcccga ccctgcgtcg gcggcccgca cgttgatgtt ccagtcaccc
     2881 gacgcgggat cgtaggagac gttgtcggcc aagtcctcgg cgacggtgat gatcgtcccg
     2941 gcgcgcagat cggcccacag cggatcgttg g
//
LOCUS       DASPGN010000608         6141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_349921, whole genome shotgun sequence.
ACCESSION   DASPGN010000608 DASPGN010000000
VERSION     DASPGN010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349921"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VD788_17655"
     CDS             <1..221
                     /locus_tag="VD788_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78140.1"
                     /translation="SALHTDQNIAVKDIVVPPGVRLLDDPETIVAVVAELRIEEEPVP
                     EVAAGEAAAAAEGAAPAEAGEKEEGGES"
     gene            237..860
                     /gene="pth"
                     /locus_tag="VD788_17660"
     CDS             237..860
                     /gene="pth"
                     /locus_tag="VD788_17660"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXV78141.1"
                     /translation="MGSLAIVVGLGNPGERYRFTRHNLGFWVVDQLAARTGATLRADG
                     DLRRYAWTASVRWDDRPVLLAKPRTYMNRSGRAVAALLHGGGPAEKSLIVVYDDADLE
                     FGRIRIREEGGAGGHNGIRSLIEVLGHGNFARVRLGVRGEKRDGEELADYVLSEFAPN
                     EREPAAVLARLGAEAVRFLVEHGVAQAMNRWNGIRVPTSETECDDNG"
     gene            942..2975
                     /locus_tag="VD788_17665"
     CDS             942..2975
                     /locus_tag="VD788_17665"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001961.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HXV78142.1"
                     /translation="METWTNLIPLEGLLGLGFALTLYLTIARASAGTDRMREIAAEIH
                     SGAMAFLRREYSILVVFVAIVAALLGWFLDRQTAVAFLAGAACSVLAGFLGMAAATKA
                     NVRTAAAAKEGGQGPALLMAFHGGSVMGVSVASLGLVGVGVFFYLYGDPVSAKYINGF
                     AMGASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPGVIADNVGDNVGDV
                     AGMGADIFESYVGSIVATIAIGATLPISARVMQHAADPEAVRPVLMALPLLLASIGLI
                     ASLLGILSMRVLKQFNPASALRNSTFIAAAGFLAGAYLVIRAYDVTSGVFWAVLCGTL
                     VGILIGLITEYYTAGRPVRRIAEASKTGPATNIISGLAVGLESTALPVLCICLAIWIG
                     NEFAGLYGIGVAAVGMLATVGITMSVDAYGPVADNAGGISEMAHLGPQVRKITDGLDA
                     LGNTTAAIGKGFAIGSAALTALALFTAYRQAVPSEHGGLEIVLTDPVVVIGLLVGGIL
                     PFLIGAMTMTSVGRAAEQMVAEIRRQFREIPGLLEGRTGAKPDAARCVDISTRAALKE
                     MVAPGLTAVAAPVVVGFGLGPAALGGMLAGATVSGVLLALFMANAGGAWDNAKKAIEK
                     GDVAGEEKGSQAHKAAVVGDTVGDPFKDTSGPSMNILIKLMSIVSLVIAPLLG"
     gene            3130..3624
                     /gene="rpsF"
                     /locus_tag="VD788_17670"
     CDS             3130..3624
                     /gene="rpsF"
                     /locus_tag="VD788_17670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXV78143.1"
                     /translation="MHTYETLFITPPTLSDAEEQSTVDTMARVVADGGGSMVANERMG
                     RRRLAYPISKHEDGVYVRFLYDAEPAVPKELERRIRLSDQILRSLTVRLEDDWAVAAK
                     EEAVHEAERRARAEEERLAAEAAAAASGVAPTAASTEAGPVSSAAGRFDDDDDDDRST
                     RDDD"
     gene            3680..3928
                     /gene="rpsR"
                     /locus_tag="VD788_17675"
     CDS             3680..3928
                     /gene="rpsR"
                     /locus_tag="VD788_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXV78144.1"
                     /translation="MARQGYGGRRRFLFRRRKYCKFCESKVSWIDHKDLKTIQSYIPE
                     RSKILPRRISGTCAKHQRLLMRAIKRARMIAFVPFTSD"
     gene            3938..4453
                     /gene="rplI"
                     /locus_tag="VD788_17680"
     CDS             3938..4453
                     /gene="rplI"
                     /locus_tag="VD788_17680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HXV78145.1"
                     /translation="MKIILREHVDRLGQRGEIVNVAAGFARNYLLPKGLAYLATPGKI
                     KQLEQQRRVWHARDLKEQHEAEQVAARLAQLEIRIEHKAGEAGTLYGAVTAAEIASLL
                     ERNGVAIDRRKLELGGPIKSLGQHEIRVRLHPKVMGTFQVLVLPEDGKMPQTESVVEE
                     SEPAADGEDSE"
     gene            4457..4945
                     /locus_tag="VD788_17685"
     CDS             4457..4945
                     /locus_tag="VD788_17685"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HXV78146.1"
                     /translation="MGDRGSRDVAGQEWNARDLRRLALLVGAGWAVPGLGHMLLGRAA
                     RGLLFALLIWGAFGLGLAHDGRLALRDARQPVLSSLQVLANVGVGPADLLARIVVYGA
                     PVYTFRGIPDWRSEDRVERIFRERDRSAVSIYGTAYLWTAGLMNLLLLFDVWDIGLRR
                     KT"
     gene            4948..5121
                     /locus_tag="VD788_17690"
     CDS             4948..5121
                     /locus_tag="VD788_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78147.1"
                     /translation="MRSHFLHMLVYSTVVATFFATMFRDTSRERIRLGAILWLAMVGG
                     GLLLAWIMFPFPA"
     gene            5195..>6141
                     /locus_tag="VD788_17695"
     CDS             5195..>6141
                     /locus_tag="VD788_17695"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF002898.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXV78148.1"
                     /translation="MIKRFAVVPGDGIGIDVTREAVKVLDAAAAAEGFDIETVSYDYG
                     AERFLETGETLPDTALEEFKRFDAVLMGALGDPRVPDMRHARDILLGTRFRLDLYVNL
                     RPIRCLDDRLCPLKRFNASDIDFVVFRENTEGLYVGMGGIFKKGTPDEIAIQEDVNTR
                     KGVERIIRAAFDYARSHSRPNVTMSDKSNALRYGHDLWQRVFREVGAEYADIEQRHMY
                     VDALTMQMLKDPTQFEVIVTCNMFGDILTDLGAQLQGGMGLAASANLNPGRTGLFEPV
                     HGSAPKYAGQDIANPMGAILSAALLLETLDRIEEAGRIE"
BASE COUNT          961 a         1963 c         2240 g          977 t
ORIGIN      
        1 tgtcggcgct ccacaccgat cagaacatcg cagtcaaaga catcgtggtt ccgcctgggg
       61 tacggctgct cgacgacccc gagacgatcg tcgcggtcgt cgccgagctg agaatcgagg
      121 aggagccggt tcccgaggtg gcagccggcg aagcggcagc ggcagcggaa ggggcggcgc
      181 cggccgaggc aggcgagaag gaggaaggcg gggagtcctg atccgcctga cgtgccgtgg
      241 ggtcgctggc catcgtcgtc ggactgggca accccggcga gcgctatcgc ttcacccgcc
      301 acaacctcgg tttctgggtg gtcgatcagc tggccgcgcg cacgggagcg acgttgcggg
      361 cggacgggga tctgcgtcga tacgcgtgga cggcgtcggt tcggtgggac gaccgcccgg
      421 tgctgctggc caagccacgg acgtacatga accgctcggg acgcgcggtc gcggcgttgc
      481 tccacggcgg ggggccggcg gagaagtcgc tgatcgtcgt ctacgacgac gccgacctcg
      541 agttcggtcg aatccggatc cgtgaggagg gtggggccgg tggtcacaac gggattcggt
      601 cgctgatcga ggtgctgggg cacgggaatt ttgcgcgcgt gcggctgggg gttcgcggcg
      661 agaaacggga cggcgaggag ctggccgact acgtcctgtc cgagttcgcc ccgaacgagc
      721 gcgagcctgc cgcggtgttg gctcggctgg gggcggaggc ggtccgcttc ctggtcgagc
      781 acggcgtcgc gcaggcgatg aaccgctgga acgggattcg ggttccgacg agcgagacgg
      841 agtgtgacga caacggctga gatcgtccgg cgtcggcggg cgatcgactg agcgtgaagg
      901 cgacccgacg gcgggcgccc cggtgaagga gcgacaacga aatggagacg tggaccaatc
      961 tcatcccgct cgagggcctg ctggggctcg gcttcgcgct gacgctgtac ctgacgatcg
     1021 cccgtgcgag cgccgggacc gatcggatgc gggagatcgc cgccgagatc cacagcgggg
     1081 cgatggcgtt tctccgccgc gagtattcga tcctcgtcgt gttcgtcgcg atcgtcgcgg
     1141 cgctgctcgg ctggttcctt gaccggcaaa cggcggtcgc gttcctggca ggcgcggcgt
     1201 gctcggttct cgcgggcttt ctcggcatgg cggcggcgac gaaggccaac gtgcggaccg
     1261 ccgcggcggc caaggaaggt gggcaggggc ccgcgctgtt gatggcgttc cacggtggct
     1321 cggtcatggg agtctcggtc gccagcctcg gtctggtcgg tgtcggcgtc ttcttctacc
     1381 tctacggcga ccccgtcagc gccaagtaca tcaacggctt cgcgatgggc gcctcgtcga
     1441 tcgcgttgtt cgcgcgggtc gggggcggga tctacaccaa ggcggccgac gtcggcgccg
     1501 acttggtcgg caaggtcgag gccgggattc ccgaggacga tccgcgcaac cccggcgtga
     1561 tcgcggacaa cgtcggggac aacgtcggcg acgtcgccgg catgggcgcg gacatcttcg
     1621 agtcttacgt cggatcgatc gtggccacga tcgcgatcgg agcgaccctg ccgatcagcg
     1681 cgagggtgat gcagcacgcg gccgacccgg aggcggtgcg acccgtgttg atggcgttgc
     1741 cgctgctgct ggccagcatc gggctgatcg cctcgctcct cggcatcctg tcgatgcggg
     1801 tgctcaagca gttcaacccg gcgtcggcgc tgcgcaactc gacgttcatc gccgccgccg
     1861 gcttcctcgc cggcgcctat ctggtgattc gggcctatga cgtgacgagc ggtgtcttct
     1921 gggcggtgct gtgcggaacg ctcgtcggga ttctcatcgg cttgatcacc gagtactaca
     1981 ccgcgggcag gccggtccgg cgcatcgccg aggcgagcaa gaccggtccg gccacgaaca
     2041 tcatcagcgg cctggccgtc ggtctcgaat cgaccgcgtt gccggtcctg tgcatctgcc
     2101 tcgcgatctg gatcggcaac gagttcgccg gcctctacgg tatcggcgtc gccgcggtcg
     2161 ggatgctggc gaccgtgggc atcacgatgt ccgtcgacgc ctacgggccg gtggcggaca
     2221 acgccggcgg catttccgag atggcgcacc tcggccccca ggtgcgcaag atcaccgatg
     2281 gtctcgacgc gctgggcaat acgacggcgg cgatcggcaa gggcttcgcc atcggctcgg
     2341 cggcgctgac ggcgctggcg ctgttcaccg cctaccgcca ggcggtgccg agcgagcacg
     2401 gcgggctcga gatcgtcctc accgacccgg tcgtcgtgat cgggctgctc gtcggcggaa
     2461 tcctgccgtt cctcatcggc gcgatgacga tgacgtccgt cggtcgcgcg gcggagcaga
     2521 tggtggcgga gatccgtcgc cagttccgcg agatcccggg gctgctcgag ggccggaccg
     2581 gggccaagcc cgacgccgcc cgctgtgtcg acatctcgac ccgcgcggcg ctcaaggaga
     2641 tggtggcgcc cgggctgacc gcggtcgccg cgccggtggt ggtcggcttc ggtctcggcc
     2701 cggccgcgct cggtggcatg ctcgccggcg caacggtcag cggcgtgctg ttggcgctgt
     2761 tcatggccaa cgccggcggc gcgtgggaca acgccaagaa agcgatcgag aagggcgacg
     2821 ttgccgggga ggagaagggg tcccaggcgc acaaggcggc cgtcgtcggc gataccgtcg
     2881 gggacccgtt caaggacacc tccgggccgt cgatgaacat cctgatcaag ctgatgtcga
     2941 tcgtgagcct ggtgatcgcg ccgctgctgg gttgagacgc cccggcggcc gcggctggcg
     3001 gtcgcccggc gacggtggta tcgttccggc cgcggcaggc ggcccgcgcg gaccgccccc
     3061 gggaccggtt cgatcccgca ctccttgcca ccggcgcgac cggtggccga aggccatgag
     3121 gaggtgctcg tgcacactta cgagactctg ttcatcactc ccccgacgct ctccgacgcg
     3181 gaagagcaga gcaccgtcga cacgatggcg cgtgtcgtcg ccgatggcgg cggtagcatg
     3241 gtggccaacg aacggatggg tcggcgccgc ttggcctatc cgatcagcaa gcacgaggat
     3301 ggcgtctacg tccgattcct gtacgacgcg gagcctgcgg tgcccaaaga gctcgagcgc
     3361 cgcatccgat tgtccgacca gatcctgcgc agcctgaccg tccgtctcga ggacgactgg
     3421 gcggtggcgg ccaaggaaga ggcggtgcac gaggccgagc gccgcgcgcg cgccgaggaa
     3481 gagcggctgg ccgccgaagc cgcggcggcc gcgtccggcg tcgcaccgac ggccgcgtcg
     3541 accgaagcgg gtccggtttc gtcggccgcc ggccggttcg atgacgatga cgacgacgac
     3601 cgctcgacga gagacgacga ctgagcgccg gcgcggtcga gcggaagcgc ggcgccagac
     3661 cgcgatggga gagggtccga tggcacgaca ggggtacggg ggacggaggc gcttcctctt
     3721 tcgccgccgg aagtattgca agttctgtga atccaaggtg agctggatcg accacaagga
     3781 cctcaagacg atccagagct acattcccga gcgctcgaag atcctgccgc gaaggatctc
     3841 gggcacctgc gccaagcacc agcgcttgct gatgcgggcg atcaagcgcg cacggatgat
     3901 cgccttcgtc ccgttcacgt ccgactgagg tgtcgcggtg aagatcattc tgcgagaaca
     3961 cgtcgaccgg ctcggccaac gcggcgagat cgtcaacgtc gccgccggct tcgcccgcaa
     4021 ctacctgttg ccgaaggggc tggcctacct ggcgacgccg ggcaagatca aacagctcga
     4081 gcagcagcga cgcgtgtggc acgcgcgaga cctgaaggag cagcacgagg cggagcaggt
     4141 tgccgcccgg ctggcgcagc tcgagatccg catcgagcac aaggcgggtg aggccggcac
     4201 gctctacggt gcggtgaccg cagcggagat cgcgtcgctg ctcgagagaa acggcgtggc
     4261 gatcgatcgg cgcaagctgg aactcggcgg cccgatcaag tcgctcggcc agcacgagat
     4321 ccgcgtgcgg ttgcacccca aggtgatggg tacgttccag gtgctcgtgc tgcccgaaga
     4381 cggcaagatg ccgcagaccg agagtgtggt cgaggagtcc gaacccgccg ccgacggcga
     4441 ggacagcgaa taacggatgg gcgatcgagg ctcgcgcgac gtggccggtc aggagtggaa
     4501 cgctcgtgat ctgcgccgac tggcactcct ggtcggcgcc ggttgggccg ttccggggct
     4561 cggtcacatg ctgctgggac gcgcggcacg cggcttgctg ttcgccttgc tgatctgggg
     4621 cgcgttcggt ctcgggctgg cgcacgatgg gcgactggcg ctgcgcgacg cgcggcagcc
     4681 ggtgctgtcg tcgctgcagg tgctggccaa cgtcggcgtc ggtccggccg atctgctggc
     4741 tcggatcgtg gtttacggcg cgccggtcta caccttccgc ggcattcccg actggcgctc
     4801 ggaggaccga gtcgaacgga tcttccgcga gcgcgaccgc tcggcggtgt cgatctacgg
     4861 aaccgcctac ctatggaccg ccggcctgat gaacctgttg ctgctgttcg acgtgtggga
     4921 catcggcctg cgccggaaaa cctagtcgtg cgcagccatt tcctccacat gctcgtctat
     4981 tcgaccgtcg tcgcgacctt tttcgcgacg atgttccgcg atacgtcgcg cgagcggatc
     5041 cggctgggag cgatcctgtg gctggcgatg gtcggcggag gcctgctctt ggcctggatc
     5101 atgttcccgt tcccggcttg agcctgtgcg cgtcgaaacg cccggacgga tcgggccaac
     5161 ggccacatcg cgccggtcgt gggagacgga gacgatgatc aagcgattcg cggtggttcc
     5221 cggggatggc atcggcatcg atgtcacccg cgaggcggtc aaggtgctcg acgcggcggc
     5281 ggcggccgaa ggcttcgaca tcgagaccgt ctcctacgac tacggagcgg agcgattcct
     5341 cgagaccggc gagaccctgc cggacaccgc gctcgaggaa ttcaagcggt tcgacgccgt
     5401 cttgatgggc gcgctcggcg acccgcgggt cccggacatg cgccacgccc gcgacatcct
     5461 gctcgggacc cggtttcgac tcgatctcta cgtcaaccta cggccgatcc gctgcttgga
     5521 cgaccggctc tgtccgctga agagattcaa tgcgtcggac atcgacttcg tcgtgttccg
     5581 cgagaatacc gaggggctgt acgtcggcat gggcgggatc ttcaagaagg gaacgccgga
     5641 cgagatcgcc atccaggaag acgtcaatac gcgcaaggga gtcgagcgca tcatccgcgc
     5701 cgcgttcgat tacgcccgct cgcattcgcg gccgaacgtg acgatgtcgg acaagtccaa
     5761 cgcgctgcgc tacgggcacg atctgtggca gcgggtgttt cgcgaggtcg gcgcggagta
     5821 cgccgacatc gagcagcgcc acatgtacgt cgacgcgctg acgatgcaga tgctgaagga
     5881 cccgactcag ttcgaggtga tcgtcacctg caacatgttc ggcgacatcc tgaccgatct
     5941 gggcgcgcaa ctgcagggag ggatggggct ggcggcttcg gcgaacctca acccgggccg
     6001 caccggcctg ttcgagccgg tgcacgggtc cgcccccaag tacgccgggc aggacatcgc
     6061 caatccgatg ggcgcaatcc tgagcgccgc gctcctgctg gaaaccctcg accgcatcga
     6121 agaggcgggc cgcatcgaga g
//
LOCUS       DASPGN010000609         4320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1519045, whole genome shotgun sequence.
ACCESSION   DASPGN010000609 DASPGN010000000
VERSION     DASPGN010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519045"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VD788_17700"
     CDS             <1..206
                     /locus_tag="VD788_17700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXV78149.1"
                     /translation="GGGLFTIYAHLSAIEVDEGQGVRRGTTLGRSGATGRVTGPHLHW
                     GAKIGDRPFDPTALLDPRLFEPR"
     gene            complement(214..2577)
                     /locus_tag="VD788_17705"
     CDS             complement(214..2577)
                     /locus_tag="VD788_17705"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78150.1"
                     /translation="MARGGSQAVERLYRESERSFERWEVVKDLIDEMIDISLNYRQSG
                     HPGGSRSKVHAMVALLLSGAMRWDVARPWCPFADRFVLSAGHTVPLVYATLAVLNEAM
                     RVRHRRTRDDAFAFPAGGKFALTWEDLLGLRRNRGLAGHAEMEGKTLFLKWNTGPSGH
                     GMPAAAGEALALKRAGAGEVKVFTVEGEGGLTPGASHETRNTAWGLGLDNLVFIIDWN
                     DFGIDDNAISSVVHGTPQSWFEAYGWKTLGTDKGSEWPTVTRTLLEVVLGDNPRNVPC
                     AAWFKTRKGRGYLVYDNASHGVPHKMNSEKFWELRRRFMEKYGVSYEGVDQGPPNGAA
                     AVRAQAEANLRIAMRVIQDDSALADYVSDRLVESARSVPRELPSRRWGKAGAKLFADE
                     RLFDFRRYPGEMWAKPGDQQPNRAALAAWGAWVNSFARKEYGRPLFLVCSADLAASTN
                     IAGFASGFGGVDGWGWYNRDDKPEGVLLPQQITEFTNAGLSVGAASVNLAADPLREFD
                     GFWTACSTYGSFSYLKYGPMRLFSQLAQDCELRVGKVLWIAGHSGPETAEDSRTHFGI
                     FAPGVTQLFPDGQVIDLHPWEYNEVPVVLGAALATDVPIVALHLTRPKVTIPDRAGLG
                     IASHFEAARGAYLIRDYRAGQPRMGTVFVQGTMTTHNLVGLLPELDRRGVNVKIVAAI
                     SPQLFAMQAESYRERVAPLADRWDAMMISNRSKRVSRDWIENSIVADYSLTSDRDNRW
                     RTGGSVEEVIAEARLAPADILDGIERFARERGERIRRLRAALAAIEG"
     gene            complement(2598..3800)
                     /locus_tag="VD788_17710"
     CDS             complement(2598..3800)
                     /locus_tag="VD788_17710"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXV78151.1"
                     /translation="MPELSRRARHLGTENAFVVLAEVERLIAEGQRIVSFCIGQPDFP
                     TPDNVRLAGIRAITDGKTGYTASPGIPALRQAVARFLSRTRGIDVDPADVVCGCGGKP
                     FIGYAVLSVTDYGEGHEVIYPNPGFPIYSSQIAANGAVPVPLDLRESLSFNFDPDELA
                     RRVSDKTRLLILCSPGNPTGSVLGRRELEQIAEIVGPFPDLWIYSDEVYSGLVYDGEF
                     QSIAAVPGMQERTIIVDSASKTYSMTGWRIGYAANRALAPIFTRWVTNTDSCPPHPNQ
                     FAVVEALDGPQDAAREMRDVFLRRRDMIVAGLNRIRGWSCLTPGGAFYVWPNVTEACR
                     MVGVDNSEELRKRLLHEAGVAVLADIHFGRRVTGDGEHVRFSYASSFDAIEEGLARIA
                     RFMADNKR"
     gene            complement(3944..>4320)
                     /locus_tag="VD788_17715"
     CDS             complement(3944..>4320)
                     /locus_tag="VD788_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78152.1"
                     /translation="GDLIVRVADRPIGEVADLRSALDDHAGETFRIEVVRDRERIELD
                     VTLPEADEDEVTGPRAALAPRALPAPVTAPPLRPRPVLAAAPRVVVPPLPPRPALAAP
                     GAPAVAVPLPRPAPVPRIDRTL"
BASE COUNT          683 a         1491 c         1490 g          656 t
ORIGIN      
        1 acggcggcgg gctgttcacg atctacgccc atctgtccgc gatcgaggtc gatgagggac
       61 agggcgtccg ccgcggaacg acgctcggcc gttccggggc gaccgggcgg gtgacgggac
      121 cccatctgca ttggggggcc aagatcggcg atcgcccgtt cgacccgacg gcgctgctcg
      181 atccgcgtct gttcgagcct cgttagctct gagctagccc tcgatcgcgg cgagcgcggc
      241 gcgcagccgg cgaatccgct cgccgcgctc gcgcgcgaac cgctcgatgc cgtcgaggat
      301 gtccgctggc gccaaccgcg cctcggcgat cacctcctcg acgctgccgc cggtgcgcca
      361 gcggttgtcc cgatcggagg tcagcgagta gtcggcaacg atcgaattct cgatccagtc
      421 gcggctgacg cgcttcgaac ggttgctgat catcatcgcg tcccagcgat cggccagcgg
      481 cgcgacccgt tcccgatagc tctcggcctg catcgcgaac aactgcgggc tgatcgccgc
      541 cacgatcttg acgttgaccc cgcgacggtc cagctcgggc agcagaccga cgagattgtg
      601 tgtcgtcatc gtgccttgga cgaaaaccgt ccccatccgt ggctgccccg cgcggtagtc
      661 gcggatcagg taggcaccgc gcgccgcttc gaagtgcgac gcgatgccca gccccgcgcg
      721 gtcggggatc gtgaccttgg gtcgggtcag gtgcagcgcg acgatcggca cgtcggtcgc
      781 gagcgcggcg ccgagcacga ccggcacctc gttgtactcc cacggatgaa ggtcgatcac
      841 ctgcccgtcg gggaacagtt gggtcacgcc gggggcgaag atgccgaagt gcgtgcgcga
      901 gtcttccgcc gtctcgggtc ccgagtgacc ggcgatccac agcactttgc cgacccgcag
      961 ctcgcagtcc tgcgccaact ggctgaacag ccgcatcggc ccgtacttga gataggagaa
     1021 cgagccgtag gtcgaacagg cggtccagaa gccgtcgaac tcacgcagcg ggtcggcggc
     1081 caggttgacc gaggccgcgc cgaccgacag cccggcgttg gtgaactcgg tgatctgttg
     1141 cggcagcagg acgccctcgg gtttgtcgtc gcggttgtac cagccccagc cgtcgacccc
     1201 gccgaagccg ctggcgaaac cggcgatgtt ggtcgacgcg gcgagatcgg ccgagcagac
     1261 gaggaacaac ggccggccgt actccttgcg cgcgaacgag ttgacccacg caccccaggc
     1321 ggcgagcgcc gcgcggttcg gttgctggtc gcccggcttg gcccacatct cgcccgggta
     1381 gcggcggaag tcgaacaacc gctcgtcggc gaacagcttc gctccggctt taccccagcg
     1441 ccgcgacggc agctcgcggg ggaccgaccg cgccgactcg acgagccggt cgctgacgta
     1501 gtccgccagc gccgaatcgt cctgtatcac gcgcatcgcg atccgcaggt tcgcctcggc
     1561 ctgcgcgcgg accgcggccg cgccgttcgg cggcccctgg tcgacgcctt cgtacgacac
     1621 gccgtatttt tccatgaagc ggcggcgcag ctcccagaac ttctcgctgt tcatcttgtg
     1681 cggcacgccg tgcgatgcgt tgtcgtagac cagatagccg cgacctttgc gcgtcttgaa
     1741 ccaggcggcg cagggtacgt tccgtgggtt gtcaccgagc acgacctcga gcagggtgcg
     1801 agtgaccgtc ggccattcgg agcccttgtc ggtgcccagc gtcttccaac cataggcctc
     1861 gaaccaggac tgcggcgtac cgtgaacgac cgacgagatc gcgttgtcgt cgatgccgaa
     1921 gtcgttccaa tcgatgatga acaccaggtt gtcgaggccg agtccccagg cggtgttgcg
     1981 cgtctcgtgg ctcgcgccgg gcgtcaaccc accctcgcct tcgacggtga acaccttgac
     2041 ctcgccggcg cccgcgcgct tgagcgccag cgcctcgccc gccgccgccg gcatgccatg
     2101 tcccgacggc ccggtgttcc acttgaggaa cagcgtcttg ccctccatct cggcgtggcc
     2161 ggcgagcccc ctgttgcggc gcaagccgag caggtcttcc caggtcaagg cgaacttccc
     2221 gccggcggga aaggcgaacg cgtcgtcgcg ggtccgccga tgccgcacgc gcatcgcctc
     2281 gttcagcacg gccagcgtcg cgtagaccag cggcacggtg tggcccgccg acagcacgaa
     2341 ccggtcggcg aacgggcacc atggtcgcgc gacgtcccag cgcatcgcac ccgacaacaa
     2401 caacgcgacc atcgcgtgca ccttcgagcg cgaaccaccg gggtggccgc tctggcggta
     2461 gttcaacgag atgtcgatca tctcgtcgat caggtccttg acgacctccc accgctcgaa
     2521 cgagcgttcc gactctcgat aaagacgctc aaccgcctgg ctaccgccgc gagccacggg
     2581 atacctccgc gaaagcgcta tcgcttgttg tccgccatga accgcgcgat gcgcgccaga
     2641 ccctcttcga tcgcgtcgaa cgacgaggcg taggagaagc ggacgtgctc gccgtcgccg
     2701 gtgacgcgcc ggccgaagtg gatgtcggcc agcaccgcca cgcccgcttc gtgcagcagc
     2761 cgtttgcgca gctcctcgct gttgtcgacg ccgaccatgc ggcaggcctc cgtcacgttg
     2821 ggccagacgt agaacgcgcc gccgggggtg agacaggacc agccccggat gcggttcaac
     2881 ccggcgacga tcatgtcgcg tcgtctgaga aacacgtcgc gcatctcgcg cgccgcgtcc
     2941 tgcgggccgt ccagcgcttc gaccaccgcg aactgattgg gatgcggcgg gcaagagtcg
     3001 gtgttggtga cccaacgggt gaagatcggc gccagcgctc ggttcgccgc gtagccgatg
     3061 cgccagccgg tcatcgagta ggtcttcgac gccgagtcga cgatgatcgt gcgctcctgc
     3121 atgccgggaa cggcggcgat gctctggaac tcgccgtcgt agaccaatcc ggagtagacc
     3181 tcgtccgagt agatccacag atcggggaac ggtccgacga tctcagcgat ctgctccagc
     3241 tcgcgtcggc cgagcaccga accggtcgga tttcccgggc tgcacaggat cagcaatcgg
     3301 gtcttgtcgc tcacccggcg ggccagctcg tcggggtcga agttgaacga cagagactcg
     3361 cgcaggtcga gcggcaccgg gacggcgccg ttggccgcga tctgcgagga gtagatcggg
     3421 aagccggggt tggggtagat cacctcgtgc ccctcgccgt aatcggtgac cgacagcacg
     3481 gcgtagccga tgaacggctt gccaccgcag ccgcagacca cgtccgccgg atcgacgtcg
     3541 ataccgcgcg tgcggctcag gaaacgagcg accgcttggc gcaacgccgg gatcccgggc
     3601 gaggcggtgt agccggtctt gccgtcggtg atcgcgcgga tcccggccag tcgcacgttg
     3661 tccggcgtgg gaaagtcggg ttgtccgatg cagaacgaga cgatccgttg gccttcggcg
     3721 atcagacgtt cgacctcggc gaggacgacg aacgcgttct cggtgccgag gtggcgcgcg
     3781 cggcgggaaa gctcgggcat ggccgcattc tcctggggat cccgttgggg agagtgcgga
     3841 attgtagccg tccgggtggg gcgccatcaa gggcggaacc gcgcacaaac aggacacggc
     3901 ccggtctgag ccgacgagcg gcagcgggga acgaccgcgc ggactacagc gtccggtcga
     3961 tccgcggcac gggagccggc cggggcaacg gaaccgcgac ggccggagcc cccggcgcgg
     4021 cgagcgccgg tctgggcggg agcggcggca cgaccacgcg cggggcggcg gcgagcaccg
     4081 gtctgggccg aagcggcggc gcggtgacgg gcgccggcag cgcgcgcggc gcgagcgccg
     4141 cgcgcggacc ggtcacctcg tcctcgtcgg cctcgggcag cgtcacgtcg agctcgatcc
     4201 tctctcggtc gcgcaccacc tcgatgcgga aggtctcgcc cgcgtgatcg tccagtgccg
     4261 atcgcaggtc ggcgacctcg ccgatcggcc ggtcggcgac gcggacgatc aggtcgccga
//
LOCUS       DASPGN010000610         6066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1637439, whole genome shotgun sequence.
ACCESSION   DASPGN010000610 DASPGN010000000
VERSION     DASPGN010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637439"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1478)
                     /locus_tag="VD788_17720"
     CDS             complement(9..1478)
                     /locus_tag="VD788_17720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXV78153.1"
                     /translation="MSTEGARTRPRKLGSYEIVEEIGSGGMGVVYLARQPALERAVVL
                     KRMRRDLVDDDGLVERFEREARAAAAVHHQNVVAVYDCFTMRGDHYIAEEYVDGPDLR
                     TILEHVDRLHPRIAAIIGLQVIRGLEEIHARGIVHRDLKPANVLVGSGGETKIADFGI
                     ALDGKAYGLTRPGTLMGSVPYLSPEQLLGERVDDRSDLFVFGILLYEMVVGEPPYDES
                     REDSTDTLLERMQNGRYLPPRKRVPTVPRHLARLIKACLQAKPAKRIATATQVRRQLE
                     WHMGRISPADCRYRIAAWLWESGIIARDGDETEARPLAGDAGAGWGDWFRRPIVRWAG
                     PFVLAGLLIGLGSAASVLGLFESDWPELPRQRSGAADLPPVEPTREAAVPVVPPGLSS
                     RGPAADGPRPDSTSESEPAPVALEPAAVRLVASPWARVSIDGQQPFLTPRAAAVPLEP
                     GRHLIVFEHPRFGRAEYEIDLAPGDERQLVHRYEEGQPR"
     gene            complement(1499..1900)
                     /locus_tag="VD788_17725"
     CDS             complement(1499..1900)
                     /locus_tag="VD788_17725"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXV78154.1"
                     /translation="MQDGLTRKLKTRPARTDDSVLGRSFKVVVLSGPRAGAAYELDRR
                     RVTLGRGPGVDLAFDDAALSRQHAAFDFEDGGYRIRDLGSTNGLRVNGNRVQLCDLDN
                     GDHIEMGTLVFQLVVEERETPPEVWDLSTER"
     gene            complement(1996..2505)
                     /locus_tag="VD788_17730"
     CDS             complement(1996..2505)
                     /locus_tag="VD788_17730"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HXV78155.1"
                     /translation="MRTFIATAVLLTALSLDGSPLRADAAGDEEASRRLASLGWLAGH
                     WRGPADEGEWEAAYTGADGGTILSANKQIVDGRVASIEFERFVVREGQVVMMPSPGGR
                     PSEVHFTLTELDAEARRAVFTNPEHDFPQILTYHRVAPDRLRIDVEALRDGRRVGFHI
                     ELRPVAGKP"
     gene            2590..3894
                     /locus_tag="VD788_17735"
     CDS             2590..3894
                     /locus_tag="VD788_17735"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV78156.1"
                     /translation="MGPDRADRFGIATLALLALGLVSTATADDWPQWRGPERDGRSAE
                     TGLLDNWPAGGPPLEWRASGLGSGFSSVAVAGERIYTLGDSGDAQYAVALDRKDGKQL
                     WKTRVGPAWDDEYLGPRSTPTVDGKRIFVLSTEGDLFAIDAESGVTVWQRNLVKDFGG
                     AMMKAMGQYEWKYSESPLVDGDRVVVTPGGKEAMMVALAKATGEEIWRVAIPSLGEKG
                     TDGAGYSSPVVSEAGGVRQYVQFVGRGVIGVEAKTGRFLWGYNKVANDVANIPTPLVD
                     GDRVFASSGYGTGSALIELSRGDGGFSAREVYFLGPDTMQNHHGGLILHDGHVYAGSG
                     HNKGLPVSVELATGKVAWGPERNDGQASAAIAYADGRLYFRYQNGLMVLVEATPEGYR
                     EHGSFTIPDVKRPSWPHPVIAGGKLYLREQDHLYCYDVRARG"
     gene            3982..5283
                     /locus_tag="VD788_17740"
     CDS             3982..5283
                     /locus_tag="VD788_17740"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXV78157.1"
                     /translation="MTGRAASGRAVFIVDARRTPFLRSGSEFRELCAYDLGRMAIAGL
                     LHKSGVDRTTIDRVVLGTVVADPDTSNVARESALAGGVPASCPANTVTAACISANIAF
                     EQAAHAIATGEADVAIAGGTETLSDVPIRVSRAVRKRLIAARKVRGIGGWLALLRGLR
                     PRDLLPVVPAIADFSTGLTMGQNAERLAKRIGISRHDQDEYAMLSHHRAAEAAGRGWL
                     DSQIVPARLPREFRPIDRDNGIRGDTSMEKLAELKPAFDRRFGTITAGNSSYLTDGAS
                     AVLLASEDGLAETGLPPLARVVSQAVSAMDPLEELLLGPSFAIPAALDRAGLTLGDLD
                     VIELHEAFAAQVLAVIRLLADARFARDSLSRDRAVGRIDIERTNRWGGSLSVGHPFGA
                     TGGRLVANCCRRLIHEDGRFGLVAACASGALGYAMVVERVG"
     gene            5283..>6066
                     /locus_tag="VD788_17745"
     CDS             5283..>6066
                     /locus_tag="VD788_17745"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXV78158.1"
                     /translation="MAPGAVRLDIRADGVAIVTFDTPESKVNILSRELFDEIATVLDR
                     IESERAVRAAVLSSGKPGSFIAGANLEQLKSIDREEEGRRFSSDGQRLLDRIAESPKP
                     FVAAIHGPALGGGLEVALACHYIVASDSPSTVLALPEVMLGLLPGGGGTQRLPRRVGL
                     VEALPLMLTGRRLRARRAYRIGLVDAVTSPGGIAETAARAASMLADGKLSRRRVSRPL
                     ANRLLEIAPFRALVFRRARAQVRRQTRGNYPAPPFIVDCAATG"
BASE COUNT          859 a         2125 c         2180 g          902 t
ORIGIN      
        1 ggttcgtttc atcgcggttg cccctcttcg tagcggtgca cgagctgccg ctcgtcgccc
       61 ggggccaggt cgatctcgta ctcggcgcgc ccgaagcgcg ggtgctcgaa gacgatcagg
      121 tgcctgcccg gttcgagcgg aacggcggcc gcccgcgggg tgaggaacgg ctgctgcccg
      181 tcgatcgaca cccgagccca cggcgaggcg accagccgca ccgcggccgg ctcgagcgcg
      241 accggcgccg gctcggactc gctcgtgctg tcgggccggg gcccgtccgc cgcgggcccg
      301 cgcgacgaga gaccgggcgg cacgacgggc acggcggcct cgcgcgtggg ctcgaccgga
      361 gggaggtcgg ccgcacccga tctctggcgc ggcagctccg gccagtcgga ttcgaacaga
      421 ccgagcacgc tcgcggccga gccgagcccg atcaacagac cggcgagcac gaacggaccg
      481 gcccagcgta cgatcggccg gcggaaccag tcgccccagc cggcgccggc gtcgcccgcg
      541 agcggacggg cctcggtctc gtcgccgtct cgcgcgatga tgcccgactc ccacagccag
      601 gccgcgatgc ggtagcggca gtcggcgggc gagatgcggc ccatgtgcca ctcgagctgg
      661 cgccgcacct gggtcgcggt cgcgatgcgc ttggccggtt tggcctggag gcacgccttg
      721 atcagtcgtg ccagatggcg cggcacggtc ggcacgcgct tgcgcggcgg gaggtaacgg
      781 ccgttctgca tccgctcgag cagcgtgtcg gtcgagtctt cgcgcgactc gtcgtacggt
      841 ggctcgccga cgaccatctc gtacagcagg atgccgaaga cgaacaggtc gctccggtcg
      901 tcgacgcgct cgccgagcag ttgctcgggc gacaggtacg gcaccgaccc catcagcgtg
      961 cccggccgcg tcagcccgta cgccttgccg tcgagcgcga tgccgaagtc ggcgatcttg
     1021 gtttcgccgc cgctgcccac cagcacgttg gccggcttga ggtcacgatg cacgatgccg
     1081 cgggcgtgga tctcttccaa cccgcggatg acctgcagac cgatgatcgc ggcgatgcgc
     1141 ggatggagcc ggtcgacgtg ctcgaggatc gtccgcaggt cgggaccgtc gacgtactcc
     1201 tcggcgatgt agtgatcgcc gcgcatcgtg aaacagtcgt agaccgcgac gacgttctga
     1261 tggtgcacgg cggcggcggc gcgcgcctcg cgctcgaatc gttcgaccaa cccgtcgtcg
     1321 tcgaccaggt cgcggcgcat gcgcttgagc acgaccgccc gctcgagcgc cggttggcgc
     1381 gcgaggtaga ccacgcccat gccgcccgag ccgatctcct cgacgatctc gtagctgccc
     1441 agcttcctgg gccgcgtgcg cgcgccttcg gtcgacaccg gtcgcccctc ggatggaatc
     1501 agcgctctgt ggacaggtcc cagacctcgg gtggagtctc gcgctcttcg acgaccagct
     1561 ggaagacgag cgtgcccatc tcgatgtggt cgccgttgtc gagatcgcac agctggactc
     1621 ggttgccgtt gacgcgcagg ccgttggtgc tgcccaggtc gcggatgcga tagcctccgt
     1681 cctcgaagtc gaaggcggcg tgctggcgcg acaacgccgc atcatcgaac gccaggtcga
     1741 ccccgggccc gcggccgagc gtgacgcgcc gccgatcgag ctcgtaggcc gcgccggccc
     1801 gcggaccgct gaggaccacg accttgaacg agcggcccaa gacggaatcg tcggtccggg
     1861 ccggtcgcgt tttcagcttg cgcgtgagtc cgtcctgcat gccgtcctcc tggcgggcac
     1921 ccggggccct gcctggaatg ggtaggtccg cactaaagga aagtcaagaa aacgggcacg
     1981 gcgggcgatt cgccgctacg gcttgcccgc gaccggccgc agctcgatgt ggaatcccac
     2041 ccgccgcccg tcgcgcagcg cctcgacgtc gatgcgcaat cggtcggggg cgacgcggtg
     2101 gtaggtcagg atctgcggaa agtcgtgctc ggggttggtg aagaccgccc gccgcgcctc
     2161 ggcatcgagc tcggtcagcg tgaagtgcac ttcgctcgga cgcccgccgg gtgagggcat
     2221 catcaccacc tggccctcgc gcacgacgaa gcgctcgaac tcgatcgacg cgacgcggcc
     2281 gtcgacgatc tgcttgttcg cgctgaggat cgtcccaccg tcggcgccgg tgtaggccgc
     2341 ctcccactcg ccctcgtcgg ccgggccccg ccagtgcccc gcgagccagc cgagcgaggc
     2401 gagccgcctc gacgcctcct cgtcgcccgc ggcgtccgcg cggagcggac tgccgtcgag
     2461 cgacagggca gtcaacagaa cggcggtcgc gatgaacgtc cgcatcgggg tatcctcctg
     2521 ccgtccttta caatacttta ccggccggcg gcgcacccct gacgtagtac ccgccgagga
     2581 ggaatgaaga tgggacccga ccgtgcggac cgcttcggca tcgcgacgct cgcgctgctc
     2641 gccttaggcc tggtgtcgac ggcgaccgcc gacgactggc cgcaatggcg tggccccgag
     2701 cgcgacggcc gctcggccga aacggggttg ctcgacaact ggccggcggg cggcccgccg
     2761 ctcgagtggc gcgcttcagg gttgggcagc gggttctcga gtgtcgccgt cgcaggcgag
     2821 cgcatctaca cgctcggtga ctcgggtgat gcgcaatacg ccgtggcgct cgaccgcaag
     2881 gacggcaagc agctgtggaa gacacgcgtc ggcccggcgt gggacgacga gtacctcggt
     2941 ccgcgctcga caccgaccgt cgacggcaag cggatcttcg tcctgtcgac cgaaggcgac
     3001 ctgttcgcga tcgacgccga gagcggcgtg acggtctggc agcgcaacct ggtgaaggac
     3061 ttcggcggcg cgatgatgaa ggcgatgggc cagtacgaat ggaagtacag cgaatcgccg
     3121 ctggtcgacg gtgaccgcgt cgtcgtcacg ccgggaggca aggaagcgat gatggtggcg
     3181 ctcgccaagg ccacgggcga ggagatctgg cgcgtcgcga tcccgtcgct cggcgagaag
     3241 ggcacggacg gcgccggcta ctcgtcgccg gtcgtctccg aggccggtgg cgtgcggcag
     3301 tacgtgcagt tcgtcgggcg cggcgtgatc ggcgtcgagg cgaagaccgg ccgcttcctc
     3361 tggggctaca acaaggtcgc gaacgacgtc gcgaacatcc cgacaccgct ggtcgacggc
     3421 gatcgcgtct tcgcctcgag cggctacggc accggatcgg cgttgatcga gctgtcgcgc
     3481 ggcgacggcg gcttctccgc gcgcgaggtc tacttcctcg gaccggacac gatgcagaac
     3541 caccacggtg gactgatcct gcacgacggc cacgtctacg ccggctcggg ccacaacaag
     3601 gggctgccgg tcagcgtcga gctggcgacg ggcaaggtcg cctggggtcc cgagcgcaac
     3661 gacggccagg cctccgccgc gatcgcctac gccgacgggc ggctctactt ccgctatcag
     3721 aacgggctga tggtattggt cgaggcgacg ccggagggct accgcgagca cggctcgttc
     3781 acgatccccg acgtgaaacg accgagctgg ccccatccgg tgatcgccgg cggcaagctc
     3841 tacctgcgcg agcaggacca cctctactgc tacgacgtgc gcgcccgagg ctgagatcga
     3901 ccgttccggt cggttggtcg gcggcgaccc gggtgtaacc tgtccgcgtc cgggatctcc
     3961 cggaggtgga gggtcgttcc agtgacgggt cgcgccgcct cgggtcgagc ggtcttcatc
     4021 gtcgatgccc ggcgcacgcc gttcctgcgg tcgggcagcg agtttcgcga gctgtgcgcc
     4081 tacgacctgg ggcgcatggc gatcgcgggc ctgctgcata agagcggcgt cgatcgcacg
     4141 acgatcgacc gcgtggtgct gggaacggtc gtcgccgatc cggacaccag caacgtcgcg
     4201 cgcgagtcgg ccctggccgg cggcgtcccg gcgagctgcc cggccaacac ggtgaccgcc
     4261 gcgtgcatct cggccaacat cgccttcgag caggcggccc acgcgatcgc gaccggggag
     4321 gccgacgtgg cgatcgccgg cggcaccgag acgctgtcgg acgtgccgat ccgcgtcagc
     4381 cgcgcggtgc gcaagcggct gatcgcggcg cggaaggtgc gcggcatcgg cggctggctc
     4441 gccttgctgc gcgggctccg cccgcgcgat ctgctacccg tcgtcccggc gatcgccgat
     4501 ttctcgaccg gcctgacgat gggtcagaac gccgagcgtc tggccaagcg gatcggaatc
     4561 tcgcggcacg accaggacga gtacgcgatg ctgtcgcacc accgcgcggc cgaagccgcg
     4621 ggtcgcggtt ggctcgactc gcagatcgtc ccggcgcggc tgccgcgcga gttccgcccg
     4681 atcgatcggg acaacggcat tcgcggcgat acgtcgatgg agaaactggc cgagctgaag
     4741 ccggcgttcg accgtcgctt cggcaccatc accgccggca acagctccta cctgaccgac
     4801 ggtgcatcgg ccgtgctgct ggcgagcgag gacgggctcg ccgagaccgg cctcccgccg
     4861 ctcgcgcgcg tggtgagcca agcggtaagt gcgatggatc cgctcgaaga gctgctgctc
     4921 ggtccgtcgt tcgcgatccc cgcagcgctc gatcgcgcgg gtctgacgct cggcgacctc
     4981 gacgtcatcg agctgcacga ggcgttcgcc gcgcaggtgc tggcggtcat tcgattgctg
     5041 gccgacgcgc gcttcgctcg cgacagcctg tcgcgcgatc gcgccgtcgg gcggatcgac
     5101 atcgaacgga ccaaccgctg gggcggctcg ctgtcggtcg gccacccgtt cggcgcgacg
     5161 ggtggccgcc tggtcgccaa ctgctgccgg cggctgatcc acgaggacgg ccggttcggc
     5221 ctcgtcgcgg cctgcgcctc gggcgcgctg ggctacgcga tggtcgtcga gagggtcggc
     5281 tgatggcgcc gggcgcggtc cgactcgaca tccgcgccga cggcgtggcg atcgtcacct
     5341 tcgacacacc ggagagcaag gtcaacatcc tgtctcgcga gctgttcgac gagatcgcca
     5401 ccgtgctcga ccggatcgag tcggagcgcg ccgtccgcgc cgcggtgctc tcgagcggca
     5461 agccggggag cttcatcgcc ggtgcgaacc tcgagcagtt gaagtcgatc gaccgcgagg
     5521 aggaaggacg gcgcttctcg agcgacggcc agcgattgct cgatcgcatc gcggagagcc
     5581 cgaagccgtt cgtcgccgcg atccacggcc cggcgttggg tggtggtctc gaggtcgctc
     5641 tcgcctgcca ctacatcgtc gcctcggatt cgccgtcgac ggtgctggcg ctgcccgagg
     5701 tgatgctcgg cctgctgccg ggaggcggcg gcacgcaacg cctgccgcga cgcgtcgggc
     5761 tggtcgaggc gctgccgttg atgctcaccg gccggcgtct ccgcgcgcgc cgggcctacc
     5821 gcatcgggct ggtcgacgcg gtgacctcgc cgggcgggat cgccgagacc gcggcccgcg
     5881 ccgcctcgat gctggccgac ggcaagctgt cgcgtcgtcg ggtcagccgc ccgctggcca
     5941 accggctgct cgagatcgcc ccgttccgtg cgctcgtctt ccgtcgcgcg cgcgcccagg
     6001 ttcgtcgcca gacgcgcggc aattatccgg cgccgccgtt catcgtcgac tgcgccgcga
     6061 ccggct
//
LOCUS       DASPGN010000611         6227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1049700, whole genome shotgun sequence.
ACCESSION   DASPGN010000611 DASPGN010000000
VERSION     DASPGN010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049700"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VD788_17750"
     CDS             <1..1268
                     /locus_tag="VD788_17750"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HXV78159.1"
                     /translation="RDEQDRLRHTEASGAVKAHVLLEPDIPGDVAGDHLEVDWDERRE
                     VERLLLEGNASLSRAGESLTADRVAAVREATGGGWELTATGTVFAQGSLGGQPAWIRS
                     ERLDATVDRTRQLRSAIAQGRVRFEAPATRAEADRAEFHPTAEGGEVHLFAAAGRKAR
                     LAREQTRVAAETIRSDLAGRRLDAEQRVEATLMPVENRGAGGADGLFRDGQAVHFVSE
                     KLVGENSGERLVFSGGVRGWQGERNLSADRIEMRRSTHSLSARGGVTTRAPREADGGA
                     LGESDYVQIAADQLDYDGVSRLATYRGGARVTLVEGWLEADRIEVRLSPDEGGVTEVW
                     AFDDVRIEFSDPERSDRTEIIAGAADRVSYLPADRSVRLFGDDAPATVRRLGPQGATT
                     SGRVLRYQLDVGTLEVESGQPAPARIRGR"
     gene            1265..2053
                     /gene="lptB"
                     /locus_tag="VD788_17755"
     CDS             1265..2053
                     /gene="lptB"
                     /locus_tag="VD788_17755"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXV78160.1"
                     /translation="MSESEVSRLSEVAIGAPEAGDKHGLVARDLVKQFRGRKVVDGVS
                     VHIRPGEVIGLLGPNGAGKTTSFHMILGLMPPKRGRVLLDGKDITRLPMYMRARRGIG
                     YLPQEASIFRRMSVEENLLSVFEMRRVRSEEAARRTKELLQEFRLTKVARSPGYALSG
                     GERRRAEIARALASEPRYMLLDEPFAGIDPIAVNDLQAIVVRLKEKGLGVLITDHNVR
                     ETLQITDRAYIISQGNIFRHGTPSELASDAEVRKVYLGQNFKLH"
     gene            2075..3514
                     /gene="rpoN"
                     /locus_tag="VD788_17760"
     CDS             2075..3514
                     /gene="rpoN"
                     /locus_tag="VD788_17760"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HXV78161.1"
                     /translation="MALEQKLSVRMSQRLVMTPSLQQAIKLLQMSKLELVEEVQQEML
                     ENPVLEEQSEAPQSERQEGESPEPVEAQEPSETADPFDDIDYESYFEDVENNYAPRAP
                     REAGEELPSFEATLASKPNLADHLTWQLDLSVSDATTKEIGRAIIGNINEDGYLRATL
                     AEIRQLGSWSMEQTEATLRIIQGFDPVGVASHDLVECLCLQLVALGEDESPAGTIVRY
                     HLDKLQNRRFKELAEILELEMDDLQAEIEIIRGLDPRPGQKYNNVSSTYVVPDVYVIK
                     IDEDYQIVLNEEGLPRLRISPVYRRMLSRHAPGTAPSDAKEYVRNKLRSAFRLIKSLE
                     ERQRTIYKVAQSIVKFQREFLDYGIERLRPLVLKDVADDIGMHESTVSRVVNNKYMHT
                     HRGLFEMRFFFHSGIASTRGEPSVSSLTVKEKIRKIVAAEDARKPFSDSAIVKLLKND
                     GLQIARRTVAKYREELKIPSSSNRKQVFR"
     gene            3533..4099
                     /gene="raiA"
                     /locus_tag="VD788_17765"
     CDS             3533..4099
                     /gene="raiA"
                     /locus_tag="VD788_17765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXV78162.1"
                     /translation="MQLEITGRHIEITPALREFTEEKLGKLARLLDGPLEVHVVLGIE
                     KHRHLAEVQVKSKYAVFAGAQETGDLYASIGEVADKLERQVLKHKEKMRTRKVKRGPR
                     RPEAAAEMEAKASPAGPGDGNERPRRTQPSVAARRYRVKPLSPEDAMLELESTKGDVL
                     VYRDAQTDRVGVVYRQKDGSLALIEPEY"
     gene            4251..5945
                     /locus_tag="VD788_17770"
     CDS             4251..5945
                     /locus_tag="VD788_17770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXV78163.1"
                     /translation="MSKPDLTNEAAARRSTSRAEWERQTLRPNLDASGERPVEFSTVS
                     SLPIERLYTAEELAAGWDVNEKLGYPGEFPYTRGIHPTMYRGRTWTMRMFAGFGSAEQ
                     TNRRFKYLLDHGQTGLSTAFDLPTLMGRDSDDPMSRGEVGREGVAIDTLADMEQLFNG
                     IPLDRVSTSMTINAPAAVLLAMYLAVADKQGVSFEKLNGTLQNDILKEYIAQKEWIYP
                     PRPSMRIITDLIAFCSRHVPKWNTVSISGYHIREAGSTAVQELAFTLADGIGYVQAGI
                     DAGLDVDVFAPRLSFFFNSHNDFFEEIAKLRAARRMWAHIMRDRFGAKNPRSWTLRFH
                     TQTAGCSLTAQQPINNVVRVTIQALAGVLGGTQSLHTDSYDEALALPSDEAVTVALRT
                     QQIIAEESGVTNTVDPLGGSYFVEQLTDRMERDALAYIDRIDEMGGIVEAIEAGFPQK
                     EIADASYRYQRQVDDGSKVVVGVNKYRMEEARGVETLRIGDDVERGQLDRLARVRAGR
                     DEAAVRRMLDAVTRAADNGDNLIPPLLDAVRVYASVGEVCRALVPVFGTYQETSVL"
     gene            5962..>6227
                     /locus_tag="VD788_17775"
     CDS             5962..>6227
                     /locus_tag="VD788_17775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXV78164.1"
                     /translation="MSEGPKIRLLVGKVGLDGHDRGAKIIARAFRDAGFEVIYTGLHQ
                     TPEVVVNTAIQEDVDAVSLSILSGAHNYLFPRVIELLREAGADE"
BASE COUNT         1170 a         1955 c         2137 g          965 t
ORIGIN      
        1 tgcgcgacga gcaggaccgg ctgcgccata ccgaagcgag cggggcggtc aaggcccacg
       61 ttctgctcga gcccgacata ccgggagacg tcgccgggga tcatctcgag gtcgattggg
      121 acgaacgccg ggaggtggag cggttgctgc tcgaaggaaa cgcgagtctc tcgcgcgcgg
      181 gggagtcgct gaccgccgat cgcgttgccg cagtgcgaga agcgaccggc ggtggttggg
      241 agctgaccgc cacgggaacg gttttcgcgc aaggatcgct cggcgggcag ccggcatgga
      301 tccgctccga gcgcctcgac gccaccgtcg atcgcacccg gcagctgcgc tcggcgatcg
      361 cgcaggggcg agtgcgcttc gaagcgccgg ccacgcgtgc cgaggccgat cgcgccgagt
      421 tccatccgac ggccgagggc ggcgaggtgc acctgttcgc cgccgcggga cgcaaggcgc
      481 gtctggcccg cgagcagacg cgcgtcgccg ccgagacgat ccgctccgat ctcgccggac
      541 ggcgtctcga cgccgagcag cgcgtcgagg cgacgctgat gccggtcgaa aaccgcggcg
      601 ccggcggggc cgacggattg ttccgcgacg gccaggccgt gcacttcgtc tcggagaagc
      661 tcgtcggcga gaactccgga gagcgtctgg tgttcagcgg cggtgtgcgg ggttggcagg
      721 gtgagcgcaa tctgtcggcc gatcggatcg agatgaggcg cagcacacat tcgctctcgg
      781 cgcgcggggg cgtgacgacc cgcgcccctc gcgaggccga cggcggcgcc ctcggcgagt
      841 cggactacgt ccagatcgcg gccgaccagc tcgactacga cggcgtctcc cgtctggcga
      901 cttaccgtgg tggcgcgcgg gtgacgctgg tcgaaggctg gctcgaggct gaccggatcg
      961 aggtccgcct ttcccccgac gaaggcggtg tcaccgaagt ctgggccttc gacgacgtgc
     1021 gcatcgagtt cagcgaccct gagcgatcgg accggaccga gatcatcgcg ggagccgcgg
     1081 atcgggtttc ctaccttccg gccgacagga gcgtgcggtt gttcggcgac gacgcgccgg
     1141 caaccgtccg ccggctcggc ccgcaggggg cgaccaccag cgggcgggtg ctgcgctacc
     1201 agctcgacgt tggtactctt gaagtcgagt cgggccaacc ggctccggcg cgaatccgcg
     1261 gacgatgagc gagagcgagg tcagtcgatt gtcggaagtc gcgatcggcg cgccggaggc
     1321 gggggacaag cacggtctgg tggctcgcga tctggtgaag cagtttcgtg ggcgcaaggt
     1381 ggtcgacggc gtctcggtcc acatccgacc cggcgaggtc atcggcttgc tcggtccgaa
     1441 cggcgcggga aagacgacgt cgtttcacat gatcctcggc ctgatgccgc ccaagcgtgg
     1501 gcgtgttctg ctcgacggga aggacatcac gcggctgccg atgtacatgc gcgcccgccg
     1561 cgggatcggc tacctcccgc aggaggcgtc gatcttccgc cggatgtccg tcgaagaaaa
     1621 cctgctctcg gtgttcgaga tgcgtcgcgt acgcagcgaa gaggcagcgc gacggacgaa
     1681 ggaactgctg caggagttcc gcctgaccaa ggtcgcacgc tcgccggggt acgcactgtc
     1741 aggcggcgag cgacggcggg ccgagatcgc ccgtgcgctg gcgagcgagc cgcgctacat
     1801 gttgctcgat gaaccgttcg ccggcatcga tccgatcgcg gtcaacgacc tgcaggcgat
     1861 cgtcgtgcgg ttgaaagaga aaggtctcgg cgttttgatc accgaccaca acgtgcgcga
     1921 gactttacag attacggacc gcgcatatat tatcagccag gggaatattt tcaggcacgg
     1981 caccccgtca gagctcgctt cggatgcgga agttcgcaag gtctatctcg ggcagaactt
     2041 caagctgcac tgacgcgcgg cttgaaagga gtcgatggcg ctagaacaaa aactgagcgt
     2101 ccgcatgagc caacggctcg tgatgacacc ctcgctgcag caggcgatca agttgctgca
     2161 gatgtcgaag ctcgaactcg tcgaggaagt ccagcaggag atgctcgaga atccggtgct
     2221 ggaggagcaa tcggaagcgc cgcagagcga acgtcaggag ggtgaatcgc cagaaccggt
     2281 cgaggcccag gagccgagcg agacggccga tccgttcgac gacatcgact acgaatccta
     2341 tttcgaggac gtcgagaaca actacgcccc gcgcgcgccg cgtgaagccg gcgaggaact
     2401 accgtcgttc gaggccacgt tggccagcaa accaaatctg gccgaccacc tcacctggca
     2461 gctcgacctg tccgtctccg acgcgacgac caaagagatc gggcgcgcga tcatcggcaa
     2521 catcaacgaa gacggctacc tccgtgcgac gctggccgag atccggcagc tcggcagctg
     2581 gtcgatggag cagaccgagg cgacgctccg catcatccag ggcttcgatc cggtcggcgt
     2641 cgccagccac gacctggtcg aatgcctgtg cctgcagctc gttgcgctcg gtgaggacga
     2701 gtcgccggcc ggcacgatcg ttcgctacca cctcgacaag ctgcagaacc gccgattcaa
     2761 ggagctggcc gagatcctcg agctcgagat ggacgacctg caggccgaga tcgagatcat
     2821 tcgcggcctc gacccgcgac cgggacagaa gtacaacaac gtcagcagca cctatgtcgt
     2881 ccccgacgtc tacgtgatca agatcgacga agactatcag atcgtgctga acgaagaggg
     2941 gttgccgcgc ctcagaatca gtccggtcta ccgccgcatg ctgtcgcgcc atgctccggg
     3001 gacggcaccg tccgacgcca aggagtatgt ccgcaacaag ctgaggtccg cgtttcgctt
     3061 gatcaagagc ctcgaggaac gccagcgaac gatctacaag gtcgcccagt cgatcgtcaa
     3121 gttccagcgc gagttcctcg attacggcat cgaacggctg cgcccgctcg tgctgaaaga
     3181 cgtggccgat gacatcggca tgcacgaatc gaccgtcagc cgcgtcgtca acaacaagta
     3241 catgcacacg caccggggcc tgttcgagat gcggtttttc tttcacagcg gtatcgcctc
     3301 gacccggggc gaacccagcg tctcgtcgct gactgtcaag gagaagatcc gcaagattgt
     3361 cgctgccgag gacgcgcgaa agccgttctc ggattcggcg atcgtcaagt tgttgaagaa
     3421 cgacggtctg cagatcgctc gccgaacggt ggccaagtac agagaggagc tcaagatacc
     3481 ctcgtcgagc aaccgaaagc aggttttccg ctagggccaa ggagaggttc gcatgcaact
     3541 ggaaatcacg ggacggcaca tcgagatcac accggctctg cgtgagttca ccgaggagaa
     3601 gctgggcaag ctggcccggc tgctcgacgg accgttggag gtccacgtgg tgctcgggat
     3661 cgagaagcac cgtcaccttg ccgaggtcca ggtcaagtcg aagtatgcgg tcttcgccgg
     3721 ggcgcaggag accggcgatc tgtatgcctc gatcggcgag gtcgccgaca agctggagcg
     3781 gcaggttctc aagcacaagg agaagatgcg gacgcgcaag gtcaaacgcg gaccgcgacg
     3841 gccggaggcg gcggccgaga tggaggccaa ggcatcgccg gccggacccg gcgacgggaa
     3901 cgagcgcccg cgccgcacgc aaccgtcggt cgcggctcgc cgctaccgcg tcaagccgct
     3961 gtcaccggaa gacgcgatgc tcgaactcga gagcaccaag ggcgacgtgc tggtctatcg
     4021 cgatgcccag accgaccgcg tcggcgtggt ttatcggcag aaagacggga gtctcgcgct
     4081 gatcgaaccc gagtactgac gcgggccggg cggactcgcg ggcttgaccg ttccggggca
     4141 cgctgataag ttttagaatc gaaacgactt gcggaaccag tcggaggggt gttgcctgcc
     4201 gggcgagcgg caacgacccc gggcggctcc gagcggcaga ggcggtagcc atgagcaaac
     4261 ccgacctgac gaacgaggca gcggcgcggc gcagcaccag tcgcgcggaa tgggagcggc
     4321 agacgctgcg tccgaacctc gacgcgagcg gggagcggcc ggtcgagttc agcacggtgt
     4381 ccagtctccc gatcgagcga ctgtacaccg cggaagagct cgccgccggt tgggacgtga
     4441 acgaaaagct gggttacccc ggcgagttcc cctacacccg cgggatccat ccgacgatgt
     4501 accgtggccg gacgtggacg atgcggatgt tcgccggctt cggttccgcg gagcagacga
     4561 accggcgctt caagtacctg ctcgaccacg gccagaccgg cttgtcgacc gcgttcgacc
     4621 tgccgacact gatgggtcgt gacagtgacg acccgatgtc gcgcggcgag gtcggccgcg
     4681 agggtgttgc gatcgacacg ctggccgaca tggagcagtt gttcaacggc atcccgctcg
     4741 atcgcgtctc cacctcgatg acgatcaacg cgcccgcggc cgtcttgctg gcgatgtatc
     4801 tggccgtcgc cgataagcag ggagtctcgt tcgagaagct caacggaacg ctgcagaacg
     4861 acatcctcaa ggagtacatc gcccagaagg agtggatcta cccgccacgg ccgagcatgc
     4921 gcatcatcac cgatttgatc gcgttctgct cacgccacgt gccgaagtgg aacaccgtct
     4981 cgatctcggg ctaccacatc cgcgaggccg gctcgaccgc cgtccaggaa ctggccttca
     5041 cgctggccga cggcatcggc tacgtgcagg ccggcatcga cgcagggctc gacgtcgacg
     5101 tcttcgcccc gcggttgtcc ttcttcttca attcccataa cgacttcttc gaggagatcg
     5161 ccaagttgag ggccgcccgg cggatgtggg cccacatcat gcgtgatcga ttcggagcga
     5221 agaacccgcg atcgtggacg ttgcgcttcc acacccagac cgccggctgc tcgttgaccg
     5281 cccagcagcc gatcaacaac gtcgtacgcg tcacgatcca agcgctggcg ggtgtgctag
     5341 gcggcaccca gtcgctgcac acggactcct acgacgaggc gctcgcgctg ccgtcggacg
     5401 aggcggtgac cgtcgccctg cggacccagc agatcatcgc ggaggaatcc ggggtgacca
     5461 acaccgtcga tccgctcggc ggctcgtact tcgtcgaaca gctgaccgac cggatggagc
     5521 gcgacgcgtt ggcctacatc gaccggatcg acgagatggg cgggatcgtc gaggcgatcg
     5581 aggccggctt cccgcagaag gagatcgccg acgcctccta ccgctatcaa cggcaggtcg
     5641 acgacggtag taaggtcgtc gtcggcgtca acaagtatcg catggaagag gcgcgtggtg
     5701 tcgagacgct caggatcggc gacgacgtcg aacggggaca gctcgaccgg ctggcgcggg
     5761 tgcgcgccgg gcgagacgag gccgcggtgc gcaggatgct cgacgccgtg acgcgagccg
     5821 ccgacaacgg tgacaacctg atcccgccgc tgctcgacgc cgtgcgtgtc tacgccagcg
     5881 tcggcgaggt ttgtcgcgcg ctggttccgg tgttcggtac gtaccaggaa acgtcggtgc
     5941 tgtagcgcgc ggagacggac ggtgagcgag gggcctaaga tccgtttgct ggtgggcaag
     6001 gtggggctgg acggtcacga ccgcggagcc aagatcatcg cgcgcgcctt ccgcgatgcg
     6061 ggtttcgagg tgatctacac cgggctgcat cagaccccgg aggtggtcgt caacacggcg
     6121 atccaggaag acgtcgacgc ggtgtcgctg tcgatcttgt cgggggccca caactacctg
     6181 ttcccccggg tcatcgagct gttgcgcgag gcgggggctg acgagat
//
LOCUS       DASPGN010000612         9273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1049923, whole genome shotgun sequence.
ACCESSION   DASPGN010000612 DASPGN010000000
VERSION     DASPGN010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049923"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VD788_17780"
     CDS             <1..280
                     /locus_tag="VD788_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78165.1"
                     /translation="FEPELFEALGRGYLAVARALLVTAEIDHLVDASIALTFVAGLRF
                     LTDHLAGDRYFRATRPGQNLDRARVQFVLVEQMERAVGPLRAAIARCR"
     gene            482..1135
                     /locus_tag="VD788_17785"
     CDS             482..1135
                     /locus_tag="VD788_17785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78166.1"
                     /translation="MALRGTLGLLLVWLPFATVHAEGHQLVERWREAEFTPRKFSKVI
                     VLAVTDDRQLRKTFENRFVSHLRGQQIECTTSYAIAPDLTGIDEAERRVIVDTVEAEK
                     IDGVIAIRAVPLIGLDEQQWAEQWRERINRRQSLAETIEQSLPLAETKAKRYGIEVEL
                     WAVPDAGRVWAARTNPYTRDEMRKGAAEFVQFTLYALDIEDLMKRGAAAGEDSNAKP"
     gene            1135..2196
                     /gene="pip"
                     /locus_tag="VD788_17790"
     CDS             1135..2196
                     /gene="pip"
                     /locus_tag="VD788_17790"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HXV78167.1"
                     /translation="MRRSGARCLFAGALALCIGCGGSPPSELFPPVEPTETGYLRVDE
                     THELYWETIGNRQGIPVIVLHGGPGGSASPETRRLFDPERFFVLLFDQRGAYRSRPQG
                     EWRDNTTAHLVEDINRLRDHVGLTGPVLLFGGSWGATLALAYAEKYPERVNGLVLRGV
                     FLGSSEEIDYLYHGGAGAFFPETWERLRNLVPQPDVPDYPRQLFEMISGGGAKDREAA
                     IEGWAYYEIRMSSLEMTDDRAEQAVRDYHDQLMPFSLLENYYMMNGCFLDDRQLLDHA
                     DRIADIPTFIVHGRYDAICRPVVAWSLAQRLDNVELVFTHAGHSQSDPANTDALLAGL
                     DWVADRIEADGLRPQIADR"
     gene            complement(2211..3932)
                     /locus_tag="VD788_17795"
     CDS             complement(2211..3932)
                     /locus_tag="VD788_17795"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXV78168.1"
                     /translation="MSQQFREVTTWLREQVVGGHTRIQTPFGPRLIYYADLTATGRHL
                     AFVDEWVNRLSPYYANTHTAVSSTGNLLTELRATARAIIAQAVNASNQDEVVFVGSGA
                     TAAANKLVGLLGWRISEPLERVHRLGQAIPAAARPVVFVGPYEHHSNLLPWLESIAEV
                     VEIGADSSGKISLGELEARLAQYSARETKVGSFSAASNVTGVLSDVRAIARVLHRNGA
                     LAFFDYAAAAPYVPIDMHPGTDDERVDALFLSPHKFLGGPGSSGVLVARRELFRSRVP
                     ERPGGGTVDYVAGARRDAVDYVTGLAEREEGGTPAIAGDLRAGASFLVKHAIGPQEIL
                     EHEMRLAEAAIARLAQHRKIKVLGPRDLPRLAILSLSIDGLHHDLASALLDHLFGIQN
                     RAGCSCAGPYGHDLLGIRPEASARFRRLVRRGLNGMKPGWVRLSLPYYANPEDIEFVL
                     RAIEFVADHGIDFVPLYRLNWRDGVWRHIDGVVPPSPGIELSIEALEDAADNPRGGVY
                     ERRLGETALREERQRYFDEARDLAVRLGARFKVTRPRWNLPTGDAEIDELVWFRYVHA
                     EGLSDVP"
     gene            4206..4454
                     /locus_tag="VD788_17800"
     CDS             4206..4454
                     /locus_tag="VD788_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78169.1"
                     /translation="MQSLLSFKGCTLIDSSLIRSSGESQVSVGPRCRSIGSSSRRRSA
                     TAGPATSFSTAVGETVVVGTSRPMESHGALVVLLTALE"
     gene            complement(4434..5645)
                     /locus_tag="VD788_17805"
     CDS             complement(4434..5645)
                     /locus_tag="VD788_17805"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HXV78170.1"
                     /translation="MPNRTAMARIAICSILSLPWAPTASWSQQCVDVPSGLIGWWPGD
                     GSGEDLARGVDGALLGEVGFGDGVVGQAFVFDGIDAGIEVTNADGAFDLVEAWTVDAW
                     VFPAADGQDARLDPIVWKIGAPNTNNDTFDLGWGCHDCAGRNRFQTSTERASDGQDFV
                     VTSAEHPAASWYHIAAVYDGINLLLYVDAVEEGRALIGQVTAYTGPAPLRIGNILHTS
                     HSPRGVFDGRIDEVEIFDRALTPDEIRAIHQAGPAGKCKSHDDDGDGFTAPVDCDDED
                     AAVHPDAAELPGNRLDENCDGLVSCDPCESWRNHGHYVSCVAREVAVLVDEGRIDAEH
                     GEELVSTAARSEVGKRVGAPDECSLARVQAAVATRRGRFGAVHPPSAPRPRHVLQTDA
                     DVSAGSFERGK"
     gene            complement(5861..6112)
                     /locus_tag="VD788_17810"
     CDS             complement(5861..6112)
                     /locus_tag="VD788_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78171.1"
                     /translation="MRGRKRWVTPHVATRLVRQVVEHPELLENAEARQIVAELQELER
                     KLRRLDDRASDDRVRARMRSDMLLGMARGWSRLNRYLGD"
     gene            6247..7176
                     /locus_tag="VD788_17815"
     CDS             6247..7176
                     /locus_tag="VD788_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78172.1"
                     /translation="MTDERRIESIPGVCDVERLGRSAGAPTLLIELPHGATRGSDFDA
                     LAARLISMLPDRLREFFFVNTDVGCHEAARLIAARLVDHFRDGPAVEVVIVRSLLPRT
                     LVDCNRLLAASADDARRMTPAIPSYVDRREDRVMLADLYARYRRLVERAYEALDADRG
                     WALQLHTYAPRSIQIDTIDGSIVETLREAYAPENYDRWRSRPSVELISEDPAGVMLAP
                     ARLVTAIKRHYRAAGIELAENETYRLHHDTTGYVDAARRPGRVCCVELNRGLLAEPFA
                     PFEEMRVGAAKLERLAGPLASAYIEIIEEGAGA"
     gene            7173..8837
                     /locus_tag="VD788_17820"
     CDS             7173..8837
                     /locus_tag="VD788_17820"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXV78173.1"
                     /translation="MNKFFVSIGAGLATILAAGPAPAQTVTDPSVNLEVESVVEGLFL
                     PTSMAFVGDEEILVLQKNDGLVRHVVGGVLQPGSVLDVDVHVQSERGLLGIATDPDFI
                     HTPYVYLYFTESSTGGDTNDGNSIPLGNRVYRYSWNGSGLVDPLLVLDLPATPGPNHD
                     GGIVVFGPDDALYAVIGDLNRNGKLQNYPAGPEPDDSGVILRVHRSGATLTDGPFRDA
                     GDPGNPLNRYWAYGVRNSFGMAFDPVTGELWNTENGPASYDEVNRVPPGFNSGWERIM
                     GPDARDADDQDDLWVAPGSAYRDPEFSWASTVAPAAIAFVASRQLGCELEHDVIVGDD
                     NCQQLYRFEPNAARDGLVFASPELADRVADNGLLRCSSEQSEVLFGSSWGTVTDIENG
                     PDGYLYIVSLTQGRILRLVPDHGTFDDADSDGVEDACDCAAGDSTAFAEPGEVPRIRL
                     GAATPTTLGWDPQTATAGKGTTYQVVSGDLDSLRAAGGFAGACELAAGLATPALGDPR
                     ADPAPGTGVYYLTRAGNACDAGTFGDGSAPADPRDLLDAGTPPACP"
BASE COUNT         1463 a         3169 c         3184 g         1457 t
ORIGIN      
        1 cttcgagccc gagctgttcg aggcgttggg ccgcggctat ctcgccgtgg cgcgcgcact
       61 gctggtcacg gccgagatcg atcatctcgt cgacgcgtcg atcgcgctga ccttcgtcgc
      121 cggtctgcgc ttcctgaccg accatctggc tggcgaccgg tacttccgcg cgacacgacc
      181 ggggcagaat ctcgaccgag cgcgggtcca gttcgtgttg gtcgagcaga tggagcgcgc
      241 cgtcggtccg ctgcgcgccg cgatcgcgcg ctgccgctag accccagacg agaaaagccc
      301 cgagagcggg ctctcggggc tcgggacggt gtcgtaggag cttggggggt tctctacgac
      361 gcgccgacca gcttatgttc ctacgccacg ctccgcgctg attcgcggcg ccaaaatcgt
      421 gggcaatcgc gccagccagc ggataagatg gtcgttccgt tcgcccggcg ggggtgggaa
      481 catggcgctg cgtgggacgt tgggcttgct tctcgtctgg ttgccgttcg cgaccgttca
      541 cgccgagggg caccagctcg tcgagcgctg gagggaggcg gagttcacgc cgcggaagtt
      601 ctccaaggtg atcgtgctcg ccgtcaccga cgaccggcag cttcgcaaga ccttcgagaa
      661 tcggttcgtc agccacctca gaggccagca gatcgagtgc acgaccagct acgcgatcgc
      721 tcccgacctg accggcatcg acgaggccga gcgccgagtc atcgtcgaca cggtcgaggc
      781 cgagaagatc gacggcgtga tcgcgatccg tgccgtgccg ttgatcggtc tcgacgagca
      841 gcaatgggcc gagcagtggc gagagcggat caaccggcgt cagagcttgg ccgagacgat
      901 cgagcagagc ctgccgttgg ccgagacgaa ggccaaacgt tacgggatcg aggtcgagct
      961 gtgggccgta cccgacgccg gtcgcgtgtg ggccgcccgg accaacccct acacccgcga
     1021 cgagatgcgc aagggtgccg ccgagttcgt ccagttcacc ctgtacgctc tcgacatcga
     1081 agatctgatg aagcgcggtg cggcggccgg cgaagattcg aacgcaaagc cctgatgcgg
     1141 cgctcgggag cccgctgcct gttcgccggc gcactcgcac tgtgcatcgg ctgcggcggc
     1201 agtccgccgt ccgagctgtt cccgccggtc gagccgacgg agaccggcta cctgagggtc
     1261 gacgaaacgc acgagctgta ctgggagacg atcggcaacc gccaggggat cccggtgatc
     1321 gtgctgcacg gcggcccggg tggctccgcg tcgcccgaga cacgccgact gttcgatccc
     1381 gagcggttct tcgtgctgct gttcgatcag cgcggcgcgt accgcagtcg gccgcagggc
     1441 gaatggcgtg acaacaccac ggcgcacctg gtcgaggata tcaaccgact gcgtgaccac
     1501 gtcggtctga ccgggcccgt gttgttgttc ggcggctcgt ggggcgccac gctggcgctg
     1561 gcctacgccg agaagtatcc cgagcgggtc aacgggctcg tgctgcgcgg cgtcttcctc
     1621 ggttcgagcg aggagatcga ctacttgtac cacggcgggg ccggggcgtt ctttcccgag
     1681 acctgggagc ggctgcgcaa tctggtcccg cagccggatg taccggacta tccacgccag
     1741 ttgttcgaga tgatcagcgg cggcggcgcc aaggaccgcg aggcggcgat cgagggctgg
     1801 gcttactacg agatccggat gtcgtcgctc gagatgaccg acgatcgcgc ggagcaggcg
     1861 gtgcgcgact accacgacca gttgatgccg ttctcgctgc tcgagaacta ctacatgatg
     1921 aacggttgct ttctcgacga ccgccagctg ctcgatcacg ccgatcgcat cgccgacatt
     1981 ccgaccttca tcgtccacgg ccgctacgac gcgatctgcc ggcccgtggt cgcctggagc
     2041 ctggcgcagc gactcgacaa cgtcgagttg gtcttcaccc acgccggcca ctcgcagtcc
     2101 gacccagcca acacggacgc cttgctggca ggactggact gggtcgccga ccggatcgag
     2161 gccgacggtc tacgcccgca gatcgcagat cgctgaccgg gctcgcgaga tcatgggacg
     2221 tccgacaggc cttcggcgtg cacgtagcgg aaccagacca gctcgtcgat ctccgcgtcg
     2281 ccggtcggga ggttccaccg cgggcgcgtg accttgaacc gcgcacccaa ccggaccgcc
     2341 agatcgcgtg cctcgtcgaa gtagcgctgc cgctcctcgc gcagcgcggt ttcgccgagc
     2401 cgccgctcgt agacgccgcc gcgcgggttg tcggccgcat cttcgagcgc ctcgatcgac
     2461 agctcgattc cgggggacgg cggcacgacc ccgtcgatgt gacgccacac tccgtcacgc
     2521 cagttcagcc ggtagagcgg gacgaagtcg atgccgtggt cggcgacgaa ctcgatggca
     2581 cgcaacacga actcgatgtc ttccgggttg gcgtagtacg ggagcgacag ccgcacccag
     2641 cccggcttca tcccgttcag gccgcgacgg accagccgcc ggaatcgcgc cgacgcctcg
     2701 ggccggatac caagcaggtc gtggccgtac ggcccggcac aggagcagcc cgcgcggttc
     2761 tggataccga acagatgatc gagcagcgcc gatgcgaggt cgtgatgcag gccgtcgatc
     2821 gacagcgaca ggatcgcgag acgcggcagg tcgcgtggac cgagaacttt gatcttgcga
     2881 tgttgcgcca gcctggcgat cgctgcctcg gccagccgca tctcgtgctc gaggatctcc
     2941 tgcgggccga tcgcgtgttt gacgaggaag ctggcaccgg cccgcaggtc gccggcgatc
     3001 gccggtgttc caccttcctc gcgttcggcg aggccggtga cgtagtcgac cgcgtcgcgt
     3061 ctggcgccgg cgacgtagtc gaccgtgccg ccgcccgggc gctcgggaac gcgcgagcgg
     3121 aacagctccc gccgcgcgac gagcacgccc gagctgcccg gaccgccgag gaacttgtgc
     3181 ggcgacagga acagcgcgtc gacccgctcg tcgtccgtgc ccgggtgcat gtcgatcggc
     3241 acgtagggtg cggccgcggc gtagtcgaag aaggccagcg cgccgtttcg gtggagcacg
     3301 cgagcgatcg cgcgcacgtc gctcaggact ccggtcacgt tcgaggccgc ggagaacgac
     3361 ccgaccttcg tctcgcgcgc ggagtactgc gccagccgcg cctccagctc gcccaggctg
     3421 atcttccccg acgagtccgc gccgatctcg acgacctcgg cgatcgactc gagccacggc
     3481 agcagattcg aatgatgctc gtacggcccg acgaagacga ccggtcgcgc cgccgcgggt
     3541 atcgcctggc cgaggcgatg gacgcgttcg agcggttcgg aaatgcgcca gccgagcagg
     3601 ccgacgagct tgttcgccgc agccgtcgcg cccgagccga cgaacaccac ctcgtcctgg
     3661 ttggacgcgt tgaccgcctg ggcgatgatc gcccgcgccg tggcgcgcag ctcggtcagc
     3721 aggttgcccg tcgacgatac ggcggtgtgg gtattggcgt agtacggcga gagtcgattg
     3781 acccactcgt cgacgaacgc caggtgacga ccggtcgccg tcaggtcagc gtagtagatc
     3841 agccgcgggc cgaacggcgt ctggatccgc gtgtgaccgc cgaccacctg ctctctgagc
     3901 caggtggtga cctcgcggaa ctgttgcgac atcccgacgt ccatcgtgtc ggatccagtc
     3961 tagcaagacg gtccggtcgg gtcaggccgc cggcggacga aaaaccgacc cgaaacggtg
     4021 cttgaaccgc tccgacccga tccccgcttc gccttgcccg cgcccgccgg tctggcgcgt
     4081 ctgctcggtg cgatacctcg atctgcgcga ggccgggttg ctgatcgagc agcggctgcc
     4141 ggagatgttg gtcgacccgg actacgcgtc gcgcccgacc cgggagcgct gcgacgcggg
     4201 tcgagatgca gagcctgttg tcgttcaagg gatgcacgct gatcgactcg tcgttgattc
     4261 gcagcagcgg ggagtcgcag gtctcggtcg gaccgcgctg cagatcgatc ggttccagct
     4321 cgcgtcgccg gtcggcgacc gctgggccag ccacgtcgtt ctcgacggcg gtcggggaga
     4381 ccgtggtcgt cggcacctcg cgcccgatgg agagccacgg ggcgctggtc gtactactta
     4441 ccgcgctcga atgagccggc gctcacgtcg gcgtcggtct gcagcacgtg tctcggccgc
     4501 ggcgcggaag gcgggtgaac cgcaccgaat cgaccgcgtc tcgtcgccac tgccgcctgt
     4561 acgcgggcca gtgagcactc gtcgggcgca cccacccgct tgccgacctc tgaacgcgcg
     4621 gcggtcgaga ccaactcctc gccgtgctcc gcgtcgatcc gcccctcgtc gaccagcacg
     4681 gcgacctcgc gagcgacgca gctcacgtaa tggccgtggt tgcgccagct ctcgcacggg
     4741 tcgcaggaga ccaggccgtc gcagttttcg tccagtcggt tgccgggcag ctcggctgcg
     4801 tcgggatgga ccgcggcatc ttcgtcgtcg cagtcgaccg gcgccgtgaa gccgtctccg
     4861 tcgtcatcgt gcgatttgca cttgccggcc ggtccggctt ggtggatcgc gcggatctcg
     4921 tcgggcgtca gcgcgcgatc gaagatctcg acctcgtcga tccggccgtc gaagacgccg
     4981 cgcggactgt gggaggtgtg gaggatgttg ccgatccgca gcggtgcggg gccggtgtag
     5041 gcggtcacct gcccgatcag cgcgcgccct tcctcgacgg cgtcgacgta cagcagcagg
     5101 ttgatgccgt cgtagaccgc ggcgatgtgg taccagctcg ccgcgggatg ctcggccgac
     5161 gtgacgacga agtcctgccc gtccgacgcg cgttcggtcg aggtctgaaa gcggttgcgc
     5221 ccagcgcagt cgtggcaacc ccaaccgaga tcgaacgtgt cgttgttcgt gttcggcgcg
     5281 ccgatcttcc aaacgatcgg atcgaggcgc gcgtcctggc cgtccgctgc cgggaagacc
     5341 caggcgtcga cggtccaggc ttcgacgaga tcgaacgcgc cgtcggcgtt cgtcacctcg
     5401 atcccggcgt cgatgccgtc gaagacgaac gcctgtccga cgacaccgtc gccgaacccg
     5461 acttcgccga gcagcgctcc gtcgacaccg cgcgccagat cctcccccga cccgtcaccg
     5521 ggccaccagc cgatcagccc ggacggtaca tcgacgcact gctgcgacca cgacgcggtc
     5581 ggagcccagg gcaacgacaa tatcgagcag atcgcgatcc gggccatcgc ggtacggttc
     5641 ggcacggcga cctccgagtg cgaatgggcg aaacccgcgg gcgcccgcag cccgggcaag
     5701 ccgtccctac aacggcgacg ggcgcggggc gagtcggggc cactcgtttc gcaaccgacg
     5761 tgccatcacc gcttccggcc ggatcgagac gatccggcgg atagcggctg cgaaactgtg
     5821 agggcgcgat acgattgtgc aagtccgccg ggcgtcggat ctagtcgccg aggtagcgat
     5881 tcagcctcga ccatccgcga gccatgccga gcagcatgtc cgaccgcatt cgagcgcgaa
     5941 cgcggtcgtc gctcgctcga tcgtcgagcc gccgcagctt ccgctcgagc tcttgcagct
     6001 cggcgacgat ctgacgcgcc tcggcattct cgagaagctc aggatgttcg acgacctgcc
     6061 ggaccagacg cgtcgcgacg tgtggcgtca cccaccgctt tcgtccgcgc atttcggacc
     6121 tcccgtcgac gctgagggag caccgtgctg gtcagatggt acacccagaa tgccacgcgg
     6181 cggccgtccg acccccaaaa aagcaagacg atcggccccg gcgcgtgacg cgggtatgat
     6241 cgcccggtga ccgacgaacg ccgaatcgag tcgattcccg gggtttgcga cgtcgagcgg
     6301 ctcggccgga gcgccggcgc gccgaccctg ctgatcgagc tgccgcacgg ggcgacgcgt
     6361 gggagcgact tcgacgcgct cgcggcgcgg ctgatttcga tgctgcccga ccgcttgcgc
     6421 gagttcttct tcgtcaacac cgacgtcggc tgccacgagg ccgcgcggtt gatcgccgcc
     6481 cggctggtcg atcactttcg tgacggcccg gctgtcgagg tcgtgatcgt gcgtagcctg
     6541 ctgccgcgca cgctggtcga ctgcaaccgg ctgctggccg cgtcggccga cgacgctcgc
     6601 cggatgacac cggccatccc gtcgtacgtc gatcgccgcg aggatcgagt gatgctcgcc
     6661 gacctgtacg cgcggtaccg tcgtctcgtc gagcgcgctt acgaggcgtt ggacgccgac
     6721 cgcgggtggg cgctgcagct gcatacctat gccccgcgat cgatccagat cgatacgatc
     6781 gacggttcga tcgtcgagac gctgcgggag gcatacgcgc cggagaacta cgaccgatgg
     6841 cgatcgcgac cgagcgtcga gctgatcagc gaggaccccg cgggagtgat gctggcgccg
     6901 gcccgactcg tcacggcgat caagcgtcat taccgcgccg cgggtatcga gctggccgag
     6961 aacgaaacct accgcctgca tcacgacacg accggctacg tcgacgcggc gcgccggccg
     7021 gggcgcgtct gttgcgtcga gctgaaccgc gggctgctgg ccgagccgtt cgcgccgttc
     7081 gaagagatgc gggtcggcgc agccaagctg gaacgcctgg ccggacctct cgcgtcggcg
     7141 tatatcgaga tcatcgagga aggagcggga gcatgaacaa gttcttcgtt tcgatcgggg
     7201 cgggactcgc gacgatcctt gccgccgggc ccgcgccggc gcaaaccgtc accgatccgt
     7261 cggtgaatct cgaggtcgag tcggtggtcg agggactgtt cctgccgacg agcatggcgt
     7321 tcgtcggcga cgaggagatc ctcgtgctgc agaagaacga cggcctcgtc cggcacgtcg
     7381 tcggcggggt gctgcagccc ggcagcgtgc tcgacgtcga cgttcacgtg cagagcgagc
     7441 gcggactgct cggcatcgcg accgacccgg acttcatcca cacgccgtac gtctacctgt
     7501 acttcaccga gagctcgacc ggcggcgata cgaacgacgg taactcgatt ccgctgggaa
     7561 accgcgtcta tcgctacagc tggaacggca gcgggctggt cgacccgttg ctggtgctcg
     7621 acctacccgc gacgccggga ccgaaccacg acggcggcat cgtcgttttc ggacccgacg
     7681 acgcgctgta cgcggtcatc ggcgacctca accgtaacgg caagctgcag aactacccgg
     7741 ccgggcccga gccggacgac agcggcgtga tcctccgggt gcaccgctcg ggcgcgacgc
     7801 tgaccgacgg tccgttccgc gacgccggtg atccgggcaa cccgttgaac cgctactggg
     7861 cctacggcgt ccgcaactcg ttcggcatgg cgttcgaccc cgtcacgggc gagctgtgga
     7921 acacggagaa cggcccggcc tcctacgacg aggtcaaccg cgttccaccg ggcttcaaca
     7981 gtggatggga acggatcatg ggccccgacg cgcgggacgc cgacgaccag gatgacctgt
     8041 gggtcgcgcc gggatcggcc taccgcgacc ccgagttctc gtgggccagc acggtcgcac
     8101 cggcggcgat cgccttcgtt gccagccgcc agctcggctg cgagctcgag cacgacgtga
     8161 tcgtcggcga cgacaactgc cagcaactct accggttcga accgaacgcg gcgcgcgacg
     8221 gtctggtctt cgccagcccg gagctggccg atcgcgtcgc cgacaacggc ttgttgcgct
     8281 gcagcagcga acagtccgaa gtgctgttcg gttcgtcgtg gggcacggtc accgacatcg
     8341 aaaacgggcc cgacggctat ctctacatcg tctcgctgac gcagggcagg atcctgcggc
     8401 tggtgccgga tcacgggacg ttcgacgacg ccgattcgga cggcgtggag gatgcctgcg
     8461 actgcgccgc gggcgattcg acggcattcg cagagccggg cgaggtcccg cggattcgcc
     8521 tcggcgcggc gacgccgacg acgcttggct gggacccgca gaccgccacc gccggcaagg
     8581 gaacgaccta tcaggtcgtt tcgggtgatc tcgactcgct gcgcgccgcc ggggggttcg
     8641 ccggcgcgtg cgagctggcg gccggcctcg ccacgcccgc gctcggcgat ccgcgcgccg
     8701 acccggcgcc cggcaccggt gtctactacc tgacgcgggc cggcaacgcc tgcgacgccg
     8761 gcacgttcgg cgacggctcc gcgccggccg acccgcgcga cctgctcgac gcggggactc
     8821 cacccgcgtg cccttagcgc cgcagacgag atcgtgcggt tccttcagca gcagcccgcc
     8881 gacgccgtgc tggaaagctc gaccgcgttg tccccggtga ccttctccca gatcgggacc
     8941 gccgcggtgt cgagcggcga accgtctccc gcctcgccgt gcagcgcgtt gtgcggtcgt
     9001 ttcgtgatgc gaaggaaccc cgcgcgcccg gacctcgaac gcttgcggtg ccgccgatct
     9061 tcgttataag gtctgagcga actcgagcga ggtgacccga tgaaggaact gaccgcggaa
     9121 cagctgaaac agcgtctgga acggggtgat gcgttgtccg tcgtcgacat ccgcgaggcg
     9181 gacgagtacg gcgcatggag tatccacggg agtctcaaca tcccggtgta cgatgcgctg
     9241 cgcgcctcgc gcgacgaggc gttgacgacg agc
//
LOCUS       DASPGN010000613         3961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_817732, whole genome shotgun sequence.
ACCESSION   DASPGN010000613 DASPGN010000000
VERSION     DASPGN010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817732"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2600)
                     /locus_tag="VD788_17825"
     CDS             complement(<1..2600)
                     /locus_tag="VD788_17825"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXV78174.1"
                     /translation="MIGRTLSHFEIIDKIGEGGMGVVYRAEDRDLGRQVALKVLHTEF
                     VTDEERRKRFLREARTAASISHPNIATIHEVGESDGVIYIAMELVEGRTLKKVINEDH
                     PAVDECLRLAIEICEGLGSAHQASIVHRDLKPDNVVIDGSGRVNILDFGLAKLYGAPG
                     EDARDGSGLQTISTSVTRDGRILGTVQYMSPEQARGVELDARSDVFSLGIVLYELVTG
                     QLPFHGETITDTLTSILRDEQPPIALFNPQVPVELERILRRCLEKDPSARYQDASQVL
                     ADLRKLKRMSDSQPVPLVSDTSLRRHRAARLPWRRGATLAAAAVLLVALGATAAAVLR
                     GAGWLPGAAAGPRAMAVLPFANLQERDDPERLGQILQELIITDLSGFDTLKVLSSQRL
                     FDVQKQLGRPTSTTIDRDVATRVAELAGADMMLTGSLSRLGSTWILTCQLIDLADGSV
                     VQSDRIDGADLYAMVDDLTAAVRQNLDVDPAATDVAVTERTSSSLEAYQAYLTGVEAL
                     NELGFVQAIDRFEHAIEVDPSFGRAYYKLAIARWWKGSVENYRAEEHRGTPSDALEKL
                     LGGNVKLSRKDRLLAEAFLELVESRPDRALPLFEKIVESFPDEKEGHYGLGEARFHSA
                     TRREERMAALEPFEAAIDLDPSFSLSFYHVVDLYAQAERYDEGIERVRGFIAQDPENH
                     SWYQDLARLALAKDDQRMFDSVVDEALRRIDSRSEQREFLVGVQRMADDLDRRQALLD
                     RALLIETDAMQQELLIAQGRVAAARDEDDRAEGLLRDALALDPRSMPALNALFEYFDG
                     RLRFDDALSEAKRLSASDPDFPAYVGHWAAAAIKRGDAGEIDAAMRAVERLRERRAGE
                     PSAMLPVGQL"
     gene            complement(2686..3798)
                     /locus_tag="VD788_17830"
     CDS             complement(2686..3798)
                     /locus_tag="VD788_17830"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HXV78175.1"
                     /translation="MRPLAGVLVVDASRMLPGAVLGRQLIELGARLIKVEQPLFGDPL
                     RSTPPIVDGCGAGFREFYAGAESIRLDLRRSDDAARLIRLASRADVLVESFRPASSEA
                     WGIAPRRLAALNPRLVVCTLSAFGGTGDNARRVGHDLNFVAGAGALDLMGRTELPRLQ
                     LADVSSGLLACSAILAALLARVASGCGTVLDQPLAAGPLPFLTWALADRAAGGGGLNE
                     PAGLMSGAAPAYRLYSAADGLRVAVCALEPKFWVGLLELLGLPGFADAALDTGRRGLE
                     AAARIAERLAREPRATWLERCRARGLPVTAVNDLDDARHDAAFVAAGLLGMEGCGPYW
                     PAFGPRAPRVAPSLGQHDGSLRAEFGLDPSQSAGPA"
BASE COUNT          620 a         1394 c         1358 g          589 t
ORIGIN      
        1 atcaattgac cgacgggaag catcgccgac ggctcgccgg cacgacgctc ccggagccgc
       61 tcgacggcgc gcatcgccgc atcgatctcg ccggcgtcgc cacgcttgat cgctgccgcc
      121 gcccagtgcc cgacgtaggc cggaaagtcc gggtcgctgg ccgacagccg cttggcctcg
      181 ctcagcgcat cgtcgaagcg cagccggccg tcgaagtact cgaacagtgc gttcagcgcc
      241 ggcatcgacc gcgggtcgag cgcgagcgcg tcgcgcaaca gcccttcggc gcggtcatcc
      301 tcgtcgcgag ccgccgcgac ccggccctgg gcgatcagca gctcctgctg catcgcgtcg
      361 gtctcgatca agagcgcccg atcgagcaac gcctggcgac gatcgaggtc gtccgccatc
      421 cgctgcacgc cgaccagaaa ctccctttgc tccgagcgcg agtcgatgcg ccgcagcgct
      481 tcgtcgacga ccgagtcgaa catccgctgg tcgtctttgg ccaaagccag acgcgccagg
      541 tcctgatacc acgagtggtt ttccggatct tgcgcgatga acccgcgcac gcgctcgatc
      601 ccctcgtcgt accgctcggc ttgcgcgtac aggtcgacga cgtggtagaa cgacaggctg
      661 aacgacgggt cgaggtcgat cgcggcctcg aatggctcga gcgcggccat ccgctcctcg
      721 cgccgcgtgg ccgaatggaa gcgcgcctcg cccaggccgt agtgcccttc cttttcgtcc
      781 gggaaggact cgacgatctt ctcgaacaag gggagcgctc ggtcgggacg cgattcgacc
      841 agctcgagga acgcctcggc cagcagccgg tctttgcggg acagcttgac gttgccgccg
      901 agcagcttct cgagcgcgtc gctcggcgtc ccgcggtgct cttcggcgcg gtagttctcg
      961 acgctgcctt tccaccagcg cgcgatggcg agcttgtagt aggccctgcc gaacgacggg
     1021 tcgacctcga tcgcgtgctc gaaccggtcg atcgcctgca cgaagcccag ctcgttcagt
     1081 gcctcgaccc cggtcagata ggcctggtag gcctcgagcg acgagctggt gcgctcggtc
     1141 accgcgacgt cggtcgccgc gggatcgacg tcgaggttct ggcggacggc ggccgtcaga
     1201 tcgtcgacca tcgcgtacag gtcggcgccg tcgatgcggt cggactgcac gacgctgccg
     1261 tcggccagat cgatcaactg gcaggtgagg atccaggtcg agcccagccg gctgagcgac
     1321 ccggtcagca tcatgtcggc accggcgagc tcggcgaccc gggtcgcgac gtcccggtcg
     1381 atcgtcgtcg acgtcggccg acccagttgc ttctgaacgt cgaacagtcg ctggctgctc
     1441 agcaccttga gcgtgtcgaa gccggacagg tcggtgatga tcagctcctg gaggatctgc
     1501 cccagccgct ccgggtcgtc gcgctcctgc aggttggcga acggcaacac cgccatcgcc
     1561 cgcggacccg cggccgcacc gggcaaccag ccggcgccgc gcagcaccgc ggctgcggtg
     1621 gcaccgagcg cgacgagcag tacggccgcg gcggcgagcg tcgcgccgcg ccgccacgga
     1681 agtctggccg cgcgatggcg tcgcagcgag gtgtcgctga ccagtggcac gggttgcgaa
     1741 tcggacatgc gcttcagctt gcgcaggtcg gccagcacct gactcgcgtc ttggtaacgc
     1801 gcgctcgggt ccttctcgag acagcgacgc aggatgcgtt cgagctcgac cggcacctgc
     1861 gggttgaaca gcgcgatcgg cggttgttcg tcgcgcagaa tcgacgtcag cgtgtcggtg
     1921 atcgtctcgc cgtgaaacgg cagttgcccg gtgaccagct cgtacagcac gatacccaag
     1981 gagaagacgt ccgagcgcgc gtcgagctcg acaccgcgcg cctgttccgg cgacatgtac
     2041 tgcacggtgc cgaggattcg cccgtcgcgc gtgaccgagg tcgagatcgt ctgcagtccg
     2101 ctgccgtcac gagcgtcttc gcccggcgcg ccgtacagct tggccagacc gaaatcgagg
     2161 atgttgacac gcccggaacc gtcgatcacg acgttgtcgg gtttcaggtc gcgatggacg
     2221 atcgaggctt gatgggcgct ccccaaccct tcgcagatct cgatcgccaa ccgcaggcat
     2281 tcgtcgacgg ccgggtggtc ctcgttgatc accttcttga gtgtccggcc ctcgaccagc
     2341 tccatcgcga tgtagatcac gccgtcggac tcgccgacct cgtggatcgt cgcgatattg
     2401 gggtgcgaga tcgaggccgc ggtgcgcgcc tcccgcagga agcgcttgcg tcgctcctcg
     2461 tcggtgacga attcggtgtg gagcaccttc agcgcgactt gacgaccgag gtcgcgatct
     2521 tcggcccggt agaccacacc catcccgccc tcgccgatct tgtcgatgat ctcgaagtgg
     2581 gaaagtgtcc ggccaatcat cgggtcctcg accgcgcggg ctggaagaga ccgtatacct
     2641 tataacgccc gggagccacg aaacgtctcc ccgttttaca agagtttacg ccggaccggc
     2701 cgattgcgac gggtcgagcc cgaactcggc ccgcaacgac ccgtcgtgct ggccgagcga
     2761 cggtgccacg cgcggggcgc gtgggccgaa ggcgggccag tagggcccgc acccctccat
     2821 ccccagcagg ccggcggcga cgaacgccgc gtcgtggcgg gcgtcgtcca ggtcgttgac
     2881 cgcggtgacg ggcagtccgc gcgcgcggca gcgctcgagc caggtcgccc gcggctcgcg
     2941 ggcaaggcgt tcggcgatgc gcgctgccgc ctcgaggccg cgccggccgg tgtcgagggc
     3001 ggcgtccgcg aaacccggca gcccgagcag ctcgagcaag ccgacccaga acttgggctc
     3061 gagcgcgcag acggcgacgc gcaacccgtc ggcggcggag tagagccggt aggccggtgc
     3121 cgctcccgac atcaggcccg cgggctcgtt caacccaccg ccgcccgcgg cgcggtcggc
     3181 gagcgcccag gtcaggaacg gcaacggtcc ggcggcgagc ggttggtcga gcaccgtgcc
     3241 gcaaccgctc gcgacccggg ccaacaaggc ggcgaggatc gccgaacagg cgagcagacc
     3301 gctcgacacg tcggccagct ggagccgcgg cagctcggtg cggcccatga ggtcgagcgc
     3361 gccggcgccg gcgacgaaat tcaggtcgtg cccgacccgc cgagcgttgt caccggtgcc
     3421 gccgaacgcc gacagcgtgc agacgacgag ccgcggattg agcgccgcga gacgccgcgg
     3481 ggcgatgccc cacgcctcgc tgctcgccgg gcggaagctc tcgaccagga cgtcggcgcg
     3541 cgatgccagc cggatcaggc gggcagcgtc gtccgaacgc cgcaggtcga gccggatcga
     3601 ctcggcgccg gcgtagaact cgcggaagcc cgcgccgcag ccgtcgacga tcggtggcgt
     3661 cgatcgcaac gggtcgccga acaggggttg ctcgaccttg atcaaacgcg cgccgagttc
     3721 gatcagctgg cgtccgagca ccgccccggg cagcatgcgg ctggcgtcga ccaccagcac
     3781 gccggccagt ggtctcaccg gccgtcccac aacagcttgg ccaggcccat catcagcagc
     3841 aggttgctga cctcgatctc gccggtcagg aacgccgcct gcgggctgag cgagccgtca
     3901 cggattccga gcagggtgcg ctccggcatc cggatcgtgc agtccgagct gccttccagg
     3961 c
//
LOCUS       DASPGN010000614         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_114904, whole genome shotgun sequence.
ACCESSION   DASPGN010000614 DASPGN010000000
VERSION     DASPGN010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114904"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VD788_17835"
     CDS             <1..1057
                     /locus_tag="VD788_17835"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV78176.1"
                     /translation="RLMATIGTVYKKMGLYDDALPLLERSTAIHRAKLGDADPATLRS
                     KLELAELLFFQGRLAEAEAMLRRTHEQSRVRLGEDHPQTIAAQADLAVVVHFAGRLDE
                     AERLYLIALEARRRVLGEDHAETLETTNNLAAMFAAQGELSAAEPYLRAALEGRERAL
                     GPEHPETIDSMINLGTVLQALGRYDEAEVHCRRAVEIGRRVRADHPKTLVSINNLAKL
                     LELRGDHAQAERYYHEALAALERSLGRGHADTLTAMGNLGDLYTSMRRYDEAEQLLSS
                     AVREVRRSLGDSHVVTGYTLTKYGRLLGATGRQAEAERILTDARSVLTRAVGPDHPRT
                     VKAEELLAEVERARLAE"
     gene            1101..1964
                     /locus_tag="VD788_17840"
     CDS             1101..1964
                     /locus_tag="VD788_17840"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXV78177.1"
                     /translation="MTSCFFASDLHGSIPRYEKLWRAIEVDRPAAVFLGGDLLPSGLT
                     GLASSAPAHLDFVNDVMRAGFDRVRDRLGRRYPKVFLVLGNDDGRFEEAAILDAAASE
                     VWVYAHNRSLSWSGYRIFGYNFVPPTPFRWKDWERYDVSRHVPPGCISPEDGRHSLPT
                     APDEIKYRTIGTDLESLVADFDLTNALMLFHTPPHETKLDRAANDGKMVDWVPLDLHV
                     GSIAVRRLIESRQPLVTLHGHIHESARLTGSWRDRIGRTHLFGGAHDGDELALVRFDA
                     EQPSNAIRQLL"
BASE COUNT          343 a          702 c          733 g          335 t
ORIGIN      
        1 ccgactgatg gcgacgatcg gcaccgtcta caagaaaatg ggactgtacg acgacgcgct
       61 gccgctgctc gagcgctcga cggcgatcca ccgagcgaag ctgggcgacg ccgatccggc
      121 gacgctgcgc tcgaagctcg agctggcgga gctgctgttc ttccaggggc ggctggccga
      181 ggccgaggcg atgttgcgtc gcacccacga gcagtcccgc gtgagactgg gcgaagacca
      241 cccgcagacg atcgctgcgc aggccgacct ggccgtggtc gttcatttcg cgggacgtct
      301 cgacgaagcg gagcggttgt acctgatcgc gctcgaggcg cgccgacgag tgctcggcga
      361 agaccatgcg gagacgctcg agacgaccaa caacctggcc gcgatgttcg ccgcccaggg
      421 cgagctgagc gcggccgagc cctacttgcg cgcggcgctc gagggtcgcg agcgtgcgtt
      481 ggggccggaa caccccgaga cgatcgattc gatgatcaac ctgggtacgg tgctgcaggc
      541 gctcgggcgc tacgacgaag ccgaggtgca ctgccgccgg gcggtcgaga tcggccgccg
      601 cgtccgtgcc gatcacccga agacgctggt gtcgatcaac aatctggcca agttgctcga
      661 gttgcgcggc gaccacgcgc aggccgagcg ctactaccac gaggcgctcg cggcgttgga
      721 gcggagcctc ggccgtggtc acgccgacac gctgacggcg atgggcaacc tgggcgatct
      781 ctacacgagc atgcgtcgct acgacgaggc ggagcagctc ctgtcgtccg cggtgcggga
      841 ggtccggcgg tcgctcggcg attcgcacgt cgtgaccggc tacacgctga ccaagtatgg
      901 ccggttgctc ggcgcgaccg ggcgtcaagc cgaagccgaa cggatcctga ccgacgcgcg
      961 atcggtcttg actcgagcgg tcggtcccga ccatccacgg acggtcaagg ccgaggaact
     1021 gctggccgag gtcgagcgag cccggctcgc ggaatgaccg actcgacgac cgattccgcc
     1081 gtgcgatact gtgcgcggcg atgacgtcct gctttttcgc gtcggacctg cacggcagca
     1141 tcccgcgata cgagaaactg tggcgcgcga tcgaagtgga tcggccggcc gccgtgttcc
     1201 tcggcggcga tcttctccct tcgggtctga ccgggctggc ctcgtcggcg ccggcgcatc
     1261 tcgacttcgt caacgacgtg atgcgcgccg ggttcgaccg cgtccgcgac cggttgggtc
     1321 gacgctaccc gaaggtgttc ctcgtgctcg gcaacgacga cgggcggttc gaggaagcgg
     1381 cgattctcga cgccgccgcc agcgaggtat gggtctacgc ccacaatcgc agcttgtcgt
     1441 ggtcgggata ccgcatcttc ggctacaact tcgtcccgcc gacgccgttt cgctggaagg
     1501 actgggaacg gtacgacgtc tcgcgccacg tcccgccggg gtgtatctcg ccggaggacg
     1561 ggagacattc gctgccgacc gcgcccgacg agatcaagta caggacgatc gggaccgatc
     1621 tggagtcgct cgtcgccgat ttcgacctga ccaatgcgct gatgctgttc cacacgccgc
     1681 cgcacgaaac gaagctcgat cgtgcggcca acgacgggaa gatggtcgac tgggtcccgc
     1741 tcgatcttca cgtcggcagc atcgccgtgc ggcgactgat cgagtcacgc cagccgctgg
     1801 tgacgctcca tggccacatc cacgagtcgg cccgcctgac gggcagctgg cgcgaccgga
     1861 tcggccgcac ccatctgttc ggtggggcgc acgacggcga cgagttggcg ctcgttcgct
     1921 tcgacgcgga acagccgtcg aacgcgatac gacagctgct gtagatcggc tcggcggcga
     1981 cggtcagcgg cggcccagcc gcaactcccg cgccgcgtcg gtcaccgcgt cgatcttgac
     2041 cgcgtagctc gtccgttgcg cgatgtcgtc atcggtgacg tggtgcacgt cgagcagccc
     2101 gtcgctcgag tag
//
LOCUS       DASPGN010000615         5980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1637890, whole genome shotgun sequence.
ACCESSION   DASPGN010000615 DASPGN010000000
VERSION     DASPGN010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1637890"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VD788_17845"
     CDS             <1..442
                     /locus_tag="VD788_17845"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXV78178.1"
                     /translation="LVLTACRESGDVVHGLDSGADDYLTKPFSMNELCARARALVRRA
                     HDRPDPMLVAGRIRVDTLRHVVICDGREWRLPAMEYRLLEYLALRAGQVVSKTELFER
                     LYDFNSDRLSNVIEVYVSSLRRRFGAGSIQTVRGHGYALTGDGP"
     gene            439..1836
                     /locus_tag="VD788_17850"
     CDS             439..1836
                     /locus_tag="VD788_17850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXV78179.1"
                     /translation="MSRWSLAGRLRLLVALGTAVVLVVVVVTAYRTFRAAYVGQLDRI
                     LQVMATGVASVAARAATATGLDGEVQAIVASPWRGPGNEVRVWLDGESRDLVAGVASA
                     RRELIGSDPSTFPPPAIGEARYFDLDHKQRSFRAVWLRRQADRGVINVLILHPANYEQ
                     RRLRSLATTLAATSGLVLLLAIGLATPLARLAFRPLRPLVERLGAITPTAPRALLDGA
                     PGAPVELTPLVRAIDELVARFSATIERQRALVAEASHQLRTPLAIARSTLEVSEGDAP
                     SGQVSRELLEELTRMEHLVEQLVLLARVETGPLSDPVEQLRLDAVLEELAASYRAKAD
                     THGIRIDCRQLAPCEIDGDEVLLRALFGALLDNAMQYGPAGGTVRVTLEQGPAGQCTA
                     TVQDDGGGIPAVALACLFDRFYRDDSTRAQNPAGAGLGLSIAREIARRHGGEVWATSV
                     PGRRTSFHVRLPMRG"
     gene            complement(1850..2689)
                     /locus_tag="VD788_17855"
     CDS             complement(1850..2689)
                     /locus_tag="VD788_17855"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXV78180.1"
                     /translation="MVELQRTGVVWVGAVASLVALGCSGGAQGEIVGAPMNLSVGPSA
                     SDDFASIGEAIAAATPDSTIVVATGTYTEALFVDKALTIVGSGVGTVVEFPANGPADS
                     AVIEIRDARGVRIENLLVRASRLDVDGIRVRDSTDVVLDSVVAEGNSQDGVDVKRSAG
                     VTLHMARFTDNGADGLQIDESSSDVTVTDCRAESNQVDGFKIRASSNVVLEGNTSTLN
                     LDDGILVRDSSAVRLTGNTVTNNGDWGITVQNSPDTALENNAASGNGDGQIRCEPTAC
                     AGP"
     gene            2734..3537
                     /locus_tag="VD788_17860"
     CDS             2734..3537
                     /locus_tag="VD788_17860"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HXV78181.1"
                     /translation="MKPLPQPPPLPHEHGAWVMLVLPLLLGQAAAGGRSPAGWSFSAA
                     VIAVFLAHYALVPIVERRLAGRNAPADWSRRRRVWGAIYFGVALALFAATVGLAPAAN
                     RSVITGLATTAAVLGAVYFTAACLGAGRRAAVELVGMVAMSVSAPIVAFASGRPFSAR
                     PLGAAVLAVVYSASALSFVRAFGAARERRGRALVACLAQHAALVAIIAWVARDGWLPS
                     WWWISFVPLVARVVWGFVLPPPNLRRLGLREIWVALTYAVLAVALVVAR"
     gene            3570..4088
                     /locus_tag="VD788_17865"
     CDS             3570..4088
                     /locus_tag="VD788_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78182.1"
                     /translation="MGGRGFHWLLIALCTMLAIVVILLARQNAELKRELLARPAVELS
                     AGDVLDEIVVVAHDGVERPLELTGPTLLLVFSSRCPACAALRPYWSELPALAGPGALQ
                     IIGLETDAAGSTALAPYPIYAVVHDRSPGLEPVPYVPATVLVDGDKTVRRVWFGPLDA
                     EARRELTELIRG"
     gene            4182..4406
                     /locus_tag="VD788_17870"
     CDS             4182..4406
                     /locus_tag="VD788_17870"
                     /inference="COORDINATES: protein motif:HMM:NF012280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kazal-type serine protease inhibitor"
                     /protein_id="HXV78183.1"
                     /translation="MKMLKRFVAAGFVAALLVSAAVGVMTLVSSPAEAGRCLCPKIYA
                     PVVCDNGKTYANQCLADCRNAEGCVPTGGI"
     gene            complement(4403..5689)
                     /locus_tag="VD788_17875"
     CDS             complement(4403..5689)
                     /locus_tag="VD788_17875"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV78184.1"
                     /translation="MGRSAAILCCAAALAVAPPLPADDWPQWRGPARDGRSAERGLLE
                     SWPAGGPPLLWRAVGFGSGYSSVAVAGGKVFTLGDVGEDQFLIAASDLDGSLLWKQRV
                     GPAWSEAMFGGSRSTPTVDGGRVYALGTDGDLLCADAETGEALWRRDLVEEFAGKMMF
                     AGGRYDWRFSESPLVDGDRVVVSPGARDAALVALDKKTGEPIWRAAIPPLGEQGLDGA
                     AYASAVVSQGAGVRQYVQLLGRGLVGIDAATGRFLWGYNRIANSVANIPTPIVTGDFV
                     FASSGYGAGAVLLKLDKREDGVRAREVYFLDGATLQNHHGGLVLDDGYVYGGTGLNKG
                     FPLCIELATGKVAWGPIRNLGRNSVAVIYADERLYLRYQSGLMLLVEATPAGYREHGS
                     FTIPDVQKESWSHPVVANGRLYLREQDQILVYAIRR"
     gene            complement(5733..>5980)
                     /locus_tag="VD788_17880"
     CDS             complement(5733..>5980)
                     /locus_tag="VD788_17880"
                     /inference="COORDINATES: protein motif:HMM:NF018652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78185.1"
                     /translation="ERLVRELVLQMKLGRVDLGALDQRYGRAMTARFDEPLGRLADEG
                     LLAIDGRRVRLTYDGLARVDHLLPSFYLPQHRDIRYS"
BASE COUNT          770 a         2158 c         2132 g          920 t
ORIGIN      
        1 gctggtgctg acggcctgcc gcgaatcggg agacgtcgtc cacggcctcg actccggcgc
       61 cgacgactac ttgaccaagc cgttctcgat gaacgagctg tgcgccaggg cgcgcgcgct
      121 ggtgcggcgc gcccacgacc ggccggaccc gatgctggtc gcggggcgga tccgcgtcga
      181 cacgctgcgg cacgtcgtga tctgcgacgg ccgcgagtgg cgcctgcccg cgatggaata
      241 ccgcctgctc gagtacctgg cgttgcgcgc cgggcaggtc gtgtcgaaga ccgagctgtt
      301 cgagcgcctc tacgacttca attcggatcg gctgtcgaac gtgatcgagg tctacgtctc
      361 gtcgctgcgc cggcggttcg gcgcggggtc gatccagacc gtccgcggac acggctacgc
      421 gctgacgggc gacggcccgt gagtcgctgg tcgctcgccg gacgcctgcg cttgctggtc
      481 gcgctgggca cggccgtcgt cctcgtcgtc gtcgtcgtga cggcctaccg caccttccgc
      541 gcggcctacg tcggacagct cgatcgcatc ctgcaggtga tggcgacggg cgtggcctcg
      601 gtcgccgctc gagcggcgac cgcgaccggg ctcgacggcg aggttcaagc gatcgtcgcc
      661 agtccgtggc gcggcccggg caacgaagtc cgcgtctggc tcgacgggga aagccgggac
      721 ctggtcgccg gcgtcgcgtc ggcgcggcgg gagctgatcg ggagcgaccc gtcgaccttc
      781 ccgccgccgg cgatcggcga ggcgcgctac ttcgacctcg accacaagca acgatcgttc
      841 cgcgcggtct ggttgcgacg gcaggccgac cgcggcgtga tcaacgtgct gatcctccat
      901 ccggccaact acgagcagcg tcgcctgcga tcgctcgcga cgacgctggc cgcgacgagc
      961 ggtctcgtcc tgctgctcgc catcgggctg gcaacgccgc tggcgcggtt ggcgttccgc
     1021 ccgctccggc cgctcgtcga gcggctgggc gcgatcacgc cgaccgcgcc gcgtgcgctg
     1081 ctcgacgggg cgcccggcgc accggtcgag ctgacgccgc tcgtccgcgc gatcgatgag
     1141 ctggtcgcgc gcttctcggc gacgatcgaa cggcagcgcg cgctcgtcgc cgaggcgtcc
     1201 caccagctcc gcacgccgct cgcgatcgcg cgcagcacgc tcgaggtgtc ggagggggac
     1261 gccccgtccg gccaggtgag ccgcgagttg ctcgaggagc tcacccgcat ggagcacctc
     1321 gtcgagcagc tcgtcctgct cgcccgcgtc gagaccggcc cgctctccga tccggtcgag
     1381 cagctaaggc tcgacgccgt gctcgaggag ctggccgcat cgtacagagc gaaggccgac
     1441 acgcacggga tccgtatcga ctgccggcag ctcgccccgt gcgagatcga cggcgacgag
     1501 gtgctgcttc gcgcgctgtt cggcgcgttg ctcgacaacg cgatgcagta cgggcccgcg
     1561 ggcggaacgg tgcgcgtcac gctcgagcag ggcccggccg gccagtgcac ggcgaccgtg
     1621 caggacgacg gcggcggcat cccggccgtt gcgctcgcgt gcctgttcga tcgtttctac
     1681 cgcgacgact cgacgcgcgc gcagaacccg gcgggagcgg gtctcgggct gtcgatcgcg
     1741 cgggagatcg cgcgccgcca cggcggcgag gtgtgggcca cctcggtgcc cggccgccgg
     1801 acgtcgttcc acgtgcggct gccgatgcgt ggctgaccgc cggcccccct cacgggccgg
     1861 cacaggcggt cggttcgcag cggatctggc cgtccccgtt gccgctcgcc gcgttgttct
     1921 cgagggccgt atcgggcgag ttctggacgg tgatgcccca atcgccgttg ttcgtcaccg
     1981 tgttgccggt caaccggacg gcggaggagt cgcgcacgag gatgccgtcg tcgaggttga
     2041 gcgtcgaggt attgccctcg agcacgacgt tcgacgaggc acggatcttg aagccgtcga
     2101 cctggttcga ttcggcccgg cagtcggtga ccgtgacgtc ggacgagctc tcgtcgatct
     2161 gcaggccgtc cgcgccgttg tcggtgaagc gagccatgtg cagtgtgaca cccgcggacc
     2221 gtttgacgtc gacgccgtcc tgcgagttgc cctcggcgac gaccgagtcg aggacgacgt
     2281 cggtcgagtc gcgaacacgg atgccgtcga cgtcgagccg tgacgcgcgc accagcagat
     2341 tctcgatacg cacgccgcga gcgtcgcgga tctcgatcac cgcggagtcg gccggaccgt
     2401 tggccgggaa ctcgacgacg gtgccgaccc cggagccgac gatcgtcagc gccttgtcga
     2461 cgaagagcgc ctcggtgtag gtcccggtcg cgacgacgat cgtgctgtcg ggggtcgccg
     2521 cggcgatcgc ctcgccgatc gaggcgaagt cgtcggaggc tgacggcccg accgacaggt
     2581 tcatcggtgc gccgacgatc tcgccctgcg cgccgcccga gcagccgagc gcgaccagcg
     2641 aagcgaccgc accgacccag acgacgcccg ttcgctgtag ctcgaccatc acctacctcg
     2701 gctagaatct accgccggca agctgaagtg cacgtgaagc ccctacccca acctcctccg
     2761 ctgccccacg agcacggcgc gtgggtcatg ctcgtgctgc cgctgctgct gggtcaggcg
     2821 gcggcgggcg gccggtcgcc ggccggctgg tcgttctcgg ccgcggtgat cgcggtcttc
     2881 ctcgcgcact acgcgctggt gccgatcgtc gagcggcgcc tggccgggcg aaacgcaccg
     2941 gccgactggt cgcggcgccg gcgggtctgg ggcgcgatct acttcggtgt cgccctcgcg
     3001 ctgttcgccg cgaccgtcgg cctcgcgccc gcggccaacc ggtccgtcat caccggtctc
     3061 gcgacgaccg ccgcagtgct cggtgccgtc tacttcaccg cggcctgtct cggcgcgggc
     3121 cggcgcgccg ccgtcgagct ggtcggcatg gtcgcaatgt cggtttccgc cccgatcgtg
     3181 gcgttcgcct cggggcggcc gttctccgca cggccgctcg gcgccgccgt gctggccgtc
     3241 gtctactccg cgtcggcgct gtcgttcgtg cgcgcgttcg gcgccgcgcg cgagcgccgc
     3301 ggccgggcgc tcgtcgcctg cctggcccaa catgcggcgt tggtcgcgat catcgcgtgg
     3361 gtcgcgcgcg acggttggct gccgtcgtgg tggtggatct cgttcgtgcc gctcgtcgcc
     3421 cgcgtcgtct gggggttcgt cctcccgccg ccgaacctcc gccggctggg gctgcgcgag
     3481 atctgggtcg cgctgaccta cgccgtgctg gccgtcgcgc tcgtcgttgc ccgataatgg
     3541 tgcgccggac gggggcagga ggtcggggcg tgggaggtcg cggattccac tggctgctga
     3601 tcgcgctgtg cacgatgctg gcgatcgtcg tgatcctgct ggcgcggcag aacgccgagt
     3661 tgaaacgaga gctgctggcg cgacccgcgg tcgagctgag cgcgggcgac gtgctcgacg
     3721 agatcgtggt cgtcgcgcac gacggcgtgg agcgaccgct cgagctgacc ggcccgacgc
     3781 tgctgctcgt cttcagctcg cgctgccccg cctgtgcggc gctgcgtccg tactggagcg
     3841 agctgcccgc gctcgccggc cccggtgcgc tgcagatcat cggcctcgaa accgacgccg
     3901 caggctcgac ggccctcgcg ccgtacccga tctacgccgt cgtgcacgac cggtccccgg
     3961 gtctcgagcc cgtgccgtac gtgccggcga cggtgctggt cgacggcgac aagaccgtcc
     4021 gccgcgtctg gttcggcccg ctcgacgcgg aggcccggcg cgagctgacc gagctgatcc
     4081 gcggttgacc gggtggttcc ggccggccgg ctctcgcggt atactccgtt cactttccgg
     4141 ccggtgccat gcgggcgccg gcgcgaacaa ggggggttct aatgaagatg ctcaagaggt
     4201 tcgtcgccgc cggcttcgtg gccgcgctgc tcgtgtccgc cgcggtgggc gtgatgacgc
     4261 tcgtgtcgtc gccggccgag gccggacgct gtctgtgccc gaagatctac gccccggtcg
     4321 tttgcgacaa cggcaagacc tacgccaacc agtgtctggc cgactgcagg aatgccgaag
     4381 gctgcgtgcc gaccgggggc atctaacgcc ggatggcgta gaccaggatc tggtcttgct
     4441 cccgcaagta gagccggccg ttcgccacca ccgggtgcga ccagctctcc ttctgcacgt
     4501 ccggaatcgt gaacgagccg tgctcgcggt agcccgcggg cgtcgcttcg accagcagca
     4561 tcaatccgct ctggtagcgt aggtagagcc gctcgtcggc gtagatcacc gcgacggagt
     4621 tgcgccccag gttgcggatc ggcccccagg cgaccttgcc ggtcgccagc tcgatgcaca
     4681 gcggaaaccc cttgttcaag ccggtgccgc cgtagacgta cccgtcgtcg agcaccagac
     4741 cgccgtggtg gttctgcagc gtggcgccat cgaggaagta gacttcgcgg gcgcggacgc
     4801 cgtcttcgcg cttgtcgagc ttcaacagca cggcacccgc gccgtagccc gacgaggcga
     4861 agacgaagtc gccggtcacg atcggcgtcg ggatgttggc caccgagttg gcgatgcggt
     4921 tgtagcccca gaggaagcgg ccggtcgccg cgtcgatgcc gacgagccct cgcccgagca
     4981 gctgcacgta ctgtcgcacg ccggcgccct ggctgaccac ggccgacgcg tacgccgcgc
     5041 cgtcgagccc ctgctcgccg agcggcggga tcgcggcgcg ccagatcggc tcgcccgtct
     5101 tcttgtcgag cgcgacgagc gcggcgtcgc gcgcgcccgg gctgaccacc acacggtcgc
     5161 cgtcgaccag cggcgattcg ctgaagcgcc aatcgtagcg cccgccggcg aacatcatct
     5221 tgccggcaaa ctcctcgacc aagtcgcgcc gccacagtgc ctcgcccgtt tcggcgtcgg
     5281 cgcacagcag gtcgccgtcg gtgccgagcg cgtagacgcg cccgccgtcg acggtcggcg
     5341 tcgagcgcga cccgccgaac atcgcctcgc tccaagccgg cccgacccgc tgcttccaca
     5401 acagcgagcc gtcgaggtcg gatgccgcga tcagaaactg gtcttcgccg acgtcgccca
     5461 gcgtgaacac cttgccgccg gcgacggcga cgctcgagta gccgctgccg aacccgaccg
     5521 cgcgccacag cagcggcggg ccgccggcgg gccagctctc gagcaagccg cgctcggccg
     5581 atcgcccgtc gcgcgccggg ccgcgccact gcggccagtc gtccgccggc agcggtggcg
     5641 cgacggccag cgccgcggca cagcacagga tcgcggcgga gcgtcccatc ggtcgacctc
     5701 cctcgtggac gatcgcgcga tcgtccggcg gctcaactgt aacggatgtc gcgatgctgc
     5761 ggcaggtaga acgacggcag caggtgatcg acccgtgcca gaccgtcgta ggtcaacctg
     5821 acccggcggc cgtcgatcgc gagcagcccc tcgtccgcca gccggccgag cggctcgtcg
     5881 aatcgcgcgg tcatcgcgcg gccgtagcgc tggtcgagtg ccccgaggtc gacgcggccg
     5941 agcttcatct gcagcacgag ctcgcgcacg agccgttcct
//
LOCUS       DASPGN010000616         4884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1401578, whole genome shotgun sequence.
ACCESSION   DASPGN010000616 DASPGN010000000
VERSION     DASPGN010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1401578"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /gene="ssb"
                     /locus_tag="VD788_17885"
     CDS             complement(<1..331)
                     /gene="ssb"
                     /locus_tag="VD788_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXV78186.1"
                     /translation="MGVNKVILVGNLGRDPEVRYTQGGTPVANFPIATSERWYDQASG
                     ERKERTEWHRLVVFGKQAEIAGEYLRKGRQIFVEGSLQTREWTDRDGNKRYTTEVRVQ
                     NFQMLGSR"
     gene            528..974
                     /gene="dtd"
                     /locus_tag="VD788_17890"
     CDS             528..974
                     /gene="dtd"
                     /locus_tag="VD788_17890"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HXV78187.1"
                     /translation="MQRVSRAAVQVDGRTVGRIGRGLLVLLGVERGDEEVDVVYMADK
                     TAELRIFPDEDGKMNRSVEETAGAVLVVSQFTLAASTRKGRRPSYVGAADPTDAEAHY
                     ERFIARLRDRGLVVESGRFGAQMQVELVNDGPVTILLDPRSTGAEA"
     gene            971..1891
                     /gene="xerD"
                     /locus_tag="VD788_17895"
     CDS             971..1891
                     /gene="xerD"
                     /locus_tag="VD788_17895"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HXV78188.1"
                     /translation="MTRKRVTRAEPRAPGVWVERYLDYLTVERGLSPNTLAAYRADLT
                     RLRGFLPREMALERAGRDDLRRFLHQMRADGRSPRSVARWVVAVKGFFGYLVAEGALA
                     VDPAAQLDPPRIWRSLPKVLTFEEVESLLAGPDRSEPRGLRDAAMMEVLYATGLRVSE
                     LLRLHLGDLHLDAGYLRCWGKGAKERVVPLGGEADGTLQRYLAEARPVMLGQRRSDSL
                     FVNARGTPLTRQGFWKMIKRYGIQAGITTPLSPHVVRHSFATHLLENGADLRAVQVML
                     GHADISTTQIYTHVNRERLRRLYEDFHPRA"
     gene            1906..3216
                     /locus_tag="VD788_17900"
     CDS             1906..3216
                     /locus_tag="VD788_17900"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78189.1"
                     /translation="MTIRGATPARIDRRRLIRGLGRRRAALLFGVGSLAADLAADAYL
                     VGGGVRDLLLDRRTTDLDLAIEGDAREVGRRLARVESGEYREHASFGTASVTLADGVR
                     VDLAGTRCERYPRPGELPRVAPSGLLDDLARRDFTVNSMALSLRPERFGRLVDPYDGA
                     DDLRRGRLRVLHSRSFEDDPTRVARAMRLAARLGFRVEPATRRWMDACLGSGALARLS
                     AARRRREVELLFAESGWRAIARTLHRHRAWSVIDRAVQPPPAAAAQLARIEHWYDWLT
                     ALAGFDPPPRWIAALVRLTRDGTSSVRRDVVQRLAPDRHARAMLLSASVEARALLARL
                     RSVREPHPSTVLGAAAGISEAAWLEALDRTRAGRTRGALVDFLRHGRGVRLDISGHDL
                     ERAGIPRGPRIAVGLAAALAAKIDGNAADRHRELEVALVAARRA"
     gene            3239..4222
                     /locus_tag="VD788_17905"
     CDS             3239..4222
                     /locus_tag="VD788_17905"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXV78190.1"
                     /translation="MLIADRPQQGNSVEEEQFEEPILDLERRIEALSGVGEDAATERQ
                     RQKLLAQLEALRERVYSSLTPWQKTLVARHPRRPYTRDYIDHLIEGFHEIHGDRRYSD
                     DPAILCGFGRFRGRAVIVIGHEKGRDTKEKIHRNFGMPRPEGYRKALRAMRLAAKFGR
                     PILTFIDTPGAYPGIGAEERGQAEAIAYNLREMAKLRVPILVTVTGEGGSGGALAIGV
                     GNRVNILQFAIYSVISPEGCAAILWRDASKNREAAQAMRVTADDLARLDVVDEIIAEV
                     TGGAHVDPATQAQKIGDVLEAQLGELDRLSPDELARARYDRFRRLGLFEVV"
     gene            complement(4225..>4884)
                     /gene="mazG"
                     /locus_tag="VD788_17910"
     CDS             complement(4225..>4884)
                     /gene="mazG"
                     /locus_tag="VD788_17910"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HXV78191.1"
                     /translation="DDRPGLCEELGDLLFQIVFLARLAQEEGAFTAEDVVAGIARKMI
                     RRHPHVFGDASVSDANEVLKNWEAIKAEEKREGATSRTGGVGAPSVIDGIPRSLPALL
                     KAQRLGTKAARVGFDWEDAGRVIDKIDEELDELREAVPRGDREAVGEELGDLLFSVAM
                     LARKLDVDPEGALERSNLKFSRRLRWIESRLAEEGRSIADASADEIERLWERAKRADD
                     R"
BASE COUNT          719 a         1675 c         1704 g          786 t
ORIGIN      
        1 cgcgagagcc caacatctgg aagttctgta cgcgaacctc ggtagtgtag cgcttgttgc
       61 cgtcacgatc ggtccactcg cgggtctgca acgagccctc gacgaagatc tgcctaccct
      121 ttcgcaggta ctcgccggcg atctcggctt gcttgccgaa cacgacgagg cgatgccact
      181 cggtccgctc cttgcgttca ccggatgcct ggtcgtacca acgttcggag gtggcgatgg
      241 ggaagttggc gaccggtgtt ccaccctggg tgtacctcac ctccgggtcg cgaccgaggt
      301 ttccgaccag gatcactttg ttgacgccca tctgacactc ctattccatg gtcgaggagg
      361 cttccgcgtc attataccca gggtcaggcg gcgcggcgcg gatcggattc cttgcccgtc
      421 ggcccgtggg acatggtaac gtgggctcaa cccgaaagac gagcgagtcg ccgagatcgg
      481 gtttccgaat tgctacggcc ggagtcaggc gacgattgaa ggtcttgttg cagcgcgtga
      541 gtcgcgcggc ggtacaggtg gacggtcgga ccgtcggacg catcggtcgc gggctgttgg
      601 tgctgttggg cgtggaacgc ggggacgaag aggtcgacgt cgtctacatg gccgacaaga
      661 ccgccgagtt gaggatcttc ccggacgagg atggcaagat gaaccgcagc gtcgaggaga
      721 ccgccggggc ggtgctcgtc gtctcgcagt tcacgttggc cgcgtcgaca cgaaagggac
      781 ggcgcccgtc ctacgtcggc gccgctgatc cgacggacgc cgaggcgcac tacgaacggt
      841 tcatcgctcg cctgcgcgat cgcggtctcg tggtcgagag cggccgattc ggcgcccaga
      901 tgcaggtcga gctggtaaac gacggtccgg tgacgatcct gctcgatccc cgctcgacgg
      961 gcgcagaagc atgacgcgca agcgcgtcac ccgcgccgag ccccgtgcgc caggtgtctg
     1021 ggtcgaacgt tatctcgatt acctgacggt cgaacgcggc ctctcgccca acacactcgc
     1081 cgcgtaccgc gcggacctga cgcgcctgcg cgggttcctg ccgcgcgaga tggcgctcga
     1141 gcgagccggg cgcgacgacc ttcggcgctt tctccaccag atgcgtgccg acggccgctc
     1201 gccgcgctcg gtggcgcgtt gggtcgtcgc ggtcaagggg ttcttcggtt atctggtcgc
     1261 cgagggagcg ctggcggtcg atcccgccgc gcaactcgat ccgccgcgca tctggcgctc
     1321 tctgcccaag gtgctgacgt tcgaggaggt cgaaagcttg ctcgccggtc ccgaccgctc
     1381 ggagccccgc ggcttgcgcg atgccgcgat gatggaggtg ctgtacgcga ccggcttgcg
     1441 ggtcagcgag ctgctgcgtc tgcatctcgg cgacctgcac ctcgacgcgg gctaccttcg
     1501 ctgttggggc aagggtgcga aggagcgcgt cgttcccctc gggggcgagg ccgacgggac
     1561 gttgcaacgt tacttggccg aggcccgccc ggtgatgctc ggccaacggc gcagtgactc
     1621 gttgttcgtc aacgcgcgcg ggacgccgct gacccggcag ggattctgga agatgatcaa
     1681 gcgatacggc atccaggccg ggatcacgac gccgttgtcg ccgcacgtcg tacgccactc
     1741 gttcgcgacg catctgctcg agaacggcgc ggacctgcgc gcggtccagg tgatgctcgg
     1801 gcacgcggat atctcgacga cgcagatcta cacccacgtc aaccgggagc gcctcaggcg
     1861 actgtacgag gacttccatc ctcgcgcgtg accgagggtg cgtcgatgac gatcagaggc
     1921 gcgacgccgg cacggatcga ccgccgccgg ctgatccgcg gtcttggccg ccggcgggct
     1981 gcgctcctct tcggcgtcgg ctcgctcgct gccgatctcg ctgccgacgc ctatctcgtc
     2041 ggcggcggcg tgcgggacct gctcctcgac cggaggacga ccgatctgga cctggcgatc
     2101 gagggcgacg cgcgggaggt ggggcgccgg ctggcgcgcg tggagtcggg ggagtaccgt
     2161 gaacacgcgt cgttcggcac ggcaagcgtc acgctcgccg acggcgtgcg tgtcgacctc
     2221 gccggcacgc gatgcgagcg ctatccgcga cccggcgagc tgccgcgggt cgccccgtcc
     2281 gggttgctcg acgacctcgc gcgccgcgat ttcaccgtca acagcatggc gctctccctc
     2341 cggccggagc gattcggccg actcgtcgac ccctacgacg gcgcggacga tctgcggcgt
     2401 ggtcgactgc gcgtgctgca ctcgcgctcg ttcgaggatg acccgacgcg ggtcgcgcgg
     2461 gcgatgcgtc tcgcggcacg gctcggcttc cgcgtcgagc cggcgacgcg gcgctggatg
     2521 gatgcctgcc tcggttccgg ggcgctggcg cggctgtccg cggcgcgccg gcgacgcgag
     2581 gtcgagctgc tgttcgccga atccggctgg cgggcgatcg cacggacgtt gcaccgtcac
     2641 cgcgcatggt cggtgatcga ccgcgccgtg cagccaccgc cggcggcggc ggcgcagctt
     2701 gcacggatcg agcactggta cgactggctg accgcgctcg ccggattcga cccgccgccg
     2761 cgctggatcg cggcgctcgt ccggctcacg cgcgacggca cgtcttccgt tcgccgcgac
     2821 gtcgttcagc gactcgcacc ggaccgccac gcgcgcgcca tgctgctgtc ggccagcgtc
     2881 gaagcgcgag cgctgctcgc gcggctgcgc tcggtgcgag aaccgcaccc gagcacggtg
     2941 ctcggtgccg cggccggtat cagcgaagcc gcctggctcg aggcgctcga ccgtacccgg
     3001 gccggcagga cgcggggcgc gctcgtcgac tttctccgcc acggccgtgg cgtgcgcctc
     3061 gacatcagcg ggcacgacct cgagcgcgcc ggcatcccgc gcgggccgcg catcgccgtc
     3121 gggctggccg cggccctcgc cgccaagatc gacggcaatg cagccgatcg gcaccgggag
     3181 ctcgaggtcg cgctcgtcgc cgcccgccgc gcttgacagc ggagcggtgg ttcgacgtat
     3241 gctgatcgcc gatcggccgc aacaggggaa cagcgtggaa gaggaacagt tcgaggagcc
     3301 gattctcgac ctcgagcgcc ggatcgaagc gttgtcgggc gtgggcgagg acgcggcgac
     3361 cgagcggcaa cggcagaagc tgctggcgca gctcgaggcg ctgcgcgaac gcgtctactc
     3421 gtccttgacc ccgtggcaaa agacgctcgt cgcgcgacat ccccgacgtc cctacacccg
     3481 tgactatatc gaccacttga tcgaaggctt ccacgagatc cacggcgacc ggcgctactc
     3541 cgacgacccg gcgatcctct gcgggttcgg tcggttccgc gggcgtgcgg tgatcgtcat
     3601 cgggcatgag aaggggcgtg acaccaagga gaagatccac cgcaacttcg gcatgccgcg
     3661 gcccgagggg taccgcaagg ccctgcgtgc gatgcgattg gccgcgaagt tcggccggcc
     3721 gatcctgacc ttcatcgaca cgccaggagc ctaccccggt atcggagccg aggagcgagg
     3781 tcaggccgag gcgatcgcct acaacctgcg cgagatggcc aagctcagag tcccgatcct
     3841 ggtgaccgtc accggtgagg gcgggagcgg gggagcgctc gcgatcggcg tcggcaatcg
     3901 cgtcaacatc ctgcaattcg ccatctactc ggtgatcagc cccgaggggt gcgccgcgat
     3961 cctatggcgc gacgcgagca agaatcgtga agcggcgcag gcgatgcgcg tcacggcgga
     4021 cgatctggcg cggctcgatg tcgtcgatga gatcatcgcc gaggtgaccg gcggggccca
     4081 cgtcgacccc gcgacccagg cgcagaagat cggcgacgta ctcgaggctc agctcggaga
     4141 gctcgatcga ctctcgcccg atgagctggc gcgggcccgc tacgaccgtt ttcggcgact
     4201 cggtctgttc gaggtcgtct gagttcagcg gtcgtcggcc cgcttggccc gctcccacaa
     4261 tcgctcgatc tcgtcggccg aggcgtcggc gatcgagcgg ccctcctcgg ccagccggga
     4321 ctcgatccag cgtaggcggc gggagaactt gaggttcgac cgttcgagcg ccccctcggg
     4381 atcgacgtcg agcttgcgcg ccagcatcgc gacgctgaac aacaggtcac ccagctcctc
     4441 gccgacggcc tcgcggtctc cacgcgggac cgcctcgcgc agttcgtcga gttcttcgtc
     4501 gatcttgtcg atgactcgcc ccgcgtcctc ccagtcgaac ccgacccgcg ccgccttggt
     4561 accgagccgc tgggccttga gcagggcggg caggctgcgc gggatgccgt cgatcaccga
     4621 cggcgcgccg acgccgcccg ttcgcgacgt cgcgccctcg cgtttctcct cggccttgat
     4681 cgcctcccag tttttcaaca cctcgttcgc gtcggacacc gaggcatcgc cgaagacgtg
     4741 cgggtggcgc cggatcatct tccgcgcgat tccggcgacc acgtcctcgg cggtgaacgc
     4801 gccctcctcc tgcgccaggc gggcgagaaa aacgatctgg aacagcagat cgccgagttc
     4861 ctcgcacagg ccgggacgat catc
//
LOCUS       DASPGN010000617         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1638343, whole genome shotgun sequence.
ACCESSION   DASPGN010000617 DASPGN010000000
VERSION     DASPGN010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638343"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..543)
                     /locus_tag="VD788_17915"
     CDS             complement(250..543)
                     /locus_tag="VD788_17915"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HXV78192.1"
                     /translation="MGVFIALLDYTDQGIREIKDSPHRADRFNAMAERAGVKILAQYW
                     TIGSHDGVLVMEAPNDEVAASILLALGAEGNVRTTTLRAFEWAEAQGLIERSD"
     gene            complement(989..1600)
                     /locus_tag="VD788_17920"
     CDS             complement(989..1600)
                     /locus_tag="VD788_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78193.1"
                     /translation="MARHSGLAACVVGILLMNLSFPGVAAGMKVDINFVLGGKALDED
                     DWDDDADSQGMIGIETTFGNPGWPVAVALDYLLSGQSDERVVTSPVLGEVDLLQATAE
                     LDAGVRKIWTVGRARPFVGGGLGILTARQETNVGLRTIDEQDGTVGIWLNGGCFWRLT
                     RRFNLGVDLRLSAGEVELFGRDVSAGGNSLGLLLGWGWGGQSR"
     gene            complement(1603..>2293)
                     /locus_tag="VD788_17925"
     CDS             complement(1603..>2293)
                     /locus_tag="VD788_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78194.1"
                     /translation="WRRWCEIDAPYEAASIKVKLADALLAGGDERAARLHLSAALAVF
                     DRLGARLDAGRARERLARQPDPAPRSAERARAWAFVEIVGFDRLAAALGAEGWSDFEG
                     WLKRILGNCCIDHGGSPVPSDPAGSFGAEFSRAEDAVRCGVHIQRSLREHRARHGFAP
                     PIRAGIVEGAADDGIDRARELAARAATGEILTSRAIAERCDYPTRALVDPPETVRVLW
                     QDASGARSEKG"
BASE COUNT          364 a          822 c          767 g          340 t
ORIGIN      
        1 gctgcaagtc ggcctgctcg acgtggtagg cgagcgggtc gatcccgccg accgtcttct
       61 cgggaaaggt ttcccgcaca tcgcccaccg cgaacgccgt ggtcaccccg acgaacgcga
      121 gcaggacgct cgacagcacg ctgctggtgc gtgtaggtct catgctcaac ccccctgatc
      181 gttgcggttt gtgaacgacg cggaccgtac gccgaatccg ggcggggggt caagcggaat
      241 gtctcgacct caatccgacc gttcgatcag cccctgcgcc tcggcccact cgaaggcccg
      301 caaggtcgtc gtccgcacgt tgccctccgc accgagggcc agcaggatcg aggcggccac
      361 ctcgtcgttg ggcgcctcca tcaccagcac tccgtcgtgg gagccgatcg tccagtactg
      421 tgcgaggatc ttgaccccgg cgcgctcggc catcgcattg aaccgatcgg cgcggtgcgg
      481 cgagtccttg atctcgcgga tcccctgatc ggtgtagtcc aatagcgcga tgaagacacc
      541 catcgtggcc tccctccgtt cgtcgcgtcc cgaccccgcg tcaatctaac acgacgccgc
      601 ggcgggcgcg ggtccctgcc cgacggccgc acgaccggga ggaacggcgg ctagcgaaac
      661 ttcgatcgga tctcggtgat gtagaccgcg acacgggggc gccgggcgcc gcactggggg
      721 cagcgctccg ctttcgctcg aaggaaggcg ccgcagcggg tgcacggtcg gttggcgagg
      781 aggcggtgaa gcggggagtc cgcaacaagc ggcggtcttc tcttccagaa tcgtgtgacg
      841 atcgacatcg gggtacttcc gttgcttgcg aatacactgg ctcggcgcgg cgcgccatag
      901 ccgatcccgt aagccatccg acgaactttc cgtcggcgcg gcgttgtctc gattgtcggc
      961 cgcacgccgg ggatcgctcg gggcgaggtc agcgcgactg gccgccccag ccccaaccga
     1021 gcaacagacc gaggctgttg ccaccggcgc tcacgtcccg accgaacagc tcgacctcgc
     1081 cggccgacag ccgaagatcg acgcccaggt tgaagcgcct cgtcagtcgc cagaagcaac
     1141 cgccgttcaa ccagataccg accgtgccgt cctgctcgtc gatcgtccgc aacccgacgt
     1201 tcgtctcctg ccgggcggtg aggatgccga gcccgccgcc gacgaacggt cgcgcgcggc
     1261 cgaccgtcca gatcttgcgc acgccggcgt cgagctcggc ggtggcctgc agcaggtcga
     1321 cctcgcccaa caccggcgag gtaacgactc gttcgtcgct ctgccccgag agcaggtagt
     1381 cgagcgccac cgcgaccggc caaccggggt tgccgaaggt cgtttcgatg ccgatcatcc
     1441 cctgcgaatc ggcgtcgtcg tcccaatcgt cctcgtcgag cgccttgcca ccgagcacga
     1501 agttgatgtc gaccttcatg cccgcggcga cgcccggaaa tgagaggttc atcagcagga
     1561 ttccgacgac acacgccgcg agcccggaat gccgcgccat cctcagccct tctcggatcg
     1621 agccccgctc gcatcttgcc acagaacgcg gacggtctcg ggcggatcga ccagtgcgcg
     1681 cgtcggatag tcgcagcgtt cggcgatcgc ccgcgaagtc aggatctctc ccgtggcggc
     1741 tcgcgccgcc agctcgcgcg cgcggtcgat tccgtcgtcg gcggcgccct cgacgatccc
     1801 ggcgcggatc ggcggcgcga aaccgtgccg tgcccggtgc tcgcgcagcg aacgctggat
     1861 gtggacgccg cagcggacag cgtcctccgc gcgggagaac tcggcgccga agctgccggc
     1921 cgggtccgat ggtaccggag agccgccgtg atcgatgcag cagttgccga ggatccgttt
     1981 gagccagccc tcgaagtcgg accagccttc ggcgccgagc gcggccgcga gacgatcgaa
     2041 accgacgatc tcgacgaagg cccaggcgcg cgcccgctcg gcggaccggg gcgccgggtc
     2101 gggttgccgg gcgagtcgct cgcgagcgcg cccggcatcg agacgcgcgc cgagacgatc
     2161 gaacacggcg agcgccgcgg acaggtgtag acgcgcggcc cgctcgtcgc cgccggcgag
     2221 caatgcgtcg gccagcttga ccttgatcga cgccgcctcg tacggcgcgt cgatctcgca
     2281 ccagcgccgc cag
//
LOCUS       DASPGN010000618         6022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_700953, whole genome shotgun sequence.
ACCESSION   DASPGN010000618 DASPGN010000000
VERSION     DASPGN010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700953"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VD788_17930"
     CDS             <1..1197
                     /locus_tag="VD788_17930"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV78195.1"
                     /translation="GTDAGWPQWGGPRGDFTVGTVGLADSWPPAGPPRLWRRDLGRGY
                     SAIAARHGRLYTMYRDGLHDVVVALDAGTGETSWEHRYPGRVHPGNVTQFGVGPNAMP
                     LALDDRVVTLGYGGMLNCLDAATGEPLWRHDLVADYDGAVPEFGYSASPVLLDGKVIV
                     LVGGERQGVIALDPADGAVAWAGPPSSVSYATPIGIELDGQRQIVYFTADEVVGLDAN
                     DGAKLWSHPCVNQYRNNATPPQWDPRSALLWVATQLDGGGRALRLRRTEAGTAVEEAW
                     FNDEIKIHFWNSVRVGDHVYASIGGQVTLLAAVELATGAIKWRERGYSKINAIYVGDR
                     LIFLDEKGLLGMARVSPEGFDQLAETRISAGKTWTVPTLAGTTLFVRDDHSIQAFDLG
                     PGRPAG"
     gene            1217..1555
                     /locus_tag="VD788_17935"
     CDS             1217..1555
                     /locus_tag="VD788_17935"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXV78196.1"
                     /translation="MATKKQKIGKAVVLDVSGELKLGPSIAALRRVFRGCIDDGERYF
                     VFNMLKVPWIDSSGIGEIVACYKRTRDVHGELKVVMKGKAHDLFTFYELHKVCELHET
                     LNDALGSFAK"
     gene            complement(1524..2456)
                     /gene="rarD"
                     /locus_tag="VD788_17940"
     CDS             complement(1524..2456)
                     /gene="rarD"
                     /locus_tag="VD788_17940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HXV78197.1"
                     /translation="MSDARDRRSPRSGVLYALAAFGIWGVNPLYFDAVRQLPPLELLA
                     HRIVWSVPLTALLITAVADWSALRAAVADRRTLATLAISSLLVSLNWLTFIYAIATQR
                     VVEASLGYFINPLVNVLLGLLFLRESLSRPQAVAVFLALGGTLELTWSHGRLPWIALV
                     LALSFGLYGLLRKTVAIEAVNGLFVETLLLSPLALGYLAALAGGSRGTFGTRGIRLDL
                     LIVCAGVVTAVPLVLYTSASRRLRYITIGVLQYLAPSIQLTLGVFWFGEPFTRAHSVA
                     FGLIWSGLVLFVVDAARTARATTRPTSRTTPTRR"
     gene            complement(2463..2984)
                     /locus_tag="VD788_17945"
     CDS             complement(2463..2984)
                     /locus_tag="VD788_17945"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HXV78198.1"
                     /translation="MKIRNVVALGLVLLSFGLLVPGLILPLITIRASFNFMGNTIDVF
                     NQTRSIVQSIKDLHESGNDFVAGLILLFSVLVPLFKGALLGAVALARSPSWRRRLFVF
                     VRGISKWAMADVFAVGVYVAFLAAKASENLDARVGTGFYWFVAYCLVSLAALQFMWVA
                     PANPPSSAGGRAD"
     gene            3358..>6022
                     /locus_tag="VD788_17950"
     CDS             3358..>6022
                     /locus_tag="VD788_17950"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXV78199.1"
                     /translation="MRTSVVVIAVLCFVLCAPPASARTGALTVTVTDPDGRPMPQVTV
                     TLSNADGLIKSTVARTDEAGRVAFPVLAAGDGYRIEVSAPGFAAVAVAQLRVPIGETT
                     SVPVRLAPDIAERVSVTARTDVVDLTETQASTRFTDEFVRDLPVAGRFYQNMLTLAPG
                     VEDSDGDGNPTVHGSRARDFKAVVSGVSNVDPLTGQWMSRINPNSIEEMEIITAGAGV
                     EFGRAQGGYARILQKQGSNSLEGVFDFLYRSSALDQPSDVSNVGDPQYESYQTGFQLS
                     GPIVRDRLWYRLSHEWIRREDPVDIAIGTEVVETDQATHSDQLTWQVSPRNKLSFSYQ
                     GDPLEISNFGVSNIVSPESSQRREFGGETYSVRWTAPYSAKVLIESQVAWQELDIGIL
                     PTTAGVLNDCVTGLPALQRAECLDTTRSRTSGSASRTLDDRRQRLTVRGDVTTFVGRF
                     LGATHQLKAGVIVENERYFRSLEQRPRTRLFVVPPFTIENGELQQEGTFLAAQIAVPE
                     TDDVRATGVSFGLYIEDQLKPAQNLSITLGLRFDREEIDSSGHSPFDPLAEQATYFEQ
                     VESGAALPSEALRQTFTAFEATGDLTAQLARLLGLSDNDITQVLSPIAVQSEFWAHTR
                     RLEDLHLVNDNLAPYLGLSWDPWKDGKTKLALVASRHYNNIPLSVPLVELEPVEADLT
                     FRAFPGTRLITRPFGGVNLGLSTQLVDRDLATPYQDELYLSAERQVASETSLRLSYIK
                     RLYRDQLQDRDINRFTGDFGICRHATPTNRSPIRATLETEFPDTFPGDGVIDDCTGRT
                     EPIGDASGGGTNFLQRPDGFDDLYIANPGWVDVLLVGNFNRADYDAVVFEVIRRLYRD
                     WEMQLSYTYAEAVGDGEDFLQQFGNDNALAED"
BASE COUNT         1055 a         2016 c         2075 g          876 t
ORIGIN      
        1 gggaccgacg cgggctggcc ccagtggggc ggtccgcgcg gtgatttcac cgtcggcacc
       61 gtcgggctgg ccgacagctg gccgccggcc gggccgccgc gcctgtggcg cagggatctg
      121 ggtcggggtt actccgcgat cgccgcgcgg cacgggcgcc tgtacacgat gtatcgcgac
      181 ggcttgcacg acgtcgtcgt ggcgctcgac gccggcaccg gcgaaacctc gtgggagcat
      241 cggtacccgg ggcgggtcca tcccggaaac gtcacgcagt tcggcgtcgg acccaacgcg
      301 atgccgctgg cgctcgacga tcgggtcgtc acgctcggtt atggcggcat gctcaactgt
      361 ctcgacgcgg ccaccggcga gccgctgtgg cggcacgacc tggtcgccga ctacgacgga
      421 gcggtgccgg agttcggcta ctcggcgagc ccggtgctgc tcgacggcaa ggtgatcgtg
      481 ctggtcggcg gcgaacgcca gggcgtcatc gcgctcgacc ccgccgatgg cgccgtcgcc
      541 tgggccggcc cgccgagcag cgtgagctac gccacaccga tcgggatcga gctcgacggc
      601 cagcggcaga tcgtttactt caccgccgac gaggtggtcg gtctcgacgc gaacgacggc
      661 gcgaagttgt ggagccaccc gtgcgtcaat caataccgca acaacgccac gccgccgcag
      721 tgggacccgc ggtcggcgct gttgtgggtc gcgacccagc tcgacggcgg cggccgcgcc
      781 ctcaggctcc ggcggacgga ggccggcacc gccgtcgagg aggcgtggtt caatgacgag
      841 atcaagatcc acttttggaa ctcggtgcgg gtcggcgacc acgtctatgc ctcgatcggc
      901 ggacaggtca cgctgctcgc cgcggtcgag ctggcgacgg gggcgatcaa gtggcgcgag
      961 cggggctact cgaagatcaa cgcgatctac gtcggcgacc gcttgatctt tctcgacgag
     1021 aagggcctgc tcggcatggc gcgcgtctcg cccgaggggt tcgaccagct ggccgagacg
     1081 cggatctccg ccggcaagac gtggaccgtt ccgacgctcg ccgggacgac gctgttcgtc
     1141 cgcgacgatc attcgattca ggcgttcgat ctcggtcccg gcagaccggc aggttgagcg
     1201 cggcggaggg cgagaggtgg cgaccaagaa acagaagatc ggcaaggcgg tcgtgctgga
     1261 cgtctccggc gagctgaagc tcggcccatc gatcgctgcg ctgcgccgcg tctttcgcgg
     1321 gtgcatcgac gacggtgaac gttacttcgt cttcaacatg ctgaaggtgc cgtggatcga
     1381 cagctccggc atcggcgaga ttgtcgcctg ctacaaacgg acgcgtgacg tccacggcga
     1441 gctgaaggtc gtgatgaagg gcaaggcgca cgacctgttc acgttctacg agctgcacaa
     1501 ggtctgcgag ctgcacgaga cgctcaacga cgcgttgggg tcgttcgcga agtaggccgg
     1561 gtcgtcgccc gcgcggtccg tgcggcatcg acgacgaaca ggaccaggcc ggaccagatc
     1621 agcccgaagg cgaccgagtg ggcgcgggtg aacggttcgc cgaaccagaa cacgccgagc
     1681 gtcagctgga tcgacggcgc gaggtactgc agcacgccga tcgtgatgta acgcaaacgt
     1741 cgcgaggcgc tggtgtacag cacgagcggt accgccgtca ccacccccgc gcaaacgatg
     1801 agcagatcga gacgaatgcc gcgcgtgccg aacgtgccgc gcgaaccgcc ggcgagcgct
     1861 gccaggtagc ccagcgccag cggcgacaac agcagggtct cgacgaacaa gccgttgacc
     1921 gcctcgatgg cgacggtctt gcgcagcaat ccgtacagcc cgaagctcag cgccagcacc
     1981 agtgcgatcc acggcagccg gccgtgactc caggtcagct cgagcgtgcc gccgagggcc
     2041 aagaatacgg cgaccgcctg tggccggctg aggctctcgc gcaggaacag cagccccagc
     2101 agcacgttga ccagcgggtt gatgaagtaa cccaggctgg cctcgacgac gcgctgggtc
     2161 gcgatcgcat agatgaaggt caaccagttg agcgacacga gcagcgagct gatcgcgagc
     2221 gtcgccagcg tccggcggtc ggcgaccgct gcgcgcagcg ccgaccagtc ggcgacggcg
     2281 gtgatcaaca gcgcggtcag cggcaccgac cagacgatcc gatgcgccag cagctcgagc
     2341 ggcggcagct ggcgcaccgc gtcgaagtag agcgggttga ccccccagat cccgaacgcg
     2401 gccagtgcgt agagcacgcc gctgcgcggg gaccgacgat cccgagcgtc gctcaacgac
     2461 gatcagtcgg cgcggccgcc ggcggacgac ggcgggttcg ccggtgcgac ccacatgaac
     2521 tgcagcgcgg cgagcgagac caggcaatag gcgacgaacc agtagaaccc ggtcccgacc
     2581 cgggcgtcga ggttctcgga cgccttggcc gcgagaaacg cgacgtagac ccccaccgcg
     2641 aagacgtcgg ccatcgccca tttgctgatc ccgcggacga agacgaacag tcgccggcgc
     2701 cacgacgggc tgcgcgcgag cgccaccgca ccgagcagcg cacccttgaa caacggcacc
     2761 agcacgctga acagcaggat cagcccggcg acgaagtcgt tgcccgactc gtgcaggtcc
     2821 ttgatcgact ggacgatgct gcgcgtctgg ttgaagacgt cgatcgtgtt gcccatgaag
     2881 ttgaacgagg ctcgaatcgt gatcagcggg aggatcaggc ccgggaccag caggccgaac
     2941 gagagcagga cgaggcccag cgcgacgacg tttcggatct tcatgaaaac cccatgcgag
     3001 acgacgaacc ggtcggaccg cctcgagcgc tgagatctta acccggggtc gcgcaggtgt
     3061 gcggggtctg tcgattcggg aagaggggcc gagcccggtt gctccgtcgc tgctgtgatt
     3121 ggtccgtcgg gcgcggcccc tgcggtgacc cctgcggagg tcgccacgga tgcactagac
     3181 gcccggcggc gccccgcgtc aactgaagat ccgggaaaaa tcgggtcttt tgagtcggtt
     3241 gcgcgggaca gccggcgcga atcggggtat acaagtccgc cgggcggttc ctccgcggcg
     3301 cacgcggtcg aagccggttt cgtggtgtat catccgcggc ttcccgagaa aacagagatg
     3361 cgaacgtcgg ttgtcgtcat cgcggtcctg tgcttcgtgc tgtgcgcgcc gccggcgtcg
     3421 gcccggaccg gggccctgac cgtgacggtg accgatcccg acggccgccc gatgccgcag
     3481 gtcacggtca cgctgagcaa cgcggacggg ttgatcaagt cgaccgtggc gcggaccgat
     3541 gaggccggaa gggtggcctt tccggtgctg gccgcggggg acggctaccg catcgaggtc
     3601 tcggcgcccg ggttcgccgc ggtcgcagtc gcccagttgc gcgtgccgat cggcgagacg
     3661 acctcggtac cggttcggct cgcgcccgac atcgccgagc gggtcagcgt gacggcccgg
     3721 acggacgtcg tcgatctgac cgagacccag gcatcgacac gcttcaccga cgagttcgtc
     3781 cgcgacctac cggtggccgg ccgcttctac cagaacatgc tgaccttggc gccgggcgtc
     3841 gaggattccg acggcgacgg caacccgacg gtccacggct cgcgcgcgcg ggatttcaag
     3901 gccgtcgtca gcggcgtcag caacgtcgac ccgctgaccg gccagtggat gagccggatc
     3961 aatcccaact cgatcgagga gatggagatc atcaccgccg gtgcgggggt ggagttcggc
     4021 cgcgcgcagg gcggctatgc ccgcatcctg cagaaacagg gaagcaacag cctcgagggg
     4081 gtgttcgact tcctctatcg ctcgagcgcg ctcgaccagc cgtccgacgt gtcgaacgtc
     4141 ggcgacccgc agtacgagtc gtaccagacg ggattccagc tatcgggccc gatcgtcagg
     4201 gaccgactgt ggtaccggct gtcgcacgag tggatccgcc gcgaggatcc ggtcgacatc
     4261 gcgatcggca cggaggtcgt cgagaccgac caggcgacgc actcggacca attgacctgg
     4321 caggtcagcc cgcgcaacaa gctgtccttt tcctatcaag gcgatccgct cgagatcagc
     4381 aatttcggag tcagcaacat cgtctcgccg gagtcgagcc agcgccgcga gttcggcggc
     4441 gaaacctaca gcgtgcggtg gaccgcgccg tactcggcca aggtgctgat cgagagccag
     4501 gtggcctggc aagagctcga catcggcatc ctccccacca ccgcgggcgt gctgaacgac
     4561 tgcgtcaccg ggctgccggc gctgcagcgc gccgaatgcc tcgatacgac gcggtcgcgc
     4621 accagcggct cggcgtcgcg cacgctcgac gaccgccgcc agcgcctgac cgtgcgcggc
     4681 gacgtgacga cgttcgtcgg ccgttttctc ggggcgacac accagctcaa ggcgggagtg
     4741 atcgtcgaga acgagcgcta cttccgctcg ctcgagcagc gcccgcgcac gcggctgttc
     4801 gtcgtcccgc cgttcacgat cgagaacggc gaattgcagc aggagggtac gttcctcgcg
     4861 gcgcagatcg ccgtccccga aacggacgac gtgcgggcga cgggcgtcag cttcggtctg
     4921 tacatcgagg accagctcaa gccggcgcag aatctctcga tcacgctcgg cttgcgcttc
     4981 gaccgcgagg agatcgactc gtcgggtcac tcgccgttcg acccactggc ggagcaggcc
     5041 acctacttcg agcaggtgga gagcggggcc gcactgccgt cggaggcact gcggcagacc
     5101 ttcaccgcgt tcgaggcgac gggtgacctg accgcgcagc tggcgcgctt gctcggactg
     5161 agcgacaacg acatcaccca ggtgctctcg ccgatcgcgg tgcagagcga gttttgggcg
     5221 cacacgcgcc ggctcgagga cctgcatctg gtcaacgaca acctcgcgcc ctatctcgga
     5281 ctgagctggg acccgtggaa ggacggcaag accaagctcg cactcgtcgc cagccggcac
     5341 tacaacaaca tcccgctcag cgtcccgctg gtcgagctcg agccggtcga ggccgacctg
     5401 accttccgcg ccttccccgg cacgcgactg atcacacgcc cgttcggcgg ggtcaacctg
     5461 ggcctcagca cccaactcgt cgaccgcgac ctggcgacgc cctaccagga cgagctgtat
     5521 ctcagcgccg agcgtcaggt ggcgtccgag acctcactgc gcctgtcgta catcaagcgg
     5581 ttgtaccgcg atcaactgca ggaccgggac atcaaccgct tcaccggaga cttcggcatc
     5641 tgccgccatg ccacgccgac caaccggtcg ccgattcgcg cgacgctcga gacggagttc
     5701 cccgacacct tccccggcga cggtgtgatc gacgattgca ccggacggac cgagccgatc
     5761 ggggacgcga gcggaggcgg gaccaacttt ctgcagcgcc ccgacggctt cgacgacctg
     5821 tacatcgcca acccgggctg ggtcgacgtg ctgctggtcg gcaacttcaa ccgcgccgat
     5881 tacgacgctg tcgtgttcga ggtgattcgg cggctgtacc gcgactggga gatgcagctg
     5941 tcctacacct acgccgaagc ggtcggcgac ggcgaggatt tcctccagca gttcggcaac
     6001 gacaacgcgc tggccgagga cg
//
LOCUS       DASPGN010000619         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1520035, whole genome shotgun sequence.
ACCESSION   DASPGN010000619 DASPGN010000000
VERSION     DASPGN010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520035"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..422
                     /gene="rplV"
                     /locus_tag="VD788_17955"
     CDS             69..422
                     /gene="rplV"
                     /locus_tag="VD788_17955"
                     /inference="COORDINATES: protein motif:HMM:NF012462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXV78200.1"
                     /translation="MIATAKLKYLGVSSQKTRLVVDQVRGRNVGEALAILHYSPKLVA
                     RDLEKLVKSAVANAQQSDPNVDVDQLVIARAMVDEAPSMKRARAQSMGRIFRVLKRAC
                     HVTIDLDLSGKSHGH"
     gene            439..1092
                     /gene="rpsC"
                     /locus_tag="VD788_17960"
     CDS             439..1092
                     /gene="rpsC"
                     /locus_tag="VD788_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HXV78201.1"
                     /translation="MGQKVHPYGFRIGFNKTWRSRWYAEKKYPELLHEDLKLRDELKQ
                     RLGHAGVSAIEIERAANKLKVNILTSRPGIIIGRKGVEVDRLKDEIRRRTKRDVFINI
                     LEIDKPEIDAQLVAEAIGMQLVKRVAFRRAMRKAVESAQRFGAKGIKVRVAGRLGGAE
                     IARAEWYLEGQLPLHTLRADVDYGFAEARTTYGVIGIKVWIYRGEERDIRVPEGASR"
     gene            1114..1524
                     /gene="rplP"
                     /locus_tag="VD788_17965"
     CDS             1114..1524
                     /gene="rplP"
                     /locus_tag="VD788_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXV78202.1"
                     /translation="MLMPSKVKFRKQQRGRRAGKANAGAKVDFGDFGLKALECGWLTA
                     RQIEAGRVAMTRFIKRGGKIWIRVFPDKPITKKPTEVRMGKGKGAPEAWVAVIRPGRV
                     LFEMEGVSEEIAREAMRLAAHKLPFKTRFVSRHP"
     gene            1582..1785
                     /gene="rpmC"
                     /locus_tag="VD788_17970"
     CDS             1582..1785
                     /gene="rpmC"
                     /locus_tag="VD788_17970"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXV78203.1"
                     /translation="MSIEDLDKEARQLREAIWKLRLQLTTGQLQDPQKVTMARKELAR
                     VLTVRREQEISAGGRTRRRRSGR"
     gene            1782..2078
                     /gene="rpsQ"
                     /locus_tag="VD788_17975"
     CDS             1782..2078
                     /gene="rpsQ"
                     /locus_tag="VD788_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXV78204.1"
                     /translation="MNEASPAAVRRRKATKIGVVTSNKMDKSVVVRVERTVLHDRYKK
                     HVQRSARFMAHDERNECKIGDTVEIVESRPLSARKRWRVRRIVRPADTDWTAAE"
     gene            2093..2461
                     /gene="rplN"
                     /locus_tag="VD788_17980"
     CDS             2093..2461
                     /gene="rplN"
                     /locus_tag="VD788_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXV78205.1"
                     /translation="MIQMQSVLDVADNSGAKKIACITVLGGSSGRYAGIGDIITASVK
                     EAAPDGNVKKGQVVKAVVVRTVKTHRRRDGSYIRFDENAAVLINDAREPIGTRVFGPV
                     ARELRDKKFMKIVSLAPEVL"
BASE COUNT          496 a          717 c          921 g          368 t
ORIGIN      
        1 gagttcgcgc cgacgcggac gttcaaggga cacgccggca agggcgcgag ctagcgcgga
       61 ggttcgacgt gatcgcaacg gcaaaactga agtacctcgg cgtctcgtcg caaaagaccc
      121 ggctcgtcgt cgaccaggtc cgcggccgca acgtcggcga agcgctggcg atcctgcact
      181 actcgcccaa gctcgtcgca cgcgacttgg agaagttggt caagtcggcc gtggcgaacg
      241 cccagcagtc ggatcccaac gtcgacgtcg accagttggt gatcgccagg gcgatggtcg
      301 acgaggcacc gtcgatgaag cgcgcgcgcg cgcagagcat gggccggatc ttccgcgtgc
      361 tcaagcgggc ctgtcacgtc acgatcgatc tggatttgtc cgggaaatcg cacggtcact
      421 gagcgcggag ggagtccggt gggtcagaaa gtacatcctt acggttttcg catcggcttc
      481 aacaagacgt ggcgctcgcg gtggtacgcc gagaagaagt accccgagct gttgcacgag
      541 gacctcaagc tgcgcgacga gctgaagcag cggctggggc acgccggcgt ttccgcgatc
      601 gagatcgagc gcgccgcgaa caagctgaag gtcaacatcc tgacgtcgcg gccggggatc
      661 atcatcggcc gaaaaggggt cgaggtcgac cgactgaagg acgagattcg ccggcgcacc
      721 aagcgcgacg tgttcatcaa catcctggag atcgacaagc cggagatcga cgcgcagctg
      781 gtggccgagg cgatcggcat gcaactggtc aagcgcgtcg cgttccgccg ggcgatgcgc
      841 aaggcggtcg aatcggctca acgattcggc gccaagggga tcaaggtccg ggtggccggc
      901 cgactgggcg gggcggagat cgctcgtgcc gagtggtacc tcgaaggtca gctcccgctg
      961 cacaccctgc gcgccgacgt cgactacggc ttcgccgagg cgcggacgac gtacggcgtg
     1021 atcggcatca aggtctggat ctatcgcggt gaggaacggg acatccgcgt gcccgagggc
     1081 gcgtcgcgct agccgccggg agagggttgc gtcatgttga tgccgagcaa ggtcaagttc
     1141 aggaaacagc agcgtggccg ccgcgccggc aaggccaacg cgggggcgaa ggtcgacttc
     1201 ggcgacttcg ggctgaaggc gctcgagtgc ggttggctga ccgcgcgcca gatcgaggcc
     1261 ggtcgcgtgg cgatgacacg gttcatcaag cgcggcggca agatctggat ccgggtcttc
     1321 ccggacaagc cgatcacgaa gaagccgacc gaggtgcgca tgggaaaagg caagggcgcg
     1381 ccggaggcgt gggtcgccgt gatcaggccg ggccgggtgc tgttcgagat ggaaggtgtg
     1441 tcggaggaga tcgcgcgcga ggcgatgcgg ctggcggccc acaagctgcc gttcaagacg
     1501 cggttcgtct cgcggcatcc gtaggccggc gggacctggt ggagcgaggc aatggcgctt
     1561 gatatcgcga agatccgaaa catgtcgatc gaggatctgg acaaggaagc tcgacagctg
     1621 cgcgaggcga tctggaagct ccgcctgcag ttgacgacgg ggcagctcca ggacccgcag
     1681 aaggtgacga tggctcgcaa ggagctggcg cgcgtgctga cggtcaggcg cgagcaggag
     1741 atcagcgccg ggggacgaac ccgtcggcga cggagtggca gatgaacgag gcaagcccgg
     1801 cggccgtgcg acggcgtaag gcgaccaaga tcggcgtggt cacctcgaac aagatggaca
     1861 agtcggtggt ggtgcgtgtc gaacgcaccg tgctgcacga tcggtacaag aagcacgtcc
     1921 agcgctctgc ccgattcatg gcgcacgacg agcgcaacga gtgcaagatc ggcgataccg
     1981 tcgagatcgt cgagtcgaga ccgttgtccg cgcggaaacg ttggcgagtg cgtcgcatcg
     2041 tgcggccggc tgacaccgat tggacggcag cggagtaggg aggggtcgcg acgtgatcca
     2101 gatgcaatcg gtcctcgatg ttgccgacaa ctcgggggcc aagaaaatcg cgtgtatcac
     2161 cgtgttgggc ggctcgagcg ggcgctacgc cggcatcggt gacatcatca ccgcgtcggt
     2221 caaggaagcg gcgccggacg gcaacgtgaa gaagggccag gtcgtcaagg cggtcgtcgt
     2281 acggacggtc aagacgcatc gccgtcggga cggatcgtac atccgcttcg acgagaacgc
     2341 ggcggtgttg atcaacgacg cacgcgagcc gatcggcacg cgcgtcttcg gtccggtggc
     2401 gcgcgagctg cgggacaaga agttcatgaa gatcgtctcg ttggctccgg aagtgctgtg
     2461 agcacgcgga ggagcaactg atgggcaaga cccgcgtcaa ga
//
LOCUS       DASPGN010000620         8514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_1638537, whole genome shotgun sequence.
ACCESSION   DASPGN010000620 DASPGN010000000
VERSION     DASPGN010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1638537"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1622
                     /gene="speA"
                     /locus_tag="VD788_17985"
     CDS             <1..1622
                     /gene="speA"
                     /locus_tag="VD788_17985"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HXV78206.1"
                     /translation="IKVNQQRRVVEEMVRFGAEDSIGLEAGSKPELLIALAQLDTPGA
                     LIICNGYKDRAYVETALLAQRLGRTPIIVIDRYHELDLVLKTASELGIRPHIGLRARL
                     TTKGAGKWVESTGDRSKFGLSAEEIVDAVERLRAEDMLDCLELLHFHIGSQITAIRAH
                     KDALREASRIFAGLHEMGARPTIVDVGGGLGVDYDGSQTNFHSSMNYTVEEYANDVVA
                     ALQEAADEKGIPHPDIVTESGRAMVAHHSVLVFDVLGVNEMAMGKAPEPAGEDEPKVV
                     RDLGEVLGSVRRKNVLESYHDALQLKEEAATLFSLGYLDLRGRARVERMFWACCEKIL
                     RIARDMETVPEELGDLERGLADTYYGNFSVFQSAPDHWAVDQLFPVMPIQRLAEEPTE
                     QAVLADLTCDSDGRLDRFIDARGTQRSIRLHPVLTDEPYYIGIFLVGAYQEILGDLHN
                     LFGDTNAVHVNLDDDGNPTISQIVRGDTMQEVLEYVQFNTSDLFERLRLAADAALLNG
                     RMSARDVRKLESRYREAMGSYTYLIREDEAF"
     gene            complement(1627..2490)
                     /gene="speB"
                     /locus_tag="VD788_17990"
     CDS             complement(1627..2490)
                     /gene="speB"
                     /locus_tag="VD788_17990"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HXV78207.1"
                     /translation="MADSIINCADPARADVVLIGAGYDGTSSFGKGADRGPAAVRDCL
                     DHQIEFDDRISGGNPAERLAIAWVDADDLAGLPPAAMVERLERRYRAHDGRLRVLVGG
                     EHSISNAAFRAWAGEAGEITVVQIDAHADLRDDDSDYNDEPHGRFAHCSVMRRAHEAG
                     YRLCQIGIRAYSAAERELFDDPRITVWEWGREPAPAERIVDSIATERVYLTLDVDGLD
                     PALAPATGTPVPGGLGWYQTTALLRALTRRHRVVAADVVEVCPRPGDRLTEYAAAQLI
                     YSLIGLALPGR"
     gene            complement(2492..3532)
                     /locus_tag="VD788_17995"
     CDS             complement(2492..3532)
                     /locus_tag="VD788_17995"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HXV78208.1"
                     /translation="METKTDKLERRKLLSEPVEHIDIKSFDATEIVRAMRKMSFTARD
                     LGRACEIFQQMLEDPDCSIVLSLAGSTSAGGCMQVYVDMVEHGMIDAVVATGASIIDM
                     DFFEALGFRHYRGSTTADDRTLRELYIDRIYDTYIDENELQACDAKIKEIADSLAPGV
                     YSSRGFIRELGRWLAAGNAKKSGSLVETAYRREVPIFCPAFSDSSAGFGLVIHQHERP
                     AAHVAIDSVRDFRELTEMKIAAETTGMLIVGGGVPKNFLQDTVICAELLGREVPMHKY
                     AIQITVADARDGACSSSTLKEACSWGKVDVTYEQMVYAEATSVIPLLGSHAYHTGAWR
                     DRAPRRWGRLYD"
     gene            3728..4546
                     /locus_tag="VD788_18000"
     CDS             3728..4546
                     /locus_tag="VD788_18000"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXV78209.1"
                     /translation="MSDDYIHGFAPAEQRRLTLMQGILNDEELRHLDLDGVRRILDVG
                     SGLGQMTRALARAAGPAATVVGVERDERQLEEAVRQAEGADEASRVEFRQGRAERLPL
                     TPGERGSFDLAHCRFLLEHVTDPLAVVREMVGAVRAGGRIVLIDDDHELLRLWPVADY
                     VEQVWRIYWESYRDRGHDPLIGRRLAGLLREAGARPTRVTSVFYGAVRGMRLFDPVVD
                     NLASVLGGAAERLDEEGVLARPAMEQALAELDEWRHQASATLWYSLPLAEGTVG"
     gene            complement(4563..5150)
                     /locus_tag="VD788_18005"
     CDS             complement(4563..5150)
                     /locus_tag="VD788_18005"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase family protein"
                     /protein_id="HXV78210.1"
                     /translation="MRIRDGRSWLAVAILLVATGWPTARAEGGEPPDATPRPSALLPI
                     PNARIPLAEVLSGGQPTPDQIEAAARAGFRTVINLRSDGETGFEWEAETVERLGLRYV
                     RIPVAGERDLTRERVEQLDSALSSGLERGPVLLHCASGNRIGAMLALRAAWLGGLPPD
                     EALEFGVASGLTGLYQATSRRLGTAVADPAAAPRE"
     gene            complement(5196..5717)
                     /locus_tag="VD788_18010"
     CDS             complement(5196..5717)
                     /locus_tag="VD788_18010"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXV78211.1"
                     /translation="MTKTVLLVDYDPNSIGRIRRTLGPAGVRVELALDGRSGVAEAQR
                     LRPAVTIVQDLLPGIGGLDVCGQIKHTAESADLPVVILTGPRQHVTLMKTDCDAYIEK
                     PYQDGELLEIVQALLKRSGGVAAGPPRRPERVASRPAIAPVAAPPVCFTEADLDARLD
                     EVLVLAPRVAVAG"
     gene            complement(5846..6253)
                     /locus_tag="VD788_18015"
     CDS             complement(5846..6253)
                     /locus_tag="VD788_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78212.1"
                     /translation="MKRAMLSIGICTLAVGCGGSDSPSQTLSGPITLATVQQQVFSPS
                     CAVTDCHIGPDPEQGLDLSAGSAYANLVNVQAAEINNYMRVEPGNAADSYVYMKITND
                     LRILGDPMPLVGGPLSDSKIRLVERWIDEGANP"
     gene            6512..8116
                     /locus_tag="VD788_18020"
     CDS             6512..8116
                     /locus_tag="VD788_18020"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HXV78213.1"
                     /translation="MSPSRTLRLTLALLLVSAPVAADSGLAVPPSEASRFLSQATFGA
                     NWEEIERTSEVGFEAWLAEQFVRPVGRHQPPLEELARLGVEVENQHRRWVWWERAMQG
                     PDPLRQRVAFALSEIFVVSDNTEAIRDTPIGMANYYDMLLDNAFGNFRELLLDVSLHP
                     IMGVYLSHLRNDRSDPSGGRFPDENYAREVMQLFSVGLFELRPDGTQYTDGDGEPVPT
                     YDNSDITEFAKIFTGLAFDSPAPDFGNGTPVWTRPMRMYQEHHEPGPKYLLRGGFVPP
                     GQTGMHDVADAIDNLFRHPNVGPFIGRRLIQRLVTSNPSPDYIRRVSAAFDDNGAGVR
                     GDMRALVTAILTDPEARQIPNRTETTRGRLRESYLRRVHLARAFDAANLVGSYPITDG
                     GAPTTFGQLPISSPTVFNFFLPDHQPTGELADAGLYAPEFQIMTAVTAIASANALQRQ
                     VEGLMNDDDDPMNEVRLDLSNEIAIAANSRALVDRLDLLLMYGDMSVPMKEILVHALD
                     QLPDPIERVEMAIHLISIAPEYAVQK"
     gene            8133..>8514
                     /locus_tag="VD788_18025"
     CDS             8133..>8514
                     /locus_tag="VD788_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HXV78214.1"
                     /translation="MNDEQNGDEPNGVSRRRFLGQASCAAVGTTAIFSTVLDMSMINV
                     LAQTTPGDYRAMVCLFFGGGIDSFNVVVPRGQAEYDEYAQVRRDLALPQDVLLPITPV
                     TPDGREYGLHPNVPELQALFGQGDL"
BASE COUNT         1292 a         3016 c         2967 g         1239 t
ORIGIN      
        1 cgatcaaggt caaccagcag cggcgcgtgg tcgaggagat ggtgcgcttc ggcgccgagg
       61 actcgatcgg cctcgaggcc ggcagcaagc cggagctcct gatcgccctc gcccagctcg
      121 acacgccggg cgcgctgatc atctgcaacg gctacaagga ccgcgcgtac gtcgagacgg
      181 cgctgctggc ccagcggctc ggccgcacgc cgatcatcgt gatcgaccgc taccacgagc
      241 tcgacctggt gctgaagacc gcctccgagc tcggcatccg cccgcacatc gggttgcgcg
      301 cccggctcac caccaagggc gcgggcaagt gggtggagtc gaccggcgat cgctccaagt
      361 tcgggctctc ggccgaggag atcgtggacg ccgtggagcg gctgcgcgcc gaggacatgc
      421 tggactgcct ggagctcctg cacttccaca tcggctcgca gatcaccgcg atccgcgccc
      481 acaaggacgc cctgcgcgag gccagccgga tcttcgccgg cctccacgag atgggcgcca
      541 ggccgacgat cgtggacgtg ggcggcgggc tcggcgtcga ctacgacggc tcccagacca
      601 acttccactc ctcgatgaac tacaccgtcg aggagtacgc caacgacgtg gtcgcggcgc
      661 tgcaggaggc cgcggacgag aagggcatcc cgcaccccga catcgtcacc gagtccggcc
      721 gggcgatggt ggcccatcac tcggtgctgg tgttcgacgt gctgggcgtg aacgagatgg
      781 cgatgggcaa ggctcccgag ccggcgggcg aggacgagcc caaggtggtg cgcgacctgg
      841 gcgaggtgct cggctcggtg cggcgcaaga acgtgctgga gagctaccac gacgcgctcc
      901 agctcaagga agaggcggcc acgctgttct cgctgggcta cctcgacctg cgcggccgcg
      961 cgcgcgtgga gcgcatgttc tgggcctgct gcgagaagat cctccgcatc gcccgcgaca
     1021 tggagacggt gcccgaggag ctgggagacc tggagcgcgg cctggccgac acctactacg
     1081 gcaacttctc ggtgttccag tccgcgcccg accactgggc ggtcgaccag ctcttcccgg
     1141 tgatgccgat ccagcgcctg gccgaggagc cgaccgaaca ggcggtgctg gccgatctga
     1201 cctgcgacag cgacggccgg ctcgaccgct tcatcgacgc gcgcggcacc cagcgctcga
     1261 tccgcctgca cccggtgctg accgacgagc cgtactacat cggcatcttc ctcgtcgggg
     1321 cgtaccagga gatcctcggc gacctgcaca acctgttcgg cgacaccaac gccgtccacg
     1381 tcaacctcga cgacgacggc aacccgacga tcagtcagat cgtccgcggc gacacgatgc
     1441 aggaggtgct ggagtacgtc cagttcaaca cctccgatct gttcgagagg ctgcgcctgg
     1501 ccgccgacgc cgcgctgctc aacggccgca tgtcggcacg cgacgtccgc aagctcgagt
     1561 cgcgctaccg cgaggcgatg ggcagctata cctatctgat ccgcgaggac gaggcgttct
     1621 gaagcctcaa cggcccggca gcgccagacc gatcaacgag tagatcagct gcgccgcggc
     1681 gtactcggtc agccgatcgc cggggcgcgg acagacctcg acgacgtcgg cggcgacgac
     1741 gcggtggcgg cgcgtcaacg cgcgcagcag cgcggtcgtc tggtaccaac ccagaccgcc
     1801 ggggaccggc gtccccgtcg ccggcgccag cgccgggtcg agcccgtcga cgtcgagcgt
     1861 gaggtagacg cgctcggtgg cgatgctgtc gacgatgcgc tcggccggcg cgggctctcg
     1921 gccccactcc cagaccgtga tccgcgggtc gtcgaacagc tcccgctcgg cggcggagta
     1981 ggcgcggatg ccgatctggc acaaccggta gcccgcctcg tgcgcgcgtc gcatgacgct
     2041 gcagtgcgcg aagcgtccgt gcggctcgtc gttgtagtcg gaatcatcgt cgcgcagatc
     2101 ggcgtgggcg tcgatctgga cgacggtgat ctcgcccgcc tcgccggccc aggcgcgaaa
     2161 ggcggcgttg ctgatcgagt gctccccgcc gaccagcacg cgcaatcgtc cgtcgtgcgc
     2221 gcgatagcgt cgctcgagac gctcgaccat cgccgcgggc ggcaagccgg cgagatcgtc
     2281 ggcgtcgacc caggcgatcg ccagccgctc ggccgggttg cccccgctga tgcggtcgtc
     2341 gaactcgatc tggtggtcga ggcagtcgcg cacggccgcc ggaccgcggt cggcgccctt
     2401 gccgaaggac gaggtcccgt cgtagccggc gccgatcagc accacgtcgg cccgcgcggg
     2461 atcggcgcag ttgatgatcg agtcggccac ctcagtcgta cagccggccc cagcggcgcg
     2521 gcgcgcgatc gcgccaggcg ccggtgtggt aggcgtggct gccgagcagc gggatgacgc
     2581 tcgtcgcctc ggcgtagacc atctgctcgt aggtcacgtc gaccttgccc cacgagcagg
     2641 cctccttcaa cgtcgagctc gagcaggcgc cgtcgcgggc gtcggccacc gtgatctgga
     2701 tcgcgtactt gtgcatcggc acttcgcggc cgagcagctc ggcgcagatc accgtgtcct
     2761 gcaggaagtt cttcggcact ccaccgccga cgatcagcat cccggtcgtc tcggcggcga
     2821 tcttcatctc ggtcagctcg cggaagtcgc gaaccgagtc gatcgcgacg tgcgccgcgg
     2881 ggcgctcgtg ctggtggatc accagcccga accccgcgct cgagtcggag aaggcgggac
     2941 agaagatcgg cacctcgcgc cggtaggcgg tctcgaccag cgacccggat ttcttcgcgt
     3001 tgccggcggc gagccagcgt cccagctcac ggatgaaccc cctcgacgag tagacgccgg
     3061 gagcgagcga gtcggcgatc tccttgatct tcgcgtcgca cgcctgcagc tcgttctcgt
     3121 cgatgtaggt gtcgtagatc cggtcgatgt acagctcgcg caacgtgcgg tcgtcggcgg
     3181 tcgtcgagcc gcggtagtgg cgaaagccga gggcctcgaa gaaatccatg tcgatgatgc
     3241 tggcgccggt cgccaccacc gcgtcgatca tcccgtgttc gaccatgtcg acgtagacct
     3301 gcatgcagcc gccggcactg gtgctgccgg cgagcgacag cacgatgctg caatccgggt
     3361 cttcgagcat ctgctggaag atctcgcagg cgcgtccgag atcgcgcgcg gtgaacgaca
     3421 tcttccgcat cgcgcgcacg atctcggtgg cgtcgaagct cttgatgtcg atgtgctcca
     3481 ccggctcgct cagcagcttg cggcgttcga gtttgtcggt tttggtttcc atgccgtgct
     3541 ccacgggacg tcgcttcgtc cgatcgatcg atgtcgcgtc gaggatacgc caccgtcccg
     3601 ccacggtcca ccgcgccgcg aacggggtgc ggcgggcccg gtgttggttc ccccggcgcg
     3661 ccacggttat cctgctcccc atcggcgtcc gcgagccggc ggaccggagc ggcgcacgag
     3721 gggatcgatg agcgacgact acatccacgg gttcgcaccg gcggagcagc ggcggctgac
     3781 gctgatgcag gggatcctca acgacgagga gctgcgtcac ctcgacctcg acggcgtgcg
     3841 ccgcatcctc gacgtcggct cgggtctcgg tcagatgacg cgcgcgctgg cccgtgccgc
     3901 cggaccggcg gcgacggtcg tcggcgtcga gcgcgacgag cgccagctcg aggaggcggt
     3961 gcgtcaggcc gagggggcgg acgaggcctc gcgcgtcgag ttccgccagg gccgcgccga
     4021 gcgccttcca ctgacgcccg gcgagcgcgg gtcgttcgac ctggcgcact gtcggttcct
     4081 gctcgagcac gtgaccgacc ccctggccgt cgtgcgcgag atggtcggcg cggtgcgagc
     4141 gggcggccgg atcgtgttga tcgacgatga ccacgagctg ttgcggctgt ggcccgtggc
     4201 ggactacgtc gagcaggtgt ggcggatcta ctgggagagc taccgcgatc gcggccacga
     4261 cccgctgatc ggccgccggc tcgccggtct gctgcgcgag gccggggcgc gcccgacgcg
     4321 cgtgacctcc gtgttctacg gcgcggtgcg cggcatgcgg ctgttcgacc cggtggtcga
     4381 caacctggcc agtgtgctgg gcggcgcggc cgagcggctc gacgaggagg gcgtgctcgc
     4441 gcgcccggcg atggagcagg cgctggccga actcgacgag tggcgccacc aggcgagcgc
     4501 caccctgtgg tactcgctgc cgctggccga gggcacggtc ggctagacgc ggtccgcggt
     4561 cgctactcgc gcggtgcggc ggcgggatcg gcgaccgccg taccgagtcg acggctcgtc
     4621 gcctggtaca gcccggtcag cccggaggcc acaccgaact cgagcgcctc gtccggcggc
     4681 agaccgccga gccaggcggc gcgcagcgcg agcatcgcgc cgatccggtt cccgctcgcg
     4741 caatgcagca gcaccggacc ccgctcgaga ccgctcgaca gcgccgagtc gagctgctcg
     4801 acgcgctcgc gggtcagatc ccgctcgccg gccaccggga tccttacgta gcgcaggccg
     4861 aggcgctcga cggtttcggc ttcccactcg aacccggtct caccgtccga gcgcaggttg
     4921 atcaccgtcc ggaagccggc gcgcgcggcc gcctcgatct ggtcgggtgt cggctggccg
     4981 ccgctcagca cctcggccag cgggatgcgg gcgttcggga tcggcagcag cgcgctcggg
     5041 cggggggtgg cgtccggcgg ctcaccgccc tcggcacgcg cggtgggcca ccccgtcgcg
     5101 accagcagga tcgcgaccgc gagccacgat ctgccgtcgc ggatccgcat cgcttcatta
     5161 tagaagcggg cggcgccggg cgagagcccg gtcgatcagc cggcgacggc gacccgcggc
     5221 gcgagcacca gcacctcgtc gagccgggcg tcgagatcgg cttcggtgaa gcagaccggc
     5281 ggcgcggcga ccggggcgat cgccgggcgg gaggccaccc gctccggacg gcgcggcggg
     5341 ccggcggcga ccccgcccga gcgcttcaac agcgcctgga cgatctcgag cagctcgccg
     5401 tcctggtacg gcttctcgat gtaggcgtcg cagtcggtct tcatcagcgt cacgtgctgg
     5461 cgcggcccgg tgaggatcac gaccggcagg tcggccgact ccgcggtgtg cttgatctgg
     5521 ccgcacacgt cgagaccgcc gatccccggc agcaggtcct gcacgatcgt cacggccggg
     5581 cgcagccgtt gcgcctcggc gacacccgac cgcccgtcga gcgcgagctc gacccgcaca
     5641 ccggcgggcc cgagggtgcg gcgaatccgg ccgatgctgt tcgggtcgta gtcgactagc
     5701 aacacggtct tggtcatcgc gtcgtggcct cctccacaac cgctacgatc ggccggcgaa
     5761 aagacaacgg aggatgtcgt aatccgcgcc acccgccgcc ccgacggatg ccgacaacgc
     5821 cgcgcgggtc gcccgtggcg ggcgatcagg ggttcgcgcc ctcgtcgatc catcgttcga
     5881 cgaggcggat cttcgagtcg ctgagcggtc caccgacgag cggcatcggg tcgcccagga
     5941 tccgcagatc gttcgtgatc ttcatgtaca cgtacgagtc ggcggcgttc cccggctcga
     6001 cacgcatgta gttgttgatc tcggccgcct ggacgttgac caggttggcg taggcgctgc
     6061 cggcgctgag gtcgagccct tgctcggggt cggggccgat gtggcagtcg gtcaccgcgc
     6121 agctcggcga gaagacctgc tgctgcacgg tggccaacgt gatcggaccg gagagcgtct
     6181 ggctcggcga gtcgctgccg ccgcagccga ccgcaagcgt acagataccg atcgacaaca
     6241 tcgcgcgctt cacgccgtcg tctccgtcga cccctgttcc cgtcgaaagg taggtcctcg
     6301 ccggcccggc gacaaacgcg cgccgggcgc cgcgccggcc ggatgagacg gccgactgac
     6361 gcattcgtcg cgatcgttac gaagccgtag cgagccggtc gcgcgcgtac cgcccgccgg
     6421 gcgataccac gccaaccgcg acgtccgcgg gtggtatcgc ccttgcgtga gaggccgcgg
     6481 aggcgtccca cggacgccgg aggtgacgcc catgtccccc agccgaaccc tccgcctgac
     6541 gctggccctg ctgctcgtga gcgcaccggt cgccgcggac agcgggctcg cggtcccgcc
     6601 ctccgaagcc tcgcgcttcc tgtcgcaggc gactttcggc gccaactggg aggagatcga
     6661 gcgcacctcc gaggtcgggt tcgaagcgtg gttggccgag cagttcgtcc ggccggtcgg
     6721 ccggcaccag ccaccgctcg aagagctcgc ccggctgggg gtcgaggtcg agaaccagca
     6781 tcgccgctgg gtctggtggg agcgcgcgat gcaggggccc gacccgctcc ggcagcgcgt
     6841 cgcgttcgcg ctgagcgaga tcttcgtcgt gtcggacaac accgaagcga tccgcgacac
     6901 cccgatcggg atggcgaact actacgacat gctgctcgac aacgcgttcg gcaacttccg
     6961 cgagctgctg ctcgacgtct cgctgcaccc gatcatgggc gtctacctca gccacctgag
     7021 gaacgatcgc tccgacccgt cgggcggtcg cttccccgac gagaactacg cgcgcgaggt
     7081 gatgcagctg ttctcggtcg ggttgttcga gctgcgaccg gacggcacgc agtacaccga
     7141 cggcgacggc gagccggtgc cgacctacga caactcggac atcaccgagt tcgccaagat
     7201 cttcaccggg ctcgcgttcg acagcccggc gcccgacttc ggcaacggca ccccggtctg
     7261 gacccgcccg atgcggatgt accaggagca ccacgagccg gggccgaagt acctgctgcg
     7321 cggcggcttc gtgccgccgg gccagaccgg catgcacgac gtcgccgacg cgatcgacaa
     7381 cctgttccgg caccccaacg tcggcccgtt catcgggcgt cggctgatcc agcgcctcgt
     7441 cacgtcgaat cccagcccgg actacatccg ccgcgtcagc gccgccttcg acgacaacgg
     7501 cgcgggcgtg cgcggcgaca tgcgggcgct cgtcaccgcg atcctgaccg atcccgaagc
     7561 gcggcagatc ccgaaccgca ccgagaccac gcgcggccgc ctgcgcgagt cgtacctcag
     7621 gcgggtccac ctggcacgcg cgttcgacgc ggccaatctg gtcggcagct acccgatcac
     7681 cgacggcggc gcgccgacca cgttcggcca gctgccgatc agctcgccga cggtcttcaa
     7741 cttcttcctg cccgaccacc agccgaccgg cgagctcgcc gacgccggtc tgtacgcgcc
     7801 cgagttccag atcatgaccg ccgtcaccgc gatcgccagc gccaacgcgc tgcagcgtca
     7861 ggtcgagggg ctgatgaacg acgacgacga cccgatgaac gaggtgcggc tcgacctcag
     7921 caacgagatc gcgatcgccg ccaacagccg cgcgctggtc gaccgtctcg acctgctgct
     7981 gatgtacggc gacatgagcg tgccgatgaa ggagatcctg gtccacgcgc tcgaccagct
     8041 gccggacccg atcgaacggg tcgagatggc gatccacctg atctcgatcg cccccgaata
     8101 cgcggtgcag aagtagccgc cggagggaac ggatgaacga cgagcaaaac ggcgacgagc
     8161 cgaacggcgt gagccgacgc aggttcctcg gccaggcgag ctgcgccgcg gtcggcacga
     8221 ccgcgatctt ctcgaccgtg ctcgacatga gcatgatcaa cgtgctggcc cagaccacgc
     8281 cgggcgacta ccgcgcgatg gtctgcctgt tcttcggcgg cggcatcgat tcgttcaacg
     8341 tcgtcgtgcc gcgcggccag gccgagtacg acgagtacgc gcaggtgcgg cgcgacctcg
     8401 cgctgccgca ggacgtgctg ctgccgatca cgccggtcac gcccgacggg cgcgagtacg
     8461 gattgcatcc caacgtgccc gagctgcagg cgttgttcgg ccagggcgat ctgg
//
LOCUS       DASPGN010000621         6467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_936290, whole genome shotgun sequence.
ACCESSION   DASPGN010000621 DASPGN010000000
VERSION     DASPGN010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936290"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VD788_18030"
     CDS             complement(<1..384)
                     /locus_tag="VD788_18030"
                     /inference="COORDINATES: protein motif:HMM:NF013396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HXV78215.1"
                     /translation="MFRKARRVHMVGIGGSGMSGIAEVLVNMGYPVSGSDLVINEATR
                     RLKRLGARVQRGHRPQNVHDADVVVVSSAVQKDNAEVIEARRMKIPVIPRAEMLAELM
                     RMKYGVAVGGAHGKTTTTALIAEVLT"
     gene            complement(374..1510)
                     /gene="murG"
                     /locus_tag="VD788_18035"
     CDS             complement(374..1510)
                     /gene="murG"
                     /locus_tag="VD788_18035"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HXV78216.1"
                     /translation="MWNEENQFSGRPQIVFAGGGTAGHVFPALAVADEMRRRHPQASI
                     RFIGAKRGLEQRLVPRAGYRLRSLGVAGIQGRGPVGKLVAGASAGWAVVRCLLWLLAS
                     KPDLVIGVGGYASGPAVLAATLLRRKTMVMEQNHYPGATNRWLAPRVDAVCVPSAAAR
                     LRMGDRGIVTGNPVRREFAEIGDPPLGAVPSVLVFGGSRGARSINDAMIGAAGPLARH
                     PTPPRLVVQTGRDDAERVGRAFADYPRDLCSVVEFIDDMPGRLASADLVVCRAGASTL
                     SELAVAGRASILVPYPFAADDHQRHNAESMRDAGAARMIVDAELDGERLARALLELCA
                     DHERLRAMGRAARSLGRPRATVAIADVADRLMRGLDPERAADVP"
     gene            complement(1512..2603)
                     /gene="ftsW"
                     /locus_tag="VD788_18040"
     CDS             complement(1512..2603)
                     /gene="ftsW"
                     /locus_tag="VD788_18040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HXV78217.1"
                     /translation="MPRTLAHDHWLFFSASLLALAGLFMVGSASTHVALASTDDPSAY
                     LWKHLVHLAIGFVGMILAMSFDYRRLADGKKILILFGITLGLLILVLAMPESGGAHRW
                     IELGPFRFQPSEMAKLVTVLFMAYVLAKKEESVNDLWSVPIPTMLFVGTLAFLVVIEP
                     DLGSAVMLVMVAGVMVFAAGLGWRYVAVGCGLGATVFGLAVIAEPYRLRRILGYLNPT
                     ADLQGLNFQLHQSLIALGHGGLTGTGLGQGQQKWYYLPAAHTDFIFSVVGEEVGLLGT
                     VLLLLAFLLIFWRGLRAAVRAPDRFGSYLALGITNLIVLQALVNMCVCLGLLPTKGLP
                     LPFISYGGSALLVSMTAMGLLLNVSKHSN"
     gene            complement(2596..4014)
                     /gene="murD"
                     /locus_tag="VD788_18045"
     CDS             complement(2596..4014)
                     /gene="murD"
                     /locus_tag="VD788_18045"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXV78218.1"
                     /translation="MNETTMKRQPFATRNPDLRNRRVLVLGMGKSGLAAARLAAAHGA
                     RVTIADRKPAERLGDAIERARRTGAQVHPGGHPASLVDRADLVVVSPGVPGGVEPLVA
                     ARRAGVPVWSEIELAARFCRGRVVGITGSNGKSTVTAMTGAILRRAGVPGGTGGNLDV
                     PFCDLLEHDADNAVHAVELSSFQLEHVESFSPGVAAVLNLTPDHLDRYPDLDAYAAAK
                     ARLLEVQQPGAAAILNADDGSAERFRPAVRGRLYLFSVRRPVDQGAFVRDSRLVLRTA
                     AGEETLFARDELPLPGEHNVANALAAALAARLVGCRLAAVAAALRDYRALPHRLEHVG
                     RVGGVEFYNDSKATNPDSTMRAIEAFEPGRVVLILGGRDKGADWDALVDGLVARRVRV
                     MLVGEAAAMLSTRLASRVPVIDCGTIPRAVERGFADAGPGDVVLLSPGCASFDQFDHF
                     IARGDAFRQAVERLEPPEVPDA"
     gene            complement(4011..5096)
                     /gene="mraY"
                     /locus_tag="VD788_18050"
     CDS             complement(4011..5096)
                     /gene="mraY"
                     /locus_tag="VD788_18050"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXV78219.1"
                     /translation="MFFHLLYSLHDEFGAFNVFRYITFRTAMAAMTALVVSLLLGPSL
                     IARLRHSQIGQAIREEGPSSHQAKAGTPTMGGLMIIIAAVLPTVLWADLTNVFVWIAI
                     VATLAFGTIGFIDDYLKVSRRRNLGLRASTKFSLQTIVALALGAGLVWLAREGQFSTA
                     LSVPFFKNLNPDLGYAYPLFVVLVITGAANAVNLTDGLDGLAIGSLLVAWVTFVLLTY
                     AAGNAIVADYLGIPNVKGTGELTVFCGAVVGASLGFLWFNCHPAEVFMGDVGSMAMGG
                     ALGTVALMIHQEVLLILVGGLFVIEAASVILQVGSFKLRGKRIFRMSPLHHHFELIGW
                     SESKVVIRFWIMAIIFGLLSLATLKLR"
     gene            complement(5098..>6467)
                     /gene="murF"
                     /locus_tag="VD788_18055"
     CDS             complement(5098..>6467)
                     /gene="murF"
                     /locus_tag="VD788_18055"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HXV78220.1"
                     /translation="LVQATGGELLRGAPETRVDSFVIDTRRLETRGVFFALKGERTDG
                     HRFLGQAAERGAAAVIQQQPEADAPAPAGLILVADVAVALAACGSWVRRMLRPKKWIA
                     VTGSNGKTTTKELIAEALTAGYRVHRTPGNLNNHLGVPLSLLACPADAEMVVLEMGMS
                     GYGEIAALAGMADPDVALITNVRAAHLAAFDSLDDIAAAKGELFAVLRDEATAVVNLD
                     DTLVRVQAARHVGPRVTFGQHPAADVQMEEVHSRFVPGSEVVFRHAGRRYRVSLRIGG
                     AHAAFNAVAAAAVVVAAGADVGAGIERMERLEAGPGRGRVHRLQRGMMLVDDSYNSSP
                     AALASVLETLRVSQTEGRRVLVMGDMLELGRMEGALHREAGKRAAAAGVKLLFAVGAA
                     SRAAAESARRAGVPEVHHHADSRKAAESIVEFLHPGDLIVVKGSRAVGLERVVQALLA
                     EYGEN"
BASE COUNT         1113 a         2284 c         2174 g          896 t
ORIGIN      
        1 cgtcagcacc tcggcgatca acgcggtcgt cgtcgtcttg ccgtgagcgc cgccgacggc
       61 cacgccgtac ttcattcgca tcagttcggc gagcatctcg gcccgcggga tgaccgggat
      121 cttcatccgg cgcgcctcga tcacctcggc gttgtctttt tgcaccgccg acgagacgac
      181 gacgacgtcg gcatcgtgca cgttttgcgg gcggtggccg cgttggacgc gagcgcccag
      241 acgcttcaac cggcgggtcg cctcgttgat caccaggtcg gagccggaga ccgggtagcc
      301 catgttgacc agtacctcgg cgatgccgct cataccggag ccgccgatgc cgaccatgtg
      361 cacccgtcgc gccttacgga acatcggccg ctcgctcggg atcgagaccc cgcatcagac
      421 ggtcggcgac gtcggcgatc gcgacggtcg cccgcggtcg gccgagggac cgcgccgctc
      481 ggcccatcgc ccgcaaccgc tcgtgatcgg cgcacagctc gagcaacgcg cgagccagcc
      541 gctcgccgtc cagctcggca tcgacgatca tcctcgccgc ccccgcgtcg cgcatcgact
      601 cggcgttgtg tcgctggtgg tcgtcggcgg cgaacgggta gggcacgaga atcgacgcgc
      661 ggccggcaac cgcgagctcg gacagcgtcg aggccccggc gcggcaaacg acgagatcgg
      721 ccgacgccag ccgtccgggc atgtcgtcga tgaactcgac caccgagcag agatcgcggg
      781 ggtagtcggc gaaggcgcgg ccgacgcgct ccgcgtcgtc ccggccggtt tgcacgacca
      841 gccgcggcgg cgtcgggtga cgcgcgagcg ggccggcggc gccgatcatc gcgtcgttga
      901 tcgatcgcgc cccgcggctg ccgccgaaga ccagcaccga cggaacggcg cccagcggtg
      961 gatcgccgat ctcggcgaac tcgcgccgca ccgggttgcc cgtgacgata ccgcggtcac
     1021 ccatgcgaag cctggcggcc gcggacggaa cgcagaccgc gtcgacgcgt ggcgcgagcc
     1081 agcggttggt ggcgcccgga tagtggtttt gttccatcac catcgtcttc cgcctcaaca
     1141 gcgtcgccgc cagtacggcc ggccccgacg cgtagccgcc gacgccgatg acgaggtccg
     1201 gctttgaagc gagcagccag agcaggcacc gcacgaccgc ccagccggcc gacgcacccg
     1261 cgaccagctt tcccacgggg ccgcgaccct ggatccctgc gacgcccagc gaccgcagcc
     1321 ggtaaccggc gcgtggcacg agacgctgtt ccaggcctcg tttggccccg atgaaccgaa
     1381 tcgacgcctg cggatgccgg cgacgcatct cgtcggccac ggccagagcg gggaacacgt
     1441 ggccagcggt acctccaccg gcgaacacga tctgcggccg gccgctgaac tggttttctt
     1501 cattccacac gtcagttgga gtgtttcgaa acgttgagca gcaaccccat cgccgtcatc
     1561 gatacgagca gcgccgagcc gccgtagctg atgaacggca gcggcagacc cttggtcggc
     1621 aacaacccga gacatacgca catgttgacc agcgcctgca gcacgatcag gttcgtgatg
     1681 ccgagcgcca ggtaggagcc gaatcgatcg ggagcccgca ccgccgcgcg cagcccgcgc
     1741 cagaagatca gcaggaacgc caacagcagc aggacggtgc cgagcagtcc gacctcctcg
     1801 ccgacgaccg agaagatgaa gtcggtgtgc gccgcgggta ggtagtacca cttctgctgg
     1861 ccctggccga gaccggtccc ggtcagaccg ccgtgaccca gcgcgatcag cgattgatgg
     1921 agttggaagt tgagcccctg caggtccgcg gtcgggttga ggtagccgag gatacgacgc
     1981 agccggtacg gttcggcgat cacggccagc ccgaacacgg ttgcgccgag cccgcagccg
     2041 accgcgacgt agcgccagcc gaggcccgcg gcaaagacca tcaccccggc gaccatcacc
     2101 agcatcaccg ccgagccgag gtcgggttcg atgacgacga ggaaggccag cgtgccgacg
     2161 aacagcatgg tcgggatcgg cacggaccac aggtcgttga ccgattcttc cttcttggcc
     2221 aagacgtagg ccatgaacag caccgtcacg agcttggcca tctcggacgg ctggaagcga
     2281 aacggcccga gctcgatcca gcgatgcgcg ccgccggact ccggcatcgc cagcacgagg
     2341 atcaacagac cgagcgtgat gccgaacaga atcaggatct tcttgccgtc ggccaatcgg
     2401 cgatagtcga acgacattgc gaggatcatg ccgacgaacc cgatcgccag gtgaacgagg
     2461 tgcttccaca gataggccga ggggtcgtcg gtcgacgcca gcgccacgtg cgtcgaggcg
     2521 ctgccgacca tgaacaggcc ggccaacgcc agcagtgagg cggagaagaa cagccagtga
     2581 tcatgtgcca gcgtcctagg catccggcac ctccggtggt tcgagccgct cgaccgcctg
     2641 gcggaacgcg tcgccgcgcg cgatgaagtg gtcgaactgg tcgaacgagg cgcaacccgg
     2701 cgagagcagc acgacgtctc ccggtccggc gtcggcgaaa ccgcgttcga ccgcgcgcgg
     2761 gatcgtcccg caatcgatca ccggcacgcg gctcgcgaga cgggtgctga gcatcgccgc
     2821 cgcctcgccg acgagcatca cgcgcacgcg gcgagcgacg agcccgtcga ccagcgcgtc
     2881 ccaatccgcg cccttgtcgc gcccaccgag gatcaacacg actcgaccgg gctcgaacgc
     2941 ctcgatcgcg cgcatggtcg agtccggatt ggtcgccttg gagtcgttgt agaactcgac
     3001 gccgccgacc cggccgacgt gttccagccg gtgcggcaac gcgcgatagt cgcgcagcgc
     3061 ggcggcgacc gccgccagac ggcaaccgac cagtcgcgcg gcgagcgcgg cggccagcgc
     3121 gttggccacg ttgtgctcgc cgggcagcgg cagctcgtcg cgtgcgaaca gcgtctcctc
     3181 gccggccgcc gtgcggagca ccagccggct gtcgcggacg aacgctccct gatcgaccgg
     3241 ccgacgtacg gagaacaggt acaggcggcc gcggaccgcc gggcggaacc gctcggccga
     3301 cccgtcgtcg gcgttgagga tcgccgcggc gccgggctgt tgcacctcga gcagacgcgc
     3361 tttggccgcg gcgtaggcgt ccagatccgg gtagcggtcg aggtggtccg gcgtcaggtt
     3421 caacaccgcg gcgaccccgg ggctgaacga ctcgacgtgc tcgagttgga acgaggaaag
     3481 ctcgacggcg tgcaccgcgt tgtcggcgtc gtgctcgagc aggtcgcaga atggaacatc
     3541 gagattgccg ccggttccac ccgggacacc ggcccggcgc aggatcgcgc cggtcatcgc
     3601 ggtgaccgtg ctcttgccgt tcgaccccgt gataccgacc acgcgcccgc ggcagaagcg
     3661 cgcggccagc tcgatctcgc tccagaccgg cacgccggcg cgacgcgcgg cgaccagcgg
     3721 ctcgacaccg ccgggcacgc ccgggctgac cacgacgagg tcggcccggt cgacgagtga
     3781 tgcgggatgc ccgcccgggt gcacctgagc accggtgcgg cgcgcgcgct cgatggcgtc
     3841 gccgagacgc tcggccggct tccgatcggc gatggtgaca cgcgcgccgt gcgccgcggc
     3901 cagacgtgcc gcagccaagc cgctcttgcc cattccgagc accagcaccc gacggttgcg
     3961 caggtccgga ttgcgggttg cgaacggctg gcgtttcatc gtcgtttcgt tcatctcagt
     4021 ttgagtgtcg ctagcgagag cagaccgaag atgatcgcca tgatccagaa gcggatgacc
     4081 accttcgatt cgctccagcc gatcagctcg aagtgatgat gcagcggcga catgcggaag
     4141 atgcgcttgc cgcggagctt gaacgagccc acttgcagga tgaccgaggc ggcctcgatg
     4201 acgaacagcc cgccgaccag gatcaacagc acctcctgat ggatcatcaa cgcgacggtt
     4261 cccagcgcac ctcccatcgc catcgagccg acgtcgccca tgaatacctc tgccgggtgg
     4321 cagttgaacc agaggaagcc gaggctggcg ccgacgacgg cgccgcagaa aaccgtcaat
     4381 tcgccggtcc ccttgacgtt cggtatgccg aggtagtcgg cgacgatcgc gttgcccgcc
     4441 gcataggtca gcaagacgaa ggtaacccag gcgaccagca gtgagccgat cgccagaccg
     4501 tcgagcccgt cggtcaggtt gaccgcgttc gccgctccgg tgatcaccag cacgacgaac
     4561 agcggatagg cgtaacccag gtcggggttg agattcttga agaacggaac tgacaaggcc
     4621 gtcgagaact ggccctcgcg cgcgagccaa accagaccgg cgccgagtgc cagcgcgacg
     4681 atcgtttgca gcgagaactt ggtcgaggcg cgcaggccga gattgcgccg ccgtgagacc
     4741 ttcaggtagt cgtcgatgaa gccgatcgtg ccgaaagcca gcgtggcgac gatcgcgatc
     4801 cagacgaaga cgttggtcag gtcggcccac agcacggtcg gcagcaccgc ggcgatgatg
     4861 atcatcagac cgcccatcgt cggtgtgccg gccttggcct ggtgcgacga cgggccctcc
     4921 tcgcggatcg cctgaccgat ctgcgagtga cgcagccgcg cgatcaacga cggcccgagc
     4981 agcaacgaca cgaccagcgc cgtcatcgcc gccatcgcgg tgcggaaggt gatgtagcgg
     5041 aagacgttga acgccccgaa ctcgtcgtgc agcgagtaga gaagatggaa gaacacctca
     5101 gttctccccg tattcggcca gcagcgcctg gaccacccgc tcgagaccga cggcccgcga
     5161 acccttgacc acgatcaggt cgccggggtg gaggaactcg acgatcgact ccgccgcttt
     5221 gcgcgagtcg gcgtggtggt gcacttcggg cacgccggcc cgccgggccg actcggcggc
     5281 ggcgcgcgac gccgcgccga cggcgaacag caacttgacg ccggcggcag cggcgcgctt
     5341 gcccgcctcg cggtgcaacg cgccctccat tcggcccaac tccagcatgt cgcccatcac
     5401 cagcacccgc cgcccctcgg tctggctgac gcgcagcgtt tcgagcaccg atgccagtgc
     5461 ggccgggctg ctgttgtagc tgtcgtcgac cagcatcatc ccccgttgca aacggtggac
     5521 ccgcccccgc cccggtccgg cctcgagccg ctccatccgc tcgattccgg ccccgacgtc
     5581 ggcgccggcg gccaccacga ctgcggccgc ggcgacggcg ttgaacgccg catgcgcgcc
     5641 gccgatccgc agcgagacgc ggtagcgcct gccggcgtgg cggaaaacga cctcggaacc
     5701 cgggacgaag cggctgtgca cttcctccat ctggacgtcg gccgccggat gctgtccgaa
     5761 cgtgacgcgc ggacccacat gacgggccgc ctggacacga accagcgtgt cgtccaggtt
     5821 gaccaccgcg gtcgcctcgt cacgcagcac ggcgaacagc tcgcccttgg ccgccgcgat
     5881 gtcgtcgagc gaatcgaacg cggcgaggtg cgccgcgcgg acgttggtga tcagggcgac
     5941 gtcaggatcc gccatcccgg ccagtgcggc gatctcgccg taaccgctca tgcccatctc
     6001 gagcacgacc atctccgcgt cggccgggca ggccagcagg ctgagcggca ctccgaggtg
     6061 gttgttcaag ttgcccggcg tgcggtgcac gcggtaaccg gccgtcaacg cctccgcgat
     6121 caactccttg gtcgtcgtct taccgttgct gccggtgacc gcgatccact tcttcggccg
     6181 cagcatccgc cggacccagg aaccgcaggc ggcgagcgcc accgcgacgt cggcgaccag
     6241 gatcaaaccc gcgggcgcgg gcgcgtccgc ctccggctgt tgctggatga cggccgccgc
     6301 gccgcgttcg gctgcctgac ccaggaagcg gtgaccgtcc gtccgttcgc ccttgagcgc
     6361 gaagaacacg ccgcgtgtct ccagccggcg ggtgtcgatg acgaacgaat cgaccctggt
     6421 ttccggtgct ccgcggagca gctctcctcc ggtggcctga acgaggt
//
LOCUS       DASPGN010000622        15249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Candidatus Polarisedimenticolaceae bacterium isolate
            SMAG_U16452 k141_585313, whole genome shotgun sequence.
ACCESSION   DASPGN010000622 DASPGN010000000
VERSION     DASPGN010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37935447
            Sequence Read Archive: SRR25158314
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Polarisedimenticolaceae bacterium (soil metagenome)
  ORGANISM  Candidatus Polarisedimenticolaceae bacterium
            Bacteria; Pseudomonadati; Acidobacteriota; Candidatus
            Polarisedimenticolia; Candidatus Polarisedimenticolales; Candidatus
            Polarisedimenticolaceae.
REFERENCE   1  (bases 1 to 15249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/08/2024 17:28:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,627
            CDSs (total)                      :: 3,585
            Genes (coding)                    :: 3,565
            CDSs (with protein)               :: 3,565
            Genes (RNA)                       :: 42
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 21
            CDSs (without protein)            :: 20
            Pseudo Genes (ambiguous residues) :: 0 of 21
            Pseudo Genes (frameshifted)       :: 1 of 21
            Pseudo Genes (incomplete)         :: 18 of 21
            Pseudo Genes (internal stop)      :: 3 of 21
            Pseudo Genes (multiple problems)  :: 2 of 21
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15249
                     /organism="Candidatus Polarisedimenticolaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585313"
                     /isolate="SMAG_U16452"
                     /isolation_source="soil"
                     /db_xref="taxon:2910585"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.18 N 121.35 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1959)
                     /locus_tag="VD788_18060"
     CDS             complement(<1..1959)
                     /locus_tag="VD788_18060"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXV78221.1"
                     /translation="MRSRFGRAAAMVVRLALCLVAGTVFAGGAVEPKQLRLGVGSFDP
                     LTGELPLLPHLRLDAAPSHGYYLLQLTGERDDVVTSRLRELAVTPLDYVPDDAWVFRA
                     SPATIAALRAEPWVRWVGPLQPGWKLSPRLGEVPPRATIDLHHGEATEAASERLAALE
                     IDVLAVHRFGDTRRLTVAATRSQLERAAHIDAVSWIAPVAELTLRNDRSSWVVQSFEP
                     GVRSIWERGLHGEGQIIGHIDGPIYYKSCFFIDLFEEVPGNGHRKIVSYRGNGIGPEE
                     HGTHTAGTVAGDPSPVGGGVGSAGIAYRARLSHTDLSLIGTDPSILYGFLEQAHADGA
                     RVHTNSWGDDDNNSYTPLVRDLDLFAYEHEDSLLVFAGSNSPWARSPENAKNVLAVGA
                     SGNGADADAFCSGGSGPTIDGRRKPEIFAPGCGVVSALHRTLCDTVSGTGTSMAAPAI
                     AGAAALARQYFEQGWYPEGRPNPTDARPPSAALIKAVLLNSAANMLGVDGYPTDQEGW
                     GRVLLEDALYFAGDPRRLVVLGDVRNGLGLASGVSDHLALEVTGSSEPLELTLVYTEP
                     PAALMAQVATVNDLDLVVRSPGGRSYFGNVFDPEQGWSVAGGEADRLNNVEVIRIESP
                     APGRWSVRVTGASVNQGLQGYALIATGEV"
     gene            complement(2075..3457)
                     /locus_tag="VD788_18065"
     CDS             complement(2075..3457)
                     /locus_tag="VD788_18065"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXV78222.1"
                     /translation="MRRCRPNVGNALQSAARRRAPWRADARAITDRTRGAFPLKPGAR
                     AVWAWALFDFANSPFTTIVVTFVYSTYFTQAIVTDPIHGTVLWSRAVTLSAIVVAVVS
                     PVLGSVADVAGWRRPLLLGTTAVCVAATAALYEVLPGEVSRALALFVVANIAFELANV
                     FYNAVLPEIAAPQRIGRVSGLGWGLGYVGGLLSLVVALVTLVRPETPWFGFTTEQGQN
                     VRATTLLAALWFAVFSVPIFLWTGHPARPPAPARRAVARAVDDIRLTLRRIGAYRQIV
                     RLLIARLVYNDGLVTVFAFGAIYAAETFGFSLTEVLVFGIVLNVAAGIGSFAMGYADD
                     RLGGKRTILISLAGLIVATFVAVVAVSKAWLWVAGVLIGIFVGPNQSASRSLMARFIP
                     RGAESKFFGFYAFSGKLTAFAGPLLLGLLTQSTGSQRAGIAVVLLMFVVGFVLLLLVD
                     EDEGVAAAHR"
     gene            complement(3454..3951)
                     /locus_tag="VD788_18070"
     CDS             complement(3454..3951)
                     /locus_tag="VD788_18070"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HXV78223.1"
                     /translation="MDWDLVGTVMLYGVGLALVAFGLVGLVLPVVPGAPLIFLGIVSV
                     AWADGFERIGGWGIGICGALTLLTVMADYLAGAVGARKFGASRWGLAGALAGMVVGLF
                     FGLPGLLVGPIAGAVALEYFNDPDFRRAGRVGLGTLIGFVLGTAVRYALAALTLGVAL
                     LLYLF"
     gene            4078..4350
                     /locus_tag="VD788_18075"
     CDS             4078..4350
                     /locus_tag="VD788_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78224.1"
                     /translation="MSESEKPGSGEQFAEWKERYAAGDESAKELFRQISHIALLLAYD
                     LIDKLRWYESETIKLTDAEIDERIPHISQFLEQLQTAYDHFADVDL"
     gene            4398..5054
                     /locus_tag="VD788_18080"
     CDS             4398..5054
                     /locus_tag="VD788_18080"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HXV78225.1"
                     /translation="MGDERRIHQFDGKQLDVAWDGRLCIHAQECTRAKGELFVSGRQP
                     WGEPDRGDPDYVAEVVRRCPSGALTYRRGDGGEPESAAPRNTVIVANNGPLYVQGQLR
                     IDAAAPDMPGVAFRAALCRCGDSKNKPFCDNSHERAGFRDHGALGQTGEGFDAPGGPL
                     EIKRAPDGPLLLSGSFTIVSGSGREAWHGRKAALCRCGNSRNKPFCDGSHKTAGFHAD
                     "
     gene            5071..5718
                     /locus_tag="VD788_18085"
     CDS             5071..5718
                     /locus_tag="VD788_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78226.1"
                     /translation="MVSCSALSLSLSLSLGLACAAGASQAGSERECSSIRRDVPLAAT
                     VASSKAWANLRGQADSVRVESRRLLREAQVRSSAAAAPAGLCPTYCRPPSNPLIVLST
                     VPTDFLEGYDQADMCERLLETTRDEPLRFERRIFDSVDRLNDWVYDFTRGKGAQGEDL
                     YRRCAGKCSPQYGWTISQQGTSLAVDTEVICGHARDREVGRYTLRSSLRWICDAR"
     gene            5796..6536
                     /locus_tag="VD788_18090"
     CDS             5796..6536
                     /locus_tag="VD788_18090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78227.1"
                     /translation="MGTGRELSDTDRQRLTGALGVVSELITGPELDALRQVIEQLERK
                     LREELAELGGRLDGSLSDFKSEISRETDRLQLAVAAVEERLAAGIEQADRAVQGAAEA
                     VELELASARGAIADVDRRSAEDRQRLDRIAGQVDRALADAPDKLESRFEAVEAGLRSH
                     VDERVARLERAFQAIERKVSEQTQAAQRLSGLLTNLETVFSARQPTSQRSAADPDPTV
                     DPKSVDRDAPQMSSTELDNALNNLFHGS"
     gene            complement(6556..6825)
                     /locus_tag="VD788_18095"
     CDS             complement(6556..6825)
                     /locus_tag="VD788_18095"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HXV78228.1"
                     /translation="MSSRRFRTHSKVVWPGFSNTPSPRHILKVGRNDPCPCGSGKKYK
                     DCHVTDGSAYLEKLALDKGRERAREIRREARERGVPWYKRIFYRA"
     gene            6947..8470
                     /locus_tag="VD788_18100"
     CDS             6947..8470
                     /locus_tag="VD788_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78229.1"
                     /translation="MPAGRVLLALALIVVGIGAARWWPRTVDDAYITFRYADNLVAGH
                     GPVFNPGERVEGCSSPLWMTGSALLIAIGLDPVAGSKSGGLLASWVLVGLCYRTVRRS
                     GVSAAGAGVAALLLGSSLVLQIWSVAGMETNAYALLVFAGLVALTQGPLTRRAALVAS
                     LALVAAASTRPEGVGLWVLGSLVPWVAGERRLRAGSAYLLPGAILAGYVLWRLGFYGD
                     WWPNTYYAKTGGGVAMWKQGLDGLRLFVTHPAHAPWLVAAAIGGALALRSAGHVRQVL
                     VHGAAVGAHLLWIVSVGDDGLRVHRFYVPVLAPLATLSGFVFLPALERRFDRALARSA
                     MALAAIAIVVSLSTFHLRMVPEMRRALDYQRGNIALGRHLADHYPGESIAVAAAGAIP
                     YYSGAPTIDMYGLNDAHIARRPFAADQPGRMMKWDNAYVLARRPRFIVINRGYLAPGD
                     RLAERVLRDPRLLAVSALDRDLFRRVVSDGGYVPRLLELDGGAGFVVFERRPGRERR"
     gene            complement(8467..9825)
                     /locus_tag="VD788_18105"
     CDS             complement(8467..9825)
                     /locus_tag="VD788_18105"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXV78230.1"
                     /translation="MARRASSVLALVATGMAIAAVPAADTDAGNWPQFRGPGARGVAD
                     GHELPASWNVEDGTRVKWKTAIPGLGHSSPVVWGDAIFVTTAISGKPDPELKVGLYGN
                     IDAVEDDTEHRYRLYRLDRNTGSIVWERTVHQGVPEIKRHTKSSHANPSPTTDGKYVA
                     AFFGSEGLYVFDTEGKSVWNQDFGSLDSGYFQVPAAQWGFGSSPVIHDGRVIIQADVQ
                     QGSFLAALALATGDVIWKTVRQDVPTWSTPTVHVGESRSQVLVNGFKQIGGYDLDRGE
                     LLWSMRGTGDIPVPTPYVADGLVFLTSAHGPGSPIYAIRPTARGDVTLGEGQQTNDHV
                     VWSRRGGGSYMPTTLVYRGLLYVLRDNGVLTVYRALDGEQQYQQRLGTGSSGFTASLV
                     AGDGKVYATAETGEVYVVKAGAAFELSATNEMGEICMATPAISRGMIFYRTKDHLVAL
                     GD"
     gene            complement(9995..10459)
                     /locus_tag="VD788_18110"
     CDS             complement(9995..10459)
                     /locus_tag="VD788_18110"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78231.1"
                     /translation="MLNQRAETAFQHGAELLRRGRSRDAAAYLRAAIDLETERGASPG
                     QGEARYLSYYGLCLAQSTHKLREALQFCRRAAELEGYRPDVWWNLGRVALLTGRRGEA
                     YRALRLAAKLDPGHEGIATDLRKLGVRREPVLTFLDRRHPVNVFLGRLRPTV"
     gene            10929..11576
                     /locus_tag="VD788_18115"
     CDS             10929..11576
                     /locus_tag="VD788_18115"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXV78232.1"
                     /translation="MKRIRRDRPIRYLVASVLLTTTAAVSPVGSRASLAGAIDFADLE
                     RERVERAAILELVQRHRPADERCWQRHVAEVIYSESKAVGIDPLLTAAIVARESSFRS
                     RAESEAGALGLMQVRPFVAQDVALRRGVAWGGSETLHDPRLNVRLGTSYYGELLSRFD
                     GDVTLALAAYHRGPTRLSRQIREGSFSGAPYAGRVLHLYARLDAERQERLGQAGL"
     gene            complement(11591..11815)
                     /locus_tag="VD788_18120"
     CDS             complement(11591..11815)
                     /locus_tag="VD788_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXV78233.1"
                     /translation="METLGHEIECEAEYQLESSVRCAHCGQQFETVQVIRLLRKKVNF
                     TSSLPRRGYLVVCPHCQSAIPAVIGARGLA"
     gene            complement(11859..12998)
                     /gene="waaF"
                     /locus_tag="VD788_18125"
     CDS             complement(11859..12998)
                     /gene="waaF"
                     /locus_tag="VD788_18125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HXV78234.1"
                     /translation="MNPLALVQARIRTVVNDERTGRPVDARSGPAGGARLLVVGPSWI
                     GDMVMAQSLFLQLRRDDPSVTIDVVAPDWSRPILGRMPEIRRAIGLPVAHGRLGFAAR
                     WRIGRSLQGERYDRAIVMPKSWKSSLVPFFARVPVRTGYRGEQRFGLINDARPLDVSF
                     DRPMVRRYLALAPRAGGTVAEEIPRPALRVDRDNRGRVAARLGLASERPVVACVPGAE
                     YGPAKTWPAESFGELGRRLTASGVAVWILGGAAERETGERVRALAGPRARNLCGELRL
                     EDAVDLLSLTSTVVSNDTGLMHVAAAVGSHVVALYGSSSPVYTPPLTDRAEVLYLGLD
                     CSPCFERACPLGHHRCLRELSVDAVLAAVRRVSRTLRLDRGLSGT"
     gene            complement(12995..14614)
                     /locus_tag="VD788_18130"
     CDS             complement(12995..14614)
                     /locus_tag="VD788_18130"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXV78235.1"
                     /translation="MSPRRRQLLVLSLTALSICAACRSPRGETDRSLEPLPHPALAGS
                     DPVVLEQIEQTRGAVETTAADPRSSAADLGRAFGELGMTYHAYRLAAAAIPCYRNAER
                     LDPSEPRWPYYLGRLYRDAGAQDDAIAAFERSVADDPSYVPTLIELGRAESERGRYRD
                     GAAWFAAALDADPDSAAAWVGLGKIALAEGDYDHAVELLEAAVALAPSATAIRYPLGL
                     AYRGLGRIDRALPLLDGQGGVPPSVDDPWLDRVQRYRRDMLSHLDRGLRLSAEGRHAQ
                     ALAELRRAVEAAPEDVTARTTLGHGLAQAGDLDGARAELERALSLDPGSATAQFNLGT
                     VYAQTGDDERAVAHLRAALDIDPGLVPAHVNLGNALRRLGRFEQALAEYHWVIDRQPG
                     HAAARLNEGLVLIRLQRYAEALHRLDAAREAIPGDRNILQAVVRLLAAAPDDAVRDGR
                     RAERLARTLVAPDASFDVLEAVAMAAAENGDFDAAIDWQSRALAAIGDGAPAALIEEL
                     RGNMRGYRSRTACRRPWPDDGPVLAPGPLSR"
     gene            complement(14611..>15249)
                     /locus_tag="VD788_18135"
     CDS             complement(14611..>15249)
                     /locus_tag="VD788_18135"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HXV78236.1"
                     /translation="GLGTASRGFTGFGTAWVDIDNDGWLDLLLVNGAVKHIEELARAG
                     DPFPLDERDQVFLNLGDGSFAERSRVAGPAFELSEVGRGAAFGDLDNDGDTDVVVANN
                     SGPLRLLLNLVGQRNRWVGVRMTDPEGRYDVIGTEVSLRLEDGRSLVRRVRTAASYLS
                     SNDPRVLFGLGGASGVVSLRAVWPDGTIEDWEAPAIGRYTGIERGSGRVAAR"
BASE COUNT         2393 a         5369 c         5288 g         2199 t
ORIGIN      
        1 cacctcgccg gtggcgatca gggcgtagcc ttggagcccc tggttgaccg aggcgccggt
       61 gacccgcacg ctccacctgc cgggagccgg ggactcgatg cgaatgacct cgacgttatt
      121 cagtcgatcg gcctccccac cggcaaccga ccacccctgc tcagggtcga aaacgttgcc
      181 gaagtaactg cgtccgccag gcgaacggac gaccaggtcc agatcgttga cggtcgcgac
      241 ctgtgccatc aatgccgccg gcggctcggt gtagaccagc gtcagctcga gcggctccga
      301 gctgccggtc acctcgagtg ccaggtggtc ggagacgccg ctcgccagac cgagaccgtt
      361 gcgcacgtcg ccgagcacga cgagccggcg gggatcgccg gcgaaataga gcgcgtcctc
      421 gagcaggacc cggccccaac cctcctggtc ggtcggatag ccgtcgacgc cgagcatatt
      481 cgccgcggag ttgagcaaca ccgccttgat cagcgcggcg ctcggtggac gagcgtcggt
      541 cggattcggg cggccctccg ggtaccagcc ctgttcgaag tactggcgtg ccagcgccgc
      601 cgcacccgcg atcgccggcg cggccatcga ggtccccgtg cccgacacgg tgtcgcacag
      661 ggttcgatgg agcgcagaaa ccacgccgca gcccggtgcg aagatctcgg gtttgcgccg
      721 accgtcgatc gtcggcccgc tacctccgct gcagaacgcg tcggcgtcgg cgccgttgcc
      781 cgacgcgccg acggccagca cgttcttcgc gttctccggc gaacgggccc acggcgagtt
      841 gctgccggcg aagaccagca gcgaatcctc gtgctcgtag gcgaacaggt cgaggtcgcg
      901 caccagcggg gtatagctgt tgttgtcgtc gtcgccccac gagttcgtgt ggacacgcgc
      961 gccgtcggcg tgtgcttgct cgaggaaccc gtagaggatc gacggatcgg tcccgatcag
     1021 cgacagatcg gtgtgcgaca gtctcgcccg gtaggcgatt ccggccgatc cgaccccccc
     1081 gccaaccggc gacggatcgc cggccacggt gccggcggtg tgcgtcccgt gttcctcggg
     1141 gccgatgccg ttgcctcggt aggacacgat cttgcggtgt ccgtttcccg gaacctcctc
     1201 gaacaggtcg ataaagaagc acgacttgta gtagatcggg ccgtcgatgt ggccgatgat
     1261 ctggccctcg ccgtgcaacc ctcgctccca gatcgagcgt acccccggct cgaaactctg
     1321 tacgacccag ctcgaccggt cgttgcgcag cgtcagctcg gccacgggag cgatccacga
     1381 cacggcatcg atgtgggctg cacgctcgag ctgcgagcga gtcgccgcca ccgtcagccg
     1441 ccgggtatcg ccgaagcggt ggacggcgag cacgtcgatc tcgagtgcgg ccagccgttc
     1501 gctcgcggcc tcggtggcct cgccgtgatg aaggtcgatc gttgcccgcg gcgggacctc
     1561 acccaatcgc ggcgagagct tccagccggg ttgcagcggg cccacccaac gaacccacgg
     1621 ttccgctcgc agggcggcga tcgtcgccgg cgaggcacgg aagacccagg cgtcgtcggg
     1681 cacgtaatcg agcggcgtca cggcgagctc gcgcaggcgc gacgttacga cgtcgtcgcg
     1741 ctcgccggtc agttggagca agtagtagcc gtgcgacggg gccgcgtcga gacgcaggtg
     1801 aggcagcagc ggtagctcgc ccgtcaacgg atcgaacgag ccgacaccca accgcagctg
     1861 tttcggctcg acggctcctc ccgcgaagac cgtccccgcc acgaggcaca acgccaaccg
     1921 gacgaccatc gcggcggcac gaccgaatcg tgacctcatc gcccccccct gcgccacgac
     1981 cggtcgccga cggtcgcgag cgcccggatt ctacgcgagc gccggacgcc aggcaatcgc
     2041 cgaggctgga atggctcgat ccgccgggag cggctcagcg gtgtgccgcc gccacccctt
     2101 cgtcttcgtc gacgagcaac agcagcacga aaccgaccac gaacatcaac agcacgaccg
     2161 cgatgccggc gcgctgggag ccggtggact gcgtcagcag acccagcagc agggggccgg
     2221 cgaacgcggt cagcttgccg gaaaacgcat agaacccgaa gaacttgctc tcggcgccgc
     2281 gagggatgaa ccgggccatc aacgaccggc tcgccgattg gttgggaccg acgaagatgc
     2341 cgatcaacac tcccgccacc cacagccagg ccttcgacac ggcgacgacg gccacgaaag
     2401 tggcgacgat cagaccggcg agcgagatca agatcgttcg tttccctccc aaacgatcgt
     2461 ccgcgtatcc catcgcgaac gagccgatgc cggcggcgac gttgagcacg atgccgaaga
     2521 ccagtacctc ggtcagcgag aagccgaacg tttcggccgc gtagatcgcg ccgaaggcga
     2581 agacggtaac gaggccgtcg ttgtacacca ggcgcgcgat caacaggcgc acgatctggc
     2641 ggtaggcgcc gatccggcgc aaggtgagac ggatgtcgtc gacggcccgc gccaccgcgc
     2701 gccgcgccgg cgccggtggt cgggccgggt gacccgtcca gaggaagatc ggtacgctga
     2761 agacggcgaa ccaaagcgcg gcgagcagcg tcgtcgcgcg gacgttctgc ccctgttcgg
     2821 tggtgaaacc gaaccacggc gtctccggtc tgacgagcgt gaccagcgcg acgacgagcg
     2881 acagcaatcc accgacgtag ccgagccccc agcccagccc ggagacccgg ccgatccgct
     2941 gcggcgcggc gatctcgggc agcacggcgt tgtaaaacac gttcgccagc tcgaaggcga
     3001 tgttggcgac gacgaacagc gcgagcgccc gcgacacctc tcccggcagc acttcgtaca
     3061 gcgcggcggt ggccgccacg cagaccgccg tcgtgccgag caacagcggt cggcgccaac
     3121 cggccacgtc ggccaccgag cccagcacgg gcgagaccac ggcgacgacg atcgccgaca
     3181 acgtgaccgc ccgcgaccac aacaccgtgc cgtggatcgg gtcggtgacg atggcctggg
     3241 tgaagtatgt cgagtagacg aaggtgacca cgatcgtggt gaacggcgag ttcgcgaaat
     3301 cgaacagcgc ccaggcccaa accgcgcgag ctcccggctt cagtgggaac gccccgcggg
     3361 tccggtcggt gatcgctcgc gcatccgccc tccacggcgc tctccgccgg gccgccgatt
     3421 gtaacgcgtt cccgacattc ggccggcaac gcctcagaac aggtagagca ggagcgccac
     3481 gccgagcgtc aacgctgcga gagcgtagcg gaccgccgtg cccagcacga acccgatcag
     3541 cgtgccgagc ccgacccggc ccgccctgcg gaagtcgggg tcgttgaagt actcgagggc
     3601 cacggctccg gcgatcggcc cgacgagcaa gccgggcagg ccgaagaaca ggccgacgac
     3661 catcccggcc agcgcaccgg cgagccccca gcgggaggcg ccgaacttcc tggcgccgac
     3721 cgcgccggcc agataatcgg ccatcacggt cagcaacgtc agcgctccgc agatgccgat
     3781 tccccacccg ccgatcctct cgaacccgtc ggcccacgcg accgagacga ttccgagaaa
     3841 gatcaacggt gcgcccggga cgaccggcag cacgaggccg accagcccga aggcgacgag
     3901 cgccagaccg accccgtaca acatcaccgt cccgacgaga tcccagtcca tcccaccgcc
     3961 ccacgacgct cgcgttcgcg tcgaccgtat cccggttggt acaatccggg tcgatcgcgt
     4021 cgcaccgcaa ccttagcgtg gagggggccg ctcgcgcgag cgacccggga ggcctcgatg
     4081 tcggaaagcg aaaaaccggg ctccggcgag cagttcgcgg aatggaaaga gcgctacgcc
     4141 gccggggacg aatcggcgaa ggagctgttt cgacagatct cacacatcgc tctgctgttg
     4201 gcctacgacc tgatcgacaa gctgcgctgg tacgaaagcg agacgatcaa gctgacggat
     4261 gccgagatcg acgaacgaat cccgcacatc tcccagtttc tcgaacagtt gcaaaccgcc
     4321 tacgaccact tcgccgacgt cgacctttga gccgccggcg ccgggccgga ccgcgagtgg
     4381 atcatcggag gaacgacatg ggcgacgaac gccgaattca ccagttcgac ggcaagcagc
     4441 tcgacgtcgc gtgggacggc cggctgtgca tccacgcgca ggaatgcacg cgagccaagg
     4501 gcgagctgtt cgtcagcggc cgccaaccgt ggggcgagcc cgaccgcggc gacccagact
     4561 acgtggccga ggtcgtccgc cgctgcccga gcggcgcgct gacctaccgc cgcggcgacg
     4621 gcggggagcc cgagtcggcc gcaccgagga acacggtcat cgtggcgaac aacgggccgc
     4681 tgtacgtcca gggtcagctg cggatcgacg ctgcggcgcc ggacatgccg ggcgtcgcct
     4741 ttcgcgcggc actgtgtcgc tgcggcgatt cgaagaacaa gccgttctgc gacaactcgc
     4801 acgaacgggc cggattccgc gaccacggcg cgctgggtca gacgggggag gggttcgacg
     4861 cgccgggggg gccgctcgag atcaagcggg cgcccgacgg gccgttgctg ctgtcgggca
     4921 gcttcacgat cgtctcgggg tcggggcgtg aggcctggca cggtcgcaag gcggcgctct
     4981 gtcgctgcgg gaactcgcgg aacaagccgt tctgtgacgg aagtcacaag accgccggct
     5041 ttcatgcgga ttagcgggcg accgcgcacg atggtgtcgt gttccgcgct gtcgctgtcg
     5101 ctgtcgctgt cgctgggtct ggcgtgcgcc gccggcgcga gccaggccgg gtccgagcgg
     5161 gagtgctcga gcatccgtcg ggacgtcccg ctcgccgcga cggtcgcctc gtcgaaggcg
     5221 tgggccaacc tgcgggggca ggccgactcg gtccgcgtcg aaagccggcg gctgttgcgc
     5281 gaagcgcagg tgcgaagctc cgctgcggcg gcgcccgccg gcctgtgtcc gacgtactgc
     5341 cggccgccga gcaacccgct gatcgtgctc agcaccgtgc cgaccgattt cctcgagggc
     5401 tacgaccagg ccgacatgtg cgagcggctg ctcgagacga cccgcgacga gccgctacgg
     5461 ttcgagcgga ggatcttcga ctcggtcgat cggctcaacg attgggtcta cgacttcacg
     5521 cgcgggaagg gcgcgcaagg cgaggatctc taccgccgct gtgccggcaa gtgcagtccg
     5581 cagtacggct ggacgatctc gcagcagggc acgagcctgg ccgtcgacac cgaggtgatc
     5641 tgcggccacg cccgcgatcg ggaagtgggc cgctatacgc tgcggtccag cctgcgttgg
     5701 atctgtgacg cacggtgacc cgccgtccgg ttgacgggcc aacccgccgg aacctacgtt
     5761 tgatcggcgg agaatctcgc gaggagccgg gtgcggtggg aacagggcga gaactgagcg
     5821 ataccgatcg ccagcggctg accggcgcgc tcggggtcgt ttccgagctg atcaccggcc
     5881 ccgagctcga cgccctgcgc caggtgatcg aacagctcga gcggaagctc cgcgaggagc
     5941 tcgcggagtt gggcgggcga ctcgacggga gcctgtccga tttcaagagc gaaatcagcc
     6001 gggagaccga cagacttcag ctggcggtgg ccgcggtcga agagcggttg gccgctggta
     6061 tcgagcaggc ggatcgcgcg gtgcaaggcg cggccgaggc cgtcgagctc gagctggcct
     6121 ccgctcgtgg ggcaatcgcc gacgtcgacc gtcgctcggc cgaagatcgc cagcggctcg
     6181 accggatcgc gggtcaggtc gaccgcgcgc tggccgacgc ccccgacaag ctcgagtcga
     6241 ggttcgaggc cgtcgaggcc ggactgcgat cccacgtcga cgagcgcgtc gcccgcctcg
     6301 agcgcgcgtt tcaagcgatc gagcgcaagg tcagcgagca gacccaggcc gcgcagcgtc
     6361 tgtccgggct gctgaccaac ctcgagaccg tcttctccgc ccgacaaccg acgtcgcaga
     6421 ggagcgccgc cgaccccgac ccgacggtcg acccgaagtc ggtcgatcgc gacgcgccgc
     6481 agatgtcgag caccgagctg gacaacgcgc tgaacaacct gttccacgga tcttgactcg
     6541 ggaccgcgcc ccggttcagg cgcgatagaa gatccgcttg taccacggca cgccgcgctc
     6601 acgcgcttcg cgtcggatct cccgcgcccg ctcccgtccc ttgtcgagcg ccagcttctc
     6661 gaggtaggcc gacccgtcgg tgacgtggca gtccttgtac ttctttccgc tgccacacgg
     6721 acacgggtcg ttgcgtccca ccttgaggat gtggcgcggc gacggtgtgt tggaaaagcc
     6781 gggccagacc accttggaat gggtcctgaa tcggcggctg ctcacggcac ctccgcttcc
     6841 atcctagcgg atctcgcggc ggctccccac cctataatgg tcgcggtcgg tcgccgacgt
     6901 gtcgcaagcc gtatcgcaac ccgcccggtc cgcagcagac cggtccgtgc cggccggccg
     6961 tgtcctgttg gcgctggcgt tgatcgtcgt cgggatcggc gcggcgagat ggtggccgcg
     7021 caccgtcgac gacgcgtaca tcaccttccg ctacgccgac aacctggtgg cggggcacgg
     7081 gccggtgttc aacccgggcg agcgggtcga aggctgcagc agcccgctgt ggatgaccgg
     7141 ctcggcgctg ctgatcgcga tcgggctcga tccggtcgcg gggtcgaagt cgggcgggtt
     7201 gctggcaagc tgggtgctcg tcggcctctg ctaccgcacg gttcggcgct cgggcgtgtc
     7261 cgccgcgggc gccggcgtcg cggcgctgct gctcggatcg agtctggtgc tgcagatctg
     7321 gagcgtcgcc gggatggaaa ccaacgcgta cgcgctactc gtgttcgccg gtctggtcgc
     7381 gctgacccag ggcccgctca cccggcgggc ggcgctcgtc gcctcgctcg cgctggtcgc
     7441 ggccgcatcg acgcgtccgg agggggtcgg actgtgggtt ctcggctcgc tggttccgtg
     7501 ggtggccggc gaacgccgcc tgcgagcggg ttcggcctac ctccttcccg gcgcgatcct
     7561 cgccggttac gttctctggc gcctcggttt ctacggggac tggtggccca acacctacta
     7621 cgcgaaaacc ggcggcggcg tggcgatgtg gaagcagggc cttgacgggc tccggctgtt
     7681 cgtcacccac ccggcccacg ccccctggct cgtcgctgcc gcgatcggcg gggcgctcgc
     7741 gctgcgctcg gccggccacg tccgccaggt gctggtccac ggtgcggcgg tcggcgccca
     7801 cctgctgtgg atcgtcagcg tcggcgacga cggtttgcgc gtgcatcgat tctacgtgcc
     7861 cgtactggcc ccgctcgcga cactgagcgg gttcgtcttc cttccggcgc tcgagcggag
     7921 gttcgaccgc gcgctcgcac gatcggccat ggcgttggcc gcgatcgcga tcgtggtctc
     7981 gctgtcgacc ttccacctgc ggatggtgcc ggagatgcga cgggccctgg actaccagcg
     8041 ggggaacatc gccctcggtc gccatctcgc ggatcactac cccggcgagt cgatcgccgt
     8101 cgccgcggcg ggcgcgattc cgtactactc gggcgcgccg acgatcgaca tgtacggctt
     8161 gaacgacgcc cacatcgcac gccgaccgtt cgccgccgat cagccggggc ggatgatgaa
     8221 atgggacaac gcctacgtcc tggcgcgccg cccgcgcttc atcgtgatca accggggcta
     8281 tctcgcgccc ggcgatcggc tggcggagcg ggtcctgcgc gatccgcgac tgctcgcggt
     8341 cagcgcgctg gaccgcgacc tgttccggcg cgtcgtcagc gacggtggct acgtgccgcg
     8401 cttgctcgag ctcgacggtg gagcgggatt cgtcgtgttc gagcgtcgcc cgggtagaga
     8461 acgacgctag tccccgagcg ccacaaggtg gtctttggtg cgatagaaaa tcatcccgcg
     8521 agagatggcg ggcgtcgcca tgcagatctc gcccatttcg ttcgtcgccg acagctcgaa
     8581 cgcggcgcct gccttgacga cgtagacctc gccggtctcc gccgtggcgt agactttgcc
     8641 gtcgccggcg acgagcgacg cggtgaaacc gctgctgccc gtgccgagac gttgctggta
     8701 ctgctgctcg ccgtccagtg cgcggtagac cgtcagcacg ccgttgtcgc gcaggacgta
     8761 gagcaagccc cggtagacca acgtcgtcgg catgtaggag ccgccgccgc gccggctcca
     8821 gacgacgtga tcgttcgtct gttgaccctc gcccaacgtc acgtcgcctc tggccgtcgg
     8881 ccggatcgcg tagatcggcg acccgggacc gtgggcgctg gtgaggaaga ccagaccgtc
     8941 cgcgacgtac ggggtcggca cggggatgtc accggtgccg cgcatcgacc acaacagctc
     9001 gccgcgatcg aggtcgtagc cgccgatctg cttgaacccg ttcaccagca cctgacttcg
     9061 cgactcgccg acgtggacgg tcggcgtgct ccaggtcggg acgtcctgcc gcacggtctt
     9121 ccagatgacg tcaccggtcg cgagagcgag agcggcgaga aaggagccct gctggacgtc
     9181 ggcttggatg atgacgcgcc cgtcgtggat caccggcgag ctgccgaagc cccactgggc
     9241 ggccggcacc tggaagtagc cggagtcgag cgagccgaag tcttggttcc aaaccgactt
     9301 tccctccgtg tcgaagacgt acaacccctc cgacccgaag aaggcggcga cgtacttgcc
     9361 gtcggtcgtc ggcgacgggt tggcgtggga cgacttggtg tgccgcttga tctcgggaac
     9421 tccctggtgg accgtccgct cccagacgat cgagccggtg ttgcgatcga gacggtagag
     9481 ccggtagcgg tgctcggtgt cgtcctcgac cgcgtcgatg ttcccgtaca gcccgacctt
     9541 cagctcggga tcgggcttgc cgctgatcgc ggtcgtgacg aagatcgcgt cgccccagac
     9601 gacggggctc gagtgcccca acccggggat cgcggtcttc cacttgaccc gcgtcccgtc
     9661 ctcgacgttc caactcgccg gcagctcgtg gccgtcggcc acgccgcgcg cgccggggcc
     9721 gcgaaactgc ggccagttcc cggcgtcggt atcggcggcc ggaaccgccg cgatcgccat
     9781 tcccgtcgcg accagtgcga ggacggagct tgcgcgccgc gccatcgtcg ttcccccctg
     9841 cgtgcagacc gtctcggcac cgcgcccgct cacgcggtcc gagcggatcc cgcccttcgg
     9901 gcgtaggata gtccaagccc cggcgtcagc aactcgagct gtcgcttccc gacgccggcg
     9961 gccgcgccgc gtggaacccg ccgccaggca ggtgctagac ggtcgggcgc agccgtccga
    10021 gaaagacgtt gaccgggtgg cggcggtcga ggaacgtcaa caccggttca cgtctgacgc
    10081 cgagcttgcg caggtccgtc gcgattccct cgtgccccgg gtcgagcttc gcggccaggc
    10141 gcaacgcgcg ataggcctcg cctcgccgtc cggtcagcag agcgacccgc cccaggttcc
    10201 accagacgtc cggccggtag ccctcgagct cggcggctcg acggcagaac tggagcgcct
    10261 cgcggagctt gtgggtggac tgggccaggc acagaccgta gtacgagaga taccgcgcct
    10321 cgccctgccc cggcgacgcg ccgcgctcgg tttccagatc gatcgcggcc cgcaggtagg
    10381 ccgccgcgtc ccgcgagcgc ccgcgacgca acagctcggc accgtgctga aaggccgtct
    10441 cggcccgctg attcagcatg actcctcctc ctggtcagat gtgtcgaata tcgcgtcgcg
    10501 cgcgaggcgg gtccatagaa aatgtggtga ccgaccttac gttcccgtaa cccgcgacgg
    10561 caatcggcgt cagtcagccg gtcgagtcgt cgcgaggtcc ggttctttcc cgtcgcggag
    10621 ccgggcgccg gccgctcgca gcgacgcggc gtcacctcct ccgccgttcg acggcgctcc
    10681 gttgccgaag cgtatcgcgc gaacgagccg ggccgacagc ccggaaaagc cgcggcgagc
    10741 ggacgaagcc tcgccggttc gcacgaccgc gcgacgaaca gcggcggcgg tttccgttcg
    10801 cgcgttgctc gatgccgaca accaggcgag ggtcgcgccg agctttcgaa atcggtggtg
    10861 ggcaccgccg ttgcagattc atatcgtcat gcgaaacggc gctgcacatc gagccgaacg
    10921 agggagcgat gaagcgaatc cgacgcgatc gaccgatacg ttacctggtg gcttccgtgt
    10981 tgctcacgac gaccgcggcg gtctcgcccg tcggctcgcg cgcgtcgctc gccggtgcca
    11041 tcgacttcgc cgacctcgaa cgcgagcgcg tcgagcgggc ggcgatcctc gagctcgtcc
    11101 agcgccaccg ccccgccgac gagcgttgct ggcagcgtca cgtggcggag gtgatctaca
    11161 gcgaatccaa ggccgtcggc atcgatccgt tgctcaccgc cgcgatcgtc gcccgcgaga
    11221 gttcgttccg cagccgcgcc gagagcgagg ccggcgcgtt gggcctgatg caggtccgac
    11281 cgttcgtcgc gcaggacgtg gcgctgcggc gcggcgtcgc ctggggcggc agcgaaacgc
    11341 tgcacgaccc gcgcctcaac gtgcgactgg gcacgtcgta ctacggggaa ctgctgtcac
    11401 gattcgacgg cgacgtgacg ctggcgctcg cggcctacca ccgcggcccg acccgtctca
    11461 gccggcagat tcgcgagggg agcttctccg gcgcgccgta tgccggccgg gtgctgcact
    11521 tgtacgcccg cctcgacgcc gagcggcagg aacgactcgg tcaggccggg ctgtagtccg
    11581 ttcaccgggc tcaggcgagg ccgcgcgcgc cgatgaccgc cgggatcgcc gactggcagt
    11641 gcgggcagac caccaggtag ccccgtcgag gaagcgacga ggtgaagttc accttcttgc
    11701 ggaggaggcg gatgacctgg actgtctcga actgttgccc gcaatgcgcg cagcggaccg
    11761 acgattccag ctgatactcg gcttcgcact cgatctcgtg acccagcgtt tccatcggca
    11821 ccctccgcgc ctgccctcgc agctcgtaca gggaatggtt aggttccgga aagaccgcgg
    11881 tcaagccgga gcgtgcggct cacccgccga acggccgcga gcacggcgtc gaccgacagc
    11941 tcccgcaggc agcggtgatg accgagcggg caggcccgct cgaaacaggg gctgcagtcg
    12001 agaccgaggt acagcacctc ggcgcggtcg gtgagtggtg gcgtgtaaac aggcgacgac
    12061 gaaccgtaaa gggccacgac gtggctgccg accgctgccg cgacgtgcat cagtccggtg
    12121 tcgttactga ccacggtcga ggtgagcgac agcaagtcga cggcgtcctc gagccggagc
    12181 tcgccgcaca ggtttcgcgc gcggggcccg gcgagggcgc ggacgcgctc gcccgtttct
    12241 cgctccgcgg cgccgccgag gatccagacc gcgacgccgg acgcggtcag ccggcgcccg
    12301 agttcgccga acgactcggc cggccaggtc ttggccgggc cgtactccgc gcccggcacg
    12361 caggcgacga cgggtcgctc ggaggcgagc ccgagccgtg cggcgacccg gccgcgattg
    12421 tcgcggtcga cccgcagcgc gggacggggg atctcctcgg cgaccgtgcc gcccgcccgc
    12481 ggggccagtg ccagatagcg ccgcaccatc ggtcgatcga agctgacatc gagcggccgc
    12541 gcgtcgttga tcaagccgaa gcgttgctcg ccgcggtagc cggtgcggac cggcacgcgc
    12601 gcgaagaacg gaacgagcga ggacttccac gacttgggca tcacgatcgc gcggtcgtag
    12661 cgctcgccct gcagcgagcg accgatgcgc cagcgcgcgg cgaaccccaa tcggccgtgc
    12721 gcaacgggga gaccgatcgc gcgccggatc tcgggcatgc gaccgagtat cggtcgggac
    12781 cagtccggag cgacgacgtc gatcgtcacg ctcgggtcgt cccgtcgcag ttggaggaac
    12841 aggctctgcg ccatcaccat gtcgccgatc cacgacggtc cgaccacgag caaacgcgcg
    12901 ccgccggccg ggccggaccg ggcgtcgacc ggcctgcctg tcctctcgtc gttgaccacc
    12961 gttcggattc tagcctgcac gagggccagc gggttcatcg cgacaacggc ccgggagcga
    13021 gcacgggacc gtcgtccggc cacggcctgc ggcaggcggt tcgcgaacga tagccgcgca
    13081 tgttcccccg gagctcctcg atcaacgccg cgggcgctcc gtcgccgatc gcggcgagtg
    13141 cccggctctg ccaatcgatg gcggcgtcga aatccccgtt ctcggccgcc gccatcgcca
    13201 ccgcctccag cacgtcgaac gaagcgtccg gcgcgacaag cgtacgcgcc aggcgctcgg
    13261 cccgccggcc gtcgcggacg gcgtcgtccg gtgcggccgc cagcaggcgg acgacggcct
    13321 gtaggatgtt gcgatcgccg ggaatcgcct cgcgcgccgc atcgaggcgg tgcagtgcct
    13381 cggcataccg ctgcaggcgg atcaacacca gcccctcgtt caatcgtgcc gcggcatgcc
    13441 ccggctgccg atcgatgacc cagtggtact cggccagcgc ttgctcgaaa cggccgaggc
    13501 gtcgcagcgc attgcccagg ttgacgtgcg ccggtaccag gccggggtcg atgtcgagcg
    13561 cggcgcgcag gtgcgccacc gctcgctcgt catctcccgt ttgagcgtag accgtgccga
    13621 gattgaactg ggccgtcgcg gatccggggt cgaggctcag cgcgcgttcg agctcggcgc
    13681 gcgcgccgtc gaggtcgccg gcctgcgcca ggccgtgtcc cagcgtggtg cgggccgtca
    13741 cgtcctcggg cgcggcttcg accgcgcggc gcagctcggc cagtgcctgc gcgtgacgcc
    13801 cctcggccga cagccgtaac ccgcggtcga ggtgagagag catgtcccgg cggtaccgtt
    13861 gcacccggtc gagccacgga tcgtcgacgg atggcgggac cccgccctgc ccgtcgagca
    13921 atggcaacgc gcgatcgatc cgccccagac cccggtaggc cagaccgagc gggtaacgga
    13981 tggcggtcgc cgacggcgcg agcgcgacgg cggcttccag tagctcgacc gcgtggtcgt
    14041 agtcgccttc ggcgagcgcg atcttgccca gcccgaccca cgcggccgcg ctgtcgggat
    14101 cggcgtcgag cgccgcggcg aaccaggccg caccgtcccg gtaacggccg cgctcgctct
    14161 cggcgcggcc gagctcgatc agcgtcggca cgtagctcgg gtcgtcggca acgctccgct
    14221 cgaaggccgc gatcgcgtcg tcctgggccc ccgcgtcgcg gtacagccgt ccgaggtagt
    14281 acggccagcg cggttcgctc ggatcgagac gctcggcgtt gcgatagcac gggatcgccg
    14341 cggcagcgag gcgataggcg tggtaggtca tgccgagctc gccgaacgcc ctgccgaggt
    14401 cggcagcgct cgaccgcggg tcggcggccg tcgtctcgac cgcgcctctc gtctgttcga
    14461 tctgctcgag cacgaccggg tccgagccgg ccagcgccgg atgcggcagc ggctcgagcg
    14521 aacggtcggt ctcgcctcgc ggggagcggc aggcggcgca gatcgatagc gccgtcagcg
    14581 acaggacgag cagctgtcgt cggcgcgggc tcacctggcg gcgacccgcc cggaaccgcg
    14641 ctcgatcccc gtgtagcgac cgatcgcggg ggcttcccag tcctcgatcg tgccgtcggg
    14701 ccacaccgcg cgcagggaga cgacgcccga tgcgccgccc aacccgaaca gcacgcgcgg
    14761 atcgttcgac gaaaggtagc tcgccgcggt tcgcacacga cggacgagcg accgcccgtc
    14821 ctccaggcgc aagctgacct cggttccgat cacgtcgtac ctgccctcgg ggtcggtcat
    14881 ccgcacgccg acccagcgat tgcgctggcc caccaggttg agcaacagac ggagcgggcc
    14941 gctgttgttg gcgacgacga cgtcggtgtc gccgtcgttg tccaggtcgc cgaaggccgc
    15001 cccgcgtccg acctcggaca gctcgaacgc cggtccggcc acccggctgc gctcggcgaa
    15061 gcttccgtca ccgagattga ggaagacctg gtcccgttcg tccagcggga acgggtcgcc
    15121 cgcgcgggcg agttcctcga tgtgcttgac cgcaccgttg accagcagca ggtcgagcca
    15181 gccgtcgttg tcgatgtcga cccaggccgt gccgaacccg gtgaaaccac ggctggcggt
    15241 gcccagccc
//
